I/O Summit Europe 2024
Hilton London Canary Wharf S Quay Square, Marsh Wall, London, United KingdomJoin Discovery Life Sciences at I/O Summit Europe 2024 April 23-25, 2024 | Hilton London Canary Wharf, London, England
Discovery has worked with hundreds of researchers on thousands of projects to help uncover valuable insights that accelerate research and development programs across several disease indications, including cancer, infectious disease, autoimmune disease, women’s health, cardiovascular disease, inflammatory diseases and many others.
Year | Authors | Title | Journal | PubMed ID | Snippet | Link |
---|---|---|---|---|---|---|
2023 | Bellet-Ezquerra, M;Pardo, P;Fasani, R;Villacampa, G;Portu, M;Serna, G;Joval, L;Petit, A;Ortega, A;Jimenez, J;Zamora, E;Gil, M;Gomez, L;Viaplana, C;Gallego, F;Cámara, V;Manich, C;Simon, S;Nuciforo, P; | 62P KI67 in ER+HER2-negative pure invasive lobular breast carcinoma (ILC). What’s the best Ki67 threshold to differentiate prognosis? | ESMO Open | a, Novartis, Daiichi Sankyo; Non-Financial Interests, Invited Speaker: SOLTI. P.G. Nuciforo: Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, | Full Paper | |
2023 | Cieniewicz, B;Bhatta, A;Torabi, D;Baichoo, P;Saxton, M;Arballo, A;Nguyen, L;Thomas, S;Kethar, H;Kukutla, P;Shoaga, O;Yu, B;Yang, Z;Fate, M;Oliveira, E;Ning, H;Corey, L;Corey, D; | Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti-tumor responses and phagocytic uptake of tumor-associated antigen for T cell cross-presentation | Molecular therapy : the journal of the American Society of Gene Therapy | 37194236 | Healthy donor T cells were positively selected from fresh leukopak provided by AllCells (Alameda, CA) by using anti-CD4 and CD8 antibodies through the Prodigy TCT program. All related reagents and instruments were purchased from Miltenyi. After selec | Full Paper |
2023 | Overbeck, TR;Reiffert, A;Schmitz, K;Rittmeyer, A;Körber, W;Hugo, S;Schnalke, J;Lukat, L;Hugo, T;Hinterthaner, M;Reuter-Jessen, K;Schildhaus, HU; | NTRK Gene Fusions in Non-Small-Cell Lung Cancer: Real-World Screening Data of 1068 Unselected Patients | Cancers | 37296928 | r or speaker from: Amgen, AstraZeneca, Bayer, Daiichi-Sankyo, Diaceutics, Incyte, Janssen-Cilag, Eli Lilly, Merck Sharp and Dohme, Novartis Pharma, Pfizer, PharmaMar, Roche Pharma, Servier, Stemline, Targos GmbH, and ZytoVision. A.R. received grants | Full Paper |
2023 | Wagner, R;McKindles, K;Bullerjahn, G; | Effects of water movement and temperature on Rhizophydium infection of Planktothrix in a shallow hypereutrophic lake | Frontiers in Microbiology | sent to HudsonAlpha Discovery Life Sciences (Hudson, AL, United States) for sequencing. Amplicon sequencing and QC was performed by HudsonAlpha Discovery Life Save;- Discovery Life Sciences (Hudson, AL, United States) for sequencing. Amplicon se | Full Paper | |
2023 | de Vries, EGE;Rüschoff, J;Lolkema, M;Tabernero, J;Gianni, L;Voest, E;de Groot, DJA;Castellano, D;Erb, G;Naab, J;Donica, M;Deurloo, R;van der Heijden, MS;Viale, G; | Phase II study (KAMELEON) of single-agent T-DM1 in patients with HER2-positive advanced urothelial bladder cancer or pancreatic cancer/cholangiocarcinoma | Cancer medicine | 37119523 | Department of Medical Oncology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.++Targos Molecular Pathology GmbH, Kassel, Germany.++Department of Medical Oncology, Erasmus MC Cancer Institute, Rotterdam, The | Full Paper |
2023 | Wang, S;Huang, Z;Liu, Y;Sun, H;Zhou, Y;Shi, J; | Sustainably released nanoparticle-based rhynchophylline limits pulmonary fibrosis by inhibiting the TEK-PI3K/AKT signaling pathway | Translational lung cancer research | 37057119 | /tlcr-22-675/rc). Methods Solution groups For better apply to the clinic, 0.9% saline solution was used as the solvent. Because of the extremely low concentration for cell, 1 mg of Rhy (CAS 76-66-4; Allcells, Shanghai, China) was directly dissolved | Full Paper |
2023 | Naik, NN;Vadloori, B;Poosala, S;Srivastava, P;Coecke, S;Smith, A;Akhtar, A;Roper, C;Radhakrishnan, S;Bhyravbhatla, B;Damle, M;Pulla, VK;Hackethal, J;Horland, R;Li, AP;Pati, F;Singh, MS;Occhetta, P;Bisht, R;Dandekar, P;Bhagavatula, K;Pajkrt, D;Johnson, M;Weber, T;Huang, J;Hysenaj, L;Mallar, B;Ramray, B;Dixit, S;Joshi, S;Kulkarni, M; | Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Third International Conference on 3Rs Research and Progress, Vishakhapatnam, 2022 | Alternatives to laboratory animals : ATLA | 37282515 | Dr Li highlighted the discovery focus of Discovery Life Sciences LLC, namely the liver and intestine. He further described the properties of their two major novel human in Save | Full Paper |
2023 | Guler, S;DiPoto, MC;Crespo, A;Caldwell, R;Doerfel, B;Grossmann, N;Ho, K;Huck, B;Jones, CC;Lan, R;Musil, D;Potnick, J;Schilke, H;Sherer, B;Simon, S;Sirrenberg, C;Zhang, Z;Liu-Bujalski, L; | Selective Wee1 Inhibitors Led to Antitumor Activity In Vitro and Correlated with Myelosuppression | ACS medicinal chemistry letters | 37197456 | We acknowledge Bettina Hoelke, Brian Dill, Ioannis Gounaris, and Emer Clarke (ReachBio) for their technical support and discussions | Full Paper |
2023 | Tang, PZ;Ding, B;Reyes, C;Papp, D;Potter, J; | Target-seq: single workflow for detection of genome integration site, DNA translocation and off-target events | BioTechniques | 37161298 | Peripheral blood mononuclear cells (PBMCs) were purified from leukopak (ALLCELLS, CA, USA) using CTS™ Rotea™ Counterflow Centrifugation System (Thermo Fisher Scientific, MA, USA) and were then activated for 3 days to enrich the T cells (at 1 × 106 ce | Full Paper |
2023 | Singh, P; | MSC and HSPC Coculture: Mimicking Ex Vivo Bone Marrow Niche | Methods in molecular biology (Clifton, N.J.) | 36255702 | CD34+ cells with micromagnetic beads-conjugated CD34 antibody and placing with the labeled cells into a magnetic field according to the specific manual instruction. Always use fresh BM samples (within 30 h of withdrawal) for CD34+ cell isolation. Hum | Full Paper |
2023 | Simmons, KT;Chan, J;Hussain, S;Rose, EL;Markham, K;Byun, TS;Panicker, S;Parry, GC;Storek, M; | Anti-C1s humanized monoclonal antibody SAR445088: A classical pathway complement inhibitor specific for the active form of C1s | Clinical immunology (Orlando, Fla.) | 37149117 | First, 1 mL of type O- hRBCs (Allcells, Alameda, CA) was washed in 4.5 mL GVB++ by centrifuging at 600 g for 5 min. The wash step was repeated three times and the cells were resuspended to a final volume of 5 mL in GVB++ after the third wash | Full Paper |
2023 | Sikkema, L;Ramírez-Suástegui, C;Strobl, DC;Gillett, TE;Zappia, L;Madissoon, E;Markov, NS;Zaragosi, LE;Ji, Y;Ansari, M;Arguel, MJ;Apperloo, L;Banchero, M;Bécavin, C;Berg, M;Chichelnitskiy, E;Chung, MI;Collin, A;Gay, ACA;Gote-Schniering, J;Hooshiar Kashani, B;Inecik, K;Jain, M;Kapellos, TS;Kole, TM;Leroy, S;Mayr, CH;Oliver, AJ;von Papen, M;Peter, L;Taylor, CJ;Walzthoeni, T;Xu, C;Bui, LT;De Donno, C;Dony, L;Faiz, A;Guo, M;Gutierrez, AJ;Heumos, L;Huang, N;Ibarra, IL;Jackson, ND;Kadur Lakshminarasimha Murthy, P;Lotfollahi, M;Tabib, T;Talavera-López, C;Travaglini, KJ;Wilbrey-Clark, A;Worlock, KB;Yoshida, M;Lung Biological Network Consortium, ;van den Berge, M;Bossé, Y;Desai, TJ;Eickelberg, O;Kaminski, N;Krasnow, MA;Lafyatis, R;Nikolic, MZ;Powell, JE;Rajagopal, J;Rojas, M;Rozenblatt-Rosen, O;Seibold, MA;Sheppard, D;Shepherd, DP;Sin, DD;Timens, W;Tsankov, AM;Whitsett, J;Xu, Y;Banovich, NE;Barbry, P;Duong, TE;Falk, CS;Meyer, KB;Kropski, JA;Pe'er, D;Schiller, HB;Tata, PR;Schultze, JL;Teichmann, SA;Misharin, AV;Nawijn, MC;Luecken, MD;Theis, FJ; | An integrated cell atlas of the lung in health and disease | Nature medicine | 37291214 | 19-ms2/?ds=full&meta=SampleName, https://figshare.com/articles/dataset/Single-cell_RNA-Seq_of_human_primary_lung_and_bronchial_epithelium_cells/11981034/1, https://covid19.lambrechtslab.org/downloads/Allcells.counts.rds, https://s3.amazonaws.com/dp-l | Full Paper |
2023 | Sternberg, CN;Petrylak, DP;Bellmunt, J;Nishiyama, H;Necchi, A;Gurney, H;Lee, JL;van der Heijden, MS;Rosenbaum, E;Penel, N;Pang, ST;Li, JR;García Del Muro, X;Joly, F;Pápai, Z;Bao, W;Ellinghaus, P;Lu, C;Sierecki, M;Coppieters, S;Nakajima, K;Ishida, TC;Quinn, DI; | FORT-1: Phase II/III Study of Rogaratinib Versus Chemotherapy in Patients With Locally Advanced or Metastatic Urothelial Carcinoma Selected Based on FGFR1/3 mRNA Expression | Journal of clinical oncology : official journal of the American Society of Clinical Oncology | 36240478 | ted prevalence of PIK3CA and/or RAS resistance mutations, we reconfirmed absence or presence in all enrolled patients using a targeted Illumina MiSeq panel (Illumina, Inc, San Diego, CA) performed by TARGOS Molecular Pathology GmbH (Kassel, Germany). | Full Paper |
2023 | Ho, C;Fromm, J;Fang, M;Till, B;Shadman, M;Cowan, A;Lynch, R;Wu, V;Voutsinas, J;Rasmussen, H;Blue, K;Ujjani, C;Shustov, A;Cassaday, R;Gopal, A;Smith, S; | Long-term efficacy and safety of pembrolizumab with R-CHOP as first-line therapy for DLBCL. | Journal of Clinical Oncology | Additionally, prognostic factors including tumor PD-L1 expression (Qualtek/Merck) were re-analyzed. Results: 30 patients were treated (13/30 with IPI 3-5). Median follow Save | Full Paper | |
2023 | Zhang, Y;Zhao, Z;Huang, LA;Liu, Y;Yao, J;Sun, C;Li, Y;Zhang, Z;Ye, Y;Yuan, F;Nguyen, TK;Garlapati, NR;Wu, A;Egranov, SD;Caudle, AS;Sahin, AA;Lim, B;Beretta, L;Calin, GA;Yu, D;Hung, MC;Curran, MA;Rezvani, K;Gan, B;Tan, Z;Han, L;Lin, C;Yang, L; | Molecular mechanisms of snoRNA-IL-15 crosstalk in adipocyte lipolysis and NK cell rejuvenation | Cell metabolism | 37329887 | Healthy and obese human serum Coriell Biorepository See Table S1 Human breast cancer tumor tissues and paired plasma samples Discovery Life Sciences See Table S2 Primary human pre-adipocytes Zen-Bio See Table S3 | Full Paper |
2023 | Libreros, S;Nshimiyimana, R;Lee, B;Serhan, CN; | Infectious Neutrophil deployment is regulated by Resolvin D4 | Blood | 37295018 | Fresh BM aspirates from deidentified individuals were purchased from AllCells, Inc (Alameda, CA) with Mass General Brigham investigational review board protocol no. 1999P0001279. For information on donor demographics, see Supplemental Table-1. Human | Full Paper |
2023 | Dunbar, AJ;Kim, D;Lu, M;Farina, M;Bowman, RL;Yang, JL;Park, Y;Karzai, A;Xiao, W;Zaroogian, Z;O'Connor, K;Mowla, S;Gobbo, F;Verachi, P;Martelli, F;Sarli, G;Xia, L;Elmansy, N;Kleppe, M;Chen, Z;Xiao, Y;McGovern, E;Snyder, J;Krishnan, A;Hill, C;Cordner, K;Zouak, A;Salama, ME;Yohai, J;Tucker, E;Chen, J;Zhou, J;McConnell, T;Migliaccio, AR;Koche, R;Rampal, R;Fan, R;Levine, RL;Hoffman, R; | CXCL8/CXCR2 signaling mediates bone marrow fibrosis and is a therapeutic target in myelofibrosis | Blood | 36800567 | ple was independently verified by a hematopathologist (W.X.) or pulled directly from clinical pathology reports at the time of sample collection. Deidentified, healthy CD34+ cells were purchased from AllCells. CD34+ selection was carried out using Fi | Full Paper |
2023 | Kiridena, S;Wijayaratna, U;Levon, E;Moschella, P;Pirrallo, R;Tzeng, T;Anker, J; | X?Ray Visualized Sensors for Peritoneal Dialysis Catheter Infection | Advanced Functional Materials | Deidentified human peritoneal fluid was obtained from Discovery Life Sciences, Huntsville, AL, a commercial biospecimen source. Tantalum beads (0.394 mm diameter) Save | Full Paper | |
2023 | Tarafder, S;Ghataure, J;Langford, D;Brooke, R;Kim, R;Eyen, SL;Bensadoun, J;Felix, JT;Cook, JL;Lee, CH; | Advanced bioactive glue tethering Lubricin/PRG4 to promote integrated healing of avascular meniscus tears | Bioactive materials | 37214259 | ?S-encapsulating TGF?3 (R&D systems) (2.5 ?g per 500 mg PLGA) was applied to the incised tissues. Then the meniscus explants were placed on top of monolayer-cultured P2 - P3 syMSCs or hBMSCs (AllCells, Alameda, CA) at 80-90% confluence per our previo | Full Paper |
2023 | Sarno, J;Domizi, P;Liu, Y;Merchant, M;Pedersen, CB;Jedoui, D;Jager, A;Nolan, GP;Gaipa, G;Bendall, SC;Bava, FA;Davis, KL; | Dasatinib overcomes glucocorticoid resistance in B-cell acute lymphoblastic leukemia | Nature communications | 37217509 | Healthy human bone marrow samples (n = 3 for RNA-Seq, n = 4 for CyTOF) were purchased through AllCells. De-identified bone marrow samples from pediatric patients Save | Full Paper |
2023 | Yang, Y;Yang, H;Alcaina, Y;Puc, J;Birt, A;Vedvyas, Y;Gallagher, M;Alla, S;Riascos, MC;McCloskey, JE;Du, K;Gonzalez-Valdivieso, J;Min, IM;de Stanchina, E;Britz, M;von Hofe, E;Jin, MM; | Inducible expression of interleukin-12 augments the efficacy of affinity-tuned chimeric antigen receptors in murine solid tumor models | Nature communications | 37045815 | quoted and stored at ?80?°C. CAR-T cells were generated by transducing activated T cells with lentivirus. Primary T cells were enriched from commercially obtained leukopaks from healthy donors (AllCells) via MACS separation using CD4 (Miltenyi Biote | Full Paper |
2023 | Lee, JY;Jonus, HC;Sadanand, A;Branella, GM;Maximov, V;Suttapitugsakul, S;Schniederjan, MJ;Shim, J;Ho, A;Parwani, KK;Fedanov, A;Pilgrim, AA;Silva, JA;Schnepp, RW;Doering, CB;Wu, R;Spencer, HT;Goldsmith, KC; | Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastoma | Cell reports. Medicine | 37343516 | Bacterial and virus strains ------------------------- Stbl3 Competent Cells Spencer Lab N/A ------------------------- Biological samples ------------------------- Healthy PBMC AllCells LP,CR,MNC, 10M COG-424s (Neuroblastoma PDX) Chi | Full Paper |
2023 | Chen, SS;Barrientos, JC;Ferrer, G;King-Richards, M;Chen, YJ;Ravichandran, P;Ibrahim, M;Kieso, Y;Waters, S;Kutok, JL;Peluso, M;Sharma, S;Weaver, DT;Pachter, JA;Rai, KR;Chiorazzi, N; | Duvelisib Eliminates CLL B Cells, Impairs CLL-Supporting Cells, and Overcomes Ibrutinib Resistance in a Xenograft Model | Clinical cancer research : an official journal of the American Association for Cancer Research | 37071496 | Human PBMCs obtained from whole blood of CLL donors were purchased from a commercial source (Bioreclamation IVT or ALLCELLS) were resuspended (1 × 10 6 cells/ Save | Full Paper |
2023 | Xiang, J;Liu, Q;Shaknovich, R;Venn, O; | Abstract 779: Clonal B-cell expansion and the potential challenges to blood-based early cancer detection | Cancer Research | ht tubes of frozen buffy coat for a young healthy donor (18-35; no pre-existing health conditions; nonsmoker; viral negative) was sourced commercially (Discovery Life Sciences, Huntsville, AL, USA) and used as the backgrou | Full Paper | |
2023 | Fahl, S;Colwell, K;Smith, C;Ott, C;Moore, A;Williams, K; | Abstract 602: Single cell evaluation of intratumoral B cells across solid tumor indications | Cancer Research | 1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsvill | Full Paper | |
2023 | Li, A;Wei, H; | Abstract 5338: A novel in vitro assay to evaluate the roles of hepatic metabolism on anticancer drug safety and efficacy | Cancer Research | 1Discovery Life Sciences LLC, Columbia, MD.++1Discovery Life Sciences LLC, Columbia, MD | Full Paper | |
2023 | Roy, G;Kadekar, P;Douglas, L;Frappier, M;Currie, J;Dhillon, J;Cesarone, G;Siderits, R;Kirchner, K;Demeule, M;Marsolais, C; | Abstract 3942: Differential expression of a novel transport receptor, SORT1 (sortilin), in cancer versus healthy tissues that can be utilized for targeted delivery of anti-cancer drugs | Cancer Research | 1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, | Full Paper | |
2023 | Cheng, C;Chen, H;Kirchner, K;Cesarone, G; | Abstract 2763: Characterization of a novel immunohistochemistry (IHC) assay for CEACAM5 using a commercial antibody | Cancer Research | [Abcam], and 327 [Sino Biological]) to evaluate CEACAM5 detection in formalin-fixed, paraffin-embedded (FFPE) human cancer tissues from Discovery Life Sciences Save | Full Paper | |
2023 | Zielinski, D;Vogtmann, R;Siemon, A;Koppel, C;Schipper, C;Tereshchenko, A;Platero, S;Schildhaus, H; | Abstract 1018: Multiplex immunofluorescence and image analysis to investigate the role of the immune contexture and fibroblast activation for tumor cell budding in colorectal cancer | Cancer Research | 1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsvill | Full Paper | |
2023 | Kumar, A;Taghi Khani, A;Duault, C;Aramburo, S;Sanchez Ortiz, A;Lee, SJ;Chan, A;McDonald, T;Huang, M;Lacayo, NJ;Sakamoto, KM;Yu, J;Hurtz, C;Carroll, M;Tasian, SK;Ghoda, L;Marcucci, G;Gu, Z;Rosen, ST;Armenian, S;Izraeli, S;Chen, CW;Caligiuri, MA;Forman, SJ;Maecker, HT;Swaminathan, S; | Intrinsic suppression of type I interferon production underlies the therapeutic efficacy of IL-15-producing natural killer cells in B-cell acute lymphoblastic leukemia | Journal for immunotherapy of cancer | 37217248 | opoietic Tissue Biorepository and University of Pennsylvania Stem Cell and Xenograft Core after informed consent per Institutional Review Board policies. Age-matched healthy BMMCs were purchased from AllCells (Alameda, California, USA) and Stem Cell | Full Paper |
2023 | Nirschl, CJ;Brodkin, HR;Domonkos, C;Dwyer, CJ;Hicklin, DJ;Ismail, N;Seidel-Dugan, C;Steiner, P;Steuert, Z;Sullivan, JM;Winston, WM;Salmeron, A; | mWTX-330, an IL 12 INDUKINE Molecule, Activates and Reshapes Tumor-infiltrating CD8+ T and NK Cells to Generate Antitumor Immunity | Cancer immunology research | 37074216 | Dissociated human tumor samples were purchased from Discovery Life Sciences and included the following indications: bladder cancer, glioblastoma multiforme, gastric Save | Full Paper |
2023 | Diwanji, R;O'Brien, NA;Choi, JE;Nguyen, B;Laszewski, T;Grauel, AL;Yan, Z;Xu, X;Wu, J;Ruddy, DA;Piquet, M;Pelletier, MR;Savchenko, A;Charette, L;Rodrik-Outmezguine, V;Baum, J;Millholland, JM;Wong, CC;Martin, AM;Dranoff, G;Pruteanu-Malinici, I;Cremasco, V;Sabatos-Peyton, C;Jayaraman, P; | Targeting the IL1? Pathway for Cancer Immunotherapy Remodels the Tumor Microenvironment and Enhances Antitumor Immune Responses | Cancer immunology research | 37040466 | Dissociated human microsatellite-stable colorectal carcinoma (MSS-CRC) samples were obtained from Discovery Life Sciences from five donors. Between 25,000 and Save | Full Paper |
2023 | Huang, W;Lin, W;Chen, B;Zhang, J;Gao, P;Fan, Y;Lin, Y;Wei, P; | NFAT and NF-?B dynamically co-regulate TCR and CAR signaling responses in human T cells | Cell reports | 37347664 | K562 Laboratory of Wendell Lim N/A Jurkat Laboratory of Arthur Weiss N/A Human PBMC ALLCELLS PB004F-C | Full Paper |
2023 | Raj, N;Chan, JA;Wang, SJ;Aggarwal, RR;Calabrese, S;DeMore, A;Fong, L;Grabowsky, J;Hope, TA;Kolli, KP;Mulvey, CK;Munster, PN;Perez, K;Punn, S;Reidy-Lagunes, D;Von Fedak, S;Zhang, L;Bergsland, EK; | Pembrolizumab alone and pembrolizumab plus chemotherapy in previously treated, extrapulmonary poorly differentiated neuroendocrine carcinomas | British journal of cancer | 37208512 | Background To date, single-agent immune checkpoint inhibitor (CPI) therapy has proven to be ineffective against biomarker-unselected extrapulmonary poorly Save | Full Paper |
2023 | Reissig, TM;Tzianopoulos, I;Liffers, ST;Rosery, VK;Guyot, M;Ting, S;Wiesweg, M;Kasper, S;Meister, P;Herold, T;Schmidt, HH;Schumacher, B;Albers, D;Markus, P;Treckmann, J;Schuler, M;Schildhaus, HU;Siveke, JT; | Smaller panel, similar results: genomic profiling and molecularly informed therapy in pancreatic cancer | ESMO open | 37148593 | stock ownership: none; honoraries for CME presentations: Amgen, Boehringer Ingelheim, Bristol-Myers Squibb, Janssen, Novartis; research funding to institution: AstraZeneca, Bristol Myers-Squibb. HUS: Targos Molecular Pathology, Inc. (employment); Roc | Full Paper |
2023 | Han, R;Guo, H;Shi, J;Wang, H;Zhao, S;Jia, Y;Liu, X;Li, J;Cheng, L;Zhao, C;Li, X;Zhou, C; | Tumour microenvironment changes after osimertinib treatment resistance in non-small cell lung cancer | European journal of cancer (Oxford, England : 1990) | 37320935 | Fresh PBMCs of three healthy donors were purchased from Allcells (China). A total of 2 × 10 6 PBMC cells/well were seeded into 24-well plated and were treated with: Save | Full Paper |
2023 | Geoerger, B;Marshall, LV;Nysom, K;Makin, G;Bouffet, E;Defachelles, AS;Amoroso, L;Aerts, I;Leblond, P;Barahona, P;Van-Vlerken, K;Fu, E;Solca, F;Lorence, RM;Ziegler, DS; | Afatinib in paediatric patients with recurrent/refractory ErbB-dysregulated tumours: Results of a phase I/expansion trial | European journal of cancer (Oxford, England : 1990) | 37178647 | Biomarker assays were performed centrally by TARGOS Molecular Pathology GmbH using tissue samples from archived tumour blocks, or tissue sections [20]. Save | Full Paper |
2023 | Robert, ME;Rüschoff, J;Jasani, B;Graham, RP;Badve, SS;Rodriguez-Justo, M;Kodach, LL;Srivastava, A;Wang, HL;Tang, LH;Troncone, G;Rojo, F;Van Treeck, BJ;Pratt, J;Shnitsar, I;Kumar, G;Karasarides, M;Anders, RA; | Erratum to High Interobserver Variability Among Pathologists Using Combined Positive Score to Evaluate PD-L1 Expression in Gastric, Gastroesophageal Junction, and Esophageal Adenocarcinoma [Modern Pathology 36(5) (2023) 100154] | Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc | 37327723 | Yale University School of Medicine, New Haven, Connecticut. Electronic address: marie.robert@yale.edu.++Discovery Life Sciences, Hesse, Germany.++Discovery Life Sciences, Hesse, Germany.++Mayo Clinic, Rochester, Minnesota.++Emory University School of | Full Paper |
2023 | Dong, Q;Tong, M;Yu, X;Wang, L;Ao, J;Guan, D;Tang, Y;Liu, J;Long, L;Tong, Y;Fang, S;Zhou, H;Huang, Y;Gong, L;Lou, L;Huang, W; | Carbohydrate Strengthens the Immunotherapeutic Effect of Small-Molecule PD-L1 Inhibitors | Journal of medicinal chemistry | 37226718 | Stimulator cells from donor A and responder cells from donor B constitute the mixed lymphocyte reaction (MLR). Pheral Blood Mononuclear Cells (PBMCs) were purchased from Allcells (Shanghai) and used following the instruction of the manufacturer. DC c | Full Paper |
2023 | Papakonstantinou, A;Napoli, S;Joval, L;Butjosa-Espin, M;Malavila, A;Puy, M;Pimentel, I;Zamora, E;Torres, P;Rodas, N;Manich, C;Seoane, J;Nonell, L;Nuciforo, P;Oliveira, M; | 19P Tumor-associated microbiome composition and response to neoadjuvant chemotherapy (NACT) in early triple-negative breast cancer (TNBC) | ESMO Open | ncer Research (AACR). P.G. Nuciforo: Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. M. O | Full Paper | |
2023 | Akiyama, H;Carter, BZ;Andreeff, M;Ishizawa, J; | Molecular Mechanisms of Ferroptosis and Updates of Ferroptosis Studies in Cancers and Leukemia | Cells | 37190037 | Another study demonstrates that progestin and adipoQ receptor 3 (PAQR3) enhances erastin- as well as RSL3-induced ferroptosis in ALLcells through the degradation of Save | Full Paper |
2023 | Chaand, M;Fiore, C;Johnston, B;D'Ippolito, A;Moon, DH;Carulli, JP;Shearstone, JR; | Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressor | Communications biology | 37316562 | Fetal CB-derived CD34+ cells (AllCells, LLC) were transduced with concentrated lentivirus at an MOI = 1. Transduced cells were selected in the presence of 2 µg/mL Save | Full Paper |
2023 | Lin, W;Singh, V;Springer, R;Choonoo, G;Gupta, N;Patel, A;Frleta, D;Zhong, J;Owczarek, T;Decker, C;Macdonald, L;Murphy, A;Thurston, G;Mohrs, M;Ioffe, E;Lu, YF; | Human CD4 cytotoxic T lymphocytes mediate potent tumor control in humanized immune system mice | Communications biology | 37185301 | from fetal liver or cord blood, respectively. Fetal liver CD34+ HSPC were obtained from Advanced Biosciences Resources (Alameda, CA) with proper consent66 and cord blood CD34+ HSPC were obtained from AllCells, HemaCare, or STEMCELL Technologies. Huma | Full Paper |
2023 | Maden, M;Polvadore, T;Polanco, A;Barbazuk, WB;Stanley, E; | Osteoderms in a mammal the spiny mouse Acomys and the independent evolution of dermal armor | iScience | 37378333 | na NovaSeq6000 platform to yield nearly 898 million paired-end, 150 bp reads (mean of 74.8 M read pairs (s = 9.1 M read pairs) per library). Library construction and sequencing were performed by HudsonAlpha Discovery in Huntsville, Alabama, USA. Qua | Full Paper |
2023 | Egelston, CA;Guo, W;Yost, SE;Ge, X;Lee, JS;Frankel, PH;Cui, Y;Ruel, C;Schmolze, D;Murga, M;Tang, A;Martinez, N;Karimi, M;Somlo, G;Lee, PP;Waisman, JR;Yuan, Y; | Immunogenicity and efficacy of pembrolizumab and doxorubicin in a phase I trial for patients with metastatic triple-negative breast cancer | Cancer immunology, immunotherapy : CII | 37294342 | Biomarker Working Group on Breast Cancer Guidelines [18]. PD-L1 was determined by QualTek Molecular Laboratory (Goleta, CA) using immunohistochemistry (IHC) with 22C3 antibody (Merck & Co, Kenilworth, NJ). PD-L1 was positive if membrane staining was | Full Paper |
2023 | Becker, SA;Petrich, BG;Yu, B;Knight, KA;Brown, HC;Raikar, SS;Doering, CB;Spencer, HT; | Enhancing the effectiveness of ?? T cells by mRNA transfection of chimeric antigen receptors or bispecific T cell engagers | Molecular therapy oncolytics | 37387794 | ed from healthy donor blood through the Children’s Clinical Translational Discovery Core at Emory University under the core’s approved institutional review board protocol or ordered directly from AllCells. PBMCs were isolated from fresh blood using F | Full Paper |
2023 | Chervin, AS;Stone, JD;Konieczna, I;Calabrese, KM;Wang, N;Haribhai, D;Dong, F;White, MK;Rodriguez, LE;Bukofzer, GT;Ellis, PA;Cosgrove, C;Hecquet, C;Clarin, JD;Palma, JP;Reilly, EB; | ABBV-184: A novel survivin-specific TCR/CD3 bispecific T cell engager is active against both solid tumor and hematological malignancies | Molecular cancer therapeutics | 37294945 | Primary frozen viable PBMCs from AML patients and including AML blasts were purchased from Discovery Life Sciences and verified for HLA-A2 expression by staining with the HLA-A2 specific monoclonal antibody BB7.2 and detecting by flow cytometry | Full Paper |
2023 | Korntner, SH;Di Nubila, A;Gaspar, D;Zeugolis, DI; | Macromolecular crowding in animal component-free, xeno-free and foetal bovine serum media for human bone marrow mesenchymal stromal cell expansion and differentiation | Frontiers in bioengineering and biotechnology | 36949882 | ed. 2.2 Isolation and expansion of hBMSCs Fresh human whole bone marrow from the iliac crest of two different donors (donor 1: female, 22 years old; donor 2: male, 25 years old) was purchased from AllCells (United States) and hBMSCs were isolated u | Full Paper |
2023 | Dempsey, MP;Andersen, KE;Wells, BM;Taylor, MA;Cashman, CL;Conrad, LB;Kearney, CA;Conklin, MB;Via, ER;Doe, EM;Komirisetty, R;Dearborn, S;Reddy, ST;Farias-Eisner, R; | Stability Characterization of the Novel Anti-Cancer HM-10/10 HDL-Mimetic Peptide | International journal of molecular sciences | 37373203 | 82043-96OD), one plate per lot, were obtained from In Vitro ADMET Laboratories (Malden, MA, USA). The transport medium was replaced with 200 ?L of warm (37 C) Save | Full Paper |
2023 | El Ouardi, M;Tamarit, L;Vayá, I;Miranda, MA;Andreu, I; | Cellular photo(geno)toxicity of gefitinib after biotransformation | Frontiers in pharmacology | 37351506 | UPV-CSIC, Universitat Politècnica de València, Valencia, Spain 2 Unidad Mixta de Investigación UPV- IIS La Fe, Hospital Universitari i Politècnic La Fe, Valencia, Spain Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States Reviewed by | Full Paper |
2023 | Takei, S;Kinoshita, H;Jamal, M;Kumihashi, M;Yamashita, T;Tanaka, E;Kawahara, S;Abe, H;Tsutsui, K;Kimura, S; | Case report: Fatal poisoning caused by additive effects of two barbiturates | Frontiers in pharmacology | 37292155 | Medicine, Faculty of Medicine, Kagawa University, Takamatsu, Japan 2 Biodesign Inc, Tokyo, Japan 3 Kagawa Prefectural University of Health Sciences, Takamatsu, Kagawa, Japan Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States Review | Full Paper |
2023 | Li, S;Zhan, J;Wang, Y;Oduro, PK;Owusu, FB;Zhang, J;Leng, L;Li, R;Wei, S;He, J;Wang, Q; | Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone | Frontiers in pharmacology | 37234713 | vestigated. The EC50 and Rmax (maximal vasorelaxation) values were then calculated. 2.8 Cell culture and western blotting analysis Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells Biotech Co., Ltd. (Shanghai, China). The | Full Paper |
2023 | Greenstein, AE;Hunt, HJ; | The glucocorticoid receptor modulator relacorilant reverses the immunosuppressive effects of cortisol | International immunopharmacology | 37230031 | GR was assessed using a rabbit monoclonal antibody clone D8H2 from Cell Signaling Technology (Cat#3660S) as described previously [41]. CD3 (Dako, A0452), FOXP3 (eBioscience 14-4777), CD57 (ThermoFisher, MA5-12008), and PD-1 (Abcam, ab53587) were acqu | Full Paper |
2023 | Cyr, MG;Wilson, HD;Spierling, AL;Chang, J;Peng, H;Steinberger, P;Rader, C; | Concerted Antibody and Antigen Discovery by Differential Whole-cell Phage Display Selections and Multi-omic Target Deconvolution | Journal of molecular biology | 37019174 | Fresh healthy donor PBMCs were bought from AllCells and cryopreserved prior to use with 50% FBS, 10% DMSO in RPMI-1640 medium. Save | Full Paper |
2023 | Isaioglou, I;Aldehaiman, MM;Li, Y;Lahcen, AA;Rauf, S;Al-Amoodi, AS;Habiba, U;Alghamdi, A;Nozue, S;Habuchi, S;Salama, KN;Merzaban, JS; | CD34+ HSPCs-derived exosomes contain dynamic cargo and promote their migration through functional binding with the homing receptor E-selectin | Frontiers in cell and developmental biology | 37181754 | O2 concentration of 5% v/v. The cell cultures were passaged frequently to maintain the cells at 0.8 × 106 cells/ml. Primary human AML CD34+ cells from Mobilized Peripheral Blood were purchased from AllCells, while Primary Human CD34+ cells from Mobi | Full Paper |
2023 | Basu, A;Champagne, RN;Patel, NG;Nicholson, ED;Weiss, RJ; | TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth | The Journal of biological chemistry | 37061003 | in Table S4. RNA sequencing and differential gene expression analysis Total RNA extracted from wildtype and knockout cell lines was submitted for library preparation and next-generation sequencing (HudsonAlpha Discovery). Raw RNA sequencing data was | Full Paper |
2023 | Spriano, F;Aresu, L;Cascione, L;Risi, G;Arribas, AJ;Napoli, S;Forster-Gross, N;Bachmann, F;Engelhardt, M;Lane, H;Bertoni, F; | The microtubule-targeted agent lisavanbulin (BAL101553) shows anti-tumor activity in lymphoma models | American journal of cancer research | 37293172 | (TMA) (LY1001D and LY2086B, TissueArray.com) were stained for EB1 using a CE-marked immunohistochemistry Clinical Trial Assay (Discovery Life Sciences Biomarker Save | Full Paper |
2023 | Baraibar, I;García, A;Salvà, F;Ros, J;Saoudi, N;Comas, R;Castillo, G;Sanchis, M;García-Álvarez, A;Hernando, J;Capdevila, J;Castells, MR;Martí, M;Landolfi, S;Espín, E;Navalpotro, B;Guevara, J;Dopazo, C;Nuciforo, P;Vivancos, A;Tabernero, J;Élez, E; | Impact of the COVID-19 pandemic in the early-onset colorectal cancer | Translational oncology | 37031602 | Paolo Nuciforo reports personal financial interest in form of scientific consultancy role for Targos Molecular Pathology. His-institution has received research funding from Save;- Paolo Nuciforo reports personal financial interest in form of scie | Full Paper |
2023 | Pousse, L;Korfi, K;Medeiros, BC;Berrera, M;Kumpesa, N;Eckmann, J;Hutter, IK;Griesser, V;Karanikas, V;Klein, C;Amann, M; | CD25 targeting with the afucosylated human IgG1 antibody RG6292 eliminates regulatory T cells and CD25+ blasts in acute myeloid leukemia | Frontiers in oncology | 37205201 | Frozen healthy BMMC were purchased from Discovery Life Sciences. All human samples were collected under the approval of the relevant Institutional Review Board (IRB Save | Full Paper |
2023 | Kourula, S;Derksen, M;Jardi, F;Jonkers, S;van Heerden, M;Verboven, P;Theuns, V;Van Asten, S;Huybrechts, T;Kunze, A;Frazer-Mendelewska, E;Lai, KW;Overmeer, R;Roos, JL;Vries, RGJ;Boj, SF;Monshouwer, M;Pourfarzad, F;Snoeys, J; | Intestinal organoids as an in vitro platform to characterize disposition, metabolism, and safety profile of small molecules | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences | 37244450 | Intestinal organoids derived from LGR5 + adult stem cells allow for long-term culturing, more closely resemble human physiology than traditional intestinal models, like Save;- HQM Incubation Medium and co-factor N were acquired from in vitro ADME | Full Paper |
2023 | Gnanapragasam, MN;Planutis, A;Glassberg, JA;Bieker, JJ; | Identification of a genomic DNA sequence that quantitatively modulates KLF1 transcription factor expression in differentiating human hematopoietic cells | Scientific reports | 37165057 | is human GRCh37/hg19 genome browser (https://main.genome-browser.bx.psu.edu/index.html), focusing on results using CD34+ or bone marrow derived erythroid cells. Human CD34+ cells were purchased from AllCells or obtained from the Yale Cooperative Cen | Full Paper |
2023 | Li, J;Zhang, Z;Zhuang, Y;Wang, F;Cai, T; | Small RNA transcriptome analysis using parallel single-cell small RNA sequencing | Scientific reports | 37160973 | ng). PBMCs preparation Cryopreserved CD3+ Pan T cells (PB009-1F-C-5M), CD19+ B cells (PB010-P-F-C), CD14+ monocytes (PB-011-P-F-1-C), and CD56+ natural killer cells (PB012-P-F-C) were purchased from AllCells (Shanghai, China). The CD3+ Pan T cells w | Full Paper |
2023 | Ke, H;Zhang, F;Wang, J;Xiong, L;An, X;Tu, X;Chen, C;Wang, Y;Mao, B;Guo, S;Ju, C;He, X;Sun, R;Zhang, L;O'Connor, OA;Li, QX; | HX009, a novel BsAb dual targeting PD1 x CD47, demonstrates potent anti-lymphoma activity in preclinical models | Scientific reports | 37012357 | Gibco). Jurkat-CSR cells, from ImmuneOnco Biopharmaceuticals (Shanghai), were cultured in RPMI-1640 (Gibco) with 10% FBS (Gemini). Human PBMCs used in the studies were purchased from ALLCELL (https://allcells.com) where informed consent and approved | Full Paper |
2023 | Adhikary, G;Heipertz, EL;Preradovic, M;Chen, X;Xu, W;Newland, JJ;Kaur, N;Vemuri, MC;Eckert, RL; | Natural killer cells suppress human cutaneous squamous cell carcinoma cancer cell survival and tumor growth | Molecular carcinogenesis | 36994661 | Cryopreserved peripheral blood mononuclear cells (PBMCs) were obtained from AllCells. NK cells were enriched via negative selection using the Human NK Cell Save | Full Paper |
2023 | Sweeney, EE;Sekhri, P;Telaraja, D;Chen, J;Chin, SJ;Chiappinelli, KB;Sanchez, CE;Bollard, CM;Cruz, CRY;Fernandes, R; | Engineered tumor-specific T cells using immunostimulatory photothermal nanoparticles | Cytotherapy | 37278683 | ix-coated plates in Astrocyte Medium (Thermo Fisher Scientific). Cells were expanded as per the specifications provided by the supplier. Healthy donor peripheral blood leukopaks were purchased from AllCells (Alameda, CA, USA) with the corresponding h | Full Paper |
2023 | Zhang, L;Yu, L;Xu, Y;Qin, P;Shen, P;Liu, K;Fei, M;Wang, H;Cao, Y;Lu, L;Gao, W;Zhang, Z; | Demonstrating analytical similarity of a biosimilar HLX04 to bevacizumab with a panel of state-of-the-art methods and tiering of quality attributes | Analytical and bioanalytical chemistry | 37162525 | Human umbilical vein endothelial cell (HUVEC), obtained from Allcells (No. H-001F, CA, USA), was used to determine the inhibitory effects of HLX04 and CN-/EU-Avastin® on proliferation of cells expressing VEGFR. HUVEC expresses VEGFR that binds to VEG | Full Paper |
2023 | Verner, EL;Jackson, JB;Severson, E;Valkenburg, KC;Greer, AE;Riley, DR;Sausen, M;Maddox, C;McGregor, PM;Karandikar, A;Hastings, SB;Previs, RA;Reddy, VP;Jensen, TJ;Ramkissoon, SH; | Validation of the Labcorp Plasma Focus Test to Facilitate Precision Oncology Through Cell-Free DNA Genomic Profiling of Solid Tumors | The Journal of molecular diagnostics : JMD | 37068734 | Clinical samples used to evaluate the analytical performance of the Labcorp Plasma Focus test were sourced from multiple biorepositories or as part of internal sample collections (BioIVT, Chestertown, MD; Discovery Life Sciences, Huntsville, AL; Prec | Full Paper |
2023 | Ding, H;Yao, B;Ci, L;Feng, J;Ouyang, P;Chen, G;Hui, X;Zhou, D; | Enhancing the Anti-tumor Potency of a Novel Siglec-15 Antibody by Engineering its Fc-mediated Effector Functions | Journal of immunotherapy (Hagerstown, Md. : 1997) | 37103472 | Total PBMCs (AllCells) from a healthy human donor were resuspended in cell culture media (RPMI1640 + 10% FBS) at a density of 150,000 cells per well in triplicate. Save | Full Paper |
2023 | Ekiciler, A;Chen, WLK;Bo, Y;Pugliano, A;Donzelli, M;Parrott, N;Umehara, K; | Quantitative Cytochrome P450 3A4 Induction Risk Assessment Using Human Hepatocytes Complemented with Pregnane X Receptor-Activating Profiles | Drug metabolism and disposition: the biological fate of chemicals | 36460477 | on 24- or 96-well collagen I-coated plates at 0.6 or 0.06 × 10 6 cells/well, respectively, using high viability cryo-hepatocyte recovery kits (In Vitro ADMET Laboratories, LLC; Save | Full Paper |
2023 | Bell-Glenn, S;Salas, LA;Molinaro, AM;Butler, RA;Christensen, BC;Kelsey, KT;Wiencke, JK;Koestler, DC; | Calculating detection limits and uncertainty of reference-based deconvolution of whole-blood DNA methylation data | Epigenomics | 37337720 | Cells used to isolate the DNA to create the mixtures were purchased from AllCells corporation (CA, USA), StemExpress (CA, USA), and STEMCELL Technologies ( Save | Full Paper |
2023 | Banerjee, P;Diniz, WJS;Rodning, SP;Dyce, PW; | miRNA expression profiles of peripheral white blood cells from beef heifers with varying reproductive potential | Frontiers in genetics | 37234872 | The quality-checked libraries were pooled and sequenced in the NextSeq 500 using the single-end 50 bp chemistry at Discovery Life Sciences (Hudson Alpha Institute of Save;nt Bioanalyzer high-sensitivity DNA assay (Agilent, Santa Clara, CA, United S | Full Paper |
2023 | Lamothe, RC;Storlie, MD;Espinosa, DA;Rudlaff, R;Browne, P;Liu, J;Rivas, A;Devoto, A;Oki, J;Khoubyari, A;Goltsman, DSA;Lin, JL;Butterfield, CN;Brown, CT;Thomas, BC;Cost, GJ; | Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering | The CRISPR journal | 37219969 | oporation, cells were harvested for flow cytometry and genomic DNA extraction as described previously. Primary HSC culture and nucleofection Mobilized peripheral blood CD34+ cells were acquired from AllCells and cultured in StemCell StemSpan SFEM I | Full Paper |
2023 | Alexander, GE;Lin, W;Ortega, FE;Ramaiah, M;Jung, B;Ji, L;Revenkova, E;Shah, P;Croisetiere, C;Berman, JR;Eubank, L;Naik, G;Brooks, J;Mich, A;Shojaee, S;Ronaghi, N;Chawla, H;Hou, X;Liu, Q;Yakym, CAV;Moradi, PW;Halks-Miller, M;Aravanis, AM;Parpart-Li, S;Hunkapiller, N; | Analytical validation of a multi-cancer early detection test with cancer signal origin using a cell-free DNA-based targeted methylation assay | PloS one | 37058491 | Six non-cancer samples were obtained from Discovery Life Sciences (Huntsville, AL; catalog #s 220130 and 220140) and 6 cancer samples were obtained from Save;ncer) across dilution series created by spiking cfDNA from cancer samples into cfDNA deriv | Full Paper |
2023 | Li, X;Le, Y;Seo, JE;Guo, X;Li, Y;Chen, S;Mittelstaedt, RA;Moore, N;Guerrero, S;Sims, A;King, ST;Atrakchi, AH;McGovern, TJ;Davis-Bruno, KL;Keire, DA;Elespuru, RK;Heflich, RH;Mei, N; | Revisiting the mutagenicity and genotoxicity of N-nitroso propranolol in bacterial and human in vitro assays | Regulatory toxicology and pharmacology : RTP | 37210026 | National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, 72079, USA. Electronic address: xilin.li@fda.hhs.gov.++National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, 72079, | Full Paper |
2023 | Coleman, R; | Metastasis prevention with bone-targeted agents: a complex interaction between the microenvironment and tumour biology | Journal of bone and mineral metabolism | 37162605 | amplification status was blinded and followed pre-specified sample handling and standard operating methodology by a central laboratory (Targos Molecular Pathology, Save | Full Paper |
2023 | Kliver, S;Houck, ML;Perelman, PL;Totikov, A;Tomarovsky, A;Dudchenko, O;Omer, AD;Colaric, Z;Weisz, D;Aiden, EL;Chan, S;Hastie, A;Komissarov, A;Ryder, OA;Graphodatsky, A;Johnson, WE;Maldonado, JE;Pukazhenthi, BS;Marinari, PE;Wildt, DE;Koepfli, KP; | Chromosome-length genome assembly and karyotype of the endangered black-footed ferret (Mustela nigripes) | The Journal of heredity | 37249392 | The black-footed ferret (Mustela nigripes) narrowly avoided extinction to become an oft-cited example of the benefits of intensive management, research, and collaboration Save | Full Paper |
2023 | Martha, L;Nakata, A;Furuya, S;Liu, W;Zhang, X;Mizoi, K;Ogihara, T; | Transporter and metabolic enzyme-mediated intra-enteric circulation of SN-38, an active metabolite of irinotecan: A new concept | Biochemical and biophysical research communications | 37148742 | sages 72-83 were used. Human cryopreserved suspension enterocytes (Lot. HE3072, male, 55 years old, Caucasian) were purchased from In Vitro ADMET Laboratories (MD, USA). All other reagents and solvents were commercial products of special grade | Full Paper |
2023 | He, S;Liu, J;Hu, L;Zhan, Y;Tong, H;Zhu, H;Guo, H;Sun, H;Liu, M; | Design, synthesis, biological evaluation and molecular docking studies of quercetin-linker-H2S donor conjugates for the treatment of diabetes and wound healing | Chemistry & biodiversity | 37329234 | All cell culture materials were obtained from Gibco. HepG2 cells were cryopreserved in our laboratory and used after resuscitation. HUVECs cells were purchased from Shanghai Allcells Co., Ltd | Full Paper |
2023 | Thompson, EN;Carlino, MJ;Scanlon, VM;Grimes, HL;Krause, DS; | Assay optimization for the objective quantification of human multilineage colony-forming units | Experimental hematology | 37271449 | Cells were derived commercially (AllCells, Alameda, CA) or were derived from healthy donors who were already donating cells for allogeneic transplantation provided Save | Full Paper |
2023 | Goda, C;Kolovich, S;Rudich, A;Karunasiri, M;Kulkarni, R;Rajgolikar, G;Neidemire-Colley, L;Singh, S;Sircar, A;Ranganathan, P;Garzon, R;Sehgal, L;Dorrance, AM; | Epidermal growth factor-like 7 is a novel therapeutic target in mantle cell lymphoma | Experimental hematology | 37209901 | Full Paper | |
2023 | Yi, PC;Zhuo, L;Lin, J;Chang, C;Goddard, A;Yoon, OK; | Impact of delayed PBMC processing on functional and genomic assays | Journal of immunological methods | 37353001 | An additional five whole blood samples from healthy adults were sourced from AllCells (Alameda, CA, USA), all of whom provided informed consent, under their IRB- Save | Full Paper |
2023 | Chen, Z;Javed, N;Moore, M;Wu, J;Sun, G;Vinyard, M;Collins, A;Pinello, L;Najm, FJ;Bernstein, BE; | Integrative dissection of gene regulatory elements at base resolution | Cell genomics | 37388913 | us Biologicals Cat#NB100-1800 TAL1 antibody SantaCruz Biotech Cat#sc-393287 Cell lines and primary cells Jurkat cell line, Clone E6.1 ATCC Cat#TIB152, RRID:CVCL_0367 CD4+ T cells AllCells N/A ------------------------- Chemical | Full Paper |
2023 | Kessler, ER;Callihan, E;Hu, J;Eule, C;Srivastava, G;Kemme, DJ;Iruku, P;Rana, V;Moore, J;Schuster, SR;Amirault, M;Flaig, TW;Lam, ET; | A Phase I/II Clinical Trial of Pembrolizumab and Cabozantinib in Metastatic Renal Cell Carcinoma | Cancer research communications | 37377613 | Full Paper | |
2023 | Farabaugh, CS;Doak, S;Roy, S;Elespuru, R; | In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B | Frontiers in toxicology | 37180488 | Urbana?Champaign, Champaign, United States Khaled Habas, University of Bradford, United Kingdom *Correspondence: Rosalie Elespuru, rosalie.elespuru@dls.com † Present address: Rosalie Elespuru, Discovery Life Sciences, Columbia, MD, United States | Full Paper |
2023 | Baro-Sastre, B;Kim, CY;Lin, C;Kongsomboonvech, AK;Tetard, M;Salinas, ND;Tolia, NH;Egan, ES; | Plasmodium falciparum exploits CD44 as a co-receptor for erythrocyte invasion | bioRxiv : the preprint server for biology | 37090581 | Generation of cRBCs was performed essentially as previously described 37. Briefly, bone marrow primary human CD34+ HSPCs (Stem Cell Technologies; AllCells) were cultured in PIMDM composed of Iscove Basal Medium (Sigma) with 4 mM L-Glutamine, 330 µg/m | Full Paper |
2023 | Okano, F;Saito, T;Minamida, Y;Kobayashi, S;Ido, T;Miyauchi, Y;Wasai, U;Akazawa, D;Kume, M;Ishibashi, M;Jiang, K;Aicher, A;Heeschen, C;Yonehara, T; | Identification of Membrane-expressed CAPRIN-1 as a Novel and Universal Cancer Target, and Generation of a Therapeutic Anti-CAPRIN-1 Antibody TRK-950 | Cancer research communications | 37082579 | ng formula based on the Cr-51 dose released from the target cells: The ADCC activity of TRK-950 via human and monkey PBMCs was conducted at Translational Drug Development (TD2). We used human PBMCs (AllCells, catalog no. PB003F) and Cynomolgus Monke | Full Paper |
2023 | Montalban-Bravo, G;Ma, F;Thongon, N;Yang, H;Gomez, IG;Rodriguez-Sevilla, JJ;Adema, V;Wildeman, B;Lockyer, P;Kim, YJ;Tanaka, T;Darbaniyan, F;Pancholy, S;Zhang, G;Al-Atrash, G;Dwyer, K;Takahashi, K;Garcia-Manero, G;Kantarjian, H;Colla, S; | Targeting MCL1-driven anti-apoptotic pathways to overcome hypomethylating agent resistance in RAS -mutated chronic myelomonocytic leukemia | bioRxiv : the preprint server for biology | 37066354 | RAS pathway mutations, which are present in 30% of patients with chronic myelomonocytic leukemia (CMML) at diagnosis, confer a high risk of resistance to and Save | Full Paper |
2023 | Dillon, LW;Higgins, J;Nasif, H;Othus, M;Beppu, L;Smith, TH;Schmidt, E;Valentine, CC;Salk, JJ;Wood, BL;Erba, HP;Radich, JP;Hourigan, CS; | Quantification of measurable residual disease using duplex sequencing in adults with acute myeloid leukemia | medRxiv : the preprint server for health sciences | 37034683 | The presence of measurable residual disease (MRD) is strongly associated with treatment outcomes in acute myeloid leukemia (AML). Despite the correlation with clinical Save | Full Paper |
2023 | Flümann, R;Hansen, J;Pelzer, BW;Nieper, P;Lohmann, T;Kisis, I;Riet, T;Kohlhas, V;Nguyen, PH;Peifer, M;Abedpour, N;Bosco, G;Thomas, RK;Kochanek, M;Knüfer, J;Jonigkeit, L;Beleggia, F;Holzem, A;Büttner, R;Lohneis, P;Meinel, J;Ortmann, M;Persigehl, T;Hallek, M;Calado, DP;Chmielewski, M;Klein, S;Göthert, JR;Chapuy, B;Zevnik, B;Wunderlich, FT;von Tresckow, B;Jachimowicz, RD;Melnick, AM;Reinhardt, HC;Knittel, G; | Distinct Genetically Determined Origins of Myd88/BCL2-Driven Aggressive Lymphoma Rationalize Targeted Therapeutic Intervention Strategies | Blood cancer discovery | 36346827 | Roche, Cofounder and CSO of Targos MP Inc. (until May 2021), Gnothis Inc/Stockholm (Member of Board of Directors and Coowner). D.P. Calado reports other support from Cancer Research UK (CC2078), Wellcome Trust (CC2078), and UK Medical Research Counci | Full Paper |
2023 | Mark W., R;Joanne, W;Mary Jo, K;Melissa E., M;John V., L;Peter, F; | Sleep Disturbances and Chronic Pain as Potential Indicators for Therapeutic Intervention in Subjects with Post-Traumatic Stress Disorder | Journal of Depression and Anxiety Disorders | cruited to an age and gender-matched case-control study (N = 78; control n = 39; PTSD n = 39). Participants were recruited in the US between January and June 2019 by PrecisionMed (California, US) and Discovery Life Sciences (California, US). A compre | Full Paper | |
2023 | Buckley, D;Tew, B;Gooden, G;Salhia, B; | Whole genome bisulfite sequencing of tumor DNA and matching cfDNA in relapsed pediatric cancer | Research Square | from Conversant Bio (Table 1). Twelve additional cfDNA healthy controls from adults were included for model building in cfDNA, detailed below (Conversant Bio). Patients Save | Full Paper | |
2023 | Ridzuan, A;Sebastian, A;Noorazman, A;Kannan, T;Syed, N;Nurul, A; | Interleukin-17A enhances osteogenic differentiation by activating ERK/MAPK in stem cells derived from human exfoliated deciduous teeth | Biomedical Research and Therapy | the participation of the MAPK signaling pathway in SHED osteogenic differentiation. MATERIALS AND METHODS CELL CULTURE AND OSTEOGENIC DIFFERENTIATION IN VITRO The SHED cell line, obtained from ALLCELLS (Alameda, CA, USA), was derived from huma | Full Paper | |
2023 | Pillsbury, C;Dougan, J;Rabe, J;Fonseca, J;Zhou, C;Evans, A;Abukharma, H;Ichoku, O;Gonzalez-Flamenco, G;Park, S;Aljudi, A;DeRyckere, D;Castellino, S;Rafiq, S;Langermann, S;Liu, L;Henry, C;Porter, C; | Siglec-15 promotes evasion of adaptive immunity in B-cell acute lymphoblastic leukemia | Cancer Research Communications | All samples were acquired after written informed consent was provided. Residual, fresh bone marrow aspirate and peripheral blood samples from pediatric B-ALL patients were analyzed for Sig15 expression by flow cytometry under protocol (IRB#96145). Bo | Full Paper | |
2023 | Kessler, E;Callihan, E;Hu, J;Eule, C;Srivastava, G;Kemme, D;Iruku, P;Rana, V;Moore, J;Schuster, S;Amirault, M;Flaig, T;Lam, E; | Data from A Phase I/II Clinical Trial of Pembrolizumab and Cabozantinib in Metastatic Renal Cell Carcinoma | American Association for Cancer Research | PD-L1 testing was performed on archival tumor tissues by Discovery Life Sciences (formerly QualTek Molecular Laboratories) using a previously validated IHC assay for Save | Full Paper | |
2023 | Sharma, P;Zhang, X;Ly, K;Kim, J;Wan, Q;Kim, J;Lou, M;Kain, L;Teyton, L;Winau, F; | Hyperglycosylation of prosaposin in tumor DCs promotes immune escape in cancer | bioRxiv | Dissociated whole tumor samples were purchased from Discovery Life Sciences (USA). Melanoma, myeloid, and CD8 T cells were FACS-sorted as described above. Melanoma cells were ?-irradiated (10,000 rad), and induction of apoptosis was | Full Paper | |
2023 | Tierney, B;Foox, J;Ryon, K;Butler, D;Damle, N;Young, B;Mozsary, C;Babler, K;Yin, X;Carattini, Y;Andrews, D;Solle, N;Kumar, N;Shukla, B;Vidovic, D;Currall, B;Williams, S;Schürer, S;Stevenson, M;Amirali, A;Beaver, C;Kobetz, E;Boone, M;Reding, B;Laine, J;Comerford, S;Lamar, W;Tallon, J;Hirschberg, J;Proszynski, J;Sharkey, M;Church, G;Grills, G;Solo-Gabriele, H;Mason, C; | Geospatially-resolved public-health surveillance via wastewater sequencing | medRxiv | 550 HudsonAlpha Discovery (Huntsville, AL) performed all sequencing library preparation on the 551 generated nucleic acids. The nucleic acid samples underwent Save | Full Paper | |
2023 | Zhang, X;Meng, F;Lyu, W;He, J;Wei, R;Du, Z;Zhang, C;Guan, Y;Huang, X;Lyu, G;Tian, X;Zhang, L;Tao, W; | Histone lactylation antagonizes senescence and skeletal muscle aging via facilitating gene expression reprogramming | bioRxiv | IMR90 cells were purchased from the American Type Culture Collection (ATCC), HUVECs were purchased from AllCells, and MEF cells were isolated from 12.5-14- day-old embryos of C57BL/6 mice | Full Paper | |
2023 | Sorensen, JT; | Classification of a novel subgroup of shared hepatitis B virus core and e antigen epitopes and their antibody reactivity in clinical immunoassay formats | Thesis | Protocols for BSL-2 ELISA are summarized in section 5.3.1. HBV positive samples were all purchased from Discovery Life Sciences Biological Sample Repository | Full Paper | |
2023 | Najia, MA; | Transposable elements and the regulatory logic of hematopoietic differentiation | Thesis | umbilical cord blood cells (AllCells) were thawed from liquid nitrogen storage via CD34+ umbilical cord blood cells (AllCells) were thawed from liquid nitrogen storage and Save | Full Paper | |
2023 | Schejtman Borisonik, A; | Lentiviral gene therapy for p47-deficient Chronic Granulomatous disease | Thesis | CD34+ cells used in the experiments were frozen vials of previously isolated HD CD34+ cells from a G-CSF mobilised apheresis (ALLCells) using the CliniMACS prodigy | Full Paper | |
2023 | Kortchak, S; | Development of Programmed Cell Death Ligand Derived Peptides for the Treatment of Autoimmune Diseases | Thesis | Cell Culture. Jurkat NFAT-PD-1 reporter cells (BPS Biosciences, San Diego, CA) were maintained in Growth Medium 2A (BPS Biosciences, San Diego, CA) medium. Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells and maintained in RPMI | Full Paper | |
2023 | Maughon Jr, TS; | Metabolic Profiling of Mesenchymal Stromal Cells for Critical Quality Attribute Identification | Thesis | at three densities (10,000, 5,000 and 2,000 cells/well) in a 96 well plate and cultured for 24 hours. PBMCs (AllCells, Alameda CA) were thawed in Roswell Park Memorial Institute (RPMI) media (RPMI, 20% FBS, 2mM L-glutamine, 50 U/mL penicillin, 50 ?g/ | Full Paper | |
2023 | Han, L;Dong, L;Leung, K;Zhao, Z;Li, Y;Gao, L;Chen, Z;Xue, J;Qing, Y;Li, W;Pokharel, SP;Gao, M;Chen, M;Shen, C;Tan, B;Small, A;Wang, K;Zhang, Z;Qin, X;Yang, L;Wunderlich, M;Zhang, B;Mulloy, JC;Marcucci, G;Chen, CW;Wei, M;Su, R;Chen, J;Deng, X; | METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolism | Cell stem cell | 36608679 | ColonyGEL-Mouse Base Medium Reachbio Cat# 1201 ColonyGEL -Human Base Medium Reachbio Cat# 1101 | Full Paper |
2023 | Le Floc'h, A;Nagashima, K;Birchard, D;Scott, G;Ben, LH;Ajithdoss, D;Gayvert, K;Romero Hernandez, A;Herbin, O;Tay, A;Farrales, P;Korgaonkar, CK;Pan, H;Shah, S;Kamat, V;Chatterjee, I;Popke, J;Oyejide, A;Lim, WK;Kim, JH;Huang, T;Franklin, M;Olson, W;Norton, T;Perlee, L;Yancopoulos, GD;Murphy, AJ;Sleeman, MA;Orengo, JM; | Blocking common γ chain cytokine signaling ameliorates T cell-mediated pathogenesis in disease models | Science translational medicine | 36630481 | To induce GVHD, we injected immunodeficient NSG (Jackson Laboratory, 005557) female mice (8 weeks old) retro-orbitally with 10 7 human PBMCs (ReachBio, 0500-401). Mice were Save | Full Paper |
2023 | Wang, H;Georgakopoulou, A;Zhang, W;Kim, J;Gil, S;Ehrhardt, A;Lieber, A; | HDAd6/35++ - A new helper-dependent adenovirus vector platform for in vivo transduction of hematopoietic stem cells | Molecular therapy. Methods & clinical development | 37081854 | Lineage-negative (Lin-) cells were isolated by depletion of lineage-committed cells in BM MNCs using the mouse lineage cell depletion kit (Miltenyi Biotec) according to the manufacturer’s instructions. Colony-forming unit assays were performed using | Full Paper |
2023 | Belcher, JD;Nataraja, S;Abdulla, F;Zhang, P;Chen, C;Nguyen, J;Ruan, C;Singh, M;Demes, S;Olson, L;Stickens, D;Stanwix, J;Clarke, E;Huang, Y;Biddle, M;Vercellotti, GM; | The BACH1 inhibitor ASP8731 inhibits inflammation and vaso-occlusion and induces fetal hemoglobin in sickle cell disease | Frontiers in medicine | 37144034 | Department of Medicine, Division of Hematology, Oncology and Transplantation, University of Minnesota, Minneapolis, MN, United States.++Mitobridge Inc., Cambridge, MA, United States.++Department of Medicine, Division of Hematology, Oncology and Trans | Full Paper |
2023 | Wu, T;Pelus, LM;Plett, PA;Sampson, CH;Chua, HL;Fisher, A;Feng, H;Liu, L;Li, H;Ortiz, M;Chittajallu, S;Luo, Q;Bhatwadekar, AD;Meyer, TB;Zhang, X;Zhou, D;Fischer, KD;McKinzie, DL;Miller, SJ;Orschell, CM; | Further Characterization of Multi-Organ DEARE and Protection by 16,16 Dimethyl Prostaglandin E2 in a Mouse Model of the Hematopoietic Acute Radiation Syndrome | Radiation research | 37014943 | Total nucleated BM cells from non-irradiated mice were plated at 2 × 104 per dish in ColonyGEL 1202 Mouse Complete Medium (Reach Bio, Seattle, WA) | Full Paper |
2023 | Borot, F;Humbert, O;Newby, GA;Fields, E;Kohli, S;Radtke, S;Laszlo, GS;Mayuranathan, T;Ali, AM;Weiss, MJ;Yen, JS;Walter, RB;Liu, DR;Mukherjee, S;Kiem, HP; | Multiplex Base Editing to Protect from CD33-Directed Therapy: Implications for Immune and Gene Therapy | bioRxiv : the preprint server for biology | 36865281 | Cord blood CD34+ 526 cells were 527 additionally cultured with UM171 0.35 nM (Xcessbio). In colony-forming cell assays, 1000-1200 528 sorted cells were seeded into 3.5 ml ColonyGEL 1402 (ReachBio) | Full Paper |
2023 | Vujovic, A;de Rooij, L;Chahi, AK;Chen, HT;Yee, BA;Loganathan, SK;Liu, L;Chan, DCH;Tajik, A;Tsao, E;Moreira, S;Joshi, P;Xu, J;Wong, N;Balde, Z;Jahangiri, S;Zandi, S;Aigner, S;Dick, JE;Minden, MD;Schramek, D;Yeo, GW;Hope, KJ; | In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target | Blood cancer discovery | 36763002 | Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio cat. no. 1102), supplemented with 1.1 μmol/L DHTS (MilliporeSigma; cat. no. D0947-10MG) or equivalent DMSO (Fish | Full Paper |
2023 | Zhang, Z;Zhou, K;Han, L;Small, A;Xue, J;Huang, H;Weng, H;Su, R;Tan, B;Shen, C;Li, W;Zhao, Z;Qing, Y;Qin, X;Wang, K;Leung, K;Boldin, M;Chen, C;Ann, D;Qian, Z;Deng, X;Chen, J;Chen, Z; | RNA m6A reader YTHDF2 facilitates precursor miR-126 maturation to promote acute myeloid leukemia progression | Genes & Diseases | ColonyGel 1201-mouse base medium (1201, Reachbio) were used for seeding cells at 20, 000 per 35 mm dish. Murine cytokines and reagents were supplemented as follows: 10 ng/ Save | Full Paper | |
2023 | Davies, J;Prieto, EM;Guelcher, S; | Characterization of Macrophage Behavior in the Human Immunoresponse and Bone Remodeling | cdn.vanderbilt.edu | We cultured primary human monocytes (Reachbio, Seattle, WA) in Corning T25 flasks with 10 mL of modified Dulbecco’s modified Eagle medium (DMEM) media and split them every 9 days. We changed media on Day 1, 5, and 7, according to Table 1. Penicillin | Full Paper | |
2023 | Vujovic, A; | Investigating RNA-Binding Protein Dependencies in Hematopoietic and Acute Myeloid Leukemic Stem Cells | Thesis | Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio Cat# 1102), supplemented with 1.1μM DHTS (MilliporeSigma Cat# D0947-10MG) or equivalent DMSO (Fisher Scientific | Full Paper | |
2022 | Somarathna, M;Hwang, PT;Millican, RC;Alexander, GC;Isayeva-Waldrop, T;Sherwood, JA;Brott, BC;Falzon, I;Northrup, H;Shiu, YT;Stubben, CJ;Totenhagen, J;Jun, HW;Lee, T; | Nitric oxide releasing nanomatrix gel treatment inhibits venous intimal hyperplasia and improves vascular remodeling in a rodent arteriovenous fistula | Biomaterials | 34836683 | RNA isolation and sequencing were performed by Discovery Life Sciences (Huntsville, AL). Total RNA was isolated using standard Discovery Life Sciences protocols. All samples underwent RNA quality control assessment using a Fragment Analyzer (Agilen | Full Paper |
2022 | Choi, DY;Park, JN;Paek, SH;Choi, SC;Paek, SH; | Detecting early-stage malignant melanoma using a calcium switch-enriched exosome subpopulation containing tumor markers as a sample | Biosensors & bioelectronics | 34847362 | Clinical samples of human sera from healthy donors and malignant melanoma patients were obtained from Discovery Life Sciences (DLS; Los Osos, CA, USA). Other materials are listed and described about the sources in details in Supplementary Informati | Full Paper |
2022 | Bormann Chung, C;Lee, J;Barritault, M;Bringuier, PP;Xu, Z;Huang, WY;Beharry, A;Castillo, J;Christiansen, J;Lin, JC;Sheffield, BS; | Evaluating Targeted Next-Generation Sequencing Assays and Reference Materials for NTRK Fusion Detection | The Journal of molecular diagnostics : JMD | 34656759 | The 30 FFPE NTRK fusion-negative tumor samples (confirmed as NTRK fusion negative by an NGS laboratory-developed test) tested in the specificity analyses for AFL, TSO500, and OFA were purchased from Indivumed GmbH (Hamburg, Germany) and Discovery Lif | Full Paper |
2022 | Freeman, J;Conklin, J; | Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material | Journal of virological methods | 34915088 | WHO 20/136 is standard reference material for SARS-CoV-2 serology assays. Standardization of serology assays that target the same antigen and class of immunoglobulin will enable comparison of results between studies that use various lab-developed a | Full Paper |
2022 | Ganan-Gomez, I;Yang, H;Ma, F;Montalban-Bravo, G;Thongon, N;Marchica, V;Richard-Carpentier, G;Chien, K;Manyam, G;Wang, F;Alfonso, A;Chen, S;Class, C;Kanagal-Shamanna, R;Ingram, JP;Ogoti, Y;Rose, A;Loghavi, S;Lockyer, P;Cambo, B;Muftuoglu, M;Schneider, S;Adema, V;McLellan, M;Garza, J;Marchesini, M;Giuliani, N;Pellegrini, M;Wang, J;Walker, J;Li, Z;Takahashi, K;Leverson, JD;Bueso-Ramos, C;Andreeff, M;Clise-Dwyer, K;Garcia-Manero, G;Colla, S; | Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy | Nature medicine | 35241842 | simultaneously received the cytidine deaminase inhibitor E7727 (cedazuridine) to increase HMA bioavailability. BM samples from 21 HDs (median age, 53 years; range, 49-66 years) were obtained from AllCells or from MD Anderson’s Department of Stem Cel | Full Paper |
2022 | Lebedin, M;Foglierini, M;Khorkova, S;Vázquez García, C;Ratswohl, C;Davydov, AN;Turchaninova, MA;Daubenberger, C;Chudakov, DM;Lanzavecchia, A;de la Rosa, K; | Different classes of genomic inserts contribute to human antibody diversity | Proceedings of the National Academy of Sciences of the United States of America | 36037353 | 42), and ethical review boards at Ifakara Health Institute and the University of Lausanne, and all volunteers provided informed consent before blood donation. Bone marrow specimens were obtained from AllCells. Extraction of Human PBMCs. Blood sample | Full Paper |
2022 | Simmons, SK;Lithwick-Yanai, G;Adiconis, X;Oberstrass, F;Iremadze, N;Geiger-Schuller, K;Thakore, PI;Frangieh, CJ;Barad, O;Almogy, G;Rozenblatt-Rosen, O;Regev, A;Lipson, D;Levin, JZ; | Mostly natural sequencing-by-synthesis for scRNA-seq using Ultima sequencing | Nature biotechnology | 36109685 | We purchased nine cryopreserved human PMBC samples (AllCells). We thawed PBMC vials in a 37 °C water bath for ~2 min. A quick counting revealed high viability (90%) in all samples. We added 1 ml RPMI1640 (Thermo Fisher Scientific, 11875093) with 10% | Full Paper |
2022 | Shy, BR;Vykunta, VS;Ha, A;Talbot, A;Roth, TL;Nguyen, DN;Pfeifer, WG;Chen, YY;Blaeschke, F;Shifrut, E;Vedova, S;Mamedov, MR;Chung, JJ;Li, H;Yu, R;Wu, D;Wolf, J;Martin, TG;Castro, CE;Ye, L;Esensten, JH;Eyquem, J;Marson, A; | High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails | Nature biotechnology | 36008610 | Primary adult blood cells were obtained from healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc. or Allcells Inc, as a Trima residual from Vitalant, or from fresh whole blood under a protocol approved by the UCSF C | Full Paper |
2022 | Zhang, B;Srivastava, A;Mimitou, E;Stuart, T;Raimondi, I;Hao, Y;Smibert, P;Satija, R; | Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro | Nature biotechnology | 35332340 | available on the protocols.io platform, here: https://www.protocols.io/private/57E45A034D5311ECA43B0A58A9FEAC02. PBMC acquisition and processing Cryopreserved healthy donor PBMCs were purchased from AllCells. After thawing into DMEM with 10% FBS, th | Full Paper |
2022 | Fiskin, E;Lareau, CA;Ludwig, LS;Eraslan, G;Liu, F;Ring, AM;Xavier, RJ;Regev, A; | Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC | Nature biotechnology | 34675424 | Thermo Fisher Scientific), resuspended in DMEM containing 10% FBS, centrifuged, washed with PBS and resuspended in FC buffer (above). For PBMC and CD8 T-cell experiments, cryopreserved PBMCs or CD8 T cells (AllCells) were thawed, washed in PBS and re | Full Paper |
2022 | Omer-Javed, A;Pedrazzani, G;Albano, L;Ghaus, S;Latroche, C;Manzi, M;Ferrari, S;Fiumara, M;Jacob, A;Vavassori, V;Nonis, A;Canarutto, D;Naldini, L; | Mobilization-based chemotherapy-free engraftment of gene-edited human hematopoietic stem cells | Cell | 35617958 | V4XP-3032 TaqMan Gene Expression Assay ThermoFisher Scientifi Cat# 4331182 ------------------------- Experimental models: Cell lines ------------------------- Human: G-CSF mPB CD34+ HSPCs AllCells N/A Human: G-CSF/Mozobil mPB CD34+ HS | Full Paper |
2022 | Battaglia, S;Dong, K;Wu, J;Chen, Z;Najm, FJ;Zhang, Y;Moore, MM;Hecht, V;Shoresh, N;Bernstein, BE; | Long-range phasing of dynamic, tissue-specific and allele-specific regulatory elements | Nature genetics | 36195755 | Epigenomic maps identify gene regulatory elements by their chromatin state. However, prevailing short-read sequencing methods cannot effectively distinguish alleles, evaluate the Save | Full Paper |
2022 | Bottomly, D;Long, N;Schultz, AR;Kurtz, SE;Tognon, CE;Johnson, K;Abel, M;Agarwal, A;Avaylon, S;Benton, E;Blucher, A;Borate, U;Braun, TP;Brown, J;Bryant, J;Burke, R;Carlos, A;Chang, BH;Cho, HJ;Christy, S;Coblentz, C;Cohen, AM;d'Almeida, A;Cook, R;Danilov, A;Dao, KT;Degnin, M;Dibb, J;Eide, CA;English, I;Hagler, S;Harrelson, H;Henson, R;Ho, H;Joshi, SK;Junio, B;Kaempf, A;Kosaka, Y;Laderas, T;Lawhead, M;Lee, H;Leonard, JT;Lin, C;Lind, EF;Liu, SQ;Lo, P;Loriaux, MM;Luty, S;Maxson, JE;Macey, T;Martinez, J;Minnier, J;Monteblanco, A;Mori, M;Morrow, Q;Nelson, D;Ramsdill, J;Rofelty, A;Rogers, A;Romine, KA;Ryabinin, P;Saultz, JN;Sampson, DA;Savage, SL;Schuff, R;Searles, R;Smith, RL;Spurgeon, SE;Sweeney, T;Swords, RT;Thapa, A;Thiel-Klare, K;Traer, E;Wagner, J;Wilmot, B;Wolf, J;Wu, G;Yates, A;Zhang, H;Cogle, CR;Collins, RH;Deininger, MW;Hourigan, CS;Jordan, CT;Lin, TL;Martinez, ME;Pallapati, RR;Pollyea, DA;Pomicter, AD;Watts, JM;Weir, SJ;Druker, BJ;McWeeney, SK;Tyner, JW; | Integrative analysis of drug response and clinical outcome in acute myeloid leukemia | Cancer cell | 35868306 | Acute myeloid leukemia (AML) is a cancer of myeloid-lineage cells with limited therapeutic options. We previously combined ex vivo drug sensitivity with genomic, transcriptomic, and Save | Full Paper |
2022 | Ma, H;Guo, Y;Tang, H;Tseng, CK;Wang, L;Zong, H;Wang, Z;He, Y;Chang, Y;Wang, S;Huang, H;Ke, Y;Yuan, Y;Wu, M;Zhang, Y;Drelich, A;Kempaiah, KR;Peng, BH;Wang, A;Yang, K;Yin, H;Liu, J;Yue, Y;Xu, W;Zhu, S;Ji, T;Zhang, X;Wang, Z;Li, G;Liu, G;Song, J;Mu, L;Xiang, Z;Song, Z;Chen, H;Bian, Y;Zhang, B;Chen, H;Zhang, J;Liao, Y;Zhang, L;Yang, L;Chen, Y;Gilly, J;Xiao, X;Han, L;Jiang, H;Xie, Y;Zhou, Q;Zhu, J; | Broad ultra-potent neutralization of SARS-CoV-2 variants by monoclonal antibodies specific to the tip of RBD | Cell discovery | 35169121 | In ADCP experiment, CD14 + monocytes (Allcells) were cultured and differentiated for 7 days to obtain macrophage cells. Macrophages were labeled with violet dye (ThermoFisher), Save | Full Paper |
2022 | Ferrari, S;Jacob, A;Cesana, D;Laugel, M;Beretta, S;Varesi, A;Unali, G;Conti, A;Canarutto, D;Albano, L;Calabria, A;Vavassori, V;Cipriani, C;Castiello, MC;Esposito, S;Brombin, C;Cugnata, F;Adjali, O;Ayuso, E;Merelli, I;Villa, A;Di Micco, R;Kajaste-Rudnitski, A;Montini, E;Penaud-Budloo, M;Naldini, L; | Choice of template delivery mitigates the genotoxic risk and adverse impact of editing in human hematopoietic stem cells | Cell stem cell | 36206730 | tain Kit Thermo Fisher Cat# L34957 7-aminoactinomycin D Sigma Aldrich SML1633; CAS: 7240-37-1 ------------------------- Biological samples ------------------------- Mobilized Leukopak AllCells N/A ------------------------- Chemica | Full Paper |
2022 | Chan, S;Belmar, N;Ho, S;Rogers, B;Stickler, M;Graham, M;Lee, E;Tran, N;Zhang, D;Gupta, P;Sho, M;MacDonough, T;Woolley, A;Kim, H;Zhang, H;Liu, W;Zheng, P;Dezso, Z;Halliwill, K;Ceccarelli, M;Rhodes, S;Thakur, A;Forsyth, CM;Xiong, M;Tan, SS;Iyer, R;Lake, M;Digiammarino, E;Zhou, L;Bigelow, L;Longenecker, K;Judge, RA;Liu, C;Trumble, M;Remis, JP;Fox, M;Cairns, B;Akamatsu, Y;Hollenbaugh, D;Harding, F;Alvarez, HM; | An anti-PD-1-GITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapy | Nature cancer | 35256819 | Human allogeneic PBMCs (AllCells) were used to purify negatively selected populations of T cells (catalog no. 19051, Stemcell) and CD11b monocytes (catalog no. 19058, Stemcell). Save | Full Paper |
2022 | Dey, M;Kim, M;Dogan, M;Nagamine, M;Kozhaya, L;Celik, N;Unutmaz, D;Ozbolat, I; | Chemotherapeutics and CAR‐T Cell‐Based Immunotherapeutics Screening on a 3D Bioprinted Vascularized Breast Tumor Model | Advanced Functional Materials | Leukopaks from healthy adults were purchased from ALLCells (Alameda, CA) in deidentified form and processed to isolate Peripheral Blood Mononuclear Cells (PBMCs) using Ficoll-paque plus (GE Healthcare). CD8+ T cells were purified from PBMCs using Dyn | Full Paper | |
2022 | Elagib, KE;Brock, A;Clementelli, CM;Mosoyan, G;Delehanty, LL;Sahu, RK;Pacheco-Benichou, A;Fruit, C;Besson, T;Morris, SW;Eto, K;Jobaliya, C;French, DL;Gadue, P;Singh, S;Shi, X;Qin, F;Cornelison, R;Li, H;Iancu-Rubin, C;Goldfarb, AN; | Relieving DYRK1A repression of MKL1 confers an adult-like phenotype to human infantile megakaryocytes | The Journal of clinical investigation | 35925681 | alculator/hypergeometric.aspx). A P value less than 0.05 was considered significant. Study approval. CD34+ cells purchased from the Fred Hutchinson Cancer Research Center (Seattle, Washington, USA), AllCells (Alameda, California, USA), and StemCell | Full Paper |
2022 | Brady, JM;Phelps, M;MacDonald, SW;Lam, EC;Nitido, A;Parsons, D;Boutros, CL;Deal, CE;Garcia-Beltran, WF;Tanno, S;Natarajan, H;Ackerman, ME;Vrbanac, VD;Balazs, AB; | Antibody-mediated prevention of vaginal HIV transmission is dictated by IgG subclass in humanized mice | Science translational medicine | 35895834 | To generate huPBMC-engrafted NSG mice, frozen huPBMCs (AllCells) were thawed and expanded in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum ( Save | Full Paper |
2022 | Jennings, D;Huntwork-Rodriguez, S;Henry, AG;Sasaki, JC;Meisner, R;Diaz, D;Solanoy, H;Wang, X;Negrou, E;Bondar, VV;Ghosh, R;Maloney, MT;Propson, NE;Zhu, Y;Maciuca, RD;Harris, L;Kay, A;LeWitt, P;King, TA;Kern, D;Ellenbogen, A;Goodman, I;Siderowf, A;Aldred, J;Omidvar, O;Masoud, ST;Davis, SS;Arguello, A;Estrada, AA;de Vicente, J;Sweeney, ZK;Astarita, G;Borin, MT;Wong, BK;Wong, H;Nguyen, H;Scearce-Levie, K;Ho, C;Troyer, MD; | Preclinical and clinical evaluation of the LRRK2 inhibitor DNL201 for Parkinson's disease | Science translational medicine | 35675433 | 2017 {study IDs: SAN-BB-01 [approved by Quorum Review independent review board (IRB)], PLMDEN201609v1 (approved by New England IRB), and US-IRB-13-2016.1 (approved by E&I Review Services)}. PBMCs from two donors for pS935 LRRK2 and pT73 Rab10 dose-re | Full Paper |
2022 | Barreyro, L;Sampson, AM;Ishikawa, C;Hueneman, KM;Choi, K;Pujato, MA;Chutipongtanate, S;Wyder, M;Haffey, WD;O'Brien, E;Wunderlich, M;Ramesh, V;Kolb, EM;Meydan, C;Neelamraju, Y;Bolanos, LC;Christie, S;Smith, MA;Niederkorn, M;Muto, T;Kesari, S;Garrett-Bakelman, FE;Bartholdy, B;Will, B;Weirauch, MT;Mulloy, JC;Gul, Z;Medlin, S;Kovall, RA;Melnick, AM;Perentesis, JP;Greis, KD;Nurmemmedov, E;Seibel, WL;Starczynowski, DT; | Blocking UBE2N abrogates oncogenic immune signaling in acute myeloid leukemia | Science translational medicine | 35263148 | BM samples directly from patients and sequenced. Healthy BM specimens were obtained from ALLCells Inc. Human CD34+ umbilical cord blood and adult BM-derived mononuclear cells were obtained from the Translational Research Development Support Laborator | Full Paper |
2022 | Sandberg, ML;Wang, X;Martin, AD;Nampe, DP;Gabrelow, GB;Li, CZ;McElvain, ME;Lee, WH;Shafaattalab, S;Martire, S;Fisher, FA;Ando, Y;Liu, E;Ju, D;Wong, LM;Xu, H;Kamb, A; | A carcinoembryonic antigen-specific cell therapy selectively targets tumor cells with HLA loss of heterozygosity in vitro and in vivo | Science translational medicine | 35235342 | Informed consent for primary T cells and donor collection protocols were approved by an Institutional Review Board at AllCells or HemaCare. Peripheral blood mononuclear cells (PBMCs) were purified from Leukopaks purchased from AllCells or HemaCare, a | Full Paper |
2022 | Uehara, K;Harumoto, T;Makino, A;Koda, Y;Iwano, J;Suzuki, Y;Tanigawa, M;Iwai, H;Asano, K;Kurihara, K;Hamaguchi, A;Kodaira, H;Atsumi, T;Yamada, Y;Tomizuka, K; | Targeted delivery to macrophages and dendritic cells by chemically modified mannose ligand-conjugated siRNA | Nucleic acids research | 35524566 | are). Preparation of human monocyte-derived macrophages and DCs Two types of human monocyte-derived macrophages (h-Mo-Mφs) were generated as described in Supplementary Figure S1A. CD14+ monocytes (AllCells, Alameda, CA, USA) were cultured (37°C, 5% | Full Paper |
2022 | Pennycuick, A;Selway, H;Lam, J;Khan, K; | 331P Predicting outcomes following colorectal cancer resection: Using real-world data to empower adjuvant treatment decision making | Annals of Oncology | : Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. A. Vivancos: Financial Interests, Perso | Full Paper | |
2022 | Glaser, M;von Levetzow, C;Michels, S;Nogova, L;Katzenmeier, M;Wömpner, C;Schmitz, J;Bitter, E;Terjung, I;Passmann, E;Schaufler, D;Eisert, A;Fischer, R;Riedel, R;Weber, J;Hahne, S;Merkelbach-Bruse, S;Büttner, R;Wolf, J;Scheffler, M; | 1729P The biological effect of small-scale ROS1 aberrations: An in silico analysis | Annals of Oncology | MS. R. Riedel: Financial Interests, Personal, Funding: Boehringer Ingelheim, Loxo Oncology, Novartis, Lilly. J. Weber: Financial Interests, Personal, Advisory Board: Lilly. R. Büttner: Financial Interests, Personal, Ownership Interest: Targos Molecul | Full Paper | |
2022 | Poole, A;Karuppiah, V;Hartt, A;Haidar, JN;Moureau, S;Dobrzycki, T;Hayes, C;Rowley, C;Dias, J;Harper, S;Barnbrook, K;Hock, M;Coles, C;Yang, W;Aleksic, M;Lin, AB;Robinson, R;Dukes, JD;Liddy, N;Van der Kamp, M;Plowman, GD;Vuidepot, A;Cole, DK;Whale, AD;Chillakuri, C; | Therapeutic high affinity T cell receptor targeting a KRASG12D cancer neoantigen | Nature communications | 36088370 | ersberg, Germany). PBMCs were either purchased cryo-frozen from commercial suppliers (Cellular Technology Limited (CTL), OH & Tissue Solutions, Glasgow, UK) or prepared from healthy donor leukopaks (ALLCELLS, CA) by Ficoll density gradient centrifug | Full Paper |
2022 | Sano, Y;Azuma, Y;Tsunenari, T;Kayukawa, Y;Shinozuka, J;Fujii, E;Amano, J;Nishito, Y;Maruyama, T;Kinoshita, Y;Sakamoto, Y;Yoshida, A;Miyazaki, Y;Sato, Y;Teramoto-Seida, C;Ishiguro, T;Tanaka, T;Kitazawa, T;Endo, M; | Combination of T cell-redirecting bispecific antibody ERY974 and chemotherapy reciprocally enhances efficacy against non-inflamed tumours | Nature communications | 36071036 | (Tokyo, Japan). After an acclimatisation of 3 weeks, mice were irradiated (2.5 Gy; MBR-1520R-3; Hitachi Power Solutions Co. Ltd., Ibaraki, Japan) 1 day before transplantation of human CD34+ cells (AllCells, Alameda, CA, USA) via intravenous injection | Full Paper |
2022 | Badrani, JH;Strohm, AN;Lacasa, L;Civello, B;Cavagnero, K;Haung, YA;Amadeo, M;Naji, LH;Lund, SJ;Leng, A;Kim, H;Baum, RE;Khorram, N;Mondal, M;Seumois, G;Pilotte, J;Vanderklish, PW;McGee, HM;Doherty, TA; | RNA-binding protein RBM3 intrinsically suppresses lung innate lymphoid cell activation and inflammation partially through CysLT1R | Nature communications | 35908044 | &D Systems, Minneapolis, MN) in T cell media. Post-stimulation supernatant was collected and stored at −80 C for ELISA analysis. Human PBMCs were isolated from commercially purchased leukopacks (Allcells, Alameda, CA, USA). Human peripheral blood IL | Full Paper |
2022 | Li, F;Zhang, H;Wang, W;Yang, P;Huang, Y;Zhang, J;Yan, Y;Wang, Y;Ding, X;Liang, J;Qi, X;Li, M;Han, P;Zhang, X;Wang, X;Cao, J;Fu, YX;Yang, X; | T cell receptor β-chain-targeting chimeric antigen receptor T cells against T cell malignancies | Nature communications | 35882880 | ded by Dr. Justin Kline (University of Chicago). B16-OVA cells were kindly provided by Dr. Hans Schreiber (University of Chicago). Human peripheral blood mononuclear cells (PBMCs) were purchased from Allcells Biotechnology, Limited (Shanghai, China) | Full Paper |
2022 | Jo, S;Das, S;Williams, A;Chretien, AS;Pagliardini, T;Le Roy, A;Fernandez, JP;Le Clerre, D;Jahangiri, B;Chion-Sotinel, I;Rozlan, S;Dessez, E;Gouble, A;Dusséaux, M;Galetto, R;Duclert, A;Marcenaro, E;Devillier, R;Olive, D;Duchateau, P;Poirot, L;Valton, J; | Endowing universal CAR T-cell with immune-evasive properties using TALEN-gene editing | Nature communications | 35773273 | cell products and to allow their large-scale utilization against multiple malignancies for the benefit of a broader range of patients. Methods Materials Cryopreserved human PBMCs were acquired from ALLCELLS (cat# PB006F). PBMCs were cultured in CTS | Full Paper |
2022 | Cai, T;Gouble, A;Black, KL;Skwarska, A;Naqvi, AS;Taylor, D;Zhao, M;Yuan, Q;Sugita, M;Zhang, Q;Galetto, R;Filipe, S;Cavazos, A;Han, L;Kuruvilla, V;Ma, H;Weng, C;Liu, CG;Liu, X;Konoplev, S;Gu, J;Tang, G;Su, X;Al-Atrash, G;Ciurea, S;Neelapu, SS;Lane, AA;Kantarjian, H;Guzman, ML;Pemmaraju, N;Smith, J;Thomas-Tikhonenko, A;Konopleva, M; | Targeting CD123 in blastic plasmacytoid dendritic cell neoplasm using allogeneic anti-CD123 CAR T cells | Nature communications | 35484100 | The production of R&D grade batches of UCART123 was done using commercially available NHPBMC cells ordered from ALLCELLS or HEMACARE. These are normal human Save | Full Paper |
2022 | Salas, LA;Zhang, Z;Koestler, DC;Butler, RA;Hansen, HM;Molinaro, AM;Wiencke, JK;Kelsey, KT;Christensen, BC; | Enhanced cell deconvolution of peripheral blood using DNA methylation for high-resolution immune profiling | Nature communications | 35140201 | lymphocytes memory (CD4mem), T regulatory cells (Treg), T-cytotoxic lymphocytes naïve (CD8nv), T-cytotoxic lymphocytes memory (CD8mem), and natural killer lymphocytes (NK) cells] were purchased from AllCells corporation (Alameda, CA, USA), StemExpre | Full Paper |
2022 | Wiesweg, M;Hense, J;Darwiche, K;Michels, S;Hautzel, H;Kobe, C;Metzenmacher, M;Herold, T;Zaun, G;Laue, K;Drzezga, A;Schildhaus, H;Wolf, J;Herrmann, K;Schuler, M; | 1171P A phase II theranostic study with osimertinib in patients with EGFR-mutated non-small cell lung cancer (NSCLC) progressing on EGFR tyrosine kinase inhibitors (TKI) and undetectable EGFR T790M (THEROS) | Annals of Oncology | ovartis Oncology; Financial Interests, Personal, Full or part-time Employment: Targos Molecular Pathology, Inc. J. Wolf: Financial Interests, Personal, Advisory Board: Amgen, AstraZeneca, Bayer, Blueprint, BMS, Boehringer Ingelheim, Chugai, Daiichi S | Full Paper | |
2022 | Chansaenroj, A;Adine, C;Charoenlappanit, S;Roytrakul, S;Sariya, L;Osathanon, T;Rungarunlert, S;Urkasemsin, G;Chaisuparat, R;Yodmuang, S;Souza, GR;Ferreira, JN; | Magnetic bioassembly platforms towards the generation of extracellular vesicles from human salivary gland functional organoids for epithelial repair | Bioactive materials | 35387159 | d EV; and (3) to identify novel signaling cues in EV that may optimize organoid development towards epithelial SG repair. 2 Materials and methods 2.1 Adult stem cell culture hDPSC were obtained from AllCells (Alameda, CA, USA) and cultured in Dulbec | Full Paper |
2022 | Kavanagh, ME;Horning, BD;Khattri, R;Roy, N;Lu, JP;Whitby, LR;Ye, E;Brannon, JC;Parker, A;Chick, JM;Eissler, CL;Wong, AJ;Rodriguez, JL;Rodiles, S;Masuda, K;Teijaro, JR;Simon, GM;Patricelli, MP;Cravatt, BF; | Selective inhibitors of JAK1 targeting an isoform-restricted allosteric cysteine | Nature chemical biology | 36097295 | In vitro TE50 values for JAK1_C817 and TYK2_C838 were obtained by treating 500 µg cell lysate generated from primary human PBMCs (AllCells), Jurkat T cells (ATCC, #TIB-152), or MDA-MB-468 breast cancer cells (ATCC, #HTB-132), with DMSO or compound fo | Full Paper |
2022 | Lam, J;Lee, B;Yu, J;Kwee, BJ;Kim, Y;Kim, J;Choi, Y;Yoon, JS;Kim, Y;Baek, K;Jeon, NL;Sung, KE; | A microphysiological system-based potency bioassay for the functional quality assessment of mesenchymal stromal cells targeting vasculogenesis | Biomaterials | 36201944 | Human bone marrow-derived MSCs were obtained from eight different donors purchased from RoosterBio (RB9, RB12, RB14, RB16; Frederick, MD) at passage 1 (P1), Lonza (8F560, 167,696; Walkersville, MD) at passage 2 (P2), and AllCells (PCBM1632, PCBM1641; | Full Paper |
2022 | Han, C;Khodadadi-Jamayran, A;Lorch, AH;Jin, Q;Serafin, V;Zhu, P;Politanska, Y;Sun, L;Gutierrez-Diaz, BT;Pryzhkova, MV;Abdala-Valencia, H;Bartom, ET;Buldini, B;Basso, G;Velu, SE;Sarma, K;Mattamana, BB;Cho, BK;Obeng, RC;Goo, YA;Jordan, PW;Tsirigos, A;Zhou, Y;Ntziachristos, P; | SF3B1 homeostasis is critical for survival and therapeutic response in T cell leukemia | Science advances | 35061527 | ing PCR to detect the TCRb-NOTCH1 translocation (TCRBJ2S4CUTLL1F: 5′-GGACCCGGCTCTCAGTGCT-3′, NOTCH1CUTTL1R: 5′-TCCCGCCCTCCAAAATAAGG-3′). Human CD3+, CD8+, and CD4+ T cells were purchased from AllCells.com (Alameda, CA) or from Astarte Biologics (Both | Full Paper |
2022 | Pan, Z;Chen, J;Xiao, X;Xie, Y;Jiang, H;Zhang, B;Lu, H;Yuan, Y;Han, L;Zhou, Y;Zong, H;Wang, L;Sun, R;Zhu, J; | Characterization of a novel bispecific antibody targeting tissue factor-positive tumors with T cell engagement | Acta pharmaceutica Sinica. B | 35847491 | for the T cell activation test were bought from AllCells (Shanghai, China). PBMC used in other experiments were isolated from leukocyte concentrate (AllCells) using Ficoll-Paque PLUS ( Save;nded conditions and used within 2 months after resuscitatio | Full Paper |
2022 | Hu, J;Zhong, Y;Shang, X; | A versatile and scalable single-cell data integration algorithm based on domain-adversarial and variational approximation | Briefings in bioinformatics | 34585247 | Integration of two modal of multimodal data on human PBMCs of a healthy female donor aged 25 that were obtained by 10x Genomics from AllCells. UMAP visualization on integrated Save | Full Paper |
2022 | Peng, S;Hu, P;Xiao, YT;Lu, W;Guo, D;Hu, S;Xie, J;Wang, M;Yu, W;Yang, J;Chen, H;Zhang, X;Zhu, Y;Wang, Y;Yang, Y;Zhu, G;Chen, S;Wang, J;Zhang, B;Chen, W;Wu, H;Sun, Z;Ding, T;Zhang, H;Yi, Z;Liu, M;Ren, S; | Single-Cell Analysis Reveals EP4 as a Target for Restoring T-Cell Infiltration and Sensitizing Prostate Cancer to Immunotherapy | Clinical cancer research : an official journal of the American Association for Cancer Research | 34740924 | Purpose: Immunotherapies targeting immune checkpoint molecules have shown promising treatment for a subset of cancers; however, many "cold" tumors, such as prostate cancer, Save | Full Paper |
2022 | Siegel, RJ;Singh, AK;Panipinto, PM;Shaikh, FS;Vinh, J;Han, SU;Kenney, HM;Schwarz, EM;Crowson, CS;Khuder, SA;Khuder, BS;Fox, DA;Ahmed, S; | Extracellular sulfatase-2 is overexpressed in rheumatoid arthritis and mediates the TNF-?-induced inflammatory activation of synovial fibroblasts | Cellular & molecular immunology | 36068294 | Human serum samples were purchased from commercial vendors (Discovery Life Sciences, Newtown, PA; Innovative Research, Novi, MI; or Sanguine Biosciences, Los Angeles, CA) or generously shared by Dr. Cynthia Crowson (Mayo Clinic, Rochester, MN) | Full Paper |
2022 | Gyurdieva, A;Zajic, S;Chang, YF;Houseman, EA;Zhong, S;Kim, J;Nathenson, M;Faitg, T;Woessner, M;Turner, DC;Hasan, AN;Glod, J;Kaplan, RN;D'Angelo, SP;Araujo, DM;Chow, WA;Druta, M;Demetri, GD;Van Tine, BA;Grupp, SA;Fine, GD;Eleftheriadou, I; | Biomarker correlates with response to NY-ESO-1 TCR T cells in patients with synovial sarcoma | Nature communications | 36075914 | s were digitally obtained using CaseViewer (3D Histech, Budapest, Hungary). Protein expression by IHC NY-ESO-1 staining was performed using the E978 clone (Sigma, Catalog #: N2038, at 1??g/mL) at QualTek Laboratory (Goleta, CA; now part of Discovery | Full Paper |
2022 | Ni, Z;Sun, P;Zheng, J;Wu, M;Yang, C;Cheng, M;Yin, M;Cui, C;Wang, G;Yuan, L;Gao, Q;Li, Y; | JNK Signaling Promotes Bladder Cancer Immune Escape by Regulating METTL3-Mediated m6A Modification of PD-L1 mRNA | Cancer research | 35502544 | CD8+ T cells (catalog no. PB009-3F-C) were obtained from AllCells (Shanghai) and cultured in ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies, catalog no. 10981). Seventy-two hours after activation with ImmunoCult Human CD3/CD28/CD2 T Cel | Full Paper |
2022 | Ge, X;Yost, SE;Lee, JS;Frankel, PH;Ruel, C;Cui, Y;Murga, M;Tang, A;Martinez, N;Chung, S;Yeon, C;Stewart, D;Li, D;Rajurkar, S;Somlo, G;Mortimer, J;Waisman, J;Yuan, Y; | Phase II Study Combining Pembrolizumab with Aromatase Inhibitor in Patients with Metastatic Hormone Receptor Positive Breast Cancer | Cancers | 36077811 | mal TILs were measured by hematoxylin and eosin (H&E) staining of formalin-fixed paraffin-embedded (FFPE) tumor biopsies according to the International TIL Work Group guideline [16]. PD-L1 analysis: QualTek Molecular Laboratory (Goleta, CA, USA) per | Full Paper |
2022 | Shirk, B;Shirk, P;Furlong, R;Scully, E;Wu, K;Siegfried, B; | Gene Editing of the ABC Transporter/White Locus Using Crispr/Cas9-Mediated Mutagenesis in the Indian Meal Moth | SSRN Electronic Journal | y and sequencing, and Kris Hartzer for laboratory assistance. Library 497 preparation and sequencing were performed at HudsonAlpha Genomic Services Lab 498 (Huntsville, AL). We also thank Karen Garren for her work to isolate the P. interpunctella 499 | Full Paper | |
2022 | Kanter, J;Thompson, AA;Pierciey, FJ;Hsieh, M;Uchida, N;Leboulch, P;Schmidt, M;Bonner, M;Guo, R;Miller, A;Ribeil, JA;Davidson, D;Asmal, M;Walters, MC;Tisdale, JF; | Lovo-cel gene therapy for sickle cell disease: Treatment process evolution and outcomes in the initial groups of the HGB-206 study | American journal of hematology | 36161320 | MCW served as medical director for AllCells and as a consultant for AllCells, Ensoma, Vertex, and BioLabs. The remaining authors declare no competing financial interests. Save | Full Paper |
2022 | Wei, Y;Kanagal-Shamanna, R;Zheng, H;Bao, N;Lockyer, PP;Class, CA;Darbaniyan, F;Lu, Y;Lin, K;Yang, H;Montalban-Bravo, G;Ganan-Gomez, I;Soltysiak, KA;Do, KA;Colla, S;Garcia-Manero, G; | Cooperation between KDM6B overexpression and TET2 deficiency in the pathogenesis of chronic myelomonocytic leukemia | Leukemia | 35697791 | Materials and methods Human samples BM specimens were obtained from the MD Anderson Cancer Center tissue bank following institutional guidelines. BM samples from healthy individuals were obtained from AllCells (Emeryville, CA) | Full Paper |
2022 | Lu, M;Xia, L;Elmansy, N;Clementelli, C;Tremblay, D;Hoffman, R; | Combined Drug Targeting of p53-dependent and -independent Pathways Depletes Myelofibrosis Hematopoietic Stem/Progenitor Cells | Leukemia | 34642468 | met the World Health Organization criteria for the diagnosis of MF [41, 42]. Patient characteristics are provided in Supplemental Table 1. Normal donor (ND) human bone marrow (BM) was purchased from AllCells (Emeryville, CA). The experiments in which | Full Paper |
2022 | Bae, J;Accardi, F;Hideshima, T;Tai, YT;Prabhala, R;Shambley, A;Wen, K;Rowell, S;Richardson, PG;Munshi, NC;Anderson, KC; | Targeting LAG3/GAL-3 to overcome immunosuppression and enhance anti-tumor immune responses in multiple myeloma | Leukemia | 34290359 | roval by the Institutional Review Board at Dana-Farber Cancer Institute (Boston, MA). In addition, healthy individuals BM [N = 5] or leukapheresis [N = 12] products were purchased from either AllCells (Alameda, CA) or the Blood Donor Center at Boston | Full Paper |
2022 | Huang, G;Cheng, Z;Hildebrand, A;Wang, C;Cimini, M;Roy, R;Lucchese, AM;Benedict, C;Mallaredy, V;Magadum, A;Joladarashi, D;Thej, C;Gonzalez, C;Trungcao, M;Garikipati, VNS;Elrod, JW;Koch, WJ;Kishore, R; | Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition | Theranostics | 35673580 | days, db/+ EPCs were cultured at 37°C, 5% CO2 atmosphere. Male mice were used to avoid the reproductive hormone effect in female mice. Human CD34+ Hematopoietic stem cells (HSC) were purchased from AllCells, LLC and were cultured in a human hematopoi | Full Paper |
2022 | Shen, Y;Eng, JS;Fajardo, F;Liang, L;Li, C;Collins, P;Tedesco, D;Nolan-Stevaux, O; | Cancer cell-intrinsic resistance to BiTE therapy is mediated by loss of CD58 costimulation and modulation of the extrinsic apoptotic pathway | Journal for immunotherapy of cancer | 35296559 | Target cells were cocultured with freshly thawed human CD3 + T cells (ALLCELLS) and increasing concentrations of TCEs. Assay conditions were effector-to-target ratio (E:T)=2:1 and Save | Full Paper |
2022 | Froning, KJ;Sereno, A;Huang, F;Demarest, SJ; | Generalizable design parameters for soluble T cell receptor-based T cell engagers | Journal for immunotherapy of cancer | 35260435 | ously11 with minor modifications throughout the protocol. Saos-2 and HCT-116 cells were cultured as described earlier. Primary naïve T cells were from StemExpress (cat#PB03020C, Donor#D001003581) or AllCells (cat# PB009-1F, Lt# 3009286). For the assa | Full Paper |
2022 | Wu, ZH;Li, N;Mei, XF;Chen, J;Wang, XZ;Guo, TT;Chen, G;Nie, L;Chen, Y;Jiang, MZ;Wang, JT;Wang, HB; | Preclinical characterization of the novel anti-SIRPα antibody BR105 that targets the myeloid immune checkpoint | Journal for immunotherapy of cancer | 35256517 | Allogeneic PBMCs (AllCells) from different donors were added at a dendritic cell:PBMCs ratio of 1:20. Indicated mAbs were added at a concentration of 10 µg/mL immediately, and the Save | Full Paper |
2022 | Lee, KM;Lin, CC;Servetto, A;Bae, J;Kandagatla, V;Ye, D;Kim, G;Sudhan, DR;Mendiratta, S;González Ericsson, PI;Balko, JM;Lee, J;Barnes, S;Malladi, VS;Tabrizi, S;Reddy, SM;Yum, S;Chang, CW;Hutchinson, KE;Yost, SE;Yuan, Y;Chen, ZJ;Fu, YX;Hanker, AB;Arteaga, CL; | Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer | Cancer immunology research | 35561311 | After a week, 1 A 107 human PBMCs (hPBMC; ALLCELLS) were injected intravenously. Two weeks later, SUM159PT tumors were harvested for analysis of tumor-infiltrating immune Save | Full Paper |
2022 | Li, W;Yang, S;Xu, P;Zhang, D;Tong, Y;Chen, L;Jia, B;Li, A;Lian, C;Ru, D;Zhang, B;Liu, M;Chen, C;Fu, W;Yuan, S;Gu, C;Wang, L;Li, W;Liang, Y;Yang, Z;Ren, X;Wang, S;Zhang, X;Song, Y;Xie, Y;Lu, H;Xu, J;Wang, H;Yu, W; | SARS-CoV-2 RNA elements share human sequence identity and upregulate hyaluronan via NamiRNA-enhancer network | EBioMedicine | 35124429 | Human umbilical vein endothelial cells (HUVEC) were cultured in the commercial culture medium (AllCells, Cat# H-004). VERO C1008 (E6) (Cat# GNO17) and its culture medium MEM Save;ed with 10% FBS (Gibco, Cat# 10270-106) and 1% Penicillin-Streptomyci | Full Paper |
2022 | Zhang, C;Chen, Z;Liu, J;Wu, M;Yang, J;Zhu, Y;Lu, W;Ruan, C; | 3D-printed pre-tapped-hole scaffolds facilitate one-step surgery of predictable alveolar bone augmentation and simultaneous dental implantation | Composites Part B: Engineering | The human bone marrow mesenchymal stem cells (hBMSCs, obtained from Allcells, Silicon Valley, CA) were cultured in α-MEM (Gibco, USA) supplemented with 1% penicillin-streptomycin (Gibco, USA) and 10% fetal bovine serum (FBS) (Gibco, USA) in an atmosp | Full Paper | |
2022 | Petaroudi, M;Rodrigo-Navarro, A;Dobre, O;Dalby, MJ;Salmeron-Sanchez, M; | Living Biomaterials to Engineer Hematopoietic Stem Cell Niches | Advanced healthcare materials | 35933595 | Fresh, cryopreserved bone marrow CD34+ cells isolated from the posterior iliac crest of healthy and consenting donors were purchased from AllCells. All cultures containing CD34+ Save | Full Paper |
2022 | Suyama, T;Takemoto, Y;Miyauchi, H;Kato, Y;Matsuzaki, Y;Kato, R; | Morphology-based noninvasive early prediction of serial-passage potency enhances the selection of clone-derived high-potency cell bank from mesenchymal stem cells | Inflammation and regeneration | 36182958 | Bone marrow mononuclear cells were prepared from bone marrow aspirate (AllCells, Alameda, USA) collected from healthy donors using density gradient centrifugation with Ficoll (GE Save Cached | Full Paper |
2022 | Wang, J;Jiang, J;Yang, X;Zhou, G;Wang, L;Xiao, B; | Tethering Piezo channels to the actin cytoskeleton for mechanogating via the cadherin-β-catenin mechanotransduction complex | Cell reports | 35139384 | Experimental models: Cell lines HEK293T Xiao et al., 2011 N/A MDCK Xu Tan Lab in Tsinghua University N/A HUVEC Purchased from Allcells N/A | Full Paper |
2022 | Sun, Y;Wang, T;Lv, Y;Li, J;Jiang, X;Jiang, J;Zhang, D;Bian, W;Zhang, C; | MALAT1 promotes platelet activity and thrombus formation through PI3k/Akt/GSK-3β signalling pathway | Stroke and vascular neurology | 36241224 | Allcells Biotechnology (Shanghai, China) was the supplier of the Cord Blood-Stem/Progenitor (CD34+) cells that were used in this study. Serum-free expansion medium (StemCell Technologies, Canada) was used in the cultivation of CD34+ cells. To facilit | Full Paper |
2022 | Manshouri, T;Veletic, I;Li, P;Yin, CC;Post, SM;Verstovsek, S;Estrov, Z; | GLI1 activates pro-fibrotic pathways in myelofibrosis fibrocytes | Cell death & disease | 35595725 | ld Health Organization revised criteria [1]. Control BM biopsies were obtained from morphologically normal BM donors and control BM aspirates were obtained from commercially available healthy donors (AllCells, Alameda, CA, USA). The patients’ and con | Full Paper |
2022 | Zhang, J;Liu, F;Yang, Y;Yu, N;Weng, X;Yang, Y;Gong, Z;Huang, S;Gan, L;Sun, S;Zhang, X;Gong, Y;Liu, Y;Guo, W; | Integrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancer | Cell death & disease | 35449126 | ze the results using the positive control contained in the different membranes. T-cell amplification and tumor-reactive T cell killing assay Peripheral blood mononuclear cells (PBMCs) purchased from AllCells (China) were activated with anti-CD3 anti | Full Paper |
2022 | Liu, P;Zhang, Q;Mi, J;Wang, S;Xu, Q;Zhuang, D;Chen, W;Liu, C;Zhang, L;Guo, J;Wu, X; | Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246 | Stem cell research & therapy | 35241153 | Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Cat. H-001F, AllCells) and were cultured in DMEM (Cat. SH30021.01, Hyclone) with 4.5 g/L D-Glucose Save | Full Paper |
2022 | McLemore, AF;Hou, HA;Meyer, BS;Lam, NB;Ward, GA;Aldrich, AL;Rodrigues, MA;Vedder, A;Zhang, L;Padron, E;Vincelette, ND;Sallman, DA;Abdel-Wahab, O;List, AF;McGraw, KL; | Somatic gene mutations expose cytoplasmic DNA to co-opt the cGAS/STING/NLRP3 axis in myelodysplastic syndromes | JCI insight | 35788117 | using Ficoll-Paque (GE Healthcare) from patients with MDS and healthy donors who provided informed consent at the Moffitt Cancer Center or NTUH or were purchased from AllCells. Save Cached | Full Paper |
2022 | Xie, J;Gui, X;Deng, M;Chen, H;Chen, Y;Liu, X;Ku, Z;Tan, L;Huang, R;He, Y;Zhang, B;Lewis, C;Chen, K;Xu, L;Xu, J;Huang, T;Liao, XC;Zhang, N;An, Z;Zhang, CC; | Blocking LAIR1 signaling in immune cells inhibits tumor development | Frontiers in immunology | 36211388 | Human allogeneic pan T cells (PB009-1F, Allcells) were stained with Carboxy fluorescein succinimidyl ester (CFSE) (C34554, Thermo Fisher Scientific), and mixed with 3 x10 5 mature Save | Full Paper |
2022 | Hu, JF;Wang, ZW;Liao, CY;Chen, ZW;Kang, FP;Lin, CF;Lin, TS;Huang, L;Tian, YF;Chen, S; | Induced expression of CCL19 promotes the anti-tumor ability of CAR-T cells by increasing their infiltration ability | Frontiers in immunology | 35990619 | (CRL-1682, ATCC) cells were obtained from ATCC and cultured in RPMI-1640 medium (cat. no. 11875093; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. Human T cells were obtained from Allcells (cat. no. PB009-CD3-F). Both of the cells | Full Paper |
2022 | Bjornson-Hooper, ZB;Fragiadakis, GK;Spitzer, MH;Chen, H;Madhireddy, D;Hu, K;Lundsten, K;McIlwain, DR;Nolan, GP; | A Comprehensive Atlas of Immunological Differences Between Humans, Mice, and Non-Human Primates | Frontiers in immunology | 35359965 | apeutics between humans and model organisms will greatly improve the evaluation of therapeutic candidates. Materials and Methods Blood Venous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human sub | Full Paper |
2022 | Peng, H;Nerreter, T;Mestermann, K;Wachter, J;Chang, J;Hudecek, M;Rader, C; | ROR1-targeting switchable CAR-T cells for cancer therapy | Oncogene | 35859167 | Pan-T cells were isolated from fresh PBMC of healthy donors (Allcells) by negative selection (Pan T Cell Isolation Kit; Miltenyi Biotec), activated using Dynabeads Human T-Activator Save | Full Paper |
2022 | Zhang, M;Zhu, Y;Zhu, J;Xie, Y;Wu, R;Zhong, J;Qiu, Z;Jiang, L; | circ_0086296 induced atherosclerotic lesions via the IFIT1/STAT1 feedback loop by sponging miR-576-3p | Cellular & molecular biology letters | 36138395 | HUVECs were obtained from AllCells (Shanghai, China) as previously reported [7, 33]. HUVECs were seeded in EC complete growth medium at 37 C. Then, 100 μg/mL ox-LDL was Save Cached | Full Paper |
2022 | Abdul-Aziz, A;Weigel, C;Kovacs, A;Wu, Y;Byrd, J;Hertlein, E;Oakes, C; | P381: DNA METHYLATION PROFILING OF MESENCHYMAL STROMAL CELLS ISOLATED FROM FEMURAL HEAD BONE MARROW VERSUS BONE MARROW ASPIRATES: RELEVANCE FOR AML STUDY BASED CONTROLS | HemaSphere | Aspirate-derived BMSCs (n= 7) were expanded from bone marrow mononuclear cells obtained from a commercial source (AllCells). For genome-wide DNA methylation analyses, the Save | Full Paper | |
2022 | Owens, TD;Brameld, KA;Verner, EJ;Ton, T;Li, X;Zhu, J;Masjedizadeh, MR;Bradshaw, JM;Hill, RJ;Tam, D;Bisconte, A;Kim, EO;Francesco, M;Xing, Y;Shu, J;Karr, D;LaStant, J;Finkle, D;Loewenstein, N;Haberstock-Debic, H;Taylor, MJ;Nunn, P;Langrish, CL;Goldstein, DM; | Discovery of Reversible Covalent Bruton's Tyrosine Kinase Inhibitors PRN473 and PRN1008 (Rilzabrutinib) | Journal of medicinal chemistry | 35302767 | The ability of selected compounds to block BCR-driven activation of B cells was assessed by expression of the CD69 surface marker, as previously described. (37) Briefly, HWB (AllCells, Alameda, CA, USA) pretreated for 1 h with serial dilutions of tes | Full Paper |
2022 | Zheng, L;Wang, H;Zuo, P;Liu, Y;Xu, H;Ye, BC; | Rapid On-Chip Isolation of Cancer-Associated Exosomes and Combined Analysis of Exosomes and Exosomal Proteins | Analytical chemistry | 35575685 | The biotin-labeled rabbit antihuman CD63 antibody (anti-CD63) was purchased from AllCells Biotech Co., Ltd. (Shanghai, China). The horseradish peroxidase (HRP)-conjugated secondary antibody, rabbit anti-human CD9 polyclonal antibody, and bicinchonini | Full Paper |
2022 | Avanzino, BC;Prabhakar, K;Dalvi, P;Hartstein, S;Kehm, H;Balasubramani, A;Boudreau, AA;Buelow, B;Chang, K;Davison, LM;Iyer, S;Kalwit, V;Lewis Wilson, K;Malik-Chaudhry, HK;Pierson, W;Pineda, G;Rangaswamy, US;Saiganesh, S;Schellenberger, U;Ugamraj, HS;Yabut, RD;Buelow, R;Chapman, J;Trinklein, ND;Harris, KE; | A T-cell engaging bispecific antibody with a tumor-selective bivalent folate receptor alpha binding arm for the treatment of ovarian cancer | Oncoimmunology | 36016696 | In a similar follow-on study, NCG mice were injected with activated human PBMCs (AllCells). Prior to injection, PBMCs were activated for 3 d with 1 µg/mL plate-coated anti-CD3 ( Save | Full Paper |
2022 | Xue, W;Zhang, Q;Chen, Y;Zhu, Y; | Hydrogen Sulfide Improves Angiogenesis by Regulating the Transcription of pri-miR-126 in Diabetic Endothelial Cells | Cells | 36078059 | The HUVECs from passages 4 to 7 were used in the experiment and were cultured in endothelial completed medium (ALLCELLS, Shanghai, China) at 37 C in 5% CO 2 . The HUVECs Save Cached | Full Paper |
2022 | Sharma, M;Bellio, MA;Benny, M;Kulandavelu, S;Chen, P;Janjindamai, C;Han, C;Chang, L;Sterling, S;Williams, K;Damianos, A;Batlahally, S;Kelly, K;Aguilar-Caballero, D;Zambrano, R;Chen, S;Huang, J;Wu, S;Hare, JM;Schmidt, A;Khan, A;Young, K; | Mesenchymal Stem Cell-derived Extracellular Vesicles Prevent Experimental Bronchopulmonary Dysplasia Complicated By Pulmonary Hypertension | Stem cells translational medicine | 35758326 | es, and isotype control was performed on P2 WJ-MSC to confirm the MSC identity. BM-MSC Production BM-MSCs were isolated from fresh adult bone marrow aspirates, and obtained from a commercial source (AllCells, Alameda, CA). Briefly, bone marrow was p | Full Paper |
2022 | Ohori-Morita, Y;Niibe, K;Limraksasin, P;Nattasit, P;Miao, X;Yamada, M;Mabuchi, Y;Matsuzaki, Y;Egusa, H; | Novel Mesenchymal Stem Cell Spheroids with Enhanced Stem Cell Characteristics and Bone Regeneration Ability | Stem cells translational medicine | 35267026 | heir therapeutic potential in bone regeneration. Materials and Methods BM-MSC Adherent Culture Human BM-MSCs of an 18-year-old male (donor 1; Lonza, Basel, Switzerland), a 22-year-old male (donor 2; AllCells, Alameda, CA, USA), and a 24-year-old mal | Full Paper |
2022 | Stratmann, S;Yones, SA;Garbulowski, M;Sun, J;Skaftason, A;Mayrhofer, M;Norgren, N;Herlin, MK;Sundström, C;Eriksson, A;Höglund, M;Palle, J;Abrahamsson, J;Jahnukainen, K;Munthe-Kaas, MC;Zeller, B;Tamm, KP;Cavelier, L;Komorowski, J;Holmfeldt, L; | Transcriptomic analysis reveals proinflammatory signatures associated with acute myeloid leukemia progression | Blood advances | 34619772 | children (Table 1). Detailed biological characteristics and supporting clinical information are reported in supplemental Tables 2 and 3. CD34-expressing bone marrow (BM) cells from 5 healthy donors (AllCells Inc., Alameda, CA) were used as normal con | Full Paper |
2022 | Jeon, E;Choi, D;Choi, S;Won, J;Jo, Y;Kim, H;Jung, Y;Shin, S;Min, H;Choi, H;Lee, M;Park, Y;Chung, J;Jin, H; | Enhancing adoptive T‐cell therapy with fucoidan‐based IL ‐2 delivery microcapsules | Bioengineering & Translational Medicine | Cryopreserved human peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryvill, CA). Total CD3+T cells were isolated by negative selection with a magnetic isolation kit and CD4+T, CD8+T, and regulatory T (Treg) cells were then | Full Paper | |
2022 | Lun, Y;Zhou, Y;Li, Q;Chen, P;Huang, Y;Ye, G; | Preparation and characterization of a magnetic microsphere synthesized from sucrose allyl ether for transcatheter arterial embolization | Materials Today Chemistry | Human umbilical vein endothelial cells (HUVECs, AllCells, US) were subcultured in a low glucose Dulbecco's modified eagle medium containing 10% fetal bovine serum and 1% penicillin/streptomycin (100 U·mL−1) in an atmosphere of 310.15 K and 5% CO2 | Full Paper | |
2022 | Lu, W;Zhou, C;Ma, Y;Li, J;Jiang, J;Chen, Y;Dong, L;He, F; | Improved osseointegration of strontium-modified titanium implants by regulating angiogenesis and macrophage polarization | Biomaterials science | 35384947 | HUVECs together with complete and basal medium were purchased from Allcells Bio (Shanghai, China). The murine macrophage cell lines (Raw264.7) were obtained from the Save | Full Paper |
2022 | Zhou, Y;Li, XH;Xue, WL;Jin, S;Li, MY;Zhang, CC;Yu, B;Zhu, L;Liang, K;Chen, Y;Tao, BB;Zhu, YZ;Wang, MJ;Zhu, YC; | YB-1 Recruits Drosha to Promote Splicing of pri-miR-192 to Mediate the Proangiogenic Effects of H2S | Antioxidants & redox signaling | 35044231 | HUVECs were purchased from Allcells (Shanghai, China) and cultured in endothelial basal medium (EBM; Allcells, Shanghai, China) supplemented with hydrocortisone, bovine brain Save | Full Paper |
2022 | Li, J;Hong, T;Wei, Y;Guo, L;Lee, M;Yang, H;Class, C;Yang, Y;Wang, X;He, H;Siwko, S;You, MJ;Zhou, Y;Garcia-Manero, G;Huang, Y; | Aberrant DNA hydroxymethylation reshapes transcription factor binding in myeloid neoplasms | Clinical epigenetics | 35765052 | BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). BM mononuclear cells were isolated using a Ficoll-Paque density gradient media (GE, Sweden). Save Cached | Full Paper |
2022 | Fang, J;Li, R;Zhang, Y;Oduro, PK;Li, S;Leng, L;Wang, Z;Rao, Y;Niu, L;Wu, HH;Wang, Q; | Aristolone in Nardostachys jatamansi DC. induces mesenteric vasodilation and ameliorates hypertension via activation of the KATP channel and PDK1-Akt-eNOS pathway | Phytomedicine : international journal of phytotherapy and phytopharmacology | 35738117 | Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells Biotech Co. Ltd. (Shanghai, China) and cultured in Endothelial Cell Medium (ECM; AllCells Biotech Co. Ltd., Shanghai, China) with 10% fetal bovine serum, 1% endothelial growt | Full Paper |
2022 | Liang, W;Chen, J;Zheng, H;Lin, A;Li, J;Wu, W;Jie, Q; | MiR-199a-5p-containing macrophage-derived extracellular vesicles inhibit SMARCA4 and alleviate atherosclerosis by reducing endothelial cell pyroptosis | Cell biology and toxicology | 35930100 | Human monocyte-derived macrophages (hMDMs, PB-MDM-001F, AllCells) were purchased from Milestone Biotechnologies (Shanghai, China). The hMDMs were cultured at 37 °C in RPMI1640 medium (supplemented with 10% FBS and 1% penicillin/streptomycin) with 5% | Full Paper |
2022 | Wang, J;Li, C;He, K;Kuang, Z;Lu, J;Yao, Y;He, F;Li, N;Li, L;Fu, F;Wu, Z;Zhou, S;Kang, D;Qiu, X;Wu, M;Liu, Y;Cao, X;Xu, M;Chen, B;Wu, W;Guo, F; | Characterization of anti-CD79b/CD3 bispecific antibody, a potential therapy for B cell malignancies | Cancer immunology, immunotherapy : CII | 35963895 | Materials and methods Reagents IBI38D9 and human IgG1 were produced by Innovent Biologics Co., Ltd. (Suzhou, China). Human IgG was purchased from Equitech-Bio. Peripheral blood mononuclear cells (PBMCs) were purchased from the AllCells | Full Paper |
2022 | Ni, H;Cao, L;Wu, Z;Wang, L;Zhou, S;Guo, X;Gao, Y;Jing, H;Wu, M;Liu, Y;Ding, J;Zhang, P;Zhou, Y;Chen, B;Xiong, Y;Sun, J;Prinz, B;Baruah, H;Geoghegan, J;Yu, M;Wu, W;Liu, J; | Combined strategies for effective cancer immunotherapy with a novel anti-CD47 monoclonal antibody | Cancer immunology, immunotherapy : CII | 34165607 | CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMCs, Allcells) and then differentiated into macrophages following incubation for 7 days with granulocyte- Save | Full Paper |
2022 | Wu, ZH;Li, N;Gao, ZZ;Chen, G;Nie, L;Zhou, YQ;Jiang, MZ;Chen, Y;Chen, J;Mei, XF;Hu, F;Wang, HB; | Development of the Novel Bifunctional Fusion Protein BR102 That Simultaneously Targets PD-L1 and TGF-β for Anticancer Immunotherapy | Cancers | 36230887 | IgG1 isotype control was expressed by Biointron Biological (Taizhou, China). 2.2. Cell Lines and Cell Culture Dendritic cells (DCs) and peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Shanghai, China). TF-1 cells were from t | Full Paper |
2022 | Wang, X;Chen, G;Nie, L;Wu, Z;Wang, X;Pan, C;Chen, X;Zhao, X;Zhu, J;He, Q;Wang, H; | IL-2K35C-moFA, a Long-Acting Engineered Cytokine with Decreased Interleukin 2 Receptor α Binding, Improved the Cellular Selectivity Profile and Antitumor Efficacy in a Mouse Tumor Model | Cancers | 36230665 | Next, 100 μL CellTiter-Glo (G7572, Promega Corporation, Madison, WI, USA) was added; luminescence was recorded suing a Spectramax M5. 2.8. p-STAT5 Assay in Treg and CD8+ T Cell Tregs (PB009-4F-2, Allcells, Shanghai, China) at a density of 1 × 106 c | Full Paper |
2022 | Sekhri, P;Ledezma, DK;Shukla, A;Sweeney, EE;Fernandes, R; | The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma Cells | Cancers | 35326601 | Healthy donor peripheral blood leukopaks were purchased from AllCells (Alameda, CA, USA) along with the HLA report. Peripheral blood mononuclear cells (PBMCs) were isolated by Save | Full Paper |
2022 | Ning, K;Roy, A;Cheng, F;Xu, P;Kleiboeker, S;Escalante, CR;Wang, J;Qiu, J; | High-Throughput Screening Identifies Inhibitors for Parvovirus B19 Infection of Human Erythroid Progenitors | Journal of virology | 34669461 | Primary human CD34+ hematopoietic stem cells (HSCs), which were isolated from the bone marrow of a healthy human donor, were purchased from AllCells LLC (Alameda, CA). B19V-containing plasma samples were obtained from ViraCor Eurofins Laboratories (L | Full Paper |
2022 | Liu, Y;Tsang, K;Mays, M;Hansen, G;Chiecko, J;Crames, M;Wei, Y;Zhou, W;Fredrick, C;Hu, J;Liu, D;Gebhard, D;Huang, ZF;Datar, A;Kronkaitis, A;Gueneva-Boucheva, K;Seeliger, D;Han, F;Sen, S;Kasturirangan, S;Scheer, JM;Nixon, AE;Panavas, T;Marlow, MS;Kumar, S; | An adapted consensus protein design strategy for identifying globally optimal biotherapeutics | mAbs | 35613320 | isolated from human peripheral blood mononuclear cells (PBMCs) using a negative selection kit (EasySep). PBMCs were isolated from Leukopak collected from healthy volunteers, which were obtained from ALLcells. Redirected T-cell cytotoxicity was assaye | Full Paper |
2022 | Zhao, J;Jiang, L;Yang, H;Deng, L;Meng, X;Ding, J;Yang, S;Zhao, L;Xu, W;Wang, X;Zhu, Z;Huang, H; | A strategy for the efficient construction of anti-PD1-based bispecific antibodies with desired IgG-like properties | mAbs | 35239451 | #F9512) at 4°C for 1 h. Cells were washed three times and then analyzed on the CytoFLEX Cytometer System (Beckman Coulter). Allogeneic mixed lymphocyte reaction Monocytes were enriched from PBMCs (AllCells, Cat# PB002-C-300) by adherence on tissue c | Full Paper |
2022 | McGuire, BE;Mela, JE;Thompson, VC;Cucksey, LR;Stevens, CE;McWhinnie, RL;Winkler, DFH;Pelech, S;Nano, FE; | Escherichia coli recombinant expression of SARS-CoV-2 protein fragments | Microbial cell factories | 35123472 | mall number of serum or plasma samples from positive tested donors and all pre-COVID-19 samples were acquired by purchase (Precision for Medicine, Norton, MA, USA; Innovative Research, Novi, MI, USA; AllCells, Alameda, CA, USA) or by donation (CureIm | Full Paper |
2022 | Li, G;Reid, KM;Spitler, K;Beatty, N;Boucher, J;Davila, ML; | CD3 engagement as a new strategy for allogeneic "off-the-shelf" T cell therapy | Molecular therapy oncolytics | 35317526 | lls was harvested, and 0.45 μm was filtered and cryopreserved for future use. For T cell transduction, healthy donor leukopaks were purchased from Stemcell Technologies (Vancouver, BC, Canada) and ALLCELLS (Alameda, CA). PBMCs were isolated using sta | Full Paper |
2022 | Biscaia, S;Branquinho, MV;Alvites, RD;Fonseca, R;Sousa, AC;Pedrosa, SS;Caseiro, AR;Guedes, F;Patrício, T;Viana, T;Mateus, A;Maurício, AC;Alves, N; | 3D Printed Poly(????-caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on a Composite Preparation by Melt Blending or Solvent Casting Techniques and the Influence of Bioceramic Content on Scaffold Properties | International journal of molecular sciences | 35216432 | ON 4505 equipment in a dry state at a rate of 1 mm/min. Five specimens were tested for each composition. 4.3. Cytocompatibility Analysis 4.3.1. Cell Culture and Maintenance hDPSCs were acquired from AllCells, LLC (Cat. DP0037F, Lot N° DPSC090411-01) | Full Paper |
2022 | Cunningham, AW;Jones, M;Frank, N;Sethi, D;Miller, MM; | Stem-like memory T cells are generated during hollow fiber perfusion-based expansion and enriched after cryopreservation in an automated modular cell therapy manufacturing process | Cytotherapy | 36031522 | Leukopaks from three healthy donors were acquired by processing whole blood on the Spectra Optia Apheresis System and obtained through AllCells (Alameda, CA, USA) (Fig. 1A). The apheresis product was shipped to the lab overnight, and CD3+ T cells wer | Full Paper |
2022 | Jimenez, AC;Heist, CA;Navaei, M;Yeago, C;Roy, K; | Longitudinal two-dimensional gas chromatography mass spectrometry as a non-destructive at-line monitoring tool during cell manufacturing identifies volatile features correlative to cell product quality | Cytotherapy | 35882596 | CellBIND 96-well plate (Corning) for 48-h before co-culture at 50 000 cells per well. PBMCs (AllCells, Alameda, CA, USA) were thawed with a CTL anti-aggregate solution (Cellular Technology Ltd, Shaker Heights, OH, USA) and plated in a six-well plate | Full Paper |
2022 | Maughon, TS;Shen, X;Huang, D;Michael, AOA;Shockey, WA;Andrews, SH;McRae, JM;Platt, MO;Fernández, FM;Edison, AS;Stice, SL;Marklein, RA; | Metabolomics and cytokine profiling of mesenchymal stromal cells identify markers predictive of T-cell suppression | Cytotherapy | 34696960 | ethylenediaminetetraacetic acid; Gibco) and then seeded at three densities (10 000, 5000 and 2000 cells/well) in a 96-well plate and cultured for 24 h. PBMCs (AllCells, Alameda, CA, USA) were thawed in Roswell Park Memorial Institute medium, 20% feta | Full Paper |
2022 | Xu, C;Yang, J;Kosters, A;Babcock, BR;Qiu, P;Ghosn, EEB; | Comprehensive multi-omics single-cell data integration reveals greater heterogeneity in the human immune system | iScience | 36185375 | ical samples ------------------------- Human Adult Peripheral Blood Emory University’s Children’s Clinical and Translational Discovery Core N/A Human Adult Bone Marrow Emory University; AllCells N/A ------------------------- Critical co | Full Paper |
2022 | Magis, W;DeWitt, MA;Wyman, SK;Vu, JT;Heo, SJ;Shao, SJ;Hennig, F;Romero, ZG;Campo-Fernandez, B;Said, S;McNeill, MS;Rettig, GR;Sun, Y;Wang, Y;Behlke, MA;Kohn, DB;Boffelli, D;Walters, MC;Corn, JE;Martin, DIK; | High-level correction of the sickle mutation is amplified in vivo during erythroid differentiation | iScience | 35633935 | Cryopreserved CD34+ cells were thawed according to AllCells instructions and cultured for 2 days in StemSpan SFEM with CC110 supplement (StemCell Technologies). For each Save;. Methods CD34+cells CD34+ HSPCs homozygous for the sickle mutation were | Full Paper |
2022 | Liu, P;Qin, L;Liu, C;Mi, J;Zhang, Q;Wang, S;Zhuang, D;Xu, Q;Chen, W;Guo, J;Wu, X; | Exosomes Derived From Hypoxia-Conditioned Stem Cells of Human Deciduous Exfoliated Teeth Enhance Angiogenesis via the Transfer of let-7f-5p and miR-210-3p | Frontiers in cell and developmental biology | 35557954 | Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Cat. H-001F, AllCells) and were cultured in DMEM (Cat. SH30021.01, Hyclone) supplemented with 10 Save | Full Paper |
2022 | Li, L;Zhou, B;Xu, H;Shi, H;Gao, L;Ge, B; | Zinc-Loaded Black Phosphorus Multifunctional Nanodelivery System Combined With Photothermal Therapy Have the Potential to Treat Prostate Cancer Patients Infected With COVID-19 | Frontiers in endocrinology | 35464050 | n, USA). Human HMGB1 ELISA Kit was obtained from Arigo Biolaboratories (Taiwan, China). ATP content detection kit was obtained from Solarbio (Beijing, China). Dendritic cells (DCs) were obtained from AllCells (San Francisco, USA). FITC anti-human CD1 | Full Paper |
2022 | McGovern, K;Castro, AC;Cavanaugh, J;Coma, S;Walsh, M;Tchaicha, J;Syed, S;Natarajan, P;Manfredi, M;Zhang, XM;Ecsedy, J; | Discovery and Characterization of a Novel Aryl Hydrocarbon Receptor Inhibitor, IK-175, and Its Inhibitory Activity on Tumor Immune Suppression | Molecular cancer therapeutics | 35666806 | Naive human T cells (CD4+/CD62L+) from two independent donors were isolated from cryopreserved CD4+ T cells (AllCells) by magnetic selection with human CD62 L microbeads (Miltenyi). On Day 1, naive CD62L+ human T cells were activated with human CD3/C | Full Paper |
2022 | Lin, W;Qian, J;Wang, H;Ren, L;Yang, Y;Chen, C;Chen, X;Huang, Y;Liu, J;Xu, N;Teng, L; | Cisplatin plus anti-PD-1 antibody enhanced treatment efficacy in advanced esophageal squamous cell carcinoma | American journal of cancer research | 35261780 | Therapies for patients with advanced esophageal squamous cell carcinoma (ESCC) are limited and accompanied by dismal prognosis. Here we use ESCC cell line K30 and TE-1 to Save | Full Paper |
2022 | Duan, J;Lei, D;Ling, C;Wang, Y;Cao, Z;Zhang, M;Zhang, H;You, Z;Yao, Q; | Three-dimensional-printed polycaprolactone scaffolds with interconnected hollow-pipe structures for enhanced bone regeneration | Regenerative biomaterials | 35719204 | CR MasterMix (ABM, CA) on a Light Cycler 480 II (Roche, CHE). To evaluate the expression of angiogenesis genes (HIF1-α and VEGF), human umbilical vein endothelial cells (HUVECs) were purchased from AllCells. First, 6 × 105 HUVECs were seeded vertica | Full Paper |
2022 | Li, Z;Kou, L;Fu, X;Xie, Z;Xu, M;Guo, L;Lin, T;Gong, S;Zhang, S;Liu, M; | Design, synthesis, and biological evaluation of triazole-pyrimidine-methylbenzonitrile derivatives as dual A2A/A2B adenosine receptor antagonists | Journal of enzyme inhibition and medicinal chemistry | 35616298 | Cryopreserved PBMCs (Allcells) were thawed and recovered in RPMI1640 medium overnight before being used. And compounds to be tested were diluted with DMSO to 100 folds of Save | Full Paper |
2022 | Hu, S;Muniraj, G;Mishra, A;Hong, K;Lum, JL;Hong, CHL;Rosa, V;Sriram, G; | Characterization of silver diamine fluoride cytotoxicity using microfluidic tooth-on-a-chip and gingival equivalents | Dental materials : official publication of the Academy of Dental Materials | 35778310 | Objective This study aims to characterize the cytotoxicity potential of silver diamine fluoride (SDF) on dental pulp stem cells (DPSC) and gingival equivalents. Methods DPSC cultured on Save | Full Paper |
2022 | Dogan, M;Karhan, E;Kozhaya, L;Placek, L;Chen, X;Yigit, M;Unutmaz, D; | Engineering Human MAIT Cells with Chimeric Antigen Receptors for Cancer Immunotherapy | Journal of immunology (Baltimore, Md. : 1950) | 36165183 | Healthy adult blood was obtained from AllCells (Quincy, MA). PBMCs were isolated using Ficoll-Paque plus (GE Healthcare). CD4 + T, CD8 + T, and CD19 + B cells and CD14 + Save | Full Paper |
2022 | Ben Nasr, M;Robbins, D;Parone, P;Usuelli, V;Tacke, R;Seelam, AJ;Driver, E;Le, T;Sabouri-Ghomi, M;Guerrettaz, L;Shoemaker, D;Fiorina, P; | Pharmacologically Enhanced Regulatory Hematopoietic Stem Cells Revert Experimental Autoimmune Diabetes and Mitigate Other Autoimmune Disorders | Journal of immunology (Baltimore, Md. : 1950) | 35321879 | A total of 5000 cryopreserved and preisolated human mobilized peripheral blood HSCs (CD34 + cells) (AllCells) were plated into each well of a 384-well plate (Corning) in StemSpan- Save | Full Paper |
2022 | Melani, RD;Des Soye, BJ;Kafader, JO;Forte, E;Hollas, M;Blagojevic, V;Negrão, F;McGee, JP;Drown, B;Lloyd-Jones, C;Seckler, HS;Camarillo, JM;Compton, PD;LeDuc, RD;Early, B;Fellers, RT;Cho, BK;Mattamana, BB;Goo, YA;Thomas, PM;Ash, MK;Bhimalli, PP;Al-Harthi, L;Sha, BE;Schneider, JR;Kelleher, NL; | Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire | Journal of proteome research | 34878788 | odium heparin (BD 367874, Fisher Scientific) by centrifugation at 1,500 × g for 10 min. Plasma from a convalescent patient featuring a high titer of anti-SARS-CoV-2-RBD antibodies was purchased from AllCells (commercial sample 1 - CS1) and used as a | Full Paper |
2022 | Kawato, Y;Fukahori, H;Nakamura, K;Kanno, A;Kubo, K;Hiramitsu, M;Matsuda, T;Hanada, Y;Furukawa, T;Nakajima, Y;Kinugasa, F;Morokata, T; | Potential benefit of the cathepsin S inhibitor, ASP1617, as a treatment for systemic lupus erythematosus | European journal of pharmacology | 35157914 | Five different lots of peripheral blood-derived frozen B cell were purchased from AllCells (Alameda, CA, USA). Cell suspensions (final, 2 × 10 6 Save | Full Paper |
2022 | Pieles, O;Höring, M;Adel, S;Reichert, TE;Liebisch, G;Morsczeck, C; | Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle Cells | Stem cells international | 35903407 | Human dental follicle cells were purchased from AllCells (Alameda, CA, USA) and cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS) Save | Full Paper |
2022 | Schlichtner, S;Yasinska, IM;Ruggiero, S;Berger, SM;Aliu, N;Prunk, M;Kos, J;Meyer, NH;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV; | Expression of the Immune Checkpoint Protein VISTA Is Differentially Regulated by the TGF-β1 - Smad3 Signaling Pathway in Rapidly Proliferating Human Cells and T Lymphocytes | Frontiers in medicine | 35223897 | Medium was also supplemented with penicillin (50 IU/ml), and streptomycin sulfate (50 μg/ml). Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were also purchased from AllCells (Alameda, CA, USA) and handled according to | Full Paper |
2022 | Yoshimaru, K;Yamaza, T;Kajioka, S;Sonoda, S;Yanagi, Y;Matsuura, T;Yoshizumi, J;Oda, Y;Iwata, N;Takai, C;Nakayama, S;Taguchi, T; | Dental pulp stem cells as a therapy for congenital entero-neuropathy | Scientific reports | 35484137 | ies were passaged to expand the number of dDPSCs. Cells at P3 were used for the experiments. BMMSCs isolated from human whole bone marrow aspirates obtained from healthy adult volunteers (n = 3; AllCells, Alameda, CA, USA) and human skin fibroblasts | Full Paper |
2022 | Herrera, VLM;Walkey, AJ;Nguyen, MQ;Gromisch, CM;Mosaddhegi, JZ;Gromisch, MS;Jundi, B;Lukassen, S;Carstensen, S;Denis, R;Belkina, AC;Baron, RM;Pinilla-Vera, M;Mueller, M;Kimberly, WT;Goldstein, JN;Lehmann, I;Shih, AR;Eils, R;Levy, BD;Ruiz-Opazo, N; | A targetable 'rogue' neutrophil-subset, [CD11b+DEspR+] immunotype, is associated with severity and mortality in acute respiratory distress syndrome (ARDS) and COVID-19-ARDS | Scientific reports | 35379853 | ARDS (n = 4) for inertial microfluidic separation (BDL, RMB, MPV) according to respective institutional guidelines. Blood collection Fresh normal peripheral blood neutrophils were procured from AllCells, LLC (CA), which were shipped overnight in Isc | Full Paper |
2022 | Kim, DE;Shin, YH;Cho, JE;Myung, S;Kim, HG;Kim, KC;Park, CM;Yoon, CH; | Identification of 3-Oxindole Derivatives as Small Molecule HIV-1 Inhibitors Targeting Tat-Mediated Viral Transcription | Molecules (Basel, Switzerland) | 35956872 | d fetal bovine serum (all obtained from Gibco-BRL, Gaithersburg, MD, USA). The bl-DTR cells were additionally supplemented with 1 μg/mL puromycin and 200 μg/mL zeocin. The PBMCs were purchased from AllCells (Alameda, CA, USA) and cultured, as describ | Full Paper |
2022 | Saaid, A;Ensanya, A;Suzina, S;Nurul, A;Samsudin, A;Azlina, A; | Odontogenic induction of human amniotic membrane scaffold for dental pulp regeneration | Materials Chemistry and Physics | For this study, deciduous teeth stem cells (DTSCs), obtained from ALLCELLS, USA, were used at passage 4-8. The cells were allowed to grow in a-modified Eagle's medium (α-MEM) supplemented with 20% fetal bovine serum (FBS) and 1% penicillin/streptomyc | Full Paper | |
2022 | Jaimes, MC;Leipold, M;Kraker, G;Amir, EA;Maecker, H;Lannigan, J; | Full spectrum flow cytometry and mass cytometry: A 32-marker panel comparison | Cytometry. Part A : the journal of the International Society for Analytical Cytology | 35593221 | Frozen PBMCs from 5 donors were obtained from AllCells Alameda (Alameda, CA). Cells were provided aliquoted in 25 million cells per vial in freezing media and cryopreserved in liquid nitrogen until ready to use. Ethical review and regulatory complian | Full Paper |
2022 | Moore, KP;Schwaid, AG;Tudor, M;Park, S;Beshore, DC;Converso, A;Shipe, WD;Anand, R;Lan, P;Moningka, R;Rothman, DM;Sun, W;Chi, A;Cornella-Taracido, I;Adam, GC;Bahnck-Teets, C;Carroll, SS;Fay, JF;Goh, SL;Lusen, J;Quan, S;Rodriguez, S;Xu, M;Andrews, CL;Song, C;Filzen, T;Li, J;Hollenstein, K;Klein, DJ;Lammens, A;Lim, UM;Fang, Z;McHale, C;Li, Y;Lu, M;Diamond, TL;Howell, BJ;Zuck, P;Balibar, CJ; | A Phenotypic Screen Identifies Potent DPP9 Inhibitors Capable of Killing HIV-1 Infected Cells | ACS chemical biology | 36044633 | Cells were purchased from AllCells in cryopreserved vials. Cells were centrifuged at 500×g for 10 min at 4 C. The supernatant was removed, and cells were washed with 20 mL PBS. Save | Full Paper |
2022 | Zhang, H;Lv, X;Kong, Q;Tan, Y; | IL-6/IFN-γ double knockdown CAR-T cells reduce the release of multiple cytokines from PBMCs in vitro | Human vaccines & immunotherapeutics | 35049413 | rward TGGGAGCATGTGAATGCCATCC Reverse GTCGGCTCCTGGAGGTCAAACA β-actin Forward CGCCCCAGGCACCAGGGC Reverse GCTGGGGTGTTGAAGGT 2.3. IL-6/IFN-γ double KD CAR-T cell culture The PBMCs (Allcells, Cat: PB005F) were resuspended in CTSTM AIM | Full Paper |
2022 | Zhou, Z;Qin, M;Khodahemmati, S;Li, W;Niu, B;Li, J;Liu, Y;Gao, J; | Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis | International journal of environmental health research | 34102927 | We maintained HUVECs (Allcells, China) routinely in HUVEC complete culture medium (Allcells, China), supplemented with 10% fetal bovine serum (FBS), 2% growth factors, 100 U/mL Save | Full Paper |
2022 | Wang, X;Wong, LM;McElvain, ME;Martire, S;Lee, WH;Li, CZ;Fisher, FA;Maheshwari, RL;Wu, ML;Imun, MC;Murad, R;Toledo Warshaviak, D;Yin, J;Kamb, A;Xu, H; | A rational approach to assess off-target reactivity of a dual-signal integrator for T cell therapy | Toxicology and applied pharmacology | 35085592 | Informed-consent for use of primary T cells and donor collection protocols was approved by an Institutional Review Board (IRB) at Allcells or HemaCare. Allcells and HemaCare Save | Full Paper |
2022 | Andrews, SH;Klinker, MW;Bauer, SR;Marklein, RA; | Morphological landscapes from high content imaging reveal cytokine priming strategies that enhance mesenchymal stromal cell immunosuppression | Biotechnology and bioengineering | 34716713 | Human bone marrow-derived MSCs were obtained from four different donors purchased from Lonza, AllCells, and RoosterBio (see Table S1 for donor information). MSC culture conditions for the Lonza and AllCells (8F3560, 110877, PCBM1662) were chosen base | Full Paper |
2022 | Mandal, S;Sunagawa, SW;Prathipati, PK;Belshan, M;Shibata, A;Destache, CJ; | Targeted Immuno-Antiretroviral to Promote Dual Protection against HIV: A Proof-of-Concept Study | Nanomaterials (Basel, Switzerland) | 35683795 | were maintained in a complete DMEM medium (HiDMEM medium supplemented with 10% FBS and 1× AA) as standard protocol [14,15]. The human peripheral blood mononuclear cells (hPBMCs) were purchased from AllCells (Alameda, CA, USA) and maintained in comp | Full Paper |
2022 | Huang, P;Zhang, C;Delawary, M;Korchak, JA;Suda, K;Zubair, AC; | Development and evaluation of IL-6 overexpressing mesenchymal stem cells (MSCs) | Journal of tissue engineering and regenerative medicine | 34890489 | Human bone marrow derived mesenchymal stem cells (BMSCs) were isolated from commercial de-identified bone marrow (AllCells, Alameda, CA) from a healthy 28-year-old male Save | Full Paper |
2022 | Harper, T;Sharma, A;Kaliyaperumal, S;Fajardo, F;Hsu, K;Liu, L;Davies, R;Wei, YL;Zhan, J;Estrada, J;Kvesic, M;Nahrwold, L;Deisting, W;Panzer, M;Cooke, K;Lebrec, H;Nolan-Stevaux, O; | Characterization of an Anti-CD70 Half-Life Extended Bispecific T-Cell Engager (HLE-BiTE) and Associated On-Target Toxicity in Cynomolgus Monkeys | Toxicological sciences : an official journal of the Society of Toxicology | 35583313 | Human T cells (AllCells) or cynomolgus monkey or human peripheral blood mononuclear cells (PBMCs) (SNBL) were cocultured with target cells at an effector cell (E) to target cell (T) Save | Full Paper |
2022 | Wang, P;Zhang, J;Zhang, H;Zhang, F; | The role of MACF1 on acute myeloid leukemia cell proliferation is involved in Runx2-targeted PI3K/Akt signaling | Molecular and cellular biochemistry | 35857251 | Bone marrow CD34 + hematopoietic stem and progenitor cells (HSPCs) were purchased from AllCells (Alameda, CA, USA) and cultured in X-Vivo 15 media (Lonza, Basel, Switzerland) Save | Full Paper |
2022 | Shams, F;Bayat, H;Mohammadian, O;Mahboudi, S;Vahidnezhad, H;Soosanabadi, M;Rahimpour, A; | Advance trends in targeting homology-directed repair for accurate gene editing: An inclusive review of small molecules and modified CRISPR-Cas9 systems | BioImpacts : BI | 35975201 | A) Treatment group 1A (unedited HSCs from AllCells). The first mouse was found dead in Treatment group 2B, which contained edited bought HSCs (AllCells), had the highest bone Save | Full Paper |
2022 | Liu, Y;Ma, X;Yang, H;Li, X;Ma, Y;Ason, B;Liu, S;Hu, LA; | APLNR Regulates IFN-γ signaling via β-arrestin 1 mediated JAK-STAT1 pathway in melanoma cells | The Biochemical journal | 35084016 | Naive CD8+ T cells used for co-culture assay were obtained from Allcells and cultured in RPMI 1640 medium supplemented with 10% FBS, 1× HEPES, 1× PS, 2 mM Glutamine, 1 mM Save | Full Paper |
2022 | Venn-Watson, SK;Butterworth, CN; | Broader and safer clinically-relevant activities of pentadecanoic acid compared to omega-3: Evaluation of an emerging essential fatty acid across twelve primary human cell-based disease systems | PloS one | 35617322 | lowed ethical guidelines and documented informed consent: Cell Applications, Inc., CellzDirect, Celsis-IVT, Leukolab, Life Technologies, Lonza, ScienCell, Hemacare Corporation, Stemcell Technologies, AllCells, Physician’s Plasma Alliance, Lifeline Ce | Full Paper |
2022 | Okada, Y;Kawashima, N;Noda, S;Murano, H;Han, P;Hashimoto, K;Kaneko, T;Okiji, T; | VEGFA promotes odonto/osteoblastic differentiation in dental pulp stem cells via ERK/p38 signaling | Journal of Dental Sciences | SHEDs (P5, DP001F; AllCells, Alameda, CA, USA) were cultured in alpha-modified Eagle's minimum essential medium (α-MEM) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and an antibiotic and antifungal soluti | Full Paper | |
2022 | Chen, W;Qie, C;Hu, X;Wang, L;Jiang, J;Liu, W;Liu, J; | A small molecule inhibitor of VSIG-8 prevents its binding to VISTA | Investigational new drugs | 35404016 | PBMCs were purchased from Allcells Biotechnology (Shanghai) Co., LTD, and we got a statement confirming that informed consent was obtained from all subjects, the attachment is on response. Jurkat cells were cultured in RPMI-1640 medium (Biological In | Full Paper |
2022 | Han, Y;Bai, X;Wang, X; | Exosomal myeloperoxidase as a biomarker of deep venous thrombosis | Annals of translational medicine | 35242854 | al microscope (Olympus). HUVECs culture We purchased HUVECs from American Type Culture Collection and cultured them in endothelial cell complete medium containing endothelial cell growth supplement (AllCells, Alameda, CA, USA) at 37 °C in a humidifi | Full Paper |
2022 | Sato, M;Fujii, K;Takagi, H;Shibuya, I;Oka, D;Yamaya, N;Hagita, H;Matsumoto, M;Inagaki, K; | Antibacterial and Immunosuppressive Effects of OPS-2071, a Candidate Therapy for Inflammatory Bowel Disease | Digestive diseases and sciences | 34463880 | Mouse splenocytes were prepared from eight-week-old female BALB/c mice, and human peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Alameda, CA). To Save | Full Paper |
2022 | Skaddan, MB;Wooten, DW;Wilcox, KC;Voorbach, MJ;Reuter, DR;Jia, ZJ;Foster-Duke, KD;Hickson, JA;Vaidyanathan, S;Reed, AD;Tovcimak, AE;Guo, Q;Comley, RA;Lee, L;Finnema, SJ;Mudd, SR; | [18F]BTK-1: A Novel Positron Emission Tomography Tracer for Imaging Bruton's Tyrosine Kinase | Molecular imaging and biology | 35482146 | Purpose Bruton's tyrosine kinase (BTK) is a key component of B cell receptor (BCR) signaling, and as such a critical regulator of cell proliferation and survival. Aberrant BCR signaling is Save | Full Paper |
2022 | Wu, C;Wang, X;Shang, H;Wei, H; | Construction of a Humanized PBMC-PDX Model to Study the Efficacy of a Bacterial Marker in Lung Cancer Immunotherapy | Disease markers | 36072895 | , Ltd., and the RPMI-1640 Complete Medium (10%FBS; Gibco) was used for culture. When the cells reached 80% confluence, they were passed on. 2.3. PBMCs Immune Reconstitution PBMCs were purchased from AllCells and processed according to the company's | Full Paper |
2022 | Ryan, DE;Diamant-Levi, T;Steinfeld, I;Taussig, D;Visal-Shah, S;Thakker, S;Lunstad, BD;Kaiser, RJ;McCaffrey, R;Ortiz, M;Townsend, J;Welch, WRW;Singh, M;Curry, B;Dellinger, DJ;Bruhn, L; | Phosphonoacetate Modifications Enhance the Stability and Editing Yields of Guide RNAs for Cas9 Editors | Biochemistry | 35436085 | Human primary T cells (LP, CR, CD3+, NS) were obtained from AllCells (Alameda, CA) and were cultured in RPMI 1640 plus GlutaMax media supplemented with 10% fetal bovine serum, 5 ng/mL human IL-7, and 5 ng/mL human IL-15 (Gibco). Primary T cells were | Full Paper |
2022 | Darbaniyan, F;Zheng, H;Kanagal-Shamanna, R;Lockyer, P;Montalban-Bravo, G;Estecio, M;Lu, Y;Soltysiak, KA;Chien, KS;Yang, H;Sasaki, K;Class, C;Ganan-Gomez, I;Do, KA;Garcia-Manero, G;Wei, Y; | Transcriptomic Signatures of Hypomethylating Agent Failure in Myelodysplastic Syndromes and Chronic Myelomonocytic Leukemia | Experimental hematology | 36150563 | CD34 + cells isolated from healthy individuals (AllCells, Alameda CA) and from patients with MDS or CMML were plated into human methylcellulose colony formation medium Methocult- Save | Full Paper |
2022 | Valdez, BC;Yuan, B;Murray, D;Nieto, Y;Popat, U;Andersson, BS; | Enhanced cytotoxicity of bisantrene when combined with venetoclax, panobinostat, decitabine and olaparib in acute myeloid leukemia cells | Leukemia & lymphoma | 35188042 | Bisantrene (Bis), a topoisomerase-II inhibitor, is less cardiotoxic than the current anthracyclines. Its synergistic cytotoxicity with newly developed antineoplastic drugs has not been Save | Full Paper |
2022 | Fan, F;Liu, F;Shen, P;Tao, L;Zhang, H;Wu, H; | Salvianolic acid B, a new type I IRE1 kinase inhibitor, abrogates AngII-induced angiogenesis by interacting with IRE1 in its active conformation | Clinical and experimental pharmacology & physiology | 36153795 | Primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China) and cultured in EBM media (AllCells ) according to the manufacturer's Save | Full Paper |
2022 | Tong, Q;Liu, H;Qi, Q;Dai, C;Yang, T;Qian, F; | Development of a fully human anti-GITR antibody with potent antitumor activity using H2L2 mice | FEBS open bio | 35674216 | h well, and luminescence values were measured using a Varioskan LUX (Thermo, Carlsbad, CA, USA). T‐cell activation assays Total T cells from frozen human peripheral blood mononuclear cells (PBMCs, AllCells, Alameda, CA, USA) were first isolated usin | Full Paper |
2022 | Yang, M;Tkach, D;Boyne, A;Kazancioglu, S;Duclert, A;Poirot, L;Duchateau, P;Juillerat, A; | Optimized two-step electroporation process to achieve efficient nonviral-mediated gene insertion into primary T cells | FEBS open bio | 34510816 | ineering in human primary cells and provide a foundation for further advances using nonviral gene editing methods. Materials and methods T‐cell culture Cryopreserved human PBMCs were acquired from ALLCELLS. PBMCs were cultured in X‐vivo‐15 media (Lo | Full Paper |
2022 | Pieles, O;Reichert, TE;Morsczeck, C; | Protein kinase A is activated during bone morphogenetic protein 2-induced osteogenic differentiation of dental follicle stem cells via endogenous parathyroid hormone-related protein | Archives of oral biology | 35338829 | Human dental follicle stem cells (Catalog number FT002F, Lot number ACF001) were commercially obtained from AllCells (Emeryville, USA) and cultured in Dulbecco's Modified Eagle's Medium (DMEM) high glucose (Sigma Aldrich, St. Louis, USA) with 10% fet | Full Paper |
2022 | Qian, J;Yu, J;Zhu, X;Liang, S; | MiR-335 promotes corneal neovascularization by Targeting EGFR | BMC ophthalmology | 35701740 | surgery and on day 3, 7, 11 and 14. The Animal Research Committee of the Affiliated Hospital of Nantong University approved all animal studies. Cell culture and different treatments HUVECs were from AllCells (Shanghai, China). They were put in an RP | Full Paper |
2022 | Wang, W;Tang, W;Shan, E;Zhang, L;Chen, S;Yu, C;Gao, Y; | MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FAS | European journal of histochemistry : EJH | 35638591 | cells (HUVEC) in vitro. Materials and Methods Cell culture HUVEC cells have played a major role as a model system for the study of the regulation of endothelial cell function. HUVEC cells (Shanghai AllCells Biotech Co., Ltd. Shanghai, China) were cu | Full Paper |
2022 | Koh, H;Zhen, X;Kim, J;Ha, HY;Lee, JH; | Generation and characterization of human umbilical cord blood-derived induced pluripotent stem cells (KRIBBi005-A) | Stem cell research | 35085946 | Ethical approval Approval obtained by Public Institutional Bioethics Committee designated by the MOHW (P01-201909-31-002). Cord blood mononuclear cells were purchased (AllCells LLC) | Full Paper |
2022 | Hashim, SNM;Azlina, A; | Ultrastructural morphological changes of VEGF treated-stem cells from human exfoliated deciduous teeth cultured on human amniotic membrane | Acta Microscopica | SHED (AllCells, USA) were cultured in a complete medium made of the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, USA), supplemented with 15% of fetal bovine serum (FBS) (Gibco, USA) and 50 U/ml of penicillin-st | Full Paper | |
2022 | Bai, Y;Zhang, X;Zheng, J;Liu, Z;Yang, Z;Zhang, X; | Overcoming high level adenosine-mediated immunosuppression by DZD2269, a potent and selective A2aR antagonist | Journal of experimental & clinical cancer research : CR | 36229853 | bR, CHO-A1R and CHO-A3R) were purchased from GenScript. Cell lines RM-1, B16F10 and Pan02 were obtained from the American Type Culture Collection (ATCC). Cryopreserved human PBMCs were purchased from AllCells. All CHO cells were cultured in Ham’s F12 | Full Paper |
2022 | Kartha, VK;Duarte, FM;Hu, Y;Ma, S;Chew, JG;Lareau, CA;Earl, A;Burkett, ZD;Kohlway, AS;Lebofsky, R;Buenrostro, JD; | Functional inference of gene regulation using single-cell multi-omics | Cell genomics | 36204155 | AILS Human peripheral blood mononuclear cells Cryopreserved human peripheral blood mononuclear cells (PBMCs) and isolated peripheral blood CD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from AllCells (see Table S1 for catalog numbers and don | Full Paper |
2022 | Riaz, S;Azlina, A;Mahmood, Z;Htun, AT; | Long-term treatment of dentine with triple antibiotic paste promotes stem cell viability and attachment | Journal of Taibah University Medical Sciences | 35983454 | , the dentine chips were removed from the well plates and air-dried on a filter paper. The samples were then stored in a sterile container at 4 °C until further use. Cell culture Commercial DPSCs (AllCells, USA) were used from passage 7 to 9 at 70%- | Full Paper |
2022 | Salam, N;Gibson, IR; | Lithium ion doped carbonated hydroxyapatite compositions: Synthesis, physicochemical characterisation and effect on osteogenic response in vitro | Biomaterials advances | 35939955 | the American Type Culture Collection (via LGC standards, UK), PromoCell (Germany), and AllCells (USA), respectively. Cells were maintained in routine culture conditions using: high glucose DMEM with 10 % FBS and 4 mM l-glutamine for C2C12 cells, Gibc | Full Paper |
2022 | Nakauchi, Y;Azizi, A;Thomas, D;Corces, MR;Reinisch, A;Sharma, R;Cruz Hernandez, D;Köhnke, T;Karigane, D;Fan, A;Martinez-Krams, D;Stafford, M;Kaur, S;Dutta, R;Phan, P;Ediriwickrema, A;McCarthy, E;Ning, Y;Phillips, T;Ellison, CK;Guler, GD;Bergamaschi, A;Ku, CJ;Levy, S;Majeti, R; | The Cell Type-Specific 5hmC Landscape and Dynamics of Healthy Human Hematopoiesis and TET2-Mutant Preleukemia | Blood cancer discovery | 35532363 | Normal donor human BM cells were obtained fresh from AllCells, and peripheral blood cells Human BM samples (CD34+ cell enriched) were purchased from AllCells. Mononuclear Save | Full Paper |
2022 | Baskar, R;Chen, AF;Favaro, P;Reynolds, W;Mueller, F;Borges, L;Jiang, S;Park, HS;Kool, ET;Greenleaf, WJ;Bendall, SC; | Integrating transcription-factor abundance with chromatin accessibility in human erythroid lineage commitment | Cell reports methods | 35463156 | # ab181544 Cleaved caspase 3-PE BD Biosciences Cat# 550821, RRID:AB_393906 ------------------------- Biological samples ------------------------- Adult bone marrow All Cells Inc https://allcells.com/research-grade-tissue-products/bone-m | Full Paper |
2022 | Kwak, J;Choi, W;Bae, Y;Kim, M;Choi, S;Oh, W;Jin, H; | HLA-A2 Promotes the Therapeutic Effect of Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Hyperoxic Lung Injury | Bioengineering (Basel, Switzerland) | 35447737 | St. Louis, MO, USA) for 1 h at 37 °C. The UCB-MSCs (1 × 103 cells/well) were seeded and maintained at 37 °C in a humidified incubator for 4 h and then co-cultured with PBMCs (1 × 105 cells/well; Allcells, Boston, MA, USA) from different donors. Phyto | Full Paper |
2022 | Vivanco Gonzalez, N;Oliveria, JP;Tebaykin, D;Ivison, GT;Mukai, K;Tsai, MM;Borges, L;Nadeau, KC;Galli, SJ;Tsai, AG;Bendall, SC; | An optimized protocol for phenotyping human granulocytes by mass cytometry | STAR protocols | 35434655 | y donors (with unknown allergy status) were obtained from the Stanford Blood Center (Palo Alto, CA, USA). Healthy bone marrow and peripheral blood samples paired from the same donor were ordered from AllCells (Alameda, CA, USA). Samples from patients | Full Paper |
2022 | Bell-Glenn, S;Thompson, JA;Salas, LA;Koestler, DC; | A Novel Framework for the Identification of Reference DNA Methylation Libraries for Reference-Based Deconvolution of Cellular Mixtures | Frontiers in bioinformatics | 35419567 | Cell Mixture Reconstruction Experiment Purified cells taken from normal human subjects were purchased from AllCells LLC (Emeryville, CA). Namely, granulocytes, monocytes, CD4T, Save | Full Paper |
2022 | Shui, S;Wang, S;Liu, J; | Systematic Investigation of the Effects of Multiple SV40 Nuclear Localization Signal Fusion on the Genome Editing Activity of Purified SpCas9 | Bioengineering (Basel, Switzerland) | 35200436 | s medium (DMEM) supplemented with 10% (v/v) FBS, 100 U/mL penicillin and 100 U/mL streptomycin. The medium of EGFP reporter contained an additional 50 U/mL hygromycin. Human dermal fibroblasts (hDF, AllCells) were acquired from Cell Applications (Lot | Full Paper |
2022 | Li, L;Xu, H;Gao, L; | Doxorubicin-Loaded Black Phosphorus Multifunctional Nanodelivery System Combined with Photothermal Therapy Promotes Immunogenic Death of Prostate Cancer PC-3 Cells | SSRN Electronic Journal | Beijing, China). Dendritic cells (DCs) were 197obtained from AllCells (San Francisco, USA). FITC anti198human CD11c Antibody, Alexa Fluor® 647 anti-human 199CD80 Antibody, PE anti-human CD86 Antibody, and 200PerCP/Cyanine5.5 anti-human HLA-DR Antibod | Full Paper | |
2022 | Calderon, A;Kim, Y;Favaro, P;Baskar, R;Valainis, J;Reynolds, W;Borges, L;Glass, D;Tsai, A;Bendall, S; | Multi-Omic Single Cell Identification of Lin- CD34- Natural Killer Cell Progenitors in Human Hematopoiesis | SSRN Electronic Journal | Deidentified human blood (n=5) and BM (n=7) were obtained from healthy adult donors (Stanford Blood Center, Allcells). All samples were obtained under informed consent and in Save | Full Paper | |
2022 | Lee, J;Jonus, H;Sadanand, A;Branella, G;Maximov, V;Suttapitugsakul, S;Schniederjan, M;Shim, J;Parwani, K;Fedanov, A;Pilgrim, A;Silva, J;Doering, C;Wu, R;Spencer, H;Goldsmith, K; | Identification and Targeting of Protein Tyrosine Kinase 7 (PTK7) as an Immunotherapy Candidate for Neuroblastoma Cellular Therapy | SSRN Electronic Journal | GD2-targeted immunotherapy improves survival for pediatric patients with neuroblastoma (NB). However, toxicities associated with on-target, off-tumor activity occur. Furthermore, GD2 Save | Full Paper | |
2022 | O'Brien, G;Cecotka, A;Manola, K;Pagoni, M;Polańska, J;Badie, C; | Epigenetic Signature of Ionising Radiation In Therapy-Related AML Patients | SSRN Electronic Journal | Bone marrow aspirate samples were also obtained from 5 healthy normal donors (4 males, 1 female, age 21-33) from AllCells® (Alamada, USA). The project was in accordance with the declaration of Helsinki. Informed consent was provided from patients and | Full Paper | |
2022 | Qian, T;Qi, B;Fei, Y;Li, J;Luo, L;Song, Y;Sheng, S;Lv, B;Huang, X;Wang, X; | Deletion of PLD2 Alleviates LPS Induced Acute Lung Injury by Inhibiting STAT3 Phosphorylation and Regulating Endothelial Tight Junctions | Research Square | HUVECs were purchased from the Shanghai cell bank and cultured in a 5% CO2 incubator at 37oC in a complete culture medium (AllCells, Shanghai, China). Cells were cultured to 85- Save | Full Paper | |
2022 | Li, W;Li, J;Hu, X;Xu, L;Liu, X;Qian, Z;Jin, L;Zhang, Y;Wei, J;Liu, X; | Tumor suppression by Chinese cordyceps extract via anti-angiogenesis | Research Square | Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in special medium for HUVEC cells at 37C with 5% CO2. CCE was Save | Full Paper | |
2022 | Li, L;Chen, X;Li, Y;Lou, L; | Pharmacological characterization of GR1803, a novel BCMA × CD3 bispecific antibody for multiple myeloma treatment | Research Square | The MM cell lines NCI-H929, MM.1S, RPMI-8226, and U266 were obtained from the American Type Culture Collection (Manassas, VA, USA). Fresh peripheral blood mononuclear cells (PBMCs) were obtained from AllCells Co. (Shanghai, China). Natural killer (NK | Full Paper | |
2022 | Yan, F;Luo, B;Chen, C;Tan, B;Cui, D;Wang, M;Qian, J; | Inhibiting PHD2 in bone marrow mesenchymal stem cells in an inflammatory microenvironment facilitates periodontal repair in SD rats | Research Square | Primary BMMSCs of SD rats were isolated and puri ed by AllCells (Alameda, CA, USA), and their phenotype was identi ed. The cells were recovered and cultured in low glucose Save | Full Paper | |
2022 | Guedes, F;Branquinho, M;Biscaia, S;Alvites, R;Sousa, A;Lopes, B;Sousa, P;Rêma, A;Amorim, I;Faria, F;Patrício, T;Bugalho, A;Alves, N;Maurício, A; | Gamma Irradiation Processing on 3D PCL Devices - A Preliminary Biocompatibility Assessment | preprints | Human Dental Pulp stem/stromal cells (hDPSCs) obtained from AllCells, LLC (Cat. DP0037F, Lot Nº DPSC090411-01) were maintained in MEM α, GlutaMAX™ Supplement, no nucleosides (Gibco, 32561029), supplemented with 10% (v/v) fetal bovine serum (FBS) (Gib | Full Paper | |
2022 | Tokatlian, T;Asuelime, G;Naradikian, M;Mock, J;Daris, M;Martin, A;Toledo Warshaviak, D;Kamb, A;Hamburger, A; | Chimeric Antigen Receptors Directed at Mutant KRAS Exhibit an Inverse Relationship Between Functional Potency and Neoantigen Selectivity | Cancer Research Communications | Peripheral blood mononuclear cells (PBMC) from healthy donors were obtained from AllCells. PBMC collection and donor written informed consent were approved by an Institutional Save | Full Paper | |
2022 | Boccacci, Y;Dumont, N;Doyon, Y;Laganière, J; | Accessory-cell-free differentiation of hematopoietic stem and progenitor cells into mature red blood cells | bioRxiv | The culture and ex vivo engineering of red blood cells (RBCs) can help characterize genetic variants, model diseases, and may eventually spur the development of applications in Save | Full Paper | |
2022 | Checkley, M;Luttge, B;Dobrowolski, C;Wald, D;McMahon, D;Haidar, G;Sobolewski, M;Enick, P;Cyktor, J;Mellors, J;Karn, J; | Reduction of the HIV-1 reservoir in T cells from persons with HIV-1 on suppressive antiretroviral therapy using expanded natural killer cells ex vivo | bioRxiv | For this study leukapheresis packs from 5 HIV-1-negative donors were purchased from AllCells. 437 For HIV+ participants, PBMCs from well-suppressed ART-treated patients from the Save | Full Paper | |
2022 | Shabaneh, T;Moffett, H;Stull, S;Derezes, T;Tait, L;Park, S;Riddell, S;Lajoie, M; | Safety switch optimization enhances antibody-mediated elimination of CAR T cells | bioRxiv | Natural killer cells were isolated from cryopreserved, T cell depleted (CD4-/CD8-) PBMC (AllCells) by negative selection using the EasySep Human NK Cell Kit (Stemcell) according to the manufacturer’s protocol. To activate their cytolytic function, is | Full Paper | |
2022 | Wisdom, K;Suijker, J;van den Broek, L;Sridharan, B;Grandhi, T;Cheng, A;Lamb, M;Titus, S;Gehman, A;Poore, D;Shah, N;Cheng, S;Kim, E;Griffin, S;Ekert, J; | Lung Tumor Microphysiological System with 3D Endothelium to Evaluate Modulators of T-Cell Infiltration | bioRxiv | AllCells and shipped to the MIMETAS research facility, or they 653 were isolated from AllCells For this, T-cells were isolated from full 654 fresh leukopaks (AllCells). Leukopaks were Save | Full Paper | |
2022 | Dogan, M;Karhan, E;Kozhaya, L;Placek, L;Chen, X;Yigit, M;Unutmaz, D; | Engineering human Mucosal Associated Invariant T (MAIT) cells with chimeric antigen receptors for cancer immunotherapy1 | bioRxiv | Healthy adult blood was obtained from AllCells Quincy, MA. PBMCs were isolated using Ficoll- 100 paque plus (GE Health care). CD4+ T, CD8+ T, CD19+ B cells, CD14+ monocytes Save | Full Paper | |
2022 | Swift, M;Horns, F;Quake, S; | Fate Bias and Transcriptional Memory of human B cells | bioRxiv | from a healthy male aged 50-55 were obtained from AllCells. Mononuclear cells were isolated using Ficoll gradient and Red Blood Cell Lysis. Plasma cells from the bone marrow were isolated using StemCell EasySepEasySep™ Human CD138 Positive Selection | Full Paper | |
2022 | White, B;de Reyniès, A;Newman, A;Waterfall, J;Lamb, A;Petitprez, F;Valdeolivas, A;Lin, Y;Li, H;Xiao, X;Wang, S;Zheng, F;Yang, W;Yu, R;Guerrero-Gimenez, M;Catania, C;Lang, B;Domanskyi, S;Bertus, T;Piermarocchi, C;Monaco, G;Caruso, F;Ceccarelli, M;Yu, T;Guo, X;Coller, J;Maecker, H;Duault, C;Shokoohi, V;Patel, S;Liliental, J;Simon, S;Saez-Rodriguez, J;Heiser, L;Guinney, J;Gentles, A;Tumor Deconvolution DREAM Challenge consortium, ; | Community assessment of methods to deconvolve cellular composition from bulk gene expression | bioRxiv | Immune and stromal cells provided by StemExpress and AllCells were isolated according to ) and the rest assigned immune cells from AllCells wherever availability allowed (Tables S5 Save Cached | Full Paper | |
2022 | Simmons, S;Lithwick-Yanai, G;Adiconis, X;Oberstrass, F;Iremadze, N;Geiger-Schuller, K;Thakore, P;Frangieh, C;Barad, O;Almogy, G;Rozenblatt-Rosen, O;Regev, A;Lipson, D;Levin, J; | Single cell RNA-seq by mostly-natural sequencing by synthesis | bioRxiv | We purchased 9 cryopreserved human PMBC samples (AllCells). We thawed PBMC vials in a 37C water bath for ∼2 minutes. A quick counting revealed high viability (>90%) in all Save | Full Paper | |
2022 | Seenappa, L;Jakubowski, A;Steinbuck, M;Palmer, E;Haqq, C;Carter, C;Fontenot, J;Villinger, F;McNeil, L;DeMuth, P; | Programming the lymph node immune response with Amphiphile-CpG induces potent cellular and humoral immunity following COVID-19 subunit vaccination in mice and non-human primates | bioRxiv | who had recovered from SARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and ALLCELLS (Alameda, CA). All samples were received and stored frozen at −80°C until analysis | Full Paper | |
2022 | Lutter, L;ter Linde, J;Brand, E;van Konijnenburg, D;Roosenboom, B;Talabur-Horje, C;Oldenburg, B;van Wijk, F; | Compartment-driven imprinting of intestinal CD4 (regulatory) T cells in inflammatory bowel disease and homeostasis | bioRxiv | Cluster 1 (18.6% of allcells) was characterized by KLF2, SELL, CCR7, TCF7 and LEF1, and thus resembled recently migrated/recirculating T cells (figure 4A). This cluster contained both intraepithelial and lamina propria T cells (figure 4E). Similarly, | Full Paper | |
2022 | Jaffe, D;Shahi, P;Adams, B;Chrisman, A;Finnegan, P;Raman, N;Royall, A;Tsai, F;Vollbrecht, T;Reyes, D;McDonnell, W; | Functional antibodies exhibit light chain coherence | bioRxiv | We titrated and developed the B cell fractionation panel using 20 million fresh PBMCs (AllCells, catalog # 3050363) from a healthy human donor whose cells were not used to generate Save | Full Paper | |
2022 | Persad, S;Choo, Z;Dien, C;Masilionis, I;Chaligné, R;Nawy, T;Brown, C;Pe’er, I;Setty, M;Pe’er, D; | SEACells: Inference of transcriptional and epigenomic cellular states from single-cell genomics data | bioRxiv | Cryopreserved bone marrow stem/progenitor CD34+ cells from a healthy donor were purchased from AllCells, LLC. (catalog no. ABM022F) and stored in vapor phase nitrogen. Vial was removed from the storage and immediately thawed at 37 °C in a water bath | Full Paper | |
2022 | Stuart, T;Hao, S;Zhang, B;Mekerishvili, L;Landau, D;Maniatis, S;Satija, R;Raimondi, I; | Nanobody-tethered transposition allows for multifactorial chromatin profiling at single-cell resolution | bioRxiv | Cryopreserved healthy donor PBMCs were isolated from mobilized peripheral blood, BMMCs were purchased from AllCells. After thawing into DMEM with 10% FBS, the cells were spun Save | Full Paper | |
2022 | MacMullan, M;Dunn, Z;Qu, Y;Wang, P;Graham, N; | Phospho-proteomic analysis of CAR-T cell signaling following activation by antigen-presenting cancer cells | bioRxiv | cDNAs from BM CD34+, PB CD34+, BM MNCs, PB MNCs and BM MSCs purchased from AllCells (Emeryville, CA, USA) was obtained from a population of positive cells (purity exceeding 95%) collected from five individual donors. The level of cognate cDNA was mea | Full Paper | |
2022 | Dogan, M;Kozhaya, L;Placek, L;Karabacak, F;Yigit, M;Unutmaz, D; | Targeting SARS-CoV-2 infection through CAR-T like bispecific T cell engagers incorporating ACE2 | bioRxiv | Healthy adult blood was obtained from AllCells. PBMCs were isolated using Ficoll-paque plus (GE Health care). CD8 T cells were purified using Dynal CD8 Positive Isolation Kit (from Save | Full Paper | |
2022 | Yashar, W;Curtiss, B;Coleman, D;Van-Campen, J;Kong, G;Macaraeg, J;Estabrook, J;Demir, E;Long, N;Bottomly, D;McWeeney, S;Tyner, J;Druker, B;Maxson, J;Braun, T; | Disruption of the MYC Super-Enhancer Complex by Dual Targeting of FLT3 and LSD1 in Acute Myeloid Leukemia | bioRxiv | Whole bone marrow was obtained (AllCells) and CD34+ cells were selected using CD34 MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions. For the colony assay, 500 CD34+ cells were used per replicate and plated in MethoCult™ H4435 | Full Paper | |
2022 | Aksöz, M;Gafencu, G;Stoilova, B;Buono, M;Meng, Y;Jakobsen, N;Metzner, M;Clark, S;Beveridge, R;Thongjuea, S;Vyas, P;Nerlov, C; | Identification and Age-dependent Increase of Platelet Biased Human Hematopoietic Stem Cells | bioRxiv | Fresh BM from healthy young volunteers (21 to 34 years old males) were purchased from AllCells (Berkeley, CA) or Lonza (Lonza Bioscience, Basel, Switzerland). Bone marrow from older donors was obtained from orthoplastic surgery after | Full Paper | |
2022 | Xiang Ping, M;Zhi, H;Aziz, N;Hadri, N;Ghazalli, N;Yusop, N; | Optimization of agarose-alginate hydrogel bead components for encapsulation and transportation of stem cells | Journal of Taibah University Medical Sciences | Stem cells from human exfoliated deciduous teeth (SHED) purchased from AllCells USA were revived from the cryovial and maintained in culture before encapsulation. SHED from Save | Full Paper | |
2022 | Marcisak, EF; | Leveraging single-cell genomics to uncover clinical and preclinical responses to cancer immunotherapy | Thesis | GFP due to transduction with pBMN-IRES-EGFP containing the FcγRIIIA construct. All NK cell lines were cultured as previously described[231]. Fresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selected | Full Paper | |
2022 | Nesterenko, PA; | Characterizing the T cell immune response through the receptor-ligand interaction | Thesis | Cell culture: Cryo preserved peripheral blood mononuclear cells (PBMCs) were commercially purchased (Allcells and Hemacare). PBMCs were thawed in a water bath set to 37C, transferred to 50 mL conical tube, 1 mL of warm R10 media was added drop wise a | Full Paper | |
2022 | Chu, J; | Ligament tissue engineering: assessing differentiation potential of dental follicle cells in a self-assembly three-dimensional organoid model. | Thesis | according to AllCells company regulations, further donor information such gender and exact age was not provided to the purchaser). The PDL-hTERT immortal cell line has already Save | Full Paper | |
2022 | Plasek, LM; | Self-Silencing: An HIV Induced Mechanism for T Cell Quiescence and HIV Latency | Thesis | Peripheral blood mononuclear cells from HIV negative donors were purchased from AllCells. Naive CD4+ T cells were isolated using the EasySepTM Human Naive CD4+ T Cell Save | Full Paper | |
2022 | BOSYKH, D; | Mechanisms and Biological Consequences of Mutual Reprogramming by Melanoma Cells and Normal Dermal Fibroblasts | Thesis | Cell lines and medium Primary human neonatal dermal fibroblasts (NDFs; AllCells, LLC) were combined from three individual donors. NDFs were routinely tested for mycoplasma and confirmed to be negative using the MycoAlert mycoplasma detection kit (Lon | Full Paper | |
2022 | Oostindie, SC;Rinaldi, DA;Zom, GG;Wester, MJ;Paulet, D;Al-Tamimi, K;van der Meijden, E;Scheick, JR;Wilpshaar, T;de Jong, B;Hoff-van den Broek, M;Grattan, RM;Oosterhoff, JJ;Vignau, J;Verploegen, S;Boross, P;Beurskens, FJ;Lidke, DS;Schuurman, J;de Jong, RN; | Logic-gated antibody pairs that selectively act on cells co-expressing two antigens | Nature biotechnology | 35879362 | PBMCs derived from patients with CLL were commercially obtained from Discovery Life Sciences. Characteristics of patients with CLL are summarized in Supplementary Save | Full Paper |
2022 | Schuler, M;Cuppens, K;Ploenes, T;Vanbockrijck, M;Wiesweg, M;Darwiche, K;Schramm, A;Maes, B;Hegedus, B;Schildhaus, H;Hautzel, H;Theegarten, D;Baas, P;Hartemink, K;Du Pont, B;Aigner, C; | LBA37 A randomized, multicentric phase II study of preoperative nivolumab plus relatlimab or nivolumab in patients with resectable non-small cell lung cancer (NEOpredict-Lung) | Annals of Oncology | cology, AstraZeneca, Eisai, Takeda, ZytoVision, Zytomed Systems, Molecular Health; Financial Interests, Institutional, Research Grant: Novartis Oncology; Financial Interests, Personal, Full or part-time Employment: Targos Molecular Pathology Inc.. H. | Full Paper | |
2022 | Baraibar Argota, I;Garcia Rodriguez, A;Salvà Ballabrera, F;Ros Montana, F;Saoudi Gonzalez, N;Comas, R;Castillo, G;Sanchis, M;Hernando Cubero, J;García-Alvarez, A;Capdevila Castillon, J;Martí, M;Landolfi, S;Espin, E;Nuciforo, P;Vivancos, A;Tabernero, J;Elez Fernandez, M; | 332P Impact of the COVID-19 pandemic in the early-onset colorectal cancer (EOCRC) | Annals of Oncology | ersonal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. A. Vivancos: Financial Interests, Personal, Advisory Board: Roc | Full Paper | |
2022 | Lau, D;Khare, S;Stein, MM;Jain, P;Gao, Y;BenTaieb, A;Rand, TA;Salahudeen, AA;Khan, AA; | Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer | Nature communications | 35831288 | Samples for single-cell multi-omic sequencing were previously frozen dissociated tumor cells (DTCs) (Discovery Life Sciences, Huntsville, AL). DTCs were thawed and Save;xpression analyses were performed on log10-transformed counts. Single-cell mul | Full Paper |
2022 | Leung, DH;Devaraj, S;Goodrich, NP;Chen, X;Rajapakshe, D;Ye, W;Andreev, V;Minard, CG;Guffey, D;Molleston, JP;Bass, LM;Karpen, SJ;Kamath, BM;Wang, KS;Sundaram, SS;Rosenthal, P;McKiernan, P;Loomes, KM;Jensen, MK;Horslen, S;Bezerra, JA;Magee, JC;Merion, RM;Sokol, RJ;Shneider, BL;For ChiLDReN, ; | Serum Biomarkers Correlated with Liver Stiffness Assessed in a Multi-center Study of Pediatric Cholestatic Liver Disease | Hepatology (Baltimore, Md.) | 36069569 | similar distributions of age- and sex-matched children with normal liver biochemistries and without known liver disease served as case controls (Discovery Life Sciences, Save | Full Paper |
2022 | Yap, TA;Gainor, JF;Callahan, MK;Falchook, GS;Pachynski, RK;LoRusso, P;Kummar, S;Gibney, GT;Burris, HA;Tykodi, SS;Rahma, OE;Seiwert, TY;Papadopoulos, KP;Blum Murphy, M;Park, H;Hanson, A;Hashambhoy-Ramsay, Y;McGrath, L;Hooper, E;Xiao, X;Cohen, H;Fan, M;Felitsky, D;Hart, C;McComb, R;Brown, K;Sepahi, A;Jimenez, J;Zhang, W;Baeck, J;Laken, H;Murray, R;Trehu, E;Harvey, CJ; | First-in-Human Phase I/II ICONIC Trial of the ICOS Agonist Vopratelimab Alone and with Nivolumab: ICOS-High CD4 T-Cell Populations and Predictors of Response | Clinical cancer research : an official journal of the American Association for Cancer Research | 35511938 | Full Paper | |
2022 | Shalev, TJ;Gamal El-Dien, O;Yuen, MMS;Shengqiang, S;Jackman, SD;Warren, RL;Coombe, L;van der Merwe, L;Stewart, A;Boston, LB;Plott, C;Jenkins, J;He, G;Yan, J;Yan, M;Guo, J;Breinholt, JW;Neves, LG;Grimwood, J;Rieseberg, LH;Schmutz, J;Birol, I;Kirst, M;Yanchuk, AD;Ritland, C;Russell, JH;Bohlmann, J; | The western redcedar genome reveals low genetic diversity in a self-compatible conifer | Genome research | 36109148 | University of British Columbia; tal.shalev@msl.ubc.ca.++University of British Columbia.++University of British Columbia.++Department of Energy Joint Genome Institute.++Canada's Michael Smith Genome Sciences Centre.++Canada's Michael Smith Genome Scie | Full Paper |
2022 | Cramer, DAT;Franc, V;Caval, T;Heck, AJR; | Charting the Proteoform Landscape of Serum Proteins in Individual Donors by High-Resolution Native Mass Spectrometry | Analytical chemistry | 36074704 | ditions. Experimental Procedures Individual serum samples from six healthy donors were provided by Sanquin Research (Amsterdam, The Netherlands). Serum samples of diseased donors were purchased from Discovery Life Sciences (Columbus, OH, USA). Furth | Full Paper |
2022 | Pawley, DC;Dikici, E;Deo, SK;Raccamarich, P;Fischl, MA;Alcaide, M;Daunert, S; | Rapid Point-of-Care Test Kit for Bacterial Vaginosis: Detection of Vaginolysin and Clue Cells Using Paper Strips and a Smartphone | Analytical chemistry | 35943181 | Purchased patient samples from Discovery Life Sciences confirmed positive for BV were also tested. All patient samples tested showed elevated levels of VLY, indicating a Save | Full Paper |
2022 | Nuti, S;Zhang, Y;Zerrouki, N;Roach, C;Bänfer, G;Kumar, GL;Manna, E;Diezko, R;Kersch, K;Rüschoff, J;Jasani, B; | High interobserver and intraobserver reproducibility among pathologists assessing PD-L1 CPS across multiple indications | Histopathology | 35993150 | Diezko is an employee of Targos Molecular Pathology GmbH, which performed pharmaceutical industry-sponsored professional training for pathologists and clinical trial Save;Biomarkers and Diagnostics, Oncology, Global Medical and Scientific Affairs, | Full Paper |
2022 | Loibl, S;Huang, CS;Mano, MS;Mamounas, EP;Geyer, CE;Untch, M;Thery, JC;Schwaner, I;Limentani, S;Loman, N;Lübbe, K;Chang, JC;Hatschek, T;Tesarowski, D;Song, C;Lysbet de Haas, S;Boulet, T;Lambertini, C;Wolmark, N; | Adjuvant trastuzumab emtansine in HER2-positive breast cancer patients with HER2-negative residual invasive disease in KATHERINE | NPJ breast cancer | 36117201 | es obtained from the pretreatment primary tumor biopsy material (or residual tumor tissue from definitive surgery post-NAST). HER2 status of all samples was centrally assessed in the same laboratory (Targos Molecular Pathology, GmbH [Kassel, Germany] | Full Paper |
2022 | Seok, Y;Yin, Q;Li, R;Mauk, M;Bai, H;Bau, H; | Manually-operated, slider cassette for multiplexed molecular detection at the point of care | Sensors and Actuators B: Chemical | HCV genotype 1 plasma sample (CV900106678051314DD, 69,000,000 IU/mL) for clinical research was purchased from Discovery life science Inc. (Huntsville, AL, USA) | Full Paper | |
2022 | Singampalli, KL;Li, J;Lillehoj, PB; | Rapid magneto-enzyme-linked immunosorbent assay for ultrasensitive protein detection | Analytica chimica acta | 36038242 | falciparum infection obtained in Uganda under IRB/EC approval for general research use were purchased from Discovery Life Sciences. All human samples were de- Save; Blood samples from donors with P. falciparum infection obtained in Uganda under IRB/ | Full Paper |
2022 | Koehler, VF;Adam, P;Fuss, CT;Jiang, L;Berg, E;Frank-Raue, K;Raue, F;Hoster, E;Knösel, T;Schildhaus, HU;Negele, T;Siebolts, U;Lorenz, K;Allelein, S;Schott, M;Spitzweg, C;Kroiss, M;German Study Group for Rare Malignant Tumors of the Thyroid and Parathyroid Glands, ; | Treatment of RET-Positive Advanced Medullary Thyroid Cancer with Multi-Tyrosine Kinase Inhibitors-A Retrospective Multi-Center Registry Analysis | Cancers | 35884466 | US is an employee of Targos Molecular Pathology, Inc., and received honoraria from or served as an advisory board member at Roche, Novartis Oncology, MSD, BMS, Save Cached | Full Paper |
2022 | Molina, SA;Davies, SJ;Sethi, D;Oh, S;Durand, N;Scott, M;Davies, LC;Wormuth, K;Clarke, D; | Particulates are everywhere, but are they harmful in cell and gene therapies? | Cytotherapy | 36175323 | International Society for Cell & Gene Therapy Process Development, Manufacturing, and Analytics Committee, Vancouver, Canada. Electronic address: sam.molina@gmail.com.++International Society for Cell & Gene Therapy Process Development, Manufacturing, | Full Paper |
2022 | Ren, Z;Chen, S;Qin, X;Li, F;Guo, L; | Study of the roles of cytochrome P450 (CYPs) in the metabolism and cytotoxicity of perhexiline | Archives of toxicology | 36083301 | Perhexiline is a prophylactic antianginal agent developed in the 1970s. Although, therapeutically, it remained a success, the concerns of its severe adverse effects Save;- Primary human hepatocytes (pooled from 10 donors) were obtained from Disc | Full Paper |
2022 | Cheng, LY;Dai, P;Wu, LR;Patel, AA;Zhang, DY; | Direct capture and sequencing reveal ultra-short single-stranded DNA in biofluids | iScience | 36147958 | N = 6 plasma samples from diseased individuals (3 from Type 1 Diabetes (T1D) patients and 3 from Alzheimer’s disease (AD) patients) were acquired from BioIVT’s biorepository and shipped frozen. Animal total blood samples were purchased from Discovery | Full Paper |
2022 | Kemper, K;Gielen, E;Boross, P;Houtkamp, M;Plantinga, T;de Poot, S;Burm, S;Janmaat, M;Koopman, L;van den Brink, E;Rademaker, R;Verzijl, D;Engelberts, P;Satijn, D;Sasser, A;Breij, E; | Mechanistic and pharmacodynamic studies of DuoBody-CD3x5T4 in preclinical tumor models | Life Science Alliance | Dissociated tumor cells (DTCs) were procured from commercial vendors (Prisma Health/KIYATEC Biorepository or Discovery Life Sciences) who maintain strict ethical compliance, including fully | Full Paper | |
2022 | Rüschoff, J;Friedrich, M;Nagelmeier, I;Kirchner, M;Andresen, LM;Salomon, K;Portier, B;Sredni, ST;Schildhaus, HU;Jasani, B;Grzelinski, M;Viale, G; | Comparison of HercepTest™ mAb pharmDx (Dako Omnis, GE001) with Ventana PATHWAY anti-HER-2/neu (4B5) in breast cancer: correlation with HER2 amplification and HER2 low status | Virchows Archiv : an international journal of pathology | 35970977 | in HER2 evaluation, having served over the past 20 years as readers in most of the trastuzumab, pertuzumab, and T-DM1 approval BC studies by Targos GmbH (Kassel, Save;Targos - A Discovery Life Sciences Company, Germaniastraße 7, 34119, Kassel, Germ | Full Paper |
2022 | Suwa, R;Kume, Y;Kawase, M;Chishiki, M;Ono, T;Norito, S;Sato, K;Okamoto, M;Kumaki, S;Nagai, Y;Hosoya, M;Takeda, M;Nishimura, H;Hashimoto, K;Shirato, K; | Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan | Pathogens (Basel, Switzerland) | 35889999 | To confirm amplification specificity, we used pharyngeal, nasopharyngeal, and nasal swabs from Discovery Life Sciences (Los Osos, CA, USA) that were obtained for the Save Cached | Full Paper |
2022 | Rodrigues, D;van Kampen, R;van Bodegraven, AA;Kleinjans, JCS;Jennen, DGJ;de Kok, TM; | Gene expression responses reflecting 5-FU-induced toxicity: Comparison between patient colon tissue and 3D human colon organoids | Toxicology letters | 36183961 | All patients provided written informed consent. Human colon tissue samples were obtained by Boehringer Ingelheim Pharmaceuticals Inc. (Ridgefield, Connecticut, USA) from Discovery Life Sciences (Huntsville, Alabama, USA; formerly, Conversant Biologic | Full Paper |
2022 | Cao, L;Ledeboer, A;Pan, Y;Lu, Y;Meyer, K; | Clinical enrollment assay to detect preexisting neutralizing antibodies to AAV6 with demonstrated transgene expression in gene therapy trials | Gene therapy | 35778500 | Individual human serum samples were purchased from BioIVT (Westbury, NY, USA), Discovery Life Sciences (Los Osos, CA, USA), and Golden West Biologicals (Temecula, CA, USA) | Full Paper |
2022 | Zhang, L;Wang, Y;Homan, KT;Gaudette, SM;McCluskey, AJ;Chan, Y;Murphy, J;Abdalla, M;Nelson, CM;Sun, VZ;Erickson, JE;Knight, HL;Clabbers, A;Sterman, AJS;Mitra, S; | Imaging the Alternatively Spliced D Domain of Tenascin C in a Preclinical Model of Inflammatory Bowel Disease | Molecular imaging and biology | 35906512 | Clinical IBD colon was evaluated using a biotinylated anti-TNC D antibody. Frozen human tissue was obtained from CHTN (National Cancer Institute, Bethesda, MD), Folio Biosciences (Columbus, OH), and UMASS Memorial Gastroenterology (Worcester, MA) | Full Paper |
2022 | Holmberg, JA;Henry, SM;Burnouf, T;Devine, D;Marschner, S;Boothby, TC;Burger, SR;Chou, ST;Custer, B;Blumberg, N;Siegel, DL;Spitalnik, SL; | National Blood Foundation 2021 Research and Development summit: Discovery, innovation, and challenges in advancing blood and biotherapies | Transfusion | 36169155 | consulting firm, and one of our clients is Discovery Life Sciences, an, apheresis services provider. My advice to DLS is the same as what I say in this manuscript. HemaCare Save | Full Paper |
2022 | Edmonds, NL;Flores, SE;Mahmutovic, A;Young, SJ;Mauldin, IS;Slingluff, CL; | CD103 and periplakin are potential biomarkers for response of metastatic melanoma to pembrolizumab | Melanoma research | 36169985 | QualTek provided a modified proportion score indicating the percent of PD-L1 Qualtek also reported an overall estimate of the presence/absence of tumor-infiltrating Save | Full Paper |
2022 | Kandi, S;Savaryn, JP;Ji, QC;Jenkins, GJ; | Use of In-Sample Calibration Curve approach for quantification of peptides with high resolution Mass Spectrometry | Rapid communications in mass spectrometry : RCM | 35940586 | Human Peripheral Blood Mononuclear Cells (hPBMCs) were purchased from Discovery Life Sciences (Newtown, PA, USA) | Full Paper |
2022 | Boillat, MA;Hauser, PC; | CO2-measuring dongle | HardwareX | 35873734 | 11 2.11 Mouser.com semiconductor U4 Sensirion SCD41-D-R1 1 45.66 45.66 Mouser.com CO2-sensor J1 GCT USB3505-30-A-KIT 1 2.35 2.35 Mouser.com Micro B USB 2.0 socket Qualtek 3025033-1 1 2.97 2.97 Mouser.com | Full Paper |
2022 | Schöniger, S;Rüschoff, J; | Mismatch Repair Deficiency and Microsatellite Instability | Encyclopedia | Targos—A Discovery Life Sciences Company, Germaniastrasse 7, 34119 Kassel, Germany | Full Paper | |
2022 | Li, W;Li, Y;Xu, J;Chen, C;Gu, Y;Lu, Z;Li, D;Li, J;Sorg, A;Roberts, C;Mahajan, S;Gallant, M;Taggart, D; | Multimodal Epigenetic Sequencing Analysis (MESA) of Cell-free DNA for Non-invasive Cancer Detection | Research Square | Cohort 1 subjects were recruited at clinical sites within the United States through the ELITE Study (NCT05181826) or were obtained through the following contract research organizations: BioIVT (Westbury, NY), BioOptions (Brea, CA), Discovery Life Sci | Full Paper | |
2022 | Koerner, L;Yang, T;Schmiel, M;Peifer, M;Buettner, R;Pasparakis, M; | NEMO- and RelA-dependent NF-?B Signaling Promotes Small Cell Lung Cancer | bioRxiv | Small cell lung cancer (SCLC) is an aggressive type of lung cancer driven by combined loss of the tumor suppressors RB1 and TP53. SCLC is highly metastatic and Save Cached | Full Paper | |
2022 | Hebda-Bauer, E;Hagenauer, M;Blandino, P;Meng, F;Chitre, A;Ozel, A;Arakawa, K;Flagel, S;Watson, S;Palmer, A;Li, J;Akil, H; | Transcriptional Profiling of the Hippocampus in an F2 Cross of a Genetic Rat Model of Internalizing vs. Externalizing Behavior and Addiction Liability | bioRxiv | HudsonAlpha Discovery provided a variety of metrics for evaluating sample RNA quality, including RNA concentration (RNAconc), RNA integrity number (RIN), the Save Cached | Full Paper | |
2022 | Chitre, A;Hebda-Bauer, E;Blandino, P;Bimschleger, H;Nguyen, K;Maras, P;Li, F;Ozel, A;Polysskaya, O;Cheng, R;Flagel, S;Watson, S;Li, J;Akil, H;Palmer, A; | Genome-Wide Association Study in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like Traits | bioRxiv | For these 20 rats, liver tissue was sent to HudsonAlpha Discovery (Discovery Life Sciences) for HMW genomic DNA extraction and long-range and deep sequencing. Save | Full Paper | |
2022 | Dejanovic, B;Wu, T;Tsai, M;Graykowski, D;Gandham, V;Rose, C;Bakalarski, C;Ngu, H;Wang, Y;Pandey, S;Rezzonico, M;Friedman, B;Edmonds, R;De Mazière, A;Rakosi-Schmidt, R;Singh, T;Klumperman, J;Foreman, O;Chang, M;Xie, L;Sheng, M;Hanson, J; | Complement C1q-dependent excitatory and inhibitory synapse elimination by astrocytes and microglia in Alzheimer’s disease mouse models | Nature Aging | CSF from patients with AD and healthy controls was obtained from Folio Biosciences and Precision Medicine with ethics committee approval and written informed consent | Full Paper | |
2022 | Cady, J;Greytak, E; | Whole-genome sequencing of degraded DNA for investigative genetic genealogy | Forensic Science International: Genetics Supplement Series | Samples were submitted to either HudsonAlpha Discovery or Arbor Biosciences for library preparation and sequencing. For each sample, a small amount of the prepared Save | Full Paper | |
2022 | Newman, L;Useckaite, Z;Rowland, A; | Addressing MISEV guidance using targeted LC?MS/MS: A method for the detection and quantification of extracellular vesicle?enriched and contaminant protein markers from blood | Journal of Extracellular Biology | Serum and plasma samples from patients with non-alcoholic fatty liver disease (NAFLD) were purchased from Discovery Life Sciences (Hunstville, AL, USA). Save | Full Paper | |
2022 | D'ANTONIO, L; | Esposizione a paracetamolo e acido sulfosalicilico: effetti biologici nel bivalve Mytilus spp. | Thesis | Francisca Piedade, Sofia Bio, Bruno Nunes. 2020 Effects of common pharmaceutical drugs | Full Paper | |
2022 | Wagner, RS; | Environmental Factors Affecting Rhizophydiales Sp. Infecting Planktothrix Spp. | Thesis | were then sent to HudsonAlpha Discovery Life Sciences (Hudson, AL USA) for sequencing. Sequencing and QC was done at HudsonAlpha Discovery Life Sciences using Save;- then sent to HudsonAlpha Discovery Life Sciences (Hudson, AL USA) for sequencin | Full Paper | |
2022 | Ferreira Garcia Rodrigues, D; | Gut feelings | maastricht university | Human healthy colon organoids were derived from a tissue biopsy collected from a healthy male donor (67 years old) and kindly provided by Boehringer Ingelheim Pharmaceuticals Inc. (Ridgefield, USA), who purchased tissue biopsies from Discovery Life S | Full Paper | |
2022 | Fang, G; | The use of perfusion MRI using ferumoxytol and small molecular weight gadolinium (Gd) agents to assess response to pembrolizumab in brain metastases and systemic lesions in NSCLC: A comparison of imaging modalities to address brain metastases, pseudoprogression and systemic lesion tumor flare (neuro-check pilot) | Thesis | Tissue samples will be sent to QualTek Molecular Laboratories for PD-L1 biomarker Brain Tissue samples will also be sent to QualTek Molecular Laboratories for PD-1 Save | Full Paper | |
2022 | Isakoff, SJ; | DF/HCC Protocol#: 17-328 TITLE: A Phase 1b Study of Safety and Immune Response to PVX-410 Vaccine Alone and in Combination with Pembrolizumab | webctportal.partners.org | Primary tumor slides will be evaluated for expression of PD-L1 using the Merck Preferred Vendor QualTek according to their standard procedures. A | Full Paper | |
2022 | Frankovi?, J; | Koncerti hrvatskih autora za violon?elo i orkestar | Thesis | Makedonskog teatra te je osniva? komornog ansambla za suvremenu glazbu „Sveta Sofia“. Bio je jedan od najplodnijih jugoslavenskih skladatelja srednje generacije te se okušao u svim stvarala?kim sferama | Full Paper | |
2022 | Yang, SYC; | The Molecular Determinants of Response to Immune Checkpoint Therapy in Solid Tumors | Thesis | on 4-5?m sections mounted on positively charged ProbeOn slides (QualTek, Goleta, CA). QualTek provided a modified proportion score (MPS) indicating the proportion of Save | Full Paper | |
2022 | Shen, Y;Guo, K;Ma, A;Huang, Z;Du, J;Chen, J;Lin, Q;Wei, C;Wang, Z;Zhang, F;Zhang, J;Lin, W;Feng, N;Ma, W; | Mitochondrial toxicity evaluation of traditional Chinese medicine injections with a dual in vitro approach | Frontiers in pharmacology | 36408232 | and Health Sciences, Wuyi University, Jiangmen, China 3 Antibody Engineering Laboratory, School of Life Science & Technology, China Pharmaceutical University, Nanjing, China Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States Review | Full Paper |
2022 | Tiu, C;Lopez, J; | Lisavanbulin. Microtubule destabilizer (tubulin polymerization inhibitor), Tumor checkpoint controller, Treatment of advanced glioblastoma multiforme | Drugs of the Future | Assessment of 108 clinical trial samples from NCT02490800, using a CE-marked immunohistochemistry Clinical Trial Assay (CTA; Targos Molecular Pathology GmbH, Save | Full Paper | |
2022 | Drapkin, R;Jung, E;Bradshaw, C;Demars, L;Mosher, R; | EP014/#408?Evaluation of NAPI2B expression in a well annotated longitudinal tissue series of ovarian serous carcinomas | E-Posters | IHC analyses were performed by QualTek Molecular Laboratories (Discovery Life Sciences). Editorial support for this poster was provided by BluPrint Oncology. Save | Full Paper | |
2022 | Korner, LK; | Cell death and inflammation in skin homeostasis and small cell lung cancer | Thesis | R.B. is an employee of Targos Molecular Pathology. The other authors declare no competing interests | Full Paper | |
2022 | Weng, H;Huang, F;Yu, Z;Chen, Z;Prince, E;Kang, Y;Zhou, K;Li, W;Hu, J;Fu, C;Aziz, T;Li, H;Li, J;Yang, Y;Han, L;Zhang, S;Ma, Y;Sun, M;Wu, H;Zhang, Z;Wunderlich, M;Robinson, S;Braas, D;Hoeve, JT;Zhang, B;Marcucci, G;Mulloy, JC;Zhou, K;Tao, HF;Deng, X;Horne, D;Wei, M;Huang, H;Chen, J; | The m6A reader IGF2BP2 regulates glutamine metabolism and represents a therapeutic target in acute myeloid leukemia | Cancer cell | 36306790 | Bone marrow (BM) cells were collected from 5- to 7-week-old wild-type, Mettl14fl/flCreERT, or Igf2bp2fl/fl mice, and BM progenitor (HSPC, i.e., Lin- or c-kit+) cells were enriched with the Mouse Lineage Cell Depletion Kit or the CD117 microbeads (Mil | Full Paper |
2022 | Wei, J;Montalvo-Ortiz, W;Yu, L;Krasco, A;Olson, K;Rizvi, S;Fiaschi, N;Coetzee, S;Wang, F;Ullman, E;Ahmed, HS;Herlihy, E;Lee, K;Havel, L;Potocky, T;Ebstein, S;Frleta, D;Khatri, A;Godin, S;Hamon, S;Brouwer-Visser, J;Gorenc, T;MacDonald, D;Hermann, A;Chaudhry, A;Sirulnik, A;Olson, W;Lin, J;Thurston, G;Lowy, I;Murphy, AJ;Smith, E;Jankovic, V;Sleeman, MA;Skokos, D; | CD22-targeted CD28 bispecific antibody enhances antitumor efficacy of odronextamab in refractory diffuse large B cell lymphoma models | Science translational medicine | 36350988 | For the NALM6-luc tumor experiments, 12-week-old female immunodeficient NSG mice (Jackson Laboratory, 005557) were engrafted with 4 × 10 6 human PBMCs (ReachBio), and Save | Full Paper |
2022 | Devoucoux, M;Fort, V;Khelifi, G;Xu, J;Alerasool, N;Galloy, M;Wong, N;Bourriquen, G;Fradet-Turcotte, A;Taipale, M;Hope, K;Hussein, SMI;Côté, J; | Oncogenic ZMYND11-MBTD1 fusion protein anchors the NuA4/TIP60 histone acetyltransferase complex to the coding region of active genes | Cell reports | 35705031 | Recombinant Mouse TPO PeproTech Cat# AF-315-14 Recombinant Mouse IL-3 R&D Systems Cat# 403-ML-050 Recombinant Mouse IL-6 R&D Systems Cat# 406-ML-025 ColonyGEL, Mouse Complete Medium ReachBio Cat# 1202 SFEM II Media STEMCELL Technologies Cat# 09655 | Full Paper |
2022 | Peterson, CW;Venkataraman, R;Reddy, SS;Pande, D;Enstrom, MR;Radtke, S;Humbert, O;Kiem, HP; | Intracellular RNase activity dampens zinc finger nuclease-mediated gene editing in hematopoietic stem and progenitor cells | Molecular therapy. Methods & clinical development | 34977270 | nces, San José, CA) into D-PBS, followed by total genomic DNA extraction and NGS. Colony-forming assays ZFN- or CRISPR/Cas9-treated NHP cells (1.2 × 103) were plated in 3.6 mL of ColonyGEL 1402 (ReachBio, Seattle, WA). For colony-forming assays util | Full Paper |
2022 | Liu, L;Li, H;Patterson, AM;Plett, PA;Sampson, CH;Mohammad, KS;Capitano, ML;Singh, P;Yao, C;Orschell, CM;Pelus, LM; | Upregulation of SIRT1 Contributes to dmPGE2-dependent Radioprotection of Hematopoietic Stem Cells | Stem cell reviews and reports | 35318613 | Colony forming cells were analyzed as described [20]. Total nucleated BM cells from non-irradiated mice were plated at 2 × 104 per dish in Mouse Complete Colony Gel media (Reach Bio, Seattle, WA). BM cells from irradiated C57BL/6 mice were plated at | Full Paper |
2022 | Canals Hernaez, D;Hughes, MR;Li, Y;Mainero Rocca, I;Dean, P;Brassard, J;Bell, EM;Samudio, I;Mes-Masson, AM;Narimatsu, Y;Clausen, H;Blixt, O;Roskelley, CD;McNagny, KM; | Targeting a Tumor-Specific Epitope on Podocalyxin Increases Survival in Human Tumor Preclinical Models | Frontiers in oncology | 35600398 | Linker stability was independently assessed by ReachBio Research Labs (RRL, www.reachbio.com) using an in vitro neutrophil differentiation killing assay, where extracellular Save;Emer Clarke at ReachBio Research Labs (www.reachbio.com) for providin | Full Paper |
2022 | Lefavor, R;Clarke, E; | The Effect of Various Stimulants on Cytokine Secretion Profiles in Freshly Isolated Peripheral Blood Mononuclear Cells (PBMC) | The Journal of Immunology | 1ReachBio Research Labs++1ReachBio Research Labs | Full Paper | |
2022 | Gu, Q;Palani, CD;Smith, A;Li, B;Amos-Abanyie, EK;Ogu, U;Lu, L;Pace, BS;Starlard-Davenport, A; | MicroRNA29B induces fetal hemoglobin via inhibition of the HBG repressor protein MYB in vitro and in humanized sickle cell mice | Frontiers in medicine | 36507536 | kville, MD, USA) was used alone or co-transfected with MIR29B. Erythroid differentiation of human CD34+ stem cells and microRNA29B co-electroporation with MYB DNA Human bone marrow CD34+ stem cells (ReachBio, Seattle, WA, USA) were cultured in a mod | Full Paper |
2022 | Balk, R;Esper, A;Martin, G;Miller, R;Lopansri, B;Burke, J;Levy, M;Opal, S;Rothman, R;D'Alessio, F;Sidhaye, V;Aggarwal, N;Greenberg, J;Yoder, M;Patel, G;Gilbert, E;Parada, J;Afshar, M;Kempker, J;van der Poll, T;Schultz, M;Scicluna, B;Klein Klouwenberg, P;Liebler, J;Blodget, E;Kumar, S;Navalkar, K;Yager, T;Sampson, D;Kirk, J;Cermelli, S;Davis, R;Brandon, R; | Validation of SeptiCyte RAPID to discriminate sepsis from non-infectious systemic inflammation | medRxiv | The authors thank the clinical study coordinators of the NEPTUNE study: Joyce D. Brown (Rush University), Liliacna Jara (University of Southern California), Leona Wells and Maya C. Whaley (Grady Memorial Hospital). The authors also thank the laborato | Full Paper | |
2022 | Vujovic, A;de Rooij, L;Chahi, A;Chen, H;Yee, B;Loganathan, S;Liu, L;Chan, D;Tajik, A;Tsao, E;Moreira, S;Joshi, P;Xu, J;Wong, N;Zandi, S;Aigner, S;Dick, J;Minden, M;Schramek, D;Yeo, G;Hope, K; | A two-step in vivo CRISPR screen unveils pervasive RNA binding protein dependencies for leukemic stem cells and identifies ELAVL1 as a therapeutic target | bioRxiv | Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio), supplemented with DMSO or 1.1uM DHTS. Colonies were scored on days 10-14. Human CB samples were | Full Paper | |
2022 | Keyvani Chahi, A; | CHARACTERIZING THE ROLE OF THE TRANSCRIPTION FACTOR PLAG1 IN HUMAN HEMATOPOIETIC STEM AND PROGENITOR CELLS | Thesis | Rapamycin 50nM Cedarlane/MedChemExpr ess HY-10219 ColonyGEL™ 1102 1X Reachbio 1102 Sterile, Blunt-End Needles, 16 Gauge with Luer Lock NA StemCell Technologies 28110 | Full Paper | |
2022 | Stuart, WR; | Inhibition of Embryonic Ectoderm Development as a Novel Mechanism for Fetal Hemoglobin Induction in Sickle Cell Disease | Thesis | Gating and analysis were performed on FlowJo. Single cells were gated using FSC H vs FSC A. CD235a vs CD71 double positive cells were used for HbF analysis through recommendation from ReachBio as these are the hemoglobin expressing cells within the p | Full Paper | |
2021 | Schneider, BP;Jiang, G;Ballinger, TJ;Shen, F;Chitambar, C;Nanda, R;Falkson, C;Lynce, FC;Gallagher, C;Isaacs, C;Blaya, M;Paplomata, E;Walling, R;Daily, K;Mahtani, R;Thompson, MA;Graham, R;Cooper, ME;Pavlick, DC;Albacker, LA;Gregg, J;Solzak, JP;Chen, YH;Bales, CL;Cantor, E;Hancock, BA;Kassem, N;Helft, P;O'Neil, B;Storniolo, AMV;Badve, S;Miller, KD;Radovich, M; | BRE12-158: A Postneoadjuvant, Randomized Phase II Trial of Personalized Therapy Versus Treatment of Physician's Choice for Patients With Residual Triple-Negative Breast Cancer | Journal of clinical oncology : official journal of the American Society of Clinical Oncology | 34910554 | Speakers' Bureau: Genomic Health, Targos Molecular Pathology, Agilent | Full Paper |
2021 | Vergote, I;González-Martín, A;Ray-Coquard, I;Harter, P;Colombo, N;Pujol, P;Lorusso, D;Mirza, MR;Brasiuniene, B;Madry, R;Brenton, JD;Ausems, MGEM;Büttner, R;Lambrechts, D;European experts’ consensus group, ; | European experts consensus: BRCA/homologous recombination deficiency testing in first-line ovarian cancer | Annals of oncology : official journal of the European Society for Medical Oncology | 34861371 | Testifying Advisor for MSD in GBA-assessment for Pembrolizumab, Advisor for Durvalumab. Co-Founder and CSO for Targos Molecular Pathology, Kassel/Germany until April 2021. DL: Consulting (2019-2021): AstraZeneca (2019-2021); BMS (2019); | Full Paper |
2021 | Voors-Pette, C;Aalders, W;Brill, M;Ferrari, D;Annis, A;Steidl, U;Aivado, M;Vukovic, V; | 1791P A phase I pharmacology study of the dual MDMX/MDM2 inhibitor, ALRN 6924, in healthy volunteers | Annals of Oncology | We thank the subjects who participated in these studies; Discovery Life Sciences in Newtown, PA; and Eric Smith for graphical assistance | Full Paper | |
2021 | Han, GR;Jang, H;Ki, H;Lee, H;Kim, MG; | Reagent Filming for Universal Point-of-Care Diagnostics | Small methods | 34928024 | A/B were purchased from Discovery Life Sciences (Los Osos, CA). T | Full Paper |
2021 | de Klerk, LK;Patel, AK;Derks, S;Pectasides, E;Augustin, J;Uduman, M;Raman, N;Akarca, FG;McCleary, NJ;Cleary, JM;Rubinson, DA;Clark, JW;Fitzpatrick, B;Brais, LK;Cavanaugh, ME;Rode, AJ;Jean, MG;Lizotte, PH;Nazzaro, MJ;Severgnini, M;Zheng, H;Fuchs, CS;Enzinger, PC;Bass, AJ; | Phase II study of pembrolizumab in refractory esophageal cancer with correlates of response and survival | Journal for immunotherapy of cancer | 34593617 | PD-L1 immunohistochemistry was performed on pretreatment FFPE tissue slides using a laboratory developed assay (QualTek Molecular Laboratories, Newtown, Pennsylvania, USA) with the anti-PD-L1 22C3 antibody (Merck & Co, Palo Alto, California, USA). | Full Paper |
2021 | Vowell, K;Conner, M;Perrin, F;Bojczuk, P;Hance, K;Roth, I;Donahue, C;Smothers, J;Waight, J; | 662 Dissecting the CD226 immune axis in the tumor microenvironment using CyTOF-based high-dimensional immunophenotyping | Journal for ImmunoTherapy of Cancer | Ethics Approval All samples were purchased from Discovery Life Sciences (DLS). DLS represents and warrants that it has ownership of all Products available for sale and has properly obtained, where required under HHS/OHRP 45 CFR 46.102 (d) (f), IRB | Full Paper | |
2021 | Binnewies, M;Pollack, JL;Rudolph, J;Dash, S;Abushawish, M;Lee, T;Jahchan, NS;Canaday, P;Lu, E;Norng, M;Mankikar, S;Liu, VM;Du, X;Chen, A;Mehta, R;Palmer, R;Juric, V;Liang, L;Baker, KP;Reyno, L;Krummel, MF;Streuli, M;Sriram, V; | Targeting TREM2 on tumor-associated macrophages enhances immunotherapy | Cell reports | 34686340 | Human tumor samples used for both flow cytometry and single-cell RNA sequencing were acquired from Discovery Life Sciences. 49 human tumor samples were used for flow cytometric analysis of immune composition and TREM2 surface levels. 10 human tumor | Full Paper |
2021 | Ma, J;Tan, X;Kwon, Y;Delgado, ER;Zarnegar, A;DeFrances, MC;Duncan, AW;Zarnegar, R; | A Novel Humanized Model of NASH and Its Treatment With META4, A Potent Agonist of MET | Cellular and molecular gastroenterology and hepatology | 34756982 | Background & Aims Nonalcoholic fatty liver disease is a frequent cause of hepatic dysfunction and is now a global epidemic. This ailment can progress to an advanced form called nonalcoholic steatohepatitis (NASH) and end-stage liver disease. Curren | Full Paper |
2021 | Bassez, A;Vos, H;Van Dyck, L;Floris, G;Arijs, I;Desmedt, C;Boeckx, B;Vanden Bempt, M;Nevelsteen, I;Lambein, K;Punie, K;Neven, P;Garg, AD;Wildiers, H;Qian, J;Smeets, A;Lambrechts, D; | A single-cell map of intratumoral changes during anti-PD1 treatment of patients with breast cancer | Nature medicine | 33958794 | combined with an intensity score, which represents the average intensity of positive tumor cells (0, none; 1, weak, 2, intermediate; 3, strong). PD-L1 (Merck 22C3 antibody) immunohistochemistry was performed by Qualtek Molecular Laboratories as per a | Full Paper |
2021 | Yuki, S;Nakamura, Y;Taniguchi, H;Denda, T;Nishina, T;Hamamoto, Y;Hara, H;Esaki, T;Kawakami, H;Takashima, A;Satoh, T;Sunakawa, Y;Masuishi, T;Shinozaki, E;Moriwaki, T;Miki, I;Shitara, K;Yoshino, T; | Expression of PD-L1 and PD-L2 in colorectal cancer (CRC): A post-hoc integrated analysis of SCRUM-Japan GI-SCREEN CRC. | Journal of Clinical Oncology | Patients (Pts) with MSI-H or BRAF V600E mutant tumors were prioritized to be included. PD-L1 (22C3) and PD-L2 expressions were centrally assessed using immunohistochemical assays at QualTek and NeoGenomics, respectively | Full Paper | |
2021 | Ho, CSL;Tüns, AI;Schildhaus, HU;Wiesweg, M;Grüner, BM;Hegedus, B;Schuler, M;Schramm, A;Oeck, S; | HER2 mediates clinical resistance to the KRASG12C inhibitor sotorasib, which is overcome by co-targeting SHP2 | European journal of cancer (Oxford, England : 1990) | 34715459 | HUS is an employee of Targos Molecular Pathology, Inc.; received research grants (outside this study) from Novartis Oncology; and received honoraria and advisory board services from MSD, BMS, Roche Pharma, Novartis Oncology, ZytoVision, Eisai and P | Full Paper |
2021 | Israeli, S;Golden, A;Atalig, M;Mekki, N;Rais, A;Storey, H;Barbouche, MR;Peck, R; | A Novel Point-of-Care Rapid Diagnostic Test for Screening Individuals for Antibody Deficiencies | Journal of clinical immunology | 34839430 | These thirty-three specimens were acquired from Discovery Life Sciences (Los Osos, CA, USA) with IgG concentration characterized by Siemens BN II System nephelometer (Siemens Healthcare GmbH, Erlangen, Germany). IgG levels in the chosen samples Sa | Full Paper |
2021 | Cheng, Y;Hall, TR;Xu, X;Yung, I;Souza, D;Zheng, J;Schiele, F;Hoffmann, M;Mbow, ML;Garnett, JP;Li, J; | Targeting uPA-uPAR interaction to improve intestinal epithelial barrier integrity in inflammatory bowel disease | EBioMedicine | 34933179 | anoids isolation and culture Isolation and culture of primary intestinal organoids was performed as previously described.2223 Briefly, human small intestinal tissues (obtained from Coversant Bio, now Discovery Life Sciences, https://www.dls.com/biosp | Full Paper |
2021 | Cheng, L;Creasy, T;Pilataxi, F;Greenlees, L;Vence, L;Sridhar, S;Streicher, K; | Effects of combination treatment with durvalumab plus tremelimumab on the tumor microenvironment in non-small-cell lung carcinoma | Cancer immunology, immunotherapy : CII | 34623465 | Frozen NSCLC tumor digests (untreated patients, three squamous cell carcinomas and one adenocarcinoma) were purchased from Discovery Life Sciences and cultured in 96-well plates in a medium containing low-dose interleukin 2 (IL-2) plus 20 µg/mL D a | Full Paper |
2021 | Bröske, AE;Korfi, K;Belousov, A;Wilson, S;Ooi, CH;Bolen, CR;Canamero, M;Alcaide, EG;James, I;Piccione, EC;Carlile, DJ;Dimier, N;Umaña, P;Bacac, M;Weisser, M;Dickinson, M; | Pharmacodynamics and molecular correlates of response to glofitamab in relapsed/refractory non-Hodgkin lymphoma | Blood advances | 34941996 | Biopsies were sent to the central pathology laboratory (Targos Molecular Pathology GmbH, Kassel, Germany) and fresh tissues were embedded on receipt. All biopsies were quality checked by a pathologist on hematoxylin and eosin (H&E) staining | Full Paper |
2021 | Howard, FM;Villamar, D;He, G;Pearson, AT;Nanda, R; | The emerging role of immune checkpoint inhibitors for the treatment of breast cancer | Expert opinion on investigational drugs | 34569400 | PD-L1 status was initially assessed with the QualTek PD-L1 assay and subsequently with the Dako 22C3 PD-L1 assay (with With re-analysis of PD-L1 status, seven patients who had tested positive with the QualTek assay were negative by the 22C3 assay | Full Paper |
2021 | Manos, J;Preiss, C;Venkat, N;Tamm, J;Reinhardt, P;Kwon, T;Wu, J;Winter, A;Jahn, T;Yanamandra, K;Titterton, K;Karran, E;Langlois, X; | UNCOVERING SPECIFICITY OF ENDOGENOUS TAU AGGREGATION IN A HUMAN IPSC-NEURON TAU SEEDING MODEL | iScience | (Braak stage V/VI) and non-AD (Braak stage 0/I) male and female donors used in preparing sarkosyl-insoluble tau seeds were generously provided by the Massachusetts Alzheimer's Disease Research Center or purchased from Folio (now Discovery Life Scie | Full Paper | |
2021 | Da Mesquita, S;Papadopoulos, Z;Dykstra, T;Brase, L;Farias, FG;Wall, M;Jiang, H;Kodira, CD;de Lima, KA;Herz, J;Louveau, A;Goldman, DH;Salvador, AF;Onengut-Gumuscu, S;Farber, E;Dabhi, N;Kennedy, T;Milam, MG;Baker, W;Smirnov, I;Rich, SS;Dominantly Inherited Alzheimer Network, ;Benitez, BA;Karch, CM;Perrin, RJ;Farlow, M;Chhatwal, JP;Holtzman, DM;Cruchaga, C;Harari, O;Kipnis, J; | Meningeal lymphatics affect microglia responses and anti-Aβ immunotherapy | Nature | 33911285 | FC-404-2001) cartridges. Processing of total RNA extracted from mouse meningeal LECs (including linear RNA amplification and cDNA library generation) and RNA-seq was performed by HudsonAlpha Genomic Services Laboratory. For bulk RNA-seq experiments w | Full Paper |
2021 | Matusz-Fisher, A;Tan, AR; | Combination of HER2-targeted agents with immune checkpoint inhibitors in the treatment of HER2-positive breast cancer | Expert opinion on biological therapy | 34806498 | PD-L1-positivity was defined as staining in ≥ 1% of tumor cells or any staining in the stroma using the QualTek immunohistochemistry (IHC) assay. Of note, the assay was changed to the Dako IHC 22C3 pharmDx Q 2 Solutions assay mid-trial due to the p | Full Paper |
2021 | Luk, KC;Gersch, J;Harris, BJ;Holzmayer, V;Mbanya, D;Sauleda, S;Rodgers, MA;Cloherty, G; | More DNA and RNA of HBV SP1 splice variants are detected in genotypes B and C at low viral replication | Scientific reports | 34903774 | genotyping through the Abbott Global Surveillance Program38. All other plasma specimens sourced from France, Germany, Italy, Spain and Vietnam with known viral loads and genotypes were purchased from Discovery Life Sciences (Huntsville, AL). HBV DNA | Full Paper |
2021 | Connolly, P;Stapleton, S;Mosoyan, G;Fligelman, I;Tonar, YC;Fleming, F;Donovan, MJ; | Analytical validation of a multi-biomarker algorithmic test for prediction of progressive kidney function decline in patients with early-stage kidney disease | Clinical proteomics | 34789168 | one specimens collected from patients receiving treatment with biologic therapeutic TNF-α inhibitors used to treat rheumatoid and psoriatic arthritis and other auto-immune diseases were sourced from Discovery Life Sciences (Huntsville, AL). Specimens | Full Paper |
2021 | Jöhrens, K;Rüschoff, J; | The Challenge to the Pathologist of PD-L1 Expression in Tumor Cells of Non-Small-Cell Lung Cancer-An Overview | Current oncology (Toronto, Ont.) | 34940076 | Institute of Pathology, Carl Gustav Carus University Hospital Dresden, 01307 Dresden, Germany.++Targos Molecular Pathology GmbH, 34119 Kassel, Germany | Full Paper |
2021 | Hou, X;Wang, G;Fan, W;Chen, X;Mo, C;Wang, Y;Gong, W;Wen, X;Chen, H;He, D;Mo, L;Jiang, S;Ou, M;Guo, H;Liu, H; | T-cell receptor repertoires as potential diagnostic markers for patients with COVID-19 | International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases | 34688948 | ad recovered from COVID-19. In the COVID-19-BWNW group, whole blood samples were collected at Bloodworks Northwest (Seattle, WA, USA). In the COVID-19-DLS group, whole blood samples were collected at Discovery Life Sciences (Huntsville, AL, USA). In | Full Paper |
2021 | Brosius, C;Caron, K;Sosnoff, C;Blount, B;Wang, L; | Rapid Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method to Measure Cannabinoids in Bronchoalveolar-Lavage Fluid of Patients with e-Cigarette, or Vaping, Product Use-Associated Lung Injury | ACS Omega | BAL pools were created using anonymous BAL samples acquired commercially from Discovery Life Sciences (Huntsville, AL). They were shipped frozen on dry ice and then stored in −70 C freezers until analyzed. Ten individual BAL fluids were screened fo | Full Paper | |
2021 | Sun, Q;Pastor, L;Du, J;Powell, MJ;Zhang, A;Bodmer, W;Wu, J;Zheng, S;Sha, MY; | A novel xenonucleic acid-mediated molecular clamping technology for early colorectal cancer screening | PloS one | 34610014 | give an average DNA fragment length of about 150 bp, which mimics the size of cfDNA fragments, justifying their use as cfDNA references. Except for the 10 CRC whole blood samples were purchased from Discovery Life Sciences (Huntsville, AL, US), All | Full Paper |
2021 | Pound, H;Gann, E;Wilhelm, S; | A comparative study of metatranscriptomic assessment methods to characterize Microcystis blooms | Limnology and Oceanography: Methods | Extracted RNA was processed using a Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus and then 50-million 100-bp paired-end reads were generated on the Illumina NovaSeq platform at Hudson Alpha Discovery Life Sciences (Huntsville, Sa | Full Paper | |
2021 | Miao, LY;Kim, HJ;Whitlatch, K;Jaiswal, D;Navarro, A;Egan, R;Olivo, PD; | A rapid homogenous bioassay for detection of thyroid-stimulating antibodies based on a luminescent cyclic AMP biosensor | Journal of immunological methods | 34871593 | TSI-positive serum samples were a kind gift of Dr. George Kahaly. Anti-thyroid peroxidase (TPO) antibody-positive human serum samples and nonthyroidal autoimmune disease state human serum samples were obtained from Discovery Life Science (Los Osos, C | Full Paper |
2021 | Nuovo, G;Suster, D;Tili, E;Awad, H;Magro, C; | A Standardization Protocol for the In Situ Detection of SARS-CoV2 RNA and Proteins | Applied Immunohistochemistry & Molecular Morphology | vid MD‡; Tili, Esmerina PhD§; Awad, Hamdy MD§; Magro, Cynthia MD∥ Author Information __ *Ohio State University Comprehensive Cancer Center §Ohio State University Medical Center, Columbus †Discovery Life Sciences, Powell, OH ‡Department of Patholo | Full Paper | |
2021 | Nguyen, M;Gary, J;Sonoda, J;Low, M;Ring, C;Bishop, J; | Feasibility of Lateral Flow Assays for Urinary Biomarkers to Nicotine Delivery Products | OSF PREPRINTS2021 | A prospective collection of 212 individual urine specimens from users and non-nicotine users was completed between July 2020 and April 2021 by Discovery Life Sciences (Los Osos, CA). All enrollees selfidentified as non-users (112 samples) or single | Full Paper | |
2021 | Zhang, L;Pohl, C;Homan, K;Sun, V;Gaudette, S;Nelson, C;Erickson, J;Knight, H;Bose, S;Lynch, G;McRae, B;Sterman, A;Mitra, S; | Imaging the Alternatively Spliced D Domain of Tenascin C in Preclinical Models of Inflammatory Bowel Disease | Research Square | Clinical IBD and diseased cynomolgus macaque colon were evaluated using a biotinylated anti-TNC D antibody. Frozen human tissue was obtained from CHTN (National Cancer Institute, Bethesda, MD), Folio Biosciences (Columbus, OH), and UMASS Memorial Gas | Full Paper | |
2021 | Kehl, F;Cretu, V;Willis, P; | Open-source lab hardware: Driver and temperature controller for high compliance voltage, fiber-coupled butterfly lasers | HardwareX | We recommend applying 12 VDC at the voltage input (VIN), eg, by connecting a wall adapter with sufficient power such as the Qualtek QFWB-65-12-US01, which can provide up to 5 A at 12 VDC. The input voltage is directly fed into a voltage regulator ( | Full Paper | |
2021 | Lockhart, JW;Jacobs, AZ; | Scientific Argument with Supervised Learning | NeurIPS 2021 AI for Science Workshop | ML for scientific discovery Life sciences often advance through creating and testing taxonomies or classifications of entities. Statistical methods to evaluate the theorized differences between groups offer the opportunity to validate such classifi | Full Paper | |
2021 | Hair, J;Robinson, MJ;Wilkinson, RW;Dovedi, SJ; | Deep phenotyping of surface stimulatory and inhibitory co-receptors on cancer-resident T and NK cells reveals cell subsets within the tumor-reactive CTL population that are uniquely defined by NKG2A expression | SLAS Discovery | Disaggregated tumor cells (DTC; Discovery Life Sciences), which are a single-cell suspension of enzymatically-dissociated tumor tissue, from NSCLC (n=13), RC (n=10) and melanoma (n=10) patients were used. Detailed per-patient disease classification | Full Paper | |
2021 | Edmund, M; | RISING STARS | Quality Progress | Quality associate, lead auditor, Discovery Life Sciences (DLS), Powell, OH | Full Paper | |
2021 | Valle, J;Kelley, R;Nervi, B;Oh, D;Zhu, A; | Biliary tract cancer | The Lancet | In KEYNOTE-028 PD-L1 positivity was defined as membranous PD-L1 expression in at least 1% of tumour and associated inflammatory cells, or positive staining in stroma (immunohistochemistry assay, QualTek, Goleta, CA, USA), and all patients were PD-L1 | Full Paper | |
2021 | Tiu, C;Derby, S;Haris, N;Welsh, L;Stansfeld, A;Hundsberger, T;Roth, P;König, F;Eisner, J;Kleinschmidt, M;Anderson, S;Bachmann, F;Lane, H;Engelhardt, M;Kaindl, T;Litherland, K;Stan, A;Evans, T;Plummer, E;Lopez, J; | The potential utility of end-binding protein 1 (EB1) as response-predictive biomarker for lisavanbulin: A phase 2 study of lisavanbulin (BAL101553) in adult patients with recurrent glioblastoma. | Journal of Clinical Oncology | Gallen, Switzerland; Department of Neurology, University Hospital Zurich, Zurich, Switzerland; Targos Molecular Pathology GmbH, Kassel, Germany; GeneCentric Therapeutics, Inc., Research Triangle Park, NC; Basilea Pharmaceutica International Ltd., Bas | Full Paper | |
2021 | Skowronska, M;Tiu, C;Tzankov, A;König, F;Lewis, J;Vivanco, I;Kleinschmidt, M;Beebe, K;Anderson, S;Bachmann, F;Engelhardt, M;Lane, H;Kaindl, T;Stan, A;Plummer, E;Evans, T;Zlobec, I;Lopez, J; | Expression of end-binding protein 1 (EB1), a potential response-predictive biomarker for lisavanbulin, in glioblastoma and various other solid tumor types. | Journal of Clinical Oncology | for articles by this author. Show More Institute of Pathology, University of Bern, Bern, Switzerland; The Royal Marsden NHS Foundation Trust, Sutton, United Kingdom; Institute of Pathology, University Hospital Basel, Basel, Switzerland; Targos Molecu | Full Paper | |
2021 | Pratto, F;Brick, K;Cheng, G;Lam, KG;Cloutier, JM;Dahiya, D;Wellard, SR;Jordan, PW;Camerini-Otero, RD; | Meiotic recombination mirrors patterns of germline replication in mice and humans | Cell | 34260899 | Human testicular biopsy from cancer patient (Normal adjacent tissue) Folio Biosciences now Discovery Life Sciences biospecimen bank https://www.dls.com | Full Paper |
2021 | Scoazec, J;Bauchet, A;Adam, J;Rochaix, P;MacGrogan, G;Kim, J;Nuciforo, P;López-Rios, F;Frisman, D;Cesarone, G;Bernstein, S;Lee, J;Henry, C;Ozoux, M;Lefebvre, A; | 1313P Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) tumor expression in a phase I/II study of tusamitamab ravtansine (SAR408701) in patients (pts) with advanced non-small cell lung cancer (NSCLC) | Annals of Oncology | articles by this author. D. Frisman D. Frisman. Affiliations Discovery Life Sciences, Goleta, CA, USA. Search for articles by this author. G. Cesarone G. Cesarone. Affiliations Discovery Life Sciences, Goleta, CA, USA. Search for articles | Full Paper | |
2021 | Salgado, R;Peg, V;Rüschoff, J;Vincent-Salomon, A;Castellano, I;Perner, S;Van de Vijver, K;Quinn, CM;Varga, Z; | Gene expression signatures for tailoring adjuvant chemotherapy of luminal breast cancer: the pathologists' perspective | Annals of oncology : official journal of the European Society for Medical Oncology | 34461263 | Department of Pathology, GZA-ZNA Hospitals, Antwerp, Belgium; Division of Research, Peter MacCallum Cancer Centre, Melbourne, Australia. Electronic address: roberto@salgado.be.++Universidad Autónoma de Barcelona, Barcelona, Spain; Department of Patho | Full Paper |
2021 | Keenan, TE;Guerriero, JL;Barroso-Sousa, R;Li, T;O'Meara, T;Giobbie-Hurder, A;Tayob, N;Hu, J;Severgnini, M;Agudo, J;Vaz-Luis, I;Anderson, L;Attaya, V;Park, J;Conway, J;He, MX;Reardon, B;Shannon, E;Wulf, G;Spring, LM;Jeselsohn, R;Krop, I;Lin, NU;Partridge, A;Winer, EP;Mittendorf, EA;Liu, D;Van Allen, EM;Tolaney, SM; | Molecular correlates of response to eribulin and pembrolizumab in hormone receptor-positive metastatic breast cancer | Nature communications | 34548479 | Tumors from 65/88 (74%) patients underwent PD-L1 testing assessed centrally by QualTek using the 22C3 antibody | Full Paper |
2021 | Brägelmann, J;Lorenz, C;Borchmann, S;Nishii, K;Wegner, J;Meder, L;Ostendorp, J;Ast, DF;Heimsoeth, A;Nakasuka, T;Hirabae, A;Okawa, S;Dammert, MA;Plenker, D;Klein, S;Lohneis, P;Gu, J;Godfrey, LK;Forster, J;Trajkovic-Arsic, M;Zillinger, T;Haarmann, M;Quaas, A;Lennartz, S;Schmiel, M;D'Rozario, J;Thomas, ES;Li, H;Schmitt, CA;George, J;Thomas, RK;von Karstedt, S;Hartmann, G;Büttner, R;Ullrich, RT;Siveke, JT;Ohashi, K;Schlee, M;Sos, ML; | MAPK-pathway inhibition mediates inflammatory reprogramming and sensitizes tumors to targeted activation of innate immunity sensor RIG-I | Nature communications | 34535668 | M.L.S. is a founder and shareholder of PearlRiver Bio (now part of Centessa Pharmaceuticals) and received consulting honoraria from PearlRiver Bio. M.L.S. receives research funding from PearlRiver Bio and Novartis. R.B. is an employee of Targos Molec | Full Paper |
2021 | Ny, L;Jespersen, H;Karlsson, J;Alsén, S;Filges, S;All-Eriksson, C;Andersson, B;Carneiro, A;Helgadottir, H;Levin, M;Ljuslinder, I;Olofsson Bagge, R;Sah, VR;Stierner, U;Ståhlberg, A;Ullenhag, G;Nilsson, LM;Nilsson, JA; | The PEMDAC phase 2 study of pembrolizumab and entinostat in patients with metastatic uveal melanoma | Nature communications | 34453044 | st was used to assess associations between immunohistochemical analyses (PD-L1 and TILs) and clinical benefit. Immunohistochemical analyses Tumor PD-L1 testing was performed at a central laboratory (QualTek Molecular Laboratories, Newtown, Pennsylva | Full Paper |
2021 | Cindy Yang, SY;Lien, SC;Wang, BX;Clouthier, DL;Hanna, Y;Cirlan, I;Zhu, K;Bruce, JP;El Ghamrasni, S;Iafolla, MAJ;Oliva, M;Hansen, AR;Spreafico, A;Bedard, PL;Lheureux, S;Razak, A;Speers, V;Berman, HK;Aleshin, A;Haibe-Kains, B;Brooks, DG;McGaha, TL;Butler, MO;Bratman, SV;Ohashi, PS;Siu, LL;Pugh, TJ; | Pan-cancer analysis of longitudinal metastatic tumors reveals genomic alterations and immune landscape dynamics associated with pembrolizumab sensitivity | Nature communications | 34446728 | ary Fig. 9A. PD-L1 immunohistochemistry Immunohistochemical (IHC) staining for PD-L1 using the mouse monoclonal anti-PD-L1 antibody (clone 22C3 at 2 μg/mL, Merck, Palo Alto, CA) was performed by Qualtek Molecular Laboratories (Newtown, PA, USA). The | Full Paper |
2021 | Fowler, TW;Mitchell, TL;Janda, CY;Xie, L;Tu, S;Chen, H;Zhang, H;Ye, J;Ouyang, B;Yuan, TZ;Lee, SJ;Newman, M;Tripuraneni, N;Rego, ES;Mutha, D;Dilip, A;Vuppalapaty, M;Baribault, H;Yeh, WC;Li, Y; | Development of selective bispecific Wnt mimetics for bone loss and repair | Nature communications | 34059688 | The following method was used for human and mouse femur bone (Fig. 1d, e). Fresh human cadaveric femur tissue was purchased from vendor (Folio Conversant). Femur heads were shipped on wet ice within 24 h of death and tissue used immediately upon rece | Full Paper |
2021 | Butler, D;Mozsary, C;Meydan, C;Foox, J;Rosiene, J;Shaiber, A;Danko, D;Afshinnekoo, E;MacKay, M;Sedlazeck, FJ;Ivanov, NA;Sierra, M;Pohle, D;Zietz, M;Gisladottir, U;Ramlall, V;Sholle, ET;Schenck, EJ;Westover, CD;Hassan, C;Ryon, K;Young, B;Bhattacharya, C;Ng, DL;Granados, AC;Santos, YA;Servellita, V;Federman, S;Ruggiero, P;Fungtammasan, A;Chin, CS;Pearson, NM;Langhorst, BW;Tanner, NA;Kim, Y;Reeves, JW;Hether, TD;Warren, SE;Bailey, M;Gawrys, J;Meleshko, D;Xu, D;Couto-Rodriguez, M;Nagy-Szakal, D;Barrows, J;Wells, H;O'Hara, NB;Rosenfeld, JA;Chen, Y;Steel, PAD;Shemesh, AJ;Xiang, J;Thierry-Mieg, J;Thierry-Mieg, D;Iftner, A;Bezdan, D;Sanchez, E;Campion, TR;Sipley, J;Cong, L;Craney, A;Velu, P;Melnick, AM;Shapira, S;Hajirasouliha, I;Borczuk, A;Iftner, T;Salvatore, M;Loda, M;Westblade, LF;Cushing, M;Wu, S;Levy, S;Chiu, C;Schwartz, RE;Tatonetti, N;Rennert, H;Imielinski, M;Mason, CE; | Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions | Nature communications | 33712587 | Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.++Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.++Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, U | Full Paper |
2021 | Li, J;Conrad, C;Mills, TW;Berg, NK;Kim, B;Ruan, W;Lee, JW;Zhang, X;Yuan, X;Eltzschig, HK; | PMN-derived netrin-1 attenuates cardiac ischemia-reperfusion injury via myeloid ADORA2B signaling | The Journal of experimental medicine | 33891683 | Materials and methods. MI patient and healthy donor samples. Serum/plasma samples of patients with MI or healthy donors were purchased from Discovery Life Sciences. Corresponding cTnI levels of patients with MI were documented in the patient sample d | Full Paper |
2021 | Dang, K;Castello, G;Clarke, SC;Li, Y;Balasubramani, A;Boudreau, A;Davison, L;Harris, KE;Pham, D;Sankaran, P;Ugamraj, HS;Deng, R;Kwek, S;Starzinski, A;Iyer, S;van Schooten, W;Schellenberger, U;Sun, W;Trinklein, ND;Buelow, R;Buelow, B;Fong, L;Dalvi, P; | Attenuating CD3 affinity in a PSMAxCD3 bispecific antibody enables killing of prostate tumor cells with reduced cytokine release | Journal for immunotherapy of cancer | 34088740 | Tumor cells were either isolated from fresh prostate tumor tissue procured from LF's lab at UCSF (San Francisco, California, USA) or received as frozen dissociated aliquots along with donor matched PBMCs from Discovery Life Sciences (Newtown, Pennsyl | Full Paper |
2021 | Nghiem, P;Bhatia, S;Lipson, EJ;Sharfman, WH;Kudchadkar, RR;Brohl, AS;Friedlander, PA;Daud, A;Kluger, HM;Reddy, SA;Boulmay, BC;Riker, A;Burgess, MA;Hanks, BA;Olencki, T;Kendra, K;Church, C;Akaike, T;Ramchurren, N;Shinohara, MM;Salim, B;Taube, JM;Jensen, E;Kalabis, M;Fling, SP;Homet Moreno, B;Sharon, E;Cheever, MA;Topalian, SL; | Three-year survival, correlates and salvage therapies in patients receiving first-line pembrolizumab for advanced Merkel cell carcinoma | Journal for immunotherapy of cancer | 33879601 | small T-antigen-specific serum antibodies21 or manifested large T-antigen expression in tumor biopsies via immunohistochemistry.22 PD-L1 staining (anti-PD-L1 clone 22C3, Merck & Co, Kenilworth, New Jersey, USA) was performed at QualTek Molecular | Full Paper |
2021 | Banchereau, R;Chitre, AS;Scherl, A;Wu, TD;Patil, NS;de Almeida, P;Kadel Iii, EE;Madireddi, S;Au-Yeung, A;Takahashi, C;Chen, YJ;Modrusan, Z;McBride, J;Nersesian, R;El-Gabry, EA;Robida, MD;Hung, JC;Kowanetz, M;Zou, W;McCleland, M;Caplazi, P;Eshgi, ST;Koeppen, H;Hegde, PS;Mellman, I;Mathews, WR;Powles, T;Mariathasan, S;Grogan, J;O'Gorman, WE; | Intratumoral CD103+ CD8+ T cells predict response to PD-L1 blockade | Journal for immunotherapy of cancer | 33827905 | containing regimen. Fresh tumor samples and matched adjacent non-cancerous tissues were procured from a commercial vendor (Discovery Life Sciences) as part of adult patients undergoing surgical resection. Online supplemental ;h locally advanced | Full Paper |
2021 | Badve, SS;Penault-Llorca, F;Reis-Filho, JS;Deurloo, R;Siziopikou, KP;D'Arrigo, C;Viale, G; | Determining PD-L1 Status in Patients with Triple-Negative Breast Cancer: Lessons Learned from IMpassion130 | Journal of the National Cancer Institute | 34286340 | CD has received consulting fees from Roche TD, Targos GmbH, Pfizer, Roche Pharma, LDPath Ltd, About Health Ltd, Uralensis INOV8 Ltd, and various NHS hospitals and has received nonfinan- cial support from UKNEQAS ICC&ISH and CADQAS cic | Full Paper |
2021 | Saura, C;Matito, J;Oliveira, M;Wildiers, H;Brufksy, AM;Waters, SH;Hurvitz, SA;Moy, B;Kim, SB;Gradishar, WJ;Queiroz, GS;Cronemberger, E;Wallweber, GJ;Bebchuk, J;Keyvanjah, K;Lalani, AS;Bryce, R;Vivancos, A;Eli, LD;Delaloge, S; | Biomarker Analysis of the Phase III NALA Study of Neratinib + Capecitabine versus Lapatinib + Capecitabine in Patients with Previously Treated Metastatic Breast Cancer | Clinical cancer research : an official journal of the American Association for Cancer Research | 34380637 | Yan (Puma Biotechnology). We thank Jeff Sperinde and Weidong Huang (Monogram Biosciences) for additional support for HERmark and p95 assay design and execution. We thank Sandra Bernard (Targos GMBH) for oversight of clinical sample management. We | Full Paper |
2021 | Pedersen, KS;Foster, NR;Overman, MJ;Boland, PM;Kim, SS;Arrambide, KA;Jaszewski, BL;Bekaii-Saab, T;Graham, RP;Welch, J;Wilson, RH;McWilliams, RR; | ZEBRA: A Multicenter Phase II Study of Pembrolizumab in Patients with Advanced Small-Bowel Adenocarcinoma | Clinical cancer research : an official journal of the American Association for Cancer Research | 33883178 | (clinicaltrials.gov). Biomarkers PD-L1 expression was centrally assessed (QualTek, Santa Barbara, CA) by immunohistochemical assays utilizing the Merck 22C3 antibody (Agilent/Dako). Samples required > 50 viable neoplastic cells. Modified Proportion S | Full Paper |
2021 | Larson, MH;Pan, W;Kim, HJ;Mauntz, RE;Stuart, SM;Pimentel, M;Zhou, Y;Knudsgaard, P;Demas, V;Aravanis, AM;Jamshidi, A; | A comprehensive characterization of the cell-free transcriptome reveals tissue- and subtype-specific biomarkers for cancer detection | Nature communications | 33883548 | We set out to validate the 23 cell-free mRNA biomarkers identified in our CCGA cohort in an orthogonal set of breast (n = 38) and lung (n = 18) cancer plasma samples obtained from a commercial vendor (Discovery Life Sciences) ;t to validate the | Full Paper |
2021 | Lorés-Motta, L;van Beek, AE;Willems, E;Zandstra, J;van Mierlo, G;Einhaus, A;Mary, JL;Stucki, C;Bakker, B;Hoyng, CB;Fauser, S;Clark, SJ;de Jonge, MI;Nogoceke, E;Koertvely, E;Jongerius, I;Kuijpers, TW;den Hollander, AI; | Common haplotypes at the CFH locus and low-frequency variants in CFHR2 and CFHR5 associate with systemic FHR concentrations and age-related macular degeneration | American journal of human genetics | 34260947 | associated with AMD (locus-wide conditioning).15 All the association analyses were performed with the “glm” R function.40 Immunocytochemistry of human retinas Human donor eyes were obtained from Discovery Life Sciences. Eyes were collected within 12 | Full Paper |
2021 | Egelston, C;Guo, W;Yost, S;Lee, JS;Rose, D;Avalos, C;Ye, J;Frankel, P;Schmolze, D;Waisman, J;Lee, P;Yuan, Y; | Pre-existing effector T-cell levels and augmented myeloid cell composition denote response to CDK4/6 inhibitor palbociclib and pembrolizumab in hormone receptor-positive metastatic breast cancer | Journal for immunotherapy of cancer | 33757987 | PD-L1 stain was performed using 22C3 antibody by Qualtek Molecular Laboratory | Full Paper |
2021 | Campbell, JR;McDonald, BR;Mesko, PB;Siemers, NO;Singh, PB;Selby, M;Sproul, TW;Korman, AJ;Vlach, LM;Houser, J;Sambanthamoorthy, S;Lu, K;Hatcher, SV;Lohre, J;Jain, R;Lan, RY; | Fc-optimized Anti-CCR8 Antibody Depletes Regulatory T Cells in Human Tumor Models | Cancer research | 33757978 | Study approval Human tumor, skin, spleen, patient blood, and healthy blood leukopack samples were collected through commercial providers (BioIVT, MT Group, Avaden, BioOptions, Discovery Life Sciences, AllCells) or the Cooperative Human Tissue Network | Full Paper |
2021 | Dieckmann, N;Schildhaus, HU;Bauer, S; | Tropomyosin receptor kinases in sarcomas - of joy and despair | Current opinion in oncology | 33989242 | Novartis, and Incyte; and received travel and accommodation funding from PharmaMar. H.U.S. is an employee of Targos Molecular pathology, Inc., received research funding from Novartis Oncology, has received honoria from MSD, BMS, Pfizer, Novartis Onco | Full Paper |
2021 | Yuan, Y;Lee, J;Yost, S;Frankel, P;Ruel, C;Egelston, C;Guo, W;Padam, S;Tang, A;Martinez, N;Schmolze, D;Presant, C;Ebrahimi, B;Yeon, C;Sedrak, M;Patel, N;Portnow, J;Lee, P;Mortimer, J; | Phase I/II trial of palbociclib, pembrolizumab and letrozole in patients with hormone receptor-positive metastatic breast cancer | European Journal of Cancer | 2.4. Tumour immune biomarkers Percentage of stromal TILs in tumour was evaluated using haematoxylin and eosin diagnostic sections per International TIL Work Group guideline [23]. PD-L1 was determined by immunohistochemistry (IHC) using the PD-L1 IHC | Full Paper | |
2021 | Liang, X;Wang, Y;Zhang, Y;Li, B;Radosevich, M; | Bacteriophage-host depth distribution patterns in soil are maintained after nutrient stimulation in vitro | The Science of the total environment | 33991924 | The extracted DNA samples were quantified using PicoGreen Assay Kit (Invitrogen, Carlsbad, CA) and were then sent to the HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA) for preparation of 16S rRNA gene amplicon library and high-throughp | Full Paper |
2021 | Quaas, A;Pamuk, A;Klein, S;Quantius, J;Rehkaemper, J;Barutcu, AG;Rueschoff, J;Zander, T;Gebauer, F;Hillmer, A;Buettner, R;Schroeder, W;Bruns, CJ;Löser, H;Schoemig-Markiefka, B;Alakus, H; | Sex-specific prognostic effect of CD66b-positive tumor-infiltrating neutrophils (TANs) in gastric and esophageal adenocarcinoma | Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association | 34009535 | Thomas Zander. Institute of Pathology, Nordhessen and Targos Molecular Pathology GmbH, Kassel, Germany. Josef Rueschoff. Authors: Alexander Quaas View author publications. You can also search for this author in PubMed Google Scholar | Full Paper |
2021 | Schoemig-Markiefka, B;Eschbach, J;Scheel, AH;Pamuk, A;Rueschoff, J;Zander, T;Buettner, R;Schroeder, W;Bruns, CJ;Loeser, H;Alakus, H;Quaas, A; | Optimized PD-L1 scoring of gastric cancer | Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association | 33954872 | Thomas Zander. Institute of Pathology Nordhessen, Kassel, Germany. Josef Rueschoff. Targos Molecular Pathology GmbH, Kassel, Germany. Josef Rueschoff & Reinhard Buettner. Authors: Birgid Schoemig-Markiefka View author publications | Full Paper |
2021 | Onogi, S;Lee, SH;Fruehauf, KR;Shea, KJ; | Abiotic Stimuli-Responsive Protein Affinity Reagent for IgG | Biomacromolecules | 34009976 | Transferrin (80 kDa) from human plasma was obtained from Calbiochem. Human serum was procured from Discovery Life Sciences, Inc. F(ab′) 2 (110 kDa) and Fc (50 kDa) fragments from human IgG were sourced from Jackson ImmunoResearch Laboratories, Inc | Full Paper |
2021 | Cao, XE;Kim, J;Mehta, S;Erickson, D; | Two-Color Duplex Platform for Point-of-Care Differential Detection of Malaria and Typhoid Fever | Analytical chemistry | 34469115 | samples in serum. 2.4. Validation with Human Clinical Samples. The clinical validation was conducted to evaluate the performance of the duplex platform with 25 human samples (Discovery Life Sciences). These included eight | Full Paper |
2021 | Narla, RK;Modi, H;Bauer, D;Abbasian, M;Leisten, J;Piccotti, JR;Kopytek, S;Eckelman, BP;Deveraux, Q;Timmer, J;Zhu, D;Wong, L;Escoubet, L;Raymon, HK;Hariharan, K; | Modulation of CD47-SIRPα innate immune checkpoint axis with Fc-function detuned anti-CD47 therapeutic antibody | Cancer immunology, immunotherapy : CII | 34247273 | lenalidomide [30]. Cell samples from AML patients were obtained from Conversant Bio (Huntsville) and are described in Supplemental Table 1. CC-90002 binding on cells and blockade of SIRPα to CD47 positive cells. Evaluation of | Full Paper |
2021 | Vos, H;Lambein, K;Richard, F;Mariën, B;Nevelsteen, I;Punie, K;Wildiers, H;Berben, L;Laenen, A;Floris, G;Desmedt, C;Smeets, A; | Comparison of the tumor immune microenvironment of primary hormone receptor-negative HER2-positive and triple negative breast cancer | NPJ breast cancer | 34556657 | PD-L1 (22C3 antibody, 2 μg/mL, Dako-Agilent) immunohistochemistry was performed by Qualtek Molecular Laboratories (Newtown PA, USA) as per agreement with Merck. QualTek provided a modified proportion score (MPS | Full Paper |
2021 | Pierson, SK;Shenoy, S;Oromendia, AB;Gorzewski, AM;Langan Pai, RA;Nabel, CS;Ruth, JR;Parente, SAT;Arenas, DJ;Guilfoyle, M;Reddy, M;Weinblatt, M;Shadick, N;Bower, M;Pria, AD;Masaki, Y;Katz, L;Mezey, J;Beineke, P;Lee, D;Tendler, C;Kambayashi, T;Fosså, A;van Rhee, F;Fajgenbaum, DC; | Discovery and validation of a novel subgroup and therapeutic target in idiopathic multicentric Castleman disease | Blood advances | 34438448 | this study. We wish to thank Daniel Martinez and the other members of the CHOP Pathology Core as well as Qualtek for their immunohistochemical analyses. We wish to thank Kevin Silk, Giovanni Insana, and James A. Lebovitz for their contributions to th | Full Paper |
2021 | Wang, A;Song, Z;Zheng, G;Nicolazzi, C;Fromm, JR;Shehu, E;Srinivasan, S;Chen, X;Zhu, C;Blondel, MC;Adrian, FJ; | Evaluation of Preclinical Activity of Isatuximab in Patients with Acute Lymphoblastic Leukemia | Molecular cancer therapeutics | 34376579 | centrifugation in line with the manufacturer's instructions. Patient ALL PBMCs were obtained from Conversant Biologics, Inc. Antibodies and reagents Isatuximab was generated in-house. Human immunoglobulin (Ig) G1, IgG4, ATRA | Full Paper |
2021 | Reardon, DA;Kim, TM;Frenel, JS;Simonelli, M;Lopez, J;Subramaniam, DS;Siu, LL;Wang, H;Krishnan, S;Stein, K;Massard, C; | Treatment with pembrolizumab in programmed death ligand 1-positive recurrent glioblastoma: Results from the multicohort phase 1 KEYNOTE-028 trial | Cancer | 33496357 | QualTek Molecular Laboratories evaluated archived or newly obtained formalin-fixed, paraffin-embedded tumor samples using the 22C3 PD-L1 antibody clone (Merck & Co., Inc., Kenilworth, NJ, USA) and a laboratory- developed prototype immunohistochemical | Full Paper |
2021 | Preillon, J;Cuende, J;Rabolli, V;Garnero, L;Mercier, M;Wald, N;Pappalardo, A;Denies, S;Jamart, D;Michaux, AC;Pirson, R;Pitard, V;Bagot, M;Prasad, S;Houthuys, E;Brouwer, M;Marillier, R;Lambolez, F;Marchante, JR;Nyawouame, F;Carter, MJ;Baron-Bodo, V;Marie-Cardine, A;Cragg, M;Déchanet-Merville, J;Driessens, G;Hoofd, C; | Restoration of T-cell Effector Function, Depletion of Tregs, and Direct Killing of Tumor Cells: The Multiple Mechanisms of Action of a-TIGIT Antagonist Antibodies | Molecular cancer therapeutics | 33277440 | Cryopreserved isolated PBMCs from healthy volunteers and patients with cancer were provided by ImmunXperts and ConversantBio, respectively. PBMCs were thawed, resuspended in RPMI medium supplemented with 10% FBS + 50 U/mL penicillin/streptomycin (Wes | Full Paper |
2021 | MacFarlane, AW;Yeung, HM;Alpaugh, RK;Dulaimi, E;Engstrom, PF;Dasari, A;Campbell, KS;Vijayvergia, N; | Impacts of pembrolizumab therapy on immune phenotype in patients with high-grade neuroendocrine neoplasms | Cancer immunology, immunotherapy : CII | 33398390 | PD-L1 immunohistochemistry (IHC) assay was performed at Qualtek Research Laboratories on formalin-fixed, paraffin-embedded tissue sections using anti-PD-L1 monoclonal antibody clone 22C3 (Merck Research Laboratories) | Full Paper |
2021 | Holder, C;Adams, A;Allison, C;Cote, O;Lippens, R;Blount, BC;Wang, L; | A Novel UHPLC-MS/MS Method for Measuring 8-iso-Prostaglandin F2α in Bronchoalveolar Lavage Fluid | Frontiers in chemistry | 34476231 | Non-EVALI BAL fluids used in this study were acquired from Discovery Life Sciences (Huntsville, AL, United States) | Full Paper |
2021 | Rodrigues, D;de Souza, T;Coyle, L;Di Piazza, M;Herpers, B;Ferreira, S;Zhang, M;Vappiani, J;Sévin, DC;Gabor, A;Lynch, A;Chung, SW;Saez-Rodriguez, J;Jennen, DGJ;Kleinjans, JCS;de Kok, TM; | New insights into the mechanisms underlying 5-fluorouracil-induced intestinal toxicity based on transcriptomic and metabolomic responses in human intestinal organoids | Archives of toxicology | 34151400 | 5-Fluorouracil (5-FU) is a widely used chemotherapeutical that induces acute toxicity in the small and large intestine of patients. Symptoms can be severe. ;- 5-Fluorouracil (5-FU) is a widely used chemotherapeutical that induces acute toxicity in | Full Paper |
2021 | Comunale, BA;Engineer, L;Jiang, Y;Andrews, JC;Liu, Q;Ji, L;Yurkovich, JT;Comunale, RA;Xie, Q; | Poliovirus Vaccination Induces a Humoral Immune Response That Cross Reacts With SARS-CoV-2 | Frontiers in medicine | 34414206 | ccination protocols, set by the Centers for Disease Control and Prevention (CDC). IRB approval for the collection of adult sera was obtained from BioMed IRB, and pediatric samples were purchased from Discovery Life Sciences (Huntsville, AL) in accord | Full Paper |
2021 | Haynes, WA;Kamath, K;Waitz, R;Daugherty, PS;Shon, JC; | Protein-Based Immunome Wide Association Studies (PIWAS) for the Discovery of Significant Disease-Associated Antigens | Frontiers in immunology | 33986742 | years, with a range of 22-72. Anti-Smith Cohort Samples from 34 subjects that tested positive for Anti-SM RNP (4) or Anti-Smith (30) antibodies by predicate ANA multiplex testing were obtained from Discovery Life Sciences. Subjects ranged in age fro | Full Paper |
2021 | Kerr, KM;Bibeau, F;Thunnissen, E;Botling, J;Ryška, A;Wolf, J;Öhrling, K;Burdon, P;Malapelle, U;Büttner, R; | The evolving landscape of biomarker testing for non-small cell lung cancer in Europe | Lung cancer (Amsterdam, Netherlands) | 33690091 | He is a co-owner and CSO of Targos Molecular Pathology, Inc., Germany | Full Paper |
2021 | Gentzel, RC;Toolan, D;Jinn, S;Schachter, JB;Ma, L;Kahle, PJ;Smith, SM;Marcus, JN; | Intracranial administration of alpha-synuclein fibrils in A30P-synuclein transgenic mice causes robust synucleinopathy and microglial induction | Neurobiology of aging | 34225000 | comparison. Neuropathologically confirmed human post-mortem PD brain tissue, acquired from Folio/Discovery Life Sciences (California, USA), or A30P HET brain tissues were serially extracted to obtain a sarkosyl insoluble fraction | Full Paper |
2021 | Malik-Chaudhry, HK;Prabhakar, K;Ugamraj, HS;Boudreau, AA;Buelow, B;Dang, K;Davison, LM;Harris, KE;Jorgensen, B;Ogana, H;Pham, D;Schellenberger, U;Van Schooten, W;Buelow, R;Iyer, S;Trinklein, ND;Rangaswamy, US; | TNB-486 induces potent tumor cell cytotoxicity coupled with low cytokine release in preclinical models of B-NHL | mAbs | 33818299 | ground value. The percent of specific lysis was calculated as described above. Ex vivo cytotoxicity assay The ability of TNB-486 to kill CD19+ tumor cells from CLL or DLBCL patient-derived samples (Discovery Life Sciences and IQ Biosciences) was det | Full Paper |
2021 | Yamada, M;Eguchi, A;Okuno, K;Sakaguchi, K;Yamaguchi, T; | Development of a highly sensitive chemiluminescent enzyme immunoassay for fragmented cytokeratin 18 using new antibodies | Scientific reports | 34521905 | All methods were carried out in accordance with relevant guidelines and regulations. Human samples. Serum from healthy individuals and NASH patients was purchased from Discovery Life Sciences (CA, USA) with approval and informed consent;defined below | Full Paper |
2021 | Awad, H;Tili, E;Nuovo, G;Kelani, H;Ramadan, ME;Williams, J;Binzel, K;Rajan, J;Mast, D;Efanov, AA;Rasul, KB;Moore, S;Basso, M;Mikhail, A;Eltobgy, M;Malbrue, RA;Bourekas, E;Oglesbee, M;Bergdall, V;Knopp, M;Michaille, JJ;El-Sayed, H; | Endovascular repair and open repair surgery of thoraco-abdominal aortic aneurysms cause drastically different types of spinal cord injury | Scientific reports | 33837260 | The remainder of the tissue was stored at − 80 °C for metabolome analyses. All sections were subsequently analyzed blindly. Immunohistochemistry (IHC). All sections were dealt with blindly using an automated Leica Bond Max platform at Phylogeny Inc. | Full Paper |
2021 | Mezache, L;Nuovo, G;Veeraraghavan, R; | A Multipronged Microscopy Approach Identifies Common Anti-Arrhythmic Strategy for Atrial Fibrillation and Myocardial Infarction | Microscopy and Microanalysis | 1 The Ohio State University, United States, 2 Discovery Life Sciences, United States Vascular leak and inflammation are associated with arrhythmic heart diseases such as atrial fibrillation (AF) and myocardial infarction (MI) | Full Paper | |
2021 | Ermann, J;Matmusaev, M;Haley, E;Braun, C;Jost, F;Mayer-Wrangowski, S;Hsiao, P;Ting, N;Li, L;Terenzio, D;Chime, J;Lukas, S;Patnaude, L;Panzenbeck, M;Csordas, D;Zheng, J;Mierz, D;Simpson, T;King, FJ;Klimowicz, A;Mbow, ML;Fine, JS;Miller, C;Fogal, S;Byrne, FR; | The potent and selective RIPK2 inhibitor BI 706039 improves intestinal inflammation in the TRUC mouse model of Inflammatory Bowel Disease | American journal of physiology. Gastrointestinal and liver physiology | 34494462 | Human IBD Intestinal biopsy Immuno-histo-chemical analysis 231 Tissue blocks from surgical resections for ulcerative colitis (n=6) and Crohn's Disease (n=5) were 232 acquired from Discovery Life Sciences. 4 µm sections were fluorescently stained usin | Full Paper |
2021 | Reisenauer, KN;Tao, Y;Das, P;Song, S;Svatek, H;Patel, SD;Mikhail, S;Ingros, A;Sheesley, P;Masi, M;Boari, A;Evidente, A;Kornienko, A;Romo, D;Taube, J; | Epithelial-mesenchymal transition sensitizes breast cancer cells to cell death via the fungus-derived sesterterpenoid ophiobolin A | Scientific reports | 34017048 | ke to acknowledge Dr. Michelle Nemec, Director of the Molecular Bioscience Center (Baylor University) for assistance with flow cytometry and Dr. Maria Boccia (Baylor University) and Dr. Gerard Nuovo (Discovery Life Sciences, Powell, OH, USA) for assi | Full Paper |
2021 | Caldwell, C;Rottman, JB;Paces, W;Bueche, E;Reitsma, S;Gibb, J;Adisetiyo, V;Haas, MS;Heath, H;Newman, W;Baum, J;Gianani, R;Kagey, MH; | Validation of a DKK1 RNAscope chromogenic in situ hybridization assay for gastric and gastroesophageal junction adenocarcinoma tumors | Scientific reports | 33972574 | chromogen kit (Biocare Medical). RNAscope. G/GEJ FFPE tumor resections were commercially acquired from Avaden Biosciences, Discovery Life Sciences or BioOptions following IRB compliance. RNAscope was performed ;emperature for 30 min, washed, and | Full Paper |
2021 | Wong, RL;Yu, EY; | Refining Immuno-Oncology Approaches in Metastatic Prostate Cancer: Transcending Current Limitations | Current treatment options in oncology | 33433743 | 028 phase 1b trial of pembrolizumab in PD-L1-positive advanced solid tumors included 23 patients with CRPC and PD-L1 expression ≥ 1% in tumor or stroma cells by a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek Molecular | Full Paper |
2021 | Meyer, L;Delgado-Cunningham, K;Lorig-Roach, N;Ford, J;DuBois, RM; | Human Astrovirus 1-8 Seroprevalence Evaluation in a United States Adult Population | Viruses | 34070419 | 2.7. Human Plasma Samples. Human plasma samples were obtained from Discovery Life Sciences Supplementary Materials. The following are available online at https://www.mdpi. com/article/10.3390/v13060979/s1, Table S1: Plasma Samples from Discovery Li | Full Paper |
2021 | Lopez-Anido, RN;Harrington, AM;Hamlin, HJ; | Coping with stress in a warming Gulf: the postlarval American lobster's cellular stress response under future warming scenarios | Cell stress & chaperones | 34115338 | The RNA samples from five replicate animals reared at each temperature were shipped to HudsonAlpha Discovery (formerly the Genomic Services Lab) at the HudsonAlpha Institute for Biotechnology (Huntsville, AL, USA) for library preparation and sequenci | Full Paper |
2021 | Magro, C;Crowson, A;Franks, L;Schaffer, P;Whelan, P;Nuovo, G; | The histologic and molecular correlates of COVID-19 vaccine-induced changes in the skin | Clinics in Dermatology | onald O. Perelman Department of Dermatology, New York University Grossman School of Medicine, New York, New York eDepartment of Pediatrics, Geffen School of Medicine at UCLA, Los Angeles, California fDiscovery Life Sciences, Powell, Ohio gThe Ohio St | Full Paper | |
2021 | Magro, C;Nuovo, G;Mulvey, J;Laurence, J;Harp, J;Crowson, A; | The skin as a critical window in unveiling the pathophysiologic principles of COVID-19 | Clinics in Dermatology | Joanna MD e Crowson A. Neil MD f aDepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York bThe Ohio State University Comprehensive Cancer Center, Columbus Ohio and Discovery Life Sciences, Powell, Ohio cDepartment | Full Paper | |
2021 | Xia, B;Blount, BC;Wang, L; | Sensitive Quantification of Nicotine in Bronchoalveolar Lavage Fluid by Acetone Precipitation Combined With Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry | ACS omega | 34124421 | We directly injected 1 μL of the residual supernatant onto the LC-MS/MS. BAL fluid pools were created for method validation experiments of the method from 12 anonymous BAL fluids purchased commercially from Discovery Life Sciences (Huntsville, AL) | Full Paper |
2021 | Cirillo, PDR;Margiotti, K;Fabiani, M;Barros-Filho, MC;Sparacino, D;Cima, A;Longo, SA;Cupellaro, M;Mesoraca, A;Giorlandino, C; | Multi-analytical test based on serum miRNAs and proteins quantification for ovarian cancer early detection | PloS one | 34352040 | All serum samples from ovarian cancer patients were obtained through MTA (Material Transfer Agreement) signed consent form from the following centers: Discovery Life Sciences (Huntsville, USA), BioIVT (London, UK), Victorian Cancer Bank (Melbourne, A | Full Paper |
2021 | Walsh, CS;Kamrava, M;Rogatko, A;Kim, S;Li, A;Cass, I;Karlan, B;Rimel, BJ; | Phase II trial of cisplatin, gemcitabine and pembrolizumab for platinum-resistant ovarian cancer | PloS one | 34081738 | that had membrane staining at low (1+) intensities or greater. An H-score was calculated ranging from 0–300, reflecting the percentage of cells staining at each of the following intensities: negative (1), low (1+), moderate (2+), high (3+). IHC assay | Full Paper |
2021 | Bernstein, DE;Trinh, HN;Schiff, ER;Smith, CI;Mospan, AR;Zink, RC;Fried, MW;Lok, AS; | Safety and Effectiveness of Tenofovir Alafenamide in Usual Clinical Practice Confirms Results of Clinical Trials: TARGET-HBV | Digestive diseases and sciences | 34059991 | ERS: Grant support: Eiger, Galmed, Zydus, Celgene, Beckman, Biokit, Bristol Myers Squibb, Conatus, Celgene, Discovery Life Sciences, Genfit, Gilead, Intercept, Novartis, Novo Nordisk, Orasure Technologies, Ortho Diagnostics, Pfizer, Prometheus Lab, R | Full Paper |
2021 | Varlet, P;Bouffet, E;Casanova, M;Giangaspero, F;Antonelli, M;Hargrave, D;Ladenstein, R;Pearson, A;Hawkins, C;König, FB;Rüschoff, J;Schmauch, C;Bühnemann, C;Garin-Chesa, P;Schweifer, N;Uttenreuther-Fischer, M;Gibson, N;Ittrich, C;Krämer, N;Solca, F;Stolze, B;Geoerger, B; | Comprehensive analysis of the ErbB receptor family in pediatric nervous system tumors and rhabdomyosarcoma | Pediatric blood & cancer | 34546642 | Institute, Medical University, Vienna, Austria 8 Paediatric Drug Development, Children and Young People's Unit, Royal Marsden Hospital, London, UK 9 Division of Clinical Studies, Institute of Cancer Research, London, UK 10 Targos Molecular Pathology | Full Paper |
2021 | Bio, S;Nunes, B; | Twists and turns of an oyster's life: effects of different depuration periods on physiological biochemical functions of oysters | Environmental science and pollution research international | 33559825 | Aquaculture activities are often established in the vicinity of highly populated, potentially contaminated areas. Animals cultured at such locations, namely bivalves, are frequently used as test organisms in ecotoxicological testing. In this case, a | Full Paper |
2021 | Chen, YC;Figliozzi, RW;Hsia, SV; | Pilot Analyses of Interferon Subtype Expression Profiles in Patients with Herpes Zoster or Postherpetic Neuralgia | Viral immunology | 33857386 | A variety of blood samples containing ICD10 codes of HZ or PHN (Table 1) were purchased from Discovery Life Sciences (800 Hudson Way, Suite 1700, Huntsville, AL 35806) The sera of healthy controls were purchased from Discovery Life Sciences with an | Full Paper |
2021 | Kam, K;Kumar, V;Lamport, Z;Kymissis, I; | A laboratory course on information display technologies for remote learning | Journal of the Society for Information Display | Survey response percentage, 55.55%. TABLE 2. Listed components and suppliers Component, Brand. AC/DC wall mount adapter 12 V 12 W, Qualtek. LED matrix 5 × 7, Kingbright. Fan axial 40 × 10mm 12VDC wire, CUI Devices. Switch push, C&K | Full Paper | |
2021 | Rüschoff, J;Baretton, G;Bläker, H;Dietmaier, W;Dietel, M;Hartmann, A;Horn, LC;Jöhrens, K;Kirchner, T;Knüchel, R;Mayr, D;Merkelbach-Bruse, S;Schildhaus, HU;Schirmacher, P;Tiemann, M;Tiemann, K;Weichert, W;Büttner, R; | MSI testing : What's new? What should be considered? | Der Pathologe | 34477921 | Institute of Pathology, Nordhessen und Targos Molecular Pathology GmbH, Germaniastr. 7, 34119, Kassel, Germany. josef.rueschoff@targos-gmbh.de.++Institute of Pathology, University Hospital Carl Gustav Carus, Fetscherstr. 74, 01307, Dresden, Germany.+ | Full Paper |
2021 | Rüschoff, J;Baretton, G;Bläker, H;Dietmaier, W;Dietel, M;Hartmann, A;Horn, LC;Jöhrens, K;Kirchner, T;Knüchel, R;Mayr, D;Merkelbach-Bruse, S;Schildhaus, HU;Schirmacher, P;Tiemann, M;Tiemann, K;Weichert, W;Büttner, R; | [MSI testing : What is new? What should be considered? German version] | Der Pathologe | 34043067 | What should be considered? German version]. Josef Ruschoff Institut fur Pathologie Nordhessen, TARGOS Molecular Pathology GmbH, Germaniastr Affiliations. 1 author 1. Institut fur Pathologie Nordhessen, TARGOS Molecular Pathology GmbH, Germaniastr | Full Paper |
2021 | Schildhaus, HU;Weichert, W; | [Predictive diagnostics for checkpoint inhibitors] | Der Pathologe | 33956171 | H.-U. Schildhaus ist Mitarbeiter der Targos Molecular Pathology GmbH und gibt Honorare und Advisory Boards von MSD, BMS, AstraZeneca, Pfizer, | Full Paper |
2021 | Cromer, MK;Camarena, J;Martin, RM;Lesch, BJ;Vakulskas, CA;Bode, NM;Kurgan, G;Collingwood, MA;Rettig, GR;Behlke, MA;Lemgart, VT;Zhang, Y;Goyal, A;Zhao, F;Ponce, E;Srifa, W;Bak, RO;Uchida, N;Majeti, R;Sheehan, VA;Tisdale, JF;Dever, DP;Porteus, MH; | Gene replacement of α-globin with β-globin restores hemoglobin balance in β-thalassemia-derived hematopoietic stem and progenitor cells | Nature medicine | 33737751 | Human CD34+ HSPCs were cultured as previously described18,24,33,36,46,47. CD34+ HSPCs were sourced from fresh cord blood (generously provided by the Binns Family program for Cord Blood Research); from frozen cord blood and Plerixafor- and/or G-CSF-mo | Full Paper |
2021 | Gaidt, MM;Morrow, A;Fairgrieve, MR;Karr, JP;Yosef, N;Vance, RE; | Self-guarding of MORC3 enables virulence factor-triggered immunity | Nature | 34759314 | (PBMCs) from de-identified donors were obtained from AllCells (Alameda) under donor-informed consent and Alpha IRB approval obtained by AllCells (7000-SOP-045) for the study ' Save | Full Paper |
2021 | Mimitou, EP;Lareau, CA;Chen, KY;Zorzetto-Fernandes, AL;Hao, Y;Takeshima, Y;Luo, W;Huang, TS;Yeung, BZ;Papalexi, E;Thakore, PI;Kibayashi, T;Wing, JB;Hata, M;Satija, R;Nazor, KL;Sakaguchi, S;Ludwig, LS;Sankaran, VG;Regev, A;Smibert, P; | Scalable, multimodal profiling of chromatin accessibility, gene expression and protein levels in single cells | Nature biotechnology | 34083792 | Cryopreserved healthy donor peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BM) were obtained from AllCells (USA) or Cellular Technology Limited (CTL) and processed immediately after thawing. NIH-3T3 and HEK293FT cells were maintain | Full Paper |
2021 | Lareau, CA;Ludwig, LS;Muus, C;Gohil, SH;Zhao, T;Chiang, Z;Pelka, K;Verboon, JM;Luo, W;Christian, E;Rosebrock, D;Getz, G;Boland, GM;Chen, F;Buenrostro, JD;Hacohen, N;Wu, CJ;Aryee, MJ;Regev, A;Sankaran, VG; | Massively parallel single-cell mitochondrial DNA genotyping and chromatin profiling | Nature biotechnology | 32788668 | Cryopreserved PBMCs from patients with CLL consented on institutional review board approved protocols were obtained from AllCells (Patient 1) or from Adrian Wiestner at the Save | Full Paper |
2021 | Hao, Y;Hao, S;Andersen-Nissen, E;Mauck, WM;Zheng, S;Butler, A;Lee, MJ;Wilk, AJ;Darby, C;Zager, M;Hoffman, P;Stoeckius, M;Papalexi, E;Mimitou, EP;Jain, J;Srivastava, A;Stuart, T;Fleming, LM;Yeung, B;Rogers, AJ;McElrath, JM;Blish, CA;Gottardo, R;Smibert, P;Satija, R; | Integrated analysis of multimodal single-cell data | Cell | 34062119 | stom made clone UCHL1 ------------------------- Biological samples ------------------------- Human PBMC Cape Town HVTN Immunology Lab, South Africa https://www.chil.org.za/ Human PBMC AllCells Lot 3032552 ------------------------- | Full Paper |
2021 | Okuchi, Y;Reeves, J;Ng, SS;Doro, DH;Junyent, S;Liu, KJ;El Haj, AJ;Habib, SJ; | Wnt-modified materials mediate asymmetric stem cell division to direct human osteogenic tissue formation for bone repair | Nature materials | 32958876 | hSSCs were isolated from a commercially available bone marrow aspirate (AllCells). The bone marrow aspirate was plated onto culture flasks precoated with 10 ng ml−1 fibronectin (Sigma-Aldrich, 356000) and the cells were allowed to attach. Surface mar | Full Paper |
2021 | Wu, SZ;Al-Eryani, G;Roden, DL;Junankar, S;Harvey, K;Andersson, A;Thennavan, A;Wang, C;Torpy, JR;Bartonicek, N;Wang, T;Larsson, L;Kaczorowski, D;Weisenfeld, NI;Uytingco, CR;Chew, JG;Bent, ZW;Chan, CL;Gnanasambandapillai, V;Dutertre, CA;Gluch, L;Hui, MN;Beith, J;Parker, A;Robbins, E;Segara, D;Cooper, C;Mak, C;Chan, B;Warrier, S;Ginhoux, F;Millar, E;Powell, JE;Williams, SR;Liu, XS;O'Toole, S;Lim, E;Lundeberg, J;Perou, CM;Swarbrick, A; | A single-cell and spatially resolved atlas of human breast cancers | Nature genetics | 34493872 | Percentage of neoplastic cells in each tumor that are classified as each of the SCSubtypes. Tumor samples are grouped according to their Allcells-Pseudobulk classifications. NL, normal-like. c, Representative images of CK5 (top) and ER (bottom) IHC f | Full Paper |
2021 | Ge, Y;Tian, T;Huang, S;Wan, F;Li, J;Li, S;Wang, X;Yang, H;Hong, L;Wu, N;Yuan, E;Luo, Y;Cheng, L;Hu, C;Lei, Y;Shu, H;Feng, X;Jiang, Z;Wu, Y;Chi, Y;Guo, X;Cui, L;Xiao, L;Li, Z;Yang, C;Miao, Z;Chen, L;Li, H;Zeng, H;Zhao, D;Zhu, F;Shen, X;Zeng, J; | An integrative drug repositioning framework discovered a potential therapeutic agent targeting COVID-19 | Signal transduction and targeted therapy | 33895786 | e performed at a flow rate of 30 μL/min. Data processing and analyses were performed using BIAevaluation 1.1 software. LPS-induced cytokine production in PBMCs Peripheral blood mononuclear cells (Allcells) were cultured at 37 °C at concentration 5% | Full Paper |
2021 | Miller, IC;Zamat, A;Sun, LK;Phuengkham, H;Harris, AM;Gamboa, L;Yang, J;Murad, JP;Priceman, SJ;Kwong, GA; | Enhanced intratumoural activity of CAR T cells engineered to produce immunomodulators under photothermal control | Nature biomedical engineering | 34385695 | Primary human CD3+ cells were obtained from an anonymous donor blood after apheresis (AllCells) and were cryopreserved in 90% FBS and 10% dimethylsulfoxide until subsequent Save | Full Paper |
2021 | Frede, J;Anand, P;Sotudeh, N;Pinto, RA;Nair, MS;Stuart, H;Yee, AJ;Vijaykumar, T;Waldschmidt, JM;Potdar, S;Kloeber, JA;Kokkalis, A;Dimitrova, V;Mann, M;Laubach, JP;Richardson, PG;Anderson, KC;Raje, NS;Knoechel, B;Lohr, JG; | Dynamic transcriptional reprogramming leads to immunotherapeutic vulnerabilities in myeloma | Nature cell biology | 34675390 | registration was 68.5 years (range 45-80). Myeloma cells were enriched using the EasySep Human CD138 positive selection kit (STEMCELL Technologies). Bone marrow from normal donors was obtained from AllCells. Single cell sorting Bone marrow cells wer | Full Paper |
2021 | Duault, C;Kumar, A;Taghi Khani, A;Lee, SJ;Yang, L; | Activated natural killer cells predict poor clinical prognosis in high-risk B-and T-cell acute lymphoblastic leukemia | blood | 36107453 | processed from patients with B/T-ALL and healthy donors according to Institutional Review Board policies. Healthy BMMCs were purchased from AllCells (Alameda, CA). Healthy PBMCs were isolated from buffy coats procured from Stanford Blood Center, City | Full Paper |
2021 | Duault, C;Kumar, A;Taghi Khani, A;Lee, SJ;Yang, L;Huang, M;Hurtz, C;Manning, B;Ghoda, L;McDonald, T;Lacayo, NJ;Sakamoto, KM;Carroll, M;Tasian, SK;Marcucci, G;Yu, J;Caligiuri, MA;Maecker, HT;Swaminathan, S; | Activated natural killer cells predict poor clinical prognosis in high-risk B- and T-cell acute lymphoblastic leukemia | Blood | 34077953 | ripheral blood mononuclear cells (PBMCs) were collected and processed from patients with B/T-ALL and healthy donors according to Institutional Review Board policies. Healthy BMMCs were purchased from AllCells (Alameda, CA). Healthy PBMCs were isolate | Full Paper |
2021 | Alavi, A;Krishnamurti, L;Abedi, M;Galeon, I;Reiner, D;Smith, S;Wang, L;Ramezi, A;Rendo, P;Walters, M; | Preliminary Safety and Efficacy Results from Precizn-1: An Ongoing Phase 1/2 Study on Zinc Finger Nuclease-Modified Autologous CD34+ HSPCs for Sickle Cell Disease (SCD) | Blood | Introduction Sickle cell disease (SCD) is caused by pathologic variants in both alleles of the β-globin gene, affecting ~100,000 patients in the US (Strouse. Handb Clin Neurol 2016;138:311-24). Elevated fetal hemoglobin (HbF) levels am | Full Paper | |
2021 | Kwiatkowski, J;Locatelli, F;Walters, M;Porter, J;Hongeng, S;Yannaki, E;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Guo, R;Colvin, R;Gruppioni, K;Thompson, A; | Improvement in Health-Related Quality of Life Following Treatment with Betibeglogene Autotemcel in Patients with Transfusion-Dependent β-Thalassemia Enrolled in Phase 3 Studies | Blood | Introduction: Patients with transfusion-dependent β-thalassemia (TDT) have increased morbidity and mortality and reduced health-related quality of life (HRQoL) compared with the general population owing to the mental, physical, and time demands of ch | Full Paper | |
2021 | Hall, A;Winestone, L;Sullivan, E;Wu, Q;Lamble, A;Walters, M;Aguayo-Hiraldo, P;Baez Conde, L;Coker, T;Dave, H;Dornsife, D;Keating, A;Merino, D;Ramsey, B;Park, J;Agrawal, A; | Access to CAR-T Cell Therapy in Underrepresented Populations: A Multicenter Cohort Study of Pediatric and Young Adult ALL Patients | Blood | Introduction: Social determinants of health such as race, ethnicity, and socioeconomic status (SES) are associated with inferior outcomes in patients with B-cell acute lymphoblastic leukemia (B-ALL). Latinx patients have worse event-free survival and | Full Paper | |
2021 | Thompson, A;Locatelli, F;Yannaki, E;Walters, M;Porter, J;Hongeng, S;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Cavazzana, M;Rasko, J;Du, L;Colvin, R;Kwiatkowski, J; | Restoring Iron Homeostasis in Pts Who Achieved Transfusion Independence after Treatment with Betibeglogene Autotemcel Gene Therapy: Results from up to 7 Years of Follow-up | Blood | Introduction: Transfusion-dependent β-thalassemia (TDT) is a severe genetic disease requiring lifelong, regular packed red blood cell (pRBC) transfusions and iron chelation treatment. Betibeglogene autotemcel (beti-cel) is a one-time ex vivo gene the | Full Paper | |
2021 | Walters, M;Smith, A;Schiller, G;Esrick, E;Williams, D;Gogoleva, T;Rouy, D;Cockroft, B;Vercellotti, G; | Updated Results of a Phase 1/2 Clinical Study of Zinc Finger Nuclease-Mediated Editing of BCL11A in Autologous Hematopoietic Stem Cells for Transfusion-Dependent Beta Thalassemia | Blood | Introduction: Persistently elevated fetal hemoglobin (HbF) can ameliorate transfusion-dependent beta thalassemia (TDT) (Musallam et al ., 2012). BCL11A is a repressor of γ-globin expression and HbF production in adult erythrocytes. Down-regulation of | Full Paper | |
2021 | Tisdale, J;Thompson, A;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Schmidt, M;Pierciey, F;Whitney, D;Rogers, C;Nnamani, M;Foos, M;Miller, A;Zhang, X;Lynch, J;Walters, M;Kanter, J;Bonner, M; | Polyclonality Strongly Correlates with Biological Outcomes and Is Significantly Increased Following Improvements to the Phase 1/2 HGB-206 Protocol and Manufacturing of LentiGlobin for Sickle Cell Disease (SCD; bb1111) Gene Therapy (GT) | Blood | Background: The ongoing Phase 1/2 HGB-206 study (NCT02140554) of LentiGlobin for SCD (bb1111) GT uses a modified human β-globin gene that expresses an anti-sickling hemoglobin (HbA T87Q). The relationships between biological outcomes, clinical outcom | Full Paper | |
2021 | Walters, M;Tisdale, J;Mapara, M;Krishnamurti, L;Kwiatkowski, J;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Jaroscak, J;Garbinsky, D;Chirila, C;Gallagher, M;Zhang, X;Ho, P;Thompson, A;Kanter, J; | Sustained Improvements in Patient-Reported Quality of Life up to 24 Months Post-Treatment with LentiGlobin for Sickle Cell Disease (bb1111) Gene Therapy | Blood | Background: Sickle cell disease (SCD) is characterized by painful vaso-occlusive events (VOEs), progressive vasculopathy, hemolytic anemia, and organ damage resulting in frequent hospitalizations and decreased quality of life (QoL). The ongoing phase | Full Paper | |
2021 | Kruta, M;Sunshine, MJ;Chua, BA;Fu, Y;Chawla, A;Dillingham, CH;Hidalgo San Jose, L;De Jong, B;Zhou, FJ;Signer, RAJ; | Hsf1 promotes hematopoietic stem cell fitness and proteostasis in response to ex vivo culture stress and aging | Cell stem cell | 34388375 | Human cord blood derived CD34 + HSPCs were purchased from AllCells. Cell number and viability were assessed with a hemocytometer based on trypan blue exclusion. Save | Full Paper |
2021 | Turchiano, G;Andrieux, G;Klermund, J;Blattner, G;Pennucci, V;El Gaz, M;Monaco, G;Poddar, S;Mussolino, C;Cornu, TI;Boerries, M;Cathomen, T; | Quantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-Seq | Cell stem cell | 33626327 | Experimental models: cell lines human CD34+ HSPCs AllCells Cat.# CB008F human CD34+ HSPCs StemCell-Technologies Cat.# 70008.3 K562 N/A RRID:CVCL_0004; authenticated by STR profiling | Full Paper |
2021 | Mondala, PK;Vora, AA;Zhou, T;Lazzari, E;Ladel, L;Luo, X;Kim, Y;Costello, C;MacLeod, AR;Jamieson, CHM;Crews, LA; | Selective antisense oligonucleotide inhibition of human IRF4 prevents malignant myeloma regeneration via cell cycle disruption | Cell stem cell | 33476575 | In multiple myeloma, inflammatory and anti-viral pathways promote disease progression and cancer stem cell generation. Using diverse pre-clinical models, we investigated the role of Save | Full Paper |
2021 | Su, M;Lin, Y;Cui, C;Tian, X;Lai, L; | ERMAP is a B7 family-related molecule that negatively regulates T cell and macrophage responses | Cellular & molecular immunology | 32620788 | T cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan-T labeling system were purchased from ALLCELLS, LLC (Alameda, CA). Murine CD3+ T cells were purified from C57BL/6 mice by an immunomagnetic system (Milt | Full Paper |
2021 | Seo, H;Masuda, H;Asagoshi, K;Uchiki, T;Kawata, S;Sasaki, G;Yabuki, T;Miyai, S;Takahashi, N;Hashimoto, SI;Sawada, A;Takaiwa, A;Koyama, C;Tamai, K;Kurosawa, K;Lin, KY;Ohta, K;Nakazaki, Y; | Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line | Cellular & molecular immunology | 32457406 | The germline-derived divergent hV H harboring CDR3 was prepared from normal human peripheral blood CD19 + B-cell cDNA (ALLCELLS) using degenerate primers that annealed to Save | Full Paper |
2021 | Sadras, T;Martin, M;Kume, K;Robinson, ME;Saravanakumar, S;Lenz, G;Chen, Z;Song, JY;Siddiqi, T;Oksa, L;Knapp, AM;Cutler, J;Cosgun, KN;Klemm, L;Ecker, V;Winchester, J;Ghergus, D;Soulas-Sprauel, P;Kiefer, F;Heisterkamp, N;Pandey, A;Ngo, V;Wang, L;Jumaa, H;Buchner, M;Ruland, J;Chan, WC;Meffre, E;Martin, T;Müschen, M; | Developmental partitioning of SYK and ZAP70 prevents autoimmunity and cancer | Molecular cell | 33878293 | Cord blood derived human CD34 + cells (ALLCELLS) were thawed and were lentivirally transduced with pCL6-ZAP70-GFP or an empty vector control. To obtain optimal transduction, Save | Full Paper |
2021 | Lattanzi, A;Camarena, J;Lahiri, P;Segal, H;Srifa, W;Vakulskas, CA;Frock, RL;Kenrick, J;Lee, C;Talbott, N;Skowronski, J;Cromer, MK;Charlesworth, CT;Bak, RO;Mantri, S;Bao, G;DiGiusto, D;Tisdale, J;Wright, JF;Bhatia, N;Roncarolo, MG;Dever, DP;Porteus, MH; | Development of β-globin gene correction in human hematopoietic stem cells as a potential durable treatment for sickle cell disease | Science translational medicine | 34135108 | Human CD34+ HSPCs were cultured in small-scale conditions as previously described (67, 20, 25, 68, 69). Frozen purified CD34+ HSPCs were sourced from plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA and STEMCELL Technol | Full Paper |
2021 | Jain, MS;Polanski, K;Conde, CD;Chen, X;Park, J;Mamanova, L;Knights, A;Botting, RA;Stephenson, E;Haniffa, M;Lamacraft, A;Efremova, M;Teichmann, SA; | MultiMAP: dimensionality reduction and integration of multimodal data | Genome biology | 34930412 | sed to identify genes whose expression changes significantly along the trajectory. Acquisition and processing of human PBMCs PBMCs from two donors were acquired from a LeukoLab (Clinical division of AllCells). Frozen PBMC samples were thawed quickly | Full Paper |
2021 | Thongon, N;Ma, F;Santoni, A;Marchesini, M;Fiorini, E;Rose, A;Adema, V;Ganan-Gomez, I;Groarke, EM;Gutierrez-Rodrigues, F;Chen, S;Lockyer, P;Schneider, S;Bueso-Ramos, C;Montalban-Bravo, G;Class, CA;Soltysiak, KA;Pellegrini, M;Sahin, E;Bertuch, AA;DiNardo, CD;Garcia-Manero, G;Young, NS;Dwyer, K;Colla, S; | Hematopoiesis under telomere attrition at the single-cell resolution | Nature communications | 34824242 | BM samples from HDs were obtained from AllCells. Written informed consent was obtained from all donors. The clinical characteristics and the PB counts of the individuals with Save | Full Paper |
2021 | Qiao, Y;Qiu, Y;Ding, J;Luo, N;Wang, H;Ling, X;Sun, J;Wu, Z;Wang, Y;Liu, Y;Guo, F;Sun, T;Shen, W;Zhang, M;Wu, D;Chen, B;Xu, W;Wang, X; | Cancer immune therapy with PD-1-dependent CD137 co-stimulation provides localized tumour killing without systemic toxicity | Nature communications | 34737267 | Human PBMCs were purchased from Allcells and the informed consent was obtained from all donors. Human PBMCs were cultured in RPMI 1640 medium supplemented with 10% FBS Save | Full Paper |
2021 | Duren, Z;Lu, WS;Arthur, JG;Shah, P;Xin, J;Meschi, F;Li, ML;Nemec, CM;Yin, Y;Wong, WH; | Sc-compReg enables the comparison of gene regulatory networks between conditions using single-cell data | Nature communications | 34362918 | itial analysis has considered the HiChIP data. We will extend our sc-compReg software to enable this type of analysis. Methods Single-cell ATAC Cryopreserved BMMCs from two donors were obtained from AllCells. One donor was a 93-year-old non-Hispanic | Full Paper |
2021 | Amit, I;Iancu, O;Levy-Jurgenson, A;Kurgan, G;McNeill, MS;Rettig, GR;Allen, D;Breier, D;Ben Haim, N;Wang, Y;Anavy, L;Hendel, A;Yakhini, Z; | CRISPECTOR provides accurate estimation of genome editing translocation and off-target activity from comparative NGS data | Nature communications | 34031394 | ubation at room temperature before the electroporation. Genome editing in mobilized CD34+ HSPCs and in HEK293/HEK293-Cas9 cell lines Human CD34+ HSPCs, derived from mobilized peripheral blood (MPB) (AllCells, Alameda, CA), were thawed and cultured f | Full Paper |
2021 | Lal, A;Chiang, ZD;Yakovenko, N;Duarte, FM;Israeli, J;Buenrostro, JD; | Deep learning-based enhancement of epigenomics data with AtacWorks | Nature communications | 33686069 | 0 or 1 representing whether that position was part of a CTCF binding motif on the reverse strand. Generation of dscATAC-seq data for FACS-isolated HSCs Cryopreserved human BMMCs were purchased from Allcells (catalog number BM, CR, MNC, 10 M). Cells | Full Paper |
2021 | Sharma, R;Dever, DP;Lee, CM;Azizi, A;Pan, Y;Camarena, J;Köhnke, T;Bao, G;Porteus, MH;Majeti, R; | The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells | Nature communications | 33473139 | For in vivo studies presented, cord blood-derived CD34 + cells were purchased from AllCells or STEMCELL Technologies and were thawed according to the manufacturer's Save | Full Paper |
2021 | Yang, KD;Belyaeva, A;Venkatachalapathy, S;Damodaran, K;Katcoff, A;Radhakrishnan, A;Shivashankar, GV;Uhler, C; | Multi-domain translation between single-cell imaging and sequencing data using autoencoders | Nature communications | 33397893 | nly limited functional data is available by acquiring chromatin imaging data. Methods Cell culture and immunostaning CD4+/CD45RA+ naive helper T-cells from human peripheral blood were purchased from AllCells. These cells were revived and cultured in | Full Paper |
2021 | Gnanapragasam, M;Planutis, A;Glassberg, J;Bieker, J; | Identification of a genomic DNA sequence that quantitatively modulates KLF1 expression in differentiating human hematopoietic cells | bioRxiv | CD34+ cells were purchased from AllCells or obtained from the Yale Cooperative Center of Excellence in Hematology. These were differentiated under three-phase protocols, initially based on (Antoniani et al., 2018; Yien and Bieker, 2012) but then late | Full Paper | |
2021 | Shy, B;Vykunta, V;Ha, A;Roth, T;Talbot, A;Nguyen, D;Chen, Y;Blaeschke, F;Vedova, S;Mamedov, M;Chung, J;Li, H;Wolf, J;Martin, T;Ye, L;Eyquem, J;Esensten, J;Marson, A; | Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing | bioRxiv | Primary adult blood cells were obtained from anonymous healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc. or Allcells Inc, or as a Trima residual from Vitalant. If needed, peripheral blood mononuclear cells were i | Full Paper | |
2021 | Amin, OE;Colbeck, EJ;Daffis, S;Khan, S;Ramakrishnan, D;Pattabiraman, D;Chu, R;Micolochick Steuer, H;Lehar, S;Peiser, L;Palazzo, A;Frey, C;Davies, J;Javanbakht, H;Rosenberg, WMC;Fletcher, SP;Maini, MK;Pallett, LJ; | Therapeutic Potential of TLR8 Agonist GS-9688 (Selgantolimod) in Chronic Hepatitis B: Remodeling of Antiviral and Regulatory Mediators | Hepatology (Baltimore, Md.) | 33368377 | In select experiments, whole blood was obtained from AllCells (Alameda, CA) and the MT Group (Van Nuys, CA). In this instance, consent was obtained from the donor or donor's legal Save | Full Paper |
2021 | Shukla, S;Ying, W;Gray, F;Yao, Y;Simes, ML;Zhao, Q;Miao, H;Cho, HJ;González-Alonso, P;Winkler, A;Lund, G;Purohit, T;Kim, E;Zhang, X;Ray, JM;He, S;Nikolaidis, C;Ndoj, J;Wang, J;Jaremko, Ł;Jaremko, M;Ryan, RJH;Guzman, ML;Grembecka, J;Cierpicki, T; | Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain | Nature chemical biology | 34155404 | CD34-positive bone marrow blood cells were obtained from Allcells (ABM021F) and stored in liquid nitrogen until use. For treatment experiments that lasted more than 4 d, media were Save | Full Paper |
2021 | Jang, S;Song, J;Kim, N;Bak, J;Jung, K;Park, YW;Park, BC;Kim, HM; | Development of an antibody-like T-cell engager based on VH-VL heterodimer formation and its application in cancer therapy | Biomaterials | 33774526 | All healthy donor PBMCs and CD8 + T cells used in this study were purchased from AllCells (#PB004F and #PB009-3 F), Zenbio (#SER-PBMC-200-F), and Lonza (#3W-270). CD3 + T Save | Full Paper |
2021 | Davis-Marcisak, EF;Fitzgerald, AA;Kessler, MD;Danilova, L;Jaffee, EM;Zaidi, N;Weiner, LM;Fertig, EJ; | Transfer learning between preclinical models and human tumors identifies a conserved NK cell activation signature in anti-CTLA-4 responsive tumors | Genome medicine | 34376232 | Fresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selected from donor peripheral blood using CD56 positivity. Donor NK cell purity Save | Full Paper |
2021 | Steinbuck, MP;Seenappa, LM;Jakubowski, A;McNeil, LK;Haqq, CM;DeMuth, PC; | A lymph node-targeted Amphiphile vaccine induces potent cellular and humoral immunity to SARS-CoV-2 | Science advances | 33547083 | scent serum/plasma Convalescent serum samples (n = 7) and plasma samples (n = 15) from patients who had recovered from SARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and AllCells (Alameda, CA), respectively. All samples | Full Paper |
2021 | Lin, Y;Cui, C;Su, M;Silbart, LK;Liu, H;Zhao, J;He, L;Huang, Y;Xu, D;Wei, X;Du, Q;Lai, L; | Identification of TAPBPL as a novel negative regulator of T-cell function | EMBO molecular medicine | 33938620 | itro T‐cell assays Normal human peripheral blood CD3+ Pan T cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan‐T labeling system were purchased from ALLCELLS, LLC (Alameda, CA). Murine CD3+ T cells were pu | Full Paper |
2021 | Kim, WT;Hennick, K;Johnson, J;Finnerty, B;Choo, S;Short, SB;Drubin, C;Forster, R;McMaster, ML;Hockemeyer, D; | Cancer-associated POT1 mutations lead to telomere elongation without induction of a DNA damage response | The EMBO journal | 33934394 | e aging or age‐associated diseases (Chakravarti et al, 2021). Materials and Methods Cell culture and cryopreservation Human bone marrow CD34+ hematopoietic stem/progenitor cells were obtained from AllCells and Fred Hutchinson Cancer Research Center. | Full Paper |
2021 | Deegen, P;Thomas, O;Nolan-Stevaux, O;Li, S;Wahl, J;Bogner, P;Aeffner, F;Friedrich, M;Liao, MZ;Matthes, K;Rau, D;Rattel, B;Raum, T;Kufer, P;Coxon, A;Bailis, JM; | The PSMA-targeting Half-life Extended BiTE Therapy AMG 160 has Potent Antitumor Activity in Preclinical Models of Metastatic Castration-resistant Prostate Cancer | Clinical cancer research : an official journal of the American Association for Cancer Research | 33504551 | Human T cells were obtained from AllCells. Human peripheral blood mononuclear cells (PBMC) were isolated from buffy coats collected at the Institute of Clinical Transfusion Medicine Save | Full Paper |
2021 | Giffin, MJ;Cooke, K;Lobenhofer, EK;Estrada, J;Zhan, J;Deegen, P;Thomas, M;Murawsky, CM;Werner, J;Liu, S;Lee, F;Homann, O;Friedrich, M;Pearson, JT;Raum, T;Yang, Y;Caenepeel, S;Stevens, J;Beltran, PJ;Canon, J;Coxon, A;Bailis, JM;Hughes, PE; | AMG 757, a Half-Life Extended, DLL3-Targeted Bispecific T-Cell Engager, Shows High Potency and Sensitivity in Preclinical Models of Small-Cell Lung Cancer | Clinical cancer research : an official journal of the American Association for Cancer Research | 33203642 | human CD3+ T cells (2 × 107) by intravenous infusion on study day 0. Human CD3+ T cells, isolated from PBMCs from two different donors using negative selection, were purchased from AllCells and activated in vitro using IL2 and CD3/CD28/CD2 (STEMCELL | Full Paper |
2021 | Kögler, AC;Kherdjemil, Y;Bender, K;Rabinowitz, A;Marco-Ferreres, R;Furlong, EEM; | Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryos | Developmental cell | 34363757 | were also taken from regions not overlapping any mCherry-expressing cells (background (BG)) and from regions overlapping the entire group of cells investigated (AllCells) to Save;each time point, the same measurements were also taken from regions n | Full Paper |
2021 | Montalban-Bravo, G;Kanagal-Shamanna, R;Darbaniyan, F;Siddiqui, MT;Sasaki, K;Wei, Y;Yang, H;Chien, KS;Naqvi, K;Jabbour, E;Kadia, TM;Daver, N;DiNardo, C;Ravandi, F;Pemmaraju, N;Bose, P;Verstovsek, S;Pierce, S;Bueso-Ramos, C;Patel, K;Do, KA;Kantarjian, H;Garcia-Manero, G; | Clinical, genomic, and transcriptomic differences between myelodysplastic syndrome/myeloproliferative neoplasm with ring sideroblasts and thrombocytosis (MDS/MPN-RS-T) and myelodysplastic syndrome with ring sideroblasts (MDS-RS) | American journal of hematology | 33811786 | seven patients with MDS-RS, and 17 healthy individuals obtained from AllCells (Emeryville, CA) were isolated using the CD34 MicroBead Kit and RNA was isolated using the PicoPure RNA isolation kit, following manufacturer's instructions. Fastq files we | Full Paper |
2021 | Kwee, BJ;Lam, J;Akue, A;KuKuruga, MA;Zhang, K;Gu, L;Sung, KE; | Functional heterogeneity of IFN-γ-licensed mesenchymal stromal cell immunosuppressive capacity on biomaterials | Proceedings of the National Academy of Sciences of the United States of America | 34446555 | MSCs obtained from AllCells and Lonza cultured on the two hydrogels (SI Appendix Overall similar observations were obtained with a representative MSC line obtained from AllCells ( Save | Full Paper |
2021 | Cui, L;Moraga, I;Lerbs, T;Van Neste, C;Wilmes, S;Tsutsumi, N;Trotman-Grant, AC;Gakovic, M;Andrews, S;Gotlib, J;Darmanis, S;Enge, M;Quake, S;Hitchcock, IS;Piehler, J;Garcia, KC;Wernig, G; | Tuning MPL signaling to influence hematopoietic stem cell differentiation and inhibit essential thrombocythemia progenitors | Proceedings of the National Academy of Sciences of the United States of America | 33384332 | Healthy deidentified human bone marrow samples were acquired from AllCells. Essential thrombocytosis bone marrow samples were deidentified and acquired from the Stanford Save | Full Paper |
2021 | Poussin, M;Sereno, A;Wu, X;Huang, F;Manro, J;Cao, S;Carpenito, C;Glasebrook, A;Powell, DJ;Demarest, S; | Dichotomous impact of affinity on the function of T cell engaging bispecific antibodies | Journal for immunotherapy of cancer | 34253637 | w cytometric evaluation of cell surface proteins on T cells 48 hours after mixing SKOV3 cells and biologic test articles is further described in online supplemental methods. Briefly, human T cells (ALLCELLS frozen normal human peripheral blood CD3+ P | Full Paper |
2021 | Roselli, E;Boucher, JC;Li, G;Kotani, H;Spitler, K;Reid, K;Cervantes, EV;Bulliard, Y;Tu, N;Lee, SB;Yu, B;Locke, FL;Davila, ML; | 4-1BB and optimized CD28 co-stimulation enhances function of human mono-specific and bi-specific third-generation CAR T cells | Journal for immunotherapy of cancer | 34706886 | Human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells. T cells were enriched from PBMCs using the EasySep human T cell isolation kit according to Save | Full Paper |
2021 | Paavola, KJ;Roda, JM;Lin, VY;Chen, P;O'Hollaren, KP;Ventura, R;Crawley, SC;Li, B;Chen, HH;Malmersjö, S;Sharkov, NA;Horner, G;Guo, W;Kutach, AK;Mondal, K;Zhang, Z;Lichtman, JS;Song, C;Rivera, LB;Liu, W;Luo, J;Wang, Y;Solloway, MJ;Allan, BB;Kekatpure, A;Starck, SR;Haldankar, R;Fan, B;Chu, C;Tang, J;Molgora, M;Colonna, M;Kaplan, DD;Hsu, JY; | The Fibronectin-ILT3 Interaction Functions as a Stromal Checkpoint that Suppresses Myeloid Cells | Cancer immunology research | 34426457 | Human peripheral blood mononuclear cells (PBMC) of healthy donors were isolated from freshly drawn leukopaks (Allcells, catalog no. PB007) by density gradient centrifugation over Save | Full Paper |
2021 | Badeaux, MD;Rolig, AS;Agnello, G;Enzler, D;Kasiewicz, MJ;Priddy, L;Wiggins, JF;Muir, A;Sullivan, MR;Van Cleef, J;Daige, C;Vander Heiden, MG;Rajamanickam, V;Wooldridge, JE;Redmond, WL;Rowlinson, SW; | Arginase Therapy Combines Effectively with Immune Checkpoint Blockade or Agonist Anti-OX40 Immunotherapy to Control Tumor Growth | Cancer immunology research | 33500272 | Primary human bone marrow (AllCells) was obtained from healthy patients and cultured as follows. Briefly, Hs5 cells were cultured for 72 hours in SFEM2 media (Stem Cell Save | Full Paper |
2021 | Boucher, JC;Li, G;Kotani, H;Cabral, ML;Morrissey, D;Lee, SB;Spitler, K;Beatty, NJ;Cervantes, EV;Shrestha, B;Yu, B;Kazi, A;Wang, X;Sebti, SM;Davila, ML; | CD28 Costimulatory Domain-Targeted Mutations Enhance Chimeric Antigen Receptor T-cell Function | Cancer immunology research | 33188139 | An obstacle to the development of chimeric antigen receptor (CAR) T cells is the limited understanding of CAR T-cell biology and the mechanisms behind their antitumor activity. We and Save | Full Paper |
2021 | Li, J;Whelan, S;Kotturi, MF;Meyran, D;D'Souza, C;Hansen, K;Liang, S;Hunter, J;Trapani, JA;Neeson, PJ; | PVRIG is a novel natural killer cell immune checkpoint receptor in acute myeloid leukemia | Haematologica | 33147937 | nt clinical characteristics are summarised in the Online Supplementary Table S2. Healthy donor bone marrow samples were obtained from Royal Melbourne Hospital (Melbourne, Australia) or purchased from AllCells (Alameda, California). All bone marrow sa | Full Paper |
2021 | Gilmartin, AG;Groy, A;Gore, ER;Atkins, C;Long, ER;Montoute, MN;Wu, Z;Halsey, W;McNulty, DE;Ennulat, D;Rueda, L;Pappalardi, M;Kruger, RG;McCabe, MT;Raoof, A;Butlin, R;Stowell, A;Cockerill, M;Waddell, I;Ogilvie, D;Luengo, J;Jordan, A;Benowitz, AB; | In vitro and in vivo induction of fetal hemoglobin with a reversible and selective DNMT1 inhibitor | Haematologica | 32586904 | Cryopreserved human bone marrow CD34+ cells (AllCells) were confirmed to be sourced ethically, and their research use was in accord with the terms of the informed consents under Save | Full Paper |
2021 | Zhang, C;Zhang, X;Huang, L;Guan, Y;Huang, X;Tian, XL;Zhang, L;Tao, W; | ATF3 drives senescence by reconstructing accessible chromatin profiles | Aging cell | 33539668 | accessibility in IARs. Accordingly, our research provides a target to aim for in the exploration of senescence and aging. 4 EXPERIMENTAL PROCEDURES 4.1 Cell culture We purchased primary HUVECs from AllCells Biotech Shanghai Co., Ltd., and cultured t | Full Paper |
2021 | Nesterenko, PA;McLaughlin, J;Tsai, BL;Burton Sojo, G;Cheng, D;Zhao, D;Mao, Z;Bangayan, NJ;Obusan, MB;Su, Y;Ng, RH;Chour, W;Xie, J;Li, YR;Lee, D;Noguchi, M;Carmona, C;Phillips, JW;Kim, JT;Yang, L;Heath, JR;Boutros, PC;Witte, ON; | HLA-A∗02:01 restricted T cell receptors against the highly conserved SARS-CoV-2 polymerase cross-react with human coronaviruses | Cell reports | 34919800 | Cryo preserved peripheral blood mononuclear cells (PBMCs) were commercially purchased (Allcells and Hemacare) or obtained from the CFAR Virology Core Laboratory at the UCLA Save;d virus strains ------------------------- NFAT-zsGreen virus UCLA, | Full Paper |
2021 | Qian, Y;Arellano, G;Ifergan, I;Lin, J;Snowden, C;Kim, T;Thomas, JJ;Law, C;Guan, T;Balabanov, RD;Kaech, SM;Miller, SD;Choi, J; | ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis | Cell reports | 34433042 | FN-γ, 1 μg/mL anti-IL2 (Cat #554375, clone: S4B6, BD). Isolation of human CD4+ naive T cells and Th cells differentiation Fresh Leukopak samples from de-identified blood donors were purchased from AllCells, LLC. Peripheral blood mononuclear cells (P | Full Paper |
2021 | Hamilton, JR;Tsuchida, CA;Nguyen, DN;Shy, BR;McGarrigle, ER;Sandoval Espinoza, CR;Carr, D;Blaeschke, F;Marson, A;Doudna, JA; | Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering | Cell reports | 34077734 | ons. Isolation and culture of human primary T cells Primary adult blood cells were obtained from anonymous healthy human donors as a leukoreduction pack purchased from StemCell Technologies, Inc. or Allcells Inc, or as a Trima residual from Vitalant | Full Paper |
2021 | Jiang, Q;Isquith, J;Ladel, L;Mark, A;Holm, F;Mason, C;He, Y;Mondala, P;Oliver, I;Pham, J;Ma, W;Reynoso, E;Ali, S;Morris, IJ;Diep, R;Nasamran, C;Xu, G;Sasik, R;Rosenthal, SB;Birmingham, A;Coso, S;Pineda, G;Crews, L;Donohoe, ME;Venter, JC;Whisenant, T;Mesa, RA;Alexandrov, LB;Fisch, KM;Jamieson, C; | Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolution | Cell reports | 33503434 | Aged normal and MPN Patient samples Obtained through patients consenting at UCSD Moores Cancer Center according to Institutional Review Boardapproved protocols. N/A Young Cord blood CD34+ cells Purchased from AllCells or StemCell Technologies | Full Paper |
2021 | Bunis, DG;Bronevetsky, Y;Krow-Lucal, E;Bhakta, NR;Kim, CC;Nerella, S;Jones, N;Mendoza, VF;Bryson, YJ;Gern, JE;Rutishauser, RL;Ye, CJ;Sirota, M;McCune, JM;Burt, TD; | Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells | Cell reports | 33406429 | Matched adult peripheral blood and BM samples were obtained from healthy volunteer donors through AllCells (Alameda, CA). UCB and longitudinal post-natal samples were obtained Save | Full Paper |
2021 | Yang, Z;Huang, Y;Zhu, L;Yang, K;Liang, K;Tan, J;Yu, B; | SIRT6 promotes angiogenesis and hemorrhage of carotid plaque via regulating HIF-1α and reactive oxygen species | Cell death & disease | 33436551 | oCl2) was purchased from Sigma-Aldrich, Inc. (Burlington, MA, USA). The work concentration and time were confirmed by instructions combined with experimental requirements. HUVECs were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured in | Full Paper |
2021 | Yang, Z;Wei, X;Pan, Y;Xu, J;Si, Y;Min, Z;Yu, B; | A new risk factor indicator for papillary thyroid cancer based on immune infiltration | Cell death & disease | 33414407 | d. The sections were fixed and images were obtained with an inverted microscope (Olympus IX71, Japan). Cell culture and co-culture Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured in | Full Paper |
2021 | Everitt, ML;Boegner, DJ;Birukov, KG;White, IM; | Sample-to-Answer Diagnostic System for the Detection of Circulating Histones in Whole Blood | ACS sensors | 34270219 | Varying concentrations of histone octamers (Sigma-Aldrich) (0-65 μg/mL final concentrations) are suspended in either fetal bovine serum (FBS) (ATCC) or whole blood (AllCells) (medium is indicated in figure caption). Fifteen microliters of histone-spi | Full Paper |
2021 | Varricchio, L;Iancu-Rubin, C;Upadhyaya, B;Zingariello, M;Martelli, F;Verachi, P;Clementelli, C;Denis, JF;Rahman, AH;Tremblay, G;Mascarenhas, J;Mesa, RA;O'Connor-McCourt, M;Migliaccio, AR;Hoffman, R; | TGF-β1 protein trap AVID200 beneficially affects hematopoiesis and bone marrow fibrosis in myelofibrosis | JCI insight | 34383713 | n and response to TGF-β. Methods Further information and complete unedited blots can be found in Supplemental Methods. Human subjects. Primary ND specimens were purchased from a commercial source (AllCells, LLC), whereas MF MNCs were provided by th | Full Paper |
2021 | Pan, Y;You, Y;Sun, L;Sui, Q;Liu, L;Yuan, H;Chen, C;Liu, J;Wen, X;Dai, L;Sun, H; | The STING antagonist H-151 ameliorates psoriasis via suppression of STING/NF-κB-mediated inflammation | British journal of pharmacology | 34460100 | Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells Shanghai and were used immediately after resuscitation. H-151 was synthesized according to the published Save | Full Paper |
2021 | Hu, X;Qie, C;Jiang, J;Xie, X;Chen, W;Liu, W;Liu, J; | M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTA | British journal of pharmacology | 33450048 | Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells and were used immediately after resuscitation; these cells cannot be subcultured. Human CD4 + T cells Save | Full Paper |
2021 | Wang, X;Guo, J;Wen, J;Zhang, X;Cao, L;Zeng, D;Liu, X;Jiang, X; | Novel vascular strategies on polyetheretherketone modification in promoting osseointegration in ovariectomized rats | Materials & Design | HUVECs were purchased from AllCells Biology Technology Company (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, Lonza) without endothelial cell growth supplementation. HUVECs at a density of 2 × 104 cells per mL were seeded o | Full Paper | |
2021 | Iftakhar-E-Khuda, I;Pessia, A;Zheng, S;Kankainen, M;Kontro, M;Karikoski, M;Laurila, J;Gerke, H;Tadayon, S;Hollmén, M;Tang, J;Imhof, BA;Salmi, M;Jalkanen, S; | Vascular adhesion protein-1 defines a unique subpopulation of human hematopoietic stem cells and regulates their proliferation | Cellular and molecular life sciences : CMLS | 34719737 | Five hundred cryopreserved and thawed human BM CD34 + cells (AllCells) were cultured in complete methylcellulose-based medium (H4436, STEMCELL Technologies) with or Save | Full Paper |
2021 | Huang, YJ;Cao, J;Lee, CY;Wu, YM; | Umbilical cord blood plasma-derived exosomes as a novel therapy to reverse liver fibrosis | Stem cell research & therapy | 34772443 | Human UCB and peripheral blood (PB) plasma were purchased from AllCells LLC (Alameda, CA), and exosomes were acquired using ExoQuick Exosome isolation reagent (System Save | Full Paper |
2021 | Pieles, O;Reichert, TE;Morsczeck, C; | Classical isoforms of protein kinase C (PKC) and Akt regulate the osteogenic differentiation of human dental follicle cells via both β-catenin and NF-κB | Stem cell research & therapy | 33853677 | to examine the role of classical PKC isoforms and the potential downstream mechanisms during the osteogenic differentiation of DFCs. Materials and methods Cell culture Human DFCs were purchased from AllCells (Emeryville, USA) and grown in Dulbecco’s | Full Paper |
2021 | Lin, Z;Liu, X;Liu, T;Gao, H;Wang, S;Zhu, X;Rong, L;Cheng, J;Cai, Z;Xu, F;Tan, X;Lv, L;Li, Z;Sun, Y;Qian, Q; | Evaluation of Nonviral piggyBac and lentiviral Vector in Functions of CD19chimeric Antigen Receptor T Cells and Their Antitumor Activity for CD19+ Tumor Cells | Frontiers in immunology | 35082789 | Peripheral blood mononuclear cells (PBMCs) from whole blood were purchased from AllCells (Shanghai, China) and isolated by Ficoll density gradient centrifugation (Cat#17-5442-03, Save | Full Paper |
2021 | Cui, X;Jia, H;Xin, H;Zhang, L;Chen, S;Xia, S;Li, X;Xu, W;Chen, X;Feng, Y;Wei, X;Yu, H;Wang, Y;Zhan, Y;Zhu, X;Zhang, X; | A Novel Bispecific Antibody Targeting PD-L1 and VEGF With Combined Anti-Tumor Activities | Frontiers in immunology | 34925354 | Human PBMCs and DCs were acquired by using the Easy MLR Kit (Allcells, Alameda). DCs were treated with mitomycin (H19999025, Hisun Pharm Co., Ltd.) for 30 min before co- Save | Full Paper |
2021 | Ceglia, V;Kelley, EJ;Boyle, AS;Zurawski, S;Mead, HL;Harms, CE;Blanck, JP;Flamar, AL;Kirschman, JH;Ogongo, P;Ernst, JD;Levy, Y;Zurawski, G;Altin, JA; | A Framework to Identify Antigen-Expanded T Cell Receptor Clusters Within Complex Repertoires | Frontiers in immunology | 34917073 | cificity, are described in other studies (16, 17, 19). Cohesin-Influenza Matrix 1 (Flu M1) protein is described in (16). Donors Cryopreserved human PBMC from normal donors were sourced commercially (AllCells, CA. ND1001 ID:A5983, ND1002 ID:9441, ND1 | Full Paper |
2021 | Teo Hansen Selnø, A;Schlichtner, S;Yasinska, IM;Sakhnevych, SS;Fiedler, W;Wellbrock, J;Berger, SM;Klenova, E;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV; | High Mobility Group Box 1 (HMGB1) Induces Toll-Like Receptor 4-Mediated Production of the Immunosuppressive Protein Galectin-9 in Human Cancer Cells | Frontiers in immunology | 34234778 | Medical Centre Hamburg-Eppendorf (Ethik Kommission der Ärztekammer Hamburg, reference: PV3469). Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were also purchased from AllCells (Alameda, CA, USA) and handled in accordanc | Full Paper |
2021 | Xie, X;Chen, C;Chen, W;Jiang, J;Wang, L;Li, T;Sun, H;Liu, J; | Structural Basis of VSIG3: The Ligand for VISTA | Frontiers in immunology | 33841409 | azole buffer (GenScript). Protein was concentrated using 10K MWCO spin columns (Merck). The purified protein was used for functional verification. Cell Culture and Reagents PBMCs were purchased from Allcells Biotechnology (Shanghai) Co., LTD, and we | Full Paper |
2021 | Jost, M;Jacobson, AN;Hussmann, JA;Cirolia, G;Fischbach, MA;Weissman, JS; | CRISPR-based functional genomics in human dendritic cells | eLife | 33904395 | t al., 2018 Referred to as ‘B. theta 4PP’ in the text Mutant producing tetra-acylated, di-phosphorylated lipid A in a capsule-producing background Biological sample (human) PBMCs AllCells Freshly isolated from de-identified healt | Full Paper |
2021 | Gao, X;Guan, M;Liu, X;Xu, H;Huang, Q;Chen, L;Huang, S;Xiao, Y;Shi, X;Lin, Z; | Sustained delivery of growth factors and alendronate using partially demineralized dentin matrix for endogenous periodontal regeneration | Applied Materials Today | cells (HUVECs) were purchased from AllCells (Shanghai, China). HUVECs were cultured in endothelial cell growth medium (EGM; AllCells, Shanghai, China). Angiogenesis Save | Full Paper | |
2021 | Scherer, SD;Riggio, AI;Haroun, F;DeRose, YS;Ekiz, HA;Fujita, M;Toner, J;Zhao, L;Li, Z;Oesterreich, S;Samatar, AA;Welm, AL; | An immune-humanized patient-derived xenograft model of estrogen-independent, hormone receptor positive metastatic breast cancer | Breast cancer research : BCR | 34717714 | CD34 + hematopoietic stem cells (HSCs) isolated from healthy donor bone marrow (BM) were purchased from AllCells. On the day of injection, cells were thawed in a 37 C water bath Save | Full Paper |
2021 | Nada, MH;Wang, H;Hussein, AJ;Tanaka, Y;Morita, CT; | PD-1 checkpoint blockade enhances adoptive immunotherapy by human Vγ2Vδ2 T cells against human prostate cancer | Oncoimmunology | 34712512 | e IgG1 (clone MOPC-21) antibodies were from Bio X Cell (West Lebanon, NH). Ex vivo expansion of Vγ2Vδ2 T cells by pulse zoledronic acid stimulation PBMC were isolated from random donor leukopaks (AllCells, LLC, Alameda, CA) by density centrifugation | Full Paper |
2021 | Jiang, H;Ni, H;Zhang, P;Guo, X;Wu, M;Shen, H;Wang, J;Wu, W;Wu, Z;Ding, J;Tang, R;Zhou, S;Chen, B;Yu, M;Jing, H;Liu, J; | PD-L1/LAG-3 bispecific antibody enhances tumor-specific immunity | Oncoimmunology | 34239776 | oduced by Innovent Biologics Co., Ltd. (Suzhou, China). Human IgG was purchased from Equitech-Bio (Kerrville). Peripheral blood mononuclear cells (PBMCs) and dendritic cells (DCs) were purchased from AllCells (Shanghai, China). Cynomolgus macaque PBM | Full Paper |
2021 | Wang, X;Peticone, C;Kotsopoulou, E;Göttgens, B;Calero-Nieto, FJ; | Single-cell transcriptome analysis of CAR T-cell products reveals subpopulations, stimulation, and exhaustion signatures | Oncoimmunology | 33489472 | or samples PBMCs were derived from healthy donors leukapheresis. Peripheral blood leukapaheresis were obtained from the NHS as part of a research study (IRAS ID 185945) or purchased as LeukoPaks from AllCells. Manufacturing of CAR T cells product Ge | Full Paper |
2021 | Zhang, C;Delawary, M;Huang, P;Korchak, JA;Suda, K;Zubair, AC; | IL-10 mRNA Engineered MSCs Demonstrate Enhanced Anti-Inflammation in an Acute GvHD Model | Cells | 34831324 | g T7 promoter, 5′ UTRs, 3′ UTRs, and poly-A tail. 2.3. MSC Culture and Time Course Experiment Human MSCs were generated from commercial de-identified bone marrow (Catalog# ABM002) purchased from AllCells (Alameda, CA, USA). The donor was a healthy 2 | Full Paper |
2021 | Ahn, Y;An, JH;Yang, HJ;Lee, DG;Kim, J;Koh, H;Park, YH;Song, BS;Sim, BW;Lee, HJ;Lee, JH;Kim, SU; | Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System | Cells | 34440805 | Human Induced Pluripotent Stem Cells (hiPSCs) and Culture Conditions The hiPSCs were reprogrammed from primary human bone marrow cells (Allcells, Emeryville, CA, USA) as Save;The hiPSCs were reprogrammed from primary human bone marrow cells (Allcel | Full Paper |
2021 | Dogan, F;Aljumaily, RMK;Kitchen, M;Forsyth, NR; | DNMT3B Is an Oxygen-Sensitive De Novo Methylase in Human Mesenchymal Stem Cells | Cells | 33925659 | nd Methods 2.1. Isolation and Culture of BM-hMSCs Three human BM-hMSCs BMA-16 (Male, Age—29 years, Lonza, MD, USA), BMA-20 (Female, Age—50 years, Lonza, MD, USA) and BMA-25 (Male, Age—27 years, AllCells, LLC, Alameda, CA, USA) were isolated from comm | Full Paper |
2021 | Miao, G;Sun, X; | Development of a novel anti-B7-H4 antibody enhances anti-tumor immune response of human T cells | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | 34328096 | Human PBMCs were purchased from ALLCELLS. Hybridoma cell lines were cultured in H-SFM complete medium. CD8+ T cells were cultured in RPMI 1640 medium containing 2 mM L- Save | Full Paper |
2021 | Gu, C;Cao, X;Wang, Z;Hu, X;Yao, Y;Zhou, Y;Liu, P;Liu, X;Gao, G;Hu, X;Zhang, Y;Chen, Z;Gao, L;Peng, Y;Jia, F;Shan, C;Yu, L;Liu, K;Li, N;Guo, W;Jiang, G;Min, J;Zhang, J;Yang, L;Shi, M;Hou, T;Li, Y;Liang, W;Lu, G;Yang, C;Wang, Y;Xia, K;Xiao, Z;Xue, J;Huang, X;Chen, X;Ma, H;Song, D;Pan, Z;Wang, X;Guo, H;Liang, H;Yuan, Z;Guan, W;Deng, SJ; | A human antibody of potent efficacy against SARS-CoV-2 in rhesus macaques showed strong blocking activity to B.1.351 | mAbs | 34097570 | ed SARS-CoV-2 RBD (Sino Biological, 40592-V05H). Phage libraries were constructed using antibody gene fragments amplified from peripheral blood mononuclear cells (PBMCs) of 50 healthy human subjects (Allcells, PB003F and PB003 C) and total RNA from P | Full Paper |
2021 | Chen, J;Ding, J;Wu, Y;Zhang, S;Zheng, N;Yang, J;Xu, J; | Chromium Oxide Nanoparticle Impaired Osteogenesis and Cellular Response to Mechanical Stimulus | International journal of nanomedicine | 34511912 | Human MSCs were isolated from bone marrow aspirate (ALLCELLS, Lot No: BM2893). The cells were grown in α-MEM (Gibco, Thermo Fisher Scientific, UK) supplemented with 10% Save | Full Paper |
2021 | Shin, Y;Park, CM;Kim, HG;Kim, DE;Choi, MS;Kim, JA;Choi, BS;Yoon, CH; | Identification of Aristolactam Derivatives That Act as Inhibitors of Human Immunodeficiency Virus Type 1 Infection and Replication by Targeting Tat-Mediated Viral Transcription | Virologica Sinica | 32779073 | Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Alameda, CA, USA) and cultured, as described previously (Yoon et al. 2015). HIV-1 clones pNL4-3 and AD8, as well as TZM-bl and A3.01 cells, were obtained from the National Insti | Full Paper |
2021 | Wang, Y;Ni, H;Zhou, S;He, K;Gao, Y;Wu, W;Wu, M;Wu, Z;Qiu, X;Zhou, Y;Chen, B;Pan, D;Huang, C;Li, M;Bian, Y;Yang, M;Miao, L;Liu, J; | Tumor-selective blockade of CD47 signaling with a CD47/PD-L1 bispecific antibody for enhanced anti-tumor activity and limited toxicity | Cancer immunology, immunotherapy : CII | 32761423 | CD4+ T cells were isolated from human PBMC (AllCells) using EasySep human CD4+ T cells enrichment kit (StemCell Technologies). DCs were generated by incubating PBMC first Save | Full Paper |
2021 | Luban, J;Sattler, RA;Mühlberger, E;Graci, JD;Cao, L;Weetall, M;Trotta, C;Colacino, JM;Bavari, S;Strambio-De-Castillia, C;Suder, EL;Wang, Y;Soloveva, V;Cintron-Lue, K;Naryshkin, NA;Pykett, M;Welch, EM;O'Keefe, K;Kong, R;Goodwin, E;Jacobson, A;Paessler, S;Peltz, SW; | The DHODH inhibitor PTC299 arrests SARS-CoV-2 replication and suppresses induction of inflammatory cytokines | Virus research | 33249060 | c. Uridine, brequinar, and A77 were obtained from Sigma-Aldrich (St. Louis, MO). Anti IL-4 and anti-IFL-γ antibodies were obtained from BD Biosciences (San Jose, CA). Frozen PBMCs were obtained from AllCells (Alameda, CA). IL-17, IL-17 F and IL-23 EL | Full Paper |
2021 | Satani, N;Zhang, X;Giridhar, K;Wewior, N;Cai, C;Aronowski, J;Savitz, SI; | A Combination of Atorvastatin and Aspirin Enhances the Pro-Regenerative Interactions of Marrow Stromal Cells and Stroke-Derived Monocytes In Vitro | Frontiers in pharmacology | 33959001 | d of stroke patients. Methods Isolation and Culture of Human Mesenchymal Stromal Cells (MSCs) MSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old male (AllCells, Alameda, CA) using density centrifugation | Full Paper |
2021 | Sun, LK; | Engineering genetic thermal switches for remote control of therapeutic T cells | Thesis | Primary human CD3+ T cells were obtained from anonymous donor blood following apheresis (AllCells) and were cryopreserved until subsequent use. Two days prior to lentiviral Save | Full Paper | |
2021 | Wu, HH;Ralph, KL;Sepuldeva, E;Hansen, G;Li, H;Huang, ZF;Liu, D;Dziegelewski, M;Ahlberg, J;Frego, L;Fogal, S;van Tongeren, S;Grimaldi, C;Litzenberger, T;Presky, D;Singh, S;Brodeur, S;Kroe-Barrett, R; | An optimally designed anti-human CD40 antibody with potent B cell suppression for the treatment of autoimmune diseases | International journal of pharmaceutics | 34624444 | Effector cells were generated by stimulating human peripheral blood mononuclear cells (PBMCs) (AllCells) with 10 ng/mL human IL-2 (R&D Systems) in RPMI 1640 medium (Invitrogen) Save | Full Paper |
2021 | Xiang, H;Chan, AG;Ahene, A;Bellovin, DI;Deng, R;Hsu, AW;Jeffry, U;Palencia, S;Powers, J;Zanghi, J;Collins, H; | Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancer | mAbs | 34719330 | the full-length human FGFR2b described as Ba/F3 FGFR2b, and the effector cells were peripheral blood mononuclear cells obtained fresh from individual human donors (AllCells; Save | Full Paper |
2021 | Li, G;Guo, J;Zheng, Y;Ding, W;Han, Z;Qin, L;Mo, W;Luo, M; | CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T cells | Molecular therapy oncolytics | 34553036 | CAR-T cells were generated from commercial normal donor peripheral blood mononuclear cells (PBMCs) from AllCells. The cells were further enriched from PBMC by CD3 magnetic Save;.22-μm filter, aliquoted to 2 mL cryotubes, quick frozen on dry ice, an | Full Paper |
2021 | Chen, X;Yang, S;Li, S;Qu, Y;Wang, HY;Liu, J;Dunn, ZS;Cinay, GE;MacMullan, MA;Hu, F;Zhang, X;Wang, P; | Secretion of bispecific protein of anti-PD-1 fused with TGF-β trap enhances antitumor efficacy of CAR-T cell therapy | Molecular therapy oncolytics | 33981830 | Frozen human PBMCs were obtained from AllCells. The T cell culture medium (TCM) was composed of AIM-V medium with 5% (v/v) human AB serum, 10 mM HEPES, 1% (v/v) Save;ng gag-pol. The supernatants were harvested at 48 h after transfection and filtere | Full Paper |
2021 | Branquinho, MV;Ferreira, SO;Alvites, RD;Magueta, AF;Ivanov, M;Sousa, AC;Amorim, I;Faria, F;Fernandes, MHV;Vilarinho, PM;Maurício, AC; | In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering-A Preliminary Study | International journal of molecular sciences | 34299274 | e enthalpy of fusion of 100% crystalline material [54]. (1)XC (%)=ΔHfΔHf0×100 4.3. In Vitro Assays 4.3.1. Cell Culture and Maintenance Human Dental Pulp stem/stromal cells (hDPSCs) obtained from AllCells, LLC, Alameda, CA, USA (Cat. DP0037F, Lot no. | Full Paper |
2021 | Thavapalachandran, S;Le, TYL;Romanazzo, S;Rashid, FN;Ogawa, M;Kilian, KA;Brown, P;Pouliopoulos, J;Barry, AM;Fahmy, P;Kelly, K;Kizana, E;Chong, JJH; | Pluripotent stem cell-derived mesenchymal stromal cells improve cardiac function and vascularity after myocardial infarction | Cytotherapy | 34588150 | Cells were kept cold on ice until administration to animals. BM-MSCs were purchased from AllCells (Alameda, CA, USA). Cryopreserved BM-MSCs were washed twice in DMEM and resuspended at the same density as Cymerus MSC derivatives (5 × 106 cells per 10 | Full Paper |
2021 | Kavanagh, H;Dunne, S;Martin, DS;McFadden, E;Gallagher, L;Schwaber, J;Leonard, S;O'Dea, S; | A novel non-viral delivery method that enables efficient engineering of primary human T cells for ex vivo cell therapy applications | Cytotherapy | 33941482 | cells with minimal perturbation of cell function. Methods Ethics All experimental methods were carried out in accordance with the approved guidelines. Leukopaks from healthy donors were sourced from AllCells (Quincy, MA, USA) or BioIVT (Westbury, NY | Full Paper |
2021 | Wang, Y;Zhong, K;Ke, J;Chen, X;Chen, Y;Shu, W;Chen, C;Hu, S;Sun, X;Huang, H;Luo, C;Liu, L;Yang, J;Zhang, Y;Zhi, H; | Combined 4-1BB and ICOS co-stimulation improves anti-tumor efficacy and persistence of dual anti-CD19/CD20 chimeric antigen receptor T cells | Cytotherapy | 33863641 | Human peripheral blood mononuclear cells (PBMCs) from healthy donors were obtained from AllCells. The cryopreserved PBMCs were thawed into the T-cell culture medium and Save | Full Paper |
2021 | Martin, A;Daris, M;Johnston, JA;Cui, J; | HLA-A*02:01-directed chimeric antigen receptor/forkhead box P3-engineered CD4+ T cells adopt a regulatory phenotype and suppress established graft-versus-host disease | Cytotherapy | 33309258 | A*02:01- peripheral blood mononuclear cells (PBMCs) from four donors were isolated from fresh Leukopak (AllCells) using Ficoll density gradient media. CD4+ T cells or Tregs were Save | Full Paper |
2021 | Story, JY;Zoine, JT;Burnham, RE;Hamilton, JAG;Spencer, HT;Doering, CB;Raikar, SS; | Bortezomib enhances cytotoxicity of ex vivo-expanded gamma delta T cells against acute myeloid leukemia and T-cell acute lymphoblastic leukemia | Cytotherapy | 33168453 | PBMCs (50-100 million per donor) from six different healthy donors were obtained through AllCells ( Eight total expansions from PBMCs from AllCells were performed, with two separate Save | Full Paper |
2021 | Banerjee, A;Lu, Y;Do, K;Mize, T;Wu, X;Chen, X;Chen, J; | Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases | Frontiers in cellular neuroscience | 33897370 | ity for polarization. With whole-genome transcript profile, we also demonstrated that iMG cells were clustered closely with human microglia. Materials and Methods Materials PBMCs were purchased from AllCells, LLC (Alameda, CA, USA, #PB003F and #PB00 | Full Paper |
2021 | Stem, C;Rodman, C;Ramamurthy, RM;George, S;Meares, D;Farland, A;Atala, A;Doering, CB;Spencer, HT;Porada, CD;Almeida-Porada, G; | Investigating Optimal Autologous Cellular Platforms for Prenatal or Perinatal Factor VIII Delivery to Treat Hemophilia A | Frontiers in cell and developmental biology | 34447745 | Bone marrow mononuclear cells from two different biological donors were purchased from AllCells (Alameda, CA, United States) and their purchase approved by the Office of Human Save | Full Paper |
2021 | Han, Y;Chen, Q;Zhang, L;Dissanayaka, WL; | Indispensable Role of HIF-1α Signaling in Post-implantation Survival and Angio-/Vasculogenic Properties of SHED | Frontiers in cell and developmental biology | 34368116 | e expression of target proteins that mediate adaptive metabolic mechanisms in both in vivo and in vitro survival and angiogenesis in SHED. Materials and Methods Cell Culture SHED were purchased from AllCells (Alameda, CA, United States) and cultured | Full Paper |
2021 | Yazawa, T;Inaba, H;Imamichi, Y;Sekiguchi, T;Uwada, J;Islam, MS;Orisaka, M;Mikami, D;Ida, T;Sato, T;Miyashiro, Y;Takahashi, S;Khan, MRI;Suzuki, N;Umezawa, A;Kitano, T; | Profiles of 5α-Reduced Androgens in Humans and Eels: 5α-Dihydrotestosterone and 11-Ketodihydrotestosterone Are Active Androgens Produced in Eel Gonads | Frontiers in endocrinology | 33833737 | healthy women volunteers (obtaining informed consent) at University of Fukui Hospital in 2007. Plasma was separated by centrifugation at 1000g for 5 minutes. Other plasma samples were purchased from AllCells (Alameda, CA, USA) and ProMedDX (Norton, M | Full Paper |
2021 | Sternjak, A;Lee, F;Thomas, O;Balazs, M;Wahl, J;Lorenczewski, G;Ullrich, I;Muenz, M;Rattel, B;Bailis, JM;Friedrich, M; | Preclinical Assessment of AMG 596, a Bispecific T-cell Engager (BiTE) Immunotherapy Targeting the Tumor-specific Antigen EGFRvIII | Molecular cancer therapeutics | 33632870 | Human pan-T cells (AllCells) were added at an E:T ratio of 10:1 with a dose range of AMG 596. Microvesicles were added simultaneously with T cells and AMG 596 to maintain Save | Full Paper |
2021 | Massimino, M;Tirrò, E;Stella, S;Manzella, L;Pennisi, MS;Romano, C;Vitale, SR;Puma, A;Tomarchio, C;Di Gregorio, S;Antolino, A;Di Raimondo, F;Vigneri, P; | Impact of the Breakpoint Region on the Leukemogenic Potential and the TKI Responsiveness of Atypical BCR-ABL1 Transcripts | Frontiers in pharmacology | 34276365 | CD34-positive cells derived from healthy donors were obtained from ALLCELLS. CD34-positive cells were maintained in Stem Span SFEM supplemented with cytokines at low Save | Full Paper |
2021 | Tang, J;Zou, Y;Li, L;Lu, F;Xu, H;Ren, P;Bai, F;Niedermann, G;Zhu, X; | BAY 60-6583 Enhances the Antitumor Function of Chimeric Antigen Receptor-Modified T Cells Independent of the Adenosine A2b Receptor | Frontiers in pharmacology | 33776765 | ed States) + 10% FBS (10099141C, ThermoFisher Scientific) were used to expand and culture CAR T cells. Frozen human peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from AllCells (PB005F, Alameda, CA, United States). Aft | Full Paper |
2021 | Sim, DS;Mallari, CR;Teare, JM;Feldman, RI;Bauzon, M;Hermiston, TW; | In vitro characterization of CT-001-a short-acting factor VIIa with enhanced prohemostatic activity | Research and practice in thrombosis and haemostasis | 34263099 | e binding of FVIIa to activated platelet surfaces was detected by Alexa488‐anti‐FVII (clone AA3). Donor informed consent and collection protocols are approved by the Institutional Review Board of AllCells. 2.4 Inhibition by antithrombin and tissue f | Full Paper |
2021 | Xu, Y;Barauskas, O;Kim, C;Babusis, D;Murakami, E;Kornyeyev, D;Lee, G;Stepan, G;Perron, M;Bannister, R;Schultz, BE;Sakowicz, R;Porter, D;Cihlar, T;Feng, JY; | Off-Target In Vitro Profiling Demonstrates that Remdesivir Is a Highly Selective Antiviral Agent | Antimicrobial agents and chemotherapy | 33229429 | Normal human primary bone marrow (BM) light-density cells were from three different lots obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD). All primary human cells Save | Full Paper |
2021 | Huang, Y;Jia, F;Zhao, J;Hou, Y;Hu, SQ; | Novel ACE Inhibitory Peptides Derived from Yeast Hydrolysates: Screening, Inhibition Mechanisms and Effects on HUVECs | Journal of agricultural and food chemistry | 33593053 | Primary HUVECs, as well as the endothelial growth medium (EGM) and endothelial basal serum-free medium, were purchased from Allcells Co., Ltd. (Shanghai, China). Penicillin/ Save | Full Paper |
2021 | Jing, L;Fan, S;Yao, X;Zhang, Y; | Effects of compound stimulation of fluid shear stress plus ultrasound on stem cell proliferation and osteogenesis | Regenerative biomaterials | 34868635 | lopment kit was purchased from Beyotime Biotechnology Co., Ltd. (China). The MagneSil@ Total RNA mini isolation system was purchased from Promega (USA). Primary rat BMSCs were purchased from Shanghai AllCells Biotech Co., Ltd. (China). The polytetraf | Full Paper |
2021 | Chai, ZT;Zhang, XP;Ao, JY;Zhu, XD;Wu, MC;Lau, WY;Sun, HC;Cheng, SQ; | AXL Overexpression in Tumor-Derived Endothelial Cells Promotes Vessel Metastasis in Patients With Hepatocellular Carcinoma | Frontiers in oncology | 34123800 | Primary human umbilical vein endothelial cells (HUVECs; AllCells, Shanghai, China) were cultured in endothelial cell growth medium-2 (Lonza, Basel, Switzerland) supplemented with Save | Full Paper |
2021 | Romito, M;Juillerat, A;Kok, YL;Hildenbeutel, M;Rhiel, M;Andrieux, G;Geiger, J;Rudolph, C;Mussolino, C;Duclert, A;Metzner, KJ;Duchateau, P;Cathomen, T;Cornu, TI; | Preclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV-1 Infection | Biotechnology journal | 33103367 | and data analyzed with CRISPResso2.[49] p values were adjusted with the Benjamini & Hochberg[50] method. For OCA, 1 × 106 PBMCs (ALLCELLS, USA)/mL in X-Vivo15 medium (Lonza), supplemented with 5% human AB serum (Gemini, USA) and 20 ng mL−1 of IL-2 (M | Full Paper |
2021 | Kitanaga, Y;Yamajuku, D;Kubo, S;Nakamura, K;Maeda, M;Seki, M;Kaneko, Y;Kinugasa, F;Morokata, T;Kondo, Y;Yoshinari, H;Nakayamada, S;Sumida, T;Tanaka, Y; | Discovery of a novel Igβ and FcγRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus | International immunopharmacology | 34781122 | Human peripheral blood mononuclear cells (PBMCs; Lonza, Basel, Switzerland) and normal human peripheral blood B cells (AllCells, CA, USA) were purchased. Whole blood samples were obtained from healthy subjects and patients with SLE at the University | Full Paper |
2021 | Chen, YL;Cui, Y;Liu, X;Liu, G;Dong, X;Tang, L;Hung, Y;Wang, C;Feng, MQ; | A bispecific antibody targeting HER2 and PD-L1 inhibits tumor growth with superior efficacy | The Journal of biological chemistry | 34798072 | man IgG (H + L) was obtained from Jackson ImmunoResearch. Monocyte purification kit was obtained from Miltenyi Biotec. Ficoll gradient was obtained from GE healthcare. Human PBMCs were obtained from Allcells. Humanization of anti-PD-L1 mAb by framew | Full Paper |
2021 | Langrish, CL;Bradshaw, JM;Francesco, MR;Owens, TD;Xing, Y;Shu, J;LaStant, J;Bisconte, A;Outerbridge, C;White, SD;Hill, RJ;Brameld, KA;Goldstein, DM;Nunn, PA; | Preclinical Efficacy and Anti-Inflammatory Mechanisms of Action of the Bruton Tyrosine Kinase Inhibitor Rilzabrutinib for Immune-Mediated Disease | Journal of immunology (Baltimore, Md. : 1950) | 33674445 | Human whole blood from healthy donors was purchased from AllCells and assayed for rilzabrutinib inhibition of IgE basophil activation. Whole blood was treated with ten 3-fold dilutions Save | Full Paper |
2021 | Andrejeva, G;Capoccia, BJ;Hiebsch, RR;Donio, MJ;Darwech, IM;Puro, RJ;Pereira, DS; | Novel SIRPα Antibodies That Induce Single-Agent Phagocytosis of Tumor Cells while Preserving T Cells | Journal of immunology (Baltimore, Md. : 1950) | 33431660 | were differentiated from CD14+ monocytes purchased from Astarte Biologics or selected using Pan Monocyte Isolation Kit (Miltenyi Biotec) from PBMCs (AllCells and Hemacare). Monocytes were seeded onto 96-well, flat-bottom plates at 3 × 104 cells per w | Full Paper |
2021 | Chaerkady, R;Zhou, Y;Delmar, JA;Weng, SHS;Wang, J;Awasthi, S;Sims, D;Bowen, MA;Yu, W;Cazares, LH;Sims, GP;Hess, S; | Characterization of Citrullination Sites in Neutrophils and Mast Cells Activated by Ionomycin via Integration of Mass Spectrometry and Machine Learning | Journal of proteome research | 34008986 | For mast cell preparation, three donors were used. Briefly, leukocytes were collected from Leukopak (ALLCells) and mixed with Robo II Buffer (StemCell Technologies) at 1:4 and centrifuged at 500g for 15 mins. The pellet was resuspended in 1× Vitalyse | Full Paper |
2021 | Deng, M;Wu, D;Zhang, Y;Jin, Z;Miao, J; | MiR-29c downregulates tumor-expressed B7-H3 to mediate the antitumor NK-cell functions in ovarian cancer | Gynecologic oncology | 33875234 | The human CD56 + NK cells were obtained from ALLCELLS (Emeryville, California) and were confirmed by flow cytometric analyses. NK cells were maintained in Human NK cell Save | Full Paper |
2021 | Friedman, D;Dozor, AJ;Milner, J;D'Souza, M;Talano, JA;Moore, TB;Shenoy, S;Shi, Q;Walters, MC;Vichinsky, E;Parsons, SK;Braniecki, S;Moorthy, CR;Ayello, J;Flower, A;Morris, E;Mahanti, H;Fabricatore, S;Klejmont, L;van de Ven, C;Baxter-Lowe, LA;Cairo, MS; | Stable to improved cardiac and pulmonary function in children with high-risk sickle cell disease following haploidentical stem cell transplantation | Bone marrow transplantation | 33958740 | Bioverativ, Sangamo, Veevo, Editas Medicine and is medical director for AllCells, Inc. Biosciences. SKP is a consultant to Seattle Genetics, also unrelated to this study. EV is a consultant for ApoPharma, on the advisory committee for Global Blood Th | Full Paper |
2021 | Song, D;Liu, X;Dong, C;Wang, Q;Sha, C;Liu, C;Ning, Z;Han, J;Liu, H;Zong, M;Zhao, Y;Li, Y;Liu, G;Shao, X;Dou, C; | Two novel human anti-CD25 antibodies with antitumor activity inversely related to their affinity and in vitro activity | Scientific reports | 34824364 | The effector cells PBMCs (PB003F-C, ALLCELLS) were added and the mixture was incubated at 37 C for 5 h. After substrate was added, OD490 value was read on the microplate Save;The effector cells PBMCs (PB003F-C, ALLCELLS) were added and the mixture | Full Paper |
2021 | Loo, J;Sicher, I;Goff, A;Kim, O;Clary, N;Alexeev, A;Sulchek, T;Zamarayeva, A;Han, S;Calero-Garcia, M; | Microfluidic transfection of mRNA into human primary lymphocytes and hematopoietic stem and progenitor cells using ultra-fast physical deformations | Scientific reports | 34725429 | Mobilized peripheral blood CD34-selected hematopoietic stem and progenitor cells (mPB CD34 + HSPCs, purchased from AllCells) were taken from liquid nitrogen freezer and placed Save | Full Paper |
2021 | Wang, X;Negrou, E;Maloney, MT;Bondar, VV;Andrews, SV;Montalban, M;Llapashtica, C;Maciuca, R;Nguyen, H;Solanoy, H;Arguello, A;Przybyla, L;Moerke, NJ;Huntwork-Rodriguez, S;Henry, AG; | Understanding LRRK2 kinase activity in preclinical models and human subjects through quantitative analysis of LRRK2 and pT73 Rab10 | Scientific reports | 34145320 | University of Dundee (https://mrcppureagents.dundee.ac.uk/). For the total Rab10 assay, recombinant Rab10 protein (LSBio, LS-G2178) and Rab8 protein (LS-G29310) were used. Human cryopreserved PBMCs (AllCells, PB005F) were thawed and cultured in RPMI | Full Paper |
2021 | Xue, X;De Leon-Tabaldo, A;Luna-Roman, R;Castro, G;Albers, M;Schoetens, F;DePrimo, S;Devineni, D;Wilde, T;Goldberg, S;Kinzel, O;Hoffmann, T;Fourie, AM;Thurmond, RL; | Preclinical and clinical characterization of the RORγt inhibitor JNJ-61803534 | Scientific reports | 34040108 | mentary materials and methods). Human nTreg suppressive assay Frozen purified human CD4+CD25+ natural Treg cells (nTreg), monocyte-derived dendritic cells (DC) and CD4+CD25- T effector cells (Teff) (Allcells, LLC, Alameda, CA) were thawed and co-cul | Full Paper |
2021 | Harris, KE;Lorentsen, KJ;Malik-Chaudhry, HK;Loughlin, K;Basappa, HM;Hartstein, S;Ahmil, G;Allen, NS;Avanzino, BC;Balasubramani, A;Boudreau, AA;Chang, K;Cuturi, MC;Davison, LM;Ho, DM;Iyer, S;Rangaswamy, US;Sankaran, P;Schellenberger, U;Buelow, R;Trinklein, ND; | A bispecific antibody agonist of the IL-2 heterodimeric receptor preferentially promotes in vivo expansion of CD8 and NK cells | Scientific reports | 34011961 | resuspended in 125 µL/well flow buffer and acquired on a BD FACSCelesta. Whole blood cytokine release assay Cytokine secretion was detected using fresh human whole blood (heparinized) obtained from AllCells. The following method was adapted from B. | Full Paper |
2021 | Tago, Y;Kobayashi, C;Ogura, M;Wada, J;Yamaguchi, S;Yamaguchi, T;Hayashi, M;Nakaishi, T;Kubo, H;Ueda, Y; | Human amnion-derived mesenchymal stem cells attenuate xenogeneic graft-versus-host disease by preventing T cell activation and proliferation | Scientific reports | 33510297 | Human BMSC from healthy donors were purchased from AllCells (CA, USA). The culture conditions of AMSC and BMSC (ie, MSC) were determined based on previous studies 43 . In Save | Full Paper |
2021 | Subhi, H;Husein, A;Mohamad, D;Nik Abdul Ghani, NR;Nurul, AA; | Chitosan-Based Accelerated Portland Cement Promotes Dentinogenic/Osteogenic Differentiation and Mineralization Activity of SHED | Polymers | 34641172 | SHED employed in this study were obtained from ALLCELLS, USA (Catalog No. DP004F; lot number SHED092211-01). Upon being received, the cells were characterized using stem Save | Full Paper |
2021 | Kim, OH;Park, JH;Son, JI;Yoon, OJ;Lee, HJ; | Bone Marrow Mesenchymal Stromal Cells on Silk Fibroin Scaffolds to Attenuate Polymicrobial Sepsis Induced by Cecal Ligation and Puncture | Polymers | 33946773 | y (FE-SEM; JSM 7500F, JEOL Ltd., Akishima, Tokyo, Japan). 2.3. MSC Preparation Human bone marrow MSCs were derived from the whole bone marrow of independent human donors (commercially available from AllCells, Alameda, CA, USA). The cells were cultur | Full Paper |
2021 | Wang, X;Sandberg, ML;Martin, AD;Negri, KR;Gabrelow, GB;Nampe, DP;Wu, ML;McElvain, ME;Toledo Warshaviak, D;Lee, WH;Oh, J;Daris, ME;Chai, F;Yao, C;Furney, J;Pigott, C;Kamb, A;Xu, H; | Potent, Selective CARs as Potential T-Cell Therapeutics for HPV-positive Cancers | Journal of immunotherapy (Hagerstown, Md. : 1997) | 34432728 | For human peripheral blood mononuclear cells (PBMCs), collection protocols and donor informed-consent were approved by an Institutional Review Board at Allcells. Allcells followed Save | Full Paper |
2021 | Martin, AD;Wang, X;Sandberg, ML;Negri, KR;Wu, ML;Toledo Warshaviak, D;Gabrelow, GB;McElvain, ME;Lee, B;Daris, ME;Xu, H;Kamb, A; | Re-examination of MAGE-A3 as a T-cell Therapeutic Target | Journal of immunotherapy (Hagerstown, Md. : 1997) | 33284140 | purchased from Allcells according to the method described by Garcia et al31 Collection protocols and donor informed-consent were approved by an institutional review board at Allcells. Save | Full Paper |
2021 | Xu, F;Liu, X;Zhang, D;Zhao, F;Fan, Z;Hu, S;Mei, S;Huang, Y;Sun, H;Wei, L;Guo, L;Wang, J;Cen, S;Liang, C;Guo, F; | The Engineered MARCH8-Resistant Vesicular Stomatitis Virus Glycoprotein Enhances Lentiviral Vector Transduction | Human gene therapy | 33678011 | Peripheral blood mononuclear cells (PBMCs) isolated from healthy donors were purchased from AllCells (Shanghai, China), and cultured in RPMI 1640 media containing 10% FBS ( Save | Full Paper |
2021 | Chen, Z;Chen, Z;Xu, S;Zhang, Q; | LncRNA SOX2-OT/miR-30d-5p/PDK1 Regulates PD-L1 Checkpoint Through the mTOR Signaling Pathway to Promote Non-small Cell Lung Cancer Progression and Immune Escape | Frontiers in Genetics | The microporous 2-compartment co-culture system was applied for co-culture. Transfected NSCLC cells were inoculated in the upper chamber, while CD8+ T (PB009-3-C, ALLCELLS, Shanghai) cells were inoculated in the lower chamber, allowing direct contact | Full Paper | |
2021 | Raskin, S;Van Pelt, S;Toner, K;Balakrishnan, PB;Dave, H;Bollard, CM;Yvon, E; | Novel TCR-like CAR-T cells targeting an HLA∗0201-restricted SSX2 epitope display strong activity against acute myeloid leukemia | Molecular therapy. Methods & clinical development | 34729377 | Steady-state apheresis units were purchased from AllCells and peripheral blood mononuclear cells (PBMCs) were isolated using lymphoprep density gradient. Before cryopreserving Save;ng a phase I clinical trial to evaluate the safety and anti-tumor e | Full Paper |
2021 | Xue, L;Gao, X;Zhang, H;Tang, J;Wang, Q;Li, F;Li, X;Yu, X;Lu, Z;Huang, Y;Tang, R;Yang, W; | Antiangiogenic antibody BD0801 combined with immune checkpoint inhibitors achieves synergistic antitumor activity and affects the tumor microenvironment | BMC cancer | 34686154 | #0025), 1% penicillin/streptomycin (GIBCO, Invitrogen Inc., Carlsbad, CA, USA, #15140-122) and 1% ECGS (ScienCell, #1052). For HUVEC Western blot, migration inhibition, and scratch assays, HUVECs (AllCells, Alameda, CA, USA) were maintained in HUVEC | Full Paper |
2021 | Noaks, E;Peticone, C;Kotsopoulou, E;Bracewell, DG; | Enriching leukapheresis improves T cell activation and transduction efficiency during CAR T processing | Molecular therapy. Methods & clinical development | 33718517 | Peripheral blood leukapheresis products were obtained from the NHS or purchased as LeukoPaks from AllCells, Cellex, and Anthony Nolan as part of research study NREC ID 15/LO/ Save;omplex and expensive magnetic cell sorting. Materials and methods Cu | Full Paper |
2021 | Green, R;Elliott, JL;Norona, W;Go, F;Nguyen, VT;Ge, J;Short, ML;Mulero, JJ;Zhong, C; | Developmental validation of VeriFiler™ Plus PCR Amplification Kit: A 6-dye multiplex assay designed for casework samples | Forensic science international. Genetics | 33740707 | Human genomic DNA used for the degraded DNA experiments was extracted from normal peripheral blood mononuclear cells (PBMC) of a male donor (ALLCELLS, Emeryville, CA) using a QiAamp DNA Blood Maxi Kit (Qiagen) | Full Paper |
2021 | Park, S;Huang, NWY;Wong, CXY;Pan, J;Albakr, L;Gu, J;Kang, L; | Microstructured Hyaluronic Acid Hydrogel for Tooth Germ Bioengineering | Gels (Basel, Switzerland) | 34449604 | ovine serum (Thermo Fisher, Waltham, MA, USA), penicillin-Streptomycin solution with 10,000 units penicillin and 10 mg streptomycin / mL (Thermo Fisher, Waltham, MA, USA). The culture media for DPSC (AllCells, Alameda, CA, USA) were α-Minimum essenti | Full Paper |
2021 | Garbuzova-Davis, S;Boccio, KJ;Llauget, A;Shell, R;Hailu, S;Mustafa, H;Ehrhart, J;Sanberg, PR;Appel, SH;Borlongan, CV; | Beneficial Effects of Transplanted Human Bone Marrow Endothelial Progenitors on Functional and Cellular Components of Blood-Spinal Cord Barrier in ALS Mice | eNeuro | 34479980 | Cryopreserved human bone marrow CD34+ cells (hBM34+) and human bone marrow-derived endothelial progenitor cells (hBM-EPCs) were purchased from AllCells and CELPROGEN Save | Full Paper |
2021 | Chu, J;Pieles, O;Pfeifer, CG;Alt, V;Morsczeck, C;Docheva, D; | Dental follicle cell differentiation towards periodontal ligament-like tissue in a self-assembly three-dimensional organoid model | European cells & materials | 34251657 | according to AllCells company regulations, further donor information such gender and exact age was not provided to the purchaser). The PDL-hTERT immortal cell line has already Save | Full Paper |
2021 | Madanagopal, TT;Tai, YK;Lim, SH;Fong, CH;Cao, T;Rosa, V;Franco-Obregón, A; | Pulsed electromagnetic fields synergize with graphene to enhance dental pulp stem cell-derived neurogenesis by selectively targeting TRPC1 channels | European cells & materials | 33644848 | hDPSC culture The use of human cells was approved by the NUS Institutional Review Board (NUS2094/2015). hDPSC (DPF003, AllCells, Quincy, MA, USA) were cultured in basal growth medium (DMEM, Invitrogen), supplemented with 10 % FBS (Invitrogen) in the | Full Paper |
2021 | Wang, X;Martin, AD;Negri, KR;McElvain, ME;Oh, J;Wu, ML;Lee, WH;Ando, Y;Gabrelow, GB;Toledo Warshaviak, D;Sandberg, ML;Xu, H;Kamb, A; | Extensive functional comparisons between chimeric antigen receptors and T cell receptors highlight fundamental similarities | Molecular immunology | 34419823 | Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols Save | Full Paper |
2021 | Zacharia, A;Harberts, E;Valencia, SM;Myers, B;Sanders, C;Jain, A;Larson, NR;Middaugh, CR;Picking, WD;Difilippantonio, S;Kirnbauer, R;Roden, RB;Pinto, LA;Shoemaker, RH;Ernst, RK;Marshall, JD; | Optimization of RG1-VLP vaccine performance in mice with novel TLR4 agonists | Vaccine | 33309485 | cells were also cultured in the presence of BECC470 or TLR4 agonists and the resulting upregulation of surface co-stimulatory markers was measured by flow cytometry (AllCells). Save | Full Paper |
2021 | Liu, C;Yang, G;Zhou, M;Zhang, X;Wu, X;Wu, P;Gu, X;Jiang, X; | Magnesium Ammonium Phosphate Composite Cell-Laden Hydrogel Promotes Osteogenesis and Angiogenesis In Vitro | ACS omega | 33869925 | Human umbilical vein endothelial cells (HUVECs; eahy926) were purchased from AllCells and cultured in EBM. They were seeded in the upper chamber, while the lower chambers Save | Full Paper |
2021 | Jangjoo, M;Goodman, SJ;Choufani, S;Trost, B;Scherer, SW;Kelley, E;Ayub, M;Nicolson, R;Georgiades, S;Crosbie, J;Schachar, R;Anagnostou, E;Grunebaum, E;Weksberg, R; | An Epigenetically Distinct Subset of Children With Autism Spectrum Disorder Resulting From Differences in Blood Cell Composition | Frontiers in neurology | 33935932 | ed, B cell, CD4T, CD8T, monocyte, granulocytes, and NK. These blood cell types were those on which the Houseman method was originally trained, using peripheral blood leukocyte subtypes purchased from AllCells , LLC (Emeryville, CA) or sorted cells fr | Full Paper |
2021 | Zhou, N;Chen, Y;Yang, L;Xu, T;Wang, F;Chen, L;Liu, J;Liu, G; | LncRNA SNHG4 promotes malignant biological behaviors and immune escape of colorectal cancer cells by regulating the miR-144-3p/MET axis | American journal of translational research | 34786048 | The transfected CACO2 cells were co-cultured with CD4+ T cells (PB009-2-C, AllCells Biotechnology Co., Ltd., Shanghai, China), followed by the addition of PD-1 antibody (ab52587, Abcam, UK) and PD-L1 antibody (ab205921, Abcam, UK). CD4+ T cell isolat | Full Paper |
2021 | Horton, TM;Hoff, FW;van Dijk, A;Jenkins, GN;Morrison, D;Bhatla, T;Hogan, L;Romanos-Sirakis, E;Meyer, J;Carroll, WL;Qiu, Y;Wang, T;Mo, Q;Kornblau, SM; | The effects of sample handling on proteomics assessed by reverse phase protein arrays (RPPA): Functional proteomic profiling in leukemia | Journal of proteomics | 33212251 | Normalization controls were commercially available normal adult CD34+ bone marrow cells (AllCells). Peripheral blood mononuclear cells (PBMC) were isolated using Lymphoprep Save | Full Paper |
2021 | Khouri, J;Faiman, BM;Grabowski, D;Mahfouz, RZ;Khan, SN;Wei, W;Valent, J;Dean, R;Samaras, C;Jha, BK;Lazarus, H;Campagnaro, EL;Malek, E;Reed, J;Karam, MA;Hamilton, K;Fada, S;Kalaycio, M;Liu, H;Sobecks, R;Saunthararajah, Y;Chew, Y;Orloff, M;Reu, FJ; | DNA methylation inhibition in myeloma: Experience from a phase 1b study of low-dose continuous azacitidine in combination with lenalidomide and low-dose dexamethasone in relapsed or refractory multiple myeloma | Seminars in hematology | 33509443 | Normal bone marrow mononuclear cells for separation of CD138 positive and negative fractions of 3 healthy donors was obtained commercially from ALLCELLS and processed the Save | Full Paper |
2021 | Schölzel, C;Blesius, V;Ernst, G;Goesmann, A;Dominik, A; | Countering reproducibility issues in mathematical models with software engineering techniques: A case study using a one-dimensional mathematical model of the atrioventricular node | PloS one | 34280231 | This means that the release version of the AllCells model is not only executable in OpenModelica, but also in any of the over 100 tools that support the Functional Mock-up Interface (FMI Save | Full Paper |
2021 | Dulay, RM;Valdez, BC;Li, Y;Chakrabarti, S;Dhillon, B;Kalaw, SP;Reyes, RG;Cabrera, EC; | Cytotoxicity of Gymnopilus purpureosquamulosus extracts on hematologic malignant cells through activation of the SAPK/JNK signaling pathway | PloS one | 34048499 | esuspended in freezing medium (50% heat-inactivated fetal bovine serum, 40% RPMI, 10% DMSO), and cryopreserved in liquid nitrogen until used. Mononuclear cells from healthy donors were purchased from ALLCELLS LLC (Almeda, CA, USA). For extract exposu | Full Paper |
2021 | Caplan, HW;Prabhakara, KS;Toledano Furman, NE;Zorofchian, S;Martin, C;Xue, H;Olson, SD;Cox, CS; | Human-derived Treg and MSC combination therapy may augment immunosuppressive potency in vitro, but did not improve blood brain barrier integrity in an experimental rat traumatic brain injury model | PloS one | 34038436 | Bone marrow-derived MSC were isolated from a commercially available fresh human bone marrow aspirate (AllCells, Alameda, CA) and expanded following established procedures [8, Save | Full Paper |
2021 | Baik, R;Wyman, SK;Kabir, S;Corn, JE; | Genome editing to model and reverse a prevalent mutation associated with myeloproliferative neoplasms | PloS one | 33661998 | Frozen human mobilized peripheral blood CD34+ HSPCs were purchased from AllCells and thawed according to manufacturer's instructions. CD34+ cells were cultured in StemSpan Save | Full Paper |
2021 | Garbuzova-Davis, S;Boccio, KJ;Ehrhart, J;Sanberg, PR;Appel, SH;Borlongan, CV; | Detection of endothelial cell-associated human DNA reveals transplanted human bone marrow stem cell engraftment into CNS capillaries of ALS mice | Brain research bulletin | 33545308 | Cryopreserved human bone marrow CD34+ cells (hBM34+) and human bone marrow-derived endothelial progenitor cells (hBM-EPCs) were purchased from AllCells (Alameda, CA, USA) and CELPROGEN (Torrance, CA, USA), respectively. According to the company repor | Full Paper |
2021 | Montalban-Bravo, G;Darbaniyan, F;Kanagal-Shamanna, R;Ganan-Gomez, I;Class, CA;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, KA;Chien, KS;Bueso-Ramos, C;Do, KA;Kantarjian, H;Garcia-Manero, G; | Type I interferon upregulation and deregulation of genes involved in monopoiesis in chronic myelomonocytic leukemia | Leukemia research | 33517186 | BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). Informed consent was obtained according to protocols approved by the MDACC institutional review Save | Full Paper |
2021 | Lu, J;Collins, P;Lee, KJ;Li, CM;Wang, S; | In vitro assessment of an engineered tBID-based safety switch system in human T lymphocytes | Annals of translational medicine | 34988150 | n 12-well plate and incubated the plate in incubator at 37 °C. Human T-cell samples were purchased anonymously from the vendor and considered exempt from IRB approval. The frozen human pan-T cells (AllCells, LLC., Cat# S-ISO004-06) were thawed and c | Full Paper |
2021 | Maucort, C;Di Giorgio, A;Azoulay, S;Duca, M; | Differentiation of Cancer Stem Cells by Using Synthetic Small Molecules: Toward New Therapeutic Strategies against Therapy Resistance | ChemMedChem | 32803855 | In an analogous work, it was found that ICG-001 could safely eradicate the drug-resistant CSC-like population and initiate cell differentiation in leukemia (pre-B ALLcells). The same Save | Full Paper |
2021 | Hu, J;Zhang, J;Yu, M;Liu, Z;Zou, Y;Hong, L;Zhang, T;Sun, J;Zheng, M;Zhu, X;Wang, Z;Liu, S; | Circulating miR-221/222 reduces CD4+ T cells by inhibiting CD4 expression in colorectal cancer | Acta biochimica et biophysica Sinica | 34357372 | Peripheral blood mononuclear cells (PBMCs) were purchased from ALLCELLS (PB005F; Shanghai, China). PBMCs were incubated with DynaBeads (11141D; Gibco, Carlsbad, USA), and CD3+ T cells were isolated in a magnet by binding to DynaBeads containing anti- | Full Paper |
2021 | Yang, Y;Wang, C;Dai, C;Liu, X;Li, W;Huang, M;Zhao, X;Ji, D;Li, J;Guo, W; | Amplification and expression of c-MET correlate with poor prognosis of patients with gastric cancer and upregulate the expression of PDL1 | Acta biochimica et biophysica Sinica | 33693450 | The prognostic significance of c-MET in gastric cancer (GC) remains uncertain. In the present study, we examined the amplification, expression, and the prognostic value of c-MET, Save | Full Paper |
2021 | Thorarensen, A;Balbo, P;Banker, ME;Czerwinski, RM;Kuhn, M;Maurer, TS;Telliez, JB;Vincent, F;Wittwer, AJ; | The advantages of describing covalent inhibitor in vitro potencies by IC50 at a fixed time point. IC50 determination of covalent inhibitors provides meaningful data to medicinal chemistry for SAR optimization | Bioorganic & medicinal chemistry | 33285410 | Cryopreserved human PBMCs (Catalog No. PB005F), which were used in the IL-2, IL-10, IL-15, and IFNα assays, were purchased from Allcells (Emeryville, CA). Frozen PBMCs were thawed in a water bath (37 °C), and washed once with RPMI1640 medium (Catalog | Full Paper |
2021 | Jiang, J;Li, X;Mao, F;Wu, X;Chen, Y; | Small molecular fluorescence dyes for immuno cell analysis | Analytical biochemistry | 33306976 | Statement of Ethical Use of Human Samples: All human PBMCs used in this study were obtained from AllCells Alameda, 1301 Harbor Bay Parkway, Suite 200, Alameda, CA 94502, Save;h as leukemia; and provide a quick and efficient way to study the immune | Full Paper |
2021 | Takahashi, N;Higa, A;Hiyama, G;Tamura, H;Hoshi, H;Dobashi, Y;Katahira, K;Ishihara, H;Takagi, K;Goda, K;Okabe, N;Muto, S;Suzuki, H;Shimomura, K;Watanabe, S;Takagi, M; | Construction of in vitro patient-derived tumor models to evaluate anticancer agents and cancer immunotherapy | Oncology letters | 33841567 | patient, were used in this study. Patient-derived hematopoietic tumor cells were provided by Fukushima Medical University Hospital, the Japanese Pediatric Cancer Group ALL-R14 study, ProteoGenex, or AllCells. Peripheral blood mononuclear cells (PBMCs | Full Paper |
2021 | Yoshida, K;Takabayashi, T;Imoto, Y;Sakashita, M;Kato, Y;Narita, N;Fujieda, S; | Increased Thrombin-Activatable Fibrinolysis Inhibitor in Response to Sublingual Immunotherapy for Allergic Rhinitis | The Laryngoscope | 33844301 | Systems, Minneapolis, Minnesota ), 100 ng IL-13 (R&D Systems), 100 ng IL-4 plus 1 μg/mL TAFI (Abcam, Cambridge, UK), and 100 ng IL-13 with 1 μg/mL TAFI. Human mast cells were differentiated from CD34+ hematopoietic progenitor cells (ALLCELLS, Alamed | Full Paper |
2021 | Hashim, SNM;Yusof, MFH;Zahari, W;Noordin, KBAA;Akamatsu, T;Azlina, A; | Amniotic membrane matrix effects on calcineurin-NFAT-related gene expressions of SHED treated with VEGF for endothelial differentiation | In vitro cellular & developmental biology. Animal | 34021476 | SHED (AllCells, Alameda, California) were cultured in a complete medium made of the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, Waltham, Massachusetts), supplemented with 15% of fetal bovine serum (FBS) (Gibco, Waltham, Massachusetts) | Full Paper |
2021 | Pieles, O;Hartmann, M;Morsczeck, C; | AMP-activated protein kinase and the down-stream activated process of autophagy regulate the osteogenic differentiation of human dental follicle cells | Archives of oral biology | 33254047 | 2.1. Cell culture Human dental follicle cells were purchased from AllCells and cultivated in DMEM (Dulbecco’s Modified Eagle’s Medium) high glucose with 10 % FBS (fetal bovine serum) and 1% penicillin (10,000 units/mL) / streptomycin (10 mg/mL) solut | Full Paper |
2021 | Wang, Z;Jiang, L;Wang, J;Chai, Z;Xiong, W; | Morphine promotes angiogenesis by activating PI3K/Akt/HIF-1α pathway and upregulating VEGF in hepatocellular carcinoma | Journal of gastrointestinal oncology | 34532126 | Academy of Science (Shanghai, China). The cells were cultured in 10% FBS DMEM, 37 °C, 5% CO2 incubator. HUVECs were obtained from the Allcells Company(Shanghai, China) and cultured in 10% FBS Endothelial Cell Growth Medium (ECM, Lonza, Switzerland), | Full Paper |
2021 | Hayashi, Y;Kawaki, H;Hori, M;Shintani, K;Hasegawa, T;Tanaka, M;Kondoh, N;Yoshida, T;Kawano, S;Tamaki, Y; | Evaluation of the mechanical properties and biocompatibility of gypsum-containing calcium silicate cements | Dental materials journal | 33642445 | Evaluation of the cytotoxicity of cement-immersed media Samples of hDPSCs (Allcells, Emeryville, CA, USA), which had been subcultured 11-14 times in 10% fetal bovine serum (FBS)-containing α-MEM, were seeded within a 96-well plate at a density of 4,0 | Full Paper |
2021 | Iwasa, M;Fujii, S;Fujishiro, A;Maekawa, T;Andoh, A;Takaori-Kondo, A;Ichinohe, T;Miura, Y; | Impact of 2 Gy γ-irradiation on the hallmark characteristics of human bone marrow-derived MSCs | International journal of hematology | 33386593 | Bone marrow of multiple healthy adults aged 21-44 years (#4362, #4615, and #4641) was purchased from AllCells (Emeryville, CA). Human BM-MSCs were isolated from bone marrow according to our previously published methods [12, 13]. In brief, a single-ce | Full Paper |
2021 | Cui, L;Yin, F;Cheng, J;Liu, H;Zheng, M;Liu, D;Wu, Z;Qian, Q; | Optimized cytotoxicity assay for co-suspended effector and target cells | Journal of immunological methods | 34270976 | Peripheral blood mononuclear cells (PBMCs) obtained from whole blood of healthy volunteers (AllCells, Silicon Valley, CA, USA) were isolated by density gradient centrifugation with Save | Full Paper |
2021 | Sunderic, K;Li, C;Ahmed, AHR;Dawkins, D;Azar, T;Cardoso, L;Wang, S; | Tuning Thermal Dosage to Facilitate Mesenchymal Stem Cell Osteogenesis in Pro-Inflammatory Environment | Journal of biomechanical engineering | 32601701 | Human bone marrow explanted from the iliac crest of donors was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the RosetteSep Save | Full Paper |
2021 | Koh, H;Kwon, SY;Zhen, X;Ha, HY;Lee, JH; | Generation of induced pluripotent stem cell line (KRIBBi004-A) from adult bone marrow CD34+ cells from a patient carrying 46,XX,t(1;5)(p31.1;35.1) karyotype | Stem cell research | 34736040 | Cell Source iPSC was derived from CD34+ bone marrow (BM) cells, obtained from AllCells, LLC Clonality Clonal Associated disease N/A Gene/locus Chromosome translocation t(1;5)(p31.1;35.1) Date archived/stock date Jun.2021 Cell line repository/bank The | Full Paper |
2021 | Kim, J;Koh, H;Zhen, X;Lee, DS;Ha, HY;Lee, JH; | Establishment of iPSC (KRIBBi001-A) from CD34+ group O D-negative bone marrow blood | Stem cell research | 33529979 | Cell Source iPSC was derived from CD34+ bone marrow (BM) cells, obtained from AllCells, LLC Clonality Clonal Method of reprogramming Integration-free Sendai virus-based vectors (CytoTune™-iPS 2.0 Sendai Reprogramming Kit, Life Technologies) | Full Paper |
2021 | Fu, X;Shen, X;Yin, X;Zhang, Y;Wang, X;Han, Z;Lin, Q;Fan, J; | Antioxidant and pro-apoptosis activities of coffee husk (Coffea arabica) anthocyanins | International Food Research Journal | The HUVEC and Caco-2 cell lines were purchased from ALLCELLS Biotechnology Company (Shanghai, China) and Kunming Institute of Zoology Cell Line Bank (Yunnan, China), respectively. Dulbecco’s Modified Eagle’s Medium (DMEM), foetal bovine serum, penici | Full Paper | |
2021 | Choi, J;Goulding, SP;Conn, BP;McGann, CD;Dietze, JL;Kohler, J;Lenkala, D;Boudot, A;Rothenberg, DA;Turcott, PJ;Srouji, JR;Foley, KC;Rooney, MS;van Buuren, MM;Gaynor, RB;Abelin, JG;Addona, TA;Juneja, VR; | Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutations | Cell reports methods | 35474673 | (HLA-A∗03:01, HLA-A∗11:01, HLA-A∗68:01, HLA-B∗07:02, HLA-C∗01:02, HLA-C∗03:04, and/or HLA-C∗08:02) were isolated using Ficoll separation from apheresis material (AllCells Save;130-050-201; RRID:AB_2665482 CD25 MicroBeads, human Miltenyi Biotec | Full Paper |
2021 | Cai, W;Dong, J;Gallolu Kankanamalage, S;Titong, A;Shi, J;Jia, Z;Wang, B;Huang, C;Zhang, J;Lin, J;Kan, SZ;Han, S;Zhou, J;Liu, Y; | Biological activity validation of a computationally designed Rituximab/CD3 T cell engager targeting CD20+ cancers with multiple mechanisms of action | Antibody therapeutics | 34805746 | They were cultured in RPMI 1640 (Thermo fisher) medium supplemented with 10% fetal bovine serum (FBS) (GIBCO) at 37 °C, 5% CO2. Human peripheral blood mononuclear cells (hPBMC) were purchased from AllCells (Alameda, CA) and HumanCells Bio (San Jose, | Full Paper |
2021 | Moe, GR;Steirer, LM;Lee, JA;Shivakumar, A;Bolanos, AD; | A cancer-unique glycan: de-N-acetyl polysialic acid (dPSA) linked to cell surface nucleolin depends on re-expression of the fetal polysialyltransferase ST8SIA2 gene | Journal of experimental & clinical cancer research : CR | 34544457 | rting (FACS) binding, and fluorescence labeling experiments were treated with Accutase and washed with medium before use. Human normal peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Alameda, CA). Protein extraction from ce | Full Paper |
2021 | Xing, Y;Chu, KA;Wadhwa, J;Chen, W;Zhu, J;Bradshaw, JM;Shu, J;Foulke, MC;Loewenstein, N;Nunn, P;By, K;Phiasivongsa, P;Goldstein, DM;Langrish, CL; | Preclinical Mechanisms of Topical PRN473, a Bruton Tyrosine Kinase Inhibitor, in Immune-Mediated Skin Disease Models | ImmunoHorizons | 34326199 | Human whole blood (AllCells) pretreated for 1 h with eleven 3-fold serial dilutions of PRN473, starting at a concentration of 10 μM, was incubated overnight with goat anti-human IgM F( Save | Full Paper |
2021 | Pohlmeyer, CW;Shang, C;Han, P;Cui, ZH;Jones, RM;Clarke, AS;Murray, BP;Lopez, DA;Newstrom, DW;Inzunza, MD;Matzkies, FG;Currie, KS;Di Paolo, JA; | Characterization of the mechanism of action of lanraplenib, a novel spleen tyrosine kinase inhibitor, in models of lupus nephritis | BMC rheumatology | 33781343 | healthy donors or individuals with SLE (AllCells) were treated with lanraplenib or dimethyl Naive B cells were isolated from freshly collected PBMCs (AllCells) by negative selection ( Save | Full Paper |
2021 | Nordin, NS;Mokhtar, KI;Mustafa, BE; | Effects of Flaxseed (Linum usitatissimum) Extract on the Osteoblast Differentiation Potential of Stem Cells Derived from Human Exfoliated Deciduous Teeth | Makara Journal of Health Research | SHED (ALLCells, USA) were cultured in complete growth media (alpha-MEM; 10% FBS; 0.5% Pen-Strep) until confluence. SHED at 80%-90% confluence were subjected to osteoblast differentiation. The complete growth medium was replaced with osteoblast induct | Full Paper | |
2021 | Turkson, A;Addor, J;Kharib, D; | Validating Intrinsic Factors Informing E-Commerce: Categorical Data Analysis Demo | Open Journal of Statistics | ows a chi-square distribution, and the conclusion depends on whether or not the obtained statistic is greater that the critical statistic at a chosen alpha level. The test statistics is: χ 2 = ∑ allcells ( Observed − Expected ) 2 Expected = ∑ i , j | Full Paper | |
2021 | Xu, Y;Shen, L;Li, G; | The The Effect of Elution Volume for Immunoprecipitation on m6A-Seq Analysis | Proceedings of Anticancer Research | The Mouse Renal Carcinoma cells (RenCa) were cultured in DMEM medium (Invitrogen) containing 10% FBS, 2 mM L-Glutamine and 1% penicillin-streptomycin. RenCa cell line was purchased from Allcells Biotechnology (Shanghai, China) and were routinely test | Full Paper | |
2021 | Zhou, Z;Zheng, J;Lin, D;Chen, Y;Hu, X; | Exosomes Derived from Dental Pulp Stem Cells Accelerate Cutaneous wound Healing by Enhancing Angiogenesis via Cdc42/p38 MAPK Pathway | Research Square | 1% penicillin-streptomycin (Sigma, USA). HUVECs were bought from ALLCELLS (Shanghai, China) and expanded in endothelial cell growth medium (EGM). For exosome co-cultures experiment, 10μg/ml of exosomes were added to the culture medium of HUVECs. All | Full Paper | |
2021 | Blitzer, G;Paz, C;Glassey, A;Ganz, O;Giri, J;Pennati, A;Meyers, R;Lunga, T;Robbins, D;Thibeault, S;Bates, A;Nickel, K;Morris, Z;Mattison, R;Chappell, R;Glazer, T;Rogus-Pulia, N;Galipeau, J;Kimple, R; | A Pilot Study to Assess the Salivary Gland Regenerative Potential of Bone Marrow Mesenchymal Stromal Cells from Treated Head and Neck Cancer Patients | Research Square | Bone marrow from healthy adult control volunteers were purchased from a commercial source (AllCells, Alameda, CA). We had originally planned to enroll 15 patients: 5 patients with Save | Full Paper | |
2021 | Tie, B;Guo, Z;Li, L;Wang, W;Liu, R;Fang, J; | miR-3156-5p Enhances Hypoxia-Induced Angiogenesis in Hepatocellular Carcinoma via Modulating the SOCS5/HIF-1α/VEGF Pathway | Research Square | Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in the medium containing 50 U/mL penicillin and 50 U/mL streptomycin (Invitgen, San Diego, CA), 50 mL endothelial cell growth additive (Upstat | Full Paper | |
2021 | Chen, S;Barrientos, J;Ferrer, G;Ravichandran, P;Ibrahim, M;Kieso, Y;Kutok, J;Peluso, M;Sharma, S;Weaver, D;Pachter, J;Rai, K;Chiorazzi, N; | Duvelisib Eliminates CLL B Cells, Impairs CLL-Supporting Cells, and Overcomes Ibrutinib Resistance in Preclinical Models | Research Square | Human PBMCs from CLL donors obtained from whole blood from a commercial source (Bioreclamation IVT or ALLCELLS) were resuspended (1 x 106 cells/mL) in RPMI-1640 medium Save | Full Paper | |
2021 | Valadkhan, S;Plasek, L;Gunawardane, L;Niazi, F;Mason, S;Leskov, K;Perez, G;Dobrowolski, C;Shukla, M;Nutt, W;Karn, J; | An HIV-induced mechanism for T cell quiescence andproviral latency | Research Square | Peripheral blood mononuclear cells from HIV negative Caucasian male donors were purchased from Allcells. Naive CD4+ T cells were isolated using EasySep Human Naive CD4+ T Save | Full Paper | |
2021 | Cai, W;Dong, J;Kankanamalage, S;Titong, A;Shi, J;Jia, Z;Wang, B;Huang, C;Zhang, J;Lin, J;Kan, S;Zhou, J;Liu, Y; | A computationally designed Rituximab/CD3 T cell engager targeting CD20+ cancers with multiple mechanisms of action | Research Square | medium supplemented with 10% fetal bovine serum (FBS) (GIBCO) 115 at 37°C, 5% CO2. Human peripheral blood mononuclear cells (hPBMC) were purchased from 116 AllCells (Alameda, CA) and HumanCells Bio (San Jose, CA). Rituximab resistant Raji cells 117 ( | Full Paper | |
2021 | Zhao, C;Zhang, W;Gong, G;Xie, L;Wang, M;Hu, Y; | A New Approach to Produce IgG4-like Bispecific Antibodies | Research Square | Human CD4+ T cells were purified from PBMC (AllCells, China) by negative selection with CD4+ T cell enrichment cocktail kit (Stemcell, Vancouver, Canada) according to the manufacturer’s instruction. Immature DC (iDC) was generated from monocytes by c | Full Paper | |
2021 | Noto, P; | Analysis of Liver X Receptor target gene expression across species | Drexel University | cDNA from Human MTC™ Panels I and II were purchased from Clontech (catalog# 636742 and 636743) and subjected to real-time PCR. Whole blood from human donors was purchased from AllCells (catalog# WB001) | Full Paper | |
2021 | Dunbar, A;Kim, D;Lu, M;Farina, M;Yang, J;Park, Y;Gobbo, F;Verachi, P;Martelli, F;Karzai, A;Xiao, W;Xia, L;Elmansy, N;Kleppe, M;Chen, Z;Xiao, Y;McGovern, E;Snyder, J;Krishnan, A;Hill, C;Cordner, K;Zouak, A;Salama, M;Yohai, J;Tucker, E;Chen, J;Zhou, J;McConnell, T;Koche, R;Rampal, R;Migliaccio, A;Fan, R;Levine, R;Hoffman, R; | CXCL8/CXCR2 signaling mediates bone marrow fibrosis and represents a therapeutic target in myelofibrosis | bioRxiv | Pro-inflammatory signaling is a hallmark feature of human cancer, including in myeloproliferative neoplasms (MPNs), most notably myelofibrosis (MF). Dysregulated inflammatory Save | Full Paper | |
2021 | Kartha, V;Duarte, F;Hu, Y;Ma, S;Chew, J;Lareau, C;Earl, A;Burkett, Z;Kohlway, A;Lebofsky, R;Buenrostro, J; | Functional Inference of Gene Regulation using Single-Cell Multi-Omics | bioRxiv | CD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from AllCells (see Table S1 for catalog numbers and donor information). Cells were quickly thawed in a 37°C water bath, rinsed with culture medium (Iscove’s Modified | Full Paper | |
2021 | Kong, T;Laranjeira, A;Yang, K;Fisher, D;Yu, L;Wang, A;Ruzinova, M;Fowles, J;Allen, M;Celik, H;Challen, G;Huang, S;Oh, S; | DUSP6 mediates resistance to JAK2 inhibition and drives leukemic progression | bioRxiv | gradient extraction and cryopreserved according to standard procedures. Additional BMMCs were purchased from AllCells (Alameda, California). A list of patient samples utilized in this study is provided in Table 1 | Full Paper | |
2021 | Qi, J;D’Souza, D;Dawson, T;Geanon, D;Stefanos, H;Marvin, R;Walker, L;Rahman, A; | Multimodal Single-Cell Characterization of the Human Granulocyte Lineage | bioRxiv | Fresh whole bone marrow was purchased from AllCells Inc. and delivered on the day of the experiment. The bone marrow was in a volume of 3mL and the sample was limited to 2 units/ Save | Full Paper | |
2021 | Kim, J;Schulte, A;Sarver, A;Angelos, M;Frantz, A;Forster, C;O’Brien, T;Cornax, I;O’Sullivan, M;Cheng, N;Lewellen, M;Oseth, L;Kumar, S;Bullman, S;Pedamallu, C;Goyal, S;Meyerson, M;Lund, T;Alfoldi, J;Breen, M;Lindblad-Toh, K;Dickerson, E;Kaufman, D;Modiano, J; | Transcriptional and Functional Activity of Hemangiosarcoma Support Bone Marrow Nurse Cell Ontogeny | bioRxiv | Human BM-derived mesenchymal stromal cells (hBM-MSCs) were isolated from whole bone marrow purchased from AllCells (Emeryville, CA, USA) as previously described [24-27]. M2- Save | Full Paper | |
2021 | Maughon, T;Shen, X;Huang, D;Michael, A;Shockey, W;Andrews, S;McRae, J;Platt, M;Fernández, F;Edison, A;Stice, S;Marklein, R; | Multi-omics characterization of mesenchymal stem/stromal cells for the identification of putative critical quality attributes | bioRxiv | PBMCs (AllCells, Alameda CA) were thawed in RPMI media (RPMI, 20% FBS, 2mM L-glutamine, 50 U/mL penicillin, 50 μg/mL streptomycin) and cultured overnight at 37°C and 5% CO2. Prior to co-culture, PBMCs were labeled with CFSE (Supplemental Table 1, Bio | Full Paper | |
2021 | Jain, S;Rick, J;Joshi, R;Beniwal, A;Spatz, J;Chang, A;Nguyen, A;Sudhir, S;Chandra, A;Haddad, A;Wadhwa, H;Shah, S;Choi, S;Hayes, J;Wang, L;Yagnik, G;Costello, J;Diaz, A;Aghi, M; | Identification of Cancer-Associated Fibroblasts in Glioblastoma and Defining Their Pro-tumoral Effects | bioRxiv | THP-1 cells and monocytes isolated from peripheral blood (AllCells) run through the MojoSort Human CD14 Selection Kit were treated with 50 ng/μL PMA (phorbol myristate acetate) Save | Full Paper | |
2021 | Andrews, S;Klinker, M;Bauer, S;Marklein, R; | Morphological Landscapes from High Content Imaging Identify Optimal Priming Strategies that Enhance MSC Immunosuppression | bioRxiv | Human bone marrow-derived MSCs were obtained from 4 different donors purchased from Lonza (Walkersville, MD, USA), AllCells (Emeryville, CA, USA), and RoosterBio (Frederick, MD, USA) (see Table S1 for donor information). MSC culture conditions for th | Full Paper | |
2021 | Gu, C;Cao, X;Wang, Z;Hu, X;Yao, Y;Zhou, Y;Liu, P;Liu, X;Gao, G;Hu, X;Zhang, Y;Chen, Z;Gao, L;Peng, Y;Jia, F;Shan, C;Yu, L;Liu, K;Li, N;Guo, W;Jiang, G;Min, J;Zhang, J;Yang, L;Shi, M;Hou, T;Li, Y;Liang, W;Lu, G;Yang, C;Wang, Y;Xia, K;Xiao, Z;Xue, J;Huang, X;Chen, X;Ma, H;Song, D;Pan, Z;Wang, X;Guo, H;Liang, H;Yuan, Z;Guan, W;Deng, S; | A human antibody with blocking activity to RBD proteins of multiple SARS-CoV-2 variants including B.1.351 showed potent prophylactic and therapeutic efficacy against SARS-CoV-2 in rhesus macaques | bioRxiv | Phage libraries were constructed using antibody gene fragments amplified from PBMCs of 50 healthy human subjects (Allcells, PB003F and PB003C) and total RNA from PBMCs ( Save | Full Paper | |
2021 | Fitzgerald, A;Marcisak, E;Nasir, A;Glasgow, E;Jablonski, S;Van Der Veken, P;Pearson, G;Fertig, E;Mace, E;Weiner, L; | Fibroblast activation protein regulates natural killer cell migration, extravasation and tumor infiltration | bioRxiv | from AllCells with either CD56 positive selection or CD56 negative selection (Allcells, cat# T cells, B cells and monocytes were isolated from PBMCs (Allcells) using Mojosort magnetic Save | Full Paper | |
2021 | Brooks, J; | Preclinical Dynamic Contrast Enhanced Imaging for Longitudinal Biophysical Assessments of the Healthy and Malignant Vasculature after Radiotherapy | Thesis | C57BL/6J mice (Strain 000664, Jackson Laboratory, Bar Harbor, ME) mice were injected with 1x106 GFP+ BCR-ABL (p190Kd) expressing B-cell ALLcells [66], suspended in 200 µl of phosphate buffered saline, through the tail vein. To test drug delivery, inj | Full Paper | |
2021 | O'Connor, EI; | Mechanisms Behind the Causal Role of Dietary Oxylipins of the Lipoxygenase Pathway in the Development of Pulmonary Arterial Hypertension | Thesis | PAECs were incubated with or without 15-HETE and 12 hours later healthy CD8 cells (ALLCELLS, Lot# 3003028) were added. After 12 hours of contact between EC and CD8 cells, the cells were collected, fixed, and stained for cleaved caspase 3 | Full Paper | |
2021 | McIntyre, P; | The Effects of a Pyk2 Kinase Inhibitor on the Proliferation and Differentiation of Human Dental Pulp Stem Cells | Thesis | Commercially available human DPSCs, collected from the pulp of extracted teeth, have been previously purchased from ALLCells and stored in liquid nitrogen (Alameda, CA, USA). • Save | Full Paper | |
2021 | Noaks, E; | Enhancing the consistency of donor derived peripheral blood mononuclear cells for CAR T-cell manufacturing | Thesis | For work completed at the company site healthy peripheral blood leukapheresis were obtained from the NHS or purchased as LeukoPaks from AllCells, Cellex and Anthony Nolan, as part of research study NREC ID 15/LO/1322. Experiments using healthy leukap | Full Paper | |
2021 | Osman, ZF;Zahari, W;Kannan, TP; | Ultrastructure changes in BMP-2-treated SHED cultured on glycerol preserved human amniotic membrane | Acta Microscópica | ommerciallysourcedSHED(AllCells,USA)wasculturedonde-epithelialized2cmx2cmglycerol-preserved γ-radiated AM in a culture dish containing 3 mlof media at different cell density for the respective days asthefollowing;2x105forday1,5x104forday7,2.5x104 | Full Paper | |
2021 | Luecken, MD;Burkhardt, DB;Cannoodt, R; | A sandbox for prediction and integration of dna, rna, and proteins in single cells | Conference | We sourced multiple samples of BMMCs from 10 donors via AllCells (California, USA), all healthy non-smokers without recent medical treatment. Donors varied by age (22 - 40), sex, Save | Full Paper | |
2021 | Vladimirova, T; | Implementation and Evaluation of Verlet List-based Methods in AutoPas | Thesis | Inside the 2D vector for AllCells or 3D vector for CellPair respectively, the method adds the particle indices corresponding to the particle pointers from the same spot in the AoS neighbor Save | Full Paper | |
2021 | Maser, IP; | In-depth characterization of myeloid enhanced humanized mouse strains | Thesis | was diluted with 0.9% sodium chloride (Braun) and 15 mg/kg was intraperitoneally (i.p.) injected into mice. 24 hours later 1×105 human CD34+ umbilical cord blood-derived hematopoietic stem cells (HSCs) (Stem Cell Technologies/Allcells) in 100 µl phos | Full Paper | |
2021 | Fitzgerald, AA; | Enhancing Anti-Cancer Properties of Human Natural Killer Cells | Thesis | Fresh healthy donor NK cells were purchased from AllCells with either CD56 positive selection or CD56 negative selection (Allcells, cat#PB012-P or PB012-N). T cells, B cells and monocytes were isolated from PBMCs (Allcells) using Mojosort magnetic ce | Full Paper | |
2021 | Butler, A; | Computational Methods for the Integration of Single-Cell Data | Thesis | We therefore repeated the original experiment, where PBMCs from a healthy human donor (ALLCELLS) were cultured with RPMI medium supplemented with 10% FBS and stimulated for 6 h with IFNβ (100 U/ml, PBL Assay Science), with a subset of cells left unex | Full Paper | |
2021 | Nataraja, S;Singh, M;Demes, S;Olson, L;Stanwix, J;Biddle, M;Clarke, E;Huang, Y;Nguyen, J;Chen, C;Zhang, P;Abdulla, F;Vercellotti, G;Belcher, J; | ML-0207/ASP8731: A Novel BACH1 Inhibitor That Induces Fetal Hemoglobin in Treatment of Sickle Cell Disease | Blood | Translational Development, Mitobridge, Cambridge, MA++Translational Science, Mitobridge, Cambridge, MA++Clinical Pharmacology and Exploratory Development, Astellas, Northbrook, IL++Translational Science, Mitobridge, Cambridge, MA++Translational Scien | Full Paper | |
2021 | Qing, Y;Dong, L;Gao, L;Li, C;Li, Y;Han, L;Prince, E;Tan, B;Deng, X;Wetzel, C;Shen, C;Gao, M;Chen, Z;Li, W;Zhang, B;Braas, D;Ten Hoeve, J;Sanchez, GJ;Chen, H;Chan, LN;Chen, CW;Ann, D;Jiang, L;Müschen, M;Marcucci, G;Plas, DR;Li, Z;Su, R;Chen, J; | R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis | Molecular cell | 33434505 | The Lin− progenitor cells were then subject to retroviral transduction with MSCV-Neo-MA9 construct by two rounds of ‘spinoculation’, as described previously (Li et al., 2015). After being selected for 7 days with 0.5 mg/mL G418 Sulfate (10131027, Gib | Full Paper |
2021 | Li, C;Wang, H;Georgakopoulou, A;Gil, S;Yannaki, E;Lieber, A; | In Vivo HSC Gene Therapy Using a Bi-modular HDAd5/35++ Vector Cures Sickle Cell Disease in a Mouse Model | Molecular therapy : the journal of the American Society of Gene Therapy | 32949495 | The lineage-minus (Lin−) cells were isolated by depletion of lineage-committed cells in bone marrow MNCs using the Mouse Lineage Cell Depletion Kit (Miltenyi Biotec, San Diego, CA, USA) according to the manufacturer’s instructions. CFU assays were pe | Full Paper |
2021 | Loftus, JP;Yahiaoui, A;Brown, PA;Niswander, LM;Bagashev, A;Wang, M;Schauf, A;Tannheimer, S;Tasian, SK; | Combinatorial efficacy of entospletinib and chemotherapy in patient-derived xenograft models of infant acute lymphoblastic leukemia | Haematologica | 32414848 | s Supplementary Appendix Acknowledgments We acknowledge Dr Ann Forslund and Ms Chelsea Mullins formerly at Gilead Sciences for study protocol management and data analysis, and Dr Emer Clarke at ReachBio Research Labs for assistance with experim | Full Paper |
2021 | DiLillo, DJ;Olson, K;Mohrs, K;Meagher, TC;Bray, K;Sineshchekova, O;Startz, T;Kuhnert, J;Retter, MW;Godin, S;Sharma, P;Delfino, F;Lin, J;Smith, E;Thurston, G;Kirshner, JR; | A BCMAxCD3 bispecific T cell-engaging antibody demonstrates robust antitumor efficacy similar to that of anti-BCMA CAR T cells | Blood advances | 33651100 | T cells were purified from normal donor PBMCs (ReachBio) by using magnetic beads (Thermo Fisher Scientific) and activated with CD3/CD28 microbeads (Thermo Fisher Scientific) in Save | Full Paper |
2021 | Byrgazov, K;Besse, A;Kraus, M;Slipicevic, A;Lehmann, F;Driessen, C;Besse, L; | Novel Peptide-drug Conjugate Melflufen Efficiently Eradicates Bortezomib-resistant Multiple Myeloma Cells Including Tumor-initiating Myeloma Progenitor Cells | HemaSphere | 34136753 | of MM were assessed in a semisolid methylcellulose-based media formulation containing 10% phytohemagglutinin (PHA)-stimulated leukocyte conditioned medium (ReachBio, WA). Save;cell outgrowth assay Clonogenic progenitors of MM were assessed in a sem | Full Paper |
2021 | Swaminathan, M;Kopyt, N;Atta, MG;Radhakrishnan, J;Umanath, K;Nguyen, S;O'Rourke, B;Allen, A;Vaninov, N;Tilles, A;LaPointe, E;Blair, A;Gemmiti, C;Miller, B;Parekkadan, B;Barcia, RN; | Pharmacological effects of ex vivo mesenchymal stem cell immunotherapy in patients with acute kidney injury and underlying systemic inflammation | Stem cells translational medicine | 34581517 | Flow cytometry testing of patient leukocytes was performed by ReachBio (Spokane, Washington). Blood was collected in CPT tubes (362 753; BD Biosciences), and peripheral blood Save;n a plasma preparation tube (362 788; BD Biosciences, San Jose, Cali | Full Paper |
2021 | Lin, M;Kowolik, CM;Xie, J;Yadav, S;Overman, LE;Horne, DA; | Potent Anticancer Effects of Epidithiodiketopiperazine NT1721 in Cutaneous T Cell Lymphoma | Cancers | 34282785 | TCC (Manassas, VA, USA), authenticated by STR-profiling at the source and passaged for less than 6 months after receipt or resuscitation. Peripheral blood mononuclear cells (PBMCs) were obtained from ReachBio (Spokane, WA, USA). All cells were grown | Full Paper |
2021 | Samuelson, C;Radtke, S;Zhu, H;Llewellyn, M;Fields, E;Cook, S;Huang, MW;Jerome, KR;Kiem, HP;Humbert, O; | Multiplex CRISPR/Cas9 genome editing in hematopoietic stem cells for fetal hemoglobin reinduction generates chromosomal translocations | Molecular therapy. Methods & clinical development | 34853798 | cation event frequencies was also undertaken on each of these tissues from necropsy as per protocol above. CFU and HbF assays on mouse BM A total of 7 × 104/mL cells were plated in ColonyGEL 1402 (ReachBio, Seattle, WA) in triplicate from each mouse | Full Paper |
2021 | Sharifi, S;Sharifi, H;Akbari, A;Chodosh, J; | Systematic optimization of visible light-induced crosslinking conditions of gelatin methacryloyl (GelMA) | Scientific reports | 34857867 | and exposed to photoirradiation for a 4 and 10 min. Those cell-laden gels were immediately washed 3 times with PBS and incubated in 200 μL of an optimized serum-containing medium (catalog # 02,100, Reachbio lab, Spokane, WA) at 37 °C. Cell viability | Full Paper |
2021 | Haber, L;Olson, K;Kelly, MP;Crawford, A;DiLillo, DJ;Tavaré, R;Ullman, E;Mao, S;Canova, L;Sineshchekova, O;Finney, J;Pawashe, A;Patel, S;McKay, R;Rizvi, S;Damko, E;Chiu, D;Vazzana, K;Ram, P;Mohrs, K;D'Orvilliers, A;Xiao, J;Makonnen, S;Hickey, C;Arnold, C;Giurleo, J;Chen, YP;Thwaites, C;Dudgeon, D;Bray, K;Rafique, A;Huang, T;Delfino, F;Hermann, A;Kirshner, JR;Retter, MW;Babb, R;MacDonald, D;Chen, G;Olson, WC;Thurston, G;Davis, S;Lin, JC;Smith, E; | Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning | Scientific reports | 34257348 | ce with the ARRIVE guidelines. OVCAR-3 bioluminescence imaging (BLI) study Eight-week-old NOD SCID gamma chain knock-out (NSG) mice (Jackson Laboratory) were injected with 5 × 106 human PBMCs (ReachBio) before 1 × 106 ascites cells from the OVCAR-3/ | Full Paper |
2021 | Patterson, AM;Sellamuthu, R;Plett, PA;Sampson, CH;Chua, HL;Fisher, A;Vemula, S;Feng, H;Katz, BP;Tudor, G;Miller, SJ;MacVittie, TJ;Booth, C;Orschell, CM; | Establishing Pediatric Mouse Models of the Hematopoietic Acute Radiation Syndrome and the Delayed Effects of Acute Radiation Exposure | Radiation research | 33577641 | elsewhere (47). Hematopoietic progenitor cell (HPC) number and function were quantitated from 1.0 × 10e5 low-density bone marrow (LDBM) in 1 ml ColonyGEL™ Mouse Complete Medium (ReachBio Research Labs, Seattle, WA) | Full Paper |
2021 | Rethnam, M;Tan, DQ;Suda, T; | Myeloma cells self-promote migration by regulating TAB1-driven TIMP-1 expression in mesenchymal stem cells | Biochemical and biophysical research communications | 33183761 | Human BM MSCs (#02100, ReachBio) were grown in DMEM low glucose media (Biowest) supplemented with 10% FBS and 1% Antibiotic/Anti-mycotic (Gibco). Cells were passaged upon confluence and cultured at a density of 4.5 × 103 cells/cm2. U266-B1 (TIB-196, | Full Paper |
2021 | Andersen, A;Vieira-Brock, PL;Vaughan, B;Vollmer, D; | Method development for the analysis of PBMC-mediated killing of K562 cells by bovine colostrum | Journal of immunological methods | 34744024 | Declaration of Competing Interest Authors and principle investigators are employed by 4Life Research, which sells the test articles commercially. 4Life Research funded all portions of this study. Acknowledgements All testing was performed at ReachBi | Full Paper |
2021 | Radtke, S;Enstrom, M;Pande, D;Cui, M;Madhu, R;Perez, A;Kiem, H; | Hematopoietic recovery after transplantation is primarily derived from the stochastic contribution of hematopoietic stem cells | bioRxiv | For CFC assays, 800-1,200 sort-purified CD34+ cells and CD34-subpopulations were seeded into 3.5 ml ColonyGEL 1402 (ReachBio, Seattle, WA). Colonies were scored after 12 to 14 days, discriminating colony forming unit- (CFU-) granulocyte (CFU-G), macr | Full Paper | |
2020 | Geoerger, B;Kang, HJ;Yalon-Oren, M;Marshall, LV;Vezina, C;Pappo, A;Laetsch, TW;Petrilli, AS;Ebinger, M;Toporski, J;Glade-Bender, J;Nicholls, W;Fox, E;DuBois, SG;Macy, ME;Cohn, SL;Pathiraja, K;Diede, SJ;Ebbinghaus, S;Pinto, N; | Pembrolizumab in paediatric patients with advanced melanoma or a PD-L1-positive, advanced, relapsed, or refractory solid tumour or lymphoma (KEYNOTE-051): interim analysis of an open-label, single-arm, phase 1–2 trial | Lancet Oncol. | 31812554 | In all patients, baseline PD-L1 expression in tumour samples (positivity defined as any staining of the stroma or PD-L1 expression on at least 1% of tumour cells) was assessed centrally at QualTek Molecular Laboratories (Goleta, CA, USA) using a prot | Full Paper |
2020 | Emens, LA;Esteva, FJ;Beresford, M;Saura, C;De Laurentiis, M;Kim, SB;Im, SA;Wang, Y;Salgado, R;Mani, A;Shah, J;Lambertini, C;Liu, H;de Haas, SL;Patre, M;Loi, S; | Trastuzumab emtansine plus atezolizumab versus trastuzumab emtansine plus placebo in previously treated, HER2-positive advanced breast cancer (KATE2): a phase 2, multicentre, randomised, double-blind trial | The Lancet. Oncology | 33002436 | The KATE2 study is a randomised, double-blind, placebo-controlled, phase 2 trial at 68 centres from nine countries across Asia, Australia, North America, and western Europe (appendix pp 13–15). Eligible patients were adults (aged ≥18 years) with cent | Full Paper |
2020 | Powell, SF;Gold, KA;Gitau, MM;Sumey, CJ;Lohr, MM;McGraw, SC;Nowak, RK;Jensen, AW;Blanchard, MJ;Fischer, CD;Bykowski, J;Ellison, CA;Black, LJ;Thompson, PA;Callejas-Valera, JL;Lee, JH;Cohen, EEW;Spanos, WC; | Safety and Efficacy of Pembrolizumab With Chemoradiotherapy in Locally Advanced Head and Neck Squamous Cell Carcinoma: A Phase IB Study | J. Clin. Oncol. | 32479189 | Testing was performed centrally by QualTek Laboratories (Santa Barbara, CA) using the PD-L1 IHC 22C3 pharmDx (Dako North America, Carpinteria, CA) IHC assay as previously described. 25 PD-L1 evaluation was performed using the combined proportional sc | Full Paper |
2020 | Marabelle, A;Cassier, P;Fakih, M;Kao, S;Nielsen, D;Italiano, A;Guren, T;Dongen, M;Spencer, K;Bariani, G;Ascierto, P;Santoro, A;Hiret, S;Ott, P;Piha-Paul, S;Liu, C;Leiby, M;Norwood, K;Delord, J; | Pembrolizumab for previously treated advanced anal squamous cell carcinoma: Pooled results from the KEYNOTE-028 and KEYNOTE-158 studies. | JCO | PD-L1 positivity). Baseline PD-L1 expression was assessed using a prototype IHC assay (QualTek) in KEYNOTE-028 and the PD-L1 IHC 22C3 pharmDx assay (Agilent Technologies) in KEYNOTE-158. Pts received pembro | Full Paper | |
2020 | Greenstein, A;Munster, P;Sachdev, J;Fleming, G;Grauer, A;Shepherd, S; | Impact of relacorilant, a selective glucocorticoid receptor antagonist, on the immunosuppressive effects of endogenous cortisol. | JCO | Tina K. Schlafly of Corcept Therapeutics. Funding for editorial, design, and production support for this poster was provided by Corcept to MedVal Scientific Information Services, LLC, Princeton, NJ. Experiments were conducted at Ardigen SA, QualTek M | Full Paper | |
2020 | Dudek, AZ;Liu, LC;Gupta, S;Logan, TF;Singer, EA;Joshi, M;Zakharia, YN;Lang, JM;Schwarz, JK;Al-Janadi, A;Alva, AS; | Phase Ib/II Clinical Trial of Pembrolizumab With Bevacizumab for Metastatic Renal Cell Carcinoma: BTCRC-GU14-003 | J. Clin. Oncol. | 32097091 | Events (CTCAE; version 4.0). Exploratory Studies. Expression of PD-L1 in archived diagnostic tumor tissue was determined by immunohistochemistry (IHC) using a 22C3 antibody (Qualtek Electronics, Newton, PA). Both a modified ;logy Criteria for Ad | Full Paper |
2020 | Tolaney, SM;Barroso-Sousa, R;Keenan, T;Li, T;Trippa, L;Vaz-Luis, I;Wulf, G;Spring, L;Sinclair, NF;Andrews, C;Pittenger, J;Richardson, ET;Dillon, D;Lin, NU;Overmoyer, B;Partridge, AH;Van Allen, E;Mittendorf, EA;Winer, EP;Krop, IE; | Effect of Eribulin With or Without Pembrolizumab on Progression-Free Survival for Patients With Hormone Receptor-Positive, ERBB2-Negative Metastatic Breast Cancer: A Randomized Clinical Trial | JAMA oncology | 32880602 | A total of 65 patients (74%) had PD-L1 testing assessed centrally by QualTek using the 22C3 antibody, and results were reported as a modified pro- portion score (MPS), defined as the proportion of cells, includ- ing both tumor and mononuclear inflamm | Full Paper |
2020 | Zsiros, E;Lynam, S;Attwood, KM;Wang, C;Chilakapati, S;Gomez, EC;Liu, S;Akers, S;Lele, S;Frederick, PJ;Odunsi, K; | Efficacy and Safety of Pembrolizumab in Combination With Bevacizumab and Oral Metronomic Cyclophosphamide in the Treatment of Recurrent Ovarian Cancer: A Phase 2 Nonrandomized Clinical Trial | JAMA oncology | 33211063 | A modified proportion score threshold of at least 1% using the 22C3 antibody (QualTekMolecular Laboratories) was adopted similarly to previously described protocols.6 | Full Paper |
2020 | Jabbour, SK;Berman, AT;Decker, RH;Lin, Y;Feigenberg, SJ;Gettinger, SN;Aggarwal, C;Langer, CJ;Simone, CB;Bradley, JD;Aisner, J;Malhotra, J; | Phase 1 Trial of Pembrolizumab Administered Concurrently With Chemoradiotherapy for Locally Advanced Non-Small Cell Lung Cancer: A Nonrandomized Controlled Trial | JAMA Oncol | 32077891 | Exploratory analyses included PD-L1 status, by mean proportion score. Immunohistochemical staining for PD-L1 used a mouse monoclonal anti-PD-L1 antibody (clone 22C3).21 Furthermore, TILs were scored on a continuum from 0 to 3 (0 indicates absent TILs | Full Paper |
2020 | Kelly, CM;Antonescu, CR;Bowler, T;Munhoz, R;Chi, P;Dickson, MA;Gounder, MM;Keohan, ML;Movva, S;Dholakia, R;Ahmad, H;Biniakewitz, M;Condy, M;Phelan, H;Callahan, M;Wong, P;Singer, S;Ariyan, C;Bartlett, EK;Crago, A;Yoon, S;Hwang, S;Erinjeri, JP;Qin, LX;Tap, WD;D'Angelo, SP; | Objective Response Rate Among Patients With Locally Advanced or Metastatic Sarcoma Treated With Talimogene Laherparepvec in Combination With Pembrolizumab: A Phase 2 Clinical Trial | JAMA Oncol | 31971541 | Paraffin-embedded tumor material taken before and after treatment was examined for TIL immune biomarker expression, including PD-L1, PD-1, CD3, CD4, FOXP3, CD8, CD68, CD163, and MHC1 by Qualtek Laboratory. Programmed death-ligand 1 tumor membranous e | Full Paper |
2020 | Mancuso, F;Ogbah, Z;Fasani, R;Lo Giacco, D;Ramos, L;Oaknin, A;Alsina, M;Elez, E;Mercade, T;Saura, C;Capdevila, J;Vieito, M;Felip, E;Dienstmann, R;Gros, A;Seoane, J;Nuciforo, P;Tabernero, J;Garralda, E;Vivancos, A; | 581P VHIO immune gene expression profiling (VIGex) panel, a tool to explore tumour immune microenvironment | Annals of Oncology | self), Speaker Bureau/Expert testimony, Research grant/Funding (self), Travel/Accommodation/ Expenses: Novartis; Honoraria (self), Speaker Bureau/Expert testimony, Research grant/Funding (self), Travel/Accommodation/Expenses: Targos Molecular Patholo | Full Paper | |
2020 | Jotte, R;Cappuzzo, F;Vynnychenko, I;Stroyakovskiy, D;Rodríguez-Abreu, D;Hussein, M;Soo, R;Conter, HJ;Kozuki, T;Huang, KC;Graupner, V;Sun, SW;Hoang, T;Jessop, H;McCleland, M;Ballinger, M;Sandler, A;Socinski, MA; | Atezolizumab in Combination With Carboplatin and Nab-Paclitaxel in Advanced Squamous NSCLC (IMpower131): Results From a Randomized Phase III Trial | J Thorac Oncol | 32302702 | Skip to Main Content ;This work was supported by F. Hoffmann-La Roche Ltd/Genentech, Inc., a member of the Roche Group. The authors would like to acknowledge Targos Molecular Pathology GmbH (Kassel, Germany) for performing central PD-L1 and T-ef | Full Paper |
2020 | M.C.Liu, G.R.Oxnard, E.A.Klein, C.Swanton, M.V.Seiden | Sensitive and specific multi-cancer detection and localization using methylation signatures in cell-free DNA | Annals of Oncology | Background: Early cancer detection could identify tumors at a time when outcomes are superior and treatment is less morbid. This prospective case-control sub-study (from NCT02889978 and NCT03085888) assessed the performance of targeted methylation an | Full Paper | |
2020 | Taylor, WC; | Comment on "Sensitive and specific multi-cancer detection and localization using methylation signatures in cell-free DNA"" by M. C. Liu et al" | Ann. Oncol. | 32371122 | cfDNA [n = 2628 (1493 cancer; 1135 non-cancer)], formalin fixed paraffin embedded (FFPE) tumor biopsies (n = 242), and WBCs (n = 70) from the first CCGA sub-study; commercial tissue or cells [n = 227; Discovery Life Sciences (formerly Conversant Biol | Full Paper |
2020 | Draz, MS;Vasan, A;Muthupandian, A;Kanakasabapathy, MK;Thirumalaraju, P;Sreeram, A;Krishnakumar, S;Yogesh, V;Lin, W;Yu, XG;Chung, RT;Shafiee, H; | Virus detection using nanoparticles and deep neural network-enabled smartphone system | Science advances | 33328239 | Deidentified HCV-infected and HBV-infected patient plasma/ serum samples were obtained from Discovery Life Sciences Inc | Full Paper |
2020 | Chung, HC;Piha-Paul, SA;Lopez-Martin, J;Schellens, JHM;Kao, S;Miller, WH;Delord, JP;Gao, B;Planchard, D;Gottfried, M;Zer, A;Jalal, SI;Penel, N;Mehnert, JM;Matos, I;Bennouna, J;Kim, DW;Xu, L;Krishnan, S;Norwood, K;Ott, PA; | Pembrolizumab After Two or More Lines of Previous Therapy in Patients With Recurrent or Metastatic SCLC: Results From the KEYNOTE-028 and KEYNOTE-158 Studies | J Thorac Oncol | 31870883 | In KEYNOTE-028, tumor samples were assessed using a laboratory-developed prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA) with the 22C3 antibody clone (Merck Sharp & Dohme Corp., a subsidiary of Merck & Co., Inc., Ken | Full Paper |
2020 | Freeman, ZT;Nirschl, TR;Hovelson, DH;Johnston, RJ;Engelhardt, JJ;Selby, MJ;Kochel, CM;Lan, RY;Zhai, J;Ghasemzadeh, A;Gupta, A;Skaist, AM;Wheelan, SJ;Jiang, H;Pearson, AT;Snyder, LA;Korman, AJ;Tomlins, SA;Yegnasubramanian, S;Drake, CG; | A conserved intratumoral regulatory T cell signature identifies 4-1BB as a pan-cancer target | J. Clin. Invest. | 32015231 | Study approval. All human tumors were collected with approval from the Johns Hopkins University IRB with the following protocol IDs: renal clear cell carcinoma (IRB00033839), prostate cancer (NA_00082175), bladder cancer (NA_00026693), and glioblasto | Full Paper |
2020 | Weiss, J;Sheth, S;Deal, AM;Grilley Olson, JE;Patel, S;Hackman, TG;Blumberg, JM;Galloway, T;Patel, S;Zanation, AM;Shen, CJ;Hayes, DN;Hilliard, C;Mehra, R;McKinnon, KP;Wang, HH;Weissler, MC;Bauman, JR;Chera, BS;Vincent, BG; | Concurrent definitive immunoradiotherapy for patients with Stage III-IV Head and Neck Cancer and Cisplatin contraindication | Clin. Cancer Res. | 32371539 | withdrawal of permission. Correlative Analysis PDL1 staining was conducted by Qualtek Molecular Laboratories (Newtown, PA) as previously described 5 and required by Merck in supported investigator initiated trials. For both | Full Paper |
2020 | Graff, JN;Beer, TM;Alumkal, JJ;Slottke, RE;Redmond, WL;Thomas, GV;Thompson, RF;Wood, MA;Koguchi, Y;Chen, Y;Latour, E;Bergan, RC;Drake, CG;Moran, AE; | A phase II single-arm study of pembrolizumab with enzalutamide in men with metastatic castration-resistant prostate cancer progressing on enzalutamide alone | J Immunother Cancer | 32616555 | retained. Formalin-fixed, paraffin-embedded tumor samples were sent to a commercial vendor (Qualtek) for PD-L1 quantification by immunohistochemistry (clone 22C3). Additional methods details are described elsewhere.30 | Full Paper |
2020 | Majd, N;Waguespack, S;Janku, F;Fu, S;Penas-Prado, M;Xu, M;Alshawa, A;Kamiya-Matsuoka, C;Raza, S;McCutcheon, I;Naing, A; | Efficacy of pembrolizumab in patients with pituitary carcinoma: report of four cases from a phase II study | Journal for ImmunoTherapy of Cancer | PD-L1 staining was performed by Qualtek using Merck 22C3 antibody for PD-L1 | Full Paper | |
2020 | Lokeshwar, VB;Morera, DS;Hasanali, SL;Yates, TJ;Hupe, MC;Knapp, J;Lokeshwar, SD;Wang, J;Hennig, MJP;Baskar, R;Escudero, DO;Racine, RR;Dhir, N;Jordan, AR;Hoye, K;Azih, I;Manoharan, M;Klaassen, Z;Kavuri, S;Lopez, LE;Ghosh, S;Lokeshwar, BL; | A Novel Splice Variant of HYAL-4 Drives Malignant Transformation and Predicts Outcome in Patients with Bladder Cancer | Clin. Cancer Res. | 32094233 | Present address: Travis Yates: QualTek Molecular Laboratories, King of Prussia, PA; Diogo Escudero: George Washington University Kelly Hoye: University of North Carolina Lineberger Comprehensive Cancer Center; Ronny Racine: Astellas Institute for Reg | Full Paper |
2020 | Fong, LY;Taccioli, C;Palamarchuk, A;Tagliazucchi, GM;Jing, R;Smalley, KJ;Fan, S;Altemus, J;Fiehn, O;Huebner, K;Farber, JL;Croce, CM; | Abrogation of esophageal carcinoma development in miR-31 knockout rats | Proc. Natl. Acad. Sci. U.S.A. | 32123074 | lia isolated using the DNeasy Blood & Tissue Kit (Qiagen). WGS. DNA library preparation, library quality control, and nonhuman WGS to yield ∼30× coverage were performed on the Illumina HiSeq X at HudsonAlpha Genomic Services Laboratory. WGS Analysi | Full Paper |
2020 | Rodriguez, CP;Wu, QV;Voutsinas, J;Fromm, JR;Jiang, X;Pillarisetty, VG;Lee, SM;Santana-Davila, R;Goulart, B;Baik, CS;Chow, LQM;Eaton, K;Martins, R; | A Phase II Trial of Pembrolizumab and Vorinostat in Recurrent Metastatic Head and Neck Squamous Cell Carcinomas and Salivary Gland Cancer | Clin. Cancer Res. | 31796519 | Submission of archived tissue was required prior to study entry. In patient with no available archived tissue, fresh tissue biopsies were recommended. Biopsies were attempted after three cycles of therapy were completed, in patients who consented. PD | Full Paper |
2020 | Huetter, J;Gritzan, U;Gutcher, I;Doecke, WD;Luetke-Eversloh, MV;Golfier, S;Roider, HG;Frisk, AL;Hunter, J;Pow, A;Drake, A;Levine, Z;Levy, O;Azulay, M;Barbiro, I;Cojocaru, G;Vaknin, I;Kreft, B;Roese, L; | Characterization of BAY 1905254, an immune checkpoint inhibitor targeting the immunoglobulin-like domain containing receptor 2 (ILDR2) | Cancer Immunol Res | 32312711 | cancer patients, and commercially available cryopreserved human lymph node single- cell preparations from melanoma patients (Folio Conversant, USA) using the PrimeFlow™ RNA Assay technology (Invitrogen, CA, USA). Following enzymatic | Full Paper |
2020 | Ozkan-Dagliyan, I;Diehl, JN;George, SD;Schaefer, A;Papke, B;Klotz-Noack, K;Waters, AM;Goodwin, CM;Gautam, P;Pierobon, M;Peng, S;Gilbert, TSK;Lin, KH;Dagliyan, O;Wennerberg, K;Petricoin, EF;Tran, NL;Bhagwat, SV;Tiu, RV;Peng, SB;Herring, LE;Graves, LM;Sers, C;Wood, KC;Cox, AD;Der, CJ; | Low-Dose Vertical Inhibition of the RAF-MEK-ERK Cascade Causes Apoptotic Death of KRAS Mutant Cancers | Cell Rep | 32553168 | CRISPR sequencing was performed at UNC (High-Throughput Sequencing Facility, Chapel Hill, NC) and Hudson Alpha (HudsonAlpha Genomic Services Laboratory, Huntsville, AL) | Full Paper |
2020 | Wang, X;Kelkar, YD;Xiong, X;Martinson, EO;Lynch, J;Zhang, C;Werren, JH;Wang, X; | Genome Report: Whole Genome Sequence and Annotation of the Parasitoid Jewel Wasp Nasonia giraulti Laboratory Strain RV2X[u] | G3 (Bethesda) | 32571804 | and Helen Wisch Professorship. X.X. is supported by the Auburn University Presidential Graduate Research Fellowship and Auburn University College of Veterinary Medicine Dean’s Fellowship. We thank HudsonAlpha Discovery for assistance with Illumina se | Full Paper |
2020 | Ulasov, I;Borovjagin, A;Fares, J;Yakushov, S;Malin, D;Timashev, P;Lesniak, MS; | MicroRNA 345 (miR345) regulates KISS1-E-cadherin functional interaction in breast cancer brain metastases | Cancer Lett. | 32246957 | One hundred and eleven tumor samples, representing invasive ductal carcinoma (IDC) were subjected for evaluation as a part of brain tissue microarrays presented by: US Biomax (BR812), Folio Biosciences (AY-HH0137) and biobank at the University of Chi | Full Paper |
2020 | Chiang, J;Ahmed, N;Foote, J;Prasad, N;Karri, S;Pagidas, K;Wiltshire, A;Gunn, D;Singh, K; | OVARIAN AGING AND REPRODUCTIVE SENESCENCE IN MOUSE MODEL OF MITOCHONDRIAL DYSFUNCTION | Fertility and Sterility | 2 HudsonAlpha Discovery, Huntsville, AL; 3 TCM University, Lincoln, RI | Full Paper | |
2020 | Ho, AY;Barker, CA;Arnold, BB;Powell, SN;Hu, ZI;Gucalp, A;Lebron-Zapata, L;Wen, HY;Kallman, C;D'Agnolo, A;Zhang, Z;Flynn, J;Dunn, SA;McArthur, HL; | A phase 2 clinical trial assessing the efficacy and safety of pembrolizumab and radiotherapy in patients with metastatic triple-negative breast cancer | Cancer | 31747077 | PD‐L1 tumor expression was evaluated in prospectively collected samples of metastatic biopsies by a central laboratory (QualTek Molecular Laboratories, Goleta, California) using a previously described prototype assay using the 22C3 antibody (Merck an | Full Paper |
2020 | Kasimir-Bauer, S;Roder, J;Obermayr, E;Mahner, S;Vergote, I;Loverix, L;Braicu, E;Sehouli, J;Concin, N;Kimmig, R;Net, L;Roder, H;Zeillinger, R;Aust, S;On Behalf Of The Ovcad Ovarian Cancer Diagnosis Initiative, ; | Definition and Independent Validation of a Proteomic-Classifier in Ovarian Cancer | Cancers | 32899818 | These samples were obtained from commercial biobanks (Conversant Bio (Huntsville, AL, USA) and Oncology Metrics (Forth Worth, TX, USA)), under their ethics-approved protocols | Full Paper |
2020 | Jimenez, C;Subbiah, V;Stephen, B;Ma, J;Milton, D;Xu, M;Zarifa, A;Akhmedzhanov, FO;Tsimberidou, A;Habra, MA;Rodon Anhert, J;Fu, S;Naing, A; | Phase II Clinical Trial of Pembrolizumab in Patients with Progressive Metastatic Pheochromocytomas and Paragangliomas | Cancers | 32824391 | tissue samples or newly obtained biopsy specimens (if archival tissue was not available) were evaluated by immunohistochemical analysis to determine the expression of PDL-1 in tumor cells and tumor-infiltrating mononuclear inflammatory cells by Qualt | Full Paper |
2020 | Jordan, AR;Wang, J;Yates, TJ;Hasanali, SL;Lokeshwar, SD;Morera, DS;Shamaladevi, N;Li, CS;Klaassen, Z;Terris, MK;Thangaraju, M;Singh, AB;Soloway, MS;Lokeshwar, VB; | Molecular targeting of renal cell carcinoma by an oral combination | Oncogenesis | 32427869 | Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, 1410 Laney Walker Blvd., Augusta, GA, 30912, USA.||Sheila and David Fuente Graduate Program in Cancer Biology, University of Miami-Miller School of Medi | Full Paper |
2020 | Mehnert, JM;Bergsland, E;O'Neil, BH;Santoro, A;Schellens, JHM;Cohen, RB;Doi, T;Ott, PA;Pishvaian, MJ;Puzanov, I;Aung, KL;Hsu, C;Le Tourneau, C;Hollebecque, A;Élez, E;Tamura, K;Gould, M;Yang, P;Stein, K;Piha-Paul, SA; | Pembrolizumab for the treatment of programmed death-ligand 1-positive advanced carcinoid or pancreatic neuroendocrine tumors: Results from the KEYNOTE-028 study | Cancer | 32320048 | PD was confirmed by repeat radiographic imaging ≥4 weeks after the initial scan. Investigators could opt to keep clinically stable patients on treatment until confirmed PD. Safety was monitored through 30 days after the last treatment dose (90 days f | Full Paper |
2020 | Wen, X;Ou, Y;Zarick, H;Zhang, X;Hmelo, A;Victor, Q;Paul, E;Slocik, J;Naik, R;Bellan, L;Lin, E;Bardhan, R; | PRADA: Portable Reusable Accurate Diagnostics with nanostar Antennas for multiplexed biomarker screening | Bioeng Transl Med | labor intensive, and cost prohibitive.39,40 We chose to measure the accuracy of PRADA in commercially available de-identified human patient serum (Discovery Life Sciences Inc.) to recapitulate clinical diagnostics where biomarkers of interest compete | Full Paper | |
2020 | Gonzalez-Ericsson, PI;Stovgaard, ES;Sua, LF;Reisenbichler, E;Kos, Z;Carter, JM;Michiels, S;Le Quesne, J;Nielsen, TO;Laenkholm, AV;Fox, SB;Adam, J;Bartlett, JM;Rimm, DL;Quinn, C;Peeters, D;Dieci, MV;Vincent-Salomon, A;Cree, I;Hida, AI;Balko, JM;Haynes, HR;Frahm, I;Acosta-Haab, G;Balancin, M;Bellolio, E;Yang, W;Kirtani, P;Sugie, T;Ehinger, A;Castaneda, CA;Kok, M;McArthur, H;Siziopikou, K;Badve, S;Fineberg, S;Gown, A;Viale, G;Schnitt, SJ;Pruneri, G;Penault-Llorca, F;Hewitt, S;Thompson, EA;Allison, KH;Symmans, WF;Bellizzi, AM;Brogi, E;Moore, DA;Larsimont, D;Dillon, DA;Lazar, A;Lien, H;Goetz, MP;Broeckx, G;El Bairi, K;Harbeck, N;Cimino-Mathews, A;Sotiriou, C;Adams, S;Liu, SW;Loibl, S;Chen, IC;Lakhani, SR;Juco, JW;Denkert, C;Blackley, EF;Demaria, S;Leon-Ferre, R;Gluz, O;Zardavas, D;Emancipator, K;Ely, S;Loi, S;Salgado, R;Sanders, M;, ; | The path to a better biomarker: application of a risk management framework for the implementation of PD-L1 and TILs as immuno-oncology biomarkers in breast cancer clinical trials and daily practice | J. Pathol. | 32129476 | PANACEA NCT02129556 58 Pembrolizumab + trastuzumab single arm phase Ib/II PreTx LAdv or mHER2+ BC Ib: PD‐L1+ (6) II: PD‐L1+ & PD‐L1‐ (52) PD‐L1 (QualTek/ 22C3) tested prospectively at BTx (58). TILs were evaluated retrospectively (48). II: H | Full Paper |
2020 | Lu, H;Molony, RD;Chen, D;Jang, S;Wolf, B;Ewert, S;Flaherty, M;Xu, F;Isim, S;Shim, Y;Dornelas, C;Balke, N;Leber, XC;Scharenberg, M;Koelln, J;Choi, E;Ward, R;Johnson, J;Calzascia, T;Isnardi, I;Williams, JA;Lindenbergh, PL;van de Donk, NWCJ;Mutis, T;Huet, H;Lees, E;Meyer, MJ; | Development of anti-CD32b antibodies with enhanced Fc function for the treatment of B and plasma cell malignancies | Mol. Cancer Ther. | 32847974 | detect CD32b expression on primary cells and cell lines. Patient samples for CD32b expression analysis were purchased from Conversant Bio, now part of Discovery Life Science. 2B6 binding to normal human plasma cells from | Full Paper |
2020 | Hutchins, B;Starling, GC;McCoy, MA;Herzyk, D;Poulet, FM;Dulos, J;Liu, L;Kang, SP;Fayadat-Dilman, L;Hsieh, M;Andrews, CL;Ayanoglu, G;Cullen, C;Malefyt, RW;Kastelein, RA;Saux, SL;Lee, J;Li, S;Malashock, D;Sadekova, S;Soder, G;van Eenennaam, H;Willingham, A;Yu, Y;Streuli, M;Carven, GJ;van Elsas, A; | Biophysical and Immunological Characterization and In Vivo Pharmacokinetics and Toxicology in Nonhuman Primates of the Anti-PD-1 Antibody Pembrolizumab | Molecular cancer therapeutics | 32229606 | See Supplementary Data for additional details. Primary tumor cell culture assay Fresh non-small cell lung cancer (NSCLC) tumor tissues were collected and shipped overnight in AQIX media (AQIX) by collection providers (Folio Biosciences, Bio-Options, | Full Paper |
2020 | Smith, SD;Till, BG;Shadman, MS;Lynch, RC;Cowan, AJ;Wu, QV;Voutsinas, J;Rasmussen, HA;Blue, K;Ujjani, CS;Shustov, A;Cassaday, RD;Fromm, JR;Gopal, AK; | Pembrolizumab with R-CHOP in previously untreated diffuse large B-cell lymphoma: potential for biomarker driven therapy | Br. J. Haematol. | 32030732 | PD‐L1 immunohistochemistry was performed centrally by QualTek Molecular Laboratories using the mouse monoclonal antibody clone 22c3 by previously described methods (Supplementary Appendix, Section B) (Dolled‐Filhart et al. , 2016). Statistical consid | Full Paper |
2020 | Vijayvergia, N;Dasari, A;Deng, M;Litwin, S;Al-Toubah, T;Alpaugh, RK;Dotan, E;Hall, MJ;Ross, NM;Runyen, MM;Denlinger, CS;Halperin, DM;Cohen, SJ;Engstrom, PF;Strosberg, JR; | Pembrolizumab monotherapy in patients with previously treated metastatic high-grade neuroendocrine neoplasms: joint analysis of two prospective, non-randomised trials | Br. J. Cancer | 32152503 | For patients enrolled into FC, PD-L1 immunohistochemistry (IHC) assay was performed at Qualtek Research Laboratories on formalin-fixed, paraffin-embedded tissue sections using anti-PD-L1 monoclonal antibody clone 22C3 [Merck Research Laboratories] | Full Paper |
2020 | Schruf, E;Schroeder, V;Le, HQ;Schönberger, T;Raedel, D;Stewart, EL;Fundel-Clemens, K;Bluhmki, T;Weigle, S;Schuler, M;Thomas, MJ;Heilker, R;Webster, MJ;Dass, M;Frick, M;Stierstorfer, B;Quast, K;Garnett, JP; | Recapitulating idiopathic pulmonary fibrosis related alveolar epithelial dysfunction in a human iPSC-derived air-liquid interface model | FASEB J. | 32297676 | Formalin-fixed and paraffin embedded lung samples from human IPF patients were purchased from Folio Biosciences (Powell, OH, US) under the regulatory conditions of the Boehringer Ingelheim corporate policy regarding the acquisition and use of human b | Full Paper |
2020 | Kang, J;Yeom, G;Jang, H;Park, C;Kim, M; | Highly sensitive and universal detection strategy based on a colorimetric assay using target-specific heterogeneous sandwich DNA aptamer | Analytica Chimica Acta | All reagent concentrations were the same as those analyzed in the developed method. 2.8. Evaluation of clinical samples. We obtained patient samples containing NPs of influenza A and B viruses from Discovery Life Sciences, Inc (Huntsville, AL, USA) | Full Paper | |
2020 | Seyger, M;Abramovits, W;Liljedahl, M;Hoejen, MN;Teng, J; | Safety and efficacy of fixed-dose combination calcipotriol (50 μg/g) and betamethasone dipropionate (0.5 mg/g) cutaneous foam in adolescent patients (aged 12 to <17 years) with plaque psoriasis: results of a phase II, open-label trial | J Eur Acad Dermatol Venereol | 32074665 | W. Abramovits has received honoraria or fees for serving on advisory boards as a speaker and as a consultant, and has received grants as an investigator from AbbVie, Akros, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant Bio | Full Paper |
2020 | Piha-Paul, SA;Oh, DY;Ueno, M;Malka, D;Chung, HC;Nagrial, A;Kelley, RK;Ros, W;Italiano, A;Nakagawa, K;Rugo, HS;de Braud, F;Varga, AI;Hansen, A;Wang, H;Krishnan, S;Norwood, KG;Doi, T; | Efficacy and safety of pembrolizumab for the treatment of advanced biliary cancer: Results from the KEYNOTE-158 and KEYNOTE-028 studies | Int. J. Cancer | 32359091 | In KEYNOTE‐028, a prototype immunohistochemistry assay (QualTek, Goleta, California) was used to prospectively detect PD‐L1 expression, with positivity defined as membranous PD‐L1 expression in ≥1% of tumor and associated inflammatory cells or positi | Full Paper |
2020 | Agnello, G;Alters, SE;Rowlinson, SW; | Preclinical safety and antitumor activity of the arginine-degrading therapeutic enzyme pegzilarginase, a PEGylated, cobalt-substituted recombinant human arginase 1 | Transl Res | 31954097 | CD8 IHC analysis of FFPE sections was performed by QualTek Molecular Laboratories (Goleta, Calif) and LC3B IHC analysis of FFPE sections was performed by GoPath Laboratories (Buffalo Grove, Ill). Statistical analyses | Full Paper |
2020 | Ross, AE;Hurley, PJ;Tran, PT;Rowe, SP;Benzon, B;Neal, TO;Chapman, C;Harb, R;Milman, Y;Trock, BJ;Drake, CG;Antonarakis, ES; | A pilot trial of pembrolizumab plus prostatic cryotherapy for men with newly diagnosed oligometastatic hormone-sensitive prostate cancer | Prostate Cancer Prostatic Dis. | 31611635 | pathologist. Post-treatment biopsies were evaluated for PD-L1, CD4, CD8, and FoxP3 proteins by single-stain semiquantitative immunohistochemistry performed by QualTek Molecular and Clinical Laboratories (Santa Barbara, CA) ;re assessed by a geni | Full Paper |
2020 | Scheffler, M;Holzem, A;Kron, A;Nogova, L;Ihle, MA;von Levetzow, C;Fassunke, J;Wömpner, C;Bitter, E;Koleczko, S;Abdulla, DSY;Michels, S;Fischer, R;Riedel, R;Weber, JP;Westphal, T;Gerigk, U;Kern, J;Kaminsky, B;Randerath, W;Kambartel, KO;Merkelbach-Bruse, S;Büttner, R;Wolf, J; | Co-occurrence of targetable mutations in Non-small cell lung cancer (NSCLC) patients harboring MAP2K1 mutations | Lung Cancer | 32361034 | Reinhard Büttner: RB has been a consultant for Pfizer and Novartis regarding MET inhibitors and is a co-founder and CSO of Targos Molecular Pathology, Kassel Germany | Full Paper |
2020 | Lee, EK;Xiong, N;Cheng, SC;Barry, WT;Penson, RT;Konstantinopoulos, PA;Hoffman, MA;Horowitz, N;Dizon, DS;Stover, EH;Wright, AA;Campos, SM;Krasner, C;Morrissey, S;Whalen, C;Quinn, R;Matulonis, UA;Liu, JF; | Combined pembrolizumab and pegylated liposomal doxorubicin in platinum resistant ovarian cancer: A phase 2 clinical trial | Gynecol. Oncol. | 32771276 | Tumor infiltrating lymphocytes (TILs) were estimated as an overall average of the sample and reported on a scale of 0 (absent TILs) to 3 (profuse infiltration). PD-L1 and TIL analysis was performed at QualTek Molecular Laboraties, PA | Full Paper |
2020 | Frumovitz, M;Westin, SN;Salvo, G;Zarifa, A;Xu, M;Yap, TA;Rodon, AJ;Karp, DD;Abonofal, A;Jazaeri, AA;Naing, A; | Phase II study of pembrolizumab efficacy and safety in women with recurrent small cell neuroendocrine carcinoma of the lower genital tract | Gynecol. Oncol. | 32534809 | Adverse events were assessed using the National Cancer Institute Common Terminology Criteria for Adverse Events version 4.03. When fresh or archived tissue was available, PD-L1 biomarker analysis was performed by Qualtek assay using Merck 22C3 antibo | Full Paper |
2020 | Gaillard, S;Broadwater, G;Andrews, W;Starr, M;Previs, R;Havrilesky, L;Davidson, B;Berchuck, A;Yi, J;Nixon, A;Secord, A; | Pembrolizumab window study: Illuminating the immunologic landscape in gynecologic cancers | Gynecologic Oncology | changes in PD-L1 expression (by immunohistochemistry with monoclonal antibody clone 22C3 on FFPE specimens;l Qualtek, PA) scored using a quantitative modified proportion score (MPS) | Full Paper | |
2020 | Pillarisetti, K;Powers, G;Luistro, L;Babich, A;Baldwin, E;Li, Y;Zhang, X;Mendonça, M;Majewski, N;Nanjunda, R;Chin, D;Packman, K;Elsayed, Y;Attar, R;Gaudet, F; | Teclistamab is an active T cell-redirecting bispecific antibody against B-cell maturation antigen for multiple myeloma | Blood Adv | 32956453 | Plasma samples were from normal subjects (n = 39) and patients with MM (n = 40); donors were acquired from Conversant Biologics and subjected to proteolysis and subsequent mass spectrometry using a reference multiple reaction monitoring sequence (YCN | Full Paper |
2020 | Svrzikapa, N;Longo, K;Prasad, N;Boyanapalli, R;Brown, J;Dorset, D;Yourstone, S;Powers, J;Levy, S;Morris, A;Vargeese, C;Goyal, J; | Investigational assay for haplotype phasing of the huntingtin gene | Molecular Therapy - Methods & Clinical Development | 4 1 Wave Life Sciences Ltd., Cambridge, MA, 02138 USA; 2 Department of Paediatrics, Medical 5 Sciences Division, University of Oxford, Oxford, OX3 9DU UK; 3 HudsonAlpha Discovery, 6 Discovery Life Sciences, Huntsville, AL, 35806 USA; 4 Genomic Servic | Full Paper | |
2020 | Sebest, P;Fojt, L;Ostatna, V;Fojta, M;Danhel, A; | Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein | Bioelectrochemistry | 31855832 | electrode setup composed of: i) a bPGE or edge-plane PGE (ePGE, both Momentive) working electrode, together with ii) a refillable miniature Ag/AgCl/3M KCl reference electrode (eDAQ) placed into the salt bridge composed of a heat shrink Teflon tube (Q | Full Paper |
2020 | Xicoy, H;Brouwers, JF;Wieringa, B;Martens, GJM; | Explorative Combined Lipid and Transcriptomic Profiling of Substantia Nigra and Putamen in Parkinson's Disease | Cells | 32858884 | Total RNA samples were spectrophotometrically analyzed and their 260/280 ratio was typically above 1.9. RNA-seq directionality library preparation and sequencing with Poly (A) selection was performed by HudsonAlpha Genomic Services Laboratory (Huntsv | Full Paper |
2020 | Elgharably, H;Okamoto, T;Ayyat, KS;Niikawa, H;Meade, S;Farver, C;Chan, ER;Aldred, MA;McCurry, KR; | Human Lungs Airway Epithelium Upregulate MicroRNA-17 and MicroRNA-548b in Response to Cold Ischemia and Ex Vivo Reperfusion | Transplantation | 32590607 | We thank Tracey Bonfield, PhD from CWRU for performing the cytokines analysis. We thank Gerard J. Nuovo, MD, and Adel Mikhail, PhD from Phylogeny Inc. for performing the in situ hybridization assays of the microRNA | Full Paper |
2020 | Grigorieva, J;Asmellash, S;Net, L;Tsypin, M;Roder, H;Roder, J; | Mass Spectrometry-Based Multivariate Proteomic Tests for Prediction of Outcomes on Immune Checkpoint Blockade Therapy: The Modern Analytical Approach | Int J Mol Sci | 32012941 | 50] PSEA reference set NSCLC patients; samples obtained from commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrix (Fort Worth, TX) 100 Grigorieva et al., 2019 [51] 3. BDX008 Test and ICB Test BDX008 | Full Paper |
2020 | Awty-Carroll, D;Ravella, S;Clifton-Brown, J;Robson, P; | Using a Taguchi DOE to investigate factors and interactions affecting germination in Miscanthus sinensis | Sci Rep | 32005862 | All dishes were prepared and tested at the same time to limit extraneous sources of variation. The Taguchi design of experiment (DOE) was produced and primarily analysed using Qualtek-4 (Nutek inc., Michigan, USA) with secondary analysis in R34 ;Ful | Full Paper |
2020 | Noto, PB;Sikorski, TW;Zappacosta, F;Wagner, CD;Montes de Oca, R;Szapacs, ME;Annan, RS;Liu, Y;McHugh, CF;Mohammad, HP;Piccoli, SP;Creasy, CL; | Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissues | Scientific reports | 33335114 | nd Treatment of Laboratory Animals and were reviewed by the Institutional Animal Care and Use Committee at GSK. Immunohistochemistry Both total and ADM-R225-hnRNP-A1 IHC assays were developed and at QualTek Molecular Laboratories using anti-hnRNP-A1 | Full Paper |
2020 | Rüschoff, J;Lebeau, A;Sinn, P;Schildhaus, HU;Decker, T;Ammann, J;Künzel, C;Koch, W;Untch, M; | Statistical modelling of HER2-positivity in breast cancer: Final analyses from two large, multicentre, non-interventional studies in Germany | Breast | 31918324 | Institut für Pathologie Nordhessen and Targos Molecular Pathology GmbH, Germaniastr. 7, D-34119, Kassel, Germany. Electronic address: rueschoff@patho-nordhessen.de.++Institut für Pathologie, Universitätsklinikum Hamburg-Eppendorf, Martinistr. 52, 202 | Full Paper |
2020 | Emancipator, K; | Keytruda and PD-L1: a Real-World Example of Co-development of a Drug with a Predictive Biomarker | The AAPS journal | 33222057 | two clinical trial assays were used (Table I). The first, described in the literature as the "prototype assay" (4), was developed in-house at Merck & Co., Inc., Kenilworth, NJ, USA, and eventually placed and refined in an accredited clinical laborato | Full Paper |
2020 | Gebauer, F;Krämer, M;Bruns, C;Schlößer, HA;Thelen, M;Lohneis, P;Schröder, W;Zander, T;Alakus, H;Buettner, R;Loeser, H;Quaas, A; | Lymphocyte activation gene-3 (LAG3) mRNA and protein expression on tumour infiltrating lymphocytes (TILs) in oesophageal adenocarcinoma | J. Cancer Res. Clin. Oncol. | 32592066 | Reinhard Büttner: Co-Founder and Co-Owner of Targos Molecular Pathology Inc. Kassel/Germany. Hans Schlösser: Funding for Research from Astra Zeneca for research outside this study. Thomas Zander: BMS: advisory board, clinical trial; Novartis: advisor | Full Paper |
2020 | Wang, X;Jaimes, M;Gu, H;Shults, K;Putta, S;Sharma, V;Chow, W;Gogoi, P;Handique, K;Patterson, BK; | Cell by cell immuno- and cancer marker profiling of non-small cell lung cancer tissue: Checkpoint marker expression on CD103+, CD4+ T-cells predicts circulating tumor cells | Translational oncology | 33217647 | of a total of 72 biomarkers that result from using combinations of cellular markers in our algorithm to predict CTCs. Material and methods Samples Fresh tissues were obtained from 10 NSCLC cases by Folio Biosciences [Powell, OH] with paired peripher | Full Paper |
2020 | MacCarty, N;Bentson, S;Cushman, K;Au, J;Li, C;Murugan, G;Still, D; | Stratification of particulate matter in a kitchen: A comparison of empirical to predicted concentrations and implications for cookstove emissions targets | Energy for Sustainable Development | the top perimeter (Fig. 3). Small computer fans (Qualtek FAD1-06020CSHW11 rated at 1.44 W @12 V and 16 CFM) covered the holes at the top perimeter to force air out of the test kitchen at a constant rate. In Phase 1 of the | Full Paper | |
2020 | Jia, Y;Liu, L;Shan, B; | Future of immune checkpoint inhibitors: focus on tumor immune microenvironment | Annals of translational medicine | 33145314 | The TNBC cohort in KEYNOTE-012 phase Ib trial enrolled 32 cases of heavily pre-treated patients with PD-L1 positivity in the stroma or PD-L1 ≥1% tumor cells using their Qualtek assay | Full Paper |
2020 | Sebest, P;Ostatna, V;Fojta, M;Danhel, A; | Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles | Journal of Electroanalytical Chemistry | in three-electrode setup composed of: i) base plane PGE (bPGE,od 3.0 mm, Momentive) as working electrode, together with ii) refillable miniature Ag/AgCl/3 M KCl reference electrode (eDAQ) placed into the salt bridge composed of heat shrink Teflon tub | Full Paper | |
2020 | Rehkaemper, J;Korenkov, M;Quaas, A;Rueschoff, J;Pamuk, A;Zander, T;Hillmer, AM;Buettner, R;Hoelscher, AH;Bruns, CJ;Loeser, H;Alakus, H;Schoemig-Markiefka, B; | Amplification of KRAS and its heterogeneity in non-Asian gastric adenocarcinomas | BMC Cancer | 32571252 | Institute of Pathology, University Hospital Cologne, Cologne, Germany. jan.rehkaemper@uk-koeln.de.++Department of General, Visceral and Cancer Surgery, University Hospital Cologne, Cologne, Germany.++Institute of Pathology, University Hospital Cologn | Full Paper |
2020 | Montalban-Bravo, G;Class, CA;Ganan-Gomez, I;Kanagal-Shamanna, R;Sasaki, K;Richard-Carpentier, G;Naqvi, K;Wei, Y;Yang, H;Soltysiak, KA;Chien, K;Bueso-Ramos, C;Do, KA;Kantarjian, H;Garcia-Manero, G; | Transcriptomic analysis implicates necroptosis in disease progression and prognosis in myelodysplastic syndromes | Leukemia | 31719677 | BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). Patient characteristics are detailed in Supplementary Table S1. Informed consent was obtained Save | Full Paper |
2020 | Arumugam, A;Faron, ML;Yu, P;Markham, C;Wu, M;Wong, S; | A Rapid SARS-CoV-2 RT-PCR Assay for Low Resource Settings | Diagnostics (Basel, Switzerland) | 32987722 | No. 10006606) were purchased from Integrated DNA Technologies (Coralville, IA, USA). The clinical specimens of Influenza A (DLS16-85584), Influenza B (DLS15-33890), and RSV (KH19-00715) were obtained from Discovery Life Sciences Inc Cached | Full Paper |
2020 | Bio, S;Nunes, B; | Acute effects of diclofenac on zebrafish: Indications of oxidative effects and damages at environmentally realistic levels of exposure | Environ. Toxicol. Pharmacol. | 32320907 | With the increasing awareness about the contamination of the aquatic environment by pharmaceuticals, there is a growing need to study their adverse effects on aquatic organisms. Diclofenac is a non-steroidal anti-inflammatory drug (NSAID), whose wide | Full Paper |
2020 | Piedade, F;Bio, S;Nunes, B; | Effects of common pharmaceutical drugs (paracetamol and acetylsalicylic acid) short term exposure on biomarkers of the mussel Mytilus spp | Environ. Toxicol. Pharmacol. | 31704586 | Pharmaceutical drugs in the wild may pose significant risks to non-target exposed organisms. This situation is even more troublesome for coastal marine or estuarine environments, located in the vicinity of large human conglomerates, for which the put | Full Paper |
2020 | Lin, J;Song, AJ;Hoffman-Censits, J;Leiby, BE;Tuluc, M;Shaw, C;Harshyne, L;Kean, R;Bar-Ad, V;Den, RB;Hurwitz, MD;Louie, J;Philipose, S;Deshmukh, SP;Johnson, JM;Dicker, AP;Hooper, DC;Kelly, WK;Lu, B; | A Pilot Study of Radiation Therapy in Combination With Pembrolizumab in Patients With Metastatic Renal Cell Cancer | Am. J. Clin. Oncol. | 31693508 | When possible preradiation and post- radiation biopsy material was compared. Biopsy samples were analyzed by a QualTek pathologist (King of Prussia, PA) and Merck 22C3 antibody was used to score PD-L1 membrane expression | Full Paper |
2020 | Johnston, G;Ramsey, HE;Liu, Q;Wang, J;Stengel, KR;Sampathi, S;Acharya, P;Arrate, M;Stubbs, MC;Burn, T;Savona, MR;Hiebert, SW; | Nascent transcript and single-cell RNA-seq analysis defines the mechanism of action of the LSD1 inhibitor INCB059872 in myeloid leukemia | Gene | 32422235 | Samples were submitted to HudsonAlpha Genomic Services Laboratory for library preparation with Nugen Ovation RNA-Seq System V2 kit (cat# 7102-08) and sequencing on Illumina NovaSeq6000. Data analysis was performed as described above. 2.13 Data availa | Full Paper |
2020 | Lin, H;Damen, JE;Walasek, MA;Szilvassy, SJ;Turhan, AG;Louis, SA;Eaves, AC;Wognum, AW; | Feeder-free and serum-free in vitro assay for measuring the effect of drugs on acute and chronic myeloid leukemia stem/progenitor cells | Exp. Hematol. | 32798646 | CML. 1, Folio Conversant, 3.64 × 10 6, 4.9%, 81.2%, 15 × 10 3, 84, Male, Pre treatment, 112.2; 1764.4. 2, Folio Conversant, 3.17 × 10 6, 6.8%, 79.3%, 18.5 × 10 3, 70, Male, Pre treatment, 44.3; 2380.1. 3, Folio Conversant, 1.38 × 10 6, 18.8%, 78.7%, | Full Paper |
2020 | Barroso-Sousa, R;Krop, IE;Trippa, L;Tan-Wasielewski, Z;Li, T;Osmani, W;Andrews, C;Dillon, D;Richardson, ET;Pastorello, RG;Winer, EP;Mittendorf, EA;Bellon, JR;Schoenfeld, JD;Tolaney, SM; | A Phase II Study of Pembrolizumab in Combination With Palliative Radiotherapy for Hormone Receptor-positive Metastatic Breast Cancer | Clin. Breast Cancer | 32113750 | Clinical benefit is defined as complete response, partial response, or stable disease ≥ 24 weeks according to RECIST 1.1. Exploratory Biomarkers. Five patients had PD-L1 testing assessed centrally by QualTek using the 22C3 antibody | Full Paper |
2020 | Patel, JJ;Levy, DA;Nguyen, SA;Knochelmann, HM;Day, TA; | Impact of PD-L1 expression and human papillomavirus status in anti-PD1/PDL1 immunotherapy for head and neck squamous cell carcinoma-Systematic review and meta-analysis | Head Neck | 31762164 | Oro Valley, Arizona). One study28 did not specify the assay utilized for PD‐L1 expression, but rather reported the laboratory where analysis occurred (QualTek Molecular Laboratories, Goleta, California). This lab confirmed the | Full Paper |
2020 | Adekanmbi, EO;Giduthuri, AT;Srivastava, SK; | Dielectric Characterization and Separation Optimization of Infiltrating Ductal Adenocarcinoma via Insulator-Dielectrophoresis | Micromachines (Basel) | 32218322 | The DEP suspending medium (dextrose solution) was prepared and characterized as described in our previous article [38]. The prepared 100 mL suspending medium was divided into five separate beakers. Into each beaker, except the first, calculated volum | Full Paper |
2020 | Jasani, B;Bänfer, G;Fish, R;Waelput, W;Sucaet, Y;Barker, C;Whiteley, JL;Walker, J;Hovelinck, R;Diezko, R; | Evaluation of an online training tool for scoring programmed cell death ligand-1 (PD-L1) diagnostic tests for lung cancer | Diagn Pathol | 32303234 | Correspondence: rolf.diezko@targos-gmbh.de 2Advance - Training and Consulting Unit, Targos Molecular Pathology GmbH, Kassel, Germany Full list of author information is available at the end of the article Jasani et al ;Correspondence: rolf.diezko | Full Paper |
2020 | Schöniger, S;Degner, S;Zhang, Q;Schandelmaier, C;Aupperle-Lellbach, H;Jasani, B;Schoon, HA; | Tumor Infiltrating Lymphocytes in Pet Rabbit Mammary Carcinomas: A Study with Relevance to Comparative Pathology | Animals : an open access journal from MDPI | 32824521 | 1. Targos Molecular Pathology GmbH, Germaniastrasse 7, 34119 Kassel, Germany. 2. Institute of Veterinary Pathology, University of Leipzig, An den Tierkliniken, 04109 Leipzig, Germany. 3. Institute of Anatomy, Experimental Neurobiology Cached | Full Paper |
2020 | Schöniger, S;Schoon, HA; | The Healthy and Diseased Equine Endometrium: A Review of Morphological Features and Molecular Analyses | Animals : an open access journal from MDPI | 32260515 | Targos Molecular Pathology GmbH, Germaniastrasse 7, 34119 Kassel, Germany.++Institute of Veterinary Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig, Germany | Full Paper |
2020 | Fish, EJ;Martinez-Romero, EG;DeInnocentes, P;Koehler, JW;Prasad, N;Smith, AN;Bird, RC; | Circulating microRNA as biomarkers of canine mammary carcinoma in dogs | J. Vet. Intern. Med. | 32342546 | Extracted RNA was stored at −80°C until being shipped on dry ice to the Genomic Services Laboratory at HudsonAlpha Discovery for deep‐sequencing as previously described.14 Briefly, RNA libraries were processed extracted RNA using a NEBNext Small RNA | Full Paper |
2020 | Nuovo, G; | A broad-based approach to differentiate CIN from its mimics: The utility of in situ hybridization and immunohistochemistry | Ann Diagn Pathol | 32330660 | study was the review of 100 consecutive cases of formalin fixed, paraffin embedded tissues cervical biopsies done for either an abnormal Pap smear and/or a high risk HPV DNA positive result where the diagnosis was listed as CIN 1 or CIN 2. Folio Bios | Full Paper |
2020 | Inceer, H;Ozcan, M; | Taxonomic evaluations on the anatomical characters of leaf and achene in Turkish Tripleurospermum with its relative Matricaria (Asteraceae) | Flora | Besides, both genera have appeared well separated in different and supported clades based on molecular phylogeny (Inceer et al., 2018) | Full Paper | |
2020 | Roder, J;Net, L;Oliveira, C;Meyer, K;Asmellash, S;Kasimir-Bauer, S;Pass, H;Weber, J;Roder, H;Grigorieva, J; | A proposal for score assignment to characterize biological processes from mass spectral analysis of serum | Clinical Mass Spectrometry | Samples were obtained from the commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrics (Fort Worth, TX). b They were purchased from commercial biobanks, including Conversant Bio (Huntsville, AL), AdeptBio (Memphis, TN), and ProMedD | Full Paper | |
2020 | Köhne, M;Mönnig, F;Papin, J;Schöniger, S;Tönissen, A;Rohn, K;Martinsson, G;Schoon, HA;Sieme, H; | Effects of Hysteroscopic and Uterine Body Insemination on the Presence of Selected Immune Cells in the Equine Endometrium | J. Equine Vet. Sci. | 32534786 | Current affiliation: Targos Molecular Pathology GmbH, 34119 Kassel, Germany | Full Paper |
2020 | Schildhaus, HU; | [Immunohistochemistry-based predictive biomarkers for lung cancer] | Pathologe | 31989233 | Interessenkonflikt. H.-U. Schildhaus erhielt Honorare von MSD, BMS, Abbvie, ZytoVision, Zytomed, Pfizer, Abbott Molecular, Novartis Oncology und Roche. Der Autor ist Board Member bzw. Assessor bei QuIP, NordiQC und UKNEQAS sowie Angestellter der Targ | Full Paper |
2020 | Tsai, AG;Glass, DR;Juntilla, M;Hartmann, FJ;Oak, JS;Fernandez-Pol, S;Ohgami, RS;Bendall, SC; | Multiplexed single-cell morphometry for hematopathology diagnostics | Nature medicine | 32161403 | Healthy bone marrow samples from AllCells were ordered, delivered and processed the same day. Healthy bone marrow 1 and 2 refer to the AllCells 9878 and 10874 samples, Save | Full Paper |
2020 | Reyes, M;Filbin, MR;Bhattacharyya, RP;Billman, K;Eisenhaure, T;Hung, DT;Levy, BD;Baron, RM;Blainey, PC;Goldberg, MB;Hacohen, N; | An immune-cell signature of bacterial sepsis | Nature medicine | 32066974 | De-identified BMMC samples were purchased from AllCells or Stemcell Technologies. This study was approved by the Institutional Review Boards at the Broad Institute of MIT and Save | Full Paper |
2020 | Travaglini, KJ;Nabhan, AN;Penland, L;Sinha, R;Gillich, A;Sit, RV;Chang, S;Conley, SD;Mori, Y;Seita, J;Berry, GJ;Shrager, JB;Metzger, RJ;Kuo, CS;Neff, N;Weissman, IL;Quake, SR;Krasnow, MA; | A molecular cell atlas of the human lung from single-cell RNA sequencing | Nature | 33208946 | For bulk RNA-seq of canonical immune populations, whole blood from healthy human donors was obtained commericially (AllCells) in EDTA tubes. Patient tissues were obtained under Save | Full Paper |
2020 | Link, JO;Rhee, MS;Tse, WC;Zheng, J;Somoza, JR;Rowe, W;Begley, R;Chiu, A;Mulato, A;Hansen, D;Singer, E;Tsai, LK;Bam, RA;Chou, CH;Canales, E;Brizgys, G;Zhang, JR;Li, J;Graupe, M;Morganelli, P;Liu, Q;Wu, Q;Halcomb, RL;Saito, RD;Schroeder, SD;Lazerwith, SE;Bondy, S;Jin, D;Hung, M;Novikov, N;Liu, X;Villaseñor, AG;Cannizzaro, CE;Hu, EY;Anderson, RL;Appleby, TC;Lu, B;Mwangi, J;Liclican, A;Niedziela-Majka, A;Papalia, GA;Wong, MH;Leavitt, SA;Xu, Y;Koditek, D;Stepan, GJ;Yu, H;Pagratis, N;Clancy, S;Ahmadyar, S;Cai, TZ;Sellers, S;Wolckenhauer, SA;Ling, J;Callebaut, C;Margot, N;Ram, RR;Liu, YP;Hyland, R;Sinclair, GI;Ruane, PJ;Crofoot, GE;McDonald, CK;Brainard, DM;Lad, L;Swaminathan, S;Sundquist, WI;Sakowicz, R;Chester, AE;Lee, WE;Daar, ES;Yant, SR;Cihlar, T; | Clinical targeting of HIV capsid protein with a long-acting small molecule | Nature | 32612233 | Human peripheral blood mononuclear cells (PBMCs) were collected from healthy volunteers under informed consent; their use was approved by an institutional review board at AllCells Save | Full Paper |
2020 | Ferrari, S;Jacob, A;Beretta, S;Unali, G;Albano, L;Vavassori, V;Cittaro, D;Lazarevic, D;Brombin, C;Cugnata, F;Kajaste-Rudnitski, A;Merelli, I;Genovese, P;Naldini, L; | Efficient gene editing of human long-term hematopoietic stem cells validated by clonal tracking | Nature biotechnology | 32601433 | (Biovision) and 50 nM UM171 (STEMCell Technologies), unless otherwise specified. G-CSF mPB CD34+ HSPCs were purified with the CliniMACS CD34 Reagent System (Miltenyi Biotec) from Mobilized Leukopak (AllCells) upon approval by the Ospedale San Raffae | Full Paper |
2020 | Spindler, MJ;Nelson, AL;Wagner, EK;Oppermans, N;Bridgeman, JS;Heather, JM;Adler, AS;Asensio, MA;Edgar, RC;Lim, YW;Meyer, EH;Hawkins, RE;Cobbold, M;Johnson, DS; | Massively parallel interrogation and mining of natively paired human TCRαβ repertoires | Nature biotechnology | 32393905 | novel ACT. Online Methods Sourcing and Processing Human Materials De-identified peripheral blood mononuclear cells (PBMCs) in leukopaks were obtained from HLA-A*02:01 and HLA-A*24:02 healthy donors (AllCells, Alameda, CA, USA), under an Institutiona | Full Paper |
2020 | Dinh, HQ;Eggert, T;Meyer, MA;Zhu, YP;Olingy, CE;Llewellyn, R;Wu, R;Hedrick, CC; | Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone Marrow | Immunity | 32814027 | Human bone marrow cells Fresh bone marrow samples from anonymous healthy adult donors were obtained from AllCells, Inc. (Alameda, CA). For information on donor characteristics, refer to Supplemental Table 1. Upon arrival, the fresh cells were immedia | Full Paper |
2020 | Glass, DR;Tsai, AG;Oliveria, JP;Hartmann, FJ;Kimmey, SC;Calderon, AA;Borges, L;Glass, MC;Wagar, LE;Davis, MM;Bendall, SC; | An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity | Immunity | 32668225 | structive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com ------------------------- | Full Paper |
2020 | Park, HB;Wei, Z;Oh, J;Xu, H;Kim, CS;Wang, R;Wyche, TP;Piizzi, G;Flavell, RA;Crawford, JM; | Sulfamethoxazole drug stress upregulates antioxidant immunomodulatory metabolites in Escherichia coli | Nature microbiology | 32719505 | ndors supply Eurofins DiscoverX with primary human cells: Cell Applications, Inc., CellzDirect, Celsis-IVT, Leukolab, Life Technologies, Lonza, ScienCell, Hemacare Corporation, Stemcell Technologies, AllCells, Physician’s Plasma Alliance, Lifeline Ce | Full Paper |
2020 | Di Genua, C;Valletta, S;Buono, M;Stoilova, B;Sweeney, C;Rodriguez-Meira, A;Grover, A;Drissen, R;Meng, Y;Beveridge, R;Aboukhalil, Z;Karamitros, D;Belderbos, ME;Bystrykh, L;Thongjuea, S;Vyas, P;Nerlov, C; | C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor | Cancer cell | 32330454 | derbos et al., 2017) ------------------------- Biological Samples ------------------------- AEL patient samples (OX1164; AYL050; MKH048; STB115) MDSBio NA Normal adult human bone marrow AllCells NA ------------------------- Critical | Full Paper |
2020 | Li, S;Chen, X;Wang, J;Meydan, C;Glass, JL;Shih, AH;Delwel, R;Levine, RL;Mason, CE;Melnick, AM; | Somatic Mutations Drive Specific, but Reversible, Epigenetic Heterogeneity States in AML | Cancer discovery | 32938585 | from our prior study(9), 5 of which were purchased from AllCells (Alameda, CA, USA) and 9 of which were isolated using magnetic bead positive selection for CD34+ (Miltenyi Biotec) from freshly collected bone marrow samples from individuals without kn | Full Paper |
2020 | Zhou, Y;Han, C;Wang, E;Lorch, AH;Serafin, V;Cho, BK;Gutierrez Diaz, BT;Calvo, J;Fang, C;Khodadadi-Jamayran, A;Tabaglio, T;Marier, C;Kuchmiy, A;Sun, L;Yacu, G;Filip, SK;Jin, Q;Takahashi, YH;Amici, DR;Rendleman, EJ;Rawat, R;Bresolin, S;Paganin, M;Zhang, C;Li, H;Kandela, I;Politanska, Y;Abdala-Valencia, H;Mendillo, ML;Zhu, P;Palhais, B;Van Vlierberghe, P;Taghon, T;Aifantis, I;Goo, YA;Guccione, E;Heguy, A;Tsirigos, A;Wee, KB;Mishra, RK;Pflumio, F;Accordi, B;Basso, G;Ntziachristos, P; | Posttranslational Regulation of the Exon Skipping Machinery Controls Aberrant Splicing in Leukemia | Cancer discovery | 32444465 | Our study provides a new proof-of-principle for posttranslational regulation of splicing factors independently of mutations in aggressive T-cell leukemia. It further suggests a new drug Save | Full Paper |
2020 | Tochigi, T;Miyamoto, T;Hatakeyama, K;Sakoda, T;Ishihara, D;Irifune, H;Shima, T;Kato, K;Maeda, T;Ito, T;Handa, H;Akashi, K;Kikushige, Y; | Aromatase is a novel neosubstrate of cereblon responsible for immunomodulatory drug-induced thrombocytopenia | Blood | 32219443 | Human adult bone marrow and granulocyte colony-stimulating factor-mobilized peripheral blood samples were obtained from healthy donors or purchased from AllCells. Cord blood Save | Full Paper |
2020 | Montalban Bravo, G;Kanagal-Shamanna, R;Darbaniyan, F;Ganan-Gomez, I;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, K;Chien, K;Bueso-Ramos, C;Kantarjian, H;Garcia-Manero, G; | Genomic and Transcriptomic Differences of Myelodysplastic Syndrome/Myeloproliferative Neoplasm with Ring Sideroblasts and Thrombocytosis (MDS/MPN-RS-T) and Myelodysplastic Syndrome with Ring Sideroblasts (MDS-RS) | Blood | CD34+ cells from bone marrow samples of 4 pts with MDS/MPN-RS-T, 7 pts with MDS-RS and 17 healthy individuals obtained from AllCells (Emeryville, CA) were isolated using the Save | Full Paper | |
2020 | Montalban Bravo, G;Darbaniyan, F;Kanagal-Shamanna, R;Ganan-Gomez, I;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, K;Chien, K;Bueso-Ramos, C;Kantarjian, H;Garcia-Manero, G; | Transcriptomic Features and Deregulation of Genes Involved in Monopoiesis Influence Outcomes of Patients with Chronic Myelomonocytic Leukemia | Blood | INTRODUCTION: Chronic myelomonocytic leukemia (CMML) is characterized by TET2, SRSF2, ASXL1 and RAS pathway mutations known to induce myelomonocytic bias. We have previously shown that upregulation of KDM6B, a histone demethylase that acts as an inna | Full Paper | |
2020 | Walters, M;Chui, D;Farrell, J;Lal, A;Locatelli, F;Kwiatkowski, J;Porter, J;Sauer, M;Thuret, I;Hongeng, S;Kulozik, A;Thrasher, A;Yannaki, E;Yang, J;Whitney, D;Petrusich, A;Colvin, R;Thompson, A; | Response of Patients with Transfusion-Dependent β-Thalassemia (TDT) to Betibeglogene Autotemcel (beti-cel; LentiGlobin for β-Thalassemia) Gene Therapy Based on HBB Genotype and Disease Genetic Modifiers | Blood | Introduction We investigated the impact of β-thalassemia genotypes and disease genetic modifiers including HBA and KLF1 genotype and sentinel single-nucleotide polymorphism (SNP) genotypes at 3 major HbF quantitative trait loci (QTL) o | Full Paper | |
2020 | Kanter, J;Tisdale, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Chen, R;Gallagher, M;Ding, Y;Goyal, S;Paramore, C;Thompson, A;Walters, M; | Improvements in Health-Related Quality of Life for Patients Treated with LentiGlobin for Sickle Cell Disease (bb1111) Gene Therapy | Blood | Background In patients with sickle cell disease (SCD), health-related quality of life (HRQoL) is worse than in the general population and comparable or worse than in patients with other chronic or painful diseases such as cystic fibros | Full Paper | |
2020 | Thompson, A;Kwiatkowski, J;Porter, J;Hongeng, S;Yannaki, E;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Guo, R;Liu, W;Colvin, R;Walters, M;Locatelli, F; | Favorable Outcomes in Pediatric Patients in the Phase 3 Hgb-207 (Northstar-2) and Hgb-212 (Northstar-3) Studies of Betibeglogene Autotemcel Gene Therapy for the Treatment of Transfusion-Dependent β-Thalassemia | Blood | Introduction Betibeglogene autotemcel (beti-cel; LentiGlobin for β-thalassemia) gene therapy is being evaluated for the treatment of transfusion-dependent β-thalassemia (TDT). Initial positive results of beti-cel in the phase 3 studies | Full Paper | |
2020 | Kwiatkowski, J;Walters, M;Hongeng, S;Locatelli, F;Rasko, J;Cavazzana, M;Chen, Y;Colvin, R;Thompson, A; | Long-Term Efficacy and Safety of Betibeglogene Autotemcel Gene Therapy for the Treatment of Transfusion-Dependent β-Thalassemia: Results in Patients with up to 6 Years of Follow-up | Blood | Introduction The goal of betibeglogene autotemcel (beti-cel; LentiGlobin for β-thalassemia) gene therapy in patients with transfusion-dependent β-thalassemia (TDT) is lifelong, stable production of functional adult hemoglobin (Hb) suff | Full Paper | |
2020 | Thompson, A;Walters, M;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Schmidt, M;DelCarpini, J;Pierciey, F;Miller, A;Gallagher, M;Chen, R;Goyal, S;Kanter, J;Tisdale, J; | Resolution of Serious Vaso-Occlusive Pain Crises and Reduction in Patient-Reported Pain Intensity: Results from the Ongoing Phase 1/2 HGB-206 Group C Study of LentiGlobin for Sickle Cell Disease (bb1111) Gene Therapy | Blood | Background Sickle cell disease (SCD) is caused by abnormal sickle hemoglobin (HbS) and results in chronic hemolytic anemia, painful vaso-occlusive events (VOEs), and progressive vasculopathy that lead to significant morbidity. While ac | Full Paper | |
2020 | Rothe, K;Babaian, A;Nakamichi, N;Chen, M;Chafe, SC;Watanabe, A;Forrest, DL;Mager, DL;Eaves, CJ;Dedhar, S;Jiang, X; | Integrin-Linked Kinase Mediates Therapeutic Resistance of Quiescent CML Stem Cells to Tyrosine Kinase Inhibitors | Cell stem cell | 32413332 | Full Paper | |
2020 | Nakao, S;Arai, Y;Tasaki, M;Yamashita, M;Murakami, R;Kawase, T;Amino, N;Nakatake, M;Kurosaki, H;Mori, M;Takeuchi, M;Nakamura, T; | Intratumoral expression of IL-7 and IL-12 using an oncolytic virus increases systemic sensitivity to immune checkpoint blockade | Science translational medicine | 31941828 | hIL-7/hIL-12-VV at MOI 1.0 and cultured for 2 days. Then, the supernatant was filtered through 0.1-μm polyvinylidene difluoride membrane (Millipore) and added to 1 × 105 human PBMCs (AllCells) in a 96-well plate. Seven days later, secretion of human | Full Paper |
2020 | Geng, J;Liu, W;Zhou, H;Zhang, T;Wang, L;Zhang, M;Li, Y;Shen, B;Li, X;Xiao, B; | A Plug-and-Latch Mechanism for Gating the Mechanosensitive Piezo Channel | Neuron | 32142647 | HEK293T Xiao et al., 2011 N/A HEK293T-Piezo1-KO Cahalan et al., 2015 N/A HUVEC Allcells Cat# H-001F-C HeLa Xiao et al., 2011 N/A N2A Coste et al., 2010 N/A C2C12 National Infrastructure of Cell line Source, China Cat# GNM26 | Full Paper |
2020 | Fang, C;Wang, Z;Han, C;Safgren, SL;Helmin, KA;Adelman, ER;Serafin, V;Basso, G;Eagen, KP;Gaspar-Maia, A;Figueroa, ME;Singer, BD;Ratan, A;Ntziachristos, P;Zang, C; | Cancer-specific CTCF binding facilitates oncogenic transcriptional dysregulation | Genome biology | 32933554 | Human CD4 + T cells were purchased from AllCells. Primary human samples were collected with informed consent and analyzed under the supervision of the Institutional Review Board Save | Full Paper |
2020 | Li, M;Liu, W;Bauch, T;Graviss, EA;Arduino, RC;Kimata, JT;Chen, M;Wang, J; | Clearance of HIV infection by selective elimination of host cells capable of producing HIV | Nature communications | 32792548 | pecific-pathogen-free barrier animal facility at the Houston Methodist Research Institute. Newborn male and female mice were injected intrahepatically with CD34+ human stem cells (5 × 104/mouse; AllCells LLC). Three months later, reconstitution of hu | Full Paper |
2020 | Scharenberg, SG;Poletto, E;Lucot, KL;Colella, P;Sheikali, A;Montine, TJ;Porteus, MH;Gomez-Ospina, N; | Engineering monocyte/macrophage-specific glucocerebrosidase expression in human hematopoietic stem cells using genome editing | Nature communications | 32620863 | Human CD34+ HSPCs mobilized from peripheral blood were purchased frozen from AllCells (Almeda, CA, USA) and thawed per manufacturer's instructions. Human Cord blood was Save | Full Paper |
2020 | Wienert, B;Nguyen, DN;Guenther, A;Feng, SJ;Locke, MN;Wyman, SK;Shin, J;Kazane, KR;Gregory, GL;Carter, MAM;Wright, F;Conklin, BR;Marson, A;Richardson, CD;Corn, JE; | Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair | Nature communications | 32355159 | Cryopreserved wildtype human mobilized peripheral blood CD34 + HSPCs from volunteer donors were purchased from Allcells, and were cultured in SFEMII + CC110 (StemCell Save | Full Paper |
2020 | Lareau, CA;Ma, S;Duarte, FM;Buenrostro, JD; | Inference and effects of barcode multiplets in droplet-based single-cell assays | Nature communications | 32054859 | lues have been published, these values were approximated from an examination of a plot previously reported2. Profiling PBMCs using 10× scATAC-seq For 10× scATAC-seq experiments with PBMCs (PB003F, Allcells), frozen cells were quickly thawed in a 37 | Full Paper |
2020 | Mahuron, KM;Moreau, JM;Glasgow, JE;Boda, DP;Pauli, ML;Gouirand, V;Panjabi, L;Grewal, R;Luber, JM;Mathur, AN;Feldman, RM;Shifrut, E;Mehta, P;Lowe, MM;Alvarado, MD;Marson, A;Singer, M;Wells, J;Jupp, R;Daud, AI;Rosenblum, MD; | Layilin augments integrin activation to promote antitumor immunity | The Journal of experimental medicine | 32539073 | Human peripheral blood mononuclear cells (PBMCs) from two individual donors were purchased from AllCells. CD8 + T cells were enriched from these samples using a negative Save | Full Paper |
2020 | Poran, A;Scherer, J;Bushway, ME;Besada, R;Balogh, KN;Wanamaker, A;Williams, RG;Prabhakara, J;Ott, PA;Hu-Lieskovan, S;Khondker, ZS;Gaynor, RB;Rooney, MS;Srinivasan, L; | Combined TCR Repertoire Profiles and Blood Cell Phenotypes Predict Melanoma Patient Response to Personalized Neoantigen Therapy plus Anti-PD-1 | Cell reports. Medicine | 33294862 | Patient PBMCs from NT-001 trial Ott et al., 2020 N/A Healthy Donor PBMCs Precision for Medicine Cat # 93000-10M Healthy Donor PBMCs StemExpress Cat # LE010F Healthy Donor PBMCs AllCells https://www.allcells.com/tissue-products/ | Full Paper |
2020 | Huang, H;Zhu, H;Xie, Q;Tian, X;Yang, X;Feng, F;Jiang, Q;Sheng, X;Yang, Z; | Evaluation of 124I-JS001 for hPD1 immuno-PET imaging using sarcoma cell homografts in humanized mice | Acta pharmaceutica Sinica. B | 32874831 | The human OS-732 cell line was purchased from the Institute of Cancer, Chinese Academy of Medical Sciences (Beijing, China). Normal peripheral blood T cells and CD3+ pan T cells were obtained from AllCells (PB009-1F-C, Emeryville, CA, USA). Phytohema | Full Paper |
2020 | Poran, A;Harjanto, D;Malloy, M;Arieta, CM;Rothenberg, DA;Lenkala, D;van Buuren, MM;Addona, TA;Rooney, MS;Srinivasan, L;Gaynor, RB; | Sequence-based prediction of SARS-CoV-2 vaccine targets using a mass spectrometry-based bioinformatics predictor identifies immunogenic T cell epitopes | Genome medicine | 32791978 | Human PBMCs from HLA-A02:01-positive human donors were isolated using Ficoll separation from apheresis material (AllCells, USA). Twenty-three SARS-CoV-2 peptides predicted to Save | Full Paper |
2020 | Goodwin, M;Lee, E;Lakshmanan, U;Shipp, S;Froessl, L;Barzaghi, F;Passerini, L;Narula, M;Sheikali, A;Lee, CM;Bao, G;Bauer, CS;Miller, HK;Garcia-Lloret, M;Butte, MJ;Bertaina, A;Shah, A;Pavel-Dinu, M;Hendel, A;Porteus, M;Roncarolo, MG;Bacchetta, R; | CRISPR-based gene editing enables FOXP3 gene repair in IPEX patient cells | Science advances | 32494707 | families. Additional healthy donor HSPCs were isolated from umbilical cord blood donors provided by the Binns Program for Cord Blood Research at the Stanford University or purchased commercially from AllCells or StemCell Technologies. For HSPC isolat | Full Paper |
2020 | Grozdanovic, MM;Doyle, CB;Liu, L;Maybruck, BT;Kwatia, MA;Thiyagarajan, N;Acharya, KR;Ackerman, SJ; | Charcot-Leyden crystal protein/galectin-10 interacts with cationic ribonucleases and is required for eosinophil granulogenesis | The Journal of allergy and clinical immunology | 31982451 | Purified human CD34 + cord blood-derived hematopoietic progenitor cells were purchased from AllCells (Emeryville, Calif) and cultured in Iscove modified Dulbecco medium Save | Full Paper |
2020 | Lundin, V;Sugden, WW;Theodore, LN;Sousa, PM;Han, A;Chou, S;Wrighton, PJ;Cox, AG;Ingber, DE;Goessling, W;Daley, GQ;North, TE; | YAP Regulates Hematopoietic Stem Cell Formation in Response to the Biomechanical Forces of Blood Flow | Developmental cell | 32032546 | Cord Blood CD34+ Cells AllCells Cat. CB008F mPB CD34+ Cells AllCells Cat. mPB015F Mouse Embryonic Fibroblasts GlobalStem Cat. 6001G Primary Human Umbilical Vein Endothelial Cells ThermoFisher Cat. C0155C | Full Paper |
2020 | Duncan, RM;Reyes, L;Moats, K;Robinson, RM;Murphy, SA;Kaur, B;Stessman, HAF;Dolloff, NG; | ATF3 Coordinates Antitumor Synergy between Epigenetic Drugs and Protein Disulfide Isomerase Inhibitors | Cancer research | 32561529 | T cells were expanded from human peripheral blood monoleukocytes purchased from AllCells LLC (PB006F). All cells were maintained at 37C and 5% CO 2 . Experiments were Save | Full Paper |
2020 | Yeung, YA;Krishnamoorthy, V;Dettling, D;Sommer, C;Poulsen, K;Ni, I;Pham, A;Chen, W;Liao-Chan, S;Lindquist, K;Chin, SM;Chunyk, AG;Hu, W;Sasu, B;Chaparro-Riggers, J;Djuretic, I; | An Optimized Full-Length FLT3/CD3 Bispecific Antibody Demonstrates Potent Anti-leukemia Activity and Reversible Hematological Toxicity | Molecular therapy : the journal of the American Society of Gene Therapy | 31981494 | After tumor engraftment (day 6-7), animals were administered 2 × 10 7 expanded human T cells (T cells purchased from Allcells; activated according to Miltenyi manufacturing protocol) Save | Full Paper |
2020 | Morgan, RA;Unti, MJ;Aleshe, B;Brown, D;Osborne, KS;Koziol, C;Ayoub, PG;Smith, OB;O'Brien, R;Tam, C;Miyahira, E;Ruiz, M;Quintos, JP;Senadheera, S;Hollis, RP;Kohn, DB; | Improved Titer and Gene Transfer by Lentiviral Vectors Using Novel, Small β-Globin Locus Control Region Elements | Molecular therapy : the journal of the American Society of Gene Therapy | 31628051 | All BM aspirates were obtained from voluntary healthy donors supplied by AllCells (Alameda, CA), which obviated the need for Instituional Review Board review. BM mononuclear cells Save | Full Paper |
2020 | Sinnakannu, JR;Lee, KL;Cheng, S;Li, J;Yu, M;Tan, SP;Ong, CCH;Li, H;Than, H;Anczuków-Camarda, O;Krainer, AR;Roca, X;Rozen, SG;Iqbal, J;Yang, H;Chuah, C;Ong, ST; | SRSF1 mediates cytokine-induced impaired imatinib sensitivity in chronic myeloid leukemia | Leukemia | 32051529 | at the Singapore General Hospital, cord blood samples were obtained from the Singapore Cord Blood Bank, and normal bone marrow cells were obtained commercially from AllCells ( Save | Full Paper |
2020 | Korotchkina, L;Kazyulkin, D;Komarov, PG;Polinsky, A;Andrianova, EL;Joshi, S;Gupta, M;Vujcic, S;Kononov, E;Toshkov, I;Tian, Y;Krasnov, P;Chernov, MV;Veith, J;Antoch, MP;Middlemiss, S;Somers, K;Lock, RB;Norris, MD;Henderson, MJ;Haber, M;Chernova, OB;Gudkov, AV; | OT-82, a novel anticancer drug candidate that targets the strong dependence of hematological malignancies on NAD biosynthesis | Leukemia | 31896781 | Normal human bone marrow mononuclear cells (BM-MNC) were obtained from ALLCELLS, LLC (Almeda CA, USA). Patients’ BM-MNC were procured at the Hematologic Bank of RPCCC and NAMPT monoclonal antibody (clone OMNI 379) was obtained from Cayman Chemicals ( | Full Paper |
2020 | Kravtsova-Ivantsiv, Y;Goldhirsh, G;Ivantsiv, A;Ben Itzhak, O;Kwon, YT;Pikarsky, E;Ciechanover, A; | Excess of the NF-ĸB p50 subunit generated by the ubiquitin ligase KPC1 suppresses tumors via PD-L1- and chemokines-mediated mechanisms | Proceedings of the National Academy of Sciences of the United States of America | 33168738 | Human PBMCs as a source for isolating human NK cells were from AllCells. Mouse anti-CD274 (PD-L1) (29E.2A3) and mouse anti-CD45 (HI30) for mass cytometry were from Bio Save | Full Paper |
2020 | Ojaghi, A;Carrazana, G;Caruso, C;Abbas, A;Myers, DR;Lam, WA;Robles, FE; | Label-free hematology analysis using deep-ultraviolet microscopy | Proceedings of the National Academy of Sciences of the United States of America | 32561645 | bone marrow aspirate was collected from healthy donors (AllCells) and added to Na heparin anticoagulant solution. Smears were made on uncoated quartz slides using 10 μL of whole Save | Full Paper |
2020 | Huang, P;Zhao, Y;Zhong, J;Zhang, X;Liu, Q;Qiu, X;Chen, S;Yan, H;Hillyer, C;Mohandas, N;Pan, X;Xu, X; | Putative regulators for the continuum of erythroid differentiation revealed by single-cell transcriptome of human BM and UCB cells | Proceedings of the National Academy of Sciences of the United States of America | 32457162 | CD34 + stem/progenitor cells (AllCells) were obtained from three mobilized BM and three neonate UCB samples from independent donors. Cells were expanded at 37 C and 5% CO 2 Save | Full Paper |
2020 | Belling, JN;Heidenreich, LK;Tian, Z;Mendoza, AM;Chiou, TT;Gong, Y;Chen, NY;Young, TD;Wattanatorn, N;Park, JH;Scarabelli, L;Chiang, N;Takahashi, J;Young, SG;Stieg, AZ;De Oliveira, S;Huang, TJ;Weiss, PS;Jonas, SJ; | Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cells | Proceedings of the National Academy of Sciences of the United States of America | 32358194 | Mixed donor umbilical cord CD34+ Hematopoietic stem and progenitor cells (Allcells Inc.) were thawed and prestimulated as described by Hoban et al. (44). Postacoustofluidic treatment, HSPCs were cultured in X-VIVO 15 supplemented with 50 ng/μL recomb | Full Paper |
2020 | Borodovsky, A;Barbon, CM;Wang, Y;Ye, M;Prickett, L;Chandra, D;Shaw, J;Deng, N;Sachsenmeier, K;Clarke, JD;Linghu, B;Brown, GA;Brown, J;Congreve, M;Cheng, RK;Dore, AS;Hurrell, E;Shao, W;Woessner, R;Reimer, C;Drew, L;Fawell, S;Schuller, AG;Mele, DA; | Small molecule AZD4635 inhibitor of A2AR signaling rescues immune cell function including CD103+ dendritic cells enhancing anti-tumor immunity | Journal for immunotherapy of cancer | 32727810 | 01 individuals. As previously described,38 naïve CD8+ T cells were co-cultured with autologous monocyte-derived DCs, both cell populations isolated from PBMC from HLA-A*0201 positive healthy donors (AllCells, Research Blood Components). Monocytes wer | Full Paper |
2020 | Kotanides, H;Li, Y;Malabunga, M;Carpenito, C;Eastman, SW;Shen, Y;Wang, G;Inigo, I;Surguladze, D;Pennello, AL;Persaud, K;Hindi, S;Topper, M;Chen, X;Zhang, Y;Bulaon, DK;Bailey, T;Lao, Y;Han, B;Torgerson, S;Chin, D;Sonyi, A;Haidar, JN;Novosiadly, RD;Moxham, CM;Plowman, GD;Ludwig, DL;Kalos, M; | Bispecific Targeting of PD-1 and PD-L1 Enhances T-cell Activation and Antitumor Immunity | Cancer immunology research | 32873605 | Monocytes were isolated from frozen normal peripheral blood mononuclear cells (PBMC) purchased from AllCells (PB005F) using Monocyte Isolation Kit (Miltenyi Biotec; 130-091-153). Immature DCs were generated by culturing the monocytes in RPMI1640 medi | Full Paper |
2020 | Liu, Y;Lv, S;Liu, D;Song, F; | Recent development of amorphous metal coordination polymers for cancer therapy | Acta biomaterialia | 32942012 | e) IC 50 values obtained from cytotoxicity assays against Jurkat human ALLcells after incubating with free MTX, Gd-MTX, [email protected]/DOPE, and [email protected]/DOPE-AA. f) Save | Full Paper |
2020 | Fujita, H;Sasaki, T;Miyamoto, T;Akutsu, SN;Sato, S;Mori, T;Nakabayashi, K;Hata, K;Suzuki, H;Kosaki, K;Matsuura, S;Matsubara, Y;Amagai, M;Kubo, A; | Premature aging syndrome showing random chromosome number instabilities with CDC20 mutation | Aging cell | 33094908 | An age-matched normal control bone marrow mononuclear cell sample was obtained from AllCells. Cells were cultured in MethoCult H4034 Optimum (STEMCELL Technologies) at 1 × Save | Full Paper |
2020 | Yao, X;Ding, J; | Effects of Microstripe Geometry on Guided Cell Migration | ACS applied materials & interfaces | 32479054 | Primary rat mesenchymal stem cells (rMSCs) were purchased from Shanghai Allcells Tech. (China) and cultured in minimum essential medium α (MEM α, Gibco). Mouse embryo fibroblast cell line (NIH3T3) and human cervix epithelial carcinoma cell line (Hela | Full Paper |
2020 | Yang, H;Qing, K;Keeler, GD;Yin, L;Mietzsch, M;Ling, C;Hoffman, BE;Agbandje-McKenna, M;Tan, M;Wang, W;Srivastava, A; | Enhanced Transduction of Human Hematopoietic Stem Cells by AAV6 Vectors: Implications in Gene Therapy and Genome Editing | Molecular therapy. Nucleic acids | 32276210 | D, USA) supplemented with 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA) and 1% penicillin-streptomycin (Invitrogen, Grand Island, NY, USA). Human bone marrow CD34+ cells were purchased from AllCells (AllCells Technologies, Emeryville, CA, U | Full Paper |
2020 | Shin, JJ;Schröder, MS;Caiado, F;Wyman, SK;Bray, NL;Bordi, M;Dewitt, MA;Vu, JT;Kim, WT;Hockemeyer, D;Manz, MG;Corn, JE; | Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor Cells | Cell reports | 32877675 | RNA-sequencing datasets This paper BioProject ID PRJNA498122 ------------------------- Experimental Models ------------------------- Mobilized peripheral blood CD34+ Stem/Progenitor cells AllCells mPB015F NOD.Cg-PrkdcscidIl2rgtm1Wjl/Sz | Full Paper |
2020 | Nie, W;Zan, X;Yu, T;Ran, M;Hong, Z;He, Y;Yang, T;Ju, Y;Gao, X; | Synergetic therapy of glioma mediated by a dual delivery system loading α-mangostin and doxorubicin through cell cycle arrest and apoptotic pathways | Cell death & disease | 33116114 | animals The glioma cell lines C6, Gl261 and U87 and human umbilical vein endothelial cells (HUVECs) were purchased from the American Type Culture Collection (ATCC). Primary HUVECs were purchased from ALLCELLS (Shanghai, China). An EGM-2 BulletKit (Lo | Full Paper |
2020 | Zhang, XY;Rajagopalan, D;Chung, TH;Hooi, L;Toh, TB;Tian, JS;Rashid, MBMA;Sahib, NRBM;Gu, M;Lim, JJ;Wang, W;Chng, WJ;Jha, S;Chow, EK; | Frequent upregulation of G9a promotes RelB-dependent proliferation and survival in multiple myeloma | Experimental hematology & oncology | 32477831 | e), 1% Pen Strep (Invitrogen). For adherent cell lines, trypsin (Thermo Scientific, cat# 25200-056) was used to dissociate cells each passage. Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs, AllCells) were cultured in RPMI 1640 medium (BioWe | Full Paper |
2020 | Qian, Z;Fei, J;Zong, S;Yang, K;Li, L;Liu, R;Wang, Z;Cui, Y; | In Situ Visualization and SERS Monitoring of the Interaction between Tumor and Endothelial Cells Using 3D Microfluidic Networks | ACS sensors | 31885254 | Human umbilical vein endothelial cells (HUVEC) were purchased from Shanghai AllCells Biotech. Co., Ltd. Breast cancer cells (MCF7) were purchased from Nanjing KeyGen Biotech. Co., Ltd. The Roswell Park Memorial Institute (RMPI) 1640 cell culture medi | Full Paper |
2020 | Kim, YH;Park, GY;Rabinovitch, N;Tarafder, S;Lee, CH; | Effect of local anesthetics on viability and differentiation of various adult stem/progenitor cells | Stem cell research & therapy | 32894184 | udy that directly compared the effects of LAs across various stem/progenitor cells. Materials and methods Cell isolation Human bone marrow mesenchymal stem/progenitor cells (MSCs) were obtained from AllCells (Alameda, CA). With the Institutional Rev | Full Paper |
2020 | Xiong, MG;Xu, ZS;Li, YH;Wang, SY;Wang, YY;Ran, Y; | RNF152 positively regulates TLR/IL-1R signaling by enhancing MyD88 oligomerization | EMBO reports | 31930677 | HEK293T cells were purchased from ATCC. Human PBMCs were purchased from ALLCELLS. HFFs provided by Prof. Min‐Hua Luo (Wuhan Institute of Virology, CAS) were derived from human foreskins (male) 30. These cells were cultured in DMEM (HyClone) supplemen | Full Paper |
2020 | Han, Y;Gong, T;Zhang, C;Dissanayaka, WL; | HIF-1α Stabilization Enhances Angio-/Vasculogenic Properties of SHED | Journal of dental research | 32298193 | SHED were purchased from AllCells at passage 2 and characterized for mesenchymal origin. The results were published in our previous study (Xu et al. 2017). The cells were cultured in α-Modified Eagle’s Medium supplemented with 10% (v/v) fetal bovine | Full Paper |
2020 | Maser, IP;Hoves, S;Bayer, C;Heidkamp, G;Nimmerjahn, F;Eckmann, J;Ries, CH; | The Tumor Milieu Promotes Functional Human Tumor-Resident Plasmacytoid Dendritic Cells in Humanized Mouse Models | Frontiers in immunology | 33013879 | sulfan (Busilvex ,Pierre Faber) diluted in 0.9% Saline (Braun) was injected intraperitoneally (i.p.). Twenty-four hours later 105 human CD34+ umbilical cord blood-derived HSC (Stem Cell Technologies/Allcells) in 100 μl phosphate-buffered saline (PBS) | Full Paper |
2020 | Ghelani, A;Bates, D;Conner, K;Wu, MZ;Lu, J;Hu, YL;Li, CM;Chaudhry, A;Sohn, SJ; | Defining the Threshold IL-2 Signal Required for Induction of Selective Treg Cell Responses Using Engineered IL-2 Muteins | Frontiers in immunology | 32582190 | CD8+ T cells were purified from PBMCs isolated from healthy human donor derived leukopaks (AllCells) using EasySep CD8+ T cell Negative selection kit (STEMCELL Technologies) Save | Full Paper |
2020 | Shen, S;Sckisel, G;Sahoo, A;Lalani, A;Otter, DD;Pearson, J;DeVoss, J;Cheng, J;Casey, SC;Case, R;Yang, M;Low, R;Daris, M;Fan, B;Agrawal, NJ;Ali, K; | Engineered IL-21 Cytokine Muteins Fused to Anti-PD-1 Antibodies Can Improve CD8+ T Cell Function and Anti-tumor Immunity | Frontiers in immunology | 32457754 | Mismatched donor pair leukopaks were obtained from AllCells Inc., Donor's T cells were isolated using Pan T-cell Isolation Kit (Miltenyi Biotec, # 130-096-535) and a mismatched donor' Save | Full Paper |
2020 | Porcellini, S;Asperti, C;Corna, S;Cicoria, E;Valtolina, V;Stornaiuolo, A;Valentinis, B;Bordignon, C;Traversari, C; | CAR T Cells Redirected to CD44v6 Control Tumor Growth in Lung and Ovary Adenocarcinoma Bearing Mice | Frontiers in immunology | 32117253 | in of the ΔLNGFr (12). Generation of CD44v6 and CD19 CAR T Cells Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats or leukapheresis of healthy donors (San Raffaele Hospital; AllCells) by density gradient centrifugation (Lymph | Full Paper |
2020 | Baslan, T;Kendall, J;Volyanskyy, K;McNamara, K;Cox, H;D'Italia, S;Ambrosio, F;Riggs, M;Rodgers, L;Leotta, A;Song, J;Mao, Y;Wu, J;Shah, R;Gularte-Mérida, R;Chadalavada, K;Nanjangud, G;Varadan, V;Gordon, A;Curtis, C;Krasnitz, A;Dimitrova, N;Harris, L;Wigler, M;Hicks, J; | Novel insights into breast cancer copy number genetic heterogeneity revealed by single-cell genome sequencing | eLife | 32401198 | ta (i.e. ploidy) to achieve absolute copy number values. T-cell and B-cell sample processing and analysis Purified CD8+/CD4+ T-cells and CD19+/CD27+ memory B-cells purified cells were purchased from ALLCELLS (California, USA). Cells were thawed on i | Full Paper |
2020 | Münchow, EA;da Silva, AF;Piva, E;Cuevas-Suárez, CE;de Albuquerque, MTP;Pinal, R;Gregory, RL;Breschi, L;Bottino, MC; | Development of an antibacterial and anti-metalloproteinase dental adhesive for long-lasting resin composite restorations | Journal of materials chemistry. B | 33169763 | Human dental pulp stem cells (hDPSCs, AllCells, LLC, Alameda, CA, USA) obtained from permanent third molars were cultured in low glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Invitrogen Corporation, Grand Island, NY, USA) supplemented with | Full Paper |
2020 | Warnecke, A;Harre, J;Staecker, H;Prenzler, N;Strunk, D;Couillard-Despres, S;Romanelli, P;Hollerweger, J;Lassacher, T;Auer, D;Pachler, K;Wietzorrek, G;Köhl, U;Lenarz, T;Schallmoser, K;Laner-Plamberger, S;Falk, CS;Rohde, E;Gimona, M; | Extracellular vesicles from human multipotent stromal cells protect against hearing loss after noise trauma in vivo | Clinical and translational medicine | 33377658 | Human bone marrow (BM)-derived MSCs for the production of research-grade EV preparations were purchased from AllCells (Alameda, CA). Immunophenotype and viability analysis of Save | Full Paper |
2020 | Pinho, AC;Fonseca, AC;Caseiro, AR;Pedrosa, SS;Amorim, I;Branquinho, MV;Domingos, M;Maurício, AC;Santos, JD;Serra, AC;Coelho, JFJ; | Innovative tailor made dextran based membranes with excellent non-inflammatory response: In vivo assessment | Materials science & engineering. C, Materials for biological applications | 31761159 | Human dental pulp stem cells (hDPSCs) were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01). Minimum Essential Medium (MEM) α, GlutaMAX™ Supplement, no nucleosides, Streptomycin, Amphotericin B and HEPES buffer solution were purchase | Full Paper |
2020 | Wang, J;Ganaie, SS;Cheng, F;Xu, P;Ning, K;Wang, X;Kleiboeker, S;Cheng, S;Qiu, J; | RNA Binding Motif Protein RBM45 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19 through Binding to Novel Intron Splicing Enhancers | mBio | 32156816 | se conditions are dependent on RBM45. MATERIALS AND METHODS Ethics statement. Primary CD34+ hematopoietic stem cells were isolated from bone marrow of healthy human donors. We purchased the cells at AllCells (AllCells LLC, Alameda, CA). All of the c | Full Paper |
2020 | Dubrovsky, L;Ward, A;Choi, SH;Pushkarsky, T;Brichacek, B;Vanpouille, C;Adzhubei, AA;Mukhamedova, N;Sviridov, D;Margolis, L;Jones, RB;Miller, YI;Bukrinsky, M; | Inhibition of HIV Replication by Apolipoprotein A-I Binding Protein Targeting the Lipid Rafts | mBio | 31964734 | PBMCs from donors with the following HLA-B genotypes were purchased from AllCells Inc.: B*35 positive (B*15:17:01/B*35:01:01, B*35:01:01/B*55:01:01, B*35:01:01/B*35:01:01, B*35: Save | Full Paper |
2020 | Han, KH;Arlian, BM;Lin, CW;Jin, HY;Kang, GH;Lee, S;Lee, PC;Lerner, RA; | Agonist Antibody Converts Stem Cells into Migrating Brown Adipocyte-Like Cells in Heart | Cells | 31968623 | one, Chicago, IL, USA) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA). Expi293F cells were cultured in Expi293 Expression Media (Invitrogen, Carlsbad, CA, USA). Human CD34+ cells (AllCells, Alameda, CA, USA) were purchased and rep | Full Paper |
2020 | Zimran, E;Papa, L;Djedaini, M;Patel, A;Iancu-Rubin, C;Hoffman, R; | Expansion and preservation of the functional activity of adult hematopoietic stem cells cultured ex vivo with a histone deacetylase inhibitor | Stem cells translational medicine | 31950644 | Cryopreserved CD34+ cells from mPB or ABM from healthy adult donors were purchased from AllCells (Alameda, California) and stored in liquid nitrogen. The highly purified (90%-98% Save | Full Paper |
2020 | George, MJ;Prabhakara, K;Toledano-Furman, NE;Gill, BS;Wade, CE;Cotton, BA;Cap, AP;Olson, SD;Cox, CS; | Procoagulant in vitro effects of clinical cellular therapeutics in a severely injured trauma population | Stem cells translational medicine | 31903737 | Bone marrow mononuclear cells (BM MNCs) were isolated from fresh whole bone marrow from a commercial source (AllCells, Emeryville, California) according to common protocols Save | Full Paper |
2020 | Hou, L;Voit, RA;Sankaran, VG;Springer, TA;Yuki, K; | CD11c regulates hematopoietic stem and progenitor cells under stress | Blood advances | 33351105 | Human bone marrow cells and whole blood were purchased from AllCells (Quincy, MA). Lineage marker for human hematopoietic system includes: anti-hCD2 (RPA-2.10), anti-hCD3 ( Save | Full Paper |
2020 | Mascarenhas, JO;Rampal, RK;Kosiorek, HE;Bhave, R;Hexner, E;Wang, ES;Gerds, A;Abboud, CN;Kremyanskaya, M;Berenzon, D;Odenike, O;Farnoud, N;Krishnan, A;Weinberg, RS;McGovern, E;Salama, ME;Najfeld, V;Medina-Martinez, JS;Arango Ossa, JE;Levine, MF;Zhou, Y;Sandy, L;Heaney, ML;Levine, RL;Mesa, RA;Dueck, AC;Hoffman, R; | Phase 2 study of ruxolitinib and decitabine in patients with myeloproliferative neoplasm in accelerated and blast phase | Blood advances | 33104796 | Six control plasma samples (3 male, 3 female) collected from healthy donors between the ages of 30 and 50 years were procured from Allcells. Samples from 8 chronic-phase MF Save | Full Paper |
2020 | Marion, W;Boettcher, S;Ruiz-Torres, S;Lummertz da Rocha, E;Lundin, V;Morris, V;Chou, S;Zhao, AM;Kubaczka, C;Aumais, O;Zhang, Y;Shimamura, A;Schlaeger, TM;North, TE;Ebert, BL;Wells, SI;Daley, GQ;Rowe, RG; | An induced pluripotent stem cell model of Fanconi anemia reveals mechanisms of p53-driven progenitor cell differentiation | Blood advances | 33002135 | Institutional Review Board at Boston Children’s Hospital, after informed consent was obtained. This study was performed in accordance with the Declaration of Helsinki. Normal healthy BM mononuclear cells were purchased from AllCells | Full Paper |
2020 | Goldstein, RL;Goyos, A;Li, CM;Deegen, P;Bogner, P;Sternjak, A;Thomas, O;Klinger, M;Wahl, J;Friedrich, M;Rattel, B;Lamas, E;Min, X;Sudom, A;Farshbaf, M;Coxon, A;Balazs, M;Arvedson, T; | AMG 701 induces cytotoxicity of multiple myeloma cells and depletes plasma cells in cynomolgus monkeys | Blood advances | 32886754 | For staining of human PCs, human BM (AllCells) was stained with a fluorochrome-labeled antibody cocktail (supplemental Table 5). For analysis, doublets were gated out, and dead Save | Full Paper |
2020 | Walker, ZJ;VanWyngarden, MJ;Stevens, BM;Abbott, D;Hammes, A;Langouët-Astrie, C;Smith, CA;Palmer, BE;Forsberg, PA;Mark, TM;Jordan, CT;Sherbenou, DW; | Measurement of ex vivo resistance to proteasome inhibitors, IMiDs, and daratumumab during multiple myeloma progression | Blood advances | 32311014 | Normal donor samples were purchased from AllCells. For viability comparison with MM cells in MNC cultures, selection for CD138-positive cells was performed by using magnetic bead Save | Full Paper |
2020 | Romero-Masters, JC;Huebner, SM;Ohashi, M;Bristol, JA;Benner, BE;Barlow, EA;Turk, GL;Nelson, SE;Baiu, DC;Van Sciver, N;Ranheim, EA;Gumperz, J;Sherer, NM;Farrell, PJ;Johannsen, EC;Kenney, SC; | B cells infected with Type 2 Epstein-Barr virus (EBV) have increased NFATc1/NFATc2 activity and enhanced lytic gene expression in comparison to Type 1 EBV infection | PLoS pathogens | 32059024 | ized NSG mice Immunodeficient NSG (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson labs (catalogue number: 005557). Commercially available CD34-depleted human cord blood mononuclear cells (AllCells, LLC) were infected with various amounts o | Full Paper |
2020 | Shao, S;Qin, T;Qian, W;Li, X;Li, W;Han, L;Zhang, D;Wang, Z;Ma, Q;Wu, Z;Wu, E;Lei, J; | Cav-1 Ablation in Pancreatic Stellate Cells Promotes Pancreatic Cancer Growth through Nrf2-Induced shh Signaling | Oxidative medicine and cellular longevity | 32377291 | iated Hospital of Xi'an Jiaotong University, China. 2.3. Cell Lines Aspc-1 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and HUVECs were obtained from ALLCELLS (Emeryville, California, USA). In our exp | Full Paper |
2020 | Liu, H;Cheng, Q;Xu, DS;Wang, W;Fang, Z;Xue, DD;Zheng, Y;Chang, AH;Lei, YJ; | Overexpression of CXCR7 accelerates tumor growth and metastasis of lung cancer cells | Respiratory research | 33129326 | Human umbilical vein endothelial cells (HUVECs) and its completed medium were obtained from AllCells biological technology (Shanghai) co., LTD. And all these cell lines were Save | Full Paper |
2020 | Cheng, B;Ren, Y;Cao, H;Chen, J; | Discovery of novel resorcinol diphenyl ether-based PROTAC-like molecules as dual inhibitors and degraders of PD-L1 | European journal of medicinal chemistry | 32388281 | CD3 single chain variable fragment) and human PD-L1 (hPD-L1). Fresh PBMCs were purchased from ALLCELLS. Centrifuge at 350g for 10 min, discard the supernatant and resuspend in EasySep buffer at a density of 5 x107/mL. CD3+ T cells were isolated from | Full Paper |
2020 | Zhang, D;Qi, BY;Zhu, WW;Huang, X;Wang, XZ; | Crocin alleviates lipopolysaccharide-induced acute respiratory distress syndrome by protecting against glycocalyx damage and suppressing inflammatory signaling pathways | Inflammation research : official journal of the European Histamine Research Society ... [et al.] | 31925528 | HUVECs were cultured in complete culture medium (AllCells, Shanghai, China) in an incubator with a humidified atmosphere of 95% air and 5% CO 2 at 37 C. Cells at 80-90% Save | Full Paper |
2020 | Kuang, Z;Jing, H;Wu, Z;Wang, J;Li, Y;Ni, H;Zhang, P;Wu, W;Wu, M;Zhou, S;Qiu, X;Wu, D;Prinz, B;Baruah, H;Chen, B;Yu, M;Liu, J; | Development and characterization of a novel anti-OX40 antibody for potent immune activation | Cancer immunology, immunotherapy : CII | 32078015 | Two million LoVo cells suspended in 0.2 ml PBS were mixed with 0.66 million human peripheral blood mononuclear cells (PBMCs, ALLCELLS) and co-implanted subcutaneously in the Save | Full Paper |
2020 | Yao, Y;Yang, J;Qin, Q;Tang, C;Li, Z;Chen, L;Li, K;Ren, C;Chen, L;Rao, S; | Functional annotation of genetic associations by transcriptome-wide association analysis provides insights into neutrophil development regulation | Communications biology | 33340029 | Human CD34 + HSPCs from mobilized peripheral blood of deidentified healthy donors were purchased from AllCells. CD34 + HSPCs were cultured in StemSpan SFEM medium ( Save | Full Paper |
2020 | Mendes, LP;Rostamizadeh, K;Gollomp, K;Myerson, JW;Marcos-Contreras, OA;Zamora, M;Luther, E;Brenner, JS;Filipczak, N;Li, X;Torchilin, VP; | Monoclonal antibody 2C5 specifically targets neutrophil extracellular traps | mAbs | 33323006 | Whole blood was purchased from AllCells (AllCells utilizes standardized operating HIPAA compliance and approved protocols are followed by AllCells' collection facilities. The staff Save | Full Paper |
2020 | Zang, L;Song, Y;Yu, F;Liu, X; | Emodin relieved lipopolysaccharide-evoked inflammatory damage in WI-38 cells by up-regulating taurine up-regulated gene 1 | BioFactors (Oxford, England) | 31912578 | The human fibroblasts cell line WI-38 (AllCells, Shanghai, China) was hatched in a mixture of Dulbecco's modified Eagle's medium (DMEM; Gibco, Grand Island, NY), endothelial cell development supplement (Sigma, St. Louis, MO) (60 μg/ml), streptomycin | Full Paper |
2020 | Subhi, H;Husein, A;Mohamad, D;Nurul, A; | Physicochemical, mechanical and cytotoxicity evaluation of chitosan-based accelerated portland cement | Journal of Materials Research and Technology | Stem cells from human exfoliated deciduous teeth (SHED) was purchased from AllCells, USA. The cells were cultured in alpha minimum essential medium [Invitrogen, USA (GIBCO)] Save | Full Paper | |
2020 | Hu, S;Cavanagh, BL;Harrington, R;Ahmad, M;Kearns, G;Meaney, S;Wynne, C; | A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus Patients | International journal of molecular sciences | 33287382 | Microparticles are sub-micron, membrane-bound particles released from virtually allcells and which are present in the circulation. In several autoimmune disorders their amountand Save | Full Paper |
2020 | Wen, W;Song, S;Han, Y;Chen, H;Liu, X;Qian, Q; | An efficient Screening System in Yeast to Select a Hyperactive piggyBac Transposase for Mammalian Applications | International journal of molecular sciences | 32357554 | fied eagle medium (DMEM; Corning, Corning, NY, USA) supplemented with 10% fetal calf serum (FBS, Gibco, USA). The human peripheral blood mononuclear cells (PBMC) of healthy donors were purchased from AllCells (AllCells, Silicon Valley, CA, USA) under | Full Paper |
2020 | Damodharan, SN;Walker, KL;Forsberg, MH;McDowell, KA;Bouchlaka, MN;Drier, DA;Sondel, PM;DeSantes, KB;Capitini, CM; | Analysis of ex vivo expanded and activated clinical-grade human NK cells after cryopreservation | Cytotherapy | 32536506 | All cellular expansions occurred at the WBF under current Good Manufacturing Practice (GMP) quality system guidelines. Fresh leukopacks were commercially obtained (AllCells, Alameda, CA, USA, and Key Biologics, Memphis, TN, USA) from paid human apher | Full Paper |
2020 | Chen, Q;Jia, G;Zhao, X;Bao, Y;Zhang, Y;Ozkan, C;Minev, B;Ma, W; | Novel Survivin Peptides Screened With Computer Algorithm Induce Cytotoxic T Lymphocytes With Higher Cytotoxic Efficiency to Cancer Cells | Frontiers in molecular biosciences | 33102521 | PBMCs from five healthy donors were provided by San Diego Blood Bank and Scripps Green Hospital (San Diego, CA, United States). Normal human umbilical cord blood (UCB) CD34+ cells were purchased from AllCells (Alameda, CA, United States). This study | Full Paper |
2020 | Papa, L;Djedaini, M;Martin, TC;Zangui, M;Beaumont, KG;Sebra, R;Parsons, R;Schaniel, C;Hoffman, R; | Limited Mitochondrial Activity Coupled With Strong Expression of CD34, CD90 and EPCR Determines the Functional Fitness of ex vivo Expanded Human Hematopoietic Stem Cells | Frontiers in cell and developmental biology | 33384995 | Cryopreserved CD34 + cells from G-CSF mobilized peripheral blood (PB) or bone marrow (BM) aspirates harvested from healthy adult donors were purchased from AllCells. Save | Full Paper |
2020 | Yao, X;Xie, L;Zeng, Y; | MiR-9 Promotes Angiogenesis via Targeting on Sphingosine-1- Phosphate Receptor 1 | Frontiers in cell and developmental biology | 32850858 | and S1P3 in those normal ECs and tumor ECs were compared. The relationships between the significant differentially expressed miR-9 loci and specific S1P receptors were analyzed. Cell Culture HUVECs (Allcells, Shanghai, China) were cultured in HUVEC | Full Paper |
2020 | Juillerat, A;Tkach, D;Yang, M;Boyne, A;Valton, J;Poirot, L;Duchateau, P; | Straightforward Generation of Ultrapure Off-the-Shelf Allogeneic CAR-T Cells | Frontiers in bioengineering and biotechnology | 32671047 | ndependent T-cell donors. Significance is determined by a standard unpaired t-test, *p ≤ 0.05, **p ≤ 0.01. Materials and Methods T-Cell Proliferation Cryopreserved human PBMCs were acquired from ALLCELLS (cat #PB006F) and used in accordance with Cel | Full Paper |
2020 | Kuang, Z;Pu, P;Wu, M;Wu, Z;Wang, L;Li, Y;Zhang, S;Jing, H;Wu, W;Chen, B;Liu, J; | A Novel Bispecific Antibody with PD-L1-assisted OX40 Activation for Cancer Treatment | Molecular cancer therapeutics | 32999045 | Monocyte-derived immature dendrite cells (DC) isolated from human PBMCs (ALLCELLS) were maintained in AIM V medium CTS medium supplemented with GM-CSF (10 ng/mL, R&D Save | Full Paper |
2020 | Kotanides, H;Sattler, RM;Lebron, MB;Carpenito, C;Shen, J;Li, J;Surguladze, D;Haidar, JN;Burns, C;Shen, L;Inigo, I;Pennello, AL;Forest, A;Chen, X;Chin, D;Sonyi, A;Topper, M;Boucher, L;Sharma, P;Zhang, Y;Burtrum, D;Novosiadly, RD;Ludwig, DL;Plowman, GD;Kalos, M; | Characterization of 7A5: A Human CD137 (4-1BB) Receptor Binding Monoclonal Antibody with Differential Agonist Properties That Promotes Antitumor Immunity | Molecular cancer therapeutics | 32241872 | Human PBMCs were isolated from whole blood or leukopak obtained from NY Blood Center, AllCells, or Biological Specialty using Ficoll density gradient centrifugation (Ficoll-Paque Save | Full Paper |
2020 | Bian, W;Jing, X;Yang, Z;Shi, Z;Chen, R;Xu, A;Wang, N;Jiang, J;Yang, C;Zhang, D;Li, L;Wang, H;Wang, J;Sun, Y;Zhang, C; | Downregulation of LncRNA NORAD promotes Ox-LDL-induced vascular endothelial cell injury and atherosclerosis | Aging | 32267831 | (Shanghai, China). The C57BL/6 J mice fed with a normal diet were labeled as the WT control group. Cell culture, RNA interference and Adenovirus infection Primary HUVECs were obtained from Shanghai AllCells Biotech Co., Ltd. (Shanghai, China) and cu | Full Paper |
2020 | Karuna, R;Yokokawa, F;Wang, K;Zhang, J;Xu, H;Wang, G;Ding, M;Chan, WL;Abdul Ghafar, N;Leonardi, A;Seh, CC;Seah, PG;Liu, W;Srinivasa, RPS;Lim, SP;Lakshminarayana, SB;Growcott, E;Babu, S;Fenaux, M;Zhong, W;Gu, F;Shi, PY;Blasco, F;Chen, YL; | A Cyclic Phosphoramidate Prodrug of 2'-Deoxy-2'-Fluoro-2'-C-Methylguanosine for the Treatment of Dengue Virus Infection | Antimicrobial agents and chemotherapy | 32958712 | PBMCs (individual donors) were purchased from AllCells (Alameda, CA) or ReachBio (Seattle, WA). Written consent from the donors was available for all samples. All experiments involving human matrices were approved by the Institutional Review Board of | Full Paper |
2020 | Sebrow, J;Goff, SP;Griffin, DO; | Successfully transfected primary peripherally mobilized human CD34+ hematopoietic stem and progenitor cells (HSPCs) demonstrate increased susceptibility to retroviral infection | Virology journal | 32039735 | We obtained 293 T cells and Jurkat cells from ATCC, and peripherally mobilized CD34+ HSPCs from individual donors from AllCells (pre-isolated). 293 T cells were cultured in 20 mm X Save | Full Paper |
2020 | Zhong, C;Yu, Q;Jia, W;Yu, X;Yu, D;Yang, M;Wang, L;Ling, C;Zhu, L; | Mechanism for enhanced transduction of hematopoietic cells by recombinant adeno-associated virus serotype 6 vectors | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 32960474 | Primary human cord blood-derived CD34 + hematopoietic progenitors (mixed, 80% purity) were purchased from AllCells (Alameda, CA, USA) and cultured in C-IMDM in the presence of 1 ng/mL of recombinant human stem cell factor (rhSCF, BD Bioscience, San D | Full Paper |
2020 | Li, L;Wu, Z;Wu, M;Qiu, X;Wu, Y;Kuang, Z;Wang, L;Sun, T;Liu, Y;Yi, S;Jing, H;Zhou, S;Chen, B;Wu, D;Wu, W;Liu, J; | IBI112, a selective anti-IL23p19 monoclonal antibody, displays high efficacy in IL-23-induced psoriasiform dermatitis | International immunopharmacology | 33069927 | Mouse (C57BL/6, Beijing Vital River Laboratory Animal Technology) or cynomolgus monkey splenocytes (AllCells, USA) were cultured in the presence of IL-2 (R&D, USA). Different Save | Full Paper |
2020 | Kaneko, Y;Fukahori, H;Yamagami, K;Kawashima, T;Ito, M;Akamatsu, M;Marui, T;Kato, K;Takahashi, F;Morokata, T; | Effects of AS2819899, a novel selective PI3Kδ inhibitor, in a NZB/W F1 mouse lupus-like nephritis model | International immunopharmacology | 32736191 | Peripheral blood-derived frozen normal pan-T cells (AllCells, Alameda, CA, USA) were thawed according to the manufacturer’s instructions. The cell suspension (1 × 105 cells/well) was mixed with AS2819899 at designated concentrations in the anti-CD3 a | Full Paper |
2020 | Goydel, RS;Weber, J;Peng, H;Qi, J;Soden, J;Freeth, J;Park, H;Rader, C; | Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applications | The Journal of biological chemistry | 32193207 | Thr-245 and Ala-245, which arise from an SNP (rs10820900) in the frizzled domain of human ROR2 (hROR2-Fz). Human PBMCs were purchased from AllCells and cultured in X-VIVO 20 medium (Lonza) with 5% (v/v) off-the-clot human AB serum (Gemini Bio-Product | Full Paper |
2020 | Čuperlović-Culf, M;Khieu, NH;Surendra, A;Hewitt, M;Charlebois, C;Sandhu, JK; | Analysis and Simulation of Glioblastoma Cell Lines-Derived Extracellular Vesicles Metabolome | Metabolites | 32131411 | Normal human astrocytes (NHA, Lonza, Walkersville, MD, USA) were used as normal counterparts and were grown in astrocyte medium-containing supplements (Allcells) and 5% FBS. Save | Full Paper |
2020 | Liu, J;Kozhaya, L;Torres, VJ;Unutmaz, D;Lu, M; | Structure-based discovery of a small-molecule inhibitor of methicillin-resistant Staphylococcus aureus virulence | The Journal of biological chemistry | 32179646 | Blood samples were obtained from healthy, consenting donors as buffy coats (New York Blood Center) and leukopaks (AllCells, Alameda, CA). Human PBMCs were isolated from peripheral blood by density gradient centrifugation using Ficoll-Paque Plus (GE L | Full Paper |
2020 | Thwin, KKM;Ishida, T;Uemura, S;Yamamoto, N;Lin, KS;Tamura, A;Kozaki, A;Saito, A;Kishimoto, K;Mori, T;Hasegawa, D;Kosaka, Y;Nino, N;Takafuji, S;Iijima, K;Nishimura, N; | Level of Seven Neuroblastoma-Associated mRNAs Detected by Droplet Digital PCR Is Associated with Tumor Relapse/Regrowth of High-Risk Neuroblastoma Patients | The Journal of molecular diagnostics : JMD | 31837427 | 1 sample from BioChain (Newark, CA), 2 samples from STEMCELL (Vancouver, Canada), 56 samples from AllCells (Alameda, CA), 16 samples from ReachBio (Seattle, WA), and 28 samples from Loza (Walkersville, MD) (Supplemental Table S1). All PB samples from | Full Paper |
2020 | Xue, WL;Chen, RQ;Zhang, QQ;Li, XH;Cao, L;Li, MY;Li, Y;Lin, G;Chen, Y;Wang, MJ;Zhu, YC; | Hydrogen sulfide rescues high glucose-induced migration dysfunction in HUVECs by upregulating miR-126-3p | American journal of physiology. Cell physiology | 32186933 | Primary human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) (35). These HUVECs were cultured in endothelial complete medium (Allcells, Save | Full Paper |
2020 | Satani, N;Giridhar, K;Cai, C;Wewior, N;Norris, DD;Aronowski, J;Savitz, SI; | Medications for Hypertension Change the Secretome Profile from Marrow Stromal Cells and Peripheral Blood Monocytes | Stem cells international | 32802081 | MSCs were isolated from commercially available fresh human bone marrow aspirates (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously Save | Full Paper |
2020 | Diaz, MF;Horton, PD;Dumbali, SP;Kumar, A;Livingston, M;Skibber, MA;Mohammadalipour, A;Gill, BS;Zhang, S;Cox, CS;Wenzel, PL; | Bone marrow stromal cell therapy improves survival after radiation injury but does not restore endogenous hematopoiesis | Scientific reports | 33335275 | t received wet feed on days 4-30 post-exposure. Culture of human MSCs Human MSCs used for cell therapy were derived from whole bone marrow from independent human donors (commercially obtained from AllCells, LLC, Alameda, CA). Cells were isolated and | Full Paper |
2020 | Dzimianski, JV;Lorig-Roach, N;O'Rourke, SM;Alexander, DL;Kimmey, JM;DuBois, RM; | Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry | Scientific reports | 33303951 | dy at UCSC. All participants agreed to sample banking and future research use. The convalescent seropositive (SP) panel comprised 10 de-identified plasma samples from nine individuals, purchased from AllCells (Alameda, CA, USA). To be eligible for pl | Full Paper |
2020 | Diaz, MF;Horton, PD;Kumar, A;Livingston, M;Mohammadalipour, A;Xue, H;Skibber, MA;Ewere, A;Toledano Furman, NE;Aroom, KR;Zhang, S;Gill, BS;Cox, CS;Wenzel, PL; | Injury intensifies T cell mediated graft-versus-host disease in a humanized model of traumatic brain injury | Scientific reports | 32612177 | Bone marrow stromal cells were derived from whole bone marrow from independent human donors (AllCells, Alameda, CA). Mononuclear cells from whole bone marrow were enriched Save | Full Paper |
2020 | Allen, A;Vaninov, N;Li, M;Nguyen, S;Singh, M;Igo, P;Tilles, AW;O'Rourke, B;Miller, BLK;Parekkadan, B;Barcia, RN; | Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells | Scientific reports | 32576889 | ld times cover the range of expected delivery of the device from cell seeding location to clinical application. PBMC isolation and enrichment Peripheral blood mononuclear cells (PBMCs) obtained from AllCells LLC (AllCells, CA, USA) were freshly isol | Full Paper |
2020 | Siew Ching, H;Thirumulu Ponnuraj, K;Luddin, N;Ab Rahman, I;Nik Abdul Ghani, NR; | Early Odontogenic Differentiation of Dental Pulp Stem Cells Treated with Nanohydroxyapatite-Silica-Glass Ionomer Cement | Polymers | 32957636 | the present study was principally aimed at evaluating the effect of nanoHA-silica-GIC on the differentiation of DPSCs into odontogenic lineage. 2. Materials and Methods 2.1. Cell Culture DPSCs (AllCells, Alameda, CA, USA; Cat no. DP003F) were grown | Full Paper |
2020 | Keskar, V;Sood, A;Loghin, E;Kovacs, E;Duthie, RS;Liu, S;Park, JH;Chadwick, C;Smith, R;Brown, M;Stroncek, DF;Highfill, SL; | Novel DNA-based T-Cell Activator Promotes Rapid T-Cell Activation and Expansion | Journal of immunotherapy (Hagerstown, Md. : 1997) | 32796275 | Activation studies were conducted in 6-well tissue culture plastic plates using commercially available Pan T cells (AllCells, Alameda, CA). Cultures were diluted as needed (1:3 or 1:4) to Save | Full Paper |
2020 | Shrestha, B;Zhang, Y;Yu, B;Li, G;Boucher, JC;Beatty, NJ;Tsai, HC;Wang, X;Mishra, A;Sweet, K;Lancet, JE;Kelley, L;Davila, ML; | Generation of Antitumor T Cells For Adoptive Cell Therapy With Artificial Antigen Presenting Cells | Journal of immunotherapy (Hagerstown, Md. : 1997) | 31834208 | To investigate the ability of aAPCs to support T-cell expansion we isolated T cells from healthy donor PBMCs (ALLcells) using a Human T-cell isolation Kit (Stem Cells Inc., Cambridge, Save | Full Paper |
2020 | Park, LM;Lannigan, J;Jaimes, MC; | OMIP-069: Forty-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood | Cytometry. Part A : the journal of the International Society for Analytical Cytology | 32830910 | All human PBMCs used in this study were obtained from AllCells Alameda. Ethical review and regulatory compliance were conducted by Alpha Independent Review Board under Save | Full Paper |
2020 | Nair, RR;Piktel, D;Hathaway, QA;Rellick, SL;Thomas, P;Saralkar, P;Martin, KH;Geldenhuys, WJ;Hollander, JM;Gibson, LF; | Pyrvinium Pamoate Use in a B cell Acute Lymphoblastic Leukemia Model of the Bone Tumor Microenvironment | Pharmaceutical research | 31989336 | Primary CD3+ T cells, peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) were purchased from AllCells (Allcells.com) and maintained in Lymphocyte Growth Medium-3 (Lonza, Cat No: CC-3211) containing 10% FBS and 1x strep | Full Paper |
2020 | Powell, AB;Ren, Y;Korom, M;Saunders, D;Hanley, PJ;Goldstein, H;Nixon, DF;Bollard, CM;Lynch, RM;Jones, RB;Cruz, CRY; | Engineered Antigen-Specific T Cells Secreting Broadly Neutralizing Antibodies: Combining Innate and Adaptive Immune Response against HIV | Molecular therapy. Methods & clinical development | 33005704 | Peripheral blood samples were obtained from deidentified, healthy donor buffy coats from the NIH, through the Department of Transfusion Medicine, or commercially from AllCells. Save;erved. Peripheral Blood Samples Peripheral blood samples were obt | Full Paper |
2020 | Fang, Y;Zhang, Y;Guo, C;Chen, C;Gao, H;Zhou, X;Liu, T;Qian, Q; | Safety and Efficacy of an Immune Cell-Specific Chimeric Promoter in Regulating Anti-PD-1 Antibody Expression in CAR T Cells | Molecular therapy. Methods & clinical development | 32995356 | PBMCs were purchased from AllCells (USA) using a protocol approved by the Ethics Committee of the Chinese Peoples' Liberation Army General Hospital (S2019-063-01). In total, 5 × Save;Cell Isolation, Electroporation, and Ex Vivo CAR T Cell Expansion | Full Paper |
2020 | Shapiro, J;Iancu, O;Jacobi, AM;McNeill, MS;Turk, R;Rettig, GR;Amit, I;Tovin-Recht, A;Yakhini, Z;Behlke, MA;Hendel, A; | Increasing CRISPR Efficiency and Measuring Its Specificity in HSPCs Using a Clinically Relevant System | Molecular therapy. Methods & clinical development | 32478125 | (5 μL total volume). Complexes were allowed to form for 10-20 min at room temperature before electroporation. Genome Editing by RNP Electroporation in CD34+ HSPCs Mobilized human CD34+ HSPCs (AllCells, Alameda, CA, USA) were thawed and cultured for | Full Paper |
2020 | Morgan, RA;Ma, F;Unti, MJ;Brown, D;Ayoub, PG;Tam, C;Lathrop, L;Aleshe, B;Kurita, R;Nakamura, Y;Senadheera, S;Wong, RL;Hollis, RP;Pellegrini, M;Kohn, DB; | Creating New β-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer Sequences | Molecular therapy. Methods & clinical development | 32426415 | BM CD34+ Cell Culture and Transduction All BM aspirates were obtained from voluntary healthy donors supplied by AllCells (Alameda, CA, USA). BM mononuclear cells were isolated Save | Full Paper |
2020 | Mehler, VJ;Burns, CJ;Stauss, H;Francis, RJ;Moore, ML; | Human iPSC-Derived Neural Crest Stem Cells Exhibit Low Immunogenicity | Molecular therapy. Methods & clinical development | 32055644 | utamine, 2 mM L-glutamine, 50 U/mL penicillin/streptomycin, 20 ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF), and 20 ng/mL IL-4. BM-MSCs were derived from bone marrow aspirates (AllCells, Alameda, CA, USA) in-house as previously des | Full Paper |
2020 | Elnaggar, M;Al-Mohannadi, A;Kizhakayil, D;Raynaud, CM;Al-Mannai, S;Gentilcore, G;Pavlovski, I;Sathappan, A;Van Panhuys, N;Borsotti, C;Follenzi, A;Grivel, JC;Deola, S; | Flow-Cytometry Platform for Intracellular Detection of FVIII in Blood Cells: A New Tool to Assess Gene Therapy Efficiency for Hemophilia A | Molecular therapy. Methods & clinical development | 31886317 | ar endothelial cells) and U937 (pre-monocytic cells) cell lines were kindly provided by Antonia Follenzi (UPO, Italy). HeLa (cervical cancer cells) were purchased from ATCC. PBMCs were purchased from AllCells (Alameda, CA, USA). Cells were cultured i | Full Paper |
2020 | Ning, K;Wang, MJ;Lin, G;Zhang, YL;Li, MY;Yang, BF;Chen, Y;Huang, Y;Li, ZM;Huang, YJ;Zhu, L;Liang, K;Yu, B;Zhu, YZ;Zhu, YC; | eNOS-Nitric Oxide System Contributes to a Novel Antiatherogenic Effect of Leonurine via Inflammation Inhibition and Plaque Stabilization | The Journal of pharmacology and experimental therapeutics | 32238453 | Primary human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (H-001F-C; China) and cultured in HUVEC Growth Medium (H-004; Allcells), and 100 U/ml of Save | Full Paper |
2020 | Zhaorigetu, S;Bair, H;Jin, D;Gupta, VS;Pandit, LM;Bryan, RM;Lally, KP;Olson, SD;Cox, CS;Harting, MT; | Extracellular Vesicles Attenuate Nitrofen-Mediated Human Pulmonary Artery Endothelial Dysfunction: Implications for Congenital Diaphragmatic Hernia | Stem cells and development | 32475301 | Human MSCs were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using density centrifugation and plastic Save | Full Paper |
2020 | Baumgartner, JM;Riviere, P;Lanman, RB;Kelly, KJ;Veerapong, J;Lowy, AM;Kurzrock, R; | Prognostic Utility of Pre- and Postoperative Circulating Tumor DNA Liquid Biopsies in Patients with Peritoneal Metastases | Annals of surgical oncology | 32767050 | Background Circulating tumor DNA (ctDNA) is a promising technology for treatment selection, prognostication, and surveillance after definitive therapy. Its use in the perioperative Save | Full Paper |
2020 | Hamburger, AE;DiAndreth, B;Cui, J;Daris, ME;Munguia, ML;Deshmukh, K;Mock, JY;Asuelime, GE;Lim, ED;Kreke, MR;Tokatlian, T;Kamb, A; | Engineered T cells directed at tumors with defined allelic loss | Molecular immunology | 33012527 | Leukopaks were purchased from AllCells®. Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB), with strict oversight. HIPAA compliance and approved protocols were also followed. Frozen PBMCs were thawed | Full Paper |
2020 | Xu, H;Hamburger, AE;Mock, JY;Wang, X;Martin, AD;Tokatlian, T;Oh, J;Daris, ME;Negri, KR;Gabrelow, GB;Wu, ML;Nampe, DP;Asuelime, GE;McElvain, ME;Sandberg, ML;Kamb, A; | Structure-function relationships of chimeric antigen receptors in acute T cell responses to antigen | Molecular immunology | 32768859 | Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols Save | Full Paper |
2020 | Wang, Z;Zhou, G;Risu, N;Fu, J;Zou, Y;Tang, J;Li, L;Liu, H;Liu, Q;Zhu, X; | Lenalidomide Enhances CAR-T Cell Activity Against Solid Tumor Cells | Cell transplantation | 32967454 | line U251 CD133-OE luc. Similarly, the tumor cell line U251 WT luc, MDA-MB-453 luc, and MDA-MB-468 luc were also obtained by nucleofection. Peripheral blood mononuclear cells (PBMCs) were bought from ALLCELLS (PB005F, Alameda, CA, USA). Following the | Full Paper |
2020 | Zhao, C;Zhao, Z;Wang, Z;Hu, L;Wang, H;Fang, Z; | Supervillin promotes tumor angiogenesis in liver cancer | Oncology reports | 32468064 | Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells LLC (cat. H-004; AllCells LLC) in a 0.25% gelatin-coated culture flask. HepG2, Bel7405 and MHCC- Save | Full Paper |
2020 | Lee, KS;Lee, J;Lee, P;Kim, CU;Kim, DJ;Jeong, YJ;Park, YJ;Tesh, VL;Lee, MS; | Exosomes released from Shiga toxin 2a-treated human macrophages modulate inflammatory responses and induce cell death in toxin receptor expressing human cells | Cellular microbiology | 32772454 | Human CD14 + monocytes were purchased from AllCells LLC and maintained in RPMI 1640 medium containing 10% FBS, 5.0 μg/ml streptomycin and 5.0 U/ml penicillin. Cells (1.0 × Save | Full Paper |
2020 | Monroe, MN;Zhaorigetu, S;Gupta, VS;Jin, D;Givan, KD;Curylo, AL;Olson, SD;Cox, CS;Segura, A;Buja, LM;Grande-Allen, KJ;Harting, MT; | Extracellular vesicles influence the pulmonary arterial extracellular matrix in congenital diaphragmatic hernia | Pediatric pulmonology | 32568428 | Human mesenchymal stromal cells (hMSCs) were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using Save | Full Paper |
2020 | Zaw, SYM;Kaneko, T;Zaw, ZCT;Sone, PP;Murano, H;Gu, B;Okada, Y;Han, P;Katsube, KI;Okiji, T; | Crosstalk between dental pulp stem cells and endothelial cells augments angiogenic factor expression | Oral diseases | 32248596 | Stem cells from human exfoliated deciduous teeth (SHEDs; AllCells) were cultured with a stem cell growth medium (Dulbecco's modified Eagle's medium (DMEM)/F12; Gibco) containing 15% fetal bovine serum (Gibco-BRL, Grand Island, NY), 10,000 units penic | Full Paper |
2020 | Huang, GL;Nampe, DP;Yi, J;Gabrelow, GB;Negri, KR;Kamb, A;Xu, H; | A multivariate, quantitative assay that disentangles key kinetic parameters of primary human T cell function in vitro | PloS one | 33166305 | Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols Save | Full Paper |
2020 | Wang, X;Chacon, LI;Derakhshandeh, R;Rodriguez, HJ;Han, DD;Kostyushev, DS;Henry, TD;Traverse, JH;Moyé, L;Simari, RD;Taylor, DA;Springer, ML; | Impaired therapeutic efficacy of bone marrow cells from post-myocardial infarction patients in the TIME and LateTIME clinical trials | PloS one | 32841277 | Healthy de-identified donor BM MNCs were purchased from AllCells (Alameda, CA). These MNCs were manually isolated at AllCells using Ficoll conditions like that in the Sepax Save | Full Paper |
2020 | Schönherz, AA;Bødker, JS;Schmitz, A;Brøndum, RF;Jakobsen, LH;Roug, AS;Severinsen, MT;El-Galaly, TC;Jensen, P;Johnsen, HE;Bøgsted, M;Dybkær, K; | Normal myeloid progenitor cell subset-associated gene signatures for acute myeloid leukaemia subtyping with prognostic impact | PloS one | 32324791 | [18] GSE42519, [19] GSE19599, [20] GSE17054, [21] and GSE19429 [22] cohorts. For GSE63270, [18] and GSE17054, [21] human bone marrow mononuclear cells (BMMCs) from healthy donors were purchased from ALLCELLS (Emeryville, CA) where collection protocol | Full Paper |
2020 | Tani, J;Ito, Y;Tatemichi, S;Yamakami, M;Fukui, T;Hatano, Y;Kakimoto, S;Kotani, A;Sugimura, A;Mihara, K;Yamamoto, R;Tanaka, N;Minami, K;Takahashi, K;Hirato, T; | Physicochemical and biological evaluation of JR-131 as a biosimilar to a long-acting erythropoiesis-stimulating agent darbepoetin alfa | PloS one | 32302352 | fects of the test substances on colony-forming unit-erythroid (CFU-E)- and burst-forming unit-erythroid (BFU-E)-derived colony formation in human bone-marrow-derived mononuclear cells (purchased from AllCells, CA) were examined. The cells were obtain | Full Paper |
2020 | Steinberg, L; | The Effects of P. Gingivalis Ceramide Lipids on Platelet Size, Agglutination, Aggregation, and Vascular Surface Adhesion Markers | Thesis | Background: Increased bleeding on probing has been proven to be correlated with the progression of periodontal disease. Gingival bleeding in sites with periodontitis has often been attributed to chronic inflammation. Previous research has documented | Full Paper | |
2020 | Kim, DH;Kim, DH;Heck, BE;Shaffer, M;Yoo, KH;Hur, J; | PPAR-δ agonist affects adipo-chondrogenic differentiation of human mesenchymal stem cells through the expression of PPAR-γ | Regenerative therapy | 33426208 | In this experimental study, frozen human bone marrow mononuclear cells were used for isolation (AllCells, Alameda, CA, USA) [25]. Cells were resuspended in α-minimum essential Save;mation condition and its correlation with PPAR-δ. 2 Methods 2.1 Is | Full Paper |
2020 | Zhang, E;Xie, L;Qin, P;Lu, L;Xu, Y;Gao, W;Wang, L;Xie, MH;Jiang, W;Liu, S; | Quality by Design-Based Assessment for Analytical Similarity of Adalimumab Biosimilar HLX03 to Humira® | The AAPS journal | 32385732 | PMBC cells (Allcells) were incubated with mAbs with a 2-fold serial dilution for 30 min followed by TNFα-Biotin (Sinobio) incubation. After labeled by Streptavidin-PE (eBioscience), Save | Full Paper |
2020 | Dai, R;Heid, B;Xu, X;Xie, H;Reilly, CM;Ahmed, SA; | EGR2 is elevated and positively regulates inflammatory IFNγ production in lupus CD4+ T cells | BMC immunology | 32646370 | ethods Human peripheral blood mononuclear cells (PBMCs) The PBMCs of human patients of lupus (n = 4, all female) and healthy controls (n = 4, 2 male and 2 female) were purchased directly from AllCells LLC (Alameda, CA, USA). Based on the information | Full Paper |
2020 | Dong, W;Zhang, X;Lu, Z; | Effect of 532nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor in cultured human vascular endothelial cells | Photodiagnosis and photodynamic therapy | 32344194 | Working drug solutions (0-8 μg/ml) were prepared by diluting the stock solution with RPMI-1640 medium (AllCells). A 532 nm neodymium-doped yttrium orthovanadate (Nd:YVO 4 ) Save | Full Paper |
2020 | Pieles, O;Reck, A;Reichert, TE;Morsczeck, C; | p53 inhibits the osteogenic differentiation but does not induce senescence in human dental follicle cells | Differentiation; research in biological diversity | 32473528 | Human dental follicle cells (DFCs) were obtained from AllCells. DFCs were cultivated as described previously (Morsczeck et al., 2016). Cells were analyzed before and after the Save | Full Paper |
2020 | Lafleur, MA;Werner, J;Fort, M;Lobenhofer, EK;Balazs, M;Goyos, A; | MRGPRX2 activation as a rapid, high-throughput mechanistic-based approach for detecting peptide-mediated human mast cell degranulation liabilities | Journal of immunotoxicology | 32525431 | Human mobilized peripheral blood CD34+ cells (derived from healthy donors treated with G-CSF to mobilize CD34+ progenitor cells from the bone marrow to the peripheral blood, then isolated using anti-CD34 magnetic beads) (#mPB016F, AllCells, Alameda, | Full Paper |
2020 | Yu, Z;Yin, S;Zhang, W;Jiang, X;Hu, J; | Picosecond laser texturing on titanium alloy for biomedical implants in cell proliferation and vascularization | Journal of biomedical materials research. Part B, Applied biomaterials | 31692202 | Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCells Biological Technology Limited company and cultured at 1 × 105 on plates with Dulbecco's Modified Eagle's medium supplemented with 10% fetal bovine serum, 100 U/ml streptomyc | Full Paper |
2020 | Bhargav, A;Min, KS;Wen Feng, L;Fuh, JYH;Rosa, V; | Taguchi's methods to optimize the properties and bioactivity of 3D printed polycaprolactone/mineral trioxide aggregate scaffold: Theoretical predictions and experimental validation | Journal of biomedical materials research. Part B, Applied biomaterials | 31112004 | The use of human dental pulp stem cells (DPSC) from single donor (DPF003, Allcells, Alameda) was approved by NUS Institutional Review Board (Approval Number: NUS 2094). The characterization and expressions of CD34, CD73, CD90, CD105 are available els | Full Paper |
2020 | Al-Amoodi, AS;Sakashita, K;Ali, AJ;Zhou, R;Lee, JM;Tehseen, M;Li, M;Belmonte, JCI;Kusakabe, T;Merzaban, JS; | Using Eukaryotic Expression Systems to Generate Human α1,3-Fucosyltransferases That Effectively Create Selectin-Binding Glycans on Stem Cells | Biochemistry | 32901486 | leukemia cell line), THP1 (human acute monocytic leukemia), HL60 (human acute promyelocytic leukemia) (ATCC), and MSCs (primary mesenchymal stromal cells) (AllCells) were Save | Full Paper |
2020 | Behbehani, GK;Finck, R;Samusik, N;Sridhar, K;Fantl, WJ;Greenberg, PL;Nolan, GP; | Profiling myelodysplastic syndromes by mass cytometry demonstrates abnormal progenitor cell phenotype and differentiation | Cytometry. Part B, Clinical cytometry | 31917512 | in Table S2, with the risk‐based clinical status of MDS patients indicated by IPSS category and morphologic characteristics (Greenberg et al., 1997). Five healthy control samples were obtained from Allcells, Inc. using the same protocol. Normal bone | Full Paper |
2020 | Kim, DH;Kim, DH;Heck, BE;Shaffer, M;Hur, J;Yoo, KH; | A natural supplement formula reduces anti-oxidative stress and enhances osteo-chondrogenic differentiation potential in mesenchymal stem cells | Journal of clinical biochemistry and nutrition | 32523247 | rozen mononuclear cells from human bone marrow were purchased (AllCells, Emeryville, CA) and used to isolate human bone marrow-mesenchymal stem cells (hBM-MSCs). Briefly, thawed mononuclear cells were cultured in Dulbecco’s modified Eagle medium (DME | Full Paper |
2020 | Wang, P;Qin, W;Liu, T;Jiang, D;Cui, L;Liu, X;Fang, Y;Tang, X;Jin, H;Qian, Q; | PiggyBac-engineered T cells expressing a glypican-3-specific chimeric antigen receptor show potent activities against hepatocellular carcinoma | Immunobiology | 31522780 | Human peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from AllCells (AllCells, Silicon, USA). 5 × 106−1 × 107 PBMCs were electroporated with 6 μg of GPC3CAR plasmid or an equal quantity of MOCK plasmid in an electroporat | Full Paper |
2020 | Liu, H;Gemmell, L;Lin, R;Zuo, F;Balfour, HH;Woo, JC;Hayes, GM; | Development of an Improved Epstein-Barr Virus (EBV) Neutralizing Antibody Assay to Facilitate Development of a Prophylactic gp350-Subunit EBV Vaccine | Mediterranean journal of hematology and infectious diseases | 32180911 | Healthy human donor serum samples were purchased from AllCells, LLC and Bioreclamation, LLC (n=39). Samples were heated at 56 oC for 30 minutes to inactivate complement, and Save | Full Paper |
2020 | Chien, KS;Class, CA;Montalban-Bravo, G;Wei, Y;Sasaki, K;Naqvi, K;Ganan-Gomez, I;Yang, H;Soltysiak, KA;Kanagal-Shamanna, R;Do, KA;Kantarjian, HM;Garcia-Manero, G; | LILRB4 expression in chronic myelomonocytic leukemia and myelodysplastic syndrome based on response to hypomethylating agents | Leukemia & lymphoma | 32036728 | BM samples from healthy individuals were obtained from AllCells (Emeryville, CA, USA). CD34+ cells were isolated using the CD34 MicroBead Kit (Miltenyi Biotec, Bergisch Gladback, Save | Full Paper |
2020 | Wu, T; | The mean ± 1 SD was calculated for triplicate cultures. The laboratory (ReachBio LLC, Seattle, Washington) was blinded to the identify of the test compounds and docetaxel served as the positive control, with blank and vehicle as negative controls. | Thesis | The surviving mice of the “young adult” group at day 35 post-TBI were sacrificed and 5×104 BM cells acquired from femurs were plated in ColonyGEL Mouse Complete Medium (Reach Bio, Seattle, WA) as previously described [131, 135]. After 7 days of incub | Full Paper | |
2020 | White, CM;Haidekker, MA;Kisaalita, WS; | Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D Cultures | SLAS technology | 31997709 | Human bone marrow derived (Tulane Center for Gene Therapy) 3.2 ± 1.4 AFM indentation Spread morphology on glass coverslips; cytoplasm measured; spherical AFM tip 76 Human bone marrow derived (AllCells) 33 ± 7 AFM indentation Spread morphology on Ther | Full Paper |
2020 | Chen, Y; | NOVEL SIGNALS IN STRESS ERYTHROPOIESIS: COORDINATED CONTROL OF WNT, PGE2 AND PERK SIGNALING PATHWAYS REGULATES EXPANSION AND DIFFERENTIATION OF STRESS ERYTHROID PROGENITORS | Thesis | Human bone marrow mononuclear cells (MNCs) (ReachBio) were thawed at 37°C water according to the ReachBio instructions. MNCs were enumerated and cell concentrations were adjusted to 5 105 cells/mL. MNCs were cultured in Gibco RPMI-1640 (Invitrogen | Full Paper | |
2020 | Wu, JL;McIntyre, PW;Hong, JM;Yassen, GH;Bruzzaniti, A; | Effects of radiopaque double antibiotic pastes on the proliferation, alkaline phosphatase activity and mineral deposition of dental pulp stem cells | Archives of oral biology | 32485262 | Human DPSC were purchased from ALLCells (Alameda, CA, USA), passaged once and aliquots stored in liquid nitrogen. For each experiment, DPSC were thawed and seeded into 10-cm2 dishes and cultured in α-MEM (HyClone Laboratories Inc., South Logan, UT, U | Full Paper |
2020 | Madanagopal, TT;Franco-Obregón, A;Rosa, V; | Comparative study of xeno-free induction protocols for neural differentiation of human dental pulp stem cells in vitro | Archives of oral biology | 31600663 | The DPSC were isolated from the third molar from single donor (DPF003, AllCells, USA). Due to ethical requirements, the age, gender and ethnic group of the donor cannot be disclosed Save | Full Paper |
2020 | Pieles, O;Reck, A;Morsczeck, C; | High endogenous expression of parathyroid hormone-related protein (PTHrP) supports osteogenic differentiation in human dental follicle cells | Histochemistry and cell biology | 32710187 | Human dental follicle cells (DFCs), which were purchased from AllCells, were cultivated in DMEM (Dulbecco's modified Eagle medium) supplemented with 10% fetal bovine serum and Save | Full Paper |
2020 | Fujishiro, A;Iwasa, M;Fujii, S;Maekawa, T;Andoh, A;Tohyama, K;Takaori-Kondo, A;Miura, Y; | Menatetrenone facilitates hematopoietic cell generation in a manner that is dependent on human bone marrow mesenchymal stromal/stem cells | International journal of hematology | 32572826 | Normal human BM samples were purchased from AllCells (Alameda, CA). To confirm consistency among donors, multiple batches of BM-MSCs were isolated as described previously [ Save | Full Paper |
2020 | Yu, CI;Marches, F;Wu, TC;Martinek, J;Palucka, K; | Techniques for the generation of humanized mouse models for immuno-oncology | Methods in enzymology | 32178826 | Adult CD34 + HPCs from G-CSF mobilized peripheral blood or bone marrow may be obtained from Allcells (Alameda, CA, USA) or Lonza (Basel, Switzerland). Cord blood CD34 + Save | Full Paper |
2020 | Enezei, H;Qabbani, A;Ahmad, A;Khamis, M;Hassani, A;Hamad, H; | The Effect of Strontium on Osteoblastogenesis and Osteoclastogenesis in Dental Stem Cells-induced Epidermal Growth Factor at Molecular Level: In Vitro Study | Journal of Hard Tissue Biology | AllCells, LLC Company, Emeryville, CA, USA Dental Stem Cells (DSCs) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Dulbecco’s modified Eagles medium (DMEM) high glucose(4.5g/l) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Fetal bovine ser | Full Paper | |
2020 | Kuang, Z;Li, L;Zhang, P;Chen, B;Wu, M;Ni, H;Yi, S;Zou, J;Liu, J; | A novel antibody targeting TIM-3 resulting in receptor internalization for cancer immunotherapy | Antibody therapeutics | 33928230 | The antibody was purified in-house (Innovent Biologics Co., Ltd., Suzhou, China) from HEK293 cells with either transient or stable expression unless indicated otherwise. Cell culture Human PBMCs (AllCells, Alameda, CA, USA) were cultured in AIM V M | Full Paper |
2020 | Moussi, K;Abusamra, DB;Yassine, O;Merzaban, J;Kosel, J; | Strain-induced Differentiation of Mesenchymal Stem Cells | Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference | 33018453 | Human bone marrow derived mesenchymal stem/stromal cells (hMSCs) were purchased from AllCells. All the experiments were performed using MSCs in their 3-5th passage. An Save | Full Paper |
2020 | Yang, Z;Wang, T;Wu, D;Min, Z;Tan, J;Yu, B; | RNA N6-methyladenosine reader IGF2BP3 regulates cell cycle and angiogenesis in colon cancer | Journal of experimental & clinical cancer research : CR | 32993738 | lls were cultured in RPMI 1640 medium supplemented with 10% FBS (Invitrogen, Carlsbad, CA, USA) at 37 °C in a 5% CO2 atmosphere. Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured in End | Full Paper |
2020 | MacGlashan, D; | Modulating the Human Basophil Phenotype During Its Development and Maturation: Basophils Derived from In Vitro Cultures of CD34+ Progenitor Cells | Methods in molecular biology (Clifton, N.J.) | 32766967 | CD34+ progenitor stems (commercially prepared, multiple sources, e.g., AllCells (Quincy, MA, USA), or Fred Hutchinson Cancer Research Center (Seattle, WA, USA)). 12. FBS (fetal bovine serum) (multiple sources, heat-inactivated). 13. Penicillin/stre | Full Paper |
2020 | Urkasemsin, G;Rungarunlert, S;Ferreira, JN; | Bioprinting Strategies for Secretory Epithelial Organoids | Methods in molecular biology (Clifton, N.J.) | 32207117 | hDPSC lines are commercially available through AllCells (Alameda, CA, USA) and should be used at lower passage to guarantee that their proliferation rate and phenotype are stable. 2. The same lot of FBS should be utilized during the all experiments. | Full Paper |
2020 | Han, SS;Kang, SW; | Metabolic Labeling of Live Stem Cell for In Vitro Imaging and In Vivo Tracking | Methods in molecular biology (Clifton, N.J.) | 30997638 | Stem cell therapy offers promising solutions to diseases and injuries that traditional medicines and therapies can't effectively cure. To get and explain their full therapeutic potentials, the Save | Full Paper |
2020 | Al-Mohannadi, A;Deola, S;Malki, A; | Visualization of Factor Viii with Flow-Cytometry as a tool for Novel Gene Therapy Approach in Hemophilia A | University of the Future: Re-Imagining Research and Higher Education | received as a kind gift from Antonia Follenzi, Health Sciences Department in Piemonte Orientale University in Novara-Italy. HELA cells (cervical cancer) were purchased from American Type Culture Collection (ATCC™), USA. PBMCs purchased by AllCells (C | Full Paper | |
2020 | Romito, M;Juillerat, A;Kok, Y;HIldenbeutel, M;Rhiel, M;Andrieux, G;Geiger, J;Rudolph, C;Mussolino, C;Duclert, A;Metzner, K;Duchateau, P;Cathomen, T;Cornu, T; | Clinically relevant CCR5 editing in primary CD4+ T cells with TALEN confers resistance to HIV-1 infection | Authorea, Inc. | For oligonucleotide capture assay (OCA), human PBMCs (ALLCELLS, USA) were plated at a density of 1x106 cells/ml in X-vivo-15 media (Lonza, Switzerland) supplemented with 5% Save | Full Paper | |
2020 | Turchiano, G;Andrieux, G;Blattner, G;Pennucci, V;Klermund, J;Monaco, G;Poddar, S;Mussolino, C;Cornu, T;Boerries, M;Cathomen, T; | Quantitative Evaluation of Chromosomal Rearrangements in Primary Gene-Edited Human Stem Cells by Preclinical CAST-Seq | SSRN Electronic Journal | Cell culture and transfection. Cryopreserved human CD34+ HSPCs derived from cord blood (AllCells, Cat.# CB008F; StemCell-Technologies, Cat.# 70008.3) were thawed and cultivated in a density range of 0.5-1 x 106 /ml at 37°C, 5% CO2 in GMP-grade CellG | Full Paper | |
2020 | Lu, B;Yang, H;Liu, F;Sun, Y;He, H;Ding, X;Hu, S;xia, l; | Escherichia coli Nissle 1917 secreting functional interleukin 2 targets tumours and enhances the immune response to suppress tumours | Research Square | CT26 colon carcinoma lines were maintained by our laboratory, and peripheral blood mononuclear cells (PBMCs) were purchased from Allcells Biotechnology (Shanghai) Co., Ltd. Save | Full Paper | |
2020 | Zhang, Y;Wang, P;wang, t;Fang, Y;Ding, Y;Qian, Q; | Chimeric Antigen Receptor T Cells Engineered to Secrete CD40 Agonist Antibodies Enhance Antitumor Efficacy | Research Square | Human peripheral blood mononuclear cells (PBMC) from healthy donors were purchased from AllCells (Shanghai, China) and cryopreserved in our laboratory. Save | Full Paper | |
2020 | Mangiras, D;Mattheakis, P;Ribet, P;Dimitrakopoulos, G; | Soft-Clustering Driven Flip-flop Placement Targeting Clock-induced OCV | Proceedings of the 2020 International Symposium on Physical Design | In this way, the cluster initialization of clock net n is done on AllCells that includes both the cells connected to n and the pre-defined cluster centers of the hierarchically lower clock sub- Save | Full Paper | |
2020 | Li, W;Yang, S;Xu, P;Zhang, D;Tong, Y;Chen, L;Jia, B;Li, A;Ru, D;Zhang, B;Liu, M;Lian, C;Chen, C;Fu, W;Yuan, S;Ren, X;Liang, Y;Yang, Z;Li, W;Wang, S;Zhang, X;Lu, H;Xu, J;Wang, H;Yu, W; | Human Identical Sequences of SARS-CoV-2 Promote Clinical Progression of COVID-19 by Upregulating Hyaluronan via NamiRNA-Enhancer Network | bioRxiv | HUVEC (human umbilical vein endothelial cell, bought from AllCells) was cultured in the commercial culture medium (AllCells, H-004) according to the manufacturer's guidelines. Save | Full Paper | |
2020 | Fiskin, E;Lareau, C;Eraslan, G;Ludwig, L;Regev, A; | Single-cell multimodal profiling of proteins and chromatin accessibility using PHAGE-ATAC | bioRxiv | For PBMC and CD8 T cell experiments, cryopreserved PBMCs or CD8 T cells (AllCells) were thawed, washed in PBS and resuspended in cold Flow cytometry buffer (FC buffer; PBS containing 2% FBS). All centrifugation steps were carried out at 350g, 4min, 4 | Full Paper | |
2020 | Mimitou, E;Lareau, C;Chen, K;Zorzetto-Fernandes, A;Takeshima, Y;Luo, W;Huang, T;Yeung, B;Thakore, P;Wing, J;Nazor, K;Sakaguchi, S;Ludwig, L;Sankaran, V;Regev, A;Smibert, P; | Scalable, multimodal profiling of chromatin accessibility and protein levels in single cells | bioRxiv | Cryopreserved healthy donor peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BM) were obtained from AllCells (USA) or Cellular Technology Limited (CTL) and processed immediately after thawing. NIH-3T3 and HEK293FT cells were maintain | Full Paper | |
2020 | Steinbuck, M;Seenappa, L;Jakubowski, A;McNeil, L;Haqq, C;DeMuth, P; | A Lymph Node Targeted Amphiphile Vaccine Induces Potent Cellular and Humoral Immunity to SARS-CoV-2 | bioRxiv | SARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and ALLCELLS (Alameda, CA), respectively. All samples were received and stored frozen at -80°C until analysis | Full Paper | |
2020 | Keating, S;Mizrahi, R;Adams, M;Asensio, M;Benzie, E;Carter, K;Chiang, Y;Edgar, R;Gautam, B;Gras, A;Leong, J;Leong, R;Lim, Y;Manickam, V;Medina-Cucurella, A;Niedecken, A;Saini, J;Simons, J;Spindler, M;Stadtmiller, K;Tinsley, B;Wagner, E;Wayham, N;Tracy, L;Lundberg, C;Büscher, D;Terencio, J;Roalfe, L;Pearce, E;Richardson, H;Goldblatt, D;Ramjag, A;Carrington, C;Simmons, G;Muench, M;Chamow, S;Monroe, B;Olson, C;Oguin, T;Lynch, H;Jeanfreau, R;Meyer, E;Adler, A;Johnson, D; | Capturing and Recreating Diverse Antibody Repertoires as Multivalent Recombinant Polyclonal Antibody Drugs | bioRxiv | Plasma-derived polyclonal antibodies are polyvalent drugs used for many important clinical indications that require modulation of multiple drug targets simultaneously, including Save | Full Paper | |
2020 | Sharma, R;Dever, D;Lee, C;Azizi, A;Pan, Y;Camarena, J;Köhnke, T;Bao, G;Porteus, M;Majeti, R; | TRACE-Seq Reveals Clonal Reconstitution Dynamics of Gene Targeted Human Hematopoietic Stem Cells | bioRxiv | For in vivo studies presented, cord blood-derived CD34 + cells were purchased from AllCells or Stemcell Technologies and were thawed according to the manufacturer's Save | Full Paper | |
2020 | Miller, I;Sun, L;Harris, A;Gamboa, L;Zamat, A;Kwong, G; | Remote control of CAR T cell therapies by thermal targeting | bioRxiv | Primary Human CD3+ cells were obtained from an anonymous donor blood after apheresis (AllCells) and were cryopreserved in 90% FBS and 10% DMSO until subsequent use. After Save | Full Paper | |
2020 | Mandal, S;Sunagawa, S;Prathipati, P;Belshan, M;Shibata, A;Destache, C; | Targeted immuno-antiretroviral HIV therapeutic approach to provide dual protection and boosts cellular immunity: A proof-of-concept study | bioRxiv | Human immunodeficiency virus (HIV)-infected active and latent CCR5 expressing long-lived T-cells are the primary barrier to HIV/AIDS eradication. Broadly neutralizing antibodies and Save | Full Paper | |
2020 | Burley, D;Campagna, C;Harte, A;Kelly, S;Spiegelhoff, M; | Effects of Erythrocyte Aggregation on Blood Rheology in Regard to Future Sepsis Diagnosis Application | Thesis | The preparation of the blood dextran solutions for the micro-ESR, capillary fill, and vibration syllectometry were all done as one big batch. Blood was obtained from AllCells, LLC, a reputable blood and marrow cell supplier from Quincy, Massachusetts | Full Paper | |
2020 | Fleischer, LC; | Engineering Novel Chimeric Antigen Receptors (CARs) for T-cell Malignancies Using Innate Immune Cells | Thesis | Methods and Materials γδ T-cell expansion. gd T cells were expanded from cryopreserved PBMCs purchased from AllCells (Alameda, California) or from fresh PBMCs isolated from 30-50 mL consented, healthy adult blood. PBMCs were isolated from blood using | Full Paper | |
2020 | O'Brien, G; | Genetic and epigenetic consequences of radiation exposure in human and mouse leukaemogenesis | Thesis | Greece with informed consent from each patient. Bone marrow aspirates were obtained from 5 normal donors (ALLCELLS, Alameda, CA) with informed consent and ethical approval from the Alpha Independent Review Board. Bone marrow aspirates from 7 AML pati | Full Paper | |
2020 | Kimmey, SC; | Mapping Single Cell Protein & Biosynthesis Dynamics Across Human Embryonic & Adult Stem Cell Specification | Thesis | Fresh bone marrow aspirates from two independent donors were obtained from AllCells (Alameda, CA) on the same day of collection, and immediately after receiving was placed in 37C 5% CO2 incubator for 30 minutes prior to SOM3B labeling. A mixture of a | Full Paper | |
2020 | Davis-Marcisak, E;Fitzgerald, A;Kessler, M;Danilova, L;Jaffee, E;Zaidi, N;Weiner, L;Fertig, E; | Transfer learning between preclinical models and human tumors identifies conserved NK cell activation signature in anti-CTLA-4 responsive tumors | bioRxiv | Fresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selected from donor peripheral blood using CD56 positivity. Donor NK cell purity Save | Full Paper | |
2020 | Burgenson, D; | Development of a Cell-Free Protein Expression System Derived from Human Blood Cells | Thesis | A comparison between methods used to produce leukapheresis products purchased from Allcells and the New York Blood Center. From the experiments performed, the Allcells Save | Full Paper | |
2020 | Yun, ZX; | Identification of G9a as a Therapeutically Targetable Driver of Multiple Myeloma Tumorigenesis | Thesis | dissociate cells each passage. Cryopreserved Peripheral Blood Mononuclear Cells (PBMC, AllCells) were cultured in RPMI 1640 medium (BioWest, Kansas City, MO) with supplement of 10% FBS (HyClone) and 1% penicillin/streptomycin (Invitrogen) one day bef | Full Paper | |
2020 | Lindgren, A;Mohidin, D; | Applying Digital Marketing Methods in the Healthcare Industry: A Case Study at Immuneed | Thesis | Immuneed Volume Immuneed Traffic Celentyx Volume Celentyx Traffic Reachbio Volume Reachbio Traffic Eurofins Volume Eurofins Traffic Immundnz Volume Immundnz Traffic Nexelis Volume Nexelis Traffic ImmuneCarta Volume ImmuneCarta Traffic | Full Paper | |
2020 | Su, R;Dong, L;Li, Y;Gao, M;Han, L;Wunderlich, M;Deng, X;Li, H;Huang, Y;Gao, L;Li, C;Zhao, Z;Robinson, S;Tan, B;Qing, Y;Qin, X;Prince, E;Xie, J;Qin, H;Li, W;Shen, C;Sun, J;Kulkarni, P;Weng, H;Huang, H;Chen, Z;Zhang, B;Wu, X;Olsen, MJ;Müschen, M;Marcucci, G;Salgia, R;Li, L;Fathi, AT;Li, Z;Mulloy, JC;Wei, M;Horne, D;Chen, J; | Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion | Cancer cell | 32531268 | After 7 days of selection with 0.5mg/ml G418 Sulfate (10131027, Thermo Fisher Scientific) in ColonyGEL (1201, ReachBio Research Lab), the cells were collected and injected into lethally irradiated (960 rads) 8- to 10-week-old B6.SJL (CD45.1, RRID: IM | Full Paper |
2020 | Young, TM;Reyes, C;Pasnikowski, E;Castanaro, C;Wong, C;Decker, CE;Chiu, J;Song, H;Wei, Y;Bai, Y;Zambrowicz, B;Thurston, G;Daly, C; | Autophagy protects tumors from T cell-mediated cytotoxicity via inhibition of TNFα-induced apoptosis | Science immunology | 33443027 | For human tumor cell killing experiments using CD3 bispecific antibody, human T cells were isolated from peripheral blood mononuclear cells (PBMCs) (ReachBio) using Dynabeads Untouched Human T Cells Kit (Thermo Fisher Scientific) | Full Paper |
2020 | Shen, C;Sheng, Y;Zhu, AC;Robinson, S;Jiang, X;Dong, L;Chen, H;Su, R;Yin, Z;Li, W;Deng, X;Chen, Y;Hu, YC;Weng, H;Huang, H;Prince, E;Cogle, CR;Sun, M;Zhang, B;Chen, CW;Marcucci, G;He, C;Qian, Z;Chen, J; | RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid Leukemia | Cell stem cell | 32402250 | Thereafter, the transduced cells were then plated into ColonyGEL methylcellulose medium (ReachBio, Seattle, WA) supplied with 10 ng/ml of murine recombinant IL-3, IL-6, GM-CSF and 30 ng/ml of murine recombinant SCF, along with 1.0 mg/ml of G418 (Gibc | Full Paper |
2020 | Chiu, D;Tavaré, R;Haber, L;Aina, OH;Vazzana, K;Ram, P;Danton, M;Finney, J;Jalal, S;Krueger, P;Giurleo, JT;Ma, D;Smith, E;Thurston, G;Kirshner, JR;Crawford, A; | A PSMA-Targeting CD3 Bispecific Antibody Induces Antitumor Responses that Are Enhanced by 4-1BB Costimulation | Cancer immunology research | 32184296 | PBMCs were obtained from ReachBio (WA) for in vivo (ReachBio). C4-2 tumor cells were subcutaneously injected with 5 × 10 6 tumor cells and 1 × 10 6 human PBMCs (ReachBio). Save | Full Paper |
2020 | Patterson, AM;Liu, L;Sampson, CH;Plett, PA;Li, H;Singh, P;Mohammad, KS;Hoggatt, J;Capitano, ML;Orschell, CM;Pelus, LM; | A Single Radioprotective Dose of Prostaglandin E2 Blocks Irradiation-Induced Apoptotic Signaling and Early Cycling of Hematopoietic Stem Cells | Stem cell reports | 32735825 | CFCs were quantitated as described by Hoggatt et al. (2013a). TNCs from non-IR mice were plated at 2 × 104 per 35-mm dish in triplicate in ColonyGEL Mouse Complete Medium (Reach Bio, Seattle, WA). Cells from IR mice were plated at 0.5-1 × 106 per dis | Full Paper |
2020 | Paulson, RF;Hariharan, S;Little, JA; | Stress erythropoiesis: definitions and models for its study | Experimental hematology | 32750404 | Control PBMCs were purchased from ReachBio. PBMCs obtained were plated at 1 x 10 6 cells/ml stress erythropoiesis differentiation media containing human growth factors. The cells Save | Full Paper |
2020 | Patterson, AM;Plett, PA;Chua, HL;Sampson, CH;Fisher, A;Feng, H;Unthank, JL;Miller, SJ;Katz, BP;MacVittie, TJ;Orschell, CM; | Development of a Model of the Acute and Delayed Effects of High Dose Radiation Exposure in Jackson Diversity Outbred Mice; Comparison to Inbred C57BL/6 Mice | Health physics | 32932286 | Colony-forming unit cell (CFU-C) analyses Functional BM progenitor number was analyzed utilizing methylcellulose colony assays as per manufacturer instructions (Colony Gel 1202, ReachBio, Seattle, WA) | Full Paper |
2020 | Radtke, S;Colonna, L;Perez, AM;Hoffman, M;Kean, LS;Kiem, HP; | Isolation of a Highly Purified HSC-enriched CD34+CD90+CD45RA- Cell Subset for Allogeneic Transplantation in the Nonhuman Primate Large-animal Model | Transplantation direct | 33134503 | CFC) assays were sorted in purity mode to prevent crosscontamination by different subsets. Colony-forming Cell Assay For CFC assays, 1000 to 1200 sorted cells were seeded into 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA). Hematopoietic colonies wer | Full Paper |
2019 | Bonner, M;Kanter, J;Macari, E;Lane, R;Lewis, G;Coles, P;Kassenaar, S;Mynampati, S;Schulze, R;Hebert, M;Walters, M;Thompson, A;Asmal, M;Tisdale, J;Pierciey, F; | The Relationships between Target Gene Transduction, Engraftment of HSCs and RBC Physiology in Sickle Cell Disease Gene Therapy | Blood | Background LentiGlobin for Sickle Cell Disease (SCD) gene therapy contains ex-vivo lentiviral vector (LVV)-mediated addition of a modified β-globin gene (βA-T87Q) into autologous CD34+ hematopoietic stem cells (HSCs). The safety and ef | Full Paper | |
2019 | Kanter, J;Tisdale, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Schmidt, M;Miller, A;Pierciey, F;Huang, W;Ribeil, J;Thompson, A;Walters, M; | Resolution of Sickle Cell Disease Manifestations in Patients Treated with Lentiglobin Gene Therapy: Updated Results from the Phase 1/2 Hgb-206 Group C Study | Blood | Background β-globin gene transfer into hematopoietic stem cells (HSCs) could reduce or eliminate sickle cell disease (SCD)-related manifestations. LentiGlobin for SCD gene therapy contains autologous CD34+ cells transduced with the BB3 | Full Paper | |
2019 | Walters, M;Tisdale, J;Kwiatkowski, J;Krishnamurti, L;Mapara, M;Schmidt, M;Miller, A;Pierciey, F;Huang, W;Ribeil, J;Kanter, J;Thompson, A; | Exploring the Drivers of Potential Clinical Benefit in Initial Patients Treated in the Hgb-206 Study of Lentiglobin for Sickle Cell Disease (SCD) Gene Therapy | Blood | Background LentiGlobin for SCD gene therapy contains autologous CD34+ hematopoietic stem cells (HSCs) transduced with the BB305 lentiviral vector (LVV) encoding β-globin with an anti-sickling substitution (T87Q). Its safety and efficac | Full Paper | |
2019 | Eapen, M;Neuberg, D;Mendizabal, A;Stevenson, K;Antin, J;DiFronzo, N;El Rassi, F;Lulla, P;Waller, E;Garrison, J;Smith, S;Sullivan, K;Walters, M;Krishnamurti, L; | A Phase II Trial to Compare Allogeneic Transplant Vs. Standard of Care for Severe Sickle Cell Disease: Blood and Marrow Transplant Clinical Trials Network (BMT CTN) Protocol 1503 | Blood | Background: Allogeneic hematopoietic cell transplantation (HCT) is a curative treatment for sickle cell disease (SCD). A pilot trial confirmed the suitability of a myeloablative conditioning regimen (busulfan/fludarabine/r-ATG) for HLA-matched siblin | Full Paper | |
2019 | Loi, S;Giobbie-Hurder, A;Gombos, A;Bachelot, T;Hui, R;Curigliano, G;Campone, M;Biganzoli, L;Bonnefoi, H;Jerusalem, G;Bartsch, R;Rabaglio-Poretti, M;Kammler, R;Maibach, R;Smyth, M;Di Leo, A;Colleoni, M;Viale, G;Regan, M;André, F;Fumagalli, D;Gelber, R;Goulioti, T;Hiltbrunner, A;Hui, R;Roschitzki, H;Ruepp, B;Boyle, F;Stahel, R;Aebi, S;Coates, A;Goldhirsch, A;Karlsson, P;Kössler, I;Fournarakou, S;Gasca, A;Pfister, R;Ribeli-Hofmann, S;Weber, M;Celotto, D;Comune, C;Frapolli, M;Sánchez-Hohl, M;Huang, H;Mahoney, C;Price, K;Scott, K;Shaw, H;Fischer, S;Greco, M;King, C;Andrighetto, S;Piccart-Gebhart, M;Findlay, H;Jenkins, M;Karantza, V;Mejia, J;Schneier, P; | Pembrolizumab plus trastuzumab in trastuzumab-resistant, advanced, HER2-positive breast cancer (PANACEA): a single-arm, multicentre, phase 1b–2 trial | The Lancet Oncology | PD-L1 status for the first 79 patients was assessed with the QualTek immunohistochemistry assay (QualtTek Molecular Laboratories, Santa Barbara, CA, USA) in which PD-L1 staining in at least 1% of tumour cells or any staining in stroma were defined as | Full Paper | |
2019 | Kassim, A;Walters, M;Eapen, M;Allison, B;Mendizabal, A;Brodsky, R;DeBaun, M; | Reduced Intensity Conditioning for Haploidentical Bone Marrow Transplantation in Patients with Symptomatic Sickle Cell Disease: BMT CTN Protocol 1507 | Blood | Background: Hematopoietic cell transplantation (HCT) has curative potential in sickle cell disease (SCD). A reduced intensity haploidentical bone marrow transplant (haplo-BMT) platform piloted by investigators at Johns Hopkins, addressed key barriers | Full Paper | |
2019 | Nghiem, P;Bhatia, S;Lipson, EJ;Sharfman, WH;Kudchadkar, RR;Brohl, AS;Friedlander, PA;Daud, A;Kluger, HM;Reddy, SA;Boulmay, BC;Riker, AI;Burgess, MA;Hanks, BA;Olencki, T;Margolin, K;Lundgren, LM;Soni, A;Ramchurren, N;Church, C;Park, SY;Shinohara, MM;Salim, B;Taube, JM;Bird, SR;Ibrahim, N;Fling, SP;Homet Moreno, B;Sharon, E;Cheever, MA;Topalian, SL; | Durable Tumor Regression and Overall Survival in Patients With Advanced Merkel Cell Carcinoma Receiving Pembrolizumab as First-Line Therapy | J. Clin. Oncol. | 30726175 | 21,22. PD-L1 IHC. PD-L1 staining (anti-PD-L1 clone 22C3; Merck Research Laboratories, Kenilworth, NJ) was performed at QualTek Molecular Laboratories (Newtown, PA) on formalin-fixed paraffin-embedded sections from pretreatment MCC biopsies, as previo | Full Paper |
2019 | Fedoriw, A;Rajapurkar, SR;O'Brien, S;Gerhart, SV;Mitchell, LH;Adams, ND;Rioux, N;Lingaraj, T;Ribich, SA;Pappalardi, MB;Shah, N;Laraio, J;Liu, Y;Butticello, M;Carpenter, CL;Creasy, C;Korenchuk, S;McCabe, MT;McHugh, CF;Nagarajan, R;Wagner, C;Zappacosta, F;Annan, R;Concha, NO;Thomas, RA;Hart, TK;Smith, JJ;Copeland, RA;Moyer, MP;Campbell, J;Stickland, K;Mills, J;Jacques-O'Hagan, S;Allain, C;Johnston, D;Raimondi, A;Porter Scott, M;Waters, N;Swinger, K;Boriack-Sjodin, A;Riera, T;Shapiro, G;Chesworth, R;Prinjha, RK;Kruger, RG;Barbash, O;Mohammad, HP; | Anti-tumor Activity of the Type I PRMT Inhibitor, GSK3368715, Synergizes with PRMT5 Inhibition through MTAP Loss | Cancer Cell | 31257072 | GSK3368715 was evaluated at 20, 5, 1.25, 0.3125 and 0.078 mM in a total of 10 patient samples. DLBCL patient cells from 10 unique donors were received as frozen samples from Conversant Bio. The human biological samples were sourced ethically and thei | Full Paper |
2019 | Leighl, NB;Hellmann, MD;Hui, R;Carcereny, E;Felip, E;Ahn, MJ;Eder, JP;Balmanoukian, AS;Aggarwal, C;Horn, L;Patnaik, A;Gubens, M;Ramalingam, SS;Lubiniecki, GM;Zhang, J;Piperdi, B;Garon, EB; | Pembrolizumab in patients with advanced non-small-cell lung cancer (KEYNOTE-001): 3-year results from an open-label, phase 1 study | Lancet Respir Med | 30876831 | NR=not reached. PD-L1=programmed death ligand 1. TPS=tumour proportion score. *PD-L1 TPS 1% on the basis of the QualTek assay (Molecular Laboratories, Goleta | Full Paper |
2019 | Melamed, Z;López-Erauskin, J;Baughn, MW;Zhang, O;Drenner, K;Sun, Y;Freyermuth, F;McMahon, MA;Beccari, MS;Artates, JW;Ohkubo, T;Rodriguez, M;Lin, N;Wu, D;Bennett, CF;Rigo, F;Da Cruz, S;Ravits, J;Lagier-Tourenne, C;Cleveland, DW; | Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegeneration | Nat. Neurosci. | 30643298 | ... titute for the kind contribution of the SIN18 vector. We thank B. Ren for providing the Illumina sequencing platform. We thank A. Goginashvili for valued input on the manuscript. We thank J. Nuovo at Phylogeny Inc. (Columbus, OH) for performing t | Full Paper |
2019 | Thompson, A;Walters, M;Kwiatkowski, J;Hongeng, S;Porter, J;Sauer, M;Thrasher, A;Thuret, I;Elliot, H;Tao, G;Colvin, R;Locatelli, F; | Northstar-2: Updated Safety and Efficacy Analysis of Lentiglobin Gene Therapy in Patients with Transfusion-Dependent β-Thalassemia and Non-β0/β0 Genotypes | Blood | Background Transfusion-dependent β-thalassemia (TDT) is treated with regular, lifelong red blood cell (RBC) transfusions and despite iron-chelating therapy, carries a risk of serious organ damage from iron overload and other complicati | Full Paper | |
2019 | Petermann, F;Pękowska, A;Johnson, CA;Jankovic, D;Shih, HY;Jiang, K;Hudson, WH;Brooks, SR;Sun, HW;Villarino, AV;Yao, C;Singleton, K;Akondy, RS;Kanno, Y;Sher, A;Casellas, R;Ahmed, R;O'Shea, JJ; | The Magnitude of IFN-γ Responses Is Fine-Tuned by DNA Architecture and the Non-coding Transcript of Ifng-as1 | Mol. Cell | For LCMV experiments RNA was isolated from sorted cells of LCMV-infected mice with the QIAGEN AllPrep Micro Kit. Libraries were prepared and sequenced by the HudsonAlpha Genomic Services Lab (paired-end, 150 bp reads). For all other experiments total | Full Paper | |
2019 | Kwiatkowski, J;Thompson, A;Rasko, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;Schmidt, M;Kletzel, M;Vichinsky, E;Deary, B;Chen, Y;Petrusich, A;Walters, M; | Long-Term Clinical Outcomes of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia in the Northstar (HGB-204) Study | Blood | Background Patients with transfusion-dependent β-thalassemia (TDT) may experience transfusional iron overload and end-organ damage. While potentially curative, allogeneic hematopoietic stem cell (HSC) transplantation is limited by tran | Full Paper | |
2019 | Furie, R;Werth, VP;Merola, JF;Stevenson, L;Reynolds, TL;Naik, H;Wang, W;Christmann, R;Gardet, A;Pellerin, A;Hamann, S;Auluck, P;Barbey, C;Gulati, P;Rabah, D;Franchimont, N; | Monoclonal antibody targeting BDCA2 ameliorates skin lesions in systemic lupus erythematosus | J. Clin. Invest. | 30645203 | ... un-exposed skin of HVs matched approximately by age and ethnicity to the BIIB059-treated cohort (mean age = 34; range, 28–37; White, 6/8; Asian, 1/8; Black or African American, 1/8; female, 8/8) by Folio Biosciences, then processed into paraffin | Full Paper |
2019 | Hudson, WH;Prokhnevska, N;Gensheimer, J;Akondy, R;McGuire, DJ;Ahmed, R;Kissick, HT; | Expression of novel long noncoding RNAs defines virus-specific effector and memory CD8+ T cells | Nat Commun | 30643116 | ... of Emory University. RNA Isolation and sequencing RNA was isolated from sorted cells of LCMV-infected mice with the Qiagen AllPrep Micro Kit. Library preparation and sequencing were performed by the HudsonAlpha Genomic Services Laboratory via th | Full Paper |
2019 | Ma, X;Shao, Y;Tian, L;Flasch, DA;Mulder, HL;Edmonson, MN;Liu, Y;Chen, X;Newman, S;Nakitandwe, J;Li, Y;Li, B;Shen, S;Wang, Z;Shurtleff, S;Robison, LL;Levy, S;Easton, J;Zhang, J; | Analysis of error profiles in deep next-generation sequencing data | Genome Biol. | 30867008 | Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA. Xiaotu.Ma@stjude.org.++Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.++Department of Computatio | Full Paper |
2019 | Zeng, Q;Liao, C;Terhune, J;Wang, L; | Impacts of florfenicol on the microbiota landscape and resistome as revealed by metagenomic analysis | Microbiome | 31818316 | Carlsbad, CA) according to the manufacturer's instructions. The library construction and sequencing were conducted at the HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). Genomic libraries were prepared with the | Full Paper |
2019 | Lee, CR;Yonk, AJ;Wiskerke, J;Paradiso, KG;Tepper, JM;Margolis, DJ; | Opposing Influence of Sensory and Motor Cortical Input on Striatal Circuitry and Choice Behavior | Curr. Biol. | 30982651 | Light was delivered to the cortical afferents and PV interneurons in the striatum through an optical fiber patchcord (Thorlabs; length 1 m; 0.50 nA; 200 mm diameter) connected to an optical cannula (described above) via a mating sleeve (Thorlabs). A | Full Paper |
2019 | Ramachandran, I;Lowther, DE;Dryer-Minnerly, R;Wang, R;Fayngerts, S;Nunez, D;Betts, G;Bath, N;Tipping, AJ;Melchiori, L;Navenot, JM;Glod, J;Mackall, CL;D'Angelo, SP;Araujo, DM;Chow, WA;Demetri, GD;Druta, M;Van Tine, BA;Grupp, SA;Abdul Razak, AR;Wilky, B;Iyengar, M;Trivedi, T;Winkle, EV;Chagin, K;Amado, R;Binder, GK;Basu, S; | Systemic and local immunity following adoptive transfer of NY-ESO-1 SPEAR T cells in synovial sarcoma | J Immunother Cancer | 31651363 | d Cross (Philadelphia, PA). NY-ESO-1 testing was performed via IHC at a Clinical Laboratory Improvement Amendments certified pathology laboratory at the National Cancer Institute (Bethesda, MD) or at QualTek Labs (Goleta, CA). Disease response was cl | Full Paper |
2019 | Spino, M;Kurz, SC;Chiriboga, L;Serrano, J;Zeck, B;Sen, N;Patel, S;Shen, G;Vasudevaraja, V;Tsirigos, A;Suryadevara, CM;Frenster, JD;Tateishi, K;Wakimoto, H;Jain, R;Riina, HA;Nicolaides, TP;Sulman, EP;Cahill, DP;Golfinos, JG;Isse, K;Saunders, LR;Zagzag, D;Placantonakis, DG;Snuderl, M;Chi, AS; | Cell Surface Notch Ligand DLL3 is a Therapeutic Target in Isocitrate Dehydrogenase-mutant Glioma | Clin. Cancer Res. | 30397180 | For the discovery set, 20 nontumor brain tissue samples and 63 glioma tumor samples were obtained from Cooperative Human Tumor Network (CHTN), Conversant Bio, and RS Diagnostics. This set of tumors included the following diagnoses: glioblastoma, WHO | Full Paper |
2019 | Habra, MA;Stephen, B;Campbell, M;Hess, K;Tapia, C;Xu, M;Rodon Ahnert, J;Jimenez, C;Lee, JE;Perrier, ND;Boraddus, RR;Pant, S;Subbiah, V;Hong, DS;Zarifa, A;Fu, S;Karp, DD;Meric-Bernstam, F;Naing, A; | Phase II clinical trial of pembrolizumab efficacy and safety in advanced adrenocortical carcinoma | J Immunother Cancer | 31533818 | PD-L1 staining was performed by Qualtek using Merck 22C3 antibody for PD-L1 and scored by a board-certified pathologist. Based on the percentage and intensity of membrane staining, H-score, ranging from 0 to 300, was assigned to tumor samples ;i | Full Paper |
2019 | Lv, JW;Li, JY;Luo, LN;Wang, ZX;Chen, YP; | Comparative safety and efficacy of anti-PD-1 monotherapy, chemotherapy alone, and their combination therapy in advanced nasopharyngeal carcinoma: findings from recent advances in landmark trials | J Immunother Cancer | 31238988 | D-L1 expression was assessed baseline on an archived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy sample using a laboratory-developed prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA). PD-L1 | Full Paper |
2019 | Clouthier, DL;Lien, SC;Yang, SYC;Nguyen, LT;Manem, VSK;Gray, D;Ryczko, M;Razak, ARA;Lewin, J;Lheureux, S;Colombo, I;Bedard, PL;Cescon, D;Spreafico, A;Butler, MO;Hansen, AR;Jang, RW;Ghai, S;Weinreb, I;Sotov, V;Gadalla, R;Noamani, B;Guo, M;Elston, S;Giesler, A;Hakgor, S;Jiang, H;McGaha, T;Brooks, DG;Haibe-Kains, B;Pugh, TJ;Ohashi, PS;Siu, LL; | An interim report on the investigator-initiated phase 2 study of pembrolizumab immunological response evaluation (INSPIRE) | J Immunother Cancer | 30867072 | IHC. For screening biopsies only, the FFPE blocks were used for PD-L1 IHC (clone 22C3) on 4-5 μm sections mounted on positively charged ProbeOn slides (QualTek, Goleta, CA). QualTek provided a modified proportion score ;from the same site as the | Full Paper |
2019 | Rowley, MJ;Lyu, X;Rana, V;Ando-Kuri, M;Karns, R;Bosco, G;Corces, VG; | Condensin II Counteracts Cohesin and RNA Polymerase II in the Establishment of 3D Chromatin Organization | Cell Rep | 30865881 | We would like to thank the HudsonAlpha Institute for Biotechnology Genomic Services Lab for their help with Illumina sequencing. This work was supported by NIH Pathway to Independence Award K99/R00 GM127671 (M.J.R.) and U.S. Public Health Service Awa | Full Paper |
2019 | Wu, T;Dejanovic, B;Gandham, VD;Gogineni, A;Edmonds, R;Schauer, S;Srinivasan, K;Huntley, MA;Wang, Y;Wang, TM;Hedehus, M;Barck, KH;Stark, M;Ngu, H;Foreman, O;Meilandt, WJ;Elstrott, J;Chang, MC;Hansen, DV;Carano, RAD;Sheng, M;Hanson, JE; | Complement C3 Is Activated in Human AD Brain and Is Required for Neurodegeneration in Mouse Models of Amyloidosis and Tauopathy | Cell Rep | 31433986 | Human CSF cohort 1 Folio Biosciences n/a Human CSF cohort 2 PrecisionMed n/a Frozen human brain tissue Folio Biosciences n/ | Full Paper |
2019 | Angelova, E;Audette, C;Kovtun, Y;Daver, N;Wang, SA;Pierce, S;Konoplev, SN;Khogeer, H;Jorgensen, JL;Konopleva, M;Zweidler-McKay, PA;Medeiros, LJ;Kantarjian, HM;Jabbour, EJ;Khoury, JD; | CD123 expression patterns and selective targeting with a CD123-targeted antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemia | Haematologica | 30361418 | ... In vitro evaluation of primary B-cell acute lymphoblastic leukemia samples Bone marrow mononuclear cells from 11 newly diagnosed and 10 relapsed/refractory B-ALL patients were obtained from MDACC or ConversantBio. The number of CD123 antibody-bi | Full Paper |
2019 | Bao, L;Tian, C;Liu, S;Zhang, Y;Elaswad, A;Yuan, Z;Khalil, K;Sun, F;Yang, Y;Zhou, T;Li, N;Tan, S;Zeng, Q;Liu, Y;Li, Y;Li, Y;Gao, D;Dunham, R;Davis, K;Waldbieser, G;Liu, Z; | The Y chromosome sequence of the channel catfish suggests novel sex determination mechanisms in teleost fish | BMC Biol. | 30683095 | ;... Bioanalyzer. Equal amounts of RNA from each 5-day period were pooled from 10 to 14 dpf and 15 to 19 dpf for male and female, respectively. These four pooled samples were outsourced for RNA-Seq at the HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2019 | Rieker, C;Migliavacca, E;Vaucher, A;Mayer, FC;Baud, G;Marquis, J;Charpagne, A;Hegde, N;Guignard, L;McLachlan, M;Pooler, AM; | Apolipoprotein E4 Expression Causes Gain of Toxic Function in Isogenic Human Induced Pluripotent Stem Cell-Derived Endothelial Cells | Arterioscler. Thromb. Vasc. Biol. | 31315437 | Immunohistochemistry Analysis of Postmortem Human Brain Tissue Human hippocampal brain tissue from AD (Braak stage V-VI; both male and female) individuals was purchased from Folio Biosciences, genotyped for ApoE, and processed for cryo- sectioning | Full Paper |
2019 | Smith, A;Schiller, G;Vercellotti, G;Kwiatkowski, J;Krishnamurti, L;Esrick, E;Williams, D;Miller, W;Woolfson, A;Walters, M; | Preliminary Results of a Phase 1/2 Clinical Study of Zinc Finger Nuclease-Mediated Editing of BCL11A in Autologous Hematopoietic Stem Cells for Transfusion-Dependent Beta Thalassemia | Blood | Introduction: Persistently high fetal hemoglobin (HbF) expression can ameliorate severe transfusion-dependent beta thalassemia (TDT). BCL11A, a master regulator of the fetal-to-adult hemoglobin switch, is a rational gene-editing target in beta globin | Full Paper | |
2019 | Kang, J;Yeom, G;Jang, H;Oh, J;Park, CJ;Kim, MG; | Development of Replication Protein A-Conjugated Gold Nanoparticles for Highly Sensitive Detection of Disease Biomarkers | Anal. Chem. | 31269392 | Patient samples for NP and cTnI were obtained from Discovery Life Sciences, Inc. The study protocol was thoroughly explained to the patients, and signed written informed consent forms were obtained in accordance with the approved guidelines and relev | Full Paper |
2019 | Planes-Laine, G;Rochigneux, P;Bertucci, F;Chrétien, AS;Viens, P;Sabatier, R;Gonçalves, A; | PD-1/PD-L1 Targeting in Breast Cancer: The First Clinical Evidences Are Emerging. A Literature Review | Cancers (Basel) | 31336685 | ts included locally advanced or metastatic disease, after a failure of or inability to receive standard therapy, ECOG PS 0 or 1, ≥ 1 measurable lesion and PD-L1 positivity, using a prototype assay (QualTek Molecular Laboratories, Goleta, CA, USA) and | Full Paper |
2019 | Bernard-Gauthier, V;Mossine, AV;Knight, A;Patnaik, D;Zhao, WN;Cheng, C;Krishnan, HS;Xuan, LL;Chindavong, PS;Reis, SA;Chen, JM;Shao, X;Stauff, J;Arteaga, J;Sherman, P;Salem, N;Bonsall, D;Amaral, B;Varlow, C;Wells, L;Martarello, L;Patel, S;Liang, SH;Kurumbail, RG;Haggarty, SJ;Scott, PJH;Vasdev, N; | Structural Basis for Achieving GSK-3β Inhibition with High Potency, Selectivity, and Brain Exposure for Positron Emission Tomography Imaging and Drug Discovery | J. Med. Chem. | 31535859 | For autoradiography experiments, fresh-frozen postmortem human brain tissue blocks were acquired from Folio-Conversant and the University of California at San Francisco NDBB. In vitro autoradiography studies were performed as described in the Support | Full Paper |
2019 | McCoy, CR;Glover, ME;Flynn, LT;Simmons, RK;Cohen, JL;Ptacek, T;Lefkowitz, EJ;Jackson, NL;Akil, H;Wu, X;Clinton, SM; | Altered DNA Methylation in the Developing Brains of Rats Genetically Prone to High versus Low Anxiety | J. Neurosci. | 30683683 | Starting material for the enrichment protocol was 1g of sonicated DNA, and captured fragments were eluted using 2500 mM NaCl per the manufacturer’s instructions and as described previously (Brinkman et al., 2010). Captured material was purified using | Full Paper |
2019 | Chiu, H;Trisal, P;Bjorklund, C;Carrancio, S;Toraño, EG;Guarinos, C;Papazoglou, D;Hagner, PR;Beldi-Ferchiou, A;Tarte, K;Delfau-Larue, MH;Morschhauser, F;Ramsay, AG;Gandhi, AK; | Combination lenalidomide-rituximab immunotherapy activates anti-tumour immunity and induces tumour cell death by complementary mechanisms of action in follicular lymphoma | Br. J. Haematol. | 30767211 | Viably‐frozen PBMC from naive and relapsed/refractory FL and MZL patients were purchased from ConversantBio (Huntsville, AL, USA), ProteoGenex, (Inglewood, CA, USA) and BioreclammationIVT (Hicksville, NY, USA). Cell lines and cell culture | Full Paper |
2019 | Liang, X;Zhuang, J;Löffler, FE;Zhang, Y;DeBruyn, JM;Wilhelm, SW;Schaeffer, SM;Radosevich, M; | Viral and bacterial community responses to stimulated Fe(III)-bioreduction during simulated subsurface bioremediation | Environ. Microbiol. | 30773777 | Whole soil DNA was obtained by extracting both soil aggregates and associated pore water (Cápiro et al., 2014) using PowerLyser PowerSoil DNA isolation kit (MoBio Laboratories Inv. Carlsbad, CA) following the manufacturer’s instructions. The extracte | Full Paper |
2019 | Nowinski, B;Motard-Côté, J;Landa, M;Preston, CM;Scholin, CA;Birch, JM;Kiene, RP;Moran, MA; | Microdiversity and temporal dynamics of marine bacterial dimethylsulfoniopropionate genes | Environ. Microbiol. | 30761723 | This article is protected by copyright. All rights reserved. Page 23. HiSeq Rapid Run at HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA). Metagenomic data are deposited in the NCBI SRA under project number PRJNA505827. Single Amplified | Full Paper |
2019 | Xu, H;Muise, ES;Javaid, S;Chen, L;Cristescu, R;Mansueto, MS;Follmer, N;Cho, J;Kerr, K;Altura, R;Machacek, M;Nicholson, B;Addona, G;Kariv, I;Chen, H; | Identification of predictive genetic signatures of Cytarabine responsiveness using a 3D acute myeloid leukaemia model | J. Cell. Mol. Med. | 31449347 | 2 METHODS. 2.1 Cells and reagents. Normal and AML patient BMMCs were purchased from Folio Conversant and ProteoGenex. Recombinant human fibronectin was purchased from Millipore. Rat tail collagen type 1 and matrigel were purchased from Corning Inc | Full Paper |
2019 | Kim, YJ;Keam, B;Ock, CY;Song, S;Kim, M;Kim, SH;Kim, KH;Kim, JS;Kim, TM;Kim, DW;Lee, JS;Heo, DS; | A phase II study of pembrolizumab and paclitaxel in patients with relapsed or refractory small-cell lung cancer | Lung Cancer | 31494530 | Informed consent for specimens be obtained during screening for protocol enrollment from all subjects or legally acceptable representative, at a trial visit by the investigator or designate. Pretreatment blood sampling and tumor biopsies will be done | Full Paper |
2019 | Yang, X;Saito, Y;Rao, A;Kim, HJ;Singh, P;Scott, E;Larson, M;Pan, W;Desai, M;Hubbell, E; | Alignment-free filtering for cfNA fusion fragments | Bioinformatics | 31510681 | We have obtained 40 ml of whole blood collected in Streck tubes, separated into plasma and extracted cfRNA samples from two Stage IV Prostate cancer patients and three healthy controls from Conversant Bio and generated cfRNA sequencing data with UMIs | Full Paper |
2019 | Qi, Z;Wang, L;Desai, K;Cogswell, J;Stern, M;Lawson, B;Kerkar, SP;Vitazka, P; | Reliable Gene Expression Profiling from Small and Hematoxylin and Eosin-Stained Clinical Formalin-Fixed, Paraffin-Embedded Specimens Using the HTG EdgeSeq Platform | J Mol Diagn | 31255795 | Rockville, MD). Small-cell lung cancer (SCLC) samples (n = 4) were purchased from Conversant Biologics, Inc. (Huntsville, AL) or Proteogenex (Inglewood, CA). FFPE muscle tissue was obtained from Proteogenex. RNA samples | Full Paper |
2019 | Dietz, L;Dömel, JS;Leese, F;Mahon, AR;Mayer, C; | Phylogenomics of the longitarsal Colossendeidae: The evolutionary history of an Antarctic sea spider radiation | Mol. Phylogenet. Evol. | 31002869 | Sea spiders (Pycnogonida) constitute a group of marine benthic arthropods that has a particularly high species diversity in the Southern Ocean. The "longitarsal" group of the sea spider family Colossendeidae is especially abundant in this region. How | Full Paper |
2019 | Varga, A;Piha-Paul, S;Ott, PA;Mehnert, JM;Berton-Rigaud, D;Morosky, A;Yang, P;Ruman, J;Matei, D; | Pembrolizumab in patients with programmed death ligand 1-positive advanced ovarian cancer: Analysis of KEYNOTE-028 | Gynecol. Oncol. | 30522700 | disease based on Response Evaluation Criteria in Solid Tumors version 1.1 (RECIST v1.1), PD-L1 positivity, determined immunohistochemically and defined as membranous staining on ≥1% modified proportion score or interface pattern (QualTek Molecular La | Full Paper |
2019 | Lee, MJ;Miller, Z;Park, JE;Bhattarai, D;Lee, W;Kim, KB; | H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth | Sci Rep | 30858500 | ... on-linear regression based on n = 3 replicates per compound per concentration. (d) Heat map showing proteasome catalytic subunit activity profiles of 6 PI-naïve patient MM samples purchased from Conversant Biologics and AllCells (left). The numbe | Full Paper |
2019 | Liang, X;Radosevich, M;Löffler, F;Schaeffer, SM;Zhuang, J; | Impact of microbial iron oxide reduction on the transport of diffusible tracers and non-diffusible nanoparticles in soils | Chemosphere | 30597359 | Inc. Carlsbad, California, USA). The DNA samples were quantified using PicoGreen Assay Kit (Carlsbad, California, USA) and sent out for sequencing at HudsonAlpha Genomic Services Lab (Huntsville, Alabama, USA). The | Full Paper |
2019 | Nicolini, F;Bocchini, M;Bronte, G;Delmonte, A;Guidoboni, M;Crinò, L;Mazza, M; | Malignant Pleural Mesothelioma: State-of-the-Art on Current Therapies and Promises for the Future | Front Oncol | 32039010 | With a centrally-assessed 22C3 antibody-based immunohistochemistry assay (Qualtek laboratory), PDL1 expression was available in 62 patients: 45% were found negative ( | Full Paper |
2019 | Gani, AW;Wei, W;Shi, RZ;Ng, E;Nguyen, M;Chua, MS;So, S;Wang, SX; | An Automated, Quantitative, and Multiplexed Assay Suitable for Point-of-Care Hepatitis B Virus Diagnostics | Sci Rep | 31666635 | HBV DNA levels). As controls, we recruited 5 healthy volunteers who had no prior history of HBV and who were vaccinated against HBV. Serum samples from healthy Asian donors were also purchased from Conversant Biologics (Huntsville, AL). Supplementa | Full Paper |
2019 | Li, Y;Kocot, KM;Tassia, MG;Cannon, JT;Bernt, M;Halanych, KM; | Mitogenomics Reveals a Novel Genetic Code in Hemichordata | Genome Biol Evol | 30476024 | Variant codes in mitochondrial genomes within deuterostomes and photos from representatives of hemichordates. (A) Phylogenetic relationships according to the current understanding of deuterostome phylogeny based on multiple studies, red bars show cod | Full Paper |
2019 | Juttukonda, LJ;Green, ER;Lonergan, ZR;Heffern, MC;Chang, CJ;Skaar, EP; | Acinetobacter baumannii OxyR Regulates the Transcriptional Response to Hydrogen Peroxide | Infect. Immun. | 30297527 | RNA-seq library construction and sequencing were performed by HudsonAlpha (Huntsville, AL). The concentration and integrity of the total RNA were estimated with a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA) and an Agilent 2100 bioanalyzer (Appli | Full Paper |
2019 | Patel, S;Knight, A;Krause, S;Teceno, T;Tresse, C;Li, S;Cai, Z;Gouasmat, A;Carroll, VM;Barret, O;Gottmukkala, V;Zhang, W;Xiang, X;Morley, T;Huang, Y;Passchier, J; | Preclinical In Vitro and In Vivo Characterization of Synaptic Vesicle 2A-Targeting Compounds Amenable to F-18 Labeling as Potential PET Radioligands for Imaging of Synapse Integrity | Mol Imaging Biol | 31728839 | The same procedure was used for human post-mortem tissue (Folio Biosciences, Miami Brain Bank, and UCSF Memory and Aging Center, USA). In Vitro Homogenate Binding. All homogenate studies were carried out in 96-well format as previously described [14] | Full Paper |
2019 | Gao, L;Yuan, Z;Zhou, T;Yang, Y;Gao, D;Dunham, R;Liu, Z; | FOXO genes in channel catfish and their response after bacterial infection | Dev. Comp. Immunol. | 30905685 | ; Plus Kit (Qiagen). RNA-seq library was prepared and sequenced by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) using an Illumina HiSeq 2000 instrument with 100 bp paired end (PE) reads. The trimmed high | Full Paper |
2019 | Havird, JC;Meyer, E;Fujita, Y;Vaught, RC;Henry, RP;Santos, SR; | Disparate responses to salinity across species and organizational levels in anchialine shrimps | J. Exp. Biol. | 31727759 | Assembly of reference transcriptomes was previously described for H. rubra (Havird and Santos, 2016a) and the four species from Japan (Havird et al., 2014b). Briefly, total RNA was extracted from whole animals 1 month after preservation in RNAlater v | Full Paper |
2019 | Rey, EG;Finkelstein, JL;Erickson, D; | Fluorescence lateral flow competitive protein binding assay for the assessment of serum folate concentrations | PLoS ONE | 31166978 | Serum sample preparation. Calibration curves made with human serum spiked with FA and 5-MTHF were performed as part of a previous work[26]. Human serum samples of varying folate concentration were purchased from Discovery Life Sciences Inc. (Los Osos | Full Paper |
2019 | Mehnert, JM;Varga, A;Brose, MS;Aggarwal, RR;Lin, CC;Prawira, A;de Braud, F;Tamura, K;Doi, T;Piha-Paul, SA;Gilbert, J;Saraf, S;Thanigaimani, P;Cheng, JD;Keam, B; | Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with advanced, PD-L1-positive papillary or follicular thyroid cancer | BMC Cancer | 30832606 | biopsy sample. PD-L1 expression was assessed using a prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, California) [22] and the 22C3 antibody (Merck & Co., Inc., Kenilworth, New Jersey). PD ;screening period using ei | Full Paper |
2019 | Huang, RSP;Holmes, BF;Powell, C;Marati, RV;Tyree, D;Admire, B;Streator, A;Newell, AEH;Perez, J;Dalvi, D;ElGabry, EA; | Delta-like Protein 3 Prevalence in Small Cell Lung Cancer and DLL3 (SP347) Assay Characteristics | Arch. Pathol. Lab. Med. | 30958693 | formalin), paraffin-embedded SCLC whole-section samples was procured from an internal tissue bank at Ventana Medical Systems, Inc, and the following commercial tissue banks: Asterand Bioscience; Avaden BioSciences; Boca Biolistics; Conversant Bio; GL | Full Paper |
2019 | Tsypin, M;Asmellash, S;Meyer, K;Touchet, B;Roder, H; | Extending the information content of the MALDI analysis of biological fluids via multi-million shot analysis | PLoS ONE | 31815946 | 100 serum samples were purchased from the commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrics (Fort Worth, TX). Samples were collected under ethics-approved protocols according to the requirements of Conversant Bio and Oncology M | Full Paper |
2019 | Li, S;Song, C;Yang, S;Yu, W;Zhang, W;Zhang, G;Xi, Z;Lu, E; | Supercritical CO2 foamed composite scaffolds incorporating bioactive lipids promote vascularized bone regeneration via Hif-1α upregulation and enhanced type H vessel formation | Acta biomaterialia | 31154054 | Upon reaching 80-90% confluency, the rBMSCs were passaged with passages 2-4 being used for further experiments. HUVECs (AllCells, Shanghai, China) were cultured in endothelial cell medium (ECM, ScienCell, USA) under the same conditions and used for i | Full Paper |
2019 | Desai, SP;Coston, A;Berlin, A; | Micro-Electrical Impedance Spectroscopy and Identification of Patient-Derived, Dissociated Tumor Cells | IEEE Trans Nanobioscience | 31180894 | Stored waveforms are further analyzed using statistical data analysis techniques. C. Dissociated Tumor Cells and Cell Culture Dissociated tumor cells (DTCs) were acquired directly from Conversant Bio (Huntsville, AL) for a range of indications and ca | Full Paper |
2019 | Lee, JS;Rosoff, D;Luo, A;Longley, M;Phillips, M;Charlet, K;Muench, C;Jung, J;Lohoff, FW; | PCSK9 is Increased in Cerebrospinal Fluid of Individuals With Alcohol Use Disorder | Alcohol. Clin. Exp. Res. | 30933362 | the NIH Institutional Review Board (IRB). All participants provided written informed consent before participation. CSF of deidentified controls was purchased from Discovery Life Sciences, Inc. (Research Biospecimen Sample Bank, Los Osos, CA, USA) | Full Paper |
2019 | Burbelo, PD;Chaturvedi, A;Notkins, AL;Gunti, S; | Luciferase-Based Detection of Antibodies for the Diagnosis of HPV-Associated Head and Neck Squamous Cell Carcinoma | Diagnostics (Basel) | 31390810 | The serum samples from colon cancer (CC), systemic lupus erythematosus (SLE), and HNSCC patients used in this report were obtained from Conversant Bio (601 Genome Way Suite 1200, Huntsville, Alabama 35806). Subjects | Full Paper |
2019 | Kao, JH;Lin, CY;Chuang, WL;Cheng, YY;Hu, JY;Liang, WK;Friebe, P;Palmer, S;Huang, CS; | Clinical evaluation of IntelliPlexTM HCV genotyping kit for hepatitis C virus genotyping | Diagn. Microbiol. Infect. Dis. | 30975461 | as certified by the commercial supplier in the accompanying Certificate of Analysis (CoA)), including genotypes 3, 4 and 5 were spiked into the sample pool (samples were obtained from SeraCare Life Sciences Inc., [Cambridge, MA United States]; Discov | Full Paper |
2019 | Lagatie, O;Verheyen, A;Nijs, E;Batsa Debrah, L;Debrah, YA;Stuyver, LJ; | Performance evaluation of 3 serodiagnostic peptide epitopes and the derived multi-epitope peptide OvNMP-48 for detection of Onchocerca volvulus infection | Parasitol. Res. | 31089811 | and informed consent, which was signed by all subjects. For samples obtained from Tissue Solutions Ltd. (Glasgow, Scotland), Discovery Life Sciences, Inc. (Los Osos, USA), Bioreclamation IVT (Baltimore, USA), Mayo Clinic | Full Paper |
2019 | Montazeri, M;Marbut, C;Huitink, D; | Interconnect Fatigue Failure Parameter Isolation for Power Device Reliability Prediction in Alternative Accelerated Mechanical Cycling Test | Journal of Electronic Packaging | IEEE Forth Workshop on Wide Bandgap Power Devices and Applications ( WiPDA), Fayetteville, AR, Nov. 7-9, pp. 194– 199. 19. Qualitek, 2014, “Technical Data Sheet: Sn42/Bi58 Solder Wire,” Qualtek Electronics Corporation, Addison, IL, Accessed Ma | Full Paper | |
2019 | Mohan, S;Lawton, R;Palmer, C;Rojas, AC; | Competitive ELISA method for novel estrogen-negative breast cancer biomarker quantitation | J. Immunol. Methods | 31533022 | system) (Rakha et al., 2008), and categorized based on estrogen-hormone-receptor orientation (viz., ER − versus ER + , n = 50), were obtained from cooperative human tissue network (Philadelphia, PA), Tissue-for-Research (Atlanta, GA), and Conversant | Full Paper |
2019 | Nicol, AF;de Andrade, CV;Gomes, SC;Brusadelli, MG;Lodin, HM;Wells, SI;Nuovo, GJ; | The distribution of novel biomarkers in carcinoma-in-situ, microinvasive, and squamous cell carcinoma of the uterine cervix | Ann Diagn Pathol | 30579259 | National Institute of Infectious Diseases Evandro Chagas - INI-Fiocruz, Rio de Janeiro, Brazil.++National Institute of Health of Women, Children, and Adolescents, Fernandes Figueira - IFF-FIOCRUZ, Rio de Janeiro, Brazil.++National Institute of Health | Full Paper |
2019 | Mimitou, EP;Cheng, A;Montalbano, A;Hao, S;Stoeckius, M;Legut, M;Roush, T;Herrera, A;Papalexi, E;Ouyang, Z;Satija, R;Sanjana, NE;Koralov, SB;Smibert, P; | Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells | Nature methods | 31011186 | FBS 10% DMSO and aliquots of 1-1.5 million cells were thawed on the day of the experiment. PBMCs were obtained cryopreserved from AllCells (USA) and used immediately after thawing. NIH-3T3 and HEK293FT cells expressing non-targeting sgRNAs were maint | Full Paper |
2019 | Symeonides, S; | Choice of Law in the American Courts in 2018: Thirty-Second Annual Survey | The American Journal of Comparative Law | In re EP Floors Corp., No. 14-18-00610-CV, 2018 WL 4354688 (Tex. App. Sept. 13, 2018); In re Rosewood Private Invs., Inc., No. 05-18-00166-CV, 2018 WL 4403749 (Tex. App. Sept. 17, 2018); Bundy v. Adesa Houston D/B/A Adesa, Inc., No. 01-17-00863-CV, 2 | Full Paper | |
2019 | Yant, SR;Mulato, A;Hansen, D;Tse, WC;Niedziela-Majka, A;Zhang, JR;Stepan, GJ;Jin, D;Wong, MH;Perreira, JM;Singer, E;Papalia, GA;Hu, EY;Zheng, J;Lu, B;Schroeder, SD;Chou, K;Ahmadyar, S;Liclican, A;Yu, H;Novikov, N;Paoli, E;Gonik, D;Ram, RR;Hung, M;McDougall, WM;Brass, AL;Sundquist, WI;Cihlar, T;Link, JO; | A highly potent long-acting small-molecule HIV-1 capsid inhibitor with efficacy in a humanized mouse model | Nature medicine | 31501601 | Human PBMCs were collected from consenting healthy volunteers under informed consent; their use was approved by an institutional review board at AllCells. Human PBMCs, CD4 + T Save | Full Paper |
2019 | Qu, L;Yi, Z;Zhu, S;Wang, C;Cao, Z;Zhou, Z;Yuan, P;Yu, Y;Tian, F;Liu, Z;Bao, Y;Zhao, Y;Wei, W; | Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs | Nature biotechnology | 31308540 | 10% FBS and IL2 (1,000 U ml-1) and stimulated with CD3/CD28 DynaBeads (ThermoFisher, 11131D) for 2 d. Leukapheresis products from healthy donors were acquired from AllCells LLC China. All healthy donors provided informed consent | Full Paper |
2019 | Granja, JM;Klemm, S;McGinnis, LM;Kathiria, AS;Mezger, A;Corces, MR;Parks, B;Gars, E;Liedtke, M;Zheng, GXY;Chang, HY;Majeti, R;Greenleaf, WJ; | Single-cell multiomic analysis identifies regulatory programs in mixed-phenotype acute leukemia | Nature biotechnology | 31792411 | Description of healthy donors. PBMCs, BMMCs and CD34+ bone marrow cells were obtained from healthy donors with informed consent and compliance with relevant ethical regulations (AllCells). Individual information for each donor is provided in Suppleme | Full Paper |
2019 | Satpathy, AT;Granja, JM;Yost, KE;Qi, Y;Meschi, F;McDermott, GP;Olsen, BN;Mumbach, MR;Pierce, SE;Corces, MR;Shah, P;Bell, JC;Jhutty, D;Nemec, CM;Wang, J;Wang, L;Yin, Y;Giresi, PG;Chang, ALS;Zheng, GXY;Greenleaf, WJ;Chang, HY; | Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion | Nature biotechnology | 31375813 | obtained from AllCells or the AllCells and isolated per manufacturer recommendations (as outlined in Supplementary Table 4). FACS-isolated populations were obtained from AllCells Save | Full Paper |
2019 | Miller, JC;Patil, DP;Xia, DF;Paine, CB;Fauser, F;Richards, HW;Shivak, DA;Bendaña, YR;Hinkley, SJ;Scarlott, NA;Lam, SC;Reik, A;Zhou, Y;Paschon, DE;Li, P;Wangzor, T;Lee, G;Zhang, L;Rebar, EJ; | Enhancing gene editing specificity by attenuating DNA cleavage kinetics | Nature biotechnology | 31359006 | CD34 + stem and progenitor cells were purified from mobilized peripheral blood leukapheresis products obtained from AllCells. Platelet depletion was performed, CD34 + cells were Save | Full Paper |
2019 | Kinney, MA;Vo, LT;Frame, JM;Barragan, J;Conway, AJ;Li, S;Wong, KK;Collins, JJ;Cahan, P;North, TE;Lauffenburger, DA;Daley, GQ; | A systems biology pipeline identifies regulatory networks for stem cell engineering | Nature biotechnology | 31267104 | Human CD34 + progenitors derived from mobilized peripheral blood (AllCells) were expanded for 4 d in StemSpan SFEM (StemCell Technologies) with the addition of interleukin (IL)-3 ( Save | Full Paper |
2019 | Lareau, CA;Duarte, FM;Chew, JG;Kartha, VK;Burkett, ZD;Kohlway, AS;Pokholok, D;Aryee, MJ;Steemers, FJ;Lebofsky, R;Buenrostro, JD; | Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibility | Nature biotechnology | 31235917 | CD34+ stem/progenitor cells, peripheral blood mononuclear cells (PBMC), and isolated peripheral blood CD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from Allcells ( Save;BMMCs, isolated bone marrow CD34 + stem/progenitor cells, PBMCs, and i | Full Paper |
2019 | Setty, M;Kiseliovas, V;Levine, J;Gayoso, A;Mazutis, L;Pe'er, D; | Characterization of cell fate probabilities in single-cell data with Palantir | Nature biotechnology | 30899105 | incorporate lineage information to model cell fate decisions. scRNA-seq of CD34+ human bone marrow cells Cryopreserved bone marrow stem/progenitor CD34+ cells from healthy donors were purchased from AllCells, LLC. (Cat. No. ABM022F) and stored in va | Full Paper |
2019 | Shah, NJ;Mao, AS;Shih, TY;Kerr, MD;Sharda, A;Raimondo, TM;Weaver, JC;Vrbanac, VD;Deruaz, M;Tager, AM;Mooney, DJ;Scadden, DT; | An injectable bone marrow-like scaffold enhances T cell immunity after hematopoietic stem cell transplantation | Nature biotechnology | 30742125 | t-specific antibodies. We isolated hematopoietic cells (Lin−Sca-1hic-kithi) using a FacsAria cell sorter (BD). Sorted cells were ≥ 95% pure. Human cord-blood derived CD34+ cells were purchased (Allcells) and expanded for seven days using expansion su | Full Paper |
2019 | Maun, HR;Jackman, JK;Choy, DF;Loyet, KM;Staton, TL;Jia, G;Dressen, A;Hackney, JA;Bremer, M;Walters, BT;Vij, R;Chen, X;Trivedi, NN;Morando, A;Lipari, MT;Franke, Y;Wu, X;Zhang, J;Liu, J;Wu, P;Chang, D;Orozco, LD;Christensen, E;Wong, M;Corpuz, R;Hang, JQ;Lutman, J;Sukumaran, S;Wu, Y;Ubhayakar, S;Liang, X;Schwartz, LB;Babina, M;Woodruff, PG;Fahy, JV;Ahuja, R;Caughey, GH;Kusi, A;Dennis, MS;Eigenbrot, C;Kirchhofer, D;Austin, CD;Wu, LC;Koerber, JT;Lee, WP;Yaspan, BL;Alatsis, KR;Arron, JR;Lazarus, RA;Yi, T; | An Allosteric Anti-tryptase Antibody for the Treatment of Mast Cell-Mediated Severe Asthma | Cell | 31585081 | Enriched CD34+ human cord blood stem cells were obtained from AllCells with more than 80% purity validated by flow cytometry analysis. CHO DKO cell line (Macaraeg et al., 2013) was a derivative of the CHO-K1 cell line (ATCC number CCL-61) was grown a | Full Paper |
2019 | Stuart, T;Butler, A;Hoffman, P;Hafemeister, C;Papalexi, E;Mauck, WM;Hao, Y;Stoeckius, M;Smibert, P;Satija, R; | Comprehensive Integration of Single-Cell Data | Cell | 31178118 | Hashtag 9 Cell Hashing antibody BioLegend Cat# 394617 Hashtag 10 Cell Hashing antibody BioLegend Cat# 394619 Biological Samples Human bone marrow cells AllCells Cat# ABM007F, lot 3008803 Critical Commercial Assays 3′ scRNA-seq kit 10x Genomics V2 che | Full Paper |
2019 | Abelin, JG;Harjanto, D;Malloy, M;Suri, P;Colson, T;Goulding, SP;Creech, AL;Serrano, LR;Nasir, G;Nasrullah, Y;McGann, CD;Velez, D;Ting, YS;Poran, A;Rothenberg, DA;Chhangawala, S;Rubinsteyn, A;Hammerbacher, J;Gaynor, RB;Fritsch, EF;Greshock, J;Oslund, RC;Barthelme, D;Addona, TA;Arieta, CM;Rooney, MS; | Defining HLA-II Ligand Processing and Binding Rules with Mass Spectrometry Enhances Cancer Epitope Prediction | Immunity | 31495665 | Human Healthy Donor Leukapheresis Material AllCells NA Human Healthy Donor Leukapheresis Material PPA Research NA Human Healthy Donor Leukapheresis Material StemExpress NA Human Healthy Donor Leukapheresis Material StemCell Technologies NA | Full Paper |
2019 | Lindner, JM;Cornacchione, V;Sathe, A;Be, C;Srinivas, H;Riquet, E;Leber, XC;Hein, A;Wrobel, MB;Scharenberg, M;Pietzonka, T;Wiesmann, C;Abend, J;Traggiai, E; | Human Memory B Cells Harbor Diverse Cross-Neutralizing Antibodies against BK and JC Polyomaviruses | Immunity | 30824324 | Purified peripheral blood mononuclear cells from leukapharesis AllCells, Inc. N/A leukapharesis and matched serum samples were obtained from AllCells LLC (Alameda, CA, USA). Save | Full Paper |
2019 | Li, R;Wang, Y;Zhang, X;Feng, M;Ma, J;Li, J;Yang, X;Fang, F;Xia, Q;Zhang, Z;Shang, M;Jiang, S; | Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasis | Molecular cancer | 30704479 | HUVECs were purchased from ATCC and cultivated in endothelial cell complete medium containing endothelial cell growth supplement (Allcells, USA). For hypoxic culture, HCC cells Save | Full Paper |
2019 | Jiang, Q;Isquith, J;Zipeto, MA;Diep, RH;Pham, J;Delos Santos, N;Reynoso, E;Chau, J;Leu, H;Lazzari, E;Melese, E;Ma, W;Fang, R;Minden, M;Morris, S;Ren, B;Pineda, G;Holm, F;Jamieson, C; | Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation | Cancer cell | 30612940 | Institutional Review Board approved protocol (#131550) that meets the requirements as stated in 45 CFR 46.404 and 21 CFR 50.51. De-identified (IRB exempt) human cord blood and normal aged-match samples were purchased as purified CD34+ cells from AllC | Full Paper |
2019 | Adelman, ER;Huang, HT;Roisman, A;Olsson, A;Colaprico, A;Qin, T;Lindsley, RC;Bejar, R;Salomonis, N;Grimes, HL;Figueroa, ME; | Aging Human Hematopoietic Stem Cells Manifest Profound Epigenetic Reprogramming of Enhancers That May Predispose to Leukemia | Cancer discovery | 31085557 | AML, which is more frequent in the elderly, is characterized by epigenetic deregulation. We demonstrate that epigenetic reprogramming of human HSCs occurs with age, affecting Save | Full Paper |
2019 | Gong, X;Du, J;Parsons, SH;Merzoug, FF;Webster, Y;Iversen, PW;Chio, LC;Van Horn, RD;Lin, X;Blosser, W;Han, B;Jin, S;Yao, S;Bian, H;Ficklin, C;Fan, L;Kapoor, A;Antonysamy, S;Mc Nulty, AM;Froning, K;Manglicmot, D;Pustilnik, A;Weichert, K;Wasserman, SR;Dowless, M;Marugán, C;Baquero, C;Lallena, MJ;Eastman, SW;Hui, YH;Dieter, MZ;Doman, T;Chu, S;Qian, HR;Ye, XS;Barda, DA;Plowman, GD;Reinhard, C;Campbell, RM;Henry, JR;Buchanan, SG; | Aurora A Kinase Inhibition Is Synthetic Lethal with Loss of the RB1 Tumor Suppressor Gene | Cancer discovery | 30373917 | The identification of a synthetic lethal interaction between RB1 and AURKA inhibition, and the discovery of a drug that can be dosed continuously to achieve uninterrupted inhibition of Save | Full Paper |
2019 | Mascarenhas, J;Lu, M;Kosiorek, H;Virtgaym, E;Xia, L;Sandy, L;Mesa, R;Petersen, B;Farnoud, N;Najfeld, V;Rampal, R;Dueck, A;Hoffman, R; | Oral idasanutlin in patients with polycythemia vera | Blood | 31167802 | Baseline MDM2 protein levels in the enrolled PV/ET patients and 7 normal samples (peripheral blood, n = 4; bone marrow, n = 3) purchased from ALLcells (Alameda, CA) were evaluated using monoclonal antibody staining and flow cytometric analysis.24 Bri | Full Paper |
2019 | Drissen, R;Thongjuea, S;Theilgaard-Mönch, K;Nerlov, C; | Identification of two distinct pathways of human myelopoiesis | Science immunology | 31126997 | Bone marrow samples were from AllCells or taken from healthy male volunteers at the age of 21 to 29 years old, who provided written informed consent in accordance with local Save | Full Paper |
2019 | Hu, CW;Qiu, Y;Ligeralde, A;Raybon, AY;Yoo, SY;Coombes, KR;Qutub, AA;Kornblau, SM; | A quantitative analysis of heterogeneities and hallmarks in acute myelogenous leukaemia | Nature biomedical engineering | 30988472 | was prepared as described in publications11,21. In addition to AML patient samples, each array includes the same ten cryopreserved normal bone marrow CD34+ samples (NLBM CD34+) from healthy subjects (AllCells, Alameda, CA). Mutation Analysis All mut | Full Paper |
2019 | Maekawa, T;Kato, S;Kawamura, T;Takada, K;Sone, T;Ogata, H;Saito, K;Izumi, T;Nagao, S;Takano, K;Okada, Y;Tachi, N;Teramoto, M;Horiuchi, T;Hikota-Saga, R;Endo-Umeda, K;Uno, S;Osawa, Y;Kobayashi, A;Kobayashi, S;Sato, K;Hashimoto, M;Suzu, S;Usuki, K;Morishita, S;Araki, M;Makishima, M;Komatsu, N;Kimura, F; | Increased SLAMF7high monocytes in myelofibrosis patients harboring JAK2V617F provide a therapeutic target of elotuzumab | Blood | 31270105 | The hNOG mouse was created by transplanting human cord blood (Allcells, Alameda, CA) into the NOG mouse established by backcrossing an interleukin-2R (IL-2R) γ-chain knockout Save | Full Paper |
2019 | Pang, WW;Czechowicz, A;Logan, AC;Bhardwaj, R;Poyser, J;Park, CY;Weissman, IL;Shizuru, JA; | Anti-CD117 antibody depletes normal and myelodysplastic syndrome human hematopoietic stem cells in xenografted mice | Blood | 30745302 | Normal human BM samples were purchased from AllCells (Alameda, CA). Human umbilical cord blood (UCB) samples and human BM samples from MDS patients were obtained Save | Full Paper |
2019 | Abdel-Azim, H;Dvorak, C;Bunin, N;Walters, M;Talano, J;Anderson, E;Malvar, J;Al Jassani, M;Chaudhury, S;Cairo, M;Kitko, C;Jacob, A;Monos, D;Wing, L;Pulsipher, M; | Next Generation Sequence Minimal Residual Disease (NGS-MRD) Predicts Outstanding Event Free Survival (EFS) Regardless of Hematopoietic Cell Transplantation (HCT) Preparative Approach or Graft Alpha/Beta Depletion in Children with Acute Lymphoblastic Leukemia (ALL) | Blood | MRD detected by flow cytometry (FC) or PCR has been associated with key outcomes after HCT for ALL. In a prospective multicenter trial (NCT02646839; Pediatric Blood and Marrow Transplant Consortium [PBMTC] ONC1401), we performed a planned sub-study o | Full Paper | |
2019 | Dunbar, A;Kosiorek, H;Krishnan, A;McGovern, E;Park, Y;Patel, M;Weinberg, R;Yacoub, A;Dueck, A;Mascarenhas, J;Levine, R;Hoffman, R;Rampal, R; | Identifying Cytokine Biomarkers of Response to Pegylated-Interferon Therapy in Polycythemia Vera and Essential Thrombocythemia: Correlative Analysis from the MPN-RC 111/112 Trials | Blood | Age-matched serum from healthy individuals (N=6) was obtained from AllCells TM for use as wild-type (WT) controls. Baseline cytokine samples were compared between groups of Save;Background: Pegylated Interferon-alpha (PEG-IFNa) improves hematologic | Full Paper | |
2019 | Park, SH;Lee, CM;Dever, DP;Davis, TH;Camarena, J;Srifa, W;Zhang, Y;Paikari, A;Chang, AK;Porteus, MH;Sheehan, VA;Bao, G; | Highly efficient editing of the β-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell disease | Nucleic acids research | 31147717 | K562 cells were cultured in RPMI1640 (ATCC, Cat No. 30-2001) supplemented with 10% FBS and L-glutamine. Frozen CD34+ cells from mobilized peripheral blood of normal individuals were purchased from AllCells. Peripheral blood CD34+ cells were acquired | Full Paper |
2019 | Shortridge, MD;Wille, PT;Jones, AN;Davidson, A;Bogdanovic, J;Arts, E;Karn, J;Robinson, JA;Varani, G; | An ultra-high affinity ligand of HIV-1 TAR reveals the RNA structure recognized by P-TEFb | Nucleic acids research | 30481318 | stimulate HIV-1 transcription. Twenty-four hours later, the percentage of cells expressing GFP was determined by flow cytometry as described above. Peptide uptake Frozen, de-identified human PBMCs (Allcells, Alameda, CA) were thawed and stimulated w | Full Paper |
2019 | McGrath, E;Shin, H;Zhang, L;Phue, JN;Wu, WW;Shen, RF;Jang, YY;Revollo, J;Ye, Z; | Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing | Nature communications | 31767844 | Briefly, CD34+ cells from a healthy male donor were purchased from AllCells (Alameda, CA, USA) and were cultured in StemSpan medium (STEMCELL Technologies, Vancouver, BC, Save | Full Paper |
2019 | Sachdeva, M;Busser, BW;Temburni, S;Jahangiri, B;Gautron, AS;Maréchal, A;Juillerat, A;Williams, A;Depil, S;Duchateau, P;Poirot, L;Valton, J; | Repurposing endogenous immune pathways to tailor and control chimeric antigen receptor T cell functionality | Nature communications | 31723132 | are able to integrate complex environmental inputs and to generate custom outputs in a highly regulated and specific manner. Methods Biological materials Cryopreserved human PBMCs were acquired from ALLCELLS (cat # PB006F) and used in accordance wit | Full Paper |
2019 | Gomez-Ospina, N;Scharenberg, SG;Mostrel, N;Bak, RO;Mantri, S;Quadros, RM;Gurumurthy, CB;Lee, C;Bao, G;Suarez, CJ;Khan, S;Sawamoto, K;Tomatsu, S;Raj, N;Attardi, LD;Aurelian, L;Porteus, MH; | Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type I | Nature communications | 31492863 | ck-treated samples, and 50% modification control were included. Data was analyzed using QuantaSoft (Bio-Rad). HSPC selection and culturing Human CD34+ HSPCs mobilized peripheral blood purchased from AllCells (Alameda, CA, USA) and thawed per manufac | Full Paper |
2019 | Zhang, Y;Roos, M;Himburg, H;Termini, CM;Quarmyne, M;Li, M;Zhao, L;Kan, J;Fang, T;Yan, X;Pohl, K;Diers, E;Jin Gim, H;Damoiseaux, R;Whitelegge, J;McBride, W;Jung, ME;Chute, JP; | PTPσ inhibitors promote hematopoietic stem cell regeneration | Nature communications | 31413255 | collected from the recipient mice and were analyzed for percentages of DsRed+ cells in the BM17. Human BM cultures and human BM transplantation assays Human BM mononuclear cells were purchased from AllCells. Cryopreserved human BM cells were recover | Full Paper |
2019 | Pellin, D;Loperfido, M;Baricordi, C;Wolock, SL;Montepeloso, A;Weinberg, OK;Biffi, A;Klein, AM;Biasco, L; | A comprehensive single cell transcriptional landscape of human hematopoietic progenitors | Nature communications | 31160568 | from MNCs with the human anti-CD34 MicroBeads Isolation Kit (Miltenyi Biotec) according to the manufacturer's specifications or were purchased from commercial sources (AllCells). Save | Full Paper |
2019 | Pavel-Dinu, M;Wiebking, V;Dejene, BT;Srifa, W;Mantri, S;Nicolas, CE;Lee, C;Bao, G;Kildebeck, EJ;Punjya, N;Sindhu, C;Inlay, MA;Saxena, N;DeRavin, SS;Malech, H;Roncarolo, MG;Weinberg, KI;Porteus, MH; | Gene correction for SCID-X1 in long-term hematopoietic stem cells | Nature communications | 30967552 | d to the purified AAV6 at a final concentration of 0.001%, aliquot and stored at −80 °C. CD34+ HSPCs Mobilized peripheral blood (mPB) and bone marow (BM) CD34+ HSPCs cells were purchased from AllCells (Alameda, CA, USA). Cells were thawed using pub | Full Paper |
2019 | Kimmey, SC;Borges, L;Baskar, R;Bendall, SC; | Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cells | Nature communications | 30862852 | Fresh bone marrow aspirates from two independent donors were obtained from AllCells (Alameda, CA) on the same day of collection, and immediately after receiving was placed in 37 Save | Full Paper |
2019 | Paschon, DE;Lussier, S;Wangzor, T;Xia, DF;Li, PW;Hinkley, SJ;Scarlott, NA;Lam, SC;Waite, AJ;Truong, LN;Gandhi, N;Kadam, BN;Patil, DP;Shivak, DA;Lee, GK;Holmes, MC;Zhang, L;Miller, JC;Rebar, EJ; | Diversifying the structure of zinc finger nucleases for high-precision genome editing | Nature communications | 30850604 | rer’s instructions. Target loci were then analyzed by PCR amplification followed by deep sequencing (MiSeq). Studies performed in T-cells Peripheral blood CD3 + Pan T-cells were purchased from AllCells (Alameda, CA). Transfections into activated T-c | Full Paper |
2019 | Czechowicz, A;Palchaudhuri, R;Scheck, A;Hu, Y;Hoggatt, J;Saez, B;Pang, WW;Mansour, MK;Tate, TA;Chan, YY;Walck, E;Wernig, G;Shizuru, JA;Winau, F;Scadden, DT;Rossi, DJ; | Selective hematopoietic stem cell ablation using CD117-antibody-drug-conjugates enables safe and effective transplantation with immunity preservation | Nature communications | 30728354 | als into 9 Gy, lethally irradiated, C57BL/6J (CD45.2+) wild-type recipients as previously described16. Human cord blood transplantation Single donor-derived cord blood CD34+ cells were obtained from AllCells (Alameda, CA) under local IRB-approved pr | Full Paper |
2019 | Zeng, Y;Yao, X;Liu, X;He, X;Li, L;Liu, X;Yan, Z;Wu, J;Fu, BM; | Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis | Journal of extracellular vesicles | 31258881 | o inhibit tumor vasculogenesis may serve as a novel strategy to augment the long-term efficacy of anti-angiogenic and anti-autophagic therapies for tumors. Materials and methods Cell culture HUVECs (Allcells, Shanghai, China) were cultured in HUVEC | Full Paper |
2019 | Mu, Q;Cabana-Puig, X;Mao, J;Swartwout, B;Abdelhamid, L;Cecere, TE;Wang, H;Reilly, CM;Luo, XM; | Pregnancy and lactation interfere with the response of autoimmunity to modulation of gut microbiota | Microbiome | 31311609 | Human plasma samples were purchased from AllCells (Alameda, CA). For measuring anti-DNA IgG, anti-LPS IgG, and anti-flagellin IgG, high-binding plates were coated overnight at 4 Save | Full Paper |
2019 | Li, W;Wu, D;Zhu, S;Liu, Z;Luo, B;Lu, L;Zhou, C; | Sustained release of plasmid DNA from PLLA/POSS nanofibers for angiogenic therapy | Chemical Engineering Journal | Human umbilical vein endothelial cells (HUVECs, purchased from Allcells, shanghai, China) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (P/S, Gibco) at 37 °C in 5% CO2 | Full Paper | |
2019 | Wu, HW;Sheard, MA;Malvar, J;Fernandez, GE;DeClerck, YA;Blavier, L;Shimada, H;Theuer, CP;Sposto, R;Seeger, RC; | Anti-CD105 Antibody Eliminates Tumor Microenvironment Cells and Enhances Anti-GD2 Antibody Immunotherapy of Neuroblastoma with Activated Natural Killer Cells | Clinical cancer research : an official journal of the American Association for Cancer Research | 31068371 | Human umbilical vein endothelial cells (HUVEC) were purchased from AllCells, LLC and maintained in HUVEC Basal Medium containing 10% Stimulatory Supplement from AllCells in Save | Full Paper |
2019 | Zuch de Zafra, CL;Fajardo, F;Zhong, W;Bernett, MJ;Muchhal, US;Moore, GL;Stevens, J;Case, R;Pearson, JT;Liu, S;McElroy, PL;Canon, J;Desjarlais, JR;Coxon, A;Balazs, M;Nolan-Stevaux, O; | Targeting Multiple Myeloma with AMG 424, a Novel Anti-CD38/CD3 Bispecific T-cell-recruiting Antibody Optimized for Cytotoxicity and Cytokine Release | Clinical cancer research : an official journal of the American Association for Cancer Research | 30918018 | Frozen human T cells (AllCells) or frozen peripheral blood mononuclear cells (PBMC) from humans or cynomolgus (CepheusBio) were cocultured with target cells at an effector cell (E) Save | Full Paper |
2019 | Jin, Q;Martinez, CA;Arcipowski, KM;Zhu, Y;Gutierrez-Diaz, BT;Wang, KK;Johnson, MR;Volk, AG;Wang, F;Wu, J;Grove, C;Wang, H;Sokirniy, I;Thomas, PM;Goo, YA;Abshiru, NA;Hijiya, N;Peirs, S;Vandamme, N;Berx, G;Goosens, S;Marshall, SA;Rendleman, EJ;Takahashi, YH;Wang, L;Rawat, R;Bartom, ET;Collings, CK;Van Vlierberghe, P;Strikoudis, A;Kelly, S;Ueberheide, B;Mantis, C;Kandela, I;Bourquin, JP;Bornhauser, B;Serafin, V;Bresolin, S;Paganin, M;Accordi, B;Basso, G;Kelleher, NL;Weinstock, J;Kumar, S;Crispino, JD;Shilatifard, A;Ntziachristos, P; | USP7 Cooperates with NOTCH1 to Drive the Oncogenic Transcriptional Program in T-Cell Leukemia | Clinical cancer research : an official journal of the American Association for Cancer Research | 30224337 | were maintained in DMEM medium supplemented with 10% heat-inactivated FBS, 2% penicillin/streptomycin, and 1% GlutaMAX. Human pan T cells were purchased from AllCells.com (Alameda, CA). Primary human samples were collected by collaborating institutio | Full Paper |
2019 | Zheng, B;Yu, SF;Del Rosario, G;Leong, SR;Lee, GY;Vij, R;Chiu, C;Liang, WC;Wu, Y;Chalouni, C;Sadowsky, J;Clark, V;Hendricks, A;Poon, KA;Chu, W;Pillow, T;Schutten, MM;Flygare, J;Polson, AG; | An Anti-CLL-1 Antibody-Drug Conjugate for the Treatment of Acute Myeloid Leukemia | Clinical cancer research : an official journal of the American Association for Cancer Research | 29959143 | Bone marrow mononuclear cells (BM-MNC) from healthy human donors (AllCells) were prepared by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare). CD34 + Save | Full Paper |
2019 | Castillo, J;Wu, E;Lowe, C;Srinivasan, S;McCord, R;Wagle, MC;Jayakar, S;Edick, MG;Eastham-Anderson, J;Liu, B;Hutchinson, KE;Jones, W;Stokes, MP;Tarighat, SS;Holcomb, T;Glibicky, A;Romero, FA;Magnuson, S;Huang, SA;Plaks, V;Giltnane, JM;Lackner, MR;Mounir, Z; | CBP/p300 Drives the Differentiation of Regulatory T Cells through Transcriptional and Non-Transcriptional Mechanisms | Cancer research | 31182547 | Normal human peripheral blood CD4+/CD45RA+/CD25− naïve T cells were procured from Allcells (PB009-5). Cells were isolated using Immunomagnetic CD4+ Isolation Kit, CD45RO microbeads to deplete CD45RO+ cells, and cultured using Dynabeads human T activa | Full Paper |
2019 | Levi, J;Lam, T;Goth, SR;Yaghoubi, S;Bates, J;Ren, G;Jivan, S;Huynh, TL;Blecha, JE;Khattri, R;Schmidt, KF;Jennings, D;VanBrocklin, H; | Imaging of Activated T Cells as an Early Predictor of Immune Response to Anti-PD-1 Therapy | Cancer research | 31064845 | Neutrophils and eosinophils were isolated from human whole blood (AllCells, Alameda CA). Human neutrophil and eosinophil enrichment kit (StemCell Technologies) was used to purify the cells. Neutrophils were either treated with 1uM N-formyl- L-methion | Full Paper |
2019 | Boudreaux, SP;Duren, RP;Call, SG;Nguyen, L;Freire, PR;Narayanan, P;Redell, MS;Conneely, OM; | Drug targeting of NR4A nuclear receptors for treatment of acute myeloid leukemia | Leukemia | 29884904 | rmed consent at Texas Children’s Hospital in accordance with the Declaration of Helsinki and institutional review board regulations. Normal cord blood CD34+ stem/progenitor cells were obtained from AllCells. Connectivity Map (CMAP) Analysis The NR4A | Full Paper |
2019 | Shen, JC;Kamath-Loeb, AS;Kohrn, BF;Loeb, KR;Preston, BD;Loeb, LA; | A high-resolution landscape of mutations in the BCL6 super-enhancer in normal human B cells | Proceedings of the National Academy of Sciences of the United States of America | 31748270 | Human B Cells. The CD19+ B cells were purchased from AllCells and ReachBio. Peripheral blood mononuclear cells (PBMCs) were purified from whole blood drawn from 10 healthy donors (SI Appendix, Table S2). CD19+ B cells were purified by positive select | Full Paper |
2019 | Zhang, L;Dong, X;Lee, M;Maslov, AY;Wang, T;Vijg, J; | Single-cell whole-genome sequencing reveals the functional landscape of somatic mutations in B lymphocytes across the human lifespan | Proceedings of the National Academy of Sciences of the United States of America | 30992375 | Two frozen human umbilical cord blood mononuclear cell samples were obtained from AllCells and STEMCELL Technologies. Informed consent was obtained from all the donors. The Save | Full Paper |
2019 | Ye, S;Cohen, D;Belmar, NA;Choi, D;Tan, SS;Sho, M;Akamatsu, Y;Kim, H;Iyer, R;Cabel, J;Lake, M;Song, D;Harlan, J;Zhang, C;Fang, Y;Wahl, AF;Culp, P;Hollenbaugh, D;Chao, DT; | A Bispecific Molecule Targeting CD40 and Tumor Antigen Mesothelin Enhances Tumor-Specific Immunity | Cancer immunology research | 31462409 | than 10 passages from master cell banking. Fresh human peripheral blood mononuclear cells (PBMC) from healthy donors (about 109 per donor) were purchased from AllCells. Donor virus testing results of human immunodeficiency virus, hepatitis B virus, a | Full Paper |
2019 | Gui, X;Deng, M;Song, H;Chen, Y;Xie, J;Li, Z;He, L;Huang, F;Xu, Y;Anami, Y;Yu, H;Yu, C;Li, L;Yuan, Z;Xu, X;Wang, Q;Chai, Y;Huang, T;Shi, Y;Tsuchikama, K;Liao, XC;Xia, N;Gao, GF;Zhang, N;Zhang, CC;An, Z; | Disrupting LILRB4/APOE Interaction by an Efficacious Humanized Antibody Reverses T-cell Suppression and Blocks AML Development | Cancer immunology research | 31213474 | In the coculture assay, human T cells (5 × 10 4 cells per well) isolated from health donor peripheral blood (AllCells) were placed in the lower chambers of a 96-well transwell plate. Save | Full Paper |
2019 | Newman, M; | Abstract B178: Development and preclinical efficacy characterization of a systemically administered multiple Toll-like receptor (TLR) agonist for antitumor immunotherapy | Cancer Immunology Research | PBMCs from fresh normal peripheral blood leukapheresis paks (ALLCells, Alameda, CA) were isolated using a Ficoll gradient. Ten-fold increments of 1x10/mL to 1x10^8/mL untreated Save | Full Paper | |
2019 | Sun, Y;Xiao, Y;Sun, H;Zhao, Z;Zhu, J;Zhang, L;Dong, J;Han, T;Jing, Q;Zhou, J;Jing, Z; | miR-27a regulates vascular remodeling by targeting endothelial cells' apoptosis and interaction with vascular smooth muscle cells in aortic dissection | Theranostics | 31695809 | Prime HUVECs were purchased from ALLCELLS and cultured in endothelial cell media (ALLCELLS, Emeryville, California) at 37℃ in a humidified atmosphere with 5% CO 2 . HEK 293 Save | Full Paper |
2019 | Varricchio, L;Planutis, A;Manwani, D;Jaffray, J;Mitchell, WB;Migliaccio, AR;Bieker, JJ; | Genetic disarray follows mutant KLF1-E325K expression in a congenital dyserythropoietic anemia patient | Haematologica | 30872368 | no new patient material was obtained for the present study. Mononuclear cells from the peripheral blood (PBMC) had been isolated and cryopreserved as described.35 Non-patient PBMC were purchased from AllCells (PB003F). Human erythroid massive amplif | Full Paper |
2019 | Sapienza, MR;Abate, F;Melle, F;Orecchioni, S;Fuligni, F;Etebari, M;Tabanelli, V;Laginestra, MA;Pileri, A;Motta, G;Rossi, M;Agostinelli, C;Sabattini, E;Pimpinelli, N;Truni, M;Falini, B;Cerroni, L;Talarico, G;Piccioni, R;Amente, S;Indio, V;Tarantino, G;Brundu, F;Paulli, M;Berti, E;Facchetti, F;Dellino, GI;Bertolini, F;Tripodo, C;Rabadan, R;Pileri, SA; | Blastic plasmacytoid dendritic cell neoplasm: genomics mark epigenetic dysregulation as a primary therapeutic target | Haematologica | 30381297 | RNA of 4 normal plasmacytoid dendritic cell (pDCs) samples was purchased from AllCells (Alameda, CA, US) and used for comparison. For details, see Online Supplementary Table S6 Save;BPDCN cryopreserved cutaneous biopsies, sufficient only for RNA se | Full Paper |
2019 | Yao, X;Liu, R;Liang, X;Ding, J; | Critical Areas of Proliferation of Single Cells on Micropatterned Surfaces and Corresponding Cell Type Dependence | ACS applied materials & interfaces | 30964630 | Primary rat MSCs were purchased from Allcells Tech., Shanghai, People’s Republic of China; primary mouse embryo fibroblasts (EF) were purchased from Shanghai Gaining Biological Technology Co., Ltd.; human cervix epithelial carcinoma cells (Hela) and | Full Paper |
2019 | Deng, X;Chu, X;Wang, P;Ma, X;Wei, C;Sun, C;Yang, J;Li, Y; | MicroRNA-29a-3p Reduces TNFα-Induced Endothelial Dysfunction by Targeting Tumor Necrosis Factor Receptor 1 | Molecular therapy. Nucleic acids | 31760375 | ina), and HEK293 cells (ATCC, Manassas, VA, USA) were maintained in DMEM (HyClone, South Logan, UT, USA) containing 10% fetal bovine serum (FBS; PAA Laboratories, Austria). pHUVECs were obtained from AllCells (China) and cultured incomplete medium (A | Full Paper |
2019 | Mandal, S;Prathipati, PK;Belshan, M;Destache, CJ; | A potential long-acting bictegravir loaded nano-drug delivery system for HIV-1 infection: A proof-of-concept study | Antiviral research | 30991088 | TZM-bl cells were obtained from NIH AIDS reagent program (Kappes and Wu, 2018; Kappes et al., 2015). Peripheral blood mononuclear cells (PBMCs) were obtained from commercial vendor (AllCells Inc. Alameda, CA). TZM-bl cells were maintained in complete | Full Paper |
2019 | Bongen, E;Lucian, H;Khatri, A;Fragiadakis, GK;Bjornson, ZB;Nolan, GP;Utz, PJ;Khatri, P; | Sex Differences in the Blood Transcriptome Identify Robust Changes in Immune Cell Proportions with Aging and Influenza Infection | Cell reports | 31722210 | Sample preparation and CyTOF methods have been described previously (Fragiadakis et al., 2019). Briefly, peripheral blood was obtained from 86 human adult volunteers from the Stanford Blood Center, AllCells (exempt, non-human subjects research), and | Full Paper |
2019 | Shehata, L;Maurer, DP;Wec, AZ;Lilov, A;Champney, E;Sun, T;Archambault, K;Burnina, I;Lynaugh, H;Zhi, X;Xu, Y;Walker, LM; | Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability | Cell reports | 31553901 | Biological Samples Healthy donor bone marrow mononuclear cells AllCells https://www.allcells.com/ Healthy human peripheral blood mononuclear cells Dartmouth Hitchcock Medical Center N/A | Full Paper |
2019 | Shi, D;Dong, Y;Dai, P;Gao, J;Yin, J;Xie, M; | Dentin matrix protein 1 correlates with the severity of hemorrhagic fever with renal syndrome and promotes hyper-permeability of endothelial cells infected by Hantaan virus | Microbes and infection | 30735719 | Cells were cultured in AllCells complete endothelial cell medium and incubated at 37 C with 5% CO 2 . HUVECs (passage 3 or 4) of approximately 70-80% confluence were used for all Save | Full Paper |
2019 | Muñoz, DP;Yannone, SM;Daemen, A;Sun, Y;Vakar-Lopez, F;Kawahara, M;Freund, AM;Rodier, F;Wu, JD;Desprez, PY;Raulet, DH;Nelson, PS;van 't Veer, LJ;Campisi, J;Coppé, JP; | Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging | JCI insight | 31184599 | Freshly isolated human PBMCs were maintained in supplemented RPMI media and cultured in 3% O2 conditions. Monocytes were obtained from ATCC (U934 and Thp1) or AllCells (CD36 selection; 99%pure). Purified NK, T, and DC cells were from AllCells (99%pur | Full Paper |
2019 | Morgan, H;Tseng, SY;Gallais, Y;Leineweber, M;Buchmann, P;Riccardi, S;Nabhan, M;Lo, J;Gani, Z;Szely, N;Zhu, CS;Yang, M;Kiessling, A;Vohr, HW;Pallardy, M;Aswad, F;Turbica, I; | Evaluation of in vitro Assays to Assess the Modulation of Dendritic Cells Functions by Therapeutic Antibodies and Aggregates | Frontiers in immunology | 31001248 | Wuppertal (Germany). For each site, healthy donors gave their written consent for the use of blood donation for research purposes. Also, PBMC directly isolated and frozen were purchased from vendor (AllCells, CA, USA). In each partner lab's, PBMC is | Full Paper |
2019 | Saul, L;Mair, I;Ivens, A;Brown, P;Samuel, K;Campbell, JDM;Soong, DY;Kamenjarin, N;Mellanby, RJ; | 1,25-Dihydroxyvitamin D3 Restrains CD4+ T Cell Priming Ability of CD11c+ Dendritic Cells by Upregulating Expression of CD31 | Frontiers in immunology | 30984180 | SA as per manufacturer's instructions (eBioscience, San Diego, USA). Generation of Human DC From Mobilized Peripheral Blood CD34+ Progenitor Cells CD34+ cells were obtained from a commercial source (AllCells, LLC., Alameda, CA, USA). The CD34+ cells | Full Paper |
2019 | Smith, AJ;Miller, SM;Buhl, C;Child, R;Whitacre, M;Schoener, R;Ettenger, G;Burkhart, D;Ryter, K;Evans, JT; | Species-Specific Structural Requirements of Alpha-Branched Trehalose Diester Mincle Agonists | Frontiers in immunology | 30873180 | PBMCs were obtained via leukapheresis from normal donors (AllCells, Berkeley, CA) or fresh blood was obtained from health human donors through a University of Montana Save | Full Paper |
2019 | Du, Y;Montoya, C;Orrego, S;Wei, X;Ling, J;Lelkes, PI;Yang, M; | Topographic cues of a novel bilayered scaffold modulate dental pulp stem cells differentiation by regulating YAP signalling through cytoskeleton adjustments | Cell proliferation | 31424140 | ilometer (Surfcorder SE 1700, Kosaka Labs) with a 2 μm radius tip, a spread of 2.5 mm and a speed of 0.5 mm/s. 2.2 Dental pulp stem cells culture on the scaffolds Human DPSCs were purchased from AllCells LLC. These cells were guaranteed by company t | Full Paper |
2019 | Cunin, P;Bouslama, R;Machlus, KR;Martínez-Bonet, M;Lee, PY;Wactor, A;Nelson-Maney, N;Morris, A;Guo, L;Weyrich, A;Sola-Visner, M;Boilard, E;Italiano, JE;Nigrovic, PA; | Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to platelets | eLife | 31042146 | and Schulze, 2005). Fetal liver-derived MKs were generated as described (Machlus et al., 2017). Human MK generation: mobilized peripheral blood or BM CD34 +stem/progenitor cells were purchased from AllCells. 1 × 105 cells were cultured in StemSpan me | Full Paper |
2019 | Dura-Bernal, S;Suter, BA;Gleeson, P;Cantarelli, M;Quintana, A;Rodriguez, F;Kedziora, DJ;Chadderdon, GL;Kerr, CC;Neymotin, SA;McDougal, RA;Hines, M;Shepherd, GM;Lytton, WW; | NetPyNE, a tool for data-driven multiscale modeling of brain circuits | eLife | 31025934 | ode to disk file and combines these files after the simulation has ended. After gathering, the built-in analysis functions have direct access to all the network and simulation output data via sim.net.allCells and sim.allSimData. Importing and export | Full Paper |
2019 | Frank, ND;Jones, ME;Vang, B;Coeshott, C; | Evaluation of reagents used to coat the hollow-fiber bioreactor membrane of the Quantum® Cell Expansion System for the culture of human mesenchymal stem cells | Materials science & engineering. C, Materials for biological applications | 30606590 | Healthy, fresh, adult human whole bone marrow (WBM) was purchased either from AllCells LLC (Alameda, CA) or from Lonza (Basel, Switzerland). Flow cytometry staining buffer was Save | Full Paper |
2019 | Laner-Plamberger, S;Oeller, M;Mrazek, C;Hartl, A;Sonderegger, A;Rohde, E;Strunk, D;Schallmoser, K; | Upregulation of mitotic bookmarking factors during enhanced proliferation of human stromal cells in human platelet lysate | Journal of translational medicine | 31888679 | ed from white adipose tissue (WAT) and umbilical cord (UC) after signed informed consent by the donors as described previously [26, 30, 31]. Bone marrow (BM)-derived stromal cells were purchased from AllCells, (Alameda, CA, USA, http://www.allcells.c | Full Paper |
2019 | Antebi, B;Asher, AM;Rodriguez, LA;Moore, RK;Mohammadipoor, A;Cancio, LC; | Cryopreserved mesenchymal stem cells regain functional potency following a 24-h acclimation period | Journal of translational medicine | 31464641 | Human MSCs were isolated from fresh bone-marrow mononuclear cells purchased from AllCells (Alameda, CA). The MSCs were expanded in complete culture media (CCM) consisting Save | Full Paper |
2019 | Coeshott, C;Vang, B;Jones, M;Nankervis, B; | Large-scale expansion and characterization of CD3+ T-cells in the Quantum® Cell Expansion System | Journal of translational medicine | 31391068 | g and unique dual-loop circuits. Materials and methods Preparation of peripheral blood mononuclear cells (PBMCs) Peripheral blood leukapheresis products were obtained from individual healthy donors (AllCells, LLC, Alameda, CA, HemaCare Corp, Van Nuy | Full Paper |
2019 | Shi, L;Zhong, Z;Li, X;Zhou, Y;Pan, Z; | Discovery of an Orally Available Janus Kinase 3 Selective Covalent Inhibitor | Journal of medicinal chemistry | 30615446 | Frozen PBMCs (from Allcells) were thawed in RPMI1640 (Thermo Fisher) containing 10% FBS and recovered overnight at 37 °C. The next day, cells were diluted to 1 × 106 cells/mL and seeded (500 μL) in 6-well plates. Compounds or DMSO (5 μL, serially | Full Paper |
2019 | Zoine, JT;Knight, KA;Fleischer, LC;Sutton, KS;Goldsmith, KC;Doering, CB;Spencer, HT; | Ex vivo expanded patient-derived γδ T-cell immunotherapy enhances neuroblastoma tumor regression in a murine model | Oncoimmunology | 31413905 | Commercially available healthy donor frozen PBMCs were obtained from AllCells (Alameda, CA). At the time of stem cell collection, each patient had undergone two cycles of induction Save | Full Paper |
2019 | Rodriguez, LA;Mohammadipoor, A;Alvarado, L;Kamucheka, RM;Asher, AM;Cancio, LC;Antebi, B; | Preconditioning in an Inflammatory Milieu Augments the Immunotherapeutic Function of Mesenchymal Stromal Cells | Cells | 31096722 | D) for immune- and/or inflammatory-mediated diseases. 2. Materials and Methods 2.1. Isolation of BM-MSCs and AD-MSCs Human BM-MSCs were isolated from commercially available mononuclear cells (MNCs) (AllCells LLC; Emeryville, CA, USA), as previously | Full Paper |
2019 | Youn, M;Huang, H;Chen, C;Kam, S;Wilkes, MC;Chae, HD;Sridhar, KJ;Greenberg, PL;Glader, B;Narla, A;Lin, S;Sakamoto, KM; | MMP9 inhibition increases erythropoiesis in RPS14-deficient del(5q) MDS models through suppression of TGF-β pathways | Blood advances | 31540902 | Primary human CD34 + hematopoietic stem/progenitor cells were purified from bone marrow aspirates purchased from Allcells and Stemcell Technologies. CD34 + cells were purified Save | Full Paper |
2019 | Li, C;Mathews, V;Kim, S;George, B;Hebert, K;Jiang, H;Li, C;Zhu, Y;Keesler, DA;Boelens, JJ;Dvorak, CC;Agarwal, R;Auletta, JJ;Goyal, RK;Hanna, R;Kasow, K;Shenoy, S;Smith, AR;Walters, MC;Eapen, M; | Related and unrelated donor transplantation for β-thalassemia major: results of an international survey | Blood advances | 31471325 | Full Paper | |
2019 | Yang, HM;Choi, JJ;Kim, HN;Yang, SJ;Park, SJ;Kang, C;Chung, HM;Lee, MR;Kim, SJ;Moon, SH; | Reconstituting Human Cutaneous Regeneration in Humanized Mice under Endothelial Cell Therapy | The Journal of investigative dermatology | 30393080 | The derivation of CB-EPCs was performed from CB mononuclear cells, which were purchased from AllCells (Alameda, CA). Briefly, CB mononuclear cells were seeded at a density of 4-6 × 106/mL in a collagen-coated tissue culture dish and cultured in EGM-2 | Full Paper |
2019 | Jeng, MY;Mumbach, MR;Granja, JM;Satpathy, AT;Chang, HY;Chang, ALS; | Enhancer Connectome Nominates Target Genes of Inherited Risk Variants from Inflammatory Skin Disorders | The Journal of investigative dermatology | 30315781 | Briefly, donor human peripheral blood cells were obtained from AllCells (Emeryville, CA) and were sorted based on the following markers: CD4 + CD25 − CD45RA + (naive), CD4 + Save | Full Paper |
2019 | Naipauer, J;Rosario, S;Gupta, S;Premer, C;Méndez-Solís, O;Schlesinger, M;Ponzinibbio, V;Jain, V;Gay, L;Renne, R;Chan, HL;Morey, L;Salyakina, D;Abba, M;Williams, S;Hare, JM;Goldschmidt-Clermont, PJ;Mesri, EA; | PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment | PLoS pathogens | 31881074 | n SCA-1; and (iii) the ability to differentiate into adipocytes or osteoblast-like cells. Human MSCs were isolated as described previously [39]. Briefly, BM aspirates (25-50 mL) were purchased from AllCells (Emeryville, CA) under appropriate informed | Full Paper |
2019 | Bi, W;Xu, W;Cheng, L;Xue, J;Wang, Q;Yu, F;Xia, S;Wang, Q;Li, G;Qin, C;Lu, L;Su, L;Jiang, S; | IgG Fc-binding motif-conjugated HIV-1 fusion inhibitor exhibits improved potency and in vivo half-life: Potential application in combination with broad neutralizing antibodies | PLoS pathogens | 31805154 | Env (2.5 × 104 cells) as the target cells were seeded into a 96-well plate (25 μL/well), and the mixture of a serially diluted inhibitors and 10E8 (1.5 μg/mL) was added to the culture plate. PBMC (AllCells) as effector cells (2.5 × 104 cells in 25 μL | Full Paper |
2019 | Wang, W;Liu, T;Yang, L;Ma, Y;Dou, F;Shi, L;Wen, A;Ding, Y; | Study on the multi-targets mechanism of triphala on cardio-cerebral vascular diseases based on network pharmacology | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | 31112872 | Triphala was obtained from Tibet Jinzhu Along Tibetan Medicine Co. Ltd. (Tibet, China). The Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from AllCells (Emeryville, CA, USA). Primary antibodies against β-actin, PTGS2, MMP9 and IL6 we | Full Paper |
2019 | Acosta, SA;Lee, JY;Nguyen, H;Kaneko, Y;Borlongan, CV; | Endothelial Progenitor Cells Modulate Inflammation-Associated Stroke Vasculome | Stem cell reviews and reports | 30739275 | linded to the treatment condition. Cell Culture Procedures EPC Differentiation from Human Bone Marrow Mesenchymal Stem Cells Frozen human bone marrow CD34+ mesenchymal stem cells were purchased from ALLCELLS (Catalog# ABM017F; Alameda, CA). After th | Full Paper |
2019 | Meehan, S;Kolyagin, GA;Parks, D;Youngyunpipatkul, J;Herzenberg, LA;Walther, G;Ghosn, EEB;Orlova, DY; | Automated subset identification and characterization pipeline for multidimensional flow and mass cytometry data clustering and visualization | Communications biology | 31240267 | Fresh bone marrow mononuclear cells from adult healthy donors were obtained commercially from AllCells, LLC (Quincy, MA, Cat # ABM024). Data was collected for about 0.5-1.0 × 10 Save | Full Paper |
2019 | Guo, X;Yuan, Z;Xu, Y;Zhao, X;Fang, Z;Yuan, WE; | A Low-Molecular-Weight Polyethylenimine/pDNA-VEGF Polyplex System Constructed in a One-Pot Manner for Hindlimb Ischemia Therapy | Pharmaceutics | 30965617 | Aldrich. VEGF-A (1239 bp) pDNA encoding GFP was constructed by Bioroot Biology (Shanghai, China). 2.2. Cell Culture Human umbilical vein endothelial cells (HUVECs, primary cells) were purchased from ALLCELLS (Shanghai, China) and cultured in RPMI-16 | Full Paper |
2019 | Zhang, M;Xu, Y;Jiang, L; | Irisin attenuates oxidized low-density lipoprotein impaired angiogenesis through AKT/mTOR/S6K1/Nrf2 pathway | Journal of cellular physiology | 30942905 | Commercial HUVECs were obtained from Allcells (Shanghai, China). Cells were seeded in DMEM/F12 medium with 20% FBS (Gibco), 60 μg/ml endothelial cell growth supplement (BD, San Diego, CA) and maintained at 37°C in a CO2 incubator. The HMEC-1 cells we | Full Paper |
2019 | Wang, J;Fei, K;Jing, H;Wu, Z;Wu, W;Zhou, S;Ni, H;Chen, B;Xiong, Y;Liu, Y;Peng, B;Yu, D;Jiang, H;Liu, J; | Durable blockade of PD-1 signaling links preclinical efficacy of sintilimab to its clinical benefit | mAbs | 31402780 | × MSD read buffer with surfactant (R92TC-1, MSD) was added into each well. The electrochemiluminescent signal was then measured on the MSD SQ120 instrument. In vitro PD-1 receptor occupancy PBMCs (AllCells, Alameda, California, USA) were activated b | Full Paper |
2019 | Ovacik, AM;Li, J;Lemper, M;Danilenko, D;Stagg, N;Mathieu, M;Ellerman, D;Gupta, V;Kalia, N;Nguy, T;Plaks, V;David Johnson, C;Wang, W;Brumm, J;Fine, B;Junttila, T;Lin, K;Carter, PJ;Prabhu, S;Spiess, C;Kamath, AV; | Single cell-produced and in vitro-assembled anti-FcRH5/CD3 T-cell dependent bispecific antibodies have similar in vitro and in vivo properties | mAbs | 30550367 | 100 (number of live target cells without TDB - number of live target cells with TDB)/number of live target cells without TDB. Human BM aspirates from healthy donors (n = 2, male) (procured from AllCells, ABM001-1) were diluted in phosphate-buffered | Full Paper |
2019 | Wang, H;He, H;Yang, C; | miR-342 suppresses the proliferation and invasion of acute myeloid leukemia by targeting Naa10p | Artificial cells, nanomedicine, and biotechnology | 31496296 | The MNC normal control samples were isolated from normal BM cells purchased from AllCells, LLC (Emeryville, CA) by use of NycoPrep 1.077 A (Axis-Shield, Oslo, Norway) according Save | Full Paper |
2019 | Chang, AY;Hsu, E;Patel, J;Li, Y;Zhang, M;Iguchi, H;Rogoff, HA; | Evaluation of Tumor Cell-Tumor Microenvironment Component Interactions as Potential Predictors of Patient Response to Napabucasin | Molecular cancer research : MCR | 31043490 | pSTAT3 is a potential biomarker for patient response to the anticancer drug napabucasin. Visual Overview: http://mcr.aacrjournals.org/content/molcanres/17/7/1429/F1.large.jpg . Save | Full Paper |
2019 | Marklein, RA;Klinker, MW;Drake, KA;Polikowsky, HG;Lessey-Morillon, EC;Bauer, SR; | Morphological profiling using machine learning reveals emergent subpopulations of interferon-γ-stimulated mesenchymal stromal cells that predict immunosuppression | Cytotherapy | 30503100 | Human bone marrow-derived MSCs were obtained from 11 different donors purchased from Lonza and AllCells at passage two (see Supplementary Table 1 for donor specifications). A Save | Full Paper |
2019 | Sei, J;Moses, B;Becker, A;Kim, M;Kaur, N;Vemuri, M;Civin, C; | StemPro HSC expansion medium (Prototype) supports superior expansion of human hematopoietic stem-progenitor cells | Cytotherapy | Single donor, cord-blood derived, CD34+ cells obtained from AllCells were thawed and cultured in CTS StemPro HSC expansion media containing SCF, FLT3, TPO, IL3 and IL6 growth Save | Full Paper | |
2019 | Kou, X;Sun, Y;Li, S;Bian, W;Liu, Z;Zhang, D;Jiang, J; | Pharmacology Study of the Multiple Angiogenesis Inhibitor RC28-E on Anti-Fibrosis in a Chemically Induced Lung Injury Model | Biomolecules | 31652997 | The human skin fibroblast cell line Detroit 551 was purchased from AllCells (Shanghai, China) and maintained in MEM medium (Gibco, Grand Island, NY, USA) supplemented with 10% Save | Full Paper |
2019 | Zhang, Y;Yu, S;Tuazon, JP;Lee, JY;Corey, S;Kvederis, L;Kingsbury, C;Kaneko, Y;Borlongan, CV; | Neuroprotective effects of human bone marrow mesenchymal stem cells against cerebral ischemia are mediated in part by an anti-apoptotic mechanism | Neural regeneration research | 30632499 | Bcl-2 antibody was 58, 117, or 235 ng/mL. Both hMSC and Bcl-2 doses were based on pilot studies demonstrating their potencies. Cryopreserved human bone marrow CD34+ cells (hBM34+) were purchased from AllCells (Alameda, CA, USA). The exposure time of | Full Paper |
2019 | Weisgrau, KL;Vosler, LJ;Pomplun, NL;Hayes, JM;Simmons, HA;Friedrichs, KR;Rakasz, EG; | Neutrophil progenitor populations of rhesus macaques | Journal of leukocyte biology | 30395351 | We purchased de-identified, freshly obtained human bone marrow and matching blood samples from 18-65 years old healthy individuals from AllCells LLC (Boston, USA), or Lonza Inc. (Mapleton, IL, USA). The donors signed a procedure-specific consent form | Full Paper |
2019 | Cao, L;Weetall, M;Trotta, C;Cintron, K;Ma, J;Kim, MJ;Furia, B;Romfo, C;Graci, JD;Li, W;Du, J;Sheedy, J;Hedrick, J;Risher, N;Yeh, S;Qi, H;Arasu, T;Hwang, S;Lennox, W;Kong, R;Petruska, J;Moon, YC;Babiak, J;Davis, TW;Jacobson, A;Almstead, NG;Branstrom, A;Colacino, JM;Peltz, SW; | Targeting of Hematologic Malignancies with PTC299, A Novel Potent Inhibitor of Dihydroorotate Dehydrogenase with Favorable Pharmaceutical Properties | Molecular cancer therapeutics | 30352802 | AML cells, purchased from AllCells (January 2017), Inc and Champions Oncology, Inc (April 2017), were cultured in StemSpan medium (Stemcell Technologies, Vancouver). Cell lines in culture were routinely verified once per month to be mycoplasma free a | Full Paper |
2019 | Boothello, RS;Patel, NJ;Sharon, C;Abdelfadiel, EI;Morla, S;Brophy, DF;Lippman, HR;Desai, UR;Patel, BB; | A Unique Nonsaccharide Mimetic of Heparin Hexasaccharide Inhibits Colon Cancer Stem Cells via p38 MAP Kinase Activation | Molecular cancer therapeutics | 30337351 | The cells were resuspended in 10 mL of Iscove's modified Dulbecco's medium (2% FBS media; Allcells) and mixed with Methyl cellulose (R&D systems), and 1.1 mL of the final cell Save | Full Paper |
2019 | Suhito, IR;Kang, ES;Kim, DS;Baek, S;Park, SJ;Moon, SH;Luo, Z;Lee, D;Min, J;Kim, TH; | High density gold nanostructure composites for precise electrochemical detection of human embryonic stem cells in cell mixture | Colloids and surfaces. B, Biointerfaces | 31082776 | For the comparison, cryopreserved hCB-EPCs were purchased from AllCells, LLC (California, USA) [26,27]. The hCB-EPCs were thawed in a 37 °C warm bath for 2 min and washed twice with 100 μl DNase (Sigma-Aldrich) in 10 ml PBS. Cells were cultured on a | Full Paper |
2019 | Xu, P;Ganaie, SS;Wang, X;Wang, Z;Kleiboeker, S;Horton, NC;Heier, RF;Meyers, MJ;Tavis, JE;Qiu, J; | Endonuclease Activity Inhibition of the NS1 Protein of Parvovirus B19 as a Novel Target for Antiviral Drug Development | Antimicrobial agents and chemotherapy | 30530599 | Primary human CD34+ hematopoietic stem cells (HSCs) were isolated from the bone marrow of a healthy human donor. We purchased them from AllCells LLC (Alameda, CA), and B19V-containng plasma samples were obtained from ViraCor Eurofins Laboratories (Le | Full Paper |
2019 | Patel, PR;Senyuk, V;Rodriguez, NS;Oh, AL;Bonetti, E;Mahmud, D;Barosi, G;Mahmud, N;Rondelli, D; | Synergistic Cytotoxic Effect of Busulfan and the PARP Inhibitor Veliparib in Myeloproliferative Neoplasms | Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation | 30615982 | Normal CD34 + cells used as healthy controls were obtained from apheresis collection or cord blood (AllCells, Alameda, CA). Mononuclear cells were subsequently obtained by Save | Full Paper |
2019 | Campos, JM;Sousa, AC;Caseiro, AR;Pedrosa, SS;Pinto, PO;Branquinho, MV;Amorim, I;Santos, JD;Pereira, T;Mendonça, CM;Afonso, A;Atayde, LM;Maurício, AC; | Dental pulp stem cells and Bonelike® for bone regeneration in ovine model | Regenerative biomaterials | 30740242 | onmental SEM with X-ray microanalysis and electron backscattered diffraction analysis: Quanta 400 FEG ESEM/EDAX Genesis X4M . hMSC preparation Cell culture and maintenance hDPSCs were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01) | Full Paper |
2019 | Dai, L;Hu, W;Yang, Z;Chen, D;He, B;Chen, Y;Zhou, L;Xie, H;Wu, J;Zheng, S; | Upregulated expression of HOXB7 in intrahepatic cholangiocarcinoma is associated with tumor cell metastasis and poor prognosis | Laboratory investigation; a journal of technical methods and pathology | 30664713 | Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Shanghai, China; allcells.biomart.cn) and maintained in DMEM supplemented with 10% fetal bovine Save | Full Paper |
2019 | Sachdeva, M;Duchateau, P;Depil, S;Poirot, L;Valton, J; | Granulocyte-macrophage colony-stimulating factor inactivation in CAR T-cells prevents monocyte-dependent release of key cytokine release syndrome mediators | The Journal of biological chemistry | 30804212 | Cryopreserved human PBMCs were acquired from ALLCELLS (catalog no. PB006F), and human monocytes were acquired from STEMCELL Technologies (catalog no. 70035.1). Both PBMCs and monocytes were cultured in X-vivo-15 media (Lonza, catalog no. BE04-418Q), | Full Paper |
2019 | Yamamoto, R;Xu, Y;Ikeda, S;Sumida, K;Tanaka, H;Hozumi, K;Takaori-Kondo, A;Minato, N; | Thymic Development of a Unique Bone Marrow-Resident Innate-like T Cell Subset with a Potent Innate Immune Function | Journal of immunology (Baltimore, Md. : 1950) | 31085589 | Human BM mononuclear cells from healthy donors were purchased from AllCells (Alameda, CA). T cells, B cells, myeloid cells, and dendritic cells were isolated from BM cells by Save | Full Paper |
2019 | Nikitin, PA;Rose, EL;Byun, TS;Parry, GC;Panicker, S; | C1s Inhibition by BIVV009 (Sutimlimab) Prevents Complement-Enhanced Activation of Autoimmune Human B Cells In Vitro | Journal of immunology (Baltimore, Md. : 1950) | 30635392 | Primary PBMC from de-identified patients with rheumatoid arthritis were purchased from AllCells. No therapeutic modalities were used as exclusion criteria for samples from patients Save | Full Paper |
2019 | Wu, J;Chen, L;Wang, R;Song, Z;Shen, Z;Zhao, Y;Huang, S;Lin, Z; | Exosomes Secreted by Stem Cells from Human Exfoliated Deciduous Teeth Promote Alveolar Bone Defect Repair through the Regulation of Angiogenesis and Osteogenesis | ACS biomaterials science & engineering | 33405738 | (FBS; Gibco), 100 U/mL penicillin G, as well as 100 mg/mL streptomycin. HUVECs were bought from ALLCELLS (Shanghai, China) and expanded in endothelial cell growth medium (EGM). BMSCs were harvested from the femoral bones of SD rats. All experimental | Full Paper |
2019 | Huang, YJ;Lee, CY;Cao, J;Lee, HS;Chang, CH;Chen, PD;Wu, YM; | Therapeutic Potential of Plasma Proteins Derived from Umbilical Cord Blood for Acute Liver Failure | Molecular pharmaceutics | 30698974 | There are very limited clinically viable treatment options for acute liver failure, a life-threatening condition that rapidly progresses to loss of liver function. In this study, we aim to evaluate Save | Full Paper |
2019 | Seo, A;Gulsuner, S;Pierce, S;Ben-Harosh, M;Shalev, H;Walsh, T;Krasnov, T;Dgany, O;Doulatov, S;Tamary, H;Shimamura, A;King, MC; | Inherited thrombocytopenia associated with mutation of UDP-galactose-4-epimerase (GALE) | Human molecular genetics | 30247636 | CD34+ cells from human cord blood were purchased from AllCells. Thawed cells were expanded for 4 h at 37C in tissue culture incubator with X-VIVO10 media (Lonza) supplemented Save | Full Paper |
2019 | Moore, N;Chevillet, JR;Healey, LJ;McBrine, C;Doty, D;Santos, J;Teece, B;Truslow, J;Mott, V;Hsi, P;Tandon, V;Borenstein, JT;Balestrini, J;Kotz, K; | A Microfluidic Device to Enhance Viral Transduction Efficiency During Manufacture of Engineered Cellular Therapies | Scientific reports | 31641163 | Both Research Blood Components and AllCells obtain IRB approved consent from all donors giving permission to collect their blood or blood product and use or sell it for research Save | Full Paper |
2019 | Fischer, CL;Bates, AM;Lanzel, EA;Guthmiller, JM;Johnson, GK;Singh, NK;Kumar, A;Vidva, R;Abbasi, T;Vali, S;Xie, XJ;Zeng, E;Brogden, KA; | Computational Models Accurately Predict Multi-Cell Biomarker Profiles in Inflammation and Cancer | Scientific reports | 31350446 | experiments, GE KER were suspended at 1 × 105 viable cells/ml in Lymphocyte Growth Medium-3 (LGM-3, Lonza Walkersville, Inc., Walkersville, MD). Human monocyte-derived immature myeloid DC (DC, AllCells, Alameda, CA) were positively identified by flo | Full Paper |
2019 | Laner-Plamberger, S;Oeller, M;Poupardin, R;Krisch, L;Hochmann, S;Kalathur, R;Pachler, K;Kreutzer, C;Erdmann, G;Rohde, E;Strunk, D;Schallmoser, K; | Heparin Differentially Impacts Gene Expression of Stromal Cells from Various Tissues | Scientific reports | 31076619 | ed by the ethical committee of federal state of Salzburg. All donors of BM, WAT and UC signed an informed consent concerning the research use of materials. Additionally, BM samples were obtained from AllCells, (Alameda, CA, USA, www.allcells.com/stat | Full Paper |
2019 | Yea, K;Jones, TM;Jung, D;Shin, S;Arlian, BM;Han, KH;Zha, Z;Kim, MS;Oh, YS;Zhang, H;Lerner, RA; | An agonist antibody prefers relapsed AML for induction of cells that kill each other | Scientific reports | 30837591 | population studied here is much larger than that simply defined by CD34+ and CD38+ cells. Methods Culture of AML cells Human AML cells were isolated from patients and frozen at −80 °C (ABM010, AllCells). The informed consent was obtained from all pa | Full Paper |
2019 | Das, P;Panda, SK;Agarwal, B;Behera, S;Ali, SM;Pulse, ME;Solomkin, JS;Opal, SM;Bhandari, V;Acharya, S; | Novel Chitohexaose Analog Protects Young and Aged mice from CLP Induced Polymicrobial Sepsis | Scientific reports | 30814582 | mg/kg to young adult mice and 25 mg/kg to aged mice in a 0.2 mL volume near the dorsal scapular region. Cell lines Human peripheral blood mononuclear cells (HPBMCs) were purchased from LeukoPaK (AllCells); 0.5 × 106 cells/well were seeded in 96-well | Full Paper |
2019 | Kizilyer, A;Singh, MV;Singh, VB;Suwunnakorn, S;Palis, J;Maggirwar, SB; | Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis | Scientific reports | 30808933 | CD34+ cells derived from BM or umbilical cord blood were purchased from Allcells (Emeryville, CA, USA), and differentiated as previously described 48 . For inducing MK progenitor ( Save | Full Paper |
2019 | Zarriello, S;Neal, EG;Kaneko, Y;Borlongan, CV; | T-Regulatory Cells Confer Increased Myelination and Stem Cell Activity after Stroke-Induced White Matter Injury | Journal of clinical medicine | 31010132 | Purified BMSCs were obtained and stored in aliquots in dry ice (AllCells, Almeda, CA, USA). After the cells reached confluence, they were subjected to an oxygen-glucose deprivation Save | Full Paper |
2019 | Hu, B;Zou, Y;Zhang, L;Tang, J;Niedermann, G;Firat, E;Huang, X;Zhu, X; | Nucleofection with Plasmid DNA for CRISPR/Cas9-Mediated Inactivation of Programmed Cell Death Protein 1 in CD133-Specific CAR T Cells | Human gene therapy | 29706119 | Frozen peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from ALLCELLS (PB005F). The tumor cell lines used in this study were generated based on a Save | Full Paper |
2019 | Bednar, KJ;Nycholat, CM;Rao, TS;Paulson, JC;Fung-Leung, WP;Macauley, MS; | Exploiting CD22 To Selectively Tolerize Autoantibody Producing B-Cells in Rheumatoid Arthritis | ACS chemical biology | 30835424 | Frozen peripheral blood mononuclear cells (PBMCs) were obtained through AllCells (Alameda, CA) or BioreclamationIVT (Chestertown, MD) as per institutional protocols for human sample acquisition. Cells were thawed in accordance with the manufacture’s | Full Paper |
2019 | Schott, JW;León-Rico, D;Ferreira, CB;Buckland, KF;Santilli, G;Armant, MA;Schambach, A;Cavazza, A;Thrasher, AJ; | Enhancing Lentiviral and Alpharetroviral Transduction of Human Hematopoietic Stem Cells for Clinical Application | Molecular therapy. Methods & clinical development | 31338385 | lus HSC Brew GMP supplement (Miltenyi Biotec, Surrey, UK) additionally supplemented with 2% human albumin. For the GMP experiment, HD CD34+ HSPCs were isolated from a fresh mobilized leukapheresis (AllCells, Alameda, CA, USA) following standard immu | Full Paper |
2019 | Ellison, SM;Liao, A;Wood, S;Taylor, J;Youshani, AS;Rowlston, S;Parker, H;Armant, M;Biffi, A;Chan, L;Farzaneh, F;Wynn, R;Jones, SA;Heal, P;Gaspar, HB;Bigger, BW; | Pre-clinical Safety and Efficacy of Lentiviral Vector-Mediated Ex Vivo Stem Cell Gene Therapy for the Treatment of Mucopolysaccharidosis IIIA | Molecular therapy. Methods & clinical development | 31044143 | CD34 + cells were positively selected from two pooled PB mononuclear cell (PBMC) apheresis units (Allcells, USA) using the Miltenyi CliniMACSPlus instrument and reagents Save;00 for a further 24 hrs prior to seeding in CFU assay. Large-Scale Trans | Full Paper |
2019 | Liu, W;Zhang, C;Wang, L;Huang, X;Zhang, J;He, Y;Chen, L;Li, J; | Successful reversal of ovarian hyperstimulation syndrome in a mouse model by rapamycin, an mTOR pathway inhibitor | Molecular human reproduction | 31329230 | HUVEC were purchased from ALLCELLS and seeded on micropore inserts (pore size 0.4 μm, Corning) in a density of 3 × 10 5 cells per insert and cultured overnight with or without 40 Save | Full Paper |
2019 | Cheng, Y;Dai, X;Yang, T;Zhang, N;Liu, Z;Jiang, Y; | Low Long Noncoding RNA Growth Arrest-Specific Transcript 5 Expression in the Exosomes of Lung Cancer Cells Promotes Tumor Angiogenesis | Journal of oncology | 31186629 | ells were purchased from the Chinese Academy of Sciences Type Culture Collections Cell Bank (Shanghai, China). The primary human umbilical vein endothelial cells (HUVECs) were purchased from Shanghai AllCells Biotechnology Company (Shanghai, China). | Full Paper |
2019 | Kim, CH;Lim, CY;Lee, JH;Kim, KC;Ahn, JY;Lee, EJ; | Human Embryonic Stem Cells-Derived Mesenchymal Stem Cells Reduce the Symptom of Psoriasis in Imiquimod-Induced Skin Model | Tissue engineering and regenerative medicine | 30815354 | The PBMCs were purchased from AllCells, LLC. The hE-MSCs were inactivated by treatment with 10 μg/mL mitomycin-C (Sigma-Aldrich, St. Louis, MO, USA) for 1 h at 37 C. Save | Full Paper |
2019 | Geldenhuys, WJ;Nair, RR;Piktel, D;Martin, KH;Gibson, LF; | The MitoNEET Ligand NL-1 Mediates Antileukemic Activity in Drug-Resistant B-Cell Acute Lymphoblastic Leukemia | The Journal of pharmacology and experimental therapeutics | 31010844 | Primary CD3 + T cells, CD19 + B cells, peripheral blood mononuclear cells, and bone marrow mononuclear cells were purchased from AllCells (Allcells.com) and maintained in Save | Full Paper |
2019 | Xu, L;Zhao, G;Zhu, H;Wang, S;Sun, A;Zou, Y;Ge, J; | Peroxisome Proliferator-Activated Receptor-γ Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p | PPAR research | 31354796 | Quantitative analysis of stained lipid area was performed by a blinded observer using Leica QWin V3 software. 2.3. In Vitro HUVECs Culture and Cell Immunostaining Primary HUVECs were purchased from AllCells (Shanghai, China) and cultured in low-gluc | Full Paper |
2019 | Zhao, S;Molina, A;Yu, A;Hanson, J;Cheung, H;Li, X;Natkunam, Y; | High frequency of CD74 expression in lymphomas: implications for targeted therapy using a novel anti-CD74-drug conjugate | The journal of pathology. Clinical research | 30191677 | egression analysis with one site‐specific binding equation on GraphPad Prism (La Jolla, CA, USA). Whole bone marrow (BM) aspirates (3 ml each) from five healthy human donors was obtained through AllCells, Inc. (Alameda, CA, USA). FACS binding on BM | Full Paper |
2019 | Xia, F;Yang, C;Wang, Y;Youcef-Toumi, K;Reuter, C;Ivanov, T;Holz, M;Rangelow, IW; | Lights Out! Nano-Scale Topography Imaging of Sample Surface in Opaque Liquid Environments with Coated Active Cantilever Probes | Nanomaterials (Basel, Switzerland) | 31337145 | Immersion test for whole blood sample with Ethylenediaminetetraacetic acid (EDTA) anti-coagulation treatment from AllCells LLC was also conducted. The coated probe worked well at Save | Full Paper |
2019 | Yu, Y;Yoo, SM;Park, HH;Baek, SY;Kim, YJ;Lee, S;Kim, YL;Seo, KW;Kang, KS; | Preconditioning with interleukin-1 beta and interferon-gamma enhances the efficacy of human umbilical cord blood-derived mesenchymal stem cells-based therapy via enhancing prostaglandin E2 secretion and indoleamine 2,3-dioxygenase activity in dextran sulfate sodium-induced colitis | Journal of tissue engineering and regenerative medicine | 31293088 | 2.7 Isolation of CD4+ T cells with magnetic-activated cell sorting (MACS) CD4+ T cells were purified from PBMCs (ALLCells, Alameda, CA, USA) using MACS (Miltenyi Biotec, Bergisch Gladbach, Germany) as previously discribed (Hong et al., 2017) | Full Paper |
2019 | Yin, Y;Guo, J;Teng, F;Yu, L;Jiang, Y;Xie, K;Jiang, M;Fang, J; | Preparation of a Novel One-Armed Anti-c-Met Antibody with Antitumor Activity Against Hepatocellular Carcinoma | Drug design, development and therapy | 31849449 | bovine serum (FBS, qualified, Australia#10099-141; Gibco Co., Ltd) in a humidified incubator in the presence of 5% CO2 at 37°C. Human umbilical vein endothelial cells (HUVECs) were obtained from the Allcells Co., Ltd. (Shanghai, China). The cells wer | Full Paper |
2019 | Kono, M;Matsuhiroya, S;Nakazawa, F;Kaido, M;Wada, A;Tomiyama, Y; | Morphological and optical properties of human immature platelet-enriched population produced in immunodeficient mice | Platelets | 30059642 | The animals were fed a pellet chow and sterilized tap water ad libitum. Human CD34-positive cells (1 × 105) from cord blood (AllCells, CA, USA) were transplanted through the tail vein after irradiation with 2.5 Gy. Eltrombopag at 25 mg/kg body weight | Full Paper |
2019 | Gangolli, RA;Devlin, SM;Gerstenhaber, JA;Lelkes, PI;Yang, M; | A Bilayered Poly (Lactic-Co-Glycolic Acid) Scaffold Provides Differential Cues for the Differentiation of Dental Pulp Stem Cells | Tissue engineering. Part A | 29984629 | Human DPSCs were purchased from AllCells LLC (Emeryville, CA), and cultured in a basal medium comprised of 85% MEM Alpha (Gibco ThermoFisher Scientific, Waltham, MA) supplemented with 15% fetal bovine serum (Stem Cell qualified; ThermoFisher Scientif | Full Paper |
2019 | Huang, L;Tan, J;Li, W;Zhou, L;Liu, Z;Luo, B;Lu, L;Zhou, C; | Functional polyhedral oligomeric silsesquioxane reinforced poly(lactic acid) nanocomposites for biomedical applications | Journal of the mechanical behavior of biomedical materials | 30500698 | The sterilization step of samples was similar to above films. HUVECs (purchased from Allcells, shanghai, China) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin (P/S, Gibco) | Full Paper |
2019 | Cackowski, FC;Wang, Y;Decker, JT;Sifuentes, C;Weindorf, S;Jung, Y;Wang, Y;Decker, AM;Yumoto, K;Szerlip, N;Buttitta, L;Pienta, KJ;Morgan, TM;Taichman, RS; | Detection and isolation of disseminated tumor cells in bone marrow of patients with clinically localized prostate cancer | The Prostate | 31449673 | Normal marrow samples were purchased from AllCells (Alameda, CA), drawn from the posterior superior iliac crest of paid donors, transferred to heparinized tubes and shipped Save | Full Paper |
2019 | Zheng, Q;Zhang, X;Yang, H;Xie, J;Xie, Y;Chen, J;Yu, C;Zhong, C; | Internal Ribosome Entry Site Dramatically Reduces Transgene Expression in Hematopoietic Cells in a Position-Dependent Manner | Viruses | 31597367 | anassas, VA, USA). Human embryonic kidney cell line 293T was purchased from the Institute of Biochemistry Cell Biology (Shanghai, China). The CD34+ hematopoietic stem cells (HSCs) were purchased from ALLCELLS (Alameda, CA, USA). The hepatocellular ca | Full Paper |
2019 | Zhao, H;Chen, L;Zhong, G;Huang, Y;Zhang, X;Chu, C;Chen, L;Wang, M; | Titanium Dioxide Nanoparticles Induce Mitochondrial Dynamic Imbalance and Damage in HT22 Cells | Journal of Nanomaterials | HT22 cells were from AllCells (Shanghai Biological Technology Co. Ltd., Shanghai, China). We carried out some experiments in the BE(2)C cell line, which is a human neuroblastoma cell line. The cells were planted in Dulbecco’s minimum essential medium | Full Paper | |
2019 | Liang, YR;Zhang, T;Jia, P;Xu, XL;Fang, Y;Ding, XQ; | Interaction between bone marrow-derived dendritic cells and miR-21 of tubular renal epithelial cells under hypoxia | European review for medical and pharmacological sciences | 30840288 | cluster of differentiation 86), MHCII (major histocompatibility complex class II molecules), and CCR7 (eBioscience, Frankfurt, Germany). Primary Murine Renal TEC Culture Primary renal TECs were bought from AllCells (Allcells, LLC, Berkeley, CA, USA) | Full Paper |
2019 | Youngblood, BA;Brock, EC;Leung, J;Falahati, R;Bryce, PJ;Bright, J;Williams, J;Shultz, LD;Greiner, DL;Brehm, MA;Bebbington, C;Tomasevic, N; | AK002, a Humanized Sialic Acid-Binding Immunoglobulin-Like Lectin-8 Antibody that Induces Antibody-Dependent Cell-Mediated Cytotoxicity against Human Eosinophils and Inhibits Mast Cell-Mediated Anaphylaxis in Mice | International archives of allergy and immunology | 31401630 | Purified human natural killer (NK) cells (AllCells) were washed with culture medium and added at 450,000 cells/well (effector: target cell ratio 9:1). Plates were incubated for 4 h at 37C Save | Full Paper |
2019 | Gautam, A;Donohue, D;Hoke, A;Miller, SA;Srinivasan, S;Sowe, B;Detwiler, L;Lynch, J;Levangie, M;Hammamieh, R;Jett, M; | Investigating gene expression profiles of whole blood and peripheral blood mononuclear cells using multiple collection and processing methods | PloS one | 31809517 | and the PAXgene blood RNA tubes were immediately frozen, one set of triplicate EDTA tubes was stored at 4C, and the remaining tubes received further processing at the AllCells, Save | Full Paper |
2019 | Caseiro, AR;Santos Pedrosa, S;Ivanova, G;Vieira Branquinho, M;Almeida, A;Faria, F;Amorim, I;Pereira, T;Maurício, AC; | Mesenchymal Stem/ Stromal Cells metabolomic and bioactive factors profiles: A comparative analysis on the umbilical cord and dental pulp derived Stem/ Stromal Cells secretome | PloS one | 31774816 | ngiogenesis. 2. Materials and methods 2.1. Human Mesenchymal Stem/ Stromal Cells characterization and conditioning protocol Umbilical Cord -MSCs [PromoCellTM (C-12971; Lot No. 1112304.2)] and DPSCs [AllCells, LLC (DP0037F, Lot No. DPSC090411-01)] we | Full Paper |
2019 | Manshouri, T;Verstovsek, S;Harris, DM;Veletic, I;Zhang, X;Post, SM;Bueso-Ramos, CE;Estrov, Z; | Primary myelofibrosis marrow-derived CD14+/CD34- monocytes induce myelofibrosis-like phenotype in immunodeficient mice and give rise to megakaryocytes | PloS one | 31569199 | nstitutional Review Board (MDACC IRB1) approved written informed consent had been obtained. The patients’ clinical data are provided in S1 Table. As controls, normal BM aspirates were obtained from AllCells (Alameda, CA). Low-density cells were fract | Full Paper |
2019 | Singer, JW;Al-Fayoumi, S;Taylor, J;Velichko, S;O'Mahony, A; | Comparative phenotypic profiling of the JAK2 inhibitors ruxolitinib, fedratinib, momelotinib, and pacritinib reveals distinct mechanistic signatures | PloS one | 31560729 | eline Cell Technologies and Cell Applications. Peripheral blood mononuclear cells (PBMCs) and positively selected primary normal human CD19+ B cells, CD4+ T cells, and CD14+ monocytes were purchased (AllCells, Emeryville, CA) or were isolated in-hous | Full Paper |
2019 | Gunawardena, TNA;Masoudian, Z;Rahman, MT;Ramasamy, TS;Ramanathan, A;Abu Kasim, NH; | Dental derived stem cell conditioned media for hair growth stimulation | PloS one | 31042749 | ulp stem cells obtained from human deciduous teeth (SHED) in comparison to CM produced using HFSCs. Methodology Cell culturing of SHED and HFSCs SHED and HFSCs Cells at Passage 1 were purchased from AllCells, USA and ICELLTIS, Labege, France respect | Full Paper |
2019 | Mamontov, P;Eberwine, RA;Perrigoue, J;Das, A;Friedman, JR;Mora, JR; | A negative role for the interleukin-2-inducible T-cell kinase (ITK) in human Foxp3+ TREG differentiation | PloS one | 31022269 | or pharmacological targeting of specific Tec kinases in inflammatory and autoimmune disease. Materials and methods Cell culture Naïve CD4+ CD45RA+ CD25- peripheral blood T cells were purchased from AllCells (Alameda, CA). For Th0 non-polarized condi | Full Paper |
2019 | Rytlewski, J;Deng, S;Xie, T;Davis, C;Robins, H;Yusko, E;Bienkowska, J; | Model to improve specificity for identification of clinically-relevant expanded T cells in peripheral blood | PloS one | 30870493 | expansion, have broad clinical value. Methods Training cohort Whole blood samples collected from 10 healthy donors at three time points with 2-week intervals between collections were purchased from AllCells, LLC (Emeryville, CA). Among the 10 donors | Full Paper |
2019 | Yoshida, M;Horiguchi, H;Kikuchi, S;Iyama, S;Ikeda, H;Goto, A;Kawano, Y;Murase, K;Takada, K;Miyanishi, K;Kato, J;Kobune, M; | miR-7977 inhibits the Hippo-YAP signaling pathway in bone marrow mesenchymal stromal cells | PloS one | 30835743 | r127; ab76252) and anti-NF2/Merlin Ab (ab88957) were purchased from Abcam (Tokyo, Japan). Human BM CD34+ cells and three different lot numbers of MSCs from healthy volunteers (HV) were purchased from AllCells, LLC (Toronto, Canada) (HV-derived MSCs # | Full Paper |
2019 | Chung, JE;Magis, W;Vu, J;Heo, SJ;Wartiovaara, K;Walters, MC;Kurita, R;Nakamura, Y;Boffelli, D;Martin, DIK;Corn, JE;DeWitt, MA; | CRISPR-Cas9 interrogation of a putative fetal globin repressor in human erythroid cells | PloS one | 30645582 | based on mobility using a separate FASC hemoglobin standard (Trinity biotech). Electroporation of CD34+ HSPCs with Cas9 RNPs HSPC electroporation was as described [28]. CD34+ HSPCs (G-CSF mobilized, Allcells, Inc.) were thawed and cultured in SFEM ( | Full Paper |
2019 | Hu, X;Shen, G;Hu, T;Guan, G;Wang, L; | Use of an in vitro dynamic culture system to assess flow shear forces upon cell adhesion within different structures | Journal of Chemical Technology & Biotechnology | Human umbilical vein endothelial cells (HUVEC001), provided by Allcells Company (Shanghai, China), were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, South American) and 1% penicillin-streptomycin (Gib | Full Paper | |
2019 | Satani, N;Giridhar, K;Cai, C;Wewior, N;Norris, DD;Olson, SD;Aronowski, J;Savitz, SI; | Aspirin in stroke patients modifies the immunomodulatory interactions of marrow stromal cells and monocytes | Brain research | 31220426 | MSCs from fresh human bone marrow aspirates (AllCells, Alameda, CA) were prepared from 3 separate healthy volunteers using density centrifugation and plastic adherence as previously described (Diaz et al., 2017, Kota et al., 2017, Parsha et al., 2017 | Full Paper |
2019 | Mohd Nor, NH;Berahim, Z;Azlina, A;Kannan, TP; | Identification of novel fibroblast-like cells from stem cells from human exfoliated deciduous teeth | Clinical oral investigations | 30847574 | Objectives This study aimed to differentiate and characterize fibroblast-like cells from stem cells from human exfoliated deciduous teeth (SHED). Materials and methods The Save | Full Paper |
2019 | McIntyre, PW;Wu, JL;Kolte, R;Zhang, R;Gregory, RL;Bruzzaniti, A;Yassen, GH; | The antimicrobial properties, cytotoxicity, and differentiation potential of double antibiotic intracanal medicaments loaded into hydrogel system | Clinical oral investigations | 29943369 | Human dental pulp stem cells (DPSC) (AllCells, Alameda, CA, USA) were cultured in 24-well plates (Cat: TP9024, Alkali Scientific Inc.) at concentrations of 3 × 104 cells/well in α-MEM (HyClone Laboratories Inc., South Logan, UT, USA) with 10% heat-in | Full Paper |
2019 | Zhong, X;Gao, W;Wu, R;Liu, H;Ge, J; | Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism | Molecular medicine reports | 31638185 | HUVECs were obtained from AllCells, LLC and cultured in ECM (Scien Cell Research Laboratories, Inc.). The 8-10th generations of HUVECs were used in this study and they were harvested for mRNA or protein detection after treatment with exosomes for 6 o | Full Paper |
2019 | Chen, T;Li, T;Wang, J; | p53 mediates PEDF‑induced autophagy in human umbilical vein endothelial cells through sestrin2 signaling | Molecular medicine reports | 31173218 | ed to investigate the association between the PEDF-p53-sestrin pathway and autophagy in human umbilical vein endothelial cells (HUVECs). Materials and methods Cell culture HUVECs were purchased from AllCells (Alameda, CA, USA). Cells were cultured i | Full Paper |
2019 | Feitosa, SA;Palasuk, J;Geraldeli, S;Windsor, LJ;Bottino, MC; | Physicochemical and biological properties of novel chlorhexidine-loaded nanotube-modified dentin adhesive | Journal of biomedical materials research. Part B, Applied biomaterials | 30199597 | The cytotoxicity of the CHX-loaded nanotube-modified adhesive and control groups (SBMP and HNT) were evaluated using human dental pulp stem cells (hDPSC, AllCells, Alameda, Save | Full Paper |
2019 | Kim, MM;Schlussel, L;Zhao, L;Himburg, HA; | Dickkopf-1 Treatment Stimulates Hematopoietic Regenerative Function in Infused Endothelial Progenitor Cells | Radiation research | 31081743 | Human bone marrow CD34+ cells (AllCells®, Alameda, CA) were irradiated at 3 Gy and added in noncontact to the upper portion of the transwell at a dose of 10,000 cells/well. Cells were cultured for 7 days, viable cells were enumerated by trypan blue e | Full Paper |
2019 | Choi, HW;Chan, C;Shterev, ID;Lynch, HE;Robinette, TJ;Johnson-Weaver, BT;Shi, J;Sempowski, GD;Kim, SY;Dickson, JK;Gooden, DM;Abraham, SN;Staats, HF; | Identification of Novel Mast Cell Activators Using Cell-Based High-Throughput Screening | SLAS discovery : advancing life sciences R & D | 30917061 | Primary human mast cells were differentiated from human CD34+ hematopoietic stem cells.19 Human CD34+ hematopoietic stem cells were obtained from commercial sources (Allcells). Isolated CD34+ cells was suspended at 5 × 105 cells/mL in IMDM medium sup | Full Paper |
2019 | Patel, A;Clementelli, CM;Jarocha, D;Mosoyan, G;Else, C;Kintali, M;Fong, H;Tong, J;Gordon, R;Gillespie, V;Keyzner, A;Poncz, M;Hoffman, R;Iancu-Rubin, C; | Pre-clinical development of a cryopreservable megakaryocytic cell product capable of sustained platelet production in mice | Transfusion | 31802511 | Division of Hematology and Medical Oncology, Tisch Cancer Institute and the Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York.++Division of Hematology and Medical Oncology, Tisch Cancer Institute and the Department o | Full Paper |
2019 | Md Hashim, S;Yusof, M;Zahari, W;Chandra, H;Ahmad Amin Noordin, K;Kannan, T;Sheikh Abdul Hamid, S;Mokhtar, K;Azlina, A; | Human Amniotic Membrane as a Matrix for Endothelial Differentiation of VEGF-Treated Dental Stem Cells | Cellular and Molecular Bioengineering | SHED (AllCells, USA) were cultured in the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, USA) supplemented with 15% of fetal bovine serum (FBS) (Gibco, USA) and 50 U/mL of penicillin-streptomycin (Gibco, USA). The cells were incubated in | Full Paper | |
2019 | Juillerat, A;Tkach, D;Busser, BW;Temburni, S;Valton, J;Duclert, A;Poirot, L;Depil, S;Duchateau, P; | Modulation of chimeric antigen receptor surface expression by a small molecule switch | BMC biotechnology | 31269942 | witching-off CAR T-cells. Overall, this could mitigate some of the toxicities that could occur with early intense antitumor responses. Methods T-cell proliferation Cryopreserved human PBMCs/T-cells (ALLCELLS, cat # PB006F) were used in accordance wi | Full Paper |
2019 | Kim, OH;Yoon, OJ;Lee, HJ; | Silk fibroin scaffolds potentiate immunomodulatory function of human mesenchymal stromal cells | Biochemical and biophysical research communications | 31506179 | Although mesenchymal stromal cells (MSCs) show great potential for use in regenerative medicine, their therapeutic efficacy remains limited because of their low adaptation efficiency Save | Full Paper |
2019 | Luo, H;Hu, L;Ma, B;Zhao, M;Luo, M;Deng, Q;Deng, S;Ye, H;Lin, T;Chen, J;Wang, T;Zhu, J;Lu, H; | Molecular dynamics based improvement of the solubilizing self-cleavable tag Zbasic-ΔI-CM application in the preparation of recombinant proteins in Escherichia coli | Biochemical and biophysical research communications | 30967267 | HUVEC was obtained from ATCC (Cat# CRL-1730) and cultured in ECM medium supplemented with 5% fetal bovine serum and 1 × ECGS (Allcells; Shanghai, China). For proliferation detection, the cells were harvested and re-suspended using the assay medium (E | Full Paper |
2019 | Camarillo, JM;Swaminathan, S;Abshiru, NA;Sikora, JW;Thomas, PM;Kelleher, NL; | Coupling Fluorescence-Activated Cell Sorting and Targeted Analysis of Histone Modification Profiles in Primary Human Leukocytes | Journal of the American Society for Mass Spectrometry | 31286445 | De-identified donor peripheral blood (PB) specimen were obtained from AllCells (Alameda, CA), Innovative Research (Novi, MI), or as clinical discards from the Clinical Flow Cytometry Laboratory at Northwestern Memorial Hospital. All procedures perfor | Full Paper |
2019 | Ishikawa, G;Fujiwara, N;Hirschfield, H;Varricchio, L;Hoshida, Y;Barosi, G;Rosti, V;Padilla, M;Mazzarini, M;Friedman, SL;Hoffman, R;Migliaccio, AR; | Shared and Tissue-Specific Expression Signatures between Bone Marrow from Primary Myelofibrosis and Essential Thrombocythemia | Experimental hematology | 31678370 | Bone marrow from eight healthy individuals was purchased from AllCells Technology (Oakland, CA). mRNA in amounts sufficient for analyses was obtained from 6 PMF and all ET Save | Full Paper |
2019 | Will, B;Kao, Y;Chen, J;Aivalioti, M;Tatiparthy, M; | 1029 - IRON HOMEOSTASIS-REGULATORY PATHWAYS IN HEMATOPOIETIC STEM CELLS | Experimental Hematology | Healthy cryopreserved human bone marrow cells and CD34+ enriched cells were purchased from AllCells (Alameda, CA) and ReachBio Research Labs (ReachBio LLC, WA). Fresh unprocessed bone marrow cells were purchased from Lonza (Lonza, MD), and the cells | Full Paper | |
2019 | Yang, H;Sun, J;Chen, H;Wang, F;Li, Y;Wang, H;Qu, T; | Mesenchymal stem cells from bone marrow attenuated the chronic morphine-induced cAMP accumulation in vitro | Neuroscience letters | 30605704 | Human bone marrow (n = 3) was purchased from AllCells Inc (Emeryville, CA). MSCs were isolated from the bone marrow according to previously described methods [16]. In brief, the bone marrow was diluted 1:1 with DMEM (low glucose, Invitrogen, CA, USA) | Full Paper |
2019 | Muruganandham, A;Dumont, C;Xing, K; | Approaching stability challenges for flow cytometry in a regulated bioanalytical environment | Bioanalysis | 31637937 | Mobilized human whole blood, with K 3 EDTA as anticoagulant, was purchased from ALLCELLS . Celerion stabilizer was added at 20% volume of blood within 24 h of collection and Save | Full Paper |
2019 | Choi, SA;An, JH;Lee, SH;Lee, GH;Yang, HJ;Jeong, PS;Cha, JJ;Lee, S;Park, YH;Song, BS;Sim, BW;Kim, YH;Kim, JS;Jin, YB;Huh, JW;Lee, SR;Lee, JH;Kim, SU; | Comparative Evaluation of Hormones and Hormone-Like Molecule in Lineage Specification of Human Induced Pluripotent Stem Cells | International journal of stem cells | 31242719 | mising other lineage decisions. Materials and Methods Cell culture, hiPSC generation, and lineage specification To obtain hiPSCs, CD34+ cells were purified from primary human bone marrow (BM) cells (Allcells, Emeryville, CA, USA) via immunomagnetic | Full Paper |
2019 | Kuramitsu, M;Okuma, K;Tezuka, K;Nakamura, H;Sagara, Y;Kurane, I;Hamaguchi, I; | Development and evaluation of human T-cell leukemia virus-1 and -2 multiplex quantitative PCR | Microbiology and immunology | 31429972 | Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells Inc. (Alameda, CA), TL-Om1 cells have been reported previously 15 and the MoT cell line (CRL-8066) was purchased from ATCC (Manassas, VA). Plasmid DNA pX1MT-M,16 an HTLV-1 molecu | Full Paper |
2019 | Li, X;Li, X;Lin, J;Sun, X;Ding, Q; | Exosomes Derived From Low-Intensity Pulsed Ultrasound-Treated Dendritic Cells Suppress Tumor Necrosis Factor-Induced Endothelial Inflammation | Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine | 30561085 | Human Umbilical Vein Endothelial Cell Culture Human umbilical vein endothelial cells were purchased from AllCells and cultured in endothelial cell medium (ScienCell, Shanghai, China) in a humidified atmosphere of 95% air and 5% CO2 at 37°C | Full Paper |
2019 | Choi, JH;Necsoiu, C;Wendorff, D;Jordan, B;Dixon, A;Roberts, TR;Beely, BM;Cancio, LC;Batchinsky, AI; | Effects of adjunct treatments on end-organ damage and histological injury severity in acute respiratory distress syndrome and multiorgan failure caused by smoke inhalation injury and burns | Burns : journal of the International Society for Burn Injuries | 31378621 | The process of disqualifying AssureImmune and qualifying AllCells as a new source of bone marrow for the production of MSCs delayed our timeline. 2. Based on our published Phase Save | Full Paper |
2019 | Ma, J;Lai, G;Lu, Z; | Effect of 410 nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor (VEGF) in cultured human vascular endothelial cells | Lasers in medical science | 30350123 | dilution with RPMI-1640 medium (ALLCELLS). Working drug solutions (0-8 μg/ml) were prepared by diluting the stock solution with RPMI-1640 medium (ALLCELLS. CA, USA). A light- Save | Full Paper |
2019 | Miura, S;Kobune, M;Horiguchi, H;Kikuchi, S;Iyama, S;Murase, K;Goto, A;Ikeda, H;Takada, K;Miyanishi, K;Kato, J; | EPO-R+ myelodysplastic cells with ring sideroblasts produce high erythroferrone levels to reduce hepcidin expression in hepatic cells | Blood cells, molecules & diseases | 31082798 | Total RNA derived from human BM CD34+, CB CD34+ and human mesenchymal stromal (MSCs) cells was purchased from AllCells, LLC (Toronto, Canada). Human myeloid leukemia Save | Full Paper |
2019 | Li, Y;Zhao, R;Li, X;Wang, C;Bao, H;Wang, S;Fang, J;Huang, J;Wang, C; | Blood-compatible Polyaniline Coated Electrospun Polyurethane Fiber Scaffolds for Enhanced Adhesion and Proliferation of Human Umbilical Vein Endothelial Cells | Fibers and Polymers | prothrombin time agent were purchased from AllCells Biological Technology Co., Ltd. Phosphate buffered saline (PBS) was purchased from Beijing Dingguo Changsheng Save | Full Paper | |
2019 | Yamasaki, AE;King, NE;Matsui, H;Jepsen, K;Panopoulos, AD; | Two iPSC lines generated from the bone marrow of a relapsed/refractory AML patient display normal karyotypes and myeloid differentiation potential | Stem cell research | 31739201 | 7q and 13q, were obtained from AllCells, LLC. Immunohistochemistry analysis of the patient's a normal karyotype (both analyses provided by AllCells, LLC). Previously published iPSCs Save | Full Paper |
2019 | CECHIM, G;CHIES, J; | In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review | Anais da Academia Brasileira de Ciências | Commercial leukopaks generally contain 80-200 mL of processed material with approximately 7x109peripheral blood mononuclear cells (PBMC), (more information available on: www.allcells.com/products/whole-tissue/leuko-pak). The Table I shows the percent | Full Paper | |
2019 | Coronado, RE;Somaraki-Cormier, M;Ong, JL;Halff, GA; | Hepatocyte-like cells derived from human amniotic epithelial, bone marrow, and adipose stromal cells display enhanced functionality when cultured on decellularized liver substrate | Stem cell research | 31163390 | Bone Marrow Derived Mesenchymal Stromal/Stem Cells: Bone marrow derived mesenchymal stem cells (BM-MSCs) were isolated from whole bone marrow aspirates (AllCells Alameda Save | Full Paper |
2019 | Abhyankar, VP;Bates, AM;Fischer, CL;Johnson, GK;Guthmiller, JM;Progulske-Fox, A;Brogden, KA; | Dataset on the chemokine and cytokine responses of multi-cell cultures treated with Porphyromonas gingivalis hemagglutinin B | Data in brief | 30740479 | HagB (QCL-1000, Chromogenic Limulus Amebocyte Lysate Assay, Lonza, Inc. Walkersville, MD USA). 2.2 Dendritic cells, GE keratinocytes, and T cells Non-peripheral blood human myeloid dendritic cells (AllCells, LLC, Alameda, CA USA) were thawed and sus | Full Paper |
2019 | Hii, SC;Luddin, N;Kannan, TP;Ab Rahman, I;Nik Abdul Ghani, NR; | The Biological Evaluation of Conventional and Nano-Hydroxyapatite-Silica Glass Ionomer Cement on Dental Pulp Stem Cells: A Comparative Study | Contemporary clinical dentistry | 32308298 | DPSCs purchased from AllCells, USA were used in this study. DPSCs were cultured in Alpha Minimum Essential Medium (α-MEM) (Gibco, Life Technologies, USA) supplemented with Save | Full Paper |
2019 | Akers, S; | Modeling central nervous system involvement in acute lymphoblastic leukemia | West Virginia University Libraries | Scientific Corporation, Westbury, NY) according to the manufacturer’s instructions. Positively selected peripheral blood CD19+ B-cells from a healthy donor were purchased from AllCells (Emeryville, CA). Human brain-derived microvascular endothelial c | Full Paper | |
2019 | Barve, A; | Characterization of a more clinically relevant human leukemia xenograft model to examine perturbation of MET/SAM metabolism as a novel therapeutic paradigm for MLL-R leukemia in vivo. | University of Louisville | Acute myeloid leukemia (AML), is a heterogeneous clonal disorder characterized by an accumulation of malignant myeloid progenitors in the bone marrow (BM), hindering normal Save | Full Paper | |
2019 | Liu, H;Kezios, S; | DYNAMIC CHARACTERISTICS OF ETTINGSHAUSEN REFRIGERATOR | Proceeding of International Heat Transfer Conference 4 | However this was not the case in allcells and in cells from plant 41, with 3 accessory chromo¬somes, sometimes none, one, two or all three accessorychromosomes divided precociously Save | Full Paper | |
2019 | Rodriguez, L;Mohammadipoor, A;Kamucheka, R;Asher, A;Alvarado, L;Antebi, B;Cancio, L; | Preconditioning in an Acute Respiratory Distress Syndrome-Like Milieu Augments the Immunomodulatory and Anti-Inflammatory Function of MSCS | A60. LUNG INJURY, SEPSIS, AND ARDS | Methods: Human bone marrow (BM)-MSCs were isolated from fresh mononuclear cells purchased from AllCells (Alameda, CA). Human adipose-derived (AD)-MSCs were isolated from Save | Full Paper | |
2019 | De Maio, V;Brandic, I; | Multi-Objective Mobile Edge Provisioning in Small Cell Clouds | Proceedings of the 2019 ACM/SPEC International Conference on Performance Engineering | Also, we can observe significant losses when using the other two algorithms, ALLCELLS and RANDOM. In the first case this is because the algorithm overestimates the number of Edge Save | Full Paper | |
2019 | Lal, A;Chiang, Z;Yakovenko, N;Duarte, F;Israeli, J;Buenrostro, J; | AtacWorks: A deep convolutional neural network toolkit for epigenomics | bioRxiv | Cryopreserved human bone marrow mononuclear cells were purchased from Allcells (catalog number BM, CR, MNC, 10M). Cells were quickly thawed in a 37°C water bath, rinsed with culture medium (RPMI 1640 medium supplemented with 15% FBS) and | Full Paper | |
2019 | Liu, J;Kozhaya, L;Torres, V;Unutmaz, D;Lu, M; | Structure-Based Discovery of a Novel Small-Molecule Inhibitor of Methicillin-Resistant S. aureus | bioRxiv | Blood samples were obtained from healthy, consenting donors as Buffy coats (New York Blood Center) and leukopaks (AllCells, Alameda, CA). PBMCs were isolated from peripheral blood by density gradient centrifugation using Ficoll-Paque | Full Paper | |
2019 | Wang, X;Derakhshandeh, R;Rodriguez, H;Han, D;Kostyushev, D;Henry, T;Traverse, J;Moyé, L;Simari, R;Taylor, D;Springer, M; | Impaired Therapeutic Efficacy of Bone Marrow Cells from Post-Myocardial Infarction Patients in the TIME and LateTIME Clinical Trials | bioRxiv | Healthy de-identified donor BM MNCs were purchased from AllCells (Alameda, CA). These MNCs were manually isolated at AllCells using Ficoll conditions like that in the Sepax Save | Full Paper | |
2019 | Granja, J;Klemm, S;McGinnis, L;Kathiria, A;Mezger, A;Parks, B;Gars, E;Liedtke, M;Zheng, G;Chang, H;Majeti, R;Greenleaf, W; | A single cell framework for multi-omic analysis of disease identifies malignant regulatory signatures in mixed phenotype acute leukemia | bioRxiv | PBMCs, BMMCs, and CD34+ bone marrow cells were obtained from healthy donors (AllCells). Description of Leukemic Patients/Donors Patient samples were collected with informed consent prospectively under a protocol approved by the Institutional Review | Full Paper | |
2019 | Ding, J;Adiconis, X;Simmons, S;Kowalczyk, M;Hession, C;Marjanovic, N;Hughes, T;Wadsworth, M;Burks, T;Nguyen, L;Kwon, J;Barak, B;Ge, W;Kedaigle, A;Carroll, S;Li, S;Hacohen, N;Rozenblatt-Rosen, O;Shalek, A;Villani, A;Regev, A;Levin, J; | Systematic comparative analysis of single cell RNA-sequencing methods | bioRxiv | We acquired commercially-available PBMCs (AllCells; Lot #PB006F-A5865) as frozen aliquots with 25 million cells for the PBMC1 experiment or isolated them from fresh blood (AllCells Save | Full Paper | |
2019 | Fan, L;Zhao, M; | Gold nanorod based delivery system could bring about superior therapy effect of Ramucirumab through direct cytotoxicity to cancer cell mediated by differential regulation of phagocytosis in gastric cancer cell | bioRxiv | HUVEC cell was purchased from Allcells (Endothelial Cell Medium, H-004B, Allcells, supplemented with 5%fetal bovine serum), GC cells SNU-5 (1640 Cell Medium, Gibco, supplemented with 10% fetal bovine serum) cell was purchased from ATCC and MKN | Full Paper | |
2019 | King, GR; | Comparative systemic analysis of human immunoglobulin repertoires | Thesis | All peripheral blood and bone marrow aspirate samples were collected by AllCells and shipped overnight at ambient temperature; processing was started immediately upon receipt of the samples in a sterilized biological safety hood. Sterile 1x PBS pH 7. | Full Paper | |
2019 | Sakhnevych, S; | Tim-3-galectin-9 immunosuppressive pathway in human acute myeloid leukaemia and solid tumour cells and biochemical functions of its crucial components | Thesis | Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordance with the manufacturer's instructions following ethical approval (REC reference: 16-SS-033). Other p | Full Paper | |
2019 | Lee, MJ; | The Development of Novel Proteasome Inhibitors for the Treatment of Multiple Myeloma and Alzheimer's Disease | Thesis | Over a decade, proteasome inhibitors (PIs), bortezomib, carfilzomib (Cfz) and ixazomib, have contributed to a significant improvement in the overall survival for multiple myeloma (MM) Save | Full Paper | |
2019 | Kuhlman, BM; | MICROGRAVITY NEGATIVELY AFFECTS DNA DAMAGE REPAIR AND THE ANTI-LEUKEMIC FUNCTION OF HUMAN NATURAL KILLER CELLS | Thesis | Healthy human bone marrow (BM) mononuclear cells (BMMNC) were purchased from AllCells, LLC (Alameda, CA, USA). CD34+ HSC were isolated from BMMNC using antihuman CD34 MicroBeads and the MiniMACS magnetic-based positive-selection system (Miltenyi Biot | Full Paper | |
2019 | 龚子祯, ;蔡海波, ;谭文松, ; | 黄原胶支持 CIK 细胞高密度扩增 | 高校化学工程学报 | RPMI 1640购于美国Gibco公司; 血清培养基(自配);PBMC购于上海Allcells公司;CD3单克隆 抗体购于美国eBioscience公司;植物血凝 PHA购于美国Sigma-Aldrich公司;干扰 (IFN-γ),白介 Save | Full Paper | |
2019 | Cao, YP;Pan, M;Song, YL;Zhang, HL; | MiR-302 a/b/c suppresses tumor angiogenesis in hepatocellular carcinoma by targeting MACC1 | European Review for Medical and Pharmacological Sciences | The umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Huh7 and PLC/PRF/5 cells were incubated with Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine ser | Full Paper | |
2019 | 王一唯, ;黄婷, ;孙笑, ;王玉东, ; | RANKL 募集调节性 T 细胞促进子宫内膜癌侵袭的研究 | 中国免疫学杂志 | 科院细胞库),外周单个 细胞(美国ALLCELLS 公 司),RANK 慢病毒(上海和元生物科技公司),重组 人TGF鄄茁,重组人RANKL(美国Peprotech 公司),人 RANKL ELISA 试剂盒(武汉博士德生物 Save | Full Paper | |
2019 | Alvarado, L; | Evaluating the Therapeutic Effects of Mesenchymal Stem Cells in an in Vitro Co-Culture System of a Damaged Alveolar Barrier | Thesis | Human BM-MSCs were isolated from commercially available MNCs (AllCells LLC; Emeryville, CA). Bone marrow specimen were obtained from human donors 20-30 years of age. MNCs were cultured on standard culture flasks at a density of 3 x 105 cells/cm2 in | Full Paper | |
2019 | Romero-Masters, J; | Studying Epstein-Barr virus-induced lymphomagenesis in a cord-blood humanized mouse model | Thesis | Creation of cord blood-humanized NOD/LtSz-scid/IL2Rγnull mice: Immunodeficient NSG (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson labs (catalogue number: 005557). Commercially available CD34-depleted human cord blood mononuclear cells (Al | Full Paper | |
2019 | Mumbach, MR; | Technologies for Protein and RNA-Focused Chromosome Conformation | Thesis | Normal donor human peripheral blood cells were obtained fresh from AllCells. CD4+ T cells were enriched from peripheral blood using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). For CD4+ T helper cell subtypes, Naïve T c | Full Paper | |
2019 | Walker, KL; | Non-chemotherapeutic Methods of Treating T Cell Acute Lymphoblastic Leukemia | Thesis | Chapter 4.2: EOpansion of NK cell products Fresh leukopaks were commercially o9tained (AllCells, Alameda, CA) from paid human apheresis donors, stored at 1Г10o C, and Save | Full Paper | |
2019 | Srivastava, S;Salter, AI;Liggitt, D;Yechan-Gunja, S;Sarvothama, M;Cooper, K;Smythe, KS;Dudakov, JA;Pierce, RH;Rader, C;Riddell, SR; | Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting | Cancer cell | 30889382 | CFU Assays Femurs and tibia from control and ROR1 CAR-T cell-treated mice were harvested 9 days post-T cell transfer and submitted to ReachBio Inc. for CFU-GM, BFU-E, CFU-Mk, Pre-B CFC, and CFU-F assays | Full Paper |
2019 | Humbert, O;Radtke, S;Samuelson, C;Carrillo, RR;Perez, AM;Reddy, SS;Lux, C;Pattabhi, S;Schefter, LE;Negre, O;Lee, CM;Bao, G;Adair, JE;Peterson, CW;Rawlings, DJ;Scharenberg, AM;Kiem, HP; | Therapeutically relevant engraftment of a CRISPR-Cas9-edited HSC-enriched population with HbF reactivation in nonhuman primates | Science translational medicine | 31366580 | For CFC assays, 1000-1200 sorted cells were seeded into 3.5 ml ColonyGEL 1402 (ReachBio). Hematopoietic colonies were scored after 12-14 days. Arising colonies were identified as colony-forming unit- (CFU-) granulocyte (CFU-G), macrophage | Full Paper |
2019 | Crawford, A;Haber, L;Kelly, MP;Vazzana, K;Canova, L;Ram, P;Pawashe, A;Finney, J;Jalal, S;Chiu, D;Colleton, CA;Garnova, E;Makonnen, S;Hickey, C;Krueger, P;DelFino, F;Potocky, T;Kuhnert, J;Godin, S;Retter, MW;Duramad, P;MacDonald, D;Olson, WC;Fairhurst, J;Huang, T;Martin, J;Lin, JC;Smith, E;Thurston, G;Kirshner, JR; | A Mucin 16 bispecific T cell-engaging antibody for the treatment of ovarian cancer | Science translational medicine | 31217340 | Regeneron Pharmaceuticals Institutional Animal Care and Use Committee. NSG (20 to 24 weeks old) mice (the Jackson Laboratory) were injected with 5 × 106 human PBMCs (ReachBio) before 2 × 106 ascites cells from the OVCAR-3/Luc cell line, | Full Paper |
2019 | Li, C;Mishra, AS;Gil, S;Wang, M;Georgakopoulou, A;Papayannopoulou, T;Hawkins, RD;Lieber, A; | Targeted Integration and High-Level Transgene Expression in AAVS1 Transgenic Mice after In Vivo HSC Transduction with HDAd5/35++ Vectors | Molecular therapy : the journal of the American Society of Gene Therapy | 31494053 | 2,500 Lin− cells were plated in triplicates in ColonyGEL 1202 mouse complete medium (ReachBio, Seattle, WA) and incubated for 12 days at 37°C in 5% CO2 and maximum humidity. Colonies were enumerated using a Leica MS 5 dissection microscope (Leica Mic | Full Paper |
2019 | Kuhlmann, AS;Haworth, KG;Barber-Axthelm, IM;Ironside, C;Giese, MA;Peterson, CW;Kiem, HP; | Long-Term Persistence of Anti-HIV Broadly Neutralizing Antibody-Secreting Hematopoietic Cells in Humanized Mice | Molecular therapy : the journal of the American Society of Gene Therapy | 30391142 | erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor (GM-CSF). At necropsy, cells isolated from bone marrow (BM) were seeded in triplicate into ColonyGEL (ReachBio, Seattle WA). Hem | Full Paper |
2019 | Humbert, O;Laszlo, GS;Sichel, S;Ironside, C;Haworth, KG;Bates, OM;Beddoe, ME;Carrillo, RR;Kiem, HP;Walter, RB; | Engineering resistance to CD33-targeted immunotherapy in normal hematopoiesis by CRISPR/Cas9-deletion of CD33 exon 2 | Leukemia | 30291334 | system (Harvard Apparatus, Cambridge, MA) [12]. For evaluation of colony-forming units (CFUs), 1500 CD34+ cells were seeded in 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA) and scored after 12-14 days. CFU DNA was extracted in QuickExtract (Epicentre | Full Paper |
2019 | Ly, M;Rentas, S;Vujovic, A;Wong, N;Moreira, S;Xu, J;Holzapfel, N;Bhatia, S;Tran, D;Minden, MD;Draper, JS;Hope, KJ; | Diminished AHR Signaling Drives Human Acute Myeloid Leukemia Stem Cell Maintenance | Cancer research | 31519687 | Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio), supplemented with DMSO or 750 Save | Full Paper |
2019 | Smitheman, KN;Severson, TM;Rajapurkar, SR;McCabe, MT;Karpinich, N;Foley, J;Pappalardi, MB;Hughes, A;Halsey, W;Thomas, E;Traini, C;Federowicz, KE;Laraio, J;Mobegi, F;Ferron-Brady, G;Prinjha, RK;Carpenter, CL;Kruger, RG;Wessels, L;Mohammad, HP; | Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes | Haematologica | 30514804 | uated for each assay. Primary acute myeloid leukemia blast colony Clonogenic progenitors from primary AML patients’ samples were assessed in a semi-solid methylcellulose-based medium (ColonyGel , ReachBio) containing recombinant human (rh) SCF (50 ng | Full Paper |
2019 | Fuller, NO;Pirone, A;Lynch, BA;Hewitt, MC;Quinton, MS;McKee, TD;Ivarsson, M; | CoREST Complex-Selective Histone Deacetylase Inhibitors Show Prosynaptic Effects and an Improved Safety Profile To Enable Treatment of Synaptopathies | ACS chemical neuroscience | 30496686 | Normal human bone marrow light density cells derived from normal bone marrow (NorCal Biologics, California) and qualified at ReachBio were stored in the gaseous phase of liquid Save;minimum peptide length of at least 7 amino acids was required. Ef | Full Paper |
2019 | Decker, CE;Young, T;Pasnikowski, E;Chiu, J;Song, H;Wei, Y;Thurston, G;Daly, C; | Genome-scale CRISPR activation screen uncovers tumor-intrinsic modulators of CD3 bispecific antibody efficacy | Scientific reports | 31882897 | Healthy donor peripheral blood mononuclear cells (PBMCs) were purchased from ReachBio Research Labs. T cells were isolated by negative selection using the Dynabeads Save;Clec12axCD3 bsAbs were isolated by selective protein A affinity chromatography | Full Paper |
2019 | Ostrakhovitch, EA;Akakura, S;Sanokawa-Akakura, R;Tabibzadeh, S; | 3-Mercaptopyruvate sulfurtransferase disruption in dermal fibroblasts facilitates adipogenic trans-differentiation | Experimental cell research | 31634482 | Human dermal fibroblasts (HDF) were obtained from ATCC (Manassas, VA). Mesenchymal stem cells (MSCs), isolated from a 25-year-old male donor, were purchased from ReachBio Research lab (Seattle, WA). Chemicals were from Sigma-Aldrich Company (St Louis | Full Paper |
2019 | Radtke, S;Chan, YY;Sippel, TR;Kiem, HP;Rongvaux, A; | MISTRG mice support engraftment and assessment of nonhuman primate hematopoietic stem and progenitor cells | Experimental hematology | 30590092 | For CFC assays, 1000-1200 sort-purified NHP or CD34-subpopulations were seeded into 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA). Hematopoietic colonies were scored after 12-14 days. Arising colonies were identified as colony-forming unit- (CFU-) gr | Full Paper |
2019 | Radtke, S;Perez, AM;Venkataraman, R;Reddy, S;Haworth, KG;Humbert, O;Kiem, HP;Peterson, CW; | Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells | Journal of visualized experiments : JoVE | 30829324 | Protamine sulfate Sigma P-4505 14 mL Polypropylene Round-Bottom Tube Corning 352059 Colony Gel 1402 ReachBio 1402 QuadroMACS Separators Miltenyi Biotec 130-090-976 | Full Paper |
2018 | Cristescu, R;Mogg, R;Ayers, M;Albright, A;Murphy, E;Yearley, J;Sher, X;Liu, XQ;Lu, H;Nebozhyn, M;Zhang, C;Lunceford, JK;Joe, A;Cheng, J;Webber, AL;Ibrahim, N;Plimack, ER;Ott, PA;Seiwert, TY;Ribas, A;McClanahan, TK;Tomassini, JE;Loboda, A;Kaufman, D; | Pan-tumor genomic biomarkers for PD-1 checkpoint blockade-based immunotherapy | Science | 30309915 | Associations of TMB and the T cell–inflamed GEP with BOR and PFS were evaluated by using tumor samples from subgroups of patients treated with pembrolizumab in clinical trials who had WES data available. These included a discovery cohort of patients | Full Paper |
2018 | Smith, S;Lynch, R;Till, B;Cowan, A;Wu, Q;Voutsinas, J;Shadman, M;Blue, K;Shustov, A;Kanan, S;Low, L;Cassaday, R;Fromm, J;Gopal, A; | Pembrolizumab in Combination with Standard RCHOP Therapy for Previously Untreated Diffuse Large B-Cell Lymphoma | Blood | Secondary endpoints included response rates, overall (OS) and progression-free survival (PFS). Baseline PDL1 expression was analyzed centrally (Merck/Qualtek). Peripheral blood flow cytometry for changes in PD1, PDL-1, and PDL-2 subsets were performe | Full Paper | |
2018 | Doi, T;Piha-Paul, SA;Jalal, SI;Saraf, S;Lunceford, J;Koshiji, M;Bennouna, J; | Safety and Antitumor Activity of the Anti-Programmed Death-1 Antibody Pembrolizumab in Patients With Advanced Esophageal Carcinoma | J. Clin. Oncol. | 29116900 | PD-L1 Expression Analysis PD-L1 expression was assessed at screening by a central laboratory on the basis of a laboratory-developed prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA) using either an archived (formalin-fi | Full Paper |
2018 | Kanjanapan, Y;Lien, S;Yang, C;Clouthier, D;Elston, S;Jiang, H;Butler, M;Hogg, D;Ohashi, P;Pugh, T;Siu, L;Spreafico, A; | Genomic and immune landscape of metastatic melanoma (MM) treated with pembrolizumab (PEM). | JCO | Baseline (BL) and on treatment (post 2-3 doses of PEM) fresh tumor biopsies were collected for DNA/RNA sequencing, immune-profiling, and tumor PD-L1 expression by immunohistochemistry (QualTek), with longitudinal blood collection for immunophenotypi | Full Paper | |
2018 | Graff, J;Alumkal, J;Thompson, R;Moran, A;Thomas, G;Wood, M;Drake, C;Slottke, R;Beer, T; | Pembrolizumab (Pembro) plus enzalutamide (Enz) in metastatic castration resistant prostate cancer (mCRPC): Extended follow up. | JCO | The other 2 had neither. Of the NR who had sequencing, 4 had a DNA repair defects, and none had MSI. None of the biopsies showed tumoral PD-L1 expression (Qualtek, 22C3). Conclusions: Pembro has activity in mCRPC when added to Enz | Full Paper | |
2018 | Kelly, C;Bowler, T;Munhoz, R;Chi, P;Dickson, M;Gounder, M;Keohan, M;Dholakia, R;Condy, M;Singer, S;Crago, A;Yoon, S;Ariyan, C;Hwang, S;Erinjeri, J;Antonescu, C;Qin, L;Tap, W;D'Angelo, S; | A phase II study of talimogene laherparepvec (T-VEC) and pembrolizumab in patients with metastatic sarcoma. | JCO | overall survival. Correlative studies included PD-L1 expression by IHC (Qualtek), multiplex IHC, characterization of tumor infiltrating lymphocytes, mutational burden/neoantigen analysis, and T cell receptor clonality. Results | Full Paper | |
2018 | Smith, JJ;Timoshevskaya, N;Ye, C;Holt, C;Keinath, MC;Parker, HJ;Cook, ME;Hess, JE;Narum, SR;Lamanna, F;Kaessmann, H;Timoshevskiy, VA;Waterbury, CKM;Saraceno, C;Wiedemann, LM;Robb, SMC;Baker, C;Eichler, EE;Hockman, D;Sauka-Spengler, T;Yandell, M;Krumlauf, R;Elgar, G;Amemiya, CT; | The sea lamprey germline genome provides insights into programmed genome rearrangement and vertebrate evolution | Nat. Genet. | 29358652 | Sequencing. Fragment libraries were prepared by Covaris shearing of sperm genomic DNA isolated from a single individual and size selected to achieve average insert sizes of ~205 and 231bp. These libraries were sequenced on the Illumina HiSeq2000 plat | Full Paper |
2018 | D'Angelo, SP;Melchiori, L;Merchant, MS;Bernstein, D;Glod, J;Kaplan, R;Grupp, S;Tap, WD;Chagin, K;Binder, GK;Basu, S;Lowther, DE;Wang, R;Bath, N;Tipping, A;Betts, G;Ramachandran, I;Navenot, JM;Zhang, H;Wells, DK;Van Winkle, E;Kari, G;Trivedi, T;Holdich, T;Pandite, L;Amado, R;Mackall, CL; | Antitumor Activity Associated with Prolonged Persistence of Adoptively Transferred NY-ESO-1 c259T Cells in Synovial Sarcoma | Cancer Discov | 29891538 | Serial 5-μm sections of formalin-fixed, paraffin-embedded tissues were analyzed for the following single markers by IHC: NY-ESO-1, Pan-CK (Clone AE1/AE3/PCK26, Ventana), CD45 (Clone 2B11 + PD7/26, Ventana), CD3 (Clone 2GV6, Ventana), CD8 (Clone C8/14 | Full Paper |
2018 | Dejanovic, B;Huntley, MA;De Mazière, A;Meilandt, WJ;Wu, T;Srinivasan, K;Jiang, Z;Gandham, V;Friedman, BA;Ngu, H;Foreman, O;Carano, RAD;Chih, B;Klumperman, J;Bakalarski, C;Hanson, JE;Sheng, M; | Changes in the Synaptic Proteome in Tauopathy and Rescue of Tau-Induced Synapse Loss by C1q Antibodies | Neuron | 30392797 | Biological Samples Human brain tissue Folio Biosciences N/A | Full Paper |
2018 | Schafer, PH;Ye, Y;Wu, L;Kosek, J;Ringheim, G;Yang, Z;Liu, L;Thomas, M;Palmisano, M;Chopra, R; | Cereblon modulator iberdomide induces degradation of the transcription factors Ikaros and Aiolos: immunomodulation in healthy volunteers and relevance to systemic lupus erythematosus | Ann. Rheum. Dis. | 29945920 | study in healthy volunteers. Methods. Gene expression studies. Viably frozen PBMCs from 11 patients with SLE and 10 healthy volunteers were obtained from Conversant Bio (Huntsville, Alabama, USA). Informed consent was | Full Paper |
2018 | Liles, JT;Corkey, BK;Notte, GT;Budas, GR;Lansdon, EB;Hinojosa-Kirschenbaum, F;Badal, SS;Lee, M;Schultz, BE;Wise, S;Pendem, S;Graupe, M;Castonguay, L;Koch, KA;Wong, MH;Papalia, GA;French, DM;Sullivan, T;Huntzicker, EG;Ma, FY;Nikolic-Paterson, DJ;Altuhaifi, T;Yang, H;Fogo, AB;Breckenridge, DG; | ASK1 contributes to fibrosis and dysfunction in models of kidney disease | J. Clin. Invest. | 30024858 | ... NAL BIOPSIES AND DB/DB ENOS–/– MOUSE KIDNEYS Formalin-fixed paraffin-embedded renal biopsies from 10 patients with DKD and from 7 donors without kidney disease (“healthy”) were obtained from Folio Biosciences. Kidney sections were prepared from | Full Paper |
2018 | Gadgeel, SM;Pennell, NA;Fidler, MJ;Halmos, B;Bonomi, P;Stevenson, J;Schneider, B;Sukari, A;Ventimiglia, J;Chen, W;Galasso, C;Wozniak, A;Boerner, J;Kalemkerian, GP; | Phase II Study of Maintenance Pembrolizumab in Patients with Extensive-Stage Small Cell Lung Cancer (SCLC) | J Thorac Oncol | 29775808 | than every four cycles. Retrieval of pretreatment biopsy specimens for assessment of PD-L1 expression in tumor cells and stromal tissue was conducted by Qualtek Clinical Laboratories (Newton, PA). Assessment of tumor PD | Full Paper |
2018 | Roberts, BS;Hardigan, AA;Moore, DE;Ramaker, RC;Jones, AL;Fitz-Gerald, MB;Cooper, GM;Wilcox, CM;Kimberly, RP;Myers, RM; | Discovery and Validation of Circulating Biomarkers of Colorectal Adenoma by High-Depth Small RNA Sequencing | Clin. Cancer Res. | 29490987 | HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama. | Full Paper |
2018 | Tucker, DW;Getchell, CR;McCarthy, ET;Ohman, AW;Sasamoto, N;Xu, S;Ko, JY;Gupta, M;Shafrir, A;Medina, JE;Lee, JJ;MacDonald, LA;Malik, A;Hasselblatt, KT;Li, W;Zhang, H;Kaplan, SJ;Murphy, GF;Hirsch, MS;Liu, JF;Matulonis, UA;Terry, KL;Lian, CG;Dinulescu, DM; | Epigenetic Reprogramming Strategies to Reverse Global Loss of 5-Hydroxymethylcytosine, a Prognostic Factor for Poor Survival in High-grade Serous Ovarian Cancer | Clin. Cancer Res. | 29263182 | ... s of 5-hmC levels in a large biorepository of HGSOC cases by using a patient tumor microarray (TMA) from the Dana-Farber Cancer Institute (DFCI, Boston, MA; ref. 22) and a commercially available TMA (Folio Biosciences). We observed strong 5-hmC s | Full Paper |
2018 | Subbiah, V;Murthy, R;Hong, DS;Prins, RM;Hosing, C;Hendricks, K;Kolli, D;Noffsinger, L;Brown, R;McGuire, M;Fu, S;Piha-Paul, S;Naing, A;Conley, AP;Benjamin, RS;Kaur, I;Bosch, ML; | Cytokines Produced by Dendritic Cells Administered Intratumorally Correlate with Clinical Outcome in Patients with Diverse Cancers | Clin. Cancer Res. | 30018119 | Biopsied tumors were formalin fixed and paraffin embedded (FFPE) using standard methods. All immunohistochemistry was performed by QualTek Molecular Laboratories (Santa Barbara, CA). In situ detection of IFNγ and TNFα transcripts in FFPE specimens w | Full Paper |
2018 | Joseph, RW;Elassaiss-Schaap, J;Kefford, R;Hwu, WJ;Wolchok, JD;Joshua, AM;Ribas, A;Hodi, FS;Hamid, O;Robert, C;Daud, A;Dronca, R;Hersey, P;Weber, JS;Patnaik, A;de Alwis, DP;Perrone, A;Zhang, J;Kang, SP;Ebbinghaus, S;Anderson, KM;Gangadhar, TC; | Baseline Tumor Size Is an Independent Prognostic Factor for Overall Survival in Patients with Melanoma Treated with Pembrolizumab | Clin. Cancer Res. | 29685882 | Tumor PD-L1 expression was assessed by a prototype IHC assay (QualTek Molecular Laboratories; ref. 14) in pretreatment tumor biopsy samples using the 22C3 antibody (Merck & Co., Inc.). PD-L1 positivity was defined as membranous staining in 1% of tumo | Full Paper |
2018 | Rugo, HS;Delord, JP;Im, SA;Ott, PA;Piha-Paul, SA;Bedard, PL;Sachdev, J;Le Tourneau, C;van Brummelen, EMJ;Varga, A;Salgado, R;Loi, S;Saraf, S;Pietrangelo, D;Karantza, V;Tan, AR; | Safety and Antitumor Activity of Pembrolizumab in Patients with Estrogen Receptor-Positive/Human Epidermal Growth Factor Receptor 2-Negative Advanced Breast Cancer | Clin. Cancer Res. | 29559561 | An archival or newly obtained core or excisional biopsy specimen from a nonirradiated tumor lesion was collected from each patient for assessment of PD-L1 expression. Tumor PD-L1 expression was assessed by immunohistochemistry at a central laboratory | Full Paper |
2018 | Thompson, ML;Finnila, CR;Bowling, KM;Brothers, KB;Neu, MB;Amaral, MD;Hiatt, SM;East, KM;Gray, DE;Lawlor, JMJ;Kelley, WV;Lose, EJ;Rich, CA;Simmons, S;Levy, SE;Myers, RM;Barsh, GS;Bebin, EM;Cooper, GM; | Genomic sequencing identifies secondary findings in a cohort of parent study participants | Genet. Med. | 29790872 | to change their preferences at the return of results appointment. Whole exome and genome sequencing Blood samples were sent for sequencing at the HudsonAlpha Genomic Services Laboratory (http://gsl.hudsonalpha.org). Genomic DNA was isolated from peri | Full Paper |
2018 | Pena, LDM;Jiang, YH;Schoch, K;Spillmann, RC;Walley, N;Stong, N;Rapisardo Horn, S;Sullivan, JA;McConkie-Rosell, A;Kansagra, S;Smith, EC;El-Dairi, M;Bellet, J;Keels, MA;Jasien, J;Kranz, PG;Noel, R;Nagaraj, SK;Lark, RK;Wechsler, DSG;Del Gaudio, D;Leung, ML;Hendon, LG;Parker, CC;Jones, KL;, ;Goldstein, DB;Shashi, V; | Looking beyond the exome: a phenotype-first approach to molecular diagnostic resolution in rare and undiagnosed diseases | Genet. Med. | 28914269 | Kimberly Splinter Harvard University (CC) David P. Bick HudsonAlpha Camille L. Birch HudsonAlpha Braden E. Boone HudsonAlpha Donna M. Brown HudsonAlpha Daniel C. Dorset HudsonAlpha Lori H. Handley HudsonAlpha Howard J. Jacob HudsonAlpha Angela L. Jo | Full Paper |
2018 | Abraham, B;Pharma, GUCB;Sankyo, HD; | Disclosure of relevant financial relationships | Journal of the American Academy of Dermatology | Abramovits, William; Grants - AbbVie, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant Bio, Dermavant Abramovits, William; Honoraria - AbbVie, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant Bio, D | Full Paper | |
2018 | Menard, LC;Fischer, P;Kakrecha, B;Linsley, PS;Wambre, E;Liu, MC;Rust, BJ;Lee, D;Penhallow, B;Manjarrez Orduno, N;Nadler, SG; | Renal Cell Carcinoma (RCC) Tumors Display Large Expansion of Double Positive (DP) CD4+CD8+ T Cells With Expression of Exhaustion Markers | Front Immunol | 30534127 | Samples for the discovery and replication cohorts were provided by Folio-Conversant Bio (AL), MT Group (CA), Rutgers-CINJ or the Benaroya Research Institute. All patients gave written informed consent at time of sample collection according to IRB pro | Full Paper |
2018 | Tisdale, J;Kanter, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Schmidt, M;Miller, A;Pierciey, F;Shi, W;Ribeil, J;Asmal, M;Thompson, A;Walters, M; | Current Results of Lentiglobin Gene Therapy in Patients with Severe Sickle Cell Disease Treated Under a Refined Protocol in the Phase 1 Hgb-206 Study | Blood | Background β-globin gene transfer has the potential for substantial clinical benefit in patients with sickle cell disease (SCD). LentiGlobin Drug Product (DP) contains autologous CD34+ hematopoietic stem cells (HSCs) transduced with th | Full Paper | |
2018 | Klemann, CJHM;Xicoy, H;Poelmans, G;Bloem, BR;Martens, GJM;Visser, JE; | Physical Exercise Modulates L-DOPA-Regulated Molecular Pathways in the MPTP Mouse Model of Parkinson's Disease | Mol. Neurobiol. | 29019056 | pooled for RNAseq analysis. RNA Sequencing and Data Processing. All RNA samples were subjected to RNA sequencing (RNAseq; HudsonAlpha Genomic Services Lab, Huntsville, AL) as performed before [37]. In short, total | Full Paper |
2018 | Van Laar, R;Lincoln, M;Van Laar, B; | Development and validation of a plasma-based melanoma biomarker suitable for clinical use | Br. J. Cancer | 29360813 | expression profiles of individuals with and without melanoma, 0.5-2 ml of cell-free plasma was obtained from 16 healthy controls and 32 Caucasian individuals at the time of their diagnosis with cutaneous melanoma (Cureline Inc., CA, USA and Folio Bio | Full Paper |
2018 | Buatois, V;Johnson, Z;Salgado-Pires, S;Papaioannou, A;Hatterer, E;Chauchet, X;Richard, F;Barba, L;Daubeuf, B;Cons, L;Broyer, L;D'Asaro, M;Matthes, T;LeGallou, S;Fest, T;Tarte, K;Clarke Hinojosa, RK;Genescà Ferrer, E;Ribera, JM;Dey, A;Bailey, K;Fielding, AK;Eissenberg, L;Ritchey, J;Rettig, M;DiPersio, JF;Kosco-Vilbois, MH;Masternak, K;Fischer, N;Shang, L;Ferlin, WG; | Preclinical Development of a Bispecific Antibody that Safely and Effectively Targets CD19 and CD47 for the Treatment of B-Cell Lymphoma and Leukemia | Mol. Cancer Ther. | 29743205 | Cells were cultured at 37°C and 5% CO2. Primary samples were collected from Conversant Biologics (Huntsville, USA), the Leukemia Research Center (Glasgow, UK), the Geneva University Hospital (Geneva, Switzerland) ;... tometry and finally selecte | Full Paper |
2018 | Foulk, B;Schaffer, M;Gross, S;Rao, C;Smirnov, D;Connelly, MC;Chaturvedi, S;Reddy, M;Brittingham, G;Mata, M;Repollet, M;Rojas, C;Auclair, D;DeRome, M;, ;Weiss, B;Sasser, AK; | Enumeration and characterization of circulating multiple myeloma cells in patients with plasma cell disorders | Br. J. Haematol. | 29105742 | Verification cohort samples were obtained from Conversant Biologics (Huntsville, AL, USA) under a biospecimen purchase agreement. These samples represent a heterogenous cohort of patients with active disease and those in remission | Full Paper |
2018 | Pekcec, A;Schülert, N;Stierstorfer, B;Deiana, S;Dorner-Ciossek, C;Rosenbrock, H; | Targeting the dopamine D1 receptor or its downstream signalling by inhibiting phosphodiesterase-1 improves cognitive performance | Br. J. Pharmacol. | 29726015 | paraffin blocks. Human brain tissue from healthy donors (Folio Biosciences, Ohio, USA) was fixed in 4% paraformaldehyde, and samples containing PFC were identified, trimmed and processed into paraffin blocks. All procurement | Full Paper |
2018 | Maity, A;Mick, R;Huang, AC;George, SM;Farwell, MD;Lukens, JN;Berman, AT;Mitchell, TC;Bauml, J;Schuchter, LM;O'Hara, M;Lin, LL;Demichele, A;Christodouleas, JP;Haas, NB;Patsch, DM;Hahn, SM;Minn, AJ;Wherry, EJ;Vonderheide, RH; | A phase I trial of pembrolizumab with hypofractionated radiotherapy in patients with metastatic solid tumours | Br. J. Cancer | 30318516 | ange in volume of the irradiated (index) lesion was also calculated, but this was not used in the RECIST measurement. Immunohistochemical staining PD-L1 staining was performed by an outside company (QualTek Molecular Laboratories, Newtown, PA, USA) | Full Paper |
2018 | Mehra, R;Seiwert, TY;Gupta, S;Weiss, J;Gluck, I;Eder, JP;Burtness, B;Tahara, M;Keam, B;Kang, H;Muro, K;Geva, R;Chung, HC;Lin, CC;Aurora-Garg, D;Ray, A;Pathiraja, K;Cheng, J;Chow, LQM;Haddad, R; | Efficacy and safety of pembrolizumab in recurrent/metastatic head and neck squamous cell carcinoma: pooled analyses after long-term follow-up in KEYNOTE-012 | Br. J. Cancer | 29955135 | PD-L1 expression status during screening was determined using a prototype PD-L1 immunohistochemical assay 18 performed at a laboratory site (QualTek) accredited by the College of American Pathologists and Clinical Laboratory Improvement Amendments, a | Full Paper |
2018 | Wein, L;Luen, SJ;Savas, P;Salgado, R;Loi, S; | Checkpoint blockade in the treatment of breast cancer: current status and future directions | Br. J. Cancer | 29808015 | Patients were required to be PD-L1 positive by immunohistochemistry (IHC), which was defined as PD-L1 positivity in the stroma or ≥1% tumour cells using their Qualtek assay.28 Most patients were heavily pre-treated; the median number of previous line | Full Paper |
2018 | Kanter, J;Tisdale, J;Kwiatkowski, J;Krishnamurti, L;Mapara, M;Schmidt, M;Miller, A;Pierciey, F;Shi, W;Ribeil, J;Walters, M;Thompson, A; | Outcomes for Initial Patient Cohorts with up to 33 Months of Follow-up in the Hgb-206 Phase 1 Trial | Blood | Background Sickle cell disease (SCD) is a progressively debilitating genetic disease causing significant morbidity and early mortality for which a universal curative therapy is lacking. Expression of an anti-sickling β-globin via gene | Full Paper | |
2018 | Bosco, EE;Christie, RJ;Carrasco, R;Sabol, D;Zha, J;DaCosta, K;Brown, L;Kennedy, M;Meekin, J;Phipps, S;Ayriss, J;Du, Q;Bezabeh, B;Chowdhury, P;Breen, S;Chen, C;Reed, M;Hinrichs, M;Zhong, H;Xiao, Z;Dixit, R;Herbst, R;Tice, DA; | Preclinical evaluation of a GFRA1 targeted antibody-drug conjugate in breast cancer | Oncotarget | 29796165 | ... Fisher Scientific, Waltham, MA, USA) were coated with 5 mg/ml 9B3 antibody at 4○C overnight, washed, and blocked with 1% bovine serum albumin (Sigma-Aldrich, St Louis, MO). Human serum samples from Conversant Bio (Huntsville, AL) or from cell-lin | Full Paper |
2018 | Xu, Z;Wang, S;Li, Y;Zhu, F;Huang, J; | PRIM: An Efficient Preconditioning Iterative Reweighted Least Squares Method for Parallel Brain MRI Reconstruction | Neuroinformatics | 29423650 | International Holdings Inc. Albin, Mary, Westinghouse Alexander, William, Qualtek Manufacturing, Inc. Alexander, Chuck, Solid Concepts, Inc. Alexopoulos, Nick, Broadcom Corporation Alford, Charles, UES, Inc. Alvarado, Igor | Full Paper |
2018 | Locatelli, F;Walters, M;Kwiatkowski, J;Porter, J;Sauer, M;Thuret, I;Hongeng, S;Kulozik, A;Lal, A;Thrasher, A;Yannaki, E;Elliot, H;Tao, G;Asmal, M;Thompson, A; | Lentiglobin Gene Therapy for Patients with Transfusion-Dependent β-Thalassemia (TDT): Results from the Phase 3 Northstar-2 and Northstar-3 Studies | Blood | Background Transfusion-dependent β-thalassemia (TDT) is a severe genetic disease caused by impaired β-globin production, leading to severe anemia, lifelong transfusion dependence with iron overload and serious comorbidities. Gene thera | Full Paper | |
2018 | Li, C;Mathews, V;George, B;Kim, S;Hebert, K;Jiang, H;Li, C;Zhu, Y;Keesler, D;Agarwal, R;Boelens, J;Dvorak, C;Auletta, J;Goyal, R;Hanna, R;Kasow, K;Margolis, D;Shenoy, S;Walters, M;Eapen, M; | Related and Unrelated Donor Transplantation for β Thalassemia Major: Results of an International Survey | Blood | We studied 1110 children, adolescents and young adults with β thalassemia major transplanted with grafts from HLA-matched relative (n=677; 61%), HLA-mismatched relative (n=78; 7%), HLA-matched unrelated (n=252; 23%) and HLA-mismatched unrelated (n=10 | Full Paper | |
2018 | Rasko, J;Thompson, A;Kwiatkowski, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;Schmidt, M;Kletzel, M;Leboulch, P;Vichinsky, E;Deary, B;Chen, Y;Asmal, M;Walters, M; | Clinical Outcomes of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia Following Completion of the Northstar HGB-204 Study | Blood | Background Advances in red blood cell (RBC) transfusion and chelation have improved the prognosis of patients with transfusion-dependent β-thalassemia (TDT); however, many patients experience organ damage due to iron overload and other | Full Paper | |
2018 | Godwin, RL;Opatova, V;Garrison, NL;Hamilton, CA;Bond, JE; | Phylogeny of a cosmopolitan family of morphologically conserved trapdoor spiders (Mygalomorphae, Ctenizidae) using Anchored Hybrid Enrichment, with a description of the family, Halonoproctidae Pocock 1901 | Mol. Phylogenet. Evol. | 29656103 | Phylogeny of a cosmopolitan family of morphologically conserved trapdoor spiders (Mygalomorphae, Ctenizidae) using Anchored Hybrid Enrichment, with a description of the family, Halonoproctidae Pocock 1901 | Full Paper |
2018 | Hansen, R;Firdaus, SJ;Li, S;Janes, MR;Zhang, J;Liu, Y;Zarrinkar, PP; | An Internally Controlled Quantitative Target Occupancy Assay for Covalent Inhibitors | Sci Rep | 30254226 | spectrometry. Clinical specimens. Clinical FFPE specimens were purchased from Conversant Bio (Huntsville, AL) and Asterand Bioscience (Detroit, MI). Conversant and Asterand comply with all applicable regulations. Animal studies | Full Paper |
2018 | Potts, AH;Leng, Y;Babitzke, P;Romeo, T; | Examination of Csr regulatory circuitry using epistasis analysis with RNA-seq (Epi-seq) confirms that CsrD affects gene expression via CsrA, CsrB and CsrC | Sci Rep | 29599472 | RNA-seq libraries were then generated with the Stranded RNA-Seq Library Preparation Kit for Illumina (KAPA) and NEBNext Multiplex Oligos for Illumina adaptors (NEB) according to the manufacturer’s instructions for 100 ng of starting material and a me | Full Paper |
2018 | Friedman, KM;Garrett, TE;Evans, JW;Horton, HM;Latimer, HJ;Seidel, SL;Horvath, CJ;Morgan, RA; | Effective Targeting of Multiple B-Cell Maturation Antigen-Expressing Hematological Malignances by Anti-B-Cell Maturation Antigen Chimeric Antigen Receptor T Cells | Hum. Gene Ther. | 29641319 | LLC. Peripheral blood mononuclear cells (PBMCs) were isolated and cryopreserved in the authors' laboratory. Whole blood from two CLL patients was obtained from Conversant Biologics, Inc. Immunohistochemistry. Twenty | Full Paper |
2018 | Vasanthavel, S;Meenakshi, K;Nivedha, V;Ballamurugan, AM;Kannan, S; | Tuning the structural and mechanical properties in ZrO2-SiO2 binary system through Y3+ inclusions | Mater Sci Eng C Mater Biol Appl | 29519433 | Zirconia (ZrO 2 ) and Alumina (Al 2 O 3 ) are conversant bio-inert ceramics known for their superior mechanical properties while hydroxyapatite [Ca 5 (PO 4 ) 3 OH, HAP], β-tricalciumphosphate [β-Ca 3 (PO 4 ) 2 , β-TCP] and SiO 2 based bioactive glass | Full Paper |
2018 | Schlegel, C;Lapierre, LA;Weis, VG;Williams, JA;Kaji, I;Pinzon-Guzman, C;Prasad, N;Boone, B;Jones, A;Correa, H;Levy, SE;Han, X;Wang, M;Thomsen, K;Acra, S;Goldenring, JR; | Reversible deficits in apical transporter trafficking associated with deficiency in diacylglycerol acyltransferase | Traffic | 30095213 | Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee.++Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee.++Department of Surgery, Vanderbilt University School of Medicine, Nashville | Full Paper |
2018 | Selvan, N;George, S;Serajee, FJ;Shaw, M;Hobson, L;Kalscheuer, V;Prasad, N;Levy, SE;Taylor, J;Aftimos, S;Schwartz, CE;Huq, AM;Gecz, J;Wells, L; | O-GlcNAc transferase missense mutations linked to X-linked intellectual disability deregulate genes involved in cell fate determination and signaling | J. Biol. Chem. | 29769320 | From the Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602.++From the Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, Universit | Full Paper |
2018 | Edwardson, CF;Hollibaugh, JT; | Composition and Activity of Microbial Communities along the Redox Gradient of an Alkaline, Hypersaline, Lake | Front Microbiol | 29445359 | Samples were pooled and sequenced on one lane of an Illumina HiSeq2500 in Rapid Run mode with the 150PE (300 cycle) kit at HudsonAlpha Genomic Services Lab (Huntsville, AL). Bioinformatics of 16S rRNA Sequences | Full Paper |
2018 | Xu, J;Su, X;Bonizzoni, M;Zhong, D;Li, Y;Zhou, G;Nguyen, H;Tong, S;Yan, G;Chen, XG; | Comparative transcriptome analysis and RNA interference reveal CYP6A8 and SNPs related to pyrethroid resistance in Aedes albopictus | PLoS Negl Trop Dis | 30418967 | ; RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). A total of 24 cDNA libraries were prepared individually from 12 susceptible and 12 resistant mosqu | Full Paper |
2018 | Biase, FH;Kimble, KM; | Functional signaling and gene regulatory networks between the oocyte and the surrounding cumulus cells | BMC Genomics | 29747587 | The samples were subjected to 16 cycles of PCR amplification. For all samples, we used 1 ng of amplified cDNA for library preparation with the Nextera XT DNA library preg Kit (Illumina, Inc.), as per the procedures described in the SMART-seq2 protoco | Full Paper |
2018 | Dickinson, SE;Griffin, BA;Elmore, MF;Kriese-Anderson, L;Elmore, JB;Dyce, PW;Rodning, SP;Biase, FH; | Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potential | BMC Genomics | 29426285 | Libraries were prepared with the TruSeq Stranded mRNA Library Prep kit (Illumina, Inc., San Diego, CA) following manufacturer’s instructions. Libraries were quantified with Qubit™ dsDNA High Sensitivity Assay Kit (Eurogene, OR) and quality was evalua | Full Paper |
2018 | Lin, EM;Gong, J;Klempner, SJ;Chao, J; | Advances in immuno-oncology biomarkers for gastroesophageal cancer: Programmed death ligand 1, microsatellite instability, and beyond | World J. Gastroenterol. | 29991874 | Presence of tumor PD-L1 expression was a requirement for enrollment, with 40% of the patients screened demonstrating PD-L1-positive tumors. PD-L1 expression was detected using the 22C3 antibody with a prototype assay using QualTek or Dako platforms | Full Paper |
2018 | Fan, J;Lerner, J;Wyatt, MK;Cai, P;Peterson, K;Dong, L;Wistow, G; | The klotho-related protein KLPH (lctl) has preferred expression in lens and is essential for expression of clic5 and normal lens suture formation | Exp. Eye Res. | 29425878 | XAR film at −70 °C. 2.3. In situ hybridization (ISH). Frozen tissues were cut into 10 μm sections, mounted on gelatin-coated slides and stored at −80 °C. ISH was performed at Phylogeny Inc. (Columbus, OH). Slides were fixed in | Full Paper |
2018 | Hiatt, SM;Amaral, MD;Bowling, KM;Finnila, CR;Thompson, ML;Gray, DE;Lawlor, JMJ;Cochran, JN;Bebin, EM;Brothers, KB;East, KM;Kelley, WV;Lamb, NE;Levy, SE;Lose, EJ;Neu, MB;Rich, CA;Simmons, S;Myers, RM;Barsh, GS;Cooper, GM; | Systematic reanalysis of genomic data improves quality of variant interpretation | Clin. Genet. | 29652076 | HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama. | Full Paper |
2018 | Håvik, AL;Bruland, O;Aarhus, M;Kalland, KH;Stokowy, T;Lund-Johansen, M;Knappskog, PM; | Screening for viral nucleic acids in vestibular schwannoma | J. Neurovirol. | 30168016 | ; RNA sequencing on RNA extracted from five sVS tissue biopsies was performed by HudsonAlpha (HudsonAlpha Genomic Services Laboratory, 601 Genome Way, Huntsville, AL 35806 USA) using their "Poly A RNA-sequencing" service including 50 cycles of paire | Full Paper |
2018 | Mohan, S;Greenstein, I;Ng, C;Frazier, K;Nguyen, G;Harding, L;Barlow, D; | Assessing N w-hydroxy-L-arginine applicability as a novel ethnic specific estrogen-negative breast cancer marker | Amino Acids | 29260322 | Collection (Manassas, VA). We received de-identified plasma samples from Cooperative Human Tissue Network (Philadelphia, PA), Tissue-for-Research (Atlanta, GA), and Conversant Bio (Huntsville, AL). The de-identified | Full Paper |
2018 | Zhu, J;Gan, X;Ao, Q;Shen, X;Tan, Y;Chen, M;Luo, Y;Wang, H;Jiang, H;Li, C; | Basal polarization of the immune responses to Streptococcus agalactiae susceptible and resistant tilapia (Oreochromis niloticus) | Fish Shellfish Immunol. | 29454032 | ; For each timepoint, equal amounts of RNA from the three replicates were pooled for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) | Full Paper |
2018 | Porter, ET;Porter, FS; | A Strain Gauge Monitor (SGM) for Continuous Valve Gape Measurements in Bivalve Molluscs in Response to Laboratory Induced Diel-cycling Hypoxia and pH | J Vis Exp | 30124658 | Name Company Catalog Number Comments Campbell CR 10x data logger Campbell Scientific, Logan, Utah Or other data logger. At Campbell the CR 10X has been replaced with the CR 1000 Campbell CR 10x multiplexer Campbell Scientific, Logan, Utah Data logg | Full Paper |
2018 | Yang, Y;Fu, Q;Liu, Y;Wang, X;Dunham, R;Liu, S;Bao, L;Zeng, Q;Zhou, T;Li, N;Qin, Z;Jiang, C;Gao, D;Liu, Z; | Comparative transcriptome analysis reveals conserved branching morphogenesis related genes involved in chamber formation of catfish swimbladder | Physiol. Genomics | 29167198 | Equal amounts of RNA from the three replicates for each sample were pooled together for RNA-Seq library preparation. The remaining replicate pools were preserved as biological replicates for quantitative (q)RT-PCR validation. The cDNA libraries were | Full Paper |
2018 | Cohen, RB;Delord, JP;Doi, T;Piha-Paul, SA;Liu, SV;Gilbert, J;Algazi, AP;Damian, S;Hong, RL;Le Tourneau, C;Day, D;Varga, A;Elez, E;Wallmark, J;Saraf, S;Thanigaimani, P;Cheng, J;Keam, B; | Pembrolizumab for the Treatment of Advanced Salivary Gland Carcinoma: Findings of the Phase 1b KEYNOTE-028 Study | Am. J. Clin. Oncol. | 29462123 | Tumor PD-L1 positivity was determined at a central laboratory using formalin-fixed archived or newly obtained biopsy samples and a prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA),17 based on the 22C3 antibody (Merck & | Full Paper |
2018 | Glover, ME;McCoy, CR;Shupe, EA;Unroe, KA;Jackson, NL;Clinton, SM; | Perinatal exposure to the SSRI paroxetine alters the methylome landscape of the developing dentate gyrus | Eur. J. Neurosci. | 30585666 | ; quantified (Quant-iT high sensitivity DNA Assay Kit, Invitrogen, Grand Island, NY). Next- generation sequencing was performed at HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) using NEBNext reagents (New Engla | Full Paper |
2018 | Verwimp, C;Ruttink, T;Muylle, H;Van Glabeke, S;Cnops, G;Quataert, P;Honnay, O;Roldán-Ruiz, I; | Temporal changes in genetic diversity and forage yield of perennial ryegrass in monoculture and in combination with red clover in swards | PLoS ONE | 30408053 | The protocol for the 112 tissue pool samples was slightly adjusted, based on the results of the validation step. In this case, the 50 μL bead-purified ligate was split into three aliquots, and 16.7 μL was used for three replicate PCR reactions to acc | Full Paper |
2018 | Geister, KA;Timms, AE;Beier, DR; | Optimizing Genomic Methods for Mapping and Identification of Candidate Variants in ENU Mutagenesis Screens Using Inbred Mice | G3 (Bethesda) | 29208648 | ... centrifugation. DNA was tested at SCRI for purity and quality using a Nanodrop spectrophotometer and the Qubit dsDNA Broad Range Assay (#Q32850; Thermo Fisher Scientific). DNA samples were shipped to HudsonAlpha Genomic Services Laboratory (Hunts | Full Paper |
2018 | Papanek, B;O'Dell, KB;Manga, P;Giannone, RJ;Klingeman, DM;Hettich, RL;Brown, SD;Guss, AM; | Transcriptomic and proteomic changes from medium supplementation and strain evolution in high-yielding Clostridium thermocellum strains | J. Ind. Microbiol. Biotechnol. | 30187243 | ; Sequencing was completed using a SR50 sequencing protocol on an Illumina HiSeq 2500 platform (HudsonAlpha Genomic Services Laboratory; Huntsville, AL, USA). RNA-Seq analysis. The analysis of the raw RNA-Seq data was performed as previously describ | Full Paper |
2018 | Ziai, J;Gilbert, HN;Foreman, O;Eastham-Anderson, J;Chu, F;Huseni, M;Kim, JM; | CD8+ T cell infiltration in breast and colon cancer: A histologic and statistical analysis | PLoS ONE | 29320521 | IV were represented. Colorectal cancer samples were procured from Conversant Bio and included moderately differentiated adenocarcinoma (9), mucinous carcinoma (1) and rectal (2) carcinoma cases. Samples contained | Full Paper |
2018 | Gallery, M;Zhang, J;Bradley, DP;Brauer, P;Cvet, D;Estevam, J;Danaee, H;Greenfield, E;Li, P;Manfredi, M;Loke, HK;Rabino, C;Stringer, B;Williamson, M;Wyant, T;Yang, J;Zhu, Q;Abu-Yousif, A;Veiby, OP; | A monomethyl auristatin E-conjugated antibody to guanylyl cyclase C is cytotoxic to target-expressing cells in vitro and in vivo | PLoS ONE | 29370189 | scanning system. Colon tissue microarrays (US Biomax) were stained and scored by two independent pathologists following a protocol developed at Qualtek Molecular Laboratories on their Tek-Mate automated stainer. Briefly ;ith hematoxylin and imag | Full Paper |
2018 | Lagatie, O;Verheyen, A;Nijs, E;Van Dorst, B;Batsa Debrah, L;Debrah, A;Supali, T;Sartono, E;Stuyver, LJ; | Evaluation of the Diagnostic Performance of Onchocerca volvulus Linear Epitopes in a Peptide Enzyme-Linked Immunosorbent Assay | Am. J. Trop. Med. Hyg. | 29313477 | (Glasgow, Scotland). A sec- ond healthy control sample set was composed of 49 plasma samples from healthy individuals from Belgium.20-24 Further- more, different sets of plasma samples were obtained from Discovery Life Sciences, Inc | Full Paper |
2018 | Andreotta, PW;Arold, S;Kenyon, J;Spicer, D;Woodman, P;Berry, E;Brogan, T;Kong, S;Okerholm, P;Russell, V;Clarke, RW;Hava, DL; | Inhaled calcium salts inhibit tobacco smoke-induced inflammation by modulating expression of chemokines and cytokines | Pulm Pharmacol Ther | 30359782 | Secreted protein expression and gene expression were assayed 2 h after LPS stimulation. 2.5. Human macrophage cultures. Human CD14 + monocytes were purchased from Conversant Biologics and differentiated into macrophages in culture | Full Paper |
2018 | Fall, L;Clevenger, J;McGregor, C; | Assay development and marker validation for marker assisted selection of Fusarium oxysporum f. sp. niveum race 1 in watermelon | Mol Breeding | for sequencing. Library construction and whole genome Illumina sequencing on an Illumina HiSeq X (Illumina, Inc., San Diego, CA) were performed at HudsonAlpha Genomic services laboratory (Huntsville, AL). Constructed | Full Paper | |
2018 | Zobel-Thropp, PA;Bulger, EA;Cordes, MHJ;Binford, GJ;Gillespie, RG;Brewer, MS; | Sexually dimorphic venom proteins in long-jawed orb-weaving spiders (Tetragnatha) comprise novel gene families | PeerJ | 29876146 | RNA extractions from the BF population of T. versicolor males (two) and females (10) were shipped to the Genomic Services Lab at HudsonAlpha (Huntsville, AL, USA), where cDNA libraries were prepared from total RNA (poly-A isolation, Illumina TruSeq R | Full Paper |
2018 | Awad, H;Bratasz, A;Nuovo, G;Burry, R;Meng, X;Kelani, H;Brown, M;Ramadan, ME;Williams, J;Bouhliqah, L;Popovich, PG;Guan, Z;Mcallister, C;Corcoran, SE;Kaspar, B;Michele Basso, D;Otero, JJ;Kirsch, C;Davis, IC;Croce, CM;Michaille, JJ;Tili, E; | MiR-155 deletion reduces ischemia-induced paralysis in an aortic aneurysm repair mouse model: Utility of immunohistochemistry and histopathology in understanding etiology of spinal cord paralysis | Ann Diagn Pathol | 29966831 | The degree of permeability was determined by measuring the fluorescence of the receiver plate well solution. 2.5. MicroRNA in situ hybridization (ISH). ISH for miR-155 was performed by Phylogeny Inc. (Powell, OH), as previously described [16] | Full Paper |
2018 | Dufu, K;Patel, M;Oksenberg, D;Cabrales, P; | GBT440 improves red blood cell deformability and reduces viscosity of sickle cell blood under deoxygenated conditions | Clin. Hemorheol. Microcirc. | 29660913 | The study was approved by the IRB of the University of North Carolina (UNC) Sickle Cell program [IRB # 88-0343 (GCRC-101)]. Blood from trait individuals (AS) collected in sodium citrate vacutainers was purchased from Conversant Bio, Inc. (Huntsville, | Full Paper |
2018 | Smyth, E;Thuss-Patience, PC; | Immune Checkpoint Inhibition in Gastro-Oesophageal Cancer | Oncol Res Treat | 29705787 | gastro-oesophageal cancer. Unlike the studies with nivolumab, in the KEYNOTE- 12 patients were selected for PD-L1-positive status; however, a different antibody (QualTek 22C3) and scoring system were used. Eligibility was | Full Paper |
2018 | Yuan, Z;Huang, W;Liu, S;Xu, P;Dunham, R;Liu, Z; | Historical demography of common carp estimated from individuals collected from various parts of the world using the pairwise sequentially markovian coalescent approach | Genetica | 29299799 | ; Qiagen). Genome re-sequencing and sequence alignment. DNA library preparation and sequencing were conducted at HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA) following manufacturer's instructions | Full Paper |
2018 | Vakulskas, CA;Dever, DP;Rettig, GR;Turk, R;Jacobi, AM;Collingwood, MA;Bode, NM;McNeill, MS;Yan, S;Camarena, J;Lee, CM;Park, SH;Wiebking, V;Bak, RO;Gomez-Ospina, N;Pavel-Dinu, M;Sun, W;Bao, G;Porteus, MH;Behlke, MA; | A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells | Nature medicine | 30082871 | ere used from either fresh cord blood (CB) (kindly provided by Binns Family program for Cord Blood Research), isolated from the peripheral blood of SCD patients, or frozen plerixafor mobilized CD34+ (AllCells). HSPCs were cultured at 250,000-500,000 | Full Paper |
2018 | Good, Z;Sarno, J;Jager, A;Samusik, N;Aghaeepour, N;Simonds, EF;White, L;Lacayo, NJ;Fantl, WJ;Fazio, G;Gaipa, G;Biondi, A;Tibshirani, R;Bendall, SC;Nolan, GP;Davis, KL; | Single-cell developmental classification of B cell precursor acute lymphoblastic leukemia at diagnosis reveals predictors of relapse | Nature medicine | 29505032 | Reagents and methods relevant to this work can also be found in the Life Sciences Reporting Summary. Fresh human bone marrow was obtained from healthy adult donors (n = 5; 3 females: 20, 27, 44 y.o., 2 males: 26, 28 y.o., average: 29 y.o.; AllCells, | Full Paper |
2018 | Deng, M;Gui, X;Kim, J;Xie, L;Chen, W;Li, Z;He, L;Chen, Y;Chen, H;Luo, W;Lu, Z;Xie, J;Churchill, H;Xu, Y;Zhou, Z;Wu, G;Yu, C;John, S;Hirayasu, K;Nguyen, N;Liu, X;Huang, F;Li, L;Deng, H;Tang, H;Sadek, AH;Zhang, L;Huang, T;Zou, Y;Chen, B;Zhu, H;Arase, H;Xia, N;Jiang, Y;Collins, R;You, MJ;Homsi, J;Unni, N;Lewis, C;Chen, GQ;Fu, YX;Liao, XC;An, Z;Zheng, J;Zhang, N;Zhang, CC; | LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration | Nature | 30333625 | ockout was validated by flow cytometry. Leukemia cell and T cell co-culture assay In the co-culture assay, human T cells (5 × 104 per well) isolated from health donor peripheral blood (PB009-1-0, Allcells) were mixed with irradiated (28 Gy) indicate | Full Paper |
2018 | Roth, TL;Puig-Saus, C;Yu, R;Shifrut, E;Carnevale, J;Li, PJ;Hiatt, J;Saco, J;Krystofinski, P;Li, H;Tobin, V;Nguyen, DN;Lee, MR;Putnam, AL;Ferris, AL;Chen, JW;Schickel, JN;Pellerin, L;Carmody, D;Alkorta-Aranburu, G;Del Gaudio, D;Matsumoto, H;Morell, M;Mao, Y;Cho, M;Quadros, RM;Gurumurthy, CB;Smith, B;Haugwitz, M;Hughes, SH;Weissman, JS;Schumann, K;Esensten, JH;May, AP;Ashworth, A;Kupfer, GM;Greeley, SAW;Bacchetta, R;Meffre, E;Roncarolo, MG;Romberg, N;Herold, KC;Ribas, A;Leonetti, MD;Marson, A; | Reprogramming human T cell function and specificity with non-viral genome targeting | Nature | 29995861 | Additional leukapheresis products from healthy donors were collected either under UCLA Institutional Review Board (IRB) approval #10-001598 or purchased from AllCells, LLC. All Save | Full Paper |
2018 | Butler, A;Hoffman, P;Smibert, P;Papalexi, E;Satija, R; | Integrating single-cell transcriptomic data across different conditions, technologies, and species | Nature biotechnology | 29608179 | We therefore repeated the original experiment, where PBMCs from a healthy human donor (ALLCELLS) were cultured with RPMI medium supplemented with 10% FBS and stimulated Save | Full Paper |
2018 | Neal, JT;Li, X;Zhu, J;Giangarra, V;Grzeskowiak, CL;Ju, J;Liu, IH;Chiou, SH;Salahudeen, AA;Smith, AR;Deutsch, BC;Liao, L;Zemek, AJ;Zhao, F;Karlsson, K;Schultz, LM;Metzner, TJ;Nadauld, LD;Tseng, YY;Alkhairy, S;Oh, C;Keskula, P;Mendoza-Villanueva, D;De La Vega, FM;Kunz, PL;Liao, JC;Leppert, JT;Sunwoo, JB;Sabatti, C;Boehm, JS;Hahn, WC;Zheng, GXY;Davis, MM;Kuo, CJ; | Organoid Modeling of the Tumor Immune Microenvironment | Cell | 30550791 | PBMCs, Pan T and CD19 + B cells used for the technical validation part of the immune profiling solution were obtained from AllCells (Catalog #: PB001, PB009-1F and PB010-0). Tumor Save | Full Paper |
2018 | Buenrostro, JD;Corces, MR;Lareau, CA;Wu, B;Schep, AN;Aryee, MJ;Majeti, R;Chang, HY;Greenleaf, WJ; | Integrated Single-Cell Analysis Maps the Continuous Regulatory Landscape of Human Hematopoietic Differentiation | Cell | 29706549 | Healthy adult bone marrow allcells https://www.allcells.com/products/whole-bone-marrow- Samples were collected commercially from allcells. Further information about the donors Save | Full Paper |
2018 | Su, R;Dong, L;Li, C;Nachtergaele, S;Wunderlich, M;Qing, Y;Deng, X;Wang, Y;Weng, X;Hu, C;Yu, M;Skibbe, J;Dai, Q;Zou, D;Wu, T;Yu, K;Weng, H;Huang, H;Ferchen, K;Qin, X;Zhang, B;Qi, J;Sasaki, AT;Plas, DR;Bradner, JE;Wei, M;Marcucci, G;Jiang, X;Mulloy, JC;Jin, J;He, C;Chen, J; | R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling | Cell | 29249359 | R-2-hydroxyglutarate (R-2HG), produced at high levels by mutant isocitrate dehydrogenase 1/2 (IDH1/2) enzymes, was reported as an oncometabolite. We show here that R-2HG also Save | Full Paper |
2018 | Choi, HW;Suwanpradid, J;Kim, IH;Staats, HF;Haniffa, M;MacLeod, AS;Abraham, SN; | Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles | Science (New York, N.Y.) | 30409859 | 100 U/ml penicillin, and 100 μg/ml streptomycin was utilized. In brief, purified peripheral blood CD34+ peripheral blood cells (Allcells) were suspended at 5×105 cells/ml in supplemented IMDM containing 100 ng/ml recombinant human (rh) SCF, 10 ng/ml | Full Paper |
2018 | Ting, PY;Parker, AE;Lee, JS;Trussell, C;Sharif, O;Luna, F;Federe, G;Barnes, SW;Walker, JR;Vance, J;Gao, MY;Klock, HE;Clarkson, S;Russ, C;Miraglia, LJ;Cooke, MP;Boitano, AE;McNamara, P;Lamb, J;Schmedt, C;Snead, JL; | Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cells | Nature methods | 30297964 | AllCells and Lonza also obtained samples from outside sources with documented IRB compliance. Lot numbers can be found in the Nature Research Reporting Summary. Save | Full Paper |
2018 | Jenkins, RW;Aref, AR;Lizotte, PH;Ivanova, E;Stinson, S;Zhou, CW;Bowden, M;Deng, J;Liu, H;Miao, D;He, MX;Walker, W;Zhang, G;Tian, T;Cheng, C;Wei, Z;Palakurthi, S;Bittinger, M;Vitzthum, H;Kim, JW;Merlino, A;Quinn, M;Venkataramani, C;Kaplan, JA;Portell, A;Gokhale, PC;Phillips, B;Smart, A;Rotem, A;Jones, RE;Keogh, L;Anguiano, M;Stapleton, L;Jia, Z;Barzily-Rokni, M;Cañadas, I;Thai, TC;Hammond, MR;Vlahos, R;Wang, ES;Zhang, H;Li, S;Hanna, GJ;Huang, W;Hoang, MP;Piris, A;Eliane, JP;Stemmer-Rachamimov, AO;Cameron, L;Su, MJ;Shah, P;Izar, B;Thakuria, M;LeBoeuf, NR;Rabinowits, G;Gunda, V;Parangi, S;Cleary, JM;Miller, BC;Kitajima, S;Thummalapalli, R;Miao, B;Barbie, TU;Sivathanu, V;Wong, J;Richards, WG;Bueno, R;Yoon, CH;Miret, J;Herlyn, M;Garraway, LA;Van Allen, EM;Freeman, GJ;Kirschmeier, PT;Lorch, JH;Ott, PA;Hodi, FS;Flaherty, KT;Kamm, RD;Boland, GM;Wong, KK;Dornan, D;Paweletz, CP;Barbie, DA; | Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids | Cancer discovery | 29101162 | Freshly isolated human CD4+ and CD8+ T cells were obtained from AllCells. Cells were spun down and resuspended in serum free X-vivo15 media (Lonza Walkersville, Inc.) supplemented with 5 ng/mL IL17 and incubated overnight at 37°C. Cells were plated o | Full Paper |
2018 | Ryu, JK;Rafalski, VA;Meyer-Franke, A;Adams, RA;Poda, SB;Rios Coronado, PE;Pedersen, LØ;Menon, V;Baeten, KM;Sikorski, SL;Bedard, C;Hanspers, K;Bardehle, S;Mendiola, AS;Davalos, D;Machado, MR;Chan, JP;Plastira, I;Petersen, MA;Pfaff, SJ;Ang, KK;Hallenbeck, KK;Syme, C;Hakozaki, H;Ellisman, MH;Swanson, RA;Zamvil, SS;Arkin, MR;Zorn, SH;Pico, AR;Mucke, L;Freedman, SB;Stavenhagen, JB;Nelson, RB;Akassoglou, K; | Fibrin-targeting immunotherapy protects against neuroinflammation and neurodegeneration | Nature immunology | 30323343 | wed by an incubation of 6.88 μM CD11b I-domain for 2 h at 25 ˚C. Cell Culture. Primary microglia and BMDMs were isolated, cultured, and treated with fibrin or LPS as previously described16. PBMCs (AllCells) (2×106 cells/mL) were plated in RPMI-1640 | Full Paper |
2018 | Chorzalska, A;Morgan, J;Ahsan, N;Treaba, DO;Olszewski, AJ;Petersen, M;Kingston, N;Cheng, Y;Lombardo, K;Schorl, C;Yu, X;Zini, R;Pacilli, A;Tepper, A;Coburn, J;Hryniewicz-Jankowska, A;Zhao, TC;Oancea, E;Reagan, JL;Liang, O;Kotula, L;Quesenberry, PJ;Gruppuso, PA;Manfredini, R;Vannucchi, AM;Dubielecka, PM; | Bone marrow-specific loss of ABI1 induces myeloproliferative neoplasm with features resembling human myelofibrosis | Blood | 30213875 | CD34 + cells were isolated from the bone marrow of patients with PMF or from healthy marrow purchased from AllCells (Alameda, CA). Granulocytes were isolated from peripheral Save | Full Paper |
2018 | Cairo, M;Parsons, S;Braniecki, S;Talano, J;Moore, T;Shi, Q;Keever-Taylor, C;Weinberg, R;Grossman, B;Dozor, A;Friedman, D;McKinstry, R;Verbsky, J;Moorthy, C;Weidner, R;Rodday, A;Ayello, J;Semidei-Pomales, M;Flower, A;Chu, Y;Morris, E;Mahanti, H;Fabricatore, S;Militano, O;Klejmont, L;van de Ven, C;Baxter-Lowe, L;Vichinsky, E;Walters, M;Shenoy, S; | Significantly Improved Long Term Health Related Quality of Life (HRQL) and Neurocognition Following Familial Haploidentical Stem Cell Transplantation (HISCT) Utilizing CD34 Enrichment and Mononuclear (CD3) Addback in High Risk Patients with Sickle Cell Disease (SCD) | Blood | ViaCord Processing Lab: Other: Medical Director; AllCells Inc.: Other: Medical Director. Shenoy: Novartis, Vertex, Bluebird Bio: Honoraria, Membership on an entity's Board of Directors or advisory committees | Full Paper | |
2018 | Jamieson, CHM;Millan, MT;Creasey, AA;Lomax, G;Donohoe, ME;Walters, MC;Abedi, M;Bota, DA;Zaia, JA;Adams, JS; | CIRM Alpha Stem Cell Clinics: Collaboratively Addressing Regenerative Medicine Challenges | Cell stem cell | 29859170 | Declaration of Interests All authors have CIRM funding to declare. M.C.W. is a Consultant for Bioverativ, Inc., Bluebird Bio, Inc., and Sangamo Biotherapeutics and a Medical Director for AllCells, Inc. and ViaCord Processing Lab | Full Paper |
2018 | Cesana, M;Guo, MH;Cacchiarelli, D;Wahlster, L;Barragan, J;Doulatov, S;Vo, LT;Salvatori, B;Trapnell, C;Clement, K;Cahan, P;Tsanov, KM;Sousa, PM;Tazon-Vega, B;Bolondi, A;Giorgi, FM;Califano, A;Rinn, JL;Meissner, A;Hirschhorn, JN;Daley, GQ; | A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development | Cell stem cell | 29625070 | Human CD34 + cells (Lonza and AllCells) from all sources were obtained as viable frozen states. The ages of the individuals used for the profiling and treatments are as follows: FL- Save | Full Paper |
2018 | Kao, YR;Chen, J;Narayanagari, SR;Todorova, TI;Aivalioti, MM;Ferreira, M;Ramos, PM;Pallaud, C;Mantzaris, I;Shastri, A;Bussel, JB;Verma, A;Steidl, U;Will, B; | Thrombopoietin receptor-independent stimulation of hematopoietic stem cells by eltrombopag | Science translational medicine | 30209246 | Healthy cryopreserved human bone marrow cells were purchased from AllCells and ReachBio Research Labs; fresh unprocessed bone marrow cells were from Lonza. Samples of Save | Full Paper |
2018 | Kou, X;Xu, X;Chen, C;Sanmillan, ML;Cai, T;Zhou, Y;Giraudo, C;Le, A;Shi, S; | The Fas/Fap-1/Cav-1 complex regulates IL-1RA secretion in mesenchymal stem cells to accelerate wound healing | Science translational medicine | 29540618 | Human bone marrow aspirates from healthy human adult volunteers (20 to 35 years of age) were purchased from AllCells LLC. Human GMSCs and SMSCs were isolated from the Save | Full Paper |
2018 | Lai, D;Chen, M;Su, J;Liu, X;Rothe, K;Hu, K;Forrest, DL;Eaves, CJ;Morin, GB;Jiang, X; | PP2A inhibition sensitizes cancer stem cells to ABL tyrosine kinase inhibitors in BCR-ABL+ human leukemia | Science translational medicine | 29437150 | Overcoming drug resistance and targeting leukemic stem cells (LSCs) remain major challenges in curing BCR-ABL + human leukemia. Using an advanced drug/proliferation screen, we Save | Full Paper |
2018 | Ryan, DE;Taussig, D;Steinfeld, I;Phadnis, SM;Lunstad, BD;Singh, M;Vuong, X;Okochi, KD;McCaffrey, R;Olesiak, M;Roy, S;Yung, CW;Curry, B;Sampson, JR;Bruhn, L;Dellinger, DJ; | Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAs | Nucleic acids research | 29216382 | Nucleofector kit (V4SP-3096). Cells were harvested at 48 hr post-transfection. Mobilized human peripheral blood CD34-positive hematopoietic stem and progenitor cells (CD34+ HSPCs) were purchased from AllCells (Alameda, CA) and thawed per manufacturer | Full Paper |
2018 | Syed, SA;Beurel, E;Loewenstein, DA;Lowell, JA;Craighead, WE;Dunlop, BW;Mayberg, HS;Dhabhar, F;Dietrich, WD;Keane, RW;de Rivero Vaccari, JP;Nemeroff, CB; | Defective Inflammatory Pathways in Never-Treated Depressed Patients Are Associated with Poor Treatment Response | Neuron | 30146307 | PBMCs from a non-hispanic white female healthy donor (age 50, weight: 142 lbs, height 68 in) unmedicated for a week, were freshly isolated at ALLCELLS. No other information Save | Full Paper |
2018 | Lei, L;Chen, H;Xue, W;Yang, B;Hu, B;Wei, J;Wang, L;Cui, Y;Li, W;Wang, J;Yan, L;Shang, W;Gao, J;Sha, J;Zhuang, M;Huang, X;Shen, B;Yang, L;Chen, J; | APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks | Nature structural & molecular biology | 29323274 | Cryopreserved human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells, LLC (PB003F). CD8+ T cells were pre-enriched from the PBMCs with Easysep Human CD8+ T cell enrichment kit (19053, Stemcell Technologies) according to the man | Full Paper |
2018 | Stoeckius, M;Zheng, S;Houck-Loomis, B;Hao, S;Yeung, BZ;Mauck, WM;Smibert, P;Satija, R; | Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics | Genome biology | 30567574 | urther enable multiplexing strategies to generalize to diverse experiments regardless of species, tissue, or technology. Methods PBMC genotyping Peripheral blood mononuclear cells were obtained from AllCells (USA). Genomic DNA was purified using the | Full Paper |
2018 | Xu, Y;Zhao, W;Olson, SD;Prabhakara, KS;Zhou, X; | Alternative splicing links histone modifications to stem cell fate decision | Genome biology | 30217220 | After reaching ~ 70% confluence, the adherent cells were harvested at the passage 1 (P1) hAT-MSC/AEY239. hBM-MSCs were isolated from commercially available fresh human bone marrow (hBM) aspirates (AllCells, Emeryville, CA, USA) and expanded following | Full Paper |
2018 | Salas, LA;Koestler, DC;Butler, RA;Hansen, HM;Wiencke, JK;Kelsey, KT;Christensen, BC; | An optimized library for reference-based deconvolution of whole-blood biospecimens assayed using the Illumina HumanMethylationEPIC BeadArray | Genome biology | 29843789 | urity cell subtypes (neutrophils (Neu), monocytes (Mono), B lymphocytes (Bcells), T helper lymphocytes (CD4T), T cytotoxic lymphocytes (CD8T), and natural killer lymphocytes (NK)) were purchased from AllCells corporation (Alameda, CA, USA) and STEMC | Full Paper |
2018 | Lim, SH;Kathuria, H;Tan, JJY;Kang, L; | 3D printed drug delivery and testing systems - a passing fad or the future? | Advanced drug delivery reviews | 29778901 | Primary human dental pulp stem cells (DPSC) from a single donor (DPF003, AllCells, USA) were cultured in a complete growth media containing Dulbecco's Modified Eagle's Medium, supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. C | Full Paper |
2018 | Marabissi, D;Bartoli, G;Micciullo, L;Fantacci, R; | Energy efficient multicast beamforming in ultra dense networks | 2018 IEEE Wireless Communications and Networking Conference (WCNC) | This figure clearly shows the better behavior of our method in comparison with static approaches (ie, OnlyMacro, AllCells). Moreover, we can see that even if sub-optimum, our method is Save | Full Paper | |
2018 | Cao, L;Pauthner, M;Andrabi, R;Rantalainen, K;Berndsen, Z;Diedrich, JK;Menis, S;Sok, D;Bastidas, R;Park, SR;Delahunty, CM;He, L;Guenaga, J;Wyatt, RT;Schief, WR;Ward, AB;Yates, JR;Burton, DR;Paulson, JC; | Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer | Nature communications | 30209313 | Primary PBMC samples were obtained through the commercial vendor AllCells (Emeryville, CA The leukapak sample was obtained from a commercial vendor (AllCells) using a protocol Save | Full Paper |
2018 | Mezger, A;Klemm, S;Mann, I;Brower, K;Mir, A;Bostick, M;Farmer, A;Fordyce, P;Linnarsson, S;Greenleaf, W; | High-throughput chromatin accessibility profiling at single-cell resolution | Nature communications | 30194434 | , and B cells were isolated from whole blood (AllCells, CA, USA) using EasySep Direct Human All human cells were obtained from AllCells with explicit consent to publish data for broad Save | Full Paper |
2018 | Lyu, G;Guan, Y;Zhang, C;Zong, L;Sun, L;Huang, X;Huang, L;Zhang, L;Tian, XL;Zhou, Z;Tao, W; | TGF-β signaling alters H4K20me3 status via miR-29 and contributes to cellular senescence and cardiac aging | Nature communications | 29967491 | The culture medium was MEM supplemented with 10% FBS (GIBCO), and the culture dish was gelatin-coated before inoculation of primary human umbilical vascular endothelial cells (HUVECs) purchased from ALLCELLS Co. Ltd., Shanghai, China. Cells were test | Full Paper |
2018 | Xu, L;Gordon, R;Farmer, R;Pattanayak, A;Binkowski, A;Huang, X;Avram, M;Krishna, S;Voll, E;Pavese, J;Chavez, J;Bruce, J;Mazar, A;Nibbs, A;Anderson, W;Li, L;Jovanovic, B;Pruell, S;Valsecchi, M;Francia, G;Betori, R;Scheidt, K;Bergan, R; | Precision therapeutic targeting of human cancer cell motility | Nature communications | 29934502 | od cells, hemoglobin, and platelets. Hematopoietic stem cell colony formation assay Fourteen-day colony formation assays were conducted as described by us39, using human cord blood CD34+ stem cells (AllCells Inc.), using MethoCultExpress colony gro | Full Paper |
2018 | Yang, R;Yu, T;Kou, X;Gao, X;Chen, C;Liu, D;Zhou, Y;Shi, S; | Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter | Nature communications | 29858571 | (PFA, Sigma-Aldrich) after 16 days of culturing. Clusters with more than 50 cells were counted as colonies under microscopy. Isolation and culture of human BMMSCs We purchased human bone marrow from AllCells LLC (Alameda, CA, USA), which were aspira | Full Paper |
2018 | Lummertz da Rocha, E;Rowe, RG;Lundin, V;Malleshaiah, M;Jha, DK;Rambo, CR;Li, H;North, TE;Collins, JJ;Daley, GQ; | Reconstruction of complex single-cell trajectories using CellRouter | Nature communications | 29497036 | es) would act as perturbations that could be explored by CLR to identify putative regulatory relationships. In vitro erythroid differentiation Mobilized peripheral blood CD34+ stem/progenitor cells (AllCells) were obtained from three independent don | Full Paper |
2018 | Ravi, R;Noonan, KA;Pham, V;Bedi, R;Zhavoronkov, A;Ozerov, IV;Makarev, E;V Artemov, A;Wysocki, PT;Mehra, R;Nimmagadda, S;Marchionni, L;Sidransky, D;Borrello, IM;Izumchenko, E;Bedi, A; | Bifunctional immune checkpoint-targeted antibody-ligand traps that simultaneously disable TGFβ enhance the efficacy of cancer immunotherapy | Nature communications | 29467463 | )(4-8-week old) were irradiated at 200 cGy, followed by adoptive transfer of human BM CD34 + cells (7 × 10 4 per mouse) from a normal donor (HLA-matched to the tumor) (ALLCELLS) Save | Full Paper |
2018 | Bak, RO;Dever, DP;Porteus, MH; | CRISPR/Cas9 genome editing in human hematopoietic stem cells | Nature protocols | 29370156 | Fresh or frozen CD34 + human HSPCs (either acquired per institutional protocol or purchased from various commercial sources, including AllCells (CB-derived, cat. no. CB008F; mPB- Save | Full Paper |
2018 | Adine, C;Ng, KK;Rungarunlert, S;Souza, GR;Ferreira, JN; | Engineering innervated secretory epithelial organoids by magnetic three-dimensional bioprinting for stimulating epithelial growth in salivary glands | Biomaterials | 30025245 | Human dental pulp stem cells (hDPSCs) were commercially obtained from AllCells (Alameda, CA, USA) from three different donors. These stem cells were derived from the pulp tissues of exfoliated deciduous teeth, primary incisors, or permanent third mol | Full Paper |
2018 | Jiang, L;Zhang, W;Wei, L;Zhou, Q;Yang, G;Qian, N;Tang, Y;Gao, Y;Jiang, X; | Early effects of parathyroid hormone on vascularized bone regeneration and implant osseointegration in aged rats | Biomaterials | 29960821 | Human umbilical vein endothelial cells (HUVECs) (Allcells, Shanghai, China) were cultured and their in vitro recruitment and angiogenesis abilities were measured. PTH (1-34) was added at a concentration of 10 nM in the lower chambers of 24-well trans | Full Paper |
2018 | Odegaard, JI;Vincent, JJ;Mortimer, S;Vowles, JV;Ulrich, BC;Banks, KC;Fairclough, SR;Zill, OA;Sikora, M;Mokhtari, R;Abdueva, D;Nagy, RJ;Lee, CE;Kiedrowski, LA;Paweletz, CP;Eltoukhy, H;Lanman, RB;Chudova, DI;Talasaz, A; | Validation of a Plasma-Based Comprehensive Cancer Genotyping Assay Utilizing Orthogonal Tissue- and Plasma-Based Methodologies | Clinical cancer research : an official journal of the American Association for Cancer Research | 29691297 | Contrived analytic samples were generated using similarly prepared cfDNA from healthy donors (AllCells, Inc.) and cfDNA isolated as above from the culture supernatant of model cell Save | Full Paper |
2018 | L'Italien, L;Orozco, O;Abrams, T;Cantagallo, L;Connor, A;Desai, J;Ebersbach, H;Gelderblom, H;Hoffmaster, K;Lees, E;Maacke, H;Schleyer, S;Skegro, D;Lee-Hoeflich, ST; | Mechanistic Insights of an Immunological Adverse Event Induced by an Anti-KIT Antibody Drug Conjugate and Mitigation Strategies | Clinical cancer research : an official journal of the American Association for Cancer Research | 29615457 | Human peripheral blood cell-derived mast cells (PBC-MCs) were generated from mobilized peripheral blood CD34 + cells isolated from healthy donors (AllCells #mPB017F). Mast cells Save | Full Paper |
2018 | Cromer, MK;Vaidyanathan, S;Ryan, DE;Curry, B;Lucas, AB;Camarena, J;Kaushik, M;Hay, SR;Martin, RM;Steinfeld, I;Bak, RO;Dever, DP;Hendel, A;Bruhn, L;Porteus, MH; | Global Transcriptional Response to CRISPR/Cas9-AAV6-Based Genome Editing in CD34+ Hematopoietic Stem and Progenitor Cells | Molecular therapy : the journal of the American Society of Gene Therapy | 30005866 | CD34+ HSPCs used for gene expression microarrays were obtained as frozen stock derived from mobilized peripheral blood thawed according to manufacturer’s instructions (AllCells). For follow-up experiments, CD34+ HSPCs were obtained fresh from cord bl | Full Paper |
2018 | Ligtenberg, MA;Pico de Coaña, Y;Shmushkovich, T;Yoshimoto, Y;Truxova, I;Yang, Y;Betancur-Boissel, M;Eliseev, AV;Wolfson, AD;Kiessling, R; | Self-Delivering RNAi Targeting PD-1 Improves Tumor-Specific T Cell Functionality for Adoptive Cell Therapy of Malignant Melanoma | Molecular therapy : the journal of the American Society of Gene Therapy | 29735366 | ation (Ficoll-Paque Plus; GE Healthcare). T cells were purified using the Pan T cell isolation kit (Miltenyi Biotec) following manufacturer’s instructions. Peripheral blood CD3+ Pan T cells from AllCells (PB009-1F) were used for sdRNA validation and | Full Paper |
2018 | Georgiadis, C;Preece, R;Nickolay, L;Etuk, A;Petrova, A;Ladon, D;Danyi, A;Humphryes-Kirilov, N;Ajetunmobi, A;Kim, D;Kim, JS;Qasim, W; | Long Terminal Repeat CRISPR-CAR-Coupled "Universal" T Cells Mediate Potent Anti-leukemic Effects | Molecular therapy : the journal of the American Society of Gene Therapy | 29605708 | CliniMACS Prodigy using tubing set TS520 (130-019-002, Miltenyi Biotec) and used cryopreserved leukapheresis harvests (Allcells, Alameda, USA) cultured in TexMACS good manufacturing practice (GMP) medium (170-076-307, Miltenyi Biotec) supplemented wi | Full Paper |
2018 | Heffner, GC;Bonner, M;Christiansen, L;Pierciey, FJ;Campbell, D;Smurnyy, Y;Zhang, W;Hamel, A;Shaw, S;Lewis, G;Goss, KA;Garijo, O;Torbett, BE;Horton, H;Finer, MH;Gregory, PD;Veres, G; | Prostaglandin E2 Increases Lentiviral Vector Transduction Efficiency of Adult Human Hematopoietic Stem and Progenitor Cells | Molecular therapy : the journal of the American Society of Gene Therapy | 29102562 | CD34-enriched mPB samples from healthy human donors were obtained from AllCells (Emeryville, CA, USA), Key Biologics (Memphis, TN, USA), and primary patients under Save;Methods Cell Culture Research-grade GFP-, globin-, and ALDP-containing LVVs wer | Full Paper |
2018 | Mokhtari, S;Colletti, E;Yin, W;Sanada, C;Lamar, Z;Simmons, PJ;Walker, S;Bishop, C;Atala, A;Zanjani, ED;Porada, CD;Almeida-Porada, G; | A human bone marrow mesodermal-derived cell population with hemogenic potential | Leukemia | 29467489 | al bone tissues were obtained from Advanced Bioscience Resources (Alameda, CA). Human adult bone marrow (BM) was obtained from healthy adult donors after informed consent, or from commercial sources (AllCells, LLC, Alameda, CA). Fetal and adult tissu | Full Paper |
2018 | Tan, YT;Ye, L;Xie, F;Beyer, AI;Muench, MO;Wang, J;Chen, Z;Liu, H;Chen, SJ;Kan, YW; | Respecifying human iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells with a single factor | Proceedings of the National Academy of Sciences of the United States of America | 29386396 | Frozen human PB mobilized CD34+ cells and PB mononuclear cells were purchased from AllCells (www.allcells.com). The protocols involving human iPSCs were approved by Human Research Committee and Human Gamete, Embryo and Stem Cells Research Committee ( | Full Paper |
2018 | Li, Y;Carpenito, C;Wang, G;Surguladze, D;Forest, A;Malabunga, M;Murphy, M;Zhang, Y;Sonyi, A;Chin, D;Burtrum, D;Inigo, I;Pennello, A;Shen, L;Malherbe, L;Chen, X;Hall, G;Haidar, JN;Ludwig, DL;Novosiadly, RD;Kalos, M; | Discovery and preclinical characterization of the antagonist anti-PD-L1 monoclonal antibody LY3300054 | Journal for immunotherapy of cancer | 29712568 | s analyzed using GraphPad Prism software [25]. Mixed leukocyte reaction (MLR) CD14+ monocytes were isolated from frozen human peripheral blood mononuclear cells (PBMC) obtained from a healthy donor (AllCells, Alameda, CA) with Human Monocyte Isolati | Full Paper |
2018 | Liu, Y;Jing, H;Kou, X;Chen, C;Liu, D;Jin, Y;Lu, L;Shi, S; | PD-1 is required to maintain stem cell properties in human dental pulp stem cells | Cell death and differentiation | 29472716 | Isolation and culture of human BMMSCs Human bone marrow aspirates from healthy human adult volunteers (20-35 years of age) were purchased from AllCells LLC. hBMMSCs were cultured as reported previously [40] | Full Paper |
2018 | Willingham, SB;Ho, PY;Hotson, A;Hill, C;Piccione, EC;Hsieh, J;Liu, L;Buggy, JJ;McCaffery, I;Miller, RA; | A2AR Antagonism with CPI-444 Induces Antitumor Responses and Augments Efficacy to Anti-PD-(L)1 and Anti-CTLA-4 in Preclinical Models | Cancer immunology research | 30131376 | PBMCs were purchased from AllCells and either used fresh or cryopreserved for future use. Fresh PBMCs were cultured at a density of 10 7 cells/mL, and cryopreserved cells were Save | Full Paper |
2018 | Siriwon, N;Kim, YJ;Siegler, E;Chen, X;Rohrs, JA;Liu, Y;Wang, P; | CAR-T Cells Surface-Engineered with Drug-Encapsulated Nanoparticles Can Ameliorate Intratumoral T-cell Hypofunction | Cancer immunology research | 29720380 | Frozen human peripheral blood mononuclear cells (PBMC) were obtained from AllCells. Thawed PBMCs were cultured in T-cell medium (TCM) containing X-VIVO 15 serum-free Save | Full Paper |
2018 | Oeller, M;Laner-Plamberger, S;Hochmann, S;Ketterl, N;Feichtner, M;Brachtl, G;Hochreiter, A;Scharler, C;Bieler, L;Romanelli, P;Couillard-Despres, S;Russe, E;Schallmoser, K;Strunk, D; | Selection of Tissue Factor-Deficient Cell Transplants as a Novel Strategy for Improving Hemocompatibility of Human Bone Marrow Stromal Cells | Theranostics | 29507631 | Three additional BM samples (10 mL each) were purchased via CellSystems from ALLCELLS (USA). The mean age of the male BM donors was 34 years (range 20-46) and of the Save | Full Paper |
2018 | de la Puente, P;Luderer, MJ;Federico, C;Jin, A;Gilson, RC;Egbulefu, C;Alhallak, K;Shah, S;Muz, B;Sun, J;King, J;Kohnen, D;Salama, NN;Achilefu, S;Vij, R;Azab, AK; | Enhancing proteasome-inhibitory activity and specificity of bortezomib by CD38 targeted nanoparticles in multiple myeloma | Journal of controlled release : official journal of the Controlled Release Society | 29196043 | Bone Marrow mononuclear cells (BM MNCs) from 5 different patient samples were purchased from Allcells (Alameda, CA). Peripheral blood mononuclear cells (PB MNCs) were Save | Full Paper |
2018 | Chen, Y;Mao, P;Snijders, AM;Wang, D; | Senescence chips for ultrahigh-throughput isolation and removal of senescent cells | Aging cell | 29336105 | Fresh human whole blood from healthy donors collected within 24 hr was purchased from AllCells Inc. (Alameda, CA). Human MSCs were purchased from Lonza (Lonza, Swiss). Save | Full Paper |
2018 | Charlesworth, CT;Camarena, J;Cromer, MK;Vaidyanathan, S;Bak, RO;Carte, JM;Potter, J;Dever, DP;Porteus, MH; | Priming Human Repopulating Hematopoietic Stem and Progenitor Cells for Cas9/sgRNA Gene Targeting | Molecular therapy. Nucleic acids | 30195800 | CD34 + HSPCs derived from mobilized peripheral blood were purchased from AllCells, and CD34 + HSPCs derived from cord blood were provided from donors under informed consent Save;ollowing the manufacturer’s instructions. Titers were measured after b | Full Paper |
2018 | Vaidyanathan, S;Azizian, KT;Haque, AKMA;Henderson, JM;Hendel, A;Shore, S;Antony, JS;Hogrefe, RI;Kormann, MSD;Porteus, MH;McCaffrey, AP; | Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification | Molecular therapy. Nucleic acids | 30195789 | CD34 + HSPCs derived from mobilized peripheral blood donated by male donors were purchased from AllCells (Alemeda, CA). Cells were thawed according to the manufacturer's Save;tection System luminometer (Promega, E8032) with a 10-s integration per w | Full Paper |
2018 | Yoshinaga, T;Seki, T;Miki, S;Miyamoto, T;Suyama-Kagitani, A;Kawauchi-Miki, S;Kobayashi, M;Sato, A;Stewart, E;Underwood, M;Fujiwara, T; | Novel secondary mutations C56S and G149A confer resistance to HIV-1 integrase strand transfer inhibitors | Antiviral research | 29410019 | kanamycin sulfate. Peripheral blood mononuclear cells (PBMCs; AllCells, Alameda, CA) were cultured in RPMI 1640 medium supplemented with 10% FBS, 60 μg/mL of kanamycin sulfate, and 10 μg/mL of phytohemagglutinin (PHA)-M (Roche, Indianapolis, IN) for | Full Paper |
2018 | Li, S;Hong, Z;Wang, Z;Li, F;Mei, J;Huang, L;Lou, X;Zhao, S;Song, L;Chen, W;Wang, Q;Liu, H;Cai, Y;Yu, H;Xu, H;Zeng, G;Wang, Q;Zhu, J;Liu, X;Tan, N;Wang, C; | The Cyclopeptide Astin C Specifically Inhibits the Innate Immune CDN Sensor STING | Cell reports | 30566866 | hPBMCs and IMR-90 (female) cells were purchased from AllCells and Cobioer Bioscience, respectively. The gender information of hPBMCs is not available from the supplier. The Save | Full Paper |
2018 | Osorio, FG;Rosendahl Huber, A;Oka, R;Verheul, M;Patel, SH;Hasaart, K;de la Fonteijne, L;Varela, I;Camargo, FD;van Boxtel, R; | Somatic Mutations Reveal Lineage Relationships and Age-Related Mutagenesis in Human Hematopoiesis | Cell reports | 30485801 | nd Cat# 302005; RRID:AB_314205 CD11c-FITC BioLegend Cat# 301603; RRID:AB_314173 ------------------------- Biological Samples ------------------------- Whole bone marrow samples A,C,D,E AllCells ABM001 Whole bone marrow sample B B | Full Paper |
2018 | Zhu, YP;Padgett, L;Dinh, HQ;Marcovecchio, P;Blatchley, A;Wu, R;Ehinger, E;Kim, C;Mikulski, Z;Seumois, G;Madrigal, A;Vijayanand, P;Hedrick, CC; | Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone Marrow | Cell reports | 30157427 | Fresh BM samples of anonymous healthy adult donors were obtained from AllCells, Inc. (Alameda, CA). The cells were stained for either flow cytometry or FACS-sorting following protocols described in the Flow Cytometry and Cell Sorting section | Full Paper |
2018 | Schaer, DA;Beckmann, RP;Dempsey, JA;Huber, L;Forest, A;Amaladas, N;Li, Y;Wang, YC;Rasmussen, ER;Chin, D;Capen, A;Carpenito, C;Staschke, KA;Chung, LA;Litchfield, LM;Merzoug, FF;Gong, X;Iversen, PW;Buchanan, S;de Dios, A;Novosiadly, RD;Kalos, M; | The CDK4/6 Inhibitor Abemaciclib Induces a T Cell Inflamed Tumor Microenvironment and Enhances the Efficacy of PD-L1 Checkpoint Blockade | Cell reports | 29539425 | Breast cancer cell lines MCF-7 and MDA-MB-468 (American Type Culture Collection [ATCC]) were treated with DMSO and abemaciclib (500 nM) for 8 days. RNA was isolated as described above. Jurkat T cells (ATCC) or primary human T cells (AllCells) were st | Full Paper |
2018 | Shi, J;Zhang, M;Zhang, L;Deng, H; | Epigallocatechin-3-gallate attenuates microcystin-LR-induced apoptosis in human umbilical vein endothelial cells through activation of the NRF2/HO-1 pathway | Environmental pollution (Barking, Essex : 1987) | 29679944 | HUVECs were obtained from Allcells Technology (Shanghai, China) and seeded in complete medium (HUVEC-004, AllCells Technology) in a humidified atmosphere of 5% CO 2 at 37 Save | Full Paper |
2018 | Wienert, B;Shin, J;Zelin, E;Pestal, K;Corn, JE; | In vitro-transcribed guide RNAs trigger an innate immune response via the RIG-I pathway | PLoS biology | 30011268 | HSPCs from mobilized peripheral blood (Allcells) were thawed and cultured in StemSpan SFEM medium (StemCell Technologies) supplemented with StemSpan CC110 cocktail ( Save | Full Paper |
2018 | Yang, W;Sun, Z;Hua, C;Wang, Q;Xu, W;Deng, Q;Pan, Y;Lu, L;Jiang, S; | Chidamide, a histone deacetylase inhibitor-based anticancer drug, effectively reactivates latent HIV-1 provirus | Microbes and infection | 29126877 | J-Lat A2 cells [25] (obtained from NIH AIDS Reagent Program), J-Lat C11 cells [26], PBMCs (Allcells), ACH-2 cells [27] and U1 cells [28] were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS) and 1% Pen/strep in a 37 °C incubator contain | Full Paper |
2018 | Ito, R;Maruoka, S;Soda, K;Katano, I;Kawai, K;Yagoto, M;Hanazawa, A;Takahashi, T;Ogura, T;Goto, M;Takahashi, R;Toyoshima, S;Okayama, Y;Izuhara, K;Gon, Y;Hashimoto, S;Ito, M;Nunomura, S; | A humanized mouse model to study asthmatic airway inflammation via the human IL-33/IL-13 axis | JCI insight | 30385714 | Commercially available human cord blood-derived CD34 + cells (AllCells LLC or Lonza) were used in this study. The cells were prepared according to the manufacturer's instructions. Save | Full Paper |
2018 | Antebi, B;Rodriguez, LA;Walker, KP;Asher, AM;Kamucheka, RM;Alvarado, L;Mohammadipoor, A;Cancio, LC; | Short-term physiological hypoxia potentiates the therapeutic function of mesenchymal stem cells | Stem cell research & therapy | 30305185 | arrow MSCs (pMSCs) were isolated from mononuclear cells (MNCs) obtained from bone marrow aspirates, as previously described [39]. Human bone marrow MSCs (hMSCs) were isolated from MNCs purchased from AllCells LLC (Emeryville, CA, USA). MNCs were cult | Full Paper |
2018 | Senyuk, V;Patel, P;Mahmud, N;Rondelli, D; | Blockade of TNFα to Improve Human CD34+ Cell Repopulating Activity in Allogeneic Stem Cell Transplantation | Frontiers in immunology | 30723481 | Healthy donor G-CSF mobilized peripheral blood stem cells (PBSC) from AllCells (Alameda, CA) and PB cells from healthy volunteers were utilized in this study. Mononuclear cells ( Save | Full Paper |
2018 | Cui, C;Su, M;Lin, Y;Lai, L; | A CD300c-Fc Fusion Protein Inhibits T Cell Immunity | Frontiers in immunology | 30498497 | peripheral blood CD3 + Pan T Cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan-T labeling system were purchased from ALLCELLS, Save | Full Paper |
2018 | Cobbold, SP;Adams, E;Howie, D;Waldmann, H; | CD4+ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development | Frontiers in immunology | 29967616 | t CV (%) is indicated. In all experiments, P values of | Full Paper |
2018 | Burton, OT;Epp, A;Fanny, ME;Miller, SJ;Stranks, AJ;Teague, JE;Clark, RA;van de Rijn, M;Oettgen, HC; | Tissue-Specific Expression of the Low-Affinity IgG Receptor, FcγRIIb, on Human Mast Cells | Frontiers in immunology | 29928276 | genic for membrane-bound human stem cell factor (SCF) [NOD.Cg-PrkdcscidIl2rgtm1Wjl Tg(PGK1-KITLG*220)441Daw/SzJ] were engrafted with 5 × 104 CD34+ hematopoietic stem cells (HSC) from cord blood (AllCells) for 16-24 weeks. Wild-type BALB/c, C57BL/6J, | Full Paper |
2018 | White, CH;Beliakova-Bethell, N;Lada, SM;Breen, MS;Hurst, TP;Spina, CA;Richman, DD;Frater, J;Magiorkinis, G;Woelk, CH; | Transcriptional Modulation of Human Endogenous Retroviruses in Primary CD4+ T Cells Following Vorinostat Treatment | Frontiers in immunology | 29706951 | rials and Methods Isolation of Primary CD4+ T Cells For subsequent total RNA-Seq analysis, cryopreserved primary CD4+ T cells that were viably frozen were obtained from four different healthy donors (AllCells, Inc., Emeryville, CA, USA) and thawed in | Full Paper |
2018 | Bhaskaran, N;Liu, Z;Saravanamuthu, SS;Yan, C;Hu, Y;Dong, L;Zelenka, P;Zheng, L;Bletsos, V;Harris, R;Harrington, B;Weinberg, A;Thiele, CJ;Ye, F;Pandiyan, P; | Identification of Casz1 as a Regulatory Protein Controlling T Helper Cell Differentiation, Inflammation, and Immunity | Frontiers in immunology | 29467767 | done at NIAID, NIH under an approved protocol, and in compliance with the NIAID Institutional Animal Care and Use Committee’s guidelines. Human cells were obtained from commercially available PBMC (AllCells). Reagents and Antibodies Purified or fluo | Full Paper |
2018 | Hanazawa, A;Ito, R;Katano, I;Kawai, K;Goto, M;Suemizu, H;Kawakami, Y;Ito, M;Takahashi, T; | Generation of Human Immunosuppressive Myeloid Cell Populations in Human Interleukin-6 Transgenic NOG Mice | Frontiers in immunology | 29456539 | ined in the Central Institute for Experimental Animals (CIEA) under specific pathogen-free conditions. Transplantation of Human HSCs Human umbilical-cord-blood-derived CD34+ HSCs were purchased from Allcells (Alameda, CA, USA). For transplantation, | Full Paper |
2018 | Seto, AG;Beatty, X;Lynch, JM;Hermreck, M;Tetzlaff, M;Duvic, M;Jackson, AL; | Cobomarsen, an oligonucleotide inhibitor of miR-155, co-ordinately regulates multiple survival pathways to reduce cellular proliferation and survival in cutaneous T-cell lymphoma | British journal of haematology | 30125933 | miR‐155, a microRNA associated with poor prognosis in lymphoma and leukaemia, has been implicated in the progression of mycosis fungoides ( MF ), the most common form of Save | Full Paper |
2018 | Makar, T;Nimmagadda, VK;Guda, PR;Hampton, B;Huang, W;Kane, MA;Fishman, PS;Pessac, B;Bever, CT;Trisler, D; | A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions | Stem cell research & therapy | 29720239 | Human buffy coat cells and CD34 + cells from peripheral blood were purchased from ALLCELLS (Alameda, CA, USA). Human buffy coat cells were obtained by differential Save | Full Paper |
2018 | Fujisawa, K;Hara, K;Takami, T;Okada, S;Matsumoto, T;Yamamoto, N;Sakaida, I; | Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cells | Stem cell research & therapy | 29625581 | MSCs were derived from bone marrow cells (AllCells, Alameda, CA,USA) and were used at passage (P)4. Mononuclear cells (MNCs) were purchased from Lonza, Tokyo, Japan. Flow Save | Full Paper |
2018 | Thekkeparambil Chandrabose, S;Sriram, S;Subramanian, S;Cheng, S;Ong, WK;Rozen, S;Kasim, NHA;Sugii, S; | Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming | Stem cell research & therapy | 29559008 | The list of commercial (Lonza and Allcells) and patient-derived dental cells used in this manuscript can be found in Additional file 1: Table S1. DPSCs were grown in vitro in Poietics Save | Full Paper |
2018 | Johnson, HWB;Lowe, E;Anderl, JL;Fan, A;Muchamuel, T;Bowers, S;Moebius, DC;Kirk, C;McMinn, DL; | Required Immunoproteasome Subunit Inhibition Profile for Anti-Inflammatory Efficacy and Clinical Candidate KZR-616 ((2 S,3 R)- N-(( S)-3-(Cyclopent-1-en-1-yl)-1-(( R)-2-methyloxiran-2-yl)-1-oxopropan-2-yl)-3-hydroxy-3-(4-methoxyphenyl)-2-(( S)-2-(2-morpholinoacetamido)propanamido)propenamide) | Journal of medicinal chemistry | 30380863 | Cryopreserved peripheral blood mononuclear cells (PBMCs) from normal healthy volunteers were purchased from AllCells (Alameda, CA) and cultured in RPMI 1640 media (with 300 mg/L l-glutamine) supplemented with 5% heat-inactivated FBS, 100 IU penicilli | Full Paper |
2018 | Vazquez, ML;Kaila, N;Strohbach, JW;Trzupek, JD;Brown, MF;Flanagan, ME;Mitton-Fry, MJ;Johnson, TA;TenBrink, RE;Arnold, EP;Basak, A;Heasley, SE;Kwon, S;Langille, J;Parikh, MD;Griffin, SH;Casavant, JM;Duclos, BA;Fenwick, AE;Harris, TM;Han, S;Caspers, N;Dowty, ME;Yang, X;Banker, ME;Hegen, M;Symanowicz, PT;Li, L;Wang, L;Lin, TH;Jussif, J;Clark, JD;Telliez, JB;Robinson, RP;Unwalla, R; | Identification of N-{cis-3-[Methyl(7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino]cyclobutyl}propane-1-sulfonamide (PF-04965842): A Selective JAK1 Clinical Candidate for the Treatment of Autoimmune Diseases | Journal of medicinal chemistry | 29298069 | Cryopreserved human bone marrow CD34+ cells (cat. no. ABM022F) were purchased from Allcells. Frozen bone marrow CD34+ cells were thawed, washed once with IMEM, resuspended in Stem Span medium (cat. no. 09600; STEMCELL Technologies, Vancouver, Canada) | Full Paper |
2018 | Xu, H;Liao, C;Zuo, P;Liu, Z;Ye, BC; | Magnetic-Based Microfluidic Device for On-Chip Isolation and Detection of Tumor-Derived Exosomes | Analytical chemistry | 30234974 | CD63 antibody (anti-CD63) from Biolegend (USA), biotin-labeled mouse antihuman CD9 antibody (anti-CD9) from Allcells Biotech Co., Ltd. (Shanghai, China), and HRP-conjugated antimouse IgG from QF Biosciences Co., Ltd. (Shanghai, China). MagCapture Exo | Full Paper |
2018 | Xu, H;Liao, C;Liu, Y;Ye, BC;Liu, B; | Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living Cells | Analytical chemistry | 29508999 | Human umbilical vein endothelial cells (HUVECs) (ALLCELLS, Shanghai) were cultured in a humidified incubator (95% air with 5% CO2) at 37 °C with a complete medium designed for optimal growth of endothelial cells in vitro. A 6 mm thick PDMS containing | Full Paper |
2018 | Yasinska, IM;Gonçalves Silva, I;Sakhnevych, SS;Ruegg, L;Hussain, R;Siligardi, G;Fiedler, W;Wellbrock, J;Bardelli, M;Varani, L;Raap, U;Berger, S;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV; | High mobility group box 1 (HMGB1) acts as an "alarmin" to promote acute myeloid leukaemia progression | Oncoimmunology | 29872582 | lemented with 10% foetal bovine serum, penicillin (50 IU/ml) and streptomycin sulphate (50 µg/ml). Primary human AML mononuclear blasts (AML-PB001 F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordanc | Full Paper |
2018 | Lam, J;Bellayr, IH;Marklein, RA;Bauer, SR;Puri, RK;Sung, KE; | Functional Profiling of Chondrogenically Induced Multipotent Stromal Cell Aggregates Reveals Transcriptomic and Emergent Morphological Phenotypes Predictive of Differentiation Capacity | Stem cells translational medicine | 30084545 | rived MSCs were obtained from eight different donors purchased from RoosterBio (RB9, RB12, RB14, RB16; Frederick, MD) at passage 1 (P1), Lonza (8F560, 167696; Walkersville, MD) at passage 2 (P2), and AllCells (PCBM1632, PCBM1641; Alameda, CA) at P2 ( | Full Paper |
2018 | George, MJ;Prabhakara, K;Toledano-Furman, NE;Wang, YW;Gill, BS;Wade, CE;Olson, SD;Cox, CS; | Clinical Cellular Therapeutics Accelerate Clot Formation | Stem cells translational medicine | 30070065 | Bone marrow derived MSCs (BM MSCs) were isolated from commercially available fresh human bone marrow aspirates (AllCells, Emeryville, CA) or obtained from fresh bone marrow Save | Full Paper |
2018 | Marcellino, BK;Hoffman, R;Tripodi, J;Lu, M;Kosiorek, H;Mascarenhas, J;Rampal, RK;Dueck, A;Najfeld, V; | Advanced forms of MPNs are accompanied by chromosomal abnormalities that lead to dysregulation of TP53 | Blood advances | 30563882 | Normal bone marrow MNCs were obtained from AllCells (Alameda, CA). RNA was extracted from MNCs, using RNeasy mini kit (Fisher Scientific, Waltham, MA), and was reverse Save | Full Paper |
2018 | Wei, Y;Zheng, H;Bao, N;Jiang, S;Bueso-Ramos, CE;Khoury, J;Class, C;Lu, Y;Lin, K;Yang, H;Ganan-Gomez, I;Starczynowski, DT;Do, KA;Colla, S;Garcia-Manero, G; | KDM6B overexpression activates innate immune signaling and impairs hematopoiesis in mice | Blood advances | 30275007 | BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). CD34 + cells were isolated using MicroBead Kit (Miltenyi Biotec). For colony formation, BM CD34 + Save | Full Paper |
2018 | Witt, RG;Kreger, EM;Buckman, LB;Moradi, PW;Ho, PT;Derderian, SC;Tsai, P;Baker, C;Schramm, N;Cleary, R;Garcia, JV;MacKenzie, TC; | Systemic multilineage engraftment in mice after in utero transplantation with human hematopoietic stem cells | Blood advances | 29344586 | Human cord blood CD34 + cells were either purchased from AllCells (Alameda, CA) or purified from cord blood collected at time of delivery from normal-term infants. CD34 + cells were Save | Full Paper |
2018 | Murray, C;Miwa, H;Dhar, M;Park, DE;Pao, E;Martinez, J;Kaanumale, S;Loghin, E;Graf, J;Raddassi, K;Kwok, WW;Hafler, D;Puleo, C;Di Carlo, D; | Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratcheting | Lab on a chip | 30039125 | Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) were purchased from ALLCELLs (Alameda, CA, USA; Cat No. PB003F) and stored at −180°C in vapor phase liquid nitrogen until ready for experimental use. Cells were thawed by placing cryovial in 37 | Full Paper |
2018 | Romero-Masters, JC;Ohashi, M;Djavadian, R;Eichelberg, MR;Hayes, M;Bristol, JA;Ma, S;Ranheim, EA;Gumperz, J;Johannsen, EC;Kenney, SC; | An EBNA3C-deleted Epstein-Barr virus (EBV) mutant causes B-cell lymphomas with delayed onset in a cord blood-humanized mouse model | PLoS pathogens | 30125329 | Commercially available CD34-depleted human cord blood mononuclear cells (AllCells) were infected with 5000 Green Raji Units of wild-type or mutant EBV strains in vitro for 1 ½ hours Save | Full Paper |
2018 | Miller, AZ;Satchie, A;Tannenbaum, AP;Nihal, A;Thomson, JA;Vereide, DT; | Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal Transition | Stem cell reports | 29320761 | scribed in Figure 5 as a means to transiently label transfected cells. Sources of Primary Cells Frozen primary CD34+ cells purified from umbilical cord blood or adult bone marrow were obtained from AllCells (for cord blood, products CB005F and CB009 | Full Paper |
2018 | Tsai, HC;Velichko, S;Lee, S;Wu, R; | Cholera toxin enhances interleukin-17A production in both CD4+ and CD8+ cells via a cAMP/protein kinase A-mediated interleukin-17A promoter activation | Immunology | 29377102 | Peripheral blood mononuclear cells were purchased from Allcells, LLC (Alameda, CA) or isolated from the leukopak filters from the Delta Blood Bank in Stockton, CA. Total CD4 + T Save | Full Paper |
2018 | Yang, R;Liu, Y;Yu, T;Liu, D;Shi, S;Zhou, Y;Zhou, Y; | Hydrogen sulfide maintains dental pulp stem cell function via TRPV1-mediated calcium influx | Cell death discovery | 30062050 | 00 μg/ml streptomycin and passaged, as previously reported27. Human bone marrow MSCs BMMSC culture Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells (Emeryville, CA, USA). Human bone marrow proge | Full Paper |
2018 | Zhou, Z;Shao, T;Qin, M;Miao, X;Chang, Y;Sheng, W;Wu, F;Yu, Y; | The effects of autophagy on vascular endothelial cells induced by airborne PM2.5 | Journal of environmental sciences (China) | 29628085 | In this study, human umbilical vein endothelial cells (HUVECs) were obtained from Allcells, China. The cells were routinely maintained in an HUVEC complete culture medium (Allcells, Save | Full Paper |
2018 | Figueroa, I;Leipold, D;Leong, S;Zheng, B;Triguero-Carrasco, M;Fourie-O'Donohue, A;Kozak, KR;Xu, K;Schutten, M;Wang, H;Polson, AG;Kamath, AV; | Prediction of non-linear pharmacokinetics in humans of an antibody-drug conjugate (ADC) when evaluation of higher doses in animals is limited by tolerability: Case study with an anti-CD33 ADC | mAbs | 29757698 | 61 Samples included normal human whole blood donors (n = 6 from Genentech, Inc. South San Francisco, CA; for phenotyping lymphocyte and myeloid subsets), normal human bone marrow donors (n = 5 from ALLCells, Alameda, CA; for phenotyping hematopoietic | Full Paper |
2018 | Jiang, N;Wang, Y;Yin, Y;Wei, R;Ying, G;Li, B;Qiu, T;van Rijn, P;Tian, G;Yan, Q;Dai, H;Busscher, H;Li, S;Yetisen, A;Yang, X; | bFGF and Poly-RGD Cooperatively Establish Biointerface for Stem Cell Adhesion, Proliferation, and Differentiation | Advanced Materials Interfaces | hBMMSCs Culture: Freshly isolated human bone marrow mononuclear cells obtained from a 23-year-old donor were purchased from ALLCELLS (Emeryville, CA, US), and cultured with α-minimum essential media (MEM)/15% FBS/glutamine (2 mmol L−1)/penicillin (10 | Full Paper | |
2018 | Hoff, FW;Hu, CW;Qiu, Y;Ligeralde, A;Yoo, SY;Mahmud, H;de Bont, ESJM;Qutub, AA;Horton, TM;Kornblau, SM; | Recognition of Recurrent Protein Expression Patterns in Pediatric Acute Myeloid Leukemia Identified New Therapeutic Targets | Molecular cancer research : MCR | 29669821 | patients (57 pre-B ALL and 16 T-ALL, patient demographics are presented in Supplemental Table S2), 10 cryopreserved normal BM CD34+ (AllCells, Almeda CA), and 127 leukemic cell line samples. The methods and validation of the technique, as well as the | Full Paper |
2018 | Bates, AM;Fischer, CL;Abhyankar, VP;Johnson, GK;Guthmiller, JM;Progulske-Fox, A;Brogden, KA; | Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-B | International journal of molecular sciences | 30544510 | ls, GE keratinocytes, and T-cells were chosen for this experiment due to their presence in the periodontium and their innate immune functions. Human monocyte-derived immature myeloid dendritic cells (AllCells, Alameda, CA, USA) were thawed, washed wi | Full Paper |
2018 | Chen, Q;Smith, S;Fong, T;Shirazian, A;Lee, J;Chapman, L;Tong, J;Vaz, W; | Practical considerations for sourcing clinical-grade human tissue to support development and production of emerging commercial cellular therapies | Cytotherapy | Q. Chen, S. Smith, T. Fong, A. Shirazian, J. Lee, L. Chapman, J. Tong, W. Vaz Commercial, AllCells, LLC, Alameda, California, United States The rapid clinical advancement and commercial manufacturing of cellular therapeutics pose numerous industrial | Full Paper | |
2018 | Okragly, AJ;Morin, SM;DeRosa, D;Martin, AP;Johnson, KW;Johnson, MP;Benschop, RJ; | Human mast cells release the migraine-inducing factor pituitary adenylate cyclase-activating polypeptide (PACAP) | Cephalalgia : an international journal of headache | 29103295 | Human umbilical cord blood-derived mast cells were produced by in vitro differentiation of positively selected cord blood CD34+ progenitor cells (Allcells) as previously described (37,38). Cells were cultured in Serum Free Expansion Media (SFEM Cat. | Full Paper |
2018 | Oberst, MD;Augé, C;Morris, C;Kentner, S;Mulgrew, K;McGlinchey, K;Hair, J;Hanabuchi, S;Du, Q;Damschroder, M;Feng, H;Eck, S;Buss, N;de Haan, L;Pierce, AJ;Park, H;Sylwester, A;Axthelm, MK;Picker, L;Morris, NP;Weinberg, A;Hammond, SA; | Potent Immune Modulation by MEDI6383, an Engineered Human OX40 Ligand IgG4P Fc Fusion Protein | Molecular cancer therapeutics | 29545330 | Ligation of OX40 (CD134, TNFRSF4) on activated T cells by its natural ligand (OX40L, CD252, TNFSF4) enhances cellular survival, proliferation, and effector functions such as cytokine Save | Full Paper |
2018 | Fujisawa, K;Takami, T;Okada, S;Hara, K;Matsumoto, T;Yamamoto, N;Yamasaki, T;Sakaida, I; | Analysis of Metabolomic Changes in Mesenchymal Stem Cells on Treatment with Desferrioxamine as a Hypoxia Mimetic Compared with Hypoxic Conditions | Stem cells (Dayton, Ohio) | 29577517 | Cells and Cell Culturing BMSCs were obtained from bone marrow cells (age: 34, male) purchased from AllCells (Alameda, CA), and BMSCs were proliferated using passage 4 and passage 16. BMSCs were confirmed to be CD45 (-), CD11b (-), CD90 (+), CD73 (+), | Full Paper |
2018 | Fujii, S;Miura, Y;Fujishiro, A;Shindo, T;Shimazu, Y;Hirai, H;Tahara, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T; | Graft-Versus-Host Disease Amelioration by Human Bone Marrow Mesenchymal Stromal/Stem Cell-Derived Extracellular Vesicles Is Associated with Peripheral Preservation of Naive T Cell Populations | Stem cells (Dayton, Ohio) | 29239062 | Human MSCs were isolated from BM samples purchased from AllCells (Alameda, CA) using our previously published method [21, 22]. BM samples were obtained from three different Save | Full Paper |
2018 | Harting, MT;Srivastava, AK;Zhaorigetu, S;Bair, H;Prabhakara, KS;Toledano Furman, NE;Vykoukal, JV;Ruppert, KA;Cox, CS;Olson, SD; | Inflammation-Stimulated Mesenchymal Stromal Cell-Derived Extracellular Vesicles Attenuate Inflammation | Stem cells (Dayton, Ohio) | 29076623 | Human mesenchymal stromal cells (hMSCs) were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using Save | Full Paper |
2018 | Meader, E;Barta, T;Melguizo-Sanchis, D;Tilgner, K;Montaner, D;El-Harouni, AA;Armstrong, L;Lako, M; | Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs | Stem cells (Dayton, Ohio) | 29047185 | and TaqMan MicroRNA Assays were used for the qRT‐PCR (all from Thermo Fisher Scientific) RNU44 and RNU48 were chosen as internal controls. CD34+ human adult bone marrow and CD34+ cord blood cells (AllCells, Alameda, CA, USA) were used as positive con | Full Paper |
2018 | Wang, J;Li, T;Feng, J;Li, L;Wang, R;Cheng, H;Yuan, Y; | Kaempferol protects against gamma radiation-induced mortality and damage via inhibiting oxidative stress and modulating apoptotic molecules in vivo and vitro | Environmental toxicology and pharmacology | 29705372 | To investigate the potential protective effect of kaempferol, a representative flavonoid, against radiation induced mortality and injury in vivo and vitro.C57BL/6 male mice and human Save | Full Paper |
2018 | Garbuzova-Davis, S;Haller, E;Navarro, S;Besong, TE;Boccio, KJ;Hailu, S;Khatib, M;Sanberg, PR;Appel, SH;Borlongan, CV; | Transplantation of human bone marrow stem cells into symptomatic ALS mice enhances structural and functional blood-spinal cord barrier repair | Experimental neurology | 30172620 | Accumulating evidence shows alterations in the blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) in ALS patients and in animal models of disease, mainly by endothelial Save | Full Paper |
2018 | Huang, L;Zhang, X;Ma, X;Zhang, D;Li, D;Feng, J;Pan, X;Lü, J;Wang, X;Liu, X; | Berberine alleviates endothelial glycocalyx degradation and promotes glycocalyx restoration in LPS-induced ARDS | International immunopharmacology | 30308440 | (Shanghai, China) and grown to confluence in 25 cm 2 flasks supplemented with complete culture medium, which was provided by AllCells. The cells were incubated under 95% Save | Full Paper |
2018 | Romero, Z;DeWitt, M;Walters, MC; | Promise of gene therapy to treat sickle cell disease | Expert opinion on biological therapy | 30324810 | MC Walters is the Medical Director of AllCells, Inc and ViaCord Processing Lab; Consultant for Sangamo Therapeutics, INc, bluebird bio, Inc., and Bioverativ, Inc. The authors have no other relevant affiliations or financial involvement with any organ | Full Paper |
2018 | Hu, Y;Yao, X;Liu, Q;Wang, Y;Liu, R;Cui, S;Ding, J; | Left-Right Symmetry or Asymmetry of Cells on Stripe-Like Micropatterned Material Surfaces | Chinese Journal of Chemistry | rMSCs were bought from Allcells Biological Technology, Shanghai. hMSCs were from Cyagen Biosciences, Suzhou. HFFs and HUVECs were products of Bogoo Biological Technology, Shanghai. The cells of rMSCs were incubated in minimum essential medium α (MEM | Full Paper | |
2018 | Yuan, X;Han, L;Fu, P;Zeng, H;Lv, C;Chang, W;Runyon, RS;Ishii, M;Han, L;Liu, K;Fan, T;Zhang, W;Liu, R; | Cinnamaldehyde accelerates wound healing by promoting angiogenesis via up-regulation of PI3K and MAPK signaling pathways | Laboratory investigation; a journal of technical methods and pathology | 29463877 | Cell culture and animal maintenance Primary HUVECs were purchased from AllCells (Emeryville, CA, USA). With the exception of the homocysteine-rescue tube assay, each batch of HUVECs was examined using flow cytometry and was shown to be 99% CD31 posit | Full Paper |
2018 | Huang, T;Mathieu, M;Lee, S;Wang, X;Kee, YS;Bevers, JJ;Ciferri, C;Estavez, A;Wong, M;Chiang, NY;Nakamura, G;Brezski, RJ; | Molecular characterization of human anti-hinge antibodies derived from single-cell cloning of normal human B cells | The Journal of biological chemistry | 29191832 | PBMCs were isolated from a Leuko Pak (AllCells) using Ficoll-Paque gradient fractionation (GE Healthcare). PBMCs were then frozen in 90% fetal bovine serum (FBS) supplemented with 10% dimethyl sulfoxide (DMSO) and stored in liquid nitrogen until thaw | Full Paper |
2018 | Smith, AJ;Graves, B;Child, R;Rice, PJ;Ma, Z;Lowman, DW;Ensley, HE;Ryter, KT;Evans, JT;Williams, DL; | Immunoregulatory Activity of the Natural Product Laminarin Varies Widely as a Result of Its Physical Properties | Journal of immunology (Baltimore, Md. : 1950) | 29246954 | PBMCs obtained via leukapheresis were purchased from AllCells (Alameda, CA) and cryopreserved in 50% RPMI 1640 with 40% FBS/10% DMSO, after extensive washing in 1× PBS. Save | Full Paper |
2018 | Yang, C;Huan, Z;Wang, X;Wu, C;Chang, J; | 3D Printed Fe Scaffolds with HA Nanocoating for Bone Regeneration | ACS biomaterials science & engineering | 33418749 | initial density of 1× 104 rabbit bone marrow mesenchymal stem cells (rBMSCs) (Allcells, Silicon Valley, CA, USA) in 48-well tissue culture plates and then incubated at 37 °C wiht 5% CO2. After 24 h, the cell culture medium was changed for the first t | Full Paper |
2018 | Piao, XM;Gao, F;Zhu, JX;Wang, LJ;Zhao, X;Li, X;Sheng, MM;Zhang, Y; | Cucurbitacin B inhibits tumor angiogenesis by triggering the mitochondrial signaling pathway in endothelial cells | International journal of molecular medicine | 29717773 | HUVECs and HepG2 cells were obtained from Allcells, LLC (Shanghai, China). CuB (purity 99%) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China). DMEM/F-12, fetal bovine serum (FBS), was from Gibco (Carlsbad, CA, USA). EGM-2 complete medi | Full Paper |
2018 | Zhu, H;Yu, B;Yang, L; | Exogenous and Endogenous Stem Cells for Skeletal Regeneration | Stem cells international | 30581472 | HUVECs were purchased from AllCells, Ltd. (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth factors [19]. To investigate the Save | Full Paper |
2018 | Zhang, M;Yu, W;Niibe, K;Zhang, W;Egusa, H;Tang, T;Jiang, X; | The Effects of Platelet-Derived Growth Factor-BB on Bone Marrow Stromal Cell-Mediated Vascularized Bone Regeneration | Stem cells international | 30515221 | HUVECs were purchased from AllCells, Ltd. (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth factors [19]. To investigate the Save | Full Paper |
2018 | Kwak, J;Choi, SJ;Oh, W;Yang, YS;Jeon, HB;Jeon, ES; | Cobalt Chloride Enhances the Anti-Inflammatory Potency of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells through the ERK-HIF-1α-MicroRNA-146a-Mediated Signaling Pathway | Stem cells international | 30254683 | 103 cells/well) were seeded and maintained at 37°C in a humidified incubator for 2-4 hr and then cocultured with responder peripheral blood mononuclear cells (PBMCs; 1 × 105 cells/well; AllCells, Boston, MA, USA) and stimulator PBMCs (1 × 105 cells/ | Full Paper |
2018 | Chen, B;Ni, Y;Liu, J;Zhang, Y;Yan, F; | Bone Marrow-Derived Mesenchymal Stem Cells Exert Diverse Effects on Different Macrophage Subsets | Stem cells international | 30140291 | y directed activation. Western blot was used to detect the expression of iNOS and Arg-1 in the cells to further verify the M1 and M2 polarization. 2.5. Preparation of MSC-CM BMMSC was purchased from AllCells, LLC (Emeryville, CA), and MSCs at passag | Full Paper |
2018 | Jiang, J;Xu, K;Wang, L;Xin, W;Zhao, G;Huang, M;Li, S;Luan, X;Fang, J; | Pharmacology study of a chimeric decoy receptor trap fusion protein on retina neovascularization by dual blockage of VEGF and FGF-2 | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences | 29715501 | Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from AllCells (Shanghai, China) and maintained in a complete HUVEC medium (Cat# H-004, AllCells) | Full Paper |
2018 | Yen, J;Fiorino, M;Liu, Y;Paula, S;Clarkson, S;Quinn, L;Tschantz, WR;Klock, H;Guo, N;Russ, C;Yu, VWC;Mickanin, C;Stevenson, SC;Lee, C;Yang, Y; | TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem Cells | Scientific reports | 30389991 | Cryopreserved bone marrow derived CD34 + HSPCs were purchased from Lonza or AllCells and thawed according to vendors' instructions. Cells were cultured at 37 C 5% CO 2 in Save | Full Paper |
2018 | Han, SS;Shim, HE;Park, SJ;Kim, BC;Lee, DE;Chung, HM;Moon, SH;Kang, SW; | Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking | Scientific reports | 30181604 | The protocol was approved by the Committee on Biological Research of Korea Institute of Toxicology and Institutional Review Board (P01-201509-41-001). hUCB-EPCs culture hUCB-EPCs were purchased from AllCells (Alameda, CA, USA). hUCB-EPCs culture wer | Full Paper |
2018 | Burgenson, D;Gurramkonda, C;Pilli, M;Ge, X;Andar, A;Kostov, Y;Tolosa, L;Rao, G; | Rapid recombinant protein expression in cell-free extracts from human blood | Scientific reports | 29934577 | anced the expression of Nluc, but RNase inhibitor did not have a significant effect (Fig. 2C,D).Figure 1Production of human leukocyte extract and Nluc expression. (A) Leukopak was purchased form the AllCells, Inc. (B) The cells were clarified by cent | Full Paper |
2018 | Valton, J;Guyot, V;Boldajipour, B;Sommer, C;Pertel, T;Juillerat, A;Duclert, A;Sasu, BJ;Duchateau, P;Poirot, L; | A Versatile Safeguard for Chimeric Antigen Receptor T-Cell Immunotherapies | Scientific reports | 29895885 | Primary T-cells isolated from PBMC (ALLCELLS) were activated and transfected according to previously described methods 13 . Four days after their activation by Dynabeads human T Save | Full Paper |
2018 | Gaudin, A;Tolar, M;Peters, OA; | Lipoxin A4 Attenuates the Inflammatory Response in Stem Cells of the Apical Papilla via ALX/FPR2 | Scientific reports | 29892010 | eated with mitomycin C (Sigma-Aldrich) at the final concentration of 25 μg/mL for 30 min, and then washed 4 times with PBS + 10% Human Serum. Human peripheral blood mononuclear cells (PBMC) (AllCells, Alameda, CA, USA) in dilution ratios 1:1, 1:5 and | Full Paper |
2018 | Lim, SH;Sun, Y;Thiruvallur Madanagopal, T;Rosa, V;Kang, L; | Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular Modification | Scientific reports | 29371611 | 215 nm. A calibration curve was conducted using the respective standard solutions from 1 ppm to 1000 ppm. DPSC Cell Culture Primary human dental pulp stem cells (DPSC) from a single donor (DPF003, AllCells, USA) were cultured in a complete growth me | Full Paper |
2018 | Ruppert, KA;Nguyen, TT;Prabhakara, KS;Toledano Furman, NE;Srivastava, AK;Harting, MT;Cox, CS;Olson, SD; | Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Modify Microglial Response and Improve Clinical Outcomes in Experimental Spinal Cord Injury | Scientific reports | 29323194 | hMSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old male (AllCells, Alameda, CA) using density centrifugation and plastic adherence Save | Full Paper |
2018 | Li, C;Sunderic, K;Nicoll, SB;Wang, S; | Downregulation of Heat Shock Protein 70 Impairs Osteogenic and Chondrogenic Differentiation in Human Mesenchymal Stem Cells | Scientific reports | 29323151 | Human MSCs were isolated from fresh human bone marrow purchased from AllCells (Alameda, CA) according to manufacturer's instructions as previously described 2 and cultured Save | Full Paper |
2018 | Bloom, DD;Reshetylo, S;Nytes, C;Goodsett, CT;Hematti, P; | Blockade of BAFF Receptor BR3 on T Cells Enhances Their Activation and Cytotoxicity | Journal of immunotherapy (Hagerstown, Md. : 1997) | 29309290 | Primary human T cells were obtained from leukopheresis products purchased from AllCells, LLC (Alameda, CA) or Key Biologics, LLC (Memphis, TN). Upon arrival, PBLs were isolated Save | Full Paper |
2018 | Mair, F;Prlic, M; | OMIP-044: 28-color immunophenotyping of the human dendritic cell compartment | Cytometry. Part A : the journal of the International Society for Analytical Cytology | 29356334 | Cryopreserved PBMCs were obtained from AllCells, stained and subsequently aquired on a BD FACSymphony. Optimal PMT voltages were determined by voltage titration experiments and iterative refinement. All reagents were titrated prior to use. For Figure | Full Paper |
2018 | Doering, CB;Denning, G;Shields, JE;Fine, EJ;Parker, ET;Srivastava, A;Lollar, P;Spencer, HT; | Preclinical Development of a Hematopoietic Stem and Progenitor Cell Bioengineered Factor VIII Lentiviral Vector Gene Therapy for Hemophilia A | Human gene therapy | 30160169 | Genetically modified, autologous hematopoietic stem and progenitor cells (HSPCs) represent a new class of genetic medicine. Following this therapeutic paradigm, we are developing a Save | Full Paper |
2018 | Liu, XY;Xu, HX;Li, JK;Zhang, D;Ma, XH;Huang, LN;Lü, JH;Wang, XZ; | Neferine Protects Endothelial Glycocalyx via Mitochondrial ROS in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome | Frontiers in physiology | 29520236 | Human umbilical vein endothelial cells (HUVECs) were grown to confluence and cultured in 25 cm 2 flasks supplemented with complete culture medium (AllCells, Shanghai, China) in a Save | Full Paper |
2018 | Zeng, Y;Dabay, M;George, V;Seetharaman, S;de Arruda Indig, M;Graminske, S;Kimpel, N;Schmidt, A;Boerner, A;Paradiso, S;Delman, T;Li, Y;Litvak, V;Oreizy, F;Chen, A;Saleminik, M;Mosqueda, F;Lin, A;Judge, K; | Comparison of Flow Cytometric Methods for the Enumeration of Residual Leucocytes in Leucoreduced Blood Products: A Multicenter Study | Cytometry. Part A : the journal of the International Society for Analytical Cytology | 29345745 | Per enrollment needs, leucoreduced PLTs and RBCs with autologous white blood cell sources were also purchased from qualified vendors that produce apheresis PLTs and RBCs through standard procedures (AllCells, LLC, Alameda, CA; Stanford Blood Center, | Full Paper |
2018 | Song, B;Chen, Y;Liu, Y;Wan, C;Zhang, L;Zhang, W; | NPAS2 regulates proliferation of acute myeloid leukemia cells via CDC25A-mediated cell cycle progression and apoptosis | Journal of cellular biochemistry | 30536616 | NHC cell specimens were separated from normal cells in bone marrow bought from AllCells, LLC (Emeryville, CA). Cells were preserved in 5% CO 2 at 37C in RPMI-1640 media Save | Full Paper |
2018 | Lewis, G;Christiansen, L;McKenzie, J;Luo, M;Pasackow, E;Smurnyy, Y;Harrington, S;Gregory, P;Veres, G;Negre, O;Bonner, M; | Staurosporine Increases Lentiviral Vector Transduction Efficiency of Human Hematopoietic Stem and Progenitor Cells | Molecular therapy. Methods & clinical development | 30038935 | CD34-enriched mPB samples from healthy human donors were obtained from AllCells (Emeryville, CA) and Key Biologics (Memphis, TN). Research-grade staurosporine was obtained Save;apeutics. Materials and Methods Cell Culture Research-grade BlaM-conta | Full Paper |
2018 | Mendoza, M;Ballesteros, A;Qiu, Q;Pow Sang, L;Shashikumar, S;Casares, S;Brumeanu, TD; | Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD) | Human vaccines & immunotherapeutics | 29135340 | Briefly, HLA-DRB1*0401 + umbilical cord bloods (UCB) purchased from AllCells and Promocell were enriched for CD34 + stem cells to more than 60% when using EasySep, Human Save | Full Paper |
2018 | Low, EK;Brudvik, E;Kuhlman, B;Wilson, PF;Almeida-Porada, G;Porada, CD; | Microgravity Impairs DNA Damage Repair in Human Hematopoietic Stem/Progenitor Cells and Inhibits Their Differentiation into Dendritic Cells | Stem cells and development | 29901426 | Healthy human BM mononuclear cells (BMMNC) were purchased from AllCells, LLC (Alameda, CA). CD34+ HSC were isolated from BMMNC using anti-human CD34 MicroBeads and the MiniMACS magnetic-based positive-selection system (Miltenyi Biotec, Inc., Auburn, | Full Paper |
2018 | Almeida-Porada, G;Rodman, C;Kuhlman, B;Brudvik, E;Moon, J;George, S;Guida, P;Sajuthi, SP;Langefeld, CD;Walker, SJ;Wilson, PF;Porada, CD; | Exposure of the Bone Marrow Microenvironment to Simulated Solar and Galactic Cosmic Radiation Induces Biological Bystander Effects on Human Hematopoiesis | Stem cells and development | 29698131 | In brief, upon return from BNL, MSC from each irradiation group were overlaid with human HSC (CD34+ BM-derived cells from healthy adult donors, purchased from AllCells, LLC, or Save | Full Paper |
2018 | Sebastian, AA;Kannan, TP;Norazmi, MN;Nurul, AA; | Interleukin-17A promotes osteogenic differentiation by increasing OPG/RANKL ratio in stem cells from human exfoliated deciduous teeth (SHED) | Journal of tissue engineering and regenerative medicine | 29774992 | SHED were purchased from ALLCELLS, Alameda, CA, USA (Catalogue No. DP004F; lot number SHED092211-01). SHED from Passages 4 to 6 were used in the study. SHED were cultured in alpha minimum essential medium (Gibco, USA) supplemented with 10% v/v fetal | Full Paper |
2018 | Gregg, KA;Harberts, E;Gardner, FM;Pelletier, MR;Cayatte, C;Yu, L;McCarthy, MP;Marshall, JD;Ernst, RK; | A lipid A-based TLR4 mimetic effectively adjuvants a Yersinia pestis rF-V1 subunit vaccine in a murine challenge model | Vaccine | 29861179 | Co-stimulatory marker surface expression assays were contracted to and completed by AllCells using standard protocols. Briefly, ex vivo human monocytes were differentiated to Save | Full Paper |
2018 | Kanagasabai, R;Karmahapatra, S;Kientz, CA;Yu, Y;Hernandez, VA;Kania, EE;Yalowich, JC;Elton, TS; | The Novel C-terminal Truncated 90-kDa Isoform of Topoisomerase IIα (TOP2α/90) Is a Determinant of Etoposide Resistance in K562 Leukemia Cells via Heterodimerization with the TOP2α/170 Isoform | Molecular pharmacology | 29514855 | Human cord blood mononuclear cells were purchased from ALLCELLS (Alameda, CA). Deidentified acute myeloid leukemia (AML) patient samples were obtained from The Ohio State Save | Full Paper |
2018 | Liu, L;Puri, N;Raffaele, M;Schragenheim, J;Singh, SP;Bradbury, JA;Bellner, L;Vanella, L;Zeldin, DC;Cao, J;Abraham, NG; | Ablation of soluble epoxide hydrolase reprogram white fat to beige-like fat through an increase in mitochondrial integrity, HO-1-adiponectin in vitro and in vivo | Prostaglandins & other lipid mediators | 30041041 | Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA, USA). After thawing, mononuclear cells were re-suspended in a-minimal essential medium (a-MEM, Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat-in-a | Full Paper |
2018 | Wang, J;Xu, J;Peng, Y;Xiao, Y;Zhu, H;Ding, ZM;Hua, H; | Phosphorylation of extracellular signal-regulated kinase as a biomarker for cannabinoid receptor 2 activation | Heliyon | 30450437 | tal calf serum, 100 U/ml penicillin/streptomycin, and 2 mM l-glutamine at 37 °C in 5% CO2/95% air. 2.2 Human whole blood cell culture Human whole blood cells were purchased from commercial sources (Allcells, Alameda, CA, USA or Zen-Bio, Research Tri | Full Paper |
2018 | Wiredja, DD;Tabler, CO;Schlatzer, DM;Li, M;Chance, MR;Tilton, JC; | Global phosphoproteomics of CCR5-tropic HIV-1 signaling reveals reprogramming of cellular protein production pathways and identifies p70-S6K1 and MK2 as HIV-responsive kinases required for optimal infection of CD4+ T cells | Retrovirology | 29970186 | Normal donor samples were de-identified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated by Save | Full Paper |
2018 | Friesen, WJ;Johnson, B;Sierra, J;Zhuo, J;Vazirani, P;Xue, X;Tomizawa, Y;Baiazitov, R;Morrill, C;Ren, H;Babu, S;Moon, YC;Branstrom, A;Mollin, A;Hedrick, J;Sheedy, J;Elfring, G;Weetall, M;Colacino, JM;Welch, EM;Peltz, SW; | The minor gentamicin complex component, X2, is a potent premature stop codon readthrough molecule with therapeutic potential | PloS one | 30359426 | ). Fold suppression over background was calculated as (aminoglycoside light units/PBS light units) [7]. Cytotoxicity assays Frozen human Peripheral Blood Mononuclear Cells (PBMCs) were obtained from ALLCELLS (Alameda, CA, Cat# PB003F) from a single | Full Paper |
2018 | Caseiro, AR;Ivanova, G;Pedrosa, SS;Branquinho, MV;Georgieva, P;Barbosa, PP;Santos, JD;Magalhães, R;Teixeira, P;Pereira, T;Maurício, AC; | Human umbilical cord blood plasma as an alternative to animal sera for mesenchymal stromal cells in vitro expansion - A multicomponent metabolomic analysis | PloS one | 30304014 | , 29, 47-49]. UC-MSCs and DPSCs were maintained at 37°C and 95% humidified atmosphere with 5% CO2 environment. UC-MSCs were obtained from PromoCell, Cat. C-12971; Lot No. 1112304.2) and DPSCs from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01). | Full Paper |
2018 | Oshima, T;Miyashita, H;Ishimura, Y;Ito, Y;Tanaka, Y;Hori, A;Kokubo, T;Kurokawa, T; | Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey | PloS one | 29723247 | Human peripheral blood mononuclear cells (PBMC) purchased from AllCells, LLC and were cultured in RPMI1640 medium containing 10% fetal bovine serum, 0.1 nM human IL-2 ( Save | Full Paper |
2018 | Del'Guidice, T;Lepetit-Stoffaes, JP;Bordeleau, LJ;Roberge, J;Théberge, V;Lauvaux, C;Barbeau, X;Trottier, J;Dave, V;Roy, DC;Gaillet, B;Garnier, A;Guay, D; | Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells | PloS one | 29617431 | N). Myoblasts are primary human cells kindly provided by Professor J.P. Tremblay (Université Laval, Quebec, QC, Canada). Normal Peripheral Blood CD56+ NK cells are natural killer cells obtained from Allcells Inc. (Alameda, CA, USA). Cortical cells ar | Full Paper |
2018 | Sunderic, K; | Effects of Thermal Dosing and Ultrasound on Osteogenic Differentiation of Human Mesenchymal Stem Cells in 2D and 3D Culture | Thesis | Human bone marrow explanted from the iliac crest of donors was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the RosetteSep MSC enrichment cocktail (StemCell Technologies, Vancouver, Canada) per manufac | Full Paper | |
2018 | Nair, RR;Piktel, D;Geldenhuys, WJ;Gibson, LF; | Combination of cabazitaxel and plicamycin induces cell death in drug resistant B-cell acute lymphoblastic leukemia | Leukemia research | 30103201 | Primary immune cells CD3 + T cells, CD19 + B cells, peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) were all purchased from ALLCELLS and Save | Full Paper |
2018 | Palasuk, J;Windsor, LJ;Platt, JA;Lvov, Y;Geraldeli, S;Bottino, MC; | Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion | Clinical oral investigations | 28965247 | Next, the eluates were serially diluted to 32-, 64-, and 128-fold [21]. Human dental pulp stem cells ([hDPSCs] AllCells LLC, Alameda, CA, USA) were cultured under standard conditions in a humidifier incubator (5% CO2/95% air) at 37 °C in low-glucose | Full Paper |
2018 | Sasaki, E;Momose, H;Hiradate, Y;Ishii, KJ;Mizukami, T;Hamaguchi, I; | In vitro marker gene expression analyses in human peripheral blood mononuclear cells: A tool to assess safety of influenza vaccines in humans | Journal of immunotoxicology | 29521144 | PBMC obtained from healthy adult subjects were purchased from AllCells (Alameda, CA) and cultured in complete RPMI, ie RPMI 1640 supplemented with 10% fetal calf serum (FCS), Save | Full Paper |
2018 | Tian, H;Li, S;Yu, K; | DJ‑1 alleviates high glucose‑induced endothelial cells injury via PI3K/Akt‑eNOS signaling pathway | Molecular medicine reports | 29115508 | HUVECs were purchased from AllCells Biotechnology Co., Ltd. (Shanghai, China). The culture medium was DMEM with either 5.5 mM (control group) or 25 mM (HG group) D-glucose, containing 10% fetal bovine serum, penicillin (100 U/ml) and streptomycin (10 | Full Paper |
2018 | Hu, X;Hu, T;Guan, G;Yu, S;Wu, Y;Wang, L; | Control of weft yarn or density improves biocompatibility of PET small diameter artificial blood vessels | Journal of biomedical materials research. Part B, Applied biomaterials | 28456004 | The HUVECs (HUVEC001), provided by Allcells Company (Shanghai, China), were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Gibco) at 37°C in a 5% CO2 incubator. Before se | Full Paper |
2018 | Joslin, J;Gilligan, J;Anderson, P;Garcia, C;Sharif, O;Hampton, J;Cohen, S;King, M;Zhou, B;Jiang, S;Trussell, C;Dunn, R;Fathman, JW;Snead, JL;Boitano, AE;Nguyen, T;Conner, M;Cooke, M;Harris, J;Ainscow, E;Zhou, Y;Shaw, C;Sipes, D;Mainquist, J;Lesley, S; | A Fully Automated High-Throughput Flow Cytometry Screening System Enabling Phenotypic Drug Discovery | SLAS discovery : advancing life sciences R & D | 29843542 | Primary human CD4 T cells were purified using a CD4+ T-cell isolation kit (130-096-533, Miltenyi, Auburn, CA) from cells obtained from leukapheresis of a normal human donor (AllCells Save;reg (T-Regulatory) Screen Primary human CD4 T cells were puri | Full Paper |
2018 | Barve, A;Casson, L;Krem, M;Wunderlich, M;Mulloy, JC;Beverly, LJ; | Comparative utility of NRG and NRGS mice for the study of normal hematopoiesis, leukemogenesis, and therapeutic response | Experimental hematology | 30125602 | Cell-line-derived xenografts (CDXs) or patient-derived xenografts (PDXs) in immune-deficient mice have revolutionized our understanding of normal and malignant human Save | Full Paper |
2018 | Reshetnikova, E;Guselnikov, S;Volkova, O;Baranov, K;Taranin, A;Mechetina, L; | B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans | Cytometry. Part B, Clinical cytometry | 29236355 | For this purpose, pDC cDNA from the BDCA4+ (CD304+) pDCs isolated from the peripheral blood of a healthy donor (cat. no. cDNA-PB006) was purchased from AllCells, LLC (Alameda, CA, USA). Human PBMC and spleen total RNA was isolated using Qiagen RNAeas | Full Paper |
2018 | Ikutani, M;Ogawa, S;Yanagibashi, T;Nagai, T;Okada, K;Furuichi, Y;Takatsu, K; | Elimination of eosinophils using anti-IL-5 receptor alpha antibodies effectively suppresses IL-33-mediated pulmonary arterial hypertrophy | Immunobiology | 29269115 | Human PBMCs (AllCells, Alameda, CA, USA) and Y16 cells were used as effector cells and target cells, respectively. Target cells (2 × 104 cells) were incubated with the effector cells (5 × 105 cells) and chimeric antibodies in a 96-well U-form plate a | Full Paper |
2018 | Sun, K;Mikule, K;Wang, Z;Poon, G;Vaidyanathan, A;Smith, G;Zhang, ZY;Hanke, J;Ramaswamy, S;Wang, J; | A comparative pharmacokinetic study of PARP inhibitors demonstrates favorable properties for niraparib efficacy in preclinical tumor models | Oncotarget | 30647846 | m to assess human megakaryocyte-specific development In vitro human megakaryocyte differentiation was assessed using a liquid culture system in which the human bone marrow CD34+ cells (lot # 0150114, ReachBio) derived from normal bone marrow were use | Full Paper |
2018 | Liao, C; | Regulation of Stress Erythropoiesis and the Niche: A Noval Role of Selenoproteins | Thesis | Transferrin (200µg/mL), Scf (50ng/mL), Bmp4 (15ng/mL), Gdf15 (30ng/mL), and Shh (25ng/mL), for 5 to 7 days to enrich for SEPs. Human BM cells from three subjects were purchased from Reachbio Research Labs. Human BM culture was the same condition with | Full Paper | |
2018 | Wu, H;He, L;Shi, J;Hou, X;Zhang, H;Zhang, X;An, Q;Fan, F; | Resveratrol inhibits VEGF-induced angiogenesis in human endothelial cells associated with suppression of aerobic glycolysis via modulation of PKM2 nuclear translocation | Clinical and experimental pharmacology & physiology | 30044005 | medium (DMEM) (Hyclone, Pittsburgh, PA, USA) supplemented with 10% foetal bovine serum (FBS; Cellmax, Beijing, China), 100 U/mL penicillin and 100 μg/mL streptomycin, and grown in a 95% air and 5% CO2 humidified atmosphere at 37°C, Primary HUVECs wer | Full Paper |
2018 | Ni, R;Luo, L; | A noncanonical function of histidyl-tRNA synthetase: inhibition of vascular hyperbranching during zebrafish development | FEBS open bio | 29744287 | loheximide (CHX; Sigma) from 36 hpf to observation, or treated with DMSO at the same concentration as control. Immunofluorescence staining and imaging Human umbilical vein endothelial cells (HUVECs, ALLCELLS) were cultured according to standard prot | Full Paper |
2018 | Han, XB;Tan, Y;Fang, YQ;Li, F; | Protective effects of celastrol against γ irradiation-induced oxidative stress in human umbilical vein endothelial cells | Experimental and therapeutic medicine | 30116323 | anghai Xingya Purify Material Ltd. (Shanghai, China). Western blotting detection ECL Reagent was purchased from GE Healthcare China Co. Ltd. (Beijing, China). Cell culture HUVECs were purchased from AllCells Biotech Ltd. (Shanghai, China), and grown | Full Paper |
2018 | Varma, DM;DiNicolas, MS;Nicoll, SB; | Injectable, redox-polymerized carboxymethylcellulose hydrogels promote nucleus pulposus-like extracellular matrix elaboration by human MSCs in a cell density-dependent manner | Journal of biomaterials applications | 30326804 | hMSCs were isolated from adult human bone marrow (AllCells, Emeryville, CA) via enriched density gradient centrifugation as previously described. Briefly, a hMSC Enrichment Save | Full Paper |
2018 | Zhao, X;Gao, J;Hu, X;Guo, H;Wang, F;Qiao, Y;Wang, L; | Collagen/Polyethylene Oxide Nanofibrous Membranes with Improved Hemostasis and Cytocompatibility for Wound Dressing | Applied Sciences | Human umbilical vein endothelial cells (HUVEC001) were supplied by Allcells Company (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, Shanghai, China) and 1% penicillin-streptomycin ( | Full Paper | |
2018 | Thomson-Luque, R;Wang, C;Ntumngia, FB;Xu, S;Szekeres, K;Conway, A;Adapa, SR;Barnes, SJ;Adams, JH;Jiang, RHY; | In-depth phenotypic characterization of reticulocyte maturation using mass cytometry | Blood cells, molecules & diseases | 30007855 | ). Reticulocytes and mature RBCs were kept at 4 °C in MIM until used, always before 2 days post isolation. One bone marrow reticulocyte preparation was obtained from unprocessed whole aspirates (ALLCELLS) following the same protocol as for peripheral | Full Paper |
2018 | Wang, J;Xu, J;Liu, J;Zhu, H;Peng, Y;Ding, ZM;Hua, H; | Genetic Variant Q63R of Cannabinoid Receptor 2 Causes Differential ERK Phosphorylation in Human Immune Cells | Genetic testing and molecular biomarkers | 29694791 | All human PBMCs were from commercial sources (provided by Allcells or Zenbio). Human PBMCs were thawed and cultured in the culture medium (RPMI-1640 containing 2 mM L- Save | Full Paper |
2018 | Gao, X;Yourick, JJ;Sprando, RL; | Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel | Stem cell research | 30099336 | Whole blood collection from healthy donors was conducted by AllCells with informed consent under the Protocol #7000-SOP-049 Version 2.0 approved by Alpha Independent Review Save | Full Paper |
2018 | Pan, H;Zhang, M; | Serum of coronary atherosclerotic heart disease patients induces oxidative stress injury on endothelial cells | Pteridines | HUVECs were purchased from AllCells (AllCells Technology, Shanghai, China). Cells were grown in complete medium (HUVEC-004, AllCells) in an incubator with 5% CO2 at 37 °C. HUVECs were treated with the serum from patients with CAD for 24 h. HUVECs wer | Full Paper | |
2018 | Enezei, H;Ahmad, A;Takeuchi, K;Suzuki, J;Khamis, M;Razak, N;Rahman, R;Qabbani, A;Abdulhameed, E;Sugita, Y;Maeda, H;Alam, M; | Osteoinductive Activity of Bone Scaffold Bioceramic Companied with Control Release of VEGF Protein Treated Dental stem cells as A New Concept for Bone Regeneration: Part II | Journal of Hard Tissue Biology | Dental Stem Cells (DSCs) AllCells, LLC Company, Emeryville, CA, USA Dulbecco’s modified Eagles medium (DMEM) high glucose(4.5g/L) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Fetal bovine serum (FBS) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, | Full Paper | |
2018 | Sasaki, E;Momose, H;Hiradate, Y;Furuhata, K;Mizukami, T;Hamaguchi, I; | Development of a preclinical humanized mouse model to evaluate acute toxicity of an influenza vaccine | Oncotarget | 29899819 | Human PBMCs from one healthy donor (24 years old, male adult Caucasian) were obtained from AllCells (Alameda, CA, USA) and were subjected to the experiment shown in Figures 1 Save | Full Paper |
2018 | Gołąb, K;Grose, R;Placencia, V;Wickrema, A;Solomina, J;Tibudan, M;Konsur, E;Ciepły, K;Marek-Trzonkowska, N;Trzonkowski, P;Millis, JM;Fung, J;Witkowski, P; | Cell banking for regulatory T cell-based therapy: strategies to overcome the impact of cryopreservation on the Treg viability and phenotype | Oncotarget | 29515766 | We obtained de-identified human cell leukapheresis product for all the experiments from the commercial vendor AllCells, Alameda, CA, USA, therefore the study was exempt from the Save | Full Paper |
2018 | Shehadat, SA;Gorduysus, MO;Hamid, SSA;Abdullah, NA;Samsudin, AR;Ahmad, A; | Optimization of scanning electron microscope technique for amniotic membrane investigation: A preliminary study | European journal of dentistry | 30369805 | que by a cell scraper (TPP, Europe/Switzerland) was applied when necessary to remove the epithelial cells that could not be washed away by phosphate-buffered saline (PBS). Deciduous teeth stem cells (AllCells, USA) were seeded on the de-epithelialize | Full Paper |
2018 | Subhi, H;Reza, F;Husein, A;Al Shehadat, SA;Nurul, AA; | Gypsum-Based Material for Dental Pulp Capping: Effect of Chitosan and BMP-2 on Physical, Mechanical, and Cellular Properties | International journal of biomaterials | 30147725 | SHED were purchased from AllCells, USA. The cells at passage 8 were cultured in Alpha minimum essential medium [????-MEM; Invitrogen, USA (GIBCO)] supplemented with 10% fetal Save | Full Paper |
2018 | Ross, CL;Pettenati, MJ;Procita, J;Cathey, L;George, SK;Almeida-Porada, G; | Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal Cells | Global advances in health and medicine | 29796339 | me stability were not affected or altered by ELF-EMF, when used at the tested field strength and frequency. Materials and Methods Cell Culture Human bone mononuclear cells (BMNCs) were obtained from AllCells (Alameda, CA). BMNCs were enriched for th | Full Paper |
2018 | Subhi, H;Reza, F;Husein, A;Nurul, AA; | Cytotoxicity of gypsum-based biomaterial for direct pulp capping using stem cells from human exfoliated deciduous teeth | Journal of conservative dentistry : JCD | 29628642 | epare the biomaterial. The compositions of the Gyp-CHT biomaterial are shown in Table 1. Table 1 The compositions of the experimental biomaterial Cell culture preparation SHED were purchased from AllCells, USA. The cells at passage eight were cul | Full Paper |
2018 | Luddin, N;Ahmed, H;Kannan, T;Mokhtar, K;Ahmad, A; | Calcium chloride dihydrate affects the biological properties of white mineral trioxide aggregate on dental pulp stem cells: An in vitro study | Saudi Endodontic Journal | molars (adult) and characterized were purchased from AllCells (CA, USA). The complete (AllCells), with 50 ml of human mesenchymal stem cell stimulatory supplements (AllCells). Save | Full Paper | |
2018 | Goncalves, K;Hoban, M;Proctor, J;Hyzy, S;Adams, H;Gardner, J;Boitano, A;Cooke, M; | Comparison of hematopoietic stem cell expansion methods reveals that aryl hydrocarbon receptor inhibition optimally expands CD90 engrafting cells | SSRN Electronic Journal | Human cord blood CD34+ cells or mobilized peripheral blood CD34+ cells (male or female) were purchased from AllCells. Cell number, purity, and viability was confirmed at time of thaw. Cell counts were obtained using TruCount Tubes (BD). Cell viabilit | Full Paper | |
2018 | laura, ;Danilevsky, M;Li, Y;Reiss, F;Zhu, H; | SystemT: Declarative Text Understanding for Enterprise | Proceedings of the 2018 Conference of the North American Chapter of the Association for Computational Linguistics: Human Language Technologies, Volume 3 (Industry Papers) | able in AQL, including dictionary matching (AssetClassSuffixes), core NLP operators such as Semantic Role Labeling (SRL) (Verbs, Arguments), and document structure operators such as table structure understanding (AllCells). Sec. 3 details the data mo | Full Paper | |
2018 | Abhyankar, V; | The chemokine and cytokine responses of a keratinocyte, dendritic cell, and T-cell co-culture model treated with P. gingivalis hemagglutinin B (HagB) | University of Iowa | Background: P. gingivalis, a non-motile, rod-shaped, anaerobic, Gram-negative bacterium is one of the principal sources of periodontal disease. It possesses a number of potential Save | Full Paper | |
2018 | Sabhachandani, ; | Development of microfluidic microanalysis systems for cancer therapeutic screening | Northeastern University | cells were purchased from AllCells (#PB009-3F, AllCells, Alameda, CA). Cells were maintained in RPMI-1640 medium supplemented with 10% Fetal Bovine Serum and 1% Antibiotic-Antimycotic solution (Corning Cellgro, Manassas, VA). Primary human CD56+ NK c | Full Paper | |
2018 | Bates, A; | Matrix metalloproteinase and cytokine profiles from cell co-cultures and their role in oral inflammation and head and neck cancer | University of Iowa | Human monocyte-derived immature myeloid dendritic cells (DC, AllCells, Alameda, CA) were positively identified by flow cytometry and contained DC of myeloid origin: 31.5% CD1c+/79.1% CD11c+ cells and 54.4% CD141+ /79.1% CD11c+ cells (Human myeloid DC | Full Paper | |
2018 | Nada, M; | Adoptive cancer immunotherapy with human Vγ2vδ2 T cells | University of Iowa | Ex vivo expansion of Vγ2Vδ2 T cells PBMC from random healthy adult donors were obtained by density centrifugation over Ficoll-Hypaque (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) of either random donor leukopaks (12 donors) (obtained from AllCe | Full Paper | |
2018 | Twyman, RM;Wisden, W; | Development, Molecular Aspects | Advance Molecular Biology | A fate map is a map of an egg or embryo which shows the fates of allcells A cell which will form gametes. Germ cells usually segregate from somatic cells early in animal development Save | Full Paper | |
2018 | Riepe, C;Zelin, E;Wyman, S;Nguyen, D;Liang, J;Frankino, P;Meacham, Z;Vu, J;Marson, A;Ingolia, N;Corn, J; | Double Stranded DNA Breaks and Genome Editing Trigger Ribosome Remodeling and Translational Shutdown | bioRxiv | Mobilized Peripheral Blood CD34+ Stem/Progenitor Cells (AllCells) were cultured in StemSpan™ Serum Free Expansion Media II (STEMCELL Technologies), StemSpan™ StemSpan(tm) CC110 (STEMCELL Technologies), 1% Pen/Strep | Full Paper | |
2018 | Mimitou, E;Cheng, A;Montalbano, A;Hao, S;Stoeckius, M;Legut, M;Roush, T;Herrera, A;Papalexi, E;Ouyang, Z;Satija, R;Sanjana, N;Koralov, S;Smibert, P; | Expanding the CITE-seq tool-kit: Detection of proteins, transcriptomes, clonotypes and CRISPR perturbations with multiplexing, in a single assay | bioRxiv | DMSO and aliquots of 1-1.5 million cells were thawed on the day of the experiment. Peripheral blood mononuclear cells (PBMCs) were obtained cryopreserved from AllCells (USA) and used immediately after thawing. NIH-3T3 (mouse) cells expressing non-tar | Full Paper | |
2018 | Setty, M;Kiseliovas, V;Levine, J;Gayoso, A;Mazutis, L;Pe’er, D; | Palantir characterizes cell fate continuities in human hematopoiesis | bioRxiv | Cryopreserved bone marrow stem/progenitor CD34+ cells from healthy donors were purchased from AllCells, LLC. (Cat. No. ABM022F) and stored in vapor phase nitrogen until use. Typical for scRNA-seq, a vial was removed from the storage and immediately t | Full Paper | |
2018 | Shin, J;Wyman, S;Dewitt, M;Bray, N;Vu, J;Corn, J; | Controlled cycling and quiescence enables homology directed repair in engraftment-enriched adult hematopoietic stem and progenitor cells | bioRxiv | Cryopreserved wildtype human mobilized peripheral blood CD34+ HSPCs from multiple volunteer donors including male and female whose age ranged from 20-35 were purchased from Allcells, Inc | Full Paper | |
2018 | Wong, D; | Immuno-Chemotherapeutic Platinum(IV) Prodrugs of Cisplatin as Multimodal Anticancer Agents | Rethinking Platinum Anticancer Drug Design: Towards Targeted and Immuno-chemotherapeutic Approaches | Prior to activation, frozen PBMCs (AllCells LLC, USA) were thawed and washed twice with complete RPMI to remove freezing medium. 4.1 × 10 6 PBMCs were incubated in 5 mL of Save | Full Paper | |
2018 | Johnsen, KH; | Performance Analysis and Optimization of the Equelle DSL | Thesis | Gradient() takes a CollOfScalar as argument which is defined on AllCells and returns a CollOfScalar which is defined on InteriorFaces. The Divergence() function is used to calculate the Save | Full Paper | |
2018 | Ganaie, SS; | Essential Host Factors for Human Parvovirus B19 Replication | Thesis | We purchased CD34+ hematopoietic stem cells, which were isolated from bone marrow of a healthy human donor, from AllCells LLC (Alameda, CA) without any identification information on the cells, and, therefore, an institutional review board (IRB) revie | Full Paper | |
2018 | Fink, EC; | Investigating the Mechanism and Species-Specificity of Thalidomide Derivatives | Thesis | Control cord-blood CD34+ cells were isolated as above. Adult bone marrow CD34+ cells were purchased as single-donor lots from AllCells (Alameda, CA). The number of replicates for each patient sample, vector, and treatment was limited by the number of | Full Paper | |
2018 | Ahmed, MA; | Exploring the impact of hypoxia mimetic agents on multipotent stem cell biology | Thesis | Fresh human bone marrow aspirate ABM001-1 ALLCELLS Gel Loading Buffer (0.05% bromophenol blue, 40% sucrose, 0.1 M EDTA, 0.5% SDS) G2526 Sigma-Aldrich Glycine 50046 SIGMA-ALDRICH Horse serum H 1270 Sigma-Aldrich GAP-43 Antibody NB300-143 Novus Biologi | Full Paper | |
2018 | Sebastian Kettinger, N; | Investigating the HIV Reservoir in Hematopoietic Stem and Progenitor Cells | Thesis | (eBioscience), and 7-Aminoactinomycin D (7-AAD). Healthy donor BMMCs (AllCells) were stained with R-phycoerythrin-conjugated anti-CD133, R-phycoerythrin-Cy7-conjugated anti-CD34 (BD), and 7-AAD. Samples were analyzed on a BD FACSCan | Full Paper | |
2018 | Al-Jumaily, RMK; | Molecular cytology of hypoxic cancer and stem cells: an epigenetic approach | Thesis | 1 BMA-16 Human Bone Marrow, Male, Age-29 years Lonza, MD, USA 2 BMA-20 Human Bone Marrow, Female, Age-50 years AllCells, LLC, SA 3 BMA-25 Human Bone Marrow, Male, Age-27 years Lonza, MD, USA | Full Paper | |
2018 | Becker, AH; | Epigenetic and Gene Expression Changes Mediated by Histone H3 Methylation in Acute Myeloid Leukemia | Thesis | 2.1.2 Bone marrow CD34+ cells Cryopreserved bone marrow CD34+ cells from normal donors were obtained from Allcells (Alameda, CA). 2.1.3 K562 cells Human K562 cells (ATCC CCL-243) were grown in RPMI 1640 GlutaMAX medium (Gibco, Thermo Fisher Scientifi | Full Paper | |
2018 | Bartenstein, M; | Symptomatic treatment of myelofibrosis by TGF-β1 signaling inhibition | Thesis | University of Chicago. Cells were purified immune-magnetically from granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood of healthy donors purchased from AllCells, Inc. using a paramagnetically labeled mouse anti-human CD34 antibo | Full Paper | |
2018 | Siriwon, N; | Engineered CAR-T Cells for Treatment of Solid Cancers | Thesis | Frozen human PBMCs were obtained from AllCells (Alameda, CA). PBMCs were thawed in TCM and rested overnight. Before retroviral transduction, PBMCs were activated for 2 days by culturing with Dynabeads human T-activator CD3/CD28 (Thermo Fisher Scienti | Full Paper | |
2018 | Seet, C; | In Vitro Generation of Adaptive Immunity from Hematopoietic Stem Cells | Thesis | deliveries at UCLA. Bone marrow (BM) was obtained from healthy adult donors (ages 18-51) through discarded material from allogeneic BM donor harvests at UCLA or purchased from AllCells Inc. (Alameda, CA). G-CSF mobilized peripheral blood was obtained | Full Paper | |
2018 | Lin, HA; | Development and Functional Evaluation of Injectable Cellulose-based Hydrogels as Nucleus Pulposus Replacements for Intervertebral Disc Repair | Thesis | 2.2.4. Cell-laden hydrogel fabrication and characterization 2.2.4.1. Primary cell isolation Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density gradient centrifugation as previously described (Gupta et al., 201 | Full Paper | |
2018 | Marvin, CM; | Spatial and Temporal Delivery of Small Molecules from Long Wavelength Sensitive Phototherapeutcs Using Erythrocytes as Biological Carriers | Thesis | 525 nm and 660 nm LEDs were purchased from LED Supply. 725 nm and 780 nm LEDs were purchased from Techmark. Erythrocytes were purchased from Allcells. HRMS data was acquired on a Thermo Scientific electrospray linear trap quadrupole fourier transform | Full Paper | |
2018 | Hu, C; | Data-Driven Discovery of Proteomic Hallmarks in Acute Myeloid Leukemia | Thesis | The samples were normalized to a concentration of 1x104 cells/µL and a whole cell lysate was prepared as described in publications [103, 36]. In addition to AML patient samples, each array includes the same ten cryopreserved normal bone marrow CD34+ | Full Paper | |
2018 | Sun, V; | Engineering Solutions for Biomaterials: Self-Assembly and Surface-Modification of Polymers for Clinical Applications | Thesis | Human peripheral blood mononuclear cells (PBMCs) were obtained from AllCells (Alameda, CA). Frozen PBMCs were thawed and cultured in TCM containing X-VIVO™ 15 serum-free medium (Lonza, Allendale NJ), 5% (vol/vol) GemCell human serum antibody AB (Gemi | Full Paper | |
2018 | Weng, H;Huang, H;Wu, H;Qin, X;Zhao, BS;Dong, L;Shi, H;Skibbe, J;Shen, C;Hu, C;Sheng, Y;Wang, Y;Wunderlich, M;Zhang, B;Dore, LC;Su, R;Deng, X;Ferchen, K;Li, C;Sun, M;Lu, Z;Jiang, X;Marcucci, G;Mulloy, JC;Yang, J;Qian, Z;Wei, M;He, C;Chen, J; | METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and Promotes Leukemogenesis via mRNA m6A Modification | Cell stem cell | 29290617 | were then co-transduced with different combinations of retroviruses or lentiviruses as indicated through two rounds of “spinoculation”. Cells were then plated into ColonyGEL methylcellulose medium (ReachBio, Seattle, WA) supplied with 10 ng/ml of mur | Full Paper |
2018 | Probst, K;Stermann, J;von Bomhard, I;Etich, J;Pitzler, L;Niehoff, A;Bluhm, B;Xu, HC;Lang, PA;Chmielewski, M;Abken, H;Blissenbach, B;Machova, A;Papadopoulou, N;Brachvogel, B; | Depletion of Collagen IX Alpha1 Impairs Myeloid Cell Function | Stem cells (Dayton, Ohio) | 30063808 | Flushed bone marrow samples from 2 months old mice were resuspended in Iscove's Modified Dulbecco's Medium containing 10% FCS and used for the colony forming unit assay (ColonyGEL 1203, Reachbio, Seattle) | Full Paper |
2018 | Li, G;Boucher, JC;Kotani, H;Park, K;Zhang, Y;Shrestha, B;Wang, X;Guan, L;Beatty, N;Abate-Daga, D;Davila, ML; | 4-1BB enhancement of CAR T function requires NF-κB and TRAFs | JCI insight | 30232281 | Human peripheral blood mononuclear cells from healthy donors were purchased from ReachBio. Human T cell complete medium consists of RPMI1640 medium, 10% FBS, 2 mM L- Save | Full Paper |
2018 | Kong, J;Han, L;Su, H;Hu, Y;Huang, X;Lou, Y; | Riligustilide Attenuated Renal Injury by the Blockade of Renin | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 30308499 | Mouse anti-CBP monoclonal antibody SC-7300; rabbit anti-CREB polyclonal antibody SC-186 were commercially purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA;). CY5 was obtained from ReachBio WA, USA (#111-165-003), and Alexa Fluor® 488 was | Full Paper |
2018 | Psatha, N;Reik, A;Phelps, S;Zhou, Y;Dalas, D;Yannaki, E;Levasseur, DN;Urnov, FD;Holmes, MC;Papayannopoulou, T; | Disruption of the BCL11A Erythroid Enhancer Reactivates Fetal Hemoglobin in Erythroid Cells of Patients with β-Thalassemia Major | Molecular therapy. Methods & clinical development | 30182035 | anulocyte macrophage [GM] and burst-forming unit-erythroid [BFU-E]), cells were plated at a density of 750-2,000 cells/mL in semi-solid methylcellulose-based medium containing cytokines, ColonyGEL (ReachBio). Zinc Finger Nucleases ZFN pairs were exp | Full Paper |
2018 | Li, C;Psatha, N;Gil, S;Wang, H;Papayannopoulou, T;Lieber, A; | HDAd5/35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells | Molecular therapy. Methods & clinical development | 30038942 | ng/mL SCF, 3 IU/mL EPO, 10 μg/mL insulin, 330 μg/mL transferrin, 2 U/mL heparin, and 1 μg/mL DOX for 6 days. CFU Assay CFU assays with transduced CD34+ cells were performed using ColonyGEL (Reachbio, Seattle, WA, USA) with human complete medium acco | Full Paper |
2018 | Wang, H;Richter, M;Psatha, N;Li, C;Kim, J;Liu, J;Ehrhardt, A;Nilsson, SK;Cao, B;Palmer, D;Ng, P;Izsvák, Z;Haworth, KG;Kiem, HP;Papayannopoulou, T;Lieber, A; | A Combined In Vivo HSC Transduction/Selection Approach Results in Efficient and Stable Gene Expression in Peripheral Blood Cells in Mice | Molecular therapy. Methods & clinical development | 29255741 | Biotec, San Diego, CA, USA) was used in accordance with the manufacturer’s instructions. CFU Assay 1,500 lineage−, GFP+ cells were plated in triplicates in ColonyGEL 1202 mouse complete medium (ReachBio, Seattle, WA, USA) and incubated for 12 days a | Full Paper |
2017 | Lim, JS;Ibaseta, A;Fischer, MM;Cancilla, B;O'Young, G;Cristea, S;Luca, VC;Yang, D;Jahchan, NS;Hamard, C;Antoine, M;Wislez, M;Kong, C;Cain, J;Liu, YW;Kapoun, AM;Garcia, KC;Hoey, T;Murriel, CL;Sage, J; | Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer | Nature | 28489825 | ... Luminex assay for midkine protein in human plasma Human plasma samples from cancer-free normal donors were purchased from BioreclamationIVT. SCLC donor plasma was sourced from Conversant Biologics (Conversant Bio). The samples were collected, p | Full Paper |
2017 | Alley, EW;Lopez, J;Santoro, A;Morosky, A;Saraf, S;Piperdi, B;van Brummelen, E; | Clinical safety and activity of pembrolizumab in patients with malignant pleural mesothelioma (KEYNOTE-028): preliminary results from a non-randomised, open-label, phase 1b trial | Lancet Oncol. | 28291584 | PD-L1 expression was assessed in archival or new tumour samples by a central laboratory (QualTek Molecular Laboratories, Goleta, CA, USA) with a prototype assay and the 22C3 antibody (Merck, Kenilworth, NJ, USA).22, 23 PD-L1 positivity was defined by | Full Paper |
2017 | Plimack, ER;Bellmunt, J;Gupta, S;Berger, R;Chow, LQ;Juco, J;Lunceford, J;Saraf, S;Perini, RF;O'Donnell, PH; | Safety and activity of pembrolizumab in patients with locally advanced or metastatic urothelial cancer (KEYNOTE-012): a non-randomised, open-label, phase 1b study | Lancet Oncol. | 28081914 | Initially, we used a prototype PD-L1 immunohistochemical assay to establish the eligibility of patients in KEYNOTE-012, which was done at a laboratory site at QualTek (Goleta, CA, USA) accredited by the College of American Pathologists and Clinical L | Full Paper |
2017 | Frenel, JS;Le Tourneau, C;O'Neil, B;Ott, PA;Piha-Paul, SA;Gomez-Roca, C;van Brummelen, EMJ;Rugo, HS;Thomas, S;Saraf, S;Rangwala, R;Varga, A; | Safety and Efficacy of Pembrolizumab in Advanced, Programmed Death Ligand 1-Positive Cervical Cancer: Results From the Phase Ib KEYNOTE-028 Trial | J. Clin. Oncol. | 29095678 | tumor sample or newly obtained core or excisional biopsy sample, was defined as membranous staining on $ 1% modified proportion score or interface pattern as assessed using a laboratory-developed prototype immunohistochemistry assay (QualTek Molecula | Full Paper |
2017 | Hsu, C;Lee, SH;Ejadi, S;Even, C;Cohen, RB;Le Tourneau, C;Mehnert, JM;Algazi, A;van Brummelen, EMJ;Saraf, S;Thanigaimani, P;Cheng, JD;Hansen, AR; | Safety and Antitumor Activity of Pembrolizumab in Patients With Programmed Death-Ligand 1-Positive Nasopharyngeal Carcinoma: Results of the KEYNOTE-028 Study | J. Clin. Oncol. | 28837405 | Tumor PD-L1 positivity was established at a central laboratory at baseline on an archived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy sample using a laboratory-developed prototype immunohistochemical assay (QualTek Molec | Full Paper |
2017 | Ott, PA;Elez, E;Hiret, S;Kim, DW;Morosky, A;Saraf, S;Piperdi, B;Mehnert, JM; | Pembrolizumab in Patients With Extensive-Stage Small-Cell Lung Cancer: Results From the Phase Ib KEYNOTE-028 Study | J. Clin. Oncol. | 28813164 | Tumor PD-L1 expression was required to be eligible for treatment in the study; it was evaluated in archival or fresh tumor samples by a central laboratory (QualTek Molecular Laboratories, Goleta, CA) by using a prototype assay and the 22C3 antibody ( | Full Paper |
2017 | Chen, R;Zinzani, PL;Fanale, MA;Armand, P;Johnson, NA;Brice, P;Radford, J;Ribrag, V;Molin, D;Vassilakopoulos, TP;Tomita, A;von Tresckow, B;Shipp, MA;Zhang, Y;Ricart, AD;Balakumaran, A;Moskowitz, CH;, ; | Phase II Study of the Efficacy and Safety of Pembrolizumab for Relapsed/Refractory Classic Hodgkin Lymphoma | J. Clin. Oncol. | 28441111 | PD-L1 expression was determined as previously described, 16 using fresh or archival formalin-fixed, paraffin-embedded pretreatment tissue sectioned at 4 to 5 microns, with a proprietary immunohistochemical assay developed at QualTek Molecular Laborat | Full Paper |
2017 | Colombo, I;Lien, S;Yang, C;Clouthier, D;Bonilla, L;Cyriac, S;Ethier, J;Lee, Y;Kanjanapan, Y;Mandilaras, V;Dhani, N;Butler, M;Oza, A;Quintos, J;Chow, H;Pugh, T;Ohashi, P;Siu, L;Lheureux, S; | Immunologic and genomic characterization of high grade serous ovarian cancer (HGSOC) in patients (pts) treated with pembrolizumab (Pembro) in the phase II INSPIRE trial. | JCO | Mean Tumor Proportion Score of PD-L1 by IHC (Qualtek) was 6.4% (range 0-30%). Grade 3/4 adverse events possibly related to Pembro were observed in 4/18 (22%) pts; none was fatal and the most common were fatigue and hyponatremia | Full Paper | |
2017 | Toy, W;Weir, H;Razavi, P;Lawson, M;Goeppert, AU;Mazzola, AM;Smith, A;Wilson, J;Morrow, C;Wong, WL;De Stanchina, E;Carlson, KE;Martin, TS;Uddin, S;Li, Z;Fanning, S;Katzenellenbogen, JA;Greene, G;Baselga, J;Chandarlapaty, S; | Activating ESR1 Mutations Differentially Affect the Efficacy of ER Antagonists | Cancer Discov | 27986707 | ... n). PATIENT-DERIVED XENOGRAFTS (PDX) CTC-714 PDX model was derived from patient circulating tumor cells (CTC) cultures, consented according to the Human Biological Samples Policy and purchased from Conversant Biologics. The CTCs were obtained f | Full Paper |
2017 | Li, J;Stagg, NJ;Johnston, J;Harris, MJ;Menzies, SA;DiCara, D;Clark, V;Hristopoulos, M;Cook, R;Slaga, D;Nakamura, R;McCarty, L;Sukumaran, S;Luis, E;Ye, Z;Wu, TD;Sumiyoshi, T;Danilenko, D;Lee, GY;Totpal, K;Ellerman, D;Hötzel, I;James, JR;Junttila, TT; | Membrane-Proximal Epitope Facilitates Efficient T Cell Synapse Formation by Anti-FcRH5/CD3 and Is a Requirement for Myeloma Cell Killing | Cancer Cell | 28262555 | ... ec). CD4+ or CD8+ cells were used as effectors in a 3:1 effector:target ratio. In Vitro Cytotoxicity Assay: Human Plasma Cells and Primary MM Samples Human BMMCs from MM patients were procured from Conversant Bio. Human bone marrow aspirates of | Full Paper |
2017 | Le Pichon, CE;Meilandt, WJ;Dominguez, S;Solanoy, H;Lin, H;Ngu, H;Gogineni, A;Sengupta Ghosh, A;Jiang, Z;Lee, SH;Maloney, J;Gandham, VD;Pozniak, CD;Wang, B;Lee, S;Siu, M;Patel, S;Modrusan, Z;Liu, X;Rudhard, Y;Baca, M;Gustafson, A;Kaminker, J;Carano, RAD;Huang, EJ;Foreman, O;Weimer, R;Scearce-Levie, K;Lewcock, JW; | Loss of dual leucine zipper kinase signaling is protective in animal models of neurodegenerative disease | Sci Transl Med | 28814543 | AD (early-stage AD) or confirmed AD along with age-matched controls from Folio Biosciences or Banner Sun Health Research Institute. All human tissues were collected with informed consents and institutional review board approvals. | Full Paper |
2017 | Pearlman, R;Frankel, WL;Swanson, B;Zhao, W;Yilmaz, A;Miller, K;Bacher, J;Bigley, C;Nelsen, L;Goodfellow, PJ;Goldberg, RM;Paskett, E;Shields, PG;Freudenheim, JL;Stanich, PP;Lattimer, I;Arnold, M;Liyanarachchi, S;Kalady, M;Heald, B;Greenwood, C;Paquette, I;Prues, M;Draper, DJ;Lindeman, C;Kuebler, JP;Reynolds, K;Brell, JM;Shaper, AA;Mahesh, S;Buie, N;Weeman, K;Shine, K;Haut, M;Edwards, J;Bastola, S;Wickham, K;Khanduja, KS;Zacks, R;Pritchard, CC;Shirts, BH;Jacobson, A;Allen, B;de la Chapelle, A;Hampel, H;, ; | Prevalence and Spectrum of Germline Cancer Susceptibility Gene Mutations Among Patients With Early-Onset Colorectal Cancer | JAMA Oncol | 27978560 | Myriad Genetics Inc donated the next-generation sequencing testing for mismatch repair–proficient patients with CRC diagnosed younger than 50 years for this study. Dr Kalady discloses honorarium from Helomics and Transenterix and is a member of the s | Full Paper |
2017 | Ott, PA;Piha-Paul, SA;Munster, P;Pishvaian, MJ;van Brummelen, EMJ;Cohen, RB;Gomez-Roca, C;Ejadi, S;Stein, M;Chan, E;Simonelli, M;Morosky, A;Saraf, S;Emancipator, K;Koshiji, M;Bennouna, J; | Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with recurrent carcinoma of the anal canal | Ann. Oncol. | 28453692 | mbedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek Molecular Laboratories, Goleta, CA) [15] u | Full Paper |
2017 | Hui, R;Garon, EB;Goldman, JW;Leighl, NB;Hellmann, MD;Patnaik, A;Gandhi, L;Eder, JP;Ahn, MJ;Horn, L;Felip, E;Carcereny, E;Rangwala, R;Lubiniecki, GM;Zhang, J;Emancipator, K;Roach, C;Rizvi, NA; | Pembrolizumab as first-line therapy for patients with PD-L1-positive advanced non-small cell lung cancer: a phase 1 trial | Ann. Oncol. | 28168303 | As previously described [5], PD-L1 expression for enrollment was assessed with a prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA) and the relationship between PD-L1 expression and efficacy was investigated using a cli | Full Paper |
2017 | Potts, AH;Vakulskas, CA;Pannuri, A;Yakhnin, H;Babitzke, P;Romeo, T; | Global role of the bacterial post-transcriptional regulator CsrA revealed by integrated transcriptomics | Nat Commun | 29150605 | ;... nufacturer’s instructions with one modification. Adaptors and reverse transcription primers were diluted 1 in 5 in water before use. Libraries were sequenced using HiSeq 2500 50SE (Illumina) by the HudsonAlpha Genomic Services Laboratory. Seque | Full Paper |
2017 | Christopher, MA;Myrick, DA;Barwick, BG;Engstrom, AK;Porter-Stransky, KA;Boss, JM;Weinshenker, D;Levey, AI;Katz, DJ; | LSD1 protects against hippocampal and cortical neurodegeneration | Nat Commun | 28993646 | ... s possible, then RNA was precipitated with isopropanol. Pellets were then washed with 75% ethanol and resuspended in 50 µL deionized water. RNA library preparation and sequencing were performed by HudsonAlpha Genomic Services Lab. RNA was Poly(A) | Full Paper |
2017 | Ramaker, RC;Savic, D;Hardigan, AA;Newberry, K;Cooper, GM;Myers, RM;Cooper, SJ; | A genome-wide interactome of DNA-associated proteins in the human liver | Genome Res. | 29021291 | HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for Bi | Full Paper |
2017 | Cubeñas-Potts, C;Rowley, MJ;Lyu, X;Li, G;Lei, EP;Corces, VG; | Different enhancer classes in Drosophila bind distinct architectural proteins and mediate unique chromatin interactions and 3D architecture | Nucleic Acids Res. | 27899590 | New ChIP experiments were performed in Kc167 cells for GAF, H3, H3K27me3, Ibf1, Ibf2, Pita, Nup98 and ZIPIC as described previously with minor modifications (28). More precisely, antibody-bound protein complexes were isolated with Protein A or Protei | Full Paper |
2017 | Edwardson, CF;Hollibaugh, JT; | Metatranscriptomic analysis of prokaryotic communities active in sulfur and arsenic cycling in Mono Lake, California, USA | ISME J | 28548659 | synthesis kits. Libraries (~225 bp insert) were prepared using Illumina TruSeq technology. Samples were pooled and run on one lane of 150 × 2 Illumina HiSeq 2500 Rapid Run at HudsonAlpha Genomic Services. Reads have | Full Paper |
2017 | Yeh, TC;O'Connor, G;Petteruti, P;Dulak, A;Hattersley, M;Barrett, JC;Chen, H; | Identification of CCR2 and CD180 as Robust Pharmacodynamic Tumor and Blood Biomarkers for Clinical Use with BRD4/BET Inhibitors | Clin. Cancer Res. | 28073847 | Whole blood was collected in 10 mL EDTA vaccutainers (Becton Dickinson) from two control healthy volunteers and 11 patients with cancer with solid tumor or hematologic cancers through Conversant Bio. Compound incubation and sample processing was carr | Full Paper |
2017 | Kirtane, AR;Sadhukha, T;Kim, H;Khanna, V;Koniar, B;Panyam, J; | Fibrinolytic Enzyme Cotherapy Improves Tumor Perfusion and Therapeutic Efficacy of Anticancer Nanomedicine | Cancer Res. | 28108516 | ... anticancer nanomedicine in syngeneic mouse melanoma and xenograft lung cancer models. Go to: MATERIALS AND METHODS MATERIALS Tissue microarrays of various human tumor biopsies were obtained from Folio Biosciences (Ohio, USA). Anti-human fibr | Full Paper |
2017 | Jung, YH;Sauria, MEG;Lyu, X;Cheema, MS;Ausio, J;Taylor, J;Corces, VG; | Chromatin States in Mouse Sperm Correlate with Embryonic and Adult Regulatory Landscapes | Cell Rep | 28178516 | We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology, and specially Drs. Braden Boone, Angela Jones and Terri Pointer, for their help in performing Illumina sequencing of samples. This work was supported by U | Full Paper |
2017 | O'Daniel, JM;McLaughlin, HM;Amendola, LM;Bale, SJ;Berg, JS;Bick, D;Bowling, KM;Chao, EC;Chung, WK;Conlin, LK;Cooper, GM;Das, S;Deignan, JL;Dorschner, MO;Evans, JP;Ghazani, AA;Goddard, KA;Gornick, M;Farwell Hagman, KD;Hambuch, T;Hegde, M;Hindorff, LA;Holm, IA;Jarvik, GP;Knight Johnson, A;Mighion, L;Morra, M;Plon, SE;Punj, S;Richards, CS;Santani, A;Shirts, BH;Spinner, NB;Tang, S;Weck, KE;Wolf, SM;Yang, Y;Rehm, HL; | A survey of current practices for genomic sequencing test interpretation and reporting processes in US laboratories | Genet. Med. | 27811861 | Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.++Laboratory for Molecular Medicine, Partners Healthcare Personalized Medicine, Cambridge, Massachusetts, USA.++Division of Medical Genetics, Univer | Full Paper |
2017 | Rydzak, T;Garcia, D;Stevenson, DM;Sladek, M;Klingeman, DM;Holwerda, EK;Amador-Noguez, D;Brown, SD;Guss, AM; | Deletion of Type I glutamine synthetase deregulates nitrogen metabolism and increases ethanol production in Clostridium thermocellum | Metab. Eng. | 28400329 | ; diluted. Sequencing was completed using a SR50 sequencing protocol and V4 chemistry on an Illumina HiSeq. 2500 platform (HudsonAlpha Genomic Services Laboratory; Huntsville, AL). 2.7. RNA-Seq analysis. Raw reads | Full Paper |
2017 | Tigue, NJ;Bamber, L;Andrews, J;Ireland, S;Hair, J;Carter, E;Sridharan, S;Jovanović, J;Rees, DG;Springall, JS;Solier, E;Li, YM;Chodorge, M;Perez-Martinez, D;Higazi, DR;Oberst, M;Kennedy, M;Black, CM;Yan, L;Schwickart, M;Maguire, S;Cann, JA;de Haan, L;Young, LL;Vaughan, T;Wilkinson, RW;Stewart, R; | MEDI1873, a potent, stabilized hexameric agonist of human GITR with regulatory T-cell targeting potential | Oncoimmunology | 28405505 | ... xP3 Tissue samples from 76 cancer patients, with NSCLC, colorectal cancer (CRC), bladder cancer, or squamous cell cancer of the head and neck (SCCHN), were obtained from commercial sources (Asterand, ConversantBio, ProteoGenex, National Disease R | Full Paper |
2017 | Liu, Z;Zhang, C;Skamagki, M;Khodadadi-Jamayran, A;Zhang, W;Kong, D;Chang, CW;Feng, J;Han, X;Townes, TM;Li, H;Kim, K;Zhao, R; | Elevated p53 Activities Restrict Differentiation Potential of MicroRNA-Deficient Pluripotent Stem Cells | Stem Cell Reports | 29141234 | Total RNA was extracted from Dgcr8−/−-302 cells and submitted to the Genomic Services Lab at the HudsonAlpha Institute. The library was constructed by the standard miRNA library construction protocol (Illumina) and 15 million, 50 bp single-end reads | Full Paper |
2017 | Bowling, KM;Thompson, ML;Amaral, MD;Finnila, CR;Hiatt, SM;Engel, KL;Cochran, JN;Brothers, KB;East, KM;Gray, DE;Kelley, WV;Lamb, NE;Lose, EJ;Rich, CA;Simmons, S;Whittle, JS;Weaver, BT;Nesmith, AS;Myers, RM;Barsh, GS;Bebin, EM;Cooper, GM; | Genomic diagnosis for children with intellectual disability and/or developmental delay | Genome Med | 28554332 | Methods" (see Additional file 1). Whole-exome and whole-genome sequencing. Blood samples were sent for sequencing at the HudsonAlpha Genomic Services Laboratory (http://gsl.hudsonalpha.org). Genomic DNA was isolated | Full Paper |
2017 | Harden, ME;Prasad, N;Griffiths, A;Munger, K; | Modulation of microRNA-mRNA Target Pairs by Human Papillomavirus 16 Oncoproteins | MBio | 28049151 | ;... million 50-bp PE reads) was performed with the Illumina MiSeq Sequencing System (Illumina). miR-seq data analysis. Postprocessing of the miR-seq reads from each sample was performed according to the HudsonAlpha Genomic Services Laboratory (GSL) | Full Paper |
2017 | Weiss, G;Schlegel, A;Kottwitz, D;König, T;Tetzner, R; | Validation of the SHOX2/PTGER4 DNA Methylation Marker Panel for Plasma-Based Discrimination between Patients with Malignant and Nonmalignant Lung Disease | J Thorac Oncol | 27544059 | The patient samples were either material collected over the course of several months by commercial partners (ProteoGenex Inc., Sofia Bio LLC) or leftover material from previously conducted clinical trials in colon cancer.21, 22 More specifically, pat | Full Paper |
2017 | Steffen, MM;Davis, TW;McKay, RML;Bullerjahn, GS;Krausfeldt, LE;Stough, JMA;Neitzey, ML;Gilbert, NE;Boyer, GL;Johengen, TH;Gossiaux, DC;Burtner, AM;Palladino, D;Rowe, MD;Dick, GJ;Meyer, KA;Levy, S;Boone, BE;Stumpf, RP;Wynne, TT;Zimba, PV;Gutierrez, D;Wilhelm, SW; | Ecophysiological Examination of the Lake Erie Microcystis Bloom in 2014: Linkages between Biology and the Water Supply Shutdown of Toledo, OH | Environ. Sci. Technol. | 28535339 | RNA was stored at −80 °C until sent to HudsonAlpha Institute for Biotechnology (Huntsville, AL) for sequencing. Total RNA concentrations and quality were assessed fluorometrically via RiboGreen (Life Technologies, Carlsbad, CA) followed by integrity | Full Paper |
2017 | Park, KC;Lee, M;Jeon, Y;Jeon, R;Baek, SH;Lee, H;Kim, KI; | Skin-Specific Deletion of Mis18α Impedes Proliferation and Stratification of Epidermal Keratinocytes | J. Invest. Dermatol. | 27670610 | Materials and Methods. In situ hybridization. All procedures of in situ hybridization, except probe design and cloning, were performed by Phylogeny Inc. (Columbus, OH). Briefly, the whole bodies of C57BL/6 mice were frozen and cut into 10-μm sections | Full Paper |
2017 | Blanchet, P;Bebin, M;Bruet, S;Cooper, GM;Thompson, ML;Duban-Bedu, B;Gerard, B;Piton, A;Suckno, S;Deshpande, C;Clowes, V;Vogt, J;Turnpenny, P;Williamson, MP;Alembik, Y;, ;, ;Glasgow, E;McNeill, A; | MYT1L mutations cause intellectual disability and variable obesity by dysregulating gene expression and development of the neuroendocrine hypothalamus | PLoS Genet. | 28859103 | Département de Génétique Médicale, Hôpital Arnaud de Villeneuve, Montpellier Cedex 5, France.++Department of Neurology, University of Alabama at Birmingham, Birmingham, AL, United States of America.++Service de génétique médicale, Hôpitaux Universita | Full Paper |
2017 | Mojiri, A;Stoletov, K;Carrillo, MA;Willetts, L;Jain, S;Godbout, R;Jurasz, P;Sergi, CM;Eisenstat, DD;Lewis, JD;Jahroudi, N; | Functional assessment of von Willebrand factor expression by cancer cells of non-endothelial origin | Oncotarget | 28035064 | Immunofluorescent staining of cultured cells were performed as previously described [34]. Immunofluorescent staining of slides containing sectioned paraffin imbedded biopsies of normal human brain and glioma (Dr. D. Eisenstat from the Brain Tumor Tis | Full Paper |
2017 | McDaniel, JM;Varley, KE;Gertz, J;Savic, DS;Roberts, BS;Bailey, SK;Shevde, LA;Ramaker, RC;Lasseigne, BN;Kirby, MK;Newberry, KM;Partridge, EC;Jones, AL;Boone, B;Levy, SE;Oliver, PG;Sexton, KC;Grizzle, WE;Forero, A;Buchsbaum, DJ;Cooper, SJ;Myers, RM; | Genomic regulation of invasion by STAT3 in triple negative breast cancer | Oncotarget | 28030809 | ... ell viability and invasion assays. All ChIP-seq and RNA-seq datasets have been deposited in GEO (accession number: GSE85579). SUPPLEMENTARY MATERIALS FIGURES AND TABLES We thank the members of the HudsonAlpha Genomic Services Laboratory for pr | Full Paper |
2017 | Ranganathan, P;Ngankeu, A;Zitzer, NC;Leoncini, P;Yu, X;Casadei, L;Challagundla, K;Reichenbach, DK;Garman, S;Ruppert, AS;Volinia, S;Hofstetter, J;Efebera, YA;Devine, SM;Blazar, BR;Fabbri, M;Garzon, R; | Serum miR-29a Is Upregulated in Acute Graft-versus-Host Disease and Activates Dendritic Cells through TLR Binding | J. Immunol. | 28159900 | ... Go to: ACKNOWLEDGMENTS We thank Carlo M Croce at The Ohio State University for providing us with miR29a KO mice. We thank Jordan Smith for assisting in collection and processing of blood samples. Phylogeny Inc., Columbus, Ohio, performed LNA- | Full Paper |
2017 | Xu-Monette, ZY;Zhang, M;Li, J;Young, KH; | PD-1/PD-L1 Blockade: Have We Found the Key to Unleash the Antitumor Immune Response? | Front Immunol | 29255458 | 99.5%; 75.6% of patients had a response for ≥6 months Clinical activity was seen across all PD-L1 groups defined by PD-L1 intensity score, tumor-membrane staining score, and histiocyte score (QualTek IHC assay); 90.4% of patients had an intensity | Full Paper |
2017 | Zeng, Q;Tian, X;Wang, L; | Genetic adaptation of microbial populations present in high-intensity catfish production systems with therapeutic oxytetracycline treatment | Sci Rep | 29235508 | ;... DNA (~1000 ng/sample) extracted from every sediment sample collected from the treated or the control system, were pooled for sequencing. The library construction and sequencing were conducted at the HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2017 | Oliveira, ERA;Póvoa, TF;Nuovo, GJ;Allonso, D;Salomão, NG;Basílio-de-Oliveira, CA;Geraldo, LHM;Fonseca, CG;Lima, FRS;Mohana-Borges, R;Paes, MV; | Dengue fatal cases present virus-specific HMGB1 response in peripheral organs | Sci Rep | 29167501 | ... iro, Brazil 2Instituto de Criminalística, Tocantins, Brazil 30000 0001 2285 7943grid.261331.4Ohio State University Comprehensive Cancer Center, Columbus, Ohio, United States of America 4grid.430298.4Phylogeny Inc, Powell, Ohio, United States of A | Full Paper |
2017 | Zhang, Y;Saavedra, E;Tang, R;Gu, Y;Lappin, P;Trajkovic, D;Liu, SH;Smeal, T;Fantin, V;De Botton, S;Legrand, O;Delhommeau, F;Pernasetti, F;Louache, F; | Targeting primary acute myeloid leukemia with a new CXCR4 antagonist IgG1 antibody (PF-06747143) | Sci Rep | 28779088 | ... ratio and 1:50 ratios, respectively (n = 4/group). ToxiLight bioluminescent cytotoxicity assay (Lonza) was used to detect cell lysis. The AML patient primary bone marrow sample was obtained from Conversant Bio (sample ID #110032873). Mice NSG m | Full Paper |
2017 | Aumiller, V;Strobel, B;Romeike, M;Schuler, M;Stierstorfer, BE;Kreuz, S; | Comparative analysis of lysyl oxidase (like) family members in pulmonary fibrosis | Sci Rep | 28273952 | Normal and fibrotic lung tissue samples from the BLM-induced mouse model were prepared and Masson trichrome stained as described previously27. Human IPF and healthy lung tissue was purchased at Folio bioscience (Columbus, Ohio, USA) following the gui | Full Paper |
2017 | Jane, EP;Premkumar, DR;Sutera, PA;Cavaleri, JM;Pollack, IF; | Survivin inhibitor YM155 induces mitochondrial dysfunction, autophagy, DNA damage and apoptosis in Bcl-xL silenced glioma cell lines | Mol. Carcinog. | 27805285 | collected at the time of tumor resection. We also obtained primary GBM cells from Conversant Biologics (Huntsville, AL), which were cultured in DMEM/F12 (Invitrogen, Carlsbad, CA). GIBCO® Human Astrocytes, normal human cells | Full Paper |
2017 | Appleby, TC;Greenstein, AE;Hung, M;Liclican, A;Velasquez, M;Villaseñor, AG;Wang, R;Wong, MH;Liu, X;Papalia, GA;Schultz, BE;Sakowicz, R;Smith, V;Kwon, HJ; | Biochemical characterization and structure determination of a potent, selective antibody inhibitor of human MMP9 | J. Biol. Chem. | 28235803 | Ulcerative colitis tissue was obtained after surgical colectomy (Folio Biosciences). Specimens were selected based on disease severity scored by a pathologist on matched FFPE tissues. Frozen fragments of ~50 mg were lysed in 10 mM Hepes pH 7.5, 150 m | Full Paper |
2017 | Cho, JH;Sorensen, SF;Choi, YL;Feng, Y;Kim, TE;Choi, H;Georgsen, JB;Dolled-Filhart, M;Emancipator, K;Meldgaard, P;Sun, JM;Kim, HK;Choi, YS;Shim, YM;Zhou, W;Hager, H;Kim, J; | Programmed Death Ligand 1 Expression in Paired Non-Small Cell Lung Cancer Tumor Samples | Clin Lung Cancer | 28669849 | charged slides. The TechMate 500 instrument (QualTek, Goleta, CA) was used with the 22C3 antibody for automated staining. A membrane staining. All of the PD-L1 IHC assays were done at QualTek, as the central laboratory. PD | Full Paper |
2017 | Sundahl, N;De Wolf, K;Rottey, S;Decaestecker, K;De Maeseneer, D;Meireson, A;Goetghebeur, E;Fonteyne, V;Verbeke, S;De Visschere, P;Reynders, D;Van Gele, M;Brochez, L;Ost, P; | A phase I/II trial of fixed-dose stereotactic body radiotherapy with sequential or concurrent pembrolizumab in metastatic urothelial carcinoma: evaluation of safety and clinical and immunologic response | J Transl Med | 28662677 | Exploratory endpoints. Response rate according to expression of PD-L1. PD-L1 expression will be determined by immunohistochemistry in QualTek Molecular Laboratories on an archival or newly obtained tissue sample of the tumor or metastatic lesion | Full Paper |
2017 | Lagatie, O;Van Dorst, B;Stuyver, LJ; | Identification of three immunodominant motifs with atypical isotype profile scattered over the Onchocerca volvulus proteome | PLoS Negl Trop Dis | 28125577 | For determination of specificity of the peptide ELISA’s, different sets of plasma samples were obtained from Discovery Life Sciences, Inc. (Los Osos, USA), Bioreclamation IVT (Baltimore, USA), FR3 or Mayo Clinic (Jacksonville, USA). Written informed | Full Paper |
2017 | Pellegrini, KL;Patil, D;Douglas, KJS;Lee, G;Wehrmeyer, K;Torlak, M;Clark, J;Cooper, CS;Moreno, CS;Sanda, MG; | Detection of prostate cancer-specific transcripts in extracellular vesicles isolated from post-DRE urine | Prostate | 28419548 | ; targeted in this analysis). The libraries were sequenced at the HudsonAlpha Genomic Services Lab to produce 150 bases of paired‐end reads with an average of over 300 000 reads mapped per sample. The 150 base sequence | Full Paper |
2017 | Wilson, CM;Klingeman, DM;Schlachter, C;Syed, MH;Wu, CW;Guss, AM;Brown, SD; | LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313 | Appl. Environ. Microbiol. | 28003194 | ;... Samples were pooled into three groups to make a stock of 20 nM in 72 μl. A 40-μl aliquot of the three pooled libraries was sequenced using an SR50 sequencing run on an Illumina HiSeq 2500 platform (HudsonAlpha Genomic Services Laboratory, Huntsv | Full Paper |
2017 | Zhang, Y;Song, J;Day, K;Absher, D; | dCATCH-Seq: improved sequencing of large continuous genomic targets with double-hybridization | BMC Genomics | 29061109 | HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA. dabsher@hudsona | Full Paper |
2017 | Matsunaga, Y;Hwang, H;Franke, B;Williams, R;Penley, M;Qadota, H;Yi, H;Morran, LT;Lu, H;Mayans, O;Benian, GM; | Twitchin kinase inhibits muscle activity | Mol. Biol. Cell | 28428253 | Genomic DNA from unc-22(sf21) was prepared and provided to the Emory Integrated Genomics Core (EIGC) for Illumina sequencing. The Genomic Services Laboratory at the HudsonAlpha Institute for Biotechnology performed quality control and constructed sta | Full Paper |
2017 | Liu, Z;Zhang, C;Khodadadi-Jamayran, A;Dang, L;Han, X;Kim, K;Li, H;Zhao, R; | Canonical microRNAs Enable Differentiation, Protect Against DNA Damage, and Promote Cholesterol Biosynthesis in Neural Stem Cells | Stem Cells Dev. | 27762676 | For mRNA-seq and small RNA-seq analyses, total RNA samples prepared from Dgcr8+/− and Dgcr8−/− NSCs were submitted to the Genomic Services Laboratory at HudsonAlpha Institute (Huntsville, AL) for library construction and sequencing. For mRNA-seq anal | Full Paper |
2017 | Aycock, KI;Campbell, RL;Lynch, FC;Manning, KB;Craven, BA; | Computational predictions of the embolus-trapping performance of an IVC filter in patient-specific and idealized IVC geometries | Biomech Model Mechanobiol | 28656515 | Full Paper | |
2017 | George, AF;Rahman, KM;Camp, ME;Prasad, N;Bartol, FF;Bagnell, CA; | Defining age- and lactocrine-sensitive elements of the neonatal porcine uterine microRNA-mRNA interactome | Biol. Reprod. | 28203709 | Department of Animal Sciences, Endocrinology, and Animal Biosciences Program, Rutgers University, New Brunswick, New Jersey, USA.++Department of Animal Sciences, Endocrinology, and Animal Biosciences Program, Rutgers University, New Brunswick, New Je | Full Paper |
2017 | Kirby, MK;Ramaker, RC;Roberts, BS;Lasseigne, BN;Gunther, DS;Burwell, TC;Davis, NS;Gulzar, ZG;Absher, DM;Cooper, SJ;Brooks, JD;Myers, RM; | Genome-wide DNA methylation measurements in prostate tissues uncovers novel prostate cancer diagnostic biomarkers and transcription factor binding patterns | BMC Cancer | 28412973 | ;... ng characteristic RRBS Reduced representation bisulfite sequencing TCGA The cancer genome atlas TF Transcription factor TMPRSS2 Transmembrane protease, serine 2 Acknowledgements We thank the HudsonAlpha Genomic Services Lab for providin | Full Paper |
2017 | Costa-Paiva, EM;Whelan, NV;Waits, DS;Santos, SR;Schrago, CG;Halanych, KM; | Discovery and evolution of novel hemerythrin genes in annelid worms | BMC Evol. Biol. | 28330441 | Despite extensive study on hemoglobins and hemocyanins, little is known about hemerythrin (Hr) evolutionary history. Four subgroups of Hrs have been documented, including: circulating Hr (cHr), myohemerythrin (myoHr), ovohemerythrin (ovoHr), and neur | Full Paper |
2017 | Pizzi, M;Inghirami, G; | Patient-derived tumor xenografts of lymphoproliferative disorders: are they surrogates for the human disease? | Curr. Opin. Hematol. | 28594662 | Patient-derived xenograft models WuXi AppTec http://www.urolead.com Xenografts Conversant Bio http://www.conversantbio.com/pricing Xenograft models ONCOdesign http://www.oncodesign. com/en/contact-us Humanized mice HuMurine http://humurine.com | Full Paper |
2017 | Zhang, D;Thongda, W;Li, C;Zhao, H;Beck, BH;Mohammed, H;Arias, CR;Peatman, E; | More than just antibodies: Protective mechanisms of a mucosal vaccine against fish pathogen Flavobacterium columnare | Fish Shellfish Immunol. | 28989091 | ; 2000 instrument (Thermo Scientific, Wilmington, DE). RNA-seq library preparation and sequencing were carried out by the HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). Briefly, TruSeq RNA Sample Preparation | Full Paper |
2017 | Zhu, J;Fu, Q;Ao, Q;Tan, Y;Luo, Y;Jiang, H;Li, C;Gan, X; | Transcriptomic profiling analysis of tilapia (Oreochromis niloticus) following Streptococcus agalactiae challenge | Fish Shellfish Immunol. | 28111359 | ; For each timepoint, equal amount of RNA from individual fish were pooled together (15 fish) for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out in HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) | Full Paper |
2017 | McCoy, CR;Jackson, NL;Day, J;Clinton, SM; | Genetic predisposition to high anxiety- and depression-like behavior coincides with diminished DNA methylation in the adult rat amygdala | Behav. Brain Res. | 27965039 | ; Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) for next-generation sequencing using NEBNext reagents (New England Biolabs, Ipswich, MA) according to manufacturer's recommendations | Full Paper |
2017 | Stebbins, KJ;Broadhead, AR;Cabrera, G;Correa, LD;Messmer, D;Bundey, R;Baccei, C;Bravo, Y;Chen, A;Stock, NS;Prasit, P;Lorrain, DS; | In vitro and in vivo pharmacology of NXT629, a novel and selective PPARα antagonist | Eur. J. Pharmacol. | 28483457 | expression of PPARα, normal healthy or melanoma skin biopsy samples (n=2 donors each) were obtained from ConversantBio (Hunstville, Alabama) Normal (n=5) and melanoma (n=4) human skin biopsy samples were obtained from Conversant Bio and expression | Full Paper |
2017 | Pirie-Shepherd, SR;Painter, C;Whalen, P;Vizcarra, P;Roy, M;Qian, J;Franks, T;Coskran, T;Golas, J;Deng, S;Zhong, W;Tucker, E;Marrinucci, D;Gerber, HP;Powell, EL; | Detecting expression of 5T4 in CTCs and tumor samples from NSCLC patients | PLoS ONE | 28727782 | ... d from a variety of sources, including the Pfizer tumor bank and commercial vendors. All commercially sourced human biospecimens, including blood samples and matched tumor tissues, were obtained from Conversant Bio. Biospecimens distributed by Co | Full Paper |
2017 | Awasthi Mishra, N;Drögemüller, C;Jagannathan, V;Keller, I;Wüthrich, D;Bruggmann, R;Beck, J;Schütz, E;Brenig, B;Demmel, S;Moser, S;Signer-Hasler, H;Pieńkowska-Schelling, A;Schelling, C;Sande, M;Rongen, R;Rieder, S;Kelsh, RN;Mercader, N;Leeb, T; | A structural variant in the 5'-flanking region of the TWIST2 gene affects melanocyte development in belted cattle | PLoS ONE | 28658273 | Center Zurich, and the Genomic Services Lab at HudsonAlpha for performing experiments. Computationally intensive tasks were performed at the the Interfaculty Bioinformatics Unit of the University of Bern and the Vital-IT high-performance computing ce | Full Paper |
2017 | Carlson, DE;Hedin, M; | Comparative transcriptomics of Entelegyne spiders (Araneae, Entelegynae), with emphasis on molecular evolution of orphan genes | PLoS ONE | 28379977 | Next-generation sequencing technology is rapidly transforming the landscape of evolutionary biology, and has become a cost-effective and efficient means of collecting exome information for non-model organisms. Due to their taxonomic diversity, produc | Full Paper |
2017 | Howard, LM;Hoek, KL;Goll, JB;Samir, P;Galassie, A;Allos, TM;Niu, X;Gordy, LE;Creech, CB;Prasad, N;Jensen, TL;Hill, H;Levy, SE;Joyce, S;Link, AJ;Edwards, KM; | Cell-Based Systems Biology Analysis of Human AS03-Adjuvanted H5N1 Avian Influenza Vaccine Responses: A Phase I Randomized Controlled Trial | PLoS ONE | 28099485 | Vanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, United States of America.++Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashvil | Full Paper |
2017 | O'Neil, BH;Wallmark, JM;Lorente, D;Elez, E;Raimbourg, J;Gomez-Roca, C;Ejadi, S;Piha-Paul, SA;Stein, MN;Abdul Razak, AR;Dotti, K;Santoro, A;Cohen, RB;Gould, M;Saraf, S;Stein, K;Han, SW; | Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with advanced colorectal carcinoma | PLoS ONE | 29284010 | mbedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek Molecular Laboratories, Goleta, CA, USA) [ | Full Paper |
2017 | Danaee, H;Kalebic, T;Wyant, T;Fassan, M;Mescoli, C;Gao, F;Trepicchio, WL;Rugge, M; | Consistent expression of guanylyl cyclase-C in primary and metastatic gastrointestinal cancers | PLoS ONE | 29261789 | GCC IHC analysis and pathology assessment. GCC staining was performed on the Techmate Automated IHC Platform (BioTek Solutions/Ventana Medical Systems, Tucson, AZ, USA; QualTek Laboratories, Goleta, CA, USA) ;two independent pathologists. GCC I | Full Paper |
2017 | O'Neil, BH;Wallmark, JM;Lorente, D;Elez, E; | Safety and antitumor activity of the anti–PD-1 antibody pembrolizumab in patients with advanced colorectal carcinoma | PloS one | archived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory- developed prototype immunohistochemistry (IHC) assay (QualTek Molecu | Full Paper | |
2017 | Eboigbodin, KE;Moilanen, K;Elf, S;Hoser, M; | Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA® | BMC Infect. Dis. | 28183291 | isothermal conditions. Methods. Clinical specimens and rapid processing. A total of 40 nasopharyngeal (NP) swab specimens were obtained from Discovery Life Sciences Biobank (Discovery Life Sciences, Inc., CA, USA). The | Full Paper |
2017 | Liu, DT;Brewer, MS;Chen, S;Hong, W;Zhu, Y; | Transcriptomic signatures for ovulation in vertebrates | Gen. Comp. Endocrinol. | 28111234 | PCR (qPCR) assay. 2.4. Library construction and Illumina sequencing. RNA-Seq library preparation and high-throughput NGS sequencing were carried out at HudsonAlpha Genomic Services Lab (Huntsville, AL). Qubit® 3.0 | Full Paper |
2017 | Bhayana, S;Song, F;Jacob, J;Fadda, P;Denko, NC;Xu-Welliver, M;Chakravarti, A;Jacob, NK; | Urinary miRNAs as Biomarkers for Noninvasive Evaluation of Radiation-Induced Renal Tubular Injury | Radiat. Res. | 28977780 | W81XWH-15-2-0054). We thank Dr. Jerry Nuovo of Phylogeny, Inc. (Powell, OH) for technical assistance. REFERENCES. 1. Hingorani S. Chronic kidney disease in long-term survivors of hematopoietic cell transplantation: epidemiology, pathogenesis, and tre | Full Paper |
2017 | Kadić, E;Moniz, RJ;Huo, Y;Chi, A;Kariv, I; | Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determinated by multiparametric single cell mass cytometry analysis | BMC Immunol. | 28148223 | ... ry detection antibodies Sample sourcing PBMCs were purchased from SeraCare Life Sciences Inc., (Milford, MA). Frozen dissociated tumor cells (DTC) and normal adjacent tissue (NAT) were purchased from ConversantBio (Huntsville, AL). The frozen sam | Full Paper |
2017 | Parlato, M;Molenda, J;Murphy, WL; | Specific recruitment of circulating angiogenic cells using biomaterials as filters | Acta biomaterialia | 28373084 | CD133+ cells collected from mobilized peripheral blood were purchased from AllCells, LLC. These cells were collected by AllCells, LLC from one donor (26 year old, African American, Save | Full Paper |
2017 | Block, TS;Murphy, TI;Munster, PN;Nguyen, DP;Lynch, FJ; | Glucocorticoid receptor expression in 20 solid tumor types using immunohistochemistry assay | Cancer Manag Res | 28293120 | Tiffany I Murphy. 3 QualTek Molecular Laboratories, Newtown, PA. Find articles by Tiffany I Murphy. Pamela N Munster Dat P Nguyen. 1 Corcept Therapeutics, Inc, Menlo Park, CA. Find articles by Dat P Nguyen. Frank J Lynch. 3 QualTek Molecular Labora | Full Paper |
2017 | Coletti, M;Hultquist, C;Kennedy, WG;Cervone, G; | Validating Safecast data by comparisons to a U. S. Department of Energy Fukushima Prefecture aerial survey | J Environ Radioact | 28167372 | Second, units are randomly selected, assembled, and undergo calibration tests at the Julich Research Centre in Germany, QualTek in the US, and the International Atomic Energy Agency (IAEA) testing laboratory in Seibersdorf, Austria | Full Paper |
2017 | Thabet, AA;Maas, AE;Saber, SA;Tarrant, AM; | Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposure | Mar Genomics | 28385518 | Total RNA was extracted from pools of C. limacina juveniles using the Aurum Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad). RNA purity and integrity were quantified using a Nanodrop ND-1000 spectrophotometer, and quality was assessed using a Bioana | Full Paper |
2017 | Matsushita, M;Ozawa, K;Suzuki, T;Nakamura, M;Nakano, N;Kanchi, S;Ichikawa, D;Matsuki, E;Sakurai, M;Karigane, D;Kasahara, H;Tsukamoto, N;Shimizu, T;Mori, T;Nakajima, H;Okamoto, S;Kawakami, Y;Hattori, Y; | CXorf48 is a potential therapeutic target for achieving treatment-free remission in CML patients | Blood cancer journal | 28862699 | ty gradient centrifugation and cryopreserved in Cell Banker reagent (Juji-Field, Tokyo, Japan) in liquid nitrogen until further use. Bone marrow mononuclear cells (BMMNC) from HDs were purchased from AllCells, LLC. (Alameda, CA, USA). This study was | Full Paper |
2017 | Simeonov, DR;Gowen, BG;Boontanrart, M;Roth, TL;Gagnon, JD;Mumbach, MR;Satpathy, AT;Lee, Y;Bray, NL;Chan, AY;Lituiev, DS;Nguyen, ML;Gate, RE;Subramaniam, M;Li, Z;Woo, JM;Mitros, T;Ray, GJ;Curie, GL;Naddaf, N;Chu, JS;Ma, H;Boyer, E;Van Gool, F;Huang, H;Liu, R;Tobin, VR;Schumann, K;Daly, MJ;Farh, KK;Ansel, KM;Ye, CJ;Greenleaf, WJ;Anderson, MS;Bluestone, JA;Chang, HY;Corn, JE;Marson, A; | Discovery of stimulation-responsive immune enhancers with CRISPR activation | Nature | 28854172 | Normal donor human peripheral blood cells were obtained fresh from AllCells. CD4 + T cells were enriched from peripheral blood using the RosetteSep Human CD4 + T Cell Save | Full Paper |
2017 | Kawamura, S;Onai, N;Miya, F;Sato, T;Tsunoda, T;Kurabayashi, K;Yotsumoto, S;Kuroda, S;Takenaka, K;Akashi, K;Ohteki, T; | Identification of a Human Clonogenic Progenitor with Strict Monocyte Differentiation Potential: A Counterpart of Mouse cMoPs | Immunity | 28514689 | Human UCB samples were provided by the Japanese Red Cross Kanto-Koshinetsu Cord Blood Bank, and human BM samples were purchased from AllCells. All experiments were Save | Full Paper |
2017 | Peterson, VM;Zhang, KX;Kumar, N;Wong, J;Li, L;Wilson, DC;Moore, R;McClanahan, TK;Sadekova, S;Klappenbach, JA; | Multiplexed quantification of proteins and transcripts in single cells | Nature biotechnology | 28854175 | Cryopreserved PBMCs from a healthy donor (AllCells, PB003F, LotA5051) were thawed at 37 C for 3 min and resuspended in 10 ml of PBS with 1% Bovine Serum Albumin (BSA) ( Save | Full Paper |
2017 | Zheng, C;Zheng, L;Yoo, JK;Guo, H;Zhang, Y;Guo, X;Kang, B;Hu, R;Huang, JY;Zhang, Q;Liu, Z;Dong, M;Hu, X;Ouyang, W;Peng, J;Zhang, Z; | Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing | Cell | 28622514 | Anti-FOXP3 antibody (IHC) Abcam Cat#ab22510 CD3 Functional Grade Monoclonal Antibody eBioscience Cat#16-0037-85 CD28 Functional Grade Monoclonal Antibody eBioscience Cat#16-0289-85 Biological Samples Human PBMC AllCells https://www.allcells.com | Full Paper |
2017 | Sinha, G; | The organoid architect | Science (New York, N.Y.) | 28839056 | After almost a decade of tedious experiments, Clevers's postdoc Nick Barker struckgold in 2007: He discovered that cells carrying a receptor named LGR5 give rise to allcells in mouse Save | Full Paper |
2017 | Stoeckius, M;Hafemeister, C;Stephenson, W;Houck-Loomis, B;Chattopadhyay, PK;Swerdlow, H;Satija, R;Smibert, P; | Simultaneous epitope and transcriptome measurement in single cells | Nature methods | 28759029 | ononuclear cells (CBMCs) were isolated from cord blood (New York Blood Center) as described25. Cells were kept on ice during and after isolation. Peripheral blood mononuclear cells were obtained from Allcells (USA). Comparing flow cytometry and CITE | Full Paper |
2017 | Seet, CS;He, C;Bethune, MT;Li, S;Chick, B;Gschweng, EH;Zhu, Y;Kim, K;Kohn, DB;Baltimore, D;Crooks, GM;Montel-Hagen, A; | Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids | Nature methods | 28369043 | placental units from deliveries at UCLA. Bone marrow (BM) was obtained from healthy adult donors (ages 18-51) through discarded material from allogeneic BM donor harvests at UCLA or purchased from AllCells Inc. (Alameda, CA). G-CSF mobilized peripher | Full Paper |
2017 | Mumbach, MR;Satpathy, AT;Boyle, EA;Dai, C;Gowen, BG;Cho, SW;Nguyen, ML;Rubin, AJ;Granja, JM;Kazane, KR;Wei, Y;Nguyen, T;Greenside, PG;Corces, MR;Tycko, J;Simeonov, DR;Suliman, N;Li, R;Xu, J;Flynn, RA;Kundaje, A;Khavari, PA;Marson, A;Corn, JE;Quertermous, T;Greenleaf, WJ;Chang, HY; | Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements | Nature genetics | 28945252 | Normal donor human peripheral blood cells were obtained fresh from AllCells. CD4 + T cells were enriched from peripheral blood using RosetteSep Human CD4 + T Cell Enrichment Save | Full Paper |
2017 | Hao, H;Hu, S;Chen, H;Bu, D;Zhu, L;Xu, C;Chu, F;Huo, X;Tang, Y;Sun, X;Ding, BS;Liu, DP;Hu, S;Wang, M; | Loss of Endothelial CXCR7 Impairs Vascular Homeostasis and Cardiac Remodeling After Myocardial Infarction: Implications for Cardiovascular Drug Discovery | Circulation | 28154007 | Human umbilical vein endothelial cells were obtained from AllCells. The CXCL12 concentration was determined with a human CXCL12 ELISA kit (R&D Systems). Calcium release was Save | Full Paper |
2017 | Gong, X;Litchfield, LM;Webster, Y;Chio, LC;Wong, SS;Stewart, TR;Dowless, M;Dempsey, J;Zeng, Y;Torres, R;Boehnke, K;Mur, C;Marugán, C;Baquero, C;Yu, C;Bray, SM;Wulur, IH;Bi, C;Chu, S;Qian, HR;Iversen, PW;Merzoug, FF;Ye, XS;Reinhard, C;De Dios, A;Du, J;Caldwell, CW;Lallena, MJ;Beckmann, RP;Buchanan, SG; | Genomic Aberrations that Activate D-type Cyclins Are Associated with Enhanced Sensitivity to the CDK4 and CDK6 Inhibitor Abemaciclib | Cancer cell | 29232554 | All cell lines were obtained from commercial vendors and were cultured in conditions recommended by vendors (Table S8; AllCells: https://www.allcells.com/; ATCC: https://www.atcc.org/; CASTCC: http://www.cellbank.org.cn; Chempartner: | Full Paper |
2017 | McKeown, MR;Corces, MR;Eaton, ML;Fiore, C;Lee, E;Lopez, JT;Chen, MW;Smith, D;Chan, SM;Koenig, JL;Austgen, K;Guenther, MG;Orlando, DA;Lovén, J;Fritz, CC;Majeti, R; | Superenhancer Analysis Defines Novel Epigenomic Subtypes of Non-APL AML, Including an RARα Dependency Targetable by SY-1425, a Potent and Selective RARα Agonist | Cancer discovery | 28729405 | Normal donor human blood cells were obtained fresh from AllCells (Alameda, CA) and FACS-enriched. Human AML samples were obtained from patients at the Stanford Medical Center with informed consent, according to Institutional Review Board (IRB)-approv | Full Paper |
2017 | Glass, JL;Hassane, D;Wouters, BJ;Kunimoto, H;Avellino, R;Garrett-Bakelman, FE;Guryanova, OA;Bowman, R;Redlich, S;Intlekofer, AM;Meydan, C;Qin, T;Fall, M;Alonso, A;Guzman, ML;Valk, PJM;Thompson, CB;Levine, R;Elemento, O;Delwel, R;Melnick, A;Figueroa, ME; | Epigenetic Identity in AML Depends on Disruption of Nonpromoter Regulatory Elements and Is Affected by Antagonistic Effects of Mutations in Epigenetic Modifiers | Cancer discovery | 28408400 | based on sample availability. Twenty-two normal CD34+ bone marrow specimens were obtained from AllCells (Alameda, CA). In addition, 6 AML samples were obtained from the Memorial Sloan Kettering Hematologic Oncology Tissue Bank for colony forming assa | Full Paper |
2017 | Pandya, H;Shen, MJ;Ichikawa, DM;Sedlock, AB;Choi, Y;Johnson, KR;Kim, G;Brown, MA;Elkahloun, AG;Maric, D;Sweeney, CL;Gossa, S;Malech, HL;McGavern, DB;Park, JK; | Differentiation of human and murine induced pluripotent stem cells to microglia-like cells | Nature neuroscience | 28253233 | RNA from human dendritic and bone marrow macrophages (AllCells) was also used. Isolated total RNA was amplified with the WT Expression Kit (Ambion) and labeled with the Save | Full Paper |
2017 | Rozo, C;Chinenov, Y;Maharaj, RK;Gupta, S;Leuenberger, L;Kirou, KA;Bykerk, VP;Goodman, SM;Salmon, JE;Pernis, AB; | Targeting the RhoA-ROCK pathway to reverse T-cell dysfunction in SLE | Annals of the rheumatic diseases | 28283529 | In separate experiments, cryopreserved human cord blood CD4 + T lymphocytes from different donors were obtained from Allcells, LLC. CD4 + T-cell purity was >95%. Cells were Save | Full Paper |
2017 | Miyawaki, K;Iwasaki, H;Jiromaru, T;Kusumoto, H;Yurino, A;Sugio, T;Uehara, Y;Odawara, J;Daitoku, S;Kunisaki, Y;Mori, Y;Arinobu, Y;Tsuzuki, H;Kikushige, Y;Iino, T;Kato, K;Takenaka, K;Miyamoto, T;Maeda, T;Akashi, K; | Identification of unipotent megakaryocyte progenitors in human hematopoiesis | Blood | 28336526 | Human adult bone marrow (BM) samples were obtained from healthy donors or purchased from AllCells. BM samples of adult hematological malignancies, diagnosed according to Save | Full Paper |
2017 | Leong, SR;Sukumaran, S;Hristopoulos, M;Totpal, K;Stainton, S;Lu, E;Wong, A;Tam, L;Newman, R;Vuillemenot, BR;Ellerman, D;Gu, C;Mathieu, M;Dennis, MS;Nguyen, A;Zheng, B;Zhang, C;Lee, G;Chu, YW;Prell, RA;Lin, K;Laing, ST;Polson, AG; | An anti-CD3/anti-CLL-1 bispecific antibody for the treatment of acute myeloid leukemia | Blood | 27908880 | (CD) CD8+ T cells were purified from peripheral blood of a healthy human donor (ALLCells, Alameda, CA) for donor 1 and a Genentech donor for donor 2 by Ficoll density gradient Save | Full Paper |
2017 | Weiss, M; | Genome Editing Strategies to Treat Beta-Hemoglobinopathies | Blood | Frozen CD34+ cells from mobilized peripheral blood of normal individuals were purchased from AllCells. Peripheral blood CD34+ cells were acquired from SCD patients undergoing therapeutic red cell exchange at the Cancer & Hematology Centers, Texas Chi | Full Paper | |
2017 | Tisdale, J;Pierciey, F;Kamble, R;Kanter, J;Krishnamurti, L;Kwiatkowski, J;Thompson, A;Shestopalov, I;Bonner, M;Joseney-Antoine, M;Asmal, M;Walters, M; | Successful Plerixafor-Mediated Mobilization, Apheresis, and Lentiviral Vector Transduction of Hematopoietic Stem Cells in Patients with Severe Sickle Cell Disease | Blood | Background Patients with severe sickle cell disease (SCD) may benefit from β-globin gene transfer into autologous hematopoietic stem cells (HSC). Successful HBB gene transfer requires vector-mediated transduction of primitive HSCs. Ste | Full Paper | |
2017 | Fujii, S;Miura, Y;Fujishiro, A;Shindo, T;Shimazu, Y; | Human Bone Marrow Mesenchymal Stromal/Stem Cell-Derived Extracellular Vesicles Ameliorate Graft-Versus-Host Disease-Related Manifestations | Blood | Human MSC were isolated from BM samples purchased from AllCells (Alameda, CA) based on our previously published method (Biochem Biophys Res Commun 2016;469:823). EV Save | Full Paper | |
2017 | Mathiyalagan, P;Liang, Y;Kim, D;Misener, S;Thorne, T;Kamide, CE;Klyachko, E;Losordo, DW;Hajjar, RJ;Sahoo, S; | Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic Hindlimb | Circulation research | 28298297 | The cells were purified from mobilized peripheral blood mononuclear cells (AllCells LLC, Emeryville, CA) with an Isolex 300i device (Baxter Healthcare); cell purity (85% to 95%) was Save | Full Paper |
2017 | Elagib, KE;Lu, CH;Mosoyan, G;Khalil, S;Zasadzińska, E;Foltz, DR;Balogh, P;Gru, AA;Fuchs, DA;Rimsza, LM;Verhoeyen, E;Sansó, M;Fisher, RP;Iancu-Rubin, C;Goldfarb, AN; | Neonatal expression of RNA-binding protein IGF2BP3 regulates the human fetal-adult megakaryocyte transition | The Journal of clinical investigation | 28481226 | The normal donor samples purchased from the Fred Hutchinson Cancer Research Center and AllCells were originally obtained with informed consent and IRB approval at both Save | Full Paper |
2017 | Chung, SS;Eng, WS;Hu, W;Khalaj, M;Garrett-Bakelman, FE;Tavakkoli, M;Levine, RL;Carroll, M;Klimek, VM;Melnick, AM;Park, CY; | CD99 is a therapeutic target on disease stem cells in myeloid malignancies | Science translational medicine | 28123069 | consent under IRB-approved protocols. Umbilical cord blood (CB) specimens were purchased from the New York Blood Center (New York, New York). Normal human BM specimens were purchased from AllCells Inc. (Emeryville, California) | Full Paper |
2017 | Zhang, T;Chi, S;Jiang, F;Zhao, Q;Xiao, B; | A protein interaction mechanism for suppressing the mechanosensitive Piezo channels | Nature communications | 29176668 | (MEM) containing 10% FBS, non-essential amino acids, 1 mM sodium pyruvate, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin. Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured using EGM-2 gro | Full Paper |
2017 | Trabanelli, S;Chevalier, MF;Martinez-Usatorre, A;Gomez-Cadena, A;Salomé, B;Lecciso, M;Salvestrini, V;Verdeil, G;Racle, J;Papayannidis, C;Morita, H;Pizzitola, I;Grandclément, C;Bohner, P;Bruni, E;Girotra, M;Pallavi, R;Falvo, P;Leibundgut, EO;Baerlocher, GM;Carlo-Stella, C;Taurino, D;Santoro, A;Spinelli, O;Rambaldi, A;Giarin, E;Basso, G;Tresoldi, C;Ciceri, F;Gfeller, D;Akdis, CA;Mazzarella, L;Minucci, S;Pelicci, PG;Marcenaro, E;McKenzie, ANJ;Vanhecke, D;Coukos, G;Mavilio, D;Curti, A;Derré, L;Jandus, C; | Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis | Nature communications | 28928446 | Human CD34 + cells were purchased from either Lonza (CH) or AllCells (USA). NSG mice were purchased from The Jackson Laboratory (stock number: 005557) and bred, treated and Save | Full Paper |
2017 | Zheng, GX;Terry, JM;Belgrader, P;Ryvkin, P;Bent, ZW;Wilson, R;Ziraldo, SB;Wheeler, TD;McDermott, GP;Zhu, J;Gregory, MT;Shuga, J;Montesclaros, L;Underwood, JG;Masquelier, DA;Nishimura, SY;Schnall-Levin, M;Wyatt, PW;Hindson, CM;Bharadwaj, R;Wong, A;Ness, KD;Beppu, LW;Deeg, HJ;McFarland, C;Loeb, KR;Valente, WJ;Ericson, NG;Stevens, EA;Radich, JP;Mikkelsen, TS;Hindson, BJ;Bielas, JH; | Massively parallel digital transcriptional profiling of single cells | Nature communications | 28091601 | s Jurkat (ATCC TIB-152), 293T (ATCC CRL-11268) and 3T3 (ATCC CRL-1658) cells were acquired from ATCC and cultured according to ATCC guidelines. Fresh PBMCs, frozen PBMCs and BMMCs were purchased from ALLCELLS. Frozen PBMCs from Donor A were made from | Full Paper |
2017 | Nakata, R;Shimada, H;Fernandez, GE;Fanter, R;Fabbri, M;Malvar, J;Zimmermann, P;DeClerck, YA; | Contribution of neuroblastoma-derived exosomes to the production of pro-tumorigenic signals by bone marrow mesenchymal stromal cells | Journal of extracellular vesicles | 28717423 | Human BM-derived MSCs were purchased from AllCells or isolated in our laboratory from the BM of patients with NB using a previously published protocol [10]. Cells were maintained Save | Full Paper |
2017 | Burton, OT;Stranks, AJ;Tamayo, JM;Koleoglou, KJ;Schwartz, LB;Oettgen, HC; | A humanized mouse model of anaphylactic peanut allergy | The Journal of allergy and clinical immunology | 27417025 | Cord blood-derived flow-sorted human CD34+ hematopoietic stem cells (HSCs) were purchased from AllCells (Alameda, Calif). Such cells are obtained under Institutional Review Board- or Human Subject Committee-approved protocols. Cells (5 × 104-105) wer | Full Paper |
2017 | Bolisetty, M;Stitzel, M;Robson, P; | CellView: Interactive exploration of high dimensional single cell RNA-seq data | bioRxiv | Single cell RNA-seq - PBMCs were purchased from AllCells, thawed quickly at 37C and into DMEM supplemented with 10% FBS. Cells were quickly spun down at 400g, for 10min. Save | Full Paper | |
2017 | Li, S;Siriwon, N;Zhang, X;Yang, S;Jin, T;He, F;Kim, YJ;Mac, J;Lu, Z;Wang, S;Han, X;Wang, P; | Enhanced Cancer Immunotherapy by Chimeric Antigen Receptor-Modified T Cells Engineered to Secrete Checkpoint Inhibitors | Clinical cancer research : an official journal of the American Association for Cancer Research | 28912137 | Chimeric antigen receptor (CAR) T-cell based therapy has shown promise as an immunotherapeutic modality for cancers. It has achieved successful responses in patients with certain Save | Full Paper |
2017 | Dai, X;Yan, J;Fu, X;Pan, Q;Sun, D;Xu, Y;Wang, J;Nie, L;Tong, L;Shen, A;Zheng, M;Huang, M;Tan, M;Liu, H;Huang, X;Ding, J;Geng, M; | Aspirin Inhibits Cancer Metastasis and Angiogenesis via Targeting Heparanase | Clinical cancer research : an official journal of the American Association for Cancer Research | 28710312 | from the Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences); primary human umbilical vascular endothelial cells (HUVECs) were purchased from AllCells. CHO- Save | Full Paper |
2017 | Das, DS;Das, A;Ray, A;Song, Y;Samur, MK;Munshi, NC;Chauhan, D;Anderson, KC; | Blockade of Deubiquitylating Enzyme USP1 Inhibits DNA Repair and Triggers Apoptosis in Multiple Myeloma Cells | Clinical cancer research : an official journal of the American Association for Cancer Research | 28270494 | Purpose: The ubiquitin proteasome pathway is a validated therapeutic target in multiple myeloma. Deubiquitylating enzyme USP1 participates in DNA damage response and cellular Save | Full Paper |
2017 | Liu, L;Lam, CK;Long, V;Widjaja, L;Yang, Y;Li, H;Jin, L;Burke, S;Gorlatov, S;Brown, J;Alderson, R;Lewis, MD;Nordstrom, JL;Koenig, S;Moore, PA;Johnson, S;Bonvini, E; | MGD011, A CD19 x CD3 Dual-Affinity Retargeting Bi-specific Molecule Incorporating Extended Circulating Half-life for the Treatment of B-Cell Malignancies | Clinical cancer research : an official journal of the American Association for Cancer Research | 27663593 | Cryopreserved purified primary CLL patient samples were from AllCells, LLC. Heparinized whole blood from cynomolgus monkeys was from Worldwide Primates, Inc. Save | Full Paper |
2017 | DeRyckere, D;Lee-Sherick, AB;Huey, MG;Hill, AA;Tyner, JW;Jacobsen, KM;Page, LS;Kirkpatrick, GG;Eryildiz, F;Montgomery, SA;Zhang, W;Wang, X;Frye, SV;Earp, HS;Graham, DK; | UNC2025, a MERTK Small-Molecule Inhibitor, Is Therapeutically Effective Alone and in Combination with Methotrexate in Leukemia Models | Clinical cancer research : an official journal of the American Association for Cancer Research | 27649555 | Deidentified cord blood and normal bone marrow samples were obtained commercially from Clinimmune Labs and ALLCELLS, respectively. Save | Full Paper |
2017 | Stoeckius, M;Hafemeister, C;Stephenson, W;Houck-Loomis, B;Chattopadhyay, P;Swerdlow, H;Satija, R;Smibert, P; | Large-scale simultaneous measurement of epitopes and transcriptomes in single cells | bioRxiv | Cord blood mononuclear cells (CBMCs) were isolated from cord blood (New York Blood Center) as described30. Cells were kept on ice during and after isolation. Peripheral blood mononuclear cells were obtained from Allcells (USA) | Full Paper | |
2017 | Cichocki, F;Valamehr, B;Bjordahl, R;Zhang, B;Rezner, B;Rogers, P;Gaidarova, S;Moreno, S;Tuininga, K;Dougherty, P;McCullar, V;Howard, P;Sarhan, D;Taras, E;Schlums, H;Abbot, S;Shoemaker, D;Bryceson, YT;Blazar, BR;Wolchko, S;Cooley, S;Miller, JS; | GSK3 Inhibition Drives Maturation of NK Cells and Enhances Their Antitumor Activity | Cancer research | 28790065 | Peripheral blood mononuclear cells (PBMCs) from healthy CMV seropositive donors were obtained from Memorial Blood Bank (Minneapolis, MN), AllCells Inc. (Alameda, CA) and Key Biologics (Memphis, TN). All samples obtained with written consent and were | Full Paper |
2017 | Jiang, H;Rivera-Molina, Y;Gomez-Manzano, C;Clise-Dwyer, K;Bover, L;Vence, LM;Yuan, Y;Lang, FF;Toniatti, C;Hossain, MB;Fueyo, J; | Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination | Cancer research | 28566332 | Oncolytic viruses selectively lyse tumor cells, disrupt immunosuppression within the tumor, and reactivate antitumor immunity, but they have yet to live up to their therapeutic potential. Save | Full Paper |
2017 | Chen, Y;Shan, Y;Lu, M;DeSouza, N;Guo, Z;Hoffman, R;Liang, A;Li, S; | Alox5 Blockade Eradicates JAK2V617F-Induced Polycythemia Vera in Mice | Cancer research | 27784744 | GE Healthcare) and low-density mononuclear cells were separated after centrifugation. The CD34+ cell population was isolated using a human CD34+ cell selection kit (StemCell Technologies) according to the manufacturer's instructions. Normal human CD3 | Full Paper |
2017 | Egidio, C;Gonzales, M;Brockman, J;Chen, S;Durruthy-Durruthy, R;Rogers, C;Ray, M; | Abstract LB-078: Improved single-cell mRNA sequencing for transcriptome and paired-chain TCR analysis of primary human CD3+ T cells | Cancer Research | The low RNA content of single human T cells has limited the success of single-cell transcriptome profiling, an approach that can generate data to reconstruct paired α,β T cell receptor (TCR) chains¹. Single-cell TCR sequencing is a powerful molecular | Full Paper | |
2017 | Chen, Q;Lawrenz, A;Wang, A;Kelly, E;Harberts, E;Ernst, R;Tong, J; | Abstract 4021: Donor-specific primary monocyte-derived dendritic cell maturation model for high-throughput screening of immunostimulatory adjuvants | Cancer Research | 1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++2University of Maryland-Baltimore, Baltimore, MD.++2University of Maryland-Baltimore, Baltimore, MD.++1AllCells, Alameda, CA | Full Paper | |
2017 | Han, X;Cinay, GE;Zhao, Y;Guo, Y;Zhang, X;Wang, P; | Adnectin-Based Design of Chimeric Antigen Receptor for T Cell Engineering | Molecular therapy : the journal of the American Society of Gene Therapy | 28784559 | Human PBMCs from healthy donors were obtained from AllCells. PBMCs were cultured in T cell medium (TCM) consisting of X-Vivo 15 (Lonza) supplemented with 5% human AB serum Save | Full Paper |
2017 | Tran, R;Myers, DR;Denning, G;Shields, JE;Lytle, AM;Alrowais, H;Qiu, Y;Sakurai, Y;Li, WC;Brand, O;Le Doux, JM;Spencer, HT;Doering, CB;Lam, WA; | Microfluidic Transduction Harnesses Mass Transport Principles to Enhance Gene Transfer Efficiency | Molecular therapy : the journal of the American Society of Gene Therapy | 28780274 | vices and wells for at least 72 hr before assessing GFP expression of transduced cells with a BD C6 Accuri flow cytometer. Primary Human T Cell Culture Frozen human pan T cells were purchased from AllCells. Cells were thawed following the AllCells t | Full Paper |
2017 | Han, X;Bryson, PD;Zhao, Y;Cinay, GE;Li, S;Guo, Y;Siriwon, N;Wang, P; | Masked Chimeric Antigen Receptor for Tumor-Specific Activation | Molecular therapy : the journal of the American Society of Gene Therapy | 28129121 | Frozen human peripheral blood mononuclear cells (PBMCs) were obtained from AllCells. PBMCs were thawed in TCM and rested overnight. Before retroviral transduction, PBMCs were Save | Full Paper |
2017 | Liu, X;Rothe, K;Yen, R;Fruhstorfer, C;Maetzig, T;Chen, M;Forrest, DL;Humphries, RK;Jiang, X; | A novel AHI-1-BCR-ABL-DNM2 complex regulates leukemic properties of primitive CML cells through enhanced cellular endocytosis and ROS-mediated autophagy | Leukemia | 28366933 | PB or BM cells were obtained from newly diagnosed patients and healthy adult donors (ALLCELLS). Informed consent was obtained in accordance with the Declaration of Helsinki, and Save | Full Paper |
2017 | Mosoyan, G;Kraus, T;Ye, F;Eng, K;Crispino, JD;Hoffman, R;Iancu-Rubin, C; | Imetelstat, a telomerase inhibitor, differentially affects normal and malignant megakaryopoiesis | Leukemia | 28270692 | Human MKs were generated using a two-step liquid culture system of either bone marrow (BM) or peripheral blood (PB) derived CD34+ cells (AllCells, LLC, Emeryville, CA, USA) as previously described.21,22 CFU-MK assays were performed by using the MegaC | Full Paper |
2017 | de Vasconcellos, JF;Tumburu, L;Byrnes, C;Lee, YT;Xu, PC;Li, M;Rabel, A;Clarke, BA;Guydosh, NR;Proia, RL;Miller, JL; | IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts | Proceedings of the National Academy of Sciences of the United States of America | 28652347 | Digestive and Kidney Diseases Institutional Intramural Review Board. CD34(+) cells from adult human healthy volunteers and human cord blood CD34(+) cells obtained from AllCells were studied ex vivo using a serum-free 21-day culture system as previous | Full Paper |
2017 | Klinker, MW;Marklein, RA;Lo Surdo, JL;Wei, CH;Bauer, SR; | Morphological features of IFN-γ-stimulated mesenchymal stromal cells predict overall immunosuppressive capacity | Proceedings of the National Academy of Sciences of the United States of America | 28283659 | PBMCs used in this study (AllCells; PB006F) were collected from healthy adult human donors. To activate T cells, 10 5 beads coated with anti-CD3 and anti-CD28 (Life Technologies; Save | Full Paper |
2017 | Frescas, D;Roux, CM;Aygun-Sunar, S;Gleiberman, AS;Krasnov, P;Kurnasov, OV;Strom, E;Virtuoso, LP;Wrobel, M;Osterman, AL;Antoch, MP;Mett, V;Chernova, OB;Gudkov, AV; | Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibody | Proceedings of the National Academy of Sciences of the United States of America | 28193858 | Human NDFs were purchased from AllCells and maintained in DMEM supplemented with 10% (vol/vol) FBS serum, 100 units/mL of penicillin, 100 µg/mL of streptomycin, and 2 mM l- Save | Full Paper |
2017 | Zhang, H;Gregorio, JD;Iwahori, T;Zhang, X;Choi, O;Tolentino, LL;Prestwood, T;Carmi, Y;Engleman, EG; | A distinct subset of plasmacytoid dendritic cells induces activation and differentiation of B and T lymphocytes | Proceedings of the National Academy of Sciences of the United States of America | 28167780 | Stanford Blood Center. Human cord blood and bone marrow samples were purchased from AllCells. Human tonsil cells were obtained from the Stanford Tissue Bank. Human subjects protocols were approved by Stanford’s Institutional Review Board (Palo Alto, | Full Paper |
2017 | Nada, MH;Wang, H;Workalemahu, G;Tanaka, Y;Morita, CT; | Enhancing adoptive cancer immunotherapy with Vγ2Vδ2 T cells through pulse zoledronate stimulation | Journal for immunotherapy of cancer | 28239463 | random healthy adult donors were obtained by density centrifugation over Ficoll-Hypaque (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) of either random donor leukopaks (12 donors) (obtained from AllCells, LLC., Alameda, CA or the Blood Donor Cent | Full Paper |
2017 | Kuramitsu, M;Sekizuka, T;Yamochi, T;Firouzi, S;Sato, T;Umeki, K;Sasaki, D;Hasegawa, H;Kubota, R;Sobata, R;Matsumoto, C;Kaneko, N;Momose, H;Araki, K;Saito, M;Nosaka, K;Utsunomiya, A;Koh, KR;Ogata, M;Uchimaru, K;Iwanaga, M;Sagara, Y;Yamano, Y;Okayama, A;Miura, K;Satake, M;Saito, S;Itabashi, K;Yamaguchi, K;Kuroda, M;Watanabe, T;Okuma, K;Hamaguchi, I; | Proviral Features of Human T Cell Leukemia Virus Type 1 in Carriers with Indeterminate Western Blot Analysis Results | Journal of clinical microbiology | 28701419 | AllCells Inc. (Alameda, CA, USA). Cryopreserved PBMCs were resuspended in RPMI 1640 supplemented with 10% FBS at 37C in accordance with the protocol provided by AllCells Inc. Save | Full Paper |
2017 | Gonçalves Silva, I;Yasinska, IM;Sakhnevych, SS;Fiedler, W;Wellbrock, J;Bardelli, M;Varani, L;Hussain, R;Siligardi, G;Ceccone, G;Berger, SM;Ushkaryov, YA;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV; | The Tim-3-galectin-9 Secretory Pathway is Involved in the Immune Escape of Human Acute Myeloid Leukemia Cells | EBioMedicine | 28750861 | Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordance with the Save;ultured in Stem-Pro-34 serum-free media in the presence of 100 ng/ml SCF (Kirshenba | Full Paper |
2017 | Alvey, CM;Spinler, KR;Irianto, J;Pfeifer, CR;Hayes, B;Xia, Y;Cho, S;Dingal, PCPD;Hsu, J;Smith, L;Tewari, M;Discher, DE; | SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid Tumors | Current biology : CB | 28669759 | Subject 1: Female age 30, subject 2: Female age 31, subject 3: Male age 31, subject 4: Male age 22 with additional details provided by AllCells per (Figure 7A). Marrow was pooled and Save | Full Paper |
2017 | Fujishiro, A;Miura, Y;Iwasa, M;Fujii, S;Sugino, N;Andoh, A;Hirai, H;Maekawa, T;Ichinohe, T; | Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells | Inflammation and regeneration | 29259718 | e 5E10), and anti-CD105 (clone SN6) antibodies were from eBioscience (San Diego, CA). Isolation, expansion, and γ-irradiation of BM-MSCs BM samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA). BM-MSCs were isolated ba | Full Paper |
2017 | Yang, C;Wang, X;Ma, B;Zhu, H;Huan, Z;Ma, N;Wu, C;Chang, J; | 3D-Printed Bioactive Ca3SiO5 Bone Cement Scaffolds with Nano Surface Structure for Bone Regeneration | ACS applied materials & interfaces | 28117976 | Rat bone-marrow stem cells (rBMSCs) (obtained from Allcells, Silicon Valley, CA) were seeded with an initial density of 1× 104 on each scaffold in 48 well tissue culture plates containing 500 μL of Dulbecco’s modified Eagle’s medium (DMEM, Gibco) sup | Full Paper |
2017 | Gautron, AS;Juillerat, A;Guyot, V;Filhol, JM;Dessez, E;Duclert, A;Duchateau, P;Poirot, L; | Fine and Predictable Tuning of TALEN Gene Editing Targeting for Improved T Cell Adoptive Immunotherapy | Molecular therapy. Nucleic acids | 29246309 | eBioscience. All the cytometry analyses were performed on a FACSCanto II (BD Biosciences). Cell Culture Frozen peripheral blood mononuclear cells (PBMC) were obtained from healthy volunteer donors (Allcells). T lymphocytes were activated directly fr | Full Paper |
2017 | Shim, H; | Hypoxia-Inducible Factor-1 | Encyclopedia of Cancer | In brief, CD34-depleted human cord blood mononuclear cells (#CB117; AllCells) were infected in vitro with the M81 strain of EBV (2,000 GRU) by incubation at 37C for 1.5 h after which Save | Full Paper | |
2017 | Zhang, W;Zhou, L;Shi, J;Deng, H; | Fabrication of novel visible-light-driven AgI/g-C(3)N(4) composites with enhanced visible-light photocatalytic activity for diclofenac degradation | Journal of colloid and interface science | 28222304 | Diclofenac sodium (DCF) was purchased from the Sigma Chemical Reagent Co., US Human umbilical vein endothelial cells (HUVECs) were obtained from Allcells, Shanghai, China. Save | Full Paper |
2017 | Shi, J;Deng, H;Pan, H;Xu, Y;Zhang, M; | Epigallocatechin-3-gallate attenuates microcystin-LR induced oxidative stress and inflammation in human umbilical vein endothelial cells | Chemosphere | 27776235 | Primary HUVECs were obtained from Allcells (Shanghai, China) and cells in passages 3-5 were used in this study. Cells were grown in an RPMI 1640 medium containing 20% FBS ( Save | Full Paper |
2017 | Ali, AJ;Abuelela, AF;Merzaban, JS; | An Analysis of Trafficking Receptors Shows that CD44 and P-Selectin Glycoprotein Ligand-1 Collectively Control the Migration of Activated Human T-Cells | Frontiers in immunology | 28515724 | Frozen normal peripheral blood mononuclear cells isolated from subjects with and without psoriasis were ordered from AllCells (CA, USA). Naive Pan T-cell Isolation Kit and Pan T-cell Save | Full Paper |
2017 | Guo, H;Zhu, Q;Yu, X;Merugu, SB;Mangukiya, HB;Smith, N;Li, Z;Zhang, B;Negi, H;Rong, R;Cheng, K;Wu, Z;Li, D; | Tumor-secreted anterior gradient-2 binds to VEGF and FGF2 and enhances their activities by promoting their homodimerization | Oncogene | 28481872 | HUVECs were purchased from AllCells Company and maintained in HUVEC complete medium (AllCells, Shanghai, China). Cells were passaged for fewer than six passages. THP-1 Save | Full Paper |
2017 | Winter, MB;La Greca, F;Arastu-Kapur, S;Caiazza, F;Cimermancic, P;Buchholz, TJ;Anderl, JL;Ravalin, M;Bohn, MF;Sali, A;O'Donoghue, AJ;Craik, CS; | Immunoproteasome functions explained by divergence in cleavage specificity and regulation | eLife | 29182146 | on the list of commonly misidentified cell lines from the International Cell Line Authentication Committee. Primary human peripheral blood mononuclear cells (PBMCs) (cat. PB005F) were purchased from AllCells (Alameda, CA). The SUP-B15 cell line was p | Full Paper |
2017 | Bak, RO;Dever, DP;Reinisch, A;Cruz Hernandez, D;Majeti, R;Porteus, MH; | Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR/Cas9 and AAV6 | eLife | 28956530 | Frozen CD34+ HSPCs derived from mobilized peripheral blood or cord blood were purchased from AllCells (Alameda, CA, USA) and thawed according to manufacturer's instructions. Save | Full Paper |
2017 | Kieffer, C;Ladinsky, MS;Ninh, A;Galimidi, RP;Bjorkman, PJ; | Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomography | eLife | 28198699 | s used at a dilution of 1:500 and 10 nm gold-conjugated goat anti-rabbit IgG (Ted Pella) was used at a dilution of 1:20. Flow cytometry of primary and reconstituted human PBMCs Cultured human PBMCs (AllCells, inc.) were analyzed by flow cytometry (M | Full Paper |
2017 | Li, Y;Li, X;Zhao, R;Wang, C;Qiu, F;Sun, B;Ji, H;Qiu, J;Wang, C; | Enhanced adhesion and proliferation of human umbilical vein endothelial cells on conductive PANI-PCL fiber scaffold by electrical stimulation | Materials science & engineering. C, Materials for biological applications | 28024565 | Human umbilical vein endothelial cells (HUVECs, AllCells Biological Technology Co., Ltd. Shanghai) were cultured in complete Medium. During the cell culture, the culture medium was refreshed every 2- 3 days. HUVECs at passage 2- 3 were used in this w | Full Paper |
2017 | Xu, JG;Zhu, SY;Heng, BC;Dissanayaka, WL;Zhang, CF; | TGF-β1-induced differentiation of SHED into functional smooth muscle cells | Stem cell research & therapy | 28114966 | f this study was to differentiate SHED into SMCs by TGF-β1 alone or in combination with BMP4, and to investigate the functionality of the derived SMCs. Methods Cell culture SHED were purchased from ALLCELLS (Alameda, CA, USA), and were evaluated for | Full Paper |
2017 | Schlatzer, D;Haqqani, AA;Li, X;Dobrowolski, C;Chance, MR;Tilton, JC; | A Targeted Mass Spectrometry Assay for Detection of HIV Gag Protein Following Induction of Latent Viral Reservoirs | Analytical chemistry | 28467046 | Normal donor samples were obtained from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated using RosetteSep CD4+ T cell enrichment kit Save | Full Paper |
2017 | Tan, S;Shan, Y;Wang, Y;Lin, Y;Liao, S;Deng, Z;Zhou, L;Cai, W;Zeng, Q;Zhang, L;Zhang, B;Men, X;Li, H;Hu, X;Wu, C;Peng, L;Lu, Z; | Exacerbation of oxygen-glucose deprivation-induced blood-brain barrier disruption: potential pathogenic role of interleukin-9 in ischemic stroke | Clinical science (London, England : 1979) | 28550144 | The bEnd.3 cells were purchased from AllCells and grown to subconfluence before the exposure to oxygen-glucose deprivation (OGD: 94.95% N2, 0.05% O2, 5% CO2) or normoxic conditions (75% N2, 20% O2, 5% CO2) for 4 or 24 h. Cells were divided into the f | Full Paper |
2017 | Gregg, KA;Harberts, E;Gardner, FM;Pelletier, MR;Cayatte, C;Yu, L;McCarthy, MP;Marshall, JD;Ernst, RK; | Rationally Designed TLR4 Ligands for Vaccine Adjuvant Discovery | mBio | 28487429 | The dendritic cell (DC) maturation experiments were contracted to and run by AllCells (Alameda, CA) using standard company protocols. Briefly, human PBMCs from 3 separate donors Save | Full Paper |
2017 | Wang, K;Jiang, Z;Webster, KA;Chen, J;Hu, H;Zhou, Y;Zhao, J;Wang, L;Wang, Y;Zhong, Z;Ni, C;Li, Q;Xiang, C;Zhang, L;Wu, R;Zhu, W;Yu, H;Hu, X;Wang, J; | Enhanced Cardioprotection by Human Endometrium Mesenchymal Stem Cells Driven by Exosomal MicroRNA-21 | Stem cells translational medicine | 28170197 | ngzhou, China, http://www.sebio.net.cn). Human AdMSCs were purchased from Cyagen Bioscience (Guangzhou, China, http://www.cyagen.com). Human umbilical vein endothelial cells (HUVECs) were provided by AllCells (Shanghai, China, http://www.allcells.com | Full Paper |
2017 | Sebastian, NT;Zaikos, TD;Terry, V;Taschuk, F;McNamara, LA;Onafuwa-Nuga, A;Yucha, R;Signer, RAJ;Riddell, J;Bixby, D;Markowitz, N;Morrison, SJ;Collins, KL; | CD4 is expressed on a heterogeneous subset of hematopoietic progenitors, which persistently harbor CXCR4 and CCR5-tropic HIV proviral genomes in vivo | PLoS pathogens | 28732051 | from each donor. All collected samples were coded. Whole umbilical cord blood (CB) from uninfected donors was obtained from the New York Blood Center and whole bone marrow was obtained commercially (AllCells Ltd.). All collected samples were anonymi | Full Paper |
2017 | Kraus, RJ;Yu, X;Cordes, BA;Sathiamoorthi, S;Iempridee, T;Nawandar, DM;Ma, S;Romero-Masters, JC;McChesney, KG;Lin, Z;Makielski, KR;Lee, DL;Lambert, PF;Johannsen, EC;Kenney, SC;Mertz, JE; | Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter | PLoS pathogens | 28617871 | In brief, CD34-depleted human cord blood mononuclear cells (#CB117; AllCells) were infected in vitro with the M81 strain of EBV (2,000 GRU) by incubation at 37C for 1.5 h after which Save | Full Paper |
2017 | Ganaie, SS;Zou, W;Xu, P;Deng, X;Kleiboeker, S;Qiu, J; | Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex | PLoS pathogens | 28459842 | ti-viral prophylaxis of transplant recipients. Materials and methods Ethics statement We purchased CD34+ hematopoietic stem cells, which were isolated from bone marrow of a healthy human donor, from AllCells LLC (Alameda, CA) without any identificat | Full Paper |
2017 | Nie, Y;Ran, Y;Zhang, HY;Huang, ZF;Pan, ZY;Wang, SY;Wang, YY; | GPATCH3 negatively regulates RLR-mediated innate antiviral responses by disrupting the assembly of VISA signalosome | PLoS pathogens | 28414768 | University, Cambridge, MA). HEK293T (Transformed Human Embryonic Kidney 293 cell line, ATCC) cells, A549 (Human Lung Adenocarcinoma cell line, ATCC) cells, PBMCs (Peripheral Blood Mononuclear Cells, Allcells), Human recombinant TNFα (R&D Systems), po | Full Paper |
2017 | Xu, P;Zhou, Z;Xiong, M;Zou, W;Deng, X;Ganaie, SS;Kleiboeker, S;Peng, J;Liu, K;Wang, S;Ye, SQ;Qiu, J; | Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway | PLoS pathogens | 28264028 | We purchased the cells at AllCells LLC. (www.allcells.com, Alameda, CA), which were all anonymized, and, therefore, an IRB review was waived. Save | Full Paper |
2017 | Morin, J;Dubey, N;Decroix, F;Luong-Van, E;Castro Neto, A;Rosa, V; | Graphene transfer to 3-dimensional surfaces: a vacuum-assisted dry transfer method | 2D Materials | Dental pulp stem cells (DPSC) (AllCells, USA) of passage 4 were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen, USA), supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin/streptomycin (Invitrogen). Disinfection of the i | Full Paper | |
2017 | Ma, SD;Tsai, MH;Romero-Masters, JC;Ranheim, EA;Huebner, SM;Bristol, JA;Delecluse, HJ;Kenney, SC; | Latent Membrane Protein 1 (LMP1) and LMP2A Collaborate To Promote Epstein-Barr Virus-Induced B Cell Lymphomas in a Cord Blood-Humanized Mouse Model but Are Not Essential | Journal of virology | 28077657 | Commercially purchased CD34depleted human cord blood mononuclear cells (CB117; AllCells, LLC) were mock infected or infected with WT or mutant viruses in vitro for 1.5 h, and 12 Save | Full Paper |
2017 | Adler, AS;Mizrahi, RA;Spindler, MJ;Adams, MS;Asensio, MA;Edgar, RC;Leong, J;Leong, R;Roalfe, L;White, R;Goldblatt, D;Johnson, DS; | Rare, high-affinity anti-pathogen antibodies from human repertoires, discovered using microfluidics and molecular genomics | mAbs | 28846502 | For influenza A vaccinated libraries, we contracted AllCells to vaccinate three donors (ages For the pneumococcus vaccinated libraries, we contracted AllCells to vaccinate five donors ( Save | Full Paper |
2017 | Urbinati, F;Wherley, J;Geiger, S;Fernandez, BC;Kaufman, ML;Cooper, A;Romero, Z;Marchioni, F;Reeves, L;Read, E;Nowicki, B;Grassman, E;Viswanathan, S;Wang, X;Hollis, RP;Kohn, DB; | Preclinical studies for a phase 1 clinical trial of autologous hematopoietic stem cell gene therapy for sickle cell disease | Cytotherapy | 28733131 | Control healthy donor BM samples were purchased from commercial sources (AllCells or Hemacare) obtaining aspirates from volunteer donors. The ages of the five healthy donors Save | Full Paper |
2017 | Pachler, K;Lener, T;Streif, D;Dunai, ZA;Desgeorges, A;Feichtner, M;Öller, M;Schallmoser, K;Rohde, E;Gimona, M; | A Good Manufacturing Practice-grade standard protocol for exclusively human mesenchymal stromal cell-derived extracellular vesicles | Cytotherapy | 28188071 | The second BM aspirate was purchased from AllCells. Both donors had signed an informed consent. BM-MSCs were isolated from BM as described [30] and tested negative for Save | Full Paper |
2017 | Khairani, M;Nur, N;Basma, M;Widya, L;Solachuddin Jauhari, I;Deny, D;Azlina, A;Thirumulu, K; | Effects of flaxseed extract on the viability of stem cells from human extracted deciduous teeth (SHED) | Frontiers in Bioengineering and Biotechnology | SHED (AllCells, USA) was previously cultured and maintained in alpha-MEM (GIBCO, USA) containing 10% FBS (GIBCO, USA) and 0.5% penicillin/streptomycin (GIBCO, USA) until Save | Full Paper | |
2017 | Lifshitz, V;Priceman, SJ;Li, W;Cherryholmes, G;Lee, H;Makovski-Silverstein, A;Borriello, L;DeClerck, YA;Yu, H; | Sphingosine-1-Phosphate Receptor-1 Promotes Environment-Mediated and Acquired Chemoresistance | Molecular cancer therapeutics | 28716816 | Human BM-MSC were either purchased from ALLCells LLC or obtained from fresh ALLCells, which were maintained in basal medium with growth supplements obtained from ALLCells. Save | Full Paper |
2017 | Zhao, H;Gulesserian, S;Ganesan, SK;Ou, J;Morrison, K;Zeng, Z;Robles, V;Snyder, J;Do, L;Aviña, H;Karki, S;Stover, DR;Doñate, F; | Inhibition of Megakaryocyte Differentiation by Antibody-Drug Conjugates (ADCs) is Mediated by Macropinocytosis: Implications for ADC-induced Thrombocytopenia | Molecular cancer therapeutics | 28655784 | ABM026F) isolated from bone marrow were purchased from ALLCELLS. All HSC growth media (StemSpan SFEM II, catalog no. 09600), supplements for expansion (CC100, catalog no. Save | Full Paper |
2017 | Ao, JY;Zhu, XD;Chai, ZT;Cai, H;Zhang, YY;Zhang, KZ;Kong, LQ;Zhang, N;Ye, BG;Ma, DN;Sun, HC; | Colony-Stimulating Factor 1 Receptor Blockade Inhibits Tumor Growth by Altering the Polarization of Tumor-Associated Macrophages in Hepatocellular Carcinoma | Molecular cancer therapeutics | 28572167 | A human umbilical vein endothelial cell (HUVEC) line was purchased from Allcells and was grown in Allcells completed medium supplemented with 10% FBS. All cell lines obtained Save | Full Paper |
2017 | Zhao, H;Gulesserian, S;Malinao, MC;Ganesan, SK;Song, J;Chang, MS;Williams, MM;Zeng, Z;Mattie, M;Mendelsohn, BA;Stover, DR;Doñate, F; | A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own Demise | Molecular cancer therapeutics | 28522588 | Human hematopoietic stem cells (CD133+, catalog no. ABM026F) isolated from bone marrow and human mature neutrophils (catalog no. PB016) were purchased from ALLCELLS. HSC growth media (StemSpan SFEM II, catalog no. 09600), supplements for expansion (C | Full Paper |
2017 | Tao, B;Wang, R;Sun, C;Zhu, Y; | 3-Mercaptopyruvate Sulfurtransferase, Not Cystathionine β-Synthase Nor Cystathionine γ-Lyase, Mediates Hypoxia-Induced Migration of Vascular Endothelial Cells | Frontiers in pharmacology | 28979207 | et al., 2007). Materials and methods Cell culture Primary human umbilical vein endothelial cells (HUVECs) and culture medium (supplemented with or without 10% fetal bovine serum) were purchased from Allcells (Shanghai, China). Cells were cultured in | Full Paper |
2017 | Hall, BM;Balan, V;Gleiberman, AS;Strom, E;Krasnov, P;Virtuoso, LP;Rydkina, E;Vujcic, S;Balan, K;Gitlin, II;Leonova, KI;Consiglio, CR;Gollnick, SO;Chernova, OB;Gudkov, AV; | p16(Ink4a) and senescence-associated β-galactosidase can be induced in macrophages as part of a reversible response to physiological stimuli | Aging | 28768895 | enmark) where live and dead counts of nucleated cells were quantitated on a NucleoCounter NC-200 (ChemoMetec) via staining with acridine orange and DAPI. Primary human neonatal dermal fibroblasts (AllCells, LLC; Alameda, CA), pooled equally from th | Full Paper |
2017 | Bam, RA;Hansen, D;Irrinki, A;Mulato, A;Jones, GS;Hesselgesser, J;Frey, CR;Cihlar, T;Yant, SR; | TLR7 Agonist GS-9620 Is a Potent Inhibitor of Acute HIV-1 Infection in Human Peripheral Blood Mononuclear Cells | Antimicrobial agents and chemotherapy | 27799218 | e anti-human CD8 antibodies, used for flow cytometry experiments, were purchased from BD Biosciences (San Jose, CA, USA) and stored at 4°C in the dark. Primary cells. Leucopacks were purchased from AllCells (Alameda, CA, USA) and were obtained from | Full Paper |
2017 | Kota, DJ;Prabhakara, KS;Toledano-Furman, N;Bhattarai, D;Chen, Q;DiCarlo, B;Smith, P;Triolo, F;Wenzel, PL;Cox, CS;Olson, SD; | Prostaglandin E2 Indicates Therapeutic Efficacy of Mesenchymal Stem Cells in Experimental Traumatic Brain Injury | Stem cells (Dayton, Ohio) | 28233425 | Donors 3305, 3388 P0 MSC were isolated from commercially available fresh human bone marrow (hBM) aspirates (AllCells, Emeryville, CA, https://www.allcells.com/); MSC from donor TBM-1 were obtained from hBM aspirates under the approved IBC protocol # | Full Paper |
2017 | Diaz, MF;Vaidya, AB;Evans, SM;Lee, HJ;Aertker, BM;Alexander, AJ;Price, KM;Ozuna, JA;Liao, GP;Aroom, KR;Xue, H;Gu, L;Omichi, R;Bedi, S;Olson, SD;Cox, CS;Wenzel, PL; | Biomechanical Forces Promote Immune Regulatory Function of Bone Marrow Mesenchymal Stromal Cells | Stem cells (Dayton, Ohio) | 28181347 | Bone marrow MSCs were derived from whole bone marrow from independent human donors (commercially available by AllCells) (Supporting Information Figure S1A). Briefly, mononuclear cells were enriched in the buffy layer of whole bone marrow by phase sep | Full Paper |
2017 | Garbuzova-Davis, S;Haller, E;Lin, R;Borlongan, CV; | Intravenously Transplanted Human Bone Marrow Endothelial Progenitor Cells Engraft Within Brain Capillaries, Preserve Mitochondrial Morphology, and Display Pinocytotic Activity Toward Blood-Brain Barrier Repair in Ischemic Stroke Rats | Stem cells (Dayton, Ohio) | 28142208 | The isolation of human bone marrow endothelial progenitor cells (hBMEPCs) was performed accordingly to the protocol reported previously [52-54] with modifications. Briefly, for each independent experiment, human bone marrows (AllCells, Almeda, CA, ht | Full Paper |
2017 | Zhang, M;Pan, H;Xu, Y;Wang, X;Qiu, Z;Jiang, L; | Allicin Decreases Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Umbilical Vein Endothelial Cells through Suppression of Mitochondrial Dysfunction and Activation of Nrf2 | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 28456799 | Commercial HUVECs were purchased from Allcells (Shanghai Biological Technology Co., Ltd., China). The cells were seeded in RPMI 1640 medium with 10 % fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 60?µg/mL endothelial cell growth suppleme | Full Paper |
2017 | Deng, H;Song, Z;Xu, H;Deng, X;Zhang, Q;Chen, H;Wang, Y;Qin, Y;Li, Y; | MicroRNA-1185 Promotes Arterial Stiffness though Modulating VCAM-1 and E-Selectin Expression | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 28441665 | The pHUVECs were obtained from AllCells (Shanghai, China) and cultured in complete medium (AllCells, Shanghai, China). The HUVSMCs were purchased from ScienCell (Carlsbad, Save | Full Paper |
2017 | Deng, H;Chu, X;Song, Z;Deng, X;Xu, H;Ye, Y;Li, S;Zhang, Q;Sun, C;Li, Y; | MicroRNA-1185 Induces Endothelial Cell Apoptosis by Targeting UVRAG and KRIT1 | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 28441650 | pHUVEC were purchased from Allcells (Shanghai, China) and maintained in complete medium (Allcells). The human endothelial cells line EA.hy926 cells (ATCC, Manassas, VA, USA) Save | Full Paper |
2017 | Shay, AE;Diwakar, BT;Guan, BJ;Narayan, V;Urban, JF;Prabhu, KS; | IL-4 up-regulates cyclooxygenase-1 expression in macrophages | The Journal of biological chemistry | 28684424 | Frozen human PBMC were purchased from AllCells (Alameda, CA) from donors. Upon arrival, cells were thawed in a 37 °C water and enumerated with trypan blue staining using a hemocytometer to determine cell viability. Cells were transferred to a 50-ml t | Full Paper |
2017 | Li, X;Anderson, M;Collin, D;Muegge, I;Wan, J;Brennan, D;Kugler, S;Terenzio, D;Kennedy, C;Lin, S;Labadia, ME;Cook, B;Hughes, R;Farrow, NA; | Structural studies unravel the active conformation of apo RORγt nuclear receptor and a common inverse agonism of two diverse classes of RORγt inhibitors | The Journal of biological chemistry | 28546429 | Frozen CD4+ T cells (AllCells) were thawed and resuspended in X-VIVO media (Lonza) at a cell density of 1 × 106 cells/ml. Skewing cytokines were added to media at the final concentrations of 30 ng/ml IL-23, 10 ng/ml IL-1β, 10 ng/ml IL-6, 2 ng/ml IL-2 | Full Paper |
2017 | Li, J;Bhattacharya, S;Zhou, J;Phadnis-Moghe, AS;Crawford, RB;Kaminski, NE; | Aryl Hydrocarbon Receptor Activation Suppresses EBF1 and PAX5 and Impairs Human B Lymphopoiesis | Journal of immunology (Baltimore, Md. : 1950) | 28978690 | Fresh human CD34 + HSPCs isolated from cord blood from mixed donors were purchased from AllCells (Emeryville, CA). The in vitro feeder-free HSPC culture was modified based on a Save | Full Paper |
2017 | Eskandarpour, M;Alexander, R;Adamson, P;Calder, VL; | Pharmacological Inhibition of Bromodomain Proteins Suppresses Retinal Inflammatory Disease and Downregulates Retinal Th17 Cells | Journal of immunology (Baltimore, Md. : 1950) | 28039300 | Enriched human PBMC-derived CD4+ T cells were obtained (AllCells; Caltag Medsystems, Buckingham, U.K.) and cultured in X-VIVO 20 and polarized to a Th17 phenotype by addition of recombinant human (rh)IL-6 (30 ng/ml), rhIL-23 (20 ng/ml), rhIL-1b (10 n | Full Paper |
2017 | Frescas, D;Hall, BM;Strom, E;Virtuoso, LP;Gupta, M;Gleiberman, AS;Rydkina, E;Balan, V;Vujcic, S;Chernova, OB;Gudkov, AV; | Murine mesenchymal cells that express elevated levels of the CDK inhibitor p16(Ink4a) in vivo are not necessarily senescent | Cell cycle (Georgetown, Tex.) | 28650766 | Human NDFs were purchased from AllCells and maintained in DMEM supplemented with 10% FBS serum, 100 units/mL of penicillin, 100 µg/mL of streptomycin, and 2 mM L-Glutamine Save | Full Paper |
2017 | Li, S;Ning, H;Ye, Y;Wei, W;Guo, R;Song, Q;Liu, L;Liu, Y;Na, L;Niu, Y;Chu, X;Feng, R;Moustaid-Moussa, N;Li, Y;Sun, C; | Increasing extracellular Ca2+ sensitizes TNF-alpha-induced vascular cell adhesion molecule-1 (VCAM-1) via a TRPC1/ERK1/2/NFκB-dependent pathway in human vascular endothelial cells | Biochimica et biophysica acta. Molecular cell research | 28583863 | Primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Cells were used between passages 3 and 6. Human umbilical vein cell line (EA.hy926) and human leukemic monocyte lymphoma cell line (U937) were obt | Full Paper |
2017 | Wang, H;Gonzalez-Garcia, I;Traba, J;Jain, S;Conteh, S;Shin, DM;Qi, C;Gao, Y;Sun, J;Kang, S;Abbasi, S;Naghashfar, Z;Yoon, J;DuBois, W;Kovalchuk, AL;Sack, MN;Duffy, P;Morse, HC; | ATP-degrading ENPP1 is required for survival (or persistence) of long-lived plasma cells | Scientific reports | 29259245 | The efficiency of reconstitution was verified by FACS analyses of blood for frequencies of B and T cells shortly before mice were immunized 8 wks later. Human bone marrow cells were purchased from Allcells (Alameda, CA) and processed for antibody st | Full Paper |
2017 | Katano, I;Nishime, C;Ito, R;Kamisako, T;Mizusawa, T;Ka, Y;Ogura, T;Suemizu, H;Kawakami, Y;Ito, M;Takahashi, T; | Long-term maintenance of peripheral blood derived human NK cells in a novel human IL-15- transgenic NOG mouse | Scientific reports | 29222435 | iminated twice using a MACS LD column (Miltenyi Biotec). The purity of the isolated human NK cells was usually greater than 95%. We also used human Caucasian PB-derived CD56+ NK cells, purchased from AllCells (Berkeley, CA, USA), in some experiments. | Full Paper |
2017 | Zhang, M;Qiu, L;Zhang, Y;Xu, D;Zheng, JC;Jiang, L; | CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells | Scientific reports | 28811579 | Human umbilical cord vein endothelial cells (HUVECs) (AllCells, Shanghai, China) were cultured for up to 5-7 passages in complete endothelial cell growth medium (H004, AllCells, Save | Full Paper |
2017 | Ma, R;Xie, R;Yu, C;Si, Y;Wu, X;Zhao, L;Yao, Z;Fang, S;Chen, H;Novakovic, V;Gao, C;Kou, J;Bi, Y;Thatte, HS;Yu, B;Yang, S;Zhou, J;Shi, J; | Phosphatidylserine-mediated platelet clearance by endothelium decreases platelet aggregates and procoagulant activity in sepsis | Scientific reports | 28694452 | a50,51. Study was performed with the approval of the Ethics Committee of Harbin Medical University in accordance with the Declaration of Helsinki. Reagents Endothelial cell medium was purchased from AllCells (Emeryville, CA, USA). Bovine serum album | Full Paper |
2017 | Garbuzova-Davis, S;Kurien, C;Thomson, A;Falco, D;Ahmad, S;Staffetti, J;Steiner, G;Abraham, S;James, G;Mahendrasah, A;Sanberg, PR;Borlongan, CV; | Endothelial and Astrocytic Support by Human Bone Marrow Stem Cell Grafts into Symptomatic ALS Mice towards Blood-Spinal Cord Barrier Repair | Scientific reports | 28408761 | t 14 weeks of age and weekly thereafter, mice again underwent behavioral testing until 17 weeks of age. Cell preparation and transplant procedure Cryopreserved human bone marrow CD34+ cells (hBM34+, AllCells, Alameda, CA, USA) were thawed rapidly at | Full Paper |
2017 | Li, XH;Xue, WL;Wang, MJ;Zhou, Y;Zhang, CC;Sun, C;Zhu, L;Liang, K;Chen, Y;Tao, BB;Tan, B;Yu, B;Zhu, YC; | H2S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression | Scientific reports | 28322298 | Primary human umbilical vein endothelial cells were purchased from Allcells (Shanghai, China) and cultured in endothelial complete medium (ECM; Allcells, China) as described Save | Full Paper |
2017 | Fang, HY;Zeng, HW;Lin, LM;Chen, X;Shen, XN;Fu, P;Lv, C;Liu, Q;Liu, RH;Zhang, WD;Zhao, J; | A network-based method for mechanistic investigation of Shexiang Baoxin Pill's treatment of cardiovascular diseases | Scientific reports | 28272527 | HUVECs were seeded into 60 mm cell culture plates with complete endothelial growth medium (AllCells). After reaching 80% confluence, cells were exposed to SBP extract and SBPpc Save | Full Paper |
2017 | Manser, M;Sater, MR;Schmid, CD;Noreen, F;Murbach, M;Kuster, N;Schuermann, D;Schär, P; | ELF-MF exposure affects the robustness of epigenetic programming during granulopoiesis | Scientific reports | 28266526 | CD34-positive cells isolated from human cord blood of mixed donors were obtained from AllCells (Alabama, United States) and propagated in Stemline II medium expansion medium ( Save | Full Paper |
2017 | Wei, G;Jie, Y;Haibo, L;Chaoneng, W;Dong, H;Jianbing, Z;Junjie, G;Leilei, M;Hongtao, S;Yunzeng, Z;Junbo, G; | Dendritic cells derived exosomes migration to spleen and induction of inflammation are regulated by CCR7 | Scientific reports | 28223684 | ons (6 μm) were prepared using a Lab-Tek tissue processor (Leica). The samples were stained with CD11c/CD4 (Abcam) and DAPI and observed with confocal microscopy. CD4+ T cells were purchased from Allcells (Shanghai, China). To determine whether T ce | Full Paper |
2017 | Rodriguez, C;Kessler, F;Dubey, N;Rosa, V;Fechine, G; | CVD graphene transfer procedure to the surface of stainless steel for stem cell proliferation | Surface and Coatings Technology | Human dental pulp stem cells (DPSC) from a single donor (DPF003, Allcells, USA) were cultured in Dulbecco's modified Eagle's medium (Invitrogen, USA), supplemented with 10% Save | Full Paper | |
2017 | Lee, HJ;Diaz, MF;Ewere, A;Olson, SD;Cox, CS;Wenzel, PL; | Focal adhesion kinase signaling regulates anti-inflammatory function of bone marrow mesenchymal stromal cells induced by biomechanical force | Cellular signalling | 28647573 | Bone marrow MSCs were derived from whole bone marrow from independent human donor (AllCells). Cells were isolated and maintained as described previously [26]. Briefly, enriched Save | Full Paper |
2017 | Killilea, DW;Rohner, F;Ghosh, S;Otoo, GE;Smith, L;Siekmann, JH;King, JC; | Identification of a Hemolysis Threshold That Increases Plasma and Serum Zinc Concentration | The Journal of nutrition | 28490675 | Blood samples from 14 healthy participants (Supplemental Table 1) were purchased from a commercial laboratory (AllCells LLC). The laboratory prescreened donors for lack of Save | Full Paper |
2017 | Bellayr, IH;Kumar, A;Puri, RK; | MicroRNA expression in bone marrow-derived human multipotent Stromal cells | BMC genomics | 28800721 | A total of 8 human bone marrow-derived MSCs from 8 donors were purchased from ALLCELLS and Lonza at passage 1 (Additional file 1: Table S1). By flow cytometry, all MSCs Save | Full Paper |
2017 | Huang, J;Khan, A;Au, BC;Barber, DL;López-Vásquez, L;Prokopishyn, NL;Boutin, M;Rothe, M;Rip, JW;Abaoui, M;Nagree, MS;Dworski, S;Schambach, A;Keating, A;West, ML;Klassen, J;Turner, PV;Sirrs, S;Rupar, CA;Auray-Blais, C;Foley, R;Medin, JA; | Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34+ Cells for Correction of Fabry Disease | Molecular therapy. Methods & clinical development | 28603745 | lutamine (PSQ) (2 mM glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin; Gibco by Life Technologies). Mobilized peripheral blood normal CD34+ cells from healthy donors were purchased from AllCells. Human primary CD34+ cells were cultured in | Full Paper |
2017 | Lawson, T;Kehoe, D;Schnitzler, A;Rapiejko, P;Der, K;Philbrick, K;Punreddy, S;Rigby, S;Smith, R;Feng, Q;Murrell, J;Rook, M; | Process development for expansion of human mesenchymal stromal cells in a 50L single-use stirred tank bioreactor | Biochemical Engineering Journal | PBMCs (AllCells, Alameda, CA) that had been thawed and incubated overnight in 3 mL RPMI medium in ultra-low attachment 6-well plates (Corning) were stained with CFDA-SE (Thermo Fisher Scientific), according to the manufacturer’s recommendations. For | Full Paper | |
2017 | Heng, BC;Gong, T;Wang, S;Lim, LW;Wu, W;Zhang, C; | Decellularized Matrix Derived from Neural Differentiation of Embryonic Stem Cells Enhances the Neurogenic Potential of Dental Follicle Stem Cells | Journal of endodontics | 28231979 | The hESCs (WA01 line) were obtained from Wicell Institute (Madison, WI). Human DFSCs (catalog no. FT002 F, lot no. 020609) were purchased from AllCells LLC (Alameda, CA). Unless mentioned otherwise, all cell culture media and supplements were procure | Full Paper |
2017 | Elwood, F;Witter, DJ;Piesvaux, J;Kraybill, B;Bays, N;Alpert, C;Goldenblatt, P;Qu, Y;Ivanovska, I;Lee, HH;Chiu, CS;Tang, H;Scott, ME;Deshmukh, SV;Zielstorff, M;Byford, A;Chakravarthy, K;Dorosh, L;Rivkin, A;Klappenbach, J;Pan, BS;Kariv, I;Dinsmore, C;Slipetz, D;Dandliker, PJ; | Evaluation of JAK3 Biology in Autoimmune Disease Using a Highly Selective, Irreversible JAK3 Inhibitor | The Journal of pharmacology and experimental therapeutics | 28193636 | Frozen, purified human CD34+ from mobilized blood (AllCells) were seeded at 100,000 cell/ml in Stempro34 complete medium (Thermo Fisher) supplemented with GMCSF at 20 ng/ml (Thermo Fisher), human stem cell factor at 40 ng/ml (Cell Signaling), and IL- | Full Paper |
2017 | Erickson, RI;Schutt, LK;Tarrant, JM;McDowell, M;Liu, L;Johnson, AR;Lewin-Koh, SC;Hedehus, M;Ross, J;Carano, RA;Staflin, K;Zhong, F;Crawford, JJ;Zhong, S;Reif, K;Katewa, A;Wong, H;Young, WB;Dambach, DM;Misner, DL; | Bruton's Tyrosine Kinase Small Molecule Inhibitors Induce a Distinct Pancreatic Toxicity in Rats | The Journal of pharmacology and experimental therapeutics | 27821712 | samples, sex unknown; Prodo Laboratories, Irvine, CA), universal RNA (rat/human; Clontech, Mountain View, CA), and total RNA from pooled isolated rat islet preparations (AllCells, Save | Full Paper |
2017 | Ye, J;Si, J;Cui, Z;Wang, Q;Peng, K;Chen, W;Peng, X;Chen, S; | Surface Modification of Electrospun TPU Nanofiber Scaffold with CNF Particles by Ultrasound-Assisted Technique for Tissue Engineering | Macromolecular Materials and Engineering | The cell viability of the TPU and CNF-adsorbed TPU nanofiber was determined using a MTT assay (3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide). Human umbilical vein endothelial cells (HUVECs, AllCells Biological Technology Co., Ltd., | Full Paper | |
2017 | McBride, JD;Rodriguez-Menocal, L;Guzman, W;Candanedo, A;Garcia-Contreras, M;Badiavas, EV; | Bone Marrow Mesenchymal Stem Cell-Derived CD63+ Exosomes Transport Wnt3a Exteriorly and Enhance Dermal Fibroblast Proliferation, Migration, and Angiogenesis In Vitro | Stem cells and development | 28679315 | BM-MSC "line 2" and "line 3" were obtained from healthy male and female donors, respectively, according to quality control and manufacturing established by AllCells , and isolated Save | Full Paper |
2017 | Setrerrahmane, S;Yu, J;Hao, J;Zheng, H;Xu, H; | Novel production method of innovative antiangiogenic and antitumor small peptides in Escherichia coli | Drug design, development and therapy | 29184391 | om GE Healthcare Life Sciences (Logan, UT, USA) and used for gene expression and protein purification, respectively. Human umbilical vein endothelial cells (HUVECs; primary cells) were purchased from AllCells (Shanghai, People’s Republic of China); a | Full Paper |
2017 | d Johnston, IC;Wagemaker, G; | development of a highly efficient lentiviral gene transfer protocol for hematopoietic stem cells | Book | Bone marrow samples were purchased from AllCells (Alameda, USA). These studies were performed according to established ethical guidelines, and all blood donors gave informed Save | Full Paper | |
2017 | Ito, R;Nagai, D;Igo, N;Okuda, Y;Sekine, K;Ichimura, E;Katano, I;Mizushima, T;Goto, M;Ohnishi, Y;Ito, M;Okamoto, K; | A novel in vivo model for predicting myelotoxicity of chemotherapeutic agents using IL-3/GM-CSF transgenic humanized mice | Toxicology letters | 28947270 | Commercially available human cord blood (CB)-derived CD34+ cells obtained from a single donor were purchased from Lonza (Basel, Switzerland) or Allcells (Alameda, CA, USA). The cells were prepared using a standard protocol for xenotransplantation | Full Paper |
2017 | FUKAHORI, H; | Investigation Regarding the Properties of Protein Kinase Cθ Selective Inhibitor as a Novel T cell Immunosuppressant | Thesis | T cell proliferation assay Human peripheral T cells were purchased from AllCells LLC (Alameda, CA, USA). Cells (1 x 105) suspended in RPMI 1640 containing 10% fetal bovine serum Save | Full Paper | |
2017 | Jin, J;Wang, Y;Xu, Y;Zhou, X;Liu, Y;Li, X;Wang, J; | MicroRNA-144 regulates cancer cell proliferation and cell-cycle transition in acute lymphoblastic leukemia through the interaction of FMN2 | The journal of gene medicine | 27556228 | ALL cell lines, Molt-3, Molt-4, Tall-104, Jurkat, CCRF-CEM, CEM/C1 and CEM/C2, were purchased from American Type Culture Collection (American Type Culture Collection, Manassas, VA, USA). A normal peripheral blood pan-T cell line (BPBPT) was purchased | Full Paper |
2017 | Heck, BE;Park, JJ;Makani, V;Kim, EC;Kim, DH; | PPAR-δ Agonist With Mesenchymal Stem Cells Induces Type II Collagen-Producing Chondrocytes in Human Arthritic Synovial Fluid | Cell transplantation | 28901183 | To eliminate donor-to-donor variability, we used synovial fluid from 3 different OA patients and used BM-MSCs from 3 donors (AllCells) to measure the GAG content. The use of human Save | Full Paper |
2017 | Bender, AT;Gardberg, A;Pereira, A;Johnson, T;Wu, Y;Grenningloh, R;Head, J;Morandi, F;Haselmayer, P;Liu-Bujalski, L; | Ability of Bruton's Tyrosine Kinase Inhibitors to Sequester Y551 and Prevent Phosphorylation Determines Potency for Inhibition of Fc Receptor but not B-Cell Receptor Signaling | Molecular pharmacology | 28062735 | E for 15 minutes and then measures CD63 upregulation by flow cytometry. Human cord blood isolated CD341 stem cells (AllCells, Alameda, CA) were differentiated to mast cells by culture for 8 weeks. Cells were maintained in StemPro-34 media (Gibco, Wal | Full Paper |
2017 | Fujisawa, K;Takami, T;Fukui, Y;Quintanilha, LF;Matsumoto, T;Yamamoto, N;Sakaida, I; | Evaluating effects of L-carnitine on human bone-marrow-derived mesenchymal stem cells | Cell and tissue research | 28197778 | The studied BmMSCs were bone marrow cells purchased from Allcells and were continuously cultured and used at passage 4 and passage 16. Flow cytometry results confirmed that Save | Full Paper |
2017 | Bai, L;Li, Q;Duo, X;Hao, X;Zhang, W;Shi, C;Guo, J;Ren, X;Feng, Y; | Electrospun PCL-PIBMD/SF blend scaffolds with plasmid complexes for endothelial cell proliferation | RSC Advances | 2.6.2. Cell culture.Human umbilical vein endothelial cells (HUVECs) were bought from Allcells Biomart (Shanghai, China) and cultured in Dulbecco's modified Eagle medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), 100 μg | Full Paper | |
2017 | Dong, B;Zhang, X;Fan, Y;Cao, S;Zhang, X; | GPR4 knockout improves renal ischemia-reperfusion injury and inhibits apoptosis via suppressing the expression of CHOP | The Biochemical journal | 29089376 | Cell culture and treatment Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCELLS (Emeryville, CA, U.S.A.). Cells were cultured in Dulbecco's modified Eagle medium (Gibco, U.S.A.) with 10% fetal bovine serum (Gibco, U.S.A.) at 3 | Full Paper |
2017 | Li, S;Yuan, H;Pan, J;Fan, W;Zhu, L;Yan, Z;Guo, C; | The treatment of femoral neck fracture using VEGF-loaded nanographene coated internal fixation screws | PloS one | 29117211 | The human umbilical vein endothelial cells (HUVECs) (Allcells, Alameda, CA, USA) and osteoblasts (Type culture collection of the Chinese Academy of Sciences, Shanghai, China) Save | Full Paper |
2017 | Sewing, S;Roth, AB;Winter, M;Dieckmann, A;Bertinetti-Lapatki, C;Tessier, Y;McGinnis, C;Huber, S;Koller, E;Ploix, C;Reed, JC;Singer, T;Rothfuss, A; | Assessing single-stranded oligonucleotide drug-induced effects in vitro reveals key risk factors for thrombocytopenia | PloS one | 29107969 | 0 sec after the end of the association phase in the binding sensorgram were evaluated to rank ONs. Human bone marrow toxicity assay Human mononucleated cells (hMNCs) were received cryopreserved from AllCells, LLC or STEMCELL Technologies Inc, and we | Full Paper |
2017 | Ross, SL;Sherman, M;McElroy, PL;Lofgren, JA;Moody, G;Baeuerle, PA;Coxon, A;Arvedson, T; | Bispecific T cell engager (BiTE®) antibody constructs can mediate bystander tumor cell killing | PloS one | 28837681 | Pan-T cells were purchased from AllCells. T cells that were not stimulated prior to use in assays are referred to as resting T cells. Activated T cells were generated by two methods: (1) Save | Full Paper |
2017 | Liu, L;Koo, Y;Collins, B;Xu, Z;Sankar, J;Yun, Y; | Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system | PloS one | 28797069 | The product was purchased from Allcells with the concentration of 5 x 10 8 /ml. PRP was used immediately upon arrival. Micro-Renathane tubing (0.066-in. Save | Full Paper |
2017 | Graci, JD;Michaels, D;Chen, G;Schiralli Lester, GM;Nodder, S;Weetall, M;Karp, GM;Gu, Z;Colacino, JM;Henderson, AJ; | Identification of benzazole compounds that induce HIV-1 transcription | PloS one | 28658263 | Brunswick, NJ (UMDNJ, now part of Rutgers University)[48]. ACH-2 and U1 cells were obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH. Cryopreserved PBMCs were purchased from AllCells, LLC (Alameda, CA). RPMI-1640, DMEM, hygr | Full Paper |
2017 | Salzberg, AC;Harris-Becker, A;Popova, EY;Keasey, N;Loughran, TP;Claxton, DF;Grigoryev, SA; | Genome-wide mapping of histone H3K9me2 in acute myeloid leukemia reveals large chromosomal domains associated with massive gene silencing and sites of genome instability | PloS one | 28301528 | Cryopreserved bone marrow CD34+ cells from normal donors were obtained from Allcells (Alameda, CA). Bone marrow CD34+ cells represent a combination of stem and progenitor Save | Full Paper |
2017 | Li, Y;He, Q;Hu, X;Liu, Y;Cheng, X;Li, X;Deng, F; | Improved performance of collagen scaffolds crosslinked by Traut's reagent and Sulfo-SMCC | Journal of biomaterials science. Polymer edition | 28277011 | The HUVECs used in this study were purchased from Allcells (Shanghai). HUVECs were cultured with endothelial complete medium (Allcells, Shanghai) in an incubator with 5% CO 2 Save | Full Paper |
2017 | Tharinger, H;Rebbapragada, I;Samuel, D;Novikov, N;Nguyen, MH;Jordan, R;Frey, CR;Pflanz, S; | Antibody-dependent and antibody-independent uptake of HBsAg across human leucocyte subsets is similar between individuals with chronic hepatitis B virus infection and healthy donors | Journal of viral hepatitis | 28012213 | Peripheral blood from healthy donors (HD) was purchased from AllCells (Alameda, CA, USA). Informed written consent was obtained from all enrolled study subjects prior to blood draw. Additional details related to subject enrolment can be found in supp | Full Paper |
2017 | Bonar, MM;Tilton, JC; | High sensitivity detection and sorting of infectious human immunodeficiency virus (HIV-1) particles by flow virometry | Virology | 28235684 | This study was conducted according to the principles specified in the Declaration of Helsinki and under local ethical guidelines (Case Western Reserve University Institutional Review Board (IRB)). Normal donor samples were de-identified and obtained | Full Paper |
2017 | Chen, S;Wu, X;Hu, J;Dai, G;Rong, A;Guo, G; | PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells | Molecular medicine reports | 28677750 | HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China). Cells were cultured in RPMI 1640 medium with 20% FBS, Save | Full Paper |
2017 | DiCarlo, AL;Tamarat, R;Rios, CI;Benderitter, M;Czarniecki, CW;Allio, TC;Macchiarini, F;Maidment, BW;Jourdain, JR; | Cellular Therapies for Treatment of Radiation Injury: Report from a NIH/NIAID and IRSN Workshop | Radiation research | 28605260 | having sources of ready-to-inject cells facilitates research. Although cells can be obtained from various commercial sources (e.g., Lonza, AllCells, Allosource), there is often variability in terms of how those cells grow in culture. There are govern | Full Paper |
2017 | Liu, X;Jiang, Y;Wu, J;Zhang, W;Liang, Y;Jia, L;Yu, J;Jeong, LS;Li, L; | NEDD8-activating enzyme inhibitor, MLN4924 (Pevonedistat) induces NOXA-dependent apoptosis through up-regulation of ATF-4 | Biochemical and biophysical research communications | 28450112 | HUVECs (Human umbilical vein endothelial cells) were obtained from Allcells Biotech Co. (Shanghai, China) and maintained in Dulbecco's Modified Eagle's Medium (GIBCO), containing 10% fetal bovine serum (FBS) and 1% Penicillin-Streptomycin solution, a | Full Paper |
2017 | Pittala, V;Vanella, L;Salerno, L;Di Giacomo, C;Acquaviva, R;Raffaele, M;Romeo, G;Modica, MN;Prezzavento, O;Sorrenti, V; | Novel Caffeic Acid Phenethyl Ester (Cape) Analogues as Inducers of Heme Oxygenase-1 | Current pharmaceutical design | 28190391 | Frozen hMSCs, derived from bone marrow, were purchased from Allcells (Emeryville, CA). After thawing, cells were resuspended in α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 20% heat inactivated fetal bovine serum (FB | Full Paper |
2017 | Chan, RC;Kotner, JS;Chuang, CM;Gaur, A; | Stabilization of pre-optimized multicolor antibody cocktails for flow cytometry applications | Cytometry. Part B, Clinical cytometry | 27001933 | RPMI 1640 medium (Hyclone), containing 10% fetal bovine serum (FBS), 2 mmol/L L-glutamine, and antibiotics. PBMCs used in long-term studies were stored in liquid nitrogen vapor. For some experiments, diseased and normal bone marrow samples were purch | Full Paper |
2017 | Dubey, N;Rajan, SS;Bello, YD;Min, KS;Rosa, V; | Graphene Nanosheets to Improve Physico-Mechanical Properties of Bioactive Calcium Silicate Cements | Materials (Basel, Switzerland) | 28772959 | tion, and Bioactivity The use of human monoradicular premolars and dental pulp stem cells (DPSC) for research was approved by the Institutional Review Board/NUS (Approval Number: NUS 2094). The DPSC (Allcells, Alameda, CA, USA) between passage 3 and | Full Paper |
2017 | Seyfried, TF;Gruber, M;Pawlik, MT;Kasper, S;Mandle, RJ;Hansen, E; | A new approach for fat removal in a discontinuous autotransfusion device-concept and evaluation | Vox sanguinis | 28960338 | Background Fat present during blood salvage in orthopaedic or cardiac surgery can pose a risk of fat embolism and should be eliminated before transfusion. Based on observations of Save | Full Paper |
2017 | Fei, T;Shao, Y;Yan, Z;Zhu, L;Li, S;Pan, J;Guo, C; | The effects of P-gp and CYP450 modulated by rifampicin on the steroid-induced osteonecrosis of the femoral head | Journal of bone and mineral metabolism | 27848009 | MSCs (RBM-006, ALLCELLS) were added to 24-well plates and cultured for three passages. The MSCs were incubated with blank medium, medium supplemented with dexamethasone (10 μmol/l), and dexamethasone (10 μmol/l) plus rifampicin (5 μg/ml), each treatm | Full Paper |
2017 | Mao, M;Zheng, X;Jin, B;Zhang, F;Zhu, L;Cui, L; | Effects of CD44 and E-cadherin overexpression on the proliferation, adhesion and invasion of ovarian cancer cells | Experimental and therapeutic medicine | 29285092 | CD44 is a prognostic indicator of shorter survival time in ovarian cancer. E‑cadherin fragmentation promotes the progression of ovarian cancer. However, the effects of CD44 and E‑ Save | Full Paper |
2017 | Sutou, S; | The 10th anniversary of the publication of genes and environment: memoir of establishing the Japanese environmental mutagen society and a proposal for a new collaborative study on mutagenic hormesis | Genes and environment : the official journal of the Japanese Environmental Mutagen Society | 28265305 | Some chemicals probably are mutagenic or carcinogenic to virtually allcells of all organisms. Among these would be the unstable chemical species such as alkylating agents and other Save | Full Paper |
2017 | He, L;Fan, F;Hou, X;Gao, C;Meng, L;Meng, S;Huang, S;Wu, H; | Resveratrol suppresses pulmonary tumor metastasis by inhibiting platelet-mediated angiogenic responses | The Journal of surgical research | 28629815 | Primary HUVECs were obtained from Allcells (Shanghai, China) and cultured in endothelial basal medium (Allcells) according to the manufacturer's protocol. Save | Full Paper |
2017 | Iwasa, M;Miura, Y;Fujishiro, A;Fujii, S;Sugino, N;Yoshioka, S;Yokota, A;Hishita, T;Hirai, H;Andoh, A;Ichinohe, T;Maekawa, T; | Bortezomib interferes with adhesion of B cell precursor acute lymphoblastic leukemia cells through SPARC up-regulation in human bone marrow mesenchymal stromal/stem cells | International journal of hematology | 28044259 | Human BM-MSCs were isolated from BM samples of healthy adults purchased from AllCells (Emeryville, CA, USA) based on our previously published method. A single-cell suspension Save | Full Paper |
2017 | Suknuntha, K;Thita, T;Togarrati, PP;Ratanachamnong, P;Wongtrakoongate, P;Srihirun, S;Slukvin, I;Hongeng, S; | Wnt signaling inhibitor FH535 selectively inhibits cell proliferation and potentiates imatinib-induced apoptosis in myeloid leukemia cell lines | International journal of hematology | 27766528 | Mononuclear cells from CML patient in chronic phase were procured from AllCells (CA, USA). Histopaque-1077 (Sigma-Aldrich) density gradient centrifugation was performed on total Save | Full Paper |
2017 | Hsieh, YT;Aggarwal, P;Cirelli, D;Gu, L;Surowy, T;Mozier, NM; | Characterization of FcγRIIIA effector cells used in in vitro ADCC bioassay: Comparison of primary NK cells with engineered NK-92 and Jurkat T cells | Journal of immunological methods | 27939300 | HemaCare (HemaCare Corporation, Van Nuys, CA) and AllCells (AllCells, LLC, Alameda, CA), respectively. Engineered NK-92 (V-158) and NK-92 (F-158) effector cell lines were purchased under a license agreement from Conkwest. The parent cells were transf | Full Paper |
2017 | Trang, K;Raposo, RA;Lowe, MM;Krow-Lucal, ER;Yonemoto, W;Cabido, VD;SenGupta, D;McCune, JM; | Relative mRNA Expression Levels of Restriction Factors and Antiviral Genes in Fetal and Adult Human Monocytes and Monocyte-Derived Macrophages | Viral immunology | 28151065 | ABM samples were obtained from healthy donors (AllCells, LLC or Lonza, Inc.). Both adult and fetal mononuclear cells were isolated by density centrifugation of a Ficoll-Hypaque Save | Full Paper |
2017 | Zhou, X;Liang, L;Zhao, Y;Zhang, H; | Epigallocatechin-3-Gallate Ameliorates Angiotensin II-Induced Oxidative Stress and Apoptosis in Human Umbilical Vein Endothelial Cells through the Activation of Nrf2/Caspase-3 Signaling | Journal of vascular research | 28942440 | HUVECs were obtained from Allcells Technology, China. The cells were seeded in RPMI 1640 medium with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 60 μg/mL of endothelial cell growth supplement (BD, San Diego, CA, USA) in an incubator at | Full Paper |
2017 | Goto, K;Goto, M;Ando-Imaoka, M;Kai, K;Mori, K; | Evaluation of drug-induced hematotoxicity using novel in vitro monkey CFU-GM and BFU-E colony assays | The Journal of toxicological sciences | 28717098 | Commercial leukopaks generally contain 80-200 mL of processed material with approximately 7x109peripheral blood mononuclear cells (PBMC), (more information available on: www.allcells.com/products/whole-tissue/leuko-pak). The Table I shows the percent | Full Paper |
2017 | Gao, X;Yourick, JJ;Sprando, RL; | Comparative transcriptomic analysis of endothelial progenitor cells derived from umbilical cord blood and adult peripheral blood: Implications for the generation of induced pluripotent stem cells | Stem cell research | 29156376 | Frozen human MNCs from umbilical CB and adult PB of healthy individuals were obtained from AllCells (Alameda, CA). The cells were thawed, washed, and resuspended in EGM-2 Save | Full Paper |
2017 | Enezei, H;Ahmad, A;Khamis, M;Suzuki, J;Sugita, Y;Maeda, H;Alshehadat, S;Razak, N;Abbas, S;Qabbani, A;Alam, M; | Enhanced Osteogenic and Angiogenic-Related Gene Expression of Human Dental Stem Cells on Biphasic Calcium Phosphate Scaffold Treated with Vascular Endothelial Growth Factor: Part I | Journal of Hard Tissue Biology | Cell culture: Commercially obtained DSCs from (AllCells, LLC Company, Emeryville, CA, USA) were cultured in the Dulbecco's Modifi ed Eagle Medium (DMEM) high glucose, (4.5g/l), Fetal bovine serum (FBS), Dulbecco's PBS (without Ca+2 and Mg+2), Penicil | Full Paper | |
2017 | Jasińska-Stroschein, M;Orszulak-Michalak, D; | Novel strategies for treatment of pulmonary arterial hypertension | Postepy higieny i medycyny doswiadczalnej (Online) | 28791952 | 2.3.2 EARLY OUTGROWTH EPC ISOLATION Human early EPCs were generated from leukapheresis samples obtained from Leuko-Paks purchased from AllCells, inc (Almeda, California, USA) or obtained from healthy donors in accordance with the Ottawa Hospital Rese | Full Paper |
2017 | Cheng, Z;Yi, Y;Xie, S;Yu, H;Peng, H;Zhang, G; | The effect of the JAK2 inhibitor TG101209 against T cell acute lymphoblastic leukemia (T-ALL) is mediated by inhibition of JAK-STAT signaling and activation of the crosstalk between apoptosis and autophagy signaling | Oncotarget | 29290986 | (A) HSD2, peer and bone marrow primary T-ALLcells were treated with increasing concentrations of TG101209 for 48 h, which was followed by analysis of apoptosis by staining with PI Save | Full Paper |
2017 | Chen, C;Li, H;Jiang, J;Zhang, Q;Yan, F; | Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats | Oncotarget | 29069818 | t have structural similarities to the human body to obtain more precise results. MATERIALS AND METHODS Cultivation of BMMSCs Bone marrow mesenchymal stem cell (BMMSCs) of SD rats were purchased from AllCells, LLC (Alameda, CA, USA), isolated and pur | Full Paper |
2017 | Brin, E;Wu, K;Lu, HT;He, Y;Dai, Z;He, W; | PEGylated arginine deiminase can modulate tumor immune microenvironment by affecting immune checkpoint expression, decreasing regulatory T cell accumulation and inducing tumor T cell infiltration | Oncotarget | 28938609 | richia coli. The recombinant protein is PEGylated with 20,000 MW PEG [40-42]. C397A substitution was used to generate the inactive ADI-PEG 20 mutant. Frozen healthy donor PBMCs were purchased from AllCells. Cancer cell lines AGS, A2780, A375, HCT116 | Full Paper |
2017 | Horn, LA;Ciavattone, NG;Atkinson, R;Woldergerima, N;Wolf, J;Clements, VK;Sinha, P;Poudel, M;Ostrand-Rosenberg, S; | CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1+ tumor cells, and extends the survival of tumor-bearing humanized mice | Oncotarget | 28938530 | PBMC used for NKT purification were obtained from AllCells, LLC (Alameda, CA) and were handled according to the manufacturer's direction. Fresh samples of blood from SCLC and Save | Full Paper |
2017 | Bilger, A;Plowshay, J;Ma, S;Nawandar, D;Barlow, EA;Romero-Masters, JC;Bristol, JA;Li, Z;Tsai, MH;Delecluse, HJ;Kenney, SC; | Leflunomide/teriflunomide inhibit Epstein-Barr virus (EBV)- induced lymphoproliferative disease and lytic viral replication | Oncotarget | 28574826 | id cell line (LCL) derived from the peripheral blood leukocytes of anonymous donors, obtained from the American Red Cross [55]. M81-Luc LCLs were obtained by infecting CD34-depleted human cord blood (AllCells) with M81-Luc virus, described below, and | Full Paper |
2017 | Estupina, P;Fontayne, A;Barret, JM;Kersual, N;Dubreuil, O;Le Blay, M;Pichard, A;Jarlier, M;Pugnière, M;Chauvin, M;Chardès, T;Pouget, JP;Deshayes, E;Rossignol, A;Abache, T;de Romeuf, C;Terrier, A;Verhaeghe, L;Gaucher, C;Prost, JF;Pèlegrin, A;Navarro-Teulon, I; | The anti-tumor efficacy of 3C23K, a glyco-engineered humanized anti-MISRII antibody, in an ovarian cancer model is mainly mediated by engagement of immune effector cells | Oncotarget | 28427157 | Ficoll procedure. Human and cynomolgus monkey PBMC were frozen in FBS with 10% DMSO and stored in liquid nitrogen until use. Murine PBMC isolated from C57BL/6 mice were directly purchased frozen from AllCells (Alameda, CA, USA). 51Cr-labeled COV434-M | Full Paper |
2017 | Diaz, MF;Evans, SM;Olson, SD;Cox, CS;Wenzel, PL; | A Co-culture Assay to Determine Efficacy of TNF-α Suppression by Biomechanically Induced Human Bone Marrow Mesenchymal Stem Cells | Bio-protocol | 28932763 | Human bone marrow (BM) MSC (Whole Bone Marrow aspirates) (AllCells, catalog number: ABM001-0) MSCs were isolated from whole bone marrow using a Ficoll gradient followed by plastic adherence and then cultured in MSC media (see Recipes) | Full Paper |
2017 | Noorani, TY;Luddin, N;Rahman, IA;Masudi, SM; | In Vitro Cytotoxicity Evaluation of Novel Nano-Hydroxyapatite-Silica Incorporated Glass Ionomer Cement | Journal of clinical and diagnostic research : JCDR | 28571275 | Mesenchymal stem cell (MSC) Basal Medium and supplements were purchased from ALLCELLS (USA). Tryp LE express was purchased from Invitrogen (California, USA). For MTT test, Save | Full Paper |
2017 | Wang, J;Zhang, Y;Zhu, Q;Liu, Y;Cheng, H;Zhang, Y;Li, T; | Data on the radioprotective effect of emodin in vivo and vitro via inhibition of apoptosis and modulation of p53 | Data in brief | 28275662 | aterials and methods 2.1 Experimental design and materials Male C57BL/6 mice were purchased from SIPPR-BK Experiment Animal Co. (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) from ALLCells, LLC (Emeryville, CA, USA). Emodin (purit | Full Paper |
2017 | Ahmed, H;Luddin, N;Kannan, T;Mokhtar, K;Ahmad, A; | Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells | European Journal of General Dentistry | molars (adult) and characterized was purchased from AllCells (MD, USA) and was used in (AllCells), with 50 ml of human mesenchymal stem cell stimulatory supplements (AllCells). Save | Full Paper | |
2017 | 张文博, ;张贤, ; | Influence of M2 macrophage supernatant combined with Eucommia flavonoids on the biological behavior of osteoblasts under hypoxic conditions | Chinese Journal of Tissue Engineering Research | : 文 快速阅读: 文题释义: M2 型巨噬细胞: 即替代活化巨噬细胞, 由白细胞介 4, 白细胞介 13 诱导, 可使甘露糖受体及转谷氨酰胺酶2 表达上调. M2 型巨噬细胞及其分泌 子可以促进组织 Save;METHODS:AfterMC3T3E1cellswereresuscitated,andM2macrophageswereisolated,inducedandcultured,allcells weredividedintocontrol,M2macrophage, Save | Full Paper | |
2017 | Richardson, C; | Mechanistic and Therapeutic Studies Related to Anemia of Chronic Disease and Inflammation | University of Virginia | follows: IFNγ 1500U/ml, TNFα 100ng/ml, IL-1β 100ng/ml, IL-6 100ng/ml IL-10 100ng/ml, IL-15 100ng/ml, and LPS 50ug/ml (PeproTech). Purified human CD36+ cells (AllCells LLC, Emeryville, CA) underwent expansion as previously described [73] for 48 hours | Full Paper | |
2017 | Alvey, C; | Engineered Marrow Macrophages For Cancer Therapy: Engorgement, Accumulation, Differentiation, And Acquired Immunity | Thesis | Human Specimens: Fresh human bone marrow was purchased from AllCells (Cat#: ABM001-1) in 3 mL quantities from healthy donors. AllCells received IRB approval from Alpha (Protocol #7000-SOP-046) which includes donor consent. In total, there were 4 diff | Full Paper | |
2017 | Walters, MC;Hongeng, S;Kwiatkowski, JL;Locatelli, F; | Results from the Hgb-207 (Northstar-2) Trial: A Phase 3 Study to Evaluate Safety and Efficacy of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia (TDT) in Patients with Non-β0/β0 Genotypes | Blood | oad : Download full-size image [https://ars.els-cdn.com/content/image/1-s2.0-S000649711981042X-gr1.jpg] DISCLOSURES WALTERS: _bluebird bio:_ Research Funding; _Sangamo Therapeutics:_ Consultancy; _AllCells, Inc:_ Other: Medical Director; _ViaCord | Full Paper | |
2017 | Talano, JA;Moore, TB;Keever-Taylor, CA;Shenoy, S; | Promising Results at 1 Year Follow-up Following Familial Haploidentical (FHI) T-Cell Depleted (TCD) with CD34 Enrichment and T-Cell (CD3) Addback Allogeneic Stem Cell Transplantation in Patients with High-Risk Sickle Cell Disease (SCD) (IND 14359) | Blood | ls-cdn.com/content/image/1-s2.0-S0006497119851182-gr1_lrg.jpg] * Download : Download full-size image [https://ars.els-cdn.com/content/image/1-s2.0-S0006497119851182-gr1.jpg] DISCLOSURES WALTERS: _AllCells, Inc:_ Other: Medical Director; _bluebird | Full Paper | |
2017 | Acharya, S; | Defining Immune Cell Profiles and their Role in Predicting Therapeutic Response in Multiple Myeloma | Thesis | Dana-Farber Cancer Institute. These samples were collected after informed consent in accordance with the Declaration of Helsinki and approved by the institutional review board of the Dana-Farber Cancer Institute. Healthy donor samples were obtained f | Full Paper | |
2017 | Accomando, WP;Wiencke, JK;Houseman, EA; | HousemanEugenePHHSQuantitativeReconstructionLeukocyte. pdf | Genome Biology | blood, that were utilized in this work. Blood was obtained through an institutional review board-approved donor program at AllCells, LLC (Alameda, CA, USA) and research was carried out in accordance with the Helsinki declaration. Samples were de-iden | Full Paper | |
2017 | Zhang, Y;Farhangfar, F;Giblin, L;Wolf, D;Vitelli, F; | Assessment of Differentiation States of Hematopoietic Stem Cells Following in Vitro Culture Using Side and Forward Scatter of Flow Cytometry | World Journal of Research and Review | G-CSF mobilized peripheral HSC (AllCells, LLC. Emeryville, CA) were cultured with CellGro®Stem Cell Expansion Media (CellGenix, Inc., Catalog number: 2001) containing 10ng/ml TPO (Peprotech, Inc. Rocky Hill, NJ. Catalog number: 300-18), 50ng/ml SCF | Full Paper | |
2017 | Gregg, KA; | Novel Lipid A Structures for Adjuvant Discovery and Development | Thesis | 100 IU/mL penicillin, 100 µg/mL streptomycin, 1 mM sodium pyruvate, and 200 mM l-glutamine. THP-1 (ATCC, Manassas, VA), primary mouse splenocytes, and primary human PBMCs (AllCells, LLC, Alameda, CA) were cultured in RPMI 1640 | Full Paper | |
2017 | Edun, FA; | Endothelial cell response to bioactive peptides and stiffened extracellular matrices: an investigation into atherogenic pathways | Thesis | example, samples delivered from AllCells (California, U.S.A.) had markedly different culture adherence and proliferation characteristics (cell doubling time) than HUVECs obtained from ThermoFisher, prompting our repeated purchase of only batch number | Full Paper | |
2017 | Bonar, MM; | Rapid Enumeration, Sorting and Maturation Analysis of Single Viral Particles in HIV-1 Swarms by High-resolution Flow Virometry | Thesis | identified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated by adding additional autologous red blood cells to leukapheresis samples and RosetteSep CD4+ T cell enrichment k | Full Paper | |
2017 | Basiorka, AA; | Changing the pathobiological paradigm in myelodysplastic syndromes: The NLRP3 inflammasome drives the MDS phenotype | Thesis | Normal, heparinized BM aspirates were purchased from Lonza Walkersville or AllCells, LLC. Normal and MDS BM mononuclear cells (BMMNC) were isolated from heparinized BM aspirates using Ficoll-Hypaque Plus gradient centrifugation (GE Healthcare). Recom | Full Paper | |
2017 | Bennett, LF; | Analysis of Stress Erythropoiesis during Inflammation: Stimulation of Toll-Like Receptors Induces Erythrophagocytosis and Activates Stress Erythropoiesis | Thesis | After 24 hours, cells were washed and replated with fresh SEEM. Human bone marrow mononuclear cells were purchased from ReachBio (0300-200) and cultured in SEEM containing human insulin, human holo-transferrin, and human cytokines. Human MNCs culture | Full Paper | |
2017 | Newkirk, SJ;Lee, S;Grandi, FC;Gaysinskaya, V;Rosser, JM;Vanden Berg, N;Hogarth, CA;Marchetto, MCN;Muotri, AR;Griswold, MD;Ye, P;Bortvin, A;Gage, FH;Boeke, JD;An, W; | Intact piRNA pathway prevents L1 mobilization in male meiosis | Proceedings of the National Academy of Sciences of the United States of America | 28630288 | wrote the paper. Reviewers: P.D., Tulane Cancer Center; and P.J.W., University of Pennsylvania. 1Present address: Cancer Biology Program, Stanford University, Stanford, CA 94305. 2Present address: ReachBio LLC, Seattle, WA 98107. 3Present address | Full Paper |
2017 | Zhen, A;Peterson, CW;Carrillo, MA;Reddy, SS;Youn, CS;Lam, BB;Chang, NY;Martin, HA;Rick, JW;Kim, J;Neel, NC;Rezek, VK;Kamata, M;Chen, ISY;Zack, JA;Kiem, HP;Kitchen, SG; | Long-term persistence and function of hematopoietic stem cell-derived chimeric antigen receptor T cells in a nonhuman primate model of HIV/AIDS | PLoS pathogens | 29284044 | Following conditioning, the HSPC product was infused back into the autologous animal. A small aliquot of the infused cell product was plated in Colony Gel Medium (Reach Bio, Seattle, WA) and analyzed as previously described[14,39]. Individual colonie | Full Paper |
2017 | Liu, FD;Pishesha, N;Poon, Z;Kaushik, T;Van Vliet, KJ; | Material Viscoelastic Properties Modulate the Mesenchymal Stem Cell Secretome for Applications in Hematopoietic Recovery | ACS biomaterials science & engineering | 33445371 | Human bone marrow-derived MSCs (MSCs) were purchased from multiple commercial sources (Lonza, RoosterBio, and ReachBio) at what was stated by the vendors to be a low Save | Full Paper |
2017 | Hinerfeld, D;Fuhrman, K;Demirkan, G;DosSantos, G;Geiss, G; | A simple and integrated workflow for deep proteomic and transcriptomic analysis of sorted cell populations | The Journal of Immunology | 1Nanostring Technologies++1Nanostring Technologies++1Nanostring Technologies++2Reachbio Research Labs++1Nanostring Technologies | Full Paper | |
2017 | Min, C;Moore, N;Shearstone, JR;Quayle, SN;Huang, P;van Duzer, JH;Jarpe, MB;Jones, SS;Yang, M; | Selective Inhibitors of Histone Deacetylases 1 and 2 Synergize with Azacitidine in Acute Myeloid Leukemia | PloS one | 28060870 | Specifically, compounds were dissolved in DMSO and added to tubes of methylcellulose-based medium (Reachbio, catalog 1103) containing cytokine formulations to support colony Save;row samples from AML patients were treated with ACY-957 alone, azacit | Full Paper |
2017 | Kudrimoti, M;Curtis, A;Azawi, S;Worden, F;Katz, S;Adkins, D;Bonomi, M;Scott, Z;Elder, J;Sonis, ST;Straube, R;Donini, O; | Dusquetide: Reduction in oral mucositis associated with enduring ancillary benefits in tumor resolution and decreased mortality in head and neck cancer patients | Biotechnology reports (Amsterdam, Netherlands) | 28649557 | 1).Fig. 1Dusquetide Pre-Incubated with Stromal Cells inhibits the Future Growth of Co-cultured Multiple Myeloma Cells. Previously defined primary human bone marrow stromal (mesenchymal (MSC)) cells (ReachBio lot# 2221207) were plated at a concentrati | Full Paper |
2017 | Bennett, LF; | Analysis of Stress Erythropoiesis: Stimulation of Toll-Like Receptors Induces Erythrophagocytosis and Activates Stress Erythropoiesis | Thesis | After 24 hours, cells were washed and replated with fresh SEEM. Human bone marrow mononuclear cells were purchased from ReachBio (0300-200) and cultured in SEEM containing human insulin, human holo-transferrin, and human cytokines. Human MNCs culture | Full Paper | |
2017 | Bogeholz, JL; | CD 33 in der akuten melodischen Leukämie | Thesis | ColonyGel 1101 ReachBio, Seattle, WA Steriles Wasser Helmholtzzentrum Fc-Block (FcR Blocking Reagent) Miltenyi Biotec, Bergisch Gladbach | Full Paper | |
2016 | Goldberg, SB;Gettinger, SN;Mahajan, A;Chiang, AC;Herbst, RS;Sznol, M;Tsiouris, AJ;Cohen, J;Vortmeyer, A;Jilaveanu, L;Yu, J;Hegde, U;Speaker, S;Madura, M;Ralabate, A;Rivera, A;Rowen, E;Gerrish, H;Yao, X;Chiang, V;Kluger, HM; | Pembrolizumab for patients with melanoma or non-small-cell lung cancer and untreated brain metastases: early analysis of a non-randomised, open-label, phase 2 trial | Lancet Oncol. | 27267608 | In patients with NSCLC, PD-L1 staining was done by Qualtek (Goleta, CA, USA) on tissue from any disease site after the most recent systemic therapy employing previously described methods 6 using 1% staining as a cutpoint for positivity | Full Paper |
2016 | Seiwert, TY;Burtness, B;Mehra, R;Weiss, J;Berger, R;Eder, JP;Heath, K;McClanahan, T;Lunceford, J;Gause, C;Cheng, JD;Chow, LQ; | Safety and clinical activity of pembrolizumab for treatment of recurrent or metastatic squamous cell carcinoma of the head and neck (KEYNOTE-012): an open-label, multicentre, phase 1b trial | Lancet Oncol. | 27247226 | Funding for this research was provided by Merck & Co, Inc, Kenilworth, NJ, USA. The authors thank the patients and their families and caregivers for participating in the study. The authors also thank Mark Ayers, Michael Nebozhyn, Erin Murphy, and Jen | Full Paper |
2016 | Muro, K;Chung, HC;Shankaran, V;Geva, R;Catenacci, D;Gupta, S;Eder, JP;Golan, T;Le, DT;Burtness, B;McRee, AJ;Lin, CC;Pathiraja, K;Lunceford, J;Emancipator, K;Juco, J;Koshiji, M;Bang, YJ; | Pembrolizumab for patients with PD-L1-positive advanced gastric cancer (KEYNOTE-012): a multicentre, open-label, phase 1b trial | Lancet Oncol. | 27157491 | To screen for PD-L1 expression at enrolment, archival tumour samples were assessed with a laboratory-developed prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA, USA), which included the 22C3 antibody (Merck & Co, Kenil | Full Paper |
2016 | Snyder, MW;Kircher, M;Hill, AJ;Daza, RM;Shendure, J; | Cell-free DNA Comprises an In Vivo Nucleosome Footprint that Informs Its Tissues-Of-Origin | Cell | 26771485 | ... man peripheral blood plasma from healthy donors, donors with clinical diagnosis of Stage IV cancer, and donors with clinical diagnosis with autoimmune disease (Tables S1, S4 and S5) was obtained from Conversant Bio (Huntsville, Alabama, USA) or P | Full Paper |
2016 | Armand, P;Shipp, MA;Ribrag, V;Michot, JM;Zinzani, PL;Kuruvilla, J;Snyder, ES;Ricart, AD;Balakumaran, A;Rose, S;Moskowitz, CH; | Programmed Death-1 Blockade With Pembrolizumab in Patients With Classical Hodgkin Lymphoma After Brentuximab Vedotin Failure | J. Clin. Oncol. | 27354476 | PD-L1 expression was determined using either fresh or archival formalin-fixed, paraffin-embedded tissue sectioned at 4 to 5 microns, with a proprietary assay developed at QualTek Molecular Laboratories (Newtown, PA) in collaboration with Merck (Kenil | Full Paper |
2016 | Nanda, R;Chow, LQ;Dees, EC;Berger, R;Gupta, S;Geva, R;Pusztai, L;Pathiraja, K;Aktan, G;Cheng, JD;Karantza, V;Buisseret, L; | Pembrolizumab in Patients With Advanced Triple-Negative Breast Cancer: Phase Ib KEYNOTE-012 Study | J. Clin. Oncol. | 27138582 | Filhart and Kenneth Emancipator (Merck & Co., Kenilworth, NJ) for immunohistochemistry expertise and study support, Roger Dansey (Merck & Co.) for critical manuscript review and study support, Karl Heath (Merck & Co.) for study support, and QualTek M | Full Paper |
2016 | Imsland, F;McGowan, K;Rubin, CJ;Henegar, C;Sundström, E;Berglund, J;Schwochow, D;Gustafson, U;Imsland, P;Lindblad-Toh, K;Lindgren, G;Mikko, S;Millon, L;Wade, C;Schubert, M;Orlando, L;Penedo, MC;Barsh, GS;Andersson, L; | Regulatory mutations in TBX3 disrupt asymmetric hair pigmentation that underlies Dun camouflage color in horses | Nat. Genet. | 26691985 | Science for Life Laboratory Uppsala, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, USA.++Science for Life Laboratory Uppsala, Department of Med | Full Paper |
2016 | Wang, G;Lu, X;Dey, P;Deng, P;Wu, CC;Jiang, S;Fang, Z;Zhao, K;Konaparthi, R;Hua, S;Zhang, J;Li-Ning-Tapia, EM;Kapoor, A;Wu, CJ;Patel, NB;Guo, Z;Ramamoorthy, V;Tieu, TN;Heffernan, T;Zhao, D;Shang, X;Khadka, S;Hou, P;Hu, B;Jin, EJ;Yao, W;Pan, X;Ding, Z;Shi, Y;Li, L;Chang, Q;Troncoso, P;Logothetis, CJ;McArthur, MJ;Chin, L;Wang, YA;DePinho, RA; | Targeting YAP-Dependent MDSC Infiltration Impairs Tumor Progression | Cancer Discov | 26701088 | ... d R&D Biosystems (Minneaplois, MN). Cxcr2 antibody was obtained from Bioss and R & D Biosystems. CD45 and Ly-6G antibodies were obtained from Biolegend. Prostate tissue micrcroarray was obtained from Folio Bioscience. CHROMATIN IMMUNOPRECIPITATI | Full Paper |
2016 | Vilgelm, AE;Johnson, CA;Prasad, N;Yang, J;Chen, SC;Ayers, GD;Pawlikowski, JS;Raman, D;Sosman, JA;Kelley, M;Ecsedy, JA;Shyr, Y;Levy, SE;Richmond, A; | Connecting the Dots: Therapy-Induced Senescence and a Tumor-Suppressive Immune Microenvironment | J. Natl. Cancer Inst. | 26719346 | Tennessee Valley Healthcare System, Department of Veterans Affairs, Nashville, TN (AEV, CAJ, JY, JSP, DR, AR); Department of Cancer Biology (AEV, AJ, JY, SCC, JSP, DR, AR), Center for Quantitative Sciences (SCC), Division of Cancer Biostatistics, Dep | Full Paper |
2016 | Sun, JM;Zhou, W;Choi, YL;Choi, SJ;Kim, SE;Wang, Z;Dolled-Filhart, M;Emancipator, K;Wu, D;Weiner, R;Frisman, D;Kim, HK;Choi, YS;Shim, YM;Kim, J; | Prognostic Significance of PD-L1 in Patients with Non-Small Cell Lung Cancer: A Large Cohort Study of Surgically Resected Cases | J Thorac Oncol | 27103510 | Automated staining (QualTek TekMate 500, QualTek Molecular Laboratories, Newtown, PA) included rinses with 1× wash buffer (#K8012 Envision FLEX+ kit, Dako), 10-minute proteinase K incubation (#S3020, Dako), 1× wash buffer rinse, and peroxidase blocki | Full Paper |
2016 | Wakeman, CA;Moore, JL;Noto, MJ;Zhang, Y;Singleton, MD;Prentice, BM;Gilston, BA;Doster, RS;Gaddy, JA;Chazin, WJ;Caprioli, RM;Skaar, EP; | The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction | Nat Commun | 27301800 | Total RNA was harvested using a combination of LETS buffer (0.1 M LiCl; 10 mM EDTA; 10 mM Tris HCl pH 7.4; 1% SDS) and TRI Reagent as previously described61. qRT–PCR was performed using SYBR green supermix (Bio-Rad) following manufacturer’s instructi | Full Paper |
2016 | Yang, Z;Shah, K;Khodadadi-Jamayran, A;Jiang, H; | Dpy30 is critical for maintaining the identity and function of adult hematopoietic stem cells | J. Exp. Med. | 27647347 | Total RNAs and ChIP (and their corresponding input) DNAs were sent to the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology (Huntsville, AL) for sequencing, including library construction. In brief, the quality of the total RNA and | Full Paper |
2016 | Resendez, SL;Jennings, JH;Ung, RL;Namboodiri, VM;Zhou, ZC;Otis, JM;Nomura, H;McHenry, JA;Kosyk, O;Stuber, GD; | Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses | Nat Protoc | 26914316 | Dust-off compressed gas (Falcon Safety Products, DPSXL) Gelfoam absorbable sponge (Pfizer Injectables, cat. no. 00009-0342-01) 12-well culture plates (Falcon, cat. no. 353043) Skull screws (Glass and Watch Screws, 900 piece set, JT69900) 1/16-inc | Full Paper |
2016 | Amendola, LM;Jarvik, GP;Leo, MC;McLaughlin, HM;Akkari, Y;Amaral, MD;Berg, JS;Biswas, S;Bowling, KM;Conlin, LK;Cooper, GM;Dorschner, MO;Dulik, MC;Ghazani, AA;Ghosh, R;Green, RC;Hart, R;Horton, C;Johnston, JJ;Lebo, MS;Milosavljevic, A;Ou, J;Pak, CM;Patel, RY;Punj, S;Richards, CS;Salama, J;Strande, NT;Yang, Y;Plon, SE;Biesecker, LG;Rehm, HL; | Performance of ACMG-AMP Variant-Interpretation Guidelines among Nine Laboratories in the Clinical Sequencing Exploratory Research Consortium | Am. J. Hum. Genet. | 27181684 | Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA.++Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA. Electronic address: pair@u.washington.edu | Full Paper |
2016 | Green, RC;Goddard, KAB;Jarvik, GP;Amendola, LM;Appelbaum, PS;Berg, JS;Bernhardt, BA;Biesecker, LG;Biswas, S;Blout, CL;Bowling, KM;Brothers, KB;Burke, W;Caga-Anan, CF;Chinnaiyan, AM;Chung, WK;Clayton, EW;Cooper, GM;East, K;Evans, JP;Fullerton, SM;Garraway, LA;Garrett, JR;Gray, SW;Henderson, GE;Hindorff, LA;Holm, IA;Lewis, MH;Hutter, CM;Janne, PA;Joffe, S;Kaufman, D;Knoppers, BM;Koenig, BA;Krantz, ID;Manolio, TA;McCullough, L;McEwen, J;McGuire, A;Muzny, D;Myers, RM;Nickerson, DA;Ou, J;Parsons, DW;Petersen, GM;Plon, SE;Rehm, HL;Roberts, JS;Robinson, D;Salama, JS;Scollon, S;Sharp, RR;Shirts, B;Spinner, NB;Tabor, HK;Tarczy-Hornoch, P;Veenstra, DL;Wagle, N;Weck, K;Wilfond, BS;Wilhelmsen, K;Wolf, SM;Wynn, J;Yu, JH;, ; | Clinical Sequencing Exploratory Research Consortium: Accelerating Evidence-Based Practice of Genomic Medicine | Am. J. Hum. Genet. | 27181682 | Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Medical School, Boston, MA 02115, USA; Partners Personalized Medicine, Boston, MA | Full Paper |
2016 | Lieberman, LA;Zeng, W;Singh, C;Wang, W;Otipoby, KL;Loh, C;Plavina, T;Gorelik, L;Ransohoff, RM;Cahir-McFarland, E; | CD62L is not a reliable biomarker for predicting PML risk in natalizumab-treated R-MS patients | Neurology | 26718566 | Thirty-four frozen PBMC samples were purchased from Conversant Bio (Huntsville, AL) to test the effects of other medical conditions on the % CD62L determination. Twenty-one matched healthy donor samples were collected. Eight samples were collected fr | Full Paper |
2016 | Hu, X;Liu, X;Moisan, J;Wang, Y;Lesch, CA;Spooner, C;Morgan, RW;Zawidzka, EM;Mertz, D;Bousley, D;Majchrzak, K;Kryczek, I;Taylor, C;Van Huis, C;Skalitzky, D;Hurd, A;Aicher, TD;Toogood, PL;Glick, GD;Paulos, CM;Zou, W;Carter, LL; | Synthetic RORγ agonists regulate multiple pathways to enhance antitumor immunity | Oncoimmunology | 28123897 | ... ion. After 5 days, cytokine levels in the media were determined using a luminex panel (R&D Systems). Cells were collected for flow cytometry analysis. PBMCs from cancer patients were purchased from Conversant Bio and activated with anti-CD3/28 b | Full Paper |
2016 | Cataisson, C;Michalowski, AM;Shibuya, K;Ryscavage, A;Klosterman, M;Wright, L;Dubois, W;Liu, F;Zhuang, A;Rodrigues, KB;Hoover, S;Dwyer, J;Simpson, MR;Merlino, G;Yuspa, SH; | MET signaling in keratinocytes activates EGFR and initiates squamous carcinogenesis | Sci Signal | 27330189 | This procedure was performed by a commercial vendor (Phylogeny, Inc.) according to the published method (88). FFPE TMAs from US Biomax were deparaffinized for 5 minutes in xylene, immersed in 100% ethanol for 5 minutes then air-dried. Treatment was w | Full Paper |
2016 | Bryant, SA;Herdy, JR;Amemiya, CT;Smith, JJ; | Characterization of Somatically-Eliminated Genes During Development of the Sea Lamprey (Petromyzon marinus) | Mol. Biol. Evol. | 27288344 | Total RNA was isolated from lamprey embryos using Trizol extraction. RNA quality was assessed on the Agilent 2100 Bioanalyzer (Agilent Technologies) and samples with RNA Integrity Number (RIN) 8 were sent to the HudsonAlpha Genomic Services Lab (Huds | Full Paper |
2016 | Jiang, L;Huang, J;Higgs, BW;Hu, Z;Xiao, Z;Yao, X;Conley, S;Zhong, H;Liu, Z;Brohawn, P;Shen, D;Wu, S;Ge, X;Jiang, Y;Zhao, Y;Lou, Y;Morehouse, C;Zhu, W;Sebastian, Y;Czapiga, M;Oganesyan, V;Fu, H;Niu, Y;Zhang, W;Streicher, K;Tice, D;Zhao, H;Zhu, M;Xu, L;Herbst, R;Su, X;Gu, Y;Li, S;Huang, L;Gu, J;Han, B;Jallal, B;Shen, H;Yao, Y; | Genomic Landscape Survey Identifies SRSF1 as a Key Oncodriver in Small Cell Lung Cancer | PLoS Genet. | 27093186 | ... s >70%; the tumor content in each NAT was< 3%. The Caucasian SCLC patient cohort consisted of 25 FFPE lung tumor tissue specimens with matched normal adjacent tissue pairs, which were purchased from Conversant Biologics, Inc (Huntsville, AL) (S1 | Full Paper |
2016 | Oksenberg, D;Dufu, K;Patel, MP;Chuang, C;Li, Z;Xu, Q;Silva-Garcia, A;Zhou, C;Hutchaleelaha, A;Patskovska, L;Patskovsky, Y;Almo, SC;Sinha, U;Metcalf, BW;Archer, DR; | GBT440 increases haemoglobin oxygen affinity, reduces sickling and prolongs RBC half-life in a murine model of sickle cell disease | Br. J. Haematol. | 27378309 | USA (IRB 88‐034)]. Blood from healthy donors was obtained from the Stanford Blood Center (Stanford, CA, USA) and umbilical cord blood was obtained from Conversant Bio, Inc. (Huntsville, AL, USA). The anti‐coagulant was | Full Paper |
2016 | Kirby, MK;Ramaker, RC;Gertz, J;Davis, NS;Johnston, BE;Oliver, PG;Sexton, KC;Greeno, EW;Christein, JD;Heslin, MJ;Posey, JA;Grizzle, WE;Vickers, SM;Buchsbaum, DJ;Cooper, SJ;Myers, RM; | RNA sequencing of pancreatic adenocarcinoma tumors yields novel expression patterns associated with long-term survival and reveals a role for ANGPTL4 | Mol Oncol | 27282075 | ... wling for critical reading of the manuscript. We thank Dr. Nick Cochran for packaging the _ANGPTL4_ lentiviral vectors. We thank Dr. Shawn Levy, Braden Boone, Angela Jones, and all the members of the HudsonAlpha Genomic Services Lab for providing | Full Paper |
2016 | O'Shannessy, DJ;Smith, MF;Somers, EB;Jackson, SM;Albone, E;Tomkowicz, B;Cheng, X;Park, Y;Fernando, D;Milinichik, A;Kline, B;Fulton, R;Oberoi, P;Nicolaides, NC; | Novel antibody probes for the characterization of endosialin/TEM-1 | Oncotarget | 27494870 | ... btained from various commercial vendors with Institutional Review Board approvals and patient consent and were collected between 2009 and 2011 (Bioreclamation, Westbury, NY; Indivumed, Lewisburg, PA; Folio Biosciences, Columbus, OH; Discovery Lif | Full Paper |
2016 | Ladd, B;Mazzola, AM;Bihani, T;Lai, Z;Bradford, J;Collins, M;Barry, E;Goeppert, AU;Weir, HM;Hearne, K;Renshaw, JG;Mohseni, M;Hurt, E;Jalla, S;Bao, H;Hollingsworth, R;Reimer, C;Zinda, M;Fawell, S;D'Cruz, CM; | Effective combination therapies in preclinical endocrine resistant breast cancer models harboring ER mutations | Oncotarget | 27472462 | ... ired ER mutations. MATERIALS AND METHODS Generation of the CTX model/efficacy studies Blood samples from MBC patients, consented according to the Human Biological Samples Policy, were purchased from Conversant Biologics. Due to patient confident | Full Paper |
2016 | Bradford, JR;Wappett, M;Beran, G;Logie, A;Delpuech, O;Brown, H;Boros, J;Camp, NJ;McEwen, R;Mazzola, AM;D'Cruz, C;Barry, ST; | Whole transcriptome profiling of patient-derived xenograft models as a tool to identify both tumor and stromal specific biomarkers | Oncotarget | 26980748 | ... subcutaneously into either NSG or CB17 SCID mice. The HBXF-079 sample was implanted orthotopically. The CTC-174 model was developed from circulating tumor cells in patient blood samples procured from Conversant Biologics (Huntsville, AL) and cons | Full Paper |
2016 | Malek, E;Driscoll, JJ; | High throughput chemical library screening identifies a novel p110-δ inhibitor that potentiates the anti-myeloma effect of bortezomib | Oncotarget | ... om Selleck Chemicals (Houston, TX). Human bone marrow stromal cell line HS-5 (ATCC) was cultured in DMEM. Patient bone marrow aspirates were obtained after Institutional Review Board approval or from ConversantBio (Huntsville, AL) and CD138+ cell | Full Paper | |
2016 | Aeffner, F;Martin, NT;Peljto, M;Black, JC;Major, JK;Jangani, M;Ports, MO;Krueger, JS;Young, GD; | Quantitative assessment of pancreatic cancer precursor lesions in IHC-stained tissue with a tissue image analysis platform | Lab. Invest. | 27775692 | tissue was evaluated. Materials and methods. Human PDAC Tissue. IRB-approved, formalin-fixed tumor samples from pancreatic cancer patients were provided by Conversant Bio (Huntsville, AL, USA). Animals. Kras LSL-G12D | Full Paper |
2016 | Haskett, S;Ding, J;Zhang, W;Thai, A;Cullen, P;Xu, S;Petersen, B;Kuznetsov, G;Jandreski, L;Hamann, S;Reynolds, TL;Allaire, N;Zheng, TS;Mingueneau, M; | Identification of Novel CD4+ T Cell Subsets in the Target Tissue of Sjögren's Syndrome and Their Differential Regulation by the Lymphotoxin/LIGHT Signaling Axis | J. Immunol. | 27815440 | Blocks of formalin-fixed tissue biopsies were acquired from Folio Biosciences (Powell, OH). Salivary tissues included submandibular glands (n = 2), parotid glands (n = 1), and labial glands (n = 3) from Sjo¨gren’s patients. Diagnoses were consistent | Full Paper |
2016 | Yoder, KE;Bundschuh, R; | Host Double Strand Break Repair Generates HIV-1 Strains Resistant to CRISPR/Cas9 | Sci Rep | 27404981 | HIV-1 infections were continued for 21 days in 4 replicates per cell type and virus strain. The virus supernatant was passaged to uninfected CRISPR cells. Infection was visually confirmed by the presence of syncytia. Two weeks later the genomic DNA w | Full Paper |
2016 | Dolled-Filhart, M;Locke, D;Murphy, T;Lynch, F;Yearley, JH;Frisman, D;Pierce, R;Weiner, R;Wu, D;Emancipator, K; | Development of a Prototype Immunohistochemistry Assay to Measure Programmed Death Ligand-1 Expression in Tumor Tissue | Arch. Pathol. Lab. Med. | 27788043 | Merck & Co, Inc, Kenilworth, New Jersey; the Departments of Early Clinical & Translational Research, Clinical Histochemistry (Dr Locke), Research and Clinical Client Services (Dr Murphy), and Operations and Client Services (Dr Lynch), QualTek Molecul | Full Paper |
2016 | Dolled-Filhart, M;Roach, C;Toland, G;Stanforth, D;Jansson, M;Lubiniecki, GM;Ponto, G;Emancipator, K; | Development of a Companion Diagnostic for Pembrolizumab in Non-Small Cell Lung Cancer Using Immunohistochemistry for Programmed Death Ligand-1 | Arch. Pathol. Lab. Med. | 27552095 | The prototype PD-L1 IHC assay was used to determine enrollment of patients in KEYNOTE-001 and was performed at a single laboratory site at QualTek (Goleta, California), which is a College of American Pathologists/Clinical Laboratory Improvement Amend | Full Paper |
2016 | Durning, SP;Flanagan-Steet, H;Prasad, N;Wells, L; | O-Linked β-N-acetylglucosamine (O-GlcNAc) Acts as a Glucose Sensor to Epigenetically Regulate the Insulin Gene in Pancreatic Beta Cells | J. Biol. Chem. | 26598517 | samples after the previously described 1-h incubation. Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL) for RNA-Seq library prep and sequencing. Briefly, the concentration and integrity of the | Full Paper |
2016 | VonDran, M;Thomas, JA;Freund, MP;Ritsick, M;Orr, M;Kaye, WE;Bakker, A;Knight, P; | The National Disease Research Interchange and Collaborators on: What Are the Major Hurdles to the Recovery of Human Tissue to Advance Research? | Biopreserv Biobank | 27845557 | The Foundation has collected more than 100 cutaneous neurofibromas along with patient-reported NF medical histories. Both tissue and data are stored at Precision Bioservices (Frederick, MD). Full “omic” characterization of 50 samples was outsourced t | Full Paper |
2016 | Chisamore, MJ;Gentile, MA;Dillon, GM;Baran, M;Gambone, C;Riley, S;Schmidt, A;Flores, O;Wilkinson, H;Alves, SE; | A novel selective androgen receptor modulator (SARM) MK-4541 exerts anti-androgenic activity in the prostate cancer xenograft R-3327G and anabolic activity on skeletal muscle mass & function in castrated mice | J. Steroid Biochem. Mol. Biol. | 27106747 | Excised tumor samples were immediately fixed with 10% buffered formalin, and sent to QualTek (Santa Barbara, CA) where tumors were embedded in paraffin, 4-μm thick sections were mounted on charged slides, dried at 60 °C for one hour, and deparaffinze | Full Paper |
2016 | Qadota, H;Mayans, O;Matsunaga, Y;McMurry, JL;Wilson, KJ;Kwon, GE;Stanford, R;Deehan, K;Tinley, TL;Ngwa, VM;Benian, GM; | The SH3 domain of UNC-89 (obscurin) interacts with paramyosin, a coiled-coil protein, in Caenorhabditis elegans muscle | Mol. Biol. Cell | 27009202 | Genomic DNA from each strain was prepared and provided to the Emory Integrated Genomics Core (EIGC) for Illumina sequencing. The Genomic Services Laboratory at the HudsonAlpha Institute for Biotechnology (Huntsville, AL) performed quality control and | Full Paper |
2016 | Slamecka, J;Salimova, L;McClellan, S;van Kelle, M;Kehl, D;Laurini, J;Cinelli, P;Owen, L;Hoerstrup, SP;Weber, B; | Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions | Cell Cycle | 26654216 | RNA was extracted from iPSC cultures using RNeasy Plus Mini kit (74134, Qiagen, https://www.qiagen.com/us/shop/sample-technologies/rna-sample-technologies/total-rna/rneasy-plus-micro-and-mini-kits). Whole genome expression analysis was performed by t | Full Paper |
2016 | Aycock, KI;Campbell, RL;Lynch, FC;Manning, KB;Craven, BA; | The Importance of Hemorheology and Patient Anatomy on the Hemodynamics in the Inferior Vena Cava | Ann Biomed Eng | 27272211 | Full Paper | |
2016 | Rahman, KM;Camp, ME;Prasad, N;McNeel, AK;Levy, SE;Bartol, FF;Bagnell, CA; | Age and Nursing Affect the Neonatal Porcine Uterine Transcriptome | Biol. Reprod. | 26632611 | United States Department of Agriculture, Agriculture Research Service, Clay Center, Nebraska Department of Animal Sciences, Endocrinology and Animal Biosciences Program, Rutgers University, New Brunswick, New Jersey.++Department of Animal Sciences, E | Full Paper |
2016 | Havird, JC;Santos, SR; | Developmental Transcriptomics of the Hawaiian Anchialine Shrimp Halocaridina rubra Holthuis, 1963 (Crustacea: Atyidae) | Integr. Comp. Biol. | 27400978 | RNA fragmentation, first-strand cDNA synthesis and amplification, adapter and barcode addition, and size selection were then performed according to Meyer et al. (2011) using the step-by-step protocol at http://people.oregonstate.edu/∼meyere/tools.htm | Full Paper |
2016 | Sorensen, SF;Zhou, W;Dolled-Filhart, M;Georgsen, JB;Wang, Z;Emancipator, K;Wu, D;Busch-Sørensen, M;Meldgaard, P;Hager, H; | PD-L1 Expression and Survival among Patients with Advanced Non-Small Cell Lung Cancer Treated with Chemotherapy | Transl Oncol | 26947883 | gen retrieval, along with a 10-minute proteinase K incubation (#S3020, Dako) following a 1 × wash buffer rinse (#K8012 Envision FLEX + kit, Dako) as part of automated staining. Automated staining (QualTek TekMate 500, Newtown, PA) was followed by per | Full Paper |
2016 | Zea, L;Prasad, N;Levy, SE;Stodieck, L;Jones, A;Shrestha, S;Klaus, D; | A Molecular Genetic Basis Explaining Altered Bacterial Behavior in Space | PLoS ONE | 27806055 | BioServe Space Technologies, Aerospace Engineering Sciences Dept., University of Colorado, Boulder, CO, United States of America.++Genomic Services Laboratory, HudsonAlpha Institute for Biotechnology, Huntsville, AL, United States of America.++Genomi | Full Paper |
2016 | McCoy, CR;Rana, S;Stringfellow, SA;Day, JJ;Wyss, JM;Clinton, SM;Kerman, IA; | Neonatal maternal separation stress elicits lasting DNA methylation changes in the hippocampus of stress-reactive Wistar Kyoto rats | Eur. J. Neurosci. | 27643783 | ; Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) for next‐generation sequencing using NEBNext reagents (New England Biolabs, Ipswich, MA) according to manufacturer's recommendations | Full Paper |
2016 | Beck, BH;Fuller, SA;Li, C;Green, BW;Zhao, H;Rawles, SD;Webster, CD;Peatman, E; | Hepatic transcriptomic and metabolic responses of hybrid striped bass (Morone saxatilis×Morone chrysops) to acute and chronic hypoxic insult | Comp. Biochem. Physiol. Part D Genomics Proteomics | 26851735 | treatment) were used for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25 | Full Paper |
2016 | Póvoa, TF;Oliveira, ER;Basílio-de-Oliveira, CA;Nuovo, GJ;Chagas, VL;Salomão, NG;de Barcelos Alves, AM;Mota, EM;Paes, MV; | Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells | PLoS ONE | 28006034 | Janeiro, Brazil. ⨯. Gerard J. Nuovo, Affiliations Ohio State University Comprehensive Cancer Center, Columbus, Ohio, United States of America, Phylogeny Inc, Powell, Ohio, United States of America. ⨯. Vera LA Chagas, Affiliation | Full Paper |
2016 | Stavropoulos, SW;Chen, JX;Sing, RF;Elmasri, F;Silver, MJ;Powell, A;Lynch, FC;Abdel Aal, AK;Lansky, A;Muhs, BE;, ; | Analysis of the Final DENALI Trial Data: A Prospective, Multicenter Study of the Denali Inferior Vena Cava Filter | J Vasc Interv Radiol | 27569678 | Full Paper | |
2016 | Gardet, A;Chou, WC;Reynolds, TL;Velez, DB;Fu, K;Czerkowicz, JM;Bajko, J;Ranger, AM;Allaire, N;Kerns, HM;Ryan, S;Legault, HM;Dunstan, RW;Lafyatis, R;Lukashev, M;Viney, JL;Browning, JL;Rabah, D; | Pristane-Accelerated Autoimmune Disease in (SWR X NZB) F1 Mice Leads to Prominent Tubulointerstitial Inflammation and Human Lupus Nephritis-Like Fibrosis | PLoS ONE | 27760209 | AZ, USA). Needle biopsies from 5 patients with LN (Class II, 1 patient; Class III, 1 patient; Class IV, 1 patient; Class IV/V, 2 patients) were obtained from Folio Biosciences (Powell, OH) and stained using the same methods. For | Full Paper |
2016 | Shearstone, JR;Golonzhka, O;Chonkar, A;Tamang, D;van Duzer, JH;Jones, SS;Jarpe, MB; | Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2 | PLoS ONE | 27073918 | CS2 differentiation media is same as CS2 expansion media, except 3 U/mL EPO. Peripheral blood from patients homozygous for the sickle cell mutation (Conversant Bio) were collected in heparinized tubes and shipped overnight on cool packs | Full Paper |
2016 | Shimizu, T;Seto, T;Hirai, F;Takenoyama, M;Nosaki, K;Tsurutani, J;Kaneda, H;Iwasa, T;Kawakami, H;Noguchi, K;Shimamoto, T;Nakagawa, K; | Phase 1 study of pembrolizumab (MK-3475; anti-PD-1 monoclonal antibody) in Japanese patients with advanced solid tumors | Invest New Drugs | 27000274 | on the confirmation test. PD-L1 expression. PD-L1 expression was measured using immunohistochemistry performed on formalin-fixed, paraffin-embedded tissue sections at QualTek Clinical Laboratories. PD-L1 positivity was ;or postdose sample was po | Full Paper |
2016 | Meaburn, KJ;Agunloye, O;Devine, M;Leshner, M;Roloff, GW;True, LD;Misteli, T; | Tissue-of-origin-specific gene repositioning in breast and prostate cancer | Histochem. Cell Biol. | 26791532 | Tissue sections and tissue microarrays (TMAs) were purchased from US Biomax Inc, Imgenex Corporation, Folio Bioscience and Biochain Institute or were acquired from the University of Washington under the guidelines and approval of the Institutional Re | Full Paper |
2016 | Quadrini, KJ;Hegelund, AC;Cortes, KE;Xue, C;Kennelly, SM;Ji, H;Högerkorp, CM;Mc Closkey, TW; | Validation of a flow cytometry-based assay to assess C5aR receptor occupancy on neutrophils and monocytes for use in drug development | Cytometry B Clin Cytom | 26084468 | a specific Disease Activity Score 28‐joint counts (DAS28) criteria of C‐reactive protein (CRP) ≥ 3.2 and ≥ 2 swollen joints 10, 11 was drawn into K 2 ‐EDTA phlebotomy tubes and stored at 2-8°C. RA patient samples were obtained from Conversant Biologi | Full Paper |
2016 | Gebauer, A;Schmidt, S;Hoffmann, W; | Status and perspective of lab-on-a-chip systems for common diseases: a systematic review from 2003 to 2013 | Per Med | 29749869 | Diagnosis, monitoring Laboratory Patients with advanced-stage breast cancer 73/73 patients 5/5 negative control Conversant Bio (USA), bio options (USA) ? 61 Prospective experimental study Diagnosis, monitoring ? Patients with metastatic lung (n = 55) | Full Paper |
2016 | Shelton, D;Quinlan, A; | Rapid, Sensitive Cancer Biomarker Screening in cfDNA | Genetic Engineering & Biotechnology News | Untested plasma samples from patients who had previously been classified as KRAS mutation positive or negative by FFPE genotyping were purchased from Conversant Bio and ProMedDx. Figure 3. Multiplex detection of KRAS mutations in patient cell-free DN | Full Paper | |
2016 | Justice, JL;Weese, DA;Santos, SR; | Phylogenetic utility, and variability in structure and content, of complete mitochondrial genomes among genetic lineages of the Hawaiian anchialine shrimp Halocaridina rubra Holthuis 1963 (Atyidae:Decapoda) | Mitochondrial DNA A DNA Mapp Seq Anal | 26061341 | The Atyidae are caridean shrimp possessing hair-like setae on their claws and are important contributors to ecological services in tropical and temperate fresh and brackish water ecosystems. Complete mitochondrial genomes have only been reported from | Full Paper |
2016 | Li, S;Garrett-Bakelman, FE;Chung, SS;Sanders, MA;Hricik, T;Rapaport, F;Patel, J;Dillon, R;Vijay, P;Brown, AL;Perl, AE;Cannon, J;Bullinger, L;Luger, S;Becker, M;Lewis, ID;To, LB;Delwel, R;Löwenberg, B;Döhner, H;Döhner, K;Guzman, ML;Hassane, DC;Roboz, GJ;Grimwade, D;Valk, PJ;D'Andrea, RJ;Carroll, M;Park, CY;Neuberg, D;Levine, R;Melnick, AM;Mason, CE; | Distinct evolution and dynamics of epigenetic and genetic heterogeneity in acute myeloid leukemia | Nature medicine | 27322744 | rough AML_101, DNA and RNA were extracted from mononuclear cell layers using standard techniques. Normal bone marrow controls (NBM: CD34+ mononuclear cells; 7 males and 7 females) were purchased from AllCells (Alameda, CA, USA; n = 5) or isolated usi | Full Paper |
2016 | Dever, DP;Bak, RO;Reinisch, A;Camarena, J;Washington, G;Nicolas, CE;Pavel-Dinu, M;Saxena, N;Wilkens, AB;Mantri, S;Uchida, N;Hendel, A;Narla, A;Majeti, R;Weinberg, KI;Porteus, MH; | CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cells | Nature | 27820943 | blood were purchased from AllCells and thawed according to manufacturer's instructions. CD34 + HSPCs from cord blood were either purchased frozen from AllCells or acquired from Save | Full Paper |
2016 | Briney, B;Sok, D;Jardine, JG;Kulp, DW;Skog, P;Menis, S;Jacak, R;Kalyuzhniy, O;de Val, N;Sesterhenn, F;Le, KM;Ramos, A;Jones, M;Saye-Francisco, KL;Blane, TR;Spencer, S;Georgeson, E;Hu, X;Ozorowski, G;Adachi, Y;Kubitz, M;Sarkar, A;Wilson, IA;Ward, AB;Nemazee, D;Burton, DR;Schief, WR; | Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies | Cell | 27610570 | pathogen free environment. Healthy, HIV-Negative Human PBMCs Leukopaks from two heathy, HIV-negative individuals (a 28 year-old female and a 33 year-old male) were obtained from a commercial vendor (AllCells) using a protocol approved by the Institu | Full Paper |
2016 | Corces, MR;Buenrostro, JD;Wu, B;Greenside, PG;Chan, SM;Koenig, JL;Snyder, MP;Pritchard, JK;Kundaje, A;Greenleaf, WJ;Majeti, R;Chang, HY; | Lineage-specific and single-cell chromatin accessibility charts human hematopoiesis and leukemia evolution | Nature genetics | 27526324 | Normal donor human bone marrow and peripheral blood cells were obtained fresh from AllCells or the Stanford Blood Center. All normal blood cell populations were sorted fresh. Save | Full Paper |
2016 | Kim, JH;Hu, Y;Yongqing, T;Kim, J;Hughes, VA;Le Nours, J;Marquez, EA;Purcell, AW;Wan, Q;Sugita, M;Rossjohn, J;Winau, F; | CD1a on Langerhans cells controls inflammatory skin disease | Nature immunology | 27548435 | lysis by ELISA. K562 cells were routinely tested for mycoplasma contamination, and CD1a expression of transfectants was confirmed before use by flow cytometry. PBMCs were obtained from ConversantBio, ALLCELLS, and the Kraft Family Blood Donor Center | Full Paper |
2016 | Locci, M;Wu, JE;Arumemi, F;Mikulski, Z;Dahlberg, C;Miller, AT;Crotty, S; | Activin A programs the differentiation of human TFH cells | Nature immunology | 27376469 | Leukapheresis sample from a healthy donor was obtained from AllCells, Inc., after a prescreening of multiple donors. Fresh whole blood samples from healthy donors were obtained Save | Full Paper |
2016 | Wang, X;Haylock, D;Hu, CS;Kowalczyk, W;Jiang, T;Qiu, J;Mosoyan, G;He, W;Marshall, N;Mascarenhas, J;Tarasova, A;Brody, J;Winkler, D;Hoffman, R; | A thrombopoietin receptor antagonist is capable of depleting myelofibrosis hematopoietic stem and progenitor cells | Blood | 27114459 | Flow Cytometer (Becton Dickinson, Franklin Lakes, NJ) were used in each experiment. BM CD34+ cells from normal individuals were purchased from AllCells (Alameda, CA). The JAK2, CALR, and MPL mutational status1,22-24 of each patient is shown in Table | Full Paper |
2016 | Zhang, Y;Quarmyne, M;Himburg, H;Yan, X;McBride, W;Jung, M;Chute, J; | A Small Molecule Inhibitor of Protein Tyrosine Phosphatase-Sigma (PTPσ) Promotes Hematopoietic Stem Cell (HSC) Regeneration | Blood | Our laboratory previously discovered that pleiotrophin, a paracrine growth factor secreted by the BM perivascular and endothelial cells, promoted HSC self-renewal and regeneration Save | Full Paper | |
2016 | Iancu-Rubin, C;Fong, H;Mosoyan, G;Patel, A;Sabado, R;Marcia, M;Moshier, E;Ozbek, U;Keyzner, A;Bhardwaj, N;McNiece, I;Tong, J;Hoffman, R; | Preclinical Development of a Cord Blood (CB)-Derived Hematopoietic Stem Cell (HSC) Product for Allogeneic Transplantation in Patients with Hematological Malignancies | Blood | Division of Hematology and Medical Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY++AllCells, LLC, Alameda, CA++Division of Hematology and Medical Oncology, The Tisch Cancer Institute, Icahn School of Medic | Full Paper | |
2016 | Hoff, F;Hu, C;Qiu, Y;Yoo, S;Qutub, A;Horton, T;Kornblau, S; | Recurrent Patterns of Protein Expression Signatures in Pediatric Acute Lymphoblastic Leukemia: Recognition and Therapeutic Guidance | Blood | reverse-phase protein arrays (RPPA) methodology was performed on 73 samples from pediatric patients with ALL, 10 cryopreserved CD34 + normal bone marrow samples (AllCells) Save | Full Paper | |
2016 | Yoshioka, S;Miura, Y;Iwasa, M;Fujishiro, A;Sugino, N;Fujii, S;Nakagawa, Y;Sato, A;Yokota, A;Hirai, H;Ichinohe, T;Takaori, A;Maekawa, T; | Late Adherent Subpopulation in Umbilical Cord Blood Has the Same Characteristics and Hematopoiesis-Supporting Capacity As Mesenchymal Stromal/Stem Cells | Blood | Mesenchymal stromal/stem cells (MSCs) are a major source of cell for cell therapy. MSCs derived from bone marrow (BMMSCs) have been mostly used in clinical applications. BMMSCs can be easily isolated as a cell population that adheres to plastic cultu | Full Paper | |
2016 | Merkel, P;Giclas, H;Gibula, A;Andersen, G;Knight, V;Lin, J;Castro, G;Green, J;Stassinopoulos, A; | Reinvestigation of the T-Cell Inactivation Achieved for the Prevention of TA GV HD By 2500 Gy of Gamma Irradiation, a Comparison with Pathogen Reduction | Blood | Background: Transfusion Associated Graft vs. Host Disease (TA GVHD) results in high morbidity and mortality, caused by contaminating T-cells present in transfused blood products. Gamma irradiation (GI) of platelet components (PC) with a dose of 2500 | Full Paper | |
2016 | Kanter, J;Walters, M;Hsieh, M;Krishnamurti, L;Kwiatkowski, J;Kamble, R;von Kalle, C;Kuypers, F;Cavazzana, M;Leboulch, P;Joseney-Antoine, M;Asmal, M;Thompson, A;Tisdale, J; | Interim Results from a Phase 1/2 Clinical Study of Lentiglobin Gene Therapy for Severe Sickle Cell Disease | Blood | β-globin gene transfer into hematopoietic stem cells (HSCs) has the potential to reduce or eliminate the symptoms and long-term complications of severe sickle cell disease (SCD). LentiGlobin Drug Product (DP) is a gene therapy product containing auto | Full Paper | |
2016 | Thompson, A;Kwiatkowski, J;Rasko, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;von Kalle, C;Kletzel, M;Leboulch, P;Vichinsky, E;Petrusich, A;Asmal, M;Walters, M; | Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia: Update from the Northstar Hgb-204 Phase 1/2 Clinical Study | Blood | BACKGROUND Allogeneic hematopoietic stem cell (HSC) transplant is potentially curative for patients with β-thalassemia major or, as more broadly defined, transfusion dependent β-thalassemia (TDT). However, HSC transplant is generally r | Full Paper | |
2016 | Crews, LA;Balaian, L;Delos Santos, NP;Leu, HS;Court, AC;Lazzari, E;Sadarangani, A;Zipeto, MA;La Clair, JJ;Villa, R;Kulidjian, A;Storb, R;Morris, SR;Ball, ED;Burkart, MD;Jamieson, CHM; | RNA Splicing Modulation Selectively Impairs Leukemia Stem Cell Maintenance in Secondary Human AML | Cell stem cell | 27570067 | Age-related human hematopoietic stem cell (HSC) exhaustion and myeloid-lineage skewing promote oncogenic transformation of hematopoietic progenitor cells into therapy-resistant Save | Full Paper |
2016 | Song, C;Zhang, Y;Li, C;Chen, G;Kang, X;Wang, Q; | Enhanced Nanodrug Delivery to Solid Tumors Based on a Tumor Vasculature-Targeted Strategy | Advanced Functional Materials | Cell Culture: Human malignantglioma U87-MG cells were obtained from The Cell Bank of Type Culture Collection of Chinese Academy of Sciences and HUVECs were purchased from Allcells (China). U87-MG cells were cultured in low-glucose Dulbecco's modified | Full Paper | |
2016 | Sherbenou, DW;Aftab, BT;Su, Y;Behrens, CR;Wiita, A;Logan, AC;Acosta-Alvear, D;Hann, BC;Walter, P;Shuman, MA;Wu, X;Atkinson, JP;Wolf, JL;Martin, TG;Liu, B; | Antibody-drug conjugate targeting CD46 eliminates multiple myeloma cells | The Journal of clinical investigation | 27841764 | bead columns (Stem Cell Technologies). Primary cells were cultured in RPMI1640 media with 100 U/ml penicillin, 100 μg/ml streptomycin, 10% FBS, and 2 ng/ml IL-6. BM and PB controls from normal donors were purchased from AllCells | Full Paper |
2016 | Jiang, X;Hu, C;Arnovitz, S;Bugno, J;Yu, M;Zuo, Z;Chen, P;Huang, H;Ulrich, B;Gurbuxani, S;Weng, H;Strong, J;Wang, Y;Li, Y;Salat, J;Li, S;Elkahloun, AG;Yang, Y;Neilly, MB;Larson, RA;Le Beau, MM;Herold, T;Bohlander, SK;Liu, PP;Zhang, J;Li, Z;He, C;Jin, J;Hong, S;Chen, J; | miR-22 has a potent anti-tumour role with therapeutic potential in acute myeloid leukaemia | Nature communications | 27116251 | (Axis-Shield, Oslo, Norway) according to the manufacturer’s manual. Human normal hematopoietic control cell samples The MNC normal control samples were isolated from normal BM cells purchased from AllCells, LLC (Emeryville, CA) by use of NycoPrep 1. | Full Paper |
2016 | van Overbeek, M;Capurso, D;Carter, MM;Thompson, MS;Frias, E;Russ, C;Reece-Hoyes, JS;Nye, C;Gradia, S;Vidal, B;Zheng, J;Hoffman, GR;Fuller, CK;May, AP; | DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks | Molecular cell | 27499295 | Mobilized peripheral blood HSCs (AllCells # mPB018F) were plated at 1.5 × 105/ml in DMEM/F12 Glutamax supplemented with 100× MNEAA solution, 1,000× 2-mercaptoethanol, 100× StemSpan CC100, 1,000× TPO, and 10,000× GM-CSF | Full Paper |
2016 | Wang, J;DeClercq, JJ;Hayward, SB;Li, PW;Shivak, DA;Gregory, PD;Lee, G;Holmes, MC; | Highly efficient homology-driven genome editing in human T cells by combining zinc-finger nuclease mRNA and AAV6 donor delivery | Nucleic acids research | 26527725 | Fresh purified CD8 + and CD4 + T cells were purchased from AllCells.com and maintained in X-VIVO 15 (Lonza, Basel, Switzerland) supplemented with 10% FBS, 2 mM l-glutamine, 1 Save | Full Paper |
2016 | Beard, RS;Yang, X;Meegan, JE;Overstreet, JW;Yang, CG;Elliott, JA;Reynolds, JJ;Cha, BJ;Pivetti, CD;Mitchell, DA;Wu, MH;Deschenes, RJ;Yuan, SY; | Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndrome | Nature communications | 27653213 | Full Paper | |
2016 | Zhao, Y;Wang, A;Zou, Y;Su, N;Loscalzo, J;Yang, Y; | In vivo monitoring of cellular energy metabolism using SoNar, a highly responsive sensor for NAD(+)/NADH redox state | Nature protocols | 27362337 | Cells of interest: we have used the following: NCI-H1299 non-small-cell lung cancer (ATCC, cat. no. CRL-5803), primary mouse hepatocytes (freshly isolated), primary mouse renal tubular epithelial cells (AllCells, cat. no. MRTEC-001F), primary mouse a | Full Paper |
2016 | Li, L;Qi, X;Sun, W;Abdel-Azim, H;Lou, S;Zhu, H;Prasadarao, NV;Zhou, A;Shimada, H;Shudo, K;Kim, YM;Khazal, S;He, Q;Warburton, D;Wu, L; | Am80-GCSF synergizes myeloid expansion and differentiation to generate functional neutrophils that reduce neutropenia-associated infection and mortality | EMBO molecular medicine | 27737899 | opoietin. Primary cells were split every 3 days with equal portions of fresh MM‐HSCCM. Normal primary human hematopoietic precursors and cell culture Human umbilical cord blood CD34+ precursors (AllCells, Emeryville, CA) were expanded, as described | Full Paper |
2016 | Sun, H;Lin, DC;Cao, Q;Guo, X;Marijon, H;Zhao, Z;Gery, S;Xu, L;Yang, H;Pang, B;Lee, VK;Lim, HJ;Doan, N;Said, JW;Chu, P;Mayakonda, A;Thomas, T;Forscher, C;Baloglu, E;Shacham, S;Rajalingam, R;Koeffler, HP; | CRM1 Inhibition Promotes Cytotoxicity in Ewing Sarcoma Cells by Repressing EWS-FLI1-Dependent IGF-1 Signaling | Cancer research | 26956669 | IGFBP3 siRNA lentiviral plasmid (i010368) and EWS-FLI siRNAs (ABM Inc.); Matrigel anti-rabbit IgG and anti-mouse conjugated HRP antibodies (BD Biosciences). siRNA pools targeting DDIT3 and p53 were purchased from Dharmacon. Bone marrow cells from nor | Full Paper |
2016 | Prabhala, RH;Fulciniti, M;Pelluru, D;Rashid, N;Nigroiu, A;Nanjappa, P;Pai, C;Lee, S;Prabhala, NS;Bandi, RL;Smith, R;Lazo-Kallanian, SB;Valet, S;Raje, N;Gold, JS;Richardson, PG;Daley, JF;Anderson, KC;Ettenberg, SA;Di Padova, F;Munshi, NC; | Targeting IL-17A in multiple myeloma: a potential novel therapeutic approach in myeloma | Leukemia | 26293646 | med consent in accordance with the Declaration of Helsinki and approved by the institutional review board (IRB) from Dana-Farber Cancer Institute. Healthy donor bone marrow samples were obtained from AllCells (Emeryville, CA). Myeloma cell-prolifera | Full Paper |
2016 | Su, LF;Del Alcazar, D;Stelekati, E;Wherry, EJ;Davis, MM; | Antigen exposure shapes the ratio between antigen-specific Tregs and conventional T cells in human peripheral blood | Proceedings of the National Academy of Sciences of the United States of America | 27681619 | Human HLA-DR4 umbilical cord blood was purchased from AllCells and from the New York Human HLA-DR4 umbilical cord bloods were purchased from AllCells and from the New Save | Full Paper |
2016 | Ye, L;Wang, J;Tan, Y;Beyer, AI;Xie, F;Muench, MO;Kan, YW; | Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and β-thalassemia | Proceedings of the National Academy of Sciences of the United States of America | 27601644 | according to the manufacturer’s instruction. Peripheral blood mobilized CD34+ HSPCs and bone marrow CD34+ HSPCs were purchased from AllCells and cultured in StemSpan SFEM (Stemcell Technologies) supplemented with human cytokines, 100 ng/mL SCF, 100 n | Full Paper |
2016 | Tarafder, S;Koch, A;Jun, Y;Chou, C;Awadallah, MR;Lee, CH; | Micro-precise spatiotemporal delivery system embedded in 3D printing for complex tissue regeneration | Biofabrication | 27108484 | MSCs were isolated from human bone marrow (AllCells, Alameda, CA) following our established protocol [5, 13]. P3-5 MSCs at a density of 1 × 10 6 cells ml −1 were seeded into the Save | Full Paper |
2016 | Horiguchi, H;Kobune, M;Kikuchi, S;Yoshida, M;Murata, M;Murase, K;Iyama, S;Takada, K;Sato, T;Ono, K;Hashimoto, A;Tatekoshi, A;Kamihara, Y;Kawano, Y;Miyanishi, K;Sawada, N;Kato, J; | Extracellular vesicle miR-7977 is involved in hematopoietic dysfunction of mesenchymal stromal cells via poly(rC) binding protein 1 reduction in myeloid neoplasms | Haematologica | 26802051 | MSCs from healthy volunteers (HVs) were purchased from AllCells, LLC (Toronto, Canada) (HV-derived MSCs #1, #2, #3 and #4). Human primary MSCs and human BM CD34+ cells derived from lymphoma patients stage I/II without bone marrow localization (contro | Full Paper |
2016 | Li, D;Xie, K;Zhang, L;Yao, X;Li, H;Xu, Q;Wang, X;Jiang, J;Fang, J; | Dual blockade of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) exhibits potent anti-angiogenic effects | Cancer letters | 27130666 | Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China) and maintained in a complete HUVEC medium with 10% FBS (Invitrogen) and 10% Save | Full Paper |
2016 | de Jong, RN;Beurskens, FJ;Verploegen, S;Strumane, K;van Kampen, MD;Voorhorst, M;Horstman, W;Engelberts, PJ;Oostindie, SC;Wang, G;Heck, AJ;Schuurman, J;Parren, PW; | A Novel Platform for the Potentiation of Therapeutic Antibodies Based on Antigen-Dependent Formation of IgG Hexamers at the Cell Surface | PLoS biology | 26736041 | nuously cultured systems to ensure authenticity of the cell lines. Pooled normal human serum (NHS) AB was obtained from Sanquin (The Netherlands). Primary B cells from CLL patients were obtained from AllCells (Alameda, CA). Animals Eight- to 11-wk-o | Full Paper |
2016 | Carmi, Y;Prestwood, TR;Spitzer, MH;Linde, IL;Chabon, J;Reticker-Flynn, NE;Bhattacharya, N;Zhang, H;Zhang, X;Basto, PA;Burt, BM;Alonso, MN;Engleman, EG; | Akt and SHP-1 are DC-intrinsic checkpoints for tumor immunity | JCI insight | 27812544 | For some experiments, BM cells and peripheral blood from the same donor were purchased from AllCells. The cells were applied to a Ficoll gradient (BD), and CD34 + cells were Save | Full Paper |
2016 | Legemate, K;Tarafder, S;Jun, Y;Lee, CH; | Engineering Human TMJ Discs with Protein-Releasing 3D-Printed Scaffolds | Journal of dental research | 27053116 | Human MSCs were isolated from commercially available, fresh whole bone marrow samples of anonymous adult donors (AllCells) (age range: 20-25 y) per our previous methods (Lee et al. 2009; Lee, Shah, et al. 2010; Lee et al. 2014). Following our establi | Full Paper |
2016 | Kota, DJ;Prabhakara, KS;van Brummen, AJ;Bedi, S;Xue, H;DiCarlo, B;Cox, CS;Olson, SD; | Propranolol and Mesenchymal Stromal Cells Combine to Treat Traumatic Brain Injury | Stem cells translational medicine | 26586775 | We isolated MSCs from commercially available fresh human bone marrow aspirates of a 34-year-old male (AllCells, Emeryville, CA, http://www.allcells.com) according to the protocols previously established using density centrifugation and plastic adhere | Full Paper |
2016 | Lee, SJ;Levitsky, K;Parlati, F;Bennett, MK;Arastu-Kapur, S;Kellerman, L;Woo, TF;Wong, AF;Papadopoulos, KP;Niesvizky, R;Badros, AZ;Vij, R;Jagannath, S;Siegel, D;Wang, M;Ahmann, GJ;Kirk, CJ; | Clinical activity of carfilzomib correlates with inhibition of multiple proteasome subunits: application of a novel pharmacodynamic assay | British journal of haematology | 27071340 | Human whole blood and peripheral blood mononuclear cell (PBMC)-enriched leukapheresis-derived samples from healthy volunteers were purchased from AllCells (Berkeley, CA, Save | Full Paper |
2016 | Jones, RB;Mueller, S;O'Connor, R;Rimpel, K;Sloan, DD;Karel, D;Wong, HC;Jeng, EK;Thomas, AS;Whitney, JB;Lim, SY;Kovacs, C;Benko, E;Karandish, S;Huang, SH;Buzon, MJ;Lichterfeld, M;Irrinki, A;Murry, JP;Tsai, A;Yu, H;Geleziunas, R;Trocha, A;Ostrowski, MA;Irvine, DJ;Walker, BD; | A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes | PLoS pathogens | 27082643 | the HIV-1 Test, v2.0 kit (Roche Diagnostics). Primary CD4+ T cell HIV latency model Total peripheral blood mononuclear cells (PBMCs) were obtained from healthy HIV-negative donors by leukapheresis (AllCells, Inc, Emeryville, CA). Naive CD4+ T cells | Full Paper |
2016 | Clark, RS;Pellom, ST;Booker, B;Ramesh, A;Zhang, T;Shanker, A;Maguire, M;Juarez, PD;Patricia, MJ;Langston, MA;Lichtveld, MY;Hood, DB; | Validation of research trajectory 1 of an Exposome framework: Exposure to benzo(a)pyrene confers enhanced susceptibility to bacterial infection | Environmental research | 26765097 | Fresh human CD14+ monocytes were obtained from AllCells (Alameda, CA) and were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated FBS (HyClone), 100 U/penicillin, 100 U/mL streptomycin (GIBCO) in the presence of DMSO, B(a)P (1, 5, 10 | Full Paper |
2016 | Zhang, W;Cao, H;Zhang, X;Li, G;Chang, Q;Zhao, J;Qiao, Y;Ding, X;Yang, G;Liu, X;Jiang, X; | A strontium-incorporated nanoporous titanium implant surface for rapid osseointegration | Nanoscale | 26881868 | In this experiment, endothelial cell growth medium (AllCells) was used to collect the secreted growth factors, and then was taken for HUVECS culture for 24 hours to form cellular Save | Full Paper |
2016 | Wu, J;Sun, Y;Block, TJ;Marinkovic, M;Zhang, ZL;Chen, R;Yin, Y;Song, J;Dean, DD;Lu, Z;Chen, XD; | Umbilical cord blood-derived non-hematopoietic stem cells retrieved and expanded on bone marrow-derived extracellular matrix display pluripotent characteristics | Stem cell research & therapy | 27906056 | Freshly isolated human BM-MNCs (containing MSCs), obtained from 20- to 30-year-old donors and purchased from ALLCELLS (Emeryville, CA, USA), were used to prepare BM stromal Save | Full Paper |
2016 | Umemura, N;Ohkoshi, E;Tajima, M;Kikuchi, H;Katayama, T;Sakagami, H; | Hyaluronan induces odontoblastic differentiation of dental pulp stem cells via CD44 | Stem cell research & therapy | 27651223 | s modified Eagle’s medium (DMEM) was purchased from Invitrogen (Carlsbad, CA, USA). Fetal bovine serum (FBS) was purchased from Nichirei Bioscience (Tokyo, Japan). Human DPSCs were obtained from AllCells LLC (Emeryville, CA, USA). Cell cultures were | Full Paper |
2016 | Frasheri, I;Ern, C;Diegritz, C;Hickel, R;Hristov, M;Folwaczny, M; | Full-length amelogenin influences the differentiation of human dental pulp stem cells | Stem cell research & therapy | 26762641 | The human dental pulp stem cells hDPSCs were obtained from AllCells (Alameda, CA, USA). They had been characterized by the company by means of flow cytometry, revealing a Save | Full Paper |
2016 | Chen, W;Wang, S;Adhikari, S;Deng, Z;Wang, L;Chen, L;Ke, M;Yang, P;Tian, R; | Simple and Integrated Spintip-Based Technology Applied for Deep Proteome Profiling | Analytical chemistry | 27062885 | The SHED cell line was purchased from Allcells (Alameda, CA) and cultured in α-modified Eagle's medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/ Save | Full Paper |
2016 | Gonçalves Silva, I;Rüegg, L;Gibbs, BF;Bardelli, M;Fruehwirth, A;Varani, L;Berger, SM;Fasler-Kan, E;Sumbayev, VV; | The immune receptor Tim-3 acts as a trafficker in a Tim-3/galectin-9 autocrine loop in human myeloid leukemia cells | Oncoimmunology | 27622049 | ). All other chemicals purchased were of the highest grade of purity. Primary human AML and CLL cells Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accorda | Full Paper |
2016 | Yang, J;Liu, W;Lv, J;Feng, Y;Ren, X;Zhang, W; | REDV-polyethyleneimine complexes for selectively enhancing gene delivery in endothelial cells | Journal of materials chemistry. B | 32263272 | The pEGFP-ZNF580 plasmid (pZNF580) was preserved by Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force. CREDVW and CRE[V with combining low line][D with combining low line]W peptides were supplie | Full Paper |
2016 | Ma, SD;Xu, X;Jones, R;Delecluse, HJ;Zumwalde, NA;Sharma, A;Gumperz, JE;Kenney, SC; | PD-1/CTLA-4 Blockade Inhibits Epstein-Barr Virus-Induced Lymphoma Growth in a Cord Blood Humanized-Mouse Model | PLoS pathogens | 27186886 | diabetic/severe combined immunodeficient (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson Labs (catalogue 005557). Commercially purchased CD34-depleted human cord blood mononuclear cells (AllCells, LLC., CB117) were mock-infected, or infect | Full Paper |
2016 | Ebina, W; | Combinatorial Pathway Modulation Toward Ex Vivo Maintenance and Propagation of Hematopoietic Stem Cells | Thesis | CD34 enriched human cord blood, mobilized peripheral blood, or human bone marrow (AllCells) were thawed, washed in PBS with 2mM EDTA and 2%FBS, and stained for the following markers: Lineage(CD235a, CD3, CD19, CD20, CD56), CD34, CD38, CD45RA, and CD9 | Full Paper | |
2016 | Yang, T;Yao, Q;Cao, F;Liu, Q;Liu, B;Wang, XH; | Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis | International journal of nanomedicine | 27994464 | nd the human lymphoma cell lines Raji and Daudi were obtained from the American Type Culture Collection (Manassas, VA, USA). Human umbilical vein endothelial cells (HUVEC) were obtained from Shanghai AllCells Biotech Co. Ltd. (Shanghai, People’s Repu | Full Paper |
2016 | Moot, R;Raikar, SS;Fleischer, L;Querrey, M;Tylawsky, DE;Nakahara, H;Doering, CB;Spencer, HT; | Genetic engineering of chimeric antigen receptors using lamprey derived variable lymphocyte receptors | Molecular therapy oncolytics | 27933313 | d using a BD FACS Aria II Cell Sorter (BD Biosciences). Expansion and lentiviral transduction of primary human T cells Commercially available human peripheral blood mononuclear cells were purchased (AllCells, Catalog # PB004F, Alameda, CA). After th | Full Paper |
2016 | Hall, BM;Balan, V;Gleiberman, AS;Strom, E;Krasnov, P;Virtuoso, LP;Rydkina, E;Vujcic, S;Balan, K;Gitlin, I;Leonova, K;Polinsky, A;Chernova, OB;Gudkov, AV; | Aging of mice is associated with p16(Ink4a)- and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cells | Aging | 27391570 | f p16(Ink4a)-positive cells [21,23] and may reflect an exaggerated view on SCs as the sole source of inflammaging. MATERIALS AND METHODS Cell culture Primary human neonatal dermal fibroblasts (NDFs; AllCells, LLC) were pooled equally from three sepa | Full Paper |
2016 | Naka, K;Ishihara, K;Jomen, Y;Jin, CH;Kim, DH;Gu, YK;Jeong, ES;Li, S;Krause, DS;Kim, DW;Bae, E;Takihara, Y;Hirao, A;Oshima, H;Oshima, M;Ooshima, A;Sheen, YY;Kim, SJ;Kim, DK; | Novel oral transforming growth factor-β signaling inhibitor EW-7197 eradicates CML-initiating cells | Cancer science | 26583567 | Full Paper | |
2016 | Rovira Gonzalez, YI;Lynch, PJ;Thompson, EE;Stultz, BG;Hursh, DA; | In vitro cytokine licensing induces persistent permissive chromatin at the Indoleamine 2,3-dioxygenase promoter | Cytotherapy | 27421739 | Bone marrow-derived MSCs were obtained from AllCells (Alameda, CA), Lonza (Allendale, NJ), and RoosterBio (Frederick, MD) at passage one (Supplemental Table 1). MSCs in this study were previously expanded, characterized, and cryopreserved to generate | Full Paper |
2016 | Sutton, KS;Dasgupta, A;McCarty, D;Doering, CB;Spencer, HT; | Bioengineering and serum free expansion of blood-derived γδ T cells | Cytotherapy | 27260209 | Commercially available PBMCs were obtained via Leuko Pak apheresis from 11 healthy donors (AllCells). Nine frozen and two fresh PBMCs were used. Frozen PBMCs were rapidly Save | Full Paper |
2016 | Stultz, BG;McGinnis, K;Thompson, EE;Lo Surdo, JL;Bauer, SR;Hursh, DA; | Chromosomal stability of mesenchymal stromal cells during in vitro culture | Cytotherapy | 26780865 | Both AllCells and Lonza use standard protocols for isolation of MSCs from bone marrow and culture cells in Mesencult (Stem Cell Technologies) or MSCGM (Lonza), respectively. Save | Full Paper |
2016 | Imamichi, Y;Yuhki, KI;Orisaka, M;Kitano, T;Mukai, K;Ushikubi, F;Taniguchi, T;Umezawa, A;Miyamoto, K;Yazawa, T; | 11-Ketotestosterone Is a Major Androgen Produced in Human Gonads | The Journal of clinical endocrinology and metabolism | 27428878 | Plasma was separated by centrifugation at 1000g for 5 minutes. Other plasma samples were purchased from AllCells and ProMedDX. All plasma samples were collected under IRB-approved collection protocols and subject informed consent. The donors were 10 | Full Paper |
2016 | Tsiang, M;Jones, GS;Goldsmith, J;Mulato, A;Hansen, D;Kan, E;Tsai, L;Bam, RA;Stepan, G;Stray, KM;Niedziela-Majka, A;Yant, SR;Yu, H;Kukolj, G;Cihlar, T;Lazerwith, SE;White, KL;Jin, H; | Antiviral Activity of Bictegravir (GS-9883), a Novel Potent HIV-1 Integrase Strand Transfer Inhibitor with an Improved Resistance Profile | Antimicrobial agents and chemotherapy | 27645238 | the pseudotyped particles from SODk1 2G cells has been previously described (29). Freshly isolated human peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers (supplied by AllCells, Inc., Alameda, CA). Blood donors were ne | Full Paper |
2016 | Feng, JY;Xu, Y;Barauskas, O;Perry, JK;Ahmadyar, S;Stepan, G;Yu, H;Babusis, D;Park, Y;McCutcheon, K;Perron, M;Schultz, BE;Sakowicz, R;Ray, AS; | Role of Mitochondrial RNA Polymerase in the Toxicity of Nucleotide Inhibitors of Hepatitis C Virus | Antimicrobial agents and chemotherapy | 26596942 | Normal human primary bone marrow (BM) light-density cells were from three different lots obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD). Primary rat neonatal Save | Full Paper |
2016 | Yoon, J;Wang, H;Kim, YC;Yoshimoto, M;Abbasi, S;Morse Iii, HC; | Plasma cell alloantigen ENPP1 is expressed by a subset of human B cells with potential regulatory functions | Immunology and cell biology | 27029896 | 1 mm sodium pyruvate and HEPES) immediately before each experiment. Cord blood cells were purchased from AllCells LLC (Emeryville, CA. USA). Tonsillar tissues were kindly provided by Dr Leonid B Margolis (National Institute of Child Health and Human | Full Paper |
2016 | Marklein, RA;Lo Surdo, JL;Bellayr, IH;Godil, SA;Puri, RK;Bauer, SR; | High Content Imaging of Early Morphological Signatures Predicts Long Term Mineralization Capacity of Human Mesenchymal Stem Cells upon Osteogenic Induction | Stem cells (Dayton, Ohio) | 26865267 | Human bone marrow-derived stem cells were obtained from eight different donors purchased from either Lonza (Walkersville, MD, www.lonza.com) (167696, 110877, 8F3560, 127756) or All Cells (Emeryville, CA, www.allcells.com) (PCBM1632, PCBM1641, PCBM165 | Full Paper |
2016 | Rosa, V;Xie, H;Dubey, N;Madanagopal, TT;Rajan, SS;Morin, JL;Islam, I;Castro Neto, AH; | Graphene oxide-based substrate: physical and surface characterization, cytocompatibility and differentiation potential of dental pulp stem cells | Dental materials : official publication of the Academy of Dental Materials | 27283997 | The use of human cells in this study was approved by NUS Institutional Review Board (Approval Number: NUS 2094). Human dental pulp stem cells from single donor (DPF003, Allcells, USA) were sorted by fluorescence-activated cell sorting analysis (FACS, | Full Paper |
2016 | Wang, J;Zhang, Y;Zhu, Q;Liu, Y;Cheng, H;Zhang, Y;Li, T; | Emodin protects mice against radiation-induced mortality and intestinal injury via inhibition of apoptosis and modulation of p53 | Environmental toxicology and pharmacology | 27525562 | The aim of this study was to explore the protective effect of emodin, a plant-derived anthraquinone, against gamma radiation-induced mortality and intestinal injury in mice, and to Save | Full Paper |
2016 | Walters, MC;De Castro, LM;Sullivan, KM;Krishnamurti, L;Kamani, N;Bredeson, C;Neuberg, D;Hassell, KL;Farnia, S;Campbell, A;Petersdorf, E; | Indications and Results of HLA-Identical Sibling Hematopoietic Cell Transplantation for Sickle Cell Disease | Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation | 26500093 | Financial disclosure: MCW is medical director for the ViaCord Processing Laboratory and AllCells, Inc. He is a consultant for bluebird bio, Inc, Sangamo Biosciences, Inc and Bayer. Save | Full Paper |
2016 | Hsu, H;Boudova, S;Mvula, G;Divala, TH;Mungwira, RG;Harman, C;Laufer, MK;Pauza, CD;Cairo, C; | Prolonged PD1 Expression on Neonatal Vδ2 Lymphocytes Dampens Proinflammatory Responses: Role of Epigenetic Regulation | Journal of immunology (Baltimore, Md. : 1950) | 27474072 | commercial specimens obtained from AllCells. Although we cannot exclude that we introduced a bias in our study, this seems unlikely considering that the AllCells specimens were not Save | Full Paper |
2016 | Chen, LT;Hu, MM;Xu, ZS;Liu, Y;Shu, HB; | MSX1 Modulates RLR-Mediated Innate Antiviral Signaling by Facilitating Assembly of TBK1-Associated Complexes | Journal of immunology (Baltimore, Md. : 1950) | 27194789 | Generation and transfection of human primary macrophages were performed, as previously described (36). The human CD14+ monocytes were purchased from ALLCELLS. For generation of macrophages, the CD14+ monocytes were treated with recombinant M-CSF (50 | Full Paper |
2016 | Gao, W;Liu, H;Yuan, J;Wu, C;Huang, D;Ma, Y;Zhu, J;Ma, L;Guo, J;Shi, H;Zou, Y;Ge, J; | Exosomes derived from mature dendritic cells increase endothelial inflammation and atherosclerosis via membrane TNF-α mediated NF-κB pathway | Journal of cellular and molecular medicine | 27515767 | P (Ribobio, Guangzhou, China) was used. SiRNA TNF‐α was also purchased from Ribobio and the transfected concentration was 50 nM. HUVECs culture and intervention Primary HUVECs were purchased from AllCells (Shanghai, China) and cultured with a specia | Full Paper |
2016 | Zhou, Y;Li, XH;Zhang, CC;Wang, MJ;Xue, WL;Wu, DD;Ma, FF;Li, WW;Tao, BB;Zhu, YC; | Hydrogen sulfide promotes angiogenesis by downregulating miR-640 via the VEGFR2/mTOR pathway | American journal of physiology. Cell physiology | 26879375 | Pooled human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in endothelial basal medium (EBM; Allcells) supplemented with Save | Full Paper |
2016 | Lowery, JW;Ankrum, JA;Kokabu, S;Liu, R; | Targeted Strategies to Modulate Stem-Cell-Relevant Pathways | Stem cells international | 27703484 | + CB progenitors (also referred to as "starting CB progenitors") from pooled donors were purchased from AllCells (Emeryville, CA) and cultured in the hematopoietic growth medium ( Save | Full Paper |
2016 | Nagaria, PK;Robert, C;Park, TS;Huo, JS;Zambidis, ET;Rassool, FV; | High-Fidelity Reprogrammed Human IPSCs Have a High Efficacy of DNA Repair and Resemble hESCs in Their MYC Transcriptional Signature | Stem cells international | 27688775 | + CB progenitors (also referred to as "starting CB progenitors") from pooled donors were purchased from AllCells (Emeryville, CA) and cultured in the hematopoietic growth medium ( Save | Full Paper |
2016 | Shibata, T;Ohto, U;Nomura, S;Kibata, K;Motoi, Y;Zhang, Y;Murakami, Y;Fukui, R;Ishimoto, T;Sano, S;Ito, T;Shimizu, T;Miyake, K; | Guanosine and its modified derivatives are endogenous ligands for TLR7 | International immunology | 26489884 | Human macrophages were induced from human peripheral blood mononuclear cells (hPBMCs), purchased from Allcells (Alameda, CA, USA) and Cellular Technology Limited (Shaker Height, OH, USA). hPBMCs were plated in 10-cm cell-culture dishes with a density | Full Paper |
2016 | Pineda, G;Lennon, KM;Delos Santos, NP;Lambert-Fliszar, F;Riso, GL;Lazzari, E;Marra, MA;Morris, S;Sakaue-Sawano, A;Miyawaki, A;Jamieson, CH; | Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche | Scientific reports | 27041210 | amples were obtained from patient peripheral blood that was collected with informed consent for research use in accordance with Institutional Review Board approved protocols at UCSD or purchased from AllCells (Alameda, CA). Normal peripheral blood mo | Full Paper |
2016 | Zou, L;Chen, Q;Quanbeck, Z;Bechtold, JE;Kaufman, DS; | Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells | Scientific reports | 26980556 | Human BM-MSCs were isolated from fresh bone marrow (AllCells) according to the recommended protocol and expanded in MSC culture media, consisting of 10% defined fetal bovine Save | Full Paper |
2016 | Ling, C;Bhukhai, K;Yin, Z;Tan, M;Yoder, MC;Leboulch, P;Payen, E;Srivastava, A; | High-Efficiency Transduction of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for Overcoming Donor-Variation and Implications in Genome Editing | Scientific reports | 27759036 | ary HSPCs derived from bone marrow (BM) as well as from umbilical cord blood (CB). BM-derived CD34+ cells from individual donors (or a mixture from 10 donors) were purchased from a commercial source (AllCells, LLC, Alameda, CA, USA), and were used to | Full Paper |
2016 | Tian, GA;Zhu, CC;Zhang, XX;Zhu, L;Yang, XM;Jiang, SH;Li, RK;Tu, L;Wang, Y;Zhuang, C;He, P;Li, Q;Cao, XY;Cao, H;Zhang, ZG; | CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinib | Scientific reports | 27506146 | HUVECs were obtained from the Fifth People's Hospital of Shanghai and cultured in endothelial cell complete medium containing endothelial cell growth supplement (Allcells; H-004). Save | Full Paper |
2016 | Kim, YC;Cui, J;Luo, J;Xiao, F;Downs, B;Wang, SM; | Exome-based Variant Detection in Core Promoters | Scientific reports | 27464681 | Caucasian male donor were purchased from AllCells Company (AllCells, Alameda, California). The use of the cells for research purpose has AllCells company IRB approval (Biomed Save | Full Paper |
2016 | Chalmers, SA;Doerner, J;Bosanac, T;Khalil, S;Smith, D;Harcken, C;Dimock, J;Der, E;Herlitz, L;Webb, D;Seccareccia, E;Feng, D;Fine, JS;Ramanujam, M;Klein, E;Putterman, C; | Therapeutic Blockade of Immune Complex-Mediated Glomerulonephritis by Highly Selective Inhibition of Bruton's Tyrosine Kinase | Scientific reports | 27192942 | Primary CD19+ cells were purified from human peripheral blood mononuclear cells (AllCells frozen peripheral blood mononuclear cells (AllCells) and negatively selected by magnetic Save | Full Paper |
2016 | Moisseiev, E;Smit-McBride, Z;Oltjen, S;Zhang, P;Zawadzki, RJ;Motta, M;Murphy, CJ;Cary, W;Annett, G;Nolta, JA;Park, SS; | Intravitreal Administration of Human Bone Marrow CD34+ Stem Cells in a Murine Model of Retinal Degeneration | Investigative ophthalmology & visual science | 27537262 | Fresh human BM aspirate was purchased from AllCells (Alameda, CA, USA) and obtained for the scheduled day of use. The BM aspirate for this study was obtained from a 38-year-old Save | Full Paper |
2016 | Hassan, HE;Keita, JA;Narayan, L;Brady, SM;Frederick, R;Carlson, S;C Glass, K;Natesan, S;Buttolph, T;Fandy, TE; | The combination of dimethoxycurcumin with DNA methylation inhibitor enhances gene re-expression of promoter-methylated genes and antagonizes their cytotoxic effect | Epigenetics | 27588609 | Frozen acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) bone marrow mononuclear cells (BMNC) were purchased from Allcells (Alameda, CA). BMNC were obtained from newly diagnosed and untreated patients. The viability of the cells was tes | Full Paper |
2016 | Hu, X;Hu, T;Shen, G;Lian, M;Guan, G;Wang, F;Wang, L; | PCL films of varying porosity influence ICAM-1 expression of HUVECs | Journal of biomedical materials research. Part A | 27345288 | The HUVECs (HUVEC001) provided by Allcells Company (Shanghai, China) were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco), and 1% penicillin-streptomycin (Gibco) at 37°C in a 5% CO2 incubator. Before see | Full Paper |
2016 | Lin, HA;Gupta, MS;Varma, DM;Gilchrist, ML;Nicoll, SB; | Lower crosslinking density enhances functional nucleus pulposus-like matrix elaboration by human mesenchymal stem cells in carboxymethylcellulose hydrogels | Journal of biomedical materials research. Part A | 26256108 | Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density gradient centrifugation as previously described.18 Briefly, bone marrow aspirate was Save | Full Paper |
2016 | Rogers, LJ;Basnakian, AG;Orloff, MS;Ning, B;Yao-Borengasser, A;Raj, V;Kadlubar, S; | 2-amino-1-methyl-6-phenylimidazo(4,5-b) pyridine (PhIP) induces gene expression changes in JAK/STAT and MAPK pathways related to inflammation, diabetes and cancer | Nutrition & metabolism | 27547236 | med by submission to American Type Culture Collection (ATCC) (Manassas, VA) for analysis by short tandem repeat methodology on December 9, 2015. Human Mesenchymal Stem Cells (hMSC) were obtained from AllCells (Emeryville, CA). The hMSCs were expanded | Full Paper |
2016 | Pellegrino, M;Sciambi, A;Yates, JL;Mast, JD;Silver, C;Eastburn, DJ; | RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures | BMC genomics | 27189161 | Anonymous blood samples were initially obtained from a commercial provider (AllCells) that operates with independent IRB approval. Donor anonymity was protected using United Save | Full Paper |
2016 | Holt, A;Wootton, SC;Mulero, JJ;Brzoska, PM;Langit, E;Green, RL; | Developmental validation of the Quantifiler(®) HP and Trio Kits for human DNA quantification in forensic samples | Forensic science international. Genetics | 26774100 | Human female DNA "A3408" was purified from peripheral blood cells obtained from AllCells LLC. (Alameda, CA) using a QIAamp DNA Blood Maxi Kit (Qiagen). Save | Full Paper |
2016 | Pankajakshan, D;Albuquerque, MT;Evans, JD;Kamocka, MM;Gregory, RL;Bottino, MC; | Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function | Journal of endodontics | 27663615 | After 7 days, the specimens were rinsed with 10 mL saline followed by 3 mL EDTA for 3 minutes, and a final abundant rinsing step with saline was performed before cell seeding. Human DPSCs (AllCells, LLC, Alameda, CA) were cultured in alpha-minimum es | Full Paper |
2016 | Nicoletti, AM;Kenny, CH;Khalil, AM;Pan, Q;Ralph, KL;Ritchie, J;Venkataramani, S;Presky, DH;DeWire, SM;Brodeur, SR; | Unexpected Potency Differences between B-Cell-Activating Factor (BAFF) Antagonist Antibodies against Various Forms of BAFF: Trimer, 60-Mer, and Membrane-Bound | The Journal of pharmacology and experimental therapeutics | 27440419 | Negatively selected B cells were obtained from frozen human peripheral blood (AllCells, LLC, Alameda, CA). Sexes of cell donors were as follows: Fig. 2C = 60-mer, two males, one Save | Full Paper |
2016 | Jin, E;Kim, TH;Han, S;Kim, SW; | Amniotic epithelial cells promote wound healing in mice through high epithelialization and engraftment | Journal of tissue engineering and regenerative medicine | 26174407 | Human AMEs were from AllCells (Alameda, CA, USA) and adipose-derived mesenchymal stem cells (ADMs) were from Thermo Scientific Inc. (Rockford, IL, USA). Human keratinocytes were also purchased from ATCC. Cell culture was performed as described previo | Full Paper |
2016 | Yang, H;Sun, J;Li, Y;Duan, WM;Bi, J;Qu, T; | Human umbilical cord-derived mesenchymal stem cells suppress proliferation of PHA-activated lymphocytes in vitro by inducing CD4(+)CD25(high)CD45RA(+) regulatory T cell production and modulating cytokine secretion | Cellular immunology | 26774852 | PBMCs of healthy donors (n = 10) were purchased from AllCells Inc (Emeryville, CA, USA) and StemExpress (Placerville, CA, USA). Co-culture experiments were performed in a 12-well plate (BD, Franklin Lakes, New Jersey, USA). 1 × 105 UC-MSCs were plate | Full Paper |
2016 | Foster, MH;Buckley, ES;Chen, BJ;Hwang, KK;Clark, AG; | Uncommon structural motifs dominate the antigen binding site in human autoantibodies reactive with basement membrane collagen | Molecular immunology | 27450516 | 100 cGy, X-RAD 320, Precision X-ray, North Branford, CT) 9.5-10 week old NSG recipients were intravenously injected with purified human CD34+ HSC (AllCells LLC, Alameda, CA), using 2.5-10×104 CD34+ HSC per mouse (Pearson et al., 2008; Shultz et al., | Full Paper |
2016 | Smith, AJ;Li, Y;Bazin, HG;St-Jean, JR;Larocque, D;Evans, JT;Baldridge, JR; | Evaluation of novel synthetic TLR7/8 agonists as vaccine adjuvants | Vaccine | 27402566 | Human myeloid or plasmacytoid DCs were isolated from PBMCs obtained by leukapheresis from normal donors (AllCells, Berkeley, CA). PBMCs were repeatedly washed in 1× PBS Save | Full Paper |
2016 | Zeng, Y;Liu, J; | Role of glypican-1 in endothelial NOS activation under various steady shear stress magnitudes | Experimental cell research | 27688027 | Human umbilical vein endothelial cells (HUVECs) and all cell culture reagents were purchased from Allcells, China. As previously described [23], HUVECs were cultured in HUVEC medium containing 10% FBS and 10% growth factor supplement containing EGF, | Full Paper |
2016 | Liu, X;Zhang, A;Xiang, J;Lv, Y;Zhang, X; | miR-451 acts as a suppressor of angiogenesis in hepatocellular carcinoma by targeting the IL-6R-STAT3 pathway | Oncology reports | 27461244 | The umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Shanghai, China). HepG2 and HEK293T cells were incubated with Dulbecco's modified Eagle's medium ( Save | Full Paper |
2016 | Peterson, SJ;Vanella, L;Gotlinger, K;Jiang, H;Singh, SP;Sodhi, K;Maher, E;O'Hanlon, K;Shapiro, JI;Abraham, NG; | Oxidized HDL is a potent inducer of adipogenesis and causes activation of the Ang-II and 20-HETE systems in human obese females | Prostaglandins & other lipid mediators | 27179555 | Frozen human bone marrow mononuclear stem cells (MSCs) were purchased from Allcells (Allcells, Emeryville, CA). After thawing, mononuclear cells were resuspended in a minimal essential medium, (Invitrogen, Carlsbad, CA) supplemented with 10% heat ina | Full Paper |
2016 | Ren, X;Li, J;Xu, X;Wang, C;Cheng, Y; | IBI302, a promising candidate for AMD treatment, targeting both the VEGF and complement system with high binding affinity in vitro and effective targeting of the ocular tissue in healthy rhesus monkeys | Experimental eye research | 26919788 | HUVEC basal medium (used for HUVEC proliferation assay) was bought from Allcells (Shanghai, China). Rabbit red blood cells (RRBCs) were purchased from Laboratory animal Save | Full Paper |
2016 | Griffin, DO;Goff, SP; | Restriction of HIV-1-based lentiviral vectors in adult primary marrow-derived and peripheral mobilized human CD34+ hematopoietic stem and progenitor cells occurs prior to viral DNA integration | Retrovirology | 26945863 | ep toward uncovering the molecular mechanism restricting HIV-1 transduction of HPSCs. Methods Source of human cells We obtained primary adult marrow-derived and peripheral mobilized CD34+ cells from AllCells and StemCell Technologies (pre-isolated). | Full Paper |
2016 | Cao, L;Weetall, M;Bombard, J;Qi, H;Arasu, T;Lennox, W;Hedrick, J;Sheedy, J;Risher, N;Brooks, PC;Trifillis, P;Trotta, C;Moon, YC;Babiak, J;Almstead, NG;Colacino, JM;Davis, TW;Peltz, SW; | Discovery of Novel Small Molecule Inhibitors of VEGF Expression in Tumor Cells Using a Cell-Based High Throughput Screening Platform | PloS one | 27992500 | All primary cells were purchased from AllCells, Inc (California, USA) and cultured in RPMI-1640 medium for peripheral blood mononuclear cells (PBMC) or DMEM for adherent cells, Save | Full Paper |
2016 | Pogue, SL;Taura, T;Bi, M;Yun, Y;Sho, A;Mikesell, G;Behrens, C;Sokolovsky, M;Hallak, H;Rosenstock, M;Sanchez, E;Chen, H;Berenson, J;Doyle, A;Nock, S;Wilson, DS; | Targeting Attenuated Interferon-α to Myeloma Cells with a CD38 Antibody Induces Potent Tumor Regression with Reduced Off-Target Activity | PloS one | 27611189 | Bone Marrow cells: Normal and myeloma bone marrow cells were purchased from AllCells, Inc. as frozen vials containing ten million bone marrow mononuclear cells/vial. All donors Save | Full Paper |
2016 | Doss, JF;Jonassaint, JC;Garrett, ME;Ashley-Koch, AE;Telen, MJ;Chi, JT; | Phase 1 Study of a Sulforaphane-Containing Broccoli Sprout Homogenate for Sickle Cell Disease | PloS one | 27071063 | preferably the same time of day, once daily for 21 days. Patients consumed one (50g), two (100g) or three (150g) packages, depending on the dose. Cell culture Mobilized peripheral blood CD34+ cells (AllCells) were grown in StemLine II media containi | Full Paper |
2016 | Maifeld, SV;Ro, B;Mok, H;Chu, M;Yu, L;Yamagata, R;Leonardson, T;Chio, V;Parhy, B;Park, S;Carlson, M;Machhi, S;Ulbrandt, N;Falsey, AR;Walsh, EE;Wang, CK;Esser, MT;Zuo, F; | Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus | PloS one | 27070145 | serum samples showing a ≥4-fold rise in at least one ELISA assay. Thirty pediatric serum samples were purchased from Bioreclamation (Hicksville, NY). An adult human plasma sample was purchased from AllCells (Emeryville, CA) and subsequently used as t | Full Paper |
2016 | Smith, SH;Peredo, CE;Takeda, Y;Bui, T;Neil, J;Rickard, D;Millerman, E;Therrien, JP;Nicodeme, E;Brusq, JM;Birault, V;Viviani, F;Hofland, H;Jetten, AM;Cote-Sierra, J; | Development of a Topical Treatment for Psoriasis Targeting RORγ: From Bench to Skin | PloS one | 26870941 | ions were carried out in triplicate. Sequences of primers for ChIP-qPCR are listed in S1 Appendix. Human peripheral blood CD4+ T cell cultures and cytokine analysis Cryopreserved human CD4+ T cells (AllCells, LLC and Stemcell Technologies, Inc.) wer | Full Paper |
2016 | Toby, GG;Liu, T;Buyue, Y;Zhang, X;Bitonti, AJ;Pierce, GF;Sommer, JM;Jiang, H;Peters, RT; | Recombinant Factor IX Fc Fusion Protein Maintains Full Procoagulant Properties and Exhibits Prolonged Efficacy in Hemophilia B Mice | PloS one | 26840952 | e preparation (L-α-phosphatidylserine [PS] and L-α-phosphatidylcholine [PC]) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Fresh platelets from normal volunteers were purchased from AllCells, LLC (Emeryville, CA, USA). The mini-extrud | Full Paper |
2016 | Rebbapragada, I;Birkus, G;Perry, J;Xing, W;Kwon, H;Pflanz, S; | Molecular Determinants of GS-9620-Dependent TLR7 Activation | PloS one | 26784926 | Human PBMCs were isolated from fresh whole blood derived from healthy human volunteers (AllCells) using standard Ficoll density gradient separation techniques. PBMCs were Save | Full Paper |
2016 | Matsushita, M;Otsuka, Y;Tsutsumida, N;Tanaka, C;Uchiumi, A;Ozawa, K;Suzuki, T;Ichikawa, D;Aburatani, H;Okamoto, S;Kawakami, Y;Hattori, Y; | Identification of Novel HLA-A*24:02-Restricted Epitope Derived from a Homeobox Protein Expressed in Hematological Malignancies | PloS one | 26784514 | D, Oslo, Norway) and cryopreserved in Cell Banker reagent (Juji-Field, Tokyo, Japan) in liquid nitrogen until further use. Bone marrow mononuclear cells (BMMNC) from healthy donors were obtained from AllCells, LLC. (Alameda, CA). This study was appro | Full Paper |
2016 | Manser, M; | Influence of extremely-low-frequency magnetic fields on epigenetic programming and cellular differentiation | Thesis | trichostatin A (TSA). CD34-positive cells isolated from human cord blood of mixed donors were obtained from AllCells (Alabama, United States). CD34+ cells were propagated in Stemline II medium expansion medium (Stemcell technology) supplemented with | Full Paper | |
2016 | Cabrero, M;Wei, Y;Yang, H;Ganan-Gomez, I;Bohannan, Z;Colla, S;Marchesini, M;Bravo, GM;Takahashi, K;Bueso-Ramos, C;Garcia-Manero, G; | Down-regulation of EZH2 expression in myelodysplastic syndromes | Leukemia research | 26970171 | Bone marrows from healthy individuals were obtained from AllCells (Emeryville, CA). Bone marrow mononuclear cells were isolated using a standard Ficoll gradient approach. Isolation Save | Full Paper |
2016 | Salzano, G;Zappavigna, S;Luce, A;D'Onofrio, N;Balestrieri, ML;Grimaldi, A;Lusa, S;Ingrosso, D;Artuso, S;Porru, M;Leonetti, C;Caraglia, M;De Rosa, G; | Transferrin-Targeted Nanoparticles Containing Zoledronic Acid as a Potential Tool to Inhibit Glioblastoma Growth | Journal of biomedical nanotechnology | 27301207 | Viable human bone marrow cells (purchased as frozen vials from AllCells via StemCell Technologies) were prepared with a DNase treatment (Stem Cell Technologies) and then Save | Full Paper |
2016 | Phillips, JE;Renteria, L;Burns, L;Harris, P;Peng, R;Bauer, CM;Laine, D;Stevenson, CS; | Btk Inhibitor RN983 Delivered by Dry Powder Nose-only Aerosol Inhalation Inhibits Bronchoconstriction and Pulmonary Inflammation in the Ovalbumin Allergic Mouse Model of Asthma | Journal of aerosol medicine and pulmonary drug delivery | 27111445 | Mast cells One million human cord blood derived CD34+ hematopoietic stem cells (HSCs) from different donors (AllCells #CB008F-S, Emeryville, CA) were cultured for 8 weeks in a serum-free complete medium (StemPro-34 with supplements; Invitrogen, Carls | Full Paper |
2016 | Wang, X;Zhao, S;Su, M;Sun, L;Zhang, S;Wang, D;Liu, Z;Yuan, Y;Liu, Y;Li, Y; | Geraniol improves endothelial function by inhibiting NOX-2 derived oxidative stress in high fat diet fed mice | Biochemical and biophysical research communications | 27107694 | In the present study, the in vivo diet induced obese setting was reproduced in vitro by exposing HUVECs to palmitic acid (PA), the most common circulating free fatty acid, with or without geraniol. Human umbilical vein endothelial cells (HUVECs) were | Full Paper |
2016 | Xia, T;Guan, W;Fu, J;Zou, X;Han, Y;Chen, C;Zhou, L;Zeng, C;Wang, WE; | Tirofiban induces vasorelaxation of the coronary artery via an endothelium-dependent NO-cGMP signaling by activating the PI3K/Akt/eNOS pathway | Biochemical and biophysical research communications | 27018249 | Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCELLS (Shanghai, China). HUVECs were cultured at 37 °C on gelatin-coated plates in the basal nutrient media MCDB-131 supplemented with 5% fetal bovine serum (FBS; Hyclone, Logan, | Full Paper |
2016 | Cao, Z;Wang, H;Mao, X;Luo, L; | Noncanonical function of threonyl-tRNA synthetase regulates vascular development in zebrafish | Biochemical and biophysical research communications | 26993167 | Human umbilical vein endothelial cells (HUVECs, ALLCELLS) were raised according to standard protocol. Cell immunostaining was performed as previously described [15] using Save | Full Paper |
2016 | Sugino, N;Miura, Y;Yao, H;Iwasa, M;Fujishiro, A;Fujii, S;Hirai, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T; | Early osteoinductive human bone marrow mesenchymal stromal/stem cells support an enhanced hematopoietic cell expansion with altered chemotaxis- and adhesion-related gene expression profiles | Biochemical and biophysical research communications | 26707642 | Normal human BM samples that were obtained from healthy adult volunteers with informed consent were purchased from AllCells (Emeryville, CA). Human BM-MSCs were isolated and cultured based on a previously published method [3], [12], [13], [14], [15]. | Full Paper |
2016 | Koestler, DC;Jones, MJ;Usset, J;Christensen, BC;Butler, RA;Kobor, MS;Wiencke, JK;Kelsey, KT; | Improving cell mixture deconvolution by identifying optimal DNA methylation libraries (IDOL) | BMC bioinformatics | 26956433 | Purified granulocytes, monocytes, CD4T, CD8T, natural killer cells, and B cells from normal human subjects were purchased from AllCells LLC (Emeryville, CA). As described (http://www Save | Full Paper |
2016 | Sarkar, S;Sabhachandani, P;Stroopinsky, D;Palmer, K;Cohen, N;Rosenblatt, J;Avigan, D;Konry, T; | Dynamic analysis of immune and cancer cell interactions at single cell level in microfluidic droplets | Biomicrofluidics | 27795747 | RPMI-8226 (multiple myeloma (MM) cell line) was purchased from the American Type Culture Collection (ATCC, Manassas, VA). CD8+ T cells were purchased from AllCells (#PB009-3F, AllCells, Alameda, CA). Cells were maintained in RPMI-1640 medium suppleme | Full Paper |
2016 | Kikushige, Y;Akashi, K; | A TIM-3/GAL-9 autocrine stimulatory loop drives self-renewal of human myeloid leukemia stem cells and leukemic progression | Experimental Hematology | Human adult BM and PB samples were obtained from healthy donors or purchased from AllCells. CB cells were obtained from full-term deliveries (provided by the Kyushu Block Red Save | Full Paper | |
2016 | Itokazu, M;Wakitani, S;Mera, H;Tamamura, Y;Sato, Y;Takagi, M;Nakamura, H; | Transplantation of Scaffold-Free Cartilage-Like Cell-Sheets Made from Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair: A Preclinical Study | Cartilage | 27688844 | hBMSCs were prepared from three male donors aged 21 (#1), 27 (#2), and 31 (#3) years and from one commercially available bone marrow (Allcells LLC, Alameda, CA, USA) from a 20-year-old male (#4). The donor cells were derived from bone marrow aspirate | Full Paper |
2016 | Yan, Z;Liu, J;Xie, L;Liu, X;Zeng, Y; | Role of heparan sulfate in mediating CXCL8-induced endothelial cell migration | PeerJ | 26870616 | Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells, China. HUVECs were grown in complete culture media for HUVECs (Allcells, China) in a humidified 5%/ Save | Full Paper |
2016 | Hara, C;Kubota, S;Nishida, T;Hiasa, M;Hattori, T;Aoyama, E;Moriyama, Y;Kamioka, H;Takigawa, M; | Involvement of multiple CCN family members in platelets that support regeneration of joint tissues | Modern rheumatology | 26915735 | Human platelets from a healthy donor were provided by AllCells LLC (Berkley, CA). Protein extraction from the cells was performed with RIPA buffer (50 mM Tris-HCl, 0.15 M NaCl, 4 Save | Full Paper |
2016 | Zhang, M;Jiang, L; | Oxidized low-density lipoprotein decreases VEGFR2 expression in HUVECs and impairs angiogenesis | Experimental and therapeutic medicine | 28105106 | Cell culture and LDL treatment In total 2 × 105 HUVECs (AllCells, LLC, Shanghai, China) were cultured for 3-5 passages in Dulbecco's modified Eagle's medium (DMEM; PromoCell GmbH, Heidelberg, Germany) supplemented with 10% fetal bovine serum (unless | Full Paper |
2016 | Heng, BC;Zhu, S;Xu, J;Yuan, C;Gong, T;Zhang, C; | Effects of decellularized matrices derived from periodontal ligament stem cells and SHED on the adhesion, proliferation and osteogenic differentiation of human dental pulp stem cells in vitro | Tissue & cell | 26796232 | Human DPSC (Cat No. DP003F) and SHED (Cat No. DP004F) were obtained from AllCells LLC. (Alameda, CA, USA). Human PDLSC were obtained as a gift from the School of Stomatology of Fujian Medical School (Fujian, China) and were isolated as previously des | Full Paper |
2016 | Klingelhöffer, C;Reck, A;Morsczeck, C; | The extracellular concentration of osteocalcin decreased in dental follicle cell cultures during biomineralization | Cytotechnology | 27447177 | Human dental follicle cells (DFCs) were purchased from ALLCells (Alameda, CA, USA). DFCs were cultivated in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, Munich, Germany), supplemented with 10 % fetal bovine serum, FBS (Sigma-Aldrich) and 1 | Full Paper |
2016 | Ahmed, HM;Luddin, N;Kannan, TP;Mokhtar, KI;Ahmad, A; | Chemical analysis and biological properties of two different formulations of white portland cements | Scanning | 26382064 | DPSCs cell line (AllCells, USA) obtained from biopsies of the dental pulp of adult teeth was (BCGM) (AllCells, USA), with 50 mL of human MSC stimulatory supplements (AllCells, USA). Save | Full Paper |
2016 | Mulato, A;Hansen, D;Thielen, A;Porter, D;Stepan, G;White, K;Daeumer, M;Cihlar, T;Yant, SR; | Rapid In Vitro Evaluation of Antiretroviral Barrier to Resistance at Therapeutic Drug Levels | AIDS research and human retroviruses | 27356854 | Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh leukopaks obtained from healthy volunteers under informed consent (AllCells, Inc., Alameda, CA) and Save | Full Paper |
2016 | Kowolik, CM;Lin, M;Xie, J;Overman, LE;Horne, DA; | NT1721, a novel epidithiodiketopiperazine, exhibits potent in vitro and in vivo efficacy against acute myeloid leukemia | Oncotarget | 27863389 | We then tested the effect of NT1721 on primary AML samples (from AllCells, obtained from two newly diagnosed AML patients with white blood cell counts > 10 11 /L) by treating the Save | Full Paper |
2016 | Jochems, C;Hodge, JW;Fantini, M;Fujii, R;Morillon, YM;Greiner, JW;Padget, MR;Tritsch, SR;Tsang, KY;Campbell, KS;Klingemann, H;Boissel, L;Rabizadeh, S;Soon-Shiong, P;Schlom, J; | An NK cell line (haNK) expressing high levels of granzyme and engineered to express the high affinity CD16 allele | Oncotarget | 27861156 | um iodide (AO/PI) staining on a Cellometer Auto 2000 (Nexcelom, San Mateo, CA), according to the manufacturer's instructions. Release of IL-2 into culture supernatants was measured with an ELISA kit (AllCells, Alameda, CA). Antibodies and flow cytom | Full Paper |
2016 | Gołąb, K;Grose, R;Trzonkowski, P;Wickrema, A;Tibudan, M;Marek-Trzonkowska, N;Matosz, S;Solomina, J;Ostrega, D;Michael Millis, J;Witkowski, P; | Utilization of leukapheresis and CD4 positive selection in Treg isolation and the ex-vivo expansion for a clinical application in transplantation and autoimmune disorders | Oncotarget | 27821811 | We obtained de-identified human cell leukapheresis product for the all experiments from the commercial vendor AllCells, Alameda, CA, USA, therefore the study was exempt from the Save | Full Paper |
2016 | Yang, H;Sun, J;Wang, F;Li, Y;Bi, J;Qu, T; | Umbilical cord-derived mesenchymal stem cells reversed the suppressive deficiency of T regulatory cells from peripheral blood of patients with multiple sclerosis in a co-culture - a preliminary study | Oncotarget | 27705922 | RRMS, n=12, female=8, male=4), mean age 53.75 years old (49 years to 64 years old) and healthy donors (n=10, female=4, male=6), mean age 28.38 years old (22 years to 39 years old) were purchased from AllCells Inc (Emeryville, CA) or StemExpress (Plac | Full Paper |
2016 | Xu, Y;Wang, K;Yu, Q; | FRMD6 inhibits human glioblastoma growth and progression by negatively regulating activity of receptor tyrosine kinases | Oncotarget | 27661120 | entific. Secondary anti-rabbit Alexa Fluor 594 and anti-mouse Alexa Fluor 488 were from Invitrogen. Cells, GBM cell lines, and primary GBM cells Normal human astrocytes (NHAs) were obtained from ALLCELLS, Inc and ScienCell. SF126, SF188, SF210, SF | Full Paper |
2016 | Ferluga, S;Tomé, CM;Herpai, DM;D'Agostino, R;Debinski, W; | Simultaneous targeting of Eph receptors in glioblastoma | Oncotarget | 27494882 | r Center of Wake Forest University). Stacked 2D images were obtained in some cases. Images were processed with OlyVIA V2.8. Dendritic cells Myeloid-derived naïve dendritic cells were purchased from AllCells LLC (Alameda, CA). Cells were grown in RPM | Full Paper |
2016 | Sumbayev, VV;Gonçalves Silva, I;Blackburn, J;Gibbs, BF;Yasinska, IM;Garrett, MD;Tonevitsky, AG;Ushkaryov, YA; | Expression of functional neuronal receptor latrophilin 1 in human acute myeloid leukaemia cells | Oncotarget | 27322212 | oetal calf serum, penicillin (50 IU/ml) and streptomycin sulphate (50 μg/ml). Primary human AML cells Primary human AML mononuclear cells (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordan | Full Paper |
2016 | Wang, Y;Zhan, Z;Jiang, X;Peng, X;Shen, Y;Chen, F;Ji, Y;Liu, W;Shi, Y;Duan, W;Ding, J;Ai, J;Geng, M; | Simm530, a novel and highly selective c-Met inhibitor, blocks c-Met-stimulated signaling and neoplastic activities | Oncotarget | 27191264 | rom Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine (Shanghai, China). SK-OV-3 was obtained from Fudan University (Shanghai, China). HUVEC was obtained from AllCells Bio (Shanghai, China). Cells were routine | Full Paper |
2016 | Ye, BG;Sun, HC;Zhu, XD;Chai, ZT;Zhang, YY;Ao, JY;Cai, H;Ma, DN;Wang, CH;Qin, CD;Gao, DM;Tang, ZY; | Reduced expression of CD109 in tumor-associated endothelial cells promotes tumor progression by paracrine interleukin-8 in hepatocellular carcinoma | Oncotarget | 27121053 | (Sigma-Aldrich, St. Louis, MO, USA) in a humidified atmosphere of 5% CO2 at 37°C. Human normal liver L-02 cells (Chinese Academy of Sciences) were cultured under the same conditions. Primary HUVEC (AllCells, Shanghai, China) were cultured in endothel | Full Paper |
2016 | Schwaederle, M;Husain, H;Fanta, PT;Piccioni, DE;Kesari, S;Schwab, RB;Banks, KC;Lanman, RB;Talasaz, A;Parker, BA;Kurzrock, R; | Detection rate of actionable mutations in diverse cancers using a biopsy-free (blood) circulating tumor cell DNA assay | Oncotarget | 26848768 | yzed for 54 genes via NGS in circulating tumor, cell-free DNA. RESULTS Analysis of control samples from healthy persons During the technology development process, 79 healthy normal controls (source: AllCells, http://www.allcells.com) were tested and | Full Paper |
2016 | Li, Y;Wang, Y;Li, L;Kong, R;Pan, S;Ji, L;Liu, H;Chen, H;Sun, B; | Hyperoside induces apoptosis and inhibits growth in pancreatic cancer via Bcl-2 family and NF-κB signaling pathway both in vitro and in vivo | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | 26676634 | Human umbilical vein endothelial cell line (HUVEC) was obtained from all cells (Shanghai, China) and were cultured in complete medium (HUVEC-004, supplied by AllCells). All cells Save | Full Paper |
2016 | Aizman, I;Holland, WS;Yang, C;Bates, D; | αSMA Expression in Large Colonies of Colony-Forming Units-Fibroblast as an Early Predictor of Bone Marrow MSC Expandability | Cell medicine | 28003933 | The three lots that were produced from BM aspirates obtained at AllCells and, coincidentally, from older donors (see Materials and Methods) were all in the slow-growing group; however Save | Full Paper |
2016 | Liu, W;Lu, Y;Chai, X;Liu, X;Zhu, T;Wu, X;Fang, Y;Liu, X;Zhang, X; | Antitumor activity of TY-011 against gastric cancer by inhibiting Aurora A, Aurora B and VEGFR2 kinases | Journal of experimental & clinical cancer research : CR | 27887633 | Human umbilical vein endothelial cell (HUVEC) obtained from Allcells (Emeryville, CA, USA) were kept in HUVEC basic medium supplemented with 10% FBS (Allcells, Emeryville, CA, Save | Full Paper |
2016 | Han, X;Medeiros, LJ;Zhang, YH;You, MJ;Andreeff, M;Konopleva, M;Bueso-Ramos, CE; | High Expression of Human Homologue of Murine Double Minute 4 and the Short Splicing Variant, HDM4-S, in Bone Marrow in Patients With Acute Myeloid Leukemia or Myelodysplastic Syndrome | Clinical lymphoma, myeloma & leukemia | 27155969 | cDNAs from purified normal CD33+ bone marrow myeloid cells, CD34+ hematopoietic progenitor cells, CD71+ normoblasts, and CD19+ circulating peripheral blood B cells were purchased from AllCells, LLC (Emeryville, CA) for reverse transcriptase-polymeras | Full Paper |
2016 | Ali, S; | Cell Line Cross-Contamination and Accidental Co-Culture | Journal of Stem Cell Research & Therapeutics | a https://www.abmgood.com/ Custom-Cell-Authentication-Service.html STR Profiling Analytical Biological Services Inc, USA http://www.absbio.com/products /cellQC/celllineauthen.html STR profiling AllCells, California www.allcells.com - Asteran | Full Paper | |
2016 | Dou, W;Cheung, S;Gao, C;Xu, C;Xu, L;Wei, J; | Detecting table clones and smells in spreadsheets | Proceedings of the 2016 24th ACM SIGSOFT International Symposium on Foundations of Software Engineering | TableCheck's clone detection algorithm (Algorithm 1) takes the lookup table and all cells (allCells) Then all cells in this group are removed from allCells (Lines 14-18). (5) The algorithm Save | Full Paper | |
2016 | DeWitt, M;Magis, W;Bray, N;Wang, T;Berman, J;Urbinati, F;Muñoz, D;Kohn, D;Walters, M;Carroll, D;Martin, D;Corn, J; | Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein Complex | bioRxiv | Cryopreserved WT human mobilized peripheral blood CD34+ HSPCs were purchased from Allcells, Inc. SCD CD34+ HSPCs were prepared by Allcells Inc. from whole blood obtained Save | Full Paper | |
2016 | Meader, ES; | Understanding the role of miRNA expression in the differentiation of haematopoietic stem cells from pluripotent stem cells | Thesis | not been released in spite of several requests, but the sequences of the miRNAs tested are shown in Table 2.4. Small nuclear RNAs RNU44 and RNU48 were chosen as internal controls as they are known to be stably and abundantly expressed across differen | Full Paper | |
2016 | Tran, R; | A microfluidics-based paradigm for clinical lentivector gene transfer | Thesis | Frozen human pan T cells were purchased from AllCells (AllCells, LLC., Alameda, CA, USA). Cells were thawed following the AllCells thawing protocol and allowed to recover in RPMI 1640 media supplemented with 10% FBS, L-Glutamine, 25 mM HEPES, and 1% | Full Paper | |
2016 | Abu Samra, DBK; | Molecular Characterization of the Interactions between Vascular Selectins and Glycoprotein Ligands on Human Hematopoietic Stem/Progenitor Cells | Thesis | bone marrow (CD34+ -bone marrow), AML derived bone marrow (from patients with newly diagnosed or relapsed AML; AML bone marrow-CD34+ ) or the mononuclear fraction of whole bone marrow were purchased from ALL CELLS (Alameda, CA, USA; www.allcells.com) | Full Paper | |
2016 | Ma, L; | Targeting Drug Resistance in Chronic Myeloid Leukemia: A Dissertation | Thesis | in FBS + 10% DMSO, frozen slowly overnight, and then transferred to liquid nitrogen for long-term storage. All samples were provided in a de-identified manner. Samples from normal donors were purchased from a commercial vendor (AllCells or Lonza) | Full Paper | |
2016 | Liu, X; | Functional and structural study of the AHI-1 SH3 domain, characterization of the BCR-ABL-AHI-1-Dynamin-2 protein complex and investigation of oncogenic roles of dynamin-2 in chronic myeloid leukemia | Thesis | Primary CML samples were obtained from newly-diagnosed CP-CML patients before any TKI intervention, and BM cells were obtained from health donors (ALLCELLS, Alameda, CA). The subsequent IM-responders were classified based on the European Leukemia Net | Full Paper | |
2016 | Ferreira, L; | Transcriptional Control of Maternal-Fetal Immune Tolerance | Thesis | Primary CD4+ T cells were isolated from peripheral blood (Leukopacs, MGH) using RosetteSep CD4+ T cell enrichment cocktail (STEMCELL Technologies). CD34+ cells from G-CSF mobilized peripheral blood were purchased from AllCells | Full Paper | |
2016 | Coronado, RE; | The effect of decellularized liver matrix on stem cell differentiation into hepatocyte-like cells | Thesis | Bone Marrow Derived Mesenchymal Stromal/Stem Cells: Bone marrow derived mesenchymal stem cells (BM-MSC) were isolated from whole bone marrow aspirates purchased from AllCells Alameda, CA from donors less than 30 years old. The isolation protocol was | Full Paper | |
2016 | Gangolli, R; | A novel biomimetic scaffold for guided tissue regeneration of the pulp-dentin complex | Thesis | DPSC from AllCells at Passage P5 were used for the study. DPSC-P5 were cultured on T175 flasks using proliferation media with MEM Alpha, 15% FBS and 1% Pen-strep which had been filtered through a 0.2 um filter flask. Culture conditions were maintaine | Full Paper | |
2016 | Bjornson-Hooper, ZB; | Cross-Species Immune System Atlas | Thesis | Venous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human subjects research) or from volunteers from the Stanford community under an IRB-approved protocol. Baboon (Papio hamadryas anubis x Papio ha | Full Paper | |
2016 | Varma, D; | Development of a Redox-Crosslinked Cellulose-Based Hydrogel for Nucleus Pulposus Replacement and Repair | Thesis | hMSCs were isolated from adult human bone marrow (AllCells, Emeryville, CA) via enriched density gradient centrifugation as previously described (Gupta et al., 2011; Gupta and Nicoll, 2014b). Briefly, an hMSC Enrichment Cocktail (StemCell Technologie | Full Paper | |
2016 | Sivajothi, SK; | Comparative properties of stromal cells and their role in tissue regeneration | Thesis | 2.1 Apheresis Products Non-mobilized PBPC and G-CSF mobilized PBPC were purchased from Allcells LLC (Emeryville, CA): 2.2 Cells and Cell Culture Human bone marrow MSCs were isolated by differential adhesion from bonemarrow aspirate obtained from huma | Full Paper | |
2016 | Fragiadakis, GK; | Profiling Human Immune Variation at the Systems-Level in Clinical Contexts and at Steady-State | Thesis | Blood Venous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human subjects research) or from volunteers from the Stanford community under an IRB-approved protocol. Antibodies Purified antibodies were | Full Paper | |
2016 | Fried, ES; | Applications of Proteolipobeads in Biomimetic Cellular Interactions And Lipid Phase Separation | Thesis | N-Diisopropylethylamine (DIEA), piperidine, carboxymethylcellulose (CMC), and methacrylic anhydride were purchased from Sigma Aldrich (St. Louis, MO). Human mesenchymal stem cells (MSCs) isolated from bone marrow extracted from a 24-year-old male wer | Full Paper | |
2016 | Lucera, MB; | Lysine Acetylation and Small Molecule Epigenetic Inhibition Reveal Novel Mechanisms Controlling Cellular Susceptibility to HIV-1 Infection | Thesis | CD4+ T cells were obtained by leukopheresis from ALLCELLS, LLC. All subjects were healthy control donors negative for HBV, HCV, and HIV. PBMCs were isolated by ficoll gradient separation while CD4+ T cells were purified by adding RosetteSep CD4+ T ce | Full Paper | |
2016 | Milford, TA; | Comparative Studies of TSLP and IL-7 in Normal Early Human Neonatal and Adult B Cell Development | Thesis | CB was collected according to guidelines approved by the Institutional Review Boards at Children’s Hospital Los Angeles and Loma Linda University. Adult human BM was purchased from AllCells, LLC, Berkeley, CA; the National Disease Research Interchang | Full Paper | |
2016 | Richter, M;Saydaminova, K;Yumul, R;Krishnan, R;Liu, J;Nagy, EE;Singh, M;Izsvák, Z;Cattaneo, R;Uckert, W;Palmer, D;Ng, P;Haworth, KG;Kiem, HP;Ehrhardt, A;Papayannopoulou, T;Lieber, A; | In vivo transduction of primitive mobilized hematopoietic stem cells after intravenous injection of integrating adenovirus vectors | Blood | 27554082 | Human CD34+-enriched cells from G-CSF mobilized normal donors were obtained from the Fred Hutchinson Cancer Research Center Cell Processing Core Facility and cultured as described previously.8 Colony-forming unit (CFU) assays were performed using Col | Full Paper |
2016 | Castillo, J;Raisner, R;Gascoigne, K;Dos Santos, G;Clarke, E;Huang, A;Lackner, M;Mounir, Z; | Abstract 4516: Evaluating the chromatin state as a predictive biomarker for BET inhibitor sensitivity in hematological malignancies | Cancer Research | 1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++2ReachBio, WA.++2ReachBio, WA.++1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++1Genentech, South San Franci | Full Paper | |
2016 | Ferguson, GD;Delgado, M;Plantevin-Krenitsky, V;Jensen-Pergakes, K;Bates, RJ;Torres, S;Celeridad, M;Brown, H;Burnett, K;Nadolny, L;Tehrani, L;Packard, G;Pagarigan, B;Haelewyn, J;Nguyen, T;Xu, L;Tang, Y;Hickman, M;Baculi, F;Pierce, S;Miyazawa, K;Jackson, P;Chamberlain, P;LeBrun, L;Xie, W;Bennett, B;Blease, K; | A Novel Triazolopyridine-Based Spleen Tyrosine Kinase Inhibitor That Arrests Joint Inflammation | PloS one | 26756335 | ature in the dark. Fluorescence readings at 460 nm and 530 nm were taken using the Flexstation II (Molecular Devices). Myeloid colony forming assays to assess Jak2 cellular activity were conducted at ReachBio (Seattle, WA). Ramos NFAT β-lactamase re | Full Paper |
2016 | Hariharan, S; | STUDY OF GROWTH OF STRESS ERYTHROID PROGENITORS FROM PERIPHERAL BLOOD MONONUCLEAR CELLS IN MURINE AND HUMAN ANEMIC MODEL SYSTEMS | Thesis | The control Peripheral Blood samples were obtained from ReachBio Labs. The PBMCs were then isolated from peripheral blood using Histopaque 1077 using density gradient. The Save | Full Paper | |
2016 | Holzapfel, N; | Investigating the Role of the RNA-Binding Protein MUSASHI-2 (MSI2) in Normal Hematopoiesis and Leukemia | Thesis | AML*CFU%assays%were%performed%in%semi*solid%ColonyGel%media%(Human%Complete% Media% 1102;% reachBio)% with% flow*sorted% GFP+%MSI2% knockdown% AML% cells.% 10000% GFP+%cells%were%plated%in%duplicate%in%each%well%of%a%24*well%plate%and%loose%colonies% | Full Paper | |
2015 | Sun, LL;Ellerman, D;Mathieu, M;Hristopoulos, M;Chen, X;Li, Y;Yan, X;Clark, R;Reyes, A;Stefanich, E;Mai, E;Young, J;Johnson, C;Huseni, M;Wang, X;Chen, Y;Wang, P;Wang, H;Dybdal, N;Chu, YW;Chiorazzi, N;Scheer, JM;Junttila, T;Totpal, K;Dennis, MS;Ebens, AJ; | Anti-CD20/CD3 T cell-dependent bispecific antibody for the treatment of B cell malignancies | Sci Transl Med | 25972002 | We have obtained 40 ml of whole blood collected in Streck tubes, separated into plasma and extracted cfRNA samples from two Stage IV Prostate cancer patients and three healthy controls from Conversant Bio and generated cfRNA sequencing data with UMIs | Full Paper |
2015 | Li, L;Lyu, X;Hou, C;Takenaka, N;Nguyen, HQ;Ong, CT;Cubeñas-Potts, C;Hu, M;Lei, EP;Bosco, G;Qin, ZS;Corces, VG; | Widespread rearrangement of 3D chromatin organization underlies polycomb-mediated stress-induced silencing | Mol. Cell | 25818644 | We thank Drs. John T. Lis for providing antibodies against RNAPII, and Vincent Pirrotta and Richard Jones for providing antibodies against Pc. We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology, and special | Full Paper |
2015 | Bhate, A;Parker, DJ;Bebee, TW;Ahn, J;Arif, W;Rashan, EH;Chorghade, S;Chau, A;Lee, JH;Anakk, S;Carstens, RP;Xiao, X;Kalsotra, A; | ESRP2 controls an adult splicing programme in hepatocytes to support postnatal liver maturation | Nat Commun | 26531099 | ... RI digested amplified PCR products for Esrp2 cDNA in pDP19 vector (Ambion). Sense and anti-sense RNA strands were generated as previously described43. In situ hybridization for Esrp2 was performed by Phylogeny Inc. (Columbus, Ohio.) The C57BL/6 m | Full Paper |
2015 | Weckselblatt, B;Hermetz, KE;Rudd, MK; | Unbalanced translocations arise from diverse mutational mechanisms including chromothripsis | Genome Res. | 26070663 | We used Agilent SureSelect Target Enrichment to pull down 40-kb regions around breakpoints fine-mapped by custom array CGH. SureSelect followed by Illumina HiSeq sequencing was performed at HudsonAlpha Genomic Services Laboratory, and sequence analys | Full Paper |
2015 | Jagannathan, S;Abdel-Malek, MA;Malek, E;Vad, N;Latif, T;Anderson, KC;Driscoll, JJ; | Pharmacologic screens reveal metformin that suppresses GRP78-dependent autophagy to enhance the anti-myeloma effect of bortezomib | Leukemia | 26108695 | Eagle's medium. Bone marrow aspirates were obtained after approval institutional review board or from ConversantBio (Huntsville, AL, USA) and CD138 + cells then isolated (Miltenyi Biotec, San Diego, CA, USA). Side population | Full Paper |
2015 | Jagannathan, S;Vad, N;Vallabhapurapu, S;Vallabhapurapu, S;Anderson, KC;Driscoll, JJ; | MiR-29b replacement inhibits proteasomes and disrupts aggresome+autophagosome formation to enhance the antimyeloma benefit of bortezomib | Leukemia | 25234165 | (c) Effect of control or miR-29b on myeloma patient tumor cells. CD138 + PCs from bone marrow (BM; ConversantBio, Huntsville, AL, USA) were isolated using microbead-positive selection (Miltenyi Biotec, Auburn, CA, USA) | Full Paper |
2015 | Blume-Jensen, P;Berman, DM;Rimm, DL;Shipitsin, M;Putzi, M;Nifong, TP;Small, C;Choudhury, S;Capela, T;Coupal, L;Ernst, C;Hurley, A;Kaprelyants, A;Chang, H;Giladi, E;Nardone, J;Dunyak, J;Loda, M;Klein, EA;Magi-Galluzzi, C;Latour, M;Epstein, JI;Kantoff, P;Saad, F; | Development and clinical validation of an in situ biopsy-based multimarker assay for risk stratification in prostate cancer | Clin. Cancer Res. | 25733599 | To develop a robust assay, multiple institutions were recruited representing typical North American patient cohorts (Table 1): Urology Austin, Chesapeake Urology Associates, Cleveland Clinic, Michigan Urology, and Folio Biosciences. Biopsy sample inc | Full Paper |
2015 | Garcia-Manero, G;Khoury, HJ;Jabbour, E;Lancet, J;Winski, SL;Cable, L;Rush, S;Maloney, L;Hogeland, G;Ptaszynski, M;Calvo, MC;Bohannan, Z;List, A;Kantarjian, H;Komrokji, R; | A phase I study of oral ARRY-614, a p38 MAPK/Tie2 dual inhibitor, in patients with low or intermediate-1 risk myelodysplastic syndromes | Clin. Cancer Res. | 25480830 | ... s (Aurora, CO) proprietary CellMap customized algorithm for p-p38 and CC3, respectively. Age-defined FFPE bone marrow biopsies from apparently healthy subjects (60–74 years old) were obtained from Folio Biosciences (Columbus, OH). Plasma was iso | Full Paper |
2015 | Grabek, KR;Diniz Behn, C;Barsh, GS;Hesselberth, JR;Martin, SL; | Enhanced stability and polyadenylation of select mRNAs support rapid thermogenesis in the brown fat of a hibernator | Elife | 25626169 | Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, United States.++Department of Applied Math and Statistics, Colorado School of Mines, Golden, United States.++Department of Research, HudsonAlpha Institut | Full Paper |
2015 | Li, C;Gowan, S;Anil, A;Beck, BH;Thongda, W;Kucuktas, H;Kaltenboeck, L;Peatman, E; | Discovery and validation of gene-linked diagnostic SNP markers for assessing hybridization between Largemouth bass (Micropterus salmoides) and Florida bass (M. floridanus) | Mol Ecol Resour | 25047482 | ; of 110-140 nm. After KAPA quantitation and dilution, based on included DNA standards (1-6), the libraries were sequenced in a single lane on an Illumina HiSeq 2000 instrument with 100 bp paired‐end (PE) reads at HudsonAlpha Genomic Services Lab (H | Full Paper |
2015 | Nguyen, D;Rubinstein, L;Takebe, N;Miele, L;Tomaszewski, JE;Ivy, P;Doroshow, JH;Yang, SX; | Notch1 phenotype and clinical stage progression in non-small cell lung cancer | J Hematol Oncol | 25653136 | Lung cancer tissue microarrays and clinicopathological data were obtained from Cybrdi, Incorporation (Rockville, MD), and FOLIO Biosciences (Powell, OH). Approval of the biomarker study on de-identified human tissues was obtained from the Office of H | Full Paper |
2015 | Huynh, H;Hao, HX;Chan, SL;Chen, D;Ong, R;Soo, KC;Pochanard, P;Yang, D;Ruddy, D;Liu, M;Derti, A;Balak, MN;Palmer, MR;Wang, Y;Lee, BH;Sellami, D;Zhu, AX;Schlegel, R;Huang, A; | Loss of Tuberous Sclerosis Complex 2 (TSC2) Is Frequent in Hepatocellular Carcinoma and Predicts Response to mTORC1 Inhibitor Everolimus | Mol. Cancer Ther. | 25724664 | HCC cell lines and patient-derived xenografts were collected, fixed with formalin and processed for paraffin embedding. HCC tissue microarrays were purchased from BioChain (catalog # Z7020056 and Z7020057, unrelated HCC TMAs) and FolioBio (catalog# A | Full Paper |
2015 | Mezache, L;Paniccia, B;Nyinawabera, A;Nuovo, GJ; | Enhanced expression of PD L1 in cervical intraepithelial neoplasia and cervical cancers | Mod. Pathol. | 26403783 | and cervical cancers Louisa Mezache 1 Bernard Paniccia 1 Angelique Nyinawabera 1 Gerard Nuovo 1,2 Phylogeny Inc. 1 , Powell, Ohio and OSU Comprehensive Cancer Center 2 , Columbus Ohio Page 2. 2 - Mezache et al Direct all correspondence to: Gerard J N | Full Paper |
2015 | Shen, J;Putt, KS;Visscher, DW;Murphy, L;Cohen, C; | Assessment of folate receptor-β expression in human neoplastic tissues | Oncotarget | ... from US Biomax (Rockville, MD), and v) a breast tumor microarray (ARY-HH0056), a brain glioma tumor microarray (ARY-HH0138) and an endometrial carcinoma progression tumor microarray (ARY-HH0211) from Folio Biosciences (Columbus, OH). Oregon green | Full Paper | |
2015 | Azad, AK;Rajaram, MV;Metz, WL;Cope, FO;Blue, MS;Vera, DR;Schlesinger, LS; | γ-Tilmanocept, a New Radiopharmaceutical Tracer for Cancer Sentinel Lymph Nodes, Binds to the Mannose Receptor (CD206) | J. Immunol. | 26202986 | We thank Phylogeny, Inc. (Powell, OH) for sectioning and processing the lymph node tissues. We also acknowledge the support of the Campus Microscopy and Imaging Facility of The Ohio State University (Columbus, OH) for confocal microscopy. | Full Paper |
2015 | Waight, JD;Takai, S;Marelli, B;Qin, G;Hance, KW;Zhang, D;Tighe, R;Lan, Y;Lo, KM;Sabzevari, H;Hofmeister, R;Wilson, NS; | Cutting edge: epigenetic regulation of Foxp3 defines a stable population of CD4+ regulatory T cells in tumors from mice and humans | J. Immunol. | 25548231 | Mouse tumor cells were isolated as described previously (12). Human tumor specimens were collected before therapy and purchased from Conversant Bio. Healthy donor PBMCs (Research Blood Components) were isolated using Ficoll-Paque density gradient. Sa | Full Paper |
2015 | Li, Y;Kocot, KM;Schander, C;Santos, SR;Thornhill, DJ;Halanych, KM; | Mitogenomics reveals phylogeny and repeated motifs in control regions of the deep-sea family Siboglinidae (Annelida) | Mol. Phylogenet. Evol. | 25721539 | Mitogenomics reveals phylogeny and repeated motifs in control regions of the deep-sea family Siboglinidae (Annelida) | Full Paper |
2015 | Cheng, A;Hernandez, N;Li, M;San Jose Hinahon, C;Magdaleno, S; | Abstract 4755: Maximize genomic analysis through dual recovery of DNA and RNA from the same clinical sample | Molecular and Cellular Biology | variant, indel data, RNA fusion detection and expression data. MATERIALS AND METHODS FFPE surgical resection and FNA blocks were purchased from Folio Biosciences. Age of blocks ranged from 1992- 2011 with various tumor stages. One 4um section mounted | Full Paper | |
2015 | Cunningham, CB;Ji, L;Wiberg, RA;Shelton, J;McKinney, EC;Parker, DJ;Meagher, RB;Benowitz, KM;Roy-Zokan, EM;Ritchie, MG;Brown, SJ;Schmitz, RJ;Moore, AJ; | The Genome and Methylome of a Beetle with Complex Social Behavior, Nicrophorus vespilloides (Coleoptera: Silphidae) | Genome Biol Evol | 26454014 | Testing for conserved and novel mechanisms underlying phenotypic evolution requires a diversity of genomes available for comparison spanning multiple independent lineages. For example, complex social behavior in insects has been investigated primaril | Full Paper |
2015 | Yamaguchi, SI;Ueki, A;Sugihara, E;Onishi, N;Yaguchi, T;Kawakami, Y;Horiuchi, K;Morioka, H;Matsumoto, M;Nakamura, M;Muto, A;Toyama, Y;Saya, H;Shimizu, T; | Synergistic antiproliferative effect of imatinib and adriamycin in platelet-derived growth factor receptor-expressing osteosarcoma cells | Cancer Sci. | 25940371 | A lung tissue section was randomly obtained from each lobe. Human OS tissue array. An array of human OS specimens was obtained from Folio Biosciences (Powell, OH, USA) and was subjected to immunohistochemical staining for PDGFRβ | Full Paper |
2015 | Zemskova, MY;Song, JH;Cen, B;Cerda-Infante, J;Montecinos, VP;Kraft, AS; | Regulation of prostate stromal fibroblasts by the PIM1 protein kinase | Cell. Signal. | 25451079 | scores. P values were calculated by t test and represent the probability of no difference between PIM1 positive cells in the stroma of normal and cancer tissues. (C) IHC staining of PIM1 using a TMA (Folio Bioscience). PIM1 | Full Paper |
2015 | Ergin, A;Großerüschkamp, F;Theisen, O;Gerwert, K;Remiszewski, S;Thompson, CM;Diem, M; | A method for the comparison of multi-platform spectral histopathology (SHP) data sets | Analyst | 25664352 | M. Miljković, et al., Spectral Cytopathology: new aspects of data collection, manipulation and confounding effects, Analyst, 2013, 138, 3975-3982 RSC . foliobio.com. Deparaffin Protocol. Available from: http://foliobio.com/media/pdf/Deparaffin%20prot | Full Paper |
2015 | Steffen, MM;Belisle, BS;Watson, SB;Boyer, GL;Bourbonniere, RA;Wilhelm, SW; | Metatranscriptomic evidence for co-occurring top-down and bottom-up controls on toxic cyanobacterial communities | Appl. Environ. Microbiol. | 25662977 | ... using the Epicentre Ribo-Zero rRNA removal kit (Epicentre, Madison, WI, USA). Secondary quality analysis and sequencing were done on the Illumina HiSeq platform for 50-bp paired-end sequencing by the HudsonAlpha Genomic Services Laboratory (Hunts | Full Paper |
2015 | Van Bortle, K;Peterson, AJ;Takenaka, N;O'Connor, MB;Corces, VG; | CTCF-dependent co-localization of canonical Smad signaling factors at architectural protein binding sites in D. melanogaster | Cell Cycle | 26125535 | llumina adaptors (Illumina Cat# PE-102-1001) were titrated according to prepared DNA ChIP sample concentration and ligated with T4 ligase (NEB Cat# M0202S). Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (NE | Full Paper |
2015 | Ritchie, SA;Chitou, B;Zheng, Q;Jayasinghe, D;Jin, W;Mochizuki, A;Goodenowe, DB; | Pancreatic cancer serum biomarker PC-594: Diagnostic performance and comparison to CA19-9 | World J. Gastroenterol. | 26074698 | MATERIALS AND METHODS. Sample cohort. PaC (n = 84) and control (n = 99) serum samples were obtained from Conversant Bio (www.conversantbio.com). All patients signed informed consents, and samples were collected | Full Paper |
2015 | Premkumar, DR;Jane, EP;Pollack, IF; | Cucurbitacin-I inhibits Aurora kinase A, Aurora kinase B and survivin, induces defects in cell cycle progression and promotes ABT-737-induced cell death in a caspase-independent manner in malignant human glioma cells | Cancer Biol. Ther. | 25482928 | We also obtained primary glioma cells from Conversant Biologics (Huntsville, AL). Established malig- nant human glioma cell lines such as T98G, A172 and LN229 were obtained from the American Type Culture Collection (Manassas, VA) | Full Paper |
2015 | Tehranchian, P;Norsworthy, JK;Nandula, V;McElroy, S;Chen, S;Scott, RC; | First report of resistance to acetolactate-synthase-inhibiting herbicides in yellow nutsedge (Cyperus esculentus): confirmation and characterization | Pest Manag. Sci. | 25307777 | To identify potential target-site mutations, massively parallel sequencing using the Illumina HiSeq (Illumina, San Diego, CA; http://www.illumina.com/) platform was utilized in lieu of traditional short-read capillary sequencing using the polymerase | Full Paper |
2015 | Campos, AH;Schreeder, M;Parry-Jones, A;Abdelhafiz, AS;Larson, D;Pruetz, B;Geddes, T;Salman, A;Lazaris, A; | Addressing the Challenge of Financial Sustainability in Biobanking | Biopreserv Biobank | 26697907 | Marshall Schreeder. Chief Executive Officer, Co-Founder, Conversant Bio, Huntsville, Alabama Conversant Bio, Huntsville, Alabama. Conversant Bio was founded in 2006 because the founders knew that the drug discovery research model was broken | Full Paper |
2015 | Chen, S;McElroy, JS;Flessner, ML;Dane, F; | Utilizing next-generation sequencing to study homeologous polymorphisms and herbicide-resistance-endowing mutations in Poa annua acetolactate synthase genes | Pest Manag. Sci. | 25180862 | Detection of single nucleotide polymorphisms (SNPs) related to herbicide resistance in non-model polyploid weed species is fraught with difficulty owing to the gene duplication and lack of reference sequences. Our research seeks to overcome these obs | Full Paper |
2015 | Cui, J;Xu, J;Zhang, S;Wang, K;Jiang, Y;Mahboob, S;Al-Ghanim, KA;Xu, P; | Transcriptional Profiling Reveals Differential Gene Expression of Amur Ide (Leuciscus waleckii) during Spawning Migration | Int J Mol Sci | 26096003 | ... nt Technologies, Santa Clara, CA, USA). Equal amounts of high quality RNA samples from the liver were then pooled for RNA-Seq analysis. RNA-Seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2015 | Baker, GM;Murphy, T;Block, T;Nguyen, D;Lynch, FJ; | Development and validation of an immunohistochemistry assay to assess glucocorticoid receptor expression for clinical trials of mifepristone in breast cancer | Cancer Manag Res | 26673410 | Tiffany Murphy. 2 QualTek Molecular Laboratories, Newtown, PA, USA. Find articles by Tiffany Murphy. Thaddeus Block Dat Nguyen. 3 Corcept Therapeutics, Menlo Park, CA, USA. Find articles by Dat Nguyen. Frank J Lynch. 2 QualTek Molecular Laboratorie | Full Paper |
2015 | Zisis, K;Piper, M;Henderson, MK; | The 2015 ISBER Annual Meeting & Exhibits in Phoenix, Arizona, USA: bridging the canyon-connecting biobank communities through innovations in global health, research and environmental preservation | Biopreserv Biobank | 25686051 | This year's session focused on the business aspects of biobank planning, and included Marshall Schreeder's (ConversantBIO) perspective from a commer- cial biobank and Clive Green's (AstraZeneca) business planning for a pharmaceutical biobank | Full Paper |
2015 | Zhao, H;Li, C;Beck, BH;Zhang, R;Thongda, W;Davis, DA;Peatman, E; | Impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings, Ictalurus punctatus | Fish Shellfish Immunol. | 26164837 | RNA quality of each sample was measured on an Agilent 2100 Bioanalyzer using the RNA Nano Bioanalysis chip. RNA-seq library preparation was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) | Full Paper |
2015 | Zhu, J;Li, C;Ao, Q;Tan, Y;Luo, Y;Guo, Y;Lan, G;Jiang, H;Gan, X; | Trancriptomic profiling revealed the signatures of acute immune response in tilapia (Oreochromis niloticus) following Streptococcus iniae challenge | Fish Shellfish Immunol. | 26117728 | ; For each timepoint, equal amounts of RNA from the three replicates were pooled for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) | Full Paper |
2015 | Foster, KA;Jane, EP;Premkumar, DR;Morales, A;Pollack, IF; | NVP-BKM120 potentiates apoptosis in tumor necrosis factor-related apoptosis-inducing ligand-resistant glioma cell lines via upregulation of Noxa and death receptor 5 | Int. J. Oncol. | 26044191 | U87 was obtained from American Type Culture Collection (Manasas, VA, USA) and LNZ308 was kindly provided by Dr Nicolas de Tribolet (Switzerland). We obtained primary GBM cells from Conversant Biologics (Huntsville, AL, USA) | Full Paper |
2015 | Baxter, A;Thain, S;Banerjee, A;Haswell, L;Parmar, A;Phillips, G;Minet, E; | Targeted omics analyses, and metabolic enzyme activity assays demonstrate maintenance of key mucociliary characteristics in long term cultures of reconstituted human airway epithelia | Toxicol In Vitro | 25863282 | The 180 PBS was collected and stored at A80 °C. Sputum from 10 non- 181 smokers (Sofia Bio, Sofia, Bulgaria, study code SB-SC_v1) and 182 MucilAir™ ASL were thawed, centrifuged and filtered using a 183 0.2 lm filter plate (PALL Corporation, Port Wash | Full Paper |
2015 | Marshall, DC;Lyman, SK;McCauley, S;Kovalenko, M;Spangler, R;Liu, C;Lee, M;O'Sullivan, C;Barry-Hamilton, V;Ghermazien, H;Mikels-Vigdal, A;Garcia, CA;Jorgensen, B;Velayo, AC;Wang, R;Adamkewicz, JI;Smith, V; | Selective Allosteric Inhibition of MMP9 Is Efficacious in Preclinical Models of Ulcerative Colitis and Colorectal Cancer | PLoS ONE | 25961845 | ... h as CRC. Materials and Methods Tissues Fresh-frozen (FF) and formalin fixed and paraffin embedded (FFPE) human tissues were obtained from Cureline, Inc. (Burlingame, CA), Asterand (Detroit, MI), or Folio Biosciences (Powell, OH). Recombinant M | Full Paper |
2015 | Hoek, KL;Samir, P;Howard, LM;Niu, X;Prasad, N;Galassie, A;Liu, Q;Allos, TM;Floyd, KA;Guo, Y;Shyr, Y;Levy, SE;Joyce, S;Edwards, KM;Link, AJ; | A cell-based systems biology assessment of human blood to monitor immune responses after influenza vaccination | PLoS ONE | 25706537 | Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, 37232, United States of America.++Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, 37232, United States | Full Paper |
2015 | Kalinsky, K;Mayer, JA;Xu, X;Pham, T;Wong, KL;Villarin, E;Pircher, TJ;Brown, M;Maurer, MA;Bischoff, FZ; | Correlation of hormone receptor status between circulating tumor cells, primary tumor, and metastasis in breast cancer patients | Clin Transl Oncol | 25613123 | Peripheral blood was collected under appropriate third party institution review board approved protocols (Columbia University Medical Center, AdeptBio, ConversantBIO and BioOptions) and delivered to Biocept's CLIA/CAP accredited laboratory | Full Paper |
2015 | Wang, Z;Lebron, JA;Wolf, JJ; | Reliable quantification of mRNA in archived formalin-fixed tissue with or without paraffin embedding | J Pharmacol Toxicol Methods | 25280797 | quantify mRNA in archived FFPE and prolonged formalin-fixed tissue. 2. Methods. 2.1. Samples. Four human FFPE liver samples were purchased from Folio Biosciences. Two mouse FFPE liver samples and two mouse formalin | Full Paper |
2015 | Rokyta, DR;Wray, KP;McGivern, JJ;Margres, MJ; | The transcriptomic and proteomic basis for the evolution of a novel venom phenotype within the Timber Rattlesnake (Crotalus horridus) | Toxicon | 25727380 | Sequencing and nonnormalized cDNA library preparation for our two adults were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially a | Full Paper |
2015 | Pawar, H;Srikanth, SM;Kashyap, MK;Sathe, G;Chavan, S;Singal, M;Manju, HC;Kumar, KV;Vijayakumar, M;Sirdeshmukh, R;Pandey, A;Prasad, TS;Gowda, H;Kumar, RV; | Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell Carcinoma | ScientificWorldJournal | 26788548 | The ESCC tumors varied from Grade I to Grade III and were obtained from patients in the age group of 36 to 78 years and (ii) FolioBio (Powell, OH; Catalog number ARY-HH0091) consisting of 40 ESCC cases with matched adjacent normal esophageal epitheli | Full Paper |
2015 | Krönke, J;Fink, EC;Hollenbach, PW;MacBeth, KJ;Hurst, SN;Udeshi, ND;Chamberlain, PP;Mani, DR;Man, HW;Gandhi, AK;Svinkina, T;Schneider, RK;McConkey, M;Järås, M;Griffiths, E;Wetzler, M;Bullinger, L;Cathers, BE;Carr, SA;Chopra, R;Ebert, BL; | Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS | Nature | 26131937 | uced cells in each condition was assessed after 5 days by flow cytometry. Control cord-blood CD34+ cells were isolated as above. Adult bone marrow CD34+ cells were purchased as single-donor lots from AllCells (Alameda, CA). The number of replicates f | Full Paper |
2015 | Hendel, A;Bak, RO;Clark, JT;Kennedy, AB;Ryan, DE;Roy, S;Steinfeld, I;Lunstad, BD;Kaiser, RJ;Wilkens, AB;Bacchetta, R;Tsalenko, A;Dellinger, D;Bruhn, L;Porteus, MH; | Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells | Nature biotechnology | 26121415 | Cas9-encoding plasmid. Mobilized human peripheral blood CD34+ HSPCs were purchased from AllCells and thawed according to manufacturer’s instructions. CD34+ HSPCs were maintained in X-VIVO 15 (Lonza) supplemented with SCF (100 ng/ml), TPO (100 ng/ml), | Full Paper |
2015 | Kotini, AG;Chang, CJ;Boussaad, I;Delrow, JJ;Dolezal, EK;Nagulapally, AB;Perna, F;Fishbein, GA;Klimek, VM;Hawkins, RD;Huangfu, D;Murry, CE;Graubert, T;Nimer, SD;Papapetrou, EP; | Functional analysis of a chromosomal deletion associated with myelodysplastic syndromes using isogenic human induced pluripotent stem cells | Nature biotechnology | 25798938 | CB CD34 + cells were purchased from AllCells. Two to four shRNAs against each of the four genes HIPK2, ATP6V0E2, LUC7L2 and EZH2 and one scramble sequence were inserted in Save | Full Paper |
2015 | Levine, JH;Simonds, EF;Bendall, SC;Davis, KL;Amir, el-AD;Tadmor, MD;Litvin, O;Fienberg, HG;Jager, A;Zunder, ER;Finck, R;Gedman, AL;Radtke, I;Downing, JR;Pe'er, D;Nolan, GP; | Data-Driven Phenotypic Dissection of AML Reveals Progenitor-like Cells that Correlate with Prognosis | Cell | 26095251 | For healthy adult controls, cryopreserved healthy BMMCs were purchased from AllCells (Emeryville, CA). All human samples were obtained with informed consent in compliance with Save | Full Paper |
2015 | Moraga, I;Wernig, G;Wilmes, S;Gryshkova, V;Richter, CP;Hong, WJ;Sinha, R;Guo, F;Fabionar, H;Wehrman, TS;Krutzik, P;Demharter, S;Plo, I;Weissman, IL;Minary, P;Majeti, R;Constantinescu, SN;Piehler, J;Garcia, KC; | Tuning cytokine receptor signaling by re-orienting dimer geometry with surrogate ligands | Cell | 25728669 | ficolled cells (32K) from a single healthy donor (donor ID #: 6024, lot#: BM4871) were purchased from Allcells, Alameda, CA 94502. Cells were subsequently treated with DNAase. Milteny CD34 magnetic beads were used to enrich for CD34+ cells with | Full Paper |
2015 | Vierstra, J;Reik, A;Chang, KH;Stehling-Sun, S;Zhou, Y;Hinkley, SJ;Paschon, DE;Zhang, L;Psatha, N;Bendana, YR;O'Neil, CM;Song, AH;Mich, AK;Liu, PQ;Lee, G;Bauer, DE;Holmes, MC;Orkin, SH;Papayannopoulou, T;Stamatoyannopoulos, G;Rebar, EJ;Gregory, PD;Urnov, FD;Stamatoyannopoulos, JA; | Functional footprinting of regulatory DNA | Nature methods | 26322838 | Human mobilized CD34 + cells (adult) were purchased from AllCells. Small-scale mRNA transfections were performed with a BTX device (Harvard Apparatus), using the CD34 + cell Save | Full Paper |
2015 | Bunin, A;Sisirak, V;Ghosh, HS;Grajkowska, LT;Hou, ZE;Miron, M;Yang, C;Ceribelli, M;Uetani, N;Chaperot, L;Plumas, J;Hendriks, W;Tremblay, ML;Häcker, H;Staudt, LM;Green, PH;Bhagat, G;Reizis, B; | Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic Cells | Immunity | 26231120 | PBMC were isolated from healthy adult volunteers by Histopaque density gradient centrifugation; where indicated, pDCs were enriched using the Diamond pDC isolation kit (Miltenyi Biotec) to 95% purity. Human PBMC subsets for expression analysis were p | Full Paper |
2015 | Behbehani, GK;Samusik, N;Bjornson, ZB;Fantl, WJ;Medeiros, BC;Nolan, GP; | Mass Cytometric Functional Profiling of Acute Myeloid Leukemia Defines Cell-Cycle and Immunophenotypic Properties That Correlate with Known Responses to Therapy | Cancer discovery | 26091827 | Healthy control samples were obtained from Allcells using the same protocol. The exception was healthy donor sample #1; this sample was incubated for approximately 1 hour with IdU Save | Full Paper |
2015 | Casero, D;Sandoval, S;Seet, CS;Scholes, J;Zhu, Y;Ha, VL;Luong, A;Parekh, C;Crooks, GM; | Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T cell lineages | Nature immunology | 26502406 | Anonymous normal human thymi were obtained via the UCLA Translational Pathology Core Laboratory and the CHLA cardiovascular thoracic surgery department. Bone marrow (BM) was obtained from donors via ALLCELLS, Alameda, CA. All tissues were collected a | Full Paper |
2015 | Swaminathan, S;Klemm, L;Park, E;Papaemmanuil, E;Ford, A;Kweon, SM;Trageser, D;Hasselfeld, B;Henke, N;Mooster, J;Geng, H;Schwarz, K;Kogan, SC;Casellas, R;Schatz, DG;Lieber, MR;Greaves, MF;Müschen, M; | Mechanisms of clonal evolution in childhood acute lymphoblastic leukemia | Nature immunology | 25985233 | Human CD19 + cord blood B cells were obtained from AllCells. AllCells procures cord blood subsequent to receiving informed consent from its donors. We have approval to use cord Save | Full Paper |
2015 | Pandolfi, A;Stanley, RF;Yu, Y;Bartholdy, B;Pendurti, G;Gritsman, K;Boultwood, J;Chernoff, J;Verma, A;Steidl, U; | PAK1 is a therapeutic target in acute myeloid leukemia and myelodysplastic syndrome | Blood | 26170031 | Poor clinical outcome of acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) has been attributed to failure of current chemotherapeutic regimens to target leukemic Save | Full Paper |
2015 | Patel, V;Balakrishnan, K;Keating, MJ;Wierda, WG;Gandhi, V; | Expression of executioner procaspases and their activation by a procaspase-activating compound in chronic lymphocytic leukemia cells | Blood | 25538042 | Normal B lymphocytes (freshly isolated negatively selected CD19 + B cells) were purchased from AllCells, and were kept in RPMI-1640 medium with 10% fetal bovine serum (FBS). Save | Full Paper |
2015 | Iwasa, M;Miura, Y;Fujishiro, A;Fujii, S;Sugino, N;Yoshioka, S;Tamura, A;Sato, A;Yokota, A;Kito, K;Ando, A;Hirai, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T; | Bortezomib Attenuates Adhesion of B Cell Precursor Acute Lymphoblastic Lleukemia Cells to Bone Marrow Mesenchymal Stromal/Stem Cells Via Regulating SPARC Expression | Blood | We isolated human BM-MSCs from normal bone marrow samples (AllCells, Emeryville, CA). When Nalm6 cells were co-cultured with human BM-MSCs, they were prone to adhere to BM Save;The prognosis of adults with B cell precursor acute lymphoblastic leukem | Full Paper | |
2015 | Shenoy, S;Eapen, M;Wu, J;Walters, M;Levine, J;Logan, B;Gersten, I;Kamani, N; | A Multicenter Phase II Trial of Unrelated Donor Reduced Intensity Bone Marrow Transplantation for Children with Severe Sickle Cell Disease (SCURT): Results of the Blood and Marrow Transplant Clinical Trials Network (BMT CTN 0601) Study | Blood | Background: Children with severe sickle cell disease (SCD) experience organ damage, poor quality of life, and a risk of premature mortality. Allogeneic bone marrow transplantation (BMT) halts disease progression when stable donor eryth | Full Paper | |
2015 | Krishnamurti, L;Sullivan, K;Kamani, N;Waller, E;Abraham, A;Campigotto, F;Zhang, W;Smith, S;Hassell, K;Decastro, L;Wu, C;Neuberg, D;Walters, M; | Results of a Multicenter Pilot Investigation of Bone Marrow Transplantation in Adults with Sickle Cell Disease (STRIDE) | Blood | Background: Hematopoietic cell transplantation (HCT) for sickle cell disease (SCD) is potentially curative, yet applied sparingly in adults due in part to limited donor availability and to concerns about safety. We conducted a multicenter pilot clini | Full Paper | |
2015 | Cappelli, B;Bernaudin, F;Ruggeri, A;Labopin, M;Volt, F;Simoes, B;Ferster, A;Dupont, S;de la Fuente, J;Dalle, J;Zecca, M;Robin, M;Walters, M;Dhedin, N;Michel, G;Lutz, P;Neven, B;Bertrand, Y;Vannier, J;Ayas, M;Matthes, S;Elayoubi, H;Devergie, A;Kenzey, C;Locatelli, F;Peters, C;Rocha, V;Eapen, M;Gluckman, E; | Hematopoietic Stem Cell Transplantation from HLA Identical Sibling Forsickle Cell Disease an International Survey on Behalf of Eurocord-Monacord, EBMT Paediatric Disease Working Party and CIBMTR | Blood | Introduction: Hematopoietic stem cell transplantation (HSCT) is, to date, the only curative therapy for sickle cell disease (SCD). However, HSCT is offered to relatively few patients with SCD for a number of reasons including lack of a | Full Paper | |
2015 | Kanter, J;Walters, M;Hsieh, M;Thompson, A;Krishnamurti, L;Kwiatkowski, J;Kamble, R;von Kalle, C;Kuypers, F;Cavazzana, M;Leboulch, P;Sandler, L;Soni, S;Tisdale, J; | Initial Results from Study Hgb-206: A Phase 1 Study Evaluating Gene Therapy By Transplantation of Autologous CD34+ Stem Cells Transduced Ex Vivo with the Lentiglobin BB305 Lentiviral Vector in Subjects with Severe Sickle Cell Disease | Blood | Background: Hematopoietic stem cell (HSC) gene transfer has the potential to reduce or eliminate the symptoms of severe sickle cell disease (SCD). Previously reported early results in a single subject with severe SCD from the ongoing study HGB-205 su | Full Paper | |
2015 | Walters, M;Rasko, J;Hongeng, S;Kwiatkowski, J;Schiller, G;Kletzel, M;Ho, P;Vichinsky, E;von Kalle, C;Cavazzana, M;Leboulch, P;Petrusich, A;Soni, S;Thompson, A; | Update of Results from the Northstar Study (HGB-204): A Phase 1/2 Study of Gene Therapy for Beta-Thalassemia Major Via Transplantation of Autologous Hematopoietic Stem Cells Transduced Ex-Vivo with a Lentiviral Beta AT87Q-Globin Vector (LentiGlobin BB305 Drug Product) | Blood | Background: Hematopoietic stem cell (HSC) gene transfer has the potential to induce globin production and mitigate or eliminate blood transfusions in patients with β-thalassemia major. Previously reported early results in subjects with β-thalassemia | Full Paper | |
2015 | Fujishiro, A;Miura, Y;Iwasa, M;Fujii, S;Tamura, A;Sato, A;Yokota, A;Sugino, N;Hirai, H;Ando, A;Ichinohe, T;Maekawa, T; | Vitamin K2 Supports Hematopoiesis through Acting on Bone Marrow Mesenchymal Stromal/Stem Cells | Blood | [Methods] Normal bone marrow (BM) samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA). BM-CD34 + cells were isolated from BM-mononuclear cells Save;[Background] Myelodysplastic syndrome is an intractable disorder cha | Full Paper | |
2015 | Kikushige, Y;Miyamoto, T;Yuda, J;Jabbarzadeh-Tabrizi, S;Shima, T;Takayanagi, S;Niiro, H;Yurino, A;Miyawaki, K;Takenaka, K;Iwasaki, H;Akashi, K; | A TIM-3/Gal-9 Autocrine Stimulatory Loop Drives Self-Renewal of Human Myeloid Leukemia Stem Cells and Leukemic Progression | Cell stem cell | 26279267 | summarizes the primary AML patients’ characteristics analyzed in this study. Human adult BM and PB samples were obtained from healthy donors or purchased from AllCells. CB cells were obtained from full-term deliveries (provided by the Kyushu Block Re | Full Paper |
2015 | Jeong, JY;Levine, MS;Abayasekara, N;Berliner, N;Laubach, J;Vanasse, GJ; | The non-peptide thrombopoietin receptor agonist eltrombopag stimulates megakaryopoiesis in bone marrow cells from patients with relapsed multiple myeloma | Journal of hematology & oncology | 25886818 | were also obtained from commercial sources (AllCells, Emeryville, CA and Proteogenix, from Lonza (Basel, Switzerland) and AllCells, respectively. Normal human platelets were Save | Full Paper |
2015 | Goldstein, RL;Yang, SN;Taldone, T;Chang, B;Gerecitano, J;Elenitoba-Johnson, K;Shaknovich, R;Tam, W;Leonard, JP;Chiosis, G;Cerchietti, L;Melnick, A; | Pharmacoproteomics identifies combinatorial therapy targets for diffuse large B cell lymphoma | The Journal of clinical investigation | 26529251 | HEPES, and penicillin/streptomycin. CD19+ cells were purchased from AllCells. PU-H71 was synthesized as previously reported (5). Ibrutinib was provided by Pharmacyclics. R406, BKM120, and sotrastaurin were purchased from Selleck Chemicals. MI-2 was p | Full Paper |
2015 | Ma, SD;Xu, X;Plowshay, J;Ranheim, EA;Burlingham, WJ;Jensen, JL;Asimakopoulos, F;Tang, W;Gulley, ML;Cesarman, E;Gumperz, JE;Kenney, SC; | LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesis | The Journal of clinical investigation | 25485679 | Alternatively, commercially purchased CD34-depleted human cord blood mononuclear cells (AllCells LLC, catalog no. CB117) were mock-infected or infected with WT EBV or LMP1-KO EBV in vitro for 1.5 hours, after which 12 × 106-25 × 106 cells were inject | Full Paper |
2015 | Chichili, GR;Huang, L;Li, H;Burke, S;He, L;Tang, Q;Jin, L;Gorlatov, S;Ciccarone, V;Chen, F;Koenig, S;Shannon, M;Alderson, R;Moore, PA;Johnson, S;Bonvini, E; | A CD3xCD123 bispecific DART for redirecting host T cells to myelogenous leukemia: preclinical activity and safety in nonhuman primates | Science translational medicine | 26019218 | Cryopreserved AML patient primary PBMCs and bone marrow mononuclear cells (BMMNCs) were purchased from AllCells, LLC, which obtains biological specimens from a donor Save | Full Paper |
2015 | Lee, ST;Muench, MO;Fomin, ME;Xiao, J;Zhou, M;de Smith, A;Martín-Subero, JI;Heath, S;Houseman, EA;Roy, R;Wrensch, M;Wiencke, J;Metayer, C;Wiemels, JL; | Epigenetic remodeling in B-cell acute lymphoblastic leukemia occurs in two tracks and employs embryonic stem cell-like signatures | Nucleic acids research | 25690899 | l, pre-B-II cells for whole genome bisulfite sequencing (WGBS) were purified. Purified normal mature B-cells, T-cells, regulatory T-cells, NK-cells, monocytes and granulocytes were purchased from the Allcells Corp. DNAs were extracted as described ab | Full Paper |
2015 | Jung, N;Dai, B;Gentles, AJ;Majeti, R;Feinberg, AP; | An LSC epigenetic signature is largely mutation independent and implicates the HOXA cluster in AML pathogenesis | Nature communications | 26444494 | n cancer biology using large data sets. Methods Human samples Fresh human bone marrow mononuclear cells (BMMCs) from healthy donors (2 × 108 cells per donor, Catalogue#: ABM006) were purchased from ALLCELLS (Emeryville, CA). A CD34+ cell-enrichment | Full Paper |
2015 | Naka, K;Jomen, Y;Ishihara, K;Kim, J;Ishimoto, T;Bae, EJ;Mohney, RP;Stirdivant, SM;Oshima, H;Oshima, M;Kim, DW;Nakauchi, H;Takihara, Y;Kato, Y;Ooshima, A;Kim, SJ; | Dipeptide species regulate p38MAPK-Smad3 signalling to maintain chronic myelogenous leukaemia stem cells | Nature communications | 26289811 | Viable BM MNCs from three human patients with chronic phase CML were purchased from Allcells (#06-255, #06-620 and #147742, Alameda, CA, USA). We stained these cells with Save | Full Paper |
2015 | Buchner, M;Park, E;Geng, H;Klemm, L;Flach, J;Passegué, E;Schjerven, H;Melnick, A;Paietta, E;Kopanja, D;Raychaudhuri, P;Müschen, M; | Identification of FOXM1 as a therapeutic target in B-cell lineage acute lymphoblastic leukaemia | Nature communications | 25753524 | nslocation of oestrogen receptor fusion proteins, 4-OHT was added at a concentration of 500 nM. Cell sorting For magnetic bead sorting, peripheral blood or BM mononuclear cells were purchased from AllCells LLC and CD19-enrichment was performed by ma | Full Paper |
2015 | Zhang, W;Wray, LS;Rnjak-Kovacina, J;Xu, L;Zou, D;Wang, S;Zhang, M;Dong, J;Li, G;Kaplan, DL;Jiang, X; | Vascularization of hollow channel-modified porous silk scaffolds with endothelial cells for tissue regeneration | Biomaterials | 25934280 | HUVECs were purchased from AllCells biology technology limited company (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth Save | Full Paper |
2015 | Coquery, CM;Loo, WM;Wade, NS;Bederman, AG;Tung, KS;Lewis, JE;Hess, H;Erickson, LD; | BAFF regulates follicular helper t cells and affects their accumulation and interferon-γ production in autoimmunity | Arthritis & rheumatology (Hoboken, N.J.) | 25385309 | Additional blood from SLE patients was purchased from AllCells to increase the range of SLE Disease Activity Index (SLEDAI) scores 24. AllCells used the same protocol and materials Save | Full Paper |
2015 | Stephenson, ST;Brown, LA;Helms, MN;Qu, H;Brown, SD;Brown, MR;Fitzpatrick, AM; | Cysteine oxidation impairs systemic glucocorticoid responsiveness in children with difficult-to-treat asthma | The Journal of allergy and clinical immunology | 25748343 | Cell culture experiments were performed with THP-1 monocytes (ATCC®, Manassas, VA), MM.1S B lymphoblasts (ATCC®) and primary PBMCs from healthy donors (AllCells, Alameda, CA). Reduced and oxidized conditions were created by adding 300 μL of 10 mM cys | Full Paper |
2015 | Karjalainen, K;Jaalouk, DE;Bueso-Ramos, C;Bover, L;Sun, Y;Kuniyasu, A;Driessen, WH;Cardó-Vila, M;Rietz, C;Zurita, AJ;O'Brien, S;Kantarjian, HM;Cortes, JE;Calin, GA;Koivunen, E;Arap, W;Pasqualini, R; | Targeting IL11 Receptor in Leukemia and Lymphoma: A Functional Ligand-Directed Study and Hematopathology Analysis of Patient-Derived Specimens | Clinical cancer research : an official journal of the American Association for Cancer Research | 25779950 | Normal blood and bone marrow samples were commercially obtained (AllCells). Cells were maintained in humidified hypoxia chambers (HeraCell 150; Thermo Electron Corporation) Save | Full Paper |
2015 | Morelli, E;Leone, E;Cantafio, ME;Di Martino, MT;Amodio, N;Biamonte, L;Gullà, A;Foresta, U;Pitari, MR;Botta, C;Rossi, M;Neri, A;Munshi, NC;Anderson, KC;Tagliaferri, P;Tassone, P; | Selective targeting of IRF4 by synthetic microRNA-125b-5p mimics induces anti-multiple myeloma activity in vitro and in vivo | Leukemia | 25987254 | Biosciences, CA, USA); lentiviral particles were produced and transduced as previously described.19 RNA extraction and qRT-PCR. RNA samples of healthy donors BM-derived plasma cells were purchased (AllCells LLC, Alameda, CA, USA). Total RNA extracti | Full Paper |
2015 | Holm, F;Hellqvist, E;Mason, CN;Ali, SA;Delos-Santos, N;Barrett, CL;Chun, HJ;Minden, MD;Moore, RA;Marra, MA;Runza, V;Frazer, KA;Sadarangani, A;Jamieson, CH; | Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal | Proceedings of the National Academy of Sciences of the United States of America | 26621726 | from patients at the University of California, San Diego and the University of Toronto Health Network according to Institutional Review Board-approved informed consent protocols. Human CD34+ cord blood cells and NPB cells were purchased from AllCells | Full Paper |
2015 | Sundaravel, S;Duggan, R;Bhagat, T;Ebenezer, DL;Liu, H;Yu, Y;Bartenstein, M;Unnikrishnan, M;Karmakar, S;Liu, TC;Torregroza, I;Quenon, T;Anastasi, J;McGraw, KL;Pellagatti, A;Boultwood, J;Yajnik, V;Artz, A;Le Beau, MM;Steidl, U;List, AF;Evans, T;Verma, A;Wickrema, A; | Reduced DOCK4 expression leads to erythroid dysplasia in myelodysplastic syndromes | Proceedings of the National Academy of Sciences of the United States of America | 26578796 | CD34 + HSCs and progenitor cells were purified from G-mobilized peripheral blood of healthy donors purchased from AllCells, Inc. using a CliniMACS (Miltenyi Biotec, Inc.) instrument. Save | Full Paper |
2015 | Yea, K;Zhang, H;Xie, J;Jones, TM;Lin, CW;Francesconi, W;Berton, F;Fallahi, M;Sauer, K;Lerner, RA; | Agonist antibody that induces human malignant cells to kill one another | Proceedings of the National Academy of Sciences of the United States of America | 26487683 | The one patient with M5 differentiated cells had been treated with Ara-C for 4 mo before collection of cells; the other six patients were untreated. Samples of leukemic cells obtained by BM aspiration or from PB were purchased from the AllCells corpo | Full Paper |
2015 | Lovat, F;Fassan, M;Gasparini, P;Rizzotto, L;Cascione, L;Pizzi, M;Vicentini, C;Balatti, V;Palmieri, D;Costinean, S;Croce, CM; | miR-15b/16-2 deletion promotes B-cell malignancies | Proceedings of the National Academy of Sciences of the United States of America | 26324892 | Technologies) according to the manufacturer’s protocol. Real-time PCR experiments were carried out using miR-15b and pri-miR-15b assays for real-time PCR (Applied Biosystems) according to the manufacturer’s protocol. Control human cord blood CD19+ B | Full Paper |
2015 | Mori, Y;Chen, JY;Pluvinage, JV;Seita, J;Weissman, IL; | Prospective isolation of human erythroid lineage-committed progenitors | Proceedings of the National Academy of Sciences of the United States of America | 26195758 | Antibodies, Cell Staining, and Sorting. The sorting procedures for HSCs and myeloid progenitor populations that we previously reported (20) were slightly modified. In brief, bone marrow mononuclear cells, purchased from AllCells, were first stained w | Full Paper |
2015 | Li, Y;Rogoff, HA;Keates, S;Gao, Y;Murikipudi, S;Mikule, K;Leggett, D;Li, W;Pardee, AB;Li, CJ; | Suppression of cancer relapse and metastasis by inhibiting cancer stemness | Proceedings of the National Academy of Sciences of the United States of America | 25605917 | Human CD34 + bone marrow cells (AllCells) were cultured in MethoCult media to form colonies as described by the manufacturer (Stemcell Technologies). BBI608 (2-Acetyl-4H,9H- Save | Full Paper |
2015 | Kuramitsu, M;Okuma, K;Yamochi, T;Sato, T;Sasaki, D;Hasegawa, H;Umeki, K;Kubota, R;Sobata, R;Matsumoto, C;Kaneko, N;Naruse, I;Yamagishi, M;Nakashima, M;Momose, H;Araki, K;Mizukami, T;Mizusawa, S;Okada, Y;Ochiai, M;Utsunomiya, A;Koh, KR;Ogata, M;Nosaka, K;Uchimaru, K;Iwanaga, M;Sagara, Y;Yamano, Y;Satake, M;Okayama, A;Mochizuki, M;Izumo, S;Saito, S;Itabashi, K;Kamihira, S;Yamaguchi, K;Watanabe, T;Hamaguchi, I; | Standardization of Quantitative PCR for Human T-Cell Leukemia Virus Type 1 in Japan: a Collaborative Study | Journal of clinical microbiology | 26292315 | PBMCs were purchased from AllCells Inc. (Alameda, CA, USA). TL-Om1 cells were in RPMI 1640 supplemented with 10% FBS at 37C according to the protocol provided by AllCells Inc. Save | Full Paper |
2015 | Kuramitsu, M;Okuma, K;Yamagishi, M;Yamochi, T;Firouzi, S;Momose, H;Mizukami, T;Takizawa, K;Araki, K;Sugamura, K;Yamaguchi, K;Watanabe, T;Hamaguchi, I; | Identification of TL-Om1, an adult T-cell leukemia (ATL) cell line, as reference material for quantitative PCR for human T-lymphotropic virus 1 | Journal of clinical microbiology | 25502533 | PBMCs were kindly provided by the Japanese Red Cross or purchased from AllCells (Alameda, CA, USA). TL-Om1 cells, an ATL-derived cell line established by Sugamura et al. (17), were maintained in RPMI 1640 (Sigma, St. Louis, MO, USA) containing 10% fe | Full Paper |
2015 | Lancet, JE;Roboz, GJ;Cripe, LD;Michelson, GC;Fox, JA;Leavitt, RD;Chen, T;Hawtin, R;Craig, AR;Ravandi, F;Maris, MB;Stuart, RK;Karp, JE; | A phase 1b/2 study of vosaroxin in combination with cytarabine in patients with relapsed or refractory acute myeloid leukemia | Haematologica | 25381131 | clinical samples, induction of pDNA-PKcs following a 2 h exposure to vosaroxin was examined. PBMC from an AML patient (80% blasts; obtained from AllCells LLC) were treated ex vivo with a dose titration of vosaroxin or vehicle for 2 h. Samples were an | Full Paper |
2015 | Sanyal, M;Morimoto, M;Baradaran-Heravi, A;Choi, K;Kambham, N;Jensen, K;Dutt, S;Dionis-Petersen, KY;Liu, LX;Felix, K;Mayfield, C;Dekel, B;Bokenkamp, A;Fryssira, H;Guillen-Navarro, E;Lama, G;Brugnara, M;Lücke, T;Olney, AH;Hunley, TE;Polat, AI;Yis, U;Bogdanovic, R;Mitrovic, K;Berry, S;Najera, L;Najafian, B;Gentile, M;Nur Semerci, C;Tsimaratos, M;Lewis, DB;Boerkoel, CF; | Lack of IL7Rα expression in T cells is a hallmark of T-cell immunodeficiency in Schimke immuno-osseous dysplasia (SIOD) | Clinical immunology (Orlando, Fla.) | 26499378 | The protocol and informed consent procedures were provided according to the Declaration of Helsinki guidelines. The clinical data for patients were obtained from questionnaires completed by the attending physician as well as from the medical records. | Full Paper |
2015 | Liu, J;Gaj, T;Wallen, MC;Barbas, CF; | Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineering | Molecular therapy. Nucleic acids | 25756962 | T-activator Dynabeads (Dynal/Life Technologies), and 50 U/ml recombinant interleukin-2 (rIL-2; R&D Systems). Bone marrow-derived CD34+ hematopoietic stem/progenitor cells (HSPCs) were obtained from AllCells, LLC at a purity of >97% (Supplementary Fig | Full Paper |
2015 | Kim, SY;Theunissen, JW;Balibalos, J;Liao-Chan, S;Babcock, MC;Wong, T;Cairns, B;Gonzalez, D;van der Horst, EH;Perez, M;Levashova, Z;Chinn, L;D'Alessio, JA;Flory, M;Bermudez, A;Jackson, DY;Ha, E;Monteon, J;Bruhns, MF;Chen, G;Migone, TS; | A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies | Blood cancer journal | 26024286 | Additional frozen AML and CLL patient specimens for flow analysis were from AllCells and the University of California San Diego, respectively. Primary solid tumors and normal Save | Full Paper |
2015 | Crews, LA;Jiang, Q;Zipeto, MA;Lazzari, E;Court, AC;Ali, S;Barrett, CL;Frazer, KA;Jamieson, CH; | An RNA editing fingerprint of cancer stem cell reprogramming | Journal of translational medicine | 25889244 | Peripheral blood or bone marrow samples were processed by Ficoll density centrifugation and viable cells stored in liquid nitrogen. Normal peripheral blood mononuclear cells (MNC) were obtained from AllCells (Alameda, CA). Mononuclear cells from cont | Full Paper |
2015 | Sloan, DD;Lam, CY;Irrinki, A;Liu, L;Tsai, A;Pace, CS;Kaur, J;Murry, JP;Balakrishnan, M;Moore, PA;Johnson, S;Nordstrom, JL;Cihlar, T;Koenig, S; | Targeting HIV Reservoir in Infected CD4 T Cells by Dual-Affinity Re-targeting Molecules (DARTs) that Bind HIV Envelope and Recruit Cytotoxic T Cells | PLoS pathogens | 26539983 | pended with FACS buffer, and analyzed by flow cytometry using a FACSCalibur (BD Biosciences) and FlowJo software (TreeStar). In Vitro HIV-Infected Cell Killing Assay PBMCs from healthy participants (AllCells) were isolated by Ficoll-plaque gradient. | Full Paper |
2015 | Ma, L;Aijima, R;Hoshino, Y;Yamaza, H;Tomoda, E;Tanaka, Y;Sonoda, S;Song, G;Zhao, W;Nonaka, K;Shi, S;Yamaza, T; | Transplantation of mesenchymal stem cells ameliorates secondary osteoporosis through interleukin-17-impaired functions of recipient bone marrow mesenchymal stem cells in MRL/lpr mice | Stem cell research & therapy | 26012584 | University (protocol number: A21-044-1). Human MSC isolation and culture Human MSCs were isolated based on the adherent colony-forming unit fibroblasts (CFU-F) method [20]. Bone marrow cells (BMCs) (AllCells, Berkeley, CA, USA) were seeded at 1×107 | Full Paper |
2015 | Di Lullo, G;Marcatti, M;Heltai, S;Brunetto, E;Tresoldi, C;Bondanza, A;Bonini, C;Ponzoni, M;Tonon, G;Ciceri, F;Bordignon, C;Protti, MP; | Th22 cells increase in poor prognosis multiple myeloma and promote tumor cell growth and survival | Oncoimmunology | 26155400 | had approved the study protocol and written informed consent was obtained from all donors. Blood samples of 15 healthy donors from the Institutional Blood Bank and BM aspirates from 4 healthy donors (AllCells, Life Science Division) were used as cont | Full Paper |
2015 | Alva, A;Friedlander, T;Clark, M;Huebner, T;Daignault, S;Hussain, M;Lee, C;Hafez, K;Hollenbeck, B;Weizer, A;Premasekharan, G;Tran, T;Fu, C;Ionescu-Zanetti, C;Schwartz, M;Fan, A;Paris, P; | Circulating Tumor Cells as Potential Biomarkers in Bladder Cancer | The Journal of urology | 25912492 | candidacy for cisplatin based chemotherapy and 4) candidacy for radical cystectomy with curative intent. Two patients were lost to followup. Of the patients 16 underwent pathological staging at cystectomy. A second cohort of 11 patients with metastas | Full Paper |
2015 | Yang, J;Khan, M;Zhang, L;Ren, X;Guo, J;Feng, Y;Wei, S;Zhang, W; | Antimicrobial surfaces grafted random copolymers with REDV peptide beneficial for endothelialization | Journal of materials chemistry. B | 32264578 | Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells Biomart (Shanghai) and cultured in high glucose DMEM supplemented with 10% FBS in an incubator ( Save | Full Paper |
2015 | Fernandes, S;Chong, JJH;Paige, SL;Iwata, M;Torok-Storb, B;Keller, G;Reinecke, H;Murry, CE; | Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac Repair | Stem cell reports | 26607951 | vol/vol), supplemented with L-glutamine, ascorbic acid, transferrin, monothioglycerol, cyclosporine A (200 nM, Wako), and Y-27632 (10 μM). Fresh bone marrow mononuclear cells were purchased from AllCells LLC (ABM024). After overnight shipment, they | Full Paper |
2015 | McIntosh, BE;Brown, ME;Duffin, BM;Maufort, JP;Vereide, DT;Slukvin, II;Thomson, JA; | Nonirradiated NOD,B6.SCID Il2rγ-/- Kit(W41/W41) (NBSGW) mice support multilineage engraftment of human hematopoietic cells | Stem cell reports | 25601207 | cedures for oligos and allelic determination. The University of Wisconsin Medical School’s Animal Care and Use Committee authorized all experiments. Human Cell Engraftment Frozen human CD34+ CBCs (AllCells) were thawed per manufacturer’s recommendat | Full Paper |
2015 | Tang, W;Song, H;Cai, W;Shen, X; | Real Time Monitoring of Inhibition of Adipogenesis and Angiogenesis by (-)-Epigallocatechin-3-Gallate in 3T3-L1 Adipocytes and Human Umbilical Vein Endothelial Cells | Nutrients | 26516907 | TGTCTTTA-3′ and reverse, 5′-AGCCCTCCACCTCCCTGTAG-3′; β-actin forward, 5′-CCTCTATGCCAACACAGT-3′ and reverse, 5′-AGCCACCAATCCACACAG 3′. 2.8. Co-Culture of HUVEC and 3T3-L1 Cells HUVECs (ALLCELLS, Shanghai, China) were seeded (6000 cells/well) into E-p | Full Paper |
2015 | Griffin, DO;Goff, SP; | HIV-1 Is Restricted prior to Integration of Viral DNA in Primary Cord-Derived Human CD34+ Cells | Journal of virology | 25995256 | CD34 cells were isolated with magnetic beads from cord blood samples obtained from the Long Island Blood Service or obtained preisolated from AllCells (11, 12). CD34 cells were put Save | Full Paper |
2015 | Wu, X;Sereno, AJ;Huang, F;Lewis, SM;Lieu, RL;Weldon, C;Torres, C;Fine, C;Batt, MA;Fitchett, JR;Glasebrook, AL;Kuhlman, B;Demarest, SJ; | Fab-based bispecific antibody formats with robust biophysical properties and biological activity | mAbs | 25774965 | Untreated, purified T cells from a human donor were purchased from ALLCELLS (Cat#PB009-1F). The cells were thawed and washed in 10% FBS (heat inactivated, Corning) plus 50 Save | Full Paper |
2015 | Li, X;Tian, F;Wang, F; | The effects of black soybean (Glycine max var.) on chronic cervical pain of sedentary office workers in a northern Chinese population | Food & function | 26295483 | 5.0 × 105 cells per ml was placed into each well of a 96-well plate (ALLCELLS, Shanghai, China). After 24 h, cells were incubated with 1 Ci per well of [3H]thymidine (67 Ci mmol−1) for five h, and then collected using a Brandell 96-sample harvester ( | Full Paper |
2015 | Mata, MF;Lopes, JP;Ishikawa, M;Alaiti, MA;Cabral, JM;da Silva, CL;Costa, MA; | Scaling up the ex vivo expansion of human circulating CD34(+)progenitor cells with upregulation of angiogenic and anti-inflammatory potential | Cytotherapy | 26475753 | CD34 + cells isolated from mobilized peripheral blood of healthy donors (n = 5) were obtained from AllCells and were cultured for 7 days in 30-mL cell culture bags (VueLife FEP Bag, Save | Full Paper |
2015 | Ma, Y;Reilly, S;Fischer, S;Henshaw, M;Pierce, J;Poudel, A;Reems, J; | Human platelet lysate produced at industrial scale for use as fetal bovine serum replacement in cell manufacturing protocols | Cytotherapy | Tissue Origin: 9 different donors of fresh human bone marrow (hBM) from the posterior iliac crest were obtained from Lonza or AllCells. 6 different donors of fresh human lipoaspirate Save | Full Paper | |
2015 | Fierro, FA;O'Neal, AJ;Beegle, JR;Chávez, MN;Peavy, TR;Isseroff, RR;Egaña, JT; | Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cells | Frontiers in cell and developmental biology | 26579521 | r bone marrow samples of healthy human donors who gave informed consent to the research protocols (approved by the institutional review board of the Technical University of Dresden) or purchased from Allcells (Alameda, CA). Mononuclear cell (MNC) fra | Full Paper |
2015 | Haqqani, AA;Marek, SL;Kumar, J;Davenport, M;Wang, H;Tilton, JC; | Central memory CD4+ T cells are preferential targets of double infection by HIV-1 | Virology journal | 26559763 | Normal donor samples were de-identified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. A 10 year-old male with the Save | Full Paper |
2015 | Huang, H;Saravia, J;You, D;Shaw, AJ;Cormier, SA; | Impaired gamma delta T cell-derived IL-17A and inflammasome activation during early respiratory syncytial virus infection in infants | Immunology and cell biology | 25267484 | tes 2 hr prior to RSV infection. Sample isolation purity and adoptive transfer efficiency were verified by flow cytometry. CBMC/PBMC RIG-I activation Purified CBMCs and PBMCs from individual donors (AllCells.com) were thawed and counted according to | Full Paper |
2015 | Lynch, PJ;Thompson, EE;McGinnis, K;Rovira Gonzalez, YI;Lo Surdo, J;Bauer, SR;Hursh, DA; | Chromatin Changes at the PPAR-γ2 Promoter During Bone Marrow-Derived Multipotent Stromal Cell Culture Correlate With Loss of Gene Activation Potential | Stem cells (Dayton, Ohio) | 25640287 | BM-MSCs from six donors were purchased from AllCells (Alameda, CA, http://www.allcells.com/) and Lonza (Allendale, NJ, http://www.lonza.com/) (Table 1). All BM-MSCs used here were previously expanded, characterized, and cryopreserved for experimentat | Full Paper |
2015 | Münchow, EA;Albuquerque, MT;Zero, B;Kamocki, K;Piva, E;Gregory, RL;Bottino, MC; | Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration | Dental materials : official publication of the Academy of Dental Materials | 26116414 | The guidelines provided by the International Standards Organization/ISO [35] were followed in order to detect toxicity levels of the electrospun membranes [34]. Human dental pulp stem cells (hDPSCs, AllCells LLC., Alameda, CA, USA) obtained from perm | Full Paper |
2015 | Babad, J;Mukherjee, G;Follenzi, A;Ali, R;Roep, BO;Shultz, LD;Santamaria, P;Yang, OO;Goldstein, H;Greiner, DL;DiLorenzo, TP; | Generation of β cell-specific human cytotoxic T cells by lentiviral transduction and their survival in immunodeficient human leucocyte antigen-transgenic mice | Clinical and experimental immunology | 25302633 | Several β cell antigens recognized by T cells in the non-obese diabetic (NOD) mouse model of type 1 diabetes (T1D) are also T cell targets in the human disease. While numerous Save | Full Paper |
2015 | Liu, A;Wang, Y;Ding, Y;Baez, I;Payne, KJ;Borghesi, L; | Cutting Edge: Hematopoietic Stem Cell Expansion and Common Lymphoid Progenitor Depletion Require Hematopoietic-Derived, Cell-Autonomous TLR4 in a Model of Chronic Endotoxin | Journal of immunology (Baltimore, Md. : 1950) | 26276875 | De-identified cord blood cells were obtained with approval from the University of Pittsburgh School of Medicine Institutional Review Board. Purified CD34+ cord blood cells were purchased from AllCells, or cord blood obtained from Loma Linda Universit | Full Paper |
2015 | Katano, I;Takahashi, T;Ito, R;Kamisako, T;Mizusawa, T;Ka, Y;Ogura, T;Suemizu, H;Kawakami, Y;Ito, M; | Predominant development of mature and functional human NK cells in a novel human IL-2-producing transgenic NOG mouse | Journal of immunology (Baltimore, Md. : 1950) | 25712215 | Germany) in combination with biotin-conjugated anti-mouse CD45 mAb (BD Biosciences), all cells, with the exception of human CD56+ NK cells, were magnetically labeled and eliminated using a MACS LD column (Miltenyi Biotec). The purity of the isolated | Full Paper |
2015 | Montezuma-Rusca, JM;Moir, S;Kardava, L;Buckner, CM;Louie, A;Kim, LJ;Santich, BH;Wang, W;Fankuchen, OR;Diaz, G;Daub, JR;Rosenzweig, SD;Chun, TW;Li, Y;Braylan, RC;Calvo, KR;Fauci, AS; | Bone marrow plasma cells are a primary source of serum HIV-1-specific antibodies in chronically infected individuals | Journal of immunology (Baltimore, Md. : 1950) | 25681347 | obtained from posterior iliac crests under conscious sedation and local anesthesia. In addition, cryopreserved bone marrow mononuclear cells (BMMCs) were obtained from commercially available sources (Allcells or Stemcell Technologies) | Full Paper |
2015 | Kang, HS;Choi, SH;Kim, BS;Choi, JY;Park, GB;Kwon, TG;Chun, SY; | Advanced Properties of Urine Derived Stem Cells Compared to Adipose Tissue Derived Stem Cells in Terms of Cell Proliferation, Immune Modulation and Multi Differentiation | Journal of Korean medical science | 26713051 | or separated-(trans-well chamber, Corning, Corning, NY, USA) cultured with both stem cells; each well was composed with 1:100, 1:50, 1:25, or 1:12.5 stem cell to PBMC ratio. PBMCs were acquired from AllCells (Emeryville, CA, USA) and activated with 5 | Full Paper |
2015 | Wu, W;Zhang, X;Lv, H;Liao, Y;Zhang, W;Cheng, H;Deng, Z;Shen, J;Yuan, Q;Zhang, Y;Shen, W; | Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion | International journal of molecular medicine | 26260137 | cells (HUVECs) were purchased from AllCells (Shanghai, China) and cultured in 0.5% gelatin-coated dishes in endothelial cell growth medium (EGM; AllCells) according to the Save | Full Paper |
2015 | Kamocki, K;Nör, JE;Bottino, MC; | Dental pulp stem cell responses to novel antibiotic-containing scaffolds for regenerative endodontics | International endodontic journal | 25425048 | fetal bovine serum (FBS, Hyclone, Logan, UT, USA) and 1% penicillin- streptomycin (Sigma-Aldrich) in a humidified incubator at 37°C, with 5% CO2, was used to culture hDPSC (catalogue number - DP003F, AllCells LLC, Alameda, CA, USA) obtained from perm | Full Paper |
2015 | Press, T;Viale-Bouroncle, S;Felthaus, O;Gosau, M;Morsczeck, C; | EGR1 supports the osteogenic differentiation of dental stem cells | International endodontic journal | 24749562 | Dental follicle cells (DFCs), also called dental follicle stem cells (DFSCs), were purchased from AllCells© (Alameda, CA, USA). All cells were grown in standard basal medium, Dulbecco′s modified Eagle′s medium (DMEM; Sigma-Aldrich, Munich, Germany) s | Full Paper |
2015 | Lu, Q;Pandya, M;Rufaihah, AJ;Rosa, V;Tong, HJ;Seliktar, D;Toh, WS; | Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System | Stem cells international | 26124841 | used in this study were obtained commercially from AllCells, LLC. DPSCs were cultured in Dulbecco's modified eagle's medium (DMEM) (low glucose, Biowest, France) supplemented Save | Full Paper |
2015 | Chen, Q;Ye, W;Jian Tan, W;Mei Yong, KS;Liu, M;Qi Tan, S;Loh, E;Te Chang, K;Chye Tan, T;Preiser, PR;Chen, J; | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human | Scientific reports | 26456148 | ercoll. Contaminating red blood cells were lysed with ACK lysis buffer (Life Technologies). Isolation of CD56+ cells from MNCs was performed via cell sorting. Human adult BM MNCs were purchased from AllCells (Alameda, CA). To isolate MNCs from fetal | Full Paper |
2015 | Su, M;Hu, R;Jin, J;Yan, Y;Song, Y;Sullivan, R;Lai, L; | Efficient in vitro generation of functional thymic epithelial progenitors from human embryonic stem cells | Scientific reports | 26044259 | ed (2 Gy), transplanted under the kidney capsule with the solidified reaggregate of the hESC-derived cells, and injected i.v. with human bone marrow CD34+ cells (1 × 105 cells/mouse, obtained from AllCells, LLC, Alameda, CA). Twelve to 24 weeks after | Full Paper |
2015 | Nixon, CC;Schwartz, BH;Dixit, D;Zack, JA;Vatakis, DN; | Cocaine exposure impairs multilineage hematopoiesis of human hematopoietic progenitor cells mediated by the sigma-1 receptor [corrected] | Scientific reports | 25728014 | Mononuclear cells were obtained from the buffy coats of either processed fetal liver or cord blood or adult bone marrow (Allcells, Alameda, CA) following Ficoll-Hypaqe density gradient Save | Full Paper |
2015 | Feitosa, SA;Münchow, EA;Al-Zain, AO;Kamocki, K;Platt, JA;Bottino, MC; | Synthesis and characterization of novel halloysite-incorporated adhesive resins | Journal of dentistry | 26334950 | Next, human dental pulp stem cells (CD73, CD90, CD105, and CD166 positive) obtained from permanent third molars (hDPSCs, catalog number—DP003F, AllCells LLC, Alameda, CA, USA) [32] were cultured in DMEM supplemented with 10% FBS were seeded at the ce | Full Paper |
2015 | Lei, L;Wang, S;Wu, H;Ju, W;Peng, J;Qahtan, AS;Chen, C;Lu, Y;Peng, J;Zhang, X;Nie, H; | Optimization of release pattern of FGF-2 and BMP-2 for osteogenic differentiation of low-population density hMSCs | Journal of biomedical materials research. Part A | 24639043 | Fresh whole bone marrow was acquired from AllCells (Emeryville, CA). 0.5 mL of RosetteSep The hMSCs were isolated according to AllCells' protocol. Cells from passages 4-9 were Save | Full Paper |
2015 | Poleganov, MA;Eminli, S;Beissert, T;Herz, S;Moon, JI;Goldmann, J;Beyer, A;Heck, R;Burkhart, I;Barea Roldan, D;Türeci, Ö;Yi, K;Hamilton, B;Sahin, U; | Efficient Reprogramming of Human Fibroblasts and Blood-Derived Endothelial Progenitor Cells Using Nonmodified RNA for Reprogramming and Immune Evasion | Human gene therapy | 26381596 | Cord blood EPCs were derived from 5E7 frozen cord blood MNCs (AllCells). Both peripheral and cord blood MNCs were washed and cultured on rat collagen type I (BD Bioscience)- Save | Full Paper |
2015 | Suenaga, H;Furukawa, KS;Suzuki, Y;Takato, T;Ushida, T; | Bone regeneration in calvarial defects in a rat model by implantation of human bone marrow-derived mesenchymal stromal cell spheroids | Journal of materials science. Materials in medicine | 26449444 | p). After a recovery period of 8 weeks, bone regeneration at the defect sites was evaluated Isolation and culturing of human MSCs Fresh BM samples of 3-4 anonymous adult donors were obtained from AllCells (Berkeley, CA), and MSCs were isolated, as d | Full Paper |
2015 | Uchiyama, T;Kawabata, H;Miura, Y;Yoshioka, S;Iwasa, M;Yao, H;Sakamoto, S;Fujimoto, M;Haga, H;Kadowaki, N;Maekawa, T;Takaori-Kondo, A; | The role of growth differentiation factor 15 in the pathogenesis of primary myelofibrosis | Cancer medicine | 26276681 | Normal human BM mononuclear cells were separated from BM aspirates (AllCells) using Ficoll-Hypaque-Plus solution (GE Healthcare, Tokyo, Japan). CD34 + HPCs (Hematopoietic Save | Full Paper |
2015 | Wu, H;Fan, F;Liu, Z;Zhang, F;Liu, Y;Wei, Z;Shen, C;Cao, Y;Wang, A;Lu, Y; | The angiogenic responses induced by release of angiogenic proteins from tumor cell-activated platelets are regulated by distinct molecular pathways | IUBMB life | 26283102 | Gibco, Grand Island, NY), 100 U/mL penicillin and 100 μg/mL streptomycin, and grown in a 95% air and 5% CO2 humidified atmosphere at 37°C in Primary HUVECs were purchased from Allcells (Shanghai, China) and cultured in EBM media (Allcells) according | Full Paper |
2015 | Bam, R;Khan, S;Ling, W;Randal, SS;Li, X;Barlogie, B;Edmondson, R;Yaccoby, S; | Primary myeloma interaction and growth in coculture with healthy donor hematopoietic bone marrow | BMC cancer | 26545722 | itute. NBM coculture system Preparation of the NBM system is illustrated in Fig. 1a. Freshly obtained (via overnight shipping) whole BM cells from healthy adult (>40 years old) donors (n = 20, Allcells, Alameda, CA) were lysed with RBC lysis buffer | Full Paper |
2015 | Liao, W;Jordaan, G;Nham, P;Phan, RT;Pelegrini, M;Sharma, S; | Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens | BMC cancer | 26474785 | In all the CLL specimens, more than 90 % of isolated cells were CD19+ by flow cytometry analysis. Total RNA from isolated B cells (five different normal donors, caucasian males) was purchased from ALLCELLS (Alameda, CA). IGVH mutation (Immunoglobulin | Full Paper |
2015 | Liu, L;Yu, H;Huang, X;Tan, H;Li, S;Luo, Y;Zhang, L;Jiang, S;Jia, H;Xiong, Y;Zhang, R;Huang, Y;Chu, CC;Tian, W; | A novel engineered VEGF blocker with an excellent pharmacokinetic profile and robust anti-tumor activity | BMC cancer | 25881012 | ) in complete HUVEC-adapted medium (Cat#H-004, ALLCELLS) were incubated in 6 cm dishes at 37C, 5% CO 2 for 24 hours, cells were starved for 2 hours and then challenged for 15 Save | Full Paper |
2015 | Priestman, MA;Wang, Q;Jernigan, FE;Chowdhury, R;Schmidt, M;Lawrence, DS; | Multicolor monitoring of the proteasome's catalytic signature | ACS chemical biology | 25347733 | m. All Proteasome inhibitors were purchased from Enzo Life Sciences. HeLa cells were purchased from the tissue culture facility at the University of North Carolina. Normal B-cells were purchased from AllCells. OCI-Ly3 and HBL-1 cells were provided by | Full Paper |
2015 | Yi, Y;Qi, H;Yuan, J;Wang, R;Weng, S;He, J;Dong, C; | Functional characterization of viral tumor necrosis factor receptors encoded by cyprinid herpesvirus 3 (CyHV3) genome | Fish & shellfish immunology | 26052019 | Primary human umbilical vein endothelial cells (HUVECs) were cultured in appropriate media according to the manufacturer's protocol (AllCells, China). The full length of CyHV3 ORF4 Save | Full Paper |
2015 | Doss, JF;Corcoran, DL;Jima, DD;Telen, MJ;Dave, SS;Chi, JT; | A comprehensive joint analysis of the long and short RNA transcriptomes of human erythrocytes | BMC genomics | 26573221 | les as the input list to test for the enrichment of top RBC expressing transcripts against those more specific to D8 samples or the PBMC samples. Cell culture Mobilized Peripheral Blood CD34+ Cells (AllCells) were grown in StemLine II media containi | Full Paper |
2015 | Shi, J;Zhou, J;Zhang, M; | Microcystins induces vascular inflammation in human umbilical vein endothelial cells via activation of NF-B | Mediators of Inflammation | Primary HUVECs were obtained from Allcells (Shanghai, China) and cells in passages 3-5 were used in this study. Cells were grown in an RPMI 1640 medium containing 20% fetal Save | Full Paper | |
2015 | Zhang, Z;Luo, X;Xu, H;Wang, L;Jin, X;Chen, R;Ren, X;Lu, Y;Fu, M;Huang, Y;He, J;Fan, Z; | Bone marrow stromal cell-derived extracellular matrix promotes osteogenesis of adipose-derived stem cells | Cell biology international | 25264269 | BM-MSCs were purchased from ALLCELLS (Emeryville, CA) and expanded in culture medium [α-Minimal Essential Medium (Life Technologies, Grand Island, NY) supplemented with Save | Full Paper |
2015 | Zhang, Q;Wang, LQ;Wong, KY;Li, ZY;Chim, CS; | Infrequent DNA methylation of miR-9-1 and miR-9-3 in multiple myeloma | Journal of clinical pathology | 25855800 | peripheral blood (PB) and three BM normal buffy coat), one CD138-sorted healthy marrow plasma cells (AllCells, California, USA), 10 cell lines (KMS-12-PE, LP-1, MOLP-8, NCI-H929, OPM-2, RPMI-8226, U-266, WL-2, OCI-MY5 and JJN-3), and primary MM marro | Full Paper |
2015 | Wang, DY;Gopinath, S;Lagacé, RE;Norona, W;Hennessy, LK;Short, ML;Mulero, JJ; | Developmental validation of the GlobalFiler(®) Express PCR Amplification Kit: A 6-dye multiplex assay for the direct amplification of reference samples | Forensic science international. Genetics | 26226223 | To demonstrate the sensitivity of the GlobalFiler Express Kit, white blood cells (WBCs) from two individuals were purchased from AllCells, LLC (Emeryville, CA) and quantitated using Save | Full Paper |
2015 | Mahajan, S;Hogan, JK;Shlyakhter, D;Oh, L;Salituro, FG;Farmer, L;Hoock, TC; | VX-509 (decernotinib) is a potent and selective janus kinase 3 inhibitor that attenuates inflammation in animal models of autoimmune disease | The Journal of pharmacology and experimental therapeutics | 25762693 | Sigma-Aldrich (St. Louis, MO); human B cells from AllCells (Emeryville, CA); Roswell Park Memorial Institute medium tissue culture medium, phosphate-buffered saline (PBS), and 10,000 U/ml penicillin-streptomycin from Life Technologies (Grand Island, | Full Paper |
2015 | Shabbir, A;Cox, A;Rodriguez-Menocal, L;Salgado, M;Van Badiavas, E; | Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In Vitro | Stem cells and development | 25867197 | Human MSCs were isolated and expanded from normal donor bone marrow acquired from AllCells LLC (Emeryville, CA, www.allcells.com). Three different donors were used for these experiments. MSCs were isolated by the plastic adherence method as described | Full Paper |
2015 | Limb, JK;Song, D;Jeon, M;Han, SY;Han, G;Jhon, GJ;Bae, YS;Kim, J; | 2-(trimethylammonium)ethyl (R)-3-methoxy-3-oxo-2-stearamidopropyl phosphate promotes megakaryocytic differentiation of myeloid leukaemia cells and primary human CD34⁺ haematopoietic stem cells | Journal of tissue engineering and regenerative medicine | 23166016 | Human bone marrow-derived CD34+ cells were obtained from AllCells (Emeryville, CA, USA). Cells were plated at 8 × 104 cells/well in a 12-well plate and cultured for 16 days in Iscove's modified Dulbecco's medium (IMDM; Invitrogen) with 15% BIT 9500 ( | Full Paper |
2015 | Leelakanok, N;Fischer, CL;Bates, AM;Guthmiller, JM;Johnson, GK;Salem, AK;Brogden, KA;Brogden, NK; | Cytotoxicity of HBD3 for dendritic cells, normal human epidermal keratinocytes, hTERT keratinocytes, and primary oral gingival epithelial keratinocytes in cell culture conditions | Toxicology letters | 26367466 | Human β-defensin 3 (HBD3) is a prominent host defense peptide. In our recent work, we observed that HBD3 modulates pro-inflammatory agonist-induced chemokine and cytokine Save | Full Paper |
2015 | Gupta, MS;Nicoll, SB; | Duration of TGF-β3 Exposure Impacts the Chondrogenic Maturation of Human MSCs in Photocrosslinked Carboxymethylcellulose Hydrogels | Annals of biomedical engineering | 25384834 | Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density centrifugation as previously described.15,28 These primary cells were then cultured using MSC growth medium containing Low Glucose Dulbecco’s Modified Eagle M | Full Paper |
2015 | Marino, MP;Panigaj, M;Ou, W;Manirarora, J;Wei, CH;Reiser, J; | A scalable method to concentrate lentiviral vectors pseudotyped with measles virus glycoproteins | Gene therapy | 25608718 | Human peripheral blood mononuclear cells were obtained from AllCells, LLC (Alameda, CA, USA). T cells were enriched by negative selection using the EasySep Human T Cell Enrichment Cocktail and EasySep D Magnetic Particles (Stem Cell Technologies, Van | Full Paper |
2015 | Shahaduzzaman, MD;Mehta, V;Golden, JE;Rowe, DD;Green, S;Tadinada, R;Foran, EA;Sanberg, PR;Pennypacker, KR;Willing, AE; | Human umbilical cord blood cells induce neuroprotective change in gene expression profile in neurons after ischemia through activation of Akt pathway | Cell transplantation | 25413246 | HUCB Cell Preparation Just prior to the OGD experiment, cryopreserved HUCB mononuclear cells (AllCells, LLC, Alameda, CA, USA) were thawed in a 37ºC water bath and then washed twice in 10 ml PBS (136.9 mM NaCl, 2.7 mM KCl, 10.1 mM Na2HPO4, 1.8 mM KH2 | Full Paper |
2015 | Fukui, T;Mifune, Y;Matsumoto, T;Shoji, T;Kawakami, Y;Kawamoto, A;Ii, M;Akimaru, H;Kuroda, T;Horii, M;Yokoyama, A;Alev, C;Kuroda, R;Kurosaka, M;Asahara, T; | Superior Potential of CD34-Positive Cells Compared to Total Mononuclear Cells for Healing of Nonunion Following Bone Fracture | Cell transplantation | 24800622 | We purchased human GM-PB CD34+ cells obtained from three healthy males (20-year-old African-American; 19-year-old Caucasian; 21-year-old Caucasian) and human GM-PB MNCs obtained from three healthy males (25-year-old African-American; 25-year-old Afri | Full Paper |
2015 | Wada, A;Ito, A;Iitsuka, H;Tsuneyama, K;Miyazono, T;Murakami, J;Shibahara, N;Sakurai, H;Saiki, I;Nakayama, T;Yoshie, O;Koizumi, K;Sugiyama, T; | Role of chemokine CX3CL1 in progression of multiple myeloma via CX3CR1 in bone microenvironments | Oncology reports | 25962684 | GT-3′ and antisense, 5′-CAT GTG GGC CAT GAG GTC CAC CAC-3′. The primers for CX3CR1 were as follows: sense, 5′-TGG CCT TGT CTG ATC TGC TGT TTG-3′ and antisense, 5′-ATG GCT TTG GCT TTC TTG TGG TTC-3′. The RNA from healthy donor plasma cell was purchase | Full Paper |
2015 | Huang, Q;Zhou, C;Chen, X;Dong, B;Chen, S;Zhang, N;Liu, Y;Li, A;Yao, M;Miao, J;Li, Q;Wang, Z; | Prodrug AST-003 Improves the Therapeutic Index of the Multi-Targeted Tyrosine Kinase Inhibitor Sunitinib | PloS one | 26513662 | Dr. Jiang Li at the Sun Yat-Sen University School of Medicine, which were purchased from Type Culture Collection of the Chinese Academy of Sciences in Shanghai, China. HUVEC cells were purchased from Allcells, Inc. The cell lines A549, Caki-1, HT-29, | Full Paper |
2015 | Lanman, RB;Mortimer, SA;Zill, OA;Sebisanovic, D;Lopez, R;Blau, S;Collisson, EA;Divers, SG;Hoon, DS;Kopetz, ES;Lee, J;Nikolinakos, PG;Baca, AM;Kermani, BG;Eltoukhy, H;Talasaz, A; | Analytical and Clinical Validation of a Digital Sequencing Panel for Quantitative, Highly Accurate Evaluation of Cell-Free Circulating Tumor DNA | PloS one | 26474073 | To assess the accuracy of our test, two micrograms of genomic DNA from 20 healthy and young donors (AllCells, Alameda, CA) were sent to an outside reference lab (Ambry Genetics, Save | Full Paper |
2015 | Breese, EH;Buechele, C;Dawson, C;Cleary, ML;Porteus, MH; | Use of Genome Engineering to Create Patient Specific MLL Translocations in Primary Human Hematopoietic Stem and Progenitor Cells | PloS one | 26351841 | When fresh umbilical cord blood was not available, commercially available frozen human CD34+ cells isolated from umbilical cord blood were used (AllCells LLC or StemCell Save | Full Paper |
2015 | Bose, C;Megyesi, JK;Shah, SV;Hiatt, KM;Hall, KA;Karaduta, O;Swaminathan, S; | Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis | PloS one | 26305890 | ked immunosorbent assay (ELISA) by a commercially available kit (GenWay Biotech Inc., San Diego, CA) as instructed by the manufacturer. Cell culture and treatments Commercially available human PBMC (AllCells, Emeryville, CA) were used for in vitro s | Full Paper |
2015 | Ge, HY;Han, ZJ;Tian, P;Sun, WJ;Xue, DX;Bi, Y;Yang, ZH;Liu, P; | VEGFA Expression Is Inhibited by Arsenic Trioxide in HUVECs through the Upregulation of Ets-2 and miRNA-126 | PloS one | 26274316 | , Shanghai, China) were cultured in complete medium (HUVEC-004, supplied by AllCells) in an atmosphere containing 5% CO 2 at 37C. All experiments were performed according to Save | Full Paper |
2015 | Ninomiya, S;Kawano, M;Abe, T;Ishikawa, T;Takahashi, M;Tamura, M;Takahashi, Y;Nashimoto, M; | Potential small guide RNAs for tRNase ZL from human plasma, peripheral blood mononuclear cells, and cultured cell lines | PloS one | 25730316 | myeloma patients (MM1-MM3) were also obtained from AllCells. Written informed consent was obtained from each person by AllCells. In the case that human samples to be analyzed Save | Full Paper |
2015 | Liu, Z;Benard, O;Syeda, MM;Schuster, VL;Chi, Y; | Inhibition of Prostaglandin Transporter (PGT) Promotes Perfusion and Vascularization and Accelerates Wound Healing in Non-Diabetic and Diabetic Rats | PloS one | 26230411 | lonal antibody (LifeSpan BioSciences, Seattle, WA, USA) interacting with rat tissues was used and the dilution was 1: 250. Cell Culture Fresh primary human bone marrow CD34+ cells were obtained from AllCells (Alameda, CA, USA) and cultured according | Full Paper |
2015 | Placencio, VR;Ichimura, A;Miyata, T;DeClerck, YA; | Small Molecule Inhibitors of Plasminogen Activator Inhibitor-1 Elicit Anti-Tumorigenic and Anti-Angiogenic Activity | PloS one | 26207899 | HUVEC (human umbilical vein endothelial cells) were purchased from AllCells and maintained as recommended. HT1080 and HCT116 cells were cultured in DMEM medium Save | Full Paper |
2015 | Fujii, H;Luo, ZJ;Kim, HJ;Newbigging, S;Gassas, A;Keating, A;Egeler, RM; | Humanized Chronic Graft-versus-Host Disease in NOD-SCID il2rγ-/- (NSG) Mice with G-CSF-Mobilized Peripheral Blood Mononuclear Cells following Cyclophosphamide and Total Body Irradiation | PloS one | 26176698 | G-CSF-mobilized human peripheral blood mononuclear cells (G-hPBMCs; 3 donors) and CD34 + cells from G-CSF-mobilized human PB were purchased from ALLCELLS (Emeryvill, CA Save | Full Paper |
2015 | Yan, M;Wen, J;Liang, M;Lu, Y;Kamata, M;Chen, IS; | Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAs | PloS one | 26035832 | edium (Life Technologies, Carlsbad, CA) containing 10% FBS and supplemented with 2mM glutamine, 100 U/ml of penicillin, and 100 μg/ml of streptomycin. Mobilized human CD34+ hematopoietic stem cells (AllCells, Alameda, CA) were cultured in StepSpan SF | Full Paper |
2015 | Patterson, SD;Rossi, JM;Paweletz, KL;Fitzpatrick, VD;Begley, CG;Busse, L;Elliott, S;McCaffery, I; | Functional EpoR pathway utilization is not detected in primary tumor cells isolated from human breast, non-small cell lung, colorectal, and ovarian tumor tissues | PloS one | 25807104 | were adjusted to 106 viable cells/mL prior to growth factor stimulation. For additional cell culture conditions, see S1 Appendix. Erythroid Progenitor-Cell (EPC) Assay Human CD34+ progenitor cells (AllCells Inc., Emeryville, CA) were isolated from b | Full Paper |
2015 | Yu, L;Croze, E;Yamaguchi, KD;Tran, T;Reder, AT;Litvak, V;Volkert, MR; | Induction of a unique isoform of the NCOA7 oxidation resistance gene by interferon β-1b | Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research | 25330068 | Peripheral blood mononuclear cells (PBMCs) derived from healthy individuals were obtained from AllCells. PBMCs (1×106cells/mL) and cultured cells were incubated at 37°C and 5.0% CO2 and grown in Eagle's MEM supplemented with 10% v/v heat-inactivated | Full Paper |
2015 | Liang, X;Potter, J;Kumar, S;Zou, Y;Quintanilla, R;Sridharan, M;Carte, J;Chen, W;Roark, N;Ranganathan, S;Ravinder, N;Chesnut, JD; | Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection | Journal of biotechnology | 26003884 | CD34+ cord blood cells were purchased from AllCells. A549 cells, U-2 OS cells, Neuro-2a (N2A) cells were purchased from ATCC. MEF feeder cells and ROCK inhibitor Y-27632 were Save | Full Paper |
2015 | Kempema, AM;Widen, JC;Hexum, JK;Andrews, TE;Wang, D;Rathe, SK;Meece, FA;Noble, KE;Sachs, Z;Largaespada, DA;Harki, DA; | Synthesis and antileukemic activities of C1-C10-modified parthenolide analogues | Bioorganic & medicinal chemistry | 26088334 | Frozen human mononuclear bone marrow cells were purchased from AllCells (Cat. #ABM011F). These bone marrow cells were from two donors (#5630 [Lot #BM4565] and #4887 [Lot # Save | Full Paper |
2015 | Saraf, S;Araki, H;Petro, B;Park, Y;Taioli, S;Yoshinaga, KG;Koca, E;Rondelli, D;Mahmud, N; | Ex vivo expansion of human mobilized peripheral blood stem cells using epigenetic modifiers | Transfusion | 25363624 | Growth factor-mobilized human MPB or BM cells were obtained from healthy donors either from a commercially available source (AllCells, LLC, Emeryville, CA) or from aliquots of Save | Full Paper |
2015 | Geiger, A;Walker, A;Nissen, E; | Human fibrocyte-derived exosomes accelerate wound healing in genetically diabetic mice | Biochemical and biophysical research communications | 26454169 | Human donor fibrocytes were obtained from leukapheresis packs (AllCells), as previously reported for experiments requiring a high yield of these cells [5], [16], [28]. Viable CD45+[CD3/CD19/CD20/CRTH2]−CD16−CD115−CD11b+CD34+ fibrocytes were enumerate | Full Paper |
2015 | Takahashi, T;Katano, I;Ito, R;Ito, M; | Visualization of the human CD4⁺ T-cell response in humanized HLA-DR4-expressing NOD/Shi-scid/γc(null) (NOG) mice by retrogenic expression of the human TCR gene | Biochemical and biophysical research communications | 25462565 | 2.2. CD34+ hematopoietic stem cells Umbilical CD34+ cells were obtained from AllCells (Alameda, CA). 2.3. DNA constructs BLG-specific CD4+ T-cell clones (HA5.7 and YA4) were kindly provided by Dr. Kondo [14]. The TCRα and β genes were isolated and t | Full Paper |
2015 | Antebi, B;Zhang, Z;Wang, Y;Lu, Z;Chen, XD;Ling, J; | Stromal-cell-derived extracellular matrix promotes the proliferation and retains the osteogenic differentiation capacity of mesenchymal stem cells on three-dimensional scaffolds | Tissue engineering. Part C, Methods | 24965227 | Primary bone-marrow-derived mononuclear cells (MNCs) from human donors 20-30 years of age were purchased from AllCells, LLC (Emeryville, CA). MNCs were cultured on standard Save | Full Paper |
2015 | Akiyama, H;Kobayashi, A;Ichimura, M;Tone, H;Nakatani, M;Inoue, M;Tojo, A;Kagami, H; | Comparison of manual and automated cultures of bone marrow stromal cells for bone tissue engineering | Journal of bioscience and bioengineering | 25907575 | Fresh human bone marrows, obtained from three healthy donors (25- and 28-year-old males and a 38-year-old female), were purchased from AllCells (Emeryville, CA, USA). Each bone marrow was divided into two 20-mL groups, and the manual and automated pr | Full Paper |
2015 | Natu, VP;Dubey, N;Loke, GC;Tan, TS;Ng, WH;Yong, CW;Cao, T;Rosa, V; | Bioactivity, physical and chemical properties of MTA mixed with propylene glycol | Journal of applied oral science : revista FOB | 26398513 | paration of MTA conditioned media The use of human dental pulp stem cells in this study was approved by the Institutional Review Board/NUS (Approval Number: NUS 2094). DPSCs between passages 3 and 6 (Allcells, Alameda, California, USA) were used to e | Full Paper |
2015 | Musa, M;Mohd Ali, K;Kannan, TP;Azlina, A;Omar, NS;Chatterji, A;Mokhtar, KI; | Effects of Perivitelline Fluid Obtained from Horseshoe Crab on The Proliferation and Genotoxicity of Dental Pulp Stem Cells | Cell journal | 26199904 | xicity was evaluated based on the chromosome aberration (CA) and Ames tests. Materials and Methods Study design This was an in vitro experimental study carried out on DPSCs. Cell culture DPSCs from AllCells (USA, cat no. DP004F) were cultured in me | Full Paper |
2015 | Elorza, MT; | Human cytomegalovirus genome replication and maintenance during latent infection | Thesis | Human CD14 (+) cells purchased (Lonza and ReachBio) or isolated from cord blood were maintained in Iscove DMEM (Hyclone) supplemented with 20% heatinactivated FBS (Atlanta Biologicals), 50 ng/mL M-CSF, 50 ng/mL stem cell factor (SCF), 50 ng/mL G-CSF, | Full Paper | |
2015 | Kamocki, K;Nör, JE;Bottino, MC; | Effects of ciprofloxacin-containing antimicrobial scaffolds on dental pulp stem cell viability-In vitro studies | Archives of oral biology | 26042622 | % penicillin-streptomycin (Sigma-Aldrich) placed in a humidified incubator at 37°C, with 5% CO2, was used to culture human dental pulp stem cells (hDPSC, AllCells LLC, Alameda, CA, USA) obtained from permanent third molars. 17,18 The medium was chang | Full Paper |
2015 | Guo, J;Wang, Q;Wai, D;Zhang, QZ;Shi, SH;Le, AD;Shi, ST;Yen, SL; | Visible red and infrared light alters gene expression in human marrow stromal fibroblast cells | Orthodontics & craniofacial research | 25865533 | Commercially available human bone marrow aspirates from two healthy adult donors were purchased from AllCells (Emeryville, CA). Human bone marrow stem cells (HBMSCs) were isolated from the aspirates following published protocols10, 11 and then cultur | Full Paper |
2015 | Thys, RG;Lehman, CE;Pierce, LC;Wang, YH; | Environmental and chemotherapeutic agents induce breakage at genes involved in leukemia-causing gene rearrangements in human hematopoietic stem/progenitor cells | Mutation research | 26163765 | ot been previously connected to leukemogenesis. 2. Materials and methods 2.1. Cells and culture conditions Experiments were performed using primary human CD34+ bone marrow stem and progenitor cells (ALLCELLS), grown in Hematopoietic Growth Medium (L | Full Paper |
2015 | Low, A;Mohd Yusof, H;Reza, F;Abdullah Nurul, A;Sritharan, S;Haania Zain Ali, N;Subhi Azeez, H;Husein, A; | Gypsum-based biomaterials: Evaluation of physical and mechanical properties, cellular effects and its potential as a pulp liner | Dental materials journal | 26235719 | SHED (AllCells, Chicago, IL, USA) were cultivated under sterile conditions in culture flasks containing growth media in a controlled condition at 37°C under a 5% CO2 humidified atmosphere. The culture media consisted of 10% heat-inactivated fetal bov | Full Paper |
2015 | Zhan, H;Ciano, K;Dong, K;Zucker, S; | Targeting glutamine metabolism in myeloproliferative neoplasms | Blood cells, molecules & diseases | 26227854 | Peripheral blood CD34 + cells from healthy controls were purchased from AllCells . Peripheral blood CD34 + cells from healthy controls were purchased from AllCells . Save | Full Paper |
2015 | Ciaffoni, F;Cassella, E;Varricchio, L;Massa, M;Barosi, G;Migliaccio, AR; | Activation of non-canonical TGF-β1 signaling indicates an autoimmune mechanism for bone marrow fibrosis in primary myelofibrosis | Blood cells, molecules & diseases | 25703685 | Signalling profiling was performed on mononuclear cells obtained from BM (n=3) and spleen (n=6) of patients, normal BM (n=3) (ABM008F-BM3366, ABM008F-BM3527 and ABM008F-BM3600, ALLCELLS, Emeryville, CA) and spleen (n=3) from males (30-years old) who | Full Paper |
2015 | Nazarian, AA;Archibeque, IL;Nguyen, YH;Wang, P;Sinclair, AM;Powers, DA; | Characterization of bispecific T-cell Engager (BiTE) antibodies with a high-capacity T-cell dependent cellular cytotoxicity (TDCC) assay | Journal of biomolecular screening | 25477202 | Normal peripheral blood (NPB) was collected via leukapheresis from normal healthy human donors who were nonsmokers and were not taking any allergy medication (AllCells, Save | Full Paper |
2015 | Suknuntha, K;Ishii, Y;Tao, L;Hu, K;McIntosh, BE;Yang, D;Swanson, S;Stewart, R;Wang, JYJ;Thomson, J;Slukvin, I; | Discovery of survival factor for primitive chronic myeloid leukemia cells using induced pluripotent stem cells | Stem cell research | 26561938 | Bone marrow mononuclear cells from CML patients in the chronic phase were purchased commercially (AllCells or Applied StemCells), or obtained from the patients at the University of Save;w specimens from CML patients in the chronic phase, and contro | Full Paper |
2015 | Kanjevac, T;Milovanovic, M;Milosevic-Djordjevic, O;Tesic, Z;Ivanovic, M;Lukic, A; | Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations | Archives of Biological Sciences | Human SHED purchased from AllCells, LLC (Emeryville, California, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS, 100 I.U./mL penicillin G and 100 μg/mL streptomycin (Sigma-Aldrich | Full Paper | |
2015 | Gonçalves Silva, I;Gibbs, BF;Bardelli, M;Varani, L;Sumbayev, VV; | Differential expression and biochemical activity of the immune receptor Tim-3 in healthy and malignant human myeloid cells | Oncotarget | 26413815 | e highest grade of purity and available commercially unless otherwise stated. Primary human AML cells Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accorda | Full Paper |
2015 | Gibbs, BF;Gonçalves Silva, I;Prokhorov, A;Abooali, M;Yasinska, IM;Casely-Hayford, MA;Berger, SM;Fasler-Kan, E;Sumbayev, VV; | Caffeine affects the biological responses of human hematopoietic cells of myeloid lineage via downregulation of the mTOR pathway and xanthine oxidase activity | Oncotarget | 26384306 | For this purpose we used primary acute myeloid leukaemia cells AML-PB001F purchased from AllCells (Alameda, CA, USA). Cells were exposed to 0.1 μg/ml SCF (these cells express Save | Full Paper |
2015 | Wang, Y;Li, JX;Wang, YQ;Miao, ZH; | Tanshinone I inhibits tumor angiogenesis by reducing Stat3 phosphorylation at Tyr705 and hypoxia-induced HIF-1α accumulation in both endothelial and tumor cells | Oncotarget | 26202747 | ere obtained from the French National Institute of Health and Medical Research (INSERM, FR), and cultured with complete MCDB131 medium (Invitrogen, Carlsbad, CA, USA). HUVEC cells were purchased from AllCells (Shanghai, China). Human breast cancer MC | Full Paper |
2015 | Liu, K;Liu, X;Peng, Z;Sun, H;Zhang, M;Zhang, J;Liu, S;Hao, L;Lu, G;Zheng, K;Gong, X;Wu, D;Wang, F;Shen, L; | Retargeted human avidin-CAR T cells for adoptive immunotherapy of EGFRvIII expressing gliomas and their evaluation via optical imaging | Oncotarget | 26124178 | To isolate peripheral blood mononuclear Cells (PBMC), the red blood cells and platelets were removed with PBMC isolation kits according to the manufacturer's instruction (AllCells, Save | Full Paper |
2015 | Ao, JY;Chai, ZT;Zhang, YY;Zhu, XD;Kong, LQ;Zhang, N;Ye, BG;Cai, H;Gao, DM;Sun, HC; | Robo1 promotes angiogenesis in hepatocellular carcinoma through the Rho family of guanosine triphosphatases' signaling pathway | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | 26022159 | Robo1 is a member of the Robo immunoglobulin superfamily of proteins, and it plays an important role in angiogenesis and cancer. In this study, we investigate the role of roundabout 1 Save | Full Paper |
2015 | Patel, P;Mahmud, D;Park, Y;Yoshinaga, K;Mahmud, N;Rondelli, D; | Clinical grade isolation of regulatory T cells from G-CSF mobilized peripheral blood improves with initial depletion of monocytes | American journal of blood research | 27069755 | Tregs were purified from G-PBSC (AllCells) obtained from healthy donors. In some cases CD34 + cells were initially isolated on a ClinMACS device (Miltenyi) by positive selection using Save | Full Paper |
2015 | Woodell-May, JE;Tan, ML;King, WJ;Swift, MJ;Welch, ZR;Murphy, MP;McKale, JM; | Characterization of the Cellular Output of a Point-of-Care Device and the Implications for Addressing Critical Limb Ischemia | BioResearch open access | 26634187 | s collected from the iliac crest from healthy financially compensated subjects who qualified for blood donation as per AABB standards. Aspiration was performed by Poietics-Cambrex (Gaithersburg, MD), AllCells (Alameda, CA), and HemaCare (Van Nuys, CA | Full Paper |
2015 | Yao, J;Song, A;Ruan, Z;Zhou, L;Liu, P;Zhu, H;Gong, H;Dong, S;Xu, Y;Jiang, E;Pang, A;Feng, S;Han, M; | [Vascular endothelial injury induced by anti-endothelial cell antibody in allogeneic hematopoietic stem cell transplantation] | Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi | 26134010 | FBS)及BCA定量试剂盒购自美国Thermo Scientific公司;辣 过氧化酶 记的羊抗人IgG购自 美国KPL公司;四甲基联苯胺(TMB)购自美国Tiangen公司;HUVEC及EC完全培养基购自美国Allcells Save | Full Paper |
2015 | Du, C;Weng, X;Hu, W;Lv, Z;Xiao, H;Ding, C;Gyabaah, OA;Xie, H;Zhou, L;Wu, J;Zheng, S; | Hypoxia-inducible MiR-182 promotes angiogenesis by targeting RASA1 in hepatocellular carcinoma | Journal of experimental & clinical cancer research : CR | 26126858 | The HUVECs were obtained from Allcells (Shanghai, China allcells.biomart.cn) and maintained with Serum Free Medium for Endothelial Cells (SFM, Invitrogen) supplemented with 20 Save | Full Paper |
2015 | Kawase, T;Tanaka, T;Okuda, K;Tsuchimochi, M;Oda, M;Hara, T; | Quantitative single-cell motility analysis of platelet-rich plasma-treated endothelial cells in vitro | Cytoskeleton (Hoboken, N.J.) | 25845465 | HUVECs were obtained from AllCells, LLC (Emeryville, CA) and maintained in endothelial cell medium supplemented with growth factors (HuMedia-EB2; KURABO, Osaka, Japan) [Kobayashi et al., 2012]. For the wound healing assay, HUVECs were seeded into dou | Full Paper |
2015 | Fung, C;Mohamad, H;Hashim, S;Htun, A;Ahmad, A; | Proliferative Effect of Malaysian Propolis on Stem Cells from Human Exfoliated Deciduous Teeth: An In vitro Study | British Journal of Pharmaceutical Research | The frozen commercial SHED purchased from AllCells (USA) was retrieved and cultured in alpha minimal essential media (α-MEM) (Gibco, Invitrogen, USA) supplemented with 15% Save | Full Paper | |
2015 | Miura, Y;Ichinohe, T;Maekawa, T; | Human Mesenchymal Stromal/Stem Cell-Mediated Bone Marrow Organization | Japanese Journal of Transfusion and Cell Therapy | In this model, MSCs isolated from bone marrow samples (purchased from AllCells, Emeryville, CA, USA) through the conventional adhesion selection method (BM-MSCs, Fig. A) were Save | Full Paper | |
2015 | Kanazawa, T;Shiba, N;Aizawa, A;Okuno, H;Tamura, K;Tsukada, S;Kumamoto, T;Yamada, S;Kobayashi, Y;Arakawa, H; | Nelarabine Resistance of Childhood T-Cell Lymphoblastic Leukemia/Lymphoma Cells | The Kitakanto Medical Journal | In vitro NEL resistance was seen in half of the testedchildhoodT-ALLcells. ENT-1maypartlyact as a transporter of NEL, but not play a key role in AraC induced NEL resistance. This cell Save | Full Paper | |
2015 | Wu, M;Davis, R;Hatch, A; | Portable microfluidic raman system for rapid, label-free early disease signature detection | Sandia National Laboratories (SNL) | Order blood from Allcells(Alameda, CA), get blood within 4 hours. 2. Morning before blood arrives: 3. Make 50 ml 1x BD lysis buffer (Cat no. 349202), store at 4C. 4. Make 100X stock solutions for stimulation: 1:10 dilution of LPS stock to make 10ug/m | Full Paper | |
2015 | Saka, E; | Image registration and visualization of in situ gene expression images. | University of Louisville | Allcells are built out of similar materials (including an organism's DNA) and function insimilar ways. Prokaryotic cells, such as bacteria and some single celled organisms, havesimple Save | Full Paper | |
2015 | Taha, S;Ling, J;Azmi, N;Kannan, T;Azlina, A;Mokhtar, K; | Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth medium | Brazilian Journal of Oral Sciences | Stem cells from human exfoliated deciduous teeth (SHED) (ALLCells, Alameda, CA, USA) were employed in the current study. SHED was cultured and maintained in T25 cm 2 culture Save | Full Paper | |
2015 | Neises, A;Su, R;Zhang, X; | Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCs | SpringerBriefs in Stem Cells | of water and add 0.9 ml of 5 % EDTA. Adjust the pH to 7.2-7.4. Add water to bring the volume up to 500 ml and filter sterilize through a 0.2-µm filter. Store at 4 °C, but bring to room temperature before use. 4. Alternatively, cord blood CD34+ cells | Full Paper | |
2015 | Doss, J; | The Erythrocyte Transcriptome: Global Characterization and Therapeutic Implication | Thesis | Mobilized Peripheral Blood CD34+ Cells (AllCells) were grown in StemLine II media containing Glutamine (Sigma) with 20% BIT (StemCell Technologies) and 1% Penicillin/Streptomycin (Gibco). On Days 0-7, cells were supplemented with growth factors IL-3 | Full Paper | |
2015 | Ferng, AS; | Cardiac Organogenesis: 3D Bioscaffolds, Bioenergetics and Regeneration | Thesis | Human amniotic stem cells were purchased (AllCells, Alameda, CA) and cultured in Celprogen media (Celprogen, Torrance, CA) under standard cell culture conditions before reseeding on the membrane patches. AmSCs were seeded at 100,000 cells/cm2 per mem | Full Paper | |
2015 | Quarmyne, M; | Protein Tyrosine Phosphatase Receptor Type S (PTPRS) Regulates Hematopoietic Stem Cell Self-Renewal | Thesis | Center according to an approved protocol by the Institutional Review Board of Duke University or the Institutional Biosafety Committee of University of California - Los Angeles. Frozen CB mononuclear cells (MNCs) were obtained from AllCells, LLC., Al | Full Paper | |
2015 | Sanacora, S; | Regulation of IL-8 expression by proteasome in human monocytes and macrophages | Thesis | U937 human promyeloid cells were obtained from American Type Culture Collection (ATCC; Rockville, MD, USA). Peripheral blood mononuclear cells (PBMC) from healthy human volunteers were purchased from AllCells (PB003F; Alameda, CA, USA). The cells wer | Full Paper | |
2015 | Uppal, H;Doudement, E;Mahapatra, K; | Potential Mechanisms for Thrombocytopenia Development with Trastuzumab Emtansine (T-DM1) The Effect of T-DM1 on Platelets and Megakaryocytes | Clin Cancer Res | HSCs (CD133+/CD34+) were purchased from ALLCELLS. Briefly, these HSCs were enriched from bone marrow isolates of healthy donors using positive selection for CD133 and CD34. CD133+/CD34+ cells were first expanded in cytokine-enriched hematopoietic exp | Full Paper | |
2015 | Gupta, MS; | Development of a Carboxymethylcellulose Hydrogel System for Stem Cell-Based Nucleus Pulposus Tissue Engineering | Thesis | Human MSCs were isolated from the bone marrow of a 37 year-old male (AllCells, Emeryville, CA) using enriched density gradient centrifugation. Briefly, a Human MSC Enrichment Cocktail (StemCell Technologies, Vancouver, BC, Canada) (Lee et al. 2005) w | Full Paper | |
2015 | Leon, B; | Discovery, Development, and Applications of Natural Products: Abyssomicin 2, Quinoline Amino Alcohols, and Fluorinated Bile Acids | Thesis | In vitro model of HIV latency. Generation of latent infected cells was performed as previously described.9 Briefly, naive CD4+ T cells were purified by negative selection using PBMCs from healthy HIV negative donors obtained by leukapheresis (AllCell | Full Paper | |
2015 | Kizilyer, A; | The Role of Neurotrophin Signaling in Megakaryocyte Differentiation and Platelet Production | Thesis | CD34+ cells isolated from human bone marrow (BM) or umbilical cord blood were purchased from Allcells (Emeryville, CA, USA), and differentiated according to the previously published protocol [185]. For inducing megakaryocyte progenitor cell different | Full Paper | |
2015 | UCOK, C;KARAAHMETOĞLU, O;UCOK, C; | An analysis of the decision to extract third molar teeth | Thesis | Materials and method: SHED (AllCells, Emeryville, CA; 1 9 105 cells in 2 ml culture medium) were seeded into six-well plates and cultured for 12 h. After the change of fresh medium, LPS (E. coli; 1 lg/ml) or saline (control) was added. Following 3, 1 | Full Paper | |
2015 | OZMERİC KURTULUŞ, N;Selin, A;CANKAL, D; | Treatment of a Patient with Unilateral Cleft Lip and Palate with Free Gingival Greft | Thesis | Materials and method: SHED (AllCells, Emeryville, CA; 1 9 105 cells in 2 ml culture medium) were seeded into six-well plates and cultured for 12 h. After the change of fresh medium, LPS (E. coli; 1 lg/ml) or saline (control) was added. Following 3, 1 | Full Paper | |
2015 | Wang, X;Wei, W;Krzeszinski, JY;Wang, Y;Wan, Y; | A Liver-Bone Endocrine Relay by IGFBP1 Promotes Osteoclastogenesis and Mediates FGF21-Induced Bone Resorption | Cell metabolism | 26456333 | cells were pelleted by centrifugation at 10K rpm for 10min; supernatant was collected as liver-cell-derived conditioned medium (LCM) and used to treat osteoclast differentiation culture. Human PBMN cells (ReachBio) were differentiated into osteoclast | Full Paper |
2015 | Xiang, J;Wu, DC;Chen, Y;Paulson, RF; | In vitro culture of stress erythroid progenitors identifies distinct progenitor populations and analogous human progenitors | Blood | 25608563 | Human bone marrow mononuclear cells were purchased from Reachbio (#033-200) and cultured in the stress erythropoiesis medium described above except that human growth Save | Full Paper |
2015 | Jaramillo, MC;Briehl, MM;Batinic-Haberle, I;Tome, ME; | Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells | Free radical biology & medicine | 25725417 | The manganese porphyrin, manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin (MnTE-2-PyP 5+), acts as a pro-oxidant in the presence of intracellular H 2 O 2. Mitochondria Save;Normal human peripheral mononuclear blood cells (PMBC) were p | Full Paper |
2015 | Grandi, FC;Rosser, JM;Newkirk, SJ;Yin, J;Jiang, X;Xing, Z;Whitmore, L;Bashir, S;Ivics, Z;Izsvák, Z;Ye, P;Yu, YE;An, W; | Retrotransposition creates sloping shores: a graded influence of hypomethylated CpG islands on flanking CpG sites | Genome research | 25995269 | hnology, Paul Ehrlich Institute, 63225 Langen, Germany 6 These authors contributed equally to this work. 7 Present addresses: Cancer Biology Program, Stanford University, Stanford, CA 94305, USA; 8 ReachBio LLC, Seattle, WA 98107, USA; 9 Departmen | Full Paper |
2015 | Purushothaman, P;Thakker, S;Verma, SC; | Transcriptome analysis of Kaposi's sarcoma-associated herpesvirus during de novo primary infection of human B and endothelial cells | Journal of virology | 25552714 | Human PBMCs (ReachBio, WA) were cultured in RPMI 1640 medium (HyClone) supplemented with 10% FBS, 2 mM l-glutamine, and penicillin-streptomycin (5 U/ml and 5 μg/ml, respectively). Human CD14+ cells were isolated from cord blood received from the Colo | Full Paper |
2015 | Panchanathan, R;Liu, H;Leung, YK;Ho, SM;Choubey, D; | Bisphenol A (BPA) stimulates the interferon signaling and activates the inflammasome activity in myeloid cells | Molecular and cellular endocrinology | 26277401 | Cells lysates were prepared and analyzed for the levels of activated caspase-1 and the mature IL-1β as described (Veeranki et al., 2011). Human purified (CD14+) monocytes from a healthy male donor were purchased from ReachBio (Seattle, WA) and cultur | Full Paper |
2015 | D'Alessandro, JS;Duffner, J;Pradines, J;Capila, I;Garofalo, K;Kaundinya, G;Greenberg, BM;Kantor, D;Ganguly, TC; | Equivalent Gene Expression Profiles between Glatopa™ and Copaxone® | PloS one | 26473741 | tiation of the experiment to minimize bias. All samples were tested on the same day to minimize the introduction of bias resulting from sample processing. First, APC 0.5 mL (naive Balb/C splenocytes, ReachBio LLC, Seattle, WA, USA) cell suspension (1 | Full Paper |
2015 | Tarrant, JM;Dhawan, P;Singh, J;Zabka, TS;Clarke, E;DosSantos, G;Dragovich, PS;Sampath, D;Lin, T;McCray, B;La, N;Nguyen, T;Kauss, A;Dambach, D;Misner, DL;Diaz, D;Uppal, H; | Preclinical models of nicotinamide phosphoribosyltransferase inhibitor-mediated hematotoxicity and mitigation by co-treatment with nicotinic acid | Toxicology mechanisms and methods | 25894564 | Human bone marrow (n = 3) (ReachBio, Seattle, WA) or cynomolgus monkey bone marrow During the conduct of this work, authors were employees of either Genentech or ReachBio. Save | Full Paper |
2015 | Cook, G; | Studies of the efficacy of Ribonucleotide Reductase inhibition in preclinical models of Acute Myeloid Leukemia in combination with standard and novel therapeutics | Thesis | Cells were washed with PBS and resuspended in IMDM supplemented with 20% FBS, and placed in ColonyGel High Cytokine Formulation media (ReachBio). Experiments were performed in triplicate. Colonies were counted on or after day 7. Colonies of 8 or more | Full Paper | |
2015 | Thakker, ST; | Dynamics of KSHV gene expression during de novo infection and the role of LANA in immune modulation | Thesis | Erle S. Robertson, were cultured in endothelial basal medium-2 (Lonza) supplemented with endothelial growth factor supplements (Lonza). Human PBMCs (ReachBio, WA) were cultured in RPMI 1640 medium (Hyclone) supplemented with 10% FBS, 2 mM L-glutamine | Full Paper | |
2014 | Li, S;Tighe, SW;Nicolet, CM;Grove, D;Levy, S;Farmerie, W;Viale, A;Wright, C;Schweitzer, PA;Gao, Y;Kim, D;Boland, J;Hicks, B;Kim, R;Chhangawala, S;Jafari, N;Raghavachari, N;Gandara, J;Garcia-Reyero, N;Hendrickson, C;Roberson, D;Rosenfeld, J;Smith, T;Underwood, JG;Wang, M;Zumbo, P;Baldwin, DA;Grills, GS;Mason, CE; | Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study | Nat. Biotechnol. | 25150835 | Department of Physiology and Biophysics, Weill Cornell Medical College, New York, New York, USA.++Vermont Cancer Center, University of Vermont, Burlington, Vermont, USA.++Keck School of Medicine, University of Southern California, Los Angeles, Califo | Full Paper |
2014 | Graves, JD;Kordich, JJ;Huang, TH;Piasecki, J;Bush, TL;Sullivan, T;Foltz, IN;Chang, W;Douangpanya, H;Dang, T;O'Neill, JW;Mallari, R;Zhao, X;Branstetter, DG;Rossi, JM;Long, AM;Huang, X;Holland, PM; | Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activity | Cancer Cell | 25043603 | Non-small-cell lung tumor samples were collected under Institutional Review Board approval for each institution, with appropriate informed consent, from surgical resections or after biopsy. Freshly resected human tissue samples were obtained from the | Full Paper |
2014 | Brissova, M;Aamodt, K;Brahmachary, P;Prasad, N;Hong, JY;Dai, C;Mellati, M;Shostak, A;Poffenberger, G;Aramandla, R;Levy, SE;Powers, AC; | Islet microenvironment, modulated by vascular endothelial growth factor-A signaling, promotes β cell regeneration | Cell Metab. | 24561261 | Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA. Electronic address: marcela.brissova@vanderbilt.edu.++Department of Molecular Physiology and Biophysics, Vand | Full Paper |
2014 | Locke, D;Lynch, F;Bernstein, S;Siami-Namini, K;Yarranton, G; | An IHC Screen for EphA3 Positive Myelofibrosis (MF) and Other Myeloproliferative Neoplasms (MPNs) | Blood | Neoplasms (MPNs). Darren Locke, PhD Darren Locke, PhD. 1. QualTek Molecular Laboratories, Newtown,. 1. Search for other works by this author on: This Site. PubMed MPNs. Disclosures. Locke:QualTek Molecular Labs: Employment. Lynch | Full Paper | |
2014 | Fecteau, JF;Corral, LG;Ghia, EM;Gaidarova, S;Futalan, D;Bharati, IS;Cathers, B;Schwaederlé, M;Cui, B;Lopez-Girona, A;Messmer, D;Kipps, TJ; | Lenalidomide inhibits the proliferation of CLL cells via a cereblon/p21(WAF1/Cip1)-dependent mechanism independent of functional p53 | Blood | 24990888 | Blood samples were collected from CLL patients at the University of California San Diego Moores Cancer Center who satisfied diagnostic and immunophenotypic criteria for common B-cell CLL, and who provided written, informed consent, in compliance with | Full Paper |
2014 | Sugihara, K;Kobayashi, Y;Suzuki, A;Tamura, N;Motamedchaboki, K;Huang, CT;Akama, TO;Pecotte, J;Frost, P;Bauer, C;Jimenez, JB;Nakayama, J;Aoki, D;Fukuda, MN; | Development of pro-apoptotic peptides as potential therapy for peritoneal endometriosis | Nat Commun | 25047118 | ... wa (positive) and A431 (negative) cells. A hybridoma clone was subcloned by a limited dilution, establishing the 3B2 clone. Paraffin tissue sections endometriosis patients (_n_=35) were obtained from Folio Biosciences (Columbus, OH). A normal ute | Full Paper |
2014 | Ramesh, R;Kozhaya, L;McKevitt, K;Djuretic, IM;Carlson, TJ;Quintero, MA;McCauley, JL;Abreu, MT;Unutmaz, D;Sundrud, MS; | Pro-inflammatory human Th17 cells selectively express P-glycoprotein and are refractory to glucocorticoids | J. Exp. Med. | 24395888 | ... sue samples. All experiments using human blood and tissue samples were conducted in accordance with IRB protocols approved by committees at Research Blood Components, New York Blood Center, OneBlood, Conversant Bio., and the University of Miami. | Full Paper |
2014 | Van Bortle, K;Nichols, MH;Li, L;Ong, CT;Takenaka, N;Qin, ZS;Corces, VG; | Insulator function and topological domain border strength scale with architectural protein occupancy | Genome Biol. | 24981874 | We are especially thankful to Drs Dale Dorsett, Hugo Bellen, Giovanni Bosco, and Jørgen Johansen for graciously sharing antibodies against Rad21, Barren, CAP-H2, and Chromator. We thank the Drosophila Genomic Resource Center (supported by NIH grant O | Full Paper |
2014 | Steffen, MM;Dearth, SP;Dill, BD;Li, Z;Larsen, KM;Campagna, SR;Wilhelm, SW; | Nutrients drive transcriptional changes that maintain metabolic homeostasis but alter genome architecture in Microcystis | ISME J | 24858783 | ; Scientific, Waltham, MA, USA). Subsequent sample processing, including rRNA subtraction, library prep and sequencing, were performed by the HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA). RNA quality was | Full Paper |
2014 | Picard, M;Zhang, J;Hancock, S;Derbeneva, O;Golhar, R;Golik, P;O'Hearn, S;Levy, S;Potluri, P;Lvova, M;Davila, A;Lin, CS;Perin, JC;Rappaport, EF;Hakonarson, H;Trounce, IA;Procaccio, V;Wallace, DC; | Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional reprogramming | Proc. Natl. Acad. Sci. U.S.A. | 25192935 | Center for Mitochondrial and Epigenomic Medicine, Children's Hospital of Philadelphia and the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104;++School of Biological Sciences, The University of Hong | Full Paper |
2014 | Smith, DC;Eisenberg, PD;Manikhas, G;Chugh, R;Gubens, MA;Stagg, RJ;Kapoun, AM;Xu, L;Dupont, J;Sikic, B; | A phase I dose escalation and expansion study of the anticancer stem cell agent demcizumab (anti-DLL4) in patients with previously treated solid tumors | Clin. Cancer Res. | 25324140 | In addition, control plasma and whole blood samples were sourced and collected (Conversant Bio) using the same methods described above from 8 cancer patients who were not treated with demcizumab. Affymetrix human gene chip U133 Plus 2.0 arrays were u | Full Paper |
2014 | Gee, HY;Ashraf, S;Wan, X;Vega-Warner, V;Esteve-Rudd, J;Lovric, S;Fang, H;Hurd, TW;Sadowski, CE;Allen, SJ;Otto, EA;Korkmaz, E;Washburn, J;Levy, S;Williams, DS;Bakkaloglu, SA;Zolotnitskaya, A;Ozaltin, F;Zhou, W;Hildebrandt, F; | Mutations in EMP2 cause childhood-onset nephrotic syndrome | Am. J. Hum. Genet. | 24814193 | Division of Nephrology, Department of Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA.++Division of Nephrology, Department of Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, US | Full Paper |
2014 | Gee, HY;Otto, EA;Hurd, TW;Ashraf, S;Chaki, M;Cluckey, A;Vega-Warner, V;Saisawat, P;Diaz, KA;Fang, H;Kohl, S;Allen, SJ;Airik, R;Zhou, W;Ramaswami, G;Janssen, S;Fu, C;Innis, JL;Weber, S;Vester, U;Davis, EE;Katsanis, N;Fathy, HM;Jeck, N;Klaus, G;Nayir, A;Rahim, KA;Al Attrach, I;Al Hassoun, I;Ozturk, S;Drozdz, D;Helmchen, U;O'Toole, JF;Attanasio, M;Lewis, RA;Nürnberg, G;Nürnberg, P;Washburn, J;MacDonald, J;Innis, JW;Levy, S;Hildebrandt, F; | Whole-exome resequencing distinguishes cystic kidney diseases from phenocopies in renal ciliopathies | Kidney Int. | 24257694 | Division of Nephrology, Department of Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.++Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, USA.++MRC Human Genetics | Full Paper |
2014 | Rosenfeldt, MT;Bell, LA;Long, JS;O'Prey, J;Nixon, C;Roberts, F;Dufès, C;Ryan, KM; | E2F1 drives chemotherapeutic drug resistance via ABCG2 | Oncogene | 24276245 | Biotechnology (St Louis, MO, USA). The ChIP Assay Kit was from Millipore (Watford, UK) and the human lung cancer tissue microarray was from Folio Biosciences (Columbus, OH, USA). Plasmids. pTRE-E2F1 constructs were generated | Full Paper |
2014 | Wildsmith, KR;Schauer, SP;Smith, AM;Arnott, D;Zhu, Y;Haznedar, J;Kaur, S;Mathews, WR;Honigberg, LA; | Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomics | Mol Neurodegener | 24902845 | ... ap in clinical biomarker development. Methods Source of CSF CSF was purchased from Bioreclamation, LLC (Hicksville, NY) (pooled cynomolgus monkey and pooled human CSF used in discovery experiments), Folio Biosciences (Columbus, OH) (individual l | Full Paper |
2014 | Farber, CR;Reich, A;Barnes, AM;Becerra, P;Rauch, F;Cabral, WA;Bae, A;Quinlan, A;Glorieux, FH;Clemens, TL;Marini, JC; | A novel IFITM5 mutation in severe atypical osteogenesis imperfecta type VI impairs osteoblast production of pigment epithelium-derived factor | J. Bone Miner. Res. | 24519609 | Exome sequencing was performed by the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology (Huntsville, AL, USA). Briefly, gDNA (1 to 2 μg) was fragmented and subjected to exome enrichment using the Nimblegen SeqCap EZ Human Exome Libr | Full Paper |
2014 | Shipitsin, M;Small, C;Choudhury, S;Giladi, E;Friedlander, S;Nardone, J;Hussain, S;Hurley, AD;Ernst, C;Huang, YE;Chang, H;Nifong, TP;Rimm, DL;Dunyak, J;Loda, M;Berman, DM;Blume-Jensen, P; | Identification of proteomic biomarkers predicting prostate cancer aggressiveness and lethality despite biopsy-sampling error | Br. J. Cancer | 25032733 | Acquisition, processing, quality control, and annotation of FFPE prostate cancer tissue blocks. A set of FFPE human prostate cancer tissue blocks with clinical annotations and long-term patient outcome information was acquired from Folio Biosciences | Full Paper |
2014 | Hermetz, KE;Newman, S;Conneely, KN;Martin, CL;Ballif, BC;Shaffer, LG;Cody, JD;Rudd, MK; | Large inverted duplications in the human genome form via a fold-back mechanism | PLoS Genet. | 24497845 | SureSelect capture and Illumina HiSeq sequencing were performed at Hudson Alpha Genomic Services Lab (http://www.hudsonalpha.org/gsl/). After NGS, we aligned 100-bp paired-end reads from fastq files to the GRC37/hg19 reference genome using Burrows-Wh | Full Paper |
2014 | Campbell, VT;Nadesan, P;Ali, SA;Wang, CY;Whetstone, H;Poon, R;Wei, Q;Keilty, J;Proctor, J;Wang, LW;Apte, SS;McGovern, K;Alman, BA;Wunder, JS; | Hedgehog pathway inhibition in chondrosarcoma using the smoothened inhibitor IPI-926 directly inhibits sarcoma cell growth | Mol. Cancer Ther. | 24634412 | Formalin-fixed, paraffin-embedded human chondrosarcoma tissue microarrays (TMA) were purchased from Folio Biosciences for cilia staining. Before staining, all slides were placed in a 60 C oven and baked for 1 hour. Sections were deparaffinized in xyl | Full Paper |
2014 | Folcik, VA;Garofalo, M;Coleman, J;Donegan, JJ;Rabbani, E;Suster, S;Nuovo, A;Magro, CM;Di Leva, G;Nuovo, GJ; | Idiopathic pulmonary fibrosis is strongly associated with productive infection by herpesvirus saimiri | Mod. Pathol. | 24232864 | 1] The Ohio State University (OSU) at Marion, Marion, OH, USA [2] OSU Computer Science and Engineering, Marion, OH, USA [3] OSU Innovation Group for the Study of Complexity in Human, Natural, and Engineered Systems, Marion, OH, USA.++Satellite Labora | Full Paper |
2014 | Lee, CW;Zhan, Q;Lezcano, C;Frank, MH;Huang, J;Larson, AR;Lin, JY;Wan, MT;Lin, PI;Ma, J;Kleffel, S;Schatton, T;Lian, CG;Murphy, GF; | Nestin depletion induces melanoma matrix metalloproteinases and invasion | Lab. Invest. | 25365206 | An additional 153 cases of primary and metastatic melanomas demonstrating clear nodular or infiltrative growth patterns were evaluated for nestin expression in melanoma tissue microarrays (TMA) purchased from Folio Bio (Powell, OH, USA), US Biomax (R | Full Paper |
2014 | Johansson, H;Orjalo, A;Coassin, S;Yin, R; | Abstract 3399: Detection and validation of novel RNA cancer biomarkers by single molecule RNA fluorescence in situ hybridization (smRNA FISH) | Molecular and Cellular Biology | Methods Mol Biol. Ron Cook - Founder, President and CEO Marc Beal - Director of Corporate Development Peter Coassin - Director of Advanced Technology Development Entire Stellaris Team OPKOLab Folio Biosciences #3399 A B A B SK-BR-3 MCF7 T47D | Full Paper | |
2014 | Hornby, PJ;Cooper, PR;Kliwinski, C;Ragwan, E;Mabus, JR;Harman, B;Thompson, S;Kauffman, AL;Yan, Z;Tam, SH;Dorai, H;Powers, GD;Giles-Komar, J; | Human and non-human primate intestinal FcRn expression and immunoglobulin G transcytosis | Pharm. Res. | 24072267 | Paraffin-imbedded 5 μm sections were cut from full-thickness intestine harvested from monkey and human donors, and from human tissue bank (MTB-311 and MTB-316; Qualtek Molecular Labs, Goleta, CA) and dried on glass slides (60°C). Steam heat induced e | Full Paper |
2014 | Jane, EP;Premkumar, DR;Morales, A;Foster, KA;Pollack, IF; | Inhibition of phosphatidylinositol 3-kinase/AKT signaling by NVP-BKM120 promotes ABT-737-induced toxicity in a caspase-dependent manner through mitochondrial dysfunction and DNA damage response in established and primary cultured glioblastoma cells | J. Pharmacol. Exp. Ther. | 24741074 | The human glioma cell lines U87 (PTEN deleted, p53 wild type) and T98G (PTEN mutant, p53 mutant) were purchased from the ATCC (Manassas, VA). LN18 (PTEN wild type, p53 mutant), LN229 (PTEN wild type, p53 mutant), and LNZ308 (PTEN deleted, p53 deleted | Full Paper |
2014 | Hassan, F;Xu, X;Nuovo, G;Killilea, DW;Tyrrell, J;Da Tan, C;Tarran, R;Diaz, P;Jee, J;Knoell, D;Boyaka, PN;Cormet-Boyaka, E; | Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels | Respir. Res. | 24957904 | ... ell@osumc.edu Boyaka Prosper N 1 boyaka.1@osu.edu Cormet-Boyaka Estelle 1 boyaka.2@osu.edu 1Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA 2Phylogeny Inc., Columbus, OH, USA 3Nutrition a | Full Paper |
2014 | Burger, KL;Learman, BS;Boucherle, AK;Sirintrapun, SJ;Isom, S;Díaz, B;Courtneidge, SA;Seals, DF; | Src-dependent Tks5 phosphorylation regulates invadopodia-associated invasion in prostate cancer cells | Prostate | 24174371 | To conduct an immunohistochemical analysis of Tks5 protein, we purchased a high density prostate tumor microarray (Folio Bioscience, Powell, OH) containing 92, duplicate cases of formalin‐fixed human prostatic adenocarcinoma and 20 cases of normal an | Full Paper |
2014 | Gómez-Díaz, E;Rivero, A;Chandre, F;Corces, VG; | Insights into the epigenomic landscape of the human malaria vector Anopheles gambiae | Front Genet | 25177345 | Total RNA was extracted from fresh mosquito tissues (~25 midguts and ~ 50 salivary glands) using the mirVana™ RNA Isolation Kit (Ambion®) according to the manufacturer's protocol. RNA concentration was quantified using a Qubit® 2.0 Fluorometer, and R | Full Paper |
2014 | Xu, J;Zhao, Z;Zhang, X;Zheng, X;Li, J;Jiang, Y;Kuang, Y;Zhang, Y;Feng, J;Li, C;Yu, J;Li, Q;Zhu, Y;Liu, Y;Xu, P;Sun, X; | Development and evaluation of the first high-throughput SNP array for common carp (Cyprinus carpio) | BMC Genomics | 24762296 | ;... es were collected and DNA was extracted using a DNeasy Blood & Tissue Kit (Qiagen, Shanghai, China). The samples are listed in Table 1. DNA library preparation and sequencing were carried out at the HudsonAlpha Genomic Services Laboratory (Hunts | Full Paper |
2014 | Gruver, AM;Liu, L;Vaillancourt, P;Yan, SC;Cook, JD;Roseberry Baker, JA;Felke, EM;Lacy, ME;Marchal, CC;Szpurka, H;Holzer, TR;Rhoads, EK;Zeng, W;Wortinger, MA;Lu, J;Chow, CK;Denning, IJ;Beuerlein, G;Davies, J;Hanson, JC;Credille, KM;Wijayawardana, SR;Schade, AE; | Immunohistochemical application of a highly sensitive and specific murine monoclonal antibody recognising the extracellular domain of the human hepatocyte growth factor receptor (MET) | Histopathology | 25039923 | both acquired from US Biomax, Rockville, MD, USA), and 67 NSCLC whole‐tissue specimens purchased from Asterand (Detroit, MI, USA), US Biomax, Capital Biosciences, Cybridi (Gaithersburg, MD, USA), Indivumed (Baltimore, MD, USA), Conversant Bio (Huntsv | Full Paper |
2014 | Li, C;Beck, BH;Peatman, E; | Nutritional impacts on gene expression in the surface mucosa of blue catfish (Ictalurus furcatus) | Dev. Comp. Immunol. | 24378224 | ; For each timepoint, equal amounts of RNA from skin and gill were pooled together to generate 3 pooled replicates for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Hun | Full Paper |
2014 | Ehrhardt, N;Bedoya, C;Péterfy, M; | Embryonic viability, lipase deficiency, hypertriglyceridemia and neonatal lethality in a novel LMF1-deficient mouse model | Nutr Metab (Lond) | 25302068 | RT-PCR. p6. CGTGGAGCTTCTTGTGCCT. qPCR. p7. GGATCTGCAGCACTCCAT. qPCR. 36B4-f. CACTGGTCTAGGACCCGAGAAG. qPCR. 36B4-r. GGTGCCTCTGGAGATTTTCG. qPCR. RNA analysis. In situ hybridization was carried out by Phylogeny Inc. as described [11] | Full Paper |
2014 | Choudhary, A;Mambo, E;Sanford, T;Boedigheimer, M;Twomey, B;Califano, J;Hadd, A;Oliner, KS;Beaudenon, S;Latham, GJ;Adai, AT; | Evaluation of an integrated clinical workflow for targeted next-generation sequencing of low-quality tumor DNA using a 51-gene enrichment panel | BMC Med Genomics | 25395014 | The 26 tumor specimens were all colon or rectal adenocarcinomas obtained from Asterand (Detroit, MI, USA). The remaining colon cancer FFPE tissue specimens come from stage II tumors that were acquired from FolioBio (Columbus, OH) | Full Paper |
2014 | Stavropoulos, SW;Sing, RF;Elmasri, F;Silver, MJ;Powell, A;Lynch, FC;Aal, AK;Lansky, AJ;Settlage, RA;Muhs, BE;, ; | The DENALI Trial: an interim analysis of a prospective, multicenter study of the Denali retrievable inferior vena cava filter | J Vasc Interv Radiol | 25066514 | Full Paper | |
2014 | Day, K;Song, J;Absher, D; | Targeted sequencing of large genomic regions with CATCH-Seq | PLoS ONE | 25357200 | HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United States of America.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United States of America.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United Sta | Full Paper |
2014 | Sun, L;Liu, S;Wang, R;Jiang, Y;Zhang, Y;Zhang, J;Bao, L;Kaltenboeck, L;Dunham, R;Waldbieser, G;Liu, Z; | Identification and analysis of genome-wide SNPs provide insight into signatures of selection and domestication in channel catfish (Ictalurus punctatus) | PLoS ONE | 25313648 | ; protocol. Equal amounts of DNA (100 µg) from each individual were pooled for sequencing, one pool for each strain. Sequencing was conducted commercially at HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). Genomic | Full Paper |
2014 | Jiang, Y;Zhang, S;Xu, J;Feng, J;Mahboob, S;Al-Ghanim, KA;Sun, X;Xu, P; | Comparative transcriptome analysis reveals the genetic basis of skin color variation in common carp | PLoS ONE | 25255374 | ;... nufacturer’s instructions. RNA quality was verified using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Equal amounts of high-quality RNA from each tissue were pooled and sent to HudsonAlpha Genomic Services Laboratory (Huntsv | Full Paper |
2014 | Jun, LS;Showalter, AD;Ali, N;Dai, F;Ma, W;Coskun, T;Ficorilli, JV;Wheeler, MB;Michael, MD;Sloop, KW; | A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptor | PLoS ONE | 24695667 | For IHC, tissues and cells were fixed and processed for paraffin embedding using routine procedures as described previously [30]. Paraffin-embedded human pancreas samples were purchased from Folio Biosciences (Columbus, OH). IP-WB and mRNA Expression | Full Paper |
2014 | Luo, Y;Li, C;Landis, AG;Wang, G;Stoeckel, J;Peatman, E; | Transcriptomic profiling of differential responses to drought in two freshwater mussel species, the giant floater Pyganodon grandis and the pondhorn Uniomerus tetralasmus | PLoS ONE | 24586812 | ; construction, Illumina sequencing and expression analysis. RNA-Seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were constructed with | Full Paper |
2014 | Li, SZ;Hu, YY;Zhao, J;Zhao, YB;Sun, JD;Yang, YF;Ji, CC;Liu, ZB;Cao, WD;Qu, Y;Liu, WP;Cheng, G;Fei, Z; | MicroRNA-34a induces apoptosis in the human glioma cell line, A172, through enhanced ROS production and NOX2 expression | Biochem. Biophys. Res. Commun. | 24393844 | 2. Materials and methods. 2.1. Human samples and cell lines. Human glioma cancer tissue microarrays (Lot Number: ARY-HH0118-011-02) containing 60 glioma cancer specimens and 30 normal tissues were purchased from Folio Biosciences (Columbus, OH, USA) | Full Paper |
2014 | Yim, KM;Brewer, MS;Miller, CT;Gillespie, RG; | Comparative transcriptomics of maturity-associated color change in Hawaiian spiders | J. Hered. | 25149253 | Extractions were shipped to the Genomic Services Lab at HudsonAlpha (Huntsville, AL) for library preparation and sequencing. Complementary DNA libraries were prepared from total RNA, and 50bp paired-end Illumina HiSeq 2000 RNA-seq was used to generat | Full Paper |
2014 | Shipitsin, M;Small, C;Giladi, E;Siddiqui, S;Choudhury, S;Hussain, S;Huang, YE;Chang, H;Rimm, DL;Berman, DM;Nifong, TP;Blume-Jensen, P; | Automated quantitative multiplex immunofluorescence in situ imaging identifies phospho-S6 and phospho-PRAS40 as predictive protein biomarkers for prostate cancer lethality | Proteome Sci | 25075204 | In total, we procured and tested 508 unique prostatectomy samples with lethal outcome annotation available (Folio Biosciences, Powell, OH). Of these, 418 passed quality testing and were used for our TMA (Additional file 1: Table S2). Figure 2 Figure | Full Paper |
2014 | Drogemöller, BI;Niehaus, DJ;Chiliza, B;van der Merwe, L;Asmal, L;Malhotra, AK;Wright, GE;Emsley, R;Warnich, L; | Patterns of variation influencing antipsychotic treatment outcomes in South African first-episode schizophrenia patients | Pharmacogenomics | 24444409 | gDNA from the 11 individuals was extracted from venous blood using the QIAamp DNA Blood Maxi Kit (Qiagen, Germany), accord - ing to the manufacturer’s instructions. There - after, the gDNA samples were sent to the HudsonAlpha Genomics Service Labora | Full Paper |
2014 | Zhang, J;Vernes, JM;Ni, J;Nelson, C;Wong, A;Chen, ST;Asundi, A;Vandlen, R;Meng, YG; | Real-time immuno-polymerase chain reaction in a 384-well format: detection of vascular endothelial growth factor and epidermal growth factor-like domain 7 | Anal. Biochem. | 25013989 | CAL-12T cell line was purchased from German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). Human cancer tissues were purchased from ConversantBio (Huntsville, AL) or Avaden BioSciences (Scarsdale, NY) | Full Paper |
2014 | Aycock, KI;Campbell, RL;Manning, KB;Sastry, SP;Shontz, SM;Lynch, FC;Craven, BA; | A computational method for predicting inferior vena cava filter performance on a patient-specific basis | J Biomech Eng | 24805200 | Full Paper | |
2014 | Sarran-Armogan, L; | Prospective isolation of human myeloerythroid progenitors in fetal liver and embryonic stem cell differentiation cultures | Thesis | Einstein Fetal Tissue repository under IRB approved protocol. Human cord blood was obtained from the Obstetric Department at the Weiler Hospital of the Albert Einstein College of Medicine under an IRB approved protocol. Human bone marrow CD34+ cells | Full Paper | |
2014 | Miyake, M;Lawton, A;Goodison, S;Urquidi, V;Rosser, CJ; | Chemokine (C-X-C motif) ligand 1 (CXCL1) protein expression is increased in high-grade prostate cancer | Pathol. Res. Pract. | 24252309 | High-density commercial tissue microarrays (TMA) (ARY-HH0185, Foliobio, Powell, OH) constructed from formalin fixed, paraffin embedded clinical samples comprised the cohort of 110 patients (90 prostate cancer and 20 benign prostates without evidence | Full Paper |
2014 | Pollen, AA;Nowakowski, TJ;Shuga, J;Wang, X;Leyrat, AA;Lui, JH;Li, N;Szpankowski, L;Fowler, B;Chen, P;Ramalingam, N;Sun, G;Thu, M;Norris, M;Lebofsky, R;Toppani, D;Kemp, DW;Wong, M;Clerkson, B;Jones, BN;Wu, S;Knutsson, L;Alvarado, B;Wang, J;Weaver, LS;May, AP;Jones, RC;Unger, MA;Kriegstein, AR;West, JA; | Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex | Nature biotechnology | 25086649 | For systems verification tests of capture efficiency, primary cells were obtained from splenocytes (AllCells, LLC PB003F) and peripheral blood mononuclear cells (AllCells, LLC PB003F) Save | Full Paper |
2014 | Cohen, A; | Regenerative potential of human CD34+ stem cells mobilized and enriched from bone marrow | Thesis | selected from granulocyte colony-stimulating factor (G-CSF) (Amgen; Thousand Oaks, CA, USA) mobilized peripheral blood mononuclear cells (mPBMNCs) obtained from AllCells (Regimen E, Emeryville, CA). Healthy human donors were injected with G-CSF once | Full Paper | |
2014 | Koellmer, M; | Multilineage differentiation of mesenchymal stem cells for cell-based tissue engineering | Thesis | Human bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque TM PLUS solution and RosetteSep® Human Mesenchymal Stem Cell Enrichment Cocktail (Stem Cell Technolog | Full Paper | |
2014 | Lu, M;Xia, L;Li, Y;Wang, X;Hoffman, R; | The orally bioavailable MDM2 antagonist RG7112 and pegylated interferon α 2a target JAK2V617F-positive progenitor and stem cells | Blood | 24869939 | human CD34+ cell selection kit (StemCell Technologies, Vancouver, BC, Canada). Normal human bone marrow (BM) mononuclear cells and CD34+ cells were purchased from AllCells (Emeryville, CA). The experiments for which the CD34+ cells from each of the 3 | Full Paper |
2014 | Shi, J;Rose, EL;Singh, A;Hussain, S;Stagliano, NE;Parry, GC;Panicker, S; | TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutinins | Blood | 24695853 | Human RBCs (hRBCs; blood type O negative; Allcells, Alameda, CA) were washed 3 times with gelatin veronal buffer (GVB; Complement Technology, Tyler, TX) and resuspended at 1 × 109 cells per milliliter in GVB plus 10 mM EDTA. To sensitize hRBCs with C | Full Paper |
2014 | Adamia, S;Bar-Natan, M;Haibe-Kains, B;Pilarski, PM;Bach, C;Pevzner, S;Calimeri, T;Avet-Loiseau, H;Lode, L;Verselis, S;Fox, EA;Galinsky, I;Mathews, S;Dagogo-Jack, I;Wadleigh, M;Steensma, DP;Motyckova, G;Deangelo, DJ;Quackenbush, J;Tenen, DG;Stone, RM;Griffin, JD; | NOTCH2 and FLT3 gene mis-splicings are common events in patients with acute myeloid leukemia (AML): new potential targets in AML | Blood | 24574459 | Our previous studies revealed an increase in alternative splicing of multiple RNAs in cells from patients with acute myeloid leukemia (AML) compared with CD34 + bone marrow cells Save | Full Paper |
2014 | Krow-Lucal, ER;Kim, CC;Burt, TD;McCune, JM; | Distinct functional programming of human fetal and adult monocytes | Blood | 24518760 | Adult bone marrow samples were obtained from healthy donors (AllCells and Lonza Group). Both adult and fetal mononuclear cells were isolated by density centrifugation of a Ficoll- Save | Full Paper |
2014 | Shi, Q;Kuether, EL;Chen, Y;Schroeder, JA;Fahs, SA;Montgomery, RR; | Platelet gene therapy corrects the hemophilic phenotype in immunocompromised hemophilia A mice transplanted with genetically manipulated human cord blood stem cells | Blood | 24269957 | Human cord blood (hCB) CD34+ cells were purchased from AllCells (Emeryville, CA) and transduced with 2bF8LV by using a protocol similar to that described in previous reports.10,15 The details are provided in the supplemental Data (available on the Bl | Full Paper |
2014 | Thompson, A;Rasko, J;Hongeng, S;Kwiatkowski, J;Schiller, G;von Kalle, C;Cavazzana, M;Leboulch, P;Petrusich, A;Soni, S;Walters, M; | Initial Results from the Northstar Study (HGB-204): A Phase 1/2 Study of Gene Therapy for β-Thalassemia Major Via Transplantation of Autologous Hematopoietic Stem Cells Transduced Ex Vivo with a Lentiviral βΑ-T87Q -Globin Vector (LentiGlobin BB305 Drug Product) | Blood | Background: Hematopoietic stem cell (HSC) gene therapy has the potential to induce globin production and mitigate the need for blood transfusions in β-thalassemia major. Promising early results for 2 subjects with β0/βE -thalassemia major in the ongo | Full Paper | |
2014 | Iwasa, M;Miura, Y;Fujishiro, A;Tamura, A;Sato, A;Yokota, A;Nakagawa, Y;Yoshioka, S;Hirai, H;Andoh, A;Ichinohe, T;Maekawa, T; | Effects of Irradiation on the Functional Characteristics of Human Bone Marrow Mesenchymal Stromal/Stem Cells | Blood | BM samples were purchased from AllCells (Emeryville, CA). For the irradiation experiments, BM-MSCs were g-irradiated (Cesium-137) with various doses ranged from 2 to 12 Gy by a Save;Total-body irradiation is frequently used as a conditioning treatm | Full Paper | |
2014 | Sahler, J; | Modulation of Transcription Factors in Platelets and Platelet Microparticles Attenuates Inflammatory Responses | Thesis | Adapted from previous procedures, 94 human CD34+ cord blood cells were purchased from Allcells (Emeryville, CA) and first plated at 2.5x105 cells/mL in IMDM supplemented with 20% BIT 9500, 0.8% Low Density Lipoprotein (L-8292, Sigma), 100ng/mL recomb | Full Paper | |
2014 | Li, S;Garrett-Bakelman, F;Perl, AE;Luger, SM;Zhang, C;To, BL;Lewis, ID;Brown, AL;D'Andrea, RJ;Ross, ME;Levine, R;Carroll, M;Melnick, A;Mason, CE; | Dynamic evolution of clonal epialleles revealed by methclone | Genome biology | 25260792 | samples at two time points (diagnosis and relapse stages), enriched for myeloblast cells, were used in the experiments, and these samples were also compared to five CD34+ normal bone marrow samples (AllCells, Inc.). Institutional review board approva | Full Paper |
2014 | Bartholdy, B;Christopeit, M;Will, B;Mo, Y;Barreyro, L;Yu, Y;Bhagat, TD;Okoye-Okafor, UC;Todorova, TI;Greally, JM;Levine, RL;Melnick, A;Verma, A;Steidl, U; | HSC commitment-associated epigenetic signature is prognostic in acute myeloid leukemia | The Journal of clinical investigation | 24487588 | Acute myeloid leukemia (AML) is characterized by disruption of HSC and progenitor cell differentiation. Frequently, AML is associated with mutations in genes encoding epigenetic Save | Full Paper |
2014 | Schlinker, AC; | In Vitro Platelet Production: Promoting Megakaryocyte Terminal Differentiation, Demonstrating Platelet Functionality, and Developing an Automated Process for Platelet Harvest | Thesis | Figure 4.1. PLT-like particle (PLP) and proPLT production in X-VIVO 10 cultures. (A) Blood PLTs obtained from AllCells (top panels) and PLPs exposed to shear for 3 days after CD61+ selection on day 10 (bottom panels) were stained with anti-CD41 and a | Full Paper | |
2014 | Accomando, WP;Wiencke, JK;Houseman, EA;Nelson, HH;Kelsey, KT; | Quantitative reconstruction of leukocyte subsets using DNA methylation | Genome biology | 24598480 | ummarizes the workflow carried out for all samples, derived from human whole blood, that were utilized in this work. Blood was obtained through an institutional review board-approved donor program at AllCells, LLC (Alameda, CA, USA) and research was | Full Paper |
2014 | Liu, GH;Suzuki, K;Li, M;Qu, J;Montserrat, N;Tarantino, C;Gu, Y;Yi, F;Xu, X;Zhang, W;Ruiz, S;Plongthongkum, N;Zhang, K;Masuda, S;Nivet, E;Tsunekawa, Y;Soligalla, RD;Goebl, A;Aizawa, E;Kim, NY;Kim, J;Dubova, I;Li, Y;Ren, R;Benner, C;Del Sol, A;Bueren, J;Trujillo, JP;Surralles, J;Cappelli, E;Dufour, C;Esteban, CR;Belmonte, JCI; | Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCs | Nature communications | 24999918 | Human BM CD34 + cells from a healthy individual were purchased from Allcells (Alameda, CA). FA patient BM cells were isolated from two FA patients bearing FANCA mutations ( Save | Full Paper |
2014 | Green, MR;Vicente-Dueñas, C;Romero-Camarero, I;Long Liu, C;Dai, B;González-Herrero, I;García-Ramírez, I;Alonso-Escudero, E;Iqbal, J;Chan, WC;Campos-Sanchez, E;Orfao, A;Pintado, B;Flores, T;Blanco, O;Jiménez, R;Martínez-Climent, JA;Criado, FJ;Cenador, MB;Zhao, S;Natkunam, Y;Lossos, IS;Majeti, R;Melnick, A;Cobaleda, C;Alizadeh, AA;Sánchez-García, I; | Transient expression of Bcl6 is sufficient for oncogenic function and induction of mature B-cell lymphoma | Nature communications | 24887457 | Bone marrow from two healthy donors (1 male, 1 female) were obtained purchased from AllCells, LLC. (Emeryville, CA) and sorted on a FACS ARIA II instrument as PI−, Lin−, CD34+, CD38−. Single cells were sorted into Terasaki plates containing alternati | Full Paper |
2014 | Minn, I;Wang, H;Mease, RC;Byun, Y;Yang, X;Wang, J;Leach, SD;Pomper, MG; | A red-shifted fluorescent substrate for aldehyde dehydrogenase | Nature communications | 24759454 | (BD Biosciences, San Jose, CA) with 488 nm blue laser and phycoerythrin (PE) filters. Isolation of ALDHhi human hematopoietic stem cells Frozen human cord blood mononuclear cells were purchased from ALLCELLS (Alamenda, CA). Cells were thawed accordi | Full Paper |
2014 | Verma, R;Su, S;McCrann, DJ;Green, JM;Leu, K;Young, PR;Schatz, PJ;Silva, JC;Stokes, MP;Wojchowski, DM; | RHEX, a novel regulator of human erythroid progenitor cell expansion and erythroblast development | The Journal of experimental medicine | 25092874 | O (and cytokine) challenges were at the concentrations and time intervals indicated. Primary human hematopoietic cells used in RT-PCR analyses included monocytes, neutrophils, T cells, and platelets (AllCells). Murine G1E-ER4 cells (M. Weiss, Childre | Full Paper |
2014 | Roche, ET;Hastings, CL;Lewin, SA;Shvartsman, D;Brudno, Y;Vasilyev, NV;O'Brien, FJ;Walsh, CJ;Duffy, GP;Mooney, DJ; | Comparison of biomaterial delivery vehicles for improving acute retention of stem cells in the infarcted heart | Biomaterials | 24862441 | hMSCs (AllCells, USA) were encapsulated in chitosan/β-GP or alginate gels at a density of 100,000 cells/100μL gel (1 × 106 cells/mL) and 100μL was added to a hanging well cell culture insert with a pore size of 1μm, suitable for insertion into a 48-w | Full Paper |
2014 | Adamia, S;Haibe-Kains, B;Pilarski, PM;Bar-Natan, M;Pevzner, S;Avet-Loiseau, H;Lode, L;Verselis, S;Fox, EA;Burke, J;Galinsky, I;Dagogo-Jack, I;Wadleigh, M;Steensma, DP;Motyckova, G;Deangelo, DJ;Quackenbush, J;Stone, R;Griffin, JD; | A genome-wide aberrant RNA splicing in patients with acute myeloid leukemia identifies novel potential disease markers and therapeutic targets | Clinical cancer research : an official journal of the American Association for Cancer Research | 24284058 | A control group of 12 normal donors of both genders was also included: 8 bone marrow samples of normal donors were obtained from AllCells (California) and 4 peripheral blood Save | Full Paper |
2014 | Wang, X;Moghimi, B;Zolotukhin, I;Morel, LM;Cao, O;Herzog, RW; | Immune tolerance induction to factor IX through B cell gene transfer: TLR9 signaling delineates between tolerogenic and immunogenic B cells | Molecular therapy : the journal of the American Society of Gene Therapy | 24609143 | Primary B cells derived from healthy humans were purchased from Allcells (Alameda, CA). Cell culture reagents were purchased from Invitrogen (Grand Island, NY). Recombinant Save | Full Paper |
2014 | Carbonaro, DA;Zhang, L;Jin, X;Montiel-Equihua, C;Geiger, S;Carmo, M;Cooper, A;Fairbanks, L;Kaufman, ML;Sebire, NJ;Hollis, RP;Blundell, MP;Senadheera, S;Fu, PY;Sahaghian, A;Chan, RY;Wang, X;Cornetta, K;Thrasher, AJ;Kohn, DB;Gaspar, HB; | Preclinical demonstration of lentiviral vector-mediated correction of immunological and metabolic abnormalities in models of adenosine deaminase deficiency | Molecular therapy : the journal of the American Society of Gene Therapy | 24256635 | Normal human adult bone marrow samples (100 ml/donor) were also purchased from AllCells, LLC (Emeryville, CA). Human CD34+ cell isolation was performed as described.51 Briefly, human cord blood or human bone marrow was diluted 1:2 with Dulbecco's pho | Full Paper |
2014 | Huang, X;Shah, S;Wang, J;Ye, Z;Dowey, SN;Tsang, KM;Mendelsohn, LG;Kato, GJ;Kickler, TS;Cheng, L; | Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors | Molecular therapy : the journal of the American Society of Gene Therapy | 24002691 | Johns Hopkins University Institutional Review Board. Human primary CB MNCs are obtained from anonymous, healthy donors and then isolated in our lab using Ficoll-Paque Plus (P = 1.077) or directly purchased from AllCells, LLC (AllCells, Emeryville, CA | Full Paper |
2014 | Chorzalska, A;Salloum, I;Shafqat, H;Khan, S;Marjon, P;Treaba, D;Schorl, C;Morgan, J;Bryke, CR;Falanga, V;Zhao, TC;Reagan, J;Winer, E;Olszewski, AJ;Al-Homsi, AS;Kouttab, N;Dubielecka, PM; | Low expression of Abelson interactor-1 is linked to acquired drug resistance in Bcr-Abl-induced leukemia | Leukemia | 24699303 | cross-talk may be causative in the acquired drug resistant phenotype of LSCs. Materials and methods Patients and donors Human CD34+ cells were isolated from bone marrow (BM) mononuclear cells (MNC) (AllCells, LLC, Emeryville, CA) or from peripheral | Full Paper |
2014 | Yang, H;Bueso-Ramos, C;DiNardo, C;Estecio, MR;Davanlou, M;Geng, QR;Fang, Z;Nguyen, M;Pierce, S;Wei, Y;Parmar, S;Cortes, J;Kantarjian, H;Garcia-Manero, G; | Expression of PD-L1, PD-L2, PD-1 and CTLA4 in myelodysplastic syndromes is enhanced by treatment with hypomethylating agents | Leukemia | 24270737 | from bone marrow specimens using CD34+ MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s protocol. Bone marrow samples from 4 healthy donors were obtained from Allcells (Emeryville, CA). Peripheral blood (PB) sam | Full Paper |
2014 | Kharabi Masouleh, B;Geng, H;Hurtz, C;Chan, LN;Logan, AC;Chang, MS;Huang, C;Swaminathan, S;Sun, H;Paietta, E;Melnick, AM;Koeffler, P;Müschen, M; | Mechanistic rationale for targeting the unfolded protein response in pre-B acute lymphoblastic leukemia | Proceedings of the National Academy of Sciences of the United States of America | 24821775 | For magnetic bead sorting, peripheral blood or bone marrow mononuclear cells were purchased from AllCells LLC and CD19-enrichment was performed by magnetic bead cell sorting Save | Full Paper |
2014 | Murray, JC;Aldeghaither, D;Wang, S;Nasto, RE;Jablonski, SA;Tang, Y;Weiner, LM; | c-Abl modulates tumor cell sensitivity to antibody-dependent cellular cytotoxicity | Cancer immunology research | 25300860 | Frozen primary peripheral blood mononuclear cells (PBMC) from three individual donors (AllCells) were enriched for NK cells (Human NK Cell Enrichment Kit; STEMCELL Technologies Save | Full Paper |
2014 | Wang, Y;Yi, S;Sun, L;Huang, Y;Zhang, M; | Charge-selective fractions of naturally occurring nanoparticles as bioactive nanocarriers for cancer therapy | Acta biomaterialia | 24952072 | Splenocytes, derived from C57BL/6 mice, were purchased from the Allcells Company (Emeryville, CA). HEPES, 1,9-dimethyl-methylene blue (DMMB), chondroitin sulfate (CS), Sephadex G75, DEAE-cellulose, and phosphate-buffered saline (PBS) were purchased f | Full Paper |
2014 | Fu, Y;Yip, A;Seah, PG;Blasco, F;Shi, PY;Hervé, M; | Modulation of inflammation and pathology during dengue virus infection by p38 MAPK inhibitor SB203580 | Antiviral research | 25131378 | Dengue virus (DENV) infection could lead to dengue fever (DF), dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). The disease outcome is controlled by both viral Save | Full Paper |
2014 | Rampal, R;Alkalin, A;Madzo, J;Vasanthakumar, A;Pronier, E;Patel, J;Li, Y;Ahn, J;Abdel-Wahab, O;Shih, A;Lu, C;Ward, PS;Tsai, JJ;Hricik, T;Tosello, V;Tallman, JE;Zhao, X;Daniels, D;Dai, Q;Ciminio, L;Aifantis, I;He, C;Fuks, F;Tallman, MS;Ferrando, A;Nimer, S;Paietta, E;Thompson, CB;Licht, JD;Mason, CE;Godley, LA;Melnick, A;Figueroa, ME;Levine, RL; | DNA hydroxymethylation profiling reveals that WT1 mutations result in loss of TET2 function in acute myeloid leukemia | Cell reports | 25482556 | Eleven human CD34 + bone marrow samples were provided by the Stem Cell and Xenograft Core Facility of the University of Pennsylvania or purchased from AllCells. These studies Save | Full Paper |
2014 | Li, D;Xie, K;Ding, G;Li, J;Chen, K;Li, H;Qian, J;Jiang, C;Fang, J; | Tumor resistance to anti-VEGF therapy through up-regulation of VEGF-C expression | Cancer letters | 24333721 | Human umbilical vein endothelial cells (HUVECs) were obtained from Allcells (Emeryville, , and amphotericin-B (HUVEC-004; Allcells). All cells were authenticated according to the cell Save | Full Paper |
2014 | Li, X;Tong, LJ;Ding, J;Meng, LH; | Systematic combination screening reveals synergism between rapamycin and sunitinib against human lung cancer | Cancer letters | 24018642 | The human umbilical vein endothelial cells (HUVECs) were purchased from AllCells and maintained in M199 medium supplemented with 20% heat-inactivated FBS, penicillin (100 IU/ Save | Full Paper |
2014 | Yao, WT;Wu, JF;Yu, GY;Wang, R;Wang, K;Li, LH;Chen, P;Jiang, YN;Cheng, H;Lee, HW;Yu, J;Qi, H;Yu, XJ;Wang, P;Chu, YW;Yang, M;Hua, ZC;Ying, HQ;Hoffman, RM;Jeong, LS;Jia, LJ; | Suppression of tumor angiogenesis by targeting the protein neddylation pathway | Cell death & disease | 24525735 | HUVECs were purchased from Allcells Biotech Co. (Shanghai, China) and cultured in a complete endothelial cell medium (Allcells Biotech Co.) at 37C with 5% CO 2 . Save | Full Paper |
2014 | Li, D;Wei, X;Xie, K;Chen, K;Li, J;Fang, J; | A novel decoy receptor fusion protein for FGF-2 potently inhibits tumour growth | British journal of cancer | 24874473 | cells were purchased from AllCells (Emeryville, CA, USA) and cultured in complete HUVEC medium (AllCells) containing 10% FBS and 10% growth factor supplement (AllCells). Caki-1 Save | Full Paper |
2014 | Haselmayer, P;Camps, M;Muzerelle, M;El Bawab, S;Waltzinger, C;Bruns, L;Abla, N;Polokoff, MA;Jond-Necand, C;Gaudet, M;Benoit, A;Bertschy Meier, D;Martin, C;Gretener, D;Lombardi, MS;Grenningloh, R;Ladel, C;Petersen, JS;Gaillard, P;Ji, H; | Characterization of Novel PI3Kδ Inhibitors as Potential Therapeutics for SLE and Lupus Nephritis in Pre-Clinical Studies | Frontiers in immunology | 24904582 | terial smooth muscle cells and keratinocytes (Lonza, Inc., Allendale, NJ, USA) were as previously described. Positively selected primary normal human CD19+ B cells and CD4+ T-cells were obtained from AllCells (Emeryville, CA, USA). PBMC were isolated | Full Paper |
2014 | Fu, J;Tjandra, M;Becker, C;Bednarczyk, D;Capparelli, M;Elling, R;Hanna, I;Fujimoto, R;Furegati, M;Karur, S;Kasprzyk, T;Knapp, M;Leung, K;Li, X;Lu, P;Mergo, W;Miault, C;Ng, S;Parker, D;Peng, Y;Roggo, S;Rivkin, A;Simmons, RL;Wang, M;Wiedmann, B;Weiss, AH;Xiao, L;Xie, L;Xu, W;Yifru, A;Yang, S;Zhou, B;Sweeney, ZK; | Potent nonimmunosuppressive cyclophilin inhibitors with improved pharmaceutical properties and decreased transporter inhibition | Journal of medicinal chemistry | 25310383 | PBMCs of two human donors were obtained on the day of the experiment (AllCells, LLC), pelleted in their original 50 mL conical tubes at 1200 rpm for 10 min, and resuspended in 25 Save | Full Paper |
2014 | Gandhi, AK;Mendy, D;Waldman, M;Chen, G;Rychak, E;Miller, K;Gaidarova, S;Ren, Y;Wang, M;Breider, M;Carmel, G;Mahmoudi, A;Jackson, P;Abbasian, M;Cathers, BE;Schafer, PH;Daniel, TO;Lopez-Girona, A;Thakurta, A;Chopra, R; | Measuring cereblon as a biomarker of response or resistance to lenalidomide and pomalidomide requires use of standardized reagents and understanding of gene complexity | British journal of haematology | 24206017 | here, H929 lenalidomide-resistant cells and DF15R pomalidomide-resistant cells were taken out of culture with compounds for 5-7 d before use. Select primary CD138+ MM cells were purchased from AllCells (Emeryville, CA, USA). Production of recombinant | Full Paper |
2014 | Sully, EK;Malachowa, N;Elmore, BO;Alexander, SM;Femling, JK;Gray, BM;DeLeo, FR;Otto, M;Cheung, AL;Edwards, BS;Sklar, LA;Horswill, AR;Hall, PR;Gresham, HD; | Selective chemical inhibition of agr quorum sensing in Staphylococcus aureus promotes host defense with minimal impact on resistance | PLoS pathogens | 24945495 | Human neutrophils were purchased from AllCells and the source of the neutrophils was Human neutrophils (AllCells) were washed twice in saline to remove EDTA, suspended in RPMI Save | Full Paper |
2014 | Bellayr, IH;Catalano, JG;Lababidi, S;Yang, AX;Lo Surdo, JL;Bauer, SR;Puri, RK; | Gene markers of cellular aging in human multipotent stromal cells in culture | Stem cell research & therapy | 24780490 | Human MSCs from six different donors were purchased from ALLCELLS and Lonza (Table 1). Because these cells were commercially available, no patient consent or approval from the Save | Full Paper |
2014 | Wei, DG;Chiang, V;Fyne, E;Balakrishnan, M;Barnes, T;Graupe, M;Hesselgesser, J;Irrinki, A;Murry, JP;Stepan, G;Stray, KM;Tsai, A;Yu, H;Spindler, J;Kearney, M;Spina, CA;McMahon, D;Lalezari, J;Sloan, D;Mellors, J;Geleziunas, R;Cihlar, T; | Histone deacetylase inhibitor romidepsin induces HIV expression in CD4 T cells from patients on suppressive antiretroviral therapy at concentrations achieved by clinical dosing | PLoS pathogens | 24722454 | ofectamine 2000 (Life Technologies, Grand Island, NY). In vitro model of HIV latency Total peripheral blood mononuclear cells (PBMCs) were obtained from healthy HIV-negative donors by leukapheresis (AllCells, Inc, Emeryville, CA). Naive CD4+ T cells | Full Paper |
2014 | Jajosky, AN;Coad, JE;Vos, JA;Martin, KH;Senft, JR;Wenger, SL;Gibson, LF; | RepSox slows decay of CD34+ acute myeloid leukemia cells and decreases T cell immunoglobulin mucin-3 expression | Stem cells translational medicine | 24855276 | CD34 + cells isolated from the mobilized peripheral blood of healthy donors were purchased from AllCells (Emeryville, CA, http://www.allcells.com). Save | Full Paper |
2014 | Ji, J;Judkowski, VA;Liu, G;Wang, H;Bunying, A;Li, Z;Xu, M;Bender, J;Pinilla, C;Yu, JS; | Identification of novel human leukocyte antigen-A*0201-restricted, cytotoxic T lymphocyte epitopes on CD133 for cancer stem cell immunotherapy | Stem cells translational medicine | 24375541 | bone marrow cells were purchased directly from AllCells Inc. (Alameda, CA, http://www. ) cells (CD133+ and CD133‐ fractions; AllCells) from a healthy donor were included as controls. Save | Full Paper |
2014 | Wang, X;Kimbrel, EA;Ijichi, K;Paul, D;Lazorchak, AS;Chu, J;Kouris, NA;Yavanian, GJ;Lu, SJ;Pachter, JS;Crocker, SJ;Lanza, R;Xu, RH; | Human ESC-derived MSCs outperform bone marrow MSCs in the treatment of an EAE model of multiple sclerosis | Stem cell reports | 25068126 | BM-MSC lines #1, #2 and #3 were derived from frozen BM mononuclear cells (BMMNC) from 3 independent human donors and BM-MSC lines #7 and #8 were derived from fresh whole bone marrow aspirates from 2 independent donors from AllCells, Inc. BM-MSC line | Full Paper |
2014 | Karjalainen, K;Pasqualini, R;Cortes, JE;Kornblau, SM;Lichtiger, B;O'Brien, S;Kantarjian, HM;Sidman, RL;Arap, W;Koivunen, E; | Design, development, and validation of a high-throughput drug-screening assay for targeting of human leukemia | Cancer | 24496871 | negative against blood-transmittable diseases. These samples were stored at 4°C for 24 hours before use. Heparin was used as an anticoagulant. Pretested whole blood and bone marrow samples also were obtained from commercial sources (Innovative Resear | Full Paper |
2014 | Polakowski, N;Terol, M;Hoang, K;Nash, I;Laverdure, S;Gazon, H;Belrose, G;Mesnard, JM;Césaire, R;Péloponèse, JM;Lemasson, I; | HBZ stimulates brain-derived neurotrophic factor/TrkB autocrine/paracrine signaling to promote survival of human T-cell leukemia virus type 1-Infected T cells | Journal of virology | 25210182 | RNA from healthy donor CD4 T cells was purchased from AllCells. cDNA was synthesized Real-time PCR amplification of cDNAs from cell lines and cDNA purchased from AllCells was Save | Full Paper |
2014 | Lucera, MB;Tilton, CA;Mao, H;Dobrowolski, C;Tabler, CO;Haqqani, AA;Karn, J;Tilton, JC; | The histone deacetylase inhibitor vorinostat (SAHA) increases the susceptibility of uninfected CD4+ T cells to HIV by increasing the kinetics and efficiency of postentry viral events | Journal of virology | 25008921 | CD4+ T cells were obtained by leukopheresis from ALLCELLS, LLC. All subjects were 151 Save;CD4 T cells were obtained by leukapheresis from Allcells, LLC. All subjects were healthy control donors negative for hepatitis B virus (HBV), hepatitis C vir | Full Paper |
2014 | Tabler, CO;Lucera, MB;Haqqani, AA;McDonald, DJ;Migueles, SA;Connors, M;Tilton, JC; | CD4+ memory stem cells are infected by HIV-1 in a manner regulated in part by SAMHD1 expression | Journal of virology | 24554663 | Samples from healthy donors were deidentified and were obtained by leukapheresis from AllCells, LLC. HIV-infected patients were recruited from the Clinical Research Center, NIH ( Save | Full Paper |
2014 | Chen, YL;Abdul Ghafar, N;Karuna, R;Fu, Y;Lim, SP;Schul, W;Gu, F;Herve, M;Yokohama, F;Wang, G;Cerny, D;Fink, K;Blasco, F;Shi, PY; | Activation of peripheral blood mononuclear cells by dengue virus infection depotentiates balapiravir | Journal of virology | 24257621 | Antiviral assays. Cryopreserved human PBMCs were purchased from AllCells, LLC (Alameda, California). Written consent from the donors was available for all samples. All experiments involving human PBMCs were approved by the Institutional Review Board | Full Paper |
2014 | Raval, AN;Schmuck, EG;Tefera, G;Leitzke, C;Ark, CV;Hei, D;Centanni, JM;de Silva, R;Koch, J;Chappell, RG;Hematti, P; | Bilateral administration of autologous CD133+ cells in ambulatory patients with refractory critical limb ischemia: lessons learned from a pilot randomized, double-blind, placebo-controlled trial | Cytotherapy | 25239491 | Purity and viability studies were also performed on mobilized leukapheresis products obtained from healthy normal volunteers (AllCells, Almeda, CA, USA). The dose of G-CSF for Save | Full Paper |
2014 | Hulspas, R;Villa-Komaroff, L;Koksal, E;Etienne, K;Rogers, P;Tuttle, M;Korsgren, O;Sharpe, JC;Berglund, D; | Purification of regulatory T cells with the use of a fully enclosed high-speed microfluidic system | Cytotherapy | 25065635 | kit (Stem Cell Technologies Inc, Vancouver, BC, Canada) from a total of 450 million fresh human peripheral blood mononucleated cells obtained from apheresis leuko paks (AllCells Save | Full Paper |
2014 | Dasmahapatra, G;Patel, H;Friedberg, J;Quayle, SN;Jones, SS;Grant, S; | In vitro and in vivo interactions between the HDAC6 inhibitor ricolinostat (ACY1215) and the irreversible proteasome inhibitor carfilzomib in non-Hodgkin lymphoma cells | Molecular cancer therapeutics | 25239935 | Interactions between the HDAC6 inhibitor ricolinostat (ACY1215) and the irreversible proteasome inhibitor carfilzomib were examined in non-Hodgkin lymphoma (NHL) models, Save | Full Paper |
2014 | Feng, JY;Cheng, G;Perry, J;Barauskas, O;Xu, Y;Fenaux, M;Eng, S;Tirunagari, N;Peng, B;Yu, M;Tian, Y;Lee, YJ;Stepan, G;Lagpacan, LL;Jin, D;Hung, M;Ku, KS;Han, B;Kitrinos, K;Perron, M;Birkus, G;Wong, KA;Zhong, W;Kim, CU;Carey, A;Cho, A;Ray, AS; | Inhibition of hepatitis C virus replication by GS-6620, a potent C-nucleoside monophosphate prodrug | Antimicrobial agents and chemotherapy | 24419349 | Human primary bone marrow (BM) light-density cells from three different lots were obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD), and the cytotoxicity studies were Save | Full Paper |
2014 | Yao, H;Miura, Y;Yoshioka, S;Miura, M;Hayashi, Y;Tamura, A;Iwasa, M;Sato, A;Hishita, T;Higashi, Y;Kaneko, H;Ashihara, E;Ichinohe, T;Hirai, H;Maekawa, T; | Parathyroid hormone enhances hematopoietic expansion via upregulation of cadherin-11 in bone marrow mesenchymal stromal cells | Stem cells (Dayton, Ohio) | 24648356 | Parathyroid hormone (PTH) stimulates hematopoiesis in mouse models. The involvement of osteoblasts in this process has been well investigated; however, the effects of PTH on Save | Full Paper |
2014 | Pandya, J;Ortiz, L;Ling, C;Rivers, AE;Aslanidi, G; | Rationally designed capsid and transgene cassette of AAV6 vectors for dendritic cell-based cancer immunotherapy | Immunology and cell biology | 24217810 | Leukapheresis-derived peripheral blood mononuclear cells (AllCells, Alameda, CA, USA) were purified on Ficoll-Paque (GE Healthcare, Piscataway, NJ, USA). Semi-adherent cell fractions were incubated in serum-free X-VIVO medium (Lonza, Allendale, NJ, U | Full Paper |
2014 | Yoshioka, S;Miura, Y;Yao, H;Satake, S;Hayashi, Y;Tamura, A;Hishita, T;Ichinohe, T;Hirai, H;Takaor-Kondo, A;Maekawa, T; | CCAAT/enhancer-binding protein β expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesis | Stem cells (Dayton, Ohio) | 24115241 | The transcription factor CCAAT/enhancer-binding protein β (C/EBPβ) regulates the differentiation of a variety of cell types. Here, the role of C/EBPβ expressed by bone marrow Save | Full Paper |
2014 | Brunckhorst, MK;Xu, Y;Lu, R;Yu, Q; | Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironment | The American journal of pathology | 25043619 | Despite decades of research, the survival rate of ovarian cancer patients is largely unchanged. Current chemotherapeutic drugs are effective only transiently because patients with Save | Full Paper |
2014 | Bowers, PM;Verdino, P;Wang, Z;da Silva Correia, J;Chhoa, M;Macondray, G;Do, M;Neben, TY;Horlick, RA;Stanfield, RL;Wilson, IA;King, DJ; | Nucleotide insertions and deletions complement point mutations to massively expand the diversity created by somatic hypermutation of antibodies | The Journal of biological chemistry | 25320089 | Sequencing and Preparation of PBMC cDNA RNA from peripheral blood lymphocytes (PBMCs) from a total of 68 healthy donors was purchased from two sources as follows: seven donors from AllCells, Inc. (Alameda, CA), and 61 donors from HemaCare Corp. (San | Full Paper |
2014 | Edwards, W;Fung-Leung, WP;Huang, C;Chi, E;Wu, N;Liu, Y;Maher, MP;Bonesteel, R;Connor, J;Fellows, R;Garcia, E;Lee, J;Lu, L;Ngo, K;Scott, B;Zhou, H;Swanson, RV;Wickenden, AD; | Targeting the ion channel Kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-life | The Journal of biological chemistry | 24939846 | Human PBMCs were obtained from AllCells LLC or purified from healthy donor blood by Ficoll-Paque (GE Healthcare) density centrifugation. Purified PBMCs were plated in 96-well Save | Full Paper |
2014 | Stuible, M;Moraitis, A;Fortin, A;Saragosa, S;Kalbakji, A;Filion, M;Tremblay, GB; | Mechanism and function of monoclonal antibodies targeting siglec-15 for therapeutic inhibition of osteoclastic bone resorption | The Journal of biological chemistry | 24446437 | HOPs (CD14 + peripheral blood mononuclear cells) were isolated from normal human peripheral blood mononuclear cells (AllCells, Emeryville, CA) using CD14 microbeads and MS Save | Full Paper |
2014 | Collins, E;Gu, F;Qi, M;Molano, I;Ruiz, P;Sun, L;Gilkeson, GS; | Differential efficacy of human mesenchymal stem cells based on source of origin | Journal of immunology (Baltimore, Md. : 1950) | 25274529 | Human BM MSCs were derived from fresh whole BM of healthy donors (purchased from ALLCELLS) and four lupus patients. Lupus patients consisted of four African Americans (three Save | Full Paper |
2014 | Lan, J;Yang, K;Byrd, D;Hu, N;Amet, T;Shepherd, N;Desai, M;Gao, J;Gupta, S;Sun, Y;Yu, Q; | Provirus activation plus CD59 blockage triggers antibody-dependent complement-mediated lysis of latently HIV-1-infected cells | Journal of immunology (Baltimore, Md. : 1950) | 25149467 | Briefly, leukapheresis products from 3 HIV-1-negative blood donors were purchased from the ALLCells (Emeryville, CA) for isolation of primary CD4+ T cells using the negative selection RosetteSep reagents (StemCell Technologies, Vancouver, BC, Canada) | Full Paper |
2014 | Balan, S;Ollion, V;Colletti, N;Chelbi, R;Montanana-Sanchis, F;Liu, H;Vu Manh, TP;Sanchez, C;Savoret, J;Perrot, I;Doffin, AC;Fossum, E;Bechlian, D;Chabannon, C;Bogen, B;Asselin-Paturel, C;Shaw, M;Soos, T;Caux, C;Valladeau-Guilemond, J;Dalod, M; | Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells | Journal of immunology (Baltimore, Md. : 1950) | 25009205 | CD34 + cells from adult donor bone marrow or mobilized peripheral blood were purchased from properly licensed commercial companies (AllCells and StemCells). Save | Full Paper |
2014 | Kajiwara, Y;Schiff, T;Voloudakis, G;Gama Sosa, MA;Elder, G;Bozdagi, O;Buxbaum, JD; | A critical role for human caspase-4 in endotoxin sensitivity | Journal of immunology (Baltimore, Md. : 1950) | 24879791 | Human monocyte-derived macrophages were purchased from AllCells (PB-MDM-001F; Alameda, CA) and cultured in RPMI 1640 supplemented with 10% FBS/penicillin/streptomycin and 10 ng/ml human M-CSF (R&D Systems). BMDM cultures were prepared according to pr | Full Paper |
2014 | Kohn, LA;Seet, CS;Scholes, J;Codrea, F;Chan, R;Zaidi-Merchant, S;Zhu, Y;De Oliveira, S;Kapoor, N;Shah, A;Abdel-Azim, H;Kohn, DB;Crooks, GM; | Human lymphoid development in the absence of common γ-chain receptor signaling | Journal of immunology (Baltimore, Md. : 1950) | 24771849 | Normal human adult BM was obtained from healthy donors as anonymous waste materials isolated from filters after BM harvest and from commercial sources (AllCells, Alameda, CA). Save | Full Paper |
2014 | Inage, E;Kasakura, K;Yashiro, T;Suzuki, R;Baba, Y;Nakano, N;Hara, M;Tanabe, A;Oboki, K;Matsumoto, K;Saito, H;Niyonsaba, F;Ohtsuka, Y;Ogawa, H;Okumura, K;Shimizu, T;Nishiyama, C; | Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2 | Journal of immunology (Baltimore, Md. : 1950) | 24639354 | Human primary mast cells were generated from peripheral blood CD34+ cells (Frozen Mobilized Peripheral Blood CD34+ cells, mPB015F; ALLCELLS, Emeryville, CA) as progenitors based on the previously established method (13). In three independent experime | Full Paper |
2014 | Fukahori, H;Chida, N;Maeda, M;Tasaki, M;Kawashima, T;Matsuoka, H;Suzuki, K;Ishikawa, T;Tanaka, A;Higashi, Y; | Effect of AS2521780, a novel PKCθ selective inhibitor, on T cell-mediated immunity | European journal of pharmacology | 25445039 | T cell-mediated immunity is central to the pathogenesis of autoimmune diseases, and is a target in the development of alternative therapeutic strategies with reduced adverse effects on Save | Full Paper |
2014 | Kota, DJ;DiCarlo, B;Hetz, RA;Smith, P;Cox, CS;Olson, SD; | Differential MSC activation leads to distinct mononuclear leukocyte binding mechanisms | Scientific reports | 24691433 | For Supplemental Figure 1, we also isolated MSCs from commercially available fresh bone marrow aspirates (AllCells, Emeryville, CA) according to the protocols previously established Save | Full Paper |
2014 | Lee, JS;Kim, YR;Park, JM;Ha, SJ;Kim, YE;Baek, NI;Hong, EK; | Mulberry fruit extract protects pancreatic β-cells against hydrogen peroxide-induced apoptosis via antioxidative activity | Molecules (Basel, Switzerland) | 24972274 | and then 0.7 mM hydrogen peroxide was added to allcells for 4 h, with the exception of the h and then 0.7 mM hydrogen peroxide was added to allcells for 4 h, with the exception of the Save | Full Paper |
2014 | Langevin, SM;Houseman, EA;Accomando, WP;Koestler, DC;Christensen, BC;Nelson, HH;Karagas, MR;Marsit, CJ;Wiencke, JK;Kelsey, KT; | Leukocyte-adjusted epigenome-wide association studies of blood from solid tumor patients | Epigenetics | 24671036 | Briefly, sorted non-pathological human peripheral blood leukocytes (n = 47) were previously obtained from different anonymous individuals through AllCells, LLC. Leukocytes were Save | Full Paper |
2014 | Xiao, W;Wang, J;Li, H;Xia, D;Yu, G;Yao, W;Yang, Y;Xiao, H;Lang, B;Ma, X;Guo, X;Guan, W;Xu, H;Liu, J;Zhang, X;Ye, Z; | Fibulin-1 is epigenetically down-regulated and related with bladder cancer recurrence | BMC cancer | 25234557 | O2 maintained at 37°C. SV-HUC-1 was also purchased from ATCC and maintained in DMEM/F-12 medium. Primary human umbilical vein endothelial cell (HUVEC) and Endothelial Cell Medium were purchased from ALLCELLS, LLC (Shanghai, China). Plasmid transfecti | Full Paper |
2014 | Thorarensen, A;Banker, ME;Fensome, A;Telliez, JB;Juba, B;Vincent, F;Czerwinski, RM;Casimiro-Garcia, A; | ATP-mediated kinome selectivity: the missing link in understanding the contribution of individual JAK Kinase isoforms to cellular signaling | ACS chemical biology | 24814050 | Cryopreserved human PBMCs (no. PB005F), which were used in the IL-2, IL-10, IL-15, and IFNα assays, were purchased from Allcells (Emeryville, CA). Frozen PBMCs were thawed in a water bath (37 °C) and washed once with once with 5% FBS/RPMI-1640 | Full Paper |
2014 | Womble, TA;Green, S;Shahaduzzaman, M;Grieco, J;Sanberg, PR;Pennypacker, KR;Willing, AE; | Monocytes are essential for the neuroprotective effect of human cord blood cells following middle cerebral artery occlusion in rat | Molecular and cellular neurosciences | 24472845 | The cells (either HUCB or HPB MNC, obtained commercially from AllCells, LLC), were thawed rapidly at 37 C and transferred slowly into 10 ml of 10 mM PBS. The cells were then Save | Full Paper |
2014 | Fraser, P;Macdonald, G;Mullineux, A; | Regional Monetary Policy: An Australian Perspective | Regional Studies | This is a feature which has been under discussion and analysis for some time (for example, Tremosa-Ballcells and Pons-Novell, 2001; Mojon and Peersman, 2001; Aksoy et al., 2002; Clausen and Wohltmann, 2005; and more recently, Dow et al., 2012; and al | Full Paper | |
2014 | Liu, X;Ou, YC;Zhang, J;Ahene, A;Clark, D;Hsieh, SC;Cooper, M;Ji, C; | Pharmacokinetics and pharmacodynamics of CD4-anchoring bi-functional fusion inhibitor in monkeys | Pharmaceutical research | 24065594 | BD Biosciences (La Jolla, CA). Human PBMCs from healthy donors were purchased from AllCells (cat # PB001, Emeryville, CA) and cultured in complete medium (RPMI 1640, 10% FBS, 1% P/S, 1% L-Glutamine, 1% non-essential amino acid, 1% sodium pyruvate, 1% | Full Paper |
2014 | Wolstein, O;Boyd, M;Millington, M;Impey, H;Boyer, J;Howe, A;Delebecque, F;Cornetta, K;Rothe, M;Baum, C;Nicolson, T;Koldej, R;Zhang, J;Keech, N;Camba Colón, J;Breton, L;Bartlett, J;An, DS;Chen, IS;Burke, B;Symonds, GP; | Preclinical safety and efficacy of an anti-HIV-1 lentiviral vector containing a short hairpin RNA to CCR5 and the C46 fusion inhibitor | Molecular therapy. Methods & clinical development | 26015947 | ced overnight on RetroNectin (TaKaRa, Japan, #T100B) coated plates at various MOI, as indicated in the experimental methodology. CD34+ cells Primary human CD34+ HSPC were purchased commercially from AllCells; cells were directly isolated from granul | Full Paper |
2014 | Palasuk, J;Kamocki, K;Hippenmeyer, L;Platt, JA;Spolnik, KJ;Gregory, RL;Bottino, MC; | Bimix antimicrobial scaffolds for regenerative endodontics | Journal of endodontics | 25201643 | Introduction Eliminating and/or inhibiting bacterial growth within the root canal system has been shown to play a key role in the regenerative outcome. The aim of this study was to Save | Full Paper |
2014 | Zhang, HT;Wang, L;Ai, J;Chen, Y;He, CX;Ji, YC;Huang, M;Yang, JY;Zhang, A;Ding, J;Geng, MY; | SOMG-833, a novel selective c-MET inhibitor, blocks c-MET-dependent neoplastic effects and exerts antitumor activity | The Journal of pharmacology and experimental therapeutics | 24741075 | HUVEC cells (passage 3) were first serum starved in complete medium (AllCells) for 24 hours and treated by SOMG-833 for 72 hours in media containing 3% bovine serum albumin and Save | Full Paper |
2014 | Kimbrel, EA;Kouris, NA;Yavanian, GJ;Chu, J;Qin, Y;Chan, A;Singh, RP;McCurdy, D;Gordon, L;Levinson, RD;Lanza, R; | Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic properties | Stem cells and development | 24650034 | from fresh BM aspirates (AllCells LLC, Alameda, CA) using Histopaque 1077/Accuspin tubes (Sigma-Aldrich) or from frozen human BM mononuclear cells (AllCells LLC) from both male Save | Full Paper |
2014 | Iwata, M;Sandstrom, RS;Delrow, JJ;Stamatoyannopoulos, JA;Torok-Storb, B; | Functionally and phenotypically distinct subpopulations of marrow stromal cells are fibroblast in origin and induce different fates in peripheral blood monocytes | Stem cells and development | 24131213 | The cells were cultured in mesenchymal stromal cell (MSC) basal media with MSC stimulatory supplement according to the manufacturer's protocol (ALLCELLS), and then expanded in Save | Full Paper |
2014 | Johnston, JM;Denning, G;Moot, R;Whitehead, D;Shields, J;Le Doux, JM;Doering, CB;Spencer, HT; | High-throughput screening identifies compounds that enhance lentiviral transduction | Gene therapy | 25231175 | Fresh CD34+ cells were purchased from AllCells (Emeryville, CA, USA). Before arrival, CD34+ cells were mobilized from human bone marrow and isolated from the peripheral blood of three individual subjects. Flow cytometry was used to assess the purity | Full Paper |
2014 | Kamata, S;Miyagawa, S;Fukushima, S;Nakatani, S;Kawamoto, A;Saito, A;Harada, A;Shimizu, T;Daimon, T;Okano, T;Asahara, T;Sawa, Y; | Improvement of cardiac stem cell sheet therapy for chronic ischemic injury by adding endothelial progenitor cell transplantation: analysis of layer-specific regional cardiac function | Cell transplantation | 23562134 | Each CSC sheet was approximately 42 mm in diameter and 100 μm thick. Granulocyte colony-stimulating factor-mobilized EPCs of human origin (AllCells, MPB-017F; Emeryville, CA, USA) were labeled with 2 μM DiI-blue in vitro (Molecular Probes) (33). The | Full Paper |
2014 | Xiao, H;Li, H;Yu, G;Xiao, W;Hu, J;Tang, K;Zeng, J;He, W;Zeng, G;Ye, Z;Xu, H; | MicroRNA-10b promotes migration and invasion through KLF4 and HOXD10 in human bladder cancer | Oncology reports | 24573354 | SV-HUC-1 was also purchased from ATCC and maintained in DMEM/F-12 medium. SCaBER was purchased from AllCells, LLC (Shanghai, China) in MEM medium. Paired samples of primary BC, and lymph node metastatic tissues were obtained from patients who had und | Full Paper |
2014 | Czubak, P;Bojarska-Junak, A;Tabarkiewicz, J;Putowski, L; | A modified method of insulin producing cells' generation from bone marrow-derived mesenchymal stem cells | Journal of diabetes research | 25405207 | Human bone marrow stromal cells (MSCs) were purchased from a commercial source (AllCells; catalog number MSC-001F). This cell line was derived from one, nondiabetic Caucasian Save | Full Paper |
2014 | Gupta, MS;Nicoll, SB; | Functional nucleus pulposus-like matrix assembly by human mesenchymal stromal cells is directed by macromer concentration in photocrosslinked carboxymethylcellulose hydrogels | Cell and tissue research | 25092545 | Human MSCs were purified from bone marrow (AllCells, Emeryville, CA, USA) using enriched density centrifugation as previously described (Gupta et al. 2011; Chen et al. 2013). Briefly, a Human MSC Enrichment Cocktail (StemCell Technologies, Vancouver, | Full Paper |
2014 | Pavlides, S;Gutierrez-Pajares, JL;Iturrieta, J;Lisanti, MP;Frank, PG; | Endothelial caveolin-1 plays a major role in the development of atherosclerosis | Cell and tissue research | 24390341 | Human umbilical vein endothelial cells (HUVEC) were obtained from AllCells (AllCells, LLC, Emeryville, CA, USA). HUVEC were cultured in M199 media (Life Technologies, Grand Island, NY, USA) containing 10 % FBS (Life Technologies, Grand Island, NY, US | Full Paper |
2014 | Kiang, JG;Garrison, BR;Smith, JT;Fukumoto, R; | Ciprofloxacin as a potential radio-sensitizer to tumor cells and a radio-protectant for normal cells: differential effects on γ-H2AX formation, p53 phosphorylation, Bcl-2 production, and cell death | Molecular and cellular biochemistry | 24802382 | Human B lymphoblastoid cell line TK6 (p53+/+) and human NH32 (p53−/− of TK6 cells) were generous gifts from Dr. James Mitchell. Human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Emeryville, CA). Cells were grown in RPMI 1 | Full Paper |
2014 | Dong, B;Zhou, H;Han, C;Yao, J;Xu, L;Zhang, M;Fu, Y;Xia, Q; | Ischemia/reperfusion-induced CHOP expression promotes apoptosis and impairs renal function recovery: the role of acidosis and GPR4 | PloS one | 25343248 | Human umbilical vein endothelial cells (HUVECs), as well as supplier's recommended medium and supplement were purchased from ALLCELLS (Emeryville, California, USA). Human Save | Full Paper |
2014 | Iwata, M;Torok-Storb, B;Wayner, EA;Carter, WG; | CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine production | PloS one | 25275584 | The cells were cultured in MSC Basal media with MSC stimulatory supplement according to the manufacturer's protocol (ALLCELLS, Emeryville, CA). The cells were passaged until the Save | Full Paper |
2014 | Ayala-Lujan, JL;Vijayakumar, V;Gong, M;Smith, R;Santiago, AE;Ruiz-Perez, F; | Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes | PloS one | 25251283 | Fresh human blood to isolate primary cells were purchased from Allcells LLC. (Alameda, Fresh heparinized blood from healthy human donors purchased from AllCells LLC. (Alameda, Save | Full Paper |
2014 | Kang, Y;Hodges, A;Ong, E;Roberts, W;Piermarocchi, C;Paternostro, G; | Identification of drug combinations containing imatinib for treatment of BCR-ABL+ leukemias | PloS one | 25029499 | , USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, USA), 100 U/ml penicillin, 100 µg/ml streptomycin (Hyclone, USA) at 37°C in 5% CO2. CD34+ hematopoietic stem cells were purchased from Allcells and maintained in StemSpan SFEM (STEMCELL T | Full Paper |
2014 | Lewis, ND;Patnaude, LA;Pelletier, J;Souza, DJ;Lukas, SM;King, FJ;Hill, JD;Stefanopoulos, DE;Ryan, K;Desai, S;Skow, D;Kauschke, SG;Broermann, A;Kuzmich, D;Harcken, C;Hickey, ER;Modis, LK; | A GPBAR1 (TGR5) small molecule agonist shows specific inhibitory effects on myeloid cell activation in vitro and reduces experimental autoimmune encephalitis (EAE) in vivo | PloS one | 24967665 | Human peripheral blood mononuclear cells isolated with leukapheresis were purchased from AllCells (Alameda, CA), frozen in 80% FBS, 10% RPMI and 10% DMSO and stored in Save | Full Paper |
2014 | Simeonov, KP;Uppal, H; | Direct reprogramming of human fibroblasts to hepatocyte-like cells by synthetic modified mRNAs | PloS one | 24963715 | ield of direct reprogramming can overcome its retroviral reliance and be translated to its ultimate applications in regenerative medicine. Materials and Methods Cell Culture CD34+ bone marrow cells (AllCells, Emeryville, CA) were received fresh and | Full Paper |
2014 | Hong, KU;Guo, Y;Li, QH;Cao, P;Al-Maqtari, T;Vajravelu, BN;Du, J;Book, MJ;Zhu, X;Nong, Y;Bhatnagar, A;Bolli, R; | c-kit+ Cardiac stem cells alleviate post-myocardial infarction left ventricular dysfunction despite poor engraftment and negligible retention in the recipient heart | PloS one | 24806457 | m (A LV) which included the infarcted and border zones, posterior LV wall (nonischemic region; P LV), and atria+right ventricle. Female human peripheral mononuclear cells (hPBMCs) were purchased from AllCells, LLC (Emeryville, CA). Aliquots of 105 hP | Full Paper |
2014 | Gañán-Gómez, I;Wei, Y;Yang, H;Pierce, S;Bueso-Ramos, C;Calin, G;Boyano-Adánez, Mdel C;García-Manero, G; | Overexpression of miR-125a in myelodysplastic syndrome CD34+ cells modulates NF-κB activation and enhances erythroid differentiation arrest | PloS one | 24690917 | Human bone marrow cells from 6 healthy individuals were obtained from AllCells (Emeryville, CA). Isolation of CD34 + cells was performed using MicroBead Kit (Miltenyi, Bergisch Save | Full Paper |
2014 | Chyu, KY;Lio, WM;Dimayuga, PC;Zhou, J;Zhao, X;Yano, J;Trinidad, P;Honjo, T;Cercek, B;Shah, PK; | Cholesterol lowering modulates T cell function in vivo and in vitro | PloS one | 24647529 | ential batch-to-batch differences in the biochemical contents in the FBS which may affect the experimental outcomes. The same experiment was performed using human peripheral blood mononuclear cells (AllCells, Emeryville, CA). T cells were isolated f | Full Paper |
2014 | Chen, J;Li, C;Wang, S; | Periodic heat shock accelerated the chondrogenic differentiation of human mesenchymal stem cells in pellet culture | PloS one | 24632670 | nd Methods All reagents and chemicals without manufacture labels were purchased from Sigma-Aldrich (St Louis, MO). hMSC Isolation and Characterization Human MSCs were isolated from bone marrow (BM) (AllCells LLC, Berkeley, CA) as described previousl | Full Paper |
2014 | Wang, J;Guo, X;Lui, M;Chu, PJ;Yoo, J;Chang, M;Yen, Y; | Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro | PloS one | 24465489 | This study was conducted using data obtained from 70 fresh hPB and 30 fresh hBM samples purchased from AllCells, LLC. A list of the reagents and antibodies used in this study is Save | Full Paper |
2014 | Beckwith, R;Worden, E;Estrin, E;Martin, A; | Reconstitution of the 26S Proteasome Reveals Functional Asymmetries in its Heterohexameric AAA+ Unfoldase | Biophysical Journal | Proteins are complex and versatile macromolecules that are integral components of allcells. Among other things, proteins are responsible for structural organization, signalingand Save | Full Paper | |
2014 | Aikawa, N;Kunisato, A;Nagao, K;Kusaka, H;Takaba, K;Ohgami, K; | Detection of thalidomide embryotoxicity by in vitro embryotoxicity testing based on human iPS cells | Journal of pharmacological sciences | 24451995 | The hiPSCs were derived from human bone marrow mononuclear cells which were purchased from AllCells (Emeryville, CA, USA) (11). Human dermal fibroblasts isolated from adult Save | Full Paper |
2014 | Chen, JK;Li, ZP;Liu, YZ;Zhao, T;Zhao, XB;Ni, M;Jiang, GJ;Shen, FM; | Activation of alpha 7 nicotinic acetylcholine receptor protects mice from radiation-induced intestinal injury and mortality | Radiation research | 24828112 | Radiation-induced gastrointestinal syndrome occurs when the body is exposed to a high dose of radiation. Currently, safe and effective radioprotectants are not available. Apoptosis Save | Full Paper |
2014 | Lee, M;Jeong, SY;Ha, J;Kim, M;Jin, HJ;Kwon, SJ;Chang, JW;Choi, SJ;Oh, W;Yang, YS;Kim, JS;Jeon, HB; | Low immunogenicity of allogeneic human umbilical cord blood-derived mesenchymal stem cells in vitro and in vivo | Biochemical and biophysical research communications | 24657442 | Abstract Evaluation of the immunogenicity of human mesenchymal stem cells (MSCs) in an allogeneic setting during therapy has been hampered by lack of suitable models due to Save | Full Paper |
2014 | Shima, T;Miyamoto, T;Kikushige, Y;Yuda, J;Tochigi, T;Yoshimoto, G;Kato, K;Takenaka, K;Iwasaki, H;Mizuno, S;Goto, N;Akashi, K; | The ordered acquisition of Class II and Class I mutations directs formation of human t(8;21) acute myelogenous leukemia stem cell | Experimental hematology | 25101977 | Human marrow was purchased from AllCells (Emeryville, CA). Informed consent was obtained from all patients. The Institutional Review Board of Kyushu University Hospital (Fukuoka, Save | Full Paper |
2014 | Iancu-Rubin, C;Mosoyan, G;Glenn, K;Gordon, RE;Nichols, GL;Hoffman, R; | Activation of p53 by the MDM2 inhibitor RG7112 impairs thrombopoiesis | Experimental hematology | 24309210 | Human MK were generated in a two-step liquid culture system from bone marrow (BM)-derived CD34+ cells (AllCells, Emeryville, CA, USA). Cells were suspended in IMDM-based MK differentiation medium [6] for 7 days then allowed to mature for 7-9 addition | Full Paper |
2014 | Kornblau, SM;Cohen, AC;Soper, D;Huang, YW;Cesano, A; | Age-related changes of healthy bone marrow cell signaling in response to growth factors provide insight into low risk MDS | Cytometry. Part B, Clinical cytometry | 24106013 | patients aged 54 to 82 years undergoing planned orthopedic procedures at Williamson Medical Center in Tennessee. The second (“younger”) healthy sample set consisted of 6 cryopreserved BMMC obtained from donors aged 23 to 43 years, purchased from a co | Full Paper |
2014 | Barrionuevo, M; | Society of Toxicology (SOT) 5st Annual Meeting and ToxExpo, San Francisco, CA, USA, March 11-15, 2012 | Drugs of the Future | 283 on human myeloid, erythroid and mesenchymal lineages for its potential to protect marrow progenitor cells from radiation damage was discussed by Emer Clarke from ReachBio. Save | Full Paper | |
2014 | Fu, Y;Chen, YL;Herve, M;Gu, F;Shi, PY;Blasco, F; | Development of a FACS-based assay for evaluating antiviral potency of compound in dengue infected peripheral blood mononuclear cells | Journal of virological methods | 24140514 | Isolated cryopreserved human PBMCs were obtained from AllCells, LLC. The PBMCs were obtained with informed consent and experiments involving PBMCs were approved by Save | Full Paper |
2014 | Tilton, CA;Tabler, CO;Lucera, MB;Marek, SL;Haqqani, AA;Tilton, JC; | A combination HIV reporter virus system for measuring post-entry event efficiency and viral outcome in primary CD4+ T cell subsets | Journal of virological methods | 24025341 | Peripheral blood mononuclear cells (PBMCs) and CD4+ T cells were obtained from leukopheresis from ALLCELLS, LLC on five healthy donors. All subjects for this study were de- Save | Full Paper |
2014 | Shiheido, H;Kitagori, K;Sasaki, C;Kobayashi, S;Aoyama, T;Urata, K;Oku, T;Hirayama, Y;Yoshitomi, H;Hikida, M;Yoshifuji, H;Mimori, T;Watanabe, T;Shimizu, J; | Human T cells expressing BEND3 on their surface represent a novel subpopulation that preferentially produces IL-6 and IL-8 | Immunity, inflammation and disease | 25400923 | , #16-15 Abs, and 7AAD, and BEND3+ or BEND3− subpopulations in CD4+ or CD8+ T cells were sorted using a flow cytometer (FACSAria, BD Biosciences, San Jose, CA). Cord blood cells were purchased from ALLCELLS, and Jurkat [25] cells were from the Americ | Full Paper |
2014 | Cheng, ZJ;Garvin, D;Paguio, A;Moravec, R;Engel, L;Fan, F;Surowy, T; | Development of a robust reporter-based ADCC assay with frozen, thaw-and-use cells to measure Fc effector function of therapeutic antibodies | Journal of immunological methods | 25086226 | PBMCs from different donors were from AllCells. PNGase F, Magne™ Protein G Beads, Bio-Glo™ Luciferase Assay System and CytoTox 96® Non-Radioactive Cytotoxicity Assay were from Promega | Full Paper |
2014 | Furuya, T;Sá, JM;Chitnis, CE;Wellems, TE;Stedman, TT; | Reticulocytes from cryopreserved erythroblasts support Plasmodium vivax infection in vitro | Parasitology international | 24291603 | Plasmodium vivax is the most widely distributed human malaria parasite. Despite its importance, both clinical research and basic research have been hampered by lack of a convenient Save | Full Paper |
2014 | Meissner, TB;Mandal, PK;Ferreira, LM;Rossi, DJ;Cowan, CA; | Genome editing for human gene therapy | Methods in enzymology | 25398345 | CD34 + cells from G-CSF mobilized peripheral blood were purchased from AllCells. CD34 + cells were cotransfected with CRISPR/Cas9 expression plasmids using Amaxa Save | Full Paper |
2014 | Wilson, EM;Bial, J;Tarlow, B;Bial, G;Jensen, B;Greiner, DL;Brehm, MA;Grompe, M; | Extensive double humanization of both liver and hematopoiesis in FRGN mice | Stem cell research | 25310256 | rich Chemicals; non-platable human hepatocytes (> 70% viable by trypan blue staining) were purchased from BioreclamationIVT (catalog# F00995); human frozen bone marrow CD34+ cells were purchased from AllCells (Catalog# ABM017F); HCM media was purchas | Full Paper |
2014 | Yourek, G;Xin, X;Reilly, GC;Mao, JJ; | Infiltration of mesenchymal stem cells into PEGDA hydrogel | Bio-medical materials and engineering | 25201394 | Human MSCs were isolated from commercially available whole bone marrow (AllCells, Berkeley, CA) using "RosetteSep " (StemCell Technologies; Vancouver, BC) according to the Save | Full Paper |
2014 | Chang, TP;Vancurova, I; | Bcl3 regulates pro-survival and pro-inflammatory gene expression in cutaneous T-cell lymphoma | Biochimica et biophysica acta | 25089799 | The advanced stages of cutaneous T cell lymphoma (CTCL) are characterized not only by decreased levels of pro-inflammatory cytokines, resulting in high susceptibility to infections, Save | Full Paper |
2014 | Li, J;Sun, W;Subrahmanyam, PB;Page, C;Younger, KM;Tiper, IV;Frieman, M;Kimball, AS;Webb, TJ; | NKT Cell Responses to B Cell Lymphoma | Medical sciences (Basel, Switzerland) | 24955247 | tained from commercial vendors. Specifically, buffy coats were purchased from Biological Specialty Corporation and peripheral blood from two different, newly diagnosed MCL patients was purchased from AllCells, LLC (Alameda, CA, USA). PBMCs were isola | Full Paper |
2014 | Gitschier, HJ;Bergeron, AB;Randle, DH; | Label-free cell-based dynamic mass redistribution assays | Current protocols in chemical biology | 24652622 | Human umbilical vein endothelial cells (Corning, cat. no. 354151) Peripheral blood mononuclear cells (AllCells, cat. no. PB006F) Appropriate cell culture/differentiation medium with supplements, e.g.: IMDM, KnockOut DMEM/F-12 with Stempro supplement, | Full Paper |
2014 | Vij, R;Mazumder, A;Klinger, M;O'Dea, D;Paasch, J;Martin, T;Weng, L;Park, J;Fiala, M;Faham, M;Wolf, J; | Deep sequencing reveals myeloma cells in peripheral blood in majority of multiple myeloma patients | Clinical lymphoma, myeloma & leukemia | 24629890 | The remaining 13 paired bone marrow and peripheral blood samples were purchased from a commercial source (AllCells, Emeryville, CA). Save | Full Paper |
2014 | DeClerck, Y; | Environment-Mediated Drug Resistance in Neuroblastoma | U.S. Army Medical Research Material Command | Human BMMSC were purchased from AllCells LLC. Monocytes of normal healthy donors were obtained from peripheral blood and separated by Ficoll density gradient centrifugation Save | Full Paper | |
2014 | Kornblau, S; | The Use of “Omics” to Guide the Selection of Targeted Therapy | Targeted Therapy of Acute Myeloid Leukemia | If the normal HSC frequency is 1:100,000, then multiply this by 10. For our protein work, we have therefore used a CD34+ cell from a normal bone marrow as our standard comparator (we have successfully used CD34+ cells from AllCells for our RPPA work) | Full Paper | |
2014 | 허운학, ; | Concurrent treatment with capecitabine and radiosurgery in the mouse glioblastoma model | Thesis | AllCells were cultured for two to three passages in Dulbecco's modified Eagle's medium (WelGENE, Daegu, Korea) containing 10% fetal bovine serum and 1% antibiotic-antimycotic Save | Full Paper | |
2014 | Zaidi-Merchant, SS; | The role of let-7c/miR-125b/miR-99a in the hematopoietic development in the human bone marrow | Thesis | Normal human bone marrow samples were obtained from healthy donors via the UCLA Pathology Tissue Core, Cincinnati Children’s Hospital, or ALLCELLS according to guidelines approved by UCLA Institutional Review Board. Fresh BM samples were Ficoll-Paque | Full Paper | |
2014 | Holm, HH; | A CUDA Back-End for the Equelle Compiler. | Thesis | The function Gradient takes a Collection Of Scalar On AllCells() as input and compute the discrete If u is a variable defined as Collection Of Scalar On AllCells() then the following line is Save | Full Paper | |
2014 | Oien, N; | Erythrocyte Carriers: Tool Development for Erythrocyte Mediated Diagnostic and Drug Delivery | Thesis | Peripheral erythrocytes were purchased from AllCells. Dialysis cassettes were purchased Peripheral red blood cells were purchased from AllCells. 8-(4-chlorophenylthio) adenosine-3′ Save | Full Paper | |
2014 | Nguyen, L; | LONG WAVELENGTH FLUORESCENT REPORTERS FOR PROTEIN KINASES AND DESIGN OF LIGHT-ACTIVATABLE BIOAGENTS | Thesis | H89 and KT5720 were purchased from Sigma-Aldrich and Calbiochem (EMD), respectively. Peripheral red blood cells were purchased from AllCells. 8-(4-chlorophenylthio) adenosine-3′,5′-cyclic monophosphorothioate (CPT-cAMP), ATP, and cAMP were purchased | Full Paper | |
2014 | Rathbone, E; | Inhibitory and Immunomodulatory Properties of Human Choroid Plexus Epithelial Cells | Thesis | Pooled BMSC, ADSC (both Lonza, Basel, Switzerland), DPSC (AllCells L.L.C., Alameda, CA, USA) and AMSC were cultured in high glucose Dulbecco’s Modified Eagle Medium (HG-DMEM; Biosera, Boussens, France) supplemented with 10% foetal bovine serum (FBS), | Full Paper | |
2014 | Suganyaa, MV;Deepa, MKRS;Vinothini, MG; | Operating the SRAM Cell Architecture with the High Efficient and Reduced Memory Size | ijaist.com | It has a multiplicative effect; allcells in the array are drawing this power. The CMOS 8T cellrequires about 60 times more static power than SRAM cells inthis study. The SRAM 8T cell Save | Full Paper | |
2014 | Yu, Y; | Energy prediction of electric floor radiation systems using a new integrated modeling approach | ASHRAE Transactions | In this figure, PMV Onezone and PPD Onezone represent the PMV and PPD values when the one-zone (multi-zone) model is applied and PMV Allcells and PPD Allcells represent the Save | Full Paper | |
2014 | Xiao, F; | Genomic study of familial breast cancer-genomic instability, classification and predisposition | Thesis | The cells for the study were purchased from AllCells LLC (http://www.allcells.com/, Emeryville, California), which supplies human blood cells from donors for research and the company has its full IRB system (Biomed IRB). The signed written consent fo | Full Paper | |
2014 | Perignon, MC; | A Rolling Stone Gathers No Moss: Modeling the Geomorphic Impacts of Floods in the Southern Rocky Mountain Region, USA | Thesis | (Top, right) Non-local survival function for sediment entering the system at the mouth of the fan, for allcells with ow depth greater than 1 cm. (Bottom, right) Non-local probability of Save | Full Paper | |
2014 | Suwannatat, P; | A Visual Analysis Toolkit for Microscopic Image Mosaics of Retinal Astrocytes | Thesis | 5 The cell locations are kept at allcells.txt under the data directory. as a gray cell among multi-hue neighbors with 100% saturations. with a "new cell here" command of the mouse Save | Full Paper | |
2014 | Johnson, SM; | Extracellular matrix improves mesenchymal stem cell growth and maintenance of stemness in serum free medium | Thesis | Freshly isolated human bone marrow mononuclear cells, containing BM-MSCs (primary cells) from 20-25 year old donors were purchased from ALLCELLS (Emeryville, CA). Greater Save | Full Paper | |
2014 | Tsai, HC; | Molecular Mechanism in the Regulation of Human Th17 Cell Differentiation and Function | Thesis | The use of human cells in the study was periodically reviewed and approved by the Human Subject Research Review Committee (University of California, Davis). PBMCs were purchased from Allcells. LLC. Samples used in a single experiment were from differ | Full Paper | |
2014 | Spinler, KR; | Membrane forces and key protein determinants of hematopoietic cell function: Lamins and myosin-II in hematopoiesis and CD47 in immunotherapy of cancer | Thesis | Fresh purified bone marrow-derived human CD34+ cells and total mononuclear cells were obtained from either the Penn Xenograft Core Facility or AllCells (Emeryville, CA). Cells from at least five different donors were used in this study. Purity of th | Full Paper | |
2014 | Amir, ED; | viSNE and Wanderlust, two algorithms for the visualization and analysis of high-dimensional single-cell data | Thesis | Fresh, Ficoll-enriched human bone marrow was obtained from healthy donors from AllCells, Inc. (Emeryville, CA). Samples were obtained with informed consent in accordance with the Declaration of Helsinki and with accordance with Stanford University’s | Full Paper | |
2014 | Qin, S; | The use of biomaterials for cell function enhancement: acceleration of fibroblast migration and promotion of stem cell proliferation | Thesis | Human cord blood CD34+ cells were purchased from AllCells LLC and cultured in StemSpan SFEM media, 10% FBS, 100 ng/ml each of human SCF, TPO, and Flt-3L. Cells were infected with ICD-Notch or GFP control lentiviral particles then plated on hydrogels | Full Paper | |
2014 | Krzeszinski, JY;Wei, W;Huynh, H;Jin, Z;Wang, X;Chang, TC;Xie, XJ;He, L;Mangala, LS;Lopez-Berestein, G;Sood, AK;Mendell, JT;Wan, Y; | miR-34a blocks osteoporosis and bone metastasis by inhibiting osteoclastogenesis and Tgif2 | Nature | 25043055 | Human PBMN cells (ReachBio) were differentiated into osteoclasts in α-MEM containing 10% FBS, 25 ng m1 −l MCSF, 50 ng m1 −l hRANKL, 1 μM Dexamethasome and 1 μM Save;odeoxyuridine (BrdU) cell proliferation assay kit (GE Healthcare) as described27. O | Full Paper |
2014 | Shah, N;Cortes, J;Martinelli, G;Smith, B;Clarke, E;Copland, M;Strauss, L;Talpaz, M; | Dasatinib Plus Smoothened (SMO) Inhibitor BMS-833923 in Chronic Myeloid Leukemia (CML) with Resistance or Suboptimal Response to a Prior Tyrosine Kinase Inhibitor (TKI): Phase I Study CA180323 | Blood | University of California, San Francisco, San Francisco, CA++The University of Texas MD Anderson Cancer Center, Houston, TX++University of Bologna, Bologna, Italy++Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD++ReachBio LLC | Full Paper | |
2014 | Chattopadhyay, R;Dyukova, E;Singh, NK;Ohba, M;Mobley, JA;Rao, GN; | Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C ε-mediated zona occludens-1 phosphorylation at threonine 770/772 | The Journal of biological chemistry | 24338688 | The human peripheral blood CD14+ monocytes were purchased from ReachBio LLC (Seattle, WA), suspended in RPMI 1640 medium containing 50 μm 2-mercaptoethanol, 100 IU/ml penicillin, and 100 μg/ml streptomycin, and used for transmigration assay. To colle | Full Paper |
2014 | Winter, JM;Jacobson, P;Bullough, B;Christensen, AP;Boyer, M;Reems, JA; | Long-term effects of cryopreservation on clinically prepared hematopoietic progenitor cell products | Cytotherapy | 24910385 | CFC assays were performed 21, 22 with either commercially available MethoCult (StemCell Technologies Inc, Vancouver, Canada) or ColonyGel (ReachBio, LLC, Seattle, WA, USA) that had been internally validated to show comparable results. Briefly, HPC(A) | Full Paper |
2014 | Chandrasekaran, D;Nakamoto, B;Watts, KL;Kiem, HP;Papayannopoulou, T; | Modeling promising nonmyeloablative conditioning regimens in nonhuman primates | Human gene therapy | 24937231 | Transduced, mock-transduced, and BM white blood cell samples from transplanted macaques were cultured in semisolid methylcellulose medium for 12-14 days in the presence of rhIL-3, recombinant human erythropoietin (rhEPO), rhSCF, and recombinant human | Full Paper |
2014 | Cook, GJ;Caudell, DL;Elford, HL;Pardee, TS; | The efficacy of the ribonucleotide reductase inhibitor Didox in preclinical models of AML | PloS one | 25402485 | bated as indicated above for 24 hours with a titration of Didox. Cells were washed with PBS and resuspended in IMDM supplemented with 20% FBS, and placed in ColonyGel High Cytokine Formulation media (ReachBio). Experiments were performed in triplicat | Full Paper |
2014 | Byon, JC;Padilla, SM;Papayannopoulou, T; | Deletion of Dicer in late erythroid cells results in impaired stress erythropoiesis in mice | Experimental hematology | 24971698 | Colony Forming Assay Peripheral blood, spleen and bone marrow mononuclear cells were plated in methylcellulose containing hematopoietic growth factors (ReachBio, WA) as previously described [17] | Full Paper |
2014 | Li, X;Xu, X;Ji, T;Liu, Z;Gu, M;Hoang, BH;Zi, X; | Dietary feeding of Flavokawain A, a Kava chalcone, exhibits a satisfactory safety profile and its association with enhancement of phase II enzymes in mice | Toxicology reports | 25379458 | ated, the yield and viability of cells were determined by Trypan blue exclusion method. A total number of 2 × 104 cells were seeded in a 6-well plate containing Colony Gel 1201 Mouse Base Medium (Reachbio), and then treated with FKA and Adriamycin a | Full Paper |
2014 | Erythroid, IVEOS; | Progenitor Populations And Analogous Human Stress Erythroid Progenitors | Thesis | Human bone marrow mononuclear cells were purchased from Reachbio [26-‐200] and cultured in the stress erythropoiesis medium described above. On the indicated days, cells were Save | Full Paper | |
2014 | Smuda, CA; | The Transcriptional Regulator Ikaros and Control of Mast Cell and Basophil Development | Thesis | We took advantage of the sorting strategies developed for quantitation of progenitor subtypes to isolate these populations for further analysis in solid culture media. IL-3 (5 ng/mL) and SCF (12.5 ng/mL) was added ColonyGel 1201 base methylcellulose | Full Paper | |
2013 | Singh, R;Low, ET;Ooi, LC;Ong-Abdullah, M;Ting, NC;Nagappan, J;Nookiah, R;Amiruddin, MD;Rosli, R;Manaf, MA;Chan, KL;Halim, MA;Azizi, N;Lakey, N;Smith, SW;Budiman, MA;Hogan, M;Bacher, B;Van Brunt, A;Wang, C;Ordway, JM;Sambanthamurthi, R;Martienssen, RA; | The oil palm SHELL gene controls oil yield and encodes a homologue of SEEDSTICK | Nature | 23883930 | We thank Johannes Jansen (PRI, Wageningen, Netherlands) for assistance with JoinMap, and Beijing Genome Institute, The Genome Institute at Washington University, Tufts University Core Facility and the Arizona Genome Institute for mapping and genome s | Full Paper |
2013 | Ong, CT;Van Bortle, K;Ramos, E;Corces, VG; | Poly(ADP-ribosyl)ation regulates insulator function and intrachromosomal interactions in Drosophila | Cell | 24055367 | We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology for their help in performing Illumina sequencing of ChIP-Seq samples. This work was supported by U.S. Public Health Service Award R01 GM035463 from the Nat | Full Paper |
2013 | Mukhopadhyay, S;Wen, X;Ratti, N;Loktev, A;Rangell, L;Scales, SJ;Jackson, PK; | The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling | Cell | 23332756 | For the ISH experiments using radiolabeled probes, custom service from Phylogeny Inc. (Columbus, OH) was used. Both antisense and sense radiolabeled riboprobes were synthesized in vitro according to the manufacturer’s specifications (Ambion) and labe | Full Paper |
2013 | Locke, D;Bernstein, S;Lynch, F;Siami-Namini, K;Walling, J;Yonker, T;Yarranton, G; | An IHC Screen For EphA3 Positive FFPE Tumors | Blood | November 15, 2013. An IHC Screen For EphA3 Positive FFPE Tumors. Darren Locke, PhD Darren Locke, PhD. 1. QualTek Molecular Laboratories, Newtown, USA,. 1. Search for other works by this author on: This Site. PubMed. Google | Full Paper | |
2013 | Yang, J;Sung, E;Donlin-Asp, PG;Corces, VG; | A subset of Drosophila Myc sites remain associated with mitotic chromosomes colocalized with insulator proteins | Nat Commun | 23403565 | ChIP was performed with ~4 × 107 cells. Cells were cross-linked with 1% formaldehyde for 10 min at room temperature. Nuclear lysates were sonicated to generate 200– 1000 bp DNA fragments. ChIP was then performed with 6 μL of Drosophila α-Myc antibody | Full Paper |
2013 | Day, K;Waite, LL;Thalacker-Mercer, A;West, A;Bamman, MM;Brooks, JD;Myers, RM;Absher, D; | Differential DNA methylation with age displays both common and dynamic features across human tissues that are influenced by CpG landscape | Genome Biol. | 24034465 | ;... samples All tissue samples were collected according to protocols and guidelines at their respective institutions. Iron deficient anemia buffy coat samples were commercially available and collected by Conversant Bio (Huntsville, AL, USA). Blood D | Full Paper |
2013 | Kellner, WA;Van Bortle, K;Li, L;Ramos, E;Takenaka, N;Corces, VG; | Distinct isoforms of the Drosophila Brd4 homologue are present at enhancers, promoters and insulator sites | Nucleic Acids Res. | 23945939 | Illumina adaptors (Illumina Cat# PE-102–1001) were titrated according to prepared DNA ChIP sample concentration and ligated with T4 ligase (NEB Cat# M0202S). Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (N | Full Paper |
2013 | Xing, F;Kobayashi, A;Okuda, H;Watabe, M;Pai, SK;Pandey, PR;Hirota, S;Wilber, A;Mo, YY;Moore, BE;Liu, W;Fukuda, K;Iiizumi, M;Sharma, S;Liu, Y;Wu, K;Peralta, E;Watabe, K; | Reactive astrocytes promote the metastatic growth of breast cancer stem-like cells by activating Notch signalling in brain | EMBO Mol Med | 23495140 | ... ocyte (UC1) was a kind gift from Dr Russell Piper (University of California-San Francisco). Primary human breast tumour cells which maintained in xenograft tumour of NOD/SCID mouse were obtained from Conversant Biologics, Inc. shRNA-expressing le | Full Paper |
2013 | Park, S;Balasubramaniam, V;Sastry, S; | Estimating pressure induced changes in vegetable tissue using in situ electrical conductivity measurement and instrumental analysis | Journal of Food Engineering | Pittsfield, MA, USA). The ends of the copper wires were soldered to the high pressure electrical feed through and electrically insulated using polyolefin heat shrink tubing (Q2-Z-1/8, Qualtek Electronics, Mentor, OH, USA). A type | Full Paper | |
2013 | Okuda, H;Xing, F;Pandey, PR;Sharma, S;Watabe, M;Pai, SK;Mo, YY;Iiizumi-Gairani, M;Hirota, S;Liu, Y;Wu, K;Pochampally, R;Watabe, K; | miR-7 suppresses brain metastasis of breast cancer stem-like cells by modulating KLF4 | Cancer Res. | 23384942 | mixture. Breast tumor cells that were directly transplanted to animal only one generation without in vitro culture were obtained from Conversant Biologics. Stem cell populations were isolated as described previously (14). Iso ;... odium pyruvate | Full Paper |
2013 | Sah, S;Chen, L;Houghton, J;Kemppainen, J;Marko, AC;Zeigler, R;Latham, GJ; | Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies | Genome Med | 24001039 | Seventy-six FFPE blocks containing thyroid tumor biopsies were purchased from Asterand (Detroit, MI, USA). The remaining 39 FFPE tissue specimens were processed from colorectal cancer resections purchased from Folio Biosciences (Columbus, OH, USA) [1 | Full Paper |
2013 | Soria, JC;Massard, C;Lazar, V;Ozoux, ML;Mery-Mignard, D;Deslandes, A;Tolcher, AW; | A dose finding, safety and pharmacokinetic study of AVE1642, an anti-insulin-like growth factor-1 receptor (IGF-1R/CD221) monoclonal antibody, administered as a single agent and in combination with docetaxel in patients with advanced solid tumours | Eur. J. Cancer | 23485230 | Tumour tissue: When possible, tumour tissue was collected before AVE1642 administration and at d21-d22 of cycle 1 to evaluate IGF-1R expression and signalling pathway markers, using validated immunohistochemistry assays, in Qualtek (USA) | Full Paper |
2013 | Rafaelsen, SH;Raeder, H;Fagerheim, AK;Knappskog, P;Carpenter, TO;Johansson, S;Bjerknes, R; | Exome sequencing reveals FAM20c mutations associated with fibroblast growth factor 23-related hypophosphatemia, dental anomalies, and ectopic calcification | J. Bone Miner. Res. | 23325605 | Genomic DNA was purified from blood using the QiaSymphony system (Qiagen, Hilden, Germany). Whole‐genome single‐nucleotide polymorphism (SNP) genotyping was performed with Genome‐Wide Human SNP 6.0 array (Affymetrix, Santa Clara, CA, USA). Whole‐exom | Full Paper |
2013 | Soler, VJ;Tran-Viet, KN;Galiacy, SD;Limviphuvadh, V;Klemm, TP;St Germain, E;Fournié, PR;Guillaud, C;Maurer-Stroh, S;Hawthorne, F;Suarez, C;Kantelip, B;Afshari, NA;Creveaux, I;Luo, X;Meng, W;Calvas, P;Cassagne, M;Arné, JL;Rozen, SG;Malecaze, F;Young, TL; | Whole exome sequencing identifies a mutation for a novel form of corneal intraepithelial dyskeratosis | J. Med. Genet. | 23349227 | Seven micrograms of DNA were submitted to Hudson Alpha Laboratories (Huntsville, Alabama, USA) (http://www.hudsonalpha. org/) for deep sequencing processing to achieve an average coverage of 50× read depth. The libraries were prepared according to ma | Full Paper |
2013 | Handorf, CR;Kulkarni, A;Grenert, JP;Weiss, LM;Rogers, WM;Kim, OS;Monzon, FA;Halks-Miller, M;Anderson, GG;Walker, MG;Pillai, R;Henner, WD; | A multicenter study directly comparing the diagnostic accuracy of gene expression profiling and immunohistochemistry for primary site identification in metastatic tumors | Am. J. Surg. Pathol. | 23648464 | meeting the criteria were obtained as archived formalin-fixed, paraffin-embedded specimens from 4 institutions: the University of Tennessee (UT); Virginia Commonwealth University (VCU); Indiana University-Purdue University, Indianapolis (IUPUI); and | Full Paper |
2013 | Majumdar, S;Gong, EM;Di Vizio, D;Dreyfuss, J;Degraff, DJ;Hager, MH;Park, PJ;Bellmunt, J;Matusik, RJ;Rosenberg, JE;Adam, RM; | Loss of Sh3gl2/endophilin A1 is a common event in urothelial carcinoma that promotes malignant behavior | Neoplasia | 23814487 | ILMN_1661491 [20]. Expression of Sh3gl2 protein was evaluated using commercially available bladder cancer tissue microarrays from either Folio Biosciences (Columbus, OH) or US Biomax, Inc (Rockville, MD). Specific immunohistochemical | Full Paper |
2013 | Bowman, GL;Dodge, HH;Mattek, N;Barbey, AK;Silbert, LC;Shinto, L;Howieson, DB;Kaye, JA;Quinn, JF; | Plasma omega-3 PUFA and white matter mediated executive decline in older adults | Front Aging Neurosci | 24379780 | Dr. Quinn has received honoraria for speaking from Pfizer, Novartis, and Forrest and for consulting from Phylogeny, Inc. Dr. Quinn is a co-inventor on a patent for the use of DHA for the treatment of Alzheimer's disease. Dr. Quinn receives compensati | Full Paper |
2013 | Allaire, PD;Seyed Sadr, M;Chaineau, M;Seyed Sadr, E;Konefal, S;Fotouhi, M;Maret, D;Ritter, B;Del Maestro, RF;McPherson, PS; | Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration | J. Cell. Sci. | 23264734 | Human brain tumor samples were obtained from the Montreal Neurological Institute Brain Tumor Tissue Bank. The experimental procedures were approved by the ethics committee of the Montreal Neurological Institute. The breast and squamous cell carcinoma | Full Paper |
2013 | Bohbot, JD;Durand, NF;Vinyard, BT;Dickens, JC; | Functional Development of the Octenol Response in Aedes aegypti | Front Physiol | 23471139 | We wish to thank the following individuals: Dr. Shawn E. Levy and Nripesh Prasad at the Genomic Services Lab at HudsonAlpha Institute for Biotechnology for Illumina sequencing and data analyses; Dr. Gary Bauchan (The Electron and Confocal Microscope | Full Paper |
2013 | Premkumar, DR;Jane, EP;Agostino, NR;DiDomenico, JD;Pollack, IF; | Bortezomib-induced sensitization of malignant human glioma cells to vorinostat-induced apoptosis depends on reactive oxygen species production, mitochondrial dysfunction, Noxa upregulation, Mcl-1 cleavage, and DNA damage | Mol. Carcinog. | 22086447 | time of tumor resection. We also obtained glioblastoma multiforme (GBM) primary cells from Conversant Biologics (Huntsville, AL), which were cultured in DMEM/F12 (Invitrogen, Carlsbad, CA). The established malignant glioma | Full Paper |
2013 | Kinder, M;Greenplate, AR;Grugan, KD;Soring, KL;Heeringa, KA;McCarthy, SG;Bannish, G;Perpetua, M;Lynch, F;Jordan, RE;Strohl, WR;Brezski, RJ; | Engineered protease-resistant antibodies with selectable cell-killing functions | J. Biol. Chem. | 23986451 | Immunohistochemistry. All immunohistochemistry was performed by QualTek Molecular Laboratories (Newtown, PA). Four-micrometer sections were dewaxed through four changes of xylene (5 min each) followed by a graded alcohol series to distilled water | Full Paper |
2013 | North, GB;Lynch, FH;Maharaj, FD;Phillips, CA;Woodside, WT; | Leaf hydraulic conductance for a tank bromeliad: axial and radial pathways for moving and conserving water | Front Plant Sci | 23596446 | Full Paper | |
2013 | Park, S;Balasubramaniam, V;Sastry, S;Lee, J; | Pressure–ohmic–thermal sterilization: A feasible approach for the inactivation of Bacillus amyloliquefaciens and Geobacillus stearothermophilus spores | Innovative Food Science & Emerging Technologies | Each soldered part was electrically insulated using heat shrink tubing (Q2-F-1/16-01-QB6IN- 36, Qualtek, Mentor, OH, USA) and epoxy (2-t ® epoxy clear, Devcon, Danvers, MA, USA) to prevent short circuits and current loss to the pressure-transmitting | Full Paper | |
2013 | Onsum, MD;Geretti, E;Paragas, V;Kudla, AJ;Moulis, SP;Luus, L;Wickham, TJ;McDonagh, CF;MacBeath, G;Hendriks, BS; | Single-cell quantitative HER2 measurement identifies heterogeneity and distinct subgroups within traditionally defined HER2-positive patients | Am. J. Pathol. | 24035511 | Patient Samples. Sections (5 μm thick) of a breast disease spectrum TMA were obtained from Folio Biosciences (Powell, OH) (catalog number ARY-HH0137). Duplicate 1-mm tissue cores from 48 patients were represented on the TMA | Full Paper |
2013 | Liu, S;Wang, X;Sun, F;Zhang, J;Feng, J;Liu, H;Rajendran, KV;Sun, L;Zhang, Y;Jiang, Y;Peatman, E;Kaltenboeck, L;Kucuktas, H;Liu, Z; | RNA-Seq reveals expression signatures of genes involved in oxygen transport, protein synthesis, folding, and degradation in response to heat stress in catfish | Physiol. Genomics | 23632418 | ;Login to your account. Institutional Login. Sign In / Register. PHYSIOLOGY.ORG Skip main navigationClose Drawer MenuOpen Drawer Menu Home: Journals: AJP-Cell; AJP-Endo; AJP-GI; AJP-Heart; AJP-Lung; AJP-Regu; AJP-Renal; ; For each group, equal | Full Paper |
2013 | Kauffman, AL;Gyurdieva, AV;Mabus, JR;Ferguson, C;Yan, Z;Hornby, PJ; | Alternative functional in vitro models of human intestinal epithelia | Front Pharmacol | 23847534 | n Majewski, Jason Ekert, and Kimberly Reese for help with iPSC maintenance culture. We also acknowledge the outstanding quality of immunohistochemistry performed by Steve Bernstein and Marko Sldanov, Qualtek Molecular Labs, Goleta, CA. References Al | Full Paper |
2013 | Lawley, B;Sims, IM;Tannock, GW; | Whole-transcriptome shotgun sequencing (RNA-seq) screen reveals upregulation of cellobiose and motility operons of Lactobacillus ruminis L5 during growth on tetrasaccharides derived from barley β-glucan | Appl. Environ. Microbiol. | 23851085 | Genomic comparisons were made in the expectation of understanding differential utilization of tetrasaccharides by L. ruminis strains. Paired-end reads were mapped to the publically available L. ruminis genomes: ATCC 25644 (did not utilize tetrasaccha | Full Paper |
2013 | Wang, R;Sun, L;Bao, L;Zhang, J;Jiang, Y;Yao, J;Song, L;Feng, J;Liu, S;Liu, Z; | Bulk segregant RNA-seq reveals expression and positional candidate genes and allele-specific expression for disease resistance against enteric septicemia of catfish | BMC Genomics | 24373586 | ;... erformed using KAPA Library Quant Kits (Kapa Biosystems, USA) and dilution, the library were clustered 3 in one lane and sequenced on a Hiseq 2500 instrument with 100 bp paired end (PE) reads at the HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2013 | Xu, J;Li, Q;Xu, L;Wang, S;Jiang, Y;Zhao, Z;Zhang, Y;Li, J;Dong, C;Xu, P;Sun, X; | Gene expression changes leading extreme alkaline tolerance in Amur ide (Leuciscus waleckii) inhabiting soda lake | BMC Genomics | 24094069 | ;... ). Equal amounts of the high quality RNA samples from each tissue were then pooled for RNA-Seq. cDNA library construction and sequencing RNA-Seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2013 | Rokyta, DR;Wray, KP;Margres, MJ; | The genesis of an exceptionally lethal venom in the timber rattlesnake (Crotalus horridus) revealed through comparative venom-gland transcriptomics | BMC Genomics | 23758969 | Sequencing and nonnormalized cDNA library preparation were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially as described by Mort | Full Paper |
2013 | Sugihara, K;Shibata, TK;Takata, K;Kimura, T;Kanayama, N;Williams, R;Hatakeyama, S;Akama, TO;Kuo, CW;Khoo, KH;Fukuda, MN; | Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans | FEBS Lett. | 23968720 | PolyLacs were collected and desalted using a Sephadex G-25 column and lyophilized. 2.7. Immunohistochemistry. Paraffin-embedded sections of human testis, epididymis, and ductus deferens tissues were obtained from Folio Biosciences (Columbus, OH) | Full Paper |
2013 | Gurudatta, BV;Yang, J;Van Bortle, K;Donlin-Asp, PG;Corces, VG; | Dynamic changes in the genomic localization of DNA replication-related element binding factor during the cell cycle | Cell Cycle | 23624840 | The reaction was stopped by adding glycine to a final concentration of 0.125 mM. Chromatin was prepared by shearing the chromatin using sonication. Chromatin was immunoprecipitated using antibodies to DREF,35,40 and the DNA was used for library prepa | Full Paper |
2013 | Haugarvoll, K;Johansson, S;Tzoulis, C;Haukanes, BI;Bredrup, C;Neckelmann, G;Boman, H;Knappskog, PM;Bindoff, LA; | MRI characterisation of adult onset alpha-methylacyl-coA racemase deficiency diagnosed by exome sequencing | Orphanet J Rare Dis | 23286897 | Genealogical data from the patients, local parish registers and population censuses were used. Whole genome, single nucleotide polymorphism (SNP) genotyping was performed utilizing the Genome-Wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA) and | Full Paper |
2013 | O'Donnell, P;Ferguson, J;Shyu, J;Current, R;Rehage, T;Tsai, J;Christensen, M;Tran, HB;Chien, SS;Shieh, F;Wei, W;Lawrence, HJ;Wu, L;Schilling, R;Bloom, K;Maltzman, W;Anderson, S;Soviero, S; | Analytic performance studies and clinical reproducibility of a real-time PCR assay for the detection of epidermal growth factor receptor gene mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancer | BMC Cancer | 23621958 | (Wilmington, DE, USA), Asterand,Inc. (Detroit, MI, USA), BioServe (Beltsville, MD, USA), Conversant (Huntsville,AL, USA), Cureline Inc. (South San Francisco, CA, USA), Cytomyx (Lexington, MA,USA), Discovery Life Sciences, Inc | Full Paper |
2013 | Peatman, E;Li, C;Peterson, BC;Straus, DL;Farmer, BD;Beck, BH; | Basal polarization of the mucosal compartment in Flavobacterium columnare susceptible and resistant channel catfish (Ictalurus punctatus) | Mol. Immunol. | 23895942 | ; preserving the replicate pools for replicated QPCR validation. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25 | Full Paper |
2013 | Sookruksawong, S;Sun, F;Liu, Z;Tassanakajon, A; | RNA-Seq analysis reveals genes associated with resistance to Taura syndrome virus (TSV) in the Pacific white shrimp Litopenaeus vannamei | Dev. Comp. Immunol. | 23921257 | RNA-Seq library preparation and sequencing was outsourced and performed in the Genomic Services Lab of the HudsonAlpha Biotechnology Institute (Huntsville, AL, USA) as previously described (Li et al., 2012b; Sun et al., 2012). The cDNA libraries of T | Full Paper |
2013 | Macias, JD;Gerkin, RD;Locke, D;Macias, MP; | Differential gene expression in cholesteatoma by DNA chip analysis | Laryngoscope | 23670528 | From the Macias Otology (J.D.M.), Biomedical Research Program, the EAR Foundation of Arizona (J.D.M., M.P.M.), and Scientific Services, Banner Health Research Institute (R.D.G.), Phoenix, Arizona, USA; and QualTek Molecular Laboratories (D.L.), Newto | Full Paper |
2013 | Infante, CR;Park, S;Mihala, AG;Kingsley, DM;Menke, DB; | Pitx1 broadly associates with limb enhancers and is enriched on hindlimb cis-regulatory elements | Dev. Biol. | 23201014 | Two independent biological replicates were used to generate two Illumina ChIP-Seq and two control libraries. All ChIP and input chromatin control libraries were produced using the Illumina ChIP-Seq DNA Sample Prep Kit (IP-102-1001) as directed by the | Full Paper |
2013 | Tzoulis, C;Johansson, S;Haukanes, BI;Boman, H;Knappskog, PM;Bindoff, LA; | Novel SACS mutations identified by whole exome sequencing in a norwegian family with autosomal recessive spastic ataxia of Charlevoix-Saguenay | PLoS ONE | 23785480 | Whole exome sequencing was performed at HudsonAlpha Institute for Biotechnology (Huntsville,AL) using Roche-NimbleGen Sequence Capture EZ Exome v2 kit and paired-end 100nt sequencing on the Illumina HiSeq [21]. | Full Paper |
2013 | Xu, J;Ji, P;Wang, B;Zhao, L;Wang, J;Zhao, Z;Zhang, Y;Li, J;Xu, P;Sun, X; | Transcriptome sequencing and analysis of wild Amur Ide (Leuciscus waleckii) inhabiting an extreme alkaline-saline lake reveals insights into stress adaptation | PLoS ONE | 23573207 | ;... lity RNA samples from each tissue were then pooled for cDNA synthesis and sequencing. cDNA Library Construction, Sequencing and Assembly RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2013 | Jiang, Y;Ninwichian, P;Liu, S;Zhang, J;Kucuktas, H;Sun, F;Kaltenboeck, L;Sun, L;Bao, L;Liu, Z; | Generation of physical map contig-specific sequences useful for whole genome sequence scaffolding | PLoS ONE | 24205335 | One microliter ligation product was used as template for amplification with the following reaction cocktail: 1 µl 10X buffer, 1 U polymerase, 0.4 µl MgCl2 [50 mM], 0.8 µl dNTPs [2.5 mM], 2µl [5 µM] in-house-designed primers. Reactions were conducted | Full Paper |
2013 | Liu, L;Li, C;Su, B;Beck, BH;Peatman, E; | Short-term feed deprivation alters immune status of surface mucosa in channel catfish (Ictalurus punctatus) | PLoS ONE | 24023952 | ;... ach condition, equal amounts of RNA from skin and gill were pooled to generate three pooled replicates for RNA-seq library construction. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2013 | Sun, F;Liu, S;Gao, X;Jiang, Y;Perera, D;Wang, X;Li, C;Sun, L;Zhang, J;Kaltenboeck, L;Dunham, R;Liu, Z; | Male-biased genes in catfish as revealed by RNA-Seq analysis of the testis transcriptome | PLoS ONE | 23874634 | ;... Bioanalysis chip. Equal amount of total RNA from catfish testis of each age was pooled together into only one sample for use in RNA-Seq. RNA-Seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2013 | Garofalo, M;Jeon, YJ;Nuovo, GJ;Middleton, J; | MiR-34a/c-dependent PDGFR-α/β downregulation inhibits tumorigenesis and enhances TRAIL-induced apoptosis in lung cancer | PloS one | The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: GJN is an employee of Phylogeny, Inc. There are no patents, products in development or marketed product | Full Paper | |
2013 | Lopez-Rios, F;Angulo, B;Gomez, B;Mair, D;Martinez, R;Conde, E;Shieh, F;Tsai, J;Vaks, J;Current, R;Lawrence, HJ;Gonzalez de Castro, D; | Comparison of molecular testing methods for the detection of EGFR mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancer | J. Clin. Pathol. | 23386666 | Six FFPET specimens were purchased from INDIVUMED Inc. (Kensington, Maryland, USA), Cureline, Inc. (South San Francisco,California, USA), Bioserve (Beltsville, Maryland, USA), and ConversantBio (Huntsville, Alabama, USA). Study design | Full Paper |
2013 | Lamerz, J;Friedlein, A;Soder, N;Cutler, P;Döbeli, H; | Determination of free desmosine in human plasma and its application in two experimental medicine studies | Anal. Biochem. | 23399390 | To validate the method, we used a plasma sample collection containing 30 patients with diagnosed COPD and 30 donors without a COPD diagnosis. This collection was obtained from Conversant Bio (Huntsville, AL, USA). Apart | Full Paper |
2013 | Pawar, H;Maharudraiah, J;Kashyap, MK;Sharma, J; | Downregulation of cornulin in esophageal squamous cell carcinoma | Acta histochemica | The ESCCs varied from tumor grade I to grade III from patients in the age group of 36-78 years. A second TMA set was obtained from FolioBio (Catalog no. ARY-HH0091, Powell, OH, USA) consisting of 40 ESCC cases with matched adjacent normal esophageal | Full Paper | |
2013 | Adorno, M;Sikandar, S;Mitra, SS;Kuo, A;Nicolis Di Robilant, B;Haro-Acosta, V;Ouadah, Y;Quarta, M;Rodriguez, J;Qian, D;Reddy, VM;Cheshier, S;Garner, CC;Clarke, MF; | Usp16 contributes to somatic stem-cell defects in Down's syndrome | Nature | 24025767 | Control bone marrow samples were obtained from AllCells, LLC. All samples were Control bone marrow samples were obtained from AllCells, LLC. Although we cannot unequivocally Save | Full Paper |
2013 | Amir, el-AD;Davis, KL;Tadmor, MD;Simonds, EF;Levine, JH;Bendall, SC;Shenfeld, DK;Krishnaswamy, S;Nolan, GP;Pe'er, D; | viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia | Nature biotechnology | 23685480 | Fresh, Ficoll-enriched human bone marrow was obtained from healthy donors from AllCells, Inc. (Emeryville, CA). Samples were obtained with informed consent in accordance with the Save | Full Paper |
2013 | Meng, X;Su, RJ;Baylink, DJ;Neises, A;Kiroyan, JB;Lee, WY;Payne, KJ;Gridley, DS;Wang, J;Lau, KH;Li, G;Zhang, XB; | Rapid and efficient reprogramming of human fetal and adult blood CD34+ cells into mesenchymal stem cells with a single factor | Cell research | 23478301 | University and written informed consent was obtained from all participants. CD34+ cells were purified with a CD34 Microbead Kit (Miltenyi Biotec, Auburn, CA, USA). Mobilized PB CD34+ cells were purchased from AllCells LLC (Emeryville, CA, USA) | Full Paper |
2013 | Yang, R;Liu, Y;Kelk, P;Qu, C;Akiyama, K;Chen, C;Atsuta, I;Chen, W;Zhou, Y;Shi, S; | A subset of IL-17(+) mesenchymal stem cells possesses anti-Candida albicans effect | Cell research | 23266891 | Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells (Emeryville, CA, USA). Human bone marrow progenitor cells were enriched using fresh human bone marrow by RosetteSep® Human Bone Marrow Progenitor | Full Paper |
2013 | Oshima, T;Sato, S;Kato, J;Ito, Y;Watanabe, T;Tsuji, I;Hori, A;Kurokawa, T;Kokubo, T; | Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers | Molecular cancer | 23758976 | Human peripheral blood mononuclear cells (PBMC) from AllCells were cultured in RPMI1640 medium containing 10% FBS, 0.1 nM human IL-2 (DIACLONE Research), and 55 μM 2- Save | Full Paper |
2013 | Takeishi, S;Matsumoto, A;Onoyama, I;Naka, K;Hirao, A;Nakayama, KI; | Ablation of Fbxw7 eliminates leukemia-initiating cells by preventing quiescence | Cancer cell | 23518349 | bone marrow mononuclear cells from treatment-naive patients in the chronic phase of CML and from healthy volunteers who had given informed consent were obtained from AllCells. Save | Full Paper |
2013 | Deckert, J;Park, PU;Chicklas, S;Yi, Y;Li, M;Lai, KC;Mayo, MF;Carrigan, CN;Erickson, HK;Pinkas, J;Lutz, RJ;Chittenden, T;Lambert, JM; | A novel anti-CD37 antibody-drug conjugate with multiple anti-tumor mechanisms for the treatment of B-cell malignancies | Blood | 24002446 | Frozen PBMCs from patients with CLL were obtained from AllCells (Emeryville, CA). For each sample, 5 × 10 5 cells were stained for indicated blood cell markers (Miltenyi) together with Save | Full Paper |
2013 | Cutler, C;Multani, P;Robbins, D;Kim, HT;Le, T;Hoggatt, J;Pelus, LM;Desponts, C;Chen, YB;Rezner, B;Armand, P;Koreth, J;Glotzbecker, B;Ho, VT;Alyea, E;Isom, M;Kao, G;Armant, M;Silberstein, L;Hu, P;Soiffer, RJ;Scadden, DT;Ritz, J;Goessling, W;North, TE;Mendlein, J;Ballen, K;Zon, LI;Antin, JH;Shoemaker, DD; | Prostaglandin-modulated umbilical cord blood hematopoietic stem cell transplantation | Blood | 23996087 | Primary cells Cryopreserved human UCB CD34+ primary cells were purchased from AllCells or Stem Cell Technologies (Vancouver, BC, Canada) | Full Paper |
2013 | Malinge, S;Chlon, T;Doré, LC;Ketterling, RP;Tallman, MS;Paietta, E;Gamis, AS;Taub, JW;Chou, ST;Weiss, MJ;Crispino, JD;Figueroa, ME; | Development of acute megakaryoblastic leukemia in Down syndrome is associated with sequential epigenetic changes | Blood | 23980066 | Michigan, and the University of Michigan. Eight specimens of human CD34+ bone marrow cells were provided by the Stem Cell and Xenograft Core Facility of the University of Pennsylvania or purchased from AllCells (Emeryville, CA). These studies were pe | Full Paper |
2013 | Kung Sutherland, MS;Walter, RB;Jeffrey, SC;Burke, PJ;Yu, C;Kostner, H;Stone, I;Ryan, MC;Sussman, D;Lyon, RP;Zeng, W;Harrington, KH;Klussman, K;Westendorf, L;Meyer, D;Bernstein, ID;Senter, PD;Benjamin, DR;Drachman, JG;McEarchern, JA; | SGN-CD33A: a novel CD33-targeting antibody-drug conjugate using a pyrrolobenzodiazepine dimer is active in models of drug-resistant AML | Blood | 23770776 | For the primary AML xenograft model, NOD/SCID/IL-2Rgnull mice (NSG; The Jackson Laboratory, Bar Harbor, ME) were irradiated with 1 Gy 1 day before intravenous injection of 7 × 105 primary leukemia cells from a patient with relapsed AML (06227; AllCel | Full Paper |
2013 | Kojima, K;Kornblau, SM;Ruvolo, V;Dilip, A;Duvvuri, S;Davis, RE;Zhang, M;Wang, Z;Coombes, KR;Zhang, N;Qiu, YH;Burks, JK;Kantarjian, H;Shacham, S;Kauffman, M;Andreeff, M; | Prognostic impact and targeting of CRM1 in acute myeloid leukemia | Blood | 23564911 | Normal marrow CD34 + cells from healthy donors aged 18 to 55 were obtained from AllCells (Emeryville, CA). The demographics and clinical characteristics of the patient population Save | Full Paper |
2013 | Lee, J;Shieh, JH;Zhang, J;Liu, L;Zhang, Y;Eom, JY;Morrone, G;Moore, MA;Zhou, P; | Improved ex vivo expansion of adult hematopoietic stem cells by overcoming CUL4-mediated degradation of HOXB4 | Blood | 23520338 | Each irradiated mouse (5 mice/group) was transplanted via tail vein injection with 3 × 105 CD34+ G-CSF-mobilized adult HSCs (AllCells, LLC, Emeryville, CA) treated with 2 rounds of purified recombinant wild-type or degradation-resistant HOXB4 protein | Full Paper |
2013 | Bhagat, TD;Zhou, L;Sokol, L;Kessel, R;Caceres, G;Gundabolu, K;Tamari, R;Gordon, S;Mantzaris, I;Jodlowski, T;Yu, Y;Jing, X;Polineni, R;Bhatia, K;Pellagatti, A;Boultwood, J;Kambhampati, S;Steidl, U;Stein, C;Ju, W;Liu, G;Kenny, P;List, A;Bitzer, M;Verma, A; | miR-21 mediates hematopoietic suppression in MDS by activating TGF-β signaling | Blood | 23390194 | Bone marrow CD34+ cells from various normal donors were also obtained from AllCells (Emeryville, CA). HS-5 cell line (CRL-11882) was purchased from ATCC (Manassas, VA). TGF-β1 was bought from R&D Systems (Minneapolis, MN). The phos-SMAD2 (S465/467) a | Full Paper |
2013 | Kumkhaek, C;Aerbajinai, W;Liu, W;Zhu, J;Uchida, N;Kurlander, R;Hsieh, MM;Tisdale, JF;Rodgers, GP; | MASL1 induces erythroid differentiation in human erythropoietin-dependent CD34+ cells through the Raf/MEK/ERK pathway | Blood | 23327923 | CD34+ cells were obtained from commercial sources (Lonza, Walkerville, MD, or AllCells, Emeryville, CA). Cells were thawed and washed into StemSpan serum-free expansion medium (SFEM) (StemCell Technologies, Vancouver, BC, Canada) and then seeded in S | Full Paper |
2013 | Wong, KY;Yim, RL;Kwong, YL;Leung, CY;Hui, PK;Cheung, F;Liang, R;Jin, DY;Chim, CS; | Epigenetic inactivation of the MIR129-2 in hematological malignancies | Journal of hematology & oncology | 23406679 | MIR124-1 and MIR203 were previously described [14,16]. Mononuclear cell DNA from 15 healthy donors [8 bone marrow, 3 peripheral blood, 3 CD19-sorted peripheral blood, and 1 CD138-sorted bone marrow (AllCells, CA, USA)] were used as negative control, | Full Paper |
2013 | Richardson, CL;Delehanty, LL;Bullock, GC;Rival, CM;Tung, KS;Kimpel, DL;Gardenghi, S;Rivella, S;Goldfarb, AN; | Isocitrate ameliorates anemia by suppressing the erythroid iron restriction response | The Journal of clinical investigation | 23863711 | The unique sensitivity of early red cell progenitors to iron deprivation, known as the erythroid iron restriction response, serves as a basis for human anemias globally. This response Save | Full Paper |
2013 | McBrian, MA;Behbahan, IS;Ferrari, R;Su, T;Huang, TW;Li, K;Hong, CS;Christofk, HR;Vogelauer, M;Seligson, DB;Kurdistani, SK; | Histone acetylation regulates intracellular pH | Molecular cell | 23201122 | H1 cells were maintained in mTeSR1 medium (STEMCELL Technologies #05850) on Matrigel. T cells were purchased from AllCells (PB009-1F) and were maintained in RPMI-1640 (ATCC #30-2001) with 10% FBS (Hyclone #SV3001403) that was heat inactivated by trea | Full Paper |
2013 | Kuroda, Y;Wakao, S;Kitada, M;Murakami, T;Nojima, M;Dezawa, M; | Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells | Nature protocols | 23787896 | For example, when we purchased bone marrow from ALLCELLS, which is imported from the United States to Japan and typically takes 5-7 d to arrive, the number of mononuclear cells Save | Full Paper |
2013 | Cheng, DT;Ma, C;Niewoehner, J;Dahl, M;Tsai, A;Zhang, J;Gonsiorek, W;Apparsundaram, S;Pashine, A;Ravindran, P;Jung, J;Hang, J;Allard, J;Bitter, H;Tribouley, C;Narula, S;Wilson, S;Fuentes, ME; | Thymic stromal lymphopoietin receptor blockade reduces allergic inflammation in a cynomolgus monkey model of asthma | The Journal of allergy and clinical immunology | 23810153 | Single donor human cord blood-derived CD34 + cells were purchased from AllCells (Emeryville, Calif). Cells were cultured for 8 weeks in serum-free complete medium (StemPro-34 Save | Full Paper |
2013 | Leone, E;Morelli, E;Di Martino, MT;Amodio, N;Foresta, U;Gullà, A;Rossi, M;Neri, A;Giordano, A;Munshi, NC;Anderson, KC;Tagliaferri, P;Tassone, P; | Targeting miR-21 inhibits in vitro and in vivo multiple myeloma cell growth | Clinical cancer research : an official journal of the American Association for Cancer Research | 23446999 | Imgenex) by standard procedures (37, 38). hBMSCs were obtained by long-term culture of bone marrow mononuclear cells (39). For coculture, 1 × 105 ppMM cells were seeded on 2 × 104 hBMSCs for 24 to 48 hours in 96-well plates. RNA samples of normal hea | Full Paper |
2013 | Kuhne, MR;Mulvey, T;Belanger, B;Chen, S;Pan, C;Chong, C;Cao, F;Niekro, W;Kempe, T;Henning, KA;Cohen, LJ;Korman, AJ;Cardarelli, PM; | BMS-936564/MDX-1338: a fully human anti-CXCR4 antibody induces apoptosis in vitro and shows antitumor activity in vivo in hematologic malignancies | Clinical cancer research : an official journal of the American Association for Cancer Research | 23213054 | Binding Buffer 10× concentrate, 7-aminoactinomycin D (7-AAD), and Annexin V-APC from BD Biosciences; phycoerythrin (PE)-conjugated goat anti-human antibody from Jackson ImmunoResearch (Cat. 109-116-098). AML peripheral blood mononuclear cells (PBMC) | Full Paper |
2013 | Corbin, AS;O'Hare, T;Gu, Z;Kraft, IL;Eiring, AM;Khorashad, JS;Pomicter, AD;Zhang, TY;Eide, CA;Manley, PW;Cortes, JE;Druker, BJ;Deininger, MW; | KIT signaling governs differential sensitivity of mature and primitive CML progenitors to tyrosine kinase inhibitors | Cancer research | 23887971 | Normal bone marrow mononuclear cells (MNC) were from AllCells. Cell selection was as described previously (details in Supplementary Methods; ref. 17). Save | Full Paper |
2013 | Ara, T;Nakata, R;Sheard, MA;Shimada, H;Buettner, R;Groshen, SG;Ji, L;Yu, H;Jove, R;Seeger, RC;DeClerck, YA; | Critical role of STAT3 in IL-6-mediated drug resistance in human neuroblastoma | Cancer research | 23633489 | AmpFISTR Identifier PCR kit and GeneMapper ID v. 3.2 (Applied Biosystems). Human BMMSC were purchased from AllCells LLC. Monocytes of normal healthy donors were obtained from peripheral blood and separated by Ficoll density gradient centrifugation us | Full Paper |
2013 | Wei, Y;Chen, R;Dimicoli, S;Bueso-Ramos, C;Neuberg, D;Pierce, S;Wang, H;Yang, H;Jia, Y;Zheng, H;Fang, Z;Nguyen, M;Ganan-Gomez, I;Ebert, B;Levine, R;Kantarjian, H;Garcia-Manero, G; | Global H3K4me3 genome mapping reveals alterations of innate immunity signaling and overexpression of JMJD3 in human myelodysplastic syndrome CD34+ cells | Leukemia | 23538751 | BMs from healthy individuals were obtained from AllCells (Emeryville, CA, USA). Isolation of CD34+ cells was performed using MicroBead Kit (Miltenyi, Bergisch Gladbach, Germany) Save | Full Paper |
2013 | Rio-Machin, A;Ferreira, BI;Henry, T;Gómez-López, G;Agirre, X;Alvarez, S;Rodriguez-Perales, S;Prosper, F;Calasanz, MJ;Martínez, J;Fonseca, R;Cigudosa, JC; | Downregulation of specific miRNAs in hyperdiploid multiple myeloma mimics the oncogenic effect of IgH translocations occurring in the non-hyperdiploid subtype | Leukemia | 23174883 | CD138-positive snap-frozen plasma cell pellets (ALLCELLS, Emeryville, CA, USA) were DNA from healthy CD138+ snap-frozen plasma cell pellets (ALLCELLS, Emeryville, CA, USA) Save | Full Paper |
2013 | Díaz-Beyá, M;Navarro, A;Ferrer, G;Díaz, T;Gel, B;Camós, M;Pratcorona, M;Torrebadell, M;Rozman, M;Colomer, D;Monzo, M;Esteve, J; | Acute myeloid leukemia with translocation (8;16)(p11;p13) and MYST3-CREBBP rearrangement harbors a distinctive microRNA signature targeting RET proto-oncogene | Leukemia | 23022987 | In addition, CD34+ cells from the bone marrow of three healthy donor subjects were purchased from AllCells (Emeryville, CA, USA). All patients provided their written informed consent Save | Full Paper |
2013 | Hayashi, Y;Hirai, H;Kamio, N;Yao, H;Yoshioka, S;Miura, Y;Ashihara, E;Fujiyama, Y;Tenen, DG;Maekawa, T; | C/EBPβ promotes BCR-ABL-mediated myeloid expansion and leukemic stem cell exhaustion | Leukemia | 22948537 | Frozen samples of human bone marrow cells from healthy donors or untreated CP-CML patients were purchased from AllCells LLC (Emeryville, CA, USA). In all cases, written informed Save;[方紘] 慢性期 CML 患者および健常人ドナーの骨髄細胞検体は AllCells 社より購入した. マウス 血幹細胞の細胞 で | Full Paper |
2013 | Lin, X;Rice, KL;Buzzai, M;Hexner, E;Costa, FF;Kilpivaara, O;Mullally, A;Soares, MB;Ebert, BL;Levine, R;Licht, JD; | miR-433 is aberrantly expressed in myeloproliferative neoplasms and suppresses hematopoietic cell growth and differentiation | Leukemia | 22864358 | Human CD34+ cells were obtained from adult bone marrow (AllCells, Emeryville, CA, USA) and cultured in StemSpan serum-free expansion media (SFEM; Stem Cell Technologies, Save | Full Paper |
2013 | Shin, JW;Spinler, KR;Swift, J;Chasis, JA;Mohandas, N;Discher, DE; | Lamins regulate cell trafficking and lineage maturation of adult human hematopoietic cells | Proceedings of the National Academy of Sciences of the United States of America | 24191023 | Commercially available primary human cells from marrow were obtained from either AllCells, Inc. (Emeryville, CA) or anonymous donors to the University of Pennsylvania Stem Cell Save | Full Paper |
2013 | Xie, J;Zhang, H;Yea, K;Lerner, RA; | Autocrine signaling based selection of combinatorial antibodies that transdifferentiate human stem cells | Proceedings of the National Academy of Sciences of the United States of America | 23613575 | Differentiation of CD34+ Cells in Culture. Fresh bone marrow CD34+ cells were purchased from Allcells (catalog no. ABM010). The purity is greater than 96% as determined by FACS. The cells were washed with SFEM (Stem Cell 09600) and kept in SFEM with | Full Paper |
2013 | Hong, KU;Li, QH;Guo, Y;Patton, NS;Moktar, A;Bhatnagar, A;Bolli, R; | A highly sensitive and accurate method to quantify absolute numbers of c-kit+ cardiac stem cells following transplantation in mice | Basic research in cardiology | 23549981 | Female human peripheral mononuclear cells (hPBMCs) were purchased from AllCells, LLC (Emeryville, CA). Aliquots of 105 hPBMCs (in 50 μl PBS) were stored at −20 °C until use. For isolation of genomic DNA, QIAamp DNA Mini Kit (Qiagen) was used accordin | Full Paper |
2013 | Ramakrishnan, P;Clark, PM;Mason, DE;Peters, EC;Hsieh-Wilson, LC;Baltimore, D; | Activation of the transcriptional function of the NF-κB protein c-Rel by O-GlcNAc glycosylation | Science signaling | 23982206 | Biotechnology. The anti-FLAG antibody was from Sigma. The anti-O-GlcNAc antibodies RL2 and CTD 110.6 were from Abcam and Covance, respectively. Cells Jurkat, Jurkat T-REx, Ramos, EL4, MEFs, and HEK 293T cells were grown as previously described (23). | Full Paper |
2013 | Isgro, J;Gupta, S;Jacek, E;Pavri, T;Duculan, R;Kim, M;Kirou, KA;Salmon, JE;Pernis, AB; | Enhanced rho-associated protein kinase activation in patients with systemic lupus erythematosus | Arthritis and rheumatism | 23508371 | Cryopreserved vials of human cord blood CD4+ T lymphocytes were obtained from Lonza Poietics, Immune Cell Systems, and AllCells, LLC. CD4+ T cell purity was 95%. Cells were thawed according to manufacturer’s instructions and cultured in LGM-3 (Lonza) | Full Paper |
2013 | Allakhverdi, Z;Comeau, MR;Armant, M;Agrawal, R;Woodfolk, JA;Sehmi, R;Howie, KJ;Gauvreau, GM;Delespesse, G; | Mast Cell-Activated Bone Marrow Mesenchymal Stromal Cells Regulate Proliferation and Lineage Commitment of CD34(+) Progenitor Cells | Frontiers in immunology | 24381572 | ibody were used at 10 μg/ml, as in our previous study (4). Primary human bone marrow-derived mesenchymal stromal cells Human BM-derived mesenchymal stromal cells were established from BM samples (AllCells, Emeryville, CA, USA) by culture in minimum | Full Paper |
2013 | Wu, Q;Wang, H;Zhao, X;Shi, Y;Jin, M;Wan, B;Xu, H;Cheng, Y;Ge, H;Zhang, Y; | Identification of G-protein-coupled receptor 120 as a tumor-promoting receptor that induces angiogenesis and migration in human colorectal carcinoma | Oncogene | 23851494 | ECMatrix (50 μl, Merck KGaA ) was distributed per well in a 96-well plate. HUVECs (ALLCELLS, Shanghai, China, 2 × 104 cells/well) suspended in 100 μl of medium supplemented with 0.5% FBS were added to each well and cultured in triplicate with 150 μl | Full Paper |
2013 | Li, C;Takahashi, C;Zhang, L;Huseni, M;Stankovich, B;Mashhedi, H;Lee, J;French, D;Anderson, JE;Kim, D;Howell, K;Brauer, MJ;Kowanetz, M;Yan, Y;Humke, E;Ebens, A;Hampton, G;Lackner, MR;Hegde, P;Jia, S; | Development of a robust flow cytometry-based pharmacodynamic assay to detect phospho-protein signals for phosphatidylinositol 3-kinase inhibitors in multiple myeloma | Journal of translational medicine | 23522020 | le myeloma Li Congfen 1 li.congfen@gene.com Takahashi Chikara 1 takahashi.chikara@gene.com Zhang Liangxuan 1 zhang.liangxuan@gene.com Huseni Mahrukh 1 mahrukhh@gene.com Stankovich Basha 2 bstankovich@allcells.com Mashhedi Haider 1 mashhedh@gene.com L | Full Paper |
2013 | Nazarov, C;Lo Surdo, J;Bauer, SR;Wei, CH; | Assessment of immunosuppressive activity of human mesenchymal stem cells using murine antigen specific CD4 and CD8 T cells in vitro | Stem cell research & therapy | 24406271 | by the Association for Assessment and Accreditation of Laboratory Animal Care International. All experiments were performed according to institutional guidelines. Cell culture hMSCs were purchased (AllCells, Emeryville, CA, USA) at passage one. Acco | Full Paper |
2013 | Ledford, KJ;Zeigler, F;Bartel, RL; | Ixmyelocel-T, an expanded multicellular therapy, contains a unique population of M2-like macrophages | Stem cell research & therapy | 24405629 | For the generation of M1 and M2 macrophages, human peripheral blood monocytes from healthy donors were purchased (AllCells LLC., Alameda, USA). The cells were polarized into Save | Full Paper |
2013 | Vanella, L;Sodhi, K;Kim, DH;Puri, N;Maheshwari, M;Hinds, TD;Bellner, L;Goldstein, D;Peterson, SJ;Shapiro, JI;Abraham, NG; | Increased heme-oxygenase 1 expression in mesenchymal stem cell-derived adipocytes decreases differentiation and lipid accumulation via upregulation of the canonical Wnt signaling cascade | Stem cell research & therapy | 23497794 | Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA, USA). After thawing, mononuclear cells were resuspended in an α-minimal essential Save | Full Paper |
2013 | McNamara, LA;Onafuwa-Nuga, A;Sebastian, NT;Riddell, J;Bixby, D;Collins, KL; | CD133+ hematopoietic progenitor cells harbor HIV genomes in a subset of optimally treated people with long-term viral suppression | The Journal of infectious diseases | 23554378 | and 7-aminoactinomycin D (7-AAD). Healthy donor BMMCs (AllCells) were stained with R-phycoerythrin-conjugated anti-CD133, R-phycoerythrin-Cy7-conjugated anti-CD34 (BD), and 7-AAD. Samples were analyzed on a BD FACSCanto | Full Paper |
2013 | Moy, LY;Jia, Y;Caniga, M;Lieber, G;Gil, M;Fernandez, X;Sirkowski, E;Miller, R;Alexander, JP;Lee, HH;Shin, JD;Ellis, JM;Chen, H;Wilhelm, A;Yu, H;Vincent, S;Chapman, RW;Kelly, N;Hickey, E;Abraham, WM;Northrup, A;Miller, T;Houshyar, H;Crackower, MA; | Inhibition of spleen tyrosine kinase attenuates allergen-mediated airway constriction | American journal of respiratory cell and molecular biology | 23889698 | + cells (5 x 10 6 /vial, AllCells) were thawed and added into 50 ml polypropylene conical tube with 100 µL of 1mg/ml DNase I (StemCell). 15.5 ml of pre-warmed Dulbecco's modified Save | Full Paper |
2013 | Ye, L;Muench, MO;Fusaki, N;Beyer, AI;Wang, J;Qi, Z;Yu, J;Kan, YW; | Blood cell-derived induced pluripotent stem cells free of reprogramming factors generated by Sendai viral vectors | Stem cells translational medicine | 23847002 | Frozen human peripheral blood mobilized CD34+ cells were purchased from AllCells (Emeryville, CA, http://www.allcells.com). After thawing, CD34+ cells were cultured with serum-free medium (SFM) as described by Ye and coworkers [24, 29], containing 50 | Full Paper |
2013 | Hall, SR;Jiang, Y;Leary, E;Yavanian, G;Eminli, S;O'Neill, DW;Marasco, WA; | Identification and isolation of small CD44-negative mesenchymal stem/progenitor cells from human bone marrow using elutriation and polychromatic flow cytometry | Stem cells translational medicine | 23847000 | Fresh, mobilized leukapheresis products were purchased from AllCells (Emeryville, CA, http://www.allcells.com) or collected from healthy volunteers at NeoStem Laboratory (Cambridge, MA, http://www.neostem.com) under an institutional review board-appr | Full Paper |
2013 | Mendelson, A;Cheung, Yk;Paluch, K;Chen, M;Kong, K;Tan, J;Dong, Z;Sia, SK;Mao, JJ; | Competitive stem cell recruitment by multiple cytotactic cues | Lab on a chip | 23364311 | Human bone marrow MSCs were isolated from fresh adult donor samples (AllCells, Emeryville, CA) using our prior methods. Briefly, the bone marrow sample was incubated with the Save | Full Paper |
2013 | Cairo, C;Sagnia, B;Cappelli, G;Colizzi, V;Leke, RG;Leke, RJ;Pauza, CD; | Human cord blood γδ T cells expressing public Vγ2 chains dominate the response to bisphosphonate plus interleukin-15 | Immunology | 23181340 | fraction of CBMC was reserved for cell culture and 0·5 × 107 to 1 × 107 were lysed for RNA extraction; remaining cells were frozen at 1 × 107 CBMC/ml, in 90% fetal bovine serum (FBS), 10% DMSO freezing medium. Some CBMC specimens were purchased from | Full Paper |
2013 | Kim, DH;Puri, N;Sodhi, K;Falck, JR;Abraham, NG;Shapiro, J;Schwartzman, ML; | Cyclooxygenase-2 dependent metabolism of 20-HETE increases adiposity and adipocyte enlargement in mesenchymal stem cell-derived adipocytes | Journal of lipid research | 23293373 | Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α-MEM, Save;ates: hypertension, the metabolic syndrome, and diabetes. MATERIALS | Full Paper |
2013 | Luo, Y;Kleiboeker, S;Deng, X;Qiu, J; | Human parvovirus B19 infection causes cell cycle arrest of human erythroid progenitors at late S phase that favors viral DNA replication | Journal of virology | 24049177 | Cells and virus. (i) CD36+ EPCs. Human bone marrow CD34+ hematopoietic stem/progenitor cells (HSCs) were positively isolated using a direct immunomagnetic CD34+ MicroBead labeling system and were purchased from AllCells, LLC (Alameda, CA; catalog no | Full Paper |
2013 | Shen, Y;Zeng, L;Zhu, A;Blanc, T;Patel, D;Pennello, A;Bari, A;Ng, S;Persaud, K;Kang, YK;Balderes, P;Surguladze, D;Hindi, S;Zhou, Q;Ludwig, DL;Snavely, M; | Removal of a C-terminal serine residue proximal to the inter-chain disulfide bond of a human IgG1 lambda light chain mediates enhanced antibody stability and antibody dependent cell-mediated cytotoxicity | mAbs | 23567210 | drogenase (LDH) activity from the lysed targeted cells (A431 transfected with receptor target X, A431x) was monitored in the presence freshly isolated normal peripheral blood mononuclear cells (PBMC, AllCells, PB002) upon the treatment with λ or λdS | Full Paper |
2013 | Zhao, Y;Wang, J;Wang, M;Sun, P;Chen, J;Jin, X;Zhang, H; | Activation of bone marrow-derived mesenchymal stromal cells-a new mechanism of defocused low-energy shock wave in regenerative medicine | Cytotherapy | 24199590 | Human umbilical vein endothelial cells (HUVEC) were purchased from Allcells (Shanghai, China) and maintained in EGM2 medium (CC-3162, Lonza, Basel, Switzerland). HUVEC Save | Full Paper |
2013 | Jones, M;Varella-Garcia, M;Skokan, M;Bryce, S;Schowinsky, J;Peters, R;Vang, B;Brecheisen, M;Startz, T;Frank, N;Nankervis, B; | Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System | Cytotherapy | 23992670 | Initially, six human bone marrow aspirates supplied by normal adult donors, who gave informed consent through a certified clinical collection facility (AllCells, Alameda, CA, USA), were Save | Full Paper |
2013 | Glen, KE;Workman, VL;Ahmed, F;Ratcliffe, E;Stacey, AJ;Thomas, RJ; | Production of erythrocytes from directly isolated or Delta1 Notch ligand expanded CD34+ hematopoietic progenitor cells: process characterization, monitoring and implications for manufacture | Cytotherapy | 23831361 | Background aims Economic ex vivo manufacture of erythrocytes at 10 12 cell doses requires an efficiently controlled bio-process capable of extensive proliferation and high terminal Save | Full Paper |
2013 | Song, L;Kauss, MA;Kopin, E;Chandra, M;Ul-Hasan, T;Miller, E;Jayandharan, GR;Rivers, AE;Aslanidi, GV;Ling, C;Li, B;Ma, W;Li, X;Andino, LM;Zhong, L;Tarantal, AF;Yoder, MC;Wong, KK;Tan, M;Chatterjee, S;Srivastava, A; | Optimizing the transduction efficiency of capsid-modified AAV6 serotype vectors in primary human hematopoietic stem cells in vitro and in a xenograft mouse model in vivo | Cytotherapy | 23830234 | CD34+ and umbilical cord blood CD34+ cells were either purchased from AllCells or obtained under a City of Hope Institutional Review Board approved protocol isolated as described by Chatterjee et al. [7], and maintained in either StemSpan SFEM medium | Full Paper |
2013 | Blackwood, E;Epler, J;Yen, I;Flagella, M;O'Brien, T;Evangelista, M;Schmidt, S;Xiao, Y;Choi, J;Kowanetz, K;Ramiscal, J;Wong, K;Jakubiak, D;Yee, S;Cain, G;Gazzard, L;Williams, K;Halladay, J;Jackson, PK;Malek, S; | Combination drug scheduling defines a "window of opportunity" for chemopotentiation of gemcitabine by an orally bioavailable, selective ChK1 inhibitor, GNE-900 | Molecular cancer therapeutics | 23873850 | For the bone marrow assay, fresh human bone marrow-derived cells were obtained from AllCells and plated at a density of 1 million cells/mL in Human Methylcellulose Complete Save | Full Paper |
2013 | Jekle, A;Yoon, J;Zuck, M;Najafi, R;Wang, L;Shiau, T;Francavilla, C;Rani, SA;Eitzinger, C;Nagl, M;Anderson, M;Debabov, D; | NVC-422 inactivates Staphylococcus aureus toxins | Antimicrobial agents and chemotherapy | 23208720 | Normal human peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA) and maintained in RPMI 1640 medium supplemented with 10% fetal bovine Save | Full Paper |
2013 | Winkler, DG;Faia, KL;DiNitto, JP;Ali, JA;White, KF;Brophy, EE;Pink, MM;Proctor, JL;Lussier, J;Martin, CM;Hoyt, JG;Tillotson, B;Murphy, EL;Lim, AR;Thomas, BD;Macdougall, JR;Ren, P;Liu, Y;Li, LS;Jessen, KA;Fritz, CC;Dunbar, JL;Porter, JR;Rommel, C;Palombella, VJ;Changelian, PS;Kutok, JL; | PI3K-δ and PI3K-γ inhibition by IPI-145 abrogates immune responses and suppresses activity in autoimmune and inflammatory disease models | Chemistry & biology | 24211136 | Primary Human B and T Cell Proliferation Assays Human peripheral blood CD19+ B cells and CD3+ T cells were purchased from Allcells and proliferation assays were conducted as described in Supplemental Experimental Procedures | Full Paper |
2013 | Liao, C;Hsu, J;Kim, Y;Hu, DQ;Xu, D;Zhang, J;Pashine, A;Menke, J;Whittard, T;Romero, N;Truitt, T;Slade, M;Lukacs, C;Hermann, J;Zhou, M;Lucas, M;Narula, S;DeMartino, J;Tan, SL; | Selective inhibition of spleen tyrosine kinase (SYK) with a novel orally bioavailable small molecule inhibitor, RO9021, impinges on various innate and adaptive immune responses: implications for SYK inhibitors in autoimmune disease therapy | Arthritis research & therapy | 24286216 | y kits (BD Biosciences). IgE-induced histamine release in human mast cells The method has been reported previously by our group [9]. Briefly, human cord blood-derived CD34+ hematopoietic stem cells (AllCells, Emeryville, CA, USA) were cultured in a | Full Paper |
2013 | Tsai, HC;Wu, R; | Cholera toxin directly enhances IL-17A production from human CD4+ T cells | Journal of immunology (Baltimore, Md. : 1950) | 24043897 | PBMCs were purchased from AllCells. According to the manufacturers' protocols, total CD4 + T cells were positively selected from PBMCs using magnetic CD4 MicroBeads (Miltenyi Save | Full Paper |
2013 | Sohn, SJ;Barrett, K;Van Abbema, A;Chang, C;Kohli, PB;Kanda, H;Smith, J;Lai, Y;Zhou, A;Zhang, B;Yang, W;Williams, K;Macleod, C;Hurley, CA;Kulagowski, JJ;Lewin-Koh, N;Dengler, HS;Johnson, AR;Ghilardi, N;Zak, M;Liang, J;Blair, WS;Magnuson, S;Wu, LC; | A restricted role for TYK2 catalytic activity in human cytokine responses revealed by novel TYK2-selective inhibitors | Journal of immunology (Baltimore, Md. : 1950) | 23894201 | IL-23 assay. IL-23 was obtained from R&D Systems and was used at a concentration that generated ∼50-90% of the maximal IL-23-induced signal in the assay (i.e., EC50-90 of IL-23). CD4 CD45RO+ T cells obtained from AllCells (Emeryville, CA) were cultur | Full Paper |
2013 | Yokley, BH;Selby, ST;Posch, PE; | A stimulation-dependent alternate core promoter links lymphotoxin α expression with TGF-β1 and fibroblast growth factor-7 signaling in primary human T cells | Journal of immunology (Baltimore, Md. : 1950) | 23547113 | Human T cells were purchased (Allcells, Emeryville, CA, USA) or were purified from PBMCs isolated from fresh whole blood with lymphocyte separation medium (Ficoll® and hypaque; Mediatech Inc, Manassas, VA, USA). Briefly, whole blood was brought to ro | Full Paper |
2013 | Fitzgerald, DC;Fonseca-Kelly, Z;Cullimore, ML;Safabakhsh, P;Saris, CJ;Zhang, GX;Rostami, A; | Independent and interdependent immunoregulatory effects of IL-27, IFN-β, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis | Journal of immunology (Baltimore, Md. : 1950) | 23455508 | PBMCs were isolated using Ficoll-Paque Plus density centrifugation; cord blood cells were obtained from AllCells. CD4 + and CD14 + cells were purified by immunomagnetic Save | Full Paper |
2013 | Heuck, CJ;Mehta, J;Bhagat, T;Gundabolu, K;Yu, Y;Khan, S;Chrysofakis, G;Schinke, C;Tariman, J;Vickrey, E;Pulliam, N;Nischal, S;Zhou, L;Bhattacharyya, S;Meagher, R;Hu, C;Maqbool, S;Suzuki, M;Parekh, S;Reu, F;Steidl, U;Greally, J;Verma, A;Singhal, SB; | Myeloma is characterized by stage-specific alterations in DNA methylation that occur early during myelomagenesis | Journal of immunology (Baltimore, Md. : 1950) | 23408834 | Eight samples from normal donors without known malignancies were obtained commercially from AllCells (Emeryville, CA). All normal donor samples had a purity of ≥85%. Save | Full Paper |
2013 | Jiang, Z;Hu, X;Yu, H;Xu, Y;Wang, L;Chen, H;Chen, H;Wu, R;Zhang, Z;Xiang, C;Webster, KA;Wang, JA; | Human endometrial stem cells confer enhanced myocardial salvage and regeneration by paracrine mechanisms | Journal of cellular and molecular medicine | 23837896 | the supplemental information. Cell sources Human BMMSCs from three donors were purchased from Cyagen Bioscience (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Human EnSCs from three | Full Paper |
2013 | He, X;Xu, Z;Wang, B;Zheng, Y;Gong, W;Huang, G;Zhang, L;Li, Y;He, F; | Upregulation of thrombomodulin expression by activation of farnesoid X receptor in vascular endothelial cells | European journal of pharmacology | 24041925 | The primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells, LLC (Shanghai, China) and were cultured in the medium supplied by AllCells with 10% Save | Full Paper |
2013 | Larusch, GA;Merkulova, A;Mahdi, F;Shariat-Madar, Z;Sitrin, RG;Cines, DB;Schmaier, AH; | Domain 2 of uPAR regulates single-chain urokinase-mediated angiogenesis through β1-integrin and VEGFR2 | American journal of physiology. Heart and circulatory physiology | 23709605 | HUVECs were cultured in 96-well microtiter plates on gelatin at 1 × 10 4 cells/well for 24 h in EGM from AllCells. Cells were then washed with serum-free medium without growth factor Save | Full Paper |
2013 | Chen, Y;Chitapanarux, T;Wu, J;Soon, RK;Melton, AC;Yee, HF; | Inducible NOS mediates CNP-induced relaxation of intestinal myofibroblasts | American journal of physiology. Gastrointestinal and liver physiology | 23348803 | NPR Western blots were performed by AllCells (Emeryville, CA) with the following antibodies: anti-mouse NPR-A (clone ab116680; Abcam, Cambridge, MA), anti-mouse NPR-B (clone Save | Full Paper |
2013 | Koestler, DC;Christensen, B;Karagas, MR;Marsit, CJ;Langevin, SM;Kelsey, KT;Wiencke, JK;Houseman, EA; | Blood-based profiles of DNA methylation predict the underlying distribution of cell types: a validation analysis | Epigenetics | 23903776 | As previously described,10,18 our reference set (S0) consisted of sorted, normal, human, peripheral blood leukocyte subtypes purchased from AllCells. Leukocytes were isolated from different, anonymous, non-diseased individuals’ whole-blood by magneti | Full Paper |
2013 | Harb, W;Fan, A;Tran, T;Danila, DC;Keys, D;Schwartz, M;Ionescu-Zanetti, C; | Mutational Analysis of Circulating Tumor Cells Using a Novel Microfluidic Collection Device and qPCR Assay | Translational oncology | 24151533 | Healthy donor peripheral blood was obtained from a commercial supplier (AllCells, Emeryville, CA) under Institutional Review Board (IRB) consent. Blood was drawn into EDTA tubes ( Save | Full Paper |
2013 | Shahaduzzaman, M;Golden, JE;Green, S;Gronda, AE;Adrien, E;Ahmed, A;Sanberg, PR;Bickford, PC;Gemma, C;Willing, AE; | A single administration of human umbilical cord blood T cells produces long-lasting effects in the aging hippocampus | Age (Dordrecht, Netherlands) | 23263793 | Cryopreserved HUCB MNC fraction and its component subfractions (CD3+, CD4+, CD8+, CD14+, and CD133+) were obtained from AllCells, LLC. HUCB cells were thawed in a 37 C Save | Full Paper |
2013 | de Lichtervelde, L;Boitano, AE;Wang, Y;Krastel, P;Petersen, F;Cooke, MP;Schultz, PG; | Eupalinilide E inhibits erythropoiesis and promotes the expansion of hematopoietic progenitor cells | ACS chemical biology | 23441826 | Human mPB CD34 + cells were purified from fresh human leukophoresed mobilized peripheral blood (AllCells) using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following Save | Full Paper |
2013 | Xiao, L;Hung, KC;Takahashi, TT;Joo, KI;Lim, M;Roberts, RW;Wang, P; | Antibody-mimetic ligand selected by mRNA display targets DC-SIGN for dendritic cell-directed antigen delivery | ACS chemical biology | 23427768 | Human PBMC-derived DCs were generated and purified as previously described, (46, 47) except that human PBMCs were from HLA-A2+ patients (AllCells, LLC.). Briefly, plastic-adherent cell fractions of human PBMCs were grown in RPMI 1640 with 10% heat-in | Full Paper |
2013 | Čokić, VP;Smith, RD;Biancotto, A;Noguchi, CT;Puri, RK;Schechter, AN; | Globin gene expression in correlation with G protein-related genes during erythroid differentiation | BMC genomics | 23425329 | isolation system (Miltenyi Biotec, Auburn, CA). Commercial FL- (Cambrex Bio Science, Inc., Walkersville, MD), CB-, BM- and granulocyte-colony stimulating factor (G-CSF) stimulated mobilized PB- (mPB, AllCells LLC, Berkeley, CA) derived CD34+ cells ar | Full Paper |
2013 | Musiychuk, K;Sivalenka, R;Jaje, J;Bi, H;Flores, R;Shaw, B;Jones, RM;Golovina, T;Schnipper, J;Khandker, L;Sun, R;Li, C;Kang, L;Voskinarian-Berse, V;Zhang, X;Streatfield, S;Hambor, J;Abbot, S;Yusibov, V; | Plant-produced human recombinant erythropoietic growth factors support erythroid differentiation in vitro | Stem cells and development | 23517237 | Umbilical cord blood-derived CD34 + cells were obtained from AllCells and maintained in the StemSpan Serum-Free Expansion Medium (StemCell Technologies) containing 1 μM Save | Full Paper |
2013 | Ramalingam, S;London, V;Kandavelou, K;Cebotaru, L;Guggino, W;Civin, C;Chandrasegaran, S; | Generation and genetic engineering of human induced pluripotent stem cells using designed zinc finger nucleases | Stem cells and development | 22931452 | Human primary CBMNCs were purchased from Allcells, Inc. The frozen CBMNCs were thawed and then cultured in the serum free medium (SFM) supplemented with cytokines and a Save | Full Paper |
2013 | Chen, J;Shi, ZD;Ji, X;Morales, J;Zhang, J;Kaur, N;Wang, S; | Enhanced osteogenesis of human mesenchymal stem cells by periodic heat shock in self-assembling peptide hydrogel | Tissue engineering. Part A | 23072422 | Human bone marrow (BM) from the iliac crest of a 27-year-old donor was purchased (AllCells LLC, Berkeley, CA). Mononuclear cells were enriched and retrieved with RosetteSep MSC enrichment cocktail (StemCell Technologies, Vancouver, Canada) according | Full Paper |
2013 | Wei, N;Yu, Y;Joshi, V;Schmidt, T;Qian, F;Salem, AK;Stanford, C;Hong, L; | Glucocorticoid receptor antagonist and siRNA prevent senescence of human bone marrow mesenchymal stromal cells in vitro | Cell and tissue research | 23963647 | Fresh human bone marrows, donated by total six healthy male and female donors from 22 to 43 years of age, were purchased from Allcells (Emeryville, CA). Mononuclear cells were isolated using density gradient centrifugation (Histopaque-1.077; Sigma, S | Full Paper |
2013 | Hutchinson, AT;Malik, A;Berkahn, MB;Agostino, M;To, J;Tacchi, JL;Djordjevic, SP;Turnbull, L;Whitchurch, CB;Edmundson, AB;Jones, DR;Raison, RL;Ramsland, PA; | Formation of assemblies on cell membranes by secreted proteins: molecular studies of free λ light chain aggregates found on the surface of myeloma cells | The Biochemical journal | 23822104 | We have described the presence of cell-membrane-associated κFLCs (free immunoglobulin light chains) on the surface of myeloma cells. Notably, the anti-κFLC mAb (monoclonal Save | Full Paper |
2013 | Choudhary, R;Baturin, D;Fosmire, S;Freed, B;Porter, CC; | Knockdown of HPRT for selection of genetically modified human hematopoietic progenitor cells | PloS one | 23555045 | Human umbilical cord blood samples unsuitable for clinical use were obtained from the ClinImmune Labs or were purchased from AllCells (Emeryville, CA). CD34 + cells were isolated Save | Full Paper |
2013 | Song, L;Li, X;Jayandharan, GR;Wang, Y;Aslanidi, GV;Ling, C;Zhong, L;Gao, G;Yoder, MC;Ling, C;Tan, M;Srivastava, A; | High-efficiency transduction of primary human hematopoietic stem cells and erythroid lineage-restricted expression by optimized AAV6 serotype vectors in vitro and in a murine xenograft model in vivo | PloS one | 23516552 | fetal bovine serum (FBS; Sigma, St. Louis, MO), 100 µg/ml of penicillin and 100 U/ml of streptomycin (Invitrogen, Grand Island, NY). Human cord blood CD34+ cells and CD36+ cells were purchased from AllCells (AllCells Technologies, Emeryville, CA) and | Full Paper |
2013 | Wang, T;Choi, E;Monaco, MC;Campanac, E;Medynets, M;Do, T;Rao, P;Johnson, KR;Elkahloun, AG;Von Geldern, G;Johnson, T;Subramaniam, S;Hoffman, DA;Hoffman, D;Major, E;Nath, A; | Derivation of neural stem cells from human adult peripheral CD34+ cells for an autologous model of neuroinflammation | PloS one | 24303066 | marker to detect mesenchymal cells, as CD29 and CD271, were used to stain the isolated CD34+ to further asses their purity. CD34+ cell culture and transfection Cord blood CD34+ cells (purchased from Allcells, Emeryville, California) or adult CD34+ c | Full Paper |
2013 | Xiao, F;Kim, YC;Wen, H;Luo, J;Chen, P;Cowan, K;Wang, SM; | The genome of polymorphonuclear neutrophils maintains normal coding sequences | PloS one | 24250807 | loid differentiation. Our study concludes that genomic DNA from myeloid lineage cells can be safely used in genetic studies. Methods Ethics Statement: The cells used for the study were obtained from AllCells LLC (http://www.allcells.com/, Emeryville | Full Paper |
2013 | Kornblau, SM;Qutub, A;Yao, H;York, H;Qiu, YH;Graber, D;Ravandi, F;Cortes, J;Andreeff, M;Zhang, N;Coombes, KR; | Proteomic profiling identifies distinct protein patterns in acute myelogenous leukemia CD34+CD38- stem-like cells | PloS one | 24223100 | tcome. This population lack samples from the 10-15% of AML cases that are CD34 negative. As normal controls, 1x106 CD34+ cells were collected from 10 different unstimulated normal bone marrow donors (AllCells, Emeryville, CA http://www.allcells.com/) | Full Paper |
2013 | Chai, ZT;Kong, J;Zhu, XD;Zhang, YY;Lu, L;Zhou, JM;Wang, LR;Zhang, KZ;Zhang, QB;Ao, JY;Wang, M;Wu, WZ;Wang, L;Tang, ZY;Sun, HC; | MicroRNA-26a inhibits angiogenesis by down-regulating VEGFA through the PIK3C2α/Akt/HIF-1α pathway in hepatocellular carcinoma | PloS one | 24194905 | ecco’s modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA) containing 10% fetal bovine serum (FBS) in a humidified incubator at 37°C with an atmosphere of 5% CO2. HUVECs were purchased from Allcells (Shanghai, China) and cultured in Endothelial | Full Paper |
2013 | Tarantal, AF;Lee, CC;Kukis, DL;Cherry, SR; | Radiolabeling human peripheral blood stem cells for positron emission tomography (PET) imaging in young rhesus monkeys | PloS one | 24098579 | CD34+ hPBSC were obtained from a commercial source (AllCells, Berkeley, CA) and cryopreserved using a controlled rate cryopreservation protocol in aliquots as previously reported [ Save | Full Paper |
2013 | DeBoever, C;Reid, EG;Smith, EN;Wang, X;Dumaop, W;Harismendy, O;Carson, D;Richman, D;Masliah, E;Frazer, KA; | Whole transcriptome sequencing enables discovery and analysis of viruses in archived primary central nervous system lymphomas | PloS one | 24023918 | To serve as a positive control a cord blood sample was purchased from AllCells (Emeryville, CA). Peripheral blood mononuclear cells (PBMC) were extracted from peripheral blood Save | Full Paper |
2013 | Dimicoli, S;Wei, Y;Bueso-Ramos, C;Yang, H;Dinardo, C;Jia, Y;Zheng, H;Fang, Z;Nguyen, M;Pierce, S;Chen, R;Wang, H;Wu, C;Garcia-Manero, G; | Overexpression of the toll-like receptor (TLR) signaling adaptor MYD88, but lack of genetic mutation, in myelodysplastic syndromes | PloS one | 23976989 | Bone marrows from healthy individuals were obtained from AllCells (Emeryville, CA). CD34+ cells were isolated from fresh bone marrow specimens of patients with MDS using Save | Full Paper |
2013 | Dolley-Sonneville, PJ;Romeo, LE;Melkoumian, ZK; | Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions | PloS one | 23940553 | Prior to the initiation of the multi-passage study we evaluated several plating densities based on recommendation of the manufacturer of hBM-MSCs used in the study (AllCells) and Save | Full Paper |
2013 | Holmberg, RC;Gindlesperger, A;Stokes, T;Lopez, D;Hyman, L;Freed, M;Belgrader, P;Harvey, J;Li, Z; | Akonni TruTip(®) and Qiagen(®) methods for extraction of fetal circulating DNA--evaluation by real-time and digital PCR | PloS one | 23936545 | ’s Circulating Nucleic Acid Kit. In order to determine the comparative extraction efficiency of the two methods on a large set of male fetal specimens, 19 maternal plasma samples were collected by AllCells (Emeryville, CA) or StemExpress (Placervill | Full Paper |
2013 | Hsiao, SC;Liu, H;Holstlaw, TA;Liu, C;Francis, CY;Francis, MB; | Real time assays for quantifying cytotoxicity with single cell resolution | PloS one | 23826123 | To each well were added 180 µL of freshly drawn human blood (AllCells, Inc.). After 16 h of incubation at 37C, the samples were visualized directly. Because the target cells are all Save | Full Paper |
2013 | Zhang, C;Wu, X;Zhang, M;Zhu, L;Zhao, R;Xu, D;Lin, Z;Liang, C;Chen, T;Chen, L;Ren, Y;Zhang, J;Qin, N;Zhang, X; | Small molecule R1498 as a well-tolerated and orally active kinase inhibitor for hepatocellular carcinoma and gastric cancer treatment via targeting angiogenesis and mitosis pathways | PloS one | 23755206 | nd by the vendors and subjected to in vitro assays between passages 8∼15, the cell lines for animal studies were between passages 5∼10. Human umbilical vein endothelial cell (HUVEC) obtained from Allcells (Emeryville, CA) was kept in EGM-2 (LONZA, Al | Full Paper |
2013 | Mendel, TA;Clabough, EB;Kao, DS;Demidova-Rice, TN;Durham, JT;Zotter, BC;Seaman, SA;Cronk, SM;Rakoczy, EP;Katz, AJ;Herman, IM;Peirce, SM;Yates, PA; | Pericytes derived from adipose-derived stem cells protect against retinal vasculopathy | PloS one | 23741506 | tient-derived lipoaspirate and further purified with serial passaging and sub-culturing, as we and others have previously shown [22]. Human bone marrow derived stem cells (hBMSCs) were purchased from Allcells (#MSC-001F). Bovine retinae and retinal m | Full Paper |
2013 | Su, RJ;Baylink, DJ;Neises, A;Kiroyan, JB;Meng, X;Payne, KJ;Tschudy-Seney, B;Duan, Y;Appleby, N;Kearns-Jonker, M;Gridley, DS;Wang, J;Lau, KH;Zhang, XB; | Efficient generation of integration-free ips cells from human adult peripheral blood using BCL-XL together with Yamanaka factors | PloS one | 23704989 | ied by MACS as described previously. [40] MNCs were obtained by standard density gradient centrifugation with Ficoll-Hypaque (1.077 g/ml) at room temperature. Some PBMNCs were directly purchased from AllCells (Emeryville, CA). The age of male and fem | Full Paper |
2013 | Evseenko, D;Latour, B;Richardson, W;Corselli, M;Sahaghian, A;Cardinal, S;Zhu, Y;Chan, R;Dunn, B;Crooks, GM; | Lysophosphatidic acid mediates myeloid differentiation within the human bone marrow microenvironment | PloS one | 23696850 | emical analyses. The murine stromal cell lines MS5 and OP9 (American Type Culture Collection (ATCC, Rockville, MD, USA) or primary human bone marrow derived mesenchymal stromal cells (passage 2-3) (AllCells Inc, Emeryville CA) were seeded into chambe | Full Paper |
2013 | Melton, AC;Melrose, J;Alajoki, L;Privat, S;Cho, H;Brown, N;Plavec, AM;Nguyen, D;Johnston, ED;Yang, J;Polokoff, MA;Plavec, I;Berg, EL;O'Mahony, A; | Regulation of IL-17A production is distinct from IL-17F in a primary human cell co-culture model of T cell-mediated B cell activation | PloS one | 23505568 | Frozen vials of positively selected primary normal human CD19+ B cells and negatively selected CD4+ T cells were obtained from AllCells (Emeryville, CA). PBMC were isolated from Save | Full Paper |
2013 | Angevine, K;Wuescher, L;Mensah-Osman, E; | Loss of menin mediated by endothelial cells treated with CoPP is associated with increased maturation of adipocytes | Adipocyte | 24052896 | , 10 mM l-glutamine, 0.75 units/mL heparin sulfate, 1 μg/mL hydrocortisone, 50 μg/mL ascorbic acid, and 2% fetal bovine serum. Bone marrow derived mesenchymal stem cells (MSCs) were purchased from AllCells and maintained at 37 °C and 5% CO2 in α-mem | Full Paper |
2013 | Omana-Zapata, I;Oreizy, F;Mosqueda, F;Salemenik, M;Lin, A;Wen, JL;Varro, R;Lee-Haynes, L;Segurado, OG; | Performance of a novel BD stem cell enumeration kit on two flow cytometry systems | International journal of laboratory hematology | 23190563 | Blood collection program and one frozen leukapheresis aliquot were enrolled for Linearity. Twenty-seven fresh leukapheresis aliquots from healthy mobilized adult donors were enrolled in the stability evaluation. All leukapheresis aliquots, fresh and | Full Paper |
2013 | Seki, K;Hisada, T;Kawata, T;Kamide, Y;Dobashi, K;Yamada, M;Mori, M;Okajima, F;Ishizuka, T; | Oxidative stress potentially enhances FcεRI-mediated leukotriene C4 release dependent on the late-phase increase of intracellular glutathione in mast cells | Biochemical and biophysical research communications | 23998930 | Human cord blood CD34 + cells were purchased from ALLCELLS (Emeryville, CA) and cultured in complete medium consisting of RPMI1640 (Life Technologies, Carlsbad, CA) Save | Full Paper |
2013 | Li, S;Garrett-Bakelman, FE;Akalin, A;Zumbo, P;Levine, R;To, BL;Lewis, ID;Brown, AL;D'Andrea, RJ;Melnick, A;Mason, CE; | An optimized algorithm for detecting and annotating regional differential methylation | BMC bioinformatics | 23735126 | Ten acute myeloid leukemia (AML) de-identified patient samples enriched for myeloblast cells and five normal bone marrow (NBM) samples (purchased from AllCells) were used in the Save | Full Paper |
2013 | Ohkawa, S;Kamei, N;Kamei, G;Shi, M;Adachi, N;Deie, M;Ochi, M; | Magnetic targeting of human peripheral blood CD133+ cells for skeletal muscle regeneration | Tissue engineering. Part C, Methods | 23298291 | )-mobilized human peripheral blood-derived-MNCs by using a direct immunomagnetic CD133 MicroBeads labeling and a magnetic cell sorting system, were purchased from AllCells( Save | Full Paper |
2013 | Kim, J;Zanjani, ED;Jeanblanc, CM;Goodrich, AD;Hematti, P; | Generation of CD34+ cells from human embryonic stem cells using a clinically applicable methodology and engraftment in the fetal sheep model | Experimental hematology | 23612043 | For generation of BM CD14 + cell-derived macrophages, CD14 + cells were isolated from BM aspirates of normal donors (AllCells) and harvested after 1 week (n = 6). For generation of Save | Full Paper |
2013 | Cherukuri, A;Patton, K;Gasser, RA;Zuo, F;Woo, J;Esser, MT;Tang, RS; | Adults 65 years old and older have reduced numbers of functional memory T cells to respiratory syncytial virus fusion protein | Clinical and vaccine immunology : CVI | 23239796 | A pooled human reference plasma standard (AllCells, LLC, Emeryville, CA) was included on each plate for comparison, and all plates were incubated for 1 h. A SulfoTag-labeled goat Save | Full Paper |
2013 | Kim, DH;Liu, J;Bhat, S;Benedict, G;Lecka-Czernik, B;Peterson, SJ;Ebraheim, NA;Heck, BE; | Peroxisome proliferator-activated receptor delta agonist attenuates nicotine suppression effect on human mesenchymal stem cell-derived osteogenesis and involves increased expression of heme oxygenase-1 | Journal of bone and mineral metabolism | 22945906 | Frozen bone marrow mononuclear cells were purchased from AllCells (Emeryville, CA, USA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential Save | Full Paper |
2013 | Li, L;Wang, Y;Chen, J;Cheng, B;Hu, J;Zhou, Y;Gao, X;Gao, L;Mei, X;Sun, M;Zhang, Z;Song, H; | An Engineered Arginase FC Protein Inhibits Tumor Growth In Vitro and In Vivo | Evidence-based complementary and alternative medicine : eCAM | 23737831 | umbilical cord vein were purchased from Allcells Inc. A confluent monolayer of synchronized HUVECS was scraped with a razor blade as described in [14]. After wounding, the cells Save | Full Paper |
2013 | Tummala, S;Titus, M;Wilson, L;Wang, C;Ciatto, C;Thill, G;Foster, D;Li, C;Szabo, Z;Guttman, A;Bettencourt, B;Jayaraman, M;Deroot, J;Kocisko, D;Pollard, S;Charisse, K;Kuchimanchi, S;Hinkle, G;Milstein, S;Meyers, R;Wu, SL;Karger, BL;Rossomando, A; | Evaluation of exogenous siRNA addition as a metabolic engineering tool for modifying biopharmaceuticals | Biotechnology progress | 23172735 | 12K media containing 10% fetal bovine serum. Fresh human normal PBMCs were purchased from AllCells (Emeryville, CA). Approximately 1 ×105 Jeko-1 cells were pre-incubated with RNAi-treated or control antibodies at varying concentrations ranging from 0 | Full Paper |
2013 | Rocha, LR;Veiga, DF;e Oliveira, PR;Song, EH;Ferreira, LM; | Health Service Quality Scale: Brazilian Portuguese translation, reliability and validity | BMC health services research | 23327598 | Business School, Avinguda de Pearson, 21, 08034, Barcelona, Spain Find articles by Paulo Rocha e Oliveira [https://pubmed.ncbi.nlm.nih.gov/?term=e%20Oliveira%20PR%5BAuthor%5D] ELAINE HORIBE SONG 4AllCells LLC, 5858 Horton Street, Emeryville, CA, 9 | Full Paper |
2013 | Ross, KM;McDonald-Jones, G;Miller, GE; | Oxytocin does not attenuate the ex vivo production of inflammatory cytokines by lipopolysaccharide-activated monocytes and macrophages from healthy male and female donors | Neuroimmunomodulation | 23899661 | For MP experiments, we purchased MO-derived, in vitro matured MPs from a cell supplier (AllCells, Emeryville, CA). All MPs used here were matured from a single donor's peripheral Save;Background: Oxytocin (OT) is a neuropeptide shown to attenuate i | Full Paper |
2013 | Wu, Y;Yang, Y;Yang, P;Gu, Y;Zhao, Z;Tan, L;Zhao, L;Tang, T;Li, Y; | The osteogenic differentiation of PDLSCs is mediated through MEK/ERK and p38 MAPK signalling under hypoxia | Archives of oral biology | 23806288 | The bone marrow stromal stem cells (BMSSCs) used as controls were obtained from a commercially available resource (AllCells LLC, Berkeley, CA, USA). Save | Full Paper |
2013 | Miller, P; | Stabilization of memory States by stochastic facilitating synapses | Journal of mathematical neuroscience | 24314108 | S is a scaled version of s, accounting for the totalfeedback conductance, S = W s , where W is the sum of connection strengths of allcells and held fixed when N is varied. Save | Full Paper |
2013 | Zhan, H;Cardozo, C;Yu, W;Wang, A;Moliterno, AR;Dang, CV;Spivak, JL; | MicroRNA deregulation in polycythemia vera and essential thrombocythemia patients | Blood cells, molecules & diseases | 23265742 | the peripheral blood CD34+ cells were 95% positive both for CD34 and CD38, were dimly positive for CD117, were CD41 negative, and were primarily in G0/G1 of the cell cycle [31]. Peripheral blood CD34+ cells from healthy controls were purchased from A | Full Paper |
2013 | Li, H;Xie, W;Gore, ER;Montoute, MN;Bee, WT;Zappacosta, F;Zeng, X;Wu, Z;Kallal, L;Ames, RS;Pope, AJ;Benowitz, A;Erickson-Miller, CL; | Development of phenotypic screening assays for γ-globin induction using primary human bone marrow day 7 erythroid progenitor cells | Journal of biomolecular screening | 24163393 | All cryopreserved human bone marrow CD34 + cells used here were obtained from AllCells (Emeryville, CA) and were generally from different donors. The CD34 + cells were cultured Save | Full Paper |
2013 | Schwimmer, LJ;Huang, B;Giang, H;Cotter, RL;Chemla-Vogel, DS;Dy, FV;Tam, EM;Zhang, F;Toy, P;Bohmann, DJ;Watson, SR;Beaber, JW;Reddy, N;Kuan, HF;Bedinger, DH;Rondon, IJ; | Discovery of diverse and functional antibodies from large human repertoire antibody libraries | Journal of immunological methods | 23454004 | The source RNA and cDNA used to amplify the V-genes was purchased from AllCells and Cureline. The E. coli strain TG1 (Lucigen) was used for all molecular cloning, phage Save | Full Paper |
2013 | Rosa, M;Pragasam, P;Saremian, J;Aoalin, A;Graf, W;Mohammadi, A; | The unsatisfactory ThinPrep® Pap Test™: analysis of technical aspects, most common causes, and recommendations for improvement | Diagnostic cytopathology | 22833404 | Fourth slide displays "halo effect" in which allcells are located at the periphery of the slide. This represents a technical preparation problem that can be resolved in the majority of cases Save | Full Paper |
2013 | Vainer, ED;Meir, K;Furman, M;Semenenko, I;Konikoff, F;Vainer, GW; | Characterization of novel CD55 isoforms expression in normal and neoplastic tissues | Tissue antigens | 23692281 | RNA was obtained from Ambion (Austin, TX), Asterand (Detroit, MI), AllCells (Emeryville, CA), Genomics Collaborative (Seracare; Cambridge, MA), Biochain (Newark, CA) and Ichilov medical center (Tel-aviv, Israel). CRC cell lines: colo205, DLD-1, HCT11 | Full Paper |
2013 | Muluk, NB;Arikan, OK;Atasoy, P;Kiliç, R;Yalçinozan, ET; | Inducible nitric oxide synthase (iNOS) in sinonasal polyp pathogenesis | B-ENT | 24273952 | the allcells group of inflammatory cells, there were PMNCs, MNCs, and fibroblasts. Higher iNOS expression in the allcells Thus, iNOS expression in the allcells group was mainly due to Save | Full Paper |
2013 | Wagner, LA;Wang, S;Wayner, EA;Christensen, C;Perkins, SJ;Ward, GW;Weiss, RB;Dunn, DM;Redd, MJ;Spangrude, GJ;Gleich, GJ; | Developing and mature human granulocytes express ELP 6 in the cytoplasm | Human antibodies | 24284306 | R&D Systems) or 2 U/ml epoietin-α [18]. Collection of de-identified UCMC was approved by the University of Utah’s Institutional Review Board. Bone marrow mononuclear cells (BMMC) were purchased from AllCells (Emeryville, CA) | Full Paper |
2013 | Köllmer, M;Buhrman, JS;Zhang, Y;Gemeinhart, RA; | Markers Are Shared Between Adipogenic and Osteogenic Differentiated Mesenchymal Stem Cells | Journal of developmental biology and tissue engineering | 24013643 | Human bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque TM PLUS solution followed by cell Save | Full Paper |
2013 | Hu, K;Slukvin, I; | Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors | Methods in molecular biology (Clifton, N.J.) | 23546755 | Human cord blood mononuclear cells (AllCells, CA, USA). 12. Human bone marrow mononuclear cells (AllCells, CA, USA). 13. Human bone marrow cells from patient with CML (AllCells, CA, USA). 14. Human cord blood CD34+ cells (AllCells, CA, USA). 15. | Full Paper |
2013 | Chu, EM;Tai, DC;Beer, JL;Hill, JS; | Macrophage heterogeneity and cholesterol homeostasis: classically-activated macrophages are associated with reduced cholesterol accumulation following treatment with oxidized LDL | Biochimica et biophysica acta | 23142249 | CD14 + human peripheral blood monocytes (AllCells) were cultured in RPMI 1640 complete media (Hyclone) containing 10% FBS (Hyclone), 2% sodium bicarbonate, 1% sodium Save | Full Paper |
2013 | Arora, M; | Cell Culture Media: A Review | Materials and Methods | ary cells. CELLS MEDIA Endothelial cells Human Endothelial-SFM (Life Technologies), Endothelial Basal Media, EndoGRO-LS Complete Media Kit (MilliporeSigma), HUVEC Basal Medium CB HUVEC (AllCells), Endothelial Cell Medium (ScienCell) | Full Paper | |
2013 | Mirshokraie, S;Mesbah, A;Pattabiraman, K; | Efficient JavaScript Mutation Testing | 2013 IEEE Sixth International Conference on Software Testing, Verification and Validation | function calls setup to set the dimension of all DOM elements with a class name of allCells; Assuming that the number of DOM elements with class name allCells is 9 (line 3 in Figure 1), Save | Full Paper | |
2013 | Fox, S;Adachi, T;Stillwell, W; | A QUINONE-ASSISTED PHOTOFORMATION OF ENERGY-RICH CHEMICAL BONDS | Solar Energy International Progress | In severalconferences , biochemists , chemicalengineers , andothershavediscusse dhow to industrializephotosyn thesis and how to industrializethe ATP cyclethatoperates in allcells . T Save | Full Paper | |
2013 | Margules, D;Flynn, J;Cooper, C; | CALCITONIN-LIKE PEPTIDE IN PRO-OPIOCORTIN: FUNCTIONAL ASPECTS | Advances in Endogenous and Exogenous Opioids | cells of the anterior pituitary and in allcells of the intermediate pituitary . EXCESS IN OBESE cells of the anterior pituitary and in allcells of the intermediate pituitary . EXCESS IN OBESE Save | Full Paper | |
2013 | Brodie, G; | CONSTRUCTED WETLANDS FOR TREATING ACID DRAINAGE AT TENNESSEE VALLEY AUTHORITY COAL FACILITIES | Constructed Wetlands in Water Pollution Control | S tagedtreatmentusingse veralcells may providea more efficientsystemsincec hemical and hydraulicoverloading of allcells would be minimized. Acid drainage treatment Water depth Save | Full Paper | |
2013 | Masuda, H;Asahara, T; | Isolation of Colony-Forming Endothelial Progenitor Cells | Manual of Research Techniques in Cardiovascular Medicine | approval of the institutional ethical committees of the Cord Blood and/or Clinical Investigation Committee. Alternatively, isolated MNCs, CD34+ , or CD133+ cells in UCB, PB, or BM can be purchased (e.g. ALLCELLSTM: http://www.allcells.com) | Full Paper | |
2013 | Drew, H; | Analysis of Macrophage- | Thesis | LPS, bovine CS and bovine aggrecan were purchased from SigmaAldrich (St. Louis, MO). Human monocytes (AllCells, LCC, Emeryville, CA) were cultured in RPMI-1640 with 10% fetal bovine serum. Bovine keratan sulfate proteoglycan (KSPG) was purchased f | Full Paper | |
2013 | Adams, GN; | Prolylcarboxypeptidase protects from vascular dysfunction and promotes vascular repair | Thesis | HUVEC were purchased from AllCells (Emeryville, CA) and grown on gelatin coated plates in CSC media (Cells Systems; Kirkland, WA). Cells were grown to near confluence in 6-well plates and transfected with Lipofectamine 2000 reagent (Invitrogen) in OP | Full Paper | |
2013 | Patel, VM; | Investigating apoptosis pathway in chronic lymphocytic leukemia: stromal influence and therapeutic activation | Thesis | All subjects signed written informed consent forms in accordance with the Declaration of Helsinki, and the laboratory protocols were approved by the institutional review board (IRB) at the University of Texas MD Anderson Cancer Center. Normal B‐lymph | Full Paper | |
2013 | Yi, S; | Green Manufacturing of Nanoparticles for Biomedical Applications | Thesis | Splenocytes, derived from C57BL/6 mice, were purchased from the Allcells Company (Emeryville, CA). HEPES, 1, 9-dimethyl-methylene blue (DMMB), chondroitin sulfate (CS), Sephadex G75, DEAE-cellulose and phosphate buffered saline (PBS) were purchased f | Full Paper | |
2013 | Adams, KJ; | Redirected T cell activity by high affinity TCR-ANTI-CD3 bispecific candidate therapeutics | Thesis | 2.2.2.3 Frozen peripheral blood mononuclear cells (PBMC) derived from multiple myeloma patients PBMC derived from multiple myeloma patients were purchased from AllCells as frozen aliquots, and were thawed and used on the day of the assay | Full Paper | |
2013 | PURWANTI, YIDA; | CANCER IMMUNOTHERAPY: TARGETED CELLULAR VEHICLE-MEDIATED IMMUNOGENE THERAPY AND DENDRITIC CELL-BASED VACCINE | Thesis | DCs and naïve T cells were derived from human peripheral blood mononuclear cells (PBMCs), both HLA-A2 positive and negative (AllCells, Stemcell Technologies Inc) based on previous studies 178. To generate DCs, 1×108 human PBMCs were seeded onto a T-1 | Full Paper | |
2013 | Kalim, KW; | The role of immunoproteasome subunit LMP7 in modulating T-helper cell differentiation and progression of autoimmune diseases | Thesis | cells were obtained from ATCC (Manassas, VA) and cultured in media recommended by the supplier. Human peripheral blood mononuclear cells (PBMC) from normal healthy volunteers were purchased from AllCells (Emeryville, CA) and those from individuals wi | Full Paper | |
2013 | Suknuntha, K; | An Induced Pluripotent Stem Cell-Based Platform for In Vitro Study of Chronic Myeloid Leukemia Stem Cell Development and Drug Resistance | Thesis | cells from CML patients in the chronic phase were purchased commercially (AllCells or in the chronic phase were purchased commercially (AllCells or Applied StemCells), or obtained Save | Full Paper | |
2013 | O'Huallachain, ME; | Genomic Variation in Somatic Human Tissues | Thesis | for a complexrearrangement in the PMP22 gene in an unaffected parent of a patient affect byCharcot-Marie-Tooth disease who had the same rearrangement throughout allcells. Save | Full Paper | |
2013 | Bhat, S; | Impact of nicotine and PPARδ-agonist on human mesenchymal stem cells | Thesis | An overview of all experiments performed in this thesis is provided on the previous page (see Figure 7). The overview includes type of experiment, treatments and assay/staining procedures conducted. Frozen (in 10%DMSO) bone marrow mononuclear cells ( | Full Paper | |
2013 | Kadam, S; | Global Landscape of DNA Methylation and Gene Expression in Hematopoiesis and Monocytic Differentiation | Thesis | Table 1: Information about the samples obtained from AllCells Inc; age, sex, ethnicity, lot number, catalog number, and location of cell (Bone Marrow and Peripheral Blood). RNA Expression Analysis in the Hematopoetic Lineage Even though DNA methylati | Full Paper | |
2013 | Kohn, LA; | Identification and Characterization of the Earliest Stages of Human Lymphoid Commitment | Thesis | Tissue Core of the University of California, Los Angeles, Cincinnati Children’s Hospital, or AllCells according to guidelines approved by Institutional Review Board of the University of California, Los Angeles. Samples were enriched for CD34+ cells | Full Paper | |
2013 | Haworth, KG; | Molecular Characterization of the HIV-1 Vpu Protein and Its Role in Antagonizing the Cellular Restriction Factor BST-2/Tetherin Both In Vitro and In Vivo | Thesis | Human peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA), depleted of CD8+ T cells using a positive selection CD8 microbead isolation kit (Miltenyi Biotech, Gladbach, Germany), and cultured in supplemented R10 medi | Full Paper | |
2013 | Cullimore, M; | Establishing a role for IL-27 in the amelioration of MS and EAE | Thesis | Whole venous blood was collected in EDTA-treated tubes from healthy donors after their informed consent. PBMCs were isolated using FiColl-Paque Plus™ density centrifugation while cord blood cells were obtained from AllCells. CD4+ /CD14+ cells were pu | Full Paper | |
2013 | Johnston, JM; | Optimization of a Lentiviral Vector for use in an HSC Gene Therapy Protocol for Hemophilia A | Thesis | CD34+ cells were purchased from AllCells (Emeryville, CA). Prior to arrival, CD34+ cells were mobilized from human bone marrow and isolated from the peripheral blood of 3 individual subjects. Flow cytometry was used to assess the purity of the sample | Full Paper | |
2013 | Xiao, L; | Dendritic cell-specific vaccine utilizing antibody-mimetic ligand and lentivector system | Thesis | Human PBMC-derived DCs were generated and purified as previously described,(Romani and others 1994; Sallusto and others 1994) except that human PBMCs were from HLA-A2+ patients (AllCells, LLC.). Briefly, plastic-adherent cell fractions of human PBMCs | Full Paper | |
2013 | Ji, T;Lin, C;Krill, LS;Eskander, R;Guo, Y;Zi, X;Hoang, BH; | Flavokawain B, a kava chalcone, inhibits growth of human osteosarcoma cells through G2/M cell cycle arrest and apoptosis | Molecular cancer | 23764122 | number of 2×104 cells were mixed with FKB or Adriamycin at concentration of 8.8 nM/ml, 17.6 nM/ml and 26.4 nM/ml, respectively. The mixture was cultured in 1 ml ColonyGel 1201 Mouse Base Medium (Reachbio) using a 6-well plate under standard culture | Full Paper |
2013 | Lee, YT;de Vasconcellos, JF;Yuan, J;Byrnes, C;Noh, SJ;Meier, ER;Kim, KS;Rabel, A;Kaushal, M;Muljo, SA;Miller, JL; | LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivo | Blood | 23798711 | All related studies were performed after human subjects review and National Institutes of Health Institutional Review Board approval. These studies were conducted in accordance with the Declaration of Helsinki. Human cord blood CD34+ cells were obtai | Full Paper |
2013 | Brandao, LN;Winges, A;Christoph, S;Sather, S;Migdall-Wilson, J;Schlegel, J;McGranahan, A;Gao, D;Liang, X;Deryckere, D;Graham, DK; | Inhibition of MerTK increases chemosensitivity and decreases oncogenic potential in T-cell acute lymphoblastic leukemia | Blood cancer journal | 23353780 | w cytometer and CXP data analysis software (Beckman Coulter). Methylcellulose assay Five hundred Jurkat parental and shRNA derivative cells were plated in methylcellulose-based medium (Cat no. 1101, Reachbio, Seattle, WA, USA) according to manufactu | Full Paper |
2013 | Grandi, FC;Rosser, JM;An, W; | LINE-1-derived poly(A) microsatellites undergo rapid shortening and create somatic and germline mosaicism in mice | Molecular biology and evolution | 23125228 | Cell and viability counts were performed using glacial acetic acid and trypan blue. Bone marrow cells were plated in ColonyGEL Complete Medium Without Epo (ReachBio LLC) at a density of 2 × 104 or 1 × 104 cells in 35 mm cell culture dishes and cultur | Full Paper |
2013 | Ulyanova, T;Jiang, Y;Padilla, SM;Papayannopoulou, T; | Erythroid cells generated in the absence of specific β1-integrin heterodimers accumulate reactive oxygen species at homeostasis and are unable to mount effective antioxidant defenses | Haematologica | 23812936 | CFU-C assays To quantify committed progenitors of all lineages, 50,000 BM cells, 500,000 splenic cells or 50-250 microliters of peripheral blood were cultured in semi-solid methylcellulose medium supplemented with cytokines (ReachBio) for seven days | Full Paper |
2013 | Zeiger, AS;Hinton, B;Van Vliet, KJ; | Why the dish makes a difference: quantitative comparison of polystyrene culture surfaces | Acta biomaterialia | 23454055 | Human bone-marrow-derived mesenchymal stromal or “stem” cells (hMSCs; ReachBio Seattle, WA) were expanded until passages 4-6, and plated onto P35 TCPS dishes at low density (∼5000 cells cm−2) to maintain subconfluent culture conditions | Full Paper |
2013 | Lee-Sherick, AB;Eisenman, KM;Sather, S;McGranahan, A;Armistead, PM;McGary, CS;Hunsucker, SA;Schlegel, J;Martinson, H;Cannon, C;Keating, AK;Earp, HS;Liang, X;DeRyckere, D;Graham, DK; | Aberrant Mer receptor tyrosine kinase expression contributes to leukemogenesis in acute myeloid leukemia | Oncogene | 23474756 | For methylcellulose colony forming assays, cells were plated in triplicate at a density of 500 cells/plate in Colony Gel Human Base Medium methylcellulose (Reach Bio, Seattle, WA, USA) with RPMI media containing 10% FBS added per manufacturer instruc | Full Paper |
2013 | Rossetto, CC;Tarrant-Elorza, M;Pari, GS; | Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells | PLoS pathogens | 23717203 | d, 10 ng/ml TPO, and 10 ng/ml TNF for three days, followed by the addition of 50 ng/ml lipopolysaccharide (LPS) (Sigma-Aldrich) for an additional four days. Human CD14 (+) cells purchased (Lonza and ReachBio) or isolated from cord blood were maintai | Full Paper |
2013 | Christoph, S;Deryckere, D;Schlegel, J;Frazer, JK;Batchelor, LA;Trakhimets, AY;Sather, S;Hunter, DM;Cummings, CT;Liu, J;Yang, C;Kireev, D;Simpson, C;Norris-Drouin, J;Hull-Ryde, EA;Janzen, WP;Johnson, GL;Wang, X;Frye, SV;Earp, HS;Graham, DK; | UNC569, a novel small-molecule mer inhibitor with efficacy against acute lymphoblastic leukemia in vitro and in vivo | Molecular cancer therapeutics | 23997116 | Jurkat or 697 cells were plated in methylcellulose-based medium (#1101; ReachBio) containing UNC569 or DMSO control according to the manufacturer's protocol (500 cells/mL Save | Full Paper |
2013 | Bausch, E;Kohlhof, H;Hamm, S;Krauss, R;Baumgartner, R;Sironi, L; | A novel microtubule inhibitor 4SC-207 with anti-proliferative activity in taxane-resistant cells | PloS one | 24324550 | Mouse femoral bone marrow cells derived from an 8 week old Balb/c mouse were qualified at ReachBio (in terms of progenitor frequency), and stored at -152C. Cells were prepared as Save;the absorbance at time zero (drug addition), Ti the absorbance a | Full Paper |
2013 | Weerasinghe, P;Hallock, S;Brown, RE;Loose, DS;Buja, LM; | A model for cardiomyocyte cell death: insights into mechanisms of oncosis | Experimental and molecular pathology | 22609242 | )-derived cardiomyocytes were purchased from Reach Bio LLC (Seattle, WA) and neonatal Sanguinarine was subsequently diluted with cardiomyocyte growth media (Reach Bio LLC, Save | Full Paper |
2013 | Maloney, JM;Lehnhardt, E;Long, AF;Van Vliet, KJ; | Mechanical fluidity of fully suspended biological cells | Biophysical journal | 24138852 | Primary adult human mesenchymal stem cells (hMSCs) were isolated from the bone marrow of four adult donors via Stem Cell Technologies (Vancouver, BC, Canada), ReachBio (Seattle, WA), or Lonza Group (Basel, Switzerland) | Full Paper |
2013 | Whitfield, MJ;Lee, WC;Van Vliet, KJ; | Onset of heterogeneity in culture-expanded bone marrow stromal cells | Stem cell research | 24103495 | BMSCs used for long-term imaging were purchased from ReachBio, and received at passage 1 (p1). Cells were seeded at ~ 2000 cells/cm 2 on tissue culture-treated polystyrene (BD Save | Full Paper |
2013 | Pierce, T; | Keeping Piracy to a Minimum | Appreneur | Two assay platforms, specifically designed for ADCs, have been developed by ReachBio as a result of increasing demand by industry for ways to assess the potential toxicity of these Save | Full Paper | |
2013 | Kennedy, JP; | A cellularized biomaterial model of cardiac fibroblasts for evaluation of fibroblast induced changes in stiffness | Thesis | Single donor human mesenchymal stem cells (MSC; ReachBio Seattle, WA) were passaged 2-3, and expression of CD29, CD73, CD105 CD34, and CD45 surface markers was evaluated by FACS (flow cytometry performed by manufacturer). U | Full Paper | |
2013 | Rosser, JM; | Investigating DNA methylation dynamics with transgenic LINE-1 mouse models | Thesis | Cell and viability counts were performed using glacial acetic acid and trypan blue. Bone marrow cells were plated in ColonyGEL Complete Medium Without Epo (ReachBio LLC) at a density | Full Paper | |
2012 | Xu, B;Ionita-Laza, I;Roos, JL;Boone, B;Woodrick, S;Sun, Y;Levy, S;Gogos, JA;Karayiorgou, M; | De novo gene mutations highlight patterns of genetic and neural complexity in schizophrenia | Nat. Genet. | 23042115 | We are enormously grateful to all the families who participated in this research. We thank H. Pretorius and nursing sisters R. van Wyk, C. Botha and H. van den Berg for their assistance with subject recruitment, family history assessments and diagnos | Full Paper |
2012 | Yang, J;Ramos, E;Corces, VG; | The BEAF-32 insulator coordinates genome organization and function during the evolution of Drosophila species | Genome Res. | 22895281 | on a Branson Sonifier 250 with output control set at 1.5. Libraries were prepared with the Illumina TruSeq DNA Sample Preparation Kit and sequenced at the HudsonAlpha Institute for Biotechnology. | Full Paper |
2012 | Van Bortle, K;Ramos, E;Takenaka, N;Yang, J;Wahi, JE;Corces, VG; | Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domains | Genome Res. | 22722341 | Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (NEB Cat# F-530L) and size selected for 200–300 bp by gel extraction. ChIP libraries were sequenced at the HudsonAlpha Institute for Biotechnology, using an Ill | Full Paper |
2012 | Kellner, WA;Ramos, E;Van Bortle, K;Takenaka, N;Corces, VG; | Genome-wide phosphoacetylation of histone H3 at Drosophila enhancers and promoters | Genome Res. | 22508764 | We thank Mattias Mannervik for the gift of CBP antibody and members of the Corces Lab for invaluable discussions. We also thank The Genomic Services Lab at the HudsonAlpha Institute for Biotechnology for their help in performing Illumina sequencing o | Full Paper |
2012 | Higgins, G;Roper, KM;Watson, IJ;Blackhall, FH;Rom, WN;Pass, HI;Ainscough, JF;Coverley, D; | Variant Ciz1 is a circulating biomarker for early-stage lung cancer | Proc. Natl. Acad. Sci. U.S.A. | 23074256 | ... anti-rabbit Alexa Fluor 488 (Invitrogen, Folio Bioscience). Subcellular fractionation was carried out as described (21). For immunohistochemistry, 2B was applied to SCLC tissue microarray ARY-HH0117 (Folio Biosciences) and visualized using Ultrav | Full Paper |
2012 | Fabbri, M;Paone, A;Calore, F;Galli, R;Gaudio, E;Santhanam, R;Lovat, F;Fadda, P;Mao, C;Nuovo, GJ;Zanesi, N;Crawford, M;Ozer, GH;Wernicke, D;Alder, H;Caligiuri, MA;Nana-Sinkam, P;Perrotti, D;Croce, CM; | MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory response | Proc. Natl. Acad. Sci. U.S.A. | 22753494 | We thank Phylogeny, Inc. for performing in situ hybridization in its entirety and locked nucleic acid in situ hybridization experiments and for supporting these experiment; Dr. Cecilia FernandezCymering and Dr. Stefano Volinia for supervision of stat | Full Paper |
2012 | Ren, H;Tan, ZP;Zhu, X;Crosby, K;Haack, H;Ren, JM;Beausoleil, S;Moritz, A;Innocenti, G;Rush, J;Zhang, Y;Zhou, XM;Gu, TL;Yang, YF;Comb, MJ; | Identification of anaplastic lymphoma kinase as a potential therapeutic target in ovarian cancer | Cancer Res. | 22570254 | Histologic typing and grading were carried out according to International Federation of Gynecology and Obstetrics (FIGO) guidelines. General pathologic information of the 69 ovarian tumor patients is listed in Supplementary Table S1. FFPE ovarian tum | Full Paper |
2012 | Polisetty, RV;Gautam, P;Sharma, R;Harsha, HC;Nair, SC;Gupta, MK;Uppin, MS;Challa, S;Puligopu, AK;Ankathi, P;Purohit, AK;Chandak, GR;Pandey, A;Sirdeshmukh, R; | LC-MS/MS analysis of differentially expressed glioblastoma membrane proteome reveals altered calcium signaling and other protein groups of regulatory functions | Mol. Cell Proteomics | 22219345 | ... tion [http://www.mcponline.org/cgi/content/full/M111.013565/DC1]. IMMUNOHISTOCHEMISTRY Formalin-fixed paraffin-embedded individual GBM tissue sections or commercially available tissue microarrays (Folio Biosciences, Columbus, OH) with 35 GBM t | Full Paper |
2012 | Gonzalez de Castro, D;Angulo, B;Gomez, B;Mair, D;Martinez, R;Suarez-Gauthier, A;Shieh, F;Velez, M;Brophy, VH;Lawrence, HJ;Lopez-Rios, F; | A comparison of three methods for detecting KRAS mutations in formalin-fixed colorectal cancer specimens | Br. J. Cancer | 22713664 | Materials: FFPET specimens from CRC tumours were purchased from US commercial vendors: Discovery Life Sciences, Inc. (Los Osos, CA, USA); BioServe (Beltsville, MD, USA); ProteoGenex (Culver City, CA, USA); CureLine, Inc | Full Paper |
2012 | Lenehan, PF;Boardman, LA;Riegert-Johnson, D;De Petris, G;Fry, DW;Ohrnberger, J;Heyman, ER;Gerard, B;Almal, AA;Worzel, WP; | Generation and external validation of a tumor-derived 5-gene prognostic signature for recurrence of lymph node-negative, invasive colorectal carcinoma | Cancer | 22605513 | (South San Francisco, Calif), Mayo Clinic (Rochester, Minn; Jacksonville, Fla; and Scottsdale, Ariz), Folio Biosciences (Columbus, Ohio), Trans‐Hit Biomarkers, Inc. (Montreal, Quebec, Canada), BioServe (Laurel, Md), and Amp‐Tec (Hamburg, Germany) | Full Paper |
2012 | Arrington, CB;Bleyl, SB;Matsunami, N;Bonnell, GD;Otterud, BE;Nielsen, DC;Stevens, J;Levy, S;Leppert, MF;Bowles, NE; | Exome analysis of a family with pleiotropic congenital heart disease | Circ Cardiovasc Genet | 22337856 | Exome sequencing (library construction, capture and sequencing) was carried out on 2 DNA samples (from patients III.3 and III.7) at the genomic service laboratory at HudsonAlpha Institute for Biotechnology (Huntsville, Alabama). Briefly, 3 to 5 µg of | Full Paper |
2012 | Dahmen, S;Haenni, M;Madec, JY; | IncI1/ST3 plasmids contribute to the dissemination of the blaCTX-M-1 gene in Escherichia coli from several animal species in France | J. Antimicrob. Chemother. | 22872449 | Characteristics of the eight CTX-M-1-producing E. coli isolates described in this study Strain Year District Animal Pathology b-Lactamases Additional resistancesa Phylogeny CTX-M-1 plasmid Inc group sequence type size (kb) 16170 2006 76 dog diarrhoea | Full Paper |
2012 | Fedyk, ER;Wyant, T;Yang, LL;Csizmadia, V;Burke, K;Yang, H;Kadambi, VJ; | Exclusive antagonism of the α4 β7 integrin by vedolizumab confirms the gut-selectivity of this pathway in primates | Inflamm. Bowel Dis. | 22419649 | Immunohistochemistry detecting the β7 integrin was conducted using a sheep antihuman β7 polyclonal antibody (R&D Systems, Minneapolis, MN) that does not compete with vedolizumab for binding to the α4β7 integrin expressed by leukocytes (data not shown | Full Paper |
2012 | Jenny, MJ;Aluru, N;Hahn, ME; | Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos | Toxicol. Appl. Pharmacol. | 22921993 | The same total RNA used for the array hybridizations was also used for high-throughput sequencing using the SOLiD technology. Prior to sequencing, the quality of the RNA and presence of the microRNA fraction was confirmed with the Agilent Small RNA k | Full Paper |
2012 | Liu, S;Zhang, Y;Zhou, Z;Waldbieser, G;Sun, F;Lu, J;Zhang, J;Jiang, Y;Zhang, H;Wang, X;Rajendran, KV;Khoo, L;Kucuktas, H;Peatman, E;Liu, Z; | Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote | BMC Genomics | 23127152 | ;... urer's protocol as in previous study [5]. Equal amount of total RNA from each tissue was combined and sent out for commercial sequencing. Illumina sequencing Sequencing was conducted commercially in HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2012 | Rokyta, DR;Lemmon, AR;Margres, MJ;Aronow, K; | The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus) | BMC Genomics | 23025625 | Sequencing and nonnormalized cDNA library preparation were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially as described by Mort | Full Paper |
2012 | Sun, F;Peatman, E;Li, C;Liu, S;Jiang, Y;Zhou, Z;Liu, Z; | Transcriptomic signatures of attachment, NF-κB suppression and IFN stimulation in the catfish gill following columnaris bacterial infection | Dev. Comp. Immunol. | ; for use in RNA-seq. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) as previously described by Li et al. (2012). cDNA libraries were prepared with | Full Paper | |
2012 | Li, C;Zhang, Y;Wang, R;Lu, J;Nandi, S;Mohanty, S;Terhune, J;Liu, Z;Peatman, E; | RNA-seq analysis of mucosal immune responses reveals signatures of intestinal barrier disruption and pathogen entry following Edwardsiella ictaluri infection in channel catfish, Ictalurus punctatus | Fish Shellfish Immunol. | 22366064 | ; replicate control pool was formed for use in RNA-seq. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25 ug of | Full Paper |
2012 | Nguyen, TH;Weber, W;Havari, E;Connors, T;Bagley, RG;McLaren, R;Nambiar, PR;Madden, SL;Teicher, BA;Roberts, B;Kaplan, J;Shankara, S; | Expression of TMPRSS4 in non-small cell lung cancer and its modulation by hypoxia | Int. J. Oncol. | 22692880 | Immunohistochemistry. A tissue array containing 101 formalin-fixed, paraffin-embedded tissue cores of lung SCC, adenocarcinomas, and normal non-neoplastic lung tissues from multiple donors was used (Phylogeny Inc., Columbus, OH) | Full Paper |
2012 | Gurudatta, BV;Ramos, E;Corces, VG; | The BEAF insulator regulates genes involved in cell polarity and neoplastic growth | Dev. Biol. | 22743648 | ChIP libraries were sequenced at the HudsonAlpha Institute for Biotechnology, using an Illumina HiSeq system. Sequences were mapped to the dm3 genome with Bowtie 0.12.3 (Langmead et al., 2009) using default settings. Peaks were then called with MACS | Full Paper |
2012 | Lee, S;Brophy, VH;Cao, J;Velez, M;Hoeppner, C;Soviero, S;Lawrence, HJ; | Analytical performance of a PCR assay for the detection of KRAS mutations (codons 12/13 and 61) in formalin-fixed paraffin-embedded tissue samples of colorectal carcinoma | Virchows Arch. | 22173329 | Material and methods. Materials. FFPET specimens from CRC tumors were purchased from US commercial vendors: Discovery Life Sciences, Inc. (Los Osos, CA, USA); BioServe (Beltsville, MD, USA); ProteoGenex (Culver City, CA, USA); CureLine, Inc | Full Paper |
2012 | Hughes, JA;Lynch, FC; | Transhepatic removal of an inferior vena cava filter | J Vasc Interv Radiol | 22720899 | Full Paper | |
2012 | Lynch, FC; | Modified loop snare technique for the removal of bard recovery, G2, G2 express, and eclipse inferior vena cava filters | J Vasc Interv Radiol | 22525025 | Full Paper | |
2012 | Vijay, K;Hughes, JA;Burdette, AS;Scorza, LB;Singh, H;Waybill, PN;Lynch, FC; | Fractured Bard Recovery, G2, and G2 express inferior vena cava filters: incidence, clinical consequences, and outcomes of removal attempts | J Vasc Interv Radiol | 22173108 | Full Paper | |
2012 | Mutka, SC;Green, LH;Verderber, EL;Richards, JP;Looker, DL;Chlipala, EA;Rosenthal, GJ; | ADH IB expression, but not ADH III, is decreased in human lung cancer | PLoS ONE | 23285246 | GSNOR immunohistochemistry. Immunohistochemical analysis for GSNOR was performed using formalin-fixed, paraffin-embedded human lung tissue microarrays (Folio Bioscience, Columbus, OH; #ARY-HH0178, #ARY-HH0176) | Full Paper |
2012 | Ueki, A;Shimizu, T;Masuda, K;Yamaguchi, SI;Ishikawa, T;Sugihara, E;Onishi, N;Kuninaka, S;Miyoshi, K;Muto, A;Toyama, Y;Banno, K;Aoki, D;Saya, H; | Up-regulation of Imp3 confers in vivo tumorigenicity on murine osteosarcoma cells | PLoS ONE | 23226335 | Zeiss). Human Osteosarcoma Tissue Array An array of human osteosarcoma specimens was obtained from Folio Biosciences (Powell, OH) and was subjected to immunohis- tochemical staining for IMP3 as described above ; Human Osteosarcoma Tissue Array. | Full Paper |
2012 | Xu, J;Ji, P;Zhao, Z;Zhang, Y;Feng, J;Wang, J;Li, J;Zhang, X;Zhao, L;Liu, G;Xu, P;Sun, X; | Genome-wide SNP discovery from transcriptome of four common carp strains | PLoS ONE | 23110192 | ;... e distribution of all samples were checked with Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, USA). cDNA Library Construction Illumina Sequencing Sequencing of 24 samples was conducted in HudsonAlpha Genomic Services Laboratory (Hunt | Full Paper |
2012 | Hedin, M;Starrett, J;Akhter, S;Schönhofer, AL;Shultz, JW; | Phylogenomic resolution of paleozoic divergences in harvestmen (Arachnida, Opiliones) via analysis of next-generation transcriptome data | PLoS ONE | 22936998 | Protolophus total RNA was shipped to Cofactor Genomics (www.cofactorgenomics.com), where a duplex-specific nuclease (DSN) normalized library was constructed, and 60 basepair (bp) paired-end reads were generated using Illumina GAII technology. All oth | Full Paper |
2012 | Johansson, S;Irgens, H;Chudasama, KK;Molnes, J;Aerts, J;Roque, FS;Jonassen, I;Levy, S;Lima, K;Knappskog, PM;Bell, GI;Molven, A;Njølstad, PR; | Exome sequencing and genetic testing for MODY | PLoS ONE | 22662265 | Targeted capture and massive parallel sequencing were performed at HudsonAlpha Institute for Biotechnology (Huntsville, AL) (File S1). In brief, SureSelect Human All Exon Kit (Agilent Technologies, Santa Clara, CA) was used for exome enrichment, and | Full Paper |
2012 | Myers, RB;Rwayitare, K;Richey, L;Lem, J;Castellot, JJ; | CCN5 Expression in mammals. III. Early embryonic mouse development | J Cell Commun Signal | 22926930 | earlier, at the 4-cell stage. Materials and methods. Immunohistochemistry. 5 μm frozen sagittal sections of C57BL/6 post-implantation embryos were obtained from Folio Bio (Columbus, OH). Sections were cleared with xylene | Full Paper |
2012 | Ding, D;Scott, NM;Thompson, EE;Chaiworapongsa, T;Torres, R;Billstrand, C;Murray, K;Dexheimer, PJ;Ismail, M;Kay, H;Levy, S;Romero, R;Lindheimer, MD;Nicolae, DL;Ober, C; | Increased protein-coding mutations in the mitochondrial genome of African American women with preeclampsia | Reprod Sci | 22902742 | Mitochondrial genome libraries were prepared by amplification of the complete mtDNA genome using 2 sets of overlapping primers. These fragments were pooled in molar equivalent amounts, sonicated to randomly fragment the DNA, and then prepared for seq | Full Paper |
2012 | Morimoto, M;Souich, Cd;Trinh, J;McLarren, KW;Boerkoel, CF;Hendson, G; | Expression profile of NSDHL in human peripheral tissues | J. Mol. Histol. | 22113624 | This construct was cut at unique NotI and SpeI sites flanking the Nsdhl insert and transcribed by T3 and T7 RNA polymerases to yield antisense and sense in situ hybridization probes, respectively. In situ hybridization was performed by Phylogeny, Inc | Full Paper |
2012 | Wang, R;Li, C;Stoeckel, J;Moyer, G;Liu, Z;Peatman, E; | Rapid development of molecular resources for a freshwater mussel, Villosa lienosa (Bivalvia:Unionidae), using an RNA-seq-based approach | Freshwater Science | After quantitation with KAPA Library Quant Kits (Kapa Biosystems, Woburn, Massachusetts) and dilution, the libraries were clustered 4 per lane and sequenced on a HiSeq 2000 instrument with 100-bp PE reads at the HudsonAlpha Genomic Services Laborator | Full Paper | |
2012 | Kishida, M;Ishige, K;Horibe, T;Tada, N;Koibuchi, N;Shoda, J;Kita, K;Kawakami, K; | Orexin 2 receptor as a potential target for immunotoxin and antibody-drug conjugate cancer therapy | Oncol Lett | 22740944 | Materials and methods. Materials. Hepatocellular carcinoma tissue array, ARY- HH0075 was purchased from Folio Biosciences (Columbus, OH, USA). Digestive system disease tissue array, DID381 was purchased from US Biomax (Rockville, MD, USA) | Full Paper |
2012 | Kentsis, A;Reed, C;Rice, KL;Sanda, T;Rodig, SJ;Tholouli, E;Christie, A;Valk, PJ;Delwel, R;Ngo, V;Kutok, JL;Dahlberg, SE;Moreau, LA;Byers, RJ;Christensen, JG;Vande Woude, G;Licht, JD;Kung, AL;Staudt, LM;Look, AT; | Autocrine activation of the MET receptor tyrosine kinase in acute myeloid leukemia | Nature medicine | 22683780 | This report identifies upregulation of HGF as an autocrine growth pathway in several subsets of AML. Ligand-dependent activation of MET represents a new oncogenic stimulus, and the Save | Full Paper |
2012 | Schenk, T;Chen, WC;Göllner, S;Howell, L;Jin, L;Hebestreit, K;Klein, HU;Popescu, AC;Burnett, A;Mills, K;Casero, RA;Marton, L;Woster, P;Minden, MD;Dugas, M;Wang, JC;Dick, JE;Müller-Tidow, C;Petrie, K;Zelent, A; | Inhibition of the LSD1 (KDM1A) demethylase reactivates the all-trans-retinoic acid differentiation pathway in acute myeloid leukemia | Nature medicine | 22406747 | We grew normal bone marrow mononuclear cells (AllCells, ABM011F) in IMDM supplemented with 20 ng ml −1 SCF, 10 ng ml −1 each of IL-3, IL-6 and fms-related tyrosine kinase 3 ( Save | Full Paper |
2012 | Zhang, C;Srinivasan, Y;Arlow, DH;Fung, JJ;Palmer, D;Zheng, Y;Green, HF;Pandey, A;Dror, RO;Shaw, DE;Weis, WI;Coughlin, SR;Kobilka, BK; | High-resolution crystal structure of human protease-activated receptor 1 | Nature | 23222541 | In brief, acid-citrate-dextrose anti-coagulated human blood samples (60 ml per donor) were obtained from AllCells. Blood was centrifuged without braking at 250g at 37 C for 15 min. Save | Full Paper |
2012 | Sasaki, M;Knobbe, CB;Munger, JC;Lind, EF;Brenner, D;Brüstle, A;Harris, IS;Holmes, R;Wakeham, A;Haight, J;You-Ten, A;Li, WY;Schalm, S;Su, SM;Virtanen, C;Reifenberger, G;Ohashi, PS;Barber, DL;Figueroa, ME;Melnick, A;Zúñiga-Pflücker, JC;Mak, TW; | IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigenetics | Nature | 22763442 | Two normal CD34 + bone marrow control samples were purchased from AllCells. Institutional review board approval was obtained at Weill Cornell Medical Center and this study was Save | Full Paper |
2012 | Kohn, LA;Hao, QL;Sasidharan, R;Parekh, C;Ge, S;Zhu, Y;Mikkola, HK;Crooks, GM; | Lymphoid priming in human bone marrow begins before expression of CD10 with upregulation of L-selectin | Nature immunology | 22941246 | esis in disease states. METHODS Bone marrow cell isolation Normal human BM and thymic cells were obtained from healthy donors via the UCLA Pathology Tissue Core, Cincinnati Children’s Hospital, or ALLCELLS according to guidelines approved by UCLA In | Full Paper |
2012 | Zhu, X;Wang, Y;Pi, W;Liu, H;Wickrema, A;Tuan, D; | NF-Y recruits both transcription activator and repressor to modulate tissue- and developmental stage-specific expression of human γ-globin gene | PloS one | 23071749 | The human cells were obtained commercially from AllCells and processed with protocols approved by Human Assurance Committee of Georgia Health Sciences University (HAC file # Save | Full Paper |
2012 | Hur, EM;Patel, SN;Shimizu, S;Rao, DS;Gnanapragasam, PN;An, DS;Yang, L;Baltimore, D; | Inhibitory effect of HIV-specific neutralizing IgA on mucosal transmission of HIV in humanized mice | Blood | 23065154 | intraperitoneally with 3 × 10 6 human PBMCs (AllCells) that were activated by PHA (5 μg/mL) NSG mice were transplanted intraperitoneally with 3 × 10 6 human PBMCs (AllCells) that Save | Full Paper |
2012 | Lu, M;Wang, X;Li, Y;Tripodi, J;Mosoyan, G;Mascarenhas, J;Kremyanskaya, M;Najfeld, V;Hoffman, R; | Combination treatment in vitro with Nutlin, a small-molecule antagonist of MDM2, and pegylated interferon-α 2a specifically targets JAK2V617F-positive polycythemia vera cells | Blood | 22872685 | Fresh normal human bone marrow CD34+ cells were purchased from ALLCELLS. The characteristics of the PV patients studied are shown in supplemental Table 1 (available on the Blood Web site; see the Supplemental Materials link at the top of the online a | Full Paper |
2012 | Ndhlovu, LC;Lopez-Vergès, S;Barbour, JD;Jones, RB;Jha, AR;Long, BR;Schoeffler, EC;Fujita, T;Nixon, DF;Lanier, LL; | Tim-3 marks human natural killer cell maturation and suppresses cell-mediated cytotoxicity | Blood | 22383801 | Peripheral blood mononuclear cells (PBMCs) of healthy individuals were obtained from the Stanford Blood Bank. Cord blood PMBCs were obtained from AllCells. The University of California, San Francisco and the University of Hawaii institutional review | Full Paper |
2012 | Wey, S;Luo, B;Tseng, CC;Ni, M;Zhou, H;Fu, Y;Bhojwani, D;Carroll, WL;Lee, AS; | Inducible knockout of GRP78/BiP in the hematopoietic system suppresses Pten-null leukemogenesis and AKT oncogenic signaling | Blood | 21937694 | The normal peripheral blood mononuclear cells were purchased from ALLCELLS (catalog #PB003F). The ethical use of the human tissues for research was approved by the University Save | Full Paper |
2012 | Cheng, L;Hansen, NF;Zhao, L;Du, Y;Zou, C;Donovan, FX;Chou, BK;Zhou, G;Li, S;Dowey, SN;Ye, Z;NISC Comparative Sequencing Program, ;Chandrasekharappa, SC;Yang, H;Mullikin, JC;Liu, PP; | Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by nonintegrating plasmid expression | Cell stem cell | 22385660 | blood of anonymous donors were collected and processed at AllCells, LLC (Emeryville, CA). The by Institutional Review Board (IRB) at AllCells. Use of anonymous human samples for Save | Full Paper |
2012 | Smith, MR;Atkinson, P;White, D;Piersma, T;Gutierrez, G;Rossini, G;Desai, S;Wellinghoff, S;Yu, H;Cheng, X; | Design and assessment of a wrapped cylindrical Ca-P AZ31 Mg alloy for critical-size ulna defect repair | Journal of biomedical materials research. Part B, Applied biomaterials | 22102519 | AZ31 Mg alloy cupons were prepared as described above and sterilized using ethylene oxide gas. Two cell migration tests were performed: indirect and direct. Frozen human MSCs (AllCells, LLC, Emeryville, CA) were thawed, spun down, and resuspended in | Full Paper |
2012 | Muthuswamy, R;Berk, E;Junecko, BF;Zeh, HJ;Zureikat, AH;Normolle, D;Luong, TM;Reinhart, TA;Bartlett, DL;Kalinski, P; | NF-κB hyperactivation in tumor tissues allows tumor-selective reprogramming of the chemokine microenvironment to enhance the recruitment of cytolytic T effector cells | Cancer research | 22593190 | vein endothelial cells (HUVEC; AllCells) were cultured in HUVEC complete media (Basal media supplemented with HUVEC stimulatory supplement; AllCells). All cell lines were washed Save | Full Paper |
2012 | Capozza, F;Trimmer, C;Castello-Cros, R;Katiyar, S;Whitaker-Menezes, D;Follenzi, A;Crosariol, M;Llaverias, G;Sotgia, F;Pestell, RG;Lisanti, MP; | Genetic ablation of Cav1 differentially affects melanoma tumor growth and metastasis in mice: role of Cav1 in Shh heterotypic signaling and transendothelial migration | Cancer research | 22396494 | B16F10 and A-375 were from ATCC, while human immortalized dermal foreskin fibroblasts (hTBJ1) were originally from Clontech. Human Umbilical Vein Endothelial Cells (HUVEC) were purchased from ALLCELLS. Only early passages of these cell lines were use | Full Paper |
2012 | Silverman, AM;Nakata, R;Shimada, H;Sposto, R;DeClerck, YA; | A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma | Cancer research | 22389450 | Interleukin-6 (IL-6) is a pleiotropic cytokine with a broad range of physiologic and pathologic functions. Because in cancer, IL-6 contributes to a microenvironment that promotes tumor Save | Full Paper |
2012 | Pacienza, N;Yoshimitsu, M;Mizue, N;Au, BC;Wang, JC;Fan, X;Takenaka, T;Medin, JA; | Lentivector transduction improves outcomes over transplantation of human HSCs alone in NOD/SCID/Fabry mice | Molecular therapy : the journal of the American Society of Gene Therapy | 22472949 | Human mobilized PB CD34+ cells were obtained from AllCells (Emeryville, CA). These cells were rapidly thawed following the manufacturer's recommendations. After thawing, ~85% of the cells were viable as assessed by trypan blue exclusion, while the hC | Full Paper |
2012 | Lee, EJ;Choi, EK;Kang, SK;Kim, GH;Park, JY;Kang, HJ;Lee, SW;Kim, KH;Kwon, JS;Lee, KH;Ahn, Y;Lee, HJ;Cho, HJ;Choi, SJ;Oh, WI;Park, YB;Kim, HS; | N-cadherin determines individual variations in the therapeutic efficacy of human umbilical cord blood-derived mesenchymal stem cells in a rat model of myocardial infarction | Molecular therapy : the journal of the American Society of Gene Therapy | 22068423 | PBMCs (1 × 105/well) or hUCB-MSCs (1 × 102, 1 × 103 or 1 × 104/well) in 96-well tissue culture plates. The PBMCs were purchased from AllCells (Emeryville, CA) The stimulator PBMCs and hUCB-MSCs were inactivated by treatment with 10 µg/ml mitomycin-C | Full Paper |
2012 | Goh, KC;Novotny-Diermayr, V;Hart, S;Ong, LC;Loh, YK;Cheong, A;Tan, YC;Hu, C;Jayaraman, R;William, AD;Sun, ET;Dymock, BW;Ong, KH;Ethirajulu, K;Burrows, F;Wood, JM; | TG02, a novel oral multi-kinase inhibitor of CDKs, JAK2 and FLT3 with potent anti-leukemic properties | Leukemia | 21860433 | Primary cells—either peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients—were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex ( Save | Full Paper |
2012 | Boitano, AE;de Lichtervelde, L;Snead, JL;Cooke, MP;Schultz, PG; | An image-based screen identifies a small molecule regulator of megakaryopoiesis | Proceedings of the National Academy of Sciences of the United States of America | 22891346 | Human CD34+ cells were purified from fresh human leukophoresed-mobilized peripheral blood (AllCells) or from umbilical-cord blood (Bioreclamation), as noted, using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following manufacturer's | Full Paper |
2012 | Kobune, M;Iyama, S;Kikuchi, S;Horiguchi, H;Sato, T;Murase, K;Kawano, Y;Takada, K;Ono, K;Kamihara, Y;Hayashi, T;Miyanishi, K;Sato, Y;Takimoto, R;Kato, J; | Stromal cells expressing hedgehog-interacting protein regulate the proliferation of myeloid neoplasms | Blood cancer journal | 22961059 | Minneapolis, MN, USA). 5-Aza-2′-deoxycytidine (5-aza-dC) was obtained from Sigma (St Louis, MO, USA). Total RNA derived from mobilized peripheral blood (PB), BM or CB CD34+ cells was purchased from AllCells, LLC (Toronto, Ontario, Canada). Human myel | Full Paper |
2012 | Novotny-Diermayr, V;Hart, S;Goh, KC;Cheong, A;Ong, LC;Hentze, H;Pasha, MK;Jayaraman, R;Ethirajulu, K;Wood, JM; | The oral HDAC inhibitor pracinostat (SB939) is efficacious and synergistic with the JAK2 inhibitor pacritinib (SB1518) in preclinical models of AML | Blood cancer journal | 22829971 | Peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex (Culver City, CA, USA) Save | Full Paper |
2012 | Gao, N;Cheng, S;Budhraja, A;Gao, Z;Chen, J;Liu, EH;Huang, C;Chen, D;Yang, Z;Liu, Q;Li, P;Shi, X;Zhang, Z; | Ursolic acid induces apoptosis in human leukaemia cells and exhibits anti-leukaemic activity in nude mice through the PKB pathway | British journal of pharmacology | 21950524 | Blasts were suspended at 8 × 105 mL-1 and incubated in RPMI 1640 medium containing 10% FBS in 24-well plates. Fresh normal bone marrow mononuclear cells were purchased from Allcells (Emeryvill, CA). After being washed and counted, cells were suspende | Full Paper |
2012 | De Giovanni, C;Nicoletti, G;Landuzzi, L;Romani, F;Croci, S;Palladini, A;Murgo, A;Antognoli, A;Ianzano, ML;Stivani, V;Grosso, V;Iezzi, M;Stramucci, L;Barbieri, E;Lemoli, RM;Nanni, P;Lollini, PL; | Human responses against HER-2-positive cancer cells in human immune system-engrafted mice | British journal of cancer | 22929887 | ec, Bergisch Gladbach, Germany) to obtain highly purified cells (mean purity 93%±6%) as previously described (Rossi et al, 2007). For challenge experiments, CD34+ or CD133+ cells were purchased from AllCells (Emeryville, CA, USA). All the experiments | Full Paper |
2012 | Fukushima, H;Kawanabe, N;Murata, S;Ishihara, Y;Yanagita, T;Balam, TA;Yamashiro, T; | SSEA-4 is a marker of human deciduous periodontal ligament stem cells | Journal of dental research | 22895512 | Human bone marrow cells were purchased from AllCells (Emeryville, CA, USA) and were maintained in low-glucose DMEM supplemented with 10% HIFBS and penicillin/ streptomycin. Save | Full Paper |
2012 | Kim, J;Denu, RA;Dollar, BA;Escalante, LE;Kuether, JP;Callander, NS;Asimakopoulos, F;Hematti, P; | Macrophages and mesenchymal stromal cells support survival and proliferation of multiple myeloma cells | British journal of haematology | 22583117 | Bone marrow (BM) microenvironment provides crucial supports to normal and malignant hematopoiesis and several types of cells such as endothelial cells, osteoblasts and Save | Full Paper |
2012 | Cokic, VP;Bhattacharya, B;Beleslin-Cokic, BB;Noguchi, CT;Puri, RK;Schechter, AN; | JAK-STAT and AKT pathway-coupled genes in erythroid progenitor cells through ontogeny | Journal of translational medicine | 22676255 | mmunomagnetic selection with MACS cell isolation system (Miltenyi Biotec, Auburn, CA). Commercial FL-derived CD34+ cells (Cambrex Bio Science, Inc., Walkersville, MD), CB- and BM-derived CD34+ cells (AllCells LLC, Berkeley, CA) are also isolated by p | Full Paper |
2012 | Mao, M;Sudhahar, V;Ansenberger-Fricano, K;Fernandes, DC;Tanaka, LY;Fukai, T;Laurindo, FR;Mason, RP;Vasquez-Vivar, J;Minshall, RD;Stadler, K;Bonini, MG; | Nitroglycerin drives endothelial nitric oxide synthase activation via the phosphatidylinositol 3-kinase/protein kinase B pathway | Free radical biology & medicine | 22037515 | Human and bovine aortic endothelial cells (HAEC or BAEC; Allcells, Emeryville, CA, USA), human microvascular endothelial cells (HMEC), and primary mouse endothelial cells (MEC) were cultured at 37 °C, 5% CO2 in corresponding endothelial medium with s | Full Paper |
2012 | William, AD;Lee, AC;Poulsen, A;Goh, KC;Madan, B;Hart, S;Tan, E;Wang, H;Nagaraj, H;Chen, D;Lee, CP;Sun, ET;Jayaraman, R;Pasha, MK;Ethirajulu, K;Wood, JM;Dymock, BW; | Discovery of the macrocycle (9E)-15-(2-(pyrrolidin-1-yl)ethoxy)-7,12,25-trioxa-19,21,24-triaza-tetracyclo[18.3.1.1(2,5).1(14,18)]hexacosa-1(24),2,4,9,14(26),15,17,20,22-nonaene (SB1578), a potent inhibitor of janus kinase 2/fms-like tyrosine kinase-3 (JAK2/FLT3) for the treatment of rheumatoid arthritis | Journal of medicinal chemistry | 22339472 | These cells were cultured as described previously. (34) Human CD4+ T cells, isolated from peripheral blood mononuclear cells with 95% purity, were purchased from AllCells (Emeryville, CA) and maintained in X-VIVO 20 media (Lonza, Basel, Switzerland) | Full Paper |
2012 | Tran, CA;Torres-Coronado, M;Gardner, A;Gu, A;Vu, H;Rao, A;Cao, LF;Ahmed, A;Digiusto, D; | Optimized processing of growth factor mobilized peripheral blood CD34+ products by counterflow centrifugal elutriation | Stem cells translational medicine | 23197821 | HPC-A products were obtained from AllCells LLC (Emeryville, CA, http://www.allcells.com), Key Biologics LLC (Memphis, TN, http://www.keybiologics.com), or Progenitor Cell Therapy LLC (Mountain View, CA, http://www.pctcelltherapy.com). HPC-A products | Full Paper |
2012 | Rodriguez-Menocal, L;Salgado, M;Ford, D;Van Badiavas, E; | Stimulation of skin and wound fibroblast migration by mesenchymal stem cells derived from normal donors and chronic wound patients | Stem cells translational medicine | 23197781 | We isolated and expanded human MSCs from fresh whole bone marrow obtained from normal donors and chronic wound patients. Normal donor bone marrow was acquired from AllCells LLC (Emeryville, CA, http://www.allcells.com). Chronic wound patient bone mar | Full Paper |
2012 | Oskouei, BN;Lamirault, G;Joseph, C;Treuer, AV;Landa, S;Da Silva, J;Hatzistergos, K;Dauer, M;Balkan, W;McNiece, I;Hare, JM; | Increased potency of cardiac stem cells compared with bone marrow mesenchymal stem cells in cardiac repair | Stem cells translational medicine | 23197758 | Human bone marrow aspirates were obtained from AllCells LLC (Emeryville, CA, http://allcells.com). Low-density mononuclear cells were separated from human bone marrow aspirates and cultured as described (see supplemental online data). Adherent MSCs w | Full Paper |
2012 | Soler, R;Füllhase, C;Hanson, A;Campeau, L;Santos, C;Andersson, KE; | Stem cell therapy ameliorates bladder dysfunction in an animal model of Parkinson disease | The Journal of urology | 22341818 | Different cell based therapies have been tested, focusing on motor function. We evaluated the effect of human amniotic fluid stem cells and bone marrow derived mesenchymal stem cells (ALLCELLS, Emeryville, California) on bladder dysfunction in a rat | Full Paper |
2012 | Hirata, Y;Ikeda, K;Sudoh, M;Tokunaga, Y;Suzuki, A;Weng, L;Ohta, M;Tobita, Y;Okano, K;Ozeki, K;Kawasaki, K;Tsukuda, T;Katsume, A;Aoki, Y;Umehara, T;Sekiguchi, S;Toyoda, T;Shimotohno, K;Soga, T;Nishijima, M;Taguchi, R;Kohara, M; | Self-enhancement of hepatitis C virus replication by promotion of specific sphingolipid biosynthesis | PLoS pathogens | 22916015 | les containing 200 µg protein were assayed for SPT activity using [14C]-serine and palmitoyl-CoA (Sigma-Aldrich, St. Louis, MO, USA) as substrates. Proliferation assay Human peripheral blood cells (AllCells, Emeryville, CA, USA) were plated onto 96- | Full Paper |
2012 | Köllmer, M;Keskar, V;Hauk, TG;Collins, JM;Russell, B;Gemeinhart, RA; | Stem cell-derived extracellular matrix enables survival and multilineage differentiation within superporous hydrogels | Biomacromolecules | 22404228 | Human bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque PLUS solution and RosetteSep Save | Full Paper |
2012 | Sharma, SM;Fuchsluger, T;Ahmad, S;Katikireddy, KR;Armant, M;Dana, R;Jurkunas, UV; | Comparative analysis of human-derived feeder layers with 3T3 fibroblasts for the ex vivo expansion of human limbal and oral epithelium | Stem cell reviews and reports | 21964568 | Bone marrow samples (3-10 ml) were purchased from AllCells (Emeryville, CA) and plated in T175 flasks in growth media consisting of α-MEM (Lonza, Walkersville, MD) supplemented Save | Full Paper |
2012 | McNamara, LA;Ganesh, JA;Collins, KL; | Latent HIV-1 infection occurs in multiple subsets of hematopoietic progenitor cells and is reversed by NF-κB activation | Journal of virology | 22718820 | bone marrow (BM) was obtained commercially (AllCells Ltd.); mononuclear cells were purified Presorted CD133 + BM or CB cells were obtained commercially (AllCells Ltd.) and were Save | Full Paper |
2012 | Yu, X;McCarthy, PJ;Wang, Z;Gorlen, DA;Mertz, JE; | Shutoff of BZLF1 gene expression is necessary for immortalization of primary B cells by Epstein-Barr virus | Journal of virology | 22623769 | B-cell proliferation assay. Human primary blood B cells were purchased from AllCells (Emeryville, CA), infected with the indicated amount of virus in green Raji units (GRU)/cell, aliquoted at 1 × 104 cells per well in 96-well plates, incubated at 37° | Full Paper |
2012 | Nan, XW;Tang, GJ;Xu, JG;Peng, TS;Miao, LS;Xu, ZG;Bai, C;Yu, P;Gao, YL;Hao, BT; | Imaging manifestations of renal primary neuroectodermal tumors | Chinese medical journal | 23044335 | Pathologicaland jmm unohistochemicalfindingsshowed portionofthetum ornecrosedanduni—morp hologicaloval orround sm allcells with rosettes structure underlight mi croscope.Im Save | Full Paper |
2012 | Lin, W;Oh, SK;Choo, AB;George, AJ; | Activated T cells modulate immunosuppression by embryonic-and bone marrow-derived mesenchymal stromal cells through a feedback mechanism | Cytotherapy | 22136295 | Frozen peripheral blood mononuclear cells (PBMC) were purchased from Allcells (Emeryville, CA, USA). All isolated T-cell cultures were maintained in Roswell Park Memorial Institute (RPMI)-1640 supplemented with 10% FBS, 2 mm l-glutamine, 50 U/mL peni | Full Paper |
2012 | Akalin, A;Garrett-Bakelman, FE;Kormaksson, M;Busuttil, J;Zhang, L;Khrebtukova, I;Milne, TA;Huang, Y;Biswas, D;Hess, JL;Allis, CD;Roeder, RG;Valk, PJ;Löwenberg, B;Delwel, R;Fernandez, HF;Paietta, E;Tallman, MS;Schroth, GP;Mason, CE;Melnick, A;Figueroa, ME; | Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia | PLoS genetics | 22737091 | kemia gene rearranged AML samples harboring t(9;11) (MLL1 and MLL2) and one additional AML sample (AML) were available for processing. Two normal CD34+ bone marrow control samples were purchased from AllCells, LLC (Emeryville, CA, USA). Institutional | Full Paper |
2012 | Chaudhry, P;Yang, X;Wagner, M;Jong, A;Wu, L; | Retinoid-regulated FGF8f secretion by osteoblasts bypasses retinoid stimuli to mediate granulocytic differentiation of myeloid leukemia cells | Molecular cancer therapeutics | 22135230 | Normal human primitive hematopoietic CD34 + cells were obtained from AllCells and CD34 cells, certified to be HIV- and Mycoplasma-free by AllCells, were cultured for maxi Save | Full Paper |
2012 | Budhraja, A;Gao, N;Zhang, Z;Son, YO;Cheng, S;Wang, X;Ding, S;Hitron, A;Chen, G;Luo, J;Shi, X; | Apigenin induces apoptosis in human leukemia cells and exhibits anti-leukemic activity in vivo | Molecular cancer therapeutics | 22084167 | U937, Jurkat, and HL60 human leukemia cells were obtained from American Type Culture Collection and grown in RPMI-1640 medium with 10% FBS. Normal peripheral blood mononuclear cells (NPBMNC) were obtained from AllCells and maintained in RPMI-1640 wit | Full Paper |
2012 | de Lichtervelde, L;Antal, CE;Boitano, AE;Wang, Y;Krastel, P;Petersen, F;Newton, AC;Cooke, MP;Schultz, PG; | Euphohelioscopin A is a PKC activator capable of inducing macrophage differentiation | Chemistry & biology | 22921066 | R & D Systems]) unless otherwise indicated. Human mPB CD34+ cells were purified from fresh human leukophoresed mobilized peripheral blood (AllCells) using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following manufacturer’s protocols | Full Paper |
2012 | Qu, X;Zhuang, G;Yu, L;Meng, G;Ferrara, N; | Induction of Bv8 expression by granulocyte colony-stimulating factor in CD11b+Gr1+ cells: key role of Stat3 signaling | The Journal of biological chemistry | 22528488 | Freshly isolated bone marrow cells from five different healthy donors were obtained from AllCells (Emeryville, CA). Red blood cells were lysed by adding 4 volumes of 1× buffered Save | Full Paper |
2012 | Rowe, DD;Leonardo, CC;Recio, JA;Collier, LA;Willing, AE;Pennypacker, KR; | Human umbilical cord blood cells protect oligodendrocytes from brain ischemia through Akt signal transduction | The Journal of biological chemistry | 22158864 | Human umbilical cord blood (HUCB) cells protect the brain against ischemic injury, yet the mechanism of protection remains unclear. Using both in vitro and in vivo paradigms, this Save | Full Paper |
2012 | Madan, B;Goh, KC;Hart, S;William, AD;Jayaraman, R;Ethirajulu, K;Dymock, BW;Wood, JM; | SB1578, a novel inhibitor of JAK2, FLT3, and c-Fms for the treatment of rheumatoid arthritis | Journal of immunology (Baltimore, Md. : 1950) | 22962687 | Cells CD4+ T cells isolated from human PBMCs, with 95% purity were obtained from AllCells (Emeryville, CA). The THP-1 cell line was purchased from American Type Culture Collection and maintained as recommended | Full Paper |
2012 | Liu, W;Yan, M;Liu, Y;McLeish, KR;Coleman, WG;Rodgers, GP; | Olfactomedin 4 inhibits cathepsin C-mediated protease activities, thereby modulating neutrophil killing of Staphylococcus aureus and Escherichia coli in mice | Journal of immunology (Baltimore, Md. : 1950) | 22844115 | Human fresh bone-marrow neutrophils were obtained from AllCells (Emeryville, CA). Mouse neutrophils were isolated from bone marrow. The femur was removed from mice, then Save | Full Paper |
2012 | Ohtsubo, S;Ishikawa, M;Kamei, N;Kijima, Y;Suzuki, O;Sunagawa, T;Higashi, Y;Masuda, H;Asahara, T;Ochi, M; | The therapeutic potential of ex vivo expanded CD133+ cells derived from human peripheral blood for peripheral nerve injuries | Journal of neurosurgery | 22880720 | We used granulocyte-colony stimulating factor-mobilized CD133+ cells derived from human peripheral blood (lot no. PCA1521B, AllCells) for these experiments. The fresh CD133+ cells were cultured with serum-free medium (StemSpan, Stem Cell Technologies | Full Paper |
2012 | Kadam, SK;Farmen, M;Brandt, JT; | Quantitative measurement of cell-free plasma DNA and applications for detecting tumor genetic variation and promoter methylation in a clinical setting | The Journal of molecular diagnostics : JMD | 22579630 | Commercially available pooled human plasma (AllCells, Emeryville, CA) was used to determine matrix effect and for DNA spike-in experiments. All blood samples from healthy controls Save | Full Paper |
2012 | Magga, J;Savchenko, E;Malm, T;Rolova, T;Pollari, E;Valonen, P;Lehtonen, Š;Jantunen, E;Aarnio, J;Lehenkari, P;Koistinaho, M;Muona, A;Koistinaho, J; | Production of monocytic cells from bone marrow stem cells: therapeutic usage in Alzheimer's disease | Journal of cellular and molecular medicine | 21777378 | Flt-3, 20 ng/ml IL-6, 20 ng/ml IL-3, in humidified atmosphere at 37°C in 5% CO2. Cells were cultivated and differentiated as described earlier. Human PB GCSF-mobilized CD34 cells were obtained from AllCells and cultivated similarly to BM-originated c | Full Paper |
2012 | Xia, Y;Wübbeler, JH;Qi, Q;Steinbüchel, A; | Employing a recombinant strain of Advenella mimigardefordensis for biotechnical production of Homopolythioesters from 3,3'-dithiodipropionic acid | Applied and environmental microbiology | 22344658 | AftertheirODhadreached500Klettunits,allcells were transferred into the same MS medium containing 7 g/liter glycerol plus 9 g/liter DTDP as a precursor. (A) Increase of OD (Klett unit) Save | Full Paper |
2012 | Pei, L;Choi, JH;Liu, J;Lee, EJ;McCarthy, B;Wilson, JM;Speir, E;Awan, F;Tae, H;Arthur, G;Schnabel, JL;Taylor, KH;Wang, X;Xu, D;Ding, HF;Munn, DH;Caldwell, C;Shi, H; | Genome-wide DNA methylation analysis reveals novel epigenetic changes in chronic lymphocytic leukemia | Epigenetics | 22534504 | CD19+/CD27+ memory B-cell, and CD19+/CD27- naïve B-cell samples used in the RRBS analysis were purchased from ALLCELLs Inc. Other normal CD19+ B-cell samples were purified from leuko-enriched blood samples purchased from a local blood bank. Mononucle | Full Paper |
2012 | Behbehani, GK;Bendall, SC;Clutter, MR;Fantl, WJ;Nolan, GP; | Single-cell mass cytometry adapted to measurements of the cell cycle | Cytometry. Part A : the journal of the International Society for Analytical Cytology | 22693166 | Mass cytometry is a recently introduced technology that utilizes transition element isotope‐tagged antibodies for protein detection on a single‐cell basis. By circumventing the limitations Save | Full Paper |
2012 | Kanagal-Shamanna, R;Zhao, W;Vadhan-Raj, S;Nguyen, MH;Fernandez, MH;Medeiros, LJ;Bueso-Ramos, CE; | Over-expression of CYP2E1 mRNA and protein: implications of xenobiotic induced damage in patients with de novo acute myeloid leukemia with inv(16)(p13.1q22); CBFβ-MYH11 | International journal of environmental research and public health | 23066397 | Island, NY, USA). cDNA synthesis was performed using the QuantiTect RT Kit (Qiagen, Valencia, CA, USA) according to manufacturer’s instructions [14]. The commercially produced normal BM CD34 cDNA (AllCells, LLC, Emeryville, CA, USA) was also used in | Full Paper |
2012 | Puri, N;Sodhi, K;Haarstad, M;Kim, DH;Bohinc, S;Foglio, E;Favero, G;Abraham, NG; | Heme induced oxidative stress attenuates sirtuin1 and enhances adipogenesis in mesenchymal stem cells and mouse pre-adipocytes | Journal of cellular biochemistry | 22234917 | For human MSCs, frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential Save | Full Paper |
2012 | Spencer, R; | Magnetic resonance in tissue engineering | NMR in biomedicine | 22134896 | Mesenchymal stem cells isolated from fresh adult human bone marrow were obtained commercially by AllCells (AllCells LLC, Berkeley, CA, USA). Nucleated cells were incubated in a Save | Full Paper |
2012 | Guo, LW;Wu, Q;Green, B;Nolen, G;Shi, L;Losurdo, J;Deng, H;Bauer, S;Fang, JL;Ning, B; | Cytotoxicity and inhibitory effects of low-concentration triclosan on adipogenic differentiation of human mesenchymal stem cells | Toxicology and applied pharmacology | 22726953 | Human adult mesenchymal stem cells (hMSCs) were originally provided by AllCells (Emeryville, CA). hMSCs were grown in alpha Minimum Essential Medium (αMEM), supplemented Save | Full Paper |
2012 | Xu, D;Kim, Y;Postelnek, J;Vu, MD;Hu, DQ;Liao, C;Bradshaw, M;Hsu, J;Zhang, J;Pashine, A;Srinivasan, D;Woods, J;Levin, A;O'Mahony, A;Owens, TD;Lou, Y;Hill, RJ;Narula, S;DeMartino, J;Fine, JS; | RN486, a selective Bruton's tyrosine kinase inhibitor, abrogates immune hypersensitivity responses and arthritis in rodents | The Journal of pharmacology and experimental therapeutics | 22228807 | One million human cord blood-derived CD34+ hematopoietic stem cells (AllCells, LLC, Emeryville, CA) were cultured in a serum-free StemPro-34 complete medium (Invitrogen) with supplements, stem cell factor (100 ng/ml), and IL-6 (50 ng/ml). IL-3 (10 ng | Full Paper |
2012 | Pirlo, RK;Wu, P;Liu, J;Ringeisen, B; | PLGA/hydrogel biopapers as a stackable substrate for printing HUVEC networks via BioLP | Biotechnology and bioengineering | 21830203 | Two major challenges in tissue engineering are mimicking the native cell-cell arrangements of tissues and maintaining viability of three‐dimension (3D) tissues thicker than 300 µm. Save | Full Paper |
2012 | Zou, C;Chou, BK;Dowey, SN;Tsang, K;Huang, X;Liu, CF;Smith, C;Yen, J;Mali, P;Zhang, YA;Cheng, L;Ye, Z; | Efficient derivation and genetic modifications of human pluripotent stem cells on engineered human feeder cell lines | Stem cells and development | 22225458 | Bone marrow-derived MSCs were established from CD34-depleted mononuclear cells (MNCs) (AllCells) as previously described [16], from adult donors (such as a male donor #BM2426). Primary MSCs and immortalized MSCs were cultured in Dulbecco's modified E | Full Paper |
2012 | Aslanidi, GV;Rivers, AE;Ortiz, L;Govindasamy, L;Ling, C;Jayandharan, GR;Zolotukhin, S;Agbandje-McKenna, M;Srivastava, A; | High-efficiency transduction of human monocyte-derived dendritic cells by capsid-modified recombinant AAV2 vectors | Vaccine | 22497875 | DMEM (Invitrogen) supplemented with 10% FBS (Sigma) and antibiotics (Lonza). Leukapheresis-derived peripheral blood mononuclear cells (PBMCs) (AllCells) were purified on Ficoll-Paque (GEHeathCare), resuspended in serum-free AIM-V medium (Lonza), and | Full Paper |
2012 | Koestler, DC;Marsit, CJ;Christensen, BC;Accomando, W;Langevin, SM;Houseman, EA;Nelson, HH;Karagas, MR;Wiencke, JK;Kelsey, KT; | Peripheral blood immune cell methylation profiles are associated with nonhematopoietic cancers | Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology | 22714737 | Sorted, normal, human, peripheral blood leukocyte subtypes were purchased from AllCells. Leukocytes were isolated from different, anonymous, nondiseased individuals' whole blood ( Save | Full Paper |
2012 | Hong, L;Wei, N;Joshi, V;Yu, Y;Kim, N;Krishnamachari, Y;Zhang, Q;Salem, AK; | Effects of glucocorticoid receptor small interfering RNA delivered using poly lactic-co-glycolic acid microparticles on proliferation and differentiation capabilities of human mesenchymal stromal cells | Tissue engineering. Part A | 21988716 | All other chemicals and solvents used, including dichloromethane (DCM) and acetonitrile, were of high-performance liquid chromatography analytical grade. Fresh human bone marrow was purchased from Allcells (Emeryville, CA). Invitrogen (Carlsbad, CA) | Full Paper |
2012 | Noto, PB;Bukhtiyarov, Y;Shi, M;McKeever, BM;McGeehan, GM;Lala, DS; | Regulation of sphingomyelin phosphodiesterase acid-like 3A gene (SMPDL3A) by liver X receptors | Molecular pharmacology | 22810003 | Whole blood from human donors was purchased from AllCells, LLC (Emeryville, CA). Human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood collected Save | Full Paper |
2012 | Abdolhosseini, M;Nandula, SR;Song, J;Hirt, H;Gorr, SU; | Lysine substitutions convert a bacterial-agglutinating peptide into a bactericidal peptide that retains anti-lipopolysaccharide activity and low hemolytic activity | Peptides | 22484285 | GL13NH2 is a bacteria-agglutinating peptide derived from the sequence of the salivary protein parotid secretory protein (PSP, BPIFA2, SPLUNC2, C20orf70). The peptide agglutinates Save | Full Paper |
2012 | Berezhnyy, IV;Berezhna, SY; | Fast multi-spectral imaging technique for detection of circulating endothelial cells in human blood samples | Journal of biomedical optics | 23224165 | Blood samples from healthy donors were purchased from AllCells, LLC., Emeryville, CA. The blood was collected via venipuncture in EDTA tubes, stored and delivered at 4°C. Before processing, a white blood cell count was performed using Scepter™ 2.0 ce | Full Paper |
2012 | Shah, SN;Kerr, C;Cope, L;Zambidis, E;Liu, C;Hillion, J;Belton, A;Huso, DL;Resar, LM; | HMGA1 reprograms somatic cells into pluripotent stem cells by inducing stem cell transcriptional networks | PloS one | 23166588 | tocol# MO11M279). All mice were housed in a sterile environment where they had free access to food and water as outlined in our institutional guidelines. Cell Culture MSC1640 mesenchymal stem cells (AllCells, LLC) were maintained in DMEM (low glucos | Full Paper |
2012 | Ringpis, GE;Shimizu, S;Arokium, H;Camba-Colón, J;Carroll, MV;Cortado, R;Xie, Y;Kim, PY;Sahakyan, A;Lowe, EL;Narukawa, M;Kandarian, FN;Burke, BP;Symonds, GP;An, DS;Chen, IS;Kamata, M; | Engineering HIV-1-resistant T-cells from short-hairpin RNA-expressing hematopoietic stem/progenitor cells in humanized BLT mice | PloS one | 23300932 | for 2 hours in the presence of 8 μg/ml of polybrene. Transductions of PBMCs were performed as previously described [23] with indicated cell numbers and MOIs. mPB-CD34+ cells (9.8×104), provided by AllCells (Emeryville, CA), were pre-stimulated with S | Full Paper |
2012 | Zhu, J;Siclari, VA;Liu, F;Spatz, JM;Chandra, A;Divieti Pajevic, P;Qin, L; | Amphiregulin-EGFR signaling mediates the migration of bone marrow mesenchymal progenitors toward PTH-stimulated osteoblasts and osteocytes | PloS one | 23300521 | Human bone marrow aspirates were purchased from Allcells and purified through Ficoll gradient (GE Healthcare) to obtain bone marrow mononuclear cells. These cells were seeded Save | Full Paper |
2012 | Wen, H;Li, Y;Malek, SN;Kim, YC;Xu, J;Chen, P;Xiao, F;Huang, X;Zhou, X;Xuan, Z;Mankala, S;Hou, G;Rowley, JD;Zhang, MQ;Wang, SM; | New fusion transcripts identified in normal karyotype acute myeloid leukemia | PloS one | 23251452 | ry samples with abnormal karyotype AML and eight AML primary samples without karyotype information [Figure 1], of which 38 were from University of Michigan Comprehensive Cancer Center and 7 were from Allcells Inc (AllCells, CA). The use of samples fr | Full Paper |
2012 | Kobayashi, DT;Decker, D;Zaworski, P;Klott, K;McGonigal, J;Ghazal, N;Sly, L;Chung, B;Vanderlugt, J;Chen, KS; | Evaluation of peripheral blood mononuclear cell processing and analysis for Survival Motor Neuron protein | PloS one | 23226377 | A: SMN signal was evaluated in lysates created from a single sample purchased from AllCells. PBMCs were processed in ER4 at densities of 10 6 , 10 7 and 5×10 7 cells/mL and the Save | Full Paper |
2012 | Zhang, LJ;Tao, BB;Wang, MJ;Jin, HM;Zhu, YC; | PI3K p110α isoform-dependent Rho GTPase Rac1 activation mediates H2S-promoted endothelial cell migration via actin cytoskeleton reorganization | PloS one | 22970259 | uman recombinant Rac1 and G-LISA Rac1 activation assay kits were obtained from Cytoskeleton (Denver, CO, USA). Cell culture-Primary human umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Emeryville, CA, USA). Each batch of HUVE | Full Paper |
2012 | Venet, S;Ravn, U;Buatois, V;Gueneau, F;Calloud, S;Kosco-Vilbois, M;Fischer, N; | Transferring the characteristics of naturally occurring and biased antibody repertoires to human antibody libraries by trapping CDRH3 sequences | PloS one | 22937053 | Human CDRH3 from three donors were amplified by nested PCR from commercially available peripheral blood purified cells cDNA (AllCells and BioChain). The first PCR allowed the Save | Full Paper |
2012 | Park, TS;Huo, JS;Peters, A;Talbot, CC;Verma, K;Zimmerlin, L;Kaplan, IM;Zambidis, ET; | Growth factor-activated stem cell circuits and stromal signals cooperatively accelerate non-integrated iPSC reprogramming of human myeloid progenitors | PloS one | 22905176 | For these experiments, highly purified (>96%) CD34 + CD45 + CB cells were received within 24 hours of neonatal harvest from AllCells (catalog number: CB005). On the same day (day Save | Full Paper |
2012 | Milanesi, A;Lee, JW;Li, Z;Da Sacco, S;Villani, V;Cervantes, V;Perin, L;Yu, JS; | β-Cell regeneration mediated by human bone marrow mesenchymal stem cells | PloS one | 22879915 | BMSCs from a normal adult donor were purchased from a commercial source AllCells, LLC (5858 Horton Street, Suite 360, Emeryville, California, 94608 USA; catalog number ABM005) Save | Full Paper |
2012 | Boomsma, RA;Geenen, DL; | Mesenchymal stem cells secrete multiple cytokines that promote angiogenesis and have contrasting effects on chemotaxis and apoptosis | PloS one | 22558198 | TCTCCTC GTCTGCCTCTTTTGGTCAGG Angiogenesis Assay The effect of paracrine factors secreted by MSC into CM on angiogenesis was studied using canine jugular vein vascular endothelial cells (CVEC; AllCells) and the Fibrin Gel In Vitro Angiogenesis | Full Paper |
2012 | Basile, LA;Ellefson, D;Gluzman-Poltorak, Z;Junes-Gill, K;Mar, V;Mendonca, S;Miller, JD;Tom, J;Trinh, A;Gallaher, TK; | HemaMax™, a recombinant human interleukin-12, is a potent mitigator of acute radiation injury in mice and non-human primates | PloS one | 22383962 | clear cells (PBMC) in vitro and the PK and PD characteristics of HemaMax in vivo. Isolation of CD14- PBMC and Quantification of IFN-γ Secretion Human PBMC collected by apheresis were purchased from AllCells (Emeryville, CA, USA). Mouse and rhesus mo | Full Paper |
2012 | Gao, N;Cheng, S;Budhraja, A;Liu, EH;Chen, J;Chen, D;Yang, Z;Luo, J;Shi, X;Zhang, Z; | 3,3'-Diindolylmethane exhibits antileukemic activity in vitro and in vivo through a Akt-dependent process | PloS one | 22363731 | lls were washed and assayed for total number and viability using trypan blue exclusion. Cells were suspended at 8×105/mL for treatment. Fresh normal bone marrow mononuclear cells were purchased from Allcells (Emeryvill, CA). After washing and enumera | Full Paper |
2012 | Rellick, SL;O'Leary, H;Piktel, D;Walton, C;Fortney, JE;Akers, SM;Martin, KH;Denvir, J;Boskovic, G;Primerano, DA;Vos, J;Bailey, N;Gencheva, M;Gibson, LF; | Bone marrow osteoblast damage by chemotherapeutic agents | PloS one | 22363485 | Mediatech), 0.05 µM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), non-essential amino acids and B-FGF solution. H9 cells were moved to HOB feeder layers where indicated. CD34+ bone marrow cells (ALLCELLS, Emeryville, CA) were grown in RPMI-1640 s | Full Paper |
2012 | Houseman, EA;Accomando, WP;Koestler, DC;Christensen, BC;Marsit, CJ;Nelson, HH;Wiencke, JK;Kelsey, KT; | DNA methylation arrays as surrogate measures of cell mixture distribution | BMC bioinformatics | 22568884 | ples, de-identified specimens that were not subject to human subjects review by an institutional review board (IRB). The sorted, normal, human, peripheral blood leukocyte subtypes were purchased from AllCellsⓇ, LLC (Emeryville, CA) and were isolated | Full Paper |
2012 | Christopherson, KW;Frank, RR;Jagan, S;Paganessi, LA;Gregory, SA;Fung, HC; | CD26 protease inhibition improves functional response of unfractionated cord blood, bone marrow, and mobilized peripheral blood cells to CXCL12/SDF-1 | Experimental hematology | 22846168 | Medical Center (RUMC) with institutional review board (IRB) approval. Human G-CSF mobilized peripheral blood (mobPB) was purchased from AllCells, LLC (Emeryville, CA, USA). Mononuclear cells (MNC) were isolated by density gradient centrifugation over | Full Paper |
2012 | Iancu-Rubin, C;Gajzer, D;Mosoyan, G;Feller, F;Mascarenhas, J;Hoffman, R; | Panobinostat (LBH589)-induced acetylation of tubulin impairs megakaryocyte maturation and platelet formation | Experimental hematology | 22381681 | Human MK were generated in liquid cultures from bone marrow (BM)-derived CD34+ cells (AllCells, Inc. Emeryville, CA). Cells were suspended in IMDM medium (Invitrogen, Grand Island, NY) supplemented with 1% penicillin/streptomycin, 1% L-Glutamine (Inv | Full Paper |
2012 | Fujioka, Y;Tanaka, N;Nakanishi, K;Kamei, N;Nakamae, T;Izumi, B;Ohta, R;Ochi, M; | Magnetic field-based delivery of human CD133⁺ cells promotes functional recovery after rat spinal cord injury | Spine | 22246536 | Phosphate-buffered saline (PBS) solution (50 μL) containing 1 × 10 5 of human peripheral blood-derived CD133 + cells (AllCells, Emeryville, CA) was injected into the subarachnoid Save | Full Paper |
2012 | Huovinen, P;Petersen, H; | Particlization in hybrid models | The European Physical Journal A | The first one that we call "allcells" sampling, contains a loop over all hypersurface elements The straightforward "allcells" sampling does not do it, and therefore we have developed the Save | Full Paper | |
2012 | Kusao, I;Shiramizu, B;Liang, CY;Grove, J;Agsalda, M;Troelstrup, D;Velasco, VN;Marshall, A;Whitenack, N;Shikuma, C;Valcour, V; | Cognitive performance related to HIV-1-infected monocytes | The Journal of neuropsychiatry and clinical neurosciences | 22450616 | assay for IL6, IL8, IL10, TNFα, sFAS, sTNFRI, sTNFRII, and MCP1. For the in-vitro experiments, monocytes were separated from commercially-available PBMC (AllCells; Emeryville, CA) using the EasySep Human CD14 Positive Selection Kit (Stem Cell Technol | Full Paper |
2012 | Yu, Y;Wei, N;Stanford, C;Schmidt, T;Hong, L; | In vitro effects of RU486 on proliferation and differentiation capabilities of human bone marrow mesenchymal stromal cells | Steroids | 22093480 | Fresh human bone marrows, donated by healthy donors from 21 to 47 years of age, were purchased from Allcells (Emeryville, CA). Mononuclear cells were isolated using density Save | Full Paper |
2012 | Tatsumi, K;Taatjes, DJ;Wadsworth, MP;Bouchard, BA;Bovill, EG; | Cell adhesion molecule 1 (CADM1) is ubiquitously present in the endothelium and smooth muscle cells of the human macro- and micro-vasculature | Histochemistry and cell biology | 22940845 | Immunostained cells were visualized with an Olympus BX51 fluorescence microscope (Olympus America, Center Valley, PA). For each experiment (n = 3) human umbilical vein ECs (AllCells, Emeryville CA), cultured as per the manufacturer’s directions, were | Full Paper |
2012 | He, M;Chen, P;Arnovitz, S;Li, Y;Huang, H;Neilly, MB;Wei, M;Rowley, JD;Chen, J;Li, Z; | Two isoforms of HOXA9 function differently but work synergistically in human MLL-rearranged leukemia | Blood cells, molecules & diseases | 22633751 | The mononuclear cell (MNC) samples were isolated from normal BM cells purchased from AllCells, LLC. MNC cells were isolated using NycoPrep 1.077A (Axis-Shield, Oslo, Norway) Save;in Dulbecco modified Eagle's medium (DMEM) supplemented with 10% FBS | Full Paper |
2012 | Kobayashi, M;Kawase, T;Horimizu, M;Okuda, K;Wolff, LF;Yoshie, H; | A proposed protocol for the standardized preparation of PRF membranes for clinical use | Biologicals : journal of the International Association of Biological Standardization | 22841724 | Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells, LLC (Emeryville, CA) and maintained in an endothelial cell medium that was fully supplemented with growth factors (HuMedia-EB2; KURABO, Osaka, Japan). For the scratch assay, | Full Paper |
2012 | Aoki, T;Miyazaki, K;Katayama, T;Watanabe, M;Horie, R;Danbara, M;Higashihara, M; | Surface CD3 expression proceeds through both myosin regulatory light chain 9 (MYL9)-dependent and MYL9-independent pathways in Jurkat cells | Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi | 23538510 | K562 cells were transfected using the 96-well Nucleofector Kit SF (Lonza) per manufacturer's recommendations. PBMCs (AllCells) were received on ice, centrifuged at 1,000g for 5 min at room temperature (22 °C) and changed to pre-warmed RPMI medium sup | Full Paper |
2012 | Wakao, S;Kitada, M;Kuroda, Y;Dezawa, M; | Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages | Methods in molecular biology (Clifton, N.J.) | 22167642 | candidate cell source for isolation of Muse cells. We recommend to use adult human dermal fibroblasts (CC-2511, Lonza, Basel, Switzerland; 2320, ScienCell, Carlsbad, CA) and adult human bone marrow stromal cells (PT-2501, Lonza; ABM001, AllCells, Eme | Full Paper |
2012 | Mendelson, A; | Chondrogenesis of Stem/Progenitor Cells by Chemotaxis Using Novel Cell Homing Systems | Thesis | Human bone marrow MSCs were isolated from fresh adult donor samples (AllCells, Emeryville, CA) using our prior methods [94, 96, 115]. Briefly, the bone marrow sample was Save | Full Paper | |
2012 | Balzano, G;Liesch, B; | The role of chroma and scalestep in the recognition of musical intervals in and out of context. | Psychomusicology: A Journal of Research in Music Cognition | The octave presented somewhat of a problem in these analyses; latencies (and errors) tended to be drastically lower in allcells ofthe matrix involving the octave, and because of the Save | Full Paper | |
2012 | Gratien, J; | Implementing Lowest-Order Methods for Diffusive Problems with a DSEL | Modelling and Simulation in Fluid Dynamics in Porous Media | The integration algorithm consists in iterating on the cell elements of the allCells(Th) and evaluating the bilinear expression on each cell. This bilinear expression is composed of: Save | Full Paper | |
2012 | YAN, LI; | Improvements on Humanized NOD/SCID lL-2rg-/-Mice | Thesis | Purified human CD133+ cord blood cells were purchased from AllCells (California). Alternatively, umbilical cord blood was obtained from the Singapore Cord Blood Bank. Red blood Save | Full Paper | |
2012 | Nikdel, A;Jameii, SM;Faez, K; | A novel area coverage protocol based on particle swarm optimization algorithm for wireless sensor networks | Global Journal on Technology | will be provided if the sum of cells which are covered (ie Sumcells set), are equal to the sum of cells which will be covered if all nodes have maximum sensing radius (ie Allcells set). As Save | Full Paper | |
2012 | Huff, RR; | Induction of chondrogenesis from MSCs by BMP7 and TGFB1 in low oxygen tension: expression of superficial zone protein | Thesis | The IRC received the sample as whole bone marrow, purchased commercially (AllCells Inc.). To isolate the MSCs, nucleated cells were recovered from a 25mL sample using a Ficoll- Save | Full Paper | |
2012 | Sunderic, K; | Effects of Mild Heating on the Osteogenic Differentiation of Human Mesenchymal Stem Cells during Inflammation | Thesis | Human bone marrow explanted from the iliac crest of a 24-year old donor was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the Save | Full Paper | |
2012 | Sangokoya, CO; | MicroRNA Function in Cellular Stress Response | Thesis | HbAA CD34+ cells (ALLCells) and HbSS CD34+ cells were purified from blood collected from apheresis with donor consent according to Duke IRB protocol. Mononuclear cells were separated by Ficoll-Hypaque density gradient, and CD34+ cells were enriched b | Full Paper | |
2012 | McNamara, LA; | HIV-1 Infection of Hematopoietic Progenitor Cells. | Thesis | We obtained pre-existing umbilical cord blood lacking subject identifiers after scheduled cesarean section procedures. We obtained fresh whole bone marrow aspirates from a commercial source (AllCells Ltd.). We prepared BMMCs and UCB mononuclear cells | Full Paper | |
2012 | Lowe, RM; | Investigation into the role of novel interferon lambdas in the treatment of viral disease, primarily hepatitis C | Thesis | +L-glutamine and 100ng/mL Macrophage colony-stimulating factor (M-CSF) and incubated for four days to differentiate into macrophages. Mast cells were derived from cord blood mononuclear cells (AllCells), cells were cultured in Iscove’s modified Dulbe | Full Paper | |
2012 | David, G; | Le locus 1q32: susceptibilité aux maladies inflammatoires de l'intestin et rôles biologiques de C1orf106 et KIF21B | Thesis | préparations d’ARN ont été conservées à -80°C. Les ARN des cellules primaires CD4+, CD8+, CD14+ et CD19+ proviennent de la compagnie Allcells (Californie, États-Unis) | Full Paper | |
2012 | Lin, X; | Novel Factors in Myeloproliferative Neoplasms-The Role of NFIB and miRNA Deregulation in Hematopoietic Cell Proliferation and Differentiation | Thesis | JAK2V617F mutation was detected in 7 out of 9 specimens by reverse transcription/PCR (AmpliTaq Gold) and directly sequencing JAK2 sequences of 1849 nucleotides amplified. Normal bone marrow CD34+ cells were purchased from AllCells. To isolate CD34+ c | Full Paper | |
2012 | Budhraja, A; | Molecular mechanism of apigenin induced apoptosis in leukemia | Thesis | Normalperipheral blood mononuclear cells were obtained from AllCells (Emeryville, CA, USA)and were maintained in RPMI 1640 supplemented with 10% FBS. Dr. Ruth Craig( Save | Full Paper | |
2012 | Severson, EBS; | Developing a Novel In-Vitro Neuroinflammation Model for Huntington's Disease | Thesis | Human BM aspirates were purchased from Lonza and AllCells. Density gradient centrifugation (Ficoll-Hypaque) was used to isolate mononuclear BM-MSC from three donors (AC1, Save | Full Paper | |
2012 | Mahmoud, AEM; | Investigating the biochemical activity of some flavonoids, coumarins and modified gold-nanoparticles as anti-breast-cancer agents | Thesis | Allcells were incubated for 2 days at 37 C under 5 % CO2. When the cells reached 50%confluent, the old media was removed and the adherent cells were first washed by IXversene. Save | Full Paper | |
2012 | Suh, SS; | A study of microRNAs associated with multiple myeloma pathogenesis and microRNAs/TP53 feedback circuit in human cancers, multiple myeloma and glioblastoma multiforme | Thesis | with monoclonal mouse anti-human CD138 antibodies using the AutoMACs automated separation system (Miltenyi-Biotec Three of the six healthy PCs were obtained from ALLCELLS, Save | Full Paper | |
2012 | Berk, E; | Dendritic cells regulate the induction of effector and memory CD8+ t cells | Thesis | SW480 and SW620 (ATCC, Manassas, VA) were grown in IMDM+10% FBS, while HUVECs (AllCells, Emeryville, CA) were cultured in HUVEC complete media (Basal media supplemented with HUVEC stimulatory supplement; AllCells). All were washed, reseeded at 20,000 | Full Paper | |
2012 | Peters, HLC; | Characteristics of amyloid precursor-like protein 2 (APLP2) in pancreatic cancer and Ewing's sarcoma | Thesis | Allcells and cellular debris were pelleted by centrifugation at 2,000 xg at 4C for 5 min. Thesupernatant was discarded and pellets containing 5 x 106 cells were resuspended in 100/jL Save | Full Paper | |
2012 | Chung, TY; | Experimental Beam Studies of Plasma-generated Species Interaction with Polymeric Materials and Biomolecules | Thesis | The basic protocol is shown in Figure 3.6. Heparinized fresh human whole blood was acquired from AllCells (Emeryville, CA, USA) and used within 6 h of collection. Samples were prepared by spotting serial diluted lipid A solution on UV/ozone-cleaned s | Full Paper | |
2012 | Hung, KC; | Developing peptide and antibody-mimetic ligands for the cell surface receptor beta2AR and DC-SIGN | Thesis | Sallusto and Lanzavecchia, 1994), except that hPBMCs were from HLA-A2+ patients (AllCells, LLC.). iDCs were treated with M3H4-18-HA, stained with anti-HA antibody, and analyzed by either flow cytometry or microscopy same as 293T cells, except that th | Full Paper | |
2012 | Simonds, EF; | Single-Cell Deep Profiling of Immune Signaling and Drug Responses in Normal and Malignant Human Hematopoiesis | Thesis | obtained from a tissue bank at Princess Margaret Hospital (Toronto, ON), courtesy of Dr. Mark D. Minden. Cryopreserved healthy BMMCs were purchased from AllCells, Inc. (Emeryville, CA). All human samples were obtained with informed consent in complia | Full Paper | |
2012 | Pardee, TS;Gomes, E;Jennings-Gee, J;Caudell, D;Gmeiner, WH; | Unique dual targeting of thymidylate synthase and topoisomerase1 by FdUMP[10] results in high efficacy against AML and low toxicity | Blood | 22362039 | Human cell lines, primary patient samples, or normal human HSCs were thawed and incubated in Stem SpanSFEM media supplemented with Stem Span CC100 cytokine cocktail (StemCell Technologies) for 24 hours with the indicated drug. Cells were then placed | Full Paper |
2012 | Zeiger, AS;Loe, FC;Li, R;Raghunath, M;Van Vliet, KJ; | Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior | PloS one | 22649562 | ganization correlate with an increase in proliferation and a decrease in motility of MSCs. Materials and Methods Cell Culture Human bone-marrow derived mesenchymal stromal or “stem” cells (MSCs; ReachBio Seattle, WA) were expanded until passage 4-6, | Full Paper |
2012 | Jolibois-Quinot, R; | Quantitative correlations among human mesenchymal stem cell mechanical properties and biological function | Thesis | Adult mesenchymal stem cells (referred later as aMSCs) were purchased from commercial sources: ReachBio human bone marrow mesenchymal stem cells - lot number 0090408 (referred later as RB1 - ReachBio LLC, Seattle, WA), and Poietics" human mesenchymal | Full Paper | |
2012 | Maloney, JM; | Chemomechanics of attached and suspended cells | Thesis | I have also been lucky to work with fine coauthors that dependably contributed honest and top-notch data and ideas: Christopher Bruce and Emily Walton (MIT), Franziska Lautenschläger and Jochen Guck (University of Cambridge), Dessy Nikova (MIT), Emer | Full Paper | |
2011 | Xu, B;Roos, JL;Dexheimer, P;Boone, B;Plummer, B;Levy, S;Gogos, JA;Karayiorgou, M; | Exome sequencing supports a de novo mutational paradigm for schizophrenia | Nat. Genet. | 21822266 | BX, JAG and MK designed the study, interpreted the data and prepared the manuscript; BX developed the analysis pipeline and had the primary role in analysis and validation of sequence data; JLR collected the samples and was the primary clinician on t | Full Paper |
2011 | Wood, AM;Van Bortle, K;Ramos, E;Takenaka, N;Rohrbaugh, M;Jones, BC;Jones, KC;Corces, VG; | Regulation of chromatin organization and inducible gene expression by a Drosophila insulator | Mol. Cell | 21981916 | Chromatin immunoprecipitation was performed as described (Bushey et al., 2009). To generate sequencing libraries ChIP DNA was prepared for adaptor ligation by end repair (‘End-It DNA End Repair Kit’ - Epicentre Cat# ER0720) and addition of ‘A’ base t | Full Paper |
2011 | Jänne, PA;Boss, DS;Camidge, DR;Britten, CD;Engelman, JA;Garon, EB;Guo, F;Wong, S;Liang, J;Letrent, S;Millham, R;Taylor, I;Eckhardt, SG;Schellens, JH; | Phase I dose-escalation study of the pan-HER inhibitor, PF299804, in patients with advanced malignant solid tumors | Clin. Cancer Res. | 21220471 | Immunohistochemistry analysis was performed by QualTek (Newtown, PA) and was automated throughout using a TechMate 500 or TechMate 1000 (BioTek Solutions/Ventana Medical Systems). Antibodies used were: pERK 1/2 clone 20G11 (Cell Signaling Cat # 4376) | Full Paper |
2011 | Ahmad, I;Morton, JP;Singh, LB;Radulescu, SM; | β-Catenin activation synergizes with PTEN loss to cause bladder cancer formation | Oncogene | invasive bladder carcinoma. We next looked at human bladder UCC using a tissue microarray of 80 cases, 60 UCC (transitional cell carcinomas) and 20 benign controls (Folio Biosciences, Columbus, OH, USA). Using the histoscore | Full Paper | |
2011 | Teichert, AE;Elalieh, H;Elias, PM;Welsh, J;Bikle, DD; | Overexpression of hedgehog signaling is associated with epidermal tumor formation in vitamin D receptor-null mice | J. Invest. Dermatol. | 21814234 | ffinity-purified, biotinylated goat anti-rabbit IgG, followed by ABC-peroxidase reagent, both purchased from Vector (Burlingame, CA). Peroxidase activity was detected with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followed | Full Paper |
2011 | Kelly, RK;Olson, DL;Sun, Y;Wen, D;Wortham, KA;Antognetti, G;Cheung, AE;Orozco, OE;Yang, L;Bailly, V;Sanicola, M; | An antibody-cytotoxic conjugate, BIIB015, is a new targeted therapy for Cripto positive tumours | Eur. J. Cancer | 21458984 | 2.3. Immunohistochemistry. Human breast, colon, lung and ovarian formalin-fixed paraffin-embedded tumour tissue arrays (Imgenex, San Diego, CA, Folio Biosciences, Columbus, OH and Zymed, San Francisco, CA) were stained as described previously | Full Paper |
2011 | Ahmad, I;Singh, LB;Foth, M;Morris, CA;Taketo, MM;Wu, XR;Leung, HY;Sansom, OJ;Iwata, T; | K-Ras and β-catenin mutations cooperate with Fgfr3 mutations in mice to promote tumorigenesis in the skin and lung, but not in the bladder | Dis Model Mech | 21504907 | Finally, we have examined the ERK-MAPK signaling pathway in human UCC by using a tissue microarray (TMA) that contains 60 UCC and 20 benign controls (Folio Biosciences, OH). Using the Histoscore technique, we have quantified the staining intensities | Full Paper |
2011 | Rokkam, D;Lafemina, MJ;Lee, JW;Matthay, MA;Frank, JA; | Claudin-4 levels are associated with intact alveolar fluid clearance in human lungs | Am. J. Pathol. | 21741940 | controls. Additional normal lung tissue specimens (n = 3) were also obtained (US Biomax, Rockville, MD; Folio Biosciences, Columbus, OH) and immunostained for claudin-4, as previously described. Histology Scoring. The | Full Paper |
2011 | Kent, BN;Salichos, L;Gibbons, JG;Rokas, A;Newton, IL;Clark, ME;Bordenstein, SR; | Complete bacteriophage transfer in a bacterial endosymbiont (Wolbachia) determined by targeted genome capture | Genome Biol Evol | 21292630 | Genomic DNA was extracted from ∼200 abdomens of the B-Wolbachia infected N. vitripennis 4.9 using the QIAGEN DNeasy Kit insect tissue protocol. DNA was treated with 100 mg/ml RNaseA and incubated at room temperature for 2 min. The resulting DNA was q | Full Paper |
2011 | Jiang, Y;Lu, J;Peatman, E;Kucuktas, H;Liu, S;Wang, S;Sun, F;Liu, Z; | A pilot study for channel catfish whole genome sequencing and de novo assembly | BMC Genomics | 22192763 | The culture supernatant was decanted and the block was inverted and tapped gently on paper towels to remove remaining liquid. BAC DNA was isolated using the Perfectprep™ BAC 96 kit (Eppendorf North America, Westbury, NY) according to the manufacturer | Full Paper |
2011 | Pitts, RJ;Rinker, DC;Jones, PL;Rokas, A;Zwiebel, LJ; | Transcriptome profiling of chemosensory appendages in the malaria vector Anopheles gambiae reveals tissue- and sex-specific signatures of odor coding | BMC Genomics | 21619637 | We thank the following individuals: Zhen Li for mosquito rearing; Travis Clark, Chelsea Baker and the Vanderbilt Genomics Shared Resource for sample preparation and Illumina sequencing; Shawn Levy and the Genomic Services Lab at HudsonAlpha Institute | Full Paper |
2011 | Liu, S;Zhou, Z;Lu, J;Sun, F;Wang, S;Liu, H;Jiang, Y;Kucuktas, H;Kaltenboeck, L;Peatman, E;Liu, Z; | Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP array | BMC Genomics | 21255432 | ;... sing the RNeasy plus Mini Kit (Qiagen, Valencia, CA, USA) with DNase I (Invitrogen, USA) treatment following the manufacturer's protocol. Illumina sequencing Sequencing was conducted commercially in HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2011 | Weiss, L;Bernstein, S;Jones, R;Amunugama, R;Krizman, D;Jebailey, L;Almogi-Hazan, O;Hazan, O;Yekhtin, Z;Yachtin, J;Shiner, R;Reibstein, I;Triche, E;Slavin, S;Or, R;Barnea, ER; | Preimplantation factor (PIF) analog prevents type I diabetes mellitus (TIDM) development by preserving pancreatic function in NOD mice | Endocrine | 21424847 | Department of Bone Marrow Transplantation and Cancer Immunotherapy, Hadassah University Hospital Ein Kerem, Hebrew University, Jerusalem, Israel Lola Weiss, Osnat Hazan, Janna Yachtin, Reut Shiner, Israel Reibstein & Reuven Or QualTek Molecular Labor | Full Paper |
2011 | Pawar, H;Kashyap, MK;Sahasrabuddhe, NA;Renuse, S;Harsha, HC;Kumar, P;Sharma, J;Kandasamy, K;Marimuthu, A;Nair, B;Rajagopalan, S;Maharudraiah, J;Premalatha, CS;Kumar, KV;Vijayakumar, M;Chaerkady, R;Prasad, TS;Kumar, RV;Kumar, RV;Pandey, A; | Quantitative tissue proteomics of esophageal squamous cell carcinoma for novel biomarker discovery | Cancer Biol. Ther. | 21743296 | The ESCCs varied from tumor grade I to grade III from patients in the age group of 39-79 y. A second group of tissue microarrays obtained from FolioBio (Columbus, OH) consisted of 40 ESCC tissues with matched adjacent normal esophageal squamous epith | Full Paper |
2011 | Bunting, RA;Duffy, KE;Lamb, RJ;San Mateo, LR;Smalley, K;Raymond, H;Liu, X;Petley, T;Fisher, J;Beck, H;Flavell, RA;Alexopoulou, L;Ward, CK; | Novel antagonist antibody to TLR3 blocks poly(I:C)-induced inflammation in vivo and in vitro | Cell. Immunol. | 21092943 | Mouse tissues (pancreas, inguinal and mesenteric lymph node, brain, liver, heart, trachea, colon, kidneys, lungs, and spleen) from three C57BL/6 and three TLR3KO mice were fixed in 10% neutral buffered formalin and processed for immunohistochemistry | Full Paper |
2011 | Trejos, AL;Jayaraman, S;Patel, RV;Naish, MD;Schlachta, CM; | Force sensing in natural orifice transluminal endoscopic surgery | Surg Endosc | 20559663 | allowing the flexible shaft to bend freely. The shaft and the wires were covered with heat-shrink thin-wall tubing (0.26 mm wall thickness, model Q-150K 1/8-02-QB481N- 25; Qualtek Electronics). The 2.8-mm instruments with the | Full Paper |
2011 | Karkera, J;Steiner, H;Li, W;Skradski, V;Moser, PL;Riethdorf, S;Reddy, M;Puchalski, T;Safer, K;Prabhakar, U;Pantel, K;Qi, M;Culig, Z; | The anti-interleukin-6 antibody siltuximab down-regulates genes implicated in tumorigenesis in prostate cancer patients from a phase I study | Prostate | 21321981 | PSA, IL‐6R, alpha‐methylacyl‐CoA racemase (AMACR), AR, and Chromogranin A were tested in tumor tissue samples at Qualtek Laboratories (Goleta, CA). Steam heat‐induced epitope retrieval (SHIER) method was used and immunohistochemical procedure was per | Full Paper |
2011 | Lynch, FC; | A method for following patients with retrievable inferior vena cava filters: results and lessons learned from the first 1,100 patients | J Vasc Interv Radiol | 21903414 | Full Paper | |
2011 | Shaw, CM;Scorza, LB;Waybill, PN;Singh, H;Lynch, FC; | Optional vena cava filter use in the elderly population | J Vasc Interv Radiol | 21530308 | Full Paper | |
2011 | Lynch, FC; | Removal of a Günther Tulip filter after 3,006 days | J Vasc Interv Radiol | 21277802 | Full Paper | |
2011 | Maxwell, SA;Cherry, EM;Bayless, KJ; | Akt, 14-3-3ζ, and vimentin mediate a drug-resistant invasive phenotype in diffuse large B-cell lymphoma | Leuk. Lymphoma | 21323512 | Preparation of DLBCL tissue extracts for Western blotting Normal and DLBCL lymphatic tissues were pro- cured through Folio Biosciences (Foliobio.com). Frozen tissue was ground to a powder with a pestle in a mortar while immersed in liquid nitrogen | Full Paper |
2011 | Farris, MH;Duffy, C;Findlay, RH;Olson, JB; | Streptomyces scopuliridis sp. nov., a bacteriocin-producing soil streptomycete | Int. J. Syst. Evol. Microbiol. | 20870885 | ; sequencing facility. Genomic DNA isolated from strain RB72 T using the method of Bollet et al. (1991) was sent to the HudsonAlpha Genomic Services Lab (Huntsville, AL) for Illumina Genome Analyser IIx sequencing. 16S rRNA | Full Paper |
2011 | Lee, LF;Axtell, R;Tu, GH;Logronio, K;Dilley, J;Yu, J;Rickert, M;Han, B;Evering, W;Walker, MG;Shi, J;de Jong, BA;Killestein, J;Polman, CH;Steinman, L;Lin, JC; | IL-7 promotes T(H)1 development and serum IL-7 predicts clinical response to interferon-β in multiple sclerosis | Science translational medicine | 21795588 | Human mononuclear cells were prepared from the buffy coats of samples from healthy adult donors (Stanford Blood Center) or from cord blood purchased from AllCells on Ficoll-Paque gradients. CD4+ T cells were isolated by magnetic bead depletion of CD1 | Full Paper |
2011 | Topp, MS;Kufer, P;Gökbuget, N;Goebeler, M;Klinger, M;Neumann, S;Horst, HA;Raff, T;Viardot, A;Schmid, M;Stelljes, M;Schaich, M;Degenhard, E;Köhne-Volland, R;Brüggemann, M;Ottmann, O;Pfeifer, H;Burmeister, T;Nagorsen, D;Schmidt, M;Lutterbuese, R;Reinhardt, C;Baeuerle, PA;Kneba, M;Einsele, H;Riethmüller, G;Hoelzer, D;Zugmaier, G;Bargou, RC; | Targeted therapy with the T-cell-engaging antibody blinatumomab of chemotherapy-refractory minimal residual disease in B-lineage acute lymphoblastic leukemia patients results in high response rate and prolonged leukemia-free survival | Journal of clinical oncology : official journal of the American Society of Clinical Oncology | 21576633 | Shown is the analysis of DNA fragmentation in B-lineage ALLcells after 48 hours of culture in serum-free medium alone. In this DNA fragmentation assay, intact chromatin and Save | Full Paper |
2011 | Yoneda, A;Kurokawa, T; | A sensitive sandwich ELISA to measure (1→ 3)-β-d-glucan levels in blood | Journal of immunological methods | 2009). 2.7. Measurement of plasma β-glucan. For the measurements of β-glucan level in human plasma, we used commercially available β-glucan-positive human plasma samples purchased from Discovery Life Sciences, Inc | Full Paper | |
2011 | Mayer, JA;Pham, T;Wong, KL;Scoggin, J;Sales, EV; | FISH-based determination of HER2 status in circulating tumor cells isolated with the microfluidic CEE™ platform | Cancer genetics | Peripheral blood was collected under appropriate third-party institutional review board-approved protocols (ConversantBIO, Huntsville, AL; BioOptions, Fullerton, CA) and delivered to Biocept's CLIA/CAP accredited laboratory | Full Paper | |
2011 | Lundgren, JA;Matsushima, K;Lynch, FC;Frankel, H;Cooney, RN; | Angiographic embolization of nonvariceal upper gastrointestinal bleeding: predictors of clinical failure | J Trauma | 21610434 | Full Paper | |
2011 | Balazs, AB;Chen, J;Hong, CM;Rao, DS;Yang, L;Baltimore, D; | Antibody-based protection against HIV infection by vectored immunoprophylaxis | Nature | 22139420 | Human peripheral mononuclear blood cells (AllCells) were thawed from −80 C, expanded in RPMI medium supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin mix( Save | Full Paper |
2011 | Sigal, A;Kim, JT;Balazs, AB;Dekel, E;Mayo, A;Milo, R;Baltimore, D; | Cell-to-cell spread of HIV permits ongoing replication despite antiretroviral therapy | Nature | 21849975 | Anonymous PBMCs or peripheral blood samples were provided by AllCells (PBMCs) or the UCLA Center for AIDS Research (CFAR) Virology Core Lab (peripheral blood). For whole blood, PBMCs were purified by Ficoll gradient using standard techniques. Purifie | Full Paper |
2011 | Doyon, Y;Vo, TD;Mendel, MC;Greenberg, SG;Wang, J;Xia, DF;Miller, JC;Urnov, FD;Gregory, PD;Holmes, MC; | Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures | Nature methods | 21131970 | K562 cells were transfected using the 96-well Nucleofector Kit SF (Lonza) per manufacturer's recommendations. PBMCs (AllCells) were received on ice, centrifuged at 1,000g for 5 min at room temperature (22 °C) and changed to pre-warmed RPMI medium sup | Full Paper |
2011 | Chou, BK;Mali, P;Huang, X;Ye, Z;Dowey, SN;Resar, LM;Zou, C;Zhang, YA;Tong, J;Cheng, L; | Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures | Cell research | 21243013 | f Hematology in Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA 5Cell Therapy Center, Xuanwu Hospital and Capital Medical University, Beijing, China 6AllCells LLC, Emeryville, California, USA *The Joh | Full Paper |
2011 | Lu, SJ;Li, F;Yin, H;Feng, Q;Kimbrel, EA;Hahm, E;Thon, JN;Wang, W;Italiano, JE;Cho, J;Lanza, R; | Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living mice | Cell research | 21221130 | versity of Illinois-Chicago review board. Informed consent was provided according to the Declaration of Helsinki. In some experiments, human blood samples were also obtained from a commercial source (AllCells, Emeryville, CA, USA). For hESC-PLTs, cul | Full Paper |
2011 | Kikushige, Y;Ishikawa, F;Miyamoto, T;Shima, T;Urata, S;Yoshimoto, G;Mori, Y;Iino, T;Yamauchi, T;Eto, T;Niiro, H;Iwasaki, H;Takenaka, K;Akashi, K; | Self-renewing hematopoietic stem cell is the primary target in pathogenesis of human chronic lymphocytic leukemia | Cancer cell | 21840488 | Human age-matched adult bone marrow and peripheral blood cells were obtained from healthy donors or purchased from AllCells Inc. (Emeryville, CA, USA). Informed consent was obtained from all patients and controls in accordance with the Helsinki Decla | Full Paper |
2011 | Panuganti, S; | Towards large-scale production of platelets for transfusion based on ex vivo expansion of megakaryocytes from hematopoietic stem cells | Thesis | Cultures were initiated in T-flasks with previously frozen mPB CD34-selected cells (AllCells; Berkeley, CA or Fred Hutchinson Cancer Research Center; Seattle, WA) and maintained at a concentration of 100,000 to 300,000 cells/ml in X-VIVO 20 (Lonza; W | Full Paper | |
2011 | Carter, CC;McNamara, LA;Onafuwa-Nuga, A;Shackleton, M;Riddell, J;Bixby, D;Savona, MR;Morrison, SJ;Collins, KL; | HIV-1 utilizes the CXCR4 chemokine receptor to infect multipotent hematopoietic stem and progenitor cells | Cell host & microbe | 21402361 | We obtained pre-existing umbilical cord blood lacking subject identifiers after scheduled cesarean section procedures. We obtained fresh whole bone marrow aspirates from a commercial source (AllCells Ltd.). We prepared BMMCs and UCB mononuclear cells | Full Paper |
2011 | Matsuyama, H;Suzuki, HI;Nishimori, H;Noguchi, M;Yao, T;Komatsu, N;Mano, H;Sugimoto, K;Miyazono, K; | miR-135b mediates NPM-ALK-driven oncogenicity and renders IL-17-producing immunophenotype to anaplastic large cell lymphoma | Blood | 22042699 | Many transformed lymphoma cells show immune-phenotypes resembling the corresponding normal lymphocytes, which gives a guide for proper diagnosis and presents promising Save;Human normal peripheral blood pan T lymphocytes were purchased from AllCell | Full Paper |
2011 | Aguila, JR;Liao, W;Yang, J;Avila, C;Hagag, N;Senzel, L;Ma, Y; | SALL4 is a robust stimulator for the expansion of hematopoietic stem cells | Blood | 21602528 | Normal human bone marrow CD34 + cells were purchased (AllCells) and single-cell suspensions were generated in StemSpan SFEM (StemCell Technologies) for 24 hours. Next, 10 5 Save | Full Paper |
2011 | Iancu-Rubin, C;Gajzer, D;Tripodi, J;Najfeld, V;Gordon, RE;Hoffman, R;Atweh, GF; | Down-regulation of stathmin expression is required for megakaryocyte maturation and platelet production | Blood | 21364187 | Human MKs were generated in liquid culture from peripheral blood (PB)-derived CD34 + cells purchased from AllCells and suspended in Iscove modified Dulbecco medium (Invitrogen) Save | Full Paper |
2011 | Liu, ZJ;Italiano, J;Ferrer-Marin, F;Gutti, R;Bailey, M;Poterjoy, B;Rimsza, L;Sola-Visner, M; | Developmental differences in megakaryocytopoiesis are associated with up-regulated TPO signaling through mTOR and elevated GATA-1 levels in neonatal megakaryocytes | Blood | 21304100 | Human umbilical CB-CD34+ cells were purchased from AllCells. G-CSF-mobilized adult peripheral blood (PB) CD34+ cells were purchased from the Fred Hutchinson Cancer Institute (Seattle, WA). CD34+ cells from either source were cultured in serum-free me | Full Paper |
2011 | Adams, GN;LaRusch, GA;Stavrou, E;Zhou, Y;Nieman, MT;Jacobs, GH;Cui, Y;Lu, Y;Jain, MK;Mahdi, F;Shariat-Madar, Z;Okada, Y;D'Alecy, LG;Schmaier, AH; | Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosis | Blood | 21297000 | HUVECs were purchased from AllCells and grown on gelatin-coated plates in CSC media (Cells Systems). Cells were grown to near-confluence in 6-well plates and transfected with Lipofectamine 2000 reagent (Invitrogen) in OPTI-MEM media (Invitrogen) over | Full Paper |
2011 | Hu, K;Yu, J;Suknuntha, K;Tian, S;Montgomery, K;Choi, KD;Stewart, R;Thomson, JA;Slukvin, II; | Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells | Blood | 21296996 | Frozen CB mononuclear cells were obtained from AllCells. BM mononuclear cells from normal donors and from a patient with CML in the chronic phase were purchased from AllCells. Save | Full Paper |
2011 | Karp, JE;Smith, BD;Resar, LS;Greer, JM;Blackford, A;Zhao, M;Moton-Nelson, D;Alino, K;Levis, MJ;Gore, SD;Joseph, B;Carraway, H;McDevitt, MA;Bagain, L;Mackey, K;Briel, J;Doyle, LA;Wright, JJ;Rudek, MA; | Phase 1 and pharmacokinetic study of bolus-infusion flavopiridol followed by cytosine arabinoside and mitoxantrone for acute leukemias | Blood | 21239698 | Flavopiridol is a protein bound, cytotoxic, cyclin-dependent kinase inhibitor. Flavopiridol given by 1-hour bolus at 50 mg/m 2 daily 3 times followed by cytosine arabinoside and Save | Full Paper |
2011 | Huang, X;Gschweng, E;Van Handel, B;Cheng, D;Mikkola, HK;Witte, ON; | Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cells | Blood | 21163928 | The analyzed samples include H1 hESCs, day 7 and 15 H1 EB cells, and a mixture of 3 adult bone marrow CD34 + cells (AllCells). The data were analyzed with SDS RQ Manager 1.2 ( Save | Full Paper |
2011 | Lugthart, S;Figueroa, ME;Bindels, E;Skrabanek, L;Valk, PJ;Li, Y;Meyer, S;Erpelinck-Verschueren, C;Greally, J;Löwenberg, B;Melnick, A;Delwel, R; | Aberrant DNA hypermethylation signature in acute myeloid leukemia directed by EVI1 | Blood | 20855866 | healthy donors, 4 of which were acquired from the Translational Trials Development and Support Laboratory, Cincinnati Children's Hospital, and 4 of which were purchased from Allcells Save | Full Paper |
2011 | Mack, AA;Kroboth, S;Rajesh, D;Wang, WB; | Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors | PloS one | 22132178 | a permit number or approval ID since mouse is not a protected species. Processing whole blood samples Peripheral (PB.1 and PB.2) and cord (CB.1 and CB.2) blood-derived CD34+ cells were obtained from AllCells (Emeryville, CA USA). Blood collections w | Full Paper |
2011 | Moorefield, EC;McKee, EE;Solchaga, L;Orlando, G;Yoo, JJ;Walker, S;Furth, ME;Bishop, CE; | Cloned, CD117 selected human amniotic fluid stem cells are capable of modulating the immune response | PloS one | 22046303 | lates for Elispot (Millipore, Billerica, MA) were coated with human interferon-γ (IFN-γ) capture antibody (2G1; Endogen, Rockford, IL). Peripheral blood mononuclear cells (PBMCs) were acquired from AllCells (Emeryville, CA), activated with 5 µg/ml PH | Full Paper |
2011 | Corbin, AS;Agarwal, A;Loriaux, M;Cortes, J;Deininger, MW;Druker, BJ; | Human chronic myeloid leukemia stem cells are insensitive to imatinib despite inhibition of BCR-ABL activity | The Journal of clinical investigation | 21157039 | Imatinib therapy, which targets the oncogene product BCR-ABL, has transformed chronic myeloid leukemia (CML) from a life-threatening disease into a chronic condition. Most patients, Save | Full Paper |
2011 | Rowe, DD; | Secreted factors from human umbilical cord blood cells protect oligodendrocytes from ischemic insult | Thesis | that prevented OL-HUCB cell contact but was permeable to media and soluble factors. Cryopreserved HUCB cells (ALLCELLS, Emeryville, CA) were rapidly thawed, washed, pelleted to remove the cryopreservatives and re-suspended in 10 ml DMEM with glucose | Full Paper | |
2011 | Zhou, L;McMahon, C;Bhagat, T;Alencar, C;Yu, Y;Fazzari, M;Sohal, D;Heuck, C;Gundabolu, K;Ng, C;Mo, Y;Shen, W;Wickrema, A;Kong, G;Friedman, E;Sokol, L;Mantzaris, I;Mantzaris, G;Pellagatti, A;Boultwood, J;Platanias, LC;Steidl, U;Yan, L;Yingling, JM;Lahn, MM;List, A;Bitzer, M;Verma, A; | Reduced SMAD7 leads to overactivation of TGF-beta signaling in MDS that can be reversed by a specific inhibitor of TGF-beta receptor I kinase | Cancer research | 21189329 | Bone marrow CD34+ cells from various normal donors were also obtained from AllCells (Emeryville, CA). K562 and HS-5 cell lines were purchased from ATCC. Erythropoietin and Transforming growth factor-β1 (TGF-β1) were bought from R&D Systems (Minneapol | Full Paper |
2011 | Nicolaides, TP;Li, H;Solomon, DA;Hariono, S;Hashizume, R;Barkovich, K;Baker, SJ;Paugh, BS;Jones, C;Forshew, T;Hindley, GF;Hodgson, JG;Kim, JS;Rowitch, DH;Weiss, WA;Waldman, TA;James, CD; | Targeted therapy for BRAFV600E malignant astrocytoma | Clinical cancer research : an official journal of the American Association for Cancer Research | 22038996 | Normal human astrocytes (NHA) were obtained from Clonetics and AllCells. All cell sources were authenticated through DNA fingerprinting using the Promega Powerplex platform. Save | Full Paper |
2011 | Kurtzberg, LS;Roth, S;Krumbholz, R;Crawford, J;Bormann, C;Dunham, S;Yao, M;Rouleau, C;Bagley, RG;Yu, XJ;Wang, F;Schmid, SM;Lavoie, EJ;Teicher, BA; | Genz-644282, a novel non-camptothecin topoisomerase I inhibitor for cancer treatment | Clinical cancer research : an official journal of the American Association for Cancer Research | 21415217 | Viable human bone marrow cells were purchased as frozen vials from AllCells, LLC., via StemCell Technologies (#ABM009). They were prepared with a DNase treatment (#07900; Save | Full Paper |
2011 | Arastu-Kapur, S;Anderl, JL;Kraus, M;Parlati, F;Shenk, KD;Lee, SJ;Muchamuel, T;Bennett, MK;Driessen, C;Ball, AJ;Kirk, CJ; | Nonproteasomal targets of the proteasome inhibitors bortezomib and carfilzomib: a link to clinical adverse events | Clinical cancer research : an official journal of the American Association for Cancer Research | 21364033 | Purpose: Bortezomib (Velcade), a dipeptide boronate 20S proteasome inhibitor and an approved treatment option for multiple myeloma, is associated with a treatment-emergent, painful Save | Full Paper |
2011 | Chao, MP;Alizadeh, AA;Tang, C;Jan, M;Weissman-Tsukamoto, R;Zhao, F;Park, CY;Weissman, IL;Majeti, R; | Therapeutic antibody targeting of CD47 eliminates human acute lymphoblastic leukemia | Cancer research | 21177380 | Human normal bone marrow (NBM) cells were purchased from AllCells Inc. Human ALL samples were obtained from patients at the Stanford University Medical Center, with informed Save | Full Paper |
2011 | Pang, WW;Price, EA;Sahoo, D;Beerman, I;Maloney, WJ;Rossi, DJ;Schrier, SL;Weissman, IL; | Human bone marrow hematopoietic stem cells are increased in frequency and myeloid-biased with age | Proceedings of the National Academy of Sciences of the United States of America | 22123971 | In the human hematopoietic system, aging is associated with decreased bone marrow cellularity, decreased adaptive immune system function, and increased incidence of anemia and Save;Normal young human bone marrow mononuclear cells were purchased fro | Full Paper |
2011 | Jan, M;Chao, MP;Cha, AC;Alizadeh, AA;Gentles, AJ;Weissman, IL;Majeti, R; | Prospective separation of normal and leukemic stem cells based on differential expression of TIM3, a human acute myeloid leukemia stem cell marker | Proceedings of the National Academy of Sciences of the United States of America | 21383193 | Normal human bone marrow mononuclear cells were purchased from AllCells. Human AML samples were obtained from patients at the Stanford Medical Center with informed consent, Save | Full Paper |
2011 | Sahler, J;Bernard, JJ;Spinelli, SL;Blumberg, N;Phipps, RP; | The Feverfew plant-derived compound, parthenolide enhances platelet production and attenuates platelet activation through NF-κB inhibition | Thrombosis research | 21272923 | Adapted from previous procedures [4], human CD34+ cord blood cells were purchased from Allcells (Emeryville, CA) and first plated at 2.5 × 10 5 cells/mL in IMDM supplemented with Save | Full Paper |
2011 | Nahimana, A;Aubry, D;Lagopoulos, L;Greaney, P;Attinger, A;Demotz, S;Dawson, KM;Schapira, M;Tschopp, J;Dupuis, M;Duchosal, MA; | A novel potent Fas agonist for selective depletion of tumor cells in hematopoietic transplants | Blood cancer journal | 22829095 | lture and analysis conditions Human CD34+ cells from cord blood (CB), mobilized peripheral blood (mPB) or bone marrow (BM) were obtained unpurified (mPB) or already purified (CB, mPB, BM) from either AllCells, LLC (San Mateo, CA, USA), or purified us | Full Paper |
2011 | Hart, S;Goh, KC;Novotny-Diermayr, V;Tan, YC;Madan, B;Amalini, C;Ong, LC;Kheng, B;Cheong, A;Zhou, J;Chng, WJ;Wood, JM; | Pacritinib (SB1518), a JAK2/FLT3 inhibitor for the treatment of acute myeloid leukemia | Blood cancer journal | 22829080 | Primary cells, either peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex ( Save | Full Paper |
2011 | Nagao, R;Ashihara, E;Kimura, S;Strovel, JW;Yao, H;Takeuchi, M;Tanaka, R;Hayashi, Y;Hirai, H;Padia, J;Strand, K;Maekawa, T; | Growth inhibition of imatinib-resistant CML cells with the T315I mutation and hypoxia-adaptation by AV65--a novel Wnt/β-catenin signaling inhibitor | Cancer letters | 21906872 | Bone marrow (BM) mononuclear cells obtained from CML patients and healthy volunteers (ALLCells, Emeryville, CA) were plated in duplicate in MethoCult H4434 Classic (StemCell Technologies Inc, Vancouver, Canada) and cultured at 37 °C in 5% CO2. After | Full Paper |
2011 | Gao, N;Budhraja, A;Cheng, S;Liu, EH;Chen, J;Yang, Z;Chen, D;Zhang, Z;Shi, X; | Phenethyl isothiocyanate exhibits antileukemic activity in vitro and in vivo by inactivation of Akt and activation of JNK pathways | Cell death & disease | 21472003 | Fresh normal peripheral blood mononuclear cells were purchased from Allcells (Emeryvill, CA, USA). After washing and enumerating, cells were suspended at 8 × 10 5 /ml before Save | Full Paper |
2011 | Yang, L;Wang, H;Kornblau, SM;Graber, DA;Zhang, N;Matthews, JA;Wang, M;Weber, DM;Thomas, SK;Shah, JJ;Zhang, L;Lu, G;Zhao, M;Muddasani, R;Yoo, SY;Baggerly, KA;Orlowski, RZ; | Evidence of a role for the novel zinc-finger transcription factor ZKSCAN3 in modulating Cyclin D2 expression in multiple myeloma | Oncogene | 21057542 | The current studies on deidentified aliquots were performed according to an Institutional Review Board-approved protocol. Normal human CD19+ B-cell total RNA was from Miltenyi Biotec, Inc., and plasma cell cDNA was from AllCells LLC. (Berkeley, CA) | Full Paper |
2011 | Yu, X;McCarthy, PJ;Lim, HJ;Iempridee, T;Kraus, RJ;Gorlen, DA;Mertz, JE; | The ZIIR element of the Epstein-Barr virus BZLF1 promoter plays a central role in establishment and maintenance of viral latency | Journal of virology | 21389123 | Fresh human primary B cells from peripheral blood of healthy donors were purchased from AllCells (Emeryville, CA). The B cells were infected the day they arrived by incubation at 4C Save | Full Paper |
2011 | Sagawa, M;Shimizu, T;Fukushima, N;Kinoshita, Y;Ohizumi, I;Uno, S;Kikuchi, Y;Ikeda, Y;Yamada-Okabe, H;Kizaki, M; | A new disulfide-linked dimer of a single-chain antibody fragment against human CD47 induces apoptosis in lymphoid malignant cells via the hypoxia inducible factor-1α pathway | Cancer science | 21401803 | CD47 belongs to the immunoglobulin superfamily and is associated with β‐integrins. Recently it was reported that CD47 ligation rapidly induces apoptosis in B‐chronic lymphocytic Save | Full Paper |
2011 | Takeuchi, M;Ashihara, E;Yamazaki, Y;Kimura, S;Nakagawa, Y;Tanaka, R;Yao, H;Nagao, R;Hayashi, Y;Hirai, H;Maekawa, T; | Rakicidin A effectively induces apoptosis in hypoxia adapted Bcr-Abl positive leukemic cells | Cancer science | 21166958 | Bone marrow cells from two normal volunteers and CML patients in the chronic phase before imatinib treatment were purchased from AllCells Inc. (Emeryville, CA, USA). Thawed Save | Full Paper |
2011 | Su, WR;Zhang, QZ;Shi, SH;Nguyen, AL;Le, AD; | Human gingiva-derived mesenchymal stromal cells attenuate contact hypersensitivity via prostaglandin E2-dependent mechanisms | Stem cells (Dayton, Ohio) | 21987520 | CD14+ cells were negatively selected from human peripheral blood mononuclear cells (PBMCs) (AllCells LLC, Emeryville, CA, http://www.allcells.com) using the MACS CD14 MicroBeads (Monocyte Isolation Kit, Miltenyi Biotec, Auburn, CA, http://www.milteny | Full Paper |
2011 | Bissels, U;Wild, S;Tomiuk, S;Hafner, M;Scheel, H;Mihailovic, A;Choi, YH;Tuschl, T;Bosio, A; | Combined characterization of microRNA and mRNA profiles delineates early differentiation pathways of CD133+ and CD34+ hematopoietic stem and progenitor cells | Stem cells (Dayton, Ohio) | 21394831 | tenyi Biotec) using the negative fraction of the first separation. The gating of the separated cells was performed according to ISHAGE. CD133+ cells from mobilized peripheral blood were obtained from AllCells LLC. RNA Sources Total RNA was isolated | Full Paper |
2011 | Mendelson, A;Frank, E;Allred, C;Jones, E;Chen, M;Zhao, W;Mao, JJ; | Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cells in vitro | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 21746864 | Human bone marrow MSCs (AllCells, Emeryville, CA, USA), human ASCs, and human SSCs were isolated from fresh adult donor samples using previously established methods (27-31). MSCs were purified from bone marrow using the RosetteSep Human Mesenchymal S | Full Paper |
2011 | Gao, N;Budhraja, A;Cheng, S;Liu, EH;Huang, C;Chen, J;Yang, Z;Chen, D;Zhang, Z;Shi, X; | Interruption of the MEK/ERK signaling cascade promotes dihydroartemisinin-induced apoptosis in vitro and in vivo | Apoptosis : an international journal on programmed cell death | 21336837 | Fresh normal peripheral blood mononuclear cells were purchased from Allcells (Emeryville, CA, USA). After washing and enumerating as described for mononuclear cells, cells were Save | Full Paper |
2011 | Zhou, L;Opalinska, J;Sohal, D;Yu, Y;Mo, Y;Bhagat, T;Abdel-Wahab, O;Fazzari, M;Figueroa, M;Alencar, C;Zhang, J;Kambhampati, S;Parmar, S;Nischal, S;Hueck, C;Suzuki, M;Freidman, E;Pellagatti, A;Boultwood, J;Steidl, U;Sauthararajah, Y;Yajnik, V;McMahon, C;Gore, SD;Platanias, LC;Levine, R;Melnick, A;Wickrema, A;Greally, JM;Verma, A; | Aberrant epigenetic and genetic marks are seen in myelodysplastic leukocytes and reveal Dock4 as a candidate pathogenic gene on chromosome 7q | The Journal of biological chemistry | 21532034 | 106 CD34+ cells (AllCells) were thawed, cultured for 2 h, resuspended in 100 μl of human CD34+ Nucleofection solution (Amaxa), then transferred into cuvettes, and electroporated using program U008. CD34+ cells were collected and cultured in 24-well p | Full Paper |
2011 | Froelich, S;Tai, A;Kennedy, K;Zubair, A;Wang, P; | Pseudotyping lentiviral vectors with aura virus envelope glycoproteins for DC-SIGN-mediated transduction of dendritic cells | Human gene therapy | 21452926 | Peripheral blood mononuclear cells (PBMCs) from healthy human donors were purchased from AllCells. PBMCs were allowed to adhere for 2 hr on non-tissue culture-treated 24-well plates and washed extensively. Adherent monocytes were plated at a density | Full Paper |
2011 | Jiang, X;Christopherson, GT;Mao, HQ; | The effect of nanofibre surface amine density and conjugate structure on the adhesion and proliferation of human haematopoietic progenitor cells | Interface focus | 23050077 | Frozen human UCB CD133+ cells were purchased from AllCells, LLC (Emeryville, CA, USA). The CD133+ purity in the post-thawed HSPCs was determined by flow cytometry to be 97 per cent and the viability was determined by Trypan blue exclusion assay to be | Full Paper |
2011 | Zhu, J;Shimizu, E;Zhang, X;Partridge, NC;Qin, L; | EGFR signaling suppresses osteoblast differentiation and inhibits expression of master osteoblastic transcription factors Runx2 and Osterix | Journal of cellular biochemistry | 21381079 | The epidermal growth factor receptor (EGFR) and its ligands regulate key processes of cell biology, such as proliferation, survival, differentiation, migration, and tumorigenesis. We Save | Full Paper |
2011 | Khoury, M;Drake, A;Chen, Q;Dong, D;Leskov, I;Fragoso, MF;Li, Y;Iliopoulou, BP;Hwang, W;Lodish, HF;Chen, J; | Mesenchymal stem cells secreting angiopoietin-like-5 support efficient expansion of human hematopoietic stem cells without compromising their repopulating potential | Stem cells and development | 21142526 | Purified human CD133 + cord blood cells were purchased from AllCells. Alternatively, umbilical cord blood was obtained from the National Disease Research Interchange or the Save | Full Paper |
2011 | Hong, L;Zhang, G;Sultana, H;Yu, Y;Wei, Z; | The effects of 17-β estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitro | Stem cells and development | 20735179 | Fresh human bone marrow donated by 12 healthy donors (6 males and 6 females, average age = 27.4 ± 6.1) were purchased (Allcells, Emeryville, CA). Mononuclear cells were isolated using density gradient centrifugation (Histopaque-1.077; Sigma, St. Loui | Full Paper |
2011 | Kunisato, A;Wakatsuki, M;Shinba, H;Ota, T;Ishida, I;Nagao, K; | Direct generation of induced pluripotent stem cells from human nonmobilized blood | Stem cells and development | 20497033 | BM and PB MNCs were obtained from Allcells (ABM007F and PB003F each). Frozen BM MNCs purchased from Allcells were collected from a 25-year-old male donor (lot#BM1705), a Save | Full Paper |
2011 | Basu, J;Genheimer, CW;Sangha, N;Quinlan, SF;Guthrie, KI;Kelley, R;Ilagan, RM;Jain, D;Bertram, T;Ludlow, JW; | Organ specific regenerative markers in peri-organ adipose: kidney | Lipids in health and disease | 21957910 | CD34+ GCSF mobilized/non-mobilized peripheral blood mononuclear cell cDNA was purchased from AllCells LLC. Fetal and adult hepatocyte cDNA and keratinocyte cDNA were Save | Full Paper |
2011 | Gupta, MS;Cooper, ES;Nicoll, SB; | Transforming growth factor-beta 3 stimulates cartilage matrix elaboration by human marrow-derived stromal cells encapsulated in photocrosslinked carboxymethylcellulose hydrogels: potential for nucleus pulposus replacement | Tissue engineering. Part A | 21707438 | hMSCs were isolated from the bone marrow of a 37-year-old man (AllCells, Emeryville, CA) using enriched density centrifugation. Briefly, an hMSC Enrichment Cocktail (StemCell Technologies, Vancouver, BC, Canada)44 was added to the bone marrow and inc | Full Paper |
2011 | McWhirter, J;Noguchi, H;Gompper, G; | Deformation and clustering of red blood cells in microcapillary flows | Soft Matter | Trajectories are shown in a co-moving frame, ie relative to the center-of-mass of allcells. Solid and dashed lines represent the cm of RBCs and their periodic images, respectively. Save | Full Paper | |
2011 | Vanella, L;Kim, DH;Sodhi, K;Barbagallo, I;Burgess, AP;Falck, JR;Schwartzman, ML;Abraham, NG; | Crosstalk between EET and HO-1 downregulates Bach1 and adipogenic marker expression in mesenchymal stem cell derived adipocytes | Prostaglandins & other lipid mediators | 21821145 | Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 10% heat inactivated fetal bovi | Full Paper |
2011 | MacEneaney, OJ;Connick, E;DeSouza, CA; | Effects of HIV-1 gp120 and protease inhibitors on apoptotic susceptibility of CD34+ hematopoietic progenitor cells | Journal of acquired immune deficiency syndromes (1999) | 21233630 | Freshly-isolated CD34+ cells from healthy G-CSF-treated human donors (n=3) were obtained from an independent supplier (Allcells, LLC, Berkeley, CA). Donors tested negative for HIV-1, hepatitis B and hepatitis C. Viability of cells determined by trypa | Full Paper |
2011 | He, JQ;Vu, DM;Hunt, G;Chugh, A;Bhatnagar, A;Bolli, R; | Human cardiac stem cells isolated from atrial appendages stably express c-kit | PloS one | 22140461 | Human bone marrow cells were purchased from AllCells (Emeryville, CA). Total RNA of human carotid artery was bought from Agilent Technologies (Santa Clara, CA). Cells were Save | Full Paper |
2011 | Kobayashi, DT;Olson, RJ;Sly, L;Swanson, CJ;Chung, B;Naryshkin, N;Narasimhan, J;Bhattacharyya, A;Mullenix, M;Chen, KS; | Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analyses | PloS one | 21904622 | For the dilutional linearity experiment, six lots of control PBMCs were purchased from AllCells for further assay testing. After thawing and lysis, PBMCs were serially diluted 4-fold and Save | Full Paper |
2011 | Froelich, S;Tai, A;Kennedy, K;Zubair, A;Wang, P; | Virus-receptor mediated transduction of dendritic cells by lentiviruses enveloped with glycoproteins derived from Semliki Forest virus | PloS one | 21738680 | th 293T.DCSIGN cells for 8 hrs before changing to fresh D10 medium. Transduction of human PBMC-derived DCs Peripheral blood mononuclear cells (PBMC) from two healthy human donors were purchased from AllCells (Emeryville, CA). PBMC were differentiate | Full Paper |
2011 | Danner, R;Chaudhari, SN;Rosenberger, J;Surls, J;Richie, TL;Brumeanu, TD;Casares, S; | Expression of HLA class II molecules in humanized NOD.Rag1KO.IL2RgcKO mice is critical for development and function of human T and B cells | PloS one | 21611197 | ) Tg.NOD.Rag1KO.IL2RγcKO (referred as DRAG) mice were maintained in the pathogen-free WRAIR Veterinary Medicine Facility. Infusion of mice with HSC Umbilical cord bloods (UCB) were purchased from AllCells (Emeryville,CA) and Promocell (Heidelberg, G | Full Paper |
2011 | Drake, AC;Khoury, M;Leskov, I;Iliopoulou, BP;Fragoso, M;Lodish, H;Chen, J; | Human CD34+ CD133+ hematopoietic stem cells cultured with growth factors including Angptl5 efficiently engraft adult NOD-SCID Il2rγ-/- (NSG) mice | PloS one | 21559522 | rch. The MIT committee on animal care (MIT CAC) approved the research as part of animal protocol 0310-027-13. Cell isolation and culture Purified CD133+ human umbilical cord HSCs were purchased from AllCells (California) or National Disease Research | Full Paper |
2011 | Burridge, PW;Thompson, S;Millrod, MA;Weinberg, S;Yuan, X;Peters, A;Mahairaki, V;Koliatsos, VE;Tung, L;Zambidis, ET; | A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability | PloS one | 21494607 | For generation of the non-viral CD34+ cord blood-derived hiPSC lines (CBiPSC6.2, CBiPSC6.11, CBiPSC6.13 and CBiPSC19.11), 0.5-1×106 human CD34+ cord blood cells (AllCells, Emeryville, CA, http://www.allcells.com) were expanded in StemSpan-SFEM | Full Paper |
2011 | Martellini, JA;Cole, AL;Svoboda, P;Stuchlik, O;Chen, LM;Chai, KX;Gangrade, BK;Sørensen, OE;Pohl, J;Cole, AM; | HIV-1 enhancing effect of prostatic acid phosphatase peptides is reduced in human seminal plasma | PloS one | 21283773 | obtained from the National Institutes of Health AIDS Research and Reference Reagent Program (Germantown, MD, USA), and Peripheral Blood Mononuclear Cells (PBMCs) were obtained from healthy donors by AllCells, LLC (Emeryville, CA, USA). TZM-bl cells w | Full Paper |
2011 | Gupta, A;Le, A;Belinka, BA;Kachlany, SC; | In vitro synergism between LFA-1 targeting leukotoxin (Leukothera™) and standard chemotherapeutic agents in leukemia cells | Leukemia research | 21664691 | and maintained in RPMI 1640 medium with 10% fetal bovine serum (Invitrogen, Carlsbad, CA) at 37 °C, 5% CO2. 2.2. Primary cells Frozen primary human cells were purchased from AllCells, LLC. (Emeryville, CA). Viability of these primary cells was 90% | Full Paper |
2011 | Bouchez, LC;Boitano, AE;de Lichtervelde, L;Romeo, R;Cooke, MP;Schultz, PG; | Small-molecule regulators of human stem cell self-renewal | Chembiochem : a European journal of chemical biology | 21381174 | screen: The assay was performed on purified human CD34 + cells from granulocyte colony-stimulating factor-mobilized peripheral blood from normal donors (supplied by AllCells, Save | Full Paper |
2011 | Fukumoto, R;Kiang, JG; | Geldanamycin analog 17-DMAG limits apoptosis in human peripheral blood cells by inhibition of p53 activation and its interaction with heat-shock protein 90 kDa after exposure to ionizing radiation | Radiation research | 21663398 | Exposure to ionizing radiation induces p53, and its inhibition improves mouse survival. We tested the effect of 17-dimethylamino-ethylamino-17-demethoxygeldanamycin (17-DMAG) on Save | Full Paper |
2011 | Pogosova-Agadjanyan, EL;Fan, W;Georges, GE;Schwartz, JL;Kepler, CM;Lee, H;Suchanek, AL;Cronk, MR;Brumbaugh, A;Engel, JH;Yukawa, M;Zhao, LP;Heimfeld, S;Stirewalt, DL; | Identification of radiation-induced expression changes in nonimmortalized human T cells | Radiation research | 21268710 | Peripheral blood samples were collected from healthy volunteer donors at the Fred Hutchinson Cancer Research Center (FHCRC) or purchased from a commercial vendor (AllCells, Emeryville, CA). All samples were obtained under Institutional Review Board ( | Full Paper |
2011 | Bagley, RG;Kurtzberg, L;Rouleau, C;Yao, M;Teicher, BA; | Erufosine, an alkylphosphocholine, with differential toxicity to human cancer cells and bone marrow cells | Cancer chemotherapy and pharmacology | 21526352 | and compounds were added to each well of a 6-well plate in a final volume of 3 mls; RPMI8226 multiple myeloma cells and compounds were added to 3 mls Methocult medium (AllCells Save | Full Paper |
2011 | Hong, L;Krishnamachari, Y;Seabold, D;Joshi, V;Schneider, G;Salem, AK; | Intracellular release of 17-β estradiol from cationic polyamidoamine dendrimer surface-modified poly (lactic-co-glycolic acid) microparticles improves osteogenic differentiation of human mesenchymal stromal cells | Tissue engineering. Part C, Methods | 20883116 | and solvents were purchased from Sigma. All the solvents, including ethyl acetate and dichloromethane (DCM) and acetonitrile, were of high performance liquid chromatography (HPLC) analytical grade. Fresh human bone marrow was purchased from Allcells. | Full Paper |
2011 | McNeish, J;Mathieu, A;Dempsey, B; | Book Review: Bringing stem cell science to the classrooms | Regenerative Medicine | from a small biotechnology company, ReachBio (Seatttle, WA, USA). ReachBio is a contract of clients", so education is clearly a passion at ReachBio. As much of the focus of the text is Save | Full Paper | |
2011 | Nelson, DM;Joseph, B;Hillion, J;Segal, J;Karp, JE;Resar, LM; | Flavopiridol induces BCL-2 expression and represses oncogenic transcription factors in leukemic blasts from adults with refractory acute myeloid leukemia | Leukemia & lymphoma | 21728742 | from the peripheral blood blasts were compared to gene expression in CD34+ hematopoietic stem cells (HSCs); mRNA levels from the HSCs were arbitrarily assigned a value of 1.0. HSC RNA was obtained from a commercial source (ALLCELLS, CA, USA) | Full Paper |
2011 | Ma, K;Fox, L;Shi, G;Shen, J;Liu, Q;Pappas, JD;Cheng, J;Qu, T; | Generation of neural stem cell-like cells from bone marrow-derived human mesenchymal stem cells | Neurological research | 22196762 | The hMSCs from four donors were obtained from AllCells LLC (Emeryville, CA, USA) and Cambrex Bioscience (Walkersville, MD, USA). These hMSCs are negative for surface markers associated with hematopoietic cells (e.g. CD11b, CD33, CD34, and CD133) and | Full Paper |
2011 | Wang, DY;Chang, CW;Lagacé, RE;Oldroyd, NJ;Hennessy, LK; | Development and validation of the AmpFℓSTR® Identifiler® Direct PCR Amplification Kit: a multiplex assay for the direct amplification of single-source samples | Journal of forensic sciences | 21418220 | the performance of the Identifiler Direct Kit on buccal samples deposited on Indicating FTA cards, white blood cells (WBCs) from three individuals were purchased from AllCells, Save | Full Paper |
2011 | Shah, BS;Mao, JJ; | Labeling of mesenchymal stem cells with bioconjugated quantum dots | Methods in molecular biology (Clifton, N.J.) | 21153373 | Bone marrow samples from healthy human donors (AllCells, Berkeley, CA) 2. RosetteSepTM (Stem Cell Technologies, Vancouver, BC, Canada) 3. 35-mm petri dishes (Falcon, Becton Dickinson Labware, Franklin Lakes, NJ) 4. DMEM: Dulbecco’s Modified Eagle’ | Full Paper |
2011 | Li, Z;Chen, J; | In vitro functional study of miR-126 in leukemia | Methods in molecular biology (Clifton, N.J.) | 20931398 | Genomic DNA template isolated from human normal bone marrow mononuclear cells purchased from AllCells (Emeryville, CA). 3. MSCVpuro (Clontech, Mountain View, CA) is a retroviral vector for cloning and gene expression. 4. AML1-ETO fusion gene in a v | Full Paper |
2011 | Kanjevac, T;Milovanovic, M;Volarevic, V;Arsenijevic, N; | Fluoride release from glass ionomer cements correlates with the necrotic death of human dental pulp stem cells | Serbian Journal of Experimental and Clinical Research | Human SHEDs were purchesed from AllCells (Emeryville, California USA). The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS, 100 IU/mL penicillin G and 100 μg/mL streptomycin | Full Paper | |
2011 | Bassan, R;Rohatiner, A;Lerede, T;Carter, M;Di Bona, E;Pogliani, E;Rossi, G;Fabris, P;Morandi, S;Lambertenghi-Deliliers, G;Casula, P;Vespignani, M;Lister, T;Barbui, T; | Early Application of Anthracyclines in Adult Acute Lymphoblastic Leukemia (ALL) | Haematology and Blood Transfusion / Hämatologie und Bluttransfusion | However, because IDR resistance may be dose-dependent, as ascertained by in vitro studies on MDRl+ ALLcells [17], increaseddose IDR may find a place in the treatment of patients Save | Full Paper | |
2011 | Larson, R;Dodge, R;Szatrowski, T;Frankel, S; | Results of Recent Clinical Trials in Acute Lymphoblastic Leukemia by the Cancer and Leukemia Group B | Haematology and Blood Transfusion / Hämatologie und Bluttransfusion | Rapid depletion of asparagine results in selective killing of ALLcells since normal cells are able to synthesize asparagine. Because asparaginase is not markedly myelosuppressive, it is Save | Full Paper | |
2011 | Bissels, U; | Combined analysis of microRNA and mRNA signatures in human hematopoietic stem and progenitor cells using a novel microarray quantification system | Thesis | Granulocyte-colony stimulating factor (G-CSF) mobilized peripheral blood was obtained from healthy donors after informed consent (Allcells, Berkley, USA) and CD133+ cells were Save | Full Paper | |
2011 | Milewski, ME; | The Angiogenic effect of Erythropoietin on Stem Cells In-Vitro | Thesis | HUVEC cells (p-10) were obtained from Allcells, LLC (Emeryville, CA). The cells were removed from the -80°C and placed in a 37°C water bath until thawed. 10 ml of HUVEC media (Allcells, Emeryville, CA) was added to the cells and centrifuged for 5 min | Full Paper | |
2011 | Stewart, HRA; | A novel reporter system where gene knockdown is used to assay intercellular siRNA transfer | Thesis | Mesenchymal stem cells were harvested from bone marrow aspirates purchased from Lonza and AllCells. To isolate human MSCs, nucleated cells were recovered from the 10ml bone marrow aspirates with a density gradient (Ficoll-Paque) and resuspended in co | Full Paper | |
2011 | KC, P; | Proteomic Analysis of Human Mesenchymal Stem Cell Aging during serial sub-culture | Thesis | Human bone marrow was purchased from AllCells, LLC, Emeryvill, CA from a 28 years old donor. Human mesenchymal stem cells were isolated following RosetteSep method and expanded in culture using CFU-F assay. Fluorescence-activated cell sorting (FACS) | Full Paper | |
2011 | Stosich, MS; | Micro-CT evaluation of MSC/HUVEC/HSC-mediated angiogenesis in large PCL-βTCP constructs implanted in nude mice | Thesis | For in vivo study, fresh bone marrow samples of anonymous adult male donors were purchased from AllCells (Berkeley, CA), and used to isolate human mesenchymal stem cells ( Save | Full Paper | |
2011 | Pang, WW;Park, CY;Greenberg, PL; | Separation of normal and aberrant hematopoietic stem cells in myelodysplastic syndrome by CD69 expression | Thesis | Human Samples. Normal young human bone marrow mononuclear cells were purchased from AllCells, Inc. (Emeryville, CA, USA). Normal young and old human bone marrow samples were obtained from hematologically normal donors at the Stanford Medical Center w | Full Paper | |
2011 | Ramos, JH; | Phenotypic and molecular characterization of a human adult bone marrow committed T cell progenitor | Thesis | Human mononuclear cells obtained from fresh, normal adult bone marrow were purchased from AllCells, LLC. (Emeryville, CA). Donors were between the ages of 18 and 55 years, 110 lbs, in good health, and screened for HIV, Hepatitis B and Hepatitis C bef | Full Paper | |
2011 | Thacker, SG; | Type I Interferons and the Development of Impaired Vascular Function and Repair in Human and Murine Lupus | Thesis | lU/ml for 6 hours, followed by RNA harvesting. Similar experiments were performed with a more purified EPC population which was directly obtained from control bone marrow. In brief, CD133+ cells obtained from healthy control bone marrows (AllCells, | Full Paper | |
2011 | Panas, MW; | The identification and elimination of immunosuppressive Mycobacterium bovis BCG genes to create novel rBCG vectors that generate increased CD8+ T cell responses | Thesis | The delivery of an allelic exchange substrate to allcells within a mycobacterial population will be accomplished through specializedtransduction using the temperature sensitive phage Save | Full Paper | |
2011 | Hermann, RJ; | Investigation of Ikaros-dependent transcriptional regulation of novel VPAC1 splice variants during epigenetic transformation | Thesis | In order to identify which PBMC were the cell source for smaller VPAC1 isoforms, purified samples of different cell populations that compose the collective PBMC pool had to be analyzed. cDNA from purified peripheral blood cell populations was purchas | Full Paper | |
2011 | Furman, PA;Murakami, E;Niu, C;Lam, AM;Espiritu, C;Bansal, S;Bao, H;Tolstykh, T;Micolochick Steuer, H;Keilman, M;Zennou, V;Bourne, N;Veselenak, RL;Chang, W;Ross, BS;Du, J;Otto, MJ;Sofia, MJ; | Activity and the metabolic activation pathway of the potent and selective hepatitis C virus pronucleotide inhibitor PSI-353661 | Antiviral research | 21600932 | The human bone marrow stem cell toxicity assay was performed by Reachbio (Seattle, WA). Clonogenic progenitor cells of the erythroid (CFU-E and BFU-E) and myeloid (CFU-GM) Save | Full Paper |
2011 | Lam, AM;Espiritu, C;Murakami, E;Zennou, V;Bansal, S;Micolochick Steuer, HM;Niu, C;Keilman, M;Bao, H;Bourne, N;Veselenak, RL;Reddy, PG;Chang, W;Du, J;Nagarathnam, D;Sofia, MJ;Otto, MJ;Furman, PA; | Inhibition of hepatitis C virus replicon RNA synthesis by PSI-352938, a cyclic phosphate prodrug of β-D-2'-deoxy-2'-α-fluoro-2'-β-C-methylguanosine | Antimicrobial agents and chemotherapy | 21444700 | marrow (Lonza, Walkersville, MD) were performed by Reachbio (Seattle, WA) using a semisolid We also thank Emer Clarke at ReachBio LLC (Seattle, WA) for performing human bone Save | Full Paper |
2011 | Veeranki, S;Duan, X;Panchanathan, R;Liu, H;Choubey, D; | IFI16 protein mediates the anti-inflammatory actions of the type-I interferons through suppression of activation of caspase-1 by inflammasomes | PloS one | 22046441 | y the AIM2 and NLRP3 inflammasomes. Materials and Methods Cells and Treatments Frozen human peripheral blood mononuclear cells (PBMCs), purified CD14+ monocytes and CD19+ B cells were purchased from ReachBio (Seattle, WA). THP-1 cells were purchased | Full Paper |
2011 | Maier, MS; | Setting occupational exposure limits for unstudied pharmaceutical intermediates using an in vitro parallelogram approach | Toxicology mechanisms and methods | 20156007 | The mean ± 1 SD was calculated for triplicate cultures. The laboratory (ReachBio LLC, Seattle, Washington) was blinded to the identify of the test compounds and docetaxel served as the positive control, with blank and vehicle as negative controls | Full Paper |
2011 | Lautenschlager, F; | Cell compliance: cytoskeletal origin and importance for cellular function. | Thesis | Human MSCs (hMSCs) were investigated over the time they needed to double their population 17 times, which corresponds to about eight passages. Cells were obtained from the bone marrow of adult donors (Stem Cell Technologies and ReachBio LLC), isolate | Full Paper | |
2010 | Yelamanchili, SV;Chaudhuri, AD;Chen, LN;Xiong, H;Fox, HS; | MicroRNA-21 dysregulates the expression of MEF2C in neurons in monkey and human SIV/HIV neurological disease | Cell Death Dis | 21170291 | miRNA in situ hybridization. Following necropsy, monkey caudate and hippocampus were formalin fixed, and embedded in paraffin, and 5 μm sections cut onto glass slides. The in situ hybridization was performed at Phylogeny Inc. (Columbus, OH, USA) as d | Full Paper |
2010 | Maret, D;Gruzglin, E;Sadr, MS;Siu, V;Shan, W;Koch, AW;Seidah, NG;Del Maestro, RF;Colman, DR; | Surface expression of precursor N-cadherin promotes tumor cell invasion | Neoplasia | 21170270 | Melanoma, breast, squamous cell, and prostate carcinoma tissue samples and arrays, in addition to corresponding normal tissue, were obtained from FolioBio (Columbus, OH). Metastatic melanoma arrays were from Imgenex (San Diego, CA). Cell Culture and | Full Paper |
2010 | Teichert, A;Elalieh, H;Bikle, D; | Disruption of the hedgehog signaling pathway contributes to the hair follicle cycling deficiency in Vdr knockout mice | J. Cell. Physiol. | 20458748 | The binding of the antibodies was detected by biotinylated goat anti‐rabbit IgG, followed by ABC‐peroxidase reagent (Vector, Burlingame, CA) and revealed with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followed by hematoxyli | Full Paper |
2010 | Kashyap, MK;Harsha, HC;Renuse, S;Pawar, H;Sahasrabuddhe, NA;Kim, MS;Marimuthu, A;Keerthikumar, S;Muthusamy, B;Kandasamy, K;Subbannayya, Y;Prasad, TS;Mahmood, R;Chaerkady, R;Meltzer, SJ;Kumar, RV;Rustgi, AK;Pandey, A; | SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome | Cancer Biol. Ther. | 20686364 | ... _id":"553574440","term_text":"ESC96101"}}ESC96101 [/protein/ESC96101]) consisted of 34 cases in duplicate, ranging from differentiation grades I–III from patient's ages 36–71 years. The TMAs from FolioBio (catalog # ARY-HH0091) consisted of 40 ES | Full Paper |
2010 | Li, Z;Qi, CF;Shin, DM;Zingone, A;Newbery, HJ;Kovalchuk, AL;Abbott, CM;Morse, HC; | Eef1a2 promotes cell growth, inhibits apoptosis and activates JAK/STAT and AKT signaling in mouse plasmacytomas | PLoS ONE | 20505761 | correction. A tissue microarray of costal biopsies of normal individuals or patients diagnosed with MM was purchased from Folio BioSciences (Columbus, OH). Immunohistochemical studies were detailed previously [27]. Mouse | Full Paper |
2010 | Shih, DM;Xia, YR;Yu, JM;Lusis, AJ; | Temporal and tissue-specific patterns of Pon3 expression in mouse: in situ hybridization analysis | Adv. Exp. Med. Biol. | 20221872 | Detailed biochemical studies are also necessary to identify the physiological substrate(s) of PON3 in various cells/tissues. Notes. Acknowledgment. We thank Yi-Shou Shi, XuPing Wang, and Phylogeny, Inc. (Columbus, OH) for excellent technical support | Full Paper |
2010 | Hill, LS;Richardson, TL;Profeta, LT;Shaw, TJ;Hintz, CJ;Twining, BS;Lawrenz, E;Myrick, ML; | Construction, figures of merit, and testing of a single-cell fluorescence excitation spectroscopy system | Rev Sci Instrum | 20113077 | The relay box uses a Bud Industries PN series box enclosure (model PN-1341-C) as the base. The circuit consisted of two NTE Electronics solid-state relays (model RS3–1D10–51), two Omron Electronic relays (model G4B-112T-C-U.S.-AC120), one Qualtek Ele | Full Paper |
2010 | Gentles, AJ;Plevritis, SK;Majeti, R;Alizadeh, AA; | Association of a leukemic stem cell gene expression signature with clinical outcomes in acute myeloid leukemia | JAMA | 21177505 | Normal human bone marrow mononuclear cells were purchased from AllCells Inc (Emeryville, California), and human cord blood was obtained from Stanford University Medical Center. Save | Full Paper |
2010 | Carter, CC;Onafuwa-Nuga, A;McNamara, LA;Riddell, J;Bixby, D;Savona, MR;Collins, KL; | HIV-1 infects multipotent progenitor cells causing cell death and establishing latent cellular reservoirs | Nature medicine | 20208541 | minates all consensus AP-1, NF-AT, NF-κB SP-1 and TATA motifs, but maintains the polyA site found in the R region. Cell culture Fresh whole bone marrow aspirates were obtained commercially (AllCells ltd.). Pre-existing umbilical cord blood lacking p | Full Paper |
2010 | Naka, K;Hoshii, T;Muraguchi, T;Tadokoro, Y;Ooshio, T;Kondo, Y;Nakao, S;Motoyama, N;Hirao, A; | TGF-beta-FOXO signalling maintains leukaemia-initiating cells in chronic myeloid leukaemia | Nature | 20130650 | solid medium. Human LICs were purified as CD34+CD38-Lin- cells of bone marrow cells from CML patients (Cureline, Inc. and AllCells). These cells were co-cultured on OP-9 cells for 5 days before transfer to semi-solid medium. Cell cycling was assessed | Full Paper |
2010 | Lishko, PV;Botchkina, IL;Fedorenko, A;Kirichok, Y; | Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel | Cell | 20144758 | Frozen normal peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryville, CA), thawed and plated onto sterile coverslips according to the manufacturer instructions. Mouse monoclonal anti-COXIV and anti-CD14 antibody were from | Full Paper |
2010 | Loewer, S;Cabili, MN;Guttman, M;Loh, YH;Thomas, K;Park, IH;Garber, M;Curran, M;Onder, T;Agarwal, S;Manos, PD;Datta, S;Lander, ES;Schlaeger, TM;Daley, GQ;Rinn, JL; | Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells | Nature genetics | 21057500 | The CD34 + cells used were mobilized peripheral blood CD34+ cells (AllCells, mBP014F). Cells were thawed and cultured as described 21 for two days before RNA isolation. Save | Full Paper |
2010 | Pichiorri, F;Suh, SS;Rocci, A;De Luca, L;Taccioli, C;Santhanam, R;Zhou, W;Benson, DM;Hofmainster, C;Alder, H;Garofalo, M;Di Leva, G;Volinia, S;Lin, HJ;Perrotti, D;Kuehl, M;Aqeilan, RI;Palumbo, A;Croce, CM; | Downregulation of p53-inducible microRNAs 192, 194, and 215 impairs the p53/MDM2 autoregulatory loop in multiple myeloma development | Cancer cell | 20951946 | IRB-approved procurement protocol (2000C0247) at The Ohio State University and The University of Turin GIMEMA-MM-03-05, N. EUDRACT 2005-004745-33). Three of the five healthy PCs used were purchased from AllCells LLC. (Emeryville, CA) | Full Paper |
2010 | Figueroa, ME;Lugthart, S;Li, Y;Erpelinck-Verschueren, C;Deng, X;Christos, PJ;Schifano, E;Booth, J;van Putten, W;Skrabanek, L;Campagne, F;Mazumdar, M;Greally, JM;Valk, PJ;Löwenberg, B;Delwel, R;Melnick, A; | DNA methylation signatures identify biologically distinct subtypes in acute myeloid leukemia | Cancer cell | 20060365 | We hypothesized that DNA methylation distributes into specific patterns in cancer cells, which reflect critical biological differences. We therefore examined the methylation profiles of 344 Save | Full Paper |
2010 | Liu, W;Lee, HW;Liu, Y;Wang, R;Rodgers, GP; | Olfactomedin 4 is a novel target gene of retinoic acids and 5-aza-2'-deoxycytidine involved in human myeloid leukemia cell growth, differentiation, and apoptosis | Blood | 20724538 | Peripheral blood mononuclear cells from AML patients and normal individuals, and neutrophils from normal individuals, were obtained commercially from AllCells. The neutrophils were Save | Full Paper |
2010 | Tatsumi, K;Taatjes, D;Bouchard, B;Trotman, W;Bovill, E; | Cell Adhesion Molecule 1 (CADM1), a Novel Venous Thrombosis Risk Factor, Is Ubiquitously Present In Vascular Endothelium and Smooth Muscle Cells | Blood | Immunoelectron microscopy (IEM) was performed on cultured human umbilical vein endothelial cells (Allcells LLC, Emeryville, CA) using a post embedding procedure with protein-A Save | Full Paper | |
2010 | Lin, X;Rice, K;Carroll, M;Costa, F;Kilpivaara, O;Soares, M;Levine, R;Licht, J; | Mir-9 Is Aberrantly Expressed In MPN Patients and Accelerates Erythropoietic Cell Growth and Differentiation | Blood | To determine how deregulated expression of miR-9 affected erythropoiesis, we transduced human bone marrow CD34+ cells (AllCells) with empty vector or miR-9 lentivirus, and Save;Abstract 1983 The myeloproliferative neoplasms (MPNs), P | Full Paper | |
2010 | Campbell, JE;Brummel-Ziedins, KE;Butenas, S;Mann, KG; | Cellular regulation of blood coagulation: a model for venous stasis | Blood | 20864579 | We have adapted the corn-trypsin inhibitor whole-blood model to include EA.hy926 as an endothelium surrogate to evaluate the vascular modulation of blood coagulation initiated by Save | Full Paper |
2010 | Sangokoya, C;Telen, MJ;Chi, JT; | microRNA miR-144 modulates oxidative stress tolerance and associates with anemia severity in sickle cell disease | Blood | 20709907 | Normal hemoglobin (HbAA) CD34+ cells (ALLCells) and HbSS CD34+ cells were purified from blood collected from apheresis with donor consent according to Duke Institutional Review Board protocol. Mononuclear cells were separated by Ficoll-Hypaque densit | Full Paper |
2010 | Prabhala, RH;Pelluru, D;Fulciniti, M;Prabhala, HK;Nanjappa, P;Song, W;Pai, C;Amin, S;Tai, YT;Richardson, PG;Ghobrial, IM;Treon, SP;Daley, JF;Anderson, KC;Kutok, JL;Munshi, NC; | Elevated IL-17 produced by TH17 cells promotes myeloma cell growth and inhibits immune function in multiple myeloma | Blood | 20395418 | approval by the institutional review board (IRB) at Dana-Farber Cancer Institute. Healthy donor samples were obtained from the blood donor center at Children's Hospital, Boston, MA. Normal donor bone marrow samples were obtained from AllCells | Full Paper |
2010 | Liu, X;Shin, N;Koblish, HK;Yang, G;Wang, Q;Wang, K;Leffet, L;Hansbury, MJ;Thomas, B;Rupar, M;Waeltz, P;Bowman, KJ;Polam, P;Sparks, RB;Yue, EW;Li, Y;Wynn, R;Fridman, JS;Burn, TC;Combs, AP;Newton, RC;Scherle, PA; | Selective inhibition of IDO1 effectively regulates mediators of antitumor immunity | Blood | 20197554 | Bone marrow mononuclear cells of patients with AML were purchased from ALLCELLS and maintained according to the recommended culture conditions. Save | Full Paper |
2010 | Quintás-Cardama, A;Vaddi, K;Liu, P;Manshouri, T;Li, J;Scherle, PA;Caulder, E;Wen, X;Li, Y;Waeltz, P;Rupar, M;Burn, T;Lo, Y;Kelley, J;Covington, M;Shepard, S;Rodgers, JD;Haley, P;Kantarjian, H;Fridman, JS;Verstovsek, S; | Preclinical characterization of the selective JAK1/2 inhibitor INCB018424: therapeutic implications for the treatment of myeloproliferative neoplasms | Blood | 20130243 | Mononuclear cells were isolated from peripheral blood from patients with PV or normal control persons by centrifugation through Ficoll (Allcells). A total of 2 × 10 5 cells from control or Save | Full Paper |
2010 | Swift, S;Ellison, AR;Kassner, P;McCaffery, I;Rossi, J;Sinclair, AM;Begley, CG;Elliott, S; | Absence of functional EpoR expression in human tumor cell lines | Blood | 20124514 | Erythroid progenitor cells were generated from CD34 + peripheral blood cells mobilized with granulocyte colony-stimulating factor (G-CSF; ALLCELLS) cultured for 6 days in StemSpan Save | Full Paper |
2010 | Driscoll, JJ;Pelluru, D;Lefkimmiatis, K;Fulciniti, M;Prabhala, RH;Greipp, PR;Barlogie, B;Tai, YT;Anderson, KC;Shaughnessy, JD;Annunziata, CM;Munshi, NC; | The sumoylation pathway is dysregulated in multiple myeloma and is associated with adverse patient outcome | Blood | 19965618 | Fresh, normal, human adult bone marrow was from AllCells, and CD138 + cells were affinity purified by positive selection using Miltenyi CD138 + microbeads (Miltenyi Biotec) according Save | Full Paper |
2010 | Lee, CH;Shah, B;Moioli, EK;Mao, JJ; | CTGF directs fibroblast differentiation from human mesenchymal stem/stromal cells and defines connective tissue healing in a rodent injury model | The Journal of clinical investigation | 20679726 | Human MSCs were isolated from fresh whole bone marrow samples of 2 anonymous adult donors (age range, 20-25 years; AllCells) and were exempt from IRB approval. Nonhematopoietic, mononucleated and adherent cells were purified by centrifugation through | Full Paper |
2010 | Chao, MP;Jaiswal, S;Weissman-Tsukamoto, R;Alizadeh, AA;Gentles, AJ;Volkmer, J;Weiskopf, K;Willingham, SB;Raveh, T;Park, CY;Majeti, R;Weissman, IL; | Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47 | Science translational medicine | 21178137 | Normal human bone marrow mononuclear cells were purchased from AllCells Inc. Normal peripheral blood and human cancer samples were obtained from patients at the Stanford Save | Full Paper |
2010 | Collins, JM;Ayala, P;Desai, TA;Russell, B; | Three-dimensional culture with stiff microstructures increases proliferation and slows osteogenic differentiation of human mesenchymal stem cells | Small (Weinheim an der Bergstrasse, Germany) | 19943257 | however, experiments were performed on P2 hMSCs obtained from 2 separate donors. Additionally, some experiments were repeated with P2 hMSCs isolated from whole marrow (AllCells, Berkely, CA) as described previously.[29] | Full Paper |
2010 | Cheng, S;Gao, N;Zhang, Z;Chen, G;Budhraja, A;Ke, Z;Son, YO;Wang, X;Luo, J;Shi, X; | Quercetin induces tumor-selective apoptosis through downregulation of Mcl-1 and activation of Bax | Clinical cancer research : an official journal of the American Association for Cancer Research | 21138867 | Mononuclear cells were isolated from peripheral blood or bone marrow of leukemia patients or healthy donors were purchased from AllCells, LLC. Mononuclear cells were suspended Save | Full Paper |
2010 | Berkofsky-Fessler, W;Buzzai, M;Kim, MK;Fruchtman, S;Najfeld, V;Min, DJ;Costa, FF;Bischof, JM;Soares, MB;McConnell, MJ;Zhang, W;Levine, R;Gilliland, DG;Calogero, R;Licht, JD; | Transcriptional profiling of polycythemia vera identifies gene expression patterns both dependent and independent from the action of JAK2V617F | Clinical cancer research : an official journal of the American Association for Cancer Research | 20601445 | Normal bone marrow CD34 + cells were purchased from Allcells. To isolate CD34 + cells, bone marrow samples were diluted 1:1 in PBS and layered onto Histopaque 1077 (Sigma; Save | Full Paper |
2010 | Marchi, E;Paoluzzi, L;Scotto, L;Seshan, VE;Zain, JM;Zinzani, PL;O'Connor, OA; | Pralatrexate is synergistic with the proteasome inhibitor bortezomib in in vitro and in vivo models of T-cell lymphoid malignancies | Clinical cancer research : an official journal of the American Association for Cancer Research | 20501616 | Peripheral blood mononuclear cells (PBMC) from healthy donors were purchased from Allcells. All cell lines were grown as previously described (21). Save | Full Paper |
2010 | Paoluzzi, L;Scotto, L;Marchi, E;Zain, J;Seshan, VE;O'Connor, OA; | Romidepsin and belinostat synergize the antineoplastic effect of bortezomib in mantle cell lymphoma | Clinical cancer research : an official journal of the American Association for Cancer Research | 20068080 | Mononuclear cells from PBMC samples of healthy donors were purchased from Allcells. All cell lines were grown as described previously (12, 13). Save | Full Paper |
2010 | Brunckhorst, MK;Wang, H;Lu, R;Yu, Q; | Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis | Cancer research | 20823154 | Human umbilical vein ECs (HUVEC) and normal human astrocytes (NHA) were from AllCells, Inc., U87MG was obtained from American Type Culture Collection, and U251 was Save | Full Paper |
2010 | Cho, SY;Xu, M;Roboz, J;Lu, M;Mascarenhas, J;Hoffman, R; | The effect of CXCL12 processing on CD34+ cell migration in myeloproliferative neoplasms | Cancer research | 20388788 | Fresh normal marrow was purchased from AllCells LLC. Cord blood (CB) collections were provided by Cord Blood Bank of New York Blood Center. Save | Full Paper |
2010 | Xu, Y;Stamenkovic, I;Yu, Q; | CD44 attenuates activation of the hippo signaling pathway and is a prime therapeutic target for glioblastoma | Cancer research | 20197461 | C, expression of endogenous CD44 in a panel of human glioma cells and NHAs (ALLCELLS, Inc.) was determined by Western blotting using anti-CD44 antibody (Santa Cruz). Total Save | Full Paper |
2010 | Hart, S;Goh, K;Tan, Y;Amalini, C;Wood, J; | Abstract 2524: SB1518, a novel oral JAK2-FLT3 inhibitor, is efficacious in models of acute myeloid leukemia and primary leukemic cells from patients | Cancer Research | For progenitor expansion, PBMCs of AML patients (AllCells Inc) were cultured using a medium containing IL-3, IL-6, SCF and FLT3L (StemCell Technologies TB 28440). The expanded Save | Full Paper | |
2010 | Santanam, U;Zanesi, N;Efanov, A;Costinean, S;Palamarchuk, A;Hagan, JP;Volinia, S;Alder, H;Rassenti, L;Kipps, T;Croce, CM;Pekarsky, Y; | Chronic lymphocytic leukemia modeled in mouse by targeted miR-29 expression | Proceedings of the National Academy of Sciences of the United States of America | 20566844 | Real-time PCR experiments were carried out using miR-29a, miR-29b, and PXDN assays for real-time PCR (Applied Biosystems) according to the manufacturer's protocol. Control human cord blood CD19+ B cells were purchased from Allcells and Lonza | Full Paper |
2010 | Mi, S;Li, Z;Chen, P;He, C;Cao, D;Elkahloun, A;Lu, J;Pelloso, LA;Wunderlich, M;Huang, H;Luo, RT;Sun, M;He, M;Neilly, MB;Zeleznik-Le, NJ;Thirman, MJ;Mulloy, JC;Liu, PP;Rowley, JD;Chen, J; | Aberrant overexpression and function of the miR-17-92 cluster in MLL-rearranged acute leukemia | Proceedings of the National Academy of Sciences of the United States of America | 20133587 | obtained at the time of diagnosis or relapse and with informed consent at the University of Chicago or other hospitals (see characteristics of patients in Table S2). Normal control cells were purchased from AllCells. (Emeryville, CA) | Full Paper |
2010 | Wu, PK;Ringeisen, BR; | Development of human umbilical vein endothelial cell (HUVEC) and human umbilical vein smooth muscle cell (HUVSMC) branch/stem structures on hydrogel layers via biological laser printing (BioLP) | Biofabrication | 20811126 | HUVEC were purchased from Allcells, and HUVSMC were purchased from ATCC. Matrigel was purchased from BD Bioscience. The cells were grown in a humidified environment with 5 Save | Full Paper |
2010 | Bhatwadekar, AD;Guerin, EP;Jarajapu, YP;Caballero, S;Sheridan, C;Kent, D;Kennedy, L;Lansang, MC;Ruscetti, FW;Pepine, CJ;Higgins, PJ;Bartelmez, SH;Grant, MB; | Transient inhibition of transforming growth factor-beta1 in human diabetic CD34+ cells enhances vascular reparative functions | Diabetes | 20460428 | CD34 + cells enriched by Miltenyi positive selection from human cord blood and bone marrow obtained from AllCells (Emeryville, CA) and were >95% pure as assessed by fluorescence Save | Full Paper |
2010 | Lin, TH;Hegen, M;Quadros, E;Nickerson-Nutter, CL;Appell, KC;Cole, AG;Shao, Y;Tam, S;Ohlmeyer, M;Wang, B;Goodwin, DG;Kimble, EF;Quintero, J;Gao, M;Symanowicz, P;Wrocklage, C;Lussier, J;Schelling, SH;Hewet, AG;Xuan, D;Krykbaev, R;Togias, J;Xu, X;Harrison, R;Mansour, T;Collins, M;Clark, JD;Webb, ML;Seidl, KJ; | Selective functional inhibition of JAK-3 is sufficient for efficacy in collagen-induced arthritis in mice | Arthritis and rheumatism | 20506481 | Cryopreserved peripheral blood mononuclear cells (PBMCs; AllCells) were cultured in complete RPMI 1640 medium (supplemented with 10% fetal bovine serum, penicillin/streptomycin, 2 mM L-glutamine, and 1 mM sodium pyruvate) plus 10 μg/ml lectin phytohe | Full Paper |
2010 | Kodippili, GC;Spector, J;Kang, GE;Liu, H;Wickrema, A;Ritchie, K;Low, PS; | Analysis of the kinetics of band 3 diffusion in human erythroblasts during assembly of the erythrocyte membrane skeleton | British journal of haematology | 20553270 | Baxter Healthcare, Inc., Deerfield, IL) from commercially available growth factor mobilized blood samples (AllCells LLC, Emeryville, CA), as described elsewhere (Kang, et al 2008). Briefly, CD34+ cells isolated from growth-factor mobilized peripheral | Full Paper |
2010 | Lavelle, D;Saunthararajah, Y;Vaitkus, K;Singh, M;Banzon, V;Phiasivongsva, P;Redkar, S;Kanekal, S;Bearss, D;Shi, C;Inloes, R;DeSimone, J; | S110, a novel decitabine dinucleotide, increases fetal hemoglobin levels in baboons (P. anubis) | Journal of translational medicine | 20932295 | Frozen CD34+ human cells purified from the peripheral blood of mobilized donors were purchased from Allcells, Inc. These cells were cultured in Iscove's media containing 20% fetal Save | Full Paper |
2010 | Yamaza, T;Kentaro, A;Chen, C;Liu, Y;Shi, Y;Gronthos, S;Wang, S;Shi, S; | Immunomodulatory properties of stem cells from human exfoliated deciduous teeth | Stem cell research & therapy | 20504286 | Healthy bone marrow aspirates from iliac bone and peripheral blood mononuclear cells (PBMNCs) of healthy volunteers were purchased from AllCells (Berkeley, CA, USA). Save | Full Paper |
2010 | Luo, XM;Lei, MY;Feidi, RA;West, AP;Balazs, AB;Bjorkman, PJ;Yang, L;Baltimore, D; | Dimeric 2G12 as a potent protection against HIV-1 | PLoS pathogens | 21187894 | ting amounts of monomer or dimer from the separated purified species. Humanized mice, passive transfer, and HIV-1 challenge Frozen human cord blood CD34+ cells from single donors were purchased from AllCells (Emeryville, CA) or Lonza (Basel, Switzer | Full Paper |
2010 | Ellis-Connell, AL;Iempridee, T;Xu, I;Mertz, JE; | Cellular microRNAs 200b and 429 regulate the Epstein-Barr virus switch between latency and lytic replication | Journal of virology | 20668090 | Raji cell assay as described above. Approximately 1 × 105 GRUs/ml was used to infect 1 × 106 cells/ml of primary blood B cells (AllCells, Emeryville, CA). Samples were harvested at the times indicated in Fig. 2 and processed as described above for ZE | Full Paper |
2010 | Li, J;Favata, M;Kelley, JA;Caulder, E;Thomas, B;Wen, X;Sparks, RB;Arvanitis, A;Rogers, JD;Combs, AP;Vaddi, K;Solomon, KA;Scherle, PA;Newton, R;Fridman, JS; | INCB16562, a JAK1/2 selective inhibitor, is efficacious against multiple myeloma cells and reverses the protective effects of cytokine and stromal cell support | Neoplasia (New York, N.Y.) | 20072651 | Primary bone marrow CD138+ plasma cells from a newly diagnosed MM patient were purchased from Allcells (Emeryville, CA). The cells were cultured in the same medium used for Save | Full Paper |
2010 | Jekle, A;Chhabra, M;Lochner, A;Meier, S;Chow, E;Brandt, M;Sankuratri, S;Cammack, N;Heilek, G; | Epitope switching as a novel escape mechanism of HIV to CCR5 monoclonal antibodies | Antimicrobial agents and chemotherapy | 19995923 | 10% FBS and 1% penicillin-streptomycin. Human peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA), stimulated for 1 day in PBMC medium (RPMI 1640 medium containing 10% FBS, 1% penicillin-streptomycin, 2 mM l-glutami | Full Paper |
2010 | Culpin, RE;Proctor, SJ;Angus, B;Crosier, S;Anderson, JJ;Mainou-Fowler, T; | A 9 series microRNA signature differentiates between germinal centre and activated B-cell-like diffuse large B-cell lymphoma cell lines | International journal of oncology | 20596664 | streptomycin (Gibco), according to the repositories guidelines. Cultures were incubated at 37˚C in a 5% CO2 humidified atmosphere. CD19+ negatively-selected B-cell total RNA (pooled from 3 unrelated donors) served as the normal healthy B-cell control | Full Paper |
2010 | Collins, JM;Goldspink, PH;Russell, B; | Migration and proliferation of human mesenchymal stem cells is stimulated by different regions of the mechano-growth factor prohormone | Journal of molecular and cellular cardiology | 20875825 | 2 mM L-glutamine, 100 units/mL penicillin and 100 μg/mL streptomycin. hMSCs were obtained from the Tulane University Center for Gene Therapy or AllCells (Berkeley, CA). Passage 2-3 (P2-P3) hMSCs were used for all experiments | Full Paper |
2010 | Destache, CJ;Belgum, T;Goede, M;Shibata, A;Belshan, MA; | Antiretroviral release from poly(DL-lactide-co-glycolide) nanoparticles in mice | The Journal of antimicrobial chemotherapy | 20729545 | Frozen elutriated monocytes were obtained from AllCells, LLC (Emeryville, CA, USA). Cells were thawed in a water bath at 37C and counted using Trypan Blue exclusion. Cells (5 × 10 Save | Full Paper |
2010 | Berahovich, RD;Zabel, BA;Penfold, ME;Lewén, S;Wang, Y;Miao, Z;Gan, L;Pereda, J;Dias, J;Slukvin, II;McGrath, KE;Jaen, JC;Schall, TJ; | CXCR7 protein is not expressed on human or mouse leukocytes | Journal of immunology (Baltimore, Md. : 1950) | 20889540 | Human normal bone marrow cells were purchased from AllCells (Emeryville, CA). Human thymocytes were obtained by mechanical disruption of fetal thymus tissue purchased from Save | Full Paper |
2010 | Thacker, SG;Berthier, CC;Mattinzoli, D;Rastaldi, MP;Kretzler, M;Kaplan, MJ; | The detrimental effects of IFN-α on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction | Journal of immunology (Baltimore, Md. : 1950) | 20805419 | Similar experiments were performed with EPCs directly obtained from control bone marrow. In brief, CD133+ cells obtained from healthy control bone marrows (AllCells, Emeryville, CA) were thawed following manufacturer’s recommendations and then cultur | Full Paper |
2010 | Toti, US;Guru, BR;Grill, AE;Panyam, J; | Interfacial activity assisted surface functionalization: a novel approach to incorporate maleimide functional groups and cRGD peptide on polymeric nanoparticles for targeted drug delivery | Molecular pharmaceutics | 20527782 | methyl tri-chlorosilane were from Sigma-Aldrich (St Louis, MO). RPMI cell media, fetal bovine serum, and trypsin were purchased from Invitrogen (Carlsbad, CA). HUVEC media was supplied by AllCells (Emeryville, CA) | Full Paper |
2010 | Medina, RJ;O'Neill, CL;Humphreys, MW;Gardiner, TA;Stitt, AW; | Outgrowth endothelial cells: characterization and their potential for reversing ischemic retinopathy | Investigative ophthalmology & visual science | 20554606 | of 1 × 107 cells/mL. Population doubling was carefully monitored, and OECs at early passages were used for all experiments. Frozen human cord blood mononuclear cells were purchased (Allcells; Stem Cell Technologies, Emeryville, CA) and plated under t | Full Paper |
2010 | Kopher, RA;Penchev, VR;Islam, MS;Hill, KL;Khosla, S;Kaufman, DS; | Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells | Bone | 20601304 | Mayo Institutional Review Board and obtaining informed consent, or isolated from whole bone marrow (AllCells, Emeryville, CA, USA) using standard methods [50]. BM-MSCs were cultured in MSC media as described above. Adherent cells were selected for du | Full Paper |
2010 | Vanella, L;Kim, DH;Asprinio, D;Peterson, SJ;Barbagallo, I;Vanella, A;Goldstein, D;Ikehara, S;Kappas, A;Abraham, NG; | HO-1 expression increases mesenchymal stem cell-derived osteoblasts but decreases adipocyte lineage | Bone | 19853072 | Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential Save | Full Paper |
2010 | Kim, DH;Vanella, L;Inoue, K;Burgess, A;Gotlinger, K;Manthati, VL;Koduru, SR;Zeldin, DC;Falck, JR;Schwartzman, ML;Abraham, NG; | Epoxyeicosatrienoic acid agonist regulates human mesenchymal stem cell-derived adipocytes through activation of HO-1-pAKT signaling and a decrease in PPARγ | Stem cells and development | 20412023 | Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α Save | Full Paper |
2010 | Fox, LE;Shen, J;Ma, K;Liu, Q;Shi, G;Pappas, GD;Qu, T;Cheng, J; | Membrane properties of neuron-like cells generated from adult human bone-marrow-derived mesenchymal stem cells | Stem cells and development | 20394468 | The hMeSCs from 4 donors were commercially obtained from AllCells LLC and Cambrex Bioscience. The cell lines were negative for surface markers associated with hematopoietic Save | Full Paper |
2010 | Lai, Y;Sun, Y;Skinner, CM;Son, EL;Lu, Z;Tuan, RS;Jilka, RL;Ling, J;Chen, XD; | Reconstitution of marrow-derived extracellular matrix ex vivo: a robust culture system for expanding large-scale highly functional human mesenchymal stem cells | Stem cells and development | 19737070 | Freshly isolated human bone marrow mononuclear cells obtained from 20- to 30-year-old donors were purchased from ALLCELLS (Emeryville, CA), and grown on tissue culture plastic Save | Full Paper |
2010 | McLaughlin, J;Markovtsov, V;Li, H;Wong, S;Gelman, M;Zhu, Y;Franci, C;Lang, D;Pali, E;Lasaga, J;Low, C;Zhao, F;Chang, B;Gururaja, TL;Xu, W;Baluom, M;Sweeny, D;Carroll, D;Sran, A;Thota, S;Parmer, M;Romane, A;Clemens, G;Grossbard, E;Qu, K;Jenkins, Y;Kinoshita, T;Taylor, V;Holland, SJ;Argade, A;Singh, R;Pine, P;Payan, DG;Hitoshi, Y; | Preclinical characterization of Aurora kinase inhibitor R763/AS703569 identified through an image-based phenotypic screen | Journal of cancer research and clinical oncology | 19609559 | Cultured human primary mast cells were established from human umbilical cord blood CD34 + progenitor cells (AllCells, LLC, Berkeley, CA) using the method described in US patent no Save | Full Paper |
2010 | Chen, NK;Tan, SY;Udolph, G;Kon, OL; | Insulin expressed from endogenously active glucose-responsive EGR1 promoter in bone marrow mesenchymal stromal cells as diabetes therapy | Gene therapy | 20182520 | HBMMSC (AllCells, Emeryville, CA, USA) cultured overnight in Dulbecco's modified Eagle's medium (DMEM)-3 mM glucose (as the low glucose baseline) were exposed to DMEM-20 mM glucose (high glucose) for 15 or 30 min, and then returned to DMEM-3 mM gluco | Full Paper |
2010 | Marchi, E; | Pralatrexate sinergizza in vitro ed in vivo con l'inibitore del Proteosoma Bortezomib in modelli pre-clinici di Linfoma Non Hodkin T | Thesis | aggiunto a giorni alterni. Le cellule sono state mantenute in coltura alla concentrazione ideale di 0.5 x 106 cellule/mL e trattate come descritto in precedenti studi pubblicati52 . Le cellule mononucleate del sangue periferico da donatori volontari | Full Paper | |
2010 | Qureshi, A;Subathra, M;Grey, A;Schey, K;Del Poeta, M;Luberto, C; | Role of sphingomyelin synthase in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformans | PloS one | 21203393 | Approximately 6.4×10 4 fresh human normal peripheral blood-neutrophils per ml (AllCells) were incubated in 124 µl sterile filtered PBS supplemented with 10% pooled human serum Save | Full Paper |
2010 | O'Connell, RM;Balazs, AB;Rao, DS;Kivork, C;Yang, L;Baltimore, D; | Lentiviral vector delivery of human interleukin-7 (hIL-7) to human immune system (HIS) mice expands T lymphocyte populations | PloS one | 20700454 | 2×10 7 PBMCs (AllCells) that were first labeled with CFSE. HIS mice were created as described[5]. In brief, 1.5×10 5 highly purified CD34+ CB stem cells (AllCells) were injected into the Save | Full Paper |
2010 | Smith, B;Treadwell, J;Zhang, D;Ly, D;McKinnell, I;Walker, PR;Sikorska, M; | Large-scale expression analysis reveals distinct microRNA profiles at different stages of human neurodevelopment | PloS one | 20559549 | obtained from ScienCell Research Laboratories (Carlsbad, CA) and were directly pelleted for RNA extraction. Frozen primary human fetal astrocytes (PHAf) from 20-21 week fetuses were received from ALLCELLS (Emeryville, CA) and were cultured in Astroc | Full Paper |
2010 | Kachlany, SC;Schwartz, AB;Balashova, NV;Hioe, CE;Tuen, M;Le, A;Kaur, M;Mei, Y;Rao, J; | Anti-leukemia activity of a bacterial toxin with natural specificity for LFA-1 on white blood cells | Leukemia research | 19747730 | The oral bacterium, Aggregatibacter actinomycetemcomitans, produces a leukotoxin (LtxA) that is specific for white blood cells (WBCs) from humans and Old World primates by Save | Full Paper |
2010 | Medina, RJ;O'Neill, CL;Sweeney, M;Guduric-Fuchs, J;Gardiner, TA;Simpson, DA;Stitt, AW; | Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities | BMC medical genomics | 20465783 | l approval from three female volunteer subjects aged 25-35, non-smokers, not receiving any medication and without any clinical diagnosis; while CB-derived mononuclear cells (MNCs) were purchased from AllCells LLC (California, USA). MNCs were isolated | Full Paper |
2010 | Rowe, DD;Leonardo, CC;Hall, AA;Shahaduzzaman, MD;Collier, LA;Willing, AE;Pennypacker, KR; | Cord blood administration induces oligodendrocyte survival through alterations in gene expression | Brain research | 20883670 | Oligodendrocytes (OLs), the predominant cell type found in cerebral white matter, are essential for structural integrity and proper neural signaling. Very little is known concerning stroke- Save | Full Paper |
2010 | Jin, Q;Alkhatib, B;Cornetta, K;Alkhatib, G; | Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1 | Virology | 19913864 | streptomycin sulfate (0.1 mg/ml) in a humidified incubator held at 5% CO2. All cell culture media were purchased from GIBCO. Normal Human Astrocytes (NHA) and their basal medium (with 10% FBS) were purchased from AllCells (AllCells, LLC., Emeryville, | Full Paper |
2010 | Walczak-Drzewiecka, A;Ratajewski, M;Pułaski, Ł;Dastych, J; | DNA methylation-dependent suppression of HIF1A in an immature hematopoietic cell line HMC-1 | Biochemical and biophysical research communications | 19995553 | cDNAs from BM CD34+, PB CD34+, BM MNCs, PB MNCs and BM MSCs purchased from AllCells (Emeryville, CA, USA) was obtained from a population of positive cells (purity exceeding 95%) collected from five individual donors. The level of cognate cDNA was mea | Full Paper |
2010 | Li, W;Hong, L;Hu, L;Magin, RL; | Magnetization transfer imaging provides a quantitative measure of chondrogenic differentiation and tissue development | Tissue engineering. Part C, Methods | 20373975 | Mesenchymal stem cells (MSCs), isolated from fresh adult human bone marrow obtained from AllCells LLC (Berkeley, CA), were plated at a density of 107 nucleated cells per 100-mm-diameter Petri dish. The cells were incubated in a basic medium of Dulbec | Full Paper |
2010 | Akers, SM;O'Leary, HA;Minnear, FL;Craig, MD;Vos, JA;Coad, JE;Gibson, LF; | VE-cadherin and PECAM-1 enhance ALL migration across brain microvascular endothelial cell monolayers | Experimental hematology | 20470859 | Positively selected peripheral blood CD19 + B cells from a healthy donor were purchased from AllCells (Emeryville, CA, USA). HBMEC were obtained from Angioproteomie (Boston, MA Save | Full Paper |
2010 | Schuhmann, NK;Pozzoli, O;Sallach, J;Huber, A;Avitabile, D;Perabo, L;Rappl, G;Capogrossi, MC;Hallek, M;Pesce, M;Büning, H; | Gene transfer into human cord blood-derived CD34(+) cells by adeno-associated viral vectors | Experimental hematology | 20447441 | After thawing, hCD34 + cells (AllCells) were plated and expanded for 3 days as described. Cells were then incubated with rAAV2sc (at a GOI of 1 × 10 4 ) for 18 hours, followed by an Save | Full Paper |
2010 | Lu, M;Zhang, W;Li, Y;Berenzon, D;Wang, X;Wang, J;Mascarenhas, J;Xu, M;Hoffman, R; | Interferon-alpha targets JAK2V617F-positive hematopoietic progenitor cells and acts through the p38 MAPK pathway | Experimental hematology | 20303384 | Objective Interferon-α (IFNα) therapy leads to hematological remissions and a reduction of the JAK2V617F allele burden in patients with polycythemia vera (PV). In this study, the Save | Full Paper |
2010 | Yourek, G;McCormick, SM;Mao, JJ;Reilly, GC; | Shear stress induces osteogenic differentiation of human mesenchymal stem cells | Regenerative medicine | 20868327 | Isolated bone marrow (AllCells, Berkeley, CA, USA) from multiple healthy human donors aged between 20 and 26 years (seven male and one female) was enriched for hMSCs using RosetteSep® (StemCell Technologies, Vancouver, BC, Canada). Bone marrow was in | Full Paper |
2010 | Barbagallo, I;Vanella, A;Peterson, SJ;Kim, DH;Tibullo, D;Giallongo, C;Vanella, L;Parrinello, N;Palumbo, GA;Di Raimondo, F;Abraham, NG;Asprinio, D; | Overexpression of heme oxygenase-1 increases human osteoblast stem cell differentiation | Journal of bone and mineral metabolism | 19924377 | Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA, USA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential Save | Full Paper |
2010 | Covey, TM;Putta, S;Cesano, A; | Single cell network profiling (SCNP): mapping drug and target interactions | Assay and drug development technologies | 20158439 | Cryopreserved, Ficoll fractionated, bone marrow samples from healthy individual were purchased from AllCells (Emeryville, CA). Cells from cryogenic storage were thawed in a 37C Save | Full Paper |
2010 | Bingaman, MG;Basu, GD;Golding, TC;Chong, SK;Lassen, AJ;Kindt, TJ;Lipinski, CA; | The autophilic anti-CD20 antibody DXL625 displays enhanced potency due to lipid raft-dependent induction of apoptosis | Anti-cancer drugs | 20216307 | blood lymphocytes (PBLs) were purified from healthy donor samples using standard Ficoll-Plaque separation. Normal human peripheral blood mononuclear cells (PBMCs) were from AllCells (Emeryville, California, USA). CD56-immunomagnetic separation beads | Full Paper |
2010 | Elliott, S;Busse, L;McCaffery, I;Rossi, J;Sinclair, A;Spahr, C;Swift, S;Begley, CG; | Identification of a sensitive anti-erythropoietin receptor monoclonal antibody allows detection of low levels of EpoR in cells | Journal of immunological methods | 19887071 | Human EpoR expressing erythroid primary cells were derived from human mobilized peripheral blood CD34+ cells (AllCells, LLC, Emeryville, CA) by culture for 6 days in StemSpan SFEM media (STEMCELLS Tech; Vancouver, BC, Canada) with 10 ng/ml IL-3, 10 n | Full Paper |
2010 | Kim, ES;Kim, JJ;Park, EJ; | Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft material | The journal of advanced prosthodontics | 21165181 | Commercially available human apheresed platelet plasmas were purchased from AllCells (Emeryville, CA, USA). Donors were restricted to males under 30 years-old for quality control. Save | Full Paper |
2010 | Shytle, DR;Tan, J;Ehrhart, J;Smith, AJ;Sanberg, CD;Sanberg, PR;Anderson, J;Bickford, PC; | Effects of blue-green algae extracts on the proliferation of human adult stem cells in vitro: a preliminary study | Medical science monitor : international medical journal of experimental and clinical research | 20037479 | For cell proliferation analysis, human bone marrow cells or human CD34+ cells (AllCells, Inc.) were cultured in 96 well plates (5×10 4 /well) containing 100 µL of complete medium (RPMI 1640 medium supplemented with 5% FCS). These cells were treated f | Full Paper |
2010 | Kawauchi, S;Terasaki, H;Katano, M;Murase, J;Masuda, Y;Tamura, T;Shimadzu, M; | Quality control and monitoring for the isolation process of mesenchymal stem cells and their differentiation into osteoblasts | Genetic testing and molecular biomarkers | 20187761 | Bone marrow samples (ABM001; AllCells, Emeryville, CA) were purchased after gaining approval of the ethical 270committee of the Olympus Corporation. Table 1 shows the ages and sexes of the donors. Skin-derived fibroblasts were also purchased from Lon | Full Paper |
2010 | Abbott, C;Huang, G;Ellison, AR;Chen, C;Arora, T;Szilvassy, SJ;Wei, P; | Mouse monoclonal antibodies against human c-Mpl and characterization for flow cytometry applications | Hybridoma (2005) | 20443702 | Primary human CD34 + cells isolated from normal bone marrow or G-CSF-mobilized peripheral blood were purchased from AllCells (Emeryville, CA). Cryopreserved cells were thawed Save | Full Paper |
2010 | Clarkson-Jones, J;Page, C;Sarda, S;Swaisland, H; | 417 Human biotransformation of olaparib (AZD2281) an oral poly(ADP-ribose) polymerase (PARP) inhibitor | European Journal of Cancer Supplements | Methods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommended by the manufacturer (StemCell Technologies). Effects of SB | Full Paper | |
2010 | Nokihara, H;Yamamoto, N;Yamada, Y;Asahina, H;Shibata, T;Seki, Y;Tamura, Y;Honda, K;Misawa, S;Tamura, T; | 415 A phase I study evaluating the safety profile and pharmacokinetics of CS-1008 (Tigatuzumab), humanized monoclonal antibody targeting death receptor 5 (DR5), in Japanese patients with advanced solid tumours | European Journal of Cancer Supplements | Methods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommended by the manufacturer (StemCell Technologies). Effects of SB | Full Paper | |
2010 | Padgett, C;Lim, J;Marschke, R;Northfelt, D;Andreopoulou, E;Hoff, D;Anderes, K;Ryckman, D;Chen, T;O'Brien, S; | 414 Clinical pharmacokinetics and pharmacodynamics of CX-4945, a novel inhibitor of protein kinase CK2: Interim report from the phase 1 clinical trial | European Journal of Cancer Supplements | that is currently in phase Ib clinical trials in AML and MDS. Methods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommen | Full Paper | |
2010 | Antognoli, A; | Rag2-/-; gammac-/-immunodeficient mice, a new preclinical model to study antitumor approaches | Thesis | CD133+ human cord blood progenitor cells (derived from single donors) purchased from ALLCELLS (Emerville, CA), or kindly provided by Professor Lemoli of the Department of Hematology and Oncologic Sciences “L. e A. Seragnoli”, University of Bologna | Full Paper | |
2010 | Tanavde, V; | Commercialization of Cord Blood | Umbilical Cord Blood | AllCells, a California-based company and Lonza are leading players in this area. BioE also sells its MLPC cell line for research and is developing the use of MLPC as a platform for Save | Full Paper | |
2010 | Nowakiewicz, M; | MST-based method for 6DOF rigid body motion planning in narrow passages | 2010 IEEE/RSJ International Conference on Intelligent Robots and Systems | construction of MST give priority to cells at levels equal or less than { by always treating their weights as lesser than weights of the other cells. 3) ALLCELLS() - Return the set of all leaf cells. 4) NEIGHBORHOOD(z) - Return the set of all cells i | Full Paper | |
2010 | Briercheck, E;Freud, A;Caligiuri, M; | Human natural killer cell development | Natural Killer Cells | Fresh human BM was donated or purchased from AllCells, LLC (Berkeley, CA) and received within 24 hours of harvest. PB leukopaks were obtained from the American Red Cross. BM Save | Full Paper | |
2010 | Ringeisen, B;Othon, C;Wu, X;Krizman, D;Darfler, M;Anders, J;Wu, P; | Biological Laser Printing (BioLP) for High Resolution Cell Deposition | Cell and Organ Printing | HUVEC were purchased from Allcells, LLC (Emeryville, California) and cultured according to the protocol provided. HUSMC were purchased from ATCC and cultured according to the protocol provided by the supplier. In both cases, per ml of media is fortif | Full Paper | |
2010 | Leskov, I; | Modeling hematologic malignancies and their treatment in humanized mice | Thesis | Purified CD133* human umbilical cord hematopoietic stem cells (HSCs) were purchased from AllCells or from the National Disease Research Interchange (NDRI). Alternatively, CD133* cells were purified from fresh umbilical cord blood purchased from NDRI, | Full Paper | |
2010 | Chao, MP; | Modulating Phagocytosis: Therapeutic Targeting of the Anti-Phagocytic Signal CD47 in Human Malignancies | Thesis | Human Samples Normal human bone marrow mononuclear cells were purchased from AllCells Inc. (Emeryville, CA, USA). Human AML samples (Figure 1B) were obtained from patients at the Stanford Medical Center with informed consent, according to an IRB-appr | Full Paper | |
2010 | Lee, CH; | Connective tissue growth factor (CCN2/CTGF)-induced fibrogenesis: Biology, bioengineering and therapeutics | Thesis | Human mesenchymal stem cells (hMSCs) were isolated from commercially available fresh bone marrow samples of anonymous adult donors (AllCells, Berkeley, CA). Purification of hMSCs was accomplished by centrifugation through a density gradient (Ficoll-P | Full Paper | |
2010 | Collins, JM; | Stem cells are affected by physical and chemical components of the microenvironmental niche | Thesis | xperiments were performed on P2 hMSCs obtained from 2 separate donors. Additionally, some experiments were repeated with P2 hMSCs isolated from whole marrow (AllCells, Berkely, CA) as described previously (Alhadlaq et al., 2005) | Full Paper | |
2010 | Scaife, MD; | Truncated Cell Surface Markers Fused with Mutant Human Tmpk: Versatile Cell Fate Control Safety Cassettes for Lentiviral Vector Mediated Correction of Fabry Disease | Thesis | Human CD34+ cells were purchased from AllCells and cultured in Stem Span SFEM expansion medium (Stem Cell Technologies) and supplemented with 10 ng/ml human interleukin-6 (IL-6), 100 ng/ml human Flt3 Ligand (Flt3-L), 100 ng/ml human thrombopoietin (T | Full Paper | |
2010 | Van Craenenbroeck, E; | Exercise in chronic heart failure focus on endothelial repair | Thesis | typesmost likely have important contributions to the initiation, continuation and regulation ofvascular homeostasis In the following, the term EPC is used for general description of allcells Save | Full Paper | |
2010 | Jangam, SR; | A PCR based HIV-1 test for point-of-care application | Thesis | It was observed that there was a saturation effect being observed at total WBC 500,000 cells. To further confirm this observation, leukocyte depleted blood was spiked with different amounts of PBMC (ALLCELLS, Emeryville, CA) in the range of 46,000-2 | Full Paper | |
2010 | Sofia, MJ;Bao, D;Chang, W;Du, J;Nagarathnam, D;Rachakonda, S;Reddy, PG;Ross, BS;Wang, P;Zhang, HR;Bansal, S;Espiritu, C;Keilman, M;Lam, AM;Steuer, HM;Niu, C;Otto, MJ;Furman, PA; | Discovery of a β-d-2'-deoxy-2'-α-fluoro-2'-β-C-methyluridine nucleotide prodrug (PSI-7977) for the treatment of hepatitis C virus | Journal of medicinal chemistry | 20845908 | We acknowledge the following individuals and organizations for their contributions to the work described in this manuscript: Wuxi AppTec; ReachBio LLC; BASi; and Dr. Patrick Carroll, University of Pennsylvania | Full Paper |
2010 | Lam, AM;Murakami, E;Espiritu, C;Steuer, HM;Niu, C;Keilman, M;Bao, H;Zennou, V;Bourne, N;Julander, JG;Morrey, JD;Smee, DF;Frick, DN;Heck, JA;Wang, P;Nagarathnam, D;Ross, BS;Sofia, MJ;Otto, MJ;Furman, PA; | PSI-7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype inhibitor of hepatitis C virus replication | Antimicrobial agents and chemotherapy | 20516278 | Human bone marrow progenitor cell toxicity assays were performed by Emer Clarke at ReachBio LLC (Seattle, WA) | Full Paper |
2010 | Maloney, JM;Nikova, D;Lautenschläger, F;Clarke, E;Langer, R;Guck, J;Van Vliet, KJ; | Mesenchymal stem cell mechanics from the attached to the suspended state | Biophysical journal | 20959088 | artment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts ¶Department of Physics, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom ‖ReachBio, Seattle, Washington ∗Corresponding author k | Full Paper |
2009 | Nemunaitis, J;Clayman, G;Agarwala, SS;Hrushesky, W;Wells, JR;Moore, C;Hamm, J;Yoo, G;Baselga, J;Murphy, BA;Menander, KA;Licato, LL;Chada, S;Gibbons, RD;Olivier, M;Hainaut, P;Roth, JA;Sobol, RE;Goodwin, WJ; | Biomarkers Predict p53 Gene Therapy Efficacy in Recurrent Squamous Cell Carcinoma of the Head and Neck | Clin. Cancer Res. | 19996201 | iplex primers (Affymetrix, Inc.). The resultant PCR products were analyzed using the Affymetrix p53 GeneChip, which encompasses all coding exons, 2 to 11, as well as immediate flanking regions. Confirmation of the results was carried out by sequencin | Full Paper |
2009 | Mori, F;Oda, N;Sakuragi, M;Sakakibara, F;Kiniwa, M;Miyoshi, K; | New histopathological experimental model for benign prostatic hyperplasia: stromal hyperplasia in rats | J. Urol. | 19095262 | All sections were developed by the avidin-biotin complex method. Sections were also stained with hematoxylin and eosin, and Masson's trichrome. Human BPH tissue samples (Folio Biosciences, Columbus, Ohio) were also stained. Histological Analysis | Full Paper |
2009 | Fazzone, W;Wilson, PM;Labonte, MJ;Lenz, HJ;Ladner, RD; | Histone deacetylase inhibitors suppress thymidylate synthase gene expression and synergize with the fluoropyrimidines in colon cancer cells | Int. J. Cancer | 19384949 | liquid nitrogen or fixed in 10% formalin. Formalin‐fixed tumor specimens were paraffin‐embedded and subsequently analyzed by immunohistochemistry (IHC) (QualTek Laboratories, Newtown, PA). IHC for TS was conducted as | Full Paper |
2009 | Donkor, J;Zhang, P;Wong, S;O'Loughlin, L;Dewald, J;Kok, BP;Brindley, DN;Reue, K; | A conserved serine residue is required for the phosphatidate phosphatase activity but not the transcriptional coactivator functions of lipin-1 and lipin-2 | J. Biol. Chem. | 19717560 | Invitrogen). Plasmids were sequences to select templates for sense and antisense probe generation and linearized by digestion with BamHI. In situ hybridization was performed using the services of Phylogeny, Inc. (Columbus, OH) | Full Paper |
2009 | Maxwell, SA;Li, Z;Jaye, D;Jaya, D;Ballard, S;Ferrell, J;Fu, H; | 14-3-3zeta mediates resistance of diffuse large B cell lymphoma to an anthracycline-based chemotherapeutic regimen | J. Biol. Chem. | 19525224 | 3′. Preparation of DLBCL Tissue Extracts for Western Blotting. Normal and DLBCL lymph node tissues were procured through Folio Biosciences. Frozen tissue was ground to a powder in liquid nitrogen in a pestle and mortar | Full Paper |
2009 | Deroo, BJ;Rodriguez, KF;Couse, JF;Hamilton, KJ;Collins, JB;Grissom, SF;Korach, KS; | Estrogen receptor beta is required for optimal cAMP production in mouse granulosa cells | Mol. Endocrinol. | 19324971 | Mice were treated with vehicle or PMSG for 48 h, after which the ovaries were removed and snap frozen in 2-methylbutane (isopentane) and then stored at −70 C. In situ hybridization analysis for the detection of Lhcgr in whole ovaries was then carried | Full Paper |
2009 | Cunningham, D;Spychala, K;McLarren, KW;Garza, LA;Boerkoel, CF;Herman, GE; | Developmental expression pattern of the cholesterogenic enzyme NSDHL and negative selection of NSDHL-deficient cells in the heterozygous Bpa(1H)/+ mouse | Mol. Genet. Metab. | 19631568 | A darkfield view of a similar section hybridized with a control 35S-labeled sense Nsdhl RNA probe, showing little or no nonspecific background hybridization. (D) A brightfield view of a lateral sagittal section of the E15.5 embryo that includes the e | Full Paper |
2009 | Chen, LM;Verity, NJ;Chai, KX; | Loss of prostasin (PRSS8) in human bladder transitional cell carcinoma cell lines is associated with epithelial-mesenchymal transition (EMT) | BMC Cancer | 19849847 | (Hayward, CA). A Bladder Carcinoma TMA (ARY-HH0087) containing 80 tissue cores, each at 1.5 mm in diameter; with 40 cancer tissues and 40 matching or independent non-cancerous tissues was obtained from Folio Biosciences (Columbus, OH) | Full Paper |
2009 | Binkert, CA;Drooz, AT;Caridi, JG;Sands, MJ;Bjarnason, H;Lynch, FC;Rilling, WS;Zambuto, DA;Stavropoulos, SW;Venbrux, AC;Kaufman, JA; | Technical success and safety of retrieval of the G2 filter in a prospective, multicenter study | J Vasc Interv Radiol | 19875062 | Full Paper | |
2009 | Lynch, FC; | Balloon-assisted removal of tilted inferior vena cava filters with embedded tips | J Vasc Interv Radiol | 19729132 | Full Paper | |
2009 | Lynch, FC;Kekulawela, S; | Removal of the G2 filter: differences between implantation times greater and less than 180 days | J Vasc Interv Radiol | 19640738 | Full Paper | |
2009 | Cantwell, CP;Pennypacker, J;Singh, H;Scorza, LB;Waybill, PN;Lynch, FC; | Comparison of the recovery and G2 filter as retrievable inferior vena cava filters | J Vasc Interv Radiol | 19640733 | Full Paper | |
2009 | Choi, KD;Vodyanik, MA;Slukvin, II; | Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors | The Journal of clinical investigation | 19726877 | CD1a+ DCs, or LCs were cocultured with 1 × 105 adult peripheral (AllCells) or cord blood (Cambrex) mononuclear cells in RPMI 1640 medium (Invitrogen) containing 5% human AB serum (Sigma-Aldrich) in 96-well flat-bottom plates. At 6 days of culture, th | Full Paper |
2009 | Pfeffer, BA;Bernstein, SA;Bartels, SP; | Preservation of structure and immunoreactivity at the vitreoretinal interface of the rabbit eye | Graefes Arch. Clin. Exp. Ophthalmol. | 19020891 | Presented in part at the 2006 meeting of The Association for Research in Vision and Ophthalmology, Fort Lauderdale, Florida. This study was supported by Bausch & Lomb, Inc., with whom QualTek Molecular Laboratories has a contractual relationship | Full Paper |
2009 | Petrie, KR;Schenk, T;Chornet, MB;Howell, L;Dick, JE; | Modulation of Histone H3K4 and H3K27 Methylation Levels Via Pharmacological Inhibition of LSD1 and Degradation of the EZH2-Containing Polycomb Repressive Complex 2 Stimulates ATRA-Mediated Differentiation of AML Cells | Conference | Flow cytometric detection of apoptosis among normal mononuclear cells obtained from the bone marrow of healthy volunteers (AllCells, LLC) by phycoerythrin (PE)conjugated Annexin- Save | Full Paper | |
2009 | Muchamuel, T;Basler, M;Aujay, MA;Suzuki, E;Kalim, KW;Lauer, C;Sylvain, C;Ring, ER;Shields, J;Jiang, J;Shwonek, P;Parlati, F;Demo, SD;Bennett, MK;Kirk, CJ;Groettrup, M; | A selective inhibitor of the immunoproteasome subunit LMP7 blocks cytokine production and attenuates progression of experimental arthritis | Nature medicine | 19525961 | We purchased human PBMCs from normal healthy volunteers from AllCells, and we obtained PBMCs from individuals with rheumatoid arthritis (who had given informed consent) from a Save | Full Paper |
2009 | Majeti, R;Chao, MP;Alizadeh, AA;Pang, WW;Jaiswal, S;Gibbs, KD;van Rooijen, N;Weissman, IL; | CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cells | Cell | 19632179 | Acute myeloid leukemia (AML) is organized as a cellular hierarchy initiated and maintained by a subset of self-renewing leukemia stem cells (LSC). We hypothesized that increased Save | Full Paper |
2009 | Schmitt, N;Morita, R;Bourdery, L;Bentebibel, SE;Zurawski, SM;Banchereau, J;Ueno, H; | Human dendritic cells induce the differentiation of interleukin-21-producing T follicular helper-like cells through interleukin-12 | Immunity | 19592276 | sorting with FACSAria (BD) as CCR7+ CD8- CD56- HLA-DR- CD45RA+ CD4+ cells. Memory CD4+ T cells were directly sorted from PBMCs as CD8- CD56- CD45RA- CD4+ cells. Cord blood naïve CD4 T cells were sorted from frozen cells (AllCells) as CCR7+ CD8- CD56- | Full Paper |
2009 | Wang, Y;Sul, HS; | Pref-1 regulates mesenchymal cell commitment and differentiation through Sox9 | Cell metabolism | 19254573 | Human-derived marrow stromal cells (MSC) and MSC media were purchased from Allcells. Sox9 target-specific siRNA (sc-36534) (Santa Cruz) and the control siRNA (sc-36869) were Save | Full Paper |
2009 | Trifari, S;Kaplan, CD;Tran, EH;Crellin, NK;Spits, H; | Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells | Nature immunology | 19578368 | Naive CD4 + T cells were obtained from human cord blood (AllCells) with a Naive CD4 + T cell Isolation Kit II (Miltenyi), followed by sorting of the CD4 + CD45RA + CD45RO − Save | Full Paper |
2009 | Ye, Z;Zhan, H;Mali, P;Dowey, S;Williams, DM;Jang, YY;Dang, CV;Spivak, JL;Moliterno, AR;Cheng, L; | Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders | Blood | 19797525 | Isolated PB CD34 + cells after G-CSF mobilization were purchased from AllCells and used as a normal control for analyzing gene expression. Four classic retroviral vectors pMXs-Oct4, Save | Full Paper |
2009 | Parlati, F;Lee, SJ;Aujay, M;Suzuki, E;Levitsky, K;Lorens, JB;Micklem, DR;Ruurs, P;Sylvain, C;Lu, Y;Shenk, KD;Bennett, MK; | Carfilzomib can induce tumor cell death through selective inhibition of the chymotrypsin-like activity of the proteasome | Blood | 19671918 | Rosen's laboratory at Northwestern University. CD138+ cells and peripheral blood mononuclear cells (PBMCs) were purchased from Allcells. The purity of CD138+ cells was greater than 90% as determined by the supplier. HS-Sultan-GFPu cell lines were gen | Full Paper |
2009 | Figueroa, ME;Skrabanek, L;Li, Y;Jiemjit, A;Fandy, TE;Paietta, E;Fernandez, H;Tallman, MS;Greally, JM;Carraway, H;Licht, JD;Gore, SD;Melnick, A; | MDS and secondary AML display unique patterns and abundance of aberrant DNA methylation | Blood | 19652201 | Support Laboratory, Cincinnati Children's Hospital, and 4 from Allcells. Matched CD34+ and CD34− fractions were obtained from an additional 4 healthy donors through the Stem Cell and Xenograft Core, University of Pennsylvania School of Medicine | Full Paper |
2009 | Hasstedt, SJ;Bezemer, ID;Callas, PW;Vossen, CY;Trotman, W;Hebbel, RP;Demers, C;Rosendaal, FR;Bovill, EG; | Cell adhesion molecule 1: a novel risk factor for venous thrombosis | Blood | 19643986 | cover slips coated with gelatin (Allcells Inc) under sterile conditions at 37C, 5% CO 2 in HUVEC Basal Medium containing HUVEC Basal Medium Supplement (Allcells Inc). Cells were Save | Full Paper |
2009 | Shi, P;Lai, R;Lin, Q;Iqbal, AS;Young, LC;Kwak, LW;Ford, RJ;Amin, HM; | IGF-IR tyrosine kinase interacts with NPM-ALK oncogene to induce survival of T-cell ALK+ anaplastic large-cell lymphoma cells | Blood | 19423729 | Type I insulin-like growth factor receptor (IGF-IR) tyrosine kinase plays important roles in the pathogenesis of several malignancies. Although it promotes the growth of stimulated Save | Full Paper |
2009 | Loh, YH;Agarwal, S;Park, IH;Urbach, A;Huo, H;Heffner, GC;Kim, K;Miller, JD;Ng, K;Daley, GQ; | Generation of induced pluripotent stem cells from human blood | Blood | 19299331 | obtained from Allcells (Emeryville, CA) were collected from a 26-year-old male donor, and CD34+ cells (mPB014F) were isolated. Generation of CD34 iPS cells was performed using a protocol modified from previously published methods.9 | Full Paper |
2009 | Nahimana, A;Attinger, A;Aubry, D;Greaney, P;Ireson, C;Thougaard, AV;Tjørnelund, J;Dawson, KM;Dupuis, M;Duchosal, MA; | The NAD biosynthesis inhibitor APO866 has potent antitumor activity against hematologic malignancies | Blood | 19196867 | APO866 inhibits nicotinamide phosphoribosyltransferase (NMPRTase), a key enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis from the natural precursor Save | Full Paper |
2009 | Nardi, V;Naveiras, O;Azam, M;Daley, GQ; | ICSBP-mediated immune protection against BCR-ABL-induced leukemia requires the CCL6 and CCL9 chemokines | Blood | 19171873 | eripheral blood mononuclear cells from healthy volunteers were purchased from AllCells (Emeryville, CA) and maintained in complete RPMI medium. Murine and human cells were treated with IFN alpha 2A or IFN beta (R&D Systems | Full Paper |
2009 | Figueroa, ME;Wouters, BJ;Skrabanek, L;Glass, J;Li, Y;Erpelinck-Verschueren, CA;Langerak, AW;Löwenberg, B;Fazzari, M;Greally, JM;Valk, PJ;Melnick, A;Delwel, R; | Genome-wide epigenetic analysis delineates a biologically distinct immature acute leukemia with myeloid/T-lymphoid features | Blood | 19168792 | 4 acquired from the Translational Trials Development and Support Laboratory, Cincinnati Children's Hospital (Cincinnati, OH) and 4 purchased from Allcells (Emeryville, CA). This research was approved by the institutional review boards at Albert Einst | Full Paper |
2009 | Chen, H;Campbell, RA;Chang, Y;Li, M;Wang, CS;Li, J;Sanchez, E;Share, M;Steinberg, J;Berenson, A;Shalitin, D;Zeng, Z;Gui, D;Perez-Pinera, P;Berenson, RJ;Said, J;Bonavida, B;Deuel, TF;Berenson, JR; | Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: a novel mechanism of tumor-induced vasculogenesis | Blood | 19060246 | The human RPMI8226 and U266 MM cell lines and monocyte (THP-1) and myelomonocytic (U937) cell lines were obtained from ATCC (Manassas, VA). Normal human BM was purchased from Allcells (Berkeley, CA). Highly purified human T cells from healthy donors | Full Paper |
2009 | Luo, XM;Maarschalk, E;O'Connell, RM;Wang, P;Yang, L;Baltimore, D; | Engineering human hematopoietic stem/progenitor cells to produce a broadly neutralizing anti-HIV antibody after in vitro maturation to human B lymphocytes | Blood | 19059876 | 90 minutes at 50 000 × g. Human cord blood CD34+ HSPCs were purchased from AllCells (Emeryville, CA) and maintained for less than 24 hours before infection in Iscove modified Dulbecco medium containing 10% fetal bovine serum and cytokines. The cytoki | Full Paper |
2009 | Joshi, I;Minter, LM;Telfer, J;Demarest, RM;Capobianco, AJ;Aster, JC;Sicinski, P;Fauq, A;Golde, TE;Osborne, BA; | Notch signaling mediates G1/S cell-cycle progression in T cells via cyclin D3 and its dependent kinases | Blood | 19001083 | Notch signaling is critical for the regulation of differentiation, proliferation and apoptosis in many cell types. Notch receptors and various Notch ligands have been shown to have a Save | Full Paper |
2009 | Bedi, R;Du, J;Sharma, AK;Gomes, I;Ackerman, SJ; | Human C/EBP-epsilon activator and repressor isoforms differentially reprogram myeloid lineage commitment and differentiation | Blood | 18832658 | Cord blood (CB) CD34+ cells ( 97% pure) purchased from AllCells (Emeryville, CA) were prestimulated for 18 hours at 106 cells/mL in presence of 100 ng/mL recombinant human stem cell factor (rh-SCF), recombinant human fms-like tyrosine kinase 3 ligand | Full Paper |
2009 | Oda, M;Glass, JL;Thompson, RF;Mo, Y;Olivier, EN;Figueroa, ME;Selzer, RR;Richmond, TA;Zhang, X;Dannenberg, L;Green, RD;Melnick, A;Hatchwell, E;Bouhassira, EE;Verma, A;Suzuki, M;Greally, JM; | High-resolution genome-wide cytosine methylation profiling with simultaneous copy number analysis and optimization for limited cell numbers | Nucleic acids research | 19386619 | One million fresh CD34 + bone marrow progenitors were purchased from Allcells (Emerville, CA). DNA was extracted from these cells and checked for purity and amount by Save | Full Paper |
2009 | O'Brien, JJ;Baglole, CJ;Garcia-Bates, TM;Blumberg, N;Francis, CW;Phipps, RP; | 15-deoxy-Delta12,14 prostaglandin J2-induced heme oxygenase-1 in megakaryocytes regulates thrombopoiesis | Journal of thrombosis and haemostasis : JTH | 18983509 | Human CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5×105 cells per well in a 12-well plate and cultured in serum-free medium as previously described (19) and supplemented with 100 ng/mL of recombinant hu | Full Paper |
2009 | Liu, PC;Caulder, E;Li, J;Waeltz, P;Margulis, A;Wynn, R;Becker-Pasha, M;Li, Y;Crowgey, E;Hollis, G;Haley, P;Sparks, RB;Combs, AP;Rodgers, JD;Burn, TC;Vaddi, K;Fridman, JS; | Combined inhibition of Janus kinase 1/2 for the treatment of JAK2V617F-driven neoplasms: selective effects on mutant cells and improvements in measures of disease severity | Clinical cancer research : an official journal of the American Association for Cancer Research | 19887489 | Mononuclear cells were isolated from peripheral blood samples from patients with PV or normal control individuals by centrifugation through Ficoll (Allcells, Inc.). Colony forming assays Save | Full Paper |
2009 | Cardarelli, PM;Moldovan-Loomis, MC;Preston, B;Black, A;Passmore, D;Chen, TH;Chen, S;Liu, J;Kuhne, MR;Srinivasan, M;Assad, A;Witte, A;Graziano, RF;King, DJ; | In vitro and in vivo characterization of MDX-1401 for therapy of malignant lymphoma | Clinical cancer research : an official journal of the American Association for Cancer Research | 19401346 | ADCC assay. Human peripheral blood mononuclear effector cells were purified from either heparinized whole blood (AllCells) or buffy coat (Stanford Blood Center) by standard Ficoll-Paque separation. Cells (2 × 106) were washed in PBS and sent for geno | Full Paper |
2009 | Zhong, C;Qu, X;Tan, M;Meng, YG;Ferrara, N; | Characterization and regulation of bv8 in human blood cells | Clinical cancer research : an official journal of the American Association for Cancer Research | 19336519 | Isolation of whole human bone marrow cells from fresh bone marrow collections. Fresh bone marrow samples were obtained from ALLCELLS. The bone marrow was first diluted with 10% fetal bovine serum-containing DMEM and then passed through a 40-μm cell s | Full Paper |
2009 | Ara, T;Song, L;Shimada, H;Keshelava, N;Russell, HV;Metelitsa, LS;Groshen, SG;Seeger, RC;DeClerck, YA; | Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells | Cancer research | 19118018 | Human BMSC were either purchased from ALLCells LLC or obtained from bone marrow samples of patients with neuroblastoma enrolled by the Children's Oncology Group. These Save | Full Paper |
2009 | Senyuk, V;Rinaldi, CR;Li, D;Cattaneo, F;Stojanovic, A;Pane, F;Du, X;Mahmud, N;Dickstein, J;Nucifora, G; | Consistent up-regulation of Stat3 Independently of Jak2 mutations in a new murine model of essential thrombocythemia | Cancer research | 19118011 | Mononuclear cells were separated from the BM aspirates by Ficoll-Hypaque centrifugation. The samples were obtained according to guidelines established by the University of Illinois at Chicago Institutional Review Board. Healthy donors’ BM cells were | Full Paper |
2009 | Kim, YC;Wu, Q;Chen, J;Xuan, Z;Jung, YC;Zhang, MQ;Rowley, JD;Wang, SM; | The transcriptome of human CD34+ hematopoietic stem-progenitor cells | Proceedings of the National Academy of Sciences of the United States of America | 19416867 | De Novo Collection of CD34+ 3′ ESTs. Bone marrow CD34+ cells of 3 healthy donors were purchased from AllCells. 3′ ESTs were collected by using the GLGI method (18, 19). A total of 10,000 CD34+ SAGE tags identified in a previous study (12) were select | Full Paper |
2009 | Majeti, R;Becker, MW;Tian, Q;Lee, TL;Yan, X;Liu, R;Chiang, JH;Hood, L;Clarke, MF;Weissman, IL; | Dysregulated gene expression networks in human acute myelogenous leukemia stem cells | Proceedings of the National Academy of Sciences of the United States of America | 19218430 | Normal human bone marrow mononuclear cells were either purchased from AllCells or obtained from National Marrow Donor Program discarded filter units. For AML specimens, peripheral blood and/or bone marrow was obtained following informed consent at th | Full Paper |
2009 | Bernard, JJ;Seweryniak, KE;Koniski, AD;Spinelli, SL;Blumberg, N;Francis, CW;Taubman, MB;Palis, J;Phipps, RP; | Foxp3 regulates megakaryopoiesis and platelet function | Arteriosclerosis, thrombosis, and vascular biology | 19661482 | Human CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5×105 cells per well in a 12-well plate and cultured in serum-free medium as previously described 12 and supplemented with 100 ng/mL of recombinant huma | Full Paper |
2009 | Jekle, A;Chow, E;Kopetzki, E;Ji, C;Yan, MJ;Nguyen, R;Sankuratri, S;Cammack, N;Heilek, G; | CD4-BFFI: a novel, bifunctional HIV-1 entry inhibitor with high and broad antiviral potency | Antiviral research | 19559732 | obtained from ATCC, Manassas, VA. Human PBMC were obtained from AllCells (Emeryville, CA), stimulated for one day in PBMC media (RPMI-1640 media containing 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 2 mM l-glutamine, 1 mM sodium-pyruva | Full Paper |
2009 | Nakahara, K;Wakasa-Morimoto, C;Kobayashi, M;Miki, S;Noshi, T;Seki, T;Kanamori-Koyama, M;Kawauchi, S;Suyama, A;Fujishita, T;Yoshinaga, T;Garvey, EP;Johns, BA;Foster, SA;Underwood, MR;Sato, A;Fujiwara, T; | Secondary mutations in viruses resistant to HIV-1 integrase inhibitors that restore viral infectivity and replication kinetics | Antiviral research | 19027039 | Passage of HIV-1 in the presence of integrase inhibitors (INIs) generates resistant viruses that have mutations in the integrase region. Integrase-resistant mutations Q148K and Q148R Save | Full Paper |
2009 | Strickland, LA;Ross, J;Williams, S;Ross, S;Romero, M;Spencer, S;Erickson, R;Sutcliffe, J;Verbeke, C;Polakis, P;van Bruggen, N;Koeppen, H; | Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinoma | The Journal of pathology | 19334050 | consenting donors; bone marrow samples and purified CD34+ cells (AllCells Inc., Berkeley, CA, USA) were received within 5 (unfractionated samples) or 24 (fractionated samples) h of collection. PC3 (CRL-1435) and HPAFII (CRL-2119) cells were obtained | Full Paper |
2009 | Leleu, X;Hunter, ZR;Xu, L;Roccaro, AM;Moreau, AS;Santos, DD;Hatjiharissi, E;Bakthavachalam, V;Adamia, S;Ho, AW;Soumerai, J;Patterson, CJ;Manning, RJ;Hamilton, S;Verselis, S;Fox, E;Carrasco, R;Ghobrial, IM;Treon, SP; | Expression of regulatory genes for lymphoplasmacytic cell differentiation in Waldenstrom Macroglobulinemia | British journal of haematology | 19220283 | years and the male/female ratio was 1·6. All WM patients were previously untreated. Table I summarizes the characteristics of the patients. Bone marrow aspirates from healthy donors were used in comparison (AllCells LLC, Berkeley, CA, USA) | Full Paper |
2009 | Krupa, A;Fudala, R;Stankowska, D;Loyd, T;Allen, TC;Matthay, MA;Gryczynski, Z;Gryczynski, I;Mettikolla, YV;Kurdowska, AK; | Anti-chemokine autoantibody:chemokine immune complexes activate endothelial cells via IgG receptors | American journal of respiratory cell and molecular biology | 19109244 | HUVECs were purchased from Cambrex (Walkersville, MD) or Allcells (Berkeley, CA), and cultured in endothelial basal medium containing endothelial growth factor, hydrocortisone, gentamicin, amphoreticin B, bovine brain extract, heparin, and FBS (EGM-M | Full Paper |
2009 | Lee, JH;Ort, T;Ma, K;Picha, K;Carton, J;Marsters, PA;Lohmander, LS;Baribaud, F;Song, XY;Blake, S; | Resistin is elevated following traumatic joint injury and causes matrix degradation and release of inflammatory cytokines from articular cartilage in vitro | Osteoarthritis and cartilage | 19095472 | Human primary monocytes isolated from three normal healthy volunteers were purchased from AllCells, LLC (Emeryville, CA). The cells were treated with recombinant human resistin (Peprotech, Inc, Rocky Hill, NJ; endotoxin level was measured as 0.9 EU/m | Full Paper |
2009 | Hashimoto, S;Nishiyama, T;Hayashi, S;Takebe, K;Kanzaki, N;Fujishiro, T;Kuroda, R;Kurosaka, M; | 224 THE FUNCTION OF p53 ON HUMAN CHONDROCYTE APOPTOSIS IN RESPONSE TO SHEAR STRESS | Osteoarthritis and Cartilage | ated by enzymatic digestion of bone from patients undergoing knee endoprothesis due to arthrosis of the knee joint. Human macrophages were differentiated from CD14+ peripheral blood monocytes (Allcells) by treatment with macrophage colony | Full Paper | |
2009 | Peterson, SJ;Kim, DH;Li, M;Positano, V;Vanella, L;Rodella, LF;Piccolomini, F;Puri, N;Gastaldelli, A;Kusmic, C;L'Abbate, A;Abraham, NG; | The L-4F mimetic peptide prevents insulin resistance through increased levels of HO-1, pAMPK, and pAKT in obese mice | Journal of lipid research | 19224872 | at various time points (0-120 min) for measurement of blood glucose levels. Human bone marrow-derived mesenchymal stem cells and adipocytes Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing the cells, | Full Paper |
2009 | Patel, E;Wang, B;Lien, L;Wang, Y;Yang, LJ;Moreb, JS;Chang, LJ; | Diverse T-cell differentiation potentials of human fetal thymus, fetal liver, cord blood and adult bone marrow CD34 cells on lentiviral Delta-like-1-modified mouse stromal cells | Immunology | 19740310 | Invitrogen, Carlsbad, CA) supplemented with 20% fetal bovine serum and 1% penicillin-streptomycin. The adult BM and CB CD34+ cells were purchased from AllCells, LLC (Emeryville, CA), and Cambrex Corp. (Baltimore, MD) Aborted fetal liver and thymus ti | Full Paper |
2009 | Sutherland, MK;Yu, C;Lewis, TS;Miyamoto, JB;Morris-Tilden, CA;Jonas, M;Sutherland, J;Nesterova, A;Gerber, HP;Sievers, EL;Grewal, IS;Law, CL; | Anti-leukemic activity of lintuzumab (SGN-33) in preclinical models of acute myeloid leukemia | mAbs | 20065652 | Primary normal human monocytes and primary AML patient bone marrow samples were obtained from AllCells (Emeryville, CA). Fluorochrome-conjugated antibodies against human Save | Full Paper |
2009 | Nan, JL;Li, JJ;He, JG; | C-reactive protein decreases interleukin-8 production in human endothelial progenitor cells by inhibition of p38 MAPK pathway | Chinese medical journal | 19781372 | Theabilityto modulate the antigenic pbenotype in response to the environmentiscommon to allcells.ECsmay regulate 1evelsofvascularendothelialgrowthfactorreceptor一2 in response to Save | Full Paper |
2009 | Patterson, ST;Li, J;Kang, JA;Wickrema, A;Williams, DB;Reithmeier, RA; | Loss of specific chaperones involved in membrane glycoprotein biosynthesis during the maturation of human erythroid progenitor cells | The Journal of biological chemistry | 19258325 | hematopoietic progenitors were either purchased from Stem Cell Technologies (mPB015F) or initially isolated from growth factor-mobilized peripheral blood (purchased from AllCells, Save | Full Paper |
2009 | Ji, C;Kopetzki, E;Jekle, A;Stubenrauch, KG;Liu, X;Zhang, J;Rao, E;Schlothauer, T;Fischer, S;Cammack, N;Heilek, G;Ries, S;Sankuratri, S; | CD4-anchoring HIV-1 fusion inhibitor with enhanced potency and in vivo stability | The Journal of biological chemistry | 19097993 | In this study, we describe a novel CD4-targeting bifunctional human immunodeficiency virus (HIV-1) fusion inhibitor (CD4-BFFI) that blocks HIV-1 entry by inhibiting both HIV-1 Save | Full Paper |
2009 | Hirschler-Laszkiewicz, I;Tong, Q;Conrad, K;Zhang, W;Flint, WW;Barber, AJ;Barber, DL;Cheung, JY;Miller, BA; | TRPC3 activation by erythropoietin is modulated by TRPC6 | The Journal of biological chemistry | 19074769 | Human erythroid progenitors were also cultured using a two-phase liquid culture system (44, 45). Cells were harvested at day 8 of phase II and were predominantly proerythroblasts or basophilic normoblasts. CD34+ cells were purchased from AllCells, LL | Full Paper |
2009 | Zhang, Q;Shi, S;Liu, Y;Uyanne, J;Shi, Y;Shi, S;Le, AD; | Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis | Journal of immunology (Baltimore, Md. : 1950) | 19923445 | Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells and cultured with α-MEM supplemented with 10% FBS, 100 μM l-ascorbic acid-2-phosphate, 2 mM l-glutamine, 100 U/ml penicillin, and 100 μg/ml strept | Full Paper |
2009 | Parrish, YK;Baez, I;Milford, TA;Benitez, A;Galloway, N;Rogerio, JW;Sahakian, E;Kagoda, M;Huang, G;Hao, QL;Sevilla, Y;Barsky, LW;Zielinska, E;Price, MA;Wall, NR;Dovat, S;Payne, KJ; | IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrow | Journal of immunology (Baltimore, Md. : 1950) | 19299724 | CB was collected according to guidelines approved by the Institutional Review Boards at Childrens Hospital Los Angeles and Loma Linda University. Adult human BM was purchased from AllCells, LLC, Berkeley, CA; the National Disease Research Interchange | Full Paper |
2009 | Vendel, AC;Calemine-Fenaux, J;Izrael-Tomasevic, A;Chauhan, V;Arnott, D;Eaton, DL; | B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK | Journal of immunology (Baltimore, Md. : 1950) | 19155498 | Tonsil tissue (AllCells) was dissociated and a single-cell suspension was stained with anti-CD19, anti-IgD, and anti-CD38. Naive tonsillar B cells were identified as CD19 + IgD + CD38 Save | Full Paper |
2009 | Panetta, NJ;Gupta, DM;Kwan, MD;Wan, DC;Commons, GW;Longaker, MT; | Tissue harvest by means of suction-assisted or third-generation ultrasound-assisted lipoaspiration has no effect on osteogenic potential of human adipose-derived stromal cells | Plastic and reconstructive surgery | 19568046 | Commercially available bone marrow mesenchymal stem cells (AllCells), subjected to similar experimental osteogenic differentiation conditions, demonstrated significantly less staining Save | Full Paper |
2009 | Weigel-Van Aken, KA; | Pharmacological activation of guanine nucleotide exchange factors for the small GTPase Rap1 recruits high-affinity beta1 integrins as coreceptors for parvovirus B19: improved ex vivo gene transfer to human erythroid progenitor cells | Human gene therapy | 19702438 | Human bone marrow-derived CD36 + erythroid progenitor cells were purchased from AllCells (Emeryville, CA). Cells were cultured overnight in serum-free medium containing stem cell Save | Full Paper |
2009 | Zinckgraf, JW;Sposato, M;Zielinski, V;Powell, D;Treanor, JJ;von Hofe, E; | Identification of HLA class II H5N1 hemagglutinin epitopes following subvirion influenza A (H5N1) vaccination | Vaccine | 19596415 | Since we did not have access to pre-vaccine samples, eight random naive donor PBMC samples (AllCells, Emeryville, CA) were utilized to assess potential cross-reactive T cell Save | Full Paper |
2009 | Morizono, K;Pariente, N;Xie, Y;Chen, IS; | Redirecting lentiviral vectors by insertion of integrin-tageting peptides into envelope proteins | The journal of gene medicine | 19434609 | Human umbilical vein endothelial cells (HUVEC) were purchased from AllCells (Emeryville, CA, USA) and Lonza (Walkersville, MD, USA). HUVEC were cultured in EBM-2 (Lonza) supplemented with EGM-2 singlequots (Lonza). When HUVEC started growing slowly, | Full Paper |
2009 | Liang, M;Pariente, N;Morizono, K;Chen, IS; | Targeted transduction of CD34+ hematopoietic progenitor cells in nonpurified human mobilized peripheral blood mononuclear cells | The journal of gene medicine | 19152374 | Cells binding with the anti-CD34 antibody were separated by a MACS machine (Miltenyi Biotec). Human primary PBMCs were isolated from leukopacks, Ficoll purified and cultured in RPMI with 20% FCS (Omega Scientific, Tarzana, CA, USA). Frozen human mobi | Full Paper |
2009 | Lee, CH;Marion, NW;Hollister, S;Mao, JJ; | Tissue formation and vascularization in anatomically shaped human joint condyle ectopically in vivo | Tissue engineering. Part A | 19563263 | hMSCs were isolated from fresh bone marrow samples of multiple anonymous adult donors (AllCells, Berkeley, CA) per our prior methods. Purification of hMSCs was accomplished by Save | Full Paper |
2009 | 李文芳, ;黄元庆, ; | 基于虚拟仪器和 DDE 发电机组温度实时数据采集系统的研制 | 厦门大学学报 (自然科学版) | 其对应的 DDE 服务名,主题名和项名应用与服务器端一致, 分 别是"wd" ,"DDE wd Test"和"AllCells" .在LabWindows/CVI 实时数据库中, 操作的对象是点AllCells , 是一组油温参数的集合, 可以 Save | Full Paper | |
2009 | Hall, AA;Leonardo, CC;Collier, LA;Rowe, DD;Willing, AE;Pennypacker, KR; | Delayed treatments for stroke influence neuronal death in rat organotypic slice cultures subjected to oxygen glucose deprivation | Neuroscience | 19723565 | HUCBC (AllCells, LLC, Inc.) were thawed and resuspended in DMEM containing DNase (1ug/ml) to prevent aggregation. HUCBC were washed twice and resuspended at a concentration of 108 cells/ml. Cultures were maintained in a standard tissue culture incuba | Full Paper |
2009 | Fukushima-Shintani, M;Suzuki, K;Iwatsuki, Y;Abe, M;Sugasawa, K;Hirayama, F;Kawasaki, T;Nakahata, T; | AKR-501 (YM477) a novel orally-active thrombopoietin receptor agonist | European journal of haematology | 19183407 | Human cord blood (CB) CD34+ cells (AllCells, Berkeley, CA, USA) at a density of 2500 cells/0.75 mL were suspended in MegaCult™-C (StemCell Technologies Inc., Vancouver, Canada) containing either AKR-501 dissolved in DMSO or rhTPO, and plated in two-w | Full Paper |
2009 | Zhao, Z;He, X;Bi, Y;Xia, Y;Tao, N;Li, L;Ma, Q; | Induction of CYP4F3 by benzene metabolites in human white blood cells in vivo in human promyelocytic leukemic cell lines and ex vivo in human blood neutrophils | Drug metabolism and disposition: the biological fate of chemicals | 19029204 | Whole blood from a healthy male adult was purchased from AllCells, LLC (Emeryville, CA). Neutrophils were isolated according a protocol provided by AllCells, LLC. In brief, whole Save | Full Paper |
2009 | Nishino, T;Miyaji, K;Ishiwata, N;Arai, K;Yui, M;Asai, Y;Nakauchi, H;Iwama, A; | Ex vivo expansion of human hematopoietic stem cells by a small-molecule agonist of c-MPL | Experimental hematology | 19744539 | 0.5 U/mL rhEPO, and 10 ng/mL rhTPO, respectively. Human cord blood (hCB) CD34+ cells were purchased from Lonza (Basel, Switzerland) and AllCells (Berkeley, CA, USA) or purified from hCB. Fresh hCB cells were obtained from the Tokyo Cord Blood Bank (T | Full Paper |
2009 | Giammona, LM;Panuganti, S;Kemper, JM;Apostolidis, PA;Lindsey, S;Papoutsakis, ET;Miller, WM; | Mechanistic studies on the effects of nicotinamide on megakaryocytic polyploidization and the roles of NAD+ levels and SIRT inhibition | Experimental hematology | 19715739 | Cultures were initiated in T-flasks with previously frozen mPB CD34-selected cells (AllCells; Berkeley, CA or Fred Hutchinson Cancer Research Center; Seattle, WA) and maintained at a concentration of 100,000 to 300,000 cells/ml as described [8]. Most | Full Paper |
2009 | Gibson, JD;Jakuba, CM;Boucher, N;Holbrook, KA;Carter, MG;Nelson, CE; | Single-cell transcript analysis of human embryonic stem cells | Integrative biology : quantitative biosciences from nano to macro | 20023769 | We demonstrate the qualitative and quantitative power of single-cell transcript analysis to characterize transcriptome dynamics in human embryonic stem cells (hESC's). Single-cell Save | Full Paper |
2009 | Wells, R;Guth, A;Lappin, M;Dow, S; | Anti-endothelial cell antibodies in dogs with immune-mediated hemolytic anemia and other diseases associated with high risk of thromboembolism | Journal of veterinary internal medicine | 19192144 | AllCells LLC, Emeryville, CA bSigma Aldrich, St Louis, MO cGibco-InVitrogen, San Diego, CA dHyclone, Logan, UT eJackson ImmunoResearch, West Grove, PA fDako-Cytomation, Fort Collins, CO | Full Paper |
2009 | Harriman, WD;Collarini, EJ;Cromer, RG;Dutta, A;Strandh, M;Zhang, F;Kauvar, LM; | Multiplexed Elispot assay | Journal of immunological methods | 19084532 | Normal human PBMCs were obtained from AllCells (Emeryville, CA). For CellSpot assays, 105 PBMC, Th1 or Th2 selected cells were suspended in activation medium with phorbol myristate acetate (PMA) at 50 ng/mL and Ionomycin at 1 µg/mL or control medium, | Full Paper |
2009 | Sugiyama, E;Lee, SJ;Lee, SS;Kim, WY;Kim, SR;Tohkin, M;Hasegawa, R;Okuda, H;Kawamoto, M;Kamatani, N;Sawada, J;Kaniwa, N;Saito, Y;Shin, JG; | Ethnic differences of two non-synonymous single nucleotide polymorphisms in CDA gene | Drug metabolism and pharmacokinetics | 20045991 | DNA from 200 healthy Chinese-Americans was extracted from cord blood samples purchased from AllCells (Emeryville, CA, USA). Peripheral blood samples from healthy Caucasian- Save | Full Paper |
2009 | Ye, D;Garcia-Manero, G;Kantarjian, HM;Xiao, L;Vadhan-Raj, S;Fernandez, MH;Nguyen, MH;Medeiros, LJ;Bueso-Ramos, CE; | Analysis of Aurora kinase A expression in CD34(+) blast cells isolated from patients with myelodysplastic syndromes and acute myeloid leukemia | Journal of hematopathology | 19669217 | reverse transcription efficiency. Relative RNA level was reported via standard delta delta Ct (δδ Ct). cDNA purified from normal CD34+ hematopoietic progenitor cells was used as calibrator sample (Allcells, LLC, Emeryville, CA, USA). Immunocytochemi | Full Paper |
2009 | Nagel, I; | Identifizierung von pathogenetisch relevanten Genen bei lymphatischen Neoplasien unter besonderer Berücksichtigung des langen Arms von Chromosom 14 | Thesis | Probanden isoliert. Zur Normalisierung der quantitativen Reverse Transkription (RT)-PCR-Analyse des TRAF3-Gens wurde „Normal Peripheral Blood B Cells cDNA (CD19+)“ der Firma Allcells (Emeryville, CA, USA) verwendet. Für die quantitativen RT-PCR-Analy | Full Paper | |
2009 | Mahadevan, D;Choi, J;Cooke, L;Simons, B;Riley, C;Klinkhammer, T;Sud, R;Maddipoti, S;Hehn, S;Garewal, H;Spier, C; | Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3 and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival and Migration | Human genomics and proteomics : HGP | 20981323 | CD19+ normal PB B-cell RNA from 4 normal volunteers was purchased from AllCells, CA. Control RNA was obtained from normal peripheral (PB) B-cells from 4 volunteers (AllCells, CA) Save | Full Paper |
2009 | Garzon, R; | MicroRNA profiling of megakaryocytes | Methods in molecular biology (Clifton, N.J.) | 18839118 | It is critical to use high-quality CD34+ stem cells. These cells could be obtained commercially from Allcells.com or Stemcell technologies. Other cheaper alternatives include the use of cord blood-selected CD34+ cells. Failure to use high-quality pro | Full Paper |
2009 | Huynh, H;Kaba, M;Rudra, S;Zheng, J;Wu, C;Lodish, H;Zhang, C; | IGFBP2 Supports ex vivo Expansion of Hematopoietic Stem Cells | Research and Perspectives in Endocrine Interactions | Fresh and cryopreserved human cord blood cells were purchased from Cambrex, StemCell Technologies Inc., and AllCells. All of the cells were from pooled donors. CD34 + or CD133 Save | Full Paper | |
2009 | Harirchian, P; | Validation of EBI2 as a Novel Therapeutic Target for Autoimmune Disorders | Thesis | Fresh leukapheresed blood (300 mL) from healthy donors was purchased from Allcells (Emeryville, CA). On the same day of leukapheresis, peripheral blood mononuclear cells (PBMC) Save | Full Paper | |
2009 | Bachstetter, AD; | The role of neuroinflammation in regulating the age-related decline in neurogenesis | Thesis | Cell preparation: Cryopreserved human umbilical cord blood mononuclear cells (UCBMCs) were obtained from Saneron CCEL Therapeutics, Inc. (Tampa, FL, USA). Cryopreserved Human peripheral blood cells (PBMC) (mononuclear fraction) were obtained from All | Full Paper | |
2009 | Carter, CC; | Infection of hematopoietic progenitor cells by HIV-1 | Thesis | Cell culture. Fresh whole bone marrow aspirates were obtained commercially (AllCells Ltd.). Umbilical cord blood was obtained following scheduled cesarean section procedures, in accordance with an IRB-approved protocol. Bone marrow mononuclear cells | Full Paper | |
2009 | O'Brien, JJ; | Mechanisms of megakaryopoiesis and platelet production: Implications for inflammation and disease | Thesis | Human CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5x10" cells per well in a 12-well plate and cultured in serum-free medium as previously described (Zeuner et al. 2007) and supplemented with 100 ng/mL o | Full Paper | |
2009 | Hall, AA; | Immunomodulatory effects of novel therapies for stroke | Thesis | DMEM without glucose (Invitrogen, Carlsbad, CA) for OGD. HUCBC (AllCells) were thawed and resuspended in DMEM containing DNase (1ug/ml) to prevent aggregation. HUCBC were washed twice and resuspended at a concentration of 108 cells/ml. HUCBC (106 ) | Full Paper | |
2009 | Schwengberg, S; | Use of embryonic stem cell derived cardiomyocytes for determination of cardiac cytotoxicity | Journal of Pharmacological and Toxicological Methods | Hescheler for providing Patch Clamp analysis; Leonard Fink for development and programming of the automated analysis software; our colleagues at Axiogenesis AG and ReachBio Save | Full Paper | |
2008 | Ramalingam, S;Forster, J;Naret, C;Evans, T;Sulecki, M;Lu, H;Teegarden, P;Weber, MR;Belani, CP; | Dual inhibition of the epidermal growth factor receptor with cetuximab, an IgG1 monoclonal antibody, and gefitinib, a tyrosine kinase inhibitor, in patients with refractory non-small cell lung cancer (NSCLC): a phase I study | J Thorac Oncol | 18317068 | 38. FFPE tumor samples were submitted to QualTek (Newtown, PA) for EGFR expression analysis by immunohistochemistry. The intensity of staining was scored as: 3 = high, 2 = moderate, 1 = low/minimal, and 0 = no staining. RESULTS. Patient Demographics | Full Paper |
2008 | Jiang, P;Enomoto, A;Jijiwa, M;Kato, T;Hasegawa, T;Ishida, M;Sato, T;Asai, N;Murakumo, Y;Takahashi, M; | An actin-binding protein Girdin regulates the motility of breast cancer cells | Cancer Res. | 18316593 | Tissue arrays of human malignant tumors and matched normal adjacent tissues were purchased from Folio Biosciences, Inc. Immunohistochemisty was performed using standard techniques. Antigen retrieval was performed with microwave treatment in a 0.01 mo | Full Paper |
2008 | Lu, Y;Wang, J;Xu, Y;Koch, AE;Cai, Z;Chen, X;Galson, DL;Taichman, RS;Zhang, J; | CXCL16 functions as a novel chemotactic factor for prostate cancer cells in vitro | Mol. Cancer Res. | 18344492 | High-density tissue microarrays were constructed by Folio Biosciences with clinical samples obtained from a cohort of 80 patients. Tumors were graded using the Gleason grading system and examined to identify areas of benign prostate, prostate cancer, | Full Paper |
2008 | Wang, J;Shiozawa, Y;Wang, J;Wang, Y;Jung, Y;Pienta, KJ;Mehra, R;Loberg, R;Taichman, RS; | The role of CXCR7/RDC1 as a chemokine receptor for CXCL12/SDF-1 in prostate cancer | J. Biol. Chem. | 18057003 | tumor size, or clinical outcome. Breast cancer arrays were purchased from Folio Biosciences (Columbus, OH) (ARY-HH0058), which were generated from clinical samples derived from a cohort of 80 patients. In this case an | Full Paper |
2008 | Gallagher, J;Lynch, FW;Barragry, J; | A prolactinoma masked by a herbal remedy | Eur. J. Obstet. Gynecol. Reprod. Biol. | 17298863 | Full Paper | |
2008 | Meszaros, R;Strate, I;Pera, EM;Durbeej, M; | Expression of the novel gene Ened during mouse and Xenopus embryonic development | Int. J. Dev. Biol. | 18956345 | Mul- tiple alignment of the amino acid sequences was carried out with MultAlin software (http://bioinfo.genopole-toulouse.prd.fr/multalin/multalin.html). Whole-mount and radioactive in situ hybridization (Phylogeny, Inc. Columbus | Full Paper |
2008 | Kawada, M;Inoue, H;Arakawa, M;Ikeda, D; | Transforming growth factor-β1 modulates tumor-stromal cell interactions of prostate cancer through insulin-like growth factor-I | Anticancer research | Immunohistochemistry. Tissue arrays of human prostate cancer were purchased from Folio Biosciences (Columbus, OH, USA). The tissue sections were deparaffinized in xylene and rehydrated in descending alcohols into water | Full Paper | |
2008 | Cherry, RA;Nichols, PA;Snavely, TM;David, MT;Lynch, FC; | Prophylactic inferior vena cava filters: do they make a difference in trauma patients? | J Trauma | 18784566 | Full Paper | |
2008 | Yang, L;Anderson, DE;Baecher-Allan, C;Hastings, WD;Bettelli, E;Oukka, M;Kuchroo, VK;Hafler, DA; | IL-21 and TGF-beta are required for differentiation of human T(H)17 cells | Nature | 18469800 | PBMCs were obtained from the peripheral blood of healthy subjects or from cord blood (AllCells) in compliance with institutional review board (IRB) protocols. CD4 + T cells were Save | Full Paper |
2008 | Cisse, B;Caton, ML;Lehner, M;Maeda, T;Scheu, S;Locksley, R;Holmberg, D;Zweier, C;den Hollander, NS;Kant, SG;Holter, W;Rauch, A;Zhuang, Y;Reizis, B; | Transcription factor E2-2 is an essential and specific regulator of plasmacytoid dendritic cell development | Cell | 18854153 | Total RNA from purified human peripheral blood cell types (Allcells) or sorted murine cells was reverse transcribed and assayed by SYBR Green-based real-time PCR with MX3000P Save | Full Paper |
2008 | Paoluzzi, L;Gonen, M;Bhagat, G;Furman, RR;Gardner, JR;Scotto, L;Gueorguiev, VD;Heaney, ML;Manova, K;O'Connor, OA; | The BH3-only mimetic ABT-737 synergizes the antineoplastic activity of proteasome inhibitors in lymphoid malignancies | Blood | 18591385 | Mononuclear cells from peripheral blood samples (PBMCs) of healthy donors were purchased from Allcells (Emeryville, CA). All cell lines were grown as previously described. Save | Full Paper |
2008 | Luqman, M;Klabunde, S;Lin, K;Georgakis, GV;Cherukuri, A;Holash, J;Goldbeck, C;Xu, X;Kadel, EE;Lee, SH;Aukerman, SL;Jallal, B;Aziz, N;Weng, WK;Wierda, W;O'Brien, S;Younes, A; | The antileukemia activity of a human anti-CD40 antagonist antibody, HCD122, on human chronic lymphocytic leukemia cells | Blood | 18497318 | B-cell chronic lymphocytic leukemia (B-CLL) is a lymphoproliferative disorder characterized by the surface expression of CD20, CD5 antigens, as well as the receptor CD40. Activation Save | Full Paper |
2008 | Zhang, CC;Kaba, M;Iizuka, S;Huynh, H;Lodish, HF; | Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantation | Blood | 18202223 | Fresh and cryopreserved human cord blood cells were purchased from Cambrex (East Rutherford, NJ), StemCell Technologies, and AllCells (Berkeley, CA). All of cells were from pooled donors. CD34+ or CD133+ purity checked by flow cytometry was higher th | Full Paper |
2008 | Garzon, R;Volinia, S;Liu, CG;Fernandez-Cymering, C;Palumbo, T;Pichiorri, F;Fabbri, M;Coombes, K;Alder, H;Nakamura, T;Flomenberg, N;Marcucci, G;Calin, GA;Kornblau, SM;Kantarjian, H;Bloomfield, CD;Andreeff, M;Croce, CM; | MicroRNA signatures associated with cytogenetics and prognosis in acute myeloid leukemia | Blood | 18187662 | from 4 healthy donors were purchased from Allcells (Emeryville, CA). Bone marrow CD34 + progenitors from 10 healthy donors were purchased from Allcells. In vitro differentiated Save | Full Paper |
2008 | Mercher, T;Cornejo, MG;Sears, C;Kindler, T;Moore, SA;Maillard, I;Pear, WS;Aster, JC;Gilliland, DG; | Notch signaling specifies megakaryocyte development from hematopoietic stem cells | Cell stem cell | 18786418 | using a FACSAria (BD Biosciences). Human CD34+ bone marrow cells were obtained from AllCells. For in vitro cocultures of LSK cells with stroma, analyses were gated on forward/side scatter profile and CD45+ or CD45+GFP+ cells. Apoptosis assays were pe | Full Paper |
2008 | Tuller, T;Kupiec, M;Ruppin, E; | Evolutionary rate and gene expression across different brain regions | Genome biology | 18811952 | names and other properties appears in Additional data file 12. The human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA, USA), Clontech (Palo Alto, CA, USA), AllCells (Berkeley, CA, USA), Clonetics/BioWhittak | Full Paper |
2008 | Song, W;van der Vliet, HJ;Tai, YT;Prabhala, R;Wang, R;Podar, K;Catley, L;Shammas, MA;Anderson, KC;Balk, SP;Exley, MA;Munshi, NC; | Generation of antitumor invariant natural killer T cell lines in multiple myeloma and promotion of their functions via lenalidomide: a strategy for immunotherapy | Clinical cancer research : an official journal of the American Association for Cancer Research | 18980990 | Normal bone marrow samples were purchased from AllCells. Multiple myeloma patient blood and bone marrow samples were obtained from Dana-Farber Cancer Institute following Save | Full Paper |
2008 | Lu, J;Guo, S;Ebert, BL;Zhang, H;Peng, X;Bosco, J;Pretz, J;Schlanger, R;Wang, JY;Mak, RH;Dombkowski, DM;Preffer, FI;Scadden, DT;Golub, TR; | MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte progenitors | Developmental cell | 18539114 | Human umbilical cord blood was harvested at Brigham and Women’s Hospital with informed patient consent under an IRB approved protocol. Adult human bone marrow cells were obtained from AllCells, LLC. Mononuclear cells were purified by Ficoll Hypaque s | Full Paper |
2008 | Solomon, DA;Kim, JS;Cronin, JC;Sibenaller, Z;Ryken, T;Rosenberg, SA;Ressom, H;Jean, W;Bigner, D;Yan, H;Samuels, Y;Waldman, T; | Mutational inactivation of PTPRD in glioblastoma multiforme and malignant melanoma | Cancer research | 19074898 | Normal human astrocytes (NHA) were obtained from Clonetics and AllCells. All cell lines were grown in DMEM + 10% fetal bovine serum (FBS) at 37C in 5% CO 2 . Save | Full Paper |
2008 | Lau, YK;Murray, LB;Houshmandi, SS;Xu, Y;Gutmann, DH;Yu, Q; | Merlin is a potent inhibitor of glioma growth | Cancer research | 18632626 | Normal human astrocytes (NHA cells) were obtained from ALLCELLS, Inc. Cells were maintained following the providers' and manufacturers' instructions. Anti-v5 epitope (Invitrogen), Save | Full Paper |
2008 | Solomon, DA;Kim, JS;Jenkins, S;Ressom, H;Huang, M;Coppa, N;Mabanta, L;Bigner, D;Yan, H;Jean, W;Waldman, T; | Identification of p18 INK4c as a tumor suppressor gene in glioblastoma multiforme | Cancer research | 18381405 | Normal human astrocytes (NHAs) were obtained from Clonetics and Allcells. All cell lines were grown in DMEM + 10% fetal bovine serum at 37 in 5% CO 2 . Save | Full Paper |
2008 | Bonini, MG;Stadler, K;Silva, SO;Corbett, J;Dore, M;Petranka, J;Fernandes, DC;Tanaka, LY;Duma, D;Laurindo, FR;Mason, RP; | Constitutive nitric oxide synthase activation is a significant route for nitroglycerin-mediated vasodilation | Proceedings of the National Academy of Sciences of the United States of America | 18562300 | eNOS (Ser-1177 and Thr-495) were from Santa Cruz and used according to the manufacturer's instructions. HUVECs were from Allcells and cultured as described (24). All other chemicals used were analytical grade or superior | Full Paper |
2008 | O'Hare, T;Eide, CA;Tyner, JW;Corbin, AS;Wong, MJ;Buchanan, S;Holme, K;Jessen, KA;Tang, C;Lewis, HA;Romero, RD;Burley, SK;Deininger, MW; | SGX393 inhibits the CML mutant Bcr-AblT315I and preempts in vitro resistance when combined with nilotinib or dasatinib | Proceedings of the National Academy of Sciences of the United States of America | 18367669 | Hematopoietic Colony Forming Assays of Normal Bone Marrow. Bone marrow mononuclear cells (AllCells) from three normal individuals were cultured with SGX393. Cells (5 × 104) were plated in methylcellulose containing 50 ng/ml SCF, 10 ng/ml GM-CSF, and | Full Paper |
2008 | Lund, TC;Tolar, J;Orchard, PJ; | Granulocyte colony-stimulating factor mobilized CFU-F can be found in the peripheral blood but have limited expansion potential | Haematologica | 18403392 | Briefly, blood from G-CSF treated healthy volunteers was purchased from AllCells, LLC (Emeryville, CA, USA) (see Online Supplementary Appendix). The use of all tissue was approved by the Committee on the Use of Human Subjects in Research at the Unive | Full Paper |
2008 | Van Scott, MR;Mertsching, E;Negrou, E;Miles, J;Stallings, HW;Graff, C;Kehry, MR; | Systemic administration of an Fcgamma-Fc(epsilon)-fusion protein in house dust mite sensitive nonhuman primates | Clinical immunology (Orlando, Fla.) | 18583194 | Human basophils were differentiated from CD34+ cord blood progenitors (AllCells, LLC, Emeryville, CA) by 4- to 5-weeks of culture in complete medium [RPMI 1640 (ATCC, Manassas, VA), 10% FBS (HyClone, Logan , UT), 10 μg/ml gentamicin (Sigma-Aldrich, S | Full Paper |
2008 | Reyna, SM;Ghosh, S;Tantiwong, P;Meka, CS;Eagan, P;Jenkinson, CP;Cersosimo, E;Defronzo, RA;Coletta, DK;Sriwijitkamol, A;Musi, N; | Elevated toll-like receptor 4 expression and signaling in muscle from insulin-resistant subjects | Diabetes | 18633101 | e performed Western analysis and real-time PCR of EMR1, a marker for monocyte/macrophage infiltration, in muscle tissue of insulin-resistant subjects and in cultured myotubes. Unlike CD14+ monocytes (AllCells, Emeryville, CA), which expressed EMR1, n | Full Paper |
2008 | Smith, LM;Nesterova, A;Ryan, MC;Duniho, S;Jonas, M;Anderson, M;Zabinski, RF;Sutherland, MK;Gerber, HP;Van Orden, KL;Moore, PA;Ruben, SM;Carter, PJ; | CD133/prominin-1 is a potential therapeutic target for antibody-drug conjugates in hepatocellular and gastric cancers | British journal of cancer | 18542072 | idoma AC133. 1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and normal human primary cells (HREC, hepatocytes) were obtained from Cambrex (Lonza, Switzerland) and AllCells (Emeryville, CA, USA), respectively. Cell | Full Paper |
2008 | Xu, Y;Theobald, V;Sung, C;DePalma, K;Atwater, L;Seiger, K;Perricone, MA;Richards, SM; | Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients | Journal of translational medicine | 18945350 | oulter Immunomics, San Diego, CA). Frozen PBMC Samples: Frozen peripheral blood mononuclear cells (PBMCs), screened HIV negative, were used in this study. PBMC from blood of HLA-A2 healthy subjects (AllCells, LLC, Emeryville, CA and American Red Cro | Full Paper |
2008 | DiBlasio-Smith, EA;Arai, M;Quinet, EM;Evans, MJ;Kornaga, T;Basso, MD;Chen, L;Feingold, I;Halpern, AR;Liu, QY;Nambi, P;Savio, D;Wang, S;Mounts, WM;Isler, JA;Slager, AM;Burczynski, ME;Dorner, AJ;LaVallie, ER; | Discovery and implementation of transcriptional biomarkers of synthetic LXR agonists in peripheral blood cells | Journal of translational medicine | 18925943 | Fresh human PBMC, T cells, B cells, and monocytes from normal human donors were purchased from AllCells (Emeryville, CA). Each cell set was derived from the same donor for Save | Full Paper |
2008 | Inamdar, KV;O'Brien, S;Sen, S;Keating, M;Nguyen, MH;Wang, X;Fernandez, M;Thomazy, V;Medeiros, LJ;Bueso-Ramos, CE; | Aurora-A kinase nuclear expression in chronic lymphocytic leukemia | Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc | 18931650 | fam-ATCAAGATCATTGCTCCTCCTGAGCGC-bhq) at 100 nM concentrations. Real-time quantitative RT-PCR was performed in an automatic MX3000P real-time RT-PCR thermal cycler (Stratagene, La Jolla, CA, USA). Relative RNA level was reported via standard delta del | Full Paper |
2008 | Peterson, SJ;Drummond, G;Kim, DH;Li, M;Kruger, AL;Ikehara, S;Abraham, NG; | L-4F treatment reduces adiposity, increases adiponectin levels, and improves insulin sensitivity in obese mice | Journal of lipid research | 18426778 | Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in α-MEM (Invitrogen) supplemented with 10% heat-inactivated FBS (Invitrogen), and 1% antibiotic/antimycotic solutio | Full Paper |
2008 | Liebler, JM;Lutzko, C;Banfalvi, A;Senadheera, D;Aghamohammadi, N;Crandall, ED;Borok, Z; | Retention of human bone marrow-derived cells in murine lungs following bleomycin-induced lung injury | American journal of physiology. Lung cellular and molecular physiology | 18515407 | Human BMDC preparation and administration. Whole adult human bone marrow cells (Cambrex, Walkersville, MD or AllCells, Emeryville, CA) were used as the source of BMDC under a protocol approved by the Institutional Review Board of the University of So | Full Paper |
2008 | Mina-Osorio, P;Winnicka, B;O'Conor, C;Grant, CL;Vogel, LK;Rodriguez-Pinto, D;Holmes, KV;Ortega, E;Shapiro, LH; | CD13 is a novel mediator of monocytic/endothelial cell adhesion | Journal of leukocyte biology | 18495788 | 37 cells, WEHI‐274.1 (murine myeloid cell line), C33A cells, and hemangioendothelioma (EOMA) cells [19] were from the American Type Cell Culture Collection (ATCC; Manassas, VA, USA). PBMC were from AllCells (Emeryville, CA, USA). Anti‐CD13 mAb 452 an | Full Paper |
2008 | Kopetzki, E;Jekle, A;Ji, C;Rao, E;Zhang, J;Fischer, S;Cammack, N;Sankuratri, S;Heilek, G; | Closing two doors of viral entry: intramolecular combination of a coreceptor- and fusion inhibitor of HIV-1 | Virology journal | 18452606 | s were obtained from the NIH AIDS Research and Reference Reagent program. Human embryonic kidney cells 293-T and HEK293-EBNA cells were obtained from ATCC, Manassas, VA. Human PBMC were obtained from AllCells (Emeryville, CA), stimulated for one day | Full Paper |
2008 | Tomchuck, SL;Zwezdaryk, KJ;Coffelt, SB;Waterman, RS;Danka, ES;Scandurro, AB; | Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responses | Stem cells (Dayton, Ohio) | 17916800 | Human MSCs were obtained from our collaborators at the Tulane University Center for Gene Therapy led by Darwin J. Prockop, M.D., Ph.D. Additionally, MSCs were obtained from two commercial suppliers Cambrex BioScience (Walkersville, MD) and Allcells ( | Full Paper |
2008 | Lee, EJ;Baek, M;Gusev, Y;Brackett, DJ;Nuovo, GJ;Schmittgen, TD; | Systematic evaluation of microRNA processing patterns in tissues, cell lines, and tumors | RNA (New York, N.Y.) | 18025253 | Human cancer cell lines were obtained from ATCC or other investigators. Human total RNA from 20 tissues was from Ambion. CD19+ human B cells were purchased from AllCells. RNA from pancreas and liver tumor and adjacent benign pancreas were obtained fr | Full Paper |
2008 | Clark, PA;Moioli, EK;Sumner, DR;Mao, JJ; | Porous implants as drug delivery vehicles to augment host tissue integration | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 18198217 | Fresh bone marrow samples of multiple adult male donors (AllCells, Berkeley, CA, USA) were used to isolate MSC, per our previous methods (19, 23). Nonadherent cells were removed Save | Full Paper |
2008 | Mittal, R;Prasadarao, NV; | Outer membrane protein A expression in Escherichia coli K1 is required to prevent the maturation of myeloid dendritic cells and the induction of IL-10 and TGF-beta | Journal of immunology (Baltimore, Md. : 1950) | 18684958 | Dendritic cells (DCs) are professional APCs that direct both cellular and humoral immune responses. Escherichia coli K1 causes meningitis in neonates; however, the interactions Save | Full Paper |
2008 | Fukaya, Y;Shimada, H;Wang, LC;Zandi, E;DeClerck, YA; | Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma | The Journal of biological chemistry | 18450743 | transferrin (0.25 μg/ml), selenium (0.25 ng/ml), and l-glutamine. Human BMSC were obtained from ALLCELLS (Emeryville, CA) and maintained in basal medium with stimulatory supplements containing basic fibroblast growth factor (10 ng/ml) | Full Paper |
2008 | Tong, Q;Hirschler-Laszkiewicz, I;Zhang, W;Conrad, K;Neagley, DW;Barber, DL;Cheung, JY;Miller, BA; | TRPC3 is the erythropoietin-regulated calcium channel in human erythroid cells | The Journal of biological chemistry | 18276585 | Human erythroid progenitors/precursors were cultured using a two-phase liquid culture system (31, 32). Cells harvested at day 8 of Phase II were predominantly proerythroblasts and basophilic normoblasts. CD34+ cells were purchased from AllCells, LLC | Full Paper |
2008 | Wang, M;Windgassen, D;Papoutsakis, ET; | Comparative analysis of transcriptional profiling of CD3+, CD4+ and CD8+ T cells identifies novel immune response players in T-cell activation | BMC genomics | 18485203 | ng regulation of T-cell activation in the natural CD3+ population, as well as in the CD4+ and CD8+ subsets. Methods Cells and culture system Healthy-donor peripheral blood mononuclear cells (PBMCs) (AllCells, Berkeley, CA) were used to negatively-se | Full Paper |
2008 | Moioli, EK;Clark, PA;Sumner, DR;Mao, JJ; | Autologous stem cell regeneration in craniosynostosis | Bone | 18023269 | Human mesenchymal stem cells isolated from bone marrow (AllCells, Berkeley, CA) were cultured in 6-well plates (3 × 10 4 cells/well) with osteogenic supplements as described above Save | Full Paper |
2008 | Liang, W;Rahaman, M;Day, D;Marion, N;Riley, G;Mao, J; | Bioactive borate glass scaffold for bone tissue engineering | Journal of Non-Crystalline Solids | Bone marrow derived human mesenchymal stem cells, hbMSCs (AllCells; Berkeley, CA) were cultured for 18 days in either DMEM complete (89% DMEM 10% FBS, 1% penicillin - Save | Full Paper | |
2008 | Sikorska, M;Sandhu, JK;Deb-Rinker, P;Jezierski, A;Leblanc, J;Charlebois, C;Ribecco-Lutkiewicz, M;Bani-Yaghoub, M;Walker, PR; | Epigenetic modifications of SOX2 enhancers, SRR1 and SRR2, correlate with in vitro neural differentiation | Journal of neuroscience research | 18293417 | Primary human astrocytes (HA; prepared from fetuses between 9 and 10 week of gestation) were purchased from AllCells LLC. (Emeryville, CA). The cells were cultured in poly-L-lysine-coated T25-cm2 flasks using basal medium containing stimulatory suppl | Full Paper |
2008 | Papaliodis, D;Boucher, W;Kempuraj, D;Michaelian, M;Wolfberg, A;House, M;Theoharides, TC; | Niacin-induced "flush" involves release of prostaglandin D2 from mast cells and serotonin from platelets: evidence from human cells in vitro and an animal model | The Journal of pharmacology and experimental therapeutics | 18784348 | Freshly isolated human platelets were purchased from AllCells, LLC (Emeryville, CA) and sent to us overnight. Platelets were used the day of arrival after washing as indicated below. Save | Full Paper |
2008 | Kim, DH;Burgess, AP;Li, M;Tsenovoy, PL;Addabbo, F;McClung, JA;Puri, N;Abraham, NG; | Heme oxygenase-mediated increases in adiponectin decrease fat content and inflammatory cytokines tumor necrosis factor-alpha and interleukin-6 in Zucker rats and reduce adipogenesis in human mesenchymal stem cells | The Journal of pharmacology and experimental therapeutics | 18334666 | Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential medium ( Save | Full Paper |
2008 | Fuhrken, PG;Chen, C;Apostolidis, PA;Wang, M;Miller, WM;Papoutsakis, ET; | Gene Ontology-driven transcriptional analysis of CD34+ cell-initiated megakaryocytic cultures identifies new transcriptional regulators of megakaryopoiesis | Physiological genomics | 18252802 | Mk and G cultures were initiated with fresh (AllCells; Berkeley, CA) or frozen (Hematopoietic Cell Processing Core, Fred Hutchinson Cancer Center, Seattle, WA) granulocyte colony-stimulating factor (G-CSF)-mobilized human peripheral blood CD34+ cells | Full Paper |
2008 | Stirewalt, DL;Meshinchi, S;Kopecky, KJ;Fan, W;Pogosova-Agadjanyan, EL;Engel, JH;Cronk, MR;Dorcy, KS;McQuary, AR;Hockenbery, D;Wood, B;Heimfeld, S;Radich, JP; | Identification of genes with abnormal expression changes in acute myeloid leukemia | Genes, chromosomes & cancer | 17910043 | Cancer Research Center (FHCRC) or purchased from commercially available vendors (Cambrex, Rutherford, NJ or AllCells, Emeryville, CA). BM (N = 7) and PB (N = 19) samples from 26 adult AML patients were obtained from the FHCRC's Leukemia Repository. T | Full Paper |
2008 | Li, YY;Bao, M;Meurer, J;Skuballa, W;Bauman, JG;Doecke, WD;Zollner, TM; | The identification of a small molecule inhibitor that specifically reduces T cell-mediated adaptive but not LPS-mediated innate immunity by T cell membrane-monocyte contact bioassay | Immunology letters | 18241931 | PBMCs were either purchased (Allcells, Emeryville, CA) or isolated from healthy donor blood after informed consent. Berlex environmental health & safety (EHS) policies and Save | Full Paper |
2008 | Ramsubir, S;Nonaka, T;Girbés, CB;Carpentier, S;Levade, T;Medin, JA; | In vivo delivery of human acid ceramidase via cord blood transplantation and direct injection of lentivirus as novel treatment approaches for Farber disease | Molecular genetics and metabolism | 18805722 | CD34+ cells were obtained from AllCells, LLC (Berkeley, CA). Cells were pre-stimulated for 12 h in StemSpan SFEM medium (Stem Cell Technologies, Vancouver, BC, Canada) supplemented with recombinant human SCF, Flt3-L, thrombopoietin (all at 50 ng/ml), | Full Paper |
2008 | Huang, D;Pereboev, AV;Korokhov, N;He, R;Larocque, L;Gravel, C;Jaentschke, B;Tocchi, M;Casley, WL;Lemieux, M;Curiel, DT;Chen, W;Li, X; | Significant alterations of biodistribution and immune responses in Balb/c mice administered with adenovirus targeted to CD40(+) cells | Gene therapy | 18046426 | using Adeno-X Rapid Titer kit according to the manufacturer's instructions (BD Biosciences, San Jose, CA, USA). DC culture, Ad infection and luciferase activity assay Myeloid DCs were obtained from AllCELLS, Emeryville, CA, USA. Over 80% of these mo | Full Paper |
2008 | Oh, W;Kim, DS;Yang, YS;Lee, JK; | Immunological properties of umbilical cord blood-derived mesenchymal stromal cells | Cellular immunology | 18495100 | To assess T-cell reactivity against allogeneic cell populations, human responder PBMCs (1 × 105/well) were cocultured with inactivated allogeneic PBMCs (1 × 105/well) or hUCB-MSCs (1 × 102, 1 × 103 or 1 × 104/well) in 96-well tissue culture plates. T | Full Paper |
2008 | Seo, BM;Sonoyama, W;Yamaza, T;Coppe, C;Kikuiri, T;Akiyama, K;Lee, JS;Shi, S; | SHED repair critical-size calvarial defects in mice | Oral diseases | 18938268 | Human bone marrow cells were purchased from commercially available resources (AllCells LLC, Berkeley, CA, USA). To identify putative bone marrow mesenchymal stem cells, single Save | Full Paper |
2008 | Moioli, EK;Clark, PA;Chen, M;Dennis, JE;Erickson, HP;Gerson, SL;Mao, JJ; | Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues | PloS one | 19081793 | Bone marrow cells were isolated from whole marrow aspirates of the iliac crest of each of three healthy male donors (AllCells, Berkeley, CA) and plated as in Fig. 1A. Human Save | Full Paper |
2008 | Yamaza, T;Miura, Y;Bi, Y;Liu, Y;Akiyama, K;Sonoyama, W;Patel, V;Gutkind, S;Young, M;Gronthos, S;Le, A;Wang, CY;Chen, W;Shi, S; | Pharmacologic stem cell based intervention as a new approach to osteoporosis treatment in rodents | PloS one | 18612428 | amethasone (Sigma-Aldrich Co.) were added into the culture for 6 weeks. Human BMMSC culture Human bone marrow aspirates from healthy human adult volunteers (20-35 years of age) were purchased from AllCells LLC and cultured as reported previously [21 | Full Paper |
2008 | Ghosh, A;Park, JY;Fenno, C;Kapila, YL; | Porphyromonas gingivalis, gamma interferon, and a proapoptotic fibronectin matrix form a synergistic trio that induces c-Jun N-terminal kinase 1-mediated nitric oxide generation and cell death | Infection and immunity | 18838522 | Human primary normal peripheral blood neutrophils (AllCells, CA) were maintained in Iscove's modified Dulbecco's medium containing 5% fetal bovine serum, 2 mM glutamine, 25 mM Save | Full Paper |
2008 | Hanten, JA;Vasilakos, JP;Riter, CL;Neys, L;Lipson, KE;Alkan, SS;Birmachu, W; | Comparison of human B cell activation by TLR7 and TLR9 agonists | BMC immunology | 18652679 | 3') was obtained from (Genosys, Woodlands, TX). Isolation and treatment of human peripheral blood B cells Human peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers from AllCells, LLC. (Berkeley, CA) and Memorial Blood Ce | Full Paper |
2008 | López, MC;Lawrence, DA; | Proficiency testing experience for viable CD34+ stem cell analysis | Transfusion | 18298596 | Human peripheral blood was collected in blood collection tubes (Cyto-Chex BCT, Streck Laboratories, La Vista, NE), which contain a preservative reagent for flow cytometry. Frozen CD34+ stem cells from cord blood (AllCells LLC, Berkeley, CA) were thaw | Full Paper |
2008 | Tang, H;Lu, JY;Zheng, X;Yang, Y;Reagan, JD; | The psoriasis drug monomethylfumarate is a potent nicotinic acid receptor agonist | Biochemical and biophysical research communications | 18722346 | 2B were purchased from AllCells (Emeryville, CA) and were of greater than 95% purity. Human CD14+ monocytes and NHEK were from Cambrex (East Rutherford, NJ) and cultured as Save | Full Paper |
2008 | Bachstetter, AD;Pabon, MM;Cole, MJ;Hudson, CE;Sanberg, PR;Willing, AE;Bickford, PC;Gemma, C; | Peripheral injection of human umbilical cord blood stimulates neurogenesis in the aged rat brain | BMC neuroscience | 18275610 | cord blood mononuclear cells (UCBMCs) were obtained from Saneron CCEL Therapeutics, Inc. (Tampa, FL, USA). Cryopreserved Human peripheral blood cells (PBMC) (mononuclear fraction) were obtained from AllCells, LLC (Emeryville, CA, USA). Just prior to | Full Paper |
2008 | Mashiyama, ST;Hansen, CM;Roitman, E;Sarmiento, S;Leklem, JE;Shultz, TD;Ames, BN; | An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiency | Analytical biochemistry | 17963712 | , respectively, in measuring DNA-uracil in all the samples used in experiments described in this paper (lymphocytes from the B6 deficiency study and lymphocytes from AllCells). Uracil Save | Full Paper |
2008 | Park, EJ;Kim, ES;Weber, HP;Wright, RF;Mooney, DJ; | Improved bone healing by angiogenic factor-enriched platelet-rich plasma and its synergistic enhancement by bone morphogenetic protein-2 | The International journal of oral & maxillofacial implants | 19014150 | Fresh human adult whole blood was purchased from AllCells (Emeryville, CA, USA). Peripheral blood mononuclear cells (PBMNCs) and platelets were separately isolated by a density gradient centrifugation (Histopaque-1077, Sigma, St. Louis, MO, USA) acco | Full Paper |
2008 | Thamm, DH;O'Brien, MG;Vail, DM; | Serum vascular endothelial growth factor concentrations and postsurgical outcome in dogs with osteosarcoma | Veterinary and comparative oncology | 19178672 | Vascular endothelial growth factor (VEGF) is a key angiogenic growth factor, playing putative roles in both tumour growth and metastasis. The purpose of this study was to correlate Save | Full Paper |
2008 | Antonescu, CR;Wu, K;Xing, GL;Cao, M;Turpaz, Y;Leversha, MA;Hubbell, E;Maki, RG;Miyada, CG;Pillai, R; | DNA copy number analysis in gastrointestinal stromal tumors using gene expression microarrays | Cancer informatics | 19259404 | from the American Type Culture Collection (ATCC, Manassas, VA). DNA was extracted from the cultured cells using the DNA Maxi Kit (Qiagen, Inc., Valencia, CA). DNA from normal blood was obtained from AllCells, LLC (Emeryville, CA). GIST sample DNA was | Full Paper |
2008 | Li, M;Rossi, JJ; | Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells | Methods in molecular biology (Clifton, N.J.) | 18679631 | Target cells: K562 cells, a chronic myelogenous leukemia cell line (ATCC, #CCL-243). CD34+ hematopoietic stem cells, purchased from AllCells (Berkley, CA, USA) or purified from umbilical cord blood or bone marrow by anti-CD34 antibody-coupled magneti | Full Paper |
2008 | Campbell-Anson, RE;Kentor, D;Wang, YJ;Bushnell, KM;Li, Y;Vence, LM;Radvanyi, LG; | A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology | Journal of immune based therapies and vaccines | 18321390 | Sources of PBMC for DC generation PBMC were obtained from peripheral blood leukopheresis products obtained from non-mobilized normal donors (LifeBlood, Memphis, TN), or G-CSF-mobilized normal donors (AllCells, Berkeley, CA). Products were collected i | Full Paper |
2008 | Tiainen, J; | Structured equity products and portfolio compositions of financially advised investors | Thesis | RESULTS I will show in this thesis that behavioural biases of the retail investors are associated to proportions of portfolio invested in structured capital guaranteed products and in equity Save | Full Paper | |
2008 | Shi, M; | The Role of Janus-Kinase-3 in CD4+ T Cell Proliferation and Differentiation: A Dissertation | Thesis | Aliquots of cryopreserved peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryville, CA). PBMC were resuspended in complete RPMI 1640 medium (RPMI 1640 medium supplemented with 10% fetal bovine serum, penicillin/streptomycin, | Full Paper | |
2008 | Ziegler, LA; | Targeting lentivector gene delivery to hematopoietic stem and progenitor cells in vivo | Thesis | Fresh, unfractionated human PBMCs (2 x 106 ) (AllCells) were incubated with concentrated virus with total TUs of 10 x 106 (based on the titer on 293T/CD20 cells). LPS (50 μg/ml) was then added for B cells to survive and grow. After 2 days, cells wer | Full Paper | |
2008 | Wang, M; | Genomic-scale transcriptional analysis of T-cell activation reveals novel genes and signaling programs | Thesis | Healthy-donor peripheral blood mononuclear cells (PBMCs) (AllCells, Berkeley, CA) were used to negatively-select CD3+ T cells, CD4+ and CD8+ T cells (Pan T Cell Isolation Kit II, CD4+ T Cell Isolation Kit II, and CD8+ T Cell Isolation Kit II, Milteny | Full Paper | |
2008 | Bedi, R; | Characterization of the activities of the human C/EBPϵ activator and repressor isoforms in myelopoiesis | Thesis | 25 ml/cm2 . After preloading and before prestimulation, the plates were washed with PBS to remove any inhibitory molecules in the viral supernatant. Cord blood CD34+ cells purchased from AllCells (CA, USA) were thawed according to the supplier's inst | Full Paper | |
2007 | Aziz, MH;Manoharan, HT;Church, DR;Dreckschmidt, NE;Zhong, W;Oberley, TD;Wilding, G;Verma, AK; | Protein kinase Cepsilon interacts with signal transducers and activators of transcription 3 (Stat3), phosphorylates Stat3Ser727, and regulates its constitutive activation in prostate cancer | Cancer Res. | 17875724 | Prostate cancer specimens were obtained at radical prostatectomy. The University of Wisconsin Human Subjects Committee approved the use of these human prostate specimens. Tissue microarrays were obtained from Folio Biosciences.4 Tissue microarrays we | Full Paper |
2007 | Senzer, N;Nemunaitis, J;Nemunaitis, M;Lamont, J;Gore, M;Gabra, H;Eeles, R;Sodha, N;Lynch, FJ;Zumstein, LA;Menander, KB;Sobol, RE;Chada, S; | p53 therapy in a patient with Li-Fraumeni syndrome | Mol. Cancer Ther. | 17483435 | Full Paper | |
2007 | Gagna, CE;Clark Lambert, W; | Cell biology, chemogenomics and chemoproteomics - application to drug discovery | Expert Opin Drug Discov | 23484648 | TMAs have been expensive and limited in terms of commercial availability [8,56-59]. Only recently have they surfaced commercially on a large scale (e.g., Cytomyx, Folio Biosciences, Chemicon). Many different types of high quality TMAs now exist, such | Full Paper |
2007 | Flores, J;Cepeda, IL;Cornfeldt, ML;O'Kusky, JR;Doudet, DJ; | Characterization and survival of long-term implants of human retinal pigment epithelial cells attached to gelatin microcarriers in a model of Parkinson disease | J. Neuropathol. Exp. Neurol. | 17620984 | All primary antibodies were previously assessed for hRPE cell specificity in human RPE cultured monolayer and RPE-choroid eye tissue, and for nonspecificity to striatal rat tissue (Dr. S. Bernstein, QualTek Molecular Laboratories, Santa Barbara, CA, | Full Paper |
2007 | Lynch, FC; | Use of a covered stent for percutaneous transrenal occlusion of the ureter | J Vasc Interv Radiol | 18004001 | Full Paper | |
2007 | Cantwell, CP;Lynch, FC; | Caudal migration of a G2 filter during carbon dioxide cavography | J Vasc Interv Radiol | 17538152 | Full Paper | |
2007 | Lee, S;Sha, Q;Wu, X;Calenda, G;Peng, J; | Expression profiles of mouse Kell, XK, and XPLAC mRNA | J. Histochem. Cytochem. | 17189525 | For the ISH experiments, the custom service from Phylogeny Inc. (Columbus, OH) was used (Lyons et al. 1990; Patapoutian et al. 1993). Briefly, tissue sections were hybridized overnight at 55C in 50% deionized formamide, 0.3 M NaCl, 20 mM Tris-HCl, pH | Full Paper |
2007 | Tanimoto, R;Abarzua, F;Sakaguchi, M;Takaishi, M;Nasu, Y;Kumon, H;Huh, NH; | REIC/Dkk-3 as a potential gene therapeutic agent against human testicular cancer | Int. J. Mol. Med. | 17273781 | Tissues, cells and reagents. A testicular carcinoma tissue microarray was purchased from Folio Biosciences (Columbus, OH). Surgically resected testicular cancer tissues were fixed with 4% paraformaldehyde and tissue sections were made under conventio | Full Paper |
2007 | Calin, GA;Liu, CG;Ferracin, M;Hyslop, T;Spizzo, R;Sevignani, C;Fabbri, M;Cimmino, A;Lee, EJ;Wojcik, SE;Shimizu, M;Tili, E;Rossi, S;Taccioli, C;Pichiorri, F;Liu, X;Zupo, S;Herlea, V;Gramantieri, L;Lanza, G;Alder, H;Rassenti, L;Volinia, S;Schmittgen, TD;Kipps, TJ;Negrini, M;Croce, CM; | Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomas | Cancer cell | 17785203 | An additional set of 3 normal CD19+/CD5+ positive B cells, not used for microarray studies, was purchased from AllCells (Berkeley, CA), and used as an independent confirmation set. In all instances the qRT-PCR data confirmed the microarray data. RNA | Full Paper |
2007 | Wang, L;Wang, J;Blaser, BW;Duchemin, AM;Kusewitt, DF;Liu, T;Caligiuri, MA;Briesewitz, R; | Pharmacologic inhibition of CDK4/6: mechanistic evidence for selective activity or acquired resistance in acute myeloid leukemia | Blood | 17537993 | Entry into the cell cycle is mediated by cyclin-dependent kinase 4/6 (CDK4/6) activation, followed by CDK2 activation. We found that pharmacologic inhibition of the Flt3 internal tandem Save | Full Paper |
2007 | Mulligan, G;Mitsiades, C;Bryant, B;Zhan, F;Chng, WJ;Roels, S;Koenig, E;Fergus, A;Huang, Y;Richardson, P;Trepicchio, WL;Broyl, A;Sonneveld, P;Shaughnessy, JD;Bergsagel, PL;Schenkein, D;Esseltine, DL;Boral, A;Anderson, KC; | Gene expression profiling and correlation with outcome in clinical trials of the proteasome inhibitor bortezomib | Blood | 17185464 | The aims of this study were to assess the feasibility of prospective pharmacogenomics research in multicenter international clinical trials of bortezomib in multiple myeloma and to Save | Full Paper |
2007 | Brogdon, JL;Xu, Y;Szabo, SJ;An, S;Buxton, F;Cohen, D;Huang, Q; | Histone deacetylase activities are required for innate immune cell control of Th1 but not Th2 effector cell function | Blood | 17008546 | as described previously.22,23 Human neutrophils were obtained from AllCells (Berkeley, CA). Mouse bone marrow-derived DCs were prepared as described previously.24 Murine Th1 and Th2 effector cells were differentiated from DO11.10 mice as described | Full Paper |
2007 | Mahadevan, D;Klinkhammer, T;Cooke, L;Riley, C;Choi, J;Sinari, S;Miller, T;Spier, C; | CLL Transcript and Serum Profiling Identifies Distinct Oncogenic Pathways in Rai Stage Progression. | Blood | Full Paper | ||
2007 | Robinson, S;Simmons, P;Brouard, N;Kidd, S;Yang, H;Decker, W;Xing, D;Steiner, D;Ng, J;Thomas, M;Champlin, R;de Lima, M;Hosing, C;Cooper, L;Singh, H;Zweidler-McKay, P;Shpall, E; | Efficacy of ‘Off-the-Shelf’, Commercially-Available, Third-Party Mesenchymal Stem Cells (MSC) in Ex Vivo Cord Blood (CB) Co-Culture Expansion. | Blood | INTRODUCTION: Our previous studies have shown that clinically-relevant levels of hematopoietic stem and progenitor cell (HSPC) expansion are possible by ex vivo co-culture of cord blood (CB) mononuclear cells (MNC) with third-party bone marrow (BM)-d | Full Paper | |
2007 | Majeti, R;Park, CY;Weissman, IL; | Identification of a hierarchy of multipotent hematopoietic progenitors in human cord blood | Cell stem cell | 18371405 | Normal human bone marrow mononuclear cells were purchased from AllCells Inc. (Emeryville, CA). Human cord blood was collected from placentas and/or umbilical cords obtained Save | Full Paper |
2007 | Scholl, C;Bansal, D;Döhner, K;Eiwen, K;Huntly, BJ;Lee, BH;Rücker, FG;Schlenk, RF;Bullinger, L;Döhner, H;Gilliland, DG;Fröhling, S; | The homeobox gene CDX2 is aberrantly expressed in most cases of acute myeloid leukemia and promotes leukemogenesis | The Journal of clinical investigation | 17347684 | and all patients and volunteers gave informed consent according to the Declaration of Helsinki. Seven of the 10 normal BMMC samples and the 3 normal BM samples that were used for isolating HSCs, CMPs, GMPs, and MEPs were purchased from AllCells | Full Paper |
2007 | Xin, X;Hussain, M;Mao, JJ; | Continuing differentiation of human mesenchymal stem cells and induced chondrogenic and osteogenic lineages in electrospun PLGA nanofiber scaffold | Biomaterials | 17010425 | Bone-marrow-derived hMSCs were purchased from AllCells (Berkeley, CA, USA). The donor was a 25 year-old healthy male volunteer without additional identifier. In our previous work, we have isolated hMSCs from multiple donors [5]. In this report, the i | Full Paper |
2007 | Zou, H;Harrington, JJ;Sugumar, A;Klatt, KK;Smyrk, TC;Ahlquist, DA; | Detection of colorectal disease by stool defensin assay: an exploratory study | Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association | 17531545 | These cDNA samples and a purchased neutrophil cNDA (AllCells, Emeryville, CA) were amplified by real-time polymerase chain reaction (PCR) in an iCycler (Bio-Rad, Hercules, CA). Save | Full Paper |
2007 | Zhao, Y;Parkhurst, MR;Zheng, Z;Cohen, CJ;Riley, JP;Gattinoni, L;Restifo, NP;Rosenberg, SA;Morgan, RA; | Extrathymic generation of tumor-specific T cells from genetically engineered human hematopoietic stem cells via Notch signaling | Cancer research | 17363559 | CD133 + progenitor cells were enriched from mononuclear cells from UCB (Allcells, Berkeley, CA) by depleting CD3 + cells using Dynabeads human CD3 (Invitrogen, Carlsbad, CA) Save | Full Paper |
2007 | Verhelle, D;Corral, LG;Wong, K;Mueller, JH;Moutouh-de Parseval, L;Jensen-Pergakes, K;Schafer, PH;Chen, R;Glezer, E;Ferguson, GD;Lopez-Girona, A;Muller, GW;Brady, HA;Chan, KW; | Lenalidomide and CC-4047 inhibit the proliferation of malignant B cells while expanding normal CD34+ progenitor cells | Cancer research | 17234786 | Cell culture. Namalwa CSN.70 and LP-1 cells were purchased from DSMZ (Brauschweig, Germany), U266 was purchased from the American Type Culture Collection (Manassas, VA), and cord blood CD34+ cells were purchased from AllCells (Emeryville, CA). Namalw | Full Paper |
2007 | Pariente, N;Morizono, K;Virk, MS;Petrigliano, FA;Reiter, RE;Lieberman, JR;Chen, IS; | A novel dual-targeted lentiviral vector leads to specific transduction of prostate cancer bone metastases in vivo after systemic administration | Molecular therapy : the journal of the American Society of Gene Therapy | 17653099 | endothelial cells were obtained from AllCells (Emeryville, CA) and grown in human umbilical vein endothelial cell basal medium plus stimulatory supplement (AllCells, Emelyville, CA). Save | Full Paper |
2007 | Sonoyama, W;Seo, BM;Yamaza, T;Shi, S; | Human Hertwig's epithelial root sheath cells play crucial roles in cementum formation | Journal of dental research | 17586703 | Human bone marrow mesenchymal stem cells (BMMSCs) were purchased from a commercially available resource (AllCells LLC, Berkeley, CA, USA), and cultured as previously described (Batouli et al., 2003). Cemento/osteogenic differentiation was induced and | Full Paper |
2007 | Li, L;Piloto, O;Kim, KT;Ye, Z;Nguyen, HB;Yu, X;Levis, M;Cheng, L;Small, D; | FLT3/ITD expression increases expansion, survival and entry into cell cycle of human haematopoietic stem/progenitor cells | British journal of haematology | 17359372 | Activating mutation of FLT3 by internal tandem duplications (ITDs) in the juxtamembrane region is the most common molecular aberration found in acute myeloid leukaemia (AML). In Save | Full Paper |
2007 | Kikuchi, J;Shimizu, R;Wada, T;Ando, H;Nakamura, M;Ozawa, K;Furukawa, Y; | E2F-6 suppresses growth-associated apoptosis of human hematopoietic progenitor cells by counteracting proapoptotic activity of E2F-1 | Stem cells (Dayton, Ohio) | 17600109 | magnetic cell sorting; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com) from human bone marrow mononuclear cells (BM-MNCs) (purchased from AllCells, Berkeley, CA, http://www.allcells.com) or cord blood mononuclear cells (CB | Full Paper |
2007 | Telford, WG;Bradford, J;Godfrey, W;Robey, RW;Bates, SE; | Side population analysis using a violet-excited cell-permeable DNA binding dye | Stem cells (Dayton, Ohio) | 17185610 | (FBS)/10% dimethyl sulfoxide in liquid nitrogen until use. Frozen human cord blood was obtained from AllCells LLC (Emeryville, CA, http://www.allcells.com) and thawed immediately before use. A549 lung carcinoma cells were grown in Dulbecco's modified | Full Paper |
2007 | Fang, D;Seo, BM;Liu, Y;Sonoyama, W;Yamaza, T;Zhang, C;Wang, S;Shi, S; | Transplantation of mesenchymal stem cells is an optimal approach for plastic surgery | Stem cells (Dayton, Ohio) | 17170063 | Human bone marrow cells were purchased from commercially available resources (AllCells LLC, Berkeley, CA,http://www.allcells.com/). To identify putative bone marrow MSCs, single-cell suspension of 1 × 106 of bone marrow mononuclear cells were seeded | Full Paper |
2007 | Stosich, MS;Mao, JJ; | Adipose tissue engineering from human adult stem cells: clinical implications in plastic and reconstructive surgery | Plastic and reconstructive surgery | 17255658 | Human mesenchymal stem cells were isolated from fresh bone marrow samples of an anonymous adult male donor (AllCells, Berkeley, Calif.) by following our previous methods. After Save | Full Paper |
2007 | Kim, JS;Lee, C;Bonifant, CL;Ressom, H;Waldman, T; | Activation of p53-dependent growth suppression in human cells by mutations in PTEN or PIK3CA | Molecular and cellular biology | 17060456 | were described in a previous study (27). BJ-hTERT and RPE-hTERT cells were obtained from Clontech (Mountain View, CA). Normal human astrocytes (NHA) were obtained from AllCells LLC (Emeryville, CA) and cultured as recommended. HEK293, MCF10A, and A17 | Full Paper |
2007 | Chen, C;Fuhrken, PG;Huang, LT;Apostolidis, P;Wang, M;Paredes, CJ;Miller, WM;Papoutsakis, ET; | A systems-biology analysis of isogenic megakaryocytic and granulocytic cultures identifies new molecular components of megakaryocytic apoptosis | BMC genomics | 17953764 | tudies of individual candidate genes. Methods Hematopoietic cultures Mk and G cell cultures were initiated with fresh or frozen G-CSF-mobilized human peripheral blood CD34+ cells from normal donors (AllCells; Berkeley, CA) and cultured as described | Full Paper |
2007 | Vose, SC;Holland, NT;Eskenazi, B;Casida, JE; | Lysophosphatidylcholine hydrolases of human erythrocytes, lymphocytes, and brain: sensitive targets of conserved specificity for organophosphorus delayed neurotoxicants | Toxicology and applied pharmacology | 17663017 | Whole human blood from seven subjects and lymphocytes (isolated by aphaeresis using a leukopac filter) from three subjects were obtained from AllCells LLC (Emeryville, CA). Mouse Save | Full Paper |
2007 | Zeng, Z;Yuan, X;Liu, G;Zeng, X;Zeng, X;Ng, H;Chen, H;Jiang, T;Akasaki, Y;Kessey, K;Black, KL;Yu, JS; | Manipulation of proliferation and differentiation of human bone marrow-derived neural stem cells in vitro and in vivo | Journal of neuroscience research | 17131390 | Human bone marrow cells (HBMC) were isolated from fresh human whole bone marrow (AllCells, Berkely, CA) by gradient density centrifugation (Lee et al., 2003). HBMC were plated at a density of 2 × 106 cells/ml/well onto 24-well plates (Corning, Cornin | Full Paper |
2007 | Huang, LT;Paredes, CJ;Papoutsakis, ET;Miller, WM; | Gene expression analysis illuminates the transcriptional programs underlying the functional activity of ex vivo-expanded granulocytes | Physiological genomics | 17550995 | of PB granulocytes should reflect their full activation potential, because RNA levels would not be expected to change very much during cell transportation and processing by AllCells ( | Full Paper |
2007 | Uno, S;Kinoshita, Y;Azuma, Y;Tsunenari, T;Yoshimura, Y;Iida, S;Kikuchi, Y;Yamada-Okabe, H;Fukushima, N; | Antitumor activity of a monoclonal antibody against CD47 in xenograft models of human leukemia | Oncology reports | 17390064 | The ligation of CD47 induces the apoptosis of leukemic cells in a caspase-independent manner. We generated a monoclonal antibody against CD47 (mAb-MABL) that possibly induced Save | Full Paper |
2007 | Jenkins, J;Calzone, KA; | Establishing the essential nursing competencies for genetics and genomics | Journal of nursing scholarship : an official publication of Sigma Theta Tau International Honor Society of Nursing | 17393960 | 129This annotation involves adding the units and labels to allcells containing no references to other cells. The systemthen analyzes the cells with formulas containing referencesand Save | Full Paper |
2007 | Furuhata, S;Ando, K;Oki, M;Aoki, K;Ohnishi, S;Aoyagi, K;Sasaki, H;Sakamoto, H;Yoshida, T;Ohnami, S; | Gene expression profiles of endothelial progenitor cells by oligonucleotide microarray analysis | Molecular and cellular biochemistry | 17203242 | Among the many tissue stem or progenitor cells recently being unveiled, endothelial progenitor cells (EPCs) have attracted particular attention, not only because of their cardinal role in Save | Full Paper |
2007 | Yourek, G;Hussain, MA;Mao, JJ; | Cytoskeletal changes of mesenchymal stem cells during differentiation | ASAIO journal (American Society for Artificial Internal Organs : 1992) | 17413564 | Human mesenchymal stem cells were isolated from commercially available whole bone marrow (AllCells, Berkeley, CA) using RosetteSep (StemCell Technologies, Vancouver, BC) according to the manufacturer’s instructions. The RosetteSep antibody cocktail c | Full Paper |
2007 | Baysal, BE; | A recurrent stop-codon mutation in succinate dehydrogenase subunit B gene in normal peripheral blood and childhood T-cell acute leukemia | PloS one | 17487275 | 80°C. cDNAs synthesized from column-purified normal PBMC cell-type subsets, including monocytes (CD14+), NK cells (CD56+, negative selection), B (CD19+) and T lymphocytes (CD3+), were purchased from AllCells, LLC Inc. through StemCell Technologies. E | Full Paper |
2007 | Stosich, MS;Bastian, B;Marion, NW;Clark, PA;Reilly, G;Mao, JJ; | Vascularized adipose tissue grafts from human mesenchymal stem cells with bioactive cues and microchannel conduits | Tissue engineering | 17824832 | Human MSCs were isolated from fresh bone marrow samples of two anonymous adult donors (AllCells, Berkeley, CA), per our previous methods.After transferring bone marrow sample Save | Full Paper |
2007 | Popov, BV;Serikov, VB;Petrov, NS;Izusova, TV;Gupta, N;Matthay, MA; | Lung epithelial cells induce endodermal differentiation in mouse mesenchymal bone marrow stem cells by paracrine mechanism | Tissue engineering | 17630877 | Human hematopoietic stem cells (CD34þ) were purchased from AllCells Co. (Berkeley, CA) and confirmed to be 96% pure using FACScan. Cells were washed twice in PBS, labeled with PKH26 (Sigma) according to manufacturer’s instructions, and mixed with epi | Full Paper |
2007 | Moioli, EK;Hong, L;Mao, JJ; | Inhibition of osteogenic differentiation of human mesenchymal stem cells | Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society | 17537129 | hMSCs were isolated from donated human bone marrow samples (AllCells, Berkeley, CA) and plated in monolayers in six-well plates at a density of 3×104 cells/well per our previous methods36 (Figure 1A and B). The isolated hMSCs were cultured-expanded a | Full Paper |
2007 | Anderson, RM;Stevens, DL;Sumption, ND;Townsend, KM;Goodhead, DT;Hill, MA; | Effect of linear energy transfer (LET) on the complexity of alpha-particle-induced chromosome aberrations in human CD34+ cells | Radiation research | 17474795 | Frozen human bone marrow CD34 + cells were obtained commercially (Poietics, Cambrex and AllCells, LLC) in vials of 1 × 10 6 cells (samples are anonymized and exempt from Save | Full Paper |
2007 | Sawyer, L;Hanson, D;Castro, G;Luckett, W;Dubensky, TW;Stassinopoulos, A; | Inactivation of parvovirus B19 in human platelet concentrates by treatment with amotosalen and ultraviolet A illumination | Transfusion | 17524098 | CD34+ cells isolated from granulocyte-colony-stimulating factor-mobilized human peripheral blood were obtained from AllCells (Berkeley, CA) and stimulated to proliferate and Save | Full Paper |
2007 | Wang, TT;Tio, M;Lee, W;Beerheide, W;Udolph, G; | Neural differentiation of mesenchymal-like stem cells from cord blood is mediated by PKA | Biochemical and biophysical research communications | 17466951 | Cell culture and growth profiling. MNCs (AllCells) were seeded in MyeloCult medium (Stem Cell Technologies) supplemented with 1 × 10−7 M dexamethasone (Sigma). Adherent cells were trypsinized (0.125% Trypsin-EDTA) and passaged at near-confluency (70- | Full Paper |
2007 | Chen, L;Gao, Z;Zhu, J;Rodgers, GP; | Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrow | Experimental hematology | 17588473 | Human bone marrow CD133+ cells (AllCells, Berkeley, CA, USA) were cultured for 14-28 days in the presence of EPO or G-CSF. All cells were grown in media that included stem cell factor (SCF, 50 ng/mL), interleukin-3 (IL-3, 10 ng/mL), granulocyte-macro | Full Paper |
2007 | Chua, KN;Chai, C;Lee, PC;Ramakrishna, S;Leong, KW;Mao, HQ; | Functional nanofiber scaffolds with different spacers modulate adhesion and expansion of cryopreserved umbilical cord blood hematopoietic stem/progenitor cells | Experimental hematology | 17577926 | Frozen human umbilical cord blood CD34+ HSPCs were purchased from AllCells, LLC (Emeryville, CA, USA). The CD34+ purity in the post-thawed HSPC was determined to be 98% by flow cytometry and the viability was determined to be more than 97% by Trypan | Full Paper |
2007 | Zwezdaryk, KJ;Coffelt, SB;Figueroa, YG;Liu, J;Phinney, DG;LaMarca, HL;Florez, L;Morris, CB;Hoyle, GW;Scandurro, AB; | Erythropoietin, a hypoxia-regulated factor, elicits a pro-angiogenic program in human mesenchymal stem cells | Experimental hematology | 17379074 | Cambrex BioScience (Walkersville, MD, USA) and Allcells (Emeryville, CA, USA), to ensure enough variability of the starting cell population and make certain that findings are universal and not unique to single donor pools derived from a unique source | Full Paper |
2007 | Cheng, X;Macvittie, T;Meisenberg, B;Welty, E;Farese, A;Tadaki, D;Takebe, N; | Human brain endothelial cells (HUBEC) promote SCID repopulating cell expansion through direct contact | Growth factors (Chur, Switzerland) | 18049950 | The objective of this study was to re-evaluate the previously published hematopoietic stem cell (HSC) expansion work using human brain endothelial cells (HUBEC). The expansion Save | Full Paper |
2007 | Basiji, DA;Ortyn, WE;Liang, L;Venkatachalam, V;Morrissey, P; | Cellular image analysis and imaging by flow cytometry | Clinics in laboratory medicine | 17658411 | To investigate the utility of imaging flow cytometry for clinical FISH analysis, human peripheral blood mononuclear cells (PBMC) were obtained (AllCells, San Diego, CA) and probed using a FISH in suspension (FISHIS®) protocol developed at Amnis Corpo | Full Paper |
2007 | King, JA; | Cell culture systems inspired by the hematopoietic stem cell niche | Thesis | were maintained between 0.5 and 1x106 cells/mL. 3.3.5 Culture of CD34+ selected cells CD34+ selected cells were purchased from AllCells (Berkeley, CA). AllCells obtained informed consent from volunteers before donors provided cells | Full Paper | |
2007 | Bell, A;Meyer-Ebrecht, D;Bocking, A;Aach, T; | HDR-Microscopy of Cell Specimens: Imaging and Image Analysis | 2007 Conference Record of the Forty-First Asilomar Conference on Signals, Systems and Computers | The counterstain affects allcells, ie, independent of the protein which is stained by the immunocytochemical marker. Hence, marker positive cells will be affected by both stains at once. Save | Full Paper | |
2007 | 이연경, ; | Effects of cationic liposomes on in vitro and in vivo transduction mediated by adenoviral vectors | Thesis | According to theresultofGFP expression in HeLa cells(Figure 3A),nearly allcells were transduced in the presence of the 100 MOIof Ad-vectors. Meanwhile, CAR-deficient B16BL6 cells Save | Full Paper | |
2007 | NGIAP, CK; | Bio-functionalization of Electrospun Nanofibre Scaffolds for Cell Culture Applications | Thesis | Frozen human umbilical cord blood CD34+ HSPCs were purchased from AllCells, which were obtained from normal volunteers participating in an Institute Reviewing Board (IRB) approved donor program (AllCells). The CD34+ purity in the HSPC was determine | Full Paper | |
2007 | Giammona, LM; | Nicotinamide enhances primary human megakaryocytic differentiation from hematopoietic stem cells: Phenotypic characterization and mechanism of action | Thesis | Unless otherwise noted, all reagents were obtained from Sigma-Aldrich (St. Louis, MO). Cultures were initiated with previously frozen human mobilized peripheral blood CD34+ cells (AllCells; Berkeley, CA). Cells were cultured in T-flasks and maintaine | Full Paper | |
2007 | Troken, AJ; | Mesenchymal stem cells and derivatives in hydrogels | Thesis | HMSCs were isolated from commercially available healthy human bone marrow (AllCells, LLC; Berkeley, CA) using RosetteSep kit (StemCell Technologies, Inc., Vancouver, BC, Canada). Bone marrow extracts contain heterogeneous cell populations. MSCs repre | Full Paper | |
2006 | Perk, J;Gil-Bazo, I;Chin, Y;de Candia, P;Chen, JJ;Zhao, Y;Chao, S;Cheong, W;Ke, Y;Al-Ahmadie, H;Gerald, WL;Brogi, E;Benezra, R; | Reassessment of id1 protein expression in human mammary, prostate, and bladder cancers using a monospecific rabbit monoclonal anti-id1 antibody | Cancer Res. | 17108123 | Two slides of commercially available (Folio Biosciences, Columbus, OH) TMAs were also analyzed. The two slides included a total of 143 cores, of which 62 samples were common types of breast cancer, and the rest of the samples were benign (slides #ARY | Full Paper |
2006 | Swales, KE;Korbonits, M;Carpenter, R;Walsh, DT;Warner, TD;Bishop-Bailey, D; | The farnesoid X receptor is expressed in breast cancer and regulates apoptosis and aromatase expression | Cancer Res. | 17047076 | Samples of human infiltrating ductal breast carcinoma and near tumor normal samples were obtained from 10 patients after receiving ethical approval and individual patient consent. Protein was extracted using Cytobuster (Novagen, Merck Biosciences, No | Full Paper |
2006 | Evans, EE;Henn, AD;Jonason, A;Paris, MJ;Schiffhauer, LM;Borrello, MA;Smith, ES;Sahasrabudhe, DM;Zauderer, M; | C35 (C17orf37) is a novel tumor biomarker abundantly expressed in breast cancer | Mol. Cancer Ther. | 17121940 | Staining of formalin-fixed, paraffin-embedded breast carcinoma and normal tissue sections was done in part by QualTek Molecular Labs (Santa Barbara, CA). Ten lobular and 50 invasive ductal (grades 1, 2, and 3) carcinomas were selected from the QualTe | Full Paper |
2006 | Roth, JD;Hughes, H;Kendall, E;Baron, AD;Anderson, CM; | Antiobesity effects of the beta-cell hormone amylin in diet-induced obese rats: effects on food intake, body weight, composition, energy expenditure, and gene expression | Endocrinology | 16935845 | To compare central nervous system gene expression across amylin- and pair-fed-treated rats that had exhibited a similar magnitude of weight loss, four animals from each group were selected for ISH, counterbalanced for their final body weight on the d | Full Paper |
2006 | Couse, JF;Yates, MM;Rodriguez, KF;Johnson, JA;Poirier, D;Korach, KS; | The intraovarian actions of estrogen receptor-alpha are necessary to repress the formation of morphological and functional Leydig-like cells in the female gonad | Endocrinology | 16627580 | ... }X56974 [/nuccore/X56974] 1271–1351 GAAACTGCGAATGGCTCATTAA GAATTACCACAGTTATCCAAGTAGGA Open in a separate window [/pmc/articles/PMC1892221/table/T1/?report=objectonly] IN SITU HYBRIDIZATION Phylogeny Inc. (Columbus, OH) conducted all _in | Full Paper |
2006 | Costello, C;Ward, M; | Search, bioprospecting and biodiversity conservation | Journal of Environmental Economics and Management | in a particular way. When executed in bulk, these tests cost between $4000 and $18,000 per species tested (eg Qualtek Molecular Laboratories, Santa Barbara, CA). We use a range for c of $4000-$18,000. • Discount rate (r | Full Paper | |
2006 | Cantwell, CP;Lynch, FC; | Ureterocutaneous fistula and urostomy exclusion with use of a covered wallstent | J Vasc Interv Radiol | 16614159 | Full Paper | |
2006 | Kutty, RK;Chen, S;Samuel, W;Vijayasarathy, C;Duncan, T;Tsai, JY;Fariss, RN;Carper, D;Jaworski, C;Wiggert, B; | Cell density-dependent nuclear/cytoplasmic localization of NORPEG (RAI14) protein | Biochem. Biophys. Res. Commun. | 16729964 | Immunohistochemistry. Immunohistochemistry was performed by QualTek Molecular Laboratories. Briefly, four-micron human placenta sections were deparaffinized, subjected to 20 min of steam heat induced epitope recovery, and then treated with anti-NORPE | Full Paper |
2006 | Cantwell, C;Waybill, P;Lynch, F;Taubman, K; | Inferior vena cava duplication and deep venous thrombosis | Ann Vasc Surg | 16575497 | Full Paper | |
2006 | Huang, LT; | Transcriptional analysis of ex vivo granulocyte (neutrophil) development | Thesis | Cultures were initiated with fresh or frozen G-CSF mobilized peripheral blood CD34+ cells from normal donors (AllCells, Emeryville, CA), at a seeding density of 3 x 104 cells/mL. Cells were cultured in human long-term medium (HLTM) at a pH of 7.1 an | Full Paper | |
2006 | Chen, C;Huang, X;Atakilit, A;Zhu, QS;Corey, SJ;Sheppard, D; | The Integrin alpha9beta1 contributes to granulopoiesis by enhancing granulocyte colony-stimulating factor receptor signaling | Immunity | 17137800 | Murine bone marrow or peripheral blood cells were collected at autopsy and suspended in phosphate-buffered saline (PBS). Human bone marrow cells were obtained from Allcells (Berkeley, CA). Cells were incubated with primary antibody for 30 min at 4°C, | Full Paper |
2006 | Guerzoni, C;Bardini, M;Mariani, SA;Ferrari-Amorotti, G;Neviani, P;Panno, ML;Zhang, Y;Martinez, R;Perrotti, D;Calabretta, B; | Inducible activation of CEBPB, a gene negatively regulated by BCR/ABL, inhibits proliferation and promotes differentiation of BCR/ABL-expressing cells | Blood | 16418324 | CD34+ normal bone marrow (NBM) cells from different healthy donors were purchased from AllCells (LLC, Berkley, CA) and their use was approved by the Ohio State University Institutional Review Board (IRB). Mononuclear hematopoietic cells from bone mar | Full Paper |
2006 | Notari, M;Neviani, P;Santhanam, R;Blaser, BW;Chang, JS;Galietta, A;Willis, AE;Roy, DC;Caligiuri, MA;Marcucci, G;Perrotti, D; | A MAPK/HNRPK pathway controls BCR/ABL oncogenic potential by regulating MYC mRNA translation | Blood | 16293596 | CD34+ normal bone marrow (NBM) cells from healthy donors were from AllCells (Berkeley, CA). Studies performed with human specimens from The Ohio State University Leukemia Tissue Bank (Columbus, OH), the Division of Hematology, Maisonneuve-Rosemont Ho | Full Paper |
2006 | Choi, J;Garewal, H;Riley, C;Cooke, L;Klinkhammer, T;Maddipati, S;Sud, R;Spier, C;Mahadevan, D; | Transcriptosome and Serum Cytokine Profiling of B-CLL Identifies Oncogenic Pathways in Pathogenesis and Survival. | Blood | Full Paper | ||
2006 | Greaney, P;Nahimana, A;Lagopoulos, L;Etter, AL;Aubry, D;Attinger, A;Beltraminelli, N;Huni, B;Bassi, I;Sordat, B;Demotz, S;Dupuis, M;Duchosal, MA; | A Fas agonist induces high levels of apoptosis in haematological malignancies | Leukemia research | 16181674 | CLL 1, 2 and 3, κ+). These cells were frozen in medium containing 10% DMSO within 10 h after harvesting. Haematopoietic progenitor cells from cord blood were obtained frozen from AllCells (San Mateo, CA) | Full Paper |
2006 | Chua, KN;Chai, C;Lee, PC;Tang, YN;Ramakrishna, S;Leong, KW;Mao, HQ; | Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells | Biomaterials | 16854459 | Interaction between hematopoietic stem/progenitor cells (HSPCs) and their extra cellular matrix components is an integral part of the signaling control for HSPC survival, proliferation Save | Full Paper |
2006 | La Rosée, P;Jia, T;Demehri, S;Härtel, N;de Vries, P;Bonham, L;Hollenback, D;Singer, JW;Melo, JV;Druker, BJ;Deininger, MW; | Antileukemic activity of lysophosphatidic acid acyltransferase-beta inhibitor CT32228 in chronic myelogenous leukemia sensitive and resistant to imatinib | Clinical cancer research : an official journal of the American Association for Cancer Research | 17085669 | without erythropoietin [for culture of granulocyte-macrophage colony-forming unit (CFU-GM)] were done as described previously (26). Normal bone marrow was purchased commercially (AllCells, LLC, Berkeley, CA). Patient samples were collected from patie | Full Paper |
2006 | Yang, L;Bailey, L;Baltimore, D;Wang, P; | Targeting lentiviral vectors to specific cell types in vivo | Proceedings of the National Academy of Sciences of the United States of America | 16864770 | 106) (AllCells) were incubated with concentrated virus with total TUs of 10 106 fresh human PBMCs (AllCells) were transferred by tail vein injection into each mouse. After 6 h, Save | Full Paper |
2006 | Giammona, LM;Fuhrken, PG;Papoutsakis, ET;Miller, WM; | Nicotinamide (vitamin B3) increases the polyploidisation and proplatelet formation of cultured primary human megakaryocytes | British journal of haematology | 17054670 | Unless otherwise noted, all reagents were obtained from Sigma-Aldrich (St Louis, MO, USA). Cultures were initiated with previously frozen human mobilised peripheral blood (mPB) CD34+ cells (AllCells, Berkeley, CA, USA). Cells were cultured in T-flask | Full Paper |
2006 | Wang, HJ;Yu, CL;Kishi, H;Motoki, K;Mao, ZB;Muraguchi, A; | Suppression of experimental osteoarthritis by adenovirus-mediated double gene transfer | Chinese medical journal | 16934183 | Allcells were grown to approxim ately 60% 一 70% confluence at the third passage before adenoviraI vector transduction experim ents started.The cells were washed with sterile saline Save | Full Paper |
2006 | Cabana, R;Frolova, EG;Kapoor, V;Thomas, RA;Krishan, A;Telford, WG; | The minimal instrumentation requirements for Hoechst side population analysis: stem cell analysis on low-cost flow cytometry platforms | Stem cells (Dayton, Ohio) | 16888279 | Institute Laboratory Animal Facility. Mice were euthanized by gradual CO2 exposure according to NIH guidelines. Bone marrow was extracted from femurs and tibias and used with no further fractionation. Frozen human bone marrow and cord blood were obta | Full Paper |
2006 | Yu, X;Alder, JK;Chun, JH;Friedman, AD;Heimfeld, S;Cheng, L;Civin, CI; | HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding | Stem cells (Dayton, Ohio) | 16513761 | Normal human placental/umbilical cord blood (CB) CD34+ cells were purchased from AllCells (Berkeley, CA, http://www.allcells.com). Normal BM and granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood stem cell (PBSC) CD34+ cells wer | Full Paper |
2006 | McIntosh, K;Zvonic, S;Garrett, S;Mitchell, JB;Floyd, ZE;Hammill, L;Kloster, A;Di Halvorsen, Y;Ting, JP;Storms, RW;Goh, B;Kilroy, G;Wu, X;Gimble, JM; | The immunogenicity of human adipose-derived cells: temporal changes in vitro | Stem cells (Dayton, Ohio) | 16410391 | Human bone marrow was purchased from Cambrex (Walkersville, MD, http://www.cambrex.com) or AllCells, LLC (Berkeley, CA). Bone marrow aspirates were collected with heparin and fractionated over a 1.073 g/ml density gradient (Lymphocyte Separation Medi | Full Paper |
2006 | Miura, M;Miura, Y;Padilla-Nash, HM;Molinolo, AA;Fu, B;Patel, V;Seo, BM;Sonoyama, W;Zheng, JJ;Baker, CC;Chen, W;Ried, T;Shi, S; | Accumulated chromosomal instability in murine bone marrow mesenchymal stem cells leads to malignant transformation | Stem cells (Dayton, Ohio) | 16282438 | Human bone marrow aspirates from healthy adult volunteers were purchased from AllCells, LLC (Berkeley, CA, http://www.allcells.com). To identify putative BMMSCs, single-cell suspension of 1 × 106 of bone marrow mononuclear cells was seeded into 15-cm | Full Paper |
2006 | Muramoto, GG;Chen, B;Cui, X;Chao, NJ;Chute, JP; | Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injury | Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation | 16635788 | Cryopreserved human BM CD34+ (Cambrex, Gaithersburg, MD) or CB CD34+ cells (AllCells, Berkeley, CA) were thawed, washed once, and resuspended at 1 × 106/mL in Iscove modified Dulbecco medium (IMDM; Invitrogen) containing 10% FBS and 1% penicillin/str | Full Paper |
2006 | Wang, J;Wu, X;Simonavicius, N;Tian, H;Ling, L; | Medium-chain fatty acids as ligands for orphan G protein-coupled receptor GPR84 | The Journal of biological chemistry | 16966319 | Quantitative Reverse Transcriptase-mediated PCR (Q-RT-PCR) Analysis—Total RNA from human or mouse tissues (Clontech) and human immune cells (AllCells, LLC) were treated with DNase I (Ambion) before reverse transcription. Q-RT-PCR was performed on an | Full Paper |
2006 | Wang, J;Simonavicius, N;Wu, X;Swaminath, G;Reagan, J;Tian, H;Ling, L; | Kynurenic acid as a ligand for orphan G protein-coupled receptor GPR35 | The Journal of biological chemistry | 16754668 | Cytokine Secretion—Human peripheral blood mononuclear cells and CD14 + monocytes were purchased from Allcells. Peripheral blood mononuclear cells were seeded at a density of Save | Full Paper |
2006 | Slukvin, II;Vodyanik, MA;Thomson, JA;Gumenyuk, ME;Choi, KD; | Directed differentiation of human embryonic stem cells into functional dendritic cells through the myeloid pathway | Journal of immunology (Baltimore, Md. : 1950) | 16493050 | Adult PBMC were purchased from AllCells. Monocytes were isolated by adherence to plastic and were cultured for 6 days in RPMI 1640 (Invitrogen Life Technologies) containing 1% FBS (HyClone) in the presence of 100 ng/ml GM-CSF and 100 ng/ml IL-4 with | Full Paper |
2006 | Zhang, W;Hirschler-Laszkiewicz, I;Tong, Q;Conrad, K;Sun, SC;Penn, L;Barber, DL;Stahl, R;Carey, DJ;Cheung, JY;Miller, BA; | TRPM2 is an ion channel that modulates hematopoietic cell death through activation of caspases and PARP cleavage | American journal of physiology. Cell physiology | 16306129 | RNA from CD34 + cells and fresh monocytes was purchased from AllCells (Foster City, CA). cDNA was prepared from RNA with the Superscript first-strand synthesis system (Invitrogen, Save | Full Paper |
2006 | Geddis, AE;Kaushansky, K; | Endomitotic megakaryocytes form a midzone in anaphase but have a deficiency in cleavage furrow formation | Cell cycle (Georgetown, Tex.) | 16552179 | albumin gradient. For human MKs, CD34+ cells were purchased from AllCells (Berkley, CA) and thawed according to manufacturer’s instructions. The cells were then differentiated by culture in StemPro (Invitrogen) containing 100 ng/ml human TPO, 10 ng/m | Full Paper |
2006 | Shen, Y;Chow, J;Wang, Z;Fan, G; | Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cells | Human molecular genetics | 16870691 | Medium was changed everyday for optimal cell growth. Fetal astrocytes were purchased from AllCells LLC., and cultures were expanded as suggested by the vendor. Fetal brain tissue was obtained from the Surgical Pathology Laboratory at UCLA (Dr. Michae | Full Paper |
2006 | Polson, AG;Zheng, B;Elkins, K;Chang, W;Du, C;Dowd, P;Yen, L;Tan, C;Hongo, JA;Koeppen, H;Ebens, A; | Expression pattern of the human FcRH/IRTA receptors in normal tissue and in B-chronic lymphocytic leukemia | International immunology | 16849395 | Human blood samples were collected from healthy individuals through the Genentech research blood program; bone marrow was obtained from AllCells (Berkeley, CA, USA) and Save | Full Paper |
2006 | Zhang, X;Kim, J;Ruthazer, R;McDevitt, MA;Wazer, DE;Paulson, KE;Yee, AS; | The HBP1 transcriptional repressor participates in RAS-induced premature senescence | Molecular and cellular biology | 16966377 | Oncogene-mediated premature senescence has emerged as a potential tumor-suppressive mechanism in early cancer transitions. Previous work shows that RAS and p38 MAPK Save | Full Paper |
2006 | Feng, Q;Chai, C;Jiang, XS;Leong, KW;Mao, HQ; | Expansion of engrafting human hematopoietic stem/progenitor cells in three-dimensional scaffolds with surface-immobilized fibronectin | Journal of biomedical materials research. Part A | 16739181 | Frozen human CB CD34+ cells were purchased from All-Cells (San Mateo, CA), which were obtained from normal volunteers participating in an Institute Reviewing Board (IRB) approved donor program (AllCells). Cells (92% CD34+, 95% viable) were thawed acc | Full Paper |
2006 | Weigel-Kelley, KA;Yoder, MC;Chen, L;Srivastava, A; | Role of integrin cross-regulation in parvovirus B19 targeting | Human gene therapy | 16972759 | Primary human CD71 erythroid progenitor cells were purchased from AllCells (Berkeley, CA) and received in Iscove's modified Dulbecco's medium (IMDM)- 0.5% bovine serum Save | Full Paper |
2006 | Braselmann, S;Taylor, V;Zhao, H;Wang, S;Sylvain, C;Baluom, M;Qu, K;Herlaar, E;Lau, A;Young, C;Wong, BR;Lovell, S;Sun, T;Park, G;Argade, A;Jurcevic, S;Pine, P;Singh, R;Grossbard, EB;Payan, DG;Masuda, ES; | R406, an orally available spleen tyrosine kinase inhibitor blocks fc receptor signaling and reduces immune complex-mediated inflammation | The Journal of pharmacology and experimental therapeutics | 16946104 | Stimulation of Macrophages Derived from Primary Monocytes with IgG or LPS. Human primary macrophages were derived from CD14+ peripheral blood mononuclear cell (AllCells, LLC, Emeryville, CA) according to the protocol specified in the monocyte isolati | Full Paper |
2006 | Xu, H;Othman, SF;Hong, L;Peptan, IA;Magin, RL; | Magnetic resonance microscopy for monitoring osteogenesis in tissue-engineered construct in vitro | Physics in medicine and biology | 16424591 | Mesenchymal stem cells (MSCs) isolated from fresh adult human bone marrow were provided commercially by AllCells (AllCells LLC, Berkeley, CA). Nucleated cells were incubated in Save | Full Paper |
2006 | Troken, A;Marion, N;Mao, J; | HETEROTYPIC ADIPOGENIC PHENOTYPE WHEN EMPLOYING CHONDROGENIC TREATMENT OF HUMAN MESENCHYMAL STEM CELLS | ASAIO Journal | Donated hMSCs (AllCells, LLC) were isolated and expanded using basal media. Upon 90% confluence, the cultures were plated at a density of 24,000 cells/cm 2 in 6-well plates. Three Save | Full Paper | |
2006 | Yamada, H;Kunisato, A;Kawahara, M;Tahimic, CGT;Ren, X;Ueda, H;Nagamune, T;Katoh, M;Inoue, T;Nishikawa, M;Oshimura, M; | Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome | Journal of human genetics | 16333524 | Both EGFP-21ΔqHAC vectors and ScFvg-21ΔqHAC vectors retained in CHO cells were transferred to 1×107 hBM MNCs (AllCells, LLC, Berkeley, CA, USA) or 1×106 hCB CD34+ cells by microcell-mediated chromosome transfer (MMCT) as previously described (Doherty | Full Paper |
2006 | Wang, T;Blatt, LM;Seiwert, SD; | Immunomodulatory activities of IFN-gamma1b in combination with type I IFN: implications for the use of IFN-gamma1b in the treatment of chronic HCV infections | Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research | 16800786 | IL-4 for 2 weeks), and immature pDCs (IFN-producing cells [IPC], defined as CD11C, CD123, and Lin) were purchased from AllCells, LLC (Berkeley, CA). The procedures for their collection were approved by an Institute Review Board and complied with loca | Full Paper |
2006 | Duramad, P;Tager, IB;Leikauf, J;Eskenazi, B;Holland, NT; | Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1 | Journal of applied toxicology : JAT | 16871525 | emale) who were sampled repeatedly, for experiments1-13, or were purchased (n=8) from AllCells (Berkeley,CA) for experiments 14 -19. Specimens were collecteddirectly into vacutainers with heparin and processedwithin 2 | Full Paper |
2006 | Aslan, H;Zilberman, Y;Arbeli, V;Sheyn, D;Matan, Y;Liebergall, M;Li, JZ;Helm, GA;Gazit, D;Gazit, Z; | Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regeneration | Tissue engineering | 16674300 | Human mesenchymal stem cells (hMSCs) were isolated from the bone marrow of an anonymous healthy donor (AllCells, Berkeley, CA), cultureexpanded and seeded in 6-well plates at a density of 30,000 cells/well. For | Full Paper |
2006 | Peptan, IA;Hong, L;Xu, H;Magin, RL; | MR assessment of osteogenic differentiation in tissue-engineered constructs | Tissue engineering | 16674297 | Human bone marrow cells provided commercially(AllCells Limited Liability Company, Berkeley, CA)were plated at a density of 107nucleated cells per 100mm petri dish and incubated in a culture medium com-posed of Dulbecco’s modified | Full Paper |
2006 | Stroke, IL;Cole, AG;Simhadri, S;Brescia, MR;Desai, M;Zhang, JJ;Merritt, JR;Appell, KC;Henderson, I;Webb, ML; | Identification of CXCR3 receptor agonists in combinatorial small-molecule libraries | Biochemical and biophysical research communications | 16930533 | Human peripheral blood mononuclear cells (AllCells, Berkeley, CA) were cultured for 3 days with 10 μg/mL phytohemagglutinin, then for 4-7 days with 100 U/mL interleukin-2 ( Save | Full Paper |
2006 | Sugo, T;Tachimoto, H;Chikatsu, T;Murakami, Y;Kikukawa, Y;Sato, S;Kikuchi, K;Nagi, T;Harada, M;Ogi, K;Ebisawa, M;Mori, M; | Identification of a lysophosphatidylserine receptor on mast cells | Biochemical and biophysical research communications | 16460680 | The total RNAs from human basophils and eosinophils were purchased from AllCells Limited Liability Company (Berkeley, CA). Total RNA was reverse transcribed using Superscript Save | Full Paper |
2006 | Ong, HT;Timm, MM;Greipp, PR;Witzig, TE;Dispenzieri, A;Russell, SJ;Peng, KW; | Oncolytic measles virus targets high CD46 expression on multiple myeloma cells | Experimental hematology | 16728275 | Fresh bone marrow aspirate from a healthy donor was purchased from AllCells (Berkeley, CA, USA). The mononuclear cell fraction was isolated by Ficoll-Hypaque gradient and used in Save | Full Paper |
2006 | Yoshikubo, T;Inoue, T;Noguchi, M;Okabe, H; | Differentiation and maintenance of mast cells from CD34(+) human cord blood cells | Experimental hematology | 16543066 | Frozen human umbilical cord blood CD34+ cells were purchased from AllCells, LLC (Berkeley, CA, USA). The mouse bone marrow-derived stroma cell line MS-5 was kindly provided by K. Itoh [23]. MS-5 cells were maintained in a minimal essential medium α ( | Full Paper |
2006 | Hong, L;Peptan, IA;Xu, H;Magin, RL; | Nondestructive evaluation of osteogenic differentiation in tissue-engineered constructs | Journal of orthopaedic research : official publication of the Orthopaedic Research Society | 16583444 | Healthy fresh human bone marrow was provided commercially (AllCells Limited Liability Company, Emeryville, CA) and plated at a density of 107 nucleated cells per 100 mm petri dish. The cells were incubated in a basic medium of Dulbecco’s Modified Eag | Full Paper |
2006 | Cole, AG;Stroke, IL;Brescia, MR;Simhadri, S;Zhang, JJ;Hussain, Z;Snider, M;Haskell, C;Ribeiro, S;Appell, KC;Henderson, I;Webb, ML; | Identification and initial evaluation of 4-N-aryl-[1,4]diazepane ureas as potent CXCR3 antagonists | Bioorganic & medicinal chemistry letters | 16213722 | 一般社団法人情 処理学会では複写複製および転載複製に係る著作権を学術著作権協会に委託 しています. 当該利用をご希望の方は, 学術著作権協会 が提供している複製利用許諾システ Save | Full Paper |
2006 | Liu, WM;Li, R;Sun, JZ;Wang, J;Tsai, J;Wen, W;Kohlmann, A;Williams, PM; | PQN and DQN: algorithms for expression microarrays | Journal of theoretical biology | 16889801 | Here, we disclose two triplets of experiments using 3 ml fresh bone marrow from a healthy donor obtained from AllCells, LLC (Berkeley, CA). The sample was stored at for 48 h. Then total RNA was purified from the sample with RNeasy mini kit (Qiagen, | Full Paper |
2006 | Doshi, M;Koyanagi, M;Nakahara, M;Saeki, K;Saeki, K;Yuo, A; | Identification of human neutrophils during experimentally induced inflammation in mice with transplanted CD34+ cells from human umbilical cord blood | International journal of hematology | 17050197 | MBR1520-3 x-ray source (Hitachi Medical, Tokyo, Japan). After 24 hours, mice received intravenous transplants of 1.8 × 105 human CB CD34+ cells (AllCells, Berkeley, CA, USA) or vehicle (saline). The purity and viability of the CB CD34+ cells were gre | Full Paper |
2006 | Marion, N;Mao, J; | Mesenchymal Stem Cells and Tissue Engineering | Methods in Enzymology | Human Bone Marrow-Derived MSCs • Ficoll‐Paque—room temperature (e.g., StemCells, Inc., Vancouver, BC, Canada). • Bone marrow sample—room temperature (e.g., AllCells, LLC, Berkeley, CA). • 10 ml marrow + 5 ml DPBS + 125 U/ml heparin (total volume, 1 | Full Paper | |
2006 | Hawley, R;Ramezani, A;Hawley, T; | Hematopoietic Stem Cells | Adult Stem Cells | peripheral blood cells after informed consent in conformity with a human subjects protocol approved by an institutional review board, or purchase from a commercial source [eg, AllCells Save | Full Paper | |
2006 | Nudera, J; | Mechanical stresses elicit osteogenic changes in HMSCS encapsulated in a polyethylene glycol scaffold | Thesis | The samples of fresh bone marrow (Allcells, Berkeley, CA) were delivered in 10ml amoimts. Both of the patients tested negative for human immunodeficiency virus (HIV), hepatitis B Save | Full Paper | |
2006 | Crot, CA; | Probing the solid-liquid interface of synthetic biomaterials via x-ray scattering techniques | Thesis | The general biocompatibility of the allylamine films is compared with that of the native oxide surface of Ti by culturing human mesenchymal stems cells (hMSCs) isolated from healthy donors (AllCells, Berkeley, CA) (82). HMSCs are plated in monolayer | Full Paper | |
2006 | Lee, CH;Moioli, EK;Mao, JJ; | Fibroblastic differentiation of human mesenchymal stem cells using connective tissue growth factor | Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference | 17946857 | Human bone marrow samples taken from healthy donors ranging from 18 to 45 years of age were purchased from AllCells (Berkeley, CA). The bone marrows were prepared to isolate hMSCs per our previous methods [10], [11]. Briefly, the whole bone marrow sa | Full Paper |
2006 | Moinnes, JJ;Vidula, N;Halim, N;Othman, SF; | Ultrasound accelerated bone tissue engineering monitored with magnetic resonance microscopy | Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference | 17946836 | Mesenchymal stem cells (MSCs) isolated from fresh adult human bone marrow were provided by AllCells (AllCells LLC, Berkeley, CA). Nucleated cells were expanded via incubation Save | Full Paper |
2006 | Dravid, G;Hammond, H;Cheng, L; | Culture of human embryonic stem cells on human and mouse feeder cells | Methods in molecular biology (Clifton, N.J.) | 16881511 | mitomycin C-treated (Speciality Media; cat. no. PMEF-CF [mitomycin-treated]) (see Note 5 ). 3. Normal breast feeder cells: CCD-1087sk (American Type Culture Collection, Manassas, VA; cat. no. CRL-2104). 4. Bone marrow mononuclear cells (AllCells; L | Full Paper |
2006 | MacDonald, M; | The DataGridView | Pro .NET 2.0 Windows Forms and Custom Controls in C# | Similar to AllCells, except the DataGridView only considers the rows that are currently visible at the time the property is set. This option is used to improve performance for large grids. Save | Full Paper | |
2006 | Crossman, LC; | Predictors of imatinib response in patients with chronic myeloid leukaemia | Thesis | allowing us to compare the groups pre-imatinib. All patients consented to enter this study (Appendix 2). Four samples of healthy BM MNC's were obtained from AllCells (Berkeley, CA), to act as normal controls | Full Paper | |
2006 | Chen, C; | Megakaryocytic* transcriptional program and developmental plasticity | Thesis | Mk and G cell cultures were initiated with fresh G-CSF-mobilized human peripheral blood CD34+ cells from normal donors (AllCells; Berkeley, CA). For Mk cultures, cells were cultured in X-VIVO 20 serum-free medium (BioWhittaker; Walkersville, MD) sup | Full Paper | |
2006 | Yourek, G; | Effects of differentiation and shears stress on stem cell gene expression and nanomechanics | Thesis | Whole bone marrow (AllCells, Berkeley, CA) was mixed with "RosetteSep®" (StemCell Technologies; Vancouver, BC) (50 pL per 1 mL of bone marrow) and incubated for 20 minutes at room temperature (RT). The "RosetteSep®" antibody cocktail crosslinks unwan | Full Paper | |
2006 | Clark, PA; | Modulating bone ingrowth into titanium implants by growth factor delivery and mechanical loading | Thesis | Human mesenchymal stem cell isolation and expansion Human mesenchymal stem cells (hMSCs) were isolated from bone marrow. Whole human bone marrow was purchased commercially (AllCells LLC, Berkeley, CA). Negative selection (RosetteSep, StemCell | Full Paper | |
2006 | Charoonpatrapong, K; | The roles of nuclear matrix protein 4 and high mobility group box 1 in mediating the osteoblast response to anabolic agents | Thesis | Human cord blood-derived osteoclastogenesis was performed as described previously (Fuson & Galvin, 2002) with slight modification. Frozen human cord blood mononuclear cells (CBMNCs) were purchased from AllCells (Berkeley, CA). CBMNCs were quickly tha | Full Paper | |
2005 | De Vry, CG;Valdez, M;Lazarov, M;Muhr, E;Buelow, R;Fong, T;Iyer, S; | Topical application of a novel immunomodulatory peptide, RDP58, reduces skin inflammation in the phorbol ester-induced dermatitis model | J. Invest. Dermatol. | 16117788 | 10% neutral-buffered formalin (Fisher Scientific). The preparation of tissues by embedding in paraffin, sectioning, and staining was carried out by QualTek Molecular Laboratories (Santa Barbara, California). A series of ear cross | Full Paper |
2005 | Finnberg, N;Gruber, JJ;Fei, P;Rudolph, D;Bric, A;Kim, SH;Burns, TF;Ajuha, H;Page, R;Wu, GS;Chen, Y;McKenna, WG;Bernhard, E;Lowe, S;Mak, T;El-Deiry, WS; | DR5 knockout mice are compromised in radiation-induced apoptosis | Mol. Cell. Biol. | 15713653 | ... ts separated on a 2% agarose gel. Mean band density was determined with NIH Image 1.62 and normalized to the band density of 18S rRNA. IN SITU HYBRIDIZATION. In situ hybridization was performed by Phylogeny Inc. as described (17). Briefly, fet | Full Paper |
2005 | Schirmer, A;Gadkari, R;Reeves, CD;Ibrahim, F;DeLong, EF;Hutchinson, CR; | Metagenomic analysis reveals diverse polyketide synthase gene clusters in microorganisms associated with the marine sponge Discodermia dissoluta | Appl. Environ. Microbiol. | 16085882 | We thank Dan Morse (University of California, Santa Barbara) and David Myles for obtaining Discodermia dissoluta, Steve Bernstein (QualTek Molecular Laboratories, Santa Barbara, CA) for help with microscopy on sponge thin sections, and Ralph Reid for | Full Paper |
2005 | Pyle-Chenault, RA;Stolk, JA;Molesh, DA;Boyle-Harlan, D;McNeill, PD;Repasky, EA;Jiang, Z;Fanger, GR;Xu, J; | VSGP/F-spondin: a new ovarian cancer marker | Tumour Biol. | 16103746 | Rockford, Ill., USA). Immunohistochemistry was performed with affinity-purified rabbit polyclonal antibody on formalin-fixed, paraffin-embedded tissues by QualTek Molecular Laboratories (Santa Barbara, Calif., USA). Steam heat | Full Paper |
2005 | Wang, B;Berger, M;Masters, G;Albone, E;Yang, Q;Sheedy, J;Kirksey, Y;Grimm, L;Wang, B;Singleton, J;Soltis, D; | Radiotherapy of human xenograft NSCLC tumors in nude mice with a 90Y-labeled anti-tissue factor antibody | Cancer Biother. Radiopharm. | 15989475 | An IHC study of TF expression in human tissues was performed at QualTek Labs (Newton, PA). Briefly, formalin-fixed, paraffin-embedded sec- tions from human NSCLC tumors and different normal human tissues were taken from QualTek's human tissue bank | Full Paper |
2005 | Urnov, FD;Miller, JC;Lee, YL;Beausejour, CM;Rock, JM;Augustus, S;Jamieson, AC;Porteus, MH;Gregory, PD;Holmes, MC; | Highly efficient endogenous human gene correction using designed zinc-finger nucleases | Nature | 15806097 | Human CD4+ T cells (AllCells) were grown according to the supplier's instructions. Cells were activated using 20 Units per ml IL-2 (R&D Systems) and αCD3/CD28 beads (Miltenyi Biotec, T Cell Activation/Expansion kit), and transfected with ZFN and dono | Full Paper |
2005 | Freud, AG;Becknell, B;Roychowdhury, S;Mao, HC;Ferketich, AK;Nuovo, GJ;Hughes, TL;Marburger, TB;Sung, J;Baiocchi, RA;Guimond, M;Caligiuri, MA; | A human CD34(+) subset resides in lymph nodes and differentiates into CD56bright natural killer cells | Immunity | 15780987 | Fresh human BM was donated or purchased from AllCells, LLC (Berkeley, CA) and was received within 24 hr of harvest. PB leukopaks were obtained from the American Red Cross. BM Save | Full Paper |
2005 | Neviani, P;Santhanam, R;Trotta, R;Notari, M;Blaser, BW;Liu, S;Mao, H;Chang, JS;Galietta, A;Uttam, A;Roy, DC;Valtieri, M;Bruner-Klisovic, R;Caligiuri, MA;Bloomfield, CD;Marcucci, G;Perrotti, D; | The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABL-regulated SET protein | Cancer cell | 16286244 | The oncogenic BCR/ABL kinase activity induces and maintains chronic myelogenous leukemia (CML). We show here that, in BCR/ABL-transformed cells and CML blast crisis (CML-BC) Save | Full Paper |
2005 | Crossman, LC;Druker, BJ;Deininger, MW;Pirmohamed, M;Wang, L;Clark, RE; | hOCT 1 and resistance to imatinib | Blood | 16033955 | mononuclear cells (MNCs) cryopreserved immediately prior to imatinib therapy, and all NRs had BM MNCs stored after 9 to 15 months of imatinib treatment. Four normal BM MNC samples were obtained from AllCells (Berkeley, CA) | Full Paper |
2005 | Corbin, AS;Demehri, S;Griswold, IJ;Wang, Y;Metcalf, CA;Sundaramoorthi, R;Shakespeare, WC;Snodgrass, J;Wardwell, S;Dalgarno, D;Iuliucci, J;Sawyer, TK;Heinrich, MC;Druker, BJ;Deininger, MW; | In vitro and in vivo activity of ATP-based kinase inhibitors AP23464 and AP23848 against activation-loop mutants of Kit | Blood | 15746079 | Mononuclear cells from 4 independent commercially available normal bone marrow samples (AllCells, Berkeley, CA) were cultured in duplicate plates with doses of AP23464 ranging Save;Hematopoietic colony forming assays: Mononuclear cells from four in | Full Paper |
2005 | Orita, T;Tsunoda, H;Yabuta, N;Nakano, K;Yoshino, T;Hirata, Y;Ohtomo, T;Nezu, J;Sakumoto, H;Ono, K;Saito, M;Kumagai, E;Nanami, M;Kaneko, A;Yoshikubo, T;Tsuchiya, M; | A novel therapeutic approach for thrombocytopenia by minibody agonist of the thrombopoietin receptor | Blood | 15374889 | CD34-positive cells (AllCells, LLC, Berkeley, CA) derived from human bone marrow were cultured with rhTPO or VB22B sc(Fv)2 in the presence of 0.2 ng/mL human IL-3 (PeproTech, London, UK) for 11 days at 37°C in Iscove's Modified Dulbecco's Medium (MDM | Full Paper |
2005 | Choi, J;Simons, B;Riley, C;Klinkhammer, T;Cooke, L;Spier, C;Sud, R;Hehn, S;Garewal, H;Mahadevan, D; | Transcriptosome Profiling of B-CLL Identifies WNT-3A and ROR-1 as an Autocrine Mechanism in Cell Survival. | Blood | Full Paper | ||
2005 | Clark, RA;Yamanaka, K;Bai, M;Dowgiert, R;Kupper, TS; | Human skin cells support thymus-independent T cell development | The Journal of clinical investigation | 16224538 | For IFN-γ treated matrices, 50 ng/ml IFN-γ (R&D Systems) was included in the culture medium from plating until day 6. On day 7, 10,000 normal human bone marrow AC133+ precursor cells (AllCells) were added to each matrix, and the unit was cultured in | Full Paper |
2005 | Pan, F;Means, AR;Liu, JO; | Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation | The EMBO journal | 15902271 | Human peripheral blood mononuclear cells (PMBCs) were obtained from AllCells (Berkeley, CA). Naïve CD4+ T cells were purified by depletion of memory T cells as well as non-CD4+ cells, followed by positive selection with magnetic activated cell separa | Full Paper |
2005 | O'Hare, T;Walters, DK;Stoffregen, EP;Sherbenou, DW;Heinrich, MC;Deininger, MW;Druker, BJ; | Combined Abl inhibitor therapy for minimizing drug resistance in chronic myeloid leukemia: Src/Abl inhibitors are compatible with imatinib | Clinical cancer research : an official journal of the American Association for Cancer Research | 16203792 | Samples were obtained with informed consent from four CML patients or from a tissue repository (AllCells, Berkeley, CA). The CML samples were screened for kinase domain mutations Save | Full Paper |
2005 | Kikuchi, J;Ozaki, H;Nonomura, C;Shinohara, H;Iguchi, S;Nojiri, H;Hamada, H;Kiuchi, A;Nakamura, M; | Transfection of antisense core 2 beta1,6-N-acetylglucosaminyltransferase-1 cDNA suppresses selectin ligand expression and tissue infiltration of B-cell precursor leukemia cells | Leukemia | 16179912 | (CB) was drawn from umbilical cords of cesarean section and normal full-term deliveries. Mononuclear cells (MNCs) were enriched by Ficoll density gradients and subjected to analysis. Bone marrow (BM) derived MNCs were purchased from AllCells (Berkele | Full Paper |
2005 | D'Costa, J;Chaudhuri, S;Civin, CI;Friedman, AD; | CBFbeta-SMMHC slows proliferation of primary murine and human myeloid progenitors | Leukemia | 15815715 | Human cord blood CD34+ cells (AllCells Inc., San Mateo, CA, USA) or cryopreserved human mobilized peripheral blood CD34+ cells from the Johns Hopkins cell bank (from donors who gave informed consent on an IRB-approved protocol) were placed directly i | Full Paper |
2005 | Leugers, S;Allison, D;Pronold, B;Rennebeck, G;Van Zant, G;Tario, J;Swartzwelder, F;Donahue, L; | Stemline Hematopoietic Stem Cell Expansion Medium, a Serum-Free Medium for the Expansion of CD34+ Hematopoietic Stem Cells and Progenitors | Animal Cell Technology Meets Genomics | Cell Preparation. For all experiments, cryopreserved, human CD34+ cells were obtained from independent suppliers (Stemgenix; AllCells, LLC) and were handled in a manner consistent with the supplier’s instructions with regard to storage and reconstitu | Full Paper | |
2005 | Wang, R;Arevalo-Herrera, M;Gardner, MJ;Bonelo, A;Carlton, JM;Gomez, A;Vera, O;Soto, L;Vergara, J;Bidwell, SL;Domingo, A;Fraser, CM;Herrera, S; | Immune responses to Plasmodium vivax pre-erythrocytic stage antigens in naturally exposed Duffy-negative humans: a potential model for identification of liver-stage antigens | European journal of immunology | 15864779 | vivax blood-stage parasites, 2 were living in California and their PBMC were purchased from AllCells (AllCells, LLC, Berkeley, CA) and used in this study as controls. All volunteers Save | Full Paper |
2005 | Mahadevan, D;Spier, C;Della Croce, K;Miller, S;George, B;Riley, C;Warner, S;Grogan, TM;Miller, TP; | Transcript profiling in peripheral T-cell lymphoma, not otherwise specified, and diffuse large B-cell lymphoma identifies distinct tumor profile signatures | Molecular cancer therapeutics | 16373702 | Control RNA was obtained from normal peripheral B cells (AllCells, Berkeley, CA), normal peripheral T cells (AllCells), and a snap-frozen normal lymph node (Department of Pathology, Save | Full Paper |
2005 | Siatskas, C;Underwood, J;Ramezani, A;Hawley, RG;Medin, JA; | Specific pharmacological dimerization of KDR in lentivirally transduced human hematopoietic cells activates anti-apoptotic and proliferative mechanisms | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 16076962 | CD34 + -selected cord blood cells were purchased from AllCells (Berkeley, CA). Cells were maintained at 37C in a humidified atmosphere of 5% CO 2 . Addition of rh stem cell factor ( Save | Full Paper |
2005 | Rao, PE;Petrone, AL;Ponath, PD; | Differentiation and expansion of T cells with regulatory function from human peripheral lymphocytes by stimulation in the presence of TGF-{beta} | Journal of immunology (Baltimore, Md. : 1950) | 15661903 | For T cell differentiation using human cord blood naive CD4 + T cells, frozen CD4 + CD45RA + cells isolated using magnetic beads were obtained from Allcells. Cells were thawed and Save | Full Paper |
2005 | Casida, JE;Quistad, GB; | Serine hydrolase targets of organophosphorus toxicants | Chemico-biological interactions | 16243304 | Whole human blood from seven subjects and lymphocytes (isolated by aphaeresis using a leukopac filter) from three subjects were obtained from AllCells LLC (Emeryville, CA). Save | Full Paper |
2005 | Patel, S;Mehta-Damani, A;Shu, H;Le Pecq, JB; | An analysis of variability in the manufacturing of dexosomes: implications for development of an autologous therapy | Biotechnology and bioengineering | 16037986 | Peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers consisting of 111 leukopacks were obtained from AllCells Inc. (Berkeley, CA), under a protocol approved Save | Full Paper |
2005 | Hong, L;Peptan, I;Clark, P;Mao, JJ; | Ex vivo adipose tissue engineering by human marrow stromal cell seeded gelatin sponge | Annals of biomedical engineering | 15909657 | Human bone MSCs were isolated from fresh bone marrow of adult males and provided commercially by AllCells (AllCells Limited Liability Company, CA.). Nucleated cells of the fresh bone marrow were counted under inverted microscope, plated at a density | Full Paper |
2005 | Murmann, AE;Gao, J;Encinosa, M;Gautier, M;Peter, ME;Eils, R;Lichter, P;Rowley, JD; | Local gene density predicts the spatial position of genetic loci in the interphase nucleus | Experimental cell research | 16202404 | Primary human bone marrow cells (CD34+ and CD33+) were purchased from AllCells Inc. (Berkeley, CA). Chinese muntjac fibroblasts, isolated from tissue of a stillbirth Chinese muntjac fawn provided by the University of Bielefeld, Department of Animal B | Full Paper |
2005 | Alhadlaq, A;Tang, M;Mao, JJ; | Engineered adipose tissue from human mesenchymal stem cells maintains predefined shape and dimension: implications in soft tissue augmentation and reconstruction | Tissue engineering | 15869434 | Human MSCs (hMSCs) were isolated from whole bone marrow aspirates of the iliac crest of a healthy 22- year-old male donor (AllCells, Berkeley, CA). Briefly, cells were enriched by adding RosetteSep mesenchymal enrichment cocktail (StemCell Technologi | Full Paper |
2005 | Marion, N;Liang, W;Liang, W;Reilly, G;Day, D;Rahaman, M;Mao, J; | Borate Glass Supports the In Vitro Osteogenic Differentiation of Human Mesenchymal Stem Cells | Mechanics of Advanced Materials and Structures | Bone marrow samples taken from healthy donors ranging from 18 to 45 years of age were purchased from AllCells (Berkeley, CA). Human bone marrow derived mesenchymal stem Save | Full Paper | |
2005 | Lesniak, MS;Kelleher, E;Pardoll, D;Cui, Y; | Targeted gene therapy to antigen-presenting cells in the central nervous system using hematopoietic stem cells | Neurological research | 16354542 | Human G-CSF-mobilized peripheral blood or cord blood CD34z cells were obtained from AllCells (San Mateo, CA). CD34z cells were purified by immunomagnetic selection (Miltenyi Save | Full Paper |
2005 | Ivanov, M;Kraiko, A; | Numerical solution of the direct-problem of mixed nozzle flow | Fluid Dynamics | Finally, P_I/i, k-1/2 and P-1/2, k-1/2 are determined from the resulting u and v and the given (constant for allcells) entropy and stagnationenthalpy. The calculations showed that the Save | Full Paper | |
2005 | Leung, G;Tang, H;McGuinness, R;Verdonk, E;Michelotti, J;Liu, V; | Cellular Dielectric Spectroscopy: A Label-Free Technology for Drug Discovery | JALA: Journal of the Association for Laboratory Automation | HeLa (CCL-2), and U937 (CRL-1593.2)53 were obtained from ATCC (Manassas, VA). Primary neutrophils were purchased from AllCells, LLC (Berkeley, CA). HeLa cells were grown in minimal essential medium (MEM); U937 cells were grown in RPMI-1640; CHOm1 cel | Full Paper | |
2005 | Owens, BM;Hawley, RG;Spain, LM; | Retroviral transduction in fetal thymic organ culture | Methods in molecular medicine | 15492404 | samples are also available from several commercial sources, for example, AllCells, (Berkeley, CA) or Cambrex BioScience Walkersville, (Walkersville, MD). The thymocyte progenitor population can be transduced with retroviral constructs using VSV-G-pse | Full Paper |
2005 | Varma, C; | Quantitative isoforrm profiling & isoform convergence | Thesis | Samples of human peripheral blood purified B cells, human cord blood purified B cells, and human adult whole bone marrow cells derived from normal (i.e. nondiseased) individuals were purchased from ALLCELLS, LLC, Berkeley, California USA. Each sample | Full Paper | |
2005 | Gao, J; | Quantitative Analysis of Chromosome/Gene Spatial Distribution | Experimental Cell Research | CD34+ and CD33+ cells were primary hematopoietic stem cells and myeloid cells, respectively, (purchased from AllCells, LLC., CA) isolated from bone marrow with a MACS kit, purity of Save | Full Paper | |
2005 | Gilison, D; | The role of telomerase expression in normal development and cancer | Thesis | Fresh human bone marrow was obtained with informed consent from healthy volunteers (AllCells, and Department of Bone Marrow Transplantation, Stanford University School of Medicine). Fresh human cord blood was obtained with informed consent (Departmen | Full Paper | |
2005 | Windgassen, D; | Der technischen Fakultaet der Universitaet Erlangen-Nuernberg zur Erlangung des Grades | Thesis | Healthy-donor PBMCs (AllCells, Berkeley, CA) were used to select T-cell subsets (CD4(+) and CD8(+) negative selections, Miltenyi Biotech, BergischGladbach, Germany). Briefly, non-CD4(+), CD8(+), i.e. CD4(+) or CD8(+) T cells, γ/δ T cells, B cells, NK | Full Paper | |
2004 | Douglas, SA;Naselsky, D;Ao, Z;Disa, J;Herold, CL;Lynch, F;Aiyar, NV; | Identification and pharmacological characterization of native, functional human urotensin-II receptors in rhabdomyosarcoma cell lines | Br. J. Pharmacol. | 15210573 | Search for more papers by this author. Frank Lynch QualTek Molecular Laboratories Inc., Newtown, PA 18940, USA Search for more papers by this author. Frank Lynch QualTek Molecular Laboratories Inc., Newtown, PA 18940, USA ;nch Frank 2 Aiyar Na | Full Paper |
2004 | Bikle, DD;Chang, S;Crumrine, D;Elalieh, H;Man, MQ;Choi, EH;Dardenne, O;Xie, Z;Arnaud, RS;Feingold, K;Elias, PM; | 25 Hydroxyvitamin D 1 alpha-hydroxylase is required for optimal epidermal differentiation and permeability barrier homeostasis | J. Invest. Dermatol. | 15102089 | Peroxidase activity was revealed with DAB substrate (QualTek Laboratories, Santa Barbara, California) followed by counterstaining with methyl green or hematoxylin. Omitting the first antibodies resulted in no signal, indicating the specificity of imm | Full Paper |
2004 | Yu, J;He, S;Friedman, JS;Akimoto, M;Ghosh, D;Mears, AJ;Hicks, D;Swaroop, A; | Altered expression of genes of the Bmp/Smad and Wnt/calcium signaling pathways in the cone-only Nrl-/- mouse retina, revealed by gene profiling using custom cDNA microarrays | J. Biol. Chem. | 15292180 | The labeled transcripts were subjected to alkali hydrolysis (to give an average size of 70 nucleotides) and then hybridized overnight to 5- to 7-μm serial sections of adult mouse retina using standard protocols (Phylogeny Inc., Columbus, OH) | Full Paper |
2004 | Lynch, F; | The use of an interactive computerized daily schedule in a busy interventional radiology practice increases efficiency | J Am Coll Radiol | 17411739 | Full Paper | |
2004 | Kalos, M;Askaa, J;Hylander, BL;Repasky, EA;Cai, F;Vedvick, T;Reed, SG;Wright, GL;Fanger, GR; | Prostein expression is highly restricted to normal and malignant prostate tissues | Prostate | 15176054 | We thank Patrick Roche at the Mayo Clinic and Frank Lynch at Qualtek for performing immunohistochemistry experiments and Masazumi Matsamura for initial work on the generation of the 10E3-G4-D3 monoclonal antibody | Full Paper |
2004 | Tasheva, ES;Ke, A;Conrad, GW; | Analysis of the expression of chondroadherin in mouse ocular and non-ocular tissues | Mol. Vis. | 15332017 | mmol, Amersham Biosciences Corp., Piscataway, NJ). For the ISH experiments, custom service from Phylogeny Inc. (Co- lumbus, OH) was used as previously described [30]. Antibodies: A polyclonal antibody against mouse | Full Paper |
2004 | Tasheva, ES;Ke, A;Deng, Y;Jun, C;Takemoto, LJ;Koester, A;Conrad, GW; | Differentially expressed genes in the lens of mimecan-null mice | Mol. Vis. | 15215744 | secondary antibody was reacted with avidin-biotin-peroxidase complex solution (Vector Laboratories Inc., Burlingame, CA), with immunoreactivity developed in 3,3'-diaminobenzidine (DAB). In situ hybridization: For the ISH experiments, the cus- tom ser | Full Paper |
2004 | Seo, BM;Miura, M;Gronthos, S;Bartold, PM;Batouli, S;Brahim, J;Young, M;Robey, PG;Wang, CY;Shi, S; | Investigation of multipotent postnatal stem cells from human periodontal ligament | Lancet (London, England) | 15246727 | BMSSCs were obtained from a commercially available resource (AllCells LLC, Berkeley, CA, USA). All primary cells used in this study were at 2-4 passages. For each experiment, the Save | Full Paper |
2004 | Griswold, IJ;Shen, LJ;La Rosée, P;Demehri, S;Heinrich, MC;Braziel, RM;McGreevey, L;Haley, AD;Giese, N;Druker, BJ;Deininger, MW; | Effects of MLN518, a dual FLT3 and KIT inhibitor, on normal and malignant hematopoiesis | Blood | 15242881 | Bone marrow mononuclear cells (5 × 104) obtained either commercially (n = 3) (AllCells, San Diego, CA) or from a volunteer donor (n = 1) were plated in methylcellulose media containing 50 ng/mL recombinant human (rh) stem cell factor, 10 ng/mL granul | Full Paper |
2004 | Geddis, AE;Kaushansky, K; | Megakaryocytes express functional Aurora-B kinase in endomitosis | Blood | 15130946 | stimulated donors were purchased (Cambrex, East Rutherford, NJ, or AllCells, Berkley, CA). Cells were thawed according to the manufacturer's directions and cultured for 10 to 14 days in StemPro (Invitrogen) containing recombinant human TPO (rhTPO; 50 | Full Paper |
2004 | Davis, BM;Humeau, L;Slepushkin, V;Binder, G;Korshalla, L;Ni, Y;Ogunjimi, EO;Chang, LF;Lu, X;Dropulic, B; | ABC transporter inhibitors that are substrates enhance lentiviral vector transduction into primitive hematopoietic progenitor cells | Blood | 15059841 | (> 98% purity) derived from adult bone marrow, adult granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood, and cord blood were purchased from AllCells (Berkeley, Save | Full Paper |
2004 | Lu, SJ;Li, F;Vida, L;Honig, GR; | CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic gene expression pattern | Blood | 14962900 | cells were obtained. Normal human BM was purchased from AllCells (Berkeley, CA); CD34+CD38- cells were purified as described previously in this paragraph. Figure 1 shows the fluorescence-activated cell sorter (FACS) profiles that define the gates for | Full Paper |
2004 | Morrissey, P;George, T;Basiji, D;Frost, K;Ortyn, W;Williams, D; | Cell Classification in Human Peripheral Blood Using the Amnis ImageStream® System. | Blood | Human peripheral blood was obtained from AllCells (Berkeley, CA). Whole blood was stained for 15 minutes on ice with FITC conjugated anti-CD45 mAb and PE conjugated anti-CD14 Save | Full Paper | |
2004 | Mahadevan, D;Spier, C;Croce, K;Miller, S;George, B;Riley, C;Warner, S;Grogan, T;Miller, T; | Gene Expression Profiling of Peripheral T-Cell Lymphoma (PTCL, NOS) and Comparison to Diffuse Large B-Cell Lymphoma (DLBCL). | Blood | Full Paper | ||
2004 | Masubuchi, N;May, RD;Atsumi, R; | A predictive model of human myelotoxicity using five camptothecin derivatives and the in vitro colony-forming unit granulocyte/macrophage assay | Clinical cancer research : an official journal of the American Association for Cancer Research | 15475463 | Cells were then washed in IMDM/5 and adjusted to the appropriate cell concentration. Human marrow samples were obtained from AllCells, LLC (Berkeley, CA) from donors that had given informed consent. The age of the 3 donors ranged from 20 to 45 years. | Full Paper |
2004 | Ibañez, V;Sharma, A;Buonamici, S;Verma, A;Kalakonda, S;Wang, J;Kadkol, S;Saunthararajah, Y; | AML1-ETO decreases ETO-2 (MTG16) interactions with nuclear receptor corepressor, an effect that impairs granulocyte differentiation | Cancer research | 15231665 | Retrovirus-containing supernatant was added to human cord blood CD34-positive cells (AllCells) in retronectin (TaKaRa)-coated plates. The cord blood cells were cultured in Iscove's Save | Full Paper |
2004 | Georgantas, RW;Tanadve, V;Malehorn, M;Heimfeld, S;Chen, C;Carr, L;Martinez-Murillo, F;Riggins, G;Kowalski, J;Civin, CI; | Microarray and serial analysis of gene expression analyses identify known and novel transcripts overexpressed in hematopoietic stem cells | Cancer research | 15231652 | The human CD34 + /CD38 − /Lin − cell subset, comprising ∼1-10% of the CD34 + cell population, contains few of the less primitive hematopoietic (lineage-committed) progenitor cells ( Save | Full Paper |
2004 | Humeau, LM;Binder, GK;Lu, X;Slepushkin, V;Merling, R;Echeagaray, P;Pereira, M;Slepushkina, T;Barnett, S;Dropulic, LK;Carroll, R;Levine, BL;June, CH;Dropulic, B; | Efficient lentiviral vector-mediated control of HIV-1 replication in CD4 lymphocytes from diverse HIV+ infected patients grouped according to CD4 count and viral load | Molecular therapy : the journal of the American Society of Gene Therapy | 15194057 | Peripheral blood from healthy donors was obtained from AllCells (Berkeley, CA, USA), and uninfected or HIV+ study subject PBMCs were isolated by Ficoll-Hypaque density gradient separation. Study subjects numbered J23 and higher and U01 had their bloo | Full Paper |
2004 | Davis, BM;Humeau, L;Dropulic, B; | In vivo selection for human and murine hematopoietic cells transduced with a therapeutic MGMT lentiviral vector that inhibits HIV replication | Molecular therapy : the journal of the American Society of Gene Therapy | 14759800 | progenitors were obtained from AllCells (Berkley, CA, USA) in accordance with an IRB protocol obtained by the company and were transduced and cultured in IMDM plus 1× BIT (Stem Cell Technologies, Vancouver, BC, Canada) in 25 ng/ml SCF, Flt-3L, and TP | Full Paper |
2004 | LeCouter, J;Zlot, C;Tejada, M;Peale, F;Ferrara, N; | Bv8 and endocrine gland-derived vascular endothelial growth factor stimulate hematopoiesis and hematopoietic cell mobilization | Proceedings of the National Academy of Sciences of the United States of America | 15548611 | Bv8 and endocrine-gland-derived VEGF (EG-VEGF), or prokineticins, are two highly related, secreted proteins that we previously described as selective angiogenic mitogens. Here we Save | Full Paper |
2004 | Su, AI;Wiltshire, T;Batalov, S;Lapp, H;Ching, KA;Block, D;Zhang, J;Soden, R;Hayakawa, M;Kreiman, G;Cooke, MP;Walker, JR;Hogenesch, JB; | A gene atlas of the mouse and human protein-encoding transcriptomes | Proceedings of the National Academy of Sciences of the United States of America | 15075390 | Tissue Preparation. Human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA), Clontech, AllCells (Berkeley, CA), Clonetics/BioWhittaker (Walk-ersville, MD), AMS Biotechnology (Abingdon, Oxfordshire, U.K.), and t | Full Paper |
2004 | Begovich, AB;Carlton, VE;Honigberg, LA;Schrodi, SJ;Chokkalingam, AP;Alexander, HC;Ardlie, KG;Huang, Q;Smith, AM;Spoerke, JM;Conn, MT;Chang, M;Chang, SY;Saiki, RK;Catanese, JJ;Leong, DU;Garcia, VE;McAllister, LB;Jeffery, DA;Lee, AT;Batliwalla, F;Remmers, E;Criswell, LA;Seldin, MF;Kastner, DL;Amos, CI;Sninsky, JJ;Gregersen, PK; | A missense single-nucleotide polymorphism in a gene encoding a protein tyrosine phosphatase (PTPN22) is associated with rheumatoid arthritis | American journal of human genetics | 15208781 | (GenBank accession number NM_015967) was determined by kinetic RT-PCR analysis by use of total RNA from sorted hematopoeitic cells (AllCells) and selected tissues (Clontech), as described elsewhere (Rogge et al. 2000). Data for additional tissues are | Full Paper |
2004 | Lu, X;Yu, Q;Binder, GK;Chen, Z;Slepushkina, T;Rossi, J;Dropulic, B; | Antisense-mediated inhibition of human immunodeficiency virus (HIV) replication by use of an HIV type 1-based vector results in severely attenuated mutants incapable of developing resistance | Journal of virology | 15194784 | Primary cell isolation, culture, transduction, and challenge. Peripheral blood from healthy donors was obtained from AllCells (Berkeley, Calif.), and lymphocytes were isolated by Ficoll-Hypaque density gradient separation. Cells were resuspended in b | Full Paper |
2004 | Chute, JP;Muramoto, G;Fung, J;Oxford, C; | Quantitative analysis demonstrates expansion of SCID-repopulating cells and increased engraftment capacity in human cord blood following ex vivo culture with human brain endothelial cells | Stem cells (Dayton, Ohio) | 14990859 | Purified human CB CD34+ cells were obtained from AllCells, LLC. (Berkeley, CA; http://www.allcells.com). HUBECs were subcultured at 1 × 105 cells/well in gelatin‐coated 6‐well plates (Costar; Cambridge, MA; http://www.corning.com) as previously descr | Full Paper |
2004 | Courtemanche, C;Huang, AC;Elson-Schwab, I;Kerry, N;Ng, BY;Ames, BN; | Folate deficiency and ionizing radiation cause DNA breaks in primary human lymphocytes: a comparison | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 14597554 | Normal human T lymphocytes were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% dialyzed Save | Full Paper |
2004 | Friedmann, E;Lemberg, MK;Weihofen, A;Dev, KK;Dengler, U;Rovelli, G;Martoglio, B; | Consensus analysis of signal peptide peptidase and homologous human aspartic proteases reveals opposite topology of catalytic domains compared with presenilins | The Journal of biological chemistry | 15385547 | Microarray Analysis—Human tissues, total RNA samples, and mRNA samples were obtained from Clinomics Biosciences, Inc., Clontech, AllCells, LLC, Clonetics/BioWhittaker, AMS Biotechnology, and the University of California, San Diego. The quality of all | Full Paper |
2004 | Courtemanche, C;Elson-Schwab, I;Mashiyama, ST;Kerry, N;Ames, BN; | Folate deficiency inhibits the proliferation of primary human CD8+ T lymphocytes in vitro | Journal of immunology (Baltimore, Md. : 1950) | 15322179 | Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen, Carlsbad, CA), supplemented with 10% dialyzed Save | Full Paper |
2004 | Chu, PC;Wu, J;Liao, XC;Pardo, J;Zhao, H;Li, C;Mendenhall, MK;Pali, E;Shen, M;Yu, S;Taylor, VC;Aversa, G;Molineaux, S;Payan, DG;Masuda, ES; | A novel role for p21-activated protein kinase 2 in T cell activation | Journal of immunology (Baltimore, Md. : 1950) | 15187108 | Freshly purified PBMC (AllCells, Berkeley, CA) were incubated at 37°C for 2 h to allow the monocytes to adhere to the tissue culture flask. The cells remaining in suspension were recovered and seeded at 106 cells/ml/ well in Yssel’s medium (Gemini Bi | Full Paper |
2004 | Chinnasamy, D;Fairbairn, LJ;Neuenfeldt, J;Treisman, JS;Hanson, JP;Margison, GP;Chinnasamy, N; | Lentivirus-mediated expression of mutant MGMTP140K protects human CD34+ cells against the combined toxicity of O6-benzylguanine and 1,3-bis(2-chloroethyl)-nitrosourea or temozolomide | Human gene therapy | 15319033 | Lentiviral vectors are capable of efficiently transducing nondividing and slowly dividing cells, including hematopoietic stem cells, resulting in stable integration and sustained transgene Save | Full Paper |
2004 | Lu, X;Humeau, L;Slepushkin, V;Binder, G;Yu, Q;Slepushkina, T;Chen, Z;Merling, R;Davis, B;Chang, YN;Dropulic, B; | Safe two-plasmid production for the first clinical lentivirus vector that achieves >99% transduction in primary cells using a one-step protocol | The journal of gene medicine | 15352069 | Normal human blood was obtained from AllCells (Berkely, CA, USA) under the auspices of an IRB-approved protocol supplied by AllCells. Peripheral blood mononuclear cells (PBMCs) Save | Full Paper |
2004 | Kubota, S;Kawata, K;Yanagita, T;Doi, H;Kitoh, T;Takigawa, M; | Abundant retention and release of connective tissue growth factor (CTGF/CCN2) by platelets | Journal of biochemistry | 15598883 | Lower panel: Comparison of the CTGF/CCN2 contents of platelets and other CTGF/CCN2-associated tissues. Total protein extracts of normal human skin and artery were obtained commercially (Cosmo Bio Co., Ltd., Tokyo, Japan), as were human platelets from | Full Paper |
2004 | Mashiyama, ST;Courtemanche, C;Elson-Schwab, I;Crott, J;Lee, BL;Ong, CN;Fenech, M;Ames, BN; | Uracil in DNA, determined by an improved assay, is increased when deoxynucleosides are added to folate-deficient cultured human lymphocytes | Analytical biochemistry | 15183762 | Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and 1% antibiotics (penicillin/streptavidin). To ensure | Full Paper |
2004 | Murray, B;Bennett, B;Sasaki, D; | Analysis of pharmacologic inhibitors of Jun N-terminal kinases | Methods in Enzymology | dom donor buffy coats from the San Diego Blood Bank (San Diego, CA). Neonatal cord blood is obtained from Advanced Bioscience Resources (Alameda, CA) or as selected CD4+ cells (AllCells, Foster City, CA) | Full Paper | |
2004 | Lu, SJ;Li, F;Vida, L;Honig, GR; | CD34CD38 Hematopoietic Precursors Derived from Human Embryonic | researchgate.net | of the total population, and a total of 2,840 CD34+ CD38- cells was obtained. Normal human BM was purchased from AllCells (AllCells, LLC, Berkeley, CA); CD34+ CD38- cells were purified as described above. Figure 1 shows the FACS profiles that define | Full Paper | |
2004 | Murmann, AE; | Topology of genes in mammalian cell nuclei with special emphasis on the MLL gene and its translocation partners | Thesis | ATCC = American Type Culture Collection, Manassas, VA. DSMZ = Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany. 3. Material 38 CD33+) were isolated by and purchased from AllCells Inc. (Berkeley, CA) | Full Paper | |
2004 | Mashiyama, ST; | Folate and other B-vitamins: Effects of deficiency on DNA-uracil content, chromosome damage, homocysteine, and interactions with gene polymorphisms | Thesis | Cell culture and proliferation Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and 1% antibiotics (penic | Full Paper | |
2003 | Winkler, DG;Sutherland, MK;Geoghegan, JC;Yu, C;Hayes, T;Skonier, JE;Shpektor, D;Jonas, M;Kovacevich, BR;Staehling-Hampton, K;Appleby, M;Brunkow, ME;Latham, JA; | Osteocyte control of bone formation via sclerostin, a novel BMP antagonist | EMBO J. | 14633986 | Mouse embryos (15.5 days old) were fixed in 4% phosphate-buffered formaldehyde pH 7.2 overnight, dehydrated and embedded in paraffin. Sections (5 µm thick) were prepared and in situ hybridization performed by Phylogeny, Inc. (Columbus, OH) using [35S | Full Paper |
2003 | Wang, T;Fan, L;Watanabe, Y;McNeill, PD;Moulton, GG;Bangur, C;Fanger, GR;Okada, M;Inoue, Y;Persing, DH;Reed, SG; | L523S, an RNA-binding protein as a potential therapeutic target for lung cancer | Br. J. Cancer | 12644826 | ur studies indicate that L523S may be a valuable addition to the repertoire of cancer-specific targets for the development of new immunotherapeutic and perhaps other therapeutic approaches. We thank Qualtek for their expert IHC analysis. We are grat | Full Paper |
2003 | DuHadaway, JB;Lynch, FJ;Brisbay, S;Bueso-Ramos, C;Troncoso, P;McDonnell, T;Prendergast, GC; | Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: diverse sites of nuclear expression and losses in prostate cancer | J. Cell. Biochem. | 12532338 | Search for more papers by this author. Frank J. Lynch QualTek Molecular Laboratories, Newtown, Pennsylvania. Search for more papers by this author. Shawn Brisbay Search for more papers by this author. Frank J. Lynch QualTek Molecular Laboratories, | Full Paper |
2003 | Tinkelenberg, BA;Fazzone, W;Lynch, FJ;Ladner, RD; | Identification of sequence determinants of human nuclear dUTPase isoform localization | Exp. Cell Res. | 12799180 | 15 min), DUT415 primary antibody incubation (25 min), biotinylated goat anti-mouse IgG secondary antibody (25 min), peroxidase-conjugated ABC-Elite (25 min, Vector Labs, Burlingame, CA, USA), and DAB (diaminobenzidine) chromogen (15 min, QualTek Mole | Full Paper |
2003 | Gibbs, WW; | Untangling the roots of cancer | Sci. Am. | 12840947 | SCIENCE PHOTO LIBRARY; FRANK LYNCH QualTek Molecular Laboratories; ANDREJS LIEPINS/SPL; CNRI/SPL; SPL 1. GROWTH EVEN IN THE ABSENCE OF NORMAL "GO" SIGNALS Most normal cells wait for an external message before dividing | Full Paper |
2003 | Wang, A;Clapper, J;Guderian, JA;Foy, TM;Fanger, GR;Retter, MW;Skeiky, YA; | A novel method for increasing the expression level of recombinant proteins | Protein Expr. Purif. | 12821330 | visualize antigen expression. Slides were counterstained with hematoxylin to visualize cell nuclei. We would like to acknowledge and thank Qualtek for immunohistochemistry results. FACS analysis. HEK293 cells were transiently | Full Paper |
2003 | Weigel-Kelley, KA;Yoder, MC;Srivastava, A; | Alpha5beta1 integrin as a cellular coreceptor for human parvovirus B19: requirement of functional activation of beta1 integrin for viral entry | Blood | 12907437 | Human bone marrow-derived highly purified CD71 + primary erythroid progenitor cells were purchased from AllCells (Berkeley, CA). Recombinant parvovirus B19-Luc vector stocks Save | Full Paper |
2003 | Francisco, JA;Cerveny, CG;Meyer, DL;Mixan, BJ;Klussman, K;Chace, DF;Rejniak, SX;Gordon, KA;DeBlanc, R;Toki, BE;Law, CL;Doronina, SO;Siegall, CB;Senter, PD;Wahl, AF; | cAC10-vcMMAE, an anti-CD30-monomethyl auristatin E conjugate with potent and selective antitumor activity | Blood | 12714494 | and goat-antihuman FITC were obtained from Jackson ImmunoResearch (West Grove, PA). Anti-CD30 mAb Ki-1 was from Accurate Chemicals (Westbury, NY). cBR96, a human chimeric IgG1κ (Seattle Genetics) was used as a chimeric, isotype-matched control. Fresh | Full Paper |
2003 | Glockner, A; | Optimal consistency protocols for replicated files | Eighth Annual International Phoenix Conference on Computers and Communications. 1989 Conference Proceedings | Placement continues until either allcells have been placed, or a number of iterations has beenperformed. If, during cell placement, a valid location for acell cannot be identified, the Save | Full Paper | |
2003 | Lee, J;Elkahloun, AG;Messina, SA;Ferrari, N;Xi, D;Smith, CL;Cooper, R;Albert, PS;Fine, HA; | Cellular and genetic characterization of human adult bone marrow-derived neural stem-like cells: a potential antiglioma cellular vector | Cancer research | 14695205 | RBCs from human adult (donors, 20-40 years old) bone marrow cells purchased from AllCells (Berkely, CA) were depleted by density gradient, and the remaining cells were plated in uncoated tissue culture plastic dishes. After 10 days of culture in 15% | Full Paper |
2003 | Yu, X;Zhan, X;D'Costa, J;Tanavde, VM;Ye, Z;Peng, T;Malehorn, MT;Yang, X;Civin, CI;Cheng, L; | Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cells | Molecular therapy : the journal of the American Society of Gene Therapy | 12788657 | Normal human placental/umbilical CB CD34+ cells, purchased from AllCells (San Mateo, CA), had been purified by immunomagnetic selection (Miltenyi Biotech, Auburn, CA) and were90% CD34+ upon reanalysis by FACS. CB CD34+ cells were cultured at 2.5-5 × | Full Paper |
2003 | Thach, DC;Shaffer, KM;Ma, W;Stenger, DA; | Assessing the feasibility of using neural precursor cells and peripheral blood mononuclear cells for detection of bioactive Sindbis virus | Biosensors & bioelectronics | 12782470 | Fresh normal PBMC were purchased from AllCells , LLC. The cells were received in Dubecco's PBS with 0.5% bovine serum albumin (BSA) and 2 mM EDTA. The cells were spun Save | Full Paper |
2003 | Russell, RS;Roldan, A;Detorio, M;Hu, J;Wainberg, MA;Liang, C; | Effects of a single amino acid substitution within the p2 region of human immunodeficiency virus type 1 on packaging of spliced viral RNA | Journal of virology | 14645555 | Allcells were supplemented with 10% fetal calf serum. Cord bloodmononuclear cells (CBMCs) were grown in RPMI 1640 supplemented with10% fetal calf serum and 20 U of interleukin- Save | Full Paper |
2003 | Cheng, L;Hammond, H;Ye, Z;Zhan, X;Dravid, G; | Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture | Stem cells (Dayton, Ohio) | 12634409 | BM samples collected from healthy and consenting human donors were purchased from AllCells Company (Berkeley, CA; http://www.allcells.com). The use of anonymous primary human cells for laboratory research was approved by the Internal Review Board of | Full Paper |
2003 | Hsu, DH;Paz, P;Villaflor, G;Rivas, A;Mehta-Damani, A;Angevin, E;Zitvogel, L;Le Pecq, JB; | Exosomes as a tumor vaccine: enhancing potency through direct loading of antigenic peptides | Journal of immunotherapy (Hagerstown, Md. : 1997) | 12973033 | CD14+ monocyte-enriched adherent cells from healthy donor leukopacks acquired from Allcells (Berkeley, CA) were cultured in ultrafiltered AIMV medium (Invitrogen) supplemented Save | Full Paper |
2003 | Hofstra, CL;Desai, PJ;Thurmond, RL;Fung-Leung, WP; | Histamine H4 receptor mediates chemotaxis and calcium mobilization of mast cells | The Journal of pharmacology and experimental therapeutics | 12626656 | Human HMC-1 and WEHI-3 cells were purchased from American Type Culture Collection (Manassas, VA). Human CD34 cord blood cells were from AllCells, LLC (Berkeley, CA). Basophil enrichment kit and serum-free medium were from Stem Cells Technologies (Van | Full Paper |
2003 | Greco, NJ;Lee, WR;Moroff, G; | Increased transmigration of G-CSF-mobilized peripheral blood CD34+ cells after overnight storage at 37 degrees C | Transfusion | 14617318 | Samples from UCSD and AllCells LLC were shipped overnight (ON) at 1 to 6C and utilized in experiments upon receipt the following morning (referred to as Day 1). PBPC samples from Save | Full Paper |
2003 | Scherer, A;Krause, A;Walker, JR;Sutton, SE;Serón, D;Raulf, F;Cooke, MP; | Optimized protocol for linear RNA amplification and application to gene expression profiling of human renal biopsies | BioTechniques | 12661160 | Human PBMCs were purchased from Allcells (Oakland, CA, USA). Cells were subjected to RNA purification with RNeasy® (Qiagen, Valencia, CA, USA). The RNA was eluted with 80 µL RNase-free water (Ambion, | Full Paper |
2003 | Onguru, O;Celasun, B;Gunhan, O; | Comparison of DNA ploidy and nuclear morphometric parameters with the conventional prognostic factors in transitional cell carcinomas | The Tohoku journal of experimental medicine | 12703658 | ThemeasurementofNA10in thisstudydemonstratedthemeannucleararea ofthebiggest10cellsamong the allcells measuredatthenucleussuspensionforeach case. Thus, Save | Full Paper |
2003 | Yamaguchi, H;Aridgides, LJ;Zeng, W;Osgood, C;Young, NS;Liu, JM; | Genetic and transcriptional analysis of spindle checkpoint genes in bone marrow failure patients | Blood cells, molecules & diseases | 12737950 | peripheral blood (PB)-MNCs was extracted using an RNAzol kit (Biotex Laboratories, Houston, TX) or Trizol (Life Technologies). Total RNA from purified T cell, B cell, and neutrophil fractions was purchased (AllCells, LLC, Berkeley, CA) | Full Paper |
2003 | Christensen, JL; | Mechanisms and mediators of hematopoietic stem cell fate | Thesis | Human bone marrow was obtained from normal donors (Allcells, Oakland, CA). Bone marrow was diluted in Hanks balanced salt solution, and 20ml was layered onto 15ml of ficoll (Ficoll-Paque PLUS (Amersham Phamacia Biotech), and centrifuged for 30 minute | Full Paper | |
2003 | Chu, P;Pardo, J;Zhao, H;Li, CC;Pali, E;Shen, MM;Qu, K;Yu, SX;Huang, BC;Yu, P;Masuda, ES;Molineaux, SM;Kolbinger, F;Aversa, G;de Vries, J;Payan, DG;Liao, XC; | Systematic identification of regulatory proteins critical for T-cell activation | Journal of biology | 12974981 | 1,599 of its open reading frame; and TRAC-1, base pairs 1-509 of its open reading frame. Culture and infection of primary T lymphocytes Commercially available primary blood mononuclear cells (PBMC; AllCells LLC, Berkeley, USA) were cultured in RPMI | Full Paper |
2003 | Knowles, S;Woods, R;McWhirter, J;McCanny, J; | Bit-level systolic arrays for IIR filtering | [1988] Proceedings. International Conference on Systolic Arrays | throughout this paper.,The application contains four main functions as follows:,1),startPlay,function calls,setup,to set the,dimension of all DOM elements with a class name of,allCells,;,2), Save | Full Paper | |
2003 | Chow, DC; | Effects of surface-immobilized stem cell factor on the differentiation and proliferation of hematopoietic cells | Thesis | gnetic cell separation system (Miltenyl Biotec GmbH, Bergisch Gladbach, Germany) (AllCells, Berkely, CA). Cells were cultured in the presence of soluble SCF (100 ng/ml) or surface-immobilized SCF in serum-free medium (XVIVO20. Cambrex Bio Science, Wa | Full Paper | |
2002 | Elias, PM;Ahn, SK;Denda, M;Brown, BE;Crumrine, D;Kimutai, LK;Kömüves, L;Lee, SH;Feingold, KR; | Modulations in epidermal calcium regulate the expression of differentiation-specific markers | J. Invest. Dermatol. | 12445203 | from Vector Laboratories (Burlingame, CA). Peroxidase activity was revealed with diaminobenzidine (QualTek Laboratories, Santa Barbara, CA), followed by counterstaining with methyl green. Omission of the first antibodies | Full Paper |
2002 | Xie, Z;Komuves, L;Yu, QC;Elalieh, H;Ng, DC;Leary, C;Chang, S;Crumrine, D;Yoshizawa, T;Kato, S;Bikle, DD; | Lack of the vitamin D receptor is associated with reduced epidermal differentiation and hair follicle growth | J. Invest. Dermatol. | 11851870 | Peroxidase activity was revealed with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followed by counterstaining with methyl green or hematoxylin. Omitting the first antibodies resulted in no signal, indicating the specificity o | Full Paper |
2002 | Fanger, GR;Houghton, RL;Retter, MW;Hendrickson, RC;Babcook, J;Dillon, DC;Durham, MD;Reynolds, LD;Johnson, JC;Carter, D;Fleming, TP;Roche, PC;Persing, DH;Reed, SG; | Detection of mammaglobin in the sera of patients with breast cancer | Tumour Biol. | 12499777 | Acknowledgments We thank Frank Lynch at Qualtek for immunohistochemistry efforts and expertise. Work was supported in part by USPHS grant CA76227 (TPF), and NIH grants CA-75794 (RLH) and CA- 86673 (DCD) References 1 Donegan W, Spratt J: Cancer of the | Full Paper |
2002 | Kumar, S;Hanning, CR;Brigham-Burke, MR;Rieman, DJ;Lehr, R;Khandekar, S;Kirkpatrick, RB;Scott, GF;Lee, JC;Lynch, FJ;Gao, W;Gambotto, A;Lotze, MT; | Interleukin-1F7B (IL-1H4/IL-1F7) is processed by caspase-1 and mature IL-1F7B binds to the IL-18 receptor but does not induce IFN-gamma production | Cytokine | 12096920 | Full Paper | |
2002 | Mulero, JJ;Boyle, BJ;Bradley, S;Bright, JM;Nelken, ST;Ho, TT;Mize, NK;Childs, JD;Ballinger, DG;Ford, JE;Rupp, F; | Three new human members of the lipid transfer/lipopolysaccharide binding protein family (LT/LBP) | Immunogenetics | 12185532 | The BPIL2 probe was derived from a 414-nt coding sequence between the primers 5'-CATTATTGCAAGTGAAGTCAAAGC-3' and 5'-ACCATGA- AGGGCTGGGACAAGATG-3'. QualTek Molecular labs (Santa Barbara, Calif.) performed the automated in situ hybridization us- ing a | Full Paper |
2002 | Zhao, Z;Yaar, R;Ladd, D;Cataldo, LM;Ravid, K; | Overexpression of A3 adenosine receptors in smooth, cardiac, and skeletal muscle is lethal to embryos | Microvasc. Res. | 11749073 | Page 4. tion, generating either a sense or an antisense strand template for in vitro transcription (with EcoRV or HindIII, respectively). In situ hybridization was performed by Dr. Adel Mikhail (Phylogeny, Inc., Columbus, OH) as described by Lyons et | Full Paper |
2002 | Hevehan, DL;Miller, WM;Papoutsakis, ET; | Differential expression and phosphorylation of distinct STAT3 proteins during granulocytic differentiation | Blood | 11861277 | Fresh G-CSF-mobilized peripheral blood CD34 + cells from normal donors were obtained from AllCells (Foster City, CA) within 24 hours after collection. The experimental design and Save | Full Paper |
2002 | Cui, Y;Golob, J;Kelleher, E;Ye, Z;Pardoll, D;Cheng, L; | Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells | Blood | 11781219 | Human G-CSF-mobilized peripheral blood or cord blood CD34+ cells were obtained from AllCells (San Mateo, CA). CD34+ cells were purified by immunomagnetic selection (Miltenyi Biotec, Auburn, CA) and then cryopreserved. The purity of CD34+ cells was us | Full Paper |
2002 | Tuttle, G;Fallahi, S;Abidi, A; | An 8 b CMOS vector A/D converter | 1993 IEEE International Solid-State Circuits Conference Digest of Technical Papers | The two pMOSFETsin allcells are diode connected, and the nMOSFET is biased by a globally-applied reference gate voltage. The input is then switched to the prompt vector X, and the Save | Full Paper | |
2002 | Kang, SJ;Cresswell, P; | Regulation of intracellular trafficking of human CD1d by association with MHC class II molecules | The EMBO journal | 11927549 | DCs were derived from monocytes as described (Sallusto and Lanzavecchia, 1994). Briefly, monocytes from peripheral blood mononuclear cells (8 × 108 cells) (AllCells, LLC, Berkeley, CA) were attached to tissue culture flasks in 20% FBS/RPMI 1640 twice | Full Paper |
2002 | Manz, MG;Miyamoto, T;Akashi, K;Weissman, IL; | Prospective isolation of human clonogenic common myeloid progenitors | Proceedings of the National Academy of Sciences of the United States of America | 12193648 | Bone Marrow and Cord Blood Samples. Fresh human bone marrow (donor age, 20-47 years) was obtained with informed consent from healthy volunteers (AllCells, Berkeley, CA), allogeneic bone marrow donors (Department of Bone Marrow Transplantation, Stanfo | Full Paper |
2002 | Mahmud, DL;G-Amlak, M;Deb, DK;Platanias, LC;Uddin, S;Wickrema, A; | Phosphorylation of forkhead transcription factors by erythropoietin and stem cell factor prevents acetylation and their interaction with coactivator p300 in erythroid progenitor cells | Oncogene | 11896584 | Primary human erythroid progenitors were derived by in vivo culture of CD34+ cells isolated from peripheral blood. Growth factor mobilized peripheral blood collected from normal donors was purchased from AllCells, LLC (Berkeley, CA, USA). These blood | Full Paper |
2002 | Yang, H;Miller, WM;Papoutsakis, ET; | Higher pH promotes megakaryocytic maturation and apoptosis | Stem cells (Dayton, Ohio) | 12110701 | Cultures were initiated with adult human CD34+ cells selected from apheresis products obtained from normal donors (AllCells; Berkeley, CA; http://www.allcells.com) or cancer patients (Northwestern Memorial Hospital, Chicago, IL) following stem cell m | Full Paper |
2002 | Kim, H;Whartenby, KA;Georgantas, RW;Wingard, J;Civin, CI; | Human CD34+ hematopoietic stem/progenitor cells express high levels of FLIP and are resistant to Fas-mediated apoptosis | Stem cells (Dayton, Ohio) | 11897874 | Human placental/umbilical cord blood (CB) CD34+ cell preparations, purified by immunomagnetic selection following manufacturer's instructions (Miltenyi Biotechnologies; Auburn, CA; http://www.miltenyibiotec.com) and then cryopreserved, were purchased | Full Paper |
2002 | Welm, AL;Timchenko, NA;Ono, Y;Sorimachi, H;Radomska, HS;Tenen, DG;Lekstrom-Himes, J;Darlington, GJ; | C/EBPalpha is required for proteolytic cleavage of cyclin A by calpain 3 in myeloid precursor cells | The Journal of biological chemistry | 12105198 | Bone marrow was isolated from both femurs of adult animals as described (25). CD33+ and CD15+ bone marrow cells were obtained from AllCells, L.L.C. skeletal muscle was harvested from adult animals as a control for calpain 3 Northern analysis | Full Paper |
2002 | Jacobs-Helber, SM;Abutin, RM;Tian, C;Bondurant, M;Wickrema, A;Sawyer, ST; | Role of JunB in erythroid differentiation | The Journal of biological chemistry | 11726656 | Growth factor-mobilized peripheral blood collected from normal donors was purchased from AllCells, LLC (Berkeley, CA). CD34 + cells were isolated from growth factor-mobilized Save | Full Paper |
2002 | Harrisson, F; | Cellular origin of the basement membrane in embryonic chicken/quail chimeras. | International Journal of Developmental Biology | such as Quailnucleoli, are not clear in allcells. The drawings show the place where the photomicrographs were taken. The dashed area indicates the locatIOn of the mesoblast. Save | Full Paper | |
2002 | Sharma, A; | The role of the hiwi gene in human hematopoiesis | Thesis | Louis, MO) and stained first with biotin-conjugated K6.1 (20 ug/ml). washed, and labeled with Miltenyi streptavidin-conjugated iron microbeads (Miltenyi Biotec) and selected as described above according to manufacturer's instructions. Cord blood (CB) | Full Paper | |
2001 | Afar, DE;Vivanco, I;Hubert, RS;Kuo, J;Chen, E;Saffran, DC;Raitano, AB;Jakobovits, A; | Catalytic cleavage of the androgen-regulated TMPRSS2 protease results in its secretion by prostate and prostate cancer epithelia | Cancer Res. | 11245484 | We thank Drs. Robert Eisenman, Owen Witte, and William Isaacs for helpful suggestions and critical reading of the manuscript; Frank Lynch and Page Erickson at QualTek Molecular Laboratories for their expertise in immunohistochemistry; and Celeste Jon | Full Paper |
2001 | Xu, J;Kalos, M;Stolk, JA;Zasloff, EJ;Zhang, X;Houghton, RL;Filho, AM;Nolasco, M;Badaró, R;Reed, SG; | Identification and characterization of prostein, a novel prostate-specific protein | Cancer Res. | 11245466 | Immunohistochemical Studies. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissues by QualTek Molecular Laboratories using the prostein-specific mouse monoclonal antibody 10E3- G4-D3. Chromosome Localization. The Gen | Full Paper |
2001 | Wang, T;Fan, L;Watanabe, Y;McNeill, P;Fanger, GR;Persing, DH;Reed, SG; | L552S, an alternatively spliced isoform of XAGE-1, is over-expressed in lung adenocarcinoma | Oncogene | 11753648 | An affinity purified anti-L552S polyclonal antibody was subjected to immunohistochemistry analysis by QualTek Molecular Laboratories (Santa Barbara, CA, USA). In all cases, four micron sections of formalin fixed, paraffin-embedded tissues were used. | Full Paper |
2001 | Lin, H;Ho, AS;Haley-Vicente, D;Zhang, J;Bernal-Fussell, J;Pace, AM;Hansen, D;Schweighofer, K;Mize, NK;Ford, JE; | Cloning and characterization of IL-1HY2, a novel interleukin-1 family member | J. Biol. Chem. | 11278614 | The anti-CD20 monoclonal antibody was purchased from DAKO (Carpenteria, CA), and the anti-Ki67 was obtained from Coulter Immunotech (Miami, FL). Immunohistochemistry was performed at QualTek Molecular Systems, Inc | Full Paper |
2001 | Kutty, RK;Kutty, G;Samuel, W;Duncan, T;Bridges, CC;El-Sherbeeny, A;Nagineni, CN;Smith, SB;Wiggert, B; | Molecular characterization and developmental expression of NORPEG, a novel gene induced by retinoic acid | J. Biol. Chem. | 11042181 | Immunoreactivity was detected by peroxidase staining using reagents from Dako Corp. (Carpinteria, CA). In Situ Hybridization. In situ hybridization was performed by Phylogeny Inc. following the procedure of Lyonset al. (18) as described before (19) | Full Paper |
2001 | Suerbaum, S;Lohrengel, M;Sonnevend, A;Ruberg, F;Kist, M; | Allelic diversity and recombination in Campylobacter jejuni | J. Bacteriol. | 11274115 | Different genes show network-like and tree-like phylogeny in C. jejuni. In order to further analyze the population structure of C. jejuni, we used split decomposition analysis. Split decomposition was originally described by Bandelt and Dress in 1992 | Full Paper |
2001 | Taylor, RE;Shows, KH;Zhao, Y;Pittler, SJ; | A PDE6A promoter fragment directs transcription predominantly in the photoreceptor | Biochem. Biophys. Res. Commun. | 11401494 | Mannheim). An outside commercial company (Qualtek Inc., CA) confirmed results obtained in-house using the same exper- imental probes as above with proprietary in situ hybridization re- agents and the following conditions | Full Paper |
2001 | Ladner, RD; | The role of dUTPase and uracil-DNA repair in cancer chemotherapy | Curr. Protein Pept. Sci. | 12374095 | await further investigation. ACKNOWLEDGEMENTS Special thanks is extended to Dr. Frank J. Lynch at QualTek Molecular Laboratories, Santa Barbara, CA, for expertise in immunohistochemistry. Special thanks are extended | Full Paper |
2001 | Gao, Z;Golob, J;Tanavde, VM;Civin, CI;Hawley, RG;Cheng, L; | High levels of transgene expression following transduction of long-term NOD/SCID-repopulating human cells with a modified lentiviral vector | Stem cells (Dayton, Ohio) | 11359950 | Normal human cord blood (CB) CD34+ cells were purchased from either AllCells (San Mateo, CA; http://www.purecell.com) or Poietics/BioWhittaker (Gaithersburg, MD; http://www.biowhittaker.com/). CD34+ cells were purified by immunomagnetic selection (Mi | |
2001 | Bonfoco, E;Li, E;Kolbinger, F;Cooper, NR; | Characterization of a novel proapoptotic caspase-2- and caspase-9-binding protein | The Journal of biological chemistry | 11350957 | Positively selected, CD19+ MACS-purified human B cells (AllCells, San Jose, CA) were resuspended at 1 × 106 cells/ml in Yssel's medium without antibody (Gemini Bioproducts, Calabasas, CA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT). | |
2001 | Alonso, A;Saxena, M;Williams, S;Mustelin, T; | Inhibitory role for dual specificity phosphatase VHR in T cell antigen receptor and CD28-induced Erk and Jnk activation | The Journal of biological chemistry | 11085983 | Human bone marrow was purchased from AllCells LLC (Foster City, CA). Bone marrow leukocytes were obtained by hypotonic lysis of the erythrocytes in water for 1 min followed by washing of the cells in RPMI medium. Peripheral blood lymphocytes (∼80% T | |
2001 | 西田耕介, ;高城敏美, ;木下進一, ;辻正, ; | 燃料電池・ガスタービン複合発電システムの性能解析 (熱工学, 内燃機関, 動力など) | 日本機械学会論文集 B 編 | 段目の電 のみに蒸気を投入する方式(1段目蒸気投入 方式,1stcel1steamadditionform)は,全 段の電 に蒸 気を投入する方式(全段蒸気投入方式,Allcells Steam additionform)よりも単位燃料 Save | ||
2001 | Mostafa, SS;Papoutsakis, ET;Miller, WM; | Oxygen tension modulates the expression of cytokine receptors, transcription factors, and lineage-specific markers in cultured human megakaryocytes | Experimental hematology | 11438210 | Cultures were initiated with adult human CD34+ cells selected from apheresis products obtained from normal donors mobilized with G-CSF (Poietics Technologies, Gaithersburg, MD, USA, and AllCells, Foster City, CA, USA). CD34+ cells were selected 1 day | |
2000 | Xu, J;Stolk, JA;Zhang, X;Silva, SJ;Houghton, RL;Matsumura, M;Vedvick, TS;Leslie, KB;Badaro, R;Reed, SG; | Identification of differentially expressed genes in human prostate cancer using subtraction and microarray | Cancer Res. | 10749139 | Truncated P503S (amino acid 113–241) was cloned into pET32b (Novagen) and expressed in E. coli as thioredoxin fusion proteins with a histidine tag. P503S was purified by nickel chromatography, digested with thrombin, and further purified by reverse-p | |
2000 | Kömüves, LG;Hanley, K;Lefebvre, AM;Man, MQ;Ng, DC;Bikle, DD;Williams, ML;Elias, PM;Auwerx, J;Feingold, KR; | Stimulation of PPARalpha promotes epidermal keratinocyte differentiation in vivo | J. Invest. Dermatol. | 10951268 | purchased from Vector (Burlingame, CA). Peroxidase activity was revealed with diaminobenzidine (QualTek Laboratories, Santa Barbara, CA), followed by counterstaining with methyl green. Omitting the first antibodies resulted | |
2000 | Johnson, LV;Ozaki, S;Staples, MK;Erickson, PA;Anderson, DH; | A potential role for immune complex pathogenesis in drusen formation | Exp. Eye Res. | 10865992 | ANDERSONa a Center for the Study of Macular Degeneration, Neuroscience Research Institute, University of California, Santa Barbara, CA 93106, USA, b QualTek Molecular Laboratories, Santa Barbara, CA 93111, USA (Received | |
2000 | Yeung, G;Mulero, JJ;McGowan, DW;Bajwa, SS;Ford, JE; | CD39L2, a gene encoding a human nucleoside diphosphatase, predominantly expressed in the heart | Biochemistry | 11041856 | the manufacturer. Automated in situ hybridization was performed by QualTek Molecular labs (Santa Barbara, CA) using a modified version of a previously published procedure (17). The Ventana Medical Systems, Inc. (Tucson | |
2000 | Kralchevsky, PA;Nagayama, K; | Capillary interactions between particles bound to interfaces, liquid films and biomembranes | Advances in colloid and interface science | 10768480 | This dissertation studies cellular rearrangements in tissues and attempts to establish the role of physical properties of cells, tissues and membranes in several biological phenomena. Save | |
2000 | Pettus, P; | Modeling Fecal Bacteria in Oregon Coastal Streams Using Spatially Explicit Watershed Characteristics | Portland State University | Pathogens, such as Escherichia coli and fecal coliforms, are causing the majority of water quality impairments in US, making up∼ 87% of this grouping's violations. Predicting and Save | ||
2000 | Mostafa, SS; | Effects of culture oxygen tension on human megakaryocytes: A phenotypic and mechanistic study | Thesis | All of the molecular studies were conducted using CD34+ cells from fresh apheresis products (~ 2% CD34+ cells) collected from G-CSF mobilized normal donors (Poietics Technologies, Gaithersburg, MD and AllCells, Foster City, CA). PB instead of BM cell | ||
1999 | Hubert, RS;Vivanco, I;Chen, E;Rastegar, S;Leong, K;Mitchell, SC;Madraswala, R;Zhou, Y;Kuo, J;Raitano, AB;Jakobovits, A;Saffran, DC;Afar, DE; | STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors | Proc. Natl. Acad. Sci. U.S.A. | 10588738 | Human tissues for RNA and protein analyses were obtained from the Human Tissue Resource Center at the University of California, Los Angeles; from the National Disease Research Interchange (Philadelphia); and from QualTek Molecular Labs (Santa Barbara | |
1999 | Kömüves, LG;Hanley, K;Jiang, Y;Katagiri, C;Elias, PM;Williams, ML;Feingold, KR; | Induction of selected lipid metabolic enzymes and differentiation-linked structural proteins by air exposure in fetal rat skin explants | J. Invest. Dermatol. | 10084306 | purchased from Vector (Burlingame, CA). Peroxidase activity was revealed with DAB substrate (QualTek Laboratories, Santa Barbara, CA) followed by counterstaining with methyl green. Omitting the first antibodies resulted | |
1999 | Hu, MC;Wang, YP;Mikhail, A;Qiu, WR;Tan, TH; | Murine p38-delta mitogen-activated protein kinase, a developmentally regulated protein kinase that is activated by stress and proinflammatory cytokines | J. Biol. Chem. | 10066767 | From the ‡ Department of Cell Biology, Amgen, Inc., Thousand Oaks, California 91320, ¶ Phylogeny, Inc., Columbus, Ohio 43212, and the ‖ Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030. Next Section. Abstrac | |
1999 | Widder, E;Johnsen, S;Bernstein, S;Case, J;Neilson, D; | Thin layers of bioluminescent copepods found at density discontinuities in the water column | Marine Biology | Communicated by NH Marcus, Tallahassee EA Widder (8) a S. Johnsen Harbor Branch Oceanographic Institution, Fort Pierce, Florida 34946, USA Fax: 001 (0)561 468-0757 e-mail: widder@hboi.edu SA Bernstein QualTek, Santa Barbara, California 93111, USA JF | ||
1999 | Payre, F;Vincent, A;Carreno, S; | ovo/svb integrates Wingless and DER pathways to control epidermis differentiation | Nature | 10421370 | Similar results were obtained in allcells of the embryonic ventral epidermis tested, using a variety of Gal4 drivers. The denticle production induced by ectopic svb expression does not Save | |
1998 | Weymann, R;Jannuzi, B;Lu, L;Bahcall, J;Bergeron, J;Boksenberg, A;Hartig, G;Kirhakos, S;Sargent, W;Savage, B;Schneider, D;Turnshek, D;Wolfe, A; | TheHubble Space TelescopeQuasar Absorption Line Key Project. XIV. The Evolution of Lyα Absorption Lines in the Redshift Intervalz= 0-1.5 | The Astrophysical Journal | We present the results of an analysis of the rate of evolution of the Lyα absorption lines in the redshift interval 0.0 to~ 1.5 based upon a sample of 987 Lyα absorption lines identified in Save | ||
1997 | Schmidt-Erfurth, U;Diddens, H;Birngruber, R;Hasan, T; | Photodynamic targeting of human retinoblastoma cells using covalent low-density lipoprotein conjugates | British journal of cancer | 9000598 | Firstly, the apoB/E receptor, which is present on allcells, is the main mechanism by which cells gain exogenous cholesterol and can be regulated up or down to attain cholesterol Save | |
1997 | Surinya, KH; | Heme biosynthesis in erythroid cells : transcriptional egulation of the human 5=aminolevulinate synthase 2 gene / a thesis submitted to the University of Adelaide for the degree of Doctor of Philosophy by Katharina Helen Surinya | Thesis | There are two isozymes for ALAS: a housekeeping form expressed in allcells (ALAS1), and an erythroid-specific form (ALAS2) responsible for the higher protoporphyrin synthesis in Save | ||
1996 | Kavanagh, K;Whittaker, PA; | Application of protoplast fusion to the nonconventional yeast | Enzyme and microbial technology | Microbio/. 1983. 129, 337 IL3377 Necas. 0.. Svoboda, A. and Havelkova, M. Mechanism of regen- eration of yeast protoplasts: Formation of the cell wall in %hixmc- ckcwon~~~~es pornbr. Folio. Bio[. 1968, 14, 80-85 Necan. 0. Cell wall synthesis in yeast | ||
1996 | Evers, BM;Zhou, Z;Dohlen, V;Rajaraman, S;Thompson, JC;Townsend, CM; | Fetal and neoplastic expression of the neurotensin gene in the human colon | Annals of surgery | 8651737 | This gene is present in allcells in the body, but, interestingly, it is differentially expressed. As shown in this study, it tends to be expressed in the fetal colon and in the adult small bowel. Save | |
1996 | Salinas, E;Abbott, LF; | A model of multiplicative neural responses in parietal cortex | Proceedings of the National Academy of Sciences of the United States of America | 8876244 | Thesetwo parameterswill either be the same for allcells, in which case we refer to them as m andb, orwill be chosen from Gaussian distributions with fixed means and standard Save | |
1996 | Nwoga, JC;Sniffen, JC;Peña-Rasgado, C;Kimler, VA;Rasgado-Flores, H; | Effect of pentachlorophenol on calcium accumulation in barnacle muscle cells | The Journal of physiology | 9011605 | Allcells were mounted in the experimental chamber in the presence of NaSWOCa (see Methods). Except for the case where the cells remained in NaSWOCa, cells were pre-incubated Save | |
1996 | Kineman, RD;Gettys, TW;Frawley, LS; | Role of guanine nucleotide-binding proteins, Gi alpha 3 and Gs alpha, in dopamine and thyrotropin-releasing hormone signal transduction: evidence for competition and commonality | The Journal of endocrinology | 8778223 | Given that SLO is an invasive agent and could result in cell lysis (approximately 1-2% of allcells lyse following SLO treatment), the RHPA allows for the evaluation ofPRL release from Save | |
1996 | Berry, RM;Berg, HC; | Torque generated by the bacterial flagellar motor close to stall | Biophysical journal | 8968619 | ||
1996 | Goodman, MB;Art, JJ; | Positive feedback by a potassium-selective inward rectifier enhances tuning in vertebrate hair cells | Biophysical journal | 8804626 | ||
1996 | Laughlin, MJ;Christiansen, NP;Herzig, GP;Blumenson, L;Bonney, D;Stewart, CC; | CD34 progenitor cell subset analyses in normal human bone marrow and marrow harvested after intermediate-dose chemotherapy | Cytometry | 8979021 | CD34 + cells exhibiting high side scatter, R8 (to include allcells to theright ofR1)were >95% basophils(Fig. 2f). Most mature basophils exhibit low side scatter because their granules Save | |
1995 | Wang, XJ;Golomb, D;Rinzel, J; | Emergent spindle oscillations and intermittent burst firing in a thalamic model: specific neuronal mechanisms | Proceedings of the National Academy of Sciences of the United States of America | 7777551 | The histograms were obtained by computing the mean bursting rates of allcells averaged over a timeinterval of 10 s. (D) Normalized interburst interval (IBI) histogram (bin = 10 ms) Save | |
1995 | Nooter, K;Boersma, AW;Oostrum, RG;Burger, H;Jochemsen, AG;Stoter, G; | Constitutive expression of the c-H-ras oncogene inhibits doxorubicin-induced apoptosis and promotes cell survival in a rhabdomyosarcoma cell line | British journal of cancer | 7880739 | At 1 JM doxorubicin - a concentration that gives more than 4 log cell kill in a clonogenic assay on the R2NEO cells (Figure 3) - 96 h after drug incubation practically allcells were Save | |
1995 | Hofstadler, SA;Swanek, FD;Gale, DC;Ewing, AG;Smith, RD; | Capillary electrophoresis-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for direct analysis of cellular proteins | Analytical chemistry | 7741217 | The initial cell population has a significant spatial distribution, and it is unlikely that allcells lysed simultaneously, leading to band dispersion greater than that due to the initial spatial Save | |
1995 | Juckett, MB;Balla, J;Balla, G;Jessurun, J;Jacob, HS;Vercellotti, GM; | Ferritin protects endothelial cells from oxidized low density lipoprotein in vitro | The American journal of pathology | 7677189 | In allcells, ferritin synthesis is tightly coupled with the intracellular iron concentration, which presumably prevents expansion of a potentially damaging free iron pool. We have found that Save | |
1995 | Preudhomme, C;Lepelley, P;Lovi, V;Zandecki, M;Cosson, A;Fenaux, P; | T-cell acute lymphoblastic leukemia occurring in the course of B cell chronic lymphocytic leukemia: a case report | Leukemia & lymphoma | 8535207 | In the six cases studied, similar immunoglobulin chain isotypes were found in the CLL and ALLcells, while identical cytogenetic abnormalities were present in CLL and ALL in the Save | |
1994 | Uckun, F;Downing, J;Chelstrom, L;Gunther, R;Ryan, M;Simon, J;Carroll, A;Tuel-Ahlgren, L;Crist, W; | Human t(4;11)(q21;q23) acute lymphoblastic leukemia in mice with severe combined immunodeficiency | Blood | |||
1994 | Shikama, N;Ackermann, R;Brack, C; | Protein synthesis elongation factor EF-1 alpha expression and longevity in Drosophila melanogaster | Proceedings of the National Academy of Sciences of the United States of America | 8183891 | Therefore, regulation of protein synthesis can beconsidered as one key homeostatic function common to allcells. A decrease in the rate ofprotein synthesis with advancing age has been Save | |
1994 | Yao, A;Rubin, H; | A critical test of the role of population density in producing transformation | Proceedings of the National Academy of Sciences of the United States of America | 8052648 | In a competentpopulation, allcells have the capacityto produce some transformed foci if kept under the constraint of confluence and/or reduced serum concentration for extended Save | |
1994 | Ingenbleek, Y;Young, V; | Transthyretin (prealbumin) in health and disease: nutritional implications | Annual review of nutrition | 7946531 | the fteld of both thyroid hormones (175, 176), whose three speciftc carrier proteins act as buffering reservoirs, thereby allowing the uniform distribution of the horн mones among allcells Save | |
1994 | Ferreira, GC; | Mammalian ferrochelatase. Overexpression in Escherichia coli as a soluble protein, purification and characterization | The Journal of biological chemistry | 8308010 | dance of ferrochelatase in mitochondria and its difficulty of Although ferrochelatase is present in allcells, it is predomi- solubilization from the membrane environment (1,2). In this nantly Save | |
1994 | McDonnell, DP;Goldman, ME; | RU486 exerts antiestrogenic activities through a novel progesterone receptor A form-mediated mechanism | The Journal of biological chemistry | 8163495 | In the pri- suggests that estrogen receptor-mediated activation of tranmate uterus, for instance, in addition to functioning an an- scription is not identical in allcells. Since PR-A is not Save | |
1994 | Skiba, PJ;Keesler, GA;Tabas, I; | Interferon-gamma down-regulates the lipoprotein(a)/apoprotein(a) receptor activity on macrophage foam cells. Evidence for disruption of ligand-induced receptor recycling by interferon-gamma | The Journal of biological chemistry | 8083207 | Allcells were then assayed for 5 h 3 (in the absence IFN-y) for lZ5I-r-apo(a) degradation. C,resident mouse peritoneal macrophages werepreincubated for 3 days in DMEM, 10%LPDS, Save | |
1994 | Cazenave, C;Frank, P;Toulme, JJ;Büsen, W; | Characterization and subcellular localization of ribonuclease H activities from Xenopus laevis oocytes | The Journal of biological chemistry | 7929207 | to be primarily concentrated in the germinal drawn from the various attempts to characterize these envesicle, but around of this activity was detected 5% in the zymes further: allcells Save | |
1994 | Carrel, F;Dharmawardhane, S;Clark, AM;Powell-Coffman, JA;Firtel, RA; | Spatial and temporal expression of the Dictyostelium discoideum G alpha protein subunit G alpha 2: expression of a dominant negative protein inhibits proper prestalk to stalk differentiation | Molecular biology of the cell | 8186466 | Using the full-length promoter, aggregates showed uniform staining during aggregation and mound formation, suggesting that Ga2 is expressed in allcells at this stage of development, Save | |
1994 | Aoyagi, M;Furusawa, S;Waga, K;Tsunogake, S;Shishido, H; | Suppression of normal hematopoiesis in acute leukemia: effect of leukemic cells on bone marrow stromal cells and hematopoietic progenitor cells | Internal medicine (Tokyo, Japan) | 7949632 | Effects ofleukemic cells (LC) onbone marrowstromal cells and myeloid progenitor cells (CFUC) were studied in vitro, using LClines with different lineage characteristics. LCand/or LC- Save | |
1994 | Van Hamme, LJ; | Associative and statistical accounts of cue competition in causality judgments | Thesis | This expression equ ally utilizes the inform ation in allcells of the ta b le and re p re sen ts one measure of the tru e contingency between the even ts. Although developed w ith in qu ite Save | ||
1993 | Kohl, NE;Mosser, SD;deSolms, SJ;Giuliani, EA;Pompliano, DL;Graham, SL;Smith, RL;Scolnick, EM;Oliff, A;Gibbs, JB; | Selective inhibition of ras-dependent transformation by a farnesyltransferase inhibitor | Science (New York, N.Y.) | 8316833 | Apparently, lovastatin's growth-inhibitory effects involve multiple mevalonate-dependent pathways that are required for allcells to growand divide. The specificity observed with L- Save | |
1993 | Campana, D;Coustan-Smith, E;Manabe, A;Buschle, M;Raimondi, SC;Behm, FG;Ashmun, R;Aricò, M;Biondi, A;Pui, CH; | Prolonged survival of B-lineage acute lymphoblastic leukemia cells is accompanied by overexpression of bcl-2 protein | Blood | 8427984 | Overexpression of bcl-2 delays the onset of apoptosis in lymphohematopoietic cells. We measured levels of bcl-2 protein in normal and leukemic human B-cell progenitors with a Save | |
1993 | Ruiz-Gómez, M;Ghysen, A; | The expression and role of a proneural gene, achaete, in the development of the larval nervous system of Drosophila | The EMBO journal | 8458326 | The late expression is abnormal in that most or allcells of the clusters shift to, and stay at, the high level of expression that is normally observed only in the progenitor cell. We conclude Save | |
1993 | Rice, NR;Ernst, MK; | In vivo control of NF-kappa B activation by I kappa B alpha | The EMBO journal | 8223478 | Whatever the basis for IxBct's instability in the cell lines examined here, there is no apriori reason to think that allcells have such unstable IxBa.For example, if the hypothetical kinase Save | |
1993 | Camplejohn, RS;Brock, A;Barnes, DM;Gillett, C;Raikundalia, B;Kreipe, H;Parwaresch, MR; | Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells | British journal of cancer | 7682430 | There is considerable interest in immunohistochemical markers of proliferation which are suitable for use on routinely fixed clinical material. The novel proliferation-associated antibody Save | |
1993 | Art, JJ;Fettiplace, R;Wu, YC; | The effects of low calcium on the voltage-dependent conductances involved in tuning of turtle hair cells | The Journal of physiology | 8308720 | Allcells tuned to very low frequencies also possessed an inward rectifier (see records in Fig. C) revealed with hyperpolarizing pulses negativeto the K+ equilibrium potential. An inward Save | |
1993 | Cociancich, S;Ghazi, A;Hetru, C;Hoffmann, JA;Letellier, L; | Insect defensin, an inducible antibacterial peptide, forms voltage-dependent channels in Micrococcus luteus | The Journal of biological chemistry | 7690029 | There are at least two possible explanations for this observation: either allcells are not hit by defensin, or they are all hit, but areonly partially depleted of K'. A partial depletion of K' Save | |
1993 | Masumoto, A;Hemler, ME; | Multiple activation states of VLA-4. Mechanistic differences between adhesion to CS1/fibronectin and to vascular cell adhesion molecule-1 | The Journal of biological chemistry | 7677996 | We examined the effects of a stimulatory anti-beta 1 mAb (TS2/16) and different divalent cations on VLA-4-mediated cell adhesion to vascular cell adhesion molecule-1 (VCAM-1), to the Save | |
1993 | Stauffer, P;Zhao, H;Luby-Phelps, K;Moss, R;Star, R;Muallem, S; | Gap junction communication modulates [Ca2+]i oscillations and enzyme secretion in pancreatic acini. | The Journal of biological chemistry | These oscillations stopped in allcells on stopping stimulationindependent of the number or the length of the focal stimulations. Global CCKJ stimulation of the same acinus also induced [ Save | ||
1993 | Sinha, SK;Todd, SC;Hedrick, JA;Speiser, CL;Lambris, JD;Tsoukas, CD; | Characterization of the EBV/C3d receptor on the human Jurkat T cell line: evidence for a novel transcript | Journal of immunology (Baltimore, Md. : 1950) | 8390533 | (28) demonstrated that HPB-ALLcells coexpress CR2 and CRl and that a portion of these two C3 receptors are associated in the form of CRl/CR2 complexes in the cell membrane. Save | |
1993 | Panzeter, PL;Ringer, DP; | DNA precursors are channelled to nuclear matrix DNA replication sites | The Biochemical journal | 8352746 | Precursors injected into the bloodstream do not have equal access to allcells, especially during the short pulse times used in this study. Many replicating cells will not see the precursor Save | |
1993 | D'Andrea, RJ;Stratmann, R;Lehner, CF;John, UP;Saint, R; | The three rows gene of Drosophila melanogaster encodes a novel protein that is required for chromosome disjunction during mitosis | Molecular biology of the cell | 8305737 | (I) An embryo during cellularization showing lack of transcripts in allcells except pole (presumptive germ) cells. 0) Cellular blastodern at higher magnification showingpunctate staining Save | |
1993 | Dorocicz, IR;Williams, PM;Redfield, RJ; | The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competence | Journal of bacteriology | 8226661 | This cAMP-independent event must be induced in allcells when cultures are transferred to MIV, but it may also occur spontaneously in the small fraction of cells that develop Save | |
1993 | Humm, J;Chin, L; | A Model of Cell Inactivation by Alpha-Particle Internal Emitters | Radiation Research | The bottom survival curve is produced by 50% of the sources bound uniformly to allcells. The middle curve is for 50% of the source decays occurring on half of the cells for type A Save | ||
1993 | Mukhtar, DD;Stewart, IJ; | Observations of the surface morphology of cultured granulated metrial gland cells | Journal of anatomy | 8226296 | The features described above were found on GMG cells at all stages of culture examined and on allcells from all stages of pregnancy examined. However, it was noticeable that the Save | |
1993 | Edelsbrunner, H;Seidel, R;Sharir, M; | On the Zone Theorem for Hyperplane Arrangements | SIAM Journal on Computing | We define Zone(b; H) to be the set of allcells in the arrangement A(H) that intersect the hypewlane b, and the cells were defined to be open sets. Previously the zone used to be defined Save | ||
1993 | Ohno, H;Inoue, T;Akasaka, T;Okumura, A;Miyanishi, S;Ohashi, I;Kikuchi, M;Masuya, M;Amano, H;Imanaka, T; | Acute lymphoblastic leukemia associated with a t(1;19)(q23;p13) in an adult | Internal medicine (Tokyo, Japan) | 8286840 | ThePCRanalysis of the present patient revealed the samemolecular evidence of E2A/PBX1 fusion as found in childhood ALLcells. Thus, molecular analysis of the E2A/PBX1fusion gene Save | |
1993 | Herrmann, H;Karsch, F; | LARGE SCALE COMPUTATIONAL PHYSICS ON MASSIVELY PARALLEL COMPUTERS | Large Scale Computational Physics on Massively Parallel Computers | time matrix vector multiplication 19.4 sec sum over allcells 0.012 sec Time for allcells to allcells communication. Save | ||
1993 | Shamim, KM; | Frog retinal ganglion cells. Morphological and spatial analyses | Thesis | For an auto-correlogram, allcells of the same type as the central reference cell are picked up (asshown schematically in A3) while a cross-correlogram locates all cellsof another type in Save | ||
1992 | Kim, CY;Giaccia, AJ;Strulovici, B;Brown, JM; | Differential expression of protein kinase C epsilon protein in lung cancer cell lines by ionising radiation | British journal of cancer | 1329908 | The effect of ionising radiation on the regulation of gene and protein expression is complex. This study focuses on the translational regulational of the epsilon isoform of protein kinase C Save | |
1992 | Rooney, IA;Davies, A;Morgan, BP; | Membrane attack complex (MAC)-mediated damage to spermatozoa: protection of the cells by the presence on their membranes of MAC inhibitory proteins | Immunology | 1374057 | On fluorescence microscopy, allcells stained brightly with both BRIC 229 and A 35 anti-CD59 antibodies (Fig. 2a-c). The pattern ofstaining vfaried (eg Fig. Save | |
1992 | Smedts, F;Ramaekers, F;Troyanovsky, S;Pruszczynski, M;Robben, H;Lane, B;Leigh, I;Plantema, F;Vooijs, P; | Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia | The American journal of pathology | 1372156 | A completely filled bar indicates an intensely positive reaction in allcells. An open bar indicates that all cells are negative, whereas a small square indicates that only afew cells Save | |
1992 | Hohmann, HP;Remy, R;Aigner, L;Brockhaus, M;van Loon, AP; | Protein kinases negatively affect nuclear factor-kappa B activation by tumor necrosis factor-alpha at two different stages in promyelocytic HL60 cells | The Journal of biological chemistry | 1730737 | Allcells were used after reaching near confluence or a density of 1-1.5 X lo6 cells/ml of culture medium. Preparation of Nuclear Extracts and Electrophoretic Mobility Shift Assays (EMSA) Save | |
1992 | Kirby, MS;Sagara, Y;Gaa, S;Inesi, G;Lederer, WJ;Rogers, TB; | Thapsigargin inhibits contraction and Ca2+ transient in cardiac cells by specific inhibition of the sarcoplasmic reticulum Ca2+ pump | The Journal of biological chemistry | 1320000 | Recently, it has been reported that in allcells examined, a common response evoked by thapsigargin is a rapid increase in cytosolic [Ca2+],possibly resulting from the mobilization of Save | |
1992 | Bednarczyk, J;Szabo, M;McIntyre, B; | Post-translational processing of the leukocyte integrin alpha 4 beta 1. | Journal of Biological Chemistry | Endo F digestion of a4 and B1 from HPB-ALLcells. and and IL-2-dependent T lymphocytes cultured for 43 days (Fig. HPB-ALL cells were 'ZsII-cell-surface-labeled lysed in Nonidet P4). Save | ||
1992 | García-Alvarado, JS;Rodriguez, MA;Labbé, RG; | Influence of elevated temperature on starch hydrolysis by enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens type A | Applied and environmental microbiology | 1371660 | The suspension was sonicated for 45 s with 60-s cooling periods until essentially allcells were disrupted. The sample was thencentrifuged at 10,000 x g for 30 min, and Save | |
1992 | Szabà, G;Pine, PS;Weaver, JL;Kasari, M;Aszalos, A; | Epitope mapping by photobleaching fluorescence resonance energy transfer measurements using a laser scanning microscope system | Biophysical journal | 1380319 | ||
1992 | Shatz, CJ; | The developing brain | Scientific American | 26269896 | Allcells on the array fired with in about five seconds of one another, in a predictable and rhythmic pattern. The bursts of action potentials lasted several seconds and were followed by Save | |
1991 | Bejar, I; | A GENERATIVE APPROACH TO PSYCHOLOGICAL AND EDUCATIONAL MEASUREMENT | ETS Research Report Series | in this taxonomy could be assessed by the degree to which the psychometric parameters from a given cell are well-predicted and the within-cell residuais are constant across allcells. Save | ||
1991 | Burton, GF;Kosco, MH;Szakal, AK;Tew, JG; | Iccosomes and the secondary antibody response | Immunology | 1879876 | The resulting preparations were observed microscopically to ensure that allcells were disrupted. Antigen concentration was determined by using radiolabelled antigen and assessing Save | |
1991 | Hudson, TH;Grillo, FG; | Brefeldin-A enhancement of ricin A-chain immunotoxins and blockade of intact ricin, modeccin, and abrin | The Journal of biological chemistry | 1917980 | This receptor interacts with transport of newly synthesized plasma membrane glycoproproteoglycans in allcells studied the Gal bindingsitesontheB-chain of ricin and would teins, Save | |
1991 | Novy, RE;Lin, JL;Lin, JJ; | Characterization of cDNA clones encoding a human fibroblast caldesmon isoform and analysis of caldesmon expression in normal and transformed cells | The Journal of biological chemistry | 1885618 | Allcells were grownin Dulbecco's modified Eagle's mediumcontaining 10% fetal calf serum and maintained in a 37 "C humidified incubator with 95% air and 5% CO,. Western Blot and Save | |
1991 | Järveläinen, HT;Kinsella, MG;Wight, TN;Sandell, LJ; | Differential expression of small chondroitin/dermatan sulfate proteoglycans, PG-I/biglycan and PG-II/decorin, by vascular smooth muscle and endothelial cells in culture | The Journal of biological chemistry | 1744124 | ||
1991 | van Praag, MC;Boom, BW;van Hees, CL;Vermeer, BJ;Scheffer, E; | Classical and ulcerative lichen planus with plasma cell infiltrate | Archives of dermatology | 1990998 | Plasma cells accounted for 60% to 70% of allcells. The infiltrate invaded the epidermis causing apoptoticand vacuolar degeneration of basal and parabasal cells (Fig 2). The lesions of Save | |
1991 | Humbert, P;Agache, P; | Acquired ichthyosis: a new cutaneous marker of autoimmunity | Archives of dermatology | 1990997 | Plasma cells accounted for 60% to 70% of allcells. The infiltrate invaded the epidermis causing apoptoticand vacuolar degeneration of basal and parabasal cells (Fig 2). The lesions of Save | |
1991 | Hall, AJ; | Steady size distributions in cell populations : a thesis presented in partial fulfilment of the requirements for the degree of Doctor Philosophy in Mathematics at Massey University | Thesis | Special cases are also considered where allcells with the same birth-size divide at the ), and allcells divide into equal-sized daughters, then we obtain a functional differential equation. Save | ||
1990 | Finn, PW;Kara, CJ;Van, TT;Douhan, J;Boothby, MR;Glimcher, LH; | The presence of a DNA binding complex correlates with E beta class II MHC gene expression | The EMBO journal | 2328725 | Even though the three motifs do not enhance CAT activity when tested individually, allcells that we have tested contain factors that bind to W, X and Y (Figure 4). Save | |
1990 | Yayon, A;Klagsbrun, M; | Autocrine transformation by chimeric signal peptide-basic fibroblast growth factor: reversal by suramin | Proceedings of the National Academy of Sciences of the United States of America | 2164679 | Most if not allcells possess FGF receptors, which are phosphorylated andinternalized upon binding of FGF. A 130-kDa high-affinity FGF receptor containing an Save | |
1990 | Berger, JD;Rahemtullah, S; | Commitment to autogamy in Paramecium blocks mating reactivity: implications for regulation of the sexual pathway and the breeding system | Experimental cell research | 2298253 | From this point on, allcells become committed to autogamy at a cell density that is lover than that required for the onset of mating reactivity. The cell cycle experiments discussed above Save | |
1990 | Dixon, CJ;Woods, NM;Cuthbertson, KS;Cobbold, PH; | Evidence for two Ca2(+)-mobilizing purinoceptors on rat hepatocytes | The Biochemical journal | 2386488 | Other than in the total absence of receptors of both subsets, allcells will respond to ATP.We propose that the cells responding to ATP with transients of short duration lack receptors of Save | |
1989 | Malinow, R;Schulman, H;Tsien, RW; | Inhibition of postsynaptic PKC or CaMKII blocks induction but not expression of LTP | Science (New York, N.Y.) | 2549638 | Bath-applied H-7 can suppress the expression of LTP (3), although it is not known whether the locus of action is pre- or postsynaptic, since it has access to allcells in the preparation. To Save | |
1989 | Johnson, JP;Stade, BG;Holzmann, B;Schwäble, W;Riethmüller, G; | De novo expression of intercellular-adhesion molecule 1 in melanoma correlates with increased risk of metastasis | Proceedings of the National Academy of Sciences of the United States of America | 2643120 | Identical restriction fragments were observed in allcells analyzed, including four autologous melanoma/B-cell pairs. The P3.58/ICAM-1 gene, therefore, does not seem to be polymorphic Save | |
1989 | Knust, B;Brüggemann, U;Doerfler, W; | Reactivation of a methylation-silenced gene in adenovirus-transformed cells by 5-azacytidine or by E1A trans activation | Journal of virology | 2473219 | Of course, it was not possible to show that allcells had been transfected equally efficiently. Reactivation of E2Atranscription might have occurred only in a subpopulation of the HE3 cells. Save | |
1989 | Celis, E;Karr, RW; | Presentation of an immunodominant T-cell epitope of hepatitis B surface antigen by the HLA-DPw4 molecule | Journal of virology | 2463381 | Thus, it appears that in the heterogeneous PBMC population not allcells express DPw4 molecules and that only thosethat do can present HBsAg (or Sld) to the Th. The inhibitory effect Save | |
1989 | Navaratnam, V;Woodward, JM;Skepper, JN; | Specific heart granules and natriuretic peptide in the developing myocardium of fetal and neonatal rats and hamsters | Journal of anatomy | 2532637 | Within the fetal atria, almost allcells seem capable of ANP synthesis and the concentration of ANP immunoreactivity increases sharply with development even after birth. Recent light Save | |
1988 | Nicolson, GL;Dulski, KM;Trosko, JE; | Loss of intercellular junctional communication correlates with metastatic potential in mammary adenocarcinoma cells | Proceedings of the National Academy of Sciences of the United States of America | 3422438 | , and the gene coding for polyoma virus middlesized tumor (middle T) antigen can decrease intercellular junctional communication in certain cellsystems (13-16), although not allcells Save | |
1988 | Hall, JG; | Review and hypotheses: somatic mosaicism: observations related to clinical genetics | American journal of human genetics | 3052049 | Conditions such as McCune-Albright syndrome may well represent lethal human mutations when present in allcells, but, by isolating the tissue involved, it may be possible to compare Save | |
1988 | Siebenlist, U;Bressler, P;Kelly, K; | Two distinct mechanisms of transcriptional control operate on c-myc during differentiation of HL60 cells | Molecular and cellular biology | 3162550 | These sites occur in allcells which we have analyzed (46, 47). In previous reports (3, 20, 22, 28, 29), only partial and even somewhat conflicting information on the chromatin structure of Save | |
1988 | Adány, R;Glukhova, MA;Kabakov, AY;Muszbek, L; | Characterisation of connective tissue cells containing factor XIII subunit a | Journal of clinical pathology | 2893813 | Though the expression of certain antigens might be lost at culture, allcells stained for the fibroblast markers IIIG1O and alkaline phosphatase lack this antigen. This clearly suggests that Save | |
1988 | Kim, T;Zeigler, B; | The class kernel-models in DEVS-scheme: a hypercube architecture example | ACM SIGSIM Simulation Digest | set-allcells-positions, which generates 2 n number of n-tuple coordinates to be attached to corresponding components before locating them in their positions. The method set-allcells- Save | ||
1988 | Reno, PW; | Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions | Coastal and Estuarine | N ear ly identic al reactions were found in kidney (Figure 2?) and liver (data not shown) -allcells seeme d to conta in IPN antige n. However, gastrointestin al tra ct tissue, especiallyt he Save | ||
1987 | Art, JJ;Fettiplace, R; | Variation of membrane properties in hair cells isolated from the turtle cochlea | The Journal of physiology | 2443666 | The shape of the current-voltage relationship was found to be similar in allcells, being flat at hyperpolarized levels and increasing its slope with depolarization through the resting Save | |
1987 | Segarini, PR;Roberts, AB;Rosen, DM;Seyedin, SM; | Membrane binding characteristics of two forms of transforming growth factor-beta | The Journal of biological chemistry | 2889729 | Essentially allcells have high affinity receptors that specifically bind TGF-/3 (Kdvalues of 1-60 pM, Refs. 11, 12, 14, and 15). The receptor number is tissue- and species-specific and Save | |
1987 | Richardson, A;Butler, JA;Rutherford, MS;Semsei, I;Gu, MZ;Fernandes, G;Chiang, WH; | Effect of age and dietary restriction on the expression of alpha 2u-globulin | The Journal of biological chemistry | 2442168 | regulation of gene expression represents one site regulation of common to allcells in a living organism, and changes in gene 24. expression can have profound effect at both the a Save | |
1987 | ROBERTS, D; | A zonal method for modeling 3-D aircraft flow fields with jet plume effects | 19th AIAA, Fluid Dynamics, Plasma Dynamics, and Lasers Conference | CON is then updated, and the cycle 18 repeated until the error in continuity falls below a prescribed tolerance for allcells . The relaxation factor, 0, Save | ||
1986 | Greaves, MF; | Differentiation-linked leukemogenesis in lymphocytes | Science (New York, N.Y.) | 3535067 | us the monodonal antibody-defined makers T9 and T10, described initially on T-ALLcells and normal thymocyte subses (21), are not T lineage or maturation stage restrictedbut are Save | |
1986 | Mann, GJ;Fox, RM; | Deoxyadenosine triphosphate as a mediator of deoxyguanosine toxicity in cultured T lymphoblasts | The Journal of clinical investigation | 3490493 | Allcells were maintained at 37C in suspension culture in Hepes-buffered Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% fetal bovine serum, glutamine (2 Save | |
1986 | Slater, LM;Sweet, P;Stupecky, M;Gupta, S; | Cyclosporin A reverses vincristine and daunorubicin resistance in acute lymphatic leukemia in vitro | The Journal of clinical investigation | 3457021 | It has recently been reported that human ALLcells have a 10-fold greater concentration of calmodulin than normal peripheral blood lymphocytes and that CsA is a potentinhibitor of T Save | |
1986 | Cowley, GP;Smith, JA;Gusterson, BA; | Increased EGF receptors on human squamous carcinoma cell lines | British journal of cancer | 2420349 | The binding kinetics for allcells examined showed the typical pattern of a high and low affinity receptor with dissociation constants of 10-10M and 1-9M respectively. Recent Save | |
1986 | Stewart, MA;Warnock, M;Wheeler, A;Wilkie, N;Mullins, JI;Onions, DE;Neil, JC; | Nucleotide sequences of a feline leukemia virus subgroup A envelope gene and long terminal repeat and evidence for the recombinational origin of subgroup B viruses | Journal of virology | 3009890 | FEA cells (21) were infected in mass culture with various FeLV isolates and passaged for 3 weeks to allow virus spread to allcells. DNA was purified from these cell cultures for use in Save | |
1986 | DiFrancesco, D;Ferroni, A;Mazzanti, M;Tromba, C; | Properties of the hyperpolarizing-activated current (if) in cells isolated from the rabbit sino-atrial node | The Journal of physiology | 2432247 | The reason for this discrepancy may be the current run-down that occurs, although to a highly variable degree, in allcells. This feature of if is treated insome detail below. Here it may be Save | |
1986 | Hartman, BJ;Tomasz, A; | Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureus | Antimicrobial agents and chemotherapy | 3488015 | explanation of heterogeneity is that allcells in the heterogeneous culture carry the genetic marker(s) of methicillin resistance, but for some reason the phenotypic expression of Save | |
1986 | Wang, CY;Bushkin, Y;Pica, R;Lane, C;McGrath, H;Posnett, DN; | Stimulation and expansion of a human T-cell subpopulation by a monoclonal antibody to T-cell receptor molecule | Hybridoma | 2429909 | Both the aand (3-chains from normal PBL-T blasts resolved in the pi range of 5.5-6.5, whereas the a- and /3-chains from HPB-ALLcells had acidic and basic pis of 4.0 and 7.0-8.5, Save | |
1986 | Steiness, E; | Positive inotropic drugs--digitalis | Acta medica Scandinavica. Supplementum | 3017057 | Digitalis is known to inhibit the Na+K+-ATPase in allcells in nearly all species. It has been aqyed that this effect induces increase of the inuacellular sodium concentnfion within the Save | |
1985 | Lehrer, RI;Daher, K;Ganz, T;Selsted, ME; | Direct inactivation of viruses by MCP-1 and MCP-2, natural peptide antibiotics from rabbit leukocytes | Journal of virology | 2985808 | Allcells used in this manner were obtained from the American Type Culture Collection except for MDBK cells, which were a gift from Debi Nayak. The virus-infected cell monolayers Save | |
1985 | Morris, CS;Hitchcock, E; | Immunocytochemistry of folliculo-stellate cells of normal and neoplastic human pituitary gland | Journal of clinical pathology | 3889065 | folliculo-stellate cells stain for GFAP and S100, although not allcells stained for S100 are GFAP positive. Cocchia and Miani" showed S100 protein within the stellate cells of the pars Save | |
1985 | Neyses, L;Locher, R;Stimpel, M;Streuli, R;Vetter, W; | Stereospecific modulation of the calcium channel in human erythrocytes by cholesterol and its oxidized derivatives | The Biochemical journal | 2581557 | Furthermore, allcells were energized to the same extent by ATP (about 1.7mM). Since the anion channel may be influenced by cholesterol (Schubert & Boss, 1982), we checked Save | |
1985 | Hueston, WD; | Herd summaries of individual cow somatic cell counts and mastitis control research | Thesis | and th e re fo re abnormal; 2) allcells in milk were desquamated se c re to ry cells of epithelialo rig in , and th e re fo re a normal p art of la c to genes is ; and 3) the cells in normal milk Save | ||
1984 | Buick, RN;Pollak, MN; | Perspectives on clonogenic tumor cells, stem cells, and oncogenes | Cancer research | 6386145 | In prokaryotes, control of proliferation is unrelated to the process of differentiation, and allcells have proliferative potential. As we have shown, this is not the case in tissues of higher Save | |
1984 | Gaukroger, JM;Wilson, L; | Protection of cells from methotrexate toxicity by 7-hydroxymethotrexate | British journal of cancer | 6590087 | Cell growth survival studies have revealed that 7-OH methotrexate is two orders of magnitude less cytotoxic to human melanoma and human acute lymphoblastic leukaemia (ALL) cells Save | |
1984 | Gussander, E;Adams, A; | Electron microscopic evidence for replication of circular Epstein-Barr virus genomes in latently infected Raji cells | Journal of virology | 6092676 | Circular forms, which can be isolated either in the covalently closed, supercoiled structure or as a relaxed, open DNA circle (16), have subsequently been found in allcells with more Save | |
1984 | Epstein, LM;Forney, JD; | Mendelian and non-mendelian mutations affecting surface antigen expression in Paramecium tetraurelia | Molecular and cellular biology | 6092921 | A genetic and molecular analysis of this mutant suggests that although the A gene is present in the micronucleus of allcells, it is lost during the formation of the macronucleus in the Save | |
1984 | Yinon, U; | Myopia induction in animals following alteration of the visual input during development: a review | Current eye research | 6713963 | For example, asfar as has been determined, allcells of the salamander are capable of ded if fe rent iat ion with appropriate stimulation. Extensive in vitro experimentation on amphibian Save | |
1984 | Nelson, DO;Graham, CA; | Effects of development and captopril treatment on central angiotensin II--sensitive neurons | Clinical and experimental hypertension. Part A, Theory and practice | 6398145 | Nearly allcells examined, including neurons which were relatively insensitive to A11 or saralasin, were potently ex | |
1983 | Overath, P;Czichos, J;Stock, U;Nonnengaesser, C; | Repression of glycoprotein synthesis and release of surface coat during transformation of Trypanosoma brucei | The EMBO journal | 6688990 | Six hours after transfer of the cells to medium SDM-79, ie, at a time when VSG synthesis has already ceased, allcells retain the coat (compare sample at t= 6 h with freshly harvested Save | |
1983 | Rowin, KS;Tanowitz, HB;Wittner, M;Nguyen, HT;Nadal-Ginard, B; | Inhibition of muscle differentiation by trypanosoma cruzi | Proceedings of the National Academy of Sciences of the United States of America | 6413976 | Normal quantities of 13 and y-actin were present in allcells. (B) Rehybridization of the RNA blot shown in A with 32P-labeled DNA withnonmuscle-specific sequencescoding Save | |
1983 | Meo, T;Gramsch, C;Inan, R;Höllt, V;Weber, E;Herz, A;Riethmüller, G; | Monoclonal antibody to the message sequence Tyr-Gly-Gly-Phe of opioid peptides exhibits the specificity requirements of mammalian opioid receptors | Proceedings of the National Academy of Sciences of the United States of America | 6191329 | Allcells in the intermediate lobe of rat pituitary were intensely stained (Fig. 3). Higher magnification revealed heavy cytoplasmic localization of the immunoreactive peptides. Save | |
1983 | Otteskog, P;Sundqvist, KG; | Anchorage and lymphocyte function. Spreading-capacity distinguishes common thymocytes and peripheral T lymphocytes | Immunology | 6339376 | T-enriched human blood lymphocytes with an adhesive surface in the presence of Concanavalin A (Con A) almost immediately induced a sequence of motilechanges in virtually allcells Save | |
1983 | O'NEIL, PM; | Log-linear models for evaluating hunting demand | Thesis | Both the Pearson ch i-sq ua re statistic and th e lik e lih oodratio sum over allcells in th e ta b le . Both a re asymp to tic a lly d is tribu te d as chi - square . Save | ||
1983 | Burman, LG;Raichler, J;Park, JT; | Evidence for diffuse growth of the cylindrical portion of the Escherichia coli murein sacculus | Journal of bacteriology | 6350274 | The lengths of allcells that contained grains and the distances of the grains from the nearest cell pole were measured with a ruler. Forplotting, these distances were expressed as Save | |
2023 | Aashna Karbhari ,Sherly Mosessian ,Kamaxi H. Trivedi,Frank Valla Jr,Mark Jacobson,Mark J. Truty,Nandakumar G. Patnam,Diane M. Simeone,Elcin Zan,Tracy Brennan,Hongli Chen,Phillip H. Kuo,Ken Herrmann,Ajit H. Goenka | Gallium-68-labeled fibroblast activation protein inhibitor-46 PET in patients with resectable or borderline resectable pancreatic ductal adenocarcinoma: A phase 2, multicenter, single arm, open label non-randomized study protocol | PLOS One | Full Paper | ||
2023 | Cheong, JG;Ravishankar, A;Sharma, S;Parkhurst, CN;Grassmann, SA;Wingert, CK;Laurent, P;Ma, S;Paddock, L;Miranda, IC;Karakaslar, EO;Nehar-Belaid, D;Thibodeau, A;Bale, MJ;Kartha, VK;Yee, JK;Mays, MY;Jiang, C;Daman, AW;Martinez de Paz, A;Ahimovic, D;Ramos, V;Lercher, A;Nielsen, E;Alvarez-Mulett, S;Zheng, L;Earl, A;Yallowitz, A;Robbins, L;LaFond, E;Weidman, KL;Racine-Brzostek, S;Yang, HS;Price, DR;Leyre, L;Rendeiro, AF;Ravichandran, H;Kim, J;Borczuk, AC;Rice, CM;Jones, RB;Schenck, EJ;Kaner, RJ;Chadburn, A;Zhao, Z;Pascual, V;Elemento, O;Schwartz, RE;Buenrostro, JD;Niec, RE;Barrat, FJ;Lief, L;Sun, JC;Ucar, D;Josefowicz, SZ; | Epigenetic memory of coronavirus infection in innate immune cells and their progenitors | Cell | 37597510 | Paired PBMC and BMMC AllCells N/A Whole blood from study participants Weill-Cornell Medicine/New York-Presbyterian Hospital N/A | Full Paper |
2023 | Jhaveri, K;Eli, LD;Wildiers, H;Hurvitz, SA;Guerrero-Zotano, A;Unni, N;Brufsky, A;Park, H;Waisman, J;Yang, ES;Spanggaard, I;Reid, S;Burkard, ME;Vinayak, S;Prat, A;Arnedos, M;Bidard, FC;Loi, S;Crown, J;Bhave, M;Piha-Paul, SA;Suga, JM;Chia, S;Saura, C;Garcia-Saenz, JÁ;Gambardella, V;de Miguel, MJ;Gal-Yam, EN;Rapael, A;Stemmer, SM;Ma, C;Hanker, AB;Ye, D;Goldman, JW;Bose, R;Peterson, L;Bell, JSK;Frazier, A;DiPrimeo, D;Wong, A;Arteaga, CL;Solit, DB; | Neratinib + fulvestrant + trastuzumab for HR-positive, HER2-negative, HER2-mutant metastatic breast cancer: outcomes and biomarker analysis from the SUMMIT trial | Annals of oncology : official journal of the European Society for Medical Oncology | 37597578 | Background HER2 mutations are targetable alterations in patients with hormone-receptor positive (HR+) metastatic breast cancer (MBC). In the SUMMIT basket study, Save | Full Paper |
2023 | Fiumara, M;Ferrari, S;Omer-Javed, A;Beretta, S;Albano, L;Canarutto, D;Varesi, A;Gaddoni, C;Brombin, C;Cugnata, F;Zonari, E;Naldini, MM;Barcella, M;Gentner, B;Merelli, I;Naldini, L; | Genotoxic effects of base and prime editing in human hematopoietic stem cells | Nature biotechnology | 37679541 | Granulocyte colony-stimulating factor (G-CSF) or G-CSF + plerixafor mPB CD34+ HSPCs were purified in-house with the CliniMACS CD34 Reagent system (Miltenyi Biotec) from Mobilized Leukopak (AllCells) according to the TIGET-HPCT protocol approved by the OSR Ethical Committee and following the manufacturer’s instructions. HSPCs were seede | Full Paper |
2023 | De Rop, FV;Hulselmans, G;Flerin, C;Soler-Vila, P;Rafels, A;Christiaens, V;González-Blas, CB;Marchese, D;Caratù, G;Poovathingal, S;Rozenblatt-Rosen, O;Slyper, M;Luo, W;Muus, C;Duarte, F;Shrestha, R;Bagdatli, ST;Corces, MR;Mamanova, L;Knights, A;Meyer, KB;Mulqueen, R;Taherinasab, A;Maschmeyer, P;Pezoldt, J;Lambert, CLG;Iglesias, M;Najle, SR;Dossani, ZY;Martelotto, LG;Burkett, Z;Lebofsky, R;Martin-Subero, JI;Pillai, S;Sebé-Pedrós, A;Deplancke, B;Teichmann, SA;Ludwig, LS;Braun, TP;Adey, AC;Greenleaf, WJ;Buenrostro, JD;Regev, A;Aerts, S;Heyn, H; | Systematic benchmarking of single-cell ATAC-sequencing protocols | Nature biotechnology | 37537502 | Cryopreserved human PBMCs from one male donor and one female donor were purchased from AllCells and distributed across institutes to generate the following Save | Full Paper |
2023 | Cascone, T;Kar, G;Spicer, JD;Garcia-Campelo, R;Weder, W;Daniel, DB;Spigel, DR;Hussein, M;Mazieres, J;Oliveira, J;Yau, EH;Spira, AI;Anagnostou, V;Mager, R;Hamid, O;Cheng, LY;Zheng, Y;Blando, J;Tan, TH;Surace, M;Rodriguez-Canales, J;Gopalakrishnan, V;Sellman, BR;Grenga, I;Soo-Hoo, Y;Kumar, R;McGrath, L;Forde, PM; | Neoadjuvant Durvalumab Alone or Combined with Novel Immuno-Oncology Agents in Resectable Lung Cancer: The Phase 2 NeoCOAST Platform Trial | Cancer discovery | 37707791 | Messenger RNA was extracted from pre-treatment and on-treatment FFPE tumor tissue, when available, using the HudsonAlpha Discovery FFPE Tissue Extraction Method Save | Full Paper |
2023 | Saura, C;Ortiz, C;Matito, J;Arenas, EJ;Suñol, A;Martín, Á;Córdoba, O;Martínez-Sabadell, A;García-Ruiz, I;Miranda, I;Morales-Comas, C;Carrasco, E;Viaplana, C;Peg, V;Nuciforo, P;Bayó-Puxan, N;Gonzalez-Medina, A;Miquel, JM;Gómez-Rey, M;Villacampa, G;Arévalo, S;Espinosa-Bravo, M;Balmaña, J;Dienstmann, R;Arribas, J;Tabernero, J;Vivancos, A;Sansó, M; | Early-Stage Breast Cancer Detection in Breast Milk | Cancer discovery | 37704212 | f the study. V. Peg reports personal fees from Roche, AstraZeneca, Sysmex, and Merck Sharp & Dohme outside the submitted work. P. Nuciforo reports personal fees from Bayer, MSD Oncology, Novartis, and Targos Molecular Pathology GmbH outside the submitted work. M. Gómez-Rey reports nonfinancial support from the Cellex Foundation during the conduct of the study. G. Villacampa reports perso | Full Paper |
2023 | Reggiardo, RE;Maroli, SV;Peddu, V;Davidson, AE;Hill, A;LaMontagne, E;Aaraj, YA;Jain, M;Chan, SY;Kim, DH; | Profiling of repetitive RNA sequences in the blood plasma of patients with cancer | Nature biomedical engineering | 37652985 | ExoRNeasy kit (Qiagen) was used to isolate cell-free RNA from blood plasma of de-identified healthy controls (blood collected in K2EDTA tubes; Discovery Life Sciences) Save | Full Paper |
2023 | Li, Y;Xue, M;Deng, X;Dong, L;Nguyen, LXT;Ren, L;Han, L;Li, C;Xue, J;Zhao, Z;Li, W;Qing, Y;Shen, C;Tan, B;Chen, Z;Leung, K;Wang, K;Swaminathan, S;Li, L;Wunderlich, M;Mulloy, JC;Li, X;Chen, H;Zhang, B;Horne, D;Rosen, ST;Marcucci, G;Xu, M;Li, Z;Wei, M;Tian, J;Shen, B;Su, R;Chen, J; | TET2-mediated mRNA demethylation regulates leukemia stem cell homing and self-renewal | Cell stem cell | 37541212 | TET2 is recurrently mutated in acute myeloid leukemia (AML) and its deficiency promotes leukemogenesis (driven by aggressive oncogenic mutations) and enhances Save;Recombinant Mouse IFN-b R&D SYSTEMS Cat# 8234-MB-010 ColonyGEL-Mouse Base Medium Reachbio Cat# 1201 FastDigest NotI Thermo Fisher Scientific Cat# FD0595 | Full Paper |
2023 | Juric, V;Mayes, E;Binnewies, M;Lee, T;Canaday, P;Pollack, JL;Rudolph, J;Du, X;Liu, VM;Dash, S;Palmer, R;Jahchan, NS;Ramoth, ÅJ;Lacayo, S;Mankikar, S;Norng, M;Brassell, C;Pal, A;Chan, C;Lu, E;Sriram, V;Streuli, M;Krummel, MF;Baker, KP;Liang, L; | TREM1 activation of myeloid cells promotes antitumor immunity | Science translational medicine | 37647386 | Dissociated human tumor cells (Discovery Life Sciences) were stained with Zombie NIR viability dye, FcR-blocked with Human TrueStain FcX (BioLegend), and stained Save | Full Paper |
2023 | Hong, D;Jo, EJ;Bang, D;Jung, C;Lee, YE;Noh, YS;Shin, MG;Kim, MG; | Plasmonic Approach to Fluorescence Enhancement of Mesoporous Silica-Coated Gold Nanorods for Highly Sensitive Influenza A Virus Detection Using Lateral Flow Immunosensor | ACS nano | 37595106 | IAV and IBV patient samples for discovery cohort analysis were purchased from Discovery Life Sciences (Los Osos, CA). IAV patient samples for validation cohort analysis Save | Full Paper |
2023 | Worboys, JD;Vowell, KN;Hare, RK;Ambrose, AR;Bertuzzi, M;Conner, MA;Patel, FP;Zammit, WH;Gali-Moya, J;Hazime, KS;Jones, KL;Rey, C;Jonjic, S;Rovis, TL;Tannahill, GM;Cruz De Matos, GDS;Waight, JD;Davis, DM; | TIGIT can inhibit T cell activation via ligation-induced nanoclusters, independent of CD226 co-stimulation | Nature communications | 37596248 | Tumour samples and associated reference peripheral blood samples were acquired from the commercial vendor Discovery Life Sciences under Task Order DISLS06 as Save;f Manchester, UK) as approved by the West Midlands - Black Country Research Ethics Committee. Tumour samples and associated reference peripheral blood samples were acquired from the commercial vendor Discovery Life Sciences under Task Order DISLS06 as approved by GSK HBSM Due Diligence. Cell lines The Jurkat cell line was obtained from the ATCC (Clone E6-1; ATCC TIB-152 ; RRID:CVCL_0367) and | Full Paper |
2023 | Santos, MF;Rappa, G;Karbanová, J;Diana, P;Cirrincione, G;Carbone, D;Manna, D;Aalam, F;Wang, D;Vanier, C;Corbeil, D;Lorico, A; | HIV-1-induced nuclear invaginations mediated by VAP-A, ORP3, and Rab7 complex explain infection of activated T cells | Nature communications | 37563144 | one 1022) were acquired from NIH HIV Reagent Program, Division of AIDS, NIAID, NIH104. They were cultured in DMEM (#11995065, Thermo Fisher Scientific). Human helper T (CD4+) cells were obtained from Discovery Life Sciences (#C4T0015-Z1110032763081618BA). We used five distinct lots of CD4+ T cells. As stated by the vendor, these primary cells were obtained from a Caucasian male (patient ID:1100327 | Full Paper |
2023 | Bekerman, E;Yant, SR;VanderVeen, L;Hansen, D;Lu, B;Rowe, W;Wang, K;Callebaut, C; | Long-acting lenacapavir acts as an effective preexposure prophylaxis in a rectal SHIV challenge macaque model | The Journal of clinical investigation | 37384413 | Human PBMCs were collected from healthy volunteers after informed consent, and their use was approved by an institutional review board at AllCells (Alameda, California Save | Full Paper |
2023 | Lin, W;Zhang, Y;Yang, Y;Lin, B;Zhu, M;Xu, J;Chen, Y;Wu, W;Chen, B;Chen, X;Liu, J;Wang, H;Teng, F;Yu, X;Wang, H;Lu, J;Zhou, Q;Teng, L; | Anti-PD-1/Her2 Bispecific Antibody IBI315 Enhances the Treatment Effect of Her2-Positive Gastric Cancer through Gasdermin B-Cleavage Induced Pyroptosis | Advanced science (Weinheim, Baden-Wurttemberg, Germany) | 37587833 | lone) supplemented with 10% FBS. Human PBMCs (P121080605C and LP220727010) were obtained from ALLCELLS (Shanghai, China) and cultured in RPMI medium with 10% heat-inactivated FBS | Full Paper |
2023 | Marone, R;Landmann, E;Devaux, A;Lepore, R;Seyres, D;Zuin, J;Burgold, T;Engdahl, C;Capoferri, G;Dell'Aglio, A;Larrue, C;Simonetta, F;Rositzka, J;Rhiel, M;Andrieux, G;Gallagher, DN;Schröder, MS;Wiederkehr, A;Sinopoli, A;Do Sacramento, V;Haydn, A;Garcia-Prat, L;Divsalar, C;Camus, A;Xu, L;Bordoli, L;Schwede, T;Porteus, M;Tamburini, J;Corn, JE;Cathomen, T;Cornu, TI;Urlinger, S;Jeker, LT; | Epitope-engineered human hematopoietic stem cells are shielded from CD123-targeted immunotherapy | The Journal of experimental medicine | 37773046 | and the plate was incubated at 37°C. CD123 and CCR5 gene editing with CRISPR/Cas9 and rAAV6 virus CD34+ HSPC culture condition Plerixafor-mobilized peripheral blood CD34+ HSPCs were purchased from AllCells. The cells were thawed as per the manufacturer’s instructions and cultured at 37°C, 5% CO2, and 5% O2. Cell culture medium was GMP stem cell growth medium (SCGM) medium from CellGenix supp | Full Paper |
2023 | Chen, C;Shen, M;Wan, X;Sheng, L;He, Y;Xu, M;Yuan, M;Ji, Z;Zhang, J; | Activated T cell-derived exosomes for targeted delivery of AXL-siRNA loaded paclitaxel-poly-L-lysine prodrug to overcome drug resistance in triple-negative breast cancer | Chemical Engineering Journal | Academy of Sciences (Shanghai, China). Human PBMCs (hPBMCs) were purchased from AllCells Technologies (Alameda, USA). All experiments conducted with human samples were approv | Full Paper | |
2023 | Reyes-Castro, RA;Chen, SY;Seemann, J;Kundu, ST;Gibbons, DL;Arur, S; | Phosphorylated nuclear DICER1 promotes open chromatin state and lineage plasticity of AT2 tumor cells in lung adenocarcinomas | Science advances | 37494452 | Tumor microarrays (TMAs) with de-identified patient information were obtained from Discovery Life Sciences (Powell, OH) and TriStar Technology Group LLC (Washington Save;oth genders were used. No power analysis was used to determine the sample size. Human LUAD tumor tissue microarrays Tumor microarrays (TMAs) with de-identified patient information were obtained from Discovery Life Sciences (Powell, OH) and TriStar Technology Group LLC (Washington, DC). Eighty-eight LUAD samples from untreated patients were used. No patient or human subjects were recruited for thi | Full Paper |
2023 | Fischer, M;Gerrick, K;Belmontes, B;Slemmons, K;Freyman, Y;Jain, J;Federowicz, S;Bishof, I;Rao, A;Fleury, M;Mounir, Z;Lackner, M;Hughes, P;White, M;Neilan, C; | Abstract 1644: Dual inhibition of MAT2A and PRMT5 delivers synergistic anti-tumor responses in preclinical models of MTAP-deleted cancer | Cancer Research | Pharmaron, Charles River, Wuxi Apptec, Champions Oncology, Xentech, and Crown Biosciences for performing in vivo studies 2. HudsonAlpha Discovery for RNA sequencing 3. Fios Genomics and Directed Bio for gene expression and splicing analysis 4. Mosaic Laboratories for SDMA IHC | Full Paper | |
2023 | Gerrick, K;Laraio, J;Annen, K;Tran, H;Fischer, M;Freyman, Y;Sharma, G;Bishof, I;Federowicz, S;Pankajakshan, D;Neilan, C;Mangatt, B;Mazurek, A;Lackner, M;White, M;Mounir, Z; | Abstract 1637: MAT2A inhibition in MTAP-/- tumors confers mechanistic vulnerabilities to multiple clinically actionable synthetic lethal drug combinations | Cancer Research | able contributions from Dr. Christian Frey, Dr. Jeanne Quirit, Dr. Neil Bhola, Atieh Givmanesh, and John Faulhaber. We are grateful to our CRO partners Horizon Discovery, Eurofins, DepMap Consortium, and HudsonAlpha Discovery | Full Paper | |
2023 | Kumar, A;Estes Bright, LM;Garren, MRS;Manuel, J;Shome, A;Handa, H; | Chemical Modification of Tiopronin for Dual Management of Cystinuria and Associated Bacterial Infections | ACS applied materials & interfaces | 37671841 | The urine sample was obtained from Discovery Life Sciences (Huntsville, AL) and work was performed according to the Use of Laboratory Sample Guidelines by the Save;b holders, sputter-coated with gold-palladium, and observed with an FEI Teneo Scanning Electron Microscope (Thermo Fisher, Waltham, MA). Urine Sample Preparation The urine sample was obtained from Discovery Life Sciences (Huntsville, AL) and work was performed according to the Use of Laboratory Sample Guidelines by the University of Georgia to ensure appropriate handling and disposal of clinica | Full Paper |
2023 | Kannan, K;Kumar, K;Dhivagar, R;Sathyanarayanan, S;Parameshwaran, R;Singh, P; | An experimental study on heat power stored by thermal dissipation in electronic components using thermosyphon integrated hybrid nanocomposite phase change material | Journal of Energy Storage | The melting ranges of the three PCMs used were 42 °C, 55 °C and 58 °C for PureTemp, allcells PCC, and pure paraffin wax, respectively. The operating time of electronic equipment is reportedly increased as a result of PCM's ability to stabilise the device temperat | Full Paper | |
2023 | Macaulay, VM;Lord, S;Hussain, S;Maroto, JP;Jones, RH;Climent, MÁ;Cook, N;Lin, CC;Wang, SS;Bianchini, D;Bailey, M;Schlieker, L;Bogenrieder, T;de Bono, J; | A Phase Ib/II study of IGF-neutralising antibody xentuzumab with enzalutamide in metastatic castration-resistant prostate cancer | British journal of cancer | 37537253 | assessed by immunohistochemistry (IHC) in archival formalin-fixed, paraffin-embedded (FFPE) tumour samples and was undertaken centrally by Discovery Life Sciences ( Save;lation in cells expressing human IGF-1R. PTEN expression was assessed by immunohistochemistry (IHC) in archival formalin-fixed, paraffin-embedded (FFPE) tumour samples and was undertaken centrally by Discovery Life Sciences (Kassel, Germany) using the antibody clone 138G6 (cat #9559, Cell Signaling Technology) and the fully automated Ventana Benchmark Ultra platform following the Ultra View DAB pr | Full Paper |
2023 | Sheng, XR;Gao, X;Schiffman, C;Jiang, J;Ramalingam, TR;Lin, CJF;Khanna, D;Neighbors, M; | Biomarkers of fibrosis, inflammation, and extracellular matrix in the phase 3 trial of tocilizumab in systemic sclerosis | Clinical immunology (Orlando, Fla.) | 37479123 | A separate set of HC serum samples (HC2s; collected by Discovery Life Sciences) was Discovery Life Sciences and BioIVT provided ethically collected healthy donor Save;ained from the Genentech Samples for Science program (South San Francisco, CA), Discovery Life Sciences (Huntsville, AL), and BioIVT (Hicksville, NY) was processed and stored according to standard sampling procedures, and assayed for CCL18, YKL-40, CXCL13, COMP, | Full Paper |
2023 | Pujals, M;Mayans, C;Bellio, C;Méndez, O;Greco, E;Fasani, R;Alemany-Chavarria, M;Zamora, E;Padilla, L;Mitjans, F;Nuciforo, P;Canals, F;Nonell, L;Abad, M;Saura, C;Tabernero, J;Villanueva, J; | RAGE/SNAIL1 signaling drives epithelial-mesenchymal plasticity in metastatic triple-negative breast cancer | Oncogene | 37468678 | Epithelial/Mesenchymal (E/M) plasticity plays a fundamental role both in embryogenesis and during tumorigenesis. The receptor for advanced glycation end products ( Save | Full Paper |
2023 | Birker, K;Ge, S;Kirkland, NJ;Theis, JL;Marchant, J;Fogarty, ZC;Missinato, MA;Kalvakuri, S;Grossfeld, P;Engler, AJ;Ocorr, K;Nelson, TJ;Colas, AR;Olson, TM;Vogler, G;Bodmer, R; | Mitochondrial MICOS complex genes, implicated in hypoplastic left heart syndrome, maintain cardiac contractility and actomyosin integrity | eLife | 37404133 | WGS was performed on samples from 183 individuals with HLHS and 496 family members by the Mayo Clinic Medical Genome Facility or Discovery Life Sciences. SNVs and INDELs that passed quality control were subject to further filtering based upon rarity (MAF 0.01) and predicted consequence. Details about the sequencing and subsequent variant filteri | Full Paper |
2023 | Khan, DMIO;Karmaus, PWF;Bach, A;Crawford, RB;Kaminski, NE; | An in vitro model of human hematopoiesis identifies a regulatory role for the aryl hydrocarbon receptor | Blood advances | 37477592 | In vitro models to study simultaneous development of different human immune cells and hematopoietic lineages are lacking. We identified and characterized, using single- Save;Human CD34+ 91 HSPCs isolated from umbilical cord blood from mixed donors were purchased in cryopreserved state from AllCells LLC (CA, USA). Approxi | Full Paper |
2023 | Klein, S;Schulte, A;Arolt, C;Tolkach, Y;Reinhardt, HC;Buettner, R;Quaas, A; | Intratumoral Abundance of M2-Macrophages is Associated With Unfavorable Prognosis and Markers of T-Cell Exhaustion in Small Cell Lung Cancer Patients | Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc | 37423586 | RB is co-founder and scientific advisor for Targos Molecular Pathology Inc and member of the Directory Board Gnothis Inc. HCR received consulting and lecture fees from Save | Full Paper |
2023 | Jia, Z;Li, Z;Liu, C; | CRISPR-powered Biosensing Platform for Quantitative Detection of Alpha-fetoprotein by a Personal Glucose Meter | Sensors and actuators. B, Chemical | 37303825 | Serum samples from patients with liver cancer were obtained from Discovery Life Sciences. Human AFP ELISA kits were purchased from RayBiotech, Inc. PGMs (Accu- Save;ns, Inc. Serum samples from healthy individuals were obtained from Innovative Research, Inc. Serum samples from patients with liver cancer were obtained from Discovery Life Sciences. Human AFP ELISA kits were purchased from RayBiotech, Inc. PGMs (Accu-Chek Aviva Plus) and test strips were purchased from Roche Diagnostics Co., Ltd. All chemica | Full Paper |
2023 | Leatham, B;McNall, K;Subramanian, HKK;Jacky, L;Alvarado, J;Yurk, D;Wang, M;Green, DC;Tsongalis, GJ;Rajagopal, A;Schwartz, JJ; | A rapid, multiplex digital PCR assay to detect gene variants and fusions in non-small cell lung cancer | Molecular oncology | 37714814 | A second set of de-identified, remnant FFPE samples greater than 15 years old were sourced from Discovery Life Sciences (includes samples through acquisitions of Save;- Digital PCR (dPCR) is emerging as an ideal platform for the detection and tracking of genomic variants in cancer due to its high sensitivity and simple workflow. The Save | Full Paper |
2023 | Sullivan, AT;Rao, V;Rockwood, T;Gandhi, J;Gruzka, S;O'Connor, L;Wang, B;Ragan, KB;Zhang, DY;Khodakov, D; | Rapid, tunable, and multiplexed detection of RNA using convective array PCR | Communications biology | 37741867 | VTM samples testing negative for presence of SARS-CoV-2 were purchased from Discovery Life Sciences' sample repository. Sample lysis and nucleic acid purification Save | Full Paper |
2023 | Salek, M;Li, N;Chou, HP;Saini, K;Jovic, A;Jacobs, KB;Johnson, C;Lu, V;Lee, EJ;Chang, C;Nguyen, P;Mei, J;Pant, KP;Wong-Thai, AY;Smith, QF;Huang, S;Chow, R;Cruz, J;Walker, J;Chan, B;Musci, TJ;Ashley, EA;Masaeli, MM; | COSMOS: a platform for real-time morphology-based, label-free cell sorting using deep learning | Communications biology | 37740030 | lls and cancer cells before and after CD45 depletion. The remaining sample was processed, and A549 cells were sorted on COSMOS. Dissociated tumor cells (DTCs) from NSCLC patients were purchased from Discovery Life Sciences (DLS; Los Osos, CA, USA). Cancer type, stage information, and cell type composition reports from flow cytometry were provided by the vendor. To account for possible cell type c | Full Paper |
2023 | Gail, D;Suzart, V;Du, W;Kaur Sandhu, A;Jarvela, J;Nantongo, M;Mwebaza, I;Panigrahi, S;Freeman, M;Canaday, D;Boom, W;Silver, R;Carpenter, S; | Mycobacterium tuberculosis impairs human memory CD4+ T cell recognition of M2 but not M1-like macrophages | iScience | ood from healthy volunteers with latent Mtb infection University Hospitals IRB protocol N/A PBMCs from leukapheresis product collected from healthy, CMV seropositive volunteers Purchased from AllCells, Inc. N/A Bronchoalveolar Lavage Samples isolated from healthy volunteers Obtained by Dr. Richard Silver, CWRU School of Medicine Ref. 92 ------------------------- Chemicals, pepti;PBMCs from leukapheresis product collected from healthy, CMV seropositive volunteers Purchased from AllCells, Inc. N/A | Full Paper | |
2023 | Faccioli, LAP;Cetin, Z;Kocas-Kilicarslan, ZN;Ortiz, K;Sun, Y;Hu, Z;Kurihara, T;Tafaleng, EN;Florentino, RM;Wang, Z;Xia, M;Miedel, MT;Taylor, DL;Behari, J;Ostrowska, A;Constantine, R;Li, A;Soto-Gutierrez, A; | Evaluation of Human Hepatocyte Drug Metabolism Carrying High-Risk or Protection-Associated Liver Disease Genetic Variants | International journal of molecular sciences | 37686209 | Metabolic-dysfunction-associated steatotic liver disease (MASLD), which affects 30 million people in the US and is anticipated to reach over 100 million by 2030, places a Save Cached;(CYP) enzymes, polymorphic sites associated with high-risk liver disease progression and protection were determined in 126 human hepatocytes. The cryopreserved primary hepatocytes were obtained from In Vitro ADMET Laboratories Inc. (IVAL, Columbia, MD, USA). The isolations were procured from human liver donors that were rejected for orthotopic liver transplantation. Liver tissue was equally distr;Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.||Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, PA 15261, USA.++Department of Statistics, University of Pittsburgh, Pittsburgh, PA 15213, USA.++Drug Discovery Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.||Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA.++Drug Discovery Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.||Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA.++Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, PA 15261, USA.||Department of Statistics, University of Pittsburgh, Pittsburgh, PA 15213, USA.||Drug Discovery Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.++Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, PA 15261, USA.||Department of Medicine, Division of Gastroenterology, Hepatology, and Nutrition, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.||Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, PA 15261, USA.++Discovery Life Sciences, Huntsville, AL 35806, USA.++Discovery Life Sciences, Huntsville, AL 35806, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.||Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, PA 15261, USA.||Drug Discovery Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.||McGowan Institute for Regenerative Medicine, Pittsburgh, PA 15219, USA | Full Paper |
2023 | Wisdom, KM;Suijker, J;Van den Broek, L;Sridharan, B;Grandhi, TSP;Cheng, A;Lamb, M;Titus, SA;Poore, D;Shah, N;Cheng, SH;Kim, E;Griffin, S;Ekert, J; | Lung tumor microphysiological system with 3D endothelium to evaluate modulators of T-cell migration | ALTEX | 37422924 | Primary human T-Cells (AllCells, StemExpress, or BioIVT) were thawed in one of the , T-cells were obtained directly from AllCells and shipped to the MIMETAS research Save | Full Paper |
2023 | Chiang, YP;Li, Z;He, M;Jones, Q;Pan, M;Han, X;Jiang, XC; | Sphingomyelin synthase-related protein SMSr is a phosphatidylethanolamine phospholipase C that promotes nonalcoholic fatty liver disease | The Journal of biological chemistry | 37586586 | PE-PLC and PE levels in NASH patients. Human liver microarrays with normal and NASH sections were purchased from Sekisui XenoTech, LLC. Human female NASH plasma and control plasma were purchased from Discovery Life Sciences. A and B, SMSr immunostaining was performed on liver sections from human NASH patients and controls. Quantification was shown by fluorogram. C and D, NASH patient and control p | Full Paper |
2023 | Rong, Y;Torres-Luna, C;Tuszynski, G;Siderits, R;Chang, FN; | Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1) | Cancers | 37444514 | In brief, tissue staining was performed by Discovery Life Sciences (Newtown, PA, USA) according to standard procedures using optimized antibody concentration and Save Cached | Full Paper |
2023 | Lam, GT;Sorvina, A;Martini, C;Prabhakaran, S;Ung, BS;Lazniewska, J;Moore, CR;Beck, AR;Hopkins, AM;Johnson, IRD;Caruso, MC;Hickey, SM;Brooks, RD;Jackett, L;Karageorgos, L;Foster-Smith, EJ;Malone, V;Klebe, S;O'Leary, JJ;Brooks, DA;Logan, JM; | Altered endosomal-lysosomal biogenesis in melanoma | Neoplasia (New York, N.Y.) | 37562257 | ssue samples, including three normal skin samples and 23 biopsies from patients with melanoma (14 of which have normal adjacent regions), which were obtained from BioIVT (Michigan, United States) and Discovery Life Sciences (California, United States), with legal informed consent and relevant clinical information. Tumor stage was classified according to the American Joint Committee on Cancer and U | Full Paper |
2023 | Arakawa, M;Sakamoto, Y;Miyagawa, Y;Nito, C;Takahashi, S;Nitahara-Kasahara, Y;Suda, S;Yamazaki, Y;Sakai, M;Kimura, K;Okada, T; | iPSC-derived mesenchymal stem cells attenuate cerebral ischemia-reperfusion injury by inhibiting inflammatory signaling and oxidative stress | Molecular therapy. Methods & clinical development | 37637385 | lates coated with Matrigel (Corning, Corning, NY, USA). The BMMSCs were provided by Kaneka (Tokyo, Japan), and the manufacturing process of BMMSCs was as follows. Human bone marrow was purchased from AllCells (Emeryville, CA, USA); 50 mL of bone marrow was diluted 4-fold with minimum essential medium α (MEM α) (Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS | Full Paper |
2023 | Xu, L;Lahiri, P;Skowronski, J;Bhatia, N;Lattanzi, A;Porteus, MH; | Molecular dynamics of genome editing with CRISPR-Cas9 and rAAV6 virus in human HSPCs to treat sickle cell disease | Molecular therapy. Methods & clinical development | 37637384 | cultured as previously described.5,20 Human CB-CD34+ HSPCs were generously provided by the Binns Program for Cord Blood Research at Stanford University. Plerixafor mPB-CD34+ HSPCs were purchased from AllCells (Alameda, CA, USA) or received from the Stanford LCGM Facility. The cells were thawed per the manufacturer’s instructions and were cultured at 37°C, 5% CO2, and 5% O2 for 48 h (48-h pre-s | Full Paper |
2023 | Murray, J;Einhaus, T;Venkataraman, R;Radtke, S;Zhen, A;Carrillo, MA;Kitchen, SG;Peterson, CW;Kiem, HP; | Efficient manufacturing and engraftment of CCR5 gene-edited HSPCs following busulfan conditioning in nonhuman primates | Molecular therapy. Methods & clinical development | 37575091 | ng Container (Thermo Fisher Scientific, Waltham, MA) and stored at −80° for up to 2 weeks until infusion. CFC assays A total 1,000 to 1,200 enriched cells were seeded into 3.5-mL ColonyGEL 1402 (ReachBio, Seattle, WA) in 35-mm petri dishes. Hematopoietic colonies were scored after 12 to 14 days. Arising colonies were identified as colony-forming unit (CFU) granulocyte (CFU-G), CFU macrophage | Full Paper |
2023 | Petty, N;Radtke, S;Fields, E;Humbert, O;Llewlyn, M;Laszlo, G;Zhu, H;Jerome, K;Walter, R;Kiem, H; | Efficient Long-Term Multilineage Engraftment of CD33-Edited Hematopoietic Stem/Progenitor Cells in Nonhuman Primates | Molecular Therapy - Methods & Clinical Development | Current immunotherapeutic targets are often shared between neoplastic and normal hematopoietic stem/progenitor cells (HSPCs), leading to unwanted on-target, off- Save;For CFC assays, 1000 to 1200 sorted cells were seeded into 3.5-mL ColonyGEL 1402 (ReachBio, 305 Seattle, WA). Hematopoietic colonies were scored after 12 to 14 days. Arising colonies were 306 ident | Full Paper | |
2023 | Boccacci, Y;Dumont, N;Doyon, Y;Laganière, J; | Accessory-cell-free differentiation of hematopoietic stem and progenitor cells into mature red blood cells | Cytotherapy | 37598334 | Full Paper | |
2023 | Rathore, AS;Gupta, KK;Govindaraj, SK;Ajmani, A;Arivalagan, J;Anto, RJ;Kalishwaralal, K;Chandran, SA; | Targeting BRF2: insights from in silico screening and molecular dynamic simulations | Journal of biomolecular structure & dynamics | 37705251 | School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, India.++School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, India.++School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, India.++Division of Cancer Research, Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, India.++Discovery Life Sciences, Malden, MA, USA.++Division of Cancer Research, Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, India.++Division of Cancer Research, Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, India.++School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, India | Full Paper |
2023 | Mwebaza, I;Shaw, R;Li, Q;Fletcher, S;Achkar, JM;Harding, CV;Carpenter, SM;Boom, WH; | Impact of Mycobacterium tuberculosis Glycolipids on the CD4+ T Cell-Macrophage Immunological Synapse | Journal of immunology (Baltimore, Md. : 1950) | 37695687 | + T cells and monocytes were also obtained from three adult donors by leukapheresis from AllCells (Almeda, CA). In both cases, all samples were deidentified, and the Save | Full Paper |
2023 | Gao, J;Ren, S;Choonoo, G;Chen, G;Frleta, D;Zhong, J;Gupta, N;Sharma, P;Oyejide, A;Atwal, GS;Macdonald, L;Murphy, A;Kuhnert, F; | Microenvironment-dependent growth of Sezary cells in humanized IL-15 mice | Disease models & mechanisms | 37718909 | Table 1) or healthy control individuals were purchased from Discovery Life Sciences. 5x105 to 1x106 thawed total PBMC cells were intravenously transplanted into sub- Save | Full Paper |
2023 | Alluhayb, AH;Severance, C;Hendry-Hofer, T;Bebarta, VS;Logue, BA; | Concurrent determination of cyanide and thiocyanate in human and swine antemortem and postmortem blood by high-performance liquid chromatography-tandem mass spectrometry | Analytical and bioanalytical chemistry | 37712953 | The blood was collected by Discovery Life Sciences (Huntsville, Alabama, USA) and shipped on dry ice to the Department of Chemistry and Biochemistry at South Dakota Save | Full Paper |
2023 | Zhuo, X;Howell, BA;Shen, H;Woodhead, JL;Mosure, K;Zhang, Y;Scialis, RJ;Iyer, R;Sun, Y;Boy, KM;Lentz, KA;Denton, RR;Soars, MG;Johnson, BM;Humphreys, WG; | Mechanistic investigation of liver injury induced by BMS-932481, an experimental ɣ-secretase modulator | Toxicological sciences : an official journal of the Society of Toxicology | 37261863 | Primary cryoplateable human hepatocytes (In Vitro ADMET Laboratories, Malden, Massachusetts) were plated on Seahorse 96-well plates (Agilent, Santa Clara, California Save | Full Paper |
2023 | Lam, J;Yu, J;Lee, B;Campagna, C;Yoo, S;Baek, K;Jeon, NL;Sung, KE; | Characterizing On-Chip Angiogenesis Induction in a Microphysiological System as a Functional Measure of Mesenchymal Stromal Cell Bioactivity | Advanced biology | 37409400 | B16; Frederick, MD) at passage 1 (P1), Lonza (8F560, 167 696; Walkersville, MD) at passage 2 (P2), and AllCells (PCBM1632, PCBM1641; Alameda, CA) at P2 (see Figure 3 for donor specifications). MS | Full Paper |
2023 | Morsczeck, C;Pieles, O;Beck, HC; | Analysis of the phosphoproteome in human dental follicle cells during osteogenic differentiation | European journal of oral sciences | 37664892 | Human DFCs were obtained commercially (AllCells). The standard culture medium was Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich) and 100 μg mL | Full Paper |
2023 | Rüschoff, J;Schildhaus, HU;Rüschoff, JH;Jöhrens, K;Bocker-Edmonston, T;Dietmaier, W;Bläker, H;Baretton, G;Horst, D;Dietel, M;Hartmann, A;Klauschen, F;Merkelbach-Bruse, S;Stenzinger, A;Schöniger, S;Tiemann, M;Weichert, W;Büttner, R; | [Testing deficient mismatch repair and microsatellite instability : A focused update. German version] | Pathologie (Heidelberg, Germany) | 37548948 | Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland. josef.rueschoff@dls.com.++Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland.++Institut für Pathologie und Molekularpathologie, Universitätsspital Zürich, Zürich, Schweiz.++Institut für Pathologie, Universitätsklinikum Carl Gustav Carus Dresden, Dresden, Deutschland.++Department of Pathology, Cooper University Health Care, Camden, NJ, USA.++Institut für Pathologie/Zentrum für molekularpathologische Diagnostik, Universität Regensburg, Regensburg, Deutschland.++Institut für Pathologie, Universitätsklinikum Leipzig, Leipzig, Deutschland.++Institut für Pathologie, Universitätsklinikum Carl Gustav Carus Dresden, Dresden, Deutschland.++Institut für Pathologie, Charité - Universitätsmedizin Berlin, Campus Mitte, Berlin, Deutschland.++Institut für Pathologie, Charité - Universitätsmedizin Berlin, Campus Mitte, Berlin, Deutschland.++Pathologisches Institut, Universität Erlangen-Nürnberg, Erlangen, Deutschland.++Pathologisches Institut, Ludwig-Maximilians-Universität München, München, Deutschland.++Institut für Pathologie, Universitätsklinikum Köln, Köln, Deutschland.++Pathologisches Institut, Universitätsklinikum Heidelberg, Heidelberg, Deutschland.++Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland.++Institut für Hämatopathologie Hamburg, Hamburg, Deutschland.++Institut für Pathologie, Technische Universität München, München, Deutschland.++Institut für Pathologie, Universitätsklinikum Köln, Köln, Deutschland | Full Paper |
2023 | Stark, GF;Martin, RM;Smith, LE;Wei, B;Hellweger, FL;Bullerjahn, GS;McKay, RML;Boyer, GL;Wilhelm, SW; | Cool temperature acclimation in toxigenic Microcystis aeruginosa PCC 7806 and its non-toxigenic mutant | bioRxiv : the preprint server for biology | 37693631 | esent. Final RNA concentrations were determined using a high-sensitivity Qubit RNA assay per manufacturer’s instructions (Invitrogen). RNA sequencing and sequence analyses RNA samples were sent to Discovery Life Sciences (previously Hudson Alpha, Huntsville, AL) where sample library prep and Illumina Novaseq 6000 platform sequencing (50 million reads, 150 bp, paired end) were performed after ri | Full Paper |
2023 | Favaro, P;Glass, DR;Borges, L;Baskar, R;Reynolds, W;Ho, D;Bruce, T;Tebaykin, D;Scanlon, VM;Shestopalov, I;Bendall, SC; | Unravelling human hematopoietic progenitor cell diversity through association with intrinsic regulatory factors | bioRxiv : the preprint server for biology | 37693547 | All the primary samples were deindentified and purchased from suppliers of healthy donors (AllCells and StemExpress). The CD34-enriched cells (CliniMACS, Miltenyi) Save | Full Paper |
2023 | Banerjee, P;Diniz, WJS;Dyce, PW; | Dataset for miRNA expression analysis in the peripheral white blood cells of beef heifers at weaning | Data in brief | 37680347 | ution for the final library was assessed by Agilent Bioanalyzer high-sensitivity DNA assay (Agilent, Santa Clara, CA, United States). The quality-checked libraries were sequenced using NextSeq 500 at Discovery Life Sciences (Hudson Alpha Institute of Biotechnology, Huntsville, AL, United States). Single-end 50 bp data was generated for each sample. 4.4 Data Analysis After sequencing, the quality | Full Paper |
2023 | Fernando, V;Zheng, X;Sharma, V;Furuta, S; | Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolism | bioRxiv : the preprint server for biology | 37662241 | btained from Karmanos Cancer Institute (Detroit, MI) under Material Transfer Agreement. Peripheral blood mononuclear cells (PBMCs) were obtained from Stemcell Technologies (Vancouver, BC, Canada) and AllCells LLC (Alameda, CA). Cell culture & Reagents THP-1 cells were maintained at a density of 1×106 cells/ml in RPMI 1640 Medium (ThermoFisher, Waltham, MA, Cat. No. 11835055) supplemented with | Full Paper |
2023 | Murgo, E;De Santis, E;Sansico, F;Melocchi, V;Colangelo, T;Padovano, C;Colucci, M;Carbone, A;Totti, B;Basti, A;Gottschlich, L;Relogio, A;Capitanio, N;Bianchi, F;Mazzoccoli, G;Giambra, V; | The circadian clock circuitry modulates leukemia initiating cell activity in T-cell acute lymphoblastic leukemia | Journal of experimental & clinical cancer research : CR | 37620852 | Interestingly, we found that the addition of recombinant hIL20 to CLOCK-overexpressing T-ALLcells induced greater expansion with respect to mock treated cells, as well Save Cached | Full Paper |
2023 | Yadavilli, S;Waight, JD;Brett, S;Bi, M;Zhang, T;Liu, YB;Ellis, C;Turner, DC;Hahn, A;Shi, H;Seestaller-Wehr, L;Jing, J;Xie, Q;Shaik, JS;Ji, X;Gagnon, R;Fieles, W;Hook, L;Grant, S;Hopley, S;DeYoung, MP;Blackwell, C;Chisamore, M;Biddlecombe, R;Figueroa, DJ;Hopson, CB;Srinivasan, R;Smothers, J;Maio, M;Rischin, D;Olive, D;Paul, E;Mayes, PA;Hoos, A;Ballas, M; | Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint Blockade | Cancer research communications | 37593752 | , 6-8-week-old female NSG mice (The Jackson Laboratory, RRID:IMSR_JAX:005557) were engrafted intravenously with 20×106 human PBMC (single donor; AllCells) Save;- Fresh PBMC for NSG TM mice engrafting were purchased from AllCells. with 20 × 10 6 human PBMC (single donor; AllCells) when tumors were approximately 100 mm 3 . Save | Full Paper |
2023 | Hemken, P;Qin, X;Sokoll, L;Jackson, L;Feng, F;Li, P;Gawel, S;Tu, B;Lin, Z;Hartnett, J;Hawksworth, D;Tieman, B;Yoshimura, T;Kinukawa, H;Ning, S;Liu, E;Meng, F;Chen, F;Miao, J;Mi, X;Tong, X;Chan, D;Davis, G; | Validation of the Novel GLAS Algorithm for Early Detection of Liver Fibrosis and Cirrhosis Based on GP73, LG2m, Age, and Sex | Research Square | x, race/ethnicity, and etiology of liver disease. An additional set of serum samples (collectively referred to as the Western Vendor Cohort, WVC) were purchased from BioIVT (Wesbury, NY), Biomex GmbH (Heidelberg, Germany), Discovery Life Sciences (Huntsville, AL), and ProMedDx (Norton, MA). These three sample sets (JHU/UTSMC/WVC) were combined to develop and train | Full Paper | |
2023 | Micco, R;Volpe, L;Vacca, R;Midena, F;Tavella, T;Naldini, M;Barcella, M;Giannetti, K;Ferrari, S;Beretta, S;Farina, G;Alessandrini, L;Santoro, A;Carsana, E;Varesi, A;Gilioli, D;Conti, A;Merelli, I;Gentner, B;Naldini, L; | p38 MAPK fuels proliferation stress and DNA damage impairing the functionality of genetically engineered hematopoietic stem and progenitor cells | Research Square | G-CSF or G-CSF + Plerixafor mPB CD34+ HSPCs were purified in-house with the CliniMACS 719 CD34 Reagent System (Miltenyi Biotec) from Mobilized Leukopak (AllCells) according to the TIGET720 HPCT protocol approved by OSR Ethical Committee and following | Full Paper | |
2023 | Bedard, P;Hernando-Calvo, A;Yang, C;Vila-Casadesús, M;Han, M;Liu, A;Berman, H;Spreafico, A;Razak, A;Lheureux, S;Hansen, A;Giacco, D;Matito, J;Pugh, T;Bratman, S;Aleshin, A;Berche, R;Saavedra, O;Garralda, E;Elston, S;Siu, L;Ohashi, P;Vivancos, A; | External validation of the VIGex gene-expression signature as a novel predictive biomarker for immune checkpoint treatment | Research Square | The level of PD-L1 staining is reported by Qualtek as a modi ed proportion score (MPS, range 0-100), indicating the percentage of PD-L1-expressing tumor cells and Save | Full Paper | |
2023 | Biffi, A;Montepeloso, A;Mattioli, D;Pellin, D;Peviani, M;Genovese, P; | Targeted gene addition at the CX3CR1 locus of human HSPCs improves their ability to repopulate the myeloid CNS compartment and drives lineage specific and robust transgene expression in their CNS progeny | Research Square | Commercial cord blood CD34+ human HSPCs were purchased frozen from AllCells and were thawed using Thymocyte Thawing Media (TTM) medium, modified from 40. Save | Full Paper | |
2023 | Schlabach, M;Lin, S;Collester, Z;Wrocklage, C;Shenker, S;Calnan, C;Xu, T;Gannon, H;Williams, L;Thompson, F;Dunbar, P;LaMothe, R;Garrett, T;Colletti, N;Hohmann, A;Tubo, N;Bullock, C;Le Mercier, I;Sofjan, K;Merkin, J;Keegan, S;Kryukov, G;Dugopolski, C;Stegmeier, F;Wong, K;Sharp, F;Cadzow, L;Benson, M; | Rational design of a SOCS1-edited tumor infiltrating lymphocyte therapy for solid tumors using CRISPR/Cas9 screens | bioRxiv | Cell therapies such as Tumor Infiltrating Lymphocyte (TIL) therapy have shown promise in the treatment of patients with refractory solid tumors, with improvement in Save;- Dissociated melanoma tumors from donor 110005746 were obtained from Conversant Bio and seeded at 1x56 cells/ml in TIL-CM (RPMI 1640 + 10% HI HS, 1X HEPES, Save | Full Paper | |
2023 | Chu, S;Soupene, E;Wienert, B;Yin, H;Sharma, D;Jia, K;Homma, S;Hampton, J;Gardner, J;Conklin, B;MacKenzie, T;Porteus, M;Cromer, M; | Dual α-globin and truncated erythropoietin receptor knock-in restores hemoglobin production in α-thalassemia major-derived hematopoietic stem and progenitor cells | bioRxiv | Alpha-thalassemia is an autosomal recessive monogenic disease with increasing worldwide prevalence. The molecular basis is due to mutation or deletion of one or more Save | Full Paper | |
2023 | Sherman, M;Michalski, J;Das, S;Yang, H;Barnoy, S;Chandrasekaran, L;Venkatesan, M;Ernst, R; | Targeted lipid A modification ofShigellavaccine strains reduced endotoxicity without compromising immunogenicity or invasiveness | bioRxiv | Peripheral blood monocytes (PBMCs) obtained from 4 independent donors (AllCells), were 730 prepared by two washes of warm cRPMI and seeded at 5 x 105 cells/well Save | Full Paper | |
2023 | Yulyaningsih, E;Suh, J;Fanok, M;Chau, R;Solanoy, H;Takahashi, R;Bakardjiev, A;Becerra, I;Benitez, N;Chiu, C;Davis, S;Dowdle, W;Earr, T;Estrada, A;Gill, A;Ha, C;Haddick, P;Henne, K;Larhammar, M;Leung, A;Maciuca, R;Memarzadeh, B;Nguyen, H;Nugent, A;Osipov, M;Ran, Y;Rebadulla, K;Roche, E;Sandmann, T;Wang, J;Lewcock, J;Scearce-Levie, K;Kane, L;Sanchez, P; | DNL343 is an investigational CNS penetrant eIF2B activator that prevents and reverses the effects of neurodegeneration caused by the Integrated Stress Response | bioRxiv | nts were obtained under a material transfer agreement with Baylor Scott & White Research Institute (Dallas, TX). Samples were collected 15-25 years prior to analysis. Plasma EDTA from healthy controls obtained from BioIVT, Allcells, and Denali Phase 1 clinical trial: NCT04268784. 885 CSF from healthy controls obtained from Biochemed and D | Full Paper | |
2023 | Golden, A;Slater, H;Jang, I;Walke, S;Phan, T;Bizilj, G;Rashid, A;Barney, B;Domingo, G; | Analytical sensitivity analysis and clinical impact modeling of Rapigen rapid diagnostics tests for malaria | medRxiv | All clinical positive samples from Discovery Life Sciences used in panels were confirmed 99 positive for Plasmodium monoinfection by photo-induced electron transfer Save | Full Paper | |
2023 | Camarena, J;Luna, S;Hampton, J;Majeti, K;Charlesworth, C;Soupene, E;Sheehan, V;Cromer, M;Porteus, M; | Using human genetics to develop strategies to increase erythropoietic output from genome-edited hematopoietic stem and progenitor cells | bioRxiv | Human genetic polymorphisms result in a diversity of phenotypes. Some sequences are pathologic and lead to monogenic diseases, while others may confer beneficial Save | Full Paper | |
2023 | Byrne, A;Le, D;Sereti, K;Menon, H;Patel, N;Lund, J;Xavier-Magalhaes, A;Shi, M;Sterne-Weiler, T;Modrusan, Z;Stephenson, W; | Single-cell long-read targeted sequencing reveals transcriptional variation in ovarian cancer | bioRxiv | Ovarian cancer dissociated tumor cells were purchased through Discovery Life Sciences (Huntsville, AL). Both sam | Full Paper | |
2023 | Fong, H;Mendel, M;Jascur, J;Najmi, L;Kim, K;Lew, G;Garimalla, S;Schock, S;Hu, J;Villegas, A;Conway, A;Fontenot, J;Zompi, S; | A Serum- and Feeder-Free System to Generate CD4 and Regulatory T Cells from Human iPSCs | bioRxiv | CD4 T cells were isolated from mobilized 413 leukapheresis material from a healthy male donor (AllCells, LLC) with informed written consent. 414 The raw material was Save | Full Paper | |
2023 | Machado, A;Pereira, I;Pereira, J;Maltez, L;Brandão, A;Alvites, R;Sousa, A;Branquinho, M;Caseiro, A;Pedrosa, S;Maurício, A;Pires, I;Prada, J;Santos, J;Gama, M; | Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repair | Materialia | Cells were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01) and cultured under standard conditi | Full Paper | |
2023 | Freitas, AVDA; | eVOLVER-construção e automação de plataforma de cultura celular e utilização de métodos alternativos para a medição de pH | Thesis | Fonte: https://www.mouser.ca/Product Detail/Qualtek/FMA1- 09225WBHW12?qs=T3oQrply3y%2FWD TeWGhtP%252BA%3D%3D, adaptado | Full Paper | |
2022 | Catapult, UK;Karydis, A;Kazanis, L; | Table of Contents | 2022 IEEE Future Networks World Forum (FNWF) | (Ericsson Inc., Canada)Federated Machine Learning Through edge Ready Architectures with Privacy Preservation as aService 347 Konstantinos Koutsopoulos (Qualtek Save | Full Paper | |
2023 | Follmann, M; | Surveillance von arboviralen Infektionen und Malaria in einem Distriktkrankenhaus in Nord-Tansania mittels molekularbiologischer Diagnostik und POC-Schnelltests | Thesis | Rift-Valley-Virus (RVFV) Robert Koch Institut, Berlin Inaktivierter Zellkulturüberstand Plasmodium falciparum Discovery Life Sciences P.fal DLS16-11865 VB P.viv DLS15-08486 Serum P.mal Serum Vollblut oder Serum von Malariapatienten, mikroskopisch überprüfte Proben mit hoher Erregerlast | Full Paper | |
2023 | Murach, A;Marquez, G;Tonn, K;Vormbaum, J; | In-Situ Mechanical Tester | Thesis | 2 131000 Bearal jack plug 1 Tensility Internation Corp CONN JACK PNL MNT 5.5X2.1MM ISO Digikey $4.97 $4.97 2 131000 AC Power Cord 1 CNC Tech CORD 5/15P - C13 18AWG BLACK 3FT Digikey $9.52 $9.52 2 Heat Shrink 1 Qualtek HEATSHRINK 3/16" X 4' BLACK Digikey $0.81 $0.81 | Full Paper | |
2023 | Houk, BE;Alvey, CW;Visswanathan, R;Kirkovsky, L;Matschke, KT;Kimoto, E;Ryder, T;Obach, RS; | Aurora Signaling | aurorasignaling.com | , Illinois), radioimmunoprecipitation assay buffer from TEKnova (Hollister, California), and cryopreserved hepatocytes (lot HH1025) from In Vitro ADMET laboratories ( Save | Full Paper | |
2023 | Sherman, ME; | Bacterial Enzymatic Combinatorial Chemistry (BECC) Enabled Targeted Lipid a Modification of Shigella Vaccine Strains to Reduce Endotoxicity Without Compromising Immunogenicity or Invasiveness | Thesis | Peripheral blood monocytes (PBMCs) were obtained from 4 independent donors (AllCells), rapidly thawed in a 37°C water bath, and diluted with 500 µL of cRPMI, dropwise. Cells were harvested by centrifugation (800 RPM, 5 minutes) a | Full Paper | |
2023 | Boccacci, Y; | Modélisation in vitro de variants génétiques dans des globules rouges dérivés de cellules souches hématopoïétiques avec CRISPR-Cas9 | Thesis | Purified mobilized peripheral blood stem cells (mPBSC) were purchased from AllCells (Alameda, CA, USA). Cord blood (CB) units were collected at the Centre Hospitalier Universitaire Saint-François d’Assise (Québec, QC, Canada), after informed consent of particip | Full Paper | |
2023 | Chu, Q;Perrone, F;Greillier, L;Tu, W;Piccirillo, MC;Grosso, F;Lo Russo, G;Florescu, M;Mencoboni, M;Morabito, A;Cecere, FL;Ceresoli, GL;Dawe, DE;Zucali, PA;Pagano, M;Goffin, JR;Sanchez, ML;Gridelli, C;Zalcman, G;Quantin, X;Westeel, V;Gargiulo, P;Delfanti, S;Tu, D;Lee, CW;Leighl, N;Sederias, J;Brown-Walker, P;Luo, Y;Lantuejoul, S;Tsao, MS;Scherpereel, A;Bradbury, P;Laurie, SA;Seymour, L; | Pembrolizumab plus chemotherapy versus chemotherapy in untreated advanced pleural mesothelioma in Canada, Italy, and France: a phase 3, open-label, randomised controlled trial | Lancet (London, England) | 37931632 | Background Pleural mesothelioma usually presents at an advanced, incurable stage. Chemotherapy with platinum-pemetrexed is a standard treatment. We hypothesised Save | Full Paper |
2023 | Ackerman, L;Acloque, G;Bacchelli, S;Schwartz, H;Feinstein, BJ;La Stella, P;Alavi, A;Gollerkeri, A;Davis, J;Campbell, V;McDonald, A;Agarwal, S;Karnik, R;Shi, K;Mishkin, A;Culbertson, J;Klaus, C;Enerson, B;Massa, V;Kuhn, E;Sharma, K;Keaney, E;Barnes, R;Chen, D;Zheng, X;Rong, H;Sabesan, V;Ho, C;Mainolfi, N;Slavin, A;Gollob, JA; | IRAK4 degrader in hidradenitis suppurativa and atopic dermatitis: a phase 1 trial | Nature medicine | 37957373 | For selectivity analysis, isolated human PBMCs (AllCells) were treated with KT-474 at 300 nM in RPMI 1640 media supplemented with 10% FBS and penicillin- Save | Full Paper |
2023 | Baumgartner, CK;Ebrahimi-Nik, H;Iracheta-Vellve, A;Hamel, KM;Olander, KE;Davis, TGR;McGuire, KA;Halvorsen, GT;Avila, OI;Patel, CH;Kim, SY;Kammula, AV;Muscato, AJ;Halliwill, K;Geda, P;Klinge, KL;Xiong, Z;Duggan, R;Mu, L;Yeary, MD;Patti, JC;Balon, TM;Mathew, R;Backus, C;Kennedy, DE;Chen, A;Longenecker, K;Klahn, JT;Hrusch, CL;Krishnan, N;Hutchins, CW;Dunning, JP;Bulic, M;Tiwari, P;Colvin, KJ;Chuong, CL;Kohnle, IC;Rees, MG;Boghossian, A;Ronan, M;Roth, JA;Wu, MJ;Suermondt, JSMT;Knudsen, NH;Cheruiyot, CK;Sen, DR;Griffin, GK;Golub, TR;El-Bardeesy, N;Decker, JH;Yang, Y;Guffroy, M;Fossey, S;Trusk, P;Sun, IM;Liu, Y;Qiu, W;Sun, Q;Paddock, MN;Farney, EP;Matulenko, MA;Beauregard, C;Frost, JM;Yates, KB;Kym, PR;Manguso, RT; | The PTPN2/PTPN1 inhibitor ABBV-CLS-484 unleashes potent anti-tumour immunity | Nature | 37794185 | ces regulations and the International Conference on Harmonisation Guidelines. All human research participants signed informed consent forms. Blood samples from patients with cancer were acquired from Discovery Life Sciences. Data from whole blood showing occasional overt clotting after overnight culture were excluded. pSTAT flow cytometry analyses Heparinized blood samples were treated with AC484 | Full Paper |
2023 | Tsuchida, CA;Brandes, N;Bueno, R;Trinidad, M;Mazumder, T;Yu, B;Hwang, B;Chang, C;Liu, J;Sun, Y;Hopkins, CR;Parker, KR;Qi, Y;Hofman, L;Satpathy, AT;Stadtmauer, EA;Cate, JHD;Eyquem, J;Fraietta, JA;June, CH;Chang, HY;Ye, CJ;Doudna, JA; | Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cells | Cell | 37794590 | cells were originally derived from a female donor. Primary adult peripheral blood mononuclear cells Primary adult peripheral blood mononuclear cells (PBMCs) were obtained as cryopreserved vials from Allcells Inc. CD3+ T cells were isolated from PBMCs using EasySep Human T Cell Isolation Kits (StemCell Technologies) according to the manufacturer’s instructions. Isolated CD3+ T cells were culture | Full Paper |
2023 | Ligero, M;El Nahhas, O;Prior Palomares, O;Navarro, V;Sansano, I;Serna, G;Mauchanski, S;Toledo, R;Dienstmann, R;Ramon y Cajal, S;Garralda, E;Nuciforo, P;Kather, J;Perez Lopez, R; | 176P Enhancing immunotherapy response prediction via multimodal integration of radiology and pathology deep learning models | Annals of Oncology | Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. J.N. Kather: Financial Interests, Personal, Invited Speaker: Fresenius, Eisai, MSD; Financial Interests, Personal, Advisory Board: Owkin, DoMore Diagnostics, Panakeia, London, UK. R. Perez Lopez: Financial Interests, Personal, Full or part-time Employment, VHIO staff (team leader of the Radiomics Group): VHIO; Financial Interests, Institutional, Research Grant, | Full Paper | |
2023 | Jové, M;Braña, I;Oliva Bernal, M;Hernando Calvo, A;Erasun Lecuona, C;Assaf Pastrana, J;Mato-Berciano, A;Maliandi, M;Torres-Manjon, S;Moreno, R;Le, C;Nuciforo, P;Alemany, R;Capella, G;Blasco, C;Cascallo Piqueras, M;Mesia Nin, R; | 937P Survival outcomes in phase I trial combining VCN-01 and durvalumab (MEDI4736) in subjects with recurrent/metastatic head and neck squamous cell carcinoma refractory to previous immunotherapy treatment | Annals of Oncology | rsonal, Advisory Board: BAYER, MSD ONCOLOGY; Financial Interests, Personal, Invited Speaker: NOVARTIS; Financial Interests, Personal, Other, Consultant: TARGOS Molecular Pathology. R. Alemany: Financial Interests, Personal, Stocks/Shares: Theriva Biologics; Financial Interests, Institutional, Research Funding, T: Theriva Biologics SL; Financial Interests, Institutional, Speaker, Consultant, Advisor: Theriva Biologics SL. G. Capella: Financial Interests, Personal, Other, Consultancy: THERIVA BIOLOGICS; Financial Interests, Personal, Stocks/Shares: THERIVA BIOLOGICS; NonFinancial Interests, Member of Board of Directors, Chairperson 2022-2024: International Society for the Study of Hereditary Gastrointestinal Cancer; Non-Financial Interests, Member of Board of Directors: FUREGA Fundació Per la Recerca en Gastroenterologia; Non-Financial Interests, Member: ASCO, EACR-ASEICA, EHTG European Hereditary Tumor Group. C. Blasco: Financial I | Full Paper | |
2023 | Haubner, S;Mansilla-Soto, J;Nataraj, S;Kogel, F;Chang, Q;de Stanchina, E;Lopez, M;Ng, MR;Fraser, K;Subklewe, M;Park, JH;Wang, X;Rivière, I;Sadelain, M; | Cooperative CAR targeting to selectively eliminate AML and minimize escape | Cancer cell | 37802054 | Normal donor whole bone marrow, fresh Allcells N/A Buffy coats New York Blood Center N/A AML patient samples MSKCC IRB# 06-107 and IRB# 14-091 AML patient-derived xenograft mode | Full Paper |
2023 | Lorenzen, S;Götze, TO;Thuss-Patience, P;Biebl, M;Homann, N;Schenk, M;Lindig, U;Heuer, V;Kretzschmar, A;Goekkurt, E;Haag, GM;Riera-Knorrenschild, J;Bolling, C;Hofheinz, RD;Zhan, T;Angermeier, S;Ettrich, TJ;Siebenhuener, AR;Elshafei, M;Bechstein, WO;Gaiser, T;Loose, M;Sookthai, D;Kopp, C;Pauligk, C;Al-Batran, SE;AIO and SAKK Study Working Groups, ; | Perioperative Atezolizumab Plus Fluorouracil, Leucovorin, Oxaliplatin, and Docetaxel for Resectable Esophagogastric Cancer: Interim Results From the Randomized, Multicenter, Phase II/III DANTE/IKF-s633 Trial | Journal of clinical oncology : official journal of the American Society of Clinical Oncology | 37963317 | Honoraria: Servier, MSD Oncology, Lilly, Bristol Myers Squibb, Targos GmbH, iOMEDICO, MCI Conventions, AstraZeneca Consulting or Advisory Role: Bristol Myers Squibb (Inst), MSD Oncology (Inst), Lilly, Novartis, Daiichi Sankyo Europe GmbH, Servier, Pierre Fabre, AstraZeneca Research Funding: Taiho Pharmaceutical (Inst), Nordic Group (Inst), MSD Oncology (Inst) Travel, Accommodations, Expense | Full Paper |
2023 | Sun, Y;Yang, XN;Yang, SS;Lyu, YZ;Zhang, B;Liu, KW;Li, N;Cui, JC;Huang, GX;Liu, CL;Xu, J;Mi, JQ;Chen, Z;Fan, XH;Chen, SJ;Chen, S; | Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicity | Signal transduction and targeted therapy | 38062078 | edia (Thermo Fisher 10743029). All cell lines were cultured at 37?°C, 5% CO2. Human samples The healthy donor peripheral blood mononuclear cells (PBMCs) were obtained from HemaCare Corporation or AllCells. Identification and engineering of the nanobody tandem We immunized two llamas with purified BCMA, yielding a high-volume nanobody phage display library from which we isolated and sequenced | Full Paper |
2023 | Nolan-Stevaux, O;Li, C;Liang, L;Zhan, J;Estrada, J;Osgood, T;Li, F;Zhang, H;Case, R;Murawsky, CM;Estes, B;Moore, GL;Bernett, MJ;Muchhal, U;Desjarlais, JR;Staley, BK;Stevens, J;Cooke, KS;Aeffner, F;Thomas, O;Stieglmaier, J;Lee, JL;Coxon, A;Bailis, JM; | AMG 509 (Xaluritamig), an Anti-STEAP1 XmAb 2+1 T-cell Redirecting Immune Therapy with Avidity-Dependent Activity Against Prostate Cancer | Cancer discovery | 37861452 | ation using STAT-Myco testing (IDEXX Bioanalytics). Effector cells were human T cells (AllCells) or human or cynomolgus monkey PBMCs (SNBL) | Full Paper |
2023 | Kwok, SJJ;Forward, S;Fahlberg, MD;Assita, ER;Cosgriff, S;Lee, SH;Abbott, GR;Zhu, H;Minasian, NH;Vote, AS;Martino, N;Yun, SH; | High-dimensional multi-pass flow cytometry via spectrally encoded cellular barcoding | Nature biomedical engineering | 38036616 | Anti-CD45 antibodies conjugated to the 14 fluorophores were used to stain freshly thawed human PBMCs (AllCells). Cells (10 6 ) were photobleached in 2 ml of 'wash Save | Full Paper |
2023 | Olson, TS;Walters, MC; | Allogeneic haematopoietic stem-cell transplantation versus gene therapy for haemoglobinopathies | The Lancet. Haematology | 37793770 | MCW has received consulting fees from AllCells Incorporated and BioChips Incoroporated; payment or honoraria for lectures from TouchIME; and is on the scientific advisory board for Ensoma Incorporated and the steering committee for Vertex Pharmaceuticals In | Full Paper |
2023 | Han, W;Qiu, HY;Sun, S;Fu, ZC;Wang, GQ;Qian, X;Wang, L;Zhai, X;Wei, J;Wang, Y;Guo, YL;Cao, GH;Ji, RJ;Zhang, YZ;Ma, H;Wang, H;Zhao, M;Wu, J;Bi, L;Chen, QB;Li, Z;Yu, L;Mou, X;Yin, H;Yang, L;Chen, J;Yang, B;Zhang, Y; | Base editing of the HBG promoter induces potent fetal hemoglobin expression with no detectable off-target mutations in human HSCs | Cell stem cell | 37989316 | processed according to the protocol of Miltenyi CD34 Microbead kit (Miltenyi Biotec). The genotypes of the three b0 /b0 donors as shown: the first donor: aa-4.2/aa, bCD41-42(-TTCT)/bCD41-42(-TTCT); the second donor: aa-3.7/aa, bCD41-42(-TTCT)/bCD41-42(-TTCT); the third donor: aa/a-3.7aHb Westmead, bCD41-42(-TTCT)/bCD41-42(-TTCT). Frozen healthy CD34+ cells were purchased from Allcells and SAILY BIO (China) | Full Paper |
2023 | Kassim, A;Walters, M;Eapen, M;Ritzau, N;Smith, M;Solh, M;McKinney, C;Nieder, M;Ross, M;Kent, M;Abusin, G;Mallhi, K;Silva, J;Shaughnessy, P;Kanter, J;Haines, H;Farah, R;Khaled, Y;Abraham, A;Bollard, C;Cooke, K;de La Fuente, J;Hanna, R;Horowitz, M;Jordan, L;Krishnamurti, L;Leifere, E;Mahadeo, K;Shenoy, S;Ritzau, N;DeBaun, M;Brodsky, R; | Reduced Intensity Haploidentical Bone Marrow Transplantation in Adults with Severe Sickle Cell Disease: BMT CTN 1507 | Blood | Background: Allogeneic hematopoietic stem-cell transplantation has curative potential for sickle cell disease (SCD). Event-free survival (EFS) in children with SCD is >90% Save | Full Paper | |
2023 | Frangoul, H;Locatelli, F;Sharma, A;Bhatia, M;Mapara, M;Molinari, L;Wall, D;Liem, R;Telfer, P;Shah, A;Cavazzana, M;Corbacioglu, S;Rondelli, D;Meisel, R;Dedeken, L;Lobitz, S;de Montalembert, M;Steinberg, M;Walters, M;Bower, L;Imren, S;Simard, C;Xuan, F;Zhou, W;Morrow, P;Hobbs, W;Grupp, S; | Exagamglogene Autotemcel for Severe Sickle Cell Disease | Blood | maceuticals: Consultancy; BioChip Labs: Consultancy, Other: Medical Director; AllCells, Inc: Consultancy, Other: Medical Director. Bower: Vertex Pharmaceuticals: Current Employment. Imren: Vertex Pharmaceuticals: Current Employment, Current holder of stock options in a privately-held company. Simard: Vertex Pharmaceuticals: Current Employment. Xuan: Vertex Pharmaceuticals: Current Employment. Zhou: Vertex Pharmaceuticals: Current Employment. Morrow: Vertex Pharmaceuticals: Membership on an en | Full Paper | |
2023 | Hanna, R;Frangoul, H;McKinney, C;Pineiro, L;Mapara, M;Dalal, J;Chang, K;Jaskolka, M;Kim, K;Farrington, D;Wally, M;Mei, B;Lawal, A;Afonja, O;Walters, M; | AsCas12a Gene Editing of HBG1/2 Promoters with EDIT-301 Results in Rapid and Sustained Normalization of Hemoglobin and Increased Fetal Hemoglobin in Patients with Severe Sickle Cell Disease and Transfusion-Dependent Beta-Thalassemia | Blood | oyment. Walters: AllCells, Inc: Consultancy, Other: Medical Director; BioChip Labs: Consultancy, Other: Medical Director; Vertex Pharmaceuticals: Consultancy; Ensoma, Inc: | Full Paper | |
2023 | Huang, J;Gu, H;Orton, J;Sedova, M;Marcovitz, A;Burke, J;Brozio, S;Williams, P;Myrand, S;Olowo, N;Broomer, A;Deal, B;Seeger, C;Sadis, S;Rozenzhak, S;Hyland, F;Liu, G; | Detection of KMT2A Partial Tandem Duplications ( KMT2A-PTDs) in Healthy Donors Using Next Generation Sequencing | Blood | Introduction KMT2A ( MLL) fusions are relevant biomarkers in myeloid malignancies. KMT2A-PTD (also called MLL-PTD, partial tandem duplication), a non-fusion gene rearrangement, also occurs and serves as an important biomarker for Menin-inhibitors. Traditionally, KMT2A-PTDs are detected by RT-qPCR (quantitative real-time PCR). Here we report the detection of KMT2A-PTDs in whole blood samples taken from healthy donors using the Oncomine TM Myeloid Assay GX v2, which is a next-generation sequencing (NGS) based assay that enables efficient detection of multiple genetic alterations in parallel. KMT2A-PTD positive clinical research samples were used as a control in this study. This study was initiated based on reports from various healthcare professionals who observed several healthy blood donors identified with KMT2A-PTD using the Oncomine TM Myeloid Assay GX v2. As a result, we conducted tests on healthy controls and compared them with clinical research samples known to have myeloid malignancies to explore this genomic alteration further. Methods We sequenced 20 whole blood samples (total 104 replicates) from healthy donors at 3 different sites of Thermo Fisher Scientific TM (South San Francisco, CA; Guilford, CT; Carlsbad, CA). These whole blood samples were received from Stanford Blood Center or purchased from Discovery Life Sciences. As a control, we also sequenced 20 clinical research samples (total 85 replicates) that are known to have myeloid malignancies in both Sonora Quest Laboratories and at Thermo Fisher Scientific TM South San Francisco site. These samples were run on the Ion Torrent TM Genexus TM Software 6.6 and analyzed using the Oncomine TM Myeloid Assay GX v2 with fusion profiling, where the assay design targets 6 different KMT2A-PTD variants. Results The data set has mean read length 90 - 120bp and total mapped fusion reads 20,000 - 30,000. We have found evidence of KMT2A-PTDs in confirmed positive samples from myeloid malignancies (including: AML, acute myeloid leukemia; MPN, myeloproliferative neoplasms; MDS, myelodysplastic syndromes; CML, chronic myeloid leukemia; CMML, chronic myelomonocytic leukemia; or JMML, juvenile myelomonocytic leukemia) and healthy donor samples. The read counts of PTDs detected in the healthy donor samples are on average about 1/3 of the read counts of PTDs detected in the myeloid positive samples, but the distributions overlap. Approximately 33% of myeloid positive samples have PTD read counts higher than the maximum read counts detected in any of the healthy donor samples. The sequences of KMT2A-PTDs found in both healthy donor samples and myeloid samples were further analyzed in BLAT (BLAST-Like Alignment Tool) to confirm that they match the specific exons on the KMT2A gene. Based on this data, only samples with myeloid malignancies have >2000 reads that support KMT2A-PTDs with this assay. Conclusions We describe the detection of KMT2A-PTDs in a range of clinical research samples, including healthy controls as well as samples with myeloid malignancies. KMT2A-PTDs were detected in healthy control samples at lower levels, however, one third of myeloid samples showed significantly higher KMT2A-PTD read counts compared to healthy controls. This intriguing finding warrants further investigation, as it opens up the possibility of conducting a prospective study to follow up on these individuals and observe if they develop myeloid malignancies. Moreover, this finding presents an opportunity for conducting retrospective studies to explore whether healthy controls previously identified with this alteration several years ago after blood donation were subsequently recorded in the health national system with myeloid malignancies. (For research use only. Not for use in diagnostic procedures.);- These whole blood samples were received from Stanford Blood Center or purchased from Discovery Life Sciences. As a control, we also sequenced 20 clinical research Save | Full Paper | |
2023 | Kanter, J;Thompson, A;Kwiatkowski, J;Parikh, S;Mapara, M;Rifkin-Zenenberg, S;Aygun, B;Kasow, K;Gupta, A;Zhang, L;Sheldon-Waniga, E;Gallagher, M;Gruppioni, K;Chawla, A;Elliot, H;Pierciey, F;Walters, M;Tisdale, J; | Efficacy, Safety, and Health-Related Quality of Life (HRQOL) in Patients with Sickle Cell Disease (SCD) Who Have Received Lovotibeglogene Autotemcel (Lovo-cel) Gene Therapy: Up to 60 Months of Follow-up | Blood | Employment, Current equity holder in private company. Walters: AllCells, Inc: Consultancy, Other: Medical Director; BioChip Labs: Consultancy, Other: Medical Director; Ensoma, Inc: Consultancy; Vertex Pharmaceuticals: Consultan | Full Paper | |
2023 | Thompson, A;Olson, T;Walters, M;Porter, J;Schneiderman, J;Hongeng, S;Kulozik, A;Cavazzana, M;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Rasko, J;Yannaki, E;Ali, S;Tao, G;Thakar, H;Deora, A;Gruppioni, K;Colvin, R;Locatelli, F;Kwiatkowski, J; | Sustained Efficacy, Safety, and Improved Quality of Life in Adult and Pediatric Patients with Transfusion-Dependent ?-Thalassemia up to 9 Years Post Treatment with Betibeglogene Autotemcel (Beti-cel) | Blood | ics: Other: DSMB; Vertex Pharmaceuticals: Consultancy, Speakers Bureau. Walters: AllCells, Inc: Consultancy, Other: Medical Director; BioChip Labs: Consultancy, Other: Medical Director; Ensoma, Inc: Consultancy; Vertex Pharmaceuticals: Consultancy. Porter: BMS, Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees; Celgene, bluebird bio, Agios: Consultancy, Honoraria; Protag | Full Paper | |
2023 | Kwiatkowski, J;Olson, T;Walters, M;Porter, J;Schneiderman, J;Hongeng, S;Kulozik, A;Cavazzana, M;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Rasko, J;Yannaki, E;Ali, S;Tao, G;Deora, A;Thakar, H;Colvin, R;Locatelli, F;Thompson, A; | Improvement in Iron Burden in Patients with Transfusion-Dependent ?-Thalassemia (TDT) Treated with Betibeglogene Autotemcel (Beti-cel) Gene Therapy: Up to 9 Years of Follow-up | Blood | vately-held company; Vertex Pharmaceuticals: Membership on an entity's Board of Directors or advisory committees; BioLabs, Inc, AllCells, Inc: Consultancy. Porter: BMS, Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees; Protagonism, VIFOR, Silence Therapeutics, La Jolla Pharmaceuticals: Honoraria; Celgene, bluebird bio, Agios: Consultancy, Honoraria; Silence Therapeutics: Honoraria, Membership on an entity's Board of Directors or adviso | Full Paper | |
2023 | Janesick, A;Shelansky, R;Gottscho, AD;Wagner, F;Williams, SR;Rouault, M;Beliakoff, G;Morrison, CA;Oliveira, MF;Sicherman, JT;Kohlway, A;Abousoud, J;Drennon, TY;Mohabbat, SH;10x Development Teams, ;Taylor, SEB; | High resolution mapping of the tumor microenvironment using integrated single-cell, spatial and in situ analysis | Nature communications | 38114474 | -26 and obtained from Discovery Life Sciences. Corresponding dissociated tumor cells, -07-24 and obtained from Discovery Life Sciences. 5 ?m sections were taken from Save;ple collection Sample #1. A single formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue block (TNM stage T2N1M0, ER?+?/HER2?+?/PR??) was collected on 2021-07-26 and obtained from Discovery Life Sciences. Corresponding dissociated tumor cells, fresh frozen in liquid nitrogen, were also sampled from the same biopsy (patient matched). 5 ?m sections were taken from the FFPE tissu | Full Paper |
2023 | Sun, D;Sun, Y;Janezic, E;Zhou, T;Johnson, M;Azumaya, C;Noreng, S;Chiu, C;Seki, A;Arenzana, TL;Nicoludis, JM;Shi, Y;Wang, B;Ho, H;Joshi, P;Tam, C;Payandeh, J;Comps-Agrar, L;Wang, J;Rutz, S;Koerber, JT;Masureel, M; | Structural basis of antibody inhibition and chemokine activation of the human CC chemokine receptor 8 | Nature communications | 38040762 | Human colorectal DTC (Discovery Life Sciences) were thawed according to the vendor's protocol. Human PBMCs were isolated by Ficoll gradient centrifugation from buffy Save | Full Paper |
2023 | Schlabach, MR;Lin, S;Collester, ZR;Wrocklage, C;Shenker, S;Calnan, C;Xu, T;Gannon, HS;Williams, LJ;Thompson, F;Dunbar, PR;LaMothe, RA;Garrett, TE;Colletti, N;Hohmann, AF;Tubo, NJ;Bullock, CP;Le Mercier, I;Sofjan, K;Merkin, JJ;Keegan, S;Kryukov, GV;Dugopolski, C;Stegmeier, F;Wong, K;Sharp, FA;Cadzow, L;Benson, MJ; | Rational design of a SOCS1-edited tumor-infiltrating lymphocyte therapy using CRISPR/Cas9 screens | The Journal of clinical investigation | 38099496 | Cell therapies such as Tumor Infiltrating Lymphocyte (TIL) therapy have shown promise in the treatment of patients with refractory solid tumors, with improvement in Save;- Dissociated melanoma tumors from donor 110005746 were obtained from Conversant Bio and seeded at 1x56 cells/ml in TIL-CM (RPMI 1640 + 10% HI HS, 1X HEPES, Save | Full Paper |
2023 | Zhang, L;Lee, M;Maslov, AY;Montagna, C;Vijg, J;Dong, X; | Analyzing somatic mutations by single-cell whole-genome sequencing | Nature protocols | 37996541 | was covered by Einstein-Montefiore Institutional Review Board, Lonza Walkersville Inc., Kerafast Inc., AllCells, and STEMCELL Technologies | Full Paper |
2023 | Corbacioglu, S;Frangoul, H;Locatelli, F;Hobbs, W;Walters, M; | Defining curative endpoints for transfusion-dependent ?-thalassemia in the era of gene therapy and gene editing | American journal of hematology | 38100154 | armaceuticals Incorporated. William Hobbs is an employee of Vertex Pharmaceuticals Incorporated and holds stock and/or stock options at the company. Mark Walters has served as a consultant for AllCells, Inc., BioChip, Inc., Ensoma, Inc., and Editas Medicine; served on an advisory board for Vertex Pharmaceuticals Incorporated; and holds stock/stock options in Ensoma, Inc | Full Paper |
2023 | Locatelli, F;Corbacioglu, S;Hobbs, W;Frangoul, H;Walters, MC; | Defining curative endpoints for sickle cell disease in the era of gene therapy and gene editing | American journal of hematology | 38010293 | mployee of Vertex Pharmaceuticals Incorporated and holds stock in the company. Mark Walters has served as a consultant for AllCells, Inc., BioChip, Inc., Ensoma, Inc., and Editas Medicine; served on an advisory board for Vertex Pharmaceuticals Incorporated; and holds stock/stock options in Ensoma, Inc. Open Research | Full Paper |
2023 | Guo, Q;Zhang, J;Parikh, K;Brinkley, A;Lin, S;Zakarian, C;Pernet, O;Shimizu, S;Khamaikawin, W;Hacke, K;Kasahara, N;An, DS; | In vivo selection of anti-HIV-1 gene-modified human hematopoietic stem/progenitor cells to enhance engraftment and HIV-1 inhibition | Molecular therapy : the journal of the American Society of Gene Therapy | 38087779 | microbead isolation kit (Miltenyi Biotec, Auburn, CA). Human mPB CD34+ cells were isolated from granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood leukapheresis packs purchased from ALLCELLS (Alameda, CA) using anti-CD34+ monoclonal antibodies (Miltenyi Biotec) and cryopreserved in Bambanker (GC Lymphotec, Tokyo, Japan) | Full Paper |
2023 | Wilkerson, AD;Parthasarathy, PB;Stabellini, N;Mitchell, C;Pavicic, PG;Fu, P;Rupani, A;Husic, H;Rayman, PA;Swaidani, S;Abraham, J;Budd, GT;Moore, H;Al-Hilli, Z;Ko, JS;Baar, J;Chan, TA;Alban, T;Diaz-Montero, CM;Montero, AJ; | Phase II Clinical Trial of Pembrolizumab and Chemotherapy Reveals Distinct Transcriptomic Profiles by Radiologic Response in Metastatic Triple-Negative Breast Cancer | Clinical cancer research : an official journal of the American Association for Cancer Research | 37882661 | Purpose A single arm, Phase II trial of carboplatin, nab-paclitaxel, and pembrolizumab (CNP) in metastatic triple negative breast cancer (mTNBC) was designed to Save | Full Paper |
2023 | Cash, A;de Jager, C;Brickler, T;Soliman, E;Ladner, L;Kaloss, AM;Zhu, Y;Pridham, KJ;Mills, J;Ju, J;Basso, EKG;Chen, M;Johnson, Z;Sotiropoulos, Y;Wang, X;Xie, H;Matson, JB;Marvin, EA;Theus, MH; | Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2/Akap12 signaling to preserve blood-brain barrier integrity | Proceedings of the National Academy of Sciences of the United States of America | 37796990 | proved by Virginia Tech’s IRB #19-470. Legally authorized representatives provided written informed consent. Subjects aged 18 to 50 y had labs drawn at 24, 48, and 72 h following hospital admission. Control samples were purchased to match age, sex, and race (Discovery Life Sciences). ELISAs were conducted on serum samples to quantify Ang2 expression according to the manufacturer’s instructions (Boster Bio) | Full Paper |
2023 | Schmidt, F;Fields, HF;Purwanti, Y;Milojkovic, A;Salim, S;Wu, KX;Simoni, Y;Vitiello, A;MacLeod, DT;Nardin, A;Newell, EW;Fink, K;Wilm, A;Fehlings, M; | In-depth analysis of human virus-specific CD8+ T cells delineates unique phenotypic signatures for T cell specificity prediction | Cell reports | 37837618 | PBMCs (from whole blood) Discovery Life Sciences N/A PBMCs (from whole blood) San Diego Blood Bank N/A (Continued | Full Paper |
2023 | Song, J;Lv, H;Liu, B;Hao, M;Taylor, HS;Zhang, X;Li, D;Huang, Y; | Let-7 suppresses liver fibrosis by inhibiting hepatocyte apoptosis and TGF-? production | Molecular metabolism | 37898449 | 111917V1, Lot# HH1161, 35 years old African American male, BMI 32) were purchased from Discovery Life Sciences. Cells were thawed using 37 °C Universal Cryopreservation Recovery Media (UCRM, 81015, Discovery Life Sciences) and seeded in Universal Primary Cell Plating Media (UPCM, 81016, Discovery Life Sciences). 2.7 Transfection of primary hepatocytes For let-7a transfection experiments, cells | Full Paper |
2023 | Copertino, DC;Holmberg, CS;Weiler, J;Ward, AR;Howard, JN;Levinger, C;Pang, AP;Corley, MJ;Dündar, F;Zumbo, P;Betel, D;Gandhi, RT;McMahon, DK;Bosch, RJ;Linden, N;Macatangay, BJ;Cyktor, JC;Eron, JJ;Mellors, JW;Kovacs, C;Benko, E;Bosque, A;Jones, RB;AIDS Clinical Trials Group (ACTG) A5321 Team, ; | The latency-reversing agent HODHBt synergizes with IL-15 to enhance cytotoxic function of HIV-specific T cells | JCI insight | 37581929 | an Attune NxT Flow Cytometer (Thermo Fisher Scientific) immediately. Data were analyzed using FlowJo software. Infected cell degranulation assay PBMCs from deidentified HLA-B58+ HIV-negative donors (AllCells) were depleted of CD8+ T cells (STEMCELL Technologies, EasySep, catalog 17853) and activated with ImmunoCult Human CD3/CD28 T Cell Activator (STEMCELL Technologies, catalog 10971) (for 72 hou | Full Paper |
2023 | Cahyanto, A;Rath, P;Teo, TX;Tong, SS;Malhotra, R;Cavalcanti, BN;Lim, LZ;Min, KS;Ho, D;Lu, WF;Rosa, V; | Designing Calcium Silicate Cements with On-Demand Properties for Precision Endodontics | Journal of dental research | 37861249 | applications. To this end, the cytocompatibility, gene expression, and mineralization potential were tested using dental pulp stem cells (DPSCs, passages 5-8) (DP003F; AllCells). Cells were subcultured in basal growth media (Dulbecco’s modified Eagle’s medium, penicillin-streptomycin, and 10% fetal bovine serum, all from Invitrogen). The cement eluents were prepared following ISO 10993-12:2021 (ISO 2021) in basal growth media (specimen thickness: 1.0 m | Full Paper |
2023 | Walseng, E;Wang, B;Yang, C;Patel, P;Zhao, C;Zhang, H;Zhao, P;Mazor, Y; | Conformation-selective rather than avidity-based binding to tumor associated antigen derived peptide-MHC enables targeting of WT1-pMHC low expressing cancer cells by anti-WT1-pMHC/CD3 T cell engagers | Frontiers in immunology | 38022650 | Primary AML cells were purchased as frozen cells from Discovery Life Sciences and used directly in the assay upon thawing and resting overnight. Save | Full Paper |
2023 | Meyer, MA;Dinh, HQ;Alimadadi, A;Araujo, DJ;Chatterjee, N;Gutierrez, NA;Zhu, YP;Hunter, EL;Liang, S;Seumois, G;Kiosses, WB;Catz, SD;Vijayanand, P;Ottensmeier, C;Hedrick, CC; | Human CD79b+ neutrophils in the blood are associated with early-stage melanoma | Frontiers in immunology | 38022639 | Fresh bone marrow collected from anonymous healthy donors was obtained from AllCells, Inc. (Alameda, CA) and shipped overnight on a cold pack. Heparin was used as Save | Full Paper |
2023 | Smith, AJ;Thurman, RE;Zeng, W;Grogan, B;Lucas, S;Gutierrez, G;Heiser, RA;Wo, SW;Blackmarr, A;Peterson, S;Gardai, SJ; | Nonfucosylation of an anti-TIGIT antibody enhances Fc?R engagement, driving innate immune activation and antitumor activity | Frontiers in immunology | 38022590 | For internally generated scRNA-seq data, dissociated primary human lung tumors (DTCs) from three patients were obtained from Discovery Life Sciences (DLS), who Save;- Cryopreserved PBMCs from normal healthy donors (purchased from Astarte and Folio Conversant) were thawed, washed in RPMI 1640, then washed with PBS, stained Save;g from peripheral blood or normal tissues were excluded from the analysis. For internally generated scRNA-seq data, dissociated primary human lung tumors (DTCs) from three patients were obtained from Discovery Life Sciences (DLS), who isolated T cells and performed scRNA sequencing and data analysis using the methodology described in the Supplementary Materials and Methods. Fc?R cell binding Ant | Full Paper |
2023 | Branella, GM;Lee, JY;Okalova, J;Parwani, KK;Alexander, JS;Arthuzo, RF;Fedanov, A;Yu, B;McCarty, D;Brown, HC;Chandrakasan, S;Petrich, BG;Doering, CB;Spencer, HT; | Ligand-based targeting of c-kit using engineered ?? T cells as a strategy for treating acute myeloid leukemia | Frontiers in immunology | 38022523 | al blood mononuclear cells (PBMCs) were isolated using a Ficoll-Plaque PLUS density gradient (GE HealthCare, Chicago, IL, USA) and cryopreserved. Alternatively, cryopreserved PBMCs were procured from AllCells (Alameda, CA, USA). T cells were negatively selected from thawed PBMCs using the EasySep Human T cell Isolation Kit (Stemcell Technologies, Vancouver, BC, CA) and stimulated with anti-CD3/CD2 | Full Paper |
2023 | Noe, P;Wang, JH;Chung, K;Cheng, Z;Field, JJ;Shen, X;Cortesio, CL;Pastuskovas, CV;Phee, H;Tarbell, KV;Egen, JG;Casbon, AJ; | Therapeutically targeting type I interferon directly to XCR1+ dendritic cells reveals the role of cDC1s in anti-drug antibodies | Frontiers in immunology | 37942313 | Aliquots of single cell suspensions of human dissociated ovarian tumors (serous carcinoma) were purchased from Conversant Biologics (Discovery Life Sciences) and pre- Save | Full Paper |
2023 | Garman, B;Jiang, C;Daouti, S;Kumar, S;Mehta, P;Jacques, MK;Menard, L;Manjarrez-Orduno, N;Dolfi, S;Mukherjee, P;Rai, SC;Lako, A;Koenitzer, JD;David, JM; | Comprehensive immunophenotyping of solid tumor-infiltrating immune cells reveals the expression characteristics of LAG-3 and its ligands | Frontiers in immunology | 37795090 | Human tumor and whole blood samples were commercially procured from Avaden BioSciences (WA, USA), BioOptions (CA, USA), Discovery Life Sciences (CA, USA), or MT Group (CA, USA). For the CyTOF cohort, a total of 28 tumor samples from 7 tumors types and 7 matched PBMC samples were profiled. For comparison, PBMCs from 10 healthy donors, isolated from whole blood obtained from the BMS internal blood donor program (n=8) or leukopaks commercially procured from Biospecimen Solutions, Inc. (CA, USA) ( | Full Paper |
2023 | Frey, B;Holzinger, D;Taylor, K;Ehrnhoefer, DE;Striebinger, A;Biesinger, S;Gasparini, L;O'Neill, MJ;Wegner, F;Barghorn, S;Höglinger, GU;Heym, RG; | Tau seed amplification assay reveals relationship between seeding and pathological forms of tau in Alzheimer's disease brain | Acta neuropathologica communications | 37964332 | research institute (Arizona, USA), Discovery life sciences (Alabama, USA), Folio Biosciences (by now merged to discovery life sciences). All materials were collected from Save;3, S4), including Tissue Solutions Ltd (Glasgow, UK), Analytical Biological Services Inc. (Delaware, USA), Banner sun health research institute (Arizona, USA), Discovery life sciences (Alabama, USA), Folio Biosciences (by now merged to discovery life sciences). All materials were collected from donors for whom a written informed consent for a brain autopsy and the use of the material and clinical ... 3, S4), including Tissue Solutions Ltd (Glasgow, UK), Analytical Biological Services Inc. (Delaware, USA), Banner sun health research institute (Arizona, USA), Discovery life sciences (Alabama, USA), Folio Biosciences (by now merged to discovery life sciences). All materials were collected from donors for whom a written informed consent for a brain autopsy and the use of the material and clinical ... 3, S4), including Tissue Solutions Ltd (Glasgow, UK), Analytical Biological Services Inc. (Delaware, USA), Banner sun health research institute (Arizona, USA), Discovery life sciences (Alabama, USA), Folio Biosciences (by now merged to discovery life sciences). All materials were collected from donors for whom a written informed consent for a brain autopsy and the use of the material and clinical;sources (Additional file 1: Tables S2, S3, S4), including Tissue Solutions Ltd (Glasgow, UK), Analytical Biological Services Inc. (Delaware, USA), Banner sun health research institute (Arizona, USA), Discovery life sciences (Alabama, USA), Folio Biosciences (by now merged to discovery life sciences). All materials were collected from donors for whom a written informed consent for a brain autopsy a | Full Paper |
2023 | Stark, GF;Martin, RM;Smith, LE;Wei, B;Hellweger, FL;Bullerjahn, GS;McKay, RML;Boyer, GL;Wilhelm, SW; | Microcystin aids in cold temperature acclimation: Differences between a toxic Microcystis wildtype and non-toxic mutant | Harmful algae | 37951605 | RNA samples were sent to Discovery Life Sciences (previously Hudson Alpha, Huntsville, AL) where sample library prep and Illumina Novaseq 6000 platform sequencing (50 million reads, 150 bp, paired end) were performed after ribosomal RNA reduction. A TruSeq rRNA reduction was performed on all samples ex-silico, according to manuf | Full Paper |
2023 | Yang, L;Zhang, T;Wang, P;Chen, W;Liu, W;He, X;Zhang, Y;Jin, S;Luo, Z;Zhang, Z;Wang, X;Liu, J; | Imatinib and M351-0056 enhance the function of VISTA and ameliorate the development of SLE via IFN-I and noncanonical NF-?B pathway | Cell biology and toxicology | 37804401 | The viability of Jurkat (Clone E6-1) cells or human peripheral blood mononuclear cells (PBMCs) (AllCells) cultured with compound imatinib was evaluated with CCK-8 ?it ( Save | Full Paper |
2023 | Wang, M;Moore, A;Grego, A;Cowart, M;Rager, J;Paris, G;Ito, K;Babbar, N;Geeganage, S;Scherle, P;Bhagwat, N; | Abstract C013: Clinical biomarkers based on PK/PD modeling to guide the development for a first-in-class, highly selective SMARCA2 (BRM) degrader, PRT3789 | Molecular Cancer Therapeutics | Background ? The SWI/SNF family of chromatin-remodeling complexes is frequently dysregulated in multiple tumor types, resulting in aberrant expression of genes. Save | Full Paper | |
2023 | Osborne, MJ;Sulekha, A;Culjkovic-Kraljacic, B;Gasiorek, J;Ruediger, E;Jolicouer, E;Marinier, A;Assouline, S;Borden, KLB; | Medicinal Chemistry and NMR Driven Discovery of Novel UDP-glucuronosyltransferase 1A Inhibitors That Overcome Therapeutic Resistance in Cells | Journal of molecular biology | 38043731 | Corning Human supersomes TM UGT1A1 (456411) and UGT1A4 (456414) were procured from Discovery Life Sciences. The extent of specific substrate glucuronide Save | Full Paper |
2023 | Ung, J;Tan, SF;Fox, TE;Shaw, JJP;Taori, M;Horton, BJ;Golla, U;Sharma, A;Szulc, ZM;Wang, HG;Chalfant, CE;Cabot, MC;Claxton, DF;Loughran, TP;Feith, DJ; | Acid Ceramidase Inhibitor LCL-805 Antagonizes Akt Signaling and Promotes Iron-Dependent Cell Death in Acute Myeloid Leukemia | Cancers | 38136410 | irginia School of Medicine and in accordance with the Declaration of Helsinki (Supplemental Table S1). Normal peripheral blood mononuclear cells (PBMCs) and bone marrow (BM) cells were purchased from AllCells (Alameda, CA, USA), Virginia Blood Services, Zen-Bio (Durham, NC, USA), and Inova Blood Donor Services (Sterling, VA, USA). PBMCs and BM mononuclear cells were isolated from patients’ whole | Full Paper |
2023 | Sheinin, M;Mondal, S;Pahan, K; | Neutralization of p40 Homodimer and p40 Monomer Leads to Tumor Regression in Patient-Derived Xenograft Mice with Pancreatic Cancer | Cancers | 38136341 | matched healthy controls were purchased from Discovery Life Sciences, Los Osos, CA, Serum samples were purchased mainly from Discovery Life Sciences (DLS). Some Save Cached;om Rush University Medical Center. Controls are defined as healthy individuals without significant medical history. Details are included below (see Table 2). Serum samples were purchased mainly from Discovery Life Sciences (DLS). Some normal samples were obtained from Rush University Medical Center (RUMC). Each sample was analyzed for p40, p402, and IL-12 three times by ELISA. 2.3. Sandwich ELIS | Full Paper |
2023 | Moreno, CS;Winham, CL;Alemozaffar, M;Klein, ER;Lawal, IO;Abiodun-Ojo, OA;Patil, D;Barwick, BG;Huang, Y;Schuster, DM;Sanda, MG;Osunkoya, AO; | Integrated Genomic Analysis of Primary Prostate Tumor Foci and Corresponding Lymph Node Metastases Identifies Mutations and Pathways Associated with Metastasis | Cancers | 38067373 | ) analysis was also performed by the EIGC on all RNA and DNA extractions and RNAseq and WES were performed by HudsonAlpha (subunit of Discovery Life Sciences). Save Cached;using the Covaris truXTRAC FFPE total NA kit. Quality control (QC) analysis was also performed by the EIGC on all RNA and DNA extractions and RNAseq and WES were performed by HudsonAlpha (subunit of Discovery Life Sciences). For RNAseq, we performed 100 bp paired-end sequencing using the SMART-Seq Stranded library prep kit (Takara, 634444). For WES analysis, libraries were prepared using the KAPA | Full Paper |
2023 | Melton, C;Freese, P;Zhou, Y;Shenoy, A;Bagaria, S;Chang, C;Kuo, C;Scott, E;Srinivasan, S;Cann, G;Roychowdhury-Saha, M;Chang, P;Singh, A; | A Novel Tissue-Free Method to Estimate Tumor-Derived Cell-Free DNA Quantity Using Tumor Methylation Patterns | Cancers | Additional tissue and cell type samples had been obtained commercially via Discovery Life Sciences, Huntsville, AL, USA (formerly Conversant Biologics, Inc.). Tissue Save Cached;- Simple Summary Tumor cells shed pieces of DNA that circulate in biofluids like blood and urine. The amount of circulating tumor DNA is related to the amount of cancer in Save Cached | Full Paper | |
2023 | Rice, M;Nuovo, GJ;Sawant, D;Mishra, A;Tili, E; | Comparison of Neuroinflammation Induced by Hyperphosphorylated Tau Protein Versus Ab42 in Alzheimer's Disease | Molecular neurobiology | 38105410 | The patient samples (formalin-fixed paraffin-embedded autopsy material) originated from the files of Folio Biosciences. Clinical information included the diagnosis, age/sex Save | Full Paper |
2023 | Jasani, B;Taniere, P;Schildhaus, HU;Blighe, K;Parry, S;Wilkinson, D;Atkey, N;Clare-Antony, S;McCabe, C;Quinn, C;CLDN Study Group, ;Dodson, A; | Global Ring Study to Investigate the Comparability of Total Assay Performance of Commercial Claudin 18 Antibodies for Evaluation in Gastric Cancer | Laboratory investigation; a journal of technical methods and pathology | 37949357 | Discovery Life Sciences (DLS) Biomarker Services GmbH, Kassel, Germany.++University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.++Discovery Life Sciences (DLS) Biomarker Services GmbH, Kassel, Germany.++Clinical Bioinformatics Research Ltd, London, UK.++UK National External Quality Assessment Scheme for Immunocytochemistry & In-Situ Hybridisation, London, UK.++UK National External Quality Assessment Scheme for Immunocytochemistry & In-Situ Hybridisation, London, UK.++Diaceutics PLC, Belfast, UK.++Diaceutics PLC, Belfast, UK.++Diaceutics PLC, Belfast, UK.++Diaceutics PLC, Belfast, UK. Electronic address: christine.quinn@diaceutics.com.++See the details of CLDN Study Group members in the acknowledgments.++UK National External Quality Assessment Scheme for Immunocytochemistry & In-Situ Hybridisation, London, UK | Full Paper |
2024 | Zhang, D;Zhou, G;Thongda, W;Li, C;Ye, Z;Zhao, H;Beck, BH;Mohammed, H;Peatman, E; | Early divergent responses to virulent and attenuated vaccine isolates of Flavobacterium covae sp. nov. In channel catfish, Ictalurus punctatus | Fish & shellfish immunology | 38030028 | The RNA-Seq library was constructed and sequenced by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were constructed with 2.14-3.25 ?g of Save | Full Paper |
2023 | Mairinger, E;Wessolly, M;Buderath, P;Borchert, S;Henrich, L;Mach, P;Steinborn, J;Kimming, R;Jasani, B;Schmid, KW;Bankfalvi, A;Mairinger, FD; | Tumor cell cytoplasmic metallothionein expression associates with differential tumor immunogenicity and prognostic outcome in high-grade serous ovarian carcinoma | Frontiers in oncology | 38023247 | Institute of Pathology, University Hospital Essen, Essen, Germany.++Department of Gynecology and Obstetrics, University Hospital Essen, Essen, Germany.++Department of Gynecology and Obstetrics, University Hospital Essen, Essen, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany.++Department of Gynecology and Obstetrics, University Hospital Essen, Essen, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany.++Department of Gynecology and Obstetrics, University Hospital Essen, Essen, Germany.++Department of Pathology, Targos - A Discovery Life Sciences Company, Kassel, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany | Full Paper |
2023 | Bonnevie, ED;Dobrzynski, E;Steiner, D;Hildebrand, D;Monslow, J;Singh, M;Decman, V;Krull, DL; | A machine learning approach toward automating spatial identification of LAG3+/CD3+ cells in ulcerative colitis | Scientific reports | 38066073 | Human biological samples were sourced ethically from commercial vendors (BioIVT/Asterand and Discovery Life Sciences) that have been approved by GSK human Save;e to better understand disease progression and treatment potential. Materials and methods Method overview Human biological samples were sourced ethically from commercial vendors (BioIVT/Asterand and Discovery Life Sciences) that have been approved by GSK human biological sample management ethical committee. Prior to sample acquisition informed consent for general research was obtained by commerci | Full Paper |
2023 | Gao, L;Kyubwa, EM;Starbird, MA;Diaz de Leon, J;Nguyen, M;Rogers, CJ;Menon, N; | Circulating miRNA profiles in COVID-19 patients and meta-analysis: implications for disease progression and prognosis | Scientific reports | 38065980 | effectiveness. Materials and methods Clinical specimen Adult patients hospitalized in Erie County, NY, with PCR-confirmed COVID-19 between March and November 2020 were retrospectively identified by Discovery Life Sciences (DLS; Huntsville, AL). Plasma samples were collected within the initial seven days of hospitalization. Demographic and clinical profiles, encompassing laboratory data, were ext | Full Paper |
2023 | Stecklein, SR;Barlow, W;Pusztai, L;Timms, K;Kennedy, R;Logan, GE;Seitz, R;Badve, S;Gökmen-Polar, Y;Porter, P;Linden, H;Tripathy, D;Hortobagyi, GN;Godwin, AK;Thompson, A;Hayes, DF;Sharma, P; | Dual Prognostic Classification of Triple-Negative Breast Cancer by DNA Damage Immune Response and Homologous Recombination Deficiency | JCO precision oncology | 37972336 | peakers’ Bureau: Genomic Health, Targos GmbH, Agilent Research Funding: Dako/Agilent Technologies (Inst), Lilly (Inst), Bristol Myers Squibb Foundation (Inst) Patents, Royalties, Other Intellectual Property: EarlyR—signature for ER1 breast cancer (Inst), E-Score for predicting recu | Full Paper |
2023 | Robertson, IB;Mulvaney, R;Dieckmann, N;Vantellini, A;Canestraro, M;Amicarella, F;O'Dwyer, R;Cole, DK;Harper, S;Dushek, O;Kirk, P; | Tuning the potency and selectivity of ImmTAC molecules by affinity modulation | Clinical and experimental immunology | 37930865 | T cell engaging bispecifics have great clinical potential for the treatment of cancer and infectious diseases. The binding affinity and kinetics of a bispecific molecule for both Save | Full Paper |
2023 | Scheel, AH;Lamberty, H;Tolkach, Y;Gebauer, F;Schoemig-Markiefka, B;Zander, T;Buettner, R;Rueschoff, J;Bruns, CJ;Schroeder, W;Quaas, A; | Tumour area infiltration and cell count in endoscopic biopsies of therapy-naive upper GI tract carcinomas by QuPath analysis: implications for predictive biomarker testing | Scientific reports | 37845307 | Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.++Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.++Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.++Department of General, Visceral and Cancer Surgery, University Hospital Cologne, Medical Faculty, University of Cologne, Cologne, Germany.++Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.++Department of Internal Medicine I, University Hospital Cologne, Medical Faculty, University of Cologne, Cologne, Germany.++Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.++Targos Molecular Pathology GmbH, Kassel, Germany.++Department of General, Visceral and Cancer Surgery, University Hospital Cologne, Medical Faculty, University of Cologne, Cologne, Germany.++Department of General, Visceral and Cancer Surgery, University Hospital Cologne, Medical Faculty, University of Cologne, Cologne, Germany.++Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany. alexander.quaas@uk-koeln.de | Full Paper |
2023 | Van den Ackerveken, P;Lobbens, A;Pamart, D;Kotronoulas, A;Rommelaere, G;Eccleston, M;Herzog, M; | Epigenetic profiles of elevated cell free circulating H3.1 nucleosomes as potential biomarkers for non-Hodgkin lymphoma | Scientific reports | 37770512 | by Discovery Life Sciences (Discovery Life Sciences, Huntsville, Texas, USA; protocol/study). For healthy donors (iNoSpecimens BioBank, Clermont Ferrand, France), the Save | Full Paper |
2023 | Pan, Y;Rohde, M;Zeitler, J;Namburi, SVS;Cao, L;Hu, J;Meyer, K;Lu, Y; | A sensitive AAV transduction inhibition assay assists evaluation of critical factors for detection and concordance of pre-existing antibodies | Molecular therapy. Methods & clinical development | 37920239 | g PBS and 0.001% Pluronic F-68. AAV copy concentration were determined by qPCR. Human serum samples Individual human serum samples from healthy donors were purchased from BioIVT (Westbury, NY, USA), Discovery Life Sciences (Los Osos, CA, USA), and Golden West Biologicals (Temecula, CA, USA). Donors varied in ethnicity (Black, White, Hispanic, and Asian), ages, and geographical locations in the Un | Full Paper |
2023 | Poletti, V;Montepeloso, A;Pellin, D;Biffi, A; | Prostaglandin E2 as transduction enhancer affects competitive engraftment of human hematopoietic stem and progenitor cells | Molecular therapy. Methods & clinical development | 37920236 | of a modification of our current transduction protocol with the addition of PGE2. Materials and methods Human cell culture and transduction Human CD34+ cells from healthy donors were purchased from AllCells. After thawing, cells were pre-activated for 24 h in GMP SCGM medium (CellGenix) added of a human recombinant cytokines cocktail (PeproTech, Inc.), as previously published.17 Three hundred t | Full Paper |
2023 | Lazarski, CA;Hanley, PJ; | Review of flow cytometry as a tool for cell and gene therapy | Cytotherapy | 37943204 | PJH is a co-founder and on the board of directors of Mana Therapeutics. He is or was an advisor or on the scientific advisory board of: Cellenkos, Cellevolve, Discovery Life Sciences, Capsida, Autolomous, MicrofluidX and March Biosciences. CAL has no disclosures | Full Paper |
2023 | Moore, JL;Charkoftaki, G; | A Guide to MALDI Imaging Mass Spectrometry for Tissues | Journal of proteome research | 37877579 | Department of Proteomics, Discovery Life Sciences, Huntsville, Alabama 35806, United States.++Department of Environmental Health Sciences, Yale School of Public Health, Yale University, New Haven, Connecticut 06520, United States | Full Paper |
2024 | O'Brien, G;Cecotka, A;Manola, KN;Pagoni, MN;Polanska, J;Badie, C; | Epigenetic signature of ionizing radiation in therapy-related AML patients | Heliyon | 38163095 | Research (NICR), Newcastle REC (17/NE/0361). Bone marrow aspirate samples were also obtained from 5 healthy donors from AllCells® (Alamada, USA) | Full Paper |
2023 | Hemken, PM;Qin, X;Sokoll, LJ;Jackson, L;Feng, F;Li, P;Gawel, SH;Tu, B;Lin, Z;Hartnett, J;Hawksworth, D;Tieman, BC;Yoshimura, T;Kinukawa, H;Ning, S;Liu, E;Meng, F;Chen, F;Miao, J;Mi, X;Tong, X;Chan, DW;Davis, GJ; | Validation of the novel GLAS algorithm as an aid in the detection of liver fibrosis and cirrhosis based on GP73, LG2m, age, and sex | Clinical proteomics | 38017436 | iver disease. An additional set of serum samples (collectively referred to as the Western Vendor Cohort, WVC; n?=?246) were purchased from BioIVT (Wesbury, NY), Biomex GmbH (Heidelberg, Germany), Discovery Life Sciences (Huntsville, AL), and ProMedDx (Norton, MA). These three sample sets (JHU/UTSMC/WVC) were combined to develop and train the liver fibrosis and cirrhosis diagnostic algorithm (d | Full Paper |
2023 | Chen, S;Li, X;Li, Y;He, X;Bryant, M;Qin, X;Li, F;Seo, JE;Guo, X;Mei, N;Guo, L; | The involvement of hepatic cytochrome P450s in the cytotoxicity of lapatinib | Toxicological sciences : an official journal of the Society of Toxicology | 37788138 | were purchased from Discovery Life Sciences (Columbia (UCRM-A, Discovery Life Sciences) and seeded in 384- -A (UPCM-A, Discovery Life Sciences) for 4 h prior to the Save | Full Paper |
2023 | LeCleir, GR;Bassett, J;Wilhelm, SW; | Effects of iron concentration and DFB (Desferrioxamine-B) on transcriptional profiles of an ecologically relevant marine bacterium | PloS one | 38100448 | les were subjected to a subsequent round of DNAse treatment. All samples were verified to be free from genomic DNA within two rounds of DNAse treatment. The Hudson Alpha Genome Sequencing Center (now Discovery Life Sciences; https://www.hudsonalpha.org/gsc/) performed rRNA reduction and mRNA transcript library sequencing using a 2 x 100 nucleotide protocol on the Illumina Novaseq platform. Nucleot | Full Paper |
2023 | Shyu, JY;Schlag, PA;Karwowska, SM;Manohar, CF;Truong, HM;Longshore, JW;Zhang, G; | Performance of the cobas EZH2 mutation test on clinical samples from non-Hodgkin lymphoma patients | PloS one | 38096164 | Objective To present the technical verification and clinical validation of the companion diagnostic assay, cobas EZH2 Mutation Test (cobas EZH2 Test), targeting gain-of- Save Cached;ous mutant variants of EZH2 were acquired from 14 vendors (Analytical Biological Services, Inc.; Asterand, Inc.; Aurora Diagnostics, Inc.; Bio-Options, Inc.; BioServe, Inc.; Cambridge Biosource, LLC; Conversant Bio; Folio Biosciences; ILSbio, LLC; Indivumed GmbH; MT Group; PhenoPath, PLLC; ProteoGenex, Inc.; Tissue Source, LLC). These were de-identified remnant FFPE tumor tissue samples from biops | Full Paper |
2023 | Karbhari, A;Mosessian, S;Trivedi, KH;Valla, F;Jacobson, M;Truty, MJ;Patnam, NG;Simeone, DM;Zan, E;Brennan, T;Chen, H;Kuo, PH;Herrmann, K;Goenka, AH; | Gallium-68-labeled fibroblast activation protein inhibitor-46 PET in patients with resectable or borderline resectable pancreatic ductal adenocarcinoma: A phase 2, multicenter, single arm, open label non-randomized study protocol | PloS one | 38011131 | sections from biopsied or resected specimens will be evaluated by standard histopathologic methods at an independent centralized core facility (Discovery Life Sciences, Save Cached;Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of America.++Clinical Development, Sofie Biosciences, Dulles, Virginia, United States of America.++Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of America.++Radiopharmaceutical and Contract Manufacturing, Sofie Biosciences, Dulles, Virginia, United States of America.++Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of America.++Department of Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.++Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of America.++Departments of Surgery and Pathology, NYU Langone Health, New York, New York, United States of America.++Department of Radiology, Weill Cornell Medicine, New York, New York, United States of America.++Discovery Life Sciences, Newtown, Pennsylvania, United States of America.++Discovery Life Sciences, Newtown, Pennsylvania, United States of America.++Departments of Medical Imaging, Medicine and Biomedical Engineering, University of Arizona, Tucson, Arizona, United States of America.++Department of Nuclear Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.++Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of America | Full Paper |
2023 | Zahavi, DJ;Erbe, R;Zhang, YW;Guo, T;Malchiodi, ZX;Maynard, R;Lekan, A;Gallagher, R;Wulfkuhle, J;Petricoin, E;Jablonski, SA;Fertig, EJ;Weiner, LM; | Antibody dependent cell-mediated cytotoxicity selection pressure induces diverse mechanisms of resistance | Cancer biology & therapy | 37878417 | Fresh healthy donor NK cells from five different donors were purchased from AllCells with CD56 negative selection (PB012-N). Donor NK cells were expanded and Save | Full Paper |
2023 | Mohebbi Najm Abad, J;Farahbakhsh, A;Mir, M;Alizadeh, R;Hekmatmanesh, A; | Urea-Self Powered Biosensors: A Predictive Evolutionary Model for Human Energy Harvesting | Sensors (Basel, Switzerland) | 37837010 | modified Pt electrode Turk. J. Chem. 2019 43 1063 1074 10.3906/kim-1901-13 21. Ramezani AzghandiO. FarahbakhshA. The obtain optimum production conditions for Glucose Oxidase biosensor using software Qualtek-4 Int. J. Nano Dimens. (IJND) 2015 6 23 30 10.7508/IJND.2015.06.004 22. YangW. HanW. GaoH. ZhangL. WangS. XingL. ZhangY. XueX. Self-powered implantable electronic-skin for in situ analysis of | Full Paper |
2023 | Baze, A;Wiss, L;Horbal, L;Biemel, K;Asselin, L;Richert, L; | Comparison of in vitro thyroxine (T4) metabolism between Wistar rat and human hepatocyte cultures | Toxicology in vitro : an international journal published in association with BIBRA | 38142784 | ts and media used are listed as follows. Universal Cryopreservation Recovery Medium (UCRM™) and Universal Primary Cells Plating Medium (UPCM™) were purchased from Discovery Life Sciences (DLS, Columbia, MD). INVITROGRO CP Rodent Medium and Torpedo Rodent Antibiotic Mix were purchased from BioIVT (Baltimore, Maryland, US), Matrigel® and Collagen I Rat Tail from Corning (Avon, France) and William's medium E (WE)?+?GLUTAMAX, Insulin-Transferrin-Selenium (ITS), phosphate-buffered saline (PBS) 1×, Dulbecco's Modified Eagle Medium (DMEM), MagMAX mirVana Total RNA Isolation Kit and Penicillin/ | Full Paper |
2023 | Ali, KA;Shah, RD;Dhar, A;Myers, NM;Nguyen, C;Paul, A;Mancuso, JE;Scott Patterson, A;Brody, JP;Heiser, D; | Ex vivo discovery of synergistic drug combinations for hematologic malignancies | SLAS discovery : advancing life sciences R & D | 38101570 | Bone marrow samples from healthy volunteers were supplied by AllCells, Inc. (Alameda, CA). This study was reviewed by the WCG Institutional Review Board and Save | Full Paper |
2023 | Ferri, N;De Martin, S;Stuart, J;Traversa, S;Folli, F;Pappagallo, M;O'Gorman, C;Guidetti, C;Mattarei, A;Inturrisi, CE;Manfredi, PL; | Drug-Drug Interaction Studies of Esmethadone (REL-1017) Involving CYP3A4- and CYP2D6-Mediated Metabolism | Drugs in R&D | 38010591 | Background and Objective Esmethadone (dextromethadone; d-methadone; S-methadone (+)-methadone; REL-1017) is the opioid inactive dextro-isomer of racemic Save;- Thawing medium (Universal Cryo Recovery Medium) for hepatocytes was purchased from In Vitro ADMET Laboratories (iVAL, Columbia, MD, USA). Hepatocyte culture Save | Full Paper |
2023 | Vertacnik, KL;Herrig, DK;Godfrey, RK;Hill, T;Geib, SM;Unckless, RL;Nelson, DR;Linnen, CR; | Evolution of five environmentally responsive gene families in a pine-feeding sawfly, Neodiprion lecontei (Hymenoptera: Diprionidae) | Ecology and evolution | 37791292 | sessed with 0.7% agarose gel, purity was measured with the 260/280 ratio, and concentration was measured with a Quant?iT dsDNA High?Sensitivity fluorescence assay (Thermo Fisher Scientific). The HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) prepared and sequenced the DNA libraries. Two small?insert, barcoded libraries with average fragment sizes of 337?bp and 864?bp were made f | Full Paper |
2023 | Chalmers, SB;van der Wal, T;Fre, S;Jonkers, J; | Fourteenth Annual ENBDC Workshop: Methods in Mammary Gland Biology and Breast Cancer | Journal of mammary gland biology and neoplasia | 37801168 | Sandra Schoniger (Discovery Life Sciences Biomarker Services GmbH, Kassel, Germany) gave the final presentation of the session, focussed on the characterisation of Save;l hotspots of Ki67 colocalised with immune cell aggregates that resembled tertiary lymphoid structures, a hitherto unrecognised component of the ovine mammary gland immune system. Sandra Schöniger (Discovery Life Sciences Biomarker Services GmbH, Kassel, Germany) gave the final presentation of the session, focussed on the characterisation of mammary tumours in pet rabbits. Rabbits may represent | Full Paper |
2023 | Koc, MA;Wiles, TA;Weinhold, DC;Rightmyer, S;Weaver, AL;McDowell, CT;Roder, J;Asmellash, S;Pestano, GA;Roder, H;Georgantas Iii, RW; | Molecular and translational biology of the blood-based VeriStrat® proteomic test used in cancer immunotherapy treatment guidance | Journal of mass spectrometry and advances in the clinical lab | 38074293 | LC immunotherapy. 2 Material and methods 2.1 Samples De-identified, serum samples from human subjects diagnosed with lung cancer were acquired from commercial biobanks under their consent protocols [Discovery Life Sciences (Huntsville, AL), ProMedDx (Norton, MA), ProteoGenex (Inglewood, CA) and BioIVT (Westbury, NY)]. Additional archived, de-identified samples were from patients consented into th | Full Paper |
2023 | Okada, K;Yokota, J;Yamashita, T;Inui, T;Kishimoto, W;Nakase, H;Mizuguchi, H; | Establishment of human intestinal organoids derived from commercially available cryopreserved intestinal epithelium and evaluation for pharmacokinetic study | Drug metabolism and pharmacokinetics | 38064926 | Following the manufacturer's instructions, cryopreserved human intestinal epithelial cells (Cryopreserved Human Enterocytes, Discovery Life Science, AL, USA) were thawed by incubation in a 37 °C water bath for 90 s. The cells were then centrifuged at 100 g for 10 min to create a pellet. The supernatant was removed and suspended in Matrigel. The pellet was resuspended in Matrigel, and a 40 ?L aliquot of the resulting cell suspension was added to the center of each well of a 24-well plate. The Matrigel was polymerized for 10 | Full Paper |
2023 | Shaikh, N;Zhang, X;Abdalla, M;Baylink, D;Tang, X; | Enhancing Human Treg Cell Induction through Engineered Dendritic Cells and Zinc Supplementation | Critical Reviews in Immunology | These 102 PBMCs were isolated from a Leukopak obtained from AllCells, LLC. The study was reviewed by 103 the Long Island University Institutional Review Board (IRB) Save | Full Paper | |
2023 | Rüschoff, J;Schildhaus, HU;Rüschoff, JH;Jöhrens, K;Bocker-Edmonston, T;Dietmaier, W;Bläker, H;Baretton, G;Horst, D;Dietel, M;Hartmann, A;Klauschen, F;Merkelbach-Bruse, S;Stenzinger, A;Schöniger, S;Tiemann, M;Weichert, W;Büttner, R; | [Not Available] | Pathologie (Heidelberg, Germany) | 37656216 | Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland. josef.rueschoff@dls.com.++Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland.++Institut für Pathologie und Molekularpathologie, Universitätsspital Zürich, Zürich, Schweiz.++Institut für Pathologie, Universitätsklinikum Carl Gustav Carus Dresden, Dresden, Deutschland.++Department of Pathology, Cooper University Health Care, Camden, NJ, USA.++Institut für Pathologie/Zentrum für molekularpathologische Diagnostik, Universität Regensburg, Regensburg, Deutschland.++Institut für Pathologie, Universitätsklinikum Leipzig, Leipzig, Deutschland.++Institut für Pathologie, Universitätsklinikum Carl Gustav Carus Dresden, Dresden, Deutschland.++Institut für Pathologie, Charité - Universitätsmedizin Berlin, Campus Mitte, Berlin, Deutschland.++Institut für Pathologie, Charité - Universitätsmedizin Berlin, Campus Mitte, Berlin, Deutschland.++Pathologisches Institut, Universität Erlangen-Nürnberg, Erlangen, Deutschland.++Pathologisches Institut, Ludwig-Maximilians-Universität München, München, Deutschland.++Institut für Pathologie, Universitätsklinikum Köln, Köln, Deutschland.++Pathologisches Institut, Universitätsklinikum Heidelberg, Heidelberg, Deutschland.++Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland.++Institut für Hämatopathologie Hamburg, Hamburg, Deutschland.++Institut für Pathologie, Technische Universität München, München, Deutschland.++Institut für Pathologie, Universitätsklinikum Köln, Köln, Deutschland | Full Paper |
2023 | Abidin, MR;Alpan, O;Plassmeyer, M;Kozhaya, L;Loizou, D;Dogan, M;Upchurch, Z;Manes, NP;Nita-Lazar, A;Unutmaz, D;Sønder, SU; | STAT4 Phosphorylation of T-helper Cells predicts surgical outcomes in Refractory Chronic Rhinosinusitis | medRxiv : the preprint server for health sciences | 38168423 | Healthy adult blood was obtained from AllCells. Primary CD4 T cells were isolated, activated, 183 transduced to overexpress IL12Rb1 wild-type or mutant, and proliferated Save | Full Paper |
2023 | Landberg, N;Köhnke, T;Feng, Y;Nakauchi, Y;Fan, AC;Linde, MH;Karigane, D;Lim, K;Sinha, R;Malcovati, L;Thomas, D;Majeti, R; | IDH1-mutant preleukemic hematopoietic stem cells can be eliminated by inhibition of oxidative phosphorylation | Blood cancer discovery | 38091010 | Healthy adult peripheral blood-derived CD34+ 458 HSPCs were purchased from AllCells. Mononuclear cells from AML patients were isolated 459 from peripheral blood or Save | Full Paper |
2023 | Rumker, L;Sakaue, S;Reshef, Y;Kang, JB;Yazar, S;Alquicira-Hernandez, J;Valencia, C;Lagattuta, KA;Mah-Som, A;Nathan, A;Powell, JE;Loh, PR;Raychaudhuri, S; | Identifying genetic variants that influence the abundance of cell states in single-cell data | bioRxiv : the preprint server for biology | 38014313 | y the resulting simulated SNP decays. We generated 94 × 10 × 100 = 94,000 total simulates. We ran GeNA to conduct one csaQTL survey for associations per single-cell data object (T, B, NK, myeloid, allcells) using as input all SNPs defined to associate with true phenotypes within that data object. We ran GeNA with default parameters and controlled for age, sex, batch and gPC1-6 to generate an | Full Paper |
2023 | Filosto, S;Vardhanabhuti, S;Canales, MA;Poire, X;Lekakis, LJ;de Vos, S;Portell, CA;Wang, Z;To, C;Schupp, M;Poddar, S;Trinh, T;Warren, CM;Aguilar, EG;Budka, J;Cheng, P;Chou, J;Bot, A;Shen, RR;Westin, JR; | Product attributes of CAR T-cell therapy differentially associate with efficacy and toxicity in second-line large B-cell lymphoma (ZUMA-7) | Blood cancer discovery | 37983485 | CAR T-cell products were generated from apheresis material from healthy 478 donors (AllCells) following the axi-cel manufacturing protocol. Anti-CD19 CAR T effector Save | Full Paper |
2023 | Ho, C;Gopal, AK;Till, BG;Shadman, M;Lynch, RC;Cowan, AJ;Wu, QV;Voutsinas, J;Rasmussen, HA;Blue, K;Ujjani, CS;Cassaday, RD;Fromm, JR;Fang, M;Smith, SD; | Pembrolizumab With R-CHOP in Previously Untreated DLBCL: Sustained, High Efficacy, and Safety With Long-Term Follow-Up | Clinical lymphoma, myeloma & leukemia | 37926672 | PD-L1 expression was evaluated as an exploratory endpoint, and PD-L1 immunohistochemistry (IHC) on available tissue samples was performed by QualTek Molecular Save | Full Paper |
2023 | Tierney, BT;Kim, J;Overbey, EG;Ryon, KA;Foox, J;Sierra, M;Bhattacharya, C;Damle, N;Najjar, D;Park, J;Garcia Medina, S;Houerbi, N;Meydan, C;Wain Hershberg, J;Qiu, J;Kleinman, A;Al Ghalith, G;MacKay, M;Afshin, EE;Dhir, R;Borg, J;Gatt, C;Brereton, N;Readhead, B;Beyaz, S;Venkateswaran, KJ;Blease, K;Moreno, J;Boddicker, A;Zhao, J;Lajoie, B;Scott, RT;Altomare, A;Kruglyak, S;Levy, S;Church, G;Mason, CE; | Viral activation and ecological restructuring characterize a microbiome axis of spaceflight-associated immune activation | Research square | 37886447 | For metatranscriptomic sequencing, library preparation and sequencing were performed at 746 Discovery Life Sciences (Huntsville, Alabama). The extracted RNA went Save;sequenced on a S4 flow cell of the Illumina NovaSeq 6000 Sequencing System with 2 × 150 bp paired-end reads. For metatranscriptomic sequencing, library preparation and sequencing were performed at Discovery Life Sciences (Huntsville, Alabama). The extracted RNA went through an initial purification and cleanup with DNase digestion using the Zymo Research RNA Clean & Concentrator Magbead Kit (cat | Full Paper |
2023 | Frigault, MM;Mithal, A;Wong, H;Stelte-Ludwig, B;Mandava, V;Huang, X;Birkett, J;Johnson, AJ;Izumi, R;Hamdy, A; | Enitociclib, a Selective CDK9 Inhibitor, Induces Complete Regression of MYC+ Lymphoma by Downregulation of RNA Polymerase II Mediated Transcription | Cancer research communications | 37882668 | Differential gene expression analysis was performed (Fios Genomics) from RNA-seq data (Discovery Life Sciences). MYC and MCL1 mRNA levels were measured by Save;ed total RNA was evaluated using an Agilent 2100 Bioanalyzer system and RNA 6000 Nano Kit (Agilent, #5067-1511). Differential gene expression analysis was performed (Fios Genomics) from RNA-seq data (Discovery Life Sciences). MYC and MCL1 mRNA levels were measured by qPCR in vitro and in vivo, using a Taqman qPCR system (Thermo Fisher Scientific), TaqMan Fast Universal PCR Master Mix (2X) no AmpEr | Full Paper |
2023 | Jin, L;Wang, F;Wang, X;Harvey, BP;Bi, Y;Hu, C;Cui, B;Darcy, AT;Maull, JW;Phillips, BR;Kim, Y;Jenkins, GJ;Sornasse, TR;Tian, Y; | Identification of Plasma Biomarkers from Rheumatoid Arthritis Patients Using an Optimized Sequential Window Acquisition of All THeoretical Mass Spectra (SWATH) Proteomics Workflow | Proteomes | 37873874 | with methotrexate for at least three months. Only baseline plasma samples (K2-EDTA blood collection) were selected for this experiment. EDTA plasma samples from healthy volunteers were obtained from Conversant Biologics, Inc., Huntsville, Alabama, USA. 2.2. Sample Fractionation and Spectral Library Generation 2.2.1. Sample Preparation To prepare the experiment-specific plasma peptide spectrum li ... with methotrexate for at least three months. Only baseline plasma samples (K2-EDTA blood collection) were selected for this experiment. EDTA plasma samples from healthy volunteers were obtained from Conversant Biologics, Inc., Huntsville, Alabama, USA. 2.2. Sample Fractionation and Spectral Library Generation 2.2.1. Sample Preparation To prepare the experiment-specific plasma peptide spectrum li | Full Paper |
2023 | Hinestrosa, JP;Sears, RC;Dhani, H;Lewis, JM;Schroeder, G;Balcer, HI;Keith, D;Sheppard, BC;Kurzrock, R;Billings, PR; | Development of a blood-based extracellular vesicle classifier for detection of early-stage pancreatic ductal adenocarcinoma | Communications medicine | 37857666 | against this deadly disease. Methods Specimen collection The specimens for the training set were obtained from commercial biorepositories (ProteoGenex, Culver City, CA; DxBiosamples, San Diego, CA; Discovery Life Sciences, Huntsville, AL; Fox Chase Cancer Center, Philadelphia, PA; iSpecimen, Lexington MA; Tissue for Research, Newmarket, England). The specimens for the validation set were obtaine | Full Paper |
2023 | Adema, V;Ganan-Gomez, I;Ma, F;Rodriguez-Sevilla, JJ;Chien, K;Yang, H;Thongon, N;Kanagal-Shamanna, R;Loghavi, S;Montalban-Bravo, G;Hammond, D;Gu, Y;Tan, R;Tan, L;Lorenzi, P;Al-Atrash, G;Clise-Dwyer, K;Bejar, R;Pellegrini, M;Garcia-Manero, G;Colla, S; | IL-1?-mediated inflammatory signaling drives ineffective erythropoiesis in early-stage myelodysplastic syndromes | bioRxiv : the preprint server for biology | 37808770 | Myelodysplastic syndromes (MDS) are a group of incurable hematopoietic stem cell (HSC) neoplasms characterized by peripheral blood cytopenias and a high risk of Save | Full Paper |
2023 | Prakash, S;Bharti, S;Priyatma, AR; | Performance Characteristic of a Simple, Rapid and Cost-Effective Micro-PCR Assay for HBV DNA Quantification | J Med Diagn Meth | The genotype panel was obtained from Discovery Life Sciences (DLS). After duplicate extraction of the corresponding genotype DNA using TruePrep Auto, PCR was Save | Full Paper | |
2023 | Gang, L;Li, H;Ge, P;Yu, J; | Whether AI+CDK4/6i+HP is suitable treatment for three positive metastatic breast cancer patient with renal insufficiency caused by type 2 diabetes | Research Square | Background: Hormone-receptor positive (HR+), ERBB2 gene mutation (HER2+) breast cancer is a very common type of breast cancer, but it is relatively rare for metastatic Save | Full Paper | |
2023 | Weiner, L;Fitzgerald, A;Maynard, R;Marcisak, E;Nasir, A;Glasgow, E;Jablonski, S;Veken, P;Pearson, G;Eisman, S;Mace, E;Fertig, E; | Fibroblast activation protein regulates natural killer cell migration, extravasation and tumor infiltration | Research Square | Fresh healthy donor NK cells were purchased from AllCells with either CD56 positive selection or CD56 negative selection (Allcells, cat#PB012-P or PB012-N). For 2D Save | Full Paper | |
2023 | Chen, C;Hamilton, E;LoRusso, P;Rottey, S;Papadopoulos, K;Kummar, S;Kotecki, N;Thistlethwaite, F;LeBruchec, Y;Dyczkowski, J;Rüschoff, J;Samari, S;Toukam, M;Boni, J;Havenith, K;Pantano, S;Puzanov, I; | 608?Results of a phase 1 study investigating camidanlumab tesirine as monotherapy and in combination with pembrolizumab in patients with selected advanced solid tumors | Regular and Young Investigator Award Abstracts | USA * 7Institut Jules Bordet, Brussels, Belgium * 8The Christie NHS Foundation Trust and University of Manchester, Manchester, UK * 9ADC Therapeutics SA, Épalinges, Switzerland * 10Targos - A Discovery Life Sciences Company, Kassel, Germany * 11ADC Therapeutics (UK) Ltd, London, UK * 12ADC Therapeutics America, Inc, Murray Hill, NJ, USA * 13Roswell Park Comprehensive Cancer Center, Buff | Full Paper | |
2023 | Melocchi, A;Schmittlein, B;Jones, A;Ainane, Y;Rizvi, A;Chan, D;Dickey, E;Pool, K;Harsono, K;Szymkiewicz, D;Scarfogliero, U;Bhatia, V;Sivanantham, A;Kreciglowa, N;Hunter, A;Gomez, M;Tanner, A;Uboldi, M;Batish, A;Balcerek, J;Kutova-Stoilova, M;Paruthiyil, S;Acevedo, L;Stadnitskiy, R;Carmichael, S;Aulbach, H;Hewitt, M;De Mollerat Du Jeu, X;Robilant, B;Parietti, F;Esensten, J; | Development of a robotic cluster for automated and scalable cell therapy manufacturing | bioRxiv | Human CD8+ T cells isolated from commercially available human leukopheresis collections (StemExpress, US-CA or AllCells, US-CA) using Miltenyi CD8+ microbeads and a Miltenyi Prodigy instrument were used for run 1. Purified human CD8+ T cells were purchased from a commercial source IQ Biosciences and used for runs 2 and 3 (US-CA) | Full Paper | |
2023 | Faccioli, L;Sun, Y;Motomura, T;Liu, Z;Kurihara, T;Hu, Z;Cetin, Z;Franks, J;Stolz, D;Ostrowska, A;Florentino, R;Fox, I;Soto-Gutierrez, A; | Human Induced Pluripotent Stem Cell based Hepatic-Modeling of Lipid metabolism associated TM6SF2 E167K variant | bioRxiv | BACKGROUND AND AIMS: TM6SF2 rs58542926 (E167K) is associated with an increase in the prevalence of Metabolic Disfunction-Associated Steatotic Liver Disease ( Save;- Cryopreserved primary hepatocytes from healthy individuals were obtained from In Vitro ADMET Laboratories Inc. (IVAL, Columbia, MD, USA). End-stage liver disease ( Save | Full Paper | |
2023 | Perez-Bermejo, J;Efagene, O;Matern, W;Holden, J;Kabir, S;Chew, G;Andreoletti, G;Catton, E;Ennis, C;Garcia, A;Gerstenberg, T;Hill, K;Jain, A;Krassovsky, K;Lalisan, C;Lord, D;Quejarro, B;Sales-Lee, J;Shah, M;Silva, B;Skowronski, J;Strukov, Y;Thomas, J;Veraz, M;Vijay, T;Wallace, K;Yuan, Y;Grogan, J;Wienert, B;Lahiri, P;Treusch, S;Dever, D;Soros, V;Partridge, J;Seim, K; | Functional Screening in human HSPCs identifies optimized protein-based enhancers of Homology Directed Repair | bioRxiv | CD34+ HSPCs were purchased from 544 AllCells and were isolated from G-CSF-mobilized peripheral blood from healthy donors. CD34+ HSPCs 545 were cultured at 2.5× Save | Full Paper | |
2023 | Chen, W;Yan, Y;Young, B;Pinto, A;Jiang, Q;Song, N;Yao, W;Zhang, D;Zhang, J; | Microsatellite Instability Detection in Clinical Cancer Samples: A Multiplex qPCR Approach without Matching Normal Samples | medRxiv | 48 samples with MSI status confirmed by PCR were purchased from 94 Discovery Life Sciences. All clinical samples information is collected from vendors and 95 Save | Full Paper | |
2023 | Cieniewicz, B;Oliveira, E;Saxton, M;Torabi, D;Bhatta, A;Kukutla, P;Arballo, A;Yang, Z;Yu, B;Fate, M;Ning, H;Corey, L;Maiti, A;Corey, D; | Therapeutic Targeting of TIM-4-L With Engineered T Cells for Acute Myeloid Leukemia | bioRxiv | Patient-derived AML bone marrow and PBMC samples and healthy donor controls were obtained from a contract research organization (Discovery Life Sciences). Save | Full Paper | |
2023 | Kocas-Kilicarslan, Z;Cetin, Z;Faccioli, L;Motomura, T;Amirneni, S;Diaz-Aragon, R;Florentino, R;Sun, Y;Pla-Palacin, I;Xia, M;Miedel, M;Kurihara, T;Hu, Z;Ostrowska, A;Wang, Z;Constantine, R;Li, A;Taylor, D;Behari, J;Soto-Gutierrez, A;Tafaleng, E; | Polymorphisms Associated With Metabolic Dysfunction-Associated Steatotic Liver Disease Influence the Progression of End-Stage Liver Disease | Gastro Hep Advances | Cryopreserved primary hepatocytes from 123 healthy individuals were obtained from In Vitro ADMET Laboratories Inc (IVAL, Columbia, MD). These hepatocytes were isolated from liver specimens of donors who were negative for Hepatitis C virus, Hepatitis B virus, and human immunodeficiency virus. Vials of cryopreserved cells were | Full Paper | |
2023 | Imai, M;Nakamura, Y;Denda, T;Komatsu, Y;Yuki, S;Nishina, T;Hamamoto, Y;Hara, H;Esaki, T;Kawakami, H;Kato, K;Satoh, T;Okano, N;Sunakawa, Y;Taniguchi, H;Yamaguchi, K;Yamada, T;Miki, I;Wakabayashi, M;Kuwata, T;Shitara, K;Yoshino, T; | Association of PD-L1 and PD-L2 expression and tumor-infiltrating lymphocytes in BRAF V600E-mutated metastatic colorectal cancer: GI-SCREEN post-hoc analysis | ESMO Gastrointestinal Oncology | PD-L1 and PD-L2 expression was assessed by the pathologists at Qualtek Laboratories and NeoGenomics Laboratories, respectively, in a blinded manner, for baseline Save | Full Paper | |
2023 | Al-Batran, S;Roetzer, I;Wohn, L;Blau, W;Zander, T;Luley, K;Ivanyi, P;Hofheinz, R;Groschek, M;Kreiss-Sender, J;Baur, C;Wolff, T;Wenning, B;Decker-Baumann, C;Bouymayan, S;Sookthai, D;Schaaf, M;Riedel, J;Goetze, T;Haag, G; | Home parenteral nutrition (HPN) using individually compounded PN via A 7/8/9-Multi-chamber system versus PN via 2/3-chamber bags in subjects with metastatic or localized solid tumors requiring HPN - The IKF-010 Pekannuss trial | Clinical Nutrition ESPEN | ier, MSD Oncology, Lilly, Bristol-Myers Squibb, Targos GmbH, iOMEDICO, MCI conventions, Taiho Pharmaceutical, Nordic Group, Consultant for: Bristol-Myers Squibb, MSD Oncology, Lilly, Novartis, Daiichi Sankyo Europe GmbH, Servier, Other: Lilly, Bristo | Full Paper | |
2023 | Sweeney, E;Sekhri, P;Muniraj, N;Chen, J;Feng, S;Terao, J;Chin, S;Schmidt, D;Bollard, C;Cruz, C;Fernandes, R; | Photothermal Prussian blue nanoparticles generate potent multi?targeted tumor?specific T cells as an adoptive cell therapy | Bioengineering & Translational Medicine | Healthy donor peripheral blood leukopaks were purchased from AllCells (Alameda, CA) with the corresponding HLA reports. Peripheral blood mononuclear cells (PBMCs) Save | Full Paper | |
2023 | Mukhamedshin, A;Reddington, R;Dinh, M;Abhishek, K;Iqbal, M;Manheim, M;Gifford, S;Shevkoplyas, S; | Rapid, label?free enrichment of lymphocytes in a closed system using a flow?through microfluidic device | Bioengineering & Translational Medicine | An additional three units from AllCells were picked up locally and processed on the day of collection. All leukapheresis samples were passed through a 40 ?m aggregate Save | Full Paper | |
2023 | Leyva Calero, A; | Desarrollo y evaluación de una técnica de quimioluminiscencia para la detección de antígeno de galactomanano de Aspergillus spp. en muestras biológicas. | Thesis | R-409-040 LBA Discovery Life Sciences | Full Paper | |
2023 | Santagata, S;Cahill, D;Curry, W;Barker, F;Dunn, IF; | DF/HCC Protocol#: 17-296 TITLE: Phase II Trial of Pembrolizumab in Recurrent or Residual High Grade Meningioma | classic.clinicaltrials.gov | PD-L1 expression will be measured by the QualTek assay as well as with RNA-Seq as specified below in 9.3.3. Save | Full Paper | |
2023 | Rogers, BB; | Cis-Regulatory Elements: Relevance for Alzheimer's Disease | Thesis | from each sample and the entire pool was processed according to the multiome protocol. Libraries were sequenced by HudsonAlpha Discovery using Illumina NovaSeq S4 flowcells | Full Paper | |
2023 | Kim, Y; | Systems Approach to Unravel Lymphoid Development in Human Bone Marrow | Thesis | Deidentified human bone marrow (BM) (n=9) were obtained from healthy adult donors (AllCells, Alameda, CA and StemExpress, Folsom, CA). Ex vivo labeling human bone marrow for CyTOF screen Fresh BM aspirates were labeled for their biosynthesis as previo | Full Paper | |
2023 | Alluhayb, AH; | COMPREHENSIVE ANALYSIS AND FORENSIC INVESTIGATION OF CYANIDE, THIOCYANATE, AND 2-AMINO-2-THIAZOLINE-4-CARBOXYLIC ACID INANTE- AND POSTMORTEM BLOOD UTILIZING ADVANCED ANALYTICAL | Thesis | irus (HCV), and Human Immunodeficiency virus (HIV). The donors had no known use of tobacco and no known history of alcohol abuse. The blood was collected by Discovery Life Sciences (Huntsville, Alabama, United States) and shipped on dry ice to the Department of Chemistry and Biochemistry at South Dakota State University. Upon receipt, all blood samples were stored at -80 °C until thawed for method development and validation. Method development of the analysis technique for whole (antemortem) blood utilized human whole blood drawn from a healthy individual caucasian ma | Full Paper | |
2024 | Ross, JB;Myers, LM;Noh, JJ;Collins, MM;Carmody, AB;Messer, RJ;Dhuey, E;Hasenkrug, KJ;Weissman, IL; | Depleting myeloid-biased haematopoietic stem cells rejuvenates aged immunity | Nature | 38538791 | For human HSC83 flow cytometry analysis, bone marrow mononuclear cells from young-adult donors (aged 26-33 years) were commercially obtained (AllCells). C | Full Paper |
2024 | George, J;Maas, L;Abedpour, N;Cartolano, M;Kaiser, L;Fischer, RN;Scheel, AH;Weber, JP;Hellmich, M;Bosco, G;Volz, C;Mueller, C;Dahmen, I;John, F;Alves, CP;Werr, L;Panse, JP;Kirschner, M;Engel-Riedel, W;Jürgens, J;Stoelben, E;Brockmann, M;Grau, S;Sebastian, M;Stratmann, JA;Kern, J;Hummel, HD;Hegedüs, B;Schuler, M;Plönes, T;Aigner, C;Elter, T;Toepelt, K;Ko, YD;Kurz, S;Grohé, C;Serke, M;Höpker, K;Hagmeyer, L;Doerr, F;Hekmath, K;Strapatsas, J;Kambartel, KO;Chakupurakal, G;Busch, A;Bauernfeind, FG;Griesinger, F;Luers, A;Dirks, W;Wiewrodt, R;Luecke, A;Rodermann, E;Diel, A;Hagen, V;Severin, K;Ullrich, RT;Reinhardt, HC;Quaas, A;Bogus, M;Courts, C;Nürnberg, P;Becker, K;Achter, V;Büttner, R;Wolf, J;Peifer, M;Thomas, RK; | Evolutionary trajectories of small cell lung cancer under therapy | Nature | 38480884 | onoraria for lectures and advisory boards for AbbVie, Amgen, AstraZeneca, Bayer, BMS, Boehringer Ingelheim, Illumina, Janssen, Lilly, Merck Serono, MSD, Novartis, Qiagen, Pfizer, Roche and Targos MP/ Discovery Life Sciences. R.B. serves as a member of the board of directors and is a shareholder of Gnothis, Inc. (SE). A.Q. is consultant to Astellas, BMS, MSD, AstraZeneca, Servier and Oncowissen App | Full Paper |
2024 | Bai, D;Zhang, X;Xiang, H;Guo, Z;Zhu, C;Yi, C; | Simultaneous single-cell analysis of 5mC and 5hmC with SIMPLE-seq | Nature biotechnology | 38336903 | MEM/F12 with 20% FBS and 1,000 U ml−1 LIF. Human PBMCs were purchased form AllCells (PB003F-C). Cultured cells or PBMCs were harvested and washed with fresh no-serum media (DM | Full Paper |
2024 | Hamilton, JR;Chen, E;Perez, BS;Sandoval Espinoza, CR;Kang, MH;Trinidad, M;Ngo, W;Doudna, JA; | In vivo human T cell engineering with enveloped delivery vehicles | Nature biotechnology | 38212493 | Cryopreserved PBMCs (AllCells) were thawed in X-VIVO 15 (Lonza) with 50 µM 2-mercaptoethanol (Gibco), 5% fetal bovine serum (VWR) and 10 mM N-acetyl L-cysteine (Sigma-Aldrich). This | Full Paper |
2024 | Mühlenberg, T;Falkenhorst, J;Schulz, T;Fletcher, BS;Teuber, A;Krzeciesa, D;Klooster, I;Lundberg, M;Wilson, L;Lategahn, J;von Mehren, M;Grunewald, S;Tüns, AI;Wardelmann, E;Sicklick, JK;Brahmi, M;Serrano, C;Schildhaus, HU;Sievers, S;Treckmann, J;Heinrich, MC;Raut, CP;Ou, WB;Marino-Enriquez, A;George, S;Rauh, D;Fletcher, JA;Bauer, S; | KIT ATP-Binding Pocket/Activation Loop Mutations in GI Stromal Tumor: Emerging Mechanisms of Kinase Inhibitor Escape | Journal of clinical oncology : official journal of the American Society of Clinical Oncology | 38408285 | Department of Medical Oncology and Sarcoma Center, West German Cancer Center, University Duisburg-Essen, Medical School, Essen, Germany.||DKTK partner site Essen, German Cancer Consortium (DKTK), Heidelberg, Germany.++Department of Medical Oncology and Sarcoma Center, West German Cancer Center, University Duisburg-Essen, Medical School, Essen, Germany.||DKTK partner site Essen, German Cancer Consortium (DKTK), Heidelberg, Germany.++Department of Chemistry and Chemical Biology, TU Dortmund University, Dortmund, Germany.||Drug Discovery Hub Dortmund (DDHD) am Zentrum für Integrierte Wirkstoffforschung (ZIW), Dortmund, Germany.++Department of Medical Oncology and Sarcoma Center, West German Cancer Center, University Duisburg-Essen, Medical School, Essen, Germany.||DKTK partner site Essen, German Cancer Consortium (DKTK), Heidelberg, Germany.++Department of Chemistry and Chemical Biology, TU Dortmund University, Dortmund, Germany.||Drug Discovery Hub Dortmund (DDHD) am Zentrum für Integrierte Wirkstoffforschung (ZIW), Dortmund, Germany.++Department of Medical Oncology and Sarcoma Center, West German Cancer Center, University Duisburg-Essen, Medical School, Essen, Germany.||DKTK partner site Essen, German Cancer Consortium (DKTK), Heidelberg, Germany.++Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.++Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.++Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.++Department of Chemistry and Chemical Biology, TU Dortmund University, Dortmund, Germany.||Drug Discovery Hub Dortmund (DDHD) am Zentrum für Integrierte Wirkstoffforschung (ZIW), Dortmund, Germany.++Department of Hematology and Oncology, Fox Chase Cancer Center, Temple Health System, University, Philadelphia, PA.++Department of Medical Oncology and Sarcoma Center, West German Cancer Center, University Duisburg-Essen, Medical School, Essen, Germany.||DKTK partner site Essen, German Cancer Consortium (DKTK), Heidelberg, Germany.++Laboratory of Molecular Oncology, Department of Medical Oncology, West German Cancer Center, University Hospital Essen, Essen, Germany.++Gerhard Domagk Institute of Pathology, University Hospital Münster, Münster, Germany.++Department of Surgery, Division of Surgical Oncology, University of California San Diego, San Diego, CA.||Department of Pharmacology, Moores Cancer Center, University of California San Diego, San Diego, CA.++Centre Leon Berard, Medical Oncology, Lyon, France.++Sarcoma Translational Research Laboratory, Vall d'Hebron Institute of Oncology (VHIO), Barcelona, Spain.||Medical Oncology Department, Vall d'Hebron University Hospital, Barcelona, Spain.++University Hospital Essen, Institute of Pathology, Essen, Germany.||Current affiliation: Discovery Life Sciences Biomarker Services & Institute of Pathology Nodhessen, Kassel, Germany.++Compound Management and Screening Center, Max Planck Institute of Molecular Physiology, Dortmund, Germany.++University of Duisburg-Essen, Medical School, Department of Visceral and Transplantation Surgery, Essen, Germany.++Portland VA Health Care System and OHSU Knight Cancer Institute, Portland, OR.++Department of Surgery, Brigham and Women's Hospital, Boston, MA.||Center for Sarcoma and Bone Oncology, Dana-Farber Cancer Institute, Boston, MA.||Harvard Medical School, Boston, MA.++Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.||Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, China.++Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.++Dana-Farber Cancer Institute, Medical Oncology, Boston, MA.++Department of Chemistry and Chemical Biology, TU Dortmund University, Dortmund, Germany.||Drug Discovery Hub Dortmund (DDHD) am Zentrum für Integrierte Wirkstoffforschung (ZIW), Dortmund, Germany.++Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA.++Department of Medical Oncology and Sarcoma Center, West German Cancer Center, University Duisburg-Essen, Medical School, Essen, Germany.||DKTK partner site Essen, German Cancer Consortium (DKTK), Heidelberg, Germany | Full Paper |
2024 | Sam, J;Hofer, T;Kuettel, C;Claus, C;Thom, J;Herter, S;Georges, G;Korfi, K;Lechmann, M;Eigenmann, MJ;Marbach, D;Jamois, C;Lechner, K;Krishnan, SM;Gaillard, BC;Marinho, J;Kronenberg, S;Kunz, L;Wilson, S;Briner, S;Gebhardt, S;Varol, A;Appelt, B;Nicolini, VG;Speziale, D;Bez, M;Bommer, E;Eckmann, J;Hage, C;Limani, F;Jenni, S;Schoenle, A;Le Clech, M;Vallier, JB;Colombetti, S;Bacac, M;Gasser, S;Klein, C;Umaña, P; | CD19-CD28: An affinity-optimized CD28 agonist for combination with glofitamab (CD20-TCB) as off-the-shelf immunotherapy | Blood | 38437725 | DLBCL PBMC samples were purchased from Discovery Life Sciences. A spleen sample from a B cell lymphoma patient was purchased from iSpecimen. PBMCs were | Full Paper |
2024 | Castiello, MC;Brandas, C;Ferrari, S;Porcellini, S;Sacchetti, N;Canarutto, D;Draghici, E;Merelli, I;Barcella, M;Pelosi, G;Vavassori, V;Varesi, A;Jacob, A;Scala, S;Basso Ricci, L;Paulis, M;Strina, D;Di Verniere, M;Sergi Sergi, L;Serafini, M;Holland, SM;Bergerson, JRE;De Ravin, SS;Malech, HL;Pala, F;Bosticardo, M;Brombin, C;Cugnata, F;Calzoni, E;Crooks, GM;Notarangelo, LD;Genovese, P;Naldini, L;Villa, A; | Exonic knockout and knockin gene editing in hematopoietic stem and progenitor cells rescues RAG1 immunodeficiency | Science translational medicine | 38324638 | ocyte colony-stimulating factor (G-CSF) or G-CSF+Plerixafor mPB CD34+ cells were purchased frozen from Lonza or purified in house with the CliniMACS CD34 Reagent System (Miltenyi Biotec) from Mobilized Leukopak (AllCells) according to the TIGET-HPCT protocol approved by OSR Ethical Committee and following the manufacturer’s instructions. mPB CD34+ cells were cultured for three days prior to editing procedure at the co | Full Paper |
2024 | Keller, MD;Schattgen, SA;Chandrakasan, S;Allen, EK;Jensen-Wachspress, MA;Lazarski, CA;Qayed, M;Lang, H;Hanley, PJ;Tanna, J;Pai, SY;Parikh, S;Berger, SI;Gottschalk, S;Pulsipher, MA;Thomas, PG;Bollard, CM; | Secondary bone marrow graft loss after third-party virus-specific T cell infusion: Case report of a rare complication | Nature communications | 38553461 | m; and Study Support—Adaptive, Miltenyi. PJH is a Co-founder and Board of Directors: Mana Therapeutics; and Scientific Advisory Board: Cellevolve, Cellenkos, Capsida, MicrofluidX, Discovery Life Sciences. MDK is an author for Elsevier (Uptodate), and has received research funding from Chiesi Pharmaceuticals | Full Paper |
2024 | Huang, L;Pang, D;Yang, H;Li, W;Wang, S;Cui, S;Liao, N;Wang, Y;Wang, C;Chang, YC;Wang, HC;Kang, SY;Seo, JH;Shen, K;Laohawiriyakamol, S;Jiang, Z;Wang, H;Lamour, F;Song, G;Curran, M;Duan, C;Lysbet de Haas, S;Restuccia, E;Shao, Z; | Neoadjuvant-adjuvant pertuzumab in HER2-positive early breast cancer: final analysis of the randomized phase III PEONY trial | Nature communications | 38461323 | in mainland China were analyzed at the Roche Oncology Biomarker Development laboratory (Shanghai, China). The patient samples from the rest of the world (Korea, Taiwan, and Thailand) were examined at Targos Molecular Pathology GmbH (Kassel, Germany). Study endpoints The primary efficacy outcome measure of PEONY was independent review committee-assessed tpCR (i.e. ypT0/is, ypN0 according to the cu | Full Paper |
2024 | Mahmoud, M;Huang, Y;Garimella, K;Audano, PA;Wan, W;Prasad, N;Handsaker, RE;Hall, S;Pionzio, A;Schatz, MC;Talkowski, ME;Eichler, EE;Levy, SE;Sedlazeck, FJ; | Utility of long-read sequencing for All of Us | Nature communications | 38281971 | Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA.||Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.++Data Sciences Platform, Broad Institute of MIT and Harvard, Cambridge, MA, 02141, USA.++Data Sciences Platform, Broad Institute of MIT and Harvard, Cambridge, MA, 02141, USA.++The Jackson Laboratory for Genomic Medicine, Farmington, CT, 06032, USA.++Data Sciences Platform, Broad Institute of MIT and Harvard, Cambridge, MA, 02141, USA.++Discovery Life Sciences, Huntsville, AL, 35806, USA.++Department of Genetics, Harvard Medical School, Boston, MA, USA.||Program in Medical and Population Genetics, Broad Institute of MIT and Harvard, Cambridge, MA, 02141, USA.++Discovery Life Sciences, Huntsville, AL, 35806, USA.++Discovery Life Sciences, Huntsville, AL, 35806, USA.++Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA.++Program in Medical and Population Genetics, Broad Institute of MIT and Harvard, Cambridge, MA, 02141, USA.||Center for Genomic Medicine, Massachusetts General Hospital, Boston, MA, USA.++Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.||Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL, 35806, USA.++Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA. fritz.sedlazeck@bcm.edu.||Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA. fritz.sedlazeck@bcm.edu.||Department of Computer Science, Rice University, Houston, TX, USA. fritz.sedlazeck@bcm.edu | Full Paper |
2024 | Bahal, S;Zinicola, M;Moula, SE;Whittaker, TE;Schejtman, A;Naseem, A;Blanco, E;Vetharoy, W;Hu, YT;Rai, R;Gomez-Castaneda, E;Cunha Santos, C;Burns, SO;Morris, EC;Booth, C;Turchiano, G;Cavazza, A;Thrasher, AJ;Santilli, G; | Hematopoietic stem cell gene editing rescues B cell development in X-linked agammaglobulinemia | The Journal of allergy and clinical immunology | 38479630 | CD34+ Hematopoietic Stem Cells (HSPCs) from healthy donors (AllCells) 154 and XLA patients were isolated as described in supplementary methods and cultured 155 in | Full Paper |
2024 | Prajapati, K;Yan, C;Yang, Q;Arbitman, S;Fitzgerald, DP;Sharee, S;Shaik, J;Bosiacki, J;Myers, K;Paucarmayta, A;Johnson, DM;O'Neill, T;Kundu, S;Cusumano, Z;Langermann, S;Langenau, DM;Patel, S;Flies, DB; | The FLRT3-UNC5B checkpoint pathway inhibits T cell-based cancer immunotherapies | Science advances | 38427724 | sc-514482) was used in the IHC procedure developed by Discovery Life Sciences (DLS; Newtown, PA), and outlined in Table 2 to stain FFPE human tissues from DLS | Full Paper |
2024 | Chan, AKN;Han, L;Delaney, CD;Wang, X;Mukhaleva, E;Li, M;Yang, L;Pokharel, SP;Mattson, N;Garcia, M;Wang, B;Xu, X;Zhang, L;Singh, P;Elsayed, Z;Chen, R;Kuang, B;Wang, J;Yuan, YC;Chen, B;Chan, LN;Rosen, ST;Horne, D;Müschen, M;Chen, J;Vaidehi, N;Armstrong, SA;Su, R;Chen, CW; | Therapeutic targeting Tudor domains in leukemia via CRISPR-Scan Assisted Drug Discovery | Science advances | 38394203 | at 37°C. For the colony-forming assay, the transduced cells were seed at a density of 1 × 104 per 35-mm culture dishes (27150, STEMCELL Technologies) in methylcellulose-based media (ColonyGEL 1201, ReachBio Research Labs) supplemented with murine recombinant IL-3 (10 ng/ml), IL-6, granulocyte-macrophage colony-stimulating factor, and murine recombinant SCF (50 ng/ml), along with G418 (1.0 mg/ml | Full Paper |
2024 | Li, Y;Xu, J;Chen, C;Lu, Z;Wan, D;Li, D;Li, JS;Sorg, AJ;Roberts, CC;Mahajan, S;Gallant, MA;Pinkoviezky, I;Cui, Y;Taggart, DJ;Li, W; | Multimodal epigenetic sequencing analysis (MESA) of cell-free DNA for non-invasive colorectal cancer detection | Genome medicine | 38225592 | at clinical sites within the USA through the ELITE Study (NCT05181826) or were obtained through the following contract research organizations: BioIVT (Westbury, NY, USA), BioOptions (Brea, CA, USA), Discovery Life Sciences (Boston, MA, USA), and DX Biosamples (San Diego, CA, USA). Cohort 2 comprised 129 patients diagnosed with colorectal cancer and 203 control individuals without colorectal cance | Full Paper |
2024 | Xiang, H;Pan, Y;Sze, MA;Wlodarska, M;Li, L;Van De Mark, KA;Qamar, H;Moure, CJ;Linn, DE;Hai, J;Huo, Y;Clarke, J;Tan, TG;Ho, S;Teng, KW;Ramli, MN;Nebozhyn, M;Zhang, C;Barlow, J;Gustafson, CE;Gornisiewicz, S;Albertson, TP;Korle, SL;Bueno, R;Moy, LY;Vollmann, EH;Chiang, DY;Brandish, PE;Loboda, A; | Single-cell analysis identifies NOTCH3-mediated interactions between stromal cells that promote microenvironment remodeling and invasion in lung adenocarcinoma | Cancer research | 38335304 | Cancer immunotherapy has revolutionized the treatment of lung adenocarcinoma (LUAD); however, a significant proportion of patients do not respond. Recent | Full Paper |
2024 | Sasine, JP;Kozlova, NY;Valicente, L;Dukov, J;Tran, DH;Himburg, HA;Kumar, S;Khorsandi, S;Chan, A;Grohe, S;Li, M;Kan, J;Sehl, ME;Schiller, GJ;Reinhardt, B;Singh, BK;Ho, R;Yue, P;Pasquale, EB;Chute, JP; | Inhibition of Ephrin B2 Reverse Signaling Abolishes Multiple Myeloma Pathogenesis | Cancer research | 38231476 | Deidentified, healthy control BM samples were obtained from AllCells. BM microenvironment cells and CD269+ (TNFRSF17/BCMA) MM cells were isolated from primary | Full Paper |
2024 | Huang, J;Gu, H;Orton, J;Sedova, M;Marcovitz, A;Burke, J;Brozio, S;Williams, P;Myrand, S;Olowo, N;Broomer, A;Deal, B;Seeger, C;Sadis, S;Rozenzhak, S;Hyland, F;Liu, G; | Abstract 7657: Detection of KMT2A-PTDs and KMT2A fusions using next generation sequencing | Cancer Research | We acquired 20 healthy donor whole blood samples (total 127 replicates) from Stanford TM Blood Center and Discovery Life Sciences TM and sequenced them at 3 | Full Paper | |
2024 | Mukker, J;Kulkarni, R;Huang, Y;Clarke, E;Gounaris, I;Zutshi, A;Venkatakrishnan, K;Tafuri, A; | Abstract 7186: In vitro evaluation of myelosuppressive effects of ATRi tuvusertib and ATMi lartesertib (M4076), alone and in combination | Cancer Research | 1EMD Serono, Billerica, MA;++1EMD Serono, Billerica, MA;++2Discovery Life Sciences, Seattle, WA;++2Discovery Life Sciences, Seattle, WA;++3Merck Serono Ltd. (an affiliate of Merck KGaA, Darmstadt, Germany), Feltham, United Kingdom;++1EMD Serono, Billerica, MA;++1EMD Serono, Billerica, MA;++4the healthcare business of Merck KGaA, Darmstadt, Germany | Full Paper | |
2024 | Gutierrez, D;Moore, J;Arivalagan, J;Louie, T;Wilkinson, L;Clemens, R; | Abstract 7092: Cancer biomarker discovery with scalable biofluid proteomics | Cancer Research | Aliquots of human plasma from 78 samples representing a range of cancer indications were obtained through the Discovery Life Sciences biobank and used for analysis | Full Paper | |
2024 | Karger, A;Patel, A;Tseng, K;Ornelas, C;Napier, M;Beck, R;Collwell, K;Fahl, S;Bornarth, C; | Abstract 6423: KRAS mutation detection by Sanger sequencing using the SeqStudio Flex capillary electrophoresis instrument | Cancer Research | Discovery Life Sciences has generated a large-scale biorepository of human biospecimen, including FFPE, plasma/sera, buffy coat, and PBMCs. Archival FFPE samples | Full Paper | |
2024 | Nguyen, P;Zhang, G;Kakrecha, B;Marable, R;Kirov, S;Bowden, M;Gjini, E; | Abstract 5627: PPARG-high circulating monocytes exhibit an immunosuppressive phenotype in urothelial cancer patients treated with anti-PD1 | Cancer Research | Real-world samples (Discovery Life Sciences) were collected from patients who received prior Pembrolizumab. Time post-treatment to collection ranged from 46 to 307 | Full Paper | |
2024 | Rock, R;Prasad, N;Robinson, M;Wildman, D;Montoya, O;Sykes, M;Umylyn, B;Halsey, T; | Abstract 324: Comparison of xGen Exome Hyb Panel v2 (IDT) kit and Twist Exome 2.0 (TWIST) kit for whole exome sequencing optimization from low input and degraded FFPE samples | Cancer Research | 1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL | Full Paper | |
2024 | Egidio, C;Bhosale, D;Shen, L;Jett, S;Malek, A;de Groot, T;Weaver, S;Sun, G;Travers, K;Facer, G; | Abstract 2930: Recovery of limited viable cells from low-quality precious samples for scRNA-seq | Cancer Research | of Levitation Technology using the LeviCell 1.0 system, 105 cryopreserved samples derived from diseased human tissues were processed by Discovery Life Sciences | Full Paper | |
2024 | Prasad, N;Rock, R;Beatty, R;Robinson, M;Wildman, D;Sykes, M;Umylny, B;Halsey, T; | Abstract 2298: Optimization of high-volume cell free DNA extraction and end-to-end automation for TSO 500 ctDNA library prep | Cancer Research | 1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL | Full Paper | |
2024 | Schildhaus, H;Badve, S;D’Arrigo, C;Farshid, G;Lebeau, A;Peg, V;Penault-Llorca, F;Rueschoff, J;Yang, W;Atkey, N;Baumann, J;Beyerlein, E;Newell, A;Penner, A;Moh, A;Viale, G; | Abstract 1030: Concordance between the DESTINY-Breast04 clinical trial assay (4B5[CDx]) and other HER2 IHC assays for HER2-low breast cancer in real-world practice: First phase of a large-scale, multicenter global ring study | Cancer Research | 1Discovery Life Sciences Tissue Biomarker Services GmbH, Kassel, Germany;++2Emory University School of Medicine, Atlanta, GA;++3Poundbury Cancer Institute, Dorchester, Dorset, United Kingdom;++4Royal Adelaide Hospital, Adelaide, Australia;++5University Medical Centre Hamburg-Eppendorf, Hamburg-Eppendorf, Germany;++6University Hospital Vall d’Hebron, Barcelona, Spain;++7Centre Jean Perrin, Université Clermont Auvergne, INSERM, Clermont-Ferrand, France;++1Discovery Life Sciences Tissue Biomarker Services GmbH, Kassel, Germany;++8Fudan University, Shanghai, China;++9Diaceutics PLC, Belfast, United Kingdom;++10AstraZeneca, Gaithersburg, MD;++11Daiichi Sankyo Europe GmbH, Munich, Germany;++12Daiichi Sankyo Inc., Basking Ridge, NJ;++1Discovery Life Sciences Tissue Biomarker Services GmbH, Kassel, Germany;++12Daiichi Sankyo Inc., Basking Ridge, NJ;++13IEO European Institute of Oncology IRCCS, Milan, Italy | Full Paper | |
2024 | Koo, D;Mao, Z;Dimatteo, R;Noguchi, M;Tsubamoto, N;McLaughlin, J;Tran, W;Lee, S;Cheng, D;de Rutte, J;Burton Sojo, G;Witte, ON;Di Carlo, D; | Defining T cell receptor repertoires using nanovial-based binding and functional screening | Proceedings of the National Academy of Sciences of the United States of America | 38536748 | To prime naive T cells with peptides of interest, PBMCs from commercial vendors (AllCells) were cultured and processed as previously described with chemically synthesized peptides (80% purity, Elim Biopharm) (19).K562 and Jurkat-NFAT- ZsGree | Full Paper |
2024 | Tian, J;Ashique, AM;Weeks, S;Lan, T;Yang, H;Chen, HH;Song, C;Koyano, K;Mondal, K;Tsai, D;Cheung, I;Moshrefi, M;Kekatpure, A;Fan, B;Li, B;Qurashi, S;Rocha, L;Aguayo, J;Rodgers, C;Meza, M;Heeke, D;Medfisch, SM;Chu, C;Starck, S;Basak, NP;Sankaran, S;Malhotra, M;Crawley, S;Tran, TT;Duey, DY;Ho, C;Mikaelian, I;Liu, W;Rivera, LB;Huang, J;Paavola, KJ;O'Hollaren, K;Blum, LK;Lin, VY;Chen, P;Iyer, A;He, S;Roda, JM;Wang, Y;Sissons, J;Kutach, AK;Kaplan, DD;Stone, GW; | ILT2 and ILT4 drive myeloid suppression via both overlapping and distinct mechanisms | Cancer immunology research | 38393969 | For LPS spheroid assays, PBMCs (Allcells Inc) were cultured on Matrigel matrix in media (RPMI with 10% FBS, L-glutamine, and pen/strep; all from Gibco) at 37 C for 2;- Solid tumors are dense three-dimensional (3D) multicellular structures that enable efficient receptor-ligand trans interactions via close cell-cell contact. Immunoglobulin | Full Paper |
2024 | Degagné, É;Donohoue, PD;Roy, S;Scherer, J;Fowler, TW;Davis, RT;Reyes, GA;Kwong, G;Stanaway, M;Larroca Vicena, V;Mutha, D;Guo, R;Edwards, L;Schilling, B;Shaw, M;Smith, SC;Kohrs, B;Kufeldt, HJ;Churchward, G;Ruan, F;Nyer, DB;McSweeney, K;Irby, MJ;Fuller, CK;Banh, L;Toh, MS;Thompson, M;Owen, ALG;An, Z;Gradia, S;Skoble, J;Bryan, M;Garner, E;Kanner, SB; | High-specificity CRISPR-mediated genome engineering in anti-BCMA allogeneic CAR T cells suppresses allograft rejection in preclinical models | Cancer immunology research | 38345397 | NK cells were isolated from a fresh leukopak (AllCells) using a human NK-cell isolation kit (Miltenyi Biotec) in conjunction with the QuadroMACS Separator (Miltenyi Biotec);- For multiple myeloma bone marrow mononuclear cell (BMNC) samples (Discovery Life Sciences), in addition to propidium iodide (PI) staining (BioLegend), samples were | Full Paper |
2024 | Rogers, BB;Anderson, AG;Lauzon, SN;Davis, MN;Hauser, RM;Roberts, SC;Rodriguez-Nunez, I;Trausch-Lowther, K;Barinaga, EA;Hall, PI;Knuesel, MT;Taylor, JW;Mackiewicz, M;Roberts, BS;Cooper, SJ;Rizzardi, LF;Myers, RM;Cochran, JN; | Neuronal MAPT expression is mediated by long-range interactions with cis-regulatory elements | American journal of human genetics | 38232730 | HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA; University of Alabama at Birmingham, Birmingham, AL 35294, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA. Electronic address: rmyers@hudsonalpha.org.++HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA. Electronic address: ncochran@hudsonalpha.org | Full Paper |
2024 | Delaney-Busch, N;Cowan, A;Haverty, C;Khodursky, A;Jain, M;Namsaraev, E;Abbas, I;Moe, A;Mekaru, K;Reddy, M;Rasmussen, M; | 293 Cell-free RNA detection of | American Journal of Obstetrics and Gynecology | NP individuals (N=49) were 32 (22-40) years, BMI 32 (19-61) and were collected by Discovery Life Sciences. All samples were collected and processed using the same | Full Paper | |
2024 | Qi, T;Cohen, A;Miguel, C;Peskin-Stolze, M;Dar, P;Doulaveris, G; | 291 Association of resolving placenta previa and other early sonographic markers with postpartum hemorrhage | American Journal of Obstetrics and Gynecology | NP individuals (N¼49) were 32 (22-40) years, BMI 32 (19-61) and were collected by Discovery Life Sciences. All samples were collected and processed using the same | Full Paper | |
2023 | Jiang, W;Gu, G;Zhang, Y;Song, Y;Shi, M;Wang, G;Li, H;Tao, T;Qin, J;Li, X;Jia, H;Jiao, F;Xu, W;Huang, X; | Novel mesothelin-targeted chimeric antigen receptor-modified UNKT cells are highly effective in inhibiting tumor progression | Pharmacological research | 37775021 | purchased from Allcells according to the method described by Garcia et al [288] . Collection protocols and donor informed-consent were approved by an IRB at Allcells and | Full Paper |
2024 | Rivera, M;Zhang, H;Pham, J;Isquith, J;Zhou, QJ;Balaian, L;Sasik, R;Enlund, S;Mark, A;Ma, W;Holm, F;Fisch, KM;Kuo, DJ;Jamieson, C;Jiang, Q; | Malignant A-to-I RNA editing by ADAR1 drives T cell acute lymphoblastic leukemia relapse via attenuating dsRNA sensing | Cell reports | 38265938 | rd approved protocol (#130794) that meets the requirements as stated in 45 CFR 46.404 and 21 CFR 50.51. De-identified (IRB exempt) human cord blood samples were purchased as purified CD34+ cells from AllCells Inc or StemCell Techologies Inc. Detailed patient information can be found in Table S2. Animal models and experiments All mouse studies were conducted under protocols approved by the Institu | Full Paper |
2024 | Zaun, G;Borchert, S;Metzenmacher, M;Lueong, S;Wiesweg, M;Zaun, Y;Pogorzelski, M;Behrens, F;Schildhaus, HU;Virchow, I;Kasper, S;Schuler, M;Theurer, S;Liffers, S; | Comprehensive biomarker diagnostics of unfavorable cancer of unknown primary to identify patients eligible for precision medical therapies | European journal of cancer (Oxford, England : 1990) | 38316065 | West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany. Electronic address: gregor.zaun@uk-essen.de.++West German Cancer Center, Institute of Pathology Essen, University Hospital Essen, Germany.++West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany.++German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany; West German Cancer Center, Institute for Developmental Cancer Therapeutics, University Hospital Essen, Essen, Germany.++West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany; National Center for Tumor Diseases (NCT) West, Essen, Germany.++West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany.++West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany.++West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany.++West German Cancer Center, Institute of Pathology Essen, University Hospital Essen, Germany; Discovery Life Sciences, Kassel, Germany.++West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany; National Center for Tumor Diseases (NCT) West, Essen, Germany.++West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany; National Center for Tumor Diseases (NCT) West, Essen, Germany.++West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany; National Center for Tumor Diseases (NCT) West, Essen, Germany.++West German Cancer Center, Institute of Pathology Essen, University Hospital Essen, Germany.++German Cancer Consortium (DKTK), Partner site University Hospital Essen, Essen, Germany; West German Cancer Center, Institute for Developmental Cancer Therapeutics, University Hospital Essen, Essen, Germany | Full Paper |
2024 | Kink, JA;Bellio, MA;Forsberg, MH;Lobo, A;Thickens, AS;Lewis, BM;Ong, IM;Khan, A;Capitini, CM;Hematti, P; | Large-scale bioreactor production of extracellular vesicles from mesenchymal stromal cells for treatment of acute radiation syndrome | Stem cell research & therapy | 38475968 | iquoted, then stored at − 80 °C. Bioreactor cultivation of MSCs MSCs were derived from Good Manufacturing Practice (GMP)-compliant human BM aspirates from a healthy donor (B_1) purchased from AllCells, (Alameda, CA, USA) and grown in MSC culture media described above but was supplemented with a more GMP-compliant xenogen-free, 5% human platelet lysate (hPL) (Millcreek, Rochester MN, USA) i | Full Paper |
2024 | Inui, T;Uraya, Y;Yokota, J;Yamashita, T;Kawai, K;Okada, K;Ueyama-Toba, Y;Mizuguchi, H; | Functional intestinal monolayers from organoids derived from human iPS cells for drug discovery research | Stem cell research & therapy | 38424603 | Primary cryopreserved human small intestinal cells (lot; CHIM6005, ileum of male donors aged 20) were purchased from Discovery Life Sciences. Just after thawing, they | Full Paper |
2024 | Wistinghausen, B;Toner, K;Barkauskas, DA;Jerkins, LP;Kinoshita, H;Chansky, P;Pezzella, G;Saguilig, L;Hayashi, RJ;Abhyankar, H;Scull, B;Karri, V;Tanna, J;Hanley, P;Hermiston, ML;Allen, CE;Bollard, CM; | Durable immunity to EBV after rituximab and third-party LMP-specific T cells: a Children's Oncology Group study | Blood advances | 38163318 | 00004328Genentech/10.13039/100004337Roche who manufacture rituximab. P.H. is a cofounder and on the board of directors of Mana Therapeutics; and serves on the adviser boards of Cellevolve, Cellenkos, Discovery Life Sciences, Capsida, and MicrofluidX. The remaining authors declare no competing financial interests. References 1 WistinghausenBGrossTGBollardCPost-transplant lymphoproliferative diseas;- PH is a cofounder and on the board of directors of Mana Therapeutics; and serves on the adviser boards of Cellevolve, Cellenkos, Discovery Life Sciences, Capsida, and | Full Paper |
2024 | Yang, Y;Vedvyas, Y;Alcaina, Y;Son, JY;Min, IM;Jin, MM; | Low-dose targeted radionuclide therapy synergizes with CAR T cells and enhances tumor response | Frontiers in immunology | 38550578 | To manufacture CAR T cells, leukopaks were obtained either from healthy donors (AllCells) commercially or from advanced thyroid cancer (ATC) patients who provided | Full Paper |
2024 | Al-Toubah, T;Schell, MJ;Morse, B;Haider, M;Valone, T;Strosberg, J; | Phase II study of pembrolizumab and lenvatinib in advanced well-differentiated neuroendocrine tumors | ESMO open | 38507897 | atment for the first year, then every 12 weeks for the second year. RECIST version 1.1 was used for evaluation of primary endpoint of response rate. Optional archival tissue was collected and sent to QualTek for correlative analysis of PD-L1 tumor expression, TMB, and microsatellite instability status. Sample size calculation The primary endpoint of the trial was objective radiographic response r | Full Paper |
2024 | Tan, Y;Mosallanejad, K;Zhang, Q;O'Brien, S;Clements, M;Perper, S;Wilson, S;Chaulagain, S;Wang, J;Abdalla, M;Al-Saidi, H;Butt, D;Clabbers, A;Ofori, K;Dillon, B;Harvey, B;Memmott, J;Negron, C;Winarta, D;Tan, C;Biswas, A;Dong, F;Morales-Tirado, V;Lu, X;Singh, G;White, M;Ashley, S;Knight, H;Westmoreland, S;Phillips, L;Carr, T;Reinke-Breen, L;Singh, R;Xu, J;Wu, K;Rinaldi, L;Stoll, B;He, YD;Hazelwood, L;Karman, J;McCluskey, A;Stine, W;Correia, I;Gauld, S;Levesque, MC;Veldman, G;Hubeau, C;Radstake, T;Sadhukhan, R;Fiebiger, E; | IL11-mediated stromal cell activation may not be the master regulator of pro-fibrotic signaling downstream of TGFβ | Frontiers in immunology | 38455057 | biopsies of the unaffected adjacent tissue to the resection were used. Tissues were also acquired from commercial vendors (Folio, Discovery Life Sciences, and Celerity Biosciences) | Full Paper |
2024 | Kelbauskas, L;Legutki, JB;Woodbury, NW; | Highly heterogenous humoral immune response in Lyme disease patients revealed by broad machine learning-assisted antibody binding profiling with random peptide arrays | Frontiers in immunology | 38318184 | icroarrays. The patient samples for the diseases with similar etiology to LD as well as non-endemic healthy controls were obtained from several commercial sources (Boca Biolistics, Pompano Beach, FL, Discovery Life Sciences, Huntsville, AL, Creative Testing Solutions, Tempe, AZ, SeraCare, Milford, MA). Note that these samples were obtained from commercial biobanks with no data about their previous | Full Paper |
2023 | Iadonato, S;Ovechkina, Y;Lustig, K;Cross, J;Eyde, N;Frazier, E;Kabi, N;Katz, C;Lance, R;Peckham, D;Sridhar, S;Talbaux, C;Tihista, I;Xu, M;Guillaudeux, T; | A highly potent anti-VISTA antibody KVA12123 - a new immune checkpoint inhibitor and a promising therapy against poorly immunogenic tumors | Frontiers in immunology | 38152397 | CD14+ monocytes were incubated with autologous human PBMCs (AllCells) and 10 µg/mL KVA mAbs. After 24-hour incubation, cells were harvested and stained with the | Full Paper |
2023 | Fiumara, M;Ferrari, S;Omer-Javed, A;Beretta, S;Albano, L;Canarutto, D;Varesi, A;Gaddoni, C;Brombin, C;Cugnata, F;Zonari, E;Maria Naldini, M;Barcella, M;Gentner, B;Merelli, I;Naldini, L; | P1373: UNCOVERING UPSIDES AND PITFALLS OF BASE AND PRIME EDITING IN HEMATOPOIETIC STEM CELLS | HemaSphere | G-CSF or G-CSF + Plerixafor mPB CD34+ HSPCs were purified in house with the CliniMACS CD34 Reagent System (Miltenyi Biotec) from Mobilized Leukopak (AllCells) according to the TIGET-HPCT protocol approved by OSR Ethical Committee and following the manufactu | Full Paper | |
2024 | Gay, CL;Hanley, PJ;Falcinelli, SD;Kuruc, JD;Pedersen, SM;Kirchherr, J;Raines, SLM;Motta, CM;Lazarski, C;Chansky, P;Tanna, J;Shibli, A;Datar, A;McCann, CD;Sili, U;Ke, R;Eron, JJ;Archin, N;Goonetilleke, N;Bollard, CM;Margolis, DM; | The Effects of Human Immunodeficiency Virus Type 1 (HIV-1) Antigen-Expanded Specific T-Cell Therapy and Vorinostat on Persistent HIV-1 Infection in People With HIV on Antiretroviral Therapy | The Journal of infectious diseases | 38349333 | PJH is a co-founder and serves on the board of directors of Mana Therapeutics and is an advisor to Cellenkos, Cellevolve, Discovery Life Sciences, Capsida, and | Full Paper |
2024 | Rajasekaran, N;Wang, X;Ravindranathan, S;Chin, DJ;Tseng, SY;Klakamp, SL;Widmann, K;Kapoor, VN;Vexler, V;Keegan, P;Yao, S;LaVallee, T;Khare, SD; | Toripalimab, a therapeutic monoclonal anti-PD-1 antibody with high binding affinity to PD-1 and enhanced potency to activate human T cells | Cancer immunology, immunotherapy : CII | 38400933 | IAShredders, and RNA quality and quantity were assessed on an Agilent TapeStation 4150 using the High Sensitivity RNA ScreenTape (Cat No. 5067-5581). The cDNA libraries from the RNA were sequenced by Discovery Life Sciences (DLS), Goleta, California. The concentration of the extracted RNAs were quantified using the fluorometric Ribogreen assay (Invitrogen, Cat No. R11490). The integrity of the ext;- The cDNA libraries from the RNA were sequenced by Discovery Life Sciences (DLS), Goleta, California. The concentration of the extracted RNAs were quantified using the | Full Paper |
2024 | Blitzer, GC;Paz, C;Glassey, A;Ganz, OR;Giri, J;Pennati, A;Meyers, RO;Bates, AM;Nickel, KP;Weiss, M;Morris, ZS;Mattison, RJ;McDowell, KA;Croxford, E;Chappell, RJ;Glazer, TA;Rogus-Pulia, NM;Galipeau, J;Kimple, RJ; | Functionality of bone marrow mesenchymal stromal cells derived from head and neck cancer patients - A FDA-IND enabling study regarding MSC-based treatments for radiation-induced xerostomia | Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology | 38224919 | Bone marrow from healthy adult control volunteers was purchased from a commercial source (AllCells, Alameda, CA). We had originally planned to enroll 15 patients: 5 | Full Paper |
2024 | Huang, L;Wang, Z;Liao, C;Zhao, Z;Gao, H;Huang, R;Chen, J;Wu, F;Zeng, F;Zhang, Y;Jiang, T;Hu, H; | PVT1 promotes proliferation and macrophage immunosuppressive polarization through STAT1 and CX3CL1 regulation in glioblastoma multiforme | CNS neuroscience & therapeutics | 38287522 | 4 MATERIALS AND METHODS 4.1 Cell culture U87, U251, HS683, and THP1 cell lines were obtained from the National Biomedical Experimental Cell Resource Bank, while CD14+ monocytes were purchased from AllCells Inc. U118 and LN229 cell lines were previously maintained in our laboratory. U87, HS683, U251, U118, and LN229 cells were cultured in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gi | Full Paper |
2024 | Frey, G;Cugnetti, APG;Liu, H;Xing, C;Wheeler, C;Chang, HW;Boyle, WJ;Short, JM; | A novel conditional active biologic anti-EpCAM x anti-CD3 bispecific antibody with synergistic tumor selectivity for cancer immunotherapy | mAbs | 38445633 | 106 HCT116 cells subcutaneously in the right front flank. Two hours after tumor cells inoculation, mice were engrafted intraperitoneally with non-activated 5 × 106 huPBMCs from healthy donors (Allcells, Beijing, China). When tumor volume was approximately 130 mm3, mice were randomized in different study groups and treatment with EpCAM x CD3 bispecific antibodies was initiated. For the BT4 | Full Paper |
2024 | Fobare, S;Elgamal, OA;Wunderlich, M;Stahl, E;Mehmood, A;Furby, C;Lerma, JR;Sesterhenn, TM;Pan, J;Rai, J;Johnstone, ME;Abdul-Aziz, A;Johnson, ML;Rai, SN;Byrd, JC;Hertlein, E; | Inhibition of Enhancer of Zeste Homolog 2 Induces Blast Differentiation, Impairs Engraftment and Prolongs Survival in Murine Models of Acute Myeloid Leukemia | Cancers | 38339323 | on the Revolve Microscope (ECHO). Samples that produced fewer than 20 colonies counted in the DMSO condition were excluded. For the normal donor CD34+ CFU assays, normal bone marrow was obtained from AllCells. The Human CD34 MicroBead Kit (Miltenyi Biotec, Gaithersburg, MD, USA) was used to isolate CD34+ cells. A total of 1000 CD34+ cells/well were plated with DMSO or 1 µM EPZ in MethoCult H4035 | Full Paper |
2024 | Mulato, A;Lansdon, E;Aoyama, R;Voigt, J;Lee, M;Liclican, A;Lee, G;Singer, E;Stafford, B;Gong, R;Murray, B;Chan, J;Lee, J;Xu, Y;Ahmadyar, S;Gonzalez, A;Cho, A;Stepan, GJ;Schmitz, U;Schultz, B;Marchand, B;Brumshtein, B;Wang, R;Yu, H;Cihlar, T;Xu, L;Yant, SR; | Preclinical characterization of a non-peptidomimetic HIV protease inhibitor with improved metabolic stability | Antimicrobial agents and chemotherapy | 38380945 | healthy volunteers participating in an Institute Review Board (IRB) approved donor program, negative for HIV-1, hepatitis B, and hepatitis C viral infection (AllCells, Inc., | Full Paper |
2024 | Uyar, D;Michener, CM;Bishop, E;Hopp, E;Simpson, P;Zhang, L;Rader, JS;Rose, PG;Mahdi, HS;Debernardo, R;Christian, Q;Bradley, W; | Carboplatin, paclitaxel, and pembrolizumab followed by pembrolizumab maintenance for primary treatment of incompletely resected epithelial ovarian cancer | Frontiers in oncology | 38410102 | PD-L1 staining was centrally performed by Qualtek Laboratories using the Merck 22C3 antibody for PD-L1 and reported through a modified percent score (MPS) ranging | Full Paper |
2024 | Murakami, T;Minami, K;Harabayashi, T;Maruyama, S;Takada, N;Kashiwagi, A;Miyata, H;Sato, Y;Matsumoto, R;Kikuchi, H;Abe, T;Ito, YM;Murai, S;Shinohara, N;Harada, H;Osawa, T; | Cross-sectional and longitudinal analyses of urinary extracellular vesicle mRNA markers in urothelial bladder cancer patients | Scientific reports | 38514751 | tional CSV, an independent validation cohort of 30 UBC patients was recruited prospectively in Central Europe between June 2021 and November 2021 through a commercial biospecimen procurement service (Discovery Life Sciences, IRB approval no. DLS-BB044-V.1). Clinical data including pathology and urine cytology results and urine samples were obtained as described above. EV mRNA assay EV mRNA assay;- We thank for Discovery Life Sciences team to support this study by recruiting patients and organizing their clinical specimen and clinical data. We thank for Showa Denko | Full Paper |
2024 | Trivedi, A;Lin, M;Miyazawa, B;Nair, A;Vivona, L;Fang, X;Bieback, K;Schäfer, R;Spohn, G;McKenna, D;Zhuo, H;Matthay, M;Pati, S; | Inter- and Intra-donor Variability in Bone Marrow-Derived Mesenchymal Stromal Cells: Implications for Clinical Applications | Cytotherapy | Allogeneic MSCs derived from human BM were prepared from three donors (aged 18-45 years, one woman and two men) after informed consent (AllCells, Alameda, CA) with support from the National Heart, Lung, and Blood Institute Production Assistance for Cellular Therapies Program at the University of | Full Paper | |
2024 | Melocchi, A;Schmittlein, B;Jones, A;Ainane, Y;Rizvi, A;Chan, D;Dickey, E;Pool, K;Harsono, K;Szymkiewicz, D;Scarfogliero, U;Bhatia, V;Sivanantham, A;Kreciglowa, N;Hunter, A;Gomez, M;Tanner, A;Uboldi, M;Batish, A;Balcerek, J;Kutova-Stoilova, M;Paruthiyil, S;Acevedo, L;Stadnitskiy, R;Carmichael, S;Aulbach, H;Hewitt, M;Jeu, X;Robilant, B;Parietti, F;Esensten, J; | Development of a robotic cluster for automated and scalable cell therapy manufacturing | Cytotherapy | Human CD8+ T cells isolated from commercially available human leukapheresis collections (StemExpress, US-CA or AllCells, US-CA) using Miltenyi CD8+ microbeads and a Miltenyi Prodigy instrument were used for experiment 1. Purified human CD8+ T cells were pu | Full Paper | |
2024 | Stewart, M;Kalos, M;Coutinho, V;Better, M;Jazayeri, J;Yohrling, J;Jadlowsky, J;Fuchs, M;Gidwani, S;Goessl, C;Hanley, P;Healy, J;Liu, W;McKelvey, B;Pearce, L;Pilon-Thomas, S;Andrews, H;Veldman, M;Vong, J;Weinbach, S;Allen, J; | Accelerating The Development of Genetically Engineered Cellular Therapies: A Framework for Extrapolating Data Across Related Products | Cytotherapy | f Janssen R&D, LLC. M.F is an employee of Novartis. S.G. is an employee of Allogene Therapeutics. C.G. is an employee of GSK. P.J.H. is a Scientific Advisor or Advisory Board for Cellenkos, Cellevolve, Discovery Life Sciences, Microfluidx, Autolomous, Capsida and is co-founder, board of directors of Mana Therapeutics. J.H. is an employee of Merck & Co. Inc. W. L. is a | Full Paper | |
2024 | Krivoshik, SR;Dzielak, L;Masters, AR;Hall, J;Johnson, AJ; | Development of an Enzyme-Linked Immunosorbent Spot Assay for the Assessment of Adeno-Associated Virus Peptides to Examine Immune Safety | Human gene therapy | 38264994 | PBMCs were isolated from whole blood donors (Sanguine, Woburn, MA; AllCells Leukopaks, Alameda, CA) using Ficoll-Paque (Cytiva, Marlborough, MA) and frozen in CryStor CS10 Commercial Freezing Media (BioLife Solutions, Bothell, WA) at −140°C. All donors were adults and include both men and women | Full Paper |
2024 | Wylezinski, L;Sesler, C;Shaginurova, G;Grigorenko, E;Wohlgemuth, J;Cockerill, F;Racke, M;Spurlock, C; | Machine Learning Analysis Using RNA Sequencing to Distinguish Neuromyelitis Optica from Multiple Sclerosis and Identify Therapeutic Candidates | The Journal of molecular diagnostics : JMD | Healthy control individuals with no chronic or acute illness and no family history of autoimmune disease were recruited from Discovery Life Sciences (Huntsville, AL) | Full Paper | |
2024 | Aluri, KC;Slavsky, M;Tan, Y;Whitcher-Johnstone, A;Zhang, Z;Hariparsad, N;Ramsden, D; | Aminobenzotriazole inhibits and induces several key drug metabolizing enzymes complicating its utility as a pan CYP inhibitor for reaction phenotyping | Clinical and translational science | 38501263 | For alternative potential inhibitors, the induction assessment was conducted using a single female donor HH1103 (In Vitro ADMET Laboratories, LLC), which has been | Full Paper |
2024 | Henrich, LM;Greimelmaier, K;Wessolly, M;Klopp, NA;Mairinger, E;Krause, Y;Berger, S;Wohlschlaeger, J;Schildhaus, HU;Baba, HA;Mairinger, FD;Borchert, S; | The Impact of Cancer-Associated Fibroblasts on the Biology and Progression of Colorectal Carcinomas | Genes | 38397199 | Institute of Pathology, University Hospital Essen, University of Duisburg Essen, 45147 Essen, Germany.++Department of Pathology, Diakonissenkrankenhaus Flensburg, 24939 Flensburg, Germany.++Institute of Pathology, University Hospital Essen, University of Duisburg Essen, 45147 Essen, Germany.++Institute of Pathology, University Hospital Essen, University of Duisburg Essen, 45147 Essen, Germany.++Institute of Pathology, University Hospital Essen, University of Duisburg Essen, 45147 Essen, Germany.++Institute of Pathology, University Hospital Essen, University of Duisburg Essen, 45147 Essen, Germany.++Institute of Pathology, University Hospital Essen, University of Duisburg Essen, 45147 Essen, Germany.++Department of Pathology, Diakonissenkrankenhaus Flensburg, 24939 Flensburg, Germany.++Targos-A Discovery Life Sciences Company, Germaniastraße 7, 34119 Kassel, Germany.||Institute of Pathology Nordhessen, Germaniastraße 7, 34119 Kassel, Germany.++Institute of Pathology, University Hospital Essen, University of Duisburg Essen, 45147 Essen, Germany.++Institute of Pathology, University Hospital Essen, University of Duisburg Essen, 45147 Essen, Germany.++Institute of Pathology, University Hospital Essen, University of Duisburg Essen, 45147 Essen, Germany | Full Paper |
2024 | Shimatani, K;Sato, H;Mizukami, K;Saito, A;Sasai, M;Enmi, JI;Watanabe, K;Kamohara, M;Yoshioka, Y;Miyagawa, S;Sawa, Y; | Transplantation of Human Embryonic Stem Cell-Derived Pericyte-Like Cells Transduced with Basic Fibroblast Growth Factor Promotes Angiogenic Recovery in Mice with Severe Chronic Hindlimb Ischemia | Journal of cardiovascular translational research | 38376701 | The purification method has been described previously [12]. BMMSCs and HUVECs were purchased from PromoCell Gmbh. Human PBMCs were purchased from AllCells LLC | Full Paper |
2024 | Wei, J;Chaney, K;Shim, WJ;Chen, H;Leonard, G;O'Brien, S;Liu, Z;Jiang, J;Ulrey, R; | Cryopreserved leukapheresis material can be transferred from controlled rate freezers to ultracold storage at warmer temperatures without affecting downstream CAR-T cell culture performance and in-vitro functionality | Cryobiology | 38513998 | Leukapheresis Reformulation: Fresh leukapheresis was acquired from healthy donors through multiple vendors (Discovery Life Sciences, StemExpress, and StemCell | Full Paper |
2024 | Tatsumi, Y;Kawaki, H;Shintani, K;Ueno, K;Hotta, M;Kondoh, N;Burrow, MF;Nikaido, T; | Bioactivity of human dental pulp-derived stem cells with boron-controlled S-PRG filler eluate by anion exchange | Dental materials journal | 38432951 | In this study, hDPSCs (Allcells) were cultured using MEM containing 10% FBS, 1 U/mL penicillin, and 100 μg/mL streptomycin at 37C and 5% CO2. The cells were seeded | Full Paper |
2024 | Condic, M;Rohr, A;Riemann, S;Staerk, C;Ayub, TH;Doeser, A;Zillinger, T;Merkelbach-Bruse, S;Buettner, R;Barchet, W;Rudlowski, C;Mustea, A;Kübler, K; | Immune Profiling of Vulvar Squamous Cell Cancer Discovers a Macrophage-rich Subtype Associated with Poor Prognosis | Cancer research communications | 38407373 | hringer-Ingelheim, Daiichi, AstraZeneca, Lilly, Novartis, Illumina, BMS, and Merck during the conduct of the study; and R. Buettner is a co-founder and co-owner of Targos Inc (Kassel/GE): now sold to Discovery Life Sciences, Gnothis Inc (Stockholm/SE): Developing Sequencing technologies, Timer Therapeutics (Fulda & Freiburg/GE): Developing drugs for cancer treatment. No disclosures were reported b | Full Paper |
2024 | Bitting, RL;Tooze, JA;Goodman, M;Vile, DC;Brown, JM;Thomas, CY;Neve, M;Kooshki, M;Addo, S;Triozzi, PL;Dubey, P; | Low-dose Paclitaxel with Pembrolizumab Enhances Clinical and Immunologic Responses in Platinum-refractory Urothelial Carcinoma | Cancer research communications | 38345536 | tained within 6 months of treatment on study. Five unstained ProbeOn Plus slides per patient, cut from a formalin-fixed, paraffin-embedded tissue block, were sent to Discovery Life Sciences (formerly QualTek Molecular Labs) for PD-L1 staining. As per the Merck Investigator Study Program, PD-L1 membrane expression was measured on tumor cells and mononuclear inflammatory cells (MIC) using the anti-P;- Five unstained ProbeOn Plus slides per patient, cut from a formalin-fixed, paraffin-embedded tissue block, were sent to Discovery Life Sciences (formerly QualTek | Full Paper |
2024 | Ligero, M;Serna, G;El Nahhas, OSM;Sansano, I;Mauchanski, S;Viaplana, C;Calderaro, J;Toledo, RA;Dienstmann, R;Vanguri, RS;Sauter, JL;Sanchez-Vega, F;Shah, SP;Ramón Y Cajal, S;Garralda, E;Nuciforo, P;Perez-Lopez, R;Kather, JN; | Weakly Supervised Deep Learning Predicts Immunotherapy Response in Solid Tumors Based on PD-L1 Expression | Cancer research communications | 38126740 | ovartis, Roche, Sanofi, SeaGen, Seattle Genetics, Thermo Fisher Scientific outside the submitted work; and stock from TRIALSP and employment from NEXT Oncology. P. Nuciforo reports personal fees from Discovery Life Sciences outside the submitted work. R. Perez-Lopez reports grants from AstraZeneca and Roche outside the submitted work; and R. Perez-Lopez is supported by LaCaixa Foundation, a CRIS F;- Nuciforo reports personal fees from Discovery Life Sciences outside the submitted work. R. Perez-Lopez reports grants from AstraZeneca and Roche outside the submitted | Full Paper |
2023 | Zhu, Y;Tahara, SM;Tsukamoto, H;Machida, K; | Protocol for generation of humanized HCC mouse model and cancer-driver mutations using CRISPR-Cas9 | STAR protocols | 38103196 | Human frozen Bone marrow CD34+ cells: (2.5 million cells): one vial of 2.5 million BM-CD34+ AllCells cat. no. ABM017F | Full Paper |
2024 | Knisely, A;Hinchcliff, E;Gardiner, E;Rangwala, R;Lito, K;Fellman, B;Yuan, Y;Sood, A;Westin, S;Lu, K;Jazaeri, A; | Phase 1b Study of Batiraxcept (AVB-S6-500) in Combination with Durvalumab (MEDI4736) in Patients with Platinum-Resistant Ovarian Cancer | Available at SSRN | Background: Combining an immune checkpoint inhibitor with Batiraxcept (AVB-S6-500), an AXL inhibitor that acts via selective binding to growth arrest-specific protein 6 ( | Full Paper | |
2024 | Melero, I;Luken, M;de Velasco, G;Garralda, E;Liberal, J;Joerger, M;Alonso, G;Goebeler, M;Schuler, M;König, D;Dummer, R;Reig, M;Rodriguez-Ruiz, M;Calvo, E;Esteban-Villarrubia, J;Oberoi, A;Sàbat, P;Soto-Castillo, J;Koster, K;Saavedra, O;Sayehli, C;Gromke, T;Läubli, H;Ramelyte, E;Fortuny, M;Landa-Magdalena, A;Moreno, I;Torres-Jiménez, J;Hernando-Calvo, A;Hess, D;Racca, F;Richly, H;Schmitt, A;Eggenschwiler, C;Sanduzzi-Zamparelli, M;Vilalta-Lacarra, A;Trajanoski, Z;Hackl, H;Gogolla, F;Koll, F;Wild, P;Chun, F;Reis, H;Lloyd, P;Machacek, M;Gajewski, T;FRIDMAN, W;Eggermont, A;Bargou, R;Schöniger, S;Rüschoff, J;Tereshchenko, A;Zink, C;Silva, A;Lichtenegger, F;Akdemir, J;Rüdiger, M;L’Huillier, P;Dutta, A;Haake, M;Auckenthaler, A;Gjorgjioska, A;Hermann, F;Rössler, B;Liebig, M;Schuberth-Wagner, C;Auer, M;Wischhusen, J;Fettes, P;Klar, K;Leo, E; | Neutralizing GDF-15 can overcome anti-PD-(L)1 resistance in late-stage cancer | Research Square | University of Navarra++START Madrid-CIOCC (Centro Integral Oncológico Clara Campal), Madrid, Spain; San Pablo CEU University, Medical Department, Madrid++Hospital 12 de Octubre++VHIO - Vall d'Hebron Institute of Oncology++Catalan Institute of Oncology (ICO) Hospitalet++Department of Medical Oncology and Hematology, Cantonal Hospital St. Gallen, Switzerland++NEXT Oncology. Phase I Unit / IOB - Hospital Quironsalud Barcelona++University Hospital Wurzburg++University Hospital Essen++University Hospital Basel++University Hospital Zurich++ICMDiM, Hospital Clínic++University of Navarra++START Madrid-CIOCC, Centro Integral Oncológico Clara Campal, Madrid, Spain++Hospital Universitario 12 de Octubre, Madrid, Spain++Vall d'Hebron Institute of Oncology (VHIO) and Hospital Universitario Vall d'Hebron++Catalan Institute of Oncology (ICO) Hospitalet++Catalan Institute of Oncology (ICO) Hospitalet++Department of Medical Oncology and Hematology, Cantonal Hospital St. Gallen, Switzerland++NEXT Oncology. Phase I Unit / IOB - Hospital Quironsalud Barcelona++University of Würzburg++West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany++University Hospital Basel++Universitätsspital Zürich++Barcelona Clinic Liver Cancer (BCLC) group. Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS); Liver Oncology Unit, Liver Unit, Hospital Clinic de Barcelona, Barcelona++Clinica Universidad de Navarra, Pamplona, Spain++START Madrid-CIOCC, Centro Integral Oncológico Clara Campal, Madrid, Spain++Hospital 12 de Octubre++Vall d'Hebron Institute of Oncology (VHIO) and Hospital Universitario Vall d'Hebron++Department of Medical Oncology and Hematology, Cantonal Hospital St. Gallen, Switzerlan++NEXT Oncology. Phase I Unit / IOB - Hospital Quironsalud Barcelona++West German Cancer Center, Department of Medical Oncology, University Hospital Essen, Essen, Germany++University Hospital Basel++Universitätsspital Zürich++Barcelona Clinic Liver Cancer (BCLC) group. Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS); Liver Oncology Unit, Liver Unit, Hospital Clinic de Barcelona, Barcelona++Department of Medical Oncology, Clinica Universidad de Navarra, Pamplona, Spain++Innsbruck Medical University++Medical University of Innsbruck++Medical University of Innsbruck, Biocenter, Institute of Bioinformatics, Innsbruck, Austria++Department of Urology, University Hospital Frankfurt, Frankfurt am Main, Germany++Dr. Senckenberg Institute of Pathology, Goethe University Frankfurt, University Hospital Frankfurt, Frankfurt am Main, Germany++Department of Urology, University Hospital Frankfurt, Frankfurt am Main, Germany++Senckenberg Institute of Pathology, University Hospital Frankfurt, Goethe University Frankfurt, Frankfurt am Main, Germany++KinDyn Consulting Ltd, Warnham, UK++LYO-X AG, Basel, Basel-Stadt, Switzerland++The University of Chicago Medical Center, Chicago, Illinois, USA++Centre de Recherche des Cordeliers++Princess Maxima Centre for Pediatric Oncology++University Hospital Wuerzburg++Discovery Life Sciences, Kassel, Germany++Discovery Life Sciences, Kassel, Germany++Discovery Life Sciences, Kassel, Germany++Metronomia GmbH, München++Da Silva Consulting Services++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++Department of Gynecology, University Hospital Würzburg, Würzburg, Germany++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried++CatalYm GmbH, Am Klopferspitz 19, 82152 Martinsried | Full Paper | |
2024 | Zourelidis, A;Trojanowicz, B;Sunami, Y;Hause, G;Vieweg, D;Kleeff, J; | Distance-depending transcriptome changes of pancreatic stellate cells in paracrine pancreatic ductal adenocarcinoma co-culture models | Research Square | Pancreatic stellate cells (PSC) represent with a percentage of 5 % of allcells, only a small part of pancreatic cells in the non-diseased pancreas [4], yet they play an | Full Paper | |
2024 | McBrearty, N;Bahal, D;Platero, S; | Fast-tracking drug development with biomarkers and companion diagnostics | Journal of Cancer Metastasis and Treatment | Discovery Life Sciences, Newtown, PA 18940, USA. #Authors contributed equally. Correspondence to: Dr. Noreen McBr | Full Paper | |
2024 | Northcott, J;Bartha, G;Harris, J;Li, C;Navarro, FCP;Pyke, RM;Hong, M;Zhang, Q;Ma, S;Chen, TX;Lai, J;Udar, N;Saldivar, JS;Ayash, E;Anderson, J;Li, J;Cui, T;Le, T;Chow, R;Velasco, RJ;Mallo, C;Santiago, R;Bruce, RC;Goodman, LJ;Chen, Y;Norton, D;Chen, RO;Lyle, JM; | Analytical Validation of NeXT Personal®, an Ultra-sensitive Personalized Circulating Tumor DNA Assay | Oncotarget | patient FFPE tumor tissue, buffy coat or adjacent normal FFPE tissue, and plasma were sourced from BOCA Biolistics (Pompano Beach, FL, USA), BioOptions (Brea, CA, USA), Cureline (Brisbane, CA, USA), Discovery Life Sciences (Huntsville, AL, USA), DxBioSamples (San Diego, CA, USA), and Us4Cure (Agoura Hills, CA, USA). Cell lines were purchased from the American Type Culture Collection and maintaine;d Blood Center (Stanford, CA). Matched sets of patient FFPE tumor tissue, buffy coat or adjacent normal FFPE tissue, and plasma were sourced from BOCA Biolistics (Pompano Beach, FL), BioOptions (Brea, CA), Cureline (Brisbane, CA), Discovery Life Sciences (Huntsville, AL), DxBioSamples (San Diego, CA), and Us4Cure (Agoura Hills, CA). Cell lines were purchased from the American Type Culture Collection and mainta | Full Paper | |
2024 | Kappell, A;Schulte, K;Scheuermann, E;Scholz, M;Keplinger, N;Scholes, A;Wolt, T;June, V;Schulte, C;Allen, L;Ternus, K;Hewitt, F; | Validation of an unbiased metagenomic detection assay for RNA viruses in viral transport media and plasma | medRxiv | Clinically diagnosed, de-identified remnant samples were obtained from Discovery Life Sciences and 366 Precision for Medicine. Of the 40 remnant samples evaluated, 9 | Full Paper | |
2024 | Kappell, A;Scholes, A;Scholz, M;Keplinger, N;Allen, L;Murray, M;Ternus, K;Hewitt, F; | Unbiased metagenomic detection of RNA viruses for rapid identification of viral pathogens in clinical samples | medRxiv | HCoV 229E contrived 75 sample was prepared by adding 149.15 µL HCoV 229E stock into 1850.85 µL SARS-CoV-2 Swab 76 Negative VTM, (Discovery Life Sciences) to | Full Paper | |
2024 | Hiatt, S;Lawlor, J;Handley, L;Latner, D;Bonnstetter, Z;Finnila, C;Thompson, M;Boston, L;Williams, M;Rodriguez Nunez, I;Jenkins, J;Kelley, W;Bebin, E;Lopez, M;Hurst, A;Korf, B;Schmutz, J;Grimwood, J;Cooper, G; | Long-read genome sequencing and variant reanalysis increase diagnostic yield in neurodevelopmental disorders | medRxiv | HudsonAlpha Institute for Biotechnology, Huntsville, AL, 35806, USA10 2Department of Neurology, University of Alabama at Birmingham, Birmingham, AL, 35924, USA11 3Department of Pediatric | Full Paper | |
2024 | Yonk, A;Linares-García, I;Pasternak, L;Juliani, S;Gradwell, M;George, A;Margolis, D; | Role of Posterior Medial Thalamus in the Modulation of Striatal Circuitry and Choice Behavior | bioRxiv | A small piece of black heat shrink tubing (Qualtek) was placed over the cannula during LED testing to prevent stray light from illuminating the presented texture during the | Full Paper | |
2024 | Le Bihan, T;Nunez de Villavicencio Diaz, T;Reitzel, C;Lange, V;Park, M;Beadle, E;Wu, L;Jovic, M;Dubois, R;Couzens, A;Duan, J;Han, X;Liu, Q;Ma, B; | de NovoSequencing of Antibodies for Identification of Neutralizing Antibodies in Human Plasma Post SARS-CoV-2 Vaccination | bioRxiv | Sample collection: Discovery Life Sciences collected three healthy donor samples; In this study, we present data specifically from one donor, labeled as "522". The | Full Paper | |
2024 | ElAbd, H;Byron, R;Woodhouse, S;Robinett, B;Sulc, J;Franke, A;Pesesky, M;Zhou, W;Chen-Harris, H;Howie, B;Taniguchi, R;Robins, H; | Seq2MAIT: A Novel Deep Learning Framework for Identifying Mucosal Associated Invariant T (MAIT) Cells | bioRxiv | nsent has been obtained for each study and the appropriate institutional forms have been archived. PBMC used for sorted repertoire experiments were collected either by DLS (Discovery Life Sciences, Huntsville, AL) under Protocol DLS13 for collection of clinical samples or by Bloodworks Northwest (Seattle, WA). Volunteer donors were consented an | Full Paper | |
2024 | Seclen, E;Jang, J;Lawal, A;Pulicani, S;Boyne, A;Tkach, D;Juillerat, A;Duchateau, P;Valton, J; | TALEN-mediated intron editing of HSPCs enables transgene expression restricted to the myeloid lineage | bioRxiv | Fresh human mobilized LeukoPaks from healthy donors were410 purchased from AllCells (Alameda, CA, USA). CD34+ HSPCs were isolated from the mobilized411 LeukoPaks using a CliniMACS Plus Instrument | Full Paper | |
2024 | Blanco, A;Coronado, R;Arun, N;Ma, K;Dar, R;Kieffer, C; | Monocyte to macrophage differentiation and changes in cellular redox homeostasis promote cell type-specific HIV latency reactivation | bioRxiv | CD14+ monocytes were magnetically labeled with CD14 microbeads (Miltenyi Biotec) and separated from PBMCs (AllCells & STEMCELL Technologies) according to the manufacturer’s instructions. The resulting cell population was stained with CD3 and CD14 antibodies (ThermoFisher Scientific) to determine purity and eva | Full Paper | |
2024 | Anaya, D;Kwong, B;Park, S;Biswas, S;Jeevan, J;Strobach, M;Khoshnoodi, N;Register, A;Klasson, T;Foos-Russ, S;Zeng, J;Banuelos, J;Gibson, C;Bravo, J;Flandez, J;Van Blarcom, T;Walker, K; | Development of Ingenui-T, a Novel Vein-to-Vein Solution for Rapid Autologous CAR T-Cell Manufacturing Starting From Whole Blood, for the Treatment of Autoimmune Diseases | bioRxiv | Donor-matched cryopreserved leukapheresis material (n=4, AllCells, USA) was also obtained to generate CAR T-cells according to a conventional manufacturing process | Full Paper | |
2024 | Daga, N;Servaas, N;Kisand, K;Moonen, D;Arnold, C;Reyes-Palomares, A;Kaleviste, E;Kingo, K;Kuuse, R;Ulst, K;Steinmetz, L;Peterson, P;Nakic, N;Zaugg, J; | Integration of genetic and epigenetic data pinpoints autoimmune specific remodelling of enhancer landscape in CD4+ T cells | bioRxiv | CD4+ T cells from healthy donors for CRISPRi experiments AllCells For CRISPRi experiments, frozen healthy donor naive CD4+ T cells were obtained from AllCells | Full Paper | |
2024 | Koh, B;Liu, M;Almonte, R;Ariad, D;Bundalian, G;Chan, J;Choi, J;Chou, W;Cuaresma, R;Hoedt, E;Hopper, L;Hu, Y;Jain, A;Khaledian, E;Khin, T;Kokate, A;Lee, J;Leung, S;Lin, C;Marispini, M;Malekpour, H;Mora, M;Mudaliar, N;Golmaei, S;Qian, H;Ramaiah, M;Ramaswamy, S;Ranjan, P;Shu, G;Spiro, P;Ta, B;Vitko, D;Waiss, J;Yanagihara, Z;Zawada, R;Zeng, J;Zhang, S;Yee, J;Blume, J;Belthangady, C;Wilcox, B;Ma, P; | Multi-omics profiling with untargeted proteomics for blood-based early detection of lung cancer | medRxiv | Blood-based approaches to detect early-stage cancer provide an opportunity to improve survival rates for lung cancer, the most lethal cancer world-wide. Multiple | Full Paper | |
2024 | Kurokawa, C;Agrawal, S;Mitra, A;Galvani, E;Burke, S;Varshine, A;Rothstein, R;Schifferli, K;Monks, N;Foloppe, J;Silvestre, N;Quemeneur, E;Demeusoit, C;Kleinpeter, P;Sapra, P;Barrett, C;Hammond, S;Kelly, E;Laliberte, J;Durham, N;Oberst, M;Broggi, M; | Mediation of antitumor activity by AZD4820 oncolytic vaccinia virus encoding IL-12 | Molecular Therapy: Oncology | Frozen DTCs were obtained from Discovery Life Sciences (Huntsville, AL) (Table S5). DTC samples were quickly thawed in a 37°C water bath and washed in 20 | Full Paper | |
2023 | Pohida, TJ;Ludington, WB;Oliver, B; | :KROH $QLPDO )HHGLQJ )/DW :$))/ D FRPSOHWH DQG FRPSUHKHQVLYH YDOLGDWLRQ RI D QRYHO KLJK WKURXJKSXW Ġ H[SHULPHQWDWLRQ VVWHP | G3 | n addition to the monitoring arena, the MUFFIN unit housed the electronics needed for the back-lighting subsystem, as well as the video acquisition and transmission: a 12V power transformer (Qualtek Electronics Corporation, London, ON) to supply the illumination PCB, 24 RPI modules which acquired video from the camera bo | Full Paper | |
2023 | Bourjaily, JL; | Computational Tools for Trees in Gauge Theory and Gravity | arXiv preprint arXiv:2312.17745 | allCells=allPositroids[8,2]; DeleteDuplicates[allCells//.positroidOrientedBoundaryRule] Out[5]= {0} | Full Paper | |
2024 | Bear, HD; | Virginia Commonwealth University Massey Cancer Center | classic.clinicaltrials.gov | Unstained FFPE slides from both core needle biopsies and the surgical resection specimen will be sent to QualTek Molecular Laboratories, the laboratory contracted by Merck for their p | Full Paper | |
2024 | Tweel, E;Oesterholt, M;van, ; | Clinical Study Protocol SYD985. 002 | classic.clinicaltrials.gov | Objectives: The primary objective of this study is:• To demonstrate that SYD985 is superior to physician's choice in prolonging progression-free survival (PFS) on the basis | Full Paper | |
2023 | Banerjee, P;Diniz, W;Dyce, SR; | Molecular Targets Differing at Weaning in Beef Heifers with Varying Reproductive Outcomes | Alabama Livestock Research Report | Libraries were sequenced in the NextSeq 500 at Discovery Life Sciences (Hudson Alpha Institute of Biotechnology, Huntsville, AL, USA). 2.4. Data Analysis | Full Paper | |
2024 | Initiative, G; | Defined Immune Response Tracking in Mice: OVA MHC Tetramers Defined Immune Response Tracking in Mice: OVA MHC Tetramers | caltagmedsystems.co.uk | ration [/abnova] * Addex Bio [/addexbio] * AdipoGen Life Sciences [/adipogen] * Affinity Biosciences [/affinity-biosciences] * Agarose Bead Technologies [/abtbeads] * AkrivisBio [/akrivisbio] * AllCells [/allcells] * Ancell [/ancell] * BeCytes Biotechnologies [/becytesbiotechnologies] * BioConcept [/bioconcept] * Biopredic [/biopredic] * Biotech Support Group [/bsg] * Bioviotica [/biov | Full Paper |
Year | Authors | Title | Journal | PubMed ID | Snippet | Link |
---|---|---|---|---|---|---|
2023 | Bellet-Ezquerra, M;Pardo, P;Fasani, R;Villacampa, G;Portu, M;Serna, G;Joval, L;Petit, A;Ortega, A;Jimenez, J;Zamora, E;Gil, M;Gomez, L;Viaplana, C;Gallego, F;Cámara, V;Manich, C;Simon, S;Nuciforo, P; | 62P KI67 in ER+HER2-negative pure invasive lobular breast carcinoma (ILC). What’s the best Ki67 threshold to differentiate prognosis? | ESMO Open | a, Novartis, Daiichi Sankyo; Non-Financial Interests, Invited Speaker: SOLTI. P.G. Nuciforo: Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, | Full Paper | |
2023 | Cieniewicz, B;Bhatta, A;Torabi, D;Baichoo, P;Saxton, M;Arballo, A;Nguyen, L;Thomas, S;Kethar, H;Kukutla, P;Shoaga, O;Yu, B;Yang, Z;Fate, M;Oliveira, E;Ning, H;Corey, L;Corey, D; | Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti-tumor responses and phagocytic uptake of tumor-associated antigen for T cell cross-presentation | Molecular therapy : the journal of the American Society of Gene Therapy | 37194236 | Healthy donor T cells were positively selected from fresh leukopak provided by AllCells (Alameda, CA) by using anti-CD4 and CD8 antibodies through the Prodigy TCT program. All related reagents and instruments were purchased from Miltenyi. After selec | Full Paper |
2023 | Overbeck, TR;Reiffert, A;Schmitz, K;Rittmeyer, A;Körber, W;Hugo, S;Schnalke, J;Lukat, L;Hugo, T;Hinterthaner, M;Reuter-Jessen, K;Schildhaus, HU; | NTRK Gene Fusions in Non-Small-Cell Lung Cancer: Real-World Screening Data of 1068 Unselected Patients | Cancers | 37296928 | r or speaker from: Amgen, AstraZeneca, Bayer, Daiichi-Sankyo, Diaceutics, Incyte, Janssen-Cilag, Eli Lilly, Merck Sharp and Dohme, Novartis Pharma, Pfizer, PharmaMar, Roche Pharma, Servier, Stemline, Targos GmbH, and ZytoVision. A.R. received grants | Full Paper |
2023 | Wagner, R;McKindles, K;Bullerjahn, G; | Effects of water movement and temperature on Rhizophydium infection of Planktothrix in a shallow hypereutrophic lake | Frontiers in Microbiology | sent to HudsonAlpha Discovery Life Sciences (Hudson, AL, United States) for sequencing. Amplicon sequencing and QC was performed by HudsonAlpha Discovery Life Save;- Discovery Life Sciences (Hudson, AL, United States) for sequencing. Amplicon se | Full Paper | |
2023 | de Vries, EGE;Rüschoff, J;Lolkema, M;Tabernero, J;Gianni, L;Voest, E;de Groot, DJA;Castellano, D;Erb, G;Naab, J;Donica, M;Deurloo, R;van der Heijden, MS;Viale, G; | Phase II study (KAMELEON) of single-agent T-DM1 in patients with HER2-positive advanced urothelial bladder cancer or pancreatic cancer/cholangiocarcinoma | Cancer medicine | 37119523 | Department of Medical Oncology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.++Targos Molecular Pathology GmbH, Kassel, Germany.++Department of Medical Oncology, Erasmus MC Cancer Institute, Rotterdam, The | Full Paper |
2023 | Wang, S;Huang, Z;Liu, Y;Sun, H;Zhou, Y;Shi, J; | Sustainably released nanoparticle-based rhynchophylline limits pulmonary fibrosis by inhibiting the TEK-PI3K/AKT signaling pathway | Translational lung cancer research | 37057119 | /tlcr-22-675/rc). Methods Solution groups For better apply to the clinic, 0.9% saline solution was used as the solvent. Because of the extremely low concentration for cell, 1 mg of Rhy (CAS 76-66-4; Allcells, Shanghai, China) was directly dissolved | Full Paper |
2023 | Naik, NN;Vadloori, B;Poosala, S;Srivastava, P;Coecke, S;Smith, A;Akhtar, A;Roper, C;Radhakrishnan, S;Bhyravbhatla, B;Damle, M;Pulla, VK;Hackethal, J;Horland, R;Li, AP;Pati, F;Singh, MS;Occhetta, P;Bisht, R;Dandekar, P;Bhagavatula, K;Pajkrt, D;Johnson, M;Weber, T;Huang, J;Hysenaj, L;Mallar, B;Ramray, B;Dixit, S;Joshi, S;Kulkarni, M; | Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Third International Conference on 3Rs Research and Progress, Vishakhapatnam, 2022 | Alternatives to laboratory animals : ATLA | 37282515 | Dr Li highlighted the discovery focus of Discovery Life Sciences LLC, namely the liver and intestine. He further described the properties of their two major novel human in Save | Full Paper |
2023 | Guler, S;DiPoto, MC;Crespo, A;Caldwell, R;Doerfel, B;Grossmann, N;Ho, K;Huck, B;Jones, CC;Lan, R;Musil, D;Potnick, J;Schilke, H;Sherer, B;Simon, S;Sirrenberg, C;Zhang, Z;Liu-Bujalski, L; | Selective Wee1 Inhibitors Led to Antitumor Activity In Vitro and Correlated with Myelosuppression | ACS medicinal chemistry letters | 37197456 | We acknowledge Bettina Hoelke, Brian Dill, Ioannis Gounaris, and Emer Clarke (ReachBio) for their technical support and discussions | Full Paper |
2023 | Tang, PZ;Ding, B;Reyes, C;Papp, D;Potter, J; | Target-seq: single workflow for detection of genome integration site, DNA translocation and off-target events | BioTechniques | 37161298 | Peripheral blood mononuclear cells (PBMCs) were purified from leukopak (ALLCELLS, CA, USA) using CTS™ Rotea™ Counterflow Centrifugation System (Thermo Fisher Scientific, MA, USA) and were then activated for 3 days to enrich the T cells (at 1 × 106 ce | Full Paper |
2023 | Singh, P; | MSC and HSPC Coculture: Mimicking Ex Vivo Bone Marrow Niche | Methods in molecular biology (Clifton, N.J.) | 36255702 | CD34+ cells with micromagnetic beads-conjugated CD34 antibody and placing with the labeled cells into a magnetic field according to the specific manual instruction. Always use fresh BM samples (within 30 h of withdrawal) for CD34+ cell isolation. Hum | Full Paper |
2023 | Simmons, KT;Chan, J;Hussain, S;Rose, EL;Markham, K;Byun, TS;Panicker, S;Parry, GC;Storek, M; | Anti-C1s humanized monoclonal antibody SAR445088: A classical pathway complement inhibitor specific for the active form of C1s | Clinical immunology (Orlando, Fla.) | 37149117 | First, 1 mL of type O- hRBCs (Allcells, Alameda, CA) was washed in 4.5 mL GVB++ by centrifuging at 600 g for 5 min. The wash step was repeated three times and the cells were resuspended to a final volume of 5 mL in GVB++ after the third wash | Full Paper |
2023 | Sikkema, L;Ramírez-Suástegui, C;Strobl, DC;Gillett, TE;Zappia, L;Madissoon, E;Markov, NS;Zaragosi, LE;Ji, Y;Ansari, M;Arguel, MJ;Apperloo, L;Banchero, M;Bécavin, C;Berg, M;Chichelnitskiy, E;Chung, MI;Collin, A;Gay, ACA;Gote-Schniering, J;Hooshiar Kashani, B;Inecik, K;Jain, M;Kapellos, TS;Kole, TM;Leroy, S;Mayr, CH;Oliver, AJ;von Papen, M;Peter, L;Taylor, CJ;Walzthoeni, T;Xu, C;Bui, LT;De Donno, C;Dony, L;Faiz, A;Guo, M;Gutierrez, AJ;Heumos, L;Huang, N;Ibarra, IL;Jackson, ND;Kadur Lakshminarasimha Murthy, P;Lotfollahi, M;Tabib, T;Talavera-López, C;Travaglini, KJ;Wilbrey-Clark, A;Worlock, KB;Yoshida, M;Lung Biological Network Consortium, ;van den Berge, M;Bossé, Y;Desai, TJ;Eickelberg, O;Kaminski, N;Krasnow, MA;Lafyatis, R;Nikolic, MZ;Powell, JE;Rajagopal, J;Rojas, M;Rozenblatt-Rosen, O;Seibold, MA;Sheppard, D;Shepherd, DP;Sin, DD;Timens, W;Tsankov, AM;Whitsett, J;Xu, Y;Banovich, NE;Barbry, P;Duong, TE;Falk, CS;Meyer, KB;Kropski, JA;Pe'er, D;Schiller, HB;Tata, PR;Schultze, JL;Teichmann, SA;Misharin, AV;Nawijn, MC;Luecken, MD;Theis, FJ; | An integrated cell atlas of the lung in health and disease | Nature medicine | 37291214 | 19-ms2/?ds=full&meta=SampleName, https://figshare.com/articles/dataset/Single-cell_RNA-Seq_of_human_primary_lung_and_bronchial_epithelium_cells/11981034/1, https://covid19.lambrechtslab.org/downloads/Allcells.counts.rds, https://s3.amazonaws.com/dp-l | Full Paper |
2023 | Sternberg, CN;Petrylak, DP;Bellmunt, J;Nishiyama, H;Necchi, A;Gurney, H;Lee, JL;van der Heijden, MS;Rosenbaum, E;Penel, N;Pang, ST;Li, JR;García Del Muro, X;Joly, F;Pápai, Z;Bao, W;Ellinghaus, P;Lu, C;Sierecki, M;Coppieters, S;Nakajima, K;Ishida, TC;Quinn, DI; | FORT-1: Phase II/III Study of Rogaratinib Versus Chemotherapy in Patients With Locally Advanced or Metastatic Urothelial Carcinoma Selected Based on FGFR1/3 mRNA Expression | Journal of clinical oncology : official journal of the American Society of Clinical Oncology | 36240478 | ted prevalence of PIK3CA and/or RAS resistance mutations, we reconfirmed absence or presence in all enrolled patients using a targeted Illumina MiSeq panel (Illumina, Inc, San Diego, CA) performed by TARGOS Molecular Pathology GmbH (Kassel, Germany). | Full Paper |
2023 | Ho, C;Fromm, J;Fang, M;Till, B;Shadman, M;Cowan, A;Lynch, R;Wu, V;Voutsinas, J;Rasmussen, H;Blue, K;Ujjani, C;Shustov, A;Cassaday, R;Gopal, A;Smith, S; | Long-term efficacy and safety of pembrolizumab with R-CHOP as first-line therapy for DLBCL. | Journal of Clinical Oncology | Additionally, prognostic factors including tumor PD-L1 expression (Qualtek/Merck) were re-analyzed. Results: 30 patients were treated (13/30 with IPI 3-5). Median follow Save | Full Paper | |
2023 | Zhang, Y;Zhao, Z;Huang, LA;Liu, Y;Yao, J;Sun, C;Li, Y;Zhang, Z;Ye, Y;Yuan, F;Nguyen, TK;Garlapati, NR;Wu, A;Egranov, SD;Caudle, AS;Sahin, AA;Lim, B;Beretta, L;Calin, GA;Yu, D;Hung, MC;Curran, MA;Rezvani, K;Gan, B;Tan, Z;Han, L;Lin, C;Yang, L; | Molecular mechanisms of snoRNA-IL-15 crosstalk in adipocyte lipolysis and NK cell rejuvenation | Cell metabolism | 37329887 | Healthy and obese human serum Coriell Biorepository See Table S1 Human breast cancer tumor tissues and paired plasma samples Discovery Life Sciences See Table S2 Primary human pre-adipocytes Zen-Bio See Table S3 | Full Paper |
2023 | Libreros, S;Nshimiyimana, R;Lee, B;Serhan, CN; | Infectious Neutrophil deployment is regulated by Resolvin D4 | Blood | 37295018 | Fresh BM aspirates from deidentified individuals were purchased from AllCells, Inc (Alameda, CA) with Mass General Brigham investigational review board protocol no. 1999P0001279. For information on donor demographics, see Supplemental Table-1. Human | Full Paper |
2023 | Dunbar, AJ;Kim, D;Lu, M;Farina, M;Bowman, RL;Yang, JL;Park, Y;Karzai, A;Xiao, W;Zaroogian, Z;O'Connor, K;Mowla, S;Gobbo, F;Verachi, P;Martelli, F;Sarli, G;Xia, L;Elmansy, N;Kleppe, M;Chen, Z;Xiao, Y;McGovern, E;Snyder, J;Krishnan, A;Hill, C;Cordner, K;Zouak, A;Salama, ME;Yohai, J;Tucker, E;Chen, J;Zhou, J;McConnell, T;Migliaccio, AR;Koche, R;Rampal, R;Fan, R;Levine, RL;Hoffman, R; | CXCL8/CXCR2 signaling mediates bone marrow fibrosis and is a therapeutic target in myelofibrosis | Blood | 36800567 | ple was independently verified by a hematopathologist (W.X.) or pulled directly from clinical pathology reports at the time of sample collection. Deidentified, healthy CD34+ cells were purchased from AllCells. CD34+ selection was carried out using Fi | Full Paper |
2023 | Kiridena, S;Wijayaratna, U;Levon, E;Moschella, P;Pirrallo, R;Tzeng, T;Anker, J; | X?Ray Visualized Sensors for Peritoneal Dialysis Catheter Infection | Advanced Functional Materials | Deidentified human peritoneal fluid was obtained from Discovery Life Sciences, Huntsville, AL, a commercial biospecimen source. Tantalum beads (0.394 mm diameter) Save | Full Paper | |
2023 | Tarafder, S;Ghataure, J;Langford, D;Brooke, R;Kim, R;Eyen, SL;Bensadoun, J;Felix, JT;Cook, JL;Lee, CH; | Advanced bioactive glue tethering Lubricin/PRG4 to promote integrated healing of avascular meniscus tears | Bioactive materials | 37214259 | ?S-encapsulating TGF?3 (R&D systems) (2.5 ?g per 500 mg PLGA) was applied to the incised tissues. Then the meniscus explants were placed on top of monolayer-cultured P2 - P3 syMSCs or hBMSCs (AllCells, Alameda, CA) at 80-90% confluence per our previo | Full Paper |
2023 | Sarno, J;Domizi, P;Liu, Y;Merchant, M;Pedersen, CB;Jedoui, D;Jager, A;Nolan, GP;Gaipa, G;Bendall, SC;Bava, FA;Davis, KL; | Dasatinib overcomes glucocorticoid resistance in B-cell acute lymphoblastic leukemia | Nature communications | 37217509 | Healthy human bone marrow samples (n = 3 for RNA-Seq, n = 4 for CyTOF) were purchased through AllCells. De-identified bone marrow samples from pediatric patients Save | Full Paper |
2023 | Yang, Y;Yang, H;Alcaina, Y;Puc, J;Birt, A;Vedvyas, Y;Gallagher, M;Alla, S;Riascos, MC;McCloskey, JE;Du, K;Gonzalez-Valdivieso, J;Min, IM;de Stanchina, E;Britz, M;von Hofe, E;Jin, MM; | Inducible expression of interleukin-12 augments the efficacy of affinity-tuned chimeric antigen receptors in murine solid tumor models | Nature communications | 37045815 | quoted and stored at ?80?°C. CAR-T cells were generated by transducing activated T cells with lentivirus. Primary T cells were enriched from commercially obtained leukopaks from healthy donors (AllCells) via MACS separation using CD4 (Miltenyi Biote | Full Paper |
2023 | Lee, JY;Jonus, HC;Sadanand, A;Branella, GM;Maximov, V;Suttapitugsakul, S;Schniederjan, MJ;Shim, J;Ho, A;Parwani, KK;Fedanov, A;Pilgrim, AA;Silva, JA;Schnepp, RW;Doering, CB;Wu, R;Spencer, HT;Goldsmith, KC; | Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastoma | Cell reports. Medicine | 37343516 | Bacterial and virus strains ------------------------- Stbl3 Competent Cells Spencer Lab N/A ------------------------- Biological samples ------------------------- Healthy PBMC AllCells LP,CR,MNC, 10M COG-424s (Neuroblastoma PDX) Chi | Full Paper |
2023 | Chen, SS;Barrientos, JC;Ferrer, G;King-Richards, M;Chen, YJ;Ravichandran, P;Ibrahim, M;Kieso, Y;Waters, S;Kutok, JL;Peluso, M;Sharma, S;Weaver, DT;Pachter, JA;Rai, KR;Chiorazzi, N; | Duvelisib Eliminates CLL B Cells, Impairs CLL-Supporting Cells, and Overcomes Ibrutinib Resistance in a Xenograft Model | Clinical cancer research : an official journal of the American Association for Cancer Research | 37071496 | Human PBMCs obtained from whole blood of CLL donors were purchased from a commercial source (Bioreclamation IVT or ALLCELLS) were resuspended (1 × 10 6 cells/ Save | Full Paper |
2023 | Xiang, J;Liu, Q;Shaknovich, R;Venn, O; | Abstract 779: Clonal B-cell expansion and the potential challenges to blood-based early cancer detection | Cancer Research | ht tubes of frozen buffy coat for a young healthy donor (18-35; no pre-existing health conditions; nonsmoker; viral negative) was sourced commercially (Discovery Life Sciences, Huntsville, AL, USA) and used as the backgrou | Full Paper | |
2023 | Fahl, S;Colwell, K;Smith, C;Ott, C;Moore, A;Williams, K; | Abstract 602: Single cell evaluation of intratumoral B cells across solid tumor indications | Cancer Research | 1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsvill | Full Paper | |
2023 | Li, A;Wei, H; | Abstract 5338: A novel in vitro assay to evaluate the roles of hepatic metabolism on anticancer drug safety and efficacy | Cancer Research | 1Discovery Life Sciences LLC, Columbia, MD.++1Discovery Life Sciences LLC, Columbia, MD | Full Paper | |
2023 | Roy, G;Kadekar, P;Douglas, L;Frappier, M;Currie, J;Dhillon, J;Cesarone, G;Siderits, R;Kirchner, K;Demeule, M;Marsolais, C; | Abstract 3942: Differential expression of a novel transport receptor, SORT1 (sortilin), in cancer versus healthy tissues that can be utilized for targeted delivery of anti-cancer drugs | Cancer Research | 1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, | Full Paper | |
2023 | Cheng, C;Chen, H;Kirchner, K;Cesarone, G; | Abstract 2763: Characterization of a novel immunohistochemistry (IHC) assay for CEACAM5 using a commercial antibody | Cancer Research | [Abcam], and 327 [Sino Biological]) to evaluate CEACAM5 detection in formalin-fixed, paraffin-embedded (FFPE) human cancer tissues from Discovery Life Sciences Save | Full Paper | |
2023 | Zielinski, D;Vogtmann, R;Siemon, A;Koppel, C;Schipper, C;Tereshchenko, A;Platero, S;Schildhaus, H; | Abstract 1018: Multiplex immunofluorescence and image analysis to investigate the role of the immune contexture and fibroblast activation for tumor cell budding in colorectal cancer | Cancer Research | 1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsvill | Full Paper | |
2023 | Kumar, A;Taghi Khani, A;Duault, C;Aramburo, S;Sanchez Ortiz, A;Lee, SJ;Chan, A;McDonald, T;Huang, M;Lacayo, NJ;Sakamoto, KM;Yu, J;Hurtz, C;Carroll, M;Tasian, SK;Ghoda, L;Marcucci, G;Gu, Z;Rosen, ST;Armenian, S;Izraeli, S;Chen, CW;Caligiuri, MA;Forman, SJ;Maecker, HT;Swaminathan, S; | Intrinsic suppression of type I interferon production underlies the therapeutic efficacy of IL-15-producing natural killer cells in B-cell acute lymphoblastic leukemia | Journal for immunotherapy of cancer | 37217248 | opoietic Tissue Biorepository and University of Pennsylvania Stem Cell and Xenograft Core after informed consent per Institutional Review Board policies. Age-matched healthy BMMCs were purchased from AllCells (Alameda, California, USA) and Stem Cell | Full Paper |
2023 | Nirschl, CJ;Brodkin, HR;Domonkos, C;Dwyer, CJ;Hicklin, DJ;Ismail, N;Seidel-Dugan, C;Steiner, P;Steuert, Z;Sullivan, JM;Winston, WM;Salmeron, A; | mWTX-330, an IL 12 INDUKINE Molecule, Activates and Reshapes Tumor-infiltrating CD8+ T and NK Cells to Generate Antitumor Immunity | Cancer immunology research | 37074216 | Dissociated human tumor samples were purchased from Discovery Life Sciences and included the following indications: bladder cancer, glioblastoma multiforme, gastric Save | Full Paper |
2023 | Diwanji, R;O'Brien, NA;Choi, JE;Nguyen, B;Laszewski, T;Grauel, AL;Yan, Z;Xu, X;Wu, J;Ruddy, DA;Piquet, M;Pelletier, MR;Savchenko, A;Charette, L;Rodrik-Outmezguine, V;Baum, J;Millholland, JM;Wong, CC;Martin, AM;Dranoff, G;Pruteanu-Malinici, I;Cremasco, V;Sabatos-Peyton, C;Jayaraman, P; | Targeting the IL1? Pathway for Cancer Immunotherapy Remodels the Tumor Microenvironment and Enhances Antitumor Immune Responses | Cancer immunology research | 37040466 | Dissociated human microsatellite-stable colorectal carcinoma (MSS-CRC) samples were obtained from Discovery Life Sciences from five donors. Between 25,000 and Save | Full Paper |
2023 | Huang, W;Lin, W;Chen, B;Zhang, J;Gao, P;Fan, Y;Lin, Y;Wei, P; | NFAT and NF-?B dynamically co-regulate TCR and CAR signaling responses in human T cells | Cell reports | 37347664 | K562 Laboratory of Wendell Lim N/A Jurkat Laboratory of Arthur Weiss N/A Human PBMC ALLCELLS PB004F-C | Full Paper |
2023 | Raj, N;Chan, JA;Wang, SJ;Aggarwal, RR;Calabrese, S;DeMore, A;Fong, L;Grabowsky, J;Hope, TA;Kolli, KP;Mulvey, CK;Munster, PN;Perez, K;Punn, S;Reidy-Lagunes, D;Von Fedak, S;Zhang, L;Bergsland, EK; | Pembrolizumab alone and pembrolizumab plus chemotherapy in previously treated, extrapulmonary poorly differentiated neuroendocrine carcinomas | British journal of cancer | 37208512 | Background To date, single-agent immune checkpoint inhibitor (CPI) therapy has proven to be ineffective against biomarker-unselected extrapulmonary poorly Save | Full Paper |
2023 | Reissig, TM;Tzianopoulos, I;Liffers, ST;Rosery, VK;Guyot, M;Ting, S;Wiesweg, M;Kasper, S;Meister, P;Herold, T;Schmidt, HH;Schumacher, B;Albers, D;Markus, P;Treckmann, J;Schuler, M;Schildhaus, HU;Siveke, JT; | Smaller panel, similar results: genomic profiling and molecularly informed therapy in pancreatic cancer | ESMO open | 37148593 | stock ownership: none; honoraries for CME presentations: Amgen, Boehringer Ingelheim, Bristol-Myers Squibb, Janssen, Novartis; research funding to institution: AstraZeneca, Bristol Myers-Squibb. HUS: Targos Molecular Pathology, Inc. (employment); Roc | Full Paper |
2023 | Han, R;Guo, H;Shi, J;Wang, H;Zhao, S;Jia, Y;Liu, X;Li, J;Cheng, L;Zhao, C;Li, X;Zhou, C; | Tumour microenvironment changes after osimertinib treatment resistance in non-small cell lung cancer | European journal of cancer (Oxford, England : 1990) | 37320935 | Fresh PBMCs of three healthy donors were purchased from Allcells (China). A total of 2 × 10 6 PBMC cells/well were seeded into 24-well plated and were treated with: Save | Full Paper |
2023 | Geoerger, B;Marshall, LV;Nysom, K;Makin, G;Bouffet, E;Defachelles, AS;Amoroso, L;Aerts, I;Leblond, P;Barahona, P;Van-Vlerken, K;Fu, E;Solca, F;Lorence, RM;Ziegler, DS; | Afatinib in paediatric patients with recurrent/refractory ErbB-dysregulated tumours: Results of a phase I/expansion trial | European journal of cancer (Oxford, England : 1990) | 37178647 | Biomarker assays were performed centrally by TARGOS Molecular Pathology GmbH using tissue samples from archived tumour blocks, or tissue sections [20]. Save | Full Paper |
2023 | Robert, ME;Rüschoff, J;Jasani, B;Graham, RP;Badve, SS;Rodriguez-Justo, M;Kodach, LL;Srivastava, A;Wang, HL;Tang, LH;Troncone, G;Rojo, F;Van Treeck, BJ;Pratt, J;Shnitsar, I;Kumar, G;Karasarides, M;Anders, RA; | Erratum to High Interobserver Variability Among Pathologists Using Combined Positive Score to Evaluate PD-L1 Expression in Gastric, Gastroesophageal Junction, and Esophageal Adenocarcinoma [Modern Pathology 36(5) (2023) 100154] | Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc | 37327723 | Yale University School of Medicine, New Haven, Connecticut. Electronic address: marie.robert@yale.edu.++Discovery Life Sciences, Hesse, Germany.++Discovery Life Sciences, Hesse, Germany.++Mayo Clinic, Rochester, Minnesota.++Emory University School of | Full Paper |
2023 | Dong, Q;Tong, M;Yu, X;Wang, L;Ao, J;Guan, D;Tang, Y;Liu, J;Long, L;Tong, Y;Fang, S;Zhou, H;Huang, Y;Gong, L;Lou, L;Huang, W; | Carbohydrate Strengthens the Immunotherapeutic Effect of Small-Molecule PD-L1 Inhibitors | Journal of medicinal chemistry | 37226718 | Stimulator cells from donor A and responder cells from donor B constitute the mixed lymphocyte reaction (MLR). Pheral Blood Mononuclear Cells (PBMCs) were purchased from Allcells (Shanghai) and used following the instruction of the manufacturer. DC c | Full Paper |
2023 | Papakonstantinou, A;Napoli, S;Joval, L;Butjosa-Espin, M;Malavila, A;Puy, M;Pimentel, I;Zamora, E;Torres, P;Rodas, N;Manich, C;Seoane, J;Nonell, L;Nuciforo, P;Oliveira, M; | 19P Tumor-associated microbiome composition and response to neoadjuvant chemotherapy (NACT) in early triple-negative breast cancer (TNBC) | ESMO Open | ncer Research (AACR). P.G. Nuciforo: Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. M. O | Full Paper | |
2023 | Akiyama, H;Carter, BZ;Andreeff, M;Ishizawa, J; | Molecular Mechanisms of Ferroptosis and Updates of Ferroptosis Studies in Cancers and Leukemia | Cells | 37190037 | Another study demonstrates that progestin and adipoQ receptor 3 (PAQR3) enhances erastin- as well as RSL3-induced ferroptosis in ALLcells through the degradation of Save | Full Paper |
2023 | Chaand, M;Fiore, C;Johnston, B;D'Ippolito, A;Moon, DH;Carulli, JP;Shearstone, JR; | Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressor | Communications biology | 37316562 | Fetal CB-derived CD34+ cells (AllCells, LLC) were transduced with concentrated lentivirus at an MOI = 1. Transduced cells were selected in the presence of 2 µg/mL Save | Full Paper |
2023 | Lin, W;Singh, V;Springer, R;Choonoo, G;Gupta, N;Patel, A;Frleta, D;Zhong, J;Owczarek, T;Decker, C;Macdonald, L;Murphy, A;Thurston, G;Mohrs, M;Ioffe, E;Lu, YF; | Human CD4 cytotoxic T lymphocytes mediate potent tumor control in humanized immune system mice | Communications biology | 37185301 | from fetal liver or cord blood, respectively. Fetal liver CD34+ HSPC were obtained from Advanced Biosciences Resources (Alameda, CA) with proper consent66 and cord blood CD34+ HSPC were obtained from AllCells, HemaCare, or STEMCELL Technologies. Huma | Full Paper |
2023 | Maden, M;Polvadore, T;Polanco, A;Barbazuk, WB;Stanley, E; | Osteoderms in a mammal the spiny mouse Acomys and the independent evolution of dermal armor | iScience | 37378333 | na NovaSeq6000 platform to yield nearly 898 million paired-end, 150 bp reads (mean of 74.8 M read pairs (s = 9.1 M read pairs) per library). Library construction and sequencing were performed by HudsonAlpha Discovery in Huntsville, Alabama, USA. Qua | Full Paper |
2023 | Egelston, CA;Guo, W;Yost, SE;Ge, X;Lee, JS;Frankel, PH;Cui, Y;Ruel, C;Schmolze, D;Murga, M;Tang, A;Martinez, N;Karimi, M;Somlo, G;Lee, PP;Waisman, JR;Yuan, Y; | Immunogenicity and efficacy of pembrolizumab and doxorubicin in a phase I trial for patients with metastatic triple-negative breast cancer | Cancer immunology, immunotherapy : CII | 37294342 | Biomarker Working Group on Breast Cancer Guidelines [18]. PD-L1 was determined by QualTek Molecular Laboratory (Goleta, CA) using immunohistochemistry (IHC) with 22C3 antibody (Merck & Co, Kenilworth, NJ). PD-L1 was positive if membrane staining was | Full Paper |
2023 | Becker, SA;Petrich, BG;Yu, B;Knight, KA;Brown, HC;Raikar, SS;Doering, CB;Spencer, HT; | Enhancing the effectiveness of ?? T cells by mRNA transfection of chimeric antigen receptors or bispecific T cell engagers | Molecular therapy oncolytics | 37387794 | ed from healthy donor blood through the Children’s Clinical Translational Discovery Core at Emory University under the core’s approved institutional review board protocol or ordered directly from AllCells. PBMCs were isolated from fresh blood using F | Full Paper |
2023 | Chervin, AS;Stone, JD;Konieczna, I;Calabrese, KM;Wang, N;Haribhai, D;Dong, F;White, MK;Rodriguez, LE;Bukofzer, GT;Ellis, PA;Cosgrove, C;Hecquet, C;Clarin, JD;Palma, JP;Reilly, EB; | ABBV-184: A novel survivin-specific TCR/CD3 bispecific T cell engager is active against both solid tumor and hematological malignancies | Molecular cancer therapeutics | 37294945 | Primary frozen viable PBMCs from AML patients and including AML blasts were purchased from Discovery Life Sciences and verified for HLA-A2 expression by staining with the HLA-A2 specific monoclonal antibody BB7.2 and detecting by flow cytometry | Full Paper |
2023 | Korntner, SH;Di Nubila, A;Gaspar, D;Zeugolis, DI; | Macromolecular crowding in animal component-free, xeno-free and foetal bovine serum media for human bone marrow mesenchymal stromal cell expansion and differentiation | Frontiers in bioengineering and biotechnology | 36949882 | ed. 2.2 Isolation and expansion of hBMSCs Fresh human whole bone marrow from the iliac crest of two different donors (donor 1: female, 22 years old; donor 2: male, 25 years old) was purchased from AllCells (United States) and hBMSCs were isolated u | Full Paper |
2023 | Dempsey, MP;Andersen, KE;Wells, BM;Taylor, MA;Cashman, CL;Conrad, LB;Kearney, CA;Conklin, MB;Via, ER;Doe, EM;Komirisetty, R;Dearborn, S;Reddy, ST;Farias-Eisner, R; | Stability Characterization of the Novel Anti-Cancer HM-10/10 HDL-Mimetic Peptide | International journal of molecular sciences | 37373203 | 82043-96OD), one plate per lot, were obtained from In Vitro ADMET Laboratories (Malden, MA, USA). The transport medium was replaced with 200 ?L of warm (37 C) Save | Full Paper |
2023 | El Ouardi, M;Tamarit, L;Vayá, I;Miranda, MA;Andreu, I; | Cellular photo(geno)toxicity of gefitinib after biotransformation | Frontiers in pharmacology | 37351506 | UPV-CSIC, Universitat Politècnica de València, Valencia, Spain 2 Unidad Mixta de Investigación UPV- IIS La Fe, Hospital Universitari i Politècnic La Fe, Valencia, Spain Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States Reviewed by | Full Paper |
2023 | Takei, S;Kinoshita, H;Jamal, M;Kumihashi, M;Yamashita, T;Tanaka, E;Kawahara, S;Abe, H;Tsutsui, K;Kimura, S; | Case report: Fatal poisoning caused by additive effects of two barbiturates | Frontiers in pharmacology | 37292155 | Medicine, Faculty of Medicine, Kagawa University, Takamatsu, Japan 2 Biodesign Inc, Tokyo, Japan 3 Kagawa Prefectural University of Health Sciences, Takamatsu, Kagawa, Japan Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States Review | Full Paper |
2023 | Li, S;Zhan, J;Wang, Y;Oduro, PK;Owusu, FB;Zhang, J;Leng, L;Li, R;Wei, S;He, J;Wang, Q; | Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery tone | Frontiers in pharmacology | 37234713 | vestigated. The EC50 and Rmax (maximal vasorelaxation) values were then calculated. 2.8 Cell culture and western blotting analysis Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells Biotech Co., Ltd. (Shanghai, China). The | Full Paper |
2023 | Greenstein, AE;Hunt, HJ; | The glucocorticoid receptor modulator relacorilant reverses the immunosuppressive effects of cortisol | International immunopharmacology | 37230031 | GR was assessed using a rabbit monoclonal antibody clone D8H2 from Cell Signaling Technology (Cat#3660S) as described previously [41]. CD3 (Dako, A0452), FOXP3 (eBioscience 14-4777), CD57 (ThermoFisher, MA5-12008), and PD-1 (Abcam, ab53587) were acqu | Full Paper |
2023 | Cyr, MG;Wilson, HD;Spierling, AL;Chang, J;Peng, H;Steinberger, P;Rader, C; | Concerted Antibody and Antigen Discovery by Differential Whole-cell Phage Display Selections and Multi-omic Target Deconvolution | Journal of molecular biology | 37019174 | Fresh healthy donor PBMCs were bought from AllCells and cryopreserved prior to use with 50% FBS, 10% DMSO in RPMI-1640 medium. Save | Full Paper |
2023 | Isaioglou, I;Aldehaiman, MM;Li, Y;Lahcen, AA;Rauf, S;Al-Amoodi, AS;Habiba, U;Alghamdi, A;Nozue, S;Habuchi, S;Salama, KN;Merzaban, JS; | CD34+ HSPCs-derived exosomes contain dynamic cargo and promote their migration through functional binding with the homing receptor E-selectin | Frontiers in cell and developmental biology | 37181754 | O2 concentration of 5% v/v. The cell cultures were passaged frequently to maintain the cells at 0.8 × 106 cells/ml. Primary human AML CD34+ cells from Mobilized Peripheral Blood were purchased from AllCells, while Primary Human CD34+ cells from Mobi | Full Paper |
2023 | Basu, A;Champagne, RN;Patel, NG;Nicholson, ED;Weiss, RJ; | TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growth | The Journal of biological chemistry | 37061003 | in Table S4. RNA sequencing and differential gene expression analysis Total RNA extracted from wildtype and knockout cell lines was submitted for library preparation and next-generation sequencing (HudsonAlpha Discovery). Raw RNA sequencing data was | Full Paper |
2023 | Spriano, F;Aresu, L;Cascione, L;Risi, G;Arribas, AJ;Napoli, S;Forster-Gross, N;Bachmann, F;Engelhardt, M;Lane, H;Bertoni, F; | The microtubule-targeted agent lisavanbulin (BAL101553) shows anti-tumor activity in lymphoma models | American journal of cancer research | 37293172 | (TMA) (LY1001D and LY2086B, TissueArray.com) were stained for EB1 using a CE-marked immunohistochemistry Clinical Trial Assay (Discovery Life Sciences Biomarker Save | Full Paper |
2023 | Baraibar, I;García, A;Salvà, F;Ros, J;Saoudi, N;Comas, R;Castillo, G;Sanchis, M;García-Álvarez, A;Hernando, J;Capdevila, J;Castells, MR;Martí, M;Landolfi, S;Espín, E;Navalpotro, B;Guevara, J;Dopazo, C;Nuciforo, P;Vivancos, A;Tabernero, J;Élez, E; | Impact of the COVID-19 pandemic in the early-onset colorectal cancer | Translational oncology | 37031602 | Paolo Nuciforo reports personal financial interest in form of scientific consultancy role for Targos Molecular Pathology. His-institution has received research funding from Save;- Paolo Nuciforo reports personal financial interest in form of scie | Full Paper |
2023 | Pousse, L;Korfi, K;Medeiros, BC;Berrera, M;Kumpesa, N;Eckmann, J;Hutter, IK;Griesser, V;Karanikas, V;Klein, C;Amann, M; | CD25 targeting with the afucosylated human IgG1 antibody RG6292 eliminates regulatory T cells and CD25+ blasts in acute myeloid leukemia | Frontiers in oncology | 37205201 | Frozen healthy BMMC were purchased from Discovery Life Sciences. All human samples were collected under the approval of the relevant Institutional Review Board (IRB Save | Full Paper |
2023 | Kourula, S;Derksen, M;Jardi, F;Jonkers, S;van Heerden, M;Verboven, P;Theuns, V;Van Asten, S;Huybrechts, T;Kunze, A;Frazer-Mendelewska, E;Lai, KW;Overmeer, R;Roos, JL;Vries, RGJ;Boj, SF;Monshouwer, M;Pourfarzad, F;Snoeys, J; | Intestinal organoids as an in vitro platform to characterize disposition, metabolism, and safety profile of small molecules | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences | 37244450 | Intestinal organoids derived from LGR5 + adult stem cells allow for long-term culturing, more closely resemble human physiology than traditional intestinal models, like Save;- HQM Incubation Medium and co-factor N were acquired from in vitro ADME | Full Paper |
2023 | Gnanapragasam, MN;Planutis, A;Glassberg, JA;Bieker, JJ; | Identification of a genomic DNA sequence that quantitatively modulates KLF1 transcription factor expression in differentiating human hematopoietic cells | Scientific reports | 37165057 | is human GRCh37/hg19 genome browser (https://main.genome-browser.bx.psu.edu/index.html), focusing on results using CD34+ or bone marrow derived erythroid cells. Human CD34+ cells were purchased from AllCells or obtained from the Yale Cooperative Cen | Full Paper |
2023 | Li, J;Zhang, Z;Zhuang, Y;Wang, F;Cai, T; | Small RNA transcriptome analysis using parallel single-cell small RNA sequencing | Scientific reports | 37160973 | ng). PBMCs preparation Cryopreserved CD3+ Pan T cells (PB009-1F-C-5M), CD19+ B cells (PB010-P-F-C), CD14+ monocytes (PB-011-P-F-1-C), and CD56+ natural killer cells (PB012-P-F-C) were purchased from AllCells (Shanghai, China). The CD3+ Pan T cells w | Full Paper |
2023 | Ke, H;Zhang, F;Wang, J;Xiong, L;An, X;Tu, X;Chen, C;Wang, Y;Mao, B;Guo, S;Ju, C;He, X;Sun, R;Zhang, L;O'Connor, OA;Li, QX; | HX009, a novel BsAb dual targeting PD1 x CD47, demonstrates potent anti-lymphoma activity in preclinical models | Scientific reports | 37012357 | Gibco). Jurkat-CSR cells, from ImmuneOnco Biopharmaceuticals (Shanghai), were cultured in RPMI-1640 (Gibco) with 10% FBS (Gemini). Human PBMCs used in the studies were purchased from ALLCELL (https://allcells.com) where informed consent and approved | Full Paper |
2023 | Adhikary, G;Heipertz, EL;Preradovic, M;Chen, X;Xu, W;Newland, JJ;Kaur, N;Vemuri, MC;Eckert, RL; | Natural killer cells suppress human cutaneous squamous cell carcinoma cancer cell survival and tumor growth | Molecular carcinogenesis | 36994661 | Cryopreserved peripheral blood mononuclear cells (PBMCs) were obtained from AllCells. NK cells were enriched via negative selection using the Human NK Cell Save | Full Paper |
2023 | Sweeney, EE;Sekhri, P;Telaraja, D;Chen, J;Chin, SJ;Chiappinelli, KB;Sanchez, CE;Bollard, CM;Cruz, CRY;Fernandes, R; | Engineered tumor-specific T cells using immunostimulatory photothermal nanoparticles | Cytotherapy | 37278683 | ix-coated plates in Astrocyte Medium (Thermo Fisher Scientific). Cells were expanded as per the specifications provided by the supplier. Healthy donor peripheral blood leukopaks were purchased from AllCells (Alameda, CA, USA) with the corresponding h | Full Paper |
2023 | Zhang, L;Yu, L;Xu, Y;Qin, P;Shen, P;Liu, K;Fei, M;Wang, H;Cao, Y;Lu, L;Gao, W;Zhang, Z; | Demonstrating analytical similarity of a biosimilar HLX04 to bevacizumab with a panel of state-of-the-art methods and tiering of quality attributes | Analytical and bioanalytical chemistry | 37162525 | Human umbilical vein endothelial cell (HUVEC), obtained from Allcells (No. H-001F, CA, USA), was used to determine the inhibitory effects of HLX04 and CN-/EU-Avastin® on proliferation of cells expressing VEGFR. HUVEC expresses VEGFR that binds to VEG | Full Paper |
2023 | Verner, EL;Jackson, JB;Severson, E;Valkenburg, KC;Greer, AE;Riley, DR;Sausen, M;Maddox, C;McGregor, PM;Karandikar, A;Hastings, SB;Previs, RA;Reddy, VP;Jensen, TJ;Ramkissoon, SH; | Validation of the Labcorp Plasma Focus Test to Facilitate Precision Oncology Through Cell-Free DNA Genomic Profiling of Solid Tumors | The Journal of molecular diagnostics : JMD | 37068734 | Clinical samples used to evaluate the analytical performance of the Labcorp Plasma Focus test were sourced from multiple biorepositories or as part of internal sample collections (BioIVT, Chestertown, MD; Discovery Life Sciences, Huntsville, AL; Prec | Full Paper |
2023 | Ding, H;Yao, B;Ci, L;Feng, J;Ouyang, P;Chen, G;Hui, X;Zhou, D; | Enhancing the Anti-tumor Potency of a Novel Siglec-15 Antibody by Engineering its Fc-mediated Effector Functions | Journal of immunotherapy (Hagerstown, Md. : 1997) | 37103472 | Total PBMCs (AllCells) from a healthy human donor were resuspended in cell culture media (RPMI1640 + 10% FBS) at a density of 150,000 cells per well in triplicate. Save | Full Paper |
2023 | Ekiciler, A;Chen, WLK;Bo, Y;Pugliano, A;Donzelli, M;Parrott, N;Umehara, K; | Quantitative Cytochrome P450 3A4 Induction Risk Assessment Using Human Hepatocytes Complemented with Pregnane X Receptor-Activating Profiles | Drug metabolism and disposition: the biological fate of chemicals | 36460477 | on 24- or 96-well collagen I-coated plates at 0.6 or 0.06 × 10 6 cells/well, respectively, using high viability cryo-hepatocyte recovery kits (In Vitro ADMET Laboratories, LLC; Save | Full Paper |
2023 | Bell-Glenn, S;Salas, LA;Molinaro, AM;Butler, RA;Christensen, BC;Kelsey, KT;Wiencke, JK;Koestler, DC; | Calculating detection limits and uncertainty of reference-based deconvolution of whole-blood DNA methylation data | Epigenomics | 37337720 | Cells used to isolate the DNA to create the mixtures were purchased from AllCells corporation (CA, USA), StemExpress (CA, USA), and STEMCELL Technologies ( Save | Full Paper |
2023 | Banerjee, P;Diniz, WJS;Rodning, SP;Dyce, PW; | miRNA expression profiles of peripheral white blood cells from beef heifers with varying reproductive potential | Frontiers in genetics | 37234872 | The quality-checked libraries were pooled and sequenced in the NextSeq 500 using the single-end 50 bp chemistry at Discovery Life Sciences (Hudson Alpha Institute of Save;nt Bioanalyzer high-sensitivity DNA assay (Agilent, Santa Clara, CA, United S | Full Paper |
2023 | Lamothe, RC;Storlie, MD;Espinosa, DA;Rudlaff, R;Browne, P;Liu, J;Rivas, A;Devoto, A;Oki, J;Khoubyari, A;Goltsman, DSA;Lin, JL;Butterfield, CN;Brown, CT;Thomas, BC;Cost, GJ; | Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome Engineering | The CRISPR journal | 37219969 | oporation, cells were harvested for flow cytometry and genomic DNA extraction as described previously. Primary HSC culture and nucleofection Mobilized peripheral blood CD34+ cells were acquired from AllCells and cultured in StemCell StemSpan SFEM I | Full Paper |
2023 | Alexander, GE;Lin, W;Ortega, FE;Ramaiah, M;Jung, B;Ji, L;Revenkova, E;Shah, P;Croisetiere, C;Berman, JR;Eubank, L;Naik, G;Brooks, J;Mich, A;Shojaee, S;Ronaghi, N;Chawla, H;Hou, X;Liu, Q;Yakym, CAV;Moradi, PW;Halks-Miller, M;Aravanis, AM;Parpart-Li, S;Hunkapiller, N; | Analytical validation of a multi-cancer early detection test with cancer signal origin using a cell-free DNA-based targeted methylation assay | PloS one | 37058491 | Six non-cancer samples were obtained from Discovery Life Sciences (Huntsville, AL; catalog #s 220130 and 220140) and 6 cancer samples were obtained from Save;ncer) across dilution series created by spiking cfDNA from cancer samples into cfDNA deriv | Full Paper |
2023 | Li, X;Le, Y;Seo, JE;Guo, X;Li, Y;Chen, S;Mittelstaedt, RA;Moore, N;Guerrero, S;Sims, A;King, ST;Atrakchi, AH;McGovern, TJ;Davis-Bruno, KL;Keire, DA;Elespuru, RK;Heflich, RH;Mei, N; | Revisiting the mutagenicity and genotoxicity of N-nitroso propranolol in bacterial and human in vitro assays | Regulatory toxicology and pharmacology : RTP | 37210026 | National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, 72079, USA. Electronic address: xilin.li@fda.hhs.gov.++National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, 72079, | Full Paper |
2023 | Coleman, R; | Metastasis prevention with bone-targeted agents: a complex interaction between the microenvironment and tumour biology | Journal of bone and mineral metabolism | 37162605 | amplification status was blinded and followed pre-specified sample handling and standard operating methodology by a central laboratory (Targos Molecular Pathology, Save | Full Paper |
2023 | Kliver, S;Houck, ML;Perelman, PL;Totikov, A;Tomarovsky, A;Dudchenko, O;Omer, AD;Colaric, Z;Weisz, D;Aiden, EL;Chan, S;Hastie, A;Komissarov, A;Ryder, OA;Graphodatsky, A;Johnson, WE;Maldonado, JE;Pukazhenthi, BS;Marinari, PE;Wildt, DE;Koepfli, KP; | Chromosome-length genome assembly and karyotype of the endangered black-footed ferret (Mustela nigripes) | The Journal of heredity | 37249392 | The black-footed ferret (Mustela nigripes) narrowly avoided extinction to become an oft-cited example of the benefits of intensive management, research, and collaboration Save | Full Paper |
2023 | Martha, L;Nakata, A;Furuya, S;Liu, W;Zhang, X;Mizoi, K;Ogihara, T; | Transporter and metabolic enzyme-mediated intra-enteric circulation of SN-38, an active metabolite of irinotecan: A new concept | Biochemical and biophysical research communications | 37148742 | sages 72-83 were used. Human cryopreserved suspension enterocytes (Lot. HE3072, male, 55 years old, Caucasian) were purchased from In Vitro ADMET Laboratories (MD, USA). All other reagents and solvents were commercial products of special grade | Full Paper |
2023 | He, S;Liu, J;Hu, L;Zhan, Y;Tong, H;Zhu, H;Guo, H;Sun, H;Liu, M; | Design, synthesis, biological evaluation and molecular docking studies of quercetin-linker-H2S donor conjugates for the treatment of diabetes and wound healing | Chemistry & biodiversity | 37329234 | All cell culture materials were obtained from Gibco. HepG2 cells were cryopreserved in our laboratory and used after resuscitation. HUVECs cells were purchased from Shanghai Allcells Co., Ltd | Full Paper |
2023 | Thompson, EN;Carlino, MJ;Scanlon, VM;Grimes, HL;Krause, DS; | Assay optimization for the objective quantification of human multilineage colony-forming units | Experimental hematology | 37271449 | Cells were derived commercially (AllCells, Alameda, CA) or were derived from healthy donors who were already donating cells for allogeneic transplantation provided Save | Full Paper |
2023 | Goda, C;Kolovich, S;Rudich, A;Karunasiri, M;Kulkarni, R;Rajgolikar, G;Neidemire-Colley, L;Singh, S;Sircar, A;Ranganathan, P;Garzon, R;Sehgal, L;Dorrance, AM; | Epidermal growth factor-like 7 is a novel therapeutic target in mantle cell lymphoma | Experimental hematology | 37209901 | Full Paper | |
2023 | Yi, PC;Zhuo, L;Lin, J;Chang, C;Goddard, A;Yoon, OK; | Impact of delayed PBMC processing on functional and genomic assays | Journal of immunological methods | 37353001 | An additional five whole blood samples from healthy adults were sourced from AllCells (Alameda, CA, USA), all of whom provided informed consent, under their IRB- Save | Full Paper |
2023 | Chen, Z;Javed, N;Moore, M;Wu, J;Sun, G;Vinyard, M;Collins, A;Pinello, L;Najm, FJ;Bernstein, BE; | Integrative dissection of gene regulatory elements at base resolution | Cell genomics | 37388913 | us Biologicals Cat#NB100-1800 TAL1 antibody SantaCruz Biotech Cat#sc-393287 Cell lines and primary cells Jurkat cell line, Clone E6.1 ATCC Cat#TIB152, RRID:CVCL_0367 CD4+ T cells AllCells N/A ------------------------- Chemical | Full Paper |
2023 | Kessler, ER;Callihan, E;Hu, J;Eule, C;Srivastava, G;Kemme, DJ;Iruku, P;Rana, V;Moore, J;Schuster, SR;Amirault, M;Flaig, TW;Lam, ET; | A Phase I/II Clinical Trial of Pembrolizumab and Cabozantinib in Metastatic Renal Cell Carcinoma | Cancer research communications | 37377613 | Full Paper | |
2023 | Farabaugh, CS;Doak, S;Roy, S;Elespuru, R; | In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin B | Frontiers in toxicology | 37180488 | Urbana?Champaign, Champaign, United States Khaled Habas, University of Bradford, United Kingdom *Correspondence: Rosalie Elespuru, rosalie.elespuru@dls.com † Present address: Rosalie Elespuru, Discovery Life Sciences, Columbia, MD, United States | Full Paper |
2023 | Baro-Sastre, B;Kim, CY;Lin, C;Kongsomboonvech, AK;Tetard, M;Salinas, ND;Tolia, NH;Egan, ES; | Plasmodium falciparum exploits CD44 as a co-receptor for erythrocyte invasion | bioRxiv : the preprint server for biology | 37090581 | Generation of cRBCs was performed essentially as previously described 37. Briefly, bone marrow primary human CD34+ HSPCs (Stem Cell Technologies; AllCells) were cultured in PIMDM composed of Iscove Basal Medium (Sigma) with 4 mM L-Glutamine, 330 µg/m | Full Paper |
2023 | Okano, F;Saito, T;Minamida, Y;Kobayashi, S;Ido, T;Miyauchi, Y;Wasai, U;Akazawa, D;Kume, M;Ishibashi, M;Jiang, K;Aicher, A;Heeschen, C;Yonehara, T; | Identification of Membrane-expressed CAPRIN-1 as a Novel and Universal Cancer Target, and Generation of a Therapeutic Anti-CAPRIN-1 Antibody TRK-950 | Cancer research communications | 37082579 | ng formula based on the Cr-51 dose released from the target cells: The ADCC activity of TRK-950 via human and monkey PBMCs was conducted at Translational Drug Development (TD2). We used human PBMCs (AllCells, catalog no. PB003F) and Cynomolgus Monke | Full Paper |
2023 | Montalban-Bravo, G;Ma, F;Thongon, N;Yang, H;Gomez, IG;Rodriguez-Sevilla, JJ;Adema, V;Wildeman, B;Lockyer, P;Kim, YJ;Tanaka, T;Darbaniyan, F;Pancholy, S;Zhang, G;Al-Atrash, G;Dwyer, K;Takahashi, K;Garcia-Manero, G;Kantarjian, H;Colla, S; | Targeting MCL1-driven anti-apoptotic pathways to overcome hypomethylating agent resistance in RAS -mutated chronic myelomonocytic leukemia | bioRxiv : the preprint server for biology | 37066354 | RAS pathway mutations, which are present in 30% of patients with chronic myelomonocytic leukemia (CMML) at diagnosis, confer a high risk of resistance to and Save | Full Paper |
2023 | Dillon, LW;Higgins, J;Nasif, H;Othus, M;Beppu, L;Smith, TH;Schmidt, E;Valentine, CC;Salk, JJ;Wood, BL;Erba, HP;Radich, JP;Hourigan, CS; | Quantification of measurable residual disease using duplex sequencing in adults with acute myeloid leukemia | medRxiv : the preprint server for health sciences | 37034683 | The presence of measurable residual disease (MRD) is strongly associated with treatment outcomes in acute myeloid leukemia (AML). Despite the correlation with clinical Save | Full Paper |
2023 | Flümann, R;Hansen, J;Pelzer, BW;Nieper, P;Lohmann, T;Kisis, I;Riet, T;Kohlhas, V;Nguyen, PH;Peifer, M;Abedpour, N;Bosco, G;Thomas, RK;Kochanek, M;Knüfer, J;Jonigkeit, L;Beleggia, F;Holzem, A;Büttner, R;Lohneis, P;Meinel, J;Ortmann, M;Persigehl, T;Hallek, M;Calado, DP;Chmielewski, M;Klein, S;Göthert, JR;Chapuy, B;Zevnik, B;Wunderlich, FT;von Tresckow, B;Jachimowicz, RD;Melnick, AM;Reinhardt, HC;Knittel, G; | Distinct Genetically Determined Origins of Myd88/BCL2-Driven Aggressive Lymphoma Rationalize Targeted Therapeutic Intervention Strategies | Blood cancer discovery | 36346827 | Roche, Cofounder and CSO of Targos MP Inc. (until May 2021), Gnothis Inc/Stockholm (Member of Board of Directors and Coowner). D.P. Calado reports other support from Cancer Research UK (CC2078), Wellcome Trust (CC2078), and UK Medical Research Counci | Full Paper |
2023 | Mark W., R;Joanne, W;Mary Jo, K;Melissa E., M;John V., L;Peter, F; | Sleep Disturbances and Chronic Pain as Potential Indicators for Therapeutic Intervention in Subjects with Post-Traumatic Stress Disorder | Journal of Depression and Anxiety Disorders | cruited to an age and gender-matched case-control study (N = 78; control n = 39; PTSD n = 39). Participants were recruited in the US between January and June 2019 by PrecisionMed (California, US) and Discovery Life Sciences (California, US). A compre | Full Paper | |
2023 | Buckley, D;Tew, B;Gooden, G;Salhia, B; | Whole genome bisulfite sequencing of tumor DNA and matching cfDNA in relapsed pediatric cancer | Research Square | from Conversant Bio (Table 1). Twelve additional cfDNA healthy controls from adults were included for model building in cfDNA, detailed below (Conversant Bio). Patients Save | Full Paper | |
2023 | Ridzuan, A;Sebastian, A;Noorazman, A;Kannan, T;Syed, N;Nurul, A; | Interleukin-17A enhances osteogenic differentiation by activating ERK/MAPK in stem cells derived from human exfoliated deciduous teeth | Biomedical Research and Therapy | the participation of the MAPK signaling pathway in SHED osteogenic differentiation. MATERIALS AND METHODS CELL CULTURE AND OSTEOGENIC DIFFERENTIATION IN VITRO The SHED cell line, obtained from ALLCELLS (Alameda, CA, USA), was derived from huma | Full Paper | |
2023 | Pillsbury, C;Dougan, J;Rabe, J;Fonseca, J;Zhou, C;Evans, A;Abukharma, H;Ichoku, O;Gonzalez-Flamenco, G;Park, S;Aljudi, A;DeRyckere, D;Castellino, S;Rafiq, S;Langermann, S;Liu, L;Henry, C;Porter, C; | Siglec-15 promotes evasion of adaptive immunity in B-cell acute lymphoblastic leukemia | Cancer Research Communications | All samples were acquired after written informed consent was provided. Residual, fresh bone marrow aspirate and peripheral blood samples from pediatric B-ALL patients were analyzed for Sig15 expression by flow cytometry under protocol (IRB#96145). Bo | Full Paper | |
2023 | Kessler, E;Callihan, E;Hu, J;Eule, C;Srivastava, G;Kemme, D;Iruku, P;Rana, V;Moore, J;Schuster, S;Amirault, M;Flaig, T;Lam, E; | Data from A Phase I/II Clinical Trial of Pembrolizumab and Cabozantinib in Metastatic Renal Cell Carcinoma | American Association for Cancer Research | PD-L1 testing was performed on archival tumor tissues by Discovery Life Sciences (formerly QualTek Molecular Laboratories) using a previously validated IHC assay for Save | Full Paper | |
2023 | Sharma, P;Zhang, X;Ly, K;Kim, J;Wan, Q;Kim, J;Lou, M;Kain, L;Teyton, L;Winau, F; | Hyperglycosylation of prosaposin in tumor DCs promotes immune escape in cancer | bioRxiv | Dissociated whole tumor samples were purchased from Discovery Life Sciences (USA). Melanoma, myeloid, and CD8 T cells were FACS-sorted as described above. Melanoma cells were ?-irradiated (10,000 rad), and induction of apoptosis was | Full Paper | |
2023 | Tierney, B;Foox, J;Ryon, K;Butler, D;Damle, N;Young, B;Mozsary, C;Babler, K;Yin, X;Carattini, Y;Andrews, D;Solle, N;Kumar, N;Shukla, B;Vidovic, D;Currall, B;Williams, S;Schürer, S;Stevenson, M;Amirali, A;Beaver, C;Kobetz, E;Boone, M;Reding, B;Laine, J;Comerford, S;Lamar, W;Tallon, J;Hirschberg, J;Proszynski, J;Sharkey, M;Church, G;Grills, G;Solo-Gabriele, H;Mason, C; | Geospatially-resolved public-health surveillance via wastewater sequencing | medRxiv | 550 HudsonAlpha Discovery (Huntsville, AL) performed all sequencing library preparation on the 551 generated nucleic acids. The nucleic acid samples underwent Save | Full Paper | |
2023 | Zhang, X;Meng, F;Lyu, W;He, J;Wei, R;Du, Z;Zhang, C;Guan, Y;Huang, X;Lyu, G;Tian, X;Zhang, L;Tao, W; | Histone lactylation antagonizes senescence and skeletal muscle aging via facilitating gene expression reprogramming | bioRxiv | IMR90 cells were purchased from the American Type Culture Collection (ATCC), HUVECs were purchased from AllCells, and MEF cells were isolated from 12.5-14- day-old embryos of C57BL/6 mice | Full Paper | |
2023 | Sorensen, JT; | Classification of a novel subgroup of shared hepatitis B virus core and e antigen epitopes and their antibody reactivity in clinical immunoassay formats | Thesis | Protocols for BSL-2 ELISA are summarized in section 5.3.1. HBV positive samples were all purchased from Discovery Life Sciences Biological Sample Repository | Full Paper | |
2023 | Najia, MA; | Transposable elements and the regulatory logic of hematopoietic differentiation | Thesis | umbilical cord blood cells (AllCells) were thawed from liquid nitrogen storage via CD34+ umbilical cord blood cells (AllCells) were thawed from liquid nitrogen storage and Save | Full Paper | |
2023 | Schejtman Borisonik, A; | Lentiviral gene therapy for p47-deficient Chronic Granulomatous disease | Thesis | CD34+ cells used in the experiments were frozen vials of previously isolated HD CD34+ cells from a G-CSF mobilised apheresis (ALLCells) using the CliniMACS prodigy | Full Paper | |
2023 | Kortchak, S; | Development of Programmed Cell Death Ligand Derived Peptides for the Treatment of Autoimmune Diseases | Thesis | Cell Culture. Jurkat NFAT-PD-1 reporter cells (BPS Biosciences, San Diego, CA) were maintained in Growth Medium 2A (BPS Biosciences, San Diego, CA) medium. Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells and maintained in RPMI | Full Paper | |
2023 | Maughon Jr, TS; | Metabolic Profiling of Mesenchymal Stromal Cells for Critical Quality Attribute Identification | Thesis | at three densities (10,000, 5,000 and 2,000 cells/well) in a 96 well plate and cultured for 24 hours. PBMCs (AllCells, Alameda CA) were thawed in Roswell Park Memorial Institute (RPMI) media (RPMI, 20% FBS, 2mM L-glutamine, 50 U/mL penicillin, 50 ?g/ | Full Paper | |
2023 | Han, L;Dong, L;Leung, K;Zhao, Z;Li, Y;Gao, L;Chen, Z;Xue, J;Qing, Y;Li, W;Pokharel, SP;Gao, M;Chen, M;Shen, C;Tan, B;Small, A;Wang, K;Zhang, Z;Qin, X;Yang, L;Wunderlich, M;Zhang, B;Mulloy, JC;Marcucci, G;Chen, CW;Wei, M;Su, R;Chen, J;Deng, X; | METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolism | Cell stem cell | 36608679 | ColonyGEL-Mouse Base Medium Reachbio Cat# 1201 ColonyGEL -Human Base Medium Reachbio Cat# 1101 | Full Paper |
2023 | Le Floc'h, A;Nagashima, K;Birchard, D;Scott, G;Ben, LH;Ajithdoss, D;Gayvert, K;Romero Hernandez, A;Herbin, O;Tay, A;Farrales, P;Korgaonkar, CK;Pan, H;Shah, S;Kamat, V;Chatterjee, I;Popke, J;Oyejide, A;Lim, WK;Kim, JH;Huang, T;Franklin, M;Olson, W;Norton, T;Perlee, L;Yancopoulos, GD;Murphy, AJ;Sleeman, MA;Orengo, JM; | Blocking common γ chain cytokine signaling ameliorates T cell-mediated pathogenesis in disease models | Science translational medicine | 36630481 | To induce GVHD, we injected immunodeficient NSG (Jackson Laboratory, 005557) female mice (8 weeks old) retro-orbitally with 10 7 human PBMCs (ReachBio, 0500-401). Mice were Save | Full Paper |
2023 | Wang, H;Georgakopoulou, A;Zhang, W;Kim, J;Gil, S;Ehrhardt, A;Lieber, A; | HDAd6/35++ - A new helper-dependent adenovirus vector platform for in vivo transduction of hematopoietic stem cells | Molecular therapy. Methods & clinical development | 37081854 | Lineage-negative (Lin-) cells were isolated by depletion of lineage-committed cells in BM MNCs using the mouse lineage cell depletion kit (Miltenyi Biotec) according to the manufacturer’s instructions. Colony-forming unit assays were performed using | Full Paper |
2023 | Belcher, JD;Nataraja, S;Abdulla, F;Zhang, P;Chen, C;Nguyen, J;Ruan, C;Singh, M;Demes, S;Olson, L;Stickens, D;Stanwix, J;Clarke, E;Huang, Y;Biddle, M;Vercellotti, GM; | The BACH1 inhibitor ASP8731 inhibits inflammation and vaso-occlusion and induces fetal hemoglobin in sickle cell disease | Frontiers in medicine | 37144034 | Department of Medicine, Division of Hematology, Oncology and Transplantation, University of Minnesota, Minneapolis, MN, United States.++Mitobridge Inc., Cambridge, MA, United States.++Department of Medicine, Division of Hematology, Oncology and Trans | Full Paper |
2023 | Wu, T;Pelus, LM;Plett, PA;Sampson, CH;Chua, HL;Fisher, A;Feng, H;Liu, L;Li, H;Ortiz, M;Chittajallu, S;Luo, Q;Bhatwadekar, AD;Meyer, TB;Zhang, X;Zhou, D;Fischer, KD;McKinzie, DL;Miller, SJ;Orschell, CM; | Further Characterization of Multi-Organ DEARE and Protection by 16,16 Dimethyl Prostaglandin E2 in a Mouse Model of the Hematopoietic Acute Radiation Syndrome | Radiation research | 37014943 | Total nucleated BM cells from non-irradiated mice were plated at 2 × 104 per dish in ColonyGEL 1202 Mouse Complete Medium (Reach Bio, Seattle, WA) | Full Paper |
2023 | Borot, F;Humbert, O;Newby, GA;Fields, E;Kohli, S;Radtke, S;Laszlo, GS;Mayuranathan, T;Ali, AM;Weiss, MJ;Yen, JS;Walter, RB;Liu, DR;Mukherjee, S;Kiem, HP; | Multiplex Base Editing to Protect from CD33-Directed Therapy: Implications for Immune and Gene Therapy | bioRxiv : the preprint server for biology | 36865281 | Cord blood CD34+ 526 cells were 527 additionally cultured with UM171 0.35 nM (Xcessbio). In colony-forming cell assays, 1000-1200 528 sorted cells were seeded into 3.5 ml ColonyGEL 1402 (ReachBio) | Full Paper |
2023 | Vujovic, A;de Rooij, L;Chahi, AK;Chen, HT;Yee, BA;Loganathan, SK;Liu, L;Chan, DCH;Tajik, A;Tsao, E;Moreira, S;Joshi, P;Xu, J;Wong, N;Balde, Z;Jahangiri, S;Zandi, S;Aigner, S;Dick, JE;Minden, MD;Schramek, D;Yeo, GW;Hope, KJ; | In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic Target | Blood cancer discovery | 36763002 | Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio cat. no. 1102), supplemented with 1.1 μmol/L DHTS (MilliporeSigma; cat. no. D0947-10MG) or equivalent DMSO (Fish | Full Paper |
2023 | Zhang, Z;Zhou, K;Han, L;Small, A;Xue, J;Huang, H;Weng, H;Su, R;Tan, B;Shen, C;Li, W;Zhao, Z;Qing, Y;Qin, X;Wang, K;Leung, K;Boldin, M;Chen, C;Ann, D;Qian, Z;Deng, X;Chen, J;Chen, Z; | RNA m6A reader YTHDF2 facilitates precursor miR-126 maturation to promote acute myeloid leukemia progression | Genes & Diseases | ColonyGel 1201-mouse base medium (1201, Reachbio) were used for seeding cells at 20, 000 per 35 mm dish. Murine cytokines and reagents were supplemented as follows: 10 ng/ Save | Full Paper | |
2023 | Davies, J;Prieto, EM;Guelcher, S; | Characterization of Macrophage Behavior in the Human Immunoresponse and Bone Remodeling | cdn.vanderbilt.edu | We cultured primary human monocytes (Reachbio, Seattle, WA) in Corning T25 flasks with 10 mL of modified Dulbecco’s modified Eagle medium (DMEM) media and split them every 9 days. We changed media on Day 1, 5, and 7, according to Table 1. Penicillin | Full Paper | |
2023 | Vujovic, A; | Investigating RNA-Binding Protein Dependencies in Hematopoietic and Acute Myeloid Leukemic Stem Cells | Thesis | Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio Cat# 1102), supplemented with 1.1μM DHTS (MilliporeSigma Cat# D0947-10MG) or equivalent DMSO (Fisher Scientific | Full Paper | |
2022 | Somarathna, M;Hwang, PT;Millican, RC;Alexander, GC;Isayeva-Waldrop, T;Sherwood, JA;Brott, BC;Falzon, I;Northrup, H;Shiu, YT;Stubben, CJ;Totenhagen, J;Jun, HW;Lee, T; | Nitric oxide releasing nanomatrix gel treatment inhibits venous intimal hyperplasia and improves vascular remodeling in a rodent arteriovenous fistula | Biomaterials | 34836683 | RNA isolation and sequencing were performed by Discovery Life Sciences (Huntsville, AL). Total RNA was isolated using standard Discovery Life Sciences protocols. All samples underwent RNA quality control assessment using a Fragment Analyzer (Agilen | Full Paper |
2022 | Choi, DY;Park, JN;Paek, SH;Choi, SC;Paek, SH; | Detecting early-stage malignant melanoma using a calcium switch-enriched exosome subpopulation containing tumor markers as a sample | Biosensors & bioelectronics | 34847362 | Clinical samples of human sera from healthy donors and malignant melanoma patients were obtained from Discovery Life Sciences (DLS; Los Osos, CA, USA). Other materials are listed and described about the sources in details in Supplementary Informati | Full Paper |
2022 | Bormann Chung, C;Lee, J;Barritault, M;Bringuier, PP;Xu, Z;Huang, WY;Beharry, A;Castillo, J;Christiansen, J;Lin, JC;Sheffield, BS; | Evaluating Targeted Next-Generation Sequencing Assays and Reference Materials for NTRK Fusion Detection | The Journal of molecular diagnostics : JMD | 34656759 | The 30 FFPE NTRK fusion-negative tumor samples (confirmed as NTRK fusion negative by an NGS laboratory-developed test) tested in the specificity analyses for AFL, TSO500, and OFA were purchased from Indivumed GmbH (Hamburg, Germany) and Discovery Lif | Full Paper |
2022 | Freeman, J;Conklin, J; | Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference material | Journal of virological methods | 34915088 | WHO 20/136 is standard reference material for SARS-CoV-2 serology assays. Standardization of serology assays that target the same antigen and class of immunoglobulin will enable comparison of results between studies that use various lab-developed a | Full Paper |
2022 | Ganan-Gomez, I;Yang, H;Ma, F;Montalban-Bravo, G;Thongon, N;Marchica, V;Richard-Carpentier, G;Chien, K;Manyam, G;Wang, F;Alfonso, A;Chen, S;Class, C;Kanagal-Shamanna, R;Ingram, JP;Ogoti, Y;Rose, A;Loghavi, S;Lockyer, P;Cambo, B;Muftuoglu, M;Schneider, S;Adema, V;McLellan, M;Garza, J;Marchesini, M;Giuliani, N;Pellegrini, M;Wang, J;Walker, J;Li, Z;Takahashi, K;Leverson, JD;Bueso-Ramos, C;Andreeff, M;Clise-Dwyer, K;Garcia-Manero, G;Colla, S; | Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapy | Nature medicine | 35241842 | simultaneously received the cytidine deaminase inhibitor E7727 (cedazuridine) to increase HMA bioavailability. BM samples from 21 HDs (median age, 53 years; range, 49-66 years) were obtained from AllCells or from MD Anderson’s Department of Stem Cel | Full Paper |
2022 | Lebedin, M;Foglierini, M;Khorkova, S;Vázquez García, C;Ratswohl, C;Davydov, AN;Turchaninova, MA;Daubenberger, C;Chudakov, DM;Lanzavecchia, A;de la Rosa, K; | Different classes of genomic inserts contribute to human antibody diversity | Proceedings of the National Academy of Sciences of the United States of America | 36037353 | 42), and ethical review boards at Ifakara Health Institute and the University of Lausanne, and all volunteers provided informed consent before blood donation. Bone marrow specimens were obtained from AllCells. Extraction of Human PBMCs. Blood sample | Full Paper |
2022 | Simmons, SK;Lithwick-Yanai, G;Adiconis, X;Oberstrass, F;Iremadze, N;Geiger-Schuller, K;Thakore, PI;Frangieh, CJ;Barad, O;Almogy, G;Rozenblatt-Rosen, O;Regev, A;Lipson, D;Levin, JZ; | Mostly natural sequencing-by-synthesis for scRNA-seq using Ultima sequencing | Nature biotechnology | 36109685 | We purchased nine cryopreserved human PMBC samples (AllCells). We thawed PBMC vials in a 37 °C water bath for ~2 min. A quick counting revealed high viability (90%) in all samples. We added 1 ml RPMI1640 (Thermo Fisher Scientific, 11875093) with 10% | Full Paper |
2022 | Shy, BR;Vykunta, VS;Ha, A;Talbot, A;Roth, TL;Nguyen, DN;Pfeifer, WG;Chen, YY;Blaeschke, F;Shifrut, E;Vedova, S;Mamedov, MR;Chung, JJ;Li, H;Yu, R;Wu, D;Wolf, J;Martin, TG;Castro, CE;Ye, L;Esensten, JH;Eyquem, J;Marson, A; | High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktails | Nature biotechnology | 36008610 | Primary adult blood cells were obtained from healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc. or Allcells Inc, as a Trima residual from Vitalant, or from fresh whole blood under a protocol approved by the UCSF C | Full Paper |
2022 | Zhang, B;Srivastava, A;Mimitou, E;Stuart, T;Raimondi, I;Hao, Y;Smibert, P;Satija, R; | Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-pro | Nature biotechnology | 35332340 | available on the protocols.io platform, here: https://www.protocols.io/private/57E45A034D5311ECA43B0A58A9FEAC02. PBMC acquisition and processing Cryopreserved healthy donor PBMCs were purchased from AllCells. After thawing into DMEM with 10% FBS, th | Full Paper |
2022 | Fiskin, E;Lareau, CA;Ludwig, LS;Eraslan, G;Liu, F;Ring, AM;Xavier, RJ;Regev, A; | Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC | Nature biotechnology | 34675424 | Thermo Fisher Scientific), resuspended in DMEM containing 10% FBS, centrifuged, washed with PBS and resuspended in FC buffer (above). For PBMC and CD8 T-cell experiments, cryopreserved PBMCs or CD8 T cells (AllCells) were thawed, washed in PBS and re | Full Paper |
2022 | Omer-Javed, A;Pedrazzani, G;Albano, L;Ghaus, S;Latroche, C;Manzi, M;Ferrari, S;Fiumara, M;Jacob, A;Vavassori, V;Nonis, A;Canarutto, D;Naldini, L; | Mobilization-based chemotherapy-free engraftment of gene-edited human hematopoietic stem cells | Cell | 35617958 | V4XP-3032 TaqMan Gene Expression Assay ThermoFisher Scientifi Cat# 4331182 ------------------------- Experimental models: Cell lines ------------------------- Human: G-CSF mPB CD34+ HSPCs AllCells N/A Human: G-CSF/Mozobil mPB CD34+ HS | Full Paper |
2022 | Battaglia, S;Dong, K;Wu, J;Chen, Z;Najm, FJ;Zhang, Y;Moore, MM;Hecht, V;Shoresh, N;Bernstein, BE; | Long-range phasing of dynamic, tissue-specific and allele-specific regulatory elements | Nature genetics | 36195755 | Epigenomic maps identify gene regulatory elements by their chromatin state. However, prevailing short-read sequencing methods cannot effectively distinguish alleles, evaluate the Save | Full Paper |
2022 | Bottomly, D;Long, N;Schultz, AR;Kurtz, SE;Tognon, CE;Johnson, K;Abel, M;Agarwal, A;Avaylon, S;Benton, E;Blucher, A;Borate, U;Braun, TP;Brown, J;Bryant, J;Burke, R;Carlos, A;Chang, BH;Cho, HJ;Christy, S;Coblentz, C;Cohen, AM;d'Almeida, A;Cook, R;Danilov, A;Dao, KT;Degnin, M;Dibb, J;Eide, CA;English, I;Hagler, S;Harrelson, H;Henson, R;Ho, H;Joshi, SK;Junio, B;Kaempf, A;Kosaka, Y;Laderas, T;Lawhead, M;Lee, H;Leonard, JT;Lin, C;Lind, EF;Liu, SQ;Lo, P;Loriaux, MM;Luty, S;Maxson, JE;Macey, T;Martinez, J;Minnier, J;Monteblanco, A;Mori, M;Morrow, Q;Nelson, D;Ramsdill, J;Rofelty, A;Rogers, A;Romine, KA;Ryabinin, P;Saultz, JN;Sampson, DA;Savage, SL;Schuff, R;Searles, R;Smith, RL;Spurgeon, SE;Sweeney, T;Swords, RT;Thapa, A;Thiel-Klare, K;Traer, E;Wagner, J;Wilmot, B;Wolf, J;Wu, G;Yates, A;Zhang, H;Cogle, CR;Collins, RH;Deininger, MW;Hourigan, CS;Jordan, CT;Lin, TL;Martinez, ME;Pallapati, RR;Pollyea, DA;Pomicter, AD;Watts, JM;Weir, SJ;Druker, BJ;McWeeney, SK;Tyner, JW; | Integrative analysis of drug response and clinical outcome in acute myeloid leukemia | Cancer cell | 35868306 | Acute myeloid leukemia (AML) is a cancer of myeloid-lineage cells with limited therapeutic options. We previously combined ex vivo drug sensitivity with genomic, transcriptomic, and Save | Full Paper |
2022 | Ma, H;Guo, Y;Tang, H;Tseng, CK;Wang, L;Zong, H;Wang, Z;He, Y;Chang, Y;Wang, S;Huang, H;Ke, Y;Yuan, Y;Wu, M;Zhang, Y;Drelich, A;Kempaiah, KR;Peng, BH;Wang, A;Yang, K;Yin, H;Liu, J;Yue, Y;Xu, W;Zhu, S;Ji, T;Zhang, X;Wang, Z;Li, G;Liu, G;Song, J;Mu, L;Xiang, Z;Song, Z;Chen, H;Bian, Y;Zhang, B;Chen, H;Zhang, J;Liao, Y;Zhang, L;Yang, L;Chen, Y;Gilly, J;Xiao, X;Han, L;Jiang, H;Xie, Y;Zhou, Q;Zhu, J; | Broad ultra-potent neutralization of SARS-CoV-2 variants by monoclonal antibodies specific to the tip of RBD | Cell discovery | 35169121 | In ADCP experiment, CD14 + monocytes (Allcells) were cultured and differentiated for 7 days to obtain macrophage cells. Macrophages were labeled with violet dye (ThermoFisher), Save | Full Paper |
2022 | Ferrari, S;Jacob, A;Cesana, D;Laugel, M;Beretta, S;Varesi, A;Unali, G;Conti, A;Canarutto, D;Albano, L;Calabria, A;Vavassori, V;Cipriani, C;Castiello, MC;Esposito, S;Brombin, C;Cugnata, F;Adjali, O;Ayuso, E;Merelli, I;Villa, A;Di Micco, R;Kajaste-Rudnitski, A;Montini, E;Penaud-Budloo, M;Naldini, L; | Choice of template delivery mitigates the genotoxic risk and adverse impact of editing in human hematopoietic stem cells | Cell stem cell | 36206730 | tain Kit Thermo Fisher Cat# L34957 7-aminoactinomycin D Sigma Aldrich SML1633; CAS: 7240-37-1 ------------------------- Biological samples ------------------------- Mobilized Leukopak AllCells N/A ------------------------- Chemica | Full Paper |
2022 | Chan, S;Belmar, N;Ho, S;Rogers, B;Stickler, M;Graham, M;Lee, E;Tran, N;Zhang, D;Gupta, P;Sho, M;MacDonough, T;Woolley, A;Kim, H;Zhang, H;Liu, W;Zheng, P;Dezso, Z;Halliwill, K;Ceccarelli, M;Rhodes, S;Thakur, A;Forsyth, CM;Xiong, M;Tan, SS;Iyer, R;Lake, M;Digiammarino, E;Zhou, L;Bigelow, L;Longenecker, K;Judge, RA;Liu, C;Trumble, M;Remis, JP;Fox, M;Cairns, B;Akamatsu, Y;Hollenbaugh, D;Harding, F;Alvarez, HM; | An anti-PD-1-GITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapy | Nature cancer | 35256819 | Human allogeneic PBMCs (AllCells) were used to purify negatively selected populations of T cells (catalog no. 19051, Stemcell) and CD11b monocytes (catalog no. 19058, Stemcell). Save | Full Paper |
2022 | Dey, M;Kim, M;Dogan, M;Nagamine, M;Kozhaya, L;Celik, N;Unutmaz, D;Ozbolat, I; | Chemotherapeutics and CAR‐T Cell‐Based Immunotherapeutics Screening on a 3D Bioprinted Vascularized Breast Tumor Model | Advanced Functional Materials | Leukopaks from healthy adults were purchased from ALLCells (Alameda, CA) in deidentified form and processed to isolate Peripheral Blood Mononuclear Cells (PBMCs) using Ficoll-paque plus (GE Healthcare). CD8+ T cells were purified from PBMCs using Dyn | Full Paper | |
2022 | Elagib, KE;Brock, A;Clementelli, CM;Mosoyan, G;Delehanty, LL;Sahu, RK;Pacheco-Benichou, A;Fruit, C;Besson, T;Morris, SW;Eto, K;Jobaliya, C;French, DL;Gadue, P;Singh, S;Shi, X;Qin, F;Cornelison, R;Li, H;Iancu-Rubin, C;Goldfarb, AN; | Relieving DYRK1A repression of MKL1 confers an adult-like phenotype to human infantile megakaryocytes | The Journal of clinical investigation | 35925681 | alculator/hypergeometric.aspx). A P value less than 0.05 was considered significant. Study approval. CD34+ cells purchased from the Fred Hutchinson Cancer Research Center (Seattle, Washington, USA), AllCells (Alameda, California, USA), and StemCell | Full Paper |
2022 | Brady, JM;Phelps, M;MacDonald, SW;Lam, EC;Nitido, A;Parsons, D;Boutros, CL;Deal, CE;Garcia-Beltran, WF;Tanno, S;Natarajan, H;Ackerman, ME;Vrbanac, VD;Balazs, AB; | Antibody-mediated prevention of vaginal HIV transmission is dictated by IgG subclass in humanized mice | Science translational medicine | 35895834 | To generate huPBMC-engrafted NSG mice, frozen huPBMCs (AllCells) were thawed and expanded in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum ( Save | Full Paper |
2022 | Jennings, D;Huntwork-Rodriguez, S;Henry, AG;Sasaki, JC;Meisner, R;Diaz, D;Solanoy, H;Wang, X;Negrou, E;Bondar, VV;Ghosh, R;Maloney, MT;Propson, NE;Zhu, Y;Maciuca, RD;Harris, L;Kay, A;LeWitt, P;King, TA;Kern, D;Ellenbogen, A;Goodman, I;Siderowf, A;Aldred, J;Omidvar, O;Masoud, ST;Davis, SS;Arguello, A;Estrada, AA;de Vicente, J;Sweeney, ZK;Astarita, G;Borin, MT;Wong, BK;Wong, H;Nguyen, H;Scearce-Levie, K;Ho, C;Troyer, MD; | Preclinical and clinical evaluation of the LRRK2 inhibitor DNL201 for Parkinson's disease | Science translational medicine | 35675433 | 2017 {study IDs: SAN-BB-01 [approved by Quorum Review independent review board (IRB)], PLMDEN201609v1 (approved by New England IRB), and US-IRB-13-2016.1 (approved by E&I Review Services)}. PBMCs from two donors for pS935 LRRK2 and pT73 Rab10 dose-re | Full Paper |
2022 | Barreyro, L;Sampson, AM;Ishikawa, C;Hueneman, KM;Choi, K;Pujato, MA;Chutipongtanate, S;Wyder, M;Haffey, WD;O'Brien, E;Wunderlich, M;Ramesh, V;Kolb, EM;Meydan, C;Neelamraju, Y;Bolanos, LC;Christie, S;Smith, MA;Niederkorn, M;Muto, T;Kesari, S;Garrett-Bakelman, FE;Bartholdy, B;Will, B;Weirauch, MT;Mulloy, JC;Gul, Z;Medlin, S;Kovall, RA;Melnick, AM;Perentesis, JP;Greis, KD;Nurmemmedov, E;Seibel, WL;Starczynowski, DT; | Blocking UBE2N abrogates oncogenic immune signaling in acute myeloid leukemia | Science translational medicine | 35263148 | BM samples directly from patients and sequenced. Healthy BM specimens were obtained from ALLCells Inc. Human CD34+ umbilical cord blood and adult BM-derived mononuclear cells were obtained from the Translational Research Development Support Laborator | Full Paper |
2022 | Sandberg, ML;Wang, X;Martin, AD;Nampe, DP;Gabrelow, GB;Li, CZ;McElvain, ME;Lee, WH;Shafaattalab, S;Martire, S;Fisher, FA;Ando, Y;Liu, E;Ju, D;Wong, LM;Xu, H;Kamb, A; | A carcinoembryonic antigen-specific cell therapy selectively targets tumor cells with HLA loss of heterozygosity in vitro and in vivo | Science translational medicine | 35235342 | Informed consent for primary T cells and donor collection protocols were approved by an Institutional Review Board at AllCells or HemaCare. Peripheral blood mononuclear cells (PBMCs) were purified from Leukopaks purchased from AllCells or HemaCare, a | Full Paper |
2022 | Uehara, K;Harumoto, T;Makino, A;Koda, Y;Iwano, J;Suzuki, Y;Tanigawa, M;Iwai, H;Asano, K;Kurihara, K;Hamaguchi, A;Kodaira, H;Atsumi, T;Yamada, Y;Tomizuka, K; | Targeted delivery to macrophages and dendritic cells by chemically modified mannose ligand-conjugated siRNA | Nucleic acids research | 35524566 | are). Preparation of human monocyte-derived macrophages and DCs Two types of human monocyte-derived macrophages (h-Mo-Mφs) were generated as described in Supplementary Figure S1A. CD14+ monocytes (AllCells, Alameda, CA, USA) were cultured (37°C, 5% | Full Paper |
2022 | Pennycuick, A;Selway, H;Lam, J;Khan, K; | 331P Predicting outcomes following colorectal cancer resection: Using real-world data to empower adjuvant treatment decision making | Annals of Oncology | : Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. A. Vivancos: Financial Interests, Perso | Full Paper | |
2022 | Glaser, M;von Levetzow, C;Michels, S;Nogova, L;Katzenmeier, M;Wömpner, C;Schmitz, J;Bitter, E;Terjung, I;Passmann, E;Schaufler, D;Eisert, A;Fischer, R;Riedel, R;Weber, J;Hahne, S;Merkelbach-Bruse, S;Büttner, R;Wolf, J;Scheffler, M; | 1729P The biological effect of small-scale ROS1 aberrations: An in silico analysis | Annals of Oncology | MS. R. Riedel: Financial Interests, Personal, Funding: Boehringer Ingelheim, Loxo Oncology, Novartis, Lilly. J. Weber: Financial Interests, Personal, Advisory Board: Lilly. R. Büttner: Financial Interests, Personal, Ownership Interest: Targos Molecul | Full Paper | |
2022 | Poole, A;Karuppiah, V;Hartt, A;Haidar, JN;Moureau, S;Dobrzycki, T;Hayes, C;Rowley, C;Dias, J;Harper, S;Barnbrook, K;Hock, M;Coles, C;Yang, W;Aleksic, M;Lin, AB;Robinson, R;Dukes, JD;Liddy, N;Van der Kamp, M;Plowman, GD;Vuidepot, A;Cole, DK;Whale, AD;Chillakuri, C; | Therapeutic high affinity T cell receptor targeting a KRASG12D cancer neoantigen | Nature communications | 36088370 | ersberg, Germany). PBMCs were either purchased cryo-frozen from commercial suppliers (Cellular Technology Limited (CTL), OH & Tissue Solutions, Glasgow, UK) or prepared from healthy donor leukopaks (ALLCELLS, CA) by Ficoll density gradient centrifug | Full Paper |
2022 | Sano, Y;Azuma, Y;Tsunenari, T;Kayukawa, Y;Shinozuka, J;Fujii, E;Amano, J;Nishito, Y;Maruyama, T;Kinoshita, Y;Sakamoto, Y;Yoshida, A;Miyazaki, Y;Sato, Y;Teramoto-Seida, C;Ishiguro, T;Tanaka, T;Kitazawa, T;Endo, M; | Combination of T cell-redirecting bispecific antibody ERY974 and chemotherapy reciprocally enhances efficacy against non-inflamed tumours | Nature communications | 36071036 | (Tokyo, Japan). After an acclimatisation of 3 weeks, mice were irradiated (2.5 Gy; MBR-1520R-3; Hitachi Power Solutions Co. Ltd., Ibaraki, Japan) 1 day before transplantation of human CD34+ cells (AllCells, Alameda, CA, USA) via intravenous injection | Full Paper |
2022 | Badrani, JH;Strohm, AN;Lacasa, L;Civello, B;Cavagnero, K;Haung, YA;Amadeo, M;Naji, LH;Lund, SJ;Leng, A;Kim, H;Baum, RE;Khorram, N;Mondal, M;Seumois, G;Pilotte, J;Vanderklish, PW;McGee, HM;Doherty, TA; | RNA-binding protein RBM3 intrinsically suppresses lung innate lymphoid cell activation and inflammation partially through CysLT1R | Nature communications | 35908044 | &D Systems, Minneapolis, MN) in T cell media. Post-stimulation supernatant was collected and stored at −80 C for ELISA analysis. Human PBMCs were isolated from commercially purchased leukopacks (Allcells, Alameda, CA, USA). Human peripheral blood IL | Full Paper |
2022 | Li, F;Zhang, H;Wang, W;Yang, P;Huang, Y;Zhang, J;Yan, Y;Wang, Y;Ding, X;Liang, J;Qi, X;Li, M;Han, P;Zhang, X;Wang, X;Cao, J;Fu, YX;Yang, X; | T cell receptor β-chain-targeting chimeric antigen receptor T cells against T cell malignancies | Nature communications | 35882880 | ded by Dr. Justin Kline (University of Chicago). B16-OVA cells were kindly provided by Dr. Hans Schreiber (University of Chicago). Human peripheral blood mononuclear cells (PBMCs) were purchased from Allcells Biotechnology, Limited (Shanghai, China) | Full Paper |
2022 | Jo, S;Das, S;Williams, A;Chretien, AS;Pagliardini, T;Le Roy, A;Fernandez, JP;Le Clerre, D;Jahangiri, B;Chion-Sotinel, I;Rozlan, S;Dessez, E;Gouble, A;Dusséaux, M;Galetto, R;Duclert, A;Marcenaro, E;Devillier, R;Olive, D;Duchateau, P;Poirot, L;Valton, J; | Endowing universal CAR T-cell with immune-evasive properties using TALEN-gene editing | Nature communications | 35773273 | cell products and to allow their large-scale utilization against multiple malignancies for the benefit of a broader range of patients. Methods Materials Cryopreserved human PBMCs were acquired from ALLCELLS (cat# PB006F). PBMCs were cultured in CTS | Full Paper |
2022 | Cai, T;Gouble, A;Black, KL;Skwarska, A;Naqvi, AS;Taylor, D;Zhao, M;Yuan, Q;Sugita, M;Zhang, Q;Galetto, R;Filipe, S;Cavazos, A;Han, L;Kuruvilla, V;Ma, H;Weng, C;Liu, CG;Liu, X;Konoplev, S;Gu, J;Tang, G;Su, X;Al-Atrash, G;Ciurea, S;Neelapu, SS;Lane, AA;Kantarjian, H;Guzman, ML;Pemmaraju, N;Smith, J;Thomas-Tikhonenko, A;Konopleva, M; | Targeting CD123 in blastic plasmacytoid dendritic cell neoplasm using allogeneic anti-CD123 CAR T cells | Nature communications | 35484100 | The production of R&D grade batches of UCART123 was done using commercially available NHPBMC cells ordered from ALLCELLS or HEMACARE. These are normal human Save | Full Paper |
2022 | Salas, LA;Zhang, Z;Koestler, DC;Butler, RA;Hansen, HM;Molinaro, AM;Wiencke, JK;Kelsey, KT;Christensen, BC; | Enhanced cell deconvolution of peripheral blood using DNA methylation for high-resolution immune profiling | Nature communications | 35140201 | lymphocytes memory (CD4mem), T regulatory cells (Treg), T-cytotoxic lymphocytes naïve (CD8nv), T-cytotoxic lymphocytes memory (CD8mem), and natural killer lymphocytes (NK) cells] were purchased from AllCells corporation (Alameda, CA, USA), StemExpre | Full Paper |
2022 | Wiesweg, M;Hense, J;Darwiche, K;Michels, S;Hautzel, H;Kobe, C;Metzenmacher, M;Herold, T;Zaun, G;Laue, K;Drzezga, A;Schildhaus, H;Wolf, J;Herrmann, K;Schuler, M; | 1171P A phase II theranostic study with osimertinib in patients with EGFR-mutated non-small cell lung cancer (NSCLC) progressing on EGFR tyrosine kinase inhibitors (TKI) and undetectable EGFR T790M (THEROS) | Annals of Oncology | ovartis Oncology; Financial Interests, Personal, Full or part-time Employment: Targos Molecular Pathology, Inc. J. Wolf: Financial Interests, Personal, Advisory Board: Amgen, AstraZeneca, Bayer, Blueprint, BMS, Boehringer Ingelheim, Chugai, Daiichi S | Full Paper | |
2022 | Chansaenroj, A;Adine, C;Charoenlappanit, S;Roytrakul, S;Sariya, L;Osathanon, T;Rungarunlert, S;Urkasemsin, G;Chaisuparat, R;Yodmuang, S;Souza, GR;Ferreira, JN; | Magnetic bioassembly platforms towards the generation of extracellular vesicles from human salivary gland functional organoids for epithelial repair | Bioactive materials | 35387159 | d EV; and (3) to identify novel signaling cues in EV that may optimize organoid development towards epithelial SG repair. 2 Materials and methods 2.1 Adult stem cell culture hDPSC were obtained from AllCells (Alameda, CA, USA) and cultured in Dulbec | Full Paper |
2022 | Kavanagh, ME;Horning, BD;Khattri, R;Roy, N;Lu, JP;Whitby, LR;Ye, E;Brannon, JC;Parker, A;Chick, JM;Eissler, CL;Wong, AJ;Rodriguez, JL;Rodiles, S;Masuda, K;Teijaro, JR;Simon, GM;Patricelli, MP;Cravatt, BF; | Selective inhibitors of JAK1 targeting an isoform-restricted allosteric cysteine | Nature chemical biology | 36097295 | In vitro TE50 values for JAK1_C817 and TYK2_C838 were obtained by treating 500 µg cell lysate generated from primary human PBMCs (AllCells), Jurkat T cells (ATCC, #TIB-152), or MDA-MB-468 breast cancer cells (ATCC, #HTB-132), with DMSO or compound fo | Full Paper |
2022 | Lam, J;Lee, B;Yu, J;Kwee, BJ;Kim, Y;Kim, J;Choi, Y;Yoon, JS;Kim, Y;Baek, K;Jeon, NL;Sung, KE; | A microphysiological system-based potency bioassay for the functional quality assessment of mesenchymal stromal cells targeting vasculogenesis | Biomaterials | 36201944 | Human bone marrow-derived MSCs were obtained from eight different donors purchased from RoosterBio (RB9, RB12, RB14, RB16; Frederick, MD) at passage 1 (P1), Lonza (8F560, 167,696; Walkersville, MD) at passage 2 (P2), and AllCells (PCBM1632, PCBM1641; | Full Paper |
2022 | Han, C;Khodadadi-Jamayran, A;Lorch, AH;Jin, Q;Serafin, V;Zhu, P;Politanska, Y;Sun, L;Gutierrez-Diaz, BT;Pryzhkova, MV;Abdala-Valencia, H;Bartom, ET;Buldini, B;Basso, G;Velu, SE;Sarma, K;Mattamana, BB;Cho, BK;Obeng, RC;Goo, YA;Jordan, PW;Tsirigos, A;Zhou, Y;Ntziachristos, P; | SF3B1 homeostasis is critical for survival and therapeutic response in T cell leukemia | Science advances | 35061527 | ing PCR to detect the TCRb-NOTCH1 translocation (TCRBJ2S4CUTLL1F: 5′-GGACCCGGCTCTCAGTGCT-3′, NOTCH1CUTTL1R: 5′-TCCCGCCCTCCAAAATAAGG-3′). Human CD3+, CD8+, and CD4+ T cells were purchased from AllCells.com (Alameda, CA) or from Astarte Biologics (Both | Full Paper |
2022 | Pan, Z;Chen, J;Xiao, X;Xie, Y;Jiang, H;Zhang, B;Lu, H;Yuan, Y;Han, L;Zhou, Y;Zong, H;Wang, L;Sun, R;Zhu, J; | Characterization of a novel bispecific antibody targeting tissue factor-positive tumors with T cell engagement | Acta pharmaceutica Sinica. B | 35847491 | for the T cell activation test were bought from AllCells (Shanghai, China). PBMC used in other experiments were isolated from leukocyte concentrate (AllCells) using Ficoll-Paque PLUS ( Save;nded conditions and used within 2 months after resuscitatio | Full Paper |
2022 | Hu, J;Zhong, Y;Shang, X; | A versatile and scalable single-cell data integration algorithm based on domain-adversarial and variational approximation | Briefings in bioinformatics | 34585247 | Integration of two modal of multimodal data on human PBMCs of a healthy female donor aged 25 that were obtained by 10x Genomics from AllCells. UMAP visualization on integrated Save | Full Paper |
2022 | Peng, S;Hu, P;Xiao, YT;Lu, W;Guo, D;Hu, S;Xie, J;Wang, M;Yu, W;Yang, J;Chen, H;Zhang, X;Zhu, Y;Wang, Y;Yang, Y;Zhu, G;Chen, S;Wang, J;Zhang, B;Chen, W;Wu, H;Sun, Z;Ding, T;Zhang, H;Yi, Z;Liu, M;Ren, S; | Single-Cell Analysis Reveals EP4 as a Target for Restoring T-Cell Infiltration and Sensitizing Prostate Cancer to Immunotherapy | Clinical cancer research : an official journal of the American Association for Cancer Research | 34740924 | Purpose: Immunotherapies targeting immune checkpoint molecules have shown promising treatment for a subset of cancers; however, many "cold" tumors, such as prostate cancer, Save | Full Paper |
2022 | Siegel, RJ;Singh, AK;Panipinto, PM;Shaikh, FS;Vinh, J;Han, SU;Kenney, HM;Schwarz, EM;Crowson, CS;Khuder, SA;Khuder, BS;Fox, DA;Ahmed, S; | Extracellular sulfatase-2 is overexpressed in rheumatoid arthritis and mediates the TNF-?-induced inflammatory activation of synovial fibroblasts | Cellular & molecular immunology | 36068294 | Human serum samples were purchased from commercial vendors (Discovery Life Sciences, Newtown, PA; Innovative Research, Novi, MI; or Sanguine Biosciences, Los Angeles, CA) or generously shared by Dr. Cynthia Crowson (Mayo Clinic, Rochester, MN) | Full Paper |
2022 | Gyurdieva, A;Zajic, S;Chang, YF;Houseman, EA;Zhong, S;Kim, J;Nathenson, M;Faitg, T;Woessner, M;Turner, DC;Hasan, AN;Glod, J;Kaplan, RN;D'Angelo, SP;Araujo, DM;Chow, WA;Druta, M;Demetri, GD;Van Tine, BA;Grupp, SA;Fine, GD;Eleftheriadou, I; | Biomarker correlates with response to NY-ESO-1 TCR T cells in patients with synovial sarcoma | Nature communications | 36075914 | s were digitally obtained using CaseViewer (3D Histech, Budapest, Hungary). Protein expression by IHC NY-ESO-1 staining was performed using the E978 clone (Sigma, Catalog #: N2038, at 1??g/mL) at QualTek Laboratory (Goleta, CA; now part of Discovery | Full Paper |
2022 | Ni, Z;Sun, P;Zheng, J;Wu, M;Yang, C;Cheng, M;Yin, M;Cui, C;Wang, G;Yuan, L;Gao, Q;Li, Y; | JNK Signaling Promotes Bladder Cancer Immune Escape by Regulating METTL3-Mediated m6A Modification of PD-L1 mRNA | Cancer research | 35502544 | CD8+ T cells (catalog no. PB009-3F-C) were obtained from AllCells (Shanghai) and cultured in ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies, catalog no. 10981). Seventy-two hours after activation with ImmunoCult Human CD3/CD28/CD2 T Cel | Full Paper |
2022 | Ge, X;Yost, SE;Lee, JS;Frankel, PH;Ruel, C;Cui, Y;Murga, M;Tang, A;Martinez, N;Chung, S;Yeon, C;Stewart, D;Li, D;Rajurkar, S;Somlo, G;Mortimer, J;Waisman, J;Yuan, Y; | Phase II Study Combining Pembrolizumab with Aromatase Inhibitor in Patients with Metastatic Hormone Receptor Positive Breast Cancer | Cancers | 36077811 | mal TILs were measured by hematoxylin and eosin (H&E) staining of formalin-fixed paraffin-embedded (FFPE) tumor biopsies according to the International TIL Work Group guideline [16]. PD-L1 analysis: QualTek Molecular Laboratory (Goleta, CA, USA) per | Full Paper |
2022 | Shirk, B;Shirk, P;Furlong, R;Scully, E;Wu, K;Siegfried, B; | Gene Editing of the ABC Transporter/White Locus Using Crispr/Cas9-Mediated Mutagenesis in the Indian Meal Moth | SSRN Electronic Journal | y and sequencing, and Kris Hartzer for laboratory assistance. Library 497 preparation and sequencing were performed at HudsonAlpha Genomic Services Lab 498 (Huntsville, AL). We also thank Karen Garren for her work to isolate the P. interpunctella 499 | Full Paper | |
2022 | Kanter, J;Thompson, AA;Pierciey, FJ;Hsieh, M;Uchida, N;Leboulch, P;Schmidt, M;Bonner, M;Guo, R;Miller, A;Ribeil, JA;Davidson, D;Asmal, M;Walters, MC;Tisdale, JF; | Lovo-cel gene therapy for sickle cell disease: Treatment process evolution and outcomes in the initial groups of the HGB-206 study | American journal of hematology | 36161320 | MCW served as medical director for AllCells and as a consultant for AllCells, Ensoma, Vertex, and BioLabs. The remaining authors declare no competing financial interests. Save | Full Paper |
2022 | Wei, Y;Kanagal-Shamanna, R;Zheng, H;Bao, N;Lockyer, PP;Class, CA;Darbaniyan, F;Lu, Y;Lin, K;Yang, H;Montalban-Bravo, G;Ganan-Gomez, I;Soltysiak, KA;Do, KA;Colla, S;Garcia-Manero, G; | Cooperation between KDM6B overexpression and TET2 deficiency in the pathogenesis of chronic myelomonocytic leukemia | Leukemia | 35697791 | Materials and methods Human samples BM specimens were obtained from the MD Anderson Cancer Center tissue bank following institutional guidelines. BM samples from healthy individuals were obtained from AllCells (Emeryville, CA) | Full Paper |
2022 | Lu, M;Xia, L;Elmansy, N;Clementelli, C;Tremblay, D;Hoffman, R; | Combined Drug Targeting of p53-dependent and -independent Pathways Depletes Myelofibrosis Hematopoietic Stem/Progenitor Cells | Leukemia | 34642468 | met the World Health Organization criteria for the diagnosis of MF [41, 42]. Patient characteristics are provided in Supplemental Table 1. Normal donor (ND) human bone marrow (BM) was purchased from AllCells (Emeryville, CA). The experiments in which | Full Paper |
2022 | Bae, J;Accardi, F;Hideshima, T;Tai, YT;Prabhala, R;Shambley, A;Wen, K;Rowell, S;Richardson, PG;Munshi, NC;Anderson, KC; | Targeting LAG3/GAL-3 to overcome immunosuppression and enhance anti-tumor immune responses in multiple myeloma | Leukemia | 34290359 | roval by the Institutional Review Board at Dana-Farber Cancer Institute (Boston, MA). In addition, healthy individuals BM [N = 5] or leukapheresis [N = 12] products were purchased from either AllCells (Alameda, CA) or the Blood Donor Center at Boston | Full Paper |
2022 | Huang, G;Cheng, Z;Hildebrand, A;Wang, C;Cimini, M;Roy, R;Lucchese, AM;Benedict, C;Mallaredy, V;Magadum, A;Joladarashi, D;Thej, C;Gonzalez, C;Trungcao, M;Garikipati, VNS;Elrod, JW;Koch, WJ;Kishore, R; | Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibition | Theranostics | 35673580 | days, db/+ EPCs were cultured at 37°C, 5% CO2 atmosphere. Male mice were used to avoid the reproductive hormone effect in female mice. Human CD34+ Hematopoietic stem cells (HSC) were purchased from AllCells, LLC and were cultured in a human hematopoi | Full Paper |
2022 | Shen, Y;Eng, JS;Fajardo, F;Liang, L;Li, C;Collins, P;Tedesco, D;Nolan-Stevaux, O; | Cancer cell-intrinsic resistance to BiTE therapy is mediated by loss of CD58 costimulation and modulation of the extrinsic apoptotic pathway | Journal for immunotherapy of cancer | 35296559 | Target cells were cocultured with freshly thawed human CD3 + T cells (ALLCELLS) and increasing concentrations of TCEs. Assay conditions were effector-to-target ratio (E:T)=2:1 and Save | Full Paper |
2022 | Froning, KJ;Sereno, A;Huang, F;Demarest, SJ; | Generalizable design parameters for soluble T cell receptor-based T cell engagers | Journal for immunotherapy of cancer | 35260435 | ously11 with minor modifications throughout the protocol. Saos-2 and HCT-116 cells were cultured as described earlier. Primary naïve T cells were from StemExpress (cat#PB03020C, Donor#D001003581) or AllCells (cat# PB009-1F, Lt# 3009286). For the assa | Full Paper |
2022 | Wu, ZH;Li, N;Mei, XF;Chen, J;Wang, XZ;Guo, TT;Chen, G;Nie, L;Chen, Y;Jiang, MZ;Wang, JT;Wang, HB; | Preclinical characterization of the novel anti-SIRPα antibody BR105 that targets the myeloid immune checkpoint | Journal for immunotherapy of cancer | 35256517 | Allogeneic PBMCs (AllCells) from different donors were added at a dendritic cell:PBMCs ratio of 1:20. Indicated mAbs were added at a concentration of 10 µg/mL immediately, and the Save | Full Paper |
2022 | Lee, KM;Lin, CC;Servetto, A;Bae, J;Kandagatla, V;Ye, D;Kim, G;Sudhan, DR;Mendiratta, S;González Ericsson, PI;Balko, JM;Lee, J;Barnes, S;Malladi, VS;Tabrizi, S;Reddy, SM;Yum, S;Chang, CW;Hutchinson, KE;Yost, SE;Yuan, Y;Chen, ZJ;Fu, YX;Hanker, AB;Arteaga, CL; | Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer | Cancer immunology research | 35561311 | After a week, 1 A 107 human PBMCs (hPBMC; ALLCELLS) were injected intravenously. Two weeks later, SUM159PT tumors were harvested for analysis of tumor-infiltrating immune Save | Full Paper |
2022 | Li, W;Yang, S;Xu, P;Zhang, D;Tong, Y;Chen, L;Jia, B;Li, A;Lian, C;Ru, D;Zhang, B;Liu, M;Chen, C;Fu, W;Yuan, S;Gu, C;Wang, L;Li, W;Liang, Y;Yang, Z;Ren, X;Wang, S;Zhang, X;Song, Y;Xie, Y;Lu, H;Xu, J;Wang, H;Yu, W; | SARS-CoV-2 RNA elements share human sequence identity and upregulate hyaluronan via NamiRNA-enhancer network | EBioMedicine | 35124429 | Human umbilical vein endothelial cells (HUVEC) were cultured in the commercial culture medium (AllCells, Cat# H-004). VERO C1008 (E6) (Cat# GNO17) and its culture medium MEM Save;ed with 10% FBS (Gibco, Cat# 10270-106) and 1% Penicillin-Streptomyci | Full Paper |
2022 | Zhang, C;Chen, Z;Liu, J;Wu, M;Yang, J;Zhu, Y;Lu, W;Ruan, C; | 3D-printed pre-tapped-hole scaffolds facilitate one-step surgery of predictable alveolar bone augmentation and simultaneous dental implantation | Composites Part B: Engineering | The human bone marrow mesenchymal stem cells (hBMSCs, obtained from Allcells, Silicon Valley, CA) were cultured in α-MEM (Gibco, USA) supplemented with 1% penicillin-streptomycin (Gibco, USA) and 10% fetal bovine serum (FBS) (Gibco, USA) in an atmosp | Full Paper | |
2022 | Petaroudi, M;Rodrigo-Navarro, A;Dobre, O;Dalby, MJ;Salmeron-Sanchez, M; | Living Biomaterials to Engineer Hematopoietic Stem Cell Niches | Advanced healthcare materials | 35933595 | Fresh, cryopreserved bone marrow CD34+ cells isolated from the posterior iliac crest of healthy and consenting donors were purchased from AllCells. All cultures containing CD34+ Save | Full Paper |
2022 | Suyama, T;Takemoto, Y;Miyauchi, H;Kato, Y;Matsuzaki, Y;Kato, R; | Morphology-based noninvasive early prediction of serial-passage potency enhances the selection of clone-derived high-potency cell bank from mesenchymal stem cells | Inflammation and regeneration | 36182958 | Bone marrow mononuclear cells were prepared from bone marrow aspirate (AllCells, Alameda, USA) collected from healthy donors using density gradient centrifugation with Ficoll (GE Save Cached | Full Paper |
2022 | Wang, J;Jiang, J;Yang, X;Zhou, G;Wang, L;Xiao, B; | Tethering Piezo channels to the actin cytoskeleton for mechanogating via the cadherin-β-catenin mechanotransduction complex | Cell reports | 35139384 | Experimental models: Cell lines HEK293T Xiao et al., 2011 N/A MDCK Xu Tan Lab in Tsinghua University N/A HUVEC Purchased from Allcells N/A | Full Paper |
2022 | Sun, Y;Wang, T;Lv, Y;Li, J;Jiang, X;Jiang, J;Zhang, D;Bian, W;Zhang, C; | MALAT1 promotes platelet activity and thrombus formation through PI3k/Akt/GSK-3β signalling pathway | Stroke and vascular neurology | 36241224 | Allcells Biotechnology (Shanghai, China) was the supplier of the Cord Blood-Stem/Progenitor (CD34+) cells that were used in this study. Serum-free expansion medium (StemCell Technologies, Canada) was used in the cultivation of CD34+ cells. To facilit | Full Paper |
2022 | Manshouri, T;Veletic, I;Li, P;Yin, CC;Post, SM;Verstovsek, S;Estrov, Z; | GLI1 activates pro-fibrotic pathways in myelofibrosis fibrocytes | Cell death & disease | 35595725 | ld Health Organization revised criteria [1]. Control BM biopsies were obtained from morphologically normal BM donors and control BM aspirates were obtained from commercially available healthy donors (AllCells, Alameda, CA, USA). The patients’ and con | Full Paper |
2022 | Zhang, J;Liu, F;Yang, Y;Yu, N;Weng, X;Yang, Y;Gong, Z;Huang, S;Gan, L;Sun, S;Zhang, X;Gong, Y;Liu, Y;Guo, W; | Integrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancer | Cell death & disease | 35449126 | ze the results using the positive control contained in the different membranes. T-cell amplification and tumor-reactive T cell killing assay Peripheral blood mononuclear cells (PBMCs) purchased from AllCells (China) were activated with anti-CD3 anti | Full Paper |
2022 | Liu, P;Zhang, Q;Mi, J;Wang, S;Xu, Q;Zhuang, D;Chen, W;Liu, C;Zhang, L;Guo, J;Wu, X; | Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246 | Stem cell research & therapy | 35241153 | Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Cat. H-001F, AllCells) and were cultured in DMEM (Cat. SH30021.01, Hyclone) with 4.5 g/L D-Glucose Save | Full Paper |
2022 | McLemore, AF;Hou, HA;Meyer, BS;Lam, NB;Ward, GA;Aldrich, AL;Rodrigues, MA;Vedder, A;Zhang, L;Padron, E;Vincelette, ND;Sallman, DA;Abdel-Wahab, O;List, AF;McGraw, KL; | Somatic gene mutations expose cytoplasmic DNA to co-opt the cGAS/STING/NLRP3 axis in myelodysplastic syndromes | JCI insight | 35788117 | using Ficoll-Paque (GE Healthcare) from patients with MDS and healthy donors who provided informed consent at the Moffitt Cancer Center or NTUH or were purchased from AllCells. Save Cached | Full Paper |
2022 | Xie, J;Gui, X;Deng, M;Chen, H;Chen, Y;Liu, X;Ku, Z;Tan, L;Huang, R;He, Y;Zhang, B;Lewis, C;Chen, K;Xu, L;Xu, J;Huang, T;Liao, XC;Zhang, N;An, Z;Zhang, CC; | Blocking LAIR1 signaling in immune cells inhibits tumor development | Frontiers in immunology | 36211388 | Human allogeneic pan T cells (PB009-1F, Allcells) were stained with Carboxy fluorescein succinimidyl ester (CFSE) (C34554, Thermo Fisher Scientific), and mixed with 3 x10 5 mature Save | Full Paper |
2022 | Hu, JF;Wang, ZW;Liao, CY;Chen, ZW;Kang, FP;Lin, CF;Lin, TS;Huang, L;Tian, YF;Chen, S; | Induced expression of CCL19 promotes the anti-tumor ability of CAR-T cells by increasing their infiltration ability | Frontiers in immunology | 35990619 | (CRL-1682, ATCC) cells were obtained from ATCC and cultured in RPMI-1640 medium (cat. no. 11875093; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. Human T cells were obtained from Allcells (cat. no. PB009-CD3-F). Both of the cells | Full Paper |
2022 | Bjornson-Hooper, ZB;Fragiadakis, GK;Spitzer, MH;Chen, H;Madhireddy, D;Hu, K;Lundsten, K;McIlwain, DR;Nolan, GP; | A Comprehensive Atlas of Immunological Differences Between Humans, Mice, and Non-Human Primates | Frontiers in immunology | 35359965 | apeutics between humans and model organisms will greatly improve the evaluation of therapeutic candidates. Materials and Methods Blood Venous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human sub | Full Paper |
2022 | Peng, H;Nerreter, T;Mestermann, K;Wachter, J;Chang, J;Hudecek, M;Rader, C; | ROR1-targeting switchable CAR-T cells for cancer therapy | Oncogene | 35859167 | Pan-T cells were isolated from fresh PBMC of healthy donors (Allcells) by negative selection (Pan T Cell Isolation Kit; Miltenyi Biotec), activated using Dynabeads Human T-Activator Save | Full Paper |
2022 | Zhang, M;Zhu, Y;Zhu, J;Xie, Y;Wu, R;Zhong, J;Qiu, Z;Jiang, L; | circ_0086296 induced atherosclerotic lesions via the IFIT1/STAT1 feedback loop by sponging miR-576-3p | Cellular & molecular biology letters | 36138395 | HUVECs were obtained from AllCells (Shanghai, China) as previously reported [7, 33]. HUVECs were seeded in EC complete growth medium at 37 C. Then, 100 μg/mL ox-LDL was Save Cached | Full Paper |
2022 | Abdul-Aziz, A;Weigel, C;Kovacs, A;Wu, Y;Byrd, J;Hertlein, E;Oakes, C; | P381: DNA METHYLATION PROFILING OF MESENCHYMAL STROMAL CELLS ISOLATED FROM FEMURAL HEAD BONE MARROW VERSUS BONE MARROW ASPIRATES: RELEVANCE FOR AML STUDY BASED CONTROLS | HemaSphere | Aspirate-derived BMSCs (n= 7) were expanded from bone marrow mononuclear cells obtained from a commercial source (AllCells). For genome-wide DNA methylation analyses, the Save | Full Paper | |
2022 | Owens, TD;Brameld, KA;Verner, EJ;Ton, T;Li, X;Zhu, J;Masjedizadeh, MR;Bradshaw, JM;Hill, RJ;Tam, D;Bisconte, A;Kim, EO;Francesco, M;Xing, Y;Shu, J;Karr, D;LaStant, J;Finkle, D;Loewenstein, N;Haberstock-Debic, H;Taylor, MJ;Nunn, P;Langrish, CL;Goldstein, DM; | Discovery of Reversible Covalent Bruton's Tyrosine Kinase Inhibitors PRN473 and PRN1008 (Rilzabrutinib) | Journal of medicinal chemistry | 35302767 | The ability of selected compounds to block BCR-driven activation of B cells was assessed by expression of the CD69 surface marker, as previously described. (37) Briefly, HWB (AllCells, Alameda, CA, USA) pretreated for 1 h with serial dilutions of tes | Full Paper |
2022 | Zheng, L;Wang, H;Zuo, P;Liu, Y;Xu, H;Ye, BC; | Rapid On-Chip Isolation of Cancer-Associated Exosomes and Combined Analysis of Exosomes and Exosomal Proteins | Analytical chemistry | 35575685 | The biotin-labeled rabbit antihuman CD63 antibody (anti-CD63) was purchased from AllCells Biotech Co., Ltd. (Shanghai, China). The horseradish peroxidase (HRP)-conjugated secondary antibody, rabbit anti-human CD9 polyclonal antibody, and bicinchonini | Full Paper |
2022 | Avanzino, BC;Prabhakar, K;Dalvi, P;Hartstein, S;Kehm, H;Balasubramani, A;Boudreau, AA;Buelow, B;Chang, K;Davison, LM;Iyer, S;Kalwit, V;Lewis Wilson, K;Malik-Chaudhry, HK;Pierson, W;Pineda, G;Rangaswamy, US;Saiganesh, S;Schellenberger, U;Ugamraj, HS;Yabut, RD;Buelow, R;Chapman, J;Trinklein, ND;Harris, KE; | A T-cell engaging bispecific antibody with a tumor-selective bivalent folate receptor alpha binding arm for the treatment of ovarian cancer | Oncoimmunology | 36016696 | In a similar follow-on study, NCG mice were injected with activated human PBMCs (AllCells). Prior to injection, PBMCs were activated for 3 d with 1 µg/mL plate-coated anti-CD3 ( Save | Full Paper |
2022 | Xue, W;Zhang, Q;Chen, Y;Zhu, Y; | Hydrogen Sulfide Improves Angiogenesis by Regulating the Transcription of pri-miR-126 in Diabetic Endothelial Cells | Cells | 36078059 | The HUVECs from passages 4 to 7 were used in the experiment and were cultured in endothelial completed medium (ALLCELLS, Shanghai, China) at 37 C in 5% CO 2 . The HUVECs Save Cached | Full Paper |
2022 | Sharma, M;Bellio, MA;Benny, M;Kulandavelu, S;Chen, P;Janjindamai, C;Han, C;Chang, L;Sterling, S;Williams, K;Damianos, A;Batlahally, S;Kelly, K;Aguilar-Caballero, D;Zambrano, R;Chen, S;Huang, J;Wu, S;Hare, JM;Schmidt, A;Khan, A;Young, K; | Mesenchymal Stem Cell-derived Extracellular Vesicles Prevent Experimental Bronchopulmonary Dysplasia Complicated By Pulmonary Hypertension | Stem cells translational medicine | 35758326 | es, and isotype control was performed on P2 WJ-MSC to confirm the MSC identity. BM-MSC Production BM-MSCs were isolated from fresh adult bone marrow aspirates, and obtained from a commercial source (AllCells, Alameda, CA). Briefly, bone marrow was p | Full Paper |
2022 | Ohori-Morita, Y;Niibe, K;Limraksasin, P;Nattasit, P;Miao, X;Yamada, M;Mabuchi, Y;Matsuzaki, Y;Egusa, H; | Novel Mesenchymal Stem Cell Spheroids with Enhanced Stem Cell Characteristics and Bone Regeneration Ability | Stem cells translational medicine | 35267026 | heir therapeutic potential in bone regeneration. Materials and Methods BM-MSC Adherent Culture Human BM-MSCs of an 18-year-old male (donor 1; Lonza, Basel, Switzerland), a 22-year-old male (donor 2; AllCells, Alameda, CA, USA), and a 24-year-old mal | Full Paper |
2022 | Stratmann, S;Yones, SA;Garbulowski, M;Sun, J;Skaftason, A;Mayrhofer, M;Norgren, N;Herlin, MK;Sundström, C;Eriksson, A;Höglund, M;Palle, J;Abrahamsson, J;Jahnukainen, K;Munthe-Kaas, MC;Zeller, B;Tamm, KP;Cavelier, L;Komorowski, J;Holmfeldt, L; | Transcriptomic analysis reveals proinflammatory signatures associated with acute myeloid leukemia progression | Blood advances | 34619772 | children (Table 1). Detailed biological characteristics and supporting clinical information are reported in supplemental Tables 2 and 3. CD34-expressing bone marrow (BM) cells from 5 healthy donors (AllCells Inc., Alameda, CA) were used as normal con | Full Paper |
2022 | Jeon, E;Choi, D;Choi, S;Won, J;Jo, Y;Kim, H;Jung, Y;Shin, S;Min, H;Choi, H;Lee, M;Park, Y;Chung, J;Jin, H; | Enhancing adoptive T‐cell therapy with fucoidan‐based IL ‐2 delivery microcapsules | Bioengineering & Translational Medicine | Cryopreserved human peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryvill, CA). Total CD3+T cells were isolated by negative selection with a magnetic isolation kit and CD4+T, CD8+T, and regulatory T (Treg) cells were then | Full Paper | |
2022 | Lun, Y;Zhou, Y;Li, Q;Chen, P;Huang, Y;Ye, G; | Preparation and characterization of a magnetic microsphere synthesized from sucrose allyl ether for transcatheter arterial embolization | Materials Today Chemistry | Human umbilical vein endothelial cells (HUVECs, AllCells, US) were subcultured in a low glucose Dulbecco's modified eagle medium containing 10% fetal bovine serum and 1% penicillin/streptomycin (100 U·mL−1) in an atmosphere of 310.15 K and 5% CO2 | Full Paper | |
2022 | Lu, W;Zhou, C;Ma, Y;Li, J;Jiang, J;Chen, Y;Dong, L;He, F; | Improved osseointegration of strontium-modified titanium implants by regulating angiogenesis and macrophage polarization | Biomaterials science | 35384947 | HUVECs together with complete and basal medium were purchased from Allcells Bio (Shanghai, China). The murine macrophage cell lines (Raw264.7) were obtained from the Save | Full Paper |
2022 | Zhou, Y;Li, XH;Xue, WL;Jin, S;Li, MY;Zhang, CC;Yu, B;Zhu, L;Liang, K;Chen, Y;Tao, BB;Zhu, YZ;Wang, MJ;Zhu, YC; | YB-1 Recruits Drosha to Promote Splicing of pri-miR-192 to Mediate the Proangiogenic Effects of H2S | Antioxidants & redox signaling | 35044231 | HUVECs were purchased from Allcells (Shanghai, China) and cultured in endothelial basal medium (EBM; Allcells, Shanghai, China) supplemented with hydrocortisone, bovine brain Save | Full Paper |
2022 | Li, J;Hong, T;Wei, Y;Guo, L;Lee, M;Yang, H;Class, C;Yang, Y;Wang, X;He, H;Siwko, S;You, MJ;Zhou, Y;Garcia-Manero, G;Huang, Y; | Aberrant DNA hydroxymethylation reshapes transcription factor binding in myeloid neoplasms | Clinical epigenetics | 35765052 | BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). BM mononuclear cells were isolated using a Ficoll-Paque density gradient media (GE, Sweden). Save Cached | Full Paper |
2022 | Fang, J;Li, R;Zhang, Y;Oduro, PK;Li, S;Leng, L;Wang, Z;Rao, Y;Niu, L;Wu, HH;Wang, Q; | Aristolone in Nardostachys jatamansi DC. induces mesenteric vasodilation and ameliorates hypertension via activation of the KATP channel and PDK1-Akt-eNOS pathway | Phytomedicine : international journal of phytotherapy and phytopharmacology | 35738117 | Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells Biotech Co. Ltd. (Shanghai, China) and cultured in Endothelial Cell Medium (ECM; AllCells Biotech Co. Ltd., Shanghai, China) with 10% fetal bovine serum, 1% endothelial growt | Full Paper |
2022 | Liang, W;Chen, J;Zheng, H;Lin, A;Li, J;Wu, W;Jie, Q; | MiR-199a-5p-containing macrophage-derived extracellular vesicles inhibit SMARCA4 and alleviate atherosclerosis by reducing endothelial cell pyroptosis | Cell biology and toxicology | 35930100 | Human monocyte-derived macrophages (hMDMs, PB-MDM-001F, AllCells) were purchased from Milestone Biotechnologies (Shanghai, China). The hMDMs were cultured at 37 °C in RPMI1640 medium (supplemented with 10% FBS and 1% penicillin/streptomycin) with 5% | Full Paper |
2022 | Wang, J;Li, C;He, K;Kuang, Z;Lu, J;Yao, Y;He, F;Li, N;Li, L;Fu, F;Wu, Z;Zhou, S;Kang, D;Qiu, X;Wu, M;Liu, Y;Cao, X;Xu, M;Chen, B;Wu, W;Guo, F; | Characterization of anti-CD79b/CD3 bispecific antibody, a potential therapy for B cell malignancies | Cancer immunology, immunotherapy : CII | 35963895 | Materials and methods Reagents IBI38D9 and human IgG1 were produced by Innovent Biologics Co., Ltd. (Suzhou, China). Human IgG was purchased from Equitech-Bio. Peripheral blood mononuclear cells (PBMCs) were purchased from the AllCells | Full Paper |
2022 | Ni, H;Cao, L;Wu, Z;Wang, L;Zhou, S;Guo, X;Gao, Y;Jing, H;Wu, M;Liu, Y;Ding, J;Zhang, P;Zhou, Y;Chen, B;Xiong, Y;Sun, J;Prinz, B;Baruah, H;Geoghegan, J;Yu, M;Wu, W;Liu, J; | Combined strategies for effective cancer immunotherapy with a novel anti-CD47 monoclonal antibody | Cancer immunology, immunotherapy : CII | 34165607 | CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMCs, Allcells) and then differentiated into macrophages following incubation for 7 days with granulocyte- Save | Full Paper |
2022 | Wu, ZH;Li, N;Gao, ZZ;Chen, G;Nie, L;Zhou, YQ;Jiang, MZ;Chen, Y;Chen, J;Mei, XF;Hu, F;Wang, HB; | Development of the Novel Bifunctional Fusion Protein BR102 That Simultaneously Targets PD-L1 and TGF-β for Anticancer Immunotherapy | Cancers | 36230887 | IgG1 isotype control was expressed by Biointron Biological (Taizhou, China). 2.2. Cell Lines and Cell Culture Dendritic cells (DCs) and peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Shanghai, China). TF-1 cells were from t | Full Paper |
2022 | Wang, X;Chen, G;Nie, L;Wu, Z;Wang, X;Pan, C;Chen, X;Zhao, X;Zhu, J;He, Q;Wang, H; | IL-2K35C-moFA, a Long-Acting Engineered Cytokine with Decreased Interleukin 2 Receptor α Binding, Improved the Cellular Selectivity Profile and Antitumor Efficacy in a Mouse Tumor Model | Cancers | 36230665 | Next, 100 μL CellTiter-Glo (G7572, Promega Corporation, Madison, WI, USA) was added; luminescence was recorded suing a Spectramax M5. 2.8. p-STAT5 Assay in Treg and CD8+ T Cell Tregs (PB009-4F-2, Allcells, Shanghai, China) at a density of 1 × 106 c | Full Paper |
2022 | Sekhri, P;Ledezma, DK;Shukla, A;Sweeney, EE;Fernandes, R; | The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma Cells | Cancers | 35326601 | Healthy donor peripheral blood leukopaks were purchased from AllCells (Alameda, CA, USA) along with the HLA report. Peripheral blood mononuclear cells (PBMCs) were isolated by Save | Full Paper |
2022 | Ning, K;Roy, A;Cheng, F;Xu, P;Kleiboeker, S;Escalante, CR;Wang, J;Qiu, J; | High-Throughput Screening Identifies Inhibitors for Parvovirus B19 Infection of Human Erythroid Progenitors | Journal of virology | 34669461 | Primary human CD34+ hematopoietic stem cells (HSCs), which were isolated from the bone marrow of a healthy human donor, were purchased from AllCells LLC (Alameda, CA). B19V-containing plasma samples were obtained from ViraCor Eurofins Laboratories (L | Full Paper |
2022 | Liu, Y;Tsang, K;Mays, M;Hansen, G;Chiecko, J;Crames, M;Wei, Y;Zhou, W;Fredrick, C;Hu, J;Liu, D;Gebhard, D;Huang, ZF;Datar, A;Kronkaitis, A;Gueneva-Boucheva, K;Seeliger, D;Han, F;Sen, S;Kasturirangan, S;Scheer, JM;Nixon, AE;Panavas, T;Marlow, MS;Kumar, S; | An adapted consensus protein design strategy for identifying globally optimal biotherapeutics | mAbs | 35613320 | isolated from human peripheral blood mononuclear cells (PBMCs) using a negative selection kit (EasySep). PBMCs were isolated from Leukopak collected from healthy volunteers, which were obtained from ALLcells. Redirected T-cell cytotoxicity was assaye | Full Paper |
2022 | Zhao, J;Jiang, L;Yang, H;Deng, L;Meng, X;Ding, J;Yang, S;Zhao, L;Xu, W;Wang, X;Zhu, Z;Huang, H; | A strategy for the efficient construction of anti-PD1-based bispecific antibodies with desired IgG-like properties | mAbs | 35239451 | #F9512) at 4°C for 1 h. Cells were washed three times and then analyzed on the CytoFLEX Cytometer System (Beckman Coulter). Allogeneic mixed lymphocyte reaction Monocytes were enriched from PBMCs (AllCells, Cat# PB002-C-300) by adherence on tissue c | Full Paper |
2022 | McGuire, BE;Mela, JE;Thompson, VC;Cucksey, LR;Stevens, CE;McWhinnie, RL;Winkler, DFH;Pelech, S;Nano, FE; | Escherichia coli recombinant expression of SARS-CoV-2 protein fragments | Microbial cell factories | 35123472 | mall number of serum or plasma samples from positive tested donors and all pre-COVID-19 samples were acquired by purchase (Precision for Medicine, Norton, MA, USA; Innovative Research, Novi, MI, USA; AllCells, Alameda, CA, USA) or by donation (CureIm | Full Paper |
2022 | Li, G;Reid, KM;Spitler, K;Beatty, N;Boucher, J;Davila, ML; | CD3 engagement as a new strategy for allogeneic "off-the-shelf" T cell therapy | Molecular therapy oncolytics | 35317526 | lls was harvested, and 0.45 μm was filtered and cryopreserved for future use. For T cell transduction, healthy donor leukopaks were purchased from Stemcell Technologies (Vancouver, BC, Canada) and ALLCELLS (Alameda, CA). PBMCs were isolated using sta | Full Paper |
2022 | Biscaia, S;Branquinho, MV;Alvites, RD;Fonseca, R;Sousa, AC;Pedrosa, SS;Caseiro, AR;Guedes, F;Patrício, T;Viana, T;Mateus, A;Maurício, AC;Alves, N; | 3D Printed Poly(????-caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on a Composite Preparation by Melt Blending or Solvent Casting Techniques and the Influence of Bioceramic Content on Scaffold Properties | International journal of molecular sciences | 35216432 | ON 4505 equipment in a dry state at a rate of 1 mm/min. Five specimens were tested for each composition. 4.3. Cytocompatibility Analysis 4.3.1. Cell Culture and Maintenance hDPSCs were acquired from AllCells, LLC (Cat. DP0037F, Lot N° DPSC090411-01) | Full Paper |
2022 | Cunningham, AW;Jones, M;Frank, N;Sethi, D;Miller, MM; | Stem-like memory T cells are generated during hollow fiber perfusion-based expansion and enriched after cryopreservation in an automated modular cell therapy manufacturing process | Cytotherapy | 36031522 | Leukopaks from three healthy donors were acquired by processing whole blood on the Spectra Optia Apheresis System and obtained through AllCells (Alameda, CA, USA) (Fig. 1A). The apheresis product was shipped to the lab overnight, and CD3+ T cells wer | Full Paper |
2022 | Jimenez, AC;Heist, CA;Navaei, M;Yeago, C;Roy, K; | Longitudinal two-dimensional gas chromatography mass spectrometry as a non-destructive at-line monitoring tool during cell manufacturing identifies volatile features correlative to cell product quality | Cytotherapy | 35882596 | CellBIND 96-well plate (Corning) for 48-h before co-culture at 50 000 cells per well. PBMCs (AllCells, Alameda, CA, USA) were thawed with a CTL anti-aggregate solution (Cellular Technology Ltd, Shaker Heights, OH, USA) and plated in a six-well plate | Full Paper |
2022 | Maughon, TS;Shen, X;Huang, D;Michael, AOA;Shockey, WA;Andrews, SH;McRae, JM;Platt, MO;Fernández, FM;Edison, AS;Stice, SL;Marklein, RA; | Metabolomics and cytokine profiling of mesenchymal stromal cells identify markers predictive of T-cell suppression | Cytotherapy | 34696960 | ethylenediaminetetraacetic acid; Gibco) and then seeded at three densities (10 000, 5000 and 2000 cells/well) in a 96-well plate and cultured for 24 h. PBMCs (AllCells, Alameda, CA, USA) were thawed in Roswell Park Memorial Institute medium, 20% feta | Full Paper |
2022 | Xu, C;Yang, J;Kosters, A;Babcock, BR;Qiu, P;Ghosn, EEB; | Comprehensive multi-omics single-cell data integration reveals greater heterogeneity in the human immune system | iScience | 36185375 | ical samples ------------------------- Human Adult Peripheral Blood Emory University’s Children’s Clinical and Translational Discovery Core N/A Human Adult Bone Marrow Emory University; AllCells N/A ------------------------- Critical co | Full Paper |
2022 | Magis, W;DeWitt, MA;Wyman, SK;Vu, JT;Heo, SJ;Shao, SJ;Hennig, F;Romero, ZG;Campo-Fernandez, B;Said, S;McNeill, MS;Rettig, GR;Sun, Y;Wang, Y;Behlke, MA;Kohn, DB;Boffelli, D;Walters, MC;Corn, JE;Martin, DIK; | High-level correction of the sickle mutation is amplified in vivo during erythroid differentiation | iScience | 35633935 | Cryopreserved CD34+ cells were thawed according to AllCells instructions and cultured for 2 days in StemSpan SFEM with CC110 supplement (StemCell Technologies). For each Save;. Methods CD34+cells CD34+ HSPCs homozygous for the sickle mutation were | Full Paper |
2022 | Liu, P;Qin, L;Liu, C;Mi, J;Zhang, Q;Wang, S;Zhuang, D;Xu, Q;Chen, W;Guo, J;Wu, X; | Exosomes Derived From Hypoxia-Conditioned Stem Cells of Human Deciduous Exfoliated Teeth Enhance Angiogenesis via the Transfer of let-7f-5p and miR-210-3p | Frontiers in cell and developmental biology | 35557954 | Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Cat. H-001F, AllCells) and were cultured in DMEM (Cat. SH30021.01, Hyclone) supplemented with 10 Save | Full Paper |
2022 | Li, L;Zhou, B;Xu, H;Shi, H;Gao, L;Ge, B; | Zinc-Loaded Black Phosphorus Multifunctional Nanodelivery System Combined With Photothermal Therapy Have the Potential to Treat Prostate Cancer Patients Infected With COVID-19 | Frontiers in endocrinology | 35464050 | n, USA). Human HMGB1 ELISA Kit was obtained from Arigo Biolaboratories (Taiwan, China). ATP content detection kit was obtained from Solarbio (Beijing, China). Dendritic cells (DCs) were obtained from AllCells (San Francisco, USA). FITC anti-human CD1 | Full Paper |
2022 | McGovern, K;Castro, AC;Cavanaugh, J;Coma, S;Walsh, M;Tchaicha, J;Syed, S;Natarajan, P;Manfredi, M;Zhang, XM;Ecsedy, J; | Discovery and Characterization of a Novel Aryl Hydrocarbon Receptor Inhibitor, IK-175, and Its Inhibitory Activity on Tumor Immune Suppression | Molecular cancer therapeutics | 35666806 | Naive human T cells (CD4+/CD62L+) from two independent donors were isolated from cryopreserved CD4+ T cells (AllCells) by magnetic selection with human CD62 L microbeads (Miltenyi). On Day 1, naive CD62L+ human T cells were activated with human CD3/C | Full Paper |
2022 | Lin, W;Qian, J;Wang, H;Ren, L;Yang, Y;Chen, C;Chen, X;Huang, Y;Liu, J;Xu, N;Teng, L; | Cisplatin plus anti-PD-1 antibody enhanced treatment efficacy in advanced esophageal squamous cell carcinoma | American journal of cancer research | 35261780 | Therapies for patients with advanced esophageal squamous cell carcinoma (ESCC) are limited and accompanied by dismal prognosis. Here we use ESCC cell line K30 and TE-1 to Save | Full Paper |
2022 | Duan, J;Lei, D;Ling, C;Wang, Y;Cao, Z;Zhang, M;Zhang, H;You, Z;Yao, Q; | Three-dimensional-printed polycaprolactone scaffolds with interconnected hollow-pipe structures for enhanced bone regeneration | Regenerative biomaterials | 35719204 | CR MasterMix (ABM, CA) on a Light Cycler 480 II (Roche, CHE). To evaluate the expression of angiogenesis genes (HIF1-α and VEGF), human umbilical vein endothelial cells (HUVECs) were purchased from AllCells. First, 6 × 105 HUVECs were seeded vertica | Full Paper |
2022 | Li, Z;Kou, L;Fu, X;Xie, Z;Xu, M;Guo, L;Lin, T;Gong, S;Zhang, S;Liu, M; | Design, synthesis, and biological evaluation of triazole-pyrimidine-methylbenzonitrile derivatives as dual A2A/A2B adenosine receptor antagonists | Journal of enzyme inhibition and medicinal chemistry | 35616298 | Cryopreserved PBMCs (Allcells) were thawed and recovered in RPMI1640 medium overnight before being used. And compounds to be tested were diluted with DMSO to 100 folds of Save | Full Paper |
2022 | Hu, S;Muniraj, G;Mishra, A;Hong, K;Lum, JL;Hong, CHL;Rosa, V;Sriram, G; | Characterization of silver diamine fluoride cytotoxicity using microfluidic tooth-on-a-chip and gingival equivalents | Dental materials : official publication of the Academy of Dental Materials | 35778310 | Objective This study aims to characterize the cytotoxicity potential of silver diamine fluoride (SDF) on dental pulp stem cells (DPSC) and gingival equivalents. Methods DPSC cultured on Save | Full Paper |
2022 | Dogan, M;Karhan, E;Kozhaya, L;Placek, L;Chen, X;Yigit, M;Unutmaz, D; | Engineering Human MAIT Cells with Chimeric Antigen Receptors for Cancer Immunotherapy | Journal of immunology (Baltimore, Md. : 1950) | 36165183 | Healthy adult blood was obtained from AllCells (Quincy, MA). PBMCs were isolated using Ficoll-Paque plus (GE Healthcare). CD4 + T, CD8 + T, and CD19 + B cells and CD14 + Save | Full Paper |
2022 | Ben Nasr, M;Robbins, D;Parone, P;Usuelli, V;Tacke, R;Seelam, AJ;Driver, E;Le, T;Sabouri-Ghomi, M;Guerrettaz, L;Shoemaker, D;Fiorina, P; | Pharmacologically Enhanced Regulatory Hematopoietic Stem Cells Revert Experimental Autoimmune Diabetes and Mitigate Other Autoimmune Disorders | Journal of immunology (Baltimore, Md. : 1950) | 35321879 | A total of 5000 cryopreserved and preisolated human mobilized peripheral blood HSCs (CD34 + cells) (AllCells) were plated into each well of a 384-well plate (Corning) in StemSpan- Save | Full Paper |
2022 | Melani, RD;Des Soye, BJ;Kafader, JO;Forte, E;Hollas, M;Blagojevic, V;Negrão, F;McGee, JP;Drown, B;Lloyd-Jones, C;Seckler, HS;Camarillo, JM;Compton, PD;LeDuc, RD;Early, B;Fellers, RT;Cho, BK;Mattamana, BB;Goo, YA;Thomas, PM;Ash, MK;Bhimalli, PP;Al-Harthi, L;Sha, BE;Schneider, JR;Kelleher, NL; | Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire | Journal of proteome research | 34878788 | odium heparin (BD 367874, Fisher Scientific) by centrifugation at 1,500 × g for 10 min. Plasma from a convalescent patient featuring a high titer of anti-SARS-CoV-2-RBD antibodies was purchased from AllCells (commercial sample 1 - CS1) and used as a | Full Paper |
2022 | Kawato, Y;Fukahori, H;Nakamura, K;Kanno, A;Kubo, K;Hiramitsu, M;Matsuda, T;Hanada, Y;Furukawa, T;Nakajima, Y;Kinugasa, F;Morokata, T; | Potential benefit of the cathepsin S inhibitor, ASP1617, as a treatment for systemic lupus erythematosus | European journal of pharmacology | 35157914 | Five different lots of peripheral blood-derived frozen B cell were purchased from AllCells (Alameda, CA, USA). Cell suspensions (final, 2 × 10 6 Save | Full Paper |
2022 | Pieles, O;Höring, M;Adel, S;Reichert, TE;Liebisch, G;Morsczeck, C; | Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle Cells | Stem cells international | 35903407 | Human dental follicle cells were purchased from AllCells (Alameda, CA, USA) and cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS) Save | Full Paper |
2022 | Schlichtner, S;Yasinska, IM;Ruggiero, S;Berger, SM;Aliu, N;Prunk, M;Kos, J;Meyer, NH;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV; | Expression of the Immune Checkpoint Protein VISTA Is Differentially Regulated by the TGF-β1 - Smad3 Signaling Pathway in Rapidly Proliferating Human Cells and T Lymphocytes | Frontiers in medicine | 35223897 | Medium was also supplemented with penicillin (50 IU/ml), and streptomycin sulfate (50 μg/ml). Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were also purchased from AllCells (Alameda, CA, USA) and handled according to | Full Paper |
2022 | Yoshimaru, K;Yamaza, T;Kajioka, S;Sonoda, S;Yanagi, Y;Matsuura, T;Yoshizumi, J;Oda, Y;Iwata, N;Takai, C;Nakayama, S;Taguchi, T; | Dental pulp stem cells as a therapy for congenital entero-neuropathy | Scientific reports | 35484137 | ies were passaged to expand the number of dDPSCs. Cells at P3 were used for the experiments. BMMSCs isolated from human whole bone marrow aspirates obtained from healthy adult volunteers (n = 3; AllCells, Alameda, CA, USA) and human skin fibroblasts | Full Paper |
2022 | Herrera, VLM;Walkey, AJ;Nguyen, MQ;Gromisch, CM;Mosaddhegi, JZ;Gromisch, MS;Jundi, B;Lukassen, S;Carstensen, S;Denis, R;Belkina, AC;Baron, RM;Pinilla-Vera, M;Mueller, M;Kimberly, WT;Goldstein, JN;Lehmann, I;Shih, AR;Eils, R;Levy, BD;Ruiz-Opazo, N; | A targetable 'rogue' neutrophil-subset, [CD11b+DEspR+] immunotype, is associated with severity and mortality in acute respiratory distress syndrome (ARDS) and COVID-19-ARDS | Scientific reports | 35379853 | ARDS (n = 4) for inertial microfluidic separation (BDL, RMB, MPV) according to respective institutional guidelines. Blood collection Fresh normal peripheral blood neutrophils were procured from AllCells, LLC (CA), which were shipped overnight in Isc | Full Paper |
2022 | Kim, DE;Shin, YH;Cho, JE;Myung, S;Kim, HG;Kim, KC;Park, CM;Yoon, CH; | Identification of 3-Oxindole Derivatives as Small Molecule HIV-1 Inhibitors Targeting Tat-Mediated Viral Transcription | Molecules (Basel, Switzerland) | 35956872 | d fetal bovine serum (all obtained from Gibco-BRL, Gaithersburg, MD, USA). The bl-DTR cells were additionally supplemented with 1 μg/mL puromycin and 200 μg/mL zeocin. The PBMCs were purchased from AllCells (Alameda, CA, USA) and cultured, as describ | Full Paper |
2022 | Saaid, A;Ensanya, A;Suzina, S;Nurul, A;Samsudin, A;Azlina, A; | Odontogenic induction of human amniotic membrane scaffold for dental pulp regeneration | Materials Chemistry and Physics | For this study, deciduous teeth stem cells (DTSCs), obtained from ALLCELLS, USA, were used at passage 4-8. The cells were allowed to grow in a-modified Eagle's medium (α-MEM) supplemented with 20% fetal bovine serum (FBS) and 1% penicillin/streptomyc | Full Paper | |
2022 | Jaimes, MC;Leipold, M;Kraker, G;Amir, EA;Maecker, H;Lannigan, J; | Full spectrum flow cytometry and mass cytometry: A 32-marker panel comparison | Cytometry. Part A : the journal of the International Society for Analytical Cytology | 35593221 | Frozen PBMCs from 5 donors were obtained from AllCells Alameda (Alameda, CA). Cells were provided aliquoted in 25 million cells per vial in freezing media and cryopreserved in liquid nitrogen until ready to use. Ethical review and regulatory complian | Full Paper |
2022 | Moore, KP;Schwaid, AG;Tudor, M;Park, S;Beshore, DC;Converso, A;Shipe, WD;Anand, R;Lan, P;Moningka, R;Rothman, DM;Sun, W;Chi, A;Cornella-Taracido, I;Adam, GC;Bahnck-Teets, C;Carroll, SS;Fay, JF;Goh, SL;Lusen, J;Quan, S;Rodriguez, S;Xu, M;Andrews, CL;Song, C;Filzen, T;Li, J;Hollenstein, K;Klein, DJ;Lammens, A;Lim, UM;Fang, Z;McHale, C;Li, Y;Lu, M;Diamond, TL;Howell, BJ;Zuck, P;Balibar, CJ; | A Phenotypic Screen Identifies Potent DPP9 Inhibitors Capable of Killing HIV-1 Infected Cells | ACS chemical biology | 36044633 | Cells were purchased from AllCells in cryopreserved vials. Cells were centrifuged at 500×g for 10 min at 4 C. The supernatant was removed, and cells were washed with 20 mL PBS. Save | Full Paper |
2022 | Zhang, H;Lv, X;Kong, Q;Tan, Y; | IL-6/IFN-γ double knockdown CAR-T cells reduce the release of multiple cytokines from PBMCs in vitro | Human vaccines & immunotherapeutics | 35049413 | rward TGGGAGCATGTGAATGCCATCC Reverse GTCGGCTCCTGGAGGTCAAACA β-actin Forward CGCCCCAGGCACCAGGGC Reverse GCTGGGGTGTTGAAGGT 2.3. IL-6/IFN-γ double KD CAR-T cell culture The PBMCs (Allcells, Cat: PB005F) were resuspended in CTSTM AIM | Full Paper |
2022 | Zhou, Z;Qin, M;Khodahemmati, S;Li, W;Niu, B;Li, J;Liu, Y;Gao, J; | Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis | International journal of environmental health research | 34102927 | We maintained HUVECs (Allcells, China) routinely in HUVEC complete culture medium (Allcells, China), supplemented with 10% fetal bovine serum (FBS), 2% growth factors, 100 U/mL Save | Full Paper |
2022 | Wang, X;Wong, LM;McElvain, ME;Martire, S;Lee, WH;Li, CZ;Fisher, FA;Maheshwari, RL;Wu, ML;Imun, MC;Murad, R;Toledo Warshaviak, D;Yin, J;Kamb, A;Xu, H; | A rational approach to assess off-target reactivity of a dual-signal integrator for T cell therapy | Toxicology and applied pharmacology | 35085592 | Informed-consent for use of primary T cells and donor collection protocols was approved by an Institutional Review Board (IRB) at Allcells or HemaCare. Allcells and HemaCare Save | Full Paper |
2022 | Andrews, SH;Klinker, MW;Bauer, SR;Marklein, RA; | Morphological landscapes from high content imaging reveal cytokine priming strategies that enhance mesenchymal stromal cell immunosuppression | Biotechnology and bioengineering | 34716713 | Human bone marrow-derived MSCs were obtained from four different donors purchased from Lonza, AllCells, and RoosterBio (see Table S1 for donor information). MSC culture conditions for the Lonza and AllCells (8F3560, 110877, PCBM1662) were chosen base | Full Paper |
2022 | Mandal, S;Sunagawa, SW;Prathipati, PK;Belshan, M;Shibata, A;Destache, CJ; | Targeted Immuno-Antiretroviral to Promote Dual Protection against HIV: A Proof-of-Concept Study | Nanomaterials (Basel, Switzerland) | 35683795 | were maintained in a complete DMEM medium (HiDMEM medium supplemented with 10% FBS and 1× AA) as standard protocol [14,15]. The human peripheral blood mononuclear cells (hPBMCs) were purchased from AllCells (Alameda, CA, USA) and maintained in comp | Full Paper |
2022 | Huang, P;Zhang, C;Delawary, M;Korchak, JA;Suda, K;Zubair, AC; | Development and evaluation of IL-6 overexpressing mesenchymal stem cells (MSCs) | Journal of tissue engineering and regenerative medicine | 34890489 | Human bone marrow derived mesenchymal stem cells (BMSCs) were isolated from commercial de-identified bone marrow (AllCells, Alameda, CA) from a healthy 28-year-old male Save | Full Paper |
2022 | Harper, T;Sharma, A;Kaliyaperumal, S;Fajardo, F;Hsu, K;Liu, L;Davies, R;Wei, YL;Zhan, J;Estrada, J;Kvesic, M;Nahrwold, L;Deisting, W;Panzer, M;Cooke, K;Lebrec, H;Nolan-Stevaux, O; | Characterization of an Anti-CD70 Half-Life Extended Bispecific T-Cell Engager (HLE-BiTE) and Associated On-Target Toxicity in Cynomolgus Monkeys | Toxicological sciences : an official journal of the Society of Toxicology | 35583313 | Human T cells (AllCells) or cynomolgus monkey or human peripheral blood mononuclear cells (PBMCs) (SNBL) were cocultured with target cells at an effector cell (E) to target cell (T) Save | Full Paper |
2022 | Wang, P;Zhang, J;Zhang, H;Zhang, F; | The role of MACF1 on acute myeloid leukemia cell proliferation is involved in Runx2-targeted PI3K/Akt signaling | Molecular and cellular biochemistry | 35857251 | Bone marrow CD34 + hematopoietic stem and progenitor cells (HSPCs) were purchased from AllCells (Alameda, CA, USA) and cultured in X-Vivo 15 media (Lonza, Basel, Switzerland) Save | Full Paper |
2022 | Shams, F;Bayat, H;Mohammadian, O;Mahboudi, S;Vahidnezhad, H;Soosanabadi, M;Rahimpour, A; | Advance trends in targeting homology-directed repair for accurate gene editing: An inclusive review of small molecules and modified CRISPR-Cas9 systems | BioImpacts : BI | 35975201 | A) Treatment group 1A (unedited HSCs from AllCells). The first mouse was found dead in Treatment group 2B, which contained edited bought HSCs (AllCells), had the highest bone Save | Full Paper |
2022 | Liu, Y;Ma, X;Yang, H;Li, X;Ma, Y;Ason, B;Liu, S;Hu, LA; | APLNR Regulates IFN-γ signaling via β-arrestin 1 mediated JAK-STAT1 pathway in melanoma cells | The Biochemical journal | 35084016 | Naive CD8+ T cells used for co-culture assay were obtained from Allcells and cultured in RPMI 1640 medium supplemented with 10% FBS, 1× HEPES, 1× PS, 2 mM Glutamine, 1 mM Save | Full Paper |
2022 | Venn-Watson, SK;Butterworth, CN; | Broader and safer clinically-relevant activities of pentadecanoic acid compared to omega-3: Evaluation of an emerging essential fatty acid across twelve primary human cell-based disease systems | PloS one | 35617322 | lowed ethical guidelines and documented informed consent: Cell Applications, Inc., CellzDirect, Celsis-IVT, Leukolab, Life Technologies, Lonza, ScienCell, Hemacare Corporation, Stemcell Technologies, AllCells, Physician’s Plasma Alliance, Lifeline Ce | Full Paper |
2022 | Okada, Y;Kawashima, N;Noda, S;Murano, H;Han, P;Hashimoto, K;Kaneko, T;Okiji, T; | VEGFA promotes odonto/osteoblastic differentiation in dental pulp stem cells via ERK/p38 signaling | Journal of Dental Sciences | SHEDs (P5, DP001F; AllCells, Alameda, CA, USA) were cultured in alpha-modified Eagle's minimum essential medium (α-MEM) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and an antibiotic and antifungal soluti | Full Paper | |
2022 | Chen, W;Qie, C;Hu, X;Wang, L;Jiang, J;Liu, W;Liu, J; | A small molecule inhibitor of VSIG-8 prevents its binding to VISTA | Investigational new drugs | 35404016 | PBMCs were purchased from Allcells Biotechnology (Shanghai) Co., LTD, and we got a statement confirming that informed consent was obtained from all subjects, the attachment is on response. Jurkat cells were cultured in RPMI-1640 medium (Biological In | Full Paper |
2022 | Han, Y;Bai, X;Wang, X; | Exosomal myeloperoxidase as a biomarker of deep venous thrombosis | Annals of translational medicine | 35242854 | al microscope (Olympus). HUVECs culture We purchased HUVECs from American Type Culture Collection and cultured them in endothelial cell complete medium containing endothelial cell growth supplement (AllCells, Alameda, CA, USA) at 37 °C in a humidifi | Full Paper |
2022 | Sato, M;Fujii, K;Takagi, H;Shibuya, I;Oka, D;Yamaya, N;Hagita, H;Matsumoto, M;Inagaki, K; | Antibacterial and Immunosuppressive Effects of OPS-2071, a Candidate Therapy for Inflammatory Bowel Disease | Digestive diseases and sciences | 34463880 | Mouse splenocytes were prepared from eight-week-old female BALB/c mice, and human peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Alameda, CA). To Save | Full Paper |
2022 | Skaddan, MB;Wooten, DW;Wilcox, KC;Voorbach, MJ;Reuter, DR;Jia, ZJ;Foster-Duke, KD;Hickson, JA;Vaidyanathan, S;Reed, AD;Tovcimak, AE;Guo, Q;Comley, RA;Lee, L;Finnema, SJ;Mudd, SR; | [18F]BTK-1: A Novel Positron Emission Tomography Tracer for Imaging Bruton's Tyrosine Kinase | Molecular imaging and biology | 35482146 | Purpose Bruton's tyrosine kinase (BTK) is a key component of B cell receptor (BCR) signaling, and as such a critical regulator of cell proliferation and survival. Aberrant BCR signaling is Save | Full Paper |
2022 | Wu, C;Wang, X;Shang, H;Wei, H; | Construction of a Humanized PBMC-PDX Model to Study the Efficacy of a Bacterial Marker in Lung Cancer Immunotherapy | Disease markers | 36072895 | , Ltd., and the RPMI-1640 Complete Medium (10%FBS; Gibco) was used for culture. When the cells reached 80% confluence, they were passed on. 2.3. PBMCs Immune Reconstitution PBMCs were purchased from AllCells and processed according to the company's | Full Paper |
2022 | Ryan, DE;Diamant-Levi, T;Steinfeld, I;Taussig, D;Visal-Shah, S;Thakker, S;Lunstad, BD;Kaiser, RJ;McCaffrey, R;Ortiz, M;Townsend, J;Welch, WRW;Singh, M;Curry, B;Dellinger, DJ;Bruhn, L; | Phosphonoacetate Modifications Enhance the Stability and Editing Yields of Guide RNAs for Cas9 Editors | Biochemistry | 35436085 | Human primary T cells (LP, CR, CD3+, NS) were obtained from AllCells (Alameda, CA) and were cultured in RPMI 1640 plus GlutaMax media supplemented with 10% fetal bovine serum, 5 ng/mL human IL-7, and 5 ng/mL human IL-15 (Gibco). Primary T cells were | Full Paper |
2022 | Darbaniyan, F;Zheng, H;Kanagal-Shamanna, R;Lockyer, P;Montalban-Bravo, G;Estecio, M;Lu, Y;Soltysiak, KA;Chien, KS;Yang, H;Sasaki, K;Class, C;Ganan-Gomez, I;Do, KA;Garcia-Manero, G;Wei, Y; | Transcriptomic Signatures of Hypomethylating Agent Failure in Myelodysplastic Syndromes and Chronic Myelomonocytic Leukemia | Experimental hematology | 36150563 | CD34 + cells isolated from healthy individuals (AllCells, Alameda CA) and from patients with MDS or CMML were plated into human methylcellulose colony formation medium Methocult- Save | Full Paper |
2022 | Valdez, BC;Yuan, B;Murray, D;Nieto, Y;Popat, U;Andersson, BS; | Enhanced cytotoxicity of bisantrene when combined with venetoclax, panobinostat, decitabine and olaparib in acute myeloid leukemia cells | Leukemia & lymphoma | 35188042 | Bisantrene (Bis), a topoisomerase-II inhibitor, is less cardiotoxic than the current anthracyclines. Its synergistic cytotoxicity with newly developed antineoplastic drugs has not been Save | Full Paper |
2022 | Fan, F;Liu, F;Shen, P;Tao, L;Zhang, H;Wu, H; | Salvianolic acid B, a new type I IRE1 kinase inhibitor, abrogates AngII-induced angiogenesis by interacting with IRE1 in its active conformation | Clinical and experimental pharmacology & physiology | 36153795 | Primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China) and cultured in EBM media (AllCells ) according to the manufacturer's Save | Full Paper |
2022 | Tong, Q;Liu, H;Qi, Q;Dai, C;Yang, T;Qian, F; | Development of a fully human anti-GITR antibody with potent antitumor activity using H2L2 mice | FEBS open bio | 35674216 | h well, and luminescence values were measured using a Varioskan LUX (Thermo, Carlsbad, CA, USA). T‐cell activation assays Total T cells from frozen human peripheral blood mononuclear cells (PBMCs, AllCells, Alameda, CA, USA) were first isolated usin | Full Paper |
2022 | Yang, M;Tkach, D;Boyne, A;Kazancioglu, S;Duclert, A;Poirot, L;Duchateau, P;Juillerat, A; | Optimized two-step electroporation process to achieve efficient nonviral-mediated gene insertion into primary T cells | FEBS open bio | 34510816 | ineering in human primary cells and provide a foundation for further advances using nonviral gene editing methods. Materials and methods T‐cell culture Cryopreserved human PBMCs were acquired from ALLCELLS. PBMCs were cultured in X‐vivo‐15 media (Lo | Full Paper |
2022 | Pieles, O;Reichert, TE;Morsczeck, C; | Protein kinase A is activated during bone morphogenetic protein 2-induced osteogenic differentiation of dental follicle stem cells via endogenous parathyroid hormone-related protein | Archives of oral biology | 35338829 | Human dental follicle stem cells (Catalog number FT002F, Lot number ACF001) were commercially obtained from AllCells (Emeryville, USA) and cultured in Dulbecco's Modified Eagle's Medium (DMEM) high glucose (Sigma Aldrich, St. Louis, USA) with 10% fet | Full Paper |
2022 | Qian, J;Yu, J;Zhu, X;Liang, S; | MiR-335 promotes corneal neovascularization by Targeting EGFR | BMC ophthalmology | 35701740 | surgery and on day 3, 7, 11 and 14. The Animal Research Committee of the Affiliated Hospital of Nantong University approved all animal studies. Cell culture and different treatments HUVECs were from AllCells (Shanghai, China). They were put in an RP | Full Paper |
2022 | Wang, W;Tang, W;Shan, E;Zhang, L;Chen, S;Yu, C;Gao, Y; | MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FAS | European journal of histochemistry : EJH | 35638591 | cells (HUVEC) in vitro. Materials and Methods Cell culture HUVEC cells have played a major role as a model system for the study of the regulation of endothelial cell function. HUVEC cells (Shanghai AllCells Biotech Co., Ltd. Shanghai, China) were cu | Full Paper |
2022 | Koh, H;Zhen, X;Kim, J;Ha, HY;Lee, JH; | Generation and characterization of human umbilical cord blood-derived induced pluripotent stem cells (KRIBBi005-A) | Stem cell research | 35085946 | Ethical approval Approval obtained by Public Institutional Bioethics Committee designated by the MOHW (P01-201909-31-002). Cord blood mononuclear cells were purchased (AllCells LLC) | Full Paper |
2022 | Hashim, SNM;Azlina, A; | Ultrastructural morphological changes of VEGF treated-stem cells from human exfoliated deciduous teeth cultured on human amniotic membrane | Acta Microscopica | SHED (AllCells, USA) were cultured in a complete medium made of the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, USA), supplemented with 15% of fetal bovine serum (FBS) (Gibco, USA) and 50 U/ml of penicillin-st | Full Paper | |
2022 | Bai, Y;Zhang, X;Zheng, J;Liu, Z;Yang, Z;Zhang, X; | Overcoming high level adenosine-mediated immunosuppression by DZD2269, a potent and selective A2aR antagonist | Journal of experimental & clinical cancer research : CR | 36229853 | bR, CHO-A1R and CHO-A3R) were purchased from GenScript. Cell lines RM-1, B16F10 and Pan02 were obtained from the American Type Culture Collection (ATCC). Cryopreserved human PBMCs were purchased from AllCells. All CHO cells were cultured in Ham’s F12 | Full Paper |
2022 | Kartha, VK;Duarte, FM;Hu, Y;Ma, S;Chew, JG;Lareau, CA;Earl, A;Burkett, ZD;Kohlway, AS;Lebofsky, R;Buenrostro, JD; | Functional inference of gene regulation using single-cell multi-omics | Cell genomics | 36204155 | AILS Human peripheral blood mononuclear cells Cryopreserved human peripheral blood mononuclear cells (PBMCs) and isolated peripheral blood CD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from AllCells (see Table S1 for catalog numbers and don | Full Paper |
2022 | Riaz, S;Azlina, A;Mahmood, Z;Htun, AT; | Long-term treatment of dentine with triple antibiotic paste promotes stem cell viability and attachment | Journal of Taibah University Medical Sciences | 35983454 | , the dentine chips were removed from the well plates and air-dried on a filter paper. The samples were then stored in a sterile container at 4 °C until further use. Cell culture Commercial DPSCs (AllCells, USA) were used from passage 7 to 9 at 70%- | Full Paper |
2022 | Salam, N;Gibson, IR; | Lithium ion doped carbonated hydroxyapatite compositions: Synthesis, physicochemical characterisation and effect on osteogenic response in vitro | Biomaterials advances | 35939955 | the American Type Culture Collection (via LGC standards, UK), PromoCell (Germany), and AllCells (USA), respectively. Cells were maintained in routine culture conditions using: high glucose DMEM with 10 % FBS and 4 mM l-glutamine for C2C12 cells, Gibc | Full Paper |
2022 | Nakauchi, Y;Azizi, A;Thomas, D;Corces, MR;Reinisch, A;Sharma, R;Cruz Hernandez, D;Köhnke, T;Karigane, D;Fan, A;Martinez-Krams, D;Stafford, M;Kaur, S;Dutta, R;Phan, P;Ediriwickrema, A;McCarthy, E;Ning, Y;Phillips, T;Ellison, CK;Guler, GD;Bergamaschi, A;Ku, CJ;Levy, S;Majeti, R; | The Cell Type-Specific 5hmC Landscape and Dynamics of Healthy Human Hematopoiesis and TET2-Mutant Preleukemia | Blood cancer discovery | 35532363 | Normal donor human BM cells were obtained fresh from AllCells, and peripheral blood cells Human BM samples (CD34+ cell enriched) were purchased from AllCells. Mononuclear Save | Full Paper |
2022 | Baskar, R;Chen, AF;Favaro, P;Reynolds, W;Mueller, F;Borges, L;Jiang, S;Park, HS;Kool, ET;Greenleaf, WJ;Bendall, SC; | Integrating transcription-factor abundance with chromatin accessibility in human erythroid lineage commitment | Cell reports methods | 35463156 | # ab181544 Cleaved caspase 3-PE BD Biosciences Cat# 550821, RRID:AB_393906 ------------------------- Biological samples ------------------------- Adult bone marrow All Cells Inc https://allcells.com/research-grade-tissue-products/bone-m | Full Paper |
2022 | Kwak, J;Choi, W;Bae, Y;Kim, M;Choi, S;Oh, W;Jin, H; | HLA-A2 Promotes the Therapeutic Effect of Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Hyperoxic Lung Injury | Bioengineering (Basel, Switzerland) | 35447737 | St. Louis, MO, USA) for 1 h at 37 °C. The UCB-MSCs (1 × 103 cells/well) were seeded and maintained at 37 °C in a humidified incubator for 4 h and then co-cultured with PBMCs (1 × 105 cells/well; Allcells, Boston, MA, USA) from different donors. Phyto | Full Paper |
2022 | Vivanco Gonzalez, N;Oliveria, JP;Tebaykin, D;Ivison, GT;Mukai, K;Tsai, MM;Borges, L;Nadeau, KC;Galli, SJ;Tsai, AG;Bendall, SC; | An optimized protocol for phenotyping human granulocytes by mass cytometry | STAR protocols | 35434655 | y donors (with unknown allergy status) were obtained from the Stanford Blood Center (Palo Alto, CA, USA). Healthy bone marrow and peripheral blood samples paired from the same donor were ordered from AllCells (Alameda, CA, USA). Samples from patients | Full Paper |
2022 | Bell-Glenn, S;Thompson, JA;Salas, LA;Koestler, DC; | A Novel Framework for the Identification of Reference DNA Methylation Libraries for Reference-Based Deconvolution of Cellular Mixtures | Frontiers in bioinformatics | 35419567 | Cell Mixture Reconstruction Experiment Purified cells taken from normal human subjects were purchased from AllCells LLC (Emeryville, CA). Namely, granulocytes, monocytes, CD4T, Save | Full Paper |
2022 | Shui, S;Wang, S;Liu, J; | Systematic Investigation of the Effects of Multiple SV40 Nuclear Localization Signal Fusion on the Genome Editing Activity of Purified SpCas9 | Bioengineering (Basel, Switzerland) | 35200436 | s medium (DMEM) supplemented with 10% (v/v) FBS, 100 U/mL penicillin and 100 U/mL streptomycin. The medium of EGFP reporter contained an additional 50 U/mL hygromycin. Human dermal fibroblasts (hDF, AllCells) were acquired from Cell Applications (Lot | Full Paper |
2022 | Li, L;Xu, H;Gao, L; | Doxorubicin-Loaded Black Phosphorus Multifunctional Nanodelivery System Combined with Photothermal Therapy Promotes Immunogenic Death of Prostate Cancer PC-3 Cells | SSRN Electronic Journal | Beijing, China). Dendritic cells (DCs) were 197obtained from AllCells (San Francisco, USA). FITC anti198human CD11c Antibody, Alexa Fluor® 647 anti-human 199CD80 Antibody, PE anti-human CD86 Antibody, and 200PerCP/Cyanine5.5 anti-human HLA-DR Antibod | Full Paper | |
2022 | Calderon, A;Kim, Y;Favaro, P;Baskar, R;Valainis, J;Reynolds, W;Borges, L;Glass, D;Tsai, A;Bendall, S; | Multi-Omic Single Cell Identification of Lin- CD34- Natural Killer Cell Progenitors in Human Hematopoiesis | SSRN Electronic Journal | Deidentified human blood (n=5) and BM (n=7) were obtained from healthy adult donors (Stanford Blood Center, Allcells). All samples were obtained under informed consent and in Save | Full Paper | |
2022 | Lee, J;Jonus, H;Sadanand, A;Branella, G;Maximov, V;Suttapitugsakul, S;Schniederjan, M;Shim, J;Parwani, K;Fedanov, A;Pilgrim, A;Silva, J;Doering, C;Wu, R;Spencer, H;Goldsmith, K; | Identification and Targeting of Protein Tyrosine Kinase 7 (PTK7) as an Immunotherapy Candidate for Neuroblastoma Cellular Therapy | SSRN Electronic Journal | GD2-targeted immunotherapy improves survival for pediatric patients with neuroblastoma (NB). However, toxicities associated with on-target, off-tumor activity occur. Furthermore, GD2 Save | Full Paper | |
2022 | O'Brien, G;Cecotka, A;Manola, K;Pagoni, M;Polańska, J;Badie, C; | Epigenetic Signature of Ionising Radiation In Therapy-Related AML Patients | SSRN Electronic Journal | Bone marrow aspirate samples were also obtained from 5 healthy normal donors (4 males, 1 female, age 21-33) from AllCells® (Alamada, USA). The project was in accordance with the declaration of Helsinki. Informed consent was provided from patients and | Full Paper | |
2022 | Qian, T;Qi, B;Fei, Y;Li, J;Luo, L;Song, Y;Sheng, S;Lv, B;Huang, X;Wang, X; | Deletion of PLD2 Alleviates LPS Induced Acute Lung Injury by Inhibiting STAT3 Phosphorylation and Regulating Endothelial Tight Junctions | Research Square | HUVECs were purchased from the Shanghai cell bank and cultured in a 5% CO2 incubator at 37oC in a complete culture medium (AllCells, Shanghai, China). Cells were cultured to 85- Save | Full Paper | |
2022 | Li, W;Li, J;Hu, X;Xu, L;Liu, X;Qian, Z;Jin, L;Zhang, Y;Wei, J;Liu, X; | Tumor suppression by Chinese cordyceps extract via anti-angiogenesis | Research Square | Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in special medium for HUVEC cells at 37C with 5% CO2. CCE was Save | Full Paper | |
2022 | Li, L;Chen, X;Li, Y;Lou, L; | Pharmacological characterization of GR1803, a novel BCMA × CD3 bispecific antibody for multiple myeloma treatment | Research Square | The MM cell lines NCI-H929, MM.1S, RPMI-8226, and U266 were obtained from the American Type Culture Collection (Manassas, VA, USA). Fresh peripheral blood mononuclear cells (PBMCs) were obtained from AllCells Co. (Shanghai, China). Natural killer (NK | Full Paper | |
2022 | Yan, F;Luo, B;Chen, C;Tan, B;Cui, D;Wang, M;Qian, J; | Inhibiting PHD2 in bone marrow mesenchymal stem cells in an inflammatory microenvironment facilitates periodontal repair in SD rats | Research Square | Primary BMMSCs of SD rats were isolated and puri ed by AllCells (Alameda, CA, USA), and their phenotype was identi ed. The cells were recovered and cultured in low glucose Save | Full Paper | |
2022 | Guedes, F;Branquinho, M;Biscaia, S;Alvites, R;Sousa, A;Lopes, B;Sousa, P;Rêma, A;Amorim, I;Faria, F;Patrício, T;Bugalho, A;Alves, N;Maurício, A; | Gamma Irradiation Processing on 3D PCL Devices - A Preliminary Biocompatibility Assessment | preprints | Human Dental Pulp stem/stromal cells (hDPSCs) obtained from AllCells, LLC (Cat. DP0037F, Lot Nº DPSC090411-01) were maintained in MEM α, GlutaMAX™ Supplement, no nucleosides (Gibco, 32561029), supplemented with 10% (v/v) fetal bovine serum (FBS) (Gib | Full Paper | |
2022 | Tokatlian, T;Asuelime, G;Naradikian, M;Mock, J;Daris, M;Martin, A;Toledo Warshaviak, D;Kamb, A;Hamburger, A; | Chimeric Antigen Receptors Directed at Mutant KRAS Exhibit an Inverse Relationship Between Functional Potency and Neoantigen Selectivity | Cancer Research Communications | Peripheral blood mononuclear cells (PBMC) from healthy donors were obtained from AllCells. PBMC collection and donor written informed consent were approved by an Institutional Save | Full Paper | |
2022 | Boccacci, Y;Dumont, N;Doyon, Y;Laganière, J; | Accessory-cell-free differentiation of hematopoietic stem and progenitor cells into mature red blood cells | bioRxiv | The culture and ex vivo engineering of red blood cells (RBCs) can help characterize genetic variants, model diseases, and may eventually spur the development of applications in Save | Full Paper | |
2022 | Checkley, M;Luttge, B;Dobrowolski, C;Wald, D;McMahon, D;Haidar, G;Sobolewski, M;Enick, P;Cyktor, J;Mellors, J;Karn, J; | Reduction of the HIV-1 reservoir in T cells from persons with HIV-1 on suppressive antiretroviral therapy using expanded natural killer cells ex vivo | bioRxiv | For this study leukapheresis packs from 5 HIV-1-negative donors were purchased from AllCells. 437 For HIV+ participants, PBMCs from well-suppressed ART-treated patients from the Save | Full Paper | |
2022 | Shabaneh, T;Moffett, H;Stull, S;Derezes, T;Tait, L;Park, S;Riddell, S;Lajoie, M; | Safety switch optimization enhances antibody-mediated elimination of CAR T cells | bioRxiv | Natural killer cells were isolated from cryopreserved, T cell depleted (CD4-/CD8-) PBMC (AllCells) by negative selection using the EasySep Human NK Cell Kit (Stemcell) according to the manufacturer’s protocol. To activate their cytolytic function, is | Full Paper | |
2022 | Wisdom, K;Suijker, J;van den Broek, L;Sridharan, B;Grandhi, T;Cheng, A;Lamb, M;Titus, S;Gehman, A;Poore, D;Shah, N;Cheng, S;Kim, E;Griffin, S;Ekert, J; | Lung Tumor Microphysiological System with 3D Endothelium to Evaluate Modulators of T-Cell Infiltration | bioRxiv | AllCells and shipped to the MIMETAS research facility, or they 653 were isolated from AllCells For this, T-cells were isolated from full 654 fresh leukopaks (AllCells). Leukopaks were Save | Full Paper | |
2022 | Dogan, M;Karhan, E;Kozhaya, L;Placek, L;Chen, X;Yigit, M;Unutmaz, D; | Engineering human Mucosal Associated Invariant T (MAIT) cells with chimeric antigen receptors for cancer immunotherapy1 | bioRxiv | Healthy adult blood was obtained from AllCells Quincy, MA. PBMCs were isolated using Ficoll- 100 paque plus (GE Health care). CD4+ T, CD8+ T, CD19+ B cells, CD14+ monocytes Save | Full Paper | |
2022 | Swift, M;Horns, F;Quake, S; | Fate Bias and Transcriptional Memory of human B cells | bioRxiv | from a healthy male aged 50-55 were obtained from AllCells. Mononuclear cells were isolated using Ficoll gradient and Red Blood Cell Lysis. Plasma cells from the bone marrow were isolated using StemCell EasySepEasySep™ Human CD138 Positive Selection | Full Paper | |
2022 | White, B;de Reyniès, A;Newman, A;Waterfall, J;Lamb, A;Petitprez, F;Valdeolivas, A;Lin, Y;Li, H;Xiao, X;Wang, S;Zheng, F;Yang, W;Yu, R;Guerrero-Gimenez, M;Catania, C;Lang, B;Domanskyi, S;Bertus, T;Piermarocchi, C;Monaco, G;Caruso, F;Ceccarelli, M;Yu, T;Guo, X;Coller, J;Maecker, H;Duault, C;Shokoohi, V;Patel, S;Liliental, J;Simon, S;Saez-Rodriguez, J;Heiser, L;Guinney, J;Gentles, A;Tumor Deconvolution DREAM Challenge consortium, ; | Community assessment of methods to deconvolve cellular composition from bulk gene expression | bioRxiv | Immune and stromal cells provided by StemExpress and AllCells were isolated according to ) and the rest assigned immune cells from AllCells wherever availability allowed (Tables S5 Save Cached | Full Paper | |
2022 | Simmons, S;Lithwick-Yanai, G;Adiconis, X;Oberstrass, F;Iremadze, N;Geiger-Schuller, K;Thakore, P;Frangieh, C;Barad, O;Almogy, G;Rozenblatt-Rosen, O;Regev, A;Lipson, D;Levin, J; | Single cell RNA-seq by mostly-natural sequencing by synthesis | bioRxiv | We purchased 9 cryopreserved human PMBC samples (AllCells). We thawed PBMC vials in a 37C water bath for ∼2 minutes. A quick counting revealed high viability (>90%) in all Save | Full Paper | |
2022 | Seenappa, L;Jakubowski, A;Steinbuck, M;Palmer, E;Haqq, C;Carter, C;Fontenot, J;Villinger, F;McNeil, L;DeMuth, P; | Programming the lymph node immune response with Amphiphile-CpG induces potent cellular and humoral immunity following COVID-19 subunit vaccination in mice and non-human primates | bioRxiv | who had recovered from SARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and ALLCELLS (Alameda, CA). All samples were received and stored frozen at −80°C until analysis | Full Paper | |
2022 | Lutter, L;ter Linde, J;Brand, E;van Konijnenburg, D;Roosenboom, B;Talabur-Horje, C;Oldenburg, B;van Wijk, F; | Compartment-driven imprinting of intestinal CD4 (regulatory) T cells in inflammatory bowel disease and homeostasis | bioRxiv | Cluster 1 (18.6% of allcells) was characterized by KLF2, SELL, CCR7, TCF7 and LEF1, and thus resembled recently migrated/recirculating T cells (figure 4A). This cluster contained both intraepithelial and lamina propria T cells (figure 4E). Similarly, | Full Paper | |
2022 | Jaffe, D;Shahi, P;Adams, B;Chrisman, A;Finnegan, P;Raman, N;Royall, A;Tsai, F;Vollbrecht, T;Reyes, D;McDonnell, W; | Functional antibodies exhibit light chain coherence | bioRxiv | We titrated and developed the B cell fractionation panel using 20 million fresh PBMCs (AllCells, catalog # 3050363) from a healthy human donor whose cells were not used to generate Save | Full Paper | |
2022 | Persad, S;Choo, Z;Dien, C;Masilionis, I;Chaligné, R;Nawy, T;Brown, C;Pe’er, I;Setty, M;Pe’er, D; | SEACells: Inference of transcriptional and epigenomic cellular states from single-cell genomics data | bioRxiv | Cryopreserved bone marrow stem/progenitor CD34+ cells from a healthy donor were purchased from AllCells, LLC. (catalog no. ABM022F) and stored in vapor phase nitrogen. Vial was removed from the storage and immediately thawed at 37 °C in a water bath | Full Paper | |
2022 | Stuart, T;Hao, S;Zhang, B;Mekerishvili, L;Landau, D;Maniatis, S;Satija, R;Raimondi, I; | Nanobody-tethered transposition allows for multifactorial chromatin profiling at single-cell resolution | bioRxiv | Cryopreserved healthy donor PBMCs were isolated from mobilized peripheral blood, BMMCs were purchased from AllCells. After thawing into DMEM with 10% FBS, the cells were spun Save | Full Paper | |
2022 | MacMullan, M;Dunn, Z;Qu, Y;Wang, P;Graham, N; | Phospho-proteomic analysis of CAR-T cell signaling following activation by antigen-presenting cancer cells | bioRxiv | cDNAs from BM CD34+, PB CD34+, BM MNCs, PB MNCs and BM MSCs purchased from AllCells (Emeryville, CA, USA) was obtained from a population of positive cells (purity exceeding 95%) collected from five individual donors. The level of cognate cDNA was mea | Full Paper | |
2022 | Dogan, M;Kozhaya, L;Placek, L;Karabacak, F;Yigit, M;Unutmaz, D; | Targeting SARS-CoV-2 infection through CAR-T like bispecific T cell engagers incorporating ACE2 | bioRxiv | Healthy adult blood was obtained from AllCells. PBMCs were isolated using Ficoll-paque plus (GE Health care). CD8 T cells were purified using Dynal CD8 Positive Isolation Kit (from Save | Full Paper | |
2022 | Yashar, W;Curtiss, B;Coleman, D;Van-Campen, J;Kong, G;Macaraeg, J;Estabrook, J;Demir, E;Long, N;Bottomly, D;McWeeney, S;Tyner, J;Druker, B;Maxson, J;Braun, T; | Disruption of the MYC Super-Enhancer Complex by Dual Targeting of FLT3 and LSD1 in Acute Myeloid Leukemia | bioRxiv | Whole bone marrow was obtained (AllCells) and CD34+ cells were selected using CD34 MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions. For the colony assay, 500 CD34+ cells were used per replicate and plated in MethoCult™ H4435 | Full Paper | |
2022 | Aksöz, M;Gafencu, G;Stoilova, B;Buono, M;Meng, Y;Jakobsen, N;Metzner, M;Clark, S;Beveridge, R;Thongjuea, S;Vyas, P;Nerlov, C; | Identification and Age-dependent Increase of Platelet Biased Human Hematopoietic Stem Cells | bioRxiv | Fresh BM from healthy young volunteers (21 to 34 years old males) were purchased from AllCells (Berkeley, CA) or Lonza (Lonza Bioscience, Basel, Switzerland). Bone marrow from older donors was obtained from orthoplastic surgery after | Full Paper | |
2022 | Xiang Ping, M;Zhi, H;Aziz, N;Hadri, N;Ghazalli, N;Yusop, N; | Optimization of agarose-alginate hydrogel bead components for encapsulation and transportation of stem cells | Journal of Taibah University Medical Sciences | Stem cells from human exfoliated deciduous teeth (SHED) purchased from AllCells USA were revived from the cryovial and maintained in culture before encapsulation. SHED from Save | Full Paper | |
2022 | Marcisak, EF; | Leveraging single-cell genomics to uncover clinical and preclinical responses to cancer immunotherapy | Thesis | GFP due to transduction with pBMN-IRES-EGFP containing the FcγRIIIA construct. All NK cell lines were cultured as previously described[231]. Fresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selected | Full Paper | |
2022 | Nesterenko, PA; | Characterizing the T cell immune response through the receptor-ligand interaction | Thesis | Cell culture: Cryo preserved peripheral blood mononuclear cells (PBMCs) were commercially purchased (Allcells and Hemacare). PBMCs were thawed in a water bath set to 37C, transferred to 50 mL conical tube, 1 mL of warm R10 media was added drop wise a | Full Paper | |
2022 | Chu, J; | Ligament tissue engineering: assessing differentiation potential of dental follicle cells in a self-assembly three-dimensional organoid model. | Thesis | according to AllCells company regulations, further donor information such gender and exact age was not provided to the purchaser). The PDL-hTERT immortal cell line has already Save | Full Paper | |
2022 | Plasek, LM; | Self-Silencing: An HIV Induced Mechanism for T Cell Quiescence and HIV Latency | Thesis | Peripheral blood mononuclear cells from HIV negative donors were purchased from AllCells. Naive CD4+ T cells were isolated using the EasySepTM Human Naive CD4+ T Cell Save | Full Paper | |
2022 | BOSYKH, D; | Mechanisms and Biological Consequences of Mutual Reprogramming by Melanoma Cells and Normal Dermal Fibroblasts | Thesis | Cell lines and medium Primary human neonatal dermal fibroblasts (NDFs; AllCells, LLC) were combined from three individual donors. NDFs were routinely tested for mycoplasma and confirmed to be negative using the MycoAlert mycoplasma detection kit (Lon | Full Paper | |
2022 | Oostindie, SC;Rinaldi, DA;Zom, GG;Wester, MJ;Paulet, D;Al-Tamimi, K;van der Meijden, E;Scheick, JR;Wilpshaar, T;de Jong, B;Hoff-van den Broek, M;Grattan, RM;Oosterhoff, JJ;Vignau, J;Verploegen, S;Boross, P;Beurskens, FJ;Lidke, DS;Schuurman, J;de Jong, RN; | Logic-gated antibody pairs that selectively act on cells co-expressing two antigens | Nature biotechnology | 35879362 | PBMCs derived from patients with CLL were commercially obtained from Discovery Life Sciences. Characteristics of patients with CLL are summarized in Supplementary Save | Full Paper |
2022 | Schuler, M;Cuppens, K;Ploenes, T;Vanbockrijck, M;Wiesweg, M;Darwiche, K;Schramm, A;Maes, B;Hegedus, B;Schildhaus, H;Hautzel, H;Theegarten, D;Baas, P;Hartemink, K;Du Pont, B;Aigner, C; | LBA37 A randomized, multicentric phase II study of preoperative nivolumab plus relatlimab or nivolumab in patients with resectable non-small cell lung cancer (NEOpredict-Lung) | Annals of Oncology | cology, AstraZeneca, Eisai, Takeda, ZytoVision, Zytomed Systems, Molecular Health; Financial Interests, Institutional, Research Grant: Novartis Oncology; Financial Interests, Personal, Full or part-time Employment: Targos Molecular Pathology Inc.. H. | Full Paper | |
2022 | Baraibar Argota, I;Garcia Rodriguez, A;Salvà Ballabrera, F;Ros Montana, F;Saoudi Gonzalez, N;Comas, R;Castillo, G;Sanchis, M;Hernando Cubero, J;García-Alvarez, A;Capdevila Castillon, J;Martí, M;Landolfi, S;Espin, E;Nuciforo, P;Vivancos, A;Tabernero, J;Elez Fernandez, M; | 332P Impact of the COVID-19 pandemic in the early-onset colorectal cancer (EOCRC) | Annals of Oncology | ersonal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. A. Vivancos: Financial Interests, Personal, Advisory Board: Roc | Full Paper | |
2022 | Lau, D;Khare, S;Stein, MM;Jain, P;Gao, Y;BenTaieb, A;Rand, TA;Salahudeen, AA;Khan, AA; | Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancer | Nature communications | 35831288 | Samples for single-cell multi-omic sequencing were previously frozen dissociated tumor cells (DTCs) (Discovery Life Sciences, Huntsville, AL). DTCs were thawed and Save;xpression analyses were performed on log10-transformed counts. Single-cell mul | Full Paper |
2022 | Leung, DH;Devaraj, S;Goodrich, NP;Chen, X;Rajapakshe, D;Ye, W;Andreev, V;Minard, CG;Guffey, D;Molleston, JP;Bass, LM;Karpen, SJ;Kamath, BM;Wang, KS;Sundaram, SS;Rosenthal, P;McKiernan, P;Loomes, KM;Jensen, MK;Horslen, S;Bezerra, JA;Magee, JC;Merion, RM;Sokol, RJ;Shneider, BL;For ChiLDReN, ; | Serum Biomarkers Correlated with Liver Stiffness Assessed in a Multi-center Study of Pediatric Cholestatic Liver Disease | Hepatology (Baltimore, Md.) | 36069569 | similar distributions of age- and sex-matched children with normal liver biochemistries and without known liver disease served as case controls (Discovery Life Sciences, Save | Full Paper |
2022 | Yap, TA;Gainor, JF;Callahan, MK;Falchook, GS;Pachynski, RK;LoRusso, P;Kummar, S;Gibney, GT;Burris, HA;Tykodi, SS;Rahma, OE;Seiwert, TY;Papadopoulos, KP;Blum Murphy, M;Park, H;Hanson, A;Hashambhoy-Ramsay, Y;McGrath, L;Hooper, E;Xiao, X;Cohen, H;Fan, M;Felitsky, D;Hart, C;McComb, R;Brown, K;Sepahi, A;Jimenez, J;Zhang, W;Baeck, J;Laken, H;Murray, R;Trehu, E;Harvey, CJ; | First-in-Human Phase I/II ICONIC Trial of the ICOS Agonist Vopratelimab Alone and with Nivolumab: ICOS-High CD4 T-Cell Populations and Predictors of Response | Clinical cancer research : an official journal of the American Association for Cancer Research | 35511938 | Full Paper | |
2022 | Shalev, TJ;Gamal El-Dien, O;Yuen, MMS;Shengqiang, S;Jackman, SD;Warren, RL;Coombe, L;van der Merwe, L;Stewart, A;Boston, LB;Plott, C;Jenkins, J;He, G;Yan, J;Yan, M;Guo, J;Breinholt, JW;Neves, LG;Grimwood, J;Rieseberg, LH;Schmutz, J;Birol, I;Kirst, M;Yanchuk, AD;Ritland, C;Russell, JH;Bohlmann, J; | The western redcedar genome reveals low genetic diversity in a self-compatible conifer | Genome research | 36109148 | University of British Columbia; tal.shalev@msl.ubc.ca.++University of British Columbia.++University of British Columbia.++Department of Energy Joint Genome Institute.++Canada's Michael Smith Genome Sciences Centre.++Canada's Michael Smith Genome Scie | Full Paper |
2022 | Cramer, DAT;Franc, V;Caval, T;Heck, AJR; | Charting the Proteoform Landscape of Serum Proteins in Individual Donors by High-Resolution Native Mass Spectrometry | Analytical chemistry | 36074704 | ditions. Experimental Procedures Individual serum samples from six healthy donors were provided by Sanquin Research (Amsterdam, The Netherlands). Serum samples of diseased donors were purchased from Discovery Life Sciences (Columbus, OH, USA). Furth | Full Paper |
2022 | Pawley, DC;Dikici, E;Deo, SK;Raccamarich, P;Fischl, MA;Alcaide, M;Daunert, S; | Rapid Point-of-Care Test Kit for Bacterial Vaginosis: Detection of Vaginolysin and Clue Cells Using Paper Strips and a Smartphone | Analytical chemistry | 35943181 | Purchased patient samples from Discovery Life Sciences confirmed positive for BV were also tested. All patient samples tested showed elevated levels of VLY, indicating a Save | Full Paper |
2022 | Nuti, S;Zhang, Y;Zerrouki, N;Roach, C;Bänfer, G;Kumar, GL;Manna, E;Diezko, R;Kersch, K;Rüschoff, J;Jasani, B; | High interobserver and intraobserver reproducibility among pathologists assessing PD-L1 CPS across multiple indications | Histopathology | 35993150 | Diezko is an employee of Targos Molecular Pathology GmbH, which performed pharmaceutical industry-sponsored professional training for pathologists and clinical trial Save;Biomarkers and Diagnostics, Oncology, Global Medical and Scientific Affairs, | Full Paper |
2022 | Loibl, S;Huang, CS;Mano, MS;Mamounas, EP;Geyer, CE;Untch, M;Thery, JC;Schwaner, I;Limentani, S;Loman, N;Lübbe, K;Chang, JC;Hatschek, T;Tesarowski, D;Song, C;Lysbet de Haas, S;Boulet, T;Lambertini, C;Wolmark, N; | Adjuvant trastuzumab emtansine in HER2-positive breast cancer patients with HER2-negative residual invasive disease in KATHERINE | NPJ breast cancer | 36117201 | es obtained from the pretreatment primary tumor biopsy material (or residual tumor tissue from definitive surgery post-NAST). HER2 status of all samples was centrally assessed in the same laboratory (Targos Molecular Pathology, GmbH [Kassel, Germany] | Full Paper |
2022 | Seok, Y;Yin, Q;Li, R;Mauk, M;Bai, H;Bau, H; | Manually-operated, slider cassette for multiplexed molecular detection at the point of care | Sensors and Actuators B: Chemical | HCV genotype 1 plasma sample (CV900106678051314DD, 69,000,000 IU/mL) for clinical research was purchased from Discovery life science Inc. (Huntsville, AL, USA) | Full Paper | |
2022 | Singampalli, KL;Li, J;Lillehoj, PB; | Rapid magneto-enzyme-linked immunosorbent assay for ultrasensitive protein detection | Analytica chimica acta | 36038242 | falciparum infection obtained in Uganda under IRB/EC approval for general research use were purchased from Discovery Life Sciences. All human samples were de- Save; Blood samples from donors with P. falciparum infection obtained in Uganda under IRB/ | Full Paper |
2022 | Koehler, VF;Adam, P;Fuss, CT;Jiang, L;Berg, E;Frank-Raue, K;Raue, F;Hoster, E;Knösel, T;Schildhaus, HU;Negele, T;Siebolts, U;Lorenz, K;Allelein, S;Schott, M;Spitzweg, C;Kroiss, M;German Study Group for Rare Malignant Tumors of the Thyroid and Parathyroid Glands, ; | Treatment of RET-Positive Advanced Medullary Thyroid Cancer with Multi-Tyrosine Kinase Inhibitors-A Retrospective Multi-Center Registry Analysis | Cancers | 35884466 | US is an employee of Targos Molecular Pathology, Inc., and received honoraria from or served as an advisory board member at Roche, Novartis Oncology, MSD, BMS, Save Cached | Full Paper |
2022 | Molina, SA;Davies, SJ;Sethi, D;Oh, S;Durand, N;Scott, M;Davies, LC;Wormuth, K;Clarke, D; | Particulates are everywhere, but are they harmful in cell and gene therapies? | Cytotherapy | 36175323 | International Society for Cell & Gene Therapy Process Development, Manufacturing, and Analytics Committee, Vancouver, Canada. Electronic address: sam.molina@gmail.com.++International Society for Cell & Gene Therapy Process Development, Manufacturing, | Full Paper |
2022 | Ren, Z;Chen, S;Qin, X;Li, F;Guo, L; | Study of the roles of cytochrome P450 (CYPs) in the metabolism and cytotoxicity of perhexiline | Archives of toxicology | 36083301 | Perhexiline is a prophylactic antianginal agent developed in the 1970s. Although, therapeutically, it remained a success, the concerns of its severe adverse effects Save;- Primary human hepatocytes (pooled from 10 donors) were obtained from Disc | Full Paper |
2022 | Cheng, LY;Dai, P;Wu, LR;Patel, AA;Zhang, DY; | Direct capture and sequencing reveal ultra-short single-stranded DNA in biofluids | iScience | 36147958 | N = 6 plasma samples from diseased individuals (3 from Type 1 Diabetes (T1D) patients and 3 from Alzheimer’s disease (AD) patients) were acquired from BioIVT’s biorepository and shipped frozen. Animal total blood samples were purchased from Discovery | Full Paper |
2022 | Kemper, K;Gielen, E;Boross, P;Houtkamp, M;Plantinga, T;de Poot, S;Burm, S;Janmaat, M;Koopman, L;van den Brink, E;Rademaker, R;Verzijl, D;Engelberts, P;Satijn, D;Sasser, A;Breij, E; | Mechanistic and pharmacodynamic studies of DuoBody-CD3x5T4 in preclinical tumor models | Life Science Alliance | Dissociated tumor cells (DTCs) were procured from commercial vendors (Prisma Health/KIYATEC Biorepository or Discovery Life Sciences) who maintain strict ethical compliance, including fully | Full Paper | |
2022 | Rüschoff, J;Friedrich, M;Nagelmeier, I;Kirchner, M;Andresen, LM;Salomon, K;Portier, B;Sredni, ST;Schildhaus, HU;Jasani, B;Grzelinski, M;Viale, G; | Comparison of HercepTest™ mAb pharmDx (Dako Omnis, GE001) with Ventana PATHWAY anti-HER-2/neu (4B5) in breast cancer: correlation with HER2 amplification and HER2 low status | Virchows Archiv : an international journal of pathology | 35970977 | in HER2 evaluation, having served over the past 20 years as readers in most of the trastuzumab, pertuzumab, and T-DM1 approval BC studies by Targos GmbH (Kassel, Save;Targos - A Discovery Life Sciences Company, Germaniastraße 7, 34119, Kassel, Germ | Full Paper |
2022 | Suwa, R;Kume, Y;Kawase, M;Chishiki, M;Ono, T;Norito, S;Sato, K;Okamoto, M;Kumaki, S;Nagai, Y;Hosoya, M;Takeda, M;Nishimura, H;Hashimoto, K;Shirato, K; | Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in Japan | Pathogens (Basel, Switzerland) | 35889999 | To confirm amplification specificity, we used pharyngeal, nasopharyngeal, and nasal swabs from Discovery Life Sciences (Los Osos, CA, USA) that were obtained for the Save Cached | Full Paper |
2022 | Rodrigues, D;van Kampen, R;van Bodegraven, AA;Kleinjans, JCS;Jennen, DGJ;de Kok, TM; | Gene expression responses reflecting 5-FU-induced toxicity: Comparison between patient colon tissue and 3D human colon organoids | Toxicology letters | 36183961 | All patients provided written informed consent. Human colon tissue samples were obtained by Boehringer Ingelheim Pharmaceuticals Inc. (Ridgefield, Connecticut, USA) from Discovery Life Sciences (Huntsville, Alabama, USA; formerly, Conversant Biologic | Full Paper |
2022 | Cao, L;Ledeboer, A;Pan, Y;Lu, Y;Meyer, K; | Clinical enrollment assay to detect preexisting neutralizing antibodies to AAV6 with demonstrated transgene expression in gene therapy trials | Gene therapy | 35778500 | Individual human serum samples were purchased from BioIVT (Westbury, NY, USA), Discovery Life Sciences (Los Osos, CA, USA), and Golden West Biologicals (Temecula, CA, USA) | Full Paper |
2022 | Zhang, L;Wang, Y;Homan, KT;Gaudette, SM;McCluskey, AJ;Chan, Y;Murphy, J;Abdalla, M;Nelson, CM;Sun, VZ;Erickson, JE;Knight, HL;Clabbers, A;Sterman, AJS;Mitra, S; | Imaging the Alternatively Spliced D Domain of Tenascin C in a Preclinical Model of Inflammatory Bowel Disease | Molecular imaging and biology | 35906512 | Clinical IBD colon was evaluated using a biotinylated anti-TNC D antibody. Frozen human tissue was obtained from CHTN (National Cancer Institute, Bethesda, MD), Folio Biosciences (Columbus, OH), and UMASS Memorial Gastroenterology (Worcester, MA) | Full Paper |
2022 | Holmberg, JA;Henry, SM;Burnouf, T;Devine, D;Marschner, S;Boothby, TC;Burger, SR;Chou, ST;Custer, B;Blumberg, N;Siegel, DL;Spitalnik, SL; | National Blood Foundation 2021 Research and Development summit: Discovery, innovation, and challenges in advancing blood and biotherapies | Transfusion | 36169155 | consulting firm, and one of our clients is Discovery Life Sciences, an, apheresis services provider. My advice to DLS is the same as what I say in this manuscript. HemaCare Save | Full Paper |
2022 | Edmonds, NL;Flores, SE;Mahmutovic, A;Young, SJ;Mauldin, IS;Slingluff, CL; | CD103 and periplakin are potential biomarkers for response of metastatic melanoma to pembrolizumab | Melanoma research | 36169985 | QualTek provided a modified proportion score indicating the percent of PD-L1 Qualtek also reported an overall estimate of the presence/absence of tumor-infiltrating Save | Full Paper |
2022 | Kandi, S;Savaryn, JP;Ji, QC;Jenkins, GJ; | Use of In-Sample Calibration Curve approach for quantification of peptides with high resolution Mass Spectrometry | Rapid communications in mass spectrometry : RCM | 35940586 | Human Peripheral Blood Mononuclear Cells (hPBMCs) were purchased from Discovery Life Sciences (Newtown, PA, USA) | Full Paper |
2022 | Boillat, MA;Hauser, PC; | CO2-measuring dongle | HardwareX | 35873734 | 11 2.11 Mouser.com semiconductor U4 Sensirion SCD41-D-R1 1 45.66 45.66 Mouser.com CO2-sensor J1 GCT USB3505-30-A-KIT 1 2.35 2.35 Mouser.com Micro B USB 2.0 socket Qualtek 3025033-1 1 2.97 2.97 Mouser.com | Full Paper |
2022 | Schöniger, S;Rüschoff, J; | Mismatch Repair Deficiency and Microsatellite Instability | Encyclopedia | Targos—A Discovery Life Sciences Company, Germaniastrasse 7, 34119 Kassel, Germany | Full Paper | |
2022 | Li, W;Li, Y;Xu, J;Chen, C;Gu, Y;Lu, Z;Li, D;Li, J;Sorg, A;Roberts, C;Mahajan, S;Gallant, M;Taggart, D; | Multimodal Epigenetic Sequencing Analysis (MESA) of Cell-free DNA for Non-invasive Cancer Detection | Research Square | Cohort 1 subjects were recruited at clinical sites within the United States through the ELITE Study (NCT05181826) or were obtained through the following contract research organizations: BioIVT (Westbury, NY), BioOptions (Brea, CA), Discovery Life Sci | Full Paper | |
2022 | Koerner, L;Yang, T;Schmiel, M;Peifer, M;Buettner, R;Pasparakis, M; | NEMO- and RelA-dependent NF-?B Signaling Promotes Small Cell Lung Cancer | bioRxiv | Small cell lung cancer (SCLC) is an aggressive type of lung cancer driven by combined loss of the tumor suppressors RB1 and TP53. SCLC is highly metastatic and Save Cached | Full Paper | |
2022 | Hebda-Bauer, E;Hagenauer, M;Blandino, P;Meng, F;Chitre, A;Ozel, A;Arakawa, K;Flagel, S;Watson, S;Palmer, A;Li, J;Akil, H; | Transcriptional Profiling of the Hippocampus in an F2 Cross of a Genetic Rat Model of Internalizing vs. Externalizing Behavior and Addiction Liability | bioRxiv | HudsonAlpha Discovery provided a variety of metrics for evaluating sample RNA quality, including RNA concentration (RNAconc), RNA integrity number (RIN), the Save Cached | Full Paper | |
2022 | Chitre, A;Hebda-Bauer, E;Blandino, P;Bimschleger, H;Nguyen, K;Maras, P;Li, F;Ozel, A;Polysskaya, O;Cheng, R;Flagel, S;Watson, S;Li, J;Akil, H;Palmer, A; | Genome-Wide Association Study in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like Traits | bioRxiv | For these 20 rats, liver tissue was sent to HudsonAlpha Discovery (Discovery Life Sciences) for HMW genomic DNA extraction and long-range and deep sequencing. Save | Full Paper | |
2022 | Dejanovic, B;Wu, T;Tsai, M;Graykowski, D;Gandham, V;Rose, C;Bakalarski, C;Ngu, H;Wang, Y;Pandey, S;Rezzonico, M;Friedman, B;Edmonds, R;De Mazière, A;Rakosi-Schmidt, R;Singh, T;Klumperman, J;Foreman, O;Chang, M;Xie, L;Sheng, M;Hanson, J; | Complement C1q-dependent excitatory and inhibitory synapse elimination by astrocytes and microglia in Alzheimer’s disease mouse models | Nature Aging | CSF from patients with AD and healthy controls was obtained from Folio Biosciences and Precision Medicine with ethics committee approval and written informed consent | Full Paper | |
2022 | Cady, J;Greytak, E; | Whole-genome sequencing of degraded DNA for investigative genetic genealogy | Forensic Science International: Genetics Supplement Series | Samples were submitted to either HudsonAlpha Discovery or Arbor Biosciences for library preparation and sequencing. For each sample, a small amount of the prepared Save | Full Paper | |
2022 | Newman, L;Useckaite, Z;Rowland, A; | Addressing MISEV guidance using targeted LC?MS/MS: A method for the detection and quantification of extracellular vesicle?enriched and contaminant protein markers from blood | Journal of Extracellular Biology | Serum and plasma samples from patients with non-alcoholic fatty liver disease (NAFLD) were purchased from Discovery Life Sciences (Hunstville, AL, USA). Save | Full Paper | |
2022 | D'ANTONIO, L; | Esposizione a paracetamolo e acido sulfosalicilico: effetti biologici nel bivalve Mytilus spp. | Thesis | Francisca Piedade, Sofia Bio, Bruno Nunes. 2020 Effects of common pharmaceutical drugs | Full Paper | |
2022 | Wagner, RS; | Environmental Factors Affecting Rhizophydiales Sp. Infecting Planktothrix Spp. | Thesis | were then sent to HudsonAlpha Discovery Life Sciences (Hudson, AL USA) for sequencing. Sequencing and QC was done at HudsonAlpha Discovery Life Sciences using Save;- then sent to HudsonAlpha Discovery Life Sciences (Hudson, AL USA) for sequencin | Full Paper | |
2022 | Ferreira Garcia Rodrigues, D; | Gut feelings | maastricht university | Human healthy colon organoids were derived from a tissue biopsy collected from a healthy male donor (67 years old) and kindly provided by Boehringer Ingelheim Pharmaceuticals Inc. (Ridgefield, USA), who purchased tissue biopsies from Discovery Life S | Full Paper | |
2022 | Fang, G; | The use of perfusion MRI using ferumoxytol and small molecular weight gadolinium (Gd) agents to assess response to pembrolizumab in brain metastases and systemic lesions in NSCLC: A comparison of imaging modalities to address brain metastases, pseudoprogression and systemic lesion tumor flare (neuro-check pilot) | Thesis | Tissue samples will be sent to QualTek Molecular Laboratories for PD-L1 biomarker Brain Tissue samples will also be sent to QualTek Molecular Laboratories for PD-1 Save | Full Paper | |
2022 | Isakoff, SJ; | DF/HCC Protocol#: 17-328 TITLE: A Phase 1b Study of Safety and Immune Response to PVX-410 Vaccine Alone and in Combination with Pembrolizumab | webctportal.partners.org | Primary tumor slides will be evaluated for expression of PD-L1 using the Merck Preferred Vendor QualTek according to their standard procedures. A | Full Paper | |
2022 | Frankovi?, J; | Koncerti hrvatskih autora za violon?elo i orkestar | Thesis | Makedonskog teatra te je osniva? komornog ansambla za suvremenu glazbu „Sveta Sofia“. Bio je jedan od najplodnijih jugoslavenskih skladatelja srednje generacije te se okušao u svim stvarala?kim sferama | Full Paper | |
2022 | Yang, SYC; | The Molecular Determinants of Response to Immune Checkpoint Therapy in Solid Tumors | Thesis | on 4-5?m sections mounted on positively charged ProbeOn slides (QualTek, Goleta, CA). QualTek provided a modified proportion score (MPS) indicating the proportion of Save | Full Paper | |
2022 | Shen, Y;Guo, K;Ma, A;Huang, Z;Du, J;Chen, J;Lin, Q;Wei, C;Wang, Z;Zhang, F;Zhang, J;Lin, W;Feng, N;Ma, W; | Mitochondrial toxicity evaluation of traditional Chinese medicine injections with a dual in vitro approach | Frontiers in pharmacology | 36408232 | and Health Sciences, Wuyi University, Jiangmen, China 3 Antibody Engineering Laboratory, School of Life Science & Technology, China Pharmaceutical University, Nanjing, China Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States Review | Full Paper |
2022 | Tiu, C;Lopez, J; | Lisavanbulin. Microtubule destabilizer (tubulin polymerization inhibitor), Tumor checkpoint controller, Treatment of advanced glioblastoma multiforme | Drugs of the Future | Assessment of 108 clinical trial samples from NCT02490800, using a CE-marked immunohistochemistry Clinical Trial Assay (CTA; Targos Molecular Pathology GmbH, Save | Full Paper | |
2022 | Drapkin, R;Jung, E;Bradshaw, C;Demars, L;Mosher, R; | EP014/#408?Evaluation of NAPI2B expression in a well annotated longitudinal tissue series of ovarian serous carcinomas | E-Posters | IHC analyses were performed by QualTek Molecular Laboratories (Discovery Life Sciences). Editorial support for this poster was provided by BluPrint Oncology. Save | Full Paper | |
2022 | Korner, LK; | Cell death and inflammation in skin homeostasis and small cell lung cancer | Thesis | R.B. is an employee of Targos Molecular Pathology. The other authors declare no competing interests | Full Paper | |
2022 | Weng, H;Huang, F;Yu, Z;Chen, Z;Prince, E;Kang, Y;Zhou, K;Li, W;Hu, J;Fu, C;Aziz, T;Li, H;Li, J;Yang, Y;Han, L;Zhang, S;Ma, Y;Sun, M;Wu, H;Zhang, Z;Wunderlich, M;Robinson, S;Braas, D;Hoeve, JT;Zhang, B;Marcucci, G;Mulloy, JC;Zhou, K;Tao, HF;Deng, X;Horne, D;Wei, M;Huang, H;Chen, J; | The m6A reader IGF2BP2 regulates glutamine metabolism and represents a therapeutic target in acute myeloid leukemia | Cancer cell | 36306790 | Bone marrow (BM) cells were collected from 5- to 7-week-old wild-type, Mettl14fl/flCreERT, or Igf2bp2fl/fl mice, and BM progenitor (HSPC, i.e., Lin- or c-kit+) cells were enriched with the Mouse Lineage Cell Depletion Kit or the CD117 microbeads (Mil | Full Paper |
2022 | Wei, J;Montalvo-Ortiz, W;Yu, L;Krasco, A;Olson, K;Rizvi, S;Fiaschi, N;Coetzee, S;Wang, F;Ullman, E;Ahmed, HS;Herlihy, E;Lee, K;Havel, L;Potocky, T;Ebstein, S;Frleta, D;Khatri, A;Godin, S;Hamon, S;Brouwer-Visser, J;Gorenc, T;MacDonald, D;Hermann, A;Chaudhry, A;Sirulnik, A;Olson, W;Lin, J;Thurston, G;Lowy, I;Murphy, AJ;Smith, E;Jankovic, V;Sleeman, MA;Skokos, D; | CD22-targeted CD28 bispecific antibody enhances antitumor efficacy of odronextamab in refractory diffuse large B cell lymphoma models | Science translational medicine | 36350988 | For the NALM6-luc tumor experiments, 12-week-old female immunodeficient NSG mice (Jackson Laboratory, 005557) were engrafted with 4 × 10 6 human PBMCs (ReachBio), and Save | Full Paper |
2022 | Devoucoux, M;Fort, V;Khelifi, G;Xu, J;Alerasool, N;Galloy, M;Wong, N;Bourriquen, G;Fradet-Turcotte, A;Taipale, M;Hope, K;Hussein, SMI;Côté, J; | Oncogenic ZMYND11-MBTD1 fusion protein anchors the NuA4/TIP60 histone acetyltransferase complex to the coding region of active genes | Cell reports | 35705031 | Recombinant Mouse TPO PeproTech Cat# AF-315-14 Recombinant Mouse IL-3 R&D Systems Cat# 403-ML-050 Recombinant Mouse IL-6 R&D Systems Cat# 406-ML-025 ColonyGEL, Mouse Complete Medium ReachBio Cat# 1202 SFEM II Media STEMCELL Technologies Cat# 09655 | Full Paper |
2022 | Peterson, CW;Venkataraman, R;Reddy, SS;Pande, D;Enstrom, MR;Radtke, S;Humbert, O;Kiem, HP; | Intracellular RNase activity dampens zinc finger nuclease-mediated gene editing in hematopoietic stem and progenitor cells | Molecular therapy. Methods & clinical development | 34977270 | nces, San José, CA) into D-PBS, followed by total genomic DNA extraction and NGS. Colony-forming assays ZFN- or CRISPR/Cas9-treated NHP cells (1.2 × 103) were plated in 3.6 mL of ColonyGEL 1402 (ReachBio, Seattle, WA). For colony-forming assays util | Full Paper |
2022 | Liu, L;Li, H;Patterson, AM;Plett, PA;Sampson, CH;Mohammad, KS;Capitano, ML;Singh, P;Yao, C;Orschell, CM;Pelus, LM; | Upregulation of SIRT1 Contributes to dmPGE2-dependent Radioprotection of Hematopoietic Stem Cells | Stem cell reviews and reports | 35318613 | Colony forming cells were analyzed as described [20]. Total nucleated BM cells from non-irradiated mice were plated at 2 × 104 per dish in Mouse Complete Colony Gel media (Reach Bio, Seattle, WA). BM cells from irradiated C57BL/6 mice were plated at | Full Paper |
2022 | Canals Hernaez, D;Hughes, MR;Li, Y;Mainero Rocca, I;Dean, P;Brassard, J;Bell, EM;Samudio, I;Mes-Masson, AM;Narimatsu, Y;Clausen, H;Blixt, O;Roskelley, CD;McNagny, KM; | Targeting a Tumor-Specific Epitope on Podocalyxin Increases Survival in Human Tumor Preclinical Models | Frontiers in oncology | 35600398 | Linker stability was independently assessed by ReachBio Research Labs (RRL, www.reachbio.com) using an in vitro neutrophil differentiation killing assay, where extracellular Save;Emer Clarke at ReachBio Research Labs (www.reachbio.com) for providin | Full Paper |
2022 | Lefavor, R;Clarke, E; | The Effect of Various Stimulants on Cytokine Secretion Profiles in Freshly Isolated Peripheral Blood Mononuclear Cells (PBMC) | The Journal of Immunology | 1ReachBio Research Labs++1ReachBio Research Labs | Full Paper | |
2022 | Gu, Q;Palani, CD;Smith, A;Li, B;Amos-Abanyie, EK;Ogu, U;Lu, L;Pace, BS;Starlard-Davenport, A; | MicroRNA29B induces fetal hemoglobin via inhibition of the HBG repressor protein MYB in vitro and in humanized sickle cell mice | Frontiers in medicine | 36507536 | kville, MD, USA) was used alone or co-transfected with MIR29B. Erythroid differentiation of human CD34+ stem cells and microRNA29B co-electroporation with MYB DNA Human bone marrow CD34+ stem cells (ReachBio, Seattle, WA, USA) were cultured in a mod | Full Paper |
2022 | Balk, R;Esper, A;Martin, G;Miller, R;Lopansri, B;Burke, J;Levy, M;Opal, S;Rothman, R;D'Alessio, F;Sidhaye, V;Aggarwal, N;Greenberg, J;Yoder, M;Patel, G;Gilbert, E;Parada, J;Afshar, M;Kempker, J;van der Poll, T;Schultz, M;Scicluna, B;Klein Klouwenberg, P;Liebler, J;Blodget, E;Kumar, S;Navalkar, K;Yager, T;Sampson, D;Kirk, J;Cermelli, S;Davis, R;Brandon, R; | Validation of SeptiCyte RAPID to discriminate sepsis from non-infectious systemic inflammation | medRxiv | The authors thank the clinical study coordinators of the NEPTUNE study: Joyce D. Brown (Rush University), Liliacna Jara (University of Southern California), Leona Wells and Maya C. Whaley (Grady Memorial Hospital). The authors also thank the laborato | Full Paper | |
2022 | Vujovic, A;de Rooij, L;Chahi, A;Chen, H;Yee, B;Loganathan, S;Liu, L;Chan, D;Tajik, A;Tsao, E;Moreira, S;Joshi, P;Xu, J;Wong, N;Zandi, S;Aigner, S;Dick, J;Minden, M;Schramek, D;Yeo, G;Hope, K; | A two-step in vivo CRISPR screen unveils pervasive RNA binding protein dependencies for leukemic stem cells and identifies ELAVL1 as a therapeutic target | bioRxiv | Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio), supplemented with DMSO or 1.1uM DHTS. Colonies were scored on days 10-14. Human CB samples were | Full Paper | |
2022 | Keyvani Chahi, A; | CHARACTERIZING THE ROLE OF THE TRANSCRIPTION FACTOR PLAG1 IN HUMAN HEMATOPOIETIC STEM AND PROGENITOR CELLS | Thesis | Rapamycin 50nM Cedarlane/MedChemExpr ess HY-10219 ColonyGEL™ 1102 1X Reachbio 1102 Sterile, Blunt-End Needles, 16 Gauge with Luer Lock NA StemCell Technologies 28110 | Full Paper | |
2022 | Stuart, WR; | Inhibition of Embryonic Ectoderm Development as a Novel Mechanism for Fetal Hemoglobin Induction in Sickle Cell Disease | Thesis | Gating and analysis were performed on FlowJo. Single cells were gated using FSC H vs FSC A. CD235a vs CD71 double positive cells were used for HbF analysis through recommendation from ReachBio as these are the hemoglobin expressing cells within the p | Full Paper | |
2021 | Schneider, BP;Jiang, G;Ballinger, TJ;Shen, F;Chitambar, C;Nanda, R;Falkson, C;Lynce, FC;Gallagher, C;Isaacs, C;Blaya, M;Paplomata, E;Walling, R;Daily, K;Mahtani, R;Thompson, MA;Graham, R;Cooper, ME;Pavlick, DC;Albacker, LA;Gregg, J;Solzak, JP;Chen, YH;Bales, CL;Cantor, E;Hancock, BA;Kassem, N;Helft, P;O'Neil, B;Storniolo, AMV;Badve, S;Miller, KD;Radovich, M; | BRE12-158: A Postneoadjuvant, Randomized Phase II Trial of Personalized Therapy Versus Treatment of Physician's Choice for Patients With Residual Triple-Negative Breast Cancer | Journal of clinical oncology : official journal of the American Society of Clinical Oncology | 34910554 | Speakers' Bureau: Genomic Health, Targos Molecular Pathology, Agilent | Full Paper |
2021 | Vergote, I;González-Martín, A;Ray-Coquard, I;Harter, P;Colombo, N;Pujol, P;Lorusso, D;Mirza, MR;Brasiuniene, B;Madry, R;Brenton, JD;Ausems, MGEM;Büttner, R;Lambrechts, D;European experts’ consensus group, ; | European experts consensus: BRCA/homologous recombination deficiency testing in first-line ovarian cancer | Annals of oncology : official journal of the European Society for Medical Oncology | 34861371 | Testifying Advisor for MSD in GBA-assessment for Pembrolizumab, Advisor for Durvalumab. Co-Founder and CSO for Targos Molecular Pathology, Kassel/Germany until April 2021. DL: Consulting (2019-2021): AstraZeneca (2019-2021); BMS (2019); | Full Paper |
2021 | Voors-Pette, C;Aalders, W;Brill, M;Ferrari, D;Annis, A;Steidl, U;Aivado, M;Vukovic, V; | 1791P A phase I pharmacology study of the dual MDMX/MDM2 inhibitor, ALRN 6924, in healthy volunteers | Annals of Oncology | We thank the subjects who participated in these studies; Discovery Life Sciences in Newtown, PA; and Eric Smith for graphical assistance | Full Paper | |
2021 | Han, GR;Jang, H;Ki, H;Lee, H;Kim, MG; | Reagent Filming for Universal Point-of-Care Diagnostics | Small methods | 34928024 | A/B were purchased from Discovery Life Sciences (Los Osos, CA). T | Full Paper |
2021 | de Klerk, LK;Patel, AK;Derks, S;Pectasides, E;Augustin, J;Uduman, M;Raman, N;Akarca, FG;McCleary, NJ;Cleary, JM;Rubinson, DA;Clark, JW;Fitzpatrick, B;Brais, LK;Cavanaugh, ME;Rode, AJ;Jean, MG;Lizotte, PH;Nazzaro, MJ;Severgnini, M;Zheng, H;Fuchs, CS;Enzinger, PC;Bass, AJ; | Phase II study of pembrolizumab in refractory esophageal cancer with correlates of response and survival | Journal for immunotherapy of cancer | 34593617 | PD-L1 immunohistochemistry was performed on pretreatment FFPE tissue slides using a laboratory developed assay (QualTek Molecular Laboratories, Newtown, Pennsylvania, USA) with the anti-PD-L1 22C3 antibody (Merck & Co, Palo Alto, California, USA). | Full Paper |
2021 | Vowell, K;Conner, M;Perrin, F;Bojczuk, P;Hance, K;Roth, I;Donahue, C;Smothers, J;Waight, J; | 662 Dissecting the CD226 immune axis in the tumor microenvironment using CyTOF-based high-dimensional immunophenotyping | Journal for ImmunoTherapy of Cancer | Ethics Approval All samples were purchased from Discovery Life Sciences (DLS). DLS represents and warrants that it has ownership of all Products available for sale and has properly obtained, where required under HHS/OHRP 45 CFR 46.102 (d) (f), IRB | Full Paper | |
2021 | Binnewies, M;Pollack, JL;Rudolph, J;Dash, S;Abushawish, M;Lee, T;Jahchan, NS;Canaday, P;Lu, E;Norng, M;Mankikar, S;Liu, VM;Du, X;Chen, A;Mehta, R;Palmer, R;Juric, V;Liang, L;Baker, KP;Reyno, L;Krummel, MF;Streuli, M;Sriram, V; | Targeting TREM2 on tumor-associated macrophages enhances immunotherapy | Cell reports | 34686340 | Human tumor samples used for both flow cytometry and single-cell RNA sequencing were acquired from Discovery Life Sciences. 49 human tumor samples were used for flow cytometric analysis of immune composition and TREM2 surface levels. 10 human tumor | Full Paper |
2021 | Ma, J;Tan, X;Kwon, Y;Delgado, ER;Zarnegar, A;DeFrances, MC;Duncan, AW;Zarnegar, R; | A Novel Humanized Model of NASH and Its Treatment With META4, A Potent Agonist of MET | Cellular and molecular gastroenterology and hepatology | 34756982 | Background & Aims Nonalcoholic fatty liver disease is a frequent cause of hepatic dysfunction and is now a global epidemic. This ailment can progress to an advanced form called nonalcoholic steatohepatitis (NASH) and end-stage liver disease. Curren | Full Paper |
2021 | Bassez, A;Vos, H;Van Dyck, L;Floris, G;Arijs, I;Desmedt, C;Boeckx, B;Vanden Bempt, M;Nevelsteen, I;Lambein, K;Punie, K;Neven, P;Garg, AD;Wildiers, H;Qian, J;Smeets, A;Lambrechts, D; | A single-cell map of intratumoral changes during anti-PD1 treatment of patients with breast cancer | Nature medicine | 33958794 | combined with an intensity score, which represents the average intensity of positive tumor cells (0, none; 1, weak, 2, intermediate; 3, strong). PD-L1 (Merck 22C3 antibody) immunohistochemistry was performed by Qualtek Molecular Laboratories as per a | Full Paper |
2021 | Yuki, S;Nakamura, Y;Taniguchi, H;Denda, T;Nishina, T;Hamamoto, Y;Hara, H;Esaki, T;Kawakami, H;Takashima, A;Satoh, T;Sunakawa, Y;Masuishi, T;Shinozaki, E;Moriwaki, T;Miki, I;Shitara, K;Yoshino, T; | Expression of PD-L1 and PD-L2 in colorectal cancer (CRC): A post-hoc integrated analysis of SCRUM-Japan GI-SCREEN CRC. | Journal of Clinical Oncology | Patients (Pts) with MSI-H or BRAF V600E mutant tumors were prioritized to be included. PD-L1 (22C3) and PD-L2 expressions were centrally assessed using immunohistochemical assays at QualTek and NeoGenomics, respectively | Full Paper | |
2021 | Ho, CSL;Tüns, AI;Schildhaus, HU;Wiesweg, M;Grüner, BM;Hegedus, B;Schuler, M;Schramm, A;Oeck, S; | HER2 mediates clinical resistance to the KRASG12C inhibitor sotorasib, which is overcome by co-targeting SHP2 | European journal of cancer (Oxford, England : 1990) | 34715459 | HUS is an employee of Targos Molecular Pathology, Inc.; received research grants (outside this study) from Novartis Oncology; and received honoraria and advisory board services from MSD, BMS, Roche Pharma, Novartis Oncology, ZytoVision, Eisai and P | Full Paper |
2021 | Israeli, S;Golden, A;Atalig, M;Mekki, N;Rais, A;Storey, H;Barbouche, MR;Peck, R; | A Novel Point-of-Care Rapid Diagnostic Test for Screening Individuals for Antibody Deficiencies | Journal of clinical immunology | 34839430 | These thirty-three specimens were acquired from Discovery Life Sciences (Los Osos, CA, USA) with IgG concentration characterized by Siemens BN II System nephelometer (Siemens Healthcare GmbH, Erlangen, Germany). IgG levels in the chosen samples Sa | Full Paper |
2021 | Cheng, Y;Hall, TR;Xu, X;Yung, I;Souza, D;Zheng, J;Schiele, F;Hoffmann, M;Mbow, ML;Garnett, JP;Li, J; | Targeting uPA-uPAR interaction to improve intestinal epithelial barrier integrity in inflammatory bowel disease | EBioMedicine | 34933179 | anoids isolation and culture Isolation and culture of primary intestinal organoids was performed as previously described.2223 Briefly, human small intestinal tissues (obtained from Coversant Bio, now Discovery Life Sciences, https://www.dls.com/biosp | Full Paper |
2021 | Cheng, L;Creasy, T;Pilataxi, F;Greenlees, L;Vence, L;Sridhar, S;Streicher, K; | Effects of combination treatment with durvalumab plus tremelimumab on the tumor microenvironment in non-small-cell lung carcinoma | Cancer immunology, immunotherapy : CII | 34623465 | Frozen NSCLC tumor digests (untreated patients, three squamous cell carcinomas and one adenocarcinoma) were purchased from Discovery Life Sciences and cultured in 96-well plates in a medium containing low-dose interleukin 2 (IL-2) plus 20 µg/mL D a | Full Paper |
2021 | Bröske, AE;Korfi, K;Belousov, A;Wilson, S;Ooi, CH;Bolen, CR;Canamero, M;Alcaide, EG;James, I;Piccione, EC;Carlile, DJ;Dimier, N;Umaña, P;Bacac, M;Weisser, M;Dickinson, M; | Pharmacodynamics and molecular correlates of response to glofitamab in relapsed/refractory non-Hodgkin lymphoma | Blood advances | 34941996 | Biopsies were sent to the central pathology laboratory (Targos Molecular Pathology GmbH, Kassel, Germany) and fresh tissues were embedded on receipt. All biopsies were quality checked by a pathologist on hematoxylin and eosin (H&E) staining | Full Paper |
2021 | Howard, FM;Villamar, D;He, G;Pearson, AT;Nanda, R; | The emerging role of immune checkpoint inhibitors for the treatment of breast cancer | Expert opinion on investigational drugs | 34569400 | PD-L1 status was initially assessed with the QualTek PD-L1 assay and subsequently with the Dako 22C3 PD-L1 assay (with With re-analysis of PD-L1 status, seven patients who had tested positive with the QualTek assay were negative by the 22C3 assay | Full Paper |
2021 | Manos, J;Preiss, C;Venkat, N;Tamm, J;Reinhardt, P;Kwon, T;Wu, J;Winter, A;Jahn, T;Yanamandra, K;Titterton, K;Karran, E;Langlois, X; | UNCOVERING SPECIFICITY OF ENDOGENOUS TAU AGGREGATION IN A HUMAN IPSC-NEURON TAU SEEDING MODEL | iScience | (Braak stage V/VI) and non-AD (Braak stage 0/I) male and female donors used in preparing sarkosyl-insoluble tau seeds were generously provided by the Massachusetts Alzheimer's Disease Research Center or purchased from Folio (now Discovery Life Scie | Full Paper | |
2021 | Da Mesquita, S;Papadopoulos, Z;Dykstra, T;Brase, L;Farias, FG;Wall, M;Jiang, H;Kodira, CD;de Lima, KA;Herz, J;Louveau, A;Goldman, DH;Salvador, AF;Onengut-Gumuscu, S;Farber, E;Dabhi, N;Kennedy, T;Milam, MG;Baker, W;Smirnov, I;Rich, SS;Dominantly Inherited Alzheimer Network, ;Benitez, BA;Karch, CM;Perrin, RJ;Farlow, M;Chhatwal, JP;Holtzman, DM;Cruchaga, C;Harari, O;Kipnis, J; | Meningeal lymphatics affect microglia responses and anti-Aβ immunotherapy | Nature | 33911285 | FC-404-2001) cartridges. Processing of total RNA extracted from mouse meningeal LECs (including linear RNA amplification and cDNA library generation) and RNA-seq was performed by HudsonAlpha Genomic Services Laboratory. For bulk RNA-seq experiments w | Full Paper |
2021 | Matusz-Fisher, A;Tan, AR; | Combination of HER2-targeted agents with immune checkpoint inhibitors in the treatment of HER2-positive breast cancer | Expert opinion on biological therapy | 34806498 | PD-L1-positivity was defined as staining in ≥ 1% of tumor cells or any staining in the stroma using the QualTek immunohistochemistry (IHC) assay. Of note, the assay was changed to the Dako IHC 22C3 pharmDx Q 2 Solutions assay mid-trial due to the p | Full Paper |
2021 | Luk, KC;Gersch, J;Harris, BJ;Holzmayer, V;Mbanya, D;Sauleda, S;Rodgers, MA;Cloherty, G; | More DNA and RNA of HBV SP1 splice variants are detected in genotypes B and C at low viral replication | Scientific reports | 34903774 | genotyping through the Abbott Global Surveillance Program38. All other plasma specimens sourced from France, Germany, Italy, Spain and Vietnam with known viral loads and genotypes were purchased from Discovery Life Sciences (Huntsville, AL). HBV DNA | Full Paper |
2021 | Connolly, P;Stapleton, S;Mosoyan, G;Fligelman, I;Tonar, YC;Fleming, F;Donovan, MJ; | Analytical validation of a multi-biomarker algorithmic test for prediction of progressive kidney function decline in patients with early-stage kidney disease | Clinical proteomics | 34789168 | one specimens collected from patients receiving treatment with biologic therapeutic TNF-α inhibitors used to treat rheumatoid and psoriatic arthritis and other auto-immune diseases were sourced from Discovery Life Sciences (Huntsville, AL). Specimens | Full Paper |
2021 | Jöhrens, K;Rüschoff, J; | The Challenge to the Pathologist of PD-L1 Expression in Tumor Cells of Non-Small-Cell Lung Cancer-An Overview | Current oncology (Toronto, Ont.) | 34940076 | Institute of Pathology, Carl Gustav Carus University Hospital Dresden, 01307 Dresden, Germany.++Targos Molecular Pathology GmbH, 34119 Kassel, Germany | Full Paper |
2021 | Hou, X;Wang, G;Fan, W;Chen, X;Mo, C;Wang, Y;Gong, W;Wen, X;Chen, H;He, D;Mo, L;Jiang, S;Ou, M;Guo, H;Liu, H; | T-cell receptor repertoires as potential diagnostic markers for patients with COVID-19 | International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases | 34688948 | ad recovered from COVID-19. In the COVID-19-BWNW group, whole blood samples were collected at Bloodworks Northwest (Seattle, WA, USA). In the COVID-19-DLS group, whole blood samples were collected at Discovery Life Sciences (Huntsville, AL, USA). In | Full Paper |
2021 | Brosius, C;Caron, K;Sosnoff, C;Blount, B;Wang, L; | Rapid Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method to Measure Cannabinoids in Bronchoalveolar-Lavage Fluid of Patients with e-Cigarette, or Vaping, Product Use-Associated Lung Injury | ACS Omega | BAL pools were created using anonymous BAL samples acquired commercially from Discovery Life Sciences (Huntsville, AL). They were shipped frozen on dry ice and then stored in −70 C freezers until analyzed. Ten individual BAL fluids were screened fo | Full Paper | |
2021 | Sun, Q;Pastor, L;Du, J;Powell, MJ;Zhang, A;Bodmer, W;Wu, J;Zheng, S;Sha, MY; | A novel xenonucleic acid-mediated molecular clamping technology for early colorectal cancer screening | PloS one | 34610014 | give an average DNA fragment length of about 150 bp, which mimics the size of cfDNA fragments, justifying their use as cfDNA references. Except for the 10 CRC whole blood samples were purchased from Discovery Life Sciences (Huntsville, AL, US), All | Full Paper |
2021 | Pound, H;Gann, E;Wilhelm, S; | A comparative study of metatranscriptomic assessment methods to characterize Microcystis blooms | Limnology and Oceanography: Methods | Extracted RNA was processed using a Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus and then 50-million 100-bp paired-end reads were generated on the Illumina NovaSeq platform at Hudson Alpha Discovery Life Sciences (Huntsville, Sa | Full Paper | |
2021 | Miao, LY;Kim, HJ;Whitlatch, K;Jaiswal, D;Navarro, A;Egan, R;Olivo, PD; | A rapid homogenous bioassay for detection of thyroid-stimulating antibodies based on a luminescent cyclic AMP biosensor | Journal of immunological methods | 34871593 | TSI-positive serum samples were a kind gift of Dr. George Kahaly. Anti-thyroid peroxidase (TPO) antibody-positive human serum samples and nonthyroidal autoimmune disease state human serum samples were obtained from Discovery Life Science (Los Osos, C | Full Paper |
2021 | Nuovo, G;Suster, D;Tili, E;Awad, H;Magro, C; | A Standardization Protocol for the In Situ Detection of SARS-CoV2 RNA and Proteins | Applied Immunohistochemistry & Molecular Morphology | vid MD‡; Tili, Esmerina PhD§; Awad, Hamdy MD§; Magro, Cynthia MD∥ Author Information __ *Ohio State University Comprehensive Cancer Center §Ohio State University Medical Center, Columbus †Discovery Life Sciences, Powell, OH ‡Department of Patholo | Full Paper | |
2021 | Nguyen, M;Gary, J;Sonoda, J;Low, M;Ring, C;Bishop, J; | Feasibility of Lateral Flow Assays for Urinary Biomarkers to Nicotine Delivery Products | OSF PREPRINTS2021 | A prospective collection of 212 individual urine specimens from users and non-nicotine users was completed between July 2020 and April 2021 by Discovery Life Sciences (Los Osos, CA). All enrollees selfidentified as non-users (112 samples) or single | Full Paper | |
2021 | Zhang, L;Pohl, C;Homan, K;Sun, V;Gaudette, S;Nelson, C;Erickson, J;Knight, H;Bose, S;Lynch, G;McRae, B;Sterman, A;Mitra, S; | Imaging the Alternatively Spliced D Domain of Tenascin C in Preclinical Models of Inflammatory Bowel Disease | Research Square | Clinical IBD and diseased cynomolgus macaque colon were evaluated using a biotinylated anti-TNC D antibody. Frozen human tissue was obtained from CHTN (National Cancer Institute, Bethesda, MD), Folio Biosciences (Columbus, OH), and UMASS Memorial Gas | Full Paper | |
2021 | Kehl, F;Cretu, V;Willis, P; | Open-source lab hardware: Driver and temperature controller for high compliance voltage, fiber-coupled butterfly lasers | HardwareX | We recommend applying 12 VDC at the voltage input (VIN), eg, by connecting a wall adapter with sufficient power such as the Qualtek QFWB-65-12-US01, which can provide up to 5 A at 12 VDC. The input voltage is directly fed into a voltage regulator ( | Full Paper | |
2021 | Lockhart, JW;Jacobs, AZ; | Scientific Argument with Supervised Learning | NeurIPS 2021 AI for Science Workshop | ML for scientific discovery Life sciences often advance through creating and testing taxonomies or classifications of entities. Statistical methods to evaluate the theorized differences between groups offer the opportunity to validate such classifi | Full Paper | |
2021 | Hair, J;Robinson, MJ;Wilkinson, RW;Dovedi, SJ; | Deep phenotyping of surface stimulatory and inhibitory co-receptors on cancer-resident T and NK cells reveals cell subsets within the tumor-reactive CTL population that are uniquely defined by NKG2A expression | SLAS Discovery | Disaggregated tumor cells (DTC; Discovery Life Sciences), which are a single-cell suspension of enzymatically-dissociated tumor tissue, from NSCLC (n=13), RC (n=10) and melanoma (n=10) patients were used. Detailed per-patient disease classification | Full Paper | |
2021 | Edmund, M; | RISING STARS | Quality Progress | Quality associate, lead auditor, Discovery Life Sciences (DLS), Powell, OH | Full Paper | |
2021 | Valle, J;Kelley, R;Nervi, B;Oh, D;Zhu, A; | Biliary tract cancer | The Lancet | In KEYNOTE-028 PD-L1 positivity was defined as membranous PD-L1 expression in at least 1% of tumour and associated inflammatory cells, or positive staining in stroma (immunohistochemistry assay, QualTek, Goleta, CA, USA), and all patients were PD-L1 | Full Paper | |
2021 | Tiu, C;Derby, S;Haris, N;Welsh, L;Stansfeld, A;Hundsberger, T;Roth, P;König, F;Eisner, J;Kleinschmidt, M;Anderson, S;Bachmann, F;Lane, H;Engelhardt, M;Kaindl, T;Litherland, K;Stan, A;Evans, T;Plummer, E;Lopez, J; | The potential utility of end-binding protein 1 (EB1) as response-predictive biomarker for lisavanbulin: A phase 2 study of lisavanbulin (BAL101553) in adult patients with recurrent glioblastoma. | Journal of Clinical Oncology | Gallen, Switzerland; Department of Neurology, University Hospital Zurich, Zurich, Switzerland; Targos Molecular Pathology GmbH, Kassel, Germany; GeneCentric Therapeutics, Inc., Research Triangle Park, NC; Basilea Pharmaceutica International Ltd., Bas | Full Paper | |
2021 | Skowronska, M;Tiu, C;Tzankov, A;König, F;Lewis, J;Vivanco, I;Kleinschmidt, M;Beebe, K;Anderson, S;Bachmann, F;Engelhardt, M;Lane, H;Kaindl, T;Stan, A;Plummer, E;Evans, T;Zlobec, I;Lopez, J; | Expression of end-binding protein 1 (EB1), a potential response-predictive biomarker for lisavanbulin, in glioblastoma and various other solid tumor types. | Journal of Clinical Oncology | for articles by this author. Show More Institute of Pathology, University of Bern, Bern, Switzerland; The Royal Marsden NHS Foundation Trust, Sutton, United Kingdom; Institute of Pathology, University Hospital Basel, Basel, Switzerland; Targos Molecu | Full Paper | |
2021 | Pratto, F;Brick, K;Cheng, G;Lam, KG;Cloutier, JM;Dahiya, D;Wellard, SR;Jordan, PW;Camerini-Otero, RD; | Meiotic recombination mirrors patterns of germline replication in mice and humans | Cell | 34260899 | Human testicular biopsy from cancer patient (Normal adjacent tissue) Folio Biosciences now Discovery Life Sciences biospecimen bank https://www.dls.com | Full Paper |
2021 | Scoazec, J;Bauchet, A;Adam, J;Rochaix, P;MacGrogan, G;Kim, J;Nuciforo, P;López-Rios, F;Frisman, D;Cesarone, G;Bernstein, S;Lee, J;Henry, C;Ozoux, M;Lefebvre, A; | 1313P Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) tumor expression in a phase I/II study of tusamitamab ravtansine (SAR408701) in patients (pts) with advanced non-small cell lung cancer (NSCLC) | Annals of Oncology | articles by this author. D. Frisman D. Frisman. Affiliations Discovery Life Sciences, Goleta, CA, USA. Search for articles by this author. G. Cesarone G. Cesarone. Affiliations Discovery Life Sciences, Goleta, CA, USA. Search for articles | Full Paper | |
2021 | Salgado, R;Peg, V;Rüschoff, J;Vincent-Salomon, A;Castellano, I;Perner, S;Van de Vijver, K;Quinn, CM;Varga, Z; | Gene expression signatures for tailoring adjuvant chemotherapy of luminal breast cancer: the pathologists' perspective | Annals of oncology : official journal of the European Society for Medical Oncology | 34461263 | Department of Pathology, GZA-ZNA Hospitals, Antwerp, Belgium; Division of Research, Peter MacCallum Cancer Centre, Melbourne, Australia. Electronic address: roberto@salgado.be.++Universidad Autónoma de Barcelona, Barcelona, Spain; Department of Patho | Full Paper |
2021 | Keenan, TE;Guerriero, JL;Barroso-Sousa, R;Li, T;O'Meara, T;Giobbie-Hurder, A;Tayob, N;Hu, J;Severgnini, M;Agudo, J;Vaz-Luis, I;Anderson, L;Attaya, V;Park, J;Conway, J;He, MX;Reardon, B;Shannon, E;Wulf, G;Spring, LM;Jeselsohn, R;Krop, I;Lin, NU;Partridge, A;Winer, EP;Mittendorf, EA;Liu, D;Van Allen, EM;Tolaney, SM; | Molecular correlates of response to eribulin and pembrolizumab in hormone receptor-positive metastatic breast cancer | Nature communications | 34548479 | Tumors from 65/88 (74%) patients underwent PD-L1 testing assessed centrally by QualTek using the 22C3 antibody | Full Paper |
2021 | Brägelmann, J;Lorenz, C;Borchmann, S;Nishii, K;Wegner, J;Meder, L;Ostendorp, J;Ast, DF;Heimsoeth, A;Nakasuka, T;Hirabae, A;Okawa, S;Dammert, MA;Plenker, D;Klein, S;Lohneis, P;Gu, J;Godfrey, LK;Forster, J;Trajkovic-Arsic, M;Zillinger, T;Haarmann, M;Quaas, A;Lennartz, S;Schmiel, M;D'Rozario, J;Thomas, ES;Li, H;Schmitt, CA;George, J;Thomas, RK;von Karstedt, S;Hartmann, G;Büttner, R;Ullrich, RT;Siveke, JT;Ohashi, K;Schlee, M;Sos, ML; | MAPK-pathway inhibition mediates inflammatory reprogramming and sensitizes tumors to targeted activation of innate immunity sensor RIG-I | Nature communications | 34535668 | M.L.S. is a founder and shareholder of PearlRiver Bio (now part of Centessa Pharmaceuticals) and received consulting honoraria from PearlRiver Bio. M.L.S. receives research funding from PearlRiver Bio and Novartis. R.B. is an employee of Targos Molec | Full Paper |
2021 | Ny, L;Jespersen, H;Karlsson, J;Alsén, S;Filges, S;All-Eriksson, C;Andersson, B;Carneiro, A;Helgadottir, H;Levin, M;Ljuslinder, I;Olofsson Bagge, R;Sah, VR;Stierner, U;Ståhlberg, A;Ullenhag, G;Nilsson, LM;Nilsson, JA; | The PEMDAC phase 2 study of pembrolizumab and entinostat in patients with metastatic uveal melanoma | Nature communications | 34453044 | st was used to assess associations between immunohistochemical analyses (PD-L1 and TILs) and clinical benefit. Immunohistochemical analyses Tumor PD-L1 testing was performed at a central laboratory (QualTek Molecular Laboratories, Newtown, Pennsylva | Full Paper |
2021 | Cindy Yang, SY;Lien, SC;Wang, BX;Clouthier, DL;Hanna, Y;Cirlan, I;Zhu, K;Bruce, JP;El Ghamrasni, S;Iafolla, MAJ;Oliva, M;Hansen, AR;Spreafico, A;Bedard, PL;Lheureux, S;Razak, A;Speers, V;Berman, HK;Aleshin, A;Haibe-Kains, B;Brooks, DG;McGaha, TL;Butler, MO;Bratman, SV;Ohashi, PS;Siu, LL;Pugh, TJ; | Pan-cancer analysis of longitudinal metastatic tumors reveals genomic alterations and immune landscape dynamics associated with pembrolizumab sensitivity | Nature communications | 34446728 | ary Fig. 9A. PD-L1 immunohistochemistry Immunohistochemical (IHC) staining for PD-L1 using the mouse monoclonal anti-PD-L1 antibody (clone 22C3 at 2 μg/mL, Merck, Palo Alto, CA) was performed by Qualtek Molecular Laboratories (Newtown, PA, USA). The | Full Paper |
2021 | Fowler, TW;Mitchell, TL;Janda, CY;Xie, L;Tu, S;Chen, H;Zhang, H;Ye, J;Ouyang, B;Yuan, TZ;Lee, SJ;Newman, M;Tripuraneni, N;Rego, ES;Mutha, D;Dilip, A;Vuppalapaty, M;Baribault, H;Yeh, WC;Li, Y; | Development of selective bispecific Wnt mimetics for bone loss and repair | Nature communications | 34059688 | The following method was used for human and mouse femur bone (Fig. 1d, e). Fresh human cadaveric femur tissue was purchased from vendor (Folio Conversant). Femur heads were shipped on wet ice within 24 h of death and tissue used immediately upon rece | Full Paper |
2021 | Butler, D;Mozsary, C;Meydan, C;Foox, J;Rosiene, J;Shaiber, A;Danko, D;Afshinnekoo, E;MacKay, M;Sedlazeck, FJ;Ivanov, NA;Sierra, M;Pohle, D;Zietz, M;Gisladottir, U;Ramlall, V;Sholle, ET;Schenck, EJ;Westover, CD;Hassan, C;Ryon, K;Young, B;Bhattacharya, C;Ng, DL;Granados, AC;Santos, YA;Servellita, V;Federman, S;Ruggiero, P;Fungtammasan, A;Chin, CS;Pearson, NM;Langhorst, BW;Tanner, NA;Kim, Y;Reeves, JW;Hether, TD;Warren, SE;Bailey, M;Gawrys, J;Meleshko, D;Xu, D;Couto-Rodriguez, M;Nagy-Szakal, D;Barrows, J;Wells, H;O'Hara, NB;Rosenfeld, JA;Chen, Y;Steel, PAD;Shemesh, AJ;Xiang, J;Thierry-Mieg, J;Thierry-Mieg, D;Iftner, A;Bezdan, D;Sanchez, E;Campion, TR;Sipley, J;Cong, L;Craney, A;Velu, P;Melnick, AM;Shapira, S;Hajirasouliha, I;Borczuk, A;Iftner, T;Salvatore, M;Loda, M;Westblade, LF;Cushing, M;Wu, S;Levy, S;Chiu, C;Schwartz, RE;Tatonetti, N;Rennert, H;Imielinski, M;Mason, CE; | Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactions | Nature communications | 33712587 | Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.++Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.++Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, U | Full Paper |
2021 | Li, J;Conrad, C;Mills, TW;Berg, NK;Kim, B;Ruan, W;Lee, JW;Zhang, X;Yuan, X;Eltzschig, HK; | PMN-derived netrin-1 attenuates cardiac ischemia-reperfusion injury via myeloid ADORA2B signaling | The Journal of experimental medicine | 33891683 | Materials and methods. MI patient and healthy donor samples. Serum/plasma samples of patients with MI or healthy donors were purchased from Discovery Life Sciences. Corresponding cTnI levels of patients with MI were documented in the patient sample d | Full Paper |
2021 | Dang, K;Castello, G;Clarke, SC;Li, Y;Balasubramani, A;Boudreau, A;Davison, L;Harris, KE;Pham, D;Sankaran, P;Ugamraj, HS;Deng, R;Kwek, S;Starzinski, A;Iyer, S;van Schooten, W;Schellenberger, U;Sun, W;Trinklein, ND;Buelow, R;Buelow, B;Fong, L;Dalvi, P; | Attenuating CD3 affinity in a PSMAxCD3 bispecific antibody enables killing of prostate tumor cells with reduced cytokine release | Journal for immunotherapy of cancer | 34088740 | Tumor cells were either isolated from fresh prostate tumor tissue procured from LF's lab at UCSF (San Francisco, California, USA) or received as frozen dissociated aliquots along with donor matched PBMCs from Discovery Life Sciences (Newtown, Pennsyl | Full Paper |
2021 | Nghiem, P;Bhatia, S;Lipson, EJ;Sharfman, WH;Kudchadkar, RR;Brohl, AS;Friedlander, PA;Daud, A;Kluger, HM;Reddy, SA;Boulmay, BC;Riker, A;Burgess, MA;Hanks, BA;Olencki, T;Kendra, K;Church, C;Akaike, T;Ramchurren, N;Shinohara, MM;Salim, B;Taube, JM;Jensen, E;Kalabis, M;Fling, SP;Homet Moreno, B;Sharon, E;Cheever, MA;Topalian, SL; | Three-year survival, correlates and salvage therapies in patients receiving first-line pembrolizumab for advanced Merkel cell carcinoma | Journal for immunotherapy of cancer | 33879601 | small T-antigen-specific serum antibodies21 or manifested large T-antigen expression in tumor biopsies via immunohistochemistry.22 PD-L1 staining (anti-PD-L1 clone 22C3, Merck & Co, Kenilworth, New Jersey, USA) was performed at QualTek Molecular | Full Paper |
2021 | Banchereau, R;Chitre, AS;Scherl, A;Wu, TD;Patil, NS;de Almeida, P;Kadel Iii, EE;Madireddi, S;Au-Yeung, A;Takahashi, C;Chen, YJ;Modrusan, Z;McBride, J;Nersesian, R;El-Gabry, EA;Robida, MD;Hung, JC;Kowanetz, M;Zou, W;McCleland, M;Caplazi, P;Eshgi, ST;Koeppen, H;Hegde, PS;Mellman, I;Mathews, WR;Powles, T;Mariathasan, S;Grogan, J;O'Gorman, WE; | Intratumoral CD103+ CD8+ T cells predict response to PD-L1 blockade | Journal for immunotherapy of cancer | 33827905 | containing regimen. Fresh tumor samples and matched adjacent non-cancerous tissues were procured from a commercial vendor (Discovery Life Sciences) as part of adult patients undergoing surgical resection. Online supplemental ;h locally advanced | Full Paper |
2021 | Badve, SS;Penault-Llorca, F;Reis-Filho, JS;Deurloo, R;Siziopikou, KP;D'Arrigo, C;Viale, G; | Determining PD-L1 Status in Patients with Triple-Negative Breast Cancer: Lessons Learned from IMpassion130 | Journal of the National Cancer Institute | 34286340 | CD has received consulting fees from Roche TD, Targos GmbH, Pfizer, Roche Pharma, LDPath Ltd, About Health Ltd, Uralensis INOV8 Ltd, and various NHS hospitals and has received nonfinan- cial support from UKNEQAS ICC&ISH and CADQAS cic | Full Paper |
2021 | Saura, C;Matito, J;Oliveira, M;Wildiers, H;Brufksy, AM;Waters, SH;Hurvitz, SA;Moy, B;Kim, SB;Gradishar, WJ;Queiroz, GS;Cronemberger, E;Wallweber, GJ;Bebchuk, J;Keyvanjah, K;Lalani, AS;Bryce, R;Vivancos, A;Eli, LD;Delaloge, S; | Biomarker Analysis of the Phase III NALA Study of Neratinib + Capecitabine versus Lapatinib + Capecitabine in Patients with Previously Treated Metastatic Breast Cancer | Clinical cancer research : an official journal of the American Association for Cancer Research | 34380637 | Yan (Puma Biotechnology). We thank Jeff Sperinde and Weidong Huang (Monogram Biosciences) for additional support for HERmark and p95 assay design and execution. We thank Sandra Bernard (Targos GMBH) for oversight of clinical sample management. We | Full Paper |
2021 | Pedersen, KS;Foster, NR;Overman, MJ;Boland, PM;Kim, SS;Arrambide, KA;Jaszewski, BL;Bekaii-Saab, T;Graham, RP;Welch, J;Wilson, RH;McWilliams, RR; | ZEBRA: A Multicenter Phase II Study of Pembrolizumab in Patients with Advanced Small-Bowel Adenocarcinoma | Clinical cancer research : an official journal of the American Association for Cancer Research | 33883178 | (clinicaltrials.gov). Biomarkers PD-L1 expression was centrally assessed (QualTek, Santa Barbara, CA) by immunohistochemical assays utilizing the Merck 22C3 antibody (Agilent/Dako). Samples required > 50 viable neoplastic cells. Modified Proportion S | Full Paper |
2021 | Larson, MH;Pan, W;Kim, HJ;Mauntz, RE;Stuart, SM;Pimentel, M;Zhou, Y;Knudsgaard, P;Demas, V;Aravanis, AM;Jamshidi, A; | A comprehensive characterization of the cell-free transcriptome reveals tissue- and subtype-specific biomarkers for cancer detection | Nature communications | 33883548 | We set out to validate the 23 cell-free mRNA biomarkers identified in our CCGA cohort in an orthogonal set of breast (n = 38) and lung (n = 18) cancer plasma samples obtained from a commercial vendor (Discovery Life Sciences) ;t to validate the | Full Paper |
2021 | Lorés-Motta, L;van Beek, AE;Willems, E;Zandstra, J;van Mierlo, G;Einhaus, A;Mary, JL;Stucki, C;Bakker, B;Hoyng, CB;Fauser, S;Clark, SJ;de Jonge, MI;Nogoceke, E;Koertvely, E;Jongerius, I;Kuijpers, TW;den Hollander, AI; | Common haplotypes at the CFH locus and low-frequency variants in CFHR2 and CFHR5 associate with systemic FHR concentrations and age-related macular degeneration | American journal of human genetics | 34260947 | associated with AMD (locus-wide conditioning).15 All the association analyses were performed with the “glm” R function.40 Immunocytochemistry of human retinas Human donor eyes were obtained from Discovery Life Sciences. Eyes were collected within 12 | Full Paper |
2021 | Egelston, C;Guo, W;Yost, S;Lee, JS;Rose, D;Avalos, C;Ye, J;Frankel, P;Schmolze, D;Waisman, J;Lee, P;Yuan, Y; | Pre-existing effector T-cell levels and augmented myeloid cell composition denote response to CDK4/6 inhibitor palbociclib and pembrolizumab in hormone receptor-positive metastatic breast cancer | Journal for immunotherapy of cancer | 33757987 | PD-L1 stain was performed using 22C3 antibody by Qualtek Molecular Laboratory | Full Paper |
2021 | Campbell, JR;McDonald, BR;Mesko, PB;Siemers, NO;Singh, PB;Selby, M;Sproul, TW;Korman, AJ;Vlach, LM;Houser, J;Sambanthamoorthy, S;Lu, K;Hatcher, SV;Lohre, J;Jain, R;Lan, RY; | Fc-optimized Anti-CCR8 Antibody Depletes Regulatory T Cells in Human Tumor Models | Cancer research | 33757978 | Study approval Human tumor, skin, spleen, patient blood, and healthy blood leukopack samples were collected through commercial providers (BioIVT, MT Group, Avaden, BioOptions, Discovery Life Sciences, AllCells) or the Cooperative Human Tissue Network | Full Paper |
2021 | Dieckmann, N;Schildhaus, HU;Bauer, S; | Tropomyosin receptor kinases in sarcomas - of joy and despair | Current opinion in oncology | 33989242 | Novartis, and Incyte; and received travel and accommodation funding from PharmaMar. H.U.S. is an employee of Targos Molecular pathology, Inc., received research funding from Novartis Oncology, has received honoria from MSD, BMS, Pfizer, Novartis Onco | Full Paper |
2021 | Yuan, Y;Lee, J;Yost, S;Frankel, P;Ruel, C;Egelston, C;Guo, W;Padam, S;Tang, A;Martinez, N;Schmolze, D;Presant, C;Ebrahimi, B;Yeon, C;Sedrak, M;Patel, N;Portnow, J;Lee, P;Mortimer, J; | Phase I/II trial of palbociclib, pembrolizumab and letrozole in patients with hormone receptor-positive metastatic breast cancer | European Journal of Cancer | 2.4. Tumour immune biomarkers Percentage of stromal TILs in tumour was evaluated using haematoxylin and eosin diagnostic sections per International TIL Work Group guideline [23]. PD-L1 was determined by immunohistochemistry (IHC) using the PD-L1 IHC | Full Paper | |
2021 | Liang, X;Wang, Y;Zhang, Y;Li, B;Radosevich, M; | Bacteriophage-host depth distribution patterns in soil are maintained after nutrient stimulation in vitro | The Science of the total environment | 33991924 | The extracted DNA samples were quantified using PicoGreen Assay Kit (Invitrogen, Carlsbad, CA) and were then sent to the HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA) for preparation of 16S rRNA gene amplicon library and high-throughp | Full Paper |
2021 | Quaas, A;Pamuk, A;Klein, S;Quantius, J;Rehkaemper, J;Barutcu, AG;Rueschoff, J;Zander, T;Gebauer, F;Hillmer, A;Buettner, R;Schroeder, W;Bruns, CJ;Löser, H;Schoemig-Markiefka, B;Alakus, H; | Sex-specific prognostic effect of CD66b-positive tumor-infiltrating neutrophils (TANs) in gastric and esophageal adenocarcinoma | Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association | 34009535 | Thomas Zander. Institute of Pathology, Nordhessen and Targos Molecular Pathology GmbH, Kassel, Germany. Josef Rueschoff. Authors: Alexander Quaas View author publications. You can also search for this author in PubMed Google Scholar | Full Paper |
2021 | Schoemig-Markiefka, B;Eschbach, J;Scheel, AH;Pamuk, A;Rueschoff, J;Zander, T;Buettner, R;Schroeder, W;Bruns, CJ;Loeser, H;Alakus, H;Quaas, A; | Optimized PD-L1 scoring of gastric cancer | Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association | 33954872 | Thomas Zander. Institute of Pathology Nordhessen, Kassel, Germany. Josef Rueschoff. Targos Molecular Pathology GmbH, Kassel, Germany. Josef Rueschoff & Reinhard Buettner. Authors: Birgid Schoemig-Markiefka View author publications | Full Paper |
2021 | Onogi, S;Lee, SH;Fruehauf, KR;Shea, KJ; | Abiotic Stimuli-Responsive Protein Affinity Reagent for IgG | Biomacromolecules | 34009976 | Transferrin (80 kDa) from human plasma was obtained from Calbiochem. Human serum was procured from Discovery Life Sciences, Inc. F(ab′) 2 (110 kDa) and Fc (50 kDa) fragments from human IgG were sourced from Jackson ImmunoResearch Laboratories, Inc | Full Paper |
2021 | Cao, XE;Kim, J;Mehta, S;Erickson, D; | Two-Color Duplex Platform for Point-of-Care Differential Detection of Malaria and Typhoid Fever | Analytical chemistry | 34469115 | samples in serum. 2.4. Validation with Human Clinical Samples. The clinical validation was conducted to evaluate the performance of the duplex platform with 25 human samples (Discovery Life Sciences). These included eight | Full Paper |
2021 | Narla, RK;Modi, H;Bauer, D;Abbasian, M;Leisten, J;Piccotti, JR;Kopytek, S;Eckelman, BP;Deveraux, Q;Timmer, J;Zhu, D;Wong, L;Escoubet, L;Raymon, HK;Hariharan, K; | Modulation of CD47-SIRPα innate immune checkpoint axis with Fc-function detuned anti-CD47 therapeutic antibody | Cancer immunology, immunotherapy : CII | 34247273 | lenalidomide [30]. Cell samples from AML patients were obtained from Conversant Bio (Huntsville) and are described in Supplemental Table 1. CC-90002 binding on cells and blockade of SIRPα to CD47 positive cells. Evaluation of | Full Paper |
2021 | Vos, H;Lambein, K;Richard, F;Mariën, B;Nevelsteen, I;Punie, K;Wildiers, H;Berben, L;Laenen, A;Floris, G;Desmedt, C;Smeets, A; | Comparison of the tumor immune microenvironment of primary hormone receptor-negative HER2-positive and triple negative breast cancer | NPJ breast cancer | 34556657 | PD-L1 (22C3 antibody, 2 μg/mL, Dako-Agilent) immunohistochemistry was performed by Qualtek Molecular Laboratories (Newtown PA, USA) as per agreement with Merck. QualTek provided a modified proportion score (MPS | Full Paper |
2021 | Pierson, SK;Shenoy, S;Oromendia, AB;Gorzewski, AM;Langan Pai, RA;Nabel, CS;Ruth, JR;Parente, SAT;Arenas, DJ;Guilfoyle, M;Reddy, M;Weinblatt, M;Shadick, N;Bower, M;Pria, AD;Masaki, Y;Katz, L;Mezey, J;Beineke, P;Lee, D;Tendler, C;Kambayashi, T;Fosså, A;van Rhee, F;Fajgenbaum, DC; | Discovery and validation of a novel subgroup and therapeutic target in idiopathic multicentric Castleman disease | Blood advances | 34438448 | this study. We wish to thank Daniel Martinez and the other members of the CHOP Pathology Core as well as Qualtek for their immunohistochemical analyses. We wish to thank Kevin Silk, Giovanni Insana, and James A. Lebovitz for their contributions to th | Full Paper |
2021 | Wang, A;Song, Z;Zheng, G;Nicolazzi, C;Fromm, JR;Shehu, E;Srinivasan, S;Chen, X;Zhu, C;Blondel, MC;Adrian, FJ; | Evaluation of Preclinical Activity of Isatuximab in Patients with Acute Lymphoblastic Leukemia | Molecular cancer therapeutics | 34376579 | centrifugation in line with the manufacturer's instructions. Patient ALL PBMCs were obtained from Conversant Biologics, Inc. Antibodies and reagents Isatuximab was generated in-house. Human immunoglobulin (Ig) G1, IgG4, ATRA | Full Paper |
2021 | Reardon, DA;Kim, TM;Frenel, JS;Simonelli, M;Lopez, J;Subramaniam, DS;Siu, LL;Wang, H;Krishnan, S;Stein, K;Massard, C; | Treatment with pembrolizumab in programmed death ligand 1-positive recurrent glioblastoma: Results from the multicohort phase 1 KEYNOTE-028 trial | Cancer | 33496357 | QualTek Molecular Laboratories evaluated archived or newly obtained formalin-fixed, paraffin-embedded tumor samples using the 22C3 PD-L1 antibody clone (Merck & Co., Inc., Kenilworth, NJ, USA) and a laboratory- developed prototype immunohistochemical | Full Paper |
2021 | Preillon, J;Cuende, J;Rabolli, V;Garnero, L;Mercier, M;Wald, N;Pappalardo, A;Denies, S;Jamart, D;Michaux, AC;Pirson, R;Pitard, V;Bagot, M;Prasad, S;Houthuys, E;Brouwer, M;Marillier, R;Lambolez, F;Marchante, JR;Nyawouame, F;Carter, MJ;Baron-Bodo, V;Marie-Cardine, A;Cragg, M;Déchanet-Merville, J;Driessens, G;Hoofd, C; | Restoration of T-cell Effector Function, Depletion of Tregs, and Direct Killing of Tumor Cells: The Multiple Mechanisms of Action of a-TIGIT Antagonist Antibodies | Molecular cancer therapeutics | 33277440 | Cryopreserved isolated PBMCs from healthy volunteers and patients with cancer were provided by ImmunXperts and ConversantBio, respectively. PBMCs were thawed, resuspended in RPMI medium supplemented with 10% FBS + 50 U/mL penicillin/streptomycin (Wes | Full Paper |
2021 | MacFarlane, AW;Yeung, HM;Alpaugh, RK;Dulaimi, E;Engstrom, PF;Dasari, A;Campbell, KS;Vijayvergia, N; | Impacts of pembrolizumab therapy on immune phenotype in patients with high-grade neuroendocrine neoplasms | Cancer immunology, immunotherapy : CII | 33398390 | PD-L1 immunohistochemistry (IHC) assay was performed at Qualtek Research Laboratories on formalin-fixed, paraffin-embedded tissue sections using anti-PD-L1 monoclonal antibody clone 22C3 (Merck Research Laboratories) | Full Paper |
2021 | Holder, C;Adams, A;Allison, C;Cote, O;Lippens, R;Blount, BC;Wang, L; | A Novel UHPLC-MS/MS Method for Measuring 8-iso-Prostaglandin F2α in Bronchoalveolar Lavage Fluid | Frontiers in chemistry | 34476231 | Non-EVALI BAL fluids used in this study were acquired from Discovery Life Sciences (Huntsville, AL, United States) | Full Paper |
2021 | Rodrigues, D;de Souza, T;Coyle, L;Di Piazza, M;Herpers, B;Ferreira, S;Zhang, M;Vappiani, J;Sévin, DC;Gabor, A;Lynch, A;Chung, SW;Saez-Rodriguez, J;Jennen, DGJ;Kleinjans, JCS;de Kok, TM; | New insights into the mechanisms underlying 5-fluorouracil-induced intestinal toxicity based on transcriptomic and metabolomic responses in human intestinal organoids | Archives of toxicology | 34151400 | 5-Fluorouracil (5-FU) is a widely used chemotherapeutical that induces acute toxicity in the small and large intestine of patients. Symptoms can be severe. ;- 5-Fluorouracil (5-FU) is a widely used chemotherapeutical that induces acute toxicity in | Full Paper |
2021 | Comunale, BA;Engineer, L;Jiang, Y;Andrews, JC;Liu, Q;Ji, L;Yurkovich, JT;Comunale, RA;Xie, Q; | Poliovirus Vaccination Induces a Humoral Immune Response That Cross Reacts With SARS-CoV-2 | Frontiers in medicine | 34414206 | ccination protocols, set by the Centers for Disease Control and Prevention (CDC). IRB approval for the collection of adult sera was obtained from BioMed IRB, and pediatric samples were purchased from Discovery Life Sciences (Huntsville, AL) in accord | Full Paper |
2021 | Haynes, WA;Kamath, K;Waitz, R;Daugherty, PS;Shon, JC; | Protein-Based Immunome Wide Association Studies (PIWAS) for the Discovery of Significant Disease-Associated Antigens | Frontiers in immunology | 33986742 | years, with a range of 22-72. Anti-Smith Cohort Samples from 34 subjects that tested positive for Anti-SM RNP (4) or Anti-Smith (30) antibodies by predicate ANA multiplex testing were obtained from Discovery Life Sciences. Subjects ranged in age fro | Full Paper |
2021 | Kerr, KM;Bibeau, F;Thunnissen, E;Botling, J;Ryška, A;Wolf, J;Öhrling, K;Burdon, P;Malapelle, U;Büttner, R; | The evolving landscape of biomarker testing for non-small cell lung cancer in Europe | Lung cancer (Amsterdam, Netherlands) | 33690091 | He is a co-owner and CSO of Targos Molecular Pathology, Inc., Germany | Full Paper |
2021 | Gentzel, RC;Toolan, D;Jinn, S;Schachter, JB;Ma, L;Kahle, PJ;Smith, SM;Marcus, JN; | Intracranial administration of alpha-synuclein fibrils in A30P-synuclein transgenic mice causes robust synucleinopathy and microglial induction | Neurobiology of aging | 34225000 | comparison. Neuropathologically confirmed human post-mortem PD brain tissue, acquired from Folio/Discovery Life Sciences (California, USA), or A30P HET brain tissues were serially extracted to obtain a sarkosyl insoluble fraction | Full Paper |
2021 | Malik-Chaudhry, HK;Prabhakar, K;Ugamraj, HS;Boudreau, AA;Buelow, B;Dang, K;Davison, LM;Harris, KE;Jorgensen, B;Ogana, H;Pham, D;Schellenberger, U;Van Schooten, W;Buelow, R;Iyer, S;Trinklein, ND;Rangaswamy, US; | TNB-486 induces potent tumor cell cytotoxicity coupled with low cytokine release in preclinical models of B-NHL | mAbs | 33818299 | ground value. The percent of specific lysis was calculated as described above. Ex vivo cytotoxicity assay The ability of TNB-486 to kill CD19+ tumor cells from CLL or DLBCL patient-derived samples (Discovery Life Sciences and IQ Biosciences) was det | Full Paper |
2021 | Yamada, M;Eguchi, A;Okuno, K;Sakaguchi, K;Yamaguchi, T; | Development of a highly sensitive chemiluminescent enzyme immunoassay for fragmented cytokeratin 18 using new antibodies | Scientific reports | 34521905 | All methods were carried out in accordance with relevant guidelines and regulations. Human samples. Serum from healthy individuals and NASH patients was purchased from Discovery Life Sciences (CA, USA) with approval and informed consent;defined below | Full Paper |
2021 | Awad, H;Tili, E;Nuovo, G;Kelani, H;Ramadan, ME;Williams, J;Binzel, K;Rajan, J;Mast, D;Efanov, AA;Rasul, KB;Moore, S;Basso, M;Mikhail, A;Eltobgy, M;Malbrue, RA;Bourekas, E;Oglesbee, M;Bergdall, V;Knopp, M;Michaille, JJ;El-Sayed, H; | Endovascular repair and open repair surgery of thoraco-abdominal aortic aneurysms cause drastically different types of spinal cord injury | Scientific reports | 33837260 | The remainder of the tissue was stored at − 80 °C for metabolome analyses. All sections were subsequently analyzed blindly. Immunohistochemistry (IHC). All sections were dealt with blindly using an automated Leica Bond Max platform at Phylogeny Inc. | Full Paper |
2021 | Mezache, L;Nuovo, G;Veeraraghavan, R; | A Multipronged Microscopy Approach Identifies Common Anti-Arrhythmic Strategy for Atrial Fibrillation and Myocardial Infarction | Microscopy and Microanalysis | 1 The Ohio State University, United States, 2 Discovery Life Sciences, United States Vascular leak and inflammation are associated with arrhythmic heart diseases such as atrial fibrillation (AF) and myocardial infarction (MI) | Full Paper | |
2021 | Ermann, J;Matmusaev, M;Haley, E;Braun, C;Jost, F;Mayer-Wrangowski, S;Hsiao, P;Ting, N;Li, L;Terenzio, D;Chime, J;Lukas, S;Patnaude, L;Panzenbeck, M;Csordas, D;Zheng, J;Mierz, D;Simpson, T;King, FJ;Klimowicz, A;Mbow, ML;Fine, JS;Miller, C;Fogal, S;Byrne, FR; | The potent and selective RIPK2 inhibitor BI 706039 improves intestinal inflammation in the TRUC mouse model of Inflammatory Bowel Disease | American journal of physiology. Gastrointestinal and liver physiology | 34494462 | Human IBD Intestinal biopsy Immuno-histo-chemical analysis 231 Tissue blocks from surgical resections for ulcerative colitis (n=6) and Crohn's Disease (n=5) were 232 acquired from Discovery Life Sciences. 4 µm sections were fluorescently stained usin | Full Paper |
2021 | Reisenauer, KN;Tao, Y;Das, P;Song, S;Svatek, H;Patel, SD;Mikhail, S;Ingros, A;Sheesley, P;Masi, M;Boari, A;Evidente, A;Kornienko, A;Romo, D;Taube, J; | Epithelial-mesenchymal transition sensitizes breast cancer cells to cell death via the fungus-derived sesterterpenoid ophiobolin A | Scientific reports | 34017048 | ke to acknowledge Dr. Michelle Nemec, Director of the Molecular Bioscience Center (Baylor University) for assistance with flow cytometry and Dr. Maria Boccia (Baylor University) and Dr. Gerard Nuovo (Discovery Life Sciences, Powell, OH, USA) for assi | Full Paper |
2021 | Caldwell, C;Rottman, JB;Paces, W;Bueche, E;Reitsma, S;Gibb, J;Adisetiyo, V;Haas, MS;Heath, H;Newman, W;Baum, J;Gianani, R;Kagey, MH; | Validation of a DKK1 RNAscope chromogenic in situ hybridization assay for gastric and gastroesophageal junction adenocarcinoma tumors | Scientific reports | 33972574 | chromogen kit (Biocare Medical). RNAscope. G/GEJ FFPE tumor resections were commercially acquired from Avaden Biosciences, Discovery Life Sciences or BioOptions following IRB compliance. RNAscope was performed ;emperature for 30 min, washed, and | Full Paper |
2021 | Wong, RL;Yu, EY; | Refining Immuno-Oncology Approaches in Metastatic Prostate Cancer: Transcending Current Limitations | Current treatment options in oncology | 33433743 | 028 phase 1b trial of pembrolizumab in PD-L1-positive advanced solid tumors included 23 patients with CRPC and PD-L1 expression ≥ 1% in tumor or stroma cells by a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek Molecular | Full Paper |
2021 | Meyer, L;Delgado-Cunningham, K;Lorig-Roach, N;Ford, J;DuBois, RM; | Human Astrovirus 1-8 Seroprevalence Evaluation in a United States Adult Population | Viruses | 34070419 | 2.7. Human Plasma Samples. Human plasma samples were obtained from Discovery Life Sciences Supplementary Materials. The following are available online at https://www.mdpi. com/article/10.3390/v13060979/s1, Table S1: Plasma Samples from Discovery Li | Full Paper |
2021 | Lopez-Anido, RN;Harrington, AM;Hamlin, HJ; | Coping with stress in a warming Gulf: the postlarval American lobster's cellular stress response under future warming scenarios | Cell stress & chaperones | 34115338 | The RNA samples from five replicate animals reared at each temperature were shipped to HudsonAlpha Discovery (formerly the Genomic Services Lab) at the HudsonAlpha Institute for Biotechnology (Huntsville, AL, USA) for library preparation and sequenci | Full Paper |
2021 | Magro, C;Crowson, A;Franks, L;Schaffer, P;Whelan, P;Nuovo, G; | The histologic and molecular correlates of COVID-19 vaccine-induced changes in the skin | Clinics in Dermatology | onald O. Perelman Department of Dermatology, New York University Grossman School of Medicine, New York, New York eDepartment of Pediatrics, Geffen School of Medicine at UCLA, Los Angeles, California fDiscovery Life Sciences, Powell, Ohio gThe Ohio St | Full Paper | |
2021 | Magro, C;Nuovo, G;Mulvey, J;Laurence, J;Harp, J;Crowson, A; | The skin as a critical window in unveiling the pathophysiologic principles of COVID-19 | Clinics in Dermatology | Joanna MD e Crowson A. Neil MD f aDepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York bThe Ohio State University Comprehensive Cancer Center, Columbus Ohio and Discovery Life Sciences, Powell, Ohio cDepartment | Full Paper | |
2021 | Xia, B;Blount, BC;Wang, L; | Sensitive Quantification of Nicotine in Bronchoalveolar Lavage Fluid by Acetone Precipitation Combined With Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry | ACS omega | 34124421 | We directly injected 1 μL of the residual supernatant onto the LC-MS/MS. BAL fluid pools were created for method validation experiments of the method from 12 anonymous BAL fluids purchased commercially from Discovery Life Sciences (Huntsville, AL) | Full Paper |
2021 | Cirillo, PDR;Margiotti, K;Fabiani, M;Barros-Filho, MC;Sparacino, D;Cima, A;Longo, SA;Cupellaro, M;Mesoraca, A;Giorlandino, C; | Multi-analytical test based on serum miRNAs and proteins quantification for ovarian cancer early detection | PloS one | 34352040 | All serum samples from ovarian cancer patients were obtained through MTA (Material Transfer Agreement) signed consent form from the following centers: Discovery Life Sciences (Huntsville, USA), BioIVT (London, UK), Victorian Cancer Bank (Melbourne, A | Full Paper |
2021 | Walsh, CS;Kamrava, M;Rogatko, A;Kim, S;Li, A;Cass, I;Karlan, B;Rimel, BJ; | Phase II trial of cisplatin, gemcitabine and pembrolizumab for platinum-resistant ovarian cancer | PloS one | 34081738 | that had membrane staining at low (1+) intensities or greater. An H-score was calculated ranging from 0–300, reflecting the percentage of cells staining at each of the following intensities: negative (1), low (1+), moderate (2+), high (3+). IHC assay | Full Paper |
2021 | Bernstein, DE;Trinh, HN;Schiff, ER;Smith, CI;Mospan, AR;Zink, RC;Fried, MW;Lok, AS; | Safety and Effectiveness of Tenofovir Alafenamide in Usual Clinical Practice Confirms Results of Clinical Trials: TARGET-HBV | Digestive diseases and sciences | 34059991 | ERS: Grant support: Eiger, Galmed, Zydus, Celgene, Beckman, Biokit, Bristol Myers Squibb, Conatus, Celgene, Discovery Life Sciences, Genfit, Gilead, Intercept, Novartis, Novo Nordisk, Orasure Technologies, Ortho Diagnostics, Pfizer, Prometheus Lab, R | Full Paper |
2021 | Varlet, P;Bouffet, E;Casanova, M;Giangaspero, F;Antonelli, M;Hargrave, D;Ladenstein, R;Pearson, A;Hawkins, C;König, FB;Rüschoff, J;Schmauch, C;Bühnemann, C;Garin-Chesa, P;Schweifer, N;Uttenreuther-Fischer, M;Gibson, N;Ittrich, C;Krämer, N;Solca, F;Stolze, B;Geoerger, B; | Comprehensive analysis of the ErbB receptor family in pediatric nervous system tumors and rhabdomyosarcoma | Pediatric blood & cancer | 34546642 | Institute, Medical University, Vienna, Austria 8 Paediatric Drug Development, Children and Young People's Unit, Royal Marsden Hospital, London, UK 9 Division of Clinical Studies, Institute of Cancer Research, London, UK 10 Targos Molecular Pathology | Full Paper |
2021 | Bio, S;Nunes, B; | Twists and turns of an oyster's life: effects of different depuration periods on physiological biochemical functions of oysters | Environmental science and pollution research international | 33559825 | Aquaculture activities are often established in the vicinity of highly populated, potentially contaminated areas. Animals cultured at such locations, namely bivalves, are frequently used as test organisms in ecotoxicological testing. In this case, a | Full Paper |
2021 | Chen, YC;Figliozzi, RW;Hsia, SV; | Pilot Analyses of Interferon Subtype Expression Profiles in Patients with Herpes Zoster or Postherpetic Neuralgia | Viral immunology | 33857386 | A variety of blood samples containing ICD10 codes of HZ or PHN (Table 1) were purchased from Discovery Life Sciences (800 Hudson Way, Suite 1700, Huntsville, AL 35806) The sera of healthy controls were purchased from Discovery Life Sciences with an | Full Paper |
2021 | Kam, K;Kumar, V;Lamport, Z;Kymissis, I; | A laboratory course on information display technologies for remote learning | Journal of the Society for Information Display | Survey response percentage, 55.55%. TABLE 2. Listed components and suppliers Component, Brand. AC/DC wall mount adapter 12 V 12 W, Qualtek. LED matrix 5 × 7, Kingbright. Fan axial 40 × 10mm 12VDC wire, CUI Devices. Switch push, C&K | Full Paper | |
2021 | Rüschoff, J;Baretton, G;Bläker, H;Dietmaier, W;Dietel, M;Hartmann, A;Horn, LC;Jöhrens, K;Kirchner, T;Knüchel, R;Mayr, D;Merkelbach-Bruse, S;Schildhaus, HU;Schirmacher, P;Tiemann, M;Tiemann, K;Weichert, W;Büttner, R; | MSI testing : What's new? What should be considered? | Der Pathologe | 34477921 | Institute of Pathology, Nordhessen und Targos Molecular Pathology GmbH, Germaniastr. 7, 34119, Kassel, Germany. josef.rueschoff@targos-gmbh.de.++Institute of Pathology, University Hospital Carl Gustav Carus, Fetscherstr. 74, 01307, Dresden, Germany.+ | Full Paper |
2021 | Rüschoff, J;Baretton, G;Bläker, H;Dietmaier, W;Dietel, M;Hartmann, A;Horn, LC;Jöhrens, K;Kirchner, T;Knüchel, R;Mayr, D;Merkelbach-Bruse, S;Schildhaus, HU;Schirmacher, P;Tiemann, M;Tiemann, K;Weichert, W;Büttner, R; | [MSI testing : What is new? What should be considered? German version] | Der Pathologe | 34043067 | What should be considered? German version]. Josef Ruschoff Institut fur Pathologie Nordhessen, TARGOS Molecular Pathology GmbH, Germaniastr Affiliations. 1 author 1. Institut fur Pathologie Nordhessen, TARGOS Molecular Pathology GmbH, Germaniastr | Full Paper |
2021 | Schildhaus, HU;Weichert, W; | [Predictive diagnostics for checkpoint inhibitors] | Der Pathologe | 33956171 | H.-U. Schildhaus ist Mitarbeiter der Targos Molecular Pathology GmbH und gibt Honorare und Advisory Boards von MSD, BMS, AstraZeneca, Pfizer, | Full Paper |
2021 | Cromer, MK;Camarena, J;Martin, RM;Lesch, BJ;Vakulskas, CA;Bode, NM;Kurgan, G;Collingwood, MA;Rettig, GR;Behlke, MA;Lemgart, VT;Zhang, Y;Goyal, A;Zhao, F;Ponce, E;Srifa, W;Bak, RO;Uchida, N;Majeti, R;Sheehan, VA;Tisdale, JF;Dever, DP;Porteus, MH; | Gene replacement of α-globin with β-globin restores hemoglobin balance in β-thalassemia-derived hematopoietic stem and progenitor cells | Nature medicine | 33737751 | Human CD34+ HSPCs were cultured as previously described18,24,33,36,46,47. CD34+ HSPCs were sourced from fresh cord blood (generously provided by the Binns Family program for Cord Blood Research); from frozen cord blood and Plerixafor- and/or G-CSF-mo | Full Paper |
2021 | Gaidt, MM;Morrow, A;Fairgrieve, MR;Karr, JP;Yosef, N;Vance, RE; | Self-guarding of MORC3 enables virulence factor-triggered immunity | Nature | 34759314 | (PBMCs) from de-identified donors were obtained from AllCells (Alameda) under donor-informed consent and Alpha IRB approval obtained by AllCells (7000-SOP-045) for the study ' Save | Full Paper |
2021 | Mimitou, EP;Lareau, CA;Chen, KY;Zorzetto-Fernandes, AL;Hao, Y;Takeshima, Y;Luo, W;Huang, TS;Yeung, BZ;Papalexi, E;Thakore, PI;Kibayashi, T;Wing, JB;Hata, M;Satija, R;Nazor, KL;Sakaguchi, S;Ludwig, LS;Sankaran, VG;Regev, A;Smibert, P; | Scalable, multimodal profiling of chromatin accessibility, gene expression and protein levels in single cells | Nature biotechnology | 34083792 | Cryopreserved healthy donor peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BM) were obtained from AllCells (USA) or Cellular Technology Limited (CTL) and processed immediately after thawing. NIH-3T3 and HEK293FT cells were maintain | Full Paper |
2021 | Lareau, CA;Ludwig, LS;Muus, C;Gohil, SH;Zhao, T;Chiang, Z;Pelka, K;Verboon, JM;Luo, W;Christian, E;Rosebrock, D;Getz, G;Boland, GM;Chen, F;Buenrostro, JD;Hacohen, N;Wu, CJ;Aryee, MJ;Regev, A;Sankaran, VG; | Massively parallel single-cell mitochondrial DNA genotyping and chromatin profiling | Nature biotechnology | 32788668 | Cryopreserved PBMCs from patients with CLL consented on institutional review board approved protocols were obtained from AllCells (Patient 1) or from Adrian Wiestner at the Save | Full Paper |
2021 | Hao, Y;Hao, S;Andersen-Nissen, E;Mauck, WM;Zheng, S;Butler, A;Lee, MJ;Wilk, AJ;Darby, C;Zager, M;Hoffman, P;Stoeckius, M;Papalexi, E;Mimitou, EP;Jain, J;Srivastava, A;Stuart, T;Fleming, LM;Yeung, B;Rogers, AJ;McElrath, JM;Blish, CA;Gottardo, R;Smibert, P;Satija, R; | Integrated analysis of multimodal single-cell data | Cell | 34062119 | stom made clone UCHL1 ------------------------- Biological samples ------------------------- Human PBMC Cape Town HVTN Immunology Lab, South Africa https://www.chil.org.za/ Human PBMC AllCells Lot 3032552 ------------------------- | Full Paper |
2021 | Okuchi, Y;Reeves, J;Ng, SS;Doro, DH;Junyent, S;Liu, KJ;El Haj, AJ;Habib, SJ; | Wnt-modified materials mediate asymmetric stem cell division to direct human osteogenic tissue formation for bone repair | Nature materials | 32958876 | hSSCs were isolated from a commercially available bone marrow aspirate (AllCells). The bone marrow aspirate was plated onto culture flasks precoated with 10 ng ml−1 fibronectin (Sigma-Aldrich, 356000) and the cells were allowed to attach. Surface mar | Full Paper |
2021 | Wu, SZ;Al-Eryani, G;Roden, DL;Junankar, S;Harvey, K;Andersson, A;Thennavan, A;Wang, C;Torpy, JR;Bartonicek, N;Wang, T;Larsson, L;Kaczorowski, D;Weisenfeld, NI;Uytingco, CR;Chew, JG;Bent, ZW;Chan, CL;Gnanasambandapillai, V;Dutertre, CA;Gluch, L;Hui, MN;Beith, J;Parker, A;Robbins, E;Segara, D;Cooper, C;Mak, C;Chan, B;Warrier, S;Ginhoux, F;Millar, E;Powell, JE;Williams, SR;Liu, XS;O'Toole, S;Lim, E;Lundeberg, J;Perou, CM;Swarbrick, A; | A single-cell and spatially resolved atlas of human breast cancers | Nature genetics | 34493872 | Percentage of neoplastic cells in each tumor that are classified as each of the SCSubtypes. Tumor samples are grouped according to their Allcells-Pseudobulk classifications. NL, normal-like. c, Representative images of CK5 (top) and ER (bottom) IHC f | Full Paper |
2021 | Ge, Y;Tian, T;Huang, S;Wan, F;Li, J;Li, S;Wang, X;Yang, H;Hong, L;Wu, N;Yuan, E;Luo, Y;Cheng, L;Hu, C;Lei, Y;Shu, H;Feng, X;Jiang, Z;Wu, Y;Chi, Y;Guo, X;Cui, L;Xiao, L;Li, Z;Yang, C;Miao, Z;Chen, L;Li, H;Zeng, H;Zhao, D;Zhu, F;Shen, X;Zeng, J; | An integrative drug repositioning framework discovered a potential therapeutic agent targeting COVID-19 | Signal transduction and targeted therapy | 33895786 | e performed at a flow rate of 30 μL/min. Data processing and analyses were performed using BIAevaluation 1.1 software. LPS-induced cytokine production in PBMCs Peripheral blood mononuclear cells (Allcells) were cultured at 37 °C at concentration 5% | Full Paper |
2021 | Miller, IC;Zamat, A;Sun, LK;Phuengkham, H;Harris, AM;Gamboa, L;Yang, J;Murad, JP;Priceman, SJ;Kwong, GA; | Enhanced intratumoural activity of CAR T cells engineered to produce immunomodulators under photothermal control | Nature biomedical engineering | 34385695 | Primary human CD3+ cells were obtained from an anonymous donor blood after apheresis (AllCells) and were cryopreserved in 90% FBS and 10% dimethylsulfoxide until subsequent Save | Full Paper |
2021 | Frede, J;Anand, P;Sotudeh, N;Pinto, RA;Nair, MS;Stuart, H;Yee, AJ;Vijaykumar, T;Waldschmidt, JM;Potdar, S;Kloeber, JA;Kokkalis, A;Dimitrova, V;Mann, M;Laubach, JP;Richardson, PG;Anderson, KC;Raje, NS;Knoechel, B;Lohr, JG; | Dynamic transcriptional reprogramming leads to immunotherapeutic vulnerabilities in myeloma | Nature cell biology | 34675390 | registration was 68.5 years (range 45-80). Myeloma cells were enriched using the EasySep Human CD138 positive selection kit (STEMCELL Technologies). Bone marrow from normal donors was obtained from AllCells. Single cell sorting Bone marrow cells wer | Full Paper |
2021 | Duault, C;Kumar, A;Taghi Khani, A;Lee, SJ;Yang, L; | Activated natural killer cells predict poor clinical prognosis in high-risk B-and T-cell acute lymphoblastic leukemia | blood | 36107453 | processed from patients with B/T-ALL and healthy donors according to Institutional Review Board policies. Healthy BMMCs were purchased from AllCells (Alameda, CA). Healthy PBMCs were isolated from buffy coats procured from Stanford Blood Center, City | Full Paper |
2021 | Duault, C;Kumar, A;Taghi Khani, A;Lee, SJ;Yang, L;Huang, M;Hurtz, C;Manning, B;Ghoda, L;McDonald, T;Lacayo, NJ;Sakamoto, KM;Carroll, M;Tasian, SK;Marcucci, G;Yu, J;Caligiuri, MA;Maecker, HT;Swaminathan, S; | Activated natural killer cells predict poor clinical prognosis in high-risk B- and T-cell acute lymphoblastic leukemia | Blood | 34077953 | ripheral blood mononuclear cells (PBMCs) were collected and processed from patients with B/T-ALL and healthy donors according to Institutional Review Board policies. Healthy BMMCs were purchased from AllCells (Alameda, CA). Healthy PBMCs were isolate | Full Paper |
2021 | Alavi, A;Krishnamurti, L;Abedi, M;Galeon, I;Reiner, D;Smith, S;Wang, L;Ramezi, A;Rendo, P;Walters, M; | Preliminary Safety and Efficacy Results from Precizn-1: An Ongoing Phase 1/2 Study on Zinc Finger Nuclease-Modified Autologous CD34+ HSPCs for Sickle Cell Disease (SCD) | Blood | Introduction Sickle cell disease (SCD) is caused by pathologic variants in both alleles of the β-globin gene, affecting ~100,000 patients in the US (Strouse. Handb Clin Neurol 2016;138:311-24). Elevated fetal hemoglobin (HbF) levels am | Full Paper | |
2021 | Kwiatkowski, J;Locatelli, F;Walters, M;Porter, J;Hongeng, S;Yannaki, E;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Guo, R;Colvin, R;Gruppioni, K;Thompson, A; | Improvement in Health-Related Quality of Life Following Treatment with Betibeglogene Autotemcel in Patients with Transfusion-Dependent β-Thalassemia Enrolled in Phase 3 Studies | Blood | Introduction: Patients with transfusion-dependent β-thalassemia (TDT) have increased morbidity and mortality and reduced health-related quality of life (HRQoL) compared with the general population owing to the mental, physical, and time demands of ch | Full Paper | |
2021 | Hall, A;Winestone, L;Sullivan, E;Wu, Q;Lamble, A;Walters, M;Aguayo-Hiraldo, P;Baez Conde, L;Coker, T;Dave, H;Dornsife, D;Keating, A;Merino, D;Ramsey, B;Park, J;Agrawal, A; | Access to CAR-T Cell Therapy in Underrepresented Populations: A Multicenter Cohort Study of Pediatric and Young Adult ALL Patients | Blood | Introduction: Social determinants of health such as race, ethnicity, and socioeconomic status (SES) are associated with inferior outcomes in patients with B-cell acute lymphoblastic leukemia (B-ALL). Latinx patients have worse event-free survival and | Full Paper | |
2021 | Thompson, A;Locatelli, F;Yannaki, E;Walters, M;Porter, J;Hongeng, S;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Cavazzana, M;Rasko, J;Du, L;Colvin, R;Kwiatkowski, J; | Restoring Iron Homeostasis in Pts Who Achieved Transfusion Independence after Treatment with Betibeglogene Autotemcel Gene Therapy: Results from up to 7 Years of Follow-up | Blood | Introduction: Transfusion-dependent β-thalassemia (TDT) is a severe genetic disease requiring lifelong, regular packed red blood cell (pRBC) transfusions and iron chelation treatment. Betibeglogene autotemcel (beti-cel) is a one-time ex vivo gene the | Full Paper | |
2021 | Walters, M;Smith, A;Schiller, G;Esrick, E;Williams, D;Gogoleva, T;Rouy, D;Cockroft, B;Vercellotti, G; | Updated Results of a Phase 1/2 Clinical Study of Zinc Finger Nuclease-Mediated Editing of BCL11A in Autologous Hematopoietic Stem Cells for Transfusion-Dependent Beta Thalassemia | Blood | Introduction: Persistently elevated fetal hemoglobin (HbF) can ameliorate transfusion-dependent beta thalassemia (TDT) (Musallam et al ., 2012). BCL11A is a repressor of γ-globin expression and HbF production in adult erythrocytes. Down-regulation of | Full Paper | |
2021 | Tisdale, J;Thompson, A;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Schmidt, M;Pierciey, F;Whitney, D;Rogers, C;Nnamani, M;Foos, M;Miller, A;Zhang, X;Lynch, J;Walters, M;Kanter, J;Bonner, M; | Polyclonality Strongly Correlates with Biological Outcomes and Is Significantly Increased Following Improvements to the Phase 1/2 HGB-206 Protocol and Manufacturing of LentiGlobin for Sickle Cell Disease (SCD; bb1111) Gene Therapy (GT) | Blood | Background: The ongoing Phase 1/2 HGB-206 study (NCT02140554) of LentiGlobin for SCD (bb1111) GT uses a modified human β-globin gene that expresses an anti-sickling hemoglobin (HbA T87Q). The relationships between biological outcomes, clinical outcom | Full Paper | |
2021 | Walters, M;Tisdale, J;Mapara, M;Krishnamurti, L;Kwiatkowski, J;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Jaroscak, J;Garbinsky, D;Chirila, C;Gallagher, M;Zhang, X;Ho, P;Thompson, A;Kanter, J; | Sustained Improvements in Patient-Reported Quality of Life up to 24 Months Post-Treatment with LentiGlobin for Sickle Cell Disease (bb1111) Gene Therapy | Blood | Background: Sickle cell disease (SCD) is characterized by painful vaso-occlusive events (VOEs), progressive vasculopathy, hemolytic anemia, and organ damage resulting in frequent hospitalizations and decreased quality of life (QoL). The ongoing phase | Full Paper | |
2021 | Kruta, M;Sunshine, MJ;Chua, BA;Fu, Y;Chawla, A;Dillingham, CH;Hidalgo San Jose, L;De Jong, B;Zhou, FJ;Signer, RAJ; | Hsf1 promotes hematopoietic stem cell fitness and proteostasis in response to ex vivo culture stress and aging | Cell stem cell | 34388375 | Human cord blood derived CD34 + HSPCs were purchased from AllCells. Cell number and viability were assessed with a hemocytometer based on trypan blue exclusion. Save | Full Paper |
2021 | Turchiano, G;Andrieux, G;Klermund, J;Blattner, G;Pennucci, V;El Gaz, M;Monaco, G;Poddar, S;Mussolino, C;Cornu, TI;Boerries, M;Cathomen, T; | Quantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-Seq | Cell stem cell | 33626327 | Experimental models: cell lines human CD34+ HSPCs AllCells Cat.# CB008F human CD34+ HSPCs StemCell-Technologies Cat.# 70008.3 K562 N/A RRID:CVCL_0004; authenticated by STR profiling | Full Paper |
2021 | Mondala, PK;Vora, AA;Zhou, T;Lazzari, E;Ladel, L;Luo, X;Kim, Y;Costello, C;MacLeod, AR;Jamieson, CHM;Crews, LA; | Selective antisense oligonucleotide inhibition of human IRF4 prevents malignant myeloma regeneration via cell cycle disruption | Cell stem cell | 33476575 | In multiple myeloma, inflammatory and anti-viral pathways promote disease progression and cancer stem cell generation. Using diverse pre-clinical models, we investigated the role of Save | Full Paper |
2021 | Su, M;Lin, Y;Cui, C;Tian, X;Lai, L; | ERMAP is a B7 family-related molecule that negatively regulates T cell and macrophage responses | Cellular & molecular immunology | 32620788 | T cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan-T labeling system were purchased from ALLCELLS, LLC (Alameda, CA). Murine CD3+ T cells were purified from C57BL/6 mice by an immunomagnetic system (Milt | Full Paper |
2021 | Seo, H;Masuda, H;Asagoshi, K;Uchiki, T;Kawata, S;Sasaki, G;Yabuki, T;Miyai, S;Takahashi, N;Hashimoto, SI;Sawada, A;Takaiwa, A;Koyama, C;Tamai, K;Kurosawa, K;Lin, KY;Ohta, K;Nakazaki, Y; | Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell line | Cellular & molecular immunology | 32457406 | The germline-derived divergent hV H harboring CDR3 was prepared from normal human peripheral blood CD19 + B-cell cDNA (ALLCELLS) using degenerate primers that annealed to Save | Full Paper |
2021 | Sadras, T;Martin, M;Kume, K;Robinson, ME;Saravanakumar, S;Lenz, G;Chen, Z;Song, JY;Siddiqi, T;Oksa, L;Knapp, AM;Cutler, J;Cosgun, KN;Klemm, L;Ecker, V;Winchester, J;Ghergus, D;Soulas-Sprauel, P;Kiefer, F;Heisterkamp, N;Pandey, A;Ngo, V;Wang, L;Jumaa, H;Buchner, M;Ruland, J;Chan, WC;Meffre, E;Martin, T;Müschen, M; | Developmental partitioning of SYK and ZAP70 prevents autoimmunity and cancer | Molecular cell | 33878293 | Cord blood derived human CD34 + cells (ALLCELLS) were thawed and were lentivirally transduced with pCL6-ZAP70-GFP or an empty vector control. To obtain optimal transduction, Save | Full Paper |
2021 | Lattanzi, A;Camarena, J;Lahiri, P;Segal, H;Srifa, W;Vakulskas, CA;Frock, RL;Kenrick, J;Lee, C;Talbott, N;Skowronski, J;Cromer, MK;Charlesworth, CT;Bak, RO;Mantri, S;Bao, G;DiGiusto, D;Tisdale, J;Wright, JF;Bhatia, N;Roncarolo, MG;Dever, DP;Porteus, MH; | Development of β-globin gene correction in human hematopoietic stem cells as a potential durable treatment for sickle cell disease | Science translational medicine | 34135108 | Human CD34+ HSPCs were cultured in small-scale conditions as previously described (67, 20, 25, 68, 69). Frozen purified CD34+ HSPCs were sourced from plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA and STEMCELL Technol | Full Paper |
2021 | Jain, MS;Polanski, K;Conde, CD;Chen, X;Park, J;Mamanova, L;Knights, A;Botting, RA;Stephenson, E;Haniffa, M;Lamacraft, A;Efremova, M;Teichmann, SA; | MultiMAP: dimensionality reduction and integration of multimodal data | Genome biology | 34930412 | sed to identify genes whose expression changes significantly along the trajectory. Acquisition and processing of human PBMCs PBMCs from two donors were acquired from a LeukoLab (Clinical division of AllCells). Frozen PBMC samples were thawed quickly | Full Paper |
2021 | Thongon, N;Ma, F;Santoni, A;Marchesini, M;Fiorini, E;Rose, A;Adema, V;Ganan-Gomez, I;Groarke, EM;Gutierrez-Rodrigues, F;Chen, S;Lockyer, P;Schneider, S;Bueso-Ramos, C;Montalban-Bravo, G;Class, CA;Soltysiak, KA;Pellegrini, M;Sahin, E;Bertuch, AA;DiNardo, CD;Garcia-Manero, G;Young, NS;Dwyer, K;Colla, S; | Hematopoiesis under telomere attrition at the single-cell resolution | Nature communications | 34824242 | BM samples from HDs were obtained from AllCells. Written informed consent was obtained from all donors. The clinical characteristics and the PB counts of the individuals with Save | Full Paper |
2021 | Qiao, Y;Qiu, Y;Ding, J;Luo, N;Wang, H;Ling, X;Sun, J;Wu, Z;Wang, Y;Liu, Y;Guo, F;Sun, T;Shen, W;Zhang, M;Wu, D;Chen, B;Xu, W;Wang, X; | Cancer immune therapy with PD-1-dependent CD137 co-stimulation provides localized tumour killing without systemic toxicity | Nature communications | 34737267 | Human PBMCs were purchased from Allcells and the informed consent was obtained from all donors. Human PBMCs were cultured in RPMI 1640 medium supplemented with 10% FBS Save | Full Paper |
2021 | Duren, Z;Lu, WS;Arthur, JG;Shah, P;Xin, J;Meschi, F;Li, ML;Nemec, CM;Yin, Y;Wong, WH; | Sc-compReg enables the comparison of gene regulatory networks between conditions using single-cell data | Nature communications | 34362918 | itial analysis has considered the HiChIP data. We will extend our sc-compReg software to enable this type of analysis. Methods Single-cell ATAC Cryopreserved BMMCs from two donors were obtained from AllCells. One donor was a 93-year-old non-Hispanic | Full Paper |
2021 | Amit, I;Iancu, O;Levy-Jurgenson, A;Kurgan, G;McNeill, MS;Rettig, GR;Allen, D;Breier, D;Ben Haim, N;Wang, Y;Anavy, L;Hendel, A;Yakhini, Z; | CRISPECTOR provides accurate estimation of genome editing translocation and off-target activity from comparative NGS data | Nature communications | 34031394 | ubation at room temperature before the electroporation. Genome editing in mobilized CD34+ HSPCs and in HEK293/HEK293-Cas9 cell lines Human CD34+ HSPCs, derived from mobilized peripheral blood (MPB) (AllCells, Alameda, CA), were thawed and cultured f | Full Paper |
2021 | Lal, A;Chiang, ZD;Yakovenko, N;Duarte, FM;Israeli, J;Buenrostro, JD; | Deep learning-based enhancement of epigenomics data with AtacWorks | Nature communications | 33686069 | 0 or 1 representing whether that position was part of a CTCF binding motif on the reverse strand. Generation of dscATAC-seq data for FACS-isolated HSCs Cryopreserved human BMMCs were purchased from Allcells (catalog number BM, CR, MNC, 10 M). Cells | Full Paper |
2021 | Sharma, R;Dever, DP;Lee, CM;Azizi, A;Pan, Y;Camarena, J;Köhnke, T;Bao, G;Porteus, MH;Majeti, R; | The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells | Nature communications | 33473139 | For in vivo studies presented, cord blood-derived CD34 + cells were purchased from AllCells or STEMCELL Technologies and were thawed according to the manufacturer's Save | Full Paper |
2021 | Yang, KD;Belyaeva, A;Venkatachalapathy, S;Damodaran, K;Katcoff, A;Radhakrishnan, A;Shivashankar, GV;Uhler, C; | Multi-domain translation between single-cell imaging and sequencing data using autoencoders | Nature communications | 33397893 | nly limited functional data is available by acquiring chromatin imaging data. Methods Cell culture and immunostaning CD4+/CD45RA+ naive helper T-cells from human peripheral blood were purchased from AllCells. These cells were revived and cultured in | Full Paper |
2021 | Gnanapragasam, M;Planutis, A;Glassberg, J;Bieker, J; | Identification of a genomic DNA sequence that quantitatively modulates KLF1 expression in differentiating human hematopoietic cells | bioRxiv | CD34+ cells were purchased from AllCells or obtained from the Yale Cooperative Center of Excellence in Hematology. These were differentiated under three-phase protocols, initially based on (Antoniani et al., 2018; Yien and Bieker, 2012) but then late | Full Paper | |
2021 | Shy, B;Vykunta, V;Ha, A;Roth, T;Talbot, A;Nguyen, D;Chen, Y;Blaeschke, F;Vedova, S;Mamedov, M;Chung, J;Li, H;Wolf, J;Martin, T;Ye, L;Eyquem, J;Esensten, J;Marson, A; | Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturing | bioRxiv | Primary adult blood cells were obtained from anonymous healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc. or Allcells Inc, or as a Trima residual from Vitalant. If needed, peripheral blood mononuclear cells were i | Full Paper | |
2021 | Amin, OE;Colbeck, EJ;Daffis, S;Khan, S;Ramakrishnan, D;Pattabiraman, D;Chu, R;Micolochick Steuer, H;Lehar, S;Peiser, L;Palazzo, A;Frey, C;Davies, J;Javanbakht, H;Rosenberg, WMC;Fletcher, SP;Maini, MK;Pallett, LJ; | Therapeutic Potential of TLR8 Agonist GS-9688 (Selgantolimod) in Chronic Hepatitis B: Remodeling of Antiviral and Regulatory Mediators | Hepatology (Baltimore, Md.) | 33368377 | In select experiments, whole blood was obtained from AllCells (Alameda, CA) and the MT Group (Van Nuys, CA). In this instance, consent was obtained from the donor or donor's legal Save | Full Paper |
2021 | Shukla, S;Ying, W;Gray, F;Yao, Y;Simes, ML;Zhao, Q;Miao, H;Cho, HJ;González-Alonso, P;Winkler, A;Lund, G;Purohit, T;Kim, E;Zhang, X;Ray, JM;He, S;Nikolaidis, C;Ndoj, J;Wang, J;Jaremko, Ł;Jaremko, M;Ryan, RJH;Guzman, ML;Grembecka, J;Cierpicki, T; | Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domain | Nature chemical biology | 34155404 | CD34-positive bone marrow blood cells were obtained from Allcells (ABM021F) and stored in liquid nitrogen until use. For treatment experiments that lasted more than 4 d, media were Save | Full Paper |
2021 | Jang, S;Song, J;Kim, N;Bak, J;Jung, K;Park, YW;Park, BC;Kim, HM; | Development of an antibody-like T-cell engager based on VH-VL heterodimer formation and its application in cancer therapy | Biomaterials | 33774526 | All healthy donor PBMCs and CD8 + T cells used in this study were purchased from AllCells (#PB004F and #PB009-3 F), Zenbio (#SER-PBMC-200-F), and Lonza (#3W-270). CD3 + T Save | Full Paper |
2021 | Davis-Marcisak, EF;Fitzgerald, AA;Kessler, MD;Danilova, L;Jaffee, EM;Zaidi, N;Weiner, LM;Fertig, EJ; | Transfer learning between preclinical models and human tumors identifies a conserved NK cell activation signature in anti-CTLA-4 responsive tumors | Genome medicine | 34376232 | Fresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selected from donor peripheral blood using CD56 positivity. Donor NK cell purity Save | Full Paper |
2021 | Steinbuck, MP;Seenappa, LM;Jakubowski, A;McNeil, LK;Haqq, CM;DeMuth, PC; | A lymph node-targeted Amphiphile vaccine induces potent cellular and humoral immunity to SARS-CoV-2 | Science advances | 33547083 | scent serum/plasma Convalescent serum samples (n = 7) and plasma samples (n = 15) from patients who had recovered from SARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and AllCells (Alameda, CA), respectively. All samples | Full Paper |
2021 | Lin, Y;Cui, C;Su, M;Silbart, LK;Liu, H;Zhao, J;He, L;Huang, Y;Xu, D;Wei, X;Du, Q;Lai, L; | Identification of TAPBPL as a novel negative regulator of T-cell function | EMBO molecular medicine | 33938620 | itro T‐cell assays Normal human peripheral blood CD3+ Pan T cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan‐T labeling system were purchased from ALLCELLS, LLC (Alameda, CA). Murine CD3+ T cells were pu | Full Paper |
2021 | Kim, WT;Hennick, K;Johnson, J;Finnerty, B;Choo, S;Short, SB;Drubin, C;Forster, R;McMaster, ML;Hockemeyer, D; | Cancer-associated POT1 mutations lead to telomere elongation without induction of a DNA damage response | The EMBO journal | 33934394 | e aging or age‐associated diseases (Chakravarti et al, 2021). Materials and Methods Cell culture and cryopreservation Human bone marrow CD34+ hematopoietic stem/progenitor cells were obtained from AllCells and Fred Hutchinson Cancer Research Center. | Full Paper |
2021 | Deegen, P;Thomas, O;Nolan-Stevaux, O;Li, S;Wahl, J;Bogner, P;Aeffner, F;Friedrich, M;Liao, MZ;Matthes, K;Rau, D;Rattel, B;Raum, T;Kufer, P;Coxon, A;Bailis, JM; | The PSMA-targeting Half-life Extended BiTE Therapy AMG 160 has Potent Antitumor Activity in Preclinical Models of Metastatic Castration-resistant Prostate Cancer | Clinical cancer research : an official journal of the American Association for Cancer Research | 33504551 | Human T cells were obtained from AllCells. Human peripheral blood mononuclear cells (PBMC) were isolated from buffy coats collected at the Institute of Clinical Transfusion Medicine Save | Full Paper |
2021 | Giffin, MJ;Cooke, K;Lobenhofer, EK;Estrada, J;Zhan, J;Deegen, P;Thomas, M;Murawsky, CM;Werner, J;Liu, S;Lee, F;Homann, O;Friedrich, M;Pearson, JT;Raum, T;Yang, Y;Caenepeel, S;Stevens, J;Beltran, PJ;Canon, J;Coxon, A;Bailis, JM;Hughes, PE; | AMG 757, a Half-Life Extended, DLL3-Targeted Bispecific T-Cell Engager, Shows High Potency and Sensitivity in Preclinical Models of Small-Cell Lung Cancer | Clinical cancer research : an official journal of the American Association for Cancer Research | 33203642 | human CD3+ T cells (2 × 107) by intravenous infusion on study day 0. Human CD3+ T cells, isolated from PBMCs from two different donors using negative selection, were purchased from AllCells and activated in vitro using IL2 and CD3/CD28/CD2 (STEMCELL | Full Paper |
2021 | Kögler, AC;Kherdjemil, Y;Bender, K;Rabinowitz, A;Marco-Ferreres, R;Furlong, EEM; | Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryos | Developmental cell | 34363757 | were also taken from regions not overlapping any mCherry-expressing cells (background (BG)) and from regions overlapping the entire group of cells investigated (AllCells) to Save;each time point, the same measurements were also taken from regions n | Full Paper |
2021 | Montalban-Bravo, G;Kanagal-Shamanna, R;Darbaniyan, F;Siddiqui, MT;Sasaki, K;Wei, Y;Yang, H;Chien, KS;Naqvi, K;Jabbour, E;Kadia, TM;Daver, N;DiNardo, C;Ravandi, F;Pemmaraju, N;Bose, P;Verstovsek, S;Pierce, S;Bueso-Ramos, C;Patel, K;Do, KA;Kantarjian, H;Garcia-Manero, G; | Clinical, genomic, and transcriptomic differences between myelodysplastic syndrome/myeloproliferative neoplasm with ring sideroblasts and thrombocytosis (MDS/MPN-RS-T) and myelodysplastic syndrome with ring sideroblasts (MDS-RS) | American journal of hematology | 33811786 | seven patients with MDS-RS, and 17 healthy individuals obtained from AllCells (Emeryville, CA) were isolated using the CD34 MicroBead Kit and RNA was isolated using the PicoPure RNA isolation kit, following manufacturer's instructions. Fastq files we | Full Paper |
2021 | Kwee, BJ;Lam, J;Akue, A;KuKuruga, MA;Zhang, K;Gu, L;Sung, KE; | Functional heterogeneity of IFN-γ-licensed mesenchymal stromal cell immunosuppressive capacity on biomaterials | Proceedings of the National Academy of Sciences of the United States of America | 34446555 | MSCs obtained from AllCells and Lonza cultured on the two hydrogels (SI Appendix Overall similar observations were obtained with a representative MSC line obtained from AllCells ( Save | Full Paper |
2021 | Cui, L;Moraga, I;Lerbs, T;Van Neste, C;Wilmes, S;Tsutsumi, N;Trotman-Grant, AC;Gakovic, M;Andrews, S;Gotlib, J;Darmanis, S;Enge, M;Quake, S;Hitchcock, IS;Piehler, J;Garcia, KC;Wernig, G; | Tuning MPL signaling to influence hematopoietic stem cell differentiation and inhibit essential thrombocythemia progenitors | Proceedings of the National Academy of Sciences of the United States of America | 33384332 | Healthy deidentified human bone marrow samples were acquired from AllCells. Essential thrombocytosis bone marrow samples were deidentified and acquired from the Stanford Save | Full Paper |
2021 | Poussin, M;Sereno, A;Wu, X;Huang, F;Manro, J;Cao, S;Carpenito, C;Glasebrook, A;Powell, DJ;Demarest, S; | Dichotomous impact of affinity on the function of T cell engaging bispecific antibodies | Journal for immunotherapy of cancer | 34253637 | w cytometric evaluation of cell surface proteins on T cells 48 hours after mixing SKOV3 cells and biologic test articles is further described in online supplemental methods. Briefly, human T cells (ALLCELLS frozen normal human peripheral blood CD3+ P | Full Paper |
2021 | Roselli, E;Boucher, JC;Li, G;Kotani, H;Spitler, K;Reid, K;Cervantes, EV;Bulliard, Y;Tu, N;Lee, SB;Yu, B;Locke, FL;Davila, ML; | 4-1BB and optimized CD28 co-stimulation enhances function of human mono-specific and bi-specific third-generation CAR T cells | Journal for immunotherapy of cancer | 34706886 | Human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells. T cells were enriched from PBMCs using the EasySep human T cell isolation kit according to Save | Full Paper |
2021 | Paavola, KJ;Roda, JM;Lin, VY;Chen, P;O'Hollaren, KP;Ventura, R;Crawley, SC;Li, B;Chen, HH;Malmersjö, S;Sharkov, NA;Horner, G;Guo, W;Kutach, AK;Mondal, K;Zhang, Z;Lichtman, JS;Song, C;Rivera, LB;Liu, W;Luo, J;Wang, Y;Solloway, MJ;Allan, BB;Kekatpure, A;Starck, SR;Haldankar, R;Fan, B;Chu, C;Tang, J;Molgora, M;Colonna, M;Kaplan, DD;Hsu, JY; | The Fibronectin-ILT3 Interaction Functions as a Stromal Checkpoint that Suppresses Myeloid Cells | Cancer immunology research | 34426457 | Human peripheral blood mononuclear cells (PBMC) of healthy donors were isolated from freshly drawn leukopaks (Allcells, catalog no. PB007) by density gradient centrifugation over Save | Full Paper |
2021 | Badeaux, MD;Rolig, AS;Agnello, G;Enzler, D;Kasiewicz, MJ;Priddy, L;Wiggins, JF;Muir, A;Sullivan, MR;Van Cleef, J;Daige, C;Vander Heiden, MG;Rajamanickam, V;Wooldridge, JE;Redmond, WL;Rowlinson, SW; | Arginase Therapy Combines Effectively with Immune Checkpoint Blockade or Agonist Anti-OX40 Immunotherapy to Control Tumor Growth | Cancer immunology research | 33500272 | Primary human bone marrow (AllCells) was obtained from healthy patients and cultured as follows. Briefly, Hs5 cells were cultured for 72 hours in SFEM2 media (Stem Cell Save | Full Paper |
2021 | Boucher, JC;Li, G;Kotani, H;Cabral, ML;Morrissey, D;Lee, SB;Spitler, K;Beatty, NJ;Cervantes, EV;Shrestha, B;Yu, B;Kazi, A;Wang, X;Sebti, SM;Davila, ML; | CD28 Costimulatory Domain-Targeted Mutations Enhance Chimeric Antigen Receptor T-cell Function | Cancer immunology research | 33188139 | An obstacle to the development of chimeric antigen receptor (CAR) T cells is the limited understanding of CAR T-cell biology and the mechanisms behind their antitumor activity. We and Save | Full Paper |
2021 | Li, J;Whelan, S;Kotturi, MF;Meyran, D;D'Souza, C;Hansen, K;Liang, S;Hunter, J;Trapani, JA;Neeson, PJ; | PVRIG is a novel natural killer cell immune checkpoint receptor in acute myeloid leukemia | Haematologica | 33147937 | nt clinical characteristics are summarised in the Online Supplementary Table S2. Healthy donor bone marrow samples were obtained from Royal Melbourne Hospital (Melbourne, Australia) or purchased from AllCells (Alameda, California). All bone marrow sa | Full Paper |
2021 | Gilmartin, AG;Groy, A;Gore, ER;Atkins, C;Long, ER;Montoute, MN;Wu, Z;Halsey, W;McNulty, DE;Ennulat, D;Rueda, L;Pappalardi, M;Kruger, RG;McCabe, MT;Raoof, A;Butlin, R;Stowell, A;Cockerill, M;Waddell, I;Ogilvie, D;Luengo, J;Jordan, A;Benowitz, AB; | In vitro and in vivo induction of fetal hemoglobin with a reversible and selective DNMT1 inhibitor | Haematologica | 32586904 | Cryopreserved human bone marrow CD34+ cells (AllCells) were confirmed to be sourced ethically, and their research use was in accord with the terms of the informed consents under Save | Full Paper |
2021 | Zhang, C;Zhang, X;Huang, L;Guan, Y;Huang, X;Tian, XL;Zhang, L;Tao, W; | ATF3 drives senescence by reconstructing accessible chromatin profiles | Aging cell | 33539668 | accessibility in IARs. Accordingly, our research provides a target to aim for in the exploration of senescence and aging. 4 EXPERIMENTAL PROCEDURES 4.1 Cell culture We purchased primary HUVECs from AllCells Biotech Shanghai Co., Ltd., and cultured t | Full Paper |
2021 | Nesterenko, PA;McLaughlin, J;Tsai, BL;Burton Sojo, G;Cheng, D;Zhao, D;Mao, Z;Bangayan, NJ;Obusan, MB;Su, Y;Ng, RH;Chour, W;Xie, J;Li, YR;Lee, D;Noguchi, M;Carmona, C;Phillips, JW;Kim, JT;Yang, L;Heath, JR;Boutros, PC;Witte, ON; | HLA-A∗02:01 restricted T cell receptors against the highly conserved SARS-CoV-2 polymerase cross-react with human coronaviruses | Cell reports | 34919800 | Cryo preserved peripheral blood mononuclear cells (PBMCs) were commercially purchased (Allcells and Hemacare) or obtained from the CFAR Virology Core Laboratory at the UCLA Save;d virus strains ------------------------- NFAT-zsGreen virus UCLA, | Full Paper |
2021 | Qian, Y;Arellano, G;Ifergan, I;Lin, J;Snowden, C;Kim, T;Thomas, JJ;Law, C;Guan, T;Balabanov, RD;Kaech, SM;Miller, SD;Choi, J; | ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosis | Cell reports | 34433042 | FN-γ, 1 μg/mL anti-IL2 (Cat #554375, clone: S4B6, BD). Isolation of human CD4+ naive T cells and Th cells differentiation Fresh Leukopak samples from de-identified blood donors were purchased from AllCells, LLC. Peripheral blood mononuclear cells (P | Full Paper |
2021 | Hamilton, JR;Tsuchida, CA;Nguyen, DN;Shy, BR;McGarrigle, ER;Sandoval Espinoza, CR;Carr, D;Blaeschke, F;Marson, A;Doudna, JA; | Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineering | Cell reports | 34077734 | ons. Isolation and culture of human primary T cells Primary adult blood cells were obtained from anonymous healthy human donors as a leukoreduction pack purchased from StemCell Technologies, Inc. or Allcells Inc, or as a Trima residual from Vitalant | Full Paper |
2021 | Jiang, Q;Isquith, J;Ladel, L;Mark, A;Holm, F;Mason, C;He, Y;Mondala, P;Oliver, I;Pham, J;Ma, W;Reynoso, E;Ali, S;Morris, IJ;Diep, R;Nasamran, C;Xu, G;Sasik, R;Rosenthal, SB;Birmingham, A;Coso, S;Pineda, G;Crews, L;Donohoe, ME;Venter, JC;Whisenant, T;Mesa, RA;Alexandrov, LB;Fisch, KM;Jamieson, C; | Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolution | Cell reports | 33503434 | Aged normal and MPN Patient samples Obtained through patients consenting at UCSD Moores Cancer Center according to Institutional Review Boardapproved protocols. N/A Young Cord blood CD34+ cells Purchased from AllCells or StemCell Technologies | Full Paper |
2021 | Bunis, DG;Bronevetsky, Y;Krow-Lucal, E;Bhakta, NR;Kim, CC;Nerella, S;Jones, N;Mendoza, VF;Bryson, YJ;Gern, JE;Rutishauser, RL;Ye, CJ;Sirota, M;McCune, JM;Burt, TD; | Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T Cells | Cell reports | 33406429 | Matched adult peripheral blood and BM samples were obtained from healthy volunteer donors through AllCells (Alameda, CA). UCB and longitudinal post-natal samples were obtained Save | Full Paper |
2021 | Yang, Z;Huang, Y;Zhu, L;Yang, K;Liang, K;Tan, J;Yu, B; | SIRT6 promotes angiogenesis and hemorrhage of carotid plaque via regulating HIF-1α and reactive oxygen species | Cell death & disease | 33436551 | oCl2) was purchased from Sigma-Aldrich, Inc. (Burlington, MA, USA). The work concentration and time were confirmed by instructions combined with experimental requirements. HUVECs were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured in | Full Paper |
2021 | Yang, Z;Wei, X;Pan, Y;Xu, J;Si, Y;Min, Z;Yu, B; | A new risk factor indicator for papillary thyroid cancer based on immune infiltration | Cell death & disease | 33414407 | d. The sections were fixed and images were obtained with an inverted microscope (Olympus IX71, Japan). Cell culture and co-culture Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured in | Full Paper |
2021 | Everitt, ML;Boegner, DJ;Birukov, KG;White, IM; | Sample-to-Answer Diagnostic System for the Detection of Circulating Histones in Whole Blood | ACS sensors | 34270219 | Varying concentrations of histone octamers (Sigma-Aldrich) (0-65 μg/mL final concentrations) are suspended in either fetal bovine serum (FBS) (ATCC) or whole blood (AllCells) (medium is indicated in figure caption). Fifteen microliters of histone-spi | Full Paper |
2021 | Varricchio, L;Iancu-Rubin, C;Upadhyaya, B;Zingariello, M;Martelli, F;Verachi, P;Clementelli, C;Denis, JF;Rahman, AH;Tremblay, G;Mascarenhas, J;Mesa, RA;O'Connor-McCourt, M;Migliaccio, AR;Hoffman, R; | TGF-β1 protein trap AVID200 beneficially affects hematopoiesis and bone marrow fibrosis in myelofibrosis | JCI insight | 34383713 | n and response to TGF-β. Methods Further information and complete unedited blots can be found in Supplemental Methods. Human subjects. Primary ND specimens were purchased from a commercial source (AllCells, LLC), whereas MF MNCs were provided by th | Full Paper |
2021 | Pan, Y;You, Y;Sun, L;Sui, Q;Liu, L;Yuan, H;Chen, C;Liu, J;Wen, X;Dai, L;Sun, H; | The STING antagonist H-151 ameliorates psoriasis via suppression of STING/NF-κB-mediated inflammation | British journal of pharmacology | 34460100 | Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells Shanghai and were used immediately after resuscitation. H-151 was synthesized according to the published Save | Full Paper |
2021 | Hu, X;Qie, C;Jiang, J;Xie, X;Chen, W;Liu, W;Liu, J; | M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTA | British journal of pharmacology | 33450048 | Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells and were used immediately after resuscitation; these cells cannot be subcultured. Human CD4 + T cells Save | Full Paper |
2021 | Wang, X;Guo, J;Wen, J;Zhang, X;Cao, L;Zeng, D;Liu, X;Jiang, X; | Novel vascular strategies on polyetheretherketone modification in promoting osseointegration in ovariectomized rats | Materials & Design | HUVECs were purchased from AllCells Biology Technology Company (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, Lonza) without endothelial cell growth supplementation. HUVECs at a density of 2 × 104 cells per mL were seeded o | Full Paper | |
2021 | Iftakhar-E-Khuda, I;Pessia, A;Zheng, S;Kankainen, M;Kontro, M;Karikoski, M;Laurila, J;Gerke, H;Tadayon, S;Hollmén, M;Tang, J;Imhof, BA;Salmi, M;Jalkanen, S; | Vascular adhesion protein-1 defines a unique subpopulation of human hematopoietic stem cells and regulates their proliferation | Cellular and molecular life sciences : CMLS | 34719737 | Five hundred cryopreserved and thawed human BM CD34 + cells (AllCells) were cultured in complete methylcellulose-based medium (H4436, STEMCELL Technologies) with or Save | Full Paper |
2021 | Huang, YJ;Cao, J;Lee, CY;Wu, YM; | Umbilical cord blood plasma-derived exosomes as a novel therapy to reverse liver fibrosis | Stem cell research & therapy | 34772443 | Human UCB and peripheral blood (PB) plasma were purchased from AllCells LLC (Alameda, CA), and exosomes were acquired using ExoQuick Exosome isolation reagent (System Save | Full Paper |
2021 | Pieles, O;Reichert, TE;Morsczeck, C; | Classical isoforms of protein kinase C (PKC) and Akt regulate the osteogenic differentiation of human dental follicle cells via both β-catenin and NF-κB | Stem cell research & therapy | 33853677 | to examine the role of classical PKC isoforms and the potential downstream mechanisms during the osteogenic differentiation of DFCs. Materials and methods Cell culture Human DFCs were purchased from AllCells (Emeryville, USA) and grown in Dulbecco’s | Full Paper |
2021 | Lin, Z;Liu, X;Liu, T;Gao, H;Wang, S;Zhu, X;Rong, L;Cheng, J;Cai, Z;Xu, F;Tan, X;Lv, L;Li, Z;Sun, Y;Qian, Q; | Evaluation of Nonviral piggyBac and lentiviral Vector in Functions of CD19chimeric Antigen Receptor T Cells and Their Antitumor Activity for CD19+ Tumor Cells | Frontiers in immunology | 35082789 | Peripheral blood mononuclear cells (PBMCs) from whole blood were purchased from AllCells (Shanghai, China) and isolated by Ficoll density gradient centrifugation (Cat#17-5442-03, Save | Full Paper |
2021 | Cui, X;Jia, H;Xin, H;Zhang, L;Chen, S;Xia, S;Li, X;Xu, W;Chen, X;Feng, Y;Wei, X;Yu, H;Wang, Y;Zhan, Y;Zhu, X;Zhang, X; | A Novel Bispecific Antibody Targeting PD-L1 and VEGF With Combined Anti-Tumor Activities | Frontiers in immunology | 34925354 | Human PBMCs and DCs were acquired by using the Easy MLR Kit (Allcells, Alameda). DCs were treated with mitomycin (H19999025, Hisun Pharm Co., Ltd.) for 30 min before co- Save | Full Paper |
2021 | Ceglia, V;Kelley, EJ;Boyle, AS;Zurawski, S;Mead, HL;Harms, CE;Blanck, JP;Flamar, AL;Kirschman, JH;Ogongo, P;Ernst, JD;Levy, Y;Zurawski, G;Altin, JA; | A Framework to Identify Antigen-Expanded T Cell Receptor Clusters Within Complex Repertoires | Frontiers in immunology | 34917073 | cificity, are described in other studies (16, 17, 19). Cohesin-Influenza Matrix 1 (Flu M1) protein is described in (16). Donors Cryopreserved human PBMC from normal donors were sourced commercially (AllCells, CA. ND1001 ID:A5983, ND1002 ID:9441, ND1 | Full Paper |
2021 | Teo Hansen Selnø, A;Schlichtner, S;Yasinska, IM;Sakhnevych, SS;Fiedler, W;Wellbrock, J;Berger, SM;Klenova, E;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV; | High Mobility Group Box 1 (HMGB1) Induces Toll-Like Receptor 4-Mediated Production of the Immunosuppressive Protein Galectin-9 in Human Cancer Cells | Frontiers in immunology | 34234778 | Medical Centre Hamburg-Eppendorf (Ethik Kommission der Ärztekammer Hamburg, reference: PV3469). Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were also purchased from AllCells (Alameda, CA, USA) and handled in accordanc | Full Paper |
2021 | Xie, X;Chen, C;Chen, W;Jiang, J;Wang, L;Li, T;Sun, H;Liu, J; | Structural Basis of VSIG3: The Ligand for VISTA | Frontiers in immunology | 33841409 | azole buffer (GenScript). Protein was concentrated using 10K MWCO spin columns (Merck). The purified protein was used for functional verification. Cell Culture and Reagents PBMCs were purchased from Allcells Biotechnology (Shanghai) Co., LTD, and we | Full Paper |
2021 | Jost, M;Jacobson, AN;Hussmann, JA;Cirolia, G;Fischbach, MA;Weissman, JS; | CRISPR-based functional genomics in human dendritic cells | eLife | 33904395 | t al., 2018 Referred to as ‘B. theta 4PP’ in the text Mutant producing tetra-acylated, di-phosphorylated lipid A in a capsule-producing background Biological sample (human) PBMCs AllCells Freshly isolated from de-identified healt | Full Paper |
2021 | Gao, X;Guan, M;Liu, X;Xu, H;Huang, Q;Chen, L;Huang, S;Xiao, Y;Shi, X;Lin, Z; | Sustained delivery of growth factors and alendronate using partially demineralized dentin matrix for endogenous periodontal regeneration | Applied Materials Today | cells (HUVECs) were purchased from AllCells (Shanghai, China). HUVECs were cultured in endothelial cell growth medium (EGM; AllCells, Shanghai, China). Angiogenesis Save | Full Paper | |
2021 | Scherer, SD;Riggio, AI;Haroun, F;DeRose, YS;Ekiz, HA;Fujita, M;Toner, J;Zhao, L;Li, Z;Oesterreich, S;Samatar, AA;Welm, AL; | An immune-humanized patient-derived xenograft model of estrogen-independent, hormone receptor positive metastatic breast cancer | Breast cancer research : BCR | 34717714 | CD34 + hematopoietic stem cells (HSCs) isolated from healthy donor bone marrow (BM) were purchased from AllCells. On the day of injection, cells were thawed in a 37 C water bath Save | Full Paper |
2021 | Nada, MH;Wang, H;Hussein, AJ;Tanaka, Y;Morita, CT; | PD-1 checkpoint blockade enhances adoptive immunotherapy by human Vγ2Vδ2 T cells against human prostate cancer | Oncoimmunology | 34712512 | e IgG1 (clone MOPC-21) antibodies were from Bio X Cell (West Lebanon, NH). Ex vivo expansion of Vγ2Vδ2 T cells by pulse zoledronic acid stimulation PBMC were isolated from random donor leukopaks (AllCells, LLC, Alameda, CA) by density centrifugation | Full Paper |
2021 | Jiang, H;Ni, H;Zhang, P;Guo, X;Wu, M;Shen, H;Wang, J;Wu, W;Wu, Z;Ding, J;Tang, R;Zhou, S;Chen, B;Yu, M;Jing, H;Liu, J; | PD-L1/LAG-3 bispecific antibody enhances tumor-specific immunity | Oncoimmunology | 34239776 | oduced by Innovent Biologics Co., Ltd. (Suzhou, China). Human IgG was purchased from Equitech-Bio (Kerrville). Peripheral blood mononuclear cells (PBMCs) and dendritic cells (DCs) were purchased from AllCells (Shanghai, China). Cynomolgus macaque PBM | Full Paper |
2021 | Wang, X;Peticone, C;Kotsopoulou, E;Göttgens, B;Calero-Nieto, FJ; | Single-cell transcriptome analysis of CAR T-cell products reveals subpopulations, stimulation, and exhaustion signatures | Oncoimmunology | 33489472 | or samples PBMCs were derived from healthy donors leukapheresis. Peripheral blood leukapaheresis were obtained from the NHS as part of a research study (IRAS ID 185945) or purchased as LeukoPaks from AllCells. Manufacturing of CAR T cells product Ge | Full Paper |
2021 | Zhang, C;Delawary, M;Huang, P;Korchak, JA;Suda, K;Zubair, AC; | IL-10 mRNA Engineered MSCs Demonstrate Enhanced Anti-Inflammation in an Acute GvHD Model | Cells | 34831324 | g T7 promoter, 5′ UTRs, 3′ UTRs, and poly-A tail. 2.3. MSC Culture and Time Course Experiment Human MSCs were generated from commercial de-identified bone marrow (Catalog# ABM002) purchased from AllCells (Alameda, CA, USA). The donor was a healthy 2 | Full Paper |
2021 | Ahn, Y;An, JH;Yang, HJ;Lee, DG;Kim, J;Koh, H;Park, YH;Song, BS;Sim, BW;Lee, HJ;Lee, JH;Kim, SU; | Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like System | Cells | 34440805 | Human Induced Pluripotent Stem Cells (hiPSCs) and Culture Conditions The hiPSCs were reprogrammed from primary human bone marrow cells (Allcells, Emeryville, CA, USA) as Save;The hiPSCs were reprogrammed from primary human bone marrow cells (Allcel | Full Paper |
2021 | Dogan, F;Aljumaily, RMK;Kitchen, M;Forsyth, NR; | DNMT3B Is an Oxygen-Sensitive De Novo Methylase in Human Mesenchymal Stem Cells | Cells | 33925659 | nd Methods 2.1. Isolation and Culture of BM-hMSCs Three human BM-hMSCs BMA-16 (Male, Age—29 years, Lonza, MD, USA), BMA-20 (Female, Age—50 years, Lonza, MD, USA) and BMA-25 (Male, Age—27 years, AllCells, LLC, Alameda, CA, USA) were isolated from comm | Full Paper |
2021 | Miao, G;Sun, X; | Development of a novel anti-B7-H4 antibody enhances anti-tumor immune response of human T cells | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | 34328096 | Human PBMCs were purchased from ALLCELLS. Hybridoma cell lines were cultured in H-SFM complete medium. CD8+ T cells were cultured in RPMI 1640 medium containing 2 mM L- Save | Full Paper |
2021 | Gu, C;Cao, X;Wang, Z;Hu, X;Yao, Y;Zhou, Y;Liu, P;Liu, X;Gao, G;Hu, X;Zhang, Y;Chen, Z;Gao, L;Peng, Y;Jia, F;Shan, C;Yu, L;Liu, K;Li, N;Guo, W;Jiang, G;Min, J;Zhang, J;Yang, L;Shi, M;Hou, T;Li, Y;Liang, W;Lu, G;Yang, C;Wang, Y;Xia, K;Xiao, Z;Xue, J;Huang, X;Chen, X;Ma, H;Song, D;Pan, Z;Wang, X;Guo, H;Liang, H;Yuan, Z;Guan, W;Deng, SJ; | A human antibody of potent efficacy against SARS-CoV-2 in rhesus macaques showed strong blocking activity to B.1.351 | mAbs | 34097570 | ed SARS-CoV-2 RBD (Sino Biological, 40592-V05H). Phage libraries were constructed using antibody gene fragments amplified from peripheral blood mononuclear cells (PBMCs) of 50 healthy human subjects (Allcells, PB003F and PB003 C) and total RNA from P | Full Paper |
2021 | Chen, J;Ding, J;Wu, Y;Zhang, S;Zheng, N;Yang, J;Xu, J; | Chromium Oxide Nanoparticle Impaired Osteogenesis and Cellular Response to Mechanical Stimulus | International journal of nanomedicine | 34511912 | Human MSCs were isolated from bone marrow aspirate (ALLCELLS, Lot No: BM2893). The cells were grown in α-MEM (Gibco, Thermo Fisher Scientific, UK) supplemented with 10% Save | Full Paper |
2021 | Shin, Y;Park, CM;Kim, HG;Kim, DE;Choi, MS;Kim, JA;Choi, BS;Yoon, CH; | Identification of Aristolactam Derivatives That Act as Inhibitors of Human Immunodeficiency Virus Type 1 Infection and Replication by Targeting Tat-Mediated Viral Transcription | Virologica Sinica | 32779073 | Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Alameda, CA, USA) and cultured, as described previously (Yoon et al. 2015). HIV-1 clones pNL4-3 and AD8, as well as TZM-bl and A3.01 cells, were obtained from the National Insti | Full Paper |
2021 | Wang, Y;Ni, H;Zhou, S;He, K;Gao, Y;Wu, W;Wu, M;Wu, Z;Qiu, X;Zhou, Y;Chen, B;Pan, D;Huang, C;Li, M;Bian, Y;Yang, M;Miao, L;Liu, J; | Tumor-selective blockade of CD47 signaling with a CD47/PD-L1 bispecific antibody for enhanced anti-tumor activity and limited toxicity | Cancer immunology, immunotherapy : CII | 32761423 | CD4+ T cells were isolated from human PBMC (AllCells) using EasySep human CD4+ T cells enrichment kit (StemCell Technologies). DCs were generated by incubating PBMC first Save | Full Paper |
2021 | Luban, J;Sattler, RA;Mühlberger, E;Graci, JD;Cao, L;Weetall, M;Trotta, C;Colacino, JM;Bavari, S;Strambio-De-Castillia, C;Suder, EL;Wang, Y;Soloveva, V;Cintron-Lue, K;Naryshkin, NA;Pykett, M;Welch, EM;O'Keefe, K;Kong, R;Goodwin, E;Jacobson, A;Paessler, S;Peltz, SW; | The DHODH inhibitor PTC299 arrests SARS-CoV-2 replication and suppresses induction of inflammatory cytokines | Virus research | 33249060 | c. Uridine, brequinar, and A77 were obtained from Sigma-Aldrich (St. Louis, MO). Anti IL-4 and anti-IFL-γ antibodies were obtained from BD Biosciences (San Jose, CA). Frozen PBMCs were obtained from AllCells (Alameda, CA). IL-17, IL-17 F and IL-23 EL | Full Paper |
2021 | Satani, N;Zhang, X;Giridhar, K;Wewior, N;Cai, C;Aronowski, J;Savitz, SI; | A Combination of Atorvastatin and Aspirin Enhances the Pro-Regenerative Interactions of Marrow Stromal Cells and Stroke-Derived Monocytes In Vitro | Frontiers in pharmacology | 33959001 | d of stroke patients. Methods Isolation and Culture of Human Mesenchymal Stromal Cells (MSCs) MSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old male (AllCells, Alameda, CA) using density centrifugation | Full Paper |
2021 | Sun, LK; | Engineering genetic thermal switches for remote control of therapeutic T cells | Thesis | Primary human CD3+ T cells were obtained from anonymous donor blood following apheresis (AllCells) and were cryopreserved until subsequent use. Two days prior to lentiviral Save | Full Paper | |
2021 | Wu, HH;Ralph, KL;Sepuldeva, E;Hansen, G;Li, H;Huang, ZF;Liu, D;Dziegelewski, M;Ahlberg, J;Frego, L;Fogal, S;van Tongeren, S;Grimaldi, C;Litzenberger, T;Presky, D;Singh, S;Brodeur, S;Kroe-Barrett, R; | An optimally designed anti-human CD40 antibody with potent B cell suppression for the treatment of autoimmune diseases | International journal of pharmaceutics | 34624444 | Effector cells were generated by stimulating human peripheral blood mononuclear cells (PBMCs) (AllCells) with 10 ng/mL human IL-2 (R&D Systems) in RPMI 1640 medium (Invitrogen) Save | Full Paper |
2021 | Xiang, H;Chan, AG;Ahene, A;Bellovin, DI;Deng, R;Hsu, AW;Jeffry, U;Palencia, S;Powers, J;Zanghi, J;Collins, H; | Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancer | mAbs | 34719330 | the full-length human FGFR2b described as Ba/F3 FGFR2b, and the effector cells were peripheral blood mononuclear cells obtained fresh from individual human donors (AllCells; Save | Full Paper |
2021 | Li, G;Guo, J;Zheng, Y;Ding, W;Han, Z;Qin, L;Mo, W;Luo, M; | CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T cells | Molecular therapy oncolytics | 34553036 | CAR-T cells were generated from commercial normal donor peripheral blood mononuclear cells (PBMCs) from AllCells. The cells were further enriched from PBMC by CD3 magnetic Save;.22-μm filter, aliquoted to 2 mL cryotubes, quick frozen on dry ice, an | Full Paper |
2021 | Chen, X;Yang, S;Li, S;Qu, Y;Wang, HY;Liu, J;Dunn, ZS;Cinay, GE;MacMullan, MA;Hu, F;Zhang, X;Wang, P; | Secretion of bispecific protein of anti-PD-1 fused with TGF-β trap enhances antitumor efficacy of CAR-T cell therapy | Molecular therapy oncolytics | 33981830 | Frozen human PBMCs were obtained from AllCells. The T cell culture medium (TCM) was composed of AIM-V medium with 5% (v/v) human AB serum, 10 mM HEPES, 1% (v/v) Save;ng gag-pol. The supernatants were harvested at 48 h after transfection and filtere | Full Paper |
2021 | Branquinho, MV;Ferreira, SO;Alvites, RD;Magueta, AF;Ivanov, M;Sousa, AC;Amorim, I;Faria, F;Fernandes, MHV;Vilarinho, PM;Maurício, AC; | In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering-A Preliminary Study | International journal of molecular sciences | 34299274 | e enthalpy of fusion of 100% crystalline material [54]. (1)XC (%)=ΔHfΔHf0×100 4.3. In Vitro Assays 4.3.1. Cell Culture and Maintenance Human Dental Pulp stem/stromal cells (hDPSCs) obtained from AllCells, LLC, Alameda, CA, USA (Cat. DP0037F, Lot no. | Full Paper |
2021 | Thavapalachandran, S;Le, TYL;Romanazzo, S;Rashid, FN;Ogawa, M;Kilian, KA;Brown, P;Pouliopoulos, J;Barry, AM;Fahmy, P;Kelly, K;Kizana, E;Chong, JJH; | Pluripotent stem cell-derived mesenchymal stromal cells improve cardiac function and vascularity after myocardial infarction | Cytotherapy | 34588150 | Cells were kept cold on ice until administration to animals. BM-MSCs were purchased from AllCells (Alameda, CA, USA). Cryopreserved BM-MSCs were washed twice in DMEM and resuspended at the same density as Cymerus MSC derivatives (5 × 106 cells per 10 | Full Paper |
2021 | Kavanagh, H;Dunne, S;Martin, DS;McFadden, E;Gallagher, L;Schwaber, J;Leonard, S;O'Dea, S; | A novel non-viral delivery method that enables efficient engineering of primary human T cells for ex vivo cell therapy applications | Cytotherapy | 33941482 | cells with minimal perturbation of cell function. Methods Ethics All experimental methods were carried out in accordance with the approved guidelines. Leukopaks from healthy donors were sourced from AllCells (Quincy, MA, USA) or BioIVT (Westbury, NY | Full Paper |
2021 | Wang, Y;Zhong, K;Ke, J;Chen, X;Chen, Y;Shu, W;Chen, C;Hu, S;Sun, X;Huang, H;Luo, C;Liu, L;Yang, J;Zhang, Y;Zhi, H; | Combined 4-1BB and ICOS co-stimulation improves anti-tumor efficacy and persistence of dual anti-CD19/CD20 chimeric antigen receptor T cells | Cytotherapy | 33863641 | Human peripheral blood mononuclear cells (PBMCs) from healthy donors were obtained from AllCells. The cryopreserved PBMCs were thawed into the T-cell culture medium and Save | Full Paper |
2021 | Martin, A;Daris, M;Johnston, JA;Cui, J; | HLA-A*02:01-directed chimeric antigen receptor/forkhead box P3-engineered CD4+ T cells adopt a regulatory phenotype and suppress established graft-versus-host disease | Cytotherapy | 33309258 | A*02:01- peripheral blood mononuclear cells (PBMCs) from four donors were isolated from fresh Leukopak (AllCells) using Ficoll density gradient media. CD4+ T cells or Tregs were Save | Full Paper |
2021 | Story, JY;Zoine, JT;Burnham, RE;Hamilton, JAG;Spencer, HT;Doering, CB;Raikar, SS; | Bortezomib enhances cytotoxicity of ex vivo-expanded gamma delta T cells against acute myeloid leukemia and T-cell acute lymphoblastic leukemia | Cytotherapy | 33168453 | PBMCs (50-100 million per donor) from six different healthy donors were obtained through AllCells ( Eight total expansions from PBMCs from AllCells were performed, with two separate Save | Full Paper |
2021 | Banerjee, A;Lu, Y;Do, K;Mize, T;Wu, X;Chen, X;Chen, J; | Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases | Frontiers in cellular neuroscience | 33897370 | ity for polarization. With whole-genome transcript profile, we also demonstrated that iMG cells were clustered closely with human microglia. Materials and Methods Materials PBMCs were purchased from AllCells, LLC (Alameda, CA, USA, #PB003F and #PB00 | Full Paper |
2021 | Stem, C;Rodman, C;Ramamurthy, RM;George, S;Meares, D;Farland, A;Atala, A;Doering, CB;Spencer, HT;Porada, CD;Almeida-Porada, G; | Investigating Optimal Autologous Cellular Platforms for Prenatal or Perinatal Factor VIII Delivery to Treat Hemophilia A | Frontiers in cell and developmental biology | 34447745 | Bone marrow mononuclear cells from two different biological donors were purchased from AllCells (Alameda, CA, United States) and their purchase approved by the Office of Human Save | Full Paper |
2021 | Han, Y;Chen, Q;Zhang, L;Dissanayaka, WL; | Indispensable Role of HIF-1α Signaling in Post-implantation Survival and Angio-/Vasculogenic Properties of SHED | Frontiers in cell and developmental biology | 34368116 | e expression of target proteins that mediate adaptive metabolic mechanisms in both in vivo and in vitro survival and angiogenesis in SHED. Materials and Methods Cell Culture SHED were purchased from AllCells (Alameda, CA, United States) and cultured | Full Paper |
2021 | Yazawa, T;Inaba, H;Imamichi, Y;Sekiguchi, T;Uwada, J;Islam, MS;Orisaka, M;Mikami, D;Ida, T;Sato, T;Miyashiro, Y;Takahashi, S;Khan, MRI;Suzuki, N;Umezawa, A;Kitano, T; | Profiles of 5α-Reduced Androgens in Humans and Eels: 5α-Dihydrotestosterone and 11-Ketodihydrotestosterone Are Active Androgens Produced in Eel Gonads | Frontiers in endocrinology | 33833737 | healthy women volunteers (obtaining informed consent) at University of Fukui Hospital in 2007. Plasma was separated by centrifugation at 1000g for 5 minutes. Other plasma samples were purchased from AllCells (Alameda, CA, USA) and ProMedDX (Norton, M | Full Paper |
2021 | Sternjak, A;Lee, F;Thomas, O;Balazs, M;Wahl, J;Lorenczewski, G;Ullrich, I;Muenz, M;Rattel, B;Bailis, JM;Friedrich, M; | Preclinical Assessment of AMG 596, a Bispecific T-cell Engager (BiTE) Immunotherapy Targeting the Tumor-specific Antigen EGFRvIII | Molecular cancer therapeutics | 33632870 | Human pan-T cells (AllCells) were added at an E:T ratio of 10:1 with a dose range of AMG 596. Microvesicles were added simultaneously with T cells and AMG 596 to maintain Save | Full Paper |
2021 | Massimino, M;Tirrò, E;Stella, S;Manzella, L;Pennisi, MS;Romano, C;Vitale, SR;Puma, A;Tomarchio, C;Di Gregorio, S;Antolino, A;Di Raimondo, F;Vigneri, P; | Impact of the Breakpoint Region on the Leukemogenic Potential and the TKI Responsiveness of Atypical BCR-ABL1 Transcripts | Frontiers in pharmacology | 34276365 | CD34-positive cells derived from healthy donors were obtained from ALLCELLS. CD34-positive cells were maintained in Stem Span SFEM supplemented with cytokines at low Save | Full Paper |
2021 | Tang, J;Zou, Y;Li, L;Lu, F;Xu, H;Ren, P;Bai, F;Niedermann, G;Zhu, X; | BAY 60-6583 Enhances the Antitumor Function of Chimeric Antigen Receptor-Modified T Cells Independent of the Adenosine A2b Receptor | Frontiers in pharmacology | 33776765 | ed States) + 10% FBS (10099141C, ThermoFisher Scientific) were used to expand and culture CAR T cells. Frozen human peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from AllCells (PB005F, Alameda, CA, United States). Aft | Full Paper |
2021 | Sim, DS;Mallari, CR;Teare, JM;Feldman, RI;Bauzon, M;Hermiston, TW; | In vitro characterization of CT-001-a short-acting factor VIIa with enhanced prohemostatic activity | Research and practice in thrombosis and haemostasis | 34263099 | e binding of FVIIa to activated platelet surfaces was detected by Alexa488‐anti‐FVII (clone AA3). Donor informed consent and collection protocols are approved by the Institutional Review Board of AllCells. 2.4 Inhibition by antithrombin and tissue f | Full Paper |
2021 | Xu, Y;Barauskas, O;Kim, C;Babusis, D;Murakami, E;Kornyeyev, D;Lee, G;Stepan, G;Perron, M;Bannister, R;Schultz, BE;Sakowicz, R;Porter, D;Cihlar, T;Feng, JY; | Off-Target In Vitro Profiling Demonstrates that Remdesivir Is a Highly Selective Antiviral Agent | Antimicrobial agents and chemotherapy | 33229429 | Normal human primary bone marrow (BM) light-density cells were from three different lots obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD). All primary human cells Save | Full Paper |
2021 | Huang, Y;Jia, F;Zhao, J;Hou, Y;Hu, SQ; | Novel ACE Inhibitory Peptides Derived from Yeast Hydrolysates: Screening, Inhibition Mechanisms and Effects on HUVECs | Journal of agricultural and food chemistry | 33593053 | Primary HUVECs, as well as the endothelial growth medium (EGM) and endothelial basal serum-free medium, were purchased from Allcells Co., Ltd. (Shanghai, China). Penicillin/ Save | Full Paper |
2021 | Jing, L;Fan, S;Yao, X;Zhang, Y; | Effects of compound stimulation of fluid shear stress plus ultrasound on stem cell proliferation and osteogenesis | Regenerative biomaterials | 34868635 | lopment kit was purchased from Beyotime Biotechnology Co., Ltd. (China). The MagneSil@ Total RNA mini isolation system was purchased from Promega (USA). Primary rat BMSCs were purchased from Shanghai AllCells Biotech Co., Ltd. (China). The polytetraf | Full Paper |
2021 | Chai, ZT;Zhang, XP;Ao, JY;Zhu, XD;Wu, MC;Lau, WY;Sun, HC;Cheng, SQ; | AXL Overexpression in Tumor-Derived Endothelial Cells Promotes Vessel Metastasis in Patients With Hepatocellular Carcinoma | Frontiers in oncology | 34123800 | Primary human umbilical vein endothelial cells (HUVECs; AllCells, Shanghai, China) were cultured in endothelial cell growth medium-2 (Lonza, Basel, Switzerland) supplemented with Save | Full Paper |
2021 | Romito, M;Juillerat, A;Kok, YL;Hildenbeutel, M;Rhiel, M;Andrieux, G;Geiger, J;Rudolph, C;Mussolino, C;Duclert, A;Metzner, KJ;Duchateau, P;Cathomen, T;Cornu, TI; | Preclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV-1 Infection | Biotechnology journal | 33103367 | and data analyzed with CRISPResso2.[49] p values were adjusted with the Benjamini & Hochberg[50] method. For OCA, 1 × 106 PBMCs (ALLCELLS, USA)/mL in X-Vivo15 medium (Lonza), supplemented with 5% human AB serum (Gemini, USA) and 20 ng mL−1 of IL-2 (M | Full Paper |
2021 | Kitanaga, Y;Yamajuku, D;Kubo, S;Nakamura, K;Maeda, M;Seki, M;Kaneko, Y;Kinugasa, F;Morokata, T;Kondo, Y;Yoshinari, H;Nakayamada, S;Sumida, T;Tanaka, Y; | Discovery of a novel Igβ and FcγRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosus | International immunopharmacology | 34781122 | Human peripheral blood mononuclear cells (PBMCs; Lonza, Basel, Switzerland) and normal human peripheral blood B cells (AllCells, CA, USA) were purchased. Whole blood samples were obtained from healthy subjects and patients with SLE at the University | Full Paper |
2021 | Chen, YL;Cui, Y;Liu, X;Liu, G;Dong, X;Tang, L;Hung, Y;Wang, C;Feng, MQ; | A bispecific antibody targeting HER2 and PD-L1 inhibits tumor growth with superior efficacy | The Journal of biological chemistry | 34798072 | man IgG (H + L) was obtained from Jackson ImmunoResearch. Monocyte purification kit was obtained from Miltenyi Biotec. Ficoll gradient was obtained from GE healthcare. Human PBMCs were obtained from Allcells. Humanization of anti-PD-L1 mAb by framew | Full Paper |
2021 | Langrish, CL;Bradshaw, JM;Francesco, MR;Owens, TD;Xing, Y;Shu, J;LaStant, J;Bisconte, A;Outerbridge, C;White, SD;Hill, RJ;Brameld, KA;Goldstein, DM;Nunn, PA; | Preclinical Efficacy and Anti-Inflammatory Mechanisms of Action of the Bruton Tyrosine Kinase Inhibitor Rilzabrutinib for Immune-Mediated Disease | Journal of immunology (Baltimore, Md. : 1950) | 33674445 | Human whole blood from healthy donors was purchased from AllCells and assayed for rilzabrutinib inhibition of IgE basophil activation. Whole blood was treated with ten 3-fold dilutions Save | Full Paper |
2021 | Andrejeva, G;Capoccia, BJ;Hiebsch, RR;Donio, MJ;Darwech, IM;Puro, RJ;Pereira, DS; | Novel SIRPα Antibodies That Induce Single-Agent Phagocytosis of Tumor Cells while Preserving T Cells | Journal of immunology (Baltimore, Md. : 1950) | 33431660 | were differentiated from CD14+ monocytes purchased from Astarte Biologics or selected using Pan Monocyte Isolation Kit (Miltenyi Biotec) from PBMCs (AllCells and Hemacare). Monocytes were seeded onto 96-well, flat-bottom plates at 3 × 104 cells per w | Full Paper |
2021 | Chaerkady, R;Zhou, Y;Delmar, JA;Weng, SHS;Wang, J;Awasthi, S;Sims, D;Bowen, MA;Yu, W;Cazares, LH;Sims, GP;Hess, S; | Characterization of Citrullination Sites in Neutrophils and Mast Cells Activated by Ionomycin via Integration of Mass Spectrometry and Machine Learning | Journal of proteome research | 34008986 | For mast cell preparation, three donors were used. Briefly, leukocytes were collected from Leukopak (ALLCells) and mixed with Robo II Buffer (StemCell Technologies) at 1:4 and centrifuged at 500g for 15 mins. The pellet was resuspended in 1× Vitalyse | Full Paper |
2021 | Deng, M;Wu, D;Zhang, Y;Jin, Z;Miao, J; | MiR-29c downregulates tumor-expressed B7-H3 to mediate the antitumor NK-cell functions in ovarian cancer | Gynecologic oncology | 33875234 | The human CD56 + NK cells were obtained from ALLCELLS (Emeryville, California) and were confirmed by flow cytometric analyses. NK cells were maintained in Human NK cell Save | Full Paper |
2021 | Friedman, D;Dozor, AJ;Milner, J;D'Souza, M;Talano, JA;Moore, TB;Shenoy, S;Shi, Q;Walters, MC;Vichinsky, E;Parsons, SK;Braniecki, S;Moorthy, CR;Ayello, J;Flower, A;Morris, E;Mahanti, H;Fabricatore, S;Klejmont, L;van de Ven, C;Baxter-Lowe, LA;Cairo, MS; | Stable to improved cardiac and pulmonary function in children with high-risk sickle cell disease following haploidentical stem cell transplantation | Bone marrow transplantation | 33958740 | Bioverativ, Sangamo, Veevo, Editas Medicine and is medical director for AllCells, Inc. Biosciences. SKP is a consultant to Seattle Genetics, also unrelated to this study. EV is a consultant for ApoPharma, on the advisory committee for Global Blood Th | Full Paper |
2021 | Song, D;Liu, X;Dong, C;Wang, Q;Sha, C;Liu, C;Ning, Z;Han, J;Liu, H;Zong, M;Zhao, Y;Li, Y;Liu, G;Shao, X;Dou, C; | Two novel human anti-CD25 antibodies with antitumor activity inversely related to their affinity and in vitro activity | Scientific reports | 34824364 | The effector cells PBMCs (PB003F-C, ALLCELLS) were added and the mixture was incubated at 37 C for 5 h. After substrate was added, OD490 value was read on the microplate Save;The effector cells PBMCs (PB003F-C, ALLCELLS) were added and the mixture | Full Paper |
2021 | Loo, J;Sicher, I;Goff, A;Kim, O;Clary, N;Alexeev, A;Sulchek, T;Zamarayeva, A;Han, S;Calero-Garcia, M; | Microfluidic transfection of mRNA into human primary lymphocytes and hematopoietic stem and progenitor cells using ultra-fast physical deformations | Scientific reports | 34725429 | Mobilized peripheral blood CD34-selected hematopoietic stem and progenitor cells (mPB CD34 + HSPCs, purchased from AllCells) were taken from liquid nitrogen freezer and placed Save | Full Paper |
2021 | Wang, X;Negrou, E;Maloney, MT;Bondar, VV;Andrews, SV;Montalban, M;Llapashtica, C;Maciuca, R;Nguyen, H;Solanoy, H;Arguello, A;Przybyla, L;Moerke, NJ;Huntwork-Rodriguez, S;Henry, AG; | Understanding LRRK2 kinase activity in preclinical models and human subjects through quantitative analysis of LRRK2 and pT73 Rab10 | Scientific reports | 34145320 | University of Dundee (https://mrcppureagents.dundee.ac.uk/). For the total Rab10 assay, recombinant Rab10 protein (LSBio, LS-G2178) and Rab8 protein (LS-G29310) were used. Human cryopreserved PBMCs (AllCells, PB005F) were thawed and cultured in RPMI | Full Paper |
2021 | Xue, X;De Leon-Tabaldo, A;Luna-Roman, R;Castro, G;Albers, M;Schoetens, F;DePrimo, S;Devineni, D;Wilde, T;Goldberg, S;Kinzel, O;Hoffmann, T;Fourie, AM;Thurmond, RL; | Preclinical and clinical characterization of the RORγt inhibitor JNJ-61803534 | Scientific reports | 34040108 | mentary materials and methods). Human nTreg suppressive assay Frozen purified human CD4+CD25+ natural Treg cells (nTreg), monocyte-derived dendritic cells (DC) and CD4+CD25- T effector cells (Teff) (Allcells, LLC, Alameda, CA) were thawed and co-cul | Full Paper |
2021 | Harris, KE;Lorentsen, KJ;Malik-Chaudhry, HK;Loughlin, K;Basappa, HM;Hartstein, S;Ahmil, G;Allen, NS;Avanzino, BC;Balasubramani, A;Boudreau, AA;Chang, K;Cuturi, MC;Davison, LM;Ho, DM;Iyer, S;Rangaswamy, US;Sankaran, P;Schellenberger, U;Buelow, R;Trinklein, ND; | A bispecific antibody agonist of the IL-2 heterodimeric receptor preferentially promotes in vivo expansion of CD8 and NK cells | Scientific reports | 34011961 | resuspended in 125 µL/well flow buffer and acquired on a BD FACSCelesta. Whole blood cytokine release assay Cytokine secretion was detected using fresh human whole blood (heparinized) obtained from AllCells. The following method was adapted from B. | Full Paper |
2021 | Tago, Y;Kobayashi, C;Ogura, M;Wada, J;Yamaguchi, S;Yamaguchi, T;Hayashi, M;Nakaishi, T;Kubo, H;Ueda, Y; | Human amnion-derived mesenchymal stem cells attenuate xenogeneic graft-versus-host disease by preventing T cell activation and proliferation | Scientific reports | 33510297 | Human BMSC from healthy donors were purchased from AllCells (CA, USA). The culture conditions of AMSC and BMSC (ie, MSC) were determined based on previous studies 43 . In Save | Full Paper |
2021 | Subhi, H;Husein, A;Mohamad, D;Nik Abdul Ghani, NR;Nurul, AA; | Chitosan-Based Accelerated Portland Cement Promotes Dentinogenic/Osteogenic Differentiation and Mineralization Activity of SHED | Polymers | 34641172 | SHED employed in this study were obtained from ALLCELLS, USA (Catalog No. DP004F; lot number SHED092211-01). Upon being received, the cells were characterized using stem Save | Full Paper |
2021 | Kim, OH;Park, JH;Son, JI;Yoon, OJ;Lee, HJ; | Bone Marrow Mesenchymal Stromal Cells on Silk Fibroin Scaffolds to Attenuate Polymicrobial Sepsis Induced by Cecal Ligation and Puncture | Polymers | 33946773 | y (FE-SEM; JSM 7500F, JEOL Ltd., Akishima, Tokyo, Japan). 2.3. MSC Preparation Human bone marrow MSCs were derived from the whole bone marrow of independent human donors (commercially available from AllCells, Alameda, CA, USA). The cells were cultur | Full Paper |
2021 | Wang, X;Sandberg, ML;Martin, AD;Negri, KR;Gabrelow, GB;Nampe, DP;Wu, ML;McElvain, ME;Toledo Warshaviak, D;Lee, WH;Oh, J;Daris, ME;Chai, F;Yao, C;Furney, J;Pigott, C;Kamb, A;Xu, H; | Potent, Selective CARs as Potential T-Cell Therapeutics for HPV-positive Cancers | Journal of immunotherapy (Hagerstown, Md. : 1997) | 34432728 | For human peripheral blood mononuclear cells (PBMCs), collection protocols and donor informed-consent were approved by an Institutional Review Board at Allcells. Allcells followed Save | Full Paper |
2021 | Martin, AD;Wang, X;Sandberg, ML;Negri, KR;Wu, ML;Toledo Warshaviak, D;Gabrelow, GB;McElvain, ME;Lee, B;Daris, ME;Xu, H;Kamb, A; | Re-examination of MAGE-A3 as a T-cell Therapeutic Target | Journal of immunotherapy (Hagerstown, Md. : 1997) | 33284140 | purchased from Allcells according to the method described by Garcia et al31 Collection protocols and donor informed-consent were approved by an institutional review board at Allcells. Save | Full Paper |
2021 | Xu, F;Liu, X;Zhang, D;Zhao, F;Fan, Z;Hu, S;Mei, S;Huang, Y;Sun, H;Wei, L;Guo, L;Wang, J;Cen, S;Liang, C;Guo, F; | The Engineered MARCH8-Resistant Vesicular Stomatitis Virus Glycoprotein Enhances Lentiviral Vector Transduction | Human gene therapy | 33678011 | Peripheral blood mononuclear cells (PBMCs) isolated from healthy donors were purchased from AllCells (Shanghai, China), and cultured in RPMI 1640 media containing 10% FBS ( Save | Full Paper |
2021 | Chen, Z;Chen, Z;Xu, S;Zhang, Q; | LncRNA SOX2-OT/miR-30d-5p/PDK1 Regulates PD-L1 Checkpoint Through the mTOR Signaling Pathway to Promote Non-small Cell Lung Cancer Progression and Immune Escape | Frontiers in Genetics | The microporous 2-compartment co-culture system was applied for co-culture. Transfected NSCLC cells were inoculated in the upper chamber, while CD8+ T (PB009-3-C, ALLCELLS, Shanghai) cells were inoculated in the lower chamber, allowing direct contact | Full Paper | |
2021 | Raskin, S;Van Pelt, S;Toner, K;Balakrishnan, PB;Dave, H;Bollard, CM;Yvon, E; | Novel TCR-like CAR-T cells targeting an HLA∗0201-restricted SSX2 epitope display strong activity against acute myeloid leukemia | Molecular therapy. Methods & clinical development | 34729377 | Steady-state apheresis units were purchased from AllCells and peripheral blood mononuclear cells (PBMCs) were isolated using lymphoprep density gradient. Before cryopreserving Save;ng a phase I clinical trial to evaluate the safety and anti-tumor e | Full Paper |
2021 | Xue, L;Gao, X;Zhang, H;Tang, J;Wang, Q;Li, F;Li, X;Yu, X;Lu, Z;Huang, Y;Tang, R;Yang, W; | Antiangiogenic antibody BD0801 combined with immune checkpoint inhibitors achieves synergistic antitumor activity and affects the tumor microenvironment | BMC cancer | 34686154 | #0025), 1% penicillin/streptomycin (GIBCO, Invitrogen Inc., Carlsbad, CA, USA, #15140-122) and 1% ECGS (ScienCell, #1052). For HUVEC Western blot, migration inhibition, and scratch assays, HUVECs (AllCells, Alameda, CA, USA) were maintained in HUVEC | Full Paper |
2021 | Noaks, E;Peticone, C;Kotsopoulou, E;Bracewell, DG; | Enriching leukapheresis improves T cell activation and transduction efficiency during CAR T processing | Molecular therapy. Methods & clinical development | 33718517 | Peripheral blood leukapheresis products were obtained from the NHS or purchased as LeukoPaks from AllCells, Cellex, and Anthony Nolan as part of research study NREC ID 15/LO/ Save;omplex and expensive magnetic cell sorting. Materials and methods Cu | Full Paper |
2021 | Green, R;Elliott, JL;Norona, W;Go, F;Nguyen, VT;Ge, J;Short, ML;Mulero, JJ;Zhong, C; | Developmental validation of VeriFiler™ Plus PCR Amplification Kit: A 6-dye multiplex assay designed for casework samples | Forensic science international. Genetics | 33740707 | Human genomic DNA used for the degraded DNA experiments was extracted from normal peripheral blood mononuclear cells (PBMC) of a male donor (ALLCELLS, Emeryville, CA) using a QiAamp DNA Blood Maxi Kit (Qiagen) | Full Paper |
2021 | Park, S;Huang, NWY;Wong, CXY;Pan, J;Albakr, L;Gu, J;Kang, L; | Microstructured Hyaluronic Acid Hydrogel for Tooth Germ Bioengineering | Gels (Basel, Switzerland) | 34449604 | ovine serum (Thermo Fisher, Waltham, MA, USA), penicillin-Streptomycin solution with 10,000 units penicillin and 10 mg streptomycin / mL (Thermo Fisher, Waltham, MA, USA). The culture media for DPSC (AllCells, Alameda, CA, USA) were α-Minimum essenti | Full Paper |
2021 | Garbuzova-Davis, S;Boccio, KJ;Llauget, A;Shell, R;Hailu, S;Mustafa, H;Ehrhart, J;Sanberg, PR;Appel, SH;Borlongan, CV; | Beneficial Effects of Transplanted Human Bone Marrow Endothelial Progenitors on Functional and Cellular Components of Blood-Spinal Cord Barrier in ALS Mice | eNeuro | 34479980 | Cryopreserved human bone marrow CD34+ cells (hBM34+) and human bone marrow-derived endothelial progenitor cells (hBM-EPCs) were purchased from AllCells and CELPROGEN Save | Full Paper |
2021 | Chu, J;Pieles, O;Pfeifer, CG;Alt, V;Morsczeck, C;Docheva, D; | Dental follicle cell differentiation towards periodontal ligament-like tissue in a self-assembly three-dimensional organoid model | European cells & materials | 34251657 | according to AllCells company regulations, further donor information such gender and exact age was not provided to the purchaser). The PDL-hTERT immortal cell line has already Save | Full Paper |
2021 | Madanagopal, TT;Tai, YK;Lim, SH;Fong, CH;Cao, T;Rosa, V;Franco-Obregón, A; | Pulsed electromagnetic fields synergize with graphene to enhance dental pulp stem cell-derived neurogenesis by selectively targeting TRPC1 channels | European cells & materials | 33644848 | hDPSC culture The use of human cells was approved by the NUS Institutional Review Board (NUS2094/2015). hDPSC (DPF003, AllCells, Quincy, MA, USA) were cultured in basal growth medium (DMEM, Invitrogen), supplemented with 10 % FBS (Invitrogen) in the | Full Paper |
2021 | Wang, X;Martin, AD;Negri, KR;McElvain, ME;Oh, J;Wu, ML;Lee, WH;Ando, Y;Gabrelow, GB;Toledo Warshaviak, D;Sandberg, ML;Xu, H;Kamb, A; | Extensive functional comparisons between chimeric antigen receptors and T cell receptors highlight fundamental similarities | Molecular immunology | 34419823 | Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols Save | Full Paper |
2021 | Zacharia, A;Harberts, E;Valencia, SM;Myers, B;Sanders, C;Jain, A;Larson, NR;Middaugh, CR;Picking, WD;Difilippantonio, S;Kirnbauer, R;Roden, RB;Pinto, LA;Shoemaker, RH;Ernst, RK;Marshall, JD; | Optimization of RG1-VLP vaccine performance in mice with novel TLR4 agonists | Vaccine | 33309485 | cells were also cultured in the presence of BECC470 or TLR4 agonists and the resulting upregulation of surface co-stimulatory markers was measured by flow cytometry (AllCells). Save | Full Paper |
2021 | Liu, C;Yang, G;Zhou, M;Zhang, X;Wu, X;Wu, P;Gu, X;Jiang, X; | Magnesium Ammonium Phosphate Composite Cell-Laden Hydrogel Promotes Osteogenesis and Angiogenesis In Vitro | ACS omega | 33869925 | Human umbilical vein endothelial cells (HUVECs; eahy926) were purchased from AllCells and cultured in EBM. They were seeded in the upper chamber, while the lower chambers Save | Full Paper |
2021 | Jangjoo, M;Goodman, SJ;Choufani, S;Trost, B;Scherer, SW;Kelley, E;Ayub, M;Nicolson, R;Georgiades, S;Crosbie, J;Schachar, R;Anagnostou, E;Grunebaum, E;Weksberg, R; | An Epigenetically Distinct Subset of Children With Autism Spectrum Disorder Resulting From Differences in Blood Cell Composition | Frontiers in neurology | 33935932 | ed, B cell, CD4T, CD8T, monocyte, granulocytes, and NK. These blood cell types were those on which the Houseman method was originally trained, using peripheral blood leukocyte subtypes purchased from AllCells , LLC (Emeryville, CA) or sorted cells fr | Full Paper |
2021 | Zhou, N;Chen, Y;Yang, L;Xu, T;Wang, F;Chen, L;Liu, J;Liu, G; | LncRNA SNHG4 promotes malignant biological behaviors and immune escape of colorectal cancer cells by regulating the miR-144-3p/MET axis | American journal of translational research | 34786048 | The transfected CACO2 cells were co-cultured with CD4+ T cells (PB009-2-C, AllCells Biotechnology Co., Ltd., Shanghai, China), followed by the addition of PD-1 antibody (ab52587, Abcam, UK) and PD-L1 antibody (ab205921, Abcam, UK). CD4+ T cell isolat | Full Paper |
2021 | Horton, TM;Hoff, FW;van Dijk, A;Jenkins, GN;Morrison, D;Bhatla, T;Hogan, L;Romanos-Sirakis, E;Meyer, J;Carroll, WL;Qiu, Y;Wang, T;Mo, Q;Kornblau, SM; | The effects of sample handling on proteomics assessed by reverse phase protein arrays (RPPA): Functional proteomic profiling in leukemia | Journal of proteomics | 33212251 | Normalization controls were commercially available normal adult CD34+ bone marrow cells (AllCells). Peripheral blood mononuclear cells (PBMC) were isolated using Lymphoprep Save | Full Paper |
2021 | Khouri, J;Faiman, BM;Grabowski, D;Mahfouz, RZ;Khan, SN;Wei, W;Valent, J;Dean, R;Samaras, C;Jha, BK;Lazarus, H;Campagnaro, EL;Malek, E;Reed, J;Karam, MA;Hamilton, K;Fada, S;Kalaycio, M;Liu, H;Sobecks, R;Saunthararajah, Y;Chew, Y;Orloff, M;Reu, FJ; | DNA methylation inhibition in myeloma: Experience from a phase 1b study of low-dose continuous azacitidine in combination with lenalidomide and low-dose dexamethasone in relapsed or refractory multiple myeloma | Seminars in hematology | 33509443 | Normal bone marrow mononuclear cells for separation of CD138 positive and negative fractions of 3 healthy donors was obtained commercially from ALLCELLS and processed the Save | Full Paper |
2021 | Schölzel, C;Blesius, V;Ernst, G;Goesmann, A;Dominik, A; | Countering reproducibility issues in mathematical models with software engineering techniques: A case study using a one-dimensional mathematical model of the atrioventricular node | PloS one | 34280231 | This means that the release version of the AllCells model is not only executable in OpenModelica, but also in any of the over 100 tools that support the Functional Mock-up Interface (FMI Save | Full Paper |
2021 | Dulay, RM;Valdez, BC;Li, Y;Chakrabarti, S;Dhillon, B;Kalaw, SP;Reyes, RG;Cabrera, EC; | Cytotoxicity of Gymnopilus purpureosquamulosus extracts on hematologic malignant cells through activation of the SAPK/JNK signaling pathway | PloS one | 34048499 | esuspended in freezing medium (50% heat-inactivated fetal bovine serum, 40% RPMI, 10% DMSO), and cryopreserved in liquid nitrogen until used. Mononuclear cells from healthy donors were purchased from ALLCELLS LLC (Almeda, CA, USA). For extract exposu | Full Paper |
2021 | Caplan, HW;Prabhakara, KS;Toledano Furman, NE;Zorofchian, S;Martin, C;Xue, H;Olson, SD;Cox, CS; | Human-derived Treg and MSC combination therapy may augment immunosuppressive potency in vitro, but did not improve blood brain barrier integrity in an experimental rat traumatic brain injury model | PloS one | 34038436 | Bone marrow-derived MSC were isolated from a commercially available fresh human bone marrow aspirate (AllCells, Alameda, CA) and expanded following established procedures [8, Save | Full Paper |
2021 | Baik, R;Wyman, SK;Kabir, S;Corn, JE; | Genome editing to model and reverse a prevalent mutation associated with myeloproliferative neoplasms | PloS one | 33661998 | Frozen human mobilized peripheral blood CD34+ HSPCs were purchased from AllCells and thawed according to manufacturer's instructions. CD34+ cells were cultured in StemSpan Save | Full Paper |
2021 | Garbuzova-Davis, S;Boccio, KJ;Ehrhart, J;Sanberg, PR;Appel, SH;Borlongan, CV; | Detection of endothelial cell-associated human DNA reveals transplanted human bone marrow stem cell engraftment into CNS capillaries of ALS mice | Brain research bulletin | 33545308 | Cryopreserved human bone marrow CD34+ cells (hBM34+) and human bone marrow-derived endothelial progenitor cells (hBM-EPCs) were purchased from AllCells (Alameda, CA, USA) and CELPROGEN (Torrance, CA, USA), respectively. According to the company repor | Full Paper |
2021 | Montalban-Bravo, G;Darbaniyan, F;Kanagal-Shamanna, R;Ganan-Gomez, I;Class, CA;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, KA;Chien, KS;Bueso-Ramos, C;Do, KA;Kantarjian, H;Garcia-Manero, G; | Type I interferon upregulation and deregulation of genes involved in monopoiesis in chronic myelomonocytic leukemia | Leukemia research | 33517186 | BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). Informed consent was obtained according to protocols approved by the MDACC institutional review Save | Full Paper |
2021 | Lu, J;Collins, P;Lee, KJ;Li, CM;Wang, S; | In vitro assessment of an engineered tBID-based safety switch system in human T lymphocytes | Annals of translational medicine | 34988150 | n 12-well plate and incubated the plate in incubator at 37 °C. Human T-cell samples were purchased anonymously from the vendor and considered exempt from IRB approval. The frozen human pan-T cells (AllCells, LLC., Cat# S-ISO004-06) were thawed and c | Full Paper |
2021 | Maucort, C;Di Giorgio, A;Azoulay, S;Duca, M; | Differentiation of Cancer Stem Cells by Using Synthetic Small Molecules: Toward New Therapeutic Strategies against Therapy Resistance | ChemMedChem | 32803855 | In an analogous work, it was found that ICG-001 could safely eradicate the drug-resistant CSC-like population and initiate cell differentiation in leukemia (pre-B ALLcells). The same Save | Full Paper |
2021 | Hu, J;Zhang, J;Yu, M;Liu, Z;Zou, Y;Hong, L;Zhang, T;Sun, J;Zheng, M;Zhu, X;Wang, Z;Liu, S; | Circulating miR-221/222 reduces CD4+ T cells by inhibiting CD4 expression in colorectal cancer | Acta biochimica et biophysica Sinica | 34357372 | Peripheral blood mononuclear cells (PBMCs) were purchased from ALLCELLS (PB005F; Shanghai, China). PBMCs were incubated with DynaBeads (11141D; Gibco, Carlsbad, USA), and CD3+ T cells were isolated in a magnet by binding to DynaBeads containing anti- | Full Paper |
2021 | Yang, Y;Wang, C;Dai, C;Liu, X;Li, W;Huang, M;Zhao, X;Ji, D;Li, J;Guo, W; | Amplification and expression of c-MET correlate with poor prognosis of patients with gastric cancer and upregulate the expression of PDL1 | Acta biochimica et biophysica Sinica | 33693450 | The prognostic significance of c-MET in gastric cancer (GC) remains uncertain. In the present study, we examined the amplification, expression, and the prognostic value of c-MET, Save | Full Paper |
2021 | Thorarensen, A;Balbo, P;Banker, ME;Czerwinski, RM;Kuhn, M;Maurer, TS;Telliez, JB;Vincent, F;Wittwer, AJ; | The advantages of describing covalent inhibitor in vitro potencies by IC50 at a fixed time point. IC50 determination of covalent inhibitors provides meaningful data to medicinal chemistry for SAR optimization | Bioorganic & medicinal chemistry | 33285410 | Cryopreserved human PBMCs (Catalog No. PB005F), which were used in the IL-2, IL-10, IL-15, and IFNα assays, were purchased from Allcells (Emeryville, CA). Frozen PBMCs were thawed in a water bath (37 °C), and washed once with RPMI1640 medium (Catalog | Full Paper |
2021 | Jiang, J;Li, X;Mao, F;Wu, X;Chen, Y; | Small molecular fluorescence dyes for immuno cell analysis | Analytical biochemistry | 33306976 | Statement of Ethical Use of Human Samples: All human PBMCs used in this study were obtained from AllCells Alameda, 1301 Harbor Bay Parkway, Suite 200, Alameda, CA 94502, Save;h as leukemia; and provide a quick and efficient way to study the immune | Full Paper |
2021 | Takahashi, N;Higa, A;Hiyama, G;Tamura, H;Hoshi, H;Dobashi, Y;Katahira, K;Ishihara, H;Takagi, K;Goda, K;Okabe, N;Muto, S;Suzuki, H;Shimomura, K;Watanabe, S;Takagi, M; | Construction of in vitro patient-derived tumor models to evaluate anticancer agents and cancer immunotherapy | Oncology letters | 33841567 | patient, were used in this study. Patient-derived hematopoietic tumor cells were provided by Fukushima Medical University Hospital, the Japanese Pediatric Cancer Group ALL-R14 study, ProteoGenex, or AllCells. Peripheral blood mononuclear cells (PBMCs | Full Paper |
2021 | Yoshida, K;Takabayashi, T;Imoto, Y;Sakashita, M;Kato, Y;Narita, N;Fujieda, S; | Increased Thrombin-Activatable Fibrinolysis Inhibitor in Response to Sublingual Immunotherapy for Allergic Rhinitis | The Laryngoscope | 33844301 | Systems, Minneapolis, Minnesota ), 100 ng IL-13 (R&D Systems), 100 ng IL-4 plus 1 μg/mL TAFI (Abcam, Cambridge, UK), and 100 ng IL-13 with 1 μg/mL TAFI. Human mast cells were differentiated from CD34+ hematopoietic progenitor cells (ALLCELLS, Alamed | Full Paper |
2021 | Hashim, SNM;Yusof, MFH;Zahari, W;Noordin, KBAA;Akamatsu, T;Azlina, A; | Amniotic membrane matrix effects on calcineurin-NFAT-related gene expressions of SHED treated with VEGF for endothelial differentiation | In vitro cellular & developmental biology. Animal | 34021476 | SHED (AllCells, Alameda, California) were cultured in a complete medium made of the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, Waltham, Massachusetts), supplemented with 15% of fetal bovine serum (FBS) (Gibco, Waltham, Massachusetts) | Full Paper |
2021 | Pieles, O;Hartmann, M;Morsczeck, C; | AMP-activated protein kinase and the down-stream activated process of autophagy regulate the osteogenic differentiation of human dental follicle cells | Archives of oral biology | 33254047 | 2.1. Cell culture Human dental follicle cells were purchased from AllCells and cultivated in DMEM (Dulbecco’s Modified Eagle’s Medium) high glucose with 10 % FBS (fetal bovine serum) and 1% penicillin (10,000 units/mL) / streptomycin (10 mg/mL) solut | Full Paper |
2021 | Wang, Z;Jiang, L;Wang, J;Chai, Z;Xiong, W; | Morphine promotes angiogenesis by activating PI3K/Akt/HIF-1α pathway and upregulating VEGF in hepatocellular carcinoma | Journal of gastrointestinal oncology | 34532126 | Academy of Science (Shanghai, China). The cells were cultured in 10% FBS DMEM, 37 °C, 5% CO2 incubator. HUVECs were obtained from the Allcells Company(Shanghai, China) and cultured in 10% FBS Endothelial Cell Growth Medium (ECM, Lonza, Switzerland), | Full Paper |
2021 | Hayashi, Y;Kawaki, H;Hori, M;Shintani, K;Hasegawa, T;Tanaka, M;Kondoh, N;Yoshida, T;Kawano, S;Tamaki, Y; | Evaluation of the mechanical properties and biocompatibility of gypsum-containing calcium silicate cements | Dental materials journal | 33642445 | Evaluation of the cytotoxicity of cement-immersed media Samples of hDPSCs (Allcells, Emeryville, CA, USA), which had been subcultured 11-14 times in 10% fetal bovine serum (FBS)-containing α-MEM, were seeded within a 96-well plate at a density of 4,0 | Full Paper |
2021 | Iwasa, M;Fujii, S;Fujishiro, A;Maekawa, T;Andoh, A;Takaori-Kondo, A;Ichinohe, T;Miura, Y; | Impact of 2 Gy γ-irradiation on the hallmark characteristics of human bone marrow-derived MSCs | International journal of hematology | 33386593 | Bone marrow of multiple healthy adults aged 21-44 years (#4362, #4615, and #4641) was purchased from AllCells (Emeryville, CA). Human BM-MSCs were isolated from bone marrow according to our previously published methods [12, 13]. In brief, a single-ce | Full Paper |
2021 | Cui, L;Yin, F;Cheng, J;Liu, H;Zheng, M;Liu, D;Wu, Z;Qian, Q; | Optimized cytotoxicity assay for co-suspended effector and target cells | Journal of immunological methods | 34270976 | Peripheral blood mononuclear cells (PBMCs) obtained from whole blood of healthy volunteers (AllCells, Silicon Valley, CA, USA) were isolated by density gradient centrifugation with Save | Full Paper |
2021 | Sunderic, K;Li, C;Ahmed, AHR;Dawkins, D;Azar, T;Cardoso, L;Wang, S; | Tuning Thermal Dosage to Facilitate Mesenchymal Stem Cell Osteogenesis in Pro-Inflammatory Environment | Journal of biomechanical engineering | 32601701 | Human bone marrow explanted from the iliac crest of donors was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the RosetteSep Save | Full Paper |
2021 | Koh, H;Kwon, SY;Zhen, X;Ha, HY;Lee, JH; | Generation of induced pluripotent stem cell line (KRIBBi004-A) from adult bone marrow CD34+ cells from a patient carrying 46,XX,t(1;5)(p31.1;35.1) karyotype | Stem cell research | 34736040 | Cell Source iPSC was derived from CD34+ bone marrow (BM) cells, obtained from AllCells, LLC Clonality Clonal Associated disease N/A Gene/locus Chromosome translocation t(1;5)(p31.1;35.1) Date archived/stock date Jun.2021 Cell line repository/bank The | Full Paper |
2021 | Kim, J;Koh, H;Zhen, X;Lee, DS;Ha, HY;Lee, JH; | Establishment of iPSC (KRIBBi001-A) from CD34+ group O D-negative bone marrow blood | Stem cell research | 33529979 | Cell Source iPSC was derived from CD34+ bone marrow (BM) cells, obtained from AllCells, LLC Clonality Clonal Method of reprogramming Integration-free Sendai virus-based vectors (CytoTune™-iPS 2.0 Sendai Reprogramming Kit, Life Technologies) | Full Paper |
2021 | Fu, X;Shen, X;Yin, X;Zhang, Y;Wang, X;Han, Z;Lin, Q;Fan, J; | Antioxidant and pro-apoptosis activities of coffee husk (Coffea arabica) anthocyanins | International Food Research Journal | The HUVEC and Caco-2 cell lines were purchased from ALLCELLS Biotechnology Company (Shanghai, China) and Kunming Institute of Zoology Cell Line Bank (Yunnan, China), respectively. Dulbecco’s Modified Eagle’s Medium (DMEM), foetal bovine serum, penici | Full Paper | |
2021 | Choi, J;Goulding, SP;Conn, BP;McGann, CD;Dietze, JL;Kohler, J;Lenkala, D;Boudot, A;Rothenberg, DA;Turcott, PJ;Srouji, JR;Foley, KC;Rooney, MS;van Buuren, MM;Gaynor, RB;Abelin, JG;Addona, TA;Juneja, VR; | Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutations | Cell reports methods | 35474673 | (HLA-A∗03:01, HLA-A∗11:01, HLA-A∗68:01, HLA-B∗07:02, HLA-C∗01:02, HLA-C∗03:04, and/or HLA-C∗08:02) were isolated using Ficoll separation from apheresis material (AllCells Save;130-050-201; RRID:AB_2665482 CD25 MicroBeads, human Miltenyi Biotec | Full Paper |
2021 | Cai, W;Dong, J;Gallolu Kankanamalage, S;Titong, A;Shi, J;Jia, Z;Wang, B;Huang, C;Zhang, J;Lin, J;Kan, SZ;Han, S;Zhou, J;Liu, Y; | Biological activity validation of a computationally designed Rituximab/CD3 T cell engager targeting CD20+ cancers with multiple mechanisms of action | Antibody therapeutics | 34805746 | They were cultured in RPMI 1640 (Thermo fisher) medium supplemented with 10% fetal bovine serum (FBS) (GIBCO) at 37 °C, 5% CO2. Human peripheral blood mononuclear cells (hPBMC) were purchased from AllCells (Alameda, CA) and HumanCells Bio (San Jose, | Full Paper |
2021 | Moe, GR;Steirer, LM;Lee, JA;Shivakumar, A;Bolanos, AD; | A cancer-unique glycan: de-N-acetyl polysialic acid (dPSA) linked to cell surface nucleolin depends on re-expression of the fetal polysialyltransferase ST8SIA2 gene | Journal of experimental & clinical cancer research : CR | 34544457 | rting (FACS) binding, and fluorescence labeling experiments were treated with Accutase and washed with medium before use. Human normal peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Alameda, CA). Protein extraction from ce | Full Paper |
2021 | Xing, Y;Chu, KA;Wadhwa, J;Chen, W;Zhu, J;Bradshaw, JM;Shu, J;Foulke, MC;Loewenstein, N;Nunn, P;By, K;Phiasivongsa, P;Goldstein, DM;Langrish, CL; | Preclinical Mechanisms of Topical PRN473, a Bruton Tyrosine Kinase Inhibitor, in Immune-Mediated Skin Disease Models | ImmunoHorizons | 34326199 | Human whole blood (AllCells) pretreated for 1 h with eleven 3-fold serial dilutions of PRN473, starting at a concentration of 10 μM, was incubated overnight with goat anti-human IgM F( Save | Full Paper |
2021 | Pohlmeyer, CW;Shang, C;Han, P;Cui, ZH;Jones, RM;Clarke, AS;Murray, BP;Lopez, DA;Newstrom, DW;Inzunza, MD;Matzkies, FG;Currie, KS;Di Paolo, JA; | Characterization of the mechanism of action of lanraplenib, a novel spleen tyrosine kinase inhibitor, in models of lupus nephritis | BMC rheumatology | 33781343 | healthy donors or individuals with SLE (AllCells) were treated with lanraplenib or dimethyl Naive B cells were isolated from freshly collected PBMCs (AllCells) by negative selection ( Save | Full Paper |
2021 | Nordin, NS;Mokhtar, KI;Mustafa, BE; | Effects of Flaxseed (Linum usitatissimum) Extract on the Osteoblast Differentiation Potential of Stem Cells Derived from Human Exfoliated Deciduous Teeth | Makara Journal of Health Research | SHED (ALLCells, USA) were cultured in complete growth media (alpha-MEM; 10% FBS; 0.5% Pen-Strep) until confluence. SHED at 80%-90% confluence were subjected to osteoblast differentiation. The complete growth medium was replaced with osteoblast induct | Full Paper | |
2021 | Turkson, A;Addor, J;Kharib, D; | Validating Intrinsic Factors Informing E-Commerce: Categorical Data Analysis Demo | Open Journal of Statistics | ows a chi-square distribution, and the conclusion depends on whether or not the obtained statistic is greater that the critical statistic at a chosen alpha level. The test statistics is: χ 2 = ∑ allcells ( Observed − Expected ) 2 Expected = ∑ i , j | Full Paper | |
2021 | Xu, Y;Shen, L;Li, G; | The The Effect of Elution Volume for Immunoprecipitation on m6A-Seq Analysis | Proceedings of Anticancer Research | The Mouse Renal Carcinoma cells (RenCa) were cultured in DMEM medium (Invitrogen) containing 10% FBS, 2 mM L-Glutamine and 1% penicillin-streptomycin. RenCa cell line was purchased from Allcells Biotechnology (Shanghai, China) and were routinely test | Full Paper | |
2021 | Zhou, Z;Zheng, J;Lin, D;Chen, Y;Hu, X; | Exosomes Derived from Dental Pulp Stem Cells Accelerate Cutaneous wound Healing by Enhancing Angiogenesis via Cdc42/p38 MAPK Pathway | Research Square | 1% penicillin-streptomycin (Sigma, USA). HUVECs were bought from ALLCELLS (Shanghai, China) and expanded in endothelial cell growth medium (EGM). For exosome co-cultures experiment, 10μg/ml of exosomes were added to the culture medium of HUVECs. All | Full Paper | |
2021 | Blitzer, G;Paz, C;Glassey, A;Ganz, O;Giri, J;Pennati, A;Meyers, R;Lunga, T;Robbins, D;Thibeault, S;Bates, A;Nickel, K;Morris, Z;Mattison, R;Chappell, R;Glazer, T;Rogus-Pulia, N;Galipeau, J;Kimple, R; | A Pilot Study to Assess the Salivary Gland Regenerative Potential of Bone Marrow Mesenchymal Stromal Cells from Treated Head and Neck Cancer Patients | Research Square | Bone marrow from healthy adult control volunteers were purchased from a commercial source (AllCells, Alameda, CA). We had originally planned to enroll 15 patients: 5 patients with Save | Full Paper | |
2021 | Tie, B;Guo, Z;Li, L;Wang, W;Liu, R;Fang, J; | miR-3156-5p Enhances Hypoxia-Induced Angiogenesis in Hepatocellular Carcinoma via Modulating the SOCS5/HIF-1α/VEGF Pathway | Research Square | Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in the medium containing 50 U/mL penicillin and 50 U/mL streptomycin (Invitgen, San Diego, CA), 50 mL endothelial cell growth additive (Upstat | Full Paper | |
2021 | Chen, S;Barrientos, J;Ferrer, G;Ravichandran, P;Ibrahim, M;Kieso, Y;Kutok, J;Peluso, M;Sharma, S;Weaver, D;Pachter, J;Rai, K;Chiorazzi, N; | Duvelisib Eliminates CLL B Cells, Impairs CLL-Supporting Cells, and Overcomes Ibrutinib Resistance in Preclinical Models | Research Square | Human PBMCs from CLL donors obtained from whole blood from a commercial source (Bioreclamation IVT or ALLCELLS) were resuspended (1 x 106 cells/mL) in RPMI-1640 medium Save | Full Paper | |
2021 | Valadkhan, S;Plasek, L;Gunawardane, L;Niazi, F;Mason, S;Leskov, K;Perez, G;Dobrowolski, C;Shukla, M;Nutt, W;Karn, J; | An HIV-induced mechanism for T cell quiescence andproviral latency | Research Square | Peripheral blood mononuclear cells from HIV negative Caucasian male donors were purchased from Allcells. Naive CD4+ T cells were isolated using EasySep Human Naive CD4+ T Save | Full Paper | |
2021 | Cai, W;Dong, J;Kankanamalage, S;Titong, A;Shi, J;Jia, Z;Wang, B;Huang, C;Zhang, J;Lin, J;Kan, S;Zhou, J;Liu, Y; | A computationally designed Rituximab/CD3 T cell engager targeting CD20+ cancers with multiple mechanisms of action | Research Square | medium supplemented with 10% fetal bovine serum (FBS) (GIBCO) 115 at 37°C, 5% CO2. Human peripheral blood mononuclear cells (hPBMC) were purchased from 116 AllCells (Alameda, CA) and HumanCells Bio (San Jose, CA). Rituximab resistant Raji cells 117 ( | Full Paper | |
2021 | Zhao, C;Zhang, W;Gong, G;Xie, L;Wang, M;Hu, Y; | A New Approach to Produce IgG4-like Bispecific Antibodies | Research Square | Human CD4+ T cells were purified from PBMC (AllCells, China) by negative selection with CD4+ T cell enrichment cocktail kit (Stemcell, Vancouver, Canada) according to the manufacturer’s instruction. Immature DC (iDC) was generated from monocytes by c | Full Paper | |
2021 | Noto, P; | Analysis of Liver X Receptor target gene expression across species | Drexel University | cDNA from Human MTC™ Panels I and II were purchased from Clontech (catalog# 636742 and 636743) and subjected to real-time PCR. Whole blood from human donors was purchased from AllCells (catalog# WB001) | Full Paper | |
2021 | Dunbar, A;Kim, D;Lu, M;Farina, M;Yang, J;Park, Y;Gobbo, F;Verachi, P;Martelli, F;Karzai, A;Xiao, W;Xia, L;Elmansy, N;Kleppe, M;Chen, Z;Xiao, Y;McGovern, E;Snyder, J;Krishnan, A;Hill, C;Cordner, K;Zouak, A;Salama, M;Yohai, J;Tucker, E;Chen, J;Zhou, J;McConnell, T;Koche, R;Rampal, R;Migliaccio, A;Fan, R;Levine, R;Hoffman, R; | CXCL8/CXCR2 signaling mediates bone marrow fibrosis and represents a therapeutic target in myelofibrosis | bioRxiv | Pro-inflammatory signaling is a hallmark feature of human cancer, including in myeloproliferative neoplasms (MPNs), most notably myelofibrosis (MF). Dysregulated inflammatory Save | Full Paper | |
2021 | Kartha, V;Duarte, F;Hu, Y;Ma, S;Chew, J;Lareau, C;Earl, A;Burkett, Z;Kohlway, A;Lebofsky, R;Buenrostro, J; | Functional Inference of Gene Regulation using Single-Cell Multi-Omics | bioRxiv | CD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from AllCells (see Table S1 for catalog numbers and donor information). Cells were quickly thawed in a 37°C water bath, rinsed with culture medium (Iscove’s Modified | Full Paper | |
2021 | Kong, T;Laranjeira, A;Yang, K;Fisher, D;Yu, L;Wang, A;Ruzinova, M;Fowles, J;Allen, M;Celik, H;Challen, G;Huang, S;Oh, S; | DUSP6 mediates resistance to JAK2 inhibition and drives leukemic progression | bioRxiv | gradient extraction and cryopreserved according to standard procedures. Additional BMMCs were purchased from AllCells (Alameda, California). A list of patient samples utilized in this study is provided in Table 1 | Full Paper | |
2021 | Qi, J;D’Souza, D;Dawson, T;Geanon, D;Stefanos, H;Marvin, R;Walker, L;Rahman, A; | Multimodal Single-Cell Characterization of the Human Granulocyte Lineage | bioRxiv | Fresh whole bone marrow was purchased from AllCells Inc. and delivered on the day of the experiment. The bone marrow was in a volume of 3mL and the sample was limited to 2 units/ Save | Full Paper | |
2021 | Kim, J;Schulte, A;Sarver, A;Angelos, M;Frantz, A;Forster, C;O’Brien, T;Cornax, I;O’Sullivan, M;Cheng, N;Lewellen, M;Oseth, L;Kumar, S;Bullman, S;Pedamallu, C;Goyal, S;Meyerson, M;Lund, T;Alfoldi, J;Breen, M;Lindblad-Toh, K;Dickerson, E;Kaufman, D;Modiano, J; | Transcriptional and Functional Activity of Hemangiosarcoma Support Bone Marrow Nurse Cell Ontogeny | bioRxiv | Human BM-derived mesenchymal stromal cells (hBM-MSCs) were isolated from whole bone marrow purchased from AllCells (Emeryville, CA, USA) as previously described [24-27]. M2- Save | Full Paper | |
2021 | Maughon, T;Shen, X;Huang, D;Michael, A;Shockey, W;Andrews, S;McRae, J;Platt, M;Fernández, F;Edison, A;Stice, S;Marklein, R; | Multi-omics characterization of mesenchymal stem/stromal cells for the identification of putative critical quality attributes | bioRxiv | PBMCs (AllCells, Alameda CA) were thawed in RPMI media (RPMI, 20% FBS, 2mM L-glutamine, 50 U/mL penicillin, 50 μg/mL streptomycin) and cultured overnight at 37°C and 5% CO2. Prior to co-culture, PBMCs were labeled with CFSE (Supplemental Table 1, Bio | Full Paper | |
2021 | Jain, S;Rick, J;Joshi, R;Beniwal, A;Spatz, J;Chang, A;Nguyen, A;Sudhir, S;Chandra, A;Haddad, A;Wadhwa, H;Shah, S;Choi, S;Hayes, J;Wang, L;Yagnik, G;Costello, J;Diaz, A;Aghi, M; | Identification of Cancer-Associated Fibroblasts in Glioblastoma and Defining Their Pro-tumoral Effects | bioRxiv | THP-1 cells and monocytes isolated from peripheral blood (AllCells) run through the MojoSort Human CD14 Selection Kit were treated with 50 ng/μL PMA (phorbol myristate acetate) Save | Full Paper | |
2021 | Andrews, S;Klinker, M;Bauer, S;Marklein, R; | Morphological Landscapes from High Content Imaging Identify Optimal Priming Strategies that Enhance MSC Immunosuppression | bioRxiv | Human bone marrow-derived MSCs were obtained from 4 different donors purchased from Lonza (Walkersville, MD, USA), AllCells (Emeryville, CA, USA), and RoosterBio (Frederick, MD, USA) (see Table S1 for donor information). MSC culture conditions for th | Full Paper | |
2021 | Gu, C;Cao, X;Wang, Z;Hu, X;Yao, Y;Zhou, Y;Liu, P;Liu, X;Gao, G;Hu, X;Zhang, Y;Chen, Z;Gao, L;Peng, Y;Jia, F;Shan, C;Yu, L;Liu, K;Li, N;Guo, W;Jiang, G;Min, J;Zhang, J;Yang, L;Shi, M;Hou, T;Li, Y;Liang, W;Lu, G;Yang, C;Wang, Y;Xia, K;Xiao, Z;Xue, J;Huang, X;Chen, X;Ma, H;Song, D;Pan, Z;Wang, X;Guo, H;Liang, H;Yuan, Z;Guan, W;Deng, S; | A human antibody with blocking activity to RBD proteins of multiple SARS-CoV-2 variants including B.1.351 showed potent prophylactic and therapeutic efficacy against SARS-CoV-2 in rhesus macaques | bioRxiv | Phage libraries were constructed using antibody gene fragments amplified from PBMCs of 50 healthy human subjects (Allcells, PB003F and PB003C) and total RNA from PBMCs ( Save | Full Paper | |
2021 | Fitzgerald, A;Marcisak, E;Nasir, A;Glasgow, E;Jablonski, S;Van Der Veken, P;Pearson, G;Fertig, E;Mace, E;Weiner, L; | Fibroblast activation protein regulates natural killer cell migration, extravasation and tumor infiltration | bioRxiv | from AllCells with either CD56 positive selection or CD56 negative selection (Allcells, cat# T cells, B cells and monocytes were isolated from PBMCs (Allcells) using Mojosort magnetic Save | Full Paper | |
2021 | Brooks, J; | Preclinical Dynamic Contrast Enhanced Imaging for Longitudinal Biophysical Assessments of the Healthy and Malignant Vasculature after Radiotherapy | Thesis | C57BL/6J mice (Strain 000664, Jackson Laboratory, Bar Harbor, ME) mice were injected with 1x106 GFP+ BCR-ABL (p190Kd) expressing B-cell ALLcells [66], suspended in 200 µl of phosphate buffered saline, through the tail vein. To test drug delivery, inj | Full Paper | |
2021 | O'Connor, EI; | Mechanisms Behind the Causal Role of Dietary Oxylipins of the Lipoxygenase Pathway in the Development of Pulmonary Arterial Hypertension | Thesis | PAECs were incubated with or without 15-HETE and 12 hours later healthy CD8 cells (ALLCELLS, Lot# 3003028) were added. After 12 hours of contact between EC and CD8 cells, the cells were collected, fixed, and stained for cleaved caspase 3 | Full Paper | |
2021 | McIntyre, P; | The Effects of a Pyk2 Kinase Inhibitor on the Proliferation and Differentiation of Human Dental Pulp Stem Cells | Thesis | Commercially available human DPSCs, collected from the pulp of extracted teeth, have been previously purchased from ALLCells and stored in liquid nitrogen (Alameda, CA, USA). • Save | Full Paper | |
2021 | Noaks, E; | Enhancing the consistency of donor derived peripheral blood mononuclear cells for CAR T-cell manufacturing | Thesis | For work completed at the company site healthy peripheral blood leukapheresis were obtained from the NHS or purchased as LeukoPaks from AllCells, Cellex and Anthony Nolan, as part of research study NREC ID 15/LO/1322. Experiments using healthy leukap | Full Paper | |
2021 | Osman, ZF;Zahari, W;Kannan, TP; | Ultrastructure changes in BMP-2-treated SHED cultured on glycerol preserved human amniotic membrane | Acta Microscópica | ommerciallysourcedSHED(AllCells,USA)wasculturedonde-epithelialized2cmx2cmglycerol-preserved γ-radiated AM in a culture dish containing 3 mlof media at different cell density for the respective days asthefollowing;2x105forday1,5x104forday7,2.5x104 | Full Paper | |
2021 | Luecken, MD;Burkhardt, DB;Cannoodt, R; | A sandbox for prediction and integration of dna, rna, and proteins in single cells | Conference | We sourced multiple samples of BMMCs from 10 donors via AllCells (California, USA), all healthy non-smokers without recent medical treatment. Donors varied by age (22 - 40), sex, Save | Full Paper | |
2021 | Vladimirova, T; | Implementation and Evaluation of Verlet List-based Methods in AutoPas | Thesis | Inside the 2D vector for AllCells or 3D vector for CellPair respectively, the method adds the particle indices corresponding to the particle pointers from the same spot in the AoS neighbor Save | Full Paper | |
2021 | Maser, IP; | In-depth characterization of myeloid enhanced humanized mouse strains | Thesis | was diluted with 0.9% sodium chloride (Braun) and 15 mg/kg was intraperitoneally (i.p.) injected into mice. 24 hours later 1×105 human CD34+ umbilical cord blood-derived hematopoietic stem cells (HSCs) (Stem Cell Technologies/Allcells) in 100 µl phos | Full Paper | |
2021 | Fitzgerald, AA; | Enhancing Anti-Cancer Properties of Human Natural Killer Cells | Thesis | Fresh healthy donor NK cells were purchased from AllCells with either CD56 positive selection or CD56 negative selection (Allcells, cat#PB012-P or PB012-N). T cells, B cells and monocytes were isolated from PBMCs (Allcells) using Mojosort magnetic ce | Full Paper | |
2021 | Butler, A; | Computational Methods for the Integration of Single-Cell Data | Thesis | We therefore repeated the original experiment, where PBMCs from a healthy human donor (ALLCELLS) were cultured with RPMI medium supplemented with 10% FBS and stimulated for 6 h with IFNβ (100 U/ml, PBL Assay Science), with a subset of cells left unex | Full Paper | |
2021 | Nataraja, S;Singh, M;Demes, S;Olson, L;Stanwix, J;Biddle, M;Clarke, E;Huang, Y;Nguyen, J;Chen, C;Zhang, P;Abdulla, F;Vercellotti, G;Belcher, J; | ML-0207/ASP8731: A Novel BACH1 Inhibitor That Induces Fetal Hemoglobin in Treatment of Sickle Cell Disease | Blood | Translational Development, Mitobridge, Cambridge, MA++Translational Science, Mitobridge, Cambridge, MA++Clinical Pharmacology and Exploratory Development, Astellas, Northbrook, IL++Translational Science, Mitobridge, Cambridge, MA++Translational Scien | Full Paper | |
2021 | Qing, Y;Dong, L;Gao, L;Li, C;Li, Y;Han, L;Prince, E;Tan, B;Deng, X;Wetzel, C;Shen, C;Gao, M;Chen, Z;Li, W;Zhang, B;Braas, D;Ten Hoeve, J;Sanchez, GJ;Chen, H;Chan, LN;Chen, CW;Ann, D;Jiang, L;Müschen, M;Marcucci, G;Plas, DR;Li, Z;Su, R;Chen, J; | R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axis | Molecular cell | 33434505 | The Lin− progenitor cells were then subject to retroviral transduction with MSCV-Neo-MA9 construct by two rounds of ‘spinoculation’, as described previously (Li et al., 2015). After being selected for 7 days with 0.5 mg/mL G418 Sulfate (10131027, Gib | Full Paper |
2021 | Li, C;Wang, H;Georgakopoulou, A;Gil, S;Yannaki, E;Lieber, A; | In Vivo HSC Gene Therapy Using a Bi-modular HDAd5/35++ Vector Cures Sickle Cell Disease in a Mouse Model | Molecular therapy : the journal of the American Society of Gene Therapy | 32949495 | The lineage-minus (Lin−) cells were isolated by depletion of lineage-committed cells in bone marrow MNCs using the Mouse Lineage Cell Depletion Kit (Miltenyi Biotec, San Diego, CA, USA) according to the manufacturer’s instructions. CFU assays were pe | Full Paper |
2021 | Loftus, JP;Yahiaoui, A;Brown, PA;Niswander, LM;Bagashev, A;Wang, M;Schauf, A;Tannheimer, S;Tasian, SK; | Combinatorial efficacy of entospletinib and chemotherapy in patient-derived xenograft models of infant acute lymphoblastic leukemia | Haematologica | 32414848 | s Supplementary Appendix Acknowledgments We acknowledge Dr Ann Forslund and Ms Chelsea Mullins formerly at Gilead Sciences for study protocol management and data analysis, and Dr Emer Clarke at ReachBio Research Labs for assistance with experim | Full Paper |
2021 | DiLillo, DJ;Olson, K;Mohrs, K;Meagher, TC;Bray, K;Sineshchekova, O;Startz, T;Kuhnert, J;Retter, MW;Godin, S;Sharma, P;Delfino, F;Lin, J;Smith, E;Thurston, G;Kirshner, JR; | A BCMAxCD3 bispecific T cell-engaging antibody demonstrates robust antitumor efficacy similar to that of anti-BCMA CAR T cells | Blood advances | 33651100 | T cells were purified from normal donor PBMCs (ReachBio) by using magnetic beads (Thermo Fisher Scientific) and activated with CD3/CD28 microbeads (Thermo Fisher Scientific) in Save | Full Paper |
2021 | Byrgazov, K;Besse, A;Kraus, M;Slipicevic, A;Lehmann, F;Driessen, C;Besse, L; | Novel Peptide-drug Conjugate Melflufen Efficiently Eradicates Bortezomib-resistant Multiple Myeloma Cells Including Tumor-initiating Myeloma Progenitor Cells | HemaSphere | 34136753 | of MM were assessed in a semisolid methylcellulose-based media formulation containing 10% phytohemagglutinin (PHA)-stimulated leukocyte conditioned medium (ReachBio, WA). Save;cell outgrowth assay Clonogenic progenitors of MM were assessed in a sem | Full Paper |
2021 | Swaminathan, M;Kopyt, N;Atta, MG;Radhakrishnan, J;Umanath, K;Nguyen, S;O'Rourke, B;Allen, A;Vaninov, N;Tilles, A;LaPointe, E;Blair, A;Gemmiti, C;Miller, B;Parekkadan, B;Barcia, RN; | Pharmacological effects of ex vivo mesenchymal stem cell immunotherapy in patients with acute kidney injury and underlying systemic inflammation | Stem cells translational medicine | 34581517 | Flow cytometry testing of patient leukocytes was performed by ReachBio (Spokane, Washington). Blood was collected in CPT tubes (362 753; BD Biosciences), and peripheral blood Save;n a plasma preparation tube (362 788; BD Biosciences, San Jose, Cali | Full Paper |
2021 | Lin, M;Kowolik, CM;Xie, J;Yadav, S;Overman, LE;Horne, DA; | Potent Anticancer Effects of Epidithiodiketopiperazine NT1721 in Cutaneous T Cell Lymphoma | Cancers | 34282785 | TCC (Manassas, VA, USA), authenticated by STR-profiling at the source and passaged for less than 6 months after receipt or resuscitation. Peripheral blood mononuclear cells (PBMCs) were obtained from ReachBio (Spokane, WA, USA). All cells were grown | Full Paper |
2021 | Samuelson, C;Radtke, S;Zhu, H;Llewellyn, M;Fields, E;Cook, S;Huang, MW;Jerome, KR;Kiem, HP;Humbert, O; | Multiplex CRISPR/Cas9 genome editing in hematopoietic stem cells for fetal hemoglobin reinduction generates chromosomal translocations | Molecular therapy. Methods & clinical development | 34853798 | cation event frequencies was also undertaken on each of these tissues from necropsy as per protocol above. CFU and HbF assays on mouse BM A total of 7 × 104/mL cells were plated in ColonyGEL 1402 (ReachBio, Seattle, WA) in triplicate from each mouse | Full Paper |
2021 | Sharifi, S;Sharifi, H;Akbari, A;Chodosh, J; | Systematic optimization of visible light-induced crosslinking conditions of gelatin methacryloyl (GelMA) | Scientific reports | 34857867 | and exposed to photoirradiation for a 4 and 10 min. Those cell-laden gels were immediately washed 3 times with PBS and incubated in 200 μL of an optimized serum-containing medium (catalog # 02,100, Reachbio lab, Spokane, WA) at 37 °C. Cell viability | Full Paper |
2021 | Haber, L;Olson, K;Kelly, MP;Crawford, A;DiLillo, DJ;Tavaré, R;Ullman, E;Mao, S;Canova, L;Sineshchekova, O;Finney, J;Pawashe, A;Patel, S;McKay, R;Rizvi, S;Damko, E;Chiu, D;Vazzana, K;Ram, P;Mohrs, K;D'Orvilliers, A;Xiao, J;Makonnen, S;Hickey, C;Arnold, C;Giurleo, J;Chen, YP;Thwaites, C;Dudgeon, D;Bray, K;Rafique, A;Huang, T;Delfino, F;Hermann, A;Kirshner, JR;Retter, MW;Babb, R;MacDonald, D;Chen, G;Olson, WC;Thurston, G;Davis, S;Lin, JC;Smith, E; | Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuning | Scientific reports | 34257348 | ce with the ARRIVE guidelines. OVCAR-3 bioluminescence imaging (BLI) study Eight-week-old NOD SCID gamma chain knock-out (NSG) mice (Jackson Laboratory) were injected with 5 × 106 human PBMCs (ReachBio) before 1 × 106 ascites cells from the OVCAR-3/ | Full Paper |
2021 | Patterson, AM;Sellamuthu, R;Plett, PA;Sampson, CH;Chua, HL;Fisher, A;Vemula, S;Feng, H;Katz, BP;Tudor, G;Miller, SJ;MacVittie, TJ;Booth, C;Orschell, CM; | Establishing Pediatric Mouse Models of the Hematopoietic Acute Radiation Syndrome and the Delayed Effects of Acute Radiation Exposure | Radiation research | 33577641 | elsewhere (47). Hematopoietic progenitor cell (HPC) number and function were quantitated from 1.0 × 10e5 low-density bone marrow (LDBM) in 1 ml ColonyGEL™ Mouse Complete Medium (ReachBio Research Labs, Seattle, WA) | Full Paper |
2021 | Rethnam, M;Tan, DQ;Suda, T; | Myeloma cells self-promote migration by regulating TAB1-driven TIMP-1 expression in mesenchymal stem cells | Biochemical and biophysical research communications | 33183761 | Human BM MSCs (#02100, ReachBio) were grown in DMEM low glucose media (Biowest) supplemented with 10% FBS and 1% Antibiotic/Anti-mycotic (Gibco). Cells were passaged upon confluence and cultured at a density of 4.5 × 103 cells/cm2. U266-B1 (TIB-196, | Full Paper |
2021 | Andersen, A;Vieira-Brock, PL;Vaughan, B;Vollmer, D; | Method development for the analysis of PBMC-mediated killing of K562 cells by bovine colostrum | Journal of immunological methods | 34744024 | Declaration of Competing Interest Authors and principle investigators are employed by 4Life Research, which sells the test articles commercially. 4Life Research funded all portions of this study. Acknowledgements All testing was performed at ReachBi | Full Paper |
2021 | Radtke, S;Enstrom, M;Pande, D;Cui, M;Madhu, R;Perez, A;Kiem, H; | Hematopoietic recovery after transplantation is primarily derived from the stochastic contribution of hematopoietic stem cells | bioRxiv | For CFC assays, 800-1,200 sort-purified CD34+ cells and CD34-subpopulations were seeded into 3.5 ml ColonyGEL 1402 (ReachBio, Seattle, WA). Colonies were scored after 12 to 14 days, discriminating colony forming unit- (CFU-) granulocyte (CFU-G), macr | Full Paper | |
2020 | Geoerger, B;Kang, HJ;Yalon-Oren, M;Marshall, LV;Vezina, C;Pappo, A;Laetsch, TW;Petrilli, AS;Ebinger, M;Toporski, J;Glade-Bender, J;Nicholls, W;Fox, E;DuBois, SG;Macy, ME;Cohn, SL;Pathiraja, K;Diede, SJ;Ebbinghaus, S;Pinto, N; | Pembrolizumab in paediatric patients with advanced melanoma or a PD-L1-positive, advanced, relapsed, or refractory solid tumour or lymphoma (KEYNOTE-051): interim analysis of an open-label, single-arm, phase 1–2 trial | Lancet Oncol. | 31812554 | In all patients, baseline PD-L1 expression in tumour samples (positivity defined as any staining of the stroma or PD-L1 expression on at least 1% of tumour cells) was assessed centrally at QualTek Molecular Laboratories (Goleta, CA, USA) using a prot | Full Paper |
2020 | Emens, LA;Esteva, FJ;Beresford, M;Saura, C;De Laurentiis, M;Kim, SB;Im, SA;Wang, Y;Salgado, R;Mani, A;Shah, J;Lambertini, C;Liu, H;de Haas, SL;Patre, M;Loi, S; | Trastuzumab emtansine plus atezolizumab versus trastuzumab emtansine plus placebo in previously treated, HER2-positive advanced breast cancer (KATE2): a phase 2, multicentre, randomised, double-blind trial | The Lancet. Oncology | 33002436 | The KATE2 study is a randomised, double-blind, placebo-controlled, phase 2 trial at 68 centres from nine countries across Asia, Australia, North America, and western Europe (appendix pp 13–15). Eligible patients were adults (aged ≥18 years) with cent | Full Paper |
2020 | Powell, SF;Gold, KA;Gitau, MM;Sumey, CJ;Lohr, MM;McGraw, SC;Nowak, RK;Jensen, AW;Blanchard, MJ;Fischer, CD;Bykowski, J;Ellison, CA;Black, LJ;Thompson, PA;Callejas-Valera, JL;Lee, JH;Cohen, EEW;Spanos, WC; | Safety and Efficacy of Pembrolizumab With Chemoradiotherapy in Locally Advanced Head and Neck Squamous Cell Carcinoma: A Phase IB Study | J. Clin. Oncol. | 32479189 | Testing was performed centrally by QualTek Laboratories (Santa Barbara, CA) using the PD-L1 IHC 22C3 pharmDx (Dako North America, Carpinteria, CA) IHC assay as previously described. 25 PD-L1 evaluation was performed using the combined proportional sc | Full Paper |
2020 | Marabelle, A;Cassier, P;Fakih, M;Kao, S;Nielsen, D;Italiano, A;Guren, T;Dongen, M;Spencer, K;Bariani, G;Ascierto, P;Santoro, A;Hiret, S;Ott, P;Piha-Paul, S;Liu, C;Leiby, M;Norwood, K;Delord, J; | Pembrolizumab for previously treated advanced anal squamous cell carcinoma: Pooled results from the KEYNOTE-028 and KEYNOTE-158 studies. | JCO | PD-L1 positivity). Baseline PD-L1 expression was assessed using a prototype IHC assay (QualTek) in KEYNOTE-028 and the PD-L1 IHC 22C3 pharmDx assay (Agilent Technologies) in KEYNOTE-158. Pts received pembro | Full Paper | |
2020 | Greenstein, A;Munster, P;Sachdev, J;Fleming, G;Grauer, A;Shepherd, S; | Impact of relacorilant, a selective glucocorticoid receptor antagonist, on the immunosuppressive effects of endogenous cortisol. | JCO | Tina K. Schlafly of Corcept Therapeutics. Funding for editorial, design, and production support for this poster was provided by Corcept to MedVal Scientific Information Services, LLC, Princeton, NJ. Experiments were conducted at Ardigen SA, QualTek M | Full Paper | |
2020 | Dudek, AZ;Liu, LC;Gupta, S;Logan, TF;Singer, EA;Joshi, M;Zakharia, YN;Lang, JM;Schwarz, JK;Al-Janadi, A;Alva, AS; | Phase Ib/II Clinical Trial of Pembrolizumab With Bevacizumab for Metastatic Renal Cell Carcinoma: BTCRC-GU14-003 | J. Clin. Oncol. | 32097091 | Events (CTCAE; version 4.0). Exploratory Studies. Expression of PD-L1 in archived diagnostic tumor tissue was determined by immunohistochemistry (IHC) using a 22C3 antibody (Qualtek Electronics, Newton, PA). Both a modified ;logy Criteria for Ad | Full Paper |
2020 | Tolaney, SM;Barroso-Sousa, R;Keenan, T;Li, T;Trippa, L;Vaz-Luis, I;Wulf, G;Spring, L;Sinclair, NF;Andrews, C;Pittenger, J;Richardson, ET;Dillon, D;Lin, NU;Overmoyer, B;Partridge, AH;Van Allen, E;Mittendorf, EA;Winer, EP;Krop, IE; | Effect of Eribulin With or Without Pembrolizumab on Progression-Free Survival for Patients With Hormone Receptor-Positive, ERBB2-Negative Metastatic Breast Cancer: A Randomized Clinical Trial | JAMA oncology | 32880602 | A total of 65 patients (74%) had PD-L1 testing assessed centrally by QualTek using the 22C3 antibody, and results were reported as a modified pro- portion score (MPS), defined as the proportion of cells, includ- ing both tumor and mononuclear inflamm | Full Paper |
2020 | Zsiros, E;Lynam, S;Attwood, KM;Wang, C;Chilakapati, S;Gomez, EC;Liu, S;Akers, S;Lele, S;Frederick, PJ;Odunsi, K; | Efficacy and Safety of Pembrolizumab in Combination With Bevacizumab and Oral Metronomic Cyclophosphamide in the Treatment of Recurrent Ovarian Cancer: A Phase 2 Nonrandomized Clinical Trial | JAMA oncology | 33211063 | A modified proportion score threshold of at least 1% using the 22C3 antibody (QualTekMolecular Laboratories) was adopted similarly to previously described protocols.6 | Full Paper |
2020 | Jabbour, SK;Berman, AT;Decker, RH;Lin, Y;Feigenberg, SJ;Gettinger, SN;Aggarwal, C;Langer, CJ;Simone, CB;Bradley, JD;Aisner, J;Malhotra, J; | Phase 1 Trial of Pembrolizumab Administered Concurrently With Chemoradiotherapy for Locally Advanced Non-Small Cell Lung Cancer: A Nonrandomized Controlled Trial | JAMA Oncol | 32077891 | Exploratory analyses included PD-L1 status, by mean proportion score. Immunohistochemical staining for PD-L1 used a mouse monoclonal anti-PD-L1 antibody (clone 22C3).21 Furthermore, TILs were scored on a continuum from 0 to 3 (0 indicates absent TILs | Full Paper |
2020 | Kelly, CM;Antonescu, CR;Bowler, T;Munhoz, R;Chi, P;Dickson, MA;Gounder, MM;Keohan, ML;Movva, S;Dholakia, R;Ahmad, H;Biniakewitz, M;Condy, M;Phelan, H;Callahan, M;Wong, P;Singer, S;Ariyan, C;Bartlett, EK;Crago, A;Yoon, S;Hwang, S;Erinjeri, JP;Qin, LX;Tap, WD;D'Angelo, SP; | Objective Response Rate Among Patients With Locally Advanced or Metastatic Sarcoma Treated With Talimogene Laherparepvec in Combination With Pembrolizumab: A Phase 2 Clinical Trial | JAMA Oncol | 31971541 | Paraffin-embedded tumor material taken before and after treatment was examined for TIL immune biomarker expression, including PD-L1, PD-1, CD3, CD4, FOXP3, CD8, CD68, CD163, and MHC1 by Qualtek Laboratory. Programmed death-ligand 1 tumor membranous e | Full Paper |
2020 | Mancuso, F;Ogbah, Z;Fasani, R;Lo Giacco, D;Ramos, L;Oaknin, A;Alsina, M;Elez, E;Mercade, T;Saura, C;Capdevila, J;Vieito, M;Felip, E;Dienstmann, R;Gros, A;Seoane, J;Nuciforo, P;Tabernero, J;Garralda, E;Vivancos, A; | 581P VHIO immune gene expression profiling (VIGex) panel, a tool to explore tumour immune microenvironment | Annals of Oncology | self), Speaker Bureau/Expert testimony, Research grant/Funding (self), Travel/Accommodation/ Expenses: Novartis; Honoraria (self), Speaker Bureau/Expert testimony, Research grant/Funding (self), Travel/Accommodation/Expenses: Targos Molecular Patholo | Full Paper | |
2020 | Jotte, R;Cappuzzo, F;Vynnychenko, I;Stroyakovskiy, D;Rodríguez-Abreu, D;Hussein, M;Soo, R;Conter, HJ;Kozuki, T;Huang, KC;Graupner, V;Sun, SW;Hoang, T;Jessop, H;McCleland, M;Ballinger, M;Sandler, A;Socinski, MA; | Atezolizumab in Combination With Carboplatin and Nab-Paclitaxel in Advanced Squamous NSCLC (IMpower131): Results From a Randomized Phase III Trial | J Thorac Oncol | 32302702 | Skip to Main Content ;This work was supported by F. Hoffmann-La Roche Ltd/Genentech, Inc., a member of the Roche Group. The authors would like to acknowledge Targos Molecular Pathology GmbH (Kassel, Germany) for performing central PD-L1 and T-ef | Full Paper |
2020 | M.C.Liu, G.R.Oxnard, E.A.Klein, C.Swanton, M.V.Seiden | Sensitive and specific multi-cancer detection and localization using methylation signatures in cell-free DNA | Annals of Oncology | Background: Early cancer detection could identify tumors at a time when outcomes are superior and treatment is less morbid. This prospective case-control sub-study (from NCT02889978 and NCT03085888) assessed the performance of targeted methylation an | Full Paper | |
2020 | Taylor, WC; | Comment on "Sensitive and specific multi-cancer detection and localization using methylation signatures in cell-free DNA"" by M. C. Liu et al" | Ann. Oncol. | 32371122 | cfDNA [n = 2628 (1493 cancer; 1135 non-cancer)], formalin fixed paraffin embedded (FFPE) tumor biopsies (n = 242), and WBCs (n = 70) from the first CCGA sub-study; commercial tissue or cells [n = 227; Discovery Life Sciences (formerly Conversant Biol | Full Paper |
2020 | Draz, MS;Vasan, A;Muthupandian, A;Kanakasabapathy, MK;Thirumalaraju, P;Sreeram, A;Krishnakumar, S;Yogesh, V;Lin, W;Yu, XG;Chung, RT;Shafiee, H; | Virus detection using nanoparticles and deep neural network-enabled smartphone system | Science advances | 33328239 | Deidentified HCV-infected and HBV-infected patient plasma/ serum samples were obtained from Discovery Life Sciences Inc | Full Paper |
2020 | Chung, HC;Piha-Paul, SA;Lopez-Martin, J;Schellens, JHM;Kao, S;Miller, WH;Delord, JP;Gao, B;Planchard, D;Gottfried, M;Zer, A;Jalal, SI;Penel, N;Mehnert, JM;Matos, I;Bennouna, J;Kim, DW;Xu, L;Krishnan, S;Norwood, K;Ott, PA; | Pembrolizumab After Two or More Lines of Previous Therapy in Patients With Recurrent or Metastatic SCLC: Results From the KEYNOTE-028 and KEYNOTE-158 Studies | J Thorac Oncol | 31870883 | In KEYNOTE-028, tumor samples were assessed using a laboratory-developed prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA) with the 22C3 antibody clone (Merck Sharp & Dohme Corp., a subsidiary of Merck & Co., Inc., Ken | Full Paper |
2020 | Freeman, ZT;Nirschl, TR;Hovelson, DH;Johnston, RJ;Engelhardt, JJ;Selby, MJ;Kochel, CM;Lan, RY;Zhai, J;Ghasemzadeh, A;Gupta, A;Skaist, AM;Wheelan, SJ;Jiang, H;Pearson, AT;Snyder, LA;Korman, AJ;Tomlins, SA;Yegnasubramanian, S;Drake, CG; | A conserved intratumoral regulatory T cell signature identifies 4-1BB as a pan-cancer target | J. Clin. Invest. | 32015231 | Study approval. All human tumors were collected with approval from the Johns Hopkins University IRB with the following protocol IDs: renal clear cell carcinoma (IRB00033839), prostate cancer (NA_00082175), bladder cancer (NA_00026693), and glioblasto | Full Paper |
2020 | Weiss, J;Sheth, S;Deal, AM;Grilley Olson, JE;Patel, S;Hackman, TG;Blumberg, JM;Galloway, T;Patel, S;Zanation, AM;Shen, CJ;Hayes, DN;Hilliard, C;Mehra, R;McKinnon, KP;Wang, HH;Weissler, MC;Bauman, JR;Chera, BS;Vincent, BG; | Concurrent definitive immunoradiotherapy for patients with Stage III-IV Head and Neck Cancer and Cisplatin contraindication | Clin. Cancer Res. | 32371539 | withdrawal of permission. Correlative Analysis PDL1 staining was conducted by Qualtek Molecular Laboratories (Newtown, PA) as previously described 5 and required by Merck in supported investigator initiated trials. For both | Full Paper |
2020 | Graff, JN;Beer, TM;Alumkal, JJ;Slottke, RE;Redmond, WL;Thomas, GV;Thompson, RF;Wood, MA;Koguchi, Y;Chen, Y;Latour, E;Bergan, RC;Drake, CG;Moran, AE; | A phase II single-arm study of pembrolizumab with enzalutamide in men with metastatic castration-resistant prostate cancer progressing on enzalutamide alone | J Immunother Cancer | 32616555 | retained. Formalin-fixed, paraffin-embedded tumor samples were sent to a commercial vendor (Qualtek) for PD-L1 quantification by immunohistochemistry (clone 22C3). Additional methods details are described elsewhere.30 | Full Paper |
2020 | Majd, N;Waguespack, S;Janku, F;Fu, S;Penas-Prado, M;Xu, M;Alshawa, A;Kamiya-Matsuoka, C;Raza, S;McCutcheon, I;Naing, A; | Efficacy of pembrolizumab in patients with pituitary carcinoma: report of four cases from a phase II study | Journal for ImmunoTherapy of Cancer | PD-L1 staining was performed by Qualtek using Merck 22C3 antibody for PD-L1 | Full Paper | |
2020 | Lokeshwar, VB;Morera, DS;Hasanali, SL;Yates, TJ;Hupe, MC;Knapp, J;Lokeshwar, SD;Wang, J;Hennig, MJP;Baskar, R;Escudero, DO;Racine, RR;Dhir, N;Jordan, AR;Hoye, K;Azih, I;Manoharan, M;Klaassen, Z;Kavuri, S;Lopez, LE;Ghosh, S;Lokeshwar, BL; | A Novel Splice Variant of HYAL-4 Drives Malignant Transformation and Predicts Outcome in Patients with Bladder Cancer | Clin. Cancer Res. | 32094233 | Present address: Travis Yates: QualTek Molecular Laboratories, King of Prussia, PA; Diogo Escudero: George Washington University Kelly Hoye: University of North Carolina Lineberger Comprehensive Cancer Center; Ronny Racine: Astellas Institute for Reg | Full Paper |
2020 | Fong, LY;Taccioli, C;Palamarchuk, A;Tagliazucchi, GM;Jing, R;Smalley, KJ;Fan, S;Altemus, J;Fiehn, O;Huebner, K;Farber, JL;Croce, CM; | Abrogation of esophageal carcinoma development in miR-31 knockout rats | Proc. Natl. Acad. Sci. U.S.A. | 32123074 | lia isolated using the DNeasy Blood & Tissue Kit (Qiagen). WGS. DNA library preparation, library quality control, and nonhuman WGS to yield ∼30× coverage were performed on the Illumina HiSeq X at HudsonAlpha Genomic Services Laboratory. WGS Analysi | Full Paper |
2020 | Rodriguez, CP;Wu, QV;Voutsinas, J;Fromm, JR;Jiang, X;Pillarisetty, VG;Lee, SM;Santana-Davila, R;Goulart, B;Baik, CS;Chow, LQM;Eaton, K;Martins, R; | A Phase II Trial of Pembrolizumab and Vorinostat in Recurrent Metastatic Head and Neck Squamous Cell Carcinomas and Salivary Gland Cancer | Clin. Cancer Res. | 31796519 | Submission of archived tissue was required prior to study entry. In patient with no available archived tissue, fresh tissue biopsies were recommended. Biopsies were attempted after three cycles of therapy were completed, in patients who consented. PD | Full Paper |
2020 | Huetter, J;Gritzan, U;Gutcher, I;Doecke, WD;Luetke-Eversloh, MV;Golfier, S;Roider, HG;Frisk, AL;Hunter, J;Pow, A;Drake, A;Levine, Z;Levy, O;Azulay, M;Barbiro, I;Cojocaru, G;Vaknin, I;Kreft, B;Roese, L; | Characterization of BAY 1905254, an immune checkpoint inhibitor targeting the immunoglobulin-like domain containing receptor 2 (ILDR2) | Cancer Immunol Res | 32312711 | cancer patients, and commercially available cryopreserved human lymph node single- cell preparations from melanoma patients (Folio Conversant, USA) using the PrimeFlow™ RNA Assay technology (Invitrogen, CA, USA). Following enzymatic | Full Paper |
2020 | Ozkan-Dagliyan, I;Diehl, JN;George, SD;Schaefer, A;Papke, B;Klotz-Noack, K;Waters, AM;Goodwin, CM;Gautam, P;Pierobon, M;Peng, S;Gilbert, TSK;Lin, KH;Dagliyan, O;Wennerberg, K;Petricoin, EF;Tran, NL;Bhagwat, SV;Tiu, RV;Peng, SB;Herring, LE;Graves, LM;Sers, C;Wood, KC;Cox, AD;Der, CJ; | Low-Dose Vertical Inhibition of the RAF-MEK-ERK Cascade Causes Apoptotic Death of KRAS Mutant Cancers | Cell Rep | 32553168 | CRISPR sequencing was performed at UNC (High-Throughput Sequencing Facility, Chapel Hill, NC) and Hudson Alpha (HudsonAlpha Genomic Services Laboratory, Huntsville, AL) | Full Paper |
2020 | Wang, X;Kelkar, YD;Xiong, X;Martinson, EO;Lynch, J;Zhang, C;Werren, JH;Wang, X; | Genome Report: Whole Genome Sequence and Annotation of the Parasitoid Jewel Wasp Nasonia giraulti Laboratory Strain RV2X[u] | G3 (Bethesda) | 32571804 | and Helen Wisch Professorship. X.X. is supported by the Auburn University Presidential Graduate Research Fellowship and Auburn University College of Veterinary Medicine Dean’s Fellowship. We thank HudsonAlpha Discovery for assistance with Illumina se | Full Paper |
2020 | Ulasov, I;Borovjagin, A;Fares, J;Yakushov, S;Malin, D;Timashev, P;Lesniak, MS; | MicroRNA 345 (miR345) regulates KISS1-E-cadherin functional interaction in breast cancer brain metastases | Cancer Lett. | 32246957 | One hundred and eleven tumor samples, representing invasive ductal carcinoma (IDC) were subjected for evaluation as a part of brain tissue microarrays presented by: US Biomax (BR812), Folio Biosciences (AY-HH0137) and biobank at the University of Chi | Full Paper |
2020 | Chiang, J;Ahmed, N;Foote, J;Prasad, N;Karri, S;Pagidas, K;Wiltshire, A;Gunn, D;Singh, K; | OVARIAN AGING AND REPRODUCTIVE SENESCENCE IN MOUSE MODEL OF MITOCHONDRIAL DYSFUNCTION | Fertility and Sterility | 2 HudsonAlpha Discovery, Huntsville, AL; 3 TCM University, Lincoln, RI | Full Paper | |
2020 | Ho, AY;Barker, CA;Arnold, BB;Powell, SN;Hu, ZI;Gucalp, A;Lebron-Zapata, L;Wen, HY;Kallman, C;D'Agnolo, A;Zhang, Z;Flynn, J;Dunn, SA;McArthur, HL; | A phase 2 clinical trial assessing the efficacy and safety of pembrolizumab and radiotherapy in patients with metastatic triple-negative breast cancer | Cancer | 31747077 | PD‐L1 tumor expression was evaluated in prospectively collected samples of metastatic biopsies by a central laboratory (QualTek Molecular Laboratories, Goleta, California) using a previously described prototype assay using the 22C3 antibody (Merck an | Full Paper |
2020 | Kasimir-Bauer, S;Roder, J;Obermayr, E;Mahner, S;Vergote, I;Loverix, L;Braicu, E;Sehouli, J;Concin, N;Kimmig, R;Net, L;Roder, H;Zeillinger, R;Aust, S;On Behalf Of The Ovcad Ovarian Cancer Diagnosis Initiative, ; | Definition and Independent Validation of a Proteomic-Classifier in Ovarian Cancer | Cancers | 32899818 | These samples were obtained from commercial biobanks (Conversant Bio (Huntsville, AL, USA) and Oncology Metrics (Forth Worth, TX, USA)), under their ethics-approved protocols | Full Paper |
2020 | Jimenez, C;Subbiah, V;Stephen, B;Ma, J;Milton, D;Xu, M;Zarifa, A;Akhmedzhanov, FO;Tsimberidou, A;Habra, MA;Rodon Anhert, J;Fu, S;Naing, A; | Phase II Clinical Trial of Pembrolizumab in Patients with Progressive Metastatic Pheochromocytomas and Paragangliomas | Cancers | 32824391 | tissue samples or newly obtained biopsy specimens (if archival tissue was not available) were evaluated by immunohistochemical analysis to determine the expression of PDL-1 in tumor cells and tumor-infiltrating mononuclear inflammatory cells by Qualt | Full Paper |
2020 | Jordan, AR;Wang, J;Yates, TJ;Hasanali, SL;Lokeshwar, SD;Morera, DS;Shamaladevi, N;Li, CS;Klaassen, Z;Terris, MK;Thangaraju, M;Singh, AB;Soloway, MS;Lokeshwar, VB; | Molecular targeting of renal cell carcinoma by an oral combination | Oncogenesis | 32427869 | Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, 1410 Laney Walker Blvd., Augusta, GA, 30912, USA.||Sheila and David Fuente Graduate Program in Cancer Biology, University of Miami-Miller School of Medi | Full Paper |
2020 | Mehnert, JM;Bergsland, E;O'Neil, BH;Santoro, A;Schellens, JHM;Cohen, RB;Doi, T;Ott, PA;Pishvaian, MJ;Puzanov, I;Aung, KL;Hsu, C;Le Tourneau, C;Hollebecque, A;Élez, E;Tamura, K;Gould, M;Yang, P;Stein, K;Piha-Paul, SA; | Pembrolizumab for the treatment of programmed death-ligand 1-positive advanced carcinoid or pancreatic neuroendocrine tumors: Results from the KEYNOTE-028 study | Cancer | 32320048 | PD was confirmed by repeat radiographic imaging ≥4 weeks after the initial scan. Investigators could opt to keep clinically stable patients on treatment until confirmed PD. Safety was monitored through 30 days after the last treatment dose (90 days f | Full Paper |
2020 | Wen, X;Ou, Y;Zarick, H;Zhang, X;Hmelo, A;Victor, Q;Paul, E;Slocik, J;Naik, R;Bellan, L;Lin, E;Bardhan, R; | PRADA: Portable Reusable Accurate Diagnostics with nanostar Antennas for multiplexed biomarker screening | Bioeng Transl Med | labor intensive, and cost prohibitive.39,40 We chose to measure the accuracy of PRADA in commercially available de-identified human patient serum (Discovery Life Sciences Inc.) to recapitulate clinical diagnostics where biomarkers of interest compete | Full Paper | |
2020 | Gonzalez-Ericsson, PI;Stovgaard, ES;Sua, LF;Reisenbichler, E;Kos, Z;Carter, JM;Michiels, S;Le Quesne, J;Nielsen, TO;Laenkholm, AV;Fox, SB;Adam, J;Bartlett, JM;Rimm, DL;Quinn, C;Peeters, D;Dieci, MV;Vincent-Salomon, A;Cree, I;Hida, AI;Balko, JM;Haynes, HR;Frahm, I;Acosta-Haab, G;Balancin, M;Bellolio, E;Yang, W;Kirtani, P;Sugie, T;Ehinger, A;Castaneda, CA;Kok, M;McArthur, H;Siziopikou, K;Badve, S;Fineberg, S;Gown, A;Viale, G;Schnitt, SJ;Pruneri, G;Penault-Llorca, F;Hewitt, S;Thompson, EA;Allison, KH;Symmans, WF;Bellizzi, AM;Brogi, E;Moore, DA;Larsimont, D;Dillon, DA;Lazar, A;Lien, H;Goetz, MP;Broeckx, G;El Bairi, K;Harbeck, N;Cimino-Mathews, A;Sotiriou, C;Adams, S;Liu, SW;Loibl, S;Chen, IC;Lakhani, SR;Juco, JW;Denkert, C;Blackley, EF;Demaria, S;Leon-Ferre, R;Gluz, O;Zardavas, D;Emancipator, K;Ely, S;Loi, S;Salgado, R;Sanders, M;, ; | The path to a better biomarker: application of a risk management framework for the implementation of PD-L1 and TILs as immuno-oncology biomarkers in breast cancer clinical trials and daily practice | J. Pathol. | 32129476 | PANACEA NCT02129556 58 Pembrolizumab + trastuzumab single arm phase Ib/II PreTx LAdv or mHER2+ BC Ib: PD‐L1+ (6) II: PD‐L1+ & PD‐L1‐ (52) PD‐L1 (QualTek/ 22C3) tested prospectively at BTx (58). TILs were evaluated retrospectively (48). II: H | Full Paper |
2020 | Lu, H;Molony, RD;Chen, D;Jang, S;Wolf, B;Ewert, S;Flaherty, M;Xu, F;Isim, S;Shim, Y;Dornelas, C;Balke, N;Leber, XC;Scharenberg, M;Koelln, J;Choi, E;Ward, R;Johnson, J;Calzascia, T;Isnardi, I;Williams, JA;Lindenbergh, PL;van de Donk, NWCJ;Mutis, T;Huet, H;Lees, E;Meyer, MJ; | Development of anti-CD32b antibodies with enhanced Fc function for the treatment of B and plasma cell malignancies | Mol. Cancer Ther. | 32847974 | detect CD32b expression on primary cells and cell lines. Patient samples for CD32b expression analysis were purchased from Conversant Bio, now part of Discovery Life Science. 2B6 binding to normal human plasma cells from | Full Paper |
2020 | Hutchins, B;Starling, GC;McCoy, MA;Herzyk, D;Poulet, FM;Dulos, J;Liu, L;Kang, SP;Fayadat-Dilman, L;Hsieh, M;Andrews, CL;Ayanoglu, G;Cullen, C;Malefyt, RW;Kastelein, RA;Saux, SL;Lee, J;Li, S;Malashock, D;Sadekova, S;Soder, G;van Eenennaam, H;Willingham, A;Yu, Y;Streuli, M;Carven, GJ;van Elsas, A; | Biophysical and Immunological Characterization and In Vivo Pharmacokinetics and Toxicology in Nonhuman Primates of the Anti-PD-1 Antibody Pembrolizumab | Molecular cancer therapeutics | 32229606 | See Supplementary Data for additional details. Primary tumor cell culture assay Fresh non-small cell lung cancer (NSCLC) tumor tissues were collected and shipped overnight in AQIX media (AQIX) by collection providers (Folio Biosciences, Bio-Options, | Full Paper |
2020 | Smith, SD;Till, BG;Shadman, MS;Lynch, RC;Cowan, AJ;Wu, QV;Voutsinas, J;Rasmussen, HA;Blue, K;Ujjani, CS;Shustov, A;Cassaday, RD;Fromm, JR;Gopal, AK; | Pembrolizumab with R-CHOP in previously untreated diffuse large B-cell lymphoma: potential for biomarker driven therapy | Br. J. Haematol. | 32030732 | PD‐L1 immunohistochemistry was performed centrally by QualTek Molecular Laboratories using the mouse monoclonal antibody clone 22c3 by previously described methods (Supplementary Appendix, Section B) (Dolled‐Filhart et al. , 2016). Statistical consid | Full Paper |
2020 | Vijayvergia, N;Dasari, A;Deng, M;Litwin, S;Al-Toubah, T;Alpaugh, RK;Dotan, E;Hall, MJ;Ross, NM;Runyen, MM;Denlinger, CS;Halperin, DM;Cohen, SJ;Engstrom, PF;Strosberg, JR; | Pembrolizumab monotherapy in patients with previously treated metastatic high-grade neuroendocrine neoplasms: joint analysis of two prospective, non-randomised trials | Br. J. Cancer | 32152503 | For patients enrolled into FC, PD-L1 immunohistochemistry (IHC) assay was performed at Qualtek Research Laboratories on formalin-fixed, paraffin-embedded tissue sections using anti-PD-L1 monoclonal antibody clone 22C3 [Merck Research Laboratories] | Full Paper |
2020 | Schruf, E;Schroeder, V;Le, HQ;Schönberger, T;Raedel, D;Stewart, EL;Fundel-Clemens, K;Bluhmki, T;Weigle, S;Schuler, M;Thomas, MJ;Heilker, R;Webster, MJ;Dass, M;Frick, M;Stierstorfer, B;Quast, K;Garnett, JP; | Recapitulating idiopathic pulmonary fibrosis related alveolar epithelial dysfunction in a human iPSC-derived air-liquid interface model | FASEB J. | 32297676 | Formalin-fixed and paraffin embedded lung samples from human IPF patients were purchased from Folio Biosciences (Powell, OH, US) under the regulatory conditions of the Boehringer Ingelheim corporate policy regarding the acquisition and use of human b | Full Paper |
2020 | Kang, J;Yeom, G;Jang, H;Park, C;Kim, M; | Highly sensitive and universal detection strategy based on a colorimetric assay using target-specific heterogeneous sandwich DNA aptamer | Analytica Chimica Acta | All reagent concentrations were the same as those analyzed in the developed method. 2.8. Evaluation of clinical samples. We obtained patient samples containing NPs of influenza A and B viruses from Discovery Life Sciences, Inc (Huntsville, AL, USA) | Full Paper | |
2020 | Seyger, M;Abramovits, W;Liljedahl, M;Hoejen, MN;Teng, J; | Safety and efficacy of fixed-dose combination calcipotriol (50 μg/g) and betamethasone dipropionate (0.5 mg/g) cutaneous foam in adolescent patients (aged 12 to <17 years) with plaque psoriasis: results of a phase II, open-label trial | J Eur Acad Dermatol Venereol | 32074665 | W. Abramovits has received honoraria or fees for serving on advisory boards as a speaker and as a consultant, and has received grants as an investigator from AbbVie, Akros, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant Bio | Full Paper |
2020 | Piha-Paul, SA;Oh, DY;Ueno, M;Malka, D;Chung, HC;Nagrial, A;Kelley, RK;Ros, W;Italiano, A;Nakagawa, K;Rugo, HS;de Braud, F;Varga, AI;Hansen, A;Wang, H;Krishnan, S;Norwood, KG;Doi, T; | Efficacy and safety of pembrolizumab for the treatment of advanced biliary cancer: Results from the KEYNOTE-158 and KEYNOTE-028 studies | Int. J. Cancer | 32359091 | In KEYNOTE‐028, a prototype immunohistochemistry assay (QualTek, Goleta, California) was used to prospectively detect PD‐L1 expression, with positivity defined as membranous PD‐L1 expression in ≥1% of tumor and associated inflammatory cells or positi | Full Paper |
2020 | Agnello, G;Alters, SE;Rowlinson, SW; | Preclinical safety and antitumor activity of the arginine-degrading therapeutic enzyme pegzilarginase, a PEGylated, cobalt-substituted recombinant human arginase 1 | Transl Res | 31954097 | CD8 IHC analysis of FFPE sections was performed by QualTek Molecular Laboratories (Goleta, Calif) and LC3B IHC analysis of FFPE sections was performed by GoPath Laboratories (Buffalo Grove, Ill). Statistical analyses | Full Paper |
2020 | Ross, AE;Hurley, PJ;Tran, PT;Rowe, SP;Benzon, B;Neal, TO;Chapman, C;Harb, R;Milman, Y;Trock, BJ;Drake, CG;Antonarakis, ES; | A pilot trial of pembrolizumab plus prostatic cryotherapy for men with newly diagnosed oligometastatic hormone-sensitive prostate cancer | Prostate Cancer Prostatic Dis. | 31611635 | pathologist. Post-treatment biopsies were evaluated for PD-L1, CD4, CD8, and FoxP3 proteins by single-stain semiquantitative immunohistochemistry performed by QualTek Molecular and Clinical Laboratories (Santa Barbara, CA) ;re assessed by a geni | Full Paper |
2020 | Scheffler, M;Holzem, A;Kron, A;Nogova, L;Ihle, MA;von Levetzow, C;Fassunke, J;Wömpner, C;Bitter, E;Koleczko, S;Abdulla, DSY;Michels, S;Fischer, R;Riedel, R;Weber, JP;Westphal, T;Gerigk, U;Kern, J;Kaminsky, B;Randerath, W;Kambartel, KO;Merkelbach-Bruse, S;Büttner, R;Wolf, J; | Co-occurrence of targetable mutations in Non-small cell lung cancer (NSCLC) patients harboring MAP2K1 mutations | Lung Cancer | 32361034 | Reinhard Büttner: RB has been a consultant for Pfizer and Novartis regarding MET inhibitors and is a co-founder and CSO of Targos Molecular Pathology, Kassel Germany | Full Paper |
2020 | Lee, EK;Xiong, N;Cheng, SC;Barry, WT;Penson, RT;Konstantinopoulos, PA;Hoffman, MA;Horowitz, N;Dizon, DS;Stover, EH;Wright, AA;Campos, SM;Krasner, C;Morrissey, S;Whalen, C;Quinn, R;Matulonis, UA;Liu, JF; | Combined pembrolizumab and pegylated liposomal doxorubicin in platinum resistant ovarian cancer: A phase 2 clinical trial | Gynecol. Oncol. | 32771276 | Tumor infiltrating lymphocytes (TILs) were estimated as an overall average of the sample and reported on a scale of 0 (absent TILs) to 3 (profuse infiltration). PD-L1 and TIL analysis was performed at QualTek Molecular Laboraties, PA | Full Paper |
2020 | Frumovitz, M;Westin, SN;Salvo, G;Zarifa, A;Xu, M;Yap, TA;Rodon, AJ;Karp, DD;Abonofal, A;Jazaeri, AA;Naing, A; | Phase II study of pembrolizumab efficacy and safety in women with recurrent small cell neuroendocrine carcinoma of the lower genital tract | Gynecol. Oncol. | 32534809 | Adverse events were assessed using the National Cancer Institute Common Terminology Criteria for Adverse Events version 4.03. When fresh or archived tissue was available, PD-L1 biomarker analysis was performed by Qualtek assay using Merck 22C3 antibo | Full Paper |
2020 | Gaillard, S;Broadwater, G;Andrews, W;Starr, M;Previs, R;Havrilesky, L;Davidson, B;Berchuck, A;Yi, J;Nixon, A;Secord, A; | Pembrolizumab window study: Illuminating the immunologic landscape in gynecologic cancers | Gynecologic Oncology | changes in PD-L1 expression (by immunohistochemistry with monoclonal antibody clone 22C3 on FFPE specimens;l Qualtek, PA) scored using a quantitative modified proportion score (MPS) | Full Paper | |
2020 | Pillarisetti, K;Powers, G;Luistro, L;Babich, A;Baldwin, E;Li, Y;Zhang, X;Mendonça, M;Majewski, N;Nanjunda, R;Chin, D;Packman, K;Elsayed, Y;Attar, R;Gaudet, F; | Teclistamab is an active T cell-redirecting bispecific antibody against B-cell maturation antigen for multiple myeloma | Blood Adv | 32956453 | Plasma samples were from normal subjects (n = 39) and patients with MM (n = 40); donors were acquired from Conversant Biologics and subjected to proteolysis and subsequent mass spectrometry using a reference multiple reaction monitoring sequence (YCN | Full Paper |
2020 | Svrzikapa, N;Longo, K;Prasad, N;Boyanapalli, R;Brown, J;Dorset, D;Yourstone, S;Powers, J;Levy, S;Morris, A;Vargeese, C;Goyal, J; | Investigational assay for haplotype phasing of the huntingtin gene | Molecular Therapy - Methods & Clinical Development | 4 1 Wave Life Sciences Ltd., Cambridge, MA, 02138 USA; 2 Department of Paediatrics, Medical 5 Sciences Division, University of Oxford, Oxford, OX3 9DU UK; 3 HudsonAlpha Discovery, 6 Discovery Life Sciences, Huntsville, AL, 35806 USA; 4 Genomic Servic | Full Paper | |
2020 | Sebest, P;Fojt, L;Ostatna, V;Fojta, M;Danhel, A; | Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent protein | Bioelectrochemistry | 31855832 | electrode setup composed of: i) a bPGE or edge-plane PGE (ePGE, both Momentive) working electrode, together with ii) a refillable miniature Ag/AgCl/3M KCl reference electrode (eDAQ) placed into the salt bridge composed of a heat shrink Teflon tube (Q | Full Paper |
2020 | Xicoy, H;Brouwers, JF;Wieringa, B;Martens, GJM; | Explorative Combined Lipid and Transcriptomic Profiling of Substantia Nigra and Putamen in Parkinson's Disease | Cells | 32858884 | Total RNA samples were spectrophotometrically analyzed and their 260/280 ratio was typically above 1.9. RNA-seq directionality library preparation and sequencing with Poly (A) selection was performed by HudsonAlpha Genomic Services Laboratory (Huntsv | Full Paper |
2020 | Elgharably, H;Okamoto, T;Ayyat, KS;Niikawa, H;Meade, S;Farver, C;Chan, ER;Aldred, MA;McCurry, KR; | Human Lungs Airway Epithelium Upregulate MicroRNA-17 and MicroRNA-548b in Response to Cold Ischemia and Ex Vivo Reperfusion | Transplantation | 32590607 | We thank Tracey Bonfield, PhD from CWRU for performing the cytokines analysis. We thank Gerard J. Nuovo, MD, and Adel Mikhail, PhD from Phylogeny Inc. for performing the in situ hybridization assays of the microRNA | Full Paper |
2020 | Grigorieva, J;Asmellash, S;Net, L;Tsypin, M;Roder, H;Roder, J; | Mass Spectrometry-Based Multivariate Proteomic Tests for Prediction of Outcomes on Immune Checkpoint Blockade Therapy: The Modern Analytical Approach | Int J Mol Sci | 32012941 | 50] PSEA reference set NSCLC patients; samples obtained from commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrix (Fort Worth, TX) 100 Grigorieva et al., 2019 [51] 3. BDX008 Test and ICB Test BDX008 | Full Paper |
2020 | Awty-Carroll, D;Ravella, S;Clifton-Brown, J;Robson, P; | Using a Taguchi DOE to investigate factors and interactions affecting germination in Miscanthus sinensis | Sci Rep | 32005862 | All dishes were prepared and tested at the same time to limit extraneous sources of variation. The Taguchi design of experiment (DOE) was produced and primarily analysed using Qualtek-4 (Nutek inc., Michigan, USA) with secondary analysis in R34 ;Ful | Full Paper |
2020 | Noto, PB;Sikorski, TW;Zappacosta, F;Wagner, CD;Montes de Oca, R;Szapacs, ME;Annan, RS;Liu, Y;McHugh, CF;Mohammad, HP;Piccoli, SP;Creasy, CL; | Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissues | Scientific reports | 33335114 | nd Treatment of Laboratory Animals and were reviewed by the Institutional Animal Care and Use Committee at GSK. Immunohistochemistry Both total and ADM-R225-hnRNP-A1 IHC assays were developed and at QualTek Molecular Laboratories using anti-hnRNP-A1 | Full Paper |
2020 | Rüschoff, J;Lebeau, A;Sinn, P;Schildhaus, HU;Decker, T;Ammann, J;Künzel, C;Koch, W;Untch, M; | Statistical modelling of HER2-positivity in breast cancer: Final analyses from two large, multicentre, non-interventional studies in Germany | Breast | 31918324 | Institut für Pathologie Nordhessen and Targos Molecular Pathology GmbH, Germaniastr. 7, D-34119, Kassel, Germany. Electronic address: rueschoff@patho-nordhessen.de.++Institut für Pathologie, Universitätsklinikum Hamburg-Eppendorf, Martinistr. 52, 202 | Full Paper |
2020 | Emancipator, K; | Keytruda and PD-L1: a Real-World Example of Co-development of a Drug with a Predictive Biomarker | The AAPS journal | 33222057 | two clinical trial assays were used (Table I). The first, described in the literature as the "prototype assay" (4), was developed in-house at Merck & Co., Inc., Kenilworth, NJ, USA, and eventually placed and refined in an accredited clinical laborato | Full Paper |
2020 | Gebauer, F;Krämer, M;Bruns, C;Schlößer, HA;Thelen, M;Lohneis, P;Schröder, W;Zander, T;Alakus, H;Buettner, R;Loeser, H;Quaas, A; | Lymphocyte activation gene-3 (LAG3) mRNA and protein expression on tumour infiltrating lymphocytes (TILs) in oesophageal adenocarcinoma | J. Cancer Res. Clin. Oncol. | 32592066 | Reinhard Büttner: Co-Founder and Co-Owner of Targos Molecular Pathology Inc. Kassel/Germany. Hans Schlösser: Funding for Research from Astra Zeneca for research outside this study. Thomas Zander: BMS: advisory board, clinical trial; Novartis: advisor | Full Paper |
2020 | Wang, X;Jaimes, M;Gu, H;Shults, K;Putta, S;Sharma, V;Chow, W;Gogoi, P;Handique, K;Patterson, BK; | Cell by cell immuno- and cancer marker profiling of non-small cell lung cancer tissue: Checkpoint marker expression on CD103+, CD4+ T-cells predicts circulating tumor cells | Translational oncology | 33217647 | of a total of 72 biomarkers that result from using combinations of cellular markers in our algorithm to predict CTCs. Material and methods Samples Fresh tissues were obtained from 10 NSCLC cases by Folio Biosciences [Powell, OH] with paired peripher | Full Paper |
2020 | MacCarty, N;Bentson, S;Cushman, K;Au, J;Li, C;Murugan, G;Still, D; | Stratification of particulate matter in a kitchen: A comparison of empirical to predicted concentrations and implications for cookstove emissions targets | Energy for Sustainable Development | the top perimeter (Fig. 3). Small computer fans (Qualtek FAD1-06020CSHW11 rated at 1.44 W @12 V and 16 CFM) covered the holes at the top perimeter to force air out of the test kitchen at a constant rate. In Phase 1 of the | Full Paper | |
2020 | Jia, Y;Liu, L;Shan, B; | Future of immune checkpoint inhibitors: focus on tumor immune microenvironment | Annals of translational medicine | 33145314 | The TNBC cohort in KEYNOTE-012 phase Ib trial enrolled 32 cases of heavily pre-treated patients with PD-L1 positivity in the stroma or PD-L1 ≥1% tumor cells using their Qualtek assay | Full Paper |
2020 | Sebest, P;Ostatna, V;Fojta, M;Danhel, A; | Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particles | Journal of Electroanalytical Chemistry | in three-electrode setup composed of: i) base plane PGE (bPGE,od 3.0 mm, Momentive) as working electrode, together with ii) refillable miniature Ag/AgCl/3 M KCl reference electrode (eDAQ) placed into the salt bridge composed of heat shrink Teflon tub | Full Paper | |
2020 | Rehkaemper, J;Korenkov, M;Quaas, A;Rueschoff, J;Pamuk, A;Zander, T;Hillmer, AM;Buettner, R;Hoelscher, AH;Bruns, CJ;Loeser, H;Alakus, H;Schoemig-Markiefka, B; | Amplification of KRAS and its heterogeneity in non-Asian gastric adenocarcinomas | BMC Cancer | 32571252 | Institute of Pathology, University Hospital Cologne, Cologne, Germany. jan.rehkaemper@uk-koeln.de.++Department of General, Visceral and Cancer Surgery, University Hospital Cologne, Cologne, Germany.++Institute of Pathology, University Hospital Cologn | Full Paper |
2020 | Montalban-Bravo, G;Class, CA;Ganan-Gomez, I;Kanagal-Shamanna, R;Sasaki, K;Richard-Carpentier, G;Naqvi, K;Wei, Y;Yang, H;Soltysiak, KA;Chien, K;Bueso-Ramos, C;Do, KA;Kantarjian, H;Garcia-Manero, G; | Transcriptomic analysis implicates necroptosis in disease progression and prognosis in myelodysplastic syndromes | Leukemia | 31719677 | BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). Patient characteristics are detailed in Supplementary Table S1. Informed consent was obtained Save | Full Paper |
2020 | Arumugam, A;Faron, ML;Yu, P;Markham, C;Wu, M;Wong, S; | A Rapid SARS-CoV-2 RT-PCR Assay for Low Resource Settings | Diagnostics (Basel, Switzerland) | 32987722 | No. 10006606) were purchased from Integrated DNA Technologies (Coralville, IA, USA). The clinical specimens of Influenza A (DLS16-85584), Influenza B (DLS15-33890), and RSV (KH19-00715) were obtained from Discovery Life Sciences Inc Cached | Full Paper |
2020 | Bio, S;Nunes, B; | Acute effects of diclofenac on zebrafish: Indications of oxidative effects and damages at environmentally realistic levels of exposure | Environ. Toxicol. Pharmacol. | 32320907 | With the increasing awareness about the contamination of the aquatic environment by pharmaceuticals, there is a growing need to study their adverse effects on aquatic organisms. Diclofenac is a non-steroidal anti-inflammatory drug (NSAID), whose wide | Full Paper |
2020 | Piedade, F;Bio, S;Nunes, B; | Effects of common pharmaceutical drugs (paracetamol and acetylsalicylic acid) short term exposure on biomarkers of the mussel Mytilus spp | Environ. Toxicol. Pharmacol. | 31704586 | Pharmaceutical drugs in the wild may pose significant risks to non-target exposed organisms. This situation is even more troublesome for coastal marine or estuarine environments, located in the vicinity of large human conglomerates, for which the put | Full Paper |
2020 | Lin, J;Song, AJ;Hoffman-Censits, J;Leiby, BE;Tuluc, M;Shaw, C;Harshyne, L;Kean, R;Bar-Ad, V;Den, RB;Hurwitz, MD;Louie, J;Philipose, S;Deshmukh, SP;Johnson, JM;Dicker, AP;Hooper, DC;Kelly, WK;Lu, B; | A Pilot Study of Radiation Therapy in Combination With Pembrolizumab in Patients With Metastatic Renal Cell Cancer | Am. J. Clin. Oncol. | 31693508 | When possible preradiation and post- radiation biopsy material was compared. Biopsy samples were analyzed by a QualTek pathologist (King of Prussia, PA) and Merck 22C3 antibody was used to score PD-L1 membrane expression | Full Paper |
2020 | Johnston, G;Ramsey, HE;Liu, Q;Wang, J;Stengel, KR;Sampathi, S;Acharya, P;Arrate, M;Stubbs, MC;Burn, T;Savona, MR;Hiebert, SW; | Nascent transcript and single-cell RNA-seq analysis defines the mechanism of action of the LSD1 inhibitor INCB059872 in myeloid leukemia | Gene | 32422235 | Samples were submitted to HudsonAlpha Genomic Services Laboratory for library preparation with Nugen Ovation RNA-Seq System V2 kit (cat# 7102-08) and sequencing on Illumina NovaSeq6000. Data analysis was performed as described above. 2.13 Data availa | Full Paper |
2020 | Lin, H;Damen, JE;Walasek, MA;Szilvassy, SJ;Turhan, AG;Louis, SA;Eaves, AC;Wognum, AW; | Feeder-free and serum-free in vitro assay for measuring the effect of drugs on acute and chronic myeloid leukemia stem/progenitor cells | Exp. Hematol. | 32798646 | CML. 1, Folio Conversant, 3.64 × 10 6, 4.9%, 81.2%, 15 × 10 3, 84, Male, Pre treatment, 112.2; 1764.4. 2, Folio Conversant, 3.17 × 10 6, 6.8%, 79.3%, 18.5 × 10 3, 70, Male, Pre treatment, 44.3; 2380.1. 3, Folio Conversant, 1.38 × 10 6, 18.8%, 78.7%, | Full Paper |
2020 | Barroso-Sousa, R;Krop, IE;Trippa, L;Tan-Wasielewski, Z;Li, T;Osmani, W;Andrews, C;Dillon, D;Richardson, ET;Pastorello, RG;Winer, EP;Mittendorf, EA;Bellon, JR;Schoenfeld, JD;Tolaney, SM; | A Phase II Study of Pembrolizumab in Combination With Palliative Radiotherapy for Hormone Receptor-positive Metastatic Breast Cancer | Clin. Breast Cancer | 32113750 | Clinical benefit is defined as complete response, partial response, or stable disease ≥ 24 weeks according to RECIST 1.1. Exploratory Biomarkers. Five patients had PD-L1 testing assessed centrally by QualTek using the 22C3 antibody | Full Paper |
2020 | Patel, JJ;Levy, DA;Nguyen, SA;Knochelmann, HM;Day, TA; | Impact of PD-L1 expression and human papillomavirus status in anti-PD1/PDL1 immunotherapy for head and neck squamous cell carcinoma-Systematic review and meta-analysis | Head Neck | 31762164 | Oro Valley, Arizona). One study28 did not specify the assay utilized for PD‐L1 expression, but rather reported the laboratory where analysis occurred (QualTek Molecular Laboratories, Goleta, California). This lab confirmed the | Full Paper |
2020 | Adekanmbi, EO;Giduthuri, AT;Srivastava, SK; | Dielectric Characterization and Separation Optimization of Infiltrating Ductal Adenocarcinoma via Insulator-Dielectrophoresis | Micromachines (Basel) | 32218322 | The DEP suspending medium (dextrose solution) was prepared and characterized as described in our previous article [38]. The prepared 100 mL suspending medium was divided into five separate beakers. Into each beaker, except the first, calculated volum | Full Paper |
2020 | Jasani, B;Bänfer, G;Fish, R;Waelput, W;Sucaet, Y;Barker, C;Whiteley, JL;Walker, J;Hovelinck, R;Diezko, R; | Evaluation of an online training tool for scoring programmed cell death ligand-1 (PD-L1) diagnostic tests for lung cancer | Diagn Pathol | 32303234 | Correspondence: rolf.diezko@targos-gmbh.de 2Advance - Training and Consulting Unit, Targos Molecular Pathology GmbH, Kassel, Germany Full list of author information is available at the end of the article Jasani et al ;Correspondence: rolf.diezko | Full Paper |
2020 | Schöniger, S;Degner, S;Zhang, Q;Schandelmaier, C;Aupperle-Lellbach, H;Jasani, B;Schoon, HA; | Tumor Infiltrating Lymphocytes in Pet Rabbit Mammary Carcinomas: A Study with Relevance to Comparative Pathology | Animals : an open access journal from MDPI | 32824521 | 1. Targos Molecular Pathology GmbH, Germaniastrasse 7, 34119 Kassel, Germany. 2. Institute of Veterinary Pathology, University of Leipzig, An den Tierkliniken, 04109 Leipzig, Germany. 3. Institute of Anatomy, Experimental Neurobiology Cached | Full Paper |
2020 | Schöniger, S;Schoon, HA; | The Healthy and Diseased Equine Endometrium: A Review of Morphological Features and Molecular Analyses | Animals : an open access journal from MDPI | 32260515 | Targos Molecular Pathology GmbH, Germaniastrasse 7, 34119 Kassel, Germany.++Institute of Veterinary Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig, Germany | Full Paper |
2020 | Fish, EJ;Martinez-Romero, EG;DeInnocentes, P;Koehler, JW;Prasad, N;Smith, AN;Bird, RC; | Circulating microRNA as biomarkers of canine mammary carcinoma in dogs | J. Vet. Intern. Med. | 32342546 | Extracted RNA was stored at −80°C until being shipped on dry ice to the Genomic Services Laboratory at HudsonAlpha Discovery for deep‐sequencing as previously described.14 Briefly, RNA libraries were processed extracted RNA using a NEBNext Small RNA | Full Paper |
2020 | Nuovo, G; | A broad-based approach to differentiate CIN from its mimics: The utility of in situ hybridization and immunohistochemistry | Ann Diagn Pathol | 32330660 | study was the review of 100 consecutive cases of formalin fixed, paraffin embedded tissues cervical biopsies done for either an abnormal Pap smear and/or a high risk HPV DNA positive result where the diagnosis was listed as CIN 1 or CIN 2. Folio Bios | Full Paper |
2020 | Inceer, H;Ozcan, M; | Taxonomic evaluations on the anatomical characters of leaf and achene in Turkish Tripleurospermum with its relative Matricaria (Asteraceae) | Flora | Besides, both genera have appeared well separated in different and supported clades based on molecular phylogeny (Inceer et al., 2018) | Full Paper | |
2020 | Roder, J;Net, L;Oliveira, C;Meyer, K;Asmellash, S;Kasimir-Bauer, S;Pass, H;Weber, J;Roder, H;Grigorieva, J; | A proposal for score assignment to characterize biological processes from mass spectral analysis of serum | Clinical Mass Spectrometry | Samples were obtained from the commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrics (Fort Worth, TX). b They were purchased from commercial biobanks, including Conversant Bio (Huntsville, AL), AdeptBio (Memphis, TN), and ProMedD | Full Paper | |
2020 | Köhne, M;Mönnig, F;Papin, J;Schöniger, S;Tönissen, A;Rohn, K;Martinsson, G;Schoon, HA;Sieme, H; | Effects of Hysteroscopic and Uterine Body Insemination on the Presence of Selected Immune Cells in the Equine Endometrium | J. Equine Vet. Sci. | 32534786 | Current affiliation: Targos Molecular Pathology GmbH, 34119 Kassel, Germany | Full Paper |
2020 | Schildhaus, HU; | [Immunohistochemistry-based predictive biomarkers for lung cancer] | Pathologe | 31989233 | Interessenkonflikt. H.-U. Schildhaus erhielt Honorare von MSD, BMS, Abbvie, ZytoVision, Zytomed, Pfizer, Abbott Molecular, Novartis Oncology und Roche. Der Autor ist Board Member bzw. Assessor bei QuIP, NordiQC und UKNEQAS sowie Angestellter der Targ | Full Paper |
2020 | Tsai, AG;Glass, DR;Juntilla, M;Hartmann, FJ;Oak, JS;Fernandez-Pol, S;Ohgami, RS;Bendall, SC; | Multiplexed single-cell morphometry for hematopathology diagnostics | Nature medicine | 32161403 | Healthy bone marrow samples from AllCells were ordered, delivered and processed the same day. Healthy bone marrow 1 and 2 refer to the AllCells 9878 and 10874 samples, Save | Full Paper |
2020 | Reyes, M;Filbin, MR;Bhattacharyya, RP;Billman, K;Eisenhaure, T;Hung, DT;Levy, BD;Baron, RM;Blainey, PC;Goldberg, MB;Hacohen, N; | An immune-cell signature of bacterial sepsis | Nature medicine | 32066974 | De-identified BMMC samples were purchased from AllCells or Stemcell Technologies. This study was approved by the Institutional Review Boards at the Broad Institute of MIT and Save | Full Paper |
2020 | Travaglini, KJ;Nabhan, AN;Penland, L;Sinha, R;Gillich, A;Sit, RV;Chang, S;Conley, SD;Mori, Y;Seita, J;Berry, GJ;Shrager, JB;Metzger, RJ;Kuo, CS;Neff, N;Weissman, IL;Quake, SR;Krasnow, MA; | A molecular cell atlas of the human lung from single-cell RNA sequencing | Nature | 33208946 | For bulk RNA-seq of canonical immune populations, whole blood from healthy human donors was obtained commericially (AllCells) in EDTA tubes. Patient tissues were obtained under Save | Full Paper |
2020 | Link, JO;Rhee, MS;Tse, WC;Zheng, J;Somoza, JR;Rowe, W;Begley, R;Chiu, A;Mulato, A;Hansen, D;Singer, E;Tsai, LK;Bam, RA;Chou, CH;Canales, E;Brizgys, G;Zhang, JR;Li, J;Graupe, M;Morganelli, P;Liu, Q;Wu, Q;Halcomb, RL;Saito, RD;Schroeder, SD;Lazerwith, SE;Bondy, S;Jin, D;Hung, M;Novikov, N;Liu, X;Villaseñor, AG;Cannizzaro, CE;Hu, EY;Anderson, RL;Appleby, TC;Lu, B;Mwangi, J;Liclican, A;Niedziela-Majka, A;Papalia, GA;Wong, MH;Leavitt, SA;Xu, Y;Koditek, D;Stepan, GJ;Yu, H;Pagratis, N;Clancy, S;Ahmadyar, S;Cai, TZ;Sellers, S;Wolckenhauer, SA;Ling, J;Callebaut, C;Margot, N;Ram, RR;Liu, YP;Hyland, R;Sinclair, GI;Ruane, PJ;Crofoot, GE;McDonald, CK;Brainard, DM;Lad, L;Swaminathan, S;Sundquist, WI;Sakowicz, R;Chester, AE;Lee, WE;Daar, ES;Yant, SR;Cihlar, T; | Clinical targeting of HIV capsid protein with a long-acting small molecule | Nature | 32612233 | Human peripheral blood mononuclear cells (PBMCs) were collected from healthy volunteers under informed consent; their use was approved by an institutional review board at AllCells Save | Full Paper |
2020 | Ferrari, S;Jacob, A;Beretta, S;Unali, G;Albano, L;Vavassori, V;Cittaro, D;Lazarevic, D;Brombin, C;Cugnata, F;Kajaste-Rudnitski, A;Merelli, I;Genovese, P;Naldini, L; | Efficient gene editing of human long-term hematopoietic stem cells validated by clonal tracking | Nature biotechnology | 32601433 | (Biovision) and 50 nM UM171 (STEMCell Technologies), unless otherwise specified. G-CSF mPB CD34+ HSPCs were purified with the CliniMACS CD34 Reagent System (Miltenyi Biotec) from Mobilized Leukopak (AllCells) upon approval by the Ospedale San Raffae | Full Paper |
2020 | Spindler, MJ;Nelson, AL;Wagner, EK;Oppermans, N;Bridgeman, JS;Heather, JM;Adler, AS;Asensio, MA;Edgar, RC;Lim, YW;Meyer, EH;Hawkins, RE;Cobbold, M;Johnson, DS; | Massively parallel interrogation and mining of natively paired human TCRαβ repertoires | Nature biotechnology | 32393905 | novel ACT. Online Methods Sourcing and Processing Human Materials De-identified peripheral blood mononuclear cells (PBMCs) in leukopaks were obtained from HLA-A*02:01 and HLA-A*24:02 healthy donors (AllCells, Alameda, CA, USA), under an Institutiona | Full Paper |
2020 | Dinh, HQ;Eggert, T;Meyer, MA;Zhu, YP;Olingy, CE;Llewellyn, R;Wu, R;Hedrick, CC; | Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone Marrow | Immunity | 32814027 | Human bone marrow cells Fresh bone marrow samples from anonymous healthy adult donors were obtained from AllCells, Inc. (Alameda, CA). For information on donor characteristics, refer to Supplemental Table 1. Upon arrival, the fresh cells were immedia | Full Paper |
2020 | Glass, DR;Tsai, AG;Oliveria, JP;Hartmann, FJ;Kimmey, SC;Calderon, AA;Borges, L;Glass, MC;Wagar, LE;Davis, MM;Bendall, SC; | An Integrated Multi-omic Single-Cell Atlas of Human B Cell Identity | Immunity | 32668225 | structive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com ------------------------- | Full Paper |
2020 | Park, HB;Wei, Z;Oh, J;Xu, H;Kim, CS;Wang, R;Wyche, TP;Piizzi, G;Flavell, RA;Crawford, JM; | Sulfamethoxazole drug stress upregulates antioxidant immunomodulatory metabolites in Escherichia coli | Nature microbiology | 32719505 | ndors supply Eurofins DiscoverX with primary human cells: Cell Applications, Inc., CellzDirect, Celsis-IVT, Leukolab, Life Technologies, Lonza, ScienCell, Hemacare Corporation, Stemcell Technologies, AllCells, Physician’s Plasma Alliance, Lifeline Ce | Full Paper |
2020 | Di Genua, C;Valletta, S;Buono, M;Stoilova, B;Sweeney, C;Rodriguez-Meira, A;Grover, A;Drissen, R;Meng, Y;Beveridge, R;Aboukhalil, Z;Karamitros, D;Belderbos, ME;Bystrykh, L;Thongjuea, S;Vyas, P;Nerlov, C; | C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid Progenitor | Cancer cell | 32330454 | derbos et al., 2017) ------------------------- Biological Samples ------------------------- AEL patient samples (OX1164; AYL050; MKH048; STB115) MDSBio NA Normal adult human bone marrow AllCells NA ------------------------- Critical | Full Paper |
2020 | Li, S;Chen, X;Wang, J;Meydan, C;Glass, JL;Shih, AH;Delwel, R;Levine, RL;Mason, CE;Melnick, AM; | Somatic Mutations Drive Specific, but Reversible, Epigenetic Heterogeneity States in AML | Cancer discovery | 32938585 | from our prior study(9), 5 of which were purchased from AllCells (Alameda, CA, USA) and 9 of which were isolated using magnetic bead positive selection for CD34+ (Miltenyi Biotec) from freshly collected bone marrow samples from individuals without kn | Full Paper |
2020 | Zhou, Y;Han, C;Wang, E;Lorch, AH;Serafin, V;Cho, BK;Gutierrez Diaz, BT;Calvo, J;Fang, C;Khodadadi-Jamayran, A;Tabaglio, T;Marier, C;Kuchmiy, A;Sun, L;Yacu, G;Filip, SK;Jin, Q;Takahashi, YH;Amici, DR;Rendleman, EJ;Rawat, R;Bresolin, S;Paganin, M;Zhang, C;Li, H;Kandela, I;Politanska, Y;Abdala-Valencia, H;Mendillo, ML;Zhu, P;Palhais, B;Van Vlierberghe, P;Taghon, T;Aifantis, I;Goo, YA;Guccione, E;Heguy, A;Tsirigos, A;Wee, KB;Mishra, RK;Pflumio, F;Accordi, B;Basso, G;Ntziachristos, P; | Posttranslational Regulation of the Exon Skipping Machinery Controls Aberrant Splicing in Leukemia | Cancer discovery | 32444465 | Our study provides a new proof-of-principle for posttranslational regulation of splicing factors independently of mutations in aggressive T-cell leukemia. It further suggests a new drug Save | Full Paper |
2020 | Tochigi, T;Miyamoto, T;Hatakeyama, K;Sakoda, T;Ishihara, D;Irifune, H;Shima, T;Kato, K;Maeda, T;Ito, T;Handa, H;Akashi, K;Kikushige, Y; | Aromatase is a novel neosubstrate of cereblon responsible for immunomodulatory drug-induced thrombocytopenia | Blood | 32219443 | Human adult bone marrow and granulocyte colony-stimulating factor-mobilized peripheral blood samples were obtained from healthy donors or purchased from AllCells. Cord blood Save | Full Paper |
2020 | Montalban Bravo, G;Kanagal-Shamanna, R;Darbaniyan, F;Ganan-Gomez, I;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, K;Chien, K;Bueso-Ramos, C;Kantarjian, H;Garcia-Manero, G; | Genomic and Transcriptomic Differences of Myelodysplastic Syndrome/Myeloproliferative Neoplasm with Ring Sideroblasts and Thrombocytosis (MDS/MPN-RS-T) and Myelodysplastic Syndrome with Ring Sideroblasts (MDS-RS) | Blood | CD34+ cells from bone marrow samples of 4 pts with MDS/MPN-RS-T, 7 pts with MDS-RS and 17 healthy individuals obtained from AllCells (Emeryville, CA) were isolated using the Save | Full Paper | |
2020 | Montalban Bravo, G;Darbaniyan, F;Kanagal-Shamanna, R;Ganan-Gomez, I;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, K;Chien, K;Bueso-Ramos, C;Kantarjian, H;Garcia-Manero, G; | Transcriptomic Features and Deregulation of Genes Involved in Monopoiesis Influence Outcomes of Patients with Chronic Myelomonocytic Leukemia | Blood | INTRODUCTION: Chronic myelomonocytic leukemia (CMML) is characterized by TET2, SRSF2, ASXL1 and RAS pathway mutations known to induce myelomonocytic bias. We have previously shown that upregulation of KDM6B, a histone demethylase that acts as an inna | Full Paper | |
2020 | Walters, M;Chui, D;Farrell, J;Lal, A;Locatelli, F;Kwiatkowski, J;Porter, J;Sauer, M;Thuret, I;Hongeng, S;Kulozik, A;Thrasher, A;Yannaki, E;Yang, J;Whitney, D;Petrusich, A;Colvin, R;Thompson, A; | Response of Patients with Transfusion-Dependent β-Thalassemia (TDT) to Betibeglogene Autotemcel (beti-cel; LentiGlobin for β-Thalassemia) Gene Therapy Based on HBB Genotype and Disease Genetic Modifiers | Blood | Introduction We investigated the impact of β-thalassemia genotypes and disease genetic modifiers including HBA and KLF1 genotype and sentinel single-nucleotide polymorphism (SNP) genotypes at 3 major HbF quantitative trait loci (QTL) o | Full Paper | |
2020 | Kanter, J;Tisdale, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Chen, R;Gallagher, M;Ding, Y;Goyal, S;Paramore, C;Thompson, A;Walters, M; | Improvements in Health-Related Quality of Life for Patients Treated with LentiGlobin for Sickle Cell Disease (bb1111) Gene Therapy | Blood | Background In patients with sickle cell disease (SCD), health-related quality of life (HRQoL) is worse than in the general population and comparable or worse than in patients with other chronic or painful diseases such as cystic fibros | Full Paper | |
2020 | Thompson, A;Kwiatkowski, J;Porter, J;Hongeng, S;Yannaki, E;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Guo, R;Liu, W;Colvin, R;Walters, M;Locatelli, F; | Favorable Outcomes in Pediatric Patients in the Phase 3 Hgb-207 (Northstar-2) and Hgb-212 (Northstar-3) Studies of Betibeglogene Autotemcel Gene Therapy for the Treatment of Transfusion-Dependent β-Thalassemia | Blood | Introduction Betibeglogene autotemcel (beti-cel; LentiGlobin for β-thalassemia) gene therapy is being evaluated for the treatment of transfusion-dependent β-thalassemia (TDT). Initial positive results of beti-cel in the phase 3 studies | Full Paper | |
2020 | Kwiatkowski, J;Walters, M;Hongeng, S;Locatelli, F;Rasko, J;Cavazzana, M;Chen, Y;Colvin, R;Thompson, A; | Long-Term Efficacy and Safety of Betibeglogene Autotemcel Gene Therapy for the Treatment of Transfusion-Dependent β-Thalassemia: Results in Patients with up to 6 Years of Follow-up | Blood | Introduction The goal of betibeglogene autotemcel (beti-cel; LentiGlobin for β-thalassemia) gene therapy in patients with transfusion-dependent β-thalassemia (TDT) is lifelong, stable production of functional adult hemoglobin (Hb) suff | Full Paper | |
2020 | Thompson, A;Walters, M;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Schmidt, M;DelCarpini, J;Pierciey, F;Miller, A;Gallagher, M;Chen, R;Goyal, S;Kanter, J;Tisdale, J; | Resolution of Serious Vaso-Occlusive Pain Crises and Reduction in Patient-Reported Pain Intensity: Results from the Ongoing Phase 1/2 HGB-206 Group C Study of LentiGlobin for Sickle Cell Disease (bb1111) Gene Therapy | Blood | Background Sickle cell disease (SCD) is caused by abnormal sickle hemoglobin (HbS) and results in chronic hemolytic anemia, painful vaso-occlusive events (VOEs), and progressive vasculopathy that lead to significant morbidity. While ac | Full Paper | |
2020 | Rothe, K;Babaian, A;Nakamichi, N;Chen, M;Chafe, SC;Watanabe, A;Forrest, DL;Mager, DL;Eaves, CJ;Dedhar, S;Jiang, X; | Integrin-Linked Kinase Mediates Therapeutic Resistance of Quiescent CML Stem Cells to Tyrosine Kinase Inhibitors | Cell stem cell | 32413332 | Full Paper | |
2020 | Nakao, S;Arai, Y;Tasaki, M;Yamashita, M;Murakami, R;Kawase, T;Amino, N;Nakatake, M;Kurosaki, H;Mori, M;Takeuchi, M;Nakamura, T; | Intratumoral expression of IL-7 and IL-12 using an oncolytic virus increases systemic sensitivity to immune checkpoint blockade | Science translational medicine | 31941828 | hIL-7/hIL-12-VV at MOI 1.0 and cultured for 2 days. Then, the supernatant was filtered through 0.1-μm polyvinylidene difluoride membrane (Millipore) and added to 1 × 105 human PBMCs (AllCells) in a 96-well plate. Seven days later, secretion of human | Full Paper |
2020 | Geng, J;Liu, W;Zhou, H;Zhang, T;Wang, L;Zhang, M;Li, Y;Shen, B;Li, X;Xiao, B; | A Plug-and-Latch Mechanism for Gating the Mechanosensitive Piezo Channel | Neuron | 32142647 | HEK293T Xiao et al., 2011 N/A HEK293T-Piezo1-KO Cahalan et al., 2015 N/A HUVEC Allcells Cat# H-001F-C HeLa Xiao et al., 2011 N/A N2A Coste et al., 2010 N/A C2C12 National Infrastructure of Cell line Source, China Cat# GNM26 | Full Paper |
2020 | Fang, C;Wang, Z;Han, C;Safgren, SL;Helmin, KA;Adelman, ER;Serafin, V;Basso, G;Eagen, KP;Gaspar-Maia, A;Figueroa, ME;Singer, BD;Ratan, A;Ntziachristos, P;Zang, C; | Cancer-specific CTCF binding facilitates oncogenic transcriptional dysregulation | Genome biology | 32933554 | Human CD4 + T cells were purchased from AllCells. Primary human samples were collected with informed consent and analyzed under the supervision of the Institutional Review Board Save | Full Paper |
2020 | Li, M;Liu, W;Bauch, T;Graviss, EA;Arduino, RC;Kimata, JT;Chen, M;Wang, J; | Clearance of HIV infection by selective elimination of host cells capable of producing HIV | Nature communications | 32792548 | pecific-pathogen-free barrier animal facility at the Houston Methodist Research Institute. Newborn male and female mice were injected intrahepatically with CD34+ human stem cells (5 × 104/mouse; AllCells LLC). Three months later, reconstitution of hu | Full Paper |
2020 | Scharenberg, SG;Poletto, E;Lucot, KL;Colella, P;Sheikali, A;Montine, TJ;Porteus, MH;Gomez-Ospina, N; | Engineering monocyte/macrophage-specific glucocerebrosidase expression in human hematopoietic stem cells using genome editing | Nature communications | 32620863 | Human CD34+ HSPCs mobilized from peripheral blood were purchased frozen from AllCells (Almeda, CA, USA) and thawed per manufacturer's instructions. Human Cord blood was Save | Full Paper |
2020 | Wienert, B;Nguyen, DN;Guenther, A;Feng, SJ;Locke, MN;Wyman, SK;Shin, J;Kazane, KR;Gregory, GL;Carter, MAM;Wright, F;Conklin, BR;Marson, A;Richardson, CD;Corn, JE; | Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair | Nature communications | 32355159 | Cryopreserved wildtype human mobilized peripheral blood CD34 + HSPCs from volunteer donors were purchased from Allcells, and were cultured in SFEMII + CC110 (StemCell Save | Full Paper |
2020 | Lareau, CA;Ma, S;Duarte, FM;Buenrostro, JD; | Inference and effects of barcode multiplets in droplet-based single-cell assays | Nature communications | 32054859 | lues have been published, these values were approximated from an examination of a plot previously reported2. Profiling PBMCs using 10× scATAC-seq For 10× scATAC-seq experiments with PBMCs (PB003F, Allcells), frozen cells were quickly thawed in a 37 | Full Paper |
2020 | Mahuron, KM;Moreau, JM;Glasgow, JE;Boda, DP;Pauli, ML;Gouirand, V;Panjabi, L;Grewal, R;Luber, JM;Mathur, AN;Feldman, RM;Shifrut, E;Mehta, P;Lowe, MM;Alvarado, MD;Marson, A;Singer, M;Wells, J;Jupp, R;Daud, AI;Rosenblum, MD; | Layilin augments integrin activation to promote antitumor immunity | The Journal of experimental medicine | 32539073 | Human peripheral blood mononuclear cells (PBMCs) from two individual donors were purchased from AllCells. CD8 + T cells were enriched from these samples using a negative Save | Full Paper |
2020 | Poran, A;Scherer, J;Bushway, ME;Besada, R;Balogh, KN;Wanamaker, A;Williams, RG;Prabhakara, J;Ott, PA;Hu-Lieskovan, S;Khondker, ZS;Gaynor, RB;Rooney, MS;Srinivasan, L; | Combined TCR Repertoire Profiles and Blood Cell Phenotypes Predict Melanoma Patient Response to Personalized Neoantigen Therapy plus Anti-PD-1 | Cell reports. Medicine | 33294862 | Patient PBMCs from NT-001 trial Ott et al., 2020 N/A Healthy Donor PBMCs Precision for Medicine Cat # 93000-10M Healthy Donor PBMCs StemExpress Cat # LE010F Healthy Donor PBMCs AllCells https://www.allcells.com/tissue-products/ | Full Paper |
2020 | Huang, H;Zhu, H;Xie, Q;Tian, X;Yang, X;Feng, F;Jiang, Q;Sheng, X;Yang, Z; | Evaluation of 124I-JS001 for hPD1 immuno-PET imaging using sarcoma cell homografts in humanized mice | Acta pharmaceutica Sinica. B | 32874831 | The human OS-732 cell line was purchased from the Institute of Cancer, Chinese Academy of Medical Sciences (Beijing, China). Normal peripheral blood T cells and CD3+ pan T cells were obtained from AllCells (PB009-1F-C, Emeryville, CA, USA). Phytohema | Full Paper |
2020 | Poran, A;Harjanto, D;Malloy, M;Arieta, CM;Rothenberg, DA;Lenkala, D;van Buuren, MM;Addona, TA;Rooney, MS;Srinivasan, L;Gaynor, RB; | Sequence-based prediction of SARS-CoV-2 vaccine targets using a mass spectrometry-based bioinformatics predictor identifies immunogenic T cell epitopes | Genome medicine | 32791978 | Human PBMCs from HLA-A02:01-positive human donors were isolated using Ficoll separation from apheresis material (AllCells, USA). Twenty-three SARS-CoV-2 peptides predicted to Save | Full Paper |
2020 | Goodwin, M;Lee, E;Lakshmanan, U;Shipp, S;Froessl, L;Barzaghi, F;Passerini, L;Narula, M;Sheikali, A;Lee, CM;Bao, G;Bauer, CS;Miller, HK;Garcia-Lloret, M;Butte, MJ;Bertaina, A;Shah, A;Pavel-Dinu, M;Hendel, A;Porteus, M;Roncarolo, MG;Bacchetta, R; | CRISPR-based gene editing enables FOXP3 gene repair in IPEX patient cells | Science advances | 32494707 | families. Additional healthy donor HSPCs were isolated from umbilical cord blood donors provided by the Binns Program for Cord Blood Research at the Stanford University or purchased commercially from AllCells or StemCell Technologies. For HSPC isolat | Full Paper |
2020 | Grozdanovic, MM;Doyle, CB;Liu, L;Maybruck, BT;Kwatia, MA;Thiyagarajan, N;Acharya, KR;Ackerman, SJ; | Charcot-Leyden crystal protein/galectin-10 interacts with cationic ribonucleases and is required for eosinophil granulogenesis | The Journal of allergy and clinical immunology | 31982451 | Purified human CD34 + cord blood-derived hematopoietic progenitor cells were purchased from AllCells (Emeryville, Calif) and cultured in Iscove modified Dulbecco medium Save | Full Paper |
2020 | Lundin, V;Sugden, WW;Theodore, LN;Sousa, PM;Han, A;Chou, S;Wrighton, PJ;Cox, AG;Ingber, DE;Goessling, W;Daley, GQ;North, TE; | YAP Regulates Hematopoietic Stem Cell Formation in Response to the Biomechanical Forces of Blood Flow | Developmental cell | 32032546 | Cord Blood CD34+ Cells AllCells Cat. CB008F mPB CD34+ Cells AllCells Cat. mPB015F Mouse Embryonic Fibroblasts GlobalStem Cat. 6001G Primary Human Umbilical Vein Endothelial Cells ThermoFisher Cat. C0155C | Full Paper |
2020 | Duncan, RM;Reyes, L;Moats, K;Robinson, RM;Murphy, SA;Kaur, B;Stessman, HAF;Dolloff, NG; | ATF3 Coordinates Antitumor Synergy between Epigenetic Drugs and Protein Disulfide Isomerase Inhibitors | Cancer research | 32561529 | T cells were expanded from human peripheral blood monoleukocytes purchased from AllCells LLC (PB006F). All cells were maintained at 37C and 5% CO 2 . Experiments were Save | Full Paper |
2020 | Yeung, YA;Krishnamoorthy, V;Dettling, D;Sommer, C;Poulsen, K;Ni, I;Pham, A;Chen, W;Liao-Chan, S;Lindquist, K;Chin, SM;Chunyk, AG;Hu, W;Sasu, B;Chaparro-Riggers, J;Djuretic, I; | An Optimized Full-Length FLT3/CD3 Bispecific Antibody Demonstrates Potent Anti-leukemia Activity and Reversible Hematological Toxicity | Molecular therapy : the journal of the American Society of Gene Therapy | 31981494 | After tumor engraftment (day 6-7), animals were administered 2 × 10 7 expanded human T cells (T cells purchased from Allcells; activated according to Miltenyi manufacturing protocol) Save | Full Paper |
2020 | Morgan, RA;Unti, MJ;Aleshe, B;Brown, D;Osborne, KS;Koziol, C;Ayoub, PG;Smith, OB;O'Brien, R;Tam, C;Miyahira, E;Ruiz, M;Quintos, JP;Senadheera, S;Hollis, RP;Kohn, DB; | Improved Titer and Gene Transfer by Lentiviral Vectors Using Novel, Small β-Globin Locus Control Region Elements | Molecular therapy : the journal of the American Society of Gene Therapy | 31628051 | All BM aspirates were obtained from voluntary healthy donors supplied by AllCells (Alameda, CA), which obviated the need for Instituional Review Board review. BM mononuclear cells Save | Full Paper |
2020 | Sinnakannu, JR;Lee, KL;Cheng, S;Li, J;Yu, M;Tan, SP;Ong, CCH;Li, H;Than, H;Anczuków-Camarda, O;Krainer, AR;Roca, X;Rozen, SG;Iqbal, J;Yang, H;Chuah, C;Ong, ST; | SRSF1 mediates cytokine-induced impaired imatinib sensitivity in chronic myeloid leukemia | Leukemia | 32051529 | at the Singapore General Hospital, cord blood samples were obtained from the Singapore Cord Blood Bank, and normal bone marrow cells were obtained commercially from AllCells ( Save | Full Paper |
2020 | Korotchkina, L;Kazyulkin, D;Komarov, PG;Polinsky, A;Andrianova, EL;Joshi, S;Gupta, M;Vujcic, S;Kononov, E;Toshkov, I;Tian, Y;Krasnov, P;Chernov, MV;Veith, J;Antoch, MP;Middlemiss, S;Somers, K;Lock, RB;Norris, MD;Henderson, MJ;Haber, M;Chernova, OB;Gudkov, AV; | OT-82, a novel anticancer drug candidate that targets the strong dependence of hematological malignancies on NAD biosynthesis | Leukemia | 31896781 | Normal human bone marrow mononuclear cells (BM-MNC) were obtained from ALLCELLS, LLC (Almeda CA, USA). Patients’ BM-MNC were procured at the Hematologic Bank of RPCCC and NAMPT monoclonal antibody (clone OMNI 379) was obtained from Cayman Chemicals ( | Full Paper |
2020 | Kravtsova-Ivantsiv, Y;Goldhirsh, G;Ivantsiv, A;Ben Itzhak, O;Kwon, YT;Pikarsky, E;Ciechanover, A; | Excess of the NF-ĸB p50 subunit generated by the ubiquitin ligase KPC1 suppresses tumors via PD-L1- and chemokines-mediated mechanisms | Proceedings of the National Academy of Sciences of the United States of America | 33168738 | Human PBMCs as a source for isolating human NK cells were from AllCells. Mouse anti-CD274 (PD-L1) (29E.2A3) and mouse anti-CD45 (HI30) for mass cytometry were from Bio Save | Full Paper |
2020 | Ojaghi, A;Carrazana, G;Caruso, C;Abbas, A;Myers, DR;Lam, WA;Robles, FE; | Label-free hematology analysis using deep-ultraviolet microscopy | Proceedings of the National Academy of Sciences of the United States of America | 32561645 | bone marrow aspirate was collected from healthy donors (AllCells) and added to Na heparin anticoagulant solution. Smears were made on uncoated quartz slides using 10 μL of whole Save | Full Paper |
2020 | Huang, P;Zhao, Y;Zhong, J;Zhang, X;Liu, Q;Qiu, X;Chen, S;Yan, H;Hillyer, C;Mohandas, N;Pan, X;Xu, X; | Putative regulators for the continuum of erythroid differentiation revealed by single-cell transcriptome of human BM and UCB cells | Proceedings of the National Academy of Sciences of the United States of America | 32457162 | CD34 + stem/progenitor cells (AllCells) were obtained from three mobilized BM and three neonate UCB samples from independent donors. Cells were expanded at 37 C and 5% CO 2 Save | Full Paper |
2020 | Belling, JN;Heidenreich, LK;Tian, Z;Mendoza, AM;Chiou, TT;Gong, Y;Chen, NY;Young, TD;Wattanatorn, N;Park, JH;Scarabelli, L;Chiang, N;Takahashi, J;Young, SG;Stieg, AZ;De Oliveira, S;Huang, TJ;Weiss, PS;Jonas, SJ; | Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cells | Proceedings of the National Academy of Sciences of the United States of America | 32358194 | Mixed donor umbilical cord CD34+ Hematopoietic stem and progenitor cells (Allcells Inc.) were thawed and prestimulated as described by Hoban et al. (44). Postacoustofluidic treatment, HSPCs were cultured in X-VIVO 15 supplemented with 50 ng/μL recomb | Full Paper |
2020 | Borodovsky, A;Barbon, CM;Wang, Y;Ye, M;Prickett, L;Chandra, D;Shaw, J;Deng, N;Sachsenmeier, K;Clarke, JD;Linghu, B;Brown, GA;Brown, J;Congreve, M;Cheng, RK;Dore, AS;Hurrell, E;Shao, W;Woessner, R;Reimer, C;Drew, L;Fawell, S;Schuller, AG;Mele, DA; | Small molecule AZD4635 inhibitor of A2AR signaling rescues immune cell function including CD103+ dendritic cells enhancing anti-tumor immunity | Journal for immunotherapy of cancer | 32727810 | 01 individuals. As previously described,38 naïve CD8+ T cells were co-cultured with autologous monocyte-derived DCs, both cell populations isolated from PBMC from HLA-A*0201 positive healthy donors (AllCells, Research Blood Components). Monocytes wer | Full Paper |
2020 | Kotanides, H;Li, Y;Malabunga, M;Carpenito, C;Eastman, SW;Shen, Y;Wang, G;Inigo, I;Surguladze, D;Pennello, AL;Persaud, K;Hindi, S;Topper, M;Chen, X;Zhang, Y;Bulaon, DK;Bailey, T;Lao, Y;Han, B;Torgerson, S;Chin, D;Sonyi, A;Haidar, JN;Novosiadly, RD;Moxham, CM;Plowman, GD;Ludwig, DL;Kalos, M; | Bispecific Targeting of PD-1 and PD-L1 Enhances T-cell Activation and Antitumor Immunity | Cancer immunology research | 32873605 | Monocytes were isolated from frozen normal peripheral blood mononuclear cells (PBMC) purchased from AllCells (PB005F) using Monocyte Isolation Kit (Miltenyi Biotec; 130-091-153). Immature DCs were generated by culturing the monocytes in RPMI1640 medi | Full Paper |
2020 | Liu, Y;Lv, S;Liu, D;Song, F; | Recent development of amorphous metal coordination polymers for cancer therapy | Acta biomaterialia | 32942012 | e) IC 50 values obtained from cytotoxicity assays against Jurkat human ALLcells after incubating with free MTX, Gd-MTX, [email protected]/DOPE, and [email protected]/DOPE-AA. f) Save | Full Paper |
2020 | Fujita, H;Sasaki, T;Miyamoto, T;Akutsu, SN;Sato, S;Mori, T;Nakabayashi, K;Hata, K;Suzuki, H;Kosaki, K;Matsuura, S;Matsubara, Y;Amagai, M;Kubo, A; | Premature aging syndrome showing random chromosome number instabilities with CDC20 mutation | Aging cell | 33094908 | An age-matched normal control bone marrow mononuclear cell sample was obtained from AllCells. Cells were cultured in MethoCult H4034 Optimum (STEMCELL Technologies) at 1 × Save | Full Paper |
2020 | Yao, X;Ding, J; | Effects of Microstripe Geometry on Guided Cell Migration | ACS applied materials & interfaces | 32479054 | Primary rat mesenchymal stem cells (rMSCs) were purchased from Shanghai Allcells Tech. (China) and cultured in minimum essential medium α (MEM α, Gibco). Mouse embryo fibroblast cell line (NIH3T3) and human cervix epithelial carcinoma cell line (Hela | Full Paper |
2020 | Yang, H;Qing, K;Keeler, GD;Yin, L;Mietzsch, M;Ling, C;Hoffman, BE;Agbandje-McKenna, M;Tan, M;Wang, W;Srivastava, A; | Enhanced Transduction of Human Hematopoietic Stem Cells by AAV6 Vectors: Implications in Gene Therapy and Genome Editing | Molecular therapy. Nucleic acids | 32276210 | D, USA) supplemented with 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA) and 1% penicillin-streptomycin (Invitrogen, Grand Island, NY, USA). Human bone marrow CD34+ cells were purchased from AllCells (AllCells Technologies, Emeryville, CA, U | Full Paper |
2020 | Shin, JJ;Schröder, MS;Caiado, F;Wyman, SK;Bray, NL;Bordi, M;Dewitt, MA;Vu, JT;Kim, WT;Hockemeyer, D;Manz, MG;Corn, JE; | Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor Cells | Cell reports | 32877675 | RNA-sequencing datasets This paper BioProject ID PRJNA498122 ------------------------- Experimental Models ------------------------- Mobilized peripheral blood CD34+ Stem/Progenitor cells AllCells mPB015F NOD.Cg-PrkdcscidIl2rgtm1Wjl/Sz | Full Paper |
2020 | Nie, W;Zan, X;Yu, T;Ran, M;Hong, Z;He, Y;Yang, T;Ju, Y;Gao, X; | Synergetic therapy of glioma mediated by a dual delivery system loading α-mangostin and doxorubicin through cell cycle arrest and apoptotic pathways | Cell death & disease | 33116114 | animals The glioma cell lines C6, Gl261 and U87 and human umbilical vein endothelial cells (HUVECs) were purchased from the American Type Culture Collection (ATCC). Primary HUVECs were purchased from ALLCELLS (Shanghai, China). An EGM-2 BulletKit (Lo | Full Paper |
2020 | Zhang, XY;Rajagopalan, D;Chung, TH;Hooi, L;Toh, TB;Tian, JS;Rashid, MBMA;Sahib, NRBM;Gu, M;Lim, JJ;Wang, W;Chng, WJ;Jha, S;Chow, EK; | Frequent upregulation of G9a promotes RelB-dependent proliferation and survival in multiple myeloma | Experimental hematology & oncology | 32477831 | e), 1% Pen Strep (Invitrogen). For adherent cell lines, trypsin (Thermo Scientific, cat# 25200-056) was used to dissociate cells each passage. Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs, AllCells) were cultured in RPMI 1640 medium (BioWe | Full Paper |
2020 | Qian, Z;Fei, J;Zong, S;Yang, K;Li, L;Liu, R;Wang, Z;Cui, Y; | In Situ Visualization and SERS Monitoring of the Interaction between Tumor and Endothelial Cells Using 3D Microfluidic Networks | ACS sensors | 31885254 | Human umbilical vein endothelial cells (HUVEC) were purchased from Shanghai AllCells Biotech. Co., Ltd. Breast cancer cells (MCF7) were purchased from Nanjing KeyGen Biotech. Co., Ltd. The Roswell Park Memorial Institute (RMPI) 1640 cell culture medi | Full Paper |
2020 | Kim, YH;Park, GY;Rabinovitch, N;Tarafder, S;Lee, CH; | Effect of local anesthetics on viability and differentiation of various adult stem/progenitor cells | Stem cell research & therapy | 32894184 | udy that directly compared the effects of LAs across various stem/progenitor cells. Materials and methods Cell isolation Human bone marrow mesenchymal stem/progenitor cells (MSCs) were obtained from AllCells (Alameda, CA). With the Institutional Rev | Full Paper |
2020 | Xiong, MG;Xu, ZS;Li, YH;Wang, SY;Wang, YY;Ran, Y; | RNF152 positively regulates TLR/IL-1R signaling by enhancing MyD88 oligomerization | EMBO reports | 31930677 | HEK293T cells were purchased from ATCC. Human PBMCs were purchased from ALLCELLS. HFFs provided by Prof. Min‐Hua Luo (Wuhan Institute of Virology, CAS) were derived from human foreskins (male) 30. These cells were cultured in DMEM (HyClone) supplemen | Full Paper |
2020 | Han, Y;Gong, T;Zhang, C;Dissanayaka, WL; | HIF-1α Stabilization Enhances Angio-/Vasculogenic Properties of SHED | Journal of dental research | 32298193 | SHED were purchased from AllCells at passage 2 and characterized for mesenchymal origin. The results were published in our previous study (Xu et al. 2017). The cells were cultured in α-Modified Eagle’s Medium supplemented with 10% (v/v) fetal bovine | Full Paper |
2020 | Maser, IP;Hoves, S;Bayer, C;Heidkamp, G;Nimmerjahn, F;Eckmann, J;Ries, CH; | The Tumor Milieu Promotes Functional Human Tumor-Resident Plasmacytoid Dendritic Cells in Humanized Mouse Models | Frontiers in immunology | 33013879 | sulfan (Busilvex ,Pierre Faber) diluted in 0.9% Saline (Braun) was injected intraperitoneally (i.p.). Twenty-four hours later 105 human CD34+ umbilical cord blood-derived HSC (Stem Cell Technologies/Allcells) in 100 μl phosphate-buffered saline (PBS) | Full Paper |
2020 | Ghelani, A;Bates, D;Conner, K;Wu, MZ;Lu, J;Hu, YL;Li, CM;Chaudhry, A;Sohn, SJ; | Defining the Threshold IL-2 Signal Required for Induction of Selective Treg Cell Responses Using Engineered IL-2 Muteins | Frontiers in immunology | 32582190 | CD8+ T cells were purified from PBMCs isolated from healthy human donor derived leukopaks (AllCells) using EasySep CD8+ T cell Negative selection kit (STEMCELL Technologies) Save | Full Paper |
2020 | Shen, S;Sckisel, G;Sahoo, A;Lalani, A;Otter, DD;Pearson, J;DeVoss, J;Cheng, J;Casey, SC;Case, R;Yang, M;Low, R;Daris, M;Fan, B;Agrawal, NJ;Ali, K; | Engineered IL-21 Cytokine Muteins Fused to Anti-PD-1 Antibodies Can Improve CD8+ T Cell Function and Anti-tumor Immunity | Frontiers in immunology | 32457754 | Mismatched donor pair leukopaks were obtained from AllCells Inc., Donor's T cells were isolated using Pan T-cell Isolation Kit (Miltenyi Biotec, # 130-096-535) and a mismatched donor' Save | Full Paper |
2020 | Porcellini, S;Asperti, C;Corna, S;Cicoria, E;Valtolina, V;Stornaiuolo, A;Valentinis, B;Bordignon, C;Traversari, C; | CAR T Cells Redirected to CD44v6 Control Tumor Growth in Lung and Ovary Adenocarcinoma Bearing Mice | Frontiers in immunology | 32117253 | in of the ΔLNGFr (12). Generation of CD44v6 and CD19 CAR T Cells Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats or leukapheresis of healthy donors (San Raffaele Hospital; AllCells) by density gradient centrifugation (Lymph | Full Paper |
2020 | Baslan, T;Kendall, J;Volyanskyy, K;McNamara, K;Cox, H;D'Italia, S;Ambrosio, F;Riggs, M;Rodgers, L;Leotta, A;Song, J;Mao, Y;Wu, J;Shah, R;Gularte-Mérida, R;Chadalavada, K;Nanjangud, G;Varadan, V;Gordon, A;Curtis, C;Krasnitz, A;Dimitrova, N;Harris, L;Wigler, M;Hicks, J; | Novel insights into breast cancer copy number genetic heterogeneity revealed by single-cell genome sequencing | eLife | 32401198 | ta (i.e. ploidy) to achieve absolute copy number values. T-cell and B-cell sample processing and analysis Purified CD8+/CD4+ T-cells and CD19+/CD27+ memory B-cells purified cells were purchased from ALLCELLS (California, USA). Cells were thawed on i | Full Paper |
2020 | Münchow, EA;da Silva, AF;Piva, E;Cuevas-Suárez, CE;de Albuquerque, MTP;Pinal, R;Gregory, RL;Breschi, L;Bottino, MC; | Development of an antibacterial and anti-metalloproteinase dental adhesive for long-lasting resin composite restorations | Journal of materials chemistry. B | 33169763 | Human dental pulp stem cells (hDPSCs, AllCells, LLC, Alameda, CA, USA) obtained from permanent third molars were cultured in low glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Invitrogen Corporation, Grand Island, NY, USA) supplemented with | Full Paper |
2020 | Warnecke, A;Harre, J;Staecker, H;Prenzler, N;Strunk, D;Couillard-Despres, S;Romanelli, P;Hollerweger, J;Lassacher, T;Auer, D;Pachler, K;Wietzorrek, G;Köhl, U;Lenarz, T;Schallmoser, K;Laner-Plamberger, S;Falk, CS;Rohde, E;Gimona, M; | Extracellular vesicles from human multipotent stromal cells protect against hearing loss after noise trauma in vivo | Clinical and translational medicine | 33377658 | Human bone marrow (BM)-derived MSCs for the production of research-grade EV preparations were purchased from AllCells (Alameda, CA). Immunophenotype and viability analysis of Save | Full Paper |
2020 | Pinho, AC;Fonseca, AC;Caseiro, AR;Pedrosa, SS;Amorim, I;Branquinho, MV;Domingos, M;Maurício, AC;Santos, JD;Serra, AC;Coelho, JFJ; | Innovative tailor made dextran based membranes with excellent non-inflammatory response: In vivo assessment | Materials science & engineering. C, Materials for biological applications | 31761159 | Human dental pulp stem cells (hDPSCs) were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01). Minimum Essential Medium (MEM) α, GlutaMAX™ Supplement, no nucleosides, Streptomycin, Amphotericin B and HEPES buffer solution were purchase | Full Paper |
2020 | Wang, J;Ganaie, SS;Cheng, F;Xu, P;Ning, K;Wang, X;Kleiboeker, S;Cheng, S;Qiu, J; | RNA Binding Motif Protein RBM45 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19 through Binding to Novel Intron Splicing Enhancers | mBio | 32156816 | se conditions are dependent on RBM45. MATERIALS AND METHODS Ethics statement. Primary CD34+ hematopoietic stem cells were isolated from bone marrow of healthy human donors. We purchased the cells at AllCells (AllCells LLC, Alameda, CA). All of the c | Full Paper |
2020 | Dubrovsky, L;Ward, A;Choi, SH;Pushkarsky, T;Brichacek, B;Vanpouille, C;Adzhubei, AA;Mukhamedova, N;Sviridov, D;Margolis, L;Jones, RB;Miller, YI;Bukrinsky, M; | Inhibition of HIV Replication by Apolipoprotein A-I Binding Protein Targeting the Lipid Rafts | mBio | 31964734 | PBMCs from donors with the following HLA-B genotypes were purchased from AllCells Inc.: B*35 positive (B*15:17:01/B*35:01:01, B*35:01:01/B*55:01:01, B*35:01:01/B*35:01:01, B*35: Save | Full Paper |
2020 | Han, KH;Arlian, BM;Lin, CW;Jin, HY;Kang, GH;Lee, S;Lee, PC;Lerner, RA; | Agonist Antibody Converts Stem Cells into Migrating Brown Adipocyte-Like Cells in Heart | Cells | 31968623 | one, Chicago, IL, USA) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA). Expi293F cells were cultured in Expi293 Expression Media (Invitrogen, Carlsbad, CA, USA). Human CD34+ cells (AllCells, Alameda, CA, USA) were purchased and rep | Full Paper |
2020 | Zimran, E;Papa, L;Djedaini, M;Patel, A;Iancu-Rubin, C;Hoffman, R; | Expansion and preservation of the functional activity of adult hematopoietic stem cells cultured ex vivo with a histone deacetylase inhibitor | Stem cells translational medicine | 31950644 | Cryopreserved CD34+ cells from mPB or ABM from healthy adult donors were purchased from AllCells (Alameda, California) and stored in liquid nitrogen. The highly purified (90%-98% Save | Full Paper |
2020 | George, MJ;Prabhakara, K;Toledano-Furman, NE;Gill, BS;Wade, CE;Cotton, BA;Cap, AP;Olson, SD;Cox, CS; | Procoagulant in vitro effects of clinical cellular therapeutics in a severely injured trauma population | Stem cells translational medicine | 31903737 | Bone marrow mononuclear cells (BM MNCs) were isolated from fresh whole bone marrow from a commercial source (AllCells, Emeryville, California) according to common protocols Save | Full Paper |
2020 | Hou, L;Voit, RA;Sankaran, VG;Springer, TA;Yuki, K; | CD11c regulates hematopoietic stem and progenitor cells under stress | Blood advances | 33351105 | Human bone marrow cells and whole blood were purchased from AllCells (Quincy, MA). Lineage marker for human hematopoietic system includes: anti-hCD2 (RPA-2.10), anti-hCD3 ( Save | Full Paper |
2020 | Mascarenhas, JO;Rampal, RK;Kosiorek, HE;Bhave, R;Hexner, E;Wang, ES;Gerds, A;Abboud, CN;Kremyanskaya, M;Berenzon, D;Odenike, O;Farnoud, N;Krishnan, A;Weinberg, RS;McGovern, E;Salama, ME;Najfeld, V;Medina-Martinez, JS;Arango Ossa, JE;Levine, MF;Zhou, Y;Sandy, L;Heaney, ML;Levine, RL;Mesa, RA;Dueck, AC;Hoffman, R; | Phase 2 study of ruxolitinib and decitabine in patients with myeloproliferative neoplasm in accelerated and blast phase | Blood advances | 33104796 | Six control plasma samples (3 male, 3 female) collected from healthy donors between the ages of 30 and 50 years were procured from Allcells. Samples from 8 chronic-phase MF Save | Full Paper |
2020 | Marion, W;Boettcher, S;Ruiz-Torres, S;Lummertz da Rocha, E;Lundin, V;Morris, V;Chou, S;Zhao, AM;Kubaczka, C;Aumais, O;Zhang, Y;Shimamura, A;Schlaeger, TM;North, TE;Ebert, BL;Wells, SI;Daley, GQ;Rowe, RG; | An induced pluripotent stem cell model of Fanconi anemia reveals mechanisms of p53-driven progenitor cell differentiation | Blood advances | 33002135 | Institutional Review Board at Boston Children’s Hospital, after informed consent was obtained. This study was performed in accordance with the Declaration of Helsinki. Normal healthy BM mononuclear cells were purchased from AllCells | Full Paper |
2020 | Goldstein, RL;Goyos, A;Li, CM;Deegen, P;Bogner, P;Sternjak, A;Thomas, O;Klinger, M;Wahl, J;Friedrich, M;Rattel, B;Lamas, E;Min, X;Sudom, A;Farshbaf, M;Coxon, A;Balazs, M;Arvedson, T; | AMG 701 induces cytotoxicity of multiple myeloma cells and depletes plasma cells in cynomolgus monkeys | Blood advances | 32886754 | For staining of human PCs, human BM (AllCells) was stained with a fluorochrome-labeled antibody cocktail (supplemental Table 5). For analysis, doublets were gated out, and dead Save | Full Paper |
2020 | Walker, ZJ;VanWyngarden, MJ;Stevens, BM;Abbott, D;Hammes, A;Langouët-Astrie, C;Smith, CA;Palmer, BE;Forsberg, PA;Mark, TM;Jordan, CT;Sherbenou, DW; | Measurement of ex vivo resistance to proteasome inhibitors, IMiDs, and daratumumab during multiple myeloma progression | Blood advances | 32311014 | Normal donor samples were purchased from AllCells. For viability comparison with MM cells in MNC cultures, selection for CD138-positive cells was performed by using magnetic bead Save | Full Paper |
2020 | Romero-Masters, JC;Huebner, SM;Ohashi, M;Bristol, JA;Benner, BE;Barlow, EA;Turk, GL;Nelson, SE;Baiu, DC;Van Sciver, N;Ranheim, EA;Gumperz, J;Sherer, NM;Farrell, PJ;Johannsen, EC;Kenney, SC; | B cells infected with Type 2 Epstein-Barr virus (EBV) have increased NFATc1/NFATc2 activity and enhanced lytic gene expression in comparison to Type 1 EBV infection | PLoS pathogens | 32059024 | ized NSG mice Immunodeficient NSG (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson labs (catalogue number: 005557). Commercially available CD34-depleted human cord blood mononuclear cells (AllCells, LLC) were infected with various amounts o | Full Paper |
2020 | Shao, S;Qin, T;Qian, W;Li, X;Li, W;Han, L;Zhang, D;Wang, Z;Ma, Q;Wu, Z;Wu, E;Lei, J; | Cav-1 Ablation in Pancreatic Stellate Cells Promotes Pancreatic Cancer Growth through Nrf2-Induced shh Signaling | Oxidative medicine and cellular longevity | 32377291 | iated Hospital of Xi'an Jiaotong University, China. 2.3. Cell Lines Aspc-1 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and HUVECs were obtained from ALLCELLS (Emeryville, California, USA). In our exp | Full Paper |
2020 | Liu, H;Cheng, Q;Xu, DS;Wang, W;Fang, Z;Xue, DD;Zheng, Y;Chang, AH;Lei, YJ; | Overexpression of CXCR7 accelerates tumor growth and metastasis of lung cancer cells | Respiratory research | 33129326 | Human umbilical vein endothelial cells (HUVECs) and its completed medium were obtained from AllCells biological technology (Shanghai) co., LTD. And all these cell lines were Save | Full Paper |
2020 | Cheng, B;Ren, Y;Cao, H;Chen, J; | Discovery of novel resorcinol diphenyl ether-based PROTAC-like molecules as dual inhibitors and degraders of PD-L1 | European journal of medicinal chemistry | 32388281 | CD3 single chain variable fragment) and human PD-L1 (hPD-L1). Fresh PBMCs were purchased from ALLCELLS. Centrifuge at 350g for 10 min, discard the supernatant and resuspend in EasySep buffer at a density of 5 x107/mL. CD3+ T cells were isolated from | Full Paper |
2020 | Zhang, D;Qi, BY;Zhu, WW;Huang, X;Wang, XZ; | Crocin alleviates lipopolysaccharide-induced acute respiratory distress syndrome by protecting against glycocalyx damage and suppressing inflammatory signaling pathways | Inflammation research : official journal of the European Histamine Research Society ... [et al.] | 31925528 | HUVECs were cultured in complete culture medium (AllCells, Shanghai, China) in an incubator with a humidified atmosphere of 95% air and 5% CO 2 at 37 C. Cells at 80-90% Save | Full Paper |
2020 | Kuang, Z;Jing, H;Wu, Z;Wang, J;Li, Y;Ni, H;Zhang, P;Wu, W;Wu, M;Zhou, S;Qiu, X;Wu, D;Prinz, B;Baruah, H;Chen, B;Yu, M;Liu, J; | Development and characterization of a novel anti-OX40 antibody for potent immune activation | Cancer immunology, immunotherapy : CII | 32078015 | Two million LoVo cells suspended in 0.2 ml PBS were mixed with 0.66 million human peripheral blood mononuclear cells (PBMCs, ALLCELLS) and co-implanted subcutaneously in the Save | Full Paper |
2020 | Yao, Y;Yang, J;Qin, Q;Tang, C;Li, Z;Chen, L;Li, K;Ren, C;Chen, L;Rao, S; | Functional annotation of genetic associations by transcriptome-wide association analysis provides insights into neutrophil development regulation | Communications biology | 33340029 | Human CD34 + HSPCs from mobilized peripheral blood of deidentified healthy donors were purchased from AllCells. CD34 + HSPCs were cultured in StemSpan SFEM medium ( Save | Full Paper |
2020 | Mendes, LP;Rostamizadeh, K;Gollomp, K;Myerson, JW;Marcos-Contreras, OA;Zamora, M;Luther, E;Brenner, JS;Filipczak, N;Li, X;Torchilin, VP; | Monoclonal antibody 2C5 specifically targets neutrophil extracellular traps | mAbs | 33323006 | Whole blood was purchased from AllCells (AllCells utilizes standardized operating HIPAA compliance and approved protocols are followed by AllCells' collection facilities. The staff Save | Full Paper |
2020 | Zang, L;Song, Y;Yu, F;Liu, X; | Emodin relieved lipopolysaccharide-evoked inflammatory damage in WI-38 cells by up-regulating taurine up-regulated gene 1 | BioFactors (Oxford, England) | 31912578 | The human fibroblasts cell line WI-38 (AllCells, Shanghai, China) was hatched in a mixture of Dulbecco's modified Eagle's medium (DMEM; Gibco, Grand Island, NY), endothelial cell development supplement (Sigma, St. Louis, MO) (60 μg/ml), streptomycin | Full Paper |
2020 | Subhi, H;Husein, A;Mohamad, D;Nurul, A; | Physicochemical, mechanical and cytotoxicity evaluation of chitosan-based accelerated portland cement | Journal of Materials Research and Technology | Stem cells from human exfoliated deciduous teeth (SHED) was purchased from AllCells, USA. The cells were cultured in alpha minimum essential medium [Invitrogen, USA (GIBCO)] Save | Full Paper | |
2020 | Hu, S;Cavanagh, BL;Harrington, R;Ahmad, M;Kearns, G;Meaney, S;Wynne, C; | A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus Patients | International journal of molecular sciences | 33287382 | Microparticles are sub-micron, membrane-bound particles released from virtually allcells and which are present in the circulation. In several autoimmune disorders their amountand Save | Full Paper |
2020 | Wen, W;Song, S;Han, Y;Chen, H;Liu, X;Qian, Q; | An efficient Screening System in Yeast to Select a Hyperactive piggyBac Transposase for Mammalian Applications | International journal of molecular sciences | 32357554 | fied eagle medium (DMEM; Corning, Corning, NY, USA) supplemented with 10% fetal calf serum (FBS, Gibco, USA). The human peripheral blood mononuclear cells (PBMC) of healthy donors were purchased from AllCells (AllCells, Silicon Valley, CA, USA) under | Full Paper |
2020 | Damodharan, SN;Walker, KL;Forsberg, MH;McDowell, KA;Bouchlaka, MN;Drier, DA;Sondel, PM;DeSantes, KB;Capitini, CM; | Analysis of ex vivo expanded and activated clinical-grade human NK cells after cryopreservation | Cytotherapy | 32536506 | All cellular expansions occurred at the WBF under current Good Manufacturing Practice (GMP) quality system guidelines. Fresh leukopacks were commercially obtained (AllCells, Alameda, CA, USA, and Key Biologics, Memphis, TN, USA) from paid human apher | Full Paper |
2020 | Chen, Q;Jia, G;Zhao, X;Bao, Y;Zhang, Y;Ozkan, C;Minev, B;Ma, W; | Novel Survivin Peptides Screened With Computer Algorithm Induce Cytotoxic T Lymphocytes With Higher Cytotoxic Efficiency to Cancer Cells | Frontiers in molecular biosciences | 33102521 | PBMCs from five healthy donors were provided by San Diego Blood Bank and Scripps Green Hospital (San Diego, CA, United States). Normal human umbilical cord blood (UCB) CD34+ cells were purchased from AllCells (Alameda, CA, United States). This study | Full Paper |
2020 | Papa, L;Djedaini, M;Martin, TC;Zangui, M;Beaumont, KG;Sebra, R;Parsons, R;Schaniel, C;Hoffman, R; | Limited Mitochondrial Activity Coupled With Strong Expression of CD34, CD90 and EPCR Determines the Functional Fitness of ex vivo Expanded Human Hematopoietic Stem Cells | Frontiers in cell and developmental biology | 33384995 | Cryopreserved CD34 + cells from G-CSF mobilized peripheral blood (PB) or bone marrow (BM) aspirates harvested from healthy adult donors were purchased from AllCells. Save | Full Paper |
2020 | Yao, X;Xie, L;Zeng, Y; | MiR-9 Promotes Angiogenesis via Targeting on Sphingosine-1- Phosphate Receptor 1 | Frontiers in cell and developmental biology | 32850858 | and S1P3 in those normal ECs and tumor ECs were compared. The relationships between the significant differentially expressed miR-9 loci and specific S1P receptors were analyzed. Cell Culture HUVECs (Allcells, Shanghai, China) were cultured in HUVEC | Full Paper |
2020 | Juillerat, A;Tkach, D;Yang, M;Boyne, A;Valton, J;Poirot, L;Duchateau, P; | Straightforward Generation of Ultrapure Off-the-Shelf Allogeneic CAR-T Cells | Frontiers in bioengineering and biotechnology | 32671047 | ndependent T-cell donors. Significance is determined by a standard unpaired t-test, *p ≤ 0.05, **p ≤ 0.01. Materials and Methods T-Cell Proliferation Cryopreserved human PBMCs were acquired from ALLCELLS (cat #PB006F) and used in accordance with Cel | Full Paper |
2020 | Kuang, Z;Pu, P;Wu, M;Wu, Z;Wang, L;Li, Y;Zhang, S;Jing, H;Wu, W;Chen, B;Liu, J; | A Novel Bispecific Antibody with PD-L1-assisted OX40 Activation for Cancer Treatment | Molecular cancer therapeutics | 32999045 | Monocyte-derived immature dendrite cells (DC) isolated from human PBMCs (ALLCELLS) were maintained in AIM V medium CTS medium supplemented with GM-CSF (10 ng/mL, R&D Save | Full Paper |
2020 | Kotanides, H;Sattler, RM;Lebron, MB;Carpenito, C;Shen, J;Li, J;Surguladze, D;Haidar, JN;Burns, C;Shen, L;Inigo, I;Pennello, AL;Forest, A;Chen, X;Chin, D;Sonyi, A;Topper, M;Boucher, L;Sharma, P;Zhang, Y;Burtrum, D;Novosiadly, RD;Ludwig, DL;Plowman, GD;Kalos, M; | Characterization of 7A5: A Human CD137 (4-1BB) Receptor Binding Monoclonal Antibody with Differential Agonist Properties That Promotes Antitumor Immunity | Molecular cancer therapeutics | 32241872 | Human PBMCs were isolated from whole blood or leukopak obtained from NY Blood Center, AllCells, or Biological Specialty using Ficoll density gradient centrifugation (Ficoll-Paque Save | Full Paper |
2020 | Bian, W;Jing, X;Yang, Z;Shi, Z;Chen, R;Xu, A;Wang, N;Jiang, J;Yang, C;Zhang, D;Li, L;Wang, H;Wang, J;Sun, Y;Zhang, C; | Downregulation of LncRNA NORAD promotes Ox-LDL-induced vascular endothelial cell injury and atherosclerosis | Aging | 32267831 | (Shanghai, China). The C57BL/6 J mice fed with a normal diet were labeled as the WT control group. Cell culture, RNA interference and Adenovirus infection Primary HUVECs were obtained from Shanghai AllCells Biotech Co., Ltd. (Shanghai, China) and cu | Full Paper |
2020 | Karuna, R;Yokokawa, F;Wang, K;Zhang, J;Xu, H;Wang, G;Ding, M;Chan, WL;Abdul Ghafar, N;Leonardi, A;Seh, CC;Seah, PG;Liu, W;Srinivasa, RPS;Lim, SP;Lakshminarayana, SB;Growcott, E;Babu, S;Fenaux, M;Zhong, W;Gu, F;Shi, PY;Blasco, F;Chen, YL; | A Cyclic Phosphoramidate Prodrug of 2'-Deoxy-2'-Fluoro-2'-C-Methylguanosine for the Treatment of Dengue Virus Infection | Antimicrobial agents and chemotherapy | 32958712 | PBMCs (individual donors) were purchased from AllCells (Alameda, CA) or ReachBio (Seattle, WA). Written consent from the donors was available for all samples. All experiments involving human matrices were approved by the Institutional Review Board of | Full Paper |
2020 | Sebrow, J;Goff, SP;Griffin, DO; | Successfully transfected primary peripherally mobilized human CD34+ hematopoietic stem and progenitor cells (HSPCs) demonstrate increased susceptibility to retroviral infection | Virology journal | 32039735 | We obtained 293 T cells and Jurkat cells from ATCC, and peripherally mobilized CD34+ HSPCs from individual donors from AllCells (pre-isolated). 293 T cells were cultured in 20 mm X Save | Full Paper |
2020 | Zhong, C;Yu, Q;Jia, W;Yu, X;Yu, D;Yang, M;Wang, L;Ling, C;Zhu, L; | Mechanism for enhanced transduction of hematopoietic cells by recombinant adeno-associated virus serotype 6 vectors | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 32960474 | Primary human cord blood-derived CD34 + hematopoietic progenitors (mixed, 80% purity) were purchased from AllCells (Alameda, CA, USA) and cultured in C-IMDM in the presence of 1 ng/mL of recombinant human stem cell factor (rhSCF, BD Bioscience, San D | Full Paper |
2020 | Li, L;Wu, Z;Wu, M;Qiu, X;Wu, Y;Kuang, Z;Wang, L;Sun, T;Liu, Y;Yi, S;Jing, H;Zhou, S;Chen, B;Wu, D;Wu, W;Liu, J; | IBI112, a selective anti-IL23p19 monoclonal antibody, displays high efficacy in IL-23-induced psoriasiform dermatitis | International immunopharmacology | 33069927 | Mouse (C57BL/6, Beijing Vital River Laboratory Animal Technology) or cynomolgus monkey splenocytes (AllCells, USA) were cultured in the presence of IL-2 (R&D, USA). Different Save | Full Paper |
2020 | Kaneko, Y;Fukahori, H;Yamagami, K;Kawashima, T;Ito, M;Akamatsu, M;Marui, T;Kato, K;Takahashi, F;Morokata, T; | Effects of AS2819899, a novel selective PI3Kδ inhibitor, in a NZB/W F1 mouse lupus-like nephritis model | International immunopharmacology | 32736191 | Peripheral blood-derived frozen normal pan-T cells (AllCells, Alameda, CA, USA) were thawed according to the manufacturer’s instructions. The cell suspension (1 × 105 cells/well) was mixed with AS2819899 at designated concentrations in the anti-CD3 a | Full Paper |
2020 | Goydel, RS;Weber, J;Peng, H;Qi, J;Soden, J;Freeth, J;Park, H;Rader, C; | Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applications | The Journal of biological chemistry | 32193207 | Thr-245 and Ala-245, which arise from an SNP (rs10820900) in the frizzled domain of human ROR2 (hROR2-Fz). Human PBMCs were purchased from AllCells and cultured in X-VIVO 20 medium (Lonza) with 5% (v/v) off-the-clot human AB serum (Gemini Bio-Product | Full Paper |
2020 | Čuperlović-Culf, M;Khieu, NH;Surendra, A;Hewitt, M;Charlebois, C;Sandhu, JK; | Analysis and Simulation of Glioblastoma Cell Lines-Derived Extracellular Vesicles Metabolome | Metabolites | 32131411 | Normal human astrocytes (NHA, Lonza, Walkersville, MD, USA) were used as normal counterparts and were grown in astrocyte medium-containing supplements (Allcells) and 5% FBS. Save | Full Paper |
2020 | Liu, J;Kozhaya, L;Torres, VJ;Unutmaz, D;Lu, M; | Structure-based discovery of a small-molecule inhibitor of methicillin-resistant Staphylococcus aureus virulence | The Journal of biological chemistry | 32179646 | Blood samples were obtained from healthy, consenting donors as buffy coats (New York Blood Center) and leukopaks (AllCells, Alameda, CA). Human PBMCs were isolated from peripheral blood by density gradient centrifugation using Ficoll-Paque Plus (GE L | Full Paper |
2020 | Thwin, KKM;Ishida, T;Uemura, S;Yamamoto, N;Lin, KS;Tamura, A;Kozaki, A;Saito, A;Kishimoto, K;Mori, T;Hasegawa, D;Kosaka, Y;Nino, N;Takafuji, S;Iijima, K;Nishimura, N; | Level of Seven Neuroblastoma-Associated mRNAs Detected by Droplet Digital PCR Is Associated with Tumor Relapse/Regrowth of High-Risk Neuroblastoma Patients | The Journal of molecular diagnostics : JMD | 31837427 | 1 sample from BioChain (Newark, CA), 2 samples from STEMCELL (Vancouver, Canada), 56 samples from AllCells (Alameda, CA), 16 samples from ReachBio (Seattle, WA), and 28 samples from Loza (Walkersville, MD) (Supplemental Table S1). All PB samples from | Full Paper |
2020 | Xue, WL;Chen, RQ;Zhang, QQ;Li, XH;Cao, L;Li, MY;Li, Y;Lin, G;Chen, Y;Wang, MJ;Zhu, YC; | Hydrogen sulfide rescues high glucose-induced migration dysfunction in HUVECs by upregulating miR-126-3p | American journal of physiology. Cell physiology | 32186933 | Primary human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) (35). These HUVECs were cultured in endothelial complete medium (Allcells, Save | Full Paper |
2020 | Satani, N;Giridhar, K;Cai, C;Wewior, N;Norris, DD;Aronowski, J;Savitz, SI; | Medications for Hypertension Change the Secretome Profile from Marrow Stromal Cells and Peripheral Blood Monocytes | Stem cells international | 32802081 | MSCs were isolated from commercially available fresh human bone marrow aspirates (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously Save | Full Paper |
2020 | Diaz, MF;Horton, PD;Dumbali, SP;Kumar, A;Livingston, M;Skibber, MA;Mohammadalipour, A;Gill, BS;Zhang, S;Cox, CS;Wenzel, PL; | Bone marrow stromal cell therapy improves survival after radiation injury but does not restore endogenous hematopoiesis | Scientific reports | 33335275 | t received wet feed on days 4-30 post-exposure. Culture of human MSCs Human MSCs used for cell therapy were derived from whole bone marrow from independent human donors (commercially obtained from AllCells, LLC, Alameda, CA). Cells were isolated and | Full Paper |
2020 | Dzimianski, JV;Lorig-Roach, N;O'Rourke, SM;Alexander, DL;Kimmey, JM;DuBois, RM; | Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry | Scientific reports | 33303951 | dy at UCSC. All participants agreed to sample banking and future research use. The convalescent seropositive (SP) panel comprised 10 de-identified plasma samples from nine individuals, purchased from AllCells (Alameda, CA, USA). To be eligible for pl | Full Paper |
2020 | Diaz, MF;Horton, PD;Kumar, A;Livingston, M;Mohammadalipour, A;Xue, H;Skibber, MA;Ewere, A;Toledano Furman, NE;Aroom, KR;Zhang, S;Gill, BS;Cox, CS;Wenzel, PL; | Injury intensifies T cell mediated graft-versus-host disease in a humanized model of traumatic brain injury | Scientific reports | 32612177 | Bone marrow stromal cells were derived from whole bone marrow from independent human donors (AllCells, Alameda, CA). Mononuclear cells from whole bone marrow were enriched Save | Full Paper |
2020 | Allen, A;Vaninov, N;Li, M;Nguyen, S;Singh, M;Igo, P;Tilles, AW;O'Rourke, B;Miller, BLK;Parekkadan, B;Barcia, RN; | Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells | Scientific reports | 32576889 | ld times cover the range of expected delivery of the device from cell seeding location to clinical application. PBMC isolation and enrichment Peripheral blood mononuclear cells (PBMCs) obtained from AllCells LLC (AllCells, CA, USA) were freshly isol | Full Paper |
2020 | Siew Ching, H;Thirumulu Ponnuraj, K;Luddin, N;Ab Rahman, I;Nik Abdul Ghani, NR; | Early Odontogenic Differentiation of Dental Pulp Stem Cells Treated with Nanohydroxyapatite-Silica-Glass Ionomer Cement | Polymers | 32957636 | the present study was principally aimed at evaluating the effect of nanoHA-silica-GIC on the differentiation of DPSCs into odontogenic lineage. 2. Materials and Methods 2.1. Cell Culture DPSCs (AllCells, Alameda, CA, USA; Cat no. DP003F) were grown | Full Paper |
2020 | Keskar, V;Sood, A;Loghin, E;Kovacs, E;Duthie, RS;Liu, S;Park, JH;Chadwick, C;Smith, R;Brown, M;Stroncek, DF;Highfill, SL; | Novel DNA-based T-Cell Activator Promotes Rapid T-Cell Activation and Expansion | Journal of immunotherapy (Hagerstown, Md. : 1997) | 32796275 | Activation studies were conducted in 6-well tissue culture plastic plates using commercially available Pan T cells (AllCells, Alameda, CA). Cultures were diluted as needed (1:3 or 1:4) to Save | Full Paper |
2020 | Shrestha, B;Zhang, Y;Yu, B;Li, G;Boucher, JC;Beatty, NJ;Tsai, HC;Wang, X;Mishra, A;Sweet, K;Lancet, JE;Kelley, L;Davila, ML; | Generation of Antitumor T Cells For Adoptive Cell Therapy With Artificial Antigen Presenting Cells | Journal of immunotherapy (Hagerstown, Md. : 1997) | 31834208 | To investigate the ability of aAPCs to support T-cell expansion we isolated T cells from healthy donor PBMCs (ALLcells) using a Human T-cell isolation Kit (Stem Cells Inc., Cambridge, Save | Full Paper |
2020 | Park, LM;Lannigan, J;Jaimes, MC; | OMIP-069: Forty-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood | Cytometry. Part A : the journal of the International Society for Analytical Cytology | 32830910 | All human PBMCs used in this study were obtained from AllCells Alameda. Ethical review and regulatory compliance were conducted by Alpha Independent Review Board under Save | Full Paper |
2020 | Nair, RR;Piktel, D;Hathaway, QA;Rellick, SL;Thomas, P;Saralkar, P;Martin, KH;Geldenhuys, WJ;Hollander, JM;Gibson, LF; | Pyrvinium Pamoate Use in a B cell Acute Lymphoblastic Leukemia Model of the Bone Tumor Microenvironment | Pharmaceutical research | 31989336 | Primary CD3+ T cells, peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) were purchased from AllCells (Allcells.com) and maintained in Lymphocyte Growth Medium-3 (Lonza, Cat No: CC-3211) containing 10% FBS and 1x strep | Full Paper |
2020 | Powell, AB;Ren, Y;Korom, M;Saunders, D;Hanley, PJ;Goldstein, H;Nixon, DF;Bollard, CM;Lynch, RM;Jones, RB;Cruz, CRY; | Engineered Antigen-Specific T Cells Secreting Broadly Neutralizing Antibodies: Combining Innate and Adaptive Immune Response against HIV | Molecular therapy. Methods & clinical development | 33005704 | Peripheral blood samples were obtained from deidentified, healthy donor buffy coats from the NIH, through the Department of Transfusion Medicine, or commercially from AllCells. Save;erved. Peripheral Blood Samples Peripheral blood samples were obt | Full Paper |
2020 | Fang, Y;Zhang, Y;Guo, C;Chen, C;Gao, H;Zhou, X;Liu, T;Qian, Q; | Safety and Efficacy of an Immune Cell-Specific Chimeric Promoter in Regulating Anti-PD-1 Antibody Expression in CAR T Cells | Molecular therapy. Methods & clinical development | 32995356 | PBMCs were purchased from AllCells (USA) using a protocol approved by the Ethics Committee of the Chinese Peoples' Liberation Army General Hospital (S2019-063-01). In total, 5 × Save;Cell Isolation, Electroporation, and Ex Vivo CAR T Cell Expansion | Full Paper |
2020 | Shapiro, J;Iancu, O;Jacobi, AM;McNeill, MS;Turk, R;Rettig, GR;Amit, I;Tovin-Recht, A;Yakhini, Z;Behlke, MA;Hendel, A; | Increasing CRISPR Efficiency and Measuring Its Specificity in HSPCs Using a Clinically Relevant System | Molecular therapy. Methods & clinical development | 32478125 | (5 μL total volume). Complexes were allowed to form for 10-20 min at room temperature before electroporation. Genome Editing by RNP Electroporation in CD34+ HSPCs Mobilized human CD34+ HSPCs (AllCells, Alameda, CA, USA) were thawed and cultured for | Full Paper |
2020 | Morgan, RA;Ma, F;Unti, MJ;Brown, D;Ayoub, PG;Tam, C;Lathrop, L;Aleshe, B;Kurita, R;Nakamura, Y;Senadheera, S;Wong, RL;Hollis, RP;Pellegrini, M;Kohn, DB; | Creating New β-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer Sequences | Molecular therapy. Methods & clinical development | 32426415 | BM CD34+ Cell Culture and Transduction All BM aspirates were obtained from voluntary healthy donors supplied by AllCells (Alameda, CA, USA). BM mononuclear cells were isolated Save | Full Paper |
2020 | Mehler, VJ;Burns, CJ;Stauss, H;Francis, RJ;Moore, ML; | Human iPSC-Derived Neural Crest Stem Cells Exhibit Low Immunogenicity | Molecular therapy. Methods & clinical development | 32055644 | utamine, 2 mM L-glutamine, 50 U/mL penicillin/streptomycin, 20 ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF), and 20 ng/mL IL-4. BM-MSCs were derived from bone marrow aspirates (AllCells, Alameda, CA, USA) in-house as previously des | Full Paper |
2020 | Elnaggar, M;Al-Mohannadi, A;Kizhakayil, D;Raynaud, CM;Al-Mannai, S;Gentilcore, G;Pavlovski, I;Sathappan, A;Van Panhuys, N;Borsotti, C;Follenzi, A;Grivel, JC;Deola, S; | Flow-Cytometry Platform for Intracellular Detection of FVIII in Blood Cells: A New Tool to Assess Gene Therapy Efficiency for Hemophilia A | Molecular therapy. Methods & clinical development | 31886317 | ar endothelial cells) and U937 (pre-monocytic cells) cell lines were kindly provided by Antonia Follenzi (UPO, Italy). HeLa (cervical cancer cells) were purchased from ATCC. PBMCs were purchased from AllCells (Alameda, CA, USA). Cells were cultured i | Full Paper |
2020 | Ning, K;Wang, MJ;Lin, G;Zhang, YL;Li, MY;Yang, BF;Chen, Y;Huang, Y;Li, ZM;Huang, YJ;Zhu, L;Liang, K;Yu, B;Zhu, YZ;Zhu, YC; | eNOS-Nitric Oxide System Contributes to a Novel Antiatherogenic Effect of Leonurine via Inflammation Inhibition and Plaque Stabilization | The Journal of pharmacology and experimental therapeutics | 32238453 | Primary human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (H-001F-C; China) and cultured in HUVEC Growth Medium (H-004; Allcells), and 100 U/ml of Save | Full Paper |
2020 | Zhaorigetu, S;Bair, H;Jin, D;Gupta, VS;Pandit, LM;Bryan, RM;Lally, KP;Olson, SD;Cox, CS;Harting, MT; | Extracellular Vesicles Attenuate Nitrofen-Mediated Human Pulmonary Artery Endothelial Dysfunction: Implications for Congenital Diaphragmatic Hernia | Stem cells and development | 32475301 | Human MSCs were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using density centrifugation and plastic Save | Full Paper |
2020 | Baumgartner, JM;Riviere, P;Lanman, RB;Kelly, KJ;Veerapong, J;Lowy, AM;Kurzrock, R; | Prognostic Utility of Pre- and Postoperative Circulating Tumor DNA Liquid Biopsies in Patients with Peritoneal Metastases | Annals of surgical oncology | 32767050 | Background Circulating tumor DNA (ctDNA) is a promising technology for treatment selection, prognostication, and surveillance after definitive therapy. Its use in the perioperative Save | Full Paper |
2020 | Hamburger, AE;DiAndreth, B;Cui, J;Daris, ME;Munguia, ML;Deshmukh, K;Mock, JY;Asuelime, GE;Lim, ED;Kreke, MR;Tokatlian, T;Kamb, A; | Engineered T cells directed at tumors with defined allelic loss | Molecular immunology | 33012527 | Leukopaks were purchased from AllCells®. Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB), with strict oversight. HIPAA compliance and approved protocols were also followed. Frozen PBMCs were thawed | Full Paper |
2020 | Xu, H;Hamburger, AE;Mock, JY;Wang, X;Martin, AD;Tokatlian, T;Oh, J;Daris, ME;Negri, KR;Gabrelow, GB;Wu, ML;Nampe, DP;Asuelime, GE;McElvain, ME;Sandberg, ML;Kamb, A; | Structure-function relationships of chimeric antigen receptors in acute T cell responses to antigen | Molecular immunology | 32768859 | Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols Save | Full Paper |
2020 | Wang, Z;Zhou, G;Risu, N;Fu, J;Zou, Y;Tang, J;Li, L;Liu, H;Liu, Q;Zhu, X; | Lenalidomide Enhances CAR-T Cell Activity Against Solid Tumor Cells | Cell transplantation | 32967454 | line U251 CD133-OE luc. Similarly, the tumor cell line U251 WT luc, MDA-MB-453 luc, and MDA-MB-468 luc were also obtained by nucleofection. Peripheral blood mononuclear cells (PBMCs) were bought from ALLCELLS (PB005F, Alameda, CA, USA). Following the | Full Paper |
2020 | Zhao, C;Zhao, Z;Wang, Z;Hu, L;Wang, H;Fang, Z; | Supervillin promotes tumor angiogenesis in liver cancer | Oncology reports | 32468064 | Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells LLC (cat. H-004; AllCells LLC) in a 0.25% gelatin-coated culture flask. HepG2, Bel7405 and MHCC- Save | Full Paper |
2020 | Lee, KS;Lee, J;Lee, P;Kim, CU;Kim, DJ;Jeong, YJ;Park, YJ;Tesh, VL;Lee, MS; | Exosomes released from Shiga toxin 2a-treated human macrophages modulate inflammatory responses and induce cell death in toxin receptor expressing human cells | Cellular microbiology | 32772454 | Human CD14 + monocytes were purchased from AllCells LLC and maintained in RPMI 1640 medium containing 10% FBS, 5.0 μg/ml streptomycin and 5.0 U/ml penicillin. Cells (1.0 × Save | Full Paper |
2020 | Monroe, MN;Zhaorigetu, S;Gupta, VS;Jin, D;Givan, KD;Curylo, AL;Olson, SD;Cox, CS;Segura, A;Buja, LM;Grande-Allen, KJ;Harting, MT; | Extracellular vesicles influence the pulmonary arterial extracellular matrix in congenital diaphragmatic hernia | Pediatric pulmonology | 32568428 | Human mesenchymal stromal cells (hMSCs) were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using Save | Full Paper |
2020 | Zaw, SYM;Kaneko, T;Zaw, ZCT;Sone, PP;Murano, H;Gu, B;Okada, Y;Han, P;Katsube, KI;Okiji, T; | Crosstalk between dental pulp stem cells and endothelial cells augments angiogenic factor expression | Oral diseases | 32248596 | Stem cells from human exfoliated deciduous teeth (SHEDs; AllCells) were cultured with a stem cell growth medium (Dulbecco's modified Eagle's medium (DMEM)/F12; Gibco) containing 15% fetal bovine serum (Gibco-BRL, Grand Island, NY), 10,000 units penic | Full Paper |
2020 | Huang, GL;Nampe, DP;Yi, J;Gabrelow, GB;Negri, KR;Kamb, A;Xu, H; | A multivariate, quantitative assay that disentangles key kinetic parameters of primary human T cell function in vitro | PloS one | 33166305 | Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols Save | Full Paper |
2020 | Wang, X;Chacon, LI;Derakhshandeh, R;Rodriguez, HJ;Han, DD;Kostyushev, DS;Henry, TD;Traverse, JH;Moyé, L;Simari, RD;Taylor, DA;Springer, ML; | Impaired therapeutic efficacy of bone marrow cells from post-myocardial infarction patients in the TIME and LateTIME clinical trials | PloS one | 32841277 | Healthy de-identified donor BM MNCs were purchased from AllCells (Alameda, CA). These MNCs were manually isolated at AllCells using Ficoll conditions like that in the Sepax Save | Full Paper |
2020 | Schönherz, AA;Bødker, JS;Schmitz, A;Brøndum, RF;Jakobsen, LH;Roug, AS;Severinsen, MT;El-Galaly, TC;Jensen, P;Johnsen, HE;Bøgsted, M;Dybkær, K; | Normal myeloid progenitor cell subset-associated gene signatures for acute myeloid leukaemia subtyping with prognostic impact | PloS one | 32324791 | [18] GSE42519, [19] GSE19599, [20] GSE17054, [21] and GSE19429 [22] cohorts. For GSE63270, [18] and GSE17054, [21] human bone marrow mononuclear cells (BMMCs) from healthy donors were purchased from ALLCELLS (Emeryville, CA) where collection protocol | Full Paper |
2020 | Tani, J;Ito, Y;Tatemichi, S;Yamakami, M;Fukui, T;Hatano, Y;Kakimoto, S;Kotani, A;Sugimura, A;Mihara, K;Yamamoto, R;Tanaka, N;Minami, K;Takahashi, K;Hirato, T; | Physicochemical and biological evaluation of JR-131 as a biosimilar to a long-acting erythropoiesis-stimulating agent darbepoetin alfa | PloS one | 32302352 | fects of the test substances on colony-forming unit-erythroid (CFU-E)- and burst-forming unit-erythroid (BFU-E)-derived colony formation in human bone-marrow-derived mononuclear cells (purchased from AllCells, CA) were examined. The cells were obtain | Full Paper |
2020 | Steinberg, L; | The Effects of P. Gingivalis Ceramide Lipids on Platelet Size, Agglutination, Aggregation, and Vascular Surface Adhesion Markers | Thesis | Background: Increased bleeding on probing has been proven to be correlated with the progression of periodontal disease. Gingival bleeding in sites with periodontitis has often been attributed to chronic inflammation. Previous research has documented | Full Paper | |
2020 | Kim, DH;Kim, DH;Heck, BE;Shaffer, M;Yoo, KH;Hur, J; | PPAR-δ agonist affects adipo-chondrogenic differentiation of human mesenchymal stem cells through the expression of PPAR-γ | Regenerative therapy | 33426208 | In this experimental study, frozen human bone marrow mononuclear cells were used for isolation (AllCells, Alameda, CA, USA) [25]. Cells were resuspended in α-minimum essential Save;mation condition and its correlation with PPAR-δ. 2 Methods 2.1 Is | Full Paper |
2020 | Zhang, E;Xie, L;Qin, P;Lu, L;Xu, Y;Gao, W;Wang, L;Xie, MH;Jiang, W;Liu, S; | Quality by Design-Based Assessment for Analytical Similarity of Adalimumab Biosimilar HLX03 to Humira® | The AAPS journal | 32385732 | PMBC cells (Allcells) were incubated with mAbs with a 2-fold serial dilution for 30 min followed by TNFα-Biotin (Sinobio) incubation. After labeled by Streptavidin-PE (eBioscience), Save | Full Paper |
2020 | Dai, R;Heid, B;Xu, X;Xie, H;Reilly, CM;Ahmed, SA; | EGR2 is elevated and positively regulates inflammatory IFNγ production in lupus CD4+ T cells | BMC immunology | 32646370 | ethods Human peripheral blood mononuclear cells (PBMCs) The PBMCs of human patients of lupus (n = 4, all female) and healthy controls (n = 4, 2 male and 2 female) were purchased directly from AllCells LLC (Alameda, CA, USA). Based on the information | Full Paper |
2020 | Dong, W;Zhang, X;Lu, Z; | Effect of 532nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor in cultured human vascular endothelial cells | Photodiagnosis and photodynamic therapy | 32344194 | Working drug solutions (0-8 μg/ml) were prepared by diluting the stock solution with RPMI-1640 medium (AllCells). A 532 nm neodymium-doped yttrium orthovanadate (Nd:YVO 4 ) Save | Full Paper |
2020 | Pieles, O;Reck, A;Reichert, TE;Morsczeck, C; | p53 inhibits the osteogenic differentiation but does not induce senescence in human dental follicle cells | Differentiation; research in biological diversity | 32473528 | Human dental follicle cells (DFCs) were obtained from AllCells. DFCs were cultivated as described previously (Morsczeck et al., 2016). Cells were analyzed before and after the Save | Full Paper |
2020 | Lafleur, MA;Werner, J;Fort, M;Lobenhofer, EK;Balazs, M;Goyos, A; | MRGPRX2 activation as a rapid, high-throughput mechanistic-based approach for detecting peptide-mediated human mast cell degranulation liabilities | Journal of immunotoxicology | 32525431 | Human mobilized peripheral blood CD34+ cells (derived from healthy donors treated with G-CSF to mobilize CD34+ progenitor cells from the bone marrow to the peripheral blood, then isolated using anti-CD34 magnetic beads) (#mPB016F, AllCells, Alameda, | Full Paper |
2020 | Yu, Z;Yin, S;Zhang, W;Jiang, X;Hu, J; | Picosecond laser texturing on titanium alloy for biomedical implants in cell proliferation and vascularization | Journal of biomedical materials research. Part B, Applied biomaterials | 31692202 | Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCells Biological Technology Limited company and cultured at 1 × 105 on plates with Dulbecco's Modified Eagle's medium supplemented with 10% fetal bovine serum, 100 U/ml streptomyc | Full Paper |
2020 | Bhargav, A;Min, KS;Wen Feng, L;Fuh, JYH;Rosa, V; | Taguchi's methods to optimize the properties and bioactivity of 3D printed polycaprolactone/mineral trioxide aggregate scaffold: Theoretical predictions and experimental validation | Journal of biomedical materials research. Part B, Applied biomaterials | 31112004 | The use of human dental pulp stem cells (DPSC) from single donor (DPF003, Allcells, Alameda) was approved by NUS Institutional Review Board (Approval Number: NUS 2094). The characterization and expressions of CD34, CD73, CD90, CD105 are available els | Full Paper |
2020 | Al-Amoodi, AS;Sakashita, K;Ali, AJ;Zhou, R;Lee, JM;Tehseen, M;Li, M;Belmonte, JCI;Kusakabe, T;Merzaban, JS; | Using Eukaryotic Expression Systems to Generate Human α1,3-Fucosyltransferases That Effectively Create Selectin-Binding Glycans on Stem Cells | Biochemistry | 32901486 | leukemia cell line), THP1 (human acute monocytic leukemia), HL60 (human acute promyelocytic leukemia) (ATCC), and MSCs (primary mesenchymal stromal cells) (AllCells) were Save | Full Paper |
2020 | Behbehani, GK;Finck, R;Samusik, N;Sridhar, K;Fantl, WJ;Greenberg, PL;Nolan, GP; | Profiling myelodysplastic syndromes by mass cytometry demonstrates abnormal progenitor cell phenotype and differentiation | Cytometry. Part B, Clinical cytometry | 31917512 | in Table S2, with the risk‐based clinical status of MDS patients indicated by IPSS category and morphologic characteristics (Greenberg et al., 1997). Five healthy control samples were obtained from Allcells, Inc. using the same protocol. Normal bone | Full Paper |
2020 | Kim, DH;Kim, DH;Heck, BE;Shaffer, M;Hur, J;Yoo, KH; | A natural supplement formula reduces anti-oxidative stress and enhances osteo-chondrogenic differentiation potential in mesenchymal stem cells | Journal of clinical biochemistry and nutrition | 32523247 | rozen mononuclear cells from human bone marrow were purchased (AllCells, Emeryville, CA) and used to isolate human bone marrow-mesenchymal stem cells (hBM-MSCs). Briefly, thawed mononuclear cells were cultured in Dulbecco’s modified Eagle medium (DME | Full Paper |
2020 | Wang, P;Qin, W;Liu, T;Jiang, D;Cui, L;Liu, X;Fang, Y;Tang, X;Jin, H;Qian, Q; | PiggyBac-engineered T cells expressing a glypican-3-specific chimeric antigen receptor show potent activities against hepatocellular carcinoma | Immunobiology | 31522780 | Human peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from AllCells (AllCells, Silicon, USA). 5 × 106−1 × 107 PBMCs were electroporated with 6 μg of GPC3CAR plasmid or an equal quantity of MOCK plasmid in an electroporat | Full Paper |
2020 | Liu, H;Gemmell, L;Lin, R;Zuo, F;Balfour, HH;Woo, JC;Hayes, GM; | Development of an Improved Epstein-Barr Virus (EBV) Neutralizing Antibody Assay to Facilitate Development of a Prophylactic gp350-Subunit EBV Vaccine | Mediterranean journal of hematology and infectious diseases | 32180911 | Healthy human donor serum samples were purchased from AllCells, LLC and Bioreclamation, LLC (n=39). Samples were heated at 56 oC for 30 minutes to inactivate complement, and Save | Full Paper |
2020 | Chien, KS;Class, CA;Montalban-Bravo, G;Wei, Y;Sasaki, K;Naqvi, K;Ganan-Gomez, I;Yang, H;Soltysiak, KA;Kanagal-Shamanna, R;Do, KA;Kantarjian, HM;Garcia-Manero, G; | LILRB4 expression in chronic myelomonocytic leukemia and myelodysplastic syndrome based on response to hypomethylating agents | Leukemia & lymphoma | 32036728 | BM samples from healthy individuals were obtained from AllCells (Emeryville, CA, USA). CD34+ cells were isolated using the CD34 MicroBead Kit (Miltenyi Biotec, Bergisch Gladback, Save | Full Paper |
2020 | Wu, T; | The mean ± 1 SD was calculated for triplicate cultures. The laboratory (ReachBio LLC, Seattle, Washington) was blinded to the identify of the test compounds and docetaxel served as the positive control, with blank and vehicle as negative controls. | Thesis | The surviving mice of the “young adult” group at day 35 post-TBI were sacrificed and 5×104 BM cells acquired from femurs were plated in ColonyGEL Mouse Complete Medium (Reach Bio, Seattle, WA) as previously described [131, 135]. After 7 days of incub | Full Paper | |
2020 | White, CM;Haidekker, MA;Kisaalita, WS; | Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D Cultures | SLAS technology | 31997709 | Human bone marrow derived (Tulane Center for Gene Therapy) 3.2 ± 1.4 AFM indentation Spread morphology on glass coverslips; cytoplasm measured; spherical AFM tip 76 Human bone marrow derived (AllCells) 33 ± 7 AFM indentation Spread morphology on Ther | Full Paper |
2020 | Chen, Y; | NOVEL SIGNALS IN STRESS ERYTHROPOIESIS: COORDINATED CONTROL OF WNT, PGE2 AND PERK SIGNALING PATHWAYS REGULATES EXPANSION AND DIFFERENTIATION OF STRESS ERYTHROID PROGENITORS | Thesis | Human bone marrow mononuclear cells (MNCs) (ReachBio) were thawed at 37°C water according to the ReachBio instructions. MNCs were enumerated and cell concentrations were adjusted to 5 105 cells/mL. MNCs were cultured in Gibco RPMI-1640 (Invitrogen | Full Paper | |
2020 | Wu, JL;McIntyre, PW;Hong, JM;Yassen, GH;Bruzzaniti, A; | Effects of radiopaque double antibiotic pastes on the proliferation, alkaline phosphatase activity and mineral deposition of dental pulp stem cells | Archives of oral biology | 32485262 | Human DPSC were purchased from ALLCells (Alameda, CA, USA), passaged once and aliquots stored in liquid nitrogen. For each experiment, DPSC were thawed and seeded into 10-cm2 dishes and cultured in α-MEM (HyClone Laboratories Inc., South Logan, UT, U | Full Paper |
2020 | Madanagopal, TT;Franco-Obregón, A;Rosa, V; | Comparative study of xeno-free induction protocols for neural differentiation of human dental pulp stem cells in vitro | Archives of oral biology | 31600663 | The DPSC were isolated from the third molar from single donor (DPF003, AllCells, USA). Due to ethical requirements, the age, gender and ethnic group of the donor cannot be disclosed Save | Full Paper |
2020 | Pieles, O;Reck, A;Morsczeck, C; | High endogenous expression of parathyroid hormone-related protein (PTHrP) supports osteogenic differentiation in human dental follicle cells | Histochemistry and cell biology | 32710187 | Human dental follicle cells (DFCs), which were purchased from AllCells, were cultivated in DMEM (Dulbecco's modified Eagle medium) supplemented with 10% fetal bovine serum and Save | Full Paper |
2020 | Fujishiro, A;Iwasa, M;Fujii, S;Maekawa, T;Andoh, A;Tohyama, K;Takaori-Kondo, A;Miura, Y; | Menatetrenone facilitates hematopoietic cell generation in a manner that is dependent on human bone marrow mesenchymal stromal/stem cells | International journal of hematology | 32572826 | Normal human BM samples were purchased from AllCells (Alameda, CA). To confirm consistency among donors, multiple batches of BM-MSCs were isolated as described previously [ Save | Full Paper |
2020 | Yu, CI;Marches, F;Wu, TC;Martinek, J;Palucka, K; | Techniques for the generation of humanized mouse models for immuno-oncology | Methods in enzymology | 32178826 | Adult CD34 + HPCs from G-CSF mobilized peripheral blood or bone marrow may be obtained from Allcells (Alameda, CA, USA) or Lonza (Basel, Switzerland). Cord blood CD34 + Save | Full Paper |
2020 | Enezei, H;Qabbani, A;Ahmad, A;Khamis, M;Hassani, A;Hamad, H; | The Effect of Strontium on Osteoblastogenesis and Osteoclastogenesis in Dental Stem Cells-induced Epidermal Growth Factor at Molecular Level: In Vitro Study | Journal of Hard Tissue Biology | AllCells, LLC Company, Emeryville, CA, USA Dental Stem Cells (DSCs) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Dulbecco’s modified Eagles medium (DMEM) high glucose(4.5g/l) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Fetal bovine ser | Full Paper | |
2020 | Kuang, Z;Li, L;Zhang, P;Chen, B;Wu, M;Ni, H;Yi, S;Zou, J;Liu, J; | A novel antibody targeting TIM-3 resulting in receptor internalization for cancer immunotherapy | Antibody therapeutics | 33928230 | The antibody was purified in-house (Innovent Biologics Co., Ltd., Suzhou, China) from HEK293 cells with either transient or stable expression unless indicated otherwise. Cell culture Human PBMCs (AllCells, Alameda, CA, USA) were cultured in AIM V M | Full Paper |
2020 | Moussi, K;Abusamra, DB;Yassine, O;Merzaban, J;Kosel, J; | Strain-induced Differentiation of Mesenchymal Stem Cells | Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference | 33018453 | Human bone marrow derived mesenchymal stem/stromal cells (hMSCs) were purchased from AllCells. All the experiments were performed using MSCs in their 3-5th passage. An Save | Full Paper |
2020 | Yang, Z;Wang, T;Wu, D;Min, Z;Tan, J;Yu, B; | RNA N6-methyladenosine reader IGF2BP3 regulates cell cycle and angiogenesis in colon cancer | Journal of experimental & clinical cancer research : CR | 32993738 | lls were cultured in RPMI 1640 medium supplemented with 10% FBS (Invitrogen, Carlsbad, CA, USA) at 37 °C in a 5% CO2 atmosphere. Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured in End | Full Paper |
2020 | MacGlashan, D; | Modulating the Human Basophil Phenotype During Its Development and Maturation: Basophils Derived from In Vitro Cultures of CD34+ Progenitor Cells | Methods in molecular biology (Clifton, N.J.) | 32766967 | CD34+ progenitor stems (commercially prepared, multiple sources, e.g., AllCells (Quincy, MA, USA), or Fred Hutchinson Cancer Research Center (Seattle, WA, USA)). 12. FBS (fetal bovine serum) (multiple sources, heat-inactivated). 13. Penicillin/stre | Full Paper |
2020 | Urkasemsin, G;Rungarunlert, S;Ferreira, JN; | Bioprinting Strategies for Secretory Epithelial Organoids | Methods in molecular biology (Clifton, N.J.) | 32207117 | hDPSC lines are commercially available through AllCells (Alameda, CA, USA) and should be used at lower passage to guarantee that their proliferation rate and phenotype are stable. 2. The same lot of FBS should be utilized during the all experiments. | Full Paper |
2020 | Han, SS;Kang, SW; | Metabolic Labeling of Live Stem Cell for In Vitro Imaging and In Vivo Tracking | Methods in molecular biology (Clifton, N.J.) | 30997638 | Stem cell therapy offers promising solutions to diseases and injuries that traditional medicines and therapies can't effectively cure. To get and explain their full therapeutic potentials, the Save | Full Paper |
2020 | Al-Mohannadi, A;Deola, S;Malki, A; | Visualization of Factor Viii with Flow-Cytometry as a tool for Novel Gene Therapy Approach in Hemophilia A | University of the Future: Re-Imagining Research and Higher Education | received as a kind gift from Antonia Follenzi, Health Sciences Department in Piemonte Orientale University in Novara-Italy. HELA cells (cervical cancer) were purchased from American Type Culture Collection (ATCC™), USA. PBMCs purchased by AllCells (C | Full Paper | |
2020 | Romito, M;Juillerat, A;Kok, Y;HIldenbeutel, M;Rhiel, M;Andrieux, G;Geiger, J;Rudolph, C;Mussolino, C;Duclert, A;Metzner, K;Duchateau, P;Cathomen, T;Cornu, T; | Clinically relevant CCR5 editing in primary CD4+ T cells with TALEN confers resistance to HIV-1 infection | Authorea, Inc. | For oligonucleotide capture assay (OCA), human PBMCs (ALLCELLS, USA) were plated at a density of 1x106 cells/ml in X-vivo-15 media (Lonza, Switzerland) supplemented with 5% Save | Full Paper | |
2020 | Turchiano, G;Andrieux, G;Blattner, G;Pennucci, V;Klermund, J;Monaco, G;Poddar, S;Mussolino, C;Cornu, T;Boerries, M;Cathomen, T; | Quantitative Evaluation of Chromosomal Rearrangements in Primary Gene-Edited Human Stem Cells by Preclinical CAST-Seq | SSRN Electronic Journal | Cell culture and transfection. Cryopreserved human CD34+ HSPCs derived from cord blood (AllCells, Cat.# CB008F; StemCell-Technologies, Cat.# 70008.3) were thawed and cultivated in a density range of 0.5-1 x 106 /ml at 37°C, 5% CO2 in GMP-grade CellG | Full Paper | |
2020 | Lu, B;Yang, H;Liu, F;Sun, Y;He, H;Ding, X;Hu, S;xia, l; | Escherichia coli Nissle 1917 secreting functional interleukin 2 targets tumours and enhances the immune response to suppress tumours | Research Square | CT26 colon carcinoma lines were maintained by our laboratory, and peripheral blood mononuclear cells (PBMCs) were purchased from Allcells Biotechnology (Shanghai) Co., Ltd. Save | Full Paper | |
2020 | Zhang, Y;Wang, P;wang, t;Fang, Y;Ding, Y;Qian, Q; | Chimeric Antigen Receptor T Cells Engineered to Secrete CD40 Agonist Antibodies Enhance Antitumor Efficacy | Research Square | Human peripheral blood mononuclear cells (PBMC) from healthy donors were purchased from AllCells (Shanghai, China) and cryopreserved in our laboratory. Save | Full Paper | |
2020 | Mangiras, D;Mattheakis, P;Ribet, P;Dimitrakopoulos, G; | Soft-Clustering Driven Flip-flop Placement Targeting Clock-induced OCV | Proceedings of the 2020 International Symposium on Physical Design | In this way, the cluster initialization of clock net n is done on AllCells that includes both the cells connected to n and the pre-defined cluster centers of the hierarchically lower clock sub- Save | Full Paper | |
2020 | Li, W;Yang, S;Xu, P;Zhang, D;Tong, Y;Chen, L;Jia, B;Li, A;Ru, D;Zhang, B;Liu, M;Lian, C;Chen, C;Fu, W;Yuan, S;Ren, X;Liang, Y;Yang, Z;Li, W;Wang, S;Zhang, X;Lu, H;Xu, J;Wang, H;Yu, W; | Human Identical Sequences of SARS-CoV-2 Promote Clinical Progression of COVID-19 by Upregulating Hyaluronan via NamiRNA-Enhancer Network | bioRxiv | HUVEC (human umbilical vein endothelial cell, bought from AllCells) was cultured in the commercial culture medium (AllCells, H-004) according to the manufacturer's guidelines. Save | Full Paper | |
2020 | Fiskin, E;Lareau, C;Eraslan, G;Ludwig, L;Regev, A; | Single-cell multimodal profiling of proteins and chromatin accessibility using PHAGE-ATAC | bioRxiv | For PBMC and CD8 T cell experiments, cryopreserved PBMCs or CD8 T cells (AllCells) were thawed, washed in PBS and resuspended in cold Flow cytometry buffer (FC buffer; PBS containing 2% FBS). All centrifugation steps were carried out at 350g, 4min, 4 | Full Paper | |
2020 | Mimitou, E;Lareau, C;Chen, K;Zorzetto-Fernandes, A;Takeshima, Y;Luo, W;Huang, T;Yeung, B;Thakore, P;Wing, J;Nazor, K;Sakaguchi, S;Ludwig, L;Sankaran, V;Regev, A;Smibert, P; | Scalable, multimodal profiling of chromatin accessibility and protein levels in single cells | bioRxiv | Cryopreserved healthy donor peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BM) were obtained from AllCells (USA) or Cellular Technology Limited (CTL) and processed immediately after thawing. NIH-3T3 and HEK293FT cells were maintain | Full Paper | |
2020 | Steinbuck, M;Seenappa, L;Jakubowski, A;McNeil, L;Haqq, C;DeMuth, P; | A Lymph Node Targeted Amphiphile Vaccine Induces Potent Cellular and Humoral Immunity to SARS-CoV-2 | bioRxiv | SARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and ALLCELLS (Alameda, CA), respectively. All samples were received and stored frozen at -80°C until analysis | Full Paper | |
2020 | Keating, S;Mizrahi, R;Adams, M;Asensio, M;Benzie, E;Carter, K;Chiang, Y;Edgar, R;Gautam, B;Gras, A;Leong, J;Leong, R;Lim, Y;Manickam, V;Medina-Cucurella, A;Niedecken, A;Saini, J;Simons, J;Spindler, M;Stadtmiller, K;Tinsley, B;Wagner, E;Wayham, N;Tracy, L;Lundberg, C;Büscher, D;Terencio, J;Roalfe, L;Pearce, E;Richardson, H;Goldblatt, D;Ramjag, A;Carrington, C;Simmons, G;Muench, M;Chamow, S;Monroe, B;Olson, C;Oguin, T;Lynch, H;Jeanfreau, R;Meyer, E;Adler, A;Johnson, D; | Capturing and Recreating Diverse Antibody Repertoires as Multivalent Recombinant Polyclonal Antibody Drugs | bioRxiv | Plasma-derived polyclonal antibodies are polyvalent drugs used for many important clinical indications that require modulation of multiple drug targets simultaneously, including Save | Full Paper | |
2020 | Sharma, R;Dever, D;Lee, C;Azizi, A;Pan, Y;Camarena, J;Köhnke, T;Bao, G;Porteus, M;Majeti, R; | TRACE-Seq Reveals Clonal Reconstitution Dynamics of Gene Targeted Human Hematopoietic Stem Cells | bioRxiv | For in vivo studies presented, cord blood-derived CD34 + cells were purchased from AllCells or Stemcell Technologies and were thawed according to the manufacturer's Save | Full Paper | |
2020 | Miller, I;Sun, L;Harris, A;Gamboa, L;Zamat, A;Kwong, G; | Remote control of CAR T cell therapies by thermal targeting | bioRxiv | Primary Human CD3+ cells were obtained from an anonymous donor blood after apheresis (AllCells) and were cryopreserved in 90% FBS and 10% DMSO until subsequent use. After Save | Full Paper | |
2020 | Mandal, S;Sunagawa, S;Prathipati, P;Belshan, M;Shibata, A;Destache, C; | Targeted immuno-antiretroviral HIV therapeutic approach to provide dual protection and boosts cellular immunity: A proof-of-concept study | bioRxiv | Human immunodeficiency virus (HIV)-infected active and latent CCR5 expressing long-lived T-cells are the primary barrier to HIV/AIDS eradication. Broadly neutralizing antibodies and Save | Full Paper | |
2020 | Burley, D;Campagna, C;Harte, A;Kelly, S;Spiegelhoff, M; | Effects of Erythrocyte Aggregation on Blood Rheology in Regard to Future Sepsis Diagnosis Application | Thesis | The preparation of the blood dextran solutions for the micro-ESR, capillary fill, and vibration syllectometry were all done as one big batch. Blood was obtained from AllCells, LLC, a reputable blood and marrow cell supplier from Quincy, Massachusetts | Full Paper | |
2020 | Fleischer, LC; | Engineering Novel Chimeric Antigen Receptors (CARs) for T-cell Malignancies Using Innate Immune Cells | Thesis | Methods and Materials γδ T-cell expansion. gd T cells were expanded from cryopreserved PBMCs purchased from AllCells (Alameda, California) or from fresh PBMCs isolated from 30-50 mL consented, healthy adult blood. PBMCs were isolated from blood using | Full Paper | |
2020 | O'Brien, G; | Genetic and epigenetic consequences of radiation exposure in human and mouse leukaemogenesis | Thesis | Greece with informed consent from each patient. Bone marrow aspirates were obtained from 5 normal donors (ALLCELLS, Alameda, CA) with informed consent and ethical approval from the Alpha Independent Review Board. Bone marrow aspirates from 7 AML pati | Full Paper | |
2020 | Kimmey, SC; | Mapping Single Cell Protein & Biosynthesis Dynamics Across Human Embryonic & Adult Stem Cell Specification | Thesis | Fresh bone marrow aspirates from two independent donors were obtained from AllCells (Alameda, CA) on the same day of collection, and immediately after receiving was placed in 37C 5% CO2 incubator for 30 minutes prior to SOM3B labeling. A mixture of a | Full Paper | |
2020 | Davis-Marcisak, E;Fitzgerald, A;Kessler, M;Danilova, L;Jaffee, E;Zaidi, N;Weiner, L;Fertig, E; | Transfer learning between preclinical models and human tumors identifies conserved NK cell activation signature in anti-CTLA-4 responsive tumors | bioRxiv | Fresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selected from donor peripheral blood using CD56 positivity. Donor NK cell purity Save | Full Paper | |
2020 | Burgenson, D; | Development of a Cell-Free Protein Expression System Derived from Human Blood Cells | Thesis | A comparison between methods used to produce leukapheresis products purchased from Allcells and the New York Blood Center. From the experiments performed, the Allcells Save | Full Paper | |
2020 | Yun, ZX; | Identification of G9a as a Therapeutically Targetable Driver of Multiple Myeloma Tumorigenesis | Thesis | dissociate cells each passage. Cryopreserved Peripheral Blood Mononuclear Cells (PBMC, AllCells) were cultured in RPMI 1640 medium (BioWest, Kansas City, MO) with supplement of 10% FBS (HyClone) and 1% penicillin/streptomycin (Invitrogen) one day bef | Full Paper | |
2020 | Lindgren, A;Mohidin, D; | Applying Digital Marketing Methods in the Healthcare Industry: A Case Study at Immuneed | Thesis | Immuneed Volume Immuneed Traffic Celentyx Volume Celentyx Traffic Reachbio Volume Reachbio Traffic Eurofins Volume Eurofins Traffic Immundnz Volume Immundnz Traffic Nexelis Volume Nexelis Traffic ImmuneCarta Volume ImmuneCarta Traffic | Full Paper | |
2020 | Su, R;Dong, L;Li, Y;Gao, M;Han, L;Wunderlich, M;Deng, X;Li, H;Huang, Y;Gao, L;Li, C;Zhao, Z;Robinson, S;Tan, B;Qing, Y;Qin, X;Prince, E;Xie, J;Qin, H;Li, W;Shen, C;Sun, J;Kulkarni, P;Weng, H;Huang, H;Chen, Z;Zhang, B;Wu, X;Olsen, MJ;Müschen, M;Marcucci, G;Salgia, R;Li, L;Fathi, AT;Li, Z;Mulloy, JC;Wei, M;Horne, D;Chen, J; | Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune Evasion | Cancer cell | 32531268 | After 7 days of selection with 0.5mg/ml G418 Sulfate (10131027, Thermo Fisher Scientific) in ColonyGEL (1201, ReachBio Research Lab), the cells were collected and injected into lethally irradiated (960 rads) 8- to 10-week-old B6.SJL (CD45.1, RRID: IM | Full Paper |
2020 | Young, TM;Reyes, C;Pasnikowski, E;Castanaro, C;Wong, C;Decker, CE;Chiu, J;Song, H;Wei, Y;Bai, Y;Zambrowicz, B;Thurston, G;Daly, C; | Autophagy protects tumors from T cell-mediated cytotoxicity via inhibition of TNFα-induced apoptosis | Science immunology | 33443027 | For human tumor cell killing experiments using CD3 bispecific antibody, human T cells were isolated from peripheral blood mononuclear cells (PBMCs) (ReachBio) using Dynabeads Untouched Human T Cells Kit (Thermo Fisher Scientific) | Full Paper |
2020 | Shen, C;Sheng, Y;Zhu, AC;Robinson, S;Jiang, X;Dong, L;Chen, H;Su, R;Yin, Z;Li, W;Deng, X;Chen, Y;Hu, YC;Weng, H;Huang, H;Prince, E;Cogle, CR;Sun, M;Zhang, B;Chen, CW;Marcucci, G;He, C;Qian, Z;Chen, J; | RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid Leukemia | Cell stem cell | 32402250 | Thereafter, the transduced cells were then plated into ColonyGEL methylcellulose medium (ReachBio, Seattle, WA) supplied with 10 ng/ml of murine recombinant IL-3, IL-6, GM-CSF and 30 ng/ml of murine recombinant SCF, along with 1.0 mg/ml of G418 (Gibc | Full Paper |
2020 | Chiu, D;Tavaré, R;Haber, L;Aina, OH;Vazzana, K;Ram, P;Danton, M;Finney, J;Jalal, S;Krueger, P;Giurleo, JT;Ma, D;Smith, E;Thurston, G;Kirshner, JR;Crawford, A; | A PSMA-Targeting CD3 Bispecific Antibody Induces Antitumor Responses that Are Enhanced by 4-1BB Costimulation | Cancer immunology research | 32184296 | PBMCs were obtained from ReachBio (WA) for in vivo (ReachBio). C4-2 tumor cells were subcutaneously injected with 5 × 10 6 tumor cells and 1 × 10 6 human PBMCs (ReachBio). Save | Full Paper |
2020 | Patterson, AM;Liu, L;Sampson, CH;Plett, PA;Li, H;Singh, P;Mohammad, KS;Hoggatt, J;Capitano, ML;Orschell, CM;Pelus, LM; | A Single Radioprotective Dose of Prostaglandin E2 Blocks Irradiation-Induced Apoptotic Signaling and Early Cycling of Hematopoietic Stem Cells | Stem cell reports | 32735825 | CFCs were quantitated as described by Hoggatt et al. (2013a). TNCs from non-IR mice were plated at 2 × 104 per 35-mm dish in triplicate in ColonyGEL Mouse Complete Medium (Reach Bio, Seattle, WA). Cells from IR mice were plated at 0.5-1 × 106 per dis | Full Paper |
2020 | Paulson, RF;Hariharan, S;Little, JA; | Stress erythropoiesis: definitions and models for its study | Experimental hematology | 32750404 | Control PBMCs were purchased from ReachBio. PBMCs obtained were plated at 1 x 10 6 cells/ml stress erythropoiesis differentiation media containing human growth factors. The cells Save | Full Paper |
2020 | Patterson, AM;Plett, PA;Chua, HL;Sampson, CH;Fisher, A;Feng, H;Unthank, JL;Miller, SJ;Katz, BP;MacVittie, TJ;Orschell, CM; | Development of a Model of the Acute and Delayed Effects of High Dose Radiation Exposure in Jackson Diversity Outbred Mice; Comparison to Inbred C57BL/6 Mice | Health physics | 32932286 | Colony-forming unit cell (CFU-C) analyses Functional BM progenitor number was analyzed utilizing methylcellulose colony assays as per manufacturer instructions (Colony Gel 1202, ReachBio, Seattle, WA) | Full Paper |
2020 | Radtke, S;Colonna, L;Perez, AM;Hoffman, M;Kean, LS;Kiem, HP; | Isolation of a Highly Purified HSC-enriched CD34+CD90+CD45RA- Cell Subset for Allogeneic Transplantation in the Nonhuman Primate Large-animal Model | Transplantation direct | 33134503 | CFC) assays were sorted in purity mode to prevent crosscontamination by different subsets. Colony-forming Cell Assay For CFC assays, 1000 to 1200 sorted cells were seeded into 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA). Hematopoietic colonies wer | Full Paper |
2019 | Bonner, M;Kanter, J;Macari, E;Lane, R;Lewis, G;Coles, P;Kassenaar, S;Mynampati, S;Schulze, R;Hebert, M;Walters, M;Thompson, A;Asmal, M;Tisdale, J;Pierciey, F; | The Relationships between Target Gene Transduction, Engraftment of HSCs and RBC Physiology in Sickle Cell Disease Gene Therapy | Blood | Background LentiGlobin for Sickle Cell Disease (SCD) gene therapy contains ex-vivo lentiviral vector (LVV)-mediated addition of a modified β-globin gene (βA-T87Q) into autologous CD34+ hematopoietic stem cells (HSCs). The safety and ef | Full Paper | |
2019 | Kanter, J;Tisdale, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Schmidt, M;Miller, A;Pierciey, F;Huang, W;Ribeil, J;Thompson, A;Walters, M; | Resolution of Sickle Cell Disease Manifestations in Patients Treated with Lentiglobin Gene Therapy: Updated Results from the Phase 1/2 Hgb-206 Group C Study | Blood | Background β-globin gene transfer into hematopoietic stem cells (HSCs) could reduce or eliminate sickle cell disease (SCD)-related manifestations. LentiGlobin for SCD gene therapy contains autologous CD34+ cells transduced with the BB3 | Full Paper | |
2019 | Walters, M;Tisdale, J;Kwiatkowski, J;Krishnamurti, L;Mapara, M;Schmidt, M;Miller, A;Pierciey, F;Huang, W;Ribeil, J;Kanter, J;Thompson, A; | Exploring the Drivers of Potential Clinical Benefit in Initial Patients Treated in the Hgb-206 Study of Lentiglobin for Sickle Cell Disease (SCD) Gene Therapy | Blood | Background LentiGlobin for SCD gene therapy contains autologous CD34+ hematopoietic stem cells (HSCs) transduced with the BB305 lentiviral vector (LVV) encoding β-globin with an anti-sickling substitution (T87Q). Its safety and efficac | Full Paper | |
2019 | Eapen, M;Neuberg, D;Mendizabal, A;Stevenson, K;Antin, J;DiFronzo, N;El Rassi, F;Lulla, P;Waller, E;Garrison, J;Smith, S;Sullivan, K;Walters, M;Krishnamurti, L; | A Phase II Trial to Compare Allogeneic Transplant Vs. Standard of Care for Severe Sickle Cell Disease: Blood and Marrow Transplant Clinical Trials Network (BMT CTN) Protocol 1503 | Blood | Background: Allogeneic hematopoietic cell transplantation (HCT) is a curative treatment for sickle cell disease (SCD). A pilot trial confirmed the suitability of a myeloablative conditioning regimen (busulfan/fludarabine/r-ATG) for HLA-matched siblin | Full Paper | |
2019 | Loi, S;Giobbie-Hurder, A;Gombos, A;Bachelot, T;Hui, R;Curigliano, G;Campone, M;Biganzoli, L;Bonnefoi, H;Jerusalem, G;Bartsch, R;Rabaglio-Poretti, M;Kammler, R;Maibach, R;Smyth, M;Di Leo, A;Colleoni, M;Viale, G;Regan, M;André, F;Fumagalli, D;Gelber, R;Goulioti, T;Hiltbrunner, A;Hui, R;Roschitzki, H;Ruepp, B;Boyle, F;Stahel, R;Aebi, S;Coates, A;Goldhirsch, A;Karlsson, P;Kössler, I;Fournarakou, S;Gasca, A;Pfister, R;Ribeli-Hofmann, S;Weber, M;Celotto, D;Comune, C;Frapolli, M;Sánchez-Hohl, M;Huang, H;Mahoney, C;Price, K;Scott, K;Shaw, H;Fischer, S;Greco, M;King, C;Andrighetto, S;Piccart-Gebhart, M;Findlay, H;Jenkins, M;Karantza, V;Mejia, J;Schneier, P; | Pembrolizumab plus trastuzumab in trastuzumab-resistant, advanced, HER2-positive breast cancer (PANACEA): a single-arm, multicentre, phase 1b–2 trial | The Lancet Oncology | PD-L1 status for the first 79 patients was assessed with the QualTek immunohistochemistry assay (QualtTek Molecular Laboratories, Santa Barbara, CA, USA) in which PD-L1 staining in at least 1% of tumour cells or any staining in stroma were defined as | Full Paper | |
2019 | Kassim, A;Walters, M;Eapen, M;Allison, B;Mendizabal, A;Brodsky, R;DeBaun, M; | Reduced Intensity Conditioning for Haploidentical Bone Marrow Transplantation in Patients with Symptomatic Sickle Cell Disease: BMT CTN Protocol 1507 | Blood | Background: Hematopoietic cell transplantation (HCT) has curative potential in sickle cell disease (SCD). A reduced intensity haploidentical bone marrow transplant (haplo-BMT) platform piloted by investigators at Johns Hopkins, addressed key barriers | Full Paper | |
2019 | Nghiem, P;Bhatia, S;Lipson, EJ;Sharfman, WH;Kudchadkar, RR;Brohl, AS;Friedlander, PA;Daud, A;Kluger, HM;Reddy, SA;Boulmay, BC;Riker, AI;Burgess, MA;Hanks, BA;Olencki, T;Margolin, K;Lundgren, LM;Soni, A;Ramchurren, N;Church, C;Park, SY;Shinohara, MM;Salim, B;Taube, JM;Bird, SR;Ibrahim, N;Fling, SP;Homet Moreno, B;Sharon, E;Cheever, MA;Topalian, SL; | Durable Tumor Regression and Overall Survival in Patients With Advanced Merkel Cell Carcinoma Receiving Pembrolizumab as First-Line Therapy | J. Clin. Oncol. | 30726175 | 21,22. PD-L1 IHC. PD-L1 staining (anti-PD-L1 clone 22C3; Merck Research Laboratories, Kenilworth, NJ) was performed at QualTek Molecular Laboratories (Newtown, PA) on formalin-fixed paraffin-embedded sections from pretreatment MCC biopsies, as previo | Full Paper |
2019 | Fedoriw, A;Rajapurkar, SR;O'Brien, S;Gerhart, SV;Mitchell, LH;Adams, ND;Rioux, N;Lingaraj, T;Ribich, SA;Pappalardi, MB;Shah, N;Laraio, J;Liu, Y;Butticello, M;Carpenter, CL;Creasy, C;Korenchuk, S;McCabe, MT;McHugh, CF;Nagarajan, R;Wagner, C;Zappacosta, F;Annan, R;Concha, NO;Thomas, RA;Hart, TK;Smith, JJ;Copeland, RA;Moyer, MP;Campbell, J;Stickland, K;Mills, J;Jacques-O'Hagan, S;Allain, C;Johnston, D;Raimondi, A;Porter Scott, M;Waters, N;Swinger, K;Boriack-Sjodin, A;Riera, T;Shapiro, G;Chesworth, R;Prinjha, RK;Kruger, RG;Barbash, O;Mohammad, HP; | Anti-tumor Activity of the Type I PRMT Inhibitor, GSK3368715, Synergizes with PRMT5 Inhibition through MTAP Loss | Cancer Cell | 31257072 | GSK3368715 was evaluated at 20, 5, 1.25, 0.3125 and 0.078 mM in a total of 10 patient samples. DLBCL patient cells from 10 unique donors were received as frozen samples from Conversant Bio. The human biological samples were sourced ethically and thei | Full Paper |
2019 | Leighl, NB;Hellmann, MD;Hui, R;Carcereny, E;Felip, E;Ahn, MJ;Eder, JP;Balmanoukian, AS;Aggarwal, C;Horn, L;Patnaik, A;Gubens, M;Ramalingam, SS;Lubiniecki, GM;Zhang, J;Piperdi, B;Garon, EB; | Pembrolizumab in patients with advanced non-small-cell lung cancer (KEYNOTE-001): 3-year results from an open-label, phase 1 study | Lancet Respir Med | 30876831 | NR=not reached. PD-L1=programmed death ligand 1. TPS=tumour proportion score. *PD-L1 TPS 1% on the basis of the QualTek assay (Molecular Laboratories, Goleta | Full Paper |
2019 | Melamed, Z;López-Erauskin, J;Baughn, MW;Zhang, O;Drenner, K;Sun, Y;Freyermuth, F;McMahon, MA;Beccari, MS;Artates, JW;Ohkubo, T;Rodriguez, M;Lin, N;Wu, D;Bennett, CF;Rigo, F;Da Cruz, S;Ravits, J;Lagier-Tourenne, C;Cleveland, DW; | Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegeneration | Nat. Neurosci. | 30643298 | ... titute for the kind contribution of the SIN18 vector. We thank B. Ren for providing the Illumina sequencing platform. We thank A. Goginashvili for valued input on the manuscript. We thank J. Nuovo at Phylogeny Inc. (Columbus, OH) for performing t | Full Paper |
2019 | Thompson, A;Walters, M;Kwiatkowski, J;Hongeng, S;Porter, J;Sauer, M;Thrasher, A;Thuret, I;Elliot, H;Tao, G;Colvin, R;Locatelli, F; | Northstar-2: Updated Safety and Efficacy Analysis of Lentiglobin Gene Therapy in Patients with Transfusion-Dependent β-Thalassemia and Non-β0/β0 Genotypes | Blood | Background Transfusion-dependent β-thalassemia (TDT) is treated with regular, lifelong red blood cell (RBC) transfusions and despite iron-chelating therapy, carries a risk of serious organ damage from iron overload and other complicati | Full Paper | |
2019 | Petermann, F;Pękowska, A;Johnson, CA;Jankovic, D;Shih, HY;Jiang, K;Hudson, WH;Brooks, SR;Sun, HW;Villarino, AV;Yao, C;Singleton, K;Akondy, RS;Kanno, Y;Sher, A;Casellas, R;Ahmed, R;O'Shea, JJ; | The Magnitude of IFN-γ Responses Is Fine-Tuned by DNA Architecture and the Non-coding Transcript of Ifng-as1 | Mol. Cell | For LCMV experiments RNA was isolated from sorted cells of LCMV-infected mice with the QIAGEN AllPrep Micro Kit. Libraries were prepared and sequenced by the HudsonAlpha Genomic Services Lab (paired-end, 150 bp reads). For all other experiments total | Full Paper | |
2019 | Kwiatkowski, J;Thompson, A;Rasko, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;Schmidt, M;Kletzel, M;Vichinsky, E;Deary, B;Chen, Y;Petrusich, A;Walters, M; | Long-Term Clinical Outcomes of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia in the Northstar (HGB-204) Study | Blood | Background Patients with transfusion-dependent β-thalassemia (TDT) may experience transfusional iron overload and end-organ damage. While potentially curative, allogeneic hematopoietic stem cell (HSC) transplantation is limited by tran | Full Paper | |
2019 | Furie, R;Werth, VP;Merola, JF;Stevenson, L;Reynolds, TL;Naik, H;Wang, W;Christmann, R;Gardet, A;Pellerin, A;Hamann, S;Auluck, P;Barbey, C;Gulati, P;Rabah, D;Franchimont, N; | Monoclonal antibody targeting BDCA2 ameliorates skin lesions in systemic lupus erythematosus | J. Clin. Invest. | 30645203 | ... un-exposed skin of HVs matched approximately by age and ethnicity to the BIIB059-treated cohort (mean age = 34; range, 28–37; White, 6/8; Asian, 1/8; Black or African American, 1/8; female, 8/8) by Folio Biosciences, then processed into paraffin | Full Paper |
2019 | Hudson, WH;Prokhnevska, N;Gensheimer, J;Akondy, R;McGuire, DJ;Ahmed, R;Kissick, HT; | Expression of novel long noncoding RNAs defines virus-specific effector and memory CD8+ T cells | Nat Commun | 30643116 | ... of Emory University. RNA Isolation and sequencing RNA was isolated from sorted cells of LCMV-infected mice with the Qiagen AllPrep Micro Kit. Library preparation and sequencing were performed by the HudsonAlpha Genomic Services Laboratory via th | Full Paper |
2019 | Ma, X;Shao, Y;Tian, L;Flasch, DA;Mulder, HL;Edmonson, MN;Liu, Y;Chen, X;Newman, S;Nakitandwe, J;Li, Y;Li, B;Shen, S;Wang, Z;Shurtleff, S;Robison, LL;Levy, S;Easton, J;Zhang, J; | Analysis of error profiles in deep next-generation sequencing data | Genome Biol. | 30867008 | Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA. Xiaotu.Ma@stjude.org.++Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.++Department of Computatio | Full Paper |
2019 | Zeng, Q;Liao, C;Terhune, J;Wang, L; | Impacts of florfenicol on the microbiota landscape and resistome as revealed by metagenomic analysis | Microbiome | 31818316 | Carlsbad, CA) according to the manufacturer's instructions. The library construction and sequencing were conducted at the HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). Genomic libraries were prepared with the | Full Paper |
2019 | Lee, CR;Yonk, AJ;Wiskerke, J;Paradiso, KG;Tepper, JM;Margolis, DJ; | Opposing Influence of Sensory and Motor Cortical Input on Striatal Circuitry and Choice Behavior | Curr. Biol. | 30982651 | Light was delivered to the cortical afferents and PV interneurons in the striatum through an optical fiber patchcord (Thorlabs; length 1 m; 0.50 nA; 200 mm diameter) connected to an optical cannula (described above) via a mating sleeve (Thorlabs). A | Full Paper |
2019 | Ramachandran, I;Lowther, DE;Dryer-Minnerly, R;Wang, R;Fayngerts, S;Nunez, D;Betts, G;Bath, N;Tipping, AJ;Melchiori, L;Navenot, JM;Glod, J;Mackall, CL;D'Angelo, SP;Araujo, DM;Chow, WA;Demetri, GD;Druta, M;Van Tine, BA;Grupp, SA;Abdul Razak, AR;Wilky, B;Iyengar, M;Trivedi, T;Winkle, EV;Chagin, K;Amado, R;Binder, GK;Basu, S; | Systemic and local immunity following adoptive transfer of NY-ESO-1 SPEAR T cells in synovial sarcoma | J Immunother Cancer | 31651363 | d Cross (Philadelphia, PA). NY-ESO-1 testing was performed via IHC at a Clinical Laboratory Improvement Amendments certified pathology laboratory at the National Cancer Institute (Bethesda, MD) or at QualTek Labs (Goleta, CA). Disease response was cl | Full Paper |
2019 | Spino, M;Kurz, SC;Chiriboga, L;Serrano, J;Zeck, B;Sen, N;Patel, S;Shen, G;Vasudevaraja, V;Tsirigos, A;Suryadevara, CM;Frenster, JD;Tateishi, K;Wakimoto, H;Jain, R;Riina, HA;Nicolaides, TP;Sulman, EP;Cahill, DP;Golfinos, JG;Isse, K;Saunders, LR;Zagzag, D;Placantonakis, DG;Snuderl, M;Chi, AS; | Cell Surface Notch Ligand DLL3 is a Therapeutic Target in Isocitrate Dehydrogenase-mutant Glioma | Clin. Cancer Res. | 30397180 | For the discovery set, 20 nontumor brain tissue samples and 63 glioma tumor samples were obtained from Cooperative Human Tumor Network (CHTN), Conversant Bio, and RS Diagnostics. This set of tumors included the following diagnoses: glioblastoma, WHO | Full Paper |
2019 | Habra, MA;Stephen, B;Campbell, M;Hess, K;Tapia, C;Xu, M;Rodon Ahnert, J;Jimenez, C;Lee, JE;Perrier, ND;Boraddus, RR;Pant, S;Subbiah, V;Hong, DS;Zarifa, A;Fu, S;Karp, DD;Meric-Bernstam, F;Naing, A; | Phase II clinical trial of pembrolizumab efficacy and safety in advanced adrenocortical carcinoma | J Immunother Cancer | 31533818 | PD-L1 staining was performed by Qualtek using Merck 22C3 antibody for PD-L1 and scored by a board-certified pathologist. Based on the percentage and intensity of membrane staining, H-score, ranging from 0 to 300, was assigned to tumor samples ;i | Full Paper |
2019 | Lv, JW;Li, JY;Luo, LN;Wang, ZX;Chen, YP; | Comparative safety and efficacy of anti-PD-1 monotherapy, chemotherapy alone, and their combination therapy in advanced nasopharyngeal carcinoma: findings from recent advances in landmark trials | J Immunother Cancer | 31238988 | D-L1 expression was assessed baseline on an archived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy sample using a laboratory-developed prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA). PD-L1 | Full Paper |
2019 | Clouthier, DL;Lien, SC;Yang, SYC;Nguyen, LT;Manem, VSK;Gray, D;Ryczko, M;Razak, ARA;Lewin, J;Lheureux, S;Colombo, I;Bedard, PL;Cescon, D;Spreafico, A;Butler, MO;Hansen, AR;Jang, RW;Ghai, S;Weinreb, I;Sotov, V;Gadalla, R;Noamani, B;Guo, M;Elston, S;Giesler, A;Hakgor, S;Jiang, H;McGaha, T;Brooks, DG;Haibe-Kains, B;Pugh, TJ;Ohashi, PS;Siu, LL; | An interim report on the investigator-initiated phase 2 study of pembrolizumab immunological response evaluation (INSPIRE) | J Immunother Cancer | 30867072 | IHC. For screening biopsies only, the FFPE blocks were used for PD-L1 IHC (clone 22C3) on 4-5 μm sections mounted on positively charged ProbeOn slides (QualTek, Goleta, CA). QualTek provided a modified proportion score ;from the same site as the | Full Paper |
2019 | Rowley, MJ;Lyu, X;Rana, V;Ando-Kuri, M;Karns, R;Bosco, G;Corces, VG; | Condensin II Counteracts Cohesin and RNA Polymerase II in the Establishment of 3D Chromatin Organization | Cell Rep | 30865881 | We would like to thank the HudsonAlpha Institute for Biotechnology Genomic Services Lab for their help with Illumina sequencing. This work was supported by NIH Pathway to Independence Award K99/R00 GM127671 (M.J.R.) and U.S. Public Health Service Awa | Full Paper |
2019 | Wu, T;Dejanovic, B;Gandham, VD;Gogineni, A;Edmonds, R;Schauer, S;Srinivasan, K;Huntley, MA;Wang, Y;Wang, TM;Hedehus, M;Barck, KH;Stark, M;Ngu, H;Foreman, O;Meilandt, WJ;Elstrott, J;Chang, MC;Hansen, DV;Carano, RAD;Sheng, M;Hanson, JE; | Complement C3 Is Activated in Human AD Brain and Is Required for Neurodegeneration in Mouse Models of Amyloidosis and Tauopathy | Cell Rep | 31433986 | Human CSF cohort 1 Folio Biosciences n/a Human CSF cohort 2 PrecisionMed n/a Frozen human brain tissue Folio Biosciences n/ | Full Paper |
2019 | Angelova, E;Audette, C;Kovtun, Y;Daver, N;Wang, SA;Pierce, S;Konoplev, SN;Khogeer, H;Jorgensen, JL;Konopleva, M;Zweidler-McKay, PA;Medeiros, LJ;Kantarjian, HM;Jabbour, EJ;Khoury, JD; | CD123 expression patterns and selective targeting with a CD123-targeted antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemia | Haematologica | 30361418 | ... In vitro evaluation of primary B-cell acute lymphoblastic leukemia samples Bone marrow mononuclear cells from 11 newly diagnosed and 10 relapsed/refractory B-ALL patients were obtained from MDACC or ConversantBio. The number of CD123 antibody-bi | Full Paper |
2019 | Bao, L;Tian, C;Liu, S;Zhang, Y;Elaswad, A;Yuan, Z;Khalil, K;Sun, F;Yang, Y;Zhou, T;Li, N;Tan, S;Zeng, Q;Liu, Y;Li, Y;Li, Y;Gao, D;Dunham, R;Davis, K;Waldbieser, G;Liu, Z; | The Y chromosome sequence of the channel catfish suggests novel sex determination mechanisms in teleost fish | BMC Biol. | 30683095 | ;... Bioanalyzer. Equal amounts of RNA from each 5-day period were pooled from 10 to 14 dpf and 15 to 19 dpf for male and female, respectively. These four pooled samples were outsourced for RNA-Seq at the HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2019 | Rieker, C;Migliavacca, E;Vaucher, A;Mayer, FC;Baud, G;Marquis, J;Charpagne, A;Hegde, N;Guignard, L;McLachlan, M;Pooler, AM; | Apolipoprotein E4 Expression Causes Gain of Toxic Function in Isogenic Human Induced Pluripotent Stem Cell-Derived Endothelial Cells | Arterioscler. Thromb. Vasc. Biol. | 31315437 | Immunohistochemistry Analysis of Postmortem Human Brain Tissue Human hippocampal brain tissue from AD (Braak stage V-VI; both male and female) individuals was purchased from Folio Biosciences, genotyped for ApoE, and processed for cryo- sectioning | Full Paper |
2019 | Smith, A;Schiller, G;Vercellotti, G;Kwiatkowski, J;Krishnamurti, L;Esrick, E;Williams, D;Miller, W;Woolfson, A;Walters, M; | Preliminary Results of a Phase 1/2 Clinical Study of Zinc Finger Nuclease-Mediated Editing of BCL11A in Autologous Hematopoietic Stem Cells for Transfusion-Dependent Beta Thalassemia | Blood | Introduction: Persistently high fetal hemoglobin (HbF) expression can ameliorate severe transfusion-dependent beta thalassemia (TDT). BCL11A, a master regulator of the fetal-to-adult hemoglobin switch, is a rational gene-editing target in beta globin | Full Paper | |
2019 | Kang, J;Yeom, G;Jang, H;Oh, J;Park, CJ;Kim, MG; | Development of Replication Protein A-Conjugated Gold Nanoparticles for Highly Sensitive Detection of Disease Biomarkers | Anal. Chem. | 31269392 | Patient samples for NP and cTnI were obtained from Discovery Life Sciences, Inc. The study protocol was thoroughly explained to the patients, and signed written informed consent forms were obtained in accordance with the approved guidelines and relev | Full Paper |
2019 | Planes-Laine, G;Rochigneux, P;Bertucci, F;Chrétien, AS;Viens, P;Sabatier, R;Gonçalves, A; | PD-1/PD-L1 Targeting in Breast Cancer: The First Clinical Evidences Are Emerging. A Literature Review | Cancers (Basel) | 31336685 | ts included locally advanced or metastatic disease, after a failure of or inability to receive standard therapy, ECOG PS 0 or 1, ≥ 1 measurable lesion and PD-L1 positivity, using a prototype assay (QualTek Molecular Laboratories, Goleta, CA, USA) and | Full Paper |
2019 | Bernard-Gauthier, V;Mossine, AV;Knight, A;Patnaik, D;Zhao, WN;Cheng, C;Krishnan, HS;Xuan, LL;Chindavong, PS;Reis, SA;Chen, JM;Shao, X;Stauff, J;Arteaga, J;Sherman, P;Salem, N;Bonsall, D;Amaral, B;Varlow, C;Wells, L;Martarello, L;Patel, S;Liang, SH;Kurumbail, RG;Haggarty, SJ;Scott, PJH;Vasdev, N; | Structural Basis for Achieving GSK-3β Inhibition with High Potency, Selectivity, and Brain Exposure for Positron Emission Tomography Imaging and Drug Discovery | J. Med. Chem. | 31535859 | For autoradiography experiments, fresh-frozen postmortem human brain tissue blocks were acquired from Folio-Conversant and the University of California at San Francisco NDBB. In vitro autoradiography studies were performed as described in the Support | Full Paper |
2019 | McCoy, CR;Glover, ME;Flynn, LT;Simmons, RK;Cohen, JL;Ptacek, T;Lefkowitz, EJ;Jackson, NL;Akil, H;Wu, X;Clinton, SM; | Altered DNA Methylation in the Developing Brains of Rats Genetically Prone to High versus Low Anxiety | J. Neurosci. | 30683683 | Starting material for the enrichment protocol was 1g of sonicated DNA, and captured fragments were eluted using 2500 mM NaCl per the manufacturer’s instructions and as described previously (Brinkman et al., 2010). Captured material was purified using | Full Paper |
2019 | Chiu, H;Trisal, P;Bjorklund, C;Carrancio, S;Toraño, EG;Guarinos, C;Papazoglou, D;Hagner, PR;Beldi-Ferchiou, A;Tarte, K;Delfau-Larue, MH;Morschhauser, F;Ramsay, AG;Gandhi, AK; | Combination lenalidomide-rituximab immunotherapy activates anti-tumour immunity and induces tumour cell death by complementary mechanisms of action in follicular lymphoma | Br. J. Haematol. | 30767211 | Viably‐frozen PBMC from naive and relapsed/refractory FL and MZL patients were purchased from ConversantBio (Huntsville, AL, USA), ProteoGenex, (Inglewood, CA, USA) and BioreclammationIVT (Hicksville, NY, USA). Cell lines and cell culture | Full Paper |
2019 | Liang, X;Zhuang, J;Löffler, FE;Zhang, Y;DeBruyn, JM;Wilhelm, SW;Schaeffer, SM;Radosevich, M; | Viral and bacterial community responses to stimulated Fe(III)-bioreduction during simulated subsurface bioremediation | Environ. Microbiol. | 30773777 | Whole soil DNA was obtained by extracting both soil aggregates and associated pore water (Cápiro et al., 2014) using PowerLyser PowerSoil DNA isolation kit (MoBio Laboratories Inv. Carlsbad, CA) following the manufacturer’s instructions. The extracte | Full Paper |
2019 | Nowinski, B;Motard-Côté, J;Landa, M;Preston, CM;Scholin, CA;Birch, JM;Kiene, RP;Moran, MA; | Microdiversity and temporal dynamics of marine bacterial dimethylsulfoniopropionate genes | Environ. Microbiol. | 30761723 | This article is protected by copyright. All rights reserved. Page 23. HiSeq Rapid Run at HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA). Metagenomic data are deposited in the NCBI SRA under project number PRJNA505827. Single Amplified | Full Paper |
2019 | Xu, H;Muise, ES;Javaid, S;Chen, L;Cristescu, R;Mansueto, MS;Follmer, N;Cho, J;Kerr, K;Altura, R;Machacek, M;Nicholson, B;Addona, G;Kariv, I;Chen, H; | Identification of predictive genetic signatures of Cytarabine responsiveness using a 3D acute myeloid leukaemia model | J. Cell. Mol. Med. | 31449347 | 2 METHODS. 2.1 Cells and reagents. Normal and AML patient BMMCs were purchased from Folio Conversant and ProteoGenex. Recombinant human fibronectin was purchased from Millipore. Rat tail collagen type 1 and matrigel were purchased from Corning Inc | Full Paper |
2019 | Kim, YJ;Keam, B;Ock, CY;Song, S;Kim, M;Kim, SH;Kim, KH;Kim, JS;Kim, TM;Kim, DW;Lee, JS;Heo, DS; | A phase II study of pembrolizumab and paclitaxel in patients with relapsed or refractory small-cell lung cancer | Lung Cancer | 31494530 | Informed consent for specimens be obtained during screening for protocol enrollment from all subjects or legally acceptable representative, at a trial visit by the investigator or designate. Pretreatment blood sampling and tumor biopsies will be done | Full Paper |
2019 | Yang, X;Saito, Y;Rao, A;Kim, HJ;Singh, P;Scott, E;Larson, M;Pan, W;Desai, M;Hubbell, E; | Alignment-free filtering for cfNA fusion fragments | Bioinformatics | 31510681 | We have obtained 40 ml of whole blood collected in Streck tubes, separated into plasma and extracted cfRNA samples from two Stage IV Prostate cancer patients and three healthy controls from Conversant Bio and generated cfRNA sequencing data with UMIs | Full Paper |
2019 | Qi, Z;Wang, L;Desai, K;Cogswell, J;Stern, M;Lawson, B;Kerkar, SP;Vitazka, P; | Reliable Gene Expression Profiling from Small and Hematoxylin and Eosin-Stained Clinical Formalin-Fixed, Paraffin-Embedded Specimens Using the HTG EdgeSeq Platform | J Mol Diagn | 31255795 | Rockville, MD). Small-cell lung cancer (SCLC) samples (n = 4) were purchased from Conversant Biologics, Inc. (Huntsville, AL) or Proteogenex (Inglewood, CA). FFPE muscle tissue was obtained from Proteogenex. RNA samples | Full Paper |
2019 | Dietz, L;Dömel, JS;Leese, F;Mahon, AR;Mayer, C; | Phylogenomics of the longitarsal Colossendeidae: The evolutionary history of an Antarctic sea spider radiation | Mol. Phylogenet. Evol. | 31002869 | Sea spiders (Pycnogonida) constitute a group of marine benthic arthropods that has a particularly high species diversity in the Southern Ocean. The "longitarsal" group of the sea spider family Colossendeidae is especially abundant in this region. How | Full Paper |
2019 | Varga, A;Piha-Paul, S;Ott, PA;Mehnert, JM;Berton-Rigaud, D;Morosky, A;Yang, P;Ruman, J;Matei, D; | Pembrolizumab in patients with programmed death ligand 1-positive advanced ovarian cancer: Analysis of KEYNOTE-028 | Gynecol. Oncol. | 30522700 | disease based on Response Evaluation Criteria in Solid Tumors version 1.1 (RECIST v1.1), PD-L1 positivity, determined immunohistochemically and defined as membranous staining on ≥1% modified proportion score or interface pattern (QualTek Molecular La | Full Paper |
2019 | Lee, MJ;Miller, Z;Park, JE;Bhattarai, D;Lee, W;Kim, KB; | H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growth | Sci Rep | 30858500 | ... on-linear regression based on n = 3 replicates per compound per concentration. (d) Heat map showing proteasome catalytic subunit activity profiles of 6 PI-naïve patient MM samples purchased from Conversant Biologics and AllCells (left). The numbe | Full Paper |
2019 | Liang, X;Radosevich, M;Löffler, F;Schaeffer, SM;Zhuang, J; | Impact of microbial iron oxide reduction on the transport of diffusible tracers and non-diffusible nanoparticles in soils | Chemosphere | 30597359 | Inc. Carlsbad, California, USA). The DNA samples were quantified using PicoGreen Assay Kit (Carlsbad, California, USA) and sent out for sequencing at HudsonAlpha Genomic Services Lab (Huntsville, Alabama, USA). The | Full Paper |
2019 | Nicolini, F;Bocchini, M;Bronte, G;Delmonte, A;Guidoboni, M;Crinò, L;Mazza, M; | Malignant Pleural Mesothelioma: State-of-the-Art on Current Therapies and Promises for the Future | Front Oncol | 32039010 | With a centrally-assessed 22C3 antibody-based immunohistochemistry assay (Qualtek laboratory), PDL1 expression was available in 62 patients: 45% were found negative ( | Full Paper |
2019 | Gani, AW;Wei, W;Shi, RZ;Ng, E;Nguyen, M;Chua, MS;So, S;Wang, SX; | An Automated, Quantitative, and Multiplexed Assay Suitable for Point-of-Care Hepatitis B Virus Diagnostics | Sci Rep | 31666635 | HBV DNA levels). As controls, we recruited 5 healthy volunteers who had no prior history of HBV and who were vaccinated against HBV. Serum samples from healthy Asian donors were also purchased from Conversant Biologics (Huntsville, AL). Supplementa | Full Paper |
2019 | Li, Y;Kocot, KM;Tassia, MG;Cannon, JT;Bernt, M;Halanych, KM; | Mitogenomics Reveals a Novel Genetic Code in Hemichordata | Genome Biol Evol | 30476024 | Variant codes in mitochondrial genomes within deuterostomes and photos from representatives of hemichordates. (A) Phylogenetic relationships according to the current understanding of deuterostome phylogeny based on multiple studies, red bars show cod | Full Paper |
2019 | Juttukonda, LJ;Green, ER;Lonergan, ZR;Heffern, MC;Chang, CJ;Skaar, EP; | Acinetobacter baumannii OxyR Regulates the Transcriptional Response to Hydrogen Peroxide | Infect. Immun. | 30297527 | RNA-seq library construction and sequencing were performed by HudsonAlpha (Huntsville, AL). The concentration and integrity of the total RNA were estimated with a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA) and an Agilent 2100 bioanalyzer (Appli | Full Paper |
2019 | Patel, S;Knight, A;Krause, S;Teceno, T;Tresse, C;Li, S;Cai, Z;Gouasmat, A;Carroll, VM;Barret, O;Gottmukkala, V;Zhang, W;Xiang, X;Morley, T;Huang, Y;Passchier, J; | Preclinical In Vitro and In Vivo Characterization of Synaptic Vesicle 2A-Targeting Compounds Amenable to F-18 Labeling as Potential PET Radioligands for Imaging of Synapse Integrity | Mol Imaging Biol | 31728839 | The same procedure was used for human post-mortem tissue (Folio Biosciences, Miami Brain Bank, and UCSF Memory and Aging Center, USA). In Vitro Homogenate Binding. All homogenate studies were carried out in 96-well format as previously described [14] | Full Paper |
2019 | Gao, L;Yuan, Z;Zhou, T;Yang, Y;Gao, D;Dunham, R;Liu, Z; | FOXO genes in channel catfish and their response after bacterial infection | Dev. Comp. Immunol. | 30905685 | ; Plus Kit (Qiagen). RNA-seq library was prepared and sequenced by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) using an Illumina HiSeq 2000 instrument with 100 bp paired end (PE) reads. The trimmed high | Full Paper |
2019 | Havird, JC;Meyer, E;Fujita, Y;Vaught, RC;Henry, RP;Santos, SR; | Disparate responses to salinity across species and organizational levels in anchialine shrimps | J. Exp. Biol. | 31727759 | Assembly of reference transcriptomes was previously described for H. rubra (Havird and Santos, 2016a) and the four species from Japan (Havird et al., 2014b). Briefly, total RNA was extracted from whole animals 1 month after preservation in RNAlater v | Full Paper |
2019 | Rey, EG;Finkelstein, JL;Erickson, D; | Fluorescence lateral flow competitive protein binding assay for the assessment of serum folate concentrations | PLoS ONE | 31166978 | Serum sample preparation. Calibration curves made with human serum spiked with FA and 5-MTHF were performed as part of a previous work[26]. Human serum samples of varying folate concentration were purchased from Discovery Life Sciences Inc. (Los Osos | Full Paper |
2019 | Mehnert, JM;Varga, A;Brose, MS;Aggarwal, RR;Lin, CC;Prawira, A;de Braud, F;Tamura, K;Doi, T;Piha-Paul, SA;Gilbert, J;Saraf, S;Thanigaimani, P;Cheng, JD;Keam, B; | Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with advanced, PD-L1-positive papillary or follicular thyroid cancer | BMC Cancer | 30832606 | biopsy sample. PD-L1 expression was assessed using a prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, California) [22] and the 22C3 antibody (Merck & Co., Inc., Kenilworth, New Jersey). PD ;screening period using ei | Full Paper |
2019 | Huang, RSP;Holmes, BF;Powell, C;Marati, RV;Tyree, D;Admire, B;Streator, A;Newell, AEH;Perez, J;Dalvi, D;ElGabry, EA; | Delta-like Protein 3 Prevalence in Small Cell Lung Cancer and DLL3 (SP347) Assay Characteristics | Arch. Pathol. Lab. Med. | 30958693 | formalin), paraffin-embedded SCLC whole-section samples was procured from an internal tissue bank at Ventana Medical Systems, Inc, and the following commercial tissue banks: Asterand Bioscience; Avaden BioSciences; Boca Biolistics; Conversant Bio; GL | Full Paper |
2019 | Tsypin, M;Asmellash, S;Meyer, K;Touchet, B;Roder, H; | Extending the information content of the MALDI analysis of biological fluids via multi-million shot analysis | PLoS ONE | 31815946 | 100 serum samples were purchased from the commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrics (Fort Worth, TX). Samples were collected under ethics-approved protocols according to the requirements of Conversant Bio and Oncology M | Full Paper |
2019 | Li, S;Song, C;Yang, S;Yu, W;Zhang, W;Zhang, G;Xi, Z;Lu, E; | Supercritical CO2 foamed composite scaffolds incorporating bioactive lipids promote vascularized bone regeneration via Hif-1α upregulation and enhanced type H vessel formation | Acta biomaterialia | 31154054 | Upon reaching 80-90% confluency, the rBMSCs were passaged with passages 2-4 being used for further experiments. HUVECs (AllCells, Shanghai, China) were cultured in endothelial cell medium (ECM, ScienCell, USA) under the same conditions and used for i | Full Paper |
2019 | Desai, SP;Coston, A;Berlin, A; | Micro-Electrical Impedance Spectroscopy and Identification of Patient-Derived, Dissociated Tumor Cells | IEEE Trans Nanobioscience | 31180894 | Stored waveforms are further analyzed using statistical data analysis techniques. C. Dissociated Tumor Cells and Cell Culture Dissociated tumor cells (DTCs) were acquired directly from Conversant Bio (Huntsville, AL) for a range of indications and ca | Full Paper |
2019 | Lee, JS;Rosoff, D;Luo, A;Longley, M;Phillips, M;Charlet, K;Muench, C;Jung, J;Lohoff, FW; | PCSK9 is Increased in Cerebrospinal Fluid of Individuals With Alcohol Use Disorder | Alcohol. Clin. Exp. Res. | 30933362 | the NIH Institutional Review Board (IRB). All participants provided written informed consent before participation. CSF of deidentified controls was purchased from Discovery Life Sciences, Inc. (Research Biospecimen Sample Bank, Los Osos, CA, USA) | Full Paper |
2019 | Burbelo, PD;Chaturvedi, A;Notkins, AL;Gunti, S; | Luciferase-Based Detection of Antibodies for the Diagnosis of HPV-Associated Head and Neck Squamous Cell Carcinoma | Diagnostics (Basel) | 31390810 | The serum samples from colon cancer (CC), systemic lupus erythematosus (SLE), and HNSCC patients used in this report were obtained from Conversant Bio (601 Genome Way Suite 1200, Huntsville, Alabama 35806). Subjects | Full Paper |
2019 | Kao, JH;Lin, CY;Chuang, WL;Cheng, YY;Hu, JY;Liang, WK;Friebe, P;Palmer, S;Huang, CS; | Clinical evaluation of IntelliPlexTM HCV genotyping kit for hepatitis C virus genotyping | Diagn. Microbiol. Infect. Dis. | 30975461 | as certified by the commercial supplier in the accompanying Certificate of Analysis (CoA)), including genotypes 3, 4 and 5 were spiked into the sample pool (samples were obtained from SeraCare Life Sciences Inc., [Cambridge, MA United States]; Discov | Full Paper |
2019 | Lagatie, O;Verheyen, A;Nijs, E;Batsa Debrah, L;Debrah, YA;Stuyver, LJ; | Performance evaluation of 3 serodiagnostic peptide epitopes and the derived multi-epitope peptide OvNMP-48 for detection of Onchocerca volvulus infection | Parasitol. Res. | 31089811 | and informed consent, which was signed by all subjects. For samples obtained from Tissue Solutions Ltd. (Glasgow, Scotland), Discovery Life Sciences, Inc. (Los Osos, USA), Bioreclamation IVT (Baltimore, USA), Mayo Clinic | Full Paper |
2019 | Montazeri, M;Marbut, C;Huitink, D; | Interconnect Fatigue Failure Parameter Isolation for Power Device Reliability Prediction in Alternative Accelerated Mechanical Cycling Test | Journal of Electronic Packaging | IEEE Forth Workshop on Wide Bandgap Power Devices and Applications ( WiPDA), Fayetteville, AR, Nov. 7-9, pp. 194– 199. 19. Qualitek, 2014, “Technical Data Sheet: Sn42/Bi58 Solder Wire,” Qualtek Electronics Corporation, Addison, IL, Accessed Ma | Full Paper | |
2019 | Mohan, S;Lawton, R;Palmer, C;Rojas, AC; | Competitive ELISA method for novel estrogen-negative breast cancer biomarker quantitation | J. Immunol. Methods | 31533022 | system) (Rakha et al., 2008), and categorized based on estrogen-hormone-receptor orientation (viz., ER − versus ER + , n = 50), were obtained from cooperative human tissue network (Philadelphia, PA), Tissue-for-Research (Atlanta, GA), and Conversant | Full Paper |
2019 | Nicol, AF;de Andrade, CV;Gomes, SC;Brusadelli, MG;Lodin, HM;Wells, SI;Nuovo, GJ; | The distribution of novel biomarkers in carcinoma-in-situ, microinvasive, and squamous cell carcinoma of the uterine cervix | Ann Diagn Pathol | 30579259 | National Institute of Infectious Diseases Evandro Chagas - INI-Fiocruz, Rio de Janeiro, Brazil.++National Institute of Health of Women, Children, and Adolescents, Fernandes Figueira - IFF-FIOCRUZ, Rio de Janeiro, Brazil.++National Institute of Health | Full Paper |
2019 | Mimitou, EP;Cheng, A;Montalbano, A;Hao, S;Stoeckius, M;Legut, M;Roush, T;Herrera, A;Papalexi, E;Ouyang, Z;Satija, R;Sanjana, NE;Koralov, SB;Smibert, P; | Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cells | Nature methods | 31011186 | FBS 10% DMSO and aliquots of 1-1.5 million cells were thawed on the day of the experiment. PBMCs were obtained cryopreserved from AllCells (USA) and used immediately after thawing. NIH-3T3 and HEK293FT cells expressing non-targeting sgRNAs were maint | Full Paper |
2019 | Symeonides, S; | Choice of Law in the American Courts in 2018: Thirty-Second Annual Survey | The American Journal of Comparative Law | In re EP Floors Corp., No. 14-18-00610-CV, 2018 WL 4354688 (Tex. App. Sept. 13, 2018); In re Rosewood Private Invs., Inc., No. 05-18-00166-CV, 2018 WL 4403749 (Tex. App. Sept. 17, 2018); Bundy v. Adesa Houston D/B/A Adesa, Inc., No. 01-17-00863-CV, 2 | Full Paper | |
2019 | Yant, SR;Mulato, A;Hansen, D;Tse, WC;Niedziela-Majka, A;Zhang, JR;Stepan, GJ;Jin, D;Wong, MH;Perreira, JM;Singer, E;Papalia, GA;Hu, EY;Zheng, J;Lu, B;Schroeder, SD;Chou, K;Ahmadyar, S;Liclican, A;Yu, H;Novikov, N;Paoli, E;Gonik, D;Ram, RR;Hung, M;McDougall, WM;Brass, AL;Sundquist, WI;Cihlar, T;Link, JO; | A highly potent long-acting small-molecule HIV-1 capsid inhibitor with efficacy in a humanized mouse model | Nature medicine | 31501601 | Human PBMCs were collected from consenting healthy volunteers under informed consent; their use was approved by an institutional review board at AllCells. Human PBMCs, CD4 + T Save | Full Paper |
2019 | Qu, L;Yi, Z;Zhu, S;Wang, C;Cao, Z;Zhou, Z;Yuan, P;Yu, Y;Tian, F;Liu, Z;Bao, Y;Zhao, Y;Wei, W; | Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs | Nature biotechnology | 31308540 | 10% FBS and IL2 (1,000 U ml-1) and stimulated with CD3/CD28 DynaBeads (ThermoFisher, 11131D) for 2 d. Leukapheresis products from healthy donors were acquired from AllCells LLC China. All healthy donors provided informed consent | Full Paper |
2019 | Granja, JM;Klemm, S;McGinnis, LM;Kathiria, AS;Mezger, A;Corces, MR;Parks, B;Gars, E;Liedtke, M;Zheng, GXY;Chang, HY;Majeti, R;Greenleaf, WJ; | Single-cell multiomic analysis identifies regulatory programs in mixed-phenotype acute leukemia | Nature biotechnology | 31792411 | Description of healthy donors. PBMCs, BMMCs and CD34+ bone marrow cells were obtained from healthy donors with informed consent and compliance with relevant ethical regulations (AllCells). Individual information for each donor is provided in Suppleme | Full Paper |
2019 | Satpathy, AT;Granja, JM;Yost, KE;Qi, Y;Meschi, F;McDermott, GP;Olsen, BN;Mumbach, MR;Pierce, SE;Corces, MR;Shah, P;Bell, JC;Jhutty, D;Nemec, CM;Wang, J;Wang, L;Yin, Y;Giresi, PG;Chang, ALS;Zheng, GXY;Greenleaf, WJ;Chang, HY; | Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustion | Nature biotechnology | 31375813 | obtained from AllCells or the AllCells and isolated per manufacturer recommendations (as outlined in Supplementary Table 4). FACS-isolated populations were obtained from AllCells Save | Full Paper |
2019 | Miller, JC;Patil, DP;Xia, DF;Paine, CB;Fauser, F;Richards, HW;Shivak, DA;Bendaña, YR;Hinkley, SJ;Scarlott, NA;Lam, SC;Reik, A;Zhou, Y;Paschon, DE;Li, P;Wangzor, T;Lee, G;Zhang, L;Rebar, EJ; | Enhancing gene editing specificity by attenuating DNA cleavage kinetics | Nature biotechnology | 31359006 | CD34 + stem and progenitor cells were purified from mobilized peripheral blood leukapheresis products obtained from AllCells. Platelet depletion was performed, CD34 + cells were Save | Full Paper |
2019 | Kinney, MA;Vo, LT;Frame, JM;Barragan, J;Conway, AJ;Li, S;Wong, KK;Collins, JJ;Cahan, P;North, TE;Lauffenburger, DA;Daley, GQ; | A systems biology pipeline identifies regulatory networks for stem cell engineering | Nature biotechnology | 31267104 | Human CD34 + progenitors derived from mobilized peripheral blood (AllCells) were expanded for 4 d in StemSpan SFEM (StemCell Technologies) with the addition of interleukin (IL)-3 ( Save | Full Paper |
2019 | Lareau, CA;Duarte, FM;Chew, JG;Kartha, VK;Burkett, ZD;Kohlway, AS;Pokholok, D;Aryee, MJ;Steemers, FJ;Lebofsky, R;Buenrostro, JD; | Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibility | Nature biotechnology | 31235917 | CD34+ stem/progenitor cells, peripheral blood mononuclear cells (PBMC), and isolated peripheral blood CD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from Allcells ( Save;BMMCs, isolated bone marrow CD34 + stem/progenitor cells, PBMCs, and i | Full Paper |
2019 | Setty, M;Kiseliovas, V;Levine, J;Gayoso, A;Mazutis, L;Pe'er, D; | Characterization of cell fate probabilities in single-cell data with Palantir | Nature biotechnology | 30899105 | incorporate lineage information to model cell fate decisions. scRNA-seq of CD34+ human bone marrow cells Cryopreserved bone marrow stem/progenitor CD34+ cells from healthy donors were purchased from AllCells, LLC. (Cat. No. ABM022F) and stored in va | Full Paper |
2019 | Shah, NJ;Mao, AS;Shih, TY;Kerr, MD;Sharda, A;Raimondo, TM;Weaver, JC;Vrbanac, VD;Deruaz, M;Tager, AM;Mooney, DJ;Scadden, DT; | An injectable bone marrow-like scaffold enhances T cell immunity after hematopoietic stem cell transplantation | Nature biotechnology | 30742125 | t-specific antibodies. We isolated hematopoietic cells (Lin−Sca-1hic-kithi) using a FacsAria cell sorter (BD). Sorted cells were ≥ 95% pure. Human cord-blood derived CD34+ cells were purchased (Allcells) and expanded for seven days using expansion su | Full Paper |
2019 | Maun, HR;Jackman, JK;Choy, DF;Loyet, KM;Staton, TL;Jia, G;Dressen, A;Hackney, JA;Bremer, M;Walters, BT;Vij, R;Chen, X;Trivedi, NN;Morando, A;Lipari, MT;Franke, Y;Wu, X;Zhang, J;Liu, J;Wu, P;Chang, D;Orozco, LD;Christensen, E;Wong, M;Corpuz, R;Hang, JQ;Lutman, J;Sukumaran, S;Wu, Y;Ubhayakar, S;Liang, X;Schwartz, LB;Babina, M;Woodruff, PG;Fahy, JV;Ahuja, R;Caughey, GH;Kusi, A;Dennis, MS;Eigenbrot, C;Kirchhofer, D;Austin, CD;Wu, LC;Koerber, JT;Lee, WP;Yaspan, BL;Alatsis, KR;Arron, JR;Lazarus, RA;Yi, T; | An Allosteric Anti-tryptase Antibody for the Treatment of Mast Cell-Mediated Severe Asthma | Cell | 31585081 | Enriched CD34+ human cord blood stem cells were obtained from AllCells with more than 80% purity validated by flow cytometry analysis. CHO DKO cell line (Macaraeg et al., 2013) was a derivative of the CHO-K1 cell line (ATCC number CCL-61) was grown a | Full Paper |
2019 | Stuart, T;Butler, A;Hoffman, P;Hafemeister, C;Papalexi, E;Mauck, WM;Hao, Y;Stoeckius, M;Smibert, P;Satija, R; | Comprehensive Integration of Single-Cell Data | Cell | 31178118 | Hashtag 9 Cell Hashing antibody BioLegend Cat# 394617 Hashtag 10 Cell Hashing antibody BioLegend Cat# 394619 Biological Samples Human bone marrow cells AllCells Cat# ABM007F, lot 3008803 Critical Commercial Assays 3′ scRNA-seq kit 10x Genomics V2 che | Full Paper |
2019 | Abelin, JG;Harjanto, D;Malloy, M;Suri, P;Colson, T;Goulding, SP;Creech, AL;Serrano, LR;Nasir, G;Nasrullah, Y;McGann, CD;Velez, D;Ting, YS;Poran, A;Rothenberg, DA;Chhangawala, S;Rubinsteyn, A;Hammerbacher, J;Gaynor, RB;Fritsch, EF;Greshock, J;Oslund, RC;Barthelme, D;Addona, TA;Arieta, CM;Rooney, MS; | Defining HLA-II Ligand Processing and Binding Rules with Mass Spectrometry Enhances Cancer Epitope Prediction | Immunity | 31495665 | Human Healthy Donor Leukapheresis Material AllCells NA Human Healthy Donor Leukapheresis Material PPA Research NA Human Healthy Donor Leukapheresis Material StemExpress NA Human Healthy Donor Leukapheresis Material StemCell Technologies NA | Full Paper |
2019 | Lindner, JM;Cornacchione, V;Sathe, A;Be, C;Srinivas, H;Riquet, E;Leber, XC;Hein, A;Wrobel, MB;Scharenberg, M;Pietzonka, T;Wiesmann, C;Abend, J;Traggiai, E; | Human Memory B Cells Harbor Diverse Cross-Neutralizing Antibodies against BK and JC Polyomaviruses | Immunity | 30824324 | Purified peripheral blood mononuclear cells from leukapharesis AllCells, Inc. N/A leukapharesis and matched serum samples were obtained from AllCells LLC (Alameda, CA, USA). Save | Full Paper |
2019 | Li, R;Wang, Y;Zhang, X;Feng, M;Ma, J;Li, J;Yang, X;Fang, F;Xia, Q;Zhang, Z;Shang, M;Jiang, S; | Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasis | Molecular cancer | 30704479 | HUVECs were purchased from ATCC and cultivated in endothelial cell complete medium containing endothelial cell growth supplement (Allcells, USA). For hypoxic culture, HCC cells Save | Full Paper |
2019 | Jiang, Q;Isquith, J;Zipeto, MA;Diep, RH;Pham, J;Delos Santos, N;Reynoso, E;Chau, J;Leu, H;Lazzari, E;Melese, E;Ma, W;Fang, R;Minden, M;Morris, S;Ren, B;Pineda, G;Holm, F;Jamieson, C; | Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor Propagation | Cancer cell | 30612940 | Institutional Review Board approved protocol (#131550) that meets the requirements as stated in 45 CFR 46.404 and 21 CFR 50.51. De-identified (IRB exempt) human cord blood and normal aged-match samples were purchased as purified CD34+ cells from AllC | Full Paper |
2019 | Adelman, ER;Huang, HT;Roisman, A;Olsson, A;Colaprico, A;Qin, T;Lindsley, RC;Bejar, R;Salomonis, N;Grimes, HL;Figueroa, ME; | Aging Human Hematopoietic Stem Cells Manifest Profound Epigenetic Reprogramming of Enhancers That May Predispose to Leukemia | Cancer discovery | 31085557 | AML, which is more frequent in the elderly, is characterized by epigenetic deregulation. We demonstrate that epigenetic reprogramming of human HSCs occurs with age, affecting Save | Full Paper |
2019 | Gong, X;Du, J;Parsons, SH;Merzoug, FF;Webster, Y;Iversen, PW;Chio, LC;Van Horn, RD;Lin, X;Blosser, W;Han, B;Jin, S;Yao, S;Bian, H;Ficklin, C;Fan, L;Kapoor, A;Antonysamy, S;Mc Nulty, AM;Froning, K;Manglicmot, D;Pustilnik, A;Weichert, K;Wasserman, SR;Dowless, M;Marugán, C;Baquero, C;Lallena, MJ;Eastman, SW;Hui, YH;Dieter, MZ;Doman, T;Chu, S;Qian, HR;Ye, XS;Barda, DA;Plowman, GD;Reinhard, C;Campbell, RM;Henry, JR;Buchanan, SG; | Aurora A Kinase Inhibition Is Synthetic Lethal with Loss of the RB1 Tumor Suppressor Gene | Cancer discovery | 30373917 | The identification of a synthetic lethal interaction between RB1 and AURKA inhibition, and the discovery of a drug that can be dosed continuously to achieve uninterrupted inhibition of Save | Full Paper |
2019 | Mascarenhas, J;Lu, M;Kosiorek, H;Virtgaym, E;Xia, L;Sandy, L;Mesa, R;Petersen, B;Farnoud, N;Najfeld, V;Rampal, R;Dueck, A;Hoffman, R; | Oral idasanutlin in patients with polycythemia vera | Blood | 31167802 | Baseline MDM2 protein levels in the enrolled PV/ET patients and 7 normal samples (peripheral blood, n = 4; bone marrow, n = 3) purchased from ALLcells (Alameda, CA) were evaluated using monoclonal antibody staining and flow cytometric analysis.24 Bri | Full Paper |
2019 | Drissen, R;Thongjuea, S;Theilgaard-Mönch, K;Nerlov, C; | Identification of two distinct pathways of human myelopoiesis | Science immunology | 31126997 | Bone marrow samples were from AllCells or taken from healthy male volunteers at the age of 21 to 29 years old, who provided written informed consent in accordance with local Save | Full Paper |
2019 | Hu, CW;Qiu, Y;Ligeralde, A;Raybon, AY;Yoo, SY;Coombes, KR;Qutub, AA;Kornblau, SM; | A quantitative analysis of heterogeneities and hallmarks in acute myelogenous leukaemia | Nature biomedical engineering | 30988472 | was prepared as described in publications11,21. In addition to AML patient samples, each array includes the same ten cryopreserved normal bone marrow CD34+ samples (NLBM CD34+) from healthy subjects (AllCells, Alameda, CA). Mutation Analysis All mut | Full Paper |
2019 | Maekawa, T;Kato, S;Kawamura, T;Takada, K;Sone, T;Ogata, H;Saito, K;Izumi, T;Nagao, S;Takano, K;Okada, Y;Tachi, N;Teramoto, M;Horiuchi, T;Hikota-Saga, R;Endo-Umeda, K;Uno, S;Osawa, Y;Kobayashi, A;Kobayashi, S;Sato, K;Hashimoto, M;Suzu, S;Usuki, K;Morishita, S;Araki, M;Makishima, M;Komatsu, N;Kimura, F; | Increased SLAMF7high monocytes in myelofibrosis patients harboring JAK2V617F provide a therapeutic target of elotuzumab | Blood | 31270105 | The hNOG mouse was created by transplanting human cord blood (Allcells, Alameda, CA) into the NOG mouse established by backcrossing an interleukin-2R (IL-2R) γ-chain knockout Save | Full Paper |
2019 | Pang, WW;Czechowicz, A;Logan, AC;Bhardwaj, R;Poyser, J;Park, CY;Weissman, IL;Shizuru, JA; | Anti-CD117 antibody depletes normal and myelodysplastic syndrome human hematopoietic stem cells in xenografted mice | Blood | 30745302 | Normal human BM samples were purchased from AllCells (Alameda, CA). Human umbilical cord blood (UCB) samples and human BM samples from MDS patients were obtained Save | Full Paper |
2019 | Abdel-Azim, H;Dvorak, C;Bunin, N;Walters, M;Talano, J;Anderson, E;Malvar, J;Al Jassani, M;Chaudhury, S;Cairo, M;Kitko, C;Jacob, A;Monos, D;Wing, L;Pulsipher, M; | Next Generation Sequence Minimal Residual Disease (NGS-MRD) Predicts Outstanding Event Free Survival (EFS) Regardless of Hematopoietic Cell Transplantation (HCT) Preparative Approach or Graft Alpha/Beta Depletion in Children with Acute Lymphoblastic Leukemia (ALL) | Blood | MRD detected by flow cytometry (FC) or PCR has been associated with key outcomes after HCT for ALL. In a prospective multicenter trial (NCT02646839; Pediatric Blood and Marrow Transplant Consortium [PBMTC] ONC1401), we performed a planned sub-study o | Full Paper | |
2019 | Dunbar, A;Kosiorek, H;Krishnan, A;McGovern, E;Park, Y;Patel, M;Weinberg, R;Yacoub, A;Dueck, A;Mascarenhas, J;Levine, R;Hoffman, R;Rampal, R; | Identifying Cytokine Biomarkers of Response to Pegylated-Interferon Therapy in Polycythemia Vera and Essential Thrombocythemia: Correlative Analysis from the MPN-RC 111/112 Trials | Blood | Age-matched serum from healthy individuals (N=6) was obtained from AllCells TM for use as wild-type (WT) controls. Baseline cytokine samples were compared between groups of Save;Background: Pegylated Interferon-alpha (PEG-IFNa) improves hematologic | Full Paper | |
2019 | Park, SH;Lee, CM;Dever, DP;Davis, TH;Camarena, J;Srifa, W;Zhang, Y;Paikari, A;Chang, AK;Porteus, MH;Sheehan, VA;Bao, G; | Highly efficient editing of the β-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell disease | Nucleic acids research | 31147717 | K562 cells were cultured in RPMI1640 (ATCC, Cat No. 30-2001) supplemented with 10% FBS and L-glutamine. Frozen CD34+ cells from mobilized peripheral blood of normal individuals were purchased from AllCells. Peripheral blood CD34+ cells were acquired | Full Paper |
2019 | Shortridge, MD;Wille, PT;Jones, AN;Davidson, A;Bogdanovic, J;Arts, E;Karn, J;Robinson, JA;Varani, G; | An ultra-high affinity ligand of HIV-1 TAR reveals the RNA structure recognized by P-TEFb | Nucleic acids research | 30481318 | stimulate HIV-1 transcription. Twenty-four hours later, the percentage of cells expressing GFP was determined by flow cytometry as described above. Peptide uptake Frozen, de-identified human PBMCs (Allcells, Alameda, CA) were thawed and stimulated w | Full Paper |
2019 | McGrath, E;Shin, H;Zhang, L;Phue, JN;Wu, WW;Shen, RF;Jang, YY;Revollo, J;Ye, Z; | Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing | Nature communications | 31767844 | Briefly, CD34+ cells from a healthy male donor were purchased from AllCells (Alameda, CA, USA) and were cultured in StemSpan medium (STEMCELL Technologies, Vancouver, BC, Save | Full Paper |
2019 | Sachdeva, M;Busser, BW;Temburni, S;Jahangiri, B;Gautron, AS;Maréchal, A;Juillerat, A;Williams, A;Depil, S;Duchateau, P;Poirot, L;Valton, J; | Repurposing endogenous immune pathways to tailor and control chimeric antigen receptor T cell functionality | Nature communications | 31723132 | are able to integrate complex environmental inputs and to generate custom outputs in a highly regulated and specific manner. Methods Biological materials Cryopreserved human PBMCs were acquired from ALLCELLS (cat # PB006F) and used in accordance wit | Full Paper |
2019 | Gomez-Ospina, N;Scharenberg, SG;Mostrel, N;Bak, RO;Mantri, S;Quadros, RM;Gurumurthy, CB;Lee, C;Bao, G;Suarez, CJ;Khan, S;Sawamoto, K;Tomatsu, S;Raj, N;Attardi, LD;Aurelian, L;Porteus, MH; | Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type I | Nature communications | 31492863 | ck-treated samples, and 50% modification control were included. Data was analyzed using QuantaSoft (Bio-Rad). HSPC selection and culturing Human CD34+ HSPCs mobilized peripheral blood purchased from AllCells (Alameda, CA, USA) and thawed per manufac | Full Paper |
2019 | Zhang, Y;Roos, M;Himburg, H;Termini, CM;Quarmyne, M;Li, M;Zhao, L;Kan, J;Fang, T;Yan, X;Pohl, K;Diers, E;Jin Gim, H;Damoiseaux, R;Whitelegge, J;McBride, W;Jung, ME;Chute, JP; | PTPσ inhibitors promote hematopoietic stem cell regeneration | Nature communications | 31413255 | collected from the recipient mice and were analyzed for percentages of DsRed+ cells in the BM17. Human BM cultures and human BM transplantation assays Human BM mononuclear cells were purchased from AllCells. Cryopreserved human BM cells were recover | Full Paper |
2019 | Pellin, D;Loperfido, M;Baricordi, C;Wolock, SL;Montepeloso, A;Weinberg, OK;Biffi, A;Klein, AM;Biasco, L; | A comprehensive single cell transcriptional landscape of human hematopoietic progenitors | Nature communications | 31160568 | from MNCs with the human anti-CD34 MicroBeads Isolation Kit (Miltenyi Biotec) according to the manufacturer's specifications or were purchased from commercial sources (AllCells). Save | Full Paper |
2019 | Pavel-Dinu, M;Wiebking, V;Dejene, BT;Srifa, W;Mantri, S;Nicolas, CE;Lee, C;Bao, G;Kildebeck, EJ;Punjya, N;Sindhu, C;Inlay, MA;Saxena, N;DeRavin, SS;Malech, H;Roncarolo, MG;Weinberg, KI;Porteus, MH; | Gene correction for SCID-X1 in long-term hematopoietic stem cells | Nature communications | 30967552 | d to the purified AAV6 at a final concentration of 0.001%, aliquot and stored at −80 °C. CD34+ HSPCs Mobilized peripheral blood (mPB) and bone marow (BM) CD34+ HSPCs cells were purchased from AllCells (Alameda, CA, USA). Cells were thawed using pub | Full Paper |
2019 | Kimmey, SC;Borges, L;Baskar, R;Bendall, SC; | Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cells | Nature communications | 30862852 | Fresh bone marrow aspirates from two independent donors were obtained from AllCells (Alameda, CA) on the same day of collection, and immediately after receiving was placed in 37 Save | Full Paper |
2019 | Paschon, DE;Lussier, S;Wangzor, T;Xia, DF;Li, PW;Hinkley, SJ;Scarlott, NA;Lam, SC;Waite, AJ;Truong, LN;Gandhi, N;Kadam, BN;Patil, DP;Shivak, DA;Lee, GK;Holmes, MC;Zhang, L;Miller, JC;Rebar, EJ; | Diversifying the structure of zinc finger nucleases for high-precision genome editing | Nature communications | 30850604 | rer’s instructions. Target loci were then analyzed by PCR amplification followed by deep sequencing (MiSeq). Studies performed in T-cells Peripheral blood CD3 + Pan T-cells were purchased from AllCells (Alameda, CA). Transfections into activated T-c | Full Paper |
2019 | Czechowicz, A;Palchaudhuri, R;Scheck, A;Hu, Y;Hoggatt, J;Saez, B;Pang, WW;Mansour, MK;Tate, TA;Chan, YY;Walck, E;Wernig, G;Shizuru, JA;Winau, F;Scadden, DT;Rossi, DJ; | Selective hematopoietic stem cell ablation using CD117-antibody-drug-conjugates enables safe and effective transplantation with immunity preservation | Nature communications | 30728354 | als into 9 Gy, lethally irradiated, C57BL/6J (CD45.2+) wild-type recipients as previously described16. Human cord blood transplantation Single donor-derived cord blood CD34+ cells were obtained from AllCells (Alameda, CA) under local IRB-approved pr | Full Paper |
2019 | Zeng, Y;Yao, X;Liu, X;He, X;Li, L;Liu, X;Yan, Z;Wu, J;Fu, BM; | Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesis | Journal of extracellular vesicles | 31258881 | o inhibit tumor vasculogenesis may serve as a novel strategy to augment the long-term efficacy of anti-angiogenic and anti-autophagic therapies for tumors. Materials and methods Cell culture HUVECs (Allcells, Shanghai, China) were cultured in HUVEC | Full Paper |
2019 | Mu, Q;Cabana-Puig, X;Mao, J;Swartwout, B;Abdelhamid, L;Cecere, TE;Wang, H;Reilly, CM;Luo, XM; | Pregnancy and lactation interfere with the response of autoimmunity to modulation of gut microbiota | Microbiome | 31311609 | Human plasma samples were purchased from AllCells (Alameda, CA). For measuring anti-DNA IgG, anti-LPS IgG, and anti-flagellin IgG, high-binding plates were coated overnight at 4 Save | Full Paper |
2019 | Li, W;Wu, D;Zhu, S;Liu, Z;Luo, B;Lu, L;Zhou, C; | Sustained release of plasmid DNA from PLLA/POSS nanofibers for angiogenic therapy | Chemical Engineering Journal | Human umbilical vein endothelial cells (HUVECs, purchased from Allcells, shanghai, China) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (P/S, Gibco) at 37 °C in 5% CO2 | Full Paper | |
2019 | Wu, HW;Sheard, MA;Malvar, J;Fernandez, GE;DeClerck, YA;Blavier, L;Shimada, H;Theuer, CP;Sposto, R;Seeger, RC; | Anti-CD105 Antibody Eliminates Tumor Microenvironment Cells and Enhances Anti-GD2 Antibody Immunotherapy of Neuroblastoma with Activated Natural Killer Cells | Clinical cancer research : an official journal of the American Association for Cancer Research | 31068371 | Human umbilical vein endothelial cells (HUVEC) were purchased from AllCells, LLC and maintained in HUVEC Basal Medium containing 10% Stimulatory Supplement from AllCells in Save | Full Paper |
2019 | Zuch de Zafra, CL;Fajardo, F;Zhong, W;Bernett, MJ;Muchhal, US;Moore, GL;Stevens, J;Case, R;Pearson, JT;Liu, S;McElroy, PL;Canon, J;Desjarlais, JR;Coxon, A;Balazs, M;Nolan-Stevaux, O; | Targeting Multiple Myeloma with AMG 424, a Novel Anti-CD38/CD3 Bispecific T-cell-recruiting Antibody Optimized for Cytotoxicity and Cytokine Release | Clinical cancer research : an official journal of the American Association for Cancer Research | 30918018 | Frozen human T cells (AllCells) or frozen peripheral blood mononuclear cells (PBMC) from humans or cynomolgus (CepheusBio) were cocultured with target cells at an effector cell (E) Save | Full Paper |
2019 | Jin, Q;Martinez, CA;Arcipowski, KM;Zhu, Y;Gutierrez-Diaz, BT;Wang, KK;Johnson, MR;Volk, AG;Wang, F;Wu, J;Grove, C;Wang, H;Sokirniy, I;Thomas, PM;Goo, YA;Abshiru, NA;Hijiya, N;Peirs, S;Vandamme, N;Berx, G;Goosens, S;Marshall, SA;Rendleman, EJ;Takahashi, YH;Wang, L;Rawat, R;Bartom, ET;Collings, CK;Van Vlierberghe, P;Strikoudis, A;Kelly, S;Ueberheide, B;Mantis, C;Kandela, I;Bourquin, JP;Bornhauser, B;Serafin, V;Bresolin, S;Paganin, M;Accordi, B;Basso, G;Kelleher, NL;Weinstock, J;Kumar, S;Crispino, JD;Shilatifard, A;Ntziachristos, P; | USP7 Cooperates with NOTCH1 to Drive the Oncogenic Transcriptional Program in T-Cell Leukemia | Clinical cancer research : an official journal of the American Association for Cancer Research | 30224337 | were maintained in DMEM medium supplemented with 10% heat-inactivated FBS, 2% penicillin/streptomycin, and 1% GlutaMAX. Human pan T cells were purchased from AllCells.com (Alameda, CA). Primary human samples were collected by collaborating institutio | Full Paper |
2019 | Zheng, B;Yu, SF;Del Rosario, G;Leong, SR;Lee, GY;Vij, R;Chiu, C;Liang, WC;Wu, Y;Chalouni, C;Sadowsky, J;Clark, V;Hendricks, A;Poon, KA;Chu, W;Pillow, T;Schutten, MM;Flygare, J;Polson, AG; | An Anti-CLL-1 Antibody-Drug Conjugate for the Treatment of Acute Myeloid Leukemia | Clinical cancer research : an official journal of the American Association for Cancer Research | 29959143 | Bone marrow mononuclear cells (BM-MNC) from healthy human donors (AllCells) were prepared by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare). CD34 + Save | Full Paper |
2019 | Castillo, J;Wu, E;Lowe, C;Srinivasan, S;McCord, R;Wagle, MC;Jayakar, S;Edick, MG;Eastham-Anderson, J;Liu, B;Hutchinson, KE;Jones, W;Stokes, MP;Tarighat, SS;Holcomb, T;Glibicky, A;Romero, FA;Magnuson, S;Huang, SA;Plaks, V;Giltnane, JM;Lackner, MR;Mounir, Z; | CBP/p300 Drives the Differentiation of Regulatory T Cells through Transcriptional and Non-Transcriptional Mechanisms | Cancer research | 31182547 | Normal human peripheral blood CD4+/CD45RA+/CD25− naïve T cells were procured from Allcells (PB009-5). Cells were isolated using Immunomagnetic CD4+ Isolation Kit, CD45RO microbeads to deplete CD45RO+ cells, and cultured using Dynabeads human T activa | Full Paper |
2019 | Levi, J;Lam, T;Goth, SR;Yaghoubi, S;Bates, J;Ren, G;Jivan, S;Huynh, TL;Blecha, JE;Khattri, R;Schmidt, KF;Jennings, D;VanBrocklin, H; | Imaging of Activated T Cells as an Early Predictor of Immune Response to Anti-PD-1 Therapy | Cancer research | 31064845 | Neutrophils and eosinophils were isolated from human whole blood (AllCells, Alameda CA). Human neutrophil and eosinophil enrichment kit (StemCell Technologies) was used to purify the cells. Neutrophils were either treated with 1uM N-formyl- L-methion | Full Paper |
2019 | Boudreaux, SP;Duren, RP;Call, SG;Nguyen, L;Freire, PR;Narayanan, P;Redell, MS;Conneely, OM; | Drug targeting of NR4A nuclear receptors for treatment of acute myeloid leukemia | Leukemia | 29884904 | rmed consent at Texas Children’s Hospital in accordance with the Declaration of Helsinki and institutional review board regulations. Normal cord blood CD34+ stem/progenitor cells were obtained from AllCells. Connectivity Map (CMAP) Analysis The NR4A | Full Paper |
2019 | Shen, JC;Kamath-Loeb, AS;Kohrn, BF;Loeb, KR;Preston, BD;Loeb, LA; | A high-resolution landscape of mutations in the BCL6 super-enhancer in normal human B cells | Proceedings of the National Academy of Sciences of the United States of America | 31748270 | Human B Cells. The CD19+ B cells were purchased from AllCells and ReachBio. Peripheral blood mononuclear cells (PBMCs) were purified from whole blood drawn from 10 healthy donors (SI Appendix, Table S2). CD19+ B cells were purified by positive select | Full Paper |
2019 | Zhang, L;Dong, X;Lee, M;Maslov, AY;Wang, T;Vijg, J; | Single-cell whole-genome sequencing reveals the functional landscape of somatic mutations in B lymphocytes across the human lifespan | Proceedings of the National Academy of Sciences of the United States of America | 30992375 | Two frozen human umbilical cord blood mononuclear cell samples were obtained from AllCells and STEMCELL Technologies. Informed consent was obtained from all the donors. The Save | Full Paper |
2019 | Ye, S;Cohen, D;Belmar, NA;Choi, D;Tan, SS;Sho, M;Akamatsu, Y;Kim, H;Iyer, R;Cabel, J;Lake, M;Song, D;Harlan, J;Zhang, C;Fang, Y;Wahl, AF;Culp, P;Hollenbaugh, D;Chao, DT; | A Bispecific Molecule Targeting CD40 and Tumor Antigen Mesothelin Enhances Tumor-Specific Immunity | Cancer immunology research | 31462409 | than 10 passages from master cell banking. Fresh human peripheral blood mononuclear cells (PBMC) from healthy donors (about 109 per donor) were purchased from AllCells. Donor virus testing results of human immunodeficiency virus, hepatitis B virus, a | Full Paper |
2019 | Gui, X;Deng, M;Song, H;Chen, Y;Xie, J;Li, Z;He, L;Huang, F;Xu, Y;Anami, Y;Yu, H;Yu, C;Li, L;Yuan, Z;Xu, X;Wang, Q;Chai, Y;Huang, T;Shi, Y;Tsuchikama, K;Liao, XC;Xia, N;Gao, GF;Zhang, N;Zhang, CC;An, Z; | Disrupting LILRB4/APOE Interaction by an Efficacious Humanized Antibody Reverses T-cell Suppression and Blocks AML Development | Cancer immunology research | 31213474 | In the coculture assay, human T cells (5 × 10 4 cells per well) isolated from health donor peripheral blood (AllCells) were placed in the lower chambers of a 96-well transwell plate. Save | Full Paper |
2019 | Newman, M; | Abstract B178: Development and preclinical efficacy characterization of a systemically administered multiple Toll-like receptor (TLR) agonist for antitumor immunotherapy | Cancer Immunology Research | PBMCs from fresh normal peripheral blood leukapheresis paks (ALLCells, Alameda, CA) were isolated using a Ficoll gradient. Ten-fold increments of 1x10/mL to 1x10^8/mL untreated Save | Full Paper | |
2019 | Sun, Y;Xiao, Y;Sun, H;Zhao, Z;Zhu, J;Zhang, L;Dong, J;Han, T;Jing, Q;Zhou, J;Jing, Z; | miR-27a regulates vascular remodeling by targeting endothelial cells' apoptosis and interaction with vascular smooth muscle cells in aortic dissection | Theranostics | 31695809 | Prime HUVECs were purchased from ALLCELLS and cultured in endothelial cell media (ALLCELLS, Emeryville, California) at 37℃ in a humidified atmosphere with 5% CO 2 . HEK 293 Save | Full Paper |
2019 | Varricchio, L;Planutis, A;Manwani, D;Jaffray, J;Mitchell, WB;Migliaccio, AR;Bieker, JJ; | Genetic disarray follows mutant KLF1-E325K expression in a congenital dyserythropoietic anemia patient | Haematologica | 30872368 | no new patient material was obtained for the present study. Mononuclear cells from the peripheral blood (PBMC) had been isolated and cryopreserved as described.35 Non-patient PBMC were purchased from AllCells (PB003F). Human erythroid massive amplif | Full Paper |
2019 | Sapienza, MR;Abate, F;Melle, F;Orecchioni, S;Fuligni, F;Etebari, M;Tabanelli, V;Laginestra, MA;Pileri, A;Motta, G;Rossi, M;Agostinelli, C;Sabattini, E;Pimpinelli, N;Truni, M;Falini, B;Cerroni, L;Talarico, G;Piccioni, R;Amente, S;Indio, V;Tarantino, G;Brundu, F;Paulli, M;Berti, E;Facchetti, F;Dellino, GI;Bertolini, F;Tripodo, C;Rabadan, R;Pileri, SA; | Blastic plasmacytoid dendritic cell neoplasm: genomics mark epigenetic dysregulation as a primary therapeutic target | Haematologica | 30381297 | RNA of 4 normal plasmacytoid dendritic cell (pDCs) samples was purchased from AllCells (Alameda, CA, US) and used for comparison. For details, see Online Supplementary Table S6 Save;BPDCN cryopreserved cutaneous biopsies, sufficient only for RNA se | Full Paper |
2019 | Yao, X;Liu, R;Liang, X;Ding, J; | Critical Areas of Proliferation of Single Cells on Micropatterned Surfaces and Corresponding Cell Type Dependence | ACS applied materials & interfaces | 30964630 | Primary rat MSCs were purchased from Allcells Tech., Shanghai, People’s Republic of China; primary mouse embryo fibroblasts (EF) were purchased from Shanghai Gaining Biological Technology Co., Ltd.; human cervix epithelial carcinoma cells (Hela) and | Full Paper |
2019 | Deng, X;Chu, X;Wang, P;Ma, X;Wei, C;Sun, C;Yang, J;Li, Y; | MicroRNA-29a-3p Reduces TNFα-Induced Endothelial Dysfunction by Targeting Tumor Necrosis Factor Receptor 1 | Molecular therapy. Nucleic acids | 31760375 | ina), and HEK293 cells (ATCC, Manassas, VA, USA) were maintained in DMEM (HyClone, South Logan, UT, USA) containing 10% fetal bovine serum (FBS; PAA Laboratories, Austria). pHUVECs were obtained from AllCells (China) and cultured incomplete medium (A | Full Paper |
2019 | Mandal, S;Prathipati, PK;Belshan, M;Destache, CJ; | A potential long-acting bictegravir loaded nano-drug delivery system for HIV-1 infection: A proof-of-concept study | Antiviral research | 30991088 | TZM-bl cells were obtained from NIH AIDS reagent program (Kappes and Wu, 2018; Kappes et al., 2015). Peripheral blood mononuclear cells (PBMCs) were obtained from commercial vendor (AllCells Inc. Alameda, CA). TZM-bl cells were maintained in complete | Full Paper |
2019 | Bongen, E;Lucian, H;Khatri, A;Fragiadakis, GK;Bjornson, ZB;Nolan, GP;Utz, PJ;Khatri, P; | Sex Differences in the Blood Transcriptome Identify Robust Changes in Immune Cell Proportions with Aging and Influenza Infection | Cell reports | 31722210 | Sample preparation and CyTOF methods have been described previously (Fragiadakis et al., 2019). Briefly, peripheral blood was obtained from 86 human adult volunteers from the Stanford Blood Center, AllCells (exempt, non-human subjects research), and | Full Paper |
2019 | Shehata, L;Maurer, DP;Wec, AZ;Lilov, A;Champney, E;Sun, T;Archambault, K;Burnina, I;Lynaugh, H;Zhi, X;Xu, Y;Walker, LM; | Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability | Cell reports | 31553901 | Biological Samples Healthy donor bone marrow mononuclear cells AllCells https://www.allcells.com/ Healthy human peripheral blood mononuclear cells Dartmouth Hitchcock Medical Center N/A | Full Paper |
2019 | Shi, D;Dong, Y;Dai, P;Gao, J;Yin, J;Xie, M; | Dentin matrix protein 1 correlates with the severity of hemorrhagic fever with renal syndrome and promotes hyper-permeability of endothelial cells infected by Hantaan virus | Microbes and infection | 30735719 | Cells were cultured in AllCells complete endothelial cell medium and incubated at 37 C with 5% CO 2 . HUVECs (passage 3 or 4) of approximately 70-80% confluence were used for all Save | Full Paper |
2019 | Muñoz, DP;Yannone, SM;Daemen, A;Sun, Y;Vakar-Lopez, F;Kawahara, M;Freund, AM;Rodier, F;Wu, JD;Desprez, PY;Raulet, DH;Nelson, PS;van 't Veer, LJ;Campisi, J;Coppé, JP; | Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging | JCI insight | 31184599 | Freshly isolated human PBMCs were maintained in supplemented RPMI media and cultured in 3% O2 conditions. Monocytes were obtained from ATCC (U934 and Thp1) or AllCells (CD36 selection; 99%pure). Purified NK, T, and DC cells were from AllCells (99%pur | Full Paper |
2019 | Morgan, H;Tseng, SY;Gallais, Y;Leineweber, M;Buchmann, P;Riccardi, S;Nabhan, M;Lo, J;Gani, Z;Szely, N;Zhu, CS;Yang, M;Kiessling, A;Vohr, HW;Pallardy, M;Aswad, F;Turbica, I; | Evaluation of in vitro Assays to Assess the Modulation of Dendritic Cells Functions by Therapeutic Antibodies and Aggregates | Frontiers in immunology | 31001248 | Wuppertal (Germany). For each site, healthy donors gave their written consent for the use of blood donation for research purposes. Also, PBMC directly isolated and frozen were purchased from vendor (AllCells, CA, USA). In each partner lab's, PBMC is | Full Paper |
2019 | Saul, L;Mair, I;Ivens, A;Brown, P;Samuel, K;Campbell, JDM;Soong, DY;Kamenjarin, N;Mellanby, RJ; | 1,25-Dihydroxyvitamin D3 Restrains CD4+ T Cell Priming Ability of CD11c+ Dendritic Cells by Upregulating Expression of CD31 | Frontiers in immunology | 30984180 | SA as per manufacturer's instructions (eBioscience, San Diego, USA). Generation of Human DC From Mobilized Peripheral Blood CD34+ Progenitor Cells CD34+ cells were obtained from a commercial source (AllCells, LLC., Alameda, CA, USA). The CD34+ cells | Full Paper |
2019 | Smith, AJ;Miller, SM;Buhl, C;Child, R;Whitacre, M;Schoener, R;Ettenger, G;Burkhart, D;Ryter, K;Evans, JT; | Species-Specific Structural Requirements of Alpha-Branched Trehalose Diester Mincle Agonists | Frontiers in immunology | 30873180 | PBMCs were obtained via leukapheresis from normal donors (AllCells, Berkeley, CA) or fresh blood was obtained from health human donors through a University of Montana Save | Full Paper |
2019 | Du, Y;Montoya, C;Orrego, S;Wei, X;Ling, J;Lelkes, PI;Yang, M; | Topographic cues of a novel bilayered scaffold modulate dental pulp stem cells differentiation by regulating YAP signalling through cytoskeleton adjustments | Cell proliferation | 31424140 | ilometer (Surfcorder SE 1700, Kosaka Labs) with a 2 μm radius tip, a spread of 2.5 mm and a speed of 0.5 mm/s. 2.2 Dental pulp stem cells culture on the scaffolds Human DPSCs were purchased from AllCells LLC. These cells were guaranteed by company t | Full Paper |
2019 | Cunin, P;Bouslama, R;Machlus, KR;Martínez-Bonet, M;Lee, PY;Wactor, A;Nelson-Maney, N;Morris, A;Guo, L;Weyrich, A;Sola-Visner, M;Boilard, E;Italiano, JE;Nigrovic, PA; | Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to platelets | eLife | 31042146 | and Schulze, 2005). Fetal liver-derived MKs were generated as described (Machlus et al., 2017). Human MK generation: mobilized peripheral blood or BM CD34 +stem/progenitor cells were purchased from AllCells. 1 × 105 cells were cultured in StemSpan me | Full Paper |
2019 | Dura-Bernal, S;Suter, BA;Gleeson, P;Cantarelli, M;Quintana, A;Rodriguez, F;Kedziora, DJ;Chadderdon, GL;Kerr, CC;Neymotin, SA;McDougal, RA;Hines, M;Shepherd, GM;Lytton, WW; | NetPyNE, a tool for data-driven multiscale modeling of brain circuits | eLife | 31025934 | ode to disk file and combines these files after the simulation has ended. After gathering, the built-in analysis functions have direct access to all the network and simulation output data via sim.net.allCells and sim.allSimData. Importing and export | Full Paper |
2019 | Frank, ND;Jones, ME;Vang, B;Coeshott, C; | Evaluation of reagents used to coat the hollow-fiber bioreactor membrane of the Quantum® Cell Expansion System for the culture of human mesenchymal stem cells | Materials science & engineering. C, Materials for biological applications | 30606590 | Healthy, fresh, adult human whole bone marrow (WBM) was purchased either from AllCells LLC (Alameda, CA) or from Lonza (Basel, Switzerland). Flow cytometry staining buffer was Save | Full Paper |
2019 | Laner-Plamberger, S;Oeller, M;Mrazek, C;Hartl, A;Sonderegger, A;Rohde, E;Strunk, D;Schallmoser, K; | Upregulation of mitotic bookmarking factors during enhanced proliferation of human stromal cells in human platelet lysate | Journal of translational medicine | 31888679 | ed from white adipose tissue (WAT) and umbilical cord (UC) after signed informed consent by the donors as described previously [26, 30, 31]. Bone marrow (BM)-derived stromal cells were purchased from AllCells, (Alameda, CA, USA, http://www.allcells.c | Full Paper |
2019 | Antebi, B;Asher, AM;Rodriguez, LA;Moore, RK;Mohammadipoor, A;Cancio, LC; | Cryopreserved mesenchymal stem cells regain functional potency following a 24-h acclimation period | Journal of translational medicine | 31464641 | Human MSCs were isolated from fresh bone-marrow mononuclear cells purchased from AllCells (Alameda, CA). The MSCs were expanded in complete culture media (CCM) consisting Save | Full Paper |
2019 | Coeshott, C;Vang, B;Jones, M;Nankervis, B; | Large-scale expansion and characterization of CD3+ T-cells in the Quantum® Cell Expansion System | Journal of translational medicine | 31391068 | g and unique dual-loop circuits. Materials and methods Preparation of peripheral blood mononuclear cells (PBMCs) Peripheral blood leukapheresis products were obtained from individual healthy donors (AllCells, LLC, Alameda, CA, HemaCare Corp, Van Nuy | Full Paper |
2019 | Shi, L;Zhong, Z;Li, X;Zhou, Y;Pan, Z; | Discovery of an Orally Available Janus Kinase 3 Selective Covalent Inhibitor | Journal of medicinal chemistry | 30615446 | Frozen PBMCs (from Allcells) were thawed in RPMI1640 (Thermo Fisher) containing 10% FBS and recovered overnight at 37 °C. The next day, cells were diluted to 1 × 106 cells/mL and seeded (500 μL) in 6-well plates. Compounds or DMSO (5 μL, serially | Full Paper |
2019 | Zoine, JT;Knight, KA;Fleischer, LC;Sutton, KS;Goldsmith, KC;Doering, CB;Spencer, HT; | Ex vivo expanded patient-derived γδ T-cell immunotherapy enhances neuroblastoma tumor regression in a murine model | Oncoimmunology | 31413905 | Commercially available healthy donor frozen PBMCs were obtained from AllCells (Alameda, CA). At the time of stem cell collection, each patient had undergone two cycles of induction Save | Full Paper |
2019 | Rodriguez, LA;Mohammadipoor, A;Alvarado, L;Kamucheka, RM;Asher, AM;Cancio, LC;Antebi, B; | Preconditioning in an Inflammatory Milieu Augments the Immunotherapeutic Function of Mesenchymal Stromal Cells | Cells | 31096722 | D) for immune- and/or inflammatory-mediated diseases. 2. Materials and Methods 2.1. Isolation of BM-MSCs and AD-MSCs Human BM-MSCs were isolated from commercially available mononuclear cells (MNCs) (AllCells LLC; Emeryville, CA, USA), as previously | Full Paper |
2019 | Youn, M;Huang, H;Chen, C;Kam, S;Wilkes, MC;Chae, HD;Sridhar, KJ;Greenberg, PL;Glader, B;Narla, A;Lin, S;Sakamoto, KM; | MMP9 inhibition increases erythropoiesis in RPS14-deficient del(5q) MDS models through suppression of TGF-β pathways | Blood advances | 31540902 | Primary human CD34 + hematopoietic stem/progenitor cells were purified from bone marrow aspirates purchased from Allcells and Stemcell Technologies. CD34 + cells were purified Save | Full Paper |
2019 | Li, C;Mathews, V;Kim, S;George, B;Hebert, K;Jiang, H;Li, C;Zhu, Y;Keesler, DA;Boelens, JJ;Dvorak, CC;Agarwal, R;Auletta, JJ;Goyal, RK;Hanna, R;Kasow, K;Shenoy, S;Smith, AR;Walters, MC;Eapen, M; | Related and unrelated donor transplantation for β-thalassemia major: results of an international survey | Blood advances | 31471325 | Full Paper | |
2019 | Yang, HM;Choi, JJ;Kim, HN;Yang, SJ;Park, SJ;Kang, C;Chung, HM;Lee, MR;Kim, SJ;Moon, SH; | Reconstituting Human Cutaneous Regeneration in Humanized Mice under Endothelial Cell Therapy | The Journal of investigative dermatology | 30393080 | The derivation of CB-EPCs was performed from CB mononuclear cells, which were purchased from AllCells (Alameda, CA). Briefly, CB mononuclear cells were seeded at a density of 4-6 × 106/mL in a collagen-coated tissue culture dish and cultured in EGM-2 | Full Paper |
2019 | Jeng, MY;Mumbach, MR;Granja, JM;Satpathy, AT;Chang, HY;Chang, ALS; | Enhancer Connectome Nominates Target Genes of Inherited Risk Variants from Inflammatory Skin Disorders | The Journal of investigative dermatology | 30315781 | Briefly, donor human peripheral blood cells were obtained from AllCells (Emeryville, CA) and were sorted based on the following markers: CD4 + CD25 − CD45RA + (naive), CD4 + Save | Full Paper |
2019 | Naipauer, J;Rosario, S;Gupta, S;Premer, C;Méndez-Solís, O;Schlesinger, M;Ponzinibbio, V;Jain, V;Gay, L;Renne, R;Chan, HL;Morey, L;Salyakina, D;Abba, M;Williams, S;Hare, JM;Goldschmidt-Clermont, PJ;Mesri, EA; | PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment | PLoS pathogens | 31881074 | n SCA-1; and (iii) the ability to differentiate into adipocytes or osteoblast-like cells. Human MSCs were isolated as described previously [39]. Briefly, BM aspirates (25-50 mL) were purchased from AllCells (Emeryville, CA) under appropriate informed | Full Paper |
2019 | Bi, W;Xu, W;Cheng, L;Xue, J;Wang, Q;Yu, F;Xia, S;Wang, Q;Li, G;Qin, C;Lu, L;Su, L;Jiang, S; | IgG Fc-binding motif-conjugated HIV-1 fusion inhibitor exhibits improved potency and in vivo half-life: Potential application in combination with broad neutralizing antibodies | PLoS pathogens | 31805154 | Env (2.5 × 104 cells) as the target cells were seeded into a 96-well plate (25 μL/well), and the mixture of a serially diluted inhibitors and 10E8 (1.5 μg/mL) was added to the culture plate. PBMC (AllCells) as effector cells (2.5 × 104 cells in 25 μL | Full Paper |
2019 | Wang, W;Liu, T;Yang, L;Ma, Y;Dou, F;Shi, L;Wen, A;Ding, Y; | Study on the multi-targets mechanism of triphala on cardio-cerebral vascular diseases based on network pharmacology | Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie | 31112872 | Triphala was obtained from Tibet Jinzhu Along Tibetan Medicine Co. Ltd. (Tibet, China). The Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from AllCells (Emeryville, CA, USA). Primary antibodies against β-actin, PTGS2, MMP9 and IL6 we | Full Paper |
2019 | Acosta, SA;Lee, JY;Nguyen, H;Kaneko, Y;Borlongan, CV; | Endothelial Progenitor Cells Modulate Inflammation-Associated Stroke Vasculome | Stem cell reviews and reports | 30739275 | linded to the treatment condition. Cell Culture Procedures EPC Differentiation from Human Bone Marrow Mesenchymal Stem Cells Frozen human bone marrow CD34+ mesenchymal stem cells were purchased from ALLCELLS (Catalog# ABM017F; Alameda, CA). After th | Full Paper |
2019 | Meehan, S;Kolyagin, GA;Parks, D;Youngyunpipatkul, J;Herzenberg, LA;Walther, G;Ghosn, EEB;Orlova, DY; | Automated subset identification and characterization pipeline for multidimensional flow and mass cytometry data clustering and visualization | Communications biology | 31240267 | Fresh bone marrow mononuclear cells from adult healthy donors were obtained commercially from AllCells, LLC (Quincy, MA, Cat # ABM024). Data was collected for about 0.5-1.0 × 10 Save | Full Paper |
2019 | Guo, X;Yuan, Z;Xu, Y;Zhao, X;Fang, Z;Yuan, WE; | A Low-Molecular-Weight Polyethylenimine/pDNA-VEGF Polyplex System Constructed in a One-Pot Manner for Hindlimb Ischemia Therapy | Pharmaceutics | 30965617 | Aldrich. VEGF-A (1239 bp) pDNA encoding GFP was constructed by Bioroot Biology (Shanghai, China). 2.2. Cell Culture Human umbilical vein endothelial cells (HUVECs, primary cells) were purchased from ALLCELLS (Shanghai, China) and cultured in RPMI-16 | Full Paper |
2019 | Zhang, M;Xu, Y;Jiang, L; | Irisin attenuates oxidized low-density lipoprotein impaired angiogenesis through AKT/mTOR/S6K1/Nrf2 pathway | Journal of cellular physiology | 30942905 | Commercial HUVECs were obtained from Allcells (Shanghai, China). Cells were seeded in DMEM/F12 medium with 20% FBS (Gibco), 60 μg/ml endothelial cell growth supplement (BD, San Diego, CA) and maintained at 37°C in a CO2 incubator. The HMEC-1 cells we | Full Paper |
2019 | Wang, J;Fei, K;Jing, H;Wu, Z;Wu, W;Zhou, S;Ni, H;Chen, B;Xiong, Y;Liu, Y;Peng, B;Yu, D;Jiang, H;Liu, J; | Durable blockade of PD-1 signaling links preclinical efficacy of sintilimab to its clinical benefit | mAbs | 31402780 | × MSD read buffer with surfactant (R92TC-1, MSD) was added into each well. The electrochemiluminescent signal was then measured on the MSD SQ120 instrument. In vitro PD-1 receptor occupancy PBMCs (AllCells, Alameda, California, USA) were activated b | Full Paper |
2019 | Ovacik, AM;Li, J;Lemper, M;Danilenko, D;Stagg, N;Mathieu, M;Ellerman, D;Gupta, V;Kalia, N;Nguy, T;Plaks, V;David Johnson, C;Wang, W;Brumm, J;Fine, B;Junttila, T;Lin, K;Carter, PJ;Prabhu, S;Spiess, C;Kamath, AV; | Single cell-produced and in vitro-assembled anti-FcRH5/CD3 T-cell dependent bispecific antibodies have similar in vitro and in vivo properties | mAbs | 30550367 | 100 (number of live target cells without TDB - number of live target cells with TDB)/number of live target cells without TDB. Human BM aspirates from healthy donors (n = 2, male) (procured from AllCells, ABM001-1) were diluted in phosphate-buffered | Full Paper |
2019 | Wang, H;He, H;Yang, C; | miR-342 suppresses the proliferation and invasion of acute myeloid leukemia by targeting Naa10p | Artificial cells, nanomedicine, and biotechnology | 31496296 | The MNC normal control samples were isolated from normal BM cells purchased from AllCells, LLC (Emeryville, CA) by use of NycoPrep 1.077 A (Axis-Shield, Oslo, Norway) according Save | Full Paper |
2019 | Chang, AY;Hsu, E;Patel, J;Li, Y;Zhang, M;Iguchi, H;Rogoff, HA; | Evaluation of Tumor Cell-Tumor Microenvironment Component Interactions as Potential Predictors of Patient Response to Napabucasin | Molecular cancer research : MCR | 31043490 | pSTAT3 is a potential biomarker for patient response to the anticancer drug napabucasin. Visual Overview: http://mcr.aacrjournals.org/content/molcanres/17/7/1429/F1.large.jpg . Save | Full Paper |
2019 | Marklein, RA;Klinker, MW;Drake, KA;Polikowsky, HG;Lessey-Morillon, EC;Bauer, SR; | Morphological profiling using machine learning reveals emergent subpopulations of interferon-γ-stimulated mesenchymal stromal cells that predict immunosuppression | Cytotherapy | 30503100 | Human bone marrow-derived MSCs were obtained from 11 different donors purchased from Lonza and AllCells at passage two (see Supplementary Table 1 for donor specifications). A Save | Full Paper |
2019 | Sei, J;Moses, B;Becker, A;Kim, M;Kaur, N;Vemuri, M;Civin, C; | StemPro HSC expansion medium (Prototype) supports superior expansion of human hematopoietic stem-progenitor cells | Cytotherapy | Single donor, cord-blood derived, CD34+ cells obtained from AllCells were thawed and cultured in CTS StemPro HSC expansion media containing SCF, FLT3, TPO, IL3 and IL6 growth Save | Full Paper | |
2019 | Kou, X;Sun, Y;Li, S;Bian, W;Liu, Z;Zhang, D;Jiang, J; | Pharmacology Study of the Multiple Angiogenesis Inhibitor RC28-E on Anti-Fibrosis in a Chemically Induced Lung Injury Model | Biomolecules | 31652997 | The human skin fibroblast cell line Detroit 551 was purchased from AllCells (Shanghai, China) and maintained in MEM medium (Gibco, Grand Island, NY, USA) supplemented with 10% Save | Full Paper |
2019 | Zhang, Y;Yu, S;Tuazon, JP;Lee, JY;Corey, S;Kvederis, L;Kingsbury, C;Kaneko, Y;Borlongan, CV; | Neuroprotective effects of human bone marrow mesenchymal stem cells against cerebral ischemia are mediated in part by an anti-apoptotic mechanism | Neural regeneration research | 30632499 | Bcl-2 antibody was 58, 117, or 235 ng/mL. Both hMSC and Bcl-2 doses were based on pilot studies demonstrating their potencies. Cryopreserved human bone marrow CD34+ cells (hBM34+) were purchased from AllCells (Alameda, CA, USA). The exposure time of | Full Paper |
2019 | Weisgrau, KL;Vosler, LJ;Pomplun, NL;Hayes, JM;Simmons, HA;Friedrichs, KR;Rakasz, EG; | Neutrophil progenitor populations of rhesus macaques | Journal of leukocyte biology | 30395351 | We purchased de-identified, freshly obtained human bone marrow and matching blood samples from 18-65 years old healthy individuals from AllCells LLC (Boston, USA), or Lonza Inc. (Mapleton, IL, USA). The donors signed a procedure-specific consent form | Full Paper |
2019 | Cao, L;Weetall, M;Trotta, C;Cintron, K;Ma, J;Kim, MJ;Furia, B;Romfo, C;Graci, JD;Li, W;Du, J;Sheedy, J;Hedrick, J;Risher, N;Yeh, S;Qi, H;Arasu, T;Hwang, S;Lennox, W;Kong, R;Petruska, J;Moon, YC;Babiak, J;Davis, TW;Jacobson, A;Almstead, NG;Branstrom, A;Colacino, JM;Peltz, SW; | Targeting of Hematologic Malignancies with PTC299, A Novel Potent Inhibitor of Dihydroorotate Dehydrogenase with Favorable Pharmaceutical Properties | Molecular cancer therapeutics | 30352802 | AML cells, purchased from AllCells (January 2017), Inc and Champions Oncology, Inc (April 2017), were cultured in StemSpan medium (Stemcell Technologies, Vancouver). Cell lines in culture were routinely verified once per month to be mycoplasma free a | Full Paper |
2019 | Boothello, RS;Patel, NJ;Sharon, C;Abdelfadiel, EI;Morla, S;Brophy, DF;Lippman, HR;Desai, UR;Patel, BB; | A Unique Nonsaccharide Mimetic of Heparin Hexasaccharide Inhibits Colon Cancer Stem Cells via p38 MAP Kinase Activation | Molecular cancer therapeutics | 30337351 | The cells were resuspended in 10 mL of Iscove's modified Dulbecco's medium (2% FBS media; Allcells) and mixed with Methyl cellulose (R&D systems), and 1.1 mL of the final cell Save | Full Paper |
2019 | Suhito, IR;Kang, ES;Kim, DS;Baek, S;Park, SJ;Moon, SH;Luo, Z;Lee, D;Min, J;Kim, TH; | High density gold nanostructure composites for precise electrochemical detection of human embryonic stem cells in cell mixture | Colloids and surfaces. B, Biointerfaces | 31082776 | For the comparison, cryopreserved hCB-EPCs were purchased from AllCells, LLC (California, USA) [26,27]. The hCB-EPCs were thawed in a 37 °C warm bath for 2 min and washed twice with 100 μl DNase (Sigma-Aldrich) in 10 ml PBS. Cells were cultured on a | Full Paper |
2019 | Xu, P;Ganaie, SS;Wang, X;Wang, Z;Kleiboeker, S;Horton, NC;Heier, RF;Meyers, MJ;Tavis, JE;Qiu, J; | Endonuclease Activity Inhibition of the NS1 Protein of Parvovirus B19 as a Novel Target for Antiviral Drug Development | Antimicrobial agents and chemotherapy | 30530599 | Primary human CD34+ hematopoietic stem cells (HSCs) were isolated from the bone marrow of a healthy human donor. We purchased them from AllCells LLC (Alameda, CA), and B19V-containng plasma samples were obtained from ViraCor Eurofins Laboratories (Le | Full Paper |
2019 | Patel, PR;Senyuk, V;Rodriguez, NS;Oh, AL;Bonetti, E;Mahmud, D;Barosi, G;Mahmud, N;Rondelli, D; | Synergistic Cytotoxic Effect of Busulfan and the PARP Inhibitor Veliparib in Myeloproliferative Neoplasms | Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation | 30615982 | Normal CD34 + cells used as healthy controls were obtained from apheresis collection or cord blood (AllCells, Alameda, CA). Mononuclear cells were subsequently obtained by Save | Full Paper |
2019 | Campos, JM;Sousa, AC;Caseiro, AR;Pedrosa, SS;Pinto, PO;Branquinho, MV;Amorim, I;Santos, JD;Pereira, T;Mendonça, CM;Afonso, A;Atayde, LM;Maurício, AC; | Dental pulp stem cells and Bonelike® for bone regeneration in ovine model | Regenerative biomaterials | 30740242 | onmental SEM with X-ray microanalysis and electron backscattered diffraction analysis: Quanta 400 FEG ESEM/EDAX Genesis X4M . hMSC preparation Cell culture and maintenance hDPSCs were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01) | Full Paper |
2019 | Dai, L;Hu, W;Yang, Z;Chen, D;He, B;Chen, Y;Zhou, L;Xie, H;Wu, J;Zheng, S; | Upregulated expression of HOXB7 in intrahepatic cholangiocarcinoma is associated with tumor cell metastasis and poor prognosis | Laboratory investigation; a journal of technical methods and pathology | 30664713 | Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Shanghai, China; allcells.biomart.cn) and maintained in DMEM supplemented with 10% fetal bovine Save | Full Paper |
2019 | Sachdeva, M;Duchateau, P;Depil, S;Poirot, L;Valton, J; | Granulocyte-macrophage colony-stimulating factor inactivation in CAR T-cells prevents monocyte-dependent release of key cytokine release syndrome mediators | The Journal of biological chemistry | 30804212 | Cryopreserved human PBMCs were acquired from ALLCELLS (catalog no. PB006F), and human monocytes were acquired from STEMCELL Technologies (catalog no. 70035.1). Both PBMCs and monocytes were cultured in X-vivo-15 media (Lonza, catalog no. BE04-418Q), | Full Paper |
2019 | Yamamoto, R;Xu, Y;Ikeda, S;Sumida, K;Tanaka, H;Hozumi, K;Takaori-Kondo, A;Minato, N; | Thymic Development of a Unique Bone Marrow-Resident Innate-like T Cell Subset with a Potent Innate Immune Function | Journal of immunology (Baltimore, Md. : 1950) | 31085589 | Human BM mononuclear cells from healthy donors were purchased from AllCells (Alameda, CA). T cells, B cells, myeloid cells, and dendritic cells were isolated from BM cells by Save | Full Paper |
2019 | Nikitin, PA;Rose, EL;Byun, TS;Parry, GC;Panicker, S; | C1s Inhibition by BIVV009 (Sutimlimab) Prevents Complement-Enhanced Activation of Autoimmune Human B Cells In Vitro | Journal of immunology (Baltimore, Md. : 1950) | 30635392 | Primary PBMC from de-identified patients with rheumatoid arthritis were purchased from AllCells. No therapeutic modalities were used as exclusion criteria for samples from patients Save | Full Paper |
2019 | Wu, J;Chen, L;Wang, R;Song, Z;Shen, Z;Zhao, Y;Huang, S;Lin, Z; | Exosomes Secreted by Stem Cells from Human Exfoliated Deciduous Teeth Promote Alveolar Bone Defect Repair through the Regulation of Angiogenesis and Osteogenesis | ACS biomaterials science & engineering | 33405738 | (FBS; Gibco), 100 U/mL penicillin G, as well as 100 mg/mL streptomycin. HUVECs were bought from ALLCELLS (Shanghai, China) and expanded in endothelial cell growth medium (EGM). BMSCs were harvested from the femoral bones of SD rats. All experimental | Full Paper |
2019 | Huang, YJ;Lee, CY;Cao, J;Lee, HS;Chang, CH;Chen, PD;Wu, YM; | Therapeutic Potential of Plasma Proteins Derived from Umbilical Cord Blood for Acute Liver Failure | Molecular pharmaceutics | 30698974 | There are very limited clinically viable treatment options for acute liver failure, a life-threatening condition that rapidly progresses to loss of liver function. In this study, we aim to evaluate Save | Full Paper |
2019 | Seo, A;Gulsuner, S;Pierce, S;Ben-Harosh, M;Shalev, H;Walsh, T;Krasnov, T;Dgany, O;Doulatov, S;Tamary, H;Shimamura, A;King, MC; | Inherited thrombocytopenia associated with mutation of UDP-galactose-4-epimerase (GALE) | Human molecular genetics | 30247636 | CD34+ cells from human cord blood were purchased from AllCells. Thawed cells were expanded for 4 h at 37C in tissue culture incubator with X-VIVO10 media (Lonza) supplemented Save | Full Paper |
2019 | Moore, N;Chevillet, JR;Healey, LJ;McBrine, C;Doty, D;Santos, J;Teece, B;Truslow, J;Mott, V;Hsi, P;Tandon, V;Borenstein, JT;Balestrini, J;Kotz, K; | A Microfluidic Device to Enhance Viral Transduction Efficiency During Manufacture of Engineered Cellular Therapies | Scientific reports | 31641163 | Both Research Blood Components and AllCells obtain IRB approved consent from all donors giving permission to collect their blood or blood product and use or sell it for research Save | Full Paper |
2019 | Fischer, CL;Bates, AM;Lanzel, EA;Guthmiller, JM;Johnson, GK;Singh, NK;Kumar, A;Vidva, R;Abbasi, T;Vali, S;Xie, XJ;Zeng, E;Brogden, KA; | Computational Models Accurately Predict Multi-Cell Biomarker Profiles in Inflammation and Cancer | Scientific reports | 31350446 | experiments, GE KER were suspended at 1 × 105 viable cells/ml in Lymphocyte Growth Medium-3 (LGM-3, Lonza Walkersville, Inc., Walkersville, MD). Human monocyte-derived immature myeloid DC (DC, AllCells, Alameda, CA) were positively identified by flo | Full Paper |
2019 | Laner-Plamberger, S;Oeller, M;Poupardin, R;Krisch, L;Hochmann, S;Kalathur, R;Pachler, K;Kreutzer, C;Erdmann, G;Rohde, E;Strunk, D;Schallmoser, K; | Heparin Differentially Impacts Gene Expression of Stromal Cells from Various Tissues | Scientific reports | 31076619 | ed by the ethical committee of federal state of Salzburg. All donors of BM, WAT and UC signed an informed consent concerning the research use of materials. Additionally, BM samples were obtained from AllCells, (Alameda, CA, USA, www.allcells.com/stat | Full Paper |
2019 | Yea, K;Jones, TM;Jung, D;Shin, S;Arlian, BM;Han, KH;Zha, Z;Kim, MS;Oh, YS;Zhang, H;Lerner, RA; | An agonist antibody prefers relapsed AML for induction of cells that kill each other | Scientific reports | 30837591 | population studied here is much larger than that simply defined by CD34+ and CD38+ cells. Methods Culture of AML cells Human AML cells were isolated from patients and frozen at −80 °C (ABM010, AllCells). The informed consent was obtained from all pa | Full Paper |
2019 | Das, P;Panda, SK;Agarwal, B;Behera, S;Ali, SM;Pulse, ME;Solomkin, JS;Opal, SM;Bhandari, V;Acharya, S; | Novel Chitohexaose Analog Protects Young and Aged mice from CLP Induced Polymicrobial Sepsis | Scientific reports | 30814582 | mg/kg to young adult mice and 25 mg/kg to aged mice in a 0.2 mL volume near the dorsal scapular region. Cell lines Human peripheral blood mononuclear cells (HPBMCs) were purchased from LeukoPaK (AllCells); 0.5 × 106 cells/well were seeded in 96-well | Full Paper |
2019 | Kizilyer, A;Singh, MV;Singh, VB;Suwunnakorn, S;Palis, J;Maggirwar, SB; | Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces Thrombopoiesis | Scientific reports | 30808933 | CD34+ cells derived from BM or umbilical cord blood were purchased from Allcells (Emeryville, CA, USA), and differentiated as previously described 48 . For inducing MK progenitor ( Save | Full Paper |
2019 | Zarriello, S;Neal, EG;Kaneko, Y;Borlongan, CV; | T-Regulatory Cells Confer Increased Myelination and Stem Cell Activity after Stroke-Induced White Matter Injury | Journal of clinical medicine | 31010132 | Purified BMSCs were obtained and stored in aliquots in dry ice (AllCells, Almeda, CA, USA). After the cells reached confluence, they were subjected to an oxygen-glucose deprivation Save | Full Paper |
2019 | Hu, B;Zou, Y;Zhang, L;Tang, J;Niedermann, G;Firat, E;Huang, X;Zhu, X; | Nucleofection with Plasmid DNA for CRISPR/Cas9-Mediated Inactivation of Programmed Cell Death Protein 1 in CD133-Specific CAR T Cells | Human gene therapy | 29706119 | Frozen peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from ALLCELLS (PB005F). The tumor cell lines used in this study were generated based on a Save | Full Paper |
2019 | Bednar, KJ;Nycholat, CM;Rao, TS;Paulson, JC;Fung-Leung, WP;Macauley, MS; | Exploiting CD22 To Selectively Tolerize Autoantibody Producing B-Cells in Rheumatoid Arthritis | ACS chemical biology | 30835424 | Frozen peripheral blood mononuclear cells (PBMCs) were obtained through AllCells (Alameda, CA) or BioreclamationIVT (Chestertown, MD) as per institutional protocols for human sample acquisition. Cells were thawed in accordance with the manufacture’s | Full Paper |
2019 | Schott, JW;León-Rico, D;Ferreira, CB;Buckland, KF;Santilli, G;Armant, MA;Schambach, A;Cavazza, A;Thrasher, AJ; | Enhancing Lentiviral and Alpharetroviral Transduction of Human Hematopoietic Stem Cells for Clinical Application | Molecular therapy. Methods & clinical development | 31338385 | lus HSC Brew GMP supplement (Miltenyi Biotec, Surrey, UK) additionally supplemented with 2% human albumin. For the GMP experiment, HD CD34+ HSPCs were isolated from a fresh mobilized leukapheresis (AllCells, Alameda, CA, USA) following standard immu | Full Paper |
2019 | Ellison, SM;Liao, A;Wood, S;Taylor, J;Youshani, AS;Rowlston, S;Parker, H;Armant, M;Biffi, A;Chan, L;Farzaneh, F;Wynn, R;Jones, SA;Heal, P;Gaspar, HB;Bigger, BW; | Pre-clinical Safety and Efficacy of Lentiviral Vector-Mediated Ex Vivo Stem Cell Gene Therapy for the Treatment of Mucopolysaccharidosis IIIA | Molecular therapy. Methods & clinical development | 31044143 | CD34 + cells were positively selected from two pooled PB mononuclear cell (PBMC) apheresis units (Allcells, USA) using the Miltenyi CliniMACSPlus instrument and reagents Save;00 for a further 24 hrs prior to seeding in CFU assay. Large-Scale Trans | Full Paper |
2019 | Liu, W;Zhang, C;Wang, L;Huang, X;Zhang, J;He, Y;Chen, L;Li, J; | Successful reversal of ovarian hyperstimulation syndrome in a mouse model by rapamycin, an mTOR pathway inhibitor | Molecular human reproduction | 31329230 | HUVEC were purchased from ALLCELLS and seeded on micropore inserts (pore size 0.4 μm, Corning) in a density of 3 × 10 5 cells per insert and cultured overnight with or without 40 Save | Full Paper |
2019 | Cheng, Y;Dai, X;Yang, T;Zhang, N;Liu, Z;Jiang, Y; | Low Long Noncoding RNA Growth Arrest-Specific Transcript 5 Expression in the Exosomes of Lung Cancer Cells Promotes Tumor Angiogenesis | Journal of oncology | 31186629 | ells were purchased from the Chinese Academy of Sciences Type Culture Collections Cell Bank (Shanghai, China). The primary human umbilical vein endothelial cells (HUVECs) were purchased from Shanghai AllCells Biotechnology Company (Shanghai, China). | Full Paper |
2019 | Kim, CH;Lim, CY;Lee, JH;Kim, KC;Ahn, JY;Lee, EJ; | Human Embryonic Stem Cells-Derived Mesenchymal Stem Cells Reduce the Symptom of Psoriasis in Imiquimod-Induced Skin Model | Tissue engineering and regenerative medicine | 30815354 | The PBMCs were purchased from AllCells, LLC. The hE-MSCs were inactivated by treatment with 10 μg/mL mitomycin-C (Sigma-Aldrich, St. Louis, MO, USA) for 1 h at 37 C. Save | Full Paper |
2019 | Geldenhuys, WJ;Nair, RR;Piktel, D;Martin, KH;Gibson, LF; | The MitoNEET Ligand NL-1 Mediates Antileukemic Activity in Drug-Resistant B-Cell Acute Lymphoblastic Leukemia | The Journal of pharmacology and experimental therapeutics | 31010844 | Primary CD3 + T cells, CD19 + B cells, peripheral blood mononuclear cells, and bone marrow mononuclear cells were purchased from AllCells (Allcells.com) and maintained in Save | Full Paper |
2019 | Xu, L;Zhao, G;Zhu, H;Wang, S;Sun, A;Zou, Y;Ge, J; | Peroxisome Proliferator-Activated Receptor-γ Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5p | PPAR research | 31354796 | Quantitative analysis of stained lipid area was performed by a blinded observer using Leica QWin V3 software. 2.3. In Vitro HUVECs Culture and Cell Immunostaining Primary HUVECs were purchased from AllCells (Shanghai, China) and cultured in low-gluc | Full Paper |
2019 | Zhao, S;Molina, A;Yu, A;Hanson, J;Cheung, H;Li, X;Natkunam, Y; | High frequency of CD74 expression in lymphomas: implications for targeted therapy using a novel anti-CD74-drug conjugate | The journal of pathology. Clinical research | 30191677 | egression analysis with one site‐specific binding equation on GraphPad Prism (La Jolla, CA, USA). Whole bone marrow (BM) aspirates (3 ml each) from five healthy human donors was obtained through AllCells, Inc. (Alameda, CA, USA). FACS binding on BM | Full Paper |
2019 | Xia, F;Yang, C;Wang, Y;Youcef-Toumi, K;Reuter, C;Ivanov, T;Holz, M;Rangelow, IW; | Lights Out! Nano-Scale Topography Imaging of Sample Surface in Opaque Liquid Environments with Coated Active Cantilever Probes | Nanomaterials (Basel, Switzerland) | 31337145 | Immersion test for whole blood sample with Ethylenediaminetetraacetic acid (EDTA) anti-coagulation treatment from AllCells LLC was also conducted. The coated probe worked well at Save | Full Paper |
2019 | Yu, Y;Yoo, SM;Park, HH;Baek, SY;Kim, YJ;Lee, S;Kim, YL;Seo, KW;Kang, KS; | Preconditioning with interleukin-1 beta and interferon-gamma enhances the efficacy of human umbilical cord blood-derived mesenchymal stem cells-based therapy via enhancing prostaglandin E2 secretion and indoleamine 2,3-dioxygenase activity in dextran sulfate sodium-induced colitis | Journal of tissue engineering and regenerative medicine | 31293088 | 2.7 Isolation of CD4+ T cells with magnetic-activated cell sorting (MACS) CD4+ T cells were purified from PBMCs (ALLCells, Alameda, CA, USA) using MACS (Miltenyi Biotec, Bergisch Gladbach, Germany) as previously discribed (Hong et al., 2017) | Full Paper |
2019 | Yin, Y;Guo, J;Teng, F;Yu, L;Jiang, Y;Xie, K;Jiang, M;Fang, J; | Preparation of a Novel One-Armed Anti-c-Met Antibody with Antitumor Activity Against Hepatocellular Carcinoma | Drug design, development and therapy | 31849449 | bovine serum (FBS, qualified, Australia#10099-141; Gibco Co., Ltd) in a humidified incubator in the presence of 5% CO2 at 37°C. Human umbilical vein endothelial cells (HUVECs) were obtained from the Allcells Co., Ltd. (Shanghai, China). The cells wer | Full Paper |
2019 | Kono, M;Matsuhiroya, S;Nakazawa, F;Kaido, M;Wada, A;Tomiyama, Y; | Morphological and optical properties of human immature platelet-enriched population produced in immunodeficient mice | Platelets | 30059642 | The animals were fed a pellet chow and sterilized tap water ad libitum. Human CD34-positive cells (1 × 105) from cord blood (AllCells, CA, USA) were transplanted through the tail vein after irradiation with 2.5 Gy. Eltrombopag at 25 mg/kg body weight | Full Paper |
2019 | Gangolli, RA;Devlin, SM;Gerstenhaber, JA;Lelkes, PI;Yang, M; | A Bilayered Poly (Lactic-Co-Glycolic Acid) Scaffold Provides Differential Cues for the Differentiation of Dental Pulp Stem Cells | Tissue engineering. Part A | 29984629 | Human DPSCs were purchased from AllCells LLC (Emeryville, CA), and cultured in a basal medium comprised of 85% MEM Alpha (Gibco ThermoFisher Scientific, Waltham, MA) supplemented with 15% fetal bovine serum (Stem Cell qualified; ThermoFisher Scientif | Full Paper |
2019 | Huang, L;Tan, J;Li, W;Zhou, L;Liu, Z;Luo, B;Lu, L;Zhou, C; | Functional polyhedral oligomeric silsesquioxane reinforced poly(lactic acid) nanocomposites for biomedical applications | Journal of the mechanical behavior of biomedical materials | 30500698 | The sterilization step of samples was similar to above films. HUVECs (purchased from Allcells, shanghai, China) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin (P/S, Gibco) | Full Paper |
2019 | Cackowski, FC;Wang, Y;Decker, JT;Sifuentes, C;Weindorf, S;Jung, Y;Wang, Y;Decker, AM;Yumoto, K;Szerlip, N;Buttitta, L;Pienta, KJ;Morgan, TM;Taichman, RS; | Detection and isolation of disseminated tumor cells in bone marrow of patients with clinically localized prostate cancer | The Prostate | 31449673 | Normal marrow samples were purchased from AllCells (Alameda, CA), drawn from the posterior superior iliac crest of paid donors, transferred to heparinized tubes and shipped Save | Full Paper |
2019 | Zheng, Q;Zhang, X;Yang, H;Xie, J;Xie, Y;Chen, J;Yu, C;Zhong, C; | Internal Ribosome Entry Site Dramatically Reduces Transgene Expression in Hematopoietic Cells in a Position-Dependent Manner | Viruses | 31597367 | anassas, VA, USA). Human embryonic kidney cell line 293T was purchased from the Institute of Biochemistry Cell Biology (Shanghai, China). The CD34+ hematopoietic stem cells (HSCs) were purchased from ALLCELLS (Alameda, CA, USA). The hepatocellular ca | Full Paper |
2019 | Zhao, H;Chen, L;Zhong, G;Huang, Y;Zhang, X;Chu, C;Chen, L;Wang, M; | Titanium Dioxide Nanoparticles Induce Mitochondrial Dynamic Imbalance and Damage in HT22 Cells | Journal of Nanomaterials | HT22 cells were from AllCells (Shanghai Biological Technology Co. Ltd., Shanghai, China). We carried out some experiments in the BE(2)C cell line, which is a human neuroblastoma cell line. The cells were planted in Dulbecco’s minimum essential medium | Full Paper | |
2019 | Liang, YR;Zhang, T;Jia, P;Xu, XL;Fang, Y;Ding, XQ; | Interaction between bone marrow-derived dendritic cells and miR-21 of tubular renal epithelial cells under hypoxia | European review for medical and pharmacological sciences | 30840288 | cluster of differentiation 86), MHCII (major histocompatibility complex class II molecules), and CCR7 (eBioscience, Frankfurt, Germany). Primary Murine Renal TEC Culture Primary renal TECs were bought from AllCells (Allcells, LLC, Berkeley, CA, USA) | Full Paper |
2019 | Youngblood, BA;Brock, EC;Leung, J;Falahati, R;Bryce, PJ;Bright, J;Williams, J;Shultz, LD;Greiner, DL;Brehm, MA;Bebbington, C;Tomasevic, N; | AK002, a Humanized Sialic Acid-Binding Immunoglobulin-Like Lectin-8 Antibody that Induces Antibody-Dependent Cell-Mediated Cytotoxicity against Human Eosinophils and Inhibits Mast Cell-Mediated Anaphylaxis in Mice | International archives of allergy and immunology | 31401630 | Purified human natural killer (NK) cells (AllCells) were washed with culture medium and added at 450,000 cells/well (effector: target cell ratio 9:1). Plates were incubated for 4 h at 37C Save | Full Paper |
2019 | Gautam, A;Donohue, D;Hoke, A;Miller, SA;Srinivasan, S;Sowe, B;Detwiler, L;Lynch, J;Levangie, M;Hammamieh, R;Jett, M; | Investigating gene expression profiles of whole blood and peripheral blood mononuclear cells using multiple collection and processing methods | PloS one | 31809517 | and the PAXgene blood RNA tubes were immediately frozen, one set of triplicate EDTA tubes was stored at 4C, and the remaining tubes received further processing at the AllCells, Save | Full Paper |
2019 | Caseiro, AR;Santos Pedrosa, S;Ivanova, G;Vieira Branquinho, M;Almeida, A;Faria, F;Amorim, I;Pereira, T;Maurício, AC; | Mesenchymal Stem/ Stromal Cells metabolomic and bioactive factors profiles: A comparative analysis on the umbilical cord and dental pulp derived Stem/ Stromal Cells secretome | PloS one | 31774816 | ngiogenesis. 2. Materials and methods 2.1. Human Mesenchymal Stem/ Stromal Cells characterization and conditioning protocol Umbilical Cord -MSCs [PromoCellTM (C-12971; Lot No. 1112304.2)] and DPSCs [AllCells, LLC (DP0037F, Lot No. DPSC090411-01)] we | Full Paper |
2019 | Manshouri, T;Verstovsek, S;Harris, DM;Veletic, I;Zhang, X;Post, SM;Bueso-Ramos, CE;Estrov, Z; | Primary myelofibrosis marrow-derived CD14+/CD34- monocytes induce myelofibrosis-like phenotype in immunodeficient mice and give rise to megakaryocytes | PloS one | 31569199 | nstitutional Review Board (MDACC IRB1) approved written informed consent had been obtained. The patients’ clinical data are provided in S1 Table. As controls, normal BM aspirates were obtained from AllCells (Alameda, CA). Low-density cells were fract | Full Paper |
2019 | Singer, JW;Al-Fayoumi, S;Taylor, J;Velichko, S;O'Mahony, A; | Comparative phenotypic profiling of the JAK2 inhibitors ruxolitinib, fedratinib, momelotinib, and pacritinib reveals distinct mechanistic signatures | PloS one | 31560729 | eline Cell Technologies and Cell Applications. Peripheral blood mononuclear cells (PBMCs) and positively selected primary normal human CD19+ B cells, CD4+ T cells, and CD14+ monocytes were purchased (AllCells, Emeryville, CA) or were isolated in-hous | Full Paper |
2019 | Gunawardena, TNA;Masoudian, Z;Rahman, MT;Ramasamy, TS;Ramanathan, A;Abu Kasim, NH; | Dental derived stem cell conditioned media for hair growth stimulation | PloS one | 31042749 | ulp stem cells obtained from human deciduous teeth (SHED) in comparison to CM produced using HFSCs. Methodology Cell culturing of SHED and HFSCs SHED and HFSCs Cells at Passage 1 were purchased from AllCells, USA and ICELLTIS, Labege, France respect | Full Paper |
2019 | Mamontov, P;Eberwine, RA;Perrigoue, J;Das, A;Friedman, JR;Mora, JR; | A negative role for the interleukin-2-inducible T-cell kinase (ITK) in human Foxp3+ TREG differentiation | PloS one | 31022269 | or pharmacological targeting of specific Tec kinases in inflammatory and autoimmune disease. Materials and methods Cell culture Naïve CD4+ CD45RA+ CD25- peripheral blood T cells were purchased from AllCells (Alameda, CA). For Th0 non-polarized condi | Full Paper |
2019 | Rytlewski, J;Deng, S;Xie, T;Davis, C;Robins, H;Yusko, E;Bienkowska, J; | Model to improve specificity for identification of clinically-relevant expanded T cells in peripheral blood | PloS one | 30870493 | expansion, have broad clinical value. Methods Training cohort Whole blood samples collected from 10 healthy donors at three time points with 2-week intervals between collections were purchased from AllCells, LLC (Emeryville, CA). Among the 10 donors | Full Paper |
2019 | Yoshida, M;Horiguchi, H;Kikuchi, S;Iyama, S;Ikeda, H;Goto, A;Kawano, Y;Murase, K;Takada, K;Miyanishi, K;Kato, J;Kobune, M; | miR-7977 inhibits the Hippo-YAP signaling pathway in bone marrow mesenchymal stromal cells | PloS one | 30835743 | r127; ab76252) and anti-NF2/Merlin Ab (ab88957) were purchased from Abcam (Tokyo, Japan). Human BM CD34+ cells and three different lot numbers of MSCs from healthy volunteers (HV) were purchased from AllCells, LLC (Toronto, Canada) (HV-derived MSCs # | Full Paper |
2019 | Chung, JE;Magis, W;Vu, J;Heo, SJ;Wartiovaara, K;Walters, MC;Kurita, R;Nakamura, Y;Boffelli, D;Martin, DIK;Corn, JE;DeWitt, MA; | CRISPR-Cas9 interrogation of a putative fetal globin repressor in human erythroid cells | PloS one | 30645582 | based on mobility using a separate FASC hemoglobin standard (Trinity biotech). Electroporation of CD34+ HSPCs with Cas9 RNPs HSPC electroporation was as described [28]. CD34+ HSPCs (G-CSF mobilized, Allcells, Inc.) were thawed and cultured in SFEM ( | Full Paper |
2019 | Hu, X;Shen, G;Hu, T;Guan, G;Wang, L; | Use of an in vitro dynamic culture system to assess flow shear forces upon cell adhesion within different structures | Journal of Chemical Technology & Biotechnology | Human umbilical vein endothelial cells (HUVEC001), provided by Allcells Company (Shanghai, China), were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, South American) and 1% penicillin-streptomycin (Gib | Full Paper | |
2019 | Satani, N;Giridhar, K;Cai, C;Wewior, N;Norris, DD;Olson, SD;Aronowski, J;Savitz, SI; | Aspirin in stroke patients modifies the immunomodulatory interactions of marrow stromal cells and monocytes | Brain research | 31220426 | MSCs from fresh human bone marrow aspirates (AllCells, Alameda, CA) were prepared from 3 separate healthy volunteers using density centrifugation and plastic adherence as previously described (Diaz et al., 2017, Kota et al., 2017, Parsha et al., 2017 | Full Paper |
2019 | Mohd Nor, NH;Berahim, Z;Azlina, A;Kannan, TP; | Identification of novel fibroblast-like cells from stem cells from human exfoliated deciduous teeth | Clinical oral investigations | 30847574 | Objectives This study aimed to differentiate and characterize fibroblast-like cells from stem cells from human exfoliated deciduous teeth (SHED). Materials and methods The Save | Full Paper |
2019 | McIntyre, PW;Wu, JL;Kolte, R;Zhang, R;Gregory, RL;Bruzzaniti, A;Yassen, GH; | The antimicrobial properties, cytotoxicity, and differentiation potential of double antibiotic intracanal medicaments loaded into hydrogel system | Clinical oral investigations | 29943369 | Human dental pulp stem cells (DPSC) (AllCells, Alameda, CA, USA) were cultured in 24-well plates (Cat: TP9024, Alkali Scientific Inc.) at concentrations of 3 × 104 cells/well in α-MEM (HyClone Laboratories Inc., South Logan, UT, USA) with 10% heat-in | Full Paper |
2019 | Zhong, X;Gao, W;Wu, R;Liu, H;Ge, J; | Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanism | Molecular medicine reports | 31638185 | HUVECs were obtained from AllCells, LLC and cultured in ECM (Scien Cell Research Laboratories, Inc.). The 8-10th generations of HUVECs were used in this study and they were harvested for mRNA or protein detection after treatment with exosomes for 6 o | Full Paper |
2019 | Chen, T;Li, T;Wang, J; | p53 mediates PEDF‑induced autophagy in human umbilical vein endothelial cells through sestrin2 signaling | Molecular medicine reports | 31173218 | ed to investigate the association between the PEDF-p53-sestrin pathway and autophagy in human umbilical vein endothelial cells (HUVECs). Materials and methods Cell culture HUVECs were purchased from AllCells (Alameda, CA, USA). Cells were cultured i | Full Paper |
2019 | Feitosa, SA;Palasuk, J;Geraldeli, S;Windsor, LJ;Bottino, MC; | Physicochemical and biological properties of novel chlorhexidine-loaded nanotube-modified dentin adhesive | Journal of biomedical materials research. Part B, Applied biomaterials | 30199597 | The cytotoxicity of the CHX-loaded nanotube-modified adhesive and control groups (SBMP and HNT) were evaluated using human dental pulp stem cells (hDPSC, AllCells, Alameda, Save | Full Paper |
2019 | Kim, MM;Schlussel, L;Zhao, L;Himburg, HA; | Dickkopf-1 Treatment Stimulates Hematopoietic Regenerative Function in Infused Endothelial Progenitor Cells | Radiation research | 31081743 | Human bone marrow CD34+ cells (AllCells®, Alameda, CA) were irradiated at 3 Gy and added in noncontact to the upper portion of the transwell at a dose of 10,000 cells/well. Cells were cultured for 7 days, viable cells were enumerated by trypan blue e | Full Paper |
2019 | Choi, HW;Chan, C;Shterev, ID;Lynch, HE;Robinette, TJ;Johnson-Weaver, BT;Shi, J;Sempowski, GD;Kim, SY;Dickson, JK;Gooden, DM;Abraham, SN;Staats, HF; | Identification of Novel Mast Cell Activators Using Cell-Based High-Throughput Screening | SLAS discovery : advancing life sciences R & D | 30917061 | Primary human mast cells were differentiated from human CD34+ hematopoietic stem cells.19 Human CD34+ hematopoietic stem cells were obtained from commercial sources (Allcells). Isolated CD34+ cells was suspended at 5 × 105 cells/mL in IMDM medium sup | Full Paper |
2019 | Patel, A;Clementelli, CM;Jarocha, D;Mosoyan, G;Else, C;Kintali, M;Fong, H;Tong, J;Gordon, R;Gillespie, V;Keyzner, A;Poncz, M;Hoffman, R;Iancu-Rubin, C; | Pre-clinical development of a cryopreservable megakaryocytic cell product capable of sustained platelet production in mice | Transfusion | 31802511 | Division of Hematology and Medical Oncology, Tisch Cancer Institute and the Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York.++Division of Hematology and Medical Oncology, Tisch Cancer Institute and the Department o | Full Paper |
2019 | Md Hashim, S;Yusof, M;Zahari, W;Chandra, H;Ahmad Amin Noordin, K;Kannan, T;Sheikh Abdul Hamid, S;Mokhtar, K;Azlina, A; | Human Amniotic Membrane as a Matrix for Endothelial Differentiation of VEGF-Treated Dental Stem Cells | Cellular and Molecular Bioengineering | SHED (AllCells, USA) were cultured in the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, USA) supplemented with 15% of fetal bovine serum (FBS) (Gibco, USA) and 50 U/mL of penicillin-streptomycin (Gibco, USA). The cells were incubated in | Full Paper | |
2019 | Juillerat, A;Tkach, D;Busser, BW;Temburni, S;Valton, J;Duclert, A;Poirot, L;Depil, S;Duchateau, P; | Modulation of chimeric antigen receptor surface expression by a small molecule switch | BMC biotechnology | 31269942 | witching-off CAR T-cells. Overall, this could mitigate some of the toxicities that could occur with early intense antitumor responses. Methods T-cell proliferation Cryopreserved human PBMCs/T-cells (ALLCELLS, cat # PB006F) were used in accordance wi | Full Paper |
2019 | Kim, OH;Yoon, OJ;Lee, HJ; | Silk fibroin scaffolds potentiate immunomodulatory function of human mesenchymal stromal cells | Biochemical and biophysical research communications | 31506179 | Although mesenchymal stromal cells (MSCs) show great potential for use in regenerative medicine, their therapeutic efficacy remains limited because of their low adaptation efficiency Save | Full Paper |
2019 | Luo, H;Hu, L;Ma, B;Zhao, M;Luo, M;Deng, Q;Deng, S;Ye, H;Lin, T;Chen, J;Wang, T;Zhu, J;Lu, H; | Molecular dynamics based improvement of the solubilizing self-cleavable tag Zbasic-ΔI-CM application in the preparation of recombinant proteins in Escherichia coli | Biochemical and biophysical research communications | 30967267 | HUVEC was obtained from ATCC (Cat# CRL-1730) and cultured in ECM medium supplemented with 5% fetal bovine serum and 1 × ECGS (Allcells; Shanghai, China). For proliferation detection, the cells were harvested and re-suspended using the assay medium (E | Full Paper |
2019 | Camarillo, JM;Swaminathan, S;Abshiru, NA;Sikora, JW;Thomas, PM;Kelleher, NL; | Coupling Fluorescence-Activated Cell Sorting and Targeted Analysis of Histone Modification Profiles in Primary Human Leukocytes | Journal of the American Society for Mass Spectrometry | 31286445 | De-identified donor peripheral blood (PB) specimen were obtained from AllCells (Alameda, CA), Innovative Research (Novi, MI), or as clinical discards from the Clinical Flow Cytometry Laboratory at Northwestern Memorial Hospital. All procedures perfor | Full Paper |
2019 | Ishikawa, G;Fujiwara, N;Hirschfield, H;Varricchio, L;Hoshida, Y;Barosi, G;Rosti, V;Padilla, M;Mazzarini, M;Friedman, SL;Hoffman, R;Migliaccio, AR; | Shared and Tissue-Specific Expression Signatures between Bone Marrow from Primary Myelofibrosis and Essential Thrombocythemia | Experimental hematology | 31678370 | Bone marrow from eight healthy individuals was purchased from AllCells Technology (Oakland, CA). mRNA in amounts sufficient for analyses was obtained from 6 PMF and all ET Save | Full Paper |
2019 | Will, B;Kao, Y;Chen, J;Aivalioti, M;Tatiparthy, M; | 1029 - IRON HOMEOSTASIS-REGULATORY PATHWAYS IN HEMATOPOIETIC STEM CELLS | Experimental Hematology | Healthy cryopreserved human bone marrow cells and CD34+ enriched cells were purchased from AllCells (Alameda, CA) and ReachBio Research Labs (ReachBio LLC, WA). Fresh unprocessed bone marrow cells were purchased from Lonza (Lonza, MD), and the cells | Full Paper | |
2019 | Yang, H;Sun, J;Chen, H;Wang, F;Li, Y;Wang, H;Qu, T; | Mesenchymal stem cells from bone marrow attenuated the chronic morphine-induced cAMP accumulation in vitro | Neuroscience letters | 30605704 | Human bone marrow (n = 3) was purchased from AllCells Inc (Emeryville, CA). MSCs were isolated from the bone marrow according to previously described methods [16]. In brief, the bone marrow was diluted 1:1 with DMEM (low glucose, Invitrogen, CA, USA) | Full Paper |
2019 | Muruganandham, A;Dumont, C;Xing, K; | Approaching stability challenges for flow cytometry in a regulated bioanalytical environment | Bioanalysis | 31637937 | Mobilized human whole blood, with K 3 EDTA as anticoagulant, was purchased from ALLCELLS . Celerion stabilizer was added at 20% volume of blood within 24 h of collection and Save | Full Paper |
2019 | Choi, SA;An, JH;Lee, SH;Lee, GH;Yang, HJ;Jeong, PS;Cha, JJ;Lee, S;Park, YH;Song, BS;Sim, BW;Kim, YH;Kim, JS;Jin, YB;Huh, JW;Lee, SR;Lee, JH;Kim, SU; | Comparative Evaluation of Hormones and Hormone-Like Molecule in Lineage Specification of Human Induced Pluripotent Stem Cells | International journal of stem cells | 31242719 | mising other lineage decisions. Materials and Methods Cell culture, hiPSC generation, and lineage specification To obtain hiPSCs, CD34+ cells were purified from primary human bone marrow (BM) cells (Allcells, Emeryville, CA, USA) via immunomagnetic | Full Paper |
2019 | Kuramitsu, M;Okuma, K;Tezuka, K;Nakamura, H;Sagara, Y;Kurane, I;Hamaguchi, I; | Development and evaluation of human T-cell leukemia virus-1 and -2 multiplex quantitative PCR | Microbiology and immunology | 31429972 | Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells Inc. (Alameda, CA), TL-Om1 cells have been reported previously 15 and the MoT cell line (CRL-8066) was purchased from ATCC (Manassas, VA). Plasmid DNA pX1MT-M,16 an HTLV-1 molecu | Full Paper |
2019 | Li, X;Li, X;Lin, J;Sun, X;Ding, Q; | Exosomes Derived From Low-Intensity Pulsed Ultrasound-Treated Dendritic Cells Suppress Tumor Necrosis Factor-Induced Endothelial Inflammation | Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine | 30561085 | Human Umbilical Vein Endothelial Cell Culture Human umbilical vein endothelial cells were purchased from AllCells and cultured in endothelial cell medium (ScienCell, Shanghai, China) in a humidified atmosphere of 95% air and 5% CO2 at 37°C | Full Paper |
2019 | Choi, JH;Necsoiu, C;Wendorff, D;Jordan, B;Dixon, A;Roberts, TR;Beely, BM;Cancio, LC;Batchinsky, AI; | Effects of adjunct treatments on end-organ damage and histological injury severity in acute respiratory distress syndrome and multiorgan failure caused by smoke inhalation injury and burns | Burns : journal of the International Society for Burn Injuries | 31378621 | The process of disqualifying AssureImmune and qualifying AllCells as a new source of bone marrow for the production of MSCs delayed our timeline. 2. Based on our published Phase Save | Full Paper |
2019 | Ma, J;Lai, G;Lu, Z; | Effect of 410 nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor (VEGF) in cultured human vascular endothelial cells | Lasers in medical science | 30350123 | dilution with RPMI-1640 medium (ALLCELLS). Working drug solutions (0-8 μg/ml) were prepared by diluting the stock solution with RPMI-1640 medium (ALLCELLS. CA, USA). A light- Save | Full Paper |
2019 | Miura, S;Kobune, M;Horiguchi, H;Kikuchi, S;Iyama, S;Murase, K;Goto, A;Ikeda, H;Takada, K;Miyanishi, K;Kato, J; | EPO-R+ myelodysplastic cells with ring sideroblasts produce high erythroferrone levels to reduce hepcidin expression in hepatic cells | Blood cells, molecules & diseases | 31082798 | Total RNA derived from human BM CD34+, CB CD34+ and human mesenchymal stromal (MSCs) cells was purchased from AllCells, LLC (Toronto, Canada). Human myeloid leukemia Save | Full Paper |
2019 | Li, Y;Zhao, R;Li, X;Wang, C;Bao, H;Wang, S;Fang, J;Huang, J;Wang, C; | Blood-compatible Polyaniline Coated Electrospun Polyurethane Fiber Scaffolds for Enhanced Adhesion and Proliferation of Human Umbilical Vein Endothelial Cells | Fibers and Polymers | prothrombin time agent were purchased from AllCells Biological Technology Co., Ltd. Phosphate buffered saline (PBS) was purchased from Beijing Dingguo Changsheng Save | Full Paper | |
2019 | Yamasaki, AE;King, NE;Matsui, H;Jepsen, K;Panopoulos, AD; | Two iPSC lines generated from the bone marrow of a relapsed/refractory AML patient display normal karyotypes and myeloid differentiation potential | Stem cell research | 31739201 | 7q and 13q, were obtained from AllCells, LLC. Immunohistochemistry analysis of the patient's a normal karyotype (both analyses provided by AllCells, LLC). Previously published iPSCs Save | Full Paper |
2019 | CECHIM, G;CHIES, J; | In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive review | Anais da Academia Brasileira de Ciências | Commercial leukopaks generally contain 80-200 mL of processed material with approximately 7x109peripheral blood mononuclear cells (PBMC), (more information available on: www.allcells.com/products/whole-tissue/leuko-pak). The Table I shows the percent | Full Paper | |
2019 | Coronado, RE;Somaraki-Cormier, M;Ong, JL;Halff, GA; | Hepatocyte-like cells derived from human amniotic epithelial, bone marrow, and adipose stromal cells display enhanced functionality when cultured on decellularized liver substrate | Stem cell research | 31163390 | Bone Marrow Derived Mesenchymal Stromal/Stem Cells: Bone marrow derived mesenchymal stem cells (BM-MSCs) were isolated from whole bone marrow aspirates (AllCells Alameda Save | Full Paper |
2019 | Abhyankar, VP;Bates, AM;Fischer, CL;Johnson, GK;Guthmiller, JM;Progulske-Fox, A;Brogden, KA; | Dataset on the chemokine and cytokine responses of multi-cell cultures treated with Porphyromonas gingivalis hemagglutinin B | Data in brief | 30740479 | HagB (QCL-1000, Chromogenic Limulus Amebocyte Lysate Assay, Lonza, Inc. Walkersville, MD USA). 2.2 Dendritic cells, GE keratinocytes, and T cells Non-peripheral blood human myeloid dendritic cells (AllCells, LLC, Alameda, CA USA) were thawed and sus | Full Paper |
2019 | Hii, SC;Luddin, N;Kannan, TP;Ab Rahman, I;Nik Abdul Ghani, NR; | The Biological Evaluation of Conventional and Nano-Hydroxyapatite-Silica Glass Ionomer Cement on Dental Pulp Stem Cells: A Comparative Study | Contemporary clinical dentistry | 32308298 | DPSCs purchased from AllCells, USA were used in this study. DPSCs were cultured in Alpha Minimum Essential Medium (α-MEM) (Gibco, Life Technologies, USA) supplemented with Save | Full Paper |
2019 | Akers, S; | Modeling central nervous system involvement in acute lymphoblastic leukemia | West Virginia University Libraries | Scientific Corporation, Westbury, NY) according to the manufacturer’s instructions. Positively selected peripheral blood CD19+ B-cells from a healthy donor were purchased from AllCells (Emeryville, CA). Human brain-derived microvascular endothelial c | Full Paper | |
2019 | Barve, A; | Characterization of a more clinically relevant human leukemia xenograft model to examine perturbation of MET/SAM metabolism as a novel therapeutic paradigm for MLL-R leukemia in vivo. | University of Louisville | Acute myeloid leukemia (AML), is a heterogeneous clonal disorder characterized by an accumulation of malignant myeloid progenitors in the bone marrow (BM), hindering normal Save | Full Paper | |
2019 | Liu, H;Kezios, S; | DYNAMIC CHARACTERISTICS OF ETTINGSHAUSEN REFRIGERATOR | Proceeding of International Heat Transfer Conference 4 | However this was not the case in allcells and in cells from plant 41, with 3 accessory chromo¬somes, sometimes none, one, two or all three accessorychromosomes divided precociously Save | Full Paper | |
2019 | Rodriguez, L;Mohammadipoor, A;Kamucheka, R;Asher, A;Alvarado, L;Antebi, B;Cancio, L; | Preconditioning in an Acute Respiratory Distress Syndrome-Like Milieu Augments the Immunomodulatory and Anti-Inflammatory Function of MSCS | A60. LUNG INJURY, SEPSIS, AND ARDS | Methods: Human bone marrow (BM)-MSCs were isolated from fresh mononuclear cells purchased from AllCells (Alameda, CA). Human adipose-derived (AD)-MSCs were isolated from Save | Full Paper | |
2019 | De Maio, V;Brandic, I; | Multi-Objective Mobile Edge Provisioning in Small Cell Clouds | Proceedings of the 2019 ACM/SPEC International Conference on Performance Engineering | Also, we can observe significant losses when using the other two algorithms, ALLCELLS and RANDOM. In the first case this is because the algorithm overestimates the number of Edge Save | Full Paper | |
2019 | Lal, A;Chiang, Z;Yakovenko, N;Duarte, F;Israeli, J;Buenrostro, J; | AtacWorks: A deep convolutional neural network toolkit for epigenomics | bioRxiv | Cryopreserved human bone marrow mononuclear cells were purchased from Allcells (catalog number BM, CR, MNC, 10M). Cells were quickly thawed in a 37°C water bath, rinsed with culture medium (RPMI 1640 medium supplemented with 15% FBS) and | Full Paper | |
2019 | Liu, J;Kozhaya, L;Torres, V;Unutmaz, D;Lu, M; | Structure-Based Discovery of a Novel Small-Molecule Inhibitor of Methicillin-Resistant S. aureus | bioRxiv | Blood samples were obtained from healthy, consenting donors as Buffy coats (New York Blood Center) and leukopaks (AllCells, Alameda, CA). PBMCs were isolated from peripheral blood by density gradient centrifugation using Ficoll-Paque | Full Paper | |
2019 | Wang, X;Derakhshandeh, R;Rodriguez, H;Han, D;Kostyushev, D;Henry, T;Traverse, J;Moyé, L;Simari, R;Taylor, D;Springer, M; | Impaired Therapeutic Efficacy of Bone Marrow Cells from Post-Myocardial Infarction Patients in the TIME and LateTIME Clinical Trials | bioRxiv | Healthy de-identified donor BM MNCs were purchased from AllCells (Alameda, CA). These MNCs were manually isolated at AllCells using Ficoll conditions like that in the Sepax Save | Full Paper | |
2019 | Granja, J;Klemm, S;McGinnis, L;Kathiria, A;Mezger, A;Parks, B;Gars, E;Liedtke, M;Zheng, G;Chang, H;Majeti, R;Greenleaf, W; | A single cell framework for multi-omic analysis of disease identifies malignant regulatory signatures in mixed phenotype acute leukemia | bioRxiv | PBMCs, BMMCs, and CD34+ bone marrow cells were obtained from healthy donors (AllCells). Description of Leukemic Patients/Donors Patient samples were collected with informed consent prospectively under a protocol approved by the Institutional Review | Full Paper | |
2019 | Ding, J;Adiconis, X;Simmons, S;Kowalczyk, M;Hession, C;Marjanovic, N;Hughes, T;Wadsworth, M;Burks, T;Nguyen, L;Kwon, J;Barak, B;Ge, W;Kedaigle, A;Carroll, S;Li, S;Hacohen, N;Rozenblatt-Rosen, O;Shalek, A;Villani, A;Regev, A;Levin, J; | Systematic comparative analysis of single cell RNA-sequencing methods | bioRxiv | We acquired commercially-available PBMCs (AllCells; Lot #PB006F-A5865) as frozen aliquots with 25 million cells for the PBMC1 experiment or isolated them from fresh blood (AllCells Save | Full Paper | |
2019 | Fan, L;Zhao, M; | Gold nanorod based delivery system could bring about superior therapy effect of Ramucirumab through direct cytotoxicity to cancer cell mediated by differential regulation of phagocytosis in gastric cancer cell | bioRxiv | HUVEC cell was purchased from Allcells (Endothelial Cell Medium, H-004B, Allcells, supplemented with 5%fetal bovine serum), GC cells SNU-5 (1640 Cell Medium, Gibco, supplemented with 10% fetal bovine serum) cell was purchased from ATCC and MKN | Full Paper | |
2019 | King, GR; | Comparative systemic analysis of human immunoglobulin repertoires | Thesis | All peripheral blood and bone marrow aspirate samples were collected by AllCells and shipped overnight at ambient temperature; processing was started immediately upon receipt of the samples in a sterilized biological safety hood. Sterile 1x PBS pH 7. | Full Paper | |
2019 | Sakhnevych, S; | Tim-3-galectin-9 immunosuppressive pathway in human acute myeloid leukaemia and solid tumour cells and biochemical functions of its crucial components | Thesis | Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordance with the manufacturer's instructions following ethical approval (REC reference: 16-SS-033). Other p | Full Paper | |
2019 | Lee, MJ; | The Development of Novel Proteasome Inhibitors for the Treatment of Multiple Myeloma and Alzheimer's Disease | Thesis | Over a decade, proteasome inhibitors (PIs), bortezomib, carfilzomib (Cfz) and ixazomib, have contributed to a significant improvement in the overall survival for multiple myeloma (MM) Save | Full Paper | |
2019 | Kuhlman, BM; | MICROGRAVITY NEGATIVELY AFFECTS DNA DAMAGE REPAIR AND THE ANTI-LEUKEMIC FUNCTION OF HUMAN NATURAL KILLER CELLS | Thesis | Healthy human bone marrow (BM) mononuclear cells (BMMNC) were purchased from AllCells, LLC (Alameda, CA, USA). CD34+ HSC were isolated from BMMNC using antihuman CD34 MicroBeads and the MiniMACS magnetic-based positive-selection system (Miltenyi Biot | Full Paper | |
2019 | 龚子祯, ;蔡海波, ;谭文松, ; | 黄原胶支持 CIK 细胞高密度扩增 | 高校化学工程学报 | RPMI 1640购于美国Gibco公司; 血清培养基(自配);PBMC购于上海Allcells公司;CD3单克隆 抗体购于美国eBioscience公司;植物血凝 PHA购于美国Sigma-Aldrich公司;干扰 (IFN-γ),白介 Save | Full Paper | |
2019 | Cao, YP;Pan, M;Song, YL;Zhang, HL; | MiR-302 a/b/c suppresses tumor angiogenesis in hepatocellular carcinoma by targeting MACC1 | European Review for Medical and Pharmacological Sciences | The umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Huh7 and PLC/PRF/5 cells were incubated with Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine ser | Full Paper | |
2019 | 王一唯, ;黄婷, ;孙笑, ;王玉东, ; | RANKL 募集调节性 T 细胞促进子宫内膜癌侵袭的研究 | 中国免疫学杂志 | 科院细胞库),外周单个 细胞(美国ALLCELLS 公 司),RANK 慢病毒(上海和元生物科技公司),重组 人TGF鄄茁,重组人RANKL(美国Peprotech 公司),人 RANKL ELISA 试剂盒(武汉博士德生物 Save | Full Paper | |
2019 | Alvarado, L; | Evaluating the Therapeutic Effects of Mesenchymal Stem Cells in an in Vitro Co-Culture System of a Damaged Alveolar Barrier | Thesis | Human BM-MSCs were isolated from commercially available MNCs (AllCells LLC; Emeryville, CA). Bone marrow specimen were obtained from human donors 20-30 years of age. MNCs were cultured on standard culture flasks at a density of 3 x 105 cells/cm2 in | Full Paper | |
2019 | Romero-Masters, J; | Studying Epstein-Barr virus-induced lymphomagenesis in a cord-blood humanized mouse model | Thesis | Creation of cord blood-humanized NOD/LtSz-scid/IL2Rγnull mice: Immunodeficient NSG (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson labs (catalogue number: 005557). Commercially available CD34-depleted human cord blood mononuclear cells (Al | Full Paper | |
2019 | Mumbach, MR; | Technologies for Protein and RNA-Focused Chromosome Conformation | Thesis | Normal donor human peripheral blood cells were obtained fresh from AllCells. CD4+ T cells were enriched from peripheral blood using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). For CD4+ T helper cell subtypes, Naïve T c | Full Paper | |
2019 | Walker, KL; | Non-chemotherapeutic Methods of Treating T Cell Acute Lymphoblastic Leukemia | Thesis | Chapter 4.2: EOpansion of NK cell products Fresh leukopaks were commercially o9tained (AllCells, Alameda, CA) from paid human apheresis donors, stored at 1Г10o C, and Save | Full Paper | |
2019 | Srivastava, S;Salter, AI;Liggitt, D;Yechan-Gunja, S;Sarvothama, M;Cooper, K;Smythe, KS;Dudakov, JA;Pierce, RH;Rader, C;Riddell, SR; | Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor Targeting | Cancer cell | 30889382 | CFU Assays Femurs and tibia from control and ROR1 CAR-T cell-treated mice were harvested 9 days post-T cell transfer and submitted to ReachBio Inc. for CFU-GM, BFU-E, CFU-Mk, Pre-B CFC, and CFU-F assays | Full Paper |
2019 | Humbert, O;Radtke, S;Samuelson, C;Carrillo, RR;Perez, AM;Reddy, SS;Lux, C;Pattabhi, S;Schefter, LE;Negre, O;Lee, CM;Bao, G;Adair, JE;Peterson, CW;Rawlings, DJ;Scharenberg, AM;Kiem, HP; | Therapeutically relevant engraftment of a CRISPR-Cas9-edited HSC-enriched population with HbF reactivation in nonhuman primates | Science translational medicine | 31366580 | For CFC assays, 1000-1200 sorted cells were seeded into 3.5 ml ColonyGEL 1402 (ReachBio). Hematopoietic colonies were scored after 12-14 days. Arising colonies were identified as colony-forming unit- (CFU-) granulocyte (CFU-G), macrophage | Full Paper |
2019 | Crawford, A;Haber, L;Kelly, MP;Vazzana, K;Canova, L;Ram, P;Pawashe, A;Finney, J;Jalal, S;Chiu, D;Colleton, CA;Garnova, E;Makonnen, S;Hickey, C;Krueger, P;DelFino, F;Potocky, T;Kuhnert, J;Godin, S;Retter, MW;Duramad, P;MacDonald, D;Olson, WC;Fairhurst, J;Huang, T;Martin, J;Lin, JC;Smith, E;Thurston, G;Kirshner, JR; | A Mucin 16 bispecific T cell-engaging antibody for the treatment of ovarian cancer | Science translational medicine | 31217340 | Regeneron Pharmaceuticals Institutional Animal Care and Use Committee. NSG (20 to 24 weeks old) mice (the Jackson Laboratory) were injected with 5 × 106 human PBMCs (ReachBio) before 2 × 106 ascites cells from the OVCAR-3/Luc cell line, | Full Paper |
2019 | Li, C;Mishra, AS;Gil, S;Wang, M;Georgakopoulou, A;Papayannopoulou, T;Hawkins, RD;Lieber, A; | Targeted Integration and High-Level Transgene Expression in AAVS1 Transgenic Mice after In Vivo HSC Transduction with HDAd5/35++ Vectors | Molecular therapy : the journal of the American Society of Gene Therapy | 31494053 | 2,500 Lin− cells were plated in triplicates in ColonyGEL 1202 mouse complete medium (ReachBio, Seattle, WA) and incubated for 12 days at 37°C in 5% CO2 and maximum humidity. Colonies were enumerated using a Leica MS 5 dissection microscope (Leica Mic | Full Paper |
2019 | Kuhlmann, AS;Haworth, KG;Barber-Axthelm, IM;Ironside, C;Giese, MA;Peterson, CW;Kiem, HP; | Long-Term Persistence of Anti-HIV Broadly Neutralizing Antibody-Secreting Hematopoietic Cells in Humanized Mice | Molecular therapy : the journal of the American Society of Gene Therapy | 30391142 | erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor (GM-CSF). At necropsy, cells isolated from bone marrow (BM) were seeded in triplicate into ColonyGEL (ReachBio, Seattle WA). Hem | Full Paper |
2019 | Humbert, O;Laszlo, GS;Sichel, S;Ironside, C;Haworth, KG;Bates, OM;Beddoe, ME;Carrillo, RR;Kiem, HP;Walter, RB; | Engineering resistance to CD33-targeted immunotherapy in normal hematopoiesis by CRISPR/Cas9-deletion of CD33 exon 2 | Leukemia | 30291334 | system (Harvard Apparatus, Cambridge, MA) [12]. For evaluation of colony-forming units (CFUs), 1500 CD34+ cells were seeded in 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA) and scored after 12-14 days. CFU DNA was extracted in QuickExtract (Epicentre | Full Paper |
2019 | Ly, M;Rentas, S;Vujovic, A;Wong, N;Moreira, S;Xu, J;Holzapfel, N;Bhatia, S;Tran, D;Minden, MD;Draper, JS;Hope, KJ; | Diminished AHR Signaling Drives Human Acute Myeloid Leukemia Stem Cell Maintenance | Cancer research | 31519687 | Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio), supplemented with DMSO or 750 Save | Full Paper |
2019 | Smitheman, KN;Severson, TM;Rajapurkar, SR;McCabe, MT;Karpinich, N;Foley, J;Pappalardi, MB;Hughes, A;Halsey, W;Thomas, E;Traini, C;Federowicz, KE;Laraio, J;Mobegi, F;Ferron-Brady, G;Prinjha, RK;Carpenter, CL;Kruger, RG;Wessels, L;Mohammad, HP; | Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes | Haematologica | 30514804 | uated for each assay. Primary acute myeloid leukemia blast colony Clonogenic progenitors from primary AML patients’ samples were assessed in a semi-solid methylcellulose-based medium (ColonyGel , ReachBio) containing recombinant human (rh) SCF (50 ng | Full Paper |
2019 | Fuller, NO;Pirone, A;Lynch, BA;Hewitt, MC;Quinton, MS;McKee, TD;Ivarsson, M; | CoREST Complex-Selective Histone Deacetylase Inhibitors Show Prosynaptic Effects and an Improved Safety Profile To Enable Treatment of Synaptopathies | ACS chemical neuroscience | 30496686 | Normal human bone marrow light density cells derived from normal bone marrow (NorCal Biologics, California) and qualified at ReachBio were stored in the gaseous phase of liquid Save;minimum peptide length of at least 7 amino acids was required. Ef | Full Paper |
2019 | Decker, CE;Young, T;Pasnikowski, E;Chiu, J;Song, H;Wei, Y;Thurston, G;Daly, C; | Genome-scale CRISPR activation screen uncovers tumor-intrinsic modulators of CD3 bispecific antibody efficacy | Scientific reports | 31882897 | Healthy donor peripheral blood mononuclear cells (PBMCs) were purchased from ReachBio Research Labs. T cells were isolated by negative selection using the Dynabeads Save;Clec12axCD3 bsAbs were isolated by selective protein A affinity chromatography | Full Paper |
2019 | Ostrakhovitch, EA;Akakura, S;Sanokawa-Akakura, R;Tabibzadeh, S; | 3-Mercaptopyruvate sulfurtransferase disruption in dermal fibroblasts facilitates adipogenic trans-differentiation | Experimental cell research | 31634482 | Human dermal fibroblasts (HDF) were obtained from ATCC (Manassas, VA). Mesenchymal stem cells (MSCs), isolated from a 25-year-old male donor, were purchased from ReachBio Research lab (Seattle, WA). Chemicals were from Sigma-Aldrich Company (St Louis | Full Paper |
2019 | Radtke, S;Chan, YY;Sippel, TR;Kiem, HP;Rongvaux, A; | MISTRG mice support engraftment and assessment of nonhuman primate hematopoietic stem and progenitor cells | Experimental hematology | 30590092 | For CFC assays, 1000-1200 sort-purified NHP or CD34-subpopulations were seeded into 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA). Hematopoietic colonies were scored after 12-14 days. Arising colonies were identified as colony-forming unit- (CFU-) gr | Full Paper |
2019 | Radtke, S;Perez, AM;Venkataraman, R;Reddy, S;Haworth, KG;Humbert, O;Kiem, HP;Peterson, CW; | Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells | Journal of visualized experiments : JoVE | 30829324 | Protamine sulfate Sigma P-4505 14 mL Polypropylene Round-Bottom Tube Corning 352059 Colony Gel 1402 ReachBio 1402 QuadroMACS Separators Miltenyi Biotec 130-090-976 | Full Paper |
2018 | Cristescu, R;Mogg, R;Ayers, M;Albright, A;Murphy, E;Yearley, J;Sher, X;Liu, XQ;Lu, H;Nebozhyn, M;Zhang, C;Lunceford, JK;Joe, A;Cheng, J;Webber, AL;Ibrahim, N;Plimack, ER;Ott, PA;Seiwert, TY;Ribas, A;McClanahan, TK;Tomassini, JE;Loboda, A;Kaufman, D; | Pan-tumor genomic biomarkers for PD-1 checkpoint blockade-based immunotherapy | Science | 30309915 | Associations of TMB and the T cell–inflamed GEP with BOR and PFS were evaluated by using tumor samples from subgroups of patients treated with pembrolizumab in clinical trials who had WES data available. These included a discovery cohort of patients | Full Paper |
2018 | Smith, S;Lynch, R;Till, B;Cowan, A;Wu, Q;Voutsinas, J;Shadman, M;Blue, K;Shustov, A;Kanan, S;Low, L;Cassaday, R;Fromm, J;Gopal, A; | Pembrolizumab in Combination with Standard RCHOP Therapy for Previously Untreated Diffuse Large B-Cell Lymphoma | Blood | Secondary endpoints included response rates, overall (OS) and progression-free survival (PFS). Baseline PDL1 expression was analyzed centrally (Merck/Qualtek). Peripheral blood flow cytometry for changes in PD1, PDL-1, and PDL-2 subsets were performe | Full Paper | |
2018 | Doi, T;Piha-Paul, SA;Jalal, SI;Saraf, S;Lunceford, J;Koshiji, M;Bennouna, J; | Safety and Antitumor Activity of the Anti-Programmed Death-1 Antibody Pembrolizumab in Patients With Advanced Esophageal Carcinoma | J. Clin. Oncol. | 29116900 | PD-L1 Expression Analysis PD-L1 expression was assessed at screening by a central laboratory on the basis of a laboratory-developed prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA) using either an archived (formalin-fi | Full Paper |
2018 | Kanjanapan, Y;Lien, S;Yang, C;Clouthier, D;Elston, S;Jiang, H;Butler, M;Hogg, D;Ohashi, P;Pugh, T;Siu, L;Spreafico, A; | Genomic and immune landscape of metastatic melanoma (MM) treated with pembrolizumab (PEM). | JCO | Baseline (BL) and on treatment (post 2-3 doses of PEM) fresh tumor biopsies were collected for DNA/RNA sequencing, immune-profiling, and tumor PD-L1 expression by immunohistochemistry (QualTek), with longitudinal blood collection for immunophenotypi | Full Paper | |
2018 | Graff, J;Alumkal, J;Thompson, R;Moran, A;Thomas, G;Wood, M;Drake, C;Slottke, R;Beer, T; | Pembrolizumab (Pembro) plus enzalutamide (Enz) in metastatic castration resistant prostate cancer (mCRPC): Extended follow up. | JCO | The other 2 had neither. Of the NR who had sequencing, 4 had a DNA repair defects, and none had MSI. None of the biopsies showed tumoral PD-L1 expression (Qualtek, 22C3). Conclusions: Pembro has activity in mCRPC when added to Enz | Full Paper | |
2018 | Kelly, C;Bowler, T;Munhoz, R;Chi, P;Dickson, M;Gounder, M;Keohan, M;Dholakia, R;Condy, M;Singer, S;Crago, A;Yoon, S;Ariyan, C;Hwang, S;Erinjeri, J;Antonescu, C;Qin, L;Tap, W;D'Angelo, S; | A phase II study of talimogene laherparepvec (T-VEC) and pembrolizumab in patients with metastatic sarcoma. | JCO | overall survival. Correlative studies included PD-L1 expression by IHC (Qualtek), multiplex IHC, characterization of tumor infiltrating lymphocytes, mutational burden/neoantigen analysis, and T cell receptor clonality. Results | Full Paper | |
2018 | Smith, JJ;Timoshevskaya, N;Ye, C;Holt, C;Keinath, MC;Parker, HJ;Cook, ME;Hess, JE;Narum, SR;Lamanna, F;Kaessmann, H;Timoshevskiy, VA;Waterbury, CKM;Saraceno, C;Wiedemann, LM;Robb, SMC;Baker, C;Eichler, EE;Hockman, D;Sauka-Spengler, T;Yandell, M;Krumlauf, R;Elgar, G;Amemiya, CT; | The sea lamprey germline genome provides insights into programmed genome rearrangement and vertebrate evolution | Nat. Genet. | 29358652 | Sequencing. Fragment libraries were prepared by Covaris shearing of sperm genomic DNA isolated from a single individual and size selected to achieve average insert sizes of ~205 and 231bp. These libraries were sequenced on the Illumina HiSeq2000 plat | Full Paper |
2018 | D'Angelo, SP;Melchiori, L;Merchant, MS;Bernstein, D;Glod, J;Kaplan, R;Grupp, S;Tap, WD;Chagin, K;Binder, GK;Basu, S;Lowther, DE;Wang, R;Bath, N;Tipping, A;Betts, G;Ramachandran, I;Navenot, JM;Zhang, H;Wells, DK;Van Winkle, E;Kari, G;Trivedi, T;Holdich, T;Pandite, L;Amado, R;Mackall, CL; | Antitumor Activity Associated with Prolonged Persistence of Adoptively Transferred NY-ESO-1 c259T Cells in Synovial Sarcoma | Cancer Discov | 29891538 | Serial 5-μm sections of formalin-fixed, paraffin-embedded tissues were analyzed for the following single markers by IHC: NY-ESO-1, Pan-CK (Clone AE1/AE3/PCK26, Ventana), CD45 (Clone 2B11 + PD7/26, Ventana), CD3 (Clone 2GV6, Ventana), CD8 (Clone C8/14 | Full Paper |
2018 | Dejanovic, B;Huntley, MA;De Mazière, A;Meilandt, WJ;Wu, T;Srinivasan, K;Jiang, Z;Gandham, V;Friedman, BA;Ngu, H;Foreman, O;Carano, RAD;Chih, B;Klumperman, J;Bakalarski, C;Hanson, JE;Sheng, M; | Changes in the Synaptic Proteome in Tauopathy and Rescue of Tau-Induced Synapse Loss by C1q Antibodies | Neuron | 30392797 | Biological Samples Human brain tissue Folio Biosciences N/A | Full Paper |
2018 | Schafer, PH;Ye, Y;Wu, L;Kosek, J;Ringheim, G;Yang, Z;Liu, L;Thomas, M;Palmisano, M;Chopra, R; | Cereblon modulator iberdomide induces degradation of the transcription factors Ikaros and Aiolos: immunomodulation in healthy volunteers and relevance to systemic lupus erythematosus | Ann. Rheum. Dis. | 29945920 | study in healthy volunteers. Methods. Gene expression studies. Viably frozen PBMCs from 11 patients with SLE and 10 healthy volunteers were obtained from Conversant Bio (Huntsville, Alabama, USA). Informed consent was | Full Paper |
2018 | Liles, JT;Corkey, BK;Notte, GT;Budas, GR;Lansdon, EB;Hinojosa-Kirschenbaum, F;Badal, SS;Lee, M;Schultz, BE;Wise, S;Pendem, S;Graupe, M;Castonguay, L;Koch, KA;Wong, MH;Papalia, GA;French, DM;Sullivan, T;Huntzicker, EG;Ma, FY;Nikolic-Paterson, DJ;Altuhaifi, T;Yang, H;Fogo, AB;Breckenridge, DG; | ASK1 contributes to fibrosis and dysfunction in models of kidney disease | J. Clin. Invest. | 30024858 | ... NAL BIOPSIES AND DB/DB ENOS–/– MOUSE KIDNEYS Formalin-fixed paraffin-embedded renal biopsies from 10 patients with DKD and from 7 donors without kidney disease (“healthy”) were obtained from Folio Biosciences. Kidney sections were prepared from | Full Paper |
2018 | Gadgeel, SM;Pennell, NA;Fidler, MJ;Halmos, B;Bonomi, P;Stevenson, J;Schneider, B;Sukari, A;Ventimiglia, J;Chen, W;Galasso, C;Wozniak, A;Boerner, J;Kalemkerian, GP; | Phase II Study of Maintenance Pembrolizumab in Patients with Extensive-Stage Small Cell Lung Cancer (SCLC) | J Thorac Oncol | 29775808 | than every four cycles. Retrieval of pretreatment biopsy specimens for assessment of PD-L1 expression in tumor cells and stromal tissue was conducted by Qualtek Clinical Laboratories (Newton, PA). Assessment of tumor PD | Full Paper |
2018 | Roberts, BS;Hardigan, AA;Moore, DE;Ramaker, RC;Jones, AL;Fitz-Gerald, MB;Cooper, GM;Wilcox, CM;Kimberly, RP;Myers, RM; | Discovery and Validation of Circulating Biomarkers of Colorectal Adenoma by High-Depth Small RNA Sequencing | Clin. Cancer Res. | 29490987 | HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama. | Full Paper |
2018 | Tucker, DW;Getchell, CR;McCarthy, ET;Ohman, AW;Sasamoto, N;Xu, S;Ko, JY;Gupta, M;Shafrir, A;Medina, JE;Lee, JJ;MacDonald, LA;Malik, A;Hasselblatt, KT;Li, W;Zhang, H;Kaplan, SJ;Murphy, GF;Hirsch, MS;Liu, JF;Matulonis, UA;Terry, KL;Lian, CG;Dinulescu, DM; | Epigenetic Reprogramming Strategies to Reverse Global Loss of 5-Hydroxymethylcytosine, a Prognostic Factor for Poor Survival in High-grade Serous Ovarian Cancer | Clin. Cancer Res. | 29263182 | ... s of 5-hmC levels in a large biorepository of HGSOC cases by using a patient tumor microarray (TMA) from the Dana-Farber Cancer Institute (DFCI, Boston, MA; ref. 22) and a commercially available TMA (Folio Biosciences). We observed strong 5-hmC s | Full Paper |
2018 | Subbiah, V;Murthy, R;Hong, DS;Prins, RM;Hosing, C;Hendricks, K;Kolli, D;Noffsinger, L;Brown, R;McGuire, M;Fu, S;Piha-Paul, S;Naing, A;Conley, AP;Benjamin, RS;Kaur, I;Bosch, ML; | Cytokines Produced by Dendritic Cells Administered Intratumorally Correlate with Clinical Outcome in Patients with Diverse Cancers | Clin. Cancer Res. | 30018119 | Biopsied tumors were formalin fixed and paraffin embedded (FFPE) using standard methods. All immunohistochemistry was performed by QualTek Molecular Laboratories (Santa Barbara, CA). In situ detection of IFNγ and TNFα transcripts in FFPE specimens w | Full Paper |
2018 | Joseph, RW;Elassaiss-Schaap, J;Kefford, R;Hwu, WJ;Wolchok, JD;Joshua, AM;Ribas, A;Hodi, FS;Hamid, O;Robert, C;Daud, A;Dronca, R;Hersey, P;Weber, JS;Patnaik, A;de Alwis, DP;Perrone, A;Zhang, J;Kang, SP;Ebbinghaus, S;Anderson, KM;Gangadhar, TC; | Baseline Tumor Size Is an Independent Prognostic Factor for Overall Survival in Patients with Melanoma Treated with Pembrolizumab | Clin. Cancer Res. | 29685882 | Tumor PD-L1 expression was assessed by a prototype IHC assay (QualTek Molecular Laboratories; ref. 14) in pretreatment tumor biopsy samples using the 22C3 antibody (Merck & Co., Inc.). PD-L1 positivity was defined as membranous staining in 1% of tumo | Full Paper |
2018 | Rugo, HS;Delord, JP;Im, SA;Ott, PA;Piha-Paul, SA;Bedard, PL;Sachdev, J;Le Tourneau, C;van Brummelen, EMJ;Varga, A;Salgado, R;Loi, S;Saraf, S;Pietrangelo, D;Karantza, V;Tan, AR; | Safety and Antitumor Activity of Pembrolizumab in Patients with Estrogen Receptor-Positive/Human Epidermal Growth Factor Receptor 2-Negative Advanced Breast Cancer | Clin. Cancer Res. | 29559561 | An archival or newly obtained core or excisional biopsy specimen from a nonirradiated tumor lesion was collected from each patient for assessment of PD-L1 expression. Tumor PD-L1 expression was assessed by immunohistochemistry at a central laboratory | Full Paper |
2018 | Thompson, ML;Finnila, CR;Bowling, KM;Brothers, KB;Neu, MB;Amaral, MD;Hiatt, SM;East, KM;Gray, DE;Lawlor, JMJ;Kelley, WV;Lose, EJ;Rich, CA;Simmons, S;Levy, SE;Myers, RM;Barsh, GS;Bebin, EM;Cooper, GM; | Genomic sequencing identifies secondary findings in a cohort of parent study participants | Genet. Med. | 29790872 | to change their preferences at the return of results appointment. Whole exome and genome sequencing Blood samples were sent for sequencing at the HudsonAlpha Genomic Services Laboratory (http://gsl.hudsonalpha.org). Genomic DNA was isolated from peri | Full Paper |
2018 | Pena, LDM;Jiang, YH;Schoch, K;Spillmann, RC;Walley, N;Stong, N;Rapisardo Horn, S;Sullivan, JA;McConkie-Rosell, A;Kansagra, S;Smith, EC;El-Dairi, M;Bellet, J;Keels, MA;Jasien, J;Kranz, PG;Noel, R;Nagaraj, SK;Lark, RK;Wechsler, DSG;Del Gaudio, D;Leung, ML;Hendon, LG;Parker, CC;Jones, KL;, ;Goldstein, DB;Shashi, V; | Looking beyond the exome: a phenotype-first approach to molecular diagnostic resolution in rare and undiagnosed diseases | Genet. Med. | 28914269 | Kimberly Splinter Harvard University (CC) David P. Bick HudsonAlpha Camille L. Birch HudsonAlpha Braden E. Boone HudsonAlpha Donna M. Brown HudsonAlpha Daniel C. Dorset HudsonAlpha Lori H. Handley HudsonAlpha Howard J. Jacob HudsonAlpha Angela L. Jo | Full Paper |
2018 | Abraham, B;Pharma, GUCB;Sankyo, HD; | Disclosure of relevant financial relationships | Journal of the American Academy of Dermatology | Abramovits, William; Grants - AbbVie, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant Bio, Dermavant Abramovits, William; Honoraria - AbbVie, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant Bio, D | Full Paper | |
2018 | Menard, LC;Fischer, P;Kakrecha, B;Linsley, PS;Wambre, E;Liu, MC;Rust, BJ;Lee, D;Penhallow, B;Manjarrez Orduno, N;Nadler, SG; | Renal Cell Carcinoma (RCC) Tumors Display Large Expansion of Double Positive (DP) CD4+CD8+ T Cells With Expression of Exhaustion Markers | Front Immunol | 30534127 | Samples for the discovery and replication cohorts were provided by Folio-Conversant Bio (AL), MT Group (CA), Rutgers-CINJ or the Benaroya Research Institute. All patients gave written informed consent at time of sample collection according to IRB pro | Full Paper |
2018 | Tisdale, J;Kanter, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Schmidt, M;Miller, A;Pierciey, F;Shi, W;Ribeil, J;Asmal, M;Thompson, A;Walters, M; | Current Results of Lentiglobin Gene Therapy in Patients with Severe Sickle Cell Disease Treated Under a Refined Protocol in the Phase 1 Hgb-206 Study | Blood | Background β-globin gene transfer has the potential for substantial clinical benefit in patients with sickle cell disease (SCD). LentiGlobin Drug Product (DP) contains autologous CD34+ hematopoietic stem cells (HSCs) transduced with th | Full Paper | |
2018 | Klemann, CJHM;Xicoy, H;Poelmans, G;Bloem, BR;Martens, GJM;Visser, JE; | Physical Exercise Modulates L-DOPA-Regulated Molecular Pathways in the MPTP Mouse Model of Parkinson's Disease | Mol. Neurobiol. | 29019056 | pooled for RNAseq analysis. RNA Sequencing and Data Processing. All RNA samples were subjected to RNA sequencing (RNAseq; HudsonAlpha Genomic Services Lab, Huntsville, AL) as performed before [37]. In short, total | Full Paper |
2018 | Van Laar, R;Lincoln, M;Van Laar, B; | Development and validation of a plasma-based melanoma biomarker suitable for clinical use | Br. J. Cancer | 29360813 | expression profiles of individuals with and without melanoma, 0.5-2 ml of cell-free plasma was obtained from 16 healthy controls and 32 Caucasian individuals at the time of their diagnosis with cutaneous melanoma (Cureline Inc., CA, USA and Folio Bio | Full Paper |
2018 | Buatois, V;Johnson, Z;Salgado-Pires, S;Papaioannou, A;Hatterer, E;Chauchet, X;Richard, F;Barba, L;Daubeuf, B;Cons, L;Broyer, L;D'Asaro, M;Matthes, T;LeGallou, S;Fest, T;Tarte, K;Clarke Hinojosa, RK;Genescà Ferrer, E;Ribera, JM;Dey, A;Bailey, K;Fielding, AK;Eissenberg, L;Ritchey, J;Rettig, M;DiPersio, JF;Kosco-Vilbois, MH;Masternak, K;Fischer, N;Shang, L;Ferlin, WG; | Preclinical Development of a Bispecific Antibody that Safely and Effectively Targets CD19 and CD47 for the Treatment of B-Cell Lymphoma and Leukemia | Mol. Cancer Ther. | 29743205 | Cells were cultured at 37°C and 5% CO2. Primary samples were collected from Conversant Biologics (Huntsville, USA), the Leukemia Research Center (Glasgow, UK), the Geneva University Hospital (Geneva, Switzerland) ;... tometry and finally selecte | Full Paper |
2018 | Foulk, B;Schaffer, M;Gross, S;Rao, C;Smirnov, D;Connelly, MC;Chaturvedi, S;Reddy, M;Brittingham, G;Mata, M;Repollet, M;Rojas, C;Auclair, D;DeRome, M;, ;Weiss, B;Sasser, AK; | Enumeration and characterization of circulating multiple myeloma cells in patients with plasma cell disorders | Br. J. Haematol. | 29105742 | Verification cohort samples were obtained from Conversant Biologics (Huntsville, AL, USA) under a biospecimen purchase agreement. These samples represent a heterogenous cohort of patients with active disease and those in remission | Full Paper |
2018 | Pekcec, A;Schülert, N;Stierstorfer, B;Deiana, S;Dorner-Ciossek, C;Rosenbrock, H; | Targeting the dopamine D1 receptor or its downstream signalling by inhibiting phosphodiesterase-1 improves cognitive performance | Br. J. Pharmacol. | 29726015 | paraffin blocks. Human brain tissue from healthy donors (Folio Biosciences, Ohio, USA) was fixed in 4% paraformaldehyde, and samples containing PFC were identified, trimmed and processed into paraffin blocks. All procurement | Full Paper |
2018 | Maity, A;Mick, R;Huang, AC;George, SM;Farwell, MD;Lukens, JN;Berman, AT;Mitchell, TC;Bauml, J;Schuchter, LM;O'Hara, M;Lin, LL;Demichele, A;Christodouleas, JP;Haas, NB;Patsch, DM;Hahn, SM;Minn, AJ;Wherry, EJ;Vonderheide, RH; | A phase I trial of pembrolizumab with hypofractionated radiotherapy in patients with metastatic solid tumours | Br. J. Cancer | 30318516 | ange in volume of the irradiated (index) lesion was also calculated, but this was not used in the RECIST measurement. Immunohistochemical staining PD-L1 staining was performed by an outside company (QualTek Molecular Laboratories, Newtown, PA, USA) | Full Paper |
2018 | Mehra, R;Seiwert, TY;Gupta, S;Weiss, J;Gluck, I;Eder, JP;Burtness, B;Tahara, M;Keam, B;Kang, H;Muro, K;Geva, R;Chung, HC;Lin, CC;Aurora-Garg, D;Ray, A;Pathiraja, K;Cheng, J;Chow, LQM;Haddad, R; | Efficacy and safety of pembrolizumab in recurrent/metastatic head and neck squamous cell carcinoma: pooled analyses after long-term follow-up in KEYNOTE-012 | Br. J. Cancer | 29955135 | PD-L1 expression status during screening was determined using a prototype PD-L1 immunohistochemical assay 18 performed at a laboratory site (QualTek) accredited by the College of American Pathologists and Clinical Laboratory Improvement Amendments, a | Full Paper |
2018 | Wein, L;Luen, SJ;Savas, P;Salgado, R;Loi, S; | Checkpoint blockade in the treatment of breast cancer: current status and future directions | Br. J. Cancer | 29808015 | Patients were required to be PD-L1 positive by immunohistochemistry (IHC), which was defined as PD-L1 positivity in the stroma or ≥1% tumour cells using their Qualtek assay.28 Most patients were heavily pre-treated; the median number of previous line | Full Paper |
2018 | Kanter, J;Tisdale, J;Kwiatkowski, J;Krishnamurti, L;Mapara, M;Schmidt, M;Miller, A;Pierciey, F;Shi, W;Ribeil, J;Walters, M;Thompson, A; | Outcomes for Initial Patient Cohorts with up to 33 Months of Follow-up in the Hgb-206 Phase 1 Trial | Blood | Background Sickle cell disease (SCD) is a progressively debilitating genetic disease causing significant morbidity and early mortality for which a universal curative therapy is lacking. Expression of an anti-sickling β-globin via gene | Full Paper | |
2018 | Bosco, EE;Christie, RJ;Carrasco, R;Sabol, D;Zha, J;DaCosta, K;Brown, L;Kennedy, M;Meekin, J;Phipps, S;Ayriss, J;Du, Q;Bezabeh, B;Chowdhury, P;Breen, S;Chen, C;Reed, M;Hinrichs, M;Zhong, H;Xiao, Z;Dixit, R;Herbst, R;Tice, DA; | Preclinical evaluation of a GFRA1 targeted antibody-drug conjugate in breast cancer | Oncotarget | 29796165 | ... Fisher Scientific, Waltham, MA, USA) were coated with 5 mg/ml 9B3 antibody at 4○C overnight, washed, and blocked with 1% bovine serum albumin (Sigma-Aldrich, St Louis, MO). Human serum samples from Conversant Bio (Huntsville, AL) or from cell-lin | Full Paper |
2018 | Xu, Z;Wang, S;Li, Y;Zhu, F;Huang, J; | PRIM: An Efficient Preconditioning Iterative Reweighted Least Squares Method for Parallel Brain MRI Reconstruction | Neuroinformatics | 29423650 | International Holdings Inc. Albin, Mary, Westinghouse Alexander, William, Qualtek Manufacturing, Inc. Alexander, Chuck, Solid Concepts, Inc. Alexopoulos, Nick, Broadcom Corporation Alford, Charles, UES, Inc. Alvarado, Igor | Full Paper |
2018 | Locatelli, F;Walters, M;Kwiatkowski, J;Porter, J;Sauer, M;Thuret, I;Hongeng, S;Kulozik, A;Lal, A;Thrasher, A;Yannaki, E;Elliot, H;Tao, G;Asmal, M;Thompson, A; | Lentiglobin Gene Therapy for Patients with Transfusion-Dependent β-Thalassemia (TDT): Results from the Phase 3 Northstar-2 and Northstar-3 Studies | Blood | Background Transfusion-dependent β-thalassemia (TDT) is a severe genetic disease caused by impaired β-globin production, leading to severe anemia, lifelong transfusion dependence with iron overload and serious comorbidities. Gene thera | Full Paper | |
2018 | Li, C;Mathews, V;George, B;Kim, S;Hebert, K;Jiang, H;Li, C;Zhu, Y;Keesler, D;Agarwal, R;Boelens, J;Dvorak, C;Auletta, J;Goyal, R;Hanna, R;Kasow, K;Margolis, D;Shenoy, S;Walters, M;Eapen, M; | Related and Unrelated Donor Transplantation for β Thalassemia Major: Results of an International Survey | Blood | We studied 1110 children, adolescents and young adults with β thalassemia major transplanted with grafts from HLA-matched relative (n=677; 61%), HLA-mismatched relative (n=78; 7%), HLA-matched unrelated (n=252; 23%) and HLA-mismatched unrelated (n=10 | Full Paper | |
2018 | Rasko, J;Thompson, A;Kwiatkowski, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;Schmidt, M;Kletzel, M;Leboulch, P;Vichinsky, E;Deary, B;Chen, Y;Asmal, M;Walters, M; | Clinical Outcomes of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia Following Completion of the Northstar HGB-204 Study | Blood | Background Advances in red blood cell (RBC) transfusion and chelation have improved the prognosis of patients with transfusion-dependent β-thalassemia (TDT); however, many patients experience organ damage due to iron overload and other | Full Paper | |
2018 | Godwin, RL;Opatova, V;Garrison, NL;Hamilton, CA;Bond, JE; | Phylogeny of a cosmopolitan family of morphologically conserved trapdoor spiders (Mygalomorphae, Ctenizidae) using Anchored Hybrid Enrichment, with a description of the family, Halonoproctidae Pocock 1901 | Mol. Phylogenet. Evol. | 29656103 | Phylogeny of a cosmopolitan family of morphologically conserved trapdoor spiders (Mygalomorphae, Ctenizidae) using Anchored Hybrid Enrichment, with a description of the family, Halonoproctidae Pocock 1901 | Full Paper |
2018 | Hansen, R;Firdaus, SJ;Li, S;Janes, MR;Zhang, J;Liu, Y;Zarrinkar, PP; | An Internally Controlled Quantitative Target Occupancy Assay for Covalent Inhibitors | Sci Rep | 30254226 | spectrometry. Clinical specimens. Clinical FFPE specimens were purchased from Conversant Bio (Huntsville, AL) and Asterand Bioscience (Detroit, MI). Conversant and Asterand comply with all applicable regulations. Animal studies | Full Paper |
2018 | Potts, AH;Leng, Y;Babitzke, P;Romeo, T; | Examination of Csr regulatory circuitry using epistasis analysis with RNA-seq (Epi-seq) confirms that CsrD affects gene expression via CsrA, CsrB and CsrC | Sci Rep | 29599472 | RNA-seq libraries were then generated with the Stranded RNA-Seq Library Preparation Kit for Illumina (KAPA) and NEBNext Multiplex Oligos for Illumina adaptors (NEB) according to the manufacturer’s instructions for 100 ng of starting material and a me | Full Paper |
2018 | Friedman, KM;Garrett, TE;Evans, JW;Horton, HM;Latimer, HJ;Seidel, SL;Horvath, CJ;Morgan, RA; | Effective Targeting of Multiple B-Cell Maturation Antigen-Expressing Hematological Malignances by Anti-B-Cell Maturation Antigen Chimeric Antigen Receptor T Cells | Hum. Gene Ther. | 29641319 | LLC. Peripheral blood mononuclear cells (PBMCs) were isolated and cryopreserved in the authors' laboratory. Whole blood from two CLL patients was obtained from Conversant Biologics, Inc. Immunohistochemistry. Twenty | Full Paper |
2018 | Vasanthavel, S;Meenakshi, K;Nivedha, V;Ballamurugan, AM;Kannan, S; | Tuning the structural and mechanical properties in ZrO2-SiO2 binary system through Y3+ inclusions | Mater Sci Eng C Mater Biol Appl | 29519433 | Zirconia (ZrO 2 ) and Alumina (Al 2 O 3 ) are conversant bio-inert ceramics known for their superior mechanical properties while hydroxyapatite [Ca 5 (PO 4 ) 3 OH, HAP], β-tricalciumphosphate [β-Ca 3 (PO 4 ) 2 , β-TCP] and SiO 2 based bioactive glass | Full Paper |
2018 | Schlegel, C;Lapierre, LA;Weis, VG;Williams, JA;Kaji, I;Pinzon-Guzman, C;Prasad, N;Boone, B;Jones, A;Correa, H;Levy, SE;Han, X;Wang, M;Thomsen, K;Acra, S;Goldenring, JR; | Reversible deficits in apical transporter trafficking associated with deficiency in diacylglycerol acyltransferase | Traffic | 30095213 | Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee.++Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee.++Department of Surgery, Vanderbilt University School of Medicine, Nashville | Full Paper |
2018 | Selvan, N;George, S;Serajee, FJ;Shaw, M;Hobson, L;Kalscheuer, V;Prasad, N;Levy, SE;Taylor, J;Aftimos, S;Schwartz, CE;Huq, AM;Gecz, J;Wells, L; | O-GlcNAc transferase missense mutations linked to X-linked intellectual disability deregulate genes involved in cell fate determination and signaling | J. Biol. Chem. | 29769320 | From the Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602.++From the Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, Universit | Full Paper |
2018 | Edwardson, CF;Hollibaugh, JT; | Composition and Activity of Microbial Communities along the Redox Gradient of an Alkaline, Hypersaline, Lake | Front Microbiol | 29445359 | Samples were pooled and sequenced on one lane of an Illumina HiSeq2500 in Rapid Run mode with the 150PE (300 cycle) kit at HudsonAlpha Genomic Services Lab (Huntsville, AL). Bioinformatics of 16S rRNA Sequences | Full Paper |
2018 | Xu, J;Su, X;Bonizzoni, M;Zhong, D;Li, Y;Zhou, G;Nguyen, H;Tong, S;Yan, G;Chen, XG; | Comparative transcriptome analysis and RNA interference reveal CYP6A8 and SNPs related to pyrethroid resistance in Aedes albopictus | PLoS Negl Trop Dis | 30418967 | ; RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). A total of 24 cDNA libraries were prepared individually from 12 susceptible and 12 resistant mosqu | Full Paper |
2018 | Biase, FH;Kimble, KM; | Functional signaling and gene regulatory networks between the oocyte and the surrounding cumulus cells | BMC Genomics | 29747587 | The samples were subjected to 16 cycles of PCR amplification. For all samples, we used 1 ng of amplified cDNA for library preparation with the Nextera XT DNA library preg Kit (Illumina, Inc.), as per the procedures described in the SMART-seq2 protoco | Full Paper |
2018 | Dickinson, SE;Griffin, BA;Elmore, MF;Kriese-Anderson, L;Elmore, JB;Dyce, PW;Rodning, SP;Biase, FH; | Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potential | BMC Genomics | 29426285 | Libraries were prepared with the TruSeq Stranded mRNA Library Prep kit (Illumina, Inc., San Diego, CA) following manufacturer’s instructions. Libraries were quantified with Qubit™ dsDNA High Sensitivity Assay Kit (Eurogene, OR) and quality was evalua | Full Paper |
2018 | Lin, EM;Gong, J;Klempner, SJ;Chao, J; | Advances in immuno-oncology biomarkers for gastroesophageal cancer: Programmed death ligand 1, microsatellite instability, and beyond | World J. Gastroenterol. | 29991874 | Presence of tumor PD-L1 expression was a requirement for enrollment, with 40% of the patients screened demonstrating PD-L1-positive tumors. PD-L1 expression was detected using the 22C3 antibody with a prototype assay using QualTek or Dako platforms | Full Paper |
2018 | Fan, J;Lerner, J;Wyatt, MK;Cai, P;Peterson, K;Dong, L;Wistow, G; | The klotho-related protein KLPH (lctl) has preferred expression in lens and is essential for expression of clic5 and normal lens suture formation | Exp. Eye Res. | 29425878 | XAR film at −70 °C. 2.3. In situ hybridization (ISH). Frozen tissues were cut into 10 μm sections, mounted on gelatin-coated slides and stored at −80 °C. ISH was performed at Phylogeny Inc. (Columbus, OH). Slides were fixed in | Full Paper |
2018 | Hiatt, SM;Amaral, MD;Bowling, KM;Finnila, CR;Thompson, ML;Gray, DE;Lawlor, JMJ;Cochran, JN;Bebin, EM;Brothers, KB;East, KM;Kelley, WV;Lamb, NE;Levy, SE;Lose, EJ;Neu, MB;Rich, CA;Simmons, S;Myers, RM;Barsh, GS;Cooper, GM; | Systematic reanalysis of genomic data improves quality of variant interpretation | Clin. Genet. | 29652076 | HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama. | Full Paper |
2018 | Håvik, AL;Bruland, O;Aarhus, M;Kalland, KH;Stokowy, T;Lund-Johansen, M;Knappskog, PM; | Screening for viral nucleic acids in vestibular schwannoma | J. Neurovirol. | 30168016 | ; RNA sequencing on RNA extracted from five sVS tissue biopsies was performed by HudsonAlpha (HudsonAlpha Genomic Services Laboratory, 601 Genome Way, Huntsville, AL 35806 USA) using their "Poly A RNA-sequencing" service including 50 cycles of paire | Full Paper |
2018 | Mohan, S;Greenstein, I;Ng, C;Frazier, K;Nguyen, G;Harding, L;Barlow, D; | Assessing N w-hydroxy-L-arginine applicability as a novel ethnic specific estrogen-negative breast cancer marker | Amino Acids | 29260322 | Collection (Manassas, VA). We received de-identified plasma samples from Cooperative Human Tissue Network (Philadelphia, PA), Tissue-for-Research (Atlanta, GA), and Conversant Bio (Huntsville, AL). The de-identified | Full Paper |
2018 | Zhu, J;Gan, X;Ao, Q;Shen, X;Tan, Y;Chen, M;Luo, Y;Wang, H;Jiang, H;Li, C; | Basal polarization of the immune responses to Streptococcus agalactiae susceptible and resistant tilapia (Oreochromis niloticus) | Fish Shellfish Immunol. | 29454032 | ; For each timepoint, equal amounts of RNA from the three replicates were pooled for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) | Full Paper |
2018 | Porter, ET;Porter, FS; | A Strain Gauge Monitor (SGM) for Continuous Valve Gape Measurements in Bivalve Molluscs in Response to Laboratory Induced Diel-cycling Hypoxia and pH | J Vis Exp | 30124658 | Name Company Catalog Number Comments Campbell CR 10x data logger Campbell Scientific, Logan, Utah Or other data logger. At Campbell the CR 10X has been replaced with the CR 1000 Campbell CR 10x multiplexer Campbell Scientific, Logan, Utah Data logg | Full Paper |
2018 | Yang, Y;Fu, Q;Liu, Y;Wang, X;Dunham, R;Liu, S;Bao, L;Zeng, Q;Zhou, T;Li, N;Qin, Z;Jiang, C;Gao, D;Liu, Z; | Comparative transcriptome analysis reveals conserved branching morphogenesis related genes involved in chamber formation of catfish swimbladder | Physiol. Genomics | 29167198 | Equal amounts of RNA from the three replicates for each sample were pooled together for RNA-Seq library preparation. The remaining replicate pools were preserved as biological replicates for quantitative (q)RT-PCR validation. The cDNA libraries were | Full Paper |
2018 | Cohen, RB;Delord, JP;Doi, T;Piha-Paul, SA;Liu, SV;Gilbert, J;Algazi, AP;Damian, S;Hong, RL;Le Tourneau, C;Day, D;Varga, A;Elez, E;Wallmark, J;Saraf, S;Thanigaimani, P;Cheng, J;Keam, B; | Pembrolizumab for the Treatment of Advanced Salivary Gland Carcinoma: Findings of the Phase 1b KEYNOTE-028 Study | Am. J. Clin. Oncol. | 29462123 | Tumor PD-L1 positivity was determined at a central laboratory using formalin-fixed archived or newly obtained biopsy samples and a prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA),17 based on the 22C3 antibody (Merck & | Full Paper |
2018 | Glover, ME;McCoy, CR;Shupe, EA;Unroe, KA;Jackson, NL;Clinton, SM; | Perinatal exposure to the SSRI paroxetine alters the methylome landscape of the developing dentate gyrus | Eur. J. Neurosci. | 30585666 | ; quantified (Quant-iT high sensitivity DNA Assay Kit, Invitrogen, Grand Island, NY). Next- generation sequencing was performed at HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) using NEBNext reagents (New Engla | Full Paper |
2018 | Verwimp, C;Ruttink, T;Muylle, H;Van Glabeke, S;Cnops, G;Quataert, P;Honnay, O;Roldán-Ruiz, I; | Temporal changes in genetic diversity and forage yield of perennial ryegrass in monoculture and in combination with red clover in swards | PLoS ONE | 30408053 | The protocol for the 112 tissue pool samples was slightly adjusted, based on the results of the validation step. In this case, the 50 μL bead-purified ligate was split into three aliquots, and 16.7 μL was used for three replicate PCR reactions to acc | Full Paper |
2018 | Geister, KA;Timms, AE;Beier, DR; | Optimizing Genomic Methods for Mapping and Identification of Candidate Variants in ENU Mutagenesis Screens Using Inbred Mice | G3 (Bethesda) | 29208648 | ... centrifugation. DNA was tested at SCRI for purity and quality using a Nanodrop spectrophotometer and the Qubit dsDNA Broad Range Assay (#Q32850; Thermo Fisher Scientific). DNA samples were shipped to HudsonAlpha Genomic Services Laboratory (Hunts | Full Paper |
2018 | Papanek, B;O'Dell, KB;Manga, P;Giannone, RJ;Klingeman, DM;Hettich, RL;Brown, SD;Guss, AM; | Transcriptomic and proteomic changes from medium supplementation and strain evolution in high-yielding Clostridium thermocellum strains | J. Ind. Microbiol. Biotechnol. | 30187243 | ; Sequencing was completed using a SR50 sequencing protocol on an Illumina HiSeq 2500 platform (HudsonAlpha Genomic Services Laboratory; Huntsville, AL, USA). RNA-Seq analysis. The analysis of the raw RNA-Seq data was performed as previously describ | Full Paper |
2018 | Ziai, J;Gilbert, HN;Foreman, O;Eastham-Anderson, J;Chu, F;Huseni, M;Kim, JM; | CD8+ T cell infiltration in breast and colon cancer: A histologic and statistical analysis | PLoS ONE | 29320521 | IV were represented. Colorectal cancer samples were procured from Conversant Bio and included moderately differentiated adenocarcinoma (9), mucinous carcinoma (1) and rectal (2) carcinoma cases. Samples contained | Full Paper |
2018 | Gallery, M;Zhang, J;Bradley, DP;Brauer, P;Cvet, D;Estevam, J;Danaee, H;Greenfield, E;Li, P;Manfredi, M;Loke, HK;Rabino, C;Stringer, B;Williamson, M;Wyant, T;Yang, J;Zhu, Q;Abu-Yousif, A;Veiby, OP; | A monomethyl auristatin E-conjugated antibody to guanylyl cyclase C is cytotoxic to target-expressing cells in vitro and in vivo | PLoS ONE | 29370189 | scanning system. Colon tissue microarrays (US Biomax) were stained and scored by two independent pathologists following a protocol developed at Qualtek Molecular Laboratories on their Tek-Mate automated stainer. Briefly ;ith hematoxylin and imag | Full Paper |
2018 | Lagatie, O;Verheyen, A;Nijs, E;Van Dorst, B;Batsa Debrah, L;Debrah, A;Supali, T;Sartono, E;Stuyver, LJ; | Evaluation of the Diagnostic Performance of Onchocerca volvulus Linear Epitopes in a Peptide Enzyme-Linked Immunosorbent Assay | Am. J. Trop. Med. Hyg. | 29313477 | (Glasgow, Scotland). A sec- ond healthy control sample set was composed of 49 plasma samples from healthy individuals from Belgium.20-24 Further- more, different sets of plasma samples were obtained from Discovery Life Sciences, Inc | Full Paper |
2018 | Andreotta, PW;Arold, S;Kenyon, J;Spicer, D;Woodman, P;Berry, E;Brogan, T;Kong, S;Okerholm, P;Russell, V;Clarke, RW;Hava, DL; | Inhaled calcium salts inhibit tobacco smoke-induced inflammation by modulating expression of chemokines and cytokines | Pulm Pharmacol Ther | 30359782 | Secreted protein expression and gene expression were assayed 2 h after LPS stimulation. 2.5. Human macrophage cultures. Human CD14 + monocytes were purchased from Conversant Biologics and differentiated into macrophages in culture | Full Paper |
2018 | Fall, L;Clevenger, J;McGregor, C; | Assay development and marker validation for marker assisted selection of Fusarium oxysporum f. sp. niveum race 1 in watermelon | Mol Breeding | for sequencing. Library construction and whole genome Illumina sequencing on an Illumina HiSeq X (Illumina, Inc., San Diego, CA) were performed at HudsonAlpha Genomic services laboratory (Huntsville, AL). Constructed | Full Paper | |
2018 | Zobel-Thropp, PA;Bulger, EA;Cordes, MHJ;Binford, GJ;Gillespie, RG;Brewer, MS; | Sexually dimorphic venom proteins in long-jawed orb-weaving spiders (Tetragnatha) comprise novel gene families | PeerJ | 29876146 | RNA extractions from the BF population of T. versicolor males (two) and females (10) were shipped to the Genomic Services Lab at HudsonAlpha (Huntsville, AL, USA), where cDNA libraries were prepared from total RNA (poly-A isolation, Illumina TruSeq R | Full Paper |
2018 | Awad, H;Bratasz, A;Nuovo, G;Burry, R;Meng, X;Kelani, H;Brown, M;Ramadan, ME;Williams, J;Bouhliqah, L;Popovich, PG;Guan, Z;Mcallister, C;Corcoran, SE;Kaspar, B;Michele Basso, D;Otero, JJ;Kirsch, C;Davis, IC;Croce, CM;Michaille, JJ;Tili, E; | MiR-155 deletion reduces ischemia-induced paralysis in an aortic aneurysm repair mouse model: Utility of immunohistochemistry and histopathology in understanding etiology of spinal cord paralysis | Ann Diagn Pathol | 29966831 | The degree of permeability was determined by measuring the fluorescence of the receiver plate well solution. 2.5. MicroRNA in situ hybridization (ISH). ISH for miR-155 was performed by Phylogeny Inc. (Powell, OH), as previously described [16] | Full Paper |
2018 | Dufu, K;Patel, M;Oksenberg, D;Cabrales, P; | GBT440 improves red blood cell deformability and reduces viscosity of sickle cell blood under deoxygenated conditions | Clin. Hemorheol. Microcirc. | 29660913 | The study was approved by the IRB of the University of North Carolina (UNC) Sickle Cell program [IRB # 88-0343 (GCRC-101)]. Blood from trait individuals (AS) collected in sodium citrate vacutainers was purchased from Conversant Bio, Inc. (Huntsville, | Full Paper |
2018 | Smyth, E;Thuss-Patience, PC; | Immune Checkpoint Inhibition in Gastro-Oesophageal Cancer | Oncol Res Treat | 29705787 | gastro-oesophageal cancer. Unlike the studies with nivolumab, in the KEYNOTE- 12 patients were selected for PD-L1-positive status; however, a different antibody (QualTek 22C3) and scoring system were used. Eligibility was | Full Paper |
2018 | Yuan, Z;Huang, W;Liu, S;Xu, P;Dunham, R;Liu, Z; | Historical demography of common carp estimated from individuals collected from various parts of the world using the pairwise sequentially markovian coalescent approach | Genetica | 29299799 | ; Qiagen). Genome re-sequencing and sequence alignment. DNA library preparation and sequencing were conducted at HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA) following manufacturer's instructions | Full Paper |
2018 | Vakulskas, CA;Dever, DP;Rettig, GR;Turk, R;Jacobi, AM;Collingwood, MA;Bode, NM;McNeill, MS;Yan, S;Camarena, J;Lee, CM;Park, SH;Wiebking, V;Bak, RO;Gomez-Ospina, N;Pavel-Dinu, M;Sun, W;Bao, G;Porteus, MH;Behlke, MA; | A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cells | Nature medicine | 30082871 | ere used from either fresh cord blood (CB) (kindly provided by Binns Family program for Cord Blood Research), isolated from the peripheral blood of SCD patients, or frozen plerixafor mobilized CD34+ (AllCells). HSPCs were cultured at 250,000-500,000 | Full Paper |
2018 | Good, Z;Sarno, J;Jager, A;Samusik, N;Aghaeepour, N;Simonds, EF;White, L;Lacayo, NJ;Fantl, WJ;Fazio, G;Gaipa, G;Biondi, A;Tibshirani, R;Bendall, SC;Nolan, GP;Davis, KL; | Single-cell developmental classification of B cell precursor acute lymphoblastic leukemia at diagnosis reveals predictors of relapse | Nature medicine | 29505032 | Reagents and methods relevant to this work can also be found in the Life Sciences Reporting Summary. Fresh human bone marrow was obtained from healthy adult donors (n = 5; 3 females: 20, 27, 44 y.o., 2 males: 26, 28 y.o., average: 29 y.o.; AllCells, | Full Paper |
2018 | Deng, M;Gui, X;Kim, J;Xie, L;Chen, W;Li, Z;He, L;Chen, Y;Chen, H;Luo, W;Lu, Z;Xie, J;Churchill, H;Xu, Y;Zhou, Z;Wu, G;Yu, C;John, S;Hirayasu, K;Nguyen, N;Liu, X;Huang, F;Li, L;Deng, H;Tang, H;Sadek, AH;Zhang, L;Huang, T;Zou, Y;Chen, B;Zhu, H;Arase, H;Xia, N;Jiang, Y;Collins, R;You, MJ;Homsi, J;Unni, N;Lewis, C;Chen, GQ;Fu, YX;Liao, XC;An, Z;Zheng, J;Zhang, N;Zhang, CC; | LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltration | Nature | 30333625 | ockout was validated by flow cytometry. Leukemia cell and T cell co-culture assay In the co-culture assay, human T cells (5 × 104 per well) isolated from health donor peripheral blood (PB009-1-0, Allcells) were mixed with irradiated (28 Gy) indicate | Full Paper |
2018 | Roth, TL;Puig-Saus, C;Yu, R;Shifrut, E;Carnevale, J;Li, PJ;Hiatt, J;Saco, J;Krystofinski, P;Li, H;Tobin, V;Nguyen, DN;Lee, MR;Putnam, AL;Ferris, AL;Chen, JW;Schickel, JN;Pellerin, L;Carmody, D;Alkorta-Aranburu, G;Del Gaudio, D;Matsumoto, H;Morell, M;Mao, Y;Cho, M;Quadros, RM;Gurumurthy, CB;Smith, B;Haugwitz, M;Hughes, SH;Weissman, JS;Schumann, K;Esensten, JH;May, AP;Ashworth, A;Kupfer, GM;Greeley, SAW;Bacchetta, R;Meffre, E;Roncarolo, MG;Romberg, N;Herold, KC;Ribas, A;Leonetti, MD;Marson, A; | Reprogramming human T cell function and specificity with non-viral genome targeting | Nature | 29995861 | Additional leukapheresis products from healthy donors were collected either under UCLA Institutional Review Board (IRB) approval #10-001598 or purchased from AllCells, LLC. All Save | Full Paper |
2018 | Butler, A;Hoffman, P;Smibert, P;Papalexi, E;Satija, R; | Integrating single-cell transcriptomic data across different conditions, technologies, and species | Nature biotechnology | 29608179 | We therefore repeated the original experiment, where PBMCs from a healthy human donor (ALLCELLS) were cultured with RPMI medium supplemented with 10% FBS and stimulated Save | Full Paper |
2018 | Neal, JT;Li, X;Zhu, J;Giangarra, V;Grzeskowiak, CL;Ju, J;Liu, IH;Chiou, SH;Salahudeen, AA;Smith, AR;Deutsch, BC;Liao, L;Zemek, AJ;Zhao, F;Karlsson, K;Schultz, LM;Metzner, TJ;Nadauld, LD;Tseng, YY;Alkhairy, S;Oh, C;Keskula, P;Mendoza-Villanueva, D;De La Vega, FM;Kunz, PL;Liao, JC;Leppert, JT;Sunwoo, JB;Sabatti, C;Boehm, JS;Hahn, WC;Zheng, GXY;Davis, MM;Kuo, CJ; | Organoid Modeling of the Tumor Immune Microenvironment | Cell | 30550791 | PBMCs, Pan T and CD19 + B cells used for the technical validation part of the immune profiling solution were obtained from AllCells (Catalog #: PB001, PB009-1F and PB010-0). Tumor Save | Full Paper |
2018 | Buenrostro, JD;Corces, MR;Lareau, CA;Wu, B;Schep, AN;Aryee, MJ;Majeti, R;Chang, HY;Greenleaf, WJ; | Integrated Single-Cell Analysis Maps the Continuous Regulatory Landscape of Human Hematopoietic Differentiation | Cell | 29706549 | Healthy adult bone marrow allcells https://www.allcells.com/products/whole-bone-marrow- Samples were collected commercially from allcells. Further information about the donors Save | Full Paper |
2018 | Su, R;Dong, L;Li, C;Nachtergaele, S;Wunderlich, M;Qing, Y;Deng, X;Wang, Y;Weng, X;Hu, C;Yu, M;Skibbe, J;Dai, Q;Zou, D;Wu, T;Yu, K;Weng, H;Huang, H;Ferchen, K;Qin, X;Zhang, B;Qi, J;Sasaki, AT;Plas, DR;Bradner, JE;Wei, M;Marcucci, G;Jiang, X;Mulloy, JC;Jin, J;He, C;Chen, J; | R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling | Cell | 29249359 | R-2-hydroxyglutarate (R-2HG), produced at high levels by mutant isocitrate dehydrogenase 1/2 (IDH1/2) enzymes, was reported as an oncometabolite. We show here that R-2HG also Save | Full Paper |
2018 | Choi, HW;Suwanpradid, J;Kim, IH;Staats, HF;Haniffa, M;MacLeod, AS;Abraham, SN; | Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesicles | Science (New York, N.Y.) | 30409859 | 100 U/ml penicillin, and 100 μg/ml streptomycin was utilized. In brief, purified peripheral blood CD34+ peripheral blood cells (Allcells) were suspended at 5×105 cells/ml in supplemented IMDM containing 100 ng/ml recombinant human (rh) SCF, 10 ng/ml | Full Paper |
2018 | Ting, PY;Parker, AE;Lee, JS;Trussell, C;Sharif, O;Luna, F;Federe, G;Barnes, SW;Walker, JR;Vance, J;Gao, MY;Klock, HE;Clarkson, S;Russ, C;Miraglia, LJ;Cooke, MP;Boitano, AE;McNamara, P;Lamb, J;Schmedt, C;Snead, JL; | Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cells | Nature methods | 30297964 | AllCells and Lonza also obtained samples from outside sources with documented IRB compliance. Lot numbers can be found in the Nature Research Reporting Summary. Save | Full Paper |
2018 | Jenkins, RW;Aref, AR;Lizotte, PH;Ivanova, E;Stinson, S;Zhou, CW;Bowden, M;Deng, J;Liu, H;Miao, D;He, MX;Walker, W;Zhang, G;Tian, T;Cheng, C;Wei, Z;Palakurthi, S;Bittinger, M;Vitzthum, H;Kim, JW;Merlino, A;Quinn, M;Venkataramani, C;Kaplan, JA;Portell, A;Gokhale, PC;Phillips, B;Smart, A;Rotem, A;Jones, RE;Keogh, L;Anguiano, M;Stapleton, L;Jia, Z;Barzily-Rokni, M;Cañadas, I;Thai, TC;Hammond, MR;Vlahos, R;Wang, ES;Zhang, H;Li, S;Hanna, GJ;Huang, W;Hoang, MP;Piris, A;Eliane, JP;Stemmer-Rachamimov, AO;Cameron, L;Su, MJ;Shah, P;Izar, B;Thakuria, M;LeBoeuf, NR;Rabinowits, G;Gunda, V;Parangi, S;Cleary, JM;Miller, BC;Kitajima, S;Thummalapalli, R;Miao, B;Barbie, TU;Sivathanu, V;Wong, J;Richards, WG;Bueno, R;Yoon, CH;Miret, J;Herlyn, M;Garraway, LA;Van Allen, EM;Freeman, GJ;Kirschmeier, PT;Lorch, JH;Ott, PA;Hodi, FS;Flaherty, KT;Kamm, RD;Boland, GM;Wong, KK;Dornan, D;Paweletz, CP;Barbie, DA; | Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids | Cancer discovery | 29101162 | Freshly isolated human CD4+ and CD8+ T cells were obtained from AllCells. Cells were spun down and resuspended in serum free X-vivo15 media (Lonza Walkersville, Inc.) supplemented with 5 ng/mL IL17 and incubated overnight at 37°C. Cells were plated o | Full Paper |
2018 | Ryu, JK;Rafalski, VA;Meyer-Franke, A;Adams, RA;Poda, SB;Rios Coronado, PE;Pedersen, LØ;Menon, V;Baeten, KM;Sikorski, SL;Bedard, C;Hanspers, K;Bardehle, S;Mendiola, AS;Davalos, D;Machado, MR;Chan, JP;Plastira, I;Petersen, MA;Pfaff, SJ;Ang, KK;Hallenbeck, KK;Syme, C;Hakozaki, H;Ellisman, MH;Swanson, RA;Zamvil, SS;Arkin, MR;Zorn, SH;Pico, AR;Mucke, L;Freedman, SB;Stavenhagen, JB;Nelson, RB;Akassoglou, K; | Fibrin-targeting immunotherapy protects against neuroinflammation and neurodegeneration | Nature immunology | 30323343 | wed by an incubation of 6.88 μM CD11b I-domain for 2 h at 25 ˚C. Cell Culture. Primary microglia and BMDMs were isolated, cultured, and treated with fibrin or LPS as previously described16. PBMCs (AllCells) (2×106 cells/mL) were plated in RPMI-1640 | Full Paper |
2018 | Chorzalska, A;Morgan, J;Ahsan, N;Treaba, DO;Olszewski, AJ;Petersen, M;Kingston, N;Cheng, Y;Lombardo, K;Schorl, C;Yu, X;Zini, R;Pacilli, A;Tepper, A;Coburn, J;Hryniewicz-Jankowska, A;Zhao, TC;Oancea, E;Reagan, JL;Liang, O;Kotula, L;Quesenberry, PJ;Gruppuso, PA;Manfredini, R;Vannucchi, AM;Dubielecka, PM; | Bone marrow-specific loss of ABI1 induces myeloproliferative neoplasm with features resembling human myelofibrosis | Blood | 30213875 | CD34 + cells were isolated from the bone marrow of patients with PMF or from healthy marrow purchased from AllCells (Alameda, CA). Granulocytes were isolated from peripheral Save | Full Paper |
2018 | Cairo, M;Parsons, S;Braniecki, S;Talano, J;Moore, T;Shi, Q;Keever-Taylor, C;Weinberg, R;Grossman, B;Dozor, A;Friedman, D;McKinstry, R;Verbsky, J;Moorthy, C;Weidner, R;Rodday, A;Ayello, J;Semidei-Pomales, M;Flower, A;Chu, Y;Morris, E;Mahanti, H;Fabricatore, S;Militano, O;Klejmont, L;van de Ven, C;Baxter-Lowe, L;Vichinsky, E;Walters, M;Shenoy, S; | Significantly Improved Long Term Health Related Quality of Life (HRQL) and Neurocognition Following Familial Haploidentical Stem Cell Transplantation (HISCT) Utilizing CD34 Enrichment and Mononuclear (CD3) Addback in High Risk Patients with Sickle Cell Disease (SCD) | Blood | ViaCord Processing Lab: Other: Medical Director; AllCells Inc.: Other: Medical Director. Shenoy: Novartis, Vertex, Bluebird Bio: Honoraria, Membership on an entity's Board of Directors or advisory committees | Full Paper | |
2018 | Jamieson, CHM;Millan, MT;Creasey, AA;Lomax, G;Donohoe, ME;Walters, MC;Abedi, M;Bota, DA;Zaia, JA;Adams, JS; | CIRM Alpha Stem Cell Clinics: Collaboratively Addressing Regenerative Medicine Challenges | Cell stem cell | 29859170 | Declaration of Interests All authors have CIRM funding to declare. M.C.W. is a Consultant for Bioverativ, Inc., Bluebird Bio, Inc., and Sangamo Biotherapeutics and a Medical Director for AllCells, Inc. and ViaCord Processing Lab | Full Paper |
2018 | Cesana, M;Guo, MH;Cacchiarelli, D;Wahlster, L;Barragan, J;Doulatov, S;Vo, LT;Salvatori, B;Trapnell, C;Clement, K;Cahan, P;Tsanov, KM;Sousa, PM;Tazon-Vega, B;Bolondi, A;Giorgi, FM;Califano, A;Rinn, JL;Meissner, A;Hirschhorn, JN;Daley, GQ; | A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development | Cell stem cell | 29625070 | Human CD34 + cells (Lonza and AllCells) from all sources were obtained as viable frozen states. The ages of the individuals used for the profiling and treatments are as follows: FL- Save | Full Paper |
2018 | Kao, YR;Chen, J;Narayanagari, SR;Todorova, TI;Aivalioti, MM;Ferreira, M;Ramos, PM;Pallaud, C;Mantzaris, I;Shastri, A;Bussel, JB;Verma, A;Steidl, U;Will, B; | Thrombopoietin receptor-independent stimulation of hematopoietic stem cells by eltrombopag | Science translational medicine | 30209246 | Healthy cryopreserved human bone marrow cells were purchased from AllCells and ReachBio Research Labs; fresh unprocessed bone marrow cells were from Lonza. Samples of Save | Full Paper |
2018 | Kou, X;Xu, X;Chen, C;Sanmillan, ML;Cai, T;Zhou, Y;Giraudo, C;Le, A;Shi, S; | The Fas/Fap-1/Cav-1 complex regulates IL-1RA secretion in mesenchymal stem cells to accelerate wound healing | Science translational medicine | 29540618 | Human bone marrow aspirates from healthy human adult volunteers (20 to 35 years of age) were purchased from AllCells LLC. Human GMSCs and SMSCs were isolated from the Save | Full Paper |
2018 | Lai, D;Chen, M;Su, J;Liu, X;Rothe, K;Hu, K;Forrest, DL;Eaves, CJ;Morin, GB;Jiang, X; | PP2A inhibition sensitizes cancer stem cells to ABL tyrosine kinase inhibitors in BCR-ABL+ human leukemia | Science translational medicine | 29437150 | Overcoming drug resistance and targeting leukemic stem cells (LSCs) remain major challenges in curing BCR-ABL + human leukemia. Using an advanced drug/proliferation screen, we Save | Full Paper |
2018 | Ryan, DE;Taussig, D;Steinfeld, I;Phadnis, SM;Lunstad, BD;Singh, M;Vuong, X;Okochi, KD;McCaffrey, R;Olesiak, M;Roy, S;Yung, CW;Curry, B;Sampson, JR;Bruhn, L;Dellinger, DJ; | Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAs | Nucleic acids research | 29216382 | Nucleofector kit (V4SP-3096). Cells were harvested at 48 hr post-transfection. Mobilized human peripheral blood CD34-positive hematopoietic stem and progenitor cells (CD34+ HSPCs) were purchased from AllCells (Alameda, CA) and thawed per manufacturer | Full Paper |
2018 | Syed, SA;Beurel, E;Loewenstein, DA;Lowell, JA;Craighead, WE;Dunlop, BW;Mayberg, HS;Dhabhar, F;Dietrich, WD;Keane, RW;de Rivero Vaccari, JP;Nemeroff, CB; | Defective Inflammatory Pathways in Never-Treated Depressed Patients Are Associated with Poor Treatment Response | Neuron | 30146307 | PBMCs from a non-hispanic white female healthy donor (age 50, weight: 142 lbs, height 68 in) unmedicated for a week, were freshly isolated at ALLCELLS. No other information Save | Full Paper |
2018 | Lei, L;Chen, H;Xue, W;Yang, B;Hu, B;Wei, J;Wang, L;Cui, Y;Li, W;Wang, J;Yan, L;Shang, W;Gao, J;Sha, J;Zhuang, M;Huang, X;Shen, B;Yang, L;Chen, J; | APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks | Nature structural & molecular biology | 29323274 | Cryopreserved human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells, LLC (PB003F). CD8+ T cells were pre-enriched from the PBMCs with Easysep Human CD8+ T cell enrichment kit (19053, Stemcell Technologies) according to the man | Full Paper |
2018 | Stoeckius, M;Zheng, S;Houck-Loomis, B;Hao, S;Yeung, BZ;Mauck, WM;Smibert, P;Satija, R; | Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomics | Genome biology | 30567574 | urther enable multiplexing strategies to generalize to diverse experiments regardless of species, tissue, or technology. Methods PBMC genotyping Peripheral blood mononuclear cells were obtained from AllCells (USA). Genomic DNA was purified using the | Full Paper |
2018 | Xu, Y;Zhao, W;Olson, SD;Prabhakara, KS;Zhou, X; | Alternative splicing links histone modifications to stem cell fate decision | Genome biology | 30217220 | After reaching ~ 70% confluence, the adherent cells were harvested at the passage 1 (P1) hAT-MSC/AEY239. hBM-MSCs were isolated from commercially available fresh human bone marrow (hBM) aspirates (AllCells, Emeryville, CA, USA) and expanded following | Full Paper |
2018 | Salas, LA;Koestler, DC;Butler, RA;Hansen, HM;Wiencke, JK;Kelsey, KT;Christensen, BC; | An optimized library for reference-based deconvolution of whole-blood biospecimens assayed using the Illumina HumanMethylationEPIC BeadArray | Genome biology | 29843789 | urity cell subtypes (neutrophils (Neu), monocytes (Mono), B lymphocytes (Bcells), T helper lymphocytes (CD4T), T cytotoxic lymphocytes (CD8T), and natural killer lymphocytes (NK)) were purchased from AllCells corporation (Alameda, CA, USA) and STEMC | Full Paper |
2018 | Lim, SH;Kathuria, H;Tan, JJY;Kang, L; | 3D printed drug delivery and testing systems - a passing fad or the future? | Advanced drug delivery reviews | 29778901 | Primary human dental pulp stem cells (DPSC) from a single donor (DPF003, AllCells, USA) were cultured in a complete growth media containing Dulbecco's Modified Eagle's Medium, supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. C | Full Paper |
2018 | Marabissi, D;Bartoli, G;Micciullo, L;Fantacci, R; | Energy efficient multicast beamforming in ultra dense networks | 2018 IEEE Wireless Communications and Networking Conference (WCNC) | This figure clearly shows the better behavior of our method in comparison with static approaches (ie, OnlyMacro, AllCells). Moreover, we can see that even if sub-optimum, our method is Save | Full Paper | |
2018 | Cao, L;Pauthner, M;Andrabi, R;Rantalainen, K;Berndsen, Z;Diedrich, JK;Menis, S;Sok, D;Bastidas, R;Park, SR;Delahunty, CM;He, L;Guenaga, J;Wyatt, RT;Schief, WR;Ward, AB;Yates, JR;Burton, DR;Paulson, JC; | Differential processing of HIV envelope glycans on the virus and soluble recombinant trimer | Nature communications | 30209313 | Primary PBMC samples were obtained through the commercial vendor AllCells (Emeryville, CA The leukapak sample was obtained from a commercial vendor (AllCells) using a protocol Save | Full Paper |
2018 | Mezger, A;Klemm, S;Mann, I;Brower, K;Mir, A;Bostick, M;Farmer, A;Fordyce, P;Linnarsson, S;Greenleaf, W; | High-throughput chromatin accessibility profiling at single-cell resolution | Nature communications | 30194434 | , and B cells were isolated from whole blood (AllCells, CA, USA) using EasySep Direct Human All human cells were obtained from AllCells with explicit consent to publish data for broad Save | Full Paper |
2018 | Lyu, G;Guan, Y;Zhang, C;Zong, L;Sun, L;Huang, X;Huang, L;Zhang, L;Tian, XL;Zhou, Z;Tao, W; | TGF-β signaling alters H4K20me3 status via miR-29 and contributes to cellular senescence and cardiac aging | Nature communications | 29967491 | The culture medium was MEM supplemented with 10% FBS (GIBCO), and the culture dish was gelatin-coated before inoculation of primary human umbilical vascular endothelial cells (HUVECs) purchased from ALLCELLS Co. Ltd., Shanghai, China. Cells were test | Full Paper |
2018 | Xu, L;Gordon, R;Farmer, R;Pattanayak, A;Binkowski, A;Huang, X;Avram, M;Krishna, S;Voll, E;Pavese, J;Chavez, J;Bruce, J;Mazar, A;Nibbs, A;Anderson, W;Li, L;Jovanovic, B;Pruell, S;Valsecchi, M;Francia, G;Betori, R;Scheidt, K;Bergan, R; | Precision therapeutic targeting of human cancer cell motility | Nature communications | 29934502 | od cells, hemoglobin, and platelets. Hematopoietic stem cell colony formation assay Fourteen-day colony formation assays were conducted as described by us39, using human cord blood CD34+ stem cells (AllCells Inc.), using MethoCultExpress colony gro | Full Paper |
2018 | Yang, R;Yu, T;Kou, X;Gao, X;Chen, C;Liu, D;Zhou, Y;Shi, S; | Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoter | Nature communications | 29858571 | (PFA, Sigma-Aldrich) after 16 days of culturing. Clusters with more than 50 cells were counted as colonies under microscopy. Isolation and culture of human BMMSCs We purchased human bone marrow from AllCells LLC (Alameda, CA, USA), which were aspira | Full Paper |
2018 | Lummertz da Rocha, E;Rowe, RG;Lundin, V;Malleshaiah, M;Jha, DK;Rambo, CR;Li, H;North, TE;Collins, JJ;Daley, GQ; | Reconstruction of complex single-cell trajectories using CellRouter | Nature communications | 29497036 | es) would act as perturbations that could be explored by CLR to identify putative regulatory relationships. In vitro erythroid differentiation Mobilized peripheral blood CD34+ stem/progenitor cells (AllCells) were obtained from three independent don | Full Paper |
2018 | Ravi, R;Noonan, KA;Pham, V;Bedi, R;Zhavoronkov, A;Ozerov, IV;Makarev, E;V Artemov, A;Wysocki, PT;Mehra, R;Nimmagadda, S;Marchionni, L;Sidransky, D;Borrello, IM;Izumchenko, E;Bedi, A; | Bifunctional immune checkpoint-targeted antibody-ligand traps that simultaneously disable TGFβ enhance the efficacy of cancer immunotherapy | Nature communications | 29467463 | )(4-8-week old) were irradiated at 200 cGy, followed by adoptive transfer of human BM CD34 + cells (7 × 10 4 per mouse) from a normal donor (HLA-matched to the tumor) (ALLCELLS) Save | Full Paper |
2018 | Bak, RO;Dever, DP;Porteus, MH; | CRISPR/Cas9 genome editing in human hematopoietic stem cells | Nature protocols | 29370156 | Fresh or frozen CD34 + human HSPCs (either acquired per institutional protocol or purchased from various commercial sources, including AllCells (CB-derived, cat. no. CB008F; mPB- Save | Full Paper |
2018 | Adine, C;Ng, KK;Rungarunlert, S;Souza, GR;Ferreira, JN; | Engineering innervated secretory epithelial organoids by magnetic three-dimensional bioprinting for stimulating epithelial growth in salivary glands | Biomaterials | 30025245 | Human dental pulp stem cells (hDPSCs) were commercially obtained from AllCells (Alameda, CA, USA) from three different donors. These stem cells were derived from the pulp tissues of exfoliated deciduous teeth, primary incisors, or permanent third mol | Full Paper |
2018 | Jiang, L;Zhang, W;Wei, L;Zhou, Q;Yang, G;Qian, N;Tang, Y;Gao, Y;Jiang, X; | Early effects of parathyroid hormone on vascularized bone regeneration and implant osseointegration in aged rats | Biomaterials | 29960821 | Human umbilical vein endothelial cells (HUVECs) (Allcells, Shanghai, China) were cultured and their in vitro recruitment and angiogenesis abilities were measured. PTH (1-34) was added at a concentration of 10 nM in the lower chambers of 24-well trans | Full Paper |
2018 | Odegaard, JI;Vincent, JJ;Mortimer, S;Vowles, JV;Ulrich, BC;Banks, KC;Fairclough, SR;Zill, OA;Sikora, M;Mokhtari, R;Abdueva, D;Nagy, RJ;Lee, CE;Kiedrowski, LA;Paweletz, CP;Eltoukhy, H;Lanman, RB;Chudova, DI;Talasaz, A; | Validation of a Plasma-Based Comprehensive Cancer Genotyping Assay Utilizing Orthogonal Tissue- and Plasma-Based Methodologies | Clinical cancer research : an official journal of the American Association for Cancer Research | 29691297 | Contrived analytic samples were generated using similarly prepared cfDNA from healthy donors (AllCells, Inc.) and cfDNA isolated as above from the culture supernatant of model cell Save | Full Paper |
2018 | L'Italien, L;Orozco, O;Abrams, T;Cantagallo, L;Connor, A;Desai, J;Ebersbach, H;Gelderblom, H;Hoffmaster, K;Lees, E;Maacke, H;Schleyer, S;Skegro, D;Lee-Hoeflich, ST; | Mechanistic Insights of an Immunological Adverse Event Induced by an Anti-KIT Antibody Drug Conjugate and Mitigation Strategies | Clinical cancer research : an official journal of the American Association for Cancer Research | 29615457 | Human peripheral blood cell-derived mast cells (PBC-MCs) were generated from mobilized peripheral blood CD34 + cells isolated from healthy donors (AllCells #mPB017F). Mast cells Save | Full Paper |
2018 | Cromer, MK;Vaidyanathan, S;Ryan, DE;Curry, B;Lucas, AB;Camarena, J;Kaushik, M;Hay, SR;Martin, RM;Steinfeld, I;Bak, RO;Dever, DP;Hendel, A;Bruhn, L;Porteus, MH; | Global Transcriptional Response to CRISPR/Cas9-AAV6-Based Genome Editing in CD34+ Hematopoietic Stem and Progenitor Cells | Molecular therapy : the journal of the American Society of Gene Therapy | 30005866 | CD34+ HSPCs used for gene expression microarrays were obtained as frozen stock derived from mobilized peripheral blood thawed according to manufacturer’s instructions (AllCells). For follow-up experiments, CD34+ HSPCs were obtained fresh from cord bl | Full Paper |
2018 | Ligtenberg, MA;Pico de Coaña, Y;Shmushkovich, T;Yoshimoto, Y;Truxova, I;Yang, Y;Betancur-Boissel, M;Eliseev, AV;Wolfson, AD;Kiessling, R; | Self-Delivering RNAi Targeting PD-1 Improves Tumor-Specific T Cell Functionality for Adoptive Cell Therapy of Malignant Melanoma | Molecular therapy : the journal of the American Society of Gene Therapy | 29735366 | ation (Ficoll-Paque Plus; GE Healthcare). T cells were purified using the Pan T cell isolation kit (Miltenyi Biotec) following manufacturer’s instructions. Peripheral blood CD3+ Pan T cells from AllCells (PB009-1F) were used for sdRNA validation and | Full Paper |
2018 | Georgiadis, C;Preece, R;Nickolay, L;Etuk, A;Petrova, A;Ladon, D;Danyi, A;Humphryes-Kirilov, N;Ajetunmobi, A;Kim, D;Kim, JS;Qasim, W; | Long Terminal Repeat CRISPR-CAR-Coupled "Universal" T Cells Mediate Potent Anti-leukemic Effects | Molecular therapy : the journal of the American Society of Gene Therapy | 29605708 | CliniMACS Prodigy using tubing set TS520 (130-019-002, Miltenyi Biotec) and used cryopreserved leukapheresis harvests (Allcells, Alameda, USA) cultured in TexMACS good manufacturing practice (GMP) medium (170-076-307, Miltenyi Biotec) supplemented wi | Full Paper |
2018 | Heffner, GC;Bonner, M;Christiansen, L;Pierciey, FJ;Campbell, D;Smurnyy, Y;Zhang, W;Hamel, A;Shaw, S;Lewis, G;Goss, KA;Garijo, O;Torbett, BE;Horton, H;Finer, MH;Gregory, PD;Veres, G; | Prostaglandin E2 Increases Lentiviral Vector Transduction Efficiency of Adult Human Hematopoietic Stem and Progenitor Cells | Molecular therapy : the journal of the American Society of Gene Therapy | 29102562 | CD34-enriched mPB samples from healthy human donors were obtained from AllCells (Emeryville, CA, USA), Key Biologics (Memphis, TN, USA), and primary patients under Save;Methods Cell Culture Research-grade GFP-, globin-, and ALDP-containing LVVs wer | Full Paper |
2018 | Mokhtari, S;Colletti, E;Yin, W;Sanada, C;Lamar, Z;Simmons, PJ;Walker, S;Bishop, C;Atala, A;Zanjani, ED;Porada, CD;Almeida-Porada, G; | A human bone marrow mesodermal-derived cell population with hemogenic potential | Leukemia | 29467489 | al bone tissues were obtained from Advanced Bioscience Resources (Alameda, CA). Human adult bone marrow (BM) was obtained from healthy adult donors after informed consent, or from commercial sources (AllCells, LLC, Alameda, CA). Fetal and adult tissu | Full Paper |
2018 | Tan, YT;Ye, L;Xie, F;Beyer, AI;Muench, MO;Wang, J;Chen, Z;Liu, H;Chen, SJ;Kan, YW; | Respecifying human iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells with a single factor | Proceedings of the National Academy of Sciences of the United States of America | 29386396 | Frozen human PB mobilized CD34+ cells and PB mononuclear cells were purchased from AllCells (www.allcells.com). The protocols involving human iPSCs were approved by Human Research Committee and Human Gamete, Embryo and Stem Cells Research Committee ( | Full Paper |
2018 | Li, Y;Carpenito, C;Wang, G;Surguladze, D;Forest, A;Malabunga, M;Murphy, M;Zhang, Y;Sonyi, A;Chin, D;Burtrum, D;Inigo, I;Pennello, A;Shen, L;Malherbe, L;Chen, X;Hall, G;Haidar, JN;Ludwig, DL;Novosiadly, RD;Kalos, M; | Discovery and preclinical characterization of the antagonist anti-PD-L1 monoclonal antibody LY3300054 | Journal for immunotherapy of cancer | 29712568 | s analyzed using GraphPad Prism software [25]. Mixed leukocyte reaction (MLR) CD14+ monocytes were isolated from frozen human peripheral blood mononuclear cells (PBMC) obtained from a healthy donor (AllCells, Alameda, CA) with Human Monocyte Isolati | Full Paper |
2018 | Liu, Y;Jing, H;Kou, X;Chen, C;Liu, D;Jin, Y;Lu, L;Shi, S; | PD-1 is required to maintain stem cell properties in human dental pulp stem cells | Cell death and differentiation | 29472716 | Isolation and culture of human BMMSCs Human bone marrow aspirates from healthy human adult volunteers (20-35 years of age) were purchased from AllCells LLC. hBMMSCs were cultured as reported previously [40] | Full Paper |
2018 | Willingham, SB;Ho, PY;Hotson, A;Hill, C;Piccione, EC;Hsieh, J;Liu, L;Buggy, JJ;McCaffery, I;Miller, RA; | A2AR Antagonism with CPI-444 Induces Antitumor Responses and Augments Efficacy to Anti-PD-(L)1 and Anti-CTLA-4 in Preclinical Models | Cancer immunology research | 30131376 | PBMCs were purchased from AllCells and either used fresh or cryopreserved for future use. Fresh PBMCs were cultured at a density of 10 7 cells/mL, and cryopreserved cells were Save | Full Paper |
2018 | Siriwon, N;Kim, YJ;Siegler, E;Chen, X;Rohrs, JA;Liu, Y;Wang, P; | CAR-T Cells Surface-Engineered with Drug-Encapsulated Nanoparticles Can Ameliorate Intratumoral T-cell Hypofunction | Cancer immunology research | 29720380 | Frozen human peripheral blood mononuclear cells (PBMC) were obtained from AllCells. Thawed PBMCs were cultured in T-cell medium (TCM) containing X-VIVO 15 serum-free Save | Full Paper |
2018 | Oeller, M;Laner-Plamberger, S;Hochmann, S;Ketterl, N;Feichtner, M;Brachtl, G;Hochreiter, A;Scharler, C;Bieler, L;Romanelli, P;Couillard-Despres, S;Russe, E;Schallmoser, K;Strunk, D; | Selection of Tissue Factor-Deficient Cell Transplants as a Novel Strategy for Improving Hemocompatibility of Human Bone Marrow Stromal Cells | Theranostics | 29507631 | Three additional BM samples (10 mL each) were purchased via CellSystems from ALLCELLS (USA). The mean age of the male BM donors was 34 years (range 20-46) and of the Save | Full Paper |
2018 | de la Puente, P;Luderer, MJ;Federico, C;Jin, A;Gilson, RC;Egbulefu, C;Alhallak, K;Shah, S;Muz, B;Sun, J;King, J;Kohnen, D;Salama, NN;Achilefu, S;Vij, R;Azab, AK; | Enhancing proteasome-inhibitory activity and specificity of bortezomib by CD38 targeted nanoparticles in multiple myeloma | Journal of controlled release : official journal of the Controlled Release Society | 29196043 | Bone Marrow mononuclear cells (BM MNCs) from 5 different patient samples were purchased from Allcells (Alameda, CA). Peripheral blood mononuclear cells (PB MNCs) were Save | Full Paper |
2018 | Chen, Y;Mao, P;Snijders, AM;Wang, D; | Senescence chips for ultrahigh-throughput isolation and removal of senescent cells | Aging cell | 29336105 | Fresh human whole blood from healthy donors collected within 24 hr was purchased from AllCells Inc. (Alameda, CA). Human MSCs were purchased from Lonza (Lonza, Swiss). Save | Full Paper |
2018 | Charlesworth, CT;Camarena, J;Cromer, MK;Vaidyanathan, S;Bak, RO;Carte, JM;Potter, J;Dever, DP;Porteus, MH; | Priming Human Repopulating Hematopoietic Stem and Progenitor Cells for Cas9/sgRNA Gene Targeting | Molecular therapy. Nucleic acids | 30195800 | CD34 + HSPCs derived from mobilized peripheral blood were purchased from AllCells, and CD34 + HSPCs derived from cord blood were provided from donors under informed consent Save;ollowing the manufacturer’s instructions. Titers were measured after b | Full Paper |
2018 | Vaidyanathan, S;Azizian, KT;Haque, AKMA;Henderson, JM;Hendel, A;Shore, S;Antony, JS;Hogrefe, RI;Kormann, MSD;Porteus, MH;McCaffrey, AP; | Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification | Molecular therapy. Nucleic acids | 30195789 | CD34 + HSPCs derived from mobilized peripheral blood donated by male donors were purchased from AllCells (Alemeda, CA). Cells were thawed according to the manufacturer's Save;tection System luminometer (Promega, E8032) with a 10-s integration per w | Full Paper |
2018 | Yoshinaga, T;Seki, T;Miki, S;Miyamoto, T;Suyama-Kagitani, A;Kawauchi-Miki, S;Kobayashi, M;Sato, A;Stewart, E;Underwood, M;Fujiwara, T; | Novel secondary mutations C56S and G149A confer resistance to HIV-1 integrase strand transfer inhibitors | Antiviral research | 29410019 | kanamycin sulfate. Peripheral blood mononuclear cells (PBMCs; AllCells, Alameda, CA) were cultured in RPMI 1640 medium supplemented with 10% FBS, 60 μg/mL of kanamycin sulfate, and 10 μg/mL of phytohemagglutinin (PHA)-M (Roche, Indianapolis, IN) for | Full Paper |
2018 | Li, S;Hong, Z;Wang, Z;Li, F;Mei, J;Huang, L;Lou, X;Zhao, S;Song, L;Chen, W;Wang, Q;Liu, H;Cai, Y;Yu, H;Xu, H;Zeng, G;Wang, Q;Zhu, J;Liu, X;Tan, N;Wang, C; | The Cyclopeptide Astin C Specifically Inhibits the Innate Immune CDN Sensor STING | Cell reports | 30566866 | hPBMCs and IMR-90 (female) cells were purchased from AllCells and Cobioer Bioscience, respectively. The gender information of hPBMCs is not available from the supplier. The Save | Full Paper |
2018 | Osorio, FG;Rosendahl Huber, A;Oka, R;Verheul, M;Patel, SH;Hasaart, K;de la Fonteijne, L;Varela, I;Camargo, FD;van Boxtel, R; | Somatic Mutations Reveal Lineage Relationships and Age-Related Mutagenesis in Human Hematopoiesis | Cell reports | 30485801 | nd Cat# 302005; RRID:AB_314205 CD11c-FITC BioLegend Cat# 301603; RRID:AB_314173 ------------------------- Biological Samples ------------------------- Whole bone marrow samples A,C,D,E AllCells ABM001 Whole bone marrow sample B B | Full Paper |
2018 | Zhu, YP;Padgett, L;Dinh, HQ;Marcovecchio, P;Blatchley, A;Wu, R;Ehinger, E;Kim, C;Mikulski, Z;Seumois, G;Madrigal, A;Vijayanand, P;Hedrick, CC; | Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone Marrow | Cell reports | 30157427 | Fresh BM samples of anonymous healthy adult donors were obtained from AllCells, Inc. (Alameda, CA). The cells were stained for either flow cytometry or FACS-sorting following protocols described in the Flow Cytometry and Cell Sorting section | Full Paper |
2018 | Schaer, DA;Beckmann, RP;Dempsey, JA;Huber, L;Forest, A;Amaladas, N;Li, Y;Wang, YC;Rasmussen, ER;Chin, D;Capen, A;Carpenito, C;Staschke, KA;Chung, LA;Litchfield, LM;Merzoug, FF;Gong, X;Iversen, PW;Buchanan, S;de Dios, A;Novosiadly, RD;Kalos, M; | The CDK4/6 Inhibitor Abemaciclib Induces a T Cell Inflamed Tumor Microenvironment and Enhances the Efficacy of PD-L1 Checkpoint Blockade | Cell reports | 29539425 | Breast cancer cell lines MCF-7 and MDA-MB-468 (American Type Culture Collection [ATCC]) were treated with DMSO and abemaciclib (500 nM) for 8 days. RNA was isolated as described above. Jurkat T cells (ATCC) or primary human T cells (AllCells) were st | Full Paper |
2018 | Shi, J;Zhang, M;Zhang, L;Deng, H; | Epigallocatechin-3-gallate attenuates microcystin-LR-induced apoptosis in human umbilical vein endothelial cells through activation of the NRF2/HO-1 pathway | Environmental pollution (Barking, Essex : 1987) | 29679944 | HUVECs were obtained from Allcells Technology (Shanghai, China) and seeded in complete medium (HUVEC-004, AllCells Technology) in a humidified atmosphere of 5% CO 2 at 37 Save | Full Paper |
2018 | Wienert, B;Shin, J;Zelin, E;Pestal, K;Corn, JE; | In vitro-transcribed guide RNAs trigger an innate immune response via the RIG-I pathway | PLoS biology | 30011268 | HSPCs from mobilized peripheral blood (Allcells) were thawed and cultured in StemSpan SFEM medium (StemCell Technologies) supplemented with StemSpan CC110 cocktail ( Save | Full Paper |
2018 | Yang, W;Sun, Z;Hua, C;Wang, Q;Xu, W;Deng, Q;Pan, Y;Lu, L;Jiang, S; | Chidamide, a histone deacetylase inhibitor-based anticancer drug, effectively reactivates latent HIV-1 provirus | Microbes and infection | 29126877 | J-Lat A2 cells [25] (obtained from NIH AIDS Reagent Program), J-Lat C11 cells [26], PBMCs (Allcells), ACH-2 cells [27] and U1 cells [28] were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS) and 1% Pen/strep in a 37 °C incubator contain | Full Paper |
2018 | Ito, R;Maruoka, S;Soda, K;Katano, I;Kawai, K;Yagoto, M;Hanazawa, A;Takahashi, T;Ogura, T;Goto, M;Takahashi, R;Toyoshima, S;Okayama, Y;Izuhara, K;Gon, Y;Hashimoto, S;Ito, M;Nunomura, S; | A humanized mouse model to study asthmatic airway inflammation via the human IL-33/IL-13 axis | JCI insight | 30385714 | Commercially available human cord blood-derived CD34 + cells (AllCells LLC or Lonza) were used in this study. The cells were prepared according to the manufacturer's instructions. Save | Full Paper |
2018 | Antebi, B;Rodriguez, LA;Walker, KP;Asher, AM;Kamucheka, RM;Alvarado, L;Mohammadipoor, A;Cancio, LC; | Short-term physiological hypoxia potentiates the therapeutic function of mesenchymal stem cells | Stem cell research & therapy | 30305185 | arrow MSCs (pMSCs) were isolated from mononuclear cells (MNCs) obtained from bone marrow aspirates, as previously described [39]. Human bone marrow MSCs (hMSCs) were isolated from MNCs purchased from AllCells LLC (Emeryville, CA, USA). MNCs were cult | Full Paper |
2018 | Senyuk, V;Patel, P;Mahmud, N;Rondelli, D; | Blockade of TNFα to Improve Human CD34+ Cell Repopulating Activity in Allogeneic Stem Cell Transplantation | Frontiers in immunology | 30723481 | Healthy donor G-CSF mobilized peripheral blood stem cells (PBSC) from AllCells (Alameda, CA) and PB cells from healthy volunteers were utilized in this study. Mononuclear cells ( Save | Full Paper |
2018 | Cui, C;Su, M;Lin, Y;Lai, L; | A CD300c-Fc Fusion Protein Inhibits T Cell Immunity | Frontiers in immunology | 30498497 | peripheral blood CD3 + Pan T Cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan-T labeling system were purchased from ALLCELLS, Save | Full Paper |
2018 | Cobbold, SP;Adams, E;Howie, D;Waldmann, H; | CD4+ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium Development | Frontiers in immunology | 29967616 | t CV (%) is indicated. In all experiments, P values of | Full Paper |
2018 | Burton, OT;Epp, A;Fanny, ME;Miller, SJ;Stranks, AJ;Teague, JE;Clark, RA;van de Rijn, M;Oettgen, HC; | Tissue-Specific Expression of the Low-Affinity IgG Receptor, FcγRIIb, on Human Mast Cells | Frontiers in immunology | 29928276 | genic for membrane-bound human stem cell factor (SCF) [NOD.Cg-PrkdcscidIl2rgtm1Wjl Tg(PGK1-KITLG*220)441Daw/SzJ] were engrafted with 5 × 104 CD34+ hematopoietic stem cells (HSC) from cord blood (AllCells) for 16-24 weeks. Wild-type BALB/c, C57BL/6J, | Full Paper |
2018 | White, CH;Beliakova-Bethell, N;Lada, SM;Breen, MS;Hurst, TP;Spina, CA;Richman, DD;Frater, J;Magiorkinis, G;Woelk, CH; | Transcriptional Modulation of Human Endogenous Retroviruses in Primary CD4+ T Cells Following Vorinostat Treatment | Frontiers in immunology | 29706951 | rials and Methods Isolation of Primary CD4+ T Cells For subsequent total RNA-Seq analysis, cryopreserved primary CD4+ T cells that were viably frozen were obtained from four different healthy donors (AllCells, Inc., Emeryville, CA, USA) and thawed in | Full Paper |
2018 | Bhaskaran, N;Liu, Z;Saravanamuthu, SS;Yan, C;Hu, Y;Dong, L;Zelenka, P;Zheng, L;Bletsos, V;Harris, R;Harrington, B;Weinberg, A;Thiele, CJ;Ye, F;Pandiyan, P; | Identification of Casz1 as a Regulatory Protein Controlling T Helper Cell Differentiation, Inflammation, and Immunity | Frontiers in immunology | 29467767 | done at NIAID, NIH under an approved protocol, and in compliance with the NIAID Institutional Animal Care and Use Committee’s guidelines. Human cells were obtained from commercially available PBMC (AllCells). Reagents and Antibodies Purified or fluo | Full Paper |
2018 | Hanazawa, A;Ito, R;Katano, I;Kawai, K;Goto, M;Suemizu, H;Kawakami, Y;Ito, M;Takahashi, T; | Generation of Human Immunosuppressive Myeloid Cell Populations in Human Interleukin-6 Transgenic NOG Mice | Frontiers in immunology | 29456539 | ined in the Central Institute for Experimental Animals (CIEA) under specific pathogen-free conditions. Transplantation of Human HSCs Human umbilical-cord-blood-derived CD34+ HSCs were purchased from Allcells (Alameda, CA, USA). For transplantation, | Full Paper |
2018 | Seto, AG;Beatty, X;Lynch, JM;Hermreck, M;Tetzlaff, M;Duvic, M;Jackson, AL; | Cobomarsen, an oligonucleotide inhibitor of miR-155, co-ordinately regulates multiple survival pathways to reduce cellular proliferation and survival in cutaneous T-cell lymphoma | British journal of haematology | 30125933 | miR‐155, a microRNA associated with poor prognosis in lymphoma and leukaemia, has been implicated in the progression of mycosis fungoides ( MF ), the most common form of Save | Full Paper |
2018 | Makar, T;Nimmagadda, VK;Guda, PR;Hampton, B;Huang, W;Kane, MA;Fishman, PS;Pessac, B;Bever, CT;Trisler, D; | A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditions | Stem cell research & therapy | 29720239 | Human buffy coat cells and CD34 + cells from peripheral blood were purchased from ALLCELLS (Alameda, CA, USA). Human buffy coat cells were obtained by differential Save | Full Paper |
2018 | Fujisawa, K;Hara, K;Takami, T;Okada, S;Matsumoto, T;Yamamoto, N;Sakaida, I; | Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cells | Stem cell research & therapy | 29625581 | MSCs were derived from bone marrow cells (AllCells, Alameda, CA,USA) and were used at passage (P)4. Mononuclear cells (MNCs) were purchased from Lonza, Tokyo, Japan. Flow Save | Full Paper |
2018 | Thekkeparambil Chandrabose, S;Sriram, S;Subramanian, S;Cheng, S;Ong, WK;Rozen, S;Kasim, NHA;Sugii, S; | Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming | Stem cell research & therapy | 29559008 | The list of commercial (Lonza and Allcells) and patient-derived dental cells used in this manuscript can be found in Additional file 1: Table S1. DPSCs were grown in vitro in Poietics Save | Full Paper |
2018 | Johnson, HWB;Lowe, E;Anderl, JL;Fan, A;Muchamuel, T;Bowers, S;Moebius, DC;Kirk, C;McMinn, DL; | Required Immunoproteasome Subunit Inhibition Profile for Anti-Inflammatory Efficacy and Clinical Candidate KZR-616 ((2 S,3 R)- N-(( S)-3-(Cyclopent-1-en-1-yl)-1-(( R)-2-methyloxiran-2-yl)-1-oxopropan-2-yl)-3-hydroxy-3-(4-methoxyphenyl)-2-(( S)-2-(2-morpholinoacetamido)propanamido)propenamide) | Journal of medicinal chemistry | 30380863 | Cryopreserved peripheral blood mononuclear cells (PBMCs) from normal healthy volunteers were purchased from AllCells (Alameda, CA) and cultured in RPMI 1640 media (with 300 mg/L l-glutamine) supplemented with 5% heat-inactivated FBS, 100 IU penicilli | Full Paper |
2018 | Vazquez, ML;Kaila, N;Strohbach, JW;Trzupek, JD;Brown, MF;Flanagan, ME;Mitton-Fry, MJ;Johnson, TA;TenBrink, RE;Arnold, EP;Basak, A;Heasley, SE;Kwon, S;Langille, J;Parikh, MD;Griffin, SH;Casavant, JM;Duclos, BA;Fenwick, AE;Harris, TM;Han, S;Caspers, N;Dowty, ME;Yang, X;Banker, ME;Hegen, M;Symanowicz, PT;Li, L;Wang, L;Lin, TH;Jussif, J;Clark, JD;Telliez, JB;Robinson, RP;Unwalla, R; | Identification of N-{cis-3-[Methyl(7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino]cyclobutyl}propane-1-sulfonamide (PF-04965842): A Selective JAK1 Clinical Candidate for the Treatment of Autoimmune Diseases | Journal of medicinal chemistry | 29298069 | Cryopreserved human bone marrow CD34+ cells (cat. no. ABM022F) were purchased from Allcells. Frozen bone marrow CD34+ cells were thawed, washed once with IMEM, resuspended in Stem Span medium (cat. no. 09600; STEMCELL Technologies, Vancouver, Canada) | Full Paper |
2018 | Xu, H;Liao, C;Zuo, P;Liu, Z;Ye, BC; | Magnetic-Based Microfluidic Device for On-Chip Isolation and Detection of Tumor-Derived Exosomes | Analytical chemistry | 30234974 | CD63 antibody (anti-CD63) from Biolegend (USA), biotin-labeled mouse antihuman CD9 antibody (anti-CD9) from Allcells Biotech Co., Ltd. (Shanghai, China), and HRP-conjugated antimouse IgG from QF Biosciences Co., Ltd. (Shanghai, China). MagCapture Exo | Full Paper |
2018 | Xu, H;Liao, C;Liu, Y;Ye, BC;Liu, B; | Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living Cells | Analytical chemistry | 29508999 | Human umbilical vein endothelial cells (HUVECs) (ALLCELLS, Shanghai) were cultured in a humidified incubator (95% air with 5% CO2) at 37 °C with a complete medium designed for optimal growth of endothelial cells in vitro. A 6 mm thick PDMS containing | Full Paper |
2018 | Yasinska, IM;Gonçalves Silva, I;Sakhnevych, SS;Ruegg, L;Hussain, R;Siligardi, G;Fiedler, W;Wellbrock, J;Bardelli, M;Varani, L;Raap, U;Berger, S;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV; | High mobility group box 1 (HMGB1) acts as an "alarmin" to promote acute myeloid leukaemia progression | Oncoimmunology | 29872582 | lemented with 10% foetal bovine serum, penicillin (50 IU/ml) and streptomycin sulphate (50 µg/ml). Primary human AML mononuclear blasts (AML-PB001 F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordanc | Full Paper |
2018 | Lam, J;Bellayr, IH;Marklein, RA;Bauer, SR;Puri, RK;Sung, KE; | Functional Profiling of Chondrogenically Induced Multipotent Stromal Cell Aggregates Reveals Transcriptomic and Emergent Morphological Phenotypes Predictive of Differentiation Capacity | Stem cells translational medicine | 30084545 | rived MSCs were obtained from eight different donors purchased from RoosterBio (RB9, RB12, RB14, RB16; Frederick, MD) at passage 1 (P1), Lonza (8F560, 167696; Walkersville, MD) at passage 2 (P2), and AllCells (PCBM1632, PCBM1641; Alameda, CA) at P2 ( | Full Paper |
2018 | George, MJ;Prabhakara, K;Toledano-Furman, NE;Wang, YW;Gill, BS;Wade, CE;Olson, SD;Cox, CS; | Clinical Cellular Therapeutics Accelerate Clot Formation | Stem cells translational medicine | 30070065 | Bone marrow derived MSCs (BM MSCs) were isolated from commercially available fresh human bone marrow aspirates (AllCells, Emeryville, CA) or obtained from fresh bone marrow Save | Full Paper |
2018 | Marcellino, BK;Hoffman, R;Tripodi, J;Lu, M;Kosiorek, H;Mascarenhas, J;Rampal, RK;Dueck, A;Najfeld, V; | Advanced forms of MPNs are accompanied by chromosomal abnormalities that lead to dysregulation of TP53 | Blood advances | 30563882 | Normal bone marrow MNCs were obtained from AllCells (Alameda, CA). RNA was extracted from MNCs, using RNeasy mini kit (Fisher Scientific, Waltham, MA), and was reverse Save | Full Paper |
2018 | Wei, Y;Zheng, H;Bao, N;Jiang, S;Bueso-Ramos, CE;Khoury, J;Class, C;Lu, Y;Lin, K;Yang, H;Ganan-Gomez, I;Starczynowski, DT;Do, KA;Colla, S;Garcia-Manero, G; | KDM6B overexpression activates innate immune signaling and impairs hematopoiesis in mice | Blood advances | 30275007 | BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). CD34 + cells were isolated using MicroBead Kit (Miltenyi Biotec). For colony formation, BM CD34 + Save | Full Paper |
2018 | Witt, RG;Kreger, EM;Buckman, LB;Moradi, PW;Ho, PT;Derderian, SC;Tsai, P;Baker, C;Schramm, N;Cleary, R;Garcia, JV;MacKenzie, TC; | Systemic multilineage engraftment in mice after in utero transplantation with human hematopoietic stem cells | Blood advances | 29344586 | Human cord blood CD34 + cells were either purchased from AllCells (Alameda, CA) or purified from cord blood collected at time of delivery from normal-term infants. CD34 + cells were Save | Full Paper |
2018 | Murray, C;Miwa, H;Dhar, M;Park, DE;Pao, E;Martinez, J;Kaanumale, S;Loghin, E;Graf, J;Raddassi, K;Kwok, WW;Hafler, D;Puleo, C;Di Carlo, D; | Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratcheting | Lab on a chip | 30039125 | Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) were purchased from ALLCELLs (Alameda, CA, USA; Cat No. PB003F) and stored at −180°C in vapor phase liquid nitrogen until ready for experimental use. Cells were thawed by placing cryovial in 37 | Full Paper |
2018 | Romero-Masters, JC;Ohashi, M;Djavadian, R;Eichelberg, MR;Hayes, M;Bristol, JA;Ma, S;Ranheim, EA;Gumperz, J;Johannsen, EC;Kenney, SC; | An EBNA3C-deleted Epstein-Barr virus (EBV) mutant causes B-cell lymphomas with delayed onset in a cord blood-humanized mouse model | PLoS pathogens | 30125329 | Commercially available CD34-depleted human cord blood mononuclear cells (AllCells) were infected with 5000 Green Raji Units of wild-type or mutant EBV strains in vitro for 1 ½ hours Save | Full Paper |
2018 | Miller, AZ;Satchie, A;Tannenbaum, AP;Nihal, A;Thomson, JA;Vereide, DT; | Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal Transition | Stem cell reports | 29320761 | scribed in Figure 5 as a means to transiently label transfected cells. Sources of Primary Cells Frozen primary CD34+ cells purified from umbilical cord blood or adult bone marrow were obtained from AllCells (for cord blood, products CB005F and CB009 | Full Paper |
2018 | Tsai, HC;Velichko, S;Lee, S;Wu, R; | Cholera toxin enhances interleukin-17A production in both CD4+ and CD8+ cells via a cAMP/protein kinase A-mediated interleukin-17A promoter activation | Immunology | 29377102 | Peripheral blood mononuclear cells were purchased from Allcells, LLC (Alameda, CA) or isolated from the leukopak filters from the Delta Blood Bank in Stockton, CA. Total CD4 + T Save | Full Paper |
2018 | Yang, R;Liu, Y;Yu, T;Liu, D;Shi, S;Zhou, Y;Zhou, Y; | Hydrogen sulfide maintains dental pulp stem cell function via TRPV1-mediated calcium influx | Cell death discovery | 30062050 | 00 μg/ml streptomycin and passaged, as previously reported27. Human bone marrow MSCs BMMSC culture Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells (Emeryville, CA, USA). Human bone marrow proge | Full Paper |
2018 | Zhou, Z;Shao, T;Qin, M;Miao, X;Chang, Y;Sheng, W;Wu, F;Yu, Y; | The effects of autophagy on vascular endothelial cells induced by airborne PM2.5 | Journal of environmental sciences (China) | 29628085 | In this study, human umbilical vein endothelial cells (HUVECs) were obtained from Allcells, China. The cells were routinely maintained in an HUVEC complete culture medium (Allcells, Save | Full Paper |
2018 | Figueroa, I;Leipold, D;Leong, S;Zheng, B;Triguero-Carrasco, M;Fourie-O'Donohue, A;Kozak, KR;Xu, K;Schutten, M;Wang, H;Polson, AG;Kamath, AV; | Prediction of non-linear pharmacokinetics in humans of an antibody-drug conjugate (ADC) when evaluation of higher doses in animals is limited by tolerability: Case study with an anti-CD33 ADC | mAbs | 29757698 | 61 Samples included normal human whole blood donors (n = 6 from Genentech, Inc. South San Francisco, CA; for phenotyping lymphocyte and myeloid subsets), normal human bone marrow donors (n = 5 from ALLCells, Alameda, CA; for phenotyping hematopoietic | Full Paper |
2018 | Jiang, N;Wang, Y;Yin, Y;Wei, R;Ying, G;Li, B;Qiu, T;van Rijn, P;Tian, G;Yan, Q;Dai, H;Busscher, H;Li, S;Yetisen, A;Yang, X; | bFGF and Poly-RGD Cooperatively Establish Biointerface for Stem Cell Adhesion, Proliferation, and Differentiation | Advanced Materials Interfaces | hBMMSCs Culture: Freshly isolated human bone marrow mononuclear cells obtained from a 23-year-old donor were purchased from ALLCELLS (Emeryville, CA, US), and cultured with α-minimum essential media (MEM)/15% FBS/glutamine (2 mmol L−1)/penicillin (10 | Full Paper | |
2018 | Hoff, FW;Hu, CW;Qiu, Y;Ligeralde, A;Yoo, SY;Mahmud, H;de Bont, ESJM;Qutub, AA;Horton, TM;Kornblau, SM; | Recognition of Recurrent Protein Expression Patterns in Pediatric Acute Myeloid Leukemia Identified New Therapeutic Targets | Molecular cancer research : MCR | 29669821 | patients (57 pre-B ALL and 16 T-ALL, patient demographics are presented in Supplemental Table S2), 10 cryopreserved normal BM CD34+ (AllCells, Almeda CA), and 127 leukemic cell line samples. The methods and validation of the technique, as well as the | Full Paper |
2018 | Bates, AM;Fischer, CL;Abhyankar, VP;Johnson, GK;Guthmiller, JM;Progulske-Fox, A;Brogden, KA; | Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-B | International journal of molecular sciences | 30544510 | ls, GE keratinocytes, and T-cells were chosen for this experiment due to their presence in the periodontium and their innate immune functions. Human monocyte-derived immature myeloid dendritic cells (AllCells, Alameda, CA, USA) were thawed, washed wi | Full Paper |
2018 | Chen, Q;Smith, S;Fong, T;Shirazian, A;Lee, J;Chapman, L;Tong, J;Vaz, W; | Practical considerations for sourcing clinical-grade human tissue to support development and production of emerging commercial cellular therapies | Cytotherapy | Q. Chen, S. Smith, T. Fong, A. Shirazian, J. Lee, L. Chapman, J. Tong, W. Vaz Commercial, AllCells, LLC, Alameda, California, United States The rapid clinical advancement and commercial manufacturing of cellular therapeutics pose numerous industrial | Full Paper | |
2018 | Okragly, AJ;Morin, SM;DeRosa, D;Martin, AP;Johnson, KW;Johnson, MP;Benschop, RJ; | Human mast cells release the migraine-inducing factor pituitary adenylate cyclase-activating polypeptide (PACAP) | Cephalalgia : an international journal of headache | 29103295 | Human umbilical cord blood-derived mast cells were produced by in vitro differentiation of positively selected cord blood CD34+ progenitor cells (Allcells) as previously described (37,38). Cells were cultured in Serum Free Expansion Media (SFEM Cat. | Full Paper |
2018 | Oberst, MD;Augé, C;Morris, C;Kentner, S;Mulgrew, K;McGlinchey, K;Hair, J;Hanabuchi, S;Du, Q;Damschroder, M;Feng, H;Eck, S;Buss, N;de Haan, L;Pierce, AJ;Park, H;Sylwester, A;Axthelm, MK;Picker, L;Morris, NP;Weinberg, A;Hammond, SA; | Potent Immune Modulation by MEDI6383, an Engineered Human OX40 Ligand IgG4P Fc Fusion Protein | Molecular cancer therapeutics | 29545330 | Ligation of OX40 (CD134, TNFRSF4) on activated T cells by its natural ligand (OX40L, CD252, TNFSF4) enhances cellular survival, proliferation, and effector functions such as cytokine Save | Full Paper |
2018 | Fujisawa, K;Takami, T;Okada, S;Hara, K;Matsumoto, T;Yamamoto, N;Yamasaki, T;Sakaida, I; | Analysis of Metabolomic Changes in Mesenchymal Stem Cells on Treatment with Desferrioxamine as a Hypoxia Mimetic Compared with Hypoxic Conditions | Stem cells (Dayton, Ohio) | 29577517 | Cells and Cell Culturing BMSCs were obtained from bone marrow cells (age: 34, male) purchased from AllCells (Alameda, CA), and BMSCs were proliferated using passage 4 and passage 16. BMSCs were confirmed to be CD45 (-), CD11b (-), CD90 (+), CD73 (+), | Full Paper |
2018 | Fujii, S;Miura, Y;Fujishiro, A;Shindo, T;Shimazu, Y;Hirai, H;Tahara, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T; | Graft-Versus-Host Disease Amelioration by Human Bone Marrow Mesenchymal Stromal/Stem Cell-Derived Extracellular Vesicles Is Associated with Peripheral Preservation of Naive T Cell Populations | Stem cells (Dayton, Ohio) | 29239062 | Human MSCs were isolated from BM samples purchased from AllCells (Alameda, CA) using our previously published method [21, 22]. BM samples were obtained from three different Save | Full Paper |
2018 | Harting, MT;Srivastava, AK;Zhaorigetu, S;Bair, H;Prabhakara, KS;Toledano Furman, NE;Vykoukal, JV;Ruppert, KA;Cox, CS;Olson, SD; | Inflammation-Stimulated Mesenchymal Stromal Cell-Derived Extracellular Vesicles Attenuate Inflammation | Stem cells (Dayton, Ohio) | 29076623 | Human mesenchymal stromal cells (hMSCs) were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using Save | Full Paper |
2018 | Meader, E;Barta, T;Melguizo-Sanchis, D;Tilgner, K;Montaner, D;El-Harouni, AA;Armstrong, L;Lako, M; | Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAs | Stem cells (Dayton, Ohio) | 29047185 | and TaqMan MicroRNA Assays were used for the qRT‐PCR (all from Thermo Fisher Scientific) RNU44 and RNU48 were chosen as internal controls. CD34+ human adult bone marrow and CD34+ cord blood cells (AllCells, Alameda, CA, USA) were used as positive con | Full Paper |
2018 | Wang, J;Li, T;Feng, J;Li, L;Wang, R;Cheng, H;Yuan, Y; | Kaempferol protects against gamma radiation-induced mortality and damage via inhibiting oxidative stress and modulating apoptotic molecules in vivo and vitro | Environmental toxicology and pharmacology | 29705372 | To investigate the potential protective effect of kaempferol, a representative flavonoid, against radiation induced mortality and injury in vivo and vitro.C57BL/6 male mice and human Save | Full Paper |
2018 | Garbuzova-Davis, S;Haller, E;Navarro, S;Besong, TE;Boccio, KJ;Hailu, S;Khatib, M;Sanberg, PR;Appel, SH;Borlongan, CV; | Transplantation of human bone marrow stem cells into symptomatic ALS mice enhances structural and functional blood-spinal cord barrier repair | Experimental neurology | 30172620 | Accumulating evidence shows alterations in the blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) in ALS patients and in animal models of disease, mainly by endothelial Save | Full Paper |
2018 | Huang, L;Zhang, X;Ma, X;Zhang, D;Li, D;Feng, J;Pan, X;Lü, J;Wang, X;Liu, X; | Berberine alleviates endothelial glycocalyx degradation and promotes glycocalyx restoration in LPS-induced ARDS | International immunopharmacology | 30308440 | (Shanghai, China) and grown to confluence in 25 cm 2 flasks supplemented with complete culture medium, which was provided by AllCells. The cells were incubated under 95% Save | Full Paper |
2018 | Romero, Z;DeWitt, M;Walters, MC; | Promise of gene therapy to treat sickle cell disease | Expert opinion on biological therapy | 30324810 | MC Walters is the Medical Director of AllCells, Inc and ViaCord Processing Lab; Consultant for Sangamo Therapeutics, INc, bluebird bio, Inc., and Bioverativ, Inc. The authors have no other relevant affiliations or financial involvement with any organ | Full Paper |
2018 | Hu, Y;Yao, X;Liu, Q;Wang, Y;Liu, R;Cui, S;Ding, J; | Left-Right Symmetry or Asymmetry of Cells on Stripe-Like Micropatterned Material Surfaces | Chinese Journal of Chemistry | rMSCs were bought from Allcells Biological Technology, Shanghai. hMSCs were from Cyagen Biosciences, Suzhou. HFFs and HUVECs were products of Bogoo Biological Technology, Shanghai. The cells of rMSCs were incubated in minimum essential medium α (MEM | Full Paper | |
2018 | Yuan, X;Han, L;Fu, P;Zeng, H;Lv, C;Chang, W;Runyon, RS;Ishii, M;Han, L;Liu, K;Fan, T;Zhang, W;Liu, R; | Cinnamaldehyde accelerates wound healing by promoting angiogenesis via up-regulation of PI3K and MAPK signaling pathways | Laboratory investigation; a journal of technical methods and pathology | 29463877 | Cell culture and animal maintenance Primary HUVECs were purchased from AllCells (Emeryville, CA, USA). With the exception of the homocysteine-rescue tube assay, each batch of HUVECs was examined using flow cytometry and was shown to be 99% CD31 posit | Full Paper |
2018 | Huang, T;Mathieu, M;Lee, S;Wang, X;Kee, YS;Bevers, JJ;Ciferri, C;Estavez, A;Wong, M;Chiang, NY;Nakamura, G;Brezski, RJ; | Molecular characterization of human anti-hinge antibodies derived from single-cell cloning of normal human B cells | The Journal of biological chemistry | 29191832 | PBMCs were isolated from a Leuko Pak (AllCells) using Ficoll-Paque gradient fractionation (GE Healthcare). PBMCs were then frozen in 90% fetal bovine serum (FBS) supplemented with 10% dimethyl sulfoxide (DMSO) and stored in liquid nitrogen until thaw | Full Paper |
2018 | Smith, AJ;Graves, B;Child, R;Rice, PJ;Ma, Z;Lowman, DW;Ensley, HE;Ryter, KT;Evans, JT;Williams, DL; | Immunoregulatory Activity of the Natural Product Laminarin Varies Widely as a Result of Its Physical Properties | Journal of immunology (Baltimore, Md. : 1950) | 29246954 | PBMCs obtained via leukapheresis were purchased from AllCells (Alameda, CA) and cryopreserved in 50% RPMI 1640 with 40% FBS/10% DMSO, after extensive washing in 1× PBS. Save | Full Paper |
2018 | Yang, C;Huan, Z;Wang, X;Wu, C;Chang, J; | 3D Printed Fe Scaffolds with HA Nanocoating for Bone Regeneration | ACS biomaterials science & engineering | 33418749 | initial density of 1× 104 rabbit bone marrow mesenchymal stem cells (rBMSCs) (Allcells, Silicon Valley, CA, USA) in 48-well tissue culture plates and then incubated at 37 °C wiht 5% CO2. After 24 h, the cell culture medium was changed for the first t | Full Paper |
2018 | Piao, XM;Gao, F;Zhu, JX;Wang, LJ;Zhao, X;Li, X;Sheng, MM;Zhang, Y; | Cucurbitacin B inhibits tumor angiogenesis by triggering the mitochondrial signaling pathway in endothelial cells | International journal of molecular medicine | 29717773 | HUVECs and HepG2 cells were obtained from Allcells, LLC (Shanghai, China). CuB (purity 99%) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China). DMEM/F-12, fetal bovine serum (FBS), was from Gibco (Carlsbad, CA, USA). EGM-2 complete medi | Full Paper |
2018 | Zhu, H;Yu, B;Yang, L; | Exogenous and Endogenous Stem Cells for Skeletal Regeneration | Stem cells international | 30581472 | HUVECs were purchased from AllCells, Ltd. (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth factors [19]. To investigate the Save | Full Paper |
2018 | Zhang, M;Yu, W;Niibe, K;Zhang, W;Egusa, H;Tang, T;Jiang, X; | The Effects of Platelet-Derived Growth Factor-BB on Bone Marrow Stromal Cell-Mediated Vascularized Bone Regeneration | Stem cells international | 30515221 | HUVECs were purchased from AllCells, Ltd. (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth factors [19]. To investigate the Save | Full Paper |
2018 | Kwak, J;Choi, SJ;Oh, W;Yang, YS;Jeon, HB;Jeon, ES; | Cobalt Chloride Enhances the Anti-Inflammatory Potency of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells through the ERK-HIF-1α-MicroRNA-146a-Mediated Signaling Pathway | Stem cells international | 30254683 | 103 cells/well) were seeded and maintained at 37°C in a humidified incubator for 2-4 hr and then cocultured with responder peripheral blood mononuclear cells (PBMCs; 1 × 105 cells/well; AllCells, Boston, MA, USA) and stimulator PBMCs (1 × 105 cells/ | Full Paper |
2018 | Chen, B;Ni, Y;Liu, J;Zhang, Y;Yan, F; | Bone Marrow-Derived Mesenchymal Stem Cells Exert Diverse Effects on Different Macrophage Subsets | Stem cells international | 30140291 | y directed activation. Western blot was used to detect the expression of iNOS and Arg-1 in the cells to further verify the M1 and M2 polarization. 2.5. Preparation of MSC-CM BMMSC was purchased from AllCells, LLC (Emeryville, CA), and MSCs at passag | Full Paper |
2018 | Jiang, J;Xu, K;Wang, L;Xin, W;Zhao, G;Huang, M;Li, S;Luan, X;Fang, J; | Pharmacology study of a chimeric decoy receptor trap fusion protein on retina neovascularization by dual blockage of VEGF and FGF-2 | European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences | 29715501 | Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from AllCells (Shanghai, China) and maintained in a complete HUVEC medium (Cat# H-004, AllCells) | Full Paper |
2018 | Yen, J;Fiorino, M;Liu, Y;Paula, S;Clarkson, S;Quinn, L;Tschantz, WR;Klock, H;Guo, N;Russ, C;Yu, VWC;Mickanin, C;Stevenson, SC;Lee, C;Yang, Y; | TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem Cells | Scientific reports | 30389991 | Cryopreserved bone marrow derived CD34 + HSPCs were purchased from Lonza or AllCells and thawed according to vendors' instructions. Cells were cultured at 37 C 5% CO 2 in Save | Full Paper |
2018 | Han, SS;Shim, HE;Park, SJ;Kim, BC;Lee, DE;Chung, HM;Moon, SH;Kang, SW; | Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking | Scientific reports | 30181604 | The protocol was approved by the Committee on Biological Research of Korea Institute of Toxicology and Institutional Review Board (P01-201509-41-001). hUCB-EPCs culture hUCB-EPCs were purchased from AllCells (Alameda, CA, USA). hUCB-EPCs culture wer | Full Paper |
2018 | Burgenson, D;Gurramkonda, C;Pilli, M;Ge, X;Andar, A;Kostov, Y;Tolosa, L;Rao, G; | Rapid recombinant protein expression in cell-free extracts from human blood | Scientific reports | 29934577 | anced the expression of Nluc, but RNase inhibitor did not have a significant effect (Fig. 2C,D).Figure 1Production of human leukocyte extract and Nluc expression. (A) Leukopak was purchased form the AllCells, Inc. (B) The cells were clarified by cent | Full Paper |
2018 | Valton, J;Guyot, V;Boldajipour, B;Sommer, C;Pertel, T;Juillerat, A;Duclert, A;Sasu, BJ;Duchateau, P;Poirot, L; | A Versatile Safeguard for Chimeric Antigen Receptor T-Cell Immunotherapies | Scientific reports | 29895885 | Primary T-cells isolated from PBMC (ALLCELLS) were activated and transfected according to previously described methods 13 . Four days after their activation by Dynabeads human T Save | Full Paper |
2018 | Gaudin, A;Tolar, M;Peters, OA; | Lipoxin A4 Attenuates the Inflammatory Response in Stem Cells of the Apical Papilla via ALX/FPR2 | Scientific reports | 29892010 | eated with mitomycin C (Sigma-Aldrich) at the final concentration of 25 μg/mL for 30 min, and then washed 4 times with PBS + 10% Human Serum. Human peripheral blood mononuclear cells (PBMC) (AllCells, Alameda, CA, USA) in dilution ratios 1:1, 1:5 and | Full Paper |
2018 | Lim, SH;Sun, Y;Thiruvallur Madanagopal, T;Rosa, V;Kang, L; | Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular Modification | Scientific reports | 29371611 | 215 nm. A calibration curve was conducted using the respective standard solutions from 1 ppm to 1000 ppm. DPSC Cell Culture Primary human dental pulp stem cells (DPSC) from a single donor (DPF003, AllCells, USA) were cultured in a complete growth me | Full Paper |
2018 | Ruppert, KA;Nguyen, TT;Prabhakara, KS;Toledano Furman, NE;Srivastava, AK;Harting, MT;Cox, CS;Olson, SD; | Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Modify Microglial Response and Improve Clinical Outcomes in Experimental Spinal Cord Injury | Scientific reports | 29323194 | hMSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old male (AllCells, Alameda, CA) using density centrifugation and plastic adherence Save | Full Paper |
2018 | Li, C;Sunderic, K;Nicoll, SB;Wang, S; | Downregulation of Heat Shock Protein 70 Impairs Osteogenic and Chondrogenic Differentiation in Human Mesenchymal Stem Cells | Scientific reports | 29323151 | Human MSCs were isolated from fresh human bone marrow purchased from AllCells (Alameda, CA) according to manufacturer's instructions as previously described 2 and cultured Save | Full Paper |
2018 | Bloom, DD;Reshetylo, S;Nytes, C;Goodsett, CT;Hematti, P; | Blockade of BAFF Receptor BR3 on T Cells Enhances Their Activation and Cytotoxicity | Journal of immunotherapy (Hagerstown, Md. : 1997) | 29309290 | Primary human T cells were obtained from leukopheresis products purchased from AllCells, LLC (Alameda, CA) or Key Biologics, LLC (Memphis, TN). Upon arrival, PBLs were isolated Save | Full Paper |
2018 | Mair, F;Prlic, M; | OMIP-044: 28-color immunophenotyping of the human dendritic cell compartment | Cytometry. Part A : the journal of the International Society for Analytical Cytology | 29356334 | Cryopreserved PBMCs were obtained from AllCells, stained and subsequently aquired on a BD FACSymphony. Optimal PMT voltages were determined by voltage titration experiments and iterative refinement. All reagents were titrated prior to use. For Figure | Full Paper |
2018 | Doering, CB;Denning, G;Shields, JE;Fine, EJ;Parker, ET;Srivastava, A;Lollar, P;Spencer, HT; | Preclinical Development of a Hematopoietic Stem and Progenitor Cell Bioengineered Factor VIII Lentiviral Vector Gene Therapy for Hemophilia A | Human gene therapy | 30160169 | Genetically modified, autologous hematopoietic stem and progenitor cells (HSPCs) represent a new class of genetic medicine. Following this therapeutic paradigm, we are developing a Save | Full Paper |
2018 | Liu, XY;Xu, HX;Li, JK;Zhang, D;Ma, XH;Huang, LN;Lü, JH;Wang, XZ; | Neferine Protects Endothelial Glycocalyx via Mitochondrial ROS in Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome | Frontiers in physiology | 29520236 | Human umbilical vein endothelial cells (HUVECs) were grown to confluence and cultured in 25 cm 2 flasks supplemented with complete culture medium (AllCells, Shanghai, China) in a Save | Full Paper |
2018 | Zeng, Y;Dabay, M;George, V;Seetharaman, S;de Arruda Indig, M;Graminske, S;Kimpel, N;Schmidt, A;Boerner, A;Paradiso, S;Delman, T;Li, Y;Litvak, V;Oreizy, F;Chen, A;Saleminik, M;Mosqueda, F;Lin, A;Judge, K; | Comparison of Flow Cytometric Methods for the Enumeration of Residual Leucocytes in Leucoreduced Blood Products: A Multicenter Study | Cytometry. Part A : the journal of the International Society for Analytical Cytology | 29345745 | Per enrollment needs, leucoreduced PLTs and RBCs with autologous white blood cell sources were also purchased from qualified vendors that produce apheresis PLTs and RBCs through standard procedures (AllCells, LLC, Alameda, CA; Stanford Blood Center, | Full Paper |
2018 | Song, B;Chen, Y;Liu, Y;Wan, C;Zhang, L;Zhang, W; | NPAS2 regulates proliferation of acute myeloid leukemia cells via CDC25A-mediated cell cycle progression and apoptosis | Journal of cellular biochemistry | 30536616 | NHC cell specimens were separated from normal cells in bone marrow bought from AllCells, LLC (Emeryville, CA). Cells were preserved in 5% CO 2 at 37C in RPMI-1640 media Save | Full Paper |
2018 | Lewis, G;Christiansen, L;McKenzie, J;Luo, M;Pasackow, E;Smurnyy, Y;Harrington, S;Gregory, P;Veres, G;Negre, O;Bonner, M; | Staurosporine Increases Lentiviral Vector Transduction Efficiency of Human Hematopoietic Stem and Progenitor Cells | Molecular therapy. Methods & clinical development | 30038935 | CD34-enriched mPB samples from healthy human donors were obtained from AllCells (Emeryville, CA) and Key Biologics (Memphis, TN). Research-grade staurosporine was obtained Save;apeutics. Materials and Methods Cell Culture Research-grade BlaM-conta | Full Paper |
2018 | Mendoza, M;Ballesteros, A;Qiu, Q;Pow Sang, L;Shashikumar, S;Casares, S;Brumeanu, TD; | Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD) | Human vaccines & immunotherapeutics | 29135340 | Briefly, HLA-DRB1*0401 + umbilical cord bloods (UCB) purchased from AllCells and Promocell were enriched for CD34 + stem cells to more than 60% when using EasySep, Human Save | Full Paper |
2018 | Low, EK;Brudvik, E;Kuhlman, B;Wilson, PF;Almeida-Porada, G;Porada, CD; | Microgravity Impairs DNA Damage Repair in Human Hematopoietic Stem/Progenitor Cells and Inhibits Their Differentiation into Dendritic Cells | Stem cells and development | 29901426 | Healthy human BM mononuclear cells (BMMNC) were purchased from AllCells, LLC (Alameda, CA). CD34+ HSC were isolated from BMMNC using anti-human CD34 MicroBeads and the MiniMACS magnetic-based positive-selection system (Miltenyi Biotec, Inc., Auburn, | Full Paper |
2018 | Almeida-Porada, G;Rodman, C;Kuhlman, B;Brudvik, E;Moon, J;George, S;Guida, P;Sajuthi, SP;Langefeld, CD;Walker, SJ;Wilson, PF;Porada, CD; | Exposure of the Bone Marrow Microenvironment to Simulated Solar and Galactic Cosmic Radiation Induces Biological Bystander Effects on Human Hematopoiesis | Stem cells and development | 29698131 | In brief, upon return from BNL, MSC from each irradiation group were overlaid with human HSC (CD34+ BM-derived cells from healthy adult donors, purchased from AllCells, LLC, or Save | Full Paper |
2018 | Sebastian, AA;Kannan, TP;Norazmi, MN;Nurul, AA; | Interleukin-17A promotes osteogenic differentiation by increasing OPG/RANKL ratio in stem cells from human exfoliated deciduous teeth (SHED) | Journal of tissue engineering and regenerative medicine | 29774992 | SHED were purchased from ALLCELLS, Alameda, CA, USA (Catalogue No. DP004F; lot number SHED092211-01). SHED from Passages 4 to 6 were used in the study. SHED were cultured in alpha minimum essential medium (Gibco, USA) supplemented with 10% v/v fetal | Full Paper |
2018 | Gregg, KA;Harberts, E;Gardner, FM;Pelletier, MR;Cayatte, C;Yu, L;McCarthy, MP;Marshall, JD;Ernst, RK; | A lipid A-based TLR4 mimetic effectively adjuvants a Yersinia pestis rF-V1 subunit vaccine in a murine challenge model | Vaccine | 29861179 | Co-stimulatory marker surface expression assays were contracted to and completed by AllCells using standard protocols. Briefly, ex vivo human monocytes were differentiated to Save | Full Paper |
2018 | Kanagasabai, R;Karmahapatra, S;Kientz, CA;Yu, Y;Hernandez, VA;Kania, EE;Yalowich, JC;Elton, TS; | The Novel C-terminal Truncated 90-kDa Isoform of Topoisomerase IIα (TOP2α/90) Is a Determinant of Etoposide Resistance in K562 Leukemia Cells via Heterodimerization with the TOP2α/170 Isoform | Molecular pharmacology | 29514855 | Human cord blood mononuclear cells were purchased from ALLCELLS (Alameda, CA). Deidentified acute myeloid leukemia (AML) patient samples were obtained from The Ohio State Save | Full Paper |
2018 | Liu, L;Puri, N;Raffaele, M;Schragenheim, J;Singh, SP;Bradbury, JA;Bellner, L;Vanella, L;Zeldin, DC;Cao, J;Abraham, NG; | Ablation of soluble epoxide hydrolase reprogram white fat to beige-like fat through an increase in mitochondrial integrity, HO-1-adiponectin in vitro and in vivo | Prostaglandins & other lipid mediators | 30041041 | Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA, USA). After thawing, mononuclear cells were re-suspended in a-minimal essential medium (a-MEM, Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat-in-a | Full Paper |
2018 | Wang, J;Xu, J;Peng, Y;Xiao, Y;Zhu, H;Ding, ZM;Hua, H; | Phosphorylation of extracellular signal-regulated kinase as a biomarker for cannabinoid receptor 2 activation | Heliyon | 30450437 | tal calf serum, 100 U/ml penicillin/streptomycin, and 2 mM l-glutamine at 37 °C in 5% CO2/95% air. 2.2 Human whole blood cell culture Human whole blood cells were purchased from commercial sources (Allcells, Alameda, CA, USA or Zen-Bio, Research Tri | Full Paper |
2018 | Wiredja, DD;Tabler, CO;Schlatzer, DM;Li, M;Chance, MR;Tilton, JC; | Global phosphoproteomics of CCR5-tropic HIV-1 signaling reveals reprogramming of cellular protein production pathways and identifies p70-S6K1 and MK2 as HIV-responsive kinases required for optimal infection of CD4+ T cells | Retrovirology | 29970186 | Normal donor samples were de-identified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated by Save | Full Paper |
2018 | Friesen, WJ;Johnson, B;Sierra, J;Zhuo, J;Vazirani, P;Xue, X;Tomizawa, Y;Baiazitov, R;Morrill, C;Ren, H;Babu, S;Moon, YC;Branstrom, A;Mollin, A;Hedrick, J;Sheedy, J;Elfring, G;Weetall, M;Colacino, JM;Welch, EM;Peltz, SW; | The minor gentamicin complex component, X2, is a potent premature stop codon readthrough molecule with therapeutic potential | PloS one | 30359426 | ). Fold suppression over background was calculated as (aminoglycoside light units/PBS light units) [7]. Cytotoxicity assays Frozen human Peripheral Blood Mononuclear Cells (PBMCs) were obtained from ALLCELLS (Alameda, CA, Cat# PB003F) from a single | Full Paper |
2018 | Caseiro, AR;Ivanova, G;Pedrosa, SS;Branquinho, MV;Georgieva, P;Barbosa, PP;Santos, JD;Magalhães, R;Teixeira, P;Pereira, T;Maurício, AC; | Human umbilical cord blood plasma as an alternative to animal sera for mesenchymal stromal cells in vitro expansion - A multicomponent metabolomic analysis | PloS one | 30304014 | , 29, 47-49]. UC-MSCs and DPSCs were maintained at 37°C and 95% humidified atmosphere with 5% CO2 environment. UC-MSCs were obtained from PromoCell, Cat. C-12971; Lot No. 1112304.2) and DPSCs from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01). | Full Paper |
2018 | Oshima, T;Miyashita, H;Ishimura, Y;Ito, Y;Tanaka, Y;Hori, A;Kokubo, T;Kurokawa, T; | Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey | PloS one | 29723247 | Human peripheral blood mononuclear cells (PBMC) purchased from AllCells, LLC and were cultured in RPMI1640 medium containing 10% fetal bovine serum, 0.1 nM human IL-2 ( Save | Full Paper |
2018 | Del'Guidice, T;Lepetit-Stoffaes, JP;Bordeleau, LJ;Roberge, J;Théberge, V;Lauvaux, C;Barbeau, X;Trottier, J;Dave, V;Roy, DC;Gaillet, B;Garnier, A;Guay, D; | Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cells | PloS one | 29617431 | N). Myoblasts are primary human cells kindly provided by Professor J.P. Tremblay (Université Laval, Quebec, QC, Canada). Normal Peripheral Blood CD56+ NK cells are natural killer cells obtained from Allcells Inc. (Alameda, CA, USA). Cortical cells ar | Full Paper |
2018 | Sunderic, K; | Effects of Thermal Dosing and Ultrasound on Osteogenic Differentiation of Human Mesenchymal Stem Cells in 2D and 3D Culture | Thesis | Human bone marrow explanted from the iliac crest of donors was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the RosetteSep MSC enrichment cocktail (StemCell Technologies, Vancouver, Canada) per manufac | Full Paper | |
2018 | Nair, RR;Piktel, D;Geldenhuys, WJ;Gibson, LF; | Combination of cabazitaxel and plicamycin induces cell death in drug resistant B-cell acute lymphoblastic leukemia | Leukemia research | 30103201 | Primary immune cells CD3 + T cells, CD19 + B cells, peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) were all purchased from ALLCELLS and Save | Full Paper |
2018 | Palasuk, J;Windsor, LJ;Platt, JA;Lvov, Y;Geraldeli, S;Bottino, MC; | Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashion | Clinical oral investigations | 28965247 | Next, the eluates were serially diluted to 32-, 64-, and 128-fold [21]. Human dental pulp stem cells ([hDPSCs] AllCells LLC, Alameda, CA, USA) were cultured under standard conditions in a humidifier incubator (5% CO2/95% air) at 37 °C in low-glucose | Full Paper |
2018 | Sasaki, E;Momose, H;Hiradate, Y;Ishii, KJ;Mizukami, T;Hamaguchi, I; | In vitro marker gene expression analyses in human peripheral blood mononuclear cells: A tool to assess safety of influenza vaccines in humans | Journal of immunotoxicology | 29521144 | PBMC obtained from healthy adult subjects were purchased from AllCells (Alameda, CA) and cultured in complete RPMI, ie RPMI 1640 supplemented with 10% fetal calf serum (FCS), Save | Full Paper |
2018 | Tian, H;Li, S;Yu, K; | DJ‑1 alleviates high glucose‑induced endothelial cells injury via PI3K/Akt‑eNOS signaling pathway | Molecular medicine reports | 29115508 | HUVECs were purchased from AllCells Biotechnology Co., Ltd. (Shanghai, China). The culture medium was DMEM with either 5.5 mM (control group) or 25 mM (HG group) D-glucose, containing 10% fetal bovine serum, penicillin (100 U/ml) and streptomycin (10 | Full Paper |
2018 | Hu, X;Hu, T;Guan, G;Yu, S;Wu, Y;Wang, L; | Control of weft yarn or density improves biocompatibility of PET small diameter artificial blood vessels | Journal of biomedical materials research. Part B, Applied biomaterials | 28456004 | The HUVECs (HUVEC001), provided by Allcells Company (Shanghai, China), were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Gibco) at 37°C in a 5% CO2 incubator. Before se | Full Paper |
2018 | Joslin, J;Gilligan, J;Anderson, P;Garcia, C;Sharif, O;Hampton, J;Cohen, S;King, M;Zhou, B;Jiang, S;Trussell, C;Dunn, R;Fathman, JW;Snead, JL;Boitano, AE;Nguyen, T;Conner, M;Cooke, M;Harris, J;Ainscow, E;Zhou, Y;Shaw, C;Sipes, D;Mainquist, J;Lesley, S; | A Fully Automated High-Throughput Flow Cytometry Screening System Enabling Phenotypic Drug Discovery | SLAS discovery : advancing life sciences R & D | 29843542 | Primary human CD4 T cells were purified using a CD4+ T-cell isolation kit (130-096-533, Miltenyi, Auburn, CA) from cells obtained from leukapheresis of a normal human donor (AllCells Save;reg (T-Regulatory) Screen Primary human CD4 T cells were puri | Full Paper |
2018 | Barve, A;Casson, L;Krem, M;Wunderlich, M;Mulloy, JC;Beverly, LJ; | Comparative utility of NRG and NRGS mice for the study of normal hematopoiesis, leukemogenesis, and therapeutic response | Experimental hematology | 30125602 | Cell-line-derived xenografts (CDXs) or patient-derived xenografts (PDXs) in immune-deficient mice have revolutionized our understanding of normal and malignant human Save | Full Paper |
2018 | Reshetnikova, E;Guselnikov, S;Volkova, O;Baranov, K;Taranin, A;Mechetina, L; | B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans | Cytometry. Part B, Clinical cytometry | 29236355 | For this purpose, pDC cDNA from the BDCA4+ (CD304+) pDCs isolated from the peripheral blood of a healthy donor (cat. no. cDNA-PB006) was purchased from AllCells, LLC (Alameda, CA, USA). Human PBMC and spleen total RNA was isolated using Qiagen RNAeas | Full Paper |
2018 | Ikutani, M;Ogawa, S;Yanagibashi, T;Nagai, T;Okada, K;Furuichi, Y;Takatsu, K; | Elimination of eosinophils using anti-IL-5 receptor alpha antibodies effectively suppresses IL-33-mediated pulmonary arterial hypertrophy | Immunobiology | 29269115 | Human PBMCs (AllCells, Alameda, CA, USA) and Y16 cells were used as effector cells and target cells, respectively. Target cells (2 × 104 cells) were incubated with the effector cells (5 × 105 cells) and chimeric antibodies in a 96-well U-form plate a | Full Paper |
2018 | Sun, K;Mikule, K;Wang, Z;Poon, G;Vaidyanathan, A;Smith, G;Zhang, ZY;Hanke, J;Ramaswamy, S;Wang, J; | A comparative pharmacokinetic study of PARP inhibitors demonstrates favorable properties for niraparib efficacy in preclinical tumor models | Oncotarget | 30647846 | m to assess human megakaryocyte-specific development In vitro human megakaryocyte differentiation was assessed using a liquid culture system in which the human bone marrow CD34+ cells (lot # 0150114, ReachBio) derived from normal bone marrow were use | Full Paper |
2018 | Liao, C; | Regulation of Stress Erythropoiesis and the Niche: A Noval Role of Selenoproteins | Thesis | Transferrin (200µg/mL), Scf (50ng/mL), Bmp4 (15ng/mL), Gdf15 (30ng/mL), and Shh (25ng/mL), for 5 to 7 days to enrich for SEPs. Human BM cells from three subjects were purchased from Reachbio Research Labs. Human BM culture was the same condition with | Full Paper | |
2018 | Wu, H;He, L;Shi, J;Hou, X;Zhang, H;Zhang, X;An, Q;Fan, F; | Resveratrol inhibits VEGF-induced angiogenesis in human endothelial cells associated with suppression of aerobic glycolysis via modulation of PKM2 nuclear translocation | Clinical and experimental pharmacology & physiology | 30044005 | medium (DMEM) (Hyclone, Pittsburgh, PA, USA) supplemented with 10% foetal bovine serum (FBS; Cellmax, Beijing, China), 100 U/mL penicillin and 100 μg/mL streptomycin, and grown in a 95% air and 5% CO2 humidified atmosphere at 37°C, Primary HUVECs wer | Full Paper |
2018 | Ni, R;Luo, L; | A noncanonical function of histidyl-tRNA synthetase: inhibition of vascular hyperbranching during zebrafish development | FEBS open bio | 29744287 | loheximide (CHX; Sigma) from 36 hpf to observation, or treated with DMSO at the same concentration as control. Immunofluorescence staining and imaging Human umbilical vein endothelial cells (HUVECs, ALLCELLS) were cultured according to standard prot | Full Paper |
2018 | Han, XB;Tan, Y;Fang, YQ;Li, F; | Protective effects of celastrol against γ irradiation-induced oxidative stress in human umbilical vein endothelial cells | Experimental and therapeutic medicine | 30116323 | anghai Xingya Purify Material Ltd. (Shanghai, China). Western blotting detection ECL Reagent was purchased from GE Healthcare China Co. Ltd. (Beijing, China). Cell culture HUVECs were purchased from AllCells Biotech Ltd. (Shanghai, China), and grown | Full Paper |
2018 | Varma, DM;DiNicolas, MS;Nicoll, SB; | Injectable, redox-polymerized carboxymethylcellulose hydrogels promote nucleus pulposus-like extracellular matrix elaboration by human MSCs in a cell density-dependent manner | Journal of biomaterials applications | 30326804 | hMSCs were isolated from adult human bone marrow (AllCells, Emeryville, CA) via enriched density gradient centrifugation as previously described. Briefly, a hMSC Enrichment Save | Full Paper |
2018 | Zhao, X;Gao, J;Hu, X;Guo, H;Wang, F;Qiao, Y;Wang, L; | Collagen/Polyethylene Oxide Nanofibrous Membranes with Improved Hemostasis and Cytocompatibility for Wound Dressing | Applied Sciences | Human umbilical vein endothelial cells (HUVEC001) were supplied by Allcells Company (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, Shanghai, China) and 1% penicillin-streptomycin ( | Full Paper | |
2018 | Thomson-Luque, R;Wang, C;Ntumngia, FB;Xu, S;Szekeres, K;Conway, A;Adapa, SR;Barnes, SJ;Adams, JH;Jiang, RHY; | In-depth phenotypic characterization of reticulocyte maturation using mass cytometry | Blood cells, molecules & diseases | 30007855 | ). Reticulocytes and mature RBCs were kept at 4 °C in MIM until used, always before 2 days post isolation. One bone marrow reticulocyte preparation was obtained from unprocessed whole aspirates (ALLCELLS) following the same protocol as for peripheral | Full Paper |
2018 | Wang, J;Xu, J;Liu, J;Zhu, H;Peng, Y;Ding, ZM;Hua, H; | Genetic Variant Q63R of Cannabinoid Receptor 2 Causes Differential ERK Phosphorylation in Human Immune Cells | Genetic testing and molecular biomarkers | 29694791 | All human PBMCs were from commercial sources (provided by Allcells or Zenbio). Human PBMCs were thawed and cultured in the culture medium (RPMI-1640 containing 2 mM L- Save | Full Paper |
2018 | Gao, X;Yourick, JJ;Sprando, RL; | Generation of nine induced pluripotent stem cell lines as an ethnic diversity panel | Stem cell research | 30099336 | Whole blood collection from healthy donors was conducted by AllCells with informed consent under the Protocol #7000-SOP-049 Version 2.0 approved by Alpha Independent Review Save | Full Paper |
2018 | Pan, H;Zhang, M; | Serum of coronary atherosclerotic heart disease patients induces oxidative stress injury on endothelial cells | Pteridines | HUVECs were purchased from AllCells (AllCells Technology, Shanghai, China). Cells were grown in complete medium (HUVEC-004, AllCells) in an incubator with 5% CO2 at 37 °C. HUVECs were treated with the serum from patients with CAD for 24 h. HUVECs wer | Full Paper | |
2018 | Enezei, H;Ahmad, A;Takeuchi, K;Suzuki, J;Khamis, M;Razak, N;Rahman, R;Qabbani, A;Abdulhameed, E;Sugita, Y;Maeda, H;Alam, M; | Osteoinductive Activity of Bone Scaffold Bioceramic Companied with Control Release of VEGF Protein Treated Dental stem cells as A New Concept for Bone Regeneration: Part II | Journal of Hard Tissue Biology | Dental Stem Cells (DSCs) AllCells, LLC Company, Emeryville, CA, USA Dulbecco’s modified Eagles medium (DMEM) high glucose(4.5g/L) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Fetal bovine serum (FBS) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, | Full Paper | |
2018 | Sasaki, E;Momose, H;Hiradate, Y;Furuhata, K;Mizukami, T;Hamaguchi, I; | Development of a preclinical humanized mouse model to evaluate acute toxicity of an influenza vaccine | Oncotarget | 29899819 | Human PBMCs from one healthy donor (24 years old, male adult Caucasian) were obtained from AllCells (Alameda, CA, USA) and were subjected to the experiment shown in Figures 1 Save | Full Paper |
2018 | Gołąb, K;Grose, R;Placencia, V;Wickrema, A;Solomina, J;Tibudan, M;Konsur, E;Ciepły, K;Marek-Trzonkowska, N;Trzonkowski, P;Millis, JM;Fung, J;Witkowski, P; | Cell banking for regulatory T cell-based therapy: strategies to overcome the impact of cryopreservation on the Treg viability and phenotype | Oncotarget | 29515766 | We obtained de-identified human cell leukapheresis product for all the experiments from the commercial vendor AllCells, Alameda, CA, USA, therefore the study was exempt from the Save | Full Paper |
2018 | Shehadat, SA;Gorduysus, MO;Hamid, SSA;Abdullah, NA;Samsudin, AR;Ahmad, A; | Optimization of scanning electron microscope technique for amniotic membrane investigation: A preliminary study | European journal of dentistry | 30369805 | que by a cell scraper (TPP, Europe/Switzerland) was applied when necessary to remove the epithelial cells that could not be washed away by phosphate-buffered saline (PBS). Deciduous teeth stem cells (AllCells, USA) were seeded on the de-epithelialize | Full Paper |
2018 | Subhi, H;Reza, F;Husein, A;Al Shehadat, SA;Nurul, AA; | Gypsum-Based Material for Dental Pulp Capping: Effect of Chitosan and BMP-2 on Physical, Mechanical, and Cellular Properties | International journal of biomaterials | 30147725 | SHED were purchased from AllCells, USA. The cells at passage 8 were cultured in Alpha minimum essential medium [????-MEM; Invitrogen, USA (GIBCO)] supplemented with 10% fetal Save | Full Paper |
2018 | Ross, CL;Pettenati, MJ;Procita, J;Cathey, L;George, SK;Almeida-Porada, G; | Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal Cells | Global advances in health and medicine | 29796339 | me stability were not affected or altered by ELF-EMF, when used at the tested field strength and frequency. Materials and Methods Cell Culture Human bone mononuclear cells (BMNCs) were obtained from AllCells (Alameda, CA). BMNCs were enriched for th | Full Paper |
2018 | Subhi, H;Reza, F;Husein, A;Nurul, AA; | Cytotoxicity of gypsum-based biomaterial for direct pulp capping using stem cells from human exfoliated deciduous teeth | Journal of conservative dentistry : JCD | 29628642 | epare the biomaterial. The compositions of the Gyp-CHT biomaterial are shown in Table 1. Table 1 The compositions of the experimental biomaterial Cell culture preparation SHED were purchased from AllCells, USA. The cells at passage eight were cul | Full Paper |
2018 | Luddin, N;Ahmed, H;Kannan, T;Mokhtar, K;Ahmad, A; | Calcium chloride dihydrate affects the biological properties of white mineral trioxide aggregate on dental pulp stem cells: An in vitro study | Saudi Endodontic Journal | molars (adult) and characterized were purchased from AllCells (CA, USA). The complete (AllCells), with 50 ml of human mesenchymal stem cell stimulatory supplements (AllCells). Save | Full Paper | |
2018 | Goncalves, K;Hoban, M;Proctor, J;Hyzy, S;Adams, H;Gardner, J;Boitano, A;Cooke, M; | Comparison of hematopoietic stem cell expansion methods reveals that aryl hydrocarbon receptor inhibition optimally expands CD90 engrafting cells | SSRN Electronic Journal | Human cord blood CD34+ cells or mobilized peripheral blood CD34+ cells (male or female) were purchased from AllCells. Cell number, purity, and viability was confirmed at time of thaw. Cell counts were obtained using TruCount Tubes (BD). Cell viabilit | Full Paper | |
2018 | laura, ;Danilevsky, M;Li, Y;Reiss, F;Zhu, H; | SystemT: Declarative Text Understanding for Enterprise | Proceedings of the 2018 Conference of the North American Chapter of the Association for Computational Linguistics: Human Language Technologies, Volume 3 (Industry Papers) | able in AQL, including dictionary matching (AssetClassSuffixes), core NLP operators such as Semantic Role Labeling (SRL) (Verbs, Arguments), and document structure operators such as table structure understanding (AllCells). Sec. 3 details the data mo | Full Paper | |
2018 | Abhyankar, V; | The chemokine and cytokine responses of a keratinocyte, dendritic cell, and T-cell co-culture model treated with P. gingivalis hemagglutinin B (HagB) | University of Iowa | Background: P. gingivalis, a non-motile, rod-shaped, anaerobic, Gram-negative bacterium is one of the principal sources of periodontal disease. It possesses a number of potential Save | Full Paper | |
2018 | Sabhachandani, ; | Development of microfluidic microanalysis systems for cancer therapeutic screening | Northeastern University | cells were purchased from AllCells (#PB009-3F, AllCells, Alameda, CA). Cells were maintained in RPMI-1640 medium supplemented with 10% Fetal Bovine Serum and 1% Antibiotic-Antimycotic solution (Corning Cellgro, Manassas, VA). Primary human CD56+ NK c | Full Paper | |
2018 | Bates, A; | Matrix metalloproteinase and cytokine profiles from cell co-cultures and their role in oral inflammation and head and neck cancer | University of Iowa | Human monocyte-derived immature myeloid dendritic cells (DC, AllCells, Alameda, CA) were positively identified by flow cytometry and contained DC of myeloid origin: 31.5% CD1c+/79.1% CD11c+ cells and 54.4% CD141+ /79.1% CD11c+ cells (Human myeloid DC | Full Paper | |
2018 | Nada, M; | Adoptive cancer immunotherapy with human Vγ2vδ2 T cells | University of Iowa | Ex vivo expansion of Vγ2Vδ2 T cells PBMC from random healthy adult donors were obtained by density centrifugation over Ficoll-Hypaque (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) of either random donor leukopaks (12 donors) (obtained from AllCe | Full Paper | |
2018 | Twyman, RM;Wisden, W; | Development, Molecular Aspects | Advance Molecular Biology | A fate map is a map of an egg or embryo which shows the fates of allcells A cell which will form gametes. Germ cells usually segregate from somatic cells early in animal development Save | Full Paper | |
2018 | Riepe, C;Zelin, E;Wyman, S;Nguyen, D;Liang, J;Frankino, P;Meacham, Z;Vu, J;Marson, A;Ingolia, N;Corn, J; | Double Stranded DNA Breaks and Genome Editing Trigger Ribosome Remodeling and Translational Shutdown | bioRxiv | Mobilized Peripheral Blood CD34+ Stem/Progenitor Cells (AllCells) were cultured in StemSpan™ Serum Free Expansion Media II (STEMCELL Technologies), StemSpan™ StemSpan(tm) CC110 (STEMCELL Technologies), 1% Pen/Strep | Full Paper | |
2018 | Mimitou, E;Cheng, A;Montalbano, A;Hao, S;Stoeckius, M;Legut, M;Roush, T;Herrera, A;Papalexi, E;Ouyang, Z;Satija, R;Sanjana, N;Koralov, S;Smibert, P; | Expanding the CITE-seq tool-kit: Detection of proteins, transcriptomes, clonotypes and CRISPR perturbations with multiplexing, in a single assay | bioRxiv | DMSO and aliquots of 1-1.5 million cells were thawed on the day of the experiment. Peripheral blood mononuclear cells (PBMCs) were obtained cryopreserved from AllCells (USA) and used immediately after thawing. NIH-3T3 (mouse) cells expressing non-tar | Full Paper | |
2018 | Setty, M;Kiseliovas, V;Levine, J;Gayoso, A;Mazutis, L;Pe’er, D; | Palantir characterizes cell fate continuities in human hematopoiesis | bioRxiv | Cryopreserved bone marrow stem/progenitor CD34+ cells from healthy donors were purchased from AllCells, LLC. (Cat. No. ABM022F) and stored in vapor phase nitrogen until use. Typical for scRNA-seq, a vial was removed from the storage and immediately t | Full Paper | |
2018 | Shin, J;Wyman, S;Dewitt, M;Bray, N;Vu, J;Corn, J; | Controlled cycling and quiescence enables homology directed repair in engraftment-enriched adult hematopoietic stem and progenitor cells | bioRxiv | Cryopreserved wildtype human mobilized peripheral blood CD34+ HSPCs from multiple volunteer donors including male and female whose age ranged from 20-35 were purchased from Allcells, Inc | Full Paper | |
2018 | Wong, D; | Immuno-Chemotherapeutic Platinum(IV) Prodrugs of Cisplatin as Multimodal Anticancer Agents | Rethinking Platinum Anticancer Drug Design: Towards Targeted and Immuno-chemotherapeutic Approaches | Prior to activation, frozen PBMCs (AllCells LLC, USA) were thawed and washed twice with complete RPMI to remove freezing medium. 4.1 × 10 6 PBMCs were incubated in 5 mL of Save | Full Paper | |
2018 | Johnsen, KH; | Performance Analysis and Optimization of the Equelle DSL | Thesis | Gradient() takes a CollOfScalar as argument which is defined on AllCells and returns a CollOfScalar which is defined on InteriorFaces. The Divergence() function is used to calculate the Save | Full Paper | |
2018 | Ganaie, SS; | Essential Host Factors for Human Parvovirus B19 Replication | Thesis | We purchased CD34+ hematopoietic stem cells, which were isolated from bone marrow of a healthy human donor, from AllCells LLC (Alameda, CA) without any identification information on the cells, and, therefore, an institutional review board (IRB) revie | Full Paper | |
2018 | Fink, EC; | Investigating the Mechanism and Species-Specificity of Thalidomide Derivatives | Thesis | Control cord-blood CD34+ cells were isolated as above. Adult bone marrow CD34+ cells were purchased as single-donor lots from AllCells (Alameda, CA). The number of replicates for each patient sample, vector, and treatment was limited by the number of | Full Paper | |
2018 | Ahmed, MA; | Exploring the impact of hypoxia mimetic agents on multipotent stem cell biology | Thesis | Fresh human bone marrow aspirate ABM001-1 ALLCELLS Gel Loading Buffer (0.05% bromophenol blue, 40% sucrose, 0.1 M EDTA, 0.5% SDS) G2526 Sigma-Aldrich Glycine 50046 SIGMA-ALDRICH Horse serum H 1270 Sigma-Aldrich GAP-43 Antibody NB300-143 Novus Biologi | Full Paper | |
2018 | Sebastian Kettinger, N; | Investigating the HIV Reservoir in Hematopoietic Stem and Progenitor Cells | Thesis | (eBioscience), and 7-Aminoactinomycin D (7-AAD). Healthy donor BMMCs (AllCells) were stained with R-phycoerythrin-conjugated anti-CD133, R-phycoerythrin-Cy7-conjugated anti-CD34 (BD), and 7-AAD. Samples were analyzed on a BD FACSCan | Full Paper | |
2018 | Al-Jumaily, RMK; | Molecular cytology of hypoxic cancer and stem cells: an epigenetic approach | Thesis | 1 BMA-16 Human Bone Marrow, Male, Age-29 years Lonza, MD, USA 2 BMA-20 Human Bone Marrow, Female, Age-50 years AllCells, LLC, SA 3 BMA-25 Human Bone Marrow, Male, Age-27 years Lonza, MD, USA | Full Paper | |
2018 | Becker, AH; | Epigenetic and Gene Expression Changes Mediated by Histone H3 Methylation in Acute Myeloid Leukemia | Thesis | 2.1.2 Bone marrow CD34+ cells Cryopreserved bone marrow CD34+ cells from normal donors were obtained from Allcells (Alameda, CA). 2.1.3 K562 cells Human K562 cells (ATCC CCL-243) were grown in RPMI 1640 GlutaMAX medium (Gibco, Thermo Fisher Scientifi | Full Paper | |
2018 | Bartenstein, M; | Symptomatic treatment of myelofibrosis by TGF-β1 signaling inhibition | Thesis | University of Chicago. Cells were purified immune-magnetically from granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood of healthy donors purchased from AllCells, Inc. using a paramagnetically labeled mouse anti-human CD34 antibo | Full Paper | |
2018 | Siriwon, N; | Engineered CAR-T Cells for Treatment of Solid Cancers | Thesis | Frozen human PBMCs were obtained from AllCells (Alameda, CA). PBMCs were thawed in TCM and rested overnight. Before retroviral transduction, PBMCs were activated for 2 days by culturing with Dynabeads human T-activator CD3/CD28 (Thermo Fisher Scienti | Full Paper | |
2018 | Seet, C; | In Vitro Generation of Adaptive Immunity from Hematopoietic Stem Cells | Thesis | deliveries at UCLA. Bone marrow (BM) was obtained from healthy adult donors (ages 18-51) through discarded material from allogeneic BM donor harvests at UCLA or purchased from AllCells Inc. (Alameda, CA). G-CSF mobilized peripheral blood was obtained | Full Paper | |
2018 | Lin, HA; | Development and Functional Evaluation of Injectable Cellulose-based Hydrogels as Nucleus Pulposus Replacements for Intervertebral Disc Repair | Thesis | 2.2.4. Cell-laden hydrogel fabrication and characterization 2.2.4.1. Primary cell isolation Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density gradient centrifugation as previously described (Gupta et al., 201 | Full Paper | |
2018 | Marvin, CM; | Spatial and Temporal Delivery of Small Molecules from Long Wavelength Sensitive Phototherapeutcs Using Erythrocytes as Biological Carriers | Thesis | 525 nm and 660 nm LEDs were purchased from LED Supply. 725 nm and 780 nm LEDs were purchased from Techmark. Erythrocytes were purchased from Allcells. HRMS data was acquired on a Thermo Scientific electrospray linear trap quadrupole fourier transform | Full Paper | |
2018 | Hu, C; | Data-Driven Discovery of Proteomic Hallmarks in Acute Myeloid Leukemia | Thesis | The samples were normalized to a concentration of 1x104 cells/µL and a whole cell lysate was prepared as described in publications [103, 36]. In addition to AML patient samples, each array includes the same ten cryopreserved normal bone marrow CD34+ | Full Paper | |
2018 | Sun, V; | Engineering Solutions for Biomaterials: Self-Assembly and Surface-Modification of Polymers for Clinical Applications | Thesis | Human peripheral blood mononuclear cells (PBMCs) were obtained from AllCells (Alameda, CA). Frozen PBMCs were thawed and cultured in TCM containing X-VIVO™ 15 serum-free medium (Lonza, Allendale NJ), 5% (vol/vol) GemCell human serum antibody AB (Gemi | Full Paper | |
2018 | Weng, H;Huang, H;Wu, H;Qin, X;Zhao, BS;Dong, L;Shi, H;Skibbe, J;Shen, C;Hu, C;Sheng, Y;Wang, Y;Wunderlich, M;Zhang, B;Dore, LC;Su, R;Deng, X;Ferchen, K;Li, C;Sun, M;Lu, Z;Jiang, X;Marcucci, G;Mulloy, JC;Yang, J;Qian, Z;Wei, M;He, C;Chen, J; | METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and Promotes Leukemogenesis via mRNA m6A Modification | Cell stem cell | 29290617 | were then co-transduced with different combinations of retroviruses or lentiviruses as indicated through two rounds of “spinoculation”. Cells were then plated into ColonyGEL methylcellulose medium (ReachBio, Seattle, WA) supplied with 10 ng/ml of mur | Full Paper |
2018 | Probst, K;Stermann, J;von Bomhard, I;Etich, J;Pitzler, L;Niehoff, A;Bluhm, B;Xu, HC;Lang, PA;Chmielewski, M;Abken, H;Blissenbach, B;Machova, A;Papadopoulou, N;Brachvogel, B; | Depletion of Collagen IX Alpha1 Impairs Myeloid Cell Function | Stem cells (Dayton, Ohio) | 30063808 | Flushed bone marrow samples from 2 months old mice were resuspended in Iscove's Modified Dulbecco's Medium containing 10% FCS and used for the colony forming unit assay (ColonyGEL 1203, Reachbio, Seattle) | Full Paper |
2018 | Li, G;Boucher, JC;Kotani, H;Park, K;Zhang, Y;Shrestha, B;Wang, X;Guan, L;Beatty, N;Abate-Daga, D;Davila, ML; | 4-1BB enhancement of CAR T function requires NF-κB and TRAFs | JCI insight | 30232281 | Human peripheral blood mononuclear cells from healthy donors were purchased from ReachBio. Human T cell complete medium consists of RPMI1640 medium, 10% FBS, 2 mM L- Save | Full Paper |
2018 | Kong, J;Han, L;Su, H;Hu, Y;Huang, X;Lou, Y; | Riligustilide Attenuated Renal Injury by the Blockade of Renin | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 30308499 | Mouse anti-CBP monoclonal antibody SC-7300; rabbit anti-CREB polyclonal antibody SC-186 were commercially purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA;). CY5 was obtained from ReachBio WA, USA (#111-165-003), and Alexa Fluor® 488 was | Full Paper |
2018 | Psatha, N;Reik, A;Phelps, S;Zhou, Y;Dalas, D;Yannaki, E;Levasseur, DN;Urnov, FD;Holmes, MC;Papayannopoulou, T; | Disruption of the BCL11A Erythroid Enhancer Reactivates Fetal Hemoglobin in Erythroid Cells of Patients with β-Thalassemia Major | Molecular therapy. Methods & clinical development | 30182035 | anulocyte macrophage [GM] and burst-forming unit-erythroid [BFU-E]), cells were plated at a density of 750-2,000 cells/mL in semi-solid methylcellulose-based medium containing cytokines, ColonyGEL (ReachBio). Zinc Finger Nucleases ZFN pairs were exp | Full Paper |
2018 | Li, C;Psatha, N;Gil, S;Wang, H;Papayannopoulou, T;Lieber, A; | HDAd5/35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem Cells | Molecular therapy. Methods & clinical development | 30038942 | ng/mL SCF, 3 IU/mL EPO, 10 μg/mL insulin, 330 μg/mL transferrin, 2 U/mL heparin, and 1 μg/mL DOX for 6 days. CFU Assay CFU assays with transduced CD34+ cells were performed using ColonyGEL (Reachbio, Seattle, WA, USA) with human complete medium acco | Full Paper |
2018 | Wang, H;Richter, M;Psatha, N;Li, C;Kim, J;Liu, J;Ehrhardt, A;Nilsson, SK;Cao, B;Palmer, D;Ng, P;Izsvák, Z;Haworth, KG;Kiem, HP;Papayannopoulou, T;Lieber, A; | A Combined In Vivo HSC Transduction/Selection Approach Results in Efficient and Stable Gene Expression in Peripheral Blood Cells in Mice | Molecular therapy. Methods & clinical development | 29255741 | Biotec, San Diego, CA, USA) was used in accordance with the manufacturer’s instructions. CFU Assay 1,500 lineage−, GFP+ cells were plated in triplicates in ColonyGEL 1202 mouse complete medium (ReachBio, Seattle, WA, USA) and incubated for 12 days a | Full Paper |
2017 | Lim, JS;Ibaseta, A;Fischer, MM;Cancilla, B;O'Young, G;Cristea, S;Luca, VC;Yang, D;Jahchan, NS;Hamard, C;Antoine, M;Wislez, M;Kong, C;Cain, J;Liu, YW;Kapoun, AM;Garcia, KC;Hoey, T;Murriel, CL;Sage, J; | Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancer | Nature | 28489825 | ... Luminex assay for midkine protein in human plasma Human plasma samples from cancer-free normal donors were purchased from BioreclamationIVT. SCLC donor plasma was sourced from Conversant Biologics (Conversant Bio). The samples were collected, p | Full Paper |
2017 | Alley, EW;Lopez, J;Santoro, A;Morosky, A;Saraf, S;Piperdi, B;van Brummelen, E; | Clinical safety and activity of pembrolizumab in patients with malignant pleural mesothelioma (KEYNOTE-028): preliminary results from a non-randomised, open-label, phase 1b trial | Lancet Oncol. | 28291584 | PD-L1 expression was assessed in archival or new tumour samples by a central laboratory (QualTek Molecular Laboratories, Goleta, CA, USA) with a prototype assay and the 22C3 antibody (Merck, Kenilworth, NJ, USA).22, 23 PD-L1 positivity was defined by | Full Paper |
2017 | Plimack, ER;Bellmunt, J;Gupta, S;Berger, R;Chow, LQ;Juco, J;Lunceford, J;Saraf, S;Perini, RF;O'Donnell, PH; | Safety and activity of pembrolizumab in patients with locally advanced or metastatic urothelial cancer (KEYNOTE-012): a non-randomised, open-label, phase 1b study | Lancet Oncol. | 28081914 | Initially, we used a prototype PD-L1 immunohistochemical assay to establish the eligibility of patients in KEYNOTE-012, which was done at a laboratory site at QualTek (Goleta, CA, USA) accredited by the College of American Pathologists and Clinical L | Full Paper |
2017 | Frenel, JS;Le Tourneau, C;O'Neil, B;Ott, PA;Piha-Paul, SA;Gomez-Roca, C;van Brummelen, EMJ;Rugo, HS;Thomas, S;Saraf, S;Rangwala, R;Varga, A; | Safety and Efficacy of Pembrolizumab in Advanced, Programmed Death Ligand 1-Positive Cervical Cancer: Results From the Phase Ib KEYNOTE-028 Trial | J. Clin. Oncol. | 29095678 | tumor sample or newly obtained core or excisional biopsy sample, was defined as membranous staining on $ 1% modified proportion score or interface pattern as assessed using a laboratory-developed prototype immunohistochemistry assay (QualTek Molecula | Full Paper |
2017 | Hsu, C;Lee, SH;Ejadi, S;Even, C;Cohen, RB;Le Tourneau, C;Mehnert, JM;Algazi, A;van Brummelen, EMJ;Saraf, S;Thanigaimani, P;Cheng, JD;Hansen, AR; | Safety and Antitumor Activity of Pembrolizumab in Patients With Programmed Death-Ligand 1-Positive Nasopharyngeal Carcinoma: Results of the KEYNOTE-028 Study | J. Clin. Oncol. | 28837405 | Tumor PD-L1 positivity was established at a central laboratory at baseline on an archived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy sample using a laboratory-developed prototype immunohistochemical assay (QualTek Molec | Full Paper |
2017 | Ott, PA;Elez, E;Hiret, S;Kim, DW;Morosky, A;Saraf, S;Piperdi, B;Mehnert, JM; | Pembrolizumab in Patients With Extensive-Stage Small-Cell Lung Cancer: Results From the Phase Ib KEYNOTE-028 Study | J. Clin. Oncol. | 28813164 | Tumor PD-L1 expression was required to be eligible for treatment in the study; it was evaluated in archival or fresh tumor samples by a central laboratory (QualTek Molecular Laboratories, Goleta, CA) by using a prototype assay and the 22C3 antibody ( | Full Paper |
2017 | Chen, R;Zinzani, PL;Fanale, MA;Armand, P;Johnson, NA;Brice, P;Radford, J;Ribrag, V;Molin, D;Vassilakopoulos, TP;Tomita, A;von Tresckow, B;Shipp, MA;Zhang, Y;Ricart, AD;Balakumaran, A;Moskowitz, CH;, ; | Phase II Study of the Efficacy and Safety of Pembrolizumab for Relapsed/Refractory Classic Hodgkin Lymphoma | J. Clin. Oncol. | 28441111 | PD-L1 expression was determined as previously described, 16 using fresh or archival formalin-fixed, paraffin-embedded pretreatment tissue sectioned at 4 to 5 microns, with a proprietary immunohistochemical assay developed at QualTek Molecular Laborat | Full Paper |
2017 | Colombo, I;Lien, S;Yang, C;Clouthier, D;Bonilla, L;Cyriac, S;Ethier, J;Lee, Y;Kanjanapan, Y;Mandilaras, V;Dhani, N;Butler, M;Oza, A;Quintos, J;Chow, H;Pugh, T;Ohashi, P;Siu, L;Lheureux, S; | Immunologic and genomic characterization of high grade serous ovarian cancer (HGSOC) in patients (pts) treated with pembrolizumab (Pembro) in the phase II INSPIRE trial. | JCO | Mean Tumor Proportion Score of PD-L1 by IHC (Qualtek) was 6.4% (range 0-30%). Grade 3/4 adverse events possibly related to Pembro were observed in 4/18 (22%) pts; none was fatal and the most common were fatigue and hyponatremia | Full Paper | |
2017 | Toy, W;Weir, H;Razavi, P;Lawson, M;Goeppert, AU;Mazzola, AM;Smith, A;Wilson, J;Morrow, C;Wong, WL;De Stanchina, E;Carlson, KE;Martin, TS;Uddin, S;Li, Z;Fanning, S;Katzenellenbogen, JA;Greene, G;Baselga, J;Chandarlapaty, S; | Activating ESR1 Mutations Differentially Affect the Efficacy of ER Antagonists | Cancer Discov | 27986707 | ... n). PATIENT-DERIVED XENOGRAFTS (PDX) CTC-714 PDX model was derived from patient circulating tumor cells (CTC) cultures, consented according to the Human Biological Samples Policy and purchased from Conversant Biologics. The CTCs were obtained f | Full Paper |
2017 | Li, J;Stagg, NJ;Johnston, J;Harris, MJ;Menzies, SA;DiCara, D;Clark, V;Hristopoulos, M;Cook, R;Slaga, D;Nakamura, R;McCarty, L;Sukumaran, S;Luis, E;Ye, Z;Wu, TD;Sumiyoshi, T;Danilenko, D;Lee, GY;Totpal, K;Ellerman, D;Hötzel, I;James, JR;Junttila, TT; | Membrane-Proximal Epitope Facilitates Efficient T Cell Synapse Formation by Anti-FcRH5/CD3 and Is a Requirement for Myeloma Cell Killing | Cancer Cell | 28262555 | ... ec). CD4+ or CD8+ cells were used as effectors in a 3:1 effector:target ratio. In Vitro Cytotoxicity Assay: Human Plasma Cells and Primary MM Samples Human BMMCs from MM patients were procured from Conversant Bio. Human bone marrow aspirates of | Full Paper |
2017 | Le Pichon, CE;Meilandt, WJ;Dominguez, S;Solanoy, H;Lin, H;Ngu, H;Gogineni, A;Sengupta Ghosh, A;Jiang, Z;Lee, SH;Maloney, J;Gandham, VD;Pozniak, CD;Wang, B;Lee, S;Siu, M;Patel, S;Modrusan, Z;Liu, X;Rudhard, Y;Baca, M;Gustafson, A;Kaminker, J;Carano, RAD;Huang, EJ;Foreman, O;Weimer, R;Scearce-Levie, K;Lewcock, JW; | Loss of dual leucine zipper kinase signaling is protective in animal models of neurodegenerative disease | Sci Transl Med | 28814543 | AD (early-stage AD) or confirmed AD along with age-matched controls from Folio Biosciences or Banner Sun Health Research Institute. All human tissues were collected with informed consents and institutional review board approvals. | Full Paper |
2017 | Pearlman, R;Frankel, WL;Swanson, B;Zhao, W;Yilmaz, A;Miller, K;Bacher, J;Bigley, C;Nelsen, L;Goodfellow, PJ;Goldberg, RM;Paskett, E;Shields, PG;Freudenheim, JL;Stanich, PP;Lattimer, I;Arnold, M;Liyanarachchi, S;Kalady, M;Heald, B;Greenwood, C;Paquette, I;Prues, M;Draper, DJ;Lindeman, C;Kuebler, JP;Reynolds, K;Brell, JM;Shaper, AA;Mahesh, S;Buie, N;Weeman, K;Shine, K;Haut, M;Edwards, J;Bastola, S;Wickham, K;Khanduja, KS;Zacks, R;Pritchard, CC;Shirts, BH;Jacobson, A;Allen, B;de la Chapelle, A;Hampel, H;, ; | Prevalence and Spectrum of Germline Cancer Susceptibility Gene Mutations Among Patients With Early-Onset Colorectal Cancer | JAMA Oncol | 27978560 | Myriad Genetics Inc donated the next-generation sequencing testing for mismatch repair–proficient patients with CRC diagnosed younger than 50 years for this study. Dr Kalady discloses honorarium from Helomics and Transenterix and is a member of the s | Full Paper |
2017 | Ott, PA;Piha-Paul, SA;Munster, P;Pishvaian, MJ;van Brummelen, EMJ;Cohen, RB;Gomez-Roca, C;Ejadi, S;Stein, M;Chan, E;Simonelli, M;Morosky, A;Saraf, S;Emancipator, K;Koshiji, M;Bennouna, J; | Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with recurrent carcinoma of the anal canal | Ann. Oncol. | 28453692 | mbedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek Molecular Laboratories, Goleta, CA) [15] u | Full Paper |
2017 | Hui, R;Garon, EB;Goldman, JW;Leighl, NB;Hellmann, MD;Patnaik, A;Gandhi, L;Eder, JP;Ahn, MJ;Horn, L;Felip, E;Carcereny, E;Rangwala, R;Lubiniecki, GM;Zhang, J;Emancipator, K;Roach, C;Rizvi, NA; | Pembrolizumab as first-line therapy for patients with PD-L1-positive advanced non-small cell lung cancer: a phase 1 trial | Ann. Oncol. | 28168303 | As previously described [5], PD-L1 expression for enrollment was assessed with a prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA) and the relationship between PD-L1 expression and efficacy was investigated using a cli | Full Paper |
2017 | Potts, AH;Vakulskas, CA;Pannuri, A;Yakhnin, H;Babitzke, P;Romeo, T; | Global role of the bacterial post-transcriptional regulator CsrA revealed by integrated transcriptomics | Nat Commun | 29150605 | ;... nufacturer’s instructions with one modification. Adaptors and reverse transcription primers were diluted 1 in 5 in water before use. Libraries were sequenced using HiSeq 2500 50SE (Illumina) by the HudsonAlpha Genomic Services Laboratory. Seque | Full Paper |
2017 | Christopher, MA;Myrick, DA;Barwick, BG;Engstrom, AK;Porter-Stransky, KA;Boss, JM;Weinshenker, D;Levey, AI;Katz, DJ; | LSD1 protects against hippocampal and cortical neurodegeneration | Nat Commun | 28993646 | ... s possible, then RNA was precipitated with isopropanol. Pellets were then washed with 75% ethanol and resuspended in 50 µL deionized water. RNA library preparation and sequencing were performed by HudsonAlpha Genomic Services Lab. RNA was Poly(A) | Full Paper |
2017 | Ramaker, RC;Savic, D;Hardigan, AA;Newberry, K;Cooper, GM;Myers, RM;Cooper, SJ; | A genome-wide interactome of DNA-associated proteins in the human liver | Genome Res. | 29021291 | HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for Bi | Full Paper |
2017 | Cubeñas-Potts, C;Rowley, MJ;Lyu, X;Li, G;Lei, EP;Corces, VG; | Different enhancer classes in Drosophila bind distinct architectural proteins and mediate unique chromatin interactions and 3D architecture | Nucleic Acids Res. | 27899590 | New ChIP experiments were performed in Kc167 cells for GAF, H3, H3K27me3, Ibf1, Ibf2, Pita, Nup98 and ZIPIC as described previously with minor modifications (28). More precisely, antibody-bound protein complexes were isolated with Protein A or Protei | Full Paper |
2017 | Edwardson, CF;Hollibaugh, JT; | Metatranscriptomic analysis of prokaryotic communities active in sulfur and arsenic cycling in Mono Lake, California, USA | ISME J | 28548659 | synthesis kits. Libraries (~225 bp insert) were prepared using Illumina TruSeq technology. Samples were pooled and run on one lane of 150 × 2 Illumina HiSeq 2500 Rapid Run at HudsonAlpha Genomic Services. Reads have | Full Paper |
2017 | Yeh, TC;O'Connor, G;Petteruti, P;Dulak, A;Hattersley, M;Barrett, JC;Chen, H; | Identification of CCR2 and CD180 as Robust Pharmacodynamic Tumor and Blood Biomarkers for Clinical Use with BRD4/BET Inhibitors | Clin. Cancer Res. | 28073847 | Whole blood was collected in 10 mL EDTA vaccutainers (Becton Dickinson) from two control healthy volunteers and 11 patients with cancer with solid tumor or hematologic cancers through Conversant Bio. Compound incubation and sample processing was carr | Full Paper |
2017 | Kirtane, AR;Sadhukha, T;Kim, H;Khanna, V;Koniar, B;Panyam, J; | Fibrinolytic Enzyme Cotherapy Improves Tumor Perfusion and Therapeutic Efficacy of Anticancer Nanomedicine | Cancer Res. | 28108516 | ... anticancer nanomedicine in syngeneic mouse melanoma and xenograft lung cancer models. Go to: MATERIALS AND METHODS MATERIALS Tissue microarrays of various human tumor biopsies were obtained from Folio Biosciences (Ohio, USA). Anti-human fibr | Full Paper |
2017 | Jung, YH;Sauria, MEG;Lyu, X;Cheema, MS;Ausio, J;Taylor, J;Corces, VG; | Chromatin States in Mouse Sperm Correlate with Embryonic and Adult Regulatory Landscapes | Cell Rep | 28178516 | We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology, and specially Drs. Braden Boone, Angela Jones and Terri Pointer, for their help in performing Illumina sequencing of samples. This work was supported by U | Full Paper |
2017 | O'Daniel, JM;McLaughlin, HM;Amendola, LM;Bale, SJ;Berg, JS;Bick, D;Bowling, KM;Chao, EC;Chung, WK;Conlin, LK;Cooper, GM;Das, S;Deignan, JL;Dorschner, MO;Evans, JP;Ghazani, AA;Goddard, KA;Gornick, M;Farwell Hagman, KD;Hambuch, T;Hegde, M;Hindorff, LA;Holm, IA;Jarvik, GP;Knight Johnson, A;Mighion, L;Morra, M;Plon, SE;Punj, S;Richards, CS;Santani, A;Shirts, BH;Spinner, NB;Tang, S;Weck, KE;Wolf, SM;Yang, Y;Rehm, HL; | A survey of current practices for genomic sequencing test interpretation and reporting processes in US laboratories | Genet. Med. | 27811861 | Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.++Laboratory for Molecular Medicine, Partners Healthcare Personalized Medicine, Cambridge, Massachusetts, USA.++Division of Medical Genetics, Univer | Full Paper |
2017 | Rydzak, T;Garcia, D;Stevenson, DM;Sladek, M;Klingeman, DM;Holwerda, EK;Amador-Noguez, D;Brown, SD;Guss, AM; | Deletion of Type I glutamine synthetase deregulates nitrogen metabolism and increases ethanol production in Clostridium thermocellum | Metab. Eng. | 28400329 | ; diluted. Sequencing was completed using a SR50 sequencing protocol and V4 chemistry on an Illumina HiSeq. 2500 platform (HudsonAlpha Genomic Services Laboratory; Huntsville, AL). 2.7. RNA-Seq analysis. Raw reads | Full Paper |
2017 | Tigue, NJ;Bamber, L;Andrews, J;Ireland, S;Hair, J;Carter, E;Sridharan, S;Jovanović, J;Rees, DG;Springall, JS;Solier, E;Li, YM;Chodorge, M;Perez-Martinez, D;Higazi, DR;Oberst, M;Kennedy, M;Black, CM;Yan, L;Schwickart, M;Maguire, S;Cann, JA;de Haan, L;Young, LL;Vaughan, T;Wilkinson, RW;Stewart, R; | MEDI1873, a potent, stabilized hexameric agonist of human GITR with regulatory T-cell targeting potential | Oncoimmunology | 28405505 | ... xP3 Tissue samples from 76 cancer patients, with NSCLC, colorectal cancer (CRC), bladder cancer, or squamous cell cancer of the head and neck (SCCHN), were obtained from commercial sources (Asterand, ConversantBio, ProteoGenex, National Disease R | Full Paper |
2017 | Liu, Z;Zhang, C;Skamagki, M;Khodadadi-Jamayran, A;Zhang, W;Kong, D;Chang, CW;Feng, J;Han, X;Townes, TM;Li, H;Kim, K;Zhao, R; | Elevated p53 Activities Restrict Differentiation Potential of MicroRNA-Deficient Pluripotent Stem Cells | Stem Cell Reports | 29141234 | Total RNA was extracted from Dgcr8−/−-302 cells and submitted to the Genomic Services Lab at the HudsonAlpha Institute. The library was constructed by the standard miRNA library construction protocol (Illumina) and 15 million, 50 bp single-end reads | Full Paper |
2017 | Bowling, KM;Thompson, ML;Amaral, MD;Finnila, CR;Hiatt, SM;Engel, KL;Cochran, JN;Brothers, KB;East, KM;Gray, DE;Kelley, WV;Lamb, NE;Lose, EJ;Rich, CA;Simmons, S;Whittle, JS;Weaver, BT;Nesmith, AS;Myers, RM;Barsh, GS;Bebin, EM;Cooper, GM; | Genomic diagnosis for children with intellectual disability and/or developmental delay | Genome Med | 28554332 | Methods" (see Additional file 1). Whole-exome and whole-genome sequencing. Blood samples were sent for sequencing at the HudsonAlpha Genomic Services Laboratory (http://gsl.hudsonalpha.org). Genomic DNA was isolated | Full Paper |
2017 | Harden, ME;Prasad, N;Griffiths, A;Munger, K; | Modulation of microRNA-mRNA Target Pairs by Human Papillomavirus 16 Oncoproteins | MBio | 28049151 | ;... million 50-bp PE reads) was performed with the Illumina MiSeq Sequencing System (Illumina). miR-seq data analysis. Postprocessing of the miR-seq reads from each sample was performed according to the HudsonAlpha Genomic Services Laboratory (GSL) | Full Paper |
2017 | Weiss, G;Schlegel, A;Kottwitz, D;König, T;Tetzner, R; | Validation of the SHOX2/PTGER4 DNA Methylation Marker Panel for Plasma-Based Discrimination between Patients with Malignant and Nonmalignant Lung Disease | J Thorac Oncol | 27544059 | The patient samples were either material collected over the course of several months by commercial partners (ProteoGenex Inc., Sofia Bio LLC) or leftover material from previously conducted clinical trials in colon cancer.21, 22 More specifically, pat | Full Paper |
2017 | Steffen, MM;Davis, TW;McKay, RML;Bullerjahn, GS;Krausfeldt, LE;Stough, JMA;Neitzey, ML;Gilbert, NE;Boyer, GL;Johengen, TH;Gossiaux, DC;Burtner, AM;Palladino, D;Rowe, MD;Dick, GJ;Meyer, KA;Levy, S;Boone, BE;Stumpf, RP;Wynne, TT;Zimba, PV;Gutierrez, D;Wilhelm, SW; | Ecophysiological Examination of the Lake Erie Microcystis Bloom in 2014: Linkages between Biology and the Water Supply Shutdown of Toledo, OH | Environ. Sci. Technol. | 28535339 | RNA was stored at −80 °C until sent to HudsonAlpha Institute for Biotechnology (Huntsville, AL) for sequencing. Total RNA concentrations and quality were assessed fluorometrically via RiboGreen (Life Technologies, Carlsbad, CA) followed by integrity | Full Paper |
2017 | Park, KC;Lee, M;Jeon, Y;Jeon, R;Baek, SH;Lee, H;Kim, KI; | Skin-Specific Deletion of Mis18α Impedes Proliferation and Stratification of Epidermal Keratinocytes | J. Invest. Dermatol. | 27670610 | Materials and Methods. In situ hybridization. All procedures of in situ hybridization, except probe design and cloning, were performed by Phylogeny Inc. (Columbus, OH). Briefly, the whole bodies of C57BL/6 mice were frozen and cut into 10-μm sections | Full Paper |
2017 | Blanchet, P;Bebin, M;Bruet, S;Cooper, GM;Thompson, ML;Duban-Bedu, B;Gerard, B;Piton, A;Suckno, S;Deshpande, C;Clowes, V;Vogt, J;Turnpenny, P;Williamson, MP;Alembik, Y;, ;, ;Glasgow, E;McNeill, A; | MYT1L mutations cause intellectual disability and variable obesity by dysregulating gene expression and development of the neuroendocrine hypothalamus | PLoS Genet. | 28859103 | Département de Génétique Médicale, Hôpital Arnaud de Villeneuve, Montpellier Cedex 5, France.++Department of Neurology, University of Alabama at Birmingham, Birmingham, AL, United States of America.++Service de génétique médicale, Hôpitaux Universita | Full Paper |
2017 | Mojiri, A;Stoletov, K;Carrillo, MA;Willetts, L;Jain, S;Godbout, R;Jurasz, P;Sergi, CM;Eisenstat, DD;Lewis, JD;Jahroudi, N; | Functional assessment of von Willebrand factor expression by cancer cells of non-endothelial origin | Oncotarget | 28035064 | Immunofluorescent staining of cultured cells were performed as previously described [34]. Immunofluorescent staining of slides containing sectioned paraffin imbedded biopsies of normal human brain and glioma (Dr. D. Eisenstat from the Brain Tumor Tis | Full Paper |
2017 | McDaniel, JM;Varley, KE;Gertz, J;Savic, DS;Roberts, BS;Bailey, SK;Shevde, LA;Ramaker, RC;Lasseigne, BN;Kirby, MK;Newberry, KM;Partridge, EC;Jones, AL;Boone, B;Levy, SE;Oliver, PG;Sexton, KC;Grizzle, WE;Forero, A;Buchsbaum, DJ;Cooper, SJ;Myers, RM; | Genomic regulation of invasion by STAT3 in triple negative breast cancer | Oncotarget | 28030809 | ... ell viability and invasion assays. All ChIP-seq and RNA-seq datasets have been deposited in GEO (accession number: GSE85579). SUPPLEMENTARY MATERIALS FIGURES AND TABLES We thank the members of the HudsonAlpha Genomic Services Laboratory for pr | Full Paper |
2017 | Ranganathan, P;Ngankeu, A;Zitzer, NC;Leoncini, P;Yu, X;Casadei, L;Challagundla, K;Reichenbach, DK;Garman, S;Ruppert, AS;Volinia, S;Hofstetter, J;Efebera, YA;Devine, SM;Blazar, BR;Fabbri, M;Garzon, R; | Serum miR-29a Is Upregulated in Acute Graft-versus-Host Disease and Activates Dendritic Cells through TLR Binding | J. Immunol. | 28159900 | ... Go to: ACKNOWLEDGMENTS We thank Carlo M Croce at The Ohio State University for providing us with miR29a KO mice. We thank Jordan Smith for assisting in collection and processing of blood samples. Phylogeny Inc., Columbus, Ohio, performed LNA- | Full Paper |
2017 | Xu-Monette, ZY;Zhang, M;Li, J;Young, KH; | PD-1/PD-L1 Blockade: Have We Found the Key to Unleash the Antitumor Immune Response? | Front Immunol | 29255458 | 99.5%; 75.6% of patients had a response for ≥6 months Clinical activity was seen across all PD-L1 groups defined by PD-L1 intensity score, tumor-membrane staining score, and histiocyte score (QualTek IHC assay); 90.4% of patients had an intensity | Full Paper |
2017 | Zeng, Q;Tian, X;Wang, L; | Genetic adaptation of microbial populations present in high-intensity catfish production systems with therapeutic oxytetracycline treatment | Sci Rep | 29235508 | ;... DNA (~1000 ng/sample) extracted from every sediment sample collected from the treated or the control system, were pooled for sequencing. The library construction and sequencing were conducted at the HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2017 | Oliveira, ERA;Póvoa, TF;Nuovo, GJ;Allonso, D;Salomão, NG;Basílio-de-Oliveira, CA;Geraldo, LHM;Fonseca, CG;Lima, FRS;Mohana-Borges, R;Paes, MV; | Dengue fatal cases present virus-specific HMGB1 response in peripheral organs | Sci Rep | 29167501 | ... iro, Brazil 2Instituto de Criminalística, Tocantins, Brazil 30000 0001 2285 7943grid.261331.4Ohio State University Comprehensive Cancer Center, Columbus, Ohio, United States of America 4grid.430298.4Phylogeny Inc, Powell, Ohio, United States of A | Full Paper |
2017 | Zhang, Y;Saavedra, E;Tang, R;Gu, Y;Lappin, P;Trajkovic, D;Liu, SH;Smeal, T;Fantin, V;De Botton, S;Legrand, O;Delhommeau, F;Pernasetti, F;Louache, F; | Targeting primary acute myeloid leukemia with a new CXCR4 antagonist IgG1 antibody (PF-06747143) | Sci Rep | 28779088 | ... ratio and 1:50 ratios, respectively (n = 4/group). ToxiLight bioluminescent cytotoxicity assay (Lonza) was used to detect cell lysis. The AML patient primary bone marrow sample was obtained from Conversant Bio (sample ID #110032873). Mice NSG m | Full Paper |
2017 | Aumiller, V;Strobel, B;Romeike, M;Schuler, M;Stierstorfer, BE;Kreuz, S; | Comparative analysis of lysyl oxidase (like) family members in pulmonary fibrosis | Sci Rep | 28273952 | Normal and fibrotic lung tissue samples from the BLM-induced mouse model were prepared and Masson trichrome stained as described previously27. Human IPF and healthy lung tissue was purchased at Folio bioscience (Columbus, Ohio, USA) following the gui | Full Paper |
2017 | Jane, EP;Premkumar, DR;Sutera, PA;Cavaleri, JM;Pollack, IF; | Survivin inhibitor YM155 induces mitochondrial dysfunction, autophagy, DNA damage and apoptosis in Bcl-xL silenced glioma cell lines | Mol. Carcinog. | 27805285 | collected at the time of tumor resection. We also obtained primary GBM cells from Conversant Biologics (Huntsville, AL), which were cultured in DMEM/F12 (Invitrogen, Carlsbad, CA). GIBCO® Human Astrocytes, normal human cells | Full Paper |
2017 | Appleby, TC;Greenstein, AE;Hung, M;Liclican, A;Velasquez, M;Villaseñor, AG;Wang, R;Wong, MH;Liu, X;Papalia, GA;Schultz, BE;Sakowicz, R;Smith, V;Kwon, HJ; | Biochemical characterization and structure determination of a potent, selective antibody inhibitor of human MMP9 | J. Biol. Chem. | 28235803 | Ulcerative colitis tissue was obtained after surgical colectomy (Folio Biosciences). Specimens were selected based on disease severity scored by a pathologist on matched FFPE tissues. Frozen fragments of ~50 mg were lysed in 10 mM Hepes pH 7.5, 150 m | Full Paper |
2017 | Cho, JH;Sorensen, SF;Choi, YL;Feng, Y;Kim, TE;Choi, H;Georgsen, JB;Dolled-Filhart, M;Emancipator, K;Meldgaard, P;Sun, JM;Kim, HK;Choi, YS;Shim, YM;Zhou, W;Hager, H;Kim, J; | Programmed Death Ligand 1 Expression in Paired Non-Small Cell Lung Cancer Tumor Samples | Clin Lung Cancer | 28669849 | charged slides. The TechMate 500 instrument (QualTek, Goleta, CA) was used with the 22C3 antibody for automated staining. A membrane staining. All of the PD-L1 IHC assays were done at QualTek, as the central laboratory. PD | Full Paper |
2017 | Sundahl, N;De Wolf, K;Rottey, S;Decaestecker, K;De Maeseneer, D;Meireson, A;Goetghebeur, E;Fonteyne, V;Verbeke, S;De Visschere, P;Reynders, D;Van Gele, M;Brochez, L;Ost, P; | A phase I/II trial of fixed-dose stereotactic body radiotherapy with sequential or concurrent pembrolizumab in metastatic urothelial carcinoma: evaluation of safety and clinical and immunologic response | J Transl Med | 28662677 | Exploratory endpoints. Response rate according to expression of PD-L1. PD-L1 expression will be determined by immunohistochemistry in QualTek Molecular Laboratories on an archival or newly obtained tissue sample of the tumor or metastatic lesion | Full Paper |
2017 | Lagatie, O;Van Dorst, B;Stuyver, LJ; | Identification of three immunodominant motifs with atypical isotype profile scattered over the Onchocerca volvulus proteome | PLoS Negl Trop Dis | 28125577 | For determination of specificity of the peptide ELISA’s, different sets of plasma samples were obtained from Discovery Life Sciences, Inc. (Los Osos, USA), Bioreclamation IVT (Baltimore, USA), FR3 or Mayo Clinic (Jacksonville, USA). Written informed | Full Paper |
2017 | Pellegrini, KL;Patil, D;Douglas, KJS;Lee, G;Wehrmeyer, K;Torlak, M;Clark, J;Cooper, CS;Moreno, CS;Sanda, MG; | Detection of prostate cancer-specific transcripts in extracellular vesicles isolated from post-DRE urine | Prostate | 28419548 | ; targeted in this analysis). The libraries were sequenced at the HudsonAlpha Genomic Services Lab to produce 150 bases of paired‐end reads with an average of over 300 000 reads mapped per sample. The 150 base sequence | Full Paper |
2017 | Wilson, CM;Klingeman, DM;Schlachter, C;Syed, MH;Wu, CW;Guss, AM;Brown, SD; | LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313 | Appl. Environ. Microbiol. | 28003194 | ;... Samples were pooled into three groups to make a stock of 20 nM in 72 μl. A 40-μl aliquot of the three pooled libraries was sequenced using an SR50 sequencing run on an Illumina HiSeq 2500 platform (HudsonAlpha Genomic Services Laboratory, Huntsv | Full Paper |
2017 | Zhang, Y;Song, J;Day, K;Absher, D; | dCATCH-Seq: improved sequencing of large continuous genomic targets with double-hybridization | BMC Genomics | 29061109 | HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA. dabsher@hudsona | Full Paper |
2017 | Matsunaga, Y;Hwang, H;Franke, B;Williams, R;Penley, M;Qadota, H;Yi, H;Morran, LT;Lu, H;Mayans, O;Benian, GM; | Twitchin kinase inhibits muscle activity | Mol. Biol. Cell | 28428253 | Genomic DNA from unc-22(sf21) was prepared and provided to the Emory Integrated Genomics Core (EIGC) for Illumina sequencing. The Genomic Services Laboratory at the HudsonAlpha Institute for Biotechnology performed quality control and constructed sta | Full Paper |
2017 | Liu, Z;Zhang, C;Khodadadi-Jamayran, A;Dang, L;Han, X;Kim, K;Li, H;Zhao, R; | Canonical microRNAs Enable Differentiation, Protect Against DNA Damage, and Promote Cholesterol Biosynthesis in Neural Stem Cells | Stem Cells Dev. | 27762676 | For mRNA-seq and small RNA-seq analyses, total RNA samples prepared from Dgcr8+/− and Dgcr8−/− NSCs were submitted to the Genomic Services Laboratory at HudsonAlpha Institute (Huntsville, AL) for library construction and sequencing. For mRNA-seq anal | Full Paper |
2017 | Aycock, KI;Campbell, RL;Lynch, FC;Manning, KB;Craven, BA; | Computational predictions of the embolus-trapping performance of an IVC filter in patient-specific and idealized IVC geometries | Biomech Model Mechanobiol | 28656515 | Full Paper | |
2017 | George, AF;Rahman, KM;Camp, ME;Prasad, N;Bartol, FF;Bagnell, CA; | Defining age- and lactocrine-sensitive elements of the neonatal porcine uterine microRNA-mRNA interactome | Biol. Reprod. | 28203709 | Department of Animal Sciences, Endocrinology, and Animal Biosciences Program, Rutgers University, New Brunswick, New Jersey, USA.++Department of Animal Sciences, Endocrinology, and Animal Biosciences Program, Rutgers University, New Brunswick, New Je | Full Paper |
2017 | Kirby, MK;Ramaker, RC;Roberts, BS;Lasseigne, BN;Gunther, DS;Burwell, TC;Davis, NS;Gulzar, ZG;Absher, DM;Cooper, SJ;Brooks, JD;Myers, RM; | Genome-wide DNA methylation measurements in prostate tissues uncovers novel prostate cancer diagnostic biomarkers and transcription factor binding patterns | BMC Cancer | 28412973 | ;... ng characteristic RRBS Reduced representation bisulfite sequencing TCGA The cancer genome atlas TF Transcription factor TMPRSS2 Transmembrane protease, serine 2 Acknowledgements We thank the HudsonAlpha Genomic Services Lab for providin | Full Paper |
2017 | Costa-Paiva, EM;Whelan, NV;Waits, DS;Santos, SR;Schrago, CG;Halanych, KM; | Discovery and evolution of novel hemerythrin genes in annelid worms | BMC Evol. Biol. | 28330441 | Despite extensive study on hemoglobins and hemocyanins, little is known about hemerythrin (Hr) evolutionary history. Four subgroups of Hrs have been documented, including: circulating Hr (cHr), myohemerythrin (myoHr), ovohemerythrin (ovoHr), and neur | Full Paper |
2017 | Pizzi, M;Inghirami, G; | Patient-derived tumor xenografts of lymphoproliferative disorders: are they surrogates for the human disease? | Curr. Opin. Hematol. | 28594662 | Patient-derived xenograft models WuXi AppTec http://www.urolead.com Xenografts Conversant Bio http://www.conversantbio.com/pricing Xenograft models ONCOdesign http://www.oncodesign. com/en/contact-us Humanized mice HuMurine http://humurine.com | Full Paper |
2017 | Zhang, D;Thongda, W;Li, C;Zhao, H;Beck, BH;Mohammed, H;Arias, CR;Peatman, E; | More than just antibodies: Protective mechanisms of a mucosal vaccine against fish pathogen Flavobacterium columnare | Fish Shellfish Immunol. | 28989091 | ; 2000 instrument (Thermo Scientific, Wilmington, DE). RNA-seq library preparation and sequencing were carried out by the HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). Briefly, TruSeq RNA Sample Preparation | Full Paper |
2017 | Zhu, J;Fu, Q;Ao, Q;Tan, Y;Luo, Y;Jiang, H;Li, C;Gan, X; | Transcriptomic profiling analysis of tilapia (Oreochromis niloticus) following Streptococcus agalactiae challenge | Fish Shellfish Immunol. | 28111359 | ; For each timepoint, equal amount of RNA from individual fish were pooled together (15 fish) for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out in HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) | Full Paper |
2017 | McCoy, CR;Jackson, NL;Day, J;Clinton, SM; | Genetic predisposition to high anxiety- and depression-like behavior coincides with diminished DNA methylation in the adult rat amygdala | Behav. Brain Res. | 27965039 | ; Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) for next-generation sequencing using NEBNext reagents (New England Biolabs, Ipswich, MA) according to manufacturer's recommendations | Full Paper |
2017 | Stebbins, KJ;Broadhead, AR;Cabrera, G;Correa, LD;Messmer, D;Bundey, R;Baccei, C;Bravo, Y;Chen, A;Stock, NS;Prasit, P;Lorrain, DS; | In vitro and in vivo pharmacology of NXT629, a novel and selective PPARα antagonist | Eur. J. Pharmacol. | 28483457 | expression of PPARα, normal healthy or melanoma skin biopsy samples (n=2 donors each) were obtained from ConversantBio (Hunstville, Alabama) Normal (n=5) and melanoma (n=4) human skin biopsy samples were obtained from Conversant Bio and expression | Full Paper |
2017 | Pirie-Shepherd, SR;Painter, C;Whalen, P;Vizcarra, P;Roy, M;Qian, J;Franks, T;Coskran, T;Golas, J;Deng, S;Zhong, W;Tucker, E;Marrinucci, D;Gerber, HP;Powell, EL; | Detecting expression of 5T4 in CTCs and tumor samples from NSCLC patients | PLoS ONE | 28727782 | ... d from a variety of sources, including the Pfizer tumor bank and commercial vendors. All commercially sourced human biospecimens, including blood samples and matched tumor tissues, were obtained from Conversant Bio. Biospecimens distributed by Co | Full Paper |
2017 | Awasthi Mishra, N;Drögemüller, C;Jagannathan, V;Keller, I;Wüthrich, D;Bruggmann, R;Beck, J;Schütz, E;Brenig, B;Demmel, S;Moser, S;Signer-Hasler, H;Pieńkowska-Schelling, A;Schelling, C;Sande, M;Rongen, R;Rieder, S;Kelsh, RN;Mercader, N;Leeb, T; | A structural variant in the 5'-flanking region of the TWIST2 gene affects melanocyte development in belted cattle | PLoS ONE | 28658273 | Center Zurich, and the Genomic Services Lab at HudsonAlpha for performing experiments. Computationally intensive tasks were performed at the the Interfaculty Bioinformatics Unit of the University of Bern and the Vital-IT high-performance computing ce | Full Paper |
2017 | Carlson, DE;Hedin, M; | Comparative transcriptomics of Entelegyne spiders (Araneae, Entelegynae), with emphasis on molecular evolution of orphan genes | PLoS ONE | 28379977 | Next-generation sequencing technology is rapidly transforming the landscape of evolutionary biology, and has become a cost-effective and efficient means of collecting exome information for non-model organisms. Due to their taxonomic diversity, produc | Full Paper |
2017 | Howard, LM;Hoek, KL;Goll, JB;Samir, P;Galassie, A;Allos, TM;Niu, X;Gordy, LE;Creech, CB;Prasad, N;Jensen, TL;Hill, H;Levy, SE;Joyce, S;Link, AJ;Edwards, KM; | Cell-Based Systems Biology Analysis of Human AS03-Adjuvanted H5N1 Avian Influenza Vaccine Responses: A Phase I Randomized Controlled Trial | PLoS ONE | 28099485 | Vanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, United States of America.++Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashvil | Full Paper |
2017 | O'Neil, BH;Wallmark, JM;Lorente, D;Elez, E;Raimbourg, J;Gomez-Roca, C;Ejadi, S;Piha-Paul, SA;Stein, MN;Abdul Razak, AR;Dotti, K;Santoro, A;Cohen, RB;Gould, M;Saraf, S;Stein, K;Han, SW; | Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with advanced colorectal carcinoma | PLoS ONE | 29284010 | mbedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek Molecular Laboratories, Goleta, CA, USA) [ | Full Paper |
2017 | Danaee, H;Kalebic, T;Wyant, T;Fassan, M;Mescoli, C;Gao, F;Trepicchio, WL;Rugge, M; | Consistent expression of guanylyl cyclase-C in primary and metastatic gastrointestinal cancers | PLoS ONE | 29261789 | GCC IHC analysis and pathology assessment. GCC staining was performed on the Techmate Automated IHC Platform (BioTek Solutions/Ventana Medical Systems, Tucson, AZ, USA; QualTek Laboratories, Goleta, CA, USA) ;two independent pathologists. GCC I | Full Paper |
2017 | O'Neil, BH;Wallmark, JM;Lorente, D;Elez, E; | Safety and antitumor activity of the anti–PD-1 antibody pembrolizumab in patients with advanced colorectal carcinoma | PloS one | archived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory- developed prototype immunohistochemistry (IHC) assay (QualTek Molecu | Full Paper | |
2017 | Eboigbodin, KE;Moilanen, K;Elf, S;Hoser, M; | Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA® | BMC Infect. Dis. | 28183291 | isothermal conditions. Methods. Clinical specimens and rapid processing. A total of 40 nasopharyngeal (NP) swab specimens were obtained from Discovery Life Sciences Biobank (Discovery Life Sciences, Inc., CA, USA). The | Full Paper |
2017 | Liu, DT;Brewer, MS;Chen, S;Hong, W;Zhu, Y; | Transcriptomic signatures for ovulation in vertebrates | Gen. Comp. Endocrinol. | 28111234 | PCR (qPCR) assay. 2.4. Library construction and Illumina sequencing. RNA-Seq library preparation and high-throughput NGS sequencing were carried out at HudsonAlpha Genomic Services Lab (Huntsville, AL). Qubit® 3.0 | Full Paper |
2017 | Bhayana, S;Song, F;Jacob, J;Fadda, P;Denko, NC;Xu-Welliver, M;Chakravarti, A;Jacob, NK; | Urinary miRNAs as Biomarkers for Noninvasive Evaluation of Radiation-Induced Renal Tubular Injury | Radiat. Res. | 28977780 | W81XWH-15-2-0054). We thank Dr. Jerry Nuovo of Phylogeny, Inc. (Powell, OH) for technical assistance. REFERENCES. 1. Hingorani S. Chronic kidney disease in long-term survivors of hematopoietic cell transplantation: epidemiology, pathogenesis, and tre | Full Paper |
2017 | Kadić, E;Moniz, RJ;Huo, Y;Chi, A;Kariv, I; | Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determinated by multiparametric single cell mass cytometry analysis | BMC Immunol. | 28148223 | ... ry detection antibodies Sample sourcing PBMCs were purchased from SeraCare Life Sciences Inc., (Milford, MA). Frozen dissociated tumor cells (DTC) and normal adjacent tissue (NAT) were purchased from ConversantBio (Huntsville, AL). The frozen sam | Full Paper |
2017 | Parlato, M;Molenda, J;Murphy, WL; | Specific recruitment of circulating angiogenic cells using biomaterials as filters | Acta biomaterialia | 28373084 | CD133+ cells collected from mobilized peripheral blood were purchased from AllCells, LLC. These cells were collected by AllCells, LLC from one donor (26 year old, African American, Save | Full Paper |
2017 | Block, TS;Murphy, TI;Munster, PN;Nguyen, DP;Lynch, FJ; | Glucocorticoid receptor expression in 20 solid tumor types using immunohistochemistry assay | Cancer Manag Res | 28293120 | Tiffany I Murphy. 3 QualTek Molecular Laboratories, Newtown, PA. Find articles by Tiffany I Murphy. Pamela N Munster Dat P Nguyen. 1 Corcept Therapeutics, Inc, Menlo Park, CA. Find articles by Dat P Nguyen. Frank J Lynch. 3 QualTek Molecular Labora | Full Paper |
2017 | Coletti, M;Hultquist, C;Kennedy, WG;Cervone, G; | Validating Safecast data by comparisons to a U. S. Department of Energy Fukushima Prefecture aerial survey | J Environ Radioact | 28167372 | Second, units are randomly selected, assembled, and undergo calibration tests at the Julich Research Centre in Germany, QualTek in the US, and the International Atomic Energy Agency (IAEA) testing laboratory in Seibersdorf, Austria | Full Paper |
2017 | Thabet, AA;Maas, AE;Saber, SA;Tarrant, AM; | Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposure | Mar Genomics | 28385518 | Total RNA was extracted from pools of C. limacina juveniles using the Aurum Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad). RNA purity and integrity were quantified using a Nanodrop ND-1000 spectrophotometer, and quality was assessed using a Bioana | Full Paper |
2017 | Matsushita, M;Ozawa, K;Suzuki, T;Nakamura, M;Nakano, N;Kanchi, S;Ichikawa, D;Matsuki, E;Sakurai, M;Karigane, D;Kasahara, H;Tsukamoto, N;Shimizu, T;Mori, T;Nakajima, H;Okamoto, S;Kawakami, Y;Hattori, Y; | CXorf48 is a potential therapeutic target for achieving treatment-free remission in CML patients | Blood cancer journal | 28862699 | ty gradient centrifugation and cryopreserved in Cell Banker reagent (Juji-Field, Tokyo, Japan) in liquid nitrogen until further use. Bone marrow mononuclear cells (BMMNC) from HDs were purchased from AllCells, LLC. (Alameda, CA, USA). This study was | Full Paper |
2017 | Simeonov, DR;Gowen, BG;Boontanrart, M;Roth, TL;Gagnon, JD;Mumbach, MR;Satpathy, AT;Lee, Y;Bray, NL;Chan, AY;Lituiev, DS;Nguyen, ML;Gate, RE;Subramaniam, M;Li, Z;Woo, JM;Mitros, T;Ray, GJ;Curie, GL;Naddaf, N;Chu, JS;Ma, H;Boyer, E;Van Gool, F;Huang, H;Liu, R;Tobin, VR;Schumann, K;Daly, MJ;Farh, KK;Ansel, KM;Ye, CJ;Greenleaf, WJ;Anderson, MS;Bluestone, JA;Chang, HY;Corn, JE;Marson, A; | Discovery of stimulation-responsive immune enhancers with CRISPR activation | Nature | 28854172 | Normal donor human peripheral blood cells were obtained fresh from AllCells. CD4 + T cells were enriched from peripheral blood using the RosetteSep Human CD4 + T Cell Save | Full Paper |
2017 | Kawamura, S;Onai, N;Miya, F;Sato, T;Tsunoda, T;Kurabayashi, K;Yotsumoto, S;Kuroda, S;Takenaka, K;Akashi, K;Ohteki, T; | Identification of a Human Clonogenic Progenitor with Strict Monocyte Differentiation Potential: A Counterpart of Mouse cMoPs | Immunity | 28514689 | Human UCB samples were provided by the Japanese Red Cross Kanto-Koshinetsu Cord Blood Bank, and human BM samples were purchased from AllCells. All experiments were Save | Full Paper |
2017 | Peterson, VM;Zhang, KX;Kumar, N;Wong, J;Li, L;Wilson, DC;Moore, R;McClanahan, TK;Sadekova, S;Klappenbach, JA; | Multiplexed quantification of proteins and transcripts in single cells | Nature biotechnology | 28854175 | Cryopreserved PBMCs from a healthy donor (AllCells, PB003F, LotA5051) were thawed at 37 C for 3 min and resuspended in 10 ml of PBS with 1% Bovine Serum Albumin (BSA) ( Save | Full Paper |
2017 | Zheng, C;Zheng, L;Yoo, JK;Guo, H;Zhang, Y;Guo, X;Kang, B;Hu, R;Huang, JY;Zhang, Q;Liu, Z;Dong, M;Hu, X;Ouyang, W;Peng, J;Zhang, Z; | Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing | Cell | 28622514 | Anti-FOXP3 antibody (IHC) Abcam Cat#ab22510 CD3 Functional Grade Monoclonal Antibody eBioscience Cat#16-0037-85 CD28 Functional Grade Monoclonal Antibody eBioscience Cat#16-0289-85 Biological Samples Human PBMC AllCells https://www.allcells.com | Full Paper |
2017 | Sinha, G; | The organoid architect | Science (New York, N.Y.) | 28839056 | After almost a decade of tedious experiments, Clevers's postdoc Nick Barker struckgold in 2007: He discovered that cells carrying a receptor named LGR5 give rise to allcells in mouse Save | Full Paper |
2017 | Stoeckius, M;Hafemeister, C;Stephenson, W;Houck-Loomis, B;Chattopadhyay, PK;Swerdlow, H;Satija, R;Smibert, P; | Simultaneous epitope and transcriptome measurement in single cells | Nature methods | 28759029 | ononuclear cells (CBMCs) were isolated from cord blood (New York Blood Center) as described25. Cells were kept on ice during and after isolation. Peripheral blood mononuclear cells were obtained from Allcells (USA). Comparing flow cytometry and CITE | Full Paper |
2017 | Seet, CS;He, C;Bethune, MT;Li, S;Chick, B;Gschweng, EH;Zhu, Y;Kim, K;Kohn, DB;Baltimore, D;Crooks, GM;Montel-Hagen, A; | Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoids | Nature methods | 28369043 | placental units from deliveries at UCLA. Bone marrow (BM) was obtained from healthy adult donors (ages 18-51) through discarded material from allogeneic BM donor harvests at UCLA or purchased from AllCells Inc. (Alameda, CA). G-CSF mobilized peripher | Full Paper |
2017 | Mumbach, MR;Satpathy, AT;Boyle, EA;Dai, C;Gowen, BG;Cho, SW;Nguyen, ML;Rubin, AJ;Granja, JM;Kazane, KR;Wei, Y;Nguyen, T;Greenside, PG;Corces, MR;Tycko, J;Simeonov, DR;Suliman, N;Li, R;Xu, J;Flynn, RA;Kundaje, A;Khavari, PA;Marson, A;Corn, JE;Quertermous, T;Greenleaf, WJ;Chang, HY; | Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements | Nature genetics | 28945252 | Normal donor human peripheral blood cells were obtained fresh from AllCells. CD4 + T cells were enriched from peripheral blood using RosetteSep Human CD4 + T Cell Enrichment Save | Full Paper |
2017 | Hao, H;Hu, S;Chen, H;Bu, D;Zhu, L;Xu, C;Chu, F;Huo, X;Tang, Y;Sun, X;Ding, BS;Liu, DP;Hu, S;Wang, M; | Loss of Endothelial CXCR7 Impairs Vascular Homeostasis and Cardiac Remodeling After Myocardial Infarction: Implications for Cardiovascular Drug Discovery | Circulation | 28154007 | Human umbilical vein endothelial cells were obtained from AllCells. The CXCL12 concentration was determined with a human CXCL12 ELISA kit (R&D Systems). Calcium release was Save | Full Paper |
2017 | Gong, X;Litchfield, LM;Webster, Y;Chio, LC;Wong, SS;Stewart, TR;Dowless, M;Dempsey, J;Zeng, Y;Torres, R;Boehnke, K;Mur, C;Marugán, C;Baquero, C;Yu, C;Bray, SM;Wulur, IH;Bi, C;Chu, S;Qian, HR;Iversen, PW;Merzoug, FF;Ye, XS;Reinhard, C;De Dios, A;Du, J;Caldwell, CW;Lallena, MJ;Beckmann, RP;Buchanan, SG; | Genomic Aberrations that Activate D-type Cyclins Are Associated with Enhanced Sensitivity to the CDK4 and CDK6 Inhibitor Abemaciclib | Cancer cell | 29232554 | All cell lines were obtained from commercial vendors and were cultured in conditions recommended by vendors (Table S8; AllCells: https://www.allcells.com/; ATCC: https://www.atcc.org/; CASTCC: http://www.cellbank.org.cn; Chempartner: | Full Paper |
2017 | McKeown, MR;Corces, MR;Eaton, ML;Fiore, C;Lee, E;Lopez, JT;Chen, MW;Smith, D;Chan, SM;Koenig, JL;Austgen, K;Guenther, MG;Orlando, DA;Lovén, J;Fritz, CC;Majeti, R; | Superenhancer Analysis Defines Novel Epigenomic Subtypes of Non-APL AML, Including an RARα Dependency Targetable by SY-1425, a Potent and Selective RARα Agonist | Cancer discovery | 28729405 | Normal donor human blood cells were obtained fresh from AllCells (Alameda, CA) and FACS-enriched. Human AML samples were obtained from patients at the Stanford Medical Center with informed consent, according to Institutional Review Board (IRB)-approv | Full Paper |
2017 | Glass, JL;Hassane, D;Wouters, BJ;Kunimoto, H;Avellino, R;Garrett-Bakelman, FE;Guryanova, OA;Bowman, R;Redlich, S;Intlekofer, AM;Meydan, C;Qin, T;Fall, M;Alonso, A;Guzman, ML;Valk, PJM;Thompson, CB;Levine, R;Elemento, O;Delwel, R;Melnick, A;Figueroa, ME; | Epigenetic Identity in AML Depends on Disruption of Nonpromoter Regulatory Elements and Is Affected by Antagonistic Effects of Mutations in Epigenetic Modifiers | Cancer discovery | 28408400 | based on sample availability. Twenty-two normal CD34+ bone marrow specimens were obtained from AllCells (Alameda, CA). In addition, 6 AML samples were obtained from the Memorial Sloan Kettering Hematologic Oncology Tissue Bank for colony forming assa | Full Paper |
2017 | Pandya, H;Shen, MJ;Ichikawa, DM;Sedlock, AB;Choi, Y;Johnson, KR;Kim, G;Brown, MA;Elkahloun, AG;Maric, D;Sweeney, CL;Gossa, S;Malech, HL;McGavern, DB;Park, JK; | Differentiation of human and murine induced pluripotent stem cells to microglia-like cells | Nature neuroscience | 28253233 | RNA from human dendritic and bone marrow macrophages (AllCells) was also used. Isolated total RNA was amplified with the WT Expression Kit (Ambion) and labeled with the Save | Full Paper |
2017 | Rozo, C;Chinenov, Y;Maharaj, RK;Gupta, S;Leuenberger, L;Kirou, KA;Bykerk, VP;Goodman, SM;Salmon, JE;Pernis, AB; | Targeting the RhoA-ROCK pathway to reverse T-cell dysfunction in SLE | Annals of the rheumatic diseases | 28283529 | In separate experiments, cryopreserved human cord blood CD4 + T lymphocytes from different donors were obtained from Allcells, LLC. CD4 + T-cell purity was >95%. Cells were Save | Full Paper |
2017 | Miyawaki, K;Iwasaki, H;Jiromaru, T;Kusumoto, H;Yurino, A;Sugio, T;Uehara, Y;Odawara, J;Daitoku, S;Kunisaki, Y;Mori, Y;Arinobu, Y;Tsuzuki, H;Kikushige, Y;Iino, T;Kato, K;Takenaka, K;Miyamoto, T;Maeda, T;Akashi, K; | Identification of unipotent megakaryocyte progenitors in human hematopoiesis | Blood | 28336526 | Human adult bone marrow (BM) samples were obtained from healthy donors or purchased from AllCells. BM samples of adult hematological malignancies, diagnosed according to Save | Full Paper |
2017 | Leong, SR;Sukumaran, S;Hristopoulos, M;Totpal, K;Stainton, S;Lu, E;Wong, A;Tam, L;Newman, R;Vuillemenot, BR;Ellerman, D;Gu, C;Mathieu, M;Dennis, MS;Nguyen, A;Zheng, B;Zhang, C;Lee, G;Chu, YW;Prell, RA;Lin, K;Laing, ST;Polson, AG; | An anti-CD3/anti-CLL-1 bispecific antibody for the treatment of acute myeloid leukemia | Blood | 27908880 | (CD) CD8+ T cells were purified from peripheral blood of a healthy human donor (ALLCells, Alameda, CA) for donor 1 and a Genentech donor for donor 2 by Ficoll density gradient Save | Full Paper |
2017 | Weiss, M; | Genome Editing Strategies to Treat Beta-Hemoglobinopathies | Blood | Frozen CD34+ cells from mobilized peripheral blood of normal individuals were purchased from AllCells. Peripheral blood CD34+ cells were acquired from SCD patients undergoing therapeutic red cell exchange at the Cancer & Hematology Centers, Texas Chi | Full Paper | |
2017 | Tisdale, J;Pierciey, F;Kamble, R;Kanter, J;Krishnamurti, L;Kwiatkowski, J;Thompson, A;Shestopalov, I;Bonner, M;Joseney-Antoine, M;Asmal, M;Walters, M; | Successful Plerixafor-Mediated Mobilization, Apheresis, and Lentiviral Vector Transduction of Hematopoietic Stem Cells in Patients with Severe Sickle Cell Disease | Blood | Background Patients with severe sickle cell disease (SCD) may benefit from β-globin gene transfer into autologous hematopoietic stem cells (HSC). Successful HBB gene transfer requires vector-mediated transduction of primitive HSCs. Ste | Full Paper | |
2017 | Fujii, S;Miura, Y;Fujishiro, A;Shindo, T;Shimazu, Y; | Human Bone Marrow Mesenchymal Stromal/Stem Cell-Derived Extracellular Vesicles Ameliorate Graft-Versus-Host Disease-Related Manifestations | Blood | Human MSC were isolated from BM samples purchased from AllCells (Alameda, CA) based on our previously published method (Biochem Biophys Res Commun 2016;469:823). EV Save | Full Paper | |
2017 | Mathiyalagan, P;Liang, Y;Kim, D;Misener, S;Thorne, T;Kamide, CE;Klyachko, E;Losordo, DW;Hajjar, RJ;Sahoo, S; | Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic Hindlimb | Circulation research | 28298297 | The cells were purified from mobilized peripheral blood mononuclear cells (AllCells LLC, Emeryville, CA) with an Isolex 300i device (Baxter Healthcare); cell purity (85% to 95%) was Save | Full Paper |
2017 | Elagib, KE;Lu, CH;Mosoyan, G;Khalil, S;Zasadzińska, E;Foltz, DR;Balogh, P;Gru, AA;Fuchs, DA;Rimsza, LM;Verhoeyen, E;Sansó, M;Fisher, RP;Iancu-Rubin, C;Goldfarb, AN; | Neonatal expression of RNA-binding protein IGF2BP3 regulates the human fetal-adult megakaryocyte transition | The Journal of clinical investigation | 28481226 | The normal donor samples purchased from the Fred Hutchinson Cancer Research Center and AllCells were originally obtained with informed consent and IRB approval at both Save | Full Paper |
2017 | Chung, SS;Eng, WS;Hu, W;Khalaj, M;Garrett-Bakelman, FE;Tavakkoli, M;Levine, RL;Carroll, M;Klimek, VM;Melnick, AM;Park, CY; | CD99 is a therapeutic target on disease stem cells in myeloid malignancies | Science translational medicine | 28123069 | consent under IRB-approved protocols. Umbilical cord blood (CB) specimens were purchased from the New York Blood Center (New York, New York). Normal human BM specimens were purchased from AllCells Inc. (Emeryville, California) | Full Paper |
2017 | Zhang, T;Chi, S;Jiang, F;Zhao, Q;Xiao, B; | A protein interaction mechanism for suppressing the mechanosensitive Piezo channels | Nature communications | 29176668 | (MEM) containing 10% FBS, non-essential amino acids, 1 mM sodium pyruvate, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin. Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured using EGM-2 gro | Full Paper |
2017 | Trabanelli, S;Chevalier, MF;Martinez-Usatorre, A;Gomez-Cadena, A;Salomé, B;Lecciso, M;Salvestrini, V;Verdeil, G;Racle, J;Papayannidis, C;Morita, H;Pizzitola, I;Grandclément, C;Bohner, P;Bruni, E;Girotra, M;Pallavi, R;Falvo, P;Leibundgut, EO;Baerlocher, GM;Carlo-Stella, C;Taurino, D;Santoro, A;Spinelli, O;Rambaldi, A;Giarin, E;Basso, G;Tresoldi, C;Ciceri, F;Gfeller, D;Akdis, CA;Mazzarella, L;Minucci, S;Pelicci, PG;Marcenaro, E;McKenzie, ANJ;Vanhecke, D;Coukos, G;Mavilio, D;Curti, A;Derré, L;Jandus, C; | Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axis | Nature communications | 28928446 | Human CD34 + cells were purchased from either Lonza (CH) or AllCells (USA). NSG mice were purchased from The Jackson Laboratory (stock number: 005557) and bred, treated and Save | Full Paper |
2017 | Zheng, GX;Terry, JM;Belgrader, P;Ryvkin, P;Bent, ZW;Wilson, R;Ziraldo, SB;Wheeler, TD;McDermott, GP;Zhu, J;Gregory, MT;Shuga, J;Montesclaros, L;Underwood, JG;Masquelier, DA;Nishimura, SY;Schnall-Levin, M;Wyatt, PW;Hindson, CM;Bharadwaj, R;Wong, A;Ness, KD;Beppu, LW;Deeg, HJ;McFarland, C;Loeb, KR;Valente, WJ;Ericson, NG;Stevens, EA;Radich, JP;Mikkelsen, TS;Hindson, BJ;Bielas, JH; | Massively parallel digital transcriptional profiling of single cells | Nature communications | 28091601 | s Jurkat (ATCC TIB-152), 293T (ATCC CRL-11268) and 3T3 (ATCC CRL-1658) cells were acquired from ATCC and cultured according to ATCC guidelines. Fresh PBMCs, frozen PBMCs and BMMCs were purchased from ALLCELLS. Frozen PBMCs from Donor A were made from | Full Paper |
2017 | Nakata, R;Shimada, H;Fernandez, GE;Fanter, R;Fabbri, M;Malvar, J;Zimmermann, P;DeClerck, YA; | Contribution of neuroblastoma-derived exosomes to the production of pro-tumorigenic signals by bone marrow mesenchymal stromal cells | Journal of extracellular vesicles | 28717423 | Human BM-derived MSCs were purchased from AllCells or isolated in our laboratory from the BM of patients with NB using a previously published protocol [10]. Cells were maintained Save | Full Paper |
2017 | Burton, OT;Stranks, AJ;Tamayo, JM;Koleoglou, KJ;Schwartz, LB;Oettgen, HC; | A humanized mouse model of anaphylactic peanut allergy | The Journal of allergy and clinical immunology | 27417025 | Cord blood-derived flow-sorted human CD34+ hematopoietic stem cells (HSCs) were purchased from AllCells (Alameda, Calif). Such cells are obtained under Institutional Review Board- or Human Subject Committee-approved protocols. Cells (5 × 104-105) wer | Full Paper |
2017 | Bolisetty, M;Stitzel, M;Robson, P; | CellView: Interactive exploration of high dimensional single cell RNA-seq data | bioRxiv | Single cell RNA-seq - PBMCs were purchased from AllCells, thawed quickly at 37C and into DMEM supplemented with 10% FBS. Cells were quickly spun down at 400g, for 10min. Save | Full Paper | |
2017 | Li, S;Siriwon, N;Zhang, X;Yang, S;Jin, T;He, F;Kim, YJ;Mac, J;Lu, Z;Wang, S;Han, X;Wang, P; | Enhanced Cancer Immunotherapy by Chimeric Antigen Receptor-Modified T Cells Engineered to Secrete Checkpoint Inhibitors | Clinical cancer research : an official journal of the American Association for Cancer Research | 28912137 | Chimeric antigen receptor (CAR) T-cell based therapy has shown promise as an immunotherapeutic modality for cancers. It has achieved successful responses in patients with certain Save | Full Paper |
2017 | Dai, X;Yan, J;Fu, X;Pan, Q;Sun, D;Xu, Y;Wang, J;Nie, L;Tong, L;Shen, A;Zheng, M;Huang, M;Tan, M;Liu, H;Huang, X;Ding, J;Geng, M; | Aspirin Inhibits Cancer Metastasis and Angiogenesis via Targeting Heparanase | Clinical cancer research : an official journal of the American Association for Cancer Research | 28710312 | from the Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences); primary human umbilical vascular endothelial cells (HUVECs) were purchased from AllCells. CHO- Save | Full Paper |
2017 | Das, DS;Das, A;Ray, A;Song, Y;Samur, MK;Munshi, NC;Chauhan, D;Anderson, KC; | Blockade of Deubiquitylating Enzyme USP1 Inhibits DNA Repair and Triggers Apoptosis in Multiple Myeloma Cells | Clinical cancer research : an official journal of the American Association for Cancer Research | 28270494 | Purpose: The ubiquitin proteasome pathway is a validated therapeutic target in multiple myeloma. Deubiquitylating enzyme USP1 participates in DNA damage response and cellular Save | Full Paper |
2017 | Liu, L;Lam, CK;Long, V;Widjaja, L;Yang, Y;Li, H;Jin, L;Burke, S;Gorlatov, S;Brown, J;Alderson, R;Lewis, MD;Nordstrom, JL;Koenig, S;Moore, PA;Johnson, S;Bonvini, E; | MGD011, A CD19 x CD3 Dual-Affinity Retargeting Bi-specific Molecule Incorporating Extended Circulating Half-life for the Treatment of B-Cell Malignancies | Clinical cancer research : an official journal of the American Association for Cancer Research | 27663593 | Cryopreserved purified primary CLL patient samples were from AllCells, LLC. Heparinized whole blood from cynomolgus monkeys was from Worldwide Primates, Inc. Save | Full Paper |
2017 | DeRyckere, D;Lee-Sherick, AB;Huey, MG;Hill, AA;Tyner, JW;Jacobsen, KM;Page, LS;Kirkpatrick, GG;Eryildiz, F;Montgomery, SA;Zhang, W;Wang, X;Frye, SV;Earp, HS;Graham, DK; | UNC2025, a MERTK Small-Molecule Inhibitor, Is Therapeutically Effective Alone and in Combination with Methotrexate in Leukemia Models | Clinical cancer research : an official journal of the American Association for Cancer Research | 27649555 | Deidentified cord blood and normal bone marrow samples were obtained commercially from Clinimmune Labs and ALLCELLS, respectively. Save | Full Paper |
2017 | Stoeckius, M;Hafemeister, C;Stephenson, W;Houck-Loomis, B;Chattopadhyay, P;Swerdlow, H;Satija, R;Smibert, P; | Large-scale simultaneous measurement of epitopes and transcriptomes in single cells | bioRxiv | Cord blood mononuclear cells (CBMCs) were isolated from cord blood (New York Blood Center) as described30. Cells were kept on ice during and after isolation. Peripheral blood mononuclear cells were obtained from Allcells (USA) | Full Paper | |
2017 | Cichocki, F;Valamehr, B;Bjordahl, R;Zhang, B;Rezner, B;Rogers, P;Gaidarova, S;Moreno, S;Tuininga, K;Dougherty, P;McCullar, V;Howard, P;Sarhan, D;Taras, E;Schlums, H;Abbot, S;Shoemaker, D;Bryceson, YT;Blazar, BR;Wolchko, S;Cooley, S;Miller, JS; | GSK3 Inhibition Drives Maturation of NK Cells and Enhances Their Antitumor Activity | Cancer research | 28790065 | Peripheral blood mononuclear cells (PBMCs) from healthy CMV seropositive donors were obtained from Memorial Blood Bank (Minneapolis, MN), AllCells Inc. (Alameda, CA) and Key Biologics (Memphis, TN). All samples obtained with written consent and were | Full Paper |
2017 | Jiang, H;Rivera-Molina, Y;Gomez-Manzano, C;Clise-Dwyer, K;Bover, L;Vence, LM;Yuan, Y;Lang, FF;Toniatti, C;Hossain, MB;Fueyo, J; | Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer Vaccination | Cancer research | 28566332 | Oncolytic viruses selectively lyse tumor cells, disrupt immunosuppression within the tumor, and reactivate antitumor immunity, but they have yet to live up to their therapeutic potential. Save | Full Paper |
2017 | Chen, Y;Shan, Y;Lu, M;DeSouza, N;Guo, Z;Hoffman, R;Liang, A;Li, S; | Alox5 Blockade Eradicates JAK2V617F-Induced Polycythemia Vera in Mice | Cancer research | 27784744 | GE Healthcare) and low-density mononuclear cells were separated after centrifugation. The CD34+ cell population was isolated using a human CD34+ cell selection kit (StemCell Technologies) according to the manufacturer's instructions. Normal human CD3 | Full Paper |
2017 | Egidio, C;Gonzales, M;Brockman, J;Chen, S;Durruthy-Durruthy, R;Rogers, C;Ray, M; | Abstract LB-078: Improved single-cell mRNA sequencing for transcriptome and paired-chain TCR analysis of primary human CD3+ T cells | Cancer Research | The low RNA content of single human T cells has limited the success of single-cell transcriptome profiling, an approach that can generate data to reconstruct paired α,β T cell receptor (TCR) chains¹. Single-cell TCR sequencing is a powerful molecular | Full Paper | |
2017 | Chen, Q;Lawrenz, A;Wang, A;Kelly, E;Harberts, E;Ernst, R;Tong, J; | Abstract 4021: Donor-specific primary monocyte-derived dendritic cell maturation model for high-throughput screening of immunostimulatory adjuvants | Cancer Research | 1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++2University of Maryland-Baltimore, Baltimore, MD.++2University of Maryland-Baltimore, Baltimore, MD.++1AllCells, Alameda, CA | Full Paper | |
2017 | Han, X;Cinay, GE;Zhao, Y;Guo, Y;Zhang, X;Wang, P; | Adnectin-Based Design of Chimeric Antigen Receptor for T Cell Engineering | Molecular therapy : the journal of the American Society of Gene Therapy | 28784559 | Human PBMCs from healthy donors were obtained from AllCells. PBMCs were cultured in T cell medium (TCM) consisting of X-Vivo 15 (Lonza) supplemented with 5% human AB serum Save | Full Paper |
2017 | Tran, R;Myers, DR;Denning, G;Shields, JE;Lytle, AM;Alrowais, H;Qiu, Y;Sakurai, Y;Li, WC;Brand, O;Le Doux, JM;Spencer, HT;Doering, CB;Lam, WA; | Microfluidic Transduction Harnesses Mass Transport Principles to Enhance Gene Transfer Efficiency | Molecular therapy : the journal of the American Society of Gene Therapy | 28780274 | vices and wells for at least 72 hr before assessing GFP expression of transduced cells with a BD C6 Accuri flow cytometer. Primary Human T Cell Culture Frozen human pan T cells were purchased from AllCells. Cells were thawed following the AllCells t | Full Paper |
2017 | Han, X;Bryson, PD;Zhao, Y;Cinay, GE;Li, S;Guo, Y;Siriwon, N;Wang, P; | Masked Chimeric Antigen Receptor for Tumor-Specific Activation | Molecular therapy : the journal of the American Society of Gene Therapy | 28129121 | Frozen human peripheral blood mononuclear cells (PBMCs) were obtained from AllCells. PBMCs were thawed in TCM and rested overnight. Before retroviral transduction, PBMCs were Save | Full Paper |
2017 | Liu, X;Rothe, K;Yen, R;Fruhstorfer, C;Maetzig, T;Chen, M;Forrest, DL;Humphries, RK;Jiang, X; | A novel AHI-1-BCR-ABL-DNM2 complex regulates leukemic properties of primitive CML cells through enhanced cellular endocytosis and ROS-mediated autophagy | Leukemia | 28366933 | PB or BM cells were obtained from newly diagnosed patients and healthy adult donors (ALLCELLS). Informed consent was obtained in accordance with the Declaration of Helsinki, and Save | Full Paper |
2017 | Mosoyan, G;Kraus, T;Ye, F;Eng, K;Crispino, JD;Hoffman, R;Iancu-Rubin, C; | Imetelstat, a telomerase inhibitor, differentially affects normal and malignant megakaryopoiesis | Leukemia | 28270692 | Human MKs were generated using a two-step liquid culture system of either bone marrow (BM) or peripheral blood (PB) derived CD34+ cells (AllCells, LLC, Emeryville, CA, USA) as previously described.21,22 CFU-MK assays were performed by using the MegaC | Full Paper |
2017 | de Vasconcellos, JF;Tumburu, L;Byrnes, C;Lee, YT;Xu, PC;Li, M;Rabel, A;Clarke, BA;Guydosh, NR;Proia, RL;Miller, JL; | IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblasts | Proceedings of the National Academy of Sciences of the United States of America | 28652347 | Digestive and Kidney Diseases Institutional Intramural Review Board. CD34(+) cells from adult human healthy volunteers and human cord blood CD34(+) cells obtained from AllCells were studied ex vivo using a serum-free 21-day culture system as previous | Full Paper |
2017 | Klinker, MW;Marklein, RA;Lo Surdo, JL;Wei, CH;Bauer, SR; | Morphological features of IFN-γ-stimulated mesenchymal stromal cells predict overall immunosuppressive capacity | Proceedings of the National Academy of Sciences of the United States of America | 28283659 | PBMCs used in this study (AllCells; PB006F) were collected from healthy adult human donors. To activate T cells, 10 5 beads coated with anti-CD3 and anti-CD28 (Life Technologies; Save | Full Paper |
2017 | Frescas, D;Roux, CM;Aygun-Sunar, S;Gleiberman, AS;Krasnov, P;Kurnasov, OV;Strom, E;Virtuoso, LP;Wrobel, M;Osterman, AL;Antoch, MP;Mett, V;Chernova, OB;Gudkov, AV; | Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibody | Proceedings of the National Academy of Sciences of the United States of America | 28193858 | Human NDFs were purchased from AllCells and maintained in DMEM supplemented with 10% (vol/vol) FBS serum, 100 units/mL of penicillin, 100 µg/mL of streptomycin, and 2 mM l- Save | Full Paper |
2017 | Zhang, H;Gregorio, JD;Iwahori, T;Zhang, X;Choi, O;Tolentino, LL;Prestwood, T;Carmi, Y;Engleman, EG; | A distinct subset of plasmacytoid dendritic cells induces activation and differentiation of B and T lymphocytes | Proceedings of the National Academy of Sciences of the United States of America | 28167780 | Stanford Blood Center. Human cord blood and bone marrow samples were purchased from AllCells. Human tonsil cells were obtained from the Stanford Tissue Bank. Human subjects protocols were approved by Stanford’s Institutional Review Board (Palo Alto, | Full Paper |
2017 | Nada, MH;Wang, H;Workalemahu, G;Tanaka, Y;Morita, CT; | Enhancing adoptive cancer immunotherapy with Vγ2Vδ2 T cells through pulse zoledronate stimulation | Journal for immunotherapy of cancer | 28239463 | random healthy adult donors were obtained by density centrifugation over Ficoll-Hypaque (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) of either random donor leukopaks (12 donors) (obtained from AllCells, LLC., Alameda, CA or the Blood Donor Cent | Full Paper |
2017 | Kuramitsu, M;Sekizuka, T;Yamochi, T;Firouzi, S;Sato, T;Umeki, K;Sasaki, D;Hasegawa, H;Kubota, R;Sobata, R;Matsumoto, C;Kaneko, N;Momose, H;Araki, K;Saito, M;Nosaka, K;Utsunomiya, A;Koh, KR;Ogata, M;Uchimaru, K;Iwanaga, M;Sagara, Y;Yamano, Y;Okayama, A;Miura, K;Satake, M;Saito, S;Itabashi, K;Yamaguchi, K;Kuroda, M;Watanabe, T;Okuma, K;Hamaguchi, I; | Proviral Features of Human T Cell Leukemia Virus Type 1 in Carriers with Indeterminate Western Blot Analysis Results | Journal of clinical microbiology | 28701419 | AllCells Inc. (Alameda, CA, USA). Cryopreserved PBMCs were resuspended in RPMI 1640 supplemented with 10% FBS at 37C in accordance with the protocol provided by AllCells Inc. Save | Full Paper |
2017 | Gonçalves Silva, I;Yasinska, IM;Sakhnevych, SS;Fiedler, W;Wellbrock, J;Bardelli, M;Varani, L;Hussain, R;Siligardi, G;Ceccone, G;Berger, SM;Ushkaryov, YA;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV; | The Tim-3-galectin-9 Secretory Pathway is Involved in the Immune Escape of Human Acute Myeloid Leukemia Cells | EBioMedicine | 28750861 | Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordance with the Save;ultured in Stem-Pro-34 serum-free media in the presence of 100 ng/ml SCF (Kirshenba | Full Paper |
2017 | Alvey, CM;Spinler, KR;Irianto, J;Pfeifer, CR;Hayes, B;Xia, Y;Cho, S;Dingal, PCPD;Hsu, J;Smith, L;Tewari, M;Discher, DE; | SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid Tumors | Current biology : CB | 28669759 | Subject 1: Female age 30, subject 2: Female age 31, subject 3: Male age 31, subject 4: Male age 22 with additional details provided by AllCells per (Figure 7A). Marrow was pooled and Save | Full Paper |
2017 | Fujishiro, A;Miura, Y;Iwasa, M;Fujii, S;Sugino, N;Andoh, A;Hirai, H;Maekawa, T;Ichinohe, T; | Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cells | Inflammation and regeneration | 29259718 | e 5E10), and anti-CD105 (clone SN6) antibodies were from eBioscience (San Diego, CA). Isolation, expansion, and γ-irradiation of BM-MSCs BM samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA). BM-MSCs were isolated ba | Full Paper |
2017 | Yang, C;Wang, X;Ma, B;Zhu, H;Huan, Z;Ma, N;Wu, C;Chang, J; | 3D-Printed Bioactive Ca3SiO5 Bone Cement Scaffolds with Nano Surface Structure for Bone Regeneration | ACS applied materials & interfaces | 28117976 | Rat bone-marrow stem cells (rBMSCs) (obtained from Allcells, Silicon Valley, CA) were seeded with an initial density of 1× 104 on each scaffold in 48 well tissue culture plates containing 500 μL of Dulbecco’s modified Eagle’s medium (DMEM, Gibco) sup | Full Paper |
2017 | Gautron, AS;Juillerat, A;Guyot, V;Filhol, JM;Dessez, E;Duclert, A;Duchateau, P;Poirot, L; | Fine and Predictable Tuning of TALEN Gene Editing Targeting for Improved T Cell Adoptive Immunotherapy | Molecular therapy. Nucleic acids | 29246309 | eBioscience. All the cytometry analyses were performed on a FACSCanto II (BD Biosciences). Cell Culture Frozen peripheral blood mononuclear cells (PBMC) were obtained from healthy volunteer donors (Allcells). T lymphocytes were activated directly fr | Full Paper |
2017 | Shim, H; | Hypoxia-Inducible Factor-1 | Encyclopedia of Cancer | In brief, CD34-depleted human cord blood mononuclear cells (#CB117; AllCells) were infected in vitro with the M81 strain of EBV (2,000 GRU) by incubation at 37C for 1.5 h after which Save | Full Paper | |
2017 | Zhang, W;Zhou, L;Shi, J;Deng, H; | Fabrication of novel visible-light-driven AgI/g-C(3)N(4) composites with enhanced visible-light photocatalytic activity for diclofenac degradation | Journal of colloid and interface science | 28222304 | Diclofenac sodium (DCF) was purchased from the Sigma Chemical Reagent Co., US Human umbilical vein endothelial cells (HUVECs) were obtained from Allcells, Shanghai, China. Save | Full Paper |
2017 | Shi, J;Deng, H;Pan, H;Xu, Y;Zhang, M; | Epigallocatechin-3-gallate attenuates microcystin-LR induced oxidative stress and inflammation in human umbilical vein endothelial cells | Chemosphere | 27776235 | Primary HUVECs were obtained from Allcells (Shanghai, China) and cells in passages 3-5 were used in this study. Cells were grown in an RPMI 1640 medium containing 20% FBS ( Save | Full Paper |
2017 | Ali, AJ;Abuelela, AF;Merzaban, JS; | An Analysis of Trafficking Receptors Shows that CD44 and P-Selectin Glycoprotein Ligand-1 Collectively Control the Migration of Activated Human T-Cells | Frontiers in immunology | 28515724 | Frozen normal peripheral blood mononuclear cells isolated from subjects with and without psoriasis were ordered from AllCells (CA, USA). Naive Pan T-cell Isolation Kit and Pan T-cell Save | Full Paper |
2017 | Guo, H;Zhu, Q;Yu, X;Merugu, SB;Mangukiya, HB;Smith, N;Li, Z;Zhang, B;Negi, H;Rong, R;Cheng, K;Wu, Z;Li, D; | Tumor-secreted anterior gradient-2 binds to VEGF and FGF2 and enhances their activities by promoting their homodimerization | Oncogene | 28481872 | HUVECs were purchased from AllCells Company and maintained in HUVEC complete medium (AllCells, Shanghai, China). Cells were passaged for fewer than six passages. THP-1 Save | Full Paper |
2017 | Winter, MB;La Greca, F;Arastu-Kapur, S;Caiazza, F;Cimermancic, P;Buchholz, TJ;Anderl, JL;Ravalin, M;Bohn, MF;Sali, A;O'Donoghue, AJ;Craik, CS; | Immunoproteasome functions explained by divergence in cleavage specificity and regulation | eLife | 29182146 | on the list of commonly misidentified cell lines from the International Cell Line Authentication Committee. Primary human peripheral blood mononuclear cells (PBMCs) (cat. PB005F) were purchased from AllCells (Alameda, CA). The SUP-B15 cell line was p | Full Paper |
2017 | Bak, RO;Dever, DP;Reinisch, A;Cruz Hernandez, D;Majeti, R;Porteus, MH; | Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR/Cas9 and AAV6 | eLife | 28956530 | Frozen CD34+ HSPCs derived from mobilized peripheral blood or cord blood were purchased from AllCells (Alameda, CA, USA) and thawed according to manufacturer's instructions. Save | Full Paper |
2017 | Kieffer, C;Ladinsky, MS;Ninh, A;Galimidi, RP;Bjorkman, PJ; | Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomography | eLife | 28198699 | s used at a dilution of 1:500 and 10 nm gold-conjugated goat anti-rabbit IgG (Ted Pella) was used at a dilution of 1:20. Flow cytometry of primary and reconstituted human PBMCs Cultured human PBMCs (AllCells, inc.) were analyzed by flow cytometry (M | Full Paper |
2017 | Li, Y;Li, X;Zhao, R;Wang, C;Qiu, F;Sun, B;Ji, H;Qiu, J;Wang, C; | Enhanced adhesion and proliferation of human umbilical vein endothelial cells on conductive PANI-PCL fiber scaffold by electrical stimulation | Materials science & engineering. C, Materials for biological applications | 28024565 | Human umbilical vein endothelial cells (HUVECs, AllCells Biological Technology Co., Ltd. Shanghai) were cultured in complete Medium. During the cell culture, the culture medium was refreshed every 2- 3 days. HUVECs at passage 2- 3 were used in this w | Full Paper |
2017 | Xu, JG;Zhu, SY;Heng, BC;Dissanayaka, WL;Zhang, CF; | TGF-β1-induced differentiation of SHED into functional smooth muscle cells | Stem cell research & therapy | 28114966 | f this study was to differentiate SHED into SMCs by TGF-β1 alone or in combination with BMP4, and to investigate the functionality of the derived SMCs. Methods Cell culture SHED were purchased from ALLCELLS (Alameda, CA, USA), and were evaluated for | Full Paper |
2017 | Schlatzer, D;Haqqani, AA;Li, X;Dobrowolski, C;Chance, MR;Tilton, JC; | A Targeted Mass Spectrometry Assay for Detection of HIV Gag Protein Following Induction of Latent Viral Reservoirs | Analytical chemistry | 28467046 | Normal donor samples were obtained from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated using RosetteSep CD4+ T cell enrichment kit Save | Full Paper |
2017 | Tan, S;Shan, Y;Wang, Y;Lin, Y;Liao, S;Deng, Z;Zhou, L;Cai, W;Zeng, Q;Zhang, L;Zhang, B;Men, X;Li, H;Hu, X;Wu, C;Peng, L;Lu, Z; | Exacerbation of oxygen-glucose deprivation-induced blood-brain barrier disruption: potential pathogenic role of interleukin-9 in ischemic stroke | Clinical science (London, England : 1979) | 28550144 | The bEnd.3 cells were purchased from AllCells and grown to subconfluence before the exposure to oxygen-glucose deprivation (OGD: 94.95% N2, 0.05% O2, 5% CO2) or normoxic conditions (75% N2, 20% O2, 5% CO2) for 4 or 24 h. Cells were divided into the f | Full Paper |
2017 | Gregg, KA;Harberts, E;Gardner, FM;Pelletier, MR;Cayatte, C;Yu, L;McCarthy, MP;Marshall, JD;Ernst, RK; | Rationally Designed TLR4 Ligands for Vaccine Adjuvant Discovery | mBio | 28487429 | The dendritic cell (DC) maturation experiments were contracted to and run by AllCells (Alameda, CA) using standard company protocols. Briefly, human PBMCs from 3 separate donors Save | Full Paper |
2017 | Wang, K;Jiang, Z;Webster, KA;Chen, J;Hu, H;Zhou, Y;Zhao, J;Wang, L;Wang, Y;Zhong, Z;Ni, C;Li, Q;Xiang, C;Zhang, L;Wu, R;Zhu, W;Yu, H;Hu, X;Wang, J; | Enhanced Cardioprotection by Human Endometrium Mesenchymal Stem Cells Driven by Exosomal MicroRNA-21 | Stem cells translational medicine | 28170197 | ngzhou, China, http://www.sebio.net.cn). Human AdMSCs were purchased from Cyagen Bioscience (Guangzhou, China, http://www.cyagen.com). Human umbilical vein endothelial cells (HUVECs) were provided by AllCells (Shanghai, China, http://www.allcells.com | Full Paper |
2017 | Sebastian, NT;Zaikos, TD;Terry, V;Taschuk, F;McNamara, LA;Onafuwa-Nuga, A;Yucha, R;Signer, RAJ;Riddell, J;Bixby, D;Markowitz, N;Morrison, SJ;Collins, KL; | CD4 is expressed on a heterogeneous subset of hematopoietic progenitors, which persistently harbor CXCR4 and CCR5-tropic HIV proviral genomes in vivo | PLoS pathogens | 28732051 | from each donor. All collected samples were coded. Whole umbilical cord blood (CB) from uninfected donors was obtained from the New York Blood Center and whole bone marrow was obtained commercially (AllCells Ltd.). All collected samples were anonymi | Full Paper |
2017 | Kraus, RJ;Yu, X;Cordes, BA;Sathiamoorthi, S;Iempridee, T;Nawandar, DM;Ma, S;Romero-Masters, JC;McChesney, KG;Lin, Z;Makielski, KR;Lee, DL;Lambert, PF;Johannsen, EC;Kenney, SC;Mertz, JE; | Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoter | PLoS pathogens | 28617871 | In brief, CD34-depleted human cord blood mononuclear cells (#CB117; AllCells) were infected in vitro with the M81 strain of EBV (2,000 GRU) by incubation at 37C for 1.5 h after which Save | Full Paper |
2017 | Ganaie, SS;Zou, W;Xu, P;Deng, X;Kleiboeker, S;Qiu, J; | Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complex | PLoS pathogens | 28459842 | ti-viral prophylaxis of transplant recipients. Materials and methods Ethics statement We purchased CD34+ hematopoietic stem cells, which were isolated from bone marrow of a healthy human donor, from AllCells LLC (Alameda, CA) without any identificat | Full Paper |
2017 | Nie, Y;Ran, Y;Zhang, HY;Huang, ZF;Pan, ZY;Wang, SY;Wang, YY; | GPATCH3 negatively regulates RLR-mediated innate antiviral responses by disrupting the assembly of VISA signalosome | PLoS pathogens | 28414768 | University, Cambridge, MA). HEK293T (Transformed Human Embryonic Kidney 293 cell line, ATCC) cells, A549 (Human Lung Adenocarcinoma cell line, ATCC) cells, PBMCs (Peripheral Blood Mononuclear Cells, Allcells), Human recombinant TNFα (R&D Systems), po | Full Paper |
2017 | Xu, P;Zhou, Z;Xiong, M;Zou, W;Deng, X;Ganaie, SS;Kleiboeker, S;Peng, J;Liu, K;Wang, S;Ye, SQ;Qiu, J; | Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway | PLoS pathogens | 28264028 | We purchased the cells at AllCells LLC. (www.allcells.com, Alameda, CA), which were all anonymized, and, therefore, an IRB review was waived. Save | Full Paper |
2017 | Morin, J;Dubey, N;Decroix, F;Luong-Van, E;Castro Neto, A;Rosa, V; | Graphene transfer to 3-dimensional surfaces: a vacuum-assisted dry transfer method | 2D Materials | Dental pulp stem cells (DPSC) (AllCells, USA) of passage 4 were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen, USA), supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin/streptomycin (Invitrogen). Disinfection of the i | Full Paper | |
2017 | Ma, SD;Tsai, MH;Romero-Masters, JC;Ranheim, EA;Huebner, SM;Bristol, JA;Delecluse, HJ;Kenney, SC; | Latent Membrane Protein 1 (LMP1) and LMP2A Collaborate To Promote Epstein-Barr Virus-Induced B Cell Lymphomas in a Cord Blood-Humanized Mouse Model but Are Not Essential | Journal of virology | 28077657 | Commercially purchased CD34depleted human cord blood mononuclear cells (CB117; AllCells, LLC) were mock infected or infected with WT or mutant viruses in vitro for 1.5 h, and 12 Save | Full Paper |
2017 | Adler, AS;Mizrahi, RA;Spindler, MJ;Adams, MS;Asensio, MA;Edgar, RC;Leong, J;Leong, R;Roalfe, L;White, R;Goldblatt, D;Johnson, DS; | Rare, high-affinity anti-pathogen antibodies from human repertoires, discovered using microfluidics and molecular genomics | mAbs | 28846502 | For influenza A vaccinated libraries, we contracted AllCells to vaccinate three donors (ages For the pneumococcus vaccinated libraries, we contracted AllCells to vaccinate five donors ( Save | Full Paper |
2017 | Urbinati, F;Wherley, J;Geiger, S;Fernandez, BC;Kaufman, ML;Cooper, A;Romero, Z;Marchioni, F;Reeves, L;Read, E;Nowicki, B;Grassman, E;Viswanathan, S;Wang, X;Hollis, RP;Kohn, DB; | Preclinical studies for a phase 1 clinical trial of autologous hematopoietic stem cell gene therapy for sickle cell disease | Cytotherapy | 28733131 | Control healthy donor BM samples were purchased from commercial sources (AllCells or Hemacare) obtaining aspirates from volunteer donors. The ages of the five healthy donors Save | Full Paper |
2017 | Pachler, K;Lener, T;Streif, D;Dunai, ZA;Desgeorges, A;Feichtner, M;Öller, M;Schallmoser, K;Rohde, E;Gimona, M; | A Good Manufacturing Practice-grade standard protocol for exclusively human mesenchymal stromal cell-derived extracellular vesicles | Cytotherapy | 28188071 | The second BM aspirate was purchased from AllCells. Both donors had signed an informed consent. BM-MSCs were isolated from BM as described [30] and tested negative for Save | Full Paper |
2017 | Khairani, M;Nur, N;Basma, M;Widya, L;Solachuddin Jauhari, I;Deny, D;Azlina, A;Thirumulu, K; | Effects of flaxseed extract on the viability of stem cells from human extracted deciduous teeth (SHED) | Frontiers in Bioengineering and Biotechnology | SHED (AllCells, USA) was previously cultured and maintained in alpha-MEM (GIBCO, USA) containing 10% FBS (GIBCO, USA) and 0.5% penicillin/streptomycin (GIBCO, USA) until Save | Full Paper | |
2017 | Lifshitz, V;Priceman, SJ;Li, W;Cherryholmes, G;Lee, H;Makovski-Silverstein, A;Borriello, L;DeClerck, YA;Yu, H; | Sphingosine-1-Phosphate Receptor-1 Promotes Environment-Mediated and Acquired Chemoresistance | Molecular cancer therapeutics | 28716816 | Human BM-MSC were either purchased from ALLCells LLC or obtained from fresh ALLCells, which were maintained in basal medium with growth supplements obtained from ALLCells. Save | Full Paper |
2017 | Zhao, H;Gulesserian, S;Ganesan, SK;Ou, J;Morrison, K;Zeng, Z;Robles, V;Snyder, J;Do, L;Aviña, H;Karki, S;Stover, DR;Doñate, F; | Inhibition of Megakaryocyte Differentiation by Antibody-Drug Conjugates (ADCs) is Mediated by Macropinocytosis: Implications for ADC-induced Thrombocytopenia | Molecular cancer therapeutics | 28655784 | ABM026F) isolated from bone marrow were purchased from ALLCELLS. All HSC growth media (StemSpan SFEM II, catalog no. 09600), supplements for expansion (CC100, catalog no. Save | Full Paper |
2017 | Ao, JY;Zhu, XD;Chai, ZT;Cai, H;Zhang, YY;Zhang, KZ;Kong, LQ;Zhang, N;Ye, BG;Ma, DN;Sun, HC; | Colony-Stimulating Factor 1 Receptor Blockade Inhibits Tumor Growth by Altering the Polarization of Tumor-Associated Macrophages in Hepatocellular Carcinoma | Molecular cancer therapeutics | 28572167 | A human umbilical vein endothelial cell (HUVEC) line was purchased from Allcells and was grown in Allcells completed medium supplemented with 10% FBS. All cell lines obtained Save | Full Paper |
2017 | Zhao, H;Gulesserian, S;Malinao, MC;Ganesan, SK;Song, J;Chang, MS;Williams, MM;Zeng, Z;Mattie, M;Mendelsohn, BA;Stover, DR;Doñate, F; | A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own Demise | Molecular cancer therapeutics | 28522588 | Human hematopoietic stem cells (CD133+, catalog no. ABM026F) isolated from bone marrow and human mature neutrophils (catalog no. PB016) were purchased from ALLCELLS. HSC growth media (StemSpan SFEM II, catalog no. 09600), supplements for expansion (C | Full Paper |
2017 | Tao, B;Wang, R;Sun, C;Zhu, Y; | 3-Mercaptopyruvate Sulfurtransferase, Not Cystathionine β-Synthase Nor Cystathionine γ-Lyase, Mediates Hypoxia-Induced Migration of Vascular Endothelial Cells | Frontiers in pharmacology | 28979207 | et al., 2007). Materials and methods Cell culture Primary human umbilical vein endothelial cells (HUVECs) and culture medium (supplemented with or without 10% fetal bovine serum) were purchased from Allcells (Shanghai, China). Cells were cultured in | Full Paper |
2017 | Hall, BM;Balan, V;Gleiberman, AS;Strom, E;Krasnov, P;Virtuoso, LP;Rydkina, E;Vujcic, S;Balan, K;Gitlin, II;Leonova, KI;Consiglio, CR;Gollnick, SO;Chernova, OB;Gudkov, AV; | p16(Ink4a) and senescence-associated β-galactosidase can be induced in macrophages as part of a reversible response to physiological stimuli | Aging | 28768895 | enmark) where live and dead counts of nucleated cells were quantitated on a NucleoCounter NC-200 (ChemoMetec) via staining with acridine orange and DAPI. Primary human neonatal dermal fibroblasts (AllCells, LLC; Alameda, CA), pooled equally from th | Full Paper |
2017 | Bam, RA;Hansen, D;Irrinki, A;Mulato, A;Jones, GS;Hesselgesser, J;Frey, CR;Cihlar, T;Yant, SR; | TLR7 Agonist GS-9620 Is a Potent Inhibitor of Acute HIV-1 Infection in Human Peripheral Blood Mononuclear Cells | Antimicrobial agents and chemotherapy | 27799218 | e anti-human CD8 antibodies, used for flow cytometry experiments, were purchased from BD Biosciences (San Jose, CA, USA) and stored at 4°C in the dark. Primary cells. Leucopacks were purchased from AllCells (Alameda, CA, USA) and were obtained from | Full Paper |
2017 | Kota, DJ;Prabhakara, KS;Toledano-Furman, N;Bhattarai, D;Chen, Q;DiCarlo, B;Smith, P;Triolo, F;Wenzel, PL;Cox, CS;Olson, SD; | Prostaglandin E2 Indicates Therapeutic Efficacy of Mesenchymal Stem Cells in Experimental Traumatic Brain Injury | Stem cells (Dayton, Ohio) | 28233425 | Donors 3305, 3388 P0 MSC were isolated from commercially available fresh human bone marrow (hBM) aspirates (AllCells, Emeryville, CA, https://www.allcells.com/); MSC from donor TBM-1 were obtained from hBM aspirates under the approved IBC protocol # | Full Paper |
2017 | Diaz, MF;Vaidya, AB;Evans, SM;Lee, HJ;Aertker, BM;Alexander, AJ;Price, KM;Ozuna, JA;Liao, GP;Aroom, KR;Xue, H;Gu, L;Omichi, R;Bedi, S;Olson, SD;Cox, CS;Wenzel, PL; | Biomechanical Forces Promote Immune Regulatory Function of Bone Marrow Mesenchymal Stromal Cells | Stem cells (Dayton, Ohio) | 28181347 | Bone marrow MSCs were derived from whole bone marrow from independent human donors (commercially available by AllCells) (Supporting Information Figure S1A). Briefly, mononuclear cells were enriched in the buffy layer of whole bone marrow by phase sep | Full Paper |
2017 | Garbuzova-Davis, S;Haller, E;Lin, R;Borlongan, CV; | Intravenously Transplanted Human Bone Marrow Endothelial Progenitor Cells Engraft Within Brain Capillaries, Preserve Mitochondrial Morphology, and Display Pinocytotic Activity Toward Blood-Brain Barrier Repair in Ischemic Stroke Rats | Stem cells (Dayton, Ohio) | 28142208 | The isolation of human bone marrow endothelial progenitor cells (hBMEPCs) was performed accordingly to the protocol reported previously [52-54] with modifications. Briefly, for each independent experiment, human bone marrows (AllCells, Almeda, CA, ht | Full Paper |
2017 | Zhang, M;Pan, H;Xu, Y;Wang, X;Qiu, Z;Jiang, L; | Allicin Decreases Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Umbilical Vein Endothelial Cells through Suppression of Mitochondrial Dysfunction and Activation of Nrf2 | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 28456799 | Commercial HUVECs were purchased from Allcells (Shanghai Biological Technology Co., Ltd., China). The cells were seeded in RPMI 1640 medium with 10 % fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 60?µg/mL endothelial cell growth suppleme | Full Paper |
2017 | Deng, H;Song, Z;Xu, H;Deng, X;Zhang, Q;Chen, H;Wang, Y;Qin, Y;Li, Y; | MicroRNA-1185 Promotes Arterial Stiffness though Modulating VCAM-1 and E-Selectin Expression | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 28441665 | The pHUVECs were obtained from AllCells (Shanghai, China) and cultured in complete medium (AllCells, Shanghai, China). The HUVSMCs were purchased from ScienCell (Carlsbad, Save | Full Paper |
2017 | Deng, H;Chu, X;Song, Z;Deng, X;Xu, H;Ye, Y;Li, S;Zhang, Q;Sun, C;Li, Y; | MicroRNA-1185 Induces Endothelial Cell Apoptosis by Targeting UVRAG and KRIT1 | Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology | 28441650 | pHUVEC were purchased from Allcells (Shanghai, China) and maintained in complete medium (Allcells). The human endothelial cells line EA.hy926 cells (ATCC, Manassas, VA, USA) Save | Full Paper |
2017 | Shay, AE;Diwakar, BT;Guan, BJ;Narayan, V;Urban, JF;Prabhu, KS; | IL-4 up-regulates cyclooxygenase-1 expression in macrophages | The Journal of biological chemistry | 28684424 | Frozen human PBMC were purchased from AllCells (Alameda, CA) from donors. Upon arrival, cells were thawed in a 37 °C water and enumerated with trypan blue staining using a hemocytometer to determine cell viability. Cells were transferred to a 50-ml t | Full Paper |
2017 | Li, X;Anderson, M;Collin, D;Muegge, I;Wan, J;Brennan, D;Kugler, S;Terenzio, D;Kennedy, C;Lin, S;Labadia, ME;Cook, B;Hughes, R;Farrow, NA; | Structural studies unravel the active conformation of apo RORγt nuclear receptor and a common inverse agonism of two diverse classes of RORγt inhibitors | The Journal of biological chemistry | 28546429 | Frozen CD4+ T cells (AllCells) were thawed and resuspended in X-VIVO media (Lonza) at a cell density of 1 × 106 cells/ml. Skewing cytokines were added to media at the final concentrations of 30 ng/ml IL-23, 10 ng/ml IL-1β, 10 ng/ml IL-6, 2 ng/ml IL-2 | Full Paper |
2017 | Li, J;Bhattacharya, S;Zhou, J;Phadnis-Moghe, AS;Crawford, RB;Kaminski, NE; | Aryl Hydrocarbon Receptor Activation Suppresses EBF1 and PAX5 and Impairs Human B Lymphopoiesis | Journal of immunology (Baltimore, Md. : 1950) | 28978690 | Fresh human CD34 + HSPCs isolated from cord blood from mixed donors were purchased from AllCells (Emeryville, CA). The in vitro feeder-free HSPC culture was modified based on a Save | Full Paper |
2017 | Eskandarpour, M;Alexander, R;Adamson, P;Calder, VL; | Pharmacological Inhibition of Bromodomain Proteins Suppresses Retinal Inflammatory Disease and Downregulates Retinal Th17 Cells | Journal of immunology (Baltimore, Md. : 1950) | 28039300 | Enriched human PBMC-derived CD4+ T cells were obtained (AllCells; Caltag Medsystems, Buckingham, U.K.) and cultured in X-VIVO 20 and polarized to a Th17 phenotype by addition of recombinant human (rh)IL-6 (30 ng/ml), rhIL-23 (20 ng/ml), rhIL-1b (10 n | Full Paper |
2017 | Frescas, D;Hall, BM;Strom, E;Virtuoso, LP;Gupta, M;Gleiberman, AS;Rydkina, E;Balan, V;Vujcic, S;Chernova, OB;Gudkov, AV; | Murine mesenchymal cells that express elevated levels of the CDK inhibitor p16(Ink4a) in vivo are not necessarily senescent | Cell cycle (Georgetown, Tex.) | 28650766 | Human NDFs were purchased from AllCells and maintained in DMEM supplemented with 10% FBS serum, 100 units/mL of penicillin, 100 µg/mL of streptomycin, and 2 mM L-Glutamine Save | Full Paper |
2017 | Li, S;Ning, H;Ye, Y;Wei, W;Guo, R;Song, Q;Liu, L;Liu, Y;Na, L;Niu, Y;Chu, X;Feng, R;Moustaid-Moussa, N;Li, Y;Sun, C; | Increasing extracellular Ca2+ sensitizes TNF-alpha-induced vascular cell adhesion molecule-1 (VCAM-1) via a TRPC1/ERK1/2/NFκB-dependent pathway in human vascular endothelial cells | Biochimica et biophysica acta. Molecular cell research | 28583863 | Primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Cells were used between passages 3 and 6. Human umbilical vein cell line (EA.hy926) and human leukemic monocyte lymphoma cell line (U937) were obt | Full Paper |
2017 | Wang, H;Gonzalez-Garcia, I;Traba, J;Jain, S;Conteh, S;Shin, DM;Qi, C;Gao, Y;Sun, J;Kang, S;Abbasi, S;Naghashfar, Z;Yoon, J;DuBois, W;Kovalchuk, AL;Sack, MN;Duffy, P;Morse, HC; | ATP-degrading ENPP1 is required for survival (or persistence) of long-lived plasma cells | Scientific reports | 29259245 | The efficiency of reconstitution was verified by FACS analyses of blood for frequencies of B and T cells shortly before mice were immunized 8 wks later. Human bone marrow cells were purchased from Allcells (Alameda, CA) and processed for antibody st | Full Paper |
2017 | Katano, I;Nishime, C;Ito, R;Kamisako, T;Mizusawa, T;Ka, Y;Ogura, T;Suemizu, H;Kawakami, Y;Ito, M;Takahashi, T; | Long-term maintenance of peripheral blood derived human NK cells in a novel human IL-15- transgenic NOG mouse | Scientific reports | 29222435 | iminated twice using a MACS LD column (Miltenyi Biotec). The purity of the isolated human NK cells was usually greater than 95%. We also used human Caucasian PB-derived CD56+ NK cells, purchased from AllCells (Berkeley, CA, USA), in some experiments. | Full Paper |
2017 | Zhang, M;Qiu, L;Zhang, Y;Xu, D;Zheng, JC;Jiang, L; | CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells | Scientific reports | 28811579 | Human umbilical cord vein endothelial cells (HUVECs) (AllCells, Shanghai, China) were cultured for up to 5-7 passages in complete endothelial cell growth medium (H004, AllCells, Save | Full Paper |
2017 | Ma, R;Xie, R;Yu, C;Si, Y;Wu, X;Zhao, L;Yao, Z;Fang, S;Chen, H;Novakovic, V;Gao, C;Kou, J;Bi, Y;Thatte, HS;Yu, B;Yang, S;Zhou, J;Shi, J; | Phosphatidylserine-mediated platelet clearance by endothelium decreases platelet aggregates and procoagulant activity in sepsis | Scientific reports | 28694452 | a50,51. Study was performed with the approval of the Ethics Committee of Harbin Medical University in accordance with the Declaration of Helsinki. Reagents Endothelial cell medium was purchased from AllCells (Emeryville, CA, USA). Bovine serum album | Full Paper |
2017 | Garbuzova-Davis, S;Kurien, C;Thomson, A;Falco, D;Ahmad, S;Staffetti, J;Steiner, G;Abraham, S;James, G;Mahendrasah, A;Sanberg, PR;Borlongan, CV; | Endothelial and Astrocytic Support by Human Bone Marrow Stem Cell Grafts into Symptomatic ALS Mice towards Blood-Spinal Cord Barrier Repair | Scientific reports | 28408761 | t 14 weeks of age and weekly thereafter, mice again underwent behavioral testing until 17 weeks of age. Cell preparation and transplant procedure Cryopreserved human bone marrow CD34+ cells (hBM34+, AllCells, Alameda, CA, USA) were thawed rapidly at | Full Paper |
2017 | Li, XH;Xue, WL;Wang, MJ;Zhou, Y;Zhang, CC;Sun, C;Zhu, L;Liang, K;Chen, Y;Tao, BB;Tan, B;Yu, B;Zhu, YC; | H2S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expression | Scientific reports | 28322298 | Primary human umbilical vein endothelial cells were purchased from Allcells (Shanghai, China) and cultured in endothelial complete medium (ECM; Allcells, China) as described Save | Full Paper |
2017 | Fang, HY;Zeng, HW;Lin, LM;Chen, X;Shen, XN;Fu, P;Lv, C;Liu, Q;Liu, RH;Zhang, WD;Zhao, J; | A network-based method for mechanistic investigation of Shexiang Baoxin Pill's treatment of cardiovascular diseases | Scientific reports | 28272527 | HUVECs were seeded into 60 mm cell culture plates with complete endothelial growth medium (AllCells). After reaching 80% confluence, cells were exposed to SBP extract and SBPpc Save | Full Paper |
2017 | Manser, M;Sater, MR;Schmid, CD;Noreen, F;Murbach, M;Kuster, N;Schuermann, D;Schär, P; | ELF-MF exposure affects the robustness of epigenetic programming during granulopoiesis | Scientific reports | 28266526 | CD34-positive cells isolated from human cord blood of mixed donors were obtained from AllCells (Alabama, United States) and propagated in Stemline II medium expansion medium ( Save | Full Paper |
2017 | Wei, G;Jie, Y;Haibo, L;Chaoneng, W;Dong, H;Jianbing, Z;Junjie, G;Leilei, M;Hongtao, S;Yunzeng, Z;Junbo, G; | Dendritic cells derived exosomes migration to spleen and induction of inflammation are regulated by CCR7 | Scientific reports | 28223684 | ons (6 μm) were prepared using a Lab-Tek tissue processor (Leica). The samples were stained with CD11c/CD4 (Abcam) and DAPI and observed with confocal microscopy. CD4+ T cells were purchased from Allcells (Shanghai, China). To determine whether T ce | Full Paper |
2017 | Rodriguez, C;Kessler, F;Dubey, N;Rosa, V;Fechine, G; | CVD graphene transfer procedure to the surface of stainless steel for stem cell proliferation | Surface and Coatings Technology | Human dental pulp stem cells (DPSC) from a single donor (DPF003, Allcells, USA) were cultured in Dulbecco's modified Eagle's medium (Invitrogen, USA), supplemented with 10% Save | Full Paper | |
2017 | Lee, HJ;Diaz, MF;Ewere, A;Olson, SD;Cox, CS;Wenzel, PL; | Focal adhesion kinase signaling regulates anti-inflammatory function of bone marrow mesenchymal stromal cells induced by biomechanical force | Cellular signalling | 28647573 | Bone marrow MSCs were derived from whole bone marrow from independent human donor (AllCells). Cells were isolated and maintained as described previously [26]. Briefly, enriched Save | Full Paper |
2017 | Killilea, DW;Rohner, F;Ghosh, S;Otoo, GE;Smith, L;Siekmann, JH;King, JC; | Identification of a Hemolysis Threshold That Increases Plasma and Serum Zinc Concentration | The Journal of nutrition | 28490675 | Blood samples from 14 healthy participants (Supplemental Table 1) were purchased from a commercial laboratory (AllCells LLC). The laboratory prescreened donors for lack of Save | Full Paper |
2017 | Bellayr, IH;Kumar, A;Puri, RK; | MicroRNA expression in bone marrow-derived human multipotent Stromal cells | BMC genomics | 28800721 | A total of 8 human bone marrow-derived MSCs from 8 donors were purchased from ALLCELLS and Lonza at passage 1 (Additional file 1: Table S1). By flow cytometry, all MSCs Save | Full Paper |
2017 | Huang, J;Khan, A;Au, BC;Barber, DL;López-Vásquez, L;Prokopishyn, NL;Boutin, M;Rothe, M;Rip, JW;Abaoui, M;Nagree, MS;Dworski, S;Schambach, A;Keating, A;West, ML;Klassen, J;Turner, PV;Sirrs, S;Rupar, CA;Auray-Blais, C;Foley, R;Medin, JA; | Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34+ Cells for Correction of Fabry Disease | Molecular therapy. Methods & clinical development | 28603745 | lutamine (PSQ) (2 mM glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin; Gibco by Life Technologies). Mobilized peripheral blood normal CD34+ cells from healthy donors were purchased from AllCells. Human primary CD34+ cells were cultured in | Full Paper |
2017 | Lawson, T;Kehoe, D;Schnitzler, A;Rapiejko, P;Der, K;Philbrick, K;Punreddy, S;Rigby, S;Smith, R;Feng, Q;Murrell, J;Rook, M; | Process development for expansion of human mesenchymal stromal cells in a 50L single-use stirred tank bioreactor | Biochemical Engineering Journal | PBMCs (AllCells, Alameda, CA) that had been thawed and incubated overnight in 3 mL RPMI medium in ultra-low attachment 6-well plates (Corning) were stained with CFDA-SE (Thermo Fisher Scientific), according to the manufacturer’s recommendations. For | Full Paper | |
2017 | Heng, BC;Gong, T;Wang, S;Lim, LW;Wu, W;Zhang, C; | Decellularized Matrix Derived from Neural Differentiation of Embryonic Stem Cells Enhances the Neurogenic Potential of Dental Follicle Stem Cells | Journal of endodontics | 28231979 | The hESCs (WA01 line) were obtained from Wicell Institute (Madison, WI). Human DFSCs (catalog no. FT002 F, lot no. 020609) were purchased from AllCells LLC (Alameda, CA). Unless mentioned otherwise, all cell culture media and supplements were procure | Full Paper |
2017 | Elwood, F;Witter, DJ;Piesvaux, J;Kraybill, B;Bays, N;Alpert, C;Goldenblatt, P;Qu, Y;Ivanovska, I;Lee, HH;Chiu, CS;Tang, H;Scott, ME;Deshmukh, SV;Zielstorff, M;Byford, A;Chakravarthy, K;Dorosh, L;Rivkin, A;Klappenbach, J;Pan, BS;Kariv, I;Dinsmore, C;Slipetz, D;Dandliker, PJ; | Evaluation of JAK3 Biology in Autoimmune Disease Using a Highly Selective, Irreversible JAK3 Inhibitor | The Journal of pharmacology and experimental therapeutics | 28193636 | Frozen, purified human CD34+ from mobilized blood (AllCells) were seeded at 100,000 cell/ml in Stempro34 complete medium (Thermo Fisher) supplemented with GMCSF at 20 ng/ml (Thermo Fisher), human stem cell factor at 40 ng/ml (Cell Signaling), and IL- | Full Paper |
2017 | Erickson, RI;Schutt, LK;Tarrant, JM;McDowell, M;Liu, L;Johnson, AR;Lewin-Koh, SC;Hedehus, M;Ross, J;Carano, RA;Staflin, K;Zhong, F;Crawford, JJ;Zhong, S;Reif, K;Katewa, A;Wong, H;Young, WB;Dambach, DM;Misner, DL; | Bruton's Tyrosine Kinase Small Molecule Inhibitors Induce a Distinct Pancreatic Toxicity in Rats | The Journal of pharmacology and experimental therapeutics | 27821712 | samples, sex unknown; Prodo Laboratories, Irvine, CA), universal RNA (rat/human; Clontech, Mountain View, CA), and total RNA from pooled isolated rat islet preparations (AllCells, Save | Full Paper |
2017 | Ye, J;Si, J;Cui, Z;Wang, Q;Peng, K;Chen, W;Peng, X;Chen, S; | Surface Modification of Electrospun TPU Nanofiber Scaffold with CNF Particles by Ultrasound-Assisted Technique for Tissue Engineering | Macromolecular Materials and Engineering | The cell viability of the TPU and CNF-adsorbed TPU nanofiber was determined using a MTT assay (3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide). Human umbilical vein endothelial cells (HUVECs, AllCells Biological Technology Co., Ltd., | Full Paper | |
2017 | McBride, JD;Rodriguez-Menocal, L;Guzman, W;Candanedo, A;Garcia-Contreras, M;Badiavas, EV; | Bone Marrow Mesenchymal Stem Cell-Derived CD63+ Exosomes Transport Wnt3a Exteriorly and Enhance Dermal Fibroblast Proliferation, Migration, and Angiogenesis In Vitro | Stem cells and development | 28679315 | BM-MSC "line 2" and "line 3" were obtained from healthy male and female donors, respectively, according to quality control and manufacturing established by AllCells , and isolated Save | Full Paper |
2017 | Setrerrahmane, S;Yu, J;Hao, J;Zheng, H;Xu, H; | Novel production method of innovative antiangiogenic and antitumor small peptides in Escherichia coli | Drug design, development and therapy | 29184391 | om GE Healthcare Life Sciences (Logan, UT, USA) and used for gene expression and protein purification, respectively. Human umbilical vein endothelial cells (HUVECs; primary cells) were purchased from AllCells (Shanghai, People’s Republic of China); a | Full Paper |
2017 | d Johnston, IC;Wagemaker, G; | development of a highly efficient lentiviral gene transfer protocol for hematopoietic stem cells | Book | Bone marrow samples were purchased from AllCells (Alameda, USA). These studies were performed according to established ethical guidelines, and all blood donors gave informed Save | Full Paper | |
2017 | Ito, R;Nagai, D;Igo, N;Okuda, Y;Sekine, K;Ichimura, E;Katano, I;Mizushima, T;Goto, M;Ohnishi, Y;Ito, M;Okamoto, K; | A novel in vivo model for predicting myelotoxicity of chemotherapeutic agents using IL-3/GM-CSF transgenic humanized mice | Toxicology letters | 28947270 | Commercially available human cord blood (CB)-derived CD34+ cells obtained from a single donor were purchased from Lonza (Basel, Switzerland) or Allcells (Alameda, CA, USA). The cells were prepared using a standard protocol for xenotransplantation | Full Paper |
2017 | FUKAHORI, H; | Investigation Regarding the Properties of Protein Kinase Cθ Selective Inhibitor as a Novel T cell Immunosuppressant | Thesis | T cell proliferation assay Human peripheral T cells were purchased from AllCells LLC (Alameda, CA, USA). Cells (1 x 105) suspended in RPMI 1640 containing 10% fetal bovine serum Save | Full Paper | |
2017 | Jin, J;Wang, Y;Xu, Y;Zhou, X;Liu, Y;Li, X;Wang, J; | MicroRNA-144 regulates cancer cell proliferation and cell-cycle transition in acute lymphoblastic leukemia through the interaction of FMN2 | The journal of gene medicine | 27556228 | ALL cell lines, Molt-3, Molt-4, Tall-104, Jurkat, CCRF-CEM, CEM/C1 and CEM/C2, were purchased from American Type Culture Collection (American Type Culture Collection, Manassas, VA, USA). A normal peripheral blood pan-T cell line (BPBPT) was purchased | Full Paper |
2017 | Heck, BE;Park, JJ;Makani, V;Kim, EC;Kim, DH; | PPAR-δ Agonist With Mesenchymal Stem Cells Induces Type II Collagen-Producing Chondrocytes in Human Arthritic Synovial Fluid | Cell transplantation | 28901183 | To eliminate donor-to-donor variability, we used synovial fluid from 3 different OA patients and used BM-MSCs from 3 donors (AllCells) to measure the GAG content. The use of human Save | Full Paper |
2017 | Bender, AT;Gardberg, A;Pereira, A;Johnson, T;Wu, Y;Grenningloh, R;Head, J;Morandi, F;Haselmayer, P;Liu-Bujalski, L; | Ability of Bruton's Tyrosine Kinase Inhibitors to Sequester Y551 and Prevent Phosphorylation Determines Potency for Inhibition of Fc Receptor but not B-Cell Receptor Signaling | Molecular pharmacology | 28062735 | E for 15 minutes and then measures CD63 upregulation by flow cytometry. Human cord blood isolated CD341 stem cells (AllCells, Alameda, CA) were differentiated to mast cells by culture for 8 weeks. Cells were maintained in StemPro-34 media (Gibco, Wal | Full Paper |
2017 | Fujisawa, K;Takami, T;Fukui, Y;Quintanilha, LF;Matsumoto, T;Yamamoto, N;Sakaida, I; | Evaluating effects of L-carnitine on human bone-marrow-derived mesenchymal stem cells | Cell and tissue research | 28197778 | The studied BmMSCs were bone marrow cells purchased from Allcells and were continuously cultured and used at passage 4 and passage 16. Flow cytometry results confirmed that Save | Full Paper |
2017 | Bai, L;Li, Q;Duo, X;Hao, X;Zhang, W;Shi, C;Guo, J;Ren, X;Feng, Y; | Electrospun PCL-PIBMD/SF blend scaffolds with plasmid complexes for endothelial cell proliferation | RSC Advances | 2.6.2. Cell culture.Human umbilical vein endothelial cells (HUVECs) were bought from Allcells Biomart (Shanghai, China) and cultured in Dulbecco's modified Eagle medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), 100 μg | Full Paper | |
2017 | Dong, B;Zhang, X;Fan, Y;Cao, S;Zhang, X; | GPR4 knockout improves renal ischemia-reperfusion injury and inhibits apoptosis via suppressing the expression of CHOP | The Biochemical journal | 29089376 | Cell culture and treatment Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCELLS (Emeryville, CA, U.S.A.). Cells were cultured in Dulbecco's modified Eagle medium (Gibco, U.S.A.) with 10% fetal bovine serum (Gibco, U.S.A.) at 3 | Full Paper |
2017 | Li, S;Yuan, H;Pan, J;Fan, W;Zhu, L;Yan, Z;Guo, C; | The treatment of femoral neck fracture using VEGF-loaded nanographene coated internal fixation screws | PloS one | 29117211 | The human umbilical vein endothelial cells (HUVECs) (Allcells, Alameda, CA, USA) and osteoblasts (Type culture collection of the Chinese Academy of Sciences, Shanghai, China) Save | Full Paper |
2017 | Sewing, S;Roth, AB;Winter, M;Dieckmann, A;Bertinetti-Lapatki, C;Tessier, Y;McGinnis, C;Huber, S;Koller, E;Ploix, C;Reed, JC;Singer, T;Rothfuss, A; | Assessing single-stranded oligonucleotide drug-induced effects in vitro reveals key risk factors for thrombocytopenia | PloS one | 29107969 | 0 sec after the end of the association phase in the binding sensorgram were evaluated to rank ONs. Human bone marrow toxicity assay Human mononucleated cells (hMNCs) were received cryopreserved from AllCells, LLC or STEMCELL Technologies Inc, and we | Full Paper |
2017 | Ross, SL;Sherman, M;McElroy, PL;Lofgren, JA;Moody, G;Baeuerle, PA;Coxon, A;Arvedson, T; | Bispecific T cell engager (BiTE®) antibody constructs can mediate bystander tumor cell killing | PloS one | 28837681 | Pan-T cells were purchased from AllCells. T cells that were not stimulated prior to use in assays are referred to as resting T cells. Activated T cells were generated by two methods: (1) Save | Full Paper |
2017 | Liu, L;Koo, Y;Collins, B;Xu, Z;Sankar, J;Yun, Y; | Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic system | PloS one | 28797069 | The product was purchased from Allcells with the concentration of 5 x 10 8 /ml. PRP was used immediately upon arrival. Micro-Renathane tubing (0.066-in. Save | Full Paper |
2017 | Graci, JD;Michaels, D;Chen, G;Schiralli Lester, GM;Nodder, S;Weetall, M;Karp, GM;Gu, Z;Colacino, JM;Henderson, AJ; | Identification of benzazole compounds that induce HIV-1 transcription | PloS one | 28658263 | Brunswick, NJ (UMDNJ, now part of Rutgers University)[48]. ACH-2 and U1 cells were obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH. Cryopreserved PBMCs were purchased from AllCells, LLC (Alameda, CA). RPMI-1640, DMEM, hygr | Full Paper |
2017 | Salzberg, AC;Harris-Becker, A;Popova, EY;Keasey, N;Loughran, TP;Claxton, DF;Grigoryev, SA; | Genome-wide mapping of histone H3K9me2 in acute myeloid leukemia reveals large chromosomal domains associated with massive gene silencing and sites of genome instability | PloS one | 28301528 | Cryopreserved bone marrow CD34+ cells from normal donors were obtained from Allcells (Alameda, CA). Bone marrow CD34+ cells represent a combination of stem and progenitor Save | Full Paper |
2017 | Li, Y;He, Q;Hu, X;Liu, Y;Cheng, X;Li, X;Deng, F; | Improved performance of collagen scaffolds crosslinked by Traut's reagent and Sulfo-SMCC | Journal of biomaterials science. Polymer edition | 28277011 | The HUVECs used in this study were purchased from Allcells (Shanghai). HUVECs were cultured with endothelial complete medium (Allcells, Shanghai) in an incubator with 5% CO 2 Save | Full Paper |
2017 | Tharinger, H;Rebbapragada, I;Samuel, D;Novikov, N;Nguyen, MH;Jordan, R;Frey, CR;Pflanz, S; | Antibody-dependent and antibody-independent uptake of HBsAg across human leucocyte subsets is similar between individuals with chronic hepatitis B virus infection and healthy donors | Journal of viral hepatitis | 28012213 | Peripheral blood from healthy donors (HD) was purchased from AllCells (Alameda, CA, USA). Informed written consent was obtained from all enrolled study subjects prior to blood draw. Additional details related to subject enrolment can be found in supp | Full Paper |
2017 | Bonar, MM;Tilton, JC; | High sensitivity detection and sorting of infectious human immunodeficiency virus (HIV-1) particles by flow virometry | Virology | 28235684 | This study was conducted according to the principles specified in the Declaration of Helsinki and under local ethical guidelines (Case Western Reserve University Institutional Review Board (IRB)). Normal donor samples were de-identified and obtained | Full Paper |
2017 | Chen, S;Wu, X;Hu, J;Dai, G;Rong, A;Guo, G; | PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cells | Molecular medicine reports | 28677750 | HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China). Cells were cultured in RPMI 1640 medium with 20% FBS, Save | Full Paper |
2017 | DiCarlo, AL;Tamarat, R;Rios, CI;Benderitter, M;Czarniecki, CW;Allio, TC;Macchiarini, F;Maidment, BW;Jourdain, JR; | Cellular Therapies for Treatment of Radiation Injury: Report from a NIH/NIAID and IRSN Workshop | Radiation research | 28605260 | having sources of ready-to-inject cells facilitates research. Although cells can be obtained from various commercial sources (e.g., Lonza, AllCells, Allosource), there is often variability in terms of how those cells grow in culture. There are govern | Full Paper |
2017 | Liu, X;Jiang, Y;Wu, J;Zhang, W;Liang, Y;Jia, L;Yu, J;Jeong, LS;Li, L; | NEDD8-activating enzyme inhibitor, MLN4924 (Pevonedistat) induces NOXA-dependent apoptosis through up-regulation of ATF-4 | Biochemical and biophysical research communications | 28450112 | HUVECs (Human umbilical vein endothelial cells) were obtained from Allcells Biotech Co. (Shanghai, China) and maintained in Dulbecco's Modified Eagle's Medium (GIBCO), containing 10% fetal bovine serum (FBS) and 1% Penicillin-Streptomycin solution, a | Full Paper |
2017 | Pittala, V;Vanella, L;Salerno, L;Di Giacomo, C;Acquaviva, R;Raffaele, M;Romeo, G;Modica, MN;Prezzavento, O;Sorrenti, V; | Novel Caffeic Acid Phenethyl Ester (Cape) Analogues as Inducers of Heme Oxygenase-1 | Current pharmaceutical design | 28190391 | Frozen hMSCs, derived from bone marrow, were purchased from Allcells (Emeryville, CA). After thawing, cells were resuspended in α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 20% heat inactivated fetal bovine serum (FB | Full Paper |
2017 | Chan, RC;Kotner, JS;Chuang, CM;Gaur, A; | Stabilization of pre-optimized multicolor antibody cocktails for flow cytometry applications | Cytometry. Part B, Clinical cytometry | 27001933 | RPMI 1640 medium (Hyclone), containing 10% fetal bovine serum (FBS), 2 mmol/L L-glutamine, and antibiotics. PBMCs used in long-term studies were stored in liquid nitrogen vapor. For some experiments, diseased and normal bone marrow samples were purch | Full Paper |
2017 | Dubey, N;Rajan, SS;Bello, YD;Min, KS;Rosa, V; | Graphene Nanosheets to Improve Physico-Mechanical Properties of Bioactive Calcium Silicate Cements | Materials (Basel, Switzerland) | 28772959 | tion, and Bioactivity The use of human monoradicular premolars and dental pulp stem cells (DPSC) for research was approved by the Institutional Review Board/NUS (Approval Number: NUS 2094). The DPSC (Allcells, Alameda, CA, USA) between passage 3 and | Full Paper |
2017 | Seyfried, TF;Gruber, M;Pawlik, MT;Kasper, S;Mandle, RJ;Hansen, E; | A new approach for fat removal in a discontinuous autotransfusion device-concept and evaluation | Vox sanguinis | 28960338 | Background Fat present during blood salvage in orthopaedic or cardiac surgery can pose a risk of fat embolism and should be eliminated before transfusion. Based on observations of Save | Full Paper |
2017 | Fei, T;Shao, Y;Yan, Z;Zhu, L;Li, S;Pan, J;Guo, C; | The effects of P-gp and CYP450 modulated by rifampicin on the steroid-induced osteonecrosis of the femoral head | Journal of bone and mineral metabolism | 27848009 | MSCs (RBM-006, ALLCELLS) were added to 24-well plates and cultured for three passages. The MSCs were incubated with blank medium, medium supplemented with dexamethasone (10 μmol/l), and dexamethasone (10 μmol/l) plus rifampicin (5 μg/ml), each treatm | Full Paper |
2017 | Mao, M;Zheng, X;Jin, B;Zhang, F;Zhu, L;Cui, L; | Effects of CD44 and E-cadherin overexpression on the proliferation, adhesion and invasion of ovarian cancer cells | Experimental and therapeutic medicine | 29285092 | CD44 is a prognostic indicator of shorter survival time in ovarian cancer. E‑cadherin fragmentation promotes the progression of ovarian cancer. However, the effects of CD44 and E‑ Save | Full Paper |
2017 | Sutou, S; | The 10th anniversary of the publication of genes and environment: memoir of establishing the Japanese environmental mutagen society and a proposal for a new collaborative study on mutagenic hormesis | Genes and environment : the official journal of the Japanese Environmental Mutagen Society | 28265305 | Some chemicals probably are mutagenic or carcinogenic to virtually allcells of all organisms. Among these would be the unstable chemical species such as alkylating agents and other Save | Full Paper |
2017 | He, L;Fan, F;Hou, X;Gao, C;Meng, L;Meng, S;Huang, S;Wu, H; | Resveratrol suppresses pulmonary tumor metastasis by inhibiting platelet-mediated angiogenic responses | The Journal of surgical research | 28629815 | Primary HUVECs were obtained from Allcells (Shanghai, China) and cultured in endothelial basal medium (Allcells) according to the manufacturer's protocol. Save | Full Paper |
2017 | Iwasa, M;Miura, Y;Fujishiro, A;Fujii, S;Sugino, N;Yoshioka, S;Yokota, A;Hishita, T;Hirai, H;Andoh, A;Ichinohe, T;Maekawa, T; | Bortezomib interferes with adhesion of B cell precursor acute lymphoblastic leukemia cells through SPARC up-regulation in human bone marrow mesenchymal stromal/stem cells | International journal of hematology | 28044259 | Human BM-MSCs were isolated from BM samples of healthy adults purchased from AllCells (Emeryville, CA, USA) based on our previously published method. A single-cell suspension Save | Full Paper |
2017 | Suknuntha, K;Thita, T;Togarrati, PP;Ratanachamnong, P;Wongtrakoongate, P;Srihirun, S;Slukvin, I;Hongeng, S; | Wnt signaling inhibitor FH535 selectively inhibits cell proliferation and potentiates imatinib-induced apoptosis in myeloid leukemia cell lines | International journal of hematology | 27766528 | Mononuclear cells from CML patient in chronic phase were procured from AllCells (CA, USA). Histopaque-1077 (Sigma-Aldrich) density gradient centrifugation was performed on total Save | Full Paper |
2017 | Hsieh, YT;Aggarwal, P;Cirelli, D;Gu, L;Surowy, T;Mozier, NM; | Characterization of FcγRIIIA effector cells used in in vitro ADCC bioassay: Comparison of primary NK cells with engineered NK-92 and Jurkat T cells | Journal of immunological methods | 27939300 | HemaCare (HemaCare Corporation, Van Nuys, CA) and AllCells (AllCells, LLC, Alameda, CA), respectively. Engineered NK-92 (V-158) and NK-92 (F-158) effector cell lines were purchased under a license agreement from Conkwest. The parent cells were transf | Full Paper |
2017 | Trang, K;Raposo, RA;Lowe, MM;Krow-Lucal, ER;Yonemoto, W;Cabido, VD;SenGupta, D;McCune, JM; | Relative mRNA Expression Levels of Restriction Factors and Antiviral Genes in Fetal and Adult Human Monocytes and Monocyte-Derived Macrophages | Viral immunology | 28151065 | ABM samples were obtained from healthy donors (AllCells, LLC or Lonza, Inc.). Both adult and fetal mononuclear cells were isolated by density centrifugation of a Ficoll-Hypaque Save | Full Paper |
2017 | Zhou, X;Liang, L;Zhao, Y;Zhang, H; | Epigallocatechin-3-Gallate Ameliorates Angiotensin II-Induced Oxidative Stress and Apoptosis in Human Umbilical Vein Endothelial Cells through the Activation of Nrf2/Caspase-3 Signaling | Journal of vascular research | 28942440 | HUVECs were obtained from Allcells Technology, China. The cells were seeded in RPMI 1640 medium with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 60 μg/mL of endothelial cell growth supplement (BD, San Diego, CA, USA) in an incubator at | Full Paper |
2017 | Goto, K;Goto, M;Ando-Imaoka, M;Kai, K;Mori, K; | Evaluation of drug-induced hematotoxicity using novel in vitro monkey CFU-GM and BFU-E colony assays | The Journal of toxicological sciences | 28717098 | Commercial leukopaks generally contain 80-200 mL of processed material with approximately 7x109peripheral blood mononuclear cells (PBMC), (more information available on: www.allcells.com/products/whole-tissue/leuko-pak). The Table I shows the percent | Full Paper |
2017 | Gao, X;Yourick, JJ;Sprando, RL; | Comparative transcriptomic analysis of endothelial progenitor cells derived from umbilical cord blood and adult peripheral blood: Implications for the generation of induced pluripotent stem cells | Stem cell research | 29156376 | Frozen human MNCs from umbilical CB and adult PB of healthy individuals were obtained from AllCells (Alameda, CA). The cells were thawed, washed, and resuspended in EGM-2 Save | Full Paper |
2017 | Enezei, H;Ahmad, A;Khamis, M;Suzuki, J;Sugita, Y;Maeda, H;Alshehadat, S;Razak, N;Abbas, S;Qabbani, A;Alam, M; | Enhanced Osteogenic and Angiogenic-Related Gene Expression of Human Dental Stem Cells on Biphasic Calcium Phosphate Scaffold Treated with Vascular Endothelial Growth Factor: Part I | Journal of Hard Tissue Biology | Cell culture: Commercially obtained DSCs from (AllCells, LLC Company, Emeryville, CA, USA) were cultured in the Dulbecco's Modifi ed Eagle Medium (DMEM) high glucose, (4.5g/l), Fetal bovine serum (FBS), Dulbecco's PBS (without Ca+2 and Mg+2), Penicil | Full Paper | |
2017 | Jasińska-Stroschein, M;Orszulak-Michalak, D; | Novel strategies for treatment of pulmonary arterial hypertension | Postepy higieny i medycyny doswiadczalnej (Online) | 28791952 | 2.3.2 EARLY OUTGROWTH EPC ISOLATION Human early EPCs were generated from leukapheresis samples obtained from Leuko-Paks purchased from AllCells, inc (Almeda, California, USA) or obtained from healthy donors in accordance with the Ottawa Hospital Rese | Full Paper |
2017 | Cheng, Z;Yi, Y;Xie, S;Yu, H;Peng, H;Zhang, G; | The effect of the JAK2 inhibitor TG101209 against T cell acute lymphoblastic leukemia (T-ALL) is mediated by inhibition of JAK-STAT signaling and activation of the crosstalk between apoptosis and autophagy signaling | Oncotarget | 29290986 | (A) HSD2, peer and bone marrow primary T-ALLcells were treated with increasing concentrations of TG101209 for 48 h, which was followed by analysis of apoptosis by staining with PI Save | Full Paper |
2017 | Chen, C;Li, H;Jiang, J;Zhang, Q;Yan, F; | Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats | Oncotarget | 29069818 | t have structural similarities to the human body to obtain more precise results. MATERIALS AND METHODS Cultivation of BMMSCs Bone marrow mesenchymal stem cell (BMMSCs) of SD rats were purchased from AllCells, LLC (Alameda, CA, USA), isolated and pur | Full Paper |
2017 | Brin, E;Wu, K;Lu, HT;He, Y;Dai, Z;He, W; | PEGylated arginine deiminase can modulate tumor immune microenvironment by affecting immune checkpoint expression, decreasing regulatory T cell accumulation and inducing tumor T cell infiltration | Oncotarget | 28938609 | richia coli. The recombinant protein is PEGylated with 20,000 MW PEG [40-42]. C397A substitution was used to generate the inactive ADI-PEG 20 mutant. Frozen healthy donor PBMCs were purchased from AllCells. Cancer cell lines AGS, A2780, A375, HCT116 | Full Paper |
2017 | Horn, LA;Ciavattone, NG;Atkinson, R;Woldergerima, N;Wolf, J;Clements, VK;Sinha, P;Poudel, M;Ostrand-Rosenberg, S; | CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1+ tumor cells, and extends the survival of tumor-bearing humanized mice | Oncotarget | 28938530 | PBMC used for NKT purification were obtained from AllCells, LLC (Alameda, CA) and were handled according to the manufacturer's direction. Fresh samples of blood from SCLC and Save | Full Paper |
2017 | Bilger, A;Plowshay, J;Ma, S;Nawandar, D;Barlow, EA;Romero-Masters, JC;Bristol, JA;Li, Z;Tsai, MH;Delecluse, HJ;Kenney, SC; | Leflunomide/teriflunomide inhibit Epstein-Barr virus (EBV)- induced lymphoproliferative disease and lytic viral replication | Oncotarget | 28574826 | id cell line (LCL) derived from the peripheral blood leukocytes of anonymous donors, obtained from the American Red Cross [55]. M81-Luc LCLs were obtained by infecting CD34-depleted human cord blood (AllCells) with M81-Luc virus, described below, and | Full Paper |
2017 | Estupina, P;Fontayne, A;Barret, JM;Kersual, N;Dubreuil, O;Le Blay, M;Pichard, A;Jarlier, M;Pugnière, M;Chauvin, M;Chardès, T;Pouget, JP;Deshayes, E;Rossignol, A;Abache, T;de Romeuf, C;Terrier, A;Verhaeghe, L;Gaucher, C;Prost, JF;Pèlegrin, A;Navarro-Teulon, I; | The anti-tumor efficacy of 3C23K, a glyco-engineered humanized anti-MISRII antibody, in an ovarian cancer model is mainly mediated by engagement of immune effector cells | Oncotarget | 28427157 | Ficoll procedure. Human and cynomolgus monkey PBMC were frozen in FBS with 10% DMSO and stored in liquid nitrogen until use. Murine PBMC isolated from C57BL/6 mice were directly purchased frozen from AllCells (Alameda, CA, USA). 51Cr-labeled COV434-M | Full Paper |
2017 | Diaz, MF;Evans, SM;Olson, SD;Cox, CS;Wenzel, PL; | A Co-culture Assay to Determine Efficacy of TNF-α Suppression by Biomechanically Induced Human Bone Marrow Mesenchymal Stem Cells | Bio-protocol | 28932763 | Human bone marrow (BM) MSC (Whole Bone Marrow aspirates) (AllCells, catalog number: ABM001-0) MSCs were isolated from whole bone marrow using a Ficoll gradient followed by plastic adherence and then cultured in MSC media (see Recipes) | Full Paper |
2017 | Noorani, TY;Luddin, N;Rahman, IA;Masudi, SM; | In Vitro Cytotoxicity Evaluation of Novel Nano-Hydroxyapatite-Silica Incorporated Glass Ionomer Cement | Journal of clinical and diagnostic research : JCDR | 28571275 | Mesenchymal stem cell (MSC) Basal Medium and supplements were purchased from ALLCELLS (USA). Tryp LE express was purchased from Invitrogen (California, USA). For MTT test, Save | Full Paper |
2017 | Wang, J;Zhang, Y;Zhu, Q;Liu, Y;Cheng, H;Zhang, Y;Li, T; | Data on the radioprotective effect of emodin in vivo and vitro via inhibition of apoptosis and modulation of p53 | Data in brief | 28275662 | aterials and methods 2.1 Experimental design and materials Male C57BL/6 mice were purchased from SIPPR-BK Experiment Animal Co. (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) from ALLCells, LLC (Emeryville, CA, USA). Emodin (purit | Full Paper |
2017 | Ahmed, H;Luddin, N;Kannan, T;Mokhtar, K;Ahmad, A; | Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cells | European Journal of General Dentistry | molars (adult) and characterized was purchased from AllCells (MD, USA) and was used in (AllCells), with 50 ml of human mesenchymal stem cell stimulatory supplements (AllCells). Save | Full Paper | |
2017 | 张文博, ;张贤, ; | Influence of M2 macrophage supernatant combined with Eucommia flavonoids on the biological behavior of osteoblasts under hypoxic conditions | Chinese Journal of Tissue Engineering Research | : 文 快速阅读: 文题释义: M2 型巨噬细胞: 即替代活化巨噬细胞, 由白细胞介 4, 白细胞介 13 诱导, 可使甘露糖受体及转谷氨酰胺酶2 表达上调. M2 型巨噬细胞及其分泌 子可以促进组织 Save;METHODS:AfterMC3T3E1cellswereresuscitated,andM2macrophageswereisolated,inducedandcultured,allcells weredividedintocontrol,M2macrophage, Save | Full Paper | |
2017 | Richardson, C; | Mechanistic and Therapeutic Studies Related to Anemia of Chronic Disease and Inflammation | University of Virginia | follows: IFNγ 1500U/ml, TNFα 100ng/ml, IL-1β 100ng/ml, IL-6 100ng/ml IL-10 100ng/ml, IL-15 100ng/ml, and LPS 50ug/ml (PeproTech). Purified human CD36+ cells (AllCells LLC, Emeryville, CA) underwent expansion as previously described [73] for 48 hours | Full Paper | |
2017 | Alvey, C; | Engineered Marrow Macrophages For Cancer Therapy: Engorgement, Accumulation, Differentiation, And Acquired Immunity | Thesis | Human Specimens: Fresh human bone marrow was purchased from AllCells (Cat#: ABM001-1) in 3 mL quantities from healthy donors. AllCells received IRB approval from Alpha (Protocol #7000-SOP-046) which includes donor consent. In total, there were 4 diff | Full Paper | |
2017 | Walters, MC;Hongeng, S;Kwiatkowski, JL;Locatelli, F; | Results from the Hgb-207 (Northstar-2) Trial: A Phase 3 Study to Evaluate Safety and Efficacy of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia (TDT) in Patients with Non-β0/β0 Genotypes | Blood | oad : Download full-size image [https://ars.els-cdn.com/content/image/1-s2.0-S000649711981042X-gr1.jpg] DISCLOSURES WALTERS: _bluebird bio:_ Research Funding; _Sangamo Therapeutics:_ Consultancy; _AllCells, Inc:_ Other: Medical Director; _ViaCord | Full Paper | |
2017 | Talano, JA;Moore, TB;Keever-Taylor, CA;Shenoy, S; | Promising Results at 1 Year Follow-up Following Familial Haploidentical (FHI) T-Cell Depleted (TCD) with CD34 Enrichment and T-Cell (CD3) Addback Allogeneic Stem Cell Transplantation in Patients with High-Risk Sickle Cell Disease (SCD) (IND 14359) | Blood | ls-cdn.com/content/image/1-s2.0-S0006497119851182-gr1_lrg.jpg] * Download : Download full-size image [https://ars.els-cdn.com/content/image/1-s2.0-S0006497119851182-gr1.jpg] DISCLOSURES WALTERS: _AllCells, Inc:_ Other: Medical Director; _bluebird | Full Paper | |
2017 | Acharya, S; | Defining Immune Cell Profiles and their Role in Predicting Therapeutic Response in Multiple Myeloma | Thesis | Dana-Farber Cancer Institute. These samples were collected after informed consent in accordance with the Declaration of Helsinki and approved by the institutional review board of the Dana-Farber Cancer Institute. Healthy donor samples were obtained f | Full Paper | |
2017 | Accomando, WP;Wiencke, JK;Houseman, EA; | HousemanEugenePHHSQuantitativeReconstructionLeukocyte. pdf | Genome Biology | blood, that were utilized in this work. Blood was obtained through an institutional review board-approved donor program at AllCells, LLC (Alameda, CA, USA) and research was carried out in accordance with the Helsinki declaration. Samples were de-iden | Full Paper | |
2017 | Zhang, Y;Farhangfar, F;Giblin, L;Wolf, D;Vitelli, F; | Assessment of Differentiation States of Hematopoietic Stem Cells Following in Vitro Culture Using Side and Forward Scatter of Flow Cytometry | World Journal of Research and Review | G-CSF mobilized peripheral HSC (AllCells, LLC. Emeryville, CA) were cultured with CellGro®Stem Cell Expansion Media (CellGenix, Inc., Catalog number: 2001) containing 10ng/ml TPO (Peprotech, Inc. Rocky Hill, NJ. Catalog number: 300-18), 50ng/ml SCF | Full Paper | |
2017 | Gregg, KA; | Novel Lipid A Structures for Adjuvant Discovery and Development | Thesis | 100 IU/mL penicillin, 100 µg/mL streptomycin, 1 mM sodium pyruvate, and 200 mM l-glutamine. THP-1 (ATCC, Manassas, VA), primary mouse splenocytes, and primary human PBMCs (AllCells, LLC, Alameda, CA) were cultured in RPMI 1640 | Full Paper | |
2017 | Edun, FA; | Endothelial cell response to bioactive peptides and stiffened extracellular matrices: an investigation into atherogenic pathways | Thesis | example, samples delivered from AllCells (California, U.S.A.) had markedly different culture adherence and proliferation characteristics (cell doubling time) than HUVECs obtained from ThermoFisher, prompting our repeated purchase of only batch number | Full Paper | |
2017 | Bonar, MM; | Rapid Enumeration, Sorting and Maturation Analysis of Single Viral Particles in HIV-1 Swarms by High-resolution Flow Virometry | Thesis | identified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated by adding additional autologous red blood cells to leukapheresis samples and RosetteSep CD4+ T cell enrichment k | Full Paper | |
2017 | Basiorka, AA; | Changing the pathobiological paradigm in myelodysplastic syndromes: The NLRP3 inflammasome drives the MDS phenotype | Thesis | Normal, heparinized BM aspirates were purchased from Lonza Walkersville or AllCells, LLC. Normal and MDS BM mononuclear cells (BMMNC) were isolated from heparinized BM aspirates using Ficoll-Hypaque Plus gradient centrifugation (GE Healthcare). Recom | Full Paper | |
2017 | Bennett, LF; | Analysis of Stress Erythropoiesis during Inflammation: Stimulation of Toll-Like Receptors Induces Erythrophagocytosis and Activates Stress Erythropoiesis | Thesis | After 24 hours, cells were washed and replated with fresh SEEM. Human bone marrow mononuclear cells were purchased from ReachBio (0300-200) and cultured in SEEM containing human insulin, human holo-transferrin, and human cytokines. Human MNCs culture | Full Paper | |
2017 | Newkirk, SJ;Lee, S;Grandi, FC;Gaysinskaya, V;Rosser, JM;Vanden Berg, N;Hogarth, CA;Marchetto, MCN;Muotri, AR;Griswold, MD;Ye, P;Bortvin, A;Gage, FH;Boeke, JD;An, W; | Intact piRNA pathway prevents L1 mobilization in male meiosis | Proceedings of the National Academy of Sciences of the United States of America | 28630288 | wrote the paper. Reviewers: P.D., Tulane Cancer Center; and P.J.W., University of Pennsylvania. 1Present address: Cancer Biology Program, Stanford University, Stanford, CA 94305. 2Present address: ReachBio LLC, Seattle, WA 98107. 3Present address | Full Paper |
2017 | Zhen, A;Peterson, CW;Carrillo, MA;Reddy, SS;Youn, CS;Lam, BB;Chang, NY;Martin, HA;Rick, JW;Kim, J;Neel, NC;Rezek, VK;Kamata, M;Chen, ISY;Zack, JA;Kiem, HP;Kitchen, SG; | Long-term persistence and function of hematopoietic stem cell-derived chimeric antigen receptor T cells in a nonhuman primate model of HIV/AIDS | PLoS pathogens | 29284044 | Following conditioning, the HSPC product was infused back into the autologous animal. A small aliquot of the infused cell product was plated in Colony Gel Medium (Reach Bio, Seattle, WA) and analyzed as previously described[14,39]. Individual colonie | Full Paper |
2017 | Liu, FD;Pishesha, N;Poon, Z;Kaushik, T;Van Vliet, KJ; | Material Viscoelastic Properties Modulate the Mesenchymal Stem Cell Secretome for Applications in Hematopoietic Recovery | ACS biomaterials science & engineering | 33445371 | Human bone marrow-derived MSCs (MSCs) were purchased from multiple commercial sources (Lonza, RoosterBio, and ReachBio) at what was stated by the vendors to be a low Save | Full Paper |
2017 | Hinerfeld, D;Fuhrman, K;Demirkan, G;DosSantos, G;Geiss, G; | A simple and integrated workflow for deep proteomic and transcriptomic analysis of sorted cell populations | The Journal of Immunology | 1Nanostring Technologies++1Nanostring Technologies++1Nanostring Technologies++2Reachbio Research Labs++1Nanostring Technologies | Full Paper | |
2017 | Min, C;Moore, N;Shearstone, JR;Quayle, SN;Huang, P;van Duzer, JH;Jarpe, MB;Jones, SS;Yang, M; | Selective Inhibitors of Histone Deacetylases 1 and 2 Synergize with Azacitidine in Acute Myeloid Leukemia | PloS one | 28060870 | Specifically, compounds were dissolved in DMSO and added to tubes of methylcellulose-based medium (Reachbio, catalog 1103) containing cytokine formulations to support colony Save;row samples from AML patients were treated with ACY-957 alone, azacit | Full Paper |
2017 | Kudrimoti, M;Curtis, A;Azawi, S;Worden, F;Katz, S;Adkins, D;Bonomi, M;Scott, Z;Elder, J;Sonis, ST;Straube, R;Donini, O; | Dusquetide: Reduction in oral mucositis associated with enduring ancillary benefits in tumor resolution and decreased mortality in head and neck cancer patients | Biotechnology reports (Amsterdam, Netherlands) | 28649557 | 1).Fig. 1Dusquetide Pre-Incubated with Stromal Cells inhibits the Future Growth of Co-cultured Multiple Myeloma Cells. Previously defined primary human bone marrow stromal (mesenchymal (MSC)) cells (ReachBio lot# 2221207) were plated at a concentrati | Full Paper |
2017 | Bennett, LF; | Analysis of Stress Erythropoiesis: Stimulation of Toll-Like Receptors Induces Erythrophagocytosis and Activates Stress Erythropoiesis | Thesis | After 24 hours, cells were washed and replated with fresh SEEM. Human bone marrow mononuclear cells were purchased from ReachBio (0300-200) and cultured in SEEM containing human insulin, human holo-transferrin, and human cytokines. Human MNCs culture | Full Paper | |
2017 | Bogeholz, JL; | CD 33 in der akuten melodischen Leukämie | Thesis | ColonyGel 1101 ReachBio, Seattle, WA Steriles Wasser Helmholtzzentrum Fc-Block (FcR Blocking Reagent) Miltenyi Biotec, Bergisch Gladbach | Full Paper | |
2016 | Goldberg, SB;Gettinger, SN;Mahajan, A;Chiang, AC;Herbst, RS;Sznol, M;Tsiouris, AJ;Cohen, J;Vortmeyer, A;Jilaveanu, L;Yu, J;Hegde, U;Speaker, S;Madura, M;Ralabate, A;Rivera, A;Rowen, E;Gerrish, H;Yao, X;Chiang, V;Kluger, HM; | Pembrolizumab for patients with melanoma or non-small-cell lung cancer and untreated brain metastases: early analysis of a non-randomised, open-label, phase 2 trial | Lancet Oncol. | 27267608 | In patients with NSCLC, PD-L1 staining was done by Qualtek (Goleta, CA, USA) on tissue from any disease site after the most recent systemic therapy employing previously described methods 6 using 1% staining as a cutpoint for positivity | Full Paper |
2016 | Seiwert, TY;Burtness, B;Mehra, R;Weiss, J;Berger, R;Eder, JP;Heath, K;McClanahan, T;Lunceford, J;Gause, C;Cheng, JD;Chow, LQ; | Safety and clinical activity of pembrolizumab for treatment of recurrent or metastatic squamous cell carcinoma of the head and neck (KEYNOTE-012): an open-label, multicentre, phase 1b trial | Lancet Oncol. | 27247226 | Funding for this research was provided by Merck & Co, Inc, Kenilworth, NJ, USA. The authors thank the patients and their families and caregivers for participating in the study. The authors also thank Mark Ayers, Michael Nebozhyn, Erin Murphy, and Jen | Full Paper |
2016 | Muro, K;Chung, HC;Shankaran, V;Geva, R;Catenacci, D;Gupta, S;Eder, JP;Golan, T;Le, DT;Burtness, B;McRee, AJ;Lin, CC;Pathiraja, K;Lunceford, J;Emancipator, K;Juco, J;Koshiji, M;Bang, YJ; | Pembrolizumab for patients with PD-L1-positive advanced gastric cancer (KEYNOTE-012): a multicentre, open-label, phase 1b trial | Lancet Oncol. | 27157491 | To screen for PD-L1 expression at enrolment, archival tumour samples were assessed with a laboratory-developed prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA, USA), which included the 22C3 antibody (Merck & Co, Kenil | Full Paper |
2016 | Snyder, MW;Kircher, M;Hill, AJ;Daza, RM;Shendure, J; | Cell-free DNA Comprises an In Vivo Nucleosome Footprint that Informs Its Tissues-Of-Origin | Cell | 26771485 | ... man peripheral blood plasma from healthy donors, donors with clinical diagnosis of Stage IV cancer, and donors with clinical diagnosis with autoimmune disease (Tables S1, S4 and S5) was obtained from Conversant Bio (Huntsville, Alabama, USA) or P | Full Paper |
2016 | Armand, P;Shipp, MA;Ribrag, V;Michot, JM;Zinzani, PL;Kuruvilla, J;Snyder, ES;Ricart, AD;Balakumaran, A;Rose, S;Moskowitz, CH; | Programmed Death-1 Blockade With Pembrolizumab in Patients With Classical Hodgkin Lymphoma After Brentuximab Vedotin Failure | J. Clin. Oncol. | 27354476 | PD-L1 expression was determined using either fresh or archival formalin-fixed, paraffin-embedded tissue sectioned at 4 to 5 microns, with a proprietary assay developed at QualTek Molecular Laboratories (Newtown, PA) in collaboration with Merck (Kenil | Full Paper |
2016 | Nanda, R;Chow, LQ;Dees, EC;Berger, R;Gupta, S;Geva, R;Pusztai, L;Pathiraja, K;Aktan, G;Cheng, JD;Karantza, V;Buisseret, L; | Pembrolizumab in Patients With Advanced Triple-Negative Breast Cancer: Phase Ib KEYNOTE-012 Study | J. Clin. Oncol. | 27138582 | Filhart and Kenneth Emancipator (Merck & Co., Kenilworth, NJ) for immunohistochemistry expertise and study support, Roger Dansey (Merck & Co.) for critical manuscript review and study support, Karl Heath (Merck & Co.) for study support, and QualTek M | Full Paper |
2016 | Imsland, F;McGowan, K;Rubin, CJ;Henegar, C;Sundström, E;Berglund, J;Schwochow, D;Gustafson, U;Imsland, P;Lindblad-Toh, K;Lindgren, G;Mikko, S;Millon, L;Wade, C;Schubert, M;Orlando, L;Penedo, MC;Barsh, GS;Andersson, L; | Regulatory mutations in TBX3 disrupt asymmetric hair pigmentation that underlies Dun camouflage color in horses | Nat. Genet. | 26691985 | Science for Life Laboratory Uppsala, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, USA.++Science for Life Laboratory Uppsala, Department of Med | Full Paper |
2016 | Wang, G;Lu, X;Dey, P;Deng, P;Wu, CC;Jiang, S;Fang, Z;Zhao, K;Konaparthi, R;Hua, S;Zhang, J;Li-Ning-Tapia, EM;Kapoor, A;Wu, CJ;Patel, NB;Guo, Z;Ramamoorthy, V;Tieu, TN;Heffernan, T;Zhao, D;Shang, X;Khadka, S;Hou, P;Hu, B;Jin, EJ;Yao, W;Pan, X;Ding, Z;Shi, Y;Li, L;Chang, Q;Troncoso, P;Logothetis, CJ;McArthur, MJ;Chin, L;Wang, YA;DePinho, RA; | Targeting YAP-Dependent MDSC Infiltration Impairs Tumor Progression | Cancer Discov | 26701088 | ... d R&D Biosystems (Minneaplois, MN). Cxcr2 antibody was obtained from Bioss and R & D Biosystems. CD45 and Ly-6G antibodies were obtained from Biolegend. Prostate tissue micrcroarray was obtained from Folio Bioscience. CHROMATIN IMMUNOPRECIPITATI | Full Paper |
2016 | Vilgelm, AE;Johnson, CA;Prasad, N;Yang, J;Chen, SC;Ayers, GD;Pawlikowski, JS;Raman, D;Sosman, JA;Kelley, M;Ecsedy, JA;Shyr, Y;Levy, SE;Richmond, A; | Connecting the Dots: Therapy-Induced Senescence and a Tumor-Suppressive Immune Microenvironment | J. Natl. Cancer Inst. | 26719346 | Tennessee Valley Healthcare System, Department of Veterans Affairs, Nashville, TN (AEV, CAJ, JY, JSP, DR, AR); Department of Cancer Biology (AEV, AJ, JY, SCC, JSP, DR, AR), Center for Quantitative Sciences (SCC), Division of Cancer Biostatistics, Dep | Full Paper |
2016 | Sun, JM;Zhou, W;Choi, YL;Choi, SJ;Kim, SE;Wang, Z;Dolled-Filhart, M;Emancipator, K;Wu, D;Weiner, R;Frisman, D;Kim, HK;Choi, YS;Shim, YM;Kim, J; | Prognostic Significance of PD-L1 in Patients with Non-Small Cell Lung Cancer: A Large Cohort Study of Surgically Resected Cases | J Thorac Oncol | 27103510 | Automated staining (QualTek TekMate 500, QualTek Molecular Laboratories, Newtown, PA) included rinses with 1× wash buffer (#K8012 Envision FLEX+ kit, Dako), 10-minute proteinase K incubation (#S3020, Dako), 1× wash buffer rinse, and peroxidase blocki | Full Paper |
2016 | Wakeman, CA;Moore, JL;Noto, MJ;Zhang, Y;Singleton, MD;Prentice, BM;Gilston, BA;Doster, RS;Gaddy, JA;Chazin, WJ;Caprioli, RM;Skaar, EP; | The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interaction | Nat Commun | 27301800 | Total RNA was harvested using a combination of LETS buffer (0.1 M LiCl; 10 mM EDTA; 10 mM Tris HCl pH 7.4; 1% SDS) and TRI Reagent as previously described61. qRT–PCR was performed using SYBR green supermix (Bio-Rad) following manufacturer’s instructi | Full Paper |
2016 | Yang, Z;Shah, K;Khodadadi-Jamayran, A;Jiang, H; | Dpy30 is critical for maintaining the identity and function of adult hematopoietic stem cells | J. Exp. Med. | 27647347 | Total RNAs and ChIP (and their corresponding input) DNAs were sent to the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology (Huntsville, AL) for sequencing, including library construction. In brief, the quality of the total RNA and | Full Paper |
2016 | Resendez, SL;Jennings, JH;Ung, RL;Namboodiri, VM;Zhou, ZC;Otis, JM;Nomura, H;McHenry, JA;Kosyk, O;Stuber, GD; | Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lenses | Nat Protoc | 26914316 | Dust-off compressed gas (Falcon Safety Products, DPSXL) Gelfoam absorbable sponge (Pfizer Injectables, cat. no. 00009-0342-01) 12-well culture plates (Falcon, cat. no. 353043) Skull screws (Glass and Watch Screws, 900 piece set, JT69900) 1/16-inc | Full Paper |
2016 | Amendola, LM;Jarvik, GP;Leo, MC;McLaughlin, HM;Akkari, Y;Amaral, MD;Berg, JS;Biswas, S;Bowling, KM;Conlin, LK;Cooper, GM;Dorschner, MO;Dulik, MC;Ghazani, AA;Ghosh, R;Green, RC;Hart, R;Horton, C;Johnston, JJ;Lebo, MS;Milosavljevic, A;Ou, J;Pak, CM;Patel, RY;Punj, S;Richards, CS;Salama, J;Strande, NT;Yang, Y;Plon, SE;Biesecker, LG;Rehm, HL; | Performance of ACMG-AMP Variant-Interpretation Guidelines among Nine Laboratories in the Clinical Sequencing Exploratory Research Consortium | Am. J. Hum. Genet. | 27181684 | Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA.++Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA. Electronic address: pair@u.washington.edu | Full Paper |
2016 | Green, RC;Goddard, KAB;Jarvik, GP;Amendola, LM;Appelbaum, PS;Berg, JS;Bernhardt, BA;Biesecker, LG;Biswas, S;Blout, CL;Bowling, KM;Brothers, KB;Burke, W;Caga-Anan, CF;Chinnaiyan, AM;Chung, WK;Clayton, EW;Cooper, GM;East, K;Evans, JP;Fullerton, SM;Garraway, LA;Garrett, JR;Gray, SW;Henderson, GE;Hindorff, LA;Holm, IA;Lewis, MH;Hutter, CM;Janne, PA;Joffe, S;Kaufman, D;Knoppers, BM;Koenig, BA;Krantz, ID;Manolio, TA;McCullough, L;McEwen, J;McGuire, A;Muzny, D;Myers, RM;Nickerson, DA;Ou, J;Parsons, DW;Petersen, GM;Plon, SE;Rehm, HL;Roberts, JS;Robinson, D;Salama, JS;Scollon, S;Sharp, RR;Shirts, B;Spinner, NB;Tabor, HK;Tarczy-Hornoch, P;Veenstra, DL;Wagle, N;Weck, K;Wilfond, BS;Wilhelmsen, K;Wolf, SM;Wynn, J;Yu, JH;, ; | Clinical Sequencing Exploratory Research Consortium: Accelerating Evidence-Based Practice of Genomic Medicine | Am. J. Hum. Genet. | 27181682 | Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Medical School, Boston, MA 02115, USA; Partners Personalized Medicine, Boston, MA | Full Paper |
2016 | Lieberman, LA;Zeng, W;Singh, C;Wang, W;Otipoby, KL;Loh, C;Plavina, T;Gorelik, L;Ransohoff, RM;Cahir-McFarland, E; | CD62L is not a reliable biomarker for predicting PML risk in natalizumab-treated R-MS patients | Neurology | 26718566 | Thirty-four frozen PBMC samples were purchased from Conversant Bio (Huntsville, AL) to test the effects of other medical conditions on the % CD62L determination. Twenty-one matched healthy donor samples were collected. Eight samples were collected fr | Full Paper |
2016 | Hu, X;Liu, X;Moisan, J;Wang, Y;Lesch, CA;Spooner, C;Morgan, RW;Zawidzka, EM;Mertz, D;Bousley, D;Majchrzak, K;Kryczek, I;Taylor, C;Van Huis, C;Skalitzky, D;Hurd, A;Aicher, TD;Toogood, PL;Glick, GD;Paulos, CM;Zou, W;Carter, LL; | Synthetic RORγ agonists regulate multiple pathways to enhance antitumor immunity | Oncoimmunology | 28123897 | ... ion. After 5 days, cytokine levels in the media were determined using a luminex panel (R&D Systems). Cells were collected for flow cytometry analysis. PBMCs from cancer patients were purchased from Conversant Bio and activated with anti-CD3/28 b | Full Paper |
2016 | Cataisson, C;Michalowski, AM;Shibuya, K;Ryscavage, A;Klosterman, M;Wright, L;Dubois, W;Liu, F;Zhuang, A;Rodrigues, KB;Hoover, S;Dwyer, J;Simpson, MR;Merlino, G;Yuspa, SH; | MET signaling in keratinocytes activates EGFR and initiates squamous carcinogenesis | Sci Signal | 27330189 | This procedure was performed by a commercial vendor (Phylogeny, Inc.) according to the published method (88). FFPE TMAs from US Biomax were deparaffinized for 5 minutes in xylene, immersed in 100% ethanol for 5 minutes then air-dried. Treatment was w | Full Paper |
2016 | Bryant, SA;Herdy, JR;Amemiya, CT;Smith, JJ; | Characterization of Somatically-Eliminated Genes During Development of the Sea Lamprey (Petromyzon marinus) | Mol. Biol. Evol. | 27288344 | Total RNA was isolated from lamprey embryos using Trizol extraction. RNA quality was assessed on the Agilent 2100 Bioanalyzer (Agilent Technologies) and samples with RNA Integrity Number (RIN) 8 were sent to the HudsonAlpha Genomic Services Lab (Huds | Full Paper |
2016 | Jiang, L;Huang, J;Higgs, BW;Hu, Z;Xiao, Z;Yao, X;Conley, S;Zhong, H;Liu, Z;Brohawn, P;Shen, D;Wu, S;Ge, X;Jiang, Y;Zhao, Y;Lou, Y;Morehouse, C;Zhu, W;Sebastian, Y;Czapiga, M;Oganesyan, V;Fu, H;Niu, Y;Zhang, W;Streicher, K;Tice, D;Zhao, H;Zhu, M;Xu, L;Herbst, R;Su, X;Gu, Y;Li, S;Huang, L;Gu, J;Han, B;Jallal, B;Shen, H;Yao, Y; | Genomic Landscape Survey Identifies SRSF1 as a Key Oncodriver in Small Cell Lung Cancer | PLoS Genet. | 27093186 | ... s >70%; the tumor content in each NAT was< 3%. The Caucasian SCLC patient cohort consisted of 25 FFPE lung tumor tissue specimens with matched normal adjacent tissue pairs, which were purchased from Conversant Biologics, Inc (Huntsville, AL) (S1 | Full Paper |
2016 | Oksenberg, D;Dufu, K;Patel, MP;Chuang, C;Li, Z;Xu, Q;Silva-Garcia, A;Zhou, C;Hutchaleelaha, A;Patskovska, L;Patskovsky, Y;Almo, SC;Sinha, U;Metcalf, BW;Archer, DR; | GBT440 increases haemoglobin oxygen affinity, reduces sickling and prolongs RBC half-life in a murine model of sickle cell disease | Br. J. Haematol. | 27378309 | USA (IRB 88‐034)]. Blood from healthy donors was obtained from the Stanford Blood Center (Stanford, CA, USA) and umbilical cord blood was obtained from Conversant Bio, Inc. (Huntsville, AL, USA). The anti‐coagulant was | Full Paper |
2016 | Kirby, MK;Ramaker, RC;Gertz, J;Davis, NS;Johnston, BE;Oliver, PG;Sexton, KC;Greeno, EW;Christein, JD;Heslin, MJ;Posey, JA;Grizzle, WE;Vickers, SM;Buchsbaum, DJ;Cooper, SJ;Myers, RM; | RNA sequencing of pancreatic adenocarcinoma tumors yields novel expression patterns associated with long-term survival and reveals a role for ANGPTL4 | Mol Oncol | 27282075 | ... wling for critical reading of the manuscript. We thank Dr. Nick Cochran for packaging the _ANGPTL4_ lentiviral vectors. We thank Dr. Shawn Levy, Braden Boone, Angela Jones, and all the members of the HudsonAlpha Genomic Services Lab for providing | Full Paper |
2016 | O'Shannessy, DJ;Smith, MF;Somers, EB;Jackson, SM;Albone, E;Tomkowicz, B;Cheng, X;Park, Y;Fernando, D;Milinichik, A;Kline, B;Fulton, R;Oberoi, P;Nicolaides, NC; | Novel antibody probes for the characterization of endosialin/TEM-1 | Oncotarget | 27494870 | ... btained from various commercial vendors with Institutional Review Board approvals and patient consent and were collected between 2009 and 2011 (Bioreclamation, Westbury, NY; Indivumed, Lewisburg, PA; Folio Biosciences, Columbus, OH; Discovery Lif | Full Paper |
2016 | Ladd, B;Mazzola, AM;Bihani, T;Lai, Z;Bradford, J;Collins, M;Barry, E;Goeppert, AU;Weir, HM;Hearne, K;Renshaw, JG;Mohseni, M;Hurt, E;Jalla, S;Bao, H;Hollingsworth, R;Reimer, C;Zinda, M;Fawell, S;D'Cruz, CM; | Effective combination therapies in preclinical endocrine resistant breast cancer models harboring ER mutations | Oncotarget | 27472462 | ... ired ER mutations. MATERIALS AND METHODS Generation of the CTX model/efficacy studies Blood samples from MBC patients, consented according to the Human Biological Samples Policy, were purchased from Conversant Biologics. Due to patient confident | Full Paper |
2016 | Bradford, JR;Wappett, M;Beran, G;Logie, A;Delpuech, O;Brown, H;Boros, J;Camp, NJ;McEwen, R;Mazzola, AM;D'Cruz, C;Barry, ST; | Whole transcriptome profiling of patient-derived xenograft models as a tool to identify both tumor and stromal specific biomarkers | Oncotarget | 26980748 | ... subcutaneously into either NSG or CB17 SCID mice. The HBXF-079 sample was implanted orthotopically. The CTC-174 model was developed from circulating tumor cells in patient blood samples procured from Conversant Biologics (Huntsville, AL) and cons | Full Paper |
2016 | Malek, E;Driscoll, JJ; | High throughput chemical library screening identifies a novel p110-δ inhibitor that potentiates the anti-myeloma effect of bortezomib | Oncotarget | ... om Selleck Chemicals (Houston, TX). Human bone marrow stromal cell line HS-5 (ATCC) was cultured in DMEM. Patient bone marrow aspirates were obtained after Institutional Review Board approval or from ConversantBio (Huntsville, AL) and CD138+ cell | Full Paper | |
2016 | Aeffner, F;Martin, NT;Peljto, M;Black, JC;Major, JK;Jangani, M;Ports, MO;Krueger, JS;Young, GD; | Quantitative assessment of pancreatic cancer precursor lesions in IHC-stained tissue with a tissue image analysis platform | Lab. Invest. | 27775692 | tissue was evaluated. Materials and methods. Human PDAC Tissue. IRB-approved, formalin-fixed tumor samples from pancreatic cancer patients were provided by Conversant Bio (Huntsville, AL, USA). Animals. Kras LSL-G12D | Full Paper |
2016 | Haskett, S;Ding, J;Zhang, W;Thai, A;Cullen, P;Xu, S;Petersen, B;Kuznetsov, G;Jandreski, L;Hamann, S;Reynolds, TL;Allaire, N;Zheng, TS;Mingueneau, M; | Identification of Novel CD4+ T Cell Subsets in the Target Tissue of Sjögren's Syndrome and Their Differential Regulation by the Lymphotoxin/LIGHT Signaling Axis | J. Immunol. | 27815440 | Blocks of formalin-fixed tissue biopsies were acquired from Folio Biosciences (Powell, OH). Salivary tissues included submandibular glands (n = 2), parotid glands (n = 1), and labial glands (n = 3) from Sjo¨gren’s patients. Diagnoses were consistent | Full Paper |
2016 | Yoder, KE;Bundschuh, R; | Host Double Strand Break Repair Generates HIV-1 Strains Resistant to CRISPR/Cas9 | Sci Rep | 27404981 | HIV-1 infections were continued for 21 days in 4 replicates per cell type and virus strain. The virus supernatant was passaged to uninfected CRISPR cells. Infection was visually confirmed by the presence of syncytia. Two weeks later the genomic DNA w | Full Paper |
2016 | Dolled-Filhart, M;Locke, D;Murphy, T;Lynch, F;Yearley, JH;Frisman, D;Pierce, R;Weiner, R;Wu, D;Emancipator, K; | Development of a Prototype Immunohistochemistry Assay to Measure Programmed Death Ligand-1 Expression in Tumor Tissue | Arch. Pathol. Lab. Med. | 27788043 | Merck & Co, Inc, Kenilworth, New Jersey; the Departments of Early Clinical & Translational Research, Clinical Histochemistry (Dr Locke), Research and Clinical Client Services (Dr Murphy), and Operations and Client Services (Dr Lynch), QualTek Molecul | Full Paper |
2016 | Dolled-Filhart, M;Roach, C;Toland, G;Stanforth, D;Jansson, M;Lubiniecki, GM;Ponto, G;Emancipator, K; | Development of a Companion Diagnostic for Pembrolizumab in Non-Small Cell Lung Cancer Using Immunohistochemistry for Programmed Death Ligand-1 | Arch. Pathol. Lab. Med. | 27552095 | The prototype PD-L1 IHC assay was used to determine enrollment of patients in KEYNOTE-001 and was performed at a single laboratory site at QualTek (Goleta, California), which is a College of American Pathologists/Clinical Laboratory Improvement Amend | Full Paper |
2016 | Durning, SP;Flanagan-Steet, H;Prasad, N;Wells, L; | O-Linked β-N-acetylglucosamine (O-GlcNAc) Acts as a Glucose Sensor to Epigenetically Regulate the Insulin Gene in Pancreatic Beta Cells | J. Biol. Chem. | 26598517 | samples after the previously described 1-h incubation. Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL) for RNA-Seq library prep and sequencing. Briefly, the concentration and integrity of the | Full Paper |
2016 | VonDran, M;Thomas, JA;Freund, MP;Ritsick, M;Orr, M;Kaye, WE;Bakker, A;Knight, P; | The National Disease Research Interchange and Collaborators on: What Are the Major Hurdles to the Recovery of Human Tissue to Advance Research? | Biopreserv Biobank | 27845557 | The Foundation has collected more than 100 cutaneous neurofibromas along with patient-reported NF medical histories. Both tissue and data are stored at Precision Bioservices (Frederick, MD). Full “omic” characterization of 50 samples was outsourced t | Full Paper |
2016 | Chisamore, MJ;Gentile, MA;Dillon, GM;Baran, M;Gambone, C;Riley, S;Schmidt, A;Flores, O;Wilkinson, H;Alves, SE; | A novel selective androgen receptor modulator (SARM) MK-4541 exerts anti-androgenic activity in the prostate cancer xenograft R-3327G and anabolic activity on skeletal muscle mass & function in castrated mice | J. Steroid Biochem. Mol. Biol. | 27106747 | Excised tumor samples were immediately fixed with 10% buffered formalin, and sent to QualTek (Santa Barbara, CA) where tumors were embedded in paraffin, 4-μm thick sections were mounted on charged slides, dried at 60 °C for one hour, and deparaffinze | Full Paper |
2016 | Qadota, H;Mayans, O;Matsunaga, Y;McMurry, JL;Wilson, KJ;Kwon, GE;Stanford, R;Deehan, K;Tinley, TL;Ngwa, VM;Benian, GM; | The SH3 domain of UNC-89 (obscurin) interacts with paramyosin, a coiled-coil protein, in Caenorhabditis elegans muscle | Mol. Biol. Cell | 27009202 | Genomic DNA from each strain was prepared and provided to the Emory Integrated Genomics Core (EIGC) for Illumina sequencing. The Genomic Services Laboratory at the HudsonAlpha Institute for Biotechnology (Huntsville, AL) performed quality control and | Full Paper |
2016 | Slamecka, J;Salimova, L;McClellan, S;van Kelle, M;Kehl, D;Laurini, J;Cinelli, P;Owen, L;Hoerstrup, SP;Weber, B; | Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions | Cell Cycle | 26654216 | RNA was extracted from iPSC cultures using RNeasy Plus Mini kit (74134, Qiagen, https://www.qiagen.com/us/shop/sample-technologies/rna-sample-technologies/total-rna/rneasy-plus-micro-and-mini-kits). Whole genome expression analysis was performed by t | Full Paper |
2016 | Aycock, KI;Campbell, RL;Lynch, FC;Manning, KB;Craven, BA; | The Importance of Hemorheology and Patient Anatomy on the Hemodynamics in the Inferior Vena Cava | Ann Biomed Eng | 27272211 | Full Paper | |
2016 | Rahman, KM;Camp, ME;Prasad, N;McNeel, AK;Levy, SE;Bartol, FF;Bagnell, CA; | Age and Nursing Affect the Neonatal Porcine Uterine Transcriptome | Biol. Reprod. | 26632611 | United States Department of Agriculture, Agriculture Research Service, Clay Center, Nebraska Department of Animal Sciences, Endocrinology and Animal Biosciences Program, Rutgers University, New Brunswick, New Jersey.++Department of Animal Sciences, E | Full Paper |
2016 | Havird, JC;Santos, SR; | Developmental Transcriptomics of the Hawaiian Anchialine Shrimp Halocaridina rubra Holthuis, 1963 (Crustacea: Atyidae) | Integr. Comp. Biol. | 27400978 | RNA fragmentation, first-strand cDNA synthesis and amplification, adapter and barcode addition, and size selection were then performed according to Meyer et al. (2011) using the step-by-step protocol at http://people.oregonstate.edu/∼meyere/tools.htm | Full Paper |
2016 | Sorensen, SF;Zhou, W;Dolled-Filhart, M;Georgsen, JB;Wang, Z;Emancipator, K;Wu, D;Busch-Sørensen, M;Meldgaard, P;Hager, H; | PD-L1 Expression and Survival among Patients with Advanced Non-Small Cell Lung Cancer Treated with Chemotherapy | Transl Oncol | 26947883 | gen retrieval, along with a 10-minute proteinase K incubation (#S3020, Dako) following a 1 × wash buffer rinse (#K8012 Envision FLEX + kit, Dako) as part of automated staining. Automated staining (QualTek TekMate 500, Newtown, PA) was followed by per | Full Paper |
2016 | Zea, L;Prasad, N;Levy, SE;Stodieck, L;Jones, A;Shrestha, S;Klaus, D; | A Molecular Genetic Basis Explaining Altered Bacterial Behavior in Space | PLoS ONE | 27806055 | BioServe Space Technologies, Aerospace Engineering Sciences Dept., University of Colorado, Boulder, CO, United States of America.++Genomic Services Laboratory, HudsonAlpha Institute for Biotechnology, Huntsville, AL, United States of America.++Genomi | Full Paper |
2016 | McCoy, CR;Rana, S;Stringfellow, SA;Day, JJ;Wyss, JM;Clinton, SM;Kerman, IA; | Neonatal maternal separation stress elicits lasting DNA methylation changes in the hippocampus of stress-reactive Wistar Kyoto rats | Eur. J. Neurosci. | 27643783 | ; Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) for next‐generation sequencing using NEBNext reagents (New England Biolabs, Ipswich, MA) according to manufacturer's recommendations | Full Paper |
2016 | Beck, BH;Fuller, SA;Li, C;Green, BW;Zhao, H;Rawles, SD;Webster, CD;Peatman, E; | Hepatic transcriptomic and metabolic responses of hybrid striped bass (Morone saxatilis×Morone chrysops) to acute and chronic hypoxic insult | Comp. Biochem. Physiol. Part D Genomics Proteomics | 26851735 | treatment) were used for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25 | Full Paper |
2016 | Póvoa, TF;Oliveira, ER;Basílio-de-Oliveira, CA;Nuovo, GJ;Chagas, VL;Salomão, NG;de Barcelos Alves, AM;Mota, EM;Paes, MV; | Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells | PLoS ONE | 28006034 | Janeiro, Brazil. ⨯. Gerard J. Nuovo, Affiliations Ohio State University Comprehensive Cancer Center, Columbus, Ohio, United States of America, Phylogeny Inc, Powell, Ohio, United States of America. ⨯. Vera LA Chagas, Affiliation | Full Paper |
2016 | Stavropoulos, SW;Chen, JX;Sing, RF;Elmasri, F;Silver, MJ;Powell, A;Lynch, FC;Abdel Aal, AK;Lansky, A;Muhs, BE;, ; | Analysis of the Final DENALI Trial Data: A Prospective, Multicenter Study of the Denali Inferior Vena Cava Filter | J Vasc Interv Radiol | 27569678 | Full Paper | |
2016 | Gardet, A;Chou, WC;Reynolds, TL;Velez, DB;Fu, K;Czerkowicz, JM;Bajko, J;Ranger, AM;Allaire, N;Kerns, HM;Ryan, S;Legault, HM;Dunstan, RW;Lafyatis, R;Lukashev, M;Viney, JL;Browning, JL;Rabah, D; | Pristane-Accelerated Autoimmune Disease in (SWR X NZB) F1 Mice Leads to Prominent Tubulointerstitial Inflammation and Human Lupus Nephritis-Like Fibrosis | PLoS ONE | 27760209 | AZ, USA). Needle biopsies from 5 patients with LN (Class II, 1 patient; Class III, 1 patient; Class IV, 1 patient; Class IV/V, 2 patients) were obtained from Folio Biosciences (Powell, OH) and stained using the same methods. For | Full Paper |
2016 | Shearstone, JR;Golonzhka, O;Chonkar, A;Tamang, D;van Duzer, JH;Jones, SS;Jarpe, MB; | Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2 | PLoS ONE | 27073918 | CS2 differentiation media is same as CS2 expansion media, except 3 U/mL EPO. Peripheral blood from patients homozygous for the sickle cell mutation (Conversant Bio) were collected in heparinized tubes and shipped overnight on cool packs | Full Paper |
2016 | Shimizu, T;Seto, T;Hirai, F;Takenoyama, M;Nosaki, K;Tsurutani, J;Kaneda, H;Iwasa, T;Kawakami, H;Noguchi, K;Shimamoto, T;Nakagawa, K; | Phase 1 study of pembrolizumab (MK-3475; anti-PD-1 monoclonal antibody) in Japanese patients with advanced solid tumors | Invest New Drugs | 27000274 | on the confirmation test. PD-L1 expression. PD-L1 expression was measured using immunohistochemistry performed on formalin-fixed, paraffin-embedded tissue sections at QualTek Clinical Laboratories. PD-L1 positivity was ;or postdose sample was po | Full Paper |
2016 | Meaburn, KJ;Agunloye, O;Devine, M;Leshner, M;Roloff, GW;True, LD;Misteli, T; | Tissue-of-origin-specific gene repositioning in breast and prostate cancer | Histochem. Cell Biol. | 26791532 | Tissue sections and tissue microarrays (TMAs) were purchased from US Biomax Inc, Imgenex Corporation, Folio Bioscience and Biochain Institute or were acquired from the University of Washington under the guidelines and approval of the Institutional Re | Full Paper |
2016 | Quadrini, KJ;Hegelund, AC;Cortes, KE;Xue, C;Kennelly, SM;Ji, H;Högerkorp, CM;Mc Closkey, TW; | Validation of a flow cytometry-based assay to assess C5aR receptor occupancy on neutrophils and monocytes for use in drug development | Cytometry B Clin Cytom | 26084468 | a specific Disease Activity Score 28‐joint counts (DAS28) criteria of C‐reactive protein (CRP) ≥ 3.2 and ≥ 2 swollen joints 10, 11 was drawn into K 2 ‐EDTA phlebotomy tubes and stored at 2-8°C. RA patient samples were obtained from Conversant Biologi | Full Paper |
2016 | Gebauer, A;Schmidt, S;Hoffmann, W; | Status and perspective of lab-on-a-chip systems for common diseases: a systematic review from 2003 to 2013 | Per Med | 29749869 | Diagnosis, monitoring Laboratory Patients with advanced-stage breast cancer 73/73 patients 5/5 negative control Conversant Bio (USA), bio options (USA) ? 61 Prospective experimental study Diagnosis, monitoring ? Patients with metastatic lung (n = 55) | Full Paper |
2016 | Shelton, D;Quinlan, A; | Rapid, Sensitive Cancer Biomarker Screening in cfDNA | Genetic Engineering & Biotechnology News | Untested plasma samples from patients who had previously been classified as KRAS mutation positive or negative by FFPE genotyping were purchased from Conversant Bio and ProMedDx. Figure 3. Multiplex detection of KRAS mutations in patient cell-free DN | Full Paper | |
2016 | Justice, JL;Weese, DA;Santos, SR; | Phylogenetic utility, and variability in structure and content, of complete mitochondrial genomes among genetic lineages of the Hawaiian anchialine shrimp Halocaridina rubra Holthuis 1963 (Atyidae:Decapoda) | Mitochondrial DNA A DNA Mapp Seq Anal | 26061341 | The Atyidae are caridean shrimp possessing hair-like setae on their claws and are important contributors to ecological services in tropical and temperate fresh and brackish water ecosystems. Complete mitochondrial genomes have only been reported from | Full Paper |
2016 | Li, S;Garrett-Bakelman, FE;Chung, SS;Sanders, MA;Hricik, T;Rapaport, F;Patel, J;Dillon, R;Vijay, P;Brown, AL;Perl, AE;Cannon, J;Bullinger, L;Luger, S;Becker, M;Lewis, ID;To, LB;Delwel, R;Löwenberg, B;Döhner, H;Döhner, K;Guzman, ML;Hassane, DC;Roboz, GJ;Grimwade, D;Valk, PJ;D'Andrea, RJ;Carroll, M;Park, CY;Neuberg, D;Levine, R;Melnick, AM;Mason, CE; | Distinct evolution and dynamics of epigenetic and genetic heterogeneity in acute myeloid leukemia | Nature medicine | 27322744 | rough AML_101, DNA and RNA were extracted from mononuclear cell layers using standard techniques. Normal bone marrow controls (NBM: CD34+ mononuclear cells; 7 males and 7 females) were purchased from AllCells (Alameda, CA, USA; n = 5) or isolated usi | Full Paper |
2016 | Dever, DP;Bak, RO;Reinisch, A;Camarena, J;Washington, G;Nicolas, CE;Pavel-Dinu, M;Saxena, N;Wilkens, AB;Mantri, S;Uchida, N;Hendel, A;Narla, A;Majeti, R;Weinberg, KI;Porteus, MH; | CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cells | Nature | 27820943 | blood were purchased from AllCells and thawed according to manufacturer's instructions. CD34 + HSPCs from cord blood were either purchased frozen from AllCells or acquired from Save | Full Paper |
2016 | Briney, B;Sok, D;Jardine, JG;Kulp, DW;Skog, P;Menis, S;Jacak, R;Kalyuzhniy, O;de Val, N;Sesterhenn, F;Le, KM;Ramos, A;Jones, M;Saye-Francisco, KL;Blane, TR;Spencer, S;Georgeson, E;Hu, X;Ozorowski, G;Adachi, Y;Kubitz, M;Sarkar, A;Wilson, IA;Ward, AB;Nemazee, D;Burton, DR;Schief, WR; | Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing Antibodies | Cell | 27610570 | pathogen free environment. Healthy, HIV-Negative Human PBMCs Leukopaks from two heathy, HIV-negative individuals (a 28 year-old female and a 33 year-old male) were obtained from a commercial vendor (AllCells) using a protocol approved by the Institu | Full Paper |
2016 | Corces, MR;Buenrostro, JD;Wu, B;Greenside, PG;Chan, SM;Koenig, JL;Snyder, MP;Pritchard, JK;Kundaje, A;Greenleaf, WJ;Majeti, R;Chang, HY; | Lineage-specific and single-cell chromatin accessibility charts human hematopoiesis and leukemia evolution | Nature genetics | 27526324 | Normal donor human bone marrow and peripheral blood cells were obtained fresh from AllCells or the Stanford Blood Center. All normal blood cell populations were sorted fresh. Save | Full Paper |
2016 | Kim, JH;Hu, Y;Yongqing, T;Kim, J;Hughes, VA;Le Nours, J;Marquez, EA;Purcell, AW;Wan, Q;Sugita, M;Rossjohn, J;Winau, F; | CD1a on Langerhans cells controls inflammatory skin disease | Nature immunology | 27548435 | lysis by ELISA. K562 cells were routinely tested for mycoplasma contamination, and CD1a expression of transfectants was confirmed before use by flow cytometry. PBMCs were obtained from ConversantBio, ALLCELLS, and the Kraft Family Blood Donor Center | Full Paper |
2016 | Locci, M;Wu, JE;Arumemi, F;Mikulski, Z;Dahlberg, C;Miller, AT;Crotty, S; | Activin A programs the differentiation of human TFH cells | Nature immunology | 27376469 | Leukapheresis sample from a healthy donor was obtained from AllCells, Inc., after a prescreening of multiple donors. Fresh whole blood samples from healthy donors were obtained Save | Full Paper |
2016 | Wang, X;Haylock, D;Hu, CS;Kowalczyk, W;Jiang, T;Qiu, J;Mosoyan, G;He, W;Marshall, N;Mascarenhas, J;Tarasova, A;Brody, J;Winkler, D;Hoffman, R; | A thrombopoietin receptor antagonist is capable of depleting myelofibrosis hematopoietic stem and progenitor cells | Blood | 27114459 | Flow Cytometer (Becton Dickinson, Franklin Lakes, NJ) were used in each experiment. BM CD34+ cells from normal individuals were purchased from AllCells (Alameda, CA). The JAK2, CALR, and MPL mutational status1,22-24 of each patient is shown in Table | Full Paper |
2016 | Zhang, Y;Quarmyne, M;Himburg, H;Yan, X;McBride, W;Jung, M;Chute, J; | A Small Molecule Inhibitor of Protein Tyrosine Phosphatase-Sigma (PTPσ) Promotes Hematopoietic Stem Cell (HSC) Regeneration | Blood | Our laboratory previously discovered that pleiotrophin, a paracrine growth factor secreted by the BM perivascular and endothelial cells, promoted HSC self-renewal and regeneration Save | Full Paper | |
2016 | Iancu-Rubin, C;Fong, H;Mosoyan, G;Patel, A;Sabado, R;Marcia, M;Moshier, E;Ozbek, U;Keyzner, A;Bhardwaj, N;McNiece, I;Tong, J;Hoffman, R; | Preclinical Development of a Cord Blood (CB)-Derived Hematopoietic Stem Cell (HSC) Product for Allogeneic Transplantation in Patients with Hematological Malignancies | Blood | Division of Hematology and Medical Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY++AllCells, LLC, Alameda, CA++Division of Hematology and Medical Oncology, The Tisch Cancer Institute, Icahn School of Medic | Full Paper | |
2016 | Hoff, F;Hu, C;Qiu, Y;Yoo, S;Qutub, A;Horton, T;Kornblau, S; | Recurrent Patterns of Protein Expression Signatures in Pediatric Acute Lymphoblastic Leukemia: Recognition and Therapeutic Guidance | Blood | reverse-phase protein arrays (RPPA) methodology was performed on 73 samples from pediatric patients with ALL, 10 cryopreserved CD34 + normal bone marrow samples (AllCells) Save | Full Paper | |
2016 | Yoshioka, S;Miura, Y;Iwasa, M;Fujishiro, A;Sugino, N;Fujii, S;Nakagawa, Y;Sato, A;Yokota, A;Hirai, H;Ichinohe, T;Takaori, A;Maekawa, T; | Late Adherent Subpopulation in Umbilical Cord Blood Has the Same Characteristics and Hematopoiesis-Supporting Capacity As Mesenchymal Stromal/Stem Cells | Blood | Mesenchymal stromal/stem cells (MSCs) are a major source of cell for cell therapy. MSCs derived from bone marrow (BMMSCs) have been mostly used in clinical applications. BMMSCs can be easily isolated as a cell population that adheres to plastic cultu | Full Paper | |
2016 | Merkel, P;Giclas, H;Gibula, A;Andersen, G;Knight, V;Lin, J;Castro, G;Green, J;Stassinopoulos, A; | Reinvestigation of the T-Cell Inactivation Achieved for the Prevention of TA GV HD By 2500 Gy of Gamma Irradiation, a Comparison with Pathogen Reduction | Blood | Background: Transfusion Associated Graft vs. Host Disease (TA GVHD) results in high morbidity and mortality, caused by contaminating T-cells present in transfused blood products. Gamma irradiation (GI) of platelet components (PC) with a dose of 2500 | Full Paper | |
2016 | Kanter, J;Walters, M;Hsieh, M;Krishnamurti, L;Kwiatkowski, J;Kamble, R;von Kalle, C;Kuypers, F;Cavazzana, M;Leboulch, P;Joseney-Antoine, M;Asmal, M;Thompson, A;Tisdale, J; | Interim Results from a Phase 1/2 Clinical Study of Lentiglobin Gene Therapy for Severe Sickle Cell Disease | Blood | β-globin gene transfer into hematopoietic stem cells (HSCs) has the potential to reduce or eliminate the symptoms and long-term complications of severe sickle cell disease (SCD). LentiGlobin Drug Product (DP) is a gene therapy product containing auto | Full Paper | |
2016 | Thompson, A;Kwiatkowski, J;Rasko, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;von Kalle, C;Kletzel, M;Leboulch, P;Vichinsky, E;Petrusich, A;Asmal, M;Walters, M; | Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia: Update from the Northstar Hgb-204 Phase 1/2 Clinical Study | Blood | BACKGROUND Allogeneic hematopoietic stem cell (HSC) transplant is potentially curative for patients with β-thalassemia major or, as more broadly defined, transfusion dependent β-thalassemia (TDT). However, HSC transplant is generally r | Full Paper | |
2016 | Crews, LA;Balaian, L;Delos Santos, NP;Leu, HS;Court, AC;Lazzari, E;Sadarangani, A;Zipeto, MA;La Clair, JJ;Villa, R;Kulidjian, A;Storb, R;Morris, SR;Ball, ED;Burkart, MD;Jamieson, CHM; | RNA Splicing Modulation Selectively Impairs Leukemia Stem Cell Maintenance in Secondary Human AML | Cell stem cell | 27570067 | Age-related human hematopoietic stem cell (HSC) exhaustion and myeloid-lineage skewing promote oncogenic transformation of hematopoietic progenitor cells into therapy-resistant Save | Full Paper |
2016 | Song, C;Zhang, Y;Li, C;Chen, G;Kang, X;Wang, Q; | Enhanced Nanodrug Delivery to Solid Tumors Based on a Tumor Vasculature-Targeted Strategy | Advanced Functional Materials | Cell Culture: Human malignantglioma U87-MG cells were obtained from The Cell Bank of Type Culture Collection of Chinese Academy of Sciences and HUVECs were purchased from Allcells (China). U87-MG cells were cultured in low-glucose Dulbecco's modified | Full Paper | |
2016 | Sherbenou, DW;Aftab, BT;Su, Y;Behrens, CR;Wiita, A;Logan, AC;Acosta-Alvear, D;Hann, BC;Walter, P;Shuman, MA;Wu, X;Atkinson, JP;Wolf, JL;Martin, TG;Liu, B; | Antibody-drug conjugate targeting CD46 eliminates multiple myeloma cells | The Journal of clinical investigation | 27841764 | bead columns (Stem Cell Technologies). Primary cells were cultured in RPMI1640 media with 100 U/ml penicillin, 100 μg/ml streptomycin, 10% FBS, and 2 ng/ml IL-6. BM and PB controls from normal donors were purchased from AllCells | Full Paper |
2016 | Jiang, X;Hu, C;Arnovitz, S;Bugno, J;Yu, M;Zuo, Z;Chen, P;Huang, H;Ulrich, B;Gurbuxani, S;Weng, H;Strong, J;Wang, Y;Li, Y;Salat, J;Li, S;Elkahloun, AG;Yang, Y;Neilly, MB;Larson, RA;Le Beau, MM;Herold, T;Bohlander, SK;Liu, PP;Zhang, J;Li, Z;He, C;Jin, J;Hong, S;Chen, J; | miR-22 has a potent anti-tumour role with therapeutic potential in acute myeloid leukaemia | Nature communications | 27116251 | (Axis-Shield, Oslo, Norway) according to the manufacturer’s manual. Human normal hematopoietic control cell samples The MNC normal control samples were isolated from normal BM cells purchased from AllCells, LLC (Emeryville, CA) by use of NycoPrep 1. | Full Paper |
2016 | van Overbeek, M;Capurso, D;Carter, MM;Thompson, MS;Frias, E;Russ, C;Reece-Hoyes, JS;Nye, C;Gradia, S;Vidal, B;Zheng, J;Hoffman, GR;Fuller, CK;May, AP; | DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated Breaks | Molecular cell | 27499295 | Mobilized peripheral blood HSCs (AllCells # mPB018F) were plated at 1.5 × 105/ml in DMEM/F12 Glutamax supplemented with 100× MNEAA solution, 1,000× 2-mercaptoethanol, 100× StemSpan CC100, 1,000× TPO, and 10,000× GM-CSF | Full Paper |
2016 | Wang, J;DeClercq, JJ;Hayward, SB;Li, PW;Shivak, DA;Gregory, PD;Lee, G;Holmes, MC; | Highly efficient homology-driven genome editing in human T cells by combining zinc-finger nuclease mRNA and AAV6 donor delivery | Nucleic acids research | 26527725 | Fresh purified CD8 + and CD4 + T cells were purchased from AllCells.com and maintained in X-VIVO 15 (Lonza, Basel, Switzerland) supplemented with 10% FBS, 2 mM l-glutamine, 1 Save | Full Paper |
2016 | Beard, RS;Yang, X;Meegan, JE;Overstreet, JW;Yang, CG;Elliott, JA;Reynolds, JJ;Cha, BJ;Pivetti, CD;Mitchell, DA;Wu, MH;Deschenes, RJ;Yuan, SY; | Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndrome | Nature communications | 27653213 | Full Paper | |
2016 | Zhao, Y;Wang, A;Zou, Y;Su, N;Loscalzo, J;Yang, Y; | In vivo monitoring of cellular energy metabolism using SoNar, a highly responsive sensor for NAD(+)/NADH redox state | Nature protocols | 27362337 | Cells of interest: we have used the following: NCI-H1299 non-small-cell lung cancer (ATCC, cat. no. CRL-5803), primary mouse hepatocytes (freshly isolated), primary mouse renal tubular epithelial cells (AllCells, cat. no. MRTEC-001F), primary mouse a | Full Paper |
2016 | Li, L;Qi, X;Sun, W;Abdel-Azim, H;Lou, S;Zhu, H;Prasadarao, NV;Zhou, A;Shimada, H;Shudo, K;Kim, YM;Khazal, S;He, Q;Warburton, D;Wu, L; | Am80-GCSF synergizes myeloid expansion and differentiation to generate functional neutrophils that reduce neutropenia-associated infection and mortality | EMBO molecular medicine | 27737899 | opoietin. Primary cells were split every 3 days with equal portions of fresh MM‐HSCCM. Normal primary human hematopoietic precursors and cell culture Human umbilical cord blood CD34+ precursors (AllCells, Emeryville, CA) were expanded, as described | Full Paper |
2016 | Sun, H;Lin, DC;Cao, Q;Guo, X;Marijon, H;Zhao, Z;Gery, S;Xu, L;Yang, H;Pang, B;Lee, VK;Lim, HJ;Doan, N;Said, JW;Chu, P;Mayakonda, A;Thomas, T;Forscher, C;Baloglu, E;Shacham, S;Rajalingam, R;Koeffler, HP; | CRM1 Inhibition Promotes Cytotoxicity in Ewing Sarcoma Cells by Repressing EWS-FLI1-Dependent IGF-1 Signaling | Cancer research | 26956669 | IGFBP3 siRNA lentiviral plasmid (i010368) and EWS-FLI siRNAs (ABM Inc.); Matrigel anti-rabbit IgG and anti-mouse conjugated HRP antibodies (BD Biosciences). siRNA pools targeting DDIT3 and p53 were purchased from Dharmacon. Bone marrow cells from nor | Full Paper |
2016 | Prabhala, RH;Fulciniti, M;Pelluru, D;Rashid, N;Nigroiu, A;Nanjappa, P;Pai, C;Lee, S;Prabhala, NS;Bandi, RL;Smith, R;Lazo-Kallanian, SB;Valet, S;Raje, N;Gold, JS;Richardson, PG;Daley, JF;Anderson, KC;Ettenberg, SA;Di Padova, F;Munshi, NC; | Targeting IL-17A in multiple myeloma: a potential novel therapeutic approach in myeloma | Leukemia | 26293646 | med consent in accordance with the Declaration of Helsinki and approved by the institutional review board (IRB) from Dana-Farber Cancer Institute. Healthy donor bone marrow samples were obtained from AllCells (Emeryville, CA). Myeloma cell-prolifera | Full Paper |
2016 | Su, LF;Del Alcazar, D;Stelekati, E;Wherry, EJ;Davis, MM; | Antigen exposure shapes the ratio between antigen-specific Tregs and conventional T cells in human peripheral blood | Proceedings of the National Academy of Sciences of the United States of America | 27681619 | Human HLA-DR4 umbilical cord blood was purchased from AllCells and from the New York Human HLA-DR4 umbilical cord bloods were purchased from AllCells and from the New Save | Full Paper |
2016 | Ye, L;Wang, J;Tan, Y;Beyer, AI;Xie, F;Muench, MO;Kan, YW; | Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and β-thalassemia | Proceedings of the National Academy of Sciences of the United States of America | 27601644 | according to the manufacturer’s instruction. Peripheral blood mobilized CD34+ HSPCs and bone marrow CD34+ HSPCs were purchased from AllCells and cultured in StemSpan SFEM (Stemcell Technologies) supplemented with human cytokines, 100 ng/mL SCF, 100 n | Full Paper |
2016 | Tarafder, S;Koch, A;Jun, Y;Chou, C;Awadallah, MR;Lee, CH; | Micro-precise spatiotemporal delivery system embedded in 3D printing for complex tissue regeneration | Biofabrication | 27108484 | MSCs were isolated from human bone marrow (AllCells, Alameda, CA) following our established protocol [5, 13]. P3-5 MSCs at a density of 1 × 10 6 cells ml −1 were seeded into the Save | Full Paper |
2016 | Horiguchi, H;Kobune, M;Kikuchi, S;Yoshida, M;Murata, M;Murase, K;Iyama, S;Takada, K;Sato, T;Ono, K;Hashimoto, A;Tatekoshi, A;Kamihara, Y;Kawano, Y;Miyanishi, K;Sawada, N;Kato, J; | Extracellular vesicle miR-7977 is involved in hematopoietic dysfunction of mesenchymal stromal cells via poly(rC) binding protein 1 reduction in myeloid neoplasms | Haematologica | 26802051 | MSCs from healthy volunteers (HVs) were purchased from AllCells, LLC (Toronto, Canada) (HV-derived MSCs #1, #2, #3 and #4). Human primary MSCs and human BM CD34+ cells derived from lymphoma patients stage I/II without bone marrow localization (contro | Full Paper |
2016 | Li, D;Xie, K;Zhang, L;Yao, X;Li, H;Xu, Q;Wang, X;Jiang, J;Fang, J; | Dual blockade of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) exhibits potent anti-angiogenic effects | Cancer letters | 27130666 | Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China) and maintained in a complete HUVEC medium with 10% FBS (Invitrogen) and 10% Save | Full Paper |
2016 | de Jong, RN;Beurskens, FJ;Verploegen, S;Strumane, K;van Kampen, MD;Voorhorst, M;Horstman, W;Engelberts, PJ;Oostindie, SC;Wang, G;Heck, AJ;Schuurman, J;Parren, PW; | A Novel Platform for the Potentiation of Therapeutic Antibodies Based on Antigen-Dependent Formation of IgG Hexamers at the Cell Surface | PLoS biology | 26736041 | nuously cultured systems to ensure authenticity of the cell lines. Pooled normal human serum (NHS) AB was obtained from Sanquin (The Netherlands). Primary B cells from CLL patients were obtained from AllCells (Alameda, CA). Animals Eight- to 11-wk-o | Full Paper |
2016 | Carmi, Y;Prestwood, TR;Spitzer, MH;Linde, IL;Chabon, J;Reticker-Flynn, NE;Bhattacharya, N;Zhang, H;Zhang, X;Basto, PA;Burt, BM;Alonso, MN;Engleman, EG; | Akt and SHP-1 are DC-intrinsic checkpoints for tumor immunity | JCI insight | 27812544 | For some experiments, BM cells and peripheral blood from the same donor were purchased from AllCells. The cells were applied to a Ficoll gradient (BD), and CD34 + cells were Save | Full Paper |
2016 | Legemate, K;Tarafder, S;Jun, Y;Lee, CH; | Engineering Human TMJ Discs with Protein-Releasing 3D-Printed Scaffolds | Journal of dental research | 27053116 | Human MSCs were isolated from commercially available, fresh whole bone marrow samples of anonymous adult donors (AllCells) (age range: 20-25 y) per our previous methods (Lee et al. 2009; Lee, Shah, et al. 2010; Lee et al. 2014). Following our establi | Full Paper |
2016 | Kota, DJ;Prabhakara, KS;van Brummen, AJ;Bedi, S;Xue, H;DiCarlo, B;Cox, CS;Olson, SD; | Propranolol and Mesenchymal Stromal Cells Combine to Treat Traumatic Brain Injury | Stem cells translational medicine | 26586775 | We isolated MSCs from commercially available fresh human bone marrow aspirates of a 34-year-old male (AllCells, Emeryville, CA, http://www.allcells.com) according to the protocols previously established using density centrifugation and plastic adhere | Full Paper |
2016 | Lee, SJ;Levitsky, K;Parlati, F;Bennett, MK;Arastu-Kapur, S;Kellerman, L;Woo, TF;Wong, AF;Papadopoulos, KP;Niesvizky, R;Badros, AZ;Vij, R;Jagannath, S;Siegel, D;Wang, M;Ahmann, GJ;Kirk, CJ; | Clinical activity of carfilzomib correlates with inhibition of multiple proteasome subunits: application of a novel pharmacodynamic assay | British journal of haematology | 27071340 | Human whole blood and peripheral blood mononuclear cell (PBMC)-enriched leukapheresis-derived samples from healthy volunteers were purchased from AllCells (Berkeley, CA, Save | Full Paper |
2016 | Jones, RB;Mueller, S;O'Connor, R;Rimpel, K;Sloan, DD;Karel, D;Wong, HC;Jeng, EK;Thomas, AS;Whitney, JB;Lim, SY;Kovacs, C;Benko, E;Karandish, S;Huang, SH;Buzon, MJ;Lichterfeld, M;Irrinki, A;Murry, JP;Tsai, A;Yu, H;Geleziunas, R;Trocha, A;Ostrowski, MA;Irvine, DJ;Walker, BD; | A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-Lymphocytes | PLoS pathogens | 27082643 | the HIV-1 Test, v2.0 kit (Roche Diagnostics). Primary CD4+ T cell HIV latency model Total peripheral blood mononuclear cells (PBMCs) were obtained from healthy HIV-negative donors by leukapheresis (AllCells, Inc, Emeryville, CA). Naive CD4+ T cells | Full Paper |
2016 | Clark, RS;Pellom, ST;Booker, B;Ramesh, A;Zhang, T;Shanker, A;Maguire, M;Juarez, PD;Patricia, MJ;Langston, MA;Lichtveld, MY;Hood, DB; | Validation of research trajectory 1 of an Exposome framework: Exposure to benzo(a)pyrene confers enhanced susceptibility to bacterial infection | Environmental research | 26765097 | Fresh human CD14+ monocytes were obtained from AllCells (Alameda, CA) and were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated FBS (HyClone), 100 U/penicillin, 100 U/mL streptomycin (GIBCO) in the presence of DMSO, B(a)P (1, 5, 10 | Full Paper |
2016 | Zhang, W;Cao, H;Zhang, X;Li, G;Chang, Q;Zhao, J;Qiao, Y;Ding, X;Yang, G;Liu, X;Jiang, X; | A strontium-incorporated nanoporous titanium implant surface for rapid osseointegration | Nanoscale | 26881868 | In this experiment, endothelial cell growth medium (AllCells) was used to collect the secreted growth factors, and then was taken for HUVECS culture for 24 hours to form cellular Save | Full Paper |
2016 | Wu, J;Sun, Y;Block, TJ;Marinkovic, M;Zhang, ZL;Chen, R;Yin, Y;Song, J;Dean, DD;Lu, Z;Chen, XD; | Umbilical cord blood-derived non-hematopoietic stem cells retrieved and expanded on bone marrow-derived extracellular matrix display pluripotent characteristics | Stem cell research & therapy | 27906056 | Freshly isolated human BM-MNCs (containing MSCs), obtained from 20- to 30-year-old donors and purchased from ALLCELLS (Emeryville, CA, USA), were used to prepare BM stromal Save | Full Paper |
2016 | Umemura, N;Ohkoshi, E;Tajima, M;Kikuchi, H;Katayama, T;Sakagami, H; | Hyaluronan induces odontoblastic differentiation of dental pulp stem cells via CD44 | Stem cell research & therapy | 27651223 | s modified Eagle’s medium (DMEM) was purchased from Invitrogen (Carlsbad, CA, USA). Fetal bovine serum (FBS) was purchased from Nichirei Bioscience (Tokyo, Japan). Human DPSCs were obtained from AllCells LLC (Emeryville, CA, USA). Cell cultures were | Full Paper |
2016 | Frasheri, I;Ern, C;Diegritz, C;Hickel, R;Hristov, M;Folwaczny, M; | Full-length amelogenin influences the differentiation of human dental pulp stem cells | Stem cell research & therapy | 26762641 | The human dental pulp stem cells hDPSCs were obtained from AllCells (Alameda, CA, USA). They had been characterized by the company by means of flow cytometry, revealing a Save | Full Paper |
2016 | Chen, W;Wang, S;Adhikari, S;Deng, Z;Wang, L;Chen, L;Ke, M;Yang, P;Tian, R; | Simple and Integrated Spintip-Based Technology Applied for Deep Proteome Profiling | Analytical chemistry | 27062885 | The SHED cell line was purchased from Allcells (Alameda, CA) and cultured in α-modified Eagle's medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/ Save | Full Paper |
2016 | Gonçalves Silva, I;Rüegg, L;Gibbs, BF;Bardelli, M;Fruehwirth, A;Varani, L;Berger, SM;Fasler-Kan, E;Sumbayev, VV; | The immune receptor Tim-3 acts as a trafficker in a Tim-3/galectin-9 autocrine loop in human myeloid leukemia cells | Oncoimmunology | 27622049 | ). All other chemicals purchased were of the highest grade of purity. Primary human AML and CLL cells Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accorda | Full Paper |
2016 | Yang, J;Liu, W;Lv, J;Feng, Y;Ren, X;Zhang, W; | REDV-polyethyleneimine complexes for selectively enhancing gene delivery in endothelial cells | Journal of materials chemistry. B | 32263272 | The pEGFP-ZNF580 plasmid (pZNF580) was preserved by Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force. CREDVW and CRE[V with combining low line][D with combining low line]W peptides were supplie | Full Paper |
2016 | Ma, SD;Xu, X;Jones, R;Delecluse, HJ;Zumwalde, NA;Sharma, A;Gumperz, JE;Kenney, SC; | PD-1/CTLA-4 Blockade Inhibits Epstein-Barr Virus-Induced Lymphoma Growth in a Cord Blood Humanized-Mouse Model | PLoS pathogens | 27186886 | diabetic/severe combined immunodeficient (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson Labs (catalogue 005557). Commercially purchased CD34-depleted human cord blood mononuclear cells (AllCells, LLC., CB117) were mock-infected, or infect | Full Paper |
2016 | Ebina, W; | Combinatorial Pathway Modulation Toward Ex Vivo Maintenance and Propagation of Hematopoietic Stem Cells | Thesis | CD34 enriched human cord blood, mobilized peripheral blood, or human bone marrow (AllCells) were thawed, washed in PBS with 2mM EDTA and 2%FBS, and stained for the following markers: Lineage(CD235a, CD3, CD19, CD20, CD56), CD34, CD38, CD45RA, and CD9 | Full Paper | |
2016 | Yang, T;Yao, Q;Cao, F;Liu, Q;Liu, B;Wang, XH; | Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesis | International journal of nanomedicine | 27994464 | nd the human lymphoma cell lines Raji and Daudi were obtained from the American Type Culture Collection (Manassas, VA, USA). Human umbilical vein endothelial cells (HUVEC) were obtained from Shanghai AllCells Biotech Co. Ltd. (Shanghai, People’s Repu | Full Paper |
2016 | Moot, R;Raikar, SS;Fleischer, L;Querrey, M;Tylawsky, DE;Nakahara, H;Doering, CB;Spencer, HT; | Genetic engineering of chimeric antigen receptors using lamprey derived variable lymphocyte receptors | Molecular therapy oncolytics | 27933313 | d using a BD FACS Aria II Cell Sorter (BD Biosciences). Expansion and lentiviral transduction of primary human T cells Commercially available human peripheral blood mononuclear cells were purchased (AllCells, Catalog # PB004F, Alameda, CA). After th | Full Paper |
2016 | Hall, BM;Balan, V;Gleiberman, AS;Strom, E;Krasnov, P;Virtuoso, LP;Rydkina, E;Vujcic, S;Balan, K;Gitlin, I;Leonova, K;Polinsky, A;Chernova, OB;Gudkov, AV; | Aging of mice is associated with p16(Ink4a)- and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cells | Aging | 27391570 | f p16(Ink4a)-positive cells [21,23] and may reflect an exaggerated view on SCs as the sole source of inflammaging. MATERIALS AND METHODS Cell culture Primary human neonatal dermal fibroblasts (NDFs; AllCells, LLC) were pooled equally from three sepa | Full Paper |
2016 | Naka, K;Ishihara, K;Jomen, Y;Jin, CH;Kim, DH;Gu, YK;Jeong, ES;Li, S;Krause, DS;Kim, DW;Bae, E;Takihara, Y;Hirao, A;Oshima, H;Oshima, M;Ooshima, A;Sheen, YY;Kim, SJ;Kim, DK; | Novel oral transforming growth factor-β signaling inhibitor EW-7197 eradicates CML-initiating cells | Cancer science | 26583567 | Full Paper | |
2016 | Rovira Gonzalez, YI;Lynch, PJ;Thompson, EE;Stultz, BG;Hursh, DA; | In vitro cytokine licensing induces persistent permissive chromatin at the Indoleamine 2,3-dioxygenase promoter | Cytotherapy | 27421739 | Bone marrow-derived MSCs were obtained from AllCells (Alameda, CA), Lonza (Allendale, NJ), and RoosterBio (Frederick, MD) at passage one (Supplemental Table 1). MSCs in this study were previously expanded, characterized, and cryopreserved to generate | Full Paper |
2016 | Sutton, KS;Dasgupta, A;McCarty, D;Doering, CB;Spencer, HT; | Bioengineering and serum free expansion of blood-derived γδ T cells | Cytotherapy | 27260209 | Commercially available PBMCs were obtained via Leuko Pak apheresis from 11 healthy donors (AllCells). Nine frozen and two fresh PBMCs were used. Frozen PBMCs were rapidly Save | Full Paper |
2016 | Stultz, BG;McGinnis, K;Thompson, EE;Lo Surdo, JL;Bauer, SR;Hursh, DA; | Chromosomal stability of mesenchymal stromal cells during in vitro culture | Cytotherapy | 26780865 | Both AllCells and Lonza use standard protocols for isolation of MSCs from bone marrow and culture cells in Mesencult (Stem Cell Technologies) or MSCGM (Lonza), respectively. Save | Full Paper |
2016 | Imamichi, Y;Yuhki, KI;Orisaka, M;Kitano, T;Mukai, K;Ushikubi, F;Taniguchi, T;Umezawa, A;Miyamoto, K;Yazawa, T; | 11-Ketotestosterone Is a Major Androgen Produced in Human Gonads | The Journal of clinical endocrinology and metabolism | 27428878 | Plasma was separated by centrifugation at 1000g for 5 minutes. Other plasma samples were purchased from AllCells and ProMedDX. All plasma samples were collected under IRB-approved collection protocols and subject informed consent. The donors were 10 | Full Paper |
2016 | Tsiang, M;Jones, GS;Goldsmith, J;Mulato, A;Hansen, D;Kan, E;Tsai, L;Bam, RA;Stepan, G;Stray, KM;Niedziela-Majka, A;Yant, SR;Yu, H;Kukolj, G;Cihlar, T;Lazerwith, SE;White, KL;Jin, H; | Antiviral Activity of Bictegravir (GS-9883), a Novel Potent HIV-1 Integrase Strand Transfer Inhibitor with an Improved Resistance Profile | Antimicrobial agents and chemotherapy | 27645238 | the pseudotyped particles from SODk1 2G cells has been previously described (29). Freshly isolated human peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers (supplied by AllCells, Inc., Alameda, CA). Blood donors were ne | Full Paper |
2016 | Feng, JY;Xu, Y;Barauskas, O;Perry, JK;Ahmadyar, S;Stepan, G;Yu, H;Babusis, D;Park, Y;McCutcheon, K;Perron, M;Schultz, BE;Sakowicz, R;Ray, AS; | Role of Mitochondrial RNA Polymerase in the Toxicity of Nucleotide Inhibitors of Hepatitis C Virus | Antimicrobial agents and chemotherapy | 26596942 | Normal human primary bone marrow (BM) light-density cells were from three different lots obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD). Primary rat neonatal Save | Full Paper |
2016 | Yoon, J;Wang, H;Kim, YC;Yoshimoto, M;Abbasi, S;Morse Iii, HC; | Plasma cell alloantigen ENPP1 is expressed by a subset of human B cells with potential regulatory functions | Immunology and cell biology | 27029896 | 1 mm sodium pyruvate and HEPES) immediately before each experiment. Cord blood cells were purchased from AllCells LLC (Emeryville, CA. USA). Tonsillar tissues were kindly provided by Dr Leonid B Margolis (National Institute of Child Health and Human | Full Paper |
2016 | Marklein, RA;Lo Surdo, JL;Bellayr, IH;Godil, SA;Puri, RK;Bauer, SR; | High Content Imaging of Early Morphological Signatures Predicts Long Term Mineralization Capacity of Human Mesenchymal Stem Cells upon Osteogenic Induction | Stem cells (Dayton, Ohio) | 26865267 | Human bone marrow-derived stem cells were obtained from eight different donors purchased from either Lonza (Walkersville, MD, www.lonza.com) (167696, 110877, 8F3560, 127756) or All Cells (Emeryville, CA, www.allcells.com) (PCBM1632, PCBM1641, PCBM165 | Full Paper |
2016 | Rosa, V;Xie, H;Dubey, N;Madanagopal, TT;Rajan, SS;Morin, JL;Islam, I;Castro Neto, AH; | Graphene oxide-based substrate: physical and surface characterization, cytocompatibility and differentiation potential of dental pulp stem cells | Dental materials : official publication of the Academy of Dental Materials | 27283997 | The use of human cells in this study was approved by NUS Institutional Review Board (Approval Number: NUS 2094). Human dental pulp stem cells from single donor (DPF003, Allcells, USA) were sorted by fluorescence-activated cell sorting analysis (FACS, | Full Paper |
2016 | Wang, J;Zhang, Y;Zhu, Q;Liu, Y;Cheng, H;Zhang, Y;Li, T; | Emodin protects mice against radiation-induced mortality and intestinal injury via inhibition of apoptosis and modulation of p53 | Environmental toxicology and pharmacology | 27525562 | The aim of this study was to explore the protective effect of emodin, a plant-derived anthraquinone, against gamma radiation-induced mortality and intestinal injury in mice, and to Save | Full Paper |
2016 | Walters, MC;De Castro, LM;Sullivan, KM;Krishnamurti, L;Kamani, N;Bredeson, C;Neuberg, D;Hassell, KL;Farnia, S;Campbell, A;Petersdorf, E; | Indications and Results of HLA-Identical Sibling Hematopoietic Cell Transplantation for Sickle Cell Disease | Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation | 26500093 | Financial disclosure: MCW is medical director for the ViaCord Processing Laboratory and AllCells, Inc. He is a consultant for bluebird bio, Inc, Sangamo Biosciences, Inc and Bayer. Save | Full Paper |
2016 | Hsu, H;Boudova, S;Mvula, G;Divala, TH;Mungwira, RG;Harman, C;Laufer, MK;Pauza, CD;Cairo, C; | Prolonged PD1 Expression on Neonatal Vδ2 Lymphocytes Dampens Proinflammatory Responses: Role of Epigenetic Regulation | Journal of immunology (Baltimore, Md. : 1950) | 27474072 | commercial specimens obtained from AllCells. Although we cannot exclude that we introduced a bias in our study, this seems unlikely considering that the AllCells specimens were not Save | Full Paper |
2016 | Chen, LT;Hu, MM;Xu, ZS;Liu, Y;Shu, HB; | MSX1 Modulates RLR-Mediated Innate Antiviral Signaling by Facilitating Assembly of TBK1-Associated Complexes | Journal of immunology (Baltimore, Md. : 1950) | 27194789 | Generation and transfection of human primary macrophages were performed, as previously described (36). The human CD14+ monocytes were purchased from ALLCELLS. For generation of macrophages, the CD14+ monocytes were treated with recombinant M-CSF (50 | Full Paper |
2016 | Gao, W;Liu, H;Yuan, J;Wu, C;Huang, D;Ma, Y;Zhu, J;Ma, L;Guo, J;Shi, H;Zou, Y;Ge, J; | Exosomes derived from mature dendritic cells increase endothelial inflammation and atherosclerosis via membrane TNF-α mediated NF-κB pathway | Journal of cellular and molecular medicine | 27515767 | P (Ribobio, Guangzhou, China) was used. SiRNA TNF‐α was also purchased from Ribobio and the transfected concentration was 50 nM. HUVECs culture and intervention Primary HUVECs were purchased from AllCells (Shanghai, China) and cultured with a specia | Full Paper |
2016 | Zhou, Y;Li, XH;Zhang, CC;Wang, MJ;Xue, WL;Wu, DD;Ma, FF;Li, WW;Tao, BB;Zhu, YC; | Hydrogen sulfide promotes angiogenesis by downregulating miR-640 via the VEGFR2/mTOR pathway | American journal of physiology. Cell physiology | 26879375 | Pooled human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in endothelial basal medium (EBM; Allcells) supplemented with Save | Full Paper |
2016 | Lowery, JW;Ankrum, JA;Kokabu, S;Liu, R; | Targeted Strategies to Modulate Stem-Cell-Relevant Pathways | Stem cells international | 27703484 | + CB progenitors (also referred to as "starting CB progenitors") from pooled donors were purchased from AllCells (Emeryville, CA) and cultured in the hematopoietic growth medium ( Save | Full Paper |
2016 | Nagaria, PK;Robert, C;Park, TS;Huo, JS;Zambidis, ET;Rassool, FV; | High-Fidelity Reprogrammed Human IPSCs Have a High Efficacy of DNA Repair and Resemble hESCs in Their MYC Transcriptional Signature | Stem cells international | 27688775 | + CB progenitors (also referred to as "starting CB progenitors") from pooled donors were purchased from AllCells (Emeryville, CA) and cultured in the hematopoietic growth medium ( Save | Full Paper |
2016 | Shibata, T;Ohto, U;Nomura, S;Kibata, K;Motoi, Y;Zhang, Y;Murakami, Y;Fukui, R;Ishimoto, T;Sano, S;Ito, T;Shimizu, T;Miyake, K; | Guanosine and its modified derivatives are endogenous ligands for TLR7 | International immunology | 26489884 | Human macrophages were induced from human peripheral blood mononuclear cells (hPBMCs), purchased from Allcells (Alameda, CA, USA) and Cellular Technology Limited (Shaker Height, OH, USA). hPBMCs were plated in 10-cm cell-culture dishes with a density | Full Paper |
2016 | Pineda, G;Lennon, KM;Delos Santos, NP;Lambert-Fliszar, F;Riso, GL;Lazzari, E;Marra, MA;Morris, S;Sakaue-Sawano, A;Miyawaki, A;Jamieson, CH; | Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined Niche | Scientific reports | 27041210 | amples were obtained from patient peripheral blood that was collected with informed consent for research use in accordance with Institutional Review Board approved protocols at UCSD or purchased from AllCells (Alameda, CA). Normal peripheral blood mo | Full Paper |
2016 | Zou, L;Chen, Q;Quanbeck, Z;Bechtold, JE;Kaufman, DS; | Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells | Scientific reports | 26980556 | Human BM-MSCs were isolated from fresh bone marrow (AllCells) according to the recommended protocol and expanded in MSC culture media, consisting of 10% defined fetal bovine Save | Full Paper |
2016 | Ling, C;Bhukhai, K;Yin, Z;Tan, M;Yoder, MC;Leboulch, P;Payen, E;Srivastava, A; | High-Efficiency Transduction of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for Overcoming Donor-Variation and Implications in Genome Editing | Scientific reports | 27759036 | ary HSPCs derived from bone marrow (BM) as well as from umbilical cord blood (CB). BM-derived CD34+ cells from individual donors (or a mixture from 10 donors) were purchased from a commercial source (AllCells, LLC, Alameda, CA, USA), and were used to | Full Paper |
2016 | Tian, GA;Zhu, CC;Zhang, XX;Zhu, L;Yang, XM;Jiang, SH;Li, RK;Tu, L;Wang, Y;Zhuang, C;He, P;Li, Q;Cao, XY;Cao, H;Zhang, ZG; | CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinib | Scientific reports | 27506146 | HUVECs were obtained from the Fifth People's Hospital of Shanghai and cultured in endothelial cell complete medium containing endothelial cell growth supplement (Allcells; H-004). Save | Full Paper |
2016 | Kim, YC;Cui, J;Luo, J;Xiao, F;Downs, B;Wang, SM; | Exome-based Variant Detection in Core Promoters | Scientific reports | 27464681 | Caucasian male donor were purchased from AllCells Company (AllCells, Alameda, California). The use of the cells for research purpose has AllCells company IRB approval (Biomed Save | Full Paper |
2016 | Chalmers, SA;Doerner, J;Bosanac, T;Khalil, S;Smith, D;Harcken, C;Dimock, J;Der, E;Herlitz, L;Webb, D;Seccareccia, E;Feng, D;Fine, JS;Ramanujam, M;Klein, E;Putterman, C; | Therapeutic Blockade of Immune Complex-Mediated Glomerulonephritis by Highly Selective Inhibition of Bruton's Tyrosine Kinase | Scientific reports | 27192942 | Primary CD19+ cells were purified from human peripheral blood mononuclear cells (AllCells frozen peripheral blood mononuclear cells (AllCells) and negatively selected by magnetic Save | Full Paper |
2016 | Moisseiev, E;Smit-McBride, Z;Oltjen, S;Zhang, P;Zawadzki, RJ;Motta, M;Murphy, CJ;Cary, W;Annett, G;Nolta, JA;Park, SS; | Intravitreal Administration of Human Bone Marrow CD34+ Stem Cells in a Murine Model of Retinal Degeneration | Investigative ophthalmology & visual science | 27537262 | Fresh human BM aspirate was purchased from AllCells (Alameda, CA, USA) and obtained for the scheduled day of use. The BM aspirate for this study was obtained from a 38-year-old Save | Full Paper |
2016 | Hassan, HE;Keita, JA;Narayan, L;Brady, SM;Frederick, R;Carlson, S;C Glass, K;Natesan, S;Buttolph, T;Fandy, TE; | The combination of dimethoxycurcumin with DNA methylation inhibitor enhances gene re-expression of promoter-methylated genes and antagonizes their cytotoxic effect | Epigenetics | 27588609 | Frozen acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) bone marrow mononuclear cells (BMNC) were purchased from Allcells (Alameda, CA). BMNC were obtained from newly diagnosed and untreated patients. The viability of the cells was tes | Full Paper |
2016 | Hu, X;Hu, T;Shen, G;Lian, M;Guan, G;Wang, F;Wang, L; | PCL films of varying porosity influence ICAM-1 expression of HUVECs | Journal of biomedical materials research. Part A | 27345288 | The HUVECs (HUVEC001) provided by Allcells Company (Shanghai, China) were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco), and 1% penicillin-streptomycin (Gibco) at 37°C in a 5% CO2 incubator. Before see | Full Paper |
2016 | Lin, HA;Gupta, MS;Varma, DM;Gilchrist, ML;Nicoll, SB; | Lower crosslinking density enhances functional nucleus pulposus-like matrix elaboration by human mesenchymal stem cells in carboxymethylcellulose hydrogels | Journal of biomedical materials research. Part A | 26256108 | Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density gradient centrifugation as previously described.18 Briefly, bone marrow aspirate was Save | Full Paper |
2016 | Rogers, LJ;Basnakian, AG;Orloff, MS;Ning, B;Yao-Borengasser, A;Raj, V;Kadlubar, S; | 2-amino-1-methyl-6-phenylimidazo(4,5-b) pyridine (PhIP) induces gene expression changes in JAK/STAT and MAPK pathways related to inflammation, diabetes and cancer | Nutrition & metabolism | 27547236 | med by submission to American Type Culture Collection (ATCC) (Manassas, VA) for analysis by short tandem repeat methodology on December 9, 2015. Human Mesenchymal Stem Cells (hMSC) were obtained from AllCells (Emeryville, CA). The hMSCs were expanded | Full Paper |
2016 | Pellegrino, M;Sciambi, A;Yates, JL;Mast, JD;Silver, C;Eastburn, DJ; | RNA-Seq following PCR-based sorting reveals rare cell transcriptional signatures | BMC genomics | 27189161 | Anonymous blood samples were initially obtained from a commercial provider (AllCells) that operates with independent IRB approval. Donor anonymity was protected using United Save | Full Paper |
2016 | Holt, A;Wootton, SC;Mulero, JJ;Brzoska, PM;Langit, E;Green, RL; | Developmental validation of the Quantifiler(®) HP and Trio Kits for human DNA quantification in forensic samples | Forensic science international. Genetics | 26774100 | Human female DNA "A3408" was purified from peripheral blood cells obtained from AllCells LLC. (Alameda, CA) using a QIAamp DNA Blood Maxi Kit (Qiagen). Save | Full Paper |
2016 | Pankajakshan, D;Albuquerque, MT;Evans, JD;Kamocka, MM;Gregory, RL;Bottino, MC; | Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function | Journal of endodontics | 27663615 | After 7 days, the specimens were rinsed with 10 mL saline followed by 3 mL EDTA for 3 minutes, and a final abundant rinsing step with saline was performed before cell seeding. Human DPSCs (AllCells, LLC, Alameda, CA) were cultured in alpha-minimum es | Full Paper |
2016 | Nicoletti, AM;Kenny, CH;Khalil, AM;Pan, Q;Ralph, KL;Ritchie, J;Venkataramani, S;Presky, DH;DeWire, SM;Brodeur, SR; | Unexpected Potency Differences between B-Cell-Activating Factor (BAFF) Antagonist Antibodies against Various Forms of BAFF: Trimer, 60-Mer, and Membrane-Bound | The Journal of pharmacology and experimental therapeutics | 27440419 | Negatively selected B cells were obtained from frozen human peripheral blood (AllCells, LLC, Alameda, CA). Sexes of cell donors were as follows: Fig. 2C = 60-mer, two males, one Save | Full Paper |
2016 | Jin, E;Kim, TH;Han, S;Kim, SW; | Amniotic epithelial cells promote wound healing in mice through high epithelialization and engraftment | Journal of tissue engineering and regenerative medicine | 26174407 | Human AMEs were from AllCells (Alameda, CA, USA) and adipose-derived mesenchymal stem cells (ADMs) were from Thermo Scientific Inc. (Rockford, IL, USA). Human keratinocytes were also purchased from ATCC. Cell culture was performed as described previo | Full Paper |
2016 | Yang, H;Sun, J;Li, Y;Duan, WM;Bi, J;Qu, T; | Human umbilical cord-derived mesenchymal stem cells suppress proliferation of PHA-activated lymphocytes in vitro by inducing CD4(+)CD25(high)CD45RA(+) regulatory T cell production and modulating cytokine secretion | Cellular immunology | 26774852 | PBMCs of healthy donors (n = 10) were purchased from AllCells Inc (Emeryville, CA, USA) and StemExpress (Placerville, CA, USA). Co-culture experiments were performed in a 12-well plate (BD, Franklin Lakes, New Jersey, USA). 1 × 105 UC-MSCs were plate | Full Paper |
2016 | Foster, MH;Buckley, ES;Chen, BJ;Hwang, KK;Clark, AG; | Uncommon structural motifs dominate the antigen binding site in human autoantibodies reactive with basement membrane collagen | Molecular immunology | 27450516 | 100 cGy, X-RAD 320, Precision X-ray, North Branford, CT) 9.5-10 week old NSG recipients were intravenously injected with purified human CD34+ HSC (AllCells LLC, Alameda, CA), using 2.5-10×104 CD34+ HSC per mouse (Pearson et al., 2008; Shultz et al., | Full Paper |
2016 | Smith, AJ;Li, Y;Bazin, HG;St-Jean, JR;Larocque, D;Evans, JT;Baldridge, JR; | Evaluation of novel synthetic TLR7/8 agonists as vaccine adjuvants | Vaccine | 27402566 | Human myeloid or plasmacytoid DCs were isolated from PBMCs obtained by leukapheresis from normal donors (AllCells, Berkeley, CA). PBMCs were repeatedly washed in 1× PBS Save | Full Paper |
2016 | Zeng, Y;Liu, J; | Role of glypican-1 in endothelial NOS activation under various steady shear stress magnitudes | Experimental cell research | 27688027 | Human umbilical vein endothelial cells (HUVECs) and all cell culture reagents were purchased from Allcells, China. As previously described [23], HUVECs were cultured in HUVEC medium containing 10% FBS and 10% growth factor supplement containing EGF, | Full Paper |
2016 | Liu, X;Zhang, A;Xiang, J;Lv, Y;Zhang, X; | miR-451 acts as a suppressor of angiogenesis in hepatocellular carcinoma by targeting the IL-6R-STAT3 pathway | Oncology reports | 27461244 | The umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Shanghai, China). HepG2 and HEK293T cells were incubated with Dulbecco's modified Eagle's medium ( Save | Full Paper |
2016 | Peterson, SJ;Vanella, L;Gotlinger, K;Jiang, H;Singh, SP;Sodhi, K;Maher, E;O'Hanlon, K;Shapiro, JI;Abraham, NG; | Oxidized HDL is a potent inducer of adipogenesis and causes activation of the Ang-II and 20-HETE systems in human obese females | Prostaglandins & other lipid mediators | 27179555 | Frozen human bone marrow mononuclear stem cells (MSCs) were purchased from Allcells (Allcells, Emeryville, CA). After thawing, mononuclear cells were resuspended in a minimal essential medium, (Invitrogen, Carlsbad, CA) supplemented with 10% heat ina | Full Paper |
2016 | Ren, X;Li, J;Xu, X;Wang, C;Cheng, Y; | IBI302, a promising candidate for AMD treatment, targeting both the VEGF and complement system with high binding affinity in vitro and effective targeting of the ocular tissue in healthy rhesus monkeys | Experimental eye research | 26919788 | HUVEC basal medium (used for HUVEC proliferation assay) was bought from Allcells (Shanghai, China). Rabbit red blood cells (RRBCs) were purchased from Laboratory animal Save | Full Paper |
2016 | Griffin, DO;Goff, SP; | Restriction of HIV-1-based lentiviral vectors in adult primary marrow-derived and peripheral mobilized human CD34+ hematopoietic stem and progenitor cells occurs prior to viral DNA integration | Retrovirology | 26945863 | ep toward uncovering the molecular mechanism restricting HIV-1 transduction of HPSCs. Methods Source of human cells We obtained primary adult marrow-derived and peripheral mobilized CD34+ cells from AllCells and StemCell Technologies (pre-isolated). | Full Paper |
2016 | Cao, L;Weetall, M;Bombard, J;Qi, H;Arasu, T;Lennox, W;Hedrick, J;Sheedy, J;Risher, N;Brooks, PC;Trifillis, P;Trotta, C;Moon, YC;Babiak, J;Almstead, NG;Colacino, JM;Davis, TW;Peltz, SW; | Discovery of Novel Small Molecule Inhibitors of VEGF Expression in Tumor Cells Using a Cell-Based High Throughput Screening Platform | PloS one | 27992500 | All primary cells were purchased from AllCells, Inc (California, USA) and cultured in RPMI-1640 medium for peripheral blood mononuclear cells (PBMC) or DMEM for adherent cells, Save | Full Paper |
2016 | Pogue, SL;Taura, T;Bi, M;Yun, Y;Sho, A;Mikesell, G;Behrens, C;Sokolovsky, M;Hallak, H;Rosenstock, M;Sanchez, E;Chen, H;Berenson, J;Doyle, A;Nock, S;Wilson, DS; | Targeting Attenuated Interferon-α to Myeloma Cells with a CD38 Antibody Induces Potent Tumor Regression with Reduced Off-Target Activity | PloS one | 27611189 | Bone Marrow cells: Normal and myeloma bone marrow cells were purchased from AllCells, Inc. as frozen vials containing ten million bone marrow mononuclear cells/vial. All donors Save | Full Paper |
2016 | Doss, JF;Jonassaint, JC;Garrett, ME;Ashley-Koch, AE;Telen, MJ;Chi, JT; | Phase 1 Study of a Sulforaphane-Containing Broccoli Sprout Homogenate for Sickle Cell Disease | PloS one | 27071063 | preferably the same time of day, once daily for 21 days. Patients consumed one (50g), two (100g) or three (150g) packages, depending on the dose. Cell culture Mobilized peripheral blood CD34+ cells (AllCells) were grown in StemLine II media containi | Full Paper |
2016 | Maifeld, SV;Ro, B;Mok, H;Chu, M;Yu, L;Yamagata, R;Leonardson, T;Chio, V;Parhy, B;Park, S;Carlson, M;Machhi, S;Ulbrandt, N;Falsey, AR;Walsh, EE;Wang, CK;Esser, MT;Zuo, F; | Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial Virus | PloS one | 27070145 | serum samples showing a ≥4-fold rise in at least one ELISA assay. Thirty pediatric serum samples were purchased from Bioreclamation (Hicksville, NY). An adult human plasma sample was purchased from AllCells (Emeryville, CA) and subsequently used as t | Full Paper |
2016 | Smith, SH;Peredo, CE;Takeda, Y;Bui, T;Neil, J;Rickard, D;Millerman, E;Therrien, JP;Nicodeme, E;Brusq, JM;Birault, V;Viviani, F;Hofland, H;Jetten, AM;Cote-Sierra, J; | Development of a Topical Treatment for Psoriasis Targeting RORγ: From Bench to Skin | PloS one | 26870941 | ions were carried out in triplicate. Sequences of primers for ChIP-qPCR are listed in S1 Appendix. Human peripheral blood CD4+ T cell cultures and cytokine analysis Cryopreserved human CD4+ T cells (AllCells, LLC and Stemcell Technologies, Inc.) wer | Full Paper |
2016 | Toby, GG;Liu, T;Buyue, Y;Zhang, X;Bitonti, AJ;Pierce, GF;Sommer, JM;Jiang, H;Peters, RT; | Recombinant Factor IX Fc Fusion Protein Maintains Full Procoagulant Properties and Exhibits Prolonged Efficacy in Hemophilia B Mice | PloS one | 26840952 | e preparation (L-α-phosphatidylserine [PS] and L-α-phosphatidylcholine [PC]) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Fresh platelets from normal volunteers were purchased from AllCells, LLC (Emeryville, CA, USA). The mini-extrud | Full Paper |
2016 | Rebbapragada, I;Birkus, G;Perry, J;Xing, W;Kwon, H;Pflanz, S; | Molecular Determinants of GS-9620-Dependent TLR7 Activation | PloS one | 26784926 | Human PBMCs were isolated from fresh whole blood derived from healthy human volunteers (AllCells) using standard Ficoll density gradient separation techniques. PBMCs were Save | Full Paper |
2016 | Matsushita, M;Otsuka, Y;Tsutsumida, N;Tanaka, C;Uchiumi, A;Ozawa, K;Suzuki, T;Ichikawa, D;Aburatani, H;Okamoto, S;Kawakami, Y;Hattori, Y; | Identification of Novel HLA-A*24:02-Restricted Epitope Derived from a Homeobox Protein Expressed in Hematological Malignancies | PloS one | 26784514 | D, Oslo, Norway) and cryopreserved in Cell Banker reagent (Juji-Field, Tokyo, Japan) in liquid nitrogen until further use. Bone marrow mononuclear cells (BMMNC) from healthy donors were obtained from AllCells, LLC. (Alameda, CA). This study was appro | Full Paper |
2016 | Manser, M; | Influence of extremely-low-frequency magnetic fields on epigenetic programming and cellular differentiation | Thesis | trichostatin A (TSA). CD34-positive cells isolated from human cord blood of mixed donors were obtained from AllCells (Alabama, United States). CD34+ cells were propagated in Stemline II medium expansion medium (Stemcell technology) supplemented with | Full Paper | |
2016 | Cabrero, M;Wei, Y;Yang, H;Ganan-Gomez, I;Bohannan, Z;Colla, S;Marchesini, M;Bravo, GM;Takahashi, K;Bueso-Ramos, C;Garcia-Manero, G; | Down-regulation of EZH2 expression in myelodysplastic syndromes | Leukemia research | 26970171 | Bone marrows from healthy individuals were obtained from AllCells (Emeryville, CA). Bone marrow mononuclear cells were isolated using a standard Ficoll gradient approach. Isolation Save | Full Paper |
2016 | Salzano, G;Zappavigna, S;Luce, A;D'Onofrio, N;Balestrieri, ML;Grimaldi, A;Lusa, S;Ingrosso, D;Artuso, S;Porru, M;Leonetti, C;Caraglia, M;De Rosa, G; | Transferrin-Targeted Nanoparticles Containing Zoledronic Acid as a Potential Tool to Inhibit Glioblastoma Growth | Journal of biomedical nanotechnology | 27301207 | Viable human bone marrow cells (purchased as frozen vials from AllCells via StemCell Technologies) were prepared with a DNase treatment (Stem Cell Technologies) and then Save | Full Paper |
2016 | Phillips, JE;Renteria, L;Burns, L;Harris, P;Peng, R;Bauer, CM;Laine, D;Stevenson, CS; | Btk Inhibitor RN983 Delivered by Dry Powder Nose-only Aerosol Inhalation Inhibits Bronchoconstriction and Pulmonary Inflammation in the Ovalbumin Allergic Mouse Model of Asthma | Journal of aerosol medicine and pulmonary drug delivery | 27111445 | Mast cells One million human cord blood derived CD34+ hematopoietic stem cells (HSCs) from different donors (AllCells #CB008F-S, Emeryville, CA) were cultured for 8 weeks in a serum-free complete medium (StemPro-34 with supplements; Invitrogen, Carls | Full Paper |
2016 | Wang, X;Zhao, S;Su, M;Sun, L;Zhang, S;Wang, D;Liu, Z;Yuan, Y;Liu, Y;Li, Y; | Geraniol improves endothelial function by inhibiting NOX-2 derived oxidative stress in high fat diet fed mice | Biochemical and biophysical research communications | 27107694 | In the present study, the in vivo diet induced obese setting was reproduced in vitro by exposing HUVECs to palmitic acid (PA), the most common circulating free fatty acid, with or without geraniol. Human umbilical vein endothelial cells (HUVECs) were | Full Paper |
2016 | Xia, T;Guan, W;Fu, J;Zou, X;Han, Y;Chen, C;Zhou, L;Zeng, C;Wang, WE; | Tirofiban induces vasorelaxation of the coronary artery via an endothelium-dependent NO-cGMP signaling by activating the PI3K/Akt/eNOS pathway | Biochemical and biophysical research communications | 27018249 | Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCELLS (Shanghai, China). HUVECs were cultured at 37 °C on gelatin-coated plates in the basal nutrient media MCDB-131 supplemented with 5% fetal bovine serum (FBS; Hyclone, Logan, | Full Paper |
2016 | Cao, Z;Wang, H;Mao, X;Luo, L; | Noncanonical function of threonyl-tRNA synthetase regulates vascular development in zebrafish | Biochemical and biophysical research communications | 26993167 | Human umbilical vein endothelial cells (HUVECs, ALLCELLS) were raised according to standard protocol. Cell immunostaining was performed as previously described [15] using Save | Full Paper |
2016 | Sugino, N;Miura, Y;Yao, H;Iwasa, M;Fujishiro, A;Fujii, S;Hirai, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T; | Early osteoinductive human bone marrow mesenchymal stromal/stem cells support an enhanced hematopoietic cell expansion with altered chemotaxis- and adhesion-related gene expression profiles | Biochemical and biophysical research communications | 26707642 | Normal human BM samples that were obtained from healthy adult volunteers with informed consent were purchased from AllCells (Emeryville, CA). Human BM-MSCs were isolated and cultured based on a previously published method [3], [12], [13], [14], [15]. | Full Paper |
2016 | Koestler, DC;Jones, MJ;Usset, J;Christensen, BC;Butler, RA;Kobor, MS;Wiencke, JK;Kelsey, KT; | Improving cell mixture deconvolution by identifying optimal DNA methylation libraries (IDOL) | BMC bioinformatics | 26956433 | Purified granulocytes, monocytes, CD4T, CD8T, natural killer cells, and B cells from normal human subjects were purchased from AllCells LLC (Emeryville, CA). As described (http://www Save | Full Paper |
2016 | Sarkar, S;Sabhachandani, P;Stroopinsky, D;Palmer, K;Cohen, N;Rosenblatt, J;Avigan, D;Konry, T; | Dynamic analysis of immune and cancer cell interactions at single cell level in microfluidic droplets | Biomicrofluidics | 27795747 | RPMI-8226 (multiple myeloma (MM) cell line) was purchased from the American Type Culture Collection (ATCC, Manassas, VA). CD8+ T cells were purchased from AllCells (#PB009-3F, AllCells, Alameda, CA). Cells were maintained in RPMI-1640 medium suppleme | Full Paper |
2016 | Kikushige, Y;Akashi, K; | A TIM-3/GAL-9 autocrine stimulatory loop drives self-renewal of human myeloid leukemia stem cells and leukemic progression | Experimental Hematology | Human adult BM and PB samples were obtained from healthy donors or purchased from AllCells. CB cells were obtained from full-term deliveries (provided by the Kyushu Block Red Save | Full Paper | |
2016 | Itokazu, M;Wakitani, S;Mera, H;Tamamura, Y;Sato, Y;Takagi, M;Nakamura, H; | Transplantation of Scaffold-Free Cartilage-Like Cell-Sheets Made from Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair: A Preclinical Study | Cartilage | 27688844 | hBMSCs were prepared from three male donors aged 21 (#1), 27 (#2), and 31 (#3) years and from one commercially available bone marrow (Allcells LLC, Alameda, CA, USA) from a 20-year-old male (#4). The donor cells were derived from bone marrow aspirate | Full Paper |
2016 | Yan, Z;Liu, J;Xie, L;Liu, X;Zeng, Y; | Role of heparan sulfate in mediating CXCL8-induced endothelial cell migration | PeerJ | 26870616 | Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells, China. HUVECs were grown in complete culture media for HUVECs (Allcells, China) in a humidified 5%/ Save | Full Paper |
2016 | Hara, C;Kubota, S;Nishida, T;Hiasa, M;Hattori, T;Aoyama, E;Moriyama, Y;Kamioka, H;Takigawa, M; | Involvement of multiple CCN family members in platelets that support regeneration of joint tissues | Modern rheumatology | 26915735 | Human platelets from a healthy donor were provided by AllCells LLC (Berkley, CA). Protein extraction from the cells was performed with RIPA buffer (50 mM Tris-HCl, 0.15 M NaCl, 4 Save | Full Paper |
2016 | Zhang, M;Jiang, L; | Oxidized low-density lipoprotein decreases VEGFR2 expression in HUVECs and impairs angiogenesis | Experimental and therapeutic medicine | 28105106 | Cell culture and LDL treatment In total 2 × 105 HUVECs (AllCells, LLC, Shanghai, China) were cultured for 3-5 passages in Dulbecco's modified Eagle's medium (DMEM; PromoCell GmbH, Heidelberg, Germany) supplemented with 10% fetal bovine serum (unless | Full Paper |
2016 | Heng, BC;Zhu, S;Xu, J;Yuan, C;Gong, T;Zhang, C; | Effects of decellularized matrices derived from periodontal ligament stem cells and SHED on the adhesion, proliferation and osteogenic differentiation of human dental pulp stem cells in vitro | Tissue & cell | 26796232 | Human DPSC (Cat No. DP003F) and SHED (Cat No. DP004F) were obtained from AllCells LLC. (Alameda, CA, USA). Human PDLSC were obtained as a gift from the School of Stomatology of Fujian Medical School (Fujian, China) and were isolated as previously des | Full Paper |
2016 | Klingelhöffer, C;Reck, A;Morsczeck, C; | The extracellular concentration of osteocalcin decreased in dental follicle cell cultures during biomineralization | Cytotechnology | 27447177 | Human dental follicle cells (DFCs) were purchased from ALLCells (Alameda, CA, USA). DFCs were cultivated in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, Munich, Germany), supplemented with 10 % fetal bovine serum, FBS (Sigma-Aldrich) and 1 | Full Paper |
2016 | Ahmed, HM;Luddin, N;Kannan, TP;Mokhtar, KI;Ahmad, A; | Chemical analysis and biological properties of two different formulations of white portland cements | Scanning | 26382064 | DPSCs cell line (AllCells, USA) obtained from biopsies of the dental pulp of adult teeth was (BCGM) (AllCells, USA), with 50 mL of human MSC stimulatory supplements (AllCells, USA). Save | Full Paper |
2016 | Mulato, A;Hansen, D;Thielen, A;Porter, D;Stepan, G;White, K;Daeumer, M;Cihlar, T;Yant, SR; | Rapid In Vitro Evaluation of Antiretroviral Barrier to Resistance at Therapeutic Drug Levels | AIDS research and human retroviruses | 27356854 | Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh leukopaks obtained from healthy volunteers under informed consent (AllCells, Inc., Alameda, CA) and Save | Full Paper |
2016 | Kowolik, CM;Lin, M;Xie, J;Overman, LE;Horne, DA; | NT1721, a novel epidithiodiketopiperazine, exhibits potent in vitro and in vivo efficacy against acute myeloid leukemia | Oncotarget | 27863389 | We then tested the effect of NT1721 on primary AML samples (from AllCells, obtained from two newly diagnosed AML patients with white blood cell counts > 10 11 /L) by treating the Save | Full Paper |
2016 | Jochems, C;Hodge, JW;Fantini, M;Fujii, R;Morillon, YM;Greiner, JW;Padget, MR;Tritsch, SR;Tsang, KY;Campbell, KS;Klingemann, H;Boissel, L;Rabizadeh, S;Soon-Shiong, P;Schlom, J; | An NK cell line (haNK) expressing high levels of granzyme and engineered to express the high affinity CD16 allele | Oncotarget | 27861156 | um iodide (AO/PI) staining on a Cellometer Auto 2000 (Nexcelom, San Mateo, CA), according to the manufacturer's instructions. Release of IL-2 into culture supernatants was measured with an ELISA kit (AllCells, Alameda, CA). Antibodies and flow cytom | Full Paper |
2016 | Gołąb, K;Grose, R;Trzonkowski, P;Wickrema, A;Tibudan, M;Marek-Trzonkowska, N;Matosz, S;Solomina, J;Ostrega, D;Michael Millis, J;Witkowski, P; | Utilization of leukapheresis and CD4 positive selection in Treg isolation and the ex-vivo expansion for a clinical application in transplantation and autoimmune disorders | Oncotarget | 27821811 | We obtained de-identified human cell leukapheresis product for the all experiments from the commercial vendor AllCells, Alameda, CA, USA, therefore the study was exempt from the Save | Full Paper |
2016 | Yang, H;Sun, J;Wang, F;Li, Y;Bi, J;Qu, T; | Umbilical cord-derived mesenchymal stem cells reversed the suppressive deficiency of T regulatory cells from peripheral blood of patients with multiple sclerosis in a co-culture - a preliminary study | Oncotarget | 27705922 | RRMS, n=12, female=8, male=4), mean age 53.75 years old (49 years to 64 years old) and healthy donors (n=10, female=4, male=6), mean age 28.38 years old (22 years to 39 years old) were purchased from AllCells Inc (Emeryville, CA) or StemExpress (Plac | Full Paper |
2016 | Xu, Y;Wang, K;Yu, Q; | FRMD6 inhibits human glioblastoma growth and progression by negatively regulating activity of receptor tyrosine kinases | Oncotarget | 27661120 | entific. Secondary anti-rabbit Alexa Fluor 594 and anti-mouse Alexa Fluor 488 were from Invitrogen. Cells, GBM cell lines, and primary GBM cells Normal human astrocytes (NHAs) were obtained from ALLCELLS, Inc and ScienCell. SF126, SF188, SF210, SF | Full Paper |
2016 | Ferluga, S;Tomé, CM;Herpai, DM;D'Agostino, R;Debinski, W; | Simultaneous targeting of Eph receptors in glioblastoma | Oncotarget | 27494882 | r Center of Wake Forest University). Stacked 2D images were obtained in some cases. Images were processed with OlyVIA V2.8. Dendritic cells Myeloid-derived naïve dendritic cells were purchased from AllCells LLC (Alameda, CA). Cells were grown in RPM | Full Paper |
2016 | Sumbayev, VV;Gonçalves Silva, I;Blackburn, J;Gibbs, BF;Yasinska, IM;Garrett, MD;Tonevitsky, AG;Ushkaryov, YA; | Expression of functional neuronal receptor latrophilin 1 in human acute myeloid leukaemia cells | Oncotarget | 27322212 | oetal calf serum, penicillin (50 IU/ml) and streptomycin sulphate (50 μg/ml). Primary human AML cells Primary human AML mononuclear cells (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordan | Full Paper |
2016 | Wang, Y;Zhan, Z;Jiang, X;Peng, X;Shen, Y;Chen, F;Ji, Y;Liu, W;Shi, Y;Duan, W;Ding, J;Ai, J;Geng, M; | Simm530, a novel and highly selective c-Met inhibitor, blocks c-Met-stimulated signaling and neoplastic activities | Oncotarget | 27191264 | rom Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine (Shanghai, China). SK-OV-3 was obtained from Fudan University (Shanghai, China). HUVEC was obtained from AllCells Bio (Shanghai, China). Cells were routine | Full Paper |
2016 | Ye, BG;Sun, HC;Zhu, XD;Chai, ZT;Zhang, YY;Ao, JY;Cai, H;Ma, DN;Wang, CH;Qin, CD;Gao, DM;Tang, ZY; | Reduced expression of CD109 in tumor-associated endothelial cells promotes tumor progression by paracrine interleukin-8 in hepatocellular carcinoma | Oncotarget | 27121053 | (Sigma-Aldrich, St. Louis, MO, USA) in a humidified atmosphere of 5% CO2 at 37°C. Human normal liver L-02 cells (Chinese Academy of Sciences) were cultured under the same conditions. Primary HUVEC (AllCells, Shanghai, China) were cultured in endothel | Full Paper |
2016 | Schwaederle, M;Husain, H;Fanta, PT;Piccioni, DE;Kesari, S;Schwab, RB;Banks, KC;Lanman, RB;Talasaz, A;Parker, BA;Kurzrock, R; | Detection rate of actionable mutations in diverse cancers using a biopsy-free (blood) circulating tumor cell DNA assay | Oncotarget | 26848768 | yzed for 54 genes via NGS in circulating tumor, cell-free DNA. RESULTS Analysis of control samples from healthy persons During the technology development process, 79 healthy normal controls (source: AllCells, http://www.allcells.com) were tested and | Full Paper |
2016 | Li, Y;Wang, Y;Li, L;Kong, R;Pan, S;Ji, L;Liu, H;Chen, H;Sun, B; | Hyperoside induces apoptosis and inhibits growth in pancreatic cancer via Bcl-2 family and NF-κB signaling pathway both in vitro and in vivo | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | 26676634 | Human umbilical vein endothelial cell line (HUVEC) was obtained from all cells (Shanghai, China) and were cultured in complete medium (HUVEC-004, supplied by AllCells). All cells Save | Full Paper |
2016 | Aizman, I;Holland, WS;Yang, C;Bates, D; | αSMA Expression in Large Colonies of Colony-Forming Units-Fibroblast as an Early Predictor of Bone Marrow MSC Expandability | Cell medicine | 28003933 | The three lots that were produced from BM aspirates obtained at AllCells and, coincidentally, from older donors (see Materials and Methods) were all in the slow-growing group; however Save | Full Paper |
2016 | Liu, W;Lu, Y;Chai, X;Liu, X;Zhu, T;Wu, X;Fang, Y;Liu, X;Zhang, X; | Antitumor activity of TY-011 against gastric cancer by inhibiting Aurora A, Aurora B and VEGFR2 kinases | Journal of experimental & clinical cancer research : CR | 27887633 | Human umbilical vein endothelial cell (HUVEC) obtained from Allcells (Emeryville, CA, USA) were kept in HUVEC basic medium supplemented with 10% FBS (Allcells, Emeryville, CA, Save | Full Paper |
2016 | Han, X;Medeiros, LJ;Zhang, YH;You, MJ;Andreeff, M;Konopleva, M;Bueso-Ramos, CE; | High Expression of Human Homologue of Murine Double Minute 4 and the Short Splicing Variant, HDM4-S, in Bone Marrow in Patients With Acute Myeloid Leukemia or Myelodysplastic Syndrome | Clinical lymphoma, myeloma & leukemia | 27155969 | cDNAs from purified normal CD33+ bone marrow myeloid cells, CD34+ hematopoietic progenitor cells, CD71+ normoblasts, and CD19+ circulating peripheral blood B cells were purchased from AllCells, LLC (Emeryville, CA) for reverse transcriptase-polymeras | Full Paper |
2016 | Ali, S; | Cell Line Cross-Contamination and Accidental Co-Culture | Journal of Stem Cell Research & Therapeutics | a https://www.abmgood.com/ Custom-Cell-Authentication-Service.html STR Profiling Analytical Biological Services Inc, USA http://www.absbio.com/products /cellQC/celllineauthen.html STR profiling AllCells, California www.allcells.com - Asteran | Full Paper | |
2016 | Dou, W;Cheung, S;Gao, C;Xu, C;Xu, L;Wei, J; | Detecting table clones and smells in spreadsheets | Proceedings of the 2016 24th ACM SIGSOFT International Symposium on Foundations of Software Engineering | TableCheck's clone detection algorithm (Algorithm 1) takes the lookup table and all cells (allCells) Then all cells in this group are removed from allCells (Lines 14-18). (5) The algorithm Save | Full Paper | |
2016 | DeWitt, M;Magis, W;Bray, N;Wang, T;Berman, J;Urbinati, F;Muñoz, D;Kohn, D;Walters, M;Carroll, D;Martin, D;Corn, J; | Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein Complex | bioRxiv | Cryopreserved WT human mobilized peripheral blood CD34+ HSPCs were purchased from Allcells, Inc. SCD CD34+ HSPCs were prepared by Allcells Inc. from whole blood obtained Save | Full Paper | |
2016 | Meader, ES; | Understanding the role of miRNA expression in the differentiation of haematopoietic stem cells from pluripotent stem cells | Thesis | not been released in spite of several requests, but the sequences of the miRNAs tested are shown in Table 2.4. Small nuclear RNAs RNU44 and RNU48 were chosen as internal controls as they are known to be stably and abundantly expressed across differen | Full Paper | |
2016 | Tran, R; | A microfluidics-based paradigm for clinical lentivector gene transfer | Thesis | Frozen human pan T cells were purchased from AllCells (AllCells, LLC., Alameda, CA, USA). Cells were thawed following the AllCells thawing protocol and allowed to recover in RPMI 1640 media supplemented with 10% FBS, L-Glutamine, 25 mM HEPES, and 1% | Full Paper | |
2016 | Abu Samra, DBK; | Molecular Characterization of the Interactions between Vascular Selectins and Glycoprotein Ligands on Human Hematopoietic Stem/Progenitor Cells | Thesis | bone marrow (CD34+ -bone marrow), AML derived bone marrow (from patients with newly diagnosed or relapsed AML; AML bone marrow-CD34+ ) or the mononuclear fraction of whole bone marrow were purchased from ALL CELLS (Alameda, CA, USA; www.allcells.com) | Full Paper | |
2016 | Ma, L; | Targeting Drug Resistance in Chronic Myeloid Leukemia: A Dissertation | Thesis | in FBS + 10% DMSO, frozen slowly overnight, and then transferred to liquid nitrogen for long-term storage. All samples were provided in a de-identified manner. Samples from normal donors were purchased from a commercial vendor (AllCells or Lonza) | Full Paper | |
2016 | Liu, X; | Functional and structural study of the AHI-1 SH3 domain, characterization of the BCR-ABL-AHI-1-Dynamin-2 protein complex and investigation of oncogenic roles of dynamin-2 in chronic myeloid leukemia | Thesis | Primary CML samples were obtained from newly-diagnosed CP-CML patients before any TKI intervention, and BM cells were obtained from health donors (ALLCELLS, Alameda, CA). The subsequent IM-responders were classified based on the European Leukemia Net | Full Paper | |
2016 | Ferreira, L; | Transcriptional Control of Maternal-Fetal Immune Tolerance | Thesis | Primary CD4+ T cells were isolated from peripheral blood (Leukopacs, MGH) using RosetteSep CD4+ T cell enrichment cocktail (STEMCELL Technologies). CD34+ cells from G-CSF mobilized peripheral blood were purchased from AllCells | Full Paper | |
2016 | Coronado, RE; | The effect of decellularized liver matrix on stem cell differentiation into hepatocyte-like cells | Thesis | Bone Marrow Derived Mesenchymal Stromal/Stem Cells: Bone marrow derived mesenchymal stem cells (BM-MSC) were isolated from whole bone marrow aspirates purchased from AllCells Alameda, CA from donors less than 30 years old. The isolation protocol was | Full Paper | |
2016 | Gangolli, R; | A novel biomimetic scaffold for guided tissue regeneration of the pulp-dentin complex | Thesis | DPSC from AllCells at Passage P5 were used for the study. DPSC-P5 were cultured on T175 flasks using proliferation media with MEM Alpha, 15% FBS and 1% Pen-strep which had been filtered through a 0.2 um filter flask. Culture conditions were maintaine | Full Paper | |
2016 | Bjornson-Hooper, ZB; | Cross-Species Immune System Atlas | Thesis | Venous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human subjects research) or from volunteers from the Stanford community under an IRB-approved protocol. Baboon (Papio hamadryas anubis x Papio ha | Full Paper | |
2016 | Varma, D; | Development of a Redox-Crosslinked Cellulose-Based Hydrogel for Nucleus Pulposus Replacement and Repair | Thesis | hMSCs were isolated from adult human bone marrow (AllCells, Emeryville, CA) via enriched density gradient centrifugation as previously described (Gupta et al., 2011; Gupta and Nicoll, 2014b). Briefly, an hMSC Enrichment Cocktail (StemCell Technologie | Full Paper | |
2016 | Sivajothi, SK; | Comparative properties of stromal cells and their role in tissue regeneration | Thesis | 2.1 Apheresis Products Non-mobilized PBPC and G-CSF mobilized PBPC were purchased from Allcells LLC (Emeryville, CA): 2.2 Cells and Cell Culture Human bone marrow MSCs were isolated by differential adhesion from bonemarrow aspirate obtained from huma | Full Paper | |
2016 | Fragiadakis, GK; | Profiling Human Immune Variation at the Systems-Level in Clinical Contexts and at Steady-State | Thesis | Blood Venous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human subjects research) or from volunteers from the Stanford community under an IRB-approved protocol. Antibodies Purified antibodies were | Full Paper | |
2016 | Fried, ES; | Applications of Proteolipobeads in Biomimetic Cellular Interactions And Lipid Phase Separation | Thesis | N-Diisopropylethylamine (DIEA), piperidine, carboxymethylcellulose (CMC), and methacrylic anhydride were purchased from Sigma Aldrich (St. Louis, MO). Human mesenchymal stem cells (MSCs) isolated from bone marrow extracted from a 24-year-old male wer | Full Paper | |
2016 | Lucera, MB; | Lysine Acetylation and Small Molecule Epigenetic Inhibition Reveal Novel Mechanisms Controlling Cellular Susceptibility to HIV-1 Infection | Thesis | CD4+ T cells were obtained by leukopheresis from ALLCELLS, LLC. All subjects were healthy control donors negative for HBV, HCV, and HIV. PBMCs were isolated by ficoll gradient separation while CD4+ T cells were purified by adding RosetteSep CD4+ T ce | Full Paper | |
2016 | Milford, TA; | Comparative Studies of TSLP and IL-7 in Normal Early Human Neonatal and Adult B Cell Development | Thesis | CB was collected according to guidelines approved by the Institutional Review Boards at Children’s Hospital Los Angeles and Loma Linda University. Adult human BM was purchased from AllCells, LLC, Berkeley, CA; the National Disease Research Interchang | Full Paper | |
2016 | Richter, M;Saydaminova, K;Yumul, R;Krishnan, R;Liu, J;Nagy, EE;Singh, M;Izsvák, Z;Cattaneo, R;Uckert, W;Palmer, D;Ng, P;Haworth, KG;Kiem, HP;Ehrhardt, A;Papayannopoulou, T;Lieber, A; | In vivo transduction of primitive mobilized hematopoietic stem cells after intravenous injection of integrating adenovirus vectors | Blood | 27554082 | Human CD34+-enriched cells from G-CSF mobilized normal donors were obtained from the Fred Hutchinson Cancer Research Center Cell Processing Core Facility and cultured as described previously.8 Colony-forming unit (CFU) assays were performed using Col | Full Paper |
2016 | Castillo, J;Raisner, R;Gascoigne, K;Dos Santos, G;Clarke, E;Huang, A;Lackner, M;Mounir, Z; | Abstract 4516: Evaluating the chromatin state as a predictive biomarker for BET inhibitor sensitivity in hematological malignancies | Cancer Research | 1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++2ReachBio, WA.++2ReachBio, WA.++1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++1Genentech, South San Franci | Full Paper | |
2016 | Ferguson, GD;Delgado, M;Plantevin-Krenitsky, V;Jensen-Pergakes, K;Bates, RJ;Torres, S;Celeridad, M;Brown, H;Burnett, K;Nadolny, L;Tehrani, L;Packard, G;Pagarigan, B;Haelewyn, J;Nguyen, T;Xu, L;Tang, Y;Hickman, M;Baculi, F;Pierce, S;Miyazawa, K;Jackson, P;Chamberlain, P;LeBrun, L;Xie, W;Bennett, B;Blease, K; | A Novel Triazolopyridine-Based Spleen Tyrosine Kinase Inhibitor That Arrests Joint Inflammation | PloS one | 26756335 | ature in the dark. Fluorescence readings at 460 nm and 530 nm were taken using the Flexstation II (Molecular Devices). Myeloid colony forming assays to assess Jak2 cellular activity were conducted at ReachBio (Seattle, WA). Ramos NFAT β-lactamase re | Full Paper |
2016 | Hariharan, S; | STUDY OF GROWTH OF STRESS ERYTHROID PROGENITORS FROM PERIPHERAL BLOOD MONONUCLEAR CELLS IN MURINE AND HUMAN ANEMIC MODEL SYSTEMS | Thesis | The control Peripheral Blood samples were obtained from ReachBio Labs. The PBMCs were then isolated from peripheral blood using Histopaque 1077 using density gradient. The Save | Full Paper | |
2016 | Holzapfel, N; | Investigating the Role of the RNA-Binding Protein MUSASHI-2 (MSI2) in Normal Hematopoiesis and Leukemia | Thesis | AML*CFU%assays%were%performed%in%semi*solid%ColonyGel%media%(Human%Complete% Media% 1102;% reachBio)% with% flow*sorted% GFP+%MSI2% knockdown% AML% cells.% 10000% GFP+%cells%were%plated%in%duplicate%in%each%well%of%a%24*well%plate%and%loose%colonies% | Full Paper | |
2015 | Sun, LL;Ellerman, D;Mathieu, M;Hristopoulos, M;Chen, X;Li, Y;Yan, X;Clark, R;Reyes, A;Stefanich, E;Mai, E;Young, J;Johnson, C;Huseni, M;Wang, X;Chen, Y;Wang, P;Wang, H;Dybdal, N;Chu, YW;Chiorazzi, N;Scheer, JM;Junttila, T;Totpal, K;Dennis, MS;Ebens, AJ; | Anti-CD20/CD3 T cell-dependent bispecific antibody for the treatment of B cell malignancies | Sci Transl Med | 25972002 | We have obtained 40 ml of whole blood collected in Streck tubes, separated into plasma and extracted cfRNA samples from two Stage IV Prostate cancer patients and three healthy controls from Conversant Bio and generated cfRNA sequencing data with UMIs | Full Paper |
2015 | Li, L;Lyu, X;Hou, C;Takenaka, N;Nguyen, HQ;Ong, CT;Cubeñas-Potts, C;Hu, M;Lei, EP;Bosco, G;Qin, ZS;Corces, VG; | Widespread rearrangement of 3D chromatin organization underlies polycomb-mediated stress-induced silencing | Mol. Cell | 25818644 | We thank Drs. John T. Lis for providing antibodies against RNAPII, and Vincent Pirrotta and Richard Jones for providing antibodies against Pc. We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology, and special | Full Paper |
2015 | Bhate, A;Parker, DJ;Bebee, TW;Ahn, J;Arif, W;Rashan, EH;Chorghade, S;Chau, A;Lee, JH;Anakk, S;Carstens, RP;Xiao, X;Kalsotra, A; | ESRP2 controls an adult splicing programme in hepatocytes to support postnatal liver maturation | Nat Commun | 26531099 | ... RI digested amplified PCR products for Esrp2 cDNA in pDP19 vector (Ambion). Sense and anti-sense RNA strands were generated as previously described43. In situ hybridization for Esrp2 was performed by Phylogeny Inc. (Columbus, Ohio.) The C57BL/6 m | Full Paper |
2015 | Weckselblatt, B;Hermetz, KE;Rudd, MK; | Unbalanced translocations arise from diverse mutational mechanisms including chromothripsis | Genome Res. | 26070663 | We used Agilent SureSelect Target Enrichment to pull down 40-kb regions around breakpoints fine-mapped by custom array CGH. SureSelect followed by Illumina HiSeq sequencing was performed at HudsonAlpha Genomic Services Laboratory, and sequence analys | Full Paper |
2015 | Jagannathan, S;Abdel-Malek, MA;Malek, E;Vad, N;Latif, T;Anderson, KC;Driscoll, JJ; | Pharmacologic screens reveal metformin that suppresses GRP78-dependent autophagy to enhance the anti-myeloma effect of bortezomib | Leukemia | 26108695 | Eagle's medium. Bone marrow aspirates were obtained after approval institutional review board or from ConversantBio (Huntsville, AL, USA) and CD138 + cells then isolated (Miltenyi Biotec, San Diego, CA, USA). Side population | Full Paper |
2015 | Jagannathan, S;Vad, N;Vallabhapurapu, S;Vallabhapurapu, S;Anderson, KC;Driscoll, JJ; | MiR-29b replacement inhibits proteasomes and disrupts aggresome+autophagosome formation to enhance the antimyeloma benefit of bortezomib | Leukemia | 25234165 | (c) Effect of control or miR-29b on myeloma patient tumor cells. CD138 + PCs from bone marrow (BM; ConversantBio, Huntsville, AL, USA) were isolated using microbead-positive selection (Miltenyi Biotec, Auburn, CA, USA) | Full Paper |
2015 | Blume-Jensen, P;Berman, DM;Rimm, DL;Shipitsin, M;Putzi, M;Nifong, TP;Small, C;Choudhury, S;Capela, T;Coupal, L;Ernst, C;Hurley, A;Kaprelyants, A;Chang, H;Giladi, E;Nardone, J;Dunyak, J;Loda, M;Klein, EA;Magi-Galluzzi, C;Latour, M;Epstein, JI;Kantoff, P;Saad, F; | Development and clinical validation of an in situ biopsy-based multimarker assay for risk stratification in prostate cancer | Clin. Cancer Res. | 25733599 | To develop a robust assay, multiple institutions were recruited representing typical North American patient cohorts (Table 1): Urology Austin, Chesapeake Urology Associates, Cleveland Clinic, Michigan Urology, and Folio Biosciences. Biopsy sample inc | Full Paper |
2015 | Garcia-Manero, G;Khoury, HJ;Jabbour, E;Lancet, J;Winski, SL;Cable, L;Rush, S;Maloney, L;Hogeland, G;Ptaszynski, M;Calvo, MC;Bohannan, Z;List, A;Kantarjian, H;Komrokji, R; | A phase I study of oral ARRY-614, a p38 MAPK/Tie2 dual inhibitor, in patients with low or intermediate-1 risk myelodysplastic syndromes | Clin. Cancer Res. | 25480830 | ... s (Aurora, CO) proprietary CellMap customized algorithm for p-p38 and CC3, respectively. Age-defined FFPE bone marrow biopsies from apparently healthy subjects (60–74 years old) were obtained from Folio Biosciences (Columbus, OH). Plasma was iso | Full Paper |
2015 | Grabek, KR;Diniz Behn, C;Barsh, GS;Hesselberth, JR;Martin, SL; | Enhanced stability and polyadenylation of select mRNAs support rapid thermogenesis in the brown fat of a hibernator | Elife | 25626169 | Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, United States.++Department of Applied Math and Statistics, Colorado School of Mines, Golden, United States.++Department of Research, HudsonAlpha Institut | Full Paper |
2015 | Li, C;Gowan, S;Anil, A;Beck, BH;Thongda, W;Kucuktas, H;Kaltenboeck, L;Peatman, E; | Discovery and validation of gene-linked diagnostic SNP markers for assessing hybridization between Largemouth bass (Micropterus salmoides) and Florida bass (M. floridanus) | Mol Ecol Resour | 25047482 | ; of 110-140 nm. After KAPA quantitation and dilution, based on included DNA standards (1-6), the libraries were sequenced in a single lane on an Illumina HiSeq 2000 instrument with 100 bp paired‐end (PE) reads at HudsonAlpha Genomic Services Lab (H | Full Paper |
2015 | Nguyen, D;Rubinstein, L;Takebe, N;Miele, L;Tomaszewski, JE;Ivy, P;Doroshow, JH;Yang, SX; | Notch1 phenotype and clinical stage progression in non-small cell lung cancer | J Hematol Oncol | 25653136 | Lung cancer tissue microarrays and clinicopathological data were obtained from Cybrdi, Incorporation (Rockville, MD), and FOLIO Biosciences (Powell, OH). Approval of the biomarker study on de-identified human tissues was obtained from the Office of H | Full Paper |
2015 | Huynh, H;Hao, HX;Chan, SL;Chen, D;Ong, R;Soo, KC;Pochanard, P;Yang, D;Ruddy, D;Liu, M;Derti, A;Balak, MN;Palmer, MR;Wang, Y;Lee, BH;Sellami, D;Zhu, AX;Schlegel, R;Huang, A; | Loss of Tuberous Sclerosis Complex 2 (TSC2) Is Frequent in Hepatocellular Carcinoma and Predicts Response to mTORC1 Inhibitor Everolimus | Mol. Cancer Ther. | 25724664 | HCC cell lines and patient-derived xenografts were collected, fixed with formalin and processed for paraffin embedding. HCC tissue microarrays were purchased from BioChain (catalog # Z7020056 and Z7020057, unrelated HCC TMAs) and FolioBio (catalog# A | Full Paper |
2015 | Mezache, L;Paniccia, B;Nyinawabera, A;Nuovo, GJ; | Enhanced expression of PD L1 in cervical intraepithelial neoplasia and cervical cancers | Mod. Pathol. | 26403783 | and cervical cancers Louisa Mezache 1 Bernard Paniccia 1 Angelique Nyinawabera 1 Gerard Nuovo 1,2 Phylogeny Inc. 1 , Powell, Ohio and OSU Comprehensive Cancer Center 2 , Columbus Ohio Page 2. 2 - Mezache et al Direct all correspondence to: Gerard J N | Full Paper |
2015 | Shen, J;Putt, KS;Visscher, DW;Murphy, L;Cohen, C; | Assessment of folate receptor-β expression in human neoplastic tissues | Oncotarget | ... from US Biomax (Rockville, MD), and v) a breast tumor microarray (ARY-HH0056), a brain glioma tumor microarray (ARY-HH0138) and an endometrial carcinoma progression tumor microarray (ARY-HH0211) from Folio Biosciences (Columbus, OH). Oregon green | Full Paper | |
2015 | Azad, AK;Rajaram, MV;Metz, WL;Cope, FO;Blue, MS;Vera, DR;Schlesinger, LS; | γ-Tilmanocept, a New Radiopharmaceutical Tracer for Cancer Sentinel Lymph Nodes, Binds to the Mannose Receptor (CD206) | J. Immunol. | 26202986 | We thank Phylogeny, Inc. (Powell, OH) for sectioning and processing the lymph node tissues. We also acknowledge the support of the Campus Microscopy and Imaging Facility of The Ohio State University (Columbus, OH) for confocal microscopy. | Full Paper |
2015 | Waight, JD;Takai, S;Marelli, B;Qin, G;Hance, KW;Zhang, D;Tighe, R;Lan, Y;Lo, KM;Sabzevari, H;Hofmeister, R;Wilson, NS; | Cutting edge: epigenetic regulation of Foxp3 defines a stable population of CD4+ regulatory T cells in tumors from mice and humans | J. Immunol. | 25548231 | Mouse tumor cells were isolated as described previously (12). Human tumor specimens were collected before therapy and purchased from Conversant Bio. Healthy donor PBMCs (Research Blood Components) were isolated using Ficoll-Paque density gradient. Sa | Full Paper |
2015 | Li, Y;Kocot, KM;Schander, C;Santos, SR;Thornhill, DJ;Halanych, KM; | Mitogenomics reveals phylogeny and repeated motifs in control regions of the deep-sea family Siboglinidae (Annelida) | Mol. Phylogenet. Evol. | 25721539 | Mitogenomics reveals phylogeny and repeated motifs in control regions of the deep-sea family Siboglinidae (Annelida) | Full Paper |
2015 | Cheng, A;Hernandez, N;Li, M;San Jose Hinahon, C;Magdaleno, S; | Abstract 4755: Maximize genomic analysis through dual recovery of DNA and RNA from the same clinical sample | Molecular and Cellular Biology | variant, indel data, RNA fusion detection and expression data. MATERIALS AND METHODS FFPE surgical resection and FNA blocks were purchased from Folio Biosciences. Age of blocks ranged from 1992- 2011 with various tumor stages. One 4um section mounted | Full Paper | |
2015 | Cunningham, CB;Ji, L;Wiberg, RA;Shelton, J;McKinney, EC;Parker, DJ;Meagher, RB;Benowitz, KM;Roy-Zokan, EM;Ritchie, MG;Brown, SJ;Schmitz, RJ;Moore, AJ; | The Genome and Methylome of a Beetle with Complex Social Behavior, Nicrophorus vespilloides (Coleoptera: Silphidae) | Genome Biol Evol | 26454014 | Testing for conserved and novel mechanisms underlying phenotypic evolution requires a diversity of genomes available for comparison spanning multiple independent lineages. For example, complex social behavior in insects has been investigated primaril | Full Paper |
2015 | Yamaguchi, SI;Ueki, A;Sugihara, E;Onishi, N;Yaguchi, T;Kawakami, Y;Horiuchi, K;Morioka, H;Matsumoto, M;Nakamura, M;Muto, A;Toyama, Y;Saya, H;Shimizu, T; | Synergistic antiproliferative effect of imatinib and adriamycin in platelet-derived growth factor receptor-expressing osteosarcoma cells | Cancer Sci. | 25940371 | A lung tissue section was randomly obtained from each lobe. Human OS tissue array. An array of human OS specimens was obtained from Folio Biosciences (Powell, OH, USA) and was subjected to immunohistochemical staining for PDGFRβ | Full Paper |
2015 | Zemskova, MY;Song, JH;Cen, B;Cerda-Infante, J;Montecinos, VP;Kraft, AS; | Regulation of prostate stromal fibroblasts by the PIM1 protein kinase | Cell. Signal. | 25451079 | scores. P values were calculated by t test and represent the probability of no difference between PIM1 positive cells in the stroma of normal and cancer tissues. (C) IHC staining of PIM1 using a TMA (Folio Bioscience). PIM1 | Full Paper |
2015 | Ergin, A;Großerüschkamp, F;Theisen, O;Gerwert, K;Remiszewski, S;Thompson, CM;Diem, M; | A method for the comparison of multi-platform spectral histopathology (SHP) data sets | Analyst | 25664352 | M. Miljković, et al., Spectral Cytopathology: new aspects of data collection, manipulation and confounding effects, Analyst, 2013, 138, 3975-3982 RSC . foliobio.com. Deparaffin Protocol. Available from: http://foliobio.com/media/pdf/Deparaffin%20prot | Full Paper |
2015 | Steffen, MM;Belisle, BS;Watson, SB;Boyer, GL;Bourbonniere, RA;Wilhelm, SW; | Metatranscriptomic evidence for co-occurring top-down and bottom-up controls on toxic cyanobacterial communities | Appl. Environ. Microbiol. | 25662977 | ... using the Epicentre Ribo-Zero rRNA removal kit (Epicentre, Madison, WI, USA). Secondary quality analysis and sequencing were done on the Illumina HiSeq platform for 50-bp paired-end sequencing by the HudsonAlpha Genomic Services Laboratory (Hunts | Full Paper |
2015 | Van Bortle, K;Peterson, AJ;Takenaka, N;O'Connor, MB;Corces, VG; | CTCF-dependent co-localization of canonical Smad signaling factors at architectural protein binding sites in D. melanogaster | Cell Cycle | 26125535 | llumina adaptors (Illumina Cat# PE-102-1001) were titrated according to prepared DNA ChIP sample concentration and ligated with T4 ligase (NEB Cat# M0202S). Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (NE | Full Paper |
2015 | Ritchie, SA;Chitou, B;Zheng, Q;Jayasinghe, D;Jin, W;Mochizuki, A;Goodenowe, DB; | Pancreatic cancer serum biomarker PC-594: Diagnostic performance and comparison to CA19-9 | World J. Gastroenterol. | 26074698 | MATERIALS AND METHODS. Sample cohort. PaC (n = 84) and control (n = 99) serum samples were obtained from Conversant Bio (www.conversantbio.com). All patients signed informed consents, and samples were collected | Full Paper |
2015 | Premkumar, DR;Jane, EP;Pollack, IF; | Cucurbitacin-I inhibits Aurora kinase A, Aurora kinase B and survivin, induces defects in cell cycle progression and promotes ABT-737-induced cell death in a caspase-independent manner in malignant human glioma cells | Cancer Biol. Ther. | 25482928 | We also obtained primary glioma cells from Conversant Biologics (Huntsville, AL). Established malig- nant human glioma cell lines such as T98G, A172 and LN229 were obtained from the American Type Culture Collection (Manassas, VA) | Full Paper |
2015 | Tehranchian, P;Norsworthy, JK;Nandula, V;McElroy, S;Chen, S;Scott, RC; | First report of resistance to acetolactate-synthase-inhibiting herbicides in yellow nutsedge (Cyperus esculentus): confirmation and characterization | Pest Manag. Sci. | 25307777 | To identify potential target-site mutations, massively parallel sequencing using the Illumina HiSeq (Illumina, San Diego, CA; http://www.illumina.com/) platform was utilized in lieu of traditional short-read capillary sequencing using the polymerase | Full Paper |
2015 | Campos, AH;Schreeder, M;Parry-Jones, A;Abdelhafiz, AS;Larson, D;Pruetz, B;Geddes, T;Salman, A;Lazaris, A; | Addressing the Challenge of Financial Sustainability in Biobanking | Biopreserv Biobank | 26697907 | Marshall Schreeder. Chief Executive Officer, Co-Founder, Conversant Bio, Huntsville, Alabama Conversant Bio, Huntsville, Alabama. Conversant Bio was founded in 2006 because the founders knew that the drug discovery research model was broken | Full Paper |
2015 | Chen, S;McElroy, JS;Flessner, ML;Dane, F; | Utilizing next-generation sequencing to study homeologous polymorphisms and herbicide-resistance-endowing mutations in Poa annua acetolactate synthase genes | Pest Manag. Sci. | 25180862 | Detection of single nucleotide polymorphisms (SNPs) related to herbicide resistance in non-model polyploid weed species is fraught with difficulty owing to the gene duplication and lack of reference sequences. Our research seeks to overcome these obs | Full Paper |
2015 | Cui, J;Xu, J;Zhang, S;Wang, K;Jiang, Y;Mahboob, S;Al-Ghanim, KA;Xu, P; | Transcriptional Profiling Reveals Differential Gene Expression of Amur Ide (Leuciscus waleckii) during Spawning Migration | Int J Mol Sci | 26096003 | ... nt Technologies, Santa Clara, CA, USA). Equal amounts of high quality RNA samples from the liver were then pooled for RNA-Seq analysis. RNA-Seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2015 | Baker, GM;Murphy, T;Block, T;Nguyen, D;Lynch, FJ; | Development and validation of an immunohistochemistry assay to assess glucocorticoid receptor expression for clinical trials of mifepristone in breast cancer | Cancer Manag Res | 26673410 | Tiffany Murphy. 2 QualTek Molecular Laboratories, Newtown, PA, USA. Find articles by Tiffany Murphy. Thaddeus Block Dat Nguyen. 3 Corcept Therapeutics, Menlo Park, CA, USA. Find articles by Dat Nguyen. Frank J Lynch. 2 QualTek Molecular Laboratorie | Full Paper |
2015 | Zisis, K;Piper, M;Henderson, MK; | The 2015 ISBER Annual Meeting & Exhibits in Phoenix, Arizona, USA: bridging the canyon-connecting biobank communities through innovations in global health, research and environmental preservation | Biopreserv Biobank | 25686051 | This year's session focused on the business aspects of biobank planning, and included Marshall Schreeder's (ConversantBIO) perspective from a commer- cial biobank and Clive Green's (AstraZeneca) business planning for a pharmaceutical biobank | Full Paper |
2015 | Zhao, H;Li, C;Beck, BH;Zhang, R;Thongda, W;Davis, DA;Peatman, E; | Impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings, Ictalurus punctatus | Fish Shellfish Immunol. | 26164837 | RNA quality of each sample was measured on an Agilent 2100 Bioanalyzer using the RNA Nano Bioanalysis chip. RNA-seq library preparation was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) | Full Paper |
2015 | Zhu, J;Li, C;Ao, Q;Tan, Y;Luo, Y;Guo, Y;Lan, G;Jiang, H;Gan, X; | Trancriptomic profiling revealed the signatures of acute immune response in tilapia (Oreochromis niloticus) following Streptococcus iniae challenge | Fish Shellfish Immunol. | 26117728 | ; For each timepoint, equal amounts of RNA from the three replicates were pooled for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) | Full Paper |
2015 | Foster, KA;Jane, EP;Premkumar, DR;Morales, A;Pollack, IF; | NVP-BKM120 potentiates apoptosis in tumor necrosis factor-related apoptosis-inducing ligand-resistant glioma cell lines via upregulation of Noxa and death receptor 5 | Int. J. Oncol. | 26044191 | U87 was obtained from American Type Culture Collection (Manasas, VA, USA) and LNZ308 was kindly provided by Dr Nicolas de Tribolet (Switzerland). We obtained primary GBM cells from Conversant Biologics (Huntsville, AL, USA) | Full Paper |
2015 | Baxter, A;Thain, S;Banerjee, A;Haswell, L;Parmar, A;Phillips, G;Minet, E; | Targeted omics analyses, and metabolic enzyme activity assays demonstrate maintenance of key mucociliary characteristics in long term cultures of reconstituted human airway epithelia | Toxicol In Vitro | 25863282 | The 180 PBS was collected and stored at A80 °C. Sputum from 10 non- 181 smokers (Sofia Bio, Sofia, Bulgaria, study code SB-SC_v1) and 182 MucilAir™ ASL were thawed, centrifuged and filtered using a 183 0.2 lm filter plate (PALL Corporation, Port Wash | Full Paper |
2015 | Marshall, DC;Lyman, SK;McCauley, S;Kovalenko, M;Spangler, R;Liu, C;Lee, M;O'Sullivan, C;Barry-Hamilton, V;Ghermazien, H;Mikels-Vigdal, A;Garcia, CA;Jorgensen, B;Velayo, AC;Wang, R;Adamkewicz, JI;Smith, V; | Selective Allosteric Inhibition of MMP9 Is Efficacious in Preclinical Models of Ulcerative Colitis and Colorectal Cancer | PLoS ONE | 25961845 | ... h as CRC. Materials and Methods Tissues Fresh-frozen (FF) and formalin fixed and paraffin embedded (FFPE) human tissues were obtained from Cureline, Inc. (Burlingame, CA), Asterand (Detroit, MI), or Folio Biosciences (Powell, OH). Recombinant M | Full Paper |
2015 | Hoek, KL;Samir, P;Howard, LM;Niu, X;Prasad, N;Galassie, A;Liu, Q;Allos, TM;Floyd, KA;Guo, Y;Shyr, Y;Levy, SE;Joyce, S;Edwards, KM;Link, AJ; | A cell-based systems biology assessment of human blood to monitor immune responses after influenza vaccination | PLoS ONE | 25706537 | Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, 37232, United States of America.++Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, 37232, United States | Full Paper |
2015 | Kalinsky, K;Mayer, JA;Xu, X;Pham, T;Wong, KL;Villarin, E;Pircher, TJ;Brown, M;Maurer, MA;Bischoff, FZ; | Correlation of hormone receptor status between circulating tumor cells, primary tumor, and metastasis in breast cancer patients | Clin Transl Oncol | 25613123 | Peripheral blood was collected under appropriate third party institution review board approved protocols (Columbia University Medical Center, AdeptBio, ConversantBIO and BioOptions) and delivered to Biocept's CLIA/CAP accredited laboratory | Full Paper |
2015 | Wang, Z;Lebron, JA;Wolf, JJ; | Reliable quantification of mRNA in archived formalin-fixed tissue with or without paraffin embedding | J Pharmacol Toxicol Methods | 25280797 | quantify mRNA in archived FFPE and prolonged formalin-fixed tissue. 2. Methods. 2.1. Samples. Four human FFPE liver samples were purchased from Folio Biosciences. Two mouse FFPE liver samples and two mouse formalin | Full Paper |
2015 | Rokyta, DR;Wray, KP;McGivern, JJ;Margres, MJ; | The transcriptomic and proteomic basis for the evolution of a novel venom phenotype within the Timber Rattlesnake (Crotalus horridus) | Toxicon | 25727380 | Sequencing and nonnormalized cDNA library preparation for our two adults were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially a | Full Paper |
2015 | Pawar, H;Srikanth, SM;Kashyap, MK;Sathe, G;Chavan, S;Singal, M;Manju, HC;Kumar, KV;Vijayakumar, M;Sirdeshmukh, R;Pandey, A;Prasad, TS;Gowda, H;Kumar, RV; | Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell Carcinoma | ScientificWorldJournal | 26788548 | The ESCC tumors varied from Grade I to Grade III and were obtained from patients in the age group of 36 to 78 years and (ii) FolioBio (Powell, OH; Catalog number ARY-HH0091) consisting of 40 ESCC cases with matched adjacent normal esophageal epitheli | Full Paper |
2015 | Krönke, J;Fink, EC;Hollenbach, PW;MacBeth, KJ;Hurst, SN;Udeshi, ND;Chamberlain, PP;Mani, DR;Man, HW;Gandhi, AK;Svinkina, T;Schneider, RK;McConkey, M;Järås, M;Griffiths, E;Wetzler, M;Bullinger, L;Cathers, BE;Carr, SA;Chopra, R;Ebert, BL; | Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDS | Nature | 26131937 | uced cells in each condition was assessed after 5 days by flow cytometry. Control cord-blood CD34+ cells were isolated as above. Adult bone marrow CD34+ cells were purchased as single-donor lots from AllCells (Alameda, CA). The number of replicates f | Full Paper |
2015 | Hendel, A;Bak, RO;Clark, JT;Kennedy, AB;Ryan, DE;Roy, S;Steinfeld, I;Lunstad, BD;Kaiser, RJ;Wilkens, AB;Bacchetta, R;Tsalenko, A;Dellinger, D;Bruhn, L;Porteus, MH; | Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells | Nature biotechnology | 26121415 | Cas9-encoding plasmid. Mobilized human peripheral blood CD34+ HSPCs were purchased from AllCells and thawed according to manufacturer’s instructions. CD34+ HSPCs were maintained in X-VIVO 15 (Lonza) supplemented with SCF (100 ng/ml), TPO (100 ng/ml), | Full Paper |
2015 | Kotini, AG;Chang, CJ;Boussaad, I;Delrow, JJ;Dolezal, EK;Nagulapally, AB;Perna, F;Fishbein, GA;Klimek, VM;Hawkins, RD;Huangfu, D;Murry, CE;Graubert, T;Nimer, SD;Papapetrou, EP; | Functional analysis of a chromosomal deletion associated with myelodysplastic syndromes using isogenic human induced pluripotent stem cells | Nature biotechnology | 25798938 | CB CD34 + cells were purchased from AllCells. Two to four shRNAs against each of the four genes HIPK2, ATP6V0E2, LUC7L2 and EZH2 and one scramble sequence were inserted in Save | Full Paper |
2015 | Levine, JH;Simonds, EF;Bendall, SC;Davis, KL;Amir, el-AD;Tadmor, MD;Litvin, O;Fienberg, HG;Jager, A;Zunder, ER;Finck, R;Gedman, AL;Radtke, I;Downing, JR;Pe'er, D;Nolan, GP; | Data-Driven Phenotypic Dissection of AML Reveals Progenitor-like Cells that Correlate with Prognosis | Cell | 26095251 | For healthy adult controls, cryopreserved healthy BMMCs were purchased from AllCells (Emeryville, CA). All human samples were obtained with informed consent in compliance with Save | Full Paper |
2015 | Moraga, I;Wernig, G;Wilmes, S;Gryshkova, V;Richter, CP;Hong, WJ;Sinha, R;Guo, F;Fabionar, H;Wehrman, TS;Krutzik, P;Demharter, S;Plo, I;Weissman, IL;Minary, P;Majeti, R;Constantinescu, SN;Piehler, J;Garcia, KC; | Tuning cytokine receptor signaling by re-orienting dimer geometry with surrogate ligands | Cell | 25728669 | ficolled cells (32K) from a single healthy donor (donor ID #: 6024, lot#: BM4871) were purchased from Allcells, Alameda, CA 94502. Cells were subsequently treated with DNAase. Milteny CD34 magnetic beads were used to enrich for CD34+ cells with | Full Paper |
2015 | Vierstra, J;Reik, A;Chang, KH;Stehling-Sun, S;Zhou, Y;Hinkley, SJ;Paschon, DE;Zhang, L;Psatha, N;Bendana, YR;O'Neil, CM;Song, AH;Mich, AK;Liu, PQ;Lee, G;Bauer, DE;Holmes, MC;Orkin, SH;Papayannopoulou, T;Stamatoyannopoulos, G;Rebar, EJ;Gregory, PD;Urnov, FD;Stamatoyannopoulos, JA; | Functional footprinting of regulatory DNA | Nature methods | 26322838 | Human mobilized CD34 + cells (adult) were purchased from AllCells. Small-scale mRNA transfections were performed with a BTX device (Harvard Apparatus), using the CD34 + cell Save | Full Paper |
2015 | Bunin, A;Sisirak, V;Ghosh, HS;Grajkowska, LT;Hou, ZE;Miron, M;Yang, C;Ceribelli, M;Uetani, N;Chaperot, L;Plumas, J;Hendriks, W;Tremblay, ML;Häcker, H;Staudt, LM;Green, PH;Bhagat, G;Reizis, B; | Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic Cells | Immunity | 26231120 | PBMC were isolated from healthy adult volunteers by Histopaque density gradient centrifugation; where indicated, pDCs were enriched using the Diamond pDC isolation kit (Miltenyi Biotec) to 95% purity. Human PBMC subsets for expression analysis were p | Full Paper |
2015 | Behbehani, GK;Samusik, N;Bjornson, ZB;Fantl, WJ;Medeiros, BC;Nolan, GP; | Mass Cytometric Functional Profiling of Acute Myeloid Leukemia Defines Cell-Cycle and Immunophenotypic Properties That Correlate with Known Responses to Therapy | Cancer discovery | 26091827 | Healthy control samples were obtained from Allcells using the same protocol. The exception was healthy donor sample #1; this sample was incubated for approximately 1 hour with IdU Save | Full Paper |
2015 | Casero, D;Sandoval, S;Seet, CS;Scholes, J;Zhu, Y;Ha, VL;Luong, A;Parekh, C;Crooks, GM; | Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T cell lineages | Nature immunology | 26502406 | Anonymous normal human thymi were obtained via the UCLA Translational Pathology Core Laboratory and the CHLA cardiovascular thoracic surgery department. Bone marrow (BM) was obtained from donors via ALLCELLS, Alameda, CA. All tissues were collected a | Full Paper |
2015 | Swaminathan, S;Klemm, L;Park, E;Papaemmanuil, E;Ford, A;Kweon, SM;Trageser, D;Hasselfeld, B;Henke, N;Mooster, J;Geng, H;Schwarz, K;Kogan, SC;Casellas, R;Schatz, DG;Lieber, MR;Greaves, MF;Müschen, M; | Mechanisms of clonal evolution in childhood acute lymphoblastic leukemia | Nature immunology | 25985233 | Human CD19 + cord blood B cells were obtained from AllCells. AllCells procures cord blood subsequent to receiving informed consent from its donors. We have approval to use cord Save | Full Paper |
2015 | Pandolfi, A;Stanley, RF;Yu, Y;Bartholdy, B;Pendurti, G;Gritsman, K;Boultwood, J;Chernoff, J;Verma, A;Steidl, U; | PAK1 is a therapeutic target in acute myeloid leukemia and myelodysplastic syndrome | Blood | 26170031 | Poor clinical outcome of acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) has been attributed to failure of current chemotherapeutic regimens to target leukemic Save | Full Paper |
2015 | Patel, V;Balakrishnan, K;Keating, MJ;Wierda, WG;Gandhi, V; | Expression of executioner procaspases and their activation by a procaspase-activating compound in chronic lymphocytic leukemia cells | Blood | 25538042 | Normal B lymphocytes (freshly isolated negatively selected CD19 + B cells) were purchased from AllCells, and were kept in RPMI-1640 medium with 10% fetal bovine serum (FBS). Save | Full Paper |
2015 | Iwasa, M;Miura, Y;Fujishiro, A;Fujii, S;Sugino, N;Yoshioka, S;Tamura, A;Sato, A;Yokota, A;Kito, K;Ando, A;Hirai, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T; | Bortezomib Attenuates Adhesion of B Cell Precursor Acute Lymphoblastic Lleukemia Cells to Bone Marrow Mesenchymal Stromal/Stem Cells Via Regulating SPARC Expression | Blood | We isolated human BM-MSCs from normal bone marrow samples (AllCells, Emeryville, CA). When Nalm6 cells were co-cultured with human BM-MSCs, they were prone to adhere to BM Save;The prognosis of adults with B cell precursor acute lymphoblastic leukem | Full Paper | |
2015 | Shenoy, S;Eapen, M;Wu, J;Walters, M;Levine, J;Logan, B;Gersten, I;Kamani, N; | A Multicenter Phase II Trial of Unrelated Donor Reduced Intensity Bone Marrow Transplantation for Children with Severe Sickle Cell Disease (SCURT): Results of the Blood and Marrow Transplant Clinical Trials Network (BMT CTN 0601) Study | Blood | Background: Children with severe sickle cell disease (SCD) experience organ damage, poor quality of life, and a risk of premature mortality. Allogeneic bone marrow transplantation (BMT) halts disease progression when stable donor eryth | Full Paper | |
2015 | Krishnamurti, L;Sullivan, K;Kamani, N;Waller, E;Abraham, A;Campigotto, F;Zhang, W;Smith, S;Hassell, K;Decastro, L;Wu, C;Neuberg, D;Walters, M; | Results of a Multicenter Pilot Investigation of Bone Marrow Transplantation in Adults with Sickle Cell Disease (STRIDE) | Blood | Background: Hematopoietic cell transplantation (HCT) for sickle cell disease (SCD) is potentially curative, yet applied sparingly in adults due in part to limited donor availability and to concerns about safety. We conducted a multicenter pilot clini | Full Paper | |
2015 | Cappelli, B;Bernaudin, F;Ruggeri, A;Labopin, M;Volt, F;Simoes, B;Ferster, A;Dupont, S;de la Fuente, J;Dalle, J;Zecca, M;Robin, M;Walters, M;Dhedin, N;Michel, G;Lutz, P;Neven, B;Bertrand, Y;Vannier, J;Ayas, M;Matthes, S;Elayoubi, H;Devergie, A;Kenzey, C;Locatelli, F;Peters, C;Rocha, V;Eapen, M;Gluckman, E; | Hematopoietic Stem Cell Transplantation from HLA Identical Sibling Forsickle Cell Disease an International Survey on Behalf of Eurocord-Monacord, EBMT Paediatric Disease Working Party and CIBMTR | Blood | Introduction: Hematopoietic stem cell transplantation (HSCT) is, to date, the only curative therapy for sickle cell disease (SCD). However, HSCT is offered to relatively few patients with SCD for a number of reasons including lack of a | Full Paper | |
2015 | Kanter, J;Walters, M;Hsieh, M;Thompson, A;Krishnamurti, L;Kwiatkowski, J;Kamble, R;von Kalle, C;Kuypers, F;Cavazzana, M;Leboulch, P;Sandler, L;Soni, S;Tisdale, J; | Initial Results from Study Hgb-206: A Phase 1 Study Evaluating Gene Therapy By Transplantation of Autologous CD34+ Stem Cells Transduced Ex Vivo with the Lentiglobin BB305 Lentiviral Vector in Subjects with Severe Sickle Cell Disease | Blood | Background: Hematopoietic stem cell (HSC) gene transfer has the potential to reduce or eliminate the symptoms of severe sickle cell disease (SCD). Previously reported early results in a single subject with severe SCD from the ongoing study HGB-205 su | Full Paper | |
2015 | Walters, M;Rasko, J;Hongeng, S;Kwiatkowski, J;Schiller, G;Kletzel, M;Ho, P;Vichinsky, E;von Kalle, C;Cavazzana, M;Leboulch, P;Petrusich, A;Soni, S;Thompson, A; | Update of Results from the Northstar Study (HGB-204): A Phase 1/2 Study of Gene Therapy for Beta-Thalassemia Major Via Transplantation of Autologous Hematopoietic Stem Cells Transduced Ex-Vivo with a Lentiviral Beta AT87Q-Globin Vector (LentiGlobin BB305 Drug Product) | Blood | Background: Hematopoietic stem cell (HSC) gene transfer has the potential to induce globin production and mitigate or eliminate blood transfusions in patients with β-thalassemia major. Previously reported early results in subjects with β-thalassemia | Full Paper | |
2015 | Fujishiro, A;Miura, Y;Iwasa, M;Fujii, S;Tamura, A;Sato, A;Yokota, A;Sugino, N;Hirai, H;Ando, A;Ichinohe, T;Maekawa, T; | Vitamin K2 Supports Hematopoiesis through Acting on Bone Marrow Mesenchymal Stromal/Stem Cells | Blood | [Methods] Normal bone marrow (BM) samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA). BM-CD34 + cells were isolated from BM-mononuclear cells Save;[Background] Myelodysplastic syndrome is an intractable disorder cha | Full Paper | |
2015 | Kikushige, Y;Miyamoto, T;Yuda, J;Jabbarzadeh-Tabrizi, S;Shima, T;Takayanagi, S;Niiro, H;Yurino, A;Miyawaki, K;Takenaka, K;Iwasaki, H;Akashi, K; | A TIM-3/Gal-9 Autocrine Stimulatory Loop Drives Self-Renewal of Human Myeloid Leukemia Stem Cells and Leukemic Progression | Cell stem cell | 26279267 | summarizes the primary AML patients’ characteristics analyzed in this study. Human adult BM and PB samples were obtained from healthy donors or purchased from AllCells. CB cells were obtained from full-term deliveries (provided by the Kyushu Block Re | Full Paper |
2015 | Jeong, JY;Levine, MS;Abayasekara, N;Berliner, N;Laubach, J;Vanasse, GJ; | The non-peptide thrombopoietin receptor agonist eltrombopag stimulates megakaryopoiesis in bone marrow cells from patients with relapsed multiple myeloma | Journal of hematology & oncology | 25886818 | were also obtained from commercial sources (AllCells, Emeryville, CA and Proteogenix, from Lonza (Basel, Switzerland) and AllCells, respectively. Normal human platelets were Save | Full Paper |
2015 | Goldstein, RL;Yang, SN;Taldone, T;Chang, B;Gerecitano, J;Elenitoba-Johnson, K;Shaknovich, R;Tam, W;Leonard, JP;Chiosis, G;Cerchietti, L;Melnick, A; | Pharmacoproteomics identifies combinatorial therapy targets for diffuse large B cell lymphoma | The Journal of clinical investigation | 26529251 | HEPES, and penicillin/streptomycin. CD19+ cells were purchased from AllCells. PU-H71 was synthesized as previously reported (5). Ibrutinib was provided by Pharmacyclics. R406, BKM120, and sotrastaurin were purchased from Selleck Chemicals. MI-2 was p | Full Paper |
2015 | Ma, SD;Xu, X;Plowshay, J;Ranheim, EA;Burlingham, WJ;Jensen, JL;Asimakopoulos, F;Tang, W;Gulley, ML;Cesarman, E;Gumperz, JE;Kenney, SC; | LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesis | The Journal of clinical investigation | 25485679 | Alternatively, commercially purchased CD34-depleted human cord blood mononuclear cells (AllCells LLC, catalog no. CB117) were mock-infected or infected with WT EBV or LMP1-KO EBV in vitro for 1.5 hours, after which 12 × 106-25 × 106 cells were inject | Full Paper |
2015 | Chichili, GR;Huang, L;Li, H;Burke, S;He, L;Tang, Q;Jin, L;Gorlatov, S;Ciccarone, V;Chen, F;Koenig, S;Shannon, M;Alderson, R;Moore, PA;Johnson, S;Bonvini, E; | A CD3xCD123 bispecific DART for redirecting host T cells to myelogenous leukemia: preclinical activity and safety in nonhuman primates | Science translational medicine | 26019218 | Cryopreserved AML patient primary PBMCs and bone marrow mononuclear cells (BMMNCs) were purchased from AllCells, LLC, which obtains biological specimens from a donor Save | Full Paper |
2015 | Lee, ST;Muench, MO;Fomin, ME;Xiao, J;Zhou, M;de Smith, A;Martín-Subero, JI;Heath, S;Houseman, EA;Roy, R;Wrensch, M;Wiencke, J;Metayer, C;Wiemels, JL; | Epigenetic remodeling in B-cell acute lymphoblastic leukemia occurs in two tracks and employs embryonic stem cell-like signatures | Nucleic acids research | 25690899 | l, pre-B-II cells for whole genome bisulfite sequencing (WGBS) were purified. Purified normal mature B-cells, T-cells, regulatory T-cells, NK-cells, monocytes and granulocytes were purchased from the Allcells Corp. DNAs were extracted as described ab | Full Paper |
2015 | Jung, N;Dai, B;Gentles, AJ;Majeti, R;Feinberg, AP; | An LSC epigenetic signature is largely mutation independent and implicates the HOXA cluster in AML pathogenesis | Nature communications | 26444494 | n cancer biology using large data sets. Methods Human samples Fresh human bone marrow mononuclear cells (BMMCs) from healthy donors (2 × 108 cells per donor, Catalogue#: ABM006) were purchased from ALLCELLS (Emeryville, CA). A CD34+ cell-enrichment | Full Paper |
2015 | Naka, K;Jomen, Y;Ishihara, K;Kim, J;Ishimoto, T;Bae, EJ;Mohney, RP;Stirdivant, SM;Oshima, H;Oshima, M;Kim, DW;Nakauchi, H;Takihara, Y;Kato, Y;Ooshima, A;Kim, SJ; | Dipeptide species regulate p38MAPK-Smad3 signalling to maintain chronic myelogenous leukaemia stem cells | Nature communications | 26289811 | Viable BM MNCs from three human patients with chronic phase CML were purchased from Allcells (#06-255, #06-620 and #147742, Alameda, CA, USA). We stained these cells with Save | Full Paper |
2015 | Buchner, M;Park, E;Geng, H;Klemm, L;Flach, J;Passegué, E;Schjerven, H;Melnick, A;Paietta, E;Kopanja, D;Raychaudhuri, P;Müschen, M; | Identification of FOXM1 as a therapeutic target in B-cell lineage acute lymphoblastic leukaemia | Nature communications | 25753524 | nslocation of oestrogen receptor fusion proteins, 4-OHT was added at a concentration of 500 nM. Cell sorting For magnetic bead sorting, peripheral blood or BM mononuclear cells were purchased from AllCells LLC and CD19-enrichment was performed by ma | Full Paper |
2015 | Zhang, W;Wray, LS;Rnjak-Kovacina, J;Xu, L;Zou, D;Wang, S;Zhang, M;Dong, J;Li, G;Kaplan, DL;Jiang, X; | Vascularization of hollow channel-modified porous silk scaffolds with endothelial cells for tissue regeneration | Biomaterials | 25934280 | HUVECs were purchased from AllCells biology technology limited company (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth Save | Full Paper |
2015 | Coquery, CM;Loo, WM;Wade, NS;Bederman, AG;Tung, KS;Lewis, JE;Hess, H;Erickson, LD; | BAFF regulates follicular helper t cells and affects their accumulation and interferon-γ production in autoimmunity | Arthritis & rheumatology (Hoboken, N.J.) | 25385309 | Additional blood from SLE patients was purchased from AllCells to increase the range of SLE Disease Activity Index (SLEDAI) scores 24. AllCells used the same protocol and materials Save | Full Paper |
2015 | Stephenson, ST;Brown, LA;Helms, MN;Qu, H;Brown, SD;Brown, MR;Fitzpatrick, AM; | Cysteine oxidation impairs systemic glucocorticoid responsiveness in children with difficult-to-treat asthma | The Journal of allergy and clinical immunology | 25748343 | Cell culture experiments were performed with THP-1 monocytes (ATCC®, Manassas, VA), MM.1S B lymphoblasts (ATCC®) and primary PBMCs from healthy donors (AllCells, Alameda, CA). Reduced and oxidized conditions were created by adding 300 μL of 10 mM cys | Full Paper |
2015 | Karjalainen, K;Jaalouk, DE;Bueso-Ramos, C;Bover, L;Sun, Y;Kuniyasu, A;Driessen, WH;Cardó-Vila, M;Rietz, C;Zurita, AJ;O'Brien, S;Kantarjian, HM;Cortes, JE;Calin, GA;Koivunen, E;Arap, W;Pasqualini, R; | Targeting IL11 Receptor in Leukemia and Lymphoma: A Functional Ligand-Directed Study and Hematopathology Analysis of Patient-Derived Specimens | Clinical cancer research : an official journal of the American Association for Cancer Research | 25779950 | Normal blood and bone marrow samples were commercially obtained (AllCells). Cells were maintained in humidified hypoxia chambers (HeraCell 150; Thermo Electron Corporation) Save | Full Paper |
2015 | Morelli, E;Leone, E;Cantafio, ME;Di Martino, MT;Amodio, N;Biamonte, L;Gullà, A;Foresta, U;Pitari, MR;Botta, C;Rossi, M;Neri, A;Munshi, NC;Anderson, KC;Tagliaferri, P;Tassone, P; | Selective targeting of IRF4 by synthetic microRNA-125b-5p mimics induces anti-multiple myeloma activity in vitro and in vivo | Leukemia | 25987254 | Biosciences, CA, USA); lentiviral particles were produced and transduced as previously described.19 RNA extraction and qRT-PCR. RNA samples of healthy donors BM-derived plasma cells were purchased (AllCells LLC, Alameda, CA, USA). Total RNA extracti | Full Paper |
2015 | Holm, F;Hellqvist, E;Mason, CN;Ali, SA;Delos-Santos, N;Barrett, CL;Chun, HJ;Minden, MD;Moore, RA;Marra, MA;Runza, V;Frazer, KA;Sadarangani, A;Jamieson, CH; | Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewal | Proceedings of the National Academy of Sciences of the United States of America | 26621726 | from patients at the University of California, San Diego and the University of Toronto Health Network according to Institutional Review Board-approved informed consent protocols. Human CD34+ cord blood cells and NPB cells were purchased from AllCells | Full Paper |
2015 | Sundaravel, S;Duggan, R;Bhagat, T;Ebenezer, DL;Liu, H;Yu, Y;Bartenstein, M;Unnikrishnan, M;Karmakar, S;Liu, TC;Torregroza, I;Quenon, T;Anastasi, J;McGraw, KL;Pellagatti, A;Boultwood, J;Yajnik, V;Artz, A;Le Beau, MM;Steidl, U;List, AF;Evans, T;Verma, A;Wickrema, A; | Reduced DOCK4 expression leads to erythroid dysplasia in myelodysplastic syndromes | Proceedings of the National Academy of Sciences of the United States of America | 26578796 | CD34 + HSCs and progenitor cells were purified from G-mobilized peripheral blood of healthy donors purchased from AllCells, Inc. using a CliniMACS (Miltenyi Biotec, Inc.) instrument. Save | Full Paper |
2015 | Yea, K;Zhang, H;Xie, J;Jones, TM;Lin, CW;Francesconi, W;Berton, F;Fallahi, M;Sauer, K;Lerner, RA; | Agonist antibody that induces human malignant cells to kill one another | Proceedings of the National Academy of Sciences of the United States of America | 26487683 | The one patient with M5 differentiated cells had been treated with Ara-C for 4 mo before collection of cells; the other six patients were untreated. Samples of leukemic cells obtained by BM aspiration or from PB were purchased from the AllCells corpo | Full Paper |
2015 | Lovat, F;Fassan, M;Gasparini, P;Rizzotto, L;Cascione, L;Pizzi, M;Vicentini, C;Balatti, V;Palmieri, D;Costinean, S;Croce, CM; | miR-15b/16-2 deletion promotes B-cell malignancies | Proceedings of the National Academy of Sciences of the United States of America | 26324892 | Technologies) according to the manufacturer’s protocol. Real-time PCR experiments were carried out using miR-15b and pri-miR-15b assays for real-time PCR (Applied Biosystems) according to the manufacturer’s protocol. Control human cord blood CD19+ B | Full Paper |
2015 | Mori, Y;Chen, JY;Pluvinage, JV;Seita, J;Weissman, IL; | Prospective isolation of human erythroid lineage-committed progenitors | Proceedings of the National Academy of Sciences of the United States of America | 26195758 | Antibodies, Cell Staining, and Sorting. The sorting procedures for HSCs and myeloid progenitor populations that we previously reported (20) were slightly modified. In brief, bone marrow mononuclear cells, purchased from AllCells, were first stained w | Full Paper |
2015 | Li, Y;Rogoff, HA;Keates, S;Gao, Y;Murikipudi, S;Mikule, K;Leggett, D;Li, W;Pardee, AB;Li, CJ; | Suppression of cancer relapse and metastasis by inhibiting cancer stemness | Proceedings of the National Academy of Sciences of the United States of America | 25605917 | Human CD34 + bone marrow cells (AllCells) were cultured in MethoCult media to form colonies as described by the manufacturer (Stemcell Technologies). BBI608 (2-Acetyl-4H,9H- Save | Full Paper |
2015 | Kuramitsu, M;Okuma, K;Yamochi, T;Sato, T;Sasaki, D;Hasegawa, H;Umeki, K;Kubota, R;Sobata, R;Matsumoto, C;Kaneko, N;Naruse, I;Yamagishi, M;Nakashima, M;Momose, H;Araki, K;Mizukami, T;Mizusawa, S;Okada, Y;Ochiai, M;Utsunomiya, A;Koh, KR;Ogata, M;Nosaka, K;Uchimaru, K;Iwanaga, M;Sagara, Y;Yamano, Y;Satake, M;Okayama, A;Mochizuki, M;Izumo, S;Saito, S;Itabashi, K;Kamihira, S;Yamaguchi, K;Watanabe, T;Hamaguchi, I; | Standardization of Quantitative PCR for Human T-Cell Leukemia Virus Type 1 in Japan: a Collaborative Study | Journal of clinical microbiology | 26292315 | PBMCs were purchased from AllCells Inc. (Alameda, CA, USA). TL-Om1 cells were in RPMI 1640 supplemented with 10% FBS at 37C according to the protocol provided by AllCells Inc. Save | Full Paper |
2015 | Kuramitsu, M;Okuma, K;Yamagishi, M;Yamochi, T;Firouzi, S;Momose, H;Mizukami, T;Takizawa, K;Araki, K;Sugamura, K;Yamaguchi, K;Watanabe, T;Hamaguchi, I; | Identification of TL-Om1, an adult T-cell leukemia (ATL) cell line, as reference material for quantitative PCR for human T-lymphotropic virus 1 | Journal of clinical microbiology | 25502533 | PBMCs were kindly provided by the Japanese Red Cross or purchased from AllCells (Alameda, CA, USA). TL-Om1 cells, an ATL-derived cell line established by Sugamura et al. (17), were maintained in RPMI 1640 (Sigma, St. Louis, MO, USA) containing 10% fe | Full Paper |
2015 | Lancet, JE;Roboz, GJ;Cripe, LD;Michelson, GC;Fox, JA;Leavitt, RD;Chen, T;Hawtin, R;Craig, AR;Ravandi, F;Maris, MB;Stuart, RK;Karp, JE; | A phase 1b/2 study of vosaroxin in combination with cytarabine in patients with relapsed or refractory acute myeloid leukemia | Haematologica | 25381131 | clinical samples, induction of pDNA-PKcs following a 2 h exposure to vosaroxin was examined. PBMC from an AML patient (80% blasts; obtained from AllCells LLC) were treated ex vivo with a dose titration of vosaroxin or vehicle for 2 h. Samples were an | Full Paper |
2015 | Sanyal, M;Morimoto, M;Baradaran-Heravi, A;Choi, K;Kambham, N;Jensen, K;Dutt, S;Dionis-Petersen, KY;Liu, LX;Felix, K;Mayfield, C;Dekel, B;Bokenkamp, A;Fryssira, H;Guillen-Navarro, E;Lama, G;Brugnara, M;Lücke, T;Olney, AH;Hunley, TE;Polat, AI;Yis, U;Bogdanovic, R;Mitrovic, K;Berry, S;Najera, L;Najafian, B;Gentile, M;Nur Semerci, C;Tsimaratos, M;Lewis, DB;Boerkoel, CF; | Lack of IL7Rα expression in T cells is a hallmark of T-cell immunodeficiency in Schimke immuno-osseous dysplasia (SIOD) | Clinical immunology (Orlando, Fla.) | 26499378 | The protocol and informed consent procedures were provided according to the Declaration of Helsinki guidelines. The clinical data for patients were obtained from questionnaires completed by the attending physician as well as from the medical records. | Full Paper |
2015 | Liu, J;Gaj, T;Wallen, MC;Barbas, CF; | Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineering | Molecular therapy. Nucleic acids | 25756962 | T-activator Dynabeads (Dynal/Life Technologies), and 50 U/ml recombinant interleukin-2 (rIL-2; R&D Systems). Bone marrow-derived CD34+ hematopoietic stem/progenitor cells (HSPCs) were obtained from AllCells, LLC at a purity of >97% (Supplementary Fig | Full Paper |
2015 | Kim, SY;Theunissen, JW;Balibalos, J;Liao-Chan, S;Babcock, MC;Wong, T;Cairns, B;Gonzalez, D;van der Horst, EH;Perez, M;Levashova, Z;Chinn, L;D'Alessio, JA;Flory, M;Bermudez, A;Jackson, DY;Ha, E;Monteon, J;Bruhns, MF;Chen, G;Migone, TS; | A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies | Blood cancer journal | 26024286 | Additional frozen AML and CLL patient specimens for flow analysis were from AllCells and the University of California San Diego, respectively. Primary solid tumors and normal Save | Full Paper |
2015 | Crews, LA;Jiang, Q;Zipeto, MA;Lazzari, E;Court, AC;Ali, S;Barrett, CL;Frazer, KA;Jamieson, CH; | An RNA editing fingerprint of cancer stem cell reprogramming | Journal of translational medicine | 25889244 | Peripheral blood or bone marrow samples were processed by Ficoll density centrifugation and viable cells stored in liquid nitrogen. Normal peripheral blood mononuclear cells (MNC) were obtained from AllCells (Alameda, CA). Mononuclear cells from cont | Full Paper |
2015 | Sloan, DD;Lam, CY;Irrinki, A;Liu, L;Tsai, A;Pace, CS;Kaur, J;Murry, JP;Balakrishnan, M;Moore, PA;Johnson, S;Nordstrom, JL;Cihlar, T;Koenig, S; | Targeting HIV Reservoir in Infected CD4 T Cells by Dual-Affinity Re-targeting Molecules (DARTs) that Bind HIV Envelope and Recruit Cytotoxic T Cells | PLoS pathogens | 26539983 | pended with FACS buffer, and analyzed by flow cytometry using a FACSCalibur (BD Biosciences) and FlowJo software (TreeStar). In Vitro HIV-Infected Cell Killing Assay PBMCs from healthy participants (AllCells) were isolated by Ficoll-plaque gradient. | Full Paper |
2015 | Ma, L;Aijima, R;Hoshino, Y;Yamaza, H;Tomoda, E;Tanaka, Y;Sonoda, S;Song, G;Zhao, W;Nonaka, K;Shi, S;Yamaza, T; | Transplantation of mesenchymal stem cells ameliorates secondary osteoporosis through interleukin-17-impaired functions of recipient bone marrow mesenchymal stem cells in MRL/lpr mice | Stem cell research & therapy | 26012584 | University (protocol number: A21-044-1). Human MSC isolation and culture Human MSCs were isolated based on the adherent colony-forming unit fibroblasts (CFU-F) method [20]. Bone marrow cells (BMCs) (AllCells, Berkeley, CA, USA) were seeded at 1×107 | Full Paper |
2015 | Di Lullo, G;Marcatti, M;Heltai, S;Brunetto, E;Tresoldi, C;Bondanza, A;Bonini, C;Ponzoni, M;Tonon, G;Ciceri, F;Bordignon, C;Protti, MP; | Th22 cells increase in poor prognosis multiple myeloma and promote tumor cell growth and survival | Oncoimmunology | 26155400 | had approved the study protocol and written informed consent was obtained from all donors. Blood samples of 15 healthy donors from the Institutional Blood Bank and BM aspirates from 4 healthy donors (AllCells, Life Science Division) were used as cont | Full Paper |
2015 | Alva, A;Friedlander, T;Clark, M;Huebner, T;Daignault, S;Hussain, M;Lee, C;Hafez, K;Hollenbeck, B;Weizer, A;Premasekharan, G;Tran, T;Fu, C;Ionescu-Zanetti, C;Schwartz, M;Fan, A;Paris, P; | Circulating Tumor Cells as Potential Biomarkers in Bladder Cancer | The Journal of urology | 25912492 | candidacy for cisplatin based chemotherapy and 4) candidacy for radical cystectomy with curative intent. Two patients were lost to followup. Of the patients 16 underwent pathological staging at cystectomy. A second cohort of 11 patients with metastas | Full Paper |
2015 | Yang, J;Khan, M;Zhang, L;Ren, X;Guo, J;Feng, Y;Wei, S;Zhang, W; | Antimicrobial surfaces grafted random copolymers with REDV peptide beneficial for endothelialization | Journal of materials chemistry. B | 32264578 | Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells Biomart (Shanghai) and cultured in high glucose DMEM supplemented with 10% FBS in an incubator ( Save | Full Paper |
2015 | Fernandes, S;Chong, JJH;Paige, SL;Iwata, M;Torok-Storb, B;Keller, G;Reinecke, H;Murry, CE; | Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac Repair | Stem cell reports | 26607951 | vol/vol), supplemented with L-glutamine, ascorbic acid, transferrin, monothioglycerol, cyclosporine A (200 nM, Wako), and Y-27632 (10 μM). Fresh bone marrow mononuclear cells were purchased from AllCells LLC (ABM024). After overnight shipment, they | Full Paper |
2015 | McIntosh, BE;Brown, ME;Duffin, BM;Maufort, JP;Vereide, DT;Slukvin, II;Thomson, JA; | Nonirradiated NOD,B6.SCID Il2rγ-/- Kit(W41/W41) (NBSGW) mice support multilineage engraftment of human hematopoietic cells | Stem cell reports | 25601207 | cedures for oligos and allelic determination. The University of Wisconsin Medical School’s Animal Care and Use Committee authorized all experiments. Human Cell Engraftment Frozen human CD34+ CBCs (AllCells) were thawed per manufacturer’s recommendat | Full Paper |
2015 | Tang, W;Song, H;Cai, W;Shen, X; | Real Time Monitoring of Inhibition of Adipogenesis and Angiogenesis by (-)-Epigallocatechin-3-Gallate in 3T3-L1 Adipocytes and Human Umbilical Vein Endothelial Cells | Nutrients | 26516907 | TGTCTTTA-3′ and reverse, 5′-AGCCCTCCACCTCCCTGTAG-3′; β-actin forward, 5′-CCTCTATGCCAACACAGT-3′ and reverse, 5′-AGCCACCAATCCACACAG 3′. 2.8. Co-Culture of HUVEC and 3T3-L1 Cells HUVECs (ALLCELLS, Shanghai, China) were seeded (6000 cells/well) into E-p | Full Paper |
2015 | Griffin, DO;Goff, SP; | HIV-1 Is Restricted prior to Integration of Viral DNA in Primary Cord-Derived Human CD34+ Cells | Journal of virology | 25995256 | CD34 cells were isolated with magnetic beads from cord blood samples obtained from the Long Island Blood Service or obtained preisolated from AllCells (11, 12). CD34 cells were put Save | Full Paper |
2015 | Wu, X;Sereno, AJ;Huang, F;Lewis, SM;Lieu, RL;Weldon, C;Torres, C;Fine, C;Batt, MA;Fitchett, JR;Glasebrook, AL;Kuhlman, B;Demarest, SJ; | Fab-based bispecific antibody formats with robust biophysical properties and biological activity | mAbs | 25774965 | Untreated, purified T cells from a human donor were purchased from ALLCELLS (Cat#PB009-1F). The cells were thawed and washed in 10% FBS (heat inactivated, Corning) plus 50 Save | Full Paper |
2015 | Li, X;Tian, F;Wang, F; | The effects of black soybean (Glycine max var.) on chronic cervical pain of sedentary office workers in a northern Chinese population | Food & function | 26295483 | 5.0 × 105 cells per ml was placed into each well of a 96-well plate (ALLCELLS, Shanghai, China). After 24 h, cells were incubated with 1 Ci per well of [3H]thymidine (67 Ci mmol−1) for five h, and then collected using a Brandell 96-sample harvester ( | Full Paper |
2015 | Mata, MF;Lopes, JP;Ishikawa, M;Alaiti, MA;Cabral, JM;da Silva, CL;Costa, MA; | Scaling up the ex vivo expansion of human circulating CD34(+)progenitor cells with upregulation of angiogenic and anti-inflammatory potential | Cytotherapy | 26475753 | CD34 + cells isolated from mobilized peripheral blood of healthy donors (n = 5) were obtained from AllCells and were cultured for 7 days in 30-mL cell culture bags (VueLife FEP Bag, Save | Full Paper |
2015 | Ma, Y;Reilly, S;Fischer, S;Henshaw, M;Pierce, J;Poudel, A;Reems, J; | Human platelet lysate produced at industrial scale for use as fetal bovine serum replacement in cell manufacturing protocols | Cytotherapy | Tissue Origin: 9 different donors of fresh human bone marrow (hBM) from the posterior iliac crest were obtained from Lonza or AllCells. 6 different donors of fresh human lipoaspirate Save | Full Paper | |
2015 | Fierro, FA;O'Neal, AJ;Beegle, JR;Chávez, MN;Peavy, TR;Isseroff, RR;Egaña, JT; | Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cells | Frontiers in cell and developmental biology | 26579521 | r bone marrow samples of healthy human donors who gave informed consent to the research protocols (approved by the institutional review board of the Technical University of Dresden) or purchased from Allcells (Alameda, CA). Mononuclear cell (MNC) fra | Full Paper |
2015 | Haqqani, AA;Marek, SL;Kumar, J;Davenport, M;Wang, H;Tilton, JC; | Central memory CD4+ T cells are preferential targets of double infection by HIV-1 | Virology journal | 26559763 | Normal donor samples were de-identified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. A 10 year-old male with the Save | Full Paper |
2015 | Huang, H;Saravia, J;You, D;Shaw, AJ;Cormier, SA; | Impaired gamma delta T cell-derived IL-17A and inflammasome activation during early respiratory syncytial virus infection in infants | Immunology and cell biology | 25267484 | tes 2 hr prior to RSV infection. Sample isolation purity and adoptive transfer efficiency were verified by flow cytometry. CBMC/PBMC RIG-I activation Purified CBMCs and PBMCs from individual donors (AllCells.com) were thawed and counted according to | Full Paper |
2015 | Lynch, PJ;Thompson, EE;McGinnis, K;Rovira Gonzalez, YI;Lo Surdo, J;Bauer, SR;Hursh, DA; | Chromatin Changes at the PPAR-γ2 Promoter During Bone Marrow-Derived Multipotent Stromal Cell Culture Correlate With Loss of Gene Activation Potential | Stem cells (Dayton, Ohio) | 25640287 | BM-MSCs from six donors were purchased from AllCells (Alameda, CA, http://www.allcells.com/) and Lonza (Allendale, NJ, http://www.lonza.com/) (Table 1). All BM-MSCs used here were previously expanded, characterized, and cryopreserved for experimentat | Full Paper |
2015 | Münchow, EA;Albuquerque, MT;Zero, B;Kamocki, K;Piva, E;Gregory, RL;Bottino, MC; | Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regeneration | Dental materials : official publication of the Academy of Dental Materials | 26116414 | The guidelines provided by the International Standards Organization/ISO [35] were followed in order to detect toxicity levels of the electrospun membranes [34]. Human dental pulp stem cells (hDPSCs, AllCells LLC., Alameda, CA, USA) obtained from perm | Full Paper |
2015 | Babad, J;Mukherjee, G;Follenzi, A;Ali, R;Roep, BO;Shultz, LD;Santamaria, P;Yang, OO;Goldstein, H;Greiner, DL;DiLorenzo, TP; | Generation of β cell-specific human cytotoxic T cells by lentiviral transduction and their survival in immunodeficient human leucocyte antigen-transgenic mice | Clinical and experimental immunology | 25302633 | Several β cell antigens recognized by T cells in the non-obese diabetic (NOD) mouse model of type 1 diabetes (T1D) are also T cell targets in the human disease. While numerous Save | Full Paper |
2015 | Liu, A;Wang, Y;Ding, Y;Baez, I;Payne, KJ;Borghesi, L; | Cutting Edge: Hematopoietic Stem Cell Expansion and Common Lymphoid Progenitor Depletion Require Hematopoietic-Derived, Cell-Autonomous TLR4 in a Model of Chronic Endotoxin | Journal of immunology (Baltimore, Md. : 1950) | 26276875 | De-identified cord blood cells were obtained with approval from the University of Pittsburgh School of Medicine Institutional Review Board. Purified CD34+ cord blood cells were purchased from AllCells, or cord blood obtained from Loma Linda Universit | Full Paper |
2015 | Katano, I;Takahashi, T;Ito, R;Kamisako, T;Mizusawa, T;Ka, Y;Ogura, T;Suemizu, H;Kawakami, Y;Ito, M; | Predominant development of mature and functional human NK cells in a novel human IL-2-producing transgenic NOG mouse | Journal of immunology (Baltimore, Md. : 1950) | 25712215 | Germany) in combination with biotin-conjugated anti-mouse CD45 mAb (BD Biosciences), all cells, with the exception of human CD56+ NK cells, were magnetically labeled and eliminated using a MACS LD column (Miltenyi Biotec). The purity of the isolated | Full Paper |
2015 | Montezuma-Rusca, JM;Moir, S;Kardava, L;Buckner, CM;Louie, A;Kim, LJ;Santich, BH;Wang, W;Fankuchen, OR;Diaz, G;Daub, JR;Rosenzweig, SD;Chun, TW;Li, Y;Braylan, RC;Calvo, KR;Fauci, AS; | Bone marrow plasma cells are a primary source of serum HIV-1-specific antibodies in chronically infected individuals | Journal of immunology (Baltimore, Md. : 1950) | 25681347 | obtained from posterior iliac crests under conscious sedation and local anesthesia. In addition, cryopreserved bone marrow mononuclear cells (BMMCs) were obtained from commercially available sources (Allcells or Stemcell Technologies) | Full Paper |
2015 | Kang, HS;Choi, SH;Kim, BS;Choi, JY;Park, GB;Kwon, TG;Chun, SY; | Advanced Properties of Urine Derived Stem Cells Compared to Adipose Tissue Derived Stem Cells in Terms of Cell Proliferation, Immune Modulation and Multi Differentiation | Journal of Korean medical science | 26713051 | or separated-(trans-well chamber, Corning, Corning, NY, USA) cultured with both stem cells; each well was composed with 1:100, 1:50, 1:25, or 1:12.5 stem cell to PBMC ratio. PBMCs were acquired from AllCells (Emeryville, CA, USA) and activated with 5 | Full Paper |
2015 | Wu, W;Zhang, X;Lv, H;Liao, Y;Zhang, W;Cheng, H;Deng, Z;Shen, J;Yuan, Q;Zhang, Y;Shen, W; | Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesion | International journal of molecular medicine | 26260137 | cells (HUVECs) were purchased from AllCells (Shanghai, China) and cultured in 0.5% gelatin-coated dishes in endothelial cell growth medium (EGM; AllCells) according to the Save | Full Paper |
2015 | Kamocki, K;Nör, JE;Bottino, MC; | Dental pulp stem cell responses to novel antibiotic-containing scaffolds for regenerative endodontics | International endodontic journal | 25425048 | fetal bovine serum (FBS, Hyclone, Logan, UT, USA) and 1% penicillin- streptomycin (Sigma-Aldrich) in a humidified incubator at 37°C, with 5% CO2, was used to culture hDPSC (catalogue number - DP003F, AllCells LLC, Alameda, CA, USA) obtained from perm | Full Paper |
2015 | Press, T;Viale-Bouroncle, S;Felthaus, O;Gosau, M;Morsczeck, C; | EGR1 supports the osteogenic differentiation of dental stem cells | International endodontic journal | 24749562 | Dental follicle cells (DFCs), also called dental follicle stem cells (DFSCs), were purchased from AllCells© (Alameda, CA, USA). All cells were grown in standard basal medium, Dulbecco′s modified Eagle′s medium (DMEM; Sigma-Aldrich, Munich, Germany) s | Full Paper |
2015 | Lu, Q;Pandya, M;Rufaihah, AJ;Rosa, V;Tong, HJ;Seliktar, D;Toh, WS; | Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel System | Stem cells international | 26124841 | used in this study were obtained commercially from AllCells, LLC. DPSCs were cultured in Dulbecco's modified eagle's medium (DMEM) (low glucose, Biowest, France) supplemented Save | Full Paper |
2015 | Chen, Q;Ye, W;Jian Tan, W;Mei Yong, KS;Liu, M;Qi Tan, S;Loh, E;Te Chang, K;Chye Tan, T;Preiser, PR;Chen, J; | Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in Human | Scientific reports | 26456148 | ercoll. Contaminating red blood cells were lysed with ACK lysis buffer (Life Technologies). Isolation of CD56+ cells from MNCs was performed via cell sorting. Human adult BM MNCs were purchased from AllCells (Alameda, CA). To isolate MNCs from fetal | Full Paper |
2015 | Su, M;Hu, R;Jin, J;Yan, Y;Song, Y;Sullivan, R;Lai, L; | Efficient in vitro generation of functional thymic epithelial progenitors from human embryonic stem cells | Scientific reports | 26044259 | ed (2 Gy), transplanted under the kidney capsule with the solidified reaggregate of the hESC-derived cells, and injected i.v. with human bone marrow CD34+ cells (1 × 105 cells/mouse, obtained from AllCells, LLC, Alameda, CA). Twelve to 24 weeks after | Full Paper |
2015 | Nixon, CC;Schwartz, BH;Dixit, D;Zack, JA;Vatakis, DN; | Cocaine exposure impairs multilineage hematopoiesis of human hematopoietic progenitor cells mediated by the sigma-1 receptor [corrected] | Scientific reports | 25728014 | Mononuclear cells were obtained from the buffy coats of either processed fetal liver or cord blood or adult bone marrow (Allcells, Alameda, CA) following Ficoll-Hypaqe density gradient Save | Full Paper |
2015 | Feitosa, SA;Münchow, EA;Al-Zain, AO;Kamocki, K;Platt, JA;Bottino, MC; | Synthesis and characterization of novel halloysite-incorporated adhesive resins | Journal of dentistry | 26334950 | Next, human dental pulp stem cells (CD73, CD90, CD105, and CD166 positive) obtained from permanent third molars (hDPSCs, catalog number—DP003F, AllCells LLC, Alameda, CA, USA) [32] were cultured in DMEM supplemented with 10% FBS were seeded at the ce | Full Paper |
2015 | Lei, L;Wang, S;Wu, H;Ju, W;Peng, J;Qahtan, AS;Chen, C;Lu, Y;Peng, J;Zhang, X;Nie, H; | Optimization of release pattern of FGF-2 and BMP-2 for osteogenic differentiation of low-population density hMSCs | Journal of biomedical materials research. Part A | 24639043 | Fresh whole bone marrow was acquired from AllCells (Emeryville, CA). 0.5 mL of RosetteSep The hMSCs were isolated according to AllCells' protocol. Cells from passages 4-9 were Save | Full Paper |
2015 | Poleganov, MA;Eminli, S;Beissert, T;Herz, S;Moon, JI;Goldmann, J;Beyer, A;Heck, R;Burkhart, I;Barea Roldan, D;Türeci, Ö;Yi, K;Hamilton, B;Sahin, U; | Efficient Reprogramming of Human Fibroblasts and Blood-Derived Endothelial Progenitor Cells Using Nonmodified RNA for Reprogramming and Immune Evasion | Human gene therapy | 26381596 | Cord blood EPCs were derived from 5E7 frozen cord blood MNCs (AllCells). Both peripheral and cord blood MNCs were washed and cultured on rat collagen type I (BD Bioscience)- Save | Full Paper |
2015 | Suenaga, H;Furukawa, KS;Suzuki, Y;Takato, T;Ushida, T; | Bone regeneration in calvarial defects in a rat model by implantation of human bone marrow-derived mesenchymal stromal cell spheroids | Journal of materials science. Materials in medicine | 26449444 | p). After a recovery period of 8 weeks, bone regeneration at the defect sites was evaluated Isolation and culturing of human MSCs Fresh BM samples of 3-4 anonymous adult donors were obtained from AllCells (Berkeley, CA), and MSCs were isolated, as d | Full Paper |
2015 | Uchiyama, T;Kawabata, H;Miura, Y;Yoshioka, S;Iwasa, M;Yao, H;Sakamoto, S;Fujimoto, M;Haga, H;Kadowaki, N;Maekawa, T;Takaori-Kondo, A; | The role of growth differentiation factor 15 in the pathogenesis of primary myelofibrosis | Cancer medicine | 26276681 | Normal human BM mononuclear cells were separated from BM aspirates (AllCells) using Ficoll-Hypaque-Plus solution (GE Healthcare, Tokyo, Japan). CD34 + HPCs (Hematopoietic Save | Full Paper |
2015 | Wu, H;Fan, F;Liu, Z;Zhang, F;Liu, Y;Wei, Z;Shen, C;Cao, Y;Wang, A;Lu, Y; | The angiogenic responses induced by release of angiogenic proteins from tumor cell-activated platelets are regulated by distinct molecular pathways | IUBMB life | 26283102 | Gibco, Grand Island, NY), 100 U/mL penicillin and 100 μg/mL streptomycin, and grown in a 95% air and 5% CO2 humidified atmosphere at 37°C in Primary HUVECs were purchased from Allcells (Shanghai, China) and cultured in EBM media (Allcells) according | Full Paper |
2015 | Bam, R;Khan, S;Ling, W;Randal, SS;Li, X;Barlogie, B;Edmondson, R;Yaccoby, S; | Primary myeloma interaction and growth in coculture with healthy donor hematopoietic bone marrow | BMC cancer | 26545722 | itute. NBM coculture system Preparation of the NBM system is illustrated in Fig. 1a. Freshly obtained (via overnight shipping) whole BM cells from healthy adult (>40 years old) donors (n = 20, Allcells, Alameda, CA) were lysed with RBC lysis buffer | Full Paper |
2015 | Liao, W;Jordaan, G;Nham, P;Phan, RT;Pelegrini, M;Sharma, S; | Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimens | BMC cancer | 26474785 | In all the CLL specimens, more than 90 % of isolated cells were CD19+ by flow cytometry analysis. Total RNA from isolated B cells (five different normal donors, caucasian males) was purchased from ALLCELLS (Alameda, CA). IGVH mutation (Immunoglobulin | Full Paper |
2015 | Liu, L;Yu, H;Huang, X;Tan, H;Li, S;Luo, Y;Zhang, L;Jiang, S;Jia, H;Xiong, Y;Zhang, R;Huang, Y;Chu, CC;Tian, W; | A novel engineered VEGF blocker with an excellent pharmacokinetic profile and robust anti-tumor activity | BMC cancer | 25881012 | ) in complete HUVEC-adapted medium (Cat#H-004, ALLCELLS) were incubated in 6 cm dishes at 37C, 5% CO 2 for 24 hours, cells were starved for 2 hours and then challenged for 15 Save | Full Paper |
2015 | Priestman, MA;Wang, Q;Jernigan, FE;Chowdhury, R;Schmidt, M;Lawrence, DS; | Multicolor monitoring of the proteasome's catalytic signature | ACS chemical biology | 25347733 | m. All Proteasome inhibitors were purchased from Enzo Life Sciences. HeLa cells were purchased from the tissue culture facility at the University of North Carolina. Normal B-cells were purchased from AllCells. OCI-Ly3 and HBL-1 cells were provided by | Full Paper |
2015 | Yi, Y;Qi, H;Yuan, J;Wang, R;Weng, S;He, J;Dong, C; | Functional characterization of viral tumor necrosis factor receptors encoded by cyprinid herpesvirus 3 (CyHV3) genome | Fish & shellfish immunology | 26052019 | Primary human umbilical vein endothelial cells (HUVECs) were cultured in appropriate media according to the manufacturer's protocol (AllCells, China). The full length of CyHV3 ORF4 Save | Full Paper |
2015 | Doss, JF;Corcoran, DL;Jima, DD;Telen, MJ;Dave, SS;Chi, JT; | A comprehensive joint analysis of the long and short RNA transcriptomes of human erythrocytes | BMC genomics | 26573221 | les as the input list to test for the enrichment of top RBC expressing transcripts against those more specific to D8 samples or the PBMC samples. Cell culture Mobilized Peripheral Blood CD34+ Cells (AllCells) were grown in StemLine II media containi | Full Paper |
2015 | Shi, J;Zhou, J;Zhang, M; | Microcystins induces vascular inflammation in human umbilical vein endothelial cells via activation of NF-B | Mediators of Inflammation | Primary HUVECs were obtained from Allcells (Shanghai, China) and cells in passages 3-5 were used in this study. Cells were grown in an RPMI 1640 medium containing 20% fetal Save | Full Paper | |
2015 | Zhang, Z;Luo, X;Xu, H;Wang, L;Jin, X;Chen, R;Ren, X;Lu, Y;Fu, M;Huang, Y;He, J;Fan, Z; | Bone marrow stromal cell-derived extracellular matrix promotes osteogenesis of adipose-derived stem cells | Cell biology international | 25264269 | BM-MSCs were purchased from ALLCELLS (Emeryville, CA) and expanded in culture medium [α-Minimal Essential Medium (Life Technologies, Grand Island, NY) supplemented with Save | Full Paper |
2015 | Zhang, Q;Wang, LQ;Wong, KY;Li, ZY;Chim, CS; | Infrequent DNA methylation of miR-9-1 and miR-9-3 in multiple myeloma | Journal of clinical pathology | 25855800 | peripheral blood (PB) and three BM normal buffy coat), one CD138-sorted healthy marrow plasma cells (AllCells, California, USA), 10 cell lines (KMS-12-PE, LP-1, MOLP-8, NCI-H929, OPM-2, RPMI-8226, U-266, WL-2, OCI-MY5 and JJN-3), and primary MM marro | Full Paper |
2015 | Wang, DY;Gopinath, S;Lagacé, RE;Norona, W;Hennessy, LK;Short, ML;Mulero, JJ; | Developmental validation of the GlobalFiler(®) Express PCR Amplification Kit: A 6-dye multiplex assay for the direct amplification of reference samples | Forensic science international. Genetics | 26226223 | To demonstrate the sensitivity of the GlobalFiler Express Kit, white blood cells (WBCs) from two individuals were purchased from AllCells, LLC (Emeryville, CA) and quantitated using Save | Full Paper |
2015 | Mahajan, S;Hogan, JK;Shlyakhter, D;Oh, L;Salituro, FG;Farmer, L;Hoock, TC; | VX-509 (decernotinib) is a potent and selective janus kinase 3 inhibitor that attenuates inflammation in animal models of autoimmune disease | The Journal of pharmacology and experimental therapeutics | 25762693 | Sigma-Aldrich (St. Louis, MO); human B cells from AllCells (Emeryville, CA); Roswell Park Memorial Institute medium tissue culture medium, phosphate-buffered saline (PBS), and 10,000 U/ml penicillin-streptomycin from Life Technologies (Grand Island, | Full Paper |
2015 | Shabbir, A;Cox, A;Rodriguez-Menocal, L;Salgado, M;Van Badiavas, E; | Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In Vitro | Stem cells and development | 25867197 | Human MSCs were isolated and expanded from normal donor bone marrow acquired from AllCells LLC (Emeryville, CA, www.allcells.com). Three different donors were used for these experiments. MSCs were isolated by the plastic adherence method as described | Full Paper |
2015 | Limb, JK;Song, D;Jeon, M;Han, SY;Han, G;Jhon, GJ;Bae, YS;Kim, J; | 2-(trimethylammonium)ethyl (R)-3-methoxy-3-oxo-2-stearamidopropyl phosphate promotes megakaryocytic differentiation of myeloid leukaemia cells and primary human CD34⁺ haematopoietic stem cells | Journal of tissue engineering and regenerative medicine | 23166016 | Human bone marrow-derived CD34+ cells were obtained from AllCells (Emeryville, CA, USA). Cells were plated at 8 × 104 cells/well in a 12-well plate and cultured for 16 days in Iscove's modified Dulbecco's medium (IMDM; Invitrogen) with 15% BIT 9500 ( | Full Paper |
2015 | Leelakanok, N;Fischer, CL;Bates, AM;Guthmiller, JM;Johnson, GK;Salem, AK;Brogden, KA;Brogden, NK; | Cytotoxicity of HBD3 for dendritic cells, normal human epidermal keratinocytes, hTERT keratinocytes, and primary oral gingival epithelial keratinocytes in cell culture conditions | Toxicology letters | 26367466 | Human β-defensin 3 (HBD3) is a prominent host defense peptide. In our recent work, we observed that HBD3 modulates pro-inflammatory agonist-induced chemokine and cytokine Save | Full Paper |
2015 | Gupta, MS;Nicoll, SB; | Duration of TGF-β3 Exposure Impacts the Chondrogenic Maturation of Human MSCs in Photocrosslinked Carboxymethylcellulose Hydrogels | Annals of biomedical engineering | 25384834 | Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density centrifugation as previously described.15,28 These primary cells were then cultured using MSC growth medium containing Low Glucose Dulbecco’s Modified Eagle M | Full Paper |
2015 | Marino, MP;Panigaj, M;Ou, W;Manirarora, J;Wei, CH;Reiser, J; | A scalable method to concentrate lentiviral vectors pseudotyped with measles virus glycoproteins | Gene therapy | 25608718 | Human peripheral blood mononuclear cells were obtained from AllCells, LLC (Alameda, CA, USA). T cells were enriched by negative selection using the EasySep Human T Cell Enrichment Cocktail and EasySep D Magnetic Particles (Stem Cell Technologies, Van | Full Paper |
2015 | Shahaduzzaman, MD;Mehta, V;Golden, JE;Rowe, DD;Green, S;Tadinada, R;Foran, EA;Sanberg, PR;Pennypacker, KR;Willing, AE; | Human umbilical cord blood cells induce neuroprotective change in gene expression profile in neurons after ischemia through activation of Akt pathway | Cell transplantation | 25413246 | HUCB Cell Preparation Just prior to the OGD experiment, cryopreserved HUCB mononuclear cells (AllCells, LLC, Alameda, CA, USA) were thawed in a 37ºC water bath and then washed twice in 10 ml PBS (136.9 mM NaCl, 2.7 mM KCl, 10.1 mM Na2HPO4, 1.8 mM KH2 | Full Paper |
2015 | Fukui, T;Mifune, Y;Matsumoto, T;Shoji, T;Kawakami, Y;Kawamoto, A;Ii, M;Akimaru, H;Kuroda, T;Horii, M;Yokoyama, A;Alev, C;Kuroda, R;Kurosaka, M;Asahara, T; | Superior Potential of CD34-Positive Cells Compared to Total Mononuclear Cells for Healing of Nonunion Following Bone Fracture | Cell transplantation | 24800622 | We purchased human GM-PB CD34+ cells obtained from three healthy males (20-year-old African-American; 19-year-old Caucasian; 21-year-old Caucasian) and human GM-PB MNCs obtained from three healthy males (25-year-old African-American; 25-year-old Afri | Full Paper |
2015 | Wada, A;Ito, A;Iitsuka, H;Tsuneyama, K;Miyazono, T;Murakami, J;Shibahara, N;Sakurai, H;Saiki, I;Nakayama, T;Yoshie, O;Koizumi, K;Sugiyama, T; | Role of chemokine CX3CL1 in progression of multiple myeloma via CX3CR1 in bone microenvironments | Oncology reports | 25962684 | GT-3′ and antisense, 5′-CAT GTG GGC CAT GAG GTC CAC CAC-3′. The primers for CX3CR1 were as follows: sense, 5′-TGG CCT TGT CTG ATC TGC TGT TTG-3′ and antisense, 5′-ATG GCT TTG GCT TTC TTG TGG TTC-3′. The RNA from healthy donor plasma cell was purchase | Full Paper |
2015 | Huang, Q;Zhou, C;Chen, X;Dong, B;Chen, S;Zhang, N;Liu, Y;Li, A;Yao, M;Miao, J;Li, Q;Wang, Z; | Prodrug AST-003 Improves the Therapeutic Index of the Multi-Targeted Tyrosine Kinase Inhibitor Sunitinib | PloS one | 26513662 | Dr. Jiang Li at the Sun Yat-Sen University School of Medicine, which were purchased from Type Culture Collection of the Chinese Academy of Sciences in Shanghai, China. HUVEC cells were purchased from Allcells, Inc. The cell lines A549, Caki-1, HT-29, | Full Paper |
2015 | Lanman, RB;Mortimer, SA;Zill, OA;Sebisanovic, D;Lopez, R;Blau, S;Collisson, EA;Divers, SG;Hoon, DS;Kopetz, ES;Lee, J;Nikolinakos, PG;Baca, AM;Kermani, BG;Eltoukhy, H;Talasaz, A; | Analytical and Clinical Validation of a Digital Sequencing Panel for Quantitative, Highly Accurate Evaluation of Cell-Free Circulating Tumor DNA | PloS one | 26474073 | To assess the accuracy of our test, two micrograms of genomic DNA from 20 healthy and young donors (AllCells, Alameda, CA) were sent to an outside reference lab (Ambry Genetics, Save | Full Paper |
2015 | Breese, EH;Buechele, C;Dawson, C;Cleary, ML;Porteus, MH; | Use of Genome Engineering to Create Patient Specific MLL Translocations in Primary Human Hematopoietic Stem and Progenitor Cells | PloS one | 26351841 | When fresh umbilical cord blood was not available, commercially available frozen human CD34+ cells isolated from umbilical cord blood were used (AllCells LLC or StemCell Save | Full Paper |
2015 | Bose, C;Megyesi, JK;Shah, SV;Hiatt, KM;Hall, KA;Karaduta, O;Swaminathan, S; | Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis | PloS one | 26305890 | ked immunosorbent assay (ELISA) by a commercially available kit (GenWay Biotech Inc., San Diego, CA) as instructed by the manufacturer. Cell culture and treatments Commercially available human PBMC (AllCells, Emeryville, CA) were used for in vitro s | Full Paper |
2015 | Ge, HY;Han, ZJ;Tian, P;Sun, WJ;Xue, DX;Bi, Y;Yang, ZH;Liu, P; | VEGFA Expression Is Inhibited by Arsenic Trioxide in HUVECs through the Upregulation of Ets-2 and miRNA-126 | PloS one | 26274316 | , Shanghai, China) were cultured in complete medium (HUVEC-004, supplied by AllCells) in an atmosphere containing 5% CO 2 at 37C. All experiments were performed according to Save | Full Paper |
2015 | Ninomiya, S;Kawano, M;Abe, T;Ishikawa, T;Takahashi, M;Tamura, M;Takahashi, Y;Nashimoto, M; | Potential small guide RNAs for tRNase ZL from human plasma, peripheral blood mononuclear cells, and cultured cell lines | PloS one | 25730316 | myeloma patients (MM1-MM3) were also obtained from AllCells. Written informed consent was obtained from each person by AllCells. In the case that human samples to be analyzed Save | Full Paper |
2015 | Liu, Z;Benard, O;Syeda, MM;Schuster, VL;Chi, Y; | Inhibition of Prostaglandin Transporter (PGT) Promotes Perfusion and Vascularization and Accelerates Wound Healing in Non-Diabetic and Diabetic Rats | PloS one | 26230411 | lonal antibody (LifeSpan BioSciences, Seattle, WA, USA) interacting with rat tissues was used and the dilution was 1: 250. Cell Culture Fresh primary human bone marrow CD34+ cells were obtained from AllCells (Alameda, CA, USA) and cultured according | Full Paper |
2015 | Placencio, VR;Ichimura, A;Miyata, T;DeClerck, YA; | Small Molecule Inhibitors of Plasminogen Activator Inhibitor-1 Elicit Anti-Tumorigenic and Anti-Angiogenic Activity | PloS one | 26207899 | HUVEC (human umbilical vein endothelial cells) were purchased from AllCells and maintained as recommended. HT1080 and HCT116 cells were cultured in DMEM medium Save | Full Paper |
2015 | Fujii, H;Luo, ZJ;Kim, HJ;Newbigging, S;Gassas, A;Keating, A;Egeler, RM; | Humanized Chronic Graft-versus-Host Disease in NOD-SCID il2rγ-/- (NSG) Mice with G-CSF-Mobilized Peripheral Blood Mononuclear Cells following Cyclophosphamide and Total Body Irradiation | PloS one | 26176698 | G-CSF-mobilized human peripheral blood mononuclear cells (G-hPBMCs; 3 donors) and CD34 + cells from G-CSF-mobilized human PB were purchased from ALLCELLS (Emeryvill, CA Save | Full Paper |
2015 | Yan, M;Wen, J;Liang, M;Lu, Y;Kamata, M;Chen, IS; | Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAs | PloS one | 26035832 | edium (Life Technologies, Carlsbad, CA) containing 10% FBS and supplemented with 2mM glutamine, 100 U/ml of penicillin, and 100 μg/ml of streptomycin. Mobilized human CD34+ hematopoietic stem cells (AllCells, Alameda, CA) were cultured in StepSpan SF | Full Paper |
2015 | Patterson, SD;Rossi, JM;Paweletz, KL;Fitzpatrick, VD;Begley, CG;Busse, L;Elliott, S;McCaffery, I; | Functional EpoR pathway utilization is not detected in primary tumor cells isolated from human breast, non-small cell lung, colorectal, and ovarian tumor tissues | PloS one | 25807104 | were adjusted to 106 viable cells/mL prior to growth factor stimulation. For additional cell culture conditions, see S1 Appendix. Erythroid Progenitor-Cell (EPC) Assay Human CD34+ progenitor cells (AllCells Inc., Emeryville, CA) were isolated from b | Full Paper |
2015 | Yu, L;Croze, E;Yamaguchi, KD;Tran, T;Reder, AT;Litvak, V;Volkert, MR; | Induction of a unique isoform of the NCOA7 oxidation resistance gene by interferon β-1b | Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research | 25330068 | Peripheral blood mononuclear cells (PBMCs) derived from healthy individuals were obtained from AllCells. PBMCs (1×106cells/mL) and cultured cells were incubated at 37°C and 5.0% CO2 and grown in Eagle's MEM supplemented with 10% v/v heat-inactivated | Full Paper |
2015 | Liang, X;Potter, J;Kumar, S;Zou, Y;Quintanilla, R;Sridharan, M;Carte, J;Chen, W;Roark, N;Ranganathan, S;Ravinder, N;Chesnut, JD; | Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection | Journal of biotechnology | 26003884 | CD34+ cord blood cells were purchased from AllCells. A549 cells, U-2 OS cells, Neuro-2a (N2A) cells were purchased from ATCC. MEF feeder cells and ROCK inhibitor Y-27632 were Save | Full Paper |
2015 | Kempema, AM;Widen, JC;Hexum, JK;Andrews, TE;Wang, D;Rathe, SK;Meece, FA;Noble, KE;Sachs, Z;Largaespada, DA;Harki, DA; | Synthesis and antileukemic activities of C1-C10-modified parthenolide analogues | Bioorganic & medicinal chemistry | 26088334 | Frozen human mononuclear bone marrow cells were purchased from AllCells (Cat. #ABM011F). These bone marrow cells were from two donors (#5630 [Lot #BM4565] and #4887 [Lot # Save | Full Paper |
2015 | Saraf, S;Araki, H;Petro, B;Park, Y;Taioli, S;Yoshinaga, KG;Koca, E;Rondelli, D;Mahmud, N; | Ex vivo expansion of human mobilized peripheral blood stem cells using epigenetic modifiers | Transfusion | 25363624 | Growth factor-mobilized human MPB or BM cells were obtained from healthy donors either from a commercially available source (AllCells, LLC, Emeryville, CA) or from aliquots of Save | Full Paper |
2015 | Geiger, A;Walker, A;Nissen, E; | Human fibrocyte-derived exosomes accelerate wound healing in genetically diabetic mice | Biochemical and biophysical research communications | 26454169 | Human donor fibrocytes were obtained from leukapheresis packs (AllCells), as previously reported for experiments requiring a high yield of these cells [5], [16], [28]. Viable CD45+[CD3/CD19/CD20/CRTH2]−CD16−CD115−CD11b+CD34+ fibrocytes were enumerate | Full Paper |
2015 | Takahashi, T;Katano, I;Ito, R;Ito, M; | Visualization of the human CD4⁺ T-cell response in humanized HLA-DR4-expressing NOD/Shi-scid/γc(null) (NOG) mice by retrogenic expression of the human TCR gene | Biochemical and biophysical research communications | 25462565 | 2.2. CD34+ hematopoietic stem cells Umbilical CD34+ cells were obtained from AllCells (Alameda, CA). 2.3. DNA constructs BLG-specific CD4+ T-cell clones (HA5.7 and YA4) were kindly provided by Dr. Kondo [14]. The TCRα and β genes were isolated and t | Full Paper |
2015 | Antebi, B;Zhang, Z;Wang, Y;Lu, Z;Chen, XD;Ling, J; | Stromal-cell-derived extracellular matrix promotes the proliferation and retains the osteogenic differentiation capacity of mesenchymal stem cells on three-dimensional scaffolds | Tissue engineering. Part C, Methods | 24965227 | Primary bone-marrow-derived mononuclear cells (MNCs) from human donors 20-30 years of age were purchased from AllCells, LLC (Emeryville, CA). MNCs were cultured on standard Save | Full Paper |
2015 | Akiyama, H;Kobayashi, A;Ichimura, M;Tone, H;Nakatani, M;Inoue, M;Tojo, A;Kagami, H; | Comparison of manual and automated cultures of bone marrow stromal cells for bone tissue engineering | Journal of bioscience and bioengineering | 25907575 | Fresh human bone marrows, obtained from three healthy donors (25- and 28-year-old males and a 38-year-old female), were purchased from AllCells (Emeryville, CA, USA). Each bone marrow was divided into two 20-mL groups, and the manual and automated pr | Full Paper |
2015 | Natu, VP;Dubey, N;Loke, GC;Tan, TS;Ng, WH;Yong, CW;Cao, T;Rosa, V; | Bioactivity, physical and chemical properties of MTA mixed with propylene glycol | Journal of applied oral science : revista FOB | 26398513 | paration of MTA conditioned media The use of human dental pulp stem cells in this study was approved by the Institutional Review Board/NUS (Approval Number: NUS 2094). DPSCs between passages 3 and 6 (Allcells, Alameda, California, USA) were used to e | Full Paper |
2015 | Musa, M;Mohd Ali, K;Kannan, TP;Azlina, A;Omar, NS;Chatterji, A;Mokhtar, KI; | Effects of Perivitelline Fluid Obtained from Horseshoe Crab on The Proliferation and Genotoxicity of Dental Pulp Stem Cells | Cell journal | 26199904 | xicity was evaluated based on the chromosome aberration (CA) and Ames tests. Materials and Methods Study design This was an in vitro experimental study carried out on DPSCs. Cell culture DPSCs from AllCells (USA, cat no. DP004F) were cultured in me | Full Paper |
2015 | Elorza, MT; | Human cytomegalovirus genome replication and maintenance during latent infection | Thesis | Human CD14 (+) cells purchased (Lonza and ReachBio) or isolated from cord blood were maintained in Iscove DMEM (Hyclone) supplemented with 20% heatinactivated FBS (Atlanta Biologicals), 50 ng/mL M-CSF, 50 ng/mL stem cell factor (SCF), 50 ng/mL G-CSF, | Full Paper | |
2015 | Kamocki, K;Nör, JE;Bottino, MC; | Effects of ciprofloxacin-containing antimicrobial scaffolds on dental pulp stem cell viability-In vitro studies | Archives of oral biology | 26042622 | % penicillin-streptomycin (Sigma-Aldrich) placed in a humidified incubator at 37°C, with 5% CO2, was used to culture human dental pulp stem cells (hDPSC, AllCells LLC, Alameda, CA, USA) obtained from permanent third molars. 17,18 The medium was chang | Full Paper |
2015 | Guo, J;Wang, Q;Wai, D;Zhang, QZ;Shi, SH;Le, AD;Shi, ST;Yen, SL; | Visible red and infrared light alters gene expression in human marrow stromal fibroblast cells | Orthodontics & craniofacial research | 25865533 | Commercially available human bone marrow aspirates from two healthy adult donors were purchased from AllCells (Emeryville, CA). Human bone marrow stem cells (HBMSCs) were isolated from the aspirates following published protocols10, 11 and then cultur | Full Paper |
2015 | Thys, RG;Lehman, CE;Pierce, LC;Wang, YH; | Environmental and chemotherapeutic agents induce breakage at genes involved in leukemia-causing gene rearrangements in human hematopoietic stem/progenitor cells | Mutation research | 26163765 | ot been previously connected to leukemogenesis. 2. Materials and methods 2.1. Cells and culture conditions Experiments were performed using primary human CD34+ bone marrow stem and progenitor cells (ALLCELLS), grown in Hematopoietic Growth Medium (L | Full Paper |
2015 | Low, A;Mohd Yusof, H;Reza, F;Abdullah Nurul, A;Sritharan, S;Haania Zain Ali, N;Subhi Azeez, H;Husein, A; | Gypsum-based biomaterials: Evaluation of physical and mechanical properties, cellular effects and its potential as a pulp liner | Dental materials journal | 26235719 | SHED (AllCells, Chicago, IL, USA) were cultivated under sterile conditions in culture flasks containing growth media in a controlled condition at 37°C under a 5% CO2 humidified atmosphere. The culture media consisted of 10% heat-inactivated fetal bov | Full Paper |
2015 | Zhan, H;Ciano, K;Dong, K;Zucker, S; | Targeting glutamine metabolism in myeloproliferative neoplasms | Blood cells, molecules & diseases | 26227854 | Peripheral blood CD34 + cells from healthy controls were purchased from AllCells . Peripheral blood CD34 + cells from healthy controls were purchased from AllCells . Save | Full Paper |
2015 | Ciaffoni, F;Cassella, E;Varricchio, L;Massa, M;Barosi, G;Migliaccio, AR; | Activation of non-canonical TGF-β1 signaling indicates an autoimmune mechanism for bone marrow fibrosis in primary myelofibrosis | Blood cells, molecules & diseases | 25703685 | Signalling profiling was performed on mononuclear cells obtained from BM (n=3) and spleen (n=6) of patients, normal BM (n=3) (ABM008F-BM3366, ABM008F-BM3527 and ABM008F-BM3600, ALLCELLS, Emeryville, CA) and spleen (n=3) from males (30-years old) who | Full Paper |
2015 | Nazarian, AA;Archibeque, IL;Nguyen, YH;Wang, P;Sinclair, AM;Powers, DA; | Characterization of bispecific T-cell Engager (BiTE) antibodies with a high-capacity T-cell dependent cellular cytotoxicity (TDCC) assay | Journal of biomolecular screening | 25477202 | Normal peripheral blood (NPB) was collected via leukapheresis from normal healthy human donors who were nonsmokers and were not taking any allergy medication (AllCells, Save | Full Paper |
2015 | Suknuntha, K;Ishii, Y;Tao, L;Hu, K;McIntosh, BE;Yang, D;Swanson, S;Stewart, R;Wang, JYJ;Thomson, J;Slukvin, I; | Discovery of survival factor for primitive chronic myeloid leukemia cells using induced pluripotent stem cells | Stem cell research | 26561938 | Bone marrow mononuclear cells from CML patients in the chronic phase were purchased commercially (AllCells or Applied StemCells), or obtained from the patients at the University of Save;w specimens from CML patients in the chronic phase, and contro | Full Paper |
2015 | Kanjevac, T;Milovanovic, M;Milosevic-Djordjevic, O;Tesic, Z;Ivanovic, M;Lukic, A; | Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrations | Archives of Biological Sciences | Human SHED purchased from AllCells, LLC (Emeryville, California, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS, 100 I.U./mL penicillin G and 100 μg/mL streptomycin (Sigma-Aldrich | Full Paper | |
2015 | Gonçalves Silva, I;Gibbs, BF;Bardelli, M;Varani, L;Sumbayev, VV; | Differential expression and biochemical activity of the immune receptor Tim-3 in healthy and malignant human myeloid cells | Oncotarget | 26413815 | e highest grade of purity and available commercially unless otherwise stated. Primary human AML cells Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accorda | Full Paper |
2015 | Gibbs, BF;Gonçalves Silva, I;Prokhorov, A;Abooali, M;Yasinska, IM;Casely-Hayford, MA;Berger, SM;Fasler-Kan, E;Sumbayev, VV; | Caffeine affects the biological responses of human hematopoietic cells of myeloid lineage via downregulation of the mTOR pathway and xanthine oxidase activity | Oncotarget | 26384306 | For this purpose we used primary acute myeloid leukaemia cells AML-PB001F purchased from AllCells (Alameda, CA, USA). Cells were exposed to 0.1 μg/ml SCF (these cells express Save | Full Paper |
2015 | Wang, Y;Li, JX;Wang, YQ;Miao, ZH; | Tanshinone I inhibits tumor angiogenesis by reducing Stat3 phosphorylation at Tyr705 and hypoxia-induced HIF-1α accumulation in both endothelial and tumor cells | Oncotarget | 26202747 | ere obtained from the French National Institute of Health and Medical Research (INSERM, FR), and cultured with complete MCDB131 medium (Invitrogen, Carlsbad, CA, USA). HUVEC cells were purchased from AllCells (Shanghai, China). Human breast cancer MC | Full Paper |
2015 | Liu, K;Liu, X;Peng, Z;Sun, H;Zhang, M;Zhang, J;Liu, S;Hao, L;Lu, G;Zheng, K;Gong, X;Wu, D;Wang, F;Shen, L; | Retargeted human avidin-CAR T cells for adoptive immunotherapy of EGFRvIII expressing gliomas and their evaluation via optical imaging | Oncotarget | 26124178 | To isolate peripheral blood mononuclear Cells (PBMC), the red blood cells and platelets were removed with PBMC isolation kits according to the manufacturer's instruction (AllCells, Save | Full Paper |
2015 | Ao, JY;Chai, ZT;Zhang, YY;Zhu, XD;Kong, LQ;Zhang, N;Ye, BG;Cai, H;Gao, DM;Sun, HC; | Robo1 promotes angiogenesis in hepatocellular carcinoma through the Rho family of guanosine triphosphatases' signaling pathway | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine | 26022159 | Robo1 is a member of the Robo immunoglobulin superfamily of proteins, and it plays an important role in angiogenesis and cancer. In this study, we investigate the role of roundabout 1 Save | Full Paper |
2015 | Patel, P;Mahmud, D;Park, Y;Yoshinaga, K;Mahmud, N;Rondelli, D; | Clinical grade isolation of regulatory T cells from G-CSF mobilized peripheral blood improves with initial depletion of monocytes | American journal of blood research | 27069755 | Tregs were purified from G-PBSC (AllCells) obtained from healthy donors. In some cases CD34 + cells were initially isolated on a ClinMACS device (Miltenyi) by positive selection using Save | Full Paper |
2015 | Woodell-May, JE;Tan, ML;King, WJ;Swift, MJ;Welch, ZR;Murphy, MP;McKale, JM; | Characterization of the Cellular Output of a Point-of-Care Device and the Implications for Addressing Critical Limb Ischemia | BioResearch open access | 26634187 | s collected from the iliac crest from healthy financially compensated subjects who qualified for blood donation as per AABB standards. Aspiration was performed by Poietics-Cambrex (Gaithersburg, MD), AllCells (Alameda, CA), and HemaCare (Van Nuys, CA | Full Paper |
2015 | Yao, J;Song, A;Ruan, Z;Zhou, L;Liu, P;Zhu, H;Gong, H;Dong, S;Xu, Y;Jiang, E;Pang, A;Feng, S;Han, M; | [Vascular endothelial injury induced by anti-endothelial cell antibody in allogeneic hematopoietic stem cell transplantation] | Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi | 26134010 | FBS)及BCA定量试剂盒购自美国Thermo Scientific公司;辣 过氧化酶 记的羊抗人IgG购自 美国KPL公司;四甲基联苯胺(TMB)购自美国Tiangen公司;HUVEC及EC完全培养基购自美国Allcells Save | Full Paper |
2015 | Du, C;Weng, X;Hu, W;Lv, Z;Xiao, H;Ding, C;Gyabaah, OA;Xie, H;Zhou, L;Wu, J;Zheng, S; | Hypoxia-inducible MiR-182 promotes angiogenesis by targeting RASA1 in hepatocellular carcinoma | Journal of experimental & clinical cancer research : CR | 26126858 | The HUVECs were obtained from Allcells (Shanghai, China allcells.biomart.cn) and maintained with Serum Free Medium for Endothelial Cells (SFM, Invitrogen) supplemented with 20 Save | Full Paper |
2015 | Kawase, T;Tanaka, T;Okuda, K;Tsuchimochi, M;Oda, M;Hara, T; | Quantitative single-cell motility analysis of platelet-rich plasma-treated endothelial cells in vitro | Cytoskeleton (Hoboken, N.J.) | 25845465 | HUVECs were obtained from AllCells, LLC (Emeryville, CA) and maintained in endothelial cell medium supplemented with growth factors (HuMedia-EB2; KURABO, Osaka, Japan) [Kobayashi et al., 2012]. For the wound healing assay, HUVECs were seeded into dou | Full Paper |
2015 | Fung, C;Mohamad, H;Hashim, S;Htun, A;Ahmad, A; | Proliferative Effect of Malaysian Propolis on Stem Cells from Human Exfoliated Deciduous Teeth: An In vitro Study | British Journal of Pharmaceutical Research | The frozen commercial SHED purchased from AllCells (USA) was retrieved and cultured in alpha minimal essential media (α-MEM) (Gibco, Invitrogen, USA) supplemented with 15% Save | Full Paper | |
2015 | Miura, Y;Ichinohe, T;Maekawa, T; | Human Mesenchymal Stromal/Stem Cell-Mediated Bone Marrow Organization | Japanese Journal of Transfusion and Cell Therapy | In this model, MSCs isolated from bone marrow samples (purchased from AllCells, Emeryville, CA, USA) through the conventional adhesion selection method (BM-MSCs, Fig. A) were Save | Full Paper | |
2015 | Kanazawa, T;Shiba, N;Aizawa, A;Okuno, H;Tamura, K;Tsukada, S;Kumamoto, T;Yamada, S;Kobayashi, Y;Arakawa, H; | Nelarabine Resistance of Childhood T-Cell Lymphoblastic Leukemia/Lymphoma Cells | The Kitakanto Medical Journal | In vitro NEL resistance was seen in half of the testedchildhoodT-ALLcells. ENT-1maypartlyact as a transporter of NEL, but not play a key role in AraC induced NEL resistance. This cell Save | Full Paper | |
2015 | Wu, M;Davis, R;Hatch, A; | Portable microfluidic raman system for rapid, label-free early disease signature detection | Sandia National Laboratories (SNL) | Order blood from Allcells(Alameda, CA), get blood within 4 hours. 2. Morning before blood arrives: 3. Make 50 ml 1x BD lysis buffer (Cat no. 349202), store at 4C. 4. Make 100X stock solutions for stimulation: 1:10 dilution of LPS stock to make 10ug/m | Full Paper | |
2015 | Saka, E; | Image registration and visualization of in situ gene expression images. | University of Louisville | Allcells are built out of similar materials (including an organism's DNA) and function insimilar ways. Prokaryotic cells, such as bacteria and some single celled organisms, havesimple Save | Full Paper | |
2015 | Taha, S;Ling, J;Azmi, N;Kannan, T;Azlina, A;Mokhtar, K; | Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth medium | Brazilian Journal of Oral Sciences | Stem cells from human exfoliated deciduous teeth (SHED) (ALLCells, Alameda, CA, USA) were employed in the current study. SHED was cultured and maintained in T25 cm 2 culture Save | Full Paper | |
2015 | Neises, A;Su, R;Zhang, X; | Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCs | SpringerBriefs in Stem Cells | of water and add 0.9 ml of 5 % EDTA. Adjust the pH to 7.2-7.4. Add water to bring the volume up to 500 ml and filter sterilize through a 0.2-µm filter. Store at 4 °C, but bring to room temperature before use. 4. Alternatively, cord blood CD34+ cells | Full Paper | |
2015 | Doss, J; | The Erythrocyte Transcriptome: Global Characterization and Therapeutic Implication | Thesis | Mobilized Peripheral Blood CD34+ Cells (AllCells) were grown in StemLine II media containing Glutamine (Sigma) with 20% BIT (StemCell Technologies) and 1% Penicillin/Streptomycin (Gibco). On Days 0-7, cells were supplemented with growth factors IL-3 | Full Paper | |
2015 | Ferng, AS; | Cardiac Organogenesis: 3D Bioscaffolds, Bioenergetics and Regeneration | Thesis | Human amniotic stem cells were purchased (AllCells, Alameda, CA) and cultured in Celprogen media (Celprogen, Torrance, CA) under standard cell culture conditions before reseeding on the membrane patches. AmSCs were seeded at 100,000 cells/cm2 per mem | Full Paper | |
2015 | Quarmyne, M; | Protein Tyrosine Phosphatase Receptor Type S (PTPRS) Regulates Hematopoietic Stem Cell Self-Renewal | Thesis | Center according to an approved protocol by the Institutional Review Board of Duke University or the Institutional Biosafety Committee of University of California - Los Angeles. Frozen CB mononuclear cells (MNCs) were obtained from AllCells, LLC., Al | Full Paper | |
2015 | Sanacora, S; | Regulation of IL-8 expression by proteasome in human monocytes and macrophages | Thesis | U937 human promyeloid cells were obtained from American Type Culture Collection (ATCC; Rockville, MD, USA). Peripheral blood mononuclear cells (PBMC) from healthy human volunteers were purchased from AllCells (PB003F; Alameda, CA, USA). The cells wer | Full Paper | |
2015 | Uppal, H;Doudement, E;Mahapatra, K; | Potential Mechanisms for Thrombocytopenia Development with Trastuzumab Emtansine (T-DM1) The Effect of T-DM1 on Platelets and Megakaryocytes | Clin Cancer Res | HSCs (CD133+/CD34+) were purchased from ALLCELLS. Briefly, these HSCs were enriched from bone marrow isolates of healthy donors using positive selection for CD133 and CD34. CD133+/CD34+ cells were first expanded in cytokine-enriched hematopoietic exp | Full Paper | |
2015 | Gupta, MS; | Development of a Carboxymethylcellulose Hydrogel System for Stem Cell-Based Nucleus Pulposus Tissue Engineering | Thesis | Human MSCs were isolated from the bone marrow of a 37 year-old male (AllCells, Emeryville, CA) using enriched density gradient centrifugation. Briefly, a Human MSC Enrichment Cocktail (StemCell Technologies, Vancouver, BC, Canada) (Lee et al. 2005) w | Full Paper | |
2015 | Leon, B; | Discovery, Development, and Applications of Natural Products: Abyssomicin 2, Quinoline Amino Alcohols, and Fluorinated Bile Acids | Thesis | In vitro model of HIV latency. Generation of latent infected cells was performed as previously described.9 Briefly, naive CD4+ T cells were purified by negative selection using PBMCs from healthy HIV negative donors obtained by leukapheresis (AllCell | Full Paper | |
2015 | Kizilyer, A; | The Role of Neurotrophin Signaling in Megakaryocyte Differentiation and Platelet Production | Thesis | CD34+ cells isolated from human bone marrow (BM) or umbilical cord blood were purchased from Allcells (Emeryville, CA, USA), and differentiated according to the previously published protocol [185]. For inducing megakaryocyte progenitor cell different | Full Paper | |
2015 | UCOK, C;KARAAHMETOĞLU, O;UCOK, C; | An analysis of the decision to extract third molar teeth | Thesis | Materials and method: SHED (AllCells, Emeryville, CA; 1 9 105 cells in 2 ml culture medium) were seeded into six-well plates and cultured for 12 h. After the change of fresh medium, LPS (E. coli; 1 lg/ml) or saline (control) was added. Following 3, 1 | Full Paper | |
2015 | OZMERİC KURTULUŞ, N;Selin, A;CANKAL, D; | Treatment of a Patient with Unilateral Cleft Lip and Palate with Free Gingival Greft | Thesis | Materials and method: SHED (AllCells, Emeryville, CA; 1 9 105 cells in 2 ml culture medium) were seeded into six-well plates and cultured for 12 h. After the change of fresh medium, LPS (E. coli; 1 lg/ml) or saline (control) was added. Following 3, 1 | Full Paper | |
2015 | Wang, X;Wei, W;Krzeszinski, JY;Wang, Y;Wan, Y; | A Liver-Bone Endocrine Relay by IGFBP1 Promotes Osteoclastogenesis and Mediates FGF21-Induced Bone Resorption | Cell metabolism | 26456333 | cells were pelleted by centrifugation at 10K rpm for 10min; supernatant was collected as liver-cell-derived conditioned medium (LCM) and used to treat osteoclast differentiation culture. Human PBMN cells (ReachBio) were differentiated into osteoclast | Full Paper |
2015 | Xiang, J;Wu, DC;Chen, Y;Paulson, RF; | In vitro culture of stress erythroid progenitors identifies distinct progenitor populations and analogous human progenitors | Blood | 25608563 | Human bone marrow mononuclear cells were purchased from Reachbio (#033-200) and cultured in the stress erythropoiesis medium described above except that human growth Save | Full Paper |
2015 | Jaramillo, MC;Briehl, MM;Batinic-Haberle, I;Tome, ME; | Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cells | Free radical biology & medicine | 25725417 | The manganese porphyrin, manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin (MnTE-2-PyP 5+), acts as a pro-oxidant in the presence of intracellular H 2 O 2. Mitochondria Save;Normal human peripheral mononuclear blood cells (PMBC) were p | Full Paper |
2015 | Grandi, FC;Rosser, JM;Newkirk, SJ;Yin, J;Jiang, X;Xing, Z;Whitmore, L;Bashir, S;Ivics, Z;Izsvák, Z;Ye, P;Yu, YE;An, W; | Retrotransposition creates sloping shores: a graded influence of hypomethylated CpG islands on flanking CpG sites | Genome research | 25995269 | hnology, Paul Ehrlich Institute, 63225 Langen, Germany 6 These authors contributed equally to this work. 7 Present addresses: Cancer Biology Program, Stanford University, Stanford, CA 94305, USA; 8 ReachBio LLC, Seattle, WA 98107, USA; 9 Departmen | Full Paper |
2015 | Purushothaman, P;Thakker, S;Verma, SC; | Transcriptome analysis of Kaposi's sarcoma-associated herpesvirus during de novo primary infection of human B and endothelial cells | Journal of virology | 25552714 | Human PBMCs (ReachBio, WA) were cultured in RPMI 1640 medium (HyClone) supplemented with 10% FBS, 2 mM l-glutamine, and penicillin-streptomycin (5 U/ml and 5 μg/ml, respectively). Human CD14+ cells were isolated from cord blood received from the Colo | Full Paper |
2015 | Panchanathan, R;Liu, H;Leung, YK;Ho, SM;Choubey, D; | Bisphenol A (BPA) stimulates the interferon signaling and activates the inflammasome activity in myeloid cells | Molecular and cellular endocrinology | 26277401 | Cells lysates were prepared and analyzed for the levels of activated caspase-1 and the mature IL-1β as described (Veeranki et al., 2011). Human purified (CD14+) monocytes from a healthy male donor were purchased from ReachBio (Seattle, WA) and cultur | Full Paper |
2015 | D'Alessandro, JS;Duffner, J;Pradines, J;Capila, I;Garofalo, K;Kaundinya, G;Greenberg, BM;Kantor, D;Ganguly, TC; | Equivalent Gene Expression Profiles between Glatopa™ and Copaxone® | PloS one | 26473741 | tiation of the experiment to minimize bias. All samples were tested on the same day to minimize the introduction of bias resulting from sample processing. First, APC 0.5 mL (naive Balb/C splenocytes, ReachBio LLC, Seattle, WA, USA) cell suspension (1 | Full Paper |
2015 | Tarrant, JM;Dhawan, P;Singh, J;Zabka, TS;Clarke, E;DosSantos, G;Dragovich, PS;Sampath, D;Lin, T;McCray, B;La, N;Nguyen, T;Kauss, A;Dambach, D;Misner, DL;Diaz, D;Uppal, H; | Preclinical models of nicotinamide phosphoribosyltransferase inhibitor-mediated hematotoxicity and mitigation by co-treatment with nicotinic acid | Toxicology mechanisms and methods | 25894564 | Human bone marrow (n = 3) (ReachBio, Seattle, WA) or cynomolgus monkey bone marrow During the conduct of this work, authors were employees of either Genentech or ReachBio. Save | Full Paper |
2015 | Cook, G; | Studies of the efficacy of Ribonucleotide Reductase inhibition in preclinical models of Acute Myeloid Leukemia in combination with standard and novel therapeutics | Thesis | Cells were washed with PBS and resuspended in IMDM supplemented with 20% FBS, and placed in ColonyGel High Cytokine Formulation media (ReachBio). Experiments were performed in triplicate. Colonies were counted on or after day 7. Colonies of 8 or more | Full Paper | |
2015 | Thakker, ST; | Dynamics of KSHV gene expression during de novo infection and the role of LANA in immune modulation | Thesis | Erle S. Robertson, were cultured in endothelial basal medium-2 (Lonza) supplemented with endothelial growth factor supplements (Lonza). Human PBMCs (ReachBio, WA) were cultured in RPMI 1640 medium (Hyclone) supplemented with 10% FBS, 2 mM L-glutamine | Full Paper | |
2014 | Li, S;Tighe, SW;Nicolet, CM;Grove, D;Levy, S;Farmerie, W;Viale, A;Wright, C;Schweitzer, PA;Gao, Y;Kim, D;Boland, J;Hicks, B;Kim, R;Chhangawala, S;Jafari, N;Raghavachari, N;Gandara, J;Garcia-Reyero, N;Hendrickson, C;Roberson, D;Rosenfeld, J;Smith, T;Underwood, JG;Wang, M;Zumbo, P;Baldwin, DA;Grills, GS;Mason, CE; | Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing study | Nat. Biotechnol. | 25150835 | Department of Physiology and Biophysics, Weill Cornell Medical College, New York, New York, USA.++Vermont Cancer Center, University of Vermont, Burlington, Vermont, USA.++Keck School of Medicine, University of Southern California, Los Angeles, Califo | Full Paper |
2014 | Graves, JD;Kordich, JJ;Huang, TH;Piasecki, J;Bush, TL;Sullivan, T;Foltz, IN;Chang, W;Douangpanya, H;Dang, T;O'Neill, JW;Mallari, R;Zhao, X;Branstetter, DG;Rossi, JM;Long, AM;Huang, X;Holland, PM; | Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activity | Cancer Cell | 25043603 | Non-small-cell lung tumor samples were collected under Institutional Review Board approval for each institution, with appropriate informed consent, from surgical resections or after biopsy. Freshly resected human tissue samples were obtained from the | Full Paper |
2014 | Brissova, M;Aamodt, K;Brahmachary, P;Prasad, N;Hong, JY;Dai, C;Mellati, M;Shostak, A;Poffenberger, G;Aramandla, R;Levy, SE;Powers, AC; | Islet microenvironment, modulated by vascular endothelial growth factor-A signaling, promotes β cell regeneration | Cell Metab. | 24561261 | Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA. Electronic address: marcela.brissova@vanderbilt.edu.++Department of Molecular Physiology and Biophysics, Vand | Full Paper |
2014 | Locke, D;Lynch, F;Bernstein, S;Siami-Namini, K;Yarranton, G; | An IHC Screen for EphA3 Positive Myelofibrosis (MF) and Other Myeloproliferative Neoplasms (MPNs) | Blood | Neoplasms (MPNs). Darren Locke, PhD Darren Locke, PhD. 1. QualTek Molecular Laboratories, Newtown,. 1. Search for other works by this author on: This Site. PubMed MPNs. Disclosures. Locke:QualTek Molecular Labs: Employment. Lynch | Full Paper | |
2014 | Fecteau, JF;Corral, LG;Ghia, EM;Gaidarova, S;Futalan, D;Bharati, IS;Cathers, B;Schwaederlé, M;Cui, B;Lopez-Girona, A;Messmer, D;Kipps, TJ; | Lenalidomide inhibits the proliferation of CLL cells via a cereblon/p21(WAF1/Cip1)-dependent mechanism independent of functional p53 | Blood | 24990888 | Blood samples were collected from CLL patients at the University of California San Diego Moores Cancer Center who satisfied diagnostic and immunophenotypic criteria for common B-cell CLL, and who provided written, informed consent, in compliance with | Full Paper |
2014 | Sugihara, K;Kobayashi, Y;Suzuki, A;Tamura, N;Motamedchaboki, K;Huang, CT;Akama, TO;Pecotte, J;Frost, P;Bauer, C;Jimenez, JB;Nakayama, J;Aoki, D;Fukuda, MN; | Development of pro-apoptotic peptides as potential therapy for peritoneal endometriosis | Nat Commun | 25047118 | ... wa (positive) and A431 (negative) cells. A hybridoma clone was subcloned by a limited dilution, establishing the 3B2 clone. Paraffin tissue sections endometriosis patients (_n_=35) were obtained from Folio Biosciences (Columbus, OH). A normal ute | Full Paper |
2014 | Ramesh, R;Kozhaya, L;McKevitt, K;Djuretic, IM;Carlson, TJ;Quintero, MA;McCauley, JL;Abreu, MT;Unutmaz, D;Sundrud, MS; | Pro-inflammatory human Th17 cells selectively express P-glycoprotein and are refractory to glucocorticoids | J. Exp. Med. | 24395888 | ... sue samples. All experiments using human blood and tissue samples were conducted in accordance with IRB protocols approved by committees at Research Blood Components, New York Blood Center, OneBlood, Conversant Bio., and the University of Miami. | Full Paper |
2014 | Van Bortle, K;Nichols, MH;Li, L;Ong, CT;Takenaka, N;Qin, ZS;Corces, VG; | Insulator function and topological domain border strength scale with architectural protein occupancy | Genome Biol. | 24981874 | We are especially thankful to Drs Dale Dorsett, Hugo Bellen, Giovanni Bosco, and Jørgen Johansen for graciously sharing antibodies against Rad21, Barren, CAP-H2, and Chromator. We thank the Drosophila Genomic Resource Center (supported by NIH grant O | Full Paper |
2014 | Steffen, MM;Dearth, SP;Dill, BD;Li, Z;Larsen, KM;Campagna, SR;Wilhelm, SW; | Nutrients drive transcriptional changes that maintain metabolic homeostasis but alter genome architecture in Microcystis | ISME J | 24858783 | ; Scientific, Waltham, MA, USA). Subsequent sample processing, including rRNA subtraction, library prep and sequencing, were performed by the HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA). RNA quality was | Full Paper |
2014 | Picard, M;Zhang, J;Hancock, S;Derbeneva, O;Golhar, R;Golik, P;O'Hearn, S;Levy, S;Potluri, P;Lvova, M;Davila, A;Lin, CS;Perin, JC;Rappaport, EF;Hakonarson, H;Trounce, IA;Procaccio, V;Wallace, DC; | Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional reprogramming | Proc. Natl. Acad. Sci. U.S.A. | 25192935 | Center for Mitochondrial and Epigenomic Medicine, Children's Hospital of Philadelphia and the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104;++School of Biological Sciences, The University of Hong | Full Paper |
2014 | Smith, DC;Eisenberg, PD;Manikhas, G;Chugh, R;Gubens, MA;Stagg, RJ;Kapoun, AM;Xu, L;Dupont, J;Sikic, B; | A phase I dose escalation and expansion study of the anticancer stem cell agent demcizumab (anti-DLL4) in patients with previously treated solid tumors | Clin. Cancer Res. | 25324140 | In addition, control plasma and whole blood samples were sourced and collected (Conversant Bio) using the same methods described above from 8 cancer patients who were not treated with demcizumab. Affymetrix human gene chip U133 Plus 2.0 arrays were u | Full Paper |
2014 | Gee, HY;Ashraf, S;Wan, X;Vega-Warner, V;Esteve-Rudd, J;Lovric, S;Fang, H;Hurd, TW;Sadowski, CE;Allen, SJ;Otto, EA;Korkmaz, E;Washburn, J;Levy, S;Williams, DS;Bakkaloglu, SA;Zolotnitskaya, A;Ozaltin, F;Zhou, W;Hildebrandt, F; | Mutations in EMP2 cause childhood-onset nephrotic syndrome | Am. J. Hum. Genet. | 24814193 | Division of Nephrology, Department of Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA.++Division of Nephrology, Department of Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, US | Full Paper |
2014 | Gee, HY;Otto, EA;Hurd, TW;Ashraf, S;Chaki, M;Cluckey, A;Vega-Warner, V;Saisawat, P;Diaz, KA;Fang, H;Kohl, S;Allen, SJ;Airik, R;Zhou, W;Ramaswami, G;Janssen, S;Fu, C;Innis, JL;Weber, S;Vester, U;Davis, EE;Katsanis, N;Fathy, HM;Jeck, N;Klaus, G;Nayir, A;Rahim, KA;Al Attrach, I;Al Hassoun, I;Ozturk, S;Drozdz, D;Helmchen, U;O'Toole, JF;Attanasio, M;Lewis, RA;Nürnberg, G;Nürnberg, P;Washburn, J;MacDonald, J;Innis, JW;Levy, S;Hildebrandt, F; | Whole-exome resequencing distinguishes cystic kidney diseases from phenocopies in renal ciliopathies | Kidney Int. | 24257694 | Division of Nephrology, Department of Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.++Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, USA.++MRC Human Genetics | Full Paper |
2014 | Rosenfeldt, MT;Bell, LA;Long, JS;O'Prey, J;Nixon, C;Roberts, F;Dufès, C;Ryan, KM; | E2F1 drives chemotherapeutic drug resistance via ABCG2 | Oncogene | 24276245 | Biotechnology (St Louis, MO, USA). The ChIP Assay Kit was from Millipore (Watford, UK) and the human lung cancer tissue microarray was from Folio Biosciences (Columbus, OH, USA). Plasmids. pTRE-E2F1 constructs were generated | Full Paper |
2014 | Wildsmith, KR;Schauer, SP;Smith, AM;Arnott, D;Zhu, Y;Haznedar, J;Kaur, S;Mathews, WR;Honigberg, LA; | Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomics | Mol Neurodegener | 24902845 | ... ap in clinical biomarker development. Methods Source of CSF CSF was purchased from Bioreclamation, LLC (Hicksville, NY) (pooled cynomolgus monkey and pooled human CSF used in discovery experiments), Folio Biosciences (Columbus, OH) (individual l | Full Paper |
2014 | Farber, CR;Reich, A;Barnes, AM;Becerra, P;Rauch, F;Cabral, WA;Bae, A;Quinlan, A;Glorieux, FH;Clemens, TL;Marini, JC; | A novel IFITM5 mutation in severe atypical osteogenesis imperfecta type VI impairs osteoblast production of pigment epithelium-derived factor | J. Bone Miner. Res. | 24519609 | Exome sequencing was performed by the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology (Huntsville, AL, USA). Briefly, gDNA (1 to 2 μg) was fragmented and subjected to exome enrichment using the Nimblegen SeqCap EZ Human Exome Libr | Full Paper |
2014 | Shipitsin, M;Small, C;Choudhury, S;Giladi, E;Friedlander, S;Nardone, J;Hussain, S;Hurley, AD;Ernst, C;Huang, YE;Chang, H;Nifong, TP;Rimm, DL;Dunyak, J;Loda, M;Berman, DM;Blume-Jensen, P; | Identification of proteomic biomarkers predicting prostate cancer aggressiveness and lethality despite biopsy-sampling error | Br. J. Cancer | 25032733 | Acquisition, processing, quality control, and annotation of FFPE prostate cancer tissue blocks. A set of FFPE human prostate cancer tissue blocks with clinical annotations and long-term patient outcome information was acquired from Folio Biosciences | Full Paper |
2014 | Hermetz, KE;Newman, S;Conneely, KN;Martin, CL;Ballif, BC;Shaffer, LG;Cody, JD;Rudd, MK; | Large inverted duplications in the human genome form via a fold-back mechanism | PLoS Genet. | 24497845 | SureSelect capture and Illumina HiSeq sequencing were performed at Hudson Alpha Genomic Services Lab (http://www.hudsonalpha.org/gsl/). After NGS, we aligned 100-bp paired-end reads from fastq files to the GRC37/hg19 reference genome using Burrows-Wh | Full Paper |
2014 | Campbell, VT;Nadesan, P;Ali, SA;Wang, CY;Whetstone, H;Poon, R;Wei, Q;Keilty, J;Proctor, J;Wang, LW;Apte, SS;McGovern, K;Alman, BA;Wunder, JS; | Hedgehog pathway inhibition in chondrosarcoma using the smoothened inhibitor IPI-926 directly inhibits sarcoma cell growth | Mol. Cancer Ther. | 24634412 | Formalin-fixed, paraffin-embedded human chondrosarcoma tissue microarrays (TMA) were purchased from Folio Biosciences for cilia staining. Before staining, all slides were placed in a 60 C oven and baked for 1 hour. Sections were deparaffinized in xyl | Full Paper |
2014 | Folcik, VA;Garofalo, M;Coleman, J;Donegan, JJ;Rabbani, E;Suster, S;Nuovo, A;Magro, CM;Di Leva, G;Nuovo, GJ; | Idiopathic pulmonary fibrosis is strongly associated with productive infection by herpesvirus saimiri | Mod. Pathol. | 24232864 | 1] The Ohio State University (OSU) at Marion, Marion, OH, USA [2] OSU Computer Science and Engineering, Marion, OH, USA [3] OSU Innovation Group for the Study of Complexity in Human, Natural, and Engineered Systems, Marion, OH, USA.++Satellite Labora | Full Paper |
2014 | Lee, CW;Zhan, Q;Lezcano, C;Frank, MH;Huang, J;Larson, AR;Lin, JY;Wan, MT;Lin, PI;Ma, J;Kleffel, S;Schatton, T;Lian, CG;Murphy, GF; | Nestin depletion induces melanoma matrix metalloproteinases and invasion | Lab. Invest. | 25365206 | An additional 153 cases of primary and metastatic melanomas demonstrating clear nodular or infiltrative growth patterns were evaluated for nestin expression in melanoma tissue microarrays (TMA) purchased from Folio Bio (Powell, OH, USA), US Biomax (R | Full Paper |
2014 | Johansson, H;Orjalo, A;Coassin, S;Yin, R; | Abstract 3399: Detection and validation of novel RNA cancer biomarkers by single molecule RNA fluorescence in situ hybridization (smRNA FISH) | Molecular and Cellular Biology | Methods Mol Biol. Ron Cook - Founder, President and CEO Marc Beal - Director of Corporate Development Peter Coassin - Director of Advanced Technology Development Entire Stellaris Team OPKOLab Folio Biosciences #3399 A B A B SK-BR-3 MCF7 T47D | Full Paper | |
2014 | Hornby, PJ;Cooper, PR;Kliwinski, C;Ragwan, E;Mabus, JR;Harman, B;Thompson, S;Kauffman, AL;Yan, Z;Tam, SH;Dorai, H;Powers, GD;Giles-Komar, J; | Human and non-human primate intestinal FcRn expression and immunoglobulin G transcytosis | Pharm. Res. | 24072267 | Paraffin-imbedded 5 μm sections were cut from full-thickness intestine harvested from monkey and human donors, and from human tissue bank (MTB-311 and MTB-316; Qualtek Molecular Labs, Goleta, CA) and dried on glass slides (60°C). Steam heat induced e | Full Paper |
2014 | Jane, EP;Premkumar, DR;Morales, A;Foster, KA;Pollack, IF; | Inhibition of phosphatidylinositol 3-kinase/AKT signaling by NVP-BKM120 promotes ABT-737-induced toxicity in a caspase-dependent manner through mitochondrial dysfunction and DNA damage response in established and primary cultured glioblastoma cells | J. Pharmacol. Exp. Ther. | 24741074 | The human glioma cell lines U87 (PTEN deleted, p53 wild type) and T98G (PTEN mutant, p53 mutant) were purchased from the ATCC (Manassas, VA). LN18 (PTEN wild type, p53 mutant), LN229 (PTEN wild type, p53 mutant), and LNZ308 (PTEN deleted, p53 deleted | Full Paper |
2014 | Hassan, F;Xu, X;Nuovo, G;Killilea, DW;Tyrrell, J;Da Tan, C;Tarran, R;Diaz, P;Jee, J;Knoell, D;Boyaka, PN;Cormet-Boyaka, E; | Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levels | Respir. Res. | 24957904 | ... ell@osumc.edu Boyaka Prosper N 1 boyaka.1@osu.edu Cormet-Boyaka Estelle 1 boyaka.2@osu.edu 1Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA 2Phylogeny Inc., Columbus, OH, USA 3Nutrition a | Full Paper |
2014 | Burger, KL;Learman, BS;Boucherle, AK;Sirintrapun, SJ;Isom, S;Díaz, B;Courtneidge, SA;Seals, DF; | Src-dependent Tks5 phosphorylation regulates invadopodia-associated invasion in prostate cancer cells | Prostate | 24174371 | To conduct an immunohistochemical analysis of Tks5 protein, we purchased a high density prostate tumor microarray (Folio Bioscience, Powell, OH) containing 92, duplicate cases of formalin‐fixed human prostatic adenocarcinoma and 20 cases of normal an | Full Paper |
2014 | Gómez-Díaz, E;Rivero, A;Chandre, F;Corces, VG; | Insights into the epigenomic landscape of the human malaria vector Anopheles gambiae | Front Genet | 25177345 | Total RNA was extracted from fresh mosquito tissues (~25 midguts and ~ 50 salivary glands) using the mirVana™ RNA Isolation Kit (Ambion®) according to the manufacturer's protocol. RNA concentration was quantified using a Qubit® 2.0 Fluorometer, and R | Full Paper |
2014 | Xu, J;Zhao, Z;Zhang, X;Zheng, X;Li, J;Jiang, Y;Kuang, Y;Zhang, Y;Feng, J;Li, C;Yu, J;Li, Q;Zhu, Y;Liu, Y;Xu, P;Sun, X; | Development and evaluation of the first high-throughput SNP array for common carp (Cyprinus carpio) | BMC Genomics | 24762296 | ;... es were collected and DNA was extracted using a DNeasy Blood & Tissue Kit (Qiagen, Shanghai, China). The samples are listed in Table 1. DNA library preparation and sequencing were carried out at the HudsonAlpha Genomic Services Laboratory (Hunts | Full Paper |
2014 | Gruver, AM;Liu, L;Vaillancourt, P;Yan, SC;Cook, JD;Roseberry Baker, JA;Felke, EM;Lacy, ME;Marchal, CC;Szpurka, H;Holzer, TR;Rhoads, EK;Zeng, W;Wortinger, MA;Lu, J;Chow, CK;Denning, IJ;Beuerlein, G;Davies, J;Hanson, JC;Credille, KM;Wijayawardana, SR;Schade, AE; | Immunohistochemical application of a highly sensitive and specific murine monoclonal antibody recognising the extracellular domain of the human hepatocyte growth factor receptor (MET) | Histopathology | 25039923 | both acquired from US Biomax, Rockville, MD, USA), and 67 NSCLC whole‐tissue specimens purchased from Asterand (Detroit, MI, USA), US Biomax, Capital Biosciences, Cybridi (Gaithersburg, MD, USA), Indivumed (Baltimore, MD, USA), Conversant Bio (Huntsv | Full Paper |
2014 | Li, C;Beck, BH;Peatman, E; | Nutritional impacts on gene expression in the surface mucosa of blue catfish (Ictalurus furcatus) | Dev. Comp. Immunol. | 24378224 | ; For each timepoint, equal amounts of RNA from skin and gill were pooled together to generate 3 pooled replicates for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Hun | Full Paper |
2014 | Ehrhardt, N;Bedoya, C;Péterfy, M; | Embryonic viability, lipase deficiency, hypertriglyceridemia and neonatal lethality in a novel LMF1-deficient mouse model | Nutr Metab (Lond) | 25302068 | RT-PCR. p6. CGTGGAGCTTCTTGTGCCT. qPCR. p7. GGATCTGCAGCACTCCAT. qPCR. 36B4-f. CACTGGTCTAGGACCCGAGAAG. qPCR. 36B4-r. GGTGCCTCTGGAGATTTTCG. qPCR. RNA analysis. In situ hybridization was carried out by Phylogeny Inc. as described [11] | Full Paper |
2014 | Choudhary, A;Mambo, E;Sanford, T;Boedigheimer, M;Twomey, B;Califano, J;Hadd, A;Oliner, KS;Beaudenon, S;Latham, GJ;Adai, AT; | Evaluation of an integrated clinical workflow for targeted next-generation sequencing of low-quality tumor DNA using a 51-gene enrichment panel | BMC Med Genomics | 25395014 | The 26 tumor specimens were all colon or rectal adenocarcinomas obtained from Asterand (Detroit, MI, USA). The remaining colon cancer FFPE tissue specimens come from stage II tumors that were acquired from FolioBio (Columbus, OH) | Full Paper |
2014 | Stavropoulos, SW;Sing, RF;Elmasri, F;Silver, MJ;Powell, A;Lynch, FC;Aal, AK;Lansky, AJ;Settlage, RA;Muhs, BE;, ; | The DENALI Trial: an interim analysis of a prospective, multicenter study of the Denali retrievable inferior vena cava filter | J Vasc Interv Radiol | 25066514 | Full Paper | |
2014 | Day, K;Song, J;Absher, D; | Targeted sequencing of large genomic regions with CATCH-Seq | PLoS ONE | 25357200 | HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United States of America.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United States of America.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United Sta | Full Paper |
2014 | Sun, L;Liu, S;Wang, R;Jiang, Y;Zhang, Y;Zhang, J;Bao, L;Kaltenboeck, L;Dunham, R;Waldbieser, G;Liu, Z; | Identification and analysis of genome-wide SNPs provide insight into signatures of selection and domestication in channel catfish (Ictalurus punctatus) | PLoS ONE | 25313648 | ; protocol. Equal amounts of DNA (100 µg) from each individual were pooled for sequencing, one pool for each strain. Sequencing was conducted commercially at HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). Genomic | Full Paper |
2014 | Jiang, Y;Zhang, S;Xu, J;Feng, J;Mahboob, S;Al-Ghanim, KA;Sun, X;Xu, P; | Comparative transcriptome analysis reveals the genetic basis of skin color variation in common carp | PLoS ONE | 25255374 | ;... nufacturer’s instructions. RNA quality was verified using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Equal amounts of high-quality RNA from each tissue were pooled and sent to HudsonAlpha Genomic Services Laboratory (Huntsv | Full Paper |
2014 | Jun, LS;Showalter, AD;Ali, N;Dai, F;Ma, W;Coskun, T;Ficorilli, JV;Wheeler, MB;Michael, MD;Sloop, KW; | A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptor | PLoS ONE | 24695667 | For IHC, tissues and cells were fixed and processed for paraffin embedding using routine procedures as described previously [30]. Paraffin-embedded human pancreas samples were purchased from Folio Biosciences (Columbus, OH). IP-WB and mRNA Expression | Full Paper |
2014 | Luo, Y;Li, C;Landis, AG;Wang, G;Stoeckel, J;Peatman, E; | Transcriptomic profiling of differential responses to drought in two freshwater mussel species, the giant floater Pyganodon grandis and the pondhorn Uniomerus tetralasmus | PLoS ONE | 24586812 | ; construction, Illumina sequencing and expression analysis. RNA-Seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were constructed with | Full Paper |
2014 | Li, SZ;Hu, YY;Zhao, J;Zhao, YB;Sun, JD;Yang, YF;Ji, CC;Liu, ZB;Cao, WD;Qu, Y;Liu, WP;Cheng, G;Fei, Z; | MicroRNA-34a induces apoptosis in the human glioma cell line, A172, through enhanced ROS production and NOX2 expression | Biochem. Biophys. Res. Commun. | 24393844 | 2. Materials and methods. 2.1. Human samples and cell lines. Human glioma cancer tissue microarrays (Lot Number: ARY-HH0118-011-02) containing 60 glioma cancer specimens and 30 normal tissues were purchased from Folio Biosciences (Columbus, OH, USA) | Full Paper |
2014 | Yim, KM;Brewer, MS;Miller, CT;Gillespie, RG; | Comparative transcriptomics of maturity-associated color change in Hawaiian spiders | J. Hered. | 25149253 | Extractions were shipped to the Genomic Services Lab at HudsonAlpha (Huntsville, AL) for library preparation and sequencing. Complementary DNA libraries were prepared from total RNA, and 50bp paired-end Illumina HiSeq 2000 RNA-seq was used to generat | Full Paper |
2014 | Shipitsin, M;Small, C;Giladi, E;Siddiqui, S;Choudhury, S;Hussain, S;Huang, YE;Chang, H;Rimm, DL;Berman, DM;Nifong, TP;Blume-Jensen, P; | Automated quantitative multiplex immunofluorescence in situ imaging identifies phospho-S6 and phospho-PRAS40 as predictive protein biomarkers for prostate cancer lethality | Proteome Sci | 25075204 | In total, we procured and tested 508 unique prostatectomy samples with lethal outcome annotation available (Folio Biosciences, Powell, OH). Of these, 418 passed quality testing and were used for our TMA (Additional file 1: Table S2). Figure 2 Figure | Full Paper |
2014 | Drogemöller, BI;Niehaus, DJ;Chiliza, B;van der Merwe, L;Asmal, L;Malhotra, AK;Wright, GE;Emsley, R;Warnich, L; | Patterns of variation influencing antipsychotic treatment outcomes in South African first-episode schizophrenia patients | Pharmacogenomics | 24444409 | gDNA from the 11 individuals was extracted from venous blood using the QIAamp DNA Blood Maxi Kit (Qiagen, Germany), accord - ing to the manufacturer’s instructions. There - after, the gDNA samples were sent to the HudsonAlpha Genomics Service Labora | Full Paper |
2014 | Zhang, J;Vernes, JM;Ni, J;Nelson, C;Wong, A;Chen, ST;Asundi, A;Vandlen, R;Meng, YG; | Real-time immuno-polymerase chain reaction in a 384-well format: detection of vascular endothelial growth factor and epidermal growth factor-like domain 7 | Anal. Biochem. | 25013989 | CAL-12T cell line was purchased from German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). Human cancer tissues were purchased from ConversantBio (Huntsville, AL) or Avaden BioSciences (Scarsdale, NY) | Full Paper |
2014 | Aycock, KI;Campbell, RL;Manning, KB;Sastry, SP;Shontz, SM;Lynch, FC;Craven, BA; | A computational method for predicting inferior vena cava filter performance on a patient-specific basis | J Biomech Eng | 24805200 | Full Paper | |
2014 | Sarran-Armogan, L; | Prospective isolation of human myeloerythroid progenitors in fetal liver and embryonic stem cell differentiation cultures | Thesis | Einstein Fetal Tissue repository under IRB approved protocol. Human cord blood was obtained from the Obstetric Department at the Weiler Hospital of the Albert Einstein College of Medicine under an IRB approved protocol. Human bone marrow CD34+ cells | Full Paper | |
2014 | Miyake, M;Lawton, A;Goodison, S;Urquidi, V;Rosser, CJ; | Chemokine (C-X-C motif) ligand 1 (CXCL1) protein expression is increased in high-grade prostate cancer | Pathol. Res. Pract. | 24252309 | High-density commercial tissue microarrays (TMA) (ARY-HH0185, Foliobio, Powell, OH) constructed from formalin fixed, paraffin embedded clinical samples comprised the cohort of 110 patients (90 prostate cancer and 20 benign prostates without evidence | Full Paper |
2014 | Pollen, AA;Nowakowski, TJ;Shuga, J;Wang, X;Leyrat, AA;Lui, JH;Li, N;Szpankowski, L;Fowler, B;Chen, P;Ramalingam, N;Sun, G;Thu, M;Norris, M;Lebofsky, R;Toppani, D;Kemp, DW;Wong, M;Clerkson, B;Jones, BN;Wu, S;Knutsson, L;Alvarado, B;Wang, J;Weaver, LS;May, AP;Jones, RC;Unger, MA;Kriegstein, AR;West, JA; | Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex | Nature biotechnology | 25086649 | For systems verification tests of capture efficiency, primary cells were obtained from splenocytes (AllCells, LLC PB003F) and peripheral blood mononuclear cells (AllCells, LLC PB003F) Save | Full Paper |
2014 | Cohen, A; | Regenerative potential of human CD34+ stem cells mobilized and enriched from bone marrow | Thesis | selected from granulocyte colony-stimulating factor (G-CSF) (Amgen; Thousand Oaks, CA, USA) mobilized peripheral blood mononuclear cells (mPBMNCs) obtained from AllCells (Regimen E, Emeryville, CA). Healthy human donors were injected with G-CSF once | Full Paper | |
2014 | Koellmer, M; | Multilineage differentiation of mesenchymal stem cells for cell-based tissue engineering | Thesis | Human bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque TM PLUS solution and RosetteSep® Human Mesenchymal Stem Cell Enrichment Cocktail (Stem Cell Technolog | Full Paper | |
2014 | Lu, M;Xia, L;Li, Y;Wang, X;Hoffman, R; | The orally bioavailable MDM2 antagonist RG7112 and pegylated interferon α 2a target JAK2V617F-positive progenitor and stem cells | Blood | 24869939 | human CD34+ cell selection kit (StemCell Technologies, Vancouver, BC, Canada). Normal human bone marrow (BM) mononuclear cells and CD34+ cells were purchased from AllCells (Emeryville, CA). The experiments for which the CD34+ cells from each of the 3 | Full Paper |
2014 | Shi, J;Rose, EL;Singh, A;Hussain, S;Stagliano, NE;Parry, GC;Panicker, S; | TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutinins | Blood | 24695853 | Human RBCs (hRBCs; blood type O negative; Allcells, Alameda, CA) were washed 3 times with gelatin veronal buffer (GVB; Complement Technology, Tyler, TX) and resuspended at 1 × 109 cells per milliliter in GVB plus 10 mM EDTA. To sensitize hRBCs with C | Full Paper |
2014 | Adamia, S;Bar-Natan, M;Haibe-Kains, B;Pilarski, PM;Bach, C;Pevzner, S;Calimeri, T;Avet-Loiseau, H;Lode, L;Verselis, S;Fox, EA;Galinsky, I;Mathews, S;Dagogo-Jack, I;Wadleigh, M;Steensma, DP;Motyckova, G;Deangelo, DJ;Quackenbush, J;Tenen, DG;Stone, RM;Griffin, JD; | NOTCH2 and FLT3 gene mis-splicings are common events in patients with acute myeloid leukemia (AML): new potential targets in AML | Blood | 24574459 | Our previous studies revealed an increase in alternative splicing of multiple RNAs in cells from patients with acute myeloid leukemia (AML) compared with CD34 + bone marrow cells Save | Full Paper |
2014 | Krow-Lucal, ER;Kim, CC;Burt, TD;McCune, JM; | Distinct functional programming of human fetal and adult monocytes | Blood | 24518760 | Adult bone marrow samples were obtained from healthy donors (AllCells and Lonza Group). Both adult and fetal mononuclear cells were isolated by density centrifugation of a Ficoll- Save | Full Paper |
2014 | Shi, Q;Kuether, EL;Chen, Y;Schroeder, JA;Fahs, SA;Montgomery, RR; | Platelet gene therapy corrects the hemophilic phenotype in immunocompromised hemophilia A mice transplanted with genetically manipulated human cord blood stem cells | Blood | 24269957 | Human cord blood (hCB) CD34+ cells were purchased from AllCells (Emeryville, CA) and transduced with 2bF8LV by using a protocol similar to that described in previous reports.10,15 The details are provided in the supplemental Data (available on the Bl | Full Paper |
2014 | Thompson, A;Rasko, J;Hongeng, S;Kwiatkowski, J;Schiller, G;von Kalle, C;Cavazzana, M;Leboulch, P;Petrusich, A;Soni, S;Walters, M; | Initial Results from the Northstar Study (HGB-204): A Phase 1/2 Study of Gene Therapy for β-Thalassemia Major Via Transplantation of Autologous Hematopoietic Stem Cells Transduced Ex Vivo with a Lentiviral βΑ-T87Q -Globin Vector (LentiGlobin BB305 Drug Product) | Blood | Background: Hematopoietic stem cell (HSC) gene therapy has the potential to induce globin production and mitigate the need for blood transfusions in β-thalassemia major. Promising early results for 2 subjects with β0/βE -thalassemia major in the ongo | Full Paper | |
2014 | Iwasa, M;Miura, Y;Fujishiro, A;Tamura, A;Sato, A;Yokota, A;Nakagawa, Y;Yoshioka, S;Hirai, H;Andoh, A;Ichinohe, T;Maekawa, T; | Effects of Irradiation on the Functional Characteristics of Human Bone Marrow Mesenchymal Stromal/Stem Cells | Blood | BM samples were purchased from AllCells (Emeryville, CA). For the irradiation experiments, BM-MSCs were g-irradiated (Cesium-137) with various doses ranged from 2 to 12 Gy by a Save;Total-body irradiation is frequently used as a conditioning treatm | Full Paper | |
2014 | Sahler, J; | Modulation of Transcription Factors in Platelets and Platelet Microparticles Attenuates Inflammatory Responses | Thesis | Adapted from previous procedures, 94 human CD34+ cord blood cells were purchased from Allcells (Emeryville, CA) and first plated at 2.5x105 cells/mL in IMDM supplemented with 20% BIT 9500, 0.8% Low Density Lipoprotein (L-8292, Sigma), 100ng/mL recomb | Full Paper | |
2014 | Li, S;Garrett-Bakelman, F;Perl, AE;Luger, SM;Zhang, C;To, BL;Lewis, ID;Brown, AL;D'Andrea, RJ;Ross, ME;Levine, R;Carroll, M;Melnick, A;Mason, CE; | Dynamic evolution of clonal epialleles revealed by methclone | Genome biology | 25260792 | samples at two time points (diagnosis and relapse stages), enriched for myeloblast cells, were used in the experiments, and these samples were also compared to five CD34+ normal bone marrow samples (AllCells, Inc.). Institutional review board approva | Full Paper |
2014 | Bartholdy, B;Christopeit, M;Will, B;Mo, Y;Barreyro, L;Yu, Y;Bhagat, TD;Okoye-Okafor, UC;Todorova, TI;Greally, JM;Levine, RL;Melnick, A;Verma, A;Steidl, U; | HSC commitment-associated epigenetic signature is prognostic in acute myeloid leukemia | The Journal of clinical investigation | 24487588 | Acute myeloid leukemia (AML) is characterized by disruption of HSC and progenitor cell differentiation. Frequently, AML is associated with mutations in genes encoding epigenetic Save | Full Paper |
2014 | Schlinker, AC; | In Vitro Platelet Production: Promoting Megakaryocyte Terminal Differentiation, Demonstrating Platelet Functionality, and Developing an Automated Process for Platelet Harvest | Thesis | Figure 4.1. PLT-like particle (PLP) and proPLT production in X-VIVO 10 cultures. (A) Blood PLTs obtained from AllCells (top panels) and PLPs exposed to shear for 3 days after CD61+ selection on day 10 (bottom panels) were stained with anti-CD41 and a | Full Paper | |
2014 | Accomando, WP;Wiencke, JK;Houseman, EA;Nelson, HH;Kelsey, KT; | Quantitative reconstruction of leukocyte subsets using DNA methylation | Genome biology | 24598480 | ummarizes the workflow carried out for all samples, derived from human whole blood, that were utilized in this work. Blood was obtained through an institutional review board-approved donor program at AllCells, LLC (Alameda, CA, USA) and research was | Full Paper |
2014 | Liu, GH;Suzuki, K;Li, M;Qu, J;Montserrat, N;Tarantino, C;Gu, Y;Yi, F;Xu, X;Zhang, W;Ruiz, S;Plongthongkum, N;Zhang, K;Masuda, S;Nivet, E;Tsunekawa, Y;Soligalla, RD;Goebl, A;Aizawa, E;Kim, NY;Kim, J;Dubova, I;Li, Y;Ren, R;Benner, C;Del Sol, A;Bueren, J;Trujillo, JP;Surralles, J;Cappelli, E;Dufour, C;Esteban, CR;Belmonte, JCI; | Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCs | Nature communications | 24999918 | Human BM CD34 + cells from a healthy individual were purchased from Allcells (Alameda, CA). FA patient BM cells were isolated from two FA patients bearing FANCA mutations ( Save | Full Paper |
2014 | Green, MR;Vicente-Dueñas, C;Romero-Camarero, I;Long Liu, C;Dai, B;González-Herrero, I;García-Ramírez, I;Alonso-Escudero, E;Iqbal, J;Chan, WC;Campos-Sanchez, E;Orfao, A;Pintado, B;Flores, T;Blanco, O;Jiménez, R;Martínez-Climent, JA;Criado, FJ;Cenador, MB;Zhao, S;Natkunam, Y;Lossos, IS;Majeti, R;Melnick, A;Cobaleda, C;Alizadeh, AA;Sánchez-García, I; | Transient expression of Bcl6 is sufficient for oncogenic function and induction of mature B-cell lymphoma | Nature communications | 24887457 | Bone marrow from two healthy donors (1 male, 1 female) were obtained purchased from AllCells, LLC. (Emeryville, CA) and sorted on a FACS ARIA II instrument as PI−, Lin−, CD34+, CD38−. Single cells were sorted into Terasaki plates containing alternati | Full Paper |
2014 | Minn, I;Wang, H;Mease, RC;Byun, Y;Yang, X;Wang, J;Leach, SD;Pomper, MG; | A red-shifted fluorescent substrate for aldehyde dehydrogenase | Nature communications | 24759454 | (BD Biosciences, San Jose, CA) with 488 nm blue laser and phycoerythrin (PE) filters. Isolation of ALDHhi human hematopoietic stem cells Frozen human cord blood mononuclear cells were purchased from ALLCELLS (Alamenda, CA). Cells were thawed accordi | Full Paper |
2014 | Verma, R;Su, S;McCrann, DJ;Green, JM;Leu, K;Young, PR;Schatz, PJ;Silva, JC;Stokes, MP;Wojchowski, DM; | RHEX, a novel regulator of human erythroid progenitor cell expansion and erythroblast development | The Journal of experimental medicine | 25092874 | O (and cytokine) challenges were at the concentrations and time intervals indicated. Primary human hematopoietic cells used in RT-PCR analyses included monocytes, neutrophils, T cells, and platelets (AllCells). Murine G1E-ER4 cells (M. Weiss, Childre | Full Paper |
2014 | Roche, ET;Hastings, CL;Lewin, SA;Shvartsman, D;Brudno, Y;Vasilyev, NV;O'Brien, FJ;Walsh, CJ;Duffy, GP;Mooney, DJ; | Comparison of biomaterial delivery vehicles for improving acute retention of stem cells in the infarcted heart | Biomaterials | 24862441 | hMSCs (AllCells, USA) were encapsulated in chitosan/β-GP or alginate gels at a density of 100,000 cells/100μL gel (1 × 106 cells/mL) and 100μL was added to a hanging well cell culture insert with a pore size of 1μm, suitable for insertion into a 48-w | Full Paper |
2014 | Adamia, S;Haibe-Kains, B;Pilarski, PM;Bar-Natan, M;Pevzner, S;Avet-Loiseau, H;Lode, L;Verselis, S;Fox, EA;Burke, J;Galinsky, I;Dagogo-Jack, I;Wadleigh, M;Steensma, DP;Motyckova, G;Deangelo, DJ;Quackenbush, J;Stone, R;Griffin, JD; | A genome-wide aberrant RNA splicing in patients with acute myeloid leukemia identifies novel potential disease markers and therapeutic targets | Clinical cancer research : an official journal of the American Association for Cancer Research | 24284058 | A control group of 12 normal donors of both genders was also included: 8 bone marrow samples of normal donors were obtained from AllCells (California) and 4 peripheral blood Save | Full Paper |
2014 | Wang, X;Moghimi, B;Zolotukhin, I;Morel, LM;Cao, O;Herzog, RW; | Immune tolerance induction to factor IX through B cell gene transfer: TLR9 signaling delineates between tolerogenic and immunogenic B cells | Molecular therapy : the journal of the American Society of Gene Therapy | 24609143 | Primary B cells derived from healthy humans were purchased from Allcells (Alameda, CA). Cell culture reagents were purchased from Invitrogen (Grand Island, NY). Recombinant Save | Full Paper |
2014 | Carbonaro, DA;Zhang, L;Jin, X;Montiel-Equihua, C;Geiger, S;Carmo, M;Cooper, A;Fairbanks, L;Kaufman, ML;Sebire, NJ;Hollis, RP;Blundell, MP;Senadheera, S;Fu, PY;Sahaghian, A;Chan, RY;Wang, X;Cornetta, K;Thrasher, AJ;Kohn, DB;Gaspar, HB; | Preclinical demonstration of lentiviral vector-mediated correction of immunological and metabolic abnormalities in models of adenosine deaminase deficiency | Molecular therapy : the journal of the American Society of Gene Therapy | 24256635 | Normal human adult bone marrow samples (100 ml/donor) were also purchased from AllCells, LLC (Emeryville, CA). Human CD34+ cell isolation was performed as described.51 Briefly, human cord blood or human bone marrow was diluted 1:2 with Dulbecco's pho | Full Paper |
2014 | Huang, X;Shah, S;Wang, J;Ye, Z;Dowey, SN;Tsang, KM;Mendelsohn, LG;Kato, GJ;Kickler, TS;Cheng, L; | Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factors | Molecular therapy : the journal of the American Society of Gene Therapy | 24002691 | Johns Hopkins University Institutional Review Board. Human primary CB MNCs are obtained from anonymous, healthy donors and then isolated in our lab using Ficoll-Paque Plus (P = 1.077) or directly purchased from AllCells, LLC (AllCells, Emeryville, CA | Full Paper |
2014 | Chorzalska, A;Salloum, I;Shafqat, H;Khan, S;Marjon, P;Treaba, D;Schorl, C;Morgan, J;Bryke, CR;Falanga, V;Zhao, TC;Reagan, J;Winer, E;Olszewski, AJ;Al-Homsi, AS;Kouttab, N;Dubielecka, PM; | Low expression of Abelson interactor-1 is linked to acquired drug resistance in Bcr-Abl-induced leukemia | Leukemia | 24699303 | cross-talk may be causative in the acquired drug resistant phenotype of LSCs. Materials and methods Patients and donors Human CD34+ cells were isolated from bone marrow (BM) mononuclear cells (MNC) (AllCells, LLC, Emeryville, CA) or from peripheral | Full Paper |
2014 | Yang, H;Bueso-Ramos, C;DiNardo, C;Estecio, MR;Davanlou, M;Geng, QR;Fang, Z;Nguyen, M;Pierce, S;Wei, Y;Parmar, S;Cortes, J;Kantarjian, H;Garcia-Manero, G; | Expression of PD-L1, PD-L2, PD-1 and CTLA4 in myelodysplastic syndromes is enhanced by treatment with hypomethylating agents | Leukemia | 24270737 | from bone marrow specimens using CD34+ MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s protocol. Bone marrow samples from 4 healthy donors were obtained from Allcells (Emeryville, CA). Peripheral blood (PB) sam | Full Paper |
2014 | Kharabi Masouleh, B;Geng, H;Hurtz, C;Chan, LN;Logan, AC;Chang, MS;Huang, C;Swaminathan, S;Sun, H;Paietta, E;Melnick, AM;Koeffler, P;Müschen, M; | Mechanistic rationale for targeting the unfolded protein response in pre-B acute lymphoblastic leukemia | Proceedings of the National Academy of Sciences of the United States of America | 24821775 | For magnetic bead sorting, peripheral blood or bone marrow mononuclear cells were purchased from AllCells LLC and CD19-enrichment was performed by magnetic bead cell sorting Save | Full Paper |
2014 | Murray, JC;Aldeghaither, D;Wang, S;Nasto, RE;Jablonski, SA;Tang, Y;Weiner, LM; | c-Abl modulates tumor cell sensitivity to antibody-dependent cellular cytotoxicity | Cancer immunology research | 25300860 | Frozen primary peripheral blood mononuclear cells (PBMC) from three individual donors (AllCells) were enriched for NK cells (Human NK Cell Enrichment Kit; STEMCELL Technologies Save | Full Paper |
2014 | Wang, Y;Yi, S;Sun, L;Huang, Y;Zhang, M; | Charge-selective fractions of naturally occurring nanoparticles as bioactive nanocarriers for cancer therapy | Acta biomaterialia | 24952072 | Splenocytes, derived from C57BL/6 mice, were purchased from the Allcells Company (Emeryville, CA). HEPES, 1,9-dimethyl-methylene blue (DMMB), chondroitin sulfate (CS), Sephadex G75, DEAE-cellulose, and phosphate-buffered saline (PBS) were purchased f | Full Paper |
2014 | Fu, Y;Yip, A;Seah, PG;Blasco, F;Shi, PY;Hervé, M; | Modulation of inflammation and pathology during dengue virus infection by p38 MAPK inhibitor SB203580 | Antiviral research | 25131378 | Dengue virus (DENV) infection could lead to dengue fever (DF), dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). The disease outcome is controlled by both viral Save | Full Paper |
2014 | Rampal, R;Alkalin, A;Madzo, J;Vasanthakumar, A;Pronier, E;Patel, J;Li, Y;Ahn, J;Abdel-Wahab, O;Shih, A;Lu, C;Ward, PS;Tsai, JJ;Hricik, T;Tosello, V;Tallman, JE;Zhao, X;Daniels, D;Dai, Q;Ciminio, L;Aifantis, I;He, C;Fuks, F;Tallman, MS;Ferrando, A;Nimer, S;Paietta, E;Thompson, CB;Licht, JD;Mason, CE;Godley, LA;Melnick, A;Figueroa, ME;Levine, RL; | DNA hydroxymethylation profiling reveals that WT1 mutations result in loss of TET2 function in acute myeloid leukemia | Cell reports | 25482556 | Eleven human CD34 + bone marrow samples were provided by the Stem Cell and Xenograft Core Facility of the University of Pennsylvania or purchased from AllCells. These studies Save | Full Paper |
2014 | Li, D;Xie, K;Ding, G;Li, J;Chen, K;Li, H;Qian, J;Jiang, C;Fang, J; | Tumor resistance to anti-VEGF therapy through up-regulation of VEGF-C expression | Cancer letters | 24333721 | Human umbilical vein endothelial cells (HUVECs) were obtained from Allcells (Emeryville, , and amphotericin-B (HUVEC-004; Allcells). All cells were authenticated according to the cell Save | Full Paper |
2014 | Li, X;Tong, LJ;Ding, J;Meng, LH; | Systematic combination screening reveals synergism between rapamycin and sunitinib against human lung cancer | Cancer letters | 24018642 | The human umbilical vein endothelial cells (HUVECs) were purchased from AllCells and maintained in M199 medium supplemented with 20% heat-inactivated FBS, penicillin (100 IU/ Save | Full Paper |
2014 | Yao, WT;Wu, JF;Yu, GY;Wang, R;Wang, K;Li, LH;Chen, P;Jiang, YN;Cheng, H;Lee, HW;Yu, J;Qi, H;Yu, XJ;Wang, P;Chu, YW;Yang, M;Hua, ZC;Ying, HQ;Hoffman, RM;Jeong, LS;Jia, LJ; | Suppression of tumor angiogenesis by targeting the protein neddylation pathway | Cell death & disease | 24525735 | HUVECs were purchased from Allcells Biotech Co. (Shanghai, China) and cultured in a complete endothelial cell medium (Allcells Biotech Co.) at 37C with 5% CO 2 . Save | Full Paper |
2014 | Li, D;Wei, X;Xie, K;Chen, K;Li, J;Fang, J; | A novel decoy receptor fusion protein for FGF-2 potently inhibits tumour growth | British journal of cancer | 24874473 | cells were purchased from AllCells (Emeryville, CA, USA) and cultured in complete HUVEC medium (AllCells) containing 10% FBS and 10% growth factor supplement (AllCells). Caki-1 Save | Full Paper |
2014 | Haselmayer, P;Camps, M;Muzerelle, M;El Bawab, S;Waltzinger, C;Bruns, L;Abla, N;Polokoff, MA;Jond-Necand, C;Gaudet, M;Benoit, A;Bertschy Meier, D;Martin, C;Gretener, D;Lombardi, MS;Grenningloh, R;Ladel, C;Petersen, JS;Gaillard, P;Ji, H; | Characterization of Novel PI3Kδ Inhibitors as Potential Therapeutics for SLE and Lupus Nephritis in Pre-Clinical Studies | Frontiers in immunology | 24904582 | terial smooth muscle cells and keratinocytes (Lonza, Inc., Allendale, NJ, USA) were as previously described. Positively selected primary normal human CD19+ B cells and CD4+ T-cells were obtained from AllCells (Emeryville, CA, USA). PBMC were isolated | Full Paper |
2014 | Fu, J;Tjandra, M;Becker, C;Bednarczyk, D;Capparelli, M;Elling, R;Hanna, I;Fujimoto, R;Furegati, M;Karur, S;Kasprzyk, T;Knapp, M;Leung, K;Li, X;Lu, P;Mergo, W;Miault, C;Ng, S;Parker, D;Peng, Y;Roggo, S;Rivkin, A;Simmons, RL;Wang, M;Wiedmann, B;Weiss, AH;Xiao, L;Xie, L;Xu, W;Yifru, A;Yang, S;Zhou, B;Sweeney, ZK; | Potent nonimmunosuppressive cyclophilin inhibitors with improved pharmaceutical properties and decreased transporter inhibition | Journal of medicinal chemistry | 25310383 | PBMCs of two human donors were obtained on the day of the experiment (AllCells, LLC), pelleted in their original 50 mL conical tubes at 1200 rpm for 10 min, and resuspended in 25 Save | Full Paper |
2014 | Gandhi, AK;Mendy, D;Waldman, M;Chen, G;Rychak, E;Miller, K;Gaidarova, S;Ren, Y;Wang, M;Breider, M;Carmel, G;Mahmoudi, A;Jackson, P;Abbasian, M;Cathers, BE;Schafer, PH;Daniel, TO;Lopez-Girona, A;Thakurta, A;Chopra, R; | Measuring cereblon as a biomarker of response or resistance to lenalidomide and pomalidomide requires use of standardized reagents and understanding of gene complexity | British journal of haematology | 24206017 | here, H929 lenalidomide-resistant cells and DF15R pomalidomide-resistant cells were taken out of culture with compounds for 5-7 d before use. Select primary CD138+ MM cells were purchased from AllCells (Emeryville, CA, USA). Production of recombinant | Full Paper |
2014 | Sully, EK;Malachowa, N;Elmore, BO;Alexander, SM;Femling, JK;Gray, BM;DeLeo, FR;Otto, M;Cheung, AL;Edwards, BS;Sklar, LA;Horswill, AR;Hall, PR;Gresham, HD; | Selective chemical inhibition of agr quorum sensing in Staphylococcus aureus promotes host defense with minimal impact on resistance | PLoS pathogens | 24945495 | Human neutrophils were purchased from AllCells and the source of the neutrophils was Human neutrophils (AllCells) were washed twice in saline to remove EDTA, suspended in RPMI Save | Full Paper |
2014 | Bellayr, IH;Catalano, JG;Lababidi, S;Yang, AX;Lo Surdo, JL;Bauer, SR;Puri, RK; | Gene markers of cellular aging in human multipotent stromal cells in culture | Stem cell research & therapy | 24780490 | Human MSCs from six different donors were purchased from ALLCELLS and Lonza (Table 1). Because these cells were commercially available, no patient consent or approval from the Save | Full Paper |
2014 | Wei, DG;Chiang, V;Fyne, E;Balakrishnan, M;Barnes, T;Graupe, M;Hesselgesser, J;Irrinki, A;Murry, JP;Stepan, G;Stray, KM;Tsai, A;Yu, H;Spindler, J;Kearney, M;Spina, CA;McMahon, D;Lalezari, J;Sloan, D;Mellors, J;Geleziunas, R;Cihlar, T; | Histone deacetylase inhibitor romidepsin induces HIV expression in CD4 T cells from patients on suppressive antiretroviral therapy at concentrations achieved by clinical dosing | PLoS pathogens | 24722454 | ofectamine 2000 (Life Technologies, Grand Island, NY). In vitro model of HIV latency Total peripheral blood mononuclear cells (PBMCs) were obtained from healthy HIV-negative donors by leukapheresis (AllCells, Inc, Emeryville, CA). Naive CD4+ T cells | Full Paper |
2014 | Jajosky, AN;Coad, JE;Vos, JA;Martin, KH;Senft, JR;Wenger, SL;Gibson, LF; | RepSox slows decay of CD34+ acute myeloid leukemia cells and decreases T cell immunoglobulin mucin-3 expression | Stem cells translational medicine | 24855276 | CD34 + cells isolated from the mobilized peripheral blood of healthy donors were purchased from AllCells (Emeryville, CA, http://www.allcells.com). Save | Full Paper |
2014 | Ji, J;Judkowski, VA;Liu, G;Wang, H;Bunying, A;Li, Z;Xu, M;Bender, J;Pinilla, C;Yu, JS; | Identification of novel human leukocyte antigen-A*0201-restricted, cytotoxic T lymphocyte epitopes on CD133 for cancer stem cell immunotherapy | Stem cells translational medicine | 24375541 | bone marrow cells were purchased directly from AllCells Inc. (Alameda, CA, http://www. ) cells (CD133+ and CD133‐ fractions; AllCells) from a healthy donor were included as controls. Save | Full Paper |
2014 | Wang, X;Kimbrel, EA;Ijichi, K;Paul, D;Lazorchak, AS;Chu, J;Kouris, NA;Yavanian, GJ;Lu, SJ;Pachter, JS;Crocker, SJ;Lanza, R;Xu, RH; | Human ESC-derived MSCs outperform bone marrow MSCs in the treatment of an EAE model of multiple sclerosis | Stem cell reports | 25068126 | BM-MSC lines #1, #2 and #3 were derived from frozen BM mononuclear cells (BMMNC) from 3 independent human donors and BM-MSC lines #7 and #8 were derived from fresh whole bone marrow aspirates from 2 independent donors from AllCells, Inc. BM-MSC line | Full Paper |
2014 | Karjalainen, K;Pasqualini, R;Cortes, JE;Kornblau, SM;Lichtiger, B;O'Brien, S;Kantarjian, HM;Sidman, RL;Arap, W;Koivunen, E; | Design, development, and validation of a high-throughput drug-screening assay for targeting of human leukemia | Cancer | 24496871 | negative against blood-transmittable diseases. These samples were stored at 4°C for 24 hours before use. Heparin was used as an anticoagulant. Pretested whole blood and bone marrow samples also were obtained from commercial sources (Innovative Resear | Full Paper |
2014 | Polakowski, N;Terol, M;Hoang, K;Nash, I;Laverdure, S;Gazon, H;Belrose, G;Mesnard, JM;Césaire, R;Péloponèse, JM;Lemasson, I; | HBZ stimulates brain-derived neurotrophic factor/TrkB autocrine/paracrine signaling to promote survival of human T-cell leukemia virus type 1-Infected T cells | Journal of virology | 25210182 | RNA from healthy donor CD4 T cells was purchased from AllCells. cDNA was synthesized Real-time PCR amplification of cDNAs from cell lines and cDNA purchased from AllCells was Save | Full Paper |
2014 | Lucera, MB;Tilton, CA;Mao, H;Dobrowolski, C;Tabler, CO;Haqqani, AA;Karn, J;Tilton, JC; | The histone deacetylase inhibitor vorinostat (SAHA) increases the susceptibility of uninfected CD4+ T cells to HIV by increasing the kinetics and efficiency of postentry viral events | Journal of virology | 25008921 | CD4+ T cells were obtained by leukopheresis from ALLCELLS, LLC. All subjects were 151 Save;CD4 T cells were obtained by leukapheresis from Allcells, LLC. All subjects were healthy control donors negative for hepatitis B virus (HBV), hepatitis C vir | Full Paper |
2014 | Tabler, CO;Lucera, MB;Haqqani, AA;McDonald, DJ;Migueles, SA;Connors, M;Tilton, JC; | CD4+ memory stem cells are infected by HIV-1 in a manner regulated in part by SAMHD1 expression | Journal of virology | 24554663 | Samples from healthy donors were deidentified and were obtained by leukapheresis from AllCells, LLC. HIV-infected patients were recruited from the Clinical Research Center, NIH ( Save | Full Paper |
2014 | Chen, YL;Abdul Ghafar, N;Karuna, R;Fu, Y;Lim, SP;Schul, W;Gu, F;Herve, M;Yokohama, F;Wang, G;Cerny, D;Fink, K;Blasco, F;Shi, PY; | Activation of peripheral blood mononuclear cells by dengue virus infection depotentiates balapiravir | Journal of virology | 24257621 | Antiviral assays. Cryopreserved human PBMCs were purchased from AllCells, LLC (Alameda, California). Written consent from the donors was available for all samples. All experiments involving human PBMCs were approved by the Institutional Review Board | Full Paper |
2014 | Raval, AN;Schmuck, EG;Tefera, G;Leitzke, C;Ark, CV;Hei, D;Centanni, JM;de Silva, R;Koch, J;Chappell, RG;Hematti, P; | Bilateral administration of autologous CD133+ cells in ambulatory patients with refractory critical limb ischemia: lessons learned from a pilot randomized, double-blind, placebo-controlled trial | Cytotherapy | 25239491 | Purity and viability studies were also performed on mobilized leukapheresis products obtained from healthy normal volunteers (AllCells, Almeda, CA, USA). The dose of G-CSF for Save | Full Paper |
2014 | Hulspas, R;Villa-Komaroff, L;Koksal, E;Etienne, K;Rogers, P;Tuttle, M;Korsgren, O;Sharpe, JC;Berglund, D; | Purification of regulatory T cells with the use of a fully enclosed high-speed microfluidic system | Cytotherapy | 25065635 | kit (Stem Cell Technologies Inc, Vancouver, BC, Canada) from a total of 450 million fresh human peripheral blood mononucleated cells obtained from apheresis leuko paks (AllCells Save | Full Paper |
2014 | Dasmahapatra, G;Patel, H;Friedberg, J;Quayle, SN;Jones, SS;Grant, S; | In vitro and in vivo interactions between the HDAC6 inhibitor ricolinostat (ACY1215) and the irreversible proteasome inhibitor carfilzomib in non-Hodgkin lymphoma cells | Molecular cancer therapeutics | 25239935 | Interactions between the HDAC6 inhibitor ricolinostat (ACY1215) and the irreversible proteasome inhibitor carfilzomib were examined in non-Hodgkin lymphoma (NHL) models, Save | Full Paper |
2014 | Feng, JY;Cheng, G;Perry, J;Barauskas, O;Xu, Y;Fenaux, M;Eng, S;Tirunagari, N;Peng, B;Yu, M;Tian, Y;Lee, YJ;Stepan, G;Lagpacan, LL;Jin, D;Hung, M;Ku, KS;Han, B;Kitrinos, K;Perron, M;Birkus, G;Wong, KA;Zhong, W;Kim, CU;Carey, A;Cho, A;Ray, AS; | Inhibition of hepatitis C virus replication by GS-6620, a potent C-nucleoside monophosphate prodrug | Antimicrobial agents and chemotherapy | 24419349 | Human primary bone marrow (BM) light-density cells from three different lots were obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD), and the cytotoxicity studies were Save | Full Paper |
2014 | Yao, H;Miura, Y;Yoshioka, S;Miura, M;Hayashi, Y;Tamura, A;Iwasa, M;Sato, A;Hishita, T;Higashi, Y;Kaneko, H;Ashihara, E;Ichinohe, T;Hirai, H;Maekawa, T; | Parathyroid hormone enhances hematopoietic expansion via upregulation of cadherin-11 in bone marrow mesenchymal stromal cells | Stem cells (Dayton, Ohio) | 24648356 | Parathyroid hormone (PTH) stimulates hematopoiesis in mouse models. The involvement of osteoblasts in this process has been well investigated; however, the effects of PTH on Save | Full Paper |
2014 | Pandya, J;Ortiz, L;Ling, C;Rivers, AE;Aslanidi, G; | Rationally designed capsid and transgene cassette of AAV6 vectors for dendritic cell-based cancer immunotherapy | Immunology and cell biology | 24217810 | Leukapheresis-derived peripheral blood mononuclear cells (AllCells, Alameda, CA, USA) were purified on Ficoll-Paque (GE Healthcare, Piscataway, NJ, USA). Semi-adherent cell fractions were incubated in serum-free X-VIVO medium (Lonza, Allendale, NJ, U | Full Paper |
2014 | Yoshioka, S;Miura, Y;Yao, H;Satake, S;Hayashi, Y;Tamura, A;Hishita, T;Ichinohe, T;Hirai, H;Takaor-Kondo, A;Maekawa, T; | CCAAT/enhancer-binding protein β expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesis | Stem cells (Dayton, Ohio) | 24115241 | The transcription factor CCAAT/enhancer-binding protein β (C/EBPβ) regulates the differentiation of a variety of cell types. Here, the role of C/EBPβ expressed by bone marrow Save | Full Paper |
2014 | Brunckhorst, MK;Xu, Y;Lu, R;Yu, Q; | Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironment | The American journal of pathology | 25043619 | Despite decades of research, the survival rate of ovarian cancer patients is largely unchanged. Current chemotherapeutic drugs are effective only transiently because patients with Save | Full Paper |
2014 | Bowers, PM;Verdino, P;Wang, Z;da Silva Correia, J;Chhoa, M;Macondray, G;Do, M;Neben, TY;Horlick, RA;Stanfield, RL;Wilson, IA;King, DJ; | Nucleotide insertions and deletions complement point mutations to massively expand the diversity created by somatic hypermutation of antibodies | The Journal of biological chemistry | 25320089 | Sequencing and Preparation of PBMC cDNA RNA from peripheral blood lymphocytes (PBMCs) from a total of 68 healthy donors was purchased from two sources as follows: seven donors from AllCells, Inc. (Alameda, CA), and 61 donors from HemaCare Corp. (San | Full Paper |
2014 | Edwards, W;Fung-Leung, WP;Huang, C;Chi, E;Wu, N;Liu, Y;Maher, MP;Bonesteel, R;Connor, J;Fellows, R;Garcia, E;Lee, J;Lu, L;Ngo, K;Scott, B;Zhou, H;Swanson, RV;Wickenden, AD; | Targeting the ion channel Kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-life | The Journal of biological chemistry | 24939846 | Human PBMCs were obtained from AllCells LLC or purified from healthy donor blood by Ficoll-Paque (GE Healthcare) density centrifugation. Purified PBMCs were plated in 96-well Save | Full Paper |
2014 | Stuible, M;Moraitis, A;Fortin, A;Saragosa, S;Kalbakji, A;Filion, M;Tremblay, GB; | Mechanism and function of monoclonal antibodies targeting siglec-15 for therapeutic inhibition of osteoclastic bone resorption | The Journal of biological chemistry | 24446437 | HOPs (CD14 + peripheral blood mononuclear cells) were isolated from normal human peripheral blood mononuclear cells (AllCells, Emeryville, CA) using CD14 microbeads and MS Save | Full Paper |
2014 | Collins, E;Gu, F;Qi, M;Molano, I;Ruiz, P;Sun, L;Gilkeson, GS; | Differential efficacy of human mesenchymal stem cells based on source of origin | Journal of immunology (Baltimore, Md. : 1950) | 25274529 | Human BM MSCs were derived from fresh whole BM of healthy donors (purchased from ALLCELLS) and four lupus patients. Lupus patients consisted of four African Americans (three Save | Full Paper |
2014 | Lan, J;Yang, K;Byrd, D;Hu, N;Amet, T;Shepherd, N;Desai, M;Gao, J;Gupta, S;Sun, Y;Yu, Q; | Provirus activation plus CD59 blockage triggers antibody-dependent complement-mediated lysis of latently HIV-1-infected cells | Journal of immunology (Baltimore, Md. : 1950) | 25149467 | Briefly, leukapheresis products from 3 HIV-1-negative blood donors were purchased from the ALLCells (Emeryville, CA) for isolation of primary CD4+ T cells using the negative selection RosetteSep reagents (StemCell Technologies, Vancouver, BC, Canada) | Full Paper |
2014 | Balan, S;Ollion, V;Colletti, N;Chelbi, R;Montanana-Sanchis, F;Liu, H;Vu Manh, TP;Sanchez, C;Savoret, J;Perrot, I;Doffin, AC;Fossum, E;Bechlian, D;Chabannon, C;Bogen, B;Asselin-Paturel, C;Shaw, M;Soos, T;Caux, C;Valladeau-Guilemond, J;Dalod, M; | Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells | Journal of immunology (Baltimore, Md. : 1950) | 25009205 | CD34 + cells from adult donor bone marrow or mobilized peripheral blood were purchased from properly licensed commercial companies (AllCells and StemCells). Save | Full Paper |
2014 | Kajiwara, Y;Schiff, T;Voloudakis, G;Gama Sosa, MA;Elder, G;Bozdagi, O;Buxbaum, JD; | A critical role for human caspase-4 in endotoxin sensitivity | Journal of immunology (Baltimore, Md. : 1950) | 24879791 | Human monocyte-derived macrophages were purchased from AllCells (PB-MDM-001F; Alameda, CA) and cultured in RPMI 1640 supplemented with 10% FBS/penicillin/streptomycin and 10 ng/ml human M-CSF (R&D Systems). BMDM cultures were prepared according to pr | Full Paper |
2014 | Kohn, LA;Seet, CS;Scholes, J;Codrea, F;Chan, R;Zaidi-Merchant, S;Zhu, Y;De Oliveira, S;Kapoor, N;Shah, A;Abdel-Azim, H;Kohn, DB;Crooks, GM; | Human lymphoid development in the absence of common γ-chain receptor signaling | Journal of immunology (Baltimore, Md. : 1950) | 24771849 | Normal human adult BM was obtained from healthy donors as anonymous waste materials isolated from filters after BM harvest and from commercial sources (AllCells, Alameda, CA). Save | Full Paper |
2014 | Inage, E;Kasakura, K;Yashiro, T;Suzuki, R;Baba, Y;Nakano, N;Hara, M;Tanabe, A;Oboki, K;Matsumoto, K;Saito, H;Niyonsaba, F;Ohtsuka, Y;Ogawa, H;Okumura, K;Shimizu, T;Nishiyama, C; | Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2 | Journal of immunology (Baltimore, Md. : 1950) | 24639354 | Human primary mast cells were generated from peripheral blood CD34+ cells (Frozen Mobilized Peripheral Blood CD34+ cells, mPB015F; ALLCELLS, Emeryville, CA) as progenitors based on the previously established method (13). In three independent experime | Full Paper |
2014 | Fukahori, H;Chida, N;Maeda, M;Tasaki, M;Kawashima, T;Matsuoka, H;Suzuki, K;Ishikawa, T;Tanaka, A;Higashi, Y; | Effect of AS2521780, a novel PKCθ selective inhibitor, on T cell-mediated immunity | European journal of pharmacology | 25445039 | T cell-mediated immunity is central to the pathogenesis of autoimmune diseases, and is a target in the development of alternative therapeutic strategies with reduced adverse effects on Save | Full Paper |
2014 | Kota, DJ;DiCarlo, B;Hetz, RA;Smith, P;Cox, CS;Olson, SD; | Differential MSC activation leads to distinct mononuclear leukocyte binding mechanisms | Scientific reports | 24691433 | For Supplemental Figure 1, we also isolated MSCs from commercially available fresh bone marrow aspirates (AllCells, Emeryville, CA) according to the protocols previously established Save | Full Paper |
2014 | Lee, JS;Kim, YR;Park, JM;Ha, SJ;Kim, YE;Baek, NI;Hong, EK; | Mulberry fruit extract protects pancreatic β-cells against hydrogen peroxide-induced apoptosis via antioxidative activity | Molecules (Basel, Switzerland) | 24972274 | and then 0.7 mM hydrogen peroxide was added to allcells for 4 h, with the exception of the h and then 0.7 mM hydrogen peroxide was added to allcells for 4 h, with the exception of the Save | Full Paper |
2014 | Langevin, SM;Houseman, EA;Accomando, WP;Koestler, DC;Christensen, BC;Nelson, HH;Karagas, MR;Marsit, CJ;Wiencke, JK;Kelsey, KT; | Leukocyte-adjusted epigenome-wide association studies of blood from solid tumor patients | Epigenetics | 24671036 | Briefly, sorted non-pathological human peripheral blood leukocytes (n = 47) were previously obtained from different anonymous individuals through AllCells, LLC. Leukocytes were Save | Full Paper |
2014 | Xiao, W;Wang, J;Li, H;Xia, D;Yu, G;Yao, W;Yang, Y;Xiao, H;Lang, B;Ma, X;Guo, X;Guan, W;Xu, H;Liu, J;Zhang, X;Ye, Z; | Fibulin-1 is epigenetically down-regulated and related with bladder cancer recurrence | BMC cancer | 25234557 | O2 maintained at 37°C. SV-HUC-1 was also purchased from ATCC and maintained in DMEM/F-12 medium. Primary human umbilical vein endothelial cell (HUVEC) and Endothelial Cell Medium were purchased from ALLCELLS, LLC (Shanghai, China). Plasmid transfecti | Full Paper |
2014 | Thorarensen, A;Banker, ME;Fensome, A;Telliez, JB;Juba, B;Vincent, F;Czerwinski, RM;Casimiro-Garcia, A; | ATP-mediated kinome selectivity: the missing link in understanding the contribution of individual JAK Kinase isoforms to cellular signaling | ACS chemical biology | 24814050 | Cryopreserved human PBMCs (no. PB005F), which were used in the IL-2, IL-10, IL-15, and IFNα assays, were purchased from Allcells (Emeryville, CA). Frozen PBMCs were thawed in a water bath (37 °C) and washed once with once with 5% FBS/RPMI-1640 | Full Paper |
2014 | Womble, TA;Green, S;Shahaduzzaman, M;Grieco, J;Sanberg, PR;Pennypacker, KR;Willing, AE; | Monocytes are essential for the neuroprotective effect of human cord blood cells following middle cerebral artery occlusion in rat | Molecular and cellular neurosciences | 24472845 | The cells (either HUCB or HPB MNC, obtained commercially from AllCells, LLC), were thawed rapidly at 37 C and transferred slowly into 10 ml of 10 mM PBS. The cells were then Save | Full Paper |
2014 | Fraser, P;Macdonald, G;Mullineux, A; | Regional Monetary Policy: An Australian Perspective | Regional Studies | This is a feature which has been under discussion and analysis for some time (for example, Tremosa-Ballcells and Pons-Novell, 2001; Mojon and Peersman, 2001; Aksoy et al., 2002; Clausen and Wohltmann, 2005; and more recently, Dow et al., 2012; and al | Full Paper | |
2014 | Liu, X;Ou, YC;Zhang, J;Ahene, A;Clark, D;Hsieh, SC;Cooper, M;Ji, C; | Pharmacokinetics and pharmacodynamics of CD4-anchoring bi-functional fusion inhibitor in monkeys | Pharmaceutical research | 24065594 | BD Biosciences (La Jolla, CA). Human PBMCs from healthy donors were purchased from AllCells (cat # PB001, Emeryville, CA) and cultured in complete medium (RPMI 1640, 10% FBS, 1% P/S, 1% L-Glutamine, 1% non-essential amino acid, 1% sodium pyruvate, 1% | Full Paper |
2014 | Wolstein, O;Boyd, M;Millington, M;Impey, H;Boyer, J;Howe, A;Delebecque, F;Cornetta, K;Rothe, M;Baum, C;Nicolson, T;Koldej, R;Zhang, J;Keech, N;Camba Colón, J;Breton, L;Bartlett, J;An, DS;Chen, IS;Burke, B;Symonds, GP; | Preclinical safety and efficacy of an anti-HIV-1 lentiviral vector containing a short hairpin RNA to CCR5 and the C46 fusion inhibitor | Molecular therapy. Methods & clinical development | 26015947 | ced overnight on RetroNectin (TaKaRa, Japan, #T100B) coated plates at various MOI, as indicated in the experimental methodology. CD34+ cells Primary human CD34+ HSPC were purchased commercially from AllCells; cells were directly isolated from granul | Full Paper |
2014 | Palasuk, J;Kamocki, K;Hippenmeyer, L;Platt, JA;Spolnik, KJ;Gregory, RL;Bottino, MC; | Bimix antimicrobial scaffolds for regenerative endodontics | Journal of endodontics | 25201643 | Introduction Eliminating and/or inhibiting bacterial growth within the root canal system has been shown to play a key role in the regenerative outcome. The aim of this study was to Save | Full Paper |
2014 | Zhang, HT;Wang, L;Ai, J;Chen, Y;He, CX;Ji, YC;Huang, M;Yang, JY;Zhang, A;Ding, J;Geng, MY; | SOMG-833, a novel selective c-MET inhibitor, blocks c-MET-dependent neoplastic effects and exerts antitumor activity | The Journal of pharmacology and experimental therapeutics | 24741075 | HUVEC cells (passage 3) were first serum starved in complete medium (AllCells) for 24 hours and treated by SOMG-833 for 72 hours in media containing 3% bovine serum albumin and Save | Full Paper |
2014 | Kimbrel, EA;Kouris, NA;Yavanian, GJ;Chu, J;Qin, Y;Chan, A;Singh, RP;McCurdy, D;Gordon, L;Levinson, RD;Lanza, R; | Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic properties | Stem cells and development | 24650034 | from fresh BM aspirates (AllCells LLC, Alameda, CA) using Histopaque 1077/Accuspin tubes (Sigma-Aldrich) or from frozen human BM mononuclear cells (AllCells LLC) from both male Save | Full Paper |
2014 | Iwata, M;Sandstrom, RS;Delrow, JJ;Stamatoyannopoulos, JA;Torok-Storb, B; | Functionally and phenotypically distinct subpopulations of marrow stromal cells are fibroblast in origin and induce different fates in peripheral blood monocytes | Stem cells and development | 24131213 | The cells were cultured in mesenchymal stromal cell (MSC) basal media with MSC stimulatory supplement according to the manufacturer's protocol (ALLCELLS), and then expanded in Save | Full Paper |
2014 | Johnston, JM;Denning, G;Moot, R;Whitehead, D;Shields, J;Le Doux, JM;Doering, CB;Spencer, HT; | High-throughput screening identifies compounds that enhance lentiviral transduction | Gene therapy | 25231175 | Fresh CD34+ cells were purchased from AllCells (Emeryville, CA, USA). Before arrival, CD34+ cells were mobilized from human bone marrow and isolated from the peripheral blood of three individual subjects. Flow cytometry was used to assess the purity | Full Paper |
2014 | Kamata, S;Miyagawa, S;Fukushima, S;Nakatani, S;Kawamoto, A;Saito, A;Harada, A;Shimizu, T;Daimon, T;Okano, T;Asahara, T;Sawa, Y; | Improvement of cardiac stem cell sheet therapy for chronic ischemic injury by adding endothelial progenitor cell transplantation: analysis of layer-specific regional cardiac function | Cell transplantation | 23562134 | Each CSC sheet was approximately 42 mm in diameter and 100 μm thick. Granulocyte colony-stimulating factor-mobilized EPCs of human origin (AllCells, MPB-017F; Emeryville, CA, USA) were labeled with 2 μM DiI-blue in vitro (Molecular Probes) (33). The | Full Paper |
2014 | Xiao, H;Li, H;Yu, G;Xiao, W;Hu, J;Tang, K;Zeng, J;He, W;Zeng, G;Ye, Z;Xu, H; | MicroRNA-10b promotes migration and invasion through KLF4 and HOXD10 in human bladder cancer | Oncology reports | 24573354 | SV-HUC-1 was also purchased from ATCC and maintained in DMEM/F-12 medium. SCaBER was purchased from AllCells, LLC (Shanghai, China) in MEM medium. Paired samples of primary BC, and lymph node metastatic tissues were obtained from patients who had und | Full Paper |
2014 | Czubak, P;Bojarska-Junak, A;Tabarkiewicz, J;Putowski, L; | A modified method of insulin producing cells' generation from bone marrow-derived mesenchymal stem cells | Journal of diabetes research | 25405207 | Human bone marrow stromal cells (MSCs) were purchased from a commercial source (AllCells; catalog number MSC-001F). This cell line was derived from one, nondiabetic Caucasian Save | Full Paper |
2014 | Gupta, MS;Nicoll, SB; | Functional nucleus pulposus-like matrix assembly by human mesenchymal stromal cells is directed by macromer concentration in photocrosslinked carboxymethylcellulose hydrogels | Cell and tissue research | 25092545 | Human MSCs were purified from bone marrow (AllCells, Emeryville, CA, USA) using enriched density centrifugation as previously described (Gupta et al. 2011; Chen et al. 2013). Briefly, a Human MSC Enrichment Cocktail (StemCell Technologies, Vancouver, | Full Paper |
2014 | Pavlides, S;Gutierrez-Pajares, JL;Iturrieta, J;Lisanti, MP;Frank, PG; | Endothelial caveolin-1 plays a major role in the development of atherosclerosis | Cell and tissue research | 24390341 | Human umbilical vein endothelial cells (HUVEC) were obtained from AllCells (AllCells, LLC, Emeryville, CA, USA). HUVEC were cultured in M199 media (Life Technologies, Grand Island, NY, USA) containing 10 % FBS (Life Technologies, Grand Island, NY, US | Full Paper |
2014 | Kiang, JG;Garrison, BR;Smith, JT;Fukumoto, R; | Ciprofloxacin as a potential radio-sensitizer to tumor cells and a radio-protectant for normal cells: differential effects on γ-H2AX formation, p53 phosphorylation, Bcl-2 production, and cell death | Molecular and cellular biochemistry | 24802382 | Human B lymphoblastoid cell line TK6 (p53+/+) and human NH32 (p53−/− of TK6 cells) were generous gifts from Dr. James Mitchell. Human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Emeryville, CA). Cells were grown in RPMI 1 | Full Paper |
2014 | Dong, B;Zhou, H;Han, C;Yao, J;Xu, L;Zhang, M;Fu, Y;Xia, Q; | Ischemia/reperfusion-induced CHOP expression promotes apoptosis and impairs renal function recovery: the role of acidosis and GPR4 | PloS one | 25343248 | Human umbilical vein endothelial cells (HUVECs), as well as supplier's recommended medium and supplement were purchased from ALLCELLS (Emeryville, California, USA). Human Save | Full Paper |
2014 | Iwata, M;Torok-Storb, B;Wayner, EA;Carter, WG; | CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine production | PloS one | 25275584 | The cells were cultured in MSC Basal media with MSC stimulatory supplement according to the manufacturer's protocol (ALLCELLS, Emeryville, CA). The cells were passaged until the Save | Full Paper |
2014 | Ayala-Lujan, JL;Vijayakumar, V;Gong, M;Smith, R;Santiago, AE;Ruiz-Perez, F; | Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytes | PloS one | 25251283 | Fresh human blood to isolate primary cells were purchased from Allcells LLC. (Alameda, Fresh heparinized blood from healthy human donors purchased from AllCells LLC. (Alameda, Save | Full Paper |
2014 | Kang, Y;Hodges, A;Ong, E;Roberts, W;Piermarocchi, C;Paternostro, G; | Identification of drug combinations containing imatinib for treatment of BCR-ABL+ leukemias | PloS one | 25029499 | , USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, USA), 100 U/ml penicillin, 100 µg/ml streptomycin (Hyclone, USA) at 37°C in 5% CO2. CD34+ hematopoietic stem cells were purchased from Allcells and maintained in StemSpan SFEM (STEMCELL T | Full Paper |
2014 | Lewis, ND;Patnaude, LA;Pelletier, J;Souza, DJ;Lukas, SM;King, FJ;Hill, JD;Stefanopoulos, DE;Ryan, K;Desai, S;Skow, D;Kauschke, SG;Broermann, A;Kuzmich, D;Harcken, C;Hickey, ER;Modis, LK; | A GPBAR1 (TGR5) small molecule agonist shows specific inhibitory effects on myeloid cell activation in vitro and reduces experimental autoimmune encephalitis (EAE) in vivo | PloS one | 24967665 | Human peripheral blood mononuclear cells isolated with leukapheresis were purchased from AllCells (Alameda, CA), frozen in 80% FBS, 10% RPMI and 10% DMSO and stored in Save | Full Paper |
2014 | Simeonov, KP;Uppal, H; | Direct reprogramming of human fibroblasts to hepatocyte-like cells by synthetic modified mRNAs | PloS one | 24963715 | ield of direct reprogramming can overcome its retroviral reliance and be translated to its ultimate applications in regenerative medicine. Materials and Methods Cell Culture CD34+ bone marrow cells (AllCells, Emeryville, CA) were received fresh and | Full Paper |
2014 | Hong, KU;Guo, Y;Li, QH;Cao, P;Al-Maqtari, T;Vajravelu, BN;Du, J;Book, MJ;Zhu, X;Nong, Y;Bhatnagar, A;Bolli, R; | c-kit+ Cardiac stem cells alleviate post-myocardial infarction left ventricular dysfunction despite poor engraftment and negligible retention in the recipient heart | PloS one | 24806457 | m (A LV) which included the infarcted and border zones, posterior LV wall (nonischemic region; P LV), and atria+right ventricle. Female human peripheral mononuclear cells (hPBMCs) were purchased from AllCells, LLC (Emeryville, CA). Aliquots of 105 hP | Full Paper |
2014 | Gañán-Gómez, I;Wei, Y;Yang, H;Pierce, S;Bueso-Ramos, C;Calin, G;Boyano-Adánez, Mdel C;García-Manero, G; | Overexpression of miR-125a in myelodysplastic syndrome CD34+ cells modulates NF-κB activation and enhances erythroid differentiation arrest | PloS one | 24690917 | Human bone marrow cells from 6 healthy individuals were obtained from AllCells (Emeryville, CA). Isolation of CD34 + cells was performed using MicroBead Kit (Miltenyi, Bergisch Save | Full Paper |
2014 | Chyu, KY;Lio, WM;Dimayuga, PC;Zhou, J;Zhao, X;Yano, J;Trinidad, P;Honjo, T;Cercek, B;Shah, PK; | Cholesterol lowering modulates T cell function in vivo and in vitro | PloS one | 24647529 | ential batch-to-batch differences in the biochemical contents in the FBS which may affect the experimental outcomes. The same experiment was performed using human peripheral blood mononuclear cells (AllCells, Emeryville, CA). T cells were isolated f | Full Paper |
2014 | Chen, J;Li, C;Wang, S; | Periodic heat shock accelerated the chondrogenic differentiation of human mesenchymal stem cells in pellet culture | PloS one | 24632670 | nd Methods All reagents and chemicals without manufacture labels were purchased from Sigma-Aldrich (St Louis, MO). hMSC Isolation and Characterization Human MSCs were isolated from bone marrow (BM) (AllCells LLC, Berkeley, CA) as described previousl | Full Paper |
2014 | Wang, J;Guo, X;Lui, M;Chu, PJ;Yoo, J;Chang, M;Yen, Y; | Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitro | PloS one | 24465489 | This study was conducted using data obtained from 70 fresh hPB and 30 fresh hBM samples purchased from AllCells, LLC. A list of the reagents and antibodies used in this study is Save | Full Paper |
2014 | Beckwith, R;Worden, E;Estrin, E;Martin, A; | Reconstitution of the 26S Proteasome Reveals Functional Asymmetries in its Heterohexameric AAA+ Unfoldase | Biophysical Journal | Proteins are complex and versatile macromolecules that are integral components of allcells. Among other things, proteins are responsible for structural organization, signalingand Save | Full Paper | |
2014 | Aikawa, N;Kunisato, A;Nagao, K;Kusaka, H;Takaba, K;Ohgami, K; | Detection of thalidomide embryotoxicity by in vitro embryotoxicity testing based on human iPS cells | Journal of pharmacological sciences | 24451995 | The hiPSCs were derived from human bone marrow mononuclear cells which were purchased from AllCells (Emeryville, CA, USA) (11). Human dermal fibroblasts isolated from adult Save | Full Paper |
2014 | Chen, JK;Li, ZP;Liu, YZ;Zhao, T;Zhao, XB;Ni, M;Jiang, GJ;Shen, FM; | Activation of alpha 7 nicotinic acetylcholine receptor protects mice from radiation-induced intestinal injury and mortality | Radiation research | 24828112 | Radiation-induced gastrointestinal syndrome occurs when the body is exposed to a high dose of radiation. Currently, safe and effective radioprotectants are not available. Apoptosis Save | Full Paper |
2014 | Lee, M;Jeong, SY;Ha, J;Kim, M;Jin, HJ;Kwon, SJ;Chang, JW;Choi, SJ;Oh, W;Yang, YS;Kim, JS;Jeon, HB; | Low immunogenicity of allogeneic human umbilical cord blood-derived mesenchymal stem cells in vitro and in vivo | Biochemical and biophysical research communications | 24657442 | Abstract Evaluation of the immunogenicity of human mesenchymal stem cells (MSCs) in an allogeneic setting during therapy has been hampered by lack of suitable models due to Save | Full Paper |
2014 | Shima, T;Miyamoto, T;Kikushige, Y;Yuda, J;Tochigi, T;Yoshimoto, G;Kato, K;Takenaka, K;Iwasaki, H;Mizuno, S;Goto, N;Akashi, K; | The ordered acquisition of Class II and Class I mutations directs formation of human t(8;21) acute myelogenous leukemia stem cell | Experimental hematology | 25101977 | Human marrow was purchased from AllCells (Emeryville, CA). Informed consent was obtained from all patients. The Institutional Review Board of Kyushu University Hospital (Fukuoka, Save | Full Paper |
2014 | Iancu-Rubin, C;Mosoyan, G;Glenn, K;Gordon, RE;Nichols, GL;Hoffman, R; | Activation of p53 by the MDM2 inhibitor RG7112 impairs thrombopoiesis | Experimental hematology | 24309210 | Human MK were generated in a two-step liquid culture system from bone marrow (BM)-derived CD34+ cells (AllCells, Emeryville, CA, USA). Cells were suspended in IMDM-based MK differentiation medium [6] for 7 days then allowed to mature for 7-9 addition | Full Paper |
2014 | Kornblau, SM;Cohen, AC;Soper, D;Huang, YW;Cesano, A; | Age-related changes of healthy bone marrow cell signaling in response to growth factors provide insight into low risk MDS | Cytometry. Part B, Clinical cytometry | 24106013 | patients aged 54 to 82 years undergoing planned orthopedic procedures at Williamson Medical Center in Tennessee. The second (“younger”) healthy sample set consisted of 6 cryopreserved BMMC obtained from donors aged 23 to 43 years, purchased from a co | Full Paper |
2014 | Barrionuevo, M; | Society of Toxicology (SOT) 5st Annual Meeting and ToxExpo, San Francisco, CA, USA, March 11-15, 2012 | Drugs of the Future | 283 on human myeloid, erythroid and mesenchymal lineages for its potential to protect marrow progenitor cells from radiation damage was discussed by Emer Clarke from ReachBio. Save | Full Paper | |
2014 | Fu, Y;Chen, YL;Herve, M;Gu, F;Shi, PY;Blasco, F; | Development of a FACS-based assay for evaluating antiviral potency of compound in dengue infected peripheral blood mononuclear cells | Journal of virological methods | 24140514 | Isolated cryopreserved human PBMCs were obtained from AllCells, LLC. The PBMCs were obtained with informed consent and experiments involving PBMCs were approved by Save | Full Paper |
2014 | Tilton, CA;Tabler, CO;Lucera, MB;Marek, SL;Haqqani, AA;Tilton, JC; | A combination HIV reporter virus system for measuring post-entry event efficiency and viral outcome in primary CD4+ T cell subsets | Journal of virological methods | 24025341 | Peripheral blood mononuclear cells (PBMCs) and CD4+ T cells were obtained from leukopheresis from ALLCELLS, LLC on five healthy donors. All subjects for this study were de- Save | Full Paper |
2014 | Shiheido, H;Kitagori, K;Sasaki, C;Kobayashi, S;Aoyama, T;Urata, K;Oku, T;Hirayama, Y;Yoshitomi, H;Hikida, M;Yoshifuji, H;Mimori, T;Watanabe, T;Shimizu, J; | Human T cells expressing BEND3 on their surface represent a novel subpopulation that preferentially produces IL-6 and IL-8 | Immunity, inflammation and disease | 25400923 | , #16-15 Abs, and 7AAD, and BEND3+ or BEND3− subpopulations in CD4+ or CD8+ T cells were sorted using a flow cytometer (FACSAria, BD Biosciences, San Jose, CA). Cord blood cells were purchased from ALLCELLS, and Jurkat [25] cells were from the Americ | Full Paper |
2014 | Cheng, ZJ;Garvin, D;Paguio, A;Moravec, R;Engel, L;Fan, F;Surowy, T; | Development of a robust reporter-based ADCC assay with frozen, thaw-and-use cells to measure Fc effector function of therapeutic antibodies | Journal of immunological methods | 25086226 | PBMCs from different donors were from AllCells. PNGase F, Magne™ Protein G Beads, Bio-Glo™ Luciferase Assay System and CytoTox 96® Non-Radioactive Cytotoxicity Assay were from Promega | Full Paper |
2014 | Furuya, T;Sá, JM;Chitnis, CE;Wellems, TE;Stedman, TT; | Reticulocytes from cryopreserved erythroblasts support Plasmodium vivax infection in vitro | Parasitology international | 24291603 | Plasmodium vivax is the most widely distributed human malaria parasite. Despite its importance, both clinical research and basic research have been hampered by lack of a convenient Save | Full Paper |
2014 | Meissner, TB;Mandal, PK;Ferreira, LM;Rossi, DJ;Cowan, CA; | Genome editing for human gene therapy | Methods in enzymology | 25398345 | CD34 + cells from G-CSF mobilized peripheral blood were purchased from AllCells. CD34 + cells were cotransfected with CRISPR/Cas9 expression plasmids using Amaxa Save | Full Paper |
2014 | Wilson, EM;Bial, J;Tarlow, B;Bial, G;Jensen, B;Greiner, DL;Brehm, MA;Grompe, M; | Extensive double humanization of both liver and hematopoiesis in FRGN mice | Stem cell research | 25310256 | rich Chemicals; non-platable human hepatocytes (> 70% viable by trypan blue staining) were purchased from BioreclamationIVT (catalog# F00995); human frozen bone marrow CD34+ cells were purchased from AllCells (Catalog# ABM017F); HCM media was purchas | Full Paper |
2014 | Yourek, G;Xin, X;Reilly, GC;Mao, JJ; | Infiltration of mesenchymal stem cells into PEGDA hydrogel | Bio-medical materials and engineering | 25201394 | Human MSCs were isolated from commercially available whole bone marrow (AllCells, Berkeley, CA) using "RosetteSep " (StemCell Technologies; Vancouver, BC) according to the Save | Full Paper |
2014 | Chang, TP;Vancurova, I; | Bcl3 regulates pro-survival and pro-inflammatory gene expression in cutaneous T-cell lymphoma | Biochimica et biophysica acta | 25089799 | The advanced stages of cutaneous T cell lymphoma (CTCL) are characterized not only by decreased levels of pro-inflammatory cytokines, resulting in high susceptibility to infections, Save | Full Paper |
2014 | Li, J;Sun, W;Subrahmanyam, PB;Page, C;Younger, KM;Tiper, IV;Frieman, M;Kimball, AS;Webb, TJ; | NKT Cell Responses to B Cell Lymphoma | Medical sciences (Basel, Switzerland) | 24955247 | tained from commercial vendors. Specifically, buffy coats were purchased from Biological Specialty Corporation and peripheral blood from two different, newly diagnosed MCL patients was purchased from AllCells, LLC (Alameda, CA, USA). PBMCs were isola | Full Paper |
2014 | Gitschier, HJ;Bergeron, AB;Randle, DH; | Label-free cell-based dynamic mass redistribution assays | Current protocols in chemical biology | 24652622 | Human umbilical vein endothelial cells (Corning, cat. no. 354151) Peripheral blood mononuclear cells (AllCells, cat. no. PB006F) Appropriate cell culture/differentiation medium with supplements, e.g.: IMDM, KnockOut DMEM/F-12 with Stempro supplement, | Full Paper |
2014 | Vij, R;Mazumder, A;Klinger, M;O'Dea, D;Paasch, J;Martin, T;Weng, L;Park, J;Fiala, M;Faham, M;Wolf, J; | Deep sequencing reveals myeloma cells in peripheral blood in majority of multiple myeloma patients | Clinical lymphoma, myeloma & leukemia | 24629890 | The remaining 13 paired bone marrow and peripheral blood samples were purchased from a commercial source (AllCells, Emeryville, CA). Save | Full Paper |
2014 | DeClerck, Y; | Environment-Mediated Drug Resistance in Neuroblastoma | U.S. Army Medical Research Material Command | Human BMMSC were purchased from AllCells LLC. Monocytes of normal healthy donors were obtained from peripheral blood and separated by Ficoll density gradient centrifugation Save | Full Paper | |
2014 | Kornblau, S; | The Use of “Omics” to Guide the Selection of Targeted Therapy | Targeted Therapy of Acute Myeloid Leukemia | If the normal HSC frequency is 1:100,000, then multiply this by 10. For our protein work, we have therefore used a CD34+ cell from a normal bone marrow as our standard comparator (we have successfully used CD34+ cells from AllCells for our RPPA work) | Full Paper | |
2014 | 허운학, ; | Concurrent treatment with capecitabine and radiosurgery in the mouse glioblastoma model | Thesis | AllCells were cultured for two to three passages in Dulbecco's modified Eagle's medium (WelGENE, Daegu, Korea) containing 10% fetal bovine serum and 1% antibiotic-antimycotic Save | Full Paper | |
2014 | Zaidi-Merchant, SS; | The role of let-7c/miR-125b/miR-99a in the hematopoietic development in the human bone marrow | Thesis | Normal human bone marrow samples were obtained from healthy donors via the UCLA Pathology Tissue Core, Cincinnati Children’s Hospital, or ALLCELLS according to guidelines approved by UCLA Institutional Review Board. Fresh BM samples were Ficoll-Paque | Full Paper | |
2014 | Holm, HH; | A CUDA Back-End for the Equelle Compiler. | Thesis | The function Gradient takes a Collection Of Scalar On AllCells() as input and compute the discrete If u is a variable defined as Collection Of Scalar On AllCells() then the following line is Save | Full Paper | |
2014 | Oien, N; | Erythrocyte Carriers: Tool Development for Erythrocyte Mediated Diagnostic and Drug Delivery | Thesis | Peripheral erythrocytes were purchased from AllCells. Dialysis cassettes were purchased Peripheral red blood cells were purchased from AllCells. 8-(4-chlorophenylthio) adenosine-3′ Save | Full Paper | |
2014 | Nguyen, L; | LONG WAVELENGTH FLUORESCENT REPORTERS FOR PROTEIN KINASES AND DESIGN OF LIGHT-ACTIVATABLE BIOAGENTS | Thesis | H89 and KT5720 were purchased from Sigma-Aldrich and Calbiochem (EMD), respectively. Peripheral red blood cells were purchased from AllCells. 8-(4-chlorophenylthio) adenosine-3′,5′-cyclic monophosphorothioate (CPT-cAMP), ATP, and cAMP were purchased | Full Paper | |
2014 | Rathbone, E; | Inhibitory and Immunomodulatory Properties of Human Choroid Plexus Epithelial Cells | Thesis | Pooled BMSC, ADSC (both Lonza, Basel, Switzerland), DPSC (AllCells L.L.C., Alameda, CA, USA) and AMSC were cultured in high glucose Dulbecco’s Modified Eagle Medium (HG-DMEM; Biosera, Boussens, France) supplemented with 10% foetal bovine serum (FBS), | Full Paper | |
2014 | Suganyaa, MV;Deepa, MKRS;Vinothini, MG; | Operating the SRAM Cell Architecture with the High Efficient and Reduced Memory Size | ijaist.com | It has a multiplicative effect; allcells in the array are drawing this power. The CMOS 8T cellrequires about 60 times more static power than SRAM cells inthis study. The SRAM 8T cell Save | Full Paper | |
2014 | Yu, Y; | Energy prediction of electric floor radiation systems using a new integrated modeling approach | ASHRAE Transactions | In this figure, PMV Onezone and PPD Onezone represent the PMV and PPD values when the one-zone (multi-zone) model is applied and PMV Allcells and PPD Allcells represent the Save | Full Paper | |
2014 | Xiao, F; | Genomic study of familial breast cancer-genomic instability, classification and predisposition | Thesis | The cells for the study were purchased from AllCells LLC (http://www.allcells.com/, Emeryville, California), which supplies human blood cells from donors for research and the company has its full IRB system (Biomed IRB). The signed written consent fo | Full Paper | |
2014 | Perignon, MC; | A Rolling Stone Gathers No Moss: Modeling the Geomorphic Impacts of Floods in the Southern Rocky Mountain Region, USA | Thesis | (Top, right) Non-local survival function for sediment entering the system at the mouth of the fan, for allcells with ow depth greater than 1 cm. (Bottom, right) Non-local probability of Save | Full Paper | |
2014 | Suwannatat, P; | A Visual Analysis Toolkit for Microscopic Image Mosaics of Retinal Astrocytes | Thesis | 5 The cell locations are kept at allcells.txt under the data directory. as a gray cell among multi-hue neighbors with 100% saturations. with a "new cell here" command of the mouse Save | Full Paper | |
2014 | Johnson, SM; | Extracellular matrix improves mesenchymal stem cell growth and maintenance of stemness in serum free medium | Thesis | Freshly isolated human bone marrow mononuclear cells, containing BM-MSCs (primary cells) from 20-25 year old donors were purchased from ALLCELLS (Emeryville, CA). Greater Save | Full Paper | |
2014 | Tsai, HC; | Molecular Mechanism in the Regulation of Human Th17 Cell Differentiation and Function | Thesis | The use of human cells in the study was periodically reviewed and approved by the Human Subject Research Review Committee (University of California, Davis). PBMCs were purchased from Allcells. LLC. Samples used in a single experiment were from differ | Full Paper | |
2014 | Spinler, KR; | Membrane forces and key protein determinants of hematopoietic cell function: Lamins and myosin-II in hematopoiesis and CD47 in immunotherapy of cancer | Thesis | Fresh purified bone marrow-derived human CD34+ cells and total mononuclear cells were obtained from either the Penn Xenograft Core Facility or AllCells (Emeryville, CA). Cells from at least five different donors were used in this study. Purity of th | Full Paper | |
2014 | Amir, ED; | viSNE and Wanderlust, two algorithms for the visualization and analysis of high-dimensional single-cell data | Thesis | Fresh, Ficoll-enriched human bone marrow was obtained from healthy donors from AllCells, Inc. (Emeryville, CA). Samples were obtained with informed consent in accordance with the Declaration of Helsinki and with accordance with Stanford University’s | Full Paper | |
2014 | Qin, S; | The use of biomaterials for cell function enhancement: acceleration of fibroblast migration and promotion of stem cell proliferation | Thesis | Human cord blood CD34+ cells were purchased from AllCells LLC and cultured in StemSpan SFEM media, 10% FBS, 100 ng/ml each of human SCF, TPO, and Flt-3L. Cells were infected with ICD-Notch or GFP control lentiviral particles then plated on hydrogels | Full Paper | |
2014 | Krzeszinski, JY;Wei, W;Huynh, H;Jin, Z;Wang, X;Chang, TC;Xie, XJ;He, L;Mangala, LS;Lopez-Berestein, G;Sood, AK;Mendell, JT;Wan, Y; | miR-34a blocks osteoporosis and bone metastasis by inhibiting osteoclastogenesis and Tgif2 | Nature | 25043055 | Human PBMN cells (ReachBio) were differentiated into osteoclasts in α-MEM containing 10% FBS, 25 ng m1 −l MCSF, 50 ng m1 −l hRANKL, 1 μM Dexamethasome and 1 μM Save;odeoxyuridine (BrdU) cell proliferation assay kit (GE Healthcare) as described27. O | Full Paper |
2014 | Shah, N;Cortes, J;Martinelli, G;Smith, B;Clarke, E;Copland, M;Strauss, L;Talpaz, M; | Dasatinib Plus Smoothened (SMO) Inhibitor BMS-833923 in Chronic Myeloid Leukemia (CML) with Resistance or Suboptimal Response to a Prior Tyrosine Kinase Inhibitor (TKI): Phase I Study CA180323 | Blood | University of California, San Francisco, San Francisco, CA++The University of Texas MD Anderson Cancer Center, Houston, TX++University of Bologna, Bologna, Italy++Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD++ReachBio LLC | Full Paper | |
2014 | Chattopadhyay, R;Dyukova, E;Singh, NK;Ohba, M;Mobley, JA;Rao, GN; | Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C ε-mediated zona occludens-1 phosphorylation at threonine 770/772 | The Journal of biological chemistry | 24338688 | The human peripheral blood CD14+ monocytes were purchased from ReachBio LLC (Seattle, WA), suspended in RPMI 1640 medium containing 50 μm 2-mercaptoethanol, 100 IU/ml penicillin, and 100 μg/ml streptomycin, and used for transmigration assay. To colle | Full Paper |
2014 | Winter, JM;Jacobson, P;Bullough, B;Christensen, AP;Boyer, M;Reems, JA; | Long-term effects of cryopreservation on clinically prepared hematopoietic progenitor cell products | Cytotherapy | 24910385 | CFC assays were performed 21, 22 with either commercially available MethoCult (StemCell Technologies Inc, Vancouver, Canada) or ColonyGel (ReachBio, LLC, Seattle, WA, USA) that had been internally validated to show comparable results. Briefly, HPC(A) | Full Paper |
2014 | Chandrasekaran, D;Nakamoto, B;Watts, KL;Kiem, HP;Papayannopoulou, T; | Modeling promising nonmyeloablative conditioning regimens in nonhuman primates | Human gene therapy | 24937231 | Transduced, mock-transduced, and BM white blood cell samples from transplanted macaques were cultured in semisolid methylcellulose medium for 12-14 days in the presence of rhIL-3, recombinant human erythropoietin (rhEPO), rhSCF, and recombinant human | Full Paper |
2014 | Cook, GJ;Caudell, DL;Elford, HL;Pardee, TS; | The efficacy of the ribonucleotide reductase inhibitor Didox in preclinical models of AML | PloS one | 25402485 | bated as indicated above for 24 hours with a titration of Didox. Cells were washed with PBS and resuspended in IMDM supplemented with 20% FBS, and placed in ColonyGel High Cytokine Formulation media (ReachBio). Experiments were performed in triplicat | Full Paper |
2014 | Byon, JC;Padilla, SM;Papayannopoulou, T; | Deletion of Dicer in late erythroid cells results in impaired stress erythropoiesis in mice | Experimental hematology | 24971698 | Colony Forming Assay Peripheral blood, spleen and bone marrow mononuclear cells were plated in methylcellulose containing hematopoietic growth factors (ReachBio, WA) as previously described [17] | Full Paper |
2014 | Li, X;Xu, X;Ji, T;Liu, Z;Gu, M;Hoang, BH;Zi, X; | Dietary feeding of Flavokawain A, a Kava chalcone, exhibits a satisfactory safety profile and its association with enhancement of phase II enzymes in mice | Toxicology reports | 25379458 | ated, the yield and viability of cells were determined by Trypan blue exclusion method. A total number of 2 × 104 cells were seeded in a 6-well plate containing Colony Gel 1201 Mouse Base Medium (Reachbio), and then treated with FKA and Adriamycin a | Full Paper |
2014 | Erythroid, IVEOS; | Progenitor Populations And Analogous Human Stress Erythroid Progenitors | Thesis | Human bone marrow mononuclear cells were purchased from Reachbio [26-‐200] and cultured in the stress erythropoiesis medium described above. On the indicated days, cells were Save | Full Paper | |
2014 | Smuda, CA; | The Transcriptional Regulator Ikaros and Control of Mast Cell and Basophil Development | Thesis | We took advantage of the sorting strategies developed for quantitation of progenitor subtypes to isolate these populations for further analysis in solid culture media. IL-3 (5 ng/mL) and SCF (12.5 ng/mL) was added ColonyGel 1201 base methylcellulose | Full Paper | |
2013 | Singh, R;Low, ET;Ooi, LC;Ong-Abdullah, M;Ting, NC;Nagappan, J;Nookiah, R;Amiruddin, MD;Rosli, R;Manaf, MA;Chan, KL;Halim, MA;Azizi, N;Lakey, N;Smith, SW;Budiman, MA;Hogan, M;Bacher, B;Van Brunt, A;Wang, C;Ordway, JM;Sambanthamurthi, R;Martienssen, RA; | The oil palm SHELL gene controls oil yield and encodes a homologue of SEEDSTICK | Nature | 23883930 | We thank Johannes Jansen (PRI, Wageningen, Netherlands) for assistance with JoinMap, and Beijing Genome Institute, The Genome Institute at Washington University, Tufts University Core Facility and the Arizona Genome Institute for mapping and genome s | Full Paper |
2013 | Ong, CT;Van Bortle, K;Ramos, E;Corces, VG; | Poly(ADP-ribosyl)ation regulates insulator function and intrachromosomal interactions in Drosophila | Cell | 24055367 | We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology for their help in performing Illumina sequencing of ChIP-Seq samples. This work was supported by U.S. Public Health Service Award R01 GM035463 from the Nat | Full Paper |
2013 | Mukhopadhyay, S;Wen, X;Ratti, N;Loktev, A;Rangell, L;Scales, SJ;Jackson, PK; | The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signaling | Cell | 23332756 | For the ISH experiments using radiolabeled probes, custom service from Phylogeny Inc. (Columbus, OH) was used. Both antisense and sense radiolabeled riboprobes were synthesized in vitro according to the manufacturer’s specifications (Ambion) and labe | Full Paper |
2013 | Locke, D;Bernstein, S;Lynch, F;Siami-Namini, K;Walling, J;Yonker, T;Yarranton, G; | An IHC Screen For EphA3 Positive FFPE Tumors | Blood | November 15, 2013. An IHC Screen For EphA3 Positive FFPE Tumors. Darren Locke, PhD Darren Locke, PhD. 1. QualTek Molecular Laboratories, Newtown, USA,. 1. Search for other works by this author on: This Site. PubMed. Google | Full Paper | |
2013 | Yang, J;Sung, E;Donlin-Asp, PG;Corces, VG; | A subset of Drosophila Myc sites remain associated with mitotic chromosomes colocalized with insulator proteins | Nat Commun | 23403565 | ChIP was performed with ~4 × 107 cells. Cells were cross-linked with 1% formaldehyde for 10 min at room temperature. Nuclear lysates were sonicated to generate 200– 1000 bp DNA fragments. ChIP was then performed with 6 μL of Drosophila α-Myc antibody | Full Paper |
2013 | Day, K;Waite, LL;Thalacker-Mercer, A;West, A;Bamman, MM;Brooks, JD;Myers, RM;Absher, D; | Differential DNA methylation with age displays both common and dynamic features across human tissues that are influenced by CpG landscape | Genome Biol. | 24034465 | ;... samples All tissue samples were collected according to protocols and guidelines at their respective institutions. Iron deficient anemia buffy coat samples were commercially available and collected by Conversant Bio (Huntsville, AL, USA). Blood D | Full Paper |
2013 | Kellner, WA;Van Bortle, K;Li, L;Ramos, E;Takenaka, N;Corces, VG; | Distinct isoforms of the Drosophila Brd4 homologue are present at enhancers, promoters and insulator sites | Nucleic Acids Res. | 23945939 | Illumina adaptors (Illumina Cat# PE-102–1001) were titrated according to prepared DNA ChIP sample concentration and ligated with T4 ligase (NEB Cat# M0202S). Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (N | Full Paper |
2013 | Xing, F;Kobayashi, A;Okuda, H;Watabe, M;Pai, SK;Pandey, PR;Hirota, S;Wilber, A;Mo, YY;Moore, BE;Liu, W;Fukuda, K;Iiizumi, M;Sharma, S;Liu, Y;Wu, K;Peralta, E;Watabe, K; | Reactive astrocytes promote the metastatic growth of breast cancer stem-like cells by activating Notch signalling in brain | EMBO Mol Med | 23495140 | ... ocyte (UC1) was a kind gift from Dr Russell Piper (University of California-San Francisco). Primary human breast tumour cells which maintained in xenograft tumour of NOD/SCID mouse were obtained from Conversant Biologics, Inc. shRNA-expressing le | Full Paper |
2013 | Park, S;Balasubramaniam, V;Sastry, S; | Estimating pressure induced changes in vegetable tissue using in situ electrical conductivity measurement and instrumental analysis | Journal of Food Engineering | Pittsfield, MA, USA). The ends of the copper wires were soldered to the high pressure electrical feed through and electrically insulated using polyolefin heat shrink tubing (Q2-Z-1/8, Qualtek Electronics, Mentor, OH, USA). A type | Full Paper | |
2013 | Okuda, H;Xing, F;Pandey, PR;Sharma, S;Watabe, M;Pai, SK;Mo, YY;Iiizumi-Gairani, M;Hirota, S;Liu, Y;Wu, K;Pochampally, R;Watabe, K; | miR-7 suppresses brain metastasis of breast cancer stem-like cells by modulating KLF4 | Cancer Res. | 23384942 | mixture. Breast tumor cells that were directly transplanted to animal only one generation without in vitro culture were obtained from Conversant Biologics. Stem cell populations were isolated as described previously (14). Iso ;... odium pyruvate | Full Paper |
2013 | Sah, S;Chen, L;Houghton, J;Kemppainen, J;Marko, AC;Zeigler, R;Latham, GJ; | Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsies | Genome Med | 24001039 | Seventy-six FFPE blocks containing thyroid tumor biopsies were purchased from Asterand (Detroit, MI, USA). The remaining 39 FFPE tissue specimens were processed from colorectal cancer resections purchased from Folio Biosciences (Columbus, OH, USA) [1 | Full Paper |
2013 | Soria, JC;Massard, C;Lazar, V;Ozoux, ML;Mery-Mignard, D;Deslandes, A;Tolcher, AW; | A dose finding, safety and pharmacokinetic study of AVE1642, an anti-insulin-like growth factor-1 receptor (IGF-1R/CD221) monoclonal antibody, administered as a single agent and in combination with docetaxel in patients with advanced solid tumours | Eur. J. Cancer | 23485230 | Tumour tissue: When possible, tumour tissue was collected before AVE1642 administration and at d21-d22 of cycle 1 to evaluate IGF-1R expression and signalling pathway markers, using validated immunohistochemistry assays, in Qualtek (USA) | Full Paper |
2013 | Rafaelsen, SH;Raeder, H;Fagerheim, AK;Knappskog, P;Carpenter, TO;Johansson, S;Bjerknes, R; | Exome sequencing reveals FAM20c mutations associated with fibroblast growth factor 23-related hypophosphatemia, dental anomalies, and ectopic calcification | J. Bone Miner. Res. | 23325605 | Genomic DNA was purified from blood using the QiaSymphony system (Qiagen, Hilden, Germany). Whole‐genome single‐nucleotide polymorphism (SNP) genotyping was performed with Genome‐Wide Human SNP 6.0 array (Affymetrix, Santa Clara, CA, USA). Whole‐exom | Full Paper |
2013 | Soler, VJ;Tran-Viet, KN;Galiacy, SD;Limviphuvadh, V;Klemm, TP;St Germain, E;Fournié, PR;Guillaud, C;Maurer-Stroh, S;Hawthorne, F;Suarez, C;Kantelip, B;Afshari, NA;Creveaux, I;Luo, X;Meng, W;Calvas, P;Cassagne, M;Arné, JL;Rozen, SG;Malecaze, F;Young, TL; | Whole exome sequencing identifies a mutation for a novel form of corneal intraepithelial dyskeratosis | J. Med. Genet. | 23349227 | Seven micrograms of DNA were submitted to Hudson Alpha Laboratories (Huntsville, Alabama, USA) (http://www.hudsonalpha. org/) for deep sequencing processing to achieve an average coverage of 50× read depth. The libraries were prepared according to ma | Full Paper |
2013 | Handorf, CR;Kulkarni, A;Grenert, JP;Weiss, LM;Rogers, WM;Kim, OS;Monzon, FA;Halks-Miller, M;Anderson, GG;Walker, MG;Pillai, R;Henner, WD; | A multicenter study directly comparing the diagnostic accuracy of gene expression profiling and immunohistochemistry for primary site identification in metastatic tumors | Am. J. Surg. Pathol. | 23648464 | meeting the criteria were obtained as archived formalin-fixed, paraffin-embedded specimens from 4 institutions: the University of Tennessee (UT); Virginia Commonwealth University (VCU); Indiana University-Purdue University, Indianapolis (IUPUI); and | Full Paper |
2013 | Majumdar, S;Gong, EM;Di Vizio, D;Dreyfuss, J;Degraff, DJ;Hager, MH;Park, PJ;Bellmunt, J;Matusik, RJ;Rosenberg, JE;Adam, RM; | Loss of Sh3gl2/endophilin A1 is a common event in urothelial carcinoma that promotes malignant behavior | Neoplasia | 23814487 | ILMN_1661491 [20]. Expression of Sh3gl2 protein was evaluated using commercially available bladder cancer tissue microarrays from either Folio Biosciences (Columbus, OH) or US Biomax, Inc (Rockville, MD). Specific immunohistochemical | Full Paper |
2013 | Bowman, GL;Dodge, HH;Mattek, N;Barbey, AK;Silbert, LC;Shinto, L;Howieson, DB;Kaye, JA;Quinn, JF; | Plasma omega-3 PUFA and white matter mediated executive decline in older adults | Front Aging Neurosci | 24379780 | Dr. Quinn has received honoraria for speaking from Pfizer, Novartis, and Forrest and for consulting from Phylogeny, Inc. Dr. Quinn is a co-inventor on a patent for the use of DHA for the treatment of Alzheimer's disease. Dr. Quinn receives compensati | Full Paper |
2013 | Allaire, PD;Seyed Sadr, M;Chaineau, M;Seyed Sadr, E;Konefal, S;Fotouhi, M;Maret, D;Ritter, B;Del Maestro, RF;McPherson, PS; | Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migration | J. Cell. Sci. | 23264734 | Human brain tumor samples were obtained from the Montreal Neurological Institute Brain Tumor Tissue Bank. The experimental procedures were approved by the ethics committee of the Montreal Neurological Institute. The breast and squamous cell carcinoma | Full Paper |
2013 | Bohbot, JD;Durand, NF;Vinyard, BT;Dickens, JC; | Functional Development of the Octenol Response in Aedes aegypti | Front Physiol | 23471139 | We wish to thank the following individuals: Dr. Shawn E. Levy and Nripesh Prasad at the Genomic Services Lab at HudsonAlpha Institute for Biotechnology for Illumina sequencing and data analyses; Dr. Gary Bauchan (The Electron and Confocal Microscope | Full Paper |
2013 | Premkumar, DR;Jane, EP;Agostino, NR;DiDomenico, JD;Pollack, IF; | Bortezomib-induced sensitization of malignant human glioma cells to vorinostat-induced apoptosis depends on reactive oxygen species production, mitochondrial dysfunction, Noxa upregulation, Mcl-1 cleavage, and DNA damage | Mol. Carcinog. | 22086447 | time of tumor resection. We also obtained glioblastoma multiforme (GBM) primary cells from Conversant Biologics (Huntsville, AL), which were cultured in DMEM/F12 (Invitrogen, Carlsbad, CA). The established malignant glioma | Full Paper |
2013 | Kinder, M;Greenplate, AR;Grugan, KD;Soring, KL;Heeringa, KA;McCarthy, SG;Bannish, G;Perpetua, M;Lynch, F;Jordan, RE;Strohl, WR;Brezski, RJ; | Engineered protease-resistant antibodies with selectable cell-killing functions | J. Biol. Chem. | 23986451 | Immunohistochemistry. All immunohistochemistry was performed by QualTek Molecular Laboratories (Newtown, PA). Four-micrometer sections were dewaxed through four changes of xylene (5 min each) followed by a graded alcohol series to distilled water | Full Paper |
2013 | North, GB;Lynch, FH;Maharaj, FD;Phillips, CA;Woodside, WT; | Leaf hydraulic conductance for a tank bromeliad: axial and radial pathways for moving and conserving water | Front Plant Sci | 23596446 | Full Paper | |
2013 | Park, S;Balasubramaniam, V;Sastry, S;Lee, J; | Pressure–ohmic–thermal sterilization: A feasible approach for the inactivation of Bacillus amyloliquefaciens and Geobacillus stearothermophilus spores | Innovative Food Science & Emerging Technologies | Each soldered part was electrically insulated using heat shrink tubing (Q2-F-1/16-01-QB6IN- 36, Qualtek, Mentor, OH, USA) and epoxy (2-t ® epoxy clear, Devcon, Danvers, MA, USA) to prevent short circuits and current loss to the pressure-transmitting | Full Paper | |
2013 | Onsum, MD;Geretti, E;Paragas, V;Kudla, AJ;Moulis, SP;Luus, L;Wickham, TJ;McDonagh, CF;MacBeath, G;Hendriks, BS; | Single-cell quantitative HER2 measurement identifies heterogeneity and distinct subgroups within traditionally defined HER2-positive patients | Am. J. Pathol. | 24035511 | Patient Samples. Sections (5 μm thick) of a breast disease spectrum TMA were obtained from Folio Biosciences (Powell, OH) (catalog number ARY-HH0137). Duplicate 1-mm tissue cores from 48 patients were represented on the TMA | Full Paper |
2013 | Liu, S;Wang, X;Sun, F;Zhang, J;Feng, J;Liu, H;Rajendran, KV;Sun, L;Zhang, Y;Jiang, Y;Peatman, E;Kaltenboeck, L;Kucuktas, H;Liu, Z; | RNA-Seq reveals expression signatures of genes involved in oxygen transport, protein synthesis, folding, and degradation in response to heat stress in catfish | Physiol. Genomics | 23632418 | ;Login to your account. Institutional Login. Sign In / Register. PHYSIOLOGY.ORG Skip main navigationClose Drawer MenuOpen Drawer Menu Home: Journals: AJP-Cell; AJP-Endo; AJP-GI; AJP-Heart; AJP-Lung; AJP-Regu; AJP-Renal; ; For each group, equal | Full Paper |
2013 | Kauffman, AL;Gyurdieva, AV;Mabus, JR;Ferguson, C;Yan, Z;Hornby, PJ; | Alternative functional in vitro models of human intestinal epithelia | Front Pharmacol | 23847534 | n Majewski, Jason Ekert, and Kimberly Reese for help with iPSC maintenance culture. We also acknowledge the outstanding quality of immunohistochemistry performed by Steve Bernstein and Marko Sldanov, Qualtek Molecular Labs, Goleta, CA. References Al | Full Paper |
2013 | Lawley, B;Sims, IM;Tannock, GW; | Whole-transcriptome shotgun sequencing (RNA-seq) screen reveals upregulation of cellobiose and motility operons of Lactobacillus ruminis L5 during growth on tetrasaccharides derived from barley β-glucan | Appl. Environ. Microbiol. | 23851085 | Genomic comparisons were made in the expectation of understanding differential utilization of tetrasaccharides by L. ruminis strains. Paired-end reads were mapped to the publically available L. ruminis genomes: ATCC 25644 (did not utilize tetrasaccha | Full Paper |
2013 | Wang, R;Sun, L;Bao, L;Zhang, J;Jiang, Y;Yao, J;Song, L;Feng, J;Liu, S;Liu, Z; | Bulk segregant RNA-seq reveals expression and positional candidate genes and allele-specific expression for disease resistance against enteric septicemia of catfish | BMC Genomics | 24373586 | ;... erformed using KAPA Library Quant Kits (Kapa Biosystems, USA) and dilution, the library were clustered 3 in one lane and sequenced on a Hiseq 2500 instrument with 100 bp paired end (PE) reads at the HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2013 | Xu, J;Li, Q;Xu, L;Wang, S;Jiang, Y;Zhao, Z;Zhang, Y;Li, J;Dong, C;Xu, P;Sun, X; | Gene expression changes leading extreme alkaline tolerance in Amur ide (Leuciscus waleckii) inhabiting soda lake | BMC Genomics | 24094069 | ;... ). Equal amounts of the high quality RNA samples from each tissue were then pooled for RNA-Seq. cDNA library construction and sequencing RNA-Seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2013 | Rokyta, DR;Wray, KP;Margres, MJ; | The genesis of an exceptionally lethal venom in the timber rattlesnake (Crotalus horridus) revealed through comparative venom-gland transcriptomics | BMC Genomics | 23758969 | Sequencing and nonnormalized cDNA library preparation were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially as described by Mort | Full Paper |
2013 | Sugihara, K;Shibata, TK;Takata, K;Kimura, T;Kanayama, N;Williams, R;Hatakeyama, S;Akama, TO;Kuo, CW;Khoo, KH;Fukuda, MN; | Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycans | FEBS Lett. | 23968720 | PolyLacs were collected and desalted using a Sephadex G-25 column and lyophilized. 2.7. Immunohistochemistry. Paraffin-embedded sections of human testis, epididymis, and ductus deferens tissues were obtained from Folio Biosciences (Columbus, OH) | Full Paper |
2013 | Gurudatta, BV;Yang, J;Van Bortle, K;Donlin-Asp, PG;Corces, VG; | Dynamic changes in the genomic localization of DNA replication-related element binding factor during the cell cycle | Cell Cycle | 23624840 | The reaction was stopped by adding glycine to a final concentration of 0.125 mM. Chromatin was prepared by shearing the chromatin using sonication. Chromatin was immunoprecipitated using antibodies to DREF,35,40 and the DNA was used for library prepa | Full Paper |
2013 | Haugarvoll, K;Johansson, S;Tzoulis, C;Haukanes, BI;Bredrup, C;Neckelmann, G;Boman, H;Knappskog, PM;Bindoff, LA; | MRI characterisation of adult onset alpha-methylacyl-coA racemase deficiency diagnosed by exome sequencing | Orphanet J Rare Dis | 23286897 | Genealogical data from the patients, local parish registers and population censuses were used. Whole genome, single nucleotide polymorphism (SNP) genotyping was performed utilizing the Genome-Wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA) and | Full Paper |
2013 | O'Donnell, P;Ferguson, J;Shyu, J;Current, R;Rehage, T;Tsai, J;Christensen, M;Tran, HB;Chien, SS;Shieh, F;Wei, W;Lawrence, HJ;Wu, L;Schilling, R;Bloom, K;Maltzman, W;Anderson, S;Soviero, S; | Analytic performance studies and clinical reproducibility of a real-time PCR assay for the detection of epidermal growth factor receptor gene mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancer | BMC Cancer | 23621958 | (Wilmington, DE, USA), Asterand,Inc. (Detroit, MI, USA), BioServe (Beltsville, MD, USA), Conversant (Huntsville,AL, USA), Cureline Inc. (South San Francisco, CA, USA), Cytomyx (Lexington, MA,USA), Discovery Life Sciences, Inc | Full Paper |
2013 | Peatman, E;Li, C;Peterson, BC;Straus, DL;Farmer, BD;Beck, BH; | Basal polarization of the mucosal compartment in Flavobacterium columnare susceptible and resistant channel catfish (Ictalurus punctatus) | Mol. Immunol. | 23895942 | ; preserving the replicate pools for replicated QPCR validation. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25 | Full Paper |
2013 | Sookruksawong, S;Sun, F;Liu, Z;Tassanakajon, A; | RNA-Seq analysis reveals genes associated with resistance to Taura syndrome virus (TSV) in the Pacific white shrimp Litopenaeus vannamei | Dev. Comp. Immunol. | 23921257 | RNA-Seq library preparation and sequencing was outsourced and performed in the Genomic Services Lab of the HudsonAlpha Biotechnology Institute (Huntsville, AL, USA) as previously described (Li et al., 2012b; Sun et al., 2012). The cDNA libraries of T | Full Paper |
2013 | Macias, JD;Gerkin, RD;Locke, D;Macias, MP; | Differential gene expression in cholesteatoma by DNA chip analysis | Laryngoscope | 23670528 | From the Macias Otology (J.D.M.), Biomedical Research Program, the EAR Foundation of Arizona (J.D.M., M.P.M.), and Scientific Services, Banner Health Research Institute (R.D.G.), Phoenix, Arizona, USA; and QualTek Molecular Laboratories (D.L.), Newto | Full Paper |
2013 | Infante, CR;Park, S;Mihala, AG;Kingsley, DM;Menke, DB; | Pitx1 broadly associates with limb enhancers and is enriched on hindlimb cis-regulatory elements | Dev. Biol. | 23201014 | Two independent biological replicates were used to generate two Illumina ChIP-Seq and two control libraries. All ChIP and input chromatin control libraries were produced using the Illumina ChIP-Seq DNA Sample Prep Kit (IP-102-1001) as directed by the | Full Paper |
2013 | Tzoulis, C;Johansson, S;Haukanes, BI;Boman, H;Knappskog, PM;Bindoff, LA; | Novel SACS mutations identified by whole exome sequencing in a norwegian family with autosomal recessive spastic ataxia of Charlevoix-Saguenay | PLoS ONE | 23785480 | Whole exome sequencing was performed at HudsonAlpha Institute for Biotechnology (Huntsville,AL) using Roche-NimbleGen Sequence Capture EZ Exome v2 kit and paired-end 100nt sequencing on the Illumina HiSeq [21]. | Full Paper |
2013 | Xu, J;Ji, P;Wang, B;Zhao, L;Wang, J;Zhao, Z;Zhang, Y;Li, J;Xu, P;Sun, X; | Transcriptome sequencing and analysis of wild Amur Ide (Leuciscus waleckii) inhabiting an extreme alkaline-saline lake reveals insights into stress adaptation | PLoS ONE | 23573207 | ;... lity RNA samples from each tissue were then pooled for cDNA synthesis and sequencing. cDNA Library Construction, Sequencing and Assembly RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2013 | Jiang, Y;Ninwichian, P;Liu, S;Zhang, J;Kucuktas, H;Sun, F;Kaltenboeck, L;Sun, L;Bao, L;Liu, Z; | Generation of physical map contig-specific sequences useful for whole genome sequence scaffolding | PLoS ONE | 24205335 | One microliter ligation product was used as template for amplification with the following reaction cocktail: 1 µl 10X buffer, 1 U polymerase, 0.4 µl MgCl2 [50 mM], 0.8 µl dNTPs [2.5 mM], 2µl [5 µM] in-house-designed primers. Reactions were conducted | Full Paper |
2013 | Liu, L;Li, C;Su, B;Beck, BH;Peatman, E; | Short-term feed deprivation alters immune status of surface mucosa in channel catfish (Ictalurus punctatus) | PLoS ONE | 24023952 | ;... ach condition, equal amounts of RNA from skin and gill were pooled to generate three pooled replicates for RNA-seq library construction. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2013 | Sun, F;Liu, S;Gao, X;Jiang, Y;Perera, D;Wang, X;Li, C;Sun, L;Zhang, J;Kaltenboeck, L;Dunham, R;Liu, Z; | Male-biased genes in catfish as revealed by RNA-Seq analysis of the testis transcriptome | PLoS ONE | 23874634 | ;... Bioanalysis chip. Equal amount of total RNA from catfish testis of each age was pooled together into only one sample for use in RNA-Seq. RNA-Seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2013 | Garofalo, M;Jeon, YJ;Nuovo, GJ;Middleton, J; | MiR-34a/c-dependent PDGFR-α/β downregulation inhibits tumorigenesis and enhances TRAIL-induced apoptosis in lung cancer | PloS one | The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: GJN is an employee of Phylogeny, Inc. There are no patents, products in development or marketed product | Full Paper | |
2013 | Lopez-Rios, F;Angulo, B;Gomez, B;Mair, D;Martinez, R;Conde, E;Shieh, F;Tsai, J;Vaks, J;Current, R;Lawrence, HJ;Gonzalez de Castro, D; | Comparison of molecular testing methods for the detection of EGFR mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancer | J. Clin. Pathol. | 23386666 | Six FFPET specimens were purchased from INDIVUMED Inc. (Kensington, Maryland, USA), Cureline, Inc. (South San Francisco,California, USA), Bioserve (Beltsville, Maryland, USA), and ConversantBio (Huntsville, Alabama, USA). Study design | Full Paper |
2013 | Lamerz, J;Friedlein, A;Soder, N;Cutler, P;Döbeli, H; | Determination of free desmosine in human plasma and its application in two experimental medicine studies | Anal. Biochem. | 23399390 | To validate the method, we used a plasma sample collection containing 30 patients with diagnosed COPD and 30 donors without a COPD diagnosis. This collection was obtained from Conversant Bio (Huntsville, AL, USA). Apart | Full Paper |
2013 | Pawar, H;Maharudraiah, J;Kashyap, MK;Sharma, J; | Downregulation of cornulin in esophageal squamous cell carcinoma | Acta histochemica | The ESCCs varied from tumor grade I to grade III from patients in the age group of 36-78 years. A second TMA set was obtained from FolioBio (Catalog no. ARY-HH0091, Powell, OH, USA) consisting of 40 ESCC cases with matched adjacent normal esophageal | Full Paper | |
2013 | Adorno, M;Sikandar, S;Mitra, SS;Kuo, A;Nicolis Di Robilant, B;Haro-Acosta, V;Ouadah, Y;Quarta, M;Rodriguez, J;Qian, D;Reddy, VM;Cheshier, S;Garner, CC;Clarke, MF; | Usp16 contributes to somatic stem-cell defects in Down's syndrome | Nature | 24025767 | Control bone marrow samples were obtained from AllCells, LLC. All samples were Control bone marrow samples were obtained from AllCells, LLC. Although we cannot unequivocally Save | Full Paper |
2013 | Amir, el-AD;Davis, KL;Tadmor, MD;Simonds, EF;Levine, JH;Bendall, SC;Shenfeld, DK;Krishnaswamy, S;Nolan, GP;Pe'er, D; | viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemia | Nature biotechnology | 23685480 | Fresh, Ficoll-enriched human bone marrow was obtained from healthy donors from AllCells, Inc. (Emeryville, CA). Samples were obtained with informed consent in accordance with the Save | Full Paper |
2013 | Meng, X;Su, RJ;Baylink, DJ;Neises, A;Kiroyan, JB;Lee, WY;Payne, KJ;Gridley, DS;Wang, J;Lau, KH;Li, G;Zhang, XB; | Rapid and efficient reprogramming of human fetal and adult blood CD34+ cells into mesenchymal stem cells with a single factor | Cell research | 23478301 | University and written informed consent was obtained from all participants. CD34+ cells were purified with a CD34 Microbead Kit (Miltenyi Biotec, Auburn, CA, USA). Mobilized PB CD34+ cells were purchased from AllCells LLC (Emeryville, CA, USA) | Full Paper |
2013 | Yang, R;Liu, Y;Kelk, P;Qu, C;Akiyama, K;Chen, C;Atsuta, I;Chen, W;Zhou, Y;Shi, S; | A subset of IL-17(+) mesenchymal stem cells possesses anti-Candida albicans effect | Cell research | 23266891 | Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells (Emeryville, CA, USA). Human bone marrow progenitor cells were enriched using fresh human bone marrow by RosetteSep® Human Bone Marrow Progenitor | Full Paper |
2013 | Oshima, T;Sato, S;Kato, J;Ito, Y;Watanabe, T;Tsuji, I;Hori, A;Kurokawa, T;Kokubo, T; | Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers | Molecular cancer | 23758976 | Human peripheral blood mononuclear cells (PBMC) from AllCells were cultured in RPMI1640 medium containing 10% FBS, 0.1 nM human IL-2 (DIACLONE Research), and 55 μM 2- Save | Full Paper |
2013 | Takeishi, S;Matsumoto, A;Onoyama, I;Naka, K;Hirao, A;Nakayama, KI; | Ablation of Fbxw7 eliminates leukemia-initiating cells by preventing quiescence | Cancer cell | 23518349 | bone marrow mononuclear cells from treatment-naive patients in the chronic phase of CML and from healthy volunteers who had given informed consent were obtained from AllCells. Save | Full Paper |
2013 | Deckert, J;Park, PU;Chicklas, S;Yi, Y;Li, M;Lai, KC;Mayo, MF;Carrigan, CN;Erickson, HK;Pinkas, J;Lutz, RJ;Chittenden, T;Lambert, JM; | A novel anti-CD37 antibody-drug conjugate with multiple anti-tumor mechanisms for the treatment of B-cell malignancies | Blood | 24002446 | Frozen PBMCs from patients with CLL were obtained from AllCells (Emeryville, CA). For each sample, 5 × 10 5 cells were stained for indicated blood cell markers (Miltenyi) together with Save | Full Paper |
2013 | Cutler, C;Multani, P;Robbins, D;Kim, HT;Le, T;Hoggatt, J;Pelus, LM;Desponts, C;Chen, YB;Rezner, B;Armand, P;Koreth, J;Glotzbecker, B;Ho, VT;Alyea, E;Isom, M;Kao, G;Armant, M;Silberstein, L;Hu, P;Soiffer, RJ;Scadden, DT;Ritz, J;Goessling, W;North, TE;Mendlein, J;Ballen, K;Zon, LI;Antin, JH;Shoemaker, DD; | Prostaglandin-modulated umbilical cord blood hematopoietic stem cell transplantation | Blood | 23996087 | Primary cells Cryopreserved human UCB CD34+ primary cells were purchased from AllCells or Stem Cell Technologies (Vancouver, BC, Canada) | Full Paper |
2013 | Malinge, S;Chlon, T;Doré, LC;Ketterling, RP;Tallman, MS;Paietta, E;Gamis, AS;Taub, JW;Chou, ST;Weiss, MJ;Crispino, JD;Figueroa, ME; | Development of acute megakaryoblastic leukemia in Down syndrome is associated with sequential epigenetic changes | Blood | 23980066 | Michigan, and the University of Michigan. Eight specimens of human CD34+ bone marrow cells were provided by the Stem Cell and Xenograft Core Facility of the University of Pennsylvania or purchased from AllCells (Emeryville, CA). These studies were pe | Full Paper |
2013 | Kung Sutherland, MS;Walter, RB;Jeffrey, SC;Burke, PJ;Yu, C;Kostner, H;Stone, I;Ryan, MC;Sussman, D;Lyon, RP;Zeng, W;Harrington, KH;Klussman, K;Westendorf, L;Meyer, D;Bernstein, ID;Senter, PD;Benjamin, DR;Drachman, JG;McEarchern, JA; | SGN-CD33A: a novel CD33-targeting antibody-drug conjugate using a pyrrolobenzodiazepine dimer is active in models of drug-resistant AML | Blood | 23770776 | For the primary AML xenograft model, NOD/SCID/IL-2Rgnull mice (NSG; The Jackson Laboratory, Bar Harbor, ME) were irradiated with 1 Gy 1 day before intravenous injection of 7 × 105 primary leukemia cells from a patient with relapsed AML (06227; AllCel | Full Paper |
2013 | Kojima, K;Kornblau, SM;Ruvolo, V;Dilip, A;Duvvuri, S;Davis, RE;Zhang, M;Wang, Z;Coombes, KR;Zhang, N;Qiu, YH;Burks, JK;Kantarjian, H;Shacham, S;Kauffman, M;Andreeff, M; | Prognostic impact and targeting of CRM1 in acute myeloid leukemia | Blood | 23564911 | Normal marrow CD34 + cells from healthy donors aged 18 to 55 were obtained from AllCells (Emeryville, CA). The demographics and clinical characteristics of the patient population Save | Full Paper |
2013 | Lee, J;Shieh, JH;Zhang, J;Liu, L;Zhang, Y;Eom, JY;Morrone, G;Moore, MA;Zhou, P; | Improved ex vivo expansion of adult hematopoietic stem cells by overcoming CUL4-mediated degradation of HOXB4 | Blood | 23520338 | Each irradiated mouse (5 mice/group) was transplanted via tail vein injection with 3 × 105 CD34+ G-CSF-mobilized adult HSCs (AllCells, LLC, Emeryville, CA) treated with 2 rounds of purified recombinant wild-type or degradation-resistant HOXB4 protein | Full Paper |
2013 | Bhagat, TD;Zhou, L;Sokol, L;Kessel, R;Caceres, G;Gundabolu, K;Tamari, R;Gordon, S;Mantzaris, I;Jodlowski, T;Yu, Y;Jing, X;Polineni, R;Bhatia, K;Pellagatti, A;Boultwood, J;Kambhampati, S;Steidl, U;Stein, C;Ju, W;Liu, G;Kenny, P;List, A;Bitzer, M;Verma, A; | miR-21 mediates hematopoietic suppression in MDS by activating TGF-β signaling | Blood | 23390194 | Bone marrow CD34+ cells from various normal donors were also obtained from AllCells (Emeryville, CA). HS-5 cell line (CRL-11882) was purchased from ATCC (Manassas, VA). TGF-β1 was bought from R&D Systems (Minneapolis, MN). The phos-SMAD2 (S465/467) a | Full Paper |
2013 | Kumkhaek, C;Aerbajinai, W;Liu, W;Zhu, J;Uchida, N;Kurlander, R;Hsieh, MM;Tisdale, JF;Rodgers, GP; | MASL1 induces erythroid differentiation in human erythropoietin-dependent CD34+ cells through the Raf/MEK/ERK pathway | Blood | 23327923 | CD34+ cells were obtained from commercial sources (Lonza, Walkerville, MD, or AllCells, Emeryville, CA). Cells were thawed and washed into StemSpan serum-free expansion medium (SFEM) (StemCell Technologies, Vancouver, BC, Canada) and then seeded in S | Full Paper |
2013 | Wong, KY;Yim, RL;Kwong, YL;Leung, CY;Hui, PK;Cheung, F;Liang, R;Jin, DY;Chim, CS; | Epigenetic inactivation of the MIR129-2 in hematological malignancies | Journal of hematology & oncology | 23406679 | MIR124-1 and MIR203 were previously described [14,16]. Mononuclear cell DNA from 15 healthy donors [8 bone marrow, 3 peripheral blood, 3 CD19-sorted peripheral blood, and 1 CD138-sorted bone marrow (AllCells, CA, USA)] were used as negative control, | Full Paper |
2013 | Richardson, CL;Delehanty, LL;Bullock, GC;Rival, CM;Tung, KS;Kimpel, DL;Gardenghi, S;Rivella, S;Goldfarb, AN; | Isocitrate ameliorates anemia by suppressing the erythroid iron restriction response | The Journal of clinical investigation | 23863711 | The unique sensitivity of early red cell progenitors to iron deprivation, known as the erythroid iron restriction response, serves as a basis for human anemias globally. This response Save | Full Paper |
2013 | McBrian, MA;Behbahan, IS;Ferrari, R;Su, T;Huang, TW;Li, K;Hong, CS;Christofk, HR;Vogelauer, M;Seligson, DB;Kurdistani, SK; | Histone acetylation regulates intracellular pH | Molecular cell | 23201122 | H1 cells were maintained in mTeSR1 medium (STEMCELL Technologies #05850) on Matrigel. T cells were purchased from AllCells (PB009-1F) and were maintained in RPMI-1640 (ATCC #30-2001) with 10% FBS (Hyclone #SV3001403) that was heat inactivated by trea | Full Paper |
2013 | Kuroda, Y;Wakao, S;Kitada, M;Murakami, T;Nojima, M;Dezawa, M; | Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells | Nature protocols | 23787896 | For example, when we purchased bone marrow from ALLCELLS, which is imported from the United States to Japan and typically takes 5-7 d to arrive, the number of mononuclear cells Save | Full Paper |
2013 | Cheng, DT;Ma, C;Niewoehner, J;Dahl, M;Tsai, A;Zhang, J;Gonsiorek, W;Apparsundaram, S;Pashine, A;Ravindran, P;Jung, J;Hang, J;Allard, J;Bitter, H;Tribouley, C;Narula, S;Wilson, S;Fuentes, ME; | Thymic stromal lymphopoietin receptor blockade reduces allergic inflammation in a cynomolgus monkey model of asthma | The Journal of allergy and clinical immunology | 23810153 | Single donor human cord blood-derived CD34 + cells were purchased from AllCells (Emeryville, Calif). Cells were cultured for 8 weeks in serum-free complete medium (StemPro-34 Save | Full Paper |
2013 | Leone, E;Morelli, E;Di Martino, MT;Amodio, N;Foresta, U;Gullà, A;Rossi, M;Neri, A;Giordano, A;Munshi, NC;Anderson, KC;Tagliaferri, P;Tassone, P; | Targeting miR-21 inhibits in vitro and in vivo multiple myeloma cell growth | Clinical cancer research : an official journal of the American Association for Cancer Research | 23446999 | Imgenex) by standard procedures (37, 38). hBMSCs were obtained by long-term culture of bone marrow mononuclear cells (39). For coculture, 1 × 105 ppMM cells were seeded on 2 × 104 hBMSCs for 24 to 48 hours in 96-well plates. RNA samples of normal hea | Full Paper |
2013 | Kuhne, MR;Mulvey, T;Belanger, B;Chen, S;Pan, C;Chong, C;Cao, F;Niekro, W;Kempe, T;Henning, KA;Cohen, LJ;Korman, AJ;Cardarelli, PM; | BMS-936564/MDX-1338: a fully human anti-CXCR4 antibody induces apoptosis in vitro and shows antitumor activity in vivo in hematologic malignancies | Clinical cancer research : an official journal of the American Association for Cancer Research | 23213054 | Binding Buffer 10× concentrate, 7-aminoactinomycin D (7-AAD), and Annexin V-APC from BD Biosciences; phycoerythrin (PE)-conjugated goat anti-human antibody from Jackson ImmunoResearch (Cat. 109-116-098). AML peripheral blood mononuclear cells (PBMC) | Full Paper |
2013 | Corbin, AS;O'Hare, T;Gu, Z;Kraft, IL;Eiring, AM;Khorashad, JS;Pomicter, AD;Zhang, TY;Eide, CA;Manley, PW;Cortes, JE;Druker, BJ;Deininger, MW; | KIT signaling governs differential sensitivity of mature and primitive CML progenitors to tyrosine kinase inhibitors | Cancer research | 23887971 | Normal bone marrow mononuclear cells (MNC) were from AllCells. Cell selection was as described previously (details in Supplementary Methods; ref. 17). Save | Full Paper |
2013 | Ara, T;Nakata, R;Sheard, MA;Shimada, H;Buettner, R;Groshen, SG;Ji, L;Yu, H;Jove, R;Seeger, RC;DeClerck, YA; | Critical role of STAT3 in IL-6-mediated drug resistance in human neuroblastoma | Cancer research | 23633489 | AmpFISTR Identifier PCR kit and GeneMapper ID v. 3.2 (Applied Biosystems). Human BMMSC were purchased from AllCells LLC. Monocytes of normal healthy donors were obtained from peripheral blood and separated by Ficoll density gradient centrifugation us | Full Paper |
2013 | Wei, Y;Chen, R;Dimicoli, S;Bueso-Ramos, C;Neuberg, D;Pierce, S;Wang, H;Yang, H;Jia, Y;Zheng, H;Fang, Z;Nguyen, M;Ganan-Gomez, I;Ebert, B;Levine, R;Kantarjian, H;Garcia-Manero, G; | Global H3K4me3 genome mapping reveals alterations of innate immunity signaling and overexpression of JMJD3 in human myelodysplastic syndrome CD34+ cells | Leukemia | 23538751 | BMs from healthy individuals were obtained from AllCells (Emeryville, CA, USA). Isolation of CD34+ cells was performed using MicroBead Kit (Miltenyi, Bergisch Gladbach, Germany) Save | Full Paper |
2013 | Rio-Machin, A;Ferreira, BI;Henry, T;Gómez-López, G;Agirre, X;Alvarez, S;Rodriguez-Perales, S;Prosper, F;Calasanz, MJ;Martínez, J;Fonseca, R;Cigudosa, JC; | Downregulation of specific miRNAs in hyperdiploid multiple myeloma mimics the oncogenic effect of IgH translocations occurring in the non-hyperdiploid subtype | Leukemia | 23174883 | CD138-positive snap-frozen plasma cell pellets (ALLCELLS, Emeryville, CA, USA) were DNA from healthy CD138+ snap-frozen plasma cell pellets (ALLCELLS, Emeryville, CA, USA) Save | Full Paper |
2013 | Díaz-Beyá, M;Navarro, A;Ferrer, G;Díaz, T;Gel, B;Camós, M;Pratcorona, M;Torrebadell, M;Rozman, M;Colomer, D;Monzo, M;Esteve, J; | Acute myeloid leukemia with translocation (8;16)(p11;p13) and MYST3-CREBBP rearrangement harbors a distinctive microRNA signature targeting RET proto-oncogene | Leukemia | 23022987 | In addition, CD34+ cells from the bone marrow of three healthy donor subjects were purchased from AllCells (Emeryville, CA, USA). All patients provided their written informed consent Save | Full Paper |
2013 | Hayashi, Y;Hirai, H;Kamio, N;Yao, H;Yoshioka, S;Miura, Y;Ashihara, E;Fujiyama, Y;Tenen, DG;Maekawa, T; | C/EBPβ promotes BCR-ABL-mediated myeloid expansion and leukemic stem cell exhaustion | Leukemia | 22948537 | Frozen samples of human bone marrow cells from healthy donors or untreated CP-CML patients were purchased from AllCells LLC (Emeryville, CA, USA). In all cases, written informed Save;[方紘] 慢性期 CML 患者および健常人ドナーの骨髄細胞検体は AllCells 社より購入した. マウス 血幹細胞の細胞 で | Full Paper |
2013 | Lin, X;Rice, KL;Buzzai, M;Hexner, E;Costa, FF;Kilpivaara, O;Mullally, A;Soares, MB;Ebert, BL;Levine, R;Licht, JD; | miR-433 is aberrantly expressed in myeloproliferative neoplasms and suppresses hematopoietic cell growth and differentiation | Leukemia | 22864358 | Human CD34+ cells were obtained from adult bone marrow (AllCells, Emeryville, CA, USA) and cultured in StemSpan serum-free expansion media (SFEM; Stem Cell Technologies, Save | Full Paper |
2013 | Shin, JW;Spinler, KR;Swift, J;Chasis, JA;Mohandas, N;Discher, DE; | Lamins regulate cell trafficking and lineage maturation of adult human hematopoietic cells | Proceedings of the National Academy of Sciences of the United States of America | 24191023 | Commercially available primary human cells from marrow were obtained from either AllCells, Inc. (Emeryville, CA) or anonymous donors to the University of Pennsylvania Stem Cell Save | Full Paper |
2013 | Xie, J;Zhang, H;Yea, K;Lerner, RA; | Autocrine signaling based selection of combinatorial antibodies that transdifferentiate human stem cells | Proceedings of the National Academy of Sciences of the United States of America | 23613575 | Differentiation of CD34+ Cells in Culture. Fresh bone marrow CD34+ cells were purchased from Allcells (catalog no. ABM010). The purity is greater than 96% as determined by FACS. The cells were washed with SFEM (Stem Cell 09600) and kept in SFEM with | Full Paper |
2013 | Hong, KU;Li, QH;Guo, Y;Patton, NS;Moktar, A;Bhatnagar, A;Bolli, R; | A highly sensitive and accurate method to quantify absolute numbers of c-kit+ cardiac stem cells following transplantation in mice | Basic research in cardiology | 23549981 | Female human peripheral mononuclear cells (hPBMCs) were purchased from AllCells, LLC (Emeryville, CA). Aliquots of 105 hPBMCs (in 50 μl PBS) were stored at −20 °C until use. For isolation of genomic DNA, QIAamp DNA Mini Kit (Qiagen) was used accordin | Full Paper |
2013 | Ramakrishnan, P;Clark, PM;Mason, DE;Peters, EC;Hsieh-Wilson, LC;Baltimore, D; | Activation of the transcriptional function of the NF-κB protein c-Rel by O-GlcNAc glycosylation | Science signaling | 23982206 | Biotechnology. The anti-FLAG antibody was from Sigma. The anti-O-GlcNAc antibodies RL2 and CTD 110.6 were from Abcam and Covance, respectively. Cells Jurkat, Jurkat T-REx, Ramos, EL4, MEFs, and HEK 293T cells were grown as previously described (23). | Full Paper |
2013 | Isgro, J;Gupta, S;Jacek, E;Pavri, T;Duculan, R;Kim, M;Kirou, KA;Salmon, JE;Pernis, AB; | Enhanced rho-associated protein kinase activation in patients with systemic lupus erythematosus | Arthritis and rheumatism | 23508371 | Cryopreserved vials of human cord blood CD4+ T lymphocytes were obtained from Lonza Poietics, Immune Cell Systems, and AllCells, LLC. CD4+ T cell purity was 95%. Cells were thawed according to manufacturer’s instructions and cultured in LGM-3 (Lonza) | Full Paper |
2013 | Allakhverdi, Z;Comeau, MR;Armant, M;Agrawal, R;Woodfolk, JA;Sehmi, R;Howie, KJ;Gauvreau, GM;Delespesse, G; | Mast Cell-Activated Bone Marrow Mesenchymal Stromal Cells Regulate Proliferation and Lineage Commitment of CD34(+) Progenitor Cells | Frontiers in immunology | 24381572 | ibody were used at 10 μg/ml, as in our previous study (4). Primary human bone marrow-derived mesenchymal stromal cells Human BM-derived mesenchymal stromal cells were established from BM samples (AllCells, Emeryville, CA, USA) by culture in minimum | Full Paper |
2013 | Wu, Q;Wang, H;Zhao, X;Shi, Y;Jin, M;Wan, B;Xu, H;Cheng, Y;Ge, H;Zhang, Y; | Identification of G-protein-coupled receptor 120 as a tumor-promoting receptor that induces angiogenesis and migration in human colorectal carcinoma | Oncogene | 23851494 | ECMatrix (50 μl, Merck KGaA ) was distributed per well in a 96-well plate. HUVECs (ALLCELLS, Shanghai, China, 2 × 104 cells/well) suspended in 100 μl of medium supplemented with 0.5% FBS were added to each well and cultured in triplicate with 150 μl | Full Paper |
2013 | Li, C;Takahashi, C;Zhang, L;Huseni, M;Stankovich, B;Mashhedi, H;Lee, J;French, D;Anderson, JE;Kim, D;Howell, K;Brauer, MJ;Kowanetz, M;Yan, Y;Humke, E;Ebens, A;Hampton, G;Lackner, MR;Hegde, P;Jia, S; | Development of a robust flow cytometry-based pharmacodynamic assay to detect phospho-protein signals for phosphatidylinositol 3-kinase inhibitors in multiple myeloma | Journal of translational medicine | 23522020 | le myeloma Li Congfen 1 li.congfen@gene.com Takahashi Chikara 1 takahashi.chikara@gene.com Zhang Liangxuan 1 zhang.liangxuan@gene.com Huseni Mahrukh 1 mahrukhh@gene.com Stankovich Basha 2 bstankovich@allcells.com Mashhedi Haider 1 mashhedh@gene.com L | Full Paper |
2013 | Nazarov, C;Lo Surdo, J;Bauer, SR;Wei, CH; | Assessment of immunosuppressive activity of human mesenchymal stem cells using murine antigen specific CD4 and CD8 T cells in vitro | Stem cell research & therapy | 24406271 | by the Association for Assessment and Accreditation of Laboratory Animal Care International. All experiments were performed according to institutional guidelines. Cell culture hMSCs were purchased (AllCells, Emeryville, CA, USA) at passage one. Acco | Full Paper |
2013 | Ledford, KJ;Zeigler, F;Bartel, RL; | Ixmyelocel-T, an expanded multicellular therapy, contains a unique population of M2-like macrophages | Stem cell research & therapy | 24405629 | For the generation of M1 and M2 macrophages, human peripheral blood monocytes from healthy donors were purchased (AllCells LLC., Alameda, USA). The cells were polarized into Save | Full Paper |
2013 | Vanella, L;Sodhi, K;Kim, DH;Puri, N;Maheshwari, M;Hinds, TD;Bellner, L;Goldstein, D;Peterson, SJ;Shapiro, JI;Abraham, NG; | Increased heme-oxygenase 1 expression in mesenchymal stem cell-derived adipocytes decreases differentiation and lipid accumulation via upregulation of the canonical Wnt signaling cascade | Stem cell research & therapy | 23497794 | Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA, USA). After thawing, mononuclear cells were resuspended in an α-minimal essential Save | Full Paper |
2013 | McNamara, LA;Onafuwa-Nuga, A;Sebastian, NT;Riddell, J;Bixby, D;Collins, KL; | CD133+ hematopoietic progenitor cells harbor HIV genomes in a subset of optimally treated people with long-term viral suppression | The Journal of infectious diseases | 23554378 | and 7-aminoactinomycin D (7-AAD). Healthy donor BMMCs (AllCells) were stained with R-phycoerythrin-conjugated anti-CD133, R-phycoerythrin-Cy7-conjugated anti-CD34 (BD), and 7-AAD. Samples were analyzed on a BD FACSCanto | Full Paper |
2013 | Moy, LY;Jia, Y;Caniga, M;Lieber, G;Gil, M;Fernandez, X;Sirkowski, E;Miller, R;Alexander, JP;Lee, HH;Shin, JD;Ellis, JM;Chen, H;Wilhelm, A;Yu, H;Vincent, S;Chapman, RW;Kelly, N;Hickey, E;Abraham, WM;Northrup, A;Miller, T;Houshyar, H;Crackower, MA; | Inhibition of spleen tyrosine kinase attenuates allergen-mediated airway constriction | American journal of respiratory cell and molecular biology | 23889698 | + cells (5 x 10 6 /vial, AllCells) were thawed and added into 50 ml polypropylene conical tube with 100 µL of 1mg/ml DNase I (StemCell). 15.5 ml of pre-warmed Dulbecco's modified Save | Full Paper |
2013 | Ye, L;Muench, MO;Fusaki, N;Beyer, AI;Wang, J;Qi, Z;Yu, J;Kan, YW; | Blood cell-derived induced pluripotent stem cells free of reprogramming factors generated by Sendai viral vectors | Stem cells translational medicine | 23847002 | Frozen human peripheral blood mobilized CD34+ cells were purchased from AllCells (Emeryville, CA, http://www.allcells.com). After thawing, CD34+ cells were cultured with serum-free medium (SFM) as described by Ye and coworkers [24, 29], containing 50 | Full Paper |
2013 | Hall, SR;Jiang, Y;Leary, E;Yavanian, G;Eminli, S;O'Neill, DW;Marasco, WA; | Identification and isolation of small CD44-negative mesenchymal stem/progenitor cells from human bone marrow using elutriation and polychromatic flow cytometry | Stem cells translational medicine | 23847000 | Fresh, mobilized leukapheresis products were purchased from AllCells (Emeryville, CA, http://www.allcells.com) or collected from healthy volunteers at NeoStem Laboratory (Cambridge, MA, http://www.neostem.com) under an institutional review board-appr | Full Paper |
2013 | Mendelson, A;Cheung, Yk;Paluch, K;Chen, M;Kong, K;Tan, J;Dong, Z;Sia, SK;Mao, JJ; | Competitive stem cell recruitment by multiple cytotactic cues | Lab on a chip | 23364311 | Human bone marrow MSCs were isolated from fresh adult donor samples (AllCells, Emeryville, CA) using our prior methods. Briefly, the bone marrow sample was incubated with the Save | Full Paper |
2013 | Cairo, C;Sagnia, B;Cappelli, G;Colizzi, V;Leke, RG;Leke, RJ;Pauza, CD; | Human cord blood γδ T cells expressing public Vγ2 chains dominate the response to bisphosphonate plus interleukin-15 | Immunology | 23181340 | fraction of CBMC was reserved for cell culture and 0·5 × 107 to 1 × 107 were lysed for RNA extraction; remaining cells were frozen at 1 × 107 CBMC/ml, in 90% fetal bovine serum (FBS), 10% DMSO freezing medium. Some CBMC specimens were purchased from | Full Paper |
2013 | Kim, DH;Puri, N;Sodhi, K;Falck, JR;Abraham, NG;Shapiro, J;Schwartzman, ML; | Cyclooxygenase-2 dependent metabolism of 20-HETE increases adiposity and adipocyte enlargement in mesenchymal stem cell-derived adipocytes | Journal of lipid research | 23293373 | Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α-MEM, Save;ates: hypertension, the metabolic syndrome, and diabetes. MATERIALS | Full Paper |
2013 | Luo, Y;Kleiboeker, S;Deng, X;Qiu, J; | Human parvovirus B19 infection causes cell cycle arrest of human erythroid progenitors at late S phase that favors viral DNA replication | Journal of virology | 24049177 | Cells and virus. (i) CD36+ EPCs. Human bone marrow CD34+ hematopoietic stem/progenitor cells (HSCs) were positively isolated using a direct immunomagnetic CD34+ MicroBead labeling system and were purchased from AllCells, LLC (Alameda, CA; catalog no | Full Paper |
2013 | Shen, Y;Zeng, L;Zhu, A;Blanc, T;Patel, D;Pennello, A;Bari, A;Ng, S;Persaud, K;Kang, YK;Balderes, P;Surguladze, D;Hindi, S;Zhou, Q;Ludwig, DL;Snavely, M; | Removal of a C-terminal serine residue proximal to the inter-chain disulfide bond of a human IgG1 lambda light chain mediates enhanced antibody stability and antibody dependent cell-mediated cytotoxicity | mAbs | 23567210 | drogenase (LDH) activity from the lysed targeted cells (A431 transfected with receptor target X, A431x) was monitored in the presence freshly isolated normal peripheral blood mononuclear cells (PBMC, AllCells, PB002) upon the treatment with λ or λdS | Full Paper |
2013 | Zhao, Y;Wang, J;Wang, M;Sun, P;Chen, J;Jin, X;Zhang, H; | Activation of bone marrow-derived mesenchymal stromal cells-a new mechanism of defocused low-energy shock wave in regenerative medicine | Cytotherapy | 24199590 | Human umbilical vein endothelial cells (HUVEC) were purchased from Allcells (Shanghai, China) and maintained in EGM2 medium (CC-3162, Lonza, Basel, Switzerland). HUVEC Save | Full Paper |
2013 | Jones, M;Varella-Garcia, M;Skokan, M;Bryce, S;Schowinsky, J;Peters, R;Vang, B;Brecheisen, M;Startz, T;Frank, N;Nankervis, B; | Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum System | Cytotherapy | 23992670 | Initially, six human bone marrow aspirates supplied by normal adult donors, who gave informed consent through a certified clinical collection facility (AllCells, Alameda, CA, USA), were Save | Full Paper |
2013 | Glen, KE;Workman, VL;Ahmed, F;Ratcliffe, E;Stacey, AJ;Thomas, RJ; | Production of erythrocytes from directly isolated or Delta1 Notch ligand expanded CD34+ hematopoietic progenitor cells: process characterization, monitoring and implications for manufacture | Cytotherapy | 23831361 | Background aims Economic ex vivo manufacture of erythrocytes at 10 12 cell doses requires an efficiently controlled bio-process capable of extensive proliferation and high terminal Save | Full Paper |
2013 | Song, L;Kauss, MA;Kopin, E;Chandra, M;Ul-Hasan, T;Miller, E;Jayandharan, GR;Rivers, AE;Aslanidi, GV;Ling, C;Li, B;Ma, W;Li, X;Andino, LM;Zhong, L;Tarantal, AF;Yoder, MC;Wong, KK;Tan, M;Chatterjee, S;Srivastava, A; | Optimizing the transduction efficiency of capsid-modified AAV6 serotype vectors in primary human hematopoietic stem cells in vitro and in a xenograft mouse model in vivo | Cytotherapy | 23830234 | CD34+ and umbilical cord blood CD34+ cells were either purchased from AllCells or obtained under a City of Hope Institutional Review Board approved protocol isolated as described by Chatterjee et al. [7], and maintained in either StemSpan SFEM medium | Full Paper |
2013 | Blackwood, E;Epler, J;Yen, I;Flagella, M;O'Brien, T;Evangelista, M;Schmidt, S;Xiao, Y;Choi, J;Kowanetz, K;Ramiscal, J;Wong, K;Jakubiak, D;Yee, S;Cain, G;Gazzard, L;Williams, K;Halladay, J;Jackson, PK;Malek, S; | Combination drug scheduling defines a "window of opportunity" for chemopotentiation of gemcitabine by an orally bioavailable, selective ChK1 inhibitor, GNE-900 | Molecular cancer therapeutics | 23873850 | For the bone marrow assay, fresh human bone marrow-derived cells were obtained from AllCells and plated at a density of 1 million cells/mL in Human Methylcellulose Complete Save | Full Paper |
2013 | Jekle, A;Yoon, J;Zuck, M;Najafi, R;Wang, L;Shiau, T;Francavilla, C;Rani, SA;Eitzinger, C;Nagl, M;Anderson, M;Debabov, D; | NVC-422 inactivates Staphylococcus aureus toxins | Antimicrobial agents and chemotherapy | 23208720 | Normal human peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA) and maintained in RPMI 1640 medium supplemented with 10% fetal bovine Save | Full Paper |
2013 | Winkler, DG;Faia, KL;DiNitto, JP;Ali, JA;White, KF;Brophy, EE;Pink, MM;Proctor, JL;Lussier, J;Martin, CM;Hoyt, JG;Tillotson, B;Murphy, EL;Lim, AR;Thomas, BD;Macdougall, JR;Ren, P;Liu, Y;Li, LS;Jessen, KA;Fritz, CC;Dunbar, JL;Porter, JR;Rommel, C;Palombella, VJ;Changelian, PS;Kutok, JL; | PI3K-δ and PI3K-γ inhibition by IPI-145 abrogates immune responses and suppresses activity in autoimmune and inflammatory disease models | Chemistry & biology | 24211136 | Primary Human B and T Cell Proliferation Assays Human peripheral blood CD19+ B cells and CD3+ T cells were purchased from Allcells and proliferation assays were conducted as described in Supplemental Experimental Procedures | Full Paper |
2013 | Liao, C;Hsu, J;Kim, Y;Hu, DQ;Xu, D;Zhang, J;Pashine, A;Menke, J;Whittard, T;Romero, N;Truitt, T;Slade, M;Lukacs, C;Hermann, J;Zhou, M;Lucas, M;Narula, S;DeMartino, J;Tan, SL; | Selective inhibition of spleen tyrosine kinase (SYK) with a novel orally bioavailable small molecule inhibitor, RO9021, impinges on various innate and adaptive immune responses: implications for SYK inhibitors in autoimmune disease therapy | Arthritis research & therapy | 24286216 | y kits (BD Biosciences). IgE-induced histamine release in human mast cells The method has been reported previously by our group [9]. Briefly, human cord blood-derived CD34+ hematopoietic stem cells (AllCells, Emeryville, CA, USA) were cultured in a | Full Paper |
2013 | Tsai, HC;Wu, R; | Cholera toxin directly enhances IL-17A production from human CD4+ T cells | Journal of immunology (Baltimore, Md. : 1950) | 24043897 | PBMCs were purchased from AllCells. According to the manufacturers' protocols, total CD4 + T cells were positively selected from PBMCs using magnetic CD4 MicroBeads (Miltenyi Save | Full Paper |
2013 | Sohn, SJ;Barrett, K;Van Abbema, A;Chang, C;Kohli, PB;Kanda, H;Smith, J;Lai, Y;Zhou, A;Zhang, B;Yang, W;Williams, K;Macleod, C;Hurley, CA;Kulagowski, JJ;Lewin-Koh, N;Dengler, HS;Johnson, AR;Ghilardi, N;Zak, M;Liang, J;Blair, WS;Magnuson, S;Wu, LC; | A restricted role for TYK2 catalytic activity in human cytokine responses revealed by novel TYK2-selective inhibitors | Journal of immunology (Baltimore, Md. : 1950) | 23894201 | IL-23 assay. IL-23 was obtained from R&D Systems and was used at a concentration that generated ∼50-90% of the maximal IL-23-induced signal in the assay (i.e., EC50-90 of IL-23). CD4 CD45RO+ T cells obtained from AllCells (Emeryville, CA) were cultur | Full Paper |
2013 | Yokley, BH;Selby, ST;Posch, PE; | A stimulation-dependent alternate core promoter links lymphotoxin α expression with TGF-β1 and fibroblast growth factor-7 signaling in primary human T cells | Journal of immunology (Baltimore, Md. : 1950) | 23547113 | Human T cells were purchased (Allcells, Emeryville, CA, USA) or were purified from PBMCs isolated from fresh whole blood with lymphocyte separation medium (Ficoll® and hypaque; Mediatech Inc, Manassas, VA, USA). Briefly, whole blood was brought to ro | Full Paper |
2013 | Fitzgerald, DC;Fonseca-Kelly, Z;Cullimore, ML;Safabakhsh, P;Saris, CJ;Zhang, GX;Rostami, A; | Independent and interdependent immunoregulatory effects of IL-27, IFN-β, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitis | Journal of immunology (Baltimore, Md. : 1950) | 23455508 | PBMCs were isolated using Ficoll-Paque Plus density centrifugation; cord blood cells were obtained from AllCells. CD4 + and CD14 + cells were purified by immunomagnetic Save | Full Paper |
2013 | Heuck, CJ;Mehta, J;Bhagat, T;Gundabolu, K;Yu, Y;Khan, S;Chrysofakis, G;Schinke, C;Tariman, J;Vickrey, E;Pulliam, N;Nischal, S;Zhou, L;Bhattacharyya, S;Meagher, R;Hu, C;Maqbool, S;Suzuki, M;Parekh, S;Reu, F;Steidl, U;Greally, J;Verma, A;Singhal, SB; | Myeloma is characterized by stage-specific alterations in DNA methylation that occur early during myelomagenesis | Journal of immunology (Baltimore, Md. : 1950) | 23408834 | Eight samples from normal donors without known malignancies were obtained commercially from AllCells (Emeryville, CA). All normal donor samples had a purity of ≥85%. Save | Full Paper |
2013 | Jiang, Z;Hu, X;Yu, H;Xu, Y;Wang, L;Chen, H;Chen, H;Wu, R;Zhang, Z;Xiang, C;Webster, KA;Wang, JA; | Human endometrial stem cells confer enhanced myocardial salvage and regeneration by paracrine mechanisms | Journal of cellular and molecular medicine | 23837896 | the supplemental information. Cell sources Human BMMSCs from three donors were purchased from Cyagen Bioscience (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Human EnSCs from three | Full Paper |
2013 | He, X;Xu, Z;Wang, B;Zheng, Y;Gong, W;Huang, G;Zhang, L;Li, Y;He, F; | Upregulation of thrombomodulin expression by activation of farnesoid X receptor in vascular endothelial cells | European journal of pharmacology | 24041925 | The primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells, LLC (Shanghai, China) and were cultured in the medium supplied by AllCells with 10% Save | Full Paper |
2013 | Larusch, GA;Merkulova, A;Mahdi, F;Shariat-Madar, Z;Sitrin, RG;Cines, DB;Schmaier, AH; | Domain 2 of uPAR regulates single-chain urokinase-mediated angiogenesis through β1-integrin and VEGFR2 | American journal of physiology. Heart and circulatory physiology | 23709605 | HUVECs were cultured in 96-well microtiter plates on gelatin at 1 × 10 4 cells/well for 24 h in EGM from AllCells. Cells were then washed with serum-free medium without growth factor Save | Full Paper |
2013 | Chen, Y;Chitapanarux, T;Wu, J;Soon, RK;Melton, AC;Yee, HF; | Inducible NOS mediates CNP-induced relaxation of intestinal myofibroblasts | American journal of physiology. Gastrointestinal and liver physiology | 23348803 | NPR Western blots were performed by AllCells (Emeryville, CA) with the following antibodies: anti-mouse NPR-A (clone ab116680; Abcam, Cambridge, MA), anti-mouse NPR-B (clone Save | Full Paper |
2013 | Koestler, DC;Christensen, B;Karagas, MR;Marsit, CJ;Langevin, SM;Kelsey, KT;Wiencke, JK;Houseman, EA; | Blood-based profiles of DNA methylation predict the underlying distribution of cell types: a validation analysis | Epigenetics | 23903776 | As previously described,10,18 our reference set (S0) consisted of sorted, normal, human, peripheral blood leukocyte subtypes purchased from AllCells. Leukocytes were isolated from different, anonymous, non-diseased individuals’ whole-blood by magneti | Full Paper |
2013 | Harb, W;Fan, A;Tran, T;Danila, DC;Keys, D;Schwartz, M;Ionescu-Zanetti, C; | Mutational Analysis of Circulating Tumor Cells Using a Novel Microfluidic Collection Device and qPCR Assay | Translational oncology | 24151533 | Healthy donor peripheral blood was obtained from a commercial supplier (AllCells, Emeryville, CA) under Institutional Review Board (IRB) consent. Blood was drawn into EDTA tubes ( Save | Full Paper |
2013 | Shahaduzzaman, M;Golden, JE;Green, S;Gronda, AE;Adrien, E;Ahmed, A;Sanberg, PR;Bickford, PC;Gemma, C;Willing, AE; | A single administration of human umbilical cord blood T cells produces long-lasting effects in the aging hippocampus | Age (Dordrecht, Netherlands) | 23263793 | Cryopreserved HUCB MNC fraction and its component subfractions (CD3+, CD4+, CD8+, CD14+, and CD133+) were obtained from AllCells, LLC. HUCB cells were thawed in a 37 C Save | Full Paper |
2013 | de Lichtervelde, L;Boitano, AE;Wang, Y;Krastel, P;Petersen, F;Cooke, MP;Schultz, PG; | Eupalinilide E inhibits erythropoiesis and promotes the expansion of hematopoietic progenitor cells | ACS chemical biology | 23441826 | Human mPB CD34 + cells were purified from fresh human leukophoresed mobilized peripheral blood (AllCells) using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following Save | Full Paper |
2013 | Xiao, L;Hung, KC;Takahashi, TT;Joo, KI;Lim, M;Roberts, RW;Wang, P; | Antibody-mimetic ligand selected by mRNA display targets DC-SIGN for dendritic cell-directed antigen delivery | ACS chemical biology | 23427768 | Human PBMC-derived DCs were generated and purified as previously described, (46, 47) except that human PBMCs were from HLA-A2+ patients (AllCells, LLC.). Briefly, plastic-adherent cell fractions of human PBMCs were grown in RPMI 1640 with 10% heat-in | Full Paper |
2013 | Čokić, VP;Smith, RD;Biancotto, A;Noguchi, CT;Puri, RK;Schechter, AN; | Globin gene expression in correlation with G protein-related genes during erythroid differentiation | BMC genomics | 23425329 | isolation system (Miltenyi Biotec, Auburn, CA). Commercial FL- (Cambrex Bio Science, Inc., Walkersville, MD), CB-, BM- and granulocyte-colony stimulating factor (G-CSF) stimulated mobilized PB- (mPB, AllCells LLC, Berkeley, CA) derived CD34+ cells ar | Full Paper |
2013 | Musiychuk, K;Sivalenka, R;Jaje, J;Bi, H;Flores, R;Shaw, B;Jones, RM;Golovina, T;Schnipper, J;Khandker, L;Sun, R;Li, C;Kang, L;Voskinarian-Berse, V;Zhang, X;Streatfield, S;Hambor, J;Abbot, S;Yusibov, V; | Plant-produced human recombinant erythropoietic growth factors support erythroid differentiation in vitro | Stem cells and development | 23517237 | Umbilical cord blood-derived CD34 + cells were obtained from AllCells and maintained in the StemSpan Serum-Free Expansion Medium (StemCell Technologies) containing 1 μM Save | Full Paper |
2013 | Ramalingam, S;London, V;Kandavelou, K;Cebotaru, L;Guggino, W;Civin, C;Chandrasegaran, S; | Generation and genetic engineering of human induced pluripotent stem cells using designed zinc finger nucleases | Stem cells and development | 22931452 | Human primary CBMNCs were purchased from Allcells, Inc. The frozen CBMNCs were thawed and then cultured in the serum free medium (SFM) supplemented with cytokines and a Save | Full Paper |
2013 | Chen, J;Shi, ZD;Ji, X;Morales, J;Zhang, J;Kaur, N;Wang, S; | Enhanced osteogenesis of human mesenchymal stem cells by periodic heat shock in self-assembling peptide hydrogel | Tissue engineering. Part A | 23072422 | Human bone marrow (BM) from the iliac crest of a 27-year-old donor was purchased (AllCells LLC, Berkeley, CA). Mononuclear cells were enriched and retrieved with RosetteSep MSC enrichment cocktail (StemCell Technologies, Vancouver, Canada) according | Full Paper |
2013 | Wei, N;Yu, Y;Joshi, V;Schmidt, T;Qian, F;Salem, AK;Stanford, C;Hong, L; | Glucocorticoid receptor antagonist and siRNA prevent senescence of human bone marrow mesenchymal stromal cells in vitro | Cell and tissue research | 23963647 | Fresh human bone marrows, donated by total six healthy male and female donors from 22 to 43 years of age, were purchased from Allcells (Emeryville, CA). Mononuclear cells were isolated using density gradient centrifugation (Histopaque-1.077; Sigma, S | Full Paper |
2013 | Hutchinson, AT;Malik, A;Berkahn, MB;Agostino, M;To, J;Tacchi, JL;Djordjevic, SP;Turnbull, L;Whitchurch, CB;Edmundson, AB;Jones, DR;Raison, RL;Ramsland, PA; | Formation of assemblies on cell membranes by secreted proteins: molecular studies of free λ light chain aggregates found on the surface of myeloma cells | The Biochemical journal | 23822104 | We have described the presence of cell-membrane-associated κFLCs (free immunoglobulin light chains) on the surface of myeloma cells. Notably, the anti-κFLC mAb (monoclonal Save | Full Paper |
2013 | Choudhary, R;Baturin, D;Fosmire, S;Freed, B;Porter, CC; | Knockdown of HPRT for selection of genetically modified human hematopoietic progenitor cells | PloS one | 23555045 | Human umbilical cord blood samples unsuitable for clinical use were obtained from the ClinImmune Labs or were purchased from AllCells (Emeryville, CA). CD34 + cells were isolated Save | Full Paper |
2013 | Song, L;Li, X;Jayandharan, GR;Wang, Y;Aslanidi, GV;Ling, C;Zhong, L;Gao, G;Yoder, MC;Ling, C;Tan, M;Srivastava, A; | High-efficiency transduction of primary human hematopoietic stem cells and erythroid lineage-restricted expression by optimized AAV6 serotype vectors in vitro and in a murine xenograft model in vivo | PloS one | 23516552 | fetal bovine serum (FBS; Sigma, St. Louis, MO), 100 µg/ml of penicillin and 100 U/ml of streptomycin (Invitrogen, Grand Island, NY). Human cord blood CD34+ cells and CD36+ cells were purchased from AllCells (AllCells Technologies, Emeryville, CA) and | Full Paper |
2013 | Wang, T;Choi, E;Monaco, MC;Campanac, E;Medynets, M;Do, T;Rao, P;Johnson, KR;Elkahloun, AG;Von Geldern, G;Johnson, T;Subramaniam, S;Hoffman, DA;Hoffman, D;Major, E;Nath, A; | Derivation of neural stem cells from human adult peripheral CD34+ cells for an autologous model of neuroinflammation | PloS one | 24303066 | marker to detect mesenchymal cells, as CD29 and CD271, were used to stain the isolated CD34+ to further asses their purity. CD34+ cell culture and transfection Cord blood CD34+ cells (purchased from Allcells, Emeryville, California) or adult CD34+ c | Full Paper |
2013 | Xiao, F;Kim, YC;Wen, H;Luo, J;Chen, P;Cowan, K;Wang, SM; | The genome of polymorphonuclear neutrophils maintains normal coding sequences | PloS one | 24250807 | loid differentiation. Our study concludes that genomic DNA from myeloid lineage cells can be safely used in genetic studies. Methods Ethics Statement: The cells used for the study were obtained from AllCells LLC (http://www.allcells.com/, Emeryville | Full Paper |
2013 | Kornblau, SM;Qutub, A;Yao, H;York, H;Qiu, YH;Graber, D;Ravandi, F;Cortes, J;Andreeff, M;Zhang, N;Coombes, KR; | Proteomic profiling identifies distinct protein patterns in acute myelogenous leukemia CD34+CD38- stem-like cells | PloS one | 24223100 | tcome. This population lack samples from the 10-15% of AML cases that are CD34 negative. As normal controls, 1x106 CD34+ cells were collected from 10 different unstimulated normal bone marrow donors (AllCells, Emeryville, CA http://www.allcells.com/) | Full Paper |
2013 | Chai, ZT;Kong, J;Zhu, XD;Zhang, YY;Lu, L;Zhou, JM;Wang, LR;Zhang, KZ;Zhang, QB;Ao, JY;Wang, M;Wu, WZ;Wang, L;Tang, ZY;Sun, HC; | MicroRNA-26a inhibits angiogenesis by down-regulating VEGFA through the PIK3C2α/Akt/HIF-1α pathway in hepatocellular carcinoma | PloS one | 24194905 | ecco’s modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA) containing 10% fetal bovine serum (FBS) in a humidified incubator at 37°C with an atmosphere of 5% CO2. HUVECs were purchased from Allcells (Shanghai, China) and cultured in Endothelial | Full Paper |
2013 | Tarantal, AF;Lee, CC;Kukis, DL;Cherry, SR; | Radiolabeling human peripheral blood stem cells for positron emission tomography (PET) imaging in young rhesus monkeys | PloS one | 24098579 | CD34+ hPBSC were obtained from a commercial source (AllCells, Berkeley, CA) and cryopreserved using a controlled rate cryopreservation protocol in aliquots as previously reported [ Save | Full Paper |
2013 | DeBoever, C;Reid, EG;Smith, EN;Wang, X;Dumaop, W;Harismendy, O;Carson, D;Richman, D;Masliah, E;Frazer, KA; | Whole transcriptome sequencing enables discovery and analysis of viruses in archived primary central nervous system lymphomas | PloS one | 24023918 | To serve as a positive control a cord blood sample was purchased from AllCells (Emeryville, CA). Peripheral blood mononuclear cells (PBMC) were extracted from peripheral blood Save | Full Paper |
2013 | Dimicoli, S;Wei, Y;Bueso-Ramos, C;Yang, H;Dinardo, C;Jia, Y;Zheng, H;Fang, Z;Nguyen, M;Pierce, S;Chen, R;Wang, H;Wu, C;Garcia-Manero, G; | Overexpression of the toll-like receptor (TLR) signaling adaptor MYD88, but lack of genetic mutation, in myelodysplastic syndromes | PloS one | 23976989 | Bone marrows from healthy individuals were obtained from AllCells (Emeryville, CA). CD34+ cells were isolated from fresh bone marrow specimens of patients with MDS using Save | Full Paper |
2013 | Dolley-Sonneville, PJ;Romeo, LE;Melkoumian, ZK; | Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditions | PloS one | 23940553 | Prior to the initiation of the multi-passage study we evaluated several plating densities based on recommendation of the manufacturer of hBM-MSCs used in the study (AllCells) and Save | Full Paper |
2013 | Holmberg, RC;Gindlesperger, A;Stokes, T;Lopez, D;Hyman, L;Freed, M;Belgrader, P;Harvey, J;Li, Z; | Akonni TruTip(®) and Qiagen(®) methods for extraction of fetal circulating DNA--evaluation by real-time and digital PCR | PloS one | 23936545 | ’s Circulating Nucleic Acid Kit. In order to determine the comparative extraction efficiency of the two methods on a large set of male fetal specimens, 19 maternal plasma samples were collected by AllCells (Emeryville, CA) or StemExpress (Placervill | Full Paper |
2013 | Hsiao, SC;Liu, H;Holstlaw, TA;Liu, C;Francis, CY;Francis, MB; | Real time assays for quantifying cytotoxicity with single cell resolution | PloS one | 23826123 | To each well were added 180 µL of freshly drawn human blood (AllCells, Inc.). After 16 h of incubation at 37C, the samples were visualized directly. Because the target cells are all Save | Full Paper |
2013 | Zhang, C;Wu, X;Zhang, M;Zhu, L;Zhao, R;Xu, D;Lin, Z;Liang, C;Chen, T;Chen, L;Ren, Y;Zhang, J;Qin, N;Zhang, X; | Small molecule R1498 as a well-tolerated and orally active kinase inhibitor for hepatocellular carcinoma and gastric cancer treatment via targeting angiogenesis and mitosis pathways | PloS one | 23755206 | nd by the vendors and subjected to in vitro assays between passages 8∼15, the cell lines for animal studies were between passages 5∼10. Human umbilical vein endothelial cell (HUVEC) obtained from Allcells (Emeryville, CA) was kept in EGM-2 (LONZA, Al | Full Paper |
2013 | Mendel, TA;Clabough, EB;Kao, DS;Demidova-Rice, TN;Durham, JT;Zotter, BC;Seaman, SA;Cronk, SM;Rakoczy, EP;Katz, AJ;Herman, IM;Peirce, SM;Yates, PA; | Pericytes derived from adipose-derived stem cells protect against retinal vasculopathy | PloS one | 23741506 | tient-derived lipoaspirate and further purified with serial passaging and sub-culturing, as we and others have previously shown [22]. Human bone marrow derived stem cells (hBMSCs) were purchased from Allcells (#MSC-001F). Bovine retinae and retinal m | Full Paper |
2013 | Su, RJ;Baylink, DJ;Neises, A;Kiroyan, JB;Meng, X;Payne, KJ;Tschudy-Seney, B;Duan, Y;Appleby, N;Kearns-Jonker, M;Gridley, DS;Wang, J;Lau, KH;Zhang, XB; | Efficient generation of integration-free ips cells from human adult peripheral blood using BCL-XL together with Yamanaka factors | PloS one | 23704989 | ied by MACS as described previously. [40] MNCs were obtained by standard density gradient centrifugation with Ficoll-Hypaque (1.077 g/ml) at room temperature. Some PBMNCs were directly purchased from AllCells (Emeryville, CA). The age of male and fem | Full Paper |
2013 | Evseenko, D;Latour, B;Richardson, W;Corselli, M;Sahaghian, A;Cardinal, S;Zhu, Y;Chan, R;Dunn, B;Crooks, GM; | Lysophosphatidic acid mediates myeloid differentiation within the human bone marrow microenvironment | PloS one | 23696850 | emical analyses. The murine stromal cell lines MS5 and OP9 (American Type Culture Collection (ATCC, Rockville, MD, USA) or primary human bone marrow derived mesenchymal stromal cells (passage 2-3) (AllCells Inc, Emeryville CA) were seeded into chambe | Full Paper |
2013 | Melton, AC;Melrose, J;Alajoki, L;Privat, S;Cho, H;Brown, N;Plavec, AM;Nguyen, D;Johnston, ED;Yang, J;Polokoff, MA;Plavec, I;Berg, EL;O'Mahony, A; | Regulation of IL-17A production is distinct from IL-17F in a primary human cell co-culture model of T cell-mediated B cell activation | PloS one | 23505568 | Frozen vials of positively selected primary normal human CD19+ B cells and negatively selected CD4+ T cells were obtained from AllCells (Emeryville, CA). PBMC were isolated from Save | Full Paper |
2013 | Angevine, K;Wuescher, L;Mensah-Osman, E; | Loss of menin mediated by endothelial cells treated with CoPP is associated with increased maturation of adipocytes | Adipocyte | 24052896 | , 10 mM l-glutamine, 0.75 units/mL heparin sulfate, 1 μg/mL hydrocortisone, 50 μg/mL ascorbic acid, and 2% fetal bovine serum. Bone marrow derived mesenchymal stem cells (MSCs) were purchased from AllCells and maintained at 37 °C and 5% CO2 in α-mem | Full Paper |
2013 | Omana-Zapata, I;Oreizy, F;Mosqueda, F;Salemenik, M;Lin, A;Wen, JL;Varro, R;Lee-Haynes, L;Segurado, OG; | Performance of a novel BD stem cell enumeration kit on two flow cytometry systems | International journal of laboratory hematology | 23190563 | Blood collection program and one frozen leukapheresis aliquot were enrolled for Linearity. Twenty-seven fresh leukapheresis aliquots from healthy mobilized adult donors were enrolled in the stability evaluation. All leukapheresis aliquots, fresh and | Full Paper |
2013 | Seki, K;Hisada, T;Kawata, T;Kamide, Y;Dobashi, K;Yamada, M;Mori, M;Okajima, F;Ishizuka, T; | Oxidative stress potentially enhances FcεRI-mediated leukotriene C4 release dependent on the late-phase increase of intracellular glutathione in mast cells | Biochemical and biophysical research communications | 23998930 | Human cord blood CD34 + cells were purchased from ALLCELLS (Emeryville, CA) and cultured in complete medium consisting of RPMI1640 (Life Technologies, Carlsbad, CA) Save | Full Paper |
2013 | Li, S;Garrett-Bakelman, FE;Akalin, A;Zumbo, P;Levine, R;To, BL;Lewis, ID;Brown, AL;D'Andrea, RJ;Melnick, A;Mason, CE; | An optimized algorithm for detecting and annotating regional differential methylation | BMC bioinformatics | 23735126 | Ten acute myeloid leukemia (AML) de-identified patient samples enriched for myeloblast cells and five normal bone marrow (NBM) samples (purchased from AllCells) were used in the Save | Full Paper |
2013 | Ohkawa, S;Kamei, N;Kamei, G;Shi, M;Adachi, N;Deie, M;Ochi, M; | Magnetic targeting of human peripheral blood CD133+ cells for skeletal muscle regeneration | Tissue engineering. Part C, Methods | 23298291 | )-mobilized human peripheral blood-derived-MNCs by using a direct immunomagnetic CD133 MicroBeads labeling and a magnetic cell sorting system, were purchased from AllCells( Save | Full Paper |
2013 | Kim, J;Zanjani, ED;Jeanblanc, CM;Goodrich, AD;Hematti, P; | Generation of CD34+ cells from human embryonic stem cells using a clinically applicable methodology and engraftment in the fetal sheep model | Experimental hematology | 23612043 | For generation of BM CD14 + cell-derived macrophages, CD14 + cells were isolated from BM aspirates of normal donors (AllCells) and harvested after 1 week (n = 6). For generation of Save | Full Paper |
2013 | Cherukuri, A;Patton, K;Gasser, RA;Zuo, F;Woo, J;Esser, MT;Tang, RS; | Adults 65 years old and older have reduced numbers of functional memory T cells to respiratory syncytial virus fusion protein | Clinical and vaccine immunology : CVI | 23239796 | A pooled human reference plasma standard (AllCells, LLC, Emeryville, CA) was included on each plate for comparison, and all plates were incubated for 1 h. A SulfoTag-labeled goat Save | Full Paper |
2013 | Kim, DH;Liu, J;Bhat, S;Benedict, G;Lecka-Czernik, B;Peterson, SJ;Ebraheim, NA;Heck, BE; | Peroxisome proliferator-activated receptor delta agonist attenuates nicotine suppression effect on human mesenchymal stem cell-derived osteogenesis and involves increased expression of heme oxygenase-1 | Journal of bone and mineral metabolism | 22945906 | Frozen bone marrow mononuclear cells were purchased from AllCells (Emeryville, CA, USA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential Save | Full Paper |
2013 | Li, L;Wang, Y;Chen, J;Cheng, B;Hu, J;Zhou, Y;Gao, X;Gao, L;Mei, X;Sun, M;Zhang, Z;Song, H; | An Engineered Arginase FC Protein Inhibits Tumor Growth In Vitro and In Vivo | Evidence-based complementary and alternative medicine : eCAM | 23737831 | umbilical cord vein were purchased from Allcells Inc. A confluent monolayer of synchronized HUVECS was scraped with a razor blade as described in [14]. After wounding, the cells Save | Full Paper |
2013 | Tummala, S;Titus, M;Wilson, L;Wang, C;Ciatto, C;Thill, G;Foster, D;Li, C;Szabo, Z;Guttman, A;Bettencourt, B;Jayaraman, M;Deroot, J;Kocisko, D;Pollard, S;Charisse, K;Kuchimanchi, S;Hinkle, G;Milstein, S;Meyers, R;Wu, SL;Karger, BL;Rossomando, A; | Evaluation of exogenous siRNA addition as a metabolic engineering tool for modifying biopharmaceuticals | Biotechnology progress | 23172735 | 12K media containing 10% fetal bovine serum. Fresh human normal PBMCs were purchased from AllCells (Emeryville, CA). Approximately 1 ×105 Jeko-1 cells were pre-incubated with RNAi-treated or control antibodies at varying concentrations ranging from 0 | Full Paper |
2013 | Rocha, LR;Veiga, DF;e Oliveira, PR;Song, EH;Ferreira, LM; | Health Service Quality Scale: Brazilian Portuguese translation, reliability and validity | BMC health services research | 23327598 | Business School, Avinguda de Pearson, 21, 08034, Barcelona, Spain Find articles by Paulo Rocha e Oliveira [https://pubmed.ncbi.nlm.nih.gov/?term=e%20Oliveira%20PR%5BAuthor%5D] ELAINE HORIBE SONG 4AllCells LLC, 5858 Horton Street, Emeryville, CA, 9 | Full Paper |
2013 | Ross, KM;McDonald-Jones, G;Miller, GE; | Oxytocin does not attenuate the ex vivo production of inflammatory cytokines by lipopolysaccharide-activated monocytes and macrophages from healthy male and female donors | Neuroimmunomodulation | 23899661 | For MP experiments, we purchased MO-derived, in vitro matured MPs from a cell supplier (AllCells, Emeryville, CA). All MPs used here were matured from a single donor's peripheral Save;Background: Oxytocin (OT) is a neuropeptide shown to attenuate i | Full Paper |
2013 | Wu, Y;Yang, Y;Yang, P;Gu, Y;Zhao, Z;Tan, L;Zhao, L;Tang, T;Li, Y; | The osteogenic differentiation of PDLSCs is mediated through MEK/ERK and p38 MAPK signalling under hypoxia | Archives of oral biology | 23806288 | The bone marrow stromal stem cells (BMSSCs) used as controls were obtained from a commercially available resource (AllCells LLC, Berkeley, CA, USA). Save | Full Paper |
2013 | Miller, P; | Stabilization of memory States by stochastic facilitating synapses | Journal of mathematical neuroscience | 24314108 | S is a scaled version of s, accounting for the totalfeedback conductance, S = W s , where W is the sum of connection strengths of allcells and held fixed when N is varied. Save | Full Paper |
2013 | Zhan, H;Cardozo, C;Yu, W;Wang, A;Moliterno, AR;Dang, CV;Spivak, JL; | MicroRNA deregulation in polycythemia vera and essential thrombocythemia patients | Blood cells, molecules & diseases | 23265742 | the peripheral blood CD34+ cells were 95% positive both for CD34 and CD38, were dimly positive for CD117, were CD41 negative, and were primarily in G0/G1 of the cell cycle [31]. Peripheral blood CD34+ cells from healthy controls were purchased from A | Full Paper |
2013 | Li, H;Xie, W;Gore, ER;Montoute, MN;Bee, WT;Zappacosta, F;Zeng, X;Wu, Z;Kallal, L;Ames, RS;Pope, AJ;Benowitz, A;Erickson-Miller, CL; | Development of phenotypic screening assays for γ-globin induction using primary human bone marrow day 7 erythroid progenitor cells | Journal of biomolecular screening | 24163393 | All cryopreserved human bone marrow CD34 + cells used here were obtained from AllCells (Emeryville, CA) and were generally from different donors. The CD34 + cells were cultured Save | Full Paper |
2013 | Schwimmer, LJ;Huang, B;Giang, H;Cotter, RL;Chemla-Vogel, DS;Dy, FV;Tam, EM;Zhang, F;Toy, P;Bohmann, DJ;Watson, SR;Beaber, JW;Reddy, N;Kuan, HF;Bedinger, DH;Rondon, IJ; | Discovery of diverse and functional antibodies from large human repertoire antibody libraries | Journal of immunological methods | 23454004 | The source RNA and cDNA used to amplify the V-genes was purchased from AllCells and Cureline. The E. coli strain TG1 (Lucigen) was used for all molecular cloning, phage Save | Full Paper |
2013 | Rosa, M;Pragasam, P;Saremian, J;Aoalin, A;Graf, W;Mohammadi, A; | The unsatisfactory ThinPrep® Pap Test™: analysis of technical aspects, most common causes, and recommendations for improvement | Diagnostic cytopathology | 22833404 | Fourth slide displays "halo effect" in which allcells are located at the periphery of the slide. This represents a technical preparation problem that can be resolved in the majority of cases Save | Full Paper |
2013 | Vainer, ED;Meir, K;Furman, M;Semenenko, I;Konikoff, F;Vainer, GW; | Characterization of novel CD55 isoforms expression in normal and neoplastic tissues | Tissue antigens | 23692281 | RNA was obtained from Ambion (Austin, TX), Asterand (Detroit, MI), AllCells (Emeryville, CA), Genomics Collaborative (Seracare; Cambridge, MA), Biochain (Newark, CA) and Ichilov medical center (Tel-aviv, Israel). CRC cell lines: colo205, DLD-1, HCT11 | Full Paper |
2013 | Muluk, NB;Arikan, OK;Atasoy, P;Kiliç, R;Yalçinozan, ET; | Inducible nitric oxide synthase (iNOS) in sinonasal polyp pathogenesis | B-ENT | 24273952 | the allcells group of inflammatory cells, there were PMNCs, MNCs, and fibroblasts. Higher iNOS expression in the allcells Thus, iNOS expression in the allcells group was mainly due to Save | Full Paper |
2013 | Wagner, LA;Wang, S;Wayner, EA;Christensen, C;Perkins, SJ;Ward, GW;Weiss, RB;Dunn, DM;Redd, MJ;Spangrude, GJ;Gleich, GJ; | Developing and mature human granulocytes express ELP 6 in the cytoplasm | Human antibodies | 24284306 | R&D Systems) or 2 U/ml epoietin-α [18]. Collection of de-identified UCMC was approved by the University of Utah’s Institutional Review Board. Bone marrow mononuclear cells (BMMC) were purchased from AllCells (Emeryville, CA) | Full Paper |
2013 | Köllmer, M;Buhrman, JS;Zhang, Y;Gemeinhart, RA; | Markers Are Shared Between Adipogenic and Osteogenic Differentiated Mesenchymal Stem Cells | Journal of developmental biology and tissue engineering | 24013643 | Human bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque TM PLUS solution followed by cell Save | Full Paper |
2013 | Hu, K;Slukvin, I; | Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors | Methods in molecular biology (Clifton, N.J.) | 23546755 | Human cord blood mononuclear cells (AllCells, CA, USA). 12. Human bone marrow mononuclear cells (AllCells, CA, USA). 13. Human bone marrow cells from patient with CML (AllCells, CA, USA). 14. Human cord blood CD34+ cells (AllCells, CA, USA). 15. | Full Paper |
2013 | Chu, EM;Tai, DC;Beer, JL;Hill, JS; | Macrophage heterogeneity and cholesterol homeostasis: classically-activated macrophages are associated with reduced cholesterol accumulation following treatment with oxidized LDL | Biochimica et biophysica acta | 23142249 | CD14 + human peripheral blood monocytes (AllCells) were cultured in RPMI 1640 complete media (Hyclone) containing 10% FBS (Hyclone), 2% sodium bicarbonate, 1% sodium Save | Full Paper |
2013 | Arora, M; | Cell Culture Media: A Review | Materials and Methods | ary cells. CELLS MEDIA Endothelial cells Human Endothelial-SFM (Life Technologies), Endothelial Basal Media, EndoGRO-LS Complete Media Kit (MilliporeSigma), HUVEC Basal Medium CB HUVEC (AllCells), Endothelial Cell Medium (ScienCell) | Full Paper | |
2013 | Mirshokraie, S;Mesbah, A;Pattabiraman, K; | Efficient JavaScript Mutation Testing | 2013 IEEE Sixth International Conference on Software Testing, Verification and Validation | function calls setup to set the dimension of all DOM elements with a class name of allCells; Assuming that the number of DOM elements with class name allCells is 9 (line 3 in Figure 1), Save | Full Paper | |
2013 | Fox, S;Adachi, T;Stillwell, W; | A QUINONE-ASSISTED PHOTOFORMATION OF ENERGY-RICH CHEMICAL BONDS | Solar Energy International Progress | In severalconferences , biochemists , chemicalengineers , andothershavediscusse dhow to industrializephotosyn thesis and how to industrializethe ATP cyclethatoperates in allcells . T Save | Full Paper | |
2013 | Margules, D;Flynn, J;Cooper, C; | CALCITONIN-LIKE PEPTIDE IN PRO-OPIOCORTIN: FUNCTIONAL ASPECTS | Advances in Endogenous and Exogenous Opioids | cells of the anterior pituitary and in allcells of the intermediate pituitary . EXCESS IN OBESE cells of the anterior pituitary and in allcells of the intermediate pituitary . EXCESS IN OBESE Save | Full Paper | |
2013 | Brodie, G; | CONSTRUCTED WETLANDS FOR TREATING ACID DRAINAGE AT TENNESSEE VALLEY AUTHORITY COAL FACILITIES | Constructed Wetlands in Water Pollution Control | S tagedtreatmentusingse veralcells may providea more efficientsystemsincec hemical and hydraulicoverloading of allcells would be minimized. Acid drainage treatment Water depth Save | Full Paper | |
2013 | Masuda, H;Asahara, T; | Isolation of Colony-Forming Endothelial Progenitor Cells | Manual of Research Techniques in Cardiovascular Medicine | approval of the institutional ethical committees of the Cord Blood and/or Clinical Investigation Committee. Alternatively, isolated MNCs, CD34+ , or CD133+ cells in UCB, PB, or BM can be purchased (e.g. ALLCELLSTM: http://www.allcells.com) | Full Paper | |
2013 | Drew, H; | Analysis of Macrophage- | Thesis | LPS, bovine CS and bovine aggrecan were purchased from SigmaAldrich (St. Louis, MO). Human monocytes (AllCells, LCC, Emeryville, CA) were cultured in RPMI-1640 with 10% fetal bovine serum. Bovine keratan sulfate proteoglycan (KSPG) was purchased f | Full Paper | |
2013 | Adams, GN; | Prolylcarboxypeptidase protects from vascular dysfunction and promotes vascular repair | Thesis | HUVEC were purchased from AllCells (Emeryville, CA) and grown on gelatin coated plates in CSC media (Cells Systems; Kirkland, WA). Cells were grown to near confluence in 6-well plates and transfected with Lipofectamine 2000 reagent (Invitrogen) in OP | Full Paper | |
2013 | Patel, VM; | Investigating apoptosis pathway in chronic lymphocytic leukemia: stromal influence and therapeutic activation | Thesis | All subjects signed written informed consent forms in accordance with the Declaration of Helsinki, and the laboratory protocols were approved by the institutional review board (IRB) at the University of Texas MD Anderson Cancer Center. Normal B‐lymph | Full Paper | |
2013 | Yi, S; | Green Manufacturing of Nanoparticles for Biomedical Applications | Thesis | Splenocytes, derived from C57BL/6 mice, were purchased from the Allcells Company (Emeryville, CA). HEPES, 1, 9-dimethyl-methylene blue (DMMB), chondroitin sulfate (CS), Sephadex G75, DEAE-cellulose and phosphate buffered saline (PBS) were purchased f | Full Paper | |
2013 | Adams, KJ; | Redirected T cell activity by high affinity TCR-ANTI-CD3 bispecific candidate therapeutics | Thesis | 2.2.2.3 Frozen peripheral blood mononuclear cells (PBMC) derived from multiple myeloma patients PBMC derived from multiple myeloma patients were purchased from AllCells as frozen aliquots, and were thawed and used on the day of the assay | Full Paper | |
2013 | PURWANTI, YIDA; | CANCER IMMUNOTHERAPY: TARGETED CELLULAR VEHICLE-MEDIATED IMMUNOGENE THERAPY AND DENDRITIC CELL-BASED VACCINE | Thesis | DCs and naïve T cells were derived from human peripheral blood mononuclear cells (PBMCs), both HLA-A2 positive and negative (AllCells, Stemcell Technologies Inc) based on previous studies 178. To generate DCs, 1×108 human PBMCs were seeded onto a T-1 | Full Paper | |
2013 | Kalim, KW; | The role of immunoproteasome subunit LMP7 in modulating T-helper cell differentiation and progression of autoimmune diseases | Thesis | cells were obtained from ATCC (Manassas, VA) and cultured in media recommended by the supplier. Human peripheral blood mononuclear cells (PBMC) from normal healthy volunteers were purchased from AllCells (Emeryville, CA) and those from individuals wi | Full Paper | |
2013 | Suknuntha, K; | An Induced Pluripotent Stem Cell-Based Platform for In Vitro Study of Chronic Myeloid Leukemia Stem Cell Development and Drug Resistance | Thesis | cells from CML patients in the chronic phase were purchased commercially (AllCells or in the chronic phase were purchased commercially (AllCells or Applied StemCells), or obtained Save | Full Paper | |
2013 | O'Huallachain, ME; | Genomic Variation in Somatic Human Tissues | Thesis | for a complexrearrangement in the PMP22 gene in an unaffected parent of a patient affect byCharcot-Marie-Tooth disease who had the same rearrangement throughout allcells. Save | Full Paper | |
2013 | Bhat, S; | Impact of nicotine and PPARδ-agonist on human mesenchymal stem cells | Thesis | An overview of all experiments performed in this thesis is provided on the previous page (see Figure 7). The overview includes type of experiment, treatments and assay/staining procedures conducted. Frozen (in 10%DMSO) bone marrow mononuclear cells ( | Full Paper | |
2013 | Kadam, S; | Global Landscape of DNA Methylation and Gene Expression in Hematopoiesis and Monocytic Differentiation | Thesis | Table 1: Information about the samples obtained from AllCells Inc; age, sex, ethnicity, lot number, catalog number, and location of cell (Bone Marrow and Peripheral Blood). RNA Expression Analysis in the Hematopoetic Lineage Even though DNA methylati | Full Paper | |
2013 | Kohn, LA; | Identification and Characterization of the Earliest Stages of Human Lymphoid Commitment | Thesis | Tissue Core of the University of California, Los Angeles, Cincinnati Children’s Hospital, or AllCells according to guidelines approved by Institutional Review Board of the University of California, Los Angeles. Samples were enriched for CD34+ cells | Full Paper | |
2013 | Haworth, KG; | Molecular Characterization of the HIV-1 Vpu Protein and Its Role in Antagonizing the Cellular Restriction Factor BST-2/Tetherin Both In Vitro and In Vivo | Thesis | Human peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA), depleted of CD8+ T cells using a positive selection CD8 microbead isolation kit (Miltenyi Biotech, Gladbach, Germany), and cultured in supplemented R10 medi | Full Paper | |
2013 | Cullimore, M; | Establishing a role for IL-27 in the amelioration of MS and EAE | Thesis | Whole venous blood was collected in EDTA-treated tubes from healthy donors after their informed consent. PBMCs were isolated using FiColl-Paque Plus™ density centrifugation while cord blood cells were obtained from AllCells. CD4+ /CD14+ cells were pu | Full Paper | |
2013 | Johnston, JM; | Optimization of a Lentiviral Vector for use in an HSC Gene Therapy Protocol for Hemophilia A | Thesis | CD34+ cells were purchased from AllCells (Emeryville, CA). Prior to arrival, CD34+ cells were mobilized from human bone marrow and isolated from the peripheral blood of 3 individual subjects. Flow cytometry was used to assess the purity of the sample | Full Paper | |
2013 | Xiao, L; | Dendritic cell-specific vaccine utilizing antibody-mimetic ligand and lentivector system | Thesis | Human PBMC-derived DCs were generated and purified as previously described,(Romani and others 1994; Sallusto and others 1994) except that human PBMCs were from HLA-A2+ patients (AllCells, LLC.). Briefly, plastic-adherent cell fractions of human PBMCs | Full Paper | |
2013 | Ji, T;Lin, C;Krill, LS;Eskander, R;Guo, Y;Zi, X;Hoang, BH; | Flavokawain B, a kava chalcone, inhibits growth of human osteosarcoma cells through G2/M cell cycle arrest and apoptosis | Molecular cancer | 23764122 | number of 2×104 cells were mixed with FKB or Adriamycin at concentration of 8.8 nM/ml, 17.6 nM/ml and 26.4 nM/ml, respectively. The mixture was cultured in 1 ml ColonyGel 1201 Mouse Base Medium (Reachbio) using a 6-well plate under standard culture | Full Paper |
2013 | Lee, YT;de Vasconcellos, JF;Yuan, J;Byrnes, C;Noh, SJ;Meier, ER;Kim, KS;Rabel, A;Kaushal, M;Muljo, SA;Miller, JL; | LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivo | Blood | 23798711 | All related studies were performed after human subjects review and National Institutes of Health Institutional Review Board approval. These studies were conducted in accordance with the Declaration of Helsinki. Human cord blood CD34+ cells were obtai | Full Paper |
2013 | Brandao, LN;Winges, A;Christoph, S;Sather, S;Migdall-Wilson, J;Schlegel, J;McGranahan, A;Gao, D;Liang, X;Deryckere, D;Graham, DK; | Inhibition of MerTK increases chemosensitivity and decreases oncogenic potential in T-cell acute lymphoblastic leukemia | Blood cancer journal | 23353780 | w cytometer and CXP data analysis software (Beckman Coulter). Methylcellulose assay Five hundred Jurkat parental and shRNA derivative cells were plated in methylcellulose-based medium (Cat no. 1101, Reachbio, Seattle, WA, USA) according to manufactu | Full Paper |
2013 | Grandi, FC;Rosser, JM;An, W; | LINE-1-derived poly(A) microsatellites undergo rapid shortening and create somatic and germline mosaicism in mice | Molecular biology and evolution | 23125228 | Cell and viability counts were performed using glacial acetic acid and trypan blue. Bone marrow cells were plated in ColonyGEL Complete Medium Without Epo (ReachBio LLC) at a density of 2 × 104 or 1 × 104 cells in 35 mm cell culture dishes and cultur | Full Paper |
2013 | Ulyanova, T;Jiang, Y;Padilla, SM;Papayannopoulou, T; | Erythroid cells generated in the absence of specific β1-integrin heterodimers accumulate reactive oxygen species at homeostasis and are unable to mount effective antioxidant defenses | Haematologica | 23812936 | CFU-C assays To quantify committed progenitors of all lineages, 50,000 BM cells, 500,000 splenic cells or 50-250 microliters of peripheral blood were cultured in semi-solid methylcellulose medium supplemented with cytokines (ReachBio) for seven days | Full Paper |
2013 | Zeiger, AS;Hinton, B;Van Vliet, KJ; | Why the dish makes a difference: quantitative comparison of polystyrene culture surfaces | Acta biomaterialia | 23454055 | Human bone-marrow-derived mesenchymal stromal or “stem” cells (hMSCs; ReachBio Seattle, WA) were expanded until passages 4-6, and plated onto P35 TCPS dishes at low density (∼5000 cells cm−2) to maintain subconfluent culture conditions | Full Paper |
2013 | Lee-Sherick, AB;Eisenman, KM;Sather, S;McGranahan, A;Armistead, PM;McGary, CS;Hunsucker, SA;Schlegel, J;Martinson, H;Cannon, C;Keating, AK;Earp, HS;Liang, X;DeRyckere, D;Graham, DK; | Aberrant Mer receptor tyrosine kinase expression contributes to leukemogenesis in acute myeloid leukemia | Oncogene | 23474756 | For methylcellulose colony forming assays, cells were plated in triplicate at a density of 500 cells/plate in Colony Gel Human Base Medium methylcellulose (Reach Bio, Seattle, WA, USA) with RPMI media containing 10% FBS added per manufacturer instruc | Full Paper |
2013 | Rossetto, CC;Tarrant-Elorza, M;Pari, GS; | Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cells | PLoS pathogens | 23717203 | d, 10 ng/ml TPO, and 10 ng/ml TNF for three days, followed by the addition of 50 ng/ml lipopolysaccharide (LPS) (Sigma-Aldrich) for an additional four days. Human CD14 (+) cells purchased (Lonza and ReachBio) or isolated from cord blood were maintai | Full Paper |
2013 | Christoph, S;Deryckere, D;Schlegel, J;Frazer, JK;Batchelor, LA;Trakhimets, AY;Sather, S;Hunter, DM;Cummings, CT;Liu, J;Yang, C;Kireev, D;Simpson, C;Norris-Drouin, J;Hull-Ryde, EA;Janzen, WP;Johnson, GL;Wang, X;Frye, SV;Earp, HS;Graham, DK; | UNC569, a novel small-molecule mer inhibitor with efficacy against acute lymphoblastic leukemia in vitro and in vivo | Molecular cancer therapeutics | 23997116 | Jurkat or 697 cells were plated in methylcellulose-based medium (#1101; ReachBio) containing UNC569 or DMSO control according to the manufacturer's protocol (500 cells/mL Save | Full Paper |
2013 | Bausch, E;Kohlhof, H;Hamm, S;Krauss, R;Baumgartner, R;Sironi, L; | A novel microtubule inhibitor 4SC-207 with anti-proliferative activity in taxane-resistant cells | PloS one | 24324550 | Mouse femoral bone marrow cells derived from an 8 week old Balb/c mouse were qualified at ReachBio (in terms of progenitor frequency), and stored at -152C. Cells were prepared as Save;the absorbance at time zero (drug addition), Ti the absorbance a | Full Paper |
2013 | Weerasinghe, P;Hallock, S;Brown, RE;Loose, DS;Buja, LM; | A model for cardiomyocyte cell death: insights into mechanisms of oncosis | Experimental and molecular pathology | 22609242 | )-derived cardiomyocytes were purchased from Reach Bio LLC (Seattle, WA) and neonatal Sanguinarine was subsequently diluted with cardiomyocyte growth media (Reach Bio LLC, Save | Full Paper |
2013 | Maloney, JM;Lehnhardt, E;Long, AF;Van Vliet, KJ; | Mechanical fluidity of fully suspended biological cells | Biophysical journal | 24138852 | Primary adult human mesenchymal stem cells (hMSCs) were isolated from the bone marrow of four adult donors via Stem Cell Technologies (Vancouver, BC, Canada), ReachBio (Seattle, WA), or Lonza Group (Basel, Switzerland) | Full Paper |
2013 | Whitfield, MJ;Lee, WC;Van Vliet, KJ; | Onset of heterogeneity in culture-expanded bone marrow stromal cells | Stem cell research | 24103495 | BMSCs used for long-term imaging were purchased from ReachBio, and received at passage 1 (p1). Cells were seeded at ~ 2000 cells/cm 2 on tissue culture-treated polystyrene (BD Save | Full Paper |
2013 | Pierce, T; | Keeping Piracy to a Minimum | Appreneur | Two assay platforms, specifically designed for ADCs, have been developed by ReachBio as a result of increasing demand by industry for ways to assess the potential toxicity of these Save | Full Paper | |
2013 | Kennedy, JP; | A cellularized biomaterial model of cardiac fibroblasts for evaluation of fibroblast induced changes in stiffness | Thesis | Single donor human mesenchymal stem cells (MSC; ReachBio Seattle, WA) were passaged 2-3, and expression of CD29, CD73, CD105 CD34, and CD45 surface markers was evaluated by FACS (flow cytometry performed by manufacturer). U | Full Paper | |
2013 | Rosser, JM; | Investigating DNA methylation dynamics with transgenic LINE-1 mouse models | Thesis | Cell and viability counts were performed using glacial acetic acid and trypan blue. Bone marrow cells were plated in ColonyGEL Complete Medium Without Epo (ReachBio LLC) at a density | Full Paper | |
2012 | Xu, B;Ionita-Laza, I;Roos, JL;Boone, B;Woodrick, S;Sun, Y;Levy, S;Gogos, JA;Karayiorgou, M; | De novo gene mutations highlight patterns of genetic and neural complexity in schizophrenia | Nat. Genet. | 23042115 | We are enormously grateful to all the families who participated in this research. We thank H. Pretorius and nursing sisters R. van Wyk, C. Botha and H. van den Berg for their assistance with subject recruitment, family history assessments and diagnos | Full Paper |
2012 | Yang, J;Ramos, E;Corces, VG; | The BEAF-32 insulator coordinates genome organization and function during the evolution of Drosophila species | Genome Res. | 22895281 | on a Branson Sonifier 250 with output control set at 1.5. Libraries were prepared with the Illumina TruSeq DNA Sample Preparation Kit and sequenced at the HudsonAlpha Institute for Biotechnology. | Full Paper |
2012 | Van Bortle, K;Ramos, E;Takenaka, N;Yang, J;Wahi, JE;Corces, VG; | Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domains | Genome Res. | 22722341 | Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (NEB Cat# F-530L) and size selected for 200–300 bp by gel extraction. ChIP libraries were sequenced at the HudsonAlpha Institute for Biotechnology, using an Ill | Full Paper |
2012 | Kellner, WA;Ramos, E;Van Bortle, K;Takenaka, N;Corces, VG; | Genome-wide phosphoacetylation of histone H3 at Drosophila enhancers and promoters | Genome Res. | 22508764 | We thank Mattias Mannervik for the gift of CBP antibody and members of the Corces Lab for invaluable discussions. We also thank The Genomic Services Lab at the HudsonAlpha Institute for Biotechnology for their help in performing Illumina sequencing o | Full Paper |
2012 | Higgins, G;Roper, KM;Watson, IJ;Blackhall, FH;Rom, WN;Pass, HI;Ainscough, JF;Coverley, D; | Variant Ciz1 is a circulating biomarker for early-stage lung cancer | Proc. Natl. Acad. Sci. U.S.A. | 23074256 | ... anti-rabbit Alexa Fluor 488 (Invitrogen, Folio Bioscience). Subcellular fractionation was carried out as described (21). For immunohistochemistry, 2B was applied to SCLC tissue microarray ARY-HH0117 (Folio Biosciences) and visualized using Ultrav | Full Paper |
2012 | Fabbri, M;Paone, A;Calore, F;Galli, R;Gaudio, E;Santhanam, R;Lovat, F;Fadda, P;Mao, C;Nuovo, GJ;Zanesi, N;Crawford, M;Ozer, GH;Wernicke, D;Alder, H;Caligiuri, MA;Nana-Sinkam, P;Perrotti, D;Croce, CM; | MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory response | Proc. Natl. Acad. Sci. U.S.A. | 22753494 | We thank Phylogeny, Inc. for performing in situ hybridization in its entirety and locked nucleic acid in situ hybridization experiments and for supporting these experiment; Dr. Cecilia FernandezCymering and Dr. Stefano Volinia for supervision of stat | Full Paper |
2012 | Ren, H;Tan, ZP;Zhu, X;Crosby, K;Haack, H;Ren, JM;Beausoleil, S;Moritz, A;Innocenti, G;Rush, J;Zhang, Y;Zhou, XM;Gu, TL;Yang, YF;Comb, MJ; | Identification of anaplastic lymphoma kinase as a potential therapeutic target in ovarian cancer | Cancer Res. | 22570254 | Histologic typing and grading were carried out according to International Federation of Gynecology and Obstetrics (FIGO) guidelines. General pathologic information of the 69 ovarian tumor patients is listed in Supplementary Table S1. FFPE ovarian tum | Full Paper |
2012 | Polisetty, RV;Gautam, P;Sharma, R;Harsha, HC;Nair, SC;Gupta, MK;Uppin, MS;Challa, S;Puligopu, AK;Ankathi, P;Purohit, AK;Chandak, GR;Pandey, A;Sirdeshmukh, R; | LC-MS/MS analysis of differentially expressed glioblastoma membrane proteome reveals altered calcium signaling and other protein groups of regulatory functions | Mol. Cell Proteomics | 22219345 | ... tion [http://www.mcponline.org/cgi/content/full/M111.013565/DC1]. IMMUNOHISTOCHEMISTRY Formalin-fixed paraffin-embedded individual GBM tissue sections or commercially available tissue microarrays (Folio Biosciences, Columbus, OH) with 35 GBM t | Full Paper |
2012 | Gonzalez de Castro, D;Angulo, B;Gomez, B;Mair, D;Martinez, R;Suarez-Gauthier, A;Shieh, F;Velez, M;Brophy, VH;Lawrence, HJ;Lopez-Rios, F; | A comparison of three methods for detecting KRAS mutations in formalin-fixed colorectal cancer specimens | Br. J. Cancer | 22713664 | Materials: FFPET specimens from CRC tumours were purchased from US commercial vendors: Discovery Life Sciences, Inc. (Los Osos, CA, USA); BioServe (Beltsville, MD, USA); ProteoGenex (Culver City, CA, USA); CureLine, Inc | Full Paper |
2012 | Lenehan, PF;Boardman, LA;Riegert-Johnson, D;De Petris, G;Fry, DW;Ohrnberger, J;Heyman, ER;Gerard, B;Almal, AA;Worzel, WP; | Generation and external validation of a tumor-derived 5-gene prognostic signature for recurrence of lymph node-negative, invasive colorectal carcinoma | Cancer | 22605513 | (South San Francisco, Calif), Mayo Clinic (Rochester, Minn; Jacksonville, Fla; and Scottsdale, Ariz), Folio Biosciences (Columbus, Ohio), Trans‐Hit Biomarkers, Inc. (Montreal, Quebec, Canada), BioServe (Laurel, Md), and Amp‐Tec (Hamburg, Germany) | Full Paper |
2012 | Arrington, CB;Bleyl, SB;Matsunami, N;Bonnell, GD;Otterud, BE;Nielsen, DC;Stevens, J;Levy, S;Leppert, MF;Bowles, NE; | Exome analysis of a family with pleiotropic congenital heart disease | Circ Cardiovasc Genet | 22337856 | Exome sequencing (library construction, capture and sequencing) was carried out on 2 DNA samples (from patients III.3 and III.7) at the genomic service laboratory at HudsonAlpha Institute for Biotechnology (Huntsville, Alabama). Briefly, 3 to 5 µg of | Full Paper |
2012 | Dahmen, S;Haenni, M;Madec, JY; | IncI1/ST3 plasmids contribute to the dissemination of the blaCTX-M-1 gene in Escherichia coli from several animal species in France | J. Antimicrob. Chemother. | 22872449 | Characteristics of the eight CTX-M-1-producing E. coli isolates described in this study Strain Year District Animal Pathology b-Lactamases Additional resistancesa Phylogeny CTX-M-1 plasmid Inc group sequence type size (kb) 16170 2006 76 dog diarrhoea | Full Paper |
2012 | Fedyk, ER;Wyant, T;Yang, LL;Csizmadia, V;Burke, K;Yang, H;Kadambi, VJ; | Exclusive antagonism of the α4 β7 integrin by vedolizumab confirms the gut-selectivity of this pathway in primates | Inflamm. Bowel Dis. | 22419649 | Immunohistochemistry detecting the β7 integrin was conducted using a sheep antihuman β7 polyclonal antibody (R&D Systems, Minneapolis, MN) that does not compete with vedolizumab for binding to the α4β7 integrin expressed by leukocytes (data not shown | Full Paper |
2012 | Jenny, MJ;Aluru, N;Hahn, ME; | Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryos | Toxicol. Appl. Pharmacol. | 22921993 | The same total RNA used for the array hybridizations was also used for high-throughput sequencing using the SOLiD technology. Prior to sequencing, the quality of the RNA and presence of the microRNA fraction was confirmed with the Agilent Small RNA k | Full Paper |
2012 | Liu, S;Zhang, Y;Zhou, Z;Waldbieser, G;Sun, F;Lu, J;Zhang, J;Jiang, Y;Zhang, H;Wang, X;Rajendran, KV;Khoo, L;Kucuktas, H;Peatman, E;Liu, Z; | Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote | BMC Genomics | 23127152 | ;... urer's protocol as in previous study [5]. Equal amount of total RNA from each tissue was combined and sent out for commercial sequencing. Illumina sequencing Sequencing was conducted commercially in HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2012 | Rokyta, DR;Lemmon, AR;Margres, MJ;Aronow, K; | The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus) | BMC Genomics | 23025625 | Sequencing and nonnormalized cDNA library preparation were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially as described by Mort | Full Paper |
2012 | Sun, F;Peatman, E;Li, C;Liu, S;Jiang, Y;Zhou, Z;Liu, Z; | Transcriptomic signatures of attachment, NF-κB suppression and IFN stimulation in the catfish gill following columnaris bacterial infection | Dev. Comp. Immunol. | ; for use in RNA-seq. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) as previously described by Li et al. (2012). cDNA libraries were prepared with | Full Paper | |
2012 | Li, C;Zhang, Y;Wang, R;Lu, J;Nandi, S;Mohanty, S;Terhune, J;Liu, Z;Peatman, E; | RNA-seq analysis of mucosal immune responses reveals signatures of intestinal barrier disruption and pathogen entry following Edwardsiella ictaluri infection in channel catfish, Ictalurus punctatus | Fish Shellfish Immunol. | 22366064 | ; replicate control pool was formed for use in RNA-seq. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25 ug of | Full Paper |
2012 | Nguyen, TH;Weber, W;Havari, E;Connors, T;Bagley, RG;McLaren, R;Nambiar, PR;Madden, SL;Teicher, BA;Roberts, B;Kaplan, J;Shankara, S; | Expression of TMPRSS4 in non-small cell lung cancer and its modulation by hypoxia | Int. J. Oncol. | 22692880 | Immunohistochemistry. A tissue array containing 101 formalin-fixed, paraffin-embedded tissue cores of lung SCC, adenocarcinomas, and normal non-neoplastic lung tissues from multiple donors was used (Phylogeny Inc., Columbus, OH) | Full Paper |
2012 | Gurudatta, BV;Ramos, E;Corces, VG; | The BEAF insulator regulates genes involved in cell polarity and neoplastic growth | Dev. Biol. | 22743648 | ChIP libraries were sequenced at the HudsonAlpha Institute for Biotechnology, using an Illumina HiSeq system. Sequences were mapped to the dm3 genome with Bowtie 0.12.3 (Langmead et al., 2009) using default settings. Peaks were then called with MACS | Full Paper |
2012 | Lee, S;Brophy, VH;Cao, J;Velez, M;Hoeppner, C;Soviero, S;Lawrence, HJ; | Analytical performance of a PCR assay for the detection of KRAS mutations (codons 12/13 and 61) in formalin-fixed paraffin-embedded tissue samples of colorectal carcinoma | Virchows Arch. | 22173329 | Material and methods. Materials. FFPET specimens from CRC tumors were purchased from US commercial vendors: Discovery Life Sciences, Inc. (Los Osos, CA, USA); BioServe (Beltsville, MD, USA); ProteoGenex (Culver City, CA, USA); CureLine, Inc | Full Paper |
2012 | Hughes, JA;Lynch, FC; | Transhepatic removal of an inferior vena cava filter | J Vasc Interv Radiol | 22720899 | Full Paper | |
2012 | Lynch, FC; | Modified loop snare technique for the removal of bard recovery, G2, G2 express, and eclipse inferior vena cava filters | J Vasc Interv Radiol | 22525025 | Full Paper | |
2012 | Vijay, K;Hughes, JA;Burdette, AS;Scorza, LB;Singh, H;Waybill, PN;Lynch, FC; | Fractured Bard Recovery, G2, and G2 express inferior vena cava filters: incidence, clinical consequences, and outcomes of removal attempts | J Vasc Interv Radiol | 22173108 | Full Paper | |
2012 | Mutka, SC;Green, LH;Verderber, EL;Richards, JP;Looker, DL;Chlipala, EA;Rosenthal, GJ; | ADH IB expression, but not ADH III, is decreased in human lung cancer | PLoS ONE | 23285246 | GSNOR immunohistochemistry. Immunohistochemical analysis for GSNOR was performed using formalin-fixed, paraffin-embedded human lung tissue microarrays (Folio Bioscience, Columbus, OH; #ARY-HH0178, #ARY-HH0176) | Full Paper |
2012 | Ueki, A;Shimizu, T;Masuda, K;Yamaguchi, SI;Ishikawa, T;Sugihara, E;Onishi, N;Kuninaka, S;Miyoshi, K;Muto, A;Toyama, Y;Banno, K;Aoki, D;Saya, H; | Up-regulation of Imp3 confers in vivo tumorigenicity on murine osteosarcoma cells | PLoS ONE | 23226335 | Zeiss). Human Osteosarcoma Tissue Array An array of human osteosarcoma specimens was obtained from Folio Biosciences (Powell, OH) and was subjected to immunohis- tochemical staining for IMP3 as described above ; Human Osteosarcoma Tissue Array. | Full Paper |
2012 | Xu, J;Ji, P;Zhao, Z;Zhang, Y;Feng, J;Wang, J;Li, J;Zhang, X;Zhao, L;Liu, G;Xu, P;Sun, X; | Genome-wide SNP discovery from transcriptome of four common carp strains | PLoS ONE | 23110192 | ;... e distribution of all samples were checked with Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, USA). cDNA Library Construction Illumina Sequencing Sequencing of 24 samples was conducted in HudsonAlpha Genomic Services Laboratory (Hunt | Full Paper |
2012 | Hedin, M;Starrett, J;Akhter, S;Schönhofer, AL;Shultz, JW; | Phylogenomic resolution of paleozoic divergences in harvestmen (Arachnida, Opiliones) via analysis of next-generation transcriptome data | PLoS ONE | 22936998 | Protolophus total RNA was shipped to Cofactor Genomics (www.cofactorgenomics.com), where a duplex-specific nuclease (DSN) normalized library was constructed, and 60 basepair (bp) paired-end reads were generated using Illumina GAII technology. All oth | Full Paper |
2012 | Johansson, S;Irgens, H;Chudasama, KK;Molnes, J;Aerts, J;Roque, FS;Jonassen, I;Levy, S;Lima, K;Knappskog, PM;Bell, GI;Molven, A;Njølstad, PR; | Exome sequencing and genetic testing for MODY | PLoS ONE | 22662265 | Targeted capture and massive parallel sequencing were performed at HudsonAlpha Institute for Biotechnology (Huntsville, AL) (File S1). In brief, SureSelect Human All Exon Kit (Agilent Technologies, Santa Clara, CA) was used for exome enrichment, and | Full Paper |
2012 | Myers, RB;Rwayitare, K;Richey, L;Lem, J;Castellot, JJ; | CCN5 Expression in mammals. III. Early embryonic mouse development | J Cell Commun Signal | 22926930 | earlier, at the 4-cell stage. Materials and methods. Immunohistochemistry. 5 μm frozen sagittal sections of C57BL/6 post-implantation embryos were obtained from Folio Bio (Columbus, OH). Sections were cleared with xylene | Full Paper |
2012 | Ding, D;Scott, NM;Thompson, EE;Chaiworapongsa, T;Torres, R;Billstrand, C;Murray, K;Dexheimer, PJ;Ismail, M;Kay, H;Levy, S;Romero, R;Lindheimer, MD;Nicolae, DL;Ober, C; | Increased protein-coding mutations in the mitochondrial genome of African American women with preeclampsia | Reprod Sci | 22902742 | Mitochondrial genome libraries were prepared by amplification of the complete mtDNA genome using 2 sets of overlapping primers. These fragments were pooled in molar equivalent amounts, sonicated to randomly fragment the DNA, and then prepared for seq | Full Paper |
2012 | Morimoto, M;Souich, Cd;Trinh, J;McLarren, KW;Boerkoel, CF;Hendson, G; | Expression profile of NSDHL in human peripheral tissues | J. Mol. Histol. | 22113624 | This construct was cut at unique NotI and SpeI sites flanking the Nsdhl insert and transcribed by T3 and T7 RNA polymerases to yield antisense and sense in situ hybridization probes, respectively. In situ hybridization was performed by Phylogeny, Inc | Full Paper |
2012 | Wang, R;Li, C;Stoeckel, J;Moyer, G;Liu, Z;Peatman, E; | Rapid development of molecular resources for a freshwater mussel, Villosa lienosa (Bivalvia:Unionidae), using an RNA-seq-based approach | Freshwater Science | After quantitation with KAPA Library Quant Kits (Kapa Biosystems, Woburn, Massachusetts) and dilution, the libraries were clustered 4 per lane and sequenced on a HiSeq 2000 instrument with 100-bp PE reads at the HudsonAlpha Genomic Services Laborator | Full Paper | |
2012 | Kishida, M;Ishige, K;Horibe, T;Tada, N;Koibuchi, N;Shoda, J;Kita, K;Kawakami, K; | Orexin 2 receptor as a potential target for immunotoxin and antibody-drug conjugate cancer therapy | Oncol Lett | 22740944 | Materials and methods. Materials. Hepatocellular carcinoma tissue array, ARY- HH0075 was purchased from Folio Biosciences (Columbus, OH, USA). Digestive system disease tissue array, DID381 was purchased from US Biomax (Rockville, MD, USA) | Full Paper |
2012 | Kentsis, A;Reed, C;Rice, KL;Sanda, T;Rodig, SJ;Tholouli, E;Christie, A;Valk, PJ;Delwel, R;Ngo, V;Kutok, JL;Dahlberg, SE;Moreau, LA;Byers, RJ;Christensen, JG;Vande Woude, G;Licht, JD;Kung, AL;Staudt, LM;Look, AT; | Autocrine activation of the MET receptor tyrosine kinase in acute myeloid leukemia | Nature medicine | 22683780 | This report identifies upregulation of HGF as an autocrine growth pathway in several subsets of AML. Ligand-dependent activation of MET represents a new oncogenic stimulus, and the Save | Full Paper |
2012 | Schenk, T;Chen, WC;Göllner, S;Howell, L;Jin, L;Hebestreit, K;Klein, HU;Popescu, AC;Burnett, A;Mills, K;Casero, RA;Marton, L;Woster, P;Minden, MD;Dugas, M;Wang, JC;Dick, JE;Müller-Tidow, C;Petrie, K;Zelent, A; | Inhibition of the LSD1 (KDM1A) demethylase reactivates the all-trans-retinoic acid differentiation pathway in acute myeloid leukemia | Nature medicine | 22406747 | We grew normal bone marrow mononuclear cells (AllCells, ABM011F) in IMDM supplemented with 20 ng ml −1 SCF, 10 ng ml −1 each of IL-3, IL-6 and fms-related tyrosine kinase 3 ( Save | Full Paper |
2012 | Zhang, C;Srinivasan, Y;Arlow, DH;Fung, JJ;Palmer, D;Zheng, Y;Green, HF;Pandey, A;Dror, RO;Shaw, DE;Weis, WI;Coughlin, SR;Kobilka, BK; | High-resolution crystal structure of human protease-activated receptor 1 | Nature | 23222541 | In brief, acid-citrate-dextrose anti-coagulated human blood samples (60 ml per donor) were obtained from AllCells. Blood was centrifuged without braking at 250g at 37 C for 15 min. Save | Full Paper |
2012 | Sasaki, M;Knobbe, CB;Munger, JC;Lind, EF;Brenner, D;Brüstle, A;Harris, IS;Holmes, R;Wakeham, A;Haight, J;You-Ten, A;Li, WY;Schalm, S;Su, SM;Virtanen, C;Reifenberger, G;Ohashi, PS;Barber, DL;Figueroa, ME;Melnick, A;Zúñiga-Pflücker, JC;Mak, TW; | IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigenetics | Nature | 22763442 | Two normal CD34 + bone marrow control samples were purchased from AllCells. Institutional review board approval was obtained at Weill Cornell Medical Center and this study was Save | Full Paper |
2012 | Kohn, LA;Hao, QL;Sasidharan, R;Parekh, C;Ge, S;Zhu, Y;Mikkola, HK;Crooks, GM; | Lymphoid priming in human bone marrow begins before expression of CD10 with upregulation of L-selectin | Nature immunology | 22941246 | esis in disease states. METHODS Bone marrow cell isolation Normal human BM and thymic cells were obtained from healthy donors via the UCLA Pathology Tissue Core, Cincinnati Children’s Hospital, or ALLCELLS according to guidelines approved by UCLA In | Full Paper |
2012 | Zhu, X;Wang, Y;Pi, W;Liu, H;Wickrema, A;Tuan, D; | NF-Y recruits both transcription activator and repressor to modulate tissue- and developmental stage-specific expression of human γ-globin gene | PloS one | 23071749 | The human cells were obtained commercially from AllCells and processed with protocols approved by Human Assurance Committee of Georgia Health Sciences University (HAC file # Save | Full Paper |
2012 | Hur, EM;Patel, SN;Shimizu, S;Rao, DS;Gnanapragasam, PN;An, DS;Yang, L;Baltimore, D; | Inhibitory effect of HIV-specific neutralizing IgA on mucosal transmission of HIV in humanized mice | Blood | 23065154 | intraperitoneally with 3 × 10 6 human PBMCs (AllCells) that were activated by PHA (5 μg/mL) NSG mice were transplanted intraperitoneally with 3 × 10 6 human PBMCs (AllCells) that Save | Full Paper |
2012 | Lu, M;Wang, X;Li, Y;Tripodi, J;Mosoyan, G;Mascarenhas, J;Kremyanskaya, M;Najfeld, V;Hoffman, R; | Combination treatment in vitro with Nutlin, a small-molecule antagonist of MDM2, and pegylated interferon-α 2a specifically targets JAK2V617F-positive polycythemia vera cells | Blood | 22872685 | Fresh normal human bone marrow CD34+ cells were purchased from ALLCELLS. The characteristics of the PV patients studied are shown in supplemental Table 1 (available on the Blood Web site; see the Supplemental Materials link at the top of the online a | Full Paper |
2012 | Ndhlovu, LC;Lopez-Vergès, S;Barbour, JD;Jones, RB;Jha, AR;Long, BR;Schoeffler, EC;Fujita, T;Nixon, DF;Lanier, LL; | Tim-3 marks human natural killer cell maturation and suppresses cell-mediated cytotoxicity | Blood | 22383801 | Peripheral blood mononuclear cells (PBMCs) of healthy individuals were obtained from the Stanford Blood Bank. Cord blood PMBCs were obtained from AllCells. The University of California, San Francisco and the University of Hawaii institutional review | Full Paper |
2012 | Wey, S;Luo, B;Tseng, CC;Ni, M;Zhou, H;Fu, Y;Bhojwani, D;Carroll, WL;Lee, AS; | Inducible knockout of GRP78/BiP in the hematopoietic system suppresses Pten-null leukemogenesis and AKT oncogenic signaling | Blood | 21937694 | The normal peripheral blood mononuclear cells were purchased from ALLCELLS (catalog #PB003F). The ethical use of the human tissues for research was approved by the University Save | Full Paper |
2012 | Cheng, L;Hansen, NF;Zhao, L;Du, Y;Zou, C;Donovan, FX;Chou, BK;Zhou, G;Li, S;Dowey, SN;Ye, Z;NISC Comparative Sequencing Program, ;Chandrasekharappa, SC;Yang, H;Mullikin, JC;Liu, PP; | Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by nonintegrating plasmid expression | Cell stem cell | 22385660 | blood of anonymous donors were collected and processed at AllCells, LLC (Emeryville, CA). The by Institutional Review Board (IRB) at AllCells. Use of anonymous human samples for Save | Full Paper |
2012 | Smith, MR;Atkinson, P;White, D;Piersma, T;Gutierrez, G;Rossini, G;Desai, S;Wellinghoff, S;Yu, H;Cheng, X; | Design and assessment of a wrapped cylindrical Ca-P AZ31 Mg alloy for critical-size ulna defect repair | Journal of biomedical materials research. Part B, Applied biomaterials | 22102519 | AZ31 Mg alloy cupons were prepared as described above and sterilized using ethylene oxide gas. Two cell migration tests were performed: indirect and direct. Frozen human MSCs (AllCells, LLC, Emeryville, CA) were thawed, spun down, and resuspended in | Full Paper |
2012 | Muthuswamy, R;Berk, E;Junecko, BF;Zeh, HJ;Zureikat, AH;Normolle, D;Luong, TM;Reinhart, TA;Bartlett, DL;Kalinski, P; | NF-κB hyperactivation in tumor tissues allows tumor-selective reprogramming of the chemokine microenvironment to enhance the recruitment of cytolytic T effector cells | Cancer research | 22593190 | vein endothelial cells (HUVEC; AllCells) were cultured in HUVEC complete media (Basal media supplemented with HUVEC stimulatory supplement; AllCells). All cell lines were washed Save | Full Paper |
2012 | Capozza, F;Trimmer, C;Castello-Cros, R;Katiyar, S;Whitaker-Menezes, D;Follenzi, A;Crosariol, M;Llaverias, G;Sotgia, F;Pestell, RG;Lisanti, MP; | Genetic ablation of Cav1 differentially affects melanoma tumor growth and metastasis in mice: role of Cav1 in Shh heterotypic signaling and transendothelial migration | Cancer research | 22396494 | B16F10 and A-375 were from ATCC, while human immortalized dermal foreskin fibroblasts (hTBJ1) were originally from Clontech. Human Umbilical Vein Endothelial Cells (HUVEC) were purchased from ALLCELLS. Only early passages of these cell lines were use | Full Paper |
2012 | Silverman, AM;Nakata, R;Shimada, H;Sposto, R;DeClerck, YA; | A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma | Cancer research | 22389450 | Interleukin-6 (IL-6) is a pleiotropic cytokine with a broad range of physiologic and pathologic functions. Because in cancer, IL-6 contributes to a microenvironment that promotes tumor Save | Full Paper |
2012 | Pacienza, N;Yoshimitsu, M;Mizue, N;Au, BC;Wang, JC;Fan, X;Takenaka, T;Medin, JA; | Lentivector transduction improves outcomes over transplantation of human HSCs alone in NOD/SCID/Fabry mice | Molecular therapy : the journal of the American Society of Gene Therapy | 22472949 | Human mobilized PB CD34+ cells were obtained from AllCells (Emeryville, CA). These cells were rapidly thawed following the manufacturer's recommendations. After thawing, ~85% of the cells were viable as assessed by trypan blue exclusion, while the hC | Full Paper |
2012 | Lee, EJ;Choi, EK;Kang, SK;Kim, GH;Park, JY;Kang, HJ;Lee, SW;Kim, KH;Kwon, JS;Lee, KH;Ahn, Y;Lee, HJ;Cho, HJ;Choi, SJ;Oh, WI;Park, YB;Kim, HS; | N-cadherin determines individual variations in the therapeutic efficacy of human umbilical cord blood-derived mesenchymal stem cells in a rat model of myocardial infarction | Molecular therapy : the journal of the American Society of Gene Therapy | 22068423 | PBMCs (1 × 105/well) or hUCB-MSCs (1 × 102, 1 × 103 or 1 × 104/well) in 96-well tissue culture plates. The PBMCs were purchased from AllCells (Emeryville, CA) The stimulator PBMCs and hUCB-MSCs were inactivated by treatment with 10 µg/ml mitomycin-C | Full Paper |
2012 | Goh, KC;Novotny-Diermayr, V;Hart, S;Ong, LC;Loh, YK;Cheong, A;Tan, YC;Hu, C;Jayaraman, R;William, AD;Sun, ET;Dymock, BW;Ong, KH;Ethirajulu, K;Burrows, F;Wood, JM; | TG02, a novel oral multi-kinase inhibitor of CDKs, JAK2 and FLT3 with potent anti-leukemic properties | Leukemia | 21860433 | Primary cells—either peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients—were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex ( Save | Full Paper |
2012 | Boitano, AE;de Lichtervelde, L;Snead, JL;Cooke, MP;Schultz, PG; | An image-based screen identifies a small molecule regulator of megakaryopoiesis | Proceedings of the National Academy of Sciences of the United States of America | 22891346 | Human CD34+ cells were purified from fresh human leukophoresed-mobilized peripheral blood (AllCells) or from umbilical-cord blood (Bioreclamation), as noted, using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following manufacturer's | Full Paper |
2012 | Kobune, M;Iyama, S;Kikuchi, S;Horiguchi, H;Sato, T;Murase, K;Kawano, Y;Takada, K;Ono, K;Kamihara, Y;Hayashi, T;Miyanishi, K;Sato, Y;Takimoto, R;Kato, J; | Stromal cells expressing hedgehog-interacting protein regulate the proliferation of myeloid neoplasms | Blood cancer journal | 22961059 | Minneapolis, MN, USA). 5-Aza-2′-deoxycytidine (5-aza-dC) was obtained from Sigma (St Louis, MO, USA). Total RNA derived from mobilized peripheral blood (PB), BM or CB CD34+ cells was purchased from AllCells, LLC (Toronto, Ontario, Canada). Human myel | Full Paper |
2012 | Novotny-Diermayr, V;Hart, S;Goh, KC;Cheong, A;Ong, LC;Hentze, H;Pasha, MK;Jayaraman, R;Ethirajulu, K;Wood, JM; | The oral HDAC inhibitor pracinostat (SB939) is efficacious and synergistic with the JAK2 inhibitor pacritinib (SB1518) in preclinical models of AML | Blood cancer journal | 22829971 | Peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex (Culver City, CA, USA) Save | Full Paper |
2012 | Gao, N;Cheng, S;Budhraja, A;Gao, Z;Chen, J;Liu, EH;Huang, C;Chen, D;Yang, Z;Liu, Q;Li, P;Shi, X;Zhang, Z; | Ursolic acid induces apoptosis in human leukaemia cells and exhibits anti-leukaemic activity in nude mice through the PKB pathway | British journal of pharmacology | 21950524 | Blasts were suspended at 8 × 105 mL-1 and incubated in RPMI 1640 medium containing 10% FBS in 24-well plates. Fresh normal bone marrow mononuclear cells were purchased from Allcells (Emeryvill, CA). After being washed and counted, cells were suspende | Full Paper |
2012 | De Giovanni, C;Nicoletti, G;Landuzzi, L;Romani, F;Croci, S;Palladini, A;Murgo, A;Antognoli, A;Ianzano, ML;Stivani, V;Grosso, V;Iezzi, M;Stramucci, L;Barbieri, E;Lemoli, RM;Nanni, P;Lollini, PL; | Human responses against HER-2-positive cancer cells in human immune system-engrafted mice | British journal of cancer | 22929887 | ec, Bergisch Gladbach, Germany) to obtain highly purified cells (mean purity 93%±6%) as previously described (Rossi et al, 2007). For challenge experiments, CD34+ or CD133+ cells were purchased from AllCells (Emeryville, CA, USA). All the experiments | Full Paper |
2012 | Fukushima, H;Kawanabe, N;Murata, S;Ishihara, Y;Yanagita, T;Balam, TA;Yamashiro, T; | SSEA-4 is a marker of human deciduous periodontal ligament stem cells | Journal of dental research | 22895512 | Human bone marrow cells were purchased from AllCells (Emeryville, CA, USA) and were maintained in low-glucose DMEM supplemented with 10% HIFBS and penicillin/ streptomycin. Save | Full Paper |
2012 | Kim, J;Denu, RA;Dollar, BA;Escalante, LE;Kuether, JP;Callander, NS;Asimakopoulos, F;Hematti, P; | Macrophages and mesenchymal stromal cells support survival and proliferation of multiple myeloma cells | British journal of haematology | 22583117 | Bone marrow (BM) microenvironment provides crucial supports to normal and malignant hematopoiesis and several types of cells such as endothelial cells, osteoblasts and Save | Full Paper |
2012 | Cokic, VP;Bhattacharya, B;Beleslin-Cokic, BB;Noguchi, CT;Puri, RK;Schechter, AN; | JAK-STAT and AKT pathway-coupled genes in erythroid progenitor cells through ontogeny | Journal of translational medicine | 22676255 | mmunomagnetic selection with MACS cell isolation system (Miltenyi Biotec, Auburn, CA). Commercial FL-derived CD34+ cells (Cambrex Bio Science, Inc., Walkersville, MD), CB- and BM-derived CD34+ cells (AllCells LLC, Berkeley, CA) are also isolated by p | Full Paper |
2012 | Mao, M;Sudhahar, V;Ansenberger-Fricano, K;Fernandes, DC;Tanaka, LY;Fukai, T;Laurindo, FR;Mason, RP;Vasquez-Vivar, J;Minshall, RD;Stadler, K;Bonini, MG; | Nitroglycerin drives endothelial nitric oxide synthase activation via the phosphatidylinositol 3-kinase/protein kinase B pathway | Free radical biology & medicine | 22037515 | Human and bovine aortic endothelial cells (HAEC or BAEC; Allcells, Emeryville, CA, USA), human microvascular endothelial cells (HMEC), and primary mouse endothelial cells (MEC) were cultured at 37 °C, 5% CO2 in corresponding endothelial medium with s | Full Paper |
2012 | William, AD;Lee, AC;Poulsen, A;Goh, KC;Madan, B;Hart, S;Tan, E;Wang, H;Nagaraj, H;Chen, D;Lee, CP;Sun, ET;Jayaraman, R;Pasha, MK;Ethirajulu, K;Wood, JM;Dymock, BW; | Discovery of the macrocycle (9E)-15-(2-(pyrrolidin-1-yl)ethoxy)-7,12,25-trioxa-19,21,24-triaza-tetracyclo[18.3.1.1(2,5).1(14,18)]hexacosa-1(24),2,4,9,14(26),15,17,20,22-nonaene (SB1578), a potent inhibitor of janus kinase 2/fms-like tyrosine kinase-3 (JAK2/FLT3) for the treatment of rheumatoid arthritis | Journal of medicinal chemistry | 22339472 | These cells were cultured as described previously. (34) Human CD4+ T cells, isolated from peripheral blood mononuclear cells with 95% purity, were purchased from AllCells (Emeryville, CA) and maintained in X-VIVO 20 media (Lonza, Basel, Switzerland) | Full Paper |
2012 | Tran, CA;Torres-Coronado, M;Gardner, A;Gu, A;Vu, H;Rao, A;Cao, LF;Ahmed, A;Digiusto, D; | Optimized processing of growth factor mobilized peripheral blood CD34+ products by counterflow centrifugal elutriation | Stem cells translational medicine | 23197821 | HPC-A products were obtained from AllCells LLC (Emeryville, CA, http://www.allcells.com), Key Biologics LLC (Memphis, TN, http://www.keybiologics.com), or Progenitor Cell Therapy LLC (Mountain View, CA, http://www.pctcelltherapy.com). HPC-A products | Full Paper |
2012 | Rodriguez-Menocal, L;Salgado, M;Ford, D;Van Badiavas, E; | Stimulation of skin and wound fibroblast migration by mesenchymal stem cells derived from normal donors and chronic wound patients | Stem cells translational medicine | 23197781 | We isolated and expanded human MSCs from fresh whole bone marrow obtained from normal donors and chronic wound patients. Normal donor bone marrow was acquired from AllCells LLC (Emeryville, CA, http://www.allcells.com). Chronic wound patient bone mar | Full Paper |
2012 | Oskouei, BN;Lamirault, G;Joseph, C;Treuer, AV;Landa, S;Da Silva, J;Hatzistergos, K;Dauer, M;Balkan, W;McNiece, I;Hare, JM; | Increased potency of cardiac stem cells compared with bone marrow mesenchymal stem cells in cardiac repair | Stem cells translational medicine | 23197758 | Human bone marrow aspirates were obtained from AllCells LLC (Emeryville, CA, http://allcells.com). Low-density mononuclear cells were separated from human bone marrow aspirates and cultured as described (see supplemental online data). Adherent MSCs w | Full Paper |
2012 | Soler, R;Füllhase, C;Hanson, A;Campeau, L;Santos, C;Andersson, KE; | Stem cell therapy ameliorates bladder dysfunction in an animal model of Parkinson disease | The Journal of urology | 22341818 | Different cell based therapies have been tested, focusing on motor function. We evaluated the effect of human amniotic fluid stem cells and bone marrow derived mesenchymal stem cells (ALLCELLS, Emeryville, California) on bladder dysfunction in a rat | Full Paper |
2012 | Hirata, Y;Ikeda, K;Sudoh, M;Tokunaga, Y;Suzuki, A;Weng, L;Ohta, M;Tobita, Y;Okano, K;Ozeki, K;Kawasaki, K;Tsukuda, T;Katsume, A;Aoki, Y;Umehara, T;Sekiguchi, S;Toyoda, T;Shimotohno, K;Soga, T;Nishijima, M;Taguchi, R;Kohara, M; | Self-enhancement of hepatitis C virus replication by promotion of specific sphingolipid biosynthesis | PLoS pathogens | 22916015 | les containing 200 µg protein were assayed for SPT activity using [14C]-serine and palmitoyl-CoA (Sigma-Aldrich, St. Louis, MO, USA) as substrates. Proliferation assay Human peripheral blood cells (AllCells, Emeryville, CA, USA) were plated onto 96- | Full Paper |
2012 | Köllmer, M;Keskar, V;Hauk, TG;Collins, JM;Russell, B;Gemeinhart, RA; | Stem cell-derived extracellular matrix enables survival and multilineage differentiation within superporous hydrogels | Biomacromolecules | 22404228 | Human bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque PLUS solution and RosetteSep Save | Full Paper |
2012 | Sharma, SM;Fuchsluger, T;Ahmad, S;Katikireddy, KR;Armant, M;Dana, R;Jurkunas, UV; | Comparative analysis of human-derived feeder layers with 3T3 fibroblasts for the ex vivo expansion of human limbal and oral epithelium | Stem cell reviews and reports | 21964568 | Bone marrow samples (3-10 ml) were purchased from AllCells (Emeryville, CA) and plated in T175 flasks in growth media consisting of α-MEM (Lonza, Walkersville, MD) supplemented Save | Full Paper |
2012 | McNamara, LA;Ganesh, JA;Collins, KL; | Latent HIV-1 infection occurs in multiple subsets of hematopoietic progenitor cells and is reversed by NF-κB activation | Journal of virology | 22718820 | bone marrow (BM) was obtained commercially (AllCells Ltd.); mononuclear cells were purified Presorted CD133 + BM or CB cells were obtained commercially (AllCells Ltd.) and were Save | Full Paper |
2012 | Yu, X;McCarthy, PJ;Wang, Z;Gorlen, DA;Mertz, JE; | Shutoff of BZLF1 gene expression is necessary for immortalization of primary B cells by Epstein-Barr virus | Journal of virology | 22623769 | B-cell proliferation assay. Human primary blood B cells were purchased from AllCells (Emeryville, CA), infected with the indicated amount of virus in green Raji units (GRU)/cell, aliquoted at 1 × 104 cells per well in 96-well plates, incubated at 37° | Full Paper |
2012 | Nan, XW;Tang, GJ;Xu, JG;Peng, TS;Miao, LS;Xu, ZG;Bai, C;Yu, P;Gao, YL;Hao, BT; | Imaging manifestations of renal primary neuroectodermal tumors | Chinese medical journal | 23044335 | Pathologicaland jmm unohistochemicalfindingsshowed portionofthetum ornecrosedanduni—morp hologicaloval orround sm allcells with rosettes structure underlight mi croscope.Im Save | Full Paper |
2012 | Lin, W;Oh, SK;Choo, AB;George, AJ; | Activated T cells modulate immunosuppression by embryonic-and bone marrow-derived mesenchymal stromal cells through a feedback mechanism | Cytotherapy | 22136295 | Frozen peripheral blood mononuclear cells (PBMC) were purchased from Allcells (Emeryville, CA, USA). All isolated T-cell cultures were maintained in Roswell Park Memorial Institute (RPMI)-1640 supplemented with 10% FBS, 2 mm l-glutamine, 50 U/mL peni | Full Paper |
2012 | Akalin, A;Garrett-Bakelman, FE;Kormaksson, M;Busuttil, J;Zhang, L;Khrebtukova, I;Milne, TA;Huang, Y;Biswas, D;Hess, JL;Allis, CD;Roeder, RG;Valk, PJ;Löwenberg, B;Delwel, R;Fernandez, HF;Paietta, E;Tallman, MS;Schroth, GP;Mason, CE;Melnick, A;Figueroa, ME; | Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemia | PLoS genetics | 22737091 | kemia gene rearranged AML samples harboring t(9;11) (MLL1 and MLL2) and one additional AML sample (AML) were available for processing. Two normal CD34+ bone marrow control samples were purchased from AllCells, LLC (Emeryville, CA, USA). Institutional | Full Paper |
2012 | Chaudhry, P;Yang, X;Wagner, M;Jong, A;Wu, L; | Retinoid-regulated FGF8f secretion by osteoblasts bypasses retinoid stimuli to mediate granulocytic differentiation of myeloid leukemia cells | Molecular cancer therapeutics | 22135230 | Normal human primitive hematopoietic CD34 + cells were obtained from AllCells and CD34 cells, certified to be HIV- and Mycoplasma-free by AllCells, were cultured for maxi Save | Full Paper |
2012 | Budhraja, A;Gao, N;Zhang, Z;Son, YO;Cheng, S;Wang, X;Ding, S;Hitron, A;Chen, G;Luo, J;Shi, X; | Apigenin induces apoptosis in human leukemia cells and exhibits anti-leukemic activity in vivo | Molecular cancer therapeutics | 22084167 | U937, Jurkat, and HL60 human leukemia cells were obtained from American Type Culture Collection and grown in RPMI-1640 medium with 10% FBS. Normal peripheral blood mononuclear cells (NPBMNC) were obtained from AllCells and maintained in RPMI-1640 wit | Full Paper |
2012 | de Lichtervelde, L;Antal, CE;Boitano, AE;Wang, Y;Krastel, P;Petersen, F;Newton, AC;Cooke, MP;Schultz, PG; | Euphohelioscopin A is a PKC activator capable of inducing macrophage differentiation | Chemistry & biology | 22921066 | R & D Systems]) unless otherwise indicated. Human mPB CD34+ cells were purified from fresh human leukophoresed mobilized peripheral blood (AllCells) using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following manufacturer’s protocols | Full Paper |
2012 | Qu, X;Zhuang, G;Yu, L;Meng, G;Ferrara, N; | Induction of Bv8 expression by granulocyte colony-stimulating factor in CD11b+Gr1+ cells: key role of Stat3 signaling | The Journal of biological chemistry | 22528488 | Freshly isolated bone marrow cells from five different healthy donors were obtained from AllCells (Emeryville, CA). Red blood cells were lysed by adding 4 volumes of 1× buffered Save | Full Paper |
2012 | Rowe, DD;Leonardo, CC;Recio, JA;Collier, LA;Willing, AE;Pennypacker, KR; | Human umbilical cord blood cells protect oligodendrocytes from brain ischemia through Akt signal transduction | The Journal of biological chemistry | 22158864 | Human umbilical cord blood (HUCB) cells protect the brain against ischemic injury, yet the mechanism of protection remains unclear. Using both in vitro and in vivo paradigms, this Save | Full Paper |
2012 | Madan, B;Goh, KC;Hart, S;William, AD;Jayaraman, R;Ethirajulu, K;Dymock, BW;Wood, JM; | SB1578, a novel inhibitor of JAK2, FLT3, and c-Fms for the treatment of rheumatoid arthritis | Journal of immunology (Baltimore, Md. : 1950) | 22962687 | Cells CD4+ T cells isolated from human PBMCs, with 95% purity were obtained from AllCells (Emeryville, CA). The THP-1 cell line was purchased from American Type Culture Collection and maintained as recommended | Full Paper |
2012 | Liu, W;Yan, M;Liu, Y;McLeish, KR;Coleman, WG;Rodgers, GP; | Olfactomedin 4 inhibits cathepsin C-mediated protease activities, thereby modulating neutrophil killing of Staphylococcus aureus and Escherichia coli in mice | Journal of immunology (Baltimore, Md. : 1950) | 22844115 | Human fresh bone-marrow neutrophils were obtained from AllCells (Emeryville, CA). Mouse neutrophils were isolated from bone marrow. The femur was removed from mice, then Save | Full Paper |
2012 | Ohtsubo, S;Ishikawa, M;Kamei, N;Kijima, Y;Suzuki, O;Sunagawa, T;Higashi, Y;Masuda, H;Asahara, T;Ochi, M; | The therapeutic potential of ex vivo expanded CD133+ cells derived from human peripheral blood for peripheral nerve injuries | Journal of neurosurgery | 22880720 | We used granulocyte-colony stimulating factor-mobilized CD133+ cells derived from human peripheral blood (lot no. PCA1521B, AllCells) for these experiments. The fresh CD133+ cells were cultured with serum-free medium (StemSpan, Stem Cell Technologies | Full Paper |
2012 | Kadam, SK;Farmen, M;Brandt, JT; | Quantitative measurement of cell-free plasma DNA and applications for detecting tumor genetic variation and promoter methylation in a clinical setting | The Journal of molecular diagnostics : JMD | 22579630 | Commercially available pooled human plasma (AllCells, Emeryville, CA) was used to determine matrix effect and for DNA spike-in experiments. All blood samples from healthy controls Save | Full Paper |
2012 | Magga, J;Savchenko, E;Malm, T;Rolova, T;Pollari, E;Valonen, P;Lehtonen, Š;Jantunen, E;Aarnio, J;Lehenkari, P;Koistinaho, M;Muona, A;Koistinaho, J; | Production of monocytic cells from bone marrow stem cells: therapeutic usage in Alzheimer's disease | Journal of cellular and molecular medicine | 21777378 | Flt-3, 20 ng/ml IL-6, 20 ng/ml IL-3, in humidified atmosphere at 37°C in 5% CO2. Cells were cultivated and differentiated as described earlier. Human PB GCSF-mobilized CD34 cells were obtained from AllCells and cultivated similarly to BM-originated c | Full Paper |
2012 | Xia, Y;Wübbeler, JH;Qi, Q;Steinbüchel, A; | Employing a recombinant strain of Advenella mimigardefordensis for biotechnical production of Homopolythioesters from 3,3'-dithiodipropionic acid | Applied and environmental microbiology | 22344658 | AftertheirODhadreached500Klettunits,allcells were transferred into the same MS medium containing 7 g/liter glycerol plus 9 g/liter DTDP as a precursor. (A) Increase of OD (Klett unit) Save | Full Paper |
2012 | Pei, L;Choi, JH;Liu, J;Lee, EJ;McCarthy, B;Wilson, JM;Speir, E;Awan, F;Tae, H;Arthur, G;Schnabel, JL;Taylor, KH;Wang, X;Xu, D;Ding, HF;Munn, DH;Caldwell, C;Shi, H; | Genome-wide DNA methylation analysis reveals novel epigenetic changes in chronic lymphocytic leukemia | Epigenetics | 22534504 | CD19+/CD27+ memory B-cell, and CD19+/CD27- naïve B-cell samples used in the RRBS analysis were purchased from ALLCELLs Inc. Other normal CD19+ B-cell samples were purified from leuko-enriched blood samples purchased from a local blood bank. Mononucle | Full Paper |
2012 | Behbehani, GK;Bendall, SC;Clutter, MR;Fantl, WJ;Nolan, GP; | Single-cell mass cytometry adapted to measurements of the cell cycle | Cytometry. Part A : the journal of the International Society for Analytical Cytology | 22693166 | Mass cytometry is a recently introduced technology that utilizes transition element isotope‐tagged antibodies for protein detection on a single‐cell basis. By circumventing the limitations Save | Full Paper |
2012 | Kanagal-Shamanna, R;Zhao, W;Vadhan-Raj, S;Nguyen, MH;Fernandez, MH;Medeiros, LJ;Bueso-Ramos, CE; | Over-expression of CYP2E1 mRNA and protein: implications of xenobiotic induced damage in patients with de novo acute myeloid leukemia with inv(16)(p13.1q22); CBFβ-MYH11 | International journal of environmental research and public health | 23066397 | Island, NY, USA). cDNA synthesis was performed using the QuantiTect RT Kit (Qiagen, Valencia, CA, USA) according to manufacturer’s instructions [14]. The commercially produced normal BM CD34 cDNA (AllCells, LLC, Emeryville, CA, USA) was also used in | Full Paper |
2012 | Puri, N;Sodhi, K;Haarstad, M;Kim, DH;Bohinc, S;Foglio, E;Favero, G;Abraham, NG; | Heme induced oxidative stress attenuates sirtuin1 and enhances adipogenesis in mesenchymal stem cells and mouse pre-adipocytes | Journal of cellular biochemistry | 22234917 | For human MSCs, frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential Save | Full Paper |
2012 | Spencer, R; | Magnetic resonance in tissue engineering | NMR in biomedicine | 22134896 | Mesenchymal stem cells isolated from fresh adult human bone marrow were obtained commercially by AllCells (AllCells LLC, Berkeley, CA, USA). Nucleated cells were incubated in a Save | Full Paper |
2012 | Guo, LW;Wu, Q;Green, B;Nolen, G;Shi, L;Losurdo, J;Deng, H;Bauer, S;Fang, JL;Ning, B; | Cytotoxicity and inhibitory effects of low-concentration triclosan on adipogenic differentiation of human mesenchymal stem cells | Toxicology and applied pharmacology | 22726953 | Human adult mesenchymal stem cells (hMSCs) were originally provided by AllCells (Emeryville, CA). hMSCs were grown in alpha Minimum Essential Medium (αMEM), supplemented Save | Full Paper |
2012 | Xu, D;Kim, Y;Postelnek, J;Vu, MD;Hu, DQ;Liao, C;Bradshaw, M;Hsu, J;Zhang, J;Pashine, A;Srinivasan, D;Woods, J;Levin, A;O'Mahony, A;Owens, TD;Lou, Y;Hill, RJ;Narula, S;DeMartino, J;Fine, JS; | RN486, a selective Bruton's tyrosine kinase inhibitor, abrogates immune hypersensitivity responses and arthritis in rodents | The Journal of pharmacology and experimental therapeutics | 22228807 | One million human cord blood-derived CD34+ hematopoietic stem cells (AllCells, LLC, Emeryville, CA) were cultured in a serum-free StemPro-34 complete medium (Invitrogen) with supplements, stem cell factor (100 ng/ml), and IL-6 (50 ng/ml). IL-3 (10 ng | Full Paper |
2012 | Pirlo, RK;Wu, P;Liu, J;Ringeisen, B; | PLGA/hydrogel biopapers as a stackable substrate for printing HUVEC networks via BioLP | Biotechnology and bioengineering | 21830203 | Two major challenges in tissue engineering are mimicking the native cell-cell arrangements of tissues and maintaining viability of three‐dimension (3D) tissues thicker than 300 µm. Save | Full Paper |
2012 | Zou, C;Chou, BK;Dowey, SN;Tsang, K;Huang, X;Liu, CF;Smith, C;Yen, J;Mali, P;Zhang, YA;Cheng, L;Ye, Z; | Efficient derivation and genetic modifications of human pluripotent stem cells on engineered human feeder cell lines | Stem cells and development | 22225458 | Bone marrow-derived MSCs were established from CD34-depleted mononuclear cells (MNCs) (AllCells) as previously described [16], from adult donors (such as a male donor #BM2426). Primary MSCs and immortalized MSCs were cultured in Dulbecco's modified E | Full Paper |
2012 | Aslanidi, GV;Rivers, AE;Ortiz, L;Govindasamy, L;Ling, C;Jayandharan, GR;Zolotukhin, S;Agbandje-McKenna, M;Srivastava, A; | High-efficiency transduction of human monocyte-derived dendritic cells by capsid-modified recombinant AAV2 vectors | Vaccine | 22497875 | DMEM (Invitrogen) supplemented with 10% FBS (Sigma) and antibiotics (Lonza). Leukapheresis-derived peripheral blood mononuclear cells (PBMCs) (AllCells) were purified on Ficoll-Paque (GEHeathCare), resuspended in serum-free AIM-V medium (Lonza), and | Full Paper |
2012 | Koestler, DC;Marsit, CJ;Christensen, BC;Accomando, W;Langevin, SM;Houseman, EA;Nelson, HH;Karagas, MR;Wiencke, JK;Kelsey, KT; | Peripheral blood immune cell methylation profiles are associated with nonhematopoietic cancers | Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology | 22714737 | Sorted, normal, human, peripheral blood leukocyte subtypes were purchased from AllCells. Leukocytes were isolated from different, anonymous, nondiseased individuals' whole blood ( Save | Full Paper |
2012 | Hong, L;Wei, N;Joshi, V;Yu, Y;Kim, N;Krishnamachari, Y;Zhang, Q;Salem, AK; | Effects of glucocorticoid receptor small interfering RNA delivered using poly lactic-co-glycolic acid microparticles on proliferation and differentiation capabilities of human mesenchymal stromal cells | Tissue engineering. Part A | 21988716 | All other chemicals and solvents used, including dichloromethane (DCM) and acetonitrile, were of high-performance liquid chromatography analytical grade. Fresh human bone marrow was purchased from Allcells (Emeryville, CA). Invitrogen (Carlsbad, CA) | Full Paper |
2012 | Noto, PB;Bukhtiyarov, Y;Shi, M;McKeever, BM;McGeehan, GM;Lala, DS; | Regulation of sphingomyelin phosphodiesterase acid-like 3A gene (SMPDL3A) by liver X receptors | Molecular pharmacology | 22810003 | Whole blood from human donors was purchased from AllCells, LLC (Emeryville, CA). Human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood collected Save | Full Paper |
2012 | Abdolhosseini, M;Nandula, SR;Song, J;Hirt, H;Gorr, SU; | Lysine substitutions convert a bacterial-agglutinating peptide into a bactericidal peptide that retains anti-lipopolysaccharide activity and low hemolytic activity | Peptides | 22484285 | GL13NH2 is a bacteria-agglutinating peptide derived from the sequence of the salivary protein parotid secretory protein (PSP, BPIFA2, SPLUNC2, C20orf70). The peptide agglutinates Save | Full Paper |
2012 | Berezhnyy, IV;Berezhna, SY; | Fast multi-spectral imaging technique for detection of circulating endothelial cells in human blood samples | Journal of biomedical optics | 23224165 | Blood samples from healthy donors were purchased from AllCells, LLC., Emeryville, CA. The blood was collected via venipuncture in EDTA tubes, stored and delivered at 4°C. Before processing, a white blood cell count was performed using Scepter™ 2.0 ce | Full Paper |
2012 | Shah, SN;Kerr, C;Cope, L;Zambidis, E;Liu, C;Hillion, J;Belton, A;Huso, DL;Resar, LM; | HMGA1 reprograms somatic cells into pluripotent stem cells by inducing stem cell transcriptional networks | PloS one | 23166588 | tocol# MO11M279). All mice were housed in a sterile environment where they had free access to food and water as outlined in our institutional guidelines. Cell Culture MSC1640 mesenchymal stem cells (AllCells, LLC) were maintained in DMEM (low glucos | Full Paper |
2012 | Ringpis, GE;Shimizu, S;Arokium, H;Camba-Colón, J;Carroll, MV;Cortado, R;Xie, Y;Kim, PY;Sahakyan, A;Lowe, EL;Narukawa, M;Kandarian, FN;Burke, BP;Symonds, GP;An, DS;Chen, IS;Kamata, M; | Engineering HIV-1-resistant T-cells from short-hairpin RNA-expressing hematopoietic stem/progenitor cells in humanized BLT mice | PloS one | 23300932 | for 2 hours in the presence of 8 μg/ml of polybrene. Transductions of PBMCs were performed as previously described [23] with indicated cell numbers and MOIs. mPB-CD34+ cells (9.8×104), provided by AllCells (Emeryville, CA), were pre-stimulated with S | Full Paper |
2012 | Zhu, J;Siclari, VA;Liu, F;Spatz, JM;Chandra, A;Divieti Pajevic, P;Qin, L; | Amphiregulin-EGFR signaling mediates the migration of bone marrow mesenchymal progenitors toward PTH-stimulated osteoblasts and osteocytes | PloS one | 23300521 | Human bone marrow aspirates were purchased from Allcells and purified through Ficoll gradient (GE Healthcare) to obtain bone marrow mononuclear cells. These cells were seeded Save | Full Paper |
2012 | Wen, H;Li, Y;Malek, SN;Kim, YC;Xu, J;Chen, P;Xiao, F;Huang, X;Zhou, X;Xuan, Z;Mankala, S;Hou, G;Rowley, JD;Zhang, MQ;Wang, SM; | New fusion transcripts identified in normal karyotype acute myeloid leukemia | PloS one | 23251452 | ry samples with abnormal karyotype AML and eight AML primary samples without karyotype information [Figure 1], of which 38 were from University of Michigan Comprehensive Cancer Center and 7 were from Allcells Inc (AllCells, CA). The use of samples fr | Full Paper |
2012 | Kobayashi, DT;Decker, D;Zaworski, P;Klott, K;McGonigal, J;Ghazal, N;Sly, L;Chung, B;Vanderlugt, J;Chen, KS; | Evaluation of peripheral blood mononuclear cell processing and analysis for Survival Motor Neuron protein | PloS one | 23226377 | A: SMN signal was evaluated in lysates created from a single sample purchased from AllCells. PBMCs were processed in ER4 at densities of 10 6 , 10 7 and 5×10 7 cells/mL and the Save | Full Paper |
2012 | Zhang, LJ;Tao, BB;Wang, MJ;Jin, HM;Zhu, YC; | PI3K p110α isoform-dependent Rho GTPase Rac1 activation mediates H2S-promoted endothelial cell migration via actin cytoskeleton reorganization | PloS one | 22970259 | uman recombinant Rac1 and G-LISA Rac1 activation assay kits were obtained from Cytoskeleton (Denver, CO, USA). Cell culture-Primary human umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Emeryville, CA, USA). Each batch of HUVE | Full Paper |
2012 | Venet, S;Ravn, U;Buatois, V;Gueneau, F;Calloud, S;Kosco-Vilbois, M;Fischer, N; | Transferring the characteristics of naturally occurring and biased antibody repertoires to human antibody libraries by trapping CDRH3 sequences | PloS one | 22937053 | Human CDRH3 from three donors were amplified by nested PCR from commercially available peripheral blood purified cells cDNA (AllCells and BioChain). The first PCR allowed the Save | Full Paper |
2012 | Park, TS;Huo, JS;Peters, A;Talbot, CC;Verma, K;Zimmerlin, L;Kaplan, IM;Zambidis, ET; | Growth factor-activated stem cell circuits and stromal signals cooperatively accelerate non-integrated iPSC reprogramming of human myeloid progenitors | PloS one | 22905176 | For these experiments, highly purified (>96%) CD34 + CD45 + CB cells were received within 24 hours of neonatal harvest from AllCells (catalog number: CB005). On the same day (day Save | Full Paper |
2012 | Milanesi, A;Lee, JW;Li, Z;Da Sacco, S;Villani, V;Cervantes, V;Perin, L;Yu, JS; | β-Cell regeneration mediated by human bone marrow mesenchymal stem cells | PloS one | 22879915 | BMSCs from a normal adult donor were purchased from a commercial source AllCells, LLC (5858 Horton Street, Suite 360, Emeryville, California, 94608 USA; catalog number ABM005) Save | Full Paper |
2012 | Boomsma, RA;Geenen, DL; | Mesenchymal stem cells secrete multiple cytokines that promote angiogenesis and have contrasting effects on chemotaxis and apoptosis | PloS one | 22558198 | TCTCCTC GTCTGCCTCTTTTGGTCAGG Angiogenesis Assay The effect of paracrine factors secreted by MSC into CM on angiogenesis was studied using canine jugular vein vascular endothelial cells (CVEC; AllCells) and the Fibrin Gel In Vitro Angiogenesis | Full Paper |
2012 | Basile, LA;Ellefson, D;Gluzman-Poltorak, Z;Junes-Gill, K;Mar, V;Mendonca, S;Miller, JD;Tom, J;Trinh, A;Gallaher, TK; | HemaMax™, a recombinant human interleukin-12, is a potent mitigator of acute radiation injury in mice and non-human primates | PloS one | 22383962 | clear cells (PBMC) in vitro and the PK and PD characteristics of HemaMax in vivo. Isolation of CD14- PBMC and Quantification of IFN-γ Secretion Human PBMC collected by apheresis were purchased from AllCells (Emeryville, CA, USA). Mouse and rhesus mo | Full Paper |
2012 | Gao, N;Cheng, S;Budhraja, A;Liu, EH;Chen, J;Chen, D;Yang, Z;Luo, J;Shi, X;Zhang, Z; | 3,3'-Diindolylmethane exhibits antileukemic activity in vitro and in vivo through a Akt-dependent process | PloS one | 22363731 | lls were washed and assayed for total number and viability using trypan blue exclusion. Cells were suspended at 8×105/mL for treatment. Fresh normal bone marrow mononuclear cells were purchased from Allcells (Emeryvill, CA). After washing and enumera | Full Paper |
2012 | Rellick, SL;O'Leary, H;Piktel, D;Walton, C;Fortney, JE;Akers, SM;Martin, KH;Denvir, J;Boskovic, G;Primerano, DA;Vos, J;Bailey, N;Gencheva, M;Gibson, LF; | Bone marrow osteoblast damage by chemotherapeutic agents | PloS one | 22363485 | Mediatech), 0.05 µM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), non-essential amino acids and B-FGF solution. H9 cells were moved to HOB feeder layers where indicated. CD34+ bone marrow cells (ALLCELLS, Emeryville, CA) were grown in RPMI-1640 s | Full Paper |
2012 | Houseman, EA;Accomando, WP;Koestler, DC;Christensen, BC;Marsit, CJ;Nelson, HH;Wiencke, JK;Kelsey, KT; | DNA methylation arrays as surrogate measures of cell mixture distribution | BMC bioinformatics | 22568884 | ples, de-identified specimens that were not subject to human subjects review by an institutional review board (IRB). The sorted, normal, human, peripheral blood leukocyte subtypes were purchased from AllCellsⓇ, LLC (Emeryville, CA) and were isolated | Full Paper |
2012 | Christopherson, KW;Frank, RR;Jagan, S;Paganessi, LA;Gregory, SA;Fung, HC; | CD26 protease inhibition improves functional response of unfractionated cord blood, bone marrow, and mobilized peripheral blood cells to CXCL12/SDF-1 | Experimental hematology | 22846168 | Medical Center (RUMC) with institutional review board (IRB) approval. Human G-CSF mobilized peripheral blood (mobPB) was purchased from AllCells, LLC (Emeryville, CA, USA). Mononuclear cells (MNC) were isolated by density gradient centrifugation over | Full Paper |
2012 | Iancu-Rubin, C;Gajzer, D;Mosoyan, G;Feller, F;Mascarenhas, J;Hoffman, R; | Panobinostat (LBH589)-induced acetylation of tubulin impairs megakaryocyte maturation and platelet formation | Experimental hematology | 22381681 | Human MK were generated in liquid cultures from bone marrow (BM)-derived CD34+ cells (AllCells, Inc. Emeryville, CA). Cells were suspended in IMDM medium (Invitrogen, Grand Island, NY) supplemented with 1% penicillin/streptomycin, 1% L-Glutamine (Inv | Full Paper |
2012 | Fujioka, Y;Tanaka, N;Nakanishi, K;Kamei, N;Nakamae, T;Izumi, B;Ohta, R;Ochi, M; | Magnetic field-based delivery of human CD133⁺ cells promotes functional recovery after rat spinal cord injury | Spine | 22246536 | Phosphate-buffered saline (PBS) solution (50 μL) containing 1 × 10 5 of human peripheral blood-derived CD133 + cells (AllCells, Emeryville, CA) was injected into the subarachnoid Save | Full Paper |
2012 | Huovinen, P;Petersen, H; | Particlization in hybrid models | The European Physical Journal A | The first one that we call "allcells" sampling, contains a loop over all hypersurface elements The straightforward "allcells" sampling does not do it, and therefore we have developed the Save | Full Paper | |
2012 | Kusao, I;Shiramizu, B;Liang, CY;Grove, J;Agsalda, M;Troelstrup, D;Velasco, VN;Marshall, A;Whitenack, N;Shikuma, C;Valcour, V; | Cognitive performance related to HIV-1-infected monocytes | The Journal of neuropsychiatry and clinical neurosciences | 22450616 | assay for IL6, IL8, IL10, TNFα, sFAS, sTNFRI, sTNFRII, and MCP1. For the in-vitro experiments, monocytes were separated from commercially-available PBMC (AllCells; Emeryville, CA) using the EasySep Human CD14 Positive Selection Kit (Stem Cell Technol | Full Paper |
2012 | Yu, Y;Wei, N;Stanford, C;Schmidt, T;Hong, L; | In vitro effects of RU486 on proliferation and differentiation capabilities of human bone marrow mesenchymal stromal cells | Steroids | 22093480 | Fresh human bone marrows, donated by healthy donors from 21 to 47 years of age, were purchased from Allcells (Emeryville, CA). Mononuclear cells were isolated using density Save | Full Paper |
2012 | Tatsumi, K;Taatjes, DJ;Wadsworth, MP;Bouchard, BA;Bovill, EG; | Cell adhesion molecule 1 (CADM1) is ubiquitously present in the endothelium and smooth muscle cells of the human macro- and micro-vasculature | Histochemistry and cell biology | 22940845 | Immunostained cells were visualized with an Olympus BX51 fluorescence microscope (Olympus America, Center Valley, PA). For each experiment (n = 3) human umbilical vein ECs (AllCells, Emeryville CA), cultured as per the manufacturer’s directions, were | Full Paper |
2012 | He, M;Chen, P;Arnovitz, S;Li, Y;Huang, H;Neilly, MB;Wei, M;Rowley, JD;Chen, J;Li, Z; | Two isoforms of HOXA9 function differently but work synergistically in human MLL-rearranged leukemia | Blood cells, molecules & diseases | 22633751 | The mononuclear cell (MNC) samples were isolated from normal BM cells purchased from AllCells, LLC. MNC cells were isolated using NycoPrep 1.077A (Axis-Shield, Oslo, Norway) Save;in Dulbecco modified Eagle's medium (DMEM) supplemented with 10% FBS | Full Paper |
2012 | Kobayashi, M;Kawase, T;Horimizu, M;Okuda, K;Wolff, LF;Yoshie, H; | A proposed protocol for the standardized preparation of PRF membranes for clinical use | Biologicals : journal of the International Association of Biological Standardization | 22841724 | Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells, LLC (Emeryville, CA) and maintained in an endothelial cell medium that was fully supplemented with growth factors (HuMedia-EB2; KURABO, Osaka, Japan). For the scratch assay, | Full Paper |
2012 | Aoki, T;Miyazaki, K;Katayama, T;Watanabe, M;Horie, R;Danbara, M;Higashihara, M; | Surface CD3 expression proceeds through both myosin regulatory light chain 9 (MYL9)-dependent and MYL9-independent pathways in Jurkat cells | Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi | 23538510 | K562 cells were transfected using the 96-well Nucleofector Kit SF (Lonza) per manufacturer's recommendations. PBMCs (AllCells) were received on ice, centrifuged at 1,000g for 5 min at room temperature (22 °C) and changed to pre-warmed RPMI medium sup | Full Paper |
2012 | Wakao, S;Kitada, M;Kuroda, Y;Dezawa, M; | Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages | Methods in molecular biology (Clifton, N.J.) | 22167642 | candidate cell source for isolation of Muse cells. We recommend to use adult human dermal fibroblasts (CC-2511, Lonza, Basel, Switzerland; 2320, ScienCell, Carlsbad, CA) and adult human bone marrow stromal cells (PT-2501, Lonza; ABM001, AllCells, Eme | Full Paper |
2012 | Mendelson, A; | Chondrogenesis of Stem/Progenitor Cells by Chemotaxis Using Novel Cell Homing Systems | Thesis | Human bone marrow MSCs were isolated from fresh adult donor samples (AllCells, Emeryville, CA) using our prior methods [94, 96, 115]. Briefly, the bone marrow sample was Save | Full Paper | |
2012 | Balzano, G;Liesch, B; | The role of chroma and scalestep in the recognition of musical intervals in and out of context. | Psychomusicology: A Journal of Research in Music Cognition | The octave presented somewhat of a problem in these analyses; latencies (and errors) tended to be drastically lower in allcells ofthe matrix involving the octave, and because of the Save | Full Paper | |
2012 | Gratien, J; | Implementing Lowest-Order Methods for Diffusive Problems with a DSEL | Modelling and Simulation in Fluid Dynamics in Porous Media | The integration algorithm consists in iterating on the cell elements of the allCells(Th) and evaluating the bilinear expression on each cell. This bilinear expression is composed of: Save | Full Paper | |
2012 | YAN, LI; | Improvements on Humanized NOD/SCID lL-2rg-/-Mice | Thesis | Purified human CD133+ cord blood cells were purchased from AllCells (California). Alternatively, umbilical cord blood was obtained from the Singapore Cord Blood Bank. Red blood Save | Full Paper | |
2012 | Nikdel, A;Jameii, SM;Faez, K; | A novel area coverage protocol based on particle swarm optimization algorithm for wireless sensor networks | Global Journal on Technology | will be provided if the sum of cells which are covered (ie Sumcells set), are equal to the sum of cells which will be covered if all nodes have maximum sensing radius (ie Allcells set). As Save | Full Paper | |
2012 | Huff, RR; | Induction of chondrogenesis from MSCs by BMP7 and TGFB1 in low oxygen tension: expression of superficial zone protein | Thesis | The IRC received the sample as whole bone marrow, purchased commercially (AllCells Inc.). To isolate the MSCs, nucleated cells were recovered from a 25mL sample using a Ficoll- Save | Full Paper | |
2012 | Sunderic, K; | Effects of Mild Heating on the Osteogenic Differentiation of Human Mesenchymal Stem Cells during Inflammation | Thesis | Human bone marrow explanted from the iliac crest of a 24-year old donor was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the Save | Full Paper | |
2012 | Sangokoya, CO; | MicroRNA Function in Cellular Stress Response | Thesis | HbAA CD34+ cells (ALLCells) and HbSS CD34+ cells were purified from blood collected from apheresis with donor consent according to Duke IRB protocol. Mononuclear cells were separated by Ficoll-Hypaque density gradient, and CD34+ cells were enriched b | Full Paper | |
2012 | McNamara, LA; | HIV-1 Infection of Hematopoietic Progenitor Cells. | Thesis | We obtained pre-existing umbilical cord blood lacking subject identifiers after scheduled cesarean section procedures. We obtained fresh whole bone marrow aspirates from a commercial source (AllCells Ltd.). We prepared BMMCs and UCB mononuclear cells | Full Paper | |
2012 | Lowe, RM; | Investigation into the role of novel interferon lambdas in the treatment of viral disease, primarily hepatitis C | Thesis | +L-glutamine and 100ng/mL Macrophage colony-stimulating factor (M-CSF) and incubated for four days to differentiate into macrophages. Mast cells were derived from cord blood mononuclear cells (AllCells), cells were cultured in Iscove’s modified Dulbe | Full Paper | |
2012 | David, G; | Le locus 1q32: susceptibilité aux maladies inflammatoires de l'intestin et rôles biologiques de C1orf106 et KIF21B | Thesis | préparations d’ARN ont été conservées à -80°C. Les ARN des cellules primaires CD4+, CD8+, CD14+ et CD19+ proviennent de la compagnie Allcells (Californie, États-Unis) | Full Paper | |
2012 | Lin, X; | Novel Factors in Myeloproliferative Neoplasms-The Role of NFIB and miRNA Deregulation in Hematopoietic Cell Proliferation and Differentiation | Thesis | JAK2V617F mutation was detected in 7 out of 9 specimens by reverse transcription/PCR (AmpliTaq Gold) and directly sequencing JAK2 sequences of 1849 nucleotides amplified. Normal bone marrow CD34+ cells were purchased from AllCells. To isolate CD34+ c | Full Paper | |
2012 | Budhraja, A; | Molecular mechanism of apigenin induced apoptosis in leukemia | Thesis | Normalperipheral blood mononuclear cells were obtained from AllCells (Emeryville, CA, USA)and were maintained in RPMI 1640 supplemented with 10% FBS. Dr. Ruth Craig( Save | Full Paper | |
2012 | Severson, EBS; | Developing a Novel In-Vitro Neuroinflammation Model for Huntington's Disease | Thesis | Human BM aspirates were purchased from Lonza and AllCells. Density gradient centrifugation (Ficoll-Hypaque) was used to isolate mononuclear BM-MSC from three donors (AC1, Save | Full Paper | |
2012 | Mahmoud, AEM; | Investigating the biochemical activity of some flavonoids, coumarins and modified gold-nanoparticles as anti-breast-cancer agents | Thesis | Allcells were incubated for 2 days at 37 C under 5 % CO2. When the cells reached 50%confluent, the old media was removed and the adherent cells were first washed by IXversene. Save | Full Paper | |
2012 | Suh, SS; | A study of microRNAs associated with multiple myeloma pathogenesis and microRNAs/TP53 feedback circuit in human cancers, multiple myeloma and glioblastoma multiforme | Thesis | with monoclonal mouse anti-human CD138 antibodies using the AutoMACs automated separation system (Miltenyi-Biotec Three of the six healthy PCs were obtained from ALLCELLS, Save | Full Paper | |
2012 | Berk, E; | Dendritic cells regulate the induction of effector and memory CD8+ t cells | Thesis | SW480 and SW620 (ATCC, Manassas, VA) were grown in IMDM+10% FBS, while HUVECs (AllCells, Emeryville, CA) were cultured in HUVEC complete media (Basal media supplemented with HUVEC stimulatory supplement; AllCells). All were washed, reseeded at 20,000 | Full Paper | |
2012 | Peters, HLC; | Characteristics of amyloid precursor-like protein 2 (APLP2) in pancreatic cancer and Ewing's sarcoma | Thesis | Allcells and cellular debris were pelleted by centrifugation at 2,000 xg at 4C for 5 min. Thesupernatant was discarded and pellets containing 5 x 106 cells were resuspended in 100/jL Save | Full Paper | |
2012 | Chung, TY; | Experimental Beam Studies of Plasma-generated Species Interaction with Polymeric Materials and Biomolecules | Thesis | The basic protocol is shown in Figure 3.6. Heparinized fresh human whole blood was acquired from AllCells (Emeryville, CA, USA) and used within 6 h of collection. Samples were prepared by spotting serial diluted lipid A solution on UV/ozone-cleaned s | Full Paper | |
2012 | Hung, KC; | Developing peptide and antibody-mimetic ligands for the cell surface receptor beta2AR and DC-SIGN | Thesis | Sallusto and Lanzavecchia, 1994), except that hPBMCs were from HLA-A2+ patients (AllCells, LLC.). iDCs were treated with M3H4-18-HA, stained with anti-HA antibody, and analyzed by either flow cytometry or microscopy same as 293T cells, except that th | Full Paper | |
2012 | Simonds, EF; | Single-Cell Deep Profiling of Immune Signaling and Drug Responses in Normal and Malignant Human Hematopoiesis | Thesis | obtained from a tissue bank at Princess Margaret Hospital (Toronto, ON), courtesy of Dr. Mark D. Minden. Cryopreserved healthy BMMCs were purchased from AllCells, Inc. (Emeryville, CA). All human samples were obtained with informed consent in complia | Full Paper | |
2012 | Pardee, TS;Gomes, E;Jennings-Gee, J;Caudell, D;Gmeiner, WH; | Unique dual targeting of thymidylate synthase and topoisomerase1 by FdUMP[10] results in high efficacy against AML and low toxicity | Blood | 22362039 | Human cell lines, primary patient samples, or normal human HSCs were thawed and incubated in Stem SpanSFEM media supplemented with Stem Span CC100 cytokine cocktail (StemCell Technologies) for 24 hours with the indicated drug. Cells were then placed | Full Paper |
2012 | Zeiger, AS;Loe, FC;Li, R;Raghunath, M;Van Vliet, KJ; | Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behavior | PloS one | 22649562 | ganization correlate with an increase in proliferation and a decrease in motility of MSCs. Materials and Methods Cell Culture Human bone-marrow derived mesenchymal stromal or “stem” cells (MSCs; ReachBio Seattle, WA) were expanded until passage 4-6, | Full Paper |
2012 | Jolibois-Quinot, R; | Quantitative correlations among human mesenchymal stem cell mechanical properties and biological function | Thesis | Adult mesenchymal stem cells (referred later as aMSCs) were purchased from commercial sources: ReachBio human bone marrow mesenchymal stem cells - lot number 0090408 (referred later as RB1 - ReachBio LLC, Seattle, WA), and Poietics" human mesenchymal | Full Paper | |
2012 | Maloney, JM; | Chemomechanics of attached and suspended cells | Thesis | I have also been lucky to work with fine coauthors that dependably contributed honest and top-notch data and ideas: Christopher Bruce and Emily Walton (MIT), Franziska Lautenschläger and Jochen Guck (University of Cambridge), Dessy Nikova (MIT), Emer | Full Paper | |
2011 | Xu, B;Roos, JL;Dexheimer, P;Boone, B;Plummer, B;Levy, S;Gogos, JA;Karayiorgou, M; | Exome sequencing supports a de novo mutational paradigm for schizophrenia | Nat. Genet. | 21822266 | BX, JAG and MK designed the study, interpreted the data and prepared the manuscript; BX developed the analysis pipeline and had the primary role in analysis and validation of sequence data; JLR collected the samples and was the primary clinician on t | Full Paper |
2011 | Wood, AM;Van Bortle, K;Ramos, E;Takenaka, N;Rohrbaugh, M;Jones, BC;Jones, KC;Corces, VG; | Regulation of chromatin organization and inducible gene expression by a Drosophila insulator | Mol. Cell | 21981916 | Chromatin immunoprecipitation was performed as described (Bushey et al., 2009). To generate sequencing libraries ChIP DNA was prepared for adaptor ligation by end repair (‘End-It DNA End Repair Kit’ - Epicentre Cat# ER0720) and addition of ‘A’ base t | Full Paper |
2011 | Jänne, PA;Boss, DS;Camidge, DR;Britten, CD;Engelman, JA;Garon, EB;Guo, F;Wong, S;Liang, J;Letrent, S;Millham, R;Taylor, I;Eckhardt, SG;Schellens, JH; | Phase I dose-escalation study of the pan-HER inhibitor, PF299804, in patients with advanced malignant solid tumors | Clin. Cancer Res. | 21220471 | Immunohistochemistry analysis was performed by QualTek (Newtown, PA) and was automated throughout using a TechMate 500 or TechMate 1000 (BioTek Solutions/Ventana Medical Systems). Antibodies used were: pERK 1/2 clone 20G11 (Cell Signaling Cat # 4376) | Full Paper |
2011 | Ahmad, I;Morton, JP;Singh, LB;Radulescu, SM; | β-Catenin activation synergizes with PTEN loss to cause bladder cancer formation | Oncogene | invasive bladder carcinoma. We next looked at human bladder UCC using a tissue microarray of 80 cases, 60 UCC (transitional cell carcinomas) and 20 benign controls (Folio Biosciences, Columbus, OH, USA). Using the histoscore | Full Paper | |
2011 | Teichert, AE;Elalieh, H;Elias, PM;Welsh, J;Bikle, DD; | Overexpression of hedgehog signaling is associated with epidermal tumor formation in vitamin D receptor-null mice | J. Invest. Dermatol. | 21814234 | ffinity-purified, biotinylated goat anti-rabbit IgG, followed by ABC-peroxidase reagent, both purchased from Vector (Burlingame, CA). Peroxidase activity was detected with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followed | Full Paper |
2011 | Kelly, RK;Olson, DL;Sun, Y;Wen, D;Wortham, KA;Antognetti, G;Cheung, AE;Orozco, OE;Yang, L;Bailly, V;Sanicola, M; | An antibody-cytotoxic conjugate, BIIB015, is a new targeted therapy for Cripto positive tumours | Eur. J. Cancer | 21458984 | 2.3. Immunohistochemistry. Human breast, colon, lung and ovarian formalin-fixed paraffin-embedded tumour tissue arrays (Imgenex, San Diego, CA, Folio Biosciences, Columbus, OH and Zymed, San Francisco, CA) were stained as described previously | Full Paper |
2011 | Ahmad, I;Singh, LB;Foth, M;Morris, CA;Taketo, MM;Wu, XR;Leung, HY;Sansom, OJ;Iwata, T; | K-Ras and β-catenin mutations cooperate with Fgfr3 mutations in mice to promote tumorigenesis in the skin and lung, but not in the bladder | Dis Model Mech | 21504907 | Finally, we have examined the ERK-MAPK signaling pathway in human UCC by using a tissue microarray (TMA) that contains 60 UCC and 20 benign controls (Folio Biosciences, OH). Using the Histoscore technique, we have quantified the staining intensities | Full Paper |
2011 | Rokkam, D;Lafemina, MJ;Lee, JW;Matthay, MA;Frank, JA; | Claudin-4 levels are associated with intact alveolar fluid clearance in human lungs | Am. J. Pathol. | 21741940 | controls. Additional normal lung tissue specimens (n = 3) were also obtained (US Biomax, Rockville, MD; Folio Biosciences, Columbus, OH) and immunostained for claudin-4, as previously described. Histology Scoring. The | Full Paper |
2011 | Kent, BN;Salichos, L;Gibbons, JG;Rokas, A;Newton, IL;Clark, ME;Bordenstein, SR; | Complete bacteriophage transfer in a bacterial endosymbiont (Wolbachia) determined by targeted genome capture | Genome Biol Evol | 21292630 | Genomic DNA was extracted from ∼200 abdomens of the B-Wolbachia infected N. vitripennis 4.9 using the QIAGEN DNeasy Kit insect tissue protocol. DNA was treated with 100 mg/ml RNaseA and incubated at room temperature for 2 min. The resulting DNA was q | Full Paper |
2011 | Jiang, Y;Lu, J;Peatman, E;Kucuktas, H;Liu, S;Wang, S;Sun, F;Liu, Z; | A pilot study for channel catfish whole genome sequencing and de novo assembly | BMC Genomics | 22192763 | The culture supernatant was decanted and the block was inverted and tapped gently on paper towels to remove remaining liquid. BAC DNA was isolated using the Perfectprep™ BAC 96 kit (Eppendorf North America, Westbury, NY) according to the manufacturer | Full Paper |
2011 | Pitts, RJ;Rinker, DC;Jones, PL;Rokas, A;Zwiebel, LJ; | Transcriptome profiling of chemosensory appendages in the malaria vector Anopheles gambiae reveals tissue- and sex-specific signatures of odor coding | BMC Genomics | 21619637 | We thank the following individuals: Zhen Li for mosquito rearing; Travis Clark, Chelsea Baker and the Vanderbilt Genomics Shared Resource for sample preparation and Illumina sequencing; Shawn Levy and the Genomic Services Lab at HudsonAlpha Institute | Full Paper |
2011 | Liu, S;Zhou, Z;Lu, J;Sun, F;Wang, S;Liu, H;Jiang, Y;Kucuktas, H;Kaltenboeck, L;Peatman, E;Liu, Z; | Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP array | BMC Genomics | 21255432 | ;... sing the RNeasy plus Mini Kit (Qiagen, Valencia, CA, USA) with DNase I (Invitrogen, USA) treatment following the manufacturer's protocol. Illumina sequencing Sequencing was conducted commercially in HudsonAlpha Genomic Services Lab (Huntsville, | Full Paper |
2011 | Weiss, L;Bernstein, S;Jones, R;Amunugama, R;Krizman, D;Jebailey, L;Almogi-Hazan, O;Hazan, O;Yekhtin, Z;Yachtin, J;Shiner, R;Reibstein, I;Triche, E;Slavin, S;Or, R;Barnea, ER; | Preimplantation factor (PIF) analog prevents type I diabetes mellitus (TIDM) development by preserving pancreatic function in NOD mice | Endocrine | 21424847 | Department of Bone Marrow Transplantation and Cancer Immunotherapy, Hadassah University Hospital Ein Kerem, Hebrew University, Jerusalem, Israel Lola Weiss, Osnat Hazan, Janna Yachtin, Reut Shiner, Israel Reibstein & Reuven Or QualTek Molecular Labor | Full Paper |
2011 | Pawar, H;Kashyap, MK;Sahasrabuddhe, NA;Renuse, S;Harsha, HC;Kumar, P;Sharma, J;Kandasamy, K;Marimuthu, A;Nair, B;Rajagopalan, S;Maharudraiah, J;Premalatha, CS;Kumar, KV;Vijayakumar, M;Chaerkady, R;Prasad, TS;Kumar, RV;Kumar, RV;Pandey, A; | Quantitative tissue proteomics of esophageal squamous cell carcinoma for novel biomarker discovery | Cancer Biol. Ther. | 21743296 | The ESCCs varied from tumor grade I to grade III from patients in the age group of 39-79 y. A second group of tissue microarrays obtained from FolioBio (Columbus, OH) consisted of 40 ESCC tissues with matched adjacent normal esophageal squamous epith | Full Paper |
2011 | Bunting, RA;Duffy, KE;Lamb, RJ;San Mateo, LR;Smalley, K;Raymond, H;Liu, X;Petley, T;Fisher, J;Beck, H;Flavell, RA;Alexopoulou, L;Ward, CK; | Novel antagonist antibody to TLR3 blocks poly(I:C)-induced inflammation in vivo and in vitro | Cell. Immunol. | 21092943 | Mouse tissues (pancreas, inguinal and mesenteric lymph node, brain, liver, heart, trachea, colon, kidneys, lungs, and spleen) from three C57BL/6 and three TLR3KO mice were fixed in 10% neutral buffered formalin and processed for immunohistochemistry | Full Paper |
2011 | Trejos, AL;Jayaraman, S;Patel, RV;Naish, MD;Schlachta, CM; | Force sensing in natural orifice transluminal endoscopic surgery | Surg Endosc | 20559663 | allowing the flexible shaft to bend freely. The shaft and the wires were covered with heat-shrink thin-wall tubing (0.26 mm wall thickness, model Q-150K 1/8-02-QB481N- 25; Qualtek Electronics). The 2.8-mm instruments with the | Full Paper |
2011 | Karkera, J;Steiner, H;Li, W;Skradski, V;Moser, PL;Riethdorf, S;Reddy, M;Puchalski, T;Safer, K;Prabhakar, U;Pantel, K;Qi, M;Culig, Z; | The anti-interleukin-6 antibody siltuximab down-regulates genes implicated in tumorigenesis in prostate cancer patients from a phase I study | Prostate | 21321981 | PSA, IL‐6R, alpha‐methylacyl‐CoA racemase (AMACR), AR, and Chromogranin A were tested in tumor tissue samples at Qualtek Laboratories (Goleta, CA). Steam heat‐induced epitope retrieval (SHIER) method was used and immunohistochemical procedure was per | Full Paper |
2011 | Lynch, FC; | A method for following patients with retrievable inferior vena cava filters: results and lessons learned from the first 1,100 patients | J Vasc Interv Radiol | 21903414 | Full Paper | |
2011 | Shaw, CM;Scorza, LB;Waybill, PN;Singh, H;Lynch, FC; | Optional vena cava filter use in the elderly population | J Vasc Interv Radiol | 21530308 | Full Paper | |
2011 | Lynch, FC; | Removal of a Günther Tulip filter after 3,006 days | J Vasc Interv Radiol | 21277802 | Full Paper | |
2011 | Maxwell, SA;Cherry, EM;Bayless, KJ; | Akt, 14-3-3ζ, and vimentin mediate a drug-resistant invasive phenotype in diffuse large B-cell lymphoma | Leuk. Lymphoma | 21323512 | Preparation of DLBCL tissue extracts for Western blotting Normal and DLBCL lymphatic tissues were pro- cured through Folio Biosciences (Foliobio.com). Frozen tissue was ground to a powder with a pestle in a mortar while immersed in liquid nitrogen | Full Paper |
2011 | Farris, MH;Duffy, C;Findlay, RH;Olson, JB; | Streptomyces scopuliridis sp. nov., a bacteriocin-producing soil streptomycete | Int. J. Syst. Evol. Microbiol. | 20870885 | ; sequencing facility. Genomic DNA isolated from strain RB72 T using the method of Bollet et al. (1991) was sent to the HudsonAlpha Genomic Services Lab (Huntsville, AL) for Illumina Genome Analyser IIx sequencing. 16S rRNA | Full Paper |
2011 | Lee, LF;Axtell, R;Tu, GH;Logronio, K;Dilley, J;Yu, J;Rickert, M;Han, B;Evering, W;Walker, MG;Shi, J;de Jong, BA;Killestein, J;Polman, CH;Steinman, L;Lin, JC; | IL-7 promotes T(H)1 development and serum IL-7 predicts clinical response to interferon-β in multiple sclerosis | Science translational medicine | 21795588 | Human mononuclear cells were prepared from the buffy coats of samples from healthy adult donors (Stanford Blood Center) or from cord blood purchased from AllCells on Ficoll-Paque gradients. CD4+ T cells were isolated by magnetic bead depletion of CD1 | Full Paper |
2011 | Topp, MS;Kufer, P;Gökbuget, N;Goebeler, M;Klinger, M;Neumann, S;Horst, HA;Raff, T;Viardot, A;Schmid, M;Stelljes, M;Schaich, M;Degenhard, E;Köhne-Volland, R;Brüggemann, M;Ottmann, O;Pfeifer, H;Burmeister, T;Nagorsen, D;Schmidt, M;Lutterbuese, R;Reinhardt, C;Baeuerle, PA;Kneba, M;Einsele, H;Riethmüller, G;Hoelzer, D;Zugmaier, G;Bargou, RC; | Targeted therapy with the T-cell-engaging antibody blinatumomab of chemotherapy-refractory minimal residual disease in B-lineage acute lymphoblastic leukemia patients results in high response rate and prolonged leukemia-free survival | Journal of clinical oncology : official journal of the American Society of Clinical Oncology | 21576633 | Shown is the analysis of DNA fragmentation in B-lineage ALLcells after 48 hours of culture in serum-free medium alone. In this DNA fragmentation assay, intact chromatin and Save | Full Paper |
2011 | Yoneda, A;Kurokawa, T; | A sensitive sandwich ELISA to measure (1→ 3)-β-d-glucan levels in blood | Journal of immunological methods | 2009). 2.7. Measurement of plasma β-glucan. For the measurements of β-glucan level in human plasma, we used commercially available β-glucan-positive human plasma samples purchased from Discovery Life Sciences, Inc | Full Paper | |
2011 | Mayer, JA;Pham, T;Wong, KL;Scoggin, J;Sales, EV; | FISH-based determination of HER2 status in circulating tumor cells isolated with the microfluidic CEE™ platform | Cancer genetics | Peripheral blood was collected under appropriate third-party institutional review board-approved protocols (ConversantBIO, Huntsville, AL; BioOptions, Fullerton, CA) and delivered to Biocept's CLIA/CAP accredited laboratory | Full Paper | |
2011 | Lundgren, JA;Matsushima, K;Lynch, FC;Frankel, H;Cooney, RN; | Angiographic embolization of nonvariceal upper gastrointestinal bleeding: predictors of clinical failure | J Trauma | 21610434 | Full Paper | |
2011 | Balazs, AB;Chen, J;Hong, CM;Rao, DS;Yang, L;Baltimore, D; | Antibody-based protection against HIV infection by vectored immunoprophylaxis | Nature | 22139420 | Human peripheral mononuclear blood cells (AllCells) were thawed from −80 C, expanded in RPMI medium supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin mix( Save | Full Paper |
2011 | Sigal, A;Kim, JT;Balazs, AB;Dekel, E;Mayo, A;Milo, R;Baltimore, D; | Cell-to-cell spread of HIV permits ongoing replication despite antiretroviral therapy | Nature | 21849975 | Anonymous PBMCs or peripheral blood samples were provided by AllCells (PBMCs) or the UCLA Center for AIDS Research (CFAR) Virology Core Lab (peripheral blood). For whole blood, PBMCs were purified by Ficoll gradient using standard techniques. Purifie | Full Paper |
2011 | Doyon, Y;Vo, TD;Mendel, MC;Greenberg, SG;Wang, J;Xia, DF;Miller, JC;Urnov, FD;Gregory, PD;Holmes, MC; | Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures | Nature methods | 21131970 | K562 cells were transfected using the 96-well Nucleofector Kit SF (Lonza) per manufacturer's recommendations. PBMCs (AllCells) were received on ice, centrifuged at 1,000g for 5 min at room temperature (22 °C) and changed to pre-warmed RPMI medium sup | Full Paper |
2011 | Chou, BK;Mali, P;Huang, X;Ye, Z;Dowey, SN;Resar, LM;Zou, C;Zhang, YA;Tong, J;Cheng, L; | Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures | Cell research | 21243013 | f Hematology in Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA 5Cell Therapy Center, Xuanwu Hospital and Capital Medical University, Beijing, China 6AllCells LLC, Emeryville, California, USA *The Joh | Full Paper |
2011 | Lu, SJ;Li, F;Yin, H;Feng, Q;Kimbrel, EA;Hahm, E;Thon, JN;Wang, W;Italiano, JE;Cho, J;Lanza, R; | Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living mice | Cell research | 21221130 | versity of Illinois-Chicago review board. Informed consent was provided according to the Declaration of Helsinki. In some experiments, human blood samples were also obtained from a commercial source (AllCells, Emeryville, CA, USA). For hESC-PLTs, cul | Full Paper |
2011 | Kikushige, Y;Ishikawa, F;Miyamoto, T;Shima, T;Urata, S;Yoshimoto, G;Mori, Y;Iino, T;Yamauchi, T;Eto, T;Niiro, H;Iwasaki, H;Takenaka, K;Akashi, K; | Self-renewing hematopoietic stem cell is the primary target in pathogenesis of human chronic lymphocytic leukemia | Cancer cell | 21840488 | Human age-matched adult bone marrow and peripheral blood cells were obtained from healthy donors or purchased from AllCells Inc. (Emeryville, CA, USA). Informed consent was obtained from all patients and controls in accordance with the Helsinki Decla | Full Paper |
2011 | Panuganti, S; | Towards large-scale production of platelets for transfusion based on ex vivo expansion of megakaryocytes from hematopoietic stem cells | Thesis | Cultures were initiated in T-flasks with previously frozen mPB CD34-selected cells (AllCells; Berkeley, CA or Fred Hutchinson Cancer Research Center; Seattle, WA) and maintained at a concentration of 100,000 to 300,000 cells/ml in X-VIVO 20 (Lonza; W | Full Paper | |
2011 | Carter, CC;McNamara, LA;Onafuwa-Nuga, A;Shackleton, M;Riddell, J;Bixby, D;Savona, MR;Morrison, SJ;Collins, KL; | HIV-1 utilizes the CXCR4 chemokine receptor to infect multipotent hematopoietic stem and progenitor cells | Cell host & microbe | 21402361 | We obtained pre-existing umbilical cord blood lacking subject identifiers after scheduled cesarean section procedures. We obtained fresh whole bone marrow aspirates from a commercial source (AllCells Ltd.). We prepared BMMCs and UCB mononuclear cells | Full Paper |
2011 | Matsuyama, H;Suzuki, HI;Nishimori, H;Noguchi, M;Yao, T;Komatsu, N;Mano, H;Sugimoto, K;Miyazono, K; | miR-135b mediates NPM-ALK-driven oncogenicity and renders IL-17-producing immunophenotype to anaplastic large cell lymphoma | Blood | 22042699 | Many transformed lymphoma cells show immune-phenotypes resembling the corresponding normal lymphocytes, which gives a guide for proper diagnosis and presents promising Save;Human normal peripheral blood pan T lymphocytes were purchased from AllCell | Full Paper |
2011 | Aguila, JR;Liao, W;Yang, J;Avila, C;Hagag, N;Senzel, L;Ma, Y; | SALL4 is a robust stimulator for the expansion of hematopoietic stem cells | Blood | 21602528 | Normal human bone marrow CD34 + cells were purchased (AllCells) and single-cell suspensions were generated in StemSpan SFEM (StemCell Technologies) for 24 hours. Next, 10 5 Save | Full Paper |
2011 | Iancu-Rubin, C;Gajzer, D;Tripodi, J;Najfeld, V;Gordon, RE;Hoffman, R;Atweh, GF; | Down-regulation of stathmin expression is required for megakaryocyte maturation and platelet production | Blood | 21364187 | Human MKs were generated in liquid culture from peripheral blood (PB)-derived CD34 + cells purchased from AllCells and suspended in Iscove modified Dulbecco medium (Invitrogen) Save | Full Paper |
2011 | Liu, ZJ;Italiano, J;Ferrer-Marin, F;Gutti, R;Bailey, M;Poterjoy, B;Rimsza, L;Sola-Visner, M; | Developmental differences in megakaryocytopoiesis are associated with up-regulated TPO signaling through mTOR and elevated GATA-1 levels in neonatal megakaryocytes | Blood | 21304100 | Human umbilical CB-CD34+ cells were purchased from AllCells. G-CSF-mobilized adult peripheral blood (PB) CD34+ cells were purchased from the Fred Hutchinson Cancer Institute (Seattle, WA). CD34+ cells from either source were cultured in serum-free me | Full Paper |
2011 | Adams, GN;LaRusch, GA;Stavrou, E;Zhou, Y;Nieman, MT;Jacobs, GH;Cui, Y;Lu, Y;Jain, MK;Mahdi, F;Shariat-Madar, Z;Okada, Y;D'Alecy, LG;Schmaier, AH; | Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosis | Blood | 21297000 | HUVECs were purchased from AllCells and grown on gelatin-coated plates in CSC media (Cells Systems). Cells were grown to near-confluence in 6-well plates and transfected with Lipofectamine 2000 reagent (Invitrogen) in OPTI-MEM media (Invitrogen) over | Full Paper |
2011 | Hu, K;Yu, J;Suknuntha, K;Tian, S;Montgomery, K;Choi, KD;Stewart, R;Thomson, JA;Slukvin, II; | Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cells | Blood | 21296996 | Frozen CB mononuclear cells were obtained from AllCells. BM mononuclear cells from normal donors and from a patient with CML in the chronic phase were purchased from AllCells. Save | Full Paper |
2011 | Karp, JE;Smith, BD;Resar, LS;Greer, JM;Blackford, A;Zhao, M;Moton-Nelson, D;Alino, K;Levis, MJ;Gore, SD;Joseph, B;Carraway, H;McDevitt, MA;Bagain, L;Mackey, K;Briel, J;Doyle, LA;Wright, JJ;Rudek, MA; | Phase 1 and pharmacokinetic study of bolus-infusion flavopiridol followed by cytosine arabinoside and mitoxantrone for acute leukemias | Blood | 21239698 | Flavopiridol is a protein bound, cytotoxic, cyclin-dependent kinase inhibitor. Flavopiridol given by 1-hour bolus at 50 mg/m 2 daily 3 times followed by cytosine arabinoside and Save | Full Paper |
2011 | Huang, X;Gschweng, E;Van Handel, B;Cheng, D;Mikkola, HK;Witte, ON; | Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cells | Blood | 21163928 | The analyzed samples include H1 hESCs, day 7 and 15 H1 EB cells, and a mixture of 3 adult bone marrow CD34 + cells (AllCells). The data were analyzed with SDS RQ Manager 1.2 ( Save | Full Paper |
2011 | Lugthart, S;Figueroa, ME;Bindels, E;Skrabanek, L;Valk, PJ;Li, Y;Meyer, S;Erpelinck-Verschueren, C;Greally, J;Löwenberg, B;Melnick, A;Delwel, R; | Aberrant DNA hypermethylation signature in acute myeloid leukemia directed by EVI1 | Blood | 20855866 | healthy donors, 4 of which were acquired from the Translational Trials Development and Support Laboratory, Cincinnati Children's Hospital, and 4 of which were purchased from Allcells Save | Full Paper |
2011 | Mack, AA;Kroboth, S;Rajesh, D;Wang, WB; | Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectors | PloS one | 22132178 | a permit number or approval ID since mouse is not a protected species. Processing whole blood samples Peripheral (PB.1 and PB.2) and cord (CB.1 and CB.2) blood-derived CD34+ cells were obtained from AllCells (Emeryville, CA USA). Blood collections w | Full Paper |
2011 | Moorefield, EC;McKee, EE;Solchaga, L;Orlando, G;Yoo, JJ;Walker, S;Furth, ME;Bishop, CE; | Cloned, CD117 selected human amniotic fluid stem cells are capable of modulating the immune response | PloS one | 22046303 | lates for Elispot (Millipore, Billerica, MA) were coated with human interferon-γ (IFN-γ) capture antibody (2G1; Endogen, Rockford, IL). Peripheral blood mononuclear cells (PBMCs) were acquired from AllCells (Emeryville, CA), activated with 5 µg/ml PH | Full Paper |
2011 | Corbin, AS;Agarwal, A;Loriaux, M;Cortes, J;Deininger, MW;Druker, BJ; | Human chronic myeloid leukemia stem cells are insensitive to imatinib despite inhibition of BCR-ABL activity | The Journal of clinical investigation | 21157039 | Imatinib therapy, which targets the oncogene product BCR-ABL, has transformed chronic myeloid leukemia (CML) from a life-threatening disease into a chronic condition. Most patients, Save | Full Paper |
2011 | Rowe, DD; | Secreted factors from human umbilical cord blood cells protect oligodendrocytes from ischemic insult | Thesis | that prevented OL-HUCB cell contact but was permeable to media and soluble factors. Cryopreserved HUCB cells (ALLCELLS, Emeryville, CA) were rapidly thawed, washed, pelleted to remove the cryopreservatives and re-suspended in 10 ml DMEM with glucose | Full Paper | |
2011 | Zhou, L;McMahon, C;Bhagat, T;Alencar, C;Yu, Y;Fazzari, M;Sohal, D;Heuck, C;Gundabolu, K;Ng, C;Mo, Y;Shen, W;Wickrema, A;Kong, G;Friedman, E;Sokol, L;Mantzaris, I;Mantzaris, G;Pellagatti, A;Boultwood, J;Platanias, LC;Steidl, U;Yan, L;Yingling, JM;Lahn, MM;List, A;Bitzer, M;Verma, A; | Reduced SMAD7 leads to overactivation of TGF-beta signaling in MDS that can be reversed by a specific inhibitor of TGF-beta receptor I kinase | Cancer research | 21189329 | Bone marrow CD34+ cells from various normal donors were also obtained from AllCells (Emeryville, CA). K562 and HS-5 cell lines were purchased from ATCC. Erythropoietin and Transforming growth factor-β1 (TGF-β1) were bought from R&D Systems (Minneapol | Full Paper |
2011 | Nicolaides, TP;Li, H;Solomon, DA;Hariono, S;Hashizume, R;Barkovich, K;Baker, SJ;Paugh, BS;Jones, C;Forshew, T;Hindley, GF;Hodgson, JG;Kim, JS;Rowitch, DH;Weiss, WA;Waldman, TA;James, CD; | Targeted therapy for BRAFV600E malignant astrocytoma | Clinical cancer research : an official journal of the American Association for Cancer Research | 22038996 | Normal human astrocytes (NHA) were obtained from Clonetics and AllCells. All cell sources were authenticated through DNA fingerprinting using the Promega Powerplex platform. Save | Full Paper |
2011 | Kurtzberg, LS;Roth, S;Krumbholz, R;Crawford, J;Bormann, C;Dunham, S;Yao, M;Rouleau, C;Bagley, RG;Yu, XJ;Wang, F;Schmid, SM;Lavoie, EJ;Teicher, BA; | Genz-644282, a novel non-camptothecin topoisomerase I inhibitor for cancer treatment | Clinical cancer research : an official journal of the American Association for Cancer Research | 21415217 | Viable human bone marrow cells were purchased as frozen vials from AllCells, LLC., via StemCell Technologies (#ABM009). They were prepared with a DNase treatment (#07900; Save | Full Paper |
2011 | Arastu-Kapur, S;Anderl, JL;Kraus, M;Parlati, F;Shenk, KD;Lee, SJ;Muchamuel, T;Bennett, MK;Driessen, C;Ball, AJ;Kirk, CJ; | Nonproteasomal targets of the proteasome inhibitors bortezomib and carfilzomib: a link to clinical adverse events | Clinical cancer research : an official journal of the American Association for Cancer Research | 21364033 | Purpose: Bortezomib (Velcade), a dipeptide boronate 20S proteasome inhibitor and an approved treatment option for multiple myeloma, is associated with a treatment-emergent, painful Save | Full Paper |
2011 | Chao, MP;Alizadeh, AA;Tang, C;Jan, M;Weissman-Tsukamoto, R;Zhao, F;Park, CY;Weissman, IL;Majeti, R; | Therapeutic antibody targeting of CD47 eliminates human acute lymphoblastic leukemia | Cancer research | 21177380 | Human normal bone marrow (NBM) cells were purchased from AllCells Inc. Human ALL samples were obtained from patients at the Stanford University Medical Center, with informed Save | Full Paper |
2011 | Pang, WW;Price, EA;Sahoo, D;Beerman, I;Maloney, WJ;Rossi, DJ;Schrier, SL;Weissman, IL; | Human bone marrow hematopoietic stem cells are increased in frequency and myeloid-biased with age | Proceedings of the National Academy of Sciences of the United States of America | 22123971 | In the human hematopoietic system, aging is associated with decreased bone marrow cellularity, decreased adaptive immune system function, and increased incidence of anemia and Save;Normal young human bone marrow mononuclear cells were purchased fro | Full Paper |
2011 | Jan, M;Chao, MP;Cha, AC;Alizadeh, AA;Gentles, AJ;Weissman, IL;Majeti, R; | Prospective separation of normal and leukemic stem cells based on differential expression of TIM3, a human acute myeloid leukemia stem cell marker | Proceedings of the National Academy of Sciences of the United States of America | 21383193 | Normal human bone marrow mononuclear cells were purchased from AllCells. Human AML samples were obtained from patients at the Stanford Medical Center with informed consent, Save | Full Paper |
2011 | Sahler, J;Bernard, JJ;Spinelli, SL;Blumberg, N;Phipps, RP; | The Feverfew plant-derived compound, parthenolide enhances platelet production and attenuates platelet activation through NF-κB inhibition | Thrombosis research | 21272923 | Adapted from previous procedures [4], human CD34+ cord blood cells were purchased from Allcells (Emeryville, CA) and first plated at 2.5 × 10 5 cells/mL in IMDM supplemented with Save | Full Paper |
2011 | Nahimana, A;Aubry, D;Lagopoulos, L;Greaney, P;Attinger, A;Demotz, S;Dawson, KM;Schapira, M;Tschopp, J;Dupuis, M;Duchosal, MA; | A novel potent Fas agonist for selective depletion of tumor cells in hematopoietic transplants | Blood cancer journal | 22829095 | lture and analysis conditions Human CD34+ cells from cord blood (CB), mobilized peripheral blood (mPB) or bone marrow (BM) were obtained unpurified (mPB) or already purified (CB, mPB, BM) from either AllCells, LLC (San Mateo, CA, USA), or purified us | Full Paper |
2011 | Hart, S;Goh, KC;Novotny-Diermayr, V;Tan, YC;Madan, B;Amalini, C;Ong, LC;Kheng, B;Cheong, A;Zhou, J;Chng, WJ;Wood, JM; | Pacritinib (SB1518), a JAK2/FLT3 inhibitor for the treatment of acute myeloid leukemia | Blood cancer journal | 22829080 | Primary cells, either peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex ( Save | Full Paper |
2011 | Nagao, R;Ashihara, E;Kimura, S;Strovel, JW;Yao, H;Takeuchi, M;Tanaka, R;Hayashi, Y;Hirai, H;Padia, J;Strand, K;Maekawa, T; | Growth inhibition of imatinib-resistant CML cells with the T315I mutation and hypoxia-adaptation by AV65--a novel Wnt/β-catenin signaling inhibitor | Cancer letters | 21906872 | Bone marrow (BM) mononuclear cells obtained from CML patients and healthy volunteers (ALLCells, Emeryville, CA) were plated in duplicate in MethoCult H4434 Classic (StemCell Technologies Inc, Vancouver, Canada) and cultured at 37 °C in 5% CO2. After | Full Paper |
2011 | Gao, N;Budhraja, A;Cheng, S;Liu, EH;Chen, J;Yang, Z;Chen, D;Zhang, Z;Shi, X; | Phenethyl isothiocyanate exhibits antileukemic activity in vitro and in vivo by inactivation of Akt and activation of JNK pathways | Cell death & disease | 21472003 | Fresh normal peripheral blood mononuclear cells were purchased from Allcells (Emeryvill, CA, USA). After washing and enumerating, cells were suspended at 8 × 10 5 /ml before Save | Full Paper |
2011 | Yang, L;Wang, H;Kornblau, SM;Graber, DA;Zhang, N;Matthews, JA;Wang, M;Weber, DM;Thomas, SK;Shah, JJ;Zhang, L;Lu, G;Zhao, M;Muddasani, R;Yoo, SY;Baggerly, KA;Orlowski, RZ; | Evidence of a role for the novel zinc-finger transcription factor ZKSCAN3 in modulating Cyclin D2 expression in multiple myeloma | Oncogene | 21057542 | The current studies on deidentified aliquots were performed according to an Institutional Review Board-approved protocol. Normal human CD19+ B-cell total RNA was from Miltenyi Biotec, Inc., and plasma cell cDNA was from AllCells LLC. (Berkeley, CA) | Full Paper |
2011 | Yu, X;McCarthy, PJ;Lim, HJ;Iempridee, T;Kraus, RJ;Gorlen, DA;Mertz, JE; | The ZIIR element of the Epstein-Barr virus BZLF1 promoter plays a central role in establishment and maintenance of viral latency | Journal of virology | 21389123 | Fresh human primary B cells from peripheral blood of healthy donors were purchased from AllCells (Emeryville, CA). The B cells were infected the day they arrived by incubation at 4C Save | Full Paper |
2011 | Sagawa, M;Shimizu, T;Fukushima, N;Kinoshita, Y;Ohizumi, I;Uno, S;Kikuchi, Y;Ikeda, Y;Yamada-Okabe, H;Kizaki, M; | A new disulfide-linked dimer of a single-chain antibody fragment against human CD47 induces apoptosis in lymphoid malignant cells via the hypoxia inducible factor-1α pathway | Cancer science | 21401803 | CD47 belongs to the immunoglobulin superfamily and is associated with β‐integrins. Recently it was reported that CD47 ligation rapidly induces apoptosis in B‐chronic lymphocytic Save | Full Paper |
2011 | Takeuchi, M;Ashihara, E;Yamazaki, Y;Kimura, S;Nakagawa, Y;Tanaka, R;Yao, H;Nagao, R;Hayashi, Y;Hirai, H;Maekawa, T; | Rakicidin A effectively induces apoptosis in hypoxia adapted Bcr-Abl positive leukemic cells | Cancer science | 21166958 | Bone marrow cells from two normal volunteers and CML patients in the chronic phase before imatinib treatment were purchased from AllCells Inc. (Emeryville, CA, USA). Thawed Save | Full Paper |
2011 | Su, WR;Zhang, QZ;Shi, SH;Nguyen, AL;Le, AD; | Human gingiva-derived mesenchymal stromal cells attenuate contact hypersensitivity via prostaglandin E2-dependent mechanisms | Stem cells (Dayton, Ohio) | 21987520 | CD14+ cells were negatively selected from human peripheral blood mononuclear cells (PBMCs) (AllCells LLC, Emeryville, CA, http://www.allcells.com) using the MACS CD14 MicroBeads (Monocyte Isolation Kit, Miltenyi Biotec, Auburn, CA, http://www.milteny | Full Paper |
2011 | Bissels, U;Wild, S;Tomiuk, S;Hafner, M;Scheel, H;Mihailovic, A;Choi, YH;Tuschl, T;Bosio, A; | Combined characterization of microRNA and mRNA profiles delineates early differentiation pathways of CD133+ and CD34+ hematopoietic stem and progenitor cells | Stem cells (Dayton, Ohio) | 21394831 | tenyi Biotec) using the negative fraction of the first separation. The gating of the separated cells was performed according to ISHAGE. CD133+ cells from mobilized peripheral blood were obtained from AllCells LLC. RNA Sources Total RNA was isolated | Full Paper |
2011 | Mendelson, A;Frank, E;Allred, C;Jones, E;Chen, M;Zhao, W;Mao, JJ; | Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cells in vitro | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 21746864 | Human bone marrow MSCs (AllCells, Emeryville, CA, USA), human ASCs, and human SSCs were isolated from fresh adult donor samples using previously established methods (27-31). MSCs were purified from bone marrow using the RosetteSep Human Mesenchymal S | Full Paper |
2011 | Gao, N;Budhraja, A;Cheng, S;Liu, EH;Huang, C;Chen, J;Yang, Z;Chen, D;Zhang, Z;Shi, X; | Interruption of the MEK/ERK signaling cascade promotes dihydroartemisinin-induced apoptosis in vitro and in vivo | Apoptosis : an international journal on programmed cell death | 21336837 | Fresh normal peripheral blood mononuclear cells were purchased from Allcells (Emeryville, CA, USA). After washing and enumerating as described for mononuclear cells, cells were Save | Full Paper |
2011 | Zhou, L;Opalinska, J;Sohal, D;Yu, Y;Mo, Y;Bhagat, T;Abdel-Wahab, O;Fazzari, M;Figueroa, M;Alencar, C;Zhang, J;Kambhampati, S;Parmar, S;Nischal, S;Hueck, C;Suzuki, M;Freidman, E;Pellagatti, A;Boultwood, J;Steidl, U;Sauthararajah, Y;Yajnik, V;McMahon, C;Gore, SD;Platanias, LC;Levine, R;Melnick, A;Wickrema, A;Greally, JM;Verma, A; | Aberrant epigenetic and genetic marks are seen in myelodysplastic leukocytes and reveal Dock4 as a candidate pathogenic gene on chromosome 7q | The Journal of biological chemistry | 21532034 | 106 CD34+ cells (AllCells) were thawed, cultured for 2 h, resuspended in 100 μl of human CD34+ Nucleofection solution (Amaxa), then transferred into cuvettes, and electroporated using program U008. CD34+ cells were collected and cultured in 24-well p | Full Paper |
2011 | Froelich, S;Tai, A;Kennedy, K;Zubair, A;Wang, P; | Pseudotyping lentiviral vectors with aura virus envelope glycoproteins for DC-SIGN-mediated transduction of dendritic cells | Human gene therapy | 21452926 | Peripheral blood mononuclear cells (PBMCs) from healthy human donors were purchased from AllCells. PBMCs were allowed to adhere for 2 hr on non-tissue culture-treated 24-well plates and washed extensively. Adherent monocytes were plated at a density | Full Paper |
2011 | Jiang, X;Christopherson, GT;Mao, HQ; | The effect of nanofibre surface amine density and conjugate structure on the adhesion and proliferation of human haematopoietic progenitor cells | Interface focus | 23050077 | Frozen human UCB CD133+ cells were purchased from AllCells, LLC (Emeryville, CA, USA). The CD133+ purity in the post-thawed HSPCs was determined by flow cytometry to be 97 per cent and the viability was determined by Trypan blue exclusion assay to be | Full Paper |
2011 | Zhu, J;Shimizu, E;Zhang, X;Partridge, NC;Qin, L; | EGFR signaling suppresses osteoblast differentiation and inhibits expression of master osteoblastic transcription factors Runx2 and Osterix | Journal of cellular biochemistry | 21381079 | The epidermal growth factor receptor (EGFR) and its ligands regulate key processes of cell biology, such as proliferation, survival, differentiation, migration, and tumorigenesis. We Save | Full Paper |
2011 | Khoury, M;Drake, A;Chen, Q;Dong, D;Leskov, I;Fragoso, MF;Li, Y;Iliopoulou, BP;Hwang, W;Lodish, HF;Chen, J; | Mesenchymal stem cells secreting angiopoietin-like-5 support efficient expansion of human hematopoietic stem cells without compromising their repopulating potential | Stem cells and development | 21142526 | Purified human CD133 + cord blood cells were purchased from AllCells. Alternatively, umbilical cord blood was obtained from the National Disease Research Interchange or the Save | Full Paper |
2011 | Hong, L;Zhang, G;Sultana, H;Yu, Y;Wei, Z; | The effects of 17-β estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitro | Stem cells and development | 20735179 | Fresh human bone marrow donated by 12 healthy donors (6 males and 6 females, average age = 27.4 ± 6.1) were purchased (Allcells, Emeryville, CA). Mononuclear cells were isolated using density gradient centrifugation (Histopaque-1.077; Sigma, St. Loui | Full Paper |
2011 | Kunisato, A;Wakatsuki, M;Shinba, H;Ota, T;Ishida, I;Nagao, K; | Direct generation of induced pluripotent stem cells from human nonmobilized blood | Stem cells and development | 20497033 | BM and PB MNCs were obtained from Allcells (ABM007F and PB003F each). Frozen BM MNCs purchased from Allcells were collected from a 25-year-old male donor (lot#BM1705), a Save | Full Paper |
2011 | Basu, J;Genheimer, CW;Sangha, N;Quinlan, SF;Guthrie, KI;Kelley, R;Ilagan, RM;Jain, D;Bertram, T;Ludlow, JW; | Organ specific regenerative markers in peri-organ adipose: kidney | Lipids in health and disease | 21957910 | CD34+ GCSF mobilized/non-mobilized peripheral blood mononuclear cell cDNA was purchased from AllCells LLC. Fetal and adult hepatocyte cDNA and keratinocyte cDNA were Save | Full Paper |
2011 | Gupta, MS;Cooper, ES;Nicoll, SB; | Transforming growth factor-beta 3 stimulates cartilage matrix elaboration by human marrow-derived stromal cells encapsulated in photocrosslinked carboxymethylcellulose hydrogels: potential for nucleus pulposus replacement | Tissue engineering. Part A | 21707438 | hMSCs were isolated from the bone marrow of a 37-year-old man (AllCells, Emeryville, CA) using enriched density centrifugation. Briefly, an hMSC Enrichment Cocktail (StemCell Technologies, Vancouver, BC, Canada)44 was added to the bone marrow and inc | Full Paper |
2011 | McWhirter, J;Noguchi, H;Gompper, G; | Deformation and clustering of red blood cells in microcapillary flows | Soft Matter | Trajectories are shown in a co-moving frame, ie relative to the center-of-mass of allcells. Solid and dashed lines represent the cm of RBCs and their periodic images, respectively. Save | Full Paper | |
2011 | Vanella, L;Kim, DH;Sodhi, K;Barbagallo, I;Burgess, AP;Falck, JR;Schwartzman, ML;Abraham, NG; | Crosstalk between EET and HO-1 downregulates Bach1 and adipogenic marker expression in mesenchymal stem cell derived adipocytes | Prostaglandins & other lipid mediators | 21821145 | Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 10% heat inactivated fetal bovi | Full Paper |
2011 | MacEneaney, OJ;Connick, E;DeSouza, CA; | Effects of HIV-1 gp120 and protease inhibitors on apoptotic susceptibility of CD34+ hematopoietic progenitor cells | Journal of acquired immune deficiency syndromes (1999) | 21233630 | Freshly-isolated CD34+ cells from healthy G-CSF-treated human donors (n=3) were obtained from an independent supplier (Allcells, LLC, Berkeley, CA). Donors tested negative for HIV-1, hepatitis B and hepatitis C. Viability of cells determined by trypa | Full Paper |
2011 | He, JQ;Vu, DM;Hunt, G;Chugh, A;Bhatnagar, A;Bolli, R; | Human cardiac stem cells isolated from atrial appendages stably express c-kit | PloS one | 22140461 | Human bone marrow cells were purchased from AllCells (Emeryville, CA). Total RNA of human carotid artery was bought from Agilent Technologies (Santa Clara, CA). Cells were Save | Full Paper |
2011 | Kobayashi, DT;Olson, RJ;Sly, L;Swanson, CJ;Chung, B;Naryshkin, N;Narasimhan, J;Bhattacharyya, A;Mullenix, M;Chen, KS; | Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analyses | PloS one | 21904622 | For the dilutional linearity experiment, six lots of control PBMCs were purchased from AllCells for further assay testing. After thawing and lysis, PBMCs were serially diluted 4-fold and Save | Full Paper |
2011 | Froelich, S;Tai, A;Kennedy, K;Zubair, A;Wang, P; | Virus-receptor mediated transduction of dendritic cells by lentiviruses enveloped with glycoproteins derived from Semliki Forest virus | PloS one | 21738680 | th 293T.DCSIGN cells for 8 hrs before changing to fresh D10 medium. Transduction of human PBMC-derived DCs Peripheral blood mononuclear cells (PBMC) from two healthy human donors were purchased from AllCells (Emeryville, CA). PBMC were differentiate | Full Paper |
2011 | Danner, R;Chaudhari, SN;Rosenberger, J;Surls, J;Richie, TL;Brumeanu, TD;Casares, S; | Expression of HLA class II molecules in humanized NOD.Rag1KO.IL2RgcKO mice is critical for development and function of human T and B cells | PloS one | 21611197 | ) Tg.NOD.Rag1KO.IL2RγcKO (referred as DRAG) mice were maintained in the pathogen-free WRAIR Veterinary Medicine Facility. Infusion of mice with HSC Umbilical cord bloods (UCB) were purchased from AllCells (Emeryville,CA) and Promocell (Heidelberg, G | Full Paper |
2011 | Drake, AC;Khoury, M;Leskov, I;Iliopoulou, BP;Fragoso, M;Lodish, H;Chen, J; | Human CD34+ CD133+ hematopoietic stem cells cultured with growth factors including Angptl5 efficiently engraft adult NOD-SCID Il2rγ-/- (NSG) mice | PloS one | 21559522 | rch. The MIT committee on animal care (MIT CAC) approved the research as part of animal protocol 0310-027-13. Cell isolation and culture Purified CD133+ human umbilical cord HSCs were purchased from AllCells (California) or National Disease Research | Full Paper |
2011 | Burridge, PW;Thompson, S;Millrod, MA;Weinberg, S;Yuan, X;Peters, A;Mahairaki, V;Koliatsos, VE;Tung, L;Zambidis, ET; | A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability | PloS one | 21494607 | For generation of the non-viral CD34+ cord blood-derived hiPSC lines (CBiPSC6.2, CBiPSC6.11, CBiPSC6.13 and CBiPSC19.11), 0.5-1×106 human CD34+ cord blood cells (AllCells, Emeryville, CA, http://www.allcells.com) were expanded in StemSpan-SFEM | Full Paper |
2011 | Martellini, JA;Cole, AL;Svoboda, P;Stuchlik, O;Chen, LM;Chai, KX;Gangrade, BK;Sørensen, OE;Pohl, J;Cole, AM; | HIV-1 enhancing effect of prostatic acid phosphatase peptides is reduced in human seminal plasma | PloS one | 21283773 | obtained from the National Institutes of Health AIDS Research and Reference Reagent Program (Germantown, MD, USA), and Peripheral Blood Mononuclear Cells (PBMCs) were obtained from healthy donors by AllCells, LLC (Emeryville, CA, USA). TZM-bl cells w | Full Paper |
2011 | Gupta, A;Le, A;Belinka, BA;Kachlany, SC; | In vitro synergism between LFA-1 targeting leukotoxin (Leukothera™) and standard chemotherapeutic agents in leukemia cells | Leukemia research | 21664691 | and maintained in RPMI 1640 medium with 10% fetal bovine serum (Invitrogen, Carlsbad, CA) at 37 °C, 5% CO2. 2.2. Primary cells Frozen primary human cells were purchased from AllCells, LLC. (Emeryville, CA). Viability of these primary cells was 90% | Full Paper |
2011 | Bouchez, LC;Boitano, AE;de Lichtervelde, L;Romeo, R;Cooke, MP;Schultz, PG; | Small-molecule regulators of human stem cell self-renewal | Chembiochem : a European journal of chemical biology | 21381174 | screen: The assay was performed on purified human CD34 + cells from granulocyte colony-stimulating factor-mobilized peripheral blood from normal donors (supplied by AllCells, Save | Full Paper |
2011 | Fukumoto, R;Kiang, JG; | Geldanamycin analog 17-DMAG limits apoptosis in human peripheral blood cells by inhibition of p53 activation and its interaction with heat-shock protein 90 kDa after exposure to ionizing radiation | Radiation research | 21663398 | Exposure to ionizing radiation induces p53, and its inhibition improves mouse survival. We tested the effect of 17-dimethylamino-ethylamino-17-demethoxygeldanamycin (17-DMAG) on Save | Full Paper |
2011 | Pogosova-Agadjanyan, EL;Fan, W;Georges, GE;Schwartz, JL;Kepler, CM;Lee, H;Suchanek, AL;Cronk, MR;Brumbaugh, A;Engel, JH;Yukawa, M;Zhao, LP;Heimfeld, S;Stirewalt, DL; | Identification of radiation-induced expression changes in nonimmortalized human T cells | Radiation research | 21268710 | Peripheral blood samples were collected from healthy volunteer donors at the Fred Hutchinson Cancer Research Center (FHCRC) or purchased from a commercial vendor (AllCells, Emeryville, CA). All samples were obtained under Institutional Review Board ( | Full Paper |
2011 | Bagley, RG;Kurtzberg, L;Rouleau, C;Yao, M;Teicher, BA; | Erufosine, an alkylphosphocholine, with differential toxicity to human cancer cells and bone marrow cells | Cancer chemotherapy and pharmacology | 21526352 | and compounds were added to each well of a 6-well plate in a final volume of 3 mls; RPMI8226 multiple myeloma cells and compounds were added to 3 mls Methocult medium (AllCells Save | Full Paper |
2011 | Hong, L;Krishnamachari, Y;Seabold, D;Joshi, V;Schneider, G;Salem, AK; | Intracellular release of 17-β estradiol from cationic polyamidoamine dendrimer surface-modified poly (lactic-co-glycolic acid) microparticles improves osteogenic differentiation of human mesenchymal stromal cells | Tissue engineering. Part C, Methods | 20883116 | and solvents were purchased from Sigma. All the solvents, including ethyl acetate and dichloromethane (DCM) and acetonitrile, were of high performance liquid chromatography (HPLC) analytical grade. Fresh human bone marrow was purchased from Allcells. | Full Paper |
2011 | McNeish, J;Mathieu, A;Dempsey, B; | Book Review: Bringing stem cell science to the classrooms | Regenerative Medicine | from a small biotechnology company, ReachBio (Seatttle, WA, USA). ReachBio is a contract of clients", so education is clearly a passion at ReachBio. As much of the focus of the text is Save | Full Paper | |
2011 | Nelson, DM;Joseph, B;Hillion, J;Segal, J;Karp, JE;Resar, LM; | Flavopiridol induces BCL-2 expression and represses oncogenic transcription factors in leukemic blasts from adults with refractory acute myeloid leukemia | Leukemia & lymphoma | 21728742 | from the peripheral blood blasts were compared to gene expression in CD34+ hematopoietic stem cells (HSCs); mRNA levels from the HSCs were arbitrarily assigned a value of 1.0. HSC RNA was obtained from a commercial source (ALLCELLS, CA, USA) | Full Paper |
2011 | Ma, K;Fox, L;Shi, G;Shen, J;Liu, Q;Pappas, JD;Cheng, J;Qu, T; | Generation of neural stem cell-like cells from bone marrow-derived human mesenchymal stem cells | Neurological research | 22196762 | The hMSCs from four donors were obtained from AllCells LLC (Emeryville, CA, USA) and Cambrex Bioscience (Walkersville, MD, USA). These hMSCs are negative for surface markers associated with hematopoietic cells (e.g. CD11b, CD33, CD34, and CD133) and | Full Paper |
2011 | Wang, DY;Chang, CW;Lagacé, RE;Oldroyd, NJ;Hennessy, LK; | Development and validation of the AmpFℓSTR® Identifiler® Direct PCR Amplification Kit: a multiplex assay for the direct amplification of single-source samples | Journal of forensic sciences | 21418220 | the performance of the Identifiler Direct Kit on buccal samples deposited on Indicating FTA cards, white blood cells (WBCs) from three individuals were purchased from AllCells, Save | Full Paper |
2011 | Shah, BS;Mao, JJ; | Labeling of mesenchymal stem cells with bioconjugated quantum dots | Methods in molecular biology (Clifton, N.J.) | 21153373 | Bone marrow samples from healthy human donors (AllCells, Berkeley, CA) 2. RosetteSepTM (Stem Cell Technologies, Vancouver, BC, Canada) 3. 35-mm petri dishes (Falcon, Becton Dickinson Labware, Franklin Lakes, NJ) 4. DMEM: Dulbecco’s Modified Eagle’ | Full Paper |
2011 | Li, Z;Chen, J; | In vitro functional study of miR-126 in leukemia | Methods in molecular biology (Clifton, N.J.) | 20931398 | Genomic DNA template isolated from human normal bone marrow mononuclear cells purchased from AllCells (Emeryville, CA). 3. MSCVpuro (Clontech, Mountain View, CA) is a retroviral vector for cloning and gene expression. 4. AML1-ETO fusion gene in a v | Full Paper |
2011 | Kanjevac, T;Milovanovic, M;Volarevic, V;Arsenijevic, N; | Fluoride release from glass ionomer cements correlates with the necrotic death of human dental pulp stem cells | Serbian Journal of Experimental and Clinical Research | Human SHEDs were purchesed from AllCells (Emeryville, California USA). The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS, 100 IU/mL penicillin G and 100 μg/mL streptomycin | Full Paper | |
2011 | Bassan, R;Rohatiner, A;Lerede, T;Carter, M;Di Bona, E;Pogliani, E;Rossi, G;Fabris, P;Morandi, S;Lambertenghi-Deliliers, G;Casula, P;Vespignani, M;Lister, T;Barbui, T; | Early Application of Anthracyclines in Adult Acute Lymphoblastic Leukemia (ALL) | Haematology and Blood Transfusion / Hämatologie und Bluttransfusion | However, because IDR resistance may be dose-dependent, as ascertained by in vitro studies on MDRl+ ALLcells [17], increaseddose IDR may find a place in the treatment of patients Save | Full Paper | |
2011 | Larson, R;Dodge, R;Szatrowski, T;Frankel, S; | Results of Recent Clinical Trials in Acute Lymphoblastic Leukemia by the Cancer and Leukemia Group B | Haematology and Blood Transfusion / Hämatologie und Bluttransfusion | Rapid depletion of asparagine results in selective killing of ALLcells since normal cells are able to synthesize asparagine. Because asparaginase is not markedly myelosuppressive, it is Save | Full Paper | |
2011 | Bissels, U; | Combined analysis of microRNA and mRNA signatures in human hematopoietic stem and progenitor cells using a novel microarray quantification system | Thesis | Granulocyte-colony stimulating factor (G-CSF) mobilized peripheral blood was obtained from healthy donors after informed consent (Allcells, Berkley, USA) and CD133+ cells were Save | Full Paper | |
2011 | Milewski, ME; | The Angiogenic effect of Erythropoietin on Stem Cells In-Vitro | Thesis | HUVEC cells (p-10) were obtained from Allcells, LLC (Emeryville, CA). The cells were removed from the -80°C and placed in a 37°C water bath until thawed. 10 ml of HUVEC media (Allcells, Emeryville, CA) was added to the cells and centrifuged for 5 min | Full Paper | |
2011 | Stewart, HRA; | A novel reporter system where gene knockdown is used to assay intercellular siRNA transfer | Thesis | Mesenchymal stem cells were harvested from bone marrow aspirates purchased from Lonza and AllCells. To isolate human MSCs, nucleated cells were recovered from the 10ml bone marrow aspirates with a density gradient (Ficoll-Paque) and resuspended in co | Full Paper | |
2011 | KC, P; | Proteomic Analysis of Human Mesenchymal Stem Cell Aging during serial sub-culture | Thesis | Human bone marrow was purchased from AllCells, LLC, Emeryvill, CA from a 28 years old donor. Human mesenchymal stem cells were isolated following RosetteSep method and expanded in culture using CFU-F assay. Fluorescence-activated cell sorting (FACS) | Full Paper | |
2011 | Stosich, MS; | Micro-CT evaluation of MSC/HUVEC/HSC-mediated angiogenesis in large PCL-βTCP constructs implanted in nude mice | Thesis | For in vivo study, fresh bone marrow samples of anonymous adult male donors were purchased from AllCells (Berkeley, CA), and used to isolate human mesenchymal stem cells ( Save | Full Paper | |
2011 | Pang, WW;Park, CY;Greenberg, PL; | Separation of normal and aberrant hematopoietic stem cells in myelodysplastic syndrome by CD69 expression | Thesis | Human Samples. Normal young human bone marrow mononuclear cells were purchased from AllCells, Inc. (Emeryville, CA, USA). Normal young and old human bone marrow samples were obtained from hematologically normal donors at the Stanford Medical Center w | Full Paper | |
2011 | Ramos, JH; | Phenotypic and molecular characterization of a human adult bone marrow committed T cell progenitor | Thesis | Human mononuclear cells obtained from fresh, normal adult bone marrow were purchased from AllCells, LLC. (Emeryville, CA). Donors were between the ages of 18 and 55 years, 110 lbs, in good health, and screened for HIV, Hepatitis B and Hepatitis C bef | Full Paper | |
2011 | Thacker, SG; | Type I Interferons and the Development of Impaired Vascular Function and Repair in Human and Murine Lupus | Thesis | lU/ml for 6 hours, followed by RNA harvesting. Similar experiments were performed with a more purified EPC population which was directly obtained from control bone marrow. In brief, CD133+ cells obtained from healthy control bone marrows (AllCells, | Full Paper | |
2011 | Panas, MW; | The identification and elimination of immunosuppressive Mycobacterium bovis BCG genes to create novel rBCG vectors that generate increased CD8+ T cell responses | Thesis | The delivery of an allelic exchange substrate to allcells within a mycobacterial population will be accomplished through specializedtransduction using the temperature sensitive phage Save | Full Paper | |
2011 | Hermann, RJ; | Investigation of Ikaros-dependent transcriptional regulation of novel VPAC1 splice variants during epigenetic transformation | Thesis | In order to identify which PBMC were the cell source for smaller VPAC1 isoforms, purified samples of different cell populations that compose the collective PBMC pool had to be analyzed. cDNA from purified peripheral blood cell populations was purchas | Full Paper | |
2011 | Furman, PA;Murakami, E;Niu, C;Lam, AM;Espiritu, C;Bansal, S;Bao, H;Tolstykh, T;Micolochick Steuer, H;Keilman, M;Zennou, V;Bourne, N;Veselenak, RL;Chang, W;Ross, BS;Du, J;Otto, MJ;Sofia, MJ; | Activity and the metabolic activation pathway of the potent and selective hepatitis C virus pronucleotide inhibitor PSI-353661 | Antiviral research | 21600932 | The human bone marrow stem cell toxicity assay was performed by Reachbio (Seattle, WA). Clonogenic progenitor cells of the erythroid (CFU-E and BFU-E) and myeloid (CFU-GM) Save | Full Paper |
2011 | Lam, AM;Espiritu, C;Murakami, E;Zennou, V;Bansal, S;Micolochick Steuer, HM;Niu, C;Keilman, M;Bao, H;Bourne, N;Veselenak, RL;Reddy, PG;Chang, W;Du, J;Nagarathnam, D;Sofia, MJ;Otto, MJ;Furman, PA; | Inhibition of hepatitis C virus replicon RNA synthesis by PSI-352938, a cyclic phosphate prodrug of β-D-2'-deoxy-2'-α-fluoro-2'-β-C-methylguanosine | Antimicrobial agents and chemotherapy | 21444700 | marrow (Lonza, Walkersville, MD) were performed by Reachbio (Seattle, WA) using a semisolid We also thank Emer Clarke at ReachBio LLC (Seattle, WA) for performing human bone Save | Full Paper |
2011 | Veeranki, S;Duan, X;Panchanathan, R;Liu, H;Choubey, D; | IFI16 protein mediates the anti-inflammatory actions of the type-I interferons through suppression of activation of caspase-1 by inflammasomes | PloS one | 22046441 | y the AIM2 and NLRP3 inflammasomes. Materials and Methods Cells and Treatments Frozen human peripheral blood mononuclear cells (PBMCs), purified CD14+ monocytes and CD19+ B cells were purchased from ReachBio (Seattle, WA). THP-1 cells were purchased | Full Paper |
2011 | Maier, MS; | Setting occupational exposure limits for unstudied pharmaceutical intermediates using an in vitro parallelogram approach | Toxicology mechanisms and methods | 20156007 | The mean ± 1 SD was calculated for triplicate cultures. The laboratory (ReachBio LLC, Seattle, Washington) was blinded to the identify of the test compounds and docetaxel served as the positive control, with blank and vehicle as negative controls | Full Paper |
2011 | Lautenschlager, F; | Cell compliance: cytoskeletal origin and importance for cellular function. | Thesis | Human MSCs (hMSCs) were investigated over the time they needed to double their population 17 times, which corresponds to about eight passages. Cells were obtained from the bone marrow of adult donors (Stem Cell Technologies and ReachBio LLC), isolate | Full Paper | |
2010 | Yelamanchili, SV;Chaudhuri, AD;Chen, LN;Xiong, H;Fox, HS; | MicroRNA-21 dysregulates the expression of MEF2C in neurons in monkey and human SIV/HIV neurological disease | Cell Death Dis | 21170291 | miRNA in situ hybridization. Following necropsy, monkey caudate and hippocampus were formalin fixed, and embedded in paraffin, and 5 μm sections cut onto glass slides. The in situ hybridization was performed at Phylogeny Inc. (Columbus, OH, USA) as d | Full Paper |
2010 | Maret, D;Gruzglin, E;Sadr, MS;Siu, V;Shan, W;Koch, AW;Seidah, NG;Del Maestro, RF;Colman, DR; | Surface expression of precursor N-cadherin promotes tumor cell invasion | Neoplasia | 21170270 | Melanoma, breast, squamous cell, and prostate carcinoma tissue samples and arrays, in addition to corresponding normal tissue, were obtained from FolioBio (Columbus, OH). Metastatic melanoma arrays were from Imgenex (San Diego, CA). Cell Culture and | Full Paper |
2010 | Teichert, A;Elalieh, H;Bikle, D; | Disruption of the hedgehog signaling pathway contributes to the hair follicle cycling deficiency in Vdr knockout mice | J. Cell. Physiol. | 20458748 | The binding of the antibodies was detected by biotinylated goat anti‐rabbit IgG, followed by ABC‐peroxidase reagent (Vector, Burlingame, CA) and revealed with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followed by hematoxyli | Full Paper |
2010 | Kashyap, MK;Harsha, HC;Renuse, S;Pawar, H;Sahasrabuddhe, NA;Kim, MS;Marimuthu, A;Keerthikumar, S;Muthusamy, B;Kandasamy, K;Subbannayya, Y;Prasad, TS;Mahmood, R;Chaerkady, R;Meltzer, SJ;Kumar, RV;Rustgi, AK;Pandey, A; | SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretome | Cancer Biol. Ther. | 20686364 | ... _id":"553574440","term_text":"ESC96101"}}ESC96101 [/protein/ESC96101]) consisted of 34 cases in duplicate, ranging from differentiation grades I–III from patient's ages 36–71 years. The TMAs from FolioBio (catalog # ARY-HH0091) consisted of 40 ES | Full Paper |
2010 | Li, Z;Qi, CF;Shin, DM;Zingone, A;Newbery, HJ;Kovalchuk, AL;Abbott, CM;Morse, HC; | Eef1a2 promotes cell growth, inhibits apoptosis and activates JAK/STAT and AKT signaling in mouse plasmacytomas | PLoS ONE | 20505761 | correction. A tissue microarray of costal biopsies of normal individuals or patients diagnosed with MM was purchased from Folio BioSciences (Columbus, OH). Immunohistochemical studies were detailed previously [27]. Mouse | Full Paper |
2010 | Shih, DM;Xia, YR;Yu, JM;Lusis, AJ; | Temporal and tissue-specific patterns of Pon3 expression in mouse: in situ hybridization analysis | Adv. Exp. Med. Biol. | 20221872 | Detailed biochemical studies are also necessary to identify the physiological substrate(s) of PON3 in various cells/tissues. Notes. Acknowledgment. We thank Yi-Shou Shi, XuPing Wang, and Phylogeny, Inc. (Columbus, OH) for excellent technical support | Full Paper |
2010 | Hill, LS;Richardson, TL;Profeta, LT;Shaw, TJ;Hintz, CJ;Twining, BS;Lawrenz, E;Myrick, ML; | Construction, figures of merit, and testing of a single-cell fluorescence excitation spectroscopy system | Rev Sci Instrum | 20113077 | The relay box uses a Bud Industries PN series box enclosure (model PN-1341-C) as the base. The circuit consisted of two NTE Electronics solid-state relays (model RS3–1D10–51), two Omron Electronic relays (model G4B-112T-C-U.S.-AC120), one Qualtek Ele | Full Paper |
2010 | Gentles, AJ;Plevritis, SK;Majeti, R;Alizadeh, AA; | Association of a leukemic stem cell gene expression signature with clinical outcomes in acute myeloid leukemia | JAMA | 21177505 | Normal human bone marrow mononuclear cells were purchased from AllCells Inc (Emeryville, California), and human cord blood was obtained from Stanford University Medical Center. Save | Full Paper |
2010 | Carter, CC;Onafuwa-Nuga, A;McNamara, LA;Riddell, J;Bixby, D;Savona, MR;Collins, KL; | HIV-1 infects multipotent progenitor cells causing cell death and establishing latent cellular reservoirs | Nature medicine | 20208541 | minates all consensus AP-1, NF-AT, NF-κB SP-1 and TATA motifs, but maintains the polyA site found in the R region. Cell culture Fresh whole bone marrow aspirates were obtained commercially (AllCells ltd.). Pre-existing umbilical cord blood lacking p | Full Paper |
2010 | Naka, K;Hoshii, T;Muraguchi, T;Tadokoro, Y;Ooshio, T;Kondo, Y;Nakao, S;Motoyama, N;Hirao, A; | TGF-beta-FOXO signalling maintains leukaemia-initiating cells in chronic myeloid leukaemia | Nature | 20130650 | solid medium. Human LICs were purified as CD34+CD38-Lin- cells of bone marrow cells from CML patients (Cureline, Inc. and AllCells). These cells were co-cultured on OP-9 cells for 5 days before transfer to semi-solid medium. Cell cycling was assessed | Full Paper |
2010 | Lishko, PV;Botchkina, IL;Fedorenko, A;Kirichok, Y; | Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channel | Cell | 20144758 | Frozen normal peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryville, CA), thawed and plated onto sterile coverslips according to the manufacturer instructions. Mouse monoclonal anti-COXIV and anti-CD14 antibody were from | Full Paper |
2010 | Loewer, S;Cabili, MN;Guttman, M;Loh, YH;Thomas, K;Park, IH;Garber, M;Curran, M;Onder, T;Agarwal, S;Manos, PD;Datta, S;Lander, ES;Schlaeger, TM;Daley, GQ;Rinn, JL; | Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells | Nature genetics | 21057500 | The CD34 + cells used were mobilized peripheral blood CD34+ cells (AllCells, mBP014F). Cells were thawed and cultured as described 21 for two days before RNA isolation. Save | Full Paper |
2010 | Pichiorri, F;Suh, SS;Rocci, A;De Luca, L;Taccioli, C;Santhanam, R;Zhou, W;Benson, DM;Hofmainster, C;Alder, H;Garofalo, M;Di Leva, G;Volinia, S;Lin, HJ;Perrotti, D;Kuehl, M;Aqeilan, RI;Palumbo, A;Croce, CM; | Downregulation of p53-inducible microRNAs 192, 194, and 215 impairs the p53/MDM2 autoregulatory loop in multiple myeloma development | Cancer cell | 20951946 | IRB-approved procurement protocol (2000C0247) at The Ohio State University and The University of Turin GIMEMA-MM-03-05, N. EUDRACT 2005-004745-33). Three of the five healthy PCs used were purchased from AllCells LLC. (Emeryville, CA) | Full Paper |
2010 | Figueroa, ME;Lugthart, S;Li, Y;Erpelinck-Verschueren, C;Deng, X;Christos, PJ;Schifano, E;Booth, J;van Putten, W;Skrabanek, L;Campagne, F;Mazumdar, M;Greally, JM;Valk, PJ;Löwenberg, B;Delwel, R;Melnick, A; | DNA methylation signatures identify biologically distinct subtypes in acute myeloid leukemia | Cancer cell | 20060365 | We hypothesized that DNA methylation distributes into specific patterns in cancer cells, which reflect critical biological differences. We therefore examined the methylation profiles of 344 Save | Full Paper |
2010 | Liu, W;Lee, HW;Liu, Y;Wang, R;Rodgers, GP; | Olfactomedin 4 is a novel target gene of retinoic acids and 5-aza-2'-deoxycytidine involved in human myeloid leukemia cell growth, differentiation, and apoptosis | Blood | 20724538 | Peripheral blood mononuclear cells from AML patients and normal individuals, and neutrophils from normal individuals, were obtained commercially from AllCells. The neutrophils were Save | Full Paper |
2010 | Tatsumi, K;Taatjes, D;Bouchard, B;Trotman, W;Bovill, E; | Cell Adhesion Molecule 1 (CADM1), a Novel Venous Thrombosis Risk Factor, Is Ubiquitously Present In Vascular Endothelium and Smooth Muscle Cells | Blood | Immunoelectron microscopy (IEM) was performed on cultured human umbilical vein endothelial cells (Allcells LLC, Emeryville, CA) using a post embedding procedure with protein-A Save | Full Paper | |
2010 | Lin, X;Rice, K;Carroll, M;Costa, F;Kilpivaara, O;Soares, M;Levine, R;Licht, J; | Mir-9 Is Aberrantly Expressed In MPN Patients and Accelerates Erythropoietic Cell Growth and Differentiation | Blood | To determine how deregulated expression of miR-9 affected erythropoiesis, we transduced human bone marrow CD34+ cells (AllCells) with empty vector or miR-9 lentivirus, and Save;Abstract 1983 The myeloproliferative neoplasms (MPNs), P | Full Paper | |
2010 | Campbell, JE;Brummel-Ziedins, KE;Butenas, S;Mann, KG; | Cellular regulation of blood coagulation: a model for venous stasis | Blood | 20864579 | We have adapted the corn-trypsin inhibitor whole-blood model to include EA.hy926 as an endothelium surrogate to evaluate the vascular modulation of blood coagulation initiated by Save | Full Paper |
2010 | Sangokoya, C;Telen, MJ;Chi, JT; | microRNA miR-144 modulates oxidative stress tolerance and associates with anemia severity in sickle cell disease | Blood | 20709907 | Normal hemoglobin (HbAA) CD34+ cells (ALLCells) and HbSS CD34+ cells were purified from blood collected from apheresis with donor consent according to Duke Institutional Review Board protocol. Mononuclear cells were separated by Ficoll-Hypaque densit | Full Paper |
2010 | Prabhala, RH;Pelluru, D;Fulciniti, M;Prabhala, HK;Nanjappa, P;Song, W;Pai, C;Amin, S;Tai, YT;Richardson, PG;Ghobrial, IM;Treon, SP;Daley, JF;Anderson, KC;Kutok, JL;Munshi, NC; | Elevated IL-17 produced by TH17 cells promotes myeloma cell growth and inhibits immune function in multiple myeloma | Blood | 20395418 | approval by the institutional review board (IRB) at Dana-Farber Cancer Institute. Healthy donor samples were obtained from the blood donor center at Children's Hospital, Boston, MA. Normal donor bone marrow samples were obtained from AllCells | Full Paper |
2010 | Liu, X;Shin, N;Koblish, HK;Yang, G;Wang, Q;Wang, K;Leffet, L;Hansbury, MJ;Thomas, B;Rupar, M;Waeltz, P;Bowman, KJ;Polam, P;Sparks, RB;Yue, EW;Li, Y;Wynn, R;Fridman, JS;Burn, TC;Combs, AP;Newton, RC;Scherle, PA; | Selective inhibition of IDO1 effectively regulates mediators of antitumor immunity | Blood | 20197554 | Bone marrow mononuclear cells of patients with AML were purchased from ALLCELLS and maintained according to the recommended culture conditions. Save | Full Paper |
2010 | Quintás-Cardama, A;Vaddi, K;Liu, P;Manshouri, T;Li, J;Scherle, PA;Caulder, E;Wen, X;Li, Y;Waeltz, P;Rupar, M;Burn, T;Lo, Y;Kelley, J;Covington, M;Shepard, S;Rodgers, JD;Haley, P;Kantarjian, H;Fridman, JS;Verstovsek, S; | Preclinical characterization of the selective JAK1/2 inhibitor INCB018424: therapeutic implications for the treatment of myeloproliferative neoplasms | Blood | 20130243 | Mononuclear cells were isolated from peripheral blood from patients with PV or normal control persons by centrifugation through Ficoll (Allcells). A total of 2 × 10 5 cells from control or Save | Full Paper |
2010 | Swift, S;Ellison, AR;Kassner, P;McCaffery, I;Rossi, J;Sinclair, AM;Begley, CG;Elliott, S; | Absence of functional EpoR expression in human tumor cell lines | Blood | 20124514 | Erythroid progenitor cells were generated from CD34 + peripheral blood cells mobilized with granulocyte colony-stimulating factor (G-CSF; ALLCELLS) cultured for 6 days in StemSpan Save | Full Paper |
2010 | Driscoll, JJ;Pelluru, D;Lefkimmiatis, K;Fulciniti, M;Prabhala, RH;Greipp, PR;Barlogie, B;Tai, YT;Anderson, KC;Shaughnessy, JD;Annunziata, CM;Munshi, NC; | The sumoylation pathway is dysregulated in multiple myeloma and is associated with adverse patient outcome | Blood | 19965618 | Fresh, normal, human adult bone marrow was from AllCells, and CD138 + cells were affinity purified by positive selection using Miltenyi CD138 + microbeads (Miltenyi Biotec) according Save | Full Paper |
2010 | Lee, CH;Shah, B;Moioli, EK;Mao, JJ; | CTGF directs fibroblast differentiation from human mesenchymal stem/stromal cells and defines connective tissue healing in a rodent injury model | The Journal of clinical investigation | 20679726 | Human MSCs were isolated from fresh whole bone marrow samples of 2 anonymous adult donors (age range, 20-25 years; AllCells) and were exempt from IRB approval. Nonhematopoietic, mononucleated and adherent cells were purified by centrifugation through | Full Paper |
2010 | Chao, MP;Jaiswal, S;Weissman-Tsukamoto, R;Alizadeh, AA;Gentles, AJ;Volkmer, J;Weiskopf, K;Willingham, SB;Raveh, T;Park, CY;Majeti, R;Weissman, IL; | Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47 | Science translational medicine | 21178137 | Normal human bone marrow mononuclear cells were purchased from AllCells Inc. Normal peripheral blood and human cancer samples were obtained from patients at the Stanford Save | Full Paper |
2010 | Collins, JM;Ayala, P;Desai, TA;Russell, B; | Three-dimensional culture with stiff microstructures increases proliferation and slows osteogenic differentiation of human mesenchymal stem cells | Small (Weinheim an der Bergstrasse, Germany) | 19943257 | however, experiments were performed on P2 hMSCs obtained from 2 separate donors. Additionally, some experiments were repeated with P2 hMSCs isolated from whole marrow (AllCells, Berkely, CA) as described previously.[29] | Full Paper |
2010 | Cheng, S;Gao, N;Zhang, Z;Chen, G;Budhraja, A;Ke, Z;Son, YO;Wang, X;Luo, J;Shi, X; | Quercetin induces tumor-selective apoptosis through downregulation of Mcl-1 and activation of Bax | Clinical cancer research : an official journal of the American Association for Cancer Research | 21138867 | Mononuclear cells were isolated from peripheral blood or bone marrow of leukemia patients or healthy donors were purchased from AllCells, LLC. Mononuclear cells were suspended Save | Full Paper |
2010 | Berkofsky-Fessler, W;Buzzai, M;Kim, MK;Fruchtman, S;Najfeld, V;Min, DJ;Costa, FF;Bischof, JM;Soares, MB;McConnell, MJ;Zhang, W;Levine, R;Gilliland, DG;Calogero, R;Licht, JD; | Transcriptional profiling of polycythemia vera identifies gene expression patterns both dependent and independent from the action of JAK2V617F | Clinical cancer research : an official journal of the American Association for Cancer Research | 20601445 | Normal bone marrow CD34 + cells were purchased from Allcells. To isolate CD34 + cells, bone marrow samples were diluted 1:1 in PBS and layered onto Histopaque 1077 (Sigma; Save | Full Paper |
2010 | Marchi, E;Paoluzzi, L;Scotto, L;Seshan, VE;Zain, JM;Zinzani, PL;O'Connor, OA; | Pralatrexate is synergistic with the proteasome inhibitor bortezomib in in vitro and in vivo models of T-cell lymphoid malignancies | Clinical cancer research : an official journal of the American Association for Cancer Research | 20501616 | Peripheral blood mononuclear cells (PBMC) from healthy donors were purchased from Allcells. All cell lines were grown as previously described (21). Save | Full Paper |
2010 | Paoluzzi, L;Scotto, L;Marchi, E;Zain, J;Seshan, VE;O'Connor, OA; | Romidepsin and belinostat synergize the antineoplastic effect of bortezomib in mantle cell lymphoma | Clinical cancer research : an official journal of the American Association for Cancer Research | 20068080 | Mononuclear cells from PBMC samples of healthy donors were purchased from Allcells. All cell lines were grown as described previously (12, 13). Save | Full Paper |
2010 | Brunckhorst, MK;Wang, H;Lu, R;Yu, Q; | Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis | Cancer research | 20823154 | Human umbilical vein ECs (HUVEC) and normal human astrocytes (NHA) were from AllCells, Inc., U87MG was obtained from American Type Culture Collection, and U251 was Save | Full Paper |
2010 | Cho, SY;Xu, M;Roboz, J;Lu, M;Mascarenhas, J;Hoffman, R; | The effect of CXCL12 processing on CD34+ cell migration in myeloproliferative neoplasms | Cancer research | 20388788 | Fresh normal marrow was purchased from AllCells LLC. Cord blood (CB) collections were provided by Cord Blood Bank of New York Blood Center. Save | Full Paper |
2010 | Xu, Y;Stamenkovic, I;Yu, Q; | CD44 attenuates activation of the hippo signaling pathway and is a prime therapeutic target for glioblastoma | Cancer research | 20197461 | C, expression of endogenous CD44 in a panel of human glioma cells and NHAs (ALLCELLS, Inc.) was determined by Western blotting using anti-CD44 antibody (Santa Cruz). Total Save | Full Paper |
2010 | Hart, S;Goh, K;Tan, Y;Amalini, C;Wood, J; | Abstract 2524: SB1518, a novel oral JAK2-FLT3 inhibitor, is efficacious in models of acute myeloid leukemia and primary leukemic cells from patients | Cancer Research | For progenitor expansion, PBMCs of AML patients (AllCells Inc) were cultured using a medium containing IL-3, IL-6, SCF and FLT3L (StemCell Technologies TB 28440). The expanded Save | Full Paper | |
2010 | Santanam, U;Zanesi, N;Efanov, A;Costinean, S;Palamarchuk, A;Hagan, JP;Volinia, S;Alder, H;Rassenti, L;Kipps, T;Croce, CM;Pekarsky, Y; | Chronic lymphocytic leukemia modeled in mouse by targeted miR-29 expression | Proceedings of the National Academy of Sciences of the United States of America | 20566844 | Real-time PCR experiments were carried out using miR-29a, miR-29b, and PXDN assays for real-time PCR (Applied Biosystems) according to the manufacturer's protocol. Control human cord blood CD19+ B cells were purchased from Allcells and Lonza | Full Paper |
2010 | Mi, S;Li, Z;Chen, P;He, C;Cao, D;Elkahloun, A;Lu, J;Pelloso, LA;Wunderlich, M;Huang, H;Luo, RT;Sun, M;He, M;Neilly, MB;Zeleznik-Le, NJ;Thirman, MJ;Mulloy, JC;Liu, PP;Rowley, JD;Chen, J; | Aberrant overexpression and function of the miR-17-92 cluster in MLL-rearranged acute leukemia | Proceedings of the National Academy of Sciences of the United States of America | 20133587 | obtained at the time of diagnosis or relapse and with informed consent at the University of Chicago or other hospitals (see characteristics of patients in Table S2). Normal control cells were purchased from AllCells. (Emeryville, CA) | Full Paper |
2010 | Wu, PK;Ringeisen, BR; | Development of human umbilical vein endothelial cell (HUVEC) and human umbilical vein smooth muscle cell (HUVSMC) branch/stem structures on hydrogel layers via biological laser printing (BioLP) | Biofabrication | 20811126 | HUVEC were purchased from Allcells, and HUVSMC were purchased from ATCC. Matrigel was purchased from BD Bioscience. The cells were grown in a humidified environment with 5 Save | Full Paper |
2010 | Bhatwadekar, AD;Guerin, EP;Jarajapu, YP;Caballero, S;Sheridan, C;Kent, D;Kennedy, L;Lansang, MC;Ruscetti, FW;Pepine, CJ;Higgins, PJ;Bartelmez, SH;Grant, MB; | Transient inhibition of transforming growth factor-beta1 in human diabetic CD34+ cells enhances vascular reparative functions | Diabetes | 20460428 | CD34 + cells enriched by Miltenyi positive selection from human cord blood and bone marrow obtained from AllCells (Emeryville, CA) and were >95% pure as assessed by fluorescence Save | Full Paper |
2010 | Lin, TH;Hegen, M;Quadros, E;Nickerson-Nutter, CL;Appell, KC;Cole, AG;Shao, Y;Tam, S;Ohlmeyer, M;Wang, B;Goodwin, DG;Kimble, EF;Quintero, J;Gao, M;Symanowicz, P;Wrocklage, C;Lussier, J;Schelling, SH;Hewet, AG;Xuan, D;Krykbaev, R;Togias, J;Xu, X;Harrison, R;Mansour, T;Collins, M;Clark, JD;Webb, ML;Seidl, KJ; | Selective functional inhibition of JAK-3 is sufficient for efficacy in collagen-induced arthritis in mice | Arthritis and rheumatism | 20506481 | Cryopreserved peripheral blood mononuclear cells (PBMCs; AllCells) were cultured in complete RPMI 1640 medium (supplemented with 10% fetal bovine serum, penicillin/streptomycin, 2 mM L-glutamine, and 1 mM sodium pyruvate) plus 10 μg/ml lectin phytohe | Full Paper |
2010 | Kodippili, GC;Spector, J;Kang, GE;Liu, H;Wickrema, A;Ritchie, K;Low, PS; | Analysis of the kinetics of band 3 diffusion in human erythroblasts during assembly of the erythrocyte membrane skeleton | British journal of haematology | 20553270 | Baxter Healthcare, Inc., Deerfield, IL) from commercially available growth factor mobilized blood samples (AllCells LLC, Emeryville, CA), as described elsewhere (Kang, et al 2008). Briefly, CD34+ cells isolated from growth-factor mobilized peripheral | Full Paper |
2010 | Lavelle, D;Saunthararajah, Y;Vaitkus, K;Singh, M;Banzon, V;Phiasivongsva, P;Redkar, S;Kanekal, S;Bearss, D;Shi, C;Inloes, R;DeSimone, J; | S110, a novel decitabine dinucleotide, increases fetal hemoglobin levels in baboons (P. anubis) | Journal of translational medicine | 20932295 | Frozen CD34+ human cells purified from the peripheral blood of mobilized donors were purchased from Allcells, Inc. These cells were cultured in Iscove's media containing 20% fetal Save | Full Paper |
2010 | Yamaza, T;Kentaro, A;Chen, C;Liu, Y;Shi, Y;Gronthos, S;Wang, S;Shi, S; | Immunomodulatory properties of stem cells from human exfoliated deciduous teeth | Stem cell research & therapy | 20504286 | Healthy bone marrow aspirates from iliac bone and peripheral blood mononuclear cells (PBMNCs) of healthy volunteers were purchased from AllCells (Berkeley, CA, USA). Save | Full Paper |
2010 | Luo, XM;Lei, MY;Feidi, RA;West, AP;Balazs, AB;Bjorkman, PJ;Yang, L;Baltimore, D; | Dimeric 2G12 as a potent protection against HIV-1 | PLoS pathogens | 21187894 | ting amounts of monomer or dimer from the separated purified species. Humanized mice, passive transfer, and HIV-1 challenge Frozen human cord blood CD34+ cells from single donors were purchased from AllCells (Emeryville, CA) or Lonza (Basel, Switzer | Full Paper |
2010 | Ellis-Connell, AL;Iempridee, T;Xu, I;Mertz, JE; | Cellular microRNAs 200b and 429 regulate the Epstein-Barr virus switch between latency and lytic replication | Journal of virology | 20668090 | Raji cell assay as described above. Approximately 1 × 105 GRUs/ml was used to infect 1 × 106 cells/ml of primary blood B cells (AllCells, Emeryville, CA). Samples were harvested at the times indicated in Fig. 2 and processed as described above for ZE | Full Paper |
2010 | Li, J;Favata, M;Kelley, JA;Caulder, E;Thomas, B;Wen, X;Sparks, RB;Arvanitis, A;Rogers, JD;Combs, AP;Vaddi, K;Solomon, KA;Scherle, PA;Newton, R;Fridman, JS; | INCB16562, a JAK1/2 selective inhibitor, is efficacious against multiple myeloma cells and reverses the protective effects of cytokine and stromal cell support | Neoplasia (New York, N.Y.) | 20072651 | Primary bone marrow CD138+ plasma cells from a newly diagnosed MM patient were purchased from Allcells (Emeryville, CA). The cells were cultured in the same medium used for Save | Full Paper |
2010 | Jekle, A;Chhabra, M;Lochner, A;Meier, S;Chow, E;Brandt, M;Sankuratri, S;Cammack, N;Heilek, G; | Epitope switching as a novel escape mechanism of HIV to CCR5 monoclonal antibodies | Antimicrobial agents and chemotherapy | 19995923 | 10% FBS and 1% penicillin-streptomycin. Human peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA), stimulated for 1 day in PBMC medium (RPMI 1640 medium containing 10% FBS, 1% penicillin-streptomycin, 2 mM l-glutami | Full Paper |
2010 | Culpin, RE;Proctor, SJ;Angus, B;Crosier, S;Anderson, JJ;Mainou-Fowler, T; | A 9 series microRNA signature differentiates between germinal centre and activated B-cell-like diffuse large B-cell lymphoma cell lines | International journal of oncology | 20596664 | streptomycin (Gibco), according to the repositories guidelines. Cultures were incubated at 37˚C in a 5% CO2 humidified atmosphere. CD19+ negatively-selected B-cell total RNA (pooled from 3 unrelated donors) served as the normal healthy B-cell control | Full Paper |
2010 | Collins, JM;Goldspink, PH;Russell, B; | Migration and proliferation of human mesenchymal stem cells is stimulated by different regions of the mechano-growth factor prohormone | Journal of molecular and cellular cardiology | 20875825 | 2 mM L-glutamine, 100 units/mL penicillin and 100 μg/mL streptomycin. hMSCs were obtained from the Tulane University Center for Gene Therapy or AllCells (Berkeley, CA). Passage 2-3 (P2-P3) hMSCs were used for all experiments | Full Paper |
2010 | Destache, CJ;Belgum, T;Goede, M;Shibata, A;Belshan, MA; | Antiretroviral release from poly(DL-lactide-co-glycolide) nanoparticles in mice | The Journal of antimicrobial chemotherapy | 20729545 | Frozen elutriated monocytes were obtained from AllCells, LLC (Emeryville, CA, USA). Cells were thawed in a water bath at 37C and counted using Trypan Blue exclusion. Cells (5 × 10 Save | Full Paper |
2010 | Berahovich, RD;Zabel, BA;Penfold, ME;Lewén, S;Wang, Y;Miao, Z;Gan, L;Pereda, J;Dias, J;Slukvin, II;McGrath, KE;Jaen, JC;Schall, TJ; | CXCR7 protein is not expressed on human or mouse leukocytes | Journal of immunology (Baltimore, Md. : 1950) | 20889540 | Human normal bone marrow cells were purchased from AllCells (Emeryville, CA). Human thymocytes were obtained by mechanical disruption of fetal thymus tissue purchased from Save | Full Paper |
2010 | Thacker, SG;Berthier, CC;Mattinzoli, D;Rastaldi, MP;Kretzler, M;Kaplan, MJ; | The detrimental effects of IFN-α on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefaction | Journal of immunology (Baltimore, Md. : 1950) | 20805419 | Similar experiments were performed with EPCs directly obtained from control bone marrow. In brief, CD133+ cells obtained from healthy control bone marrows (AllCells, Emeryville, CA) were thawed following manufacturer’s recommendations and then cultur | Full Paper |
2010 | Toti, US;Guru, BR;Grill, AE;Panyam, J; | Interfacial activity assisted surface functionalization: a novel approach to incorporate maleimide functional groups and cRGD peptide on polymeric nanoparticles for targeted drug delivery | Molecular pharmaceutics | 20527782 | methyl tri-chlorosilane were from Sigma-Aldrich (St Louis, MO). RPMI cell media, fetal bovine serum, and trypsin were purchased from Invitrogen (Carlsbad, CA). HUVEC media was supplied by AllCells (Emeryville, CA) | Full Paper |
2010 | Medina, RJ;O'Neill, CL;Humphreys, MW;Gardiner, TA;Stitt, AW; | Outgrowth endothelial cells: characterization and their potential for reversing ischemic retinopathy | Investigative ophthalmology & visual science | 20554606 | of 1 × 107 cells/mL. Population doubling was carefully monitored, and OECs at early passages were used for all experiments. Frozen human cord blood mononuclear cells were purchased (Allcells; Stem Cell Technologies, Emeryville, CA) and plated under t | Full Paper |
2010 | Kopher, RA;Penchev, VR;Islam, MS;Hill, KL;Khosla, S;Kaufman, DS; | Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cells | Bone | 20601304 | Mayo Institutional Review Board and obtaining informed consent, or isolated from whole bone marrow (AllCells, Emeryville, CA, USA) using standard methods [50]. BM-MSCs were cultured in MSC media as described above. Adherent cells were selected for du | Full Paper |
2010 | Vanella, L;Kim, DH;Asprinio, D;Peterson, SJ;Barbagallo, I;Vanella, A;Goldstein, D;Ikehara, S;Kappas, A;Abraham, NG; | HO-1 expression increases mesenchymal stem cell-derived osteoblasts but decreases adipocyte lineage | Bone | 19853072 | Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential Save | Full Paper |
2010 | Kim, DH;Vanella, L;Inoue, K;Burgess, A;Gotlinger, K;Manthati, VL;Koduru, SR;Zeldin, DC;Falck, JR;Schwartzman, ML;Abraham, NG; | Epoxyeicosatrienoic acid agonist regulates human mesenchymal stem cell-derived adipocytes through activation of HO-1-pAKT signaling and a decrease in PPARγ | Stem cells and development | 20412023 | Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α Save | Full Paper |
2010 | Fox, LE;Shen, J;Ma, K;Liu, Q;Shi, G;Pappas, GD;Qu, T;Cheng, J; | Membrane properties of neuron-like cells generated from adult human bone-marrow-derived mesenchymal stem cells | Stem cells and development | 20394468 | The hMeSCs from 4 donors were commercially obtained from AllCells LLC and Cambrex Bioscience. The cell lines were negative for surface markers associated with hematopoietic Save | Full Paper |
2010 | Lai, Y;Sun, Y;Skinner, CM;Son, EL;Lu, Z;Tuan, RS;Jilka, RL;Ling, J;Chen, XD; | Reconstitution of marrow-derived extracellular matrix ex vivo: a robust culture system for expanding large-scale highly functional human mesenchymal stem cells | Stem cells and development | 19737070 | Freshly isolated human bone marrow mononuclear cells obtained from 20- to 30-year-old donors were purchased from ALLCELLS (Emeryville, CA), and grown on tissue culture plastic Save | Full Paper |
2010 | McLaughlin, J;Markovtsov, V;Li, H;Wong, S;Gelman, M;Zhu, Y;Franci, C;Lang, D;Pali, E;Lasaga, J;Low, C;Zhao, F;Chang, B;Gururaja, TL;Xu, W;Baluom, M;Sweeny, D;Carroll, D;Sran, A;Thota, S;Parmer, M;Romane, A;Clemens, G;Grossbard, E;Qu, K;Jenkins, Y;Kinoshita, T;Taylor, V;Holland, SJ;Argade, A;Singh, R;Pine, P;Payan, DG;Hitoshi, Y; | Preclinical characterization of Aurora kinase inhibitor R763/AS703569 identified through an image-based phenotypic screen | Journal of cancer research and clinical oncology | 19609559 | Cultured human primary mast cells were established from human umbilical cord blood CD34 + progenitor cells (AllCells, LLC, Berkeley, CA) using the method described in US patent no Save | Full Paper |
2010 | Chen, NK;Tan, SY;Udolph, G;Kon, OL; | Insulin expressed from endogenously active glucose-responsive EGR1 promoter in bone marrow mesenchymal stromal cells as diabetes therapy | Gene therapy | 20182520 | HBMMSC (AllCells, Emeryville, CA, USA) cultured overnight in Dulbecco's modified Eagle's medium (DMEM)-3 mM glucose (as the low glucose baseline) were exposed to DMEM-20 mM glucose (high glucose) for 15 or 30 min, and then returned to DMEM-3 mM gluco | Full Paper |
2010 | Marchi, E; | Pralatrexate sinergizza in vitro ed in vivo con l'inibitore del Proteosoma Bortezomib in modelli pre-clinici di Linfoma Non Hodkin T | Thesis | aggiunto a giorni alterni. Le cellule sono state mantenute in coltura alla concentrazione ideale di 0.5 x 106 cellule/mL e trattate come descritto in precedenti studi pubblicati52 . Le cellule mononucleate del sangue periferico da donatori volontari | Full Paper | |
2010 | Qureshi, A;Subathra, M;Grey, A;Schey, K;Del Poeta, M;Luberto, C; | Role of sphingomyelin synthase in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformans | PloS one | 21203393 | Approximately 6.4×10 4 fresh human normal peripheral blood-neutrophils per ml (AllCells) were incubated in 124 µl sterile filtered PBS supplemented with 10% pooled human serum Save | Full Paper |
2010 | O'Connell, RM;Balazs, AB;Rao, DS;Kivork, C;Yang, L;Baltimore, D; | Lentiviral vector delivery of human interleukin-7 (hIL-7) to human immune system (HIS) mice expands T lymphocyte populations | PloS one | 20700454 | 2×10 7 PBMCs (AllCells) that were first labeled with CFSE. HIS mice were created as described[5]. In brief, 1.5×10 5 highly purified CD34+ CB stem cells (AllCells) were injected into the Save | Full Paper |
2010 | Smith, B;Treadwell, J;Zhang, D;Ly, D;McKinnell, I;Walker, PR;Sikorska, M; | Large-scale expression analysis reveals distinct microRNA profiles at different stages of human neurodevelopment | PloS one | 20559549 | obtained from ScienCell Research Laboratories (Carlsbad, CA) and were directly pelleted for RNA extraction. Frozen primary human fetal astrocytes (PHAf) from 20-21 week fetuses were received from ALLCELLS (Emeryville, CA) and were cultured in Astroc | Full Paper |
2010 | Kachlany, SC;Schwartz, AB;Balashova, NV;Hioe, CE;Tuen, M;Le, A;Kaur, M;Mei, Y;Rao, J; | Anti-leukemia activity of a bacterial toxin with natural specificity for LFA-1 on white blood cells | Leukemia research | 19747730 | The oral bacterium, Aggregatibacter actinomycetemcomitans, produces a leukotoxin (LtxA) that is specific for white blood cells (WBCs) from humans and Old World primates by Save | Full Paper |
2010 | Medina, RJ;O'Neill, CL;Sweeney, M;Guduric-Fuchs, J;Gardiner, TA;Simpson, DA;Stitt, AW; | Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identities | BMC medical genomics | 20465783 | l approval from three female volunteer subjects aged 25-35, non-smokers, not receiving any medication and without any clinical diagnosis; while CB-derived mononuclear cells (MNCs) were purchased from AllCells LLC (California, USA). MNCs were isolated | Full Paper |
2010 | Rowe, DD;Leonardo, CC;Hall, AA;Shahaduzzaman, MD;Collier, LA;Willing, AE;Pennypacker, KR; | Cord blood administration induces oligodendrocyte survival through alterations in gene expression | Brain research | 20883670 | Oligodendrocytes (OLs), the predominant cell type found in cerebral white matter, are essential for structural integrity and proper neural signaling. Very little is known concerning stroke- Save | Full Paper |
2010 | Jin, Q;Alkhatib, B;Cornetta, K;Alkhatib, G; | Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1 | Virology | 19913864 | streptomycin sulfate (0.1 mg/ml) in a humidified incubator held at 5% CO2. All cell culture media were purchased from GIBCO. Normal Human Astrocytes (NHA) and their basal medium (with 10% FBS) were purchased from AllCells (AllCells, LLC., Emeryville, | Full Paper |
2010 | Walczak-Drzewiecka, A;Ratajewski, M;Pułaski, Ł;Dastych, J; | DNA methylation-dependent suppression of HIF1A in an immature hematopoietic cell line HMC-1 | Biochemical and biophysical research communications | 19995553 | cDNAs from BM CD34+, PB CD34+, BM MNCs, PB MNCs and BM MSCs purchased from AllCells (Emeryville, CA, USA) was obtained from a population of positive cells (purity exceeding 95%) collected from five individual donors. The level of cognate cDNA was mea | Full Paper |
2010 | Li, W;Hong, L;Hu, L;Magin, RL; | Magnetization transfer imaging provides a quantitative measure of chondrogenic differentiation and tissue development | Tissue engineering. Part C, Methods | 20373975 | Mesenchymal stem cells (MSCs), isolated from fresh adult human bone marrow obtained from AllCells LLC (Berkeley, CA), were plated at a density of 107 nucleated cells per 100-mm-diameter Petri dish. The cells were incubated in a basic medium of Dulbec | Full Paper |
2010 | Akers, SM;O'Leary, HA;Minnear, FL;Craig, MD;Vos, JA;Coad, JE;Gibson, LF; | VE-cadherin and PECAM-1 enhance ALL migration across brain microvascular endothelial cell monolayers | Experimental hematology | 20470859 | Positively selected peripheral blood CD19 + B cells from a healthy donor were purchased from AllCells (Emeryville, CA, USA). HBMEC were obtained from Angioproteomie (Boston, MA Save | Full Paper |
2010 | Schuhmann, NK;Pozzoli, O;Sallach, J;Huber, A;Avitabile, D;Perabo, L;Rappl, G;Capogrossi, MC;Hallek, M;Pesce, M;Büning, H; | Gene transfer into human cord blood-derived CD34(+) cells by adeno-associated viral vectors | Experimental hematology | 20447441 | After thawing, hCD34 + cells (AllCells) were plated and expanded for 3 days as described. Cells were then incubated with rAAV2sc (at a GOI of 1 × 10 4 ) for 18 hours, followed by an Save | Full Paper |
2010 | Lu, M;Zhang, W;Li, Y;Berenzon, D;Wang, X;Wang, J;Mascarenhas, J;Xu, M;Hoffman, R; | Interferon-alpha targets JAK2V617F-positive hematopoietic progenitor cells and acts through the p38 MAPK pathway | Experimental hematology | 20303384 | Objective Interferon-α (IFNα) therapy leads to hematological remissions and a reduction of the JAK2V617F allele burden in patients with polycythemia vera (PV). In this study, the Save | Full Paper |
2010 | Yourek, G;McCormick, SM;Mao, JJ;Reilly, GC; | Shear stress induces osteogenic differentiation of human mesenchymal stem cells | Regenerative medicine | 20868327 | Isolated bone marrow (AllCells, Berkeley, CA, USA) from multiple healthy human donors aged between 20 and 26 years (seven male and one female) was enriched for hMSCs using RosetteSep® (StemCell Technologies, Vancouver, BC, Canada). Bone marrow was in | Full Paper |
2010 | Barbagallo, I;Vanella, A;Peterson, SJ;Kim, DH;Tibullo, D;Giallongo, C;Vanella, L;Parrinello, N;Palumbo, GA;Di Raimondo, F;Abraham, NG;Asprinio, D; | Overexpression of heme oxygenase-1 increases human osteoblast stem cell differentiation | Journal of bone and mineral metabolism | 19924377 | Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA, USA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential Save | Full Paper |
2010 | Covey, TM;Putta, S;Cesano, A; | Single cell network profiling (SCNP): mapping drug and target interactions | Assay and drug development technologies | 20158439 | Cryopreserved, Ficoll fractionated, bone marrow samples from healthy individual were purchased from AllCells (Emeryville, CA). Cells from cryogenic storage were thawed in a 37C Save | Full Paper |
2010 | Bingaman, MG;Basu, GD;Golding, TC;Chong, SK;Lassen, AJ;Kindt, TJ;Lipinski, CA; | The autophilic anti-CD20 antibody DXL625 displays enhanced potency due to lipid raft-dependent induction of apoptosis | Anti-cancer drugs | 20216307 | blood lymphocytes (PBLs) were purified from healthy donor samples using standard Ficoll-Plaque separation. Normal human peripheral blood mononuclear cells (PBMCs) were from AllCells (Emeryville, California, USA). CD56-immunomagnetic separation beads | Full Paper |
2010 | Elliott, S;Busse, L;McCaffery, I;Rossi, J;Sinclair, A;Spahr, C;Swift, S;Begley, CG; | Identification of a sensitive anti-erythropoietin receptor monoclonal antibody allows detection of low levels of EpoR in cells | Journal of immunological methods | 19887071 | Human EpoR expressing erythroid primary cells were derived from human mobilized peripheral blood CD34+ cells (AllCells, LLC, Emeryville, CA) by culture for 6 days in StemSpan SFEM media (STEMCELLS Tech; Vancouver, BC, Canada) with 10 ng/ml IL-3, 10 n | Full Paper |
2010 | Kim, ES;Kim, JJ;Park, EJ; | Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft material | The journal of advanced prosthodontics | 21165181 | Commercially available human apheresed platelet plasmas were purchased from AllCells (Emeryville, CA, USA). Donors were restricted to males under 30 years-old for quality control. Save | Full Paper |
2010 | Shytle, DR;Tan, J;Ehrhart, J;Smith, AJ;Sanberg, CD;Sanberg, PR;Anderson, J;Bickford, PC; | Effects of blue-green algae extracts on the proliferation of human adult stem cells in vitro: a preliminary study | Medical science monitor : international medical journal of experimental and clinical research | 20037479 | For cell proliferation analysis, human bone marrow cells or human CD34+ cells (AllCells, Inc.) were cultured in 96 well plates (5×10 4 /well) containing 100 µL of complete medium (RPMI 1640 medium supplemented with 5% FCS). These cells were treated f | Full Paper |
2010 | Kawauchi, S;Terasaki, H;Katano, M;Murase, J;Masuda, Y;Tamura, T;Shimadzu, M; | Quality control and monitoring for the isolation process of mesenchymal stem cells and their differentiation into osteoblasts | Genetic testing and molecular biomarkers | 20187761 | Bone marrow samples (ABM001; AllCells, Emeryville, CA) were purchased after gaining approval of the ethical 270committee of the Olympus Corporation. Table 1 shows the ages and sexes of the donors. Skin-derived fibroblasts were also purchased from Lon | Full Paper |
2010 | Abbott, C;Huang, G;Ellison, AR;Chen, C;Arora, T;Szilvassy, SJ;Wei, P; | Mouse monoclonal antibodies against human c-Mpl and characterization for flow cytometry applications | Hybridoma (2005) | 20443702 | Primary human CD34 + cells isolated from normal bone marrow or G-CSF-mobilized peripheral blood were purchased from AllCells (Emeryville, CA). Cryopreserved cells were thawed Save | Full Paper |
2010 | Clarkson-Jones, J;Page, C;Sarda, S;Swaisland, H; | 417 Human biotransformation of olaparib (AZD2281) an oral poly(ADP-ribose) polymerase (PARP) inhibitor | European Journal of Cancer Supplements | Methods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommended by the manufacturer (StemCell Technologies). Effects of SB | Full Paper | |
2010 | Nokihara, H;Yamamoto, N;Yamada, Y;Asahina, H;Shibata, T;Seki, Y;Tamura, Y;Honda, K;Misawa, S;Tamura, T; | 415 A phase I study evaluating the safety profile and pharmacokinetics of CS-1008 (Tigatuzumab), humanized monoclonal antibody targeting death receptor 5 (DR5), in Japanese patients with advanced solid tumours | European Journal of Cancer Supplements | Methods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommended by the manufacturer (StemCell Technologies). Effects of SB | Full Paper | |
2010 | Padgett, C;Lim, J;Marschke, R;Northfelt, D;Andreopoulou, E;Hoff, D;Anderes, K;Ryckman, D;Chen, T;O'Brien, S; | 414 Clinical pharmacokinetics and pharmacodynamics of CX-4945, a novel inhibitor of protein kinase CK2: Interim report from the phase 1 clinical trial | European Journal of Cancer Supplements | that is currently in phase Ib clinical trials in AML and MDS. Methods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommen | Full Paper | |
2010 | Antognoli, A; | Rag2-/-; gammac-/-immunodeficient mice, a new preclinical model to study antitumor approaches | Thesis | CD133+ human cord blood progenitor cells (derived from single donors) purchased from ALLCELLS (Emerville, CA), or kindly provided by Professor Lemoli of the Department of Hematology and Oncologic Sciences “L. e A. Seragnoli”, University of Bologna | Full Paper | |
2010 | Tanavde, V; | Commercialization of Cord Blood | Umbilical Cord Blood | AllCells, a California-based company and Lonza are leading players in this area. BioE also sells its MLPC cell line for research and is developing the use of MLPC as a platform for Save | Full Paper | |
2010 | Nowakiewicz, M; | MST-based method for 6DOF rigid body motion planning in narrow passages | 2010 IEEE/RSJ International Conference on Intelligent Robots and Systems | construction of MST give priority to cells at levels equal or less than { by always treating their weights as lesser than weights of the other cells. 3) ALLCELLS() - Return the set of all leaf cells. 4) NEIGHBORHOOD(z) - Return the set of all cells i | Full Paper | |
2010 | Briercheck, E;Freud, A;Caligiuri, M; | Human natural killer cell development | Natural Killer Cells | Fresh human BM was donated or purchased from AllCells, LLC (Berkeley, CA) and received within 24 hours of harvest. PB leukopaks were obtained from the American Red Cross. BM Save | Full Paper | |
2010 | Ringeisen, B;Othon, C;Wu, X;Krizman, D;Darfler, M;Anders, J;Wu, P; | Biological Laser Printing (BioLP) for High Resolution Cell Deposition | Cell and Organ Printing | HUVEC were purchased from Allcells, LLC (Emeryville, California) and cultured according to the protocol provided. HUSMC were purchased from ATCC and cultured according to the protocol provided by the supplier. In both cases, per ml of media is fortif | Full Paper | |
2010 | Leskov, I; | Modeling hematologic malignancies and their treatment in humanized mice | Thesis | Purified CD133* human umbilical cord hematopoietic stem cells (HSCs) were purchased from AllCells or from the National Disease Research Interchange (NDRI). Alternatively, CD133* cells were purified from fresh umbilical cord blood purchased from NDRI, | Full Paper | |
2010 | Chao, MP; | Modulating Phagocytosis: Therapeutic Targeting of the Anti-Phagocytic Signal CD47 in Human Malignancies | Thesis | Human Samples Normal human bone marrow mononuclear cells were purchased from AllCells Inc. (Emeryville, CA, USA). Human AML samples (Figure 1B) were obtained from patients at the Stanford Medical Center with informed consent, according to an IRB-appr | Full Paper | |
2010 | Lee, CH; | Connective tissue growth factor (CCN2/CTGF)-induced fibrogenesis: Biology, bioengineering and therapeutics | Thesis | Human mesenchymal stem cells (hMSCs) were isolated from commercially available fresh bone marrow samples of anonymous adult donors (AllCells, Berkeley, CA). Purification of hMSCs was accomplished by centrifugation through a density gradient (Ficoll-P | Full Paper | |
2010 | Collins, JM; | Stem cells are affected by physical and chemical components of the microenvironmental niche | Thesis | xperiments were performed on P2 hMSCs obtained from 2 separate donors. Additionally, some experiments were repeated with P2 hMSCs isolated from whole marrow (AllCells, Berkely, CA) as described previously (Alhadlaq et al., 2005) | Full Paper | |
2010 | Scaife, MD; | Truncated Cell Surface Markers Fused with Mutant Human Tmpk: Versatile Cell Fate Control Safety Cassettes for Lentiviral Vector Mediated Correction of Fabry Disease | Thesis | Human CD34+ cells were purchased from AllCells and cultured in Stem Span SFEM expansion medium (Stem Cell Technologies) and supplemented with 10 ng/ml human interleukin-6 (IL-6), 100 ng/ml human Flt3 Ligand (Flt3-L), 100 ng/ml human thrombopoietin (T | Full Paper | |
2010 | Van Craenenbroeck, E; | Exercise in chronic heart failure focus on endothelial repair | Thesis | typesmost likely have important contributions to the initiation, continuation and regulation ofvascular homeostasis In the following, the term EPC is used for general description of allcells Save | Full Paper | |
2010 | Jangam, SR; | A PCR based HIV-1 test for point-of-care application | Thesis | It was observed that there was a saturation effect being observed at total WBC 500,000 cells. To further confirm this observation, leukocyte depleted blood was spiked with different amounts of PBMC (ALLCELLS, Emeryville, CA) in the range of 46,000-2 | Full Paper | |
2010 | Sofia, MJ;Bao, D;Chang, W;Du, J;Nagarathnam, D;Rachakonda, S;Reddy, PG;Ross, BS;Wang, P;Zhang, HR;Bansal, S;Espiritu, C;Keilman, M;Lam, AM;Steuer, HM;Niu, C;Otto, MJ;Furman, PA; | Discovery of a β-d-2'-deoxy-2'-α-fluoro-2'-β-C-methyluridine nucleotide prodrug (PSI-7977) for the treatment of hepatitis C virus | Journal of medicinal chemistry | 20845908 | We acknowledge the following individuals and organizations for their contributions to the work described in this manuscript: Wuxi AppTec; ReachBio LLC; BASi; and Dr. Patrick Carroll, University of Pennsylvania | Full Paper |
2010 | Lam, AM;Murakami, E;Espiritu, C;Steuer, HM;Niu, C;Keilman, M;Bao, H;Zennou, V;Bourne, N;Julander, JG;Morrey, JD;Smee, DF;Frick, DN;Heck, JA;Wang, P;Nagarathnam, D;Ross, BS;Sofia, MJ;Otto, MJ;Furman, PA; | PSI-7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype inhibitor of hepatitis C virus replication | Antimicrobial agents and chemotherapy | 20516278 | Human bone marrow progenitor cell toxicity assays were performed by Emer Clarke at ReachBio LLC (Seattle, WA) | Full Paper |
2010 | Maloney, JM;Nikova, D;Lautenschläger, F;Clarke, E;Langer, R;Guck, J;Van Vliet, KJ; | Mesenchymal stem cell mechanics from the attached to the suspended state | Biophysical journal | 20959088 | artment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts ¶Department of Physics, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom ‖ReachBio, Seattle, Washington ∗Corresponding author k | Full Paper |
2009 | Nemunaitis, J;Clayman, G;Agarwala, SS;Hrushesky, W;Wells, JR;Moore, C;Hamm, J;Yoo, G;Baselga, J;Murphy, BA;Menander, KA;Licato, LL;Chada, S;Gibbons, RD;Olivier, M;Hainaut, P;Roth, JA;Sobol, RE;Goodwin, WJ; | Biomarkers Predict p53 Gene Therapy Efficacy in Recurrent Squamous Cell Carcinoma of the Head and Neck | Clin. Cancer Res. | 19996201 | iplex primers (Affymetrix, Inc.). The resultant PCR products were analyzed using the Affymetrix p53 GeneChip, which encompasses all coding exons, 2 to 11, as well as immediate flanking regions. Confirmation of the results was carried out by sequencin | Full Paper |
2009 | Mori, F;Oda, N;Sakuragi, M;Sakakibara, F;Kiniwa, M;Miyoshi, K; | New histopathological experimental model for benign prostatic hyperplasia: stromal hyperplasia in rats | J. Urol. | 19095262 | All sections were developed by the avidin-biotin complex method. Sections were also stained with hematoxylin and eosin, and Masson's trichrome. Human BPH tissue samples (Folio Biosciences, Columbus, Ohio) were also stained. Histological Analysis | Full Paper |
2009 | Fazzone, W;Wilson, PM;Labonte, MJ;Lenz, HJ;Ladner, RD; | Histone deacetylase inhibitors suppress thymidylate synthase gene expression and synergize with the fluoropyrimidines in colon cancer cells | Int. J. Cancer | 19384949 | liquid nitrogen or fixed in 10% formalin. Formalin‐fixed tumor specimens were paraffin‐embedded and subsequently analyzed by immunohistochemistry (IHC) (QualTek Laboratories, Newtown, PA). IHC for TS was conducted as | Full Paper |
2009 | Donkor, J;Zhang, P;Wong, S;O'Loughlin, L;Dewald, J;Kok, BP;Brindley, DN;Reue, K; | A conserved serine residue is required for the phosphatidate phosphatase activity but not the transcriptional coactivator functions of lipin-1 and lipin-2 | J. Biol. Chem. | 19717560 | Invitrogen). Plasmids were sequences to select templates for sense and antisense probe generation and linearized by digestion with BamHI. In situ hybridization was performed using the services of Phylogeny, Inc. (Columbus, OH) | Full Paper |
2009 | Maxwell, SA;Li, Z;Jaye, D;Jaya, D;Ballard, S;Ferrell, J;Fu, H; | 14-3-3zeta mediates resistance of diffuse large B cell lymphoma to an anthracycline-based chemotherapeutic regimen | J. Biol. Chem. | 19525224 | 3′. Preparation of DLBCL Tissue Extracts for Western Blotting. Normal and DLBCL lymph node tissues were procured through Folio Biosciences. Frozen tissue was ground to a powder in liquid nitrogen in a pestle and mortar | Full Paper |
2009 | Deroo, BJ;Rodriguez, KF;Couse, JF;Hamilton, KJ;Collins, JB;Grissom, SF;Korach, KS; | Estrogen receptor beta is required for optimal cAMP production in mouse granulosa cells | Mol. Endocrinol. | 19324971 | Mice were treated with vehicle or PMSG for 48 h, after which the ovaries were removed and snap frozen in 2-methylbutane (isopentane) and then stored at −70 C. In situ hybridization analysis for the detection of Lhcgr in whole ovaries was then carried | Full Paper |
2009 | Cunningham, D;Spychala, K;McLarren, KW;Garza, LA;Boerkoel, CF;Herman, GE; | Developmental expression pattern of the cholesterogenic enzyme NSDHL and negative selection of NSDHL-deficient cells in the heterozygous Bpa(1H)/+ mouse | Mol. Genet. Metab. | 19631568 | A darkfield view of a similar section hybridized with a control 35S-labeled sense Nsdhl RNA probe, showing little or no nonspecific background hybridization. (D) A brightfield view of a lateral sagittal section of the E15.5 embryo that includes the e | Full Paper |
2009 | Chen, LM;Verity, NJ;Chai, KX; | Loss of prostasin (PRSS8) in human bladder transitional cell carcinoma cell lines is associated with epithelial-mesenchymal transition (EMT) | BMC Cancer | 19849847 | (Hayward, CA). A Bladder Carcinoma TMA (ARY-HH0087) containing 80 tissue cores, each at 1.5 mm in diameter; with 40 cancer tissues and 40 matching or independent non-cancerous tissues was obtained from Folio Biosciences (Columbus, OH) | Full Paper |
2009 | Binkert, CA;Drooz, AT;Caridi, JG;Sands, MJ;Bjarnason, H;Lynch, FC;Rilling, WS;Zambuto, DA;Stavropoulos, SW;Venbrux, AC;Kaufman, JA; | Technical success and safety of retrieval of the G2 filter in a prospective, multicenter study | J Vasc Interv Radiol | 19875062 | Full Paper | |
2009 | Lynch, FC; | Balloon-assisted removal of tilted inferior vena cava filters with embedded tips | J Vasc Interv Radiol | 19729132 | Full Paper | |
2009 | Lynch, FC;Kekulawela, S; | Removal of the G2 filter: differences between implantation times greater and less than 180 days | J Vasc Interv Radiol | 19640738 | Full Paper | |
2009 | Cantwell, CP;Pennypacker, J;Singh, H;Scorza, LB;Waybill, PN;Lynch, FC; | Comparison of the recovery and G2 filter as retrievable inferior vena cava filters | J Vasc Interv Radiol | 19640733 | Full Paper | |
2009 | Choi, KD;Vodyanik, MA;Slukvin, II; | Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors | The Journal of clinical investigation | 19726877 | CD1a+ DCs, or LCs were cocultured with 1 × 105 adult peripheral (AllCells) or cord blood (Cambrex) mononuclear cells in RPMI 1640 medium (Invitrogen) containing 5% human AB serum (Sigma-Aldrich) in 96-well flat-bottom plates. At 6 days of culture, th | Full Paper |
2009 | Pfeffer, BA;Bernstein, SA;Bartels, SP; | Preservation of structure and immunoreactivity at the vitreoretinal interface of the rabbit eye | Graefes Arch. Clin. Exp. Ophthalmol. | 19020891 | Presented in part at the 2006 meeting of The Association for Research in Vision and Ophthalmology, Fort Lauderdale, Florida. This study was supported by Bausch & Lomb, Inc., with whom QualTek Molecular Laboratories has a contractual relationship | Full Paper |
2009 | Petrie, KR;Schenk, T;Chornet, MB;Howell, L;Dick, JE; | Modulation of Histone H3K4 and H3K27 Methylation Levels Via Pharmacological Inhibition of LSD1 and Degradation of the EZH2-Containing Polycomb Repressive Complex 2 Stimulates ATRA-Mediated Differentiation of AML Cells | Conference | Flow cytometric detection of apoptosis among normal mononuclear cells obtained from the bone marrow of healthy volunteers (AllCells, LLC) by phycoerythrin (PE)conjugated Annexin- Save | Full Paper | |
2009 | Muchamuel, T;Basler, M;Aujay, MA;Suzuki, E;Kalim, KW;Lauer, C;Sylvain, C;Ring, ER;Shields, J;Jiang, J;Shwonek, P;Parlati, F;Demo, SD;Bennett, MK;Kirk, CJ;Groettrup, M; | A selective inhibitor of the immunoproteasome subunit LMP7 blocks cytokine production and attenuates progression of experimental arthritis | Nature medicine | 19525961 | We purchased human PBMCs from normal healthy volunteers from AllCells, and we obtained PBMCs from individuals with rheumatoid arthritis (who had given informed consent) from a Save | Full Paper |
2009 | Majeti, R;Chao, MP;Alizadeh, AA;Pang, WW;Jaiswal, S;Gibbs, KD;van Rooijen, N;Weissman, IL; | CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cells | Cell | 19632179 | Acute myeloid leukemia (AML) is organized as a cellular hierarchy initiated and maintained by a subset of self-renewing leukemia stem cells (LSC). We hypothesized that increased Save | Full Paper |
2009 | Schmitt, N;Morita, R;Bourdery, L;Bentebibel, SE;Zurawski, SM;Banchereau, J;Ueno, H; | Human dendritic cells induce the differentiation of interleukin-21-producing T follicular helper-like cells through interleukin-12 | Immunity | 19592276 | sorting with FACSAria (BD) as CCR7+ CD8- CD56- HLA-DR- CD45RA+ CD4+ cells. Memory CD4+ T cells were directly sorted from PBMCs as CD8- CD56- CD45RA- CD4+ cells. Cord blood naïve CD4 T cells were sorted from frozen cells (AllCells) as CCR7+ CD8- CD56- | Full Paper |
2009 | Wang, Y;Sul, HS; | Pref-1 regulates mesenchymal cell commitment and differentiation through Sox9 | Cell metabolism | 19254573 | Human-derived marrow stromal cells (MSC) and MSC media were purchased from Allcells. Sox9 target-specific siRNA (sc-36534) (Santa Cruz) and the control siRNA (sc-36869) were Save | Full Paper |
2009 | Trifari, S;Kaplan, CD;Tran, EH;Crellin, NK;Spits, H; | Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells | Nature immunology | 19578368 | Naive CD4 + T cells were obtained from human cord blood (AllCells) with a Naive CD4 + T cell Isolation Kit II (Miltenyi), followed by sorting of the CD4 + CD45RA + CD45RO − Save | Full Paper |
2009 | Ye, Z;Zhan, H;Mali, P;Dowey, S;Williams, DM;Jang, YY;Dang, CV;Spivak, JL;Moliterno, AR;Cheng, L; | Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disorders | Blood | 19797525 | Isolated PB CD34 + cells after G-CSF mobilization were purchased from AllCells and used as a normal control for analyzing gene expression. Four classic retroviral vectors pMXs-Oct4, Save | Full Paper |
2009 | Parlati, F;Lee, SJ;Aujay, M;Suzuki, E;Levitsky, K;Lorens, JB;Micklem, DR;Ruurs, P;Sylvain, C;Lu, Y;Shenk, KD;Bennett, MK; | Carfilzomib can induce tumor cell death through selective inhibition of the chymotrypsin-like activity of the proteasome | Blood | 19671918 | Rosen's laboratory at Northwestern University. CD138+ cells and peripheral blood mononuclear cells (PBMCs) were purchased from Allcells. The purity of CD138+ cells was greater than 90% as determined by the supplier. HS-Sultan-GFPu cell lines were gen | Full Paper |
2009 | Figueroa, ME;Skrabanek, L;Li, Y;Jiemjit, A;Fandy, TE;Paietta, E;Fernandez, H;Tallman, MS;Greally, JM;Carraway, H;Licht, JD;Gore, SD;Melnick, A; | MDS and secondary AML display unique patterns and abundance of aberrant DNA methylation | Blood | 19652201 | Support Laboratory, Cincinnati Children's Hospital, and 4 from Allcells. Matched CD34+ and CD34− fractions were obtained from an additional 4 healthy donors through the Stem Cell and Xenograft Core, University of Pennsylvania School of Medicine | Full Paper |
2009 | Hasstedt, SJ;Bezemer, ID;Callas, PW;Vossen, CY;Trotman, W;Hebbel, RP;Demers, C;Rosendaal, FR;Bovill, EG; | Cell adhesion molecule 1: a novel risk factor for venous thrombosis | Blood | 19643986 | cover slips coated with gelatin (Allcells Inc) under sterile conditions at 37C, 5% CO 2 in HUVEC Basal Medium containing HUVEC Basal Medium Supplement (Allcells Inc). Cells were Save | Full Paper |
2009 | Shi, P;Lai, R;Lin, Q;Iqbal, AS;Young, LC;Kwak, LW;Ford, RJ;Amin, HM; | IGF-IR tyrosine kinase interacts with NPM-ALK oncogene to induce survival of T-cell ALK+ anaplastic large-cell lymphoma cells | Blood | 19423729 | Type I insulin-like growth factor receptor (IGF-IR) tyrosine kinase plays important roles in the pathogenesis of several malignancies. Although it promotes the growth of stimulated Save | Full Paper |
2009 | Loh, YH;Agarwal, S;Park, IH;Urbach, A;Huo, H;Heffner, GC;Kim, K;Miller, JD;Ng, K;Daley, GQ; | Generation of induced pluripotent stem cells from human blood | Blood | 19299331 | obtained from Allcells (Emeryville, CA) were collected from a 26-year-old male donor, and CD34+ cells (mPB014F) were isolated. Generation of CD34 iPS cells was performed using a protocol modified from previously published methods.9 | Full Paper |
2009 | Nahimana, A;Attinger, A;Aubry, D;Greaney, P;Ireson, C;Thougaard, AV;Tjørnelund, J;Dawson, KM;Dupuis, M;Duchosal, MA; | The NAD biosynthesis inhibitor APO866 has potent antitumor activity against hematologic malignancies | Blood | 19196867 | APO866 inhibits nicotinamide phosphoribosyltransferase (NMPRTase), a key enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis from the natural precursor Save | Full Paper |
2009 | Nardi, V;Naveiras, O;Azam, M;Daley, GQ; | ICSBP-mediated immune protection against BCR-ABL-induced leukemia requires the CCL6 and CCL9 chemokines | Blood | 19171873 | eripheral blood mononuclear cells from healthy volunteers were purchased from AllCells (Emeryville, CA) and maintained in complete RPMI medium. Murine and human cells were treated with IFN alpha 2A or IFN beta (R&D Systems | Full Paper |
2009 | Figueroa, ME;Wouters, BJ;Skrabanek, L;Glass, J;Li, Y;Erpelinck-Verschueren, CA;Langerak, AW;Löwenberg, B;Fazzari, M;Greally, JM;Valk, PJ;Melnick, A;Delwel, R; | Genome-wide epigenetic analysis delineates a biologically distinct immature acute leukemia with myeloid/T-lymphoid features | Blood | 19168792 | 4 acquired from the Translational Trials Development and Support Laboratory, Cincinnati Children's Hospital (Cincinnati, OH) and 4 purchased from Allcells (Emeryville, CA). This research was approved by the institutional review boards at Albert Einst | Full Paper |
2009 | Chen, H;Campbell, RA;Chang, Y;Li, M;Wang, CS;Li, J;Sanchez, E;Share, M;Steinberg, J;Berenson, A;Shalitin, D;Zeng, Z;Gui, D;Perez-Pinera, P;Berenson, RJ;Said, J;Bonavida, B;Deuel, TF;Berenson, JR; | Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: a novel mechanism of tumor-induced vasculogenesis | Blood | 19060246 | The human RPMI8226 and U266 MM cell lines and monocyte (THP-1) and myelomonocytic (U937) cell lines were obtained from ATCC (Manassas, VA). Normal human BM was purchased from Allcells (Berkeley, CA). Highly purified human T cells from healthy donors | Full Paper |
2009 | Luo, XM;Maarschalk, E;O'Connell, RM;Wang, P;Yang, L;Baltimore, D; | Engineering human hematopoietic stem/progenitor cells to produce a broadly neutralizing anti-HIV antibody after in vitro maturation to human B lymphocytes | Blood | 19059876 | 90 minutes at 50 000 × g. Human cord blood CD34+ HSPCs were purchased from AllCells (Emeryville, CA) and maintained for less than 24 hours before infection in Iscove modified Dulbecco medium containing 10% fetal bovine serum and cytokines. The cytoki | Full Paper |
2009 | Joshi, I;Minter, LM;Telfer, J;Demarest, RM;Capobianco, AJ;Aster, JC;Sicinski, P;Fauq, A;Golde, TE;Osborne, BA; | Notch signaling mediates G1/S cell-cycle progression in T cells via cyclin D3 and its dependent kinases | Blood | 19001083 | Notch signaling is critical for the regulation of differentiation, proliferation and apoptosis in many cell types. Notch receptors and various Notch ligands have been shown to have a Save | Full Paper |
2009 | Bedi, R;Du, J;Sharma, AK;Gomes, I;Ackerman, SJ; | Human C/EBP-epsilon activator and repressor isoforms differentially reprogram myeloid lineage commitment and differentiation | Blood | 18832658 | Cord blood (CB) CD34+ cells ( 97% pure) purchased from AllCells (Emeryville, CA) were prestimulated for 18 hours at 106 cells/mL in presence of 100 ng/mL recombinant human stem cell factor (rh-SCF), recombinant human fms-like tyrosine kinase 3 ligand | Full Paper |
2009 | Oda, M;Glass, JL;Thompson, RF;Mo, Y;Olivier, EN;Figueroa, ME;Selzer, RR;Richmond, TA;Zhang, X;Dannenberg, L;Green, RD;Melnick, A;Hatchwell, E;Bouhassira, EE;Verma, A;Suzuki, M;Greally, JM; | High-resolution genome-wide cytosine methylation profiling with simultaneous copy number analysis and optimization for limited cell numbers | Nucleic acids research | 19386619 | One million fresh CD34 + bone marrow progenitors were purchased from Allcells (Emerville, CA). DNA was extracted from these cells and checked for purity and amount by Save | Full Paper |
2009 | O'Brien, JJ;Baglole, CJ;Garcia-Bates, TM;Blumberg, N;Francis, CW;Phipps, RP; | 15-deoxy-Delta12,14 prostaglandin J2-induced heme oxygenase-1 in megakaryocytes regulates thrombopoiesis | Journal of thrombosis and haemostasis : JTH | 18983509 | Human CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5×105 cells per well in a 12-well plate and cultured in serum-free medium as previously described (19) and supplemented with 100 ng/mL of recombinant hu | Full Paper |
2009 | Liu, PC;Caulder, E;Li, J;Waeltz, P;Margulis, A;Wynn, R;Becker-Pasha, M;Li, Y;Crowgey, E;Hollis, G;Haley, P;Sparks, RB;Combs, AP;Rodgers, JD;Burn, TC;Vaddi, K;Fridman, JS; | Combined inhibition of Janus kinase 1/2 for the treatment of JAK2V617F-driven neoplasms: selective effects on mutant cells and improvements in measures of disease severity | Clinical cancer research : an official journal of the American Association for Cancer Research | 19887489 | Mononuclear cells were isolated from peripheral blood samples from patients with PV or normal control individuals by centrifugation through Ficoll (Allcells, Inc.). Colony forming assays Save | Full Paper |
2009 | Cardarelli, PM;Moldovan-Loomis, MC;Preston, B;Black, A;Passmore, D;Chen, TH;Chen, S;Liu, J;Kuhne, MR;Srinivasan, M;Assad, A;Witte, A;Graziano, RF;King, DJ; | In vitro and in vivo characterization of MDX-1401 for therapy of malignant lymphoma | Clinical cancer research : an official journal of the American Association for Cancer Research | 19401346 | ADCC assay. Human peripheral blood mononuclear effector cells were purified from either heparinized whole blood (AllCells) or buffy coat (Stanford Blood Center) by standard Ficoll-Paque separation. Cells (2 × 106) were washed in PBS and sent for geno | Full Paper |
2009 | Zhong, C;Qu, X;Tan, M;Meng, YG;Ferrara, N; | Characterization and regulation of bv8 in human blood cells | Clinical cancer research : an official journal of the American Association for Cancer Research | 19336519 | Isolation of whole human bone marrow cells from fresh bone marrow collections. Fresh bone marrow samples were obtained from ALLCELLS. The bone marrow was first diluted with 10% fetal bovine serum-containing DMEM and then passed through a 40-μm cell s | Full Paper |
2009 | Ara, T;Song, L;Shimada, H;Keshelava, N;Russell, HV;Metelitsa, LS;Groshen, SG;Seeger, RC;DeClerck, YA; | Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells | Cancer research | 19118018 | Human BMSC were either purchased from ALLCells LLC or obtained from bone marrow samples of patients with neuroblastoma enrolled by the Children's Oncology Group. These Save | Full Paper |
2009 | Senyuk, V;Rinaldi, CR;Li, D;Cattaneo, F;Stojanovic, A;Pane, F;Du, X;Mahmud, N;Dickstein, J;Nucifora, G; | Consistent up-regulation of Stat3 Independently of Jak2 mutations in a new murine model of essential thrombocythemia | Cancer research | 19118011 | Mononuclear cells were separated from the BM aspirates by Ficoll-Hypaque centrifugation. The samples were obtained according to guidelines established by the University of Illinois at Chicago Institutional Review Board. Healthy donors’ BM cells were | Full Paper |
2009 | Kim, YC;Wu, Q;Chen, J;Xuan, Z;Jung, YC;Zhang, MQ;Rowley, JD;Wang, SM; | The transcriptome of human CD34+ hematopoietic stem-progenitor cells | Proceedings of the National Academy of Sciences of the United States of America | 19416867 | De Novo Collection of CD34+ 3′ ESTs. Bone marrow CD34+ cells of 3 healthy donors were purchased from AllCells. 3′ ESTs were collected by using the GLGI method (18, 19). A total of 10,000 CD34+ SAGE tags identified in a previous study (12) were select | Full Paper |
2009 | Majeti, R;Becker, MW;Tian, Q;Lee, TL;Yan, X;Liu, R;Chiang, JH;Hood, L;Clarke, MF;Weissman, IL; | Dysregulated gene expression networks in human acute myelogenous leukemia stem cells | Proceedings of the National Academy of Sciences of the United States of America | 19218430 | Normal human bone marrow mononuclear cells were either purchased from AllCells or obtained from National Marrow Donor Program discarded filter units. For AML specimens, peripheral blood and/or bone marrow was obtained following informed consent at th | Full Paper |
2009 | Bernard, JJ;Seweryniak, KE;Koniski, AD;Spinelli, SL;Blumberg, N;Francis, CW;Taubman, MB;Palis, J;Phipps, RP; | Foxp3 regulates megakaryopoiesis and platelet function | Arteriosclerosis, thrombosis, and vascular biology | 19661482 | Human CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5×105 cells per well in a 12-well plate and cultured in serum-free medium as previously described 12 and supplemented with 100 ng/mL of recombinant huma | Full Paper |
2009 | Jekle, A;Chow, E;Kopetzki, E;Ji, C;Yan, MJ;Nguyen, R;Sankuratri, S;Cammack, N;Heilek, G; | CD4-BFFI: a novel, bifunctional HIV-1 entry inhibitor with high and broad antiviral potency | Antiviral research | 19559732 | obtained from ATCC, Manassas, VA. Human PBMC were obtained from AllCells (Emeryville, CA), stimulated for one day in PBMC media (RPMI-1640 media containing 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 2 mM l-glutamine, 1 mM sodium-pyruva | Full Paper |
2009 | Nakahara, K;Wakasa-Morimoto, C;Kobayashi, M;Miki, S;Noshi, T;Seki, T;Kanamori-Koyama, M;Kawauchi, S;Suyama, A;Fujishita, T;Yoshinaga, T;Garvey, EP;Johns, BA;Foster, SA;Underwood, MR;Sato, A;Fujiwara, T; | Secondary mutations in viruses resistant to HIV-1 integrase inhibitors that restore viral infectivity and replication kinetics | Antiviral research | 19027039 | Passage of HIV-1 in the presence of integrase inhibitors (INIs) generates resistant viruses that have mutations in the integrase region. Integrase-resistant mutations Q148K and Q148R Save | Full Paper |
2009 | Strickland, LA;Ross, J;Williams, S;Ross, S;Romero, M;Spencer, S;Erickson, R;Sutcliffe, J;Verbeke, C;Polakis, P;van Bruggen, N;Koeppen, H; | Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinoma | The Journal of pathology | 19334050 | consenting donors; bone marrow samples and purified CD34+ cells (AllCells Inc., Berkeley, CA, USA) were received within 5 (unfractionated samples) or 24 (fractionated samples) h of collection. PC3 (CRL-1435) and HPAFII (CRL-2119) cells were obtained | Full Paper |
2009 | Leleu, X;Hunter, ZR;Xu, L;Roccaro, AM;Moreau, AS;Santos, DD;Hatjiharissi, E;Bakthavachalam, V;Adamia, S;Ho, AW;Soumerai, J;Patterson, CJ;Manning, RJ;Hamilton, S;Verselis, S;Fox, E;Carrasco, R;Ghobrial, IM;Treon, SP; | Expression of regulatory genes for lymphoplasmacytic cell differentiation in Waldenstrom Macroglobulinemia | British journal of haematology | 19220283 | years and the male/female ratio was 1·6. All WM patients were previously untreated. Table I summarizes the characteristics of the patients. Bone marrow aspirates from healthy donors were used in comparison (AllCells LLC, Berkeley, CA, USA) | Full Paper |
2009 | Krupa, A;Fudala, R;Stankowska, D;Loyd, T;Allen, TC;Matthay, MA;Gryczynski, Z;Gryczynski, I;Mettikolla, YV;Kurdowska, AK; | Anti-chemokine autoantibody:chemokine immune complexes activate endothelial cells via IgG receptors | American journal of respiratory cell and molecular biology | 19109244 | HUVECs were purchased from Cambrex (Walkersville, MD) or Allcells (Berkeley, CA), and cultured in endothelial basal medium containing endothelial growth factor, hydrocortisone, gentamicin, amphoreticin B, bovine brain extract, heparin, and FBS (EGM-M | Full Paper |
2009 | Lee, JH;Ort, T;Ma, K;Picha, K;Carton, J;Marsters, PA;Lohmander, LS;Baribaud, F;Song, XY;Blake, S; | Resistin is elevated following traumatic joint injury and causes matrix degradation and release of inflammatory cytokines from articular cartilage in vitro | Osteoarthritis and cartilage | 19095472 | Human primary monocytes isolated from three normal healthy volunteers were purchased from AllCells, LLC (Emeryville, CA). The cells were treated with recombinant human resistin (Peprotech, Inc, Rocky Hill, NJ; endotoxin level was measured as 0.9 EU/m | Full Paper |
2009 | Hashimoto, S;Nishiyama, T;Hayashi, S;Takebe, K;Kanzaki, N;Fujishiro, T;Kuroda, R;Kurosaka, M; | 224 THE FUNCTION OF p53 ON HUMAN CHONDROCYTE APOPTOSIS IN RESPONSE TO SHEAR STRESS | Osteoarthritis and Cartilage | ated by enzymatic digestion of bone from patients undergoing knee endoprothesis due to arthrosis of the knee joint. Human macrophages were differentiated from CD14+ peripheral blood monocytes (Allcells) by treatment with macrophage colony | Full Paper | |
2009 | Peterson, SJ;Kim, DH;Li, M;Positano, V;Vanella, L;Rodella, LF;Piccolomini, F;Puri, N;Gastaldelli, A;Kusmic, C;L'Abbate, A;Abraham, NG; | The L-4F mimetic peptide prevents insulin resistance through increased levels of HO-1, pAMPK, and pAKT in obese mice | Journal of lipid research | 19224872 | at various time points (0-120 min) for measurement of blood glucose levels. Human bone marrow-derived mesenchymal stem cells and adipocytes Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing the cells, | Full Paper |
2009 | Patel, E;Wang, B;Lien, L;Wang, Y;Yang, LJ;Moreb, JS;Chang, LJ; | Diverse T-cell differentiation potentials of human fetal thymus, fetal liver, cord blood and adult bone marrow CD34 cells on lentiviral Delta-like-1-modified mouse stromal cells | Immunology | 19740310 | Invitrogen, Carlsbad, CA) supplemented with 20% fetal bovine serum and 1% penicillin-streptomycin. The adult BM and CB CD34+ cells were purchased from AllCells, LLC (Emeryville, CA), and Cambrex Corp. (Baltimore, MD) Aborted fetal liver and thymus ti | Full Paper |
2009 | Sutherland, MK;Yu, C;Lewis, TS;Miyamoto, JB;Morris-Tilden, CA;Jonas, M;Sutherland, J;Nesterova, A;Gerber, HP;Sievers, EL;Grewal, IS;Law, CL; | Anti-leukemic activity of lintuzumab (SGN-33) in preclinical models of acute myeloid leukemia | mAbs | 20065652 | Primary normal human monocytes and primary AML patient bone marrow samples were obtained from AllCells (Emeryville, CA). Fluorochrome-conjugated antibodies against human Save | Full Paper |
2009 | Nan, JL;Li, JJ;He, JG; | C-reactive protein decreases interleukin-8 production in human endothelial progenitor cells by inhibition of p38 MAPK pathway | Chinese medical journal | 19781372 | Theabilityto modulate the antigenic pbenotype in response to the environmentiscommon to allcells.ECsmay regulate 1evelsofvascularendothelialgrowthfactorreceptor一2 in response to Save | Full Paper |
2009 | Patterson, ST;Li, J;Kang, JA;Wickrema, A;Williams, DB;Reithmeier, RA; | Loss of specific chaperones involved in membrane glycoprotein biosynthesis during the maturation of human erythroid progenitor cells | The Journal of biological chemistry | 19258325 | hematopoietic progenitors were either purchased from Stem Cell Technologies (mPB015F) or initially isolated from growth factor-mobilized peripheral blood (purchased from AllCells, Save | Full Paper |
2009 | Ji, C;Kopetzki, E;Jekle, A;Stubenrauch, KG;Liu, X;Zhang, J;Rao, E;Schlothauer, T;Fischer, S;Cammack, N;Heilek, G;Ries, S;Sankuratri, S; | CD4-anchoring HIV-1 fusion inhibitor with enhanced potency and in vivo stability | The Journal of biological chemistry | 19097993 | In this study, we describe a novel CD4-targeting bifunctional human immunodeficiency virus (HIV-1) fusion inhibitor (CD4-BFFI) that blocks HIV-1 entry by inhibiting both HIV-1 Save | Full Paper |
2009 | Hirschler-Laszkiewicz, I;Tong, Q;Conrad, K;Zhang, W;Flint, WW;Barber, AJ;Barber, DL;Cheung, JY;Miller, BA; | TRPC3 activation by erythropoietin is modulated by TRPC6 | The Journal of biological chemistry | 19074769 | Human erythroid progenitors were also cultured using a two-phase liquid culture system (44, 45). Cells were harvested at day 8 of phase II and were predominantly proerythroblasts or basophilic normoblasts. CD34+ cells were purchased from AllCells, LL | Full Paper |
2009 | Zhang, Q;Shi, S;Liu, Y;Uyanne, J;Shi, Y;Shi, S;Le, AD; | Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitis | Journal of immunology (Baltimore, Md. : 1950) | 19923445 | Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells and cultured with α-MEM supplemented with 10% FBS, 100 μM l-ascorbic acid-2-phosphate, 2 mM l-glutamine, 100 U/ml penicillin, and 100 μg/ml strept | Full Paper |
2009 | Parrish, YK;Baez, I;Milford, TA;Benitez, A;Galloway, N;Rogerio, JW;Sahakian, E;Kagoda, M;Huang, G;Hao, QL;Sevilla, Y;Barsky, LW;Zielinska, E;Price, MA;Wall, NR;Dovat, S;Payne, KJ; | IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrow | Journal of immunology (Baltimore, Md. : 1950) | 19299724 | CB was collected according to guidelines approved by the Institutional Review Boards at Childrens Hospital Los Angeles and Loma Linda University. Adult human BM was purchased from AllCells, LLC, Berkeley, CA; the National Disease Research Interchange | Full Paper |
2009 | Vendel, AC;Calemine-Fenaux, J;Izrael-Tomasevic, A;Chauhan, V;Arnott, D;Eaton, DL; | B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK | Journal of immunology (Baltimore, Md. : 1950) | 19155498 | Tonsil tissue (AllCells) was dissociated and a single-cell suspension was stained with anti-CD19, anti-IgD, and anti-CD38. Naive tonsillar B cells were identified as CD19 + IgD + CD38 Save | Full Paper |
2009 | Panetta, NJ;Gupta, DM;Kwan, MD;Wan, DC;Commons, GW;Longaker, MT; | Tissue harvest by means of suction-assisted or third-generation ultrasound-assisted lipoaspiration has no effect on osteogenic potential of human adipose-derived stromal cells | Plastic and reconstructive surgery | 19568046 | Commercially available bone marrow mesenchymal stem cells (AllCells), subjected to similar experimental osteogenic differentiation conditions, demonstrated significantly less staining Save | Full Paper |
2009 | Weigel-Van Aken, KA; | Pharmacological activation of guanine nucleotide exchange factors for the small GTPase Rap1 recruits high-affinity beta1 integrins as coreceptors for parvovirus B19: improved ex vivo gene transfer to human erythroid progenitor cells | Human gene therapy | 19702438 | Human bone marrow-derived CD36 + erythroid progenitor cells were purchased from AllCells (Emeryville, CA). Cells were cultured overnight in serum-free medium containing stem cell Save | Full Paper |
2009 | Zinckgraf, JW;Sposato, M;Zielinski, V;Powell, D;Treanor, JJ;von Hofe, E; | Identification of HLA class II H5N1 hemagglutinin epitopes following subvirion influenza A (H5N1) vaccination | Vaccine | 19596415 | Since we did not have access to pre-vaccine samples, eight random naive donor PBMC samples (AllCells, Emeryville, CA) were utilized to assess potential cross-reactive T cell Save | Full Paper |
2009 | Morizono, K;Pariente, N;Xie, Y;Chen, IS; | Redirecting lentiviral vectors by insertion of integrin-tageting peptides into envelope proteins | The journal of gene medicine | 19434609 | Human umbilical vein endothelial cells (HUVEC) were purchased from AllCells (Emeryville, CA, USA) and Lonza (Walkersville, MD, USA). HUVEC were cultured in EBM-2 (Lonza) supplemented with EGM-2 singlequots (Lonza). When HUVEC started growing slowly, | Full Paper |
2009 | Liang, M;Pariente, N;Morizono, K;Chen, IS; | Targeted transduction of CD34+ hematopoietic progenitor cells in nonpurified human mobilized peripheral blood mononuclear cells | The journal of gene medicine | 19152374 | Cells binding with the anti-CD34 antibody were separated by a MACS machine (Miltenyi Biotec). Human primary PBMCs were isolated from leukopacks, Ficoll purified and cultured in RPMI with 20% FCS (Omega Scientific, Tarzana, CA, USA). Frozen human mobi | Full Paper |
2009 | Lee, CH;Marion, NW;Hollister, S;Mao, JJ; | Tissue formation and vascularization in anatomically shaped human joint condyle ectopically in vivo | Tissue engineering. Part A | 19563263 | hMSCs were isolated from fresh bone marrow samples of multiple anonymous adult donors (AllCells, Berkeley, CA) per our prior methods. Purification of hMSCs was accomplished by Save | Full Paper |
2009 | 李文芳, ;黄元庆, ; | 基于虚拟仪器和 DDE 发电机组温度实时数据采集系统的研制 | 厦门大学学报 (自然科学版) | 其对应的 DDE 服务名,主题名和项名应用与服务器端一致, 分 别是"wd" ,"DDE wd Test"和"AllCells" .在LabWindows/CVI 实时数据库中, 操作的对象是点AllCells , 是一组油温参数的集合, 可以 Save | Full Paper | |
2009 | Hall, AA;Leonardo, CC;Collier, LA;Rowe, DD;Willing, AE;Pennypacker, KR; | Delayed treatments for stroke influence neuronal death in rat organotypic slice cultures subjected to oxygen glucose deprivation | Neuroscience | 19723565 | HUCBC (AllCells, LLC, Inc.) were thawed and resuspended in DMEM containing DNase (1ug/ml) to prevent aggregation. HUCBC were washed twice and resuspended at a concentration of 108 cells/ml. Cultures were maintained in a standard tissue culture incuba | Full Paper |
2009 | Fukushima-Shintani, M;Suzuki, K;Iwatsuki, Y;Abe, M;Sugasawa, K;Hirayama, F;Kawasaki, T;Nakahata, T; | AKR-501 (YM477) a novel orally-active thrombopoietin receptor agonist | European journal of haematology | 19183407 | Human cord blood (CB) CD34+ cells (AllCells, Berkeley, CA, USA) at a density of 2500 cells/0.75 mL were suspended in MegaCult™-C (StemCell Technologies Inc., Vancouver, Canada) containing either AKR-501 dissolved in DMSO or rhTPO, and plated in two-w | Full Paper |
2009 | Zhao, Z;He, X;Bi, Y;Xia, Y;Tao, N;Li, L;Ma, Q; | Induction of CYP4F3 by benzene metabolites in human white blood cells in vivo in human promyelocytic leukemic cell lines and ex vivo in human blood neutrophils | Drug metabolism and disposition: the biological fate of chemicals | 19029204 | Whole blood from a healthy male adult was purchased from AllCells, LLC (Emeryville, CA). Neutrophils were isolated according a protocol provided by AllCells, LLC. In brief, whole Save | Full Paper |
2009 | Nishino, T;Miyaji, K;Ishiwata, N;Arai, K;Yui, M;Asai, Y;Nakauchi, H;Iwama, A; | Ex vivo expansion of human hematopoietic stem cells by a small-molecule agonist of c-MPL | Experimental hematology | 19744539 | 0.5 U/mL rhEPO, and 10 ng/mL rhTPO, respectively. Human cord blood (hCB) CD34+ cells were purchased from Lonza (Basel, Switzerland) and AllCells (Berkeley, CA, USA) or purified from hCB. Fresh hCB cells were obtained from the Tokyo Cord Blood Bank (T | Full Paper |
2009 | Giammona, LM;Panuganti, S;Kemper, JM;Apostolidis, PA;Lindsey, S;Papoutsakis, ET;Miller, WM; | Mechanistic studies on the effects of nicotinamide on megakaryocytic polyploidization and the roles of NAD+ levels and SIRT inhibition | Experimental hematology | 19715739 | Cultures were initiated in T-flasks with previously frozen mPB CD34-selected cells (AllCells; Berkeley, CA or Fred Hutchinson Cancer Research Center; Seattle, WA) and maintained at a concentration of 100,000 to 300,000 cells/ml as described [8]. Most | Full Paper |
2009 | Gibson, JD;Jakuba, CM;Boucher, N;Holbrook, KA;Carter, MG;Nelson, CE; | Single-cell transcript analysis of human embryonic stem cells | Integrative biology : quantitative biosciences from nano to macro | 20023769 | We demonstrate the qualitative and quantitative power of single-cell transcript analysis to characterize transcriptome dynamics in human embryonic stem cells (hESC's). Single-cell Save | Full Paper |
2009 | Wells, R;Guth, A;Lappin, M;Dow, S; | Anti-endothelial cell antibodies in dogs with immune-mediated hemolytic anemia and other diseases associated with high risk of thromboembolism | Journal of veterinary internal medicine | 19192144 | AllCells LLC, Emeryville, CA bSigma Aldrich, St Louis, MO cGibco-InVitrogen, San Diego, CA dHyclone, Logan, UT eJackson ImmunoResearch, West Grove, PA fDako-Cytomation, Fort Collins, CO | Full Paper |
2009 | Harriman, WD;Collarini, EJ;Cromer, RG;Dutta, A;Strandh, M;Zhang, F;Kauvar, LM; | Multiplexed Elispot assay | Journal of immunological methods | 19084532 | Normal human PBMCs were obtained from AllCells (Emeryville, CA). For CellSpot assays, 105 PBMC, Th1 or Th2 selected cells were suspended in activation medium with phorbol myristate acetate (PMA) at 50 ng/mL and Ionomycin at 1 µg/mL or control medium, | Full Paper |
2009 | Sugiyama, E;Lee, SJ;Lee, SS;Kim, WY;Kim, SR;Tohkin, M;Hasegawa, R;Okuda, H;Kawamoto, M;Kamatani, N;Sawada, J;Kaniwa, N;Saito, Y;Shin, JG; | Ethnic differences of two non-synonymous single nucleotide polymorphisms in CDA gene | Drug metabolism and pharmacokinetics | 20045991 | DNA from 200 healthy Chinese-Americans was extracted from cord blood samples purchased from AllCells (Emeryville, CA, USA). Peripheral blood samples from healthy Caucasian- Save | Full Paper |
2009 | Ye, D;Garcia-Manero, G;Kantarjian, HM;Xiao, L;Vadhan-Raj, S;Fernandez, MH;Nguyen, MH;Medeiros, LJ;Bueso-Ramos, CE; | Analysis of Aurora kinase A expression in CD34(+) blast cells isolated from patients with myelodysplastic syndromes and acute myeloid leukemia | Journal of hematopathology | 19669217 | reverse transcription efficiency. Relative RNA level was reported via standard delta delta Ct (δδ Ct). cDNA purified from normal CD34+ hematopoietic progenitor cells was used as calibrator sample (Allcells, LLC, Emeryville, CA, USA). Immunocytochemi | Full Paper |
2009 | Nagel, I; | Identifizierung von pathogenetisch relevanten Genen bei lymphatischen Neoplasien unter besonderer Berücksichtigung des langen Arms von Chromosom 14 | Thesis | Probanden isoliert. Zur Normalisierung der quantitativen Reverse Transkription (RT)-PCR-Analyse des TRAF3-Gens wurde „Normal Peripheral Blood B Cells cDNA (CD19+)“ der Firma Allcells (Emeryville, CA, USA) verwendet. Für die quantitativen RT-PCR-Analy | Full Paper | |
2009 | Mahadevan, D;Choi, J;Cooke, L;Simons, B;Riley, C;Klinkhammer, T;Sud, R;Maddipoti, S;Hehn, S;Garewal, H;Spier, C; | Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3 and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival and Migration | Human genomics and proteomics : HGP | 20981323 | CD19+ normal PB B-cell RNA from 4 normal volunteers was purchased from AllCells, CA. Control RNA was obtained from normal peripheral (PB) B-cells from 4 volunteers (AllCells, CA) Save | Full Paper |
2009 | Garzon, R; | MicroRNA profiling of megakaryocytes | Methods in molecular biology (Clifton, N.J.) | 18839118 | It is critical to use high-quality CD34+ stem cells. These cells could be obtained commercially from Allcells.com or Stemcell technologies. Other cheaper alternatives include the use of cord blood-selected CD34+ cells. Failure to use high-quality pro | Full Paper |
2009 | Huynh, H;Kaba, M;Rudra, S;Zheng, J;Wu, C;Lodish, H;Zhang, C; | IGFBP2 Supports ex vivo Expansion of Hematopoietic Stem Cells | Research and Perspectives in Endocrine Interactions | Fresh and cryopreserved human cord blood cells were purchased from Cambrex, StemCell Technologies Inc., and AllCells. All of the cells were from pooled donors. CD34 + or CD133 Save | Full Paper | |
2009 | Harirchian, P; | Validation of EBI2 as a Novel Therapeutic Target for Autoimmune Disorders | Thesis | Fresh leukapheresed blood (300 mL) from healthy donors was purchased from Allcells (Emeryville, CA). On the same day of leukapheresis, peripheral blood mononuclear cells (PBMC) Save | Full Paper | |
2009 | Bachstetter, AD; | The role of neuroinflammation in regulating the age-related decline in neurogenesis | Thesis | Cell preparation: Cryopreserved human umbilical cord blood mononuclear cells (UCBMCs) were obtained from Saneron CCEL Therapeutics, Inc. (Tampa, FL, USA). Cryopreserved Human peripheral blood cells (PBMC) (mononuclear fraction) were obtained from All | Full Paper | |
2009 | Carter, CC; | Infection of hematopoietic progenitor cells by HIV-1 | Thesis | Cell culture. Fresh whole bone marrow aspirates were obtained commercially (AllCells Ltd.). Umbilical cord blood was obtained following scheduled cesarean section procedures, in accordance with an IRB-approved protocol. Bone marrow mononuclear cells | Full Paper | |
2009 | O'Brien, JJ; | Mechanisms of megakaryopoiesis and platelet production: Implications for inflammation and disease | Thesis | Human CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5x10" cells per well in a 12-well plate and cultured in serum-free medium as previously described (Zeuner et al. 2007) and supplemented with 100 ng/mL o | Full Paper | |
2009 | Hall, AA; | Immunomodulatory effects of novel therapies for stroke | Thesis | DMEM without glucose (Invitrogen, Carlsbad, CA) for OGD. HUCBC (AllCells) were thawed and resuspended in DMEM containing DNase (1ug/ml) to prevent aggregation. HUCBC were washed twice and resuspended at a concentration of 108 cells/ml. HUCBC (106 ) | Full Paper | |
2009 | Schwengberg, S; | Use of embryonic stem cell derived cardiomyocytes for determination of cardiac cytotoxicity | Journal of Pharmacological and Toxicological Methods | Hescheler for providing Patch Clamp analysis; Leonard Fink for development and programming of the automated analysis software; our colleagues at Axiogenesis AG and ReachBio Save | Full Paper | |
2008 | Ramalingam, S;Forster, J;Naret, C;Evans, T;Sulecki, M;Lu, H;Teegarden, P;Weber, MR;Belani, CP; | Dual inhibition of the epidermal growth factor receptor with cetuximab, an IgG1 monoclonal antibody, and gefitinib, a tyrosine kinase inhibitor, in patients with refractory non-small cell lung cancer (NSCLC): a phase I study | J Thorac Oncol | 18317068 | 38. FFPE tumor samples were submitted to QualTek (Newtown, PA) for EGFR expression analysis by immunohistochemistry. The intensity of staining was scored as: 3 = high, 2 = moderate, 1 = low/minimal, and 0 = no staining. RESULTS. Patient Demographics | Full Paper |
2008 | Jiang, P;Enomoto, A;Jijiwa, M;Kato, T;Hasegawa, T;Ishida, M;Sato, T;Asai, N;Murakumo, Y;Takahashi, M; | An actin-binding protein Girdin regulates the motility of breast cancer cells | Cancer Res. | 18316593 | Tissue arrays of human malignant tumors and matched normal adjacent tissues were purchased from Folio Biosciences, Inc. Immunohistochemisty was performed using standard techniques. Antigen retrieval was performed with microwave treatment in a 0.01 mo | Full Paper |
2008 | Lu, Y;Wang, J;Xu, Y;Koch, AE;Cai, Z;Chen, X;Galson, DL;Taichman, RS;Zhang, J; | CXCL16 functions as a novel chemotactic factor for prostate cancer cells in vitro | Mol. Cancer Res. | 18344492 | High-density tissue microarrays were constructed by Folio Biosciences with clinical samples obtained from a cohort of 80 patients. Tumors were graded using the Gleason grading system and examined to identify areas of benign prostate, prostate cancer, | Full Paper |
2008 | Wang, J;Shiozawa, Y;Wang, J;Wang, Y;Jung, Y;Pienta, KJ;Mehra, R;Loberg, R;Taichman, RS; | The role of CXCR7/RDC1 as a chemokine receptor for CXCL12/SDF-1 in prostate cancer | J. Biol. Chem. | 18057003 | tumor size, or clinical outcome. Breast cancer arrays were purchased from Folio Biosciences (Columbus, OH) (ARY-HH0058), which were generated from clinical samples derived from a cohort of 80 patients. In this case an | Full Paper |
2008 | Gallagher, J;Lynch, FW;Barragry, J; | A prolactinoma masked by a herbal remedy | Eur. J. Obstet. Gynecol. Reprod. Biol. | 17298863 | Full Paper | |
2008 | Meszaros, R;Strate, I;Pera, EM;Durbeej, M; | Expression of the novel gene Ened during mouse and Xenopus embryonic development | Int. J. Dev. Biol. | 18956345 | Mul- tiple alignment of the amino acid sequences was carried out with MultAlin software (http://bioinfo.genopole-toulouse.prd.fr/multalin/multalin.html). Whole-mount and radioactive in situ hybridization (Phylogeny, Inc. Columbus | Full Paper |
2008 | Kawada, M;Inoue, H;Arakawa, M;Ikeda, D; | Transforming growth factor-β1 modulates tumor-stromal cell interactions of prostate cancer through insulin-like growth factor-I | Anticancer research | Immunohistochemistry. Tissue arrays of human prostate cancer were purchased from Folio Biosciences (Columbus, OH, USA). The tissue sections were deparaffinized in xylene and rehydrated in descending alcohols into water | Full Paper | |
2008 | Cherry, RA;Nichols, PA;Snavely, TM;David, MT;Lynch, FC; | Prophylactic inferior vena cava filters: do they make a difference in trauma patients? | J Trauma | 18784566 | Full Paper | |
2008 | Yang, L;Anderson, DE;Baecher-Allan, C;Hastings, WD;Bettelli, E;Oukka, M;Kuchroo, VK;Hafler, DA; | IL-21 and TGF-beta are required for differentiation of human T(H)17 cells | Nature | 18469800 | PBMCs were obtained from the peripheral blood of healthy subjects or from cord blood (AllCells) in compliance with institutional review board (IRB) protocols. CD4 + T cells were Save | Full Paper |
2008 | Cisse, B;Caton, ML;Lehner, M;Maeda, T;Scheu, S;Locksley, R;Holmberg, D;Zweier, C;den Hollander, NS;Kant, SG;Holter, W;Rauch, A;Zhuang, Y;Reizis, B; | Transcription factor E2-2 is an essential and specific regulator of plasmacytoid dendritic cell development | Cell | 18854153 | Total RNA from purified human peripheral blood cell types (Allcells) or sorted murine cells was reverse transcribed and assayed by SYBR Green-based real-time PCR with MX3000P Save | Full Paper |
2008 | Paoluzzi, L;Gonen, M;Bhagat, G;Furman, RR;Gardner, JR;Scotto, L;Gueorguiev, VD;Heaney, ML;Manova, K;O'Connor, OA; | The BH3-only mimetic ABT-737 synergizes the antineoplastic activity of proteasome inhibitors in lymphoid malignancies | Blood | 18591385 | Mononuclear cells from peripheral blood samples (PBMCs) of healthy donors were purchased from Allcells (Emeryville, CA). All cell lines were grown as previously described. Save | Full Paper |
2008 | Luqman, M;Klabunde, S;Lin, K;Georgakis, GV;Cherukuri, A;Holash, J;Goldbeck, C;Xu, X;Kadel, EE;Lee, SH;Aukerman, SL;Jallal, B;Aziz, N;Weng, WK;Wierda, W;O'Brien, S;Younes, A; | The antileukemia activity of a human anti-CD40 antagonist antibody, HCD122, on human chronic lymphocytic leukemia cells | Blood | 18497318 | B-cell chronic lymphocytic leukemia (B-CLL) is a lymphoproliferative disorder characterized by the surface expression of CD20, CD5 antigens, as well as the receptor CD40. Activation Save | Full Paper |
2008 | Zhang, CC;Kaba, M;Iizuka, S;Huynh, H;Lodish, HF; | Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantation | Blood | 18202223 | Fresh and cryopreserved human cord blood cells were purchased from Cambrex (East Rutherford, NJ), StemCell Technologies, and AllCells (Berkeley, CA). All of cells were from pooled donors. CD34+ or CD133+ purity checked by flow cytometry was higher th | Full Paper |
2008 | Garzon, R;Volinia, S;Liu, CG;Fernandez-Cymering, C;Palumbo, T;Pichiorri, F;Fabbri, M;Coombes, K;Alder, H;Nakamura, T;Flomenberg, N;Marcucci, G;Calin, GA;Kornblau, SM;Kantarjian, H;Bloomfield, CD;Andreeff, M;Croce, CM; | MicroRNA signatures associated with cytogenetics and prognosis in acute myeloid leukemia | Blood | 18187662 | from 4 healthy donors were purchased from Allcells (Emeryville, CA). Bone marrow CD34 + progenitors from 10 healthy donors were purchased from Allcells. In vitro differentiated Save | Full Paper |
2008 | Mercher, T;Cornejo, MG;Sears, C;Kindler, T;Moore, SA;Maillard, I;Pear, WS;Aster, JC;Gilliland, DG; | Notch signaling specifies megakaryocyte development from hematopoietic stem cells | Cell stem cell | 18786418 | using a FACSAria (BD Biosciences). Human CD34+ bone marrow cells were obtained from AllCells. For in vitro cocultures of LSK cells with stroma, analyses were gated on forward/side scatter profile and CD45+ or CD45+GFP+ cells. Apoptosis assays were pe | Full Paper |
2008 | Tuller, T;Kupiec, M;Ruppin, E; | Evolutionary rate and gene expression across different brain regions | Genome biology | 18811952 | names and other properties appears in Additional data file 12. The human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA, USA), Clontech (Palo Alto, CA, USA), AllCells (Berkeley, CA, USA), Clonetics/BioWhittak | Full Paper |
2008 | Song, W;van der Vliet, HJ;Tai, YT;Prabhala, R;Wang, R;Podar, K;Catley, L;Shammas, MA;Anderson, KC;Balk, SP;Exley, MA;Munshi, NC; | Generation of antitumor invariant natural killer T cell lines in multiple myeloma and promotion of their functions via lenalidomide: a strategy for immunotherapy | Clinical cancer research : an official journal of the American Association for Cancer Research | 18980990 | Normal bone marrow samples were purchased from AllCells. Multiple myeloma patient blood and bone marrow samples were obtained from Dana-Farber Cancer Institute following Save | Full Paper |
2008 | Lu, J;Guo, S;Ebert, BL;Zhang, H;Peng, X;Bosco, J;Pretz, J;Schlanger, R;Wang, JY;Mak, RH;Dombkowski, DM;Preffer, FI;Scadden, DT;Golub, TR; | MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte progenitors | Developmental cell | 18539114 | Human umbilical cord blood was harvested at Brigham and Women’s Hospital with informed patient consent under an IRB approved protocol. Adult human bone marrow cells were obtained from AllCells, LLC. Mononuclear cells were purified by Ficoll Hypaque s | Full Paper |
2008 | Solomon, DA;Kim, JS;Cronin, JC;Sibenaller, Z;Ryken, T;Rosenberg, SA;Ressom, H;Jean, W;Bigner, D;Yan, H;Samuels, Y;Waldman, T; | Mutational inactivation of PTPRD in glioblastoma multiforme and malignant melanoma | Cancer research | 19074898 | Normal human astrocytes (NHA) were obtained from Clonetics and AllCells. All cell lines were grown in DMEM + 10% fetal bovine serum (FBS) at 37C in 5% CO 2 . Save | Full Paper |
2008 | Lau, YK;Murray, LB;Houshmandi, SS;Xu, Y;Gutmann, DH;Yu, Q; | Merlin is a potent inhibitor of glioma growth | Cancer research | 18632626 | Normal human astrocytes (NHA cells) were obtained from ALLCELLS, Inc. Cells were maintained following the providers' and manufacturers' instructions. Anti-v5 epitope (Invitrogen), Save | Full Paper |
2008 | Solomon, DA;Kim, JS;Jenkins, S;Ressom, H;Huang, M;Coppa, N;Mabanta, L;Bigner, D;Yan, H;Jean, W;Waldman, T; | Identification of p18 INK4c as a tumor suppressor gene in glioblastoma multiforme | Cancer research | 18381405 | Normal human astrocytes (NHAs) were obtained from Clonetics and Allcells. All cell lines were grown in DMEM + 10% fetal bovine serum at 37 in 5% CO 2 . Save | Full Paper |
2008 | Bonini, MG;Stadler, K;Silva, SO;Corbett, J;Dore, M;Petranka, J;Fernandes, DC;Tanaka, LY;Duma, D;Laurindo, FR;Mason, RP; | Constitutive nitric oxide synthase activation is a significant route for nitroglycerin-mediated vasodilation | Proceedings of the National Academy of Sciences of the United States of America | 18562300 | eNOS (Ser-1177 and Thr-495) were from Santa Cruz and used according to the manufacturer's instructions. HUVECs were from Allcells and cultured as described (24). All other chemicals used were analytical grade or superior | Full Paper |
2008 | O'Hare, T;Eide, CA;Tyner, JW;Corbin, AS;Wong, MJ;Buchanan, S;Holme, K;Jessen, KA;Tang, C;Lewis, HA;Romero, RD;Burley, SK;Deininger, MW; | SGX393 inhibits the CML mutant Bcr-AblT315I and preempts in vitro resistance when combined with nilotinib or dasatinib | Proceedings of the National Academy of Sciences of the United States of America | 18367669 | Hematopoietic Colony Forming Assays of Normal Bone Marrow. Bone marrow mononuclear cells (AllCells) from three normal individuals were cultured with SGX393. Cells (5 × 104) were plated in methylcellulose containing 50 ng/ml SCF, 10 ng/ml GM-CSF, and | Full Paper |
2008 | Lund, TC;Tolar, J;Orchard, PJ; | Granulocyte colony-stimulating factor mobilized CFU-F can be found in the peripheral blood but have limited expansion potential | Haematologica | 18403392 | Briefly, blood from G-CSF treated healthy volunteers was purchased from AllCells, LLC (Emeryville, CA, USA) (see Online Supplementary Appendix). The use of all tissue was approved by the Committee on the Use of Human Subjects in Research at the Unive | Full Paper |
2008 | Van Scott, MR;Mertsching, E;Negrou, E;Miles, J;Stallings, HW;Graff, C;Kehry, MR; | Systemic administration of an Fcgamma-Fc(epsilon)-fusion protein in house dust mite sensitive nonhuman primates | Clinical immunology (Orlando, Fla.) | 18583194 | Human basophils were differentiated from CD34+ cord blood progenitors (AllCells, LLC, Emeryville, CA) by 4- to 5-weeks of culture in complete medium [RPMI 1640 (ATCC, Manassas, VA), 10% FBS (HyClone, Logan , UT), 10 μg/ml gentamicin (Sigma-Aldrich, S | Full Paper |
2008 | Reyna, SM;Ghosh, S;Tantiwong, P;Meka, CS;Eagan, P;Jenkinson, CP;Cersosimo, E;Defronzo, RA;Coletta, DK;Sriwijitkamol, A;Musi, N; | Elevated toll-like receptor 4 expression and signaling in muscle from insulin-resistant subjects | Diabetes | 18633101 | e performed Western analysis and real-time PCR of EMR1, a marker for monocyte/macrophage infiltration, in muscle tissue of insulin-resistant subjects and in cultured myotubes. Unlike CD14+ monocytes (AllCells, Emeryville, CA), which expressed EMR1, n | Full Paper |
2008 | Smith, LM;Nesterova, A;Ryan, MC;Duniho, S;Jonas, M;Anderson, M;Zabinski, RF;Sutherland, MK;Gerber, HP;Van Orden, KL;Moore, PA;Ruben, SM;Carter, PJ; | CD133/prominin-1 is a potential therapeutic target for antibody-drug conjugates in hepatocellular and gastric cancers | British journal of cancer | 18542072 | idoma AC133. 1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and normal human primary cells (HREC, hepatocytes) were obtained from Cambrex (Lonza, Switzerland) and AllCells (Emeryville, CA, USA), respectively. Cell | Full Paper |
2008 | Xu, Y;Theobald, V;Sung, C;DePalma, K;Atwater, L;Seiger, K;Perricone, MA;Richards, SM; | Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients | Journal of translational medicine | 18945350 | oulter Immunomics, San Diego, CA). Frozen PBMC Samples: Frozen peripheral blood mononuclear cells (PBMCs), screened HIV negative, were used in this study. PBMC from blood of HLA-A2 healthy subjects (AllCells, LLC, Emeryville, CA and American Red Cro | Full Paper |
2008 | DiBlasio-Smith, EA;Arai, M;Quinet, EM;Evans, MJ;Kornaga, T;Basso, MD;Chen, L;Feingold, I;Halpern, AR;Liu, QY;Nambi, P;Savio, D;Wang, S;Mounts, WM;Isler, JA;Slager, AM;Burczynski, ME;Dorner, AJ;LaVallie, ER; | Discovery and implementation of transcriptional biomarkers of synthetic LXR agonists in peripheral blood cells | Journal of translational medicine | 18925943 | Fresh human PBMC, T cells, B cells, and monocytes from normal human donors were purchased from AllCells (Emeryville, CA). Each cell set was derived from the same donor for Save | Full Paper |
2008 | Inamdar, KV;O'Brien, S;Sen, S;Keating, M;Nguyen, MH;Wang, X;Fernandez, M;Thomazy, V;Medeiros, LJ;Bueso-Ramos, CE; | Aurora-A kinase nuclear expression in chronic lymphocytic leukemia | Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc | 18931650 | fam-ATCAAGATCATTGCTCCTCCTGAGCGC-bhq) at 100 nM concentrations. Real-time quantitative RT-PCR was performed in an automatic MX3000P real-time RT-PCR thermal cycler (Stratagene, La Jolla, CA, USA). Relative RNA level was reported via standard delta del | Full Paper |
2008 | Peterson, SJ;Drummond, G;Kim, DH;Li, M;Kruger, AL;Ikehara, S;Abraham, NG; | L-4F treatment reduces adiposity, increases adiponectin levels, and improves insulin sensitivity in obese mice | Journal of lipid research | 18426778 | Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in α-MEM (Invitrogen) supplemented with 10% heat-inactivated FBS (Invitrogen), and 1% antibiotic/antimycotic solutio | Full Paper |
2008 | Liebler, JM;Lutzko, C;Banfalvi, A;Senadheera, D;Aghamohammadi, N;Crandall, ED;Borok, Z; | Retention of human bone marrow-derived cells in murine lungs following bleomycin-induced lung injury | American journal of physiology. Lung cellular and molecular physiology | 18515407 | Human BMDC preparation and administration. Whole adult human bone marrow cells (Cambrex, Walkersville, MD or AllCells, Emeryville, CA) were used as the source of BMDC under a protocol approved by the Institutional Review Board of the University of So | Full Paper |
2008 | Mina-Osorio, P;Winnicka, B;O'Conor, C;Grant, CL;Vogel, LK;Rodriguez-Pinto, D;Holmes, KV;Ortega, E;Shapiro, LH; | CD13 is a novel mediator of monocytic/endothelial cell adhesion | Journal of leukocyte biology | 18495788 | 37 cells, WEHI‐274.1 (murine myeloid cell line), C33A cells, and hemangioendothelioma (EOMA) cells [19] were from the American Type Cell Culture Collection (ATCC; Manassas, VA, USA). PBMC were from AllCells (Emeryville, CA, USA). Anti‐CD13 mAb 452 an | Full Paper |
2008 | Kopetzki, E;Jekle, A;Ji, C;Rao, E;Zhang, J;Fischer, S;Cammack, N;Sankuratri, S;Heilek, G; | Closing two doors of viral entry: intramolecular combination of a coreceptor- and fusion inhibitor of HIV-1 | Virology journal | 18452606 | s were obtained from the NIH AIDS Research and Reference Reagent program. Human embryonic kidney cells 293-T and HEK293-EBNA cells were obtained from ATCC, Manassas, VA. Human PBMC were obtained from AllCells (Emeryville, CA), stimulated for one day | Full Paper |
2008 | Tomchuck, SL;Zwezdaryk, KJ;Coffelt, SB;Waterman, RS;Danka, ES;Scandurro, AB; | Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responses | Stem cells (Dayton, Ohio) | 17916800 | Human MSCs were obtained from our collaborators at the Tulane University Center for Gene Therapy led by Darwin J. Prockop, M.D., Ph.D. Additionally, MSCs were obtained from two commercial suppliers Cambrex BioScience (Walkersville, MD) and Allcells ( | Full Paper |
2008 | Lee, EJ;Baek, M;Gusev, Y;Brackett, DJ;Nuovo, GJ;Schmittgen, TD; | Systematic evaluation of microRNA processing patterns in tissues, cell lines, and tumors | RNA (New York, N.Y.) | 18025253 | Human cancer cell lines were obtained from ATCC or other investigators. Human total RNA from 20 tissues was from Ambion. CD19+ human B cells were purchased from AllCells. RNA from pancreas and liver tumor and adjacent benign pancreas were obtained fr | Full Paper |
2008 | Clark, PA;Moioli, EK;Sumner, DR;Mao, JJ; | Porous implants as drug delivery vehicles to augment host tissue integration | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 18198217 | Fresh bone marrow samples of multiple adult male donors (AllCells, Berkeley, CA, USA) were used to isolate MSC, per our previous methods (19, 23). Nonadherent cells were removed Save | Full Paper |
2008 | Mittal, R;Prasadarao, NV; | Outer membrane protein A expression in Escherichia coli K1 is required to prevent the maturation of myeloid dendritic cells and the induction of IL-10 and TGF-beta | Journal of immunology (Baltimore, Md. : 1950) | 18684958 | Dendritic cells (DCs) are professional APCs that direct both cellular and humoral immune responses. Escherichia coli K1 causes meningitis in neonates; however, the interactions Save | Full Paper |
2008 | Fukaya, Y;Shimada, H;Wang, LC;Zandi, E;DeClerck, YA; | Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma | The Journal of biological chemistry | 18450743 | transferrin (0.25 μg/ml), selenium (0.25 ng/ml), and l-glutamine. Human BMSC were obtained from ALLCELLS (Emeryville, CA) and maintained in basal medium with stimulatory supplements containing basic fibroblast growth factor (10 ng/ml) | Full Paper |
2008 | Tong, Q;Hirschler-Laszkiewicz, I;Zhang, W;Conrad, K;Neagley, DW;Barber, DL;Cheung, JY;Miller, BA; | TRPC3 is the erythropoietin-regulated calcium channel in human erythroid cells | The Journal of biological chemistry | 18276585 | Human erythroid progenitors/precursors were cultured using a two-phase liquid culture system (31, 32). Cells harvested at day 8 of Phase II were predominantly proerythroblasts and basophilic normoblasts. CD34+ cells were purchased from AllCells, LLC | Full Paper |
2008 | Wang, M;Windgassen, D;Papoutsakis, ET; | Comparative analysis of transcriptional profiling of CD3+, CD4+ and CD8+ T cells identifies novel immune response players in T-cell activation | BMC genomics | 18485203 | ng regulation of T-cell activation in the natural CD3+ population, as well as in the CD4+ and CD8+ subsets. Methods Cells and culture system Healthy-donor peripheral blood mononuclear cells (PBMCs) (AllCells, Berkeley, CA) were used to negatively-se | Full Paper |
2008 | Moioli, EK;Clark, PA;Sumner, DR;Mao, JJ; | Autologous stem cell regeneration in craniosynostosis | Bone | 18023269 | Human mesenchymal stem cells isolated from bone marrow (AllCells, Berkeley, CA) were cultured in 6-well plates (3 × 10 4 cells/well) with osteogenic supplements as described above Save | Full Paper |
2008 | Liang, W;Rahaman, M;Day, D;Marion, N;Riley, G;Mao, J; | Bioactive borate glass scaffold for bone tissue engineering | Journal of Non-Crystalline Solids | Bone marrow derived human mesenchymal stem cells, hbMSCs (AllCells; Berkeley, CA) were cultured for 18 days in either DMEM complete (89% DMEM 10% FBS, 1% penicillin - Save | Full Paper | |
2008 | Sikorska, M;Sandhu, JK;Deb-Rinker, P;Jezierski, A;Leblanc, J;Charlebois, C;Ribecco-Lutkiewicz, M;Bani-Yaghoub, M;Walker, PR; | Epigenetic modifications of SOX2 enhancers, SRR1 and SRR2, correlate with in vitro neural differentiation | Journal of neuroscience research | 18293417 | Primary human astrocytes (HA; prepared from fetuses between 9 and 10 week of gestation) were purchased from AllCells LLC. (Emeryville, CA). The cells were cultured in poly-L-lysine-coated T25-cm2 flasks using basal medium containing stimulatory suppl | Full Paper |
2008 | Papaliodis, D;Boucher, W;Kempuraj, D;Michaelian, M;Wolfberg, A;House, M;Theoharides, TC; | Niacin-induced "flush" involves release of prostaglandin D2 from mast cells and serotonin from platelets: evidence from human cells in vitro and an animal model | The Journal of pharmacology and experimental therapeutics | 18784348 | Freshly isolated human platelets were purchased from AllCells, LLC (Emeryville, CA) and sent to us overnight. Platelets were used the day of arrival after washing as indicated below. Save | Full Paper |
2008 | Kim, DH;Burgess, AP;Li, M;Tsenovoy, PL;Addabbo, F;McClung, JA;Puri, N;Abraham, NG; | Heme oxygenase-mediated increases in adiponectin decrease fat content and inflammatory cytokines tumor necrosis factor-alpha and interleukin-6 in Zucker rats and reduce adipogenesis in human mesenchymal stem cells | The Journal of pharmacology and experimental therapeutics | 18334666 | Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential medium ( Save | Full Paper |
2008 | Fuhrken, PG;Chen, C;Apostolidis, PA;Wang, M;Miller, WM;Papoutsakis, ET; | Gene Ontology-driven transcriptional analysis of CD34+ cell-initiated megakaryocytic cultures identifies new transcriptional regulators of megakaryopoiesis | Physiological genomics | 18252802 | Mk and G cultures were initiated with fresh (AllCells; Berkeley, CA) or frozen (Hematopoietic Cell Processing Core, Fred Hutchinson Cancer Center, Seattle, WA) granulocyte colony-stimulating factor (G-CSF)-mobilized human peripheral blood CD34+ cells | Full Paper |
2008 | Stirewalt, DL;Meshinchi, S;Kopecky, KJ;Fan, W;Pogosova-Agadjanyan, EL;Engel, JH;Cronk, MR;Dorcy, KS;McQuary, AR;Hockenbery, D;Wood, B;Heimfeld, S;Radich, JP; | Identification of genes with abnormal expression changes in acute myeloid leukemia | Genes, chromosomes & cancer | 17910043 | Cancer Research Center (FHCRC) or purchased from commercially available vendors (Cambrex, Rutherford, NJ or AllCells, Emeryville, CA). BM (N = 7) and PB (N = 19) samples from 26 adult AML patients were obtained from the FHCRC's Leukemia Repository. T | Full Paper |
2008 | Li, YY;Bao, M;Meurer, J;Skuballa, W;Bauman, JG;Doecke, WD;Zollner, TM; | The identification of a small molecule inhibitor that specifically reduces T cell-mediated adaptive but not LPS-mediated innate immunity by T cell membrane-monocyte contact bioassay | Immunology letters | 18241931 | PBMCs were either purchased (Allcells, Emeryville, CA) or isolated from healthy donor blood after informed consent. Berlex environmental health & safety (EHS) policies and Save | Full Paper |
2008 | Ramsubir, S;Nonaka, T;Girbés, CB;Carpentier, S;Levade, T;Medin, JA; | In vivo delivery of human acid ceramidase via cord blood transplantation and direct injection of lentivirus as novel treatment approaches for Farber disease | Molecular genetics and metabolism | 18805722 | CD34+ cells were obtained from AllCells, LLC (Berkeley, CA). Cells were pre-stimulated for 12 h in StemSpan SFEM medium (Stem Cell Technologies, Vancouver, BC, Canada) supplemented with recombinant human SCF, Flt3-L, thrombopoietin (all at 50 ng/ml), | Full Paper |
2008 | Huang, D;Pereboev, AV;Korokhov, N;He, R;Larocque, L;Gravel, C;Jaentschke, B;Tocchi, M;Casley, WL;Lemieux, M;Curiel, DT;Chen, W;Li, X; | Significant alterations of biodistribution and immune responses in Balb/c mice administered with adenovirus targeted to CD40(+) cells | Gene therapy | 18046426 | using Adeno-X Rapid Titer kit according to the manufacturer's instructions (BD Biosciences, San Jose, CA, USA). DC culture, Ad infection and luciferase activity assay Myeloid DCs were obtained from AllCELLS, Emeryville, CA, USA. Over 80% of these mo | Full Paper |
2008 | Oh, W;Kim, DS;Yang, YS;Lee, JK; | Immunological properties of umbilical cord blood-derived mesenchymal stromal cells | Cellular immunology | 18495100 | To assess T-cell reactivity against allogeneic cell populations, human responder PBMCs (1 × 105/well) were cocultured with inactivated allogeneic PBMCs (1 × 105/well) or hUCB-MSCs (1 × 102, 1 × 103 or 1 × 104/well) in 96-well tissue culture plates. T | Full Paper |
2008 | Seo, BM;Sonoyama, W;Yamaza, T;Coppe, C;Kikuiri, T;Akiyama, K;Lee, JS;Shi, S; | SHED repair critical-size calvarial defects in mice | Oral diseases | 18938268 | Human bone marrow cells were purchased from commercially available resources (AllCells LLC, Berkeley, CA, USA). To identify putative bone marrow mesenchymal stem cells, single Save | Full Paper |
2008 | Moioli, EK;Clark, PA;Chen, M;Dennis, JE;Erickson, HP;Gerson, SL;Mao, JJ; | Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues | PloS one | 19081793 | Bone marrow cells were isolated from whole marrow aspirates of the iliac crest of each of three healthy male donors (AllCells, Berkeley, CA) and plated as in Fig. 1A. Human Save | Full Paper |
2008 | Yamaza, T;Miura, Y;Bi, Y;Liu, Y;Akiyama, K;Sonoyama, W;Patel, V;Gutkind, S;Young, M;Gronthos, S;Le, A;Wang, CY;Chen, W;Shi, S; | Pharmacologic stem cell based intervention as a new approach to osteoporosis treatment in rodents | PloS one | 18612428 | amethasone (Sigma-Aldrich Co.) were added into the culture for 6 weeks. Human BMMSC culture Human bone marrow aspirates from healthy human adult volunteers (20-35 years of age) were purchased from AllCells LLC and cultured as reported previously [21 | Full Paper |
2008 | Ghosh, A;Park, JY;Fenno, C;Kapila, YL; | Porphyromonas gingivalis, gamma interferon, and a proapoptotic fibronectin matrix form a synergistic trio that induces c-Jun N-terminal kinase 1-mediated nitric oxide generation and cell death | Infection and immunity | 18838522 | Human primary normal peripheral blood neutrophils (AllCells, CA) were maintained in Iscove's modified Dulbecco's medium containing 5% fetal bovine serum, 2 mM glutamine, 25 mM Save | Full Paper |
2008 | Hanten, JA;Vasilakos, JP;Riter, CL;Neys, L;Lipson, KE;Alkan, SS;Birmachu, W; | Comparison of human B cell activation by TLR7 and TLR9 agonists | BMC immunology | 18652679 | 3') was obtained from (Genosys, Woodlands, TX). Isolation and treatment of human peripheral blood B cells Human peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers from AllCells, LLC. (Berkeley, CA) and Memorial Blood Ce | Full Paper |
2008 | López, MC;Lawrence, DA; | Proficiency testing experience for viable CD34+ stem cell analysis | Transfusion | 18298596 | Human peripheral blood was collected in blood collection tubes (Cyto-Chex BCT, Streck Laboratories, La Vista, NE), which contain a preservative reagent for flow cytometry. Frozen CD34+ stem cells from cord blood (AllCells LLC, Berkeley, CA) were thaw | Full Paper |
2008 | Tang, H;Lu, JY;Zheng, X;Yang, Y;Reagan, JD; | The psoriasis drug monomethylfumarate is a potent nicotinic acid receptor agonist | Biochemical and biophysical research communications | 18722346 | 2B were purchased from AllCells (Emeryville, CA) and were of greater than 95% purity. Human CD14+ monocytes and NHEK were from Cambrex (East Rutherford, NJ) and cultured as Save | Full Paper |
2008 | Bachstetter, AD;Pabon, MM;Cole, MJ;Hudson, CE;Sanberg, PR;Willing, AE;Bickford, PC;Gemma, C; | Peripheral injection of human umbilical cord blood stimulates neurogenesis in the aged rat brain | BMC neuroscience | 18275610 | cord blood mononuclear cells (UCBMCs) were obtained from Saneron CCEL Therapeutics, Inc. (Tampa, FL, USA). Cryopreserved Human peripheral blood cells (PBMC) (mononuclear fraction) were obtained from AllCells, LLC (Emeryville, CA, USA). Just prior to | Full Paper |
2008 | Mashiyama, ST;Hansen, CM;Roitman, E;Sarmiento, S;Leklem, JE;Shultz, TD;Ames, BN; | An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiency | Analytical biochemistry | 17963712 | , respectively, in measuring DNA-uracil in all the samples used in experiments described in this paper (lymphocytes from the B6 deficiency study and lymphocytes from AllCells). Uracil Save | Full Paper |
2008 | Park, EJ;Kim, ES;Weber, HP;Wright, RF;Mooney, DJ; | Improved bone healing by angiogenic factor-enriched platelet-rich plasma and its synergistic enhancement by bone morphogenetic protein-2 | The International journal of oral & maxillofacial implants | 19014150 | Fresh human adult whole blood was purchased from AllCells (Emeryville, CA, USA). Peripheral blood mononuclear cells (PBMNCs) and platelets were separately isolated by a density gradient centrifugation (Histopaque-1077, Sigma, St. Louis, MO, USA) acco | Full Paper |
2008 | Thamm, DH;O'Brien, MG;Vail, DM; | Serum vascular endothelial growth factor concentrations and postsurgical outcome in dogs with osteosarcoma | Veterinary and comparative oncology | 19178672 | Vascular endothelial growth factor (VEGF) is a key angiogenic growth factor, playing putative roles in both tumour growth and metastasis. The purpose of this study was to correlate Save | Full Paper |
2008 | Antonescu, CR;Wu, K;Xing, GL;Cao, M;Turpaz, Y;Leversha, MA;Hubbell, E;Maki, RG;Miyada, CG;Pillai, R; | DNA copy number analysis in gastrointestinal stromal tumors using gene expression microarrays | Cancer informatics | 19259404 | from the American Type Culture Collection (ATCC, Manassas, VA). DNA was extracted from the cultured cells using the DNA Maxi Kit (Qiagen, Inc., Valencia, CA). DNA from normal blood was obtained from AllCells, LLC (Emeryville, CA). GIST sample DNA was | Full Paper |
2008 | Li, M;Rossi, JJ; | Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cells | Methods in molecular biology (Clifton, N.J.) | 18679631 | Target cells: K562 cells, a chronic myelogenous leukemia cell line (ATCC, #CCL-243). CD34+ hematopoietic stem cells, purchased from AllCells (Berkley, CA, USA) or purified from umbilical cord blood or bone marrow by anti-CD34 antibody-coupled magneti | Full Paper |
2008 | Campbell-Anson, RE;Kentor, D;Wang, YJ;Bushnell, KM;Li, Y;Vence, LM;Radvanyi, LG; | A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technology | Journal of immune based therapies and vaccines | 18321390 | Sources of PBMC for DC generation PBMC were obtained from peripheral blood leukopheresis products obtained from non-mobilized normal donors (LifeBlood, Memphis, TN), or G-CSF-mobilized normal donors (AllCells, Berkeley, CA). Products were collected i | Full Paper |
2008 | Tiainen, J; | Structured equity products and portfolio compositions of financially advised investors | Thesis | RESULTS I will show in this thesis that behavioural biases of the retail investors are associated to proportions of portfolio invested in structured capital guaranteed products and in equity Save | Full Paper | |
2008 | Shi, M; | The Role of Janus-Kinase-3 in CD4+ T Cell Proliferation and Differentiation: A Dissertation | Thesis | Aliquots of cryopreserved peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryville, CA). PBMC were resuspended in complete RPMI 1640 medium (RPMI 1640 medium supplemented with 10% fetal bovine serum, penicillin/streptomycin, | Full Paper | |
2008 | Ziegler, LA; | Targeting lentivector gene delivery to hematopoietic stem and progenitor cells in vivo | Thesis | Fresh, unfractionated human PBMCs (2 x 106 ) (AllCells) were incubated with concentrated virus with total TUs of 10 x 106 (based on the titer on 293T/CD20 cells). LPS (50 μg/ml) was then added for B cells to survive and grow. After 2 days, cells wer | Full Paper | |
2008 | Wang, M; | Genomic-scale transcriptional analysis of T-cell activation reveals novel genes and signaling programs | Thesis | Healthy-donor peripheral blood mononuclear cells (PBMCs) (AllCells, Berkeley, CA) were used to negatively-select CD3+ T cells, CD4+ and CD8+ T cells (Pan T Cell Isolation Kit II, CD4+ T Cell Isolation Kit II, and CD8+ T Cell Isolation Kit II, Milteny | Full Paper | |
2008 | Bedi, R; | Characterization of the activities of the human C/EBPϵ activator and repressor isoforms in myelopoiesis | Thesis | 25 ml/cm2 . After preloading and before prestimulation, the plates were washed with PBS to remove any inhibitory molecules in the viral supernatant. Cord blood CD34+ cells purchased from AllCells (CA, USA) were thawed according to the supplier's inst | Full Paper | |
2007 | Aziz, MH;Manoharan, HT;Church, DR;Dreckschmidt, NE;Zhong, W;Oberley, TD;Wilding, G;Verma, AK; | Protein kinase Cepsilon interacts with signal transducers and activators of transcription 3 (Stat3), phosphorylates Stat3Ser727, and regulates its constitutive activation in prostate cancer | Cancer Res. | 17875724 | Prostate cancer specimens were obtained at radical prostatectomy. The University of Wisconsin Human Subjects Committee approved the use of these human prostate specimens. Tissue microarrays were obtained from Folio Biosciences.4 Tissue microarrays we | Full Paper |
2007 | Senzer, N;Nemunaitis, J;Nemunaitis, M;Lamont, J;Gore, M;Gabra, H;Eeles, R;Sodha, N;Lynch, FJ;Zumstein, LA;Menander, KB;Sobol, RE;Chada, S; | p53 therapy in a patient with Li-Fraumeni syndrome | Mol. Cancer Ther. | 17483435 | Full Paper | |
2007 | Gagna, CE;Clark Lambert, W; | Cell biology, chemogenomics and chemoproteomics - application to drug discovery | Expert Opin Drug Discov | 23484648 | TMAs have been expensive and limited in terms of commercial availability [8,56-59]. Only recently have they surfaced commercially on a large scale (e.g., Cytomyx, Folio Biosciences, Chemicon). Many different types of high quality TMAs now exist, such | Full Paper |
2007 | Flores, J;Cepeda, IL;Cornfeldt, ML;O'Kusky, JR;Doudet, DJ; | Characterization and survival of long-term implants of human retinal pigment epithelial cells attached to gelatin microcarriers in a model of Parkinson disease | J. Neuropathol. Exp. Neurol. | 17620984 | All primary antibodies were previously assessed for hRPE cell specificity in human RPE cultured monolayer and RPE-choroid eye tissue, and for nonspecificity to striatal rat tissue (Dr. S. Bernstein, QualTek Molecular Laboratories, Santa Barbara, CA, | Full Paper |
2007 | Lynch, FC; | Use of a covered stent for percutaneous transrenal occlusion of the ureter | J Vasc Interv Radiol | 18004001 | Full Paper | |
2007 | Cantwell, CP;Lynch, FC; | Caudal migration of a G2 filter during carbon dioxide cavography | J Vasc Interv Radiol | 17538152 | Full Paper | |
2007 | Lee, S;Sha, Q;Wu, X;Calenda, G;Peng, J; | Expression profiles of mouse Kell, XK, and XPLAC mRNA | J. Histochem. Cytochem. | 17189525 | For the ISH experiments, the custom service from Phylogeny Inc. (Columbus, OH) was used (Lyons et al. 1990; Patapoutian et al. 1993). Briefly, tissue sections were hybridized overnight at 55C in 50% deionized formamide, 0.3 M NaCl, 20 mM Tris-HCl, pH | Full Paper |
2007 | Tanimoto, R;Abarzua, F;Sakaguchi, M;Takaishi, M;Nasu, Y;Kumon, H;Huh, NH; | REIC/Dkk-3 as a potential gene therapeutic agent against human testicular cancer | Int. J. Mol. Med. | 17273781 | Tissues, cells and reagents. A testicular carcinoma tissue microarray was purchased from Folio Biosciences (Columbus, OH). Surgically resected testicular cancer tissues were fixed with 4% paraformaldehyde and tissue sections were made under conventio | Full Paper |
2007 | Calin, GA;Liu, CG;Ferracin, M;Hyslop, T;Spizzo, R;Sevignani, C;Fabbri, M;Cimmino, A;Lee, EJ;Wojcik, SE;Shimizu, M;Tili, E;Rossi, S;Taccioli, C;Pichiorri, F;Liu, X;Zupo, S;Herlea, V;Gramantieri, L;Lanza, G;Alder, H;Rassenti, L;Volinia, S;Schmittgen, TD;Kipps, TJ;Negrini, M;Croce, CM; | Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomas | Cancer cell | 17785203 | An additional set of 3 normal CD19+/CD5+ positive B cells, not used for microarray studies, was purchased from AllCells (Berkeley, CA), and used as an independent confirmation set. In all instances the qRT-PCR data confirmed the microarray data. RNA | Full Paper |
2007 | Wang, L;Wang, J;Blaser, BW;Duchemin, AM;Kusewitt, DF;Liu, T;Caligiuri, MA;Briesewitz, R; | Pharmacologic inhibition of CDK4/6: mechanistic evidence for selective activity or acquired resistance in acute myeloid leukemia | Blood | 17537993 | Entry into the cell cycle is mediated by cyclin-dependent kinase 4/6 (CDK4/6) activation, followed by CDK2 activation. We found that pharmacologic inhibition of the Flt3 internal tandem Save | Full Paper |
2007 | Mulligan, G;Mitsiades, C;Bryant, B;Zhan, F;Chng, WJ;Roels, S;Koenig, E;Fergus, A;Huang, Y;Richardson, P;Trepicchio, WL;Broyl, A;Sonneveld, P;Shaughnessy, JD;Bergsagel, PL;Schenkein, D;Esseltine, DL;Boral, A;Anderson, KC; | Gene expression profiling and correlation with outcome in clinical trials of the proteasome inhibitor bortezomib | Blood | 17185464 | The aims of this study were to assess the feasibility of prospective pharmacogenomics research in multicenter international clinical trials of bortezomib in multiple myeloma and to Save | Full Paper |
2007 | Brogdon, JL;Xu, Y;Szabo, SJ;An, S;Buxton, F;Cohen, D;Huang, Q; | Histone deacetylase activities are required for innate immune cell control of Th1 but not Th2 effector cell function | Blood | 17008546 | as described previously.22,23 Human neutrophils were obtained from AllCells (Berkeley, CA). Mouse bone marrow-derived DCs were prepared as described previously.24 Murine Th1 and Th2 effector cells were differentiated from DO11.10 mice as described | Full Paper |
2007 | Mahadevan, D;Klinkhammer, T;Cooke, L;Riley, C;Choi, J;Sinari, S;Miller, T;Spier, C; | CLL Transcript and Serum Profiling Identifies Distinct Oncogenic Pathways in Rai Stage Progression. | Blood | Full Paper | ||
2007 | Robinson, S;Simmons, P;Brouard, N;Kidd, S;Yang, H;Decker, W;Xing, D;Steiner, D;Ng, J;Thomas, M;Champlin, R;de Lima, M;Hosing, C;Cooper, L;Singh, H;Zweidler-McKay, P;Shpall, E; | Efficacy of ‘Off-the-Shelf’, Commercially-Available, Third-Party Mesenchymal Stem Cells (MSC) in Ex Vivo Cord Blood (CB) Co-Culture Expansion. | Blood | INTRODUCTION: Our previous studies have shown that clinically-relevant levels of hematopoietic stem and progenitor cell (HSPC) expansion are possible by ex vivo co-culture of cord blood (CB) mononuclear cells (MNC) with third-party bone marrow (BM)-d | Full Paper | |
2007 | Majeti, R;Park, CY;Weissman, IL; | Identification of a hierarchy of multipotent hematopoietic progenitors in human cord blood | Cell stem cell | 18371405 | Normal human bone marrow mononuclear cells were purchased from AllCells Inc. (Emeryville, CA). Human cord blood was collected from placentas and/or umbilical cords obtained Save | Full Paper |
2007 | Scholl, C;Bansal, D;Döhner, K;Eiwen, K;Huntly, BJ;Lee, BH;Rücker, FG;Schlenk, RF;Bullinger, L;Döhner, H;Gilliland, DG;Fröhling, S; | The homeobox gene CDX2 is aberrantly expressed in most cases of acute myeloid leukemia and promotes leukemogenesis | The Journal of clinical investigation | 17347684 | and all patients and volunteers gave informed consent according to the Declaration of Helsinki. Seven of the 10 normal BMMC samples and the 3 normal BM samples that were used for isolating HSCs, CMPs, GMPs, and MEPs were purchased from AllCells | Full Paper |
2007 | Xin, X;Hussain, M;Mao, JJ; | Continuing differentiation of human mesenchymal stem cells and induced chondrogenic and osteogenic lineages in electrospun PLGA nanofiber scaffold | Biomaterials | 17010425 | Bone-marrow-derived hMSCs were purchased from AllCells (Berkeley, CA, USA). The donor was a 25 year-old healthy male volunteer without additional identifier. In our previous work, we have isolated hMSCs from multiple donors [5]. In this report, the i | Full Paper |
2007 | Zou, H;Harrington, JJ;Sugumar, A;Klatt, KK;Smyrk, TC;Ahlquist, DA; | Detection of colorectal disease by stool defensin assay: an exploratory study | Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association | 17531545 | These cDNA samples and a purchased neutrophil cNDA (AllCells, Emeryville, CA) were amplified by real-time polymerase chain reaction (PCR) in an iCycler (Bio-Rad, Hercules, CA). Save | Full Paper |
2007 | Zhao, Y;Parkhurst, MR;Zheng, Z;Cohen, CJ;Riley, JP;Gattinoni, L;Restifo, NP;Rosenberg, SA;Morgan, RA; | Extrathymic generation of tumor-specific T cells from genetically engineered human hematopoietic stem cells via Notch signaling | Cancer research | 17363559 | CD133 + progenitor cells were enriched from mononuclear cells from UCB (Allcells, Berkeley, CA) by depleting CD3 + cells using Dynabeads human CD3 (Invitrogen, Carlsbad, CA) Save | Full Paper |
2007 | Verhelle, D;Corral, LG;Wong, K;Mueller, JH;Moutouh-de Parseval, L;Jensen-Pergakes, K;Schafer, PH;Chen, R;Glezer, E;Ferguson, GD;Lopez-Girona, A;Muller, GW;Brady, HA;Chan, KW; | Lenalidomide and CC-4047 inhibit the proliferation of malignant B cells while expanding normal CD34+ progenitor cells | Cancer research | 17234786 | Cell culture. Namalwa CSN.70 and LP-1 cells were purchased from DSMZ (Brauschweig, Germany), U266 was purchased from the American Type Culture Collection (Manassas, VA), and cord blood CD34+ cells were purchased from AllCells (Emeryville, CA). Namalw | Full Paper |
2007 | Pariente, N;Morizono, K;Virk, MS;Petrigliano, FA;Reiter, RE;Lieberman, JR;Chen, IS; | A novel dual-targeted lentiviral vector leads to specific transduction of prostate cancer bone metastases in vivo after systemic administration | Molecular therapy : the journal of the American Society of Gene Therapy | 17653099 | endothelial cells were obtained from AllCells (Emeryville, CA) and grown in human umbilical vein endothelial cell basal medium plus stimulatory supplement (AllCells, Emelyville, CA). Save | Full Paper |
2007 | Sonoyama, W;Seo, BM;Yamaza, T;Shi, S; | Human Hertwig's epithelial root sheath cells play crucial roles in cementum formation | Journal of dental research | 17586703 | Human bone marrow mesenchymal stem cells (BMMSCs) were purchased from a commercially available resource (AllCells LLC, Berkeley, CA, USA), and cultured as previously described (Batouli et al., 2003). Cemento/osteogenic differentiation was induced and | Full Paper |
2007 | Li, L;Piloto, O;Kim, KT;Ye, Z;Nguyen, HB;Yu, X;Levis, M;Cheng, L;Small, D; | FLT3/ITD expression increases expansion, survival and entry into cell cycle of human haematopoietic stem/progenitor cells | British journal of haematology | 17359372 | Activating mutation of FLT3 by internal tandem duplications (ITDs) in the juxtamembrane region is the most common molecular aberration found in acute myeloid leukaemia (AML). In Save | Full Paper |
2007 | Kikuchi, J;Shimizu, R;Wada, T;Ando, H;Nakamura, M;Ozawa, K;Furukawa, Y; | E2F-6 suppresses growth-associated apoptosis of human hematopoietic progenitor cells by counteracting proapoptotic activity of E2F-1 | Stem cells (Dayton, Ohio) | 17600109 | magnetic cell sorting; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com) from human bone marrow mononuclear cells (BM-MNCs) (purchased from AllCells, Berkeley, CA, http://www.allcells.com) or cord blood mononuclear cells (CB | Full Paper |
2007 | Telford, WG;Bradford, J;Godfrey, W;Robey, RW;Bates, SE; | Side population analysis using a violet-excited cell-permeable DNA binding dye | Stem cells (Dayton, Ohio) | 17185610 | (FBS)/10% dimethyl sulfoxide in liquid nitrogen until use. Frozen human cord blood was obtained from AllCells LLC (Emeryville, CA, http://www.allcells.com) and thawed immediately before use. A549 lung carcinoma cells were grown in Dulbecco's modified | Full Paper |
2007 | Fang, D;Seo, BM;Liu, Y;Sonoyama, W;Yamaza, T;Zhang, C;Wang, S;Shi, S; | Transplantation of mesenchymal stem cells is an optimal approach for plastic surgery | Stem cells (Dayton, Ohio) | 17170063 | Human bone marrow cells were purchased from commercially available resources (AllCells LLC, Berkeley, CA,http://www.allcells.com/). To identify putative bone marrow MSCs, single-cell suspension of 1 × 106 of bone marrow mononuclear cells were seeded | Full Paper |
2007 | Stosich, MS;Mao, JJ; | Adipose tissue engineering from human adult stem cells: clinical implications in plastic and reconstructive surgery | Plastic and reconstructive surgery | 17255658 | Human mesenchymal stem cells were isolated from fresh bone marrow samples of an anonymous adult male donor (AllCells, Berkeley, Calif.) by following our previous methods. After Save | Full Paper |
2007 | Kim, JS;Lee, C;Bonifant, CL;Ressom, H;Waldman, T; | Activation of p53-dependent growth suppression in human cells by mutations in PTEN or PIK3CA | Molecular and cellular biology | 17060456 | were described in a previous study (27). BJ-hTERT and RPE-hTERT cells were obtained from Clontech (Mountain View, CA). Normal human astrocytes (NHA) were obtained from AllCells LLC (Emeryville, CA) and cultured as recommended. HEK293, MCF10A, and A17 | Full Paper |
2007 | Chen, C;Fuhrken, PG;Huang, LT;Apostolidis, P;Wang, M;Paredes, CJ;Miller, WM;Papoutsakis, ET; | A systems-biology analysis of isogenic megakaryocytic and granulocytic cultures identifies new molecular components of megakaryocytic apoptosis | BMC genomics | 17953764 | tudies of individual candidate genes. Methods Hematopoietic cultures Mk and G cell cultures were initiated with fresh or frozen G-CSF-mobilized human peripheral blood CD34+ cells from normal donors (AllCells; Berkeley, CA) and cultured as described | Full Paper |
2007 | Vose, SC;Holland, NT;Eskenazi, B;Casida, JE; | Lysophosphatidylcholine hydrolases of human erythrocytes, lymphocytes, and brain: sensitive targets of conserved specificity for organophosphorus delayed neurotoxicants | Toxicology and applied pharmacology | 17663017 | Whole human blood from seven subjects and lymphocytes (isolated by aphaeresis using a leukopac filter) from three subjects were obtained from AllCells LLC (Emeryville, CA). Mouse Save | Full Paper |
2007 | Zeng, Z;Yuan, X;Liu, G;Zeng, X;Zeng, X;Ng, H;Chen, H;Jiang, T;Akasaki, Y;Kessey, K;Black, KL;Yu, JS; | Manipulation of proliferation and differentiation of human bone marrow-derived neural stem cells in vitro and in vivo | Journal of neuroscience research | 17131390 | Human bone marrow cells (HBMC) were isolated from fresh human whole bone marrow (AllCells, Berkely, CA) by gradient density centrifugation (Lee et al., 2003). HBMC were plated at a density of 2 × 106 cells/ml/well onto 24-well plates (Corning, Cornin | Full Paper |
2007 | Huang, LT;Paredes, CJ;Papoutsakis, ET;Miller, WM; | Gene expression analysis illuminates the transcriptional programs underlying the functional activity of ex vivo-expanded granulocytes | Physiological genomics | 17550995 | of PB granulocytes should reflect their full activation potential, because RNA levels would not be expected to change very much during cell transportation and processing by AllCells ( | Full Paper |
2007 | Uno, S;Kinoshita, Y;Azuma, Y;Tsunenari, T;Yoshimura, Y;Iida, S;Kikuchi, Y;Yamada-Okabe, H;Fukushima, N; | Antitumor activity of a monoclonal antibody against CD47 in xenograft models of human leukemia | Oncology reports | 17390064 | The ligation of CD47 induces the apoptosis of leukemic cells in a caspase-independent manner. We generated a monoclonal antibody against CD47 (mAb-MABL) that possibly induced Save | Full Paper |
2007 | Jenkins, J;Calzone, KA; | Establishing the essential nursing competencies for genetics and genomics | Journal of nursing scholarship : an official publication of Sigma Theta Tau International Honor Society of Nursing | 17393960 | 129This annotation involves adding the units and labels to allcells containing no references to other cells. The systemthen analyzes the cells with formulas containing referencesand Save | Full Paper |
2007 | Furuhata, S;Ando, K;Oki, M;Aoki, K;Ohnishi, S;Aoyagi, K;Sasaki, H;Sakamoto, H;Yoshida, T;Ohnami, S; | Gene expression profiles of endothelial progenitor cells by oligonucleotide microarray analysis | Molecular and cellular biochemistry | 17203242 | Among the many tissue stem or progenitor cells recently being unveiled, endothelial progenitor cells (EPCs) have attracted particular attention, not only because of their cardinal role in Save | Full Paper |
2007 | Yourek, G;Hussain, MA;Mao, JJ; | Cytoskeletal changes of mesenchymal stem cells during differentiation | ASAIO journal (American Society for Artificial Internal Organs : 1992) | 17413564 | Human mesenchymal stem cells were isolated from commercially available whole bone marrow (AllCells, Berkeley, CA) using RosetteSep (StemCell Technologies, Vancouver, BC) according to the manufacturer’s instructions. The RosetteSep antibody cocktail c | Full Paper |
2007 | Baysal, BE; | A recurrent stop-codon mutation in succinate dehydrogenase subunit B gene in normal peripheral blood and childhood T-cell acute leukemia | PloS one | 17487275 | 80°C. cDNAs synthesized from column-purified normal PBMC cell-type subsets, including monocytes (CD14+), NK cells (CD56+, negative selection), B (CD19+) and T lymphocytes (CD3+), were purchased from AllCells, LLC Inc. through StemCell Technologies. E | Full Paper |
2007 | Stosich, MS;Bastian, B;Marion, NW;Clark, PA;Reilly, G;Mao, JJ; | Vascularized adipose tissue grafts from human mesenchymal stem cells with bioactive cues and microchannel conduits | Tissue engineering | 17824832 | Human MSCs were isolated from fresh bone marrow samples of two anonymous adult donors (AllCells, Berkeley, CA), per our previous methods.After transferring bone marrow sample Save | Full Paper |
2007 | Popov, BV;Serikov, VB;Petrov, NS;Izusova, TV;Gupta, N;Matthay, MA; | Lung epithelial cells induce endodermal differentiation in mouse mesenchymal bone marrow stem cells by paracrine mechanism | Tissue engineering | 17630877 | Human hematopoietic stem cells (CD34þ) were purchased from AllCells Co. (Berkeley, CA) and confirmed to be 96% pure using FACScan. Cells were washed twice in PBS, labeled with PKH26 (Sigma) according to manufacturer’s instructions, and mixed with epi | Full Paper |
2007 | Moioli, EK;Hong, L;Mao, JJ; | Inhibition of osteogenic differentiation of human mesenchymal stem cells | Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society | 17537129 | hMSCs were isolated from donated human bone marrow samples (AllCells, Berkeley, CA) and plated in monolayers in six-well plates at a density of 3×104 cells/well per our previous methods36 (Figure 1A and B). The isolated hMSCs were cultured-expanded a | Full Paper |
2007 | Anderson, RM;Stevens, DL;Sumption, ND;Townsend, KM;Goodhead, DT;Hill, MA; | Effect of linear energy transfer (LET) on the complexity of alpha-particle-induced chromosome aberrations in human CD34+ cells | Radiation research | 17474795 | Frozen human bone marrow CD34 + cells were obtained commercially (Poietics, Cambrex and AllCells, LLC) in vials of 1 × 10 6 cells (samples are anonymized and exempt from Save | Full Paper |
2007 | Sawyer, L;Hanson, D;Castro, G;Luckett, W;Dubensky, TW;Stassinopoulos, A; | Inactivation of parvovirus B19 in human platelet concentrates by treatment with amotosalen and ultraviolet A illumination | Transfusion | 17524098 | CD34+ cells isolated from granulocyte-colony-stimulating factor-mobilized human peripheral blood were obtained from AllCells (Berkeley, CA) and stimulated to proliferate and Save | Full Paper |
2007 | Wang, TT;Tio, M;Lee, W;Beerheide, W;Udolph, G; | Neural differentiation of mesenchymal-like stem cells from cord blood is mediated by PKA | Biochemical and biophysical research communications | 17466951 | Cell culture and growth profiling. MNCs (AllCells) were seeded in MyeloCult medium (Stem Cell Technologies) supplemented with 1 × 10−7 M dexamethasone (Sigma). Adherent cells were trypsinized (0.125% Trypsin-EDTA) and passaged at near-confluency (70- | Full Paper |
2007 | Chen, L;Gao, Z;Zhu, J;Rodgers, GP; | Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrow | Experimental hematology | 17588473 | Human bone marrow CD133+ cells (AllCells, Berkeley, CA, USA) were cultured for 14-28 days in the presence of EPO or G-CSF. All cells were grown in media that included stem cell factor (SCF, 50 ng/mL), interleukin-3 (IL-3, 10 ng/mL), granulocyte-macro | Full Paper |
2007 | Chua, KN;Chai, C;Lee, PC;Ramakrishna, S;Leong, KW;Mao, HQ; | Functional nanofiber scaffolds with different spacers modulate adhesion and expansion of cryopreserved umbilical cord blood hematopoietic stem/progenitor cells | Experimental hematology | 17577926 | Frozen human umbilical cord blood CD34+ HSPCs were purchased from AllCells, LLC (Emeryville, CA, USA). The CD34+ purity in the post-thawed HSPC was determined to be 98% by flow cytometry and the viability was determined to be more than 97% by Trypan | Full Paper |
2007 | Zwezdaryk, KJ;Coffelt, SB;Figueroa, YG;Liu, J;Phinney, DG;LaMarca, HL;Florez, L;Morris, CB;Hoyle, GW;Scandurro, AB; | Erythropoietin, a hypoxia-regulated factor, elicits a pro-angiogenic program in human mesenchymal stem cells | Experimental hematology | 17379074 | Cambrex BioScience (Walkersville, MD, USA) and Allcells (Emeryville, CA, USA), to ensure enough variability of the starting cell population and make certain that findings are universal and not unique to single donor pools derived from a unique source | Full Paper |
2007 | Cheng, X;Macvittie, T;Meisenberg, B;Welty, E;Farese, A;Tadaki, D;Takebe, N; | Human brain endothelial cells (HUBEC) promote SCID repopulating cell expansion through direct contact | Growth factors (Chur, Switzerland) | 18049950 | The objective of this study was to re-evaluate the previously published hematopoietic stem cell (HSC) expansion work using human brain endothelial cells (HUBEC). The expansion Save | Full Paper |
2007 | Basiji, DA;Ortyn, WE;Liang, L;Venkatachalam, V;Morrissey, P; | Cellular image analysis and imaging by flow cytometry | Clinics in laboratory medicine | 17658411 | To investigate the utility of imaging flow cytometry for clinical FISH analysis, human peripheral blood mononuclear cells (PBMC) were obtained (AllCells, San Diego, CA) and probed using a FISH in suspension (FISHIS®) protocol developed at Amnis Corpo | Full Paper |
2007 | King, JA; | Cell culture systems inspired by the hematopoietic stem cell niche | Thesis | were maintained between 0.5 and 1x106 cells/mL. 3.3.5 Culture of CD34+ selected cells CD34+ selected cells were purchased from AllCells (Berkeley, CA). AllCells obtained informed consent from volunteers before donors provided cells | Full Paper | |
2007 | Bell, A;Meyer-Ebrecht, D;Bocking, A;Aach, T; | HDR-Microscopy of Cell Specimens: Imaging and Image Analysis | 2007 Conference Record of the Forty-First Asilomar Conference on Signals, Systems and Computers | The counterstain affects allcells, ie, independent of the protein which is stained by the immunocytochemical marker. Hence, marker positive cells will be affected by both stains at once. Save | Full Paper | |
2007 | 이연경, ; | Effects of cationic liposomes on in vitro and in vivo transduction mediated by adenoviral vectors | Thesis | According to theresultofGFP expression in HeLa cells(Figure 3A),nearly allcells were transduced in the presence of the 100 MOIof Ad-vectors. Meanwhile, CAR-deficient B16BL6 cells Save | Full Paper | |
2007 | NGIAP, CK; | Bio-functionalization of Electrospun Nanofibre Scaffolds for Cell Culture Applications | Thesis | Frozen human umbilical cord blood CD34+ HSPCs were purchased from AllCells, which were obtained from normal volunteers participating in an Institute Reviewing Board (IRB) approved donor program (AllCells). The CD34+ purity in the HSPC was determine | Full Paper | |
2007 | Giammona, LM; | Nicotinamide enhances primary human megakaryocytic differentiation from hematopoietic stem cells: Phenotypic characterization and mechanism of action | Thesis | Unless otherwise noted, all reagents were obtained from Sigma-Aldrich (St. Louis, MO). Cultures were initiated with previously frozen human mobilized peripheral blood CD34+ cells (AllCells; Berkeley, CA). Cells were cultured in T-flasks and maintaine | Full Paper | |
2007 | Troken, AJ; | Mesenchymal stem cells and derivatives in hydrogels | Thesis | HMSCs were isolated from commercially available healthy human bone marrow (AllCells, LLC; Berkeley, CA) using RosetteSep kit (StemCell Technologies, Inc., Vancouver, BC, Canada). Bone marrow extracts contain heterogeneous cell populations. MSCs repre | Full Paper | |
2006 | Perk, J;Gil-Bazo, I;Chin, Y;de Candia, P;Chen, JJ;Zhao, Y;Chao, S;Cheong, W;Ke, Y;Al-Ahmadie, H;Gerald, WL;Brogi, E;Benezra, R; | Reassessment of id1 protein expression in human mammary, prostate, and bladder cancers using a monospecific rabbit monoclonal anti-id1 antibody | Cancer Res. | 17108123 | Two slides of commercially available (Folio Biosciences, Columbus, OH) TMAs were also analyzed. The two slides included a total of 143 cores, of which 62 samples were common types of breast cancer, and the rest of the samples were benign (slides #ARY | Full Paper |
2006 | Swales, KE;Korbonits, M;Carpenter, R;Walsh, DT;Warner, TD;Bishop-Bailey, D; | The farnesoid X receptor is expressed in breast cancer and regulates apoptosis and aromatase expression | Cancer Res. | 17047076 | Samples of human infiltrating ductal breast carcinoma and near tumor normal samples were obtained from 10 patients after receiving ethical approval and individual patient consent. Protein was extracted using Cytobuster (Novagen, Merck Biosciences, No | Full Paper |
2006 | Evans, EE;Henn, AD;Jonason, A;Paris, MJ;Schiffhauer, LM;Borrello, MA;Smith, ES;Sahasrabudhe, DM;Zauderer, M; | C35 (C17orf37) is a novel tumor biomarker abundantly expressed in breast cancer | Mol. Cancer Ther. | 17121940 | Staining of formalin-fixed, paraffin-embedded breast carcinoma and normal tissue sections was done in part by QualTek Molecular Labs (Santa Barbara, CA). Ten lobular and 50 invasive ductal (grades 1, 2, and 3) carcinomas were selected from the QualTe | Full Paper |
2006 | Roth, JD;Hughes, H;Kendall, E;Baron, AD;Anderson, CM; | Antiobesity effects of the beta-cell hormone amylin in diet-induced obese rats: effects on food intake, body weight, composition, energy expenditure, and gene expression | Endocrinology | 16935845 | To compare central nervous system gene expression across amylin- and pair-fed-treated rats that had exhibited a similar magnitude of weight loss, four animals from each group were selected for ISH, counterbalanced for their final body weight on the d | Full Paper |
2006 | Couse, JF;Yates, MM;Rodriguez, KF;Johnson, JA;Poirier, D;Korach, KS; | The intraovarian actions of estrogen receptor-alpha are necessary to repress the formation of morphological and functional Leydig-like cells in the female gonad | Endocrinology | 16627580 | ... }X56974 [/nuccore/X56974] 1271–1351 GAAACTGCGAATGGCTCATTAA GAATTACCACAGTTATCCAAGTAGGA Open in a separate window [/pmc/articles/PMC1892221/table/T1/?report=objectonly] IN SITU HYBRIDIZATION Phylogeny Inc. (Columbus, OH) conducted all _in | Full Paper |
2006 | Costello, C;Ward, M; | Search, bioprospecting and biodiversity conservation | Journal of Environmental Economics and Management | in a particular way. When executed in bulk, these tests cost between $4000 and $18,000 per species tested (eg Qualtek Molecular Laboratories, Santa Barbara, CA). We use a range for c of $4000-$18,000. • Discount rate (r | Full Paper | |
2006 | Cantwell, CP;Lynch, FC; | Ureterocutaneous fistula and urostomy exclusion with use of a covered wallstent | J Vasc Interv Radiol | 16614159 | Full Paper | |
2006 | Kutty, RK;Chen, S;Samuel, W;Vijayasarathy, C;Duncan, T;Tsai, JY;Fariss, RN;Carper, D;Jaworski, C;Wiggert, B; | Cell density-dependent nuclear/cytoplasmic localization of NORPEG (RAI14) protein | Biochem. Biophys. Res. Commun. | 16729964 | Immunohistochemistry. Immunohistochemistry was performed by QualTek Molecular Laboratories. Briefly, four-micron human placenta sections were deparaffinized, subjected to 20 min of steam heat induced epitope recovery, and then treated with anti-NORPE | Full Paper |
2006 | Cantwell, C;Waybill, P;Lynch, F;Taubman, K; | Inferior vena cava duplication and deep venous thrombosis | Ann Vasc Surg | 16575497 | Full Paper | |
2006 | Huang, LT; | Transcriptional analysis of ex vivo granulocyte (neutrophil) development | Thesis | Cultures were initiated with fresh or frozen G-CSF mobilized peripheral blood CD34+ cells from normal donors (AllCells, Emeryville, CA), at a seeding density of 3 x 104 cells/mL. Cells were cultured in human long-term medium (HLTM) at a pH of 7.1 an | Full Paper | |
2006 | Chen, C;Huang, X;Atakilit, A;Zhu, QS;Corey, SJ;Sheppard, D; | The Integrin alpha9beta1 contributes to granulopoiesis by enhancing granulocyte colony-stimulating factor receptor signaling | Immunity | 17137800 | Murine bone marrow or peripheral blood cells were collected at autopsy and suspended in phosphate-buffered saline (PBS). Human bone marrow cells were obtained from Allcells (Berkeley, CA). Cells were incubated with primary antibody for 30 min at 4°C, | Full Paper |
2006 | Guerzoni, C;Bardini, M;Mariani, SA;Ferrari-Amorotti, G;Neviani, P;Panno, ML;Zhang, Y;Martinez, R;Perrotti, D;Calabretta, B; | Inducible activation of CEBPB, a gene negatively regulated by BCR/ABL, inhibits proliferation and promotes differentiation of BCR/ABL-expressing cells | Blood | 16418324 | CD34+ normal bone marrow (NBM) cells from different healthy donors were purchased from AllCells (LLC, Berkley, CA) and their use was approved by the Ohio State University Institutional Review Board (IRB). Mononuclear hematopoietic cells from bone mar | Full Paper |
2006 | Notari, M;Neviani, P;Santhanam, R;Blaser, BW;Chang, JS;Galietta, A;Willis, AE;Roy, DC;Caligiuri, MA;Marcucci, G;Perrotti, D; | A MAPK/HNRPK pathway controls BCR/ABL oncogenic potential by regulating MYC mRNA translation | Blood | 16293596 | CD34+ normal bone marrow (NBM) cells from healthy donors were from AllCells (Berkeley, CA). Studies performed with human specimens from The Ohio State University Leukemia Tissue Bank (Columbus, OH), the Division of Hematology, Maisonneuve-Rosemont Ho | Full Paper |
2006 | Choi, J;Garewal, H;Riley, C;Cooke, L;Klinkhammer, T;Maddipati, S;Sud, R;Spier, C;Mahadevan, D; | Transcriptosome and Serum Cytokine Profiling of B-CLL Identifies Oncogenic Pathways in Pathogenesis and Survival. | Blood | Full Paper | ||
2006 | Greaney, P;Nahimana, A;Lagopoulos, L;Etter, AL;Aubry, D;Attinger, A;Beltraminelli, N;Huni, B;Bassi, I;Sordat, B;Demotz, S;Dupuis, M;Duchosal, MA; | A Fas agonist induces high levels of apoptosis in haematological malignancies | Leukemia research | 16181674 | CLL 1, 2 and 3, κ+). These cells were frozen in medium containing 10% DMSO within 10 h after harvesting. Haematopoietic progenitor cells from cord blood were obtained frozen from AllCells (San Mateo, CA) | Full Paper |
2006 | Chua, KN;Chai, C;Lee, PC;Tang, YN;Ramakrishna, S;Leong, KW;Mao, HQ; | Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cells | Biomaterials | 16854459 | Interaction between hematopoietic stem/progenitor cells (HSPCs) and their extra cellular matrix components is an integral part of the signaling control for HSPC survival, proliferation Save | Full Paper |
2006 | La Rosée, P;Jia, T;Demehri, S;Härtel, N;de Vries, P;Bonham, L;Hollenback, D;Singer, JW;Melo, JV;Druker, BJ;Deininger, MW; | Antileukemic activity of lysophosphatidic acid acyltransferase-beta inhibitor CT32228 in chronic myelogenous leukemia sensitive and resistant to imatinib | Clinical cancer research : an official journal of the American Association for Cancer Research | 17085669 | without erythropoietin [for culture of granulocyte-macrophage colony-forming unit (CFU-GM)] were done as described previously (26). Normal bone marrow was purchased commercially (AllCells, LLC, Berkeley, CA). Patient samples were collected from patie | Full Paper |
2006 | Yang, L;Bailey, L;Baltimore, D;Wang, P; | Targeting lentiviral vectors to specific cell types in vivo | Proceedings of the National Academy of Sciences of the United States of America | 16864770 | 106) (AllCells) were incubated with concentrated virus with total TUs of 10 106 fresh human PBMCs (AllCells) were transferred by tail vein injection into each mouse. After 6 h, Save | Full Paper |
2006 | Giammona, LM;Fuhrken, PG;Papoutsakis, ET;Miller, WM; | Nicotinamide (vitamin B3) increases the polyploidisation and proplatelet formation of cultured primary human megakaryocytes | British journal of haematology | 17054670 | Unless otherwise noted, all reagents were obtained from Sigma-Aldrich (St Louis, MO, USA). Cultures were initiated with previously frozen human mobilised peripheral blood (mPB) CD34+ cells (AllCells, Berkeley, CA, USA). Cells were cultured in T-flask | Full Paper |
2006 | Wang, HJ;Yu, CL;Kishi, H;Motoki, K;Mao, ZB;Muraguchi, A; | Suppression of experimental osteoarthritis by adenovirus-mediated double gene transfer | Chinese medical journal | 16934183 | Allcells were grown to approxim ately 60% 一 70% confluence at the third passage before adenoviraI vector transduction experim ents started.The cells were washed with sterile saline Save | Full Paper |
2006 | Cabana, R;Frolova, EG;Kapoor, V;Thomas, RA;Krishan, A;Telford, WG; | The minimal instrumentation requirements for Hoechst side population analysis: stem cell analysis on low-cost flow cytometry platforms | Stem cells (Dayton, Ohio) | 16888279 | Institute Laboratory Animal Facility. Mice were euthanized by gradual CO2 exposure according to NIH guidelines. Bone marrow was extracted from femurs and tibias and used with no further fractionation. Frozen human bone marrow and cord blood were obta | Full Paper |
2006 | Yu, X;Alder, JK;Chun, JH;Friedman, AD;Heimfeld, S;Cheng, L;Civin, CI; | HES1 inhibits cycling of hematopoietic progenitor cells via DNA binding | Stem cells (Dayton, Ohio) | 16513761 | Normal human placental/umbilical cord blood (CB) CD34+ cells were purchased from AllCells (Berkeley, CA, http://www.allcells.com). Normal BM and granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood stem cell (PBSC) CD34+ cells wer | Full Paper |
2006 | McIntosh, K;Zvonic, S;Garrett, S;Mitchell, JB;Floyd, ZE;Hammill, L;Kloster, A;Di Halvorsen, Y;Ting, JP;Storms, RW;Goh, B;Kilroy, G;Wu, X;Gimble, JM; | The immunogenicity of human adipose-derived cells: temporal changes in vitro | Stem cells (Dayton, Ohio) | 16410391 | Human bone marrow was purchased from Cambrex (Walkersville, MD, http://www.cambrex.com) or AllCells, LLC (Berkeley, CA). Bone marrow aspirates were collected with heparin and fractionated over a 1.073 g/ml density gradient (Lymphocyte Separation Medi | Full Paper |
2006 | Miura, M;Miura, Y;Padilla-Nash, HM;Molinolo, AA;Fu, B;Patel, V;Seo, BM;Sonoyama, W;Zheng, JJ;Baker, CC;Chen, W;Ried, T;Shi, S; | Accumulated chromosomal instability in murine bone marrow mesenchymal stem cells leads to malignant transformation | Stem cells (Dayton, Ohio) | 16282438 | Human bone marrow aspirates from healthy adult volunteers were purchased from AllCells, LLC (Berkeley, CA, http://www.allcells.com). To identify putative BMMSCs, single-cell suspension of 1 × 106 of bone marrow mononuclear cells was seeded into 15-cm | Full Paper |
2006 | Muramoto, GG;Chen, B;Cui, X;Chao, NJ;Chute, JP; | Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injury | Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation | 16635788 | Cryopreserved human BM CD34+ (Cambrex, Gaithersburg, MD) or CB CD34+ cells (AllCells, Berkeley, CA) were thawed, washed once, and resuspended at 1 × 106/mL in Iscove modified Dulbecco medium (IMDM; Invitrogen) containing 10% FBS and 1% penicillin/str | Full Paper |
2006 | Wang, J;Wu, X;Simonavicius, N;Tian, H;Ling, L; | Medium-chain fatty acids as ligands for orphan G protein-coupled receptor GPR84 | The Journal of biological chemistry | 16966319 | Quantitative Reverse Transcriptase-mediated PCR (Q-RT-PCR) Analysis—Total RNA from human or mouse tissues (Clontech) and human immune cells (AllCells, LLC) were treated with DNase I (Ambion) before reverse transcription. Q-RT-PCR was performed on an | Full Paper |
2006 | Wang, J;Simonavicius, N;Wu, X;Swaminath, G;Reagan, J;Tian, H;Ling, L; | Kynurenic acid as a ligand for orphan G protein-coupled receptor GPR35 | The Journal of biological chemistry | 16754668 | Cytokine Secretion—Human peripheral blood mononuclear cells and CD14 + monocytes were purchased from Allcells. Peripheral blood mononuclear cells were seeded at a density of Save | Full Paper |
2006 | Slukvin, II;Vodyanik, MA;Thomson, JA;Gumenyuk, ME;Choi, KD; | Directed differentiation of human embryonic stem cells into functional dendritic cells through the myeloid pathway | Journal of immunology (Baltimore, Md. : 1950) | 16493050 | Adult PBMC were purchased from AllCells. Monocytes were isolated by adherence to plastic and were cultured for 6 days in RPMI 1640 (Invitrogen Life Technologies) containing 1% FBS (HyClone) in the presence of 100 ng/ml GM-CSF and 100 ng/ml IL-4 with | Full Paper |
2006 | Zhang, W;Hirschler-Laszkiewicz, I;Tong, Q;Conrad, K;Sun, SC;Penn, L;Barber, DL;Stahl, R;Carey, DJ;Cheung, JY;Miller, BA; | TRPM2 is an ion channel that modulates hematopoietic cell death through activation of caspases and PARP cleavage | American journal of physiology. Cell physiology | 16306129 | RNA from CD34 + cells and fresh monocytes was purchased from AllCells (Foster City, CA). cDNA was prepared from RNA with the Superscript first-strand synthesis system (Invitrogen, Save | Full Paper |
2006 | Geddis, AE;Kaushansky, K; | Endomitotic megakaryocytes form a midzone in anaphase but have a deficiency in cleavage furrow formation | Cell cycle (Georgetown, Tex.) | 16552179 | albumin gradient. For human MKs, CD34+ cells were purchased from AllCells (Berkley, CA) and thawed according to manufacturer’s instructions. The cells were then differentiated by culture in StemPro (Invitrogen) containing 100 ng/ml human TPO, 10 ng/m | Full Paper |
2006 | Shen, Y;Chow, J;Wang, Z;Fan, G; | Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cells | Human molecular genetics | 16870691 | Medium was changed everyday for optimal cell growth. Fetal astrocytes were purchased from AllCells LLC., and cultures were expanded as suggested by the vendor. Fetal brain tissue was obtained from the Surgical Pathology Laboratory at UCLA (Dr. Michae | Full Paper |
2006 | Polson, AG;Zheng, B;Elkins, K;Chang, W;Du, C;Dowd, P;Yen, L;Tan, C;Hongo, JA;Koeppen, H;Ebens, A; | Expression pattern of the human FcRH/IRTA receptors in normal tissue and in B-chronic lymphocytic leukemia | International immunology | 16849395 | Human blood samples were collected from healthy individuals through the Genentech research blood program; bone marrow was obtained from AllCells (Berkeley, CA, USA) and Save | Full Paper |
2006 | Zhang, X;Kim, J;Ruthazer, R;McDevitt, MA;Wazer, DE;Paulson, KE;Yee, AS; | The HBP1 transcriptional repressor participates in RAS-induced premature senescence | Molecular and cellular biology | 16966377 | Oncogene-mediated premature senescence has emerged as a potential tumor-suppressive mechanism in early cancer transitions. Previous work shows that RAS and p38 MAPK Save | Full Paper |
2006 | Feng, Q;Chai, C;Jiang, XS;Leong, KW;Mao, HQ; | Expansion of engrafting human hematopoietic stem/progenitor cells in three-dimensional scaffolds with surface-immobilized fibronectin | Journal of biomedical materials research. Part A | 16739181 | Frozen human CB CD34+ cells were purchased from All-Cells (San Mateo, CA), which were obtained from normal volunteers participating in an Institute Reviewing Board (IRB) approved donor program (AllCells). Cells (92% CD34+, 95% viable) were thawed acc | Full Paper |
2006 | Weigel-Kelley, KA;Yoder, MC;Chen, L;Srivastava, A; | Role of integrin cross-regulation in parvovirus B19 targeting | Human gene therapy | 16972759 | Primary human CD71 erythroid progenitor cells were purchased from AllCells (Berkeley, CA) and received in Iscove's modified Dulbecco's medium (IMDM)- 0.5% bovine serum Save | Full Paper |
2006 | Braselmann, S;Taylor, V;Zhao, H;Wang, S;Sylvain, C;Baluom, M;Qu, K;Herlaar, E;Lau, A;Young, C;Wong, BR;Lovell, S;Sun, T;Park, G;Argade, A;Jurcevic, S;Pine, P;Singh, R;Grossbard, EB;Payan, DG;Masuda, ES; | R406, an orally available spleen tyrosine kinase inhibitor blocks fc receptor signaling and reduces immune complex-mediated inflammation | The Journal of pharmacology and experimental therapeutics | 16946104 | Stimulation of Macrophages Derived from Primary Monocytes with IgG or LPS. Human primary macrophages were derived from CD14+ peripheral blood mononuclear cell (AllCells, LLC, Emeryville, CA) according to the protocol specified in the monocyte isolati | Full Paper |
2006 | Xu, H;Othman, SF;Hong, L;Peptan, IA;Magin, RL; | Magnetic resonance microscopy for monitoring osteogenesis in tissue-engineered construct in vitro | Physics in medicine and biology | 16424591 | Mesenchymal stem cells (MSCs) isolated from fresh adult human bone marrow were provided commercially by AllCells (AllCells LLC, Berkeley, CA). Nucleated cells were incubated in Save | Full Paper |
2006 | Troken, A;Marion, N;Mao, J; | HETEROTYPIC ADIPOGENIC PHENOTYPE WHEN EMPLOYING CHONDROGENIC TREATMENT OF HUMAN MESENCHYMAL STEM CELLS | ASAIO Journal | Donated hMSCs (AllCells, LLC) were isolated and expanded using basal media. Upon 90% confluence, the cultures were plated at a density of 24,000 cells/cm 2 in 6-well plates. Three Save | Full Paper | |
2006 | Yamada, H;Kunisato, A;Kawahara, M;Tahimic, CGT;Ren, X;Ueda, H;Nagamune, T;Katoh, M;Inoue, T;Nishikawa, M;Oshimura, M; | Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome | Journal of human genetics | 16333524 | Both EGFP-21ΔqHAC vectors and ScFvg-21ΔqHAC vectors retained in CHO cells were transferred to 1×107 hBM MNCs (AllCells, LLC, Berkeley, CA, USA) or 1×106 hCB CD34+ cells by microcell-mediated chromosome transfer (MMCT) as previously described (Doherty | Full Paper |
2006 | Wang, T;Blatt, LM;Seiwert, SD; | Immunomodulatory activities of IFN-gamma1b in combination with type I IFN: implications for the use of IFN-gamma1b in the treatment of chronic HCV infections | Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research | 16800786 | IL-4 for 2 weeks), and immature pDCs (IFN-producing cells [IPC], defined as CD11C, CD123, and Lin) were purchased from AllCells, LLC (Berkeley, CA). The procedures for their collection were approved by an Institute Review Board and complied with loca | Full Paper |
2006 | Duramad, P;Tager, IB;Leikauf, J;Eskenazi, B;Holland, NT; | Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1 | Journal of applied toxicology : JAT | 16871525 | emale) who were sampled repeatedly, for experiments1-13, or were purchased (n=8) from AllCells (Berkeley,CA) for experiments 14 -19. Specimens were collecteddirectly into vacutainers with heparin and processedwithin 2 | Full Paper |
2006 | Aslan, H;Zilberman, Y;Arbeli, V;Sheyn, D;Matan, Y;Liebergall, M;Li, JZ;Helm, GA;Gazit, D;Gazit, Z; | Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regeneration | Tissue engineering | 16674300 | Human mesenchymal stem cells (hMSCs) were isolated from the bone marrow of an anonymous healthy donor (AllCells, Berkeley, CA), cultureexpanded and seeded in 6-well plates at a density of 30,000 cells/well. For | Full Paper |
2006 | Peptan, IA;Hong, L;Xu, H;Magin, RL; | MR assessment of osteogenic differentiation in tissue-engineered constructs | Tissue engineering | 16674297 | Human bone marrow cells provided commercially(AllCells Limited Liability Company, Berkeley, CA)were plated at a density of 107nucleated cells per 100mm petri dish and incubated in a culture medium com-posed of Dulbecco’s modified | Full Paper |
2006 | Stroke, IL;Cole, AG;Simhadri, S;Brescia, MR;Desai, M;Zhang, JJ;Merritt, JR;Appell, KC;Henderson, I;Webb, ML; | Identification of CXCR3 receptor agonists in combinatorial small-molecule libraries | Biochemical and biophysical research communications | 16930533 | Human peripheral blood mononuclear cells (AllCells, Berkeley, CA) were cultured for 3 days with 10 μg/mL phytohemagglutinin, then for 4-7 days with 100 U/mL interleukin-2 ( Save | Full Paper |
2006 | Sugo, T;Tachimoto, H;Chikatsu, T;Murakami, Y;Kikukawa, Y;Sato, S;Kikuchi, K;Nagi, T;Harada, M;Ogi, K;Ebisawa, M;Mori, M; | Identification of a lysophosphatidylserine receptor on mast cells | Biochemical and biophysical research communications | 16460680 | The total RNAs from human basophils and eosinophils were purchased from AllCells Limited Liability Company (Berkeley, CA). Total RNA was reverse transcribed using Superscript Save | Full Paper |
2006 | Ong, HT;Timm, MM;Greipp, PR;Witzig, TE;Dispenzieri, A;Russell, SJ;Peng, KW; | Oncolytic measles virus targets high CD46 expression on multiple myeloma cells | Experimental hematology | 16728275 | Fresh bone marrow aspirate from a healthy donor was purchased from AllCells (Berkeley, CA, USA). The mononuclear cell fraction was isolated by Ficoll-Hypaque gradient and used in Save | Full Paper |
2006 | Yoshikubo, T;Inoue, T;Noguchi, M;Okabe, H; | Differentiation and maintenance of mast cells from CD34(+) human cord blood cells | Experimental hematology | 16543066 | Frozen human umbilical cord blood CD34+ cells were purchased from AllCells, LLC (Berkeley, CA, USA). The mouse bone marrow-derived stroma cell line MS-5 was kindly provided by K. Itoh [23]. MS-5 cells were maintained in a minimal essential medium α ( | Full Paper |
2006 | Hong, L;Peptan, IA;Xu, H;Magin, RL; | Nondestructive evaluation of osteogenic differentiation in tissue-engineered constructs | Journal of orthopaedic research : official publication of the Orthopaedic Research Society | 16583444 | Healthy fresh human bone marrow was provided commercially (AllCells Limited Liability Company, Emeryville, CA) and plated at a density of 107 nucleated cells per 100 mm petri dish. The cells were incubated in a basic medium of Dulbecco’s Modified Eag | Full Paper |
2006 | Cole, AG;Stroke, IL;Brescia, MR;Simhadri, S;Zhang, JJ;Hussain, Z;Snider, M;Haskell, C;Ribeiro, S;Appell, KC;Henderson, I;Webb, ML; | Identification and initial evaluation of 4-N-aryl-[1,4]diazepane ureas as potent CXCR3 antagonists | Bioorganic & medicinal chemistry letters | 16213722 | 一般社団法人情 処理学会では複写複製および転載複製に係る著作権を学術著作権協会に委託 しています. 当該利用をご希望の方は, 学術著作権協会 が提供している複製利用許諾システ Save | Full Paper |
2006 | Liu, WM;Li, R;Sun, JZ;Wang, J;Tsai, J;Wen, W;Kohlmann, A;Williams, PM; | PQN and DQN: algorithms for expression microarrays | Journal of theoretical biology | 16889801 | Here, we disclose two triplets of experiments using 3 ml fresh bone marrow from a healthy donor obtained from AllCells, LLC (Berkeley, CA). The sample was stored at for 48 h. Then total RNA was purified from the sample with RNeasy mini kit (Qiagen, | Full Paper |
2006 | Doshi, M;Koyanagi, M;Nakahara, M;Saeki, K;Saeki, K;Yuo, A; | Identification of human neutrophils during experimentally induced inflammation in mice with transplanted CD34+ cells from human umbilical cord blood | International journal of hematology | 17050197 | MBR1520-3 x-ray source (Hitachi Medical, Tokyo, Japan). After 24 hours, mice received intravenous transplants of 1.8 × 105 human CB CD34+ cells (AllCells, Berkeley, CA, USA) or vehicle (saline). The purity and viability of the CB CD34+ cells were gre | Full Paper |
2006 | Marion, N;Mao, J; | Mesenchymal Stem Cells and Tissue Engineering | Methods in Enzymology | Human Bone Marrow-Derived MSCs • Ficoll‐Paque—room temperature (e.g., StemCells, Inc., Vancouver, BC, Canada). • Bone marrow sample—room temperature (e.g., AllCells, LLC, Berkeley, CA). • 10 ml marrow + 5 ml DPBS + 125 U/ml heparin (total volume, 1 | Full Paper | |
2006 | Hawley, R;Ramezani, A;Hawley, T; | Hematopoietic Stem Cells | Adult Stem Cells | peripheral blood cells after informed consent in conformity with a human subjects protocol approved by an institutional review board, or purchase from a commercial source [eg, AllCells Save | Full Paper | |
2006 | Nudera, J; | Mechanical stresses elicit osteogenic changes in HMSCS encapsulated in a polyethylene glycol scaffold | Thesis | The samples of fresh bone marrow (Allcells, Berkeley, CA) were delivered in 10ml amoimts. Both of the patients tested negative for human immunodeficiency virus (HIV), hepatitis B Save | Full Paper | |
2006 | Crot, CA; | Probing the solid-liquid interface of synthetic biomaterials via x-ray scattering techniques | Thesis | The general biocompatibility of the allylamine films is compared with that of the native oxide surface of Ti by culturing human mesenchymal stems cells (hMSCs) isolated from healthy donors (AllCells, Berkeley, CA) (82). HMSCs are plated in monolayer | Full Paper | |
2006 | Lee, CH;Moioli, EK;Mao, JJ; | Fibroblastic differentiation of human mesenchymal stem cells using connective tissue growth factor | Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference | 17946857 | Human bone marrow samples taken from healthy donors ranging from 18 to 45 years of age were purchased from AllCells (Berkeley, CA). The bone marrows were prepared to isolate hMSCs per our previous methods [10], [11]. Briefly, the whole bone marrow sa | Full Paper |
2006 | Moinnes, JJ;Vidula, N;Halim, N;Othman, SF; | Ultrasound accelerated bone tissue engineering monitored with magnetic resonance microscopy | Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference | 17946836 | Mesenchymal stem cells (MSCs) isolated from fresh adult human bone marrow were provided by AllCells (AllCells LLC, Berkeley, CA). Nucleated cells were expanded via incubation Save | Full Paper |
2006 | Dravid, G;Hammond, H;Cheng, L; | Culture of human embryonic stem cells on human and mouse feeder cells | Methods in molecular biology (Clifton, N.J.) | 16881511 | mitomycin C-treated (Speciality Media; cat. no. PMEF-CF [mitomycin-treated]) (see Note 5 ). 3. Normal breast feeder cells: CCD-1087sk (American Type Culture Collection, Manassas, VA; cat. no. CRL-2104). 4. Bone marrow mononuclear cells (AllCells; L | Full Paper |
2006 | MacDonald, M; | The DataGridView | Pro .NET 2.0 Windows Forms and Custom Controls in C# | Similar to AllCells, except the DataGridView only considers the rows that are currently visible at the time the property is set. This option is used to improve performance for large grids. Save | Full Paper | |
2006 | Crossman, LC; | Predictors of imatinib response in patients with chronic myeloid leukaemia | Thesis | allowing us to compare the groups pre-imatinib. All patients consented to enter this study (Appendix 2). Four samples of healthy BM MNC's were obtained from AllCells (Berkeley, CA), to act as normal controls | Full Paper | |
2006 | Chen, C; | Megakaryocytic* transcriptional program and developmental plasticity | Thesis | Mk and G cell cultures were initiated with fresh G-CSF-mobilized human peripheral blood CD34+ cells from normal donors (AllCells; Berkeley, CA). For Mk cultures, cells were cultured in X-VIVO 20 serum-free medium (BioWhittaker; Walkersville, MD) sup | Full Paper | |
2006 | Yourek, G; | Effects of differentiation and shears stress on stem cell gene expression and nanomechanics | Thesis | Whole bone marrow (AllCells, Berkeley, CA) was mixed with "RosetteSep®" (StemCell Technologies; Vancouver, BC) (50 pL per 1 mL of bone marrow) and incubated for 20 minutes at room temperature (RT). The "RosetteSep®" antibody cocktail crosslinks unwan | Full Paper | |
2006 | Clark, PA; | Modulating bone ingrowth into titanium implants by growth factor delivery and mechanical loading | Thesis | Human mesenchymal stem cell isolation and expansion Human mesenchymal stem cells (hMSCs) were isolated from bone marrow. Whole human bone marrow was purchased commercially (AllCells LLC, Berkeley, CA). Negative selection (RosetteSep, StemCell | Full Paper | |
2006 | Charoonpatrapong, K; | The roles of nuclear matrix protein 4 and high mobility group box 1 in mediating the osteoblast response to anabolic agents | Thesis | Human cord blood-derived osteoclastogenesis was performed as described previously (Fuson & Galvin, 2002) with slight modification. Frozen human cord blood mononuclear cells (CBMNCs) were purchased from AllCells (Berkeley, CA). CBMNCs were quickly tha | Full Paper | |
2005 | De Vry, CG;Valdez, M;Lazarov, M;Muhr, E;Buelow, R;Fong, T;Iyer, S; | Topical application of a novel immunomodulatory peptide, RDP58, reduces skin inflammation in the phorbol ester-induced dermatitis model | J. Invest. Dermatol. | 16117788 | 10% neutral-buffered formalin (Fisher Scientific). The preparation of tissues by embedding in paraffin, sectioning, and staining was carried out by QualTek Molecular Laboratories (Santa Barbara, California). A series of ear cross | Full Paper |
2005 | Finnberg, N;Gruber, JJ;Fei, P;Rudolph, D;Bric, A;Kim, SH;Burns, TF;Ajuha, H;Page, R;Wu, GS;Chen, Y;McKenna, WG;Bernhard, E;Lowe, S;Mak, T;El-Deiry, WS; | DR5 knockout mice are compromised in radiation-induced apoptosis | Mol. Cell. Biol. | 15713653 | ... ts separated on a 2% agarose gel. Mean band density was determined with NIH Image 1.62 and normalized to the band density of 18S rRNA. IN SITU HYBRIDIZATION. In situ hybridization was performed by Phylogeny Inc. as described (17). Briefly, fet | Full Paper |
2005 | Schirmer, A;Gadkari, R;Reeves, CD;Ibrahim, F;DeLong, EF;Hutchinson, CR; | Metagenomic analysis reveals diverse polyketide synthase gene clusters in microorganisms associated with the marine sponge Discodermia dissoluta | Appl. Environ. Microbiol. | 16085882 | We thank Dan Morse (University of California, Santa Barbara) and David Myles for obtaining Discodermia dissoluta, Steve Bernstein (QualTek Molecular Laboratories, Santa Barbara, CA) for help with microscopy on sponge thin sections, and Ralph Reid for | Full Paper |
2005 | Pyle-Chenault, RA;Stolk, JA;Molesh, DA;Boyle-Harlan, D;McNeill, PD;Repasky, EA;Jiang, Z;Fanger, GR;Xu, J; | VSGP/F-spondin: a new ovarian cancer marker | Tumour Biol. | 16103746 | Rockford, Ill., USA). Immunohistochemistry was performed with affinity-purified rabbit polyclonal antibody on formalin-fixed, paraffin-embedded tissues by QualTek Molecular Laboratories (Santa Barbara, Calif., USA). Steam heat | Full Paper |
2005 | Wang, B;Berger, M;Masters, G;Albone, E;Yang, Q;Sheedy, J;Kirksey, Y;Grimm, L;Wang, B;Singleton, J;Soltis, D; | Radiotherapy of human xenograft NSCLC tumors in nude mice with a 90Y-labeled anti-tissue factor antibody | Cancer Biother. Radiopharm. | 15989475 | An IHC study of TF expression in human tissues was performed at QualTek Labs (Newton, PA). Briefly, formalin-fixed, paraffin-embedded sec- tions from human NSCLC tumors and different normal human tissues were taken from QualTek's human tissue bank | Full Paper |
2005 | Urnov, FD;Miller, JC;Lee, YL;Beausejour, CM;Rock, JM;Augustus, S;Jamieson, AC;Porteus, MH;Gregory, PD;Holmes, MC; | Highly efficient endogenous human gene correction using designed zinc-finger nucleases | Nature | 15806097 | Human CD4+ T cells (AllCells) were grown according to the supplier's instructions. Cells were activated using 20 Units per ml IL-2 (R&D Systems) and αCD3/CD28 beads (Miltenyi Biotec, T Cell Activation/Expansion kit), and transfected with ZFN and dono | Full Paper |
2005 | Freud, AG;Becknell, B;Roychowdhury, S;Mao, HC;Ferketich, AK;Nuovo, GJ;Hughes, TL;Marburger, TB;Sung, J;Baiocchi, RA;Guimond, M;Caligiuri, MA; | A human CD34(+) subset resides in lymph nodes and differentiates into CD56bright natural killer cells | Immunity | 15780987 | Fresh human BM was donated or purchased from AllCells, LLC (Berkeley, CA) and was received within 24 hr of harvest. PB leukopaks were obtained from the American Red Cross. BM Save | Full Paper |
2005 | Neviani, P;Santhanam, R;Trotta, R;Notari, M;Blaser, BW;Liu, S;Mao, H;Chang, JS;Galietta, A;Uttam, A;Roy, DC;Valtieri, M;Bruner-Klisovic, R;Caligiuri, MA;Bloomfield, CD;Marcucci, G;Perrotti, D; | The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABL-regulated SET protein | Cancer cell | 16286244 | The oncogenic BCR/ABL kinase activity induces and maintains chronic myelogenous leukemia (CML). We show here that, in BCR/ABL-transformed cells and CML blast crisis (CML-BC) Save | Full Paper |
2005 | Crossman, LC;Druker, BJ;Deininger, MW;Pirmohamed, M;Wang, L;Clark, RE; | hOCT 1 and resistance to imatinib | Blood | 16033955 | mononuclear cells (MNCs) cryopreserved immediately prior to imatinib therapy, and all NRs had BM MNCs stored after 9 to 15 months of imatinib treatment. Four normal BM MNC samples were obtained from AllCells (Berkeley, CA) | Full Paper |
2005 | Corbin, AS;Demehri, S;Griswold, IJ;Wang, Y;Metcalf, CA;Sundaramoorthi, R;Shakespeare, WC;Snodgrass, J;Wardwell, S;Dalgarno, D;Iuliucci, J;Sawyer, TK;Heinrich, MC;Druker, BJ;Deininger, MW; | In vitro and in vivo activity of ATP-based kinase inhibitors AP23464 and AP23848 against activation-loop mutants of Kit | Blood | 15746079 | Mononuclear cells from 4 independent commercially available normal bone marrow samples (AllCells, Berkeley, CA) were cultured in duplicate plates with doses of AP23464 ranging Save;Hematopoietic colony forming assays: Mononuclear cells from four in | Full Paper |
2005 | Orita, T;Tsunoda, H;Yabuta, N;Nakano, K;Yoshino, T;Hirata, Y;Ohtomo, T;Nezu, J;Sakumoto, H;Ono, K;Saito, M;Kumagai, E;Nanami, M;Kaneko, A;Yoshikubo, T;Tsuchiya, M; | A novel therapeutic approach for thrombocytopenia by minibody agonist of the thrombopoietin receptor | Blood | 15374889 | CD34-positive cells (AllCells, LLC, Berkeley, CA) derived from human bone marrow were cultured with rhTPO or VB22B sc(Fv)2 in the presence of 0.2 ng/mL human IL-3 (PeproTech, London, UK) for 11 days at 37°C in Iscove's Modified Dulbecco's Medium (MDM | Full Paper |
2005 | Choi, J;Simons, B;Riley, C;Klinkhammer, T;Cooke, L;Spier, C;Sud, R;Hehn, S;Garewal, H;Mahadevan, D; | Transcriptosome Profiling of B-CLL Identifies WNT-3A and ROR-1 as an Autocrine Mechanism in Cell Survival. | Blood | Full Paper | ||
2005 | Clark, RA;Yamanaka, K;Bai, M;Dowgiert, R;Kupper, TS; | Human skin cells support thymus-independent T cell development | The Journal of clinical investigation | 16224538 | For IFN-γ treated matrices, 50 ng/ml IFN-γ (R&D Systems) was included in the culture medium from plating until day 6. On day 7, 10,000 normal human bone marrow AC133+ precursor cells (AllCells) were added to each matrix, and the unit was cultured in | Full Paper |
2005 | Pan, F;Means, AR;Liu, JO; | Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activation | The EMBO journal | 15902271 | Human peripheral blood mononuclear cells (PMBCs) were obtained from AllCells (Berkeley, CA). Naïve CD4+ T cells were purified by depletion of memory T cells as well as non-CD4+ cells, followed by positive selection with magnetic activated cell separa | Full Paper |
2005 | O'Hare, T;Walters, DK;Stoffregen, EP;Sherbenou, DW;Heinrich, MC;Deininger, MW;Druker, BJ; | Combined Abl inhibitor therapy for minimizing drug resistance in chronic myeloid leukemia: Src/Abl inhibitors are compatible with imatinib | Clinical cancer research : an official journal of the American Association for Cancer Research | 16203792 | Samples were obtained with informed consent from four CML patients or from a tissue repository (AllCells, Berkeley, CA). The CML samples were screened for kinase domain mutations Save | Full Paper |
2005 | Kikuchi, J;Ozaki, H;Nonomura, C;Shinohara, H;Iguchi, S;Nojiri, H;Hamada, H;Kiuchi, A;Nakamura, M; | Transfection of antisense core 2 beta1,6-N-acetylglucosaminyltransferase-1 cDNA suppresses selectin ligand expression and tissue infiltration of B-cell precursor leukemia cells | Leukemia | 16179912 | (CB) was drawn from umbilical cords of cesarean section and normal full-term deliveries. Mononuclear cells (MNCs) were enriched by Ficoll density gradients and subjected to analysis. Bone marrow (BM) derived MNCs were purchased from AllCells (Berkele | Full Paper |
2005 | D'Costa, J;Chaudhuri, S;Civin, CI;Friedman, AD; | CBFbeta-SMMHC slows proliferation of primary murine and human myeloid progenitors | Leukemia | 15815715 | Human cord blood CD34+ cells (AllCells Inc., San Mateo, CA, USA) or cryopreserved human mobilized peripheral blood CD34+ cells from the Johns Hopkins cell bank (from donors who gave informed consent on an IRB-approved protocol) were placed directly i | Full Paper |
2005 | Leugers, S;Allison, D;Pronold, B;Rennebeck, G;Van Zant, G;Tario, J;Swartzwelder, F;Donahue, L; | Stemline Hematopoietic Stem Cell Expansion Medium, a Serum-Free Medium for the Expansion of CD34+ Hematopoietic Stem Cells and Progenitors | Animal Cell Technology Meets Genomics | Cell Preparation. For all experiments, cryopreserved, human CD34+ cells were obtained from independent suppliers (Stemgenix; AllCells, LLC) and were handled in a manner consistent with the supplier’s instructions with regard to storage and reconstitu | Full Paper | |
2005 | Wang, R;Arevalo-Herrera, M;Gardner, MJ;Bonelo, A;Carlton, JM;Gomez, A;Vera, O;Soto, L;Vergara, J;Bidwell, SL;Domingo, A;Fraser, CM;Herrera, S; | Immune responses to Plasmodium vivax pre-erythrocytic stage antigens in naturally exposed Duffy-negative humans: a potential model for identification of liver-stage antigens | European journal of immunology | 15864779 | vivax blood-stage parasites, 2 were living in California and their PBMC were purchased from AllCells (AllCells, LLC, Berkeley, CA) and used in this study as controls. All volunteers Save | Full Paper |
2005 | Mahadevan, D;Spier, C;Della Croce, K;Miller, S;George, B;Riley, C;Warner, S;Grogan, TM;Miller, TP; | Transcript profiling in peripheral T-cell lymphoma, not otherwise specified, and diffuse large B-cell lymphoma identifies distinct tumor profile signatures | Molecular cancer therapeutics | 16373702 | Control RNA was obtained from normal peripheral B cells (AllCells, Berkeley, CA), normal peripheral T cells (AllCells), and a snap-frozen normal lymph node (Department of Pathology, Save | Full Paper |
2005 | Siatskas, C;Underwood, J;Ramezani, A;Hawley, RG;Medin, JA; | Specific pharmacological dimerization of KDR in lentivirally transduced human hematopoietic cells activates anti-apoptotic and proliferative mechanisms | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 16076962 | CD34 + -selected cord blood cells were purchased from AllCells (Berkeley, CA). Cells were maintained at 37C in a humidified atmosphere of 5% CO 2 . Addition of rh stem cell factor ( Save | Full Paper |
2005 | Rao, PE;Petrone, AL;Ponath, PD; | Differentiation and expansion of T cells with regulatory function from human peripheral lymphocytes by stimulation in the presence of TGF-{beta} | Journal of immunology (Baltimore, Md. : 1950) | 15661903 | For T cell differentiation using human cord blood naive CD4 + T cells, frozen CD4 + CD45RA + cells isolated using magnetic beads were obtained from Allcells. Cells were thawed and Save | Full Paper |
2005 | Casida, JE;Quistad, GB; | Serine hydrolase targets of organophosphorus toxicants | Chemico-biological interactions | 16243304 | Whole human blood from seven subjects and lymphocytes (isolated by aphaeresis using a leukopac filter) from three subjects were obtained from AllCells LLC (Emeryville, CA). Save | Full Paper |
2005 | Patel, S;Mehta-Damani, A;Shu, H;Le Pecq, JB; | An analysis of variability in the manufacturing of dexosomes: implications for development of an autologous therapy | Biotechnology and bioengineering | 16037986 | Peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers consisting of 111 leukopacks were obtained from AllCells Inc. (Berkeley, CA), under a protocol approved Save | Full Paper |
2005 | Hong, L;Peptan, I;Clark, P;Mao, JJ; | Ex vivo adipose tissue engineering by human marrow stromal cell seeded gelatin sponge | Annals of biomedical engineering | 15909657 | Human bone MSCs were isolated from fresh bone marrow of adult males and provided commercially by AllCells (AllCells Limited Liability Company, CA.). Nucleated cells of the fresh bone marrow were counted under inverted microscope, plated at a density | Full Paper |
2005 | Murmann, AE;Gao, J;Encinosa, M;Gautier, M;Peter, ME;Eils, R;Lichter, P;Rowley, JD; | Local gene density predicts the spatial position of genetic loci in the interphase nucleus | Experimental cell research | 16202404 | Primary human bone marrow cells (CD34+ and CD33+) were purchased from AllCells Inc. (Berkeley, CA). Chinese muntjac fibroblasts, isolated from tissue of a stillbirth Chinese muntjac fawn provided by the University of Bielefeld, Department of Animal B | Full Paper |
2005 | Alhadlaq, A;Tang, M;Mao, JJ; | Engineered adipose tissue from human mesenchymal stem cells maintains predefined shape and dimension: implications in soft tissue augmentation and reconstruction | Tissue engineering | 15869434 | Human MSCs (hMSCs) were isolated from whole bone marrow aspirates of the iliac crest of a healthy 22- year-old male donor (AllCells, Berkeley, CA). Briefly, cells were enriched by adding RosetteSep mesenchymal enrichment cocktail (StemCell Technologi | Full Paper |
2005 | Marion, N;Liang, W;Liang, W;Reilly, G;Day, D;Rahaman, M;Mao, J; | Borate Glass Supports the In Vitro Osteogenic Differentiation of Human Mesenchymal Stem Cells | Mechanics of Advanced Materials and Structures | Bone marrow samples taken from healthy donors ranging from 18 to 45 years of age were purchased from AllCells (Berkeley, CA). Human bone marrow derived mesenchymal stem Save | Full Paper | |
2005 | Lesniak, MS;Kelleher, E;Pardoll, D;Cui, Y; | Targeted gene therapy to antigen-presenting cells in the central nervous system using hematopoietic stem cells | Neurological research | 16354542 | Human G-CSF-mobilized peripheral blood or cord blood CD34z cells were obtained from AllCells (San Mateo, CA). CD34z cells were purified by immunomagnetic selection (Miltenyi Save | Full Paper |
2005 | Ivanov, M;Kraiko, A; | Numerical solution of the direct-problem of mixed nozzle flow | Fluid Dynamics | Finally, P_I/i, k-1/2 and P-1/2, k-1/2 are determined from the resulting u and v and the given (constant for allcells) entropy and stagnationenthalpy. The calculations showed that the Save | Full Paper | |
2005 | Leung, G;Tang, H;McGuinness, R;Verdonk, E;Michelotti, J;Liu, V; | Cellular Dielectric Spectroscopy: A Label-Free Technology for Drug Discovery | JALA: Journal of the Association for Laboratory Automation | HeLa (CCL-2), and U937 (CRL-1593.2)53 were obtained from ATCC (Manassas, VA). Primary neutrophils were purchased from AllCells, LLC (Berkeley, CA). HeLa cells were grown in minimal essential medium (MEM); U937 cells were grown in RPMI-1640; CHOm1 cel | Full Paper | |
2005 | Owens, BM;Hawley, RG;Spain, LM; | Retroviral transduction in fetal thymic organ culture | Methods in molecular medicine | 15492404 | samples are also available from several commercial sources, for example, AllCells, (Berkeley, CA) or Cambrex BioScience Walkersville, (Walkersville, MD). The thymocyte progenitor population can be transduced with retroviral constructs using VSV-G-pse | Full Paper |
2005 | Varma, C; | Quantitative isoforrm profiling & isoform convergence | Thesis | Samples of human peripheral blood purified B cells, human cord blood purified B cells, and human adult whole bone marrow cells derived from normal (i.e. nondiseased) individuals were purchased from ALLCELLS, LLC, Berkeley, California USA. Each sample | Full Paper | |
2005 | Gao, J; | Quantitative Analysis of Chromosome/Gene Spatial Distribution | Experimental Cell Research | CD34+ and CD33+ cells were primary hematopoietic stem cells and myeloid cells, respectively, (purchased from AllCells, LLC., CA) isolated from bone marrow with a MACS kit, purity of Save | Full Paper | |
2005 | Gilison, D; | The role of telomerase expression in normal development and cancer | Thesis | Fresh human bone marrow was obtained with informed consent from healthy volunteers (AllCells, and Department of Bone Marrow Transplantation, Stanford University School of Medicine). Fresh human cord blood was obtained with informed consent (Departmen | Full Paper | |
2005 | Windgassen, D; | Der technischen Fakultaet der Universitaet Erlangen-Nuernberg zur Erlangung des Grades | Thesis | Healthy-donor PBMCs (AllCells, Berkeley, CA) were used to select T-cell subsets (CD4(+) and CD8(+) negative selections, Miltenyi Biotech, BergischGladbach, Germany). Briefly, non-CD4(+), CD8(+), i.e. CD4(+) or CD8(+) T cells, γ/δ T cells, B cells, NK | Full Paper | |
2004 | Douglas, SA;Naselsky, D;Ao, Z;Disa, J;Herold, CL;Lynch, F;Aiyar, NV; | Identification and pharmacological characterization of native, functional human urotensin-II receptors in rhabdomyosarcoma cell lines | Br. J. Pharmacol. | 15210573 | Search for more papers by this author. Frank Lynch QualTek Molecular Laboratories Inc., Newtown, PA 18940, USA Search for more papers by this author. Frank Lynch QualTek Molecular Laboratories Inc., Newtown, PA 18940, USA ;nch Frank 2 Aiyar Na | Full Paper |
2004 | Bikle, DD;Chang, S;Crumrine, D;Elalieh, H;Man, MQ;Choi, EH;Dardenne, O;Xie, Z;Arnaud, RS;Feingold, K;Elias, PM; | 25 Hydroxyvitamin D 1 alpha-hydroxylase is required for optimal epidermal differentiation and permeability barrier homeostasis | J. Invest. Dermatol. | 15102089 | Peroxidase activity was revealed with DAB substrate (QualTek Laboratories, Santa Barbara, California) followed by counterstaining with methyl green or hematoxylin. Omitting the first antibodies resulted in no signal, indicating the specificity of imm | Full Paper |
2004 | Yu, J;He, S;Friedman, JS;Akimoto, M;Ghosh, D;Mears, AJ;Hicks, D;Swaroop, A; | Altered expression of genes of the Bmp/Smad and Wnt/calcium signaling pathways in the cone-only Nrl-/- mouse retina, revealed by gene profiling using custom cDNA microarrays | J. Biol. Chem. | 15292180 | The labeled transcripts were subjected to alkali hydrolysis (to give an average size of 70 nucleotides) and then hybridized overnight to 5- to 7-μm serial sections of adult mouse retina using standard protocols (Phylogeny Inc., Columbus, OH) | Full Paper |
2004 | Lynch, F; | The use of an interactive computerized daily schedule in a busy interventional radiology practice increases efficiency | J Am Coll Radiol | 17411739 | Full Paper | |
2004 | Kalos, M;Askaa, J;Hylander, BL;Repasky, EA;Cai, F;Vedvick, T;Reed, SG;Wright, GL;Fanger, GR; | Prostein expression is highly restricted to normal and malignant prostate tissues | Prostate | 15176054 | We thank Patrick Roche at the Mayo Clinic and Frank Lynch at Qualtek for performing immunohistochemistry experiments and Masazumi Matsamura for initial work on the generation of the 10E3-G4-D3 monoclonal antibody | Full Paper |
2004 | Tasheva, ES;Ke, A;Conrad, GW; | Analysis of the expression of chondroadherin in mouse ocular and non-ocular tissues | Mol. Vis. | 15332017 | mmol, Amersham Biosciences Corp., Piscataway, NJ). For the ISH experiments, custom service from Phylogeny Inc. (Co- lumbus, OH) was used as previously described [30]. Antibodies: A polyclonal antibody against mouse | Full Paper |
2004 | Tasheva, ES;Ke, A;Deng, Y;Jun, C;Takemoto, LJ;Koester, A;Conrad, GW; | Differentially expressed genes in the lens of mimecan-null mice | Mol. Vis. | 15215744 | secondary antibody was reacted with avidin-biotin-peroxidase complex solution (Vector Laboratories Inc., Burlingame, CA), with immunoreactivity developed in 3,3'-diaminobenzidine (DAB). In situ hybridization: For the ISH experiments, the cus- tom ser | Full Paper |
2004 | Seo, BM;Miura, M;Gronthos, S;Bartold, PM;Batouli, S;Brahim, J;Young, M;Robey, PG;Wang, CY;Shi, S; | Investigation of multipotent postnatal stem cells from human periodontal ligament | Lancet (London, England) | 15246727 | BMSSCs were obtained from a commercially available resource (AllCells LLC, Berkeley, CA, USA). All primary cells used in this study were at 2-4 passages. For each experiment, the Save | Full Paper |
2004 | Griswold, IJ;Shen, LJ;La Rosée, P;Demehri, S;Heinrich, MC;Braziel, RM;McGreevey, L;Haley, AD;Giese, N;Druker, BJ;Deininger, MW; | Effects of MLN518, a dual FLT3 and KIT inhibitor, on normal and malignant hematopoiesis | Blood | 15242881 | Bone marrow mononuclear cells (5 × 104) obtained either commercially (n = 3) (AllCells, San Diego, CA) or from a volunteer donor (n = 1) were plated in methylcellulose media containing 50 ng/mL recombinant human (rh) stem cell factor, 10 ng/mL granul | Full Paper |
2004 | Geddis, AE;Kaushansky, K; | Megakaryocytes express functional Aurora-B kinase in endomitosis | Blood | 15130946 | stimulated donors were purchased (Cambrex, East Rutherford, NJ, or AllCells, Berkley, CA). Cells were thawed according to the manufacturer's directions and cultured for 10 to 14 days in StemPro (Invitrogen) containing recombinant human TPO (rhTPO; 50 | Full Paper |
2004 | Davis, BM;Humeau, L;Slepushkin, V;Binder, G;Korshalla, L;Ni, Y;Ogunjimi, EO;Chang, LF;Lu, X;Dropulic, B; | ABC transporter inhibitors that are substrates enhance lentiviral vector transduction into primitive hematopoietic progenitor cells | Blood | 15059841 | (> 98% purity) derived from adult bone marrow, adult granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood, and cord blood were purchased from AllCells (Berkeley, Save | Full Paper |
2004 | Lu, SJ;Li, F;Vida, L;Honig, GR; | CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic gene expression pattern | Blood | 14962900 | cells were obtained. Normal human BM was purchased from AllCells (Berkeley, CA); CD34+CD38- cells were purified as described previously in this paragraph. Figure 1 shows the fluorescence-activated cell sorter (FACS) profiles that define the gates for | Full Paper |
2004 | Morrissey, P;George, T;Basiji, D;Frost, K;Ortyn, W;Williams, D; | Cell Classification in Human Peripheral Blood Using the Amnis ImageStream® System. | Blood | Human peripheral blood was obtained from AllCells (Berkeley, CA). Whole blood was stained for 15 minutes on ice with FITC conjugated anti-CD45 mAb and PE conjugated anti-CD14 Save | Full Paper | |
2004 | Mahadevan, D;Spier, C;Croce, K;Miller, S;George, B;Riley, C;Warner, S;Grogan, T;Miller, T; | Gene Expression Profiling of Peripheral T-Cell Lymphoma (PTCL, NOS) and Comparison to Diffuse Large B-Cell Lymphoma (DLBCL). | Blood | Full Paper | ||
2004 | Masubuchi, N;May, RD;Atsumi, R; | A predictive model of human myelotoxicity using five camptothecin derivatives and the in vitro colony-forming unit granulocyte/macrophage assay | Clinical cancer research : an official journal of the American Association for Cancer Research | 15475463 | Cells were then washed in IMDM/5 and adjusted to the appropriate cell concentration. Human marrow samples were obtained from AllCells, LLC (Berkeley, CA) from donors that had given informed consent. The age of the 3 donors ranged from 20 to 45 years. | Full Paper |
2004 | Ibañez, V;Sharma, A;Buonamici, S;Verma, A;Kalakonda, S;Wang, J;Kadkol, S;Saunthararajah, Y; | AML1-ETO decreases ETO-2 (MTG16) interactions with nuclear receptor corepressor, an effect that impairs granulocyte differentiation | Cancer research | 15231665 | Retrovirus-containing supernatant was added to human cord blood CD34-positive cells (AllCells) in retronectin (TaKaRa)-coated plates. The cord blood cells were cultured in Iscove's Save | Full Paper |
2004 | Georgantas, RW;Tanadve, V;Malehorn, M;Heimfeld, S;Chen, C;Carr, L;Martinez-Murillo, F;Riggins, G;Kowalski, J;Civin, CI; | Microarray and serial analysis of gene expression analyses identify known and novel transcripts overexpressed in hematopoietic stem cells | Cancer research | 15231652 | The human CD34 + /CD38 − /Lin − cell subset, comprising ∼1-10% of the CD34 + cell population, contains few of the less primitive hematopoietic (lineage-committed) progenitor cells ( Save | Full Paper |
2004 | Humeau, LM;Binder, GK;Lu, X;Slepushkin, V;Merling, R;Echeagaray, P;Pereira, M;Slepushkina, T;Barnett, S;Dropulic, LK;Carroll, R;Levine, BL;June, CH;Dropulic, B; | Efficient lentiviral vector-mediated control of HIV-1 replication in CD4 lymphocytes from diverse HIV+ infected patients grouped according to CD4 count and viral load | Molecular therapy : the journal of the American Society of Gene Therapy | 15194057 | Peripheral blood from healthy donors was obtained from AllCells (Berkeley, CA, USA), and uninfected or HIV+ study subject PBMCs were isolated by Ficoll-Hypaque density gradient separation. Study subjects numbered J23 and higher and U01 had their bloo | Full Paper |
2004 | Davis, BM;Humeau, L;Dropulic, B; | In vivo selection for human and murine hematopoietic cells transduced with a therapeutic MGMT lentiviral vector that inhibits HIV replication | Molecular therapy : the journal of the American Society of Gene Therapy | 14759800 | progenitors were obtained from AllCells (Berkley, CA, USA) in accordance with an IRB protocol obtained by the company and were transduced and cultured in IMDM plus 1× BIT (Stem Cell Technologies, Vancouver, BC, Canada) in 25 ng/ml SCF, Flt-3L, and TP | Full Paper |
2004 | LeCouter, J;Zlot, C;Tejada, M;Peale, F;Ferrara, N; | Bv8 and endocrine gland-derived vascular endothelial growth factor stimulate hematopoiesis and hematopoietic cell mobilization | Proceedings of the National Academy of Sciences of the United States of America | 15548611 | Bv8 and endocrine-gland-derived VEGF (EG-VEGF), or prokineticins, are two highly related, secreted proteins that we previously described as selective angiogenic mitogens. Here we Save | Full Paper |
2004 | Su, AI;Wiltshire, T;Batalov, S;Lapp, H;Ching, KA;Block, D;Zhang, J;Soden, R;Hayakawa, M;Kreiman, G;Cooke, MP;Walker, JR;Hogenesch, JB; | A gene atlas of the mouse and human protein-encoding transcriptomes | Proceedings of the National Academy of Sciences of the United States of America | 15075390 | Tissue Preparation. Human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA), Clontech, AllCells (Berkeley, CA), Clonetics/BioWhittaker (Walk-ersville, MD), AMS Biotechnology (Abingdon, Oxfordshire, U.K.), and t | Full Paper |
2004 | Begovich, AB;Carlton, VE;Honigberg, LA;Schrodi, SJ;Chokkalingam, AP;Alexander, HC;Ardlie, KG;Huang, Q;Smith, AM;Spoerke, JM;Conn, MT;Chang, M;Chang, SY;Saiki, RK;Catanese, JJ;Leong, DU;Garcia, VE;McAllister, LB;Jeffery, DA;Lee, AT;Batliwalla, F;Remmers, E;Criswell, LA;Seldin, MF;Kastner, DL;Amos, CI;Sninsky, JJ;Gregersen, PK; | A missense single-nucleotide polymorphism in a gene encoding a protein tyrosine phosphatase (PTPN22) is associated with rheumatoid arthritis | American journal of human genetics | 15208781 | (GenBank accession number NM_015967) was determined by kinetic RT-PCR analysis by use of total RNA from sorted hematopoeitic cells (AllCells) and selected tissues (Clontech), as described elsewhere (Rogge et al. 2000). Data for additional tissues are | Full Paper |
2004 | Lu, X;Yu, Q;Binder, GK;Chen, Z;Slepushkina, T;Rossi, J;Dropulic, B; | Antisense-mediated inhibition of human immunodeficiency virus (HIV) replication by use of an HIV type 1-based vector results in severely attenuated mutants incapable of developing resistance | Journal of virology | 15194784 | Primary cell isolation, culture, transduction, and challenge. Peripheral blood from healthy donors was obtained from AllCells (Berkeley, Calif.), and lymphocytes were isolated by Ficoll-Hypaque density gradient separation. Cells were resuspended in b | Full Paper |
2004 | Chute, JP;Muramoto, G;Fung, J;Oxford, C; | Quantitative analysis demonstrates expansion of SCID-repopulating cells and increased engraftment capacity in human cord blood following ex vivo culture with human brain endothelial cells | Stem cells (Dayton, Ohio) | 14990859 | Purified human CB CD34+ cells were obtained from AllCells, LLC. (Berkeley, CA; http://www.allcells.com). HUBECs were subcultured at 1 × 105 cells/well in gelatin‐coated 6‐well plates (Costar; Cambridge, MA; http://www.corning.com) as previously descr | Full Paper |
2004 | Courtemanche, C;Huang, AC;Elson-Schwab, I;Kerry, N;Ng, BY;Ames, BN; | Folate deficiency and ionizing radiation cause DNA breaks in primary human lymphocytes: a comparison | FASEB journal : official publication of the Federation of American Societies for Experimental Biology | 14597554 | Normal human T lymphocytes were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% dialyzed Save | Full Paper |
2004 | Friedmann, E;Lemberg, MK;Weihofen, A;Dev, KK;Dengler, U;Rovelli, G;Martoglio, B; | Consensus analysis of signal peptide peptidase and homologous human aspartic proteases reveals opposite topology of catalytic domains compared with presenilins | The Journal of biological chemistry | 15385547 | Microarray Analysis—Human tissues, total RNA samples, and mRNA samples were obtained from Clinomics Biosciences, Inc., Clontech, AllCells, LLC, Clonetics/BioWhittaker, AMS Biotechnology, and the University of California, San Diego. The quality of all | Full Paper |
2004 | Courtemanche, C;Elson-Schwab, I;Mashiyama, ST;Kerry, N;Ames, BN; | Folate deficiency inhibits the proliferation of primary human CD8+ T lymphocytes in vitro | Journal of immunology (Baltimore, Md. : 1950) | 15322179 | Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen, Carlsbad, CA), supplemented with 10% dialyzed Save | Full Paper |
2004 | Chu, PC;Wu, J;Liao, XC;Pardo, J;Zhao, H;Li, C;Mendenhall, MK;Pali, E;Shen, M;Yu, S;Taylor, VC;Aversa, G;Molineaux, S;Payan, DG;Masuda, ES; | A novel role for p21-activated protein kinase 2 in T cell activation | Journal of immunology (Baltimore, Md. : 1950) | 15187108 | Freshly purified PBMC (AllCells, Berkeley, CA) were incubated at 37°C for 2 h to allow the monocytes to adhere to the tissue culture flask. The cells remaining in suspension were recovered and seeded at 106 cells/ml/ well in Yssel’s medium (Gemini Bi | Full Paper |
2004 | Chinnasamy, D;Fairbairn, LJ;Neuenfeldt, J;Treisman, JS;Hanson, JP;Margison, GP;Chinnasamy, N; | Lentivirus-mediated expression of mutant MGMTP140K protects human CD34+ cells against the combined toxicity of O6-benzylguanine and 1,3-bis(2-chloroethyl)-nitrosourea or temozolomide | Human gene therapy | 15319033 | Lentiviral vectors are capable of efficiently transducing nondividing and slowly dividing cells, including hematopoietic stem cells, resulting in stable integration and sustained transgene Save | Full Paper |
2004 | Lu, X;Humeau, L;Slepushkin, V;Binder, G;Yu, Q;Slepushkina, T;Chen, Z;Merling, R;Davis, B;Chang, YN;Dropulic, B; | Safe two-plasmid production for the first clinical lentivirus vector that achieves >99% transduction in primary cells using a one-step protocol | The journal of gene medicine | 15352069 | Normal human blood was obtained from AllCells (Berkely, CA, USA) under the auspices of an IRB-approved protocol supplied by AllCells. Peripheral blood mononuclear cells (PBMCs) Save | Full Paper |
2004 | Kubota, S;Kawata, K;Yanagita, T;Doi, H;Kitoh, T;Takigawa, M; | Abundant retention and release of connective tissue growth factor (CTGF/CCN2) by platelets | Journal of biochemistry | 15598883 | Lower panel: Comparison of the CTGF/CCN2 contents of platelets and other CTGF/CCN2-associated tissues. Total protein extracts of normal human skin and artery were obtained commercially (Cosmo Bio Co., Ltd., Tokyo, Japan), as were human platelets from | Full Paper |
2004 | Mashiyama, ST;Courtemanche, C;Elson-Schwab, I;Crott, J;Lee, BL;Ong, CN;Fenech, M;Ames, BN; | Uracil in DNA, determined by an improved assay, is increased when deoxynucleosides are added to folate-deficient cultured human lymphocytes | Analytical biochemistry | 15183762 | Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and 1% antibiotics (penicillin/streptavidin). To ensure | Full Paper |
2004 | Murray, B;Bennett, B;Sasaki, D; | Analysis of pharmacologic inhibitors of Jun N-terminal kinases | Methods in Enzymology | dom donor buffy coats from the San Diego Blood Bank (San Diego, CA). Neonatal cord blood is obtained from Advanced Bioscience Resources (Alameda, CA) or as selected CD4+ cells (AllCells, Foster City, CA) | Full Paper | |
2004 | Lu, SJ;Li, F;Vida, L;Honig, GR; | CD34CD38 Hematopoietic Precursors Derived from Human Embryonic | researchgate.net | of the total population, and a total of 2,840 CD34+ CD38- cells was obtained. Normal human BM was purchased from AllCells (AllCells, LLC, Berkeley, CA); CD34+ CD38- cells were purified as described above. Figure 1 shows the FACS profiles that define | Full Paper | |
2004 | Murmann, AE; | Topology of genes in mammalian cell nuclei with special emphasis on the MLL gene and its translocation partners | Thesis | ATCC = American Type Culture Collection, Manassas, VA. DSMZ = Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany. 3. Material 38 CD33+) were isolated by and purchased from AllCells Inc. (Berkeley, CA) | Full Paper | |
2004 | Mashiyama, ST; | Folate and other B-vitamins: Effects of deficiency on DNA-uracil content, chromosome damage, homocysteine, and interactions with gene polymorphisms | Thesis | Cell culture and proliferation Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and 1% antibiotics (penic | Full Paper | |
2003 | Winkler, DG;Sutherland, MK;Geoghegan, JC;Yu, C;Hayes, T;Skonier, JE;Shpektor, D;Jonas, M;Kovacevich, BR;Staehling-Hampton, K;Appleby, M;Brunkow, ME;Latham, JA; | Osteocyte control of bone formation via sclerostin, a novel BMP antagonist | EMBO J. | 14633986 | Mouse embryos (15.5 days old) were fixed in 4% phosphate-buffered formaldehyde pH 7.2 overnight, dehydrated and embedded in paraffin. Sections (5 µm thick) were prepared and in situ hybridization performed by Phylogeny, Inc. (Columbus, OH) using [35S | Full Paper |
2003 | Wang, T;Fan, L;Watanabe, Y;McNeill, PD;Moulton, GG;Bangur, C;Fanger, GR;Okada, M;Inoue, Y;Persing, DH;Reed, SG; | L523S, an RNA-binding protein as a potential therapeutic target for lung cancer | Br. J. Cancer | 12644826 | ur studies indicate that L523S may be a valuable addition to the repertoire of cancer-specific targets for the development of new immunotherapeutic and perhaps other therapeutic approaches. We thank Qualtek for their expert IHC analysis. We are grat | Full Paper |
2003 | DuHadaway, JB;Lynch, FJ;Brisbay, S;Bueso-Ramos, C;Troncoso, P;McDonnell, T;Prendergast, GC; | Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: diverse sites of nuclear expression and losses in prostate cancer | J. Cell. Biochem. | 12532338 | Search for more papers by this author. Frank J. Lynch QualTek Molecular Laboratories, Newtown, Pennsylvania. Search for more papers by this author. Shawn Brisbay Search for more papers by this author. Frank J. Lynch QualTek Molecular Laboratories, | Full Paper |
2003 | Tinkelenberg, BA;Fazzone, W;Lynch, FJ;Ladner, RD; | Identification of sequence determinants of human nuclear dUTPase isoform localization | Exp. Cell Res. | 12799180 | 15 min), DUT415 primary antibody incubation (25 min), biotinylated goat anti-mouse IgG secondary antibody (25 min), peroxidase-conjugated ABC-Elite (25 min, Vector Labs, Burlingame, CA, USA), and DAB (diaminobenzidine) chromogen (15 min, QualTek Mole | Full Paper |
2003 | Gibbs, WW; | Untangling the roots of cancer | Sci. Am. | 12840947 | SCIENCE PHOTO LIBRARY; FRANK LYNCH QualTek Molecular Laboratories; ANDREJS LIEPINS/SPL; CNRI/SPL; SPL 1. GROWTH EVEN IN THE ABSENCE OF NORMAL "GO" SIGNALS Most normal cells wait for an external message before dividing | Full Paper |
2003 | Wang, A;Clapper, J;Guderian, JA;Foy, TM;Fanger, GR;Retter, MW;Skeiky, YA; | A novel method for increasing the expression level of recombinant proteins | Protein Expr. Purif. | 12821330 | visualize antigen expression. Slides were counterstained with hematoxylin to visualize cell nuclei. We would like to acknowledge and thank Qualtek for immunohistochemistry results. FACS analysis. HEK293 cells were transiently | Full Paper |
2003 | Weigel-Kelley, KA;Yoder, MC;Srivastava, A; | Alpha5beta1 integrin as a cellular coreceptor for human parvovirus B19: requirement of functional activation of beta1 integrin for viral entry | Blood | 12907437 | Human bone marrow-derived highly purified CD71 + primary erythroid progenitor cells were purchased from AllCells (Berkeley, CA). Recombinant parvovirus B19-Luc vector stocks Save | Full Paper |
2003 | Francisco, JA;Cerveny, CG;Meyer, DL;Mixan, BJ;Klussman, K;Chace, DF;Rejniak, SX;Gordon, KA;DeBlanc, R;Toki, BE;Law, CL;Doronina, SO;Siegall, CB;Senter, PD;Wahl, AF; | cAC10-vcMMAE, an anti-CD30-monomethyl auristatin E conjugate with potent and selective antitumor activity | Blood | 12714494 | and goat-antihuman FITC were obtained from Jackson ImmunoResearch (West Grove, PA). Anti-CD30 mAb Ki-1 was from Accurate Chemicals (Westbury, NY). cBR96, a human chimeric IgG1κ (Seattle Genetics) was used as a chimeric, isotype-matched control. Fresh | Full Paper |
2003 | Glockner, A; | Optimal consistency protocols for replicated files | Eighth Annual International Phoenix Conference on Computers and Communications. 1989 Conference Proceedings | Placement continues until either allcells have been placed, or a number of iterations has beenperformed. If, during cell placement, a valid location for acell cannot be identified, the Save | Full Paper | |
2003 | Lee, J;Elkahloun, AG;Messina, SA;Ferrari, N;Xi, D;Smith, CL;Cooper, R;Albert, PS;Fine, HA; | Cellular and genetic characterization of human adult bone marrow-derived neural stem-like cells: a potential antiglioma cellular vector | Cancer research | 14695205 | RBCs from human adult (donors, 20-40 years old) bone marrow cells purchased from AllCells (Berkely, CA) were depleted by density gradient, and the remaining cells were plated in uncoated tissue culture plastic dishes. After 10 days of culture in 15% | Full Paper |
2003 | Yu, X;Zhan, X;D'Costa, J;Tanavde, VM;Ye, Z;Peng, T;Malehorn, MT;Yang, X;Civin, CI;Cheng, L; | Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cells | Molecular therapy : the journal of the American Society of Gene Therapy | 12788657 | Normal human placental/umbilical CB CD34+ cells, purchased from AllCells (San Mateo, CA), had been purified by immunomagnetic selection (Miltenyi Biotech, Auburn, CA) and were90% CD34+ upon reanalysis by FACS. CB CD34+ cells were cultured at 2.5-5 × | Full Paper |
2003 | Thach, DC;Shaffer, KM;Ma, W;Stenger, DA; | Assessing the feasibility of using neural precursor cells and peripheral blood mononuclear cells for detection of bioactive Sindbis virus | Biosensors & bioelectronics | 12782470 | Fresh normal PBMC were purchased from AllCells , LLC. The cells were received in Dubecco's PBS with 0.5% bovine serum albumin (BSA) and 2 mM EDTA. The cells were spun Save | Full Paper |
2003 | Russell, RS;Roldan, A;Detorio, M;Hu, J;Wainberg, MA;Liang, C; | Effects of a single amino acid substitution within the p2 region of human immunodeficiency virus type 1 on packaging of spliced viral RNA | Journal of virology | 14645555 | Allcells were supplemented with 10% fetal calf serum. Cord bloodmononuclear cells (CBMCs) were grown in RPMI 1640 supplemented with10% fetal calf serum and 20 U of interleukin- Save | Full Paper |
2003 | Cheng, L;Hammond, H;Ye, Z;Zhan, X;Dravid, G; | Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture | Stem cells (Dayton, Ohio) | 12634409 | BM samples collected from healthy and consenting human donors were purchased from AllCells Company (Berkeley, CA; http://www.allcells.com). The use of anonymous primary human cells for laboratory research was approved by the Internal Review Board of | Full Paper |
2003 | Hsu, DH;Paz, P;Villaflor, G;Rivas, A;Mehta-Damani, A;Angevin, E;Zitvogel, L;Le Pecq, JB; | Exosomes as a tumor vaccine: enhancing potency through direct loading of antigenic peptides | Journal of immunotherapy (Hagerstown, Md. : 1997) | 12973033 | CD14+ monocyte-enriched adherent cells from healthy donor leukopacks acquired from Allcells (Berkeley, CA) were cultured in ultrafiltered AIMV medium (Invitrogen) supplemented Save | Full Paper |
2003 | Hofstra, CL;Desai, PJ;Thurmond, RL;Fung-Leung, WP; | Histamine H4 receptor mediates chemotaxis and calcium mobilization of mast cells | The Journal of pharmacology and experimental therapeutics | 12626656 | Human HMC-1 and WEHI-3 cells were purchased from American Type Culture Collection (Manassas, VA). Human CD34 cord blood cells were from AllCells, LLC (Berkeley, CA). Basophil enrichment kit and serum-free medium were from Stem Cells Technologies (Van | Full Paper |
2003 | Greco, NJ;Lee, WR;Moroff, G; | Increased transmigration of G-CSF-mobilized peripheral blood CD34+ cells after overnight storage at 37 degrees C | Transfusion | 14617318 | Samples from UCSD and AllCells LLC were shipped overnight (ON) at 1 to 6C and utilized in experiments upon receipt the following morning (referred to as Day 1). PBPC samples from Save | Full Paper |
2003 | Scherer, A;Krause, A;Walker, JR;Sutton, SE;Serón, D;Raulf, F;Cooke, MP; | Optimized protocol for linear RNA amplification and application to gene expression profiling of human renal biopsies | BioTechniques | 12661160 | Human PBMCs were purchased from Allcells (Oakland, CA, USA). Cells were subjected to RNA purification with RNeasy® (Qiagen, Valencia, CA, USA). The RNA was eluted with 80 µL RNase-free water (Ambion, | Full Paper |
2003 | Onguru, O;Celasun, B;Gunhan, O; | Comparison of DNA ploidy and nuclear morphometric parameters with the conventional prognostic factors in transitional cell carcinomas | The Tohoku journal of experimental medicine | 12703658 | ThemeasurementofNA10in thisstudydemonstratedthemeannucleararea ofthebiggest10cellsamong the allcells measuredatthenucleussuspensionforeach case. Thus, Save | Full Paper |
2003 | Yamaguchi, H;Aridgides, LJ;Zeng, W;Osgood, C;Young, NS;Liu, JM; | Genetic and transcriptional analysis of spindle checkpoint genes in bone marrow failure patients | Blood cells, molecules & diseases | 12737950 | peripheral blood (PB)-MNCs was extracted using an RNAzol kit (Biotex Laboratories, Houston, TX) or Trizol (Life Technologies). Total RNA from purified T cell, B cell, and neutrophil fractions was purchased (AllCells, LLC, Berkeley, CA) | Full Paper |
2003 | Christensen, JL; | Mechanisms and mediators of hematopoietic stem cell fate | Thesis | Human bone marrow was obtained from normal donors (Allcells, Oakland, CA). Bone marrow was diluted in Hanks balanced salt solution, and 20ml was layered onto 15ml of ficoll (Ficoll-Paque PLUS (Amersham Phamacia Biotech), and centrifuged for 30 minute | Full Paper | |
2003 | Chu, P;Pardo, J;Zhao, H;Li, CC;Pali, E;Shen, MM;Qu, K;Yu, SX;Huang, BC;Yu, P;Masuda, ES;Molineaux, SM;Kolbinger, F;Aversa, G;de Vries, J;Payan, DG;Liao, XC; | Systematic identification of regulatory proteins critical for T-cell activation | Journal of biology | 12974981 | 1,599 of its open reading frame; and TRAC-1, base pairs 1-509 of its open reading frame. Culture and infection of primary T lymphocytes Commercially available primary blood mononuclear cells (PBMC; AllCells LLC, Berkeley, USA) were cultured in RPMI | Full Paper |
2003 | Knowles, S;Woods, R;McWhirter, J;McCanny, J; | Bit-level systolic arrays for IIR filtering | [1988] Proceedings. International Conference on Systolic Arrays | throughout this paper.,The application contains four main functions as follows:,1),startPlay,function calls,setup,to set the,dimension of all DOM elements with a class name of,allCells,;,2), Save | Full Paper | |
2003 | Chow, DC; | Effects of surface-immobilized stem cell factor on the differentiation and proliferation of hematopoietic cells | Thesis | gnetic cell separation system (Miltenyl Biotec GmbH, Bergisch Gladbach, Germany) (AllCells, Berkely, CA). Cells were cultured in the presence of soluble SCF (100 ng/ml) or surface-immobilized SCF in serum-free medium (XVIVO20. Cambrex Bio Science, Wa | Full Paper | |
2002 | Elias, PM;Ahn, SK;Denda, M;Brown, BE;Crumrine, D;Kimutai, LK;Kömüves, L;Lee, SH;Feingold, KR; | Modulations in epidermal calcium regulate the expression of differentiation-specific markers | J. Invest. Dermatol. | 12445203 | from Vector Laboratories (Burlingame, CA). Peroxidase activity was revealed with diaminobenzidine (QualTek Laboratories, Santa Barbara, CA), followed by counterstaining with methyl green. Omission of the first antibodies | Full Paper |
2002 | Xie, Z;Komuves, L;Yu, QC;Elalieh, H;Ng, DC;Leary, C;Chang, S;Crumrine, D;Yoshizawa, T;Kato, S;Bikle, DD; | Lack of the vitamin D receptor is associated with reduced epidermal differentiation and hair follicle growth | J. Invest. Dermatol. | 11851870 | Peroxidase activity was revealed with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followed by counterstaining with methyl green or hematoxylin. Omitting the first antibodies resulted in no signal, indicating the specificity o | Full Paper |
2002 | Fanger, GR;Houghton, RL;Retter, MW;Hendrickson, RC;Babcook, J;Dillon, DC;Durham, MD;Reynolds, LD;Johnson, JC;Carter, D;Fleming, TP;Roche, PC;Persing, DH;Reed, SG; | Detection of mammaglobin in the sera of patients with breast cancer | Tumour Biol. | 12499777 | Acknowledgments We thank Frank Lynch at Qualtek for immunohistochemistry efforts and expertise. Work was supported in part by USPHS grant CA76227 (TPF), and NIH grants CA-75794 (RLH) and CA- 86673 (DCD) References 1 Donegan W, Spratt J: Cancer of the | Full Paper |
2002 | Kumar, S;Hanning, CR;Brigham-Burke, MR;Rieman, DJ;Lehr, R;Khandekar, S;Kirkpatrick, RB;Scott, GF;Lee, JC;Lynch, FJ;Gao, W;Gambotto, A;Lotze, MT; | Interleukin-1F7B (IL-1H4/IL-1F7) is processed by caspase-1 and mature IL-1F7B binds to the IL-18 receptor but does not induce IFN-gamma production | Cytokine | 12096920 | Full Paper | |
2002 | Mulero, JJ;Boyle, BJ;Bradley, S;Bright, JM;Nelken, ST;Ho, TT;Mize, NK;Childs, JD;Ballinger, DG;Ford, JE;Rupp, F; | Three new human members of the lipid transfer/lipopolysaccharide binding protein family (LT/LBP) | Immunogenetics | 12185532 | The BPIL2 probe was derived from a 414-nt coding sequence between the primers 5'-CATTATTGCAAGTGAAGTCAAAGC-3' and 5'-ACCATGA- AGGGCTGGGACAAGATG-3'. QualTek Molecular labs (Santa Barbara, Calif.) performed the automated in situ hybridization us- ing a | Full Paper |
2002 | Zhao, Z;Yaar, R;Ladd, D;Cataldo, LM;Ravid, K; | Overexpression of A3 adenosine receptors in smooth, cardiac, and skeletal muscle is lethal to embryos | Microvasc. Res. | 11749073 | Page 4. tion, generating either a sense or an antisense strand template for in vitro transcription (with EcoRV or HindIII, respectively). In situ hybridization was performed by Dr. Adel Mikhail (Phylogeny, Inc., Columbus, OH) as described by Lyons et | Full Paper |
2002 | Hevehan, DL;Miller, WM;Papoutsakis, ET; | Differential expression and phosphorylation of distinct STAT3 proteins during granulocytic differentiation | Blood | 11861277 | Fresh G-CSF-mobilized peripheral blood CD34 + cells from normal donors were obtained from AllCells (Foster City, CA) within 24 hours after collection. The experimental design and Save | Full Paper |
2002 | Cui, Y;Golob, J;Kelleher, E;Ye, Z;Pardoll, D;Cheng, L; | Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cells | Blood | 11781219 | Human G-CSF-mobilized peripheral blood or cord blood CD34+ cells were obtained from AllCells (San Mateo, CA). CD34+ cells were purified by immunomagnetic selection (Miltenyi Biotec, Auburn, CA) and then cryopreserved. The purity of CD34+ cells was us | Full Paper |
2002 | Tuttle, G;Fallahi, S;Abidi, A; | An 8 b CMOS vector A/D converter | 1993 IEEE International Solid-State Circuits Conference Digest of Technical Papers | The two pMOSFETsin allcells are diode connected, and the nMOSFET is biased by a globally-applied reference gate voltage. The input is then switched to the prompt vector X, and the Save | Full Paper | |
2002 | Kang, SJ;Cresswell, P; | Regulation of intracellular trafficking of human CD1d by association with MHC class II molecules | The EMBO journal | 11927549 | DCs were derived from monocytes as described (Sallusto and Lanzavecchia, 1994). Briefly, monocytes from peripheral blood mononuclear cells (8 × 108 cells) (AllCells, LLC, Berkeley, CA) were attached to tissue culture flasks in 20% FBS/RPMI 1640 twice | Full Paper |
2002 | Manz, MG;Miyamoto, T;Akashi, K;Weissman, IL; | Prospective isolation of human clonogenic common myeloid progenitors | Proceedings of the National Academy of Sciences of the United States of America | 12193648 | Bone Marrow and Cord Blood Samples. Fresh human bone marrow (donor age, 20-47 years) was obtained with informed consent from healthy volunteers (AllCells, Berkeley, CA), allogeneic bone marrow donors (Department of Bone Marrow Transplantation, Stanfo | Full Paper |
2002 | Mahmud, DL;G-Amlak, M;Deb, DK;Platanias, LC;Uddin, S;Wickrema, A; | Phosphorylation of forkhead transcription factors by erythropoietin and stem cell factor prevents acetylation and their interaction with coactivator p300 in erythroid progenitor cells | Oncogene | 11896584 | Primary human erythroid progenitors were derived by in vivo culture of CD34+ cells isolated from peripheral blood. Growth factor mobilized peripheral blood collected from normal donors was purchased from AllCells, LLC (Berkeley, CA, USA). These blood | Full Paper |
2002 | Yang, H;Miller, WM;Papoutsakis, ET; | Higher pH promotes megakaryocytic maturation and apoptosis | Stem cells (Dayton, Ohio) | 12110701 | Cultures were initiated with adult human CD34+ cells selected from apheresis products obtained from normal donors (AllCells; Berkeley, CA; http://www.allcells.com) or cancer patients (Northwestern Memorial Hospital, Chicago, IL) following stem cell m | Full Paper |
2002 | Kim, H;Whartenby, KA;Georgantas, RW;Wingard, J;Civin, CI; | Human CD34+ hematopoietic stem/progenitor cells express high levels of FLIP and are resistant to Fas-mediated apoptosis | Stem cells (Dayton, Ohio) | 11897874 | Human placental/umbilical cord blood (CB) CD34+ cell preparations, purified by immunomagnetic selection following manufacturer's instructions (Miltenyi Biotechnologies; Auburn, CA; http://www.miltenyibiotec.com) and then cryopreserved, were purchased | Full Paper |
2002 | Welm, AL;Timchenko, NA;Ono, Y;Sorimachi, H;Radomska, HS;Tenen, DG;Lekstrom-Himes, J;Darlington, GJ; | C/EBPalpha is required for proteolytic cleavage of cyclin A by calpain 3 in myeloid precursor cells | The Journal of biological chemistry | 12105198 | Bone marrow was isolated from both femurs of adult animals as described (25). CD33+ and CD15+ bone marrow cells were obtained from AllCells, L.L.C. skeletal muscle was harvested from adult animals as a control for calpain 3 Northern analysis | Full Paper |
2002 | Jacobs-Helber, SM;Abutin, RM;Tian, C;Bondurant, M;Wickrema, A;Sawyer, ST; | Role of JunB in erythroid differentiation | The Journal of biological chemistry | 11726656 | Growth factor-mobilized peripheral blood collected from normal donors was purchased from AllCells, LLC (Berkeley, CA). CD34 + cells were isolated from growth factor-mobilized Save | Full Paper |
2002 | Harrisson, F; | Cellular origin of the basement membrane in embryonic chicken/quail chimeras. | International Journal of Developmental Biology | such as Quailnucleoli, are not clear in allcells. The drawings show the place where the photomicrographs were taken. The dashed area indicates the locatIOn of the mesoblast. Save | Full Paper | |
2002 | Sharma, A; | The role of the hiwi gene in human hematopoiesis | Thesis | Louis, MO) and stained first with biotin-conjugated K6.1 (20 ug/ml). washed, and labeled with Miltenyi streptavidin-conjugated iron microbeads (Miltenyi Biotec) and selected as described above according to manufacturer's instructions. Cord blood (CB) | Full Paper | |
2001 | Afar, DE;Vivanco, I;Hubert, RS;Kuo, J;Chen, E;Saffran, DC;Raitano, AB;Jakobovits, A; | Catalytic cleavage of the androgen-regulated TMPRSS2 protease results in its secretion by prostate and prostate cancer epithelia | Cancer Res. | 11245484 | We thank Drs. Robert Eisenman, Owen Witte, and William Isaacs for helpful suggestions and critical reading of the manuscript; Frank Lynch and Page Erickson at QualTek Molecular Laboratories for their expertise in immunohistochemistry; and Celeste Jon | Full Paper |
2001 | Xu, J;Kalos, M;Stolk, JA;Zasloff, EJ;Zhang, X;Houghton, RL;Filho, AM;Nolasco, M;Badaró, R;Reed, SG; | Identification and characterization of prostein, a novel prostate-specific protein | Cancer Res. | 11245466 | Immunohistochemical Studies. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissues by QualTek Molecular Laboratories using the prostein-specific mouse monoclonal antibody 10E3- G4-D3. Chromosome Localization. The Gen | Full Paper |
2001 | Wang, T;Fan, L;Watanabe, Y;McNeill, P;Fanger, GR;Persing, DH;Reed, SG; | L552S, an alternatively spliced isoform of XAGE-1, is over-expressed in lung adenocarcinoma | Oncogene | 11753648 | An affinity purified anti-L552S polyclonal antibody was subjected to immunohistochemistry analysis by QualTek Molecular Laboratories (Santa Barbara, CA, USA). In all cases, four micron sections of formalin fixed, paraffin-embedded tissues were used. | Full Paper |
2001 | Lin, H;Ho, AS;Haley-Vicente, D;Zhang, J;Bernal-Fussell, J;Pace, AM;Hansen, D;Schweighofer, K;Mize, NK;Ford, JE; | Cloning and characterization of IL-1HY2, a novel interleukin-1 family member | J. Biol. Chem. | 11278614 | The anti-CD20 monoclonal antibody was purchased from DAKO (Carpenteria, CA), and the anti-Ki67 was obtained from Coulter Immunotech (Miami, FL). Immunohistochemistry was performed at QualTek Molecular Systems, Inc | Full Paper |
2001 | Kutty, RK;Kutty, G;Samuel, W;Duncan, T;Bridges, CC;El-Sherbeeny, A;Nagineni, CN;Smith, SB;Wiggert, B; | Molecular characterization and developmental expression of NORPEG, a novel gene induced by retinoic acid | J. Biol. Chem. | 11042181 | Immunoreactivity was detected by peroxidase staining using reagents from Dako Corp. (Carpinteria, CA). In Situ Hybridization. In situ hybridization was performed by Phylogeny Inc. following the procedure of Lyonset al. (18) as described before (19) | Full Paper |
2001 | Suerbaum, S;Lohrengel, M;Sonnevend, A;Ruberg, F;Kist, M; | Allelic diversity and recombination in Campylobacter jejuni | J. Bacteriol. | 11274115 | Different genes show network-like and tree-like phylogeny in C. jejuni. In order to further analyze the population structure of C. jejuni, we used split decomposition analysis. Split decomposition was originally described by Bandelt and Dress in 1992 | Full Paper |
2001 | Taylor, RE;Shows, KH;Zhao, Y;Pittler, SJ; | A PDE6A promoter fragment directs transcription predominantly in the photoreceptor | Biochem. Biophys. Res. Commun. | 11401494 | Mannheim). An outside commercial company (Qualtek Inc., CA) confirmed results obtained in-house using the same exper- imental probes as above with proprietary in situ hybridization re- agents and the following conditions | Full Paper |
2001 | Ladner, RD; | The role of dUTPase and uracil-DNA repair in cancer chemotherapy | Curr. Protein Pept. Sci. | 12374095 | await further investigation. ACKNOWLEDGEMENTS Special thanks is extended to Dr. Frank J. Lynch at QualTek Molecular Laboratories, Santa Barbara, CA, for expertise in immunohistochemistry. Special thanks are extended | Full Paper |
2001 | Gao, Z;Golob, J;Tanavde, VM;Civin, CI;Hawley, RG;Cheng, L; | High levels of transgene expression following transduction of long-term NOD/SCID-repopulating human cells with a modified lentiviral vector | Stem cells (Dayton, Ohio) | 11359950 | Normal human cord blood (CB) CD34+ cells were purchased from either AllCells (San Mateo, CA; http://www.purecell.com) or Poietics/BioWhittaker (Gaithersburg, MD; http://www.biowhittaker.com/). CD34+ cells were purified by immunomagnetic selection (Mi | |
2001 | Bonfoco, E;Li, E;Kolbinger, F;Cooper, NR; | Characterization of a novel proapoptotic caspase-2- and caspase-9-binding protein | The Journal of biological chemistry | 11350957 | Positively selected, CD19+ MACS-purified human B cells (AllCells, San Jose, CA) were resuspended at 1 × 106 cells/ml in Yssel's medium without antibody (Gemini Bioproducts, Calabasas, CA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT). | |
2001 | Alonso, A;Saxena, M;Williams, S;Mustelin, T; | Inhibitory role for dual specificity phosphatase VHR in T cell antigen receptor and CD28-induced Erk and Jnk activation | The Journal of biological chemistry | 11085983 | Human bone marrow was purchased from AllCells LLC (Foster City, CA). Bone marrow leukocytes were obtained by hypotonic lysis of the erythrocytes in water for 1 min followed by washing of the cells in RPMI medium. Peripheral blood lymphocytes (∼80% T | |
2001 | 西田耕介, ;高城敏美, ;木下進一, ;辻正, ; | 燃料電池・ガスタービン複合発電システムの性能解析 (熱工学, 内燃機関, 動力など) | 日本機械学会論文集 B 編 | 段目の電 のみに蒸気を投入する方式(1段目蒸気投入 方式,1stcel1steamadditionform)は,全 段の電 に蒸 気を投入する方式(全段蒸気投入方式,Allcells Steam additionform)よりも単位燃料 Save | ||
2001 | Mostafa, SS;Papoutsakis, ET;Miller, WM; | Oxygen tension modulates the expression of cytokine receptors, transcription factors, and lineage-specific markers in cultured human megakaryocytes | Experimental hematology | 11438210 | Cultures were initiated with adult human CD34+ cells selected from apheresis products obtained from normal donors mobilized with G-CSF (Poietics Technologies, Gaithersburg, MD, USA, and AllCells, Foster City, CA, USA). CD34+ cells were selected 1 day | |
2000 | Xu, J;Stolk, JA;Zhang, X;Silva, SJ;Houghton, RL;Matsumura, M;Vedvick, TS;Leslie, KB;Badaro, R;Reed, SG; | Identification of differentially expressed genes in human prostate cancer using subtraction and microarray | Cancer Res. | 10749139 | Truncated P503S (amino acid 113–241) was cloned into pET32b (Novagen) and expressed in E. coli as thioredoxin fusion proteins with a histidine tag. P503S was purified by nickel chromatography, digested with thrombin, and further purified by reverse-p | |
2000 | Kömüves, LG;Hanley, K;Lefebvre, AM;Man, MQ;Ng, DC;Bikle, DD;Williams, ML;Elias, PM;Auwerx, J;Feingold, KR; | Stimulation of PPARalpha promotes epidermal keratinocyte differentiation in vivo | J. Invest. Dermatol. | 10951268 | purchased from Vector (Burlingame, CA). Peroxidase activity was revealed with diaminobenzidine (QualTek Laboratories, Santa Barbara, CA), followed by counterstaining with methyl green. Omitting the first antibodies resulted | |
2000 | Johnson, LV;Ozaki, S;Staples, MK;Erickson, PA;Anderson, DH; | A potential role for immune complex pathogenesis in drusen formation | Exp. Eye Res. | 10865992 | ANDERSONa a Center for the Study of Macular Degeneration, Neuroscience Research Institute, University of California, Santa Barbara, CA 93106, USA, b QualTek Molecular Laboratories, Santa Barbara, CA 93111, USA (Received | |
2000 | Yeung, G;Mulero, JJ;McGowan, DW;Bajwa, SS;Ford, JE; | CD39L2, a gene encoding a human nucleoside diphosphatase, predominantly expressed in the heart | Biochemistry | 11041856 | the manufacturer. Automated in situ hybridization was performed by QualTek Molecular labs (Santa Barbara, CA) using a modified version of a previously published procedure (17). The Ventana Medical Systems, Inc. (Tucson | |
2000 | Kralchevsky, PA;Nagayama, K; | Capillary interactions between particles bound to interfaces, liquid films and biomembranes | Advances in colloid and interface science | 10768480 | This dissertation studies cellular rearrangements in tissues and attempts to establish the role of physical properties of cells, tissues and membranes in several biological phenomena. Save | |
2000 | Pettus, P; | Modeling Fecal Bacteria in Oregon Coastal Streams Using Spatially Explicit Watershed Characteristics | Portland State University | Pathogens, such as Escherichia coli and fecal coliforms, are causing the majority of water quality impairments in US, making up∼ 87% of this grouping's violations. Predicting and Save | ||
2000 | Mostafa, SS; | Effects of culture oxygen tension on human megakaryocytes: A phenotypic and mechanistic study | Thesis | All of the molecular studies were conducted using CD34+ cells from fresh apheresis products (~ 2% CD34+ cells) collected from G-CSF mobilized normal donors (Poietics Technologies, Gaithersburg, MD and AllCells, Foster City, CA). PB instead of BM cell | ||
1999 | Hubert, RS;Vivanco, I;Chen, E;Rastegar, S;Leong, K;Mitchell, SC;Madraswala, R;Zhou, Y;Kuo, J;Raitano, AB;Jakobovits, A;Saffran, DC;Afar, DE; | STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumors | Proc. Natl. Acad. Sci. U.S.A. | 10588738 | Human tissues for RNA and protein analyses were obtained from the Human Tissue Resource Center at the University of California, Los Angeles; from the National Disease Research Interchange (Philadelphia); and from QualTek Molecular Labs (Santa Barbara | |
1999 | Kömüves, LG;Hanley, K;Jiang, Y;Katagiri, C;Elias, PM;Williams, ML;Feingold, KR; | Induction of selected lipid metabolic enzymes and differentiation-linked structural proteins by air exposure in fetal rat skin explants | J. Invest. Dermatol. | 10084306 | purchased from Vector (Burlingame, CA). Peroxidase activity was revealed with DAB substrate (QualTek Laboratories, Santa Barbara, CA) followed by counterstaining with methyl green. Omitting the first antibodies resulted | |
1999 | Hu, MC;Wang, YP;Mikhail, A;Qiu, WR;Tan, TH; | Murine p38-delta mitogen-activated protein kinase, a developmentally regulated protein kinase that is activated by stress and proinflammatory cytokines | J. Biol. Chem. | 10066767 | From the ‡ Department of Cell Biology, Amgen, Inc., Thousand Oaks, California 91320, ¶ Phylogeny, Inc., Columbus, Ohio 43212, and the ‖ Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030. Next Section. Abstrac | |
1999 | Widder, E;Johnsen, S;Bernstein, S;Case, J;Neilson, D; | Thin layers of bioluminescent copepods found at density discontinuities in the water column | Marine Biology | Communicated by NH Marcus, Tallahassee EA Widder (8) a S. Johnsen Harbor Branch Oceanographic Institution, Fort Pierce, Florida 34946, USA Fax: 001 (0)561 468-0757 e-mail: widder@hboi.edu SA Bernstein QualTek, Santa Barbara, California 93111, USA JF | ||
1999 | Payre, F;Vincent, A;Carreno, S; | ovo/svb integrates Wingless and DER pathways to control epidermis differentiation | Nature | 10421370 | Similar results were obtained in allcells of the embryonic ventral epidermis tested, using a variety of Gal4 drivers. The denticle production induced by ectopic svb expression does not Save | |
1998 | Weymann, R;Jannuzi, B;Lu, L;Bahcall, J;Bergeron, J;Boksenberg, A;Hartig, G;Kirhakos, S;Sargent, W;Savage, B;Schneider, D;Turnshek, D;Wolfe, A; | TheHubble Space TelescopeQuasar Absorption Line Key Project. XIV. The Evolution of Lyα Absorption Lines in the Redshift Intervalz= 0-1.5 | The Astrophysical Journal | We present the results of an analysis of the rate of evolution of the Lyα absorption lines in the redshift interval 0.0 to~ 1.5 based upon a sample of 987 Lyα absorption lines identified in Save | ||
1997 | Schmidt-Erfurth, U;Diddens, H;Birngruber, R;Hasan, T; | Photodynamic targeting of human retinoblastoma cells using covalent low-density lipoprotein conjugates | British journal of cancer | 9000598 | Firstly, the apoB/E receptor, which is present on allcells, is the main mechanism by which cells gain exogenous cholesterol and can be regulated up or down to attain cholesterol Save | |
1997 | Surinya, KH; | Heme biosynthesis in erythroid cells : transcriptional egulation of the human 5=aminolevulinate synthase 2 gene / a thesis submitted to the University of Adelaide for the degree of Doctor of Philosophy by Katharina Helen Surinya | Thesis | There are two isozymes for ALAS: a housekeeping form expressed in allcells (ALAS1), and an erythroid-specific form (ALAS2) responsible for the higher protoporphyrin synthesis in Save | ||
1996 | Kavanagh, K;Whittaker, PA; | Application of protoplast fusion to the nonconventional yeast | Enzyme and microbial technology | Microbio/. 1983. 129, 337 IL3377 Necas. 0.. Svoboda, A. and Havelkova, M. Mechanism of regen- eration of yeast protoplasts: Formation of the cell wall in %hixmc- ckcwon~~~~es pornbr. Folio. Bio[. 1968, 14, 80-85 Necan. 0. Cell wall synthesis in yeast | ||
1996 | Evers, BM;Zhou, Z;Dohlen, V;Rajaraman, S;Thompson, JC;Townsend, CM; | Fetal and neoplastic expression of the neurotensin gene in the human colon | Annals of surgery | 8651737 | This gene is present in allcells in the body, but, interestingly, it is differentially expressed. As shown in this study, it tends to be expressed in the fetal colon and in the adult small bowel. Save | |
1996 | Salinas, E;Abbott, LF; | A model of multiplicative neural responses in parietal cortex | Proceedings of the National Academy of Sciences of the United States of America | 8876244 | Thesetwo parameterswill either be the same for allcells, in which case we refer to them as m andb, orwill be chosen from Gaussian distributions with fixed means and standard Save | |
1996 | Nwoga, JC;Sniffen, JC;Peña-Rasgado, C;Kimler, VA;Rasgado-Flores, H; | Effect of pentachlorophenol on calcium accumulation in barnacle muscle cells | The Journal of physiology | 9011605 | Allcells were mounted in the experimental chamber in the presence of NaSWOCa (see Methods). Except for the case where the cells remained in NaSWOCa, cells were pre-incubated Save | |
1996 | Kineman, RD;Gettys, TW;Frawley, LS; | Role of guanine nucleotide-binding proteins, Gi alpha 3 and Gs alpha, in dopamine and thyrotropin-releasing hormone signal transduction: evidence for competition and commonality | The Journal of endocrinology | 8778223 | Given that SLO is an invasive agent and could result in cell lysis (approximately 1-2% of allcells lyse following SLO treatment), the RHPA allows for the evaluation ofPRL release from Save | |
1996 | Berry, RM;Berg, HC; | Torque generated by the bacterial flagellar motor close to stall | Biophysical journal | 8968619 | ||
1996 | Goodman, MB;Art, JJ; | Positive feedback by a potassium-selective inward rectifier enhances tuning in vertebrate hair cells | Biophysical journal | 8804626 | ||
1996 | Laughlin, MJ;Christiansen, NP;Herzig, GP;Blumenson, L;Bonney, D;Stewart, CC; | CD34 progenitor cell subset analyses in normal human bone marrow and marrow harvested after intermediate-dose chemotherapy | Cytometry | 8979021 | CD34 + cells exhibiting high side scatter, R8 (to include allcells to theright ofR1)were >95% basophils(Fig. 2f). Most mature basophils exhibit low side scatter because their granules Save | |
1995 | Wang, XJ;Golomb, D;Rinzel, J; | Emergent spindle oscillations and intermittent burst firing in a thalamic model: specific neuronal mechanisms | Proceedings of the National Academy of Sciences of the United States of America | 7777551 | The histograms were obtained by computing the mean bursting rates of allcells averaged over a timeinterval of 10 s. (D) Normalized interburst interval (IBI) histogram (bin = 10 ms) Save | |
1995 | Nooter, K;Boersma, AW;Oostrum, RG;Burger, H;Jochemsen, AG;Stoter, G; | Constitutive expression of the c-H-ras oncogene inhibits doxorubicin-induced apoptosis and promotes cell survival in a rhabdomyosarcoma cell line | British journal of cancer | 7880739 | At 1 JM doxorubicin - a concentration that gives more than 4 log cell kill in a clonogenic assay on the R2NEO cells (Figure 3) - 96 h after drug incubation practically allcells were Save | |
1995 | Hofstadler, SA;Swanek, FD;Gale, DC;Ewing, AG;Smith, RD; | Capillary electrophoresis-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for direct analysis of cellular proteins | Analytical chemistry | 7741217 | The initial cell population has a significant spatial distribution, and it is unlikely that allcells lysed simultaneously, leading to band dispersion greater than that due to the initial spatial Save | |
1995 | Juckett, MB;Balla, J;Balla, G;Jessurun, J;Jacob, HS;Vercellotti, GM; | Ferritin protects endothelial cells from oxidized low density lipoprotein in vitro | The American journal of pathology | 7677189 | In allcells, ferritin synthesis is tightly coupled with the intracellular iron concentration, which presumably prevents expansion of a potentially damaging free iron pool. We have found that Save | |
1995 | Preudhomme, C;Lepelley, P;Lovi, V;Zandecki, M;Cosson, A;Fenaux, P; | T-cell acute lymphoblastic leukemia occurring in the course of B cell chronic lymphocytic leukemia: a case report | Leukemia & lymphoma | 8535207 | In the six cases studied, similar immunoglobulin chain isotypes were found in the CLL and ALLcells, while identical cytogenetic abnormalities were present in CLL and ALL in the Save | |
1994 | Uckun, F;Downing, J;Chelstrom, L;Gunther, R;Ryan, M;Simon, J;Carroll, A;Tuel-Ahlgren, L;Crist, W; | Human t(4;11)(q21;q23) acute lymphoblastic leukemia in mice with severe combined immunodeficiency | Blood | |||
1994 | Shikama, N;Ackermann, R;Brack, C; | Protein synthesis elongation factor EF-1 alpha expression and longevity in Drosophila melanogaster | Proceedings of the National Academy of Sciences of the United States of America | 8183891 | Therefore, regulation of protein synthesis can beconsidered as one key homeostatic function common to allcells. A decrease in the rate ofprotein synthesis with advancing age has been Save | |
1994 | Yao, A;Rubin, H; | A critical test of the role of population density in producing transformation | Proceedings of the National Academy of Sciences of the United States of America | 8052648 | In a competentpopulation, allcells have the capacityto produce some transformed foci if kept under the constraint of confluence and/or reduced serum concentration for extended Save | |
1994 | Ingenbleek, Y;Young, V; | Transthyretin (prealbumin) in health and disease: nutritional implications | Annual review of nutrition | 7946531 | the fteld of both thyroid hormones (175, 176), whose three speciftc carrier proteins act as buffering reservoirs, thereby allowing the uniform distribution of the horн mones among allcells Save | |
1994 | Ferreira, GC; | Mammalian ferrochelatase. Overexpression in Escherichia coli as a soluble protein, purification and characterization | The Journal of biological chemistry | 8308010 | dance of ferrochelatase in mitochondria and its difficulty of Although ferrochelatase is present in allcells, it is predomi- solubilization from the membrane environment (1,2). In this nantly Save | |
1994 | McDonnell, DP;Goldman, ME; | RU486 exerts antiestrogenic activities through a novel progesterone receptor A form-mediated mechanism | The Journal of biological chemistry | 8163495 | In the pri- suggests that estrogen receptor-mediated activation of tranmate uterus, for instance, in addition to functioning an an- scription is not identical in allcells. Since PR-A is not Save | |
1994 | Skiba, PJ;Keesler, GA;Tabas, I; | Interferon-gamma down-regulates the lipoprotein(a)/apoprotein(a) receptor activity on macrophage foam cells. Evidence for disruption of ligand-induced receptor recycling by interferon-gamma | The Journal of biological chemistry | 8083207 | Allcells were then assayed for 5 h 3 (in the absence IFN-y) for lZ5I-r-apo(a) degradation. C,resident mouse peritoneal macrophages werepreincubated for 3 days in DMEM, 10%LPDS, Save | |
1994 | Cazenave, C;Frank, P;Toulme, JJ;Büsen, W; | Characterization and subcellular localization of ribonuclease H activities from Xenopus laevis oocytes | The Journal of biological chemistry | 7929207 | to be primarily concentrated in the germinal drawn from the various attempts to characterize these envesicle, but around of this activity was detected 5% in the zymes further: allcells Save | |
1994 | Carrel, F;Dharmawardhane, S;Clark, AM;Powell-Coffman, JA;Firtel, RA; | Spatial and temporal expression of the Dictyostelium discoideum G alpha protein subunit G alpha 2: expression of a dominant negative protein inhibits proper prestalk to stalk differentiation | Molecular biology of the cell | 8186466 | Using the full-length promoter, aggregates showed uniform staining during aggregation and mound formation, suggesting that Ga2 is expressed in allcells at this stage of development, Save | |
1994 | Aoyagi, M;Furusawa, S;Waga, K;Tsunogake, S;Shishido, H; | Suppression of normal hematopoiesis in acute leukemia: effect of leukemic cells on bone marrow stromal cells and hematopoietic progenitor cells | Internal medicine (Tokyo, Japan) | 7949632 | Effects ofleukemic cells (LC) onbone marrowstromal cells and myeloid progenitor cells (CFUC) were studied in vitro, using LClines with different lineage characteristics. LCand/or LC- Save | |
1994 | Van Hamme, LJ; | Associative and statistical accounts of cue competition in causality judgments | Thesis | This expression equ ally utilizes the inform ation in allcells of the ta b le and re p re sen ts one measure of the tru e contingency between the even ts. Although developed w ith in qu ite Save | ||
1993 | Kohl, NE;Mosser, SD;deSolms, SJ;Giuliani, EA;Pompliano, DL;Graham, SL;Smith, RL;Scolnick, EM;Oliff, A;Gibbs, JB; | Selective inhibition of ras-dependent transformation by a farnesyltransferase inhibitor | Science (New York, N.Y.) | 8316833 | Apparently, lovastatin's growth-inhibitory effects involve multiple mevalonate-dependent pathways that are required for allcells to growand divide. The specificity observed with L- Save | |
1993 | Campana, D;Coustan-Smith, E;Manabe, A;Buschle, M;Raimondi, SC;Behm, FG;Ashmun, R;Aricò, M;Biondi, A;Pui, CH; | Prolonged survival of B-lineage acute lymphoblastic leukemia cells is accompanied by overexpression of bcl-2 protein | Blood | 8427984 | Overexpression of bcl-2 delays the onset of apoptosis in lymphohematopoietic cells. We measured levels of bcl-2 protein in normal and leukemic human B-cell progenitors with a Save | |
1993 | Ruiz-Gómez, M;Ghysen, A; | The expression and role of a proneural gene, achaete, in the development of the larval nervous system of Drosophila | The EMBO journal | 8458326 | The late expression is abnormal in that most or allcells of the clusters shift to, and stay at, the high level of expression that is normally observed only in the progenitor cell. We conclude Save | |
1993 | Rice, NR;Ernst, MK; | In vivo control of NF-kappa B activation by I kappa B alpha | The EMBO journal | 8223478 | Whatever the basis for IxBct's instability in the cell lines examined here, there is no apriori reason to think that allcells have such unstable IxBa.For example, if the hypothetical kinase Save | |
1993 | Camplejohn, RS;Brock, A;Barnes, DM;Gillett, C;Raikundalia, B;Kreipe, H;Parwaresch, MR; | Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells | British journal of cancer | 7682430 | There is considerable interest in immunohistochemical markers of proliferation which are suitable for use on routinely fixed clinical material. The novel proliferation-associated antibody Save | |
1993 | Art, JJ;Fettiplace, R;Wu, YC; | The effects of low calcium on the voltage-dependent conductances involved in tuning of turtle hair cells | The Journal of physiology | 8308720 | Allcells tuned to very low frequencies also possessed an inward rectifier (see records in Fig. C) revealed with hyperpolarizing pulses negativeto the K+ equilibrium potential. An inward Save | |
1993 | Cociancich, S;Ghazi, A;Hetru, C;Hoffmann, JA;Letellier, L; | Insect defensin, an inducible antibacterial peptide, forms voltage-dependent channels in Micrococcus luteus | The Journal of biological chemistry | 7690029 | There are at least two possible explanations for this observation: either allcells are not hit by defensin, or they are all hit, but areonly partially depleted of K'. A partial depletion of K' Save | |
1993 | Masumoto, A;Hemler, ME; | Multiple activation states of VLA-4. Mechanistic differences between adhesion to CS1/fibronectin and to vascular cell adhesion molecule-1 | The Journal of biological chemistry | 7677996 | We examined the effects of a stimulatory anti-beta 1 mAb (TS2/16) and different divalent cations on VLA-4-mediated cell adhesion to vascular cell adhesion molecule-1 (VCAM-1), to the Save | |
1993 | Stauffer, P;Zhao, H;Luby-Phelps, K;Moss, R;Star, R;Muallem, S; | Gap junction communication modulates [Ca2+]i oscillations and enzyme secretion in pancreatic acini. | The Journal of biological chemistry | These oscillations stopped in allcells on stopping stimulationindependent of the number or the length of the focal stimulations. Global CCKJ stimulation of the same acinus also induced [ Save | ||
1993 | Sinha, SK;Todd, SC;Hedrick, JA;Speiser, CL;Lambris, JD;Tsoukas, CD; | Characterization of the EBV/C3d receptor on the human Jurkat T cell line: evidence for a novel transcript | Journal of immunology (Baltimore, Md. : 1950) | 8390533 | (28) demonstrated that HPB-ALLcells coexpress CR2 and CRl and that a portion of these two C3 receptors are associated in the form of CRl/CR2 complexes in the cell membrane. Save | |
1993 | Panzeter, PL;Ringer, DP; | DNA precursors are channelled to nuclear matrix DNA replication sites | The Biochemical journal | 8352746 | Precursors injected into the bloodstream do not have equal access to allcells, especially during the short pulse times used in this study. Many replicating cells will not see the precursor Save | |
1993 | D'Andrea, RJ;Stratmann, R;Lehner, CF;John, UP;Saint, R; | The three rows gene of Drosophila melanogaster encodes a novel protein that is required for chromosome disjunction during mitosis | Molecular biology of the cell | 8305737 | (I) An embryo during cellularization showing lack of transcripts in allcells except pole (presumptive germ) cells. 0) Cellular blastodern at higher magnification showingpunctate staining Save | |
1993 | Dorocicz, IR;Williams, PM;Redfield, RJ; | The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competence | Journal of bacteriology | 8226661 | This cAMP-independent event must be induced in allcells when cultures are transferred to MIV, but it may also occur spontaneously in the small fraction of cells that develop Save | |
1993 | Humm, J;Chin, L; | A Model of Cell Inactivation by Alpha-Particle Internal Emitters | Radiation Research | The bottom survival curve is produced by 50% of the sources bound uniformly to allcells. The middle curve is for 50% of the source decays occurring on half of the cells for type A Save | ||
1993 | Mukhtar, DD;Stewart, IJ; | Observations of the surface morphology of cultured granulated metrial gland cells | Journal of anatomy | 8226296 | The features described above were found on GMG cells at all stages of culture examined and on allcells from all stages of pregnancy examined. However, it was noticeable that the Save | |
1993 | Edelsbrunner, H;Seidel, R;Sharir, M; | On the Zone Theorem for Hyperplane Arrangements | SIAM Journal on Computing | We define Zone(b; H) to be the set of allcells in the arrangement A(H) that intersect the hypewlane b, and the cells were defined to be open sets. Previously the zone used to be defined Save | ||
1993 | Ohno, H;Inoue, T;Akasaka, T;Okumura, A;Miyanishi, S;Ohashi, I;Kikuchi, M;Masuya, M;Amano, H;Imanaka, T; | Acute lymphoblastic leukemia associated with a t(1;19)(q23;p13) in an adult | Internal medicine (Tokyo, Japan) | 8286840 | ThePCRanalysis of the present patient revealed the samemolecular evidence of E2A/PBX1 fusion as found in childhood ALLcells. Thus, molecular analysis of the E2A/PBX1fusion gene Save | |
1993 | Herrmann, H;Karsch, F; | LARGE SCALE COMPUTATIONAL PHYSICS ON MASSIVELY PARALLEL COMPUTERS | Large Scale Computational Physics on Massively Parallel Computers | time matrix vector multiplication 19.4 sec sum over allcells 0.012 sec Time for allcells to allcells communication. Save | ||
1993 | Shamim, KM; | Frog retinal ganglion cells. Morphological and spatial analyses | Thesis | For an auto-correlogram, allcells of the same type as the central reference cell are picked up (asshown schematically in A3) while a cross-correlogram locates all cellsof another type in Save | ||
1992 | Kim, CY;Giaccia, AJ;Strulovici, B;Brown, JM; | Differential expression of protein kinase C epsilon protein in lung cancer cell lines by ionising radiation | British journal of cancer | 1329908 | The effect of ionising radiation on the regulation of gene and protein expression is complex. This study focuses on the translational regulational of the epsilon isoform of protein kinase C Save | |
1992 | Rooney, IA;Davies, A;Morgan, BP; | Membrane attack complex (MAC)-mediated damage to spermatozoa: protection of the cells by the presence on their membranes of MAC inhibitory proteins | Immunology | 1374057 | On fluorescence microscopy, allcells stained brightly with both BRIC 229 and A 35 anti-CD59 antibodies (Fig. 2a-c). The pattern ofstaining vfaried (eg Fig. Save | |
1992 | Smedts, F;Ramaekers, F;Troyanovsky, S;Pruszczynski, M;Robben, H;Lane, B;Leigh, I;Plantema, F;Vooijs, P; | Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasia | The American journal of pathology | 1372156 | A completely filled bar indicates an intensely positive reaction in allcells. An open bar indicates that all cells are negative, whereas a small square indicates that only afew cells Save | |
1992 | Hohmann, HP;Remy, R;Aigner, L;Brockhaus, M;van Loon, AP; | Protein kinases negatively affect nuclear factor-kappa B activation by tumor necrosis factor-alpha at two different stages in promyelocytic HL60 cells | The Journal of biological chemistry | 1730737 | Allcells were used after reaching near confluence or a density of 1-1.5 X lo6 cells/ml of culture medium. Preparation of Nuclear Extracts and Electrophoretic Mobility Shift Assays (EMSA) Save | |
1992 | Kirby, MS;Sagara, Y;Gaa, S;Inesi, G;Lederer, WJ;Rogers, TB; | Thapsigargin inhibits contraction and Ca2+ transient in cardiac cells by specific inhibition of the sarcoplasmic reticulum Ca2+ pump | The Journal of biological chemistry | 1320000 | Recently, it has been reported that in allcells examined, a common response evoked by thapsigargin is a rapid increase in cytosolic [Ca2+],possibly resulting from the mobilization of Save | |
1992 | Bednarczyk, J;Szabo, M;McIntyre, B; | Post-translational processing of the leukocyte integrin alpha 4 beta 1. | Journal of Biological Chemistry | Endo F digestion of a4 and B1 from HPB-ALLcells. and and IL-2-dependent T lymphocytes cultured for 43 days (Fig. HPB-ALL cells were 'ZsII-cell-surface-labeled lysed in Nonidet P4). Save | ||
1992 | García-Alvarado, JS;Rodriguez, MA;Labbé, RG; | Influence of elevated temperature on starch hydrolysis by enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens type A | Applied and environmental microbiology | 1371660 | The suspension was sonicated for 45 s with 60-s cooling periods until essentially allcells were disrupted. The sample was thencentrifuged at 10,000 x g for 30 min, and Save | |
1992 | Szabà, G;Pine, PS;Weaver, JL;Kasari, M;Aszalos, A; | Epitope mapping by photobleaching fluorescence resonance energy transfer measurements using a laser scanning microscope system | Biophysical journal | 1380319 | ||
1992 | Shatz, CJ; | The developing brain | Scientific American | 26269896 | Allcells on the array fired with in about five seconds of one another, in a predictable and rhythmic pattern. The bursts of action potentials lasted several seconds and were followed by Save | |
1991 | Bejar, I; | A GENERATIVE APPROACH TO PSYCHOLOGICAL AND EDUCATIONAL MEASUREMENT | ETS Research Report Series | in this taxonomy could be assessed by the degree to which the psychometric parameters from a given cell are well-predicted and the within-cell residuais are constant across allcells. Save | ||
1991 | Burton, GF;Kosco, MH;Szakal, AK;Tew, JG; | Iccosomes and the secondary antibody response | Immunology | 1879876 | The resulting preparations were observed microscopically to ensure that allcells were disrupted. Antigen concentration was determined by using radiolabelled antigen and assessing Save | |
1991 | Hudson, TH;Grillo, FG; | Brefeldin-A enhancement of ricin A-chain immunotoxins and blockade of intact ricin, modeccin, and abrin | The Journal of biological chemistry | 1917980 | This receptor interacts with transport of newly synthesized plasma membrane glycoproproteoglycans in allcells studied the Gal bindingsitesontheB-chain of ricin and would teins, Save | |
1991 | Novy, RE;Lin, JL;Lin, JJ; | Characterization of cDNA clones encoding a human fibroblast caldesmon isoform and analysis of caldesmon expression in normal and transformed cells | The Journal of biological chemistry | 1885618 | Allcells were grownin Dulbecco's modified Eagle's mediumcontaining 10% fetal calf serum and maintained in a 37 "C humidified incubator with 95% air and 5% CO,. Western Blot and Save | |
1991 | Järveläinen, HT;Kinsella, MG;Wight, TN;Sandell, LJ; | Differential expression of small chondroitin/dermatan sulfate proteoglycans, PG-I/biglycan and PG-II/decorin, by vascular smooth muscle and endothelial cells in culture | The Journal of biological chemistry | 1744124 | ||
1991 | van Praag, MC;Boom, BW;van Hees, CL;Vermeer, BJ;Scheffer, E; | Classical and ulcerative lichen planus with plasma cell infiltrate | Archives of dermatology | 1990998 | Plasma cells accounted for 60% to 70% of allcells. The infiltrate invaded the epidermis causing apoptoticand vacuolar degeneration of basal and parabasal cells (Fig 2). The lesions of Save | |
1991 | Humbert, P;Agache, P; | Acquired ichthyosis: a new cutaneous marker of autoimmunity | Archives of dermatology | 1990997 | Plasma cells accounted for 60% to 70% of allcells. The infiltrate invaded the epidermis causing apoptoticand vacuolar degeneration of basal and parabasal cells (Fig 2). The lesions of Save | |
1991 | Hall, AJ; | Steady size distributions in cell populations : a thesis presented in partial fulfilment of the requirements for the degree of Doctor Philosophy in Mathematics at Massey University | Thesis | Special cases are also considered where allcells with the same birth-size divide at the ), and allcells divide into equal-sized daughters, then we obtain a functional differential equation. Save | ||
1990 | Finn, PW;Kara, CJ;Van, TT;Douhan, J;Boothby, MR;Glimcher, LH; | The presence of a DNA binding complex correlates with E beta class II MHC gene expression | The EMBO journal | 2328725 | Even though the three motifs do not enhance CAT activity when tested individually, allcells that we have tested contain factors that bind to W, X and Y (Figure 4). Save | |
1990 | Yayon, A;Klagsbrun, M; | Autocrine transformation by chimeric signal peptide-basic fibroblast growth factor: reversal by suramin | Proceedings of the National Academy of Sciences of the United States of America | 2164679 | Most if not allcells possess FGF receptors, which are phosphorylated andinternalized upon binding of FGF. A 130-kDa high-affinity FGF receptor containing an Save | |
1990 | Berger, JD;Rahemtullah, S; | Commitment to autogamy in Paramecium blocks mating reactivity: implications for regulation of the sexual pathway and the breeding system | Experimental cell research | 2298253 | From this point on, allcells become committed to autogamy at a cell density that is lover than that required for the onset of mating reactivity. The cell cycle experiments discussed above Save | |
1990 | Dixon, CJ;Woods, NM;Cuthbertson, KS;Cobbold, PH; | Evidence for two Ca2(+)-mobilizing purinoceptors on rat hepatocytes | The Biochemical journal | 2386488 | Other than in the total absence of receptors of both subsets, allcells will respond to ATP.We propose that the cells responding to ATP with transients of short duration lack receptors of Save | |
1989 | Malinow, R;Schulman, H;Tsien, RW; | Inhibition of postsynaptic PKC or CaMKII blocks induction but not expression of LTP | Science (New York, N.Y.) | 2549638 | Bath-applied H-7 can suppress the expression of LTP (3), although it is not known whether the locus of action is pre- or postsynaptic, since it has access to allcells in the preparation. To Save | |
1989 | Johnson, JP;Stade, BG;Holzmann, B;Schwäble, W;Riethmüller, G; | De novo expression of intercellular-adhesion molecule 1 in melanoma correlates with increased risk of metastasis | Proceedings of the National Academy of Sciences of the United States of America | 2643120 | Identical restriction fragments were observed in allcells analyzed, including four autologous melanoma/B-cell pairs. The P3.58/ICAM-1 gene, therefore, does not seem to be polymorphic Save | |
1989 | Knust, B;Brüggemann, U;Doerfler, W; | Reactivation of a methylation-silenced gene in adenovirus-transformed cells by 5-azacytidine or by E1A trans activation | Journal of virology | 2473219 | Of course, it was not possible to show that allcells had been transfected equally efficiently. Reactivation of E2Atranscription might have occurred only in a subpopulation of the HE3 cells. Save | |
1989 | Celis, E;Karr, RW; | Presentation of an immunodominant T-cell epitope of hepatitis B surface antigen by the HLA-DPw4 molecule | Journal of virology | 2463381 | Thus, it appears that in the heterogeneous PBMC population not allcells express DPw4 molecules and that only thosethat do can present HBsAg (or Sld) to the Th. The inhibitory effect Save | |
1989 | Navaratnam, V;Woodward, JM;Skepper, JN; | Specific heart granules and natriuretic peptide in the developing myocardium of fetal and neonatal rats and hamsters | Journal of anatomy | 2532637 | Within the fetal atria, almost allcells seem capable of ANP synthesis and the concentration of ANP immunoreactivity increases sharply with development even after birth. Recent light Save | |
1988 | Nicolson, GL;Dulski, KM;Trosko, JE; | Loss of intercellular junctional communication correlates with metastatic potential in mammary adenocarcinoma cells | Proceedings of the National Academy of Sciences of the United States of America | 3422438 | , and the gene coding for polyoma virus middlesized tumor (middle T) antigen can decrease intercellular junctional communication in certain cellsystems (13-16), although not allcells Save | |
1988 | Hall, JG; | Review and hypotheses: somatic mosaicism: observations related to clinical genetics | American journal of human genetics | 3052049 | Conditions such as McCune-Albright syndrome may well represent lethal human mutations when present in allcells, but, by isolating the tissue involved, it may be possible to compare Save | |
1988 | Siebenlist, U;Bressler, P;Kelly, K; | Two distinct mechanisms of transcriptional control operate on c-myc during differentiation of HL60 cells | Molecular and cellular biology | 3162550 | These sites occur in allcells which we have analyzed (46, 47). In previous reports (3, 20, 22, 28, 29), only partial and even somewhat conflicting information on the chromatin structure of Save | |
1988 | Adány, R;Glukhova, MA;Kabakov, AY;Muszbek, L; | Characterisation of connective tissue cells containing factor XIII subunit a | Journal of clinical pathology | 2893813 | Though the expression of certain antigens might be lost at culture, allcells stained for the fibroblast markers IIIG1O and alkaline phosphatase lack this antigen. This clearly suggests that Save | |
1988 | Kim, T;Zeigler, B; | The class kernel-models in DEVS-scheme: a hypercube architecture example | ACM SIGSIM Simulation Digest | set-allcells-positions, which generates 2 n number of n-tuple coordinates to be attached to corresponding components before locating them in their positions. The method set-allcells- Save | ||
1988 | Reno, PW; | Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions | Coastal and Estuarine | N ear ly identic al reactions were found in kidney (Figure 2?) and liver (data not shown) -allcells seeme d to conta in IPN antige n. However, gastrointestin al tra ct tissue, especiallyt he Save | ||
1987 | Art, JJ;Fettiplace, R; | Variation of membrane properties in hair cells isolated from the turtle cochlea | The Journal of physiology | 2443666 | The shape of the current-voltage relationship was found to be similar in allcells, being flat at hyperpolarized levels and increasing its slope with depolarization through the resting Save | |
1987 | Segarini, PR;Roberts, AB;Rosen, DM;Seyedin, SM; | Membrane binding characteristics of two forms of transforming growth factor-beta | The Journal of biological chemistry | 2889729 | Essentially allcells have high affinity receptors that specifically bind TGF-/3 (Kdvalues of 1-60 pM, Refs. 11, 12, 14, and 15). The receptor number is tissue- and species-specific and Save | |
1987 | Richardson, A;Butler, JA;Rutherford, MS;Semsei, I;Gu, MZ;Fernandes, G;Chiang, WH; | Effect of age and dietary restriction on the expression of alpha 2u-globulin | The Journal of biological chemistry | 2442168 | regulation of gene expression represents one site regulation of common to allcells in a living organism, and changes in gene 24. expression can have profound effect at both the a Save | |
1987 | ROBERTS, D; | A zonal method for modeling 3-D aircraft flow fields with jet plume effects | 19th AIAA, Fluid Dynamics, Plasma Dynamics, and Lasers Conference | CON is then updated, and the cycle 18 repeated until the error in continuity falls below a prescribed tolerance for allcells . The relaxation factor, 0, Save | ||
1986 | Greaves, MF; | Differentiation-linked leukemogenesis in lymphocytes | Science (New York, N.Y.) | 3535067 | us the monodonal antibody-defined makers T9 and T10, described initially on T-ALLcells and normal thymocyte subses (21), are not T lineage or maturation stage restrictedbut are Save | |
1986 | Mann, GJ;Fox, RM; | Deoxyadenosine triphosphate as a mediator of deoxyguanosine toxicity in cultured T lymphoblasts | The Journal of clinical investigation | 3490493 | Allcells were maintained at 37C in suspension culture in Hepes-buffered Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% fetal bovine serum, glutamine (2 Save | |
1986 | Slater, LM;Sweet, P;Stupecky, M;Gupta, S; | Cyclosporin A reverses vincristine and daunorubicin resistance in acute lymphatic leukemia in vitro | The Journal of clinical investigation | 3457021 | It has recently been reported that human ALLcells have a 10-fold greater concentration of calmodulin than normal peripheral blood lymphocytes and that CsA is a potentinhibitor of T Save | |
1986 | Cowley, GP;Smith, JA;Gusterson, BA; | Increased EGF receptors on human squamous carcinoma cell lines | British journal of cancer | 2420349 | The binding kinetics for allcells examined showed the typical pattern of a high and low affinity receptor with dissociation constants of 10-10M and 1-9M respectively. Recent Save | |
1986 | Stewart, MA;Warnock, M;Wheeler, A;Wilkie, N;Mullins, JI;Onions, DE;Neil, JC; | Nucleotide sequences of a feline leukemia virus subgroup A envelope gene and long terminal repeat and evidence for the recombinational origin of subgroup B viruses | Journal of virology | 3009890 | FEA cells (21) were infected in mass culture with various FeLV isolates and passaged for 3 weeks to allow virus spread to allcells. DNA was purified from these cell cultures for use in Save | |
1986 | DiFrancesco, D;Ferroni, A;Mazzanti, M;Tromba, C; | Properties of the hyperpolarizing-activated current (if) in cells isolated from the rabbit sino-atrial node | The Journal of physiology | 2432247 | The reason for this discrepancy may be the current run-down that occurs, although to a highly variable degree, in allcells. This feature of if is treated insome detail below. Here it may be Save | |
1986 | Hartman, BJ;Tomasz, A; | Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureus | Antimicrobial agents and chemotherapy | 3488015 | explanation of heterogeneity is that allcells in the heterogeneous culture carry the genetic marker(s) of methicillin resistance, but for some reason the phenotypic expression of Save | |
1986 | Wang, CY;Bushkin, Y;Pica, R;Lane, C;McGrath, H;Posnett, DN; | Stimulation and expansion of a human T-cell subpopulation by a monoclonal antibody to T-cell receptor molecule | Hybridoma | 2429909 | Both the aand (3-chains from normal PBL-T blasts resolved in the pi range of 5.5-6.5, whereas the a- and /3-chains from HPB-ALLcells had acidic and basic pis of 4.0 and 7.0-8.5, Save | |
1986 | Steiness, E; | Positive inotropic drugs--digitalis | Acta medica Scandinavica. Supplementum | 3017057 | Digitalis is known to inhibit the Na+K+-ATPase in allcells in nearly all species. It has been aqyed that this effect induces increase of the inuacellular sodium concentnfion within the Save | |
1985 | Lehrer, RI;Daher, K;Ganz, T;Selsted, ME; | Direct inactivation of viruses by MCP-1 and MCP-2, natural peptide antibiotics from rabbit leukocytes | Journal of virology | 2985808 | Allcells used in this manner were obtained from the American Type Culture Collection except for MDBK cells, which were a gift from Debi Nayak. The virus-infected cell monolayers Save | |
1985 | Morris, CS;Hitchcock, E; | Immunocytochemistry of folliculo-stellate cells of normal and neoplastic human pituitary gland | Journal of clinical pathology | 3889065 | folliculo-stellate cells stain for GFAP and S100, although not allcells stained for S100 are GFAP positive. Cocchia and Miani" showed S100 protein within the stellate cells of the pars Save | |
1985 | Neyses, L;Locher, R;Stimpel, M;Streuli, R;Vetter, W; | Stereospecific modulation of the calcium channel in human erythrocytes by cholesterol and its oxidized derivatives | The Biochemical journal | 2581557 | Furthermore, allcells were energized to the same extent by ATP (about 1.7mM). Since the anion channel may be influenced by cholesterol (Schubert & Boss, 1982), we checked Save | |
1985 | Hueston, WD; | Herd summaries of individual cow somatic cell counts and mastitis control research | Thesis | and th e re fo re abnormal; 2) allcells in milk were desquamated se c re to ry cells of epithelialo rig in , and th e re fo re a normal p art of la c to genes is ; and 3) the cells in normal milk Save | ||
1984 | Buick, RN;Pollak, MN; | Perspectives on clonogenic tumor cells, stem cells, and oncogenes | Cancer research | 6386145 | In prokaryotes, control of proliferation is unrelated to the process of differentiation, and allcells have proliferative potential. As we have shown, this is not the case in tissues of higher Save | |
1984 | Gaukroger, JM;Wilson, L; | Protection of cells from methotrexate toxicity by 7-hydroxymethotrexate | British journal of cancer | 6590087 | Cell growth survival studies have revealed that 7-OH methotrexate is two orders of magnitude less cytotoxic to human melanoma and human acute lymphoblastic leukaemia (ALL) cells Save | |
1984 | Gussander, E;Adams, A; | Electron microscopic evidence for replication of circular Epstein-Barr virus genomes in latently infected Raji cells | Journal of virology | 6092676 | Circular forms, which can be isolated either in the covalently closed, supercoiled structure or as a relaxed, open DNA circle (16), have subsequently been found in allcells with more Save | |
1984 | Epstein, LM;Forney, JD; | Mendelian and non-mendelian mutations affecting surface antigen expression in Paramecium tetraurelia | Molecular and cellular biology | 6092921 | A genetic and molecular analysis of this mutant suggests that although the A gene is present in the micronucleus of allcells, it is lost during the formation of the macronucleus in the Save | |
1984 | Yinon, U; | Myopia induction in animals following alteration of the visual input during development: a review | Current eye research | 6713963 | For example, asfar as has been determined, allcells of the salamander are capable of ded if fe rent iat ion with appropriate stimulation. Extensive in vitro experimentation on amphibian Save | |
1984 | Nelson, DO;Graham, CA; | Effects of development and captopril treatment on central angiotensin II--sensitive neurons | Clinical and experimental hypertension. Part A, Theory and practice | 6398145 | Nearly allcells examined, including neurons which were relatively insensitive to A11 or saralasin, were potently ex | |
1983 | Overath, P;Czichos, J;Stock, U;Nonnengaesser, C; | Repression of glycoprotein synthesis and release of surface coat during transformation of Trypanosoma brucei | The EMBO journal | 6688990 | Six hours after transfer of the cells to medium SDM-79, ie, at a time when VSG synthesis has already ceased, allcells retain the coat (compare sample at t= 6 h with freshly harvested Save | |
1983 | Rowin, KS;Tanowitz, HB;Wittner, M;Nguyen, HT;Nadal-Ginard, B; | Inhibition of muscle differentiation by trypanosoma cruzi | Proceedings of the National Academy of Sciences of the United States of America | 6413976 | Normal quantities of 13 and y-actin were present in allcells. (B) Rehybridization of the RNA blot shown in A with 32P-labeled DNA withnonmuscle-specific sequencescoding Save | |
1983 | Meo, T;Gramsch, C;Inan, R;Höllt, V;Weber, E;Herz, A;Riethmüller, G; | Monoclonal antibody to the message sequence Tyr-Gly-Gly-Phe of opioid peptides exhibits the specificity requirements of mammalian opioid receptors | Proceedings of the National Academy of Sciences of the United States of America | 6191329 | Allcells in the intermediate lobe of rat pituitary were intensely stained (Fig. 3). Higher magnification revealed heavy cytoplasmic localization of the immunoreactive peptides. Save | |
1983 | Otteskog, P;Sundqvist, KG; | Anchorage and lymphocyte function. Spreading-capacity distinguishes common thymocytes and peripheral T lymphocytes | Immunology | 6339376 | T-enriched human blood lymphocytes with an adhesive surface in the presence of Concanavalin A (Con A) almost immediately induced a sequence of motilechanges in virtually allcells Save | |
1983 | O'NEIL, PM; | Log-linear models for evaluating hunting demand | Thesis | Both the Pearson ch i-sq ua re statistic and th e lik e lih oodratio sum over allcells in th e ta b le . Both a re asymp to tic a lly d is tribu te d as chi - square . Save | ||
1983 | Burman, LG;Raichler, J;Park, JT; | Evidence for diffuse growth of the cylindrical portion of the Escherichia coli murein sacculus | Journal of bacteriology | 6350274 | The lengths of allcells that contained grains and the distances of the grains from the nearest cell pole were measured with a ruler. Forplotting, these distances were expressed as Save | |
1982 | Marx, JL; | Cloning the genes of the MHC | Science (New York, N.Y.) | 7071587 | These regions code not for transplantation antigens, which are expressed on allcells, but for differentiation antigens that are foundonly on certain types of immune cells. The differ Save | |
1982 | Zingg, HH;Patel, YC; | Biosynthesis of immunoreactive somatostatin by hypothalamic neurons in culture | The Journal of clinical investigation | 7130395 | Immediately after being plated, allcells exhibited a uniform round-shaped appearance and were evenly distributed on the plate surface. After day 5 in culture, many cells showed a Save | |
1982 | Donta, ST;Sullivan, N;Wilkins, TD; | Differential effects of Clostridium difficile toxins on tissue-cultured cells | Journal of clinical microbiology | 7107845 | The two toxins' effects are not similar on allcells, however, since they produce different Allcells were tested at the same time with identical toxin preparations. b ++, All cells rounded Save | |
1982 | Ueda, R;Tanimoto, M;Takahashi, T;Ogata, S;Nishida, K;Namikawa, R;Nishizuka, Y;Ota, K; | Serological analysis of cell surface antigens of null cell acute lymphocytic leukemia by mouse monoclonal antibodies | Proceedings of the National Academy of Sciences of the United States of America | 6956869 | Production of Hybridomas Against063 Null-ALLCells. ALL cells wereobtained fromtheperipheralblood ofa22-yearold female patient. The surface phenotypeof the leukemia cells Save | |
1982 | Cochrane, DE;Distel, DL; | Association of 45calcium with rat mast cells stimulated by 48/80: effects of inactivation, calcium and metabolic inhibition | The Journal of physiology | 6816921 | Mast cells were stimulated by48/80 (1 4g/ml.) and unlabelled Ca (1 mM) added to allcells 10 sec later. Atthe indicated times afterward, 4"Ca (50 #sC/ml.)was added and the incubation Save | |
1982 | Ayres, K;Sklar, R;Larson, K;Lindgren, V;Strauss, B; | Regulation of the capacity for O6-methylguanine removal from DNA in human lymphoblastoid cells studied by cell hybridization | Molecular and cellular biology | 6897101 | so that the dose shown is that which would be observed if allcells had the reactivity of ROT-5. Symbols: A, ROT-5; 0, L6; O, hybrid clone no. 2; E, hybrid clone no. Save | |
1982 | Little, LM;Shadduck, JA; | Pathogenesis of rotavirus infection in mice | Infection and immunity | 6292110 | It should be appreciated that in the current study not allcells within any anatomical region fluoresced. Rather, fluorescing cells normally occupied less than 10%, up to perhaps 50%, of Save | |
1982 | 山田和義, ;岡部直衛, ;宇塚善郎, ; | FAB 分類における成人急性白血病の走査電顕及び表面マーカーによる解析 | 臨床血液 | Almost allcells have ridge-like pro丘1es.(×5,800) Almost allcells are positive for both ANE and CAEin the same cell. b:Almost allcells have ridge-1ike pro丘Ies or microvilli・(×3,000) C: Save | ||
1981 | Abbott, A; | ¡Graphs, Not Punctuation! | Science | Indeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save | ||
1981 | Libet, B; | " Human Life" Testimony | Science | 7244627 | Indeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save | |
1981 | Osborn, EF; | Hubbert's Estimates | Science (New York, N.Y.) | 17782756 | Indeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save | |
1981 | Hamp, EP; | ;Graphs, not punctuation! | Science (New York, N.Y.) | 17782755 | Indeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save | |
1981 | Engleman, EG;Warnke, R;Fox, RI;Dilley, J;Benike, CJ;Levy, R; | Studies of a human T lymphocyte antigen recognized by a monoclonal antibody | Proceedings of the National Academy of Sciences of the United States of America | 7015346 | A second screening identified a subgroup of these clones whose antibody products bound equally to normal peripheralTcells andto the immunizing T-ALLcells. The binding specificity of Save | |
1981 | Yokoyama, K;Stockert, E;Old, LJ;Nathenson, SG; | Structural comparisons of TL antigens derived from normal and leukemia cells of Tl+ and TL- strains and relationship to genetically linked H-2 major histocompatibility complex products | Proceedings of the National Academy of Sciences of the United States of America | 6947273 | H-2 antigens are expressedon virtually allcells ofthe body, whereas TL antigensare normally restrictedto thymocytes in TL+ strains. In contrast to the remarkable polymorphism of H-2 (10 Save | |
1981 | Hanania, N;Shaool, D;Poncy, C;Harel, J; | Manifold expression of new cellular genes in human lymphoid neoplasia | Proceedings of the National Academy of Sciences of the United States of America | 6947241 | Previous studies performed in our laboratory have shown that a minor single-stranded DNA component (ssDNA) isolated from the bulk of double-stranded nuclear DNA (nDNA) of Save | |
1981 | Mechler, B;Rabbitts, TH; | Membrane-bound ribosomes of myeloma cells. IV. mRNA complexity of free and membrane-bound polysomes | The Journal of cell biology | 6782109 | Allcells were harvested during exponentialgrowth from cultures not exceeding 5.0 x 106 cells/ml. When labeling was required, [ 3H]uridine, [3Madenosine, or [ 3H]oleic acid wasadded Save | |
1981 | Knipe, DM;Batterson, W;Nosal, C;Roizman, B;Buchan, A; | Molecular genetics of herpes simplex virus. VI. Characterization of a temperature-sensitive mutant defective in the expression of all early viral gene products | Journal of virology | 6264126 | However, the yield of syn+ virus was approximately 10% of the yield of the syn virus, even though allcells were coinfected with both. Therefore, even the wild-type parent of tsB7 could Save | |
1981 | Grover, NB;Margalit, A;Zaritsky, A;Ben-Hur, E;Hansen, MT; | Sensitivity of exponentially growing populations of Escherichia coli to photo-induced psoralen-DNA interstrand crosslinks | Biophysical journal | 7023563 | ||
1981 | Zaritsky, A;Macnab, RM; | Effects of lipophilic cations on motility and other physiological properties of Bacillus subtilis | Journal of bacteriology | 6792185 | The effects of TPAs+ on the motor were readily reversible: 20 min after treatment with 50 ,uM TPAs+, cells were filtered, washed, and suspended in chemotaxis buffer; 3 minlater, allcells Save | |
1981 | Lindgren, V; | The location of chromosome breaks in Bloom's syndrome | Cytogenetics and cell genetics | 7471822 | Average of samples IB and 2 only; when allcells are included, the average is 50.7%. Table II. Distribution of breaks in late-non-late-replicating areas1 Total Save | |
1981 | Carpio, M;Bellamy, JE;Babiuk, LA; | Comparative virulence of different bovine rotavirus isolates | Canadian journal of comparative medicine : Revue canadienne de medecine comparee | 6268264 | Twenty-four hours postinfection, when allcells were detached from the monolayers, the entire culture fluids were sonicated to release intracellular virus. The virus was pelleted through a Save | |
1981 | Thaller, RA; | Potential use of 52FE porphyrins as tumor scanning agents | Thesis | However in vivo tumor killing was not complete resulting in many smaller tumors due to the factthat not allcells were exposed to the light or took up the hematoporphyrin. (220) Over 300 Save | ||
1980 | O'Lague, PH;Huttner, SL; | Physiological and morphological studies of rat pheochromocytoma cells (PC12) chemically fused and grown in culture | Proceedings of the National Academy of Sciences of the United States of America | 6929516 | In allcells regardless of growth conditions membrane resistance decreased with depolarization. Fig. 1C shows superimposed voltage responses to one depolarizing and one hyperpo Save | |
1980 | Lasky, LA;Lev, Z;Xin, JH;Britten, RJ;Davidson, EH; | Messenger RNA prevalence in sea urchin embryos measured with cloned cDNAs | Proceedings of the National Academy of Sciences of the United States of America | 6254074 | per average cell given on the lower abscissas are minimal estimates calculated on the basis that there are 600 cells per gastrula and 1500 cells per pluteus embryo and that allcells are Save | |
1980 | Haas, M;Patch, V; | Genomic masking and rescue of dual-tropic murine leukemia viruses: role of pseudotype virions in viral lymphomagenesis | Journal of virology | 6252327 | Allcells were grown in Dulbecco-Vogt modified Eagle minimum essential medium supplemented with 10%o fetal bovine serum. All cells were split routinelyat a ratio of 1:16 upon Save | |
1980 | Weller, SK;Joy, AE;Temin, HM; | Correlation between cell killing and massive second-round superinfection by members of some subgroups of avian leukosis virus | Journal of virology | 6245246 | this study were determined by interference assays as follows: chicken cells in 100-mm petri dishes were infected with different avian leukosis viruses and passaged twice so that allcells Save | |
1980 | Berg, JM;Gardner, HA;Gardner, RJ;Goh, EG;Markovic, VD;Simpson, NE;Worton, RG; | Dic(21;21) in a Down's syndrome child with an unusual chromosome 9 variant in the mother | Journal of medical genetics | 6445984 | In allcells analysed, the translocation chromosome was of the same relative size and banding pattern, indicating a high degree of stability. No short structures or chromosome fragments Save | |
1980 | Gerrard, JM;Clawson, CC;White, JG; | Lysophosphatidic acids: III. Enhancement of neutrophil chemotaxis | The American journal of pathology | 7416233 | Figure 2-When 0.35 ,ug FMP/ml was added to the incubation media used in Figure 1, marked changes were seen in allcells. There were large projections from the body of the cell, and Save | |
1980 | Brown, AT;Breeding, LC; | Carbon dioxide metabolism by Actinomyces viscosus: pathways for succinate and aspartate production | Infection and immunity | 6769822 | Allcells were washed once with 0.01 M potassium phosphate buffer, pH 7.0, and the cell pellets were frozen at -20'C until used. Laboratory cultures of A. viscosus were stored as Save | |
1980 | Koppes, LJ;Nanninga, N; | Positive correlation between size at initiation of chromosome replication in Escherichia coli and size at initiation of cell constriction | Journal of bacteriology | 6995452 | We have also assumed equal growth rate constants for allcells. Variation of the growth rate will tend to decrease the correlation. However, the CV of the specific growth rate is probably Save | |
1980 | Ho, CC; | Interaction studies of ethanol and psychotropic drugs in rodents: possible involvement of adenosine triphosphatases | Thesis | D evelopm ent of th e Sodium Pump C oncept T here is ad iffe re nce in io n ic concen tra tio n betw een intracellular and ex tr ace llu la rf lu id s in allcells . Save | ||
1979 | Detwiler, PB;Hodgkin, AL; | Electrical coupling between cones in turtle retina | The Journal of physiology | 225478 | If it is assumed that allcells have the same total membrane resistance, rm, but that different pairs differ in the amount of coupling, then this method of plotting should bring all the points Save | |
1978 | Mukherjee, AB;Orloff, S;Butler, JD;Triche, T;Lalley, P;Schulman, JD; | Entrapment of metaphase chromosomes into phospholipid vesicles (lipochromosomes): carrier potential in gene transfer | Proceedings of the National Academy of Sciences of the United States of America | 349565 | Allcells were checked for mycoplasma (10) and foundto be free of those pathogens. These hybrid cells contributed the metaphase chromosomes which were used to prepare the Save | |
1978 | Esber, EC;Movassaghi, N;Leikin, SL; | Surface membrane determinants on childhood acute lymphocytic leukemia cells: immunoglobulin, Fc and C3 receptors | Clinical and experimental immunology | 278686 | Surface membrane determinants on ALLcells Other investigators have found Fc receptors on ALLcells, in combination with either E-rosette formation or with C3 receptors (Haegert et al., Save | |
1977 | Thoene, JG;Oshima, RG;Ritchie, DG;Schneider, JA; | Cystinotic fibroblasts accumulate cystine from intracellular protein degradation | Proceedings of the National Academy of Sciences of the United States of America | 270698 | After 20 hr, allcells were depleted of intracellular cystine bytreatment with MEA for 1 hr,and then placed in cystine-deficientmedium. Duplicate plates were harvested-at the times shown Save | |
1977 | Basset, F;Soler, P;Jaurand, MC;Bignon, J; | Ultrastructural examination of broncho-alveolar lavage for diagnosis of pulmonary histiocytosis X: Preliminary report on 4 cases | Thorax | 302040 | and 2) in great numbers in case t, amounting to 7% of allcells examined. In the three other patients, HX cells were much less numerous, but their presence was still diagnostic. In all four Save | |
1977 | Genther, CS;Schoeny, RS;Loper, JC;Smith, CC; | Mutagenic studies of folic acid antagonists | Antimicrobial agents and chemotherapy | 329758 | Since the folic acid pathway is common to allcells, the mechanisms operative with S. faecium will probably be similar in other types of organismssusceptible to these compounds. When Save | |
1977 | Rubenstein, M; | The effect of salicylate on macromolecular synthesis in mammalian and bacterial cells. | Thesis | The inhibition obtained in the growth experiment applied to allcells involved and did not select for a small population of cellswhile drastically inhibiting the remainder. Save | ||
1977 | Tso-Kai Liu, ; | Synthesis of Multilevel Feed-Forward MOS Networks | IEEE Transactions on Computers | Theorem 1: There always exists a feed-forward MOS network with a minimum number ofcells, realizing a given function f such that the output functions of allcells in the network are Save | ||
1976 | Bootello, A;Fernandez-Cruz, E;Escartin, P;Blanco, MF;Gosalvez, M;Segovia De Arana, JM; | Injurious effect on rat liver mitochondria by lymphocytes from patients with primary biliary cirrhosis | Clinical and experimental immunology | 1277585 | The antibody is non-organ/non-species-specific and reacts with allcells containing mitochondria. The antigen is a lipoprotein localized in the inner mitochondrial membranes (Berg et al. Save | |
1976 | Gilligan, T;Patterson, A; | High field conductance of some acids, bases, and salts | The Journal of Physical Chemistry | Particular care was taken to determine and follow the cell constants of allcells by the method of Jones and Bradshaw.11 It has been found that the cell constants of cells made with Save | ||
1976 | Malek, KW; | A METHOD FOR THE CULTIVATION OF DISPERSED EPITHELIAL CELLS FROM MOUSE PALATAL MUCOSA AND THE EFFECTS OF X-IRRADIATION. | Thesis | lted in the release of cells from the ba sa l layer and a ls o from the lower layers of the stratum spinosum, whereas 30 minutes of incubation resulted in the d isp er sio n of almost allcells Save | ||
1975 | Hyde, GM; | MILK YIELD AS A FACTOR IN DAIRY HERD MANAGEMENT BY EXCEPTION. | Thesis | Table 4 gives regression coefficients from the analyses over allcells, by parity, by age group, and by season of calving. Calculatedpeak times of lactation are also tabulated. Note that Save | ||
1975 | McKEE, RH; | THE CONTRIBUTION OF SINGLE-STRAND BREAKS AND ALKALI-LABILE BONDS TO THE LOSS OF INFECTIVITY OF GAMMA-IRRADIATED PHI-X174 REPLICATIVE FORM DNA IN E. COLI CELLS MUTANT IN VARIOUS REPAIR FUNCTIONS | Thesis | Twenty-one radiation sensitive mutants have been examined for their capacity to support y-irradiated 0X174 RF-DNA. The survival of 0X174 RF-DNA was reduced in essentially all of Save | ||
1975 | Fiske, RA;Klein, PA; | Effect of immunosuppression on the genetic resistance of A2G mice to neurovirulent influenza virus | Infection and immunity | 163800 | This putative replication defect, however, must be highly selective, ie, must occur in some but not allcells of the A2G mouse, since WSN can undergo complete cycles of replication in Save | |
1975 | Goldhammer, L; | ATS-6 Solar Cell Flight Experiment After 325 Days in Synchronous Orbit | IEEE Transactions on Aerospace and Electronic Systems | Allcells performed well through 325 days in orbit, except the FEP-covered cells, which appear to have increased their rate of degradation during the first eclipse season. Save | ||
1975 | Schriber, S; | Comparison of standing wave accelerators operating in the 2π/3 and π/2 modes | IEEE Transactions on Nuclear Science | The singly periodic (allcells identical) 23 cell system used for experimental measurements of relative axial electric field amplitudes hadthe following mean parameters; Save | ||
1974 | Baker, MA;Ramachandar, K;Taub, RN; | Specificity of heteroantisera to human acute leukemia-associated antigens | The Journal of clinical investigation | 4140196 | Similarly, antisera to ALLcells absorbed with AML cells retained significant cytotoxicity only to ALL cells. Control antisera raised in nontolerant mice were cytotoxic to all cells tested. Save | |
1974 | Rutishauser, U;Yahara, I;Edelman, GM; | Morphology, motility, and surface behavior of lymphocytes bound to nylon fibers | Proceedings of the National Academy of Sciences of the United States of America | 4545428 | Allcells were firmly attached to the fibers, did not detach even after incubation for 4 hr, and in no case was translocation of a cell along the fiber observed. A small number of cells nonspe Save | |
1974 | Stella, JP;Yankaskas, KD;Morgan, JH;Fox, MP;Pfau, CJ; | Characteristics of the in vitro inhibition of arenavirus synthesis by bis-benzimidazoles | Antimicrobial agents and chemotherapy | 4451346 | After an additional 24 h, allcells appeared to have normal morphologies, but the reticule counts from drug-treated cultures were 10% below those of the controls. Moreover, after Save | |
1974 | VAIL, AF; | DROPOUT FROM PSYCHOTHERAPY AS RELATED TO PATIENT-THERAPIST DISCREPANCIES,THERAPIST CHARACTERISTICS, AND INTERACTION IN RACE AND SEX | Thesis | This could be co rre c te dfor by tran sfo rm in g th e num ber of rem ainers in allcells , as ifallcells had the same total of dropouts and rem ainers. I t was arbitrarily decided to treatall the Save | ||
1974 | Maccallum, RC; | A COMPARISON OF TWO INDIVIDUAL DIFFERENCES MODELS FOR MULTIDIMENSIONAL SCALING: CARROLL AND CHANG'S INDSCAL AND TUCKER'S THREE-MODE FACTOR ANALYSIS | Thesis | Due to high costs of generating replications of data sets to fit thisdesign, and then analyzing the data, it was not possible to represent allcells of Table 3 in the present study. Only the Save | ||
1974 | Johnson, BS; | IMITATION BY CHILDREN OF MODEL-PERFORMED BEHAVIOR UNDER A VARIETY OF STIMULUS CONDITIONS. | Thesis | A test of the equality of regression within allcells was performed, and the hypothesis of differentialwithin cell regression was not supported (F = .62; Save | ||
1974 | VON BERGEN JR, CW; | Individual differences variables and performance goals as determinants of level of performance. | Thesis | To accomplish this a procedure was employed which essentially involved equating the n Ach scores for allcells ofthe self-esteem analysisandthe locusof Save | ||
1974 | Burnstein, T;Jacobsen, LB;Zeman, W;Chen, TT; | Persistent infection of BSC-1 cells by defective measles virus derived from subacute sclerosing panencephalitis | Infection and immunity | 4215764 | Fluorescent measles antigen was present in allcells. Co-cultures of IP-3-Ca cells with normal BSC-1 cells developed massive syncytia and extensive destruction. The IP-3-Ca cells Save | |
1974 | Katsura, Y;Uesaka, I; | Assessment of germ tube dispersion activity of serum from experimental candidiasis: a new procedure for serodiagnosis | Infection and immunity | 4207514 | Allcells in the suspension were yeast form, with a few paired, but no germ tubes or pseudohyphae were found. Intraperitoneal (ip) infection was made by inoculating 0.5 ml of the Save | |
1974 | Heilmann, LJ; | Physical studies of unfolded 30S ribosomal subunits of Escherichia coli | Thesis | E ar ly work establishedthat th eseparticles were ap parentlyub iq u it ous in allcells and consisted of ap p ro x imately eq ualproportions of RNA and protein. E ar ly pre parations# called Save | ||
1974 | Bendix, ME; | A study of the adenyl cyclase activity in testis of maturing chinook salmon (Oncorhynchus tshawytscha) | Thesis | Within each cyst allcells are at the same stage of spermatogenic development and are maturing at the same rate but within a testis the various cysts may be at different stages of Save | ||
1973 | Warburton, D;Henderson, AS;Shapiro, LR;Hsu, LY; | A stable human dicentric chromosome, t dic (12;14)(p13;p13) including an intercalary satellite region between centromeres | American journal of human genetics | 4716663 | Allcells from a peripheral blood culture had 45 chromosomes, with a missing D and C group chromosome and an abnormal large submetacentric chromosome with a prominent Save | |
1973 | BERNS, A;AYAD, S; | Intergeneric Transformation in Bacillus subtilis | Biochemical Society Transactions | allcells may be potentialrecipientsof fore ig n MA? as a consequence of th euniversalityof th egenet ic code - on thisplanetatleast*In many ca se s? up ta ke and " in tegration " offore ig n Save | ||
1973 | Dadds, MJS; | Detection and survival of Zymomonas in breweries | Thesis | Methods were developed for detecting zymomonads in beer and brewing materials to and the microbiological quality control of beer and to investigate the incidence and survival of Save | ||
1972 | Eaton, DC; | Potassium ion accumulation near a pace-making cell of Aplysia | The Journal of physiology | 5071402 | If it is assumed that the real capacitance of a small unit area of membrane is the same for allcells in Aplysia, then capacitances larger than expected represent an underestimation of the Save | |
1972 | Munthe, E;Natvig, JB; | Immunglobulin classes, subclasses and complexes of IgG rheumatoid factor in rheumatoid plasma cells | Clinical and experimental immunology | 4563335 | Allcells binding RB200 conjugate fixed also the FITC conjugate in the second step because of binding either to the plasma cell rheumatoid factor Ig or to the added RB200-labelled Save | |
1972 | Sander, G;Jacobs, JP; | Observations on interactions between fluorescein-iso-thiocyanate conjugates and cells in vitro | Journal of clinical pathology | 4114699 | At each passage allcells were treated in an identical manner. The methods employed were as described in a previous communication by Sander (1969) and Sander et al (1970). The Save | |
1972 | Penn, RF; | An experimental study involving the use of contract evaluation in a chem study and in a traditional high school chemistry course | Thesis | TEST OF EQUALITY OFREGRESSION IN ALLCELLS Save | ||
1972 | Robertson, A;Cohen, MH; | Control of developing fields | Annual review of biophysics and bioengineering | 4405416 | Wolpert's definition, "a field is that region in which allcells are having their positional information specified with respect to the same set of points," is the most useful one for our purposes, Save | |
1971 | Blankley, WF; | Auxotrophic and heterotrophic growth and calcification in coccolithophorids | Thesis | Allcells retained a complete coccolith cover throughout the experiment(see also Fig. IV.?). Allcells retained a complete coccolith cover throughout the experiment. Save | ||
1971 | BIEGEN, DA; | The effects of irrelevant and immaterial data on problem difficulty | Thesis | Id ea lly , th is 'coefficient ' would be app lica b le w ith in allcells . Save | ||
1971 | Jacobs, NJ;Jacobs, JM;Brent, P; | Characterization of the late steps of microbial heme synthesis: conversion of coproporphyrinogen to protoporphyrin | Journal of bacteriology | 4935319 | similar in allcells, this reaction should be demonstrable in all heme-containing bacteria. In addition, we also partially characterized the enzyme system in E. coli, which converts copropor Save | |
1970 | Brooks, JE; | Disuse atrophy of muscle. Intracellular electromyography | Archives of neurology | 5409598 | The amount of depolarization necessary to induce an action potential was measured in allcells stimulated. The critical depolari¬ zation (CD) in normal fibers ranged from 6 mv to 36 mv Save | |
1970 | Jayaraman, S; | Radiation Damage and Repair After Wholebody Or Partial-body Irradiations of Tenebrio Molitor Larvae | Thesis | Because during the larval metamorphosis, not allcells divide simultaneously and because their growth is discon¬tinuous, it is conceivable that exposures that do not cause thedeath of Save | ||
1970 | Mehall, AG; | Capacity of anterior pituitary grafts to correct modified growth and development of selected long bones of hypophysectomized chick embryos | Thesis | 3 no morphological differentiation in limbbud; shapes of bones pre-determlned; allcells of bud equivalent and may formcartilage or muscle up to stage 22 Save | ||
1970 | Jones, JM;Schwab, JH; | Effects of streptococcal cell wall fragments on phagocytosis and tissue culture cells | Infection and immunity | 16557722 | Allcells containing one or more bacteria were counted. No correction was attempted for bacteriawhich may have only settled on top of phagocytic cells and were not taken up, since it Save | |
1969 | McClure, HM;Belden, KH;Pieper, WA;Jacobson, CB; | Autosomal trisomy in a chimpanzee: resemblance to Down's syndrome | Science (New York, N.Y.) | 4240970 | Cells containing fewer than 49 chromosomes probably resulted from excess spreading during hypotonic treatment, since allcells showed apparent trisomy of a small acrocentric. This Save | |
1969 | Whitman, MA; | Discrimination Learning as a Function of MA, IQ, and Institutionalization | Thesis | number of correct responses for the groupswhich were involved in computing these 5 tests are listed in Table 4(see Appendix 2 for average performance and average latency of allcells) Save | ||
1969 | Jacoby, RO; | Studies in canine immunobiology with special reference to mechanisms of lymphoid cell depletion in viral disease | Thesis | In virus-induced tumors, allcells transformed by a given v irus have a common TSTA regardless of the morphology of the tumor or its host of or ig in , but the TSTAs of tumors induced by Save | ||
1969 | Gordon, MW; | Neuronal plasticity and memory | The American journal of orthopsychiatry | 4896079 | Some have been mentioned which are associated with the synaptic mem brane but allcells contain enzymes that direct incoming and outgoing metabolic traffic. Recently, an Save | |
1969 | Grady, CP;Gaudy, AF; | Control Mechanisms Operative in a Natural Microbial Population Selected for Its Ability to Degrade L-Lysine. II. Effects of Fructose and Ribose in Batch Systems | Applied microbiology | 16349866 | Hence, not allcells of the population were capable of utilizing ribose. Since this had not been encountered with the carbon sources previously used, further selection was not carried out. Save | |
1969 | Baron, DN; | Down with plasma. Intracellular chemical pathology studied by analysis of cells of solid tissues, erythrocytes, and leukocytes | Proceedings of the Royal Society of Medicine | 4900112 | For allcells, because of the technical problems involved, it had been usual to consider the cells with their accompanying extracellular fluid as a single tissue. This assumed, not neces Save | |
1968 | Bernheimer, AW; | Cytolytic toxins of bacterial origin. The nature and properties of cytolytic proteins are discussed with emphasis on staphylococcal alpha-toxin | Science (New York, N.Y.) | 4295145 | All cells that are sensitive to streptolysin 0 contain cholesterol as a constituent of their cell membranes, and allcells so far examined that lack membrane cho- Save | |
1968 | Bolognesi, DP;Langlois, AJ;Sverak, L;Bonar, RA;Beard, JW; | In vitro chick embryo cell response to strain MC29 avian leukosis virus | Journal of virology | 4301506 | has disclosed cultural changes of two principal kinds: (i) behavior of the virus-exposed cells paralleling that of the control cultures related presumably to environmental effects on allcells Save | |
1968 | Fielding, S; | Auditory Adaptation: Evidence of Duplexity | Thesis | This 2 0 - ce ll experiment was rep lic a te d fiv e tim es; allcells in one replicat io n were completed b efo re the nex treplicat io n began. Save | ||
1968 | Walters, RA;Petersen, DF; | Radiosensitivity of Mammalian Cells: I. Timing and Dose-Dependence of Radiation-Induced Division Delay | Biophysical Journal | It appears that not only do allcells recover the ability to traverse thelife cycle and divide after irradiation with relatively small doses but do so for significant periods of time without Save | ||
1968 | Bloodworth, JM;Wers, KL; | The ultrastructure of the normal dog adrenal | Journal of anatomy | 5656137 | Adrenal capsule composed of elongated connective tissue cells (CT), basement membrane (BM) which surrounds most but not allcells (arrow), and collagen fibrils (C). x 6500. Fig. Save | |
1967 | Epel, D; | Protein synthesis in sea urchin eggs: a "late" response to fertilization | Proceedings of the National Academy of Sciences of the United States of America | 5232369 | Furthermore, autoradiographic data show that this protein synthesis occurs in allcells of the population, so that the observed incorporation cannot be attributed to the presence of Save | |
1967 | Carstensen, EL;Smearing, RW; | Dielectric properties of osmium-fixed erythrocytes | IEEE transactions on bio-medical engineering | 6078983 | Above 50 iIHz, the effective homiiogeneous conductivity for allcells described in Figs. and 3 is equal to the internal conductivity. The reactance of the imiembrane is negligible above Save | |
1967 | Panteleakis, PN; | STUDIES OF NUCLEOLAR MORPHOLOGY AND CYTOCHEMISTRY AFTER ALTERATION OF RNA, AND PROTEIN SYNTHESIS | Thesis | S ince allcells tr ea te d in thism anner g iv es im ila r results , con tr o ls w ill not be repor te The firsteffectof actin omy cin D on allcells , w ith th eexcep tio nof HEp. No. 2 become d is Save | ||
1967 | Hoff, BJ; | The Cytology and Fertility of Bermudagrass, Cynodon Dactylon (L.) Pers., in Arizona | Thesis | However this was not the case in allcells and in cells from plant 41, with 3 accessory chromo¬somes, sometimes none, one, two or all three accessorychromosomes divided precociously Save | ||
1967 | Tamura, A;Matsumoto, A;Higashi, N; | Purification and chemical composition of reticulate bodies of the meningopneumonitis organisms | Journal of bacteriology | 6025310 | When- almost allcells were infected, they were theni harvested, andthe cells were renioved by centrifugation at 300 X g for 10 nmimi. The organisimis in the superinatanit fluid were Save | |
1967 | Neill, C;Guest, WC; | Effects of Hydroxyurea on Cultured Somatic Cells of the Chinese Hamster, Cricetulus Griseus | Thesis | Allcells within the grid and the cells in some stage of cell division were scored. Ten randomly chosen fields were counted on each replicate. A mitotic index, calculated according to the Save | ||
1966 | Gartland, WJ;Sueoka, N; | Two interconvertible forms of tryptophanyl sRNA in E. coli | Proceedings of the National Academy of Sciences of the United States of America | 5327074 | Induction of cellular DNA synthesis by polyoma virus had so far been observed under conditions where not allcells were productively infected.In contrast with these findings, Sheinin Save | |
1966 | Vogt, M;Dulbecco, R;Smith, B; | Induction of cellular DNA synthesis by polyoma virus. 3. Induction in productively infected cells | Proceedings of the National Academy of Sciences of the United States of America | 4286763 | Induction of cellular DNA synthesis by polyoma virus had so far been observed under conditions where not allcells were productively infected.In contrast with these findings, Sheinin Save | |
1965 | Marcus, PI;Carver, DH; | Hemadsorption-negative plaque test: new assay for rubella virus revealing a unique interference | Science (New York, N.Y.) | 4283964 | In contrast, Echo-ll, polio-I, vaccinia, and influenza B viruses produce gross cytopathic changes in essentially allcells of the monolayer within 24 hours. A noncytopathic, hemadsorbing Save | |
1965 | Barker, CL; | Maternal childrearing history and subsequent sensitivity to shame in college males | Thesis | Level of illu m in atio n scores and level of sound scores co rre la te dpos itiv e ly in allcells , but only one of the eight £ 's was s ig n ific an t. O ther than this , th ere appeared to be no sys Save | ||
1965 | MAC DONALD, C; | AUTORADIOGRAPHY OF THYMIDINE-TRITIUM AND LYSINE-TRITIUM INCORPORATED INTO HUMAN CHROMOSOMES | Thesis | The process of duplication of cellular organellesimplied by the wcell theory" and the notion that allcells are derived from other cells, stands as a funda¬ mental concept of biology. The Save | ||
1964 | ZIEVE, PD;GAMBLE, JL;JACKSON, DP; | EFFECTS OF THROMBIN ON THE POTASSIUM AND ATP CONTENT OF PLATELETS | The Journal of clinical investigation | 14223918 | platelets produced by thrombin apparently was not due to a general effect on allcells, since thrombin did not induce a loss of potassium from red blood cells. The mechanism of action of Save | |
1964 | HINZE, HC;WALKER, DL; | RESPONSE OF CULTURED RABBIT CELLS TO INFECTION WITH THE SHOPE FIBROMA VIRUS. I. PROLIFERATION AND MORPHOLOGICAL ALTERATION OF THE INFECTED CELLS | Journal of bacteriology | 14219036 | Typical cytoplasmic inclu-sions appear at this time in virtually allcells of the infected culture. No other changes in cell morphology are apparent at this time. Eosin and hematoxylin stain. Save | |
1962 | Weiss, JE; | THE EFFECT OF PROFESSIONAL TRAINING, AMOUNT OF INFORMATION, AND CORRECTNESS OF INFORMATION ON ACCURACY OF PREDICTION. | Thesis | d id not d is tr ib u te evenly over allcells , it was n ecessary Save | ||
1960 | Kallenbach, E; | DNA synthesis in the cells of the thymic cortex of the rat as shown in radioautographs after administration of Thymidine-H^ 3. | Thesis | Sainte-Marie and Leblond (1958a) COQ~ted allcells seen in section that is also calottes of cells which were lying with the greater part of their volume outside the section. This procedure Save | ||
1959 | Higgins, TW; | New Polyacrylamide Gel Separators for the Le Clanche Cell | Thesis | P r io r to d is charge the open circuit vo ltage of allcells was measured to avoid te st in g an obviously de fec tiv ecell .The cel ls were prepared ford ischarge by so ld e rin g sh o rt lead Save | ||
1958 | MANDELSTAM, J; | Turnover of protein in growing and non-growing populations of Escherichia coli | The Biochemical journal | 13535591 | From the data available it is not possible to tell whether turnover occurs in allcells of a nongrowing population or whether all proteins are involved. A number of observations, however, Save | |
1957 | Somerville, RL; | In vitro studies of purine nucleotide biosynthesis in rat intestinal mucosa | Thesis | the various forms of living matter pass on to their progeny their various specifically organized patterns of growth and metabolism and are prominent constituents of the nuclei of allcells (2 Save | ||
1955 | ISING, U; | Chromosome studies in Ehrlich mouse ascites cancer after heterologous transplantation through hamsters | British journal of cancer | 13304218 | 4, which also suggests a narrowing of the scatter of the chromo-some numbers, the modal class containing as many as 39 per cent of allcells, as against only 25 per cent in the mouse Save | |
1955 | Angelotti, R; | Studies on the physiological activities of terramycin-resistant and terramycin-susceptible cultures of cheese-ripening strains of Streptococcus lactis | Thesis | Upon exposure to phage, allcells would presumably be sensitive and the number of resist¬ant bacteria obtained from a series of similar samples shouldshow only the fluctuation due to Save | ||
1951 | Deschner, EE; | The Relationship Between Ribonucleic Acid and the Gram Staining Reaction | Thesis | , the morphology of the cells was not changed but some of the cells were completely Gram negative in character, yet no signs of any stages of digestion were present, that is, allcells Save | ||
1951 | Williams, JLD; | Enchondroma of the Nasal Cavities, and its Relationship to Other Cartilaginous Tumours in This Region | Thesis | nantof neop lasm s, us in g th eparasiticfoe tu ses , tera to ma ta and mixed tum ours as s te pp in gs to nes * Thus, al th ough th ereisanaturalfacility forlimitlessg ro w th in near ly allcells Save | ||
1950 | Smith, GM;Regnery, DC; | Inheritance of Sexuality in Chlamydomonas Reinhardi | Proceedings of the National Academy of Sciences of the United States of America | 16578332 | For all species investigated he finds exceptional cases in which allcells produced by a germinating zygote are homothallic (monoecious) instead of all being heterothallic. For zygotes Save | |
1950 | Jehle, H; | Specificity of Interaction Between Identical Molecules | Proceedings of the National Academy of Sciences of the United States of America | 16578331 | For all species investigated he finds exceptional cases in which allcells produced by a germinating zygote are homothallic (monoecious) instead of all being heterothallic. For zygotes Save | |
1941 | Loofbourow, JR;Dwyer, CM;Cronin, AG; | Proliferation-promoting intercellular hormones: Evidence for their production by living cells as a response to injury | The Biochemical journal | 16747427 | Both were then irradiated in quartz test tubes with full ultraviolet until allcells in the unboiled suspension were killed (methylene.blue test). The irradiation time in the experiment Save | |
1934 | Tryon, R; | A correlation form with absolute checks. | Journal of Experimental Psychology | In each row of quadrant 1, Multiply the frequency in each cell tines the nuwber in the upper corner of the cell, ant) SUB all these for allcells in that row, placing the total In the proper Save | ||
1929 | McDonald, E; | CELLULAR DIVISION IN RELATION TO CANCER | Science (New York, N.Y.) | 17733743 | The cell reacts as a unit and there are certain phenomena which are common to allcells. There are regularities in the complexity of cell phenomena which make it possible to study cell Save | |
1926 | Orahovats, D; | The spleen and the resistance of red cells | The Journal of physiology | 16993804 | First a group which is most resistant and which forms about 10 pc of allcells, then a group to which the greatest part of the red cells belong, about 80 pc, with a middle resistance, and a Save | |
1920 | Mott, FW; | Studies in the Pathology of Dementia Praecox | Proceedings of the Royal Society of Medicine | 19981338 | This applies to allcells including those of the olivary body, although the pigment in this kind of cell is finer. The large cells show the lipoid granules better. Unstained Specimen of Medulla Save | |
1911 | Paine, A;Nicholson, GW; | SURGICAL INTERFERENCE IN CANCER: (AN EXPERIMENTAL STUDY.) | British medical journal | 20765743 | As allcells have an age limit, at the end of which they die and towards the close of whichthey become old and their activities diminish, itfollows that the isolated nodules, which consist Save | |
1905 | MARCY, H; | THE CURE OF HERNIA. | Journal of the American Medical Association | for 30 minutes and not allcells being killed during one hour at this temperature. This may explain the fact that the virus is not always killed by cooking the meat. The organism probably Save | ||
1874 | Mahomed, FA; | The Relation between Arterial Tension and Albuminuria | British medical journal | 20747530 | These are cast off in great numbers, and, blocking the tubules, form the large white kidney; here they undergo the change common to allcells after their death-namely, molecular Save | |
0 | Authors | Title | Journal | 0 | Snippet |
Join Discovery Life Sciences at I/O Summit Europe 2024 April 23-25, 2024 | Hilton London Canary Wharf, London, England
Join Discovery Life Sciences in Booth #11 at CB & CDx London 2024 April 24-25, 2024 | Millennium Gloucester Hotel London Kensington, London, England
Join Discovery Life Sciences at Cyto Conference 2024 May 4-8, 2024 | Edinburgh International Convention Center, Edinburgh, Scotland
Copyright © 2024 Discovery Life Sciences. All rights reserved.
Designed & Developed by Altitude Marketing