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Publications

Discovery has worked with hundreds of researchers on thousands of projects to help uncover valuable insights that accelerate research and development programs across several disease indications, including cancer, infectious disease, autoimmune disease, women’s health, cardiovascular disease, inflammatory diseases and many others.

YearAuthorsTitleJournalPubMed IDSnippetLink
2023Bellet-Ezquerra, M;Pardo, P;Fasani, R;Villacampa, G;Portu, M;Serna, G;Joval, L;Petit, A;Ortega, A;Jimenez, J;Zamora, E;Gil, M;Gomez, L;Viaplana, C;Gallego, F;Cámara, V;Manich, C;Simon, S;Nuciforo, P;62P KI67 in ER+HER2-negative pure invasive lobular breast carcinoma (ILC). What’s the best Ki67 threshold to differentiate prognosis?ESMO Opena, Novartis, Daiichi Sankyo; Non-Financial Interests, Invited Speaker: SOLTI. P.G. Nuciforo: Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal,Full Paper
2023Cieniewicz, B;Bhatta, A;Torabi, D;Baichoo, P;Saxton, M;Arballo, A;Nguyen, L;Thomas, S;Kethar, H;Kukutla, P;Shoaga, O;Yu, B;Yang, Z;Fate, M;Oliveira, E;Ning, H;Corey, L;Corey, D;Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti-tumor responses and phagocytic uptake of tumor-associated antigen for T cell cross-presentationMolecular therapy : the journal of the American Society of Gene Therapy37194236Healthy donor T cells were positively selected from fresh leukopak provided by AllCells (Alameda, CA) by using anti-CD4 and CD8 antibodies through the Prodigy TCT program. All related reagents and instruments were purchased from Miltenyi. After selecFull Paper
2023Overbeck, TR;Reiffert, A;Schmitz, K;Rittmeyer, A;Körber, W;Hugo, S;Schnalke, J;Lukat, L;Hugo, T;Hinterthaner, M;Reuter-Jessen, K;Schildhaus, HU;NTRK Gene Fusions in Non-Small-Cell Lung Cancer: Real-World Screening Data of 1068 Unselected PatientsCancers37296928r or speaker from: Amgen, AstraZeneca, Bayer, Daiichi-Sankyo, Diaceutics, Incyte, Janssen-Cilag, Eli Lilly, Merck Sharp and Dohme, Novartis Pharma, Pfizer, PharmaMar, Roche Pharma, Servier, Stemline, Targos GmbH, and ZytoVision. A.R. received grantsFull Paper
2023Wagner, R;McKindles, K;Bullerjahn, G;Effects of water movement and temperature on Rhizophydium infection of Planktothrix in a shallow hypereutrophic lakeFrontiers in Microbiologysent to HudsonAlpha Discovery Life Sciences (Hudson, AL, United States) for sequencing. Amplicon sequencing and QC was performed by HudsonAlpha Discovery Life Save;- Discovery Life Sciences (Hudson, AL, United States) for sequencing. Amplicon seFull Paper
2023de Vries, EGE;Rüschoff, J;Lolkema, M;Tabernero, J;Gianni, L;Voest, E;de Groot, DJA;Castellano, D;Erb, G;Naab, J;Donica, M;Deurloo, R;van der Heijden, MS;Viale, G;Phase II study (KAMELEON) of single-agent T-DM1 in patients with HER2-positive advanced urothelial bladder cancer or pancreatic cancer/cholangiocarcinomaCancer medicine37119523Department of Medical Oncology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.++Targos Molecular Pathology GmbH, Kassel, Germany.++Department of Medical Oncology, Erasmus MC Cancer Institute, Rotterdam, TheFull Paper
2023Wang, S;Huang, Z;Liu, Y;Sun, H;Zhou, Y;Shi, J;Sustainably released nanoparticle-based rhynchophylline limits pulmonary fibrosis by inhibiting the TEK-PI3K/AKT signaling pathwayTranslational lung cancer research37057119/tlcr-22-675/rc). Methods Solution groups For better apply to the clinic, 0.9% saline solution was used as the solvent. Because of the extremely low concentration for cell, 1 mg of Rhy (CAS 76-66-4; Allcells, Shanghai, China) was directly dissolvedFull Paper
2023Naik, NN;Vadloori, B;Poosala, S;Srivastava, P;Coecke, S;Smith, A;Akhtar, A;Roper, C;Radhakrishnan, S;Bhyravbhatla, B;Damle, M;Pulla, VK;Hackethal, J;Horland, R;Li, AP;Pati, F;Singh, MS;Occhetta, P;Bisht, R;Dandekar, P;Bhagavatula, K;Pajkrt, D;Johnson, M;Weber, T;Huang, J;Hysenaj, L;Mallar, B;Ramray, B;Dixit, S;Joshi, S;Kulkarni, M;Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Third International Conference on 3Rs Research and Progress, Vishakhapatnam, 2022Alternatives to laboratory animals : ATLA37282515Dr Li highlighted the discovery focus of Discovery Life Sciences LLC, namely the liver and intestine. He further described the properties of their two major novel human in SaveFull Paper
2023Guler, S;DiPoto, MC;Crespo, A;Caldwell, R;Doerfel, B;Grossmann, N;Ho, K;Huck, B;Jones, CC;Lan, R;Musil, D;Potnick, J;Schilke, H;Sherer, B;Simon, S;Sirrenberg, C;Zhang, Z;Liu-Bujalski, L;Selective Wee1 Inhibitors Led to Antitumor Activity In Vitro and Correlated with MyelosuppressionACS medicinal chemistry letters37197456We acknowledge Bettina Hoelke, Brian Dill, Ioannis Gounaris, and Emer Clarke (ReachBio) for their technical support and discussionsFull Paper
2023Tang, PZ;Ding, B;Reyes, C;Papp, D;Potter, J;Target-seq: single workflow for detection of genome integration site, DNA translocation and off-target eventsBioTechniques37161298Peripheral blood mononuclear cells (PBMCs) were purified from leukopak (ALLCELLS, CA, USA) using CTS™ Rotea™ Counterflow Centrifugation System (Thermo Fisher Scientific, MA, USA) and were then activated for 3 days to enrich the T cells (at 1 × 106 ceFull Paper
2023Singh, P;MSC and HSPC Coculture: Mimicking Ex Vivo Bone Marrow NicheMethods in molecular biology (Clifton, N.J.)36255702CD34+ cells with micromagnetic beads-conjugated CD34 antibody and placing with the labeled cells into a magnetic field according to the specific manual instruction. Always use fresh BM samples (within 30 h of withdrawal) for CD34+ cell isolation. HumFull Paper
2023Simmons, KT;Chan, J;Hussain, S;Rose, EL;Markham, K;Byun, TS;Panicker, S;Parry, GC;Storek, M;Anti-C1s humanized monoclonal antibody SAR445088: A classical pathway complement inhibitor specific for the active form of C1sClinical immunology (Orlando, Fla.)37149117First, 1 mL of type O- hRBCs (Allcells, Alameda, CA) was washed in 4.5 mL GVB++ by centrifuging at 600 g for 5 min. The wash step was repeated three times and the cells were resuspended to a final volume of 5 mL in GVB++ after the third washFull Paper
2023Sikkema, L;Ramírez-Suástegui, C;Strobl, DC;Gillett, TE;Zappia, L;Madissoon, E;Markov, NS;Zaragosi, LE;Ji, Y;Ansari, M;Arguel, MJ;Apperloo, L;Banchero, M;Bécavin, C;Berg, M;Chichelnitskiy, E;Chung, MI;Collin, A;Gay, ACA;Gote-Schniering, J;Hooshiar Kashani, B;Inecik, K;Jain, M;Kapellos, TS;Kole, TM;Leroy, S;Mayr, CH;Oliver, AJ;von Papen, M;Peter, L;Taylor, CJ;Walzthoeni, T;Xu, C;Bui, LT;De Donno, C;Dony, L;Faiz, A;Guo, M;Gutierrez, AJ;Heumos, L;Huang, N;Ibarra, IL;Jackson, ND;Kadur Lakshminarasimha Murthy, P;Lotfollahi, M;Tabib, T;Talavera-López, C;Travaglini, KJ;Wilbrey-Clark, A;Worlock, KB;Yoshida, M;Lung Biological Network Consortium, ;van den Berge, M;Bossé, Y;Desai, TJ;Eickelberg, O;Kaminski, N;Krasnow, MA;Lafyatis, R;Nikolic, MZ;Powell, JE;Rajagopal, J;Rojas, M;Rozenblatt-Rosen, O;Seibold, MA;Sheppard, D;Shepherd, DP;Sin, DD;Timens, W;Tsankov, AM;Whitsett, J;Xu, Y;Banovich, NE;Barbry, P;Duong, TE;Falk, CS;Meyer, KB;Kropski, JA;Pe'er, D;Schiller, HB;Tata, PR;Schultze, JL;Teichmann, SA;Misharin, AV;Nawijn, MC;Luecken, MD;Theis, FJ;An integrated cell atlas of the lung in health and diseaseNature medicine3729121419-ms2/?ds=full&meta=SampleName, https://figshare.com/articles/dataset/Single-cell_RNA-Seq_of_human_primary_lung_and_bronchial_epithelium_cells/11981034/1, https://covid19.lambrechtslab.org/downloads/Allcells.counts.rds, https://s3.amazonaws.com/dp-lFull Paper
2023Sternberg, CN;Petrylak, DP;Bellmunt, J;Nishiyama, H;Necchi, A;Gurney, H;Lee, JL;van der Heijden, MS;Rosenbaum, E;Penel, N;Pang, ST;Li, JR;García Del Muro, X;Joly, F;Pápai, Z;Bao, W;Ellinghaus, P;Lu, C;Sierecki, M;Coppieters, S;Nakajima, K;Ishida, TC;Quinn, DI;FORT-1: Phase II/III Study of Rogaratinib Versus Chemotherapy in Patients With Locally Advanced or Metastatic Urothelial Carcinoma Selected Based on FGFR1/3 mRNA ExpressionJournal of clinical oncology : official journal of the American Society of Clinical Oncology36240478ted prevalence of PIK3CA and/or RAS resistance mutations, we reconfirmed absence or presence in all enrolled patients using a targeted Illumina MiSeq panel (Illumina, Inc, San Diego, CA) performed by TARGOS Molecular Pathology GmbH (Kassel, Germany).Full Paper
2023Ho, C;Fromm, J;Fang, M;Till, B;Shadman, M;Cowan, A;Lynch, R;Wu, V;Voutsinas, J;Rasmussen, H;Blue, K;Ujjani, C;Shustov, A;Cassaday, R;Gopal, A;Smith, S;Long-term efficacy and safety of pembrolizumab with R-CHOP as first-line therapy for DLBCL.Journal of Clinical OncologyAdditionally, prognostic factors including tumor PD-L1 expression (Qualtek/Merck) were re-analyzed. Results: 30 patients were treated (13/30 with IPI 3-5). Median follow SaveFull Paper
2023Zhang, Y;Zhao, Z;Huang, LA;Liu, Y;Yao, J;Sun, C;Li, Y;Zhang, Z;Ye, Y;Yuan, F;Nguyen, TK;Garlapati, NR;Wu, A;Egranov, SD;Caudle, AS;Sahin, AA;Lim, B;Beretta, L;Calin, GA;Yu, D;Hung, MC;Curran, MA;Rezvani, K;Gan, B;Tan, Z;Han, L;Lin, C;Yang, L;Molecular mechanisms of snoRNA-IL-15 crosstalk in adipocyte lipolysis and NK cell rejuvenationCell metabolism37329887Healthy and obese human serum Coriell Biorepository See Table S1 Human breast cancer tumor tissues and paired plasma samples Discovery Life Sciences See Table S2 Primary human pre-adipocytes Zen-Bio See Table S3Full Paper
2023Libreros, S;Nshimiyimana, R;Lee, B;Serhan, CN;Infectious Neutrophil deployment is regulated by Resolvin D4Blood37295018Fresh BM aspirates from deidentified individuals were purchased from AllCells, Inc (Alameda, CA) with Mass General Brigham investigational review board protocol no. 1999P0001279. For information on donor demographics, see Supplemental Table-1. HumanFull Paper
2023Dunbar, AJ;Kim, D;Lu, M;Farina, M;Bowman, RL;Yang, JL;Park, Y;Karzai, A;Xiao, W;Zaroogian, Z;O'Connor, K;Mowla, S;Gobbo, F;Verachi, P;Martelli, F;Sarli, G;Xia, L;Elmansy, N;Kleppe, M;Chen, Z;Xiao, Y;McGovern, E;Snyder, J;Krishnan, A;Hill, C;Cordner, K;Zouak, A;Salama, ME;Yohai, J;Tucker, E;Chen, J;Zhou, J;McConnell, T;Migliaccio, AR;Koche, R;Rampal, R;Fan, R;Levine, RL;Hoffman, R;CXCL8/CXCR2 signaling mediates bone marrow fibrosis and is a therapeutic target in myelofibrosisBlood36800567ple was independently verified by a hematopathologist (W.X.) or pulled directly from clinical pathology reports at the time of sample collection. Deidentified, healthy CD34+ cells were purchased from AllCells. CD34+ selection was carried out using FiFull Paper
2023Kiridena, S;Wijayaratna, U;Levon, E;Moschella, P;Pirrallo, R;Tzeng, T;Anker, J;X?Ray Visualized Sensors for Peritoneal Dialysis Catheter InfectionAdvanced Functional MaterialsDeidentified human peritoneal fluid was obtained from Discovery Life Sciences, Huntsville, AL, a commercial biospecimen source. Tantalum beads (0.394 mm diameter) SaveFull Paper
2023Tarafder, S;Ghataure, J;Langford, D;Brooke, R;Kim, R;Eyen, SL;Bensadoun, J;Felix, JT;Cook, JL;Lee, CH;Advanced bioactive glue tethering Lubricin/PRG4 to promote integrated healing of avascular meniscus tearsBioactive materials37214259?S-encapsulating TGF?3 (R&D systems) (2.5 ?g per 500 mg PLGA) was applied to the incised tissues. Then the meniscus explants were placed on top of monolayer-cultured P2 - P3 syMSCs or hBMSCs (AllCells, Alameda, CA) at 80-90% confluence per our previoFull Paper
2023Sarno, J;Domizi, P;Liu, Y;Merchant, M;Pedersen, CB;Jedoui, D;Jager, A;Nolan, GP;Gaipa, G;Bendall, SC;Bava, FA;Davis, KL;Dasatinib overcomes glucocorticoid resistance in B-cell acute lymphoblastic leukemiaNature communications37217509Healthy human bone marrow samples (n = 3 for RNA-Seq, n = 4 for CyTOF) were purchased through AllCells. De-identified bone marrow samples from pediatric patients SaveFull Paper
2023Yang, Y;Yang, H;Alcaina, Y;Puc, J;Birt, A;Vedvyas, Y;Gallagher, M;Alla, S;Riascos, MC;McCloskey, JE;Du, K;Gonzalez-Valdivieso, J;Min, IM;de Stanchina, E;Britz, M;von Hofe, E;Jin, MM;Inducible expression of interleukin-12 augments the efficacy of affinity-tuned chimeric antigen receptors in murine solid tumor modelsNature communications37045815quoted and stored at ?80?°C. CAR-T cells were generated by transducing activated T cells with lentivirus. Primary T cells were enriched from commercially obtained leukopaks from healthy donors (AllCells) via MACS separation using CD4 (Miltenyi BioteFull Paper
2023Lee, JY;Jonus, HC;Sadanand, A;Branella, GM;Maximov, V;Suttapitugsakul, S;Schniederjan, MJ;Shim, J;Ho, A;Parwani, KK;Fedanov, A;Pilgrim, AA;Silva, JA;Schnepp, RW;Doering, CB;Wu, R;Spencer, HT;Goldsmith, KC;Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastomaCell reports. Medicine37343516Bacterial and virus strains ------------------------- Stbl3 Competent Cells Spencer Lab N/A ------------------------- Biological samples ------------------------- Healthy PBMC AllCells LP,CR,MNC, 10M COG-424s (Neuroblastoma PDX) ChiFull Paper
2023Chen, SS;Barrientos, JC;Ferrer, G;King-Richards, M;Chen, YJ;Ravichandran, P;Ibrahim, M;Kieso, Y;Waters, S;Kutok, JL;Peluso, M;Sharma, S;Weaver, DT;Pachter, JA;Rai, KR;Chiorazzi, N;Duvelisib Eliminates CLL B Cells, Impairs CLL-Supporting Cells, and Overcomes Ibrutinib Resistance in a Xenograft ModelClinical cancer research : an official journal of the American Association for Cancer Research37071496Human PBMCs obtained from whole blood of CLL donors were purchased from a commercial source (Bioreclamation IVT or ALLCELLS) were resuspended (1 × 10 6 cells/ SaveFull Paper
2023Xiang, J;Liu, Q;Shaknovich, R;Venn, O;Abstract 779: Clonal B-cell expansion and the potential challenges to blood-based early cancer detectionCancer Researchht tubes of frozen buffy coat for a young healthy donor (18-35; no pre-existing health conditions; nonsmoker; viral negative) was sourced commercially (Discovery Life Sciences, Huntsville, AL, USA) and used as the backgrouFull Paper
2023Fahl, S;Colwell, K;Smith, C;Ott, C;Moore, A;Williams, K;Abstract 602: Single cell evaluation of intratumoral B cells across solid tumor indicationsCancer Research1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, HuntsvillFull Paper
2023Li, A;Wei, H;Abstract 5338: A novel in vitro assay to evaluate the roles of hepatic metabolism on anticancer drug safety and efficacyCancer Research1Discovery Life Sciences LLC, Columbia, MD.++1Discovery Life Sciences LLC, Columbia, MDFull Paper
2023Roy, G;Kadekar, P;Douglas, L;Frappier, M;Currie, J;Dhillon, J;Cesarone, G;Siderits, R;Kirchner, K;Demeule, M;Marsolais, C;Abstract 3942: Differential expression of a novel transport receptor, SORT1 (sortilin), in cancer versus healthy tissues that can be utilized for targeted delivery of anti-cancer drugsCancer Research1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec,Full Paper
2023Cheng, C;Chen, H;Kirchner, K;Cesarone, G;Abstract 2763: Characterization of a novel immunohistochemistry (IHC) assay for CEACAM5 using a commercial antibodyCancer Research[Abcam], and 327 [Sino Biological]) to evaluate CEACAM5 detection in formalin-fixed, paraffin-embedded (FFPE) human cancer tissues from Discovery Life Sciences SaveFull Paper
2023Zielinski, D;Vogtmann, R;Siemon, A;Koppel, C;Schipper, C;Tereshchenko, A;Platero, S;Schildhaus, H;Abstract 1018: Multiplex immunofluorescence and image analysis to investigate the role of the immune contexture and fibroblast activation for tumor cell budding in colorectal cancerCancer Research1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, HuntsvillFull Paper
2023Kumar, A;Taghi Khani, A;Duault, C;Aramburo, S;Sanchez Ortiz, A;Lee, SJ;Chan, A;McDonald, T;Huang, M;Lacayo, NJ;Sakamoto, KM;Yu, J;Hurtz, C;Carroll, M;Tasian, SK;Ghoda, L;Marcucci, G;Gu, Z;Rosen, ST;Armenian, S;Izraeli, S;Chen, CW;Caligiuri, MA;Forman, SJ;Maecker, HT;Swaminathan, S;Intrinsic suppression of type I interferon production underlies the therapeutic efficacy of IL-15-producing natural killer cells in B-cell acute lymphoblastic leukemiaJournal for immunotherapy of cancer37217248opoietic Tissue Biorepository and University of Pennsylvania Stem Cell and Xenograft Core after informed consent per Institutional Review Board policies. Age-matched healthy BMMCs were purchased from AllCells (Alameda, California, USA) and Stem CellFull Paper
2023Nirschl, CJ;Brodkin, HR;Domonkos, C;Dwyer, CJ;Hicklin, DJ;Ismail, N;Seidel-Dugan, C;Steiner, P;Steuert, Z;Sullivan, JM;Winston, WM;Salmeron, A;mWTX-330, an IL 12 INDUKINE Molecule, Activates and Reshapes Tumor-infiltrating CD8+ T and NK Cells to Generate Antitumor ImmunityCancer immunology research37074216Dissociated human tumor samples were purchased from Discovery Life Sciences and included the following indications: bladder cancer, glioblastoma multiforme, gastric SaveFull Paper
2023Diwanji, R;O'Brien, NA;Choi, JE;Nguyen, B;Laszewski, T;Grauel, AL;Yan, Z;Xu, X;Wu, J;Ruddy, DA;Piquet, M;Pelletier, MR;Savchenko, A;Charette, L;Rodrik-Outmezguine, V;Baum, J;Millholland, JM;Wong, CC;Martin, AM;Dranoff, G;Pruteanu-Malinici, I;Cremasco, V;Sabatos-Peyton, C;Jayaraman, P;Targeting the IL1? Pathway for Cancer Immunotherapy Remodels the Tumor Microenvironment and Enhances Antitumor Immune ResponsesCancer immunology research37040466Dissociated human microsatellite-stable colorectal carcinoma (MSS-CRC) samples were obtained from Discovery Life Sciences from five donors. Between 25,000 and SaveFull Paper
2023Huang, W;Lin, W;Chen, B;Zhang, J;Gao, P;Fan, Y;Lin, Y;Wei, P;NFAT and NF-?B dynamically co-regulate TCR and CAR signaling responses in human T cellsCell reports37347664K562 Laboratory of Wendell Lim N/A Jurkat Laboratory of Arthur Weiss N/A Human PBMC ALLCELLS PB004F-CFull Paper
2023Raj, N;Chan, JA;Wang, SJ;Aggarwal, RR;Calabrese, S;DeMore, A;Fong, L;Grabowsky, J;Hope, TA;Kolli, KP;Mulvey, CK;Munster, PN;Perez, K;Punn, S;Reidy-Lagunes, D;Von Fedak, S;Zhang, L;Bergsland, EK;Pembrolizumab alone and pembrolizumab plus chemotherapy in previously treated, extrapulmonary poorly differentiated neuroendocrine carcinomasBritish journal of cancer37208512Background To date, single-agent immune checkpoint inhibitor (CPI) therapy has proven to be ineffective against biomarker-unselected extrapulmonary poorly SaveFull Paper
2023Reissig, TM;Tzianopoulos, I;Liffers, ST;Rosery, VK;Guyot, M;Ting, S;Wiesweg, M;Kasper, S;Meister, P;Herold, T;Schmidt, HH;Schumacher, B;Albers, D;Markus, P;Treckmann, J;Schuler, M;Schildhaus, HU;Siveke, JT;Smaller panel, similar results: genomic profiling and molecularly informed therapy in pancreatic cancerESMO open37148593stock ownership: none; honoraries for CME presentations: Amgen, Boehringer Ingelheim, Bristol-Myers Squibb, Janssen, Novartis; research funding to institution: AstraZeneca, Bristol Myers-Squibb. HUS: Targos Molecular Pathology, Inc. (employment); RocFull Paper
2023Han, R;Guo, H;Shi, J;Wang, H;Zhao, S;Jia, Y;Liu, X;Li, J;Cheng, L;Zhao, C;Li, X;Zhou, C;Tumour microenvironment changes after osimertinib treatment resistance in non-small cell lung cancerEuropean journal of cancer (Oxford, England : 1990)37320935Fresh PBMCs of three healthy donors were purchased from Allcells (China). A total of 2 × 10 6 PBMC cells/well were seeded into 24-well plated and were treated with: SaveFull Paper
2023Geoerger, B;Marshall, LV;Nysom, K;Makin, G;Bouffet, E;Defachelles, AS;Amoroso, L;Aerts, I;Leblond, P;Barahona, P;Van-Vlerken, K;Fu, E;Solca, F;Lorence, RM;Ziegler, DS;Afatinib in paediatric patients with recurrent/refractory ErbB-dysregulated tumours: Results of a phase I/expansion trialEuropean journal of cancer (Oxford, England : 1990)37178647Biomarker assays were performed centrally by TARGOS Molecular Pathology GmbH using tissue samples from archived tumour blocks, or tissue sections [20]. SaveFull Paper
2023Robert, ME;Rüschoff, J;Jasani, B;Graham, RP;Badve, SS;Rodriguez-Justo, M;Kodach, LL;Srivastava, A;Wang, HL;Tang, LH;Troncone, G;Rojo, F;Van Treeck, BJ;Pratt, J;Shnitsar, I;Kumar, G;Karasarides, M;Anders, RA;Erratum to High Interobserver Variability Among Pathologists Using Combined Positive Score to Evaluate PD-L1 Expression in Gastric, Gastroesophageal Junction, and Esophageal Adenocarcinoma [Modern Pathology 36(5) (2023) 100154]Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc37327723Yale University School of Medicine, New Haven, Connecticut. Electronic address: marie.robert@yale.edu.++Discovery Life Sciences, Hesse, Germany.++Discovery Life Sciences, Hesse, Germany.++Mayo Clinic, Rochester, Minnesota.++Emory University School ofFull Paper
2023Dong, Q;Tong, M;Yu, X;Wang, L;Ao, J;Guan, D;Tang, Y;Liu, J;Long, L;Tong, Y;Fang, S;Zhou, H;Huang, Y;Gong, L;Lou, L;Huang, W;Carbohydrate Strengthens the Immunotherapeutic Effect of Small-Molecule PD-L1 InhibitorsJournal of medicinal chemistry37226718Stimulator cells from donor A and responder cells from donor B constitute the mixed lymphocyte reaction (MLR). Pheral Blood Mononuclear Cells (PBMCs) were purchased from Allcells (Shanghai) and used following the instruction of the manufacturer. DC cFull Paper
2023Papakonstantinou, A;Napoli, S;Joval, L;Butjosa-Espin, M;Malavila, A;Puy, M;Pimentel, I;Zamora, E;Torres, P;Rodas, N;Manich, C;Seoane, J;Nonell, L;Nuciforo, P;Oliveira, M;19P Tumor-associated microbiome composition and response to neoadjuvant chemotherapy (NACT) in early triple-negative breast cancer (TNBC)ESMO Openncer Research (AACR). P.G. Nuciforo: Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. M. OFull Paper
2023Akiyama, H;Carter, BZ;Andreeff, M;Ishizawa, J;Molecular Mechanisms of Ferroptosis and Updates of Ferroptosis Studies in Cancers and LeukemiaCells37190037Another study demonstrates that progestin and adipoQ receptor 3 (PAQR3) enhances erastin- as well as RSL3-induced ferroptosis in ALLcells through the degradation of SaveFull Paper
2023Chaand, M;Fiore, C;Johnston, B;D'Ippolito, A;Moon, DH;Carulli, JP;Shearstone, JR;Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressorCommunications biology37316562Fetal CB-derived CD34+ cells (AllCells, LLC) were transduced with concentrated lentivirus at an MOI = 1. Transduced cells were selected in the presence of 2 µg/mL SaveFull Paper
2023Lin, W;Singh, V;Springer, R;Choonoo, G;Gupta, N;Patel, A;Frleta, D;Zhong, J;Owczarek, T;Decker, C;Macdonald, L;Murphy, A;Thurston, G;Mohrs, M;Ioffe, E;Lu, YF;Human CD4 cytotoxic T lymphocytes mediate potent tumor control in humanized immune system miceCommunications biology37185301from fetal liver or cord blood, respectively. Fetal liver CD34+ HSPC were obtained from Advanced Biosciences Resources (Alameda, CA) with proper consent66 and cord blood CD34+ HSPC were obtained from AllCells, HemaCare, or STEMCELL Technologies. HumaFull Paper
2023Maden, M;Polvadore, T;Polanco, A;Barbazuk, WB;Stanley, E;Osteoderms in a mammal the spiny mouse Acomys and the independent evolution of dermal armoriScience37378333na NovaSeq6000 platform to yield nearly 898 million paired-end, 150 bp reads (mean of 74.8 M read pairs (s = 9.1 M read pairs) per library). Library construction and sequencing were performed by HudsonAlpha Discovery in Huntsville, Alabama, USA. QuaFull Paper
2023Egelston, CA;Guo, W;Yost, SE;Ge, X;Lee, JS;Frankel, PH;Cui, Y;Ruel, C;Schmolze, D;Murga, M;Tang, A;Martinez, N;Karimi, M;Somlo, G;Lee, PP;Waisman, JR;Yuan, Y;Immunogenicity and efficacy of pembrolizumab and doxorubicin in a phase I trial for patients with metastatic triple-negative breast cancerCancer immunology, immunotherapy : CII37294342Biomarker Working Group on Breast Cancer Guidelines [18]. PD-L1 was determined by QualTek Molecular Laboratory (Goleta, CA) using immunohistochemistry (IHC) with 22C3 antibody (Merck & Co, Kenilworth, NJ). PD-L1 was positive if membrane staining wasFull Paper
2023Becker, SA;Petrich, BG;Yu, B;Knight, KA;Brown, HC;Raikar, SS;Doering, CB;Spencer, HT;Enhancing the effectiveness of ?? T cells by mRNA transfection of chimeric antigen receptors or bispecific T cell engagersMolecular therapy oncolytics37387794ed from healthy donor blood through the Children’s Clinical Translational Discovery Core at Emory University under the core’s approved institutional review board protocol or ordered directly from AllCells. PBMCs were isolated from fresh blood using FFull Paper
2023Chervin, AS;Stone, JD;Konieczna, I;Calabrese, KM;Wang, N;Haribhai, D;Dong, F;White, MK;Rodriguez, LE;Bukofzer, GT;Ellis, PA;Cosgrove, C;Hecquet, C;Clarin, JD;Palma, JP;Reilly, EB;ABBV-184: A novel survivin-specific TCR/CD3 bispecific T cell engager is active against both solid tumor and hematological malignanciesMolecular cancer therapeutics37294945Primary frozen viable PBMCs from AML patients and including AML blasts were purchased from Discovery Life Sciences and verified for HLA-A2 expression by staining with the HLA-A2 specific monoclonal antibody BB7.2 and detecting by flow cytometryFull Paper
2023Korntner, SH;Di Nubila, A;Gaspar, D;Zeugolis, DI;Macromolecular crowding in animal component-free, xeno-free and foetal bovine serum media for human bone marrow mesenchymal stromal cell expansion and differentiationFrontiers in bioengineering and biotechnology36949882ed. 2.2 Isolation and expansion of hBMSCs Fresh human whole bone marrow from the iliac crest of two different donors (donor 1: female, 22 years old; donor 2: male, 25 years old) was purchased from AllCells (United States) and hBMSCs were isolated uFull Paper
2023Dempsey, MP;Andersen, KE;Wells, BM;Taylor, MA;Cashman, CL;Conrad, LB;Kearney, CA;Conklin, MB;Via, ER;Doe, EM;Komirisetty, R;Dearborn, S;Reddy, ST;Farias-Eisner, R;Stability Characterization of the Novel Anti-Cancer HM-10/10 HDL-Mimetic PeptideInternational journal of molecular sciences3737320382043-96OD), one plate per lot, were obtained from In Vitro ADMET Laboratories (Malden, MA, USA). The transport medium was replaced with 200 ?L of warm (37 C) SaveFull Paper
2023El Ouardi, M;Tamarit, L;Vayá, I;Miranda, MA;Andreu, I;Cellular photo(geno)toxicity of gefitinib after biotransformationFrontiers in pharmacology37351506UPV-CSIC, Universitat Politècnica de València, Valencia, Spain 2 Unidad Mixta de Investigación UPV- IIS La Fe, Hospital Universitari i Politècnic La Fe, Valencia, Spain Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States Reviewed byFull Paper
2023Takei, S;Kinoshita, H;Jamal, M;Kumihashi, M;Yamashita, T;Tanaka, E;Kawahara, S;Abe, H;Tsutsui, K;Kimura, S;Case report: Fatal poisoning caused by additive effects of two barbituratesFrontiers in pharmacology37292155Medicine, Faculty of Medicine, Kagawa University, Takamatsu, Japan 2 Biodesign Inc, Tokyo, Japan 3 Kagawa Prefectural University of Health Sciences, Takamatsu, Kagawa, Japan Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States ReviewFull Paper
2023Li, S;Zhan, J;Wang, Y;Oduro, PK;Owusu, FB;Zhang, J;Leng, L;Li, R;Wei, S;He, J;Wang, Q;Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery toneFrontiers in pharmacology37234713vestigated. The EC50 and Rmax (maximal vasorelaxation) values were then calculated. 2.8 Cell culture and western blotting analysis Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells Biotech Co., Ltd. (Shanghai, China). TheFull Paper
2023Greenstein, AE;Hunt, HJ;The glucocorticoid receptor modulator relacorilant reverses the immunosuppressive effects of cortisolInternational immunopharmacology37230031GR was assessed using a rabbit monoclonal antibody clone D8H2 from Cell Signaling Technology (Cat#3660S) as described previously [41]. CD3 (Dako, A0452), FOXP3 (eBioscience 14-4777), CD57 (ThermoFisher, MA5-12008), and PD-1 (Abcam, ab53587) were acquFull Paper
2023Cyr, MG;Wilson, HD;Spierling, AL;Chang, J;Peng, H;Steinberger, P;Rader, C;Concerted Antibody and Antigen Discovery by Differential Whole-cell Phage Display Selections and Multi-omic Target DeconvolutionJournal of molecular biology37019174Fresh healthy donor PBMCs were bought from AllCells and cryopreserved prior to use with 50% FBS, 10% DMSO in RPMI-1640 medium. SaveFull Paper
2023Isaioglou, I;Aldehaiman, MM;Li, Y;Lahcen, AA;Rauf, S;Al-Amoodi, AS;Habiba, U;Alghamdi, A;Nozue, S;Habuchi, S;Salama, KN;Merzaban, JS;CD34+ HSPCs-derived exosomes contain dynamic cargo and promote their migration through functional binding with the homing receptor E-selectinFrontiers in cell and developmental biology37181754O2 concentration of 5% v/v. The cell cultures were passaged frequently to maintain the cells at 0.8 × 106 cells/ml. Primary human AML CD34+ cells from Mobilized Peripheral Blood were purchased from AllCells, while Primary Human CD34+ cells from MobiFull Paper
2023Basu, A;Champagne, RN;Patel, NG;Nicholson, ED;Weiss, RJ;TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growthThe Journal of biological chemistry37061003in Table S4. RNA sequencing and differential gene expression analysis Total RNA extracted from wildtype and knockout cell lines was submitted for library preparation and next-generation sequencing (HudsonAlpha Discovery). Raw RNA sequencing data wasFull Paper
2023Spriano, F;Aresu, L;Cascione, L;Risi, G;Arribas, AJ;Napoli, S;Forster-Gross, N;Bachmann, F;Engelhardt, M;Lane, H;Bertoni, F;The microtubule-targeted agent lisavanbulin (BAL101553) shows anti-tumor activity in lymphoma modelsAmerican journal of cancer research37293172(TMA) (LY1001D and LY2086B, TissueArray.com) were stained for EB1 using a CE-marked immunohistochemistry Clinical Trial Assay (Discovery Life Sciences Biomarker SaveFull Paper
2023Baraibar, I;García, A;Salvà, F;Ros, J;Saoudi, N;Comas, R;Castillo, G;Sanchis, M;García-Álvarez, A;Hernando, J;Capdevila, J;Castells, MR;Martí, M;Landolfi, S;Espín, E;Navalpotro, B;Guevara, J;Dopazo, C;Nuciforo, P;Vivancos, A;Tabernero, J;Élez, E;Impact of the COVID-19 pandemic in the early-onset colorectal cancerTranslational oncology37031602Paolo Nuciforo reports personal financial interest in form of scientific consultancy role for Targos Molecular Pathology. His-institution has received research funding from Save;- Paolo Nuciforo reports personal financial interest in form of scieFull Paper
2023Pousse, L;Korfi, K;Medeiros, BC;Berrera, M;Kumpesa, N;Eckmann, J;Hutter, IK;Griesser, V;Karanikas, V;Klein, C;Amann, M;CD25 targeting with the afucosylated human IgG1 antibody RG6292 eliminates regulatory T cells and CD25+ blasts in acute myeloid leukemiaFrontiers in oncology37205201Frozen healthy BMMC were purchased from Discovery Life Sciences. All human samples were collected under the approval of the relevant Institutional Review Board (IRB SaveFull Paper
2023Kourula, S;Derksen, M;Jardi, F;Jonkers, S;van Heerden, M;Verboven, P;Theuns, V;Van Asten, S;Huybrechts, T;Kunze, A;Frazer-Mendelewska, E;Lai, KW;Overmeer, R;Roos, JL;Vries, RGJ;Boj, SF;Monshouwer, M;Pourfarzad, F;Snoeys, J;Intestinal organoids as an in vitro platform to characterize disposition, metabolism, and safety profile of small moleculesEuropean journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences37244450Intestinal organoids derived from LGR5 + adult stem cells allow for long-term culturing, more closely resemble human physiology than traditional intestinal models, like Save;- HQM Incubation Medium and co-factor N were acquired from in vitro ADMEFull Paper
2023Gnanapragasam, MN;Planutis, A;Glassberg, JA;Bieker, JJ;Identification of a genomic DNA sequence that quantitatively modulates KLF1 transcription factor expression in differentiating human hematopoietic cellsScientific reports37165057is human GRCh37/hg19 genome browser (https://main.genome-browser.bx.psu.edu/index.html), focusing on results using CD34+ or bone marrow derived erythroid cells. Human CD34+ cells were purchased from AllCells or obtained from the Yale Cooperative CenFull Paper
2023Li, J;Zhang, Z;Zhuang, Y;Wang, F;Cai, T;Small RNA transcriptome analysis using parallel single-cell small RNA sequencingScientific reports37160973ng). PBMCs preparation Cryopreserved CD3+ Pan T cells (PB009-1F-C-5M), CD19+ B cells (PB010-P-F-C), CD14+ monocytes (PB-011-P-F-1-C), and CD56+ natural killer cells (PB012-P-F-C) were purchased from AllCells (Shanghai, China). The CD3+ Pan T cells wFull Paper
2023Ke, H;Zhang, F;Wang, J;Xiong, L;An, X;Tu, X;Chen, C;Wang, Y;Mao, B;Guo, S;Ju, C;He, X;Sun, R;Zhang, L;O'Connor, OA;Li, QX;HX009, a novel BsAb dual targeting PD1 x CD47, demonstrates potent anti-lymphoma activity in preclinical modelsScientific reports37012357Gibco). Jurkat-CSR cells, from ImmuneOnco Biopharmaceuticals (Shanghai), were cultured in RPMI-1640 (Gibco) with 10% FBS (Gemini). Human PBMCs used in the studies were purchased from ALLCELL (https://allcells.com) where informed consent and approvedFull Paper
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2023Sweeney, EE;Sekhri, P;Telaraja, D;Chen, J;Chin, SJ;Chiappinelli, KB;Sanchez, CE;Bollard, CM;Cruz, CRY;Fernandes, R;Engineered tumor-specific T cells using immunostimulatory photothermal nanoparticlesCytotherapy37278683ix-coated plates in Astrocyte Medium (Thermo Fisher Scientific). Cells were expanded as per the specifications provided by the supplier. Healthy donor peripheral blood leukopaks were purchased from AllCells (Alameda, CA, USA) with the corresponding hFull Paper
2023Zhang, L;Yu, L;Xu, Y;Qin, P;Shen, P;Liu, K;Fei, M;Wang, H;Cao, Y;Lu, L;Gao, W;Zhang, Z;Demonstrating analytical similarity of a biosimilar HLX04 to bevacizumab with a panel of state-of-the-art methods and tiering of quality attributesAnalytical and bioanalytical chemistry37162525Human umbilical vein endothelial cell (HUVEC), obtained from Allcells (No. H-001F, CA, USA), was used to determine the inhibitory effects of HLX04 and CN-/EU-Avastin® on proliferation of cells expressing VEGFR. HUVEC expresses VEGFR that binds to VEGFull Paper
2023Verner, EL;Jackson, JB;Severson, E;Valkenburg, KC;Greer, AE;Riley, DR;Sausen, M;Maddox, C;McGregor, PM;Karandikar, A;Hastings, SB;Previs, RA;Reddy, VP;Jensen, TJ;Ramkissoon, SH;Validation of the Labcorp Plasma Focus Test to Facilitate Precision Oncology Through Cell-Free DNA Genomic Profiling of Solid TumorsThe Journal of molecular diagnostics : JMD37068734Clinical samples used to evaluate the analytical performance of the Labcorp Plasma Focus test were sourced from multiple biorepositories or as part of internal sample collections (BioIVT, Chestertown, MD; Discovery Life Sciences, Huntsville, AL; PrecFull Paper
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2023Ekiciler, A;Chen, WLK;Bo, Y;Pugliano, A;Donzelli, M;Parrott, N;Umehara, K;Quantitative Cytochrome P450 3A4 Induction Risk Assessment Using Human Hepatocytes Complemented with Pregnane X Receptor-Activating ProfilesDrug metabolism and disposition: the biological fate of chemicals36460477on 24- or 96-well collagen I-coated plates at 0.6 or 0.06 × 10 6 cells/well, respectively, using high viability cryo-hepatocyte recovery kits (In Vitro ADMET Laboratories, LLC; SaveFull Paper
2023Bell-Glenn, S;Salas, LA;Molinaro, AM;Butler, RA;Christensen, BC;Kelsey, KT;Wiencke, JK;Koestler, DC;Calculating detection limits and uncertainty of reference-based deconvolution of whole-blood DNA methylation dataEpigenomics37337720Cells used to isolate the DNA to create the mixtures were purchased from AllCells corporation (CA, USA), StemExpress (CA, USA), and STEMCELL Technologies ( SaveFull Paper
2023Banerjee, P;Diniz, WJS;Rodning, SP;Dyce, PW;miRNA expression profiles of peripheral white blood cells from beef heifers with varying reproductive potentialFrontiers in genetics37234872The quality-checked libraries were pooled and sequenced in the NextSeq 500 using the single-end 50 bp chemistry at Discovery Life Sciences (Hudson Alpha Institute of Save;nt Bioanalyzer high-sensitivity DNA assay (Agilent, Santa Clara, CA, United SFull Paper
2023Lamothe, RC;Storlie, MD;Espinosa, DA;Rudlaff, R;Browne, P;Liu, J;Rivas, A;Devoto, A;Oki, J;Khoubyari, A;Goltsman, DSA;Lin, JL;Butterfield, CN;Brown, CT;Thomas, BC;Cost, GJ;Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome EngineeringThe CRISPR journal37219969oporation, cells were harvested for flow cytometry and genomic DNA extraction as described previously. Primary HSC culture and nucleofection Mobilized peripheral blood CD34+ cells were acquired from AllCells and cultured in StemCell StemSpan SFEM IFull Paper
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2023Li, X;Le, Y;Seo, JE;Guo, X;Li, Y;Chen, S;Mittelstaedt, RA;Moore, N;Guerrero, S;Sims, A;King, ST;Atrakchi, AH;McGovern, TJ;Davis-Bruno, KL;Keire, DA;Elespuru, RK;Heflich, RH;Mei, N;Revisiting the mutagenicity and genotoxicity of N-nitroso propranolol in bacterial and human in vitro assaysRegulatory toxicology and pharmacology : RTP37210026National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, 72079, USA. Electronic address: xilin.li@fda.hhs.gov.++National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, 72079,Full Paper
2023Coleman, R;Metastasis prevention with bone-targeted agents: a complex interaction between the microenvironment and tumour biologyJournal of bone and mineral metabolism37162605amplification status was blinded and followed pre-specified sample handling and standard operating methodology by a central laboratory (Targos Molecular Pathology, SaveFull Paper
2023Kliver, S;Houck, ML;Perelman, PL;Totikov, A;Tomarovsky, A;Dudchenko, O;Omer, AD;Colaric, Z;Weisz, D;Aiden, EL;Chan, S;Hastie, A;Komissarov, A;Ryder, OA;Graphodatsky, A;Johnson, WE;Maldonado, JE;Pukazhenthi, BS;Marinari, PE;Wildt, DE;Koepfli, KP;Chromosome-length genome assembly and karyotype of the endangered black-footed ferret (Mustela nigripes)The Journal of heredity37249392The black-footed ferret (Mustela nigripes) narrowly avoided extinction to become an oft-cited example of the benefits of intensive management, research, and collaboration SaveFull Paper
2023Martha, L;Nakata, A;Furuya, S;Liu, W;Zhang, X;Mizoi, K;Ogihara, T;Transporter and metabolic enzyme-mediated intra-enteric circulation of SN-38, an active metabolite of irinotecan: A new conceptBiochemical and biophysical research communications37148742sages 72-83 were used. Human cryopreserved suspension enterocytes (Lot. HE3072, male, 55 years old, Caucasian) were purchased from In Vitro ADMET Laboratories (MD, USA). All other reagents and solvents were commercial products of special gradeFull Paper
2023He, S;Liu, J;Hu, L;Zhan, Y;Tong, H;Zhu, H;Guo, H;Sun, H;Liu, M;Design, synthesis, biological evaluation and molecular docking studies of quercetin-linker-H2S donor conjugates for the treatment of diabetes and wound healingChemistry & biodiversity37329234All cell culture materials were obtained from Gibco. HepG2 cells were cryopreserved in our laboratory and used after resuscitation. HUVECs cells were purchased from Shanghai Allcells Co., LtdFull Paper
2023Thompson, EN;Carlino, MJ;Scanlon, VM;Grimes, HL;Krause, DS;Assay optimization for the objective quantification of human multilineage colony-forming unitsExperimental hematology37271449Cells were derived commercially (AllCells, Alameda, CA) or were derived from healthy donors who were already donating cells for allogeneic transplantation provided SaveFull Paper
2023Goda, C;Kolovich, S;Rudich, A;Karunasiri, M;Kulkarni, R;Rajgolikar, G;Neidemire-Colley, L;Singh, S;Sircar, A;Ranganathan, P;Garzon, R;Sehgal, L;Dorrance, AM;Epidermal growth factor-like 7 is a novel therapeutic target in mantle cell lymphomaExperimental hematology37209901Full Paper
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2023Chen, Z;Javed, N;Moore, M;Wu, J;Sun, G;Vinyard, M;Collins, A;Pinello, L;Najm, FJ;Bernstein, BE;Integrative dissection of gene regulatory elements at base resolutionCell genomics37388913us Biologicals Cat#NB100-1800 TAL1 antibody SantaCruz Biotech Cat#sc-393287 Cell lines and primary cells Jurkat cell line, Clone E6.1 ATCC Cat#TIB152, RRID:CVCL_0367 CD4+ T cells AllCells N/A ------------------------- ChemicalFull Paper
2023Kessler, ER;Callihan, E;Hu, J;Eule, C;Srivastava, G;Kemme, DJ;Iruku, P;Rana, V;Moore, J;Schuster, SR;Amirault, M;Flaig, TW;Lam, ET;A Phase I/II Clinical Trial of Pembrolizumab and Cabozantinib in Metastatic Renal Cell CarcinomaCancer research communications37377613Full Paper
2023Farabaugh, CS;Doak, S;Roy, S;Elespuru, R;In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin BFrontiers in toxicology37180488Urbana?Champaign, Champaign, United States Khaled Habas, University of Bradford, United Kingdom *Correspondence: Rosalie Elespuru, rosalie.elespuru@dls.com † Present address: Rosalie Elespuru, Discovery Life Sciences, Columbia, MD, United StatesFull Paper
2023Baro-Sastre, B;Kim, CY;Lin, C;Kongsomboonvech, AK;Tetard, M;Salinas, ND;Tolia, NH;Egan, ES;Plasmodium falciparum exploits CD44 as a co-receptor for erythrocyte invasionbioRxiv : the preprint server for biology37090581Generation of cRBCs was performed essentially as previously described 37. Briefly, bone marrow primary human CD34+ HSPCs (Stem Cell Technologies; AllCells) were cultured in PIMDM composed of Iscove Basal Medium (Sigma) with 4 mM L-Glutamine, 330 µg/mFull Paper
2023Okano, F;Saito, T;Minamida, Y;Kobayashi, S;Ido, T;Miyauchi, Y;Wasai, U;Akazawa, D;Kume, M;Ishibashi, M;Jiang, K;Aicher, A;Heeschen, C;Yonehara, T;Identification of Membrane-expressed CAPRIN-1 as a Novel and Universal Cancer Target, and Generation of a Therapeutic Anti-CAPRIN-1 Antibody TRK-950Cancer research communications37082579ng formula based on the Cr-51 dose released from the target cells: The ADCC activity of TRK-950 via human and monkey PBMCs was conducted at Translational Drug Development (TD2). We used human PBMCs (AllCells, catalog no. PB003F) and Cynomolgus MonkeFull Paper
2023Montalban-Bravo, G;Ma, F;Thongon, N;Yang, H;Gomez, IG;Rodriguez-Sevilla, JJ;Adema, V;Wildeman, B;Lockyer, P;Kim, YJ;Tanaka, T;Darbaniyan, F;Pancholy, S;Zhang, G;Al-Atrash, G;Dwyer, K;Takahashi, K;Garcia-Manero, G;Kantarjian, H;Colla, S;Targeting MCL1-driven anti-apoptotic pathways to overcome hypomethylating agent resistance in RAS -mutated chronic myelomonocytic leukemiabioRxiv : the preprint server for biology37066354RAS pathway mutations, which are present in 30% of patients with chronic myelomonocytic leukemia (CMML) at diagnosis, confer a high risk of resistance to and SaveFull Paper
2023Dillon, LW;Higgins, J;Nasif, H;Othus, M;Beppu, L;Smith, TH;Schmidt, E;Valentine, CC;Salk, JJ;Wood, BL;Erba, HP;Radich, JP;Hourigan, CS;Quantification of measurable residual disease using duplex sequencing in adults with acute myeloid leukemiamedRxiv : the preprint server for health sciences37034683The presence of measurable residual disease (MRD) is strongly associated with treatment outcomes in acute myeloid leukemia (AML). Despite the correlation with clinical SaveFull Paper
2023Flümann, R;Hansen, J;Pelzer, BW;Nieper, P;Lohmann, T;Kisis, I;Riet, T;Kohlhas, V;Nguyen, PH;Peifer, M;Abedpour, N;Bosco, G;Thomas, RK;Kochanek, M;Knüfer, J;Jonigkeit, L;Beleggia, F;Holzem, A;Büttner, R;Lohneis, P;Meinel, J;Ortmann, M;Persigehl, T;Hallek, M;Calado, DP;Chmielewski, M;Klein, S;Göthert, JR;Chapuy, B;Zevnik, B;Wunderlich, FT;von Tresckow, B;Jachimowicz, RD;Melnick, AM;Reinhardt, HC;Knittel, G;Distinct Genetically Determined Origins of Myd88/BCL2-Driven Aggressive Lymphoma Rationalize Targeted Therapeutic Intervention StrategiesBlood cancer discovery36346827Roche, Cofounder and CSO of Targos MP Inc. (until May 2021), Gnothis Inc/Stockholm (Member of Board of Directors and Coowner). D.P. Calado reports other support from Cancer Research UK (CC2078), Wellcome Trust (CC2078), and UK Medical Research CounciFull Paper
2023Mark W., R;Joanne, W;Mary Jo, K;Melissa E., M;John V., L;Peter, F;Sleep Disturbances and Chronic Pain as Potential Indicators for Therapeutic Intervention in Subjects with Post-Traumatic Stress DisorderJournal of Depression and Anxiety Disorderscruited to an age and gender-matched case-control study (N = 78; control n = 39; PTSD n = 39). Participants were recruited in the US between January and June 2019 by PrecisionMed (California, US) and Discovery Life Sciences (California, US). A compreFull Paper
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2023Ridzuan, A;Sebastian, A;Noorazman, A;Kannan, T;Syed, N;Nurul, A;Interleukin-17A enhances osteogenic differentiation by activating ERK/MAPK in stem cells derived from human exfoliated deciduous teethBiomedical Research and Therapythe participation of the MAPK signaling pathway in SHED osteogenic differentiation. MATERIALS AND METHODS CELL CULTURE AND OSTEOGENIC DIFFERENTIATION IN VITRO The SHED cell line, obtained from ALLCELLS (Alameda, CA, USA), was derived from humaFull Paper
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2023Kessler, E;Callihan, E;Hu, J;Eule, C;Srivastava, G;Kemme, D;Iruku, P;Rana, V;Moore, J;Schuster, S;Amirault, M;Flaig, T;Lam, E;Data from A Phase I/II Clinical Trial of Pembrolizumab and Cabozantinib in Metastatic Renal Cell CarcinomaAmerican Association for Cancer ResearchPD-L1 testing was performed on archival tumor tissues by Discovery Life Sciences (formerly QualTek Molecular Laboratories) using a previously validated IHC assay for SaveFull Paper
2023Sharma, P;Zhang, X;Ly, K;Kim, J;Wan, Q;Kim, J;Lou, M;Kain, L;Teyton, L;Winau, F;Hyperglycosylation of prosaposin in tumor DCs promotes immune escape in cancerbioRxivDissociated whole tumor samples were purchased from Discovery Life Sciences (USA). Melanoma, myeloid, and CD8 T cells were FACS-sorted as described above. Melanoma cells were ?-irradiated (10,000 rad), and induction of apoptosis wasFull Paper
2023Tierney, B;Foox, J;Ryon, K;Butler, D;Damle, N;Young, B;Mozsary, C;Babler, K;Yin, X;Carattini, Y;Andrews, D;Solle, N;Kumar, N;Shukla, B;Vidovic, D;Currall, B;Williams, S;Schürer, S;Stevenson, M;Amirali, A;Beaver, C;Kobetz, E;Boone, M;Reding, B;Laine, J;Comerford, S;Lamar, W;Tallon, J;Hirschberg, J;Proszynski, J;Sharkey, M;Church, G;Grills, G;Solo-Gabriele, H;Mason, C;Geospatially-resolved public-health surveillance via wastewater sequencingmedRxiv550 HudsonAlpha Discovery (Huntsville, AL) performed all sequencing library preparation on the 551 generated nucleic acids. The nucleic acid samples underwent SaveFull Paper
2023Zhang, X;Meng, F;Lyu, W;He, J;Wei, R;Du, Z;Zhang, C;Guan, Y;Huang, X;Lyu, G;Tian, X;Zhang, L;Tao, W;Histone lactylation antagonizes senescence and skeletal muscle aging via facilitating gene expression reprogrammingbioRxivIMR90 cells were purchased from the American Type Culture Collection (ATCC), HUVECs were purchased from AllCells, and MEF cells were isolated from 12.5-14- day-old embryos of C57BL/6 miceFull Paper
2023Sorensen, JT;Classification of a novel subgroup of shared hepatitis B virus core and e antigen epitopes and their antibody reactivity in clinical immunoassay formatsThesisProtocols for BSL-2 ELISA are summarized in section 5.3.1. HBV positive samples were all purchased from Discovery Life Sciences Biological Sample RepositoryFull Paper
2023Najia, MA;Transposable elements and the regulatory logic of hematopoietic differentiationThesisumbilical cord blood cells (AllCells) were thawed from liquid nitrogen storage via CD34+ umbilical cord blood cells (AllCells) were thawed from liquid nitrogen storage and SaveFull Paper
2023Schejtman Borisonik, A;Lentiviral gene therapy for p47-deficient Chronic Granulomatous diseaseThesisCD34+ cells used in the experiments were frozen vials of previously isolated HD CD34+ cells from a G-CSF mobilised apheresis (ALLCells) using the CliniMACS prodigyFull Paper
2023Kortchak, S;Development of Programmed Cell Death Ligand Derived Peptides for the Treatment of Autoimmune DiseasesThesisCell Culture. Jurkat NFAT-PD-1 reporter cells (BPS Biosciences, San Diego, CA) were maintained in Growth Medium 2A (BPS Biosciences, San Diego, CA) medium. Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells and maintained in RPMIFull Paper
2023Maughon Jr, TS;Metabolic Profiling of Mesenchymal Stromal Cells for Critical Quality Attribute IdentificationThesisat three densities (10,000, 5,000 and 2,000 cells/well) in a 96 well plate and cultured for 24 hours. PBMCs (AllCells, Alameda CA) were thawed in Roswell Park Memorial Institute (RPMI) media (RPMI, 20% FBS, 2mM L-glutamine, 50 U/mL penicillin, 50 ?g/Full Paper
2023Han, L;Dong, L;Leung, K;Zhao, Z;Li, Y;Gao, L;Chen, Z;Xue, J;Qing, Y;Li, W;Pokharel, SP;Gao, M;Chen, M;Shen, C;Tan, B;Small, A;Wang, K;Zhang, Z;Qin, X;Yang, L;Wunderlich, M;Zhang, B;Mulloy, JC;Marcucci, G;Chen, CW;Wei, M;Su, R;Chen, J;Deng, X;METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolismCell stem cell36608679ColonyGEL-Mouse Base Medium Reachbio Cat# 1201 ColonyGEL -Human Base Medium Reachbio Cat# 1101Full Paper
2023Le Floc'h, A;Nagashima, K;Birchard, D;Scott, G;Ben, LH;Ajithdoss, D;Gayvert, K;Romero Hernandez, A;Herbin, O;Tay, A;Farrales, P;Korgaonkar, CK;Pan, H;Shah, S;Kamat, V;Chatterjee, I;Popke, J;Oyejide, A;Lim, WK;Kim, JH;Huang, T;Franklin, M;Olson, W;Norton, T;Perlee, L;Yancopoulos, GD;Murphy, AJ;Sleeman, MA;Orengo, JM;Blocking common γ chain cytokine signaling ameliorates T cell-mediated pathogenesis in disease modelsScience translational medicine36630481To induce GVHD, we injected immunodeficient NSG (Jackson Laboratory, 005557) female mice (8 weeks old) retro-orbitally with 10 7 human PBMCs (ReachBio, 0500-401). Mice were SaveFull Paper
2023Wang, H;Georgakopoulou, A;Zhang, W;Kim, J;Gil, S;Ehrhardt, A;Lieber, A;HDAd6/35++ - A new helper-dependent adenovirus vector platform for in vivo transduction of hematopoietic stem cellsMolecular therapy. Methods & clinical development37081854Lineage-negative (Lin-) cells were isolated by depletion of lineage-committed cells in BM MNCs using the mouse lineage cell depletion kit (Miltenyi Biotec) according to the manufacturer’s instructions. Colony-forming unit assays were performed usingFull Paper
2023Belcher, JD;Nataraja, S;Abdulla, F;Zhang, P;Chen, C;Nguyen, J;Ruan, C;Singh, M;Demes, S;Olson, L;Stickens, D;Stanwix, J;Clarke, E;Huang, Y;Biddle, M;Vercellotti, GM;The BACH1 inhibitor ASP8731 inhibits inflammation and vaso-occlusion and induces fetal hemoglobin in sickle cell diseaseFrontiers in medicine37144034Department of Medicine, Division of Hematology, Oncology and Transplantation, University of Minnesota, Minneapolis, MN, United States.++Mitobridge Inc., Cambridge, MA, United States.++Department of Medicine, Division of Hematology, Oncology and TransFull Paper
2023Wu, T;Pelus, LM;Plett, PA;Sampson, CH;Chua, HL;Fisher, A;Feng, H;Liu, L;Li, H;Ortiz, M;Chittajallu, S;Luo, Q;Bhatwadekar, AD;Meyer, TB;Zhang, X;Zhou, D;Fischer, KD;McKinzie, DL;Miller, SJ;Orschell, CM;Further Characterization of Multi-Organ DEARE and Protection by 16,16 Dimethyl Prostaglandin E2 in a Mouse Model of the Hematopoietic Acute Radiation SyndromeRadiation research37014943Total nucleated BM cells from non-irradiated mice were plated at 2 × 104 per dish in ColonyGEL 1202 Mouse Complete Medium (Reach Bio, Seattle, WA)Full Paper
2023Borot, F;Humbert, O;Newby, GA;Fields, E;Kohli, S;Radtke, S;Laszlo, GS;Mayuranathan, T;Ali, AM;Weiss, MJ;Yen, JS;Walter, RB;Liu, DR;Mukherjee, S;Kiem, HP;Multiplex Base Editing to Protect from CD33-Directed Therapy: Implications for Immune and Gene TherapybioRxiv : the preprint server for biology36865281Cord blood CD34+ 526 cells were 527 additionally cultured with UM171 0.35 nM (Xcessbio). In colony-forming cell assays, 1000-1200 528 sorted cells were seeded into 3.5 ml ColonyGEL 1402 (ReachBio)Full Paper
2023Vujovic, A;de Rooij, L;Chahi, AK;Chen, HT;Yee, BA;Loganathan, SK;Liu, L;Chan, DCH;Tajik, A;Tsao, E;Moreira, S;Joshi, P;Xu, J;Wong, N;Balde, Z;Jahangiri, S;Zandi, S;Aigner, S;Dick, JE;Minden, MD;Schramek, D;Yeo, GW;Hope, KJ;In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic TargetBlood cancer discovery36763002Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio cat. no. 1102), supplemented with 1.1 μmol/L DHTS (MilliporeSigma; cat. no. D0947-10MG) or equivalent DMSO (FishFull Paper
2023Zhang, Z;Zhou, K;Han, L;Small, A;Xue, J;Huang, H;Weng, H;Su, R;Tan, B;Shen, C;Li, W;Zhao, Z;Qing, Y;Qin, X;Wang, K;Leung, K;Boldin, M;Chen, C;Ann, D;Qian, Z;Deng, X;Chen, J;Chen, Z;RNA m6A reader YTHDF2 facilitates precursor miR-126 maturation to promote acute myeloid leukemia progressionGenes & DiseasesColonyGel 1201-mouse base medium (1201, Reachbio) were used for seeding cells at 20, 000 per 35 mm dish. Murine cytokines and reagents were supplemented as follows: 10 ng/ SaveFull Paper
2023Davies, J;Prieto, EM;Guelcher, S;Characterization of Macrophage Behavior in the Human Immunoresponse and Bone Remodelingcdn.vanderbilt.eduWe cultured primary human monocytes (Reachbio, Seattle, WA) in Corning T25 flasks with 10 mL of modified Dulbecco’s modified Eagle medium (DMEM) media and split them every 9 days. We changed media on Day 1, 5, and 7, according to Table 1. PenicillinFull Paper
2023Vujovic, A;Investigating RNA-Binding Protein Dependencies in Hematopoietic and Acute Myeloid Leukemic Stem CellsThesisThawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio Cat# 1102), supplemented with 1.1μM DHTS (MilliporeSigma Cat# D0947-10MG) or equivalent DMSO (Fisher ScientificFull Paper
2022Somarathna, M;Hwang, PT;Millican, RC;Alexander, GC;Isayeva-Waldrop, T;Sherwood, JA;Brott, BC;Falzon, I;Northrup, H;Shiu, YT;Stubben, CJ;Totenhagen, J;Jun, HW;Lee, T;Nitric oxide releasing nanomatrix gel treatment inhibits venous intimal hyperplasia and improves vascular remodeling in a rodent arteriovenous fistulaBiomaterials34836683RNA isolation and sequencing were performed by Discovery Life Sciences (Huntsville, AL). Total RNA was isolated using standard Discovery Life Sciences protocols. All samples underwent RNA quality control assessment using a Fragment Analyzer (AgilenFull Paper
2022Choi, DY;Park, JN;Paek, SH;Choi, SC;Paek, SH;Detecting early-stage malignant melanoma using a calcium switch-enriched exosome subpopulation containing tumor markers as a sampleBiosensors & bioelectronics34847362Clinical samples of human sera from healthy donors and malignant melanoma patients were obtained from Discovery Life Sciences (DLS; Los Osos, CA, USA). Other materials are listed and described about the sources in details in Supplementary InformatiFull Paper
2022Bormann Chung, C;Lee, J;Barritault, M;Bringuier, PP;Xu, Z;Huang, WY;Beharry, A;Castillo, J;Christiansen, J;Lin, JC;Sheffield, BS;Evaluating Targeted Next-Generation Sequencing Assays and Reference Materials for NTRK Fusion DetectionThe Journal of molecular diagnostics : JMD34656759The 30 FFPE NTRK fusion-negative tumor samples (confirmed as NTRK fusion negative by an NGS laboratory-developed test) tested in the specificity analyses for AFL, TSO500, and OFA were purchased from Indivumed GmbH (Hamburg, Germany) and Discovery LifFull Paper
2022Freeman, J;Conklin, J;Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference materialJournal of virological methods34915088WHO 20/136 is standard reference material for SARS-CoV-2 serology assays. Standardization of serology assays that target the same antigen and class of immunoglobulin will enable comparison of results between studies that use various lab-developed aFull Paper
2022Ganan-Gomez, I;Yang, H;Ma, F;Montalban-Bravo, G;Thongon, N;Marchica, V;Richard-Carpentier, G;Chien, K;Manyam, G;Wang, F;Alfonso, A;Chen, S;Class, C;Kanagal-Shamanna, R;Ingram, JP;Ogoti, Y;Rose, A;Loghavi, S;Lockyer, P;Cambo, B;Muftuoglu, M;Schneider, S;Adema, V;McLellan, M;Garza, J;Marchesini, M;Giuliani, N;Pellegrini, M;Wang, J;Walker, J;Li, Z;Takahashi, K;Leverson, JD;Bueso-Ramos, C;Andreeff, M;Clise-Dwyer, K;Garcia-Manero, G;Colla, S;Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapyNature medicine35241842simultaneously received the cytidine deaminase inhibitor E7727 (cedazuridine) to increase HMA bioavailability. BM samples from 21 HDs (median age, 53 years; range, 49-66 years) were obtained from AllCells or from MD Anderson’s Department of Stem CelFull Paper
2022Lebedin, M;Foglierini, M;Khorkova, S;Vázquez García, C;Ratswohl, C;Davydov, AN;Turchaninova, MA;Daubenberger, C;Chudakov, DM;Lanzavecchia, A;de la Rosa, K;Different classes of genomic inserts contribute to human antibody diversityProceedings of the National Academy of Sciences of the United States of America3603735342), and ethical review boards at Ifakara Health Institute and the University of Lausanne, and all volunteers provided informed consent before blood donation. Bone marrow specimens were obtained from AllCells. Extraction of Human PBMCs. Blood sampleFull Paper
2022Simmons, SK;Lithwick-Yanai, G;Adiconis, X;Oberstrass, F;Iremadze, N;Geiger-Schuller, K;Thakore, PI;Frangieh, CJ;Barad, O;Almogy, G;Rozenblatt-Rosen, O;Regev, A;Lipson, D;Levin, JZ;Mostly natural sequencing-by-synthesis for scRNA-seq using Ultima sequencingNature biotechnology36109685We purchased nine cryopreserved human PMBC samples (AllCells). We thawed PBMC vials in a 37 °C water bath for ~2 min. A quick counting revealed high viability (90%) in all samples. We added 1 ml RPMI1640 (Thermo Fisher Scientific, 11875093) with 10%Full Paper
2022Shy, BR;Vykunta, VS;Ha, A;Talbot, A;Roth, TL;Nguyen, DN;Pfeifer, WG;Chen, YY;Blaeschke, F;Shifrut, E;Vedova, S;Mamedov, MR;Chung, JJ;Li, H;Yu, R;Wu, D;Wolf, J;Martin, TG;Castro, CE;Ye, L;Esensten, JH;Eyquem, J;Marson, A;High-yield genome engineering in primary cells using a hybrid ssDNA repair template and small-molecule cocktailsNature biotechnology36008610Primary adult blood cells were obtained from healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc. or Allcells Inc, as a Trima residual from Vitalant, or from fresh whole blood under a protocol approved by the UCSF CFull Paper
2022Zhang, B;Srivastava, A;Mimitou, E;Stuart, T;Raimondi, I;Hao, Y;Smibert, P;Satija, R;Characterizing cellular heterogeneity in chromatin state with scCUT&Tag-proNature biotechnology35332340available on the protocols.io platform, here: https://www.protocols.io/private/57E45A034D5311ECA43B0A58A9FEAC02. PBMC acquisition and processing Cryopreserved healthy donor PBMCs were purchased from AllCells. After thawing into DMEM with 10% FBS, thFull Paper
2022Fiskin, E;Lareau, CA;Ludwig, LS;Eraslan, G;Liu, F;Ring, AM;Xavier, RJ;Regev, A;Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATACNature biotechnology34675424Thermo Fisher Scientific), resuspended in DMEM containing 10% FBS, centrifuged, washed with PBS and resuspended in FC buffer (above). For PBMC and CD8 T-cell experiments, cryopreserved PBMCs or CD8 T cells (AllCells) were thawed, washed in PBS and reFull Paper
2022Omer-Javed, A;Pedrazzani, G;Albano, L;Ghaus, S;Latroche, C;Manzi, M;Ferrari, S;Fiumara, M;Jacob, A;Vavassori, V;Nonis, A;Canarutto, D;Naldini, L;Mobilization-based chemotherapy-free engraftment of gene-edited human hematopoietic stem cellsCell35617958V4XP-3032 TaqMan Gene Expression Assay ThermoFisher Scientifi Cat# 4331182 ------------------------- Experimental models: Cell lines ------------------------- Human: G-CSF mPB CD34+ HSPCs AllCells N/A Human: G-CSF/Mozobil mPB CD34+ HSFull Paper
2022Battaglia, S;Dong, K;Wu, J;Chen, Z;Najm, FJ;Zhang, Y;Moore, MM;Hecht, V;Shoresh, N;Bernstein, BE;Long-range phasing of dynamic, tissue-specific and allele-specific regulatory elementsNature genetics36195755Epigenomic maps identify gene regulatory elements by their chromatin state. However, prevailing short-read sequencing methods cannot effectively distinguish alleles, evaluate the SaveFull Paper
2022Bottomly, D;Long, N;Schultz, AR;Kurtz, SE;Tognon, CE;Johnson, K;Abel, M;Agarwal, A;Avaylon, S;Benton, E;Blucher, A;Borate, U;Braun, TP;Brown, J;Bryant, J;Burke, R;Carlos, A;Chang, BH;Cho, HJ;Christy, S;Coblentz, C;Cohen, AM;d'Almeida, A;Cook, R;Danilov, A;Dao, KT;Degnin, M;Dibb, J;Eide, CA;English, I;Hagler, S;Harrelson, H;Henson, R;Ho, H;Joshi, SK;Junio, B;Kaempf, A;Kosaka, Y;Laderas, T;Lawhead, M;Lee, H;Leonard, JT;Lin, C;Lind, EF;Liu, SQ;Lo, P;Loriaux, MM;Luty, S;Maxson, JE;Macey, T;Martinez, J;Minnier, J;Monteblanco, A;Mori, M;Morrow, Q;Nelson, D;Ramsdill, J;Rofelty, A;Rogers, A;Romine, KA;Ryabinin, P;Saultz, JN;Sampson, DA;Savage, SL;Schuff, R;Searles, R;Smith, RL;Spurgeon, SE;Sweeney, T;Swords, RT;Thapa, A;Thiel-Klare, K;Traer, E;Wagner, J;Wilmot, B;Wolf, J;Wu, G;Yates, A;Zhang, H;Cogle, CR;Collins, RH;Deininger, MW;Hourigan, CS;Jordan, CT;Lin, TL;Martinez, ME;Pallapati, RR;Pollyea, DA;Pomicter, AD;Watts, JM;Weir, SJ;Druker, BJ;McWeeney, SK;Tyner, JW;Integrative analysis of drug response and clinical outcome in acute myeloid leukemiaCancer cell35868306Acute myeloid leukemia (AML) is a cancer of myeloid-lineage cells with limited therapeutic options. We previously combined ex vivo drug sensitivity with genomic, transcriptomic, and SaveFull Paper
2022Ma, H;Guo, Y;Tang, H;Tseng, CK;Wang, L;Zong, H;Wang, Z;He, Y;Chang, Y;Wang, S;Huang, H;Ke, Y;Yuan, Y;Wu, M;Zhang, Y;Drelich, A;Kempaiah, KR;Peng, BH;Wang, A;Yang, K;Yin, H;Liu, J;Yue, Y;Xu, W;Zhu, S;Ji, T;Zhang, X;Wang, Z;Li, G;Liu, G;Song, J;Mu, L;Xiang, Z;Song, Z;Chen, H;Bian, Y;Zhang, B;Chen, H;Zhang, J;Liao, Y;Zhang, L;Yang, L;Chen, Y;Gilly, J;Xiao, X;Han, L;Jiang, H;Xie, Y;Zhou, Q;Zhu, J;Broad ultra-potent neutralization of SARS-CoV-2 variants by monoclonal antibodies specific to the tip of RBDCell discovery35169121In ADCP experiment, CD14 + monocytes (Allcells) were cultured and differentiated for 7 days to obtain macrophage cells. Macrophages were labeled with violet dye (ThermoFisher), SaveFull Paper
2022Ferrari, S;Jacob, A;Cesana, D;Laugel, M;Beretta, S;Varesi, A;Unali, G;Conti, A;Canarutto, D;Albano, L;Calabria, A;Vavassori, V;Cipriani, C;Castiello, MC;Esposito, S;Brombin, C;Cugnata, F;Adjali, O;Ayuso, E;Merelli, I;Villa, A;Di Micco, R;Kajaste-Rudnitski, A;Montini, E;Penaud-Budloo, M;Naldini, L;Choice of template delivery mitigates the genotoxic risk and adverse impact of editing in human hematopoietic stem cellsCell stem cell36206730tain Kit Thermo Fisher Cat# L34957 7-aminoactinomycin D Sigma Aldrich SML1633; CAS: 7240-37-1 ------------------------- Biological samples ------------------------- Mobilized Leukopak AllCells N/A ------------------------- ChemicaFull Paper
2022Chan, S;Belmar, N;Ho, S;Rogers, B;Stickler, M;Graham, M;Lee, E;Tran, N;Zhang, D;Gupta, P;Sho, M;MacDonough, T;Woolley, A;Kim, H;Zhang, H;Liu, W;Zheng, P;Dezso, Z;Halliwill, K;Ceccarelli, M;Rhodes, S;Thakur, A;Forsyth, CM;Xiong, M;Tan, SS;Iyer, R;Lake, M;Digiammarino, E;Zhou, L;Bigelow, L;Longenecker, K;Judge, RA;Liu, C;Trumble, M;Remis, JP;Fox, M;Cairns, B;Akamatsu, Y;Hollenbaugh, D;Harding, F;Alvarez, HM;An anti-PD-1-GITR-L bispecific agonist induces GITR clustering-mediated T cell activation for cancer immunotherapyNature cancer35256819Human allogeneic PBMCs (AllCells) were used to purify negatively selected populations of T cells (catalog no. 19051, Stemcell) and CD11b monocytes (catalog no. 19058, Stemcell). SaveFull Paper
2022Dey, M;Kim, M;Dogan, M;Nagamine, M;Kozhaya, L;Celik, N;Unutmaz, D;Ozbolat, I;Chemotherapeutics and CAR‐T Cell‐Based Immunotherapeutics Screening on a 3D Bioprinted Vascularized Breast Tumor ModelAdvanced Functional MaterialsLeukopaks from healthy adults were purchased from ALLCells (Alameda, CA) in deidentified form and processed to isolate Peripheral Blood Mononuclear Cells (PBMCs) using Ficoll-paque plus (GE Healthcare). CD8+ T cells were purified from PBMCs using DynFull Paper
2022Elagib, KE;Brock, A;Clementelli, CM;Mosoyan, G;Delehanty, LL;Sahu, RK;Pacheco-Benichou, A;Fruit, C;Besson, T;Morris, SW;Eto, K;Jobaliya, C;French, DL;Gadue, P;Singh, S;Shi, X;Qin, F;Cornelison, R;Li, H;Iancu-Rubin, C;Goldfarb, AN;Relieving DYRK1A repression of MKL1 confers an adult-like phenotype to human infantile megakaryocytesThe Journal of clinical investigation35925681alculator/hypergeometric.aspx). A P value less than 0.05 was considered significant. Study approval. CD34+ cells purchased from the Fred Hutchinson Cancer Research Center (Seattle, Washington, USA), AllCells (Alameda, California, USA), and StemCellFull Paper
2022Brady, JM;Phelps, M;MacDonald, SW;Lam, EC;Nitido, A;Parsons, D;Boutros, CL;Deal, CE;Garcia-Beltran, WF;Tanno, S;Natarajan, H;Ackerman, ME;Vrbanac, VD;Balazs, AB;Antibody-mediated prevention of vaginal HIV transmission is dictated by IgG subclass in humanized miceScience translational medicine35895834To generate huPBMC-engrafted NSG mice, frozen huPBMCs (AllCells) were thawed and expanded in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% fetal bovine serum ( SaveFull Paper
2022Jennings, D;Huntwork-Rodriguez, S;Henry, AG;Sasaki, JC;Meisner, R;Diaz, D;Solanoy, H;Wang, X;Negrou, E;Bondar, VV;Ghosh, R;Maloney, MT;Propson, NE;Zhu, Y;Maciuca, RD;Harris, L;Kay, A;LeWitt, P;King, TA;Kern, D;Ellenbogen, A;Goodman, I;Siderowf, A;Aldred, J;Omidvar, O;Masoud, ST;Davis, SS;Arguello, A;Estrada, AA;de Vicente, J;Sweeney, ZK;Astarita, G;Borin, MT;Wong, BK;Wong, H;Nguyen, H;Scearce-Levie, K;Ho, C;Troyer, MD;Preclinical and clinical evaluation of the LRRK2 inhibitor DNL201 for Parkinson's diseaseScience translational medicine356754332017 {study IDs: SAN-BB-01 [approved by Quorum Review independent review board (IRB)], PLMDEN201609v1 (approved by New England IRB), and US-IRB-13-2016.1 (approved by E&I Review Services)}. PBMCs from two donors for pS935 LRRK2 and pT73 Rab10 dose-reFull Paper
2022Barreyro, L;Sampson, AM;Ishikawa, C;Hueneman, KM;Choi, K;Pujato, MA;Chutipongtanate, S;Wyder, M;Haffey, WD;O'Brien, E;Wunderlich, M;Ramesh, V;Kolb, EM;Meydan, C;Neelamraju, Y;Bolanos, LC;Christie, S;Smith, MA;Niederkorn, M;Muto, T;Kesari, S;Garrett-Bakelman, FE;Bartholdy, B;Will, B;Weirauch, MT;Mulloy, JC;Gul, Z;Medlin, S;Kovall, RA;Melnick, AM;Perentesis, JP;Greis, KD;Nurmemmedov, E;Seibel, WL;Starczynowski, DT;Blocking UBE2N abrogates oncogenic immune signaling in acute myeloid leukemiaScience translational medicine35263148BM samples directly from patients and sequenced. Healthy BM specimens were obtained from ALLCells Inc. Human CD34+ umbilical cord blood and adult BM-derived mononuclear cells were obtained from the Translational Research Development Support LaboratorFull Paper
2022Sandberg, ML;Wang, X;Martin, AD;Nampe, DP;Gabrelow, GB;Li, CZ;McElvain, ME;Lee, WH;Shafaattalab, S;Martire, S;Fisher, FA;Ando, Y;Liu, E;Ju, D;Wong, LM;Xu, H;Kamb, A;A carcinoembryonic antigen-specific cell therapy selectively targets tumor cells with HLA loss of heterozygosity in vitro and in vivoScience translational medicine35235342Informed consent for primary T cells and donor collection protocols were approved by an Institutional Review Board at AllCells or HemaCare. Peripheral blood mononuclear cells (PBMCs) were purified from Leukopaks purchased from AllCells or HemaCare, aFull Paper
2022Uehara, K;Harumoto, T;Makino, A;Koda, Y;Iwano, J;Suzuki, Y;Tanigawa, M;Iwai, H;Asano, K;Kurihara, K;Hamaguchi, A;Kodaira, H;Atsumi, T;Yamada, Y;Tomizuka, K;Targeted delivery to macrophages and dendritic cells by chemically modified mannose ligand-conjugated siRNANucleic acids research35524566are). Preparation of human monocyte-derived macrophages and DCs Two types of human monocyte-derived macrophages (h-Mo-Mφs) were generated as described in Supplementary Figure S1A. CD14+ monocytes (AllCells, Alameda, CA, USA) were cultured (37°C, 5%Full Paper
2022Pennycuick, A;Selway, H;Lam, J;Khan, K;331P Predicting outcomes following colorectal cancer resection: Using real-world data to empower adjuvant treatment decision makingAnnals of Oncology: Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. A. Vivancos: Financial Interests, PersoFull Paper
2022Glaser, M;von Levetzow, C;Michels, S;Nogova, L;Katzenmeier, M;Wömpner, C;Schmitz, J;Bitter, E;Terjung, I;Passmann, E;Schaufler, D;Eisert, A;Fischer, R;Riedel, R;Weber, J;Hahne, S;Merkelbach-Bruse, S;Büttner, R;Wolf, J;Scheffler, M;1729P The biological effect of small-scale ROS1 aberrations: An in silico analysisAnnals of OncologyMS. R. Riedel: Financial Interests, Personal, Funding: Boehringer Ingelheim, Loxo Oncology, Novartis, Lilly. J. Weber: Financial Interests, Personal, Advisory Board: Lilly. R. Büttner: Financial Interests, Personal, Ownership Interest: Targos MoleculFull Paper
2022Poole, A;Karuppiah, V;Hartt, A;Haidar, JN;Moureau, S;Dobrzycki, T;Hayes, C;Rowley, C;Dias, J;Harper, S;Barnbrook, K;Hock, M;Coles, C;Yang, W;Aleksic, M;Lin, AB;Robinson, R;Dukes, JD;Liddy, N;Van der Kamp, M;Plowman, GD;Vuidepot, A;Cole, DK;Whale, AD;Chillakuri, C;Therapeutic high affinity T cell receptor targeting a KRASG12D cancer neoantigenNature communications36088370ersberg, Germany). PBMCs were either purchased cryo-frozen from commercial suppliers (Cellular Technology Limited (CTL), OH & Tissue Solutions, Glasgow, UK) or prepared from healthy donor leukopaks (ALLCELLS, CA) by Ficoll density gradient centrifugFull Paper
2022Sano, Y;Azuma, Y;Tsunenari, T;Kayukawa, Y;Shinozuka, J;Fujii, E;Amano, J;Nishito, Y;Maruyama, T;Kinoshita, Y;Sakamoto, Y;Yoshida, A;Miyazaki, Y;Sato, Y;Teramoto-Seida, C;Ishiguro, T;Tanaka, T;Kitazawa, T;Endo, M;Combination of T cell-redirecting bispecific antibody ERY974 and chemotherapy reciprocally enhances efficacy against non-inflamed tumoursNature communications36071036(Tokyo, Japan). After an acclimatisation of 3 weeks, mice were irradiated (2.5 Gy; MBR-1520R-3; Hitachi Power Solutions Co. Ltd., Ibaraki, Japan) 1 day before transplantation of human CD34+ cells (AllCells, Alameda, CA, USA) via intravenous injectionFull Paper
2022Badrani, JH;Strohm, AN;Lacasa, L;Civello, B;Cavagnero, K;Haung, YA;Amadeo, M;Naji, LH;Lund, SJ;Leng, A;Kim, H;Baum, RE;Khorram, N;Mondal, M;Seumois, G;Pilotte, J;Vanderklish, PW;McGee, HM;Doherty, TA;RNA-binding protein RBM3 intrinsically suppresses lung innate lymphoid cell activation and inflammation partially through CysLT1RNature communications35908044&D Systems, Minneapolis, MN) in T cell media. Post-stimulation supernatant was collected and stored at −80 C for ELISA analysis. Human PBMCs were isolated from commercially purchased leukopacks (Allcells, Alameda, CA, USA). Human peripheral blood ILFull Paper
2022Li, F;Zhang, H;Wang, W;Yang, P;Huang, Y;Zhang, J;Yan, Y;Wang, Y;Ding, X;Liang, J;Qi, X;Li, M;Han, P;Zhang, X;Wang, X;Cao, J;Fu, YX;Yang, X;T cell receptor β-chain-targeting chimeric antigen receptor T cells against T cell malignanciesNature communications35882880ded by Dr. Justin Kline (University of Chicago). B16-OVA cells were kindly provided by Dr. Hans Schreiber (University of Chicago). Human peripheral blood mononuclear cells (PBMCs) were purchased from Allcells Biotechnology, Limited (Shanghai, China)Full Paper
2022Jo, S;Das, S;Williams, A;Chretien, AS;Pagliardini, T;Le Roy, A;Fernandez, JP;Le Clerre, D;Jahangiri, B;Chion-Sotinel, I;Rozlan, S;Dessez, E;Gouble, A;Dusséaux, M;Galetto, R;Duclert, A;Marcenaro, E;Devillier, R;Olive, D;Duchateau, P;Poirot, L;Valton, J;Endowing universal CAR T-cell with immune-evasive properties using TALEN-gene editingNature communications35773273cell products and to allow their large-scale utilization against multiple malignancies for the benefit of a broader range of patients. Methods Materials Cryopreserved human PBMCs were acquired from ALLCELLS (cat# PB006F). PBMCs were cultured in CTSFull Paper
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2022Salas, LA;Zhang, Z;Koestler, DC;Butler, RA;Hansen, HM;Molinaro, AM;Wiencke, JK;Kelsey, KT;Christensen, BC;Enhanced cell deconvolution of peripheral blood using DNA methylation for high-resolution immune profilingNature communications35140201lymphocytes memory (CD4mem), T regulatory cells (Treg), T-cytotoxic lymphocytes naïve (CD8nv), T-cytotoxic lymphocytes memory (CD8mem), and natural killer lymphocytes (NK) cells] were purchased from AllCells corporation (Alameda, CA, USA), StemExpreFull Paper
2022Wiesweg, M;Hense, J;Darwiche, K;Michels, S;Hautzel, H;Kobe, C;Metzenmacher, M;Herold, T;Zaun, G;Laue, K;Drzezga, A;Schildhaus, H;Wolf, J;Herrmann, K;Schuler, M;1171P A phase II theranostic study with osimertinib in patients with EGFR-mutated non-small cell lung cancer (NSCLC) progressing on EGFR tyrosine kinase inhibitors (TKI) and undetectable EGFR T790M (THEROS)Annals of Oncologyovartis Oncology; Financial Interests, Personal, Full or part-time Employment: Targos Molecular Pathology, Inc. J. Wolf: Financial Interests, Personal, Advisory Board: Amgen, AstraZeneca, Bayer, Blueprint, BMS, Boehringer Ingelheim, Chugai, Daiichi SFull Paper
2022Chansaenroj, A;Adine, C;Charoenlappanit, S;Roytrakul, S;Sariya, L;Osathanon, T;Rungarunlert, S;Urkasemsin, G;Chaisuparat, R;Yodmuang, S;Souza, GR;Ferreira, JN;Magnetic bioassembly platforms towards the generation of extracellular vesicles from human salivary gland functional organoids for epithelial repairBioactive materials35387159d EV; and (3) to identify novel signaling cues in EV that may optimize organoid development towards epithelial SG repair. 2 Materials and methods 2.1 Adult stem cell culture hDPSC were obtained from AllCells (Alameda, CA, USA) and cultured in DulbecFull Paper
2022Kavanagh, ME;Horning, BD;Khattri, R;Roy, N;Lu, JP;Whitby, LR;Ye, E;Brannon, JC;Parker, A;Chick, JM;Eissler, CL;Wong, AJ;Rodriguez, JL;Rodiles, S;Masuda, K;Teijaro, JR;Simon, GM;Patricelli, MP;Cravatt, BF;Selective inhibitors of JAK1 targeting an isoform-restricted allosteric cysteineNature chemical biology36097295In vitro TE50 values for JAK1_C817 and TYK2_C838 were obtained by treating 500 µg cell lysate generated from primary human PBMCs (AllCells), Jurkat T cells (ATCC, #TIB-152), or MDA-MB-468 breast cancer cells (ATCC, #HTB-132), with DMSO or compound foFull Paper
2022Lam, J;Lee, B;Yu, J;Kwee, BJ;Kim, Y;Kim, J;Choi, Y;Yoon, JS;Kim, Y;Baek, K;Jeon, NL;Sung, KE;A microphysiological system-based potency bioassay for the functional quality assessment of mesenchymal stromal cells targeting vasculogenesisBiomaterials36201944Human bone marrow-derived MSCs were obtained from eight different donors purchased from RoosterBio (RB9, RB12, RB14, RB16; Frederick, MD) at passage 1 (P1), Lonza (8F560, 167,696; Walkersville, MD) at passage 2 (P2), and AllCells (PCBM1632, PCBM1641;Full Paper
2022Han, C;Khodadadi-Jamayran, A;Lorch, AH;Jin, Q;Serafin, V;Zhu, P;Politanska, Y;Sun, L;Gutierrez-Diaz, BT;Pryzhkova, MV;Abdala-Valencia, H;Bartom, ET;Buldini, B;Basso, G;Velu, SE;Sarma, K;Mattamana, BB;Cho, BK;Obeng, RC;Goo, YA;Jordan, PW;Tsirigos, A;Zhou, Y;Ntziachristos, P;SF3B1 homeostasis is critical for survival and therapeutic response in T cell leukemiaScience advances35061527ing PCR to detect the TCRb-NOTCH1 translocation (TCRBJ2S4CUTLL1F: 5′-GGACCCGGCTCTCAGTGCT-3′, NOTCH1CUTTL1R: 5′-TCCCGCCCTCCAAAATAAGG-3′). Human CD3+, CD8+, and CD4+ T cells were purchased from AllCells.com (Alameda, CA) or from Astarte Biologics (BothFull Paper
2022Pan, Z;Chen, J;Xiao, X;Xie, Y;Jiang, H;Zhang, B;Lu, H;Yuan, Y;Han, L;Zhou, Y;Zong, H;Wang, L;Sun, R;Zhu, J;Characterization of a novel bispecific antibody targeting tissue factor-positive tumors with T cell engagementActa pharmaceutica Sinica. B35847491for the T cell activation test were bought from AllCells (Shanghai, China). PBMC used in other experiments were isolated from leukocyte concentrate (AllCells) using Ficoll-Paque PLUS ( Save;nded conditions and used within 2 months after resuscitatioFull Paper
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2022Peng, S;Hu, P;Xiao, YT;Lu, W;Guo, D;Hu, S;Xie, J;Wang, M;Yu, W;Yang, J;Chen, H;Zhang, X;Zhu, Y;Wang, Y;Yang, Y;Zhu, G;Chen, S;Wang, J;Zhang, B;Chen, W;Wu, H;Sun, Z;Ding, T;Zhang, H;Yi, Z;Liu, M;Ren, S;Single-Cell Analysis Reveals EP4 as a Target for Restoring T-Cell Infiltration and Sensitizing Prostate Cancer to ImmunotherapyClinical cancer research : an official journal of the American Association for Cancer Research34740924Purpose: Immunotherapies targeting immune checkpoint molecules have shown promising treatment for a subset of cancers; however, many "cold" tumors, such as prostate cancer, SaveFull Paper
2022Siegel, RJ;Singh, AK;Panipinto, PM;Shaikh, FS;Vinh, J;Han, SU;Kenney, HM;Schwarz, EM;Crowson, CS;Khuder, SA;Khuder, BS;Fox, DA;Ahmed, S;Extracellular sulfatase-2 is overexpressed in rheumatoid arthritis and mediates the TNF-?-induced inflammatory activation of synovial fibroblastsCellular & molecular immunology36068294Human serum samples were purchased from commercial vendors (Discovery Life Sciences, Newtown, PA; Innovative Research, Novi, MI; or Sanguine Biosciences, Los Angeles, CA) or generously shared by Dr. Cynthia Crowson (Mayo Clinic, Rochester, MN)Full Paper
2022Gyurdieva, A;Zajic, S;Chang, YF;Houseman, EA;Zhong, S;Kim, J;Nathenson, M;Faitg, T;Woessner, M;Turner, DC;Hasan, AN;Glod, J;Kaplan, RN;D'Angelo, SP;Araujo, DM;Chow, WA;Druta, M;Demetri, GD;Van Tine, BA;Grupp, SA;Fine, GD;Eleftheriadou, I;Biomarker correlates with response to NY-ESO-1 TCR T cells in patients with synovial sarcomaNature communications36075914s were digitally obtained using CaseViewer (3D Histech, Budapest, Hungary). Protein expression by IHC NY-ESO-1 staining was performed using the E978 clone (Sigma, Catalog #: N2038, at 1??g/mL) at QualTek Laboratory (Goleta, CA; now part of DiscoveryFull Paper
2022Ni, Z;Sun, P;Zheng, J;Wu, M;Yang, C;Cheng, M;Yin, M;Cui, C;Wang, G;Yuan, L;Gao, Q;Li, Y;JNK Signaling Promotes Bladder Cancer Immune Escape by Regulating METTL3-Mediated m6A Modification of PD-L1 mRNACancer research35502544CD8+ T cells (catalog no. PB009-3F-C) were obtained from AllCells (Shanghai) and cultured in ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies, catalog no. 10981). Seventy-two hours after activation with ImmunoCult Human CD3/CD28/CD2 T CelFull Paper
2022Ge, X;Yost, SE;Lee, JS;Frankel, PH;Ruel, C;Cui, Y;Murga, M;Tang, A;Martinez, N;Chung, S;Yeon, C;Stewart, D;Li, D;Rajurkar, S;Somlo, G;Mortimer, J;Waisman, J;Yuan, Y;Phase II Study Combining Pembrolizumab with Aromatase Inhibitor in Patients with Metastatic Hormone Receptor Positive Breast CancerCancers36077811mal TILs were measured by hematoxylin and eosin (H&E) staining of formalin-fixed paraffin-embedded (FFPE) tumor biopsies according to the International TIL Work Group guideline [16]. PD-L1 analysis: QualTek Molecular Laboratory (Goleta, CA, USA) perFull Paper
2022Shirk, B;Shirk, P;Furlong, R;Scully, E;Wu, K;Siegfried, B;Gene Editing of the ABC Transporter/White Locus Using Crispr/Cas9-Mediated Mutagenesis in the Indian Meal MothSSRN Electronic Journaly and sequencing, and Kris Hartzer for laboratory assistance. Library 497 preparation and sequencing were performed at HudsonAlpha Genomic Services Lab 498 (Huntsville, AL). We also thank Karen Garren for her work to isolate the P. interpunctella 499Full Paper
2022Kanter, J;Thompson, AA;Pierciey, FJ;Hsieh, M;Uchida, N;Leboulch, P;Schmidt, M;Bonner, M;Guo, R;Miller, A;Ribeil, JA;Davidson, D;Asmal, M;Walters, MC;Tisdale, JF;Lovo-cel gene therapy for sickle cell disease: Treatment process evolution and outcomes in the initial groups of the HGB-206 studyAmerican journal of hematology36161320MCW served as medical director for AllCells and as a consultant for AllCells, Ensoma, Vertex, and BioLabs. The remaining authors declare no competing financial interests. SaveFull Paper
2022Wei, Y;Kanagal-Shamanna, R;Zheng, H;Bao, N;Lockyer, PP;Class, CA;Darbaniyan, F;Lu, Y;Lin, K;Yang, H;Montalban-Bravo, G;Ganan-Gomez, I;Soltysiak, KA;Do, KA;Colla, S;Garcia-Manero, G;Cooperation between KDM6B overexpression and TET2 deficiency in the pathogenesis of chronic myelomonocytic leukemiaLeukemia35697791Materials and methods Human samples BM specimens were obtained from the MD Anderson Cancer Center tissue bank following institutional guidelines. BM samples from healthy individuals were obtained from AllCells (Emeryville, CA)Full Paper
2022Lu, M;Xia, L;Elmansy, N;Clementelli, C;Tremblay, D;Hoffman, R;Combined Drug Targeting of p53-dependent and -independent Pathways Depletes Myelofibrosis Hematopoietic Stem/Progenitor CellsLeukemia34642468met the World Health Organization criteria for the diagnosis of MF [41, 42]. Patient characteristics are provided in Supplemental Table 1. Normal donor (ND) human bone marrow (BM) was purchased from AllCells (Emeryville, CA). The experiments in whichFull Paper
2022Bae, J;Accardi, F;Hideshima, T;Tai, YT;Prabhala, R;Shambley, A;Wen, K;Rowell, S;Richardson, PG;Munshi, NC;Anderson, KC;Targeting LAG3/GAL-3 to overcome immunosuppression and enhance anti-tumor immune responses in multiple myelomaLeukemia34290359roval by the Institutional Review Board at Dana-Farber Cancer Institute (Boston, MA). In addition, healthy individuals BM [N = 5] or leukapheresis [N = 12] products were purchased from either AllCells (Alameda, CA) or the Blood Donor Center at BostonFull Paper
2022Huang, G;Cheng, Z;Hildebrand, A;Wang, C;Cimini, M;Roy, R;Lucchese, AM;Benedict, C;Mallaredy, V;Magadum, A;Joladarashi, D;Thej, C;Gonzalez, C;Trungcao, M;Garikipati, VNS;Elrod, JW;Koch, WJ;Kishore, R;Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibitionTheranostics35673580days, db/+ EPCs were cultured at 37°C, 5% CO2 atmosphere. Male mice were used to avoid the reproductive hormone effect in female mice. Human CD34+ Hematopoietic stem cells (HSC) were purchased from AllCells, LLC and were cultured in a human hematopoiFull Paper
2022Shen, Y;Eng, JS;Fajardo, F;Liang, L;Li, C;Collins, P;Tedesco, D;Nolan-Stevaux, O;Cancer cell-intrinsic resistance to BiTE therapy is mediated by loss of CD58 costimulation and modulation of the extrinsic apoptotic pathwayJournal for immunotherapy of cancer35296559Target cells were cocultured with freshly thawed human CD3 + T cells (ALLCELLS) and increasing concentrations of TCEs. Assay conditions were effector-to-target ratio (E:T)=2:1 and SaveFull Paper
2022Froning, KJ;Sereno, A;Huang, F;Demarest, SJ;Generalizable design parameters for soluble T cell receptor-based T cell engagersJournal for immunotherapy of cancer35260435ously11 with minor modifications throughout the protocol. Saos-2 and HCT-116 cells were cultured as described earlier. Primary naïve T cells were from StemExpress (cat#PB03020C, Donor#D001003581) or AllCells (cat# PB009-1F, Lt# 3009286). For the assaFull Paper
2022Wu, ZH;Li, N;Mei, XF;Chen, J;Wang, XZ;Guo, TT;Chen, G;Nie, L;Chen, Y;Jiang, MZ;Wang, JT;Wang, HB;Preclinical characterization of the novel anti-SIRPα antibody BR105 that targets the myeloid immune checkpointJournal for immunotherapy of cancer35256517Allogeneic PBMCs (AllCells) from different donors were added at a dendritic cell:PBMCs ratio of 1:20. Indicated mAbs were added at a concentration of 10 µg/mL immediately, and the SaveFull Paper
2022Lee, KM;Lin, CC;Servetto, A;Bae, J;Kandagatla, V;Ye, D;Kim, G;Sudhan, DR;Mendiratta, S;González Ericsson, PI;Balko, JM;Lee, J;Barnes, S;Malladi, VS;Tabrizi, S;Reddy, SM;Yum, S;Chang, CW;Hutchinson, KE;Yost, SE;Yuan, Y;Chen, ZJ;Fu, YX;Hanker, AB;Arteaga, CL;Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast CancerCancer immunology research35561311After a week, 1 A 107 human PBMCs (hPBMC; ALLCELLS) were injected intravenously. Two weeks later, SUM159PT tumors were harvested for analysis of tumor-infiltrating immune SaveFull Paper
2022Li, W;Yang, S;Xu, P;Zhang, D;Tong, Y;Chen, L;Jia, B;Li, A;Lian, C;Ru, D;Zhang, B;Liu, M;Chen, C;Fu, W;Yuan, S;Gu, C;Wang, L;Li, W;Liang, Y;Yang, Z;Ren, X;Wang, S;Zhang, X;Song, Y;Xie, Y;Lu, H;Xu, J;Wang, H;Yu, W;SARS-CoV-2 RNA elements share human sequence identity and upregulate hyaluronan via NamiRNA-enhancer networkEBioMedicine35124429Human umbilical vein endothelial cells (HUVEC) were cultured in the commercial culture medium (AllCells, Cat# H-004). VERO C1008 (E6) (Cat# GNO17) and its culture medium MEM Save;ed with 10% FBS (Gibco, Cat# 10270-106) and 1% Penicillin-StreptomyciFull Paper
2022Zhang, C;Chen, Z;Liu, J;Wu, M;Yang, J;Zhu, Y;Lu, W;Ruan, C;3D-printed pre-tapped-hole scaffolds facilitate one-step surgery of predictable alveolar bone augmentation and simultaneous dental implantationComposites Part B: EngineeringThe human bone marrow mesenchymal stem cells (hBMSCs, obtained from Allcells, Silicon Valley, CA) were cultured in α-MEM (Gibco, USA) supplemented with 1% penicillin-streptomycin (Gibco, USA) and 10% fetal bovine serum (FBS) (Gibco, USA) in an atmospFull Paper
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2022Suyama, T;Takemoto, Y;Miyauchi, H;Kato, Y;Matsuzaki, Y;Kato, R;Morphology-based noninvasive early prediction of serial-passage potency enhances the selection of clone-derived high-potency cell bank from mesenchymal stem cellsInflammation and regeneration36182958Bone marrow mononuclear cells were prepared from bone marrow aspirate (AllCells, Alameda, USA) collected from healthy donors using density gradient centrifugation with Ficoll (GE Save CachedFull Paper
2022Wang, J;Jiang, J;Yang, X;Zhou, G;Wang, L;Xiao, B;Tethering Piezo channels to the actin cytoskeleton for mechanogating via the cadherin-β-catenin mechanotransduction complexCell reports35139384Experimental models: Cell lines HEK293T Xiao et al., 2011 N/A MDCK Xu Tan Lab in Tsinghua University N/A HUVEC Purchased from Allcells N/AFull Paper
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2022Zhang, J;Liu, F;Yang, Y;Yu, N;Weng, X;Yang, Y;Gong, Z;Huang, S;Gan, L;Sun, S;Zhang, X;Gong, Y;Liu, Y;Guo, W;Integrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancerCell death & disease35449126ze the results using the positive control contained in the different membranes. T-cell amplification and tumor-reactive T cell killing assay Peripheral blood mononuclear cells (PBMCs) purchased from AllCells (China) were activated with anti-CD3 antiFull Paper
2022Liu, P;Zhang, Q;Mi, J;Wang, S;Xu, Q;Zhuang, D;Chen, W;Liu, C;Zhang, L;Guo, J;Wu, X;Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246Stem cell research & therapy35241153Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Cat. H-001F, AllCells) and were cultured in DMEM (Cat. SH30021.01, Hyclone) with 4.5 g/L D-Glucose SaveFull Paper
2022McLemore, AF;Hou, HA;Meyer, BS;Lam, NB;Ward, GA;Aldrich, AL;Rodrigues, MA;Vedder, A;Zhang, L;Padron, E;Vincelette, ND;Sallman, DA;Abdel-Wahab, O;List, AF;McGraw, KL;Somatic gene mutations expose cytoplasmic DNA to co-opt the cGAS/STING/NLRP3 axis in myelodysplastic syndromesJCI insight35788117using Ficoll-Paque (GE Healthcare) from patients with MDS and healthy donors who provided informed consent at the Moffitt Cancer Center or NTUH or were purchased from AllCells. Save CachedFull Paper
2022Xie, J;Gui, X;Deng, M;Chen, H;Chen, Y;Liu, X;Ku, Z;Tan, L;Huang, R;He, Y;Zhang, B;Lewis, C;Chen, K;Xu, L;Xu, J;Huang, T;Liao, XC;Zhang, N;An, Z;Zhang, CC;Blocking LAIR1 signaling in immune cells inhibits tumor developmentFrontiers in immunology36211388Human allogeneic pan T cells (PB009-1F, Allcells) were stained with Carboxy fluorescein succinimidyl ester (CFSE) (C34554, Thermo Fisher Scientific), and mixed with 3 x10 5 mature SaveFull Paper
2022Hu, JF;Wang, ZW;Liao, CY;Chen, ZW;Kang, FP;Lin, CF;Lin, TS;Huang, L;Tian, YF;Chen, S;Induced expression of CCL19 promotes the anti-tumor ability of CAR-T cells by increasing their infiltration abilityFrontiers in immunology35990619(CRL-1682, ATCC) cells were obtained from ATCC and cultured in RPMI-1640 medium (cat. no. 11875093; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. Human T cells were obtained from Allcells (cat. no. PB009-CD3-F). Both of the cellsFull Paper
2022Bjornson-Hooper, ZB;Fragiadakis, GK;Spitzer, MH;Chen, H;Madhireddy, D;Hu, K;Lundsten, K;McIlwain, DR;Nolan, GP;A Comprehensive Atlas of Immunological Differences Between Humans, Mice, and Non-Human PrimatesFrontiers in immunology35359965apeutics between humans and model organisms will greatly improve the evaluation of therapeutic candidates. Materials and Methods Blood Venous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human subFull Paper
2022Peng, H;Nerreter, T;Mestermann, K;Wachter, J;Chang, J;Hudecek, M;Rader, C;ROR1-targeting switchable CAR-T cells for cancer therapyOncogene35859167Pan-T cells were isolated from fresh PBMC of healthy donors (Allcells) by negative selection (Pan T Cell Isolation Kit; Miltenyi Biotec), activated using Dynabeads Human T-Activator SaveFull Paper
2022Zhang, M;Zhu, Y;Zhu, J;Xie, Y;Wu, R;Zhong, J;Qiu, Z;Jiang, L;circ_0086296 induced atherosclerotic lesions via the IFIT1/STAT1 feedback loop by sponging miR-576-3pCellular & molecular biology letters36138395HUVECs were obtained from AllCells (Shanghai, China) as previously reported [7, 33]. HUVECs were seeded in EC complete growth medium at 37 C. Then, 100 μg/mL ox-LDL was Save CachedFull Paper
2022Abdul-Aziz, A;Weigel, C;Kovacs, A;Wu, Y;Byrd, J;Hertlein, E;Oakes, C;P381: DNA METHYLATION PROFILING OF MESENCHYMAL STROMAL CELLS ISOLATED FROM FEMURAL HEAD BONE MARROW VERSUS BONE MARROW ASPIRATES: RELEVANCE FOR AML STUDY BASED CONTROLSHemaSphereAspirate-derived BMSCs (n= 7) were expanded from bone marrow mononuclear cells obtained from a commercial source (AllCells). For genome-wide DNA methylation analyses, the SaveFull Paper
2022Owens, TD;Brameld, KA;Verner, EJ;Ton, T;Li, X;Zhu, J;Masjedizadeh, MR;Bradshaw, JM;Hill, RJ;Tam, D;Bisconte, A;Kim, EO;Francesco, M;Xing, Y;Shu, J;Karr, D;LaStant, J;Finkle, D;Loewenstein, N;Haberstock-Debic, H;Taylor, MJ;Nunn, P;Langrish, CL;Goldstein, DM;Discovery of Reversible Covalent Bruton's Tyrosine Kinase Inhibitors PRN473 and PRN1008 (Rilzabrutinib)Journal of medicinal chemistry35302767The ability of selected compounds to block BCR-driven activation of B cells was assessed by expression of the CD69 surface marker, as previously described. (37) Briefly, HWB (AllCells, Alameda, CA, USA) pretreated for 1 h with serial dilutions of tesFull Paper
2022Zheng, L;Wang, H;Zuo, P;Liu, Y;Xu, H;Ye, BC;Rapid On-Chip Isolation of Cancer-Associated Exosomes and Combined Analysis of Exosomes and Exosomal ProteinsAnalytical chemistry35575685The biotin-labeled rabbit antihuman CD63 antibody (anti-CD63) was purchased from AllCells Biotech Co., Ltd. (Shanghai, China). The horseradish peroxidase (HRP)-conjugated secondary antibody, rabbit anti-human CD9 polyclonal antibody, and bicinchoniniFull Paper
2022Avanzino, BC;Prabhakar, K;Dalvi, P;Hartstein, S;Kehm, H;Balasubramani, A;Boudreau, AA;Buelow, B;Chang, K;Davison, LM;Iyer, S;Kalwit, V;Lewis Wilson, K;Malik-Chaudhry, HK;Pierson, W;Pineda, G;Rangaswamy, US;Saiganesh, S;Schellenberger, U;Ugamraj, HS;Yabut, RD;Buelow, R;Chapman, J;Trinklein, ND;Harris, KE;A T-cell engaging bispecific antibody with a tumor-selective bivalent folate receptor alpha binding arm for the treatment of ovarian cancerOncoimmunology36016696In a similar follow-on study, NCG mice were injected with activated human PBMCs (AllCells). Prior to injection, PBMCs were activated for 3 d with 1 µg/mL plate-coated anti-CD3 ( SaveFull Paper
2022Xue, W;Zhang, Q;Chen, Y;Zhu, Y;Hydrogen Sulfide Improves Angiogenesis by Regulating the Transcription of pri-miR-126 in Diabetic Endothelial CellsCells36078059The HUVECs from passages 4 to 7 were used in the experiment and were cultured in endothelial completed medium (ALLCELLS, Shanghai, China) at 37 C in 5% CO 2 . The HUVECs Save CachedFull Paper
2022Sharma, M;Bellio, MA;Benny, M;Kulandavelu, S;Chen, P;Janjindamai, C;Han, C;Chang, L;Sterling, S;Williams, K;Damianos, A;Batlahally, S;Kelly, K;Aguilar-Caballero, D;Zambrano, R;Chen, S;Huang, J;Wu, S;Hare, JM;Schmidt, A;Khan, A;Young, K;Mesenchymal Stem Cell-derived Extracellular Vesicles Prevent Experimental Bronchopulmonary Dysplasia Complicated By Pulmonary HypertensionStem cells translational medicine35758326es, and isotype control was performed on P2 WJ-MSC to confirm the MSC identity. BM-MSC Production BM-MSCs were isolated from fresh adult bone marrow aspirates, and obtained from a commercial source (AllCells, Alameda, CA). Briefly, bone marrow was pFull Paper
2022Ohori-Morita, Y;Niibe, K;Limraksasin, P;Nattasit, P;Miao, X;Yamada, M;Mabuchi, Y;Matsuzaki, Y;Egusa, H;Novel Mesenchymal Stem Cell Spheroids with Enhanced Stem Cell Characteristics and Bone Regeneration AbilityStem cells translational medicine35267026heir therapeutic potential in bone regeneration. Materials and Methods BM-MSC Adherent Culture Human BM-MSCs of an 18-year-old male (donor 1; Lonza, Basel, Switzerland), a 22-year-old male (donor 2; AllCells, Alameda, CA, USA), and a 24-year-old malFull Paper
2022Stratmann, S;Yones, SA;Garbulowski, M;Sun, J;Skaftason, A;Mayrhofer, M;Norgren, N;Herlin, MK;Sundström, C;Eriksson, A;Höglund, M;Palle, J;Abrahamsson, J;Jahnukainen, K;Munthe-Kaas, MC;Zeller, B;Tamm, KP;Cavelier, L;Komorowski, J;Holmfeldt, L;Transcriptomic analysis reveals proinflammatory signatures associated with acute myeloid leukemia progressionBlood advances34619772children (Table 1). Detailed biological characteristics and supporting clinical information are reported in supplemental Tables 2 and 3. CD34-expressing bone marrow (BM) cells from 5 healthy donors (AllCells Inc., Alameda, CA) were used as normal conFull Paper
2022Jeon, E;Choi, D;Choi, S;Won, J;Jo, Y;Kim, H;Jung, Y;Shin, S;Min, H;Choi, H;Lee, M;Park, Y;Chung, J;Jin, H;Enhancing adoptive T‐cell therapy with fucoidan‐based IL ‐2 delivery microcapsulesBioengineering & Translational MedicineCryopreserved human peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryvill, CA). Total CD3+T cells were isolated by negative selection with a magnetic isolation kit and CD4+T, CD8+T, and regulatory T (Treg) cells were thenFull Paper
2022Lun, Y;Zhou, Y;Li, Q;Chen, P;Huang, Y;Ye, G;Preparation and characterization of a magnetic microsphere synthesized from sucrose allyl ether for transcatheter arterial embolizationMaterials Today ChemistryHuman umbilical vein endothelial cells (HUVECs, AllCells, US) were subcultured in a low glucose Dulbecco's modified eagle medium containing 10% fetal bovine serum and 1% penicillin/streptomycin (100 U·mL−1) in an atmosphere of 310.15 K and 5% CO2Full Paper
2022Lu, W;Zhou, C;Ma, Y;Li, J;Jiang, J;Chen, Y;Dong, L;He, F;Improved osseointegration of strontium-modified titanium implants by regulating angiogenesis and macrophage polarizationBiomaterials science35384947HUVECs together with complete and basal medium were purchased from Allcells Bio (Shanghai, China). The murine macrophage cell lines (Raw264.7) were obtained from the SaveFull Paper
2022Zhou, Y;Li, XH;Xue, WL;Jin, S;Li, MY;Zhang, CC;Yu, B;Zhu, L;Liang, K;Chen, Y;Tao, BB;Zhu, YZ;Wang, MJ;Zhu, YC;YB-1 Recruits Drosha to Promote Splicing of pri-miR-192 to Mediate the Proangiogenic Effects of H2SAntioxidants & redox signaling35044231HUVECs were purchased from Allcells (Shanghai, China) and cultured in endothelial basal medium (EBM; Allcells, Shanghai, China) supplemented with hydrocortisone, bovine brain SaveFull Paper
2022Li, J;Hong, T;Wei, Y;Guo, L;Lee, M;Yang, H;Class, C;Yang, Y;Wang, X;He, H;Siwko, S;You, MJ;Zhou, Y;Garcia-Manero, G;Huang, Y;Aberrant DNA hydroxymethylation reshapes transcription factor binding in myeloid neoplasmsClinical epigenetics35765052BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). BM mononuclear cells were isolated using a Ficoll-Paque density gradient media (GE, Sweden). Save CachedFull Paper
2022Fang, J;Li, R;Zhang, Y;Oduro, PK;Li, S;Leng, L;Wang, Z;Rao, Y;Niu, L;Wu, HH;Wang, Q;Aristolone in Nardostachys jatamansi DC. induces mesenteric vasodilation and ameliorates hypertension via activation of the KATP channel and PDK1-Akt-eNOS pathwayPhytomedicine : international journal of phytotherapy and phytopharmacology35738117Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells Biotech Co. Ltd. (Shanghai, China) and cultured in Endothelial Cell Medium (ECM; AllCells Biotech Co. Ltd., Shanghai, China) with 10% fetal bovine serum, 1% endothelial growtFull Paper
2022Liang, W;Chen, J;Zheng, H;Lin, A;Li, J;Wu, W;Jie, Q;MiR-199a-5p-containing macrophage-derived extracellular vesicles inhibit SMARCA4 and alleviate atherosclerosis by reducing endothelial cell pyroptosisCell biology and toxicology35930100Human monocyte-derived macrophages (hMDMs, PB-MDM-001F, AllCells) were purchased from Milestone Biotechnologies (Shanghai, China). The hMDMs were cultured at 37 °C in RPMI1640 medium (supplemented with 10% FBS and 1% penicillin/streptomycin) with 5%Full Paper
2022Wang, J;Li, C;He, K;Kuang, Z;Lu, J;Yao, Y;He, F;Li, N;Li, L;Fu, F;Wu, Z;Zhou, S;Kang, D;Qiu, X;Wu, M;Liu, Y;Cao, X;Xu, M;Chen, B;Wu, W;Guo, F;Characterization of anti-CD79b/CD3 bispecific antibody, a potential therapy for B cell malignanciesCancer immunology, immunotherapy : CII35963895Materials and methods Reagents IBI38D9 and human IgG1 were produced by Innovent Biologics Co., Ltd. (Suzhou, China). Human IgG was purchased from Equitech-Bio. Peripheral blood mononuclear cells (PBMCs) were purchased from the AllCellsFull Paper
2022Ni, H;Cao, L;Wu, Z;Wang, L;Zhou, S;Guo, X;Gao, Y;Jing, H;Wu, M;Liu, Y;Ding, J;Zhang, P;Zhou, Y;Chen, B;Xiong, Y;Sun, J;Prinz, B;Baruah, H;Geoghegan, J;Yu, M;Wu, W;Liu, J;Combined strategies for effective cancer immunotherapy with a novel anti-CD47 monoclonal antibodyCancer immunology, immunotherapy : CII34165607CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMCs, Allcells) and then differentiated into macrophages following incubation for 7 days with granulocyte- SaveFull Paper
2022Wu, ZH;Li, N;Gao, ZZ;Chen, G;Nie, L;Zhou, YQ;Jiang, MZ;Chen, Y;Chen, J;Mei, XF;Hu, F;Wang, HB;Development of the Novel Bifunctional Fusion Protein BR102 That Simultaneously Targets PD-L1 and TGF-β for Anticancer ImmunotherapyCancers36230887IgG1 isotype control was expressed by Biointron Biological (Taizhou, China). 2.2. Cell Lines and Cell Culture Dendritic cells (DCs) and peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Shanghai, China). TF-1 cells were from tFull Paper
2022Wang, X;Chen, G;Nie, L;Wu, Z;Wang, X;Pan, C;Chen, X;Zhao, X;Zhu, J;He, Q;Wang, H;IL-2K35C-moFA, a Long-Acting Engineered Cytokine with Decreased Interleukin 2 Receptor α Binding, Improved the Cellular Selectivity Profile and Antitumor Efficacy in a Mouse Tumor ModelCancers36230665Next, 100 μL CellTiter-Glo (G7572, Promega Corporation, Madison, WI, USA) was added; luminescence was recorded suing a Spectramax M5. 2.8. p-STAT5 Assay in Treg and CD8+ T Cell Tregs (PB009-4F-2, Allcells, Shanghai, China) at a density of 1 × 106 cFull Paper
2022Sekhri, P;Ledezma, DK;Shukla, A;Sweeney, EE;Fernandes, R;The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma CellsCancers35326601Healthy donor peripheral blood leukopaks were purchased from AllCells (Alameda, CA, USA) along with the HLA report. Peripheral blood mononuclear cells (PBMCs) were isolated by SaveFull Paper
2022Ning, K;Roy, A;Cheng, F;Xu, P;Kleiboeker, S;Escalante, CR;Wang, J;Qiu, J;High-Throughput Screening Identifies Inhibitors for Parvovirus B19 Infection of Human Erythroid ProgenitorsJournal of virology34669461Primary human CD34+ hematopoietic stem cells (HSCs), which were isolated from the bone marrow of a healthy human donor, were purchased from AllCells LLC (Alameda, CA). B19V-containing plasma samples were obtained from ViraCor Eurofins Laboratories (LFull Paper
2022Liu, Y;Tsang, K;Mays, M;Hansen, G;Chiecko, J;Crames, M;Wei, Y;Zhou, W;Fredrick, C;Hu, J;Liu, D;Gebhard, D;Huang, ZF;Datar, A;Kronkaitis, A;Gueneva-Boucheva, K;Seeliger, D;Han, F;Sen, S;Kasturirangan, S;Scheer, JM;Nixon, AE;Panavas, T;Marlow, MS;Kumar, S;An adapted consensus protein design strategy for identifying globally optimal biotherapeuticsmAbs35613320isolated from human peripheral blood mononuclear cells (PBMCs) using a negative selection kit (EasySep). PBMCs were isolated from Leukopak collected from healthy volunteers, which were obtained from ALLcells. Redirected T-cell cytotoxicity was assayeFull Paper
2022Zhao, J;Jiang, L;Yang, H;Deng, L;Meng, X;Ding, J;Yang, S;Zhao, L;Xu, W;Wang, X;Zhu, Z;Huang, H;A strategy for the efficient construction of anti-PD1-based bispecific antibodies with desired IgG-like propertiesmAbs35239451#F9512) at 4°C for 1 h. Cells were washed three times and then analyzed on the CytoFLEX Cytometer System (Beckman Coulter). Allogeneic mixed lymphocyte reaction Monocytes were enriched from PBMCs (AllCells, Cat# PB002-C-300) by adherence on tissue cFull Paper
2022McGuire, BE;Mela, JE;Thompson, VC;Cucksey, LR;Stevens, CE;McWhinnie, RL;Winkler, DFH;Pelech, S;Nano, FE;Escherichia coli recombinant expression of SARS-CoV-2 protein fragmentsMicrobial cell factories35123472mall number of serum or plasma samples from positive tested donors and all pre-COVID-19 samples were acquired by purchase (Precision for Medicine, Norton, MA, USA; Innovative Research, Novi, MI, USA; AllCells, Alameda, CA, USA) or by donation (CureImFull Paper
2022Li, G;Reid, KM;Spitler, K;Beatty, N;Boucher, J;Davila, ML;CD3 engagement as a new strategy for allogeneic "off-the-shelf" T cell therapyMolecular therapy oncolytics35317526lls was harvested, and 0.45 μm was filtered and cryopreserved for future use. For T cell transduction, healthy donor leukopaks were purchased from Stemcell Technologies (Vancouver, BC, Canada) and ALLCELLS (Alameda, CA). PBMCs were isolated using staFull Paper
2022Biscaia, S;Branquinho, MV;Alvites, RD;Fonseca, R;Sousa, AC;Pedrosa, SS;Caseiro, AR;Guedes, F;Patrício, T;Viana, T;Mateus, A;Maurício, AC;Alves, N;3D Printed Poly(????-caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on a Composite Preparation by Melt Blending or Solvent Casting Techniques and the Influence of Bioceramic Content on Scaffold PropertiesInternational journal of molecular sciences35216432ON 4505 equipment in a dry state at a rate of 1 mm/min. Five specimens were tested for each composition. 4.3. Cytocompatibility Analysis 4.3.1. Cell Culture and Maintenance hDPSCs were acquired from AllCells, LLC (Cat. DP0037F, Lot N° DPSC090411-01)Full Paper
2022Cunningham, AW;Jones, M;Frank, N;Sethi, D;Miller, MM;Stem-like memory T cells are generated during hollow fiber perfusion-based expansion and enriched after cryopreservation in an automated modular cell therapy manufacturing processCytotherapy36031522Leukopaks from three healthy donors were acquired by processing whole blood on the Spectra Optia Apheresis System and obtained through AllCells (Alameda, CA, USA) (Fig. 1A). The apheresis product was shipped to the lab overnight, and CD3+ T cells werFull Paper
2022Jimenez, AC;Heist, CA;Navaei, M;Yeago, C;Roy, K;Longitudinal two-dimensional gas chromatography mass spectrometry as a non-destructive at-line monitoring tool during cell manufacturing identifies volatile features correlative to cell product qualityCytotherapy35882596CellBIND 96-well plate (Corning) for 48-h before co-culture at 50 000 cells per well. PBMCs (AllCells, Alameda, CA, USA) were thawed with a CTL anti-aggregate solution (Cellular Technology Ltd, Shaker Heights, OH, USA) and plated in a six-well plateFull Paper
2022Maughon, TS;Shen, X;Huang, D;Michael, AOA;Shockey, WA;Andrews, SH;McRae, JM;Platt, MO;Fernández, FM;Edison, AS;Stice, SL;Marklein, RA;Metabolomics and cytokine profiling of mesenchymal stromal cells identify markers predictive of T-cell suppressionCytotherapy34696960ethylenediaminetetraacetic acid; Gibco) and then seeded at three densities (10 000, 5000 and 2000 cells/well) in a 96-well plate and cultured for 24 h. PBMCs (AllCells, Alameda, CA, USA) were thawed in Roswell Park Memorial Institute medium, 20% fetaFull Paper
2022Xu, C;Yang, J;Kosters, A;Babcock, BR;Qiu, P;Ghosn, EEB;Comprehensive multi-omics single-cell data integration reveals greater heterogeneity in the human immune systemiScience36185375ical samples ------------------------- Human Adult Peripheral Blood Emory University’s Children’s Clinical and Translational Discovery Core N/A Human Adult Bone Marrow Emory University; AllCells N/A ------------------------- Critical coFull Paper
2022Magis, W;DeWitt, MA;Wyman, SK;Vu, JT;Heo, SJ;Shao, SJ;Hennig, F;Romero, ZG;Campo-Fernandez, B;Said, S;McNeill, MS;Rettig, GR;Sun, Y;Wang, Y;Behlke, MA;Kohn, DB;Boffelli, D;Walters, MC;Corn, JE;Martin, DIK;High-level correction of the sickle mutation is amplified in vivo during erythroid differentiationiScience35633935Cryopreserved CD34+ cells were thawed according to AllCells instructions and cultured for 2 days in StemSpan SFEM with CC110 supplement (StemCell Technologies). For each Save;. Methods CD34+cells CD34+ HSPCs homozygous for the sickle mutation wereFull Paper
2022Liu, P;Qin, L;Liu, C;Mi, J;Zhang, Q;Wang, S;Zhuang, D;Xu, Q;Chen, W;Guo, J;Wu, X;Exosomes Derived From Hypoxia-Conditioned Stem Cells of Human Deciduous Exfoliated Teeth Enhance Angiogenesis via the Transfer of let-7f-5p and miR-210-3pFrontiers in cell and developmental biology35557954Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Cat. H-001F, AllCells) and were cultured in DMEM (Cat. SH30021.01, Hyclone) supplemented with 10 SaveFull Paper
2022Li, L;Zhou, B;Xu, H;Shi, H;Gao, L;Ge, B;Zinc-Loaded Black Phosphorus Multifunctional Nanodelivery System Combined With Photothermal Therapy Have the Potential to Treat Prostate Cancer Patients Infected With COVID-19Frontiers in endocrinology35464050n, USA). Human HMGB1 ELISA Kit was obtained from Arigo Biolaboratories (Taiwan, China). ATP content detection kit was obtained from Solarbio (Beijing, China). Dendritic cells (DCs) were obtained from AllCells (San Francisco, USA). FITC anti-human CD1Full Paper
2022McGovern, K;Castro, AC;Cavanaugh, J;Coma, S;Walsh, M;Tchaicha, J;Syed, S;Natarajan, P;Manfredi, M;Zhang, XM;Ecsedy, J;Discovery and Characterization of a Novel Aryl Hydrocarbon Receptor Inhibitor, IK-175, and Its Inhibitory Activity on Tumor Immune SuppressionMolecular cancer therapeutics35666806Naive human T cells (CD4+/CD62L+) from two independent donors were isolated from cryopreserved CD4+ T cells (AllCells) by magnetic selection with human CD62 L microbeads (Miltenyi). On Day 1, naive CD62L+ human T cells were activated with human CD3/CFull Paper
2022Lin, W;Qian, J;Wang, H;Ren, L;Yang, Y;Chen, C;Chen, X;Huang, Y;Liu, J;Xu, N;Teng, L;Cisplatin plus anti-PD-1 antibody enhanced treatment efficacy in advanced esophageal squamous cell carcinomaAmerican journal of cancer research35261780Therapies for patients with advanced esophageal squamous cell carcinoma (ESCC) are limited and accompanied by dismal prognosis. Here we use ESCC cell line K30 and TE-1 to SaveFull Paper
2022Duan, J;Lei, D;Ling, C;Wang, Y;Cao, Z;Zhang, M;Zhang, H;You, Z;Yao, Q;Three-dimensional-printed polycaprolactone scaffolds with interconnected hollow-pipe structures for enhanced bone regenerationRegenerative biomaterials35719204CR MasterMix (ABM, CA) on a Light Cycler 480 II (Roche, CHE). To evaluate the expression of angiogenesis genes (HIF1-α and VEGF), human umbilical vein endothelial cells (HUVECs) were purchased from AllCells. First, 6 × 105 HUVECs were seeded verticaFull Paper
2022Li, Z;Kou, L;Fu, X;Xie, Z;Xu, M;Guo, L;Lin, T;Gong, S;Zhang, S;Liu, M;Design, synthesis, and biological evaluation of triazole-pyrimidine-methylbenzonitrile derivatives as dual A2A/A2B adenosine receptor antagonistsJournal of enzyme inhibition and medicinal chemistry35616298Cryopreserved PBMCs (Allcells) were thawed and recovered in RPMI1640 medium overnight before being used. And compounds to be tested were diluted with DMSO to 100 folds of SaveFull Paper
2022Hu, S;Muniraj, G;Mishra, A;Hong, K;Lum, JL;Hong, CHL;Rosa, V;Sriram, G;Characterization of silver diamine fluoride cytotoxicity using microfluidic tooth-on-a-chip and gingival equivalentsDental materials : official publication of the Academy of Dental Materials35778310Objective This study aims to characterize the cytotoxicity potential of silver diamine fluoride (SDF) on dental pulp stem cells (DPSC) and gingival equivalents. Methods DPSC cultured on SaveFull Paper
2022Dogan, M;Karhan, E;Kozhaya, L;Placek, L;Chen, X;Yigit, M;Unutmaz, D;Engineering Human MAIT Cells with Chimeric Antigen Receptors for Cancer ImmunotherapyJournal of immunology (Baltimore, Md. : 1950)36165183Healthy adult blood was obtained from AllCells (Quincy, MA). PBMCs were isolated using Ficoll-Paque plus (GE Healthcare). CD4 + T, CD8 + T, and CD19 + B cells and CD14 + SaveFull Paper
2022Ben Nasr, M;Robbins, D;Parone, P;Usuelli, V;Tacke, R;Seelam, AJ;Driver, E;Le, T;Sabouri-Ghomi, M;Guerrettaz, L;Shoemaker, D;Fiorina, P;Pharmacologically Enhanced Regulatory Hematopoietic Stem Cells Revert Experimental Autoimmune Diabetes and Mitigate Other Autoimmune DisordersJournal of immunology (Baltimore, Md. : 1950)35321879A total of 5000 cryopreserved and preisolated human mobilized peripheral blood HSCs (CD34 + cells) (AllCells) were plated into each well of a 384-well plate (Corning) in StemSpan- SaveFull Paper
2022Melani, RD;Des Soye, BJ;Kafader, JO;Forte, E;Hollas, M;Blagojevic, V;Negrão, F;McGee, JP;Drown, B;Lloyd-Jones, C;Seckler, HS;Camarillo, JM;Compton, PD;LeDuc, RD;Early, B;Fellers, RT;Cho, BK;Mattamana, BB;Goo, YA;Thomas, PM;Ash, MK;Bhimalli, PP;Al-Harthi, L;Sha, BE;Schneider, JR;Kelleher, NL;Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody RepertoireJournal of proteome research34878788odium heparin (BD 367874, Fisher Scientific) by centrifugation at 1,500 × g for 10 min. Plasma from a convalescent patient featuring a high titer of anti-SARS-CoV-2-RBD antibodies was purchased from AllCells (commercial sample 1 - CS1) and used as aFull Paper
2022Kawato, Y;Fukahori, H;Nakamura, K;Kanno, A;Kubo, K;Hiramitsu, M;Matsuda, T;Hanada, Y;Furukawa, T;Nakajima, Y;Kinugasa, F;Morokata, T;Potential benefit of the cathepsin S inhibitor, ASP1617, as a treatment for systemic lupus erythematosusEuropean journal of pharmacology35157914Five different lots of peripheral blood-derived frozen B cell were purchased from AllCells (Alameda, CA, USA). Cell suspensions (final, 2 × 10 6 SaveFull Paper
2022Pieles, O;Höring, M;Adel, S;Reichert, TE;Liebisch, G;Morsczeck, C;Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle CellsStem cells international35903407Human dental follicle cells were purchased from AllCells (Alameda, CA, USA) and cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS) SaveFull Paper
2022Schlichtner, S;Yasinska, IM;Ruggiero, S;Berger, SM;Aliu, N;Prunk, M;Kos, J;Meyer, NH;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV;Expression of the Immune Checkpoint Protein VISTA Is Differentially Regulated by the TGF-β1 - Smad3 Signaling Pathway in Rapidly Proliferating Human Cells and T LymphocytesFrontiers in medicine35223897Medium was also supplemented with penicillin (50 IU/ml), and streptomycin sulfate (50 μg/ml). Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were also purchased from AllCells (Alameda, CA, USA) and handled according toFull Paper
2022Yoshimaru, K;Yamaza, T;Kajioka, S;Sonoda, S;Yanagi, Y;Matsuura, T;Yoshizumi, J;Oda, Y;Iwata, N;Takai, C;Nakayama, S;Taguchi, T;Dental pulp stem cells as a therapy for congenital entero-neuropathyScientific reports35484137ies were passaged to expand the number of dDPSCs. Cells at P3 were used for the experiments. BMMSCs isolated from human whole bone marrow aspirates obtained from healthy adult volunteers (n = 3; AllCells, Alameda, CA, USA) and human skin fibroblastsFull Paper
2022Herrera, VLM;Walkey, AJ;Nguyen, MQ;Gromisch, CM;Mosaddhegi, JZ;Gromisch, MS;Jundi, B;Lukassen, S;Carstensen, S;Denis, R;Belkina, AC;Baron, RM;Pinilla-Vera, M;Mueller, M;Kimberly, WT;Goldstein, JN;Lehmann, I;Shih, AR;Eils, R;Levy, BD;Ruiz-Opazo, N;A targetable 'rogue' neutrophil-subset, [CD11b+DEspR+] immunotype, is associated with severity and mortality in acute respiratory distress syndrome (ARDS) and COVID-19-ARDSScientific reports35379853ARDS (n = 4) for inertial microfluidic separation (BDL, RMB, MPV) according to respective institutional guidelines. Blood collection Fresh normal peripheral blood neutrophils were procured from AllCells, LLC (CA), which were shipped overnight in IscFull Paper
2022Kim, DE;Shin, YH;Cho, JE;Myung, S;Kim, HG;Kim, KC;Park, CM;Yoon, CH;Identification of 3-Oxindole Derivatives as Small Molecule HIV-1 Inhibitors Targeting Tat-Mediated Viral TranscriptionMolecules (Basel, Switzerland)35956872d fetal bovine serum (all obtained from Gibco-BRL, Gaithersburg, MD, USA). The bl-DTR cells were additionally supplemented with 1 μg/mL puromycin and 200 μg/mL zeocin. The PBMCs were purchased from AllCells (Alameda, CA, USA) and cultured, as describFull Paper
2022Saaid, A;Ensanya, A;Suzina, S;Nurul, A;Samsudin, A;Azlina, A;Odontogenic induction of human amniotic membrane scaffold for dental pulp regenerationMaterials Chemistry and PhysicsFor this study, deciduous teeth stem cells (DTSCs), obtained from ALLCELLS, USA, were used at passage 4-8. The cells were allowed to grow in a-modified Eagle's medium (α-MEM) supplemented with 20% fetal bovine serum (FBS) and 1% penicillin/streptomycFull Paper
2022Jaimes, MC;Leipold, M;Kraker, G;Amir, EA;Maecker, H;Lannigan, J;Full spectrum flow cytometry and mass cytometry: A 32-marker panel comparisonCytometry. Part A : the journal of the International Society for Analytical Cytology35593221Frozen PBMCs from 5 donors were obtained from AllCells Alameda (Alameda, CA). Cells were provided aliquoted in 25 million cells per vial in freezing media and cryopreserved in liquid nitrogen until ready to use. Ethical review and regulatory complianFull Paper
2022Moore, KP;Schwaid, AG;Tudor, M;Park, S;Beshore, DC;Converso, A;Shipe, WD;Anand, R;Lan, P;Moningka, R;Rothman, DM;Sun, W;Chi, A;Cornella-Taracido, I;Adam, GC;Bahnck-Teets, C;Carroll, SS;Fay, JF;Goh, SL;Lusen, J;Quan, S;Rodriguez, S;Xu, M;Andrews, CL;Song, C;Filzen, T;Li, J;Hollenstein, K;Klein, DJ;Lammens, A;Lim, UM;Fang, Z;McHale, C;Li, Y;Lu, M;Diamond, TL;Howell, BJ;Zuck, P;Balibar, CJ;A Phenotypic Screen Identifies Potent DPP9 Inhibitors Capable of Killing HIV-1 Infected CellsACS chemical biology36044633Cells were purchased from AllCells in cryopreserved vials. Cells were centrifuged at 500×g for 10 min at 4 C. The supernatant was removed, and cells were washed with 20 mL PBS. SaveFull Paper
2022Zhang, H;Lv, X;Kong, Q;Tan, Y;IL-6/IFN-γ double knockdown CAR-T cells reduce the release of multiple cytokines from PBMCs in vitroHuman vaccines & immunotherapeutics35049413rward TGGGAGCATGTGAATGCCATCC Reverse GTCGGCTCCTGGAGGTCAAACA β-actin Forward CGCCCCAGGCACCAGGGC Reverse GCTGGGGTGTTGAAGGT 2.3. IL-6/IFN-γ double KD CAR-T cell culture The PBMCs (Allcells, Cat: PB005F) were resuspended in CTSTM AIMFull Paper
2022Zhou, Z;Qin, M;Khodahemmati, S;Li, W;Niu, B;Li, J;Liu, Y;Gao, J;Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysisInternational journal of environmental health research34102927We maintained HUVECs (Allcells, China) routinely in HUVEC complete culture medium (Allcells, China), supplemented with 10% fetal bovine serum (FBS), 2% growth factors, 100 U/mL SaveFull Paper
2022Wang, X;Wong, LM;McElvain, ME;Martire, S;Lee, WH;Li, CZ;Fisher, FA;Maheshwari, RL;Wu, ML;Imun, MC;Murad, R;Toledo Warshaviak, D;Yin, J;Kamb, A;Xu, H;A rational approach to assess off-target reactivity of a dual-signal integrator for T cell therapyToxicology and applied pharmacology35085592Informed-consent for use of primary T cells and donor collection protocols was approved by an Institutional Review Board (IRB) at Allcells or HemaCare. Allcells and HemaCare SaveFull Paper
2022Andrews, SH;Klinker, MW;Bauer, SR;Marklein, RA;Morphological landscapes from high content imaging reveal cytokine priming strategies that enhance mesenchymal stromal cell immunosuppressionBiotechnology and bioengineering34716713Human bone marrow-derived MSCs were obtained from four different donors purchased from Lonza, AllCells, and RoosterBio (see Table S1 for donor information). MSC culture conditions for the Lonza and AllCells (8F3560, 110877, PCBM1662) were chosen baseFull Paper
2022Mandal, S;Sunagawa, SW;Prathipati, PK;Belshan, M;Shibata, A;Destache, CJ;Targeted Immuno-Antiretroviral to Promote Dual Protection against HIV: A Proof-of-Concept StudyNanomaterials (Basel, Switzerland)35683795were maintained in a complete DMEM medium (HiDMEM medium supplemented with 10% FBS and 1× AA) as standard protocol [14,15]. The human peripheral blood mononuclear cells (hPBMCs) were purchased from AllCells (Alameda, CA, USA) and maintained in compFull Paper
2022Huang, P;Zhang, C;Delawary, M;Korchak, JA;Suda, K;Zubair, AC;Development and evaluation of IL-6 overexpressing mesenchymal stem cells (MSCs)Journal of tissue engineering and regenerative medicine34890489Human bone marrow derived mesenchymal stem cells (BMSCs) were isolated from commercial de-identified bone marrow (AllCells, Alameda, CA) from a healthy 28-year-old male SaveFull Paper
2022Harper, T;Sharma, A;Kaliyaperumal, S;Fajardo, F;Hsu, K;Liu, L;Davies, R;Wei, YL;Zhan, J;Estrada, J;Kvesic, M;Nahrwold, L;Deisting, W;Panzer, M;Cooke, K;Lebrec, H;Nolan-Stevaux, O;Characterization of an Anti-CD70 Half-Life Extended Bispecific T-Cell Engager (HLE-BiTE) and Associated On-Target Toxicity in Cynomolgus MonkeysToxicological sciences : an official journal of the Society of Toxicology35583313Human T cells (AllCells) or cynomolgus monkey or human peripheral blood mononuclear cells (PBMCs) (SNBL) were cocultured with target cells at an effector cell (E) to target cell (T) SaveFull Paper
2022Wang, P;Zhang, J;Zhang, H;Zhang, F;The role of MACF1 on acute myeloid leukemia cell proliferation is involved in Runx2-targeted PI3K/Akt signalingMolecular and cellular biochemistry35857251Bone marrow CD34 + hematopoietic stem and progenitor cells (HSPCs) were purchased from AllCells (Alameda, CA, USA) and cultured in X-Vivo 15 media (Lonza, Basel, Switzerland) SaveFull Paper
2022Shams, F;Bayat, H;Mohammadian, O;Mahboudi, S;Vahidnezhad, H;Soosanabadi, M;Rahimpour, A;Advance trends in targeting homology-directed repair for accurate gene editing: An inclusive review of small molecules and modified CRISPR-Cas9 systemsBioImpacts : BI35975201A) Treatment group 1A (unedited HSCs from AllCells). The first mouse was found dead in Treatment group 2B, which contained edited bought HSCs (AllCells), had the highest bone SaveFull Paper
2022Liu, Y;Ma, X;Yang, H;Li, X;Ma, Y;Ason, B;Liu, S;Hu, LA;APLNR Regulates IFN-γ signaling via β-arrestin 1 mediated JAK-STAT1 pathway in melanoma cellsThe Biochemical journal35084016Naive CD8+ T cells used for co-culture assay were obtained from Allcells and cultured in RPMI 1640 medium supplemented with 10% FBS, 1× HEPES, 1× PS, 2 mM Glutamine, 1 mM SaveFull Paper
2022Venn-Watson, SK;Butterworth, CN;Broader and safer clinically-relevant activities of pentadecanoic acid compared to omega-3: Evaluation of an emerging essential fatty acid across twelve primary human cell-based disease systemsPloS one35617322lowed ethical guidelines and documented informed consent: Cell Applications, Inc., CellzDirect, Celsis-IVT, Leukolab, Life Technologies, Lonza, ScienCell, Hemacare Corporation, Stemcell Technologies, AllCells, Physician’s Plasma Alliance, Lifeline CeFull Paper
2022Okada, Y;Kawashima, N;Noda, S;Murano, H;Han, P;Hashimoto, K;Kaneko, T;Okiji, T;VEGFA promotes odonto/osteoblastic differentiation in dental pulp stem cells via ERK/p38 signalingJournal of Dental SciencesSHEDs (P5, DP001F; AllCells, Alameda, CA, USA) were cultured in alpha-modified Eagle's minimum essential medium (α-MEM) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and an antibiotic and antifungal solutiFull Paper
2022Chen, W;Qie, C;Hu, X;Wang, L;Jiang, J;Liu, W;Liu, J;A small molecule inhibitor of VSIG-8 prevents its binding to VISTAInvestigational new drugs35404016PBMCs were purchased from Allcells Biotechnology (Shanghai) Co., LTD, and we got a statement confirming that informed consent was obtained from all subjects, the attachment is on response. Jurkat cells were cultured in RPMI-1640 medium (Biological InFull Paper
2022Han, Y;Bai, X;Wang, X;Exosomal myeloperoxidase as a biomarker of deep venous thrombosisAnnals of translational medicine35242854al microscope (Olympus). HUVECs culture We purchased HUVECs from American Type Culture Collection and cultured them in endothelial cell complete medium containing endothelial cell growth supplement (AllCells, Alameda, CA, USA) at 37 °C in a humidifiFull Paper
2022Sato, M;Fujii, K;Takagi, H;Shibuya, I;Oka, D;Yamaya, N;Hagita, H;Matsumoto, M;Inagaki, K;Antibacterial and Immunosuppressive Effects of OPS-2071, a Candidate Therapy for Inflammatory Bowel DiseaseDigestive diseases and sciences34463880Mouse splenocytes were prepared from eight-week-old female BALB/c mice, and human peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Alameda, CA). To SaveFull Paper
2022Skaddan, MB;Wooten, DW;Wilcox, KC;Voorbach, MJ;Reuter, DR;Jia, ZJ;Foster-Duke, KD;Hickson, JA;Vaidyanathan, S;Reed, AD;Tovcimak, AE;Guo, Q;Comley, RA;Lee, L;Finnema, SJ;Mudd, SR;[18F]BTK-1: A Novel Positron Emission Tomography Tracer for Imaging Bruton's Tyrosine KinaseMolecular imaging and biology35482146Purpose Bruton's tyrosine kinase (BTK) is a key component of B cell receptor (BCR) signaling, and as such a critical regulator of cell proliferation and survival. Aberrant BCR signaling is SaveFull Paper
2022Wu, C;Wang, X;Shang, H;Wei, H;Construction of a Humanized PBMC-PDX Model to Study the Efficacy of a Bacterial Marker in Lung Cancer ImmunotherapyDisease markers36072895, Ltd., and the RPMI-1640 Complete Medium (10%FBS; Gibco) was used for culture. When the cells reached 80% confluence, they were passed on. 2.3. PBMCs Immune Reconstitution PBMCs were purchased from AllCells and processed according to the company'sFull Paper
2022Ryan, DE;Diamant-Levi, T;Steinfeld, I;Taussig, D;Visal-Shah, S;Thakker, S;Lunstad, BD;Kaiser, RJ;McCaffrey, R;Ortiz, M;Townsend, J;Welch, WRW;Singh, M;Curry, B;Dellinger, DJ;Bruhn, L;Phosphonoacetate Modifications Enhance the Stability and Editing Yields of Guide RNAs for Cas9 EditorsBiochemistry35436085Human primary T cells (LP, CR, CD3+, NS) were obtained from AllCells (Alameda, CA) and were cultured in RPMI 1640 plus GlutaMax media supplemented with 10% fetal bovine serum, 5 ng/mL human IL-7, and 5 ng/mL human IL-15 (Gibco). Primary T cells wereFull Paper
2022Darbaniyan, F;Zheng, H;Kanagal-Shamanna, R;Lockyer, P;Montalban-Bravo, G;Estecio, M;Lu, Y;Soltysiak, KA;Chien, KS;Yang, H;Sasaki, K;Class, C;Ganan-Gomez, I;Do, KA;Garcia-Manero, G;Wei, Y;Transcriptomic Signatures of Hypomethylating Agent Failure in Myelodysplastic Syndromes and Chronic Myelomonocytic LeukemiaExperimental hematology36150563CD34 + cells isolated from healthy individuals (AllCells, Alameda CA) and from patients with MDS or CMML were plated into human methylcellulose colony formation medium Methocult- SaveFull Paper
2022Valdez, BC;Yuan, B;Murray, D;Nieto, Y;Popat, U;Andersson, BS;Enhanced cytotoxicity of bisantrene when combined with venetoclax, panobinostat, decitabine and olaparib in acute myeloid leukemia cellsLeukemia & lymphoma35188042Bisantrene (Bis), a topoisomerase-II inhibitor, is less cardiotoxic than the current anthracyclines. Its synergistic cytotoxicity with newly developed antineoplastic drugs has not been SaveFull Paper
2022Fan, F;Liu, F;Shen, P;Tao, L;Zhang, H;Wu, H;Salvianolic acid B, a new type I IRE1 kinase inhibitor, abrogates AngII-induced angiogenesis by interacting with IRE1 in its active conformationClinical and experimental pharmacology & physiology36153795Primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China) and cultured in EBM media (AllCells ) according to the manufacturer's SaveFull Paper
2022Tong, Q;Liu, H;Qi, Q;Dai, C;Yang, T;Qian, F;Development of a fully human anti-GITR antibody with potent antitumor activity using H2L2 miceFEBS open bio35674216h well, and luminescence values were measured using a Varioskan LUX (Thermo, Carlsbad, CA, USA). T‐cell activation assays Total T cells from frozen human peripheral blood mononuclear cells (PBMCs, AllCells, Alameda, CA, USA) were first isolated usinFull Paper
2022Yang, M;Tkach, D;Boyne, A;Kazancioglu, S;Duclert, A;Poirot, L;Duchateau, P;Juillerat, A;Optimized two-step electroporation process to achieve efficient nonviral-mediated gene insertion into primary T cellsFEBS open bio34510816ineering in human primary cells and provide a foundation for further advances using nonviral gene editing methods. Materials and methods T‐cell culture Cryopreserved human PBMCs were acquired from ALLCELLS. PBMCs were cultured in X‐vivo‐15 media (LoFull Paper
2022Pieles, O;Reichert, TE;Morsczeck, C;Protein kinase A is activated during bone morphogenetic protein 2-induced osteogenic differentiation of dental follicle stem cells via endogenous parathyroid hormone-related proteinArchives of oral biology35338829Human dental follicle stem cells (Catalog number FT002F, Lot number ACF001) were commercially obtained from AllCells (Emeryville, USA) and cultured in Dulbecco's Modified Eagle's Medium (DMEM) high glucose (Sigma Aldrich, St. Louis, USA) with 10% fetFull Paper
2022Qian, J;Yu, J;Zhu, X;Liang, S;MiR-335 promotes corneal neovascularization by Targeting EGFRBMC ophthalmology35701740surgery and on day 3, 7, 11 and 14. The Animal Research Committee of the Affiliated Hospital of Nantong University approved all animal studies. Cell culture and different treatments HUVECs were from AllCells (Shanghai, China). They were put in an RPFull Paper
2022Wang, W;Tang, W;Shan, E;Zhang, L;Chen, S;Yu, C;Gao, Y;MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FASEuropean journal of histochemistry : EJH35638591cells (HUVEC) in vitro. Materials and Methods Cell culture HUVEC cells have played a major role as a model system for the study of the regulation of endothelial cell function. HUVEC cells (Shanghai AllCells Biotech Co., Ltd. Shanghai, China) were cuFull Paper
2022Koh, H;Zhen, X;Kim, J;Ha, HY;Lee, JH;Generation and characterization of human umbilical cord blood-derived induced pluripotent stem cells (KRIBBi005-A)Stem cell research35085946Ethical approval Approval obtained by Public Institutional Bioethics Committee designated by the MOHW (P01-201909-31-002). Cord blood mononuclear cells were purchased (AllCells LLC)Full Paper
2022Hashim, SNM;Azlina, A;Ultrastructural morphological changes of VEGF treated-stem cells from human exfoliated deciduous teeth cultured on human amniotic membraneActa MicroscopicaSHED (AllCells, USA) were cultured in a complete medium made of the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, USA), supplemented with 15% of fetal bovine serum (FBS) (Gibco, USA) and 50 U/ml of penicillin-stFull Paper
2022Bai, Y;Zhang, X;Zheng, J;Liu, Z;Yang, Z;Zhang, X;Overcoming high level adenosine-mediated immunosuppression by DZD2269, a potent and selective A2aR antagonistJournal of experimental & clinical cancer research : CR36229853bR, CHO-A1R and CHO-A3R) were purchased from GenScript. Cell lines RM-1, B16F10 and Pan02 were obtained from the American Type Culture Collection (ATCC). Cryopreserved human PBMCs were purchased from AllCells. All CHO cells were cultured in Ham’s F12Full Paper
2022Kartha, VK;Duarte, FM;Hu, Y;Ma, S;Chew, JG;Lareau, CA;Earl, A;Burkett, ZD;Kohlway, AS;Lebofsky, R;Buenrostro, JD;Functional inference of gene regulation using single-cell multi-omicsCell genomics36204155AILS Human peripheral blood mononuclear cells Cryopreserved human peripheral blood mononuclear cells (PBMCs) and isolated peripheral blood CD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from AllCells (see Table S1 for catalog numbers and donFull Paper
2022Riaz, S;Azlina, A;Mahmood, Z;Htun, AT;Long-term treatment of dentine with triple antibiotic paste promotes stem cell viability and attachmentJournal of Taibah University Medical Sciences35983454, the dentine chips were removed from the well plates and air-dried on a filter paper. The samples were then stored in a sterile container at 4 °C until further use. Cell culture Commercial DPSCs (AllCells, USA) were used from passage 7 to 9 at 70%-Full Paper
2022Salam, N;Gibson, IR;Lithium ion doped carbonated hydroxyapatite compositions: Synthesis, physicochemical characterisation and effect on osteogenic response in vitroBiomaterials advances35939955the American Type Culture Collection (via LGC standards, UK), PromoCell (Germany), and AllCells (USA), respectively. Cells were maintained in routine culture conditions using: high glucose DMEM with 10 % FBS and 4 mM l-glutamine for C2C12 cells, GibcFull Paper
2022Nakauchi, Y;Azizi, A;Thomas, D;Corces, MR;Reinisch, A;Sharma, R;Cruz Hernandez, D;Köhnke, T;Karigane, D;Fan, A;Martinez-Krams, D;Stafford, M;Kaur, S;Dutta, R;Phan, P;Ediriwickrema, A;McCarthy, E;Ning, Y;Phillips, T;Ellison, CK;Guler, GD;Bergamaschi, A;Ku, CJ;Levy, S;Majeti, R;The Cell Type-Specific 5hmC Landscape and Dynamics of Healthy Human Hematopoiesis and TET2-Mutant PreleukemiaBlood cancer discovery35532363Normal donor human BM cells were obtained fresh from AllCells, and peripheral blood cells Human BM samples (CD34+ cell enriched) were purchased from AllCells. Mononuclear SaveFull Paper
2022Baskar, R;Chen, AF;Favaro, P;Reynolds, W;Mueller, F;Borges, L;Jiang, S;Park, HS;Kool, ET;Greenleaf, WJ;Bendall, SC;Integrating transcription-factor abundance with chromatin accessibility in human erythroid lineage commitmentCell reports methods35463156# ab181544 Cleaved caspase 3-PE BD Biosciences Cat# 550821, RRID:AB_393906 ------------------------- Biological samples ------------------------- Adult bone marrow All Cells Inc https://allcells.com/research-grade-tissue-products/bone-mFull Paper
2022Kwak, J;Choi, W;Bae, Y;Kim, M;Choi, S;Oh, W;Jin, H;HLA-A2 Promotes the Therapeutic Effect of Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Hyperoxic Lung InjuryBioengineering (Basel, Switzerland)35447737St. Louis, MO, USA) for 1 h at 37 °C. The UCB-MSCs (1 × 103 cells/well) were seeded and maintained at 37 °C in a humidified incubator for 4 h and then co-cultured with PBMCs (1 × 105 cells/well; Allcells, Boston, MA, USA) from different donors. PhytoFull Paper
2022Vivanco Gonzalez, N;Oliveria, JP;Tebaykin, D;Ivison, GT;Mukai, K;Tsai, MM;Borges, L;Nadeau, KC;Galli, SJ;Tsai, AG;Bendall, SC;An optimized protocol for phenotyping human granulocytes by mass cytometrySTAR protocols35434655y donors (with unknown allergy status) were obtained from the Stanford Blood Center (Palo Alto, CA, USA). Healthy bone marrow and peripheral blood samples paired from the same donor were ordered from AllCells (Alameda, CA, USA). Samples from patientsFull Paper
2022Bell-Glenn, S;Thompson, JA;Salas, LA;Koestler, DC;A Novel Framework for the Identification of Reference DNA Methylation Libraries for Reference-Based Deconvolution of Cellular MixturesFrontiers in bioinformatics35419567Cell Mixture Reconstruction Experiment Purified cells taken from normal human subjects were purchased from AllCells LLC (Emeryville, CA). Namely, granulocytes, monocytes, CD4T, SaveFull Paper
2022Shui, S;Wang, S;Liu, J;Systematic Investigation of the Effects of Multiple SV40 Nuclear Localization Signal Fusion on the Genome Editing Activity of Purified SpCas9Bioengineering (Basel, Switzerland)35200436s medium (DMEM) supplemented with 10% (v/v) FBS, 100 U/mL penicillin and 100 U/mL streptomycin. The medium of EGFP reporter contained an additional 50 U/mL hygromycin. Human dermal fibroblasts (hDF, AllCells) were acquired from Cell Applications (LotFull Paper
2022Li, L;Xu, H;Gao, L;Doxorubicin-Loaded Black Phosphorus Multifunctional Nanodelivery System Combined with Photothermal Therapy Promotes Immunogenic Death of Prostate Cancer PC-3 CellsSSRN Electronic JournalBeijing, China). Dendritic cells (DCs) were 197obtained from AllCells (San Francisco, USA). FITC anti198human CD11c Antibody, Alexa Fluor® 647 anti-human 199CD80 Antibody, PE anti-human CD86 Antibody, and 200PerCP/Cyanine5.5 anti-human HLA-DR AntibodFull Paper
2022Calderon, A;Kim, Y;Favaro, P;Baskar, R;Valainis, J;Reynolds, W;Borges, L;Glass, D;Tsai, A;Bendall, S;Multi-Omic Single Cell Identification of Lin- CD34- Natural Killer Cell Progenitors in Human HematopoiesisSSRN Electronic JournalDeidentified human blood (n=5) and BM (n=7) were obtained from healthy adult donors (Stanford Blood Center, Allcells). All samples were obtained under informed consent and in SaveFull Paper
2022Lee, J;Jonus, H;Sadanand, A;Branella, G;Maximov, V;Suttapitugsakul, S;Schniederjan, M;Shim, J;Parwani, K;Fedanov, A;Pilgrim, A;Silva, J;Doering, C;Wu, R;Spencer, H;Goldsmith, K;Identification and Targeting of Protein Tyrosine Kinase 7 (PTK7) as an Immunotherapy Candidate for Neuroblastoma Cellular TherapySSRN Electronic JournalGD2-targeted immunotherapy improves survival for pediatric patients with neuroblastoma (NB). However, toxicities associated with on-target, off-tumor activity occur. Furthermore, GD2 SaveFull Paper
2022O'Brien, G;Cecotka, A;Manola, K;Pagoni, M;Polańska, J;Badie, C;Epigenetic Signature of Ionising Radiation In Therapy-Related AML PatientsSSRN Electronic JournalBone marrow aspirate samples were also obtained from 5 healthy normal donors (4 males, 1 female, age 21-33) from AllCells® (Alamada, USA). The project was in accordance with the declaration of Helsinki. Informed consent was provided from patients andFull Paper
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2022Li, W;Li, J;Hu, X;Xu, L;Liu, X;Qian, Z;Jin, L;Zhang, Y;Wei, J;Liu, X;Tumor suppression by Chinese cordyceps extract via anti-angiogenesisResearch SquareHuman umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in special medium for HUVEC cells at 37C with 5% CO2. CCE was SaveFull Paper
2022Li, L;Chen, X;Li, Y;Lou, L;Pharmacological characterization of GR1803, a novel BCMA × CD3 bispecific antibody for multiple myeloma treatmentResearch SquareThe MM cell lines NCI-H929, MM.1S, RPMI-8226, and U266 were obtained from the American Type Culture Collection (Manassas, VA, USA). Fresh peripheral blood mononuclear cells (PBMCs) were obtained from AllCells Co. (Shanghai, China). Natural killer (NKFull Paper
2022Yan, F;Luo, B;Chen, C;Tan, B;Cui, D;Wang, M;Qian, J;Inhibiting PHD2 in bone marrow mesenchymal stem cells in an inflammatory microenvironment facilitates periodontal repair in SD ratsResearch SquarePrimary BMMSCs of SD rats were isolated and puri ed by AllCells (Alameda, CA, USA), and their phenotype was identi ed. The cells were recovered and cultured in low glucose SaveFull Paper
2022Guedes, F;Branquinho, M;Biscaia, S;Alvites, R;Sousa, A;Lopes, B;Sousa, P;Rêma, A;Amorim, I;Faria, F;Patrício, T;Bugalho, A;Alves, N;Maurício, A;Gamma Irradiation Processing on 3D PCL Devices - A Preliminary Biocompatibility AssessmentpreprintsHuman Dental Pulp stem/stromal cells (hDPSCs) obtained from AllCells, LLC (Cat. DP0037F, Lot Nº DPSC090411-01) were maintained in MEM α, GlutaMAX™ Supplement, no nucleosides (Gibco, 32561029), supplemented with 10% (v/v) fetal bovine serum (FBS) (GibFull Paper
2022Tokatlian, T;Asuelime, G;Naradikian, M;Mock, J;Daris, M;Martin, A;Toledo Warshaviak, D;Kamb, A;Hamburger, A;Chimeric Antigen Receptors Directed at Mutant KRAS Exhibit an Inverse Relationship Between Functional Potency and Neoantigen SelectivityCancer Research CommunicationsPeripheral blood mononuclear cells (PBMC) from healthy donors were obtained from AllCells. PBMC collection and donor written informed consent were approved by an Institutional SaveFull Paper
2022Boccacci, Y;Dumont, N;Doyon, Y;Laganière, J;Accessory-cell-free differentiation of hematopoietic stem and progenitor cells into mature red blood cellsbioRxivThe culture and ex vivo engineering of red blood cells (RBCs) can help characterize genetic variants, model diseases, and may eventually spur the development of applications in SaveFull Paper
2022Checkley, M;Luttge, B;Dobrowolski, C;Wald, D;McMahon, D;Haidar, G;Sobolewski, M;Enick, P;Cyktor, J;Mellors, J;Karn, J;Reduction of the HIV-1 reservoir in T cells from persons with HIV-1 on suppressive antiretroviral therapy using expanded natural killer cells ex vivobioRxivFor this study leukapheresis packs from 5 HIV-1-negative donors were purchased from AllCells. 437 For HIV+ participants, PBMCs from well-suppressed ART-treated patients from the SaveFull Paper
2022Shabaneh, T;Moffett, H;Stull, S;Derezes, T;Tait, L;Park, S;Riddell, S;Lajoie, M;Safety switch optimization enhances antibody-mediated elimination of CAR T cellsbioRxivNatural killer cells were isolated from cryopreserved, T cell depleted (CD4-/CD8-) PBMC (AllCells) by negative selection using the EasySep Human NK Cell Kit (Stemcell) according to the manufacturer’s protocol. To activate their cytolytic function, isFull Paper
2022Wisdom, K;Suijker, J;van den Broek, L;Sridharan, B;Grandhi, T;Cheng, A;Lamb, M;Titus, S;Gehman, A;Poore, D;Shah, N;Cheng, S;Kim, E;Griffin, S;Ekert, J;Lung Tumor Microphysiological System with 3D Endothelium to Evaluate Modulators of T-Cell InfiltrationbioRxivAllCells and shipped to the MIMETAS research facility, or they 653 were isolated from AllCells For this, T-cells were isolated from full 654 fresh leukopaks (AllCells). Leukopaks were SaveFull Paper
2022Dogan, M;Karhan, E;Kozhaya, L;Placek, L;Chen, X;Yigit, M;Unutmaz, D;Engineering human Mucosal Associated Invariant T (MAIT) cells with chimeric antigen receptors for cancer immunotherapy1bioRxivHealthy adult blood was obtained from AllCells Quincy, MA. PBMCs were isolated using Ficoll- 100 paque plus (GE Health care). CD4+ T, CD8+ T, CD19+ B cells, CD14+ monocytes SaveFull Paper
2022Swift, M;Horns, F;Quake, S;Fate Bias and Transcriptional Memory of human B cellsbioRxivfrom a healthy male aged 50-55 were obtained from AllCells. Mononuclear cells were isolated using Ficoll gradient and Red Blood Cell Lysis. Plasma cells from the bone marrow were isolated using StemCell EasySepEasySep™ Human CD138 Positive SelectionFull Paper
2022White, B;de Reyniès, A;Newman, A;Waterfall, J;Lamb, A;Petitprez, F;Valdeolivas, A;Lin, Y;Li, H;Xiao, X;Wang, S;Zheng, F;Yang, W;Yu, R;Guerrero-Gimenez, M;Catania, C;Lang, B;Domanskyi, S;Bertus, T;Piermarocchi, C;Monaco, G;Caruso, F;Ceccarelli, M;Yu, T;Guo, X;Coller, J;Maecker, H;Duault, C;Shokoohi, V;Patel, S;Liliental, J;Simon, S;Saez-Rodriguez, J;Heiser, L;Guinney, J;Gentles, A;Tumor Deconvolution DREAM Challenge consortium, ;Community assessment of methods to deconvolve cellular composition from bulk gene expressionbioRxivImmune and stromal cells provided by StemExpress and AllCells were isolated according to ) and the rest assigned immune cells from AllCells wherever availability allowed (Tables S5 Save CachedFull Paper
2022Simmons, S;Lithwick-Yanai, G;Adiconis, X;Oberstrass, F;Iremadze, N;Geiger-Schuller, K;Thakore, P;Frangieh, C;Barad, O;Almogy, G;Rozenblatt-Rosen, O;Regev, A;Lipson, D;Levin, J;Single cell RNA-seq by mostly-natural sequencing by synthesisbioRxivWe purchased 9 cryopreserved human PMBC samples (AllCells). We thawed PBMC vials in a 37C water bath for ∼2 minutes. A quick counting revealed high viability (>90%) in all SaveFull Paper
2022Seenappa, L;Jakubowski, A;Steinbuck, M;Palmer, E;Haqq, C;Carter, C;Fontenot, J;Villinger, F;McNeil, L;DeMuth, P;Programming the lymph node immune response with Amphiphile-CpG induces potent cellular and humoral immunity following COVID-19 subunit vaccination in mice and non-human primatesbioRxivwho had recovered from SARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and ALLCELLS (Alameda, CA). All samples were received and stored frozen at −80°C until analysisFull Paper
2022Lutter, L;ter Linde, J;Brand, E;van Konijnenburg, D;Roosenboom, B;Talabur-Horje, C;Oldenburg, B;van Wijk, F;Compartment-driven imprinting of intestinal CD4 (regulatory) T cells in inflammatory bowel disease and homeostasisbioRxivCluster 1 (18.6% of allcells) was characterized by KLF2, SELL, CCR7, TCF7 and LEF1, and thus resembled recently migrated/recirculating T cells (figure 4A). This cluster contained both intraepithelial and lamina propria T cells (figure 4E). Similarly,Full Paper
2022Jaffe, D;Shahi, P;Adams, B;Chrisman, A;Finnegan, P;Raman, N;Royall, A;Tsai, F;Vollbrecht, T;Reyes, D;McDonnell, W;Functional antibodies exhibit light chain coherencebioRxivWe titrated and developed the B cell fractionation panel using 20 million fresh PBMCs (AllCells, catalog # 3050363) from a healthy human donor whose cells were not used to generate SaveFull Paper
2022Persad, S;Choo, Z;Dien, C;Masilionis, I;Chaligné, R;Nawy, T;Brown, C;Pe’er, I;Setty, M;Pe’er, D;SEACells: Inference of transcriptional and epigenomic cellular states from single-cell genomics databioRxivCryopreserved bone marrow stem/progenitor CD34+ cells from a healthy donor were purchased from AllCells, LLC. (catalog no. ABM022F) and stored in vapor phase nitrogen. Vial was removed from the storage and immediately thawed at 37 °C in a water bathFull Paper
2022Stuart, T;Hao, S;Zhang, B;Mekerishvili, L;Landau, D;Maniatis, S;Satija, R;Raimondi, I;Nanobody-tethered transposition allows for multifactorial chromatin profiling at single-cell resolutionbioRxivCryopreserved healthy donor PBMCs were isolated from mobilized peripheral blood, BMMCs were purchased from AllCells. After thawing into DMEM with 10% FBS, the cells were spun SaveFull Paper
2022MacMullan, M;Dunn, Z;Qu, Y;Wang, P;Graham, N;Phospho-proteomic analysis of CAR-T cell signaling following activation by antigen-presenting cancer cellsbioRxivcDNAs from BM CD34+, PB CD34+, BM MNCs, PB MNCs and BM MSCs purchased from AllCells (Emeryville, CA, USA) was obtained from a population of positive cells (purity exceeding 95%) collected from five individual donors. The level of cognate cDNA was meaFull Paper
2022Dogan, M;Kozhaya, L;Placek, L;Karabacak, F;Yigit, M;Unutmaz, D;Targeting SARS-CoV-2 infection through CAR-T like bispecific T cell engagers incorporating ACE2bioRxivHealthy adult blood was obtained from AllCells. PBMCs were isolated using Ficoll-paque plus (GE Health care). CD8 T cells were purified using Dynal CD8 Positive Isolation Kit (from SaveFull Paper
2022Yashar, W;Curtiss, B;Coleman, D;Van-Campen, J;Kong, G;Macaraeg, J;Estabrook, J;Demir, E;Long, N;Bottomly, D;McWeeney, S;Tyner, J;Druker, B;Maxson, J;Braun, T;Disruption of the MYC Super-Enhancer Complex by Dual Targeting of FLT3 and LSD1 in Acute Myeloid LeukemiabioRxivWhole bone marrow was obtained (AllCells) and CD34+ cells were selected using CD34 MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions. For the colony assay, 500 CD34+ cells were used per replicate and plated in MethoCult™ H4435Full Paper
2022Aksöz, M;Gafencu, G;Stoilova, B;Buono, M;Meng, Y;Jakobsen, N;Metzner, M;Clark, S;Beveridge, R;Thongjuea, S;Vyas, P;Nerlov, C;Identification and Age-dependent Increase of Platelet Biased Human Hematopoietic Stem CellsbioRxivFresh BM from healthy young volunteers (21 to 34 years old males) were purchased from AllCells (Berkeley, CA) or Lonza (Lonza Bioscience, Basel, Switzerland). Bone marrow from older donors was obtained from orthoplastic surgery afterFull Paper
2022Xiang Ping, M;Zhi, H;Aziz, N;Hadri, N;Ghazalli, N;Yusop, N;Optimization of agarose-alginate hydrogel bead components for encapsulation and transportation of stem cellsJournal of Taibah University Medical SciencesStem cells from human exfoliated deciduous teeth (SHED) purchased from AllCells USA were revived from the cryovial and maintained in culture before encapsulation. SHED from SaveFull Paper
2022Marcisak, EF;Leveraging single-cell genomics to uncover clinical and preclinical responses to cancer immunotherapyThesisGFP due to transduction with pBMN-IRES-EGFP containing the FcγRIIIA construct. All NK cell lines were cultured as previously described[231]. Fresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selectedFull Paper
2022Nesterenko, PA;Characterizing the T cell immune response through the receptor-ligand interactionThesisCell culture: Cryo preserved peripheral blood mononuclear cells (PBMCs) were commercially purchased (Allcells and Hemacare). PBMCs were thawed in a water bath set to 37C, transferred to 50 mL conical tube, 1 mL of warm R10 media was added drop wise aFull Paper
2022Chu, J;Ligament tissue engineering: assessing differentiation potential of dental follicle cells in a self-assembly three-dimensional organoid model.Thesisaccording to AllCells company regulations, further donor information such gender and exact age was not provided to the purchaser). The PDL-hTERT immortal cell line has already SaveFull Paper
2022Plasek, LM;Self-Silencing: An HIV Induced Mechanism for T Cell Quiescence and HIV LatencyThesisPeripheral blood mononuclear cells from HIV negative donors were purchased from AllCells. Naive CD4+ T cells were isolated using the EasySepTM Human Naive CD4+ T Cell SaveFull Paper
2022BOSYKH, D;Mechanisms and Biological Consequences of Mutual Reprogramming by Melanoma Cells and Normal Dermal FibroblastsThesisCell lines and medium Primary human neonatal dermal fibroblasts (NDFs; AllCells, LLC) were combined from three individual donors. NDFs were routinely tested for mycoplasma and confirmed to be negative using the MycoAlert mycoplasma detection kit (LonFull Paper
2022Oostindie, SC;Rinaldi, DA;Zom, GG;Wester, MJ;Paulet, D;Al-Tamimi, K;van der Meijden, E;Scheick, JR;Wilpshaar, T;de Jong, B;Hoff-van den Broek, M;Grattan, RM;Oosterhoff, JJ;Vignau, J;Verploegen, S;Boross, P;Beurskens, FJ;Lidke, DS;Schuurman, J;de Jong, RN;Logic-gated antibody pairs that selectively act on cells co-expressing two antigensNature biotechnology35879362PBMCs derived from patients with CLL were commercially obtained from Discovery Life Sciences. Characteristics of patients with CLL are summarized in Supplementary SaveFull Paper
2022Schuler, M;Cuppens, K;Ploenes, T;Vanbockrijck, M;Wiesweg, M;Darwiche, K;Schramm, A;Maes, B;Hegedus, B;Schildhaus, H;Hautzel, H;Theegarten, D;Baas, P;Hartemink, K;Du Pont, B;Aigner, C;LBA37 A randomized, multicentric phase II study of preoperative nivolumab plus relatlimab or nivolumab in patients with resectable non-small cell lung cancer (NEOpredict-Lung)Annals of Oncologycology, AstraZeneca, Eisai, Takeda, ZytoVision, Zytomed Systems, Molecular Health; Financial Interests, Institutional, Research Grant: Novartis Oncology; Financial Interests, Personal, Full or part-time Employment: Targos Molecular Pathology Inc.. H.Full Paper
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2022Lau, D;Khare, S;Stein, MM;Jain, P;Gao, Y;BenTaieb, A;Rand, TA;Salahudeen, AA;Khan, AA;Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancerNature communications35831288Samples for single-cell multi-omic sequencing were previously frozen dissociated tumor cells (DTCs) (Discovery Life Sciences, Huntsville, AL). DTCs were thawed and Save;xpression analyses were performed on log10-transformed counts. Single-cell mulFull Paper
2022Leung, DH;Devaraj, S;Goodrich, NP;Chen, X;Rajapakshe, D;Ye, W;Andreev, V;Minard, CG;Guffey, D;Molleston, JP;Bass, LM;Karpen, SJ;Kamath, BM;Wang, KS;Sundaram, SS;Rosenthal, P;McKiernan, P;Loomes, KM;Jensen, MK;Horslen, S;Bezerra, JA;Magee, JC;Merion, RM;Sokol, RJ;Shneider, BL;For ChiLDReN, ;Serum Biomarkers Correlated with Liver Stiffness Assessed in a Multi-center Study of Pediatric Cholestatic Liver DiseaseHepatology (Baltimore, Md.)36069569similar distributions of age- and sex-matched children with normal liver biochemistries and without known liver disease served as case controls (Discovery Life Sciences, SaveFull Paper
2022Yap, TA;Gainor, JF;Callahan, MK;Falchook, GS;Pachynski, RK;LoRusso, P;Kummar, S;Gibney, GT;Burris, HA;Tykodi, SS;Rahma, OE;Seiwert, TY;Papadopoulos, KP;Blum Murphy, M;Park, H;Hanson, A;Hashambhoy-Ramsay, Y;McGrath, L;Hooper, E;Xiao, X;Cohen, H;Fan, M;Felitsky, D;Hart, C;McComb, R;Brown, K;Sepahi, A;Jimenez, J;Zhang, W;Baeck, J;Laken, H;Murray, R;Trehu, E;Harvey, CJ;First-in-Human Phase I/II ICONIC Trial of the ICOS Agonist Vopratelimab Alone and with Nivolumab: ICOS-High CD4 T-Cell Populations and Predictors of ResponseClinical cancer research : an official journal of the American Association for Cancer Research35511938Full Paper
2022Shalev, TJ;Gamal El-Dien, O;Yuen, MMS;Shengqiang, S;Jackman, SD;Warren, RL;Coombe, L;van der Merwe, L;Stewart, A;Boston, LB;Plott, C;Jenkins, J;He, G;Yan, J;Yan, M;Guo, J;Breinholt, JW;Neves, LG;Grimwood, J;Rieseberg, LH;Schmutz, J;Birol, I;Kirst, M;Yanchuk, AD;Ritland, C;Russell, JH;Bohlmann, J;The western redcedar genome reveals low genetic diversity in a self-compatible coniferGenome research36109148University of British Columbia; tal.shalev@msl.ubc.ca.++University of British Columbia.++University of British Columbia.++Department of Energy Joint Genome Institute.++Canada's Michael Smith Genome Sciences Centre.++Canada's Michael Smith Genome ScieFull Paper
2022Cramer, DAT;Franc, V;Caval, T;Heck, AJR;Charting the Proteoform Landscape of Serum Proteins in Individual Donors by High-Resolution Native Mass SpectrometryAnalytical chemistry36074704ditions. Experimental Procedures Individual serum samples from six healthy donors were provided by Sanquin Research (Amsterdam, The Netherlands). Serum samples of diseased donors were purchased from Discovery Life Sciences (Columbus, OH, USA). FurthFull Paper
2022Pawley, DC;Dikici, E;Deo, SK;Raccamarich, P;Fischl, MA;Alcaide, M;Daunert, S;Rapid Point-of-Care Test Kit for Bacterial Vaginosis: Detection of Vaginolysin and Clue Cells Using Paper Strips and a SmartphoneAnalytical chemistry35943181Purchased patient samples from Discovery Life Sciences confirmed positive for BV were also tested. All patient samples tested showed elevated levels of VLY, indicating a SaveFull Paper
2022Nuti, S;Zhang, Y;Zerrouki, N;Roach, C;Bänfer, G;Kumar, GL;Manna, E;Diezko, R;Kersch, K;Rüschoff, J;Jasani, B;High interobserver and intraobserver reproducibility among pathologists assessing PD-L1 CPS across multiple indicationsHistopathology35993150Diezko is an employee of Targos Molecular Pathology GmbH, which performed pharmaceutical industry-sponsored professional training for pathologists and clinical trial Save;Biomarkers and Diagnostics, Oncology, Global Medical and Scientific Affairs,Full Paper
2022Loibl, S;Huang, CS;Mano, MS;Mamounas, EP;Geyer, CE;Untch, M;Thery, JC;Schwaner, I;Limentani, S;Loman, N;Lübbe, K;Chang, JC;Hatschek, T;Tesarowski, D;Song, C;Lysbet de Haas, S;Boulet, T;Lambertini, C;Wolmark, N;Adjuvant trastuzumab emtansine in HER2-positive breast cancer patients with HER2-negative residual invasive disease in KATHERINENPJ breast cancer36117201es obtained from the pretreatment primary tumor biopsy material (or residual tumor tissue from definitive surgery post-NAST). HER2 status of all samples was centrally assessed in the same laboratory (Targos Molecular Pathology, GmbH [Kassel, Germany]Full Paper
2022Seok, Y;Yin, Q;Li, R;Mauk, M;Bai, H;Bau, H;Manually-operated, slider cassette for multiplexed molecular detection at the point of careSensors and Actuators B: ChemicalHCV genotype 1 plasma sample (CV900106678051314DD, 69,000,000 IU/mL) for clinical research was purchased from Discovery life science Inc. (Huntsville, AL, USA)Full Paper
2022Singampalli, KL;Li, J;Lillehoj, PB;Rapid magneto-enzyme-linked immunosorbent assay for ultrasensitive protein detectionAnalytica chimica acta36038242falciparum infection obtained in Uganda under IRB/EC approval for general research use were purchased from Discovery Life Sciences. All human samples were de- Save; Blood samples from donors with P. falciparum infection obtained in Uganda under IRB/Full Paper
2022Koehler, VF;Adam, P;Fuss, CT;Jiang, L;Berg, E;Frank-Raue, K;Raue, F;Hoster, E;Knösel, T;Schildhaus, HU;Negele, T;Siebolts, U;Lorenz, K;Allelein, S;Schott, M;Spitzweg, C;Kroiss, M;German Study Group for Rare Malignant Tumors of the Thyroid and Parathyroid Glands, ;Treatment of RET-Positive Advanced Medullary Thyroid Cancer with Multi-Tyrosine Kinase Inhibitors-A Retrospective Multi-Center Registry AnalysisCancers35884466US is an employee of Targos Molecular Pathology, Inc., and received honoraria from or served as an advisory board member at Roche, Novartis Oncology, MSD, BMS, Save CachedFull Paper
2022Molina, SA;Davies, SJ;Sethi, D;Oh, S;Durand, N;Scott, M;Davies, LC;Wormuth, K;Clarke, D;Particulates are everywhere, but are they harmful in cell and gene therapies?Cytotherapy36175323International Society for Cell & Gene Therapy Process Development, Manufacturing, and Analytics Committee, Vancouver, Canada. Electronic address: sam.molina@gmail.com.++International Society for Cell & Gene Therapy Process Development, Manufacturing,Full Paper
2022Ren, Z;Chen, S;Qin, X;Li, F;Guo, L;Study of the roles of cytochrome P450 (CYPs) in the metabolism and cytotoxicity of perhexilineArchives of toxicology36083301Perhexiline is a prophylactic antianginal agent developed in the 1970s. Although, therapeutically, it remained a success, the concerns of its severe adverse effects Save;- Primary human hepatocytes (pooled from 10 donors) were obtained from DiscFull Paper
2022Cheng, LY;Dai, P;Wu, LR;Patel, AA;Zhang, DY;Direct capture and sequencing reveal ultra-short single-stranded DNA in biofluidsiScience36147958N = 6 plasma samples from diseased individuals (3 from Type 1 Diabetes (T1D) patients and 3 from Alzheimer’s disease (AD) patients) were acquired from BioIVT’s biorepository and shipped frozen. Animal total blood samples were purchased from DiscoveryFull Paper
2022Kemper, K;Gielen, E;Boross, P;Houtkamp, M;Plantinga, T;de Poot, S;Burm, S;Janmaat, M;Koopman, L;van den Brink, E;Rademaker, R;Verzijl, D;Engelberts, P;Satijn, D;Sasser, A;Breij, E;Mechanistic and pharmacodynamic studies of DuoBody-CD3x5T4 in preclinical tumor modelsLife Science AllianceDissociated tumor cells (DTCs) were procured from commercial vendors (Prisma Health/KIYATEC Biorepository or Discovery Life Sciences) who maintain strict ethical compliance, including fullyFull Paper
2022Rüschoff, J;Friedrich, M;Nagelmeier, I;Kirchner, M;Andresen, LM;Salomon, K;Portier, B;Sredni, ST;Schildhaus, HU;Jasani, B;Grzelinski, M;Viale, G;Comparison of HercepTest™ mAb pharmDx (Dako Omnis, GE001) with Ventana PATHWAY anti-HER-2/neu (4B5) in breast cancer: correlation with HER2 amplification and HER2 low statusVirchows Archiv : an international journal of pathology35970977in HER2 evaluation, having served over the past 20 years as readers in most of the trastuzumab, pertuzumab, and T-DM1 approval BC studies by Targos GmbH (Kassel, Save;Targos - A Discovery Life Sciences Company, Germaniastraße 7, 34119, Kassel, GermFull Paper
2022Suwa, R;Kume, Y;Kawase, M;Chishiki, M;Ono, T;Norito, S;Sato, K;Okamoto, M;Kumaki, S;Nagai, Y;Hosoya, M;Takeda, M;Nishimura, H;Hashimoto, K;Shirato, K;Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in JapanPathogens (Basel, Switzerland)35889999To confirm amplification specificity, we used pharyngeal, nasopharyngeal, and nasal swabs from Discovery Life Sciences (Los Osos, CA, USA) that were obtained for the Save CachedFull Paper
2022Rodrigues, D;van Kampen, R;van Bodegraven, AA;Kleinjans, JCS;Jennen, DGJ;de Kok, TM;Gene expression responses reflecting 5-FU-induced toxicity: Comparison between patient colon tissue and 3D human colon organoidsToxicology letters36183961All patients provided written informed consent. Human colon tissue samples were obtained by Boehringer Ingelheim Pharmaceuticals Inc. (Ridgefield, Connecticut, USA) from Discovery Life Sciences (Huntsville, Alabama, USA; formerly, Conversant BiologicFull Paper
2022Cao, L;Ledeboer, A;Pan, Y;Lu, Y;Meyer, K;Clinical enrollment assay to detect preexisting neutralizing antibodies to AAV6 with demonstrated transgene expression in gene therapy trialsGene therapy35778500Individual human serum samples were purchased from BioIVT (Westbury, NY, USA), Discovery Life Sciences (Los Osos, CA, USA), and Golden West Biologicals (Temecula, CA, USA)Full Paper
2022Zhang, L;Wang, Y;Homan, KT;Gaudette, SM;McCluskey, AJ;Chan, Y;Murphy, J;Abdalla, M;Nelson, CM;Sun, VZ;Erickson, JE;Knight, HL;Clabbers, A;Sterman, AJS;Mitra, S;Imaging the Alternatively Spliced D Domain of Tenascin C in a Preclinical Model of Inflammatory Bowel DiseaseMolecular imaging and biology35906512Clinical IBD colon was evaluated using a biotinylated anti-TNC D antibody. Frozen human tissue was obtained from CHTN (National Cancer Institute, Bethesda, MD), Folio Biosciences (Columbus, OH), and UMASS Memorial Gastroenterology (Worcester, MA)Full Paper
2022Holmberg, JA;Henry, SM;Burnouf, T;Devine, D;Marschner, S;Boothby, TC;Burger, SR;Chou, ST;Custer, B;Blumberg, N;Siegel, DL;Spitalnik, SL;National Blood Foundation 2021 Research and Development summit: Discovery, innovation, and challenges in advancing blood and biotherapiesTransfusion36169155consulting firm, and one of our clients is Discovery Life Sciences, an, apheresis services provider. My advice to DLS is the same as what I say in this manuscript. HemaCare SaveFull Paper
2022Edmonds, NL;Flores, SE;Mahmutovic, A;Young, SJ;Mauldin, IS;Slingluff, CL;CD103 and periplakin are potential biomarkers for response of metastatic melanoma to pembrolizumabMelanoma research36169985QualTek provided a modified proportion score indicating the percent of PD-L1 Qualtek also reported an overall estimate of the presence/absence of tumor-infiltrating SaveFull Paper
2022Kandi, S;Savaryn, JP;Ji, QC;Jenkins, GJ;Use of In-Sample Calibration Curve approach for quantification of peptides with high resolution Mass SpectrometryRapid communications in mass spectrometry : RCM35940586Human Peripheral Blood Mononuclear Cells (hPBMCs) were purchased from Discovery Life Sciences (Newtown, PA, USA)Full Paper
2022Boillat, MA;Hauser, PC;CO2-measuring dongleHardwareX3587373411 2.11 Mouser.com semiconductor U4 Sensirion SCD41-D-R1 1 45.66 45.66 Mouser.com CO2-sensor J1 GCT USB3505-30-A-KIT 1 2.35 2.35 Mouser.com Micro B USB 2.0 socket Qualtek 3025033-1 1 2.97 2.97 Mouser.comFull Paper
2022Schöniger, S;Rüschoff, J;Mismatch Repair Deficiency and Microsatellite InstabilityEncyclopediaTargos—A Discovery Life Sciences Company, Germaniastrasse 7, 34119 Kassel, GermanyFull Paper
2022Li, W;Li, Y;Xu, J;Chen, C;Gu, Y;Lu, Z;Li, D;Li, J;Sorg, A;Roberts, C;Mahajan, S;Gallant, M;Taggart, D;Multimodal Epigenetic Sequencing Analysis (MESA) of Cell-free DNA for Non-invasive Cancer DetectionResearch SquareCohort 1 subjects were recruited at clinical sites within the United States through the ELITE Study (NCT05181826) or were obtained through the following contract research organizations: BioIVT (Westbury, NY), BioOptions (Brea, CA), Discovery Life SciFull Paper
2022Koerner, L;Yang, T;Schmiel, M;Peifer, M;Buettner, R;Pasparakis, M;NEMO- and RelA-dependent NF-?B Signaling Promotes Small Cell Lung CancerbioRxivSmall cell lung cancer (SCLC) is an aggressive type of lung cancer driven by combined loss of the tumor suppressors RB1 and TP53. SCLC is highly metastatic and Save CachedFull Paper
2022Hebda-Bauer, E;Hagenauer, M;Blandino, P;Meng, F;Chitre, A;Ozel, A;Arakawa, K;Flagel, S;Watson, S;Palmer, A;Li, J;Akil, H;Transcriptional Profiling of the Hippocampus in an F2 Cross of a Genetic Rat Model of Internalizing vs. Externalizing Behavior and Addiction LiabilitybioRxivHudsonAlpha Discovery provided a variety of metrics for evaluating sample RNA quality, including RNA concentration (RNAconc), RNA integrity number (RIN), the Save CachedFull Paper
2022Chitre, A;Hebda-Bauer, E;Blandino, P;Bimschleger, H;Nguyen, K;Maras, P;Li, F;Ozel, A;Polysskaya, O;Cheng, R;Flagel, S;Watson, S;Li, J;Akil, H;Palmer, A;Genome-Wide Association Study in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like TraitsbioRxivFor these 20 rats, liver tissue was sent to HudsonAlpha Discovery (Discovery Life Sciences) for HMW genomic DNA extraction and long-range and deep sequencing. SaveFull Paper
2022Dejanovic, B;Wu, T;Tsai, M;Graykowski, D;Gandham, V;Rose, C;Bakalarski, C;Ngu, H;Wang, Y;Pandey, S;Rezzonico, M;Friedman, B;Edmonds, R;De Mazière, A;Rakosi-Schmidt, R;Singh, T;Klumperman, J;Foreman, O;Chang, M;Xie, L;Sheng, M;Hanson, J;Complement C1q-dependent excitatory and inhibitory synapse elimination by astrocytes and microglia in Alzheimer’s disease mouse modelsNature AgingCSF from patients with AD and healthy controls was obtained from Folio Biosciences and Precision Medicine with ethics committee approval and written informed consentFull Paper
2022Cady, J;Greytak, E;Whole-genome sequencing of degraded DNA for investigative genetic genealogyForensic Science International: Genetics Supplement SeriesSamples were submitted to either HudsonAlpha Discovery or Arbor Biosciences for library preparation and sequencing. For each sample, a small amount of the prepared SaveFull Paper
2022Newman, L;Useckaite, Z;Rowland, A;Addressing MISEV guidance using targeted LC?MS/MS: A method for the detection and quantification of extracellular vesicle?enriched and contaminant protein markers from bloodJournal of Extracellular BiologySerum and plasma samples from patients with non-alcoholic fatty liver disease (NAFLD) were purchased from Discovery Life Sciences (Hunstville, AL, USA). SaveFull Paper
2022D'ANTONIO, L;Esposizione a paracetamolo e acido sulfosalicilico: effetti biologici nel bivalve Mytilus spp.ThesisFrancisca Piedade, Sofia Bio, Bruno Nunes. 2020 Effects of common pharmaceutical drugsFull Paper
2022Wagner, RS;Environmental Factors Affecting Rhizophydiales Sp. Infecting Planktothrix Spp.Thesiswere then sent to HudsonAlpha Discovery Life Sciences (Hudson, AL USA) for sequencing. Sequencing and QC was done at HudsonAlpha Discovery Life Sciences using Save;- then sent to HudsonAlpha Discovery Life Sciences (Hudson, AL USA) for sequencinFull Paper
2022Ferreira Garcia Rodrigues, D;Gut feelingsmaastricht universityHuman healthy colon organoids were derived from a tissue biopsy collected from a healthy male donor (67 years old) and kindly provided by Boehringer Ingelheim Pharmaceuticals Inc. (Ridgefield, USA), who purchased tissue biopsies from Discovery Life SFull Paper
2022Fang, G;The use of perfusion MRI using ferumoxytol and small molecular weight gadolinium (Gd) agents to assess response to pembrolizumab in brain metastases and systemic lesions in NSCLC: A comparison of imaging modalities to address brain metastases, pseudoprogression and systemic lesion tumor flare (neuro-check pilot)ThesisTissue samples will be sent to QualTek Molecular Laboratories for PD-L1 biomarker Brain Tissue samples will also be sent to QualTek Molecular Laboratories for PD-1 SaveFull Paper
2022Isakoff, SJ;DF/HCC Protocol#: 17-328 TITLE: A Phase 1b Study of Safety and Immune Response to PVX-410 Vaccine Alone and in Combination with Pembrolizumabwebctportal.partners.orgPrimary tumor slides will be evaluated for expression of PD-L1 using the Merck Preferred Vendor QualTek according to their standard procedures. AFull Paper
2022Frankovi?, J;Koncerti hrvatskih autora za violon?elo i orkestarThesisMakedonskog teatra te je osniva? komornog ansambla za suvremenu glazbu „Sveta Sofia“. Bio je jedan od najplodnijih jugoslavenskih skladatelja srednje generacije te se okušao u svim stvarala?kim sferamaFull Paper
2022Yang, SYC;The Molecular Determinants of Response to Immune Checkpoint Therapy in Solid TumorsThesison 4-5?m sections mounted on positively charged ProbeOn slides (QualTek, Goleta, CA). QualTek provided a modified proportion score (MPS) indicating the proportion of SaveFull Paper
2022Shen, Y;Guo, K;Ma, A;Huang, Z;Du, J;Chen, J;Lin, Q;Wei, C;Wang, Z;Zhang, F;Zhang, J;Lin, W;Feng, N;Ma, W;Mitochondrial toxicity evaluation of traditional Chinese medicine injections with a dual in vitro approachFrontiers in pharmacology36408232and Health Sciences, Wuyi University, Jiangmen, China 3 Antibody Engineering Laboratory, School of Life Science & Technology, China Pharmaceutical University, Nanjing, China Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States ReviewFull Paper
2022Tiu, C;Lopez, J;Lisavanbulin. Microtubule destabilizer (tubulin polymerization inhibitor), Tumor checkpoint controller, Treatment of advanced glioblastoma multiformeDrugs of the FutureAssessment of 108 clinical trial samples from NCT02490800, using a CE-marked immunohistochemistry Clinical Trial Assay (CTA; Targos Molecular Pathology GmbH, SaveFull Paper
2022Drapkin, R;Jung, E;Bradshaw, C;Demars, L;Mosher, R;EP014/#408?Evaluation of NAPI2B expression in a well annotated longitudinal tissue series of ovarian serous carcinomasE-PostersIHC analyses were performed by QualTek Molecular Laboratories (Discovery Life Sciences). Editorial support for this poster was provided by BluPrint Oncology. SaveFull Paper
2022Korner, LK;Cell death and inflammation in skin homeostasis and small cell lung cancerThesisR.B. is an employee of Targos Molecular Pathology. The other authors declare no competing interestsFull Paper
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2022Canals Hernaez, D;Hughes, MR;Li, Y;Mainero Rocca, I;Dean, P;Brassard, J;Bell, EM;Samudio, I;Mes-Masson, AM;Narimatsu, Y;Clausen, H;Blixt, O;Roskelley, CD;McNagny, KM;Targeting a Tumor-Specific Epitope on Podocalyxin Increases Survival in Human Tumor Preclinical ModelsFrontiers in oncology35600398Linker stability was independently assessed by ReachBio Research Labs (RRL, www.reachbio.com) using an in vitro neutrophil differentiation killing assay, where extracellular Save;Emer Clarke at ReachBio Research Labs (www.reachbio.com) for providinFull Paper
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2022Vujovic, A;de Rooij, L;Chahi, A;Chen, H;Yee, B;Loganathan, S;Liu, L;Chan, D;Tajik, A;Tsao, E;Moreira, S;Joshi, P;Xu, J;Wong, N;Zandi, S;Aigner, S;Dick, J;Minden, M;Schramek, D;Yeo, G;Hope, K;A two-step in vivo CRISPR screen unveils pervasive RNA binding protein dependencies for leukemic stem cells and identifies ELAVL1 as a therapeutic targetbioRxivThawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio), supplemented with DMSO or 1.1uM DHTS. Colonies were scored on days 10-14. Human CB samples wereFull Paper
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2021Schneider, BP;Jiang, G;Ballinger, TJ;Shen, F;Chitambar, C;Nanda, R;Falkson, C;Lynce, FC;Gallagher, C;Isaacs, C;Blaya, M;Paplomata, E;Walling, R;Daily, K;Mahtani, R;Thompson, MA;Graham, R;Cooper, ME;Pavlick, DC;Albacker, LA;Gregg, J;Solzak, JP;Chen, YH;Bales, CL;Cantor, E;Hancock, BA;Kassem, N;Helft, P;O'Neil, B;Storniolo, AMV;Badve, S;Miller, KD;Radovich, M;BRE12-158: A Postneoadjuvant, Randomized Phase II Trial of Personalized Therapy Versus Treatment of Physician's Choice for Patients With Residual Triple-Negative Breast CancerJournal of clinical oncology : official journal of the American Society of Clinical Oncology34910554Speakers' Bureau: Genomic Health, Targos Molecular Pathology, AgilentFull Paper
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2021Voors-Pette, C;Aalders, W;Brill, M;Ferrari, D;Annis, A;Steidl, U;Aivado, M;Vukovic, V;1791P A phase I pharmacology study of the dual MDMX/MDM2 inhibitor, ALRN 6924, in healthy volunteersAnnals of OncologyWe thank the subjects who participated in these studies; Discovery Life Sciences in Newtown, PA; and Eric Smith for graphical assistanceFull Paper
2021Han, GR;Jang, H;Ki, H;Lee, H;Kim, MG;Reagent Filming for Universal Point-of-Care DiagnosticsSmall methods34928024A/B were purchased from Discovery Life Sciences (Los Osos, CA). TFull Paper
2021de Klerk, LK;Patel, AK;Derks, S;Pectasides, E;Augustin, J;Uduman, M;Raman, N;Akarca, FG;McCleary, NJ;Cleary, JM;Rubinson, DA;Clark, JW;Fitzpatrick, B;Brais, LK;Cavanaugh, ME;Rode, AJ;Jean, MG;Lizotte, PH;Nazzaro, MJ;Severgnini, M;Zheng, H;Fuchs, CS;Enzinger, PC;Bass, AJ;Phase II study of pembrolizumab in refractory esophageal cancer with correlates of response and survivalJournal for immunotherapy of cancer34593617PD-L1 immunohistochemistry was performed on pretreatment FFPE tissue slides using a laboratory developed assay (QualTek Molecular Laboratories, Newtown, Pennsylvania, USA) with the anti-PD-L1 22C3 antibody (Merck & Co, Palo Alto, California, USA).Full Paper
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2021Binnewies, M;Pollack, JL;Rudolph, J;Dash, S;Abushawish, M;Lee, T;Jahchan, NS;Canaday, P;Lu, E;Norng, M;Mankikar, S;Liu, VM;Du, X;Chen, A;Mehta, R;Palmer, R;Juric, V;Liang, L;Baker, KP;Reyno, L;Krummel, MF;Streuli, M;Sriram, V;Targeting TREM2 on tumor-associated macrophages enhances immunotherapyCell reports34686340Human tumor samples used for both flow cytometry and single-cell RNA sequencing were acquired from Discovery Life Sciences. 49 human tumor samples were used for flow cytometric analysis of immune composition and TREM2 surface levels. 10 human tumorFull Paper
2021Ma, J;Tan, X;Kwon, Y;Delgado, ER;Zarnegar, A;DeFrances, MC;Duncan, AW;Zarnegar, R;A Novel Humanized Model of NASH and Its Treatment With META4, A Potent Agonist of METCellular and molecular gastroenterology and hepatology34756982Background & Aims Nonalcoholic fatty liver disease is a frequent cause of hepatic dysfunction and is now a global epidemic. This ailment can progress to an advanced form called nonalcoholic steatohepatitis (NASH) and end-stage liver disease. CurrenFull Paper
2021Bassez, A;Vos, H;Van Dyck, L;Floris, G;Arijs, I;Desmedt, C;Boeckx, B;Vanden Bempt, M;Nevelsteen, I;Lambein, K;Punie, K;Neven, P;Garg, AD;Wildiers, H;Qian, J;Smeets, A;Lambrechts, D;A single-cell map of intratumoral changes during anti-PD1 treatment of patients with breast cancerNature medicine33958794combined with an intensity score, which represents the average intensity of positive tumor cells (0, none; 1, weak, 2, intermediate; 3, strong). PD-L1 (Merck 22C3 antibody) immunohistochemistry was performed by Qualtek Molecular Laboratories as per aFull Paper
2021Yuki, S;Nakamura, Y;Taniguchi, H;Denda, T;Nishina, T;Hamamoto, Y;Hara, H;Esaki, T;Kawakami, H;Takashima, A;Satoh, T;Sunakawa, Y;Masuishi, T;Shinozaki, E;Moriwaki, T;Miki, I;Shitara, K;Yoshino, T;Expression of PD-L1 and PD-L2 in colorectal cancer (CRC): A post-hoc integrated analysis of SCRUM-Japan GI-SCREEN CRC.Journal of Clinical OncologyPatients (Pts) with MSI-H or BRAF V600E mutant tumors were prioritized to be included. PD-L1 (22C3) and PD-L2 expressions were centrally assessed using immunohistochemical assays at QualTek and NeoGenomics, respectivelyFull Paper
2021Ho, CSL;Tüns, AI;Schildhaus, HU;Wiesweg, M;Grüner, BM;Hegedus, B;Schuler, M;Schramm, A;Oeck, S;HER2 mediates clinical resistance to the KRASG12C inhibitor sotorasib, which is overcome by co-targeting SHP2European journal of cancer (Oxford, England : 1990)34715459HUS is an employee of Targos Molecular Pathology, Inc.; received research grants (outside this study) from Novartis Oncology; and received honoraria and advisory board services from MSD, BMS, Roche Pharma, Novartis Oncology, ZytoVision, Eisai and PFull Paper
2021Israeli, S;Golden, A;Atalig, M;Mekki, N;Rais, A;Storey, H;Barbouche, MR;Peck, R;A Novel Point-of-Care Rapid Diagnostic Test for Screening Individuals for Antibody DeficienciesJournal of clinical immunology34839430These thirty-three specimens were acquired from Discovery Life Sciences (Los Osos, CA, USA) with IgG concentration characterized by Siemens BN II System nephelometer (Siemens Healthcare GmbH, Erlangen, Germany). IgG levels in the chosen samples SaFull Paper
2021Cheng, Y;Hall, TR;Xu, X;Yung, I;Souza, D;Zheng, J;Schiele, F;Hoffmann, M;Mbow, ML;Garnett, JP;Li, J;Targeting uPA-uPAR interaction to improve intestinal epithelial barrier integrity in inflammatory bowel diseaseEBioMedicine34933179anoids isolation and culture Isolation and culture of primary intestinal organoids was performed as previously described.2223 Briefly, human small intestinal tissues (obtained from Coversant Bio, now Discovery Life Sciences, https://www.dls.com/biospFull Paper
2021Cheng, L;Creasy, T;Pilataxi, F;Greenlees, L;Vence, L;Sridhar, S;Streicher, K;Effects of combination treatment with durvalumab plus tremelimumab on the tumor microenvironment in non-small-cell lung carcinomaCancer immunology, immunotherapy : CII34623465Frozen NSCLC tumor digests (untreated patients, three squamous cell carcinomas and one adenocarcinoma) were purchased from Discovery Life Sciences and cultured in 96-well plates in a medium containing low-dose interleukin 2 (IL-2) plus 20 µg/mL D aFull Paper
2021Bröske, AE;Korfi, K;Belousov, A;Wilson, S;Ooi, CH;Bolen, CR;Canamero, M;Alcaide, EG;James, I;Piccione, EC;Carlile, DJ;Dimier, N;Umaña, P;Bacac, M;Weisser, M;Dickinson, M;Pharmacodynamics and molecular correlates of response to glofitamab in relapsed/refractory non-Hodgkin lymphomaBlood advances34941996Biopsies were sent to the central pathology laboratory (Targos Molecular Pathology GmbH, Kassel, Germany) and fresh tissues were embedded on receipt. All biopsies were quality checked by a pathologist on hematoxylin and eosin (H&E) stainingFull Paper
2021Howard, FM;Villamar, D;He, G;Pearson, AT;Nanda, R;The emerging role of immune checkpoint inhibitors for the treatment of breast cancerExpert opinion on investigational drugs34569400PD-L1 status was initially assessed with the QualTek PD-L1 assay and subsequently with the Dako 22C3 PD-L1 assay (with With re-analysis of PD-L1 status, seven patients who had tested positive with the QualTek assay were negative by the 22C3 assayFull Paper
2021Manos, J;Preiss, C;Venkat, N;Tamm, J;Reinhardt, P;Kwon, T;Wu, J;Winter, A;Jahn, T;Yanamandra, K;Titterton, K;Karran, E;Langlois, X;UNCOVERING SPECIFICITY OF ENDOGENOUS TAU AGGREGATION IN A HUMAN IPSC-NEURON TAU SEEDING MODELiScience(Braak stage V/VI) and non-AD (Braak stage 0/I) male and female donors used in preparing sarkosyl-insoluble tau seeds were generously provided by the Massachusetts Alzheimer's Disease Research Center or purchased from Folio (now Discovery Life ScieFull Paper
2021Da Mesquita, S;Papadopoulos, Z;Dykstra, T;Brase, L;Farias, FG;Wall, M;Jiang, H;Kodira, CD;de Lima, KA;Herz, J;Louveau, A;Goldman, DH;Salvador, AF;Onengut-Gumuscu, S;Farber, E;Dabhi, N;Kennedy, T;Milam, MG;Baker, W;Smirnov, I;Rich, SS;Dominantly Inherited Alzheimer Network, ;Benitez, BA;Karch, CM;Perrin, RJ;Farlow, M;Chhatwal, JP;Holtzman, DM;Cruchaga, C;Harari, O;Kipnis, J;Meningeal lymphatics affect microglia responses and anti-Aβ immunotherapyNature33911285FC-404-2001) cartridges. Processing of total RNA extracted from mouse meningeal LECs (including linear RNA amplification and cDNA library generation) and RNA-seq was performed by HudsonAlpha Genomic Services Laboratory. For bulk RNA-seq experiments wFull Paper
2021Matusz-Fisher, A;Tan, AR;Combination of HER2-targeted agents with immune checkpoint inhibitors in the treatment of HER2-positive breast cancerExpert opinion on biological therapy34806498PD-L1-positivity was defined as staining in ≥ 1% of tumor cells or any staining in the stroma using the QualTek immunohistochemistry (IHC) assay. Of note, the assay was changed to the Dako IHC 22C3 pharmDx Q 2 Solutions assay mid-trial due to the pFull Paper
2021Luk, KC;Gersch, J;Harris, BJ;Holzmayer, V;Mbanya, D;Sauleda, S;Rodgers, MA;Cloherty, G;More DNA and RNA of HBV SP1 splice variants are detected in genotypes B and C at low viral replicationScientific reports34903774genotyping through the Abbott Global Surveillance Program38. All other plasma specimens sourced from France, Germany, Italy, Spain and Vietnam with known viral loads and genotypes were purchased from Discovery Life Sciences (Huntsville, AL). HBV DNAFull Paper
2021Connolly, P;Stapleton, S;Mosoyan, G;Fligelman, I;Tonar, YC;Fleming, F;Donovan, MJ;Analytical validation of a multi-biomarker algorithmic test for prediction of progressive kidney function decline in patients with early-stage kidney diseaseClinical proteomics34789168one specimens collected from patients receiving treatment with biologic therapeutic TNF-α inhibitors used to treat rheumatoid and psoriatic arthritis and other auto-immune diseases were sourced from Discovery Life Sciences (Huntsville, AL). SpecimensFull Paper
2021Jöhrens, K;Rüschoff, J;The Challenge to the Pathologist of PD-L1 Expression in Tumor Cells of Non-Small-Cell Lung Cancer-An OverviewCurrent oncology (Toronto, Ont.)34940076Institute of Pathology, Carl Gustav Carus University Hospital Dresden, 01307 Dresden, Germany.++Targos Molecular Pathology GmbH, 34119 Kassel, GermanyFull Paper
2021Hou, X;Wang, G;Fan, W;Chen, X;Mo, C;Wang, Y;Gong, W;Wen, X;Chen, H;He, D;Mo, L;Jiang, S;Ou, M;Guo, H;Liu, H;T-cell receptor repertoires as potential diagnostic markers for patients with COVID-19International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases34688948ad recovered from COVID-19. In the COVID-19-BWNW group, whole blood samples were collected at Bloodworks Northwest (Seattle, WA, USA). In the COVID-19-DLS group, whole blood samples were collected at Discovery Life Sciences (Huntsville, AL, USA). InFull Paper
2021Brosius, C;Caron, K;Sosnoff, C;Blount, B;Wang, L;Rapid Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method to Measure Cannabinoids in Bronchoalveolar-Lavage Fluid of Patients with e-Cigarette, or Vaping, Product Use-Associated Lung InjuryACS OmegaBAL pools were created using anonymous BAL samples acquired commercially from Discovery Life Sciences (Huntsville, AL). They were shipped frozen on dry ice and then stored in −70 C freezers until analyzed. Ten individual BAL fluids were screened foFull Paper
2021Sun, Q;Pastor, L;Du, J;Powell, MJ;Zhang, A;Bodmer, W;Wu, J;Zheng, S;Sha, MY;A novel xenonucleic acid-mediated molecular clamping technology for early colorectal cancer screeningPloS one34610014give an average DNA fragment length of about 150 bp, which mimics the size of cfDNA fragments, justifying their use as cfDNA references. Except for the 10 CRC whole blood samples were purchased from Discovery Life Sciences (Huntsville, AL, US), AllFull Paper
2021Pound, H;Gann, E;Wilhelm, S;A comparative study of metatranscriptomic assessment methods to characterize Microcystis bloomsLimnology and Oceanography: MethodsExtracted RNA was processed using a Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus and then 50-million 100-bp paired-end reads were generated on the Illumina NovaSeq platform at Hudson Alpha Discovery Life Sciences (Huntsville, SaFull Paper
2021Miao, LY;Kim, HJ;Whitlatch, K;Jaiswal, D;Navarro, A;Egan, R;Olivo, PD;A rapid homogenous bioassay for detection of thyroid-stimulating antibodies based on a luminescent cyclic AMP biosensorJournal of immunological methods34871593TSI-positive serum samples were a kind gift of Dr. George Kahaly. Anti-thyroid peroxidase (TPO) antibody-positive human serum samples and nonthyroidal autoimmune disease state human serum samples were obtained from Discovery Life Science (Los Osos, CFull Paper
2021Nuovo, G;Suster, D;Tili, E;Awad, H;Magro, C;A Standardization Protocol for the In Situ Detection of SARS-CoV2 RNA and ProteinsApplied Immunohistochemistry & Molecular Morphologyvid MD‡; Tili, Esmerina PhD§; Awad, Hamdy MD§; Magro, Cynthia MD∥ Author Information __ *Ohio State University Comprehensive Cancer Center §Ohio State University Medical Center, Columbus †Discovery Life Sciences, Powell, OH ‡Department of PatholoFull Paper
2021Nguyen, M;Gary, J;Sonoda, J;Low, M;Ring, C;Bishop, J;Feasibility of Lateral Flow Assays for Urinary Biomarkers to Nicotine Delivery ProductsOSF PREPRINTS2021A prospective collection of 212 individual urine specimens from users and non-nicotine users was completed between July 2020 and April 2021 by Discovery Life Sciences (Los Osos, CA). All enrollees selfidentified as non-users (112 samples) or singleFull Paper
2021Zhang, L;Pohl, C;Homan, K;Sun, V;Gaudette, S;Nelson, C;Erickson, J;Knight, H;Bose, S;Lynch, G;McRae, B;Sterman, A;Mitra, S;Imaging the Alternatively Spliced D Domain of Tenascin C in Preclinical Models of Inflammatory Bowel DiseaseResearch SquareClinical IBD and diseased cynomolgus macaque colon were evaluated using a biotinylated anti-TNC D antibody. Frozen human tissue was obtained from CHTN (National Cancer Institute, Bethesda, MD), Folio Biosciences (Columbus, OH), and UMASS Memorial GasFull Paper
2021Kehl, F;Cretu, V;Willis, P;Open-source lab hardware: Driver and temperature controller for high compliance voltage, fiber-coupled butterfly lasersHardwareXWe recommend applying 12 VDC at the voltage input (VIN), eg, by connecting a wall adapter with sufficient power such as the Qualtek QFWB-65-12-US01, which can provide up to 5 A at 12 VDC. The input voltage is directly fed into a voltage regulator (Full Paper
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2021Hair, J;Robinson, MJ;Wilkinson, RW;Dovedi, SJ;Deep phenotyping of surface stimulatory and inhibitory co-receptors on cancer-resident T and NK cells reveals cell subsets within the tumor-reactive CTL population that are uniquely defined by NKG2A expressionSLAS DiscoveryDisaggregated tumor cells (DTC; Discovery Life Sciences), which are a single-cell suspension of enzymatically-dissociated tumor tissue, from NSCLC (n=13), RC (n=10) and melanoma (n=10) patients were used. Detailed per-patient disease classificationFull Paper
2021Edmund, M;RISING STARSQuality ProgressQuality associate, lead auditor, Discovery Life Sciences (DLS), Powell, OHFull Paper
2021Valle, J;Kelley, R;Nervi, B;Oh, D;Zhu, A;Biliary tract cancerThe LancetIn KEYNOTE-028 PD-L1 positivity was defined as membranous PD-L1 expression in at least 1% of tumour and associated inflammatory cells, or positive staining in stroma (immunohistochemistry assay, QualTek, Goleta, CA, USA), and all patients were PD-L1Full Paper
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2021Skowronska, M;Tiu, C;Tzankov, A;König, F;Lewis, J;Vivanco, I;Kleinschmidt, M;Beebe, K;Anderson, S;Bachmann, F;Engelhardt, M;Lane, H;Kaindl, T;Stan, A;Plummer, E;Evans, T;Zlobec, I;Lopez, J;Expression of end-binding protein 1 (EB1), a potential response-predictive biomarker for lisavanbulin, in glioblastoma and various other solid tumor types.Journal of Clinical Oncologyfor articles by this author. Show More Institute of Pathology, University of Bern, Bern, Switzerland; The Royal Marsden NHS Foundation Trust, Sutton, United Kingdom; Institute of Pathology, University Hospital Basel, Basel, Switzerland; Targos MolecuFull Paper
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2021Fowler, TW;Mitchell, TL;Janda, CY;Xie, L;Tu, S;Chen, H;Zhang, H;Ye, J;Ouyang, B;Yuan, TZ;Lee, SJ;Newman, M;Tripuraneni, N;Rego, ES;Mutha, D;Dilip, A;Vuppalapaty, M;Baribault, H;Yeh, WC;Li, Y;Development of selective bispecific Wnt mimetics for bone loss and repairNature communications34059688The following method was used for human and mouse femur bone (Fig. 1d, e). Fresh human cadaveric femur tissue was purchased from vendor (Folio Conversant). Femur heads were shipped on wet ice within 24 h of death and tissue used immediately upon receFull Paper
2021Butler, D;Mozsary, C;Meydan, C;Foox, J;Rosiene, J;Shaiber, A;Danko, D;Afshinnekoo, E;MacKay, M;Sedlazeck, FJ;Ivanov, NA;Sierra, M;Pohle, D;Zietz, M;Gisladottir, U;Ramlall, V;Sholle, ET;Schenck, EJ;Westover, CD;Hassan, C;Ryon, K;Young, B;Bhattacharya, C;Ng, DL;Granados, AC;Santos, YA;Servellita, V;Federman, S;Ruggiero, P;Fungtammasan, A;Chin, CS;Pearson, NM;Langhorst, BW;Tanner, NA;Kim, Y;Reeves, JW;Hether, TD;Warren, SE;Bailey, M;Gawrys, J;Meleshko, D;Xu, D;Couto-Rodriguez, M;Nagy-Szakal, D;Barrows, J;Wells, H;O'Hara, NB;Rosenfeld, JA;Chen, Y;Steel, PAD;Shemesh, AJ;Xiang, J;Thierry-Mieg, J;Thierry-Mieg, D;Iftner, A;Bezdan, D;Sanchez, E;Campion, TR;Sipley, J;Cong, L;Craney, A;Velu, P;Melnick, AM;Shapira, S;Hajirasouliha, I;Borczuk, A;Iftner, T;Salvatore, M;Loda, M;Westblade, LF;Cushing, M;Wu, S;Levy, S;Chiu, C;Schwartz, RE;Tatonetti, N;Rennert, H;Imielinski, M;Mason, CE;Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactionsNature communications33712587Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.++Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.++Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, UFull Paper
2021Li, J;Conrad, C;Mills, TW;Berg, NK;Kim, B;Ruan, W;Lee, JW;Zhang, X;Yuan, X;Eltzschig, HK;PMN-derived netrin-1 attenuates cardiac ischemia-reperfusion injury via myeloid ADORA2B signalingThe Journal of experimental medicine33891683Materials and methods. MI patient and healthy donor samples. Serum/plasma samples of patients with MI or healthy donors were purchased from Discovery Life Sciences. Corresponding cTnI levels of patients with MI were documented in the patient sample dFull Paper
2021Dang, K;Castello, G;Clarke, SC;Li, Y;Balasubramani, A;Boudreau, A;Davison, L;Harris, KE;Pham, D;Sankaran, P;Ugamraj, HS;Deng, R;Kwek, S;Starzinski, A;Iyer, S;van Schooten, W;Schellenberger, U;Sun, W;Trinklein, ND;Buelow, R;Buelow, B;Fong, L;Dalvi, P;Attenuating CD3 affinity in a PSMAxCD3 bispecific antibody enables killing of prostate tumor cells with reduced cytokine releaseJournal for immunotherapy of cancer34088740Tumor cells were either isolated from fresh prostate tumor tissue procured from LF's lab at UCSF (San Francisco, California, USA) or received as frozen dissociated aliquots along with donor matched PBMCs from Discovery Life Sciences (Newtown, PennsylFull Paper
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2021Saura, C;Matito, J;Oliveira, M;Wildiers, H;Brufksy, AM;Waters, SH;Hurvitz, SA;Moy, B;Kim, SB;Gradishar, WJ;Queiroz, GS;Cronemberger, E;Wallweber, GJ;Bebchuk, J;Keyvanjah, K;Lalani, AS;Bryce, R;Vivancos, A;Eli, LD;Delaloge, S;Biomarker Analysis of the Phase III NALA Study of Neratinib + Capecitabine versus Lapatinib + Capecitabine in Patients with Previously Treated Metastatic Breast CancerClinical cancer research : an official journal of the American Association for Cancer Research34380637Yan (Puma Biotechnology). We thank Jeff Sperinde and Weidong Huang (Monogram Biosciences) for additional support for HERmark and p95 assay design and execution. We thank Sandra Bernard (Targos GMBH) for oversight of clinical sample management. WeFull Paper
2021Pedersen, KS;Foster, NR;Overman, MJ;Boland, PM;Kim, SS;Arrambide, KA;Jaszewski, BL;Bekaii-Saab, T;Graham, RP;Welch, J;Wilson, RH;McWilliams, RR;ZEBRA: A Multicenter Phase II Study of Pembrolizumab in Patients with Advanced Small-Bowel AdenocarcinomaClinical cancer research : an official journal of the American Association for Cancer Research33883178(clinicaltrials.gov). Biomarkers PD-L1 expression was centrally assessed (QualTek, Santa Barbara, CA) by immunohistochemical assays utilizing the Merck 22C3 antibody (Agilent/Dako). Samples required > 50 viable neoplastic cells. Modified Proportion SFull Paper
2021Larson, MH;Pan, W;Kim, HJ;Mauntz, RE;Stuart, SM;Pimentel, M;Zhou, Y;Knudsgaard, P;Demas, V;Aravanis, AM;Jamshidi, A;A comprehensive characterization of the cell-free transcriptome reveals tissue- and subtype-specific biomarkers for cancer detectionNature communications33883548We set out to validate the 23 cell-free mRNA biomarkers identified in our CCGA cohort in an orthogonal set of breast (n = 38) and lung (n = 18) cancer plasma samples obtained from a commercial vendor (Discovery Life Sciences) ;t to validate theFull Paper
2021Lorés-Motta, L;van Beek, AE;Willems, E;Zandstra, J;van Mierlo, G;Einhaus, A;Mary, JL;Stucki, C;Bakker, B;Hoyng, CB;Fauser, S;Clark, SJ;de Jonge, MI;Nogoceke, E;Koertvely, E;Jongerius, I;Kuijpers, TW;den Hollander, AI;Common haplotypes at the CFH locus and low-frequency variants in CFHR2 and CFHR5 associate with systemic FHR concentrations and age-related macular degenerationAmerican journal of human genetics34260947associated with AMD (locus-wide conditioning).15 All the association analyses were performed with the “glm” R function.40 Immunocytochemistry of human retinas Human donor eyes were obtained from Discovery Life Sciences. Eyes were collected within 12Full Paper
2021Egelston, C;Guo, W;Yost, S;Lee, JS;Rose, D;Avalos, C;Ye, J;Frankel, P;Schmolze, D;Waisman, J;Lee, P;Yuan, Y;Pre-existing effector T-cell levels and augmented myeloid cell composition denote response to CDK4/6 inhibitor palbociclib and pembrolizumab in hormone receptor-positive metastatic breast cancerJournal for immunotherapy of cancer33757987PD-L1 stain was performed using 22C3 antibody by Qualtek Molecular LaboratoryFull Paper
2021Campbell, JR;McDonald, BR;Mesko, PB;Siemers, NO;Singh, PB;Selby, M;Sproul, TW;Korman, AJ;Vlach, LM;Houser, J;Sambanthamoorthy, S;Lu, K;Hatcher, SV;Lohre, J;Jain, R;Lan, RY;Fc-optimized Anti-CCR8 Antibody Depletes Regulatory T Cells in Human Tumor ModelsCancer research33757978Study approval Human tumor, skin, spleen, patient blood, and healthy blood leukopack samples were collected through commercial providers (BioIVT, MT Group, Avaden, BioOptions, Discovery Life Sciences, AllCells) or the Cooperative Human Tissue NetworkFull Paper
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2021Yuan, Y;Lee, J;Yost, S;Frankel, P;Ruel, C;Egelston, C;Guo, W;Padam, S;Tang, A;Martinez, N;Schmolze, D;Presant, C;Ebrahimi, B;Yeon, C;Sedrak, M;Patel, N;Portnow, J;Lee, P;Mortimer, J;Phase I/II trial of palbociclib, pembrolizumab and letrozole in patients with hormone receptor-positive metastatic breast cancerEuropean Journal of Cancer2.4. Tumour immune biomarkers Percentage of stromal TILs in tumour was evaluated using haematoxylin and eosin diagnostic sections per International TIL Work Group guideline [23]. PD-L1 was determined by immunohistochemistry (IHC) using the PD-L1 IHCFull Paper
2021Liang, X;Wang, Y;Zhang, Y;Li, B;Radosevich, M;Bacteriophage-host depth distribution patterns in soil are maintained after nutrient stimulation in vitroThe Science of the total environment33991924The extracted DNA samples were quantified using PicoGreen Assay Kit (Invitrogen, Carlsbad, CA) and were then sent to the HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA) for preparation of 16S rRNA gene amplicon library and high-throughpFull Paper
2021Quaas, A;Pamuk, A;Klein, S;Quantius, J;Rehkaemper, J;Barutcu, AG;Rueschoff, J;Zander, T;Gebauer, F;Hillmer, A;Buettner, R;Schroeder, W;Bruns, CJ;Löser, H;Schoemig-Markiefka, B;Alakus, H;Sex-specific prognostic effect of CD66b-positive tumor-infiltrating neutrophils (TANs) in gastric and esophageal adenocarcinomaGastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association34009535Thomas Zander. Institute of Pathology, Nordhessen and Targos Molecular Pathology GmbH, Kassel, Germany. Josef Rueschoff. Authors: Alexander Quaas View author publications. You can also search for this author in PubMed Google ScholarFull Paper
2021Schoemig-Markiefka, B;Eschbach, J;Scheel, AH;Pamuk, A;Rueschoff, J;Zander, T;Buettner, R;Schroeder, W;Bruns, CJ;Loeser, H;Alakus, H;Quaas, A;Optimized PD-L1 scoring of gastric cancerGastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association33954872Thomas Zander. Institute of Pathology Nordhessen, Kassel, Germany. Josef Rueschoff. Targos Molecular Pathology GmbH, Kassel, Germany. Josef Rueschoff & Reinhard Buettner. Authors: Birgid Schoemig-Markiefka View author publicationsFull Paper
2021Onogi, S;Lee, SH;Fruehauf, KR;Shea, KJ;Abiotic Stimuli-Responsive Protein Affinity Reagent for IgGBiomacromolecules34009976Transferrin (80 kDa) from human plasma was obtained from Calbiochem. Human serum was procured from Discovery Life Sciences, Inc. F(ab′) 2 (110 kDa) and Fc (50 kDa) fragments from human IgG were sourced from Jackson ImmunoResearch Laboratories, IncFull Paper
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2021Narla, RK;Modi, H;Bauer, D;Abbasian, M;Leisten, J;Piccotti, JR;Kopytek, S;Eckelman, BP;Deveraux, Q;Timmer, J;Zhu, D;Wong, L;Escoubet, L;Raymon, HK;Hariharan, K;Modulation of CD47-SIRPα innate immune checkpoint axis with Fc-function detuned anti-CD47 therapeutic antibodyCancer immunology, immunotherapy : CII34247273lenalidomide [30]. Cell samples from AML patients were obtained from Conversant Bio (Huntsville) and are described in Supplemental Table 1. CC-90002 binding on cells and blockade of SIRPα to CD47 positive cells. Evaluation ofFull Paper
2021Vos, H;Lambein, K;Richard, F;Mariën, B;Nevelsteen, I;Punie, K;Wildiers, H;Berben, L;Laenen, A;Floris, G;Desmedt, C;Smeets, A;Comparison of the tumor immune microenvironment of primary hormone receptor-negative HER2-positive and triple negative breast cancerNPJ breast cancer34556657PD-L1 (22C3 antibody, 2 μg/mL, Dako-Agilent) immunohistochemistry was performed by Qualtek Molecular Laboratories (Newtown PA, USA) as per agreement with Merck. QualTek provided a modified proportion score (MPSFull Paper
2021Pierson, SK;Shenoy, S;Oromendia, AB;Gorzewski, AM;Langan Pai, RA;Nabel, CS;Ruth, JR;Parente, SAT;Arenas, DJ;Guilfoyle, M;Reddy, M;Weinblatt, M;Shadick, N;Bower, M;Pria, AD;Masaki, Y;Katz, L;Mezey, J;Beineke, P;Lee, D;Tendler, C;Kambayashi, T;Fosså, A;van Rhee, F;Fajgenbaum, DC;Discovery and validation of a novel subgroup and therapeutic target in idiopathic multicentric Castleman diseaseBlood advances34438448this study. We wish to thank Daniel Martinez and the other members of the CHOP Pathology Core as well as Qualtek for their immunohistochemical analyses. We wish to thank Kevin Silk, Giovanni Insana, and James A. Lebovitz for their contributions to thFull Paper
2021Wang, A;Song, Z;Zheng, G;Nicolazzi, C;Fromm, JR;Shehu, E;Srinivasan, S;Chen, X;Zhu, C;Blondel, MC;Adrian, FJ;Evaluation of Preclinical Activity of Isatuximab in Patients with Acute Lymphoblastic LeukemiaMolecular cancer therapeutics34376579centrifugation in line with the manufacturer's instructions. Patient ALL PBMCs were obtained from Conversant Biologics, Inc. Antibodies and reagents Isatuximab was generated in-house. Human immunoglobulin (Ig) G1, IgG4, ATRAFull Paper
2021Reardon, DA;Kim, TM;Frenel, JS;Simonelli, M;Lopez, J;Subramaniam, DS;Siu, LL;Wang, H;Krishnan, S;Stein, K;Massard, C;Treatment with pembrolizumab in programmed death ligand 1-positive recurrent glioblastoma: Results from the multicohort phase 1 KEYNOTE-028 trialCancer33496357QualTek Molecular Laboratories evaluated archived or newly obtained formalin-fixed, paraffin-embedded tumor samples using the 22C3 PD-L1 antibody clone (Merck & Co., Inc., Kenilworth, NJ, USA) and a laboratory- developed prototype immunohistochemicalFull Paper
2021Preillon, J;Cuende, J;Rabolli, V;Garnero, L;Mercier, M;Wald, N;Pappalardo, A;Denies, S;Jamart, D;Michaux, AC;Pirson, R;Pitard, V;Bagot, M;Prasad, S;Houthuys, E;Brouwer, M;Marillier, R;Lambolez, F;Marchante, JR;Nyawouame, F;Carter, MJ;Baron-Bodo, V;Marie-Cardine, A;Cragg, M;Déchanet-Merville, J;Driessens, G;Hoofd, C;Restoration of T-cell Effector Function, Depletion of Tregs, and Direct Killing of Tumor Cells: The Multiple Mechanisms of Action of a-TIGIT Antagonist AntibodiesMolecular cancer therapeutics33277440Cryopreserved isolated PBMCs from healthy volunteers and patients with cancer were provided by ImmunXperts and ConversantBio, respectively. PBMCs were thawed, resuspended in RPMI medium supplemented with 10% FBS + 50 U/mL penicillin/streptomycin (WesFull Paper
2021MacFarlane, AW;Yeung, HM;Alpaugh, RK;Dulaimi, E;Engstrom, PF;Dasari, A;Campbell, KS;Vijayvergia, N;Impacts of pembrolizumab therapy on immune phenotype in patients with high-grade neuroendocrine neoplasmsCancer immunology, immunotherapy : CII33398390PD-L1 immunohistochemistry (IHC) assay was performed at Qualtek Research Laboratories on formalin-fixed, paraffin-embedded tissue sections using anti-PD-L1 monoclonal antibody clone 22C3 (Merck Research Laboratories)Full Paper
2021Holder, C;Adams, A;Allison, C;Cote, O;Lippens, R;Blount, BC;Wang, L;A Novel UHPLC-MS/MS Method for Measuring 8-iso-Prostaglandin F2α in Bronchoalveolar Lavage FluidFrontiers in chemistry34476231Non-EVALI BAL fluids used in this study were acquired from Discovery Life Sciences (Huntsville, AL, United States)Full Paper
2021Rodrigues, D;de Souza, T;Coyle, L;Di Piazza, M;Herpers, B;Ferreira, S;Zhang, M;Vappiani, J;Sévin, DC;Gabor, A;Lynch, A;Chung, SW;Saez-Rodriguez, J;Jennen, DGJ;Kleinjans, JCS;de Kok, TM;New insights into the mechanisms underlying 5-fluorouracil-induced intestinal toxicity based on transcriptomic and metabolomic responses in human intestinal organoidsArchives of toxicology341514005-Fluorouracil (5-FU) is a widely used chemotherapeutical that induces acute toxicity in the small and large intestine of patients. Symptoms can be severe. ;- 5-Fluorouracil (5-FU) is a widely used chemotherapeutical that induces acute toxicity inFull Paper
2021Comunale, BA;Engineer, L;Jiang, Y;Andrews, JC;Liu, Q;Ji, L;Yurkovich, JT;Comunale, RA;Xie, Q;Poliovirus Vaccination Induces a Humoral Immune Response That Cross Reacts With SARS-CoV-2Frontiers in medicine34414206ccination protocols, set by the Centers for Disease Control and Prevention (CDC). IRB approval for the collection of adult sera was obtained from BioMed IRB, and pediatric samples were purchased from Discovery Life Sciences (Huntsville, AL) in accordFull Paper
2021Haynes, WA;Kamath, K;Waitz, R;Daugherty, PS;Shon, JC;Protein-Based Immunome Wide Association Studies (PIWAS) for the Discovery of Significant Disease-Associated AntigensFrontiers in immunology33986742years, with a range of 22-72. Anti-Smith Cohort Samples from 34 subjects that tested positive for Anti-SM RNP (4) or Anti-Smith (30) antibodies by predicate ANA multiplex testing were obtained from Discovery Life Sciences. Subjects ranged in age froFull Paper
2021Kerr, KM;Bibeau, F;Thunnissen, E;Botling, J;Ryška, A;Wolf, J;Öhrling, K;Burdon, P;Malapelle, U;Büttner, R;The evolving landscape of biomarker testing for non-small cell lung cancer in EuropeLung cancer (Amsterdam, Netherlands)33690091He is a co-owner and CSO of Targos Molecular Pathology, Inc., GermanyFull Paper
2021Gentzel, RC;Toolan, D;Jinn, S;Schachter, JB;Ma, L;Kahle, PJ;Smith, SM;Marcus, JN;Intracranial administration of alpha-synuclein fibrils in A30P-synuclein transgenic mice causes robust synucleinopathy and microglial inductionNeurobiology of aging34225000comparison. Neuropathologically confirmed human post-mortem PD brain tissue, acquired from Folio/Discovery Life Sciences (California, USA), or A30P HET brain tissues were serially extracted to obtain a sarkosyl insoluble fractionFull Paper
2021Malik-Chaudhry, HK;Prabhakar, K;Ugamraj, HS;Boudreau, AA;Buelow, B;Dang, K;Davison, LM;Harris, KE;Jorgensen, B;Ogana, H;Pham, D;Schellenberger, U;Van Schooten, W;Buelow, R;Iyer, S;Trinklein, ND;Rangaswamy, US;TNB-486 induces potent tumor cell cytotoxicity coupled with low cytokine release in preclinical models of B-NHLmAbs33818299ground value. The percent of specific lysis was calculated as described above. Ex vivo cytotoxicity assay The ability of TNB-486 to kill CD19+ tumor cells from CLL or DLBCL patient-derived samples (Discovery Life Sciences and IQ Biosciences) was detFull Paper
2021Yamada, M;Eguchi, A;Okuno, K;Sakaguchi, K;Yamaguchi, T;Development of a highly sensitive chemiluminescent enzyme immunoassay for fragmented cytokeratin 18 using new antibodiesScientific reports34521905All methods were carried out in accordance with relevant guidelines and regulations. Human samples. Serum from healthy individuals and NASH patients was purchased from Discovery Life Sciences (CA, USA) with approval and informed consent;defined belowFull Paper
2021Awad, H;Tili, E;Nuovo, G;Kelani, H;Ramadan, ME;Williams, J;Binzel, K;Rajan, J;Mast, D;Efanov, AA;Rasul, KB;Moore, S;Basso, M;Mikhail, A;Eltobgy, M;Malbrue, RA;Bourekas, E;Oglesbee, M;Bergdall, V;Knopp, M;Michaille, JJ;El-Sayed, H;Endovascular repair and open repair surgery of thoraco-abdominal aortic aneurysms cause drastically different types of spinal cord injuryScientific reports33837260The remainder of the tissue was stored at − 80 °C for metabolome analyses. All sections were subsequently analyzed blindly. Immunohistochemistry (IHC). All sections were dealt with blindly using an automated Leica Bond Max platform at Phylogeny Inc.Full Paper
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2021Ermann, J;Matmusaev, M;Haley, E;Braun, C;Jost, F;Mayer-Wrangowski, S;Hsiao, P;Ting, N;Li, L;Terenzio, D;Chime, J;Lukas, S;Patnaude, L;Panzenbeck, M;Csordas, D;Zheng, J;Mierz, D;Simpson, T;King, FJ;Klimowicz, A;Mbow, ML;Fine, JS;Miller, C;Fogal, S;Byrne, FR;The potent and selective RIPK2 inhibitor BI 706039 improves intestinal inflammation in the TRUC mouse model of Inflammatory Bowel DiseaseAmerican journal of physiology. Gastrointestinal and liver physiology34494462Human IBD Intestinal biopsy Immuno-histo-chemical analysis 231 Tissue blocks from surgical resections for ulcerative colitis (n=6) and Crohn's Disease (n=5) were 232 acquired from Discovery Life Sciences. 4 µm sections were fluorescently stained usinFull Paper
2021Reisenauer, KN;Tao, Y;Das, P;Song, S;Svatek, H;Patel, SD;Mikhail, S;Ingros, A;Sheesley, P;Masi, M;Boari, A;Evidente, A;Kornienko, A;Romo, D;Taube, J;Epithelial-mesenchymal transition sensitizes breast cancer cells to cell death via the fungus-derived sesterterpenoid ophiobolin AScientific reports34017048ke to acknowledge Dr. Michelle Nemec, Director of the Molecular Bioscience Center (Baylor University) for assistance with flow cytometry and Dr. Maria Boccia (Baylor University) and Dr. Gerard Nuovo (Discovery Life Sciences, Powell, OH, USA) for assiFull Paper
2021Caldwell, C;Rottman, JB;Paces, W;Bueche, E;Reitsma, S;Gibb, J;Adisetiyo, V;Haas, MS;Heath, H;Newman, W;Baum, J;Gianani, R;Kagey, MH;Validation of a DKK1 RNAscope chromogenic in situ hybridization assay for gastric and gastroesophageal junction adenocarcinoma tumorsScientific reports33972574chromogen kit (Biocare Medical). RNAscope. G/GEJ FFPE tumor resections were commercially acquired from Avaden Biosciences, Discovery Life Sciences or BioOptions following IRB compliance. RNAscope was performed ;emperature for 30 min, washed, andFull Paper
2021Wong, RL;Yu, EY;Refining Immuno-Oncology Approaches in Metastatic Prostate Cancer: Transcending Current LimitationsCurrent treatment options in oncology33433743028 phase 1b trial of pembrolizumab in PD-L1-positive advanced solid tumors included 23 patients with CRPC and PD-L1 expression ≥ 1% in tumor or stroma cells by a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek MolecularFull Paper
2021Meyer, L;Delgado-Cunningham, K;Lorig-Roach, N;Ford, J;DuBois, RM;Human Astrovirus 1-8 Seroprevalence Evaluation in a United States Adult PopulationViruses340704192.7. Human Plasma Samples. Human plasma samples were obtained from Discovery Life Sciences Supplementary Materials. The following are available online at https://www.mdpi. com/article/10.3390/v13060979/s1, Table S1: Plasma Samples from Discovery LiFull Paper
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2021Magro, C;Crowson, A;Franks, L;Schaffer, P;Whelan, P;Nuovo, G;The histologic and molecular correlates of COVID-19 vaccine-induced changes in the skinClinics in Dermatologyonald O. Perelman Department of Dermatology, New York University Grossman School of Medicine, New York, New York eDepartment of Pediatrics, Geffen School of Medicine at UCLA, Los Angeles, California fDiscovery Life Sciences, Powell, Ohio gThe Ohio StFull Paper
2021Magro, C;Nuovo, G;Mulvey, J;Laurence, J;Harp, J;Crowson, A;The skin as a critical window in unveiling the pathophysiologic principles of COVID-19Clinics in DermatologyJoanna MD e Crowson A. Neil MD f aDepartment of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, New York bThe Ohio State University Comprehensive Cancer Center, Columbus Ohio and Discovery Life Sciences, Powell, Ohio cDepartmentFull Paper
2021Xia, B;Blount, BC;Wang, L;Sensitive Quantification of Nicotine in Bronchoalveolar Lavage Fluid by Acetone Precipitation Combined With Isotope-Dilution Liquid Chromatography-Tandem Mass SpectrometryACS omega34124421We directly injected 1 μL of the residual supernatant onto the LC-MS/MS. BAL fluid pools were created for method validation experiments of the method from 12 anonymous BAL fluids purchased commercially from Discovery Life Sciences (Huntsville, AL)Full Paper
2021Cirillo, PDR;Margiotti, K;Fabiani, M;Barros-Filho, MC;Sparacino, D;Cima, A;Longo, SA;Cupellaro, M;Mesoraca, A;Giorlandino, C;Multi-analytical test based on serum miRNAs and proteins quantification for ovarian cancer early detectionPloS one34352040All serum samples from ovarian cancer patients were obtained through MTA (Material Transfer Agreement) signed consent form from the following centers: Discovery Life Sciences (Huntsville, USA), BioIVT (London, UK), Victorian Cancer Bank (Melbourne, AFull Paper
2021Walsh, CS;Kamrava, M;Rogatko, A;Kim, S;Li, A;Cass, I;Karlan, B;Rimel, BJ;Phase II trial of cisplatin, gemcitabine and pembrolizumab for platinum-resistant ovarian cancerPloS one34081738that had membrane staining at low (1+) intensities or greater. An H-score was calculated ranging from 0–300, reflecting the percentage of cells staining at each of the following intensities: negative (1), low (1+), moderate (2+), high (3+). IHC assayFull Paper
2021Bernstein, DE;Trinh, HN;Schiff, ER;Smith, CI;Mospan, AR;Zink, RC;Fried, MW;Lok, AS;Safety and Effectiveness of Tenofovir Alafenamide in Usual Clinical Practice Confirms Results of Clinical Trials: TARGET-HBVDigestive diseases and sciences34059991ERS: Grant support: Eiger, Galmed, Zydus, Celgene, Beckman, Biokit, Bristol Myers Squibb, Conatus, Celgene, Discovery Life Sciences, Genfit, Gilead, Intercept, Novartis, Novo Nordisk, Orasure Technologies, Ortho Diagnostics, Pfizer, Prometheus Lab, RFull Paper
2021Varlet, P;Bouffet, E;Casanova, M;Giangaspero, F;Antonelli, M;Hargrave, D;Ladenstein, R;Pearson, A;Hawkins, C;König, FB;Rüschoff, J;Schmauch, C;Bühnemann, C;Garin-Chesa, P;Schweifer, N;Uttenreuther-Fischer, M;Gibson, N;Ittrich, C;Krämer, N;Solca, F;Stolze, B;Geoerger, B;Comprehensive analysis of the ErbB receptor family in pediatric nervous system tumors and rhabdomyosarcomaPediatric blood & cancer34546642Institute, Medical University, Vienna, Austria 8 Paediatric Drug Development, Children and Young People's Unit, Royal Marsden Hospital, London, UK 9 Division of Clinical Studies, Institute of Cancer Research, London, UK 10 Targos Molecular PathologyFull Paper
2021Bio, S;Nunes, B;Twists and turns of an oyster's life: effects of different depuration periods on physiological biochemical functions of oystersEnvironmental science and pollution research international33559825Aquaculture activities are often established in the vicinity of highly populated, potentially contaminated areas. Animals cultured at such locations, namely bivalves, are frequently used as test organisms in ecotoxicological testing. In this case, aFull Paper
2021Chen, YC;Figliozzi, RW;Hsia, SV;Pilot Analyses of Interferon Subtype Expression Profiles in Patients with Herpes Zoster or Postherpetic NeuralgiaViral immunology33857386A variety of blood samples containing ICD10 codes of HZ or PHN (Table 1) were purchased from Discovery Life Sciences (800 Hudson Way, Suite 1700, Huntsville, AL 35806) The sera of healthy controls were purchased from Discovery Life Sciences with anFull Paper
2021Kam, K;Kumar, V;Lamport, Z;Kymissis, I;A laboratory course on information display technologies for remote learningJournal of the Society for Information DisplaySurvey response percentage, 55.55%. TABLE 2. Listed components and suppliers Component, Brand. AC/DC wall mount adapter 12 V 12 W, Qualtek. LED matrix 5 × 7, Kingbright. Fan axial 40 × 10mm 12VDC wire, CUI Devices. Switch push, C&KFull Paper
2021Rüschoff, J;Baretton, G;Bläker, H;Dietmaier, W;Dietel, M;Hartmann, A;Horn, LC;Jöhrens, K;Kirchner, T;Knüchel, R;Mayr, D;Merkelbach-Bruse, S;Schildhaus, HU;Schirmacher, P;Tiemann, M;Tiemann, K;Weichert, W;Büttner, R;MSI testing : What's new? What should be considered?Der Pathologe34477921Institute of Pathology, Nordhessen und Targos Molecular Pathology GmbH, Germaniastr. 7, 34119, Kassel, Germany. josef.rueschoff@targos-gmbh.de.++Institute of Pathology, University Hospital Carl Gustav Carus, Fetscherstr. 74, 01307, Dresden, Germany.+Full Paper
2021Rüschoff, J;Baretton, G;Bläker, H;Dietmaier, W;Dietel, M;Hartmann, A;Horn, LC;Jöhrens, K;Kirchner, T;Knüchel, R;Mayr, D;Merkelbach-Bruse, S;Schildhaus, HU;Schirmacher, P;Tiemann, M;Tiemann, K;Weichert, W;Büttner, R;[MSI testing : What is new? What should be considered? German version]Der Pathologe34043067What should be considered? German version]. Josef Ruschoff Institut fur Pathologie Nordhessen, TARGOS Molecular Pathology GmbH, Germaniastr Affiliations. 1 author 1. Institut fur Pathologie Nordhessen, TARGOS Molecular Pathology GmbH, GermaniastrFull Paper
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2021Cromer, MK;Camarena, J;Martin, RM;Lesch, BJ;Vakulskas, CA;Bode, NM;Kurgan, G;Collingwood, MA;Rettig, GR;Behlke, MA;Lemgart, VT;Zhang, Y;Goyal, A;Zhao, F;Ponce, E;Srifa, W;Bak, RO;Uchida, N;Majeti, R;Sheehan, VA;Tisdale, JF;Dever, DP;Porteus, MH;Gene replacement of α-globin with β-globin restores hemoglobin balance in β-thalassemia-derived hematopoietic stem and progenitor cellsNature medicine33737751Human CD34+ HSPCs were cultured as previously described18,24,33,36,46,47. CD34+ HSPCs were sourced from fresh cord blood (generously provided by the Binns Family program for Cord Blood Research); from frozen cord blood and Plerixafor- and/or G-CSF-moFull Paper
2021Gaidt, MM;Morrow, A;Fairgrieve, MR;Karr, JP;Yosef, N;Vance, RE;Self-guarding of MORC3 enables virulence factor-triggered immunityNature34759314(PBMCs) from de-identified donors were obtained from AllCells (Alameda) under donor-informed consent and Alpha IRB approval obtained by AllCells (7000-SOP-045) for the study ' SaveFull Paper
2021Mimitou, EP;Lareau, CA;Chen, KY;Zorzetto-Fernandes, AL;Hao, Y;Takeshima, Y;Luo, W;Huang, TS;Yeung, BZ;Papalexi, E;Thakore, PI;Kibayashi, T;Wing, JB;Hata, M;Satija, R;Nazor, KL;Sakaguchi, S;Ludwig, LS;Sankaran, VG;Regev, A;Smibert, P;Scalable, multimodal profiling of chromatin accessibility, gene expression and protein levels in single cellsNature biotechnology34083792Cryopreserved healthy donor peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BM) were obtained from AllCells (USA) or Cellular Technology Limited (CTL) and processed immediately after thawing. NIH-3T3 and HEK293FT cells were maintainFull Paper
2021Lareau, CA;Ludwig, LS;Muus, C;Gohil, SH;Zhao, T;Chiang, Z;Pelka, K;Verboon, JM;Luo, W;Christian, E;Rosebrock, D;Getz, G;Boland, GM;Chen, F;Buenrostro, JD;Hacohen, N;Wu, CJ;Aryee, MJ;Regev, A;Sankaran, VG;Massively parallel single-cell mitochondrial DNA genotyping and chromatin profilingNature biotechnology32788668Cryopreserved PBMCs from patients with CLL consented on institutional review board approved protocols were obtained from AllCells (Patient 1) or from Adrian Wiestner at the SaveFull Paper
2021Hao, Y;Hao, S;Andersen-Nissen, E;Mauck, WM;Zheng, S;Butler, A;Lee, MJ;Wilk, AJ;Darby, C;Zager, M;Hoffman, P;Stoeckius, M;Papalexi, E;Mimitou, EP;Jain, J;Srivastava, A;Stuart, T;Fleming, LM;Yeung, B;Rogers, AJ;McElrath, JM;Blish, CA;Gottardo, R;Smibert, P;Satija, R;Integrated analysis of multimodal single-cell dataCell34062119stom made clone UCHL1 ------------------------- Biological samples ------------------------- Human PBMC Cape Town HVTN Immunology Lab, South Africa https://www.chil.org.za/ Human PBMC AllCells Lot 3032552 -------------------------Full Paper
2021Okuchi, Y;Reeves, J;Ng, SS;Doro, DH;Junyent, S;Liu, KJ;El Haj, AJ;Habib, SJ;Wnt-modified materials mediate asymmetric stem cell division to direct human osteogenic tissue formation for bone repairNature materials32958876hSSCs were isolated from a commercially available bone marrow aspirate (AllCells). The bone marrow aspirate was plated onto culture flasks precoated with 10 ng ml−1 fibronectin (Sigma-Aldrich, 356000) and the cells were allowed to attach. Surface marFull Paper
2021Wu, SZ;Al-Eryani, G;Roden, DL;Junankar, S;Harvey, K;Andersson, A;Thennavan, A;Wang, C;Torpy, JR;Bartonicek, N;Wang, T;Larsson, L;Kaczorowski, D;Weisenfeld, NI;Uytingco, CR;Chew, JG;Bent, ZW;Chan, CL;Gnanasambandapillai, V;Dutertre, CA;Gluch, L;Hui, MN;Beith, J;Parker, A;Robbins, E;Segara, D;Cooper, C;Mak, C;Chan, B;Warrier, S;Ginhoux, F;Millar, E;Powell, JE;Williams, SR;Liu, XS;O'Toole, S;Lim, E;Lundeberg, J;Perou, CM;Swarbrick, A;A single-cell and spatially resolved atlas of human breast cancersNature genetics34493872Percentage of neoplastic cells in each tumor that are classified as each of the SCSubtypes. Tumor samples are grouped according to their Allcells-Pseudobulk classifications. NL, normal-like. c, Representative images of CK5 (top) and ER (bottom) IHC fFull Paper
2021Ge, Y;Tian, T;Huang, S;Wan, F;Li, J;Li, S;Wang, X;Yang, H;Hong, L;Wu, N;Yuan, E;Luo, Y;Cheng, L;Hu, C;Lei, Y;Shu, H;Feng, X;Jiang, Z;Wu, Y;Chi, Y;Guo, X;Cui, L;Xiao, L;Li, Z;Yang, C;Miao, Z;Chen, L;Li, H;Zeng, H;Zhao, D;Zhu, F;Shen, X;Zeng, J;An integrative drug repositioning framework discovered a potential therapeutic agent targeting COVID-19Signal transduction and targeted therapy33895786e performed at a flow rate of 30 μL/min. Data processing and analyses were performed using BIAevaluation 1.1 software. LPS-induced cytokine production in PBMCs Peripheral blood mononuclear cells (Allcells) were cultured at 37 °C at concentration 5%Full Paper
2021Miller, IC;Zamat, A;Sun, LK;Phuengkham, H;Harris, AM;Gamboa, L;Yang, J;Murad, JP;Priceman, SJ;Kwong, GA;Enhanced intratumoural activity of CAR T cells engineered to produce immunomodulators under photothermal controlNature biomedical engineering34385695Primary human CD3+ cells were obtained from an anonymous donor blood after apheresis (AllCells) and were cryopreserved in 90% FBS and 10% dimethylsulfoxide until subsequent SaveFull Paper
2021Frede, J;Anand, P;Sotudeh, N;Pinto, RA;Nair, MS;Stuart, H;Yee, AJ;Vijaykumar, T;Waldschmidt, JM;Potdar, S;Kloeber, JA;Kokkalis, A;Dimitrova, V;Mann, M;Laubach, JP;Richardson, PG;Anderson, KC;Raje, NS;Knoechel, B;Lohr, JG;Dynamic transcriptional reprogramming leads to immunotherapeutic vulnerabilities in myelomaNature cell biology34675390registration was 68.5 years (range 45-80). Myeloma cells were enriched using the EasySep Human CD138 positive selection kit (STEMCELL Technologies). Bone marrow from normal donors was obtained from AllCells. Single cell sorting Bone marrow cells werFull Paper
2021Duault, C;Kumar, A;Taghi Khani, A;Lee, SJ;Yang, L;Activated natural killer cells predict poor clinical prognosis in high-risk B-and T-cell acute lymphoblastic leukemiablood36107453processed from patients with B/T-ALL and healthy donors according to Institutional Review Board policies. Healthy BMMCs were purchased from AllCells (Alameda, CA). Healthy PBMCs were isolated from buffy coats procured from Stanford Blood Center, CityFull Paper
2021Duault, C;Kumar, A;Taghi Khani, A;Lee, SJ;Yang, L;Huang, M;Hurtz, C;Manning, B;Ghoda, L;McDonald, T;Lacayo, NJ;Sakamoto, KM;Carroll, M;Tasian, SK;Marcucci, G;Yu, J;Caligiuri, MA;Maecker, HT;Swaminathan, S;Activated natural killer cells predict poor clinical prognosis in high-risk B- and T-cell acute lymphoblastic leukemiaBlood34077953ripheral blood mononuclear cells (PBMCs) were collected and processed from patients with B/T-ALL and healthy donors according to Institutional Review Board policies. Healthy BMMCs were purchased from AllCells (Alameda, CA). Healthy PBMCs were isolateFull Paper
2021Alavi, A;Krishnamurti, L;Abedi, M;Galeon, I;Reiner, D;Smith, S;Wang, L;Ramezi, A;Rendo, P;Walters, M;Preliminary Safety and Efficacy Results from Precizn-1: An Ongoing Phase 1/2 Study on Zinc Finger Nuclease-Modified Autologous CD34+ HSPCs for Sickle Cell Disease (SCD)BloodIntroduction Sickle cell disease (SCD) is caused by pathologic variants in both alleles of the β-globin gene, affecting ~100,000 patients in the US (Strouse. Handb Clin Neurol 2016;138:311-24). Elevated fetal hemoglobin (HbF) levels amFull Paper
2021Kwiatkowski, J;Locatelli, F;Walters, M;Porter, J;Hongeng, S;Yannaki, E;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Guo, R;Colvin, R;Gruppioni, K;Thompson, A;Improvement in Health-Related Quality of Life Following Treatment with Betibeglogene Autotemcel in Patients with Transfusion-Dependent β-Thalassemia Enrolled in Phase 3 StudiesBloodIntroduction: Patients with transfusion-dependent β-thalassemia (TDT) have increased morbidity and mortality and reduced health-related quality of life (HRQoL) compared with the general population owing to the mental, physical, and time demands of chFull Paper
2021Hall, A;Winestone, L;Sullivan, E;Wu, Q;Lamble, A;Walters, M;Aguayo-Hiraldo, P;Baez Conde, L;Coker, T;Dave, H;Dornsife, D;Keating, A;Merino, D;Ramsey, B;Park, J;Agrawal, A;Access to CAR-T Cell Therapy in Underrepresented Populations: A Multicenter Cohort Study of Pediatric and Young Adult ALL PatientsBloodIntroduction: Social determinants of health such as race, ethnicity, and socioeconomic status (SES) are associated with inferior outcomes in patients with B-cell acute lymphoblastic leukemia (B-ALL). Latinx patients have worse event-free survival andFull Paper
2021Thompson, A;Locatelli, F;Yannaki, E;Walters, M;Porter, J;Hongeng, S;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Cavazzana, M;Rasko, J;Du, L;Colvin, R;Kwiatkowski, J;Restoring Iron Homeostasis in Pts Who Achieved Transfusion Independence after Treatment with Betibeglogene Autotemcel Gene Therapy: Results from up to 7 Years of Follow-upBloodIntroduction: Transfusion-dependent β-thalassemia (TDT) is a severe genetic disease requiring lifelong, regular packed red blood cell (pRBC) transfusions and iron chelation treatment. Betibeglogene autotemcel (beti-cel) is a one-time ex vivo gene theFull Paper
2021Walters, M;Smith, A;Schiller, G;Esrick, E;Williams, D;Gogoleva, T;Rouy, D;Cockroft, B;Vercellotti, G;Updated Results of a Phase 1/2 Clinical Study of Zinc Finger Nuclease-Mediated Editing of BCL11A in Autologous Hematopoietic Stem Cells for Transfusion-Dependent Beta ThalassemiaBloodIntroduction: Persistently elevated fetal hemoglobin (HbF) can ameliorate transfusion-dependent beta thalassemia (TDT) (Musallam et al ., 2012). BCL11A is a repressor of γ-globin expression and HbF production in adult erythrocytes. Down-regulation ofFull Paper
2021Tisdale, J;Thompson, A;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Schmidt, M;Pierciey, F;Whitney, D;Rogers, C;Nnamani, M;Foos, M;Miller, A;Zhang, X;Lynch, J;Walters, M;Kanter, J;Bonner, M;Polyclonality Strongly Correlates with Biological Outcomes and Is Significantly Increased Following Improvements to the Phase 1/2 HGB-206 Protocol and Manufacturing of LentiGlobin for Sickle Cell Disease (SCD; bb1111) Gene Therapy (GT)BloodBackground: The ongoing Phase 1/2 HGB-206 study (NCT02140554) of LentiGlobin for SCD (bb1111) GT uses a modified human β-globin gene that expresses an anti-sickling hemoglobin (HbA T87Q). The relationships between biological outcomes, clinical outcomFull Paper
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2021Kruta, M;Sunshine, MJ;Chua, BA;Fu, Y;Chawla, A;Dillingham, CH;Hidalgo San Jose, L;De Jong, B;Zhou, FJ;Signer, RAJ;Hsf1 promotes hematopoietic stem cell fitness and proteostasis in response to ex vivo culture stress and agingCell stem cell34388375Human cord blood derived CD34 + HSPCs were purchased from AllCells. Cell number and viability were assessed with a hemocytometer based on trypan blue exclusion. SaveFull Paper
2021Turchiano, G;Andrieux, G;Klermund, J;Blattner, G;Pennucci, V;El Gaz, M;Monaco, G;Poddar, S;Mussolino, C;Cornu, TI;Boerries, M;Cathomen, T;Quantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-SeqCell stem cell33626327Experimental models: cell lines human CD34+ HSPCs AllCells Cat.# CB008F human CD34+ HSPCs StemCell-Technologies Cat.# 70008.3 K562 N/A RRID:CVCL_0004; authenticated by STR profilingFull Paper
2021Mondala, PK;Vora, AA;Zhou, T;Lazzari, E;Ladel, L;Luo, X;Kim, Y;Costello, C;MacLeod, AR;Jamieson, CHM;Crews, LA;Selective antisense oligonucleotide inhibition of human IRF4 prevents malignant myeloma regeneration via cell cycle disruptionCell stem cell33476575In multiple myeloma, inflammatory and anti-viral pathways promote disease progression and cancer stem cell generation. Using diverse pre-clinical models, we investigated the role of SaveFull Paper
2021Su, M;Lin, Y;Cui, C;Tian, X;Lai, L;ERMAP is a B7 family-related molecule that negatively regulates T cell and macrophage responsesCellular & molecular immunology32620788T cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan-T labeling system were purchased from ALLCELLS, LLC (Alameda, CA). Murine CD3+ T cells were purified from C57BL/6 mice by an immunomagnetic system (MiltFull Paper
2021Seo, H;Masuda, H;Asagoshi, K;Uchiki, T;Kawata, S;Sasaki, G;Yabuki, T;Miyai, S;Takahashi, N;Hashimoto, SI;Sawada, A;Takaiwa, A;Koyama, C;Tamai, K;Kurosawa, K;Lin, KY;Ohta, K;Nakazaki, Y;Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell lineCellular & molecular immunology32457406The germline-derived divergent hV H harboring CDR3 was prepared from normal human peripheral blood CD19 + B-cell cDNA (ALLCELLS) using degenerate primers that annealed to SaveFull Paper
2021Sadras, T;Martin, M;Kume, K;Robinson, ME;Saravanakumar, S;Lenz, G;Chen, Z;Song, JY;Siddiqi, T;Oksa, L;Knapp, AM;Cutler, J;Cosgun, KN;Klemm, L;Ecker, V;Winchester, J;Ghergus, D;Soulas-Sprauel, P;Kiefer, F;Heisterkamp, N;Pandey, A;Ngo, V;Wang, L;Jumaa, H;Buchner, M;Ruland, J;Chan, WC;Meffre, E;Martin, T;Müschen, M;Developmental partitioning of SYK and ZAP70 prevents autoimmunity and cancerMolecular cell33878293Cord blood derived human CD34 + cells (ALLCELLS) were thawed and were lentivirally transduced with pCL6-ZAP70-GFP or an empty vector control. To obtain optimal transduction, SaveFull Paper
2021Lattanzi, A;Camarena, J;Lahiri, P;Segal, H;Srifa, W;Vakulskas, CA;Frock, RL;Kenrick, J;Lee, C;Talbott, N;Skowronski, J;Cromer, MK;Charlesworth, CT;Bak, RO;Mantri, S;Bao, G;DiGiusto, D;Tisdale, J;Wright, JF;Bhatia, N;Roncarolo, MG;Dever, DP;Porteus, MH;Development of β-globin gene correction in human hematopoietic stem cells as a potential durable treatment for sickle cell diseaseScience translational medicine34135108Human CD34+ HSPCs were cultured in small-scale conditions as previously described (67, 20, 25, 68, 69). Frozen purified CD34+ HSPCs were sourced from plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA and STEMCELL TechnolFull Paper
2021Jain, MS;Polanski, K;Conde, CD;Chen, X;Park, J;Mamanova, L;Knights, A;Botting, RA;Stephenson, E;Haniffa, M;Lamacraft, A;Efremova, M;Teichmann, SA;MultiMAP: dimensionality reduction and integration of multimodal dataGenome biology34930412sed to identify genes whose expression changes significantly along the trajectory. Acquisition and processing of human PBMCs PBMCs from two donors were acquired from a LeukoLab (Clinical division of AllCells). Frozen PBMC samples were thawed quicklyFull Paper
2021Thongon, N;Ma, F;Santoni, A;Marchesini, M;Fiorini, E;Rose, A;Adema, V;Ganan-Gomez, I;Groarke, EM;Gutierrez-Rodrigues, F;Chen, S;Lockyer, P;Schneider, S;Bueso-Ramos, C;Montalban-Bravo, G;Class, CA;Soltysiak, KA;Pellegrini, M;Sahin, E;Bertuch, AA;DiNardo, CD;Garcia-Manero, G;Young, NS;Dwyer, K;Colla, S;Hematopoiesis under telomere attrition at the single-cell resolutionNature communications34824242BM samples from HDs were obtained from AllCells. Written informed consent was obtained from all donors. The clinical characteristics and the PB counts of the individuals with SaveFull Paper
2021Qiao, Y;Qiu, Y;Ding, J;Luo, N;Wang, H;Ling, X;Sun, J;Wu, Z;Wang, Y;Liu, Y;Guo, F;Sun, T;Shen, W;Zhang, M;Wu, D;Chen, B;Xu, W;Wang, X;Cancer immune therapy with PD-1-dependent CD137 co-stimulation provides localized tumour killing without systemic toxicityNature communications34737267Human PBMCs were purchased from Allcells and the informed consent was obtained from all donors. Human PBMCs were cultured in RPMI 1640 medium supplemented with 10% FBS SaveFull Paper
2021Duren, Z;Lu, WS;Arthur, JG;Shah, P;Xin, J;Meschi, F;Li, ML;Nemec, CM;Yin, Y;Wong, WH;Sc-compReg enables the comparison of gene regulatory networks between conditions using single-cell dataNature communications34362918itial analysis has considered the HiChIP data. We will extend our sc-compReg software to enable this type of analysis. Methods Single-cell ATAC Cryopreserved BMMCs from two donors were obtained from AllCells. One donor was a 93-year-old non-HispanicFull Paper
2021Amit, I;Iancu, O;Levy-Jurgenson, A;Kurgan, G;McNeill, MS;Rettig, GR;Allen, D;Breier, D;Ben Haim, N;Wang, Y;Anavy, L;Hendel, A;Yakhini, Z;CRISPECTOR provides accurate estimation of genome editing translocation and off-target activity from comparative NGS dataNature communications34031394ubation at room temperature before the electroporation. Genome editing in mobilized CD34+ HSPCs and in HEK293/HEK293-Cas9 cell lines Human CD34+ HSPCs, derived from mobilized peripheral blood (MPB) (AllCells, Alameda, CA), were thawed and cultured fFull Paper
2021Lal, A;Chiang, ZD;Yakovenko, N;Duarte, FM;Israeli, J;Buenrostro, JD;Deep learning-based enhancement of epigenomics data with AtacWorksNature communications336860690 or 1 representing whether that position was part of a CTCF binding motif on the reverse strand. Generation of dscATAC-seq data for FACS-isolated HSCs Cryopreserved human BMMCs were purchased from Allcells (catalog number BM, CR, MNC, 10 M). CellsFull Paper
2021Sharma, R;Dever, DP;Lee, CM;Azizi, A;Pan, Y;Camarena, J;Köhnke, T;Bao, G;Porteus, MH;Majeti, R;The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cellsNature communications33473139For in vivo studies presented, cord blood-derived CD34 + cells were purchased from AllCells or STEMCELL Technologies and were thawed according to the manufacturer's SaveFull Paper
2021Yang, KD;Belyaeva, A;Venkatachalapathy, S;Damodaran, K;Katcoff, A;Radhakrishnan, A;Shivashankar, GV;Uhler, C;Multi-domain translation between single-cell imaging and sequencing data using autoencodersNature communications33397893nly limited functional data is available by acquiring chromatin imaging data. Methods Cell culture and immunostaning CD4+/CD45RA+ naive helper T-cells from human peripheral blood were purchased from AllCells. These cells were revived and cultured inFull Paper
2021Gnanapragasam, M;Planutis, A;Glassberg, J;Bieker, J;Identification of a genomic DNA sequence that quantitatively modulates KLF1 expression in differentiating human hematopoietic cellsbioRxivCD34+ cells were purchased from AllCells or obtained from the Yale Cooperative Center of Excellence in Hematology. These were differentiated under three-phase protocols, initially based on (Antoniani et al., 2018; Yien and Bieker, 2012) but then lateFull Paper
2021Shy, B;Vykunta, V;Ha, A;Roth, T;Talbot, A;Nguyen, D;Chen, Y;Blaeschke, F;Vedova, S;Mamedov, M;Chung, J;Li, H;Wolf, J;Martin, T;Ye, L;Eyquem, J;Esensten, J;Marson, A;Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturingbioRxivPrimary adult blood cells were obtained from anonymous healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc. or Allcells Inc, or as a Trima residual from Vitalant. If needed, peripheral blood mononuclear cells were iFull Paper
2021Amin, OE;Colbeck, EJ;Daffis, S;Khan, S;Ramakrishnan, D;Pattabiraman, D;Chu, R;Micolochick Steuer, H;Lehar, S;Peiser, L;Palazzo, A;Frey, C;Davies, J;Javanbakht, H;Rosenberg, WMC;Fletcher, SP;Maini, MK;Pallett, LJ;Therapeutic Potential of TLR8 Agonist GS-9688 (Selgantolimod) in Chronic Hepatitis B: Remodeling of Antiviral and Regulatory MediatorsHepatology (Baltimore, Md.)33368377In select experiments, whole blood was obtained from AllCells (Alameda, CA) and the MT Group (Van Nuys, CA). In this instance, consent was obtained from the donor or donor's legal SaveFull Paper
2021Shukla, S;Ying, W;Gray, F;Yao, Y;Simes, ML;Zhao, Q;Miao, H;Cho, HJ;González-Alonso, P;Winkler, A;Lund, G;Purohit, T;Kim, E;Zhang, X;Ray, JM;He, S;Nikolaidis, C;Ndoj, J;Wang, J;Jaremko, Ł;Jaremko, M;Ryan, RJH;Guzman, ML;Grembecka, J;Cierpicki, T;Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domainNature chemical biology34155404CD34-positive bone marrow blood cells were obtained from Allcells (ABM021F) and stored in liquid nitrogen until use. For treatment experiments that lasted more than 4 d, media were SaveFull Paper
2021Jang, S;Song, J;Kim, N;Bak, J;Jung, K;Park, YW;Park, BC;Kim, HM;Development of an antibody-like T-cell engager based on VH-VL heterodimer formation and its application in cancer therapyBiomaterials33774526All healthy donor PBMCs and CD8 + T cells used in this study were purchased from AllCells (#PB004F and #PB009-3 F), Zenbio (#SER-PBMC-200-F), and Lonza (#3W-270). CD3 + T SaveFull Paper
2021Davis-Marcisak, EF;Fitzgerald, AA;Kessler, MD;Danilova, L;Jaffee, EM;Zaidi, N;Weiner, LM;Fertig, EJ;Transfer learning between preclinical models and human tumors identifies a conserved NK cell activation signature in anti-CTLA-4 responsive tumorsGenome medicine34376232Fresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selected from donor peripheral blood using CD56 positivity. Donor NK cell purity SaveFull Paper
2021Steinbuck, MP;Seenappa, LM;Jakubowski, A;McNeil, LK;Haqq, CM;DeMuth, PC;A lymph node-targeted Amphiphile vaccine induces potent cellular and humoral immunity to SARS-CoV-2Science advances33547083scent serum/plasma Convalescent serum samples (n = 7) and plasma samples (n = 15) from patients who had recovered from SARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and AllCells (Alameda, CA), respectively. All samplesFull Paper
2021Lin, Y;Cui, C;Su, M;Silbart, LK;Liu, H;Zhao, J;He, L;Huang, Y;Xu, D;Wei, X;Du, Q;Lai, L;Identification of TAPBPL as a novel negative regulator of T-cell functionEMBO molecular medicine33938620itro T‐cell assays Normal human peripheral blood CD3+ Pan T cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan‐T labeling system were purchased from ALLCELLS, LLC (Alameda, CA). Murine CD3+ T cells were puFull Paper
2021Kim, WT;Hennick, K;Johnson, J;Finnerty, B;Choo, S;Short, SB;Drubin, C;Forster, R;McMaster, ML;Hockemeyer, D;Cancer-associated POT1 mutations lead to telomere elongation without induction of a DNA damage responseThe EMBO journal33934394e aging or age‐associated diseases (Chakravarti et al, 2021). Materials and Methods Cell culture and cryopreservation Human bone marrow CD34+ hematopoietic stem/progenitor cells were obtained from AllCells and Fred Hutchinson Cancer Research Center.Full Paper
2021Deegen, P;Thomas, O;Nolan-Stevaux, O;Li, S;Wahl, J;Bogner, P;Aeffner, F;Friedrich, M;Liao, MZ;Matthes, K;Rau, D;Rattel, B;Raum, T;Kufer, P;Coxon, A;Bailis, JM;The PSMA-targeting Half-life Extended BiTE Therapy AMG 160 has Potent Antitumor Activity in Preclinical Models of Metastatic Castration-resistant Prostate CancerClinical cancer research : an official journal of the American Association for Cancer Research33504551Human T cells were obtained from AllCells. Human peripheral blood mononuclear cells (PBMC) were isolated from buffy coats collected at the Institute of Clinical Transfusion Medicine SaveFull Paper
2021Giffin, MJ;Cooke, K;Lobenhofer, EK;Estrada, J;Zhan, J;Deegen, P;Thomas, M;Murawsky, CM;Werner, J;Liu, S;Lee, F;Homann, O;Friedrich, M;Pearson, JT;Raum, T;Yang, Y;Caenepeel, S;Stevens, J;Beltran, PJ;Canon, J;Coxon, A;Bailis, JM;Hughes, PE;AMG 757, a Half-Life Extended, DLL3-Targeted Bispecific T-Cell Engager, Shows High Potency and Sensitivity in Preclinical Models of Small-Cell Lung CancerClinical cancer research : an official journal of the American Association for Cancer Research33203642human CD3+ T cells (2 × 107) by intravenous infusion on study day 0. Human CD3+ T cells, isolated from PBMCs from two different donors using negative selection, were purchased from AllCells and activated in vitro using IL2 and CD3/CD28/CD2 (STEMCELLFull Paper
2021Kögler, AC;Kherdjemil, Y;Bender, K;Rabinowitz, A;Marco-Ferreres, R;Furlong, EEM;Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryosDevelopmental cell34363757were also taken from regions not overlapping any mCherry-expressing cells (background (BG)) and from regions overlapping the entire group of cells investigated (AllCells) to Save;each time point, the same measurements were also taken from regions nFull Paper
2021Montalban-Bravo, G;Kanagal-Shamanna, R;Darbaniyan, F;Siddiqui, MT;Sasaki, K;Wei, Y;Yang, H;Chien, KS;Naqvi, K;Jabbour, E;Kadia, TM;Daver, N;DiNardo, C;Ravandi, F;Pemmaraju, N;Bose, P;Verstovsek, S;Pierce, S;Bueso-Ramos, C;Patel, K;Do, KA;Kantarjian, H;Garcia-Manero, G;Clinical, genomic, and transcriptomic differences between myelodysplastic syndrome/myeloproliferative neoplasm with ring sideroblasts and thrombocytosis (MDS/MPN-RS-T) and myelodysplastic syndrome with ring sideroblasts (MDS-RS)American journal of hematology33811786seven patients with MDS-RS, and 17 healthy individuals obtained from AllCells (Emeryville, CA) were isolated using the CD34 MicroBead Kit and RNA was isolated using the PicoPure RNA isolation kit, following manufacturer's instructions. Fastq files weFull Paper
2021Kwee, BJ;Lam, J;Akue, A;KuKuruga, MA;Zhang, K;Gu, L;Sung, KE;Functional heterogeneity of IFN-γ-licensed mesenchymal stromal cell immunosuppressive capacity on biomaterialsProceedings of the National Academy of Sciences of the United States of America34446555MSCs obtained from AllCells and Lonza cultured on the two hydrogels (SI Appendix Overall similar observations were obtained with a representative MSC line obtained from AllCells ( SaveFull Paper
2021Cui, L;Moraga, I;Lerbs, T;Van Neste, C;Wilmes, S;Tsutsumi, N;Trotman-Grant, AC;Gakovic, M;Andrews, S;Gotlib, J;Darmanis, S;Enge, M;Quake, S;Hitchcock, IS;Piehler, J;Garcia, KC;Wernig, G;Tuning MPL signaling to influence hematopoietic stem cell differentiation and inhibit essential thrombocythemia progenitorsProceedings of the National Academy of Sciences of the United States of America33384332Healthy deidentified human bone marrow samples were acquired from AllCells. Essential thrombocytosis bone marrow samples were deidentified and acquired from the Stanford SaveFull Paper
2021Poussin, M;Sereno, A;Wu, X;Huang, F;Manro, J;Cao, S;Carpenito, C;Glasebrook, A;Powell, DJ;Demarest, S;Dichotomous impact of affinity on the function of T cell engaging bispecific antibodiesJournal for immunotherapy of cancer34253637w cytometric evaluation of cell surface proteins on T cells 48 hours after mixing SKOV3 cells and biologic test articles is further described in online supplemental methods. Briefly, human T cells (ALLCELLS frozen normal human peripheral blood CD3+ PFull Paper
2021Roselli, E;Boucher, JC;Li, G;Kotani, H;Spitler, K;Reid, K;Cervantes, EV;Bulliard, Y;Tu, N;Lee, SB;Yu, B;Locke, FL;Davila, ML;4-1BB and optimized CD28 co-stimulation enhances function of human mono-specific and bi-specific third-generation CAR T cellsJournal for immunotherapy of cancer34706886Human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells. T cells were enriched from PBMCs using the EasySep human T cell isolation kit according to SaveFull Paper
2021Paavola, KJ;Roda, JM;Lin, VY;Chen, P;O'Hollaren, KP;Ventura, R;Crawley, SC;Li, B;Chen, HH;Malmersjö, S;Sharkov, NA;Horner, G;Guo, W;Kutach, AK;Mondal, K;Zhang, Z;Lichtman, JS;Song, C;Rivera, LB;Liu, W;Luo, J;Wang, Y;Solloway, MJ;Allan, BB;Kekatpure, A;Starck, SR;Haldankar, R;Fan, B;Chu, C;Tang, J;Molgora, M;Colonna, M;Kaplan, DD;Hsu, JY;The Fibronectin-ILT3 Interaction Functions as a Stromal Checkpoint that Suppresses Myeloid CellsCancer immunology research34426457Human peripheral blood mononuclear cells (PBMC) of healthy donors were isolated from freshly drawn leukopaks (Allcells, catalog no. PB007) by density gradient centrifugation over SaveFull Paper
2021Badeaux, MD;Rolig, AS;Agnello, G;Enzler, D;Kasiewicz, MJ;Priddy, L;Wiggins, JF;Muir, A;Sullivan, MR;Van Cleef, J;Daige, C;Vander Heiden, MG;Rajamanickam, V;Wooldridge, JE;Redmond, WL;Rowlinson, SW;Arginase Therapy Combines Effectively with Immune Checkpoint Blockade or Agonist Anti-OX40 Immunotherapy to Control Tumor GrowthCancer immunology research33500272Primary human bone marrow (AllCells) was obtained from healthy patients and cultured as follows. Briefly, Hs5 cells were cultured for 72 hours in SFEM2 media (Stem Cell SaveFull Paper
2021Boucher, JC;Li, G;Kotani, H;Cabral, ML;Morrissey, D;Lee, SB;Spitler, K;Beatty, NJ;Cervantes, EV;Shrestha, B;Yu, B;Kazi, A;Wang, X;Sebti, SM;Davila, ML;CD28 Costimulatory Domain-Targeted Mutations Enhance Chimeric Antigen Receptor T-cell FunctionCancer immunology research33188139An obstacle to the development of chimeric antigen receptor (CAR) T cells is the limited understanding of CAR T-cell biology and the mechanisms behind their antitumor activity. We and SaveFull Paper
2021Li, J;Whelan, S;Kotturi, MF;Meyran, D;D'Souza, C;Hansen, K;Liang, S;Hunter, J;Trapani, JA;Neeson, PJ;PVRIG is a novel natural killer cell immune checkpoint receptor in acute myeloid leukemiaHaematologica33147937nt clinical characteristics are summarised in the Online Supplementary Table S2. Healthy donor bone marrow samples were obtained from Royal Melbourne Hospital (Melbourne, Australia) or purchased from AllCells (Alameda, California). All bone marrow saFull Paper
2021Gilmartin, AG;Groy, A;Gore, ER;Atkins, C;Long, ER;Montoute, MN;Wu, Z;Halsey, W;McNulty, DE;Ennulat, D;Rueda, L;Pappalardi, M;Kruger, RG;McCabe, MT;Raoof, A;Butlin, R;Stowell, A;Cockerill, M;Waddell, I;Ogilvie, D;Luengo, J;Jordan, A;Benowitz, AB;In vitro and in vivo induction of fetal hemoglobin with a reversible and selective DNMT1 inhibitorHaematologica32586904Cryopreserved human bone marrow CD34+ cells (AllCells) were confirmed to be sourced ethically, and their research use was in accord with the terms of the informed consents under SaveFull Paper
2021Zhang, C;Zhang, X;Huang, L;Guan, Y;Huang, X;Tian, XL;Zhang, L;Tao, W;ATF3 drives senescence by reconstructing accessible chromatin profilesAging cell33539668accessibility in IARs. Accordingly, our research provides a target to aim for in the exploration of senescence and aging. 4 EXPERIMENTAL PROCEDURES 4.1 Cell culture We purchased primary HUVECs from AllCells Biotech Shanghai Co., Ltd., and cultured tFull Paper
2021Nesterenko, PA;McLaughlin, J;Tsai, BL;Burton Sojo, G;Cheng, D;Zhao, D;Mao, Z;Bangayan, NJ;Obusan, MB;Su, Y;Ng, RH;Chour, W;Xie, J;Li, YR;Lee, D;Noguchi, M;Carmona, C;Phillips, JW;Kim, JT;Yang, L;Heath, JR;Boutros, PC;Witte, ON;HLA-A∗02:01 restricted T cell receptors against the highly conserved SARS-CoV-2 polymerase cross-react with human coronavirusesCell reports34919800Cryo preserved peripheral blood mononuclear cells (PBMCs) were commercially purchased (Allcells and Hemacare) or obtained from the CFAR Virology Core Laboratory at the UCLA Save;d virus strains ------------------------- NFAT-zsGreen virus UCLA,Full Paper
2021Qian, Y;Arellano, G;Ifergan, I;Lin, J;Snowden, C;Kim, T;Thomas, JJ;Law, C;Guan, T;Balabanov, RD;Kaech, SM;Miller, SD;Choi, J;ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosisCell reports34433042FN-γ, 1 μg/mL anti-IL2 (Cat #554375, clone: S4B6, BD). Isolation of human CD4+ naive T cells and Th cells differentiation Fresh Leukopak samples from de-identified blood donors were purchased from AllCells, LLC. Peripheral blood mononuclear cells (PFull Paper
2021Hamilton, JR;Tsuchida, CA;Nguyen, DN;Shy, BR;McGarrigle, ER;Sandoval Espinoza, CR;Carr, D;Blaeschke, F;Marson, A;Doudna, JA;Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineeringCell reports34077734ons. Isolation and culture of human primary T cells Primary adult blood cells were obtained from anonymous healthy human donors as a leukoreduction pack purchased from StemCell Technologies, Inc. or Allcells Inc, or as a Trima residual from VitalantFull Paper
2021Jiang, Q;Isquith, J;Ladel, L;Mark, A;Holm, F;Mason, C;He, Y;Mondala, P;Oliver, I;Pham, J;Ma, W;Reynoso, E;Ali, S;Morris, IJ;Diep, R;Nasamran, C;Xu, G;Sasik, R;Rosenthal, SB;Birmingham, A;Coso, S;Pineda, G;Crews, L;Donohoe, ME;Venter, JC;Whisenant, T;Mesa, RA;Alexandrov, LB;Fisch, KM;Jamieson, C;Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolutionCell reports33503434Aged normal and MPN Patient samples Obtained through patients consenting at UCSD Moores Cancer Center according to Institutional Review Boardapproved protocols. N/A Young Cord blood CD34+ cells Purchased from AllCells or StemCell TechnologiesFull Paper
2021Bunis, DG;Bronevetsky, Y;Krow-Lucal, E;Bhakta, NR;Kim, CC;Nerella, S;Jones, N;Mendoza, VF;Bryson, YJ;Gern, JE;Rutishauser, RL;Ye, CJ;Sirota, M;McCune, JM;Burt, TD;Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T CellsCell reports33406429Matched adult peripheral blood and BM samples were obtained from healthy volunteer donors through AllCells (Alameda, CA). UCB and longitudinal post-natal samples were obtained SaveFull Paper
2021Yang, Z;Huang, Y;Zhu, L;Yang, K;Liang, K;Tan, J;Yu, B;SIRT6 promotes angiogenesis and hemorrhage of carotid plaque via regulating HIF-1α and reactive oxygen speciesCell death & disease33436551oCl2) was purchased from Sigma-Aldrich, Inc. (Burlington, MA, USA). The work concentration and time were confirmed by instructions combined with experimental requirements. HUVECs were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured inFull Paper
2021Yang, Z;Wei, X;Pan, Y;Xu, J;Si, Y;Min, Z;Yu, B;A new risk factor indicator for papillary thyroid cancer based on immune infiltrationCell death & disease33414407d. The sections were fixed and images were obtained with an inverted microscope (Olympus IX71, Japan). Cell culture and co-culture Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured inFull Paper
2021Everitt, ML;Boegner, DJ;Birukov, KG;White, IM;Sample-to-Answer Diagnostic System for the Detection of Circulating Histones in Whole BloodACS sensors34270219Varying concentrations of histone octamers (Sigma-Aldrich) (0-65 μg/mL final concentrations) are suspended in either fetal bovine serum (FBS) (ATCC) or whole blood (AllCells) (medium is indicated in figure caption). Fifteen microliters of histone-spiFull Paper
2021Varricchio, L;Iancu-Rubin, C;Upadhyaya, B;Zingariello, M;Martelli, F;Verachi, P;Clementelli, C;Denis, JF;Rahman, AH;Tremblay, G;Mascarenhas, J;Mesa, RA;O'Connor-McCourt, M;Migliaccio, AR;Hoffman, R;TGF-β1 protein trap AVID200 beneficially affects hematopoiesis and bone marrow fibrosis in myelofibrosisJCI insight34383713n and response to TGF-β. Methods Further information and complete unedited blots can be found in Supplemental Methods. Human subjects. Primary ND specimens were purchased from a commercial source (AllCells, LLC), whereas MF MNCs were provided by thFull Paper
2021Pan, Y;You, Y;Sun, L;Sui, Q;Liu, L;Yuan, H;Chen, C;Liu, J;Wen, X;Dai, L;Sun, H;The STING antagonist H-151 ameliorates psoriasis via suppression of STING/NF-κB-mediated inflammationBritish journal of pharmacology34460100Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells Shanghai and were used immediately after resuscitation. H-151 was synthesized according to the published SaveFull Paper
2021Hu, X;Qie, C;Jiang, J;Xie, X;Chen, W;Liu, W;Liu, J;M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTABritish journal of pharmacology33450048Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells and were used immediately after resuscitation; these cells cannot be subcultured. Human CD4 + T cells SaveFull Paper
2021Wang, X;Guo, J;Wen, J;Zhang, X;Cao, L;Zeng, D;Liu, X;Jiang, X;Novel vascular strategies on polyetheretherketone modification in promoting osseointegration in ovariectomized ratsMaterials & DesignHUVECs were purchased from AllCells Biology Technology Company (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, Lonza) without endothelial cell growth supplementation. HUVECs at a density of 2 × 104 cells per mL were seeded oFull Paper
2021Iftakhar-E-Khuda, I;Pessia, A;Zheng, S;Kankainen, M;Kontro, M;Karikoski, M;Laurila, J;Gerke, H;Tadayon, S;Hollmén, M;Tang, J;Imhof, BA;Salmi, M;Jalkanen, S;Vascular adhesion protein-1 defines a unique subpopulation of human hematopoietic stem cells and regulates their proliferationCellular and molecular life sciences : CMLS34719737Five hundred cryopreserved and thawed human BM CD34 + cells (AllCells) were cultured in complete methylcellulose-based medium (H4436, STEMCELL Technologies) with or SaveFull Paper
2021Huang, YJ;Cao, J;Lee, CY;Wu, YM;Umbilical cord blood plasma-derived exosomes as a novel therapy to reverse liver fibrosisStem cell research & therapy34772443Human UCB and peripheral blood (PB) plasma were purchased from AllCells LLC (Alameda, CA), and exosomes were acquired using ExoQuick Exosome isolation reagent (System SaveFull Paper
2021Pieles, O;Reichert, TE;Morsczeck, C;Classical isoforms of protein kinase C (PKC) and Akt regulate the osteogenic differentiation of human dental follicle cells via both β-catenin and NF-κBStem cell research & therapy33853677to examine the role of classical PKC isoforms and the potential downstream mechanisms during the osteogenic differentiation of DFCs. Materials and methods Cell culture Human DFCs were purchased from AllCells (Emeryville, USA) and grown in Dulbecco’sFull Paper
2021Lin, Z;Liu, X;Liu, T;Gao, H;Wang, S;Zhu, X;Rong, L;Cheng, J;Cai, Z;Xu, F;Tan, X;Lv, L;Li, Z;Sun, Y;Qian, Q;Evaluation of Nonviral piggyBac and lentiviral Vector in Functions of CD19chimeric Antigen Receptor T Cells and Their Antitumor Activity for CD19+ Tumor CellsFrontiers in immunology35082789Peripheral blood mononuclear cells (PBMCs) from whole blood were purchased from AllCells (Shanghai, China) and isolated by Ficoll density gradient centrifugation (Cat#17-5442-03, SaveFull Paper
2021Cui, X;Jia, H;Xin, H;Zhang, L;Chen, S;Xia, S;Li, X;Xu, W;Chen, X;Feng, Y;Wei, X;Yu, H;Wang, Y;Zhan, Y;Zhu, X;Zhang, X;A Novel Bispecific Antibody Targeting PD-L1 and VEGF With Combined Anti-Tumor ActivitiesFrontiers in immunology34925354Human PBMCs and DCs were acquired by using the Easy MLR Kit (Allcells, Alameda). DCs were treated with mitomycin (H19999025, Hisun Pharm Co., Ltd.) for 30 min before co- SaveFull Paper
2021Ceglia, V;Kelley, EJ;Boyle, AS;Zurawski, S;Mead, HL;Harms, CE;Blanck, JP;Flamar, AL;Kirschman, JH;Ogongo, P;Ernst, JD;Levy, Y;Zurawski, G;Altin, JA;A Framework to Identify Antigen-Expanded T Cell Receptor Clusters Within Complex RepertoiresFrontiers in immunology34917073cificity, are described in other studies (16, 17, 19). Cohesin-Influenza Matrix 1 (Flu M1) protein is described in (16). Donors Cryopreserved human PBMC from normal donors were sourced commercially (AllCells, CA. ND1001 ID:A5983, ND1002 ID:9441, ND1Full Paper
2021Teo Hansen Selnø, A;Schlichtner, S;Yasinska, IM;Sakhnevych, SS;Fiedler, W;Wellbrock, J;Berger, SM;Klenova, E;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV;High Mobility Group Box 1 (HMGB1) Induces Toll-Like Receptor 4-Mediated Production of the Immunosuppressive Protein Galectin-9 in Human Cancer CellsFrontiers in immunology34234778Medical Centre Hamburg-Eppendorf (Ethik Kommission der Ärztekammer Hamburg, reference: PV3469). Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were also purchased from AllCells (Alameda, CA, USA) and handled in accordancFull Paper
2021Xie, X;Chen, C;Chen, W;Jiang, J;Wang, L;Li, T;Sun, H;Liu, J;Structural Basis of VSIG3: The Ligand for VISTAFrontiers in immunology33841409azole buffer (GenScript). Protein was concentrated using 10K MWCO spin columns (Merck). The purified protein was used for functional verification. Cell Culture and Reagents PBMCs were purchased from Allcells Biotechnology (Shanghai) Co., LTD, and weFull Paper
2021Jost, M;Jacobson, AN;Hussmann, JA;Cirolia, G;Fischbach, MA;Weissman, JS;CRISPR-based functional genomics in human dendritic cellseLife33904395t al., 2018 Referred to as ‘B. theta 4PP’ in the text Mutant producing tetra-acylated, di-phosphorylated lipid A in a capsule-producing background Biological sample (human) PBMCs AllCells Freshly isolated from de-identified healtFull Paper
2021Gao, X;Guan, M;Liu, X;Xu, H;Huang, Q;Chen, L;Huang, S;Xiao, Y;Shi, X;Lin, Z;Sustained delivery of growth factors and alendronate using partially demineralized dentin matrix for endogenous periodontal regenerationApplied Materials Todaycells (HUVECs) were purchased from AllCells (Shanghai, China). HUVECs were cultured in endothelial cell growth medium (EGM; AllCells, Shanghai, China). Angiogenesis SaveFull Paper
2021Scherer, SD;Riggio, AI;Haroun, F;DeRose, YS;Ekiz, HA;Fujita, M;Toner, J;Zhao, L;Li, Z;Oesterreich, S;Samatar, AA;Welm, AL;An immune-humanized patient-derived xenograft model of estrogen-independent, hormone receptor positive metastatic breast cancerBreast cancer research : BCR34717714CD34 + hematopoietic stem cells (HSCs) isolated from healthy donor bone marrow (BM) were purchased from AllCells. On the day of injection, cells were thawed in a 37 C water bath SaveFull Paper
2021Nada, MH;Wang, H;Hussein, AJ;Tanaka, Y;Morita, CT;PD-1 checkpoint blockade enhances adoptive immunotherapy by human Vγ2Vδ2 T cells against human prostate cancerOncoimmunology34712512e IgG1 (clone MOPC-21) antibodies were from Bio X Cell (West Lebanon, NH). Ex vivo expansion of Vγ2Vδ2 T cells by pulse zoledronic acid stimulation PBMC were isolated from random donor leukopaks (AllCells, LLC, Alameda, CA) by density centrifugationFull Paper
2021Jiang, H;Ni, H;Zhang, P;Guo, X;Wu, M;Shen, H;Wang, J;Wu, W;Wu, Z;Ding, J;Tang, R;Zhou, S;Chen, B;Yu, M;Jing, H;Liu, J;PD-L1/LAG-3 bispecific antibody enhances tumor-specific immunityOncoimmunology34239776oduced by Innovent Biologics Co., Ltd. (Suzhou, China). Human IgG was purchased from Equitech-Bio (Kerrville). Peripheral blood mononuclear cells (PBMCs) and dendritic cells (DCs) were purchased from AllCells (Shanghai, China). Cynomolgus macaque PBMFull Paper
2021Wang, X;Peticone, C;Kotsopoulou, E;Göttgens, B;Calero-Nieto, FJ;Single-cell transcriptome analysis of CAR T-cell products reveals subpopulations, stimulation, and exhaustion signaturesOncoimmunology33489472or samples PBMCs were derived from healthy donors leukapheresis. Peripheral blood leukapaheresis were obtained from the NHS as part of a research study (IRAS ID 185945) or purchased as LeukoPaks from AllCells. Manufacturing of CAR T cells product GeFull Paper
2021Zhang, C;Delawary, M;Huang, P;Korchak, JA;Suda, K;Zubair, AC;IL-10 mRNA Engineered MSCs Demonstrate Enhanced Anti-Inflammation in an Acute GvHD ModelCells34831324g T7 promoter, 5′ UTRs, 3′ UTRs, and poly-A tail. 2.3. MSC Culture and Time Course Experiment Human MSCs were generated from commercial de-identified bone marrow (Catalog# ABM002) purchased from AllCells (Alameda, CA, USA). The donor was a healthy 2Full Paper
2021Ahn, Y;An, JH;Yang, HJ;Lee, DG;Kim, J;Koh, H;Park, YH;Song, BS;Sim, BW;Lee, HJ;Lee, JH;Kim, SU;Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like SystemCells34440805Human Induced Pluripotent Stem Cells (hiPSCs) and Culture Conditions The hiPSCs were reprogrammed from primary human bone marrow cells (Allcells, Emeryville, CA, USA) as Save;The hiPSCs were reprogrammed from primary human bone marrow cells (AllcelFull Paper
2021Dogan, F;Aljumaily, RMK;Kitchen, M;Forsyth, NR;DNMT3B Is an Oxygen-Sensitive De Novo Methylase in Human Mesenchymal Stem CellsCells33925659nd Methods 2.1. Isolation and Culture of BM-hMSCs Three human BM-hMSCs BMA-16 (Male, Age—29 years, Lonza, MD, USA), BMA-20 (Female, Age—50 years, Lonza, MD, USA) and BMA-25 (Male, Age—27 years, AllCells, LLC, Alameda, CA, USA) were isolated from commFull Paper
2021Miao, G;Sun, X;Development of a novel anti-B7-H4 antibody enhances anti-tumor immune response of human T cellsBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapie34328096Human PBMCs were purchased from ALLCELLS. Hybridoma cell lines were cultured in H-SFM complete medium. CD8+ T cells were cultured in RPMI 1640 medium containing 2 mM L- SaveFull Paper
2021Gu, C;Cao, X;Wang, Z;Hu, X;Yao, Y;Zhou, Y;Liu, P;Liu, X;Gao, G;Hu, X;Zhang, Y;Chen, Z;Gao, L;Peng, Y;Jia, F;Shan, C;Yu, L;Liu, K;Li, N;Guo, W;Jiang, G;Min, J;Zhang, J;Yang, L;Shi, M;Hou, T;Li, Y;Liang, W;Lu, G;Yang, C;Wang, Y;Xia, K;Xiao, Z;Xue, J;Huang, X;Chen, X;Ma, H;Song, D;Pan, Z;Wang, X;Guo, H;Liang, H;Yuan, Z;Guan, W;Deng, SJ;A human antibody of potent efficacy against SARS-CoV-2 in rhesus macaques showed strong blocking activity to B.1.351mAbs34097570ed SARS-CoV-2 RBD (Sino Biological, 40592-V05H). Phage libraries were constructed using antibody gene fragments amplified from peripheral blood mononuclear cells (PBMCs) of 50 healthy human subjects (Allcells, PB003F and PB003 C) and total RNA from PFull Paper
2021Chen, J;Ding, J;Wu, Y;Zhang, S;Zheng, N;Yang, J;Xu, J;Chromium Oxide Nanoparticle Impaired Osteogenesis and Cellular Response to Mechanical StimulusInternational journal of nanomedicine34511912Human MSCs were isolated from bone marrow aspirate (ALLCELLS, Lot No: BM2893). The cells were grown in α-MEM (Gibco, Thermo Fisher Scientific, UK) supplemented with 10% SaveFull Paper
2021Shin, Y;Park, CM;Kim, HG;Kim, DE;Choi, MS;Kim, JA;Choi, BS;Yoon, CH;Identification of Aristolactam Derivatives That Act as Inhibitors of Human Immunodeficiency Virus Type 1 Infection and Replication by Targeting Tat-Mediated Viral TranscriptionVirologica Sinica32779073Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Alameda, CA, USA) and cultured, as described previously (Yoon et al. 2015). HIV-1 clones pNL4-3 and AD8, as well as TZM-bl and A3.01 cells, were obtained from the National InstiFull Paper
2021Wang, Y;Ni, H;Zhou, S;He, K;Gao, Y;Wu, W;Wu, M;Wu, Z;Qiu, X;Zhou, Y;Chen, B;Pan, D;Huang, C;Li, M;Bian, Y;Yang, M;Miao, L;Liu, J;Tumor-selective blockade of CD47 signaling with a CD47/PD-L1 bispecific antibody for enhanced anti-tumor activity and limited toxicityCancer immunology, immunotherapy : CII32761423CD4+ T cells were isolated from human PBMC (AllCells) using EasySep human CD4+ T cells enrichment kit (StemCell Technologies). DCs were generated by incubating PBMC first SaveFull Paper
2021Luban, J;Sattler, RA;Mühlberger, E;Graci, JD;Cao, L;Weetall, M;Trotta, C;Colacino, JM;Bavari, S;Strambio-De-Castillia, C;Suder, EL;Wang, Y;Soloveva, V;Cintron-Lue, K;Naryshkin, NA;Pykett, M;Welch, EM;O'Keefe, K;Kong, R;Goodwin, E;Jacobson, A;Paessler, S;Peltz, SW;The DHODH inhibitor PTC299 arrests SARS-CoV-2 replication and suppresses induction of inflammatory cytokinesVirus research33249060c. Uridine, brequinar, and A77 were obtained from Sigma-Aldrich (St. Louis, MO). Anti IL-4 and anti-IFL-γ antibodies were obtained from BD Biosciences (San Jose, CA). Frozen PBMCs were obtained from AllCells (Alameda, CA). IL-17, IL-17 F and IL-23 ELFull Paper
2021Satani, N;Zhang, X;Giridhar, K;Wewior, N;Cai, C;Aronowski, J;Savitz, SI;A Combination of Atorvastatin and Aspirin Enhances the Pro-Regenerative Interactions of Marrow Stromal Cells and Stroke-Derived Monocytes In VitroFrontiers in pharmacology33959001d of stroke patients. Methods Isolation and Culture of Human Mesenchymal Stromal Cells (MSCs) MSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old male (AllCells, Alameda, CA) using density centrifugationFull Paper
2021Sun, LK;Engineering genetic thermal switches for remote control of therapeutic T cellsThesisPrimary human CD3+ T cells were obtained from anonymous donor blood following apheresis (AllCells) and were cryopreserved until subsequent use. Two days prior to lentiviral SaveFull Paper
2021Wu, HH;Ralph, KL;Sepuldeva, E;Hansen, G;Li, H;Huang, ZF;Liu, D;Dziegelewski, M;Ahlberg, J;Frego, L;Fogal, S;van Tongeren, S;Grimaldi, C;Litzenberger, T;Presky, D;Singh, S;Brodeur, S;Kroe-Barrett, R;An optimally designed anti-human CD40 antibody with potent B cell suppression for the treatment of autoimmune diseasesInternational journal of pharmaceutics34624444Effector cells were generated by stimulating human peripheral blood mononuclear cells (PBMCs) (AllCells) with 10 ng/mL human IL-2 (R&D Systems) in RPMI 1640 medium (Invitrogen) SaveFull Paper
2021Xiang, H;Chan, AG;Ahene, A;Bellovin, DI;Deng, R;Hsu, AW;Jeffry, U;Palencia, S;Powers, J;Zanghi, J;Collins, H;Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancermAbs34719330the full-length human FGFR2b described as Ba/F3 FGFR2b, and the effector cells were peripheral blood mononuclear cells obtained fresh from individual human donors (AllCells; SaveFull Paper
2021Li, G;Guo, J;Zheng, Y;Ding, W;Han, Z;Qin, L;Mo, W;Luo, M;CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T cellsMolecular therapy oncolytics34553036CAR-T cells were generated from commercial normal donor peripheral blood mononuclear cells (PBMCs) from AllCells. The cells were further enriched from PBMC by CD3 magnetic Save;.22-μm filter, aliquoted to 2 mL cryotubes, quick frozen on dry ice, anFull Paper
2021Chen, X;Yang, S;Li, S;Qu, Y;Wang, HY;Liu, J;Dunn, ZS;Cinay, GE;MacMullan, MA;Hu, F;Zhang, X;Wang, P;Secretion of bispecific protein of anti-PD-1 fused with TGF-β trap enhances antitumor efficacy of CAR-T cell therapyMolecular therapy oncolytics33981830Frozen human PBMCs were obtained from AllCells. The T cell culture medium (TCM) was composed of AIM-V medium with 5% (v/v) human AB serum, 10 mM HEPES, 1% (v/v) Save;ng gag-pol. The supernatants were harvested at 48 h after transfection and filtereFull Paper
2021Branquinho, MV;Ferreira, SO;Alvites, RD;Magueta, AF;Ivanov, M;Sousa, AC;Amorim, I;Faria, F;Fernandes, MHV;Vilarinho, PM;Maurício, AC;In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering-A Preliminary StudyInternational journal of molecular sciences34299274e enthalpy of fusion of 100% crystalline material [54]. (1)XC (%)=ΔHfΔHf0×100 4.3. In Vitro Assays 4.3.1. Cell Culture and Maintenance Human Dental Pulp stem/stromal cells (hDPSCs) obtained from AllCells, LLC, Alameda, CA, USA (Cat. DP0037F, Lot no.Full Paper
2021Thavapalachandran, S;Le, TYL;Romanazzo, S;Rashid, FN;Ogawa, M;Kilian, KA;Brown, P;Pouliopoulos, J;Barry, AM;Fahmy, P;Kelly, K;Kizana, E;Chong, JJH;Pluripotent stem cell-derived mesenchymal stromal cells improve cardiac function and vascularity after myocardial infarctionCytotherapy34588150Cells were kept cold on ice until administration to animals. BM-MSCs were purchased from AllCells (Alameda, CA, USA). Cryopreserved BM-MSCs were washed twice in DMEM and resuspended at the same density as Cymerus MSC derivatives (5 × 106 cells per 10Full Paper
2021Kavanagh, H;Dunne, S;Martin, DS;McFadden, E;Gallagher, L;Schwaber, J;Leonard, S;O'Dea, S;A novel non-viral delivery method that enables efficient engineering of primary human T cells for ex vivo cell therapy applicationsCytotherapy33941482cells with minimal perturbation of cell function. Methods Ethics All experimental methods were carried out in accordance with the approved guidelines. Leukopaks from healthy donors were sourced from AllCells (Quincy, MA, USA) or BioIVT (Westbury, NYFull Paper
2021Wang, Y;Zhong, K;Ke, J;Chen, X;Chen, Y;Shu, W;Chen, C;Hu, S;Sun, X;Huang, H;Luo, C;Liu, L;Yang, J;Zhang, Y;Zhi, H;Combined 4-1BB and ICOS co-stimulation improves anti-tumor efficacy and persistence of dual anti-CD19/CD20 chimeric antigen receptor T cellsCytotherapy33863641Human peripheral blood mononuclear cells (PBMCs) from healthy donors were obtained from AllCells. The cryopreserved PBMCs were thawed into the T-cell culture medium and SaveFull Paper
2021Martin, A;Daris, M;Johnston, JA;Cui, J;HLA-A*02:01-directed chimeric antigen receptor/forkhead box P3-engineered CD4+ T cells adopt a regulatory phenotype and suppress established graft-versus-host diseaseCytotherapy33309258A*02:01- peripheral blood mononuclear cells (PBMCs) from four donors were isolated from fresh Leukopak (AllCells) using Ficoll density gradient media. CD4+ T cells or Tregs were SaveFull Paper
2021Story, JY;Zoine, JT;Burnham, RE;Hamilton, JAG;Spencer, HT;Doering, CB;Raikar, SS;Bortezomib enhances cytotoxicity of ex vivo-expanded gamma delta T cells against acute myeloid leukemia and T-cell acute lymphoblastic leukemiaCytotherapy33168453PBMCs (50-100 million per donor) from six different healthy donors were obtained through AllCells ( Eight total expansions from PBMCs from AllCells were performed, with two separate SaveFull Paper
2021Banerjee, A;Lu, Y;Do, K;Mize, T;Wu, X;Chen, X;Chen, J;Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain DiseasesFrontiers in cellular neuroscience33897370ity for polarization. With whole-genome transcript profile, we also demonstrated that iMG cells were clustered closely with human microglia. Materials and Methods Materials PBMCs were purchased from AllCells, LLC (Alameda, CA, USA, #PB003F and #PB00Full Paper
2021Stem, C;Rodman, C;Ramamurthy, RM;George, S;Meares, D;Farland, A;Atala, A;Doering, CB;Spencer, HT;Porada, CD;Almeida-Porada, G;Investigating Optimal Autologous Cellular Platforms for Prenatal or Perinatal Factor VIII Delivery to Treat Hemophilia AFrontiers in cell and developmental biology34447745Bone marrow mononuclear cells from two different biological donors were purchased from AllCells (Alameda, CA, United States) and their purchase approved by the Office of Human SaveFull Paper
2021Han, Y;Chen, Q;Zhang, L;Dissanayaka, WL;Indispensable Role of HIF-1α Signaling in Post-implantation Survival and Angio-/Vasculogenic Properties of SHEDFrontiers in cell and developmental biology34368116e expression of target proteins that mediate adaptive metabolic mechanisms in both in vivo and in vitro survival and angiogenesis in SHED. Materials and Methods Cell Culture SHED were purchased from AllCells (Alameda, CA, United States) and culturedFull Paper
2021Yazawa, T;Inaba, H;Imamichi, Y;Sekiguchi, T;Uwada, J;Islam, MS;Orisaka, M;Mikami, D;Ida, T;Sato, T;Miyashiro, Y;Takahashi, S;Khan, MRI;Suzuki, N;Umezawa, A;Kitano, T;Profiles of 5α-Reduced Androgens in Humans and Eels: 5α-Dihydrotestosterone and 11-Ketodihydrotestosterone Are Active Androgens Produced in Eel GonadsFrontiers in endocrinology33833737healthy women volunteers (obtaining informed consent) at University of Fukui Hospital in 2007. Plasma was separated by centrifugation at 1000g for 5 minutes. Other plasma samples were purchased from AllCells (Alameda, CA, USA) and ProMedDX (Norton, MFull Paper
2021Sternjak, A;Lee, F;Thomas, O;Balazs, M;Wahl, J;Lorenczewski, G;Ullrich, I;Muenz, M;Rattel, B;Bailis, JM;Friedrich, M;Preclinical Assessment of AMG 596, a Bispecific T-cell Engager (BiTE) Immunotherapy Targeting the Tumor-specific Antigen EGFRvIIIMolecular cancer therapeutics33632870Human pan-T cells (AllCells) were added at an E:T ratio of 10:1 with a dose range of AMG 596. Microvesicles were added simultaneously with T cells and AMG 596 to maintain SaveFull Paper
2021Massimino, M;Tirrò, E;Stella, S;Manzella, L;Pennisi, MS;Romano, C;Vitale, SR;Puma, A;Tomarchio, C;Di Gregorio, S;Antolino, A;Di Raimondo, F;Vigneri, P;Impact of the Breakpoint Region on the Leukemogenic Potential and the TKI Responsiveness of Atypical BCR-ABL1 TranscriptsFrontiers in pharmacology34276365CD34-positive cells derived from healthy donors were obtained from ALLCELLS. CD34-positive cells were maintained in Stem Span SFEM supplemented with cytokines at low SaveFull Paper
2021Tang, J;Zou, Y;Li, L;Lu, F;Xu, H;Ren, P;Bai, F;Niedermann, G;Zhu, X;BAY 60-6583 Enhances the Antitumor Function of Chimeric Antigen Receptor-Modified T Cells Independent of the Adenosine A2b ReceptorFrontiers in pharmacology33776765ed States) + 10% FBS (10099141C, ThermoFisher Scientific) were used to expand and culture CAR T cells. Frozen human peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from AllCells (PB005F, Alameda, CA, United States). AftFull Paper
2021Sim, DS;Mallari, CR;Teare, JM;Feldman, RI;Bauzon, M;Hermiston, TW;In vitro characterization of CT-001-a short-acting factor VIIa with enhanced prohemostatic activityResearch and practice in thrombosis and haemostasis34263099e binding of FVIIa to activated platelet surfaces was detected by Alexa488‐anti‐FVII (clone AA3). Donor informed consent and collection protocols are approved by the Institutional Review Board of AllCells. 2.4 Inhibition by antithrombin and tissue fFull Paper
2021Xu, Y;Barauskas, O;Kim, C;Babusis, D;Murakami, E;Kornyeyev, D;Lee, G;Stepan, G;Perron, M;Bannister, R;Schultz, BE;Sakowicz, R;Porter, D;Cihlar, T;Feng, JY;Off-Target In Vitro Profiling Demonstrates that Remdesivir Is a Highly Selective Antiviral AgentAntimicrobial agents and chemotherapy33229429Normal human primary bone marrow (BM) light-density cells were from three different lots obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD). All primary human cells SaveFull Paper
2021Huang, Y;Jia, F;Zhao, J;Hou, Y;Hu, SQ;Novel ACE Inhibitory Peptides Derived from Yeast Hydrolysates: Screening, Inhibition Mechanisms and Effects on HUVECsJournal of agricultural and food chemistry33593053Primary HUVECs, as well as the endothelial growth medium (EGM) and endothelial basal serum-free medium, were purchased from Allcells Co., Ltd. (Shanghai, China). Penicillin/ SaveFull Paper
2021Jing, L;Fan, S;Yao, X;Zhang, Y;Effects of compound stimulation of fluid shear stress plus ultrasound on stem cell proliferation and osteogenesisRegenerative biomaterials34868635lopment kit was purchased from Beyotime Biotechnology Co., Ltd. (China). The MagneSil@ Total RNA mini isolation system was purchased from Promega (USA). Primary rat BMSCs were purchased from Shanghai AllCells Biotech Co., Ltd. (China). The polytetrafFull Paper
2021Chai, ZT;Zhang, XP;Ao, JY;Zhu, XD;Wu, MC;Lau, WY;Sun, HC;Cheng, SQ;AXL Overexpression in Tumor-Derived Endothelial Cells Promotes Vessel Metastasis in Patients With Hepatocellular CarcinomaFrontiers in oncology34123800Primary human umbilical vein endothelial cells (HUVECs; AllCells, Shanghai, China) were cultured in endothelial cell growth medium-2 (Lonza, Basel, Switzerland) supplemented with SaveFull Paper
2021Romito, M;Juillerat, A;Kok, YL;Hildenbeutel, M;Rhiel, M;Andrieux, G;Geiger, J;Rudolph, C;Mussolino, C;Duclert, A;Metzner, KJ;Duchateau, P;Cathomen, T;Cornu, TI;Preclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV-1 InfectionBiotechnology journal33103367and data analyzed with CRISPResso2.[49] p values were adjusted with the Benjamini & Hochberg[50] method. For OCA, 1 × 106 PBMCs (ALLCELLS, USA)/mL in X-Vivo15 medium (Lonza), supplemented with 5% human AB serum (Gemini, USA) and 20 ng mL−1 of IL-2 (MFull Paper
2021Kitanaga, Y;Yamajuku, D;Kubo, S;Nakamura, K;Maeda, M;Seki, M;Kaneko, Y;Kinugasa, F;Morokata, T;Kondo, Y;Yoshinari, H;Nakayamada, S;Sumida, T;Tanaka, Y;Discovery of a novel Igβ and FcγRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosusInternational immunopharmacology34781122Human peripheral blood mononuclear cells (PBMCs; Lonza, Basel, Switzerland) and normal human peripheral blood B cells (AllCells, CA, USA) were purchased. Whole blood samples were obtained from healthy subjects and patients with SLE at the UniversityFull Paper
2021Chen, YL;Cui, Y;Liu, X;Liu, G;Dong, X;Tang, L;Hung, Y;Wang, C;Feng, MQ;A bispecific antibody targeting HER2 and PD-L1 inhibits tumor growth with superior efficacyThe Journal of biological chemistry34798072man IgG (H + L) was obtained from Jackson ImmunoResearch. Monocyte purification kit was obtained from Miltenyi Biotec. Ficoll gradient was obtained from GE healthcare. Human PBMCs were obtained from Allcells. Humanization of anti-PD-L1 mAb by framewFull Paper
2021Langrish, CL;Bradshaw, JM;Francesco, MR;Owens, TD;Xing, Y;Shu, J;LaStant, J;Bisconte, A;Outerbridge, C;White, SD;Hill, RJ;Brameld, KA;Goldstein, DM;Nunn, PA;Preclinical Efficacy and Anti-Inflammatory Mechanisms of Action of the Bruton Tyrosine Kinase Inhibitor Rilzabrutinib for Immune-Mediated DiseaseJournal of immunology (Baltimore, Md. : 1950)33674445Human whole blood from healthy donors was purchased from AllCells and assayed for rilzabrutinib inhibition of IgE basophil activation. Whole blood was treated with ten 3-fold dilutions SaveFull Paper
2021Andrejeva, G;Capoccia, BJ;Hiebsch, RR;Donio, MJ;Darwech, IM;Puro, RJ;Pereira, DS;Novel SIRPα Antibodies That Induce Single-Agent Phagocytosis of Tumor Cells while Preserving T CellsJournal of immunology (Baltimore, Md. : 1950)33431660were differentiated from CD14+ monocytes purchased from Astarte Biologics or selected using Pan Monocyte Isolation Kit (Miltenyi Biotec) from PBMCs (AllCells and Hemacare). Monocytes were seeded onto 96-well, flat-bottom plates at 3 × 104 cells per wFull Paper
2021Chaerkady, R;Zhou, Y;Delmar, JA;Weng, SHS;Wang, J;Awasthi, S;Sims, D;Bowen, MA;Yu, W;Cazares, LH;Sims, GP;Hess, S;Characterization of Citrullination Sites in Neutrophils and Mast Cells Activated by Ionomycin via Integration of Mass Spectrometry and Machine LearningJournal of proteome research34008986For mast cell preparation, three donors were used. Briefly, leukocytes were collected from Leukopak (ALLCells) and mixed with Robo II Buffer (StemCell Technologies) at 1:4 and centrifuged at 500g for 15 mins. The pellet was resuspended in 1× VitalyseFull Paper
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2021Friedman, D;Dozor, AJ;Milner, J;D'Souza, M;Talano, JA;Moore, TB;Shenoy, S;Shi, Q;Walters, MC;Vichinsky, E;Parsons, SK;Braniecki, S;Moorthy, CR;Ayello, J;Flower, A;Morris, E;Mahanti, H;Fabricatore, S;Klejmont, L;van de Ven, C;Baxter-Lowe, LA;Cairo, MS;Stable to improved cardiac and pulmonary function in children with high-risk sickle cell disease following haploidentical stem cell transplantationBone marrow transplantation33958740Bioverativ, Sangamo, Veevo, Editas Medicine and is medical director for AllCells, Inc. Biosciences. SKP is a consultant to Seattle Genetics, also unrelated to this study. EV is a consultant for ApoPharma, on the advisory committee for Global Blood ThFull Paper
2021Song, D;Liu, X;Dong, C;Wang, Q;Sha, C;Liu, C;Ning, Z;Han, J;Liu, H;Zong, M;Zhao, Y;Li, Y;Liu, G;Shao, X;Dou, C;Two novel human anti-CD25 antibodies with antitumor activity inversely related to their affinity and in vitro activityScientific reports34824364The effector cells PBMCs (PB003F-C, ALLCELLS) were added and the mixture was incubated at 37 C for 5 h. After substrate was added, OD490 value was read on the microplate Save;The effector cells PBMCs (PB003F-C, ALLCELLS) were added and the mixtureFull Paper
2021Loo, J;Sicher, I;Goff, A;Kim, O;Clary, N;Alexeev, A;Sulchek, T;Zamarayeva, A;Han, S;Calero-Garcia, M;Microfluidic transfection of mRNA into human primary lymphocytes and hematopoietic stem and progenitor cells using ultra-fast physical deformationsScientific reports34725429Mobilized peripheral blood CD34-selected hematopoietic stem and progenitor cells (mPB CD34 + HSPCs, purchased from AllCells) were taken from liquid nitrogen freezer and placed SaveFull Paper
2021Wang, X;Negrou, E;Maloney, MT;Bondar, VV;Andrews, SV;Montalban, M;Llapashtica, C;Maciuca, R;Nguyen, H;Solanoy, H;Arguello, A;Przybyla, L;Moerke, NJ;Huntwork-Rodriguez, S;Henry, AG;Understanding LRRK2 kinase activity in preclinical models and human subjects through quantitative analysis of LRRK2 and pT73 Rab10Scientific reports34145320University of Dundee (https://mrcppureagents.dundee.ac.uk/). For the total Rab10 assay, recombinant Rab10 protein (LSBio, LS-G2178) and Rab8 protein (LS-G29310) were used. Human cryopreserved PBMCs (AllCells, PB005F) were thawed and cultured in RPMIFull Paper
2021Xue, X;De Leon-Tabaldo, A;Luna-Roman, R;Castro, G;Albers, M;Schoetens, F;DePrimo, S;Devineni, D;Wilde, T;Goldberg, S;Kinzel, O;Hoffmann, T;Fourie, AM;Thurmond, RL;Preclinical and clinical characterization of the RORγt inhibitor JNJ-61803534Scientific reports34040108mentary materials and methods). Human nTreg suppressive assay Frozen purified human CD4+CD25+ natural Treg cells (nTreg), monocyte-derived dendritic cells (DC) and CD4+CD25- T effector cells (Teff) (Allcells, LLC, Alameda, CA) were thawed and co-culFull Paper
2021Harris, KE;Lorentsen, KJ;Malik-Chaudhry, HK;Loughlin, K;Basappa, HM;Hartstein, S;Ahmil, G;Allen, NS;Avanzino, BC;Balasubramani, A;Boudreau, AA;Chang, K;Cuturi, MC;Davison, LM;Ho, DM;Iyer, S;Rangaswamy, US;Sankaran, P;Schellenberger, U;Buelow, R;Trinklein, ND;A bispecific antibody agonist of the IL-2 heterodimeric receptor preferentially promotes in vivo expansion of CD8 and NK cellsScientific reports34011961resuspended in 125 µL/well flow buffer and acquired on a BD FACSCelesta. Whole blood cytokine release assay Cytokine secretion was detected using fresh human whole blood (heparinized) obtained from AllCells. The following method was adapted from B.Full Paper
2021Tago, Y;Kobayashi, C;Ogura, M;Wada, J;Yamaguchi, S;Yamaguchi, T;Hayashi, M;Nakaishi, T;Kubo, H;Ueda, Y;Human amnion-derived mesenchymal stem cells attenuate xenogeneic graft-versus-host disease by preventing T cell activation and proliferationScientific reports33510297Human BMSC from healthy donors were purchased from AllCells (CA, USA). The culture conditions of AMSC and BMSC (ie, MSC) were determined based on previous studies 43 . In SaveFull Paper
2021Subhi, H;Husein, A;Mohamad, D;Nik Abdul Ghani, NR;Nurul, AA;Chitosan-Based Accelerated Portland Cement Promotes Dentinogenic/Osteogenic Differentiation and Mineralization Activity of SHEDPolymers34641172SHED employed in this study were obtained from ALLCELLS, USA (Catalog No. DP004F; lot number SHED092211-01). Upon being received, the cells were characterized using stem SaveFull Paper
2021Kim, OH;Park, JH;Son, JI;Yoon, OJ;Lee, HJ;Bone Marrow Mesenchymal Stromal Cells on Silk Fibroin Scaffolds to Attenuate Polymicrobial Sepsis Induced by Cecal Ligation and PuncturePolymers33946773y (FE-SEM; JSM 7500F, JEOL Ltd., Akishima, Tokyo, Japan). 2.3. MSC Preparation Human bone marrow MSCs were derived from the whole bone marrow of independent human donors (commercially available from AllCells, Alameda, CA, USA). The cells were culturFull Paper
2021Wang, X;Sandberg, ML;Martin, AD;Negri, KR;Gabrelow, GB;Nampe, DP;Wu, ML;McElvain, ME;Toledo Warshaviak, D;Lee, WH;Oh, J;Daris, ME;Chai, F;Yao, C;Furney, J;Pigott, C;Kamb, A;Xu, H;Potent, Selective CARs as Potential T-Cell Therapeutics for HPV-positive CancersJournal of immunotherapy (Hagerstown, Md. : 1997)34432728For human peripheral blood mononuclear cells (PBMCs), collection protocols and donor informed-consent were approved by an Institutional Review Board at Allcells. Allcells followed SaveFull Paper
2021Martin, AD;Wang, X;Sandberg, ML;Negri, KR;Wu, ML;Toledo Warshaviak, D;Gabrelow, GB;McElvain, ME;Lee, B;Daris, ME;Xu, H;Kamb, A;Re-examination of MAGE-A3 as a T-cell Therapeutic TargetJournal of immunotherapy (Hagerstown, Md. : 1997)33284140purchased from Allcells according to the method described by Garcia et al31 Collection protocols and donor informed-consent were approved by an institutional review board at Allcells. SaveFull Paper
2021Xu, F;Liu, X;Zhang, D;Zhao, F;Fan, Z;Hu, S;Mei, S;Huang, Y;Sun, H;Wei, L;Guo, L;Wang, J;Cen, S;Liang, C;Guo, F;The Engineered MARCH8-Resistant Vesicular Stomatitis Virus Glycoprotein Enhances Lentiviral Vector TransductionHuman gene therapy33678011Peripheral blood mononuclear cells (PBMCs) isolated from healthy donors were purchased from AllCells (Shanghai, China), and cultured in RPMI 1640 media containing 10% FBS ( SaveFull Paper
2021Chen, Z;Chen, Z;Xu, S;Zhang, Q;LncRNA SOX2-OT/miR-30d-5p/PDK1 Regulates PD-L1 Checkpoint Through the mTOR Signaling Pathway to Promote Non-small Cell Lung Cancer Progression and Immune EscapeFrontiers in GeneticsThe microporous 2-compartment co-culture system was applied for co-culture. Transfected NSCLC cells were inoculated in the upper chamber, while CD8+ T (PB009-3-C, ALLCELLS, Shanghai) cells were inoculated in the lower chamber, allowing direct contactFull Paper
2021Raskin, S;Van Pelt, S;Toner, K;Balakrishnan, PB;Dave, H;Bollard, CM;Yvon, E;Novel TCR-like CAR-T cells targeting an HLA∗0201-restricted SSX2 epitope display strong activity against acute myeloid leukemiaMolecular therapy. Methods & clinical development34729377Steady-state apheresis units were purchased from AllCells and peripheral blood mononuclear cells (PBMCs) were isolated using lymphoprep density gradient. Before cryopreserving Save;ng a phase I clinical trial to evaluate the safety and anti-tumor eFull Paper
2021Xue, L;Gao, X;Zhang, H;Tang, J;Wang, Q;Li, F;Li, X;Yu, X;Lu, Z;Huang, Y;Tang, R;Yang, W;Antiangiogenic antibody BD0801 combined with immune checkpoint inhibitors achieves synergistic antitumor activity and affects the tumor microenvironmentBMC cancer34686154#0025), 1% penicillin/streptomycin (GIBCO, Invitrogen Inc., Carlsbad, CA, USA, #15140-122) and 1% ECGS (ScienCell, #1052). For HUVEC Western blot, migration inhibition, and scratch assays, HUVECs (AllCells, Alameda, CA, USA) were maintained in HUVECFull Paper
2021Noaks, E;Peticone, C;Kotsopoulou, E;Bracewell, DG;Enriching leukapheresis improves T cell activation and transduction efficiency during CAR T processingMolecular therapy. Methods & clinical development33718517Peripheral blood leukapheresis products were obtained from the NHS or purchased as LeukoPaks from AllCells, Cellex, and Anthony Nolan as part of research study NREC ID 15/LO/ Save;omplex and expensive magnetic cell sorting. Materials and methods CuFull Paper
2021Green, R;Elliott, JL;Norona, W;Go, F;Nguyen, VT;Ge, J;Short, ML;Mulero, JJ;Zhong, C;Developmental validation of VeriFiler™ Plus PCR Amplification Kit: A 6-dye multiplex assay designed for casework samplesForensic science international. Genetics33740707Human genomic DNA used for the degraded DNA experiments was extracted from normal peripheral blood mononuclear cells (PBMC) of a male donor (ALLCELLS, Emeryville, CA) using a QiAamp DNA Blood Maxi Kit (Qiagen)Full Paper
2021Park, S;Huang, NWY;Wong, CXY;Pan, J;Albakr, L;Gu, J;Kang, L;Microstructured Hyaluronic Acid Hydrogel for Tooth Germ BioengineeringGels (Basel, Switzerland)34449604ovine serum (Thermo Fisher, Waltham, MA, USA), penicillin-Streptomycin solution with 10,000 units penicillin and 10 mg streptomycin / mL (Thermo Fisher, Waltham, MA, USA). The culture media for DPSC (AllCells, Alameda, CA, USA) were α-Minimum essentiFull Paper
2021Garbuzova-Davis, S;Boccio, KJ;Llauget, A;Shell, R;Hailu, S;Mustafa, H;Ehrhart, J;Sanberg, PR;Appel, SH;Borlongan, CV;Beneficial Effects of Transplanted Human Bone Marrow Endothelial Progenitors on Functional and Cellular Components of Blood-Spinal Cord Barrier in ALS MiceeNeuro34479980Cryopreserved human bone marrow CD34+ cells (hBM34+) and human bone marrow-derived endothelial progenitor cells (hBM-EPCs) were purchased from AllCells and CELPROGEN SaveFull Paper
2021Chu, J;Pieles, O;Pfeifer, CG;Alt, V;Morsczeck, C;Docheva, D;Dental follicle cell differentiation towards periodontal ligament-like tissue in a self-assembly three-dimensional organoid modelEuropean cells & materials34251657according to AllCells company regulations, further donor information such gender and exact age was not provided to the purchaser). The PDL-hTERT immortal cell line has already SaveFull Paper
2021Madanagopal, TT;Tai, YK;Lim, SH;Fong, CH;Cao, T;Rosa, V;Franco-Obregón, A;Pulsed electromagnetic fields synergize with graphene to enhance dental pulp stem cell-derived neurogenesis by selectively targeting TRPC1 channelsEuropean cells & materials33644848hDPSC culture The use of human cells was approved by the NUS Institutional Review Board (NUS2094/2015). hDPSC (DPF003, AllCells, Quincy, MA, USA) were cultured in basal growth medium (DMEM, Invitrogen), supplemented with 10 % FBS (Invitrogen) in theFull Paper
2021Wang, X;Martin, AD;Negri, KR;McElvain, ME;Oh, J;Wu, ML;Lee, WH;Ando, Y;Gabrelow, GB;Toledo Warshaviak, D;Sandberg, ML;Xu, H;Kamb, A;Extensive functional comparisons between chimeric antigen receptors and T cell receptors highlight fundamental similaritiesMolecular immunology34419823Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols SaveFull Paper
2021Zacharia, A;Harberts, E;Valencia, SM;Myers, B;Sanders, C;Jain, A;Larson, NR;Middaugh, CR;Picking, WD;Difilippantonio, S;Kirnbauer, R;Roden, RB;Pinto, LA;Shoemaker, RH;Ernst, RK;Marshall, JD;Optimization of RG1-VLP vaccine performance in mice with novel TLR4 agonistsVaccine33309485cells were also cultured in the presence of BECC470 or TLR4 agonists and the resulting upregulation of surface co-stimulatory markers was measured by flow cytometry (AllCells). SaveFull Paper
2021Liu, C;Yang, G;Zhou, M;Zhang, X;Wu, X;Wu, P;Gu, X;Jiang, X;Magnesium Ammonium Phosphate Composite Cell-Laden Hydrogel Promotes Osteogenesis and Angiogenesis In VitroACS omega33869925Human umbilical vein endothelial cells (HUVECs; eahy926) were purchased from AllCells and cultured in EBM. They were seeded in the upper chamber, while the lower chambers SaveFull Paper
2021Jangjoo, M;Goodman, SJ;Choufani, S;Trost, B;Scherer, SW;Kelley, E;Ayub, M;Nicolson, R;Georgiades, S;Crosbie, J;Schachar, R;Anagnostou, E;Grunebaum, E;Weksberg, R;An Epigenetically Distinct Subset of Children With Autism Spectrum Disorder Resulting From Differences in Blood Cell CompositionFrontiers in neurology33935932ed, B cell, CD4T, CD8T, monocyte, granulocytes, and NK. These blood cell types were those on which the Houseman method was originally trained, using peripheral blood leukocyte subtypes purchased from AllCells , LLC (Emeryville, CA) or sorted cells frFull Paper
2021Zhou, N;Chen, Y;Yang, L;Xu, T;Wang, F;Chen, L;Liu, J;Liu, G;LncRNA SNHG4 promotes malignant biological behaviors and immune escape of colorectal cancer cells by regulating the miR-144-3p/MET axisAmerican journal of translational research34786048The transfected CACO2 cells were co-cultured with CD4+ T cells (PB009-2-C, AllCells Biotechnology Co., Ltd., Shanghai, China), followed by the addition of PD-1 antibody (ab52587, Abcam, UK) and PD-L1 antibody (ab205921, Abcam, UK). CD4+ T cell isolatFull Paper
2021Horton, TM;Hoff, FW;van Dijk, A;Jenkins, GN;Morrison, D;Bhatla, T;Hogan, L;Romanos-Sirakis, E;Meyer, J;Carroll, WL;Qiu, Y;Wang, T;Mo, Q;Kornblau, SM;The effects of sample handling on proteomics assessed by reverse phase protein arrays (RPPA): Functional proteomic profiling in leukemiaJournal of proteomics33212251Normalization controls were commercially available normal adult CD34+ bone marrow cells (AllCells). Peripheral blood mononuclear cells (PBMC) were isolated using Lymphoprep SaveFull Paper
2021Khouri, J;Faiman, BM;Grabowski, D;Mahfouz, RZ;Khan, SN;Wei, W;Valent, J;Dean, R;Samaras, C;Jha, BK;Lazarus, H;Campagnaro, EL;Malek, E;Reed, J;Karam, MA;Hamilton, K;Fada, S;Kalaycio, M;Liu, H;Sobecks, R;Saunthararajah, Y;Chew, Y;Orloff, M;Reu, FJ;DNA methylation inhibition in myeloma: Experience from a phase 1b study of low-dose continuous azacitidine in combination with lenalidomide and low-dose dexamethasone in relapsed or refractory multiple myelomaSeminars in hematology33509443Normal bone marrow mononuclear cells for separation of CD138 positive and negative fractions of 3 healthy donors was obtained commercially from ALLCELLS and processed the SaveFull Paper
2021Schölzel, C;Blesius, V;Ernst, G;Goesmann, A;Dominik, A;Countering reproducibility issues in mathematical models with software engineering techniques: A case study using a one-dimensional mathematical model of the atrioventricular nodePloS one34280231This means that the release version of the AllCells model is not only executable in OpenModelica, but also in any of the over 100 tools that support the Functional Mock-up Interface (FMI SaveFull Paper
2021Dulay, RM;Valdez, BC;Li, Y;Chakrabarti, S;Dhillon, B;Kalaw, SP;Reyes, RG;Cabrera, EC;Cytotoxicity of Gymnopilus purpureosquamulosus extracts on hematologic malignant cells through activation of the SAPK/JNK signaling pathwayPloS one34048499esuspended in freezing medium (50% heat-inactivated fetal bovine serum, 40% RPMI, 10% DMSO), and cryopreserved in liquid nitrogen until used. Mononuclear cells from healthy donors were purchased from ALLCELLS LLC (Almeda, CA, USA). For extract exposuFull Paper
2021Caplan, HW;Prabhakara, KS;Toledano Furman, NE;Zorofchian, S;Martin, C;Xue, H;Olson, SD;Cox, CS;Human-derived Treg and MSC combination therapy may augment immunosuppressive potency in vitro, but did not improve blood brain barrier integrity in an experimental rat traumatic brain injury modelPloS one34038436Bone marrow-derived MSC were isolated from a commercially available fresh human bone marrow aspirate (AllCells, Alameda, CA) and expanded following established procedures [8, SaveFull Paper
2021Baik, R;Wyman, SK;Kabir, S;Corn, JE;Genome editing to model and reverse a prevalent mutation associated with myeloproliferative neoplasmsPloS one33661998Frozen human mobilized peripheral blood CD34+ HSPCs were purchased from AllCells and thawed according to manufacturer's instructions. CD34+ cells were cultured in StemSpan SaveFull Paper
2021Garbuzova-Davis, S;Boccio, KJ;Ehrhart, J;Sanberg, PR;Appel, SH;Borlongan, CV;Detection of endothelial cell-associated human DNA reveals transplanted human bone marrow stem cell engraftment into CNS capillaries of ALS miceBrain research bulletin33545308Cryopreserved human bone marrow CD34+ cells (hBM34+) and human bone marrow-derived endothelial progenitor cells (hBM-EPCs) were purchased from AllCells (Alameda, CA, USA) and CELPROGEN (Torrance, CA, USA), respectively. According to the company reporFull Paper
2021Montalban-Bravo, G;Darbaniyan, F;Kanagal-Shamanna, R;Ganan-Gomez, I;Class, CA;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, KA;Chien, KS;Bueso-Ramos, C;Do, KA;Kantarjian, H;Garcia-Manero, G;Type I interferon upregulation and deregulation of genes involved in monopoiesis in chronic myelomonocytic leukemiaLeukemia research33517186BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). Informed consent was obtained according to protocols approved by the MDACC institutional review SaveFull Paper
2021Lu, J;Collins, P;Lee, KJ;Li, CM;Wang, S;In vitro assessment of an engineered tBID-based safety switch system in human T lymphocytesAnnals of translational medicine34988150n 12-well plate and incubated the plate in incubator at 37 °C. Human T-cell samples were purchased anonymously from the vendor and considered exempt from IRB approval. The frozen human pan-T cells (AllCells, LLC., Cat# S-ISO004-06) were thawed and cFull Paper
2021Maucort, C;Di Giorgio, A;Azoulay, S;Duca, M;Differentiation of Cancer Stem Cells by Using Synthetic Small Molecules: Toward New Therapeutic Strategies against Therapy ResistanceChemMedChem32803855In an analogous work, it was found that ICG-001 could safely eradicate the drug-resistant CSC-like population and initiate cell differentiation in leukemia (pre-B ALLcells). The same SaveFull Paper
2021Hu, J;Zhang, J;Yu, M;Liu, Z;Zou, Y;Hong, L;Zhang, T;Sun, J;Zheng, M;Zhu, X;Wang, Z;Liu, S;Circulating miR-221/222 reduces CD4+ T cells by inhibiting CD4 expression in colorectal cancerActa biochimica et biophysica Sinica34357372Peripheral blood mononuclear cells (PBMCs) were purchased from ALLCELLS (PB005F; Shanghai, China). PBMCs were incubated with DynaBeads (11141D; Gibco, Carlsbad, USA), and CD3+ T cells were isolated in a magnet by binding to DynaBeads containing anti-Full Paper
2021Yang, Y;Wang, C;Dai, C;Liu, X;Li, W;Huang, M;Zhao, X;Ji, D;Li, J;Guo, W;Amplification and expression of c-MET correlate with poor prognosis of patients with gastric cancer and upregulate the expression of PDL1Acta biochimica et biophysica Sinica33693450The prognostic significance of c-MET in gastric cancer (GC) remains uncertain. In the present study, we examined the amplification, expression, and the prognostic value of c-MET, SaveFull Paper
2021Thorarensen, A;Balbo, P;Banker, ME;Czerwinski, RM;Kuhn, M;Maurer, TS;Telliez, JB;Vincent, F;Wittwer, AJ;The advantages of describing covalent inhibitor in vitro potencies by IC50 at a fixed time point. IC50 determination of covalent inhibitors provides meaningful data to medicinal chemistry for SAR optimizationBioorganic & medicinal chemistry33285410Cryopreserved human PBMCs (Catalog No. PB005F), which were used in the IL-2, IL-10, IL-15, and IFNα assays, were purchased from Allcells (Emeryville, CA). Frozen PBMCs were thawed in a water bath (37 °C), and washed once with RPMI1640 medium (CatalogFull Paper
2021Jiang, J;Li, X;Mao, F;Wu, X;Chen, Y;Small molecular fluorescence dyes for immuno cell analysisAnalytical biochemistry33306976Statement of Ethical Use of Human Samples: All human PBMCs used in this study were obtained from AllCells Alameda, 1301 Harbor Bay Parkway, Suite 200, Alameda, CA 94502, Save;h as leukemia; and provide a quick and efficient way to study the immuneFull Paper
2021Takahashi, N;Higa, A;Hiyama, G;Tamura, H;Hoshi, H;Dobashi, Y;Katahira, K;Ishihara, H;Takagi, K;Goda, K;Okabe, N;Muto, S;Suzuki, H;Shimomura, K;Watanabe, S;Takagi, M;Construction of in vitro patient-derived tumor models to evaluate anticancer agents and cancer immunotherapyOncology letters33841567patient, were used in this study. Patient-derived hematopoietic tumor cells were provided by Fukushima Medical University Hospital, the Japanese Pediatric Cancer Group ALL-R14 study, ProteoGenex, or AllCells. Peripheral blood mononuclear cells (PBMCsFull Paper
2021Yoshida, K;Takabayashi, T;Imoto, Y;Sakashita, M;Kato, Y;Narita, N;Fujieda, S;Increased Thrombin-Activatable Fibrinolysis Inhibitor in Response to Sublingual Immunotherapy for Allergic RhinitisThe Laryngoscope33844301Systems, Minneapolis, Minnesota ), 100 ng IL-13 (R&D Systems), 100 ng IL-4 plus 1 μg/mL TAFI (Abcam, Cambridge, UK), and 100 ng IL-13 with 1 μg/mL TAFI. Human mast cells were differentiated from CD34+ hematopoietic progenitor cells (ALLCELLS, AlamedFull Paper
2021Hashim, SNM;Yusof, MFH;Zahari, W;Noordin, KBAA;Akamatsu, T;Azlina, A;Amniotic membrane matrix effects on calcineurin-NFAT-related gene expressions of SHED treated with VEGF for endothelial differentiationIn vitro cellular & developmental biology. Animal34021476SHED (AllCells, Alameda, California) were cultured in a complete medium made of the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, Waltham, Massachusetts), supplemented with 15% of fetal bovine serum (FBS) (Gibco, Waltham, Massachusetts)Full Paper
2021Pieles, O;Hartmann, M;Morsczeck, C;AMP-activated protein kinase and the down-stream activated process of autophagy regulate the osteogenic differentiation of human dental follicle cellsArchives of oral biology332540472.1. Cell culture Human dental follicle cells were purchased from AllCells and cultivated in DMEM (Dulbecco’s Modified Eagle’s Medium) high glucose with 10 % FBS (fetal bovine serum) and 1% penicillin (10,000 units/mL) / streptomycin (10 mg/mL) solutFull Paper
2021Wang, Z;Jiang, L;Wang, J;Chai, Z;Xiong, W;Morphine promotes angiogenesis by activating PI3K/Akt/HIF-1α pathway and upregulating VEGF in hepatocellular carcinomaJournal of gastrointestinal oncology34532126Academy of Science (Shanghai, China). The cells were cultured in 10% FBS DMEM, 37 °C, 5% CO2 incubator. HUVECs were obtained from the Allcells Company(Shanghai, China) and cultured in 10% FBS Endothelial Cell Growth Medium (ECM, Lonza, Switzerland),Full Paper
2021Hayashi, Y;Kawaki, H;Hori, M;Shintani, K;Hasegawa, T;Tanaka, M;Kondoh, N;Yoshida, T;Kawano, S;Tamaki, Y;Evaluation of the mechanical properties and biocompatibility of gypsum-containing calcium silicate cementsDental materials journal33642445Evaluation of the cytotoxicity of cement-immersed media Samples of hDPSCs (Allcells, Emeryville, CA, USA), which had been subcultured 11-14 times in 10% fetal bovine serum (FBS)-containing α-MEM, were seeded within a 96-well plate at a density of 4,0Full Paper
2021Iwasa, M;Fujii, S;Fujishiro, A;Maekawa, T;Andoh, A;Takaori-Kondo, A;Ichinohe, T;Miura, Y;Impact of 2 Gy γ-irradiation on the hallmark characteristics of human bone marrow-derived MSCsInternational journal of hematology33386593Bone marrow of multiple healthy adults aged 21-44 years (#4362, #4615, and #4641) was purchased from AllCells (Emeryville, CA). Human BM-MSCs were isolated from bone marrow according to our previously published methods [12, 13]. In brief, a single-ceFull Paper
2021Cui, L;Yin, F;Cheng, J;Liu, H;Zheng, M;Liu, D;Wu, Z;Qian, Q;Optimized cytotoxicity assay for co-suspended effector and target cellsJournal of immunological methods34270976Peripheral blood mononuclear cells (PBMCs) obtained from whole blood of healthy volunteers (AllCells, Silicon Valley, CA, USA) were isolated by density gradient centrifugation with SaveFull Paper
2021Sunderic, K;Li, C;Ahmed, AHR;Dawkins, D;Azar, T;Cardoso, L;Wang, S;Tuning Thermal Dosage to Facilitate Mesenchymal Stem Cell Osteogenesis in Pro-Inflammatory EnvironmentJournal of biomechanical engineering32601701Human bone marrow explanted from the iliac crest of donors was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the RosetteSep SaveFull Paper
2021Koh, H;Kwon, SY;Zhen, X;Ha, HY;Lee, JH;Generation of induced pluripotent stem cell line (KRIBBi004-A) from adult bone marrow CD34+ cells from a patient carrying 46,XX,t(1;5)(p31.1;35.1) karyotypeStem cell research34736040Cell Source iPSC was derived from CD34+ bone marrow (BM) cells, obtained from AllCells, LLC Clonality Clonal Associated disease N/A Gene/locus Chromosome translocation t(1;5)(p31.1;35.1) Date archived/stock date Jun.2021 Cell line repository/bank TheFull Paper
2021Kim, J;Koh, H;Zhen, X;Lee, DS;Ha, HY;Lee, JH;Establishment of iPSC (KRIBBi001-A) from CD34+ group O D-negative bone marrow bloodStem cell research33529979Cell Source iPSC was derived from CD34+ bone marrow (BM) cells, obtained from AllCells, LLC Clonality Clonal Method of reprogramming Integration-free Sendai virus-based vectors (CytoTune™-iPS 2.0 Sendai Reprogramming Kit, Life Technologies)Full Paper
2021Fu, X;Shen, X;Yin, X;Zhang, Y;Wang, X;Han, Z;Lin, Q;Fan, J;Antioxidant and pro-apoptosis activities of coffee husk (Coffea arabica) anthocyaninsInternational Food Research JournalThe HUVEC and Caco-2 cell lines were purchased from ALLCELLS Biotechnology Company (Shanghai, China) and Kunming Institute of Zoology Cell Line Bank (Yunnan, China), respectively. Dulbecco’s Modified Eagle’s Medium (DMEM), foetal bovine serum, peniciFull Paper
2021Choi, J;Goulding, SP;Conn, BP;McGann, CD;Dietze, JL;Kohler, J;Lenkala, D;Boudot, A;Rothenberg, DA;Turcott, PJ;Srouji, JR;Foley, KC;Rooney, MS;van Buuren, MM;Gaynor, RB;Abelin, JG;Addona, TA;Juneja, VR;Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutationsCell reports methods35474673(HLA-A∗03:01, HLA-A∗11:01, HLA-A∗68:01, HLA-B∗07:02, HLA-C∗01:02, HLA-C∗03:04, and/or HLA-C∗08:02) were isolated using Ficoll separation from apheresis material (AllCells Save;130-050-201; RRID:AB_2665482 CD25 MicroBeads, human Miltenyi BiotecFull Paper
2021Cai, W;Dong, J;Gallolu Kankanamalage, S;Titong, A;Shi, J;Jia, Z;Wang, B;Huang, C;Zhang, J;Lin, J;Kan, SZ;Han, S;Zhou, J;Liu, Y;Biological activity validation of a computationally designed Rituximab/CD3 T cell engager targeting CD20+ cancers with multiple mechanisms of actionAntibody therapeutics34805746They were cultured in RPMI 1640 (Thermo fisher) medium supplemented with 10% fetal bovine serum (FBS) (GIBCO) at 37 °C, 5% CO2. Human peripheral blood mononuclear cells (hPBMC) were purchased from AllCells (Alameda, CA) and HumanCells Bio (San Jose,Full Paper
2021Moe, GR;Steirer, LM;Lee, JA;Shivakumar, A;Bolanos, AD;A cancer-unique glycan: de-N-acetyl polysialic acid (dPSA) linked to cell surface nucleolin depends on re-expression of the fetal polysialyltransferase ST8SIA2 geneJournal of experimental & clinical cancer research : CR34544457rting (FACS) binding, and fluorescence labeling experiments were treated with Accutase and washed with medium before use. Human normal peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Alameda, CA). Protein extraction from ceFull Paper
2021Xing, Y;Chu, KA;Wadhwa, J;Chen, W;Zhu, J;Bradshaw, JM;Shu, J;Foulke, MC;Loewenstein, N;Nunn, P;By, K;Phiasivongsa, P;Goldstein, DM;Langrish, CL;Preclinical Mechanisms of Topical PRN473, a Bruton Tyrosine Kinase Inhibitor, in Immune-Mediated Skin Disease ModelsImmunoHorizons34326199Human whole blood (AllCells) pretreated for 1 h with eleven 3-fold serial dilutions of PRN473, starting at a concentration of 10 μM, was incubated overnight with goat anti-human IgM F( SaveFull Paper
2021Pohlmeyer, CW;Shang, C;Han, P;Cui, ZH;Jones, RM;Clarke, AS;Murray, BP;Lopez, DA;Newstrom, DW;Inzunza, MD;Matzkies, FG;Currie, KS;Di Paolo, JA;Characterization of the mechanism of action of lanraplenib, a novel spleen tyrosine kinase inhibitor, in models of lupus nephritisBMC rheumatology33781343healthy donors or individuals with SLE (AllCells) were treated with lanraplenib or dimethyl Naive B cells were isolated from freshly collected PBMCs (AllCells) by negative selection ( SaveFull Paper
2021Nordin, NS;Mokhtar, KI;Mustafa, BE;Effects of Flaxseed (Linum usitatissimum) Extract on the Osteoblast Differentiation Potential of Stem Cells Derived from Human Exfoliated Deciduous TeethMakara Journal of Health ResearchSHED (ALLCells, USA) were cultured in complete growth media (alpha-MEM; 10% FBS; 0.5% Pen-Strep) until confluence. SHED at 80%-90% confluence were subjected to osteoblast differentiation. The complete growth medium was replaced with osteoblast inductFull Paper
2021Turkson, A;Addor, J;Kharib, D;Validating Intrinsic Factors Informing E-Commerce: Categorical Data Analysis DemoOpen Journal of Statisticsows a chi-square distribution, and the conclusion depends on whether or not the obtained statistic is greater that the critical statistic at a chosen alpha level. The test statistics is: χ 2 = ∑ allcells ( Observed − Expected ) 2 Expected = ∑ i , jFull Paper
2021Xu, Y;Shen, L;Li, G;The The Effect of Elution Volume for Immunoprecipitation on m6A-Seq AnalysisProceedings of Anticancer ResearchThe Mouse Renal Carcinoma cells (RenCa) were cultured in DMEM medium (Invitrogen) containing 10% FBS, 2 mM L-Glutamine and 1% penicillin-streptomycin. RenCa cell line was purchased from Allcells Biotechnology (Shanghai, China) and were routinely testFull Paper
2021Zhou, Z;Zheng, J;Lin, D;Chen, Y;Hu, X;Exosomes Derived from Dental Pulp Stem Cells Accelerate Cutaneous wound Healing by Enhancing Angiogenesis via Cdc42/p38 MAPK PathwayResearch Square1% penicillin-streptomycin (Sigma, USA). HUVECs were bought from ALLCELLS (Shanghai, China) and expanded in endothelial cell growth medium (EGM). For exosome co-cultures experiment, 10μg/ml of exosomes were added to the culture medium of HUVECs. AllFull Paper
2021Blitzer, G;Paz, C;Glassey, A;Ganz, O;Giri, J;Pennati, A;Meyers, R;Lunga, T;Robbins, D;Thibeault, S;Bates, A;Nickel, K;Morris, Z;Mattison, R;Chappell, R;Glazer, T;Rogus-Pulia, N;Galipeau, J;Kimple, R;A Pilot Study to Assess the Salivary Gland Regenerative Potential of Bone Marrow Mesenchymal Stromal Cells from Treated Head and Neck Cancer PatientsResearch SquareBone marrow from healthy adult control volunteers were purchased from a commercial source (AllCells, Alameda, CA). We had originally planned to enroll 15 patients: 5 patients with SaveFull Paper
2021Tie, B;Guo, Z;Li, L;Wang, W;Liu, R;Fang, J;miR-3156-5p Enhances Hypoxia-Induced Angiogenesis in Hepatocellular Carcinoma via Modulating the SOCS5/HIF-1α/VEGF PathwayResearch SquareHuman umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in the medium containing 50 U/mL penicillin and 50 U/mL streptomycin (Invitgen, San Diego, CA), 50 mL endothelial cell growth additive (UpstatFull Paper
2021Chen, S;Barrientos, J;Ferrer, G;Ravichandran, P;Ibrahim, M;Kieso, Y;Kutok, J;Peluso, M;Sharma, S;Weaver, D;Pachter, J;Rai, K;Chiorazzi, N;Duvelisib Eliminates CLL B Cells, Impairs CLL-Supporting Cells, and Overcomes Ibrutinib Resistance in Preclinical ModelsResearch SquareHuman PBMCs from CLL donors obtained from whole blood from a commercial source (Bioreclamation IVT or ALLCELLS) were resuspended (1 x 106 cells/mL) in RPMI-1640 medium SaveFull Paper
2021Valadkhan, S;Plasek, L;Gunawardane, L;Niazi, F;Mason, S;Leskov, K;Perez, G;Dobrowolski, C;Shukla, M;Nutt, W;Karn, J;An HIV-induced mechanism for T cell quiescence andproviral latencyResearch SquarePeripheral blood mononuclear cells from HIV negative Caucasian male donors were purchased from Allcells. Naive CD4+ T cells were isolated using EasySep Human Naive CD4+ T SaveFull Paper
2021Cai, W;Dong, J;Kankanamalage, S;Titong, A;Shi, J;Jia, Z;Wang, B;Huang, C;Zhang, J;Lin, J;Kan, S;Zhou, J;Liu, Y;A computationally designed Rituximab/CD3 T cell engager targeting CD20+ cancers with multiple mechanisms of actionResearch Squaremedium supplemented with 10% fetal bovine serum (FBS) (GIBCO) 115 at 37°C, 5% CO2. Human peripheral blood mononuclear cells (hPBMC) were purchased from 116 AllCells (Alameda, CA) and HumanCells Bio (San Jose, CA). Rituximab resistant Raji cells 117 (Full Paper
2021Zhao, C;Zhang, W;Gong, G;Xie, L;Wang, M;Hu, Y;A New Approach to Produce IgG4-like Bispecific AntibodiesResearch SquareHuman CD4+ T cells were purified from PBMC (AllCells, China) by negative selection with CD4+ T cell enrichment cocktail kit (Stemcell, Vancouver, Canada) according to the manufacturer’s instruction. Immature DC (iDC) was generated from monocytes by cFull Paper
2021Noto, P;Analysis of Liver X Receptor target gene expression across speciesDrexel UniversitycDNA from Human MTC™ Panels I and II were purchased from Clontech (catalog# 636742 and 636743) and subjected to real-time PCR. Whole blood from human donors was purchased from AllCells (catalog# WB001)Full Paper
2021Dunbar, A;Kim, D;Lu, M;Farina, M;Yang, J;Park, Y;Gobbo, F;Verachi, P;Martelli, F;Karzai, A;Xiao, W;Xia, L;Elmansy, N;Kleppe, M;Chen, Z;Xiao, Y;McGovern, E;Snyder, J;Krishnan, A;Hill, C;Cordner, K;Zouak, A;Salama, M;Yohai, J;Tucker, E;Chen, J;Zhou, J;McConnell, T;Koche, R;Rampal, R;Migliaccio, A;Fan, R;Levine, R;Hoffman, R;CXCL8/CXCR2 signaling mediates bone marrow fibrosis and represents a therapeutic target in myelofibrosisbioRxivPro-inflammatory signaling is a hallmark feature of human cancer, including in myeloproliferative neoplasms (MPNs), most notably myelofibrosis (MF). Dysregulated inflammatory SaveFull Paper
2021Kartha, V;Duarte, F;Hu, Y;Ma, S;Chew, J;Lareau, C;Earl, A;Burkett, Z;Kohlway, A;Lebofsky, R;Buenrostro, J;Functional Inference of Gene Regulation using Single-Cell Multi-OmicsbioRxivCD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from AllCells (see Table S1 for catalog numbers and donor information). Cells were quickly thawed in a 37°C water bath, rinsed with culture medium (Iscove’s ModifiedFull Paper
2021Kong, T;Laranjeira, A;Yang, K;Fisher, D;Yu, L;Wang, A;Ruzinova, M;Fowles, J;Allen, M;Celik, H;Challen, G;Huang, S;Oh, S;DUSP6 mediates resistance to JAK2 inhibition and drives leukemic progressionbioRxivgradient extraction and cryopreserved according to standard procedures. Additional BMMCs were purchased from AllCells (Alameda, California). A list of patient samples utilized in this study is provided in Table 1Full Paper
2021Qi, J;D’Souza, D;Dawson, T;Geanon, D;Stefanos, H;Marvin, R;Walker, L;Rahman, A;Multimodal Single-Cell Characterization of the Human Granulocyte LineagebioRxivFresh whole bone marrow was purchased from AllCells Inc. and delivered on the day of the experiment. The bone marrow was in a volume of 3mL and the sample was limited to 2 units/ SaveFull Paper
2021Kim, J;Schulte, A;Sarver, A;Angelos, M;Frantz, A;Forster, C;O’Brien, T;Cornax, I;O’Sullivan, M;Cheng, N;Lewellen, M;Oseth, L;Kumar, S;Bullman, S;Pedamallu, C;Goyal, S;Meyerson, M;Lund, T;Alfoldi, J;Breen, M;Lindblad-Toh, K;Dickerson, E;Kaufman, D;Modiano, J;Transcriptional and Functional Activity of Hemangiosarcoma Support Bone Marrow Nurse Cell OntogenybioRxivHuman BM-derived mesenchymal stromal cells (hBM-MSCs) were isolated from whole bone marrow purchased from AllCells (Emeryville, CA, USA) as previously described [24-27]. M2- SaveFull Paper
2021Maughon, T;Shen, X;Huang, D;Michael, A;Shockey, W;Andrews, S;McRae, J;Platt, M;Fernández, F;Edison, A;Stice, S;Marklein, R;Multi-omics characterization of mesenchymal stem/stromal cells for the identification of putative critical quality attributesbioRxivPBMCs (AllCells, Alameda CA) were thawed in RPMI media (RPMI, 20% FBS, 2mM L-glutamine, 50 U/mL penicillin, 50 μg/mL streptomycin) and cultured overnight at 37°C and 5% CO2. Prior to co-culture, PBMCs were labeled with CFSE (Supplemental Table 1, BioFull Paper
2021Jain, S;Rick, J;Joshi, R;Beniwal, A;Spatz, J;Chang, A;Nguyen, A;Sudhir, S;Chandra, A;Haddad, A;Wadhwa, H;Shah, S;Choi, S;Hayes, J;Wang, L;Yagnik, G;Costello, J;Diaz, A;Aghi, M;Identification of Cancer-Associated Fibroblasts in Glioblastoma and Defining Their Pro-tumoral EffectsbioRxivTHP-1 cells and monocytes isolated from peripheral blood (AllCells) run through the MojoSort Human CD14 Selection Kit were treated with 50 ng/μL PMA (phorbol myristate acetate) SaveFull Paper
2021Andrews, S;Klinker, M;Bauer, S;Marklein, R;Morphological Landscapes from High Content Imaging Identify Optimal Priming Strategies that Enhance MSC ImmunosuppressionbioRxivHuman bone marrow-derived MSCs were obtained from 4 different donors purchased from Lonza (Walkersville, MD, USA), AllCells (Emeryville, CA, USA), and RoosterBio (Frederick, MD, USA) (see Table S1 for donor information). MSC culture conditions for thFull Paper
2021Gu, C;Cao, X;Wang, Z;Hu, X;Yao, Y;Zhou, Y;Liu, P;Liu, X;Gao, G;Hu, X;Zhang, Y;Chen, Z;Gao, L;Peng, Y;Jia, F;Shan, C;Yu, L;Liu, K;Li, N;Guo, W;Jiang, G;Min, J;Zhang, J;Yang, L;Shi, M;Hou, T;Li, Y;Liang, W;Lu, G;Yang, C;Wang, Y;Xia, K;Xiao, Z;Xue, J;Huang, X;Chen, X;Ma, H;Song, D;Pan, Z;Wang, X;Guo, H;Liang, H;Yuan, Z;Guan, W;Deng, S;A human antibody with blocking activity to RBD proteins of multiple SARS-CoV-2 variants including B.1.351 showed potent prophylactic and therapeutic efficacy against SARS-CoV-2 in rhesus macaquesbioRxivPhage libraries were constructed using antibody gene fragments amplified from PBMCs of 50 healthy human subjects (Allcells, PB003F and PB003C) and total RNA from PBMCs ( SaveFull Paper
2021Fitzgerald, A;Marcisak, E;Nasir, A;Glasgow, E;Jablonski, S;Van Der Veken, P;Pearson, G;Fertig, E;Mace, E;Weiner, L;Fibroblast activation protein regulates natural killer cell migration, extravasation and tumor infiltrationbioRxivfrom AllCells with either CD56 positive selection or CD56 negative selection (Allcells, cat# T cells, B cells and monocytes were isolated from PBMCs (Allcells) using Mojosort magnetic SaveFull Paper
2021Brooks, J;Preclinical Dynamic Contrast Enhanced Imaging for Longitudinal Biophysical Assessments of the Healthy and Malignant Vasculature after RadiotherapyThesisC57BL/6J mice (Strain 000664, Jackson Laboratory, Bar Harbor, ME) mice were injected with 1x106 GFP+ BCR-ABL (p190Kd) expressing B-cell ALLcells [66], suspended in 200 µl of phosphate buffered saline, through the tail vein. To test drug delivery, injFull Paper
2021O'Connor, EI;Mechanisms Behind the Causal Role of Dietary Oxylipins of the Lipoxygenase Pathway in the Development of Pulmonary Arterial HypertensionThesisPAECs were incubated with or without 15-HETE and 12 hours later healthy CD8 cells (ALLCELLS, Lot# 3003028) were added. After 12 hours of contact between EC and CD8 cells, the cells were collected, fixed, and stained for cleaved caspase 3Full Paper
2021McIntyre, P;The Effects of a Pyk2 Kinase Inhibitor on the Proliferation and Differentiation of Human Dental Pulp Stem CellsThesisCommercially available human DPSCs, collected from the pulp of extracted teeth, have been previously purchased from ALLCells and stored in liquid nitrogen (Alameda, CA, USA). • SaveFull Paper
2021Noaks, E;Enhancing the consistency of donor derived peripheral blood mononuclear cells for CAR T-cell manufacturingThesisFor work completed at the company site healthy peripheral blood leukapheresis were obtained from the NHS or purchased as LeukoPaks from AllCells, Cellex and Anthony Nolan, as part of research study NREC ID 15/LO/1322. Experiments using healthy leukapFull Paper
2021Osman, ZF;Zahari, W;Kannan, TP;Ultrastructure changes in BMP-2-treated SHED cultured on glycerol preserved human amniotic membraneActa MicroscópicaommerciallysourcedSHED(AllCells,USA)wasculturedonde-epithelialized2cmx2cmglycerol-preserved γ-radiated AM in a culture dish containing 3 mlof media at different cell density for the respective days asthefollowing;2x105forday1,5x104forday7,2.5x104Full Paper
2021Luecken, MD;Burkhardt, DB;Cannoodt, R;A sandbox for prediction and integration of dna, rna, and proteins in single cellsConferenceWe sourced multiple samples of BMMCs from 10 donors via AllCells (California, USA), all healthy non-smokers without recent medical treatment. Donors varied by age (22 - 40), sex, SaveFull Paper
2021Vladimirova, T;Implementation and Evaluation of Verlet List-based Methods in AutoPasThesisInside the 2D vector for AllCells or 3D vector for CellPair respectively, the method adds the particle indices corresponding to the particle pointers from the same spot in the AoS neighbor SaveFull Paper
2021Maser, IP;In-depth characterization of myeloid enhanced humanized mouse strainsThesiswas diluted with 0.9% sodium chloride (Braun) and 15 mg/kg was intraperitoneally (i.p.) injected into mice. 24 hours later 1×105 human CD34+ umbilical cord blood-derived hematopoietic stem cells (HSCs) (Stem Cell Technologies/Allcells) in 100 µl phosFull Paper
2021Fitzgerald, AA;Enhancing Anti-Cancer Properties of Human Natural Killer CellsThesisFresh healthy donor NK cells were purchased from AllCells with either CD56 positive selection or CD56 negative selection (Allcells, cat#PB012-P or PB012-N). T cells, B cells and monocytes were isolated from PBMCs (Allcells) using Mojosort magnetic ceFull Paper
2021Butler, A;Computational Methods for the Integration of Single-Cell DataThesisWe therefore repeated the original experiment, where PBMCs from a healthy human donor (ALLCELLS) were cultured with RPMI medium supplemented with 10% FBS and stimulated for 6 h with IFNβ (100 U/ml, PBL Assay Science), with a subset of cells left unexFull Paper
2021Nataraja, S;Singh, M;Demes, S;Olson, L;Stanwix, J;Biddle, M;Clarke, E;Huang, Y;Nguyen, J;Chen, C;Zhang, P;Abdulla, F;Vercellotti, G;Belcher, J;ML-0207/ASP8731: A Novel BACH1 Inhibitor That Induces Fetal Hemoglobin in Treatment of Sickle Cell DiseaseBloodTranslational Development, Mitobridge, Cambridge, MA++Translational Science, Mitobridge, Cambridge, MA++Clinical Pharmacology and Exploratory Development, Astellas, Northbrook, IL++Translational Science, Mitobridge, Cambridge, MA++Translational ScienFull Paper
2021Qing, Y;Dong, L;Gao, L;Li, C;Li, Y;Han, L;Prince, E;Tan, B;Deng, X;Wetzel, C;Shen, C;Gao, M;Chen, Z;Li, W;Zhang, B;Braas, D;Ten Hoeve, J;Sanchez, GJ;Chen, H;Chan, LN;Chen, CW;Ann, D;Jiang, L;Müschen, M;Marcucci, G;Plas, DR;Li, Z;Su, R;Chen, J;R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axisMolecular cell33434505The Lin− progenitor cells were then subject to retroviral transduction with MSCV-Neo-MA9 construct by two rounds of ‘spinoculation’, as described previously (Li et al., 2015). After being selected for 7 days with 0.5 mg/mL G418 Sulfate (10131027, GibFull Paper
2021Li, C;Wang, H;Georgakopoulou, A;Gil, S;Yannaki, E;Lieber, A;In Vivo HSC Gene Therapy Using a Bi-modular HDAd5/35++ Vector Cures Sickle Cell Disease in a Mouse ModelMolecular therapy : the journal of the American Society of Gene Therapy32949495The lineage-minus (Lin−) cells were isolated by depletion of lineage-committed cells in bone marrow MNCs using the Mouse Lineage Cell Depletion Kit (Miltenyi Biotec, San Diego, CA, USA) according to the manufacturer’s instructions. CFU assays were peFull Paper
2021Loftus, JP;Yahiaoui, A;Brown, PA;Niswander, LM;Bagashev, A;Wang, M;Schauf, A;Tannheimer, S;Tasian, SK;Combinatorial efficacy of entospletinib and chemotherapy in patient-derived xenograft models of infant acute lymphoblastic leukemiaHaematologica32414848s Supplementary Appendix Acknowledgments We acknowledge Dr Ann Forslund and Ms Chelsea Mullins formerly at Gilead Sciences for study protocol management and data analysis, and Dr Emer Clarke at ReachBio Research Labs for assistance with experimFull Paper
2021DiLillo, DJ;Olson, K;Mohrs, K;Meagher, TC;Bray, K;Sineshchekova, O;Startz, T;Kuhnert, J;Retter, MW;Godin, S;Sharma, P;Delfino, F;Lin, J;Smith, E;Thurston, G;Kirshner, JR;A BCMAxCD3 bispecific T cell-engaging antibody demonstrates robust antitumor efficacy similar to that of anti-BCMA CAR T cellsBlood advances33651100T cells were purified from normal donor PBMCs (ReachBio) by using magnetic beads (Thermo Fisher Scientific) and activated with CD3/CD28 microbeads (Thermo Fisher Scientific) in SaveFull Paper
2021Byrgazov, K;Besse, A;Kraus, M;Slipicevic, A;Lehmann, F;Driessen, C;Besse, L;Novel Peptide-drug Conjugate Melflufen Efficiently Eradicates Bortezomib-resistant Multiple Myeloma Cells Including Tumor-initiating Myeloma Progenitor CellsHemaSphere34136753of MM were assessed in a semisolid methylcellulose-based media formulation containing 10% phytohemagglutinin (PHA)-stimulated leukocyte conditioned medium (ReachBio, WA). Save;cell outgrowth assay Clonogenic progenitors of MM were assessed in a semFull Paper
2021Swaminathan, M;Kopyt, N;Atta, MG;Radhakrishnan, J;Umanath, K;Nguyen, S;O'Rourke, B;Allen, A;Vaninov, N;Tilles, A;LaPointe, E;Blair, A;Gemmiti, C;Miller, B;Parekkadan, B;Barcia, RN;Pharmacological effects of ex vivo mesenchymal stem cell immunotherapy in patients with acute kidney injury and underlying systemic inflammationStem cells translational medicine34581517Flow cytometry testing of patient leukocytes was performed by ReachBio (Spokane, Washington). Blood was collected in CPT tubes (362 753; BD Biosciences), and peripheral blood Save;n a plasma preparation tube (362 788; BD Biosciences, San Jose, CaliFull Paper
2021Lin, M;Kowolik, CM;Xie, J;Yadav, S;Overman, LE;Horne, DA;Potent Anticancer Effects of Epidithiodiketopiperazine NT1721 in Cutaneous T Cell LymphomaCancers34282785TCC (Manassas, VA, USA), authenticated by STR-profiling at the source and passaged for less than 6 months after receipt or resuscitation. Peripheral blood mononuclear cells (PBMCs) were obtained from ReachBio (Spokane, WA, USA). All cells were grownFull Paper
2021Samuelson, C;Radtke, S;Zhu, H;Llewellyn, M;Fields, E;Cook, S;Huang, MW;Jerome, KR;Kiem, HP;Humbert, O;Multiplex CRISPR/Cas9 genome editing in hematopoietic stem cells for fetal hemoglobin reinduction generates chromosomal translocationsMolecular therapy. Methods & clinical development34853798cation event frequencies was also undertaken on each of these tissues from necropsy as per protocol above. CFU and HbF assays on mouse BM A total of 7 × 104/mL cells were plated in ColonyGEL 1402 (ReachBio, Seattle, WA) in triplicate from each mouseFull Paper
2021Sharifi, S;Sharifi, H;Akbari, A;Chodosh, J;Systematic optimization of visible light-induced crosslinking conditions of gelatin methacryloyl (GelMA)Scientific reports34857867and exposed to photoirradiation for a 4 and 10 min. Those cell-laden gels were immediately washed 3 times with PBS and incubated in 200 μL of an optimized serum-containing medium (catalog # 02,100, Reachbio lab, Spokane, WA) at 37 °C. Cell viabilityFull Paper
2021Haber, L;Olson, K;Kelly, MP;Crawford, A;DiLillo, DJ;Tavaré, R;Ullman, E;Mao, S;Canova, L;Sineshchekova, O;Finney, J;Pawashe, A;Patel, S;McKay, R;Rizvi, S;Damko, E;Chiu, D;Vazzana, K;Ram, P;Mohrs, K;D'Orvilliers, A;Xiao, J;Makonnen, S;Hickey, C;Arnold, C;Giurleo, J;Chen, YP;Thwaites, C;Dudgeon, D;Bray, K;Rafique, A;Huang, T;Delfino, F;Hermann, A;Kirshner, JR;Retter, MW;Babb, R;MacDonald, D;Chen, G;Olson, WC;Thurston, G;Davis, S;Lin, JC;Smith, E;Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuningScientific reports34257348ce with the ARRIVE guidelines. OVCAR-3 bioluminescence imaging (BLI) study Eight-week-old NOD SCID gamma chain knock-out (NSG) mice (Jackson Laboratory) were injected with 5 × 106 human PBMCs (ReachBio) before 1 × 106 ascites cells from the OVCAR-3/Full Paper
2021Patterson, AM;Sellamuthu, R;Plett, PA;Sampson, CH;Chua, HL;Fisher, A;Vemula, S;Feng, H;Katz, BP;Tudor, G;Miller, SJ;MacVittie, TJ;Booth, C;Orschell, CM;Establishing Pediatric Mouse Models of the Hematopoietic Acute Radiation Syndrome and the Delayed Effects of Acute Radiation ExposureRadiation research33577641elsewhere (47). Hematopoietic progenitor cell (HPC) number and function were quantitated from 1.0 × 10e5 low-density bone marrow (LDBM) in 1 ml ColonyGEL™ Mouse Complete Medium (ReachBio Research Labs, Seattle, WA)Full Paper
2021Rethnam, M;Tan, DQ;Suda, T;Myeloma cells self-promote migration by regulating TAB1-driven TIMP-1 expression in mesenchymal stem cellsBiochemical and biophysical research communications33183761Human BM MSCs (#02100, ReachBio) were grown in DMEM low glucose media (Biowest) supplemented with 10% FBS and 1% Antibiotic/Anti-mycotic (Gibco). Cells were passaged upon confluence and cultured at a density of 4.5 × 103 cells/cm2. U266-B1 (TIB-196,Full Paper
2021Andersen, A;Vieira-Brock, PL;Vaughan, B;Vollmer, D;Method development for the analysis of PBMC-mediated killing of K562 cells by bovine colostrumJournal of immunological methods34744024Declaration of Competing Interest Authors and principle investigators are employed by 4Life Research, which sells the test articles commercially. 4Life Research funded all portions of this study. Acknowledgements All testing was performed at ReachBiFull Paper
2021Radtke, S;Enstrom, M;Pande, D;Cui, M;Madhu, R;Perez, A;Kiem, H;Hematopoietic recovery after transplantation is primarily derived from the stochastic contribution of hematopoietic stem cellsbioRxivFor CFC assays, 800-1,200 sort-purified CD34+ cells and CD34-subpopulations were seeded into 3.5 ml ColonyGEL 1402 (ReachBio, Seattle, WA). Colonies were scored after 12 to 14 days, discriminating colony forming unit- (CFU-) granulocyte (CFU-G), macrFull Paper
2020Geoerger, B;Kang, HJ;Yalon-Oren, M;Marshall, LV;Vezina, C;Pappo, A;Laetsch, TW;Petrilli, AS;Ebinger, M;Toporski, J;Glade-Bender, J;Nicholls, W;Fox, E;DuBois, SG;Macy, ME;Cohn, SL;Pathiraja, K;Diede, SJ;Ebbinghaus, S;Pinto, N;Pembrolizumab in paediatric patients with advanced melanoma or a PD-L1-positive, advanced, relapsed, or refractory solid tumour or lymphoma (KEYNOTE-051): interim analysis of an open-label, single-arm, phase 1–2 trialLancet Oncol.31812554In all patients, baseline PD-L1 expression in tumour samples (positivity defined as any staining of the stroma or PD-L1 expression on at least 1% of tumour cells) was assessed centrally at QualTek Molecular Laboratories (Goleta, CA, USA) using a protFull Paper
2020Emens, LA;Esteva, FJ;Beresford, M;Saura, C;De Laurentiis, M;Kim, SB;Im, SA;Wang, Y;Salgado, R;Mani, A;Shah, J;Lambertini, C;Liu, H;de Haas, SL;Patre, M;Loi, S;Trastuzumab emtansine plus atezolizumab versus trastuzumab emtansine plus placebo in previously treated, HER2-positive advanced breast cancer (KATE2): a phase 2, multicentre, randomised, double-blind trialThe Lancet. Oncology33002436The KATE2 study is a randomised, double-blind, placebo-controlled, phase 2 trial at 68 centres from nine countries across Asia, Australia, North America, and western Europe (appendix pp 13–15). Eligible patients were adults (aged ≥18 years) with centFull Paper
2020Powell, SF;Gold, KA;Gitau, MM;Sumey, CJ;Lohr, MM;McGraw, SC;Nowak, RK;Jensen, AW;Blanchard, MJ;Fischer, CD;Bykowski, J;Ellison, CA;Black, LJ;Thompson, PA;Callejas-Valera, JL;Lee, JH;Cohen, EEW;Spanos, WC;Safety and Efficacy of Pembrolizumab With Chemoradiotherapy in Locally Advanced Head and Neck Squamous Cell Carcinoma: A Phase IB StudyJ. Clin. Oncol.32479189Testing was performed centrally by QualTek Laboratories (Santa Barbara, CA) using the PD-L1 IHC 22C3 pharmDx (Dako North America, Carpinteria, CA) IHC assay as previously described. 25 PD-L1 evaluation was performed using the combined proportional scFull Paper
2020Marabelle, A;Cassier, P;Fakih, M;Kao, S;Nielsen, D;Italiano, A;Guren, T;Dongen, M;Spencer, K;Bariani, G;Ascierto, P;Santoro, A;Hiret, S;Ott, P;Piha-Paul, S;Liu, C;Leiby, M;Norwood, K;Delord, J;Pembrolizumab for previously treated advanced anal squamous cell carcinoma: Pooled results from the KEYNOTE-028 and KEYNOTE-158 studies.JCOPD-L1 positivity). Baseline PD-L1 expression was assessed using a prototype IHC assay (QualTek) in KEYNOTE-028 and the PD-L1 IHC 22C3 pharmDx assay (Agilent Technologies) in KEYNOTE-158. Pts received pembroFull Paper
2020Greenstein, A;Munster, P;Sachdev, J;Fleming, G;Grauer, A;Shepherd, S;Impact of relacorilant, a selective glucocorticoid receptor antagonist, on the immunosuppressive effects of endogenous cortisol.JCOTina K. Schlafly of Corcept Therapeutics. Funding for editorial, design, and production support for this poster was provided by Corcept to MedVal Scientific Information Services, LLC, Princeton, NJ. Experiments were conducted at Ardigen SA, QualTek MFull Paper
2020Dudek, AZ;Liu, LC;Gupta, S;Logan, TF;Singer, EA;Joshi, M;Zakharia, YN;Lang, JM;Schwarz, JK;Al-Janadi, A;Alva, AS;Phase Ib/II Clinical Trial of Pembrolizumab With Bevacizumab for Metastatic Renal Cell Carcinoma: BTCRC-GU14-003J. Clin. Oncol.32097091Events (CTCAE; version 4.0). Exploratory Studies. Expression of PD-L1 in archived diagnostic tumor tissue was determined by immunohistochemistry (IHC) using a 22C3 antibody (Qualtek Electronics, Newton, PA). Both a modified ;logy Criteria for AdFull Paper
2020Tolaney, SM;Barroso-Sousa, R;Keenan, T;Li, T;Trippa, L;Vaz-Luis, I;Wulf, G;Spring, L;Sinclair, NF;Andrews, C;Pittenger, J;Richardson, ET;Dillon, D;Lin, NU;Overmoyer, B;Partridge, AH;Van Allen, E;Mittendorf, EA;Winer, EP;Krop, IE;Effect of Eribulin With or Without Pembrolizumab on Progression-Free Survival for Patients With Hormone Receptor-Positive, ERBB2-Negative Metastatic Breast Cancer: A Randomized Clinical TrialJAMA oncology32880602A total of 65 patients (74%) had PD-L1 testing assessed centrally by QualTek using the 22C3 antibody, and results were reported as a modified pro- portion score (MPS), defined as the proportion of cells, includ- ing both tumor and mononuclear inflammFull Paper
2020Zsiros, E;Lynam, S;Attwood, KM;Wang, C;Chilakapati, S;Gomez, EC;Liu, S;Akers, S;Lele, S;Frederick, PJ;Odunsi, K;Efficacy and Safety of Pembrolizumab in Combination With Bevacizumab and Oral Metronomic Cyclophosphamide in the Treatment of Recurrent Ovarian Cancer: A Phase 2 Nonrandomized Clinical TrialJAMA oncology33211063A modified proportion score threshold of at least 1% using the 22C3 antibody (QualTekMolecular Laboratories) was adopted similarly to previously described protocols.6Full Paper
2020Jabbour, SK;Berman, AT;Decker, RH;Lin, Y;Feigenberg, SJ;Gettinger, SN;Aggarwal, C;Langer, CJ;Simone, CB;Bradley, JD;Aisner, J;Malhotra, J;Phase 1 Trial of Pembrolizumab Administered Concurrently With Chemoradiotherapy for Locally Advanced Non-Small Cell Lung Cancer: A Nonrandomized Controlled TrialJAMA Oncol32077891Exploratory analyses included PD-L1 status, by mean proportion score. Immunohistochemical staining for PD-L1 used a mouse monoclonal anti-PD-L1 antibody (clone 22C3).21 Furthermore, TILs were scored on a continuum from 0 to 3 (0 indicates absent TILsFull Paper
2020Kelly, CM;Antonescu, CR;Bowler, T;Munhoz, R;Chi, P;Dickson, MA;Gounder, MM;Keohan, ML;Movva, S;Dholakia, R;Ahmad, H;Biniakewitz, M;Condy, M;Phelan, H;Callahan, M;Wong, P;Singer, S;Ariyan, C;Bartlett, EK;Crago, A;Yoon, S;Hwang, S;Erinjeri, JP;Qin, LX;Tap, WD;D'Angelo, SP;Objective Response Rate Among Patients With Locally Advanced or Metastatic Sarcoma Treated With Talimogene Laherparepvec in Combination With Pembrolizumab: A Phase 2 Clinical TrialJAMA Oncol31971541Paraffin-embedded tumor material taken before and after treatment was examined for TIL immune biomarker expression, including PD-L1, PD-1, CD3, CD4, FOXP3, CD8, CD68, CD163, and MHC1 by Qualtek Laboratory. Programmed death-ligand 1 tumor membranous eFull Paper
2020Mancuso, F;Ogbah, Z;Fasani, R;Lo Giacco, D;Ramos, L;Oaknin, A;Alsina, M;Elez, E;Mercade, T;Saura, C;Capdevila, J;Vieito, M;Felip, E;Dienstmann, R;Gros, A;Seoane, J;Nuciforo, P;Tabernero, J;Garralda, E;Vivancos, A;581P VHIO immune gene expression profiling (VIGex) panel, a tool to explore tumour immune microenvironmentAnnals of Oncologyself), Speaker Bureau/Expert testimony, Research grant/Funding (self), Travel/Accommodation/ Expenses: Novartis; Honoraria (self), Speaker Bureau/Expert testimony, Research grant/Funding (self), Travel/Accommodation/Expenses: Targos Molecular PatholoFull Paper
2020Jotte, R;Cappuzzo, F;Vynnychenko, I;Stroyakovskiy, D;Rodríguez-Abreu, D;Hussein, M;Soo, R;Conter, HJ;Kozuki, T;Huang, KC;Graupner, V;Sun, SW;Hoang, T;Jessop, H;McCleland, M;Ballinger, M;Sandler, A;Socinski, MA;Atezolizumab in Combination With Carboplatin and Nab-Paclitaxel in Advanced Squamous NSCLC (IMpower131): Results From a Randomized Phase III TrialJ Thorac Oncol32302702Skip to Main Content ;This work was supported by F. Hoffmann-La Roche Ltd/Genentech, Inc., a member of the Roche Group. The authors would like to acknowledge Targos Molecular Pathology GmbH (Kassel, Germany) for performing central PD-L1 and T-efFull Paper
2020M.C.Liu, G.R.Oxnard, E.A.Klein, C.Swanton, M.V.SeidenSensitive and specific multi-cancer detection and localization using methylation signatures in cell-free DNAAnnals of OncologyBackground: Early cancer detection could identify tumors at a time when outcomes are superior and treatment is less morbid. This prospective case-control sub-study (from NCT02889978 and NCT03085888) assessed the performance of targeted methylation anFull Paper
2020Taylor, WC;Comment on "Sensitive and specific multi-cancer detection and localization using methylation signatures in cell-free DNA"" by M. C. Liu et al"Ann. Oncol.32371122cfDNA [n = 2628 (1493 cancer; 1135 non-cancer)], formalin fixed paraffin embedded (FFPE) tumor biopsies (n = 242), and WBCs (n = 70) from the first CCGA sub-study; commercial tissue or cells [n = 227; Discovery Life Sciences (formerly Conversant BiolFull Paper
2020Draz, MS;Vasan, A;Muthupandian, A;Kanakasabapathy, MK;Thirumalaraju, P;Sreeram, A;Krishnakumar, S;Yogesh, V;Lin, W;Yu, XG;Chung, RT;Shafiee, H;Virus detection using nanoparticles and deep neural network-enabled smartphone systemScience advances33328239Deidentified HCV-infected and HBV-infected patient plasma/ serum samples were obtained from Discovery Life Sciences IncFull Paper
2020Chung, HC;Piha-Paul, SA;Lopez-Martin, J;Schellens, JHM;Kao, S;Miller, WH;Delord, JP;Gao, B;Planchard, D;Gottfried, M;Zer, A;Jalal, SI;Penel, N;Mehnert, JM;Matos, I;Bennouna, J;Kim, DW;Xu, L;Krishnan, S;Norwood, K;Ott, PA;Pembrolizumab After Two or More Lines of Previous Therapy in Patients With Recurrent or Metastatic SCLC: Results From the KEYNOTE-028 and KEYNOTE-158 StudiesJ Thorac Oncol31870883In KEYNOTE-028, tumor samples were assessed using a laboratory-developed prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA) with the 22C3 antibody clone (Merck Sharp & Dohme Corp., a subsidiary of Merck & Co., Inc., KenFull Paper
2020Freeman, ZT;Nirschl, TR;Hovelson, DH;Johnston, RJ;Engelhardt, JJ;Selby, MJ;Kochel, CM;Lan, RY;Zhai, J;Ghasemzadeh, A;Gupta, A;Skaist, AM;Wheelan, SJ;Jiang, H;Pearson, AT;Snyder, LA;Korman, AJ;Tomlins, SA;Yegnasubramanian, S;Drake, CG;A conserved intratumoral regulatory T cell signature identifies 4-1BB as a pan-cancer targetJ. Clin. Invest.32015231Study approval. All human tumors were collected with approval from the Johns Hopkins University IRB with the following protocol IDs: renal clear cell carcinoma (IRB00033839), prostate cancer (NA_00082175), bladder cancer (NA_00026693), and glioblastoFull Paper
2020Weiss, J;Sheth, S;Deal, AM;Grilley Olson, JE;Patel, S;Hackman, TG;Blumberg, JM;Galloway, T;Patel, S;Zanation, AM;Shen, CJ;Hayes, DN;Hilliard, C;Mehra, R;McKinnon, KP;Wang, HH;Weissler, MC;Bauman, JR;Chera, BS;Vincent, BG;Concurrent definitive immunoradiotherapy for patients with Stage III-IV Head and Neck Cancer and Cisplatin contraindicationClin. Cancer Res.32371539withdrawal of permission. Correlative Analysis PDL1 staining was conducted by Qualtek Molecular Laboratories (Newtown, PA) as previously described 5 and required by Merck in supported investigator initiated trials. For bothFull Paper
2020Graff, JN;Beer, TM;Alumkal, JJ;Slottke, RE;Redmond, WL;Thomas, GV;Thompson, RF;Wood, MA;Koguchi, Y;Chen, Y;Latour, E;Bergan, RC;Drake, CG;Moran, AE;A phase II single-arm study of pembrolizumab with enzalutamide in men with metastatic castration-resistant prostate cancer progressing on enzalutamide aloneJ Immunother Cancer32616555retained. Formalin-fixed, paraffin-embedded tumor samples were sent to a commercial vendor (Qualtek) for PD-L1 quantification by immunohistochemistry (clone 22C3). Additional methods details are described elsewhere.30Full Paper
2020Majd, N;Waguespack, S;Janku, F;Fu, S;Penas-Prado, M;Xu, M;Alshawa, A;Kamiya-Matsuoka, C;Raza, S;McCutcheon, I;Naing, A;Efficacy of pembrolizumab in patients with pituitary carcinoma: report of four cases from a phase II studyJournal for ImmunoTherapy of CancerPD-L1 staining was performed by Qualtek using Merck 22C3 antibody for PD-L1Full Paper
2020Lokeshwar, VB;Morera, DS;Hasanali, SL;Yates, TJ;Hupe, MC;Knapp, J;Lokeshwar, SD;Wang, J;Hennig, MJP;Baskar, R;Escudero, DO;Racine, RR;Dhir, N;Jordan, AR;Hoye, K;Azih, I;Manoharan, M;Klaassen, Z;Kavuri, S;Lopez, LE;Ghosh, S;Lokeshwar, BL;A Novel Splice Variant of HYAL-4 Drives Malignant Transformation and Predicts Outcome in Patients with Bladder CancerClin. Cancer Res.32094233Present address: Travis Yates: QualTek Molecular Laboratories, King of Prussia, PA; Diogo Escudero: George Washington University Kelly Hoye: University of North Carolina Lineberger Comprehensive Cancer Center; Ronny Racine: Astellas Institute for RegFull Paper
2020Fong, LY;Taccioli, C;Palamarchuk, A;Tagliazucchi, GM;Jing, R;Smalley, KJ;Fan, S;Altemus, J;Fiehn, O;Huebner, K;Farber, JL;Croce, CM;Abrogation of esophageal carcinoma development in miR-31 knockout ratsProc. Natl. Acad. Sci. U.S.A.32123074lia isolated using the DNeasy Blood & Tissue Kit (Qiagen). WGS. DNA library preparation, library quality control, and nonhuman WGS to yield ∼30× coverage were performed on the Illumina HiSeq X at HudsonAlpha Genomic Services Laboratory. WGS AnalysiFull Paper
2020Rodriguez, CP;Wu, QV;Voutsinas, J;Fromm, JR;Jiang, X;Pillarisetty, VG;Lee, SM;Santana-Davila, R;Goulart, B;Baik, CS;Chow, LQM;Eaton, K;Martins, R;A Phase II Trial of Pembrolizumab and Vorinostat in Recurrent Metastatic Head and Neck Squamous Cell Carcinomas and Salivary Gland CancerClin. Cancer Res.31796519Submission of archived tissue was required prior to study entry. In patient with no available archived tissue, fresh tissue biopsies were recommended. Biopsies were attempted after three cycles of therapy were completed, in patients who consented. PDFull Paper
2020Huetter, J;Gritzan, U;Gutcher, I;Doecke, WD;Luetke-Eversloh, MV;Golfier, S;Roider, HG;Frisk, AL;Hunter, J;Pow, A;Drake, A;Levine, Z;Levy, O;Azulay, M;Barbiro, I;Cojocaru, G;Vaknin, I;Kreft, B;Roese, L;Characterization of BAY 1905254, an immune checkpoint inhibitor targeting the immunoglobulin-like domain containing receptor 2 (ILDR2)Cancer Immunol Res32312711cancer patients, and commercially available cryopreserved human lymph node single- cell preparations from melanoma patients (Folio Conversant, USA) using the PrimeFlow™ RNA Assay technology (Invitrogen, CA, USA). Following enzymaticFull Paper
2020Ozkan-Dagliyan, I;Diehl, JN;George, SD;Schaefer, A;Papke, B;Klotz-Noack, K;Waters, AM;Goodwin, CM;Gautam, P;Pierobon, M;Peng, S;Gilbert, TSK;Lin, KH;Dagliyan, O;Wennerberg, K;Petricoin, EF;Tran, NL;Bhagwat, SV;Tiu, RV;Peng, SB;Herring, LE;Graves, LM;Sers, C;Wood, KC;Cox, AD;Der, CJ;Low-Dose Vertical Inhibition of the RAF-MEK-ERK Cascade Causes Apoptotic Death of KRAS Mutant CancersCell Rep32553168CRISPR sequencing was performed at UNC (High-Throughput Sequencing Facility, Chapel Hill, NC) and Hudson Alpha (HudsonAlpha Genomic Services Laboratory, Huntsville, AL)Full Paper
2020Wang, X;Kelkar, YD;Xiong, X;Martinson, EO;Lynch, J;Zhang, C;Werren, JH;Wang, X;Genome Report: Whole Genome Sequence and Annotation of the Parasitoid Jewel Wasp Nasonia giraulti Laboratory Strain RV2X[u]G3 (Bethesda)32571804and Helen Wisch Professorship. X.X. is supported by the Auburn University Presidential Graduate Research Fellowship and Auburn University College of Veterinary Medicine Dean’s Fellowship. We thank HudsonAlpha Discovery for assistance with Illumina seFull Paper
2020Ulasov, I;Borovjagin, A;Fares, J;Yakushov, S;Malin, D;Timashev, P;Lesniak, MS;MicroRNA 345 (miR345) regulates KISS1-E-cadherin functional interaction in breast cancer brain metastasesCancer Lett.32246957One hundred and eleven tumor samples, representing invasive ductal carcinoma (IDC) were subjected for evaluation as a part of brain tissue microarrays presented by: US Biomax (BR812), Folio Biosciences (AY-HH0137) and biobank at the University of ChiFull Paper
2020Chiang, J;Ahmed, N;Foote, J;Prasad, N;Karri, S;Pagidas, K;Wiltshire, A;Gunn, D;Singh, K;OVARIAN AGING AND REPRODUCTIVE SENESCENCE IN MOUSE MODEL OF MITOCHONDRIAL DYSFUNCTIONFertility and Sterility2 HudsonAlpha Discovery, Huntsville, AL; 3 TCM University, Lincoln, RIFull Paper
2020Ho, AY;Barker, CA;Arnold, BB;Powell, SN;Hu, ZI;Gucalp, A;Lebron-Zapata, L;Wen, HY;Kallman, C;D'Agnolo, A;Zhang, Z;Flynn, J;Dunn, SA;McArthur, HL;A phase 2 clinical trial assessing the efficacy and safety of pembrolizumab and radiotherapy in patients with metastatic triple-negative breast cancerCancer31747077PD‐L1 tumor expression was evaluated in prospectively collected samples of metastatic biopsies by a central laboratory (QualTek Molecular Laboratories, Goleta, California) using a previously described prototype assay using the 22C3 antibody (Merck anFull Paper
2020Kasimir-Bauer, S;Roder, J;Obermayr, E;Mahner, S;Vergote, I;Loverix, L;Braicu, E;Sehouli, J;Concin, N;Kimmig, R;Net, L;Roder, H;Zeillinger, R;Aust, S;On Behalf Of The Ovcad Ovarian Cancer Diagnosis Initiative, ;Definition and Independent Validation of a Proteomic-Classifier in Ovarian CancerCancers32899818These samples were obtained from commercial biobanks (Conversant Bio (Huntsville, AL, USA) and Oncology Metrics (Forth Worth, TX, USA)), under their ethics-approved protocolsFull Paper
2020Jimenez, C;Subbiah, V;Stephen, B;Ma, J;Milton, D;Xu, M;Zarifa, A;Akhmedzhanov, FO;Tsimberidou, A;Habra, MA;Rodon Anhert, J;Fu, S;Naing, A;Phase II Clinical Trial of Pembrolizumab in Patients with Progressive Metastatic Pheochromocytomas and ParagangliomasCancers32824391tissue samples or newly obtained biopsy specimens (if archival tissue was not available) were evaluated by immunohistochemical analysis to determine the expression of PDL-1 in tumor cells and tumor-infiltrating mononuclear inflammatory cells by QualtFull Paper
2020Jordan, AR;Wang, J;Yates, TJ;Hasanali, SL;Lokeshwar, SD;Morera, DS;Shamaladevi, N;Li, CS;Klaassen, Z;Terris, MK;Thangaraju, M;Singh, AB;Soloway, MS;Lokeshwar, VB;Molecular targeting of renal cell carcinoma by an oral combinationOncogenesis32427869Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, 1410 Laney Walker Blvd., Augusta, GA, 30912, USA.||Sheila and David Fuente Graduate Program in Cancer Biology, University of Miami-Miller School of MediFull Paper
2020Mehnert, JM;Bergsland, E;O'Neil, BH;Santoro, A;Schellens, JHM;Cohen, RB;Doi, T;Ott, PA;Pishvaian, MJ;Puzanov, I;Aung, KL;Hsu, C;Le Tourneau, C;Hollebecque, A;Élez, E;Tamura, K;Gould, M;Yang, P;Stein, K;Piha-Paul, SA;Pembrolizumab for the treatment of programmed death-ligand 1-positive advanced carcinoid or pancreatic neuroendocrine tumors: Results from the KEYNOTE-028 studyCancer32320048PD was confirmed by repeat radiographic imaging ≥4 weeks after the initial scan. Investigators could opt to keep clinically stable patients on treatment until confirmed PD. Safety was monitored through 30 days after the last treatment dose (90 days fFull Paper
2020Wen, X;Ou, Y;Zarick, H;Zhang, X;Hmelo, A;Victor, Q;Paul, E;Slocik, J;Naik, R;Bellan, L;Lin, E;Bardhan, R;PRADA: Portable Reusable Accurate Diagnostics with nanostar Antennas for multiplexed biomarker screeningBioeng Transl Medlabor intensive, and cost prohibitive.39,40 We chose to measure the accuracy of PRADA in commercially available de-identified human patient serum (Discovery Life Sciences Inc.) to recapitulate clinical diagnostics where biomarkers of interest competeFull Paper
2020Gonzalez-Ericsson, PI;Stovgaard, ES;Sua, LF;Reisenbichler, E;Kos, Z;Carter, JM;Michiels, S;Le Quesne, J;Nielsen, TO;Laenkholm, AV;Fox, SB;Adam, J;Bartlett, JM;Rimm, DL;Quinn, C;Peeters, D;Dieci, MV;Vincent-Salomon, A;Cree, I;Hida, AI;Balko, JM;Haynes, HR;Frahm, I;Acosta-Haab, G;Balancin, M;Bellolio, E;Yang, W;Kirtani, P;Sugie, T;Ehinger, A;Castaneda, CA;Kok, M;McArthur, H;Siziopikou, K;Badve, S;Fineberg, S;Gown, A;Viale, G;Schnitt, SJ;Pruneri, G;Penault-Llorca, F;Hewitt, S;Thompson, EA;Allison, KH;Symmans, WF;Bellizzi, AM;Brogi, E;Moore, DA;Larsimont, D;Dillon, DA;Lazar, A;Lien, H;Goetz, MP;Broeckx, G;El Bairi, K;Harbeck, N;Cimino-Mathews, A;Sotiriou, C;Adams, S;Liu, SW;Loibl, S;Chen, IC;Lakhani, SR;Juco, JW;Denkert, C;Blackley, EF;Demaria, S;Leon-Ferre, R;Gluz, O;Zardavas, D;Emancipator, K;Ely, S;Loi, S;Salgado, R;Sanders, M;, ;The path to a better biomarker: application of a risk management framework for the implementation of PD-L1 and TILs as immuno-oncology biomarkers in breast cancer clinical trials and daily practiceJ. Pathol.32129476PANACEA NCT02129556 58 Pembrolizumab + trastuzumab single arm phase Ib/II PreTx LAdv or mHER2+ BC Ib: PD‐L1+ (6) II: PD‐L1+ & PD‐L1‐ (52) PD‐L1 (QualTek/ 22C3) tested prospectively at BTx (58). TILs were evaluated retrospectively (48). II: HFull Paper
2020Lu, H;Molony, RD;Chen, D;Jang, S;Wolf, B;Ewert, S;Flaherty, M;Xu, F;Isim, S;Shim, Y;Dornelas, C;Balke, N;Leber, XC;Scharenberg, M;Koelln, J;Choi, E;Ward, R;Johnson, J;Calzascia, T;Isnardi, I;Williams, JA;Lindenbergh, PL;van de Donk, NWCJ;Mutis, T;Huet, H;Lees, E;Meyer, MJ;Development of anti-CD32b antibodies with enhanced Fc function for the treatment of B and plasma cell malignanciesMol. Cancer Ther.32847974detect CD32b expression on primary cells and cell lines. Patient samples for CD32b expression analysis were purchased from Conversant Bio, now part of Discovery Life Science. 2B6 binding to normal human plasma cells fromFull Paper
2020Hutchins, B;Starling, GC;McCoy, MA;Herzyk, D;Poulet, FM;Dulos, J;Liu, L;Kang, SP;Fayadat-Dilman, L;Hsieh, M;Andrews, CL;Ayanoglu, G;Cullen, C;Malefyt, RW;Kastelein, RA;Saux, SL;Lee, J;Li, S;Malashock, D;Sadekova, S;Soder, G;van Eenennaam, H;Willingham, A;Yu, Y;Streuli, M;Carven, GJ;van Elsas, A;Biophysical and Immunological Characterization and In Vivo Pharmacokinetics and Toxicology in Nonhuman Primates of the Anti-PD-1 Antibody PembrolizumabMolecular cancer therapeutics32229606See Supplementary Data for additional details. Primary tumor cell culture assay Fresh non-small cell lung cancer (NSCLC) tumor tissues were collected and shipped overnight in AQIX media (AQIX) by collection providers (Folio Biosciences, Bio-Options,Full Paper
2020Smith, SD;Till, BG;Shadman, MS;Lynch, RC;Cowan, AJ;Wu, QV;Voutsinas, J;Rasmussen, HA;Blue, K;Ujjani, CS;Shustov, A;Cassaday, RD;Fromm, JR;Gopal, AK;Pembrolizumab with R-CHOP in previously untreated diffuse large B-cell lymphoma: potential for biomarker driven therapyBr. J. Haematol.32030732PD‐L1 immunohistochemistry was performed centrally by QualTek Molecular Laboratories using the mouse monoclonal antibody clone 22c3 by previously described methods (Supplementary Appendix, Section B) (Dolled‐Filhart et al. , 2016). Statistical considFull Paper
2020Vijayvergia, N;Dasari, A;Deng, M;Litwin, S;Al-Toubah, T;Alpaugh, RK;Dotan, E;Hall, MJ;Ross, NM;Runyen, MM;Denlinger, CS;Halperin, DM;Cohen, SJ;Engstrom, PF;Strosberg, JR;Pembrolizumab monotherapy in patients with previously treated metastatic high-grade neuroendocrine neoplasms: joint analysis of two prospective, non-randomised trialsBr. J. Cancer32152503For patients enrolled into FC, PD-L1 immunohistochemistry (IHC) assay was performed at Qualtek Research Laboratories on formalin-fixed, paraffin-embedded tissue sections using anti-PD-L1 monoclonal antibody clone 22C3 [Merck Research Laboratories]Full Paper
2020Schruf, E;Schroeder, V;Le, HQ;Schönberger, T;Raedel, D;Stewart, EL;Fundel-Clemens, K;Bluhmki, T;Weigle, S;Schuler, M;Thomas, MJ;Heilker, R;Webster, MJ;Dass, M;Frick, M;Stierstorfer, B;Quast, K;Garnett, JP;Recapitulating idiopathic pulmonary fibrosis related alveolar epithelial dysfunction in a human iPSC-derived air-liquid interface modelFASEB J.32297676Formalin-fixed and paraffin embedded lung samples from human IPF patients were purchased from Folio Biosciences (Powell, OH, US) under the regulatory conditions of the Boehringer Ingelheim corporate policy regarding the acquisition and use of human bFull Paper
2020Kang, J;Yeom, G;Jang, H;Park, C;Kim, M;Highly sensitive and universal detection strategy based on a colorimetric assay using target-specific heterogeneous sandwich DNA aptamerAnalytica Chimica ActaAll reagent concentrations were the same as those analyzed in the developed method. 2.8. Evaluation of clinical samples. We obtained patient samples containing NPs of influenza A and B viruses from Discovery Life Sciences, Inc (Huntsville, AL, USA)Full Paper
2020Seyger, M;Abramovits, W;Liljedahl, M;Hoejen, MN;Teng, J;Safety and efficacy of fixed-dose combination calcipotriol (50 μg/g) and betamethasone dipropionate (0.5 mg/g) cutaneous foam in adolescent patients (aged 12 to <17 years) with plaque psoriasis: results of a phase II, open-label trialJ Eur Acad Dermatol Venereol32074665W. Abramovits has received honoraria or fees for serving on advisory boards as a speaker and as a consultant, and has received grants as an investigator from AbbVie, Akros, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant BioFull Paper
2020Piha-Paul, SA;Oh, DY;Ueno, M;Malka, D;Chung, HC;Nagrial, A;Kelley, RK;Ros, W;Italiano, A;Nakagawa, K;Rugo, HS;de Braud, F;Varga, AI;Hansen, A;Wang, H;Krishnan, S;Norwood, KG;Doi, T;Efficacy and safety of pembrolizumab for the treatment of advanced biliary cancer: Results from the KEYNOTE-158 and KEYNOTE-028 studiesInt. J. Cancer32359091In KEYNOTE‐028, a prototype immunohistochemistry assay (QualTek, Goleta, California) was used to prospectively detect PD‐L1 expression, with positivity defined as membranous PD‐L1 expression in ≥1% of tumor and associated inflammatory cells or positiFull Paper
2020Agnello, G;Alters, SE;Rowlinson, SW;Preclinical safety and antitumor activity of the arginine-degrading therapeutic enzyme pegzilarginase, a PEGylated, cobalt-substituted recombinant human arginase 1Transl Res31954097CD8 IHC analysis of FFPE sections was performed by QualTek Molecular Laboratories (Goleta, Calif) and LC3B IHC analysis of FFPE sections was performed by GoPath Laboratories (Buffalo Grove, Ill). Statistical analysesFull Paper
2020Ross, AE;Hurley, PJ;Tran, PT;Rowe, SP;Benzon, B;Neal, TO;Chapman, C;Harb, R;Milman, Y;Trock, BJ;Drake, CG;Antonarakis, ES;A pilot trial of pembrolizumab plus prostatic cryotherapy for men with newly diagnosed oligometastatic hormone-sensitive prostate cancerProstate Cancer Prostatic Dis.31611635pathologist. Post-treatment biopsies were evaluated for PD-L1, CD4, CD8, and FoxP3 proteins by single-stain semiquantitative immunohistochemistry performed by QualTek Molecular and Clinical Laboratories (Santa Barbara, CA) ;re assessed by a geniFull Paper
2020Scheffler, M;Holzem, A;Kron, A;Nogova, L;Ihle, MA;von Levetzow, C;Fassunke, J;Wömpner, C;Bitter, E;Koleczko, S;Abdulla, DSY;Michels, S;Fischer, R;Riedel, R;Weber, JP;Westphal, T;Gerigk, U;Kern, J;Kaminsky, B;Randerath, W;Kambartel, KO;Merkelbach-Bruse, S;Büttner, R;Wolf, J;Co-occurrence of targetable mutations in Non-small cell lung cancer (NSCLC) patients harboring MAP2K1 mutationsLung Cancer32361034Reinhard Büttner: RB has been a consultant for Pfizer and Novartis regarding MET inhibitors and is a co-founder and CSO of Targos Molecular Pathology, Kassel GermanyFull Paper
2020Lee, EK;Xiong, N;Cheng, SC;Barry, WT;Penson, RT;Konstantinopoulos, PA;Hoffman, MA;Horowitz, N;Dizon, DS;Stover, EH;Wright, AA;Campos, SM;Krasner, C;Morrissey, S;Whalen, C;Quinn, R;Matulonis, UA;Liu, JF;Combined pembrolizumab and pegylated liposomal doxorubicin in platinum resistant ovarian cancer: A phase 2 clinical trialGynecol. Oncol.32771276Tumor infiltrating lymphocytes (TILs) were estimated as an overall average of the sample and reported on a scale of 0 (absent TILs) to 3 (profuse infiltration). PD-L1 and TIL analysis was performed at QualTek Molecular Laboraties, PAFull Paper
2020Frumovitz, M;Westin, SN;Salvo, G;Zarifa, A;Xu, M;Yap, TA;Rodon, AJ;Karp, DD;Abonofal, A;Jazaeri, AA;Naing, A;Phase II study of pembrolizumab efficacy and safety in women with recurrent small cell neuroendocrine carcinoma of the lower genital tractGynecol. Oncol.32534809Adverse events were assessed using the National Cancer Institute Common Terminology Criteria for Adverse Events version 4.03. When fresh or archived tissue was available, PD-L1 biomarker analysis was performed by Qualtek assay using Merck 22C3 antiboFull Paper
2020Gaillard, S;Broadwater, G;Andrews, W;Starr, M;Previs, R;Havrilesky, L;Davidson, B;Berchuck, A;Yi, J;Nixon, A;Secord, A;Pembrolizumab window study: Illuminating the immunologic landscape in gynecologic cancersGynecologic Oncologychanges in PD-L1 expression (by immunohistochemistry with monoclonal antibody clone 22C3 on FFPE specimens;l Qualtek, PA) scored using a quantitative modified proportion score (MPS)Full Paper
2020Pillarisetti, K;Powers, G;Luistro, L;Babich, A;Baldwin, E;Li, Y;Zhang, X;Mendonça, M;Majewski, N;Nanjunda, R;Chin, D;Packman, K;Elsayed, Y;Attar, R;Gaudet, F;Teclistamab is an active T cell-redirecting bispecific antibody against B-cell maturation antigen for multiple myelomaBlood Adv32956453Plasma samples were from normal subjects (n = 39) and patients with MM (n = 40); donors were acquired from Conversant Biologics and subjected to proteolysis and subsequent mass spectrometry using a reference multiple reaction monitoring sequence (YCNFull Paper
2020Svrzikapa, N;Longo, K;Prasad, N;Boyanapalli, R;Brown, J;Dorset, D;Yourstone, S;Powers, J;Levy, S;Morris, A;Vargeese, C;Goyal, J;Investigational assay for haplotype phasing of the huntingtin geneMolecular Therapy - Methods & Clinical Development4 1 Wave Life Sciences Ltd., Cambridge, MA, 02138 USA; 2 Department of Paediatrics, Medical 5 Sciences Division, University of Oxford, Oxford, OX3 9DU UK; 3 HudsonAlpha Discovery, 6 Discovery Life Sciences, Huntsville, AL, 35806 USA; 4 Genomic ServicFull Paper
2020Sebest, P;Fojt, L;Ostatna, V;Fojta, M;Danhel, A;Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent proteinBioelectrochemistry31855832electrode setup composed of: i) a bPGE or edge-plane PGE (ePGE, both Momentive) working electrode, together with ii) a refillable miniature Ag/AgCl/3M KCl reference electrode (eDAQ) placed into the salt bridge composed of a heat shrink Teflon tube (QFull Paper
2020Xicoy, H;Brouwers, JF;Wieringa, B;Martens, GJM;Explorative Combined Lipid and Transcriptomic Profiling of Substantia Nigra and Putamen in Parkinson's DiseaseCells32858884Total RNA samples were spectrophotometrically analyzed and their 260/280 ratio was typically above 1.9. RNA-seq directionality library preparation and sequencing with Poly (A) selection was performed by HudsonAlpha Genomic Services Laboratory (HuntsvFull Paper
2020Elgharably, H;Okamoto, T;Ayyat, KS;Niikawa, H;Meade, S;Farver, C;Chan, ER;Aldred, MA;McCurry, KR;Human Lungs Airway Epithelium Upregulate MicroRNA-17 and MicroRNA-548b in Response to Cold Ischemia and Ex Vivo ReperfusionTransplantation32590607We thank Tracey Bonfield, PhD from CWRU for performing the cytokines analysis. We thank Gerard J. Nuovo, MD, and Adel Mikhail, PhD from Phylogeny Inc. for performing the in situ hybridization assays of the microRNAFull Paper
2020Grigorieva, J;Asmellash, S;Net, L;Tsypin, M;Roder, H;Roder, J;Mass Spectrometry-Based Multivariate Proteomic Tests for Prediction of Outcomes on Immune Checkpoint Blockade Therapy: The Modern Analytical ApproachInt J Mol Sci3201294150] PSEA reference set NSCLC patients; samples obtained from commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrix (Fort Worth, TX) 100 Grigorieva et al., 2019 [51] 3. BDX008 Test and ICB Test BDX008Full Paper
2020Awty-Carroll, D;Ravella, S;Clifton-Brown, J;Robson, P;Using a Taguchi DOE to investigate factors and interactions affecting germination in Miscanthus sinensisSci Rep32005862All dishes were prepared and tested at the same time to limit extraneous sources of variation. The Taguchi design of experiment (DOE) was produced and primarily analysed using Qualtek-4 (Nutek inc., Michigan, USA) with secondary analysis in R34 ;FulFull Paper
2020Noto, PB;Sikorski, TW;Zappacosta, F;Wagner, CD;Montes de Oca, R;Szapacs, ME;Annan, RS;Liu, Y;McHugh, CF;Mohammad, HP;Piccoli, SP;Creasy, CL;Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissuesScientific reports33335114nd Treatment of Laboratory Animals and were reviewed by the Institutional Animal Care and Use Committee at GSK. Immunohistochemistry Both total and ADM-R225-hnRNP-A1 IHC assays were developed and at QualTek Molecular Laboratories using anti-hnRNP-A1Full Paper
2020Rüschoff, J;Lebeau, A;Sinn, P;Schildhaus, HU;Decker, T;Ammann, J;Künzel, C;Koch, W;Untch, M;Statistical modelling of HER2-positivity in breast cancer: Final analyses from two large, multicentre, non-interventional studies in GermanyBreast31918324Institut für Pathologie Nordhessen and Targos Molecular Pathology GmbH, Germaniastr. 7, D-34119, Kassel, Germany. Electronic address: rueschoff@patho-nordhessen.de.++Institut für Pathologie, Universitätsklinikum Hamburg-Eppendorf, Martinistr. 52, 202Full Paper
2020Emancipator, K;Keytruda and PD-L1: a Real-World Example of Co-development of a Drug with a Predictive BiomarkerThe AAPS journal33222057two clinical trial assays were used (Table I). The first, described in the literature as the "prototype assay" (4), was developed in-house at Merck & Co., Inc., Kenilworth, NJ, USA, and eventually placed and refined in an accredited clinical laboratoFull Paper
2020Gebauer, F;Krämer, M;Bruns, C;Schlößer, HA;Thelen, M;Lohneis, P;Schröder, W;Zander, T;Alakus, H;Buettner, R;Loeser, H;Quaas, A;Lymphocyte activation gene-3 (LAG3) mRNA and protein expression on tumour infiltrating lymphocytes (TILs) in oesophageal adenocarcinomaJ. Cancer Res. Clin. Oncol.32592066Reinhard Büttner: Co-Founder and Co-Owner of Targos Molecular Pathology Inc. Kassel/Germany. Hans Schlösser: Funding for Research from Astra Zeneca for research outside this study. Thomas Zander: BMS: advisory board, clinical trial; Novartis: advisorFull Paper
2020Wang, X;Jaimes, M;Gu, H;Shults, K;Putta, S;Sharma, V;Chow, W;Gogoi, P;Handique, K;Patterson, BK;Cell by cell immuno- and cancer marker profiling of non-small cell lung cancer tissue: Checkpoint marker expression on CD103+, CD4+ T-cells predicts circulating tumor cellsTranslational oncology33217647of a total of 72 biomarkers that result from using combinations of cellular markers in our algorithm to predict CTCs. Material and methods Samples Fresh tissues were obtained from 10 NSCLC cases by Folio Biosciences [Powell, OH] with paired peripherFull Paper
2020MacCarty, N;Bentson, S;Cushman, K;Au, J;Li, C;Murugan, G;Still, D;Stratification of particulate matter in a kitchen: A comparison of empirical to predicted concentrations and implications for cookstove emissions targetsEnergy for Sustainable Developmentthe top perimeter (Fig. 3). Small computer fans (Qualtek FAD1-06020CSHW11 rated at 1.44 W @12 V and 16 CFM) covered the holes at the top perimeter to force air out of the test kitchen at a constant rate. In Phase 1 of theFull Paper
2020Jia, Y;Liu, L;Shan, B;Future of immune checkpoint inhibitors: focus on tumor immune microenvironmentAnnals of translational medicine33145314The TNBC cohort in KEYNOTE-012 phase Ib trial enrolled 32 cases of heavily pre-treated patients with PD-L1 positivity in the stroma or PD-L1 ≥1% tumor cells using their Qualtek assayFull Paper
2020Sebest, P;Ostatna, V;Fojta, M;Danhel, A;Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particlesJournal of Electroanalytical Chemistryin three-electrode setup composed of: i) base plane PGE (bPGE,od 3.0 mm, Momentive) as working electrode, together with ii) refillable miniature Ag/AgCl/3 M KCl reference electrode (eDAQ) placed into the salt bridge composed of heat shrink Teflon tubFull Paper
2020Rehkaemper, J;Korenkov, M;Quaas, A;Rueschoff, J;Pamuk, A;Zander, T;Hillmer, AM;Buettner, R;Hoelscher, AH;Bruns, CJ;Loeser, H;Alakus, H;Schoemig-Markiefka, B;Amplification of KRAS and its heterogeneity in non-Asian gastric adenocarcinomasBMC Cancer32571252Institute of Pathology, University Hospital Cologne, Cologne, Germany. jan.rehkaemper@uk-koeln.de.++Department of General, Visceral and Cancer Surgery, University Hospital Cologne, Cologne, Germany.++Institute of Pathology, University Hospital ColognFull Paper
2020Montalban-Bravo, G;Class, CA;Ganan-Gomez, I;Kanagal-Shamanna, R;Sasaki, K;Richard-Carpentier, G;Naqvi, K;Wei, Y;Yang, H;Soltysiak, KA;Chien, K;Bueso-Ramos, C;Do, KA;Kantarjian, H;Garcia-Manero, G;Transcriptomic analysis implicates necroptosis in disease progression and prognosis in myelodysplastic syndromesLeukemia31719677BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). Patient characteristics are detailed in Supplementary Table S1. Informed consent was obtained SaveFull Paper
2020Arumugam, A;Faron, ML;Yu, P;Markham, C;Wu, M;Wong, S;A Rapid SARS-CoV-2 RT-PCR Assay for Low Resource SettingsDiagnostics (Basel, Switzerland)32987722No. 10006606) were purchased from Integrated DNA Technologies (Coralville, IA, USA). The clinical specimens of Influenza A (DLS16-85584), Influenza B (DLS15-33890), and RSV (KH19-00715) were obtained from Discovery Life Sciences Inc CachedFull Paper
2020Bio, S;Nunes, B;Acute effects of diclofenac on zebrafish: Indications of oxidative effects and damages at environmentally realistic levels of exposureEnviron. Toxicol. Pharmacol.32320907With the increasing awareness about the contamination of the aquatic environment by pharmaceuticals, there is a growing need to study their adverse effects on aquatic organisms. Diclofenac is a non-steroidal anti-inflammatory drug (NSAID), whose wideFull Paper
2020Piedade, F;Bio, S;Nunes, B;Effects of common pharmaceutical drugs (paracetamol and acetylsalicylic acid) short term exposure on biomarkers of the mussel Mytilus sppEnviron. Toxicol. Pharmacol.31704586Pharmaceutical drugs in the wild may pose significant risks to non-target exposed organisms. This situation is even more troublesome for coastal marine or estuarine environments, located in the vicinity of large human conglomerates, for which the putFull Paper
2020Lin, J;Song, AJ;Hoffman-Censits, J;Leiby, BE;Tuluc, M;Shaw, C;Harshyne, L;Kean, R;Bar-Ad, V;Den, RB;Hurwitz, MD;Louie, J;Philipose, S;Deshmukh, SP;Johnson, JM;Dicker, AP;Hooper, DC;Kelly, WK;Lu, B;A Pilot Study of Radiation Therapy in Combination With Pembrolizumab in Patients With Metastatic Renal Cell CancerAm. J. Clin. Oncol.31693508When possible preradiation and post- radiation biopsy material was compared. Biopsy samples were analyzed by a QualTek pathologist (King of Prussia, PA) and Merck 22C3 antibody was used to score PD-L1 membrane expressionFull Paper
2020Johnston, G;Ramsey, HE;Liu, Q;Wang, J;Stengel, KR;Sampathi, S;Acharya, P;Arrate, M;Stubbs, MC;Burn, T;Savona, MR;Hiebert, SW;Nascent transcript and single-cell RNA-seq analysis defines the mechanism of action of the LSD1 inhibitor INCB059872 in myeloid leukemiaGene32422235Samples were submitted to HudsonAlpha Genomic Services Laboratory for library preparation with Nugen Ovation RNA-Seq System V2 kit (cat# 7102-08) and sequencing on Illumina NovaSeq6000. Data analysis was performed as described above. 2.13 Data availaFull Paper
2020Lin, H;Damen, JE;Walasek, MA;Szilvassy, SJ;Turhan, AG;Louis, SA;Eaves, AC;Wognum, AW;Feeder-free and serum-free in vitro assay for measuring the effect of drugs on acute and chronic myeloid leukemia stem/progenitor cellsExp. Hematol.32798646CML. 1, Folio Conversant, 3.64 × 10 6, 4.9%, 81.2%, 15 × 10 3, 84, Male, Pre treatment, 112.2; 1764.4. 2, Folio Conversant, 3.17 × 10 6, 6.8%, 79.3%, 18.5 × 10 3, 70, Male, Pre treatment, 44.3; 2380.1. 3, Folio Conversant, 1.38 × 10 6, 18.8%, 78.7%,Full Paper
2020Barroso-Sousa, R;Krop, IE;Trippa, L;Tan-Wasielewski, Z;Li, T;Osmani, W;Andrews, C;Dillon, D;Richardson, ET;Pastorello, RG;Winer, EP;Mittendorf, EA;Bellon, JR;Schoenfeld, JD;Tolaney, SM;A Phase II Study of Pembrolizumab in Combination With Palliative Radiotherapy for Hormone Receptor-positive Metastatic Breast CancerClin. Breast Cancer32113750Clinical benefit is defined as complete response, partial response, or stable disease ≥ 24 weeks according to RECIST 1.1. Exploratory Biomarkers. Five patients had PD-L1 testing assessed centrally by QualTek using the 22C3 antibodyFull Paper
2020Patel, JJ;Levy, DA;Nguyen, SA;Knochelmann, HM;Day, TA;Impact of PD-L1 expression and human papillomavirus status in anti-PD1/PDL1 immunotherapy for head and neck squamous cell carcinoma-Systematic review and meta-analysisHead Neck31762164Oro Valley, Arizona). One study28 did not specify the assay utilized for PD‐L1 expression, but rather reported the laboratory where analysis occurred (QualTek Molecular Laboratories, Goleta, California). This lab confirmed theFull Paper
2020Adekanmbi, EO;Giduthuri, AT;Srivastava, SK;Dielectric Characterization and Separation Optimization of Infiltrating Ductal Adenocarcinoma via Insulator-DielectrophoresisMicromachines (Basel)32218322The DEP suspending medium (dextrose solution) was prepared and characterized as described in our previous article [38]. The prepared 100 mL suspending medium was divided into five separate beakers. Into each beaker, except the first, calculated volumFull Paper
2020Jasani, B;Bänfer, G;Fish, R;Waelput, W;Sucaet, Y;Barker, C;Whiteley, JL;Walker, J;Hovelinck, R;Diezko, R;Evaluation of an online training tool for scoring programmed cell death ligand-1 (PD-L1) diagnostic tests for lung cancerDiagn Pathol32303234Correspondence: rolf.diezko@targos-gmbh.de 2Advance - Training and Consulting Unit, Targos Molecular Pathology GmbH, Kassel, Germany Full list of author information is available at the end of the article Jasani et al ;Correspondence: rolf.diezkoFull Paper
2020Schöniger, S;Degner, S;Zhang, Q;Schandelmaier, C;Aupperle-Lellbach, H;Jasani, B;Schoon, HA;Tumor Infiltrating Lymphocytes in Pet Rabbit Mammary Carcinomas: A Study with Relevance to Comparative PathologyAnimals : an open access journal from MDPI328245211. Targos Molecular Pathology GmbH, Germaniastrasse 7, 34119 Kassel, Germany. 2. Institute of Veterinary Pathology, University of Leipzig, An den Tierkliniken, 04109 Leipzig, Germany. 3. Institute of Anatomy, Experimental Neurobiology CachedFull Paper
2020Schöniger, S;Schoon, HA;The Healthy and Diseased Equine Endometrium: A Review of Morphological Features and Molecular AnalysesAnimals : an open access journal from MDPI32260515Targos Molecular Pathology GmbH, Germaniastrasse 7, 34119 Kassel, Germany.++Institute of Veterinary Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig, GermanyFull Paper
2020Fish, EJ;Martinez-Romero, EG;DeInnocentes, P;Koehler, JW;Prasad, N;Smith, AN;Bird, RC;Circulating microRNA as biomarkers of canine mammary carcinoma in dogsJ. Vet. Intern. Med.32342546Extracted RNA was stored at −80°C until being shipped on dry ice to the Genomic Services Laboratory at HudsonAlpha Discovery for deep‐sequencing as previously described.14 Briefly, RNA libraries were processed extracted RNA using a NEBNext Small RNAFull Paper
2020Nuovo, G;A broad-based approach to differentiate CIN from its mimics: The utility of in situ hybridization and immunohistochemistryAnn Diagn Pathol32330660study was the review of 100 consecutive cases of formalin fixed, paraffin embedded tissues cervical biopsies done for either an abnormal Pap smear and/or a high risk HPV DNA positive result where the diagnosis was listed as CIN 1 or CIN 2. Folio BiosFull Paper
2020Inceer, H;Ozcan, M;Taxonomic evaluations on the anatomical characters of leaf and achene in Turkish Tripleurospermum with its relative Matricaria (Asteraceae)FloraBesides, both genera have appeared well separated in different and supported clades based on molecular phylogeny (Inceer et al., 2018)Full Paper
2020Roder, J;Net, L;Oliveira, C;Meyer, K;Asmellash, S;Kasimir-Bauer, S;Pass, H;Weber, J;Roder, H;Grigorieva, J;A proposal for score assignment to characterize biological processes from mass spectral analysis of serumClinical Mass SpectrometrySamples were obtained from the commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrics (Fort Worth, TX). b They were purchased from commercial biobanks, including Conversant Bio (Huntsville, AL), AdeptBio (Memphis, TN), and ProMedDFull Paper
2020Köhne, M;Mönnig, F;Papin, J;Schöniger, S;Tönissen, A;Rohn, K;Martinsson, G;Schoon, HA;Sieme, H;Effects of Hysteroscopic and Uterine Body Insemination on the Presence of Selected Immune Cells in the Equine EndometriumJ. Equine Vet. Sci.32534786Current affiliation: Targos Molecular Pathology GmbH, 34119 Kassel, GermanyFull Paper
2020Schildhaus, HU;[Immunohistochemistry-based predictive biomarkers for lung cancer]Pathologe31989233Interessenkonflikt. H.-U. Schildhaus erhielt Honorare von MSD, BMS, Abbvie, ZytoVision, Zytomed, Pfizer, Abbott Molecular, Novartis Oncology und Roche. Der Autor ist Board Member bzw. Assessor bei QuIP, NordiQC und UKNEQAS sowie Angestellter der TargFull Paper
2020Tsai, AG;Glass, DR;Juntilla, M;Hartmann, FJ;Oak, JS;Fernandez-Pol, S;Ohgami, RS;Bendall, SC;Multiplexed single-cell morphometry for hematopathology diagnosticsNature medicine32161403Healthy bone marrow samples from AllCells were ordered, delivered and processed the same day. Healthy bone marrow 1 and 2 refer to the AllCells 9878 and 10874 samples, SaveFull Paper
2020Reyes, M;Filbin, MR;Bhattacharyya, RP;Billman, K;Eisenhaure, T;Hung, DT;Levy, BD;Baron, RM;Blainey, PC;Goldberg, MB;Hacohen, N;An immune-cell signature of bacterial sepsisNature medicine32066974De-identified BMMC samples were purchased from AllCells or Stemcell Technologies. This study was approved by the Institutional Review Boards at the Broad Institute of MIT and SaveFull Paper
2020Travaglini, KJ;Nabhan, AN;Penland, L;Sinha, R;Gillich, A;Sit, RV;Chang, S;Conley, SD;Mori, Y;Seita, J;Berry, GJ;Shrager, JB;Metzger, RJ;Kuo, CS;Neff, N;Weissman, IL;Quake, SR;Krasnow, MA;A molecular cell atlas of the human lung from single-cell RNA sequencingNature33208946For bulk RNA-seq of canonical immune populations, whole blood from healthy human donors was obtained commericially (AllCells) in EDTA tubes. Patient tissues were obtained under SaveFull Paper
2020Link, JO;Rhee, MS;Tse, WC;Zheng, J;Somoza, JR;Rowe, W;Begley, R;Chiu, A;Mulato, A;Hansen, D;Singer, E;Tsai, LK;Bam, RA;Chou, CH;Canales, E;Brizgys, G;Zhang, JR;Li, J;Graupe, M;Morganelli, P;Liu, Q;Wu, Q;Halcomb, RL;Saito, RD;Schroeder, SD;Lazerwith, SE;Bondy, S;Jin, D;Hung, M;Novikov, N;Liu, X;Villaseñor, AG;Cannizzaro, CE;Hu, EY;Anderson, RL;Appleby, TC;Lu, B;Mwangi, J;Liclican, A;Niedziela-Majka, A;Papalia, GA;Wong, MH;Leavitt, SA;Xu, Y;Koditek, D;Stepan, GJ;Yu, H;Pagratis, N;Clancy, S;Ahmadyar, S;Cai, TZ;Sellers, S;Wolckenhauer, SA;Ling, J;Callebaut, C;Margot, N;Ram, RR;Liu, YP;Hyland, R;Sinclair, GI;Ruane, PJ;Crofoot, GE;McDonald, CK;Brainard, DM;Lad, L;Swaminathan, S;Sundquist, WI;Sakowicz, R;Chester, AE;Lee, WE;Daar, ES;Yant, SR;Cihlar, T;Clinical targeting of HIV capsid protein with a long-acting small moleculeNature32612233Human peripheral blood mononuclear cells (PBMCs) were collected from healthy volunteers under informed consent; their use was approved by an institutional review board at AllCells SaveFull Paper
2020Ferrari, S;Jacob, A;Beretta, S;Unali, G;Albano, L;Vavassori, V;Cittaro, D;Lazarevic, D;Brombin, C;Cugnata, F;Kajaste-Rudnitski, A;Merelli, I;Genovese, P;Naldini, L;Efficient gene editing of human long-term hematopoietic stem cells validated by clonal trackingNature biotechnology32601433(Biovision) and 50 nM UM171 (STEMCell Technologies), unless otherwise specified. G-CSF mPB CD34+ HSPCs were purified with the CliniMACS CD34 Reagent System (Miltenyi Biotec) from Mobilized Leukopak (AllCells) upon approval by the Ospedale San RaffaeFull Paper
2020Spindler, MJ;Nelson, AL;Wagner, EK;Oppermans, N;Bridgeman, JS;Heather, JM;Adler, AS;Asensio, MA;Edgar, RC;Lim, YW;Meyer, EH;Hawkins, RE;Cobbold, M;Johnson, DS;Massively parallel interrogation and mining of natively paired human TCRαβ repertoiresNature biotechnology32393905novel ACT. Online Methods Sourcing and Processing Human Materials De-identified peripheral blood mononuclear cells (PBMCs) in leukopaks were obtained from HLA-A*02:01 and HLA-A*24:02 healthy donors (AllCells, Alameda, CA, USA), under an InstitutionaFull Paper
2020Dinh, HQ;Eggert, T;Meyer, MA;Zhu, YP;Olingy, CE;Llewellyn, R;Wu, R;Hedrick, CC;Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone MarrowImmunity32814027Human bone marrow cells Fresh bone marrow samples from anonymous healthy adult donors were obtained from AllCells, Inc. (Alameda, CA). For information on donor characteristics, refer to Supplemental Table 1. Upon arrival, the fresh cells were immediaFull Paper
2020Glass, DR;Tsai, AG;Oliveria, JP;Hartmann, FJ;Kimmey, SC;Calderon, AA;Borges, L;Glass, MC;Wagar, LE;Davis, MM;Bendall, SC;An Integrated Multi-omic Single-Cell Atlas of Human B Cell IdentityImmunity32668225structive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com -------------------------Full Paper
2020Park, HB;Wei, Z;Oh, J;Xu, H;Kim, CS;Wang, R;Wyche, TP;Piizzi, G;Flavell, RA;Crawford, JM;Sulfamethoxazole drug stress upregulates antioxidant immunomodulatory metabolites in Escherichia coliNature microbiology32719505ndors supply Eurofins DiscoverX with primary human cells: Cell Applications, Inc., CellzDirect, Celsis-IVT, Leukolab, Life Technologies, Lonza, ScienCell, Hemacare Corporation, Stemcell Technologies, AllCells, Physician’s Plasma Alliance, Lifeline CeFull Paper
2020Di Genua, C;Valletta, S;Buono, M;Stoilova, B;Sweeney, C;Rodriguez-Meira, A;Grover, A;Drissen, R;Meng, Y;Beveridge, R;Aboukhalil, Z;Karamitros, D;Belderbos, ME;Bystrykh, L;Thongjuea, S;Vyas, P;Nerlov, C;C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid ProgenitorCancer cell32330454derbos et al., 2017) ------------------------- Biological Samples ------------------------- AEL patient samples (OX1164; AYL050; MKH048; STB115) MDSBio NA Normal adult human bone marrow AllCells NA ------------------------- CriticalFull Paper
2020Li, S;Chen, X;Wang, J;Meydan, C;Glass, JL;Shih, AH;Delwel, R;Levine, RL;Mason, CE;Melnick, AM;Somatic Mutations Drive Specific, but Reversible, Epigenetic Heterogeneity States in AMLCancer discovery32938585from our prior study(9), 5 of which were purchased from AllCells (Alameda, CA, USA) and 9 of which were isolated using magnetic bead positive selection for CD34+ (Miltenyi Biotec) from freshly collected bone marrow samples from individuals without knFull Paper
2020Zhou, Y;Han, C;Wang, E;Lorch, AH;Serafin, V;Cho, BK;Gutierrez Diaz, BT;Calvo, J;Fang, C;Khodadadi-Jamayran, A;Tabaglio, T;Marier, C;Kuchmiy, A;Sun, L;Yacu, G;Filip, SK;Jin, Q;Takahashi, YH;Amici, DR;Rendleman, EJ;Rawat, R;Bresolin, S;Paganin, M;Zhang, C;Li, H;Kandela, I;Politanska, Y;Abdala-Valencia, H;Mendillo, ML;Zhu, P;Palhais, B;Van Vlierberghe, P;Taghon, T;Aifantis, I;Goo, YA;Guccione, E;Heguy, A;Tsirigos, A;Wee, KB;Mishra, RK;Pflumio, F;Accordi, B;Basso, G;Ntziachristos, P;Posttranslational Regulation of the Exon Skipping Machinery Controls Aberrant Splicing in LeukemiaCancer discovery32444465Our study provides a new proof-of-principle for posttranslational regulation of splicing factors independently of mutations in aggressive T-cell leukemia. It further suggests a new drug SaveFull Paper
2020Tochigi, T;Miyamoto, T;Hatakeyama, K;Sakoda, T;Ishihara, D;Irifune, H;Shima, T;Kato, K;Maeda, T;Ito, T;Handa, H;Akashi, K;Kikushige, Y;Aromatase is a novel neosubstrate of cereblon responsible for immunomodulatory drug-induced thrombocytopeniaBlood32219443Human adult bone marrow and granulocyte colony-stimulating factor-mobilized peripheral blood samples were obtained from healthy donors or purchased from AllCells. Cord blood SaveFull Paper
2020Montalban Bravo, G;Kanagal-Shamanna, R;Darbaniyan, F;Ganan-Gomez, I;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, K;Chien, K;Bueso-Ramos, C;Kantarjian, H;Garcia-Manero, G;Genomic and Transcriptomic Differences of Myelodysplastic Syndrome/Myeloproliferative Neoplasm with Ring Sideroblasts and Thrombocytosis (MDS/MPN-RS-T) and Myelodysplastic Syndrome with Ring Sideroblasts (MDS-RS)BloodCD34+ cells from bone marrow samples of 4 pts with MDS/MPN-RS-T, 7 pts with MDS-RS and 17 healthy individuals obtained from AllCells (Emeryville, CA) were isolated using the SaveFull Paper
2020Montalban Bravo, G;Darbaniyan, F;Kanagal-Shamanna, R;Ganan-Gomez, I;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, K;Chien, K;Bueso-Ramos, C;Kantarjian, H;Garcia-Manero, G;Transcriptomic Features and Deregulation of Genes Involved in Monopoiesis Influence Outcomes of Patients with Chronic Myelomonocytic LeukemiaBloodINTRODUCTION: Chronic myelomonocytic leukemia (CMML) is characterized by TET2, SRSF2, ASXL1 and RAS pathway mutations known to induce myelomonocytic bias. We have previously shown that upregulation of KDM6B, a histone demethylase that acts as an innaFull Paper
2020Walters, M;Chui, D;Farrell, J;Lal, A;Locatelli, F;Kwiatkowski, J;Porter, J;Sauer, M;Thuret, I;Hongeng, S;Kulozik, A;Thrasher, A;Yannaki, E;Yang, J;Whitney, D;Petrusich, A;Colvin, R;Thompson, A;Response of Patients with Transfusion-Dependent β-Thalassemia (TDT) to Betibeglogene Autotemcel (beti-cel; LentiGlobin for β-Thalassemia) Gene Therapy Based on HBB Genotype and Disease Genetic ModifiersBloodIntroduction We investigated the impact of β-thalassemia genotypes and disease genetic modifiers including HBA and KLF1 genotype and sentinel single-nucleotide polymorphism (SNP) genotypes at 3 major HbF quantitative trait loci (QTL) oFull Paper
2020Kanter, J;Tisdale, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Chen, R;Gallagher, M;Ding, Y;Goyal, S;Paramore, C;Thompson, A;Walters, M;Improvements in Health-Related Quality of Life for Patients Treated with LentiGlobin for Sickle Cell Disease (bb1111) Gene TherapyBloodBackground In patients with sickle cell disease (SCD), health-related quality of life (HRQoL) is worse than in the general population and comparable or worse than in patients with other chronic or painful diseases such as cystic fibrosFull Paper
2020Thompson, A;Kwiatkowski, J;Porter, J;Hongeng, S;Yannaki, E;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Guo, R;Liu, W;Colvin, R;Walters, M;Locatelli, F;Favorable Outcomes in Pediatric Patients in the Phase 3 Hgb-207 (Northstar-2) and Hgb-212 (Northstar-3) Studies of Betibeglogene Autotemcel Gene Therapy for the Treatment of Transfusion-Dependent β-ThalassemiaBloodIntroduction Betibeglogene autotemcel (beti-cel; LentiGlobin for β-thalassemia) gene therapy is being evaluated for the treatment of transfusion-dependent β-thalassemia (TDT). Initial positive results of beti-cel in the phase 3 studiesFull Paper
2020Kwiatkowski, J;Walters, M;Hongeng, S;Locatelli, F;Rasko, J;Cavazzana, M;Chen, Y;Colvin, R;Thompson, A;Long-Term Efficacy and Safety of Betibeglogene Autotemcel Gene Therapy for the Treatment of Transfusion-Dependent β-Thalassemia: Results in Patients with up to 6 Years of Follow-upBloodIntroduction The goal of betibeglogene autotemcel (beti-cel; LentiGlobin for β-thalassemia) gene therapy in patients with transfusion-dependent β-thalassemia (TDT) is lifelong, stable production of functional adult hemoglobin (Hb) suffFull Paper
2020Thompson, A;Walters, M;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Schmidt, M;DelCarpini, J;Pierciey, F;Miller, A;Gallagher, M;Chen, R;Goyal, S;Kanter, J;Tisdale, J;Resolution of Serious Vaso-Occlusive Pain Crises and Reduction in Patient-Reported Pain Intensity: Results from the Ongoing Phase 1/2 HGB-206 Group C Study of LentiGlobin for Sickle Cell Disease (bb1111) Gene TherapyBloodBackground Sickle cell disease (SCD) is caused by abnormal sickle hemoglobin (HbS) and results in chronic hemolytic anemia, painful vaso-occlusive events (VOEs), and progressive vasculopathy that lead to significant morbidity. While acFull Paper
2020Rothe, K;Babaian, A;Nakamichi, N;Chen, M;Chafe, SC;Watanabe, A;Forrest, DL;Mager, DL;Eaves, CJ;Dedhar, S;Jiang, X;Integrin-Linked Kinase Mediates Therapeutic Resistance of Quiescent CML Stem Cells to Tyrosine Kinase InhibitorsCell stem cell32413332Full Paper
2020Nakao, S;Arai, Y;Tasaki, M;Yamashita, M;Murakami, R;Kawase, T;Amino, N;Nakatake, M;Kurosaki, H;Mori, M;Takeuchi, M;Nakamura, T;Intratumoral expression of IL-7 and IL-12 using an oncolytic virus increases systemic sensitivity to immune checkpoint blockadeScience translational medicine31941828hIL-7/hIL-12-VV at MOI 1.0 and cultured for 2 days. Then, the supernatant was filtered through 0.1-μm polyvinylidene difluoride membrane (Millipore) and added to 1 × 105 human PBMCs (AllCells) in a 96-well plate. Seven days later, secretion of humanFull Paper
2020Geng, J;Liu, W;Zhou, H;Zhang, T;Wang, L;Zhang, M;Li, Y;Shen, B;Li, X;Xiao, B;A Plug-and-Latch Mechanism for Gating the Mechanosensitive Piezo ChannelNeuron32142647HEK293T Xiao et al., 2011 N/A HEK293T-Piezo1-KO Cahalan et al., 2015 N/A HUVEC Allcells Cat# H-001F-C HeLa Xiao et al., 2011 N/A N2A Coste et al., 2010 N/A C2C12 National Infrastructure of Cell line Source, China Cat# GNM26Full Paper
2020Fang, C;Wang, Z;Han, C;Safgren, SL;Helmin, KA;Adelman, ER;Serafin, V;Basso, G;Eagen, KP;Gaspar-Maia, A;Figueroa, ME;Singer, BD;Ratan, A;Ntziachristos, P;Zang, C;Cancer-specific CTCF binding facilitates oncogenic transcriptional dysregulationGenome biology32933554Human CD4 + T cells were purchased from AllCells. Primary human samples were collected with informed consent and analyzed under the supervision of the Institutional Review Board SaveFull Paper
2020Li, M;Liu, W;Bauch, T;Graviss, EA;Arduino, RC;Kimata, JT;Chen, M;Wang, J;Clearance of HIV infection by selective elimination of host cells capable of producing HIVNature communications32792548pecific-pathogen-free barrier animal facility at the Houston Methodist Research Institute. Newborn male and female mice were injected intrahepatically with CD34+ human stem cells (5 × 104/mouse; AllCells LLC). Three months later, reconstitution of huFull Paper
2020Scharenberg, SG;Poletto, E;Lucot, KL;Colella, P;Sheikali, A;Montine, TJ;Porteus, MH;Gomez-Ospina, N;Engineering monocyte/macrophage-specific glucocerebrosidase expression in human hematopoietic stem cells using genome editingNature communications32620863Human CD34+ HSPCs mobilized from peripheral blood were purchased frozen from AllCells (Almeda, CA, USA) and thawed per manufacturer's instructions. Human Cord blood was SaveFull Paper
2020Wienert, B;Nguyen, DN;Guenther, A;Feng, SJ;Locke, MN;Wyman, SK;Shin, J;Kazane, KR;Gregory, GL;Carter, MAM;Wright, F;Conklin, BR;Marson, A;Richardson, CD;Corn, JE;Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repairNature communications32355159Cryopreserved wildtype human mobilized peripheral blood CD34 + HSPCs from volunteer donors were purchased from Allcells, and were cultured in SFEMII + CC110 (StemCell SaveFull Paper
2020Lareau, CA;Ma, S;Duarte, FM;Buenrostro, JD;Inference and effects of barcode multiplets in droplet-based single-cell assaysNature communications32054859lues have been published, these values were approximated from an examination of a plot previously reported2. Profiling PBMCs using 10× scATAC-seq For 10× scATAC-seq experiments with PBMCs (PB003F, Allcells), frozen cells were quickly thawed in a 37Full Paper
2020Mahuron, KM;Moreau, JM;Glasgow, JE;Boda, DP;Pauli, ML;Gouirand, V;Panjabi, L;Grewal, R;Luber, JM;Mathur, AN;Feldman, RM;Shifrut, E;Mehta, P;Lowe, MM;Alvarado, MD;Marson, A;Singer, M;Wells, J;Jupp, R;Daud, AI;Rosenblum, MD;Layilin augments integrin activation to promote antitumor immunityThe Journal of experimental medicine32539073Human peripheral blood mononuclear cells (PBMCs) from two individual donors were purchased from AllCells. CD8 + T cells were enriched from these samples using a negative SaveFull Paper
2020Poran, A;Scherer, J;Bushway, ME;Besada, R;Balogh, KN;Wanamaker, A;Williams, RG;Prabhakara, J;Ott, PA;Hu-Lieskovan, S;Khondker, ZS;Gaynor, RB;Rooney, MS;Srinivasan, L;Combined TCR Repertoire Profiles and Blood Cell Phenotypes Predict Melanoma Patient Response to Personalized Neoantigen Therapy plus Anti-PD-1Cell reports. Medicine33294862Patient PBMCs from NT-001 trial Ott et al., 2020 N/A Healthy Donor PBMCs Precision for Medicine Cat # 93000-10M Healthy Donor PBMCs StemExpress Cat # LE010F Healthy Donor PBMCs AllCells https://www.allcells.com/tissue-products/Full Paper
2020Huang, H;Zhu, H;Xie, Q;Tian, X;Yang, X;Feng, F;Jiang, Q;Sheng, X;Yang, Z;Evaluation of 124I-JS001 for hPD1 immuno-PET imaging using sarcoma cell homografts in humanized miceActa pharmaceutica Sinica. B32874831The human OS-732 cell line was purchased from the Institute of Cancer, Chinese Academy of Medical Sciences (Beijing, China). Normal peripheral blood T cells and CD3+ pan T cells were obtained from AllCells (PB009-1F-C, Emeryville, CA, USA). PhytohemaFull Paper
2020Poran, A;Harjanto, D;Malloy, M;Arieta, CM;Rothenberg, DA;Lenkala, D;van Buuren, MM;Addona, TA;Rooney, MS;Srinivasan, L;Gaynor, RB;Sequence-based prediction of SARS-CoV-2 vaccine targets using a mass spectrometry-based bioinformatics predictor identifies immunogenic T cell epitopesGenome medicine32791978Human PBMCs from HLA-A02:01-positive human donors were isolated using Ficoll separation from apheresis material (AllCells, USA). Twenty-three SARS-CoV-2 peptides predicted to SaveFull Paper
2020Goodwin, M;Lee, E;Lakshmanan, U;Shipp, S;Froessl, L;Barzaghi, F;Passerini, L;Narula, M;Sheikali, A;Lee, CM;Bao, G;Bauer, CS;Miller, HK;Garcia-Lloret, M;Butte, MJ;Bertaina, A;Shah, A;Pavel-Dinu, M;Hendel, A;Porteus, M;Roncarolo, MG;Bacchetta, R;CRISPR-based gene editing enables FOXP3 gene repair in IPEX patient cellsScience advances32494707families. Additional healthy donor HSPCs were isolated from umbilical cord blood donors provided by the Binns Program for Cord Blood Research at the Stanford University or purchased commercially from AllCells or StemCell Technologies. For HSPC isolatFull Paper
2020Grozdanovic, MM;Doyle, CB;Liu, L;Maybruck, BT;Kwatia, MA;Thiyagarajan, N;Acharya, KR;Ackerman, SJ;Charcot-Leyden crystal protein/galectin-10 interacts with cationic ribonucleases and is required for eosinophil granulogenesisThe Journal of allergy and clinical immunology31982451Purified human CD34 + cord blood-derived hematopoietic progenitor cells were purchased from AllCells (Emeryville, Calif) and cultured in Iscove modified Dulbecco medium SaveFull Paper
2020Lundin, V;Sugden, WW;Theodore, LN;Sousa, PM;Han, A;Chou, S;Wrighton, PJ;Cox, AG;Ingber, DE;Goessling, W;Daley, GQ;North, TE;YAP Regulates Hematopoietic Stem Cell Formation in Response to the Biomechanical Forces of Blood FlowDevelopmental cell32032546Cord Blood CD34+ Cells AllCells Cat. CB008F mPB CD34+ Cells AllCells Cat. mPB015F Mouse Embryonic Fibroblasts GlobalStem Cat. 6001G Primary Human Umbilical Vein Endothelial Cells ThermoFisher Cat. C0155CFull Paper
2020Duncan, RM;Reyes, L;Moats, K;Robinson, RM;Murphy, SA;Kaur, B;Stessman, HAF;Dolloff, NG;ATF3 Coordinates Antitumor Synergy between Epigenetic Drugs and Protein Disulfide Isomerase InhibitorsCancer research32561529T cells were expanded from human peripheral blood monoleukocytes purchased from AllCells LLC (PB006F). All cells were maintained at 37C and 5% CO 2 . Experiments were SaveFull Paper
2020Yeung, YA;Krishnamoorthy, V;Dettling, D;Sommer, C;Poulsen, K;Ni, I;Pham, A;Chen, W;Liao-Chan, S;Lindquist, K;Chin, SM;Chunyk, AG;Hu, W;Sasu, B;Chaparro-Riggers, J;Djuretic, I;An Optimized Full-Length FLT3/CD3 Bispecific Antibody Demonstrates Potent Anti-leukemia Activity and Reversible Hematological ToxicityMolecular therapy : the journal of the American Society of Gene Therapy31981494After tumor engraftment (day 6-7), animals were administered 2 × 10 7 expanded human T cells (T cells purchased from Allcells; activated according to Miltenyi manufacturing protocol) SaveFull Paper
2020Morgan, RA;Unti, MJ;Aleshe, B;Brown, D;Osborne, KS;Koziol, C;Ayoub, PG;Smith, OB;O'Brien, R;Tam, C;Miyahira, E;Ruiz, M;Quintos, JP;Senadheera, S;Hollis, RP;Kohn, DB;Improved Titer and Gene Transfer by Lentiviral Vectors Using Novel, Small β-Globin Locus Control Region ElementsMolecular therapy : the journal of the American Society of Gene Therapy31628051All BM aspirates were obtained from voluntary healthy donors supplied by AllCells (Alameda, CA), which obviated the need for Instituional Review Board review. BM mononuclear cells SaveFull Paper
2020Sinnakannu, JR;Lee, KL;Cheng, S;Li, J;Yu, M;Tan, SP;Ong, CCH;Li, H;Than, H;Anczuków-Camarda, O;Krainer, AR;Roca, X;Rozen, SG;Iqbal, J;Yang, H;Chuah, C;Ong, ST;SRSF1 mediates cytokine-induced impaired imatinib sensitivity in chronic myeloid leukemiaLeukemia32051529at the Singapore General Hospital, cord blood samples were obtained from the Singapore Cord Blood Bank, and normal bone marrow cells were obtained commercially from AllCells ( SaveFull Paper
2020Korotchkina, L;Kazyulkin, D;Komarov, PG;Polinsky, A;Andrianova, EL;Joshi, S;Gupta, M;Vujcic, S;Kononov, E;Toshkov, I;Tian, Y;Krasnov, P;Chernov, MV;Veith, J;Antoch, MP;Middlemiss, S;Somers, K;Lock, RB;Norris, MD;Henderson, MJ;Haber, M;Chernova, OB;Gudkov, AV;OT-82, a novel anticancer drug candidate that targets the strong dependence of hematological malignancies on NAD biosynthesisLeukemia31896781Normal human bone marrow mononuclear cells (BM-MNC) were obtained from ALLCELLS, LLC (Almeda CA, USA). Patients’ BM-MNC were procured at the Hematologic Bank of RPCCC and NAMPT monoclonal antibody (clone OMNI 379) was obtained from Cayman Chemicals (Full Paper
2020Kravtsova-Ivantsiv, Y;Goldhirsh, G;Ivantsiv, A;Ben Itzhak, O;Kwon, YT;Pikarsky, E;Ciechanover, A;Excess of the NF-ĸB p50 subunit generated by the ubiquitin ligase KPC1 suppresses tumors via PD-L1- and chemokines-mediated mechanismsProceedings of the National Academy of Sciences of the United States of America33168738Human PBMCs as a source for isolating human NK cells were from AllCells. Mouse anti-CD274 (PD-L1) (29E.2A3) and mouse anti-CD45 (HI30) for mass cytometry were from Bio SaveFull Paper
2020Ojaghi, A;Carrazana, G;Caruso, C;Abbas, A;Myers, DR;Lam, WA;Robles, FE;Label-free hematology analysis using deep-ultraviolet microscopyProceedings of the National Academy of Sciences of the United States of America32561645bone marrow aspirate was collected from healthy donors (AllCells) and added to Na heparin anticoagulant solution. Smears were made on uncoated quartz slides using 10 μL of whole SaveFull Paper
2020Huang, P;Zhao, Y;Zhong, J;Zhang, X;Liu, Q;Qiu, X;Chen, S;Yan, H;Hillyer, C;Mohandas, N;Pan, X;Xu, X;Putative regulators for the continuum of erythroid differentiation revealed by single-cell transcriptome of human BM and UCB cellsProceedings of the National Academy of Sciences of the United States of America32457162CD34 + stem/progenitor cells (AllCells) were obtained from three mobilized BM and three neonate UCB samples from independent donors. Cells were expanded at 37 C and 5% CO 2 SaveFull Paper
2020Belling, JN;Heidenreich, LK;Tian, Z;Mendoza, AM;Chiou, TT;Gong, Y;Chen, NY;Young, TD;Wattanatorn, N;Park, JH;Scarabelli, L;Chiang, N;Takahashi, J;Young, SG;Stieg, AZ;De Oliveira, S;Huang, TJ;Weiss, PS;Jonas, SJ;Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cellsProceedings of the National Academy of Sciences of the United States of America32358194Mixed donor umbilical cord CD34+ Hematopoietic stem and progenitor cells (Allcells Inc.) were thawed and prestimulated as described by Hoban et al. (44). Postacoustofluidic treatment, HSPCs were cultured in X-VIVO 15 supplemented with 50 ng/μL recombFull Paper
2020Borodovsky, A;Barbon, CM;Wang, Y;Ye, M;Prickett, L;Chandra, D;Shaw, J;Deng, N;Sachsenmeier, K;Clarke, JD;Linghu, B;Brown, GA;Brown, J;Congreve, M;Cheng, RK;Dore, AS;Hurrell, E;Shao, W;Woessner, R;Reimer, C;Drew, L;Fawell, S;Schuller, AG;Mele, DA;Small molecule AZD4635 inhibitor of A2AR signaling rescues immune cell function including CD103+ dendritic cells enhancing anti-tumor immunityJournal for immunotherapy of cancer3272781001 individuals. As previously described,38 naïve CD8+ T cells were co-cultured with autologous monocyte-derived DCs, both cell populations isolated from PBMC from HLA-A*0201 positive healthy donors (AllCells, Research Blood Components). Monocytes werFull Paper
2020Kotanides, H;Li, Y;Malabunga, M;Carpenito, C;Eastman, SW;Shen, Y;Wang, G;Inigo, I;Surguladze, D;Pennello, AL;Persaud, K;Hindi, S;Topper, M;Chen, X;Zhang, Y;Bulaon, DK;Bailey, T;Lao, Y;Han, B;Torgerson, S;Chin, D;Sonyi, A;Haidar, JN;Novosiadly, RD;Moxham, CM;Plowman, GD;Ludwig, DL;Kalos, M;Bispecific Targeting of PD-1 and PD-L1 Enhances T-cell Activation and Antitumor ImmunityCancer immunology research32873605Monocytes were isolated from frozen normal peripheral blood mononuclear cells (PBMC) purchased from AllCells (PB005F) using Monocyte Isolation Kit (Miltenyi Biotec; 130-091-153). Immature DCs were generated by culturing the monocytes in RPMI1640 mediFull Paper
2020Liu, Y;Lv, S;Liu, D;Song, F;Recent development of amorphous metal coordination polymers for cancer therapyActa biomaterialia32942012e) IC 50 values obtained from cytotoxicity assays against Jurkat human ALLcells after incubating with free MTX, Gd-MTX, [email protected]/DOPE, and [email protected]/DOPE-AA. f) SaveFull Paper
2020Fujita, H;Sasaki, T;Miyamoto, T;Akutsu, SN;Sato, S;Mori, T;Nakabayashi, K;Hata, K;Suzuki, H;Kosaki, K;Matsuura, S;Matsubara, Y;Amagai, M;Kubo, A;Premature aging syndrome showing random chromosome number instabilities with CDC20 mutationAging cell33094908An age-matched normal control bone marrow mononuclear cell sample was obtained from AllCells. Cells were cultured in MethoCult H4034 Optimum (STEMCELL Technologies) at 1 × SaveFull Paper
2020Yao, X;Ding, J;Effects of Microstripe Geometry on Guided Cell MigrationACS applied materials & interfaces32479054Primary rat mesenchymal stem cells (rMSCs) were purchased from Shanghai Allcells Tech. (China) and cultured in minimum essential medium α (MEM α, Gibco). Mouse embryo fibroblast cell line (NIH3T3) and human cervix epithelial carcinoma cell line (HelaFull Paper
2020Yang, H;Qing, K;Keeler, GD;Yin, L;Mietzsch, M;Ling, C;Hoffman, BE;Agbandje-McKenna, M;Tan, M;Wang, W;Srivastava, A;Enhanced Transduction of Human Hematopoietic Stem Cells by AAV6 Vectors: Implications in Gene Therapy and Genome EditingMolecular therapy. Nucleic acids32276210D, USA) supplemented with 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA) and 1% penicillin-streptomycin (Invitrogen, Grand Island, NY, USA). Human bone marrow CD34+ cells were purchased from AllCells (AllCells Technologies, Emeryville, CA, UFull Paper
2020Shin, JJ;Schröder, MS;Caiado, F;Wyman, SK;Bray, NL;Bordi, M;Dewitt, MA;Vu, JT;Kim, WT;Hockemeyer, D;Manz, MG;Corn, JE;Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor CellsCell reports32877675RNA-sequencing datasets This paper BioProject ID PRJNA498122 ------------------------- Experimental Models ------------------------- Mobilized peripheral blood CD34+ Stem/Progenitor cells AllCells mPB015F NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzFull Paper
2020Nie, W;Zan, X;Yu, T;Ran, M;Hong, Z;He, Y;Yang, T;Ju, Y;Gao, X;Synergetic therapy of glioma mediated by a dual delivery system loading α-mangostin and doxorubicin through cell cycle arrest and apoptotic pathwaysCell death & disease33116114animals The glioma cell lines C6, Gl261 and U87 and human umbilical vein endothelial cells (HUVECs) were purchased from the American Type Culture Collection (ATCC). Primary HUVECs were purchased from ALLCELLS (Shanghai, China). An EGM-2 BulletKit (LoFull Paper
2020Zhang, XY;Rajagopalan, D;Chung, TH;Hooi, L;Toh, TB;Tian, JS;Rashid, MBMA;Sahib, NRBM;Gu, M;Lim, JJ;Wang, W;Chng, WJ;Jha, S;Chow, EK;Frequent upregulation of G9a promotes RelB-dependent proliferation and survival in multiple myelomaExperimental hematology & oncology32477831e), 1% Pen Strep (Invitrogen). For adherent cell lines, trypsin (Thermo Scientific, cat# 25200-056) was used to dissociate cells each passage. Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs, AllCells) were cultured in RPMI 1640 medium (BioWeFull Paper
2020Qian, Z;Fei, J;Zong, S;Yang, K;Li, L;Liu, R;Wang, Z;Cui, Y;In Situ Visualization and SERS Monitoring of the Interaction between Tumor and Endothelial Cells Using 3D Microfluidic NetworksACS sensors31885254Human umbilical vein endothelial cells (HUVEC) were purchased from Shanghai AllCells Biotech. Co., Ltd. Breast cancer cells (MCF7) were purchased from Nanjing KeyGen Biotech. Co., Ltd. The Roswell Park Memorial Institute (RMPI) 1640 cell culture mediFull Paper
2020Kim, YH;Park, GY;Rabinovitch, N;Tarafder, S;Lee, CH;Effect of local anesthetics on viability and differentiation of various adult stem/progenitor cellsStem cell research & therapy32894184udy that directly compared the effects of LAs across various stem/progenitor cells. Materials and methods Cell isolation Human bone marrow mesenchymal stem/progenitor cells (MSCs) were obtained from AllCells (Alameda, CA). With the Institutional RevFull Paper
2020Xiong, MG;Xu, ZS;Li, YH;Wang, SY;Wang, YY;Ran, Y;RNF152 positively regulates TLR/IL-1R signaling by enhancing MyD88 oligomerizationEMBO reports31930677HEK293T cells were purchased from ATCC. Human PBMCs were purchased from ALLCELLS. HFFs provided by Prof. Min‐Hua Luo (Wuhan Institute of Virology, CAS) were derived from human foreskins (male) 30. These cells were cultured in DMEM (HyClone) supplemenFull Paper
2020Han, Y;Gong, T;Zhang, C;Dissanayaka, WL;HIF-1α Stabilization Enhances Angio-/Vasculogenic Properties of SHEDJournal of dental research32298193SHED were purchased from AllCells at passage 2 and characterized for mesenchymal origin. The results were published in our previous study (Xu et al. 2017). The cells were cultured in α-Modified Eagle’s Medium supplemented with 10% (v/v) fetal bovineFull Paper
2020Maser, IP;Hoves, S;Bayer, C;Heidkamp, G;Nimmerjahn, F;Eckmann, J;Ries, CH;The Tumor Milieu Promotes Functional Human Tumor-Resident Plasmacytoid Dendritic Cells in Humanized Mouse ModelsFrontiers in immunology33013879sulfan (Busilvex ,Pierre Faber) diluted in 0.9% Saline (Braun) was injected intraperitoneally (i.p.). Twenty-four hours later 105 human CD34+ umbilical cord blood-derived HSC (Stem Cell Technologies/Allcells) in 100 μl phosphate-buffered saline (PBS)Full Paper
2020Ghelani, A;Bates, D;Conner, K;Wu, MZ;Lu, J;Hu, YL;Li, CM;Chaudhry, A;Sohn, SJ;Defining the Threshold IL-2 Signal Required for Induction of Selective Treg Cell Responses Using Engineered IL-2 MuteinsFrontiers in immunology32582190CD8+ T cells were purified from PBMCs isolated from healthy human donor derived leukopaks (AllCells) using EasySep CD8+ T cell Negative selection kit (STEMCELL Technologies) SaveFull Paper
2020Shen, S;Sckisel, G;Sahoo, A;Lalani, A;Otter, DD;Pearson, J;DeVoss, J;Cheng, J;Casey, SC;Case, R;Yang, M;Low, R;Daris, M;Fan, B;Agrawal, NJ;Ali, K;Engineered IL-21 Cytokine Muteins Fused to Anti-PD-1 Antibodies Can Improve CD8+ T Cell Function and Anti-tumor ImmunityFrontiers in immunology32457754Mismatched donor pair leukopaks were obtained from AllCells Inc., Donor's T cells were isolated using Pan T-cell Isolation Kit (Miltenyi Biotec, # 130-096-535) and a mismatched donor' SaveFull Paper
2020Porcellini, S;Asperti, C;Corna, S;Cicoria, E;Valtolina, V;Stornaiuolo, A;Valentinis, B;Bordignon, C;Traversari, C;CAR T Cells Redirected to CD44v6 Control Tumor Growth in Lung and Ovary Adenocarcinoma Bearing MiceFrontiers in immunology32117253in of the ΔLNGFr (12). Generation of CD44v6 and CD19 CAR T Cells Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats or leukapheresis of healthy donors (San Raffaele Hospital; AllCells) by density gradient centrifugation (LymphFull Paper
2020Baslan, T;Kendall, J;Volyanskyy, K;McNamara, K;Cox, H;D'Italia, S;Ambrosio, F;Riggs, M;Rodgers, L;Leotta, A;Song, J;Mao, Y;Wu, J;Shah, R;Gularte-Mérida, R;Chadalavada, K;Nanjangud, G;Varadan, V;Gordon, A;Curtis, C;Krasnitz, A;Dimitrova, N;Harris, L;Wigler, M;Hicks, J;Novel insights into breast cancer copy number genetic heterogeneity revealed by single-cell genome sequencingeLife32401198ta (i.e. ploidy) to achieve absolute copy number values. T-cell and B-cell sample processing and analysis Purified CD8+/CD4+ T-cells and CD19+/CD27+ memory B-cells purified cells were purchased from ALLCELLS (California, USA). Cells were thawed on iFull Paper
2020Münchow, EA;da Silva, AF;Piva, E;Cuevas-Suárez, CE;de Albuquerque, MTP;Pinal, R;Gregory, RL;Breschi, L;Bottino, MC;Development of an antibacterial and anti-metalloproteinase dental adhesive for long-lasting resin composite restorationsJournal of materials chemistry. B33169763Human dental pulp stem cells (hDPSCs, AllCells, LLC, Alameda, CA, USA) obtained from permanent third molars were cultured in low glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Invitrogen Corporation, Grand Island, NY, USA) supplemented withFull Paper
2020Warnecke, A;Harre, J;Staecker, H;Prenzler, N;Strunk, D;Couillard-Despres, S;Romanelli, P;Hollerweger, J;Lassacher, T;Auer, D;Pachler, K;Wietzorrek, G;Köhl, U;Lenarz, T;Schallmoser, K;Laner-Plamberger, S;Falk, CS;Rohde, E;Gimona, M;Extracellular vesicles from human multipotent stromal cells protect against hearing loss after noise trauma in vivoClinical and translational medicine33377658Human bone marrow (BM)-derived MSCs for the production of research-grade EV preparations were purchased from AllCells (Alameda, CA). Immunophenotype and viability analysis of SaveFull Paper
2020Pinho, AC;Fonseca, AC;Caseiro, AR;Pedrosa, SS;Amorim, I;Branquinho, MV;Domingos, M;Maurício, AC;Santos, JD;Serra, AC;Coelho, JFJ;Innovative tailor made dextran based membranes with excellent non-inflammatory response: In vivo assessmentMaterials science & engineering. C, Materials for biological applications31761159Human dental pulp stem cells (hDPSCs) were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01). Minimum Essential Medium (MEM) α, GlutaMAX™ Supplement, no nucleosides, Streptomycin, Amphotericin B and HEPES buffer solution were purchaseFull Paper
2020Wang, J;Ganaie, SS;Cheng, F;Xu, P;Ning, K;Wang, X;Kleiboeker, S;Cheng, S;Qiu, J;RNA Binding Motif Protein RBM45 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19 through Binding to Novel Intron Splicing EnhancersmBio32156816se conditions are dependent on RBM45. MATERIALS AND METHODS Ethics statement. Primary CD34+ hematopoietic stem cells were isolated from bone marrow of healthy human donors. We purchased the cells at AllCells (AllCells LLC, Alameda, CA). All of the cFull Paper
2020Dubrovsky, L;Ward, A;Choi, SH;Pushkarsky, T;Brichacek, B;Vanpouille, C;Adzhubei, AA;Mukhamedova, N;Sviridov, D;Margolis, L;Jones, RB;Miller, YI;Bukrinsky, M;Inhibition of HIV Replication by Apolipoprotein A-I Binding Protein Targeting the Lipid RaftsmBio31964734PBMCs from donors with the following HLA-B genotypes were purchased from AllCells Inc.: B*35 positive (B*15:17:01/B*35:01:01, B*35:01:01/B*55:01:01, B*35:01:01/B*35:01:01, B*35: SaveFull Paper
2020Han, KH;Arlian, BM;Lin, CW;Jin, HY;Kang, GH;Lee, S;Lee, PC;Lerner, RA;Agonist Antibody Converts Stem Cells into Migrating Brown Adipocyte-Like Cells in HeartCells31968623one, Chicago, IL, USA) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA). Expi293F cells were cultured in Expi293 Expression Media (Invitrogen, Carlsbad, CA, USA). Human CD34+ cells (AllCells, Alameda, CA, USA) were purchased and repFull Paper
2020Zimran, E;Papa, L;Djedaini, M;Patel, A;Iancu-Rubin, C;Hoffman, R;Expansion and preservation of the functional activity of adult hematopoietic stem cells cultured ex vivo with a histone deacetylase inhibitorStem cells translational medicine31950644Cryopreserved CD34+ cells from mPB or ABM from healthy adult donors were purchased from AllCells (Alameda, California) and stored in liquid nitrogen. The highly purified (90%-98% SaveFull Paper
2020George, MJ;Prabhakara, K;Toledano-Furman, NE;Gill, BS;Wade, CE;Cotton, BA;Cap, AP;Olson, SD;Cox, CS;Procoagulant in vitro effects of clinical cellular therapeutics in a severely injured trauma populationStem cells translational medicine31903737Bone marrow mononuclear cells (BM MNCs) were isolated from fresh whole bone marrow from a commercial source (AllCells, Emeryville, California) according to common protocols SaveFull Paper
2020Hou, L;Voit, RA;Sankaran, VG;Springer, TA;Yuki, K;CD11c regulates hematopoietic stem and progenitor cells under stressBlood advances33351105Human bone marrow cells and whole blood were purchased from AllCells (Quincy, MA). Lineage marker for human hematopoietic system includes: anti-hCD2 (RPA-2.10), anti-hCD3 ( SaveFull Paper
2020Mascarenhas, JO;Rampal, RK;Kosiorek, HE;Bhave, R;Hexner, E;Wang, ES;Gerds, A;Abboud, CN;Kremyanskaya, M;Berenzon, D;Odenike, O;Farnoud, N;Krishnan, A;Weinberg, RS;McGovern, E;Salama, ME;Najfeld, V;Medina-Martinez, JS;Arango Ossa, JE;Levine, MF;Zhou, Y;Sandy, L;Heaney, ML;Levine, RL;Mesa, RA;Dueck, AC;Hoffman, R;Phase 2 study of ruxolitinib and decitabine in patients with myeloproliferative neoplasm in accelerated and blast phaseBlood advances33104796Six control plasma samples (3 male, 3 female) collected from healthy donors between the ages of 30 and 50 years were procured from Allcells. Samples from 8 chronic-phase MF SaveFull Paper
2020Marion, W;Boettcher, S;Ruiz-Torres, S;Lummertz da Rocha, E;Lundin, V;Morris, V;Chou, S;Zhao, AM;Kubaczka, C;Aumais, O;Zhang, Y;Shimamura, A;Schlaeger, TM;North, TE;Ebert, BL;Wells, SI;Daley, GQ;Rowe, RG;An induced pluripotent stem cell model of Fanconi anemia reveals mechanisms of p53-driven progenitor cell differentiationBlood advances33002135Institutional Review Board at Boston Children’s Hospital, after informed consent was obtained. This study was performed in accordance with the Declaration of Helsinki. Normal healthy BM mononuclear cells were purchased from AllCellsFull Paper
2020Goldstein, RL;Goyos, A;Li, CM;Deegen, P;Bogner, P;Sternjak, A;Thomas, O;Klinger, M;Wahl, J;Friedrich, M;Rattel, B;Lamas, E;Min, X;Sudom, A;Farshbaf, M;Coxon, A;Balazs, M;Arvedson, T;AMG 701 induces cytotoxicity of multiple myeloma cells and depletes plasma cells in cynomolgus monkeysBlood advances32886754For staining of human PCs, human BM (AllCells) was stained with a fluorochrome-labeled antibody cocktail (supplemental Table 5). For analysis, doublets were gated out, and dead SaveFull Paper
2020Walker, ZJ;VanWyngarden, MJ;Stevens, BM;Abbott, D;Hammes, A;Langouët-Astrie, C;Smith, CA;Palmer, BE;Forsberg, PA;Mark, TM;Jordan, CT;Sherbenou, DW;Measurement of ex vivo resistance to proteasome inhibitors, IMiDs, and daratumumab during multiple myeloma progressionBlood advances32311014Normal donor samples were purchased from AllCells. For viability comparison with MM cells in MNC cultures, selection for CD138-positive cells was performed by using magnetic bead SaveFull Paper
2020Romero-Masters, JC;Huebner, SM;Ohashi, M;Bristol, JA;Benner, BE;Barlow, EA;Turk, GL;Nelson, SE;Baiu, DC;Van Sciver, N;Ranheim, EA;Gumperz, J;Sherer, NM;Farrell, PJ;Johannsen, EC;Kenney, SC;B cells infected with Type 2 Epstein-Barr virus (EBV) have increased NFATc1/NFATc2 activity and enhanced lytic gene expression in comparison to Type 1 EBV infectionPLoS pathogens32059024ized NSG mice Immunodeficient NSG (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson labs (catalogue number: 005557). Commercially available CD34-depleted human cord blood mononuclear cells (AllCells, LLC) were infected with various amounts oFull Paper
2020Shao, S;Qin, T;Qian, W;Li, X;Li, W;Han, L;Zhang, D;Wang, Z;Ma, Q;Wu, Z;Wu, E;Lei, J;Cav-1 Ablation in Pancreatic Stellate Cells Promotes Pancreatic Cancer Growth through Nrf2-Induced shh SignalingOxidative medicine and cellular longevity32377291iated Hospital of Xi'an Jiaotong University, China. 2.3. Cell Lines Aspc-1 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and HUVECs were obtained from ALLCELLS (Emeryville, California, USA). In our expFull Paper
2020Liu, H;Cheng, Q;Xu, DS;Wang, W;Fang, Z;Xue, DD;Zheng, Y;Chang, AH;Lei, YJ;Overexpression of CXCR7 accelerates tumor growth and metastasis of lung cancer cellsRespiratory research33129326Human umbilical vein endothelial cells (HUVECs) and its completed medium were obtained from AllCells biological technology (Shanghai) co., LTD. And all these cell lines were SaveFull Paper
2020Cheng, B;Ren, Y;Cao, H;Chen, J;Discovery of novel resorcinol diphenyl ether-based PROTAC-like molecules as dual inhibitors and degraders of PD-L1European journal of medicinal chemistry32388281CD3 single chain variable fragment) and human PD-L1 (hPD-L1). Fresh PBMCs were purchased from ALLCELLS. Centrifuge at 350g for 10 min, discard the supernatant and resuspend in EasySep buffer at a density of 5 x107/mL. CD3+ T cells were isolated fromFull Paper
2020Zhang, D;Qi, BY;Zhu, WW;Huang, X;Wang, XZ;Crocin alleviates lipopolysaccharide-induced acute respiratory distress syndrome by protecting against glycocalyx damage and suppressing inflammatory signaling pathwaysInflammation research : official journal of the European Histamine Research Society ... [et al.]31925528HUVECs were cultured in complete culture medium (AllCells, Shanghai, China) in an incubator with a humidified atmosphere of 95% air and 5% CO 2 at 37 C. Cells at 80-90% SaveFull Paper
2020Kuang, Z;Jing, H;Wu, Z;Wang, J;Li, Y;Ni, H;Zhang, P;Wu, W;Wu, M;Zhou, S;Qiu, X;Wu, D;Prinz, B;Baruah, H;Chen, B;Yu, M;Liu, J;Development and characterization of a novel anti-OX40 antibody for potent immune activationCancer immunology, immunotherapy : CII32078015Two million LoVo cells suspended in 0.2 ml PBS were mixed with 0.66 million human peripheral blood mononuclear cells (PBMCs, ALLCELLS) and co-implanted subcutaneously in the SaveFull Paper
2020Yao, Y;Yang, J;Qin, Q;Tang, C;Li, Z;Chen, L;Li, K;Ren, C;Chen, L;Rao, S;Functional annotation of genetic associations by transcriptome-wide association analysis provides insights into neutrophil development regulationCommunications biology33340029Human CD34 + HSPCs from mobilized peripheral blood of deidentified healthy donors were purchased from AllCells. CD34 + HSPCs were cultured in StemSpan SFEM medium ( SaveFull Paper
2020Mendes, LP;Rostamizadeh, K;Gollomp, K;Myerson, JW;Marcos-Contreras, OA;Zamora, M;Luther, E;Brenner, JS;Filipczak, N;Li, X;Torchilin, VP;Monoclonal antibody 2C5 specifically targets neutrophil extracellular trapsmAbs33323006Whole blood was purchased from AllCells (AllCells utilizes standardized operating HIPAA compliance and approved protocols are followed by AllCells' collection facilities. The staff SaveFull Paper
2020Zang, L;Song, Y;Yu, F;Liu, X;Emodin relieved lipopolysaccharide-evoked inflammatory damage in WI-38 cells by up-regulating taurine up-regulated gene 1BioFactors (Oxford, England)31912578The human fibroblasts cell line WI-38 (AllCells, Shanghai, China) was hatched in a mixture of Dulbecco's modified Eagle's medium (DMEM; Gibco, Grand Island, NY), endothelial cell development supplement (Sigma, St. Louis, MO) (60 μg/ml), streptomycinFull Paper
2020Subhi, H;Husein, A;Mohamad, D;Nurul, A;Physicochemical, mechanical and cytotoxicity evaluation of chitosan-based accelerated portland cementJournal of Materials Research and TechnologyStem cells from human exfoliated deciduous teeth (SHED) was purchased from AllCells, USA. The cells were cultured in alpha minimum essential medium [Invitrogen, USA (GIBCO)] SaveFull Paper
2020Hu, S;Cavanagh, BL;Harrington, R;Ahmad, M;Kearns, G;Meaney, S;Wynne, C;A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus PatientsInternational journal of molecular sciences33287382Microparticles are sub-micron, membrane-bound particles released from virtually allcells and which are present in the circulation. In several autoimmune disorders their amountand SaveFull Paper
2020Wen, W;Song, S;Han, Y;Chen, H;Liu, X;Qian, Q;An efficient Screening System in Yeast to Select a Hyperactive piggyBac Transposase for Mammalian ApplicationsInternational journal of molecular sciences32357554fied eagle medium (DMEM; Corning, Corning, NY, USA) supplemented with 10% fetal calf serum (FBS, Gibco, USA). The human peripheral blood mononuclear cells (PBMC) of healthy donors were purchased from AllCells (AllCells, Silicon Valley, CA, USA) underFull Paper
2020Damodharan, SN;Walker, KL;Forsberg, MH;McDowell, KA;Bouchlaka, MN;Drier, DA;Sondel, PM;DeSantes, KB;Capitini, CM;Analysis of ex vivo expanded and activated clinical-grade human NK cells after cryopreservationCytotherapy32536506All cellular expansions occurred at the WBF under current Good Manufacturing Practice (GMP) quality system guidelines. Fresh leukopacks were commercially obtained (AllCells, Alameda, CA, USA, and Key Biologics, Memphis, TN, USA) from paid human apherFull Paper
2020Chen, Q;Jia, G;Zhao, X;Bao, Y;Zhang, Y;Ozkan, C;Minev, B;Ma, W;Novel Survivin Peptides Screened With Computer Algorithm Induce Cytotoxic T Lymphocytes With Higher Cytotoxic Efficiency to Cancer CellsFrontiers in molecular biosciences33102521PBMCs from five healthy donors were provided by San Diego Blood Bank and Scripps Green Hospital (San Diego, CA, United States). Normal human umbilical cord blood (UCB) CD34+ cells were purchased from AllCells (Alameda, CA, United States). This studyFull Paper
2020Papa, L;Djedaini, M;Martin, TC;Zangui, M;Beaumont, KG;Sebra, R;Parsons, R;Schaniel, C;Hoffman, R;Limited Mitochondrial Activity Coupled With Strong Expression of CD34, CD90 and EPCR Determines the Functional Fitness of ex vivo Expanded Human Hematopoietic Stem CellsFrontiers in cell and developmental biology33384995Cryopreserved CD34 + cells from G-CSF mobilized peripheral blood (PB) or bone marrow (BM) aspirates harvested from healthy adult donors were purchased from AllCells. SaveFull Paper
2020Yao, X;Xie, L;Zeng, Y;MiR-9 Promotes Angiogenesis via Targeting on Sphingosine-1- Phosphate Receptor 1Frontiers in cell and developmental biology32850858and S1P3 in those normal ECs and tumor ECs were compared. The relationships between the significant differentially expressed miR-9 loci and specific S1P receptors were analyzed. Cell Culture HUVECs (Allcells, Shanghai, China) were cultured in HUVECFull Paper
2020Juillerat, A;Tkach, D;Yang, M;Boyne, A;Valton, J;Poirot, L;Duchateau, P;Straightforward Generation of Ultrapure Off-the-Shelf Allogeneic CAR-T CellsFrontiers in bioengineering and biotechnology32671047ndependent T-cell donors. Significance is determined by a standard unpaired t-test, *p ≤ 0.05, **p ≤ 0.01. Materials and Methods T-Cell Proliferation Cryopreserved human PBMCs were acquired from ALLCELLS (cat #PB006F) and used in accordance with CelFull Paper
2020Kuang, Z;Pu, P;Wu, M;Wu, Z;Wang, L;Li, Y;Zhang, S;Jing, H;Wu, W;Chen, B;Liu, J;A Novel Bispecific Antibody with PD-L1-assisted OX40 Activation for Cancer TreatmentMolecular cancer therapeutics32999045Monocyte-derived immature dendrite cells (DC) isolated from human PBMCs (ALLCELLS) were maintained in AIM V medium CTS medium supplemented with GM-CSF (10 ng/mL, R&D SaveFull Paper
2020Kotanides, H;Sattler, RM;Lebron, MB;Carpenito, C;Shen, J;Li, J;Surguladze, D;Haidar, JN;Burns, C;Shen, L;Inigo, I;Pennello, AL;Forest, A;Chen, X;Chin, D;Sonyi, A;Topper, M;Boucher, L;Sharma, P;Zhang, Y;Burtrum, D;Novosiadly, RD;Ludwig, DL;Plowman, GD;Kalos, M;Characterization of 7A5: A Human CD137 (4-1BB) Receptor Binding Monoclonal Antibody with Differential Agonist Properties That Promotes Antitumor ImmunityMolecular cancer therapeutics32241872Human PBMCs were isolated from whole blood or leukopak obtained from NY Blood Center, AllCells, or Biological Specialty using Ficoll density gradient centrifugation (Ficoll-Paque SaveFull Paper
2020Bian, W;Jing, X;Yang, Z;Shi, Z;Chen, R;Xu, A;Wang, N;Jiang, J;Yang, C;Zhang, D;Li, L;Wang, H;Wang, J;Sun, Y;Zhang, C;Downregulation of LncRNA NORAD promotes Ox-LDL-induced vascular endothelial cell injury and atherosclerosisAging32267831(Shanghai, China). The C57BL/6 J mice fed with a normal diet were labeled as the WT control group. Cell culture, RNA interference and Adenovirus infection Primary HUVECs were obtained from Shanghai AllCells Biotech Co., Ltd. (Shanghai, China) and cuFull Paper
2020Karuna, R;Yokokawa, F;Wang, K;Zhang, J;Xu, H;Wang, G;Ding, M;Chan, WL;Abdul Ghafar, N;Leonardi, A;Seh, CC;Seah, PG;Liu, W;Srinivasa, RPS;Lim, SP;Lakshminarayana, SB;Growcott, E;Babu, S;Fenaux, M;Zhong, W;Gu, F;Shi, PY;Blasco, F;Chen, YL;A Cyclic Phosphoramidate Prodrug of 2'-Deoxy-2'-Fluoro-2'-C-Methylguanosine for the Treatment of Dengue Virus InfectionAntimicrobial agents and chemotherapy32958712PBMCs (individual donors) were purchased from AllCells (Alameda, CA) or ReachBio (Seattle, WA). Written consent from the donors was available for all samples. All experiments involving human matrices were approved by the Institutional Review Board ofFull Paper
2020Sebrow, J;Goff, SP;Griffin, DO;Successfully transfected primary peripherally mobilized human CD34+ hematopoietic stem and progenitor cells (HSPCs) demonstrate increased susceptibility to retroviral infectionVirology journal32039735We obtained 293 T cells and Jurkat cells from ATCC, and peripherally mobilized CD34+ HSPCs from individual donors from AllCells (pre-isolated). 293 T cells were cultured in 20 mm X SaveFull Paper
2020Zhong, C;Yu, Q;Jia, W;Yu, X;Yu, D;Yang, M;Wang, L;Ling, C;Zhu, L;Mechanism for enhanced transduction of hematopoietic cells by recombinant adeno-associated virus serotype 6 vectorsFASEB journal : official publication of the Federation of American Societies for Experimental Biology32960474Primary human cord blood-derived CD34 + hematopoietic progenitors (mixed, 80% purity) were purchased from AllCells (Alameda, CA, USA) and cultured in C-IMDM in the presence of 1 ng/mL of recombinant human stem cell factor (rhSCF, BD Bioscience, San DFull Paper
2020Li, L;Wu, Z;Wu, M;Qiu, X;Wu, Y;Kuang, Z;Wang, L;Sun, T;Liu, Y;Yi, S;Jing, H;Zhou, S;Chen, B;Wu, D;Wu, W;Liu, J;IBI112, a selective anti-IL23p19 monoclonal antibody, displays high efficacy in IL-23-induced psoriasiform dermatitisInternational immunopharmacology33069927Mouse (C57BL/6, Beijing Vital River Laboratory Animal Technology) or cynomolgus monkey splenocytes (AllCells, USA) were cultured in the presence of IL-2 (R&D, USA). Different SaveFull Paper
2020Kaneko, Y;Fukahori, H;Yamagami, K;Kawashima, T;Ito, M;Akamatsu, M;Marui, T;Kato, K;Takahashi, F;Morokata, T;Effects of AS2819899, a novel selective PI3Kδ inhibitor, in a NZB/W F1 mouse lupus-like nephritis modelInternational immunopharmacology32736191Peripheral blood-derived frozen normal pan-T cells (AllCells, Alameda, CA, USA) were thawed according to the manufacturer’s instructions. The cell suspension (1 × 105 cells/well) was mixed with AS2819899 at designated concentrations in the anti-CD3 aFull Paper
2020Goydel, RS;Weber, J;Peng, H;Qi, J;Soden, J;Freeth, J;Park, H;Rader, C;Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applicationsThe Journal of biological chemistry32193207Thr-245 and Ala-245, which arise from an SNP (rs10820900) in the frizzled domain of human ROR2 (hROR2-Fz). Human PBMCs were purchased from AllCells and cultured in X-VIVO 20 medium (Lonza) with 5% (v/v) off-the-clot human AB serum (Gemini Bio-ProductFull Paper
2020Čuperlović-Culf, M;Khieu, NH;Surendra, A;Hewitt, M;Charlebois, C;Sandhu, JK;Analysis and Simulation of Glioblastoma Cell Lines-Derived Extracellular Vesicles MetabolomeMetabolites32131411Normal human astrocytes (NHA, Lonza, Walkersville, MD, USA) were used as normal counterparts and were grown in astrocyte medium-containing supplements (Allcells) and 5% FBS. SaveFull Paper
2020Liu, J;Kozhaya, L;Torres, VJ;Unutmaz, D;Lu, M;Structure-based discovery of a small-molecule inhibitor of methicillin-resistant Staphylococcus aureus virulenceThe Journal of biological chemistry32179646Blood samples were obtained from healthy, consenting donors as buffy coats (New York Blood Center) and leukopaks (AllCells, Alameda, CA). Human PBMCs were isolated from peripheral blood by density gradient centrifugation using Ficoll-Paque Plus (GE LFull Paper
2020Thwin, KKM;Ishida, T;Uemura, S;Yamamoto, N;Lin, KS;Tamura, A;Kozaki, A;Saito, A;Kishimoto, K;Mori, T;Hasegawa, D;Kosaka, Y;Nino, N;Takafuji, S;Iijima, K;Nishimura, N;Level of Seven Neuroblastoma-Associated mRNAs Detected by Droplet Digital PCR Is Associated with Tumor Relapse/Regrowth of High-Risk Neuroblastoma PatientsThe Journal of molecular diagnostics : JMD318374271 sample from BioChain (Newark, CA), 2 samples from STEMCELL (Vancouver, Canada), 56 samples from AllCells (Alameda, CA), 16 samples from ReachBio (Seattle, WA), and 28 samples from Loza (Walkersville, MD) (Supplemental Table S1). All PB samples fromFull Paper
2020Xue, WL;Chen, RQ;Zhang, QQ;Li, XH;Cao, L;Li, MY;Li, Y;Lin, G;Chen, Y;Wang, MJ;Zhu, YC;Hydrogen sulfide rescues high glucose-induced migration dysfunction in HUVECs by upregulating miR-126-3pAmerican journal of physiology. Cell physiology32186933Primary human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) (35). These HUVECs were cultured in endothelial complete medium (Allcells, SaveFull Paper
2020Satani, N;Giridhar, K;Cai, C;Wewior, N;Norris, DD;Aronowski, J;Savitz, SI;Medications for Hypertension Change the Secretome Profile from Marrow Stromal Cells and Peripheral Blood MonocytesStem cells international32802081MSCs were isolated from commercially available fresh human bone marrow aspirates (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously SaveFull Paper
2020Diaz, MF;Horton, PD;Dumbali, SP;Kumar, A;Livingston, M;Skibber, MA;Mohammadalipour, A;Gill, BS;Zhang, S;Cox, CS;Wenzel, PL;Bone marrow stromal cell therapy improves survival after radiation injury but does not restore endogenous hematopoiesisScientific reports33335275t received wet feed on days 4-30 post-exposure. Culture of human MSCs Human MSCs used for cell therapy were derived from whole bone marrow from independent human donors (commercially obtained from AllCells, LLC, Alameda, CA). Cells were isolated andFull Paper
2020Dzimianski, JV;Lorig-Roach, N;O'Rourke, SM;Alexander, DL;Kimmey, JM;DuBois, RM;Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometryScientific reports33303951dy at UCSC. All participants agreed to sample banking and future research use. The convalescent seropositive (SP) panel comprised 10 de-identified plasma samples from nine individuals, purchased from AllCells (Alameda, CA, USA). To be eligible for plFull Paper
2020Diaz, MF;Horton, PD;Kumar, A;Livingston, M;Mohammadalipour, A;Xue, H;Skibber, MA;Ewere, A;Toledano Furman, NE;Aroom, KR;Zhang, S;Gill, BS;Cox, CS;Wenzel, PL;Injury intensifies T cell mediated graft-versus-host disease in a humanized model of traumatic brain injuryScientific reports32612177Bone marrow stromal cells were derived from whole bone marrow from independent human donors (AllCells, Alameda, CA). Mononuclear cells from whole bone marrow were enriched SaveFull Paper
2020Allen, A;Vaninov, N;Li, M;Nguyen, S;Singh, M;Igo, P;Tilles, AW;O'Rourke, B;Miller, BLK;Parekkadan, B;Barcia, RN;Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune CellsScientific reports32576889ld times cover the range of expected delivery of the device from cell seeding location to clinical application. PBMC isolation and enrichment Peripheral blood mononuclear cells (PBMCs) obtained from AllCells LLC (AllCells, CA, USA) were freshly isolFull Paper
2020Siew Ching, H;Thirumulu Ponnuraj, K;Luddin, N;Ab Rahman, I;Nik Abdul Ghani, NR;Early Odontogenic Differentiation of Dental Pulp Stem Cells Treated with Nanohydroxyapatite-Silica-Glass Ionomer CementPolymers32957636the present study was principally aimed at evaluating the effect of nanoHA-silica-GIC on the differentiation of DPSCs into odontogenic lineage. 2. Materials and Methods 2.1. Cell Culture DPSCs (AllCells, Alameda, CA, USA; Cat no. DP003F) were grownFull Paper
2020Keskar, V;Sood, A;Loghin, E;Kovacs, E;Duthie, RS;Liu, S;Park, JH;Chadwick, C;Smith, R;Brown, M;Stroncek, DF;Highfill, SL;Novel DNA-based T-Cell Activator Promotes Rapid T-Cell Activation and ExpansionJournal of immunotherapy (Hagerstown, Md. : 1997)32796275Activation studies were conducted in 6-well tissue culture plastic plates using commercially available Pan T cells (AllCells, Alameda, CA). Cultures were diluted as needed (1:3 or 1:4) to SaveFull Paper
2020Shrestha, B;Zhang, Y;Yu, B;Li, G;Boucher, JC;Beatty, NJ;Tsai, HC;Wang, X;Mishra, A;Sweet, K;Lancet, JE;Kelley, L;Davila, ML;Generation of Antitumor T Cells For Adoptive Cell Therapy With Artificial Antigen Presenting CellsJournal of immunotherapy (Hagerstown, Md. : 1997)31834208To investigate the ability of aAPCs to support T-cell expansion we isolated T cells from healthy donor PBMCs (ALLcells) using a Human T-cell isolation Kit (Stem Cells Inc., Cambridge, SaveFull Paper
2020Park, LM;Lannigan, J;Jaimes, MC;OMIP-069: Forty-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral BloodCytometry. Part A : the journal of the International Society for Analytical Cytology32830910All human PBMCs used in this study were obtained from AllCells Alameda. Ethical review and regulatory compliance were conducted by Alpha Independent Review Board under SaveFull Paper
2020Nair, RR;Piktel, D;Hathaway, QA;Rellick, SL;Thomas, P;Saralkar, P;Martin, KH;Geldenhuys, WJ;Hollander, JM;Gibson, LF;Pyrvinium Pamoate Use in a B cell Acute Lymphoblastic Leukemia Model of the Bone Tumor MicroenvironmentPharmaceutical research31989336Primary CD3+ T cells, peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) were purchased from AllCells (Allcells.com) and maintained in Lymphocyte Growth Medium-3 (Lonza, Cat No: CC-3211) containing 10% FBS and 1x strepFull Paper
2020Powell, AB;Ren, Y;Korom, M;Saunders, D;Hanley, PJ;Goldstein, H;Nixon, DF;Bollard, CM;Lynch, RM;Jones, RB;Cruz, CRY;Engineered Antigen-Specific T Cells Secreting Broadly Neutralizing Antibodies: Combining Innate and Adaptive Immune Response against HIVMolecular therapy. Methods & clinical development33005704Peripheral blood samples were obtained from deidentified, healthy donor buffy coats from the NIH, through the Department of Transfusion Medicine, or commercially from AllCells. Save;erved. Peripheral Blood Samples Peripheral blood samples were obtFull Paper
2020Fang, Y;Zhang, Y;Guo, C;Chen, C;Gao, H;Zhou, X;Liu, T;Qian, Q;Safety and Efficacy of an Immune Cell-Specific Chimeric Promoter in Regulating Anti-PD-1 Antibody Expression in CAR T CellsMolecular therapy. Methods & clinical development32995356PBMCs were purchased from AllCells (USA) using a protocol approved by the Ethics Committee of the Chinese Peoples' Liberation Army General Hospital (S2019-063-01). In total, 5 × Save;Cell Isolation, Electroporation, and Ex Vivo CAR T Cell ExpansionFull Paper
2020Shapiro, J;Iancu, O;Jacobi, AM;McNeill, MS;Turk, R;Rettig, GR;Amit, I;Tovin-Recht, A;Yakhini, Z;Behlke, MA;Hendel, A;Increasing CRISPR Efficiency and Measuring Its Specificity in HSPCs Using a Clinically Relevant SystemMolecular therapy. Methods & clinical development32478125(5 μL total volume). Complexes were allowed to form for 10-20 min at room temperature before electroporation. Genome Editing by RNP Electroporation in CD34+ HSPCs Mobilized human CD34+ HSPCs (AllCells, Alameda, CA, USA) were thawed and cultured forFull Paper
2020Morgan, RA;Ma, F;Unti, MJ;Brown, D;Ayoub, PG;Tam, C;Lathrop, L;Aleshe, B;Kurita, R;Nakamura, Y;Senadheera, S;Wong, RL;Hollis, RP;Pellegrini, M;Kohn, DB;Creating New β-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer SequencesMolecular therapy. Methods & clinical development32426415BM CD34+ Cell Culture and Transduction All BM aspirates were obtained from voluntary healthy donors supplied by AllCells (Alameda, CA, USA). BM mononuclear cells were isolated SaveFull Paper
2020Mehler, VJ;Burns, CJ;Stauss, H;Francis, RJ;Moore, ML;Human iPSC-Derived Neural Crest Stem Cells Exhibit Low ImmunogenicityMolecular therapy. Methods & clinical development32055644utamine, 2 mM L-glutamine, 50 U/mL penicillin/streptomycin, 20 ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF), and 20 ng/mL IL-4. BM-MSCs were derived from bone marrow aspirates (AllCells, Alameda, CA, USA) in-house as previously desFull Paper
2020Elnaggar, M;Al-Mohannadi, A;Kizhakayil, D;Raynaud, CM;Al-Mannai, S;Gentilcore, G;Pavlovski, I;Sathappan, A;Van Panhuys, N;Borsotti, C;Follenzi, A;Grivel, JC;Deola, S;Flow-Cytometry Platform for Intracellular Detection of FVIII in Blood Cells: A New Tool to Assess Gene Therapy Efficiency for Hemophilia AMolecular therapy. Methods & clinical development31886317ar endothelial cells) and U937 (pre-monocytic cells) cell lines were kindly provided by Antonia Follenzi (UPO, Italy). HeLa (cervical cancer cells) were purchased from ATCC. PBMCs were purchased from AllCells (Alameda, CA, USA). Cells were cultured iFull Paper
2020Ning, K;Wang, MJ;Lin, G;Zhang, YL;Li, MY;Yang, BF;Chen, Y;Huang, Y;Li, ZM;Huang, YJ;Zhu, L;Liang, K;Yu, B;Zhu, YZ;Zhu, YC;eNOS-Nitric Oxide System Contributes to a Novel Antiatherogenic Effect of Leonurine via Inflammation Inhibition and Plaque StabilizationThe Journal of pharmacology and experimental therapeutics32238453Primary human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (H-001F-C; China) and cultured in HUVEC Growth Medium (H-004; Allcells), and 100 U/ml of SaveFull Paper
2020Zhaorigetu, S;Bair, H;Jin, D;Gupta, VS;Pandit, LM;Bryan, RM;Lally, KP;Olson, SD;Cox, CS;Harting, MT;Extracellular Vesicles Attenuate Nitrofen-Mediated Human Pulmonary Artery Endothelial Dysfunction: Implications for Congenital Diaphragmatic HerniaStem cells and development32475301Human MSCs were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using density centrifugation and plastic SaveFull Paper
2020Baumgartner, JM;Riviere, P;Lanman, RB;Kelly, KJ;Veerapong, J;Lowy, AM;Kurzrock, R;Prognostic Utility of Pre- and Postoperative Circulating Tumor DNA Liquid Biopsies in Patients with Peritoneal MetastasesAnnals of surgical oncology32767050Background Circulating tumor DNA (ctDNA) is a promising technology for treatment selection, prognostication, and surveillance after definitive therapy. Its use in the perioperative SaveFull Paper
2020Hamburger, AE;DiAndreth, B;Cui, J;Daris, ME;Munguia, ML;Deshmukh, K;Mock, JY;Asuelime, GE;Lim, ED;Kreke, MR;Tokatlian, T;Kamb, A;Engineered T cells directed at tumors with defined allelic lossMolecular immunology33012527Leukopaks were purchased from AllCells®. Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB), with strict oversight. HIPAA compliance and approved protocols were also followed. Frozen PBMCs were thawedFull Paper
2020Xu, H;Hamburger, AE;Mock, JY;Wang, X;Martin, AD;Tokatlian, T;Oh, J;Daris, ME;Negri, KR;Gabrelow, GB;Wu, ML;Nampe, DP;Asuelime, GE;McElvain, ME;Sandberg, ML;Kamb, A;Structure-function relationships of chimeric antigen receptors in acute T cell responses to antigenMolecular immunology32768859Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols SaveFull Paper
2020Wang, Z;Zhou, G;Risu, N;Fu, J;Zou, Y;Tang, J;Li, L;Liu, H;Liu, Q;Zhu, X;Lenalidomide Enhances CAR-T Cell Activity Against Solid Tumor CellsCell transplantation32967454line U251 CD133-OE luc. Similarly, the tumor cell line U251 WT luc, MDA-MB-453 luc, and MDA-MB-468 luc were also obtained by nucleofection. Peripheral blood mononuclear cells (PBMCs) were bought from ALLCELLS (PB005F, Alameda, CA, USA). Following theFull Paper
2020Zhao, C;Zhao, Z;Wang, Z;Hu, L;Wang, H;Fang, Z;Supervillin promotes tumor angiogenesis in liver cancerOncology reports32468064Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells LLC (cat. H-004; AllCells LLC) in a 0.25% gelatin-coated culture flask. HepG2, Bel7405 and MHCC- SaveFull Paper
2020Lee, KS;Lee, J;Lee, P;Kim, CU;Kim, DJ;Jeong, YJ;Park, YJ;Tesh, VL;Lee, MS;Exosomes released from Shiga toxin 2a-treated human macrophages modulate inflammatory responses and induce cell death in toxin receptor expressing human cellsCellular microbiology32772454Human CD14 + monocytes were purchased from AllCells LLC and maintained in RPMI 1640 medium containing 10% FBS, 5.0 μg/ml streptomycin and 5.0 U/ml penicillin. Cells (1.0 × SaveFull Paper
2020Monroe, MN;Zhaorigetu, S;Gupta, VS;Jin, D;Givan, KD;Curylo, AL;Olson, SD;Cox, CS;Segura, A;Buja, LM;Grande-Allen, KJ;Harting, MT;Extracellular vesicles influence the pulmonary arterial extracellular matrix in congenital diaphragmatic herniaPediatric pulmonology32568428Human mesenchymal stromal cells (hMSCs) were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using SaveFull Paper
2020Zaw, SYM;Kaneko, T;Zaw, ZCT;Sone, PP;Murano, H;Gu, B;Okada, Y;Han, P;Katsube, KI;Okiji, T;Crosstalk between dental pulp stem cells and endothelial cells augments angiogenic factor expressionOral diseases32248596Stem cells from human exfoliated deciduous teeth (SHEDs; AllCells) were cultured with a stem cell growth medium (Dulbecco's modified Eagle's medium (DMEM)/F12; Gibco) containing 15% fetal bovine serum (Gibco-BRL, Grand Island, NY), 10,000 units penicFull Paper
2020Huang, GL;Nampe, DP;Yi, J;Gabrelow, GB;Negri, KR;Kamb, A;Xu, H;A multivariate, quantitative assay that disentangles key kinetic parameters of primary human T cell function in vitroPloS one33166305Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols SaveFull Paper
2020Wang, X;Chacon, LI;Derakhshandeh, R;Rodriguez, HJ;Han, DD;Kostyushev, DS;Henry, TD;Traverse, JH;Moyé, L;Simari, RD;Taylor, DA;Springer, ML;Impaired therapeutic efficacy of bone marrow cells from post-myocardial infarction patients in the TIME and LateTIME clinical trialsPloS one32841277Healthy de-identified donor BM MNCs were purchased from AllCells (Alameda, CA). These MNCs were manually isolated at AllCells using Ficoll conditions like that in the Sepax SaveFull Paper
2020Schönherz, AA;Bødker, JS;Schmitz, A;Brøndum, RF;Jakobsen, LH;Roug, AS;Severinsen, MT;El-Galaly, TC;Jensen, P;Johnsen, HE;Bøgsted, M;Dybkær, K;Normal myeloid progenitor cell subset-associated gene signatures for acute myeloid leukaemia subtyping with prognostic impactPloS one32324791[18] GSE42519, [19] GSE19599, [20] GSE17054, [21] and GSE19429 [22] cohorts. For GSE63270, [18] and GSE17054, [21] human bone marrow mononuclear cells (BMMCs) from healthy donors were purchased from ALLCELLS (Emeryville, CA) where collection protocolFull Paper
2020Tani, J;Ito, Y;Tatemichi, S;Yamakami, M;Fukui, T;Hatano, Y;Kakimoto, S;Kotani, A;Sugimura, A;Mihara, K;Yamamoto, R;Tanaka, N;Minami, K;Takahashi, K;Hirato, T;Physicochemical and biological evaluation of JR-131 as a biosimilar to a long-acting erythropoiesis-stimulating agent darbepoetin alfaPloS one32302352fects of the test substances on colony-forming unit-erythroid (CFU-E)- and burst-forming unit-erythroid (BFU-E)-derived colony formation in human bone-marrow-derived mononuclear cells (purchased from AllCells, CA) were examined. The cells were obtainFull Paper
2020Steinberg, L;The Effects of P. Gingivalis Ceramide Lipids on Platelet Size, Agglutination, Aggregation, and Vascular Surface Adhesion MarkersThesisBackground: Increased bleeding on probing has been proven to be correlated with the progression of periodontal disease. Gingival bleeding in sites with periodontitis has often been attributed to chronic inflammation. Previous research has documentedFull Paper
2020Kim, DH;Kim, DH;Heck, BE;Shaffer, M;Yoo, KH;Hur, J;PPAR-δ agonist affects adipo-chondrogenic differentiation of human mesenchymal stem cells through the expression of PPAR-γRegenerative therapy33426208In this experimental study, frozen human bone marrow mononuclear cells were used for isolation (AllCells, Alameda, CA, USA) [25]. Cells were resuspended in α-minimum essential Save;mation condition and its correlation with PPAR-δ. 2 Methods 2.1 IsFull Paper
2020Zhang, E;Xie, L;Qin, P;Lu, L;Xu, Y;Gao, W;Wang, L;Xie, MH;Jiang, W;Liu, S;Quality by Design-Based Assessment for Analytical Similarity of Adalimumab Biosimilar HLX03 to Humira®The AAPS journal32385732PMBC cells (Allcells) were incubated with mAbs with a 2-fold serial dilution for 30 min followed by TNFα-Biotin (Sinobio) incubation. After labeled by Streptavidin-PE (eBioscience), SaveFull Paper
2020Dai, R;Heid, B;Xu, X;Xie, H;Reilly, CM;Ahmed, SA;EGR2 is elevated and positively regulates inflammatory IFNγ production in lupus CD4+ T cellsBMC immunology32646370ethods Human peripheral blood mononuclear cells (PBMCs) The PBMCs of human patients of lupus (n = 4, all female) and healthy controls (n = 4, 2 male and 2 female) were purchased directly from AllCells LLC (Alameda, CA, USA). Based on the informationFull Paper
2020Dong, W;Zhang, X;Lu, Z;Effect of 532nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor in cultured human vascular endothelial cellsPhotodiagnosis and photodynamic therapy32344194Working drug solutions (0-8 μg/ml) were prepared by diluting the stock solution with RPMI-1640 medium (AllCells). A 532 nm neodymium-doped yttrium orthovanadate (Nd:YVO 4 ) SaveFull Paper
2020Pieles, O;Reck, A;Reichert, TE;Morsczeck, C;p53 inhibits the osteogenic differentiation but does not induce senescence in human dental follicle cellsDifferentiation; research in biological diversity32473528Human dental follicle cells (DFCs) were obtained from AllCells. DFCs were cultivated as described previously (Morsczeck et al., 2016). Cells were analyzed before and after the SaveFull Paper
2020Lafleur, MA;Werner, J;Fort, M;Lobenhofer, EK;Balazs, M;Goyos, A;MRGPRX2 activation as a rapid, high-throughput mechanistic-based approach for detecting peptide-mediated human mast cell degranulation liabilitiesJournal of immunotoxicology32525431Human mobilized peripheral blood CD34+ cells (derived from healthy donors treated with G-CSF to mobilize CD34+ progenitor cells from the bone marrow to the peripheral blood, then isolated using anti-CD34 magnetic beads) (#mPB016F, AllCells, Alameda,Full Paper
2020Yu, Z;Yin, S;Zhang, W;Jiang, X;Hu, J;Picosecond laser texturing on titanium alloy for biomedical implants in cell proliferation and vascularizationJournal of biomedical materials research. Part B, Applied biomaterials31692202Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCells Biological Technology Limited company and cultured at 1 × 105 on plates with Dulbecco's Modified Eagle's medium supplemented with 10% fetal bovine serum, 100 U/ml streptomycFull Paper
2020Bhargav, A;Min, KS;Wen Feng, L;Fuh, JYH;Rosa, V;Taguchi's methods to optimize the properties and bioactivity of 3D printed polycaprolactone/mineral trioxide aggregate scaffold: Theoretical predictions and experimental validationJournal of biomedical materials research. Part B, Applied biomaterials31112004The use of human dental pulp stem cells (DPSC) from single donor (DPF003, Allcells, Alameda) was approved by NUS Institutional Review Board (Approval Number: NUS 2094). The characterization and expressions of CD34, CD73, CD90, CD105 are available elsFull Paper
2020Al-Amoodi, AS;Sakashita, K;Ali, AJ;Zhou, R;Lee, JM;Tehseen, M;Li, M;Belmonte, JCI;Kusakabe, T;Merzaban, JS;Using Eukaryotic Expression Systems to Generate Human α1,3-Fucosyltransferases That Effectively Create Selectin-Binding Glycans on Stem CellsBiochemistry32901486leukemia cell line), THP1 (human acute monocytic leukemia), HL60 (human acute promyelocytic leukemia) (ATCC), and MSCs (primary mesenchymal stromal cells) (AllCells) were SaveFull Paper
2020Behbehani, GK;Finck, R;Samusik, N;Sridhar, K;Fantl, WJ;Greenberg, PL;Nolan, GP;Profiling myelodysplastic syndromes by mass cytometry demonstrates abnormal progenitor cell phenotype and differentiationCytometry. Part B, Clinical cytometry31917512in Table S2, with the risk‐based clinical status of MDS patients indicated by IPSS category and morphologic characteristics (Greenberg et al., 1997). Five healthy control samples were obtained from Allcells, Inc. using the same protocol. Normal boneFull Paper
2020Kim, DH;Kim, DH;Heck, BE;Shaffer, M;Hur, J;Yoo, KH;A natural supplement formula reduces anti-oxidative stress and enhances osteo-chondrogenic differentiation potential in mesenchymal stem cellsJournal of clinical biochemistry and nutrition32523247rozen mononuclear cells from human bone marrow were purchased (AllCells, Emeryville, CA) and used to isolate human bone marrow-mesenchymal stem cells (hBM-MSCs). Briefly, thawed mononuclear cells were cultured in Dulbecco’s modified Eagle medium (DMEFull Paper
2020Wang, P;Qin, W;Liu, T;Jiang, D;Cui, L;Liu, X;Fang, Y;Tang, X;Jin, H;Qian, Q;PiggyBac-engineered T cells expressing a glypican-3-specific chimeric antigen receptor show potent activities against hepatocellular carcinomaImmunobiology31522780Human peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from AllCells (AllCells, Silicon, USA). 5 × 106−1 × 107 PBMCs were electroporated with 6 μg of GPC3CAR plasmid or an equal quantity of MOCK plasmid in an electroporatFull Paper
2020Liu, H;Gemmell, L;Lin, R;Zuo, F;Balfour, HH;Woo, JC;Hayes, GM;Development of an Improved Epstein-Barr Virus (EBV) Neutralizing Antibody Assay to Facilitate Development of a Prophylactic gp350-Subunit EBV VaccineMediterranean journal of hematology and infectious diseases32180911Healthy human donor serum samples were purchased from AllCells, LLC and Bioreclamation, LLC (n=39). Samples were heated at 56 oC for 30 minutes to inactivate complement, and SaveFull Paper
2020Chien, KS;Class, CA;Montalban-Bravo, G;Wei, Y;Sasaki, K;Naqvi, K;Ganan-Gomez, I;Yang, H;Soltysiak, KA;Kanagal-Shamanna, R;Do, KA;Kantarjian, HM;Garcia-Manero, G;LILRB4 expression in chronic myelomonocytic leukemia and myelodysplastic syndrome based on response to hypomethylating agentsLeukemia & lymphoma32036728BM samples from healthy individuals were obtained from AllCells (Emeryville, CA, USA). CD34+ cells were isolated using the CD34 MicroBead Kit (Miltenyi Biotec, Bergisch Gladback, SaveFull Paper
2020Wu, T;The mean ± 1 SD was calculated for triplicate cultures. The laboratory (ReachBio LLC, Seattle, Washington) was blinded to the identify of the test compounds and docetaxel served as the positive control, with blank and vehicle as negative controls.ThesisThe surviving mice of the “young adult” group at day 35 post-TBI were sacrificed and 5×104 BM cells acquired from femurs were plated in ColonyGEL Mouse Complete Medium (Reach Bio, Seattle, WA) as previously described [131, 135]. After 7 days of incubFull Paper
2020White, CM;Haidekker, MA;Kisaalita, WS;Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D CulturesSLAS technology31997709Human bone marrow derived (Tulane Center for Gene Therapy) 3.2 ± 1.4 AFM indentation Spread morphology on glass coverslips; cytoplasm measured; spherical AFM tip 76 Human bone marrow derived (AllCells) 33 ± 7 AFM indentation Spread morphology on TherFull Paper
2020Chen, Y;NOVEL SIGNALS IN STRESS ERYTHROPOIESIS: COORDINATED CONTROL OF WNT, PGE2 AND PERK SIGNALING PATHWAYS REGULATES EXPANSION AND DIFFERENTIATION OF STRESS ERYTHROID PROGENITORSThesisHuman bone marrow mononuclear cells (MNCs) (ReachBio) were thawed at 37°C water according to the ReachBio instructions. MNCs were enumerated and cell concentrations were adjusted to 5  105 cells/mL. MNCs were cultured in Gibco RPMI-1640 (InvitrogenFull Paper
2020Wu, JL;McIntyre, PW;Hong, JM;Yassen, GH;Bruzzaniti, A;Effects of radiopaque double antibiotic pastes on the proliferation, alkaline phosphatase activity and mineral deposition of dental pulp stem cellsArchives of oral biology32485262Human DPSC were purchased from ALLCells (Alameda, CA, USA), passaged once and aliquots stored in liquid nitrogen. For each experiment, DPSC were thawed and seeded into 10-cm2 dishes and cultured in α-MEM (HyClone Laboratories Inc., South Logan, UT, UFull Paper
2020Madanagopal, TT;Franco-Obregón, A;Rosa, V;Comparative study of xeno-free induction protocols for neural differentiation of human dental pulp stem cells in vitroArchives of oral biology31600663The DPSC were isolated from the third molar from single donor (DPF003, AllCells, USA). Due to ethical requirements, the age, gender and ethnic group of the donor cannot be disclosed SaveFull Paper
2020Pieles, O;Reck, A;Morsczeck, C;High endogenous expression of parathyroid hormone-related protein (PTHrP) supports osteogenic differentiation in human dental follicle cellsHistochemistry and cell biology32710187Human dental follicle cells (DFCs), which were purchased from AllCells, were cultivated in DMEM (Dulbecco's modified Eagle medium) supplemented with 10% fetal bovine serum and SaveFull Paper
2020Fujishiro, A;Iwasa, M;Fujii, S;Maekawa, T;Andoh, A;Tohyama, K;Takaori-Kondo, A;Miura, Y;Menatetrenone facilitates hematopoietic cell generation in a manner that is dependent on human bone marrow mesenchymal stromal/stem cellsInternational journal of hematology32572826Normal human BM samples were purchased from AllCells (Alameda, CA). To confirm consistency among donors, multiple batches of BM-MSCs were isolated as described previously [ SaveFull Paper
2020Yu, CI;Marches, F;Wu, TC;Martinek, J;Palucka, K;Techniques for the generation of humanized mouse models for immuno-oncologyMethods in enzymology32178826Adult CD34 + HPCs from G-CSF mobilized peripheral blood or bone marrow may be obtained from Allcells (Alameda, CA, USA) or Lonza (Basel, Switzerland). Cord blood CD34 + SaveFull Paper
2020Enezei, H;Qabbani, A;Ahmad, A;Khamis, M;Hassani, A;Hamad, H;The Effect of Strontium on Osteoblastogenesis and Osteoclastogenesis in Dental Stem Cells-induced Epidermal Growth Factor at Molecular Level: In Vitro StudyJournal of Hard Tissue BiologyAllCells, LLC Company, Emeryville, CA, USA Dental Stem Cells (DSCs) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Dulbecco’s modified Eagles medium (DMEM) high glucose(4.5g/l) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Fetal bovine serFull Paper
2020Kuang, Z;Li, L;Zhang, P;Chen, B;Wu, M;Ni, H;Yi, S;Zou, J;Liu, J;A novel antibody targeting TIM-3 resulting in receptor internalization for cancer immunotherapyAntibody therapeutics33928230The antibody was purified in-house (Innovent Biologics Co., Ltd., Suzhou, China) from HEK293 cells with either transient or stable expression unless indicated otherwise. Cell culture Human PBMCs (AllCells, Alameda, CA, USA) were cultured in AIM V MFull Paper
2020Moussi, K;Abusamra, DB;Yassine, O;Merzaban, J;Kosel, J;Strain-induced Differentiation of Mesenchymal Stem CellsAnnual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference33018453Human bone marrow derived mesenchymal stem/stromal cells (hMSCs) were purchased from AllCells. All the experiments were performed using MSCs in their 3-5th passage. An SaveFull Paper
2020Yang, Z;Wang, T;Wu, D;Min, Z;Tan, J;Yu, B;RNA N6-methyladenosine reader IGF2BP3 regulates cell cycle and angiogenesis in colon cancerJournal of experimental & clinical cancer research : CR32993738lls were cultured in RPMI 1640 medium supplemented with 10% FBS (Invitrogen, Carlsbad, CA, USA) at 37 °C in a 5% CO2 atmosphere. Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured in EndFull Paper
2020MacGlashan, D;Modulating the Human Basophil Phenotype During Its Development and Maturation: Basophils Derived from In Vitro Cultures of CD34+ Progenitor CellsMethods in molecular biology (Clifton, N.J.)32766967CD34+ progenitor stems (commercially prepared, multiple sources, e.g., AllCells (Quincy, MA, USA), or Fred Hutchinson Cancer Research Center (Seattle, WA, USA)). 12. FBS (fetal bovine serum) (multiple sources, heat-inactivated). 13. Penicillin/streFull Paper
2020Urkasemsin, G;Rungarunlert, S;Ferreira, JN;Bioprinting Strategies for Secretory Epithelial OrganoidsMethods in molecular biology (Clifton, N.J.)32207117hDPSC lines are commercially available through AllCells (Alameda, CA, USA) and should be used at lower passage to guarantee that their proliferation rate and phenotype are stable. 2. The same lot of FBS should be utilized during the all experiments.Full Paper
2020Han, SS;Kang, SW;Metabolic Labeling of Live Stem Cell for In Vitro Imaging and In Vivo TrackingMethods in molecular biology (Clifton, N.J.)30997638Stem cell therapy offers promising solutions to diseases and injuries that traditional medicines and therapies can't effectively cure. To get and explain their full therapeutic potentials, the SaveFull Paper
2020Al-Mohannadi, A;Deola, S;Malki, A;Visualization of Factor Viii with Flow-Cytometry as a tool for Novel Gene Therapy Approach in Hemophilia AUniversity of the Future: Re-Imagining Research and Higher Educationreceived as a kind gift from Antonia Follenzi, Health Sciences Department in Piemonte Orientale University in Novara-Italy. HELA cells (cervical cancer) were purchased from American Type Culture Collection (ATCC™), USA. PBMCs purchased by AllCells (CFull Paper
2020Romito, M;Juillerat, A;Kok, Y;HIldenbeutel, M;Rhiel, M;Andrieux, G;Geiger, J;Rudolph, C;Mussolino, C;Duclert, A;Metzner, K;Duchateau, P;Cathomen, T;Cornu, T;Clinically relevant CCR5 editing in primary CD4+ T cells with TALEN confers resistance to HIV-1 infectionAuthorea, Inc.For oligonucleotide capture assay (OCA), human PBMCs (ALLCELLS, USA) were plated at a density of 1x106 cells/ml in X-vivo-15 media (Lonza, Switzerland) supplemented with 5% SaveFull Paper
2020Turchiano, G;Andrieux, G;Blattner, G;Pennucci, V;Klermund, J;Monaco, G;Poddar, S;Mussolino, C;Cornu, T;Boerries, M;Cathomen, T;Quantitative Evaluation of Chromosomal Rearrangements in Primary Gene-Edited Human Stem Cells by Preclinical CAST-SeqSSRN Electronic JournalCell culture and transfection. Cryopreserved human CD34+ HSPCs derived from cord blood (AllCells, Cat.# CB008F; StemCell-Technologies, Cat.# 70008.3) were thawed and cultivated in a density range of 0.5-1 x 106 /ml at 37°C, 5% CO2 in GMP-grade CellGFull Paper
2020Lu, B;Yang, H;Liu, F;Sun, Y;He, H;Ding, X;Hu, S;xia, l;Escherichia coli Nissle 1917 secreting functional interleukin 2 targets tumours and enhances the immune response to suppress tumoursResearch SquareCT26 colon carcinoma lines were maintained by our laboratory, and peripheral blood mononuclear cells (PBMCs) were purchased from Allcells Biotechnology (Shanghai) Co., Ltd. SaveFull Paper
2020Zhang, Y;Wang, P;wang, t;Fang, Y;Ding, Y;Qian, Q;Chimeric Antigen Receptor T Cells Engineered to Secrete CD40 Agonist Antibodies Enhance Antitumor EfficacyResearch SquareHuman peripheral blood mononuclear cells (PBMC) from healthy donors were purchased from AllCells (Shanghai, China) and cryopreserved in our laboratory. SaveFull Paper
2020Mangiras, D;Mattheakis, P;Ribet, P;Dimitrakopoulos, G;Soft-Clustering Driven Flip-flop Placement Targeting Clock-induced OCVProceedings of the 2020 International Symposium on Physical DesignIn this way, the cluster initialization of clock net n is done on AllCells that includes both the cells connected to n and the pre-defined cluster centers of the hierarchically lower clock sub- SaveFull Paper
2020Li, W;Yang, S;Xu, P;Zhang, D;Tong, Y;Chen, L;Jia, B;Li, A;Ru, D;Zhang, B;Liu, M;Lian, C;Chen, C;Fu, W;Yuan, S;Ren, X;Liang, Y;Yang, Z;Li, W;Wang, S;Zhang, X;Lu, H;Xu, J;Wang, H;Yu, W;Human Identical Sequences of SARS-CoV-2 Promote Clinical Progression of COVID-19 by Upregulating Hyaluronan via NamiRNA-Enhancer NetworkbioRxivHUVEC (human umbilical vein endothelial cell, bought from AllCells) was cultured in the commercial culture medium (AllCells, H-004) according to the manufacturer's guidelines. SaveFull Paper
2020Fiskin, E;Lareau, C;Eraslan, G;Ludwig, L;Regev, A;Single-cell multimodal profiling of proteins and chromatin accessibility using PHAGE-ATACbioRxivFor PBMC and CD8 T cell experiments, cryopreserved PBMCs or CD8 T cells (AllCells) were thawed, washed in PBS and resuspended in cold Flow cytometry buffer (FC buffer; PBS containing 2% FBS). All centrifugation steps were carried out at 350g, 4min, 4Full Paper
2020Mimitou, E;Lareau, C;Chen, K;Zorzetto-Fernandes, A;Takeshima, Y;Luo, W;Huang, T;Yeung, B;Thakore, P;Wing, J;Nazor, K;Sakaguchi, S;Ludwig, L;Sankaran, V;Regev, A;Smibert, P;Scalable, multimodal profiling of chromatin accessibility and protein levels in single cellsbioRxivCryopreserved healthy donor peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BM) were obtained from AllCells (USA) or Cellular Technology Limited (CTL) and processed immediately after thawing. NIH-3T3 and HEK293FT cells were maintainFull Paper
2020Steinbuck, M;Seenappa, L;Jakubowski, A;McNeil, L;Haqq, C;DeMuth, P;A Lymph Node Targeted Amphiphile Vaccine Induces Potent Cellular and Humoral Immunity to SARS-CoV-2bioRxivSARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and ALLCELLS (Alameda, CA), respectively. All samples were received and stored frozen at -80°C until analysisFull Paper
2020Keating, S;Mizrahi, R;Adams, M;Asensio, M;Benzie, E;Carter, K;Chiang, Y;Edgar, R;Gautam, B;Gras, A;Leong, J;Leong, R;Lim, Y;Manickam, V;Medina-Cucurella, A;Niedecken, A;Saini, J;Simons, J;Spindler, M;Stadtmiller, K;Tinsley, B;Wagner, E;Wayham, N;Tracy, L;Lundberg, C;Büscher, D;Terencio, J;Roalfe, L;Pearce, E;Richardson, H;Goldblatt, D;Ramjag, A;Carrington, C;Simmons, G;Muench, M;Chamow, S;Monroe, B;Olson, C;Oguin, T;Lynch, H;Jeanfreau, R;Meyer, E;Adler, A;Johnson, D;Capturing and Recreating Diverse Antibody Repertoires as Multivalent Recombinant Polyclonal Antibody DrugsbioRxivPlasma-derived polyclonal antibodies are polyvalent drugs used for many important clinical indications that require modulation of multiple drug targets simultaneously, including SaveFull Paper
2020Sharma, R;Dever, D;Lee, C;Azizi, A;Pan, Y;Camarena, J;Köhnke, T;Bao, G;Porteus, M;Majeti, R;TRACE-Seq Reveals Clonal Reconstitution Dynamics of Gene Targeted Human Hematopoietic Stem CellsbioRxivFor in vivo studies presented, cord blood-derived CD34 + cells were purchased from AllCells or Stemcell Technologies and were thawed according to the manufacturer's SaveFull Paper
2020Miller, I;Sun, L;Harris, A;Gamboa, L;Zamat, A;Kwong, G;Remote control of CAR T cell therapies by thermal targetingbioRxivPrimary Human CD3+ cells were obtained from an anonymous donor blood after apheresis (AllCells) and were cryopreserved in 90% FBS and 10% DMSO until subsequent use. After SaveFull Paper
2020Mandal, S;Sunagawa, S;Prathipati, P;Belshan, M;Shibata, A;Destache, C;Targeted immuno-antiretroviral HIV therapeutic approach to provide dual protection and boosts cellular immunity: A proof-of-concept studybioRxivHuman immunodeficiency virus (HIV)-infected active and latent CCR5 expressing long-lived T-cells are the primary barrier to HIV/AIDS eradication. Broadly neutralizing antibodies and SaveFull Paper
2020Burley, D;Campagna, C;Harte, A;Kelly, S;Spiegelhoff, M;Effects of Erythrocyte Aggregation on Blood Rheology in Regard to Future Sepsis Diagnosis ApplicationThesisThe preparation of the blood dextran solutions for the micro-ESR, capillary fill, and vibration syllectometry were all done as one big batch. Blood was obtained from AllCells, LLC, a reputable blood and marrow cell supplier from Quincy, MassachusettsFull Paper
2020Fleischer, LC;Engineering Novel Chimeric Antigen Receptors (CARs) for T-cell Malignancies Using Innate Immune CellsThesisMethods and Materials γδ T-cell expansion. gd T cells were expanded from cryopreserved PBMCs purchased from AllCells (Alameda, California) or from fresh PBMCs isolated from 30-50 mL consented, healthy adult blood. PBMCs were isolated from blood usingFull Paper
2020O'Brien, G;Genetic and epigenetic consequences of radiation exposure in human and mouse leukaemogenesisThesisGreece with informed consent from each patient. Bone marrow aspirates were obtained from 5 normal donors (ALLCELLS, Alameda, CA) with informed consent and ethical approval from the Alpha Independent Review Board. Bone marrow aspirates from 7 AML patiFull Paper
2020Kimmey, SC;Mapping Single Cell Protein & Biosynthesis Dynamics Across Human Embryonic & Adult Stem Cell SpecificationThesisFresh bone marrow aspirates from two independent donors were obtained from AllCells (Alameda, CA) on the same day of collection, and immediately after receiving was placed in 37C 5% CO2 incubator for 30 minutes prior to SOM3B labeling. A mixture of aFull Paper
2020Davis-Marcisak, E;Fitzgerald, A;Kessler, M;Danilova, L;Jaffee, E;Zaidi, N;Weiner, L;Fertig, E;Transfer learning between preclinical models and human tumors identifies conserved NK cell activation signature in anti-CTLA-4 responsive tumorsbioRxivFresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selected from donor peripheral blood using CD56 positivity. Donor NK cell purity SaveFull Paper
2020Burgenson, D;Development of a Cell-Free Protein Expression System Derived from Human Blood CellsThesisA comparison between methods used to produce leukapheresis products purchased from Allcells and the New York Blood Center. From the experiments performed, the Allcells SaveFull Paper
2020Yun, ZX;Identification of G9a as a Therapeutically Targetable Driver of Multiple Myeloma TumorigenesisThesisdissociate cells each passage. Cryopreserved Peripheral Blood Mononuclear Cells (PBMC, AllCells) were cultured in RPMI 1640 medium (BioWest, Kansas City, MO) with supplement of 10% FBS (HyClone) and 1% penicillin/streptomycin (Invitrogen) one day befFull Paper
2020Lindgren, A;Mohidin, D;Applying Digital Marketing Methods in the Healthcare Industry: A Case Study at ImmuneedThesisImmuneed Volume Immuneed Traffic Celentyx Volume Celentyx Traffic Reachbio Volume Reachbio Traffic Eurofins Volume Eurofins Traffic Immundnz Volume Immundnz Traffic Nexelis Volume Nexelis Traffic ImmuneCarta Volume ImmuneCarta TrafficFull Paper
2020Su, R;Dong, L;Li, Y;Gao, M;Han, L;Wunderlich, M;Deng, X;Li, H;Huang, Y;Gao, L;Li, C;Zhao, Z;Robinson, S;Tan, B;Qing, Y;Qin, X;Prince, E;Xie, J;Qin, H;Li, W;Shen, C;Sun, J;Kulkarni, P;Weng, H;Huang, H;Chen, Z;Zhang, B;Wu, X;Olsen, MJ;Müschen, M;Marcucci, G;Salgia, R;Li, L;Fathi, AT;Li, Z;Mulloy, JC;Wei, M;Horne, D;Chen, J;Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune EvasionCancer cell32531268After 7 days of selection with 0.5mg/ml G418 Sulfate (10131027, Thermo Fisher Scientific) in ColonyGEL (1201, ReachBio Research Lab), the cells were collected and injected into lethally irradiated (960 rads) 8- to 10-week-old B6.SJL (CD45.1, RRID: IMFull Paper
2020Young, TM;Reyes, C;Pasnikowski, E;Castanaro, C;Wong, C;Decker, CE;Chiu, J;Song, H;Wei, Y;Bai, Y;Zambrowicz, B;Thurston, G;Daly, C;Autophagy protects tumors from T cell-mediated cytotoxicity via inhibition of TNFα-induced apoptosisScience immunology33443027For human tumor cell killing experiments using CD3 bispecific antibody, human T cells were isolated from peripheral blood mononuclear cells (PBMCs) (ReachBio) using Dynabeads Untouched Human T Cells Kit (Thermo Fisher Scientific)Full Paper
2020Shen, C;Sheng, Y;Zhu, AC;Robinson, S;Jiang, X;Dong, L;Chen, H;Su, R;Yin, Z;Li, W;Deng, X;Chen, Y;Hu, YC;Weng, H;Huang, H;Prince, E;Cogle, CR;Sun, M;Zhang, B;Chen, CW;Marcucci, G;He, C;Qian, Z;Chen, J;RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid LeukemiaCell stem cell32402250Thereafter, the transduced cells were then plated into ColonyGEL methylcellulose medium (ReachBio, Seattle, WA) supplied with 10 ng/ml of murine recombinant IL-3, IL-6, GM-CSF and 30 ng/ml of murine recombinant SCF, along with 1.0 mg/ml of G418 (GibcFull Paper
2020Chiu, D;Tavaré, R;Haber, L;Aina, OH;Vazzana, K;Ram, P;Danton, M;Finney, J;Jalal, S;Krueger, P;Giurleo, JT;Ma, D;Smith, E;Thurston, G;Kirshner, JR;Crawford, A;A PSMA-Targeting CD3 Bispecific Antibody Induces Antitumor Responses that Are Enhanced by 4-1BB CostimulationCancer immunology research32184296PBMCs were obtained from ReachBio (WA) for in vivo (ReachBio). C4-2 tumor cells were subcutaneously injected with 5 × 10 6 tumor cells and 1 × 10 6 human PBMCs (ReachBio). SaveFull Paper
2020Patterson, AM;Liu, L;Sampson, CH;Plett, PA;Li, H;Singh, P;Mohammad, KS;Hoggatt, J;Capitano, ML;Orschell, CM;Pelus, LM;A Single Radioprotective Dose of Prostaglandin E2 Blocks Irradiation-Induced Apoptotic Signaling and Early Cycling of Hematopoietic Stem CellsStem cell reports32735825CFCs were quantitated as described by Hoggatt et al. (2013a). TNCs from non-IR mice were plated at 2 × 104 per 35-mm dish in triplicate in ColonyGEL Mouse Complete Medium (Reach Bio, Seattle, WA). Cells from IR mice were plated at 0.5-1 × 106 per disFull Paper
2020Paulson, RF;Hariharan, S;Little, JA;Stress erythropoiesis: definitions and models for its studyExperimental hematology32750404Control PBMCs were purchased from ReachBio. PBMCs obtained were plated at 1 x 10 6 cells/ml stress erythropoiesis differentiation media containing human growth factors. The cells SaveFull Paper
2020Patterson, AM;Plett, PA;Chua, HL;Sampson, CH;Fisher, A;Feng, H;Unthank, JL;Miller, SJ;Katz, BP;MacVittie, TJ;Orschell, CM;Development of a Model of the Acute and Delayed Effects of High Dose Radiation Exposure in Jackson Diversity Outbred Mice; Comparison to Inbred C57BL/6 MiceHealth physics32932286Colony-forming unit cell (CFU-C) analyses Functional BM progenitor number was analyzed utilizing methylcellulose colony assays as per manufacturer instructions (Colony Gel 1202, ReachBio, Seattle, WA)Full Paper
2020Radtke, S;Colonna, L;Perez, AM;Hoffman, M;Kean, LS;Kiem, HP;Isolation of a Highly Purified HSC-enriched CD34+CD90+CD45RA- Cell Subset for Allogeneic Transplantation in the Nonhuman Primate Large-animal ModelTransplantation direct33134503CFC) assays were sorted in purity mode to prevent crosscontamination by different subsets. Colony-forming Cell Assay For CFC assays, 1000 to 1200 sorted cells were seeded into 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA). Hematopoietic colonies werFull Paper
2019Bonner, M;Kanter, J;Macari, E;Lane, R;Lewis, G;Coles, P;Kassenaar, S;Mynampati, S;Schulze, R;Hebert, M;Walters, M;Thompson, A;Asmal, M;Tisdale, J;Pierciey, F;The Relationships between Target Gene Transduction, Engraftment of HSCs and RBC Physiology in Sickle Cell Disease Gene TherapyBloodBackground LentiGlobin for Sickle Cell Disease (SCD) gene therapy contains ex-vivo lentiviral vector (LVV)-mediated addition of a modified β-globin gene (βA-T87Q) into autologous CD34+ hematopoietic stem cells (HSCs). The safety and efFull Paper
2019Kanter, J;Tisdale, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Schmidt, M;Miller, A;Pierciey, F;Huang, W;Ribeil, J;Thompson, A;Walters, M;Resolution of Sickle Cell Disease Manifestations in Patients Treated with Lentiglobin Gene Therapy: Updated Results from the Phase 1/2 Hgb-206 Group C StudyBloodBackground β-globin gene transfer into hematopoietic stem cells (HSCs) could reduce or eliminate sickle cell disease (SCD)-related manifestations. LentiGlobin for SCD gene therapy contains autologous CD34+ cells transduced with the BB3Full Paper
2019Walters, M;Tisdale, J;Kwiatkowski, J;Krishnamurti, L;Mapara, M;Schmidt, M;Miller, A;Pierciey, F;Huang, W;Ribeil, J;Kanter, J;Thompson, A;Exploring the Drivers of Potential Clinical Benefit in Initial Patients Treated in the Hgb-206 Study of Lentiglobin for Sickle Cell Disease (SCD) Gene TherapyBloodBackground LentiGlobin for SCD gene therapy contains autologous CD34+ hematopoietic stem cells (HSCs) transduced with the BB305 lentiviral vector (LVV) encoding β-globin with an anti-sickling substitution (T87Q). Its safety and efficacFull Paper
2019Eapen, M;Neuberg, D;Mendizabal, A;Stevenson, K;Antin, J;DiFronzo, N;El Rassi, F;Lulla, P;Waller, E;Garrison, J;Smith, S;Sullivan, K;Walters, M;Krishnamurti, L;A Phase II Trial to Compare Allogeneic Transplant Vs. Standard of Care for Severe Sickle Cell Disease: Blood and Marrow Transplant Clinical Trials Network (BMT CTN) Protocol 1503BloodBackground: Allogeneic hematopoietic cell transplantation (HCT) is a curative treatment for sickle cell disease (SCD). A pilot trial confirmed the suitability of a myeloablative conditioning regimen (busulfan/fludarabine/r-ATG) for HLA-matched siblinFull Paper
2019Loi, S;Giobbie-Hurder, A;Gombos, A;Bachelot, T;Hui, R;Curigliano, G;Campone, M;Biganzoli, L;Bonnefoi, H;Jerusalem, G;Bartsch, R;Rabaglio-Poretti, M;Kammler, R;Maibach, R;Smyth, M;Di Leo, A;Colleoni, M;Viale, G;Regan, M;André, F;Fumagalli, D;Gelber, R;Goulioti, T;Hiltbrunner, A;Hui, R;Roschitzki, H;Ruepp, B;Boyle, F;Stahel, R;Aebi, S;Coates, A;Goldhirsch, A;Karlsson, P;Kössler, I;Fournarakou, S;Gasca, A;Pfister, R;Ribeli-Hofmann, S;Weber, M;Celotto, D;Comune, C;Frapolli, M;Sánchez-Hohl, M;Huang, H;Mahoney, C;Price, K;Scott, K;Shaw, H;Fischer, S;Greco, M;King, C;Andrighetto, S;Piccart-Gebhart, M;Findlay, H;Jenkins, M;Karantza, V;Mejia, J;Schneier, P;Pembrolizumab plus trastuzumab in trastuzumab-resistant, advanced, HER2-positive breast cancer (PANACEA): a single-arm, multicentre, phase 1b–2 trialThe Lancet OncologyPD-L1 status for the first 79 patients was assessed with the QualTek immunohistochemistry assay (QualtTek Molecular Laboratories, Santa Barbara, CA, USA) in which PD-L1 staining in at least 1% of tumour cells or any staining in stroma were defined asFull Paper
2019Kassim, A;Walters, M;Eapen, M;Allison, B;Mendizabal, A;Brodsky, R;DeBaun, M;Reduced Intensity Conditioning for Haploidentical Bone Marrow Transplantation in Patients with Symptomatic Sickle Cell Disease: BMT CTN Protocol 1507BloodBackground: Hematopoietic cell transplantation (HCT) has curative potential in sickle cell disease (SCD). A reduced intensity haploidentical bone marrow transplant (haplo-BMT) platform piloted by investigators at Johns Hopkins, addressed key barriersFull Paper
2019Nghiem, P;Bhatia, S;Lipson, EJ;Sharfman, WH;Kudchadkar, RR;Brohl, AS;Friedlander, PA;Daud, A;Kluger, HM;Reddy, SA;Boulmay, BC;Riker, AI;Burgess, MA;Hanks, BA;Olencki, T;Margolin, K;Lundgren, LM;Soni, A;Ramchurren, N;Church, C;Park, SY;Shinohara, MM;Salim, B;Taube, JM;Bird, SR;Ibrahim, N;Fling, SP;Homet Moreno, B;Sharon, E;Cheever, MA;Topalian, SL;Durable Tumor Regression and Overall Survival in Patients With Advanced Merkel Cell Carcinoma Receiving Pembrolizumab as First-Line TherapyJ. Clin. Oncol.3072617521,22. PD-L1 IHC. PD-L1 staining (anti-PD-L1 clone 22C3; Merck Research Laboratories, Kenilworth, NJ) was performed at QualTek Molecular Laboratories (Newtown, PA) on formalin-fixed paraffin-embedded sections from pretreatment MCC biopsies, as previoFull Paper
2019Fedoriw, A;Rajapurkar, SR;O'Brien, S;Gerhart, SV;Mitchell, LH;Adams, ND;Rioux, N;Lingaraj, T;Ribich, SA;Pappalardi, MB;Shah, N;Laraio, J;Liu, Y;Butticello, M;Carpenter, CL;Creasy, C;Korenchuk, S;McCabe, MT;McHugh, CF;Nagarajan, R;Wagner, C;Zappacosta, F;Annan, R;Concha, NO;Thomas, RA;Hart, TK;Smith, JJ;Copeland, RA;Moyer, MP;Campbell, J;Stickland, K;Mills, J;Jacques-O'Hagan, S;Allain, C;Johnston, D;Raimondi, A;Porter Scott, M;Waters, N;Swinger, K;Boriack-Sjodin, A;Riera, T;Shapiro, G;Chesworth, R;Prinjha, RK;Kruger, RG;Barbash, O;Mohammad, HP;Anti-tumor Activity of the Type I PRMT Inhibitor, GSK3368715, Synergizes with PRMT5 Inhibition through MTAP LossCancer Cell31257072GSK3368715 was evaluated at 20, 5, 1.25, 0.3125 and 0.078 mM in a total of 10 patient samples. DLBCL patient cells from 10 unique donors were received as frozen samples from Conversant Bio. The human biological samples were sourced ethically and theiFull Paper
2019Leighl, NB;Hellmann, MD;Hui, R;Carcereny, E;Felip, E;Ahn, MJ;Eder, JP;Balmanoukian, AS;Aggarwal, C;Horn, L;Patnaik, A;Gubens, M;Ramalingam, SS;Lubiniecki, GM;Zhang, J;Piperdi, B;Garon, EB;Pembrolizumab in patients with advanced non-small-cell lung cancer (KEYNOTE-001): 3-year results from an open-label, phase 1 studyLancet Respir Med30876831NR=not reached. PD-L1=programmed death ligand 1. TPS=tumour proportion score. *PD-L1 TPS 1% on the basis of the QualTek assay (Molecular Laboratories, GoletaFull Paper
2019Melamed, Z;López-Erauskin, J;Baughn, MW;Zhang, O;Drenner, K;Sun, Y;Freyermuth, F;McMahon, MA;Beccari, MS;Artates, JW;Ohkubo, T;Rodriguez, M;Lin, N;Wu, D;Bennett, CF;Rigo, F;Da Cruz, S;Ravits, J;Lagier-Tourenne, C;Cleveland, DW;Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegenerationNat. Neurosci.30643298... titute for the kind contribution of the SIN18 vector. We thank B. Ren for providing the Illumina sequencing platform. We thank A. Goginashvili for valued input on the manuscript. We thank J. Nuovo at Phylogeny Inc. (Columbus, OH) for performing tFull Paper
2019Thompson, A;Walters, M;Kwiatkowski, J;Hongeng, S;Porter, J;Sauer, M;Thrasher, A;Thuret, I;Elliot, H;Tao, G;Colvin, R;Locatelli, F;Northstar-2: Updated Safety and Efficacy Analysis of Lentiglobin Gene Therapy in Patients with Transfusion-Dependent β-Thalassemia and Non-β0/β0 GenotypesBloodBackground Transfusion-dependent β-thalassemia (TDT) is treated with regular, lifelong red blood cell (RBC) transfusions and despite iron-chelating therapy, carries a risk of serious organ damage from iron overload and other complicatiFull Paper
2019Petermann, F;Pękowska, A;Johnson, CA;Jankovic, D;Shih, HY;Jiang, K;Hudson, WH;Brooks, SR;Sun, HW;Villarino, AV;Yao, C;Singleton, K;Akondy, RS;Kanno, Y;Sher, A;Casellas, R;Ahmed, R;O'Shea, JJ;The Magnitude of IFN-γ Responses Is Fine-Tuned by DNA Architecture and the Non-coding Transcript of Ifng-as1Mol. CellFor LCMV experiments RNA was isolated from sorted cells of LCMV-infected mice with the QIAGEN AllPrep Micro Kit. Libraries were prepared and sequenced by the HudsonAlpha Genomic Services Lab (paired-end, 150 bp reads). For all other experiments totalFull Paper
2019Kwiatkowski, J;Thompson, A;Rasko, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;Schmidt, M;Kletzel, M;Vichinsky, E;Deary, B;Chen, Y;Petrusich, A;Walters, M;Long-Term Clinical Outcomes of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia in the Northstar (HGB-204) StudyBloodBackground Patients with transfusion-dependent β-thalassemia (TDT) may experience transfusional iron overload and end-organ damage. While potentially curative, allogeneic hematopoietic stem cell (HSC) transplantation is limited by tranFull Paper
2019Furie, R;Werth, VP;Merola, JF;Stevenson, L;Reynolds, TL;Naik, H;Wang, W;Christmann, R;Gardet, A;Pellerin, A;Hamann, S;Auluck, P;Barbey, C;Gulati, P;Rabah, D;Franchimont, N;Monoclonal antibody targeting BDCA2 ameliorates skin lesions in systemic lupus erythematosusJ. Clin. Invest.30645203... un-exposed skin of HVs matched approximately by age and ethnicity to the BIIB059-treated cohort (mean age = 34; range, 28–37; White, 6/8; Asian, 1/8; Black or African American, 1/8; female, 8/8) by Folio Biosciences, then processed into paraffinFull Paper
2019Hudson, WH;Prokhnevska, N;Gensheimer, J;Akondy, R;McGuire, DJ;Ahmed, R;Kissick, HT;Expression of novel long noncoding RNAs defines virus-specific effector and memory CD8+ T cellsNat Commun30643116... of Emory University. RNA Isolation and sequencing RNA was isolated from sorted cells of LCMV-infected mice with the Qiagen AllPrep Micro Kit. Library preparation and sequencing were performed by the HudsonAlpha Genomic Services Laboratory via thFull Paper
2019Ma, X;Shao, Y;Tian, L;Flasch, DA;Mulder, HL;Edmonson, MN;Liu, Y;Chen, X;Newman, S;Nakitandwe, J;Li, Y;Li, B;Shen, S;Wang, Z;Shurtleff, S;Robison, LL;Levy, S;Easton, J;Zhang, J;Analysis of error profiles in deep next-generation sequencing dataGenome Biol.30867008Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA. Xiaotu.Ma@stjude.org.++Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.++Department of ComputatioFull Paper
2019Zeng, Q;Liao, C;Terhune, J;Wang, L;Impacts of florfenicol on the microbiota landscape and resistome as revealed by metagenomic analysisMicrobiome31818316Carlsbad, CA) according to the manufacturer's instructions. The library construction and sequencing were conducted at the HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). Genomic libraries were prepared with theFull Paper
2019Lee, CR;Yonk, AJ;Wiskerke, J;Paradiso, KG;Tepper, JM;Margolis, DJ;Opposing Influence of Sensory and Motor Cortical Input on Striatal Circuitry and Choice BehaviorCurr. Biol.30982651Light was delivered to the cortical afferents and PV interneurons in the striatum through an optical fiber patchcord (Thorlabs; length 1 m; 0.50 nA; 200 mm diameter) connected to an optical cannula (described above) via a mating sleeve (Thorlabs). AFull Paper
2019Ramachandran, I;Lowther, DE;Dryer-Minnerly, R;Wang, R;Fayngerts, S;Nunez, D;Betts, G;Bath, N;Tipping, AJ;Melchiori, L;Navenot, JM;Glod, J;Mackall, CL;D'Angelo, SP;Araujo, DM;Chow, WA;Demetri, GD;Druta, M;Van Tine, BA;Grupp, SA;Abdul Razak, AR;Wilky, B;Iyengar, M;Trivedi, T;Winkle, EV;Chagin, K;Amado, R;Binder, GK;Basu, S;Systemic and local immunity following adoptive transfer of NY-ESO-1 SPEAR T cells in synovial sarcomaJ Immunother Cancer31651363d Cross (Philadelphia, PA). NY-ESO-1 testing was performed via IHC at a Clinical Laboratory Improvement Amendments certified pathology laboratory at the National Cancer Institute (Bethesda, MD) or at QualTek Labs (Goleta, CA). Disease response was clFull Paper
2019Spino, M;Kurz, SC;Chiriboga, L;Serrano, J;Zeck, B;Sen, N;Patel, S;Shen, G;Vasudevaraja, V;Tsirigos, A;Suryadevara, CM;Frenster, JD;Tateishi, K;Wakimoto, H;Jain, R;Riina, HA;Nicolaides, TP;Sulman, EP;Cahill, DP;Golfinos, JG;Isse, K;Saunders, LR;Zagzag, D;Placantonakis, DG;Snuderl, M;Chi, AS;Cell Surface Notch Ligand DLL3 is a Therapeutic Target in Isocitrate Dehydrogenase-mutant GliomaClin. Cancer Res.30397180For the discovery set, 20 nontumor brain tissue samples and 63 glioma tumor samples were obtained from Cooperative Human Tumor Network (CHTN), Conversant Bio, and RS Diagnostics. This set of tumors included the following diagnoses: glioblastoma, WHOFull Paper
2019Habra, MA;Stephen, B;Campbell, M;Hess, K;Tapia, C;Xu, M;Rodon Ahnert, J;Jimenez, C;Lee, JE;Perrier, ND;Boraddus, RR;Pant, S;Subbiah, V;Hong, DS;Zarifa, A;Fu, S;Karp, DD;Meric-Bernstam, F;Naing, A;Phase II clinical trial of pembrolizumab efficacy and safety in advanced adrenocortical carcinomaJ Immunother Cancer31533818PD-L1 staining was performed by Qualtek using Merck 22C3 antibody for PD-L1 and scored by a board-certified pathologist. Based on the percentage and intensity of membrane staining, H-score, ranging from 0 to 300, was assigned to tumor samples ;iFull Paper
2019Lv, JW;Li, JY;Luo, LN;Wang, ZX;Chen, YP;Comparative safety and efficacy of anti-PD-1 monotherapy, chemotherapy alone, and their combination therapy in advanced nasopharyngeal carcinoma: findings from recent advances in landmark trialsJ Immunother Cancer31238988D-L1 expression was assessed baseline on an archived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy sample using a laboratory-developed prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA). PD-L1Full Paper
2019Clouthier, DL;Lien, SC;Yang, SYC;Nguyen, LT;Manem, VSK;Gray, D;Ryczko, M;Razak, ARA;Lewin, J;Lheureux, S;Colombo, I;Bedard, PL;Cescon, D;Spreafico, A;Butler, MO;Hansen, AR;Jang, RW;Ghai, S;Weinreb, I;Sotov, V;Gadalla, R;Noamani, B;Guo, M;Elston, S;Giesler, A;Hakgor, S;Jiang, H;McGaha, T;Brooks, DG;Haibe-Kains, B;Pugh, TJ;Ohashi, PS;Siu, LL;An interim report on the investigator-initiated phase 2 study of pembrolizumab immunological response evaluation (INSPIRE)J Immunother Cancer30867072IHC. For screening biopsies only, the FFPE blocks were used for PD-L1 IHC (clone 22C3) on 4-5 μm sections mounted on positively charged ProbeOn slides (QualTek, Goleta, CA). QualTek provided a modified proportion score ;from the same site as theFull Paper
2019Rowley, MJ;Lyu, X;Rana, V;Ando-Kuri, M;Karns, R;Bosco, G;Corces, VG;Condensin II Counteracts Cohesin and RNA Polymerase II in the Establishment of 3D Chromatin OrganizationCell Rep30865881We would like to thank the HudsonAlpha Institute for Biotechnology Genomic Services Lab for their help with Illumina sequencing. This work was supported by NIH Pathway to Independence Award K99/R00 GM127671 (M.J.R.) and U.S. Public Health Service AwaFull Paper
2019Wu, T;Dejanovic, B;Gandham, VD;Gogineni, A;Edmonds, R;Schauer, S;Srinivasan, K;Huntley, MA;Wang, Y;Wang, TM;Hedehus, M;Barck, KH;Stark, M;Ngu, H;Foreman, O;Meilandt, WJ;Elstrott, J;Chang, MC;Hansen, DV;Carano, RAD;Sheng, M;Hanson, JE;Complement C3 Is Activated in Human AD Brain and Is Required for Neurodegeneration in Mouse Models of Amyloidosis and TauopathyCell Rep31433986Human CSF cohort 1 Folio Biosciences n/a Human CSF cohort 2 PrecisionMed n/a Frozen human brain tissue Folio Biosciences n/Full Paper
2019Angelova, E;Audette, C;Kovtun, Y;Daver, N;Wang, SA;Pierce, S;Konoplev, SN;Khogeer, H;Jorgensen, JL;Konopleva, M;Zweidler-McKay, PA;Medeiros, LJ;Kantarjian, HM;Jabbour, EJ;Khoury, JD;CD123 expression patterns and selective targeting with a CD123-targeted antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemiaHaematologica30361418... In vitro evaluation of primary B-cell acute lymphoblastic leukemia samples Bone marrow mononuclear cells from 11 newly diagnosed and 10 relapsed/refractory B-ALL patients were obtained from MDACC or ConversantBio. The number of CD123 antibody-biFull Paper
2019Bao, L;Tian, C;Liu, S;Zhang, Y;Elaswad, A;Yuan, Z;Khalil, K;Sun, F;Yang, Y;Zhou, T;Li, N;Tan, S;Zeng, Q;Liu, Y;Li, Y;Li, Y;Gao, D;Dunham, R;Davis, K;Waldbieser, G;Liu, Z;The Y chromosome sequence of the channel catfish suggests novel sex determination mechanisms in teleost fishBMC Biol.30683095;... Bioanalyzer. Equal amounts of RNA from each 5-day period were pooled from 10 to 14 dpf and 15 to 19 dpf for male and female, respectively. These four pooled samples were outsourced for RNA-Seq at the HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2019Rieker, C;Migliavacca, E;Vaucher, A;Mayer, FC;Baud, G;Marquis, J;Charpagne, A;Hegde, N;Guignard, L;McLachlan, M;Pooler, AM;Apolipoprotein E4 Expression Causes Gain of Toxic Function in Isogenic Human Induced Pluripotent Stem Cell-Derived Endothelial CellsArterioscler. Thromb. Vasc. Biol.31315437Immunohistochemistry Analysis of Postmortem Human Brain Tissue Human hippocampal brain tissue from AD (Braak stage V-VI; both male and female) individuals was purchased from Folio Biosciences, genotyped for ApoE, and processed for cryo- sectioningFull Paper
2019Smith, A;Schiller, G;Vercellotti, G;Kwiatkowski, J;Krishnamurti, L;Esrick, E;Williams, D;Miller, W;Woolfson, A;Walters, M;Preliminary Results of a Phase 1/2 Clinical Study of Zinc Finger Nuclease-Mediated Editing of BCL11A in Autologous Hematopoietic Stem Cells for Transfusion-Dependent Beta ThalassemiaBloodIntroduction: Persistently high fetal hemoglobin (HbF) expression can ameliorate severe transfusion-dependent beta thalassemia (TDT). BCL11A, a master regulator of the fetal-to-adult hemoglobin switch, is a rational gene-editing target in beta globinFull Paper
2019Kang, J;Yeom, G;Jang, H;Oh, J;Park, CJ;Kim, MG;Development of Replication Protein A-Conjugated Gold Nanoparticles for Highly Sensitive Detection of Disease BiomarkersAnal. Chem.31269392Patient samples for NP and cTnI were obtained from Discovery Life Sciences, Inc. The study protocol was thoroughly explained to the patients, and signed written informed consent forms were obtained in accordance with the approved guidelines and relevFull Paper
2019Planes-Laine, G;Rochigneux, P;Bertucci, F;Chrétien, AS;Viens, P;Sabatier, R;Gonçalves, A;PD-1/PD-L1 Targeting in Breast Cancer: The First Clinical Evidences Are Emerging. A Literature ReviewCancers (Basel)31336685ts included locally advanced or metastatic disease, after a failure of or inability to receive standard therapy, ECOG PS 0 or 1, ≥ 1 measurable lesion and PD-L1 positivity, using a prototype assay (QualTek Molecular Laboratories, Goleta, CA, USA) andFull Paper
2019Bernard-Gauthier, V;Mossine, AV;Knight, A;Patnaik, D;Zhao, WN;Cheng, C;Krishnan, HS;Xuan, LL;Chindavong, PS;Reis, SA;Chen, JM;Shao, X;Stauff, J;Arteaga, J;Sherman, P;Salem, N;Bonsall, D;Amaral, B;Varlow, C;Wells, L;Martarello, L;Patel, S;Liang, SH;Kurumbail, RG;Haggarty, SJ;Scott, PJH;Vasdev, N;Structural Basis for Achieving GSK-3β Inhibition with High Potency, Selectivity, and Brain Exposure for Positron Emission Tomography Imaging and Drug DiscoveryJ. Med. Chem.31535859For autoradiography experiments, fresh-frozen postmortem human brain tissue blocks were acquired from Folio-Conversant and the University of California at San Francisco NDBB. In vitro autoradiography studies were performed as described in the SupportFull Paper
2019McCoy, CR;Glover, ME;Flynn, LT;Simmons, RK;Cohen, JL;Ptacek, T;Lefkowitz, EJ;Jackson, NL;Akil, H;Wu, X;Clinton, SM;Altered DNA Methylation in the Developing Brains of Rats Genetically Prone to High versus Low AnxietyJ. Neurosci.30683683Starting material for the enrichment protocol was 1g of sonicated DNA, and captured fragments were eluted using 2500 mM NaCl per the manufacturer’s instructions and as described previously (Brinkman et al., 2010). Captured material was purified usingFull Paper
2019Chiu, H;Trisal, P;Bjorklund, C;Carrancio, S;Toraño, EG;Guarinos, C;Papazoglou, D;Hagner, PR;Beldi-Ferchiou, A;Tarte, K;Delfau-Larue, MH;Morschhauser, F;Ramsay, AG;Gandhi, AK;Combination lenalidomide-rituximab immunotherapy activates anti-tumour immunity and induces tumour cell death by complementary mechanisms of action in follicular lymphomaBr. J. Haematol.30767211Viably‐frozen PBMC from naive and relapsed/refractory FL and MZL patients were purchased from ConversantBio (Huntsville, AL, USA), ProteoGenex, (Inglewood, CA, USA) and BioreclammationIVT (Hicksville, NY, USA). Cell lines and cell cultureFull Paper
2019Liang, X;Zhuang, J;Löffler, FE;Zhang, Y;DeBruyn, JM;Wilhelm, SW;Schaeffer, SM;Radosevich, M;Viral and bacterial community responses to stimulated Fe(III)-bioreduction during simulated subsurface bioremediationEnviron. Microbiol.30773777Whole soil DNA was obtained by extracting both soil aggregates and associated pore water (Cápiro et al., 2014) using PowerLyser PowerSoil DNA isolation kit (MoBio Laboratories Inv. Carlsbad, CA) following the manufacturer’s instructions. The extracteFull Paper
2019Nowinski, B;Motard-Côté, J;Landa, M;Preston, CM;Scholin, CA;Birch, JM;Kiene, RP;Moran, MA;Microdiversity and temporal dynamics of marine bacterial dimethylsulfoniopropionate genesEnviron. Microbiol.30761723This article is protected by copyright. All rights reserved. Page 23. HiSeq Rapid Run at HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA). Metagenomic data are deposited in the NCBI SRA under project number PRJNA505827. Single AmplifiedFull Paper
2019Xu, H;Muise, ES;Javaid, S;Chen, L;Cristescu, R;Mansueto, MS;Follmer, N;Cho, J;Kerr, K;Altura, R;Machacek, M;Nicholson, B;Addona, G;Kariv, I;Chen, H;Identification of predictive genetic signatures of Cytarabine responsiveness using a 3D acute myeloid leukaemia modelJ. Cell. Mol. Med.314493472 METHODS. 2.1 Cells and reagents. Normal and AML patient BMMCs were purchased from Folio Conversant and ProteoGenex. Recombinant human fibronectin was purchased from Millipore. Rat tail collagen type 1 and matrigel were purchased from Corning IncFull Paper
2019Kim, YJ;Keam, B;Ock, CY;Song, S;Kim, M;Kim, SH;Kim, KH;Kim, JS;Kim, TM;Kim, DW;Lee, JS;Heo, DS;A phase II study of pembrolizumab and paclitaxel in patients with relapsed or refractory small-cell lung cancerLung Cancer31494530Informed consent for specimens be obtained during screening for protocol enrollment from all subjects or legally acceptable representative, at a trial visit by the investigator or designate. Pretreatment blood sampling and tumor biopsies will be doneFull Paper
2019Yang, X;Saito, Y;Rao, A;Kim, HJ;Singh, P;Scott, E;Larson, M;Pan, W;Desai, M;Hubbell, E;Alignment-free filtering for cfNA fusion fragmentsBioinformatics31510681We have obtained 40 ml of whole blood collected in Streck tubes, separated into plasma and extracted cfRNA samples from two Stage IV Prostate cancer patients and three healthy controls from Conversant Bio and generated cfRNA sequencing data with UMIsFull Paper
2019Qi, Z;Wang, L;Desai, K;Cogswell, J;Stern, M;Lawson, B;Kerkar, SP;Vitazka, P;Reliable Gene Expression Profiling from Small and Hematoxylin and Eosin-Stained Clinical Formalin-Fixed, Paraffin-Embedded Specimens Using the HTG EdgeSeq PlatformJ Mol Diagn31255795Rockville, MD). Small-cell lung cancer (SCLC) samples (n = 4) were purchased from Conversant Biologics, Inc. (Huntsville, AL) or Proteogenex (Inglewood, CA). FFPE muscle tissue was obtained from Proteogenex. RNA samplesFull Paper
2019Dietz, L;Dömel, JS;Leese, F;Mahon, AR;Mayer, C;Phylogenomics of the longitarsal Colossendeidae: The evolutionary history of an Antarctic sea spider radiationMol. Phylogenet. Evol.31002869Sea spiders (Pycnogonida) constitute a group of marine benthic arthropods that has a particularly high species diversity in the Southern Ocean. The "longitarsal" group of the sea spider family Colossendeidae is especially abundant in this region. HowFull Paper
2019Varga, A;Piha-Paul, S;Ott, PA;Mehnert, JM;Berton-Rigaud, D;Morosky, A;Yang, P;Ruman, J;Matei, D;Pembrolizumab in patients with programmed death ligand 1-positive advanced ovarian cancer: Analysis of KEYNOTE-028Gynecol. Oncol.30522700disease based on Response Evaluation Criteria in Solid Tumors version 1.1 (RECIST v1.1), PD-L1 positivity, determined immunohistochemically and defined as membranous staining on ≥1% modified proportion score or interface pattern (QualTek Molecular LaFull Paper
2019Lee, MJ;Miller, Z;Park, JE;Bhattarai, D;Lee, W;Kim, KB;H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growthSci Rep30858500... on-linear regression based on n = 3 replicates per compound per concentration. (d) Heat map showing proteasome catalytic subunit activity profiles of 6 PI-naïve patient MM samples purchased from Conversant Biologics and AllCells (left). The numbeFull Paper
2019Liang, X;Radosevich, M;Löffler, F;Schaeffer, SM;Zhuang, J;Impact of microbial iron oxide reduction on the transport of diffusible tracers and non-diffusible nanoparticles in soilsChemosphere30597359Inc. Carlsbad, California, USA). The DNA samples were quantified using PicoGreen Assay Kit (Carlsbad, California, USA) and sent out for sequencing at HudsonAlpha Genomic Services Lab (Huntsville, Alabama, USA). TheFull Paper
2019Nicolini, F;Bocchini, M;Bronte, G;Delmonte, A;Guidoboni, M;Crinò, L;Mazza, M;Malignant Pleural Mesothelioma: State-of-the-Art on Current Therapies and Promises for the FutureFront Oncol32039010With a centrally-assessed 22C3 antibody-based immunohistochemistry assay (Qualtek laboratory), PDL1 expression was available in 62 patients: 45% were found negative (Full Paper
2019Gani, AW;Wei, W;Shi, RZ;Ng, E;Nguyen, M;Chua, MS;So, S;Wang, SX;An Automated, Quantitative, and Multiplexed Assay Suitable for Point-of-Care Hepatitis B Virus DiagnosticsSci Rep31666635HBV DNA levels). As controls, we recruited 5 healthy volunteers who had no prior history of HBV and who were vaccinated against HBV. Serum samples from healthy Asian donors were also purchased from Conversant Biologics (Huntsville, AL). SupplementaFull Paper
2019Li, Y;Kocot, KM;Tassia, MG;Cannon, JT;Bernt, M;Halanych, KM;Mitogenomics Reveals a Novel Genetic Code in HemichordataGenome Biol Evol30476024Variant codes in mitochondrial genomes within deuterostomes and photos from representatives of hemichordates. (A) Phylogenetic relationships according to the current understanding of deuterostome phylogeny based on multiple studies, red bars show codFull Paper
2019Juttukonda, LJ;Green, ER;Lonergan, ZR;Heffern, MC;Chang, CJ;Skaar, EP;Acinetobacter baumannii OxyR Regulates the Transcriptional Response to Hydrogen PeroxideInfect. Immun.30297527RNA-seq library construction and sequencing were performed by HudsonAlpha (Huntsville, AL). The concentration and integrity of the total RNA were estimated with a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA) and an Agilent 2100 bioanalyzer (AppliFull Paper
2019Patel, S;Knight, A;Krause, S;Teceno, T;Tresse, C;Li, S;Cai, Z;Gouasmat, A;Carroll, VM;Barret, O;Gottmukkala, V;Zhang, W;Xiang, X;Morley, T;Huang, Y;Passchier, J;Preclinical In Vitro and In Vivo Characterization of Synaptic Vesicle 2A-Targeting Compounds Amenable to F-18 Labeling as Potential PET Radioligands for Imaging of Synapse IntegrityMol Imaging Biol31728839The same procedure was used for human post-mortem tissue (Folio Biosciences, Miami Brain Bank, and UCSF Memory and Aging Center, USA). In Vitro Homogenate Binding. All homogenate studies were carried out in 96-well format as previously described [14]Full Paper
2019Gao, L;Yuan, Z;Zhou, T;Yang, Y;Gao, D;Dunham, R;Liu, Z;FOXO genes in channel catfish and their response after bacterial infectionDev. Comp. Immunol.30905685; Plus Kit (Qiagen). RNA-seq library was prepared and sequenced by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) using an Illumina HiSeq 2000 instrument with 100 bp paired end (PE) reads. The trimmed highFull Paper
2019Havird, JC;Meyer, E;Fujita, Y;Vaught, RC;Henry, RP;Santos, SR;Disparate responses to salinity across species and organizational levels in anchialine shrimpsJ. Exp. Biol.31727759Assembly of reference transcriptomes was previously described for H. rubra (Havird and Santos, 2016a) and the four species from Japan (Havird et al., 2014b). Briefly, total RNA was extracted from whole animals 1 month after preservation in RNAlater vFull Paper
2019Rey, EG;Finkelstein, JL;Erickson, D;Fluorescence lateral flow competitive protein binding assay for the assessment of serum folate concentrationsPLoS ONE31166978Serum sample preparation. Calibration curves made with human serum spiked with FA and 5-MTHF were performed as part of a previous work[26]. Human serum samples of varying folate concentration were purchased from Discovery Life Sciences Inc. (Los OsosFull Paper
2019Mehnert, JM;Varga, A;Brose, MS;Aggarwal, RR;Lin, CC;Prawira, A;de Braud, F;Tamura, K;Doi, T;Piha-Paul, SA;Gilbert, J;Saraf, S;Thanigaimani, P;Cheng, JD;Keam, B;Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with advanced, PD-L1-positive papillary or follicular thyroid cancerBMC Cancer30832606biopsy sample. PD-L1 expression was assessed using a prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, California) [22] and the 22C3 antibody (Merck & Co., Inc., Kenilworth, New Jersey). PD ;screening period using eiFull Paper
2019Huang, RSP;Holmes, BF;Powell, C;Marati, RV;Tyree, D;Admire, B;Streator, A;Newell, AEH;Perez, J;Dalvi, D;ElGabry, EA;Delta-like Protein 3 Prevalence in Small Cell Lung Cancer and DLL3 (SP347) Assay CharacteristicsArch. Pathol. Lab. Med.30958693formalin), paraffin-embedded SCLC whole-section samples was procured from an internal tissue bank at Ventana Medical Systems, Inc, and the following commercial tissue banks: Asterand Bioscience; Avaden BioSciences; Boca Biolistics; Conversant Bio; GLFull Paper
2019Tsypin, M;Asmellash, S;Meyer, K;Touchet, B;Roder, H;Extending the information content of the MALDI analysis of biological fluids via multi-million shot analysisPLoS ONE31815946100 serum samples were purchased from the commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrics (Fort Worth, TX). Samples were collected under ethics-approved protocols according to the requirements of Conversant Bio and Oncology MFull Paper
2019Li, S;Song, C;Yang, S;Yu, W;Zhang, W;Zhang, G;Xi, Z;Lu, E;Supercritical CO2 foamed composite scaffolds incorporating bioactive lipids promote vascularized bone regeneration via Hif-1α upregulation and enhanced type H vessel formationActa biomaterialia31154054Upon reaching 80-90% confluency, the rBMSCs were passaged with passages 2-4 being used for further experiments. HUVECs (AllCells, Shanghai, China) were cultured in endothelial cell medium (ECM, ScienCell, USA) under the same conditions and used for iFull Paper
2019Desai, SP;Coston, A;Berlin, A;Micro-Electrical Impedance Spectroscopy and Identification of Patient-Derived, Dissociated Tumor CellsIEEE Trans Nanobioscience31180894Stored waveforms are further analyzed using statistical data analysis techniques. C. Dissociated Tumor Cells and Cell Culture Dissociated tumor cells (DTCs) were acquired directly from Conversant Bio (Huntsville, AL) for a range of indications and caFull Paper
2019Lee, JS;Rosoff, D;Luo, A;Longley, M;Phillips, M;Charlet, K;Muench, C;Jung, J;Lohoff, FW;PCSK9 is Increased in Cerebrospinal Fluid of Individuals With Alcohol Use DisorderAlcohol. Clin. Exp. Res.30933362the NIH Institutional Review Board (IRB). All participants provided written informed consent before participation. CSF of deidentified controls was purchased from Discovery Life Sciences, Inc. (Research Biospecimen Sample Bank, Los Osos, CA, USA)Full Paper
2019Burbelo, PD;Chaturvedi, A;Notkins, AL;Gunti, S;Luciferase-Based Detection of Antibodies for the Diagnosis of HPV-Associated Head and Neck Squamous Cell CarcinomaDiagnostics (Basel)31390810The serum samples from colon cancer (CC), systemic lupus erythematosus (SLE), and HNSCC patients used in this report were obtained from Conversant Bio (601 Genome Way Suite 1200, Huntsville, Alabama 35806). SubjectsFull Paper
2019Kao, JH;Lin, CY;Chuang, WL;Cheng, YY;Hu, JY;Liang, WK;Friebe, P;Palmer, S;Huang, CS;Clinical evaluation of IntelliPlexTM HCV genotyping kit for hepatitis C virus genotypingDiagn. Microbiol. Infect. Dis.30975461as certified by the commercial supplier in the accompanying Certificate of Analysis (CoA)), including genotypes 3, 4 and 5 were spiked into the sample pool (samples were obtained from SeraCare Life Sciences Inc., [Cambridge, MA United States]; DiscovFull Paper
2019Lagatie, O;Verheyen, A;Nijs, E;Batsa Debrah, L;Debrah, YA;Stuyver, LJ;Performance evaluation of 3 serodiagnostic peptide epitopes and the derived multi-epitope peptide OvNMP-48 for detection of Onchocerca volvulus infectionParasitol. Res.31089811and informed consent, which was signed by all subjects. For samples obtained from Tissue Solutions Ltd. (Glasgow, Scotland), Discovery Life Sciences, Inc. (Los Osos, USA), Bioreclamation IVT (Baltimore, USA), Mayo ClinicFull Paper
2019Montazeri, M;Marbut, C;Huitink, D;Interconnect Fatigue Failure Parameter Isolation for Power Device Reliability Prediction in Alternative Accelerated Mechanical Cycling TestJournal of Electronic PackagingIEEE Forth Workshop on Wide Bandgap Power Devices and Applications ( WiPDA), Fayetteville, AR, Nov. 7-9, pp. 194– 199. 19. Qualitek, 2014, “Technical Data Sheet: Sn42/Bi58 Solder Wire,” Qualtek Electronics Corporation, Addison, IL, Accessed MaFull Paper
2019Mohan, S;Lawton, R;Palmer, C;Rojas, AC;Competitive ELISA method for novel estrogen-negative breast cancer biomarker quantitationJ. Immunol. Methods31533022system) (Rakha et al., 2008), and categorized based on estrogen-hormone-receptor orientation (viz., ER − versus ER + , n = 50), were obtained from cooperative human tissue network (Philadelphia, PA), Tissue-for-Research (Atlanta, GA), and ConversantFull Paper
2019Nicol, AF;de Andrade, CV;Gomes, SC;Brusadelli, MG;Lodin, HM;Wells, SI;Nuovo, GJ;The distribution of novel biomarkers in carcinoma-in-situ, microinvasive, and squamous cell carcinoma of the uterine cervixAnn Diagn Pathol30579259National Institute of Infectious Diseases Evandro Chagas - INI-Fiocruz, Rio de Janeiro, Brazil.++National Institute of Health of Women, Children, and Adolescents, Fernandes Figueira - IFF-FIOCRUZ, Rio de Janeiro, Brazil.++National Institute of HealthFull Paper
2019Mimitou, EP;Cheng, A;Montalbano, A;Hao, S;Stoeckius, M;Legut, M;Roush, T;Herrera, A;Papalexi, E;Ouyang, Z;Satija, R;Sanjana, NE;Koralov, SB;Smibert, P;Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cellsNature methods31011186FBS 10% DMSO and aliquots of 1-1.5 million cells were thawed on the day of the experiment. PBMCs were obtained cryopreserved from AllCells (USA) and used immediately after thawing. NIH-3T3 and HEK293FT cells expressing non-targeting sgRNAs were maintFull Paper
2019Symeonides, S;Choice of Law in the American Courts in 2018: Thirty-Second Annual SurveyThe American Journal of Comparative LawIn re EP Floors Corp., No. 14-18-00610-CV, 2018 WL 4354688 (Tex. App. Sept. 13, 2018); In re Rosewood Private Invs., Inc., No. 05-18-00166-CV, 2018 WL 4403749 (Tex. App. Sept. 17, 2018); Bundy v. Adesa Houston D/B/A Adesa, Inc., No. 01-17-00863-CV, 2Full Paper
2019Yant, SR;Mulato, A;Hansen, D;Tse, WC;Niedziela-Majka, A;Zhang, JR;Stepan, GJ;Jin, D;Wong, MH;Perreira, JM;Singer, E;Papalia, GA;Hu, EY;Zheng, J;Lu, B;Schroeder, SD;Chou, K;Ahmadyar, S;Liclican, A;Yu, H;Novikov, N;Paoli, E;Gonik, D;Ram, RR;Hung, M;McDougall, WM;Brass, AL;Sundquist, WI;Cihlar, T;Link, JO;A highly potent long-acting small-molecule HIV-1 capsid inhibitor with efficacy in a humanized mouse modelNature medicine31501601Human PBMCs were collected from consenting healthy volunteers under informed consent; their use was approved by an institutional review board at AllCells. Human PBMCs, CD4 + T SaveFull Paper
2019Qu, L;Yi, Z;Zhu, S;Wang, C;Cao, Z;Zhou, Z;Yuan, P;Yu, Y;Tian, F;Liu, Z;Bao, Y;Zhao, Y;Wei, W;Programmable RNA editing by recruiting endogenous ADAR using engineered RNAsNature biotechnology3130854010% FBS and IL2 (1,000 U ml-1) and stimulated with CD3/CD28 DynaBeads (ThermoFisher, 11131D) for 2 d. Leukapheresis products from healthy donors were acquired from AllCells LLC China. All healthy donors provided informed consentFull Paper
2019Granja, JM;Klemm, S;McGinnis, LM;Kathiria, AS;Mezger, A;Corces, MR;Parks, B;Gars, E;Liedtke, M;Zheng, GXY;Chang, HY;Majeti, R;Greenleaf, WJ;Single-cell multiomic analysis identifies regulatory programs in mixed-phenotype acute leukemiaNature biotechnology31792411Description of healthy donors. PBMCs, BMMCs and CD34+ bone marrow cells were obtained from healthy donors with informed consent and compliance with relevant ethical regulations (AllCells). Individual information for each donor is provided in SupplemeFull Paper
2019Satpathy, AT;Granja, JM;Yost, KE;Qi, Y;Meschi, F;McDermott, GP;Olsen, BN;Mumbach, MR;Pierce, SE;Corces, MR;Shah, P;Bell, JC;Jhutty, D;Nemec, CM;Wang, J;Wang, L;Yin, Y;Giresi, PG;Chang, ALS;Zheng, GXY;Greenleaf, WJ;Chang, HY;Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustionNature biotechnology31375813obtained from AllCells or the AllCells and isolated per manufacturer recommendations (as outlined in Supplementary Table 4). FACS-isolated populations were obtained from AllCells SaveFull Paper
2019Miller, JC;Patil, DP;Xia, DF;Paine, CB;Fauser, F;Richards, HW;Shivak, DA;Bendaña, YR;Hinkley, SJ;Scarlott, NA;Lam, SC;Reik, A;Zhou, Y;Paschon, DE;Li, P;Wangzor, T;Lee, G;Zhang, L;Rebar, EJ;Enhancing gene editing specificity by attenuating DNA cleavage kineticsNature biotechnology31359006CD34 + stem and progenitor cells were purified from mobilized peripheral blood leukapheresis products obtained from AllCells. Platelet depletion was performed, CD34 + cells were SaveFull Paper
2019Kinney, MA;Vo, LT;Frame, JM;Barragan, J;Conway, AJ;Li, S;Wong, KK;Collins, JJ;Cahan, P;North, TE;Lauffenburger, DA;Daley, GQ;A systems biology pipeline identifies regulatory networks for stem cell engineeringNature biotechnology31267104Human CD34 + progenitors derived from mobilized peripheral blood (AllCells) were expanded for 4 d in StemSpan SFEM (StemCell Technologies) with the addition of interleukin (IL)-3 ( SaveFull Paper
2019Lareau, CA;Duarte, FM;Chew, JG;Kartha, VK;Burkett, ZD;Kohlway, AS;Pokholok, D;Aryee, MJ;Steemers, FJ;Lebofsky, R;Buenrostro, JD;Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibilityNature biotechnology31235917CD34+ stem/progenitor cells, peripheral blood mononuclear cells (PBMC), and isolated peripheral blood CD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from Allcells ( Save;BMMCs, isolated bone marrow CD34 + stem/progenitor cells, PBMCs, and iFull Paper
2019Setty, M;Kiseliovas, V;Levine, J;Gayoso, A;Mazutis, L;Pe'er, D;Characterization of cell fate probabilities in single-cell data with PalantirNature biotechnology30899105incorporate lineage information to model cell fate decisions. scRNA-seq of CD34+ human bone marrow cells Cryopreserved bone marrow stem/progenitor CD34+ cells from healthy donors were purchased from AllCells, LLC. (Cat. No. ABM022F) and stored in vaFull Paper
2019Shah, NJ;Mao, AS;Shih, TY;Kerr, MD;Sharda, A;Raimondo, TM;Weaver, JC;Vrbanac, VD;Deruaz, M;Tager, AM;Mooney, DJ;Scadden, DT;An injectable bone marrow-like scaffold enhances T cell immunity after hematopoietic stem cell transplantationNature biotechnology30742125t-specific antibodies. We isolated hematopoietic cells (Lin−Sca-1hic-kithi) using a FacsAria cell sorter (BD). Sorted cells were ≥ 95% pure. Human cord-blood derived CD34+ cells were purchased (Allcells) and expanded for seven days using expansion suFull Paper
2019Maun, HR;Jackman, JK;Choy, DF;Loyet, KM;Staton, TL;Jia, G;Dressen, A;Hackney, JA;Bremer, M;Walters, BT;Vij, R;Chen, X;Trivedi, NN;Morando, A;Lipari, MT;Franke, Y;Wu, X;Zhang, J;Liu, J;Wu, P;Chang, D;Orozco, LD;Christensen, E;Wong, M;Corpuz, R;Hang, JQ;Lutman, J;Sukumaran, S;Wu, Y;Ubhayakar, S;Liang, X;Schwartz, LB;Babina, M;Woodruff, PG;Fahy, JV;Ahuja, R;Caughey, GH;Kusi, A;Dennis, MS;Eigenbrot, C;Kirchhofer, D;Austin, CD;Wu, LC;Koerber, JT;Lee, WP;Yaspan, BL;Alatsis, KR;Arron, JR;Lazarus, RA;Yi, T;An Allosteric Anti-tryptase Antibody for the Treatment of Mast Cell-Mediated Severe AsthmaCell31585081Enriched CD34+ human cord blood stem cells were obtained from AllCells with more than 80% purity validated by flow cytometry analysis. CHO DKO cell line (Macaraeg et al., 2013) was a derivative of the CHO-K1 cell line (ATCC number CCL-61) was grown aFull Paper
2019Stuart, T;Butler, A;Hoffman, P;Hafemeister, C;Papalexi, E;Mauck, WM;Hao, Y;Stoeckius, M;Smibert, P;Satija, R;Comprehensive Integration of Single-Cell DataCell31178118Hashtag 9 Cell Hashing antibody BioLegend Cat# 394617 Hashtag 10 Cell Hashing antibody BioLegend Cat# 394619 Biological Samples Human bone marrow cells AllCells Cat# ABM007F, lot 3008803 Critical Commercial Assays 3′ scRNA-seq kit 10x Genomics V2 cheFull Paper
2019Abelin, JG;Harjanto, D;Malloy, M;Suri, P;Colson, T;Goulding, SP;Creech, AL;Serrano, LR;Nasir, G;Nasrullah, Y;McGann, CD;Velez, D;Ting, YS;Poran, A;Rothenberg, DA;Chhangawala, S;Rubinsteyn, A;Hammerbacher, J;Gaynor, RB;Fritsch, EF;Greshock, J;Oslund, RC;Barthelme, D;Addona, TA;Arieta, CM;Rooney, MS;Defining HLA-II Ligand Processing and Binding Rules with Mass Spectrometry Enhances Cancer Epitope PredictionImmunity31495665Human Healthy Donor Leukapheresis Material AllCells NA Human Healthy Donor Leukapheresis Material PPA Research NA Human Healthy Donor Leukapheresis Material StemExpress NA Human Healthy Donor Leukapheresis Material StemCell Technologies NAFull Paper
2019Lindner, JM;Cornacchione, V;Sathe, A;Be, C;Srinivas, H;Riquet, E;Leber, XC;Hein, A;Wrobel, MB;Scharenberg, M;Pietzonka, T;Wiesmann, C;Abend, J;Traggiai, E;Human Memory B Cells Harbor Diverse Cross-Neutralizing Antibodies against BK and JC PolyomavirusesImmunity30824324Purified peripheral blood mononuclear cells from leukapharesis AllCells, Inc. N/A leukapharesis and matched serum samples were obtained from AllCells LLC (Alameda, CA, USA). SaveFull Paper
2019Li, R;Wang, Y;Zhang, X;Feng, M;Ma, J;Li, J;Yang, X;Fang, F;Xia, Q;Zhang, Z;Shang, M;Jiang, S;Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasisMolecular cancer30704479HUVECs were purchased from ATCC and cultivated in endothelial cell complete medium containing endothelial cell growth supplement (Allcells, USA). For hypoxic culture, HCC cells SaveFull Paper
2019Jiang, Q;Isquith, J;Zipeto, MA;Diep, RH;Pham, J;Delos Santos, N;Reynoso, E;Chau, J;Leu, H;Lazzari, E;Melese, E;Ma, W;Fang, R;Minden, M;Morris, S;Ren, B;Pineda, G;Holm, F;Jamieson, C;Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor PropagationCancer cell30612940Institutional Review Board approved protocol (#131550) that meets the requirements as stated in 45 CFR 46.404 and 21 CFR 50.51. De-identified (IRB exempt) human cord blood and normal aged-match samples were purchased as purified CD34+ cells from AllCFull Paper
2019Adelman, ER;Huang, HT;Roisman, A;Olsson, A;Colaprico, A;Qin, T;Lindsley, RC;Bejar, R;Salomonis, N;Grimes, HL;Figueroa, ME;Aging Human Hematopoietic Stem Cells Manifest Profound Epigenetic Reprogramming of Enhancers That May Predispose to LeukemiaCancer discovery31085557AML, which is more frequent in the elderly, is characterized by epigenetic deregulation. We demonstrate that epigenetic reprogramming of human HSCs occurs with age, affecting SaveFull Paper
2019Gong, X;Du, J;Parsons, SH;Merzoug, FF;Webster, Y;Iversen, PW;Chio, LC;Van Horn, RD;Lin, X;Blosser, W;Han, B;Jin, S;Yao, S;Bian, H;Ficklin, C;Fan, L;Kapoor, A;Antonysamy, S;Mc Nulty, AM;Froning, K;Manglicmot, D;Pustilnik, A;Weichert, K;Wasserman, SR;Dowless, M;Marugán, C;Baquero, C;Lallena, MJ;Eastman, SW;Hui, YH;Dieter, MZ;Doman, T;Chu, S;Qian, HR;Ye, XS;Barda, DA;Plowman, GD;Reinhard, C;Campbell, RM;Henry, JR;Buchanan, SG;Aurora A Kinase Inhibition Is Synthetic Lethal with Loss of the RB1 Tumor Suppressor GeneCancer discovery30373917The identification of a synthetic lethal interaction between RB1 and AURKA inhibition, and the discovery of a drug that can be dosed continuously to achieve uninterrupted inhibition of SaveFull Paper
2019Mascarenhas, J;Lu, M;Kosiorek, H;Virtgaym, E;Xia, L;Sandy, L;Mesa, R;Petersen, B;Farnoud, N;Najfeld, V;Rampal, R;Dueck, A;Hoffman, R;Oral idasanutlin in patients with polycythemia veraBlood31167802Baseline MDM2 protein levels in the enrolled PV/ET patients and 7 normal samples (peripheral blood, n = 4; bone marrow, n = 3) purchased from ALLcells (Alameda, CA) were evaluated using monoclonal antibody staining and flow cytometric analysis.24 BriFull Paper
2019Drissen, R;Thongjuea, S;Theilgaard-Mönch, K;Nerlov, C;Identification of two distinct pathways of human myelopoiesisScience immunology31126997Bone marrow samples were from AllCells or taken from healthy male volunteers at the age of 21 to 29 years old, who provided written informed consent in accordance with local SaveFull Paper
2019Hu, CW;Qiu, Y;Ligeralde, A;Raybon, AY;Yoo, SY;Coombes, KR;Qutub, AA;Kornblau, SM;A quantitative analysis of heterogeneities and hallmarks in acute myelogenous leukaemiaNature biomedical engineering30988472was prepared as described in publications11,21. In addition to AML patient samples, each array includes the same ten cryopreserved normal bone marrow CD34+ samples (NLBM CD34+) from healthy subjects (AllCells, Alameda, CA). Mutation Analysis All mutFull Paper
2019Maekawa, T;Kato, S;Kawamura, T;Takada, K;Sone, T;Ogata, H;Saito, K;Izumi, T;Nagao, S;Takano, K;Okada, Y;Tachi, N;Teramoto, M;Horiuchi, T;Hikota-Saga, R;Endo-Umeda, K;Uno, S;Osawa, Y;Kobayashi, A;Kobayashi, S;Sato, K;Hashimoto, M;Suzu, S;Usuki, K;Morishita, S;Araki, M;Makishima, M;Komatsu, N;Kimura, F;Increased SLAMF7high monocytes in myelofibrosis patients harboring JAK2V617F provide a therapeutic target of elotuzumabBlood31270105The hNOG mouse was created by transplanting human cord blood (Allcells, Alameda, CA) into the NOG mouse established by backcrossing an interleukin-2R (IL-2R) γ-chain knockout SaveFull Paper
2019Pang, WW;Czechowicz, A;Logan, AC;Bhardwaj, R;Poyser, J;Park, CY;Weissman, IL;Shizuru, JA;Anti-CD117 antibody depletes normal and myelodysplastic syndrome human hematopoietic stem cells in xenografted miceBlood30745302Normal human BM samples were purchased from AllCells (Alameda, CA). Human umbilical cord blood (UCB) samples and human BM samples from MDS patients were obtained SaveFull Paper
2019Abdel-Azim, H;Dvorak, C;Bunin, N;Walters, M;Talano, J;Anderson, E;Malvar, J;Al Jassani, M;Chaudhury, S;Cairo, M;Kitko, C;Jacob, A;Monos, D;Wing, L;Pulsipher, M;Next Generation Sequence Minimal Residual Disease (NGS-MRD) Predicts Outstanding Event Free Survival (EFS) Regardless of Hematopoietic Cell Transplantation (HCT) Preparative Approach or Graft Alpha/Beta Depletion in Children with Acute Lymphoblastic Leukemia (ALL)BloodMRD detected by flow cytometry (FC) or PCR has been associated with key outcomes after HCT for ALL. In a prospective multicenter trial (NCT02646839; Pediatric Blood and Marrow Transplant Consortium [PBMTC] ONC1401), we performed a planned sub-study oFull Paper
2019Dunbar, A;Kosiorek, H;Krishnan, A;McGovern, E;Park, Y;Patel, M;Weinberg, R;Yacoub, A;Dueck, A;Mascarenhas, J;Levine, R;Hoffman, R;Rampal, R;Identifying Cytokine Biomarkers of Response to Pegylated-Interferon Therapy in Polycythemia Vera and Essential Thrombocythemia: Correlative Analysis from the MPN-RC 111/112 TrialsBloodAge-matched serum from healthy individuals (N=6) was obtained from AllCells TM for use as wild-type (WT) controls. Baseline cytokine samples were compared between groups of Save;Background: Pegylated Interferon-alpha (PEG-IFNa) improves hematologicFull Paper
2019Park, SH;Lee, CM;Dever, DP;Davis, TH;Camarena, J;Srifa, W;Zhang, Y;Paikari, A;Chang, AK;Porteus, MH;Sheehan, VA;Bao, G;Highly efficient editing of the β-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell diseaseNucleic acids research31147717K562 cells were cultured in RPMI1640 (ATCC, Cat No. 30-2001) supplemented with 10% FBS and L-glutamine. Frozen CD34+ cells from mobilized peripheral blood of normal individuals were purchased from AllCells. Peripheral blood CD34+ cells were acquiredFull Paper
2019Shortridge, MD;Wille, PT;Jones, AN;Davidson, A;Bogdanovic, J;Arts, E;Karn, J;Robinson, JA;Varani, G;An ultra-high affinity ligand of HIV-1 TAR reveals the RNA structure recognized by P-TEFbNucleic acids research30481318stimulate HIV-1 transcription. Twenty-four hours later, the percentage of cells expressing GFP was determined by flow cytometry as described above. Peptide uptake Frozen, de-identified human PBMCs (Allcells, Alameda, CA) were thawed and stimulated wFull Paper
2019McGrath, E;Shin, H;Zhang, L;Phue, JN;Wu, WW;Shen, RF;Jang, YY;Revollo, J;Ye, Z;Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencingNature communications31767844Briefly, CD34+ cells from a healthy male donor were purchased from AllCells (Alameda, CA, USA) and were cultured in StemSpan medium (STEMCELL Technologies, Vancouver, BC, SaveFull Paper
2019Sachdeva, M;Busser, BW;Temburni, S;Jahangiri, B;Gautron, AS;Maréchal, A;Juillerat, A;Williams, A;Depil, S;Duchateau, P;Poirot, L;Valton, J;Repurposing endogenous immune pathways to tailor and control chimeric antigen receptor T cell functionalityNature communications31723132are able to integrate complex environmental inputs and to generate custom outputs in a highly regulated and specific manner. Methods Biological materials Cryopreserved human PBMCs were acquired from ALLCELLS (cat # PB006F) and used in accordance witFull Paper
2019Gomez-Ospina, N;Scharenberg, SG;Mostrel, N;Bak, RO;Mantri, S;Quadros, RM;Gurumurthy, CB;Lee, C;Bao, G;Suarez, CJ;Khan, S;Sawamoto, K;Tomatsu, S;Raj, N;Attardi, LD;Aurelian, L;Porteus, MH;Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type INature communications31492863ck-treated samples, and 50% modification control were included. Data was analyzed using QuantaSoft (Bio-Rad). HSPC selection and culturing Human CD34+ HSPCs mobilized peripheral blood purchased from AllCells (Alameda, CA, USA) and thawed per manufacFull Paper
2019Zhang, Y;Roos, M;Himburg, H;Termini, CM;Quarmyne, M;Li, M;Zhao, L;Kan, J;Fang, T;Yan, X;Pohl, K;Diers, E;Jin Gim, H;Damoiseaux, R;Whitelegge, J;McBride, W;Jung, ME;Chute, JP;PTPσ inhibitors promote hematopoietic stem cell regenerationNature communications31413255collected from the recipient mice and were analyzed for percentages of DsRed+ cells in the BM17. Human BM cultures and human BM transplantation assays Human BM mononuclear cells were purchased from AllCells. Cryopreserved human BM cells were recoverFull Paper
2019Pellin, D;Loperfido, M;Baricordi, C;Wolock, SL;Montepeloso, A;Weinberg, OK;Biffi, A;Klein, AM;Biasco, L;A comprehensive single cell transcriptional landscape of human hematopoietic progenitorsNature communications31160568from MNCs with the human anti-CD34 MicroBeads Isolation Kit (Miltenyi Biotec) according to the manufacturer's specifications or were purchased from commercial sources (AllCells). SaveFull Paper
2019Pavel-Dinu, M;Wiebking, V;Dejene, BT;Srifa, W;Mantri, S;Nicolas, CE;Lee, C;Bao, G;Kildebeck, EJ;Punjya, N;Sindhu, C;Inlay, MA;Saxena, N;DeRavin, SS;Malech, H;Roncarolo, MG;Weinberg, KI;Porteus, MH;Gene correction for SCID-X1 in long-term hematopoietic stem cellsNature communications30967552d to the purified AAV6 at a final concentration of 0.001%, aliquot and stored at −80 °C. CD34+ HSPCs Mobilized peripheral blood  (mPB) and bone marow (BM) CD34+ HSPCs cells were purchased from AllCells (Alameda, CA, USA). Cells were thawed using pubFull Paper
2019Kimmey, SC;Borges, L;Baskar, R;Bendall, SC;Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cellsNature communications30862852Fresh bone marrow aspirates from two independent donors were obtained from AllCells (Alameda, CA) on the same day of collection, and immediately after receiving was placed in 37 SaveFull Paper
2019Paschon, DE;Lussier, S;Wangzor, T;Xia, DF;Li, PW;Hinkley, SJ;Scarlott, NA;Lam, SC;Waite, AJ;Truong, LN;Gandhi, N;Kadam, BN;Patil, DP;Shivak, DA;Lee, GK;Holmes, MC;Zhang, L;Miller, JC;Rebar, EJ;Diversifying the structure of zinc finger nucleases for high-precision genome editingNature communications30850604rer’s instructions. Target loci were then analyzed by PCR amplification followed by deep sequencing (MiSeq). Studies performed in T-cells Peripheral blood CD3 + Pan T-cells were purchased from AllCells (Alameda, CA). Transfections into activated T-cFull Paper
2019Czechowicz, A;Palchaudhuri, R;Scheck, A;Hu, Y;Hoggatt, J;Saez, B;Pang, WW;Mansour, MK;Tate, TA;Chan, YY;Walck, E;Wernig, G;Shizuru, JA;Winau, F;Scadden, DT;Rossi, DJ;Selective hematopoietic stem cell ablation using CD117-antibody-drug-conjugates enables safe and effective transplantation with immunity preservationNature communications30728354als into 9 Gy, lethally irradiated, C57BL/6J (CD45.2+) wild-type recipients as previously described16. Human cord blood transplantation Single donor-derived cord blood CD34+ cells were obtained from AllCells (Alameda, CA) under local IRB-approved prFull Paper
2019Zeng, Y;Yao, X;Liu, X;He, X;Li, L;Liu, X;Yan, Z;Wu, J;Fu, BM;Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesisJournal of extracellular vesicles31258881o inhibit tumor vasculogenesis may serve as a novel strategy to augment the long-term efficacy of anti-angiogenic and anti-autophagic therapies for tumors. Materials and methods Cell culture HUVECs (Allcells, Shanghai, China) were cultured in HUVECFull Paper
2019Mu, Q;Cabana-Puig, X;Mao, J;Swartwout, B;Abdelhamid, L;Cecere, TE;Wang, H;Reilly, CM;Luo, XM;Pregnancy and lactation interfere with the response of autoimmunity to modulation of gut microbiotaMicrobiome31311609Human plasma samples were purchased from AllCells (Alameda, CA). For measuring anti-DNA IgG, anti-LPS IgG, and anti-flagellin IgG, high-binding plates were coated overnight at 4 SaveFull Paper
2019Li, W;Wu, D;Zhu, S;Liu, Z;Luo, B;Lu, L;Zhou, C;Sustained release of plasmid DNA from PLLA/POSS nanofibers for angiogenic therapyChemical Engineering JournalHuman umbilical vein endothelial cells (HUVECs, purchased from Allcells, shanghai, China) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (P/S, Gibco) at 37 °C in 5% CO2Full Paper
2019Wu, HW;Sheard, MA;Malvar, J;Fernandez, GE;DeClerck, YA;Blavier, L;Shimada, H;Theuer, CP;Sposto, R;Seeger, RC;Anti-CD105 Antibody Eliminates Tumor Microenvironment Cells and Enhances Anti-GD2 Antibody Immunotherapy of Neuroblastoma with Activated Natural Killer CellsClinical cancer research : an official journal of the American Association for Cancer Research31068371Human umbilical vein endothelial cells (HUVEC) were purchased from AllCells, LLC and maintained in HUVEC Basal Medium containing 10% Stimulatory Supplement from AllCells in SaveFull Paper
2019Zuch de Zafra, CL;Fajardo, F;Zhong, W;Bernett, MJ;Muchhal, US;Moore, GL;Stevens, J;Case, R;Pearson, JT;Liu, S;McElroy, PL;Canon, J;Desjarlais, JR;Coxon, A;Balazs, M;Nolan-Stevaux, O;Targeting Multiple Myeloma with AMG 424, a Novel Anti-CD38/CD3 Bispecific T-cell-recruiting Antibody Optimized for Cytotoxicity and Cytokine ReleaseClinical cancer research : an official journal of the American Association for Cancer Research30918018Frozen human T cells (AllCells) or frozen peripheral blood mononuclear cells (PBMC) from humans or cynomolgus (CepheusBio) were cocultured with target cells at an effector cell (E) SaveFull Paper
2019Jin, Q;Martinez, CA;Arcipowski, KM;Zhu, Y;Gutierrez-Diaz, BT;Wang, KK;Johnson, MR;Volk, AG;Wang, F;Wu, J;Grove, C;Wang, H;Sokirniy, I;Thomas, PM;Goo, YA;Abshiru, NA;Hijiya, N;Peirs, S;Vandamme, N;Berx, G;Goosens, S;Marshall, SA;Rendleman, EJ;Takahashi, YH;Wang, L;Rawat, R;Bartom, ET;Collings, CK;Van Vlierberghe, P;Strikoudis, A;Kelly, S;Ueberheide, B;Mantis, C;Kandela, I;Bourquin, JP;Bornhauser, B;Serafin, V;Bresolin, S;Paganin, M;Accordi, B;Basso, G;Kelleher, NL;Weinstock, J;Kumar, S;Crispino, JD;Shilatifard, A;Ntziachristos, P;USP7 Cooperates with NOTCH1 to Drive the Oncogenic Transcriptional Program in T-Cell LeukemiaClinical cancer research : an official journal of the American Association for Cancer Research30224337were maintained in DMEM medium supplemented with 10% heat-inactivated FBS, 2% penicillin/streptomycin, and 1% GlutaMAX. Human pan T cells were purchased from AllCells.com (Alameda, CA). Primary human samples were collected by collaborating institutioFull Paper
2019Zheng, B;Yu, SF;Del Rosario, G;Leong, SR;Lee, GY;Vij, R;Chiu, C;Liang, WC;Wu, Y;Chalouni, C;Sadowsky, J;Clark, V;Hendricks, A;Poon, KA;Chu, W;Pillow, T;Schutten, MM;Flygare, J;Polson, AG;An Anti-CLL-1 Antibody-Drug Conjugate for the Treatment of Acute Myeloid LeukemiaClinical cancer research : an official journal of the American Association for Cancer Research29959143Bone marrow mononuclear cells (BM-MNC) from healthy human donors (AllCells) were prepared by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare). CD34 + SaveFull Paper
2019Castillo, J;Wu, E;Lowe, C;Srinivasan, S;McCord, R;Wagle, MC;Jayakar, S;Edick, MG;Eastham-Anderson, J;Liu, B;Hutchinson, KE;Jones, W;Stokes, MP;Tarighat, SS;Holcomb, T;Glibicky, A;Romero, FA;Magnuson, S;Huang, SA;Plaks, V;Giltnane, JM;Lackner, MR;Mounir, Z;CBP/p300 Drives the Differentiation of Regulatory T Cells through Transcriptional and Non-Transcriptional MechanismsCancer research31182547Normal human peripheral blood CD4+/CD45RA+/CD25− naïve T cells were procured from Allcells (PB009-5). Cells were isolated using Immunomagnetic CD4+ Isolation Kit, CD45RO microbeads to deplete CD45RO+ cells, and cultured using Dynabeads human T activaFull Paper
2019Levi, J;Lam, T;Goth, SR;Yaghoubi, S;Bates, J;Ren, G;Jivan, S;Huynh, TL;Blecha, JE;Khattri, R;Schmidt, KF;Jennings, D;VanBrocklin, H;Imaging of Activated T Cells as an Early Predictor of Immune Response to Anti-PD-1 TherapyCancer research31064845Neutrophils and eosinophils were isolated from human whole blood (AllCells, Alameda CA). Human neutrophil and eosinophil enrichment kit (StemCell Technologies) was used to purify the cells. Neutrophils were either treated with 1uM N-formyl- L-methionFull Paper
2019Boudreaux, SP;Duren, RP;Call, SG;Nguyen, L;Freire, PR;Narayanan, P;Redell, MS;Conneely, OM;Drug targeting of NR4A nuclear receptors for treatment of acute myeloid leukemiaLeukemia29884904rmed consent at Texas Children’s Hospital in accordance with the Declaration of Helsinki and institutional review board regulations. Normal cord blood CD34+ stem/progenitor cells were obtained from AllCells. Connectivity Map (CMAP) Analysis The NR4AFull Paper
2019Shen, JC;Kamath-Loeb, AS;Kohrn, BF;Loeb, KR;Preston, BD;Loeb, LA;A high-resolution landscape of mutations in the BCL6 super-enhancer in normal human B cellsProceedings of the National Academy of Sciences of the United States of America31748270Human B Cells. The CD19+ B cells were purchased from AllCells and ReachBio. Peripheral blood mononuclear cells (PBMCs) were purified from whole blood drawn from 10 healthy donors (SI Appendix, Table S2). CD19+ B cells were purified by positive selectFull Paper
2019Zhang, L;Dong, X;Lee, M;Maslov, AY;Wang, T;Vijg, J;Single-cell whole-genome sequencing reveals the functional landscape of somatic mutations in B lymphocytes across the human lifespanProceedings of the National Academy of Sciences of the United States of America30992375Two frozen human umbilical cord blood mononuclear cell samples were obtained from AllCells and STEMCELL Technologies. Informed consent was obtained from all the donors. The SaveFull Paper
2019Ye, S;Cohen, D;Belmar, NA;Choi, D;Tan, SS;Sho, M;Akamatsu, Y;Kim, H;Iyer, R;Cabel, J;Lake, M;Song, D;Harlan, J;Zhang, C;Fang, Y;Wahl, AF;Culp, P;Hollenbaugh, D;Chao, DT;A Bispecific Molecule Targeting CD40 and Tumor Antigen Mesothelin Enhances Tumor-Specific ImmunityCancer immunology research31462409than 10 passages from master cell banking. Fresh human peripheral blood mononuclear cells (PBMC) from healthy donors (about 109 per donor) were purchased from AllCells. Donor virus testing results of human immunodeficiency virus, hepatitis B virus, aFull Paper
2019Gui, X;Deng, M;Song, H;Chen, Y;Xie, J;Li, Z;He, L;Huang, F;Xu, Y;Anami, Y;Yu, H;Yu, C;Li, L;Yuan, Z;Xu, X;Wang, Q;Chai, Y;Huang, T;Shi, Y;Tsuchikama, K;Liao, XC;Xia, N;Gao, GF;Zhang, N;Zhang, CC;An, Z;Disrupting LILRB4/APOE Interaction by an Efficacious Humanized Antibody Reverses T-cell Suppression and Blocks AML DevelopmentCancer immunology research31213474In the coculture assay, human T cells (5 × 10 4 cells per well) isolated from health donor peripheral blood (AllCells) were placed in the lower chambers of a 96-well transwell plate. SaveFull Paper
2019Newman, M;Abstract B178: Development and preclinical efficacy characterization of a systemically administered multiple Toll-like receptor (TLR) agonist for antitumor immunotherapyCancer Immunology ResearchPBMCs from fresh normal peripheral blood leukapheresis paks (ALLCells, Alameda, CA) were isolated using a Ficoll gradient. Ten-fold increments of 1x10/mL to 1x10^8/mL untreated SaveFull Paper
2019Sun, Y;Xiao, Y;Sun, H;Zhao, Z;Zhu, J;Zhang, L;Dong, J;Han, T;Jing, Q;Zhou, J;Jing, Z;miR-27a regulates vascular remodeling by targeting endothelial cells' apoptosis and interaction with vascular smooth muscle cells in aortic dissectionTheranostics31695809Prime HUVECs were purchased from ALLCELLS and cultured in endothelial cell media (ALLCELLS, Emeryville, California) at 37℃ in a humidified atmosphere with 5% CO 2 . HEK 293 SaveFull Paper
2019Varricchio, L;Planutis, A;Manwani, D;Jaffray, J;Mitchell, WB;Migliaccio, AR;Bieker, JJ;Genetic disarray follows mutant KLF1-E325K expression in a congenital dyserythropoietic anemia patientHaematologica30872368no new patient material was obtained for the present study. Mononuclear cells from the peripheral blood (PBMC) had been isolated and cryopreserved as described.35 Non-patient PBMC were purchased from AllCells (PB003F). Human erythroid massive amplifFull Paper
2019Sapienza, MR;Abate, F;Melle, F;Orecchioni, S;Fuligni, F;Etebari, M;Tabanelli, V;Laginestra, MA;Pileri, A;Motta, G;Rossi, M;Agostinelli, C;Sabattini, E;Pimpinelli, N;Truni, M;Falini, B;Cerroni, L;Talarico, G;Piccioni, R;Amente, S;Indio, V;Tarantino, G;Brundu, F;Paulli, M;Berti, E;Facchetti, F;Dellino, GI;Bertolini, F;Tripodo, C;Rabadan, R;Pileri, SA;Blastic plasmacytoid dendritic cell neoplasm: genomics mark epigenetic dysregulation as a primary therapeutic targetHaematologica30381297RNA of 4 normal plasmacytoid dendritic cell (pDCs) samples was purchased from AllCells (Alameda, CA, US) and used for comparison. For details, see Online Supplementary Table S6 Save;BPDCN cryopreserved cutaneous biopsies, sufficient only for RNA seFull Paper
2019Yao, X;Liu, R;Liang, X;Ding, J;Critical Areas of Proliferation of Single Cells on Micropatterned Surfaces and Corresponding Cell Type DependenceACS applied materials & interfaces30964630Primary rat MSCs were purchased from Allcells Tech., Shanghai, People’s Republic of China; primary mouse embryo fibroblasts (EF) were purchased from Shanghai Gaining Biological Technology Co., Ltd.; human cervix epithelial carcinoma cells (Hela) andFull Paper
2019Deng, X;Chu, X;Wang, P;Ma, X;Wei, C;Sun, C;Yang, J;Li, Y;MicroRNA-29a-3p Reduces TNFα-Induced Endothelial Dysfunction by Targeting Tumor Necrosis Factor Receptor 1Molecular therapy. Nucleic acids31760375ina), and HEK293 cells (ATCC, Manassas, VA, USA) were maintained in DMEM (HyClone, South Logan, UT, USA) containing 10% fetal bovine serum (FBS; PAA Laboratories, Austria). pHUVECs were obtained from AllCells (China) and cultured incomplete medium (AFull Paper
2019Mandal, S;Prathipati, PK;Belshan, M;Destache, CJ;A potential long-acting bictegravir loaded nano-drug delivery system for HIV-1 infection: A proof-of-concept studyAntiviral research30991088TZM-bl cells were obtained from NIH AIDS reagent program (Kappes and Wu, 2018; Kappes et al., 2015). Peripheral blood mononuclear cells (PBMCs) were obtained from commercial vendor (AllCells Inc. Alameda, CA). TZM-bl cells were maintained in completeFull Paper
2019Bongen, E;Lucian, H;Khatri, A;Fragiadakis, GK;Bjornson, ZB;Nolan, GP;Utz, PJ;Khatri, P;Sex Differences in the Blood Transcriptome Identify Robust Changes in Immune Cell Proportions with Aging and Influenza InfectionCell reports31722210Sample preparation and CyTOF methods have been described previously (Fragiadakis et al., 2019). Briefly, peripheral blood was obtained from 86 human adult volunteers from the Stanford Blood Center, AllCells (exempt, non-human subjects research), andFull Paper
2019Shehata, L;Maurer, DP;Wec, AZ;Lilov, A;Champney, E;Sun, T;Archambault, K;Burnina, I;Lynaugh, H;Zhi, X;Xu, Y;Walker, LM;Affinity Maturation Enhances Antibody Specificity but Compromises Conformational StabilityCell reports31553901Biological Samples Healthy donor bone marrow mononuclear cells AllCells https://www.allcells.com/ Healthy human peripheral blood mononuclear cells Dartmouth Hitchcock Medical Center N/AFull Paper
2019Shi, D;Dong, Y;Dai, P;Gao, J;Yin, J;Xie, M;Dentin matrix protein 1 correlates with the severity of hemorrhagic fever with renal syndrome and promotes hyper-permeability of endothelial cells infected by Hantaan virusMicrobes and infection30735719Cells were cultured in AllCells complete endothelial cell medium and incubated at 37 C with 5% CO 2 . HUVECs (passage 3 or 4) of approximately 70-80% confluence were used for all SaveFull Paper
2019Muñoz, DP;Yannone, SM;Daemen, A;Sun, Y;Vakar-Lopez, F;Kawahara, M;Freund, AM;Rodier, F;Wu, JD;Desprez, PY;Raulet, DH;Nelson, PS;van 't Veer, LJ;Campisi, J;Coppé, JP;Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to agingJCI insight31184599Freshly isolated human PBMCs were maintained in supplemented RPMI media and cultured in 3% O2 conditions. Monocytes were obtained from ATCC (U934 and Thp1) or AllCells (CD36 selection; 99%pure). Purified NK, T, and DC cells were from AllCells (99%purFull Paper
2019Morgan, H;Tseng, SY;Gallais, Y;Leineweber, M;Buchmann, P;Riccardi, S;Nabhan, M;Lo, J;Gani, Z;Szely, N;Zhu, CS;Yang, M;Kiessling, A;Vohr, HW;Pallardy, M;Aswad, F;Turbica, I;Evaluation of in vitro Assays to Assess the Modulation of Dendritic Cells Functions by Therapeutic Antibodies and AggregatesFrontiers in immunology31001248Wuppertal (Germany). For each site, healthy donors gave their written consent for the use of blood donation for research purposes. Also, PBMC directly isolated and frozen were purchased from vendor (AllCells, CA, USA). In each partner lab's, PBMC isFull Paper
2019Saul, L;Mair, I;Ivens, A;Brown, P;Samuel, K;Campbell, JDM;Soong, DY;Kamenjarin, N;Mellanby, RJ;1,25-Dihydroxyvitamin D3 Restrains CD4+ T Cell Priming Ability of CD11c+ Dendritic Cells by Upregulating Expression of CD31Frontiers in immunology30984180SA as per manufacturer's instructions (eBioscience, San Diego, USA). Generation of Human DC From Mobilized Peripheral Blood CD34+ Progenitor Cells CD34+ cells were obtained from a commercial source (AllCells, LLC., Alameda, CA, USA). The CD34+ cellsFull Paper
2019Smith, AJ;Miller, SM;Buhl, C;Child, R;Whitacre, M;Schoener, R;Ettenger, G;Burkhart, D;Ryter, K;Evans, JT;Species-Specific Structural Requirements of Alpha-Branched Trehalose Diester Mincle AgonistsFrontiers in immunology30873180PBMCs were obtained via leukapheresis from normal donors (AllCells, Berkeley, CA) or fresh blood was obtained from health human donors through a University of Montana SaveFull Paper
2019Du, Y;Montoya, C;Orrego, S;Wei, X;Ling, J;Lelkes, PI;Yang, M;Topographic cues of a novel bilayered scaffold modulate dental pulp stem cells differentiation by regulating YAP signalling through cytoskeleton adjustmentsCell proliferation31424140ilometer (Surfcorder SE 1700, Kosaka Labs) with a 2 μm radius tip, a spread of 2.5 mm and a speed of 0.5 mm/s. 2.2 Dental pulp stem cells culture on the scaffolds Human DPSCs were purchased from AllCells LLC. These cells were guaranteed by company tFull Paper
2019Cunin, P;Bouslama, R;Machlus, KR;Martínez-Bonet, M;Lee, PY;Wactor, A;Nelson-Maney, N;Morris, A;Guo, L;Weyrich, A;Sola-Visner, M;Boilard, E;Italiano, JE;Nigrovic, PA;Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to plateletseLife31042146and Schulze, 2005). Fetal liver-derived MKs were generated as described (Machlus et al., 2017). Human MK generation: mobilized peripheral blood or BM CD34 +stem/progenitor cells were purchased from AllCells. 1 × 105 cells were cultured in StemSpan meFull Paper
2019Dura-Bernal, S;Suter, BA;Gleeson, P;Cantarelli, M;Quintana, A;Rodriguez, F;Kedziora, DJ;Chadderdon, GL;Kerr, CC;Neymotin, SA;McDougal, RA;Hines, M;Shepherd, GM;Lytton, WW;NetPyNE, a tool for data-driven multiscale modeling of brain circuitseLife31025934ode to disk file and combines these files after the simulation has ended. After gathering, the built-in analysis functions have direct access to all the network and simulation output data via sim.net.allCells and sim.allSimData. Importing and exportFull Paper
2019Frank, ND;Jones, ME;Vang, B;Coeshott, C;Evaluation of reagents used to coat the hollow-fiber bioreactor membrane of the Quantum® Cell Expansion System for the culture of human mesenchymal stem cellsMaterials science & engineering. C, Materials for biological applications30606590Healthy, fresh, adult human whole bone marrow (WBM) was purchased either from AllCells LLC (Alameda, CA) or from Lonza (Basel, Switzerland). Flow cytometry staining buffer was SaveFull Paper
2019Laner-Plamberger, S;Oeller, M;Mrazek, C;Hartl, A;Sonderegger, A;Rohde, E;Strunk, D;Schallmoser, K;Upregulation of mitotic bookmarking factors during enhanced proliferation of human stromal cells in human platelet lysateJournal of translational medicine31888679ed from white adipose tissue (WAT) and umbilical cord (UC) after signed informed consent by the donors as described previously [26, 30, 31]. Bone marrow (BM)-derived stromal cells were purchased from AllCells, (Alameda, CA, USA, http://www.allcells.cFull Paper
2019Antebi, B;Asher, AM;Rodriguez, LA;Moore, RK;Mohammadipoor, A;Cancio, LC;Cryopreserved mesenchymal stem cells regain functional potency following a 24-h acclimation periodJournal of translational medicine31464641Human MSCs were isolated from fresh bone-marrow mononuclear cells purchased from AllCells (Alameda, CA). The MSCs were expanded in complete culture media (CCM) consisting SaveFull Paper
2019Coeshott, C;Vang, B;Jones, M;Nankervis, B;Large-scale expansion and characterization of CD3+ T-cells in the Quantum® Cell Expansion SystemJournal of translational medicine31391068g and unique dual-loop circuits. Materials and methods Preparation of peripheral blood mononuclear cells (PBMCs) Peripheral blood leukapheresis products were obtained from individual healthy donors (AllCells, LLC, Alameda, CA, HemaCare Corp, Van NuyFull Paper
2019Shi, L;Zhong, Z;Li, X;Zhou, Y;Pan, Z;Discovery of an Orally Available Janus Kinase 3 Selective Covalent InhibitorJournal of medicinal chemistry30615446Frozen PBMCs (from Allcells) were thawed in RPMI1640 (Thermo Fisher) containing 10% FBS and recovered overnight at 37 °C. The next day, cells were diluted to 1 × 106 cells/mL and seeded (500 μL) in 6-well plates. Compounds or DMSO (5 μL, seriallyFull Paper
2019Zoine, JT;Knight, KA;Fleischer, LC;Sutton, KS;Goldsmith, KC;Doering, CB;Spencer, HT;Ex vivo expanded patient-derived γδ T-cell immunotherapy enhances neuroblastoma tumor regression in a murine modelOncoimmunology31413905Commercially available healthy donor frozen PBMCs were obtained from AllCells (Alameda, CA). At the time of stem cell collection, each patient had undergone two cycles of induction SaveFull Paper
2019Rodriguez, LA;Mohammadipoor, A;Alvarado, L;Kamucheka, RM;Asher, AM;Cancio, LC;Antebi, B;Preconditioning in an Inflammatory Milieu Augments the Immunotherapeutic Function of Mesenchymal Stromal CellsCells31096722D) for immune- and/or inflammatory-mediated diseases. 2. Materials and Methods 2.1. Isolation of BM-MSCs and AD-MSCs Human BM-MSCs were isolated from commercially available mononuclear cells (MNCs) (AllCells LLC; Emeryville, CA, USA), as previouslyFull Paper
2019Youn, M;Huang, H;Chen, C;Kam, S;Wilkes, MC;Chae, HD;Sridhar, KJ;Greenberg, PL;Glader, B;Narla, A;Lin, S;Sakamoto, KM;MMP9 inhibition increases erythropoiesis in RPS14-deficient del(5q) MDS models through suppression of TGF-β pathwaysBlood advances31540902Primary human CD34 + hematopoietic stem/progenitor cells were purified from bone marrow aspirates purchased from Allcells and Stemcell Technologies. CD34 + cells were purified SaveFull Paper
2019Li, C;Mathews, V;Kim, S;George, B;Hebert, K;Jiang, H;Li, C;Zhu, Y;Keesler, DA;Boelens, JJ;Dvorak, CC;Agarwal, R;Auletta, JJ;Goyal, RK;Hanna, R;Kasow, K;Shenoy, S;Smith, AR;Walters, MC;Eapen, M;Related and unrelated donor transplantation for β-thalassemia major: results of an international surveyBlood advances31471325Full Paper
2019Yang, HM;Choi, JJ;Kim, HN;Yang, SJ;Park, SJ;Kang, C;Chung, HM;Lee, MR;Kim, SJ;Moon, SH;Reconstituting Human Cutaneous Regeneration in Humanized Mice under Endothelial Cell TherapyThe Journal of investigative dermatology30393080The derivation of CB-EPCs was performed from CB mononuclear cells, which were purchased from AllCells (Alameda, CA). Briefly, CB mononuclear cells were seeded at a density of 4-6 × 106/mL in a collagen-coated tissue culture dish and cultured in EGM-2Full Paper
2019Jeng, MY;Mumbach, MR;Granja, JM;Satpathy, AT;Chang, HY;Chang, ALS;Enhancer Connectome Nominates Target Genes of Inherited Risk Variants from Inflammatory Skin DisordersThe Journal of investigative dermatology30315781Briefly, donor human peripheral blood cells were obtained from AllCells (Emeryville, CA) and were sorted based on the following markers: CD4 + CD25 − CD45RA + (naive), CD4 + SaveFull Paper
2019Naipauer, J;Rosario, S;Gupta, S;Premer, C;Méndez-Solís, O;Schlesinger, M;Ponzinibbio, V;Jain, V;Gay, L;Renne, R;Chan, HL;Morey, L;Salyakina, D;Abba, M;Williams, S;Hare, JM;Goldschmidt-Clermont, PJ;Mesri, EA;PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environmentPLoS pathogens31881074n SCA-1; and (iii) the ability to differentiate into adipocytes or osteoblast-like cells. Human MSCs were isolated as described previously [39]. Briefly, BM aspirates (25-50 mL) were purchased from AllCells (Emeryville, CA) under appropriate informedFull Paper
2019Bi, W;Xu, W;Cheng, L;Xue, J;Wang, Q;Yu, F;Xia, S;Wang, Q;Li, G;Qin, C;Lu, L;Su, L;Jiang, S;IgG Fc-binding motif-conjugated HIV-1 fusion inhibitor exhibits improved potency and in vivo half-life: Potential application in combination with broad neutralizing antibodiesPLoS pathogens31805154Env (2.5 × 104 cells) as the target cells were seeded into a 96-well plate (25 μL/well), and the mixture of a serially diluted inhibitors and 10E8 (1.5 μg/mL) was added to the culture plate. PBMC (AllCells) as effector cells (2.5 × 104 cells in 25 μLFull Paper
2019Wang, W;Liu, T;Yang, L;Ma, Y;Dou, F;Shi, L;Wen, A;Ding, Y;Study on the multi-targets mechanism of triphala on cardio-cerebral vascular diseases based on network pharmacologyBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapie31112872Triphala was obtained from Tibet Jinzhu Along Tibetan Medicine Co. Ltd. (Tibet, China). The Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from AllCells (Emeryville, CA, USA). Primary antibodies against β-actin, PTGS2, MMP9 and IL6 weFull Paper
2019Acosta, SA;Lee, JY;Nguyen, H;Kaneko, Y;Borlongan, CV;Endothelial Progenitor Cells Modulate Inflammation-Associated Stroke VasculomeStem cell reviews and reports30739275linded to the treatment condition. Cell Culture Procedures EPC Differentiation from Human Bone Marrow Mesenchymal Stem Cells Frozen human bone marrow CD34+ mesenchymal stem cells were purchased from ALLCELLS (Catalog# ABM017F; Alameda, CA). After thFull Paper
2019Meehan, S;Kolyagin, GA;Parks, D;Youngyunpipatkul, J;Herzenberg, LA;Walther, G;Ghosn, EEB;Orlova, DY;Automated subset identification and characterization pipeline for multidimensional flow and mass cytometry data clustering and visualizationCommunications biology31240267Fresh bone marrow mononuclear cells from adult healthy donors were obtained commercially from AllCells, LLC (Quincy, MA, Cat # ABM024). Data was collected for about 0.5-1.0 × 10 SaveFull Paper
2019Guo, X;Yuan, Z;Xu, Y;Zhao, X;Fang, Z;Yuan, WE;A Low-Molecular-Weight Polyethylenimine/pDNA-VEGF Polyplex System Constructed in a One-Pot Manner for Hindlimb Ischemia TherapyPharmaceutics30965617Aldrich. VEGF-A (1239 bp) pDNA encoding GFP was constructed by Bioroot Biology (Shanghai, China). 2.2. Cell Culture Human umbilical vein endothelial cells (HUVECs, primary cells) were purchased from ALLCELLS (Shanghai, China) and cultured in RPMI-16Full Paper
2019Zhang, M;Xu, Y;Jiang, L;Irisin attenuates oxidized low-density lipoprotein impaired angiogenesis through AKT/mTOR/S6K1/Nrf2 pathwayJournal of cellular physiology30942905Commercial HUVECs were obtained from Allcells (Shanghai, China). Cells were seeded in DMEM/F12 medium with 20% FBS (Gibco), 60 μg/ml endothelial cell growth supplement (BD, San Diego, CA) and maintained at 37°C in a CO2 incubator. The HMEC-1 cells weFull Paper
2019Wang, J;Fei, K;Jing, H;Wu, Z;Wu, W;Zhou, S;Ni, H;Chen, B;Xiong, Y;Liu, Y;Peng, B;Yu, D;Jiang, H;Liu, J;Durable blockade of PD-1 signaling links preclinical efficacy of sintilimab to its clinical benefitmAbs31402780× MSD read buffer with surfactant (R92TC-1, MSD) was added into each well. The electrochemiluminescent signal was then measured on the MSD SQ120 instrument. In vitro PD-1 receptor occupancy PBMCs (AllCells, Alameda, California, USA) were activated bFull Paper
2019Ovacik, AM;Li, J;Lemper, M;Danilenko, D;Stagg, N;Mathieu, M;Ellerman, D;Gupta, V;Kalia, N;Nguy, T;Plaks, V;David Johnson, C;Wang, W;Brumm, J;Fine, B;Junttila, T;Lin, K;Carter, PJ;Prabhu, S;Spiess, C;Kamath, AV;Single cell-produced and in vitro-assembled anti-FcRH5/CD3 T-cell dependent bispecific antibodies have similar in vitro and in vivo propertiesmAbs30550367100 (number of live target cells without TDB - number of live target cells with TDB)/number of live target cells without TDB. Human BM aspirates from healthy donors (n = 2, male) (procured from AllCells, ABM001-1) were diluted in phosphate-bufferedFull Paper
2019Wang, H;He, H;Yang, C;miR-342 suppresses the proliferation and invasion of acute myeloid leukemia by targeting Naa10pArtificial cells, nanomedicine, and biotechnology31496296The MNC normal control samples were isolated from normal BM cells purchased from AllCells, LLC (Emeryville, CA) by use of NycoPrep 1.077 A (Axis-Shield, Oslo, Norway) according SaveFull Paper
2019Chang, AY;Hsu, E;Patel, J;Li, Y;Zhang, M;Iguchi, H;Rogoff, HA;Evaluation of Tumor Cell-Tumor Microenvironment Component Interactions as Potential Predictors of Patient Response to NapabucasinMolecular cancer research : MCR31043490pSTAT3 is a potential biomarker for patient response to the anticancer drug napabucasin. Visual Overview: http://mcr.aacrjournals.org/content/molcanres/17/7/1429/F1.large.jpg . SaveFull Paper
2019Marklein, RA;Klinker, MW;Drake, KA;Polikowsky, HG;Lessey-Morillon, EC;Bauer, SR;Morphological profiling using machine learning reveals emergent subpopulations of interferon-γ-stimulated mesenchymal stromal cells that predict immunosuppressionCytotherapy30503100Human bone marrow-derived MSCs were obtained from 11 different donors purchased from Lonza and AllCells at passage two (see Supplementary Table 1 for donor specifications). A SaveFull Paper
2019Sei, J;Moses, B;Becker, A;Kim, M;Kaur, N;Vemuri, M;Civin, C;StemPro HSC expansion medium (Prototype) supports superior expansion of human hematopoietic stem-progenitor cellsCytotherapySingle donor, cord-blood derived, CD34+ cells obtained from AllCells were thawed and cultured in CTS StemPro HSC expansion media containing SCF, FLT3, TPO, IL3 and IL6 growth SaveFull Paper
2019Kou, X;Sun, Y;Li, S;Bian, W;Liu, Z;Zhang, D;Jiang, J;Pharmacology Study of the Multiple Angiogenesis Inhibitor RC28-E on Anti-Fibrosis in a Chemically Induced Lung Injury ModelBiomolecules31652997The human skin fibroblast cell line Detroit 551 was purchased from AllCells (Shanghai, China) and maintained in MEM medium (Gibco, Grand Island, NY, USA) supplemented with 10% SaveFull Paper
2019Zhang, Y;Yu, S;Tuazon, JP;Lee, JY;Corey, S;Kvederis, L;Kingsbury, C;Kaneko, Y;Borlongan, CV;Neuroprotective effects of human bone marrow mesenchymal stem cells against cerebral ischemia are mediated in part by an anti-apoptotic mechanismNeural regeneration research30632499Bcl-2 antibody was 58, 117, or 235 ng/mL. Both hMSC and Bcl-2 doses were based on pilot studies demonstrating their potencies. Cryopreserved human bone marrow CD34+ cells (hBM34+) were purchased from AllCells (Alameda, CA, USA). The exposure time ofFull Paper
2019Weisgrau, KL;Vosler, LJ;Pomplun, NL;Hayes, JM;Simmons, HA;Friedrichs, KR;Rakasz, EG;Neutrophil progenitor populations of rhesus macaquesJournal of leukocyte biology30395351We purchased de-identified, freshly obtained human bone marrow and matching blood samples from 18-65 years old healthy individuals from AllCells LLC (Boston, USA), or Lonza Inc. (Mapleton, IL, USA). The donors signed a procedure-specific consent formFull Paper
2019Cao, L;Weetall, M;Trotta, C;Cintron, K;Ma, J;Kim, MJ;Furia, B;Romfo, C;Graci, JD;Li, W;Du, J;Sheedy, J;Hedrick, J;Risher, N;Yeh, S;Qi, H;Arasu, T;Hwang, S;Lennox, W;Kong, R;Petruska, J;Moon, YC;Babiak, J;Davis, TW;Jacobson, A;Almstead, NG;Branstrom, A;Colacino, JM;Peltz, SW;Targeting of Hematologic Malignancies with PTC299, A Novel Potent Inhibitor of Dihydroorotate Dehydrogenase with Favorable Pharmaceutical PropertiesMolecular cancer therapeutics30352802AML cells, purchased from AllCells (January 2017), Inc and Champions Oncology, Inc (April 2017), were cultured in StemSpan medium (Stemcell Technologies, Vancouver). Cell lines in culture were routinely verified once per month to be mycoplasma free aFull Paper
2019Boothello, RS;Patel, NJ;Sharon, C;Abdelfadiel, EI;Morla, S;Brophy, DF;Lippman, HR;Desai, UR;Patel, BB;A Unique Nonsaccharide Mimetic of Heparin Hexasaccharide Inhibits Colon Cancer Stem Cells via p38 MAP Kinase ActivationMolecular cancer therapeutics30337351The cells were resuspended in 10 mL of Iscove's modified Dulbecco's medium (2% FBS media; Allcells) and mixed with Methyl cellulose (R&D systems), and 1.1 mL of the final cell SaveFull Paper
2019Suhito, IR;Kang, ES;Kim, DS;Baek, S;Park, SJ;Moon, SH;Luo, Z;Lee, D;Min, J;Kim, TH;High density gold nanostructure composites for precise electrochemical detection of human embryonic stem cells in cell mixtureColloids and surfaces. B, Biointerfaces31082776For the comparison, cryopreserved hCB-EPCs were purchased from AllCells, LLC (California, USA) [26,27]. The hCB-EPCs were thawed in a 37 °C warm bath for 2 min and washed twice with 100 μl DNase (Sigma-Aldrich) in 10 ml PBS. Cells were cultured on aFull Paper
2019Xu, P;Ganaie, SS;Wang, X;Wang, Z;Kleiboeker, S;Horton, NC;Heier, RF;Meyers, MJ;Tavis, JE;Qiu, J;Endonuclease Activity Inhibition of the NS1 Protein of Parvovirus B19 as a Novel Target for Antiviral Drug DevelopmentAntimicrobial agents and chemotherapy30530599Primary human CD34+ hematopoietic stem cells (HSCs) were isolated from the bone marrow of a healthy human donor. We purchased them from AllCells LLC (Alameda, CA), and B19V-containng plasma samples were obtained from ViraCor Eurofins Laboratories (LeFull Paper
2019Patel, PR;Senyuk, V;Rodriguez, NS;Oh, AL;Bonetti, E;Mahmud, D;Barosi, G;Mahmud, N;Rondelli, D;Synergistic Cytotoxic Effect of Busulfan and the PARP Inhibitor Veliparib in Myeloproliferative NeoplasmsBiology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation30615982Normal CD34 + cells used as healthy controls were obtained from apheresis collection or cord blood (AllCells, Alameda, CA). Mononuclear cells were subsequently obtained by SaveFull Paper
2019Campos, JM;Sousa, AC;Caseiro, AR;Pedrosa, SS;Pinto, PO;Branquinho, MV;Amorim, I;Santos, JD;Pereira, T;Mendonça, CM;Afonso, A;Atayde, LM;Maurício, AC;Dental pulp stem cells and Bonelike® for bone regeneration in ovine modelRegenerative biomaterials30740242onmental SEM with X-ray microanalysis and electron backscattered diffraction analysis: Quanta 400 FEG ESEM/EDAX Genesis X4M . hMSC preparation Cell culture and maintenance hDPSCs were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01)Full Paper
2019Dai, L;Hu, W;Yang, Z;Chen, D;He, B;Chen, Y;Zhou, L;Xie, H;Wu, J;Zheng, S;Upregulated expression of HOXB7 in intrahepatic cholangiocarcinoma is associated with tumor cell metastasis and poor prognosisLaboratory investigation; a journal of technical methods and pathology30664713Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Shanghai, China; allcells.biomart.cn) and maintained in DMEM supplemented with 10% fetal bovine SaveFull Paper
2019Sachdeva, M;Duchateau, P;Depil, S;Poirot, L;Valton, J;Granulocyte-macrophage colony-stimulating factor inactivation in CAR T-cells prevents monocyte-dependent release of key cytokine release syndrome mediatorsThe Journal of biological chemistry30804212Cryopreserved human PBMCs were acquired from ALLCELLS (catalog no. PB006F), and human monocytes were acquired from STEMCELL Technologies (catalog no. 70035.1). Both PBMCs and monocytes were cultured in X-vivo-15 media (Lonza, catalog no. BE04-418Q),Full Paper
2019Yamamoto, R;Xu, Y;Ikeda, S;Sumida, K;Tanaka, H;Hozumi, K;Takaori-Kondo, A;Minato, N;Thymic Development of a Unique Bone Marrow-Resident Innate-like T Cell Subset with a Potent Innate Immune FunctionJournal of immunology (Baltimore, Md. : 1950)31085589Human BM mononuclear cells from healthy donors were purchased from AllCells (Alameda, CA). T cells, B cells, myeloid cells, and dendritic cells were isolated from BM cells by SaveFull Paper
2019Nikitin, PA;Rose, EL;Byun, TS;Parry, GC;Panicker, S;C1s Inhibition by BIVV009 (Sutimlimab) Prevents Complement-Enhanced Activation of Autoimmune Human B Cells In VitroJournal of immunology (Baltimore, Md. : 1950)30635392Primary PBMC from de-identified patients with rheumatoid arthritis were purchased from AllCells. No therapeutic modalities were used as exclusion criteria for samples from patients SaveFull Paper
2019Wu, J;Chen, L;Wang, R;Song, Z;Shen, Z;Zhao, Y;Huang, S;Lin, Z;Exosomes Secreted by Stem Cells from Human Exfoliated Deciduous Teeth Promote Alveolar Bone Defect Repair through the Regulation of Angiogenesis and OsteogenesisACS biomaterials science & engineering33405738(FBS; Gibco), 100 U/mL penicillin G, as well as 100 mg/mL streptomycin. HUVECs were bought from ALLCELLS (Shanghai, China) and expanded in endothelial cell growth medium (EGM). BMSCs were harvested from the femoral bones of SD rats. All experimentalFull Paper
2019Huang, YJ;Lee, CY;Cao, J;Lee, HS;Chang, CH;Chen, PD;Wu, YM;Therapeutic Potential of Plasma Proteins Derived from Umbilical Cord Blood for Acute Liver FailureMolecular pharmaceutics30698974There are very limited clinically viable treatment options for acute liver failure, a life-threatening condition that rapidly progresses to loss of liver function. In this study, we aim to evaluate SaveFull Paper
2019Seo, A;Gulsuner, S;Pierce, S;Ben-Harosh, M;Shalev, H;Walsh, T;Krasnov, T;Dgany, O;Doulatov, S;Tamary, H;Shimamura, A;King, MC;Inherited thrombocytopenia associated with mutation of UDP-galactose-4-epimerase (GALE)Human molecular genetics30247636CD34+ cells from human cord blood were purchased from AllCells. Thawed cells were expanded for 4 h at 37C in tissue culture incubator with X-VIVO10 media (Lonza) supplemented SaveFull Paper
2019Moore, N;Chevillet, JR;Healey, LJ;McBrine, C;Doty, D;Santos, J;Teece, B;Truslow, J;Mott, V;Hsi, P;Tandon, V;Borenstein, JT;Balestrini, J;Kotz, K;A Microfluidic Device to Enhance Viral Transduction Efficiency During Manufacture of Engineered Cellular TherapiesScientific reports31641163Both Research Blood Components and AllCells obtain IRB approved consent from all donors giving permission to collect their blood or blood product and use or sell it for research SaveFull Paper
2019Fischer, CL;Bates, AM;Lanzel, EA;Guthmiller, JM;Johnson, GK;Singh, NK;Kumar, A;Vidva, R;Abbasi, T;Vali, S;Xie, XJ;Zeng, E;Brogden, KA;Computational Models Accurately Predict Multi-Cell Biomarker Profiles in Inflammation and CancerScientific reports31350446experiments, GE KER were suspended at 1 × 105 viable cells/ml in Lymphocyte Growth Medium-3 (LGM-3, Lonza Walkersville, Inc., Walkersville, MD). Human monocyte-derived immature myeloid DC (DC, AllCells, Alameda, CA) were positively identified by floFull Paper
2019Laner-Plamberger, S;Oeller, M;Poupardin, R;Krisch, L;Hochmann, S;Kalathur, R;Pachler, K;Kreutzer, C;Erdmann, G;Rohde, E;Strunk, D;Schallmoser, K;Heparin Differentially Impacts Gene Expression of Stromal Cells from Various TissuesScientific reports31076619ed by the ethical committee of federal state of Salzburg. All donors of BM, WAT and UC signed an informed consent concerning the research use of materials. Additionally, BM samples were obtained from AllCells, (Alameda, CA, USA, www.allcells.com/statFull Paper
2019Yea, K;Jones, TM;Jung, D;Shin, S;Arlian, BM;Han, KH;Zha, Z;Kim, MS;Oh, YS;Zhang, H;Lerner, RA;An agonist antibody prefers relapsed AML for induction of cells that kill each otherScientific reports30837591population studied here is much larger than that simply defined by CD34+ and CD38+ cells. Methods Culture of AML cells Human AML cells were isolated from patients and frozen at −80 °C (ABM010, AllCells). The informed consent was obtained from all paFull Paper
2019Das, P;Panda, SK;Agarwal, B;Behera, S;Ali, SM;Pulse, ME;Solomkin, JS;Opal, SM;Bhandari, V;Acharya, S;Novel Chitohexaose Analog Protects Young and Aged mice from CLP Induced Polymicrobial SepsisScientific reports30814582mg/kg to young adult mice and 25 mg/kg to aged mice in a 0.2 mL volume near the dorsal scapular region. Cell lines Human peripheral blood mononuclear cells (HPBMCs) were purchased from LeukoPaK (AllCells); 0.5 × 106 cells/well were seeded in 96-wellFull Paper
2019Kizilyer, A;Singh, MV;Singh, VB;Suwunnakorn, S;Palis, J;Maggirwar, SB;Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces ThrombopoiesisScientific reports30808933CD34+ cells derived from BM or umbilical cord blood were purchased from Allcells (Emeryville, CA, USA), and differentiated as previously described 48 . For inducing MK progenitor ( SaveFull Paper
2019Zarriello, S;Neal, EG;Kaneko, Y;Borlongan, CV;T-Regulatory Cells Confer Increased Myelination and Stem Cell Activity after Stroke-Induced White Matter InjuryJournal of clinical medicine31010132Purified BMSCs were obtained and stored in aliquots in dry ice (AllCells, Almeda, CA, USA). After the cells reached confluence, they were subjected to an oxygen-glucose deprivation SaveFull Paper
2019Hu, B;Zou, Y;Zhang, L;Tang, J;Niedermann, G;Firat, E;Huang, X;Zhu, X;Nucleofection with Plasmid DNA for CRISPR/Cas9-Mediated Inactivation of Programmed Cell Death Protein 1 in CD133-Specific CAR T CellsHuman gene therapy29706119Frozen peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from ALLCELLS (PB005F). The tumor cell lines used in this study were generated based on a SaveFull Paper
2019Bednar, KJ;Nycholat, CM;Rao, TS;Paulson, JC;Fung-Leung, WP;Macauley, MS;Exploiting CD22 To Selectively Tolerize Autoantibody Producing B-Cells in Rheumatoid ArthritisACS chemical biology30835424Frozen peripheral blood mononuclear cells (PBMCs) were obtained through AllCells (Alameda, CA) or BioreclamationIVT (Chestertown, MD) as per institutional protocols for human sample acquisition. Cells were thawed in accordance with the manufacture’sFull Paper
2019Schott, JW;León-Rico, D;Ferreira, CB;Buckland, KF;Santilli, G;Armant, MA;Schambach, A;Cavazza, A;Thrasher, AJ;Enhancing Lentiviral and Alpharetroviral Transduction of Human Hematopoietic Stem Cells for Clinical ApplicationMolecular therapy. Methods & clinical development31338385lus HSC Brew GMP supplement (Miltenyi Biotec, Surrey, UK) additionally supplemented with 2% human albumin. For the GMP experiment, HD CD34+ HSPCs were isolated from a fresh mobilized leukapheresis (AllCells, Alameda, CA, USA) following standard immuFull Paper
2019Ellison, SM;Liao, A;Wood, S;Taylor, J;Youshani, AS;Rowlston, S;Parker, H;Armant, M;Biffi, A;Chan, L;Farzaneh, F;Wynn, R;Jones, SA;Heal, P;Gaspar, HB;Bigger, BW;Pre-clinical Safety and Efficacy of Lentiviral Vector-Mediated Ex Vivo Stem Cell Gene Therapy for the Treatment of Mucopolysaccharidosis IIIAMolecular therapy. Methods & clinical development31044143CD34 + cells were positively selected from two pooled PB mononuclear cell (PBMC) apheresis units (Allcells, USA) using the Miltenyi CliniMACSPlus instrument and reagents Save;00 for a further 24 hrs prior to seeding in CFU assay. Large-Scale TransFull Paper
2019Liu, W;Zhang, C;Wang, L;Huang, X;Zhang, J;He, Y;Chen, L;Li, J;Successful reversal of ovarian hyperstimulation syndrome in a mouse model by rapamycin, an mTOR pathway inhibitorMolecular human reproduction31329230HUVEC were purchased from ALLCELLS and seeded on micropore inserts (pore size 0.4 μm, Corning) in a density of 3 × 10 5 cells per insert and cultured overnight with or without 40 SaveFull Paper
2019Cheng, Y;Dai, X;Yang, T;Zhang, N;Liu, Z;Jiang, Y;Low Long Noncoding RNA Growth Arrest-Specific Transcript 5 Expression in the Exosomes of Lung Cancer Cells Promotes Tumor AngiogenesisJournal of oncology31186629ells were purchased from the Chinese Academy of Sciences Type Culture Collections Cell Bank (Shanghai, China). The primary human umbilical vein endothelial cells (HUVECs) were purchased from Shanghai AllCells Biotechnology Company (Shanghai, China).Full Paper
2019Kim, CH;Lim, CY;Lee, JH;Kim, KC;Ahn, JY;Lee, EJ;Human Embryonic Stem Cells-Derived Mesenchymal Stem Cells Reduce the Symptom of Psoriasis in Imiquimod-Induced Skin ModelTissue engineering and regenerative medicine30815354The PBMCs were purchased from AllCells, LLC. The hE-MSCs were inactivated by treatment with 10 μg/mL mitomycin-C (Sigma-Aldrich, St. Louis, MO, USA) for 1 h at 37 C. SaveFull Paper
2019Geldenhuys, WJ;Nair, RR;Piktel, D;Martin, KH;Gibson, LF;The MitoNEET Ligand NL-1 Mediates Antileukemic Activity in Drug-Resistant B-Cell Acute Lymphoblastic LeukemiaThe Journal of pharmacology and experimental therapeutics31010844Primary CD3 + T cells, CD19 + B cells, peripheral blood mononuclear cells, and bone marrow mononuclear cells were purchased from AllCells (Allcells.com) and maintained in SaveFull Paper
2019Xu, L;Zhao, G;Zhu, H;Wang, S;Sun, A;Zou, Y;Ge, J;Peroxisome Proliferator-Activated Receptor-γ Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5pPPAR research31354796Quantitative analysis of stained lipid area was performed by a blinded observer using Leica QWin V3 software. 2.3. In Vitro HUVECs Culture and Cell Immunostaining Primary HUVECs were purchased from AllCells (Shanghai, China) and cultured in low-glucFull Paper
2019Zhao, S;Molina, A;Yu, A;Hanson, J;Cheung, H;Li, X;Natkunam, Y;High frequency of CD74 expression in lymphomas: implications for targeted therapy using a novel anti-CD74-drug conjugateThe journal of pathology. Clinical research30191677egression analysis with one site‐specific binding equation on GraphPad Prism (La Jolla, CA, USA). Whole bone marrow (BM) aspirates (3 ml each) from five healthy human donors was obtained through AllCells, Inc. (Alameda, CA, USA). FACS binding on BMFull Paper
2019Xia, F;Yang, C;Wang, Y;Youcef-Toumi, K;Reuter, C;Ivanov, T;Holz, M;Rangelow, IW;Lights Out! Nano-Scale Topography Imaging of Sample Surface in Opaque Liquid Environments with Coated Active Cantilever ProbesNanomaterials (Basel, Switzerland)31337145Immersion test for whole blood sample with Ethylenediaminetetraacetic acid (EDTA) anti-coagulation treatment from AllCells LLC was also conducted. The coated probe worked well at SaveFull Paper
2019Yu, Y;Yoo, SM;Park, HH;Baek, SY;Kim, YJ;Lee, S;Kim, YL;Seo, KW;Kang, KS;Preconditioning with interleukin-1 beta and interferon-gamma enhances the efficacy of human umbilical cord blood-derived mesenchymal stem cells-based therapy via enhancing prostaglandin E2 secretion and indoleamine 2,3-dioxygenase activity in dextran sulfate sodium-induced colitisJournal of tissue engineering and regenerative medicine312930882.7 Isolation of CD4+ T cells with magnetic-activated cell sorting (MACS) CD4+ T cells were purified from PBMCs (ALLCells, Alameda, CA, USA) using MACS (Miltenyi Biotec, Bergisch Gladbach, Germany) as previously discribed (Hong et al., 2017)Full Paper
2019Yin, Y;Guo, J;Teng, F;Yu, L;Jiang, Y;Xie, K;Jiang, M;Fang, J;Preparation of a Novel One-Armed Anti-c-Met Antibody with Antitumor Activity Against Hepatocellular CarcinomaDrug design, development and therapy31849449bovine serum (FBS, qualified, Australia#10099-141; Gibco Co., Ltd) in a humidified incubator in the presence of 5% CO2 at 37°C. Human umbilical vein endothelial cells (HUVECs) were obtained from the Allcells Co., Ltd. (Shanghai, China). The cells werFull Paper
2019Kono, M;Matsuhiroya, S;Nakazawa, F;Kaido, M;Wada, A;Tomiyama, Y;Morphological and optical properties of human immature platelet-enriched population produced in immunodeficient micePlatelets30059642The animals were fed a pellet chow and sterilized tap water ad libitum. Human CD34-positive cells (1 × 105) from cord blood (AllCells, CA, USA) were transplanted through the tail vein after irradiation with 2.5 Gy. Eltrombopag at 25 mg/kg body weightFull Paper
2019Gangolli, RA;Devlin, SM;Gerstenhaber, JA;Lelkes, PI;Yang, M;A Bilayered Poly (Lactic-Co-Glycolic Acid) Scaffold Provides Differential Cues for the Differentiation of Dental Pulp Stem CellsTissue engineering. Part A29984629Human DPSCs were purchased from AllCells LLC (Emeryville, CA), and cultured in a basal medium comprised of 85% MEM Alpha (Gibco ThermoFisher Scientific, Waltham, MA) supplemented with 15% fetal bovine serum (Stem Cell qualified; ThermoFisher ScientifFull Paper
2019Huang, L;Tan, J;Li, W;Zhou, L;Liu, Z;Luo, B;Lu, L;Zhou, C;Functional polyhedral oligomeric silsesquioxane reinforced poly(lactic acid) nanocomposites for biomedical applicationsJournal of the mechanical behavior of biomedical materials30500698The sterilization step of samples was similar to above films. HUVECs (purchased from Allcells, shanghai, China) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin (P/S, Gibco)Full Paper
2019Cackowski, FC;Wang, Y;Decker, JT;Sifuentes, C;Weindorf, S;Jung, Y;Wang, Y;Decker, AM;Yumoto, K;Szerlip, N;Buttitta, L;Pienta, KJ;Morgan, TM;Taichman, RS;Detection and isolation of disseminated tumor cells in bone marrow of patients with clinically localized prostate cancerThe Prostate31449673Normal marrow samples were purchased from AllCells (Alameda, CA), drawn from the posterior superior iliac crest of paid donors, transferred to heparinized tubes and shipped SaveFull Paper
2019Zheng, Q;Zhang, X;Yang, H;Xie, J;Xie, Y;Chen, J;Yu, C;Zhong, C;Internal Ribosome Entry Site Dramatically Reduces Transgene Expression in Hematopoietic Cells in a Position-Dependent MannerViruses31597367anassas, VA, USA). Human embryonic kidney cell line 293T was purchased from the Institute of Biochemistry Cell Biology (Shanghai, China). The CD34+ hematopoietic stem cells (HSCs) were purchased from ALLCELLS (Alameda, CA, USA). The hepatocellular caFull Paper
2019Zhao, H;Chen, L;Zhong, G;Huang, Y;Zhang, X;Chu, C;Chen, L;Wang, M;Titanium Dioxide Nanoparticles Induce Mitochondrial Dynamic Imbalance and Damage in HT22 CellsJournal of NanomaterialsHT22 cells were from AllCells (Shanghai Biological Technology Co. Ltd., Shanghai, China). We carried out some experiments in the BE(2)C cell line, which is a human neuroblastoma cell line. The cells were planted in Dulbecco’s minimum essential mediumFull Paper
2019Liang, YR;Zhang, T;Jia, P;Xu, XL;Fang, Y;Ding, XQ;Interaction between bone marrow-derived dendritic cells and miR-21 of tubular renal epithelial cells under hypoxiaEuropean review for medical and pharmacological sciences30840288cluster of differentiation 86), MHCII (major histocompatibility complex class II molecules), and CCR7 (eBioscience, Frankfurt, Germany). Primary Murine Renal TEC Culture Primary renal TECs were bought from AllCells (Allcells, LLC, Berkeley, CA, USA)Full Paper
2019Youngblood, BA;Brock, EC;Leung, J;Falahati, R;Bryce, PJ;Bright, J;Williams, J;Shultz, LD;Greiner, DL;Brehm, MA;Bebbington, C;Tomasevic, N;AK002, a Humanized Sialic Acid-Binding Immunoglobulin-Like Lectin-8 Antibody that Induces Antibody-Dependent Cell-Mediated Cytotoxicity against Human Eosinophils and Inhibits Mast Cell-Mediated Anaphylaxis in MiceInternational archives of allergy and immunology31401630Purified human natural killer (NK) cells (AllCells) were washed with culture medium and added at 450,000 cells/well (effector: target cell ratio 9:1). Plates were incubated for 4 h at 37C SaveFull Paper
2019Gautam, A;Donohue, D;Hoke, A;Miller, SA;Srinivasan, S;Sowe, B;Detwiler, L;Lynch, J;Levangie, M;Hammamieh, R;Jett, M;Investigating gene expression profiles of whole blood and peripheral blood mononuclear cells using multiple collection and processing methodsPloS one31809517and the PAXgene blood RNA tubes were immediately frozen, one set of triplicate EDTA tubes was stored at 4C, and the remaining tubes received further processing at the AllCells, SaveFull Paper
2019Caseiro, AR;Santos Pedrosa, S;Ivanova, G;Vieira Branquinho, M;Almeida, A;Faria, F;Amorim, I;Pereira, T;Maurício, AC;Mesenchymal Stem/ Stromal Cells metabolomic and bioactive factors profiles: A comparative analysis on the umbilical cord and dental pulp derived Stem/ Stromal Cells secretomePloS one31774816ngiogenesis. 2. Materials and methods 2.1. Human Mesenchymal Stem/ Stromal Cells characterization and conditioning protocol Umbilical Cord -MSCs [PromoCellTM (C-12971; Lot No. 1112304.2)] and DPSCs [AllCells, LLC (DP0037F, Lot No. DPSC090411-01)] weFull Paper
2019Manshouri, T;Verstovsek, S;Harris, DM;Veletic, I;Zhang, X;Post, SM;Bueso-Ramos, CE;Estrov, Z;Primary myelofibrosis marrow-derived CD14+/CD34- monocytes induce myelofibrosis-like phenotype in immunodeficient mice and give rise to megakaryocytesPloS one31569199nstitutional Review Board (MDACC IRB1) approved written informed consent had been obtained. The patients’ clinical data are provided in S1 Table. As controls, normal BM aspirates were obtained from AllCells (Alameda, CA). Low-density cells were fractFull Paper
2019Singer, JW;Al-Fayoumi, S;Taylor, J;Velichko, S;O'Mahony, A;Comparative phenotypic profiling of the JAK2 inhibitors ruxolitinib, fedratinib, momelotinib, and pacritinib reveals distinct mechanistic signaturesPloS one31560729eline Cell Technologies and Cell Applications. Peripheral blood mononuclear cells (PBMCs) and positively selected primary normal human CD19+ B cells, CD4+ T cells, and CD14+ monocytes were purchased (AllCells, Emeryville, CA) or were isolated in-housFull Paper
2019Gunawardena, TNA;Masoudian, Z;Rahman, MT;Ramasamy, TS;Ramanathan, A;Abu Kasim, NH;Dental derived stem cell conditioned media for hair growth stimulationPloS one31042749ulp stem cells obtained from human deciduous teeth (SHED) in comparison to CM produced using HFSCs. Methodology Cell culturing of SHED and HFSCs SHED and HFSCs Cells at Passage 1 were purchased from AllCells, USA and ICELLTIS, Labege, France respectFull Paper
2019Mamontov, P;Eberwine, RA;Perrigoue, J;Das, A;Friedman, JR;Mora, JR;A negative role for the interleukin-2-inducible T-cell kinase (ITK) in human Foxp3+ TREG differentiationPloS one31022269or pharmacological targeting of specific Tec kinases in inflammatory and autoimmune disease. Materials and methods Cell culture Naïve CD4+ CD45RA+ CD25- peripheral blood T cells were purchased from AllCells (Alameda, CA). For Th0 non-polarized condiFull Paper
2019Rytlewski, J;Deng, S;Xie, T;Davis, C;Robins, H;Yusko, E;Bienkowska, J;Model to improve specificity for identification of clinically-relevant expanded T cells in peripheral bloodPloS one30870493expansion, have broad clinical value. Methods Training cohort Whole blood samples collected from 10 healthy donors at three time points with 2-week intervals between collections were purchased from AllCells, LLC (Emeryville, CA). Among the 10 donorsFull Paper
2019Yoshida, M;Horiguchi, H;Kikuchi, S;Iyama, S;Ikeda, H;Goto, A;Kawano, Y;Murase, K;Takada, K;Miyanishi, K;Kato, J;Kobune, M;miR-7977 inhibits the Hippo-YAP signaling pathway in bone marrow mesenchymal stromal cellsPloS one30835743r127; ab76252) and anti-NF2/Merlin Ab (ab88957) were purchased from Abcam (Tokyo, Japan). Human BM CD34+ cells and three different lot numbers of MSCs from healthy volunteers (HV) were purchased from AllCells, LLC (Toronto, Canada) (HV-derived MSCs #Full Paper
2019Chung, JE;Magis, W;Vu, J;Heo, SJ;Wartiovaara, K;Walters, MC;Kurita, R;Nakamura, Y;Boffelli, D;Martin, DIK;Corn, JE;DeWitt, MA;CRISPR-Cas9 interrogation of a putative fetal globin repressor in human erythroid cellsPloS one30645582based on mobility using a separate FASC hemoglobin standard (Trinity biotech). Electroporation of CD34+ HSPCs with Cas9 RNPs HSPC electroporation was as described [28]. CD34+ HSPCs (G-CSF mobilized, Allcells, Inc.) were thawed and cultured in SFEM (Full Paper
2019Hu, X;Shen, G;Hu, T;Guan, G;Wang, L;Use of an in vitro dynamic culture system to assess flow shear forces upon cell adhesion within different structuresJournal of Chemical Technology & BiotechnologyHuman umbilical vein endothelial cells (HUVEC001), provided by Allcells Company (Shanghai, China), were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, South American) and 1% penicillin-streptomycin (GibFull Paper
2019Satani, N;Giridhar, K;Cai, C;Wewior, N;Norris, DD;Olson, SD;Aronowski, J;Savitz, SI;Aspirin in stroke patients modifies the immunomodulatory interactions of marrow stromal cells and monocytesBrain research31220426MSCs from fresh human bone marrow aspirates (AllCells, Alameda, CA) were prepared from 3 separate healthy volunteers using density centrifugation and plastic adherence as previously described (Diaz et al., 2017, Kota et al., 2017, Parsha et al., 2017Full Paper
2019Mohd Nor, NH;Berahim, Z;Azlina, A;Kannan, TP;Identification of novel fibroblast-like cells from stem cells from human exfoliated deciduous teethClinical oral investigations30847574Objectives This study aimed to differentiate and characterize fibroblast-like cells from stem cells from human exfoliated deciduous teeth (SHED). Materials and methods The SaveFull Paper
2019McIntyre, PW;Wu, JL;Kolte, R;Zhang, R;Gregory, RL;Bruzzaniti, A;Yassen, GH;The antimicrobial properties, cytotoxicity, and differentiation potential of double antibiotic intracanal medicaments loaded into hydrogel systemClinical oral investigations29943369Human dental pulp stem cells (DPSC) (AllCells, Alameda, CA, USA) were cultured in 24-well plates (Cat: TP9024, Alkali Scientific Inc.) at concentrations of 3 × 104 cells/well in α-MEM (HyClone Laboratories Inc., South Logan, UT, USA) with 10% heat-inFull Paper
2019Zhong, X;Gao, W;Wu, R;Liu, H;Ge, J;Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanismMolecular medicine reports31638185HUVECs were obtained from AllCells, LLC and cultured in ECM (Scien Cell Research Laboratories, Inc.). The 8-10th generations of HUVECs were used in this study and they were harvested for mRNA or protein detection after treatment with exosomes for 6 oFull Paper
2019Chen, T;Li, T;Wang, J;p53 mediates PEDF‑induced autophagy in human umbilical vein endothelial cells through sestrin2 signalingMolecular medicine reports31173218ed to investigate the association between the PEDF-p53-sestrin pathway and autophagy in human umbilical vein endothelial cells (HUVECs). Materials and methods Cell culture HUVECs were purchased from AllCells (Alameda, CA, USA). Cells were cultured iFull Paper
2019Feitosa, SA;Palasuk, J;Geraldeli, S;Windsor, LJ;Bottino, MC;Physicochemical and biological properties of novel chlorhexidine-loaded nanotube-modified dentin adhesiveJournal of biomedical materials research. Part B, Applied biomaterials30199597The cytotoxicity of the CHX-loaded nanotube-modified adhesive and control groups (SBMP and HNT) were evaluated using human dental pulp stem cells (hDPSC, AllCells, Alameda, SaveFull Paper
2019Kim, MM;Schlussel, L;Zhao, L;Himburg, HA;Dickkopf-1 Treatment Stimulates Hematopoietic Regenerative Function in Infused Endothelial Progenitor CellsRadiation research31081743Human bone marrow CD34+ cells (AllCells®, Alameda, CA) were irradiated at 3 Gy and added in noncontact to the upper portion of the transwell at a dose of 10,000 cells/well. Cells were cultured for 7 days, viable cells were enumerated by trypan blue eFull Paper
2019Choi, HW;Chan, C;Shterev, ID;Lynch, HE;Robinette, TJ;Johnson-Weaver, BT;Shi, J;Sempowski, GD;Kim, SY;Dickson, JK;Gooden, DM;Abraham, SN;Staats, HF;Identification of Novel Mast Cell Activators Using Cell-Based High-Throughput ScreeningSLAS discovery : advancing life sciences R & D30917061Primary human mast cells were differentiated from human CD34+ hematopoietic stem cells.19 Human CD34+ hematopoietic stem cells were obtained from commercial sources (Allcells). Isolated CD34+ cells was suspended at 5 × 105 cells/mL in IMDM medium supFull Paper
2019Patel, A;Clementelli, CM;Jarocha, D;Mosoyan, G;Else, C;Kintali, M;Fong, H;Tong, J;Gordon, R;Gillespie, V;Keyzner, A;Poncz, M;Hoffman, R;Iancu-Rubin, C;Pre-clinical development of a cryopreservable megakaryocytic cell product capable of sustained platelet production in miceTransfusion31802511Division of Hematology and Medical Oncology, Tisch Cancer Institute and the Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York.++Division of Hematology and Medical Oncology, Tisch Cancer Institute and the Department oFull Paper
2019Md Hashim, S;Yusof, M;Zahari, W;Chandra, H;Ahmad Amin Noordin, K;Kannan, T;Sheikh Abdul Hamid, S;Mokhtar, K;Azlina, A;Human Amniotic Membrane as a Matrix for Endothelial Differentiation of VEGF-Treated Dental Stem CellsCellular and Molecular BioengineeringSHED (AllCells, USA) were cultured in the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, USA) supplemented with 15% of fetal bovine serum (FBS) (Gibco, USA) and 50 U/mL of penicillin-streptomycin (Gibco, USA). The cells were incubated inFull Paper
2019Juillerat, A;Tkach, D;Busser, BW;Temburni, S;Valton, J;Duclert, A;Poirot, L;Depil, S;Duchateau, P;Modulation of chimeric antigen receptor surface expression by a small molecule switchBMC biotechnology31269942witching-off CAR T-cells. Overall, this could mitigate some of the toxicities that could occur with early intense antitumor responses. Methods T-cell proliferation Cryopreserved human PBMCs/T-cells (ALLCELLS, cat # PB006F) were used in accordance wiFull Paper
2019Kim, OH;Yoon, OJ;Lee, HJ;Silk fibroin scaffolds potentiate immunomodulatory function of human mesenchymal stromal cellsBiochemical and biophysical research communications31506179Although mesenchymal stromal cells (MSCs) show great potential for use in regenerative medicine, their therapeutic efficacy remains limited because of their low adaptation efficiency SaveFull Paper
2019Luo, H;Hu, L;Ma, B;Zhao, M;Luo, M;Deng, Q;Deng, S;Ye, H;Lin, T;Chen, J;Wang, T;Zhu, J;Lu, H;Molecular dynamics based improvement of the solubilizing self-cleavable tag Zbasic-ΔI-CM application in the preparation of recombinant proteins in Escherichia coliBiochemical and biophysical research communications30967267HUVEC was obtained from ATCC (Cat# CRL-1730) and cultured in ECM medium supplemented with 5% fetal bovine serum and 1 × ECGS (Allcells; Shanghai, China). For proliferation detection, the cells were harvested and re-suspended using the assay medium (EFull Paper
2019Camarillo, JM;Swaminathan, S;Abshiru, NA;Sikora, JW;Thomas, PM;Kelleher, NL;Coupling Fluorescence-Activated Cell Sorting and Targeted Analysis of Histone Modification Profiles in Primary Human LeukocytesJournal of the American Society for Mass Spectrometry31286445De-identified donor peripheral blood (PB) specimen were obtained from AllCells (Alameda, CA), Innovative Research (Novi, MI), or as clinical discards from the Clinical Flow Cytometry Laboratory at Northwestern Memorial Hospital. All procedures perforFull Paper
2019Ishikawa, G;Fujiwara, N;Hirschfield, H;Varricchio, L;Hoshida, Y;Barosi, G;Rosti, V;Padilla, M;Mazzarini, M;Friedman, SL;Hoffman, R;Migliaccio, AR;Shared and Tissue-Specific Expression Signatures between Bone Marrow from Primary Myelofibrosis and Essential ThrombocythemiaExperimental hematology31678370Bone marrow from eight healthy individuals was purchased from AllCells Technology (Oakland, CA). mRNA in amounts sufficient for analyses was obtained from 6 PMF and all ET SaveFull Paper
2019Will, B;Kao, Y;Chen, J;Aivalioti, M;Tatiparthy, M;1029 - IRON HOMEOSTASIS-REGULATORY PATHWAYS IN HEMATOPOIETIC STEM CELLSExperimental HematologyHealthy cryopreserved human bone marrow cells and CD34+ enriched cells were purchased from AllCells (Alameda, CA) and ReachBio Research Labs (ReachBio LLC, WA). Fresh unprocessed bone marrow cells were purchased from Lonza (Lonza, MD), and the cellsFull Paper
2019Yang, H;Sun, J;Chen, H;Wang, F;Li, Y;Wang, H;Qu, T;Mesenchymal stem cells from bone marrow attenuated the chronic morphine-induced cAMP accumulation in vitroNeuroscience letters30605704Human bone marrow (n = 3) was purchased from AllCells Inc (Emeryville, CA). MSCs were isolated from the bone marrow according to previously described methods [16]. In brief, the bone marrow was diluted 1:1 with DMEM (low glucose, Invitrogen, CA, USA)Full Paper
2019Muruganandham, A;Dumont, C;Xing, K;Approaching stability challenges for flow cytometry in a regulated bioanalytical environmentBioanalysis31637937Mobilized human whole blood, with K 3 EDTA as anticoagulant, was purchased from ALLCELLS . Celerion stabilizer was added at 20% volume of blood within 24 h of collection and SaveFull Paper
2019Choi, SA;An, JH;Lee, SH;Lee, GH;Yang, HJ;Jeong, PS;Cha, JJ;Lee, S;Park, YH;Song, BS;Sim, BW;Kim, YH;Kim, JS;Jin, YB;Huh, JW;Lee, SR;Lee, JH;Kim, SU;Comparative Evaluation of Hormones and Hormone-Like Molecule in Lineage Specification of Human Induced Pluripotent Stem CellsInternational journal of stem cells31242719mising other lineage decisions. Materials and Methods Cell culture, hiPSC generation, and lineage specification To obtain hiPSCs, CD34+ cells were purified from primary human bone marrow (BM) cells (Allcells, Emeryville, CA, USA) via immunomagneticFull Paper
2019Kuramitsu, M;Okuma, K;Tezuka, K;Nakamura, H;Sagara, Y;Kurane, I;Hamaguchi, I;Development and evaluation of human T-cell leukemia virus-1 and -2 multiplex quantitative PCRMicrobiology and immunology31429972Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells Inc. (Alameda, CA), TL-Om1 cells have been reported previously 15 and the MoT cell line (CRL-8066) was purchased from ATCC (Manassas, VA). Plasmid DNA pX1MT-M,16 an HTLV-1 molecuFull Paper
2019Li, X;Li, X;Lin, J;Sun, X;Ding, Q;Exosomes Derived From Low-Intensity Pulsed Ultrasound-Treated Dendritic Cells Suppress Tumor Necrosis Factor-Induced Endothelial InflammationJournal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine30561085Human Umbilical Vein Endothelial Cell Culture Human umbilical vein endothelial cells were purchased from AllCells and cultured in endothelial cell medium (ScienCell, Shanghai, China) in a humidified atmosphere of 95% air and 5% CO2 at 37°CFull Paper
2019Choi, JH;Necsoiu, C;Wendorff, D;Jordan, B;Dixon, A;Roberts, TR;Beely, BM;Cancio, LC;Batchinsky, AI;Effects of adjunct treatments on end-organ damage and histological injury severity in acute respiratory distress syndrome and multiorgan failure caused by smoke inhalation injury and burnsBurns : journal of the International Society for Burn Injuries31378621The process of disqualifying AssureImmune and qualifying AllCells as a new source of bone marrow for the production of MSCs delayed our timeline. 2. Based on our published Phase SaveFull Paper
2019Ma, J;Lai, G;Lu, Z;Effect of 410 nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor (VEGF) in cultured human vascular endothelial cellsLasers in medical science30350123dilution with RPMI-1640 medium (ALLCELLS). Working drug solutions (0-8 μg/ml) were prepared by diluting the stock solution with RPMI-1640 medium (ALLCELLS. CA, USA). A light- SaveFull Paper
2019Miura, S;Kobune, M;Horiguchi, H;Kikuchi, S;Iyama, S;Murase, K;Goto, A;Ikeda, H;Takada, K;Miyanishi, K;Kato, J;EPO-R+ myelodysplastic cells with ring sideroblasts produce high erythroferrone levels to reduce hepcidin expression in hepatic cellsBlood cells, molecules & diseases31082798Total RNA derived from human BM CD34+, CB CD34+ and human mesenchymal stromal (MSCs) cells was purchased from AllCells, LLC (Toronto, Canada). Human myeloid leukemia SaveFull Paper
2019Li, Y;Zhao, R;Li, X;Wang, C;Bao, H;Wang, S;Fang, J;Huang, J;Wang, C;Blood-compatible Polyaniline Coated Electrospun Polyurethane Fiber Scaffolds for Enhanced Adhesion and Proliferation of Human Umbilical Vein Endothelial CellsFibers and Polymersprothrombin time agent were purchased from AllCells Biological Technology Co., Ltd. Phosphate buffered saline (PBS) was purchased from Beijing Dingguo Changsheng SaveFull Paper
2019Yamasaki, AE;King, NE;Matsui, H;Jepsen, K;Panopoulos, AD;Two iPSC lines generated from the bone marrow of a relapsed/refractory AML patient display normal karyotypes and myeloid differentiation potentialStem cell research317392017q and 13q, were obtained from AllCells, LLC. Immunohistochemistry analysis of the patient's a normal karyotype (both analyses provided by AllCells, LLC). Previously published iPSCs SaveFull Paper
2019CECHIM, G;CHIES, J;In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive reviewAnais da Academia Brasileira de CiênciasCommercial leukopaks generally contain 80-200 mL of processed material with approximately 7x109peripheral blood mononuclear cells (PBMC), (more information available on: www.allcells.com/products/whole-tissue/leuko-pak). The Table I shows the percentFull Paper
2019Coronado, RE;Somaraki-Cormier, M;Ong, JL;Halff, GA;Hepatocyte-like cells derived from human amniotic epithelial, bone marrow, and adipose stromal cells display enhanced functionality when cultured on decellularized liver substrateStem cell research31163390Bone Marrow Derived Mesenchymal Stromal/Stem Cells: Bone marrow derived mesenchymal stem cells (BM-MSCs) were isolated from whole bone marrow aspirates (AllCells Alameda SaveFull Paper
2019Abhyankar, VP;Bates, AM;Fischer, CL;Johnson, GK;Guthmiller, JM;Progulske-Fox, A;Brogden, KA;Dataset on the chemokine and cytokine responses of multi-cell cultures treated with Porphyromonas gingivalis hemagglutinin BData in brief30740479HagB (QCL-1000, Chromogenic Limulus Amebocyte Lysate Assay, Lonza, Inc. Walkersville, MD USA). 2.2 Dendritic cells, GE keratinocytes, and T cells Non-peripheral blood human myeloid dendritic cells (AllCells, LLC, Alameda, CA USA) were thawed and susFull Paper
2019Hii, SC;Luddin, N;Kannan, TP;Ab Rahman, I;Nik Abdul Ghani, NR;The Biological Evaluation of Conventional and Nano-Hydroxyapatite-Silica Glass Ionomer Cement on Dental Pulp Stem Cells: A Comparative StudyContemporary clinical dentistry32308298DPSCs purchased from AllCells, USA were used in this study. DPSCs were cultured in Alpha Minimum Essential Medium (α-MEM) (Gibco, Life Technologies, USA) supplemented with SaveFull Paper
2019Akers, S;Modeling central nervous system involvement in acute lymphoblastic leukemiaWest Virginia University LibrariesScientific Corporation, Westbury, NY) according to the manufacturer’s instructions. Positively selected peripheral blood CD19+ B-cells from a healthy donor were purchased from AllCells (Emeryville, CA). Human brain-derived microvascular endothelial cFull Paper
2019Barve, A;Characterization of a more clinically relevant human leukemia xenograft model to examine perturbation of MET/SAM metabolism as a novel therapeutic paradigm for MLL-R leukemia in vivo.University of LouisvilleAcute myeloid leukemia (AML), is a heterogeneous clonal disorder characterized by an accumulation of malignant myeloid progenitors in the bone marrow (BM), hindering normal SaveFull Paper
2019Liu, H;Kezios, S;DYNAMIC CHARACTERISTICS OF ETTINGSHAUSEN REFRIGERATORProceeding of International Heat Transfer Conference 4However this was not the case in allcells and in cells from plant 41, with 3 accessory chromo¬somes, sometimes none, one, two or all three accessorychromosomes divided precociously SaveFull Paper
2019Rodriguez, L;Mohammadipoor, A;Kamucheka, R;Asher, A;Alvarado, L;Antebi, B;Cancio, L;Preconditioning in an Acute Respiratory Distress Syndrome-Like Milieu Augments the Immunomodulatory and Anti-Inflammatory Function of MSCSA60. LUNG INJURY, SEPSIS, AND ARDSMethods: Human bone marrow (BM)-MSCs were isolated from fresh mononuclear cells purchased from AllCells (Alameda, CA). Human adipose-derived (AD)-MSCs were isolated from SaveFull Paper
2019De Maio, V;Brandic, I;Multi-Objective Mobile Edge Provisioning in Small Cell CloudsProceedings of the 2019 ACM/SPEC International Conference on Performance EngineeringAlso, we can observe significant losses when using the other two algorithms, ALLCELLS and RANDOM. In the first case this is because the algorithm overestimates the number of Edge SaveFull Paper
2019Lal, A;Chiang, Z;Yakovenko, N;Duarte, F;Israeli, J;Buenrostro, J;AtacWorks: A deep convolutional neural network toolkit for epigenomicsbioRxivCryopreserved human bone marrow mononuclear cells were purchased from Allcells (catalog number BM, CR, MNC, 10M). Cells were quickly thawed in a 37°C water bath, rinsed with culture medium (RPMI 1640 medium supplemented with 15% FBS) andFull Paper
2019Liu, J;Kozhaya, L;Torres, V;Unutmaz, D;Lu, M;Structure-Based Discovery of a Novel Small-Molecule Inhibitor of Methicillin-Resistant S. aureusbioRxivBlood samples were obtained from healthy, consenting donors as Buffy coats (New York Blood Center) and leukopaks (AllCells, Alameda, CA). PBMCs were isolated from peripheral blood by density gradient centrifugation using Ficoll-PaqueFull Paper
2019Wang, X;Derakhshandeh, R;Rodriguez, H;Han, D;Kostyushev, D;Henry, T;Traverse, J;Moyé, L;Simari, R;Taylor, D;Springer, M;Impaired Therapeutic Efficacy of Bone Marrow Cells from Post-Myocardial Infarction Patients in the TIME and LateTIME Clinical TrialsbioRxivHealthy de-identified donor BM MNCs were purchased from AllCells (Alameda, CA). These MNCs were manually isolated at AllCells using Ficoll conditions like that in the Sepax SaveFull Paper
2019Granja, J;Klemm, S;McGinnis, L;Kathiria, A;Mezger, A;Parks, B;Gars, E;Liedtke, M;Zheng, G;Chang, H;Majeti, R;Greenleaf, W;A single cell framework for multi-omic analysis of disease identifies malignant regulatory signatures in mixed phenotype acute leukemiabioRxivPBMCs, BMMCs, and CD34+ bone marrow cells were obtained from healthy donors (AllCells). Description of Leukemic Patients/Donors Patient samples were collected with informed consent prospectively under a protocol approved by the Institutional ReviewFull Paper
2019Ding, J;Adiconis, X;Simmons, S;Kowalczyk, M;Hession, C;Marjanovic, N;Hughes, T;Wadsworth, M;Burks, T;Nguyen, L;Kwon, J;Barak, B;Ge, W;Kedaigle, A;Carroll, S;Li, S;Hacohen, N;Rozenblatt-Rosen, O;Shalek, A;Villani, A;Regev, A;Levin, J;Systematic comparative analysis of single cell RNA-sequencing methodsbioRxivWe acquired commercially-available PBMCs (AllCells; Lot #PB006F-A5865) as frozen aliquots with 25 million cells for the PBMC1 experiment or isolated them from fresh blood (AllCells SaveFull Paper
2019Fan, L;Zhao, M;Gold nanorod based delivery system could bring about superior therapy effect of Ramucirumab through direct cytotoxicity to cancer cell mediated by differential regulation of phagocytosis in gastric cancer cellbioRxivHUVEC cell was purchased from Allcells (Endothelial Cell Medium, H-004B, Allcells, supplemented with 5%fetal bovine serum), GC cells SNU-5 (1640 Cell Medium, Gibco, supplemented with 10% fetal bovine serum) cell was purchased from ATCC and MKNFull Paper
2019King, GR;Comparative systemic analysis of human immunoglobulin repertoiresThesisAll peripheral blood and bone marrow aspirate samples were collected by AllCells and shipped overnight at ambient temperature; processing was started immediately upon receipt of the samples in a sterilized biological safety hood. Sterile 1x PBS pH 7.Full Paper
2019Sakhnevych, S;Tim-3-galectin-9 immunosuppressive pathway in human acute myeloid leukaemia and solid tumour cells and biochemical functions of its crucial componentsThesisPrimary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordance with the manufacturer's instructions following ethical approval (REC reference: 16-SS-033). Other pFull Paper
2019Lee, MJ;The Development of Novel Proteasome Inhibitors for the Treatment of Multiple Myeloma and Alzheimer's DiseaseThesisOver a decade, proteasome inhibitors (PIs), bortezomib, carfilzomib (Cfz) and ixazomib, have contributed to a significant improvement in the overall survival for multiple myeloma (MM) SaveFull Paper
2019Kuhlman, BM;MICROGRAVITY NEGATIVELY AFFECTS DNA DAMAGE REPAIR AND THE ANTI-LEUKEMIC FUNCTION OF HUMAN NATURAL KILLER CELLSThesisHealthy human bone marrow (BM) mononuclear cells (BMMNC) were purchased from AllCells, LLC (Alameda, CA, USA). CD34+ HSC were isolated from BMMNC using antihuman CD34 MicroBeads and the MiniMACS magnetic-based positive-selection system (Miltenyi BiotFull Paper
2019龚子祯, ;蔡海波, ;谭文松, ;黄原胶支持 CIK 细胞高密度扩增高校化学工程学报RPMI 1640购于美国Gibco公司; 血清培养基(自配);PBMC购于上海Allcells公司;CD3单克隆 抗体购于美国eBioscience公司;植物血凝 PHA购于美国Sigma-Aldrich公司;干扰 (IFN-γ),白介 SaveFull Paper
2019Cao, YP;Pan, M;Song, YL;Zhang, HL;MiR-302 a/b/c suppresses tumor angiogenesis in hepatocellular carcinoma by targeting MACC1European Review for Medical and Pharmacological SciencesThe umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Huh7 and PLC/PRF/5 cells were incubated with Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serFull Paper
2019王一唯, ;黄婷, ;孙笑, ;王玉东, ;RANKL 募集调节性 T 细胞促进子宫内膜癌侵袭的研究中国免疫学杂志科院细胞库),外周单个 细胞(美国ALLCELLS 公 司),RANK 慢病毒(上海和元生物科技公司),重组 人TGF鄄茁,重组人RANKL(美国Peprotech 公司),人 RANKL ELISA 试剂盒(武汉博士德生物 SaveFull Paper
2019Alvarado, L;Evaluating the Therapeutic Effects of Mesenchymal Stem Cells in an in Vitro Co-Culture System of a Damaged Alveolar BarrierThesisHuman BM-MSCs were isolated from commercially available MNCs (AllCells LLC; Emeryville, CA). Bone marrow specimen were obtained from human donors 20-30 years of age. MNCs were cultured on standard culture flasks at a density of 3 x 105 cells/cm2 inFull Paper
2019Romero-Masters, J;Studying Epstein-Barr virus-induced lymphomagenesis in a cord-blood humanized mouse modelThesisCreation of cord blood-humanized NOD/LtSz-scid/IL2Rγnull mice: Immunodeficient NSG (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson labs (catalogue number: 005557). Commercially available CD34-depleted human cord blood mononuclear cells (AlFull Paper
2019Mumbach, MR;Technologies for Protein and RNA-Focused Chromosome ConformationThesisNormal donor human peripheral blood cells were obtained fresh from AllCells. CD4+ T cells were enriched from peripheral blood using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). For CD4+ T helper cell subtypes, Naïve T cFull Paper
2019Walker, KL;Non-chemotherapeutic Methods of Treating T Cell Acute Lymphoblastic LeukemiaThesisChapter 4.2: EOpansion of NK cell products Fresh leukopaks were commercially o9tained (AllCells, Alameda, CA) from paid human apheresis donors, stored at 1Г10o C, and SaveFull Paper
2019Srivastava, S;Salter, AI;Liggitt, D;Yechan-Gunja, S;Sarvothama, M;Cooper, K;Smythe, KS;Dudakov, JA;Pierce, RH;Rader, C;Riddell, SR;Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor TargetingCancer cell30889382CFU Assays Femurs and tibia from control and ROR1 CAR-T cell-treated mice were harvested 9 days post-T cell transfer and submitted to ReachBio Inc. for CFU-GM, BFU-E, CFU-Mk, Pre-B CFC, and CFU-F assaysFull Paper
2019Humbert, O;Radtke, S;Samuelson, C;Carrillo, RR;Perez, AM;Reddy, SS;Lux, C;Pattabhi, S;Schefter, LE;Negre, O;Lee, CM;Bao, G;Adair, JE;Peterson, CW;Rawlings, DJ;Scharenberg, AM;Kiem, HP;Therapeutically relevant engraftment of a CRISPR-Cas9-edited HSC-enriched population with HbF reactivation in nonhuman primatesScience translational medicine31366580For CFC assays, 1000-1200 sorted cells were seeded into 3.5 ml ColonyGEL 1402 (ReachBio). Hematopoietic colonies were scored after 12-14 days. Arising colonies were identified as colony-forming unit- (CFU-) granulocyte (CFU-G), macrophageFull Paper
2019Crawford, A;Haber, L;Kelly, MP;Vazzana, K;Canova, L;Ram, P;Pawashe, A;Finney, J;Jalal, S;Chiu, D;Colleton, CA;Garnova, E;Makonnen, S;Hickey, C;Krueger, P;DelFino, F;Potocky, T;Kuhnert, J;Godin, S;Retter, MW;Duramad, P;MacDonald, D;Olson, WC;Fairhurst, J;Huang, T;Martin, J;Lin, JC;Smith, E;Thurston, G;Kirshner, JR;A Mucin 16 bispecific T cell-engaging antibody for the treatment of ovarian cancerScience translational medicine31217340Regeneron Pharmaceuticals Institutional Animal Care and Use Committee. NSG (20 to 24 weeks old) mice (the Jackson Laboratory) were injected with 5 × 106 human PBMCs (ReachBio) before 2 × 106 ascites cells from the OVCAR-3/Luc cell line,Full Paper
2019Li, C;Mishra, AS;Gil, S;Wang, M;Georgakopoulou, A;Papayannopoulou, T;Hawkins, RD;Lieber, A;Targeted Integration and High-Level Transgene Expression in AAVS1 Transgenic Mice after In Vivo HSC Transduction with HDAd5/35++ VectorsMolecular therapy : the journal of the American Society of Gene Therapy314940532,500 Lin− cells were plated in triplicates in ColonyGEL 1202 mouse complete medium (ReachBio, Seattle, WA) and incubated for 12 days at 37°C in 5% CO2 and maximum humidity. Colonies were enumerated using a Leica MS 5 dissection microscope (Leica MicFull Paper
2019Kuhlmann, AS;Haworth, KG;Barber-Axthelm, IM;Ironside, C;Giese, MA;Peterson, CW;Kiem, HP;Long-Term Persistence of Anti-HIV Broadly Neutralizing Antibody-Secreting Hematopoietic Cells in Humanized MiceMolecular therapy : the journal of the American Society of Gene Therapy30391142erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor (GM-CSF). At necropsy, cells isolated from bone marrow (BM) were seeded in triplicate into ColonyGEL (ReachBio, Seattle WA). HemFull Paper
2019Humbert, O;Laszlo, GS;Sichel, S;Ironside, C;Haworth, KG;Bates, OM;Beddoe, ME;Carrillo, RR;Kiem, HP;Walter, RB;Engineering resistance to CD33-targeted immunotherapy in normal hematopoiesis by CRISPR/Cas9-deletion of CD33 exon 2Leukemia30291334system (Harvard Apparatus, Cambridge, MA) [12]. For evaluation of colony-forming units (CFUs), 1500 CD34+ cells were seeded in 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA) and scored after 12-14 days. CFU DNA was extracted in QuickExtract (EpicentreFull Paper
2019Ly, M;Rentas, S;Vujovic, A;Wong, N;Moreira, S;Xu, J;Holzapfel, N;Bhatia, S;Tran, D;Minden, MD;Draper, JS;Hope, KJ;Diminished AHR Signaling Drives Human Acute Myeloid Leukemia Stem Cell MaintenanceCancer research31519687Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio), supplemented with DMSO or 750 SaveFull Paper
2019Smitheman, KN;Severson, TM;Rajapurkar, SR;McCabe, MT;Karpinich, N;Foley, J;Pappalardi, MB;Hughes, A;Halsey, W;Thomas, E;Traini, C;Federowicz, KE;Laraio, J;Mobegi, F;Ferron-Brady, G;Prinjha, RK;Carpenter, CL;Kruger, RG;Wessels, L;Mohammad, HP;Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypesHaematologica30514804uated for each assay. Primary acute myeloid leukemia blast colony Clonogenic progenitors from primary AML patients’ samples were assessed in a semi-solid methylcellulose-based medium (ColonyGel , ReachBio) containing recombinant human (rh) SCF (50 ngFull Paper
2019Fuller, NO;Pirone, A;Lynch, BA;Hewitt, MC;Quinton, MS;McKee, TD;Ivarsson, M;CoREST Complex-Selective Histone Deacetylase Inhibitors Show Prosynaptic Effects and an Improved Safety Profile To Enable Treatment of SynaptopathiesACS chemical neuroscience30496686Normal human bone marrow light density cells derived from normal bone marrow (NorCal Biologics, California) and qualified at ReachBio were stored in the gaseous phase of liquid Save;minimum peptide length of at least 7 amino acids was required. EfFull Paper
2019Decker, CE;Young, T;Pasnikowski, E;Chiu, J;Song, H;Wei, Y;Thurston, G;Daly, C;Genome-scale CRISPR activation screen uncovers tumor-intrinsic modulators of CD3 bispecific antibody efficacyScientific reports31882897Healthy donor peripheral blood mononuclear cells (PBMCs) were purchased from ReachBio Research Labs. T cells were isolated by negative selection using the Dynabeads Save;Clec12axCD3 bsAbs were isolated by selective protein A affinity chromatographyFull Paper
2019Ostrakhovitch, EA;Akakura, S;Sanokawa-Akakura, R;Tabibzadeh, S;3-Mercaptopyruvate sulfurtransferase disruption in dermal fibroblasts facilitates adipogenic trans-differentiationExperimental cell research31634482Human dermal fibroblasts (HDF) were obtained from ATCC (Manassas, VA). Mesenchymal stem cells (MSCs), isolated from a 25-year-old male donor, were purchased from ReachBio Research lab (Seattle, WA). Chemicals were from Sigma-Aldrich Company (St LouisFull Paper
2019Radtke, S;Chan, YY;Sippel, TR;Kiem, HP;Rongvaux, A;MISTRG mice support engraftment and assessment of nonhuman primate hematopoietic stem and progenitor cellsExperimental hematology30590092For CFC assays, 1000-1200 sort-purified NHP or CD34-subpopulations were seeded into 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA). Hematopoietic colonies were scored after 12-14 days. Arising colonies were identified as colony-forming unit- (CFU-) grFull Paper
2019Radtke, S;Perez, AM;Venkataraman, R;Reddy, S;Haworth, KG;Humbert, O;Kiem, HP;Peterson, CW;Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor CellsJournal of visualized experiments : JoVE30829324Protamine sulfate Sigma P-4505 14 mL Polypropylene Round-Bottom Tube Corning 352059 Colony Gel 1402 ReachBio 1402 QuadroMACS Separators Miltenyi Biotec 130-090-976Full Paper
2018Cristescu, R;Mogg, R;Ayers, M;Albright, A;Murphy, E;Yearley, J;Sher, X;Liu, XQ;Lu, H;Nebozhyn, M;Zhang, C;Lunceford, JK;Joe, A;Cheng, J;Webber, AL;Ibrahim, N;Plimack, ER;Ott, PA;Seiwert, TY;Ribas, A;McClanahan, TK;Tomassini, JE;Loboda, A;Kaufman, D;Pan-tumor genomic biomarkers for PD-1 checkpoint blockade-based immunotherapyScience30309915Associations of TMB and the T cell–inflamed GEP with BOR and PFS were evaluated by using tumor samples from subgroups of patients treated with pembrolizumab in clinical trials who had WES data available. These included a discovery cohort of patientsFull Paper
2018Smith, S;Lynch, R;Till, B;Cowan, A;Wu, Q;Voutsinas, J;Shadman, M;Blue, K;Shustov, A;Kanan, S;Low, L;Cassaday, R;Fromm, J;Gopal, A;Pembrolizumab in Combination with Standard RCHOP Therapy for Previously Untreated Diffuse Large B-Cell LymphomaBloodSecondary endpoints included response rates, overall (OS) and progression-free survival (PFS). Baseline PDL1 expression was analyzed centrally (Merck/Qualtek). Peripheral blood flow cytometry for changes in PD1, PDL-1, and PDL-2 subsets were performeFull Paper
2018Doi, T;Piha-Paul, SA;Jalal, SI;Saraf, S;Lunceford, J;Koshiji, M;Bennouna, J;Safety and Antitumor Activity of the Anti-Programmed Death-1 Antibody Pembrolizumab in Patients With Advanced Esophageal CarcinomaJ. Clin. Oncol.29116900PD-L1 Expression Analysis PD-L1 expression was assessed at screening by a central laboratory on the basis of a laboratory-developed prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA) using either an archived (formalin-fiFull Paper
2018Kanjanapan, Y;Lien, S;Yang, C;Clouthier, D;Elston, S;Jiang, H;Butler, M;Hogg, D;Ohashi, P;Pugh, T;Siu, L;Spreafico, A;Genomic and immune landscape of metastatic melanoma (MM) treated with pembrolizumab (PEM).JCOBaseline (BL) and on treatment (post 2-3 doses of PEM) fresh tumor biopsies were collected for DNA/RNA sequencing, immune-profiling, and tumor PD-L1 expression by immunohistochemistry (QualTek), with longitudinal blood collection for immunophenotypiFull Paper
2018Graff, J;Alumkal, J;Thompson, R;Moran, A;Thomas, G;Wood, M;Drake, C;Slottke, R;Beer, T;Pembrolizumab (Pembro) plus enzalutamide (Enz) in metastatic castration resistant prostate cancer (mCRPC): Extended follow up.JCOThe other 2 had neither. Of the NR who had sequencing, 4 had a DNA repair defects, and none had MSI. None of the biopsies showed tumoral PD-L1 expression (Qualtek, 22C3). Conclusions: Pembro has activity in mCRPC when added to EnzFull Paper
2018Kelly, C;Bowler, T;Munhoz, R;Chi, P;Dickson, M;Gounder, M;Keohan, M;Dholakia, R;Condy, M;Singer, S;Crago, A;Yoon, S;Ariyan, C;Hwang, S;Erinjeri, J;Antonescu, C;Qin, L;Tap, W;D'Angelo, S;A phase II study of talimogene laherparepvec (T-VEC) and pembrolizumab in patients with metastatic sarcoma.JCOoverall survival. Correlative studies included PD-L1 expression by IHC (Qualtek), multiplex IHC, characterization of tumor infiltrating lymphocytes, mutational burden/neoantigen analysis, and T cell receptor clonality. ResultsFull Paper
2018Smith, JJ;Timoshevskaya, N;Ye, C;Holt, C;Keinath, MC;Parker, HJ;Cook, ME;Hess, JE;Narum, SR;Lamanna, F;Kaessmann, H;Timoshevskiy, VA;Waterbury, CKM;Saraceno, C;Wiedemann, LM;Robb, SMC;Baker, C;Eichler, EE;Hockman, D;Sauka-Spengler, T;Yandell, M;Krumlauf, R;Elgar, G;Amemiya, CT;The sea lamprey germline genome provides insights into programmed genome rearrangement and vertebrate evolutionNat. Genet.29358652Sequencing. Fragment libraries were prepared by Covaris shearing of sperm genomic DNA isolated from a single individual and size selected to achieve average insert sizes of ~205 and 231bp. These libraries were sequenced on the Illumina HiSeq2000 platFull Paper
2018D'Angelo, SP;Melchiori, L;Merchant, MS;Bernstein, D;Glod, J;Kaplan, R;Grupp, S;Tap, WD;Chagin, K;Binder, GK;Basu, S;Lowther, DE;Wang, R;Bath, N;Tipping, A;Betts, G;Ramachandran, I;Navenot, JM;Zhang, H;Wells, DK;Van Winkle, E;Kari, G;Trivedi, T;Holdich, T;Pandite, L;Amado, R;Mackall, CL;Antitumor Activity Associated with Prolonged Persistence of Adoptively Transferred NY-ESO-1 c259T Cells in Synovial SarcomaCancer Discov29891538Serial 5-μm sections of formalin-fixed, paraffin-embedded tissues were analyzed for the following single markers by IHC: NY-ESO-1, Pan-CK (Clone AE1/AE3/PCK26, Ventana), CD45 (Clone 2B11 + PD7/26, Ventana), CD3 (Clone 2GV6, Ventana), CD8 (Clone C8/14Full Paper
2018Dejanovic, B;Huntley, MA;De Mazière, A;Meilandt, WJ;Wu, T;Srinivasan, K;Jiang, Z;Gandham, V;Friedman, BA;Ngu, H;Foreman, O;Carano, RAD;Chih, B;Klumperman, J;Bakalarski, C;Hanson, JE;Sheng, M;Changes in the Synaptic Proteome in Tauopathy and Rescue of Tau-Induced Synapse Loss by C1q AntibodiesNeuron30392797Biological Samples Human brain tissue Folio Biosciences N/AFull Paper
2018Schafer, PH;Ye, Y;Wu, L;Kosek, J;Ringheim, G;Yang, Z;Liu, L;Thomas, M;Palmisano, M;Chopra, R;Cereblon modulator iberdomide induces degradation of the transcription factors Ikaros and Aiolos: immunomodulation in healthy volunteers and relevance to systemic lupus erythematosusAnn. Rheum. Dis.29945920study in healthy volunteers. Methods. Gene expression studies. Viably frozen PBMCs from 11 patients with SLE and 10 healthy volunteers were obtained from Conversant Bio (Huntsville, Alabama, USA). Informed consent wasFull Paper
2018Liles, JT;Corkey, BK;Notte, GT;Budas, GR;Lansdon, EB;Hinojosa-Kirschenbaum, F;Badal, SS;Lee, M;Schultz, BE;Wise, S;Pendem, S;Graupe, M;Castonguay, L;Koch, KA;Wong, MH;Papalia, GA;French, DM;Sullivan, T;Huntzicker, EG;Ma, FY;Nikolic-Paterson, DJ;Altuhaifi, T;Yang, H;Fogo, AB;Breckenridge, DG;ASK1 contributes to fibrosis and dysfunction in models of kidney diseaseJ. Clin. Invest.30024858... NAL BIOPSIES AND DB/DB ENOS–/– MOUSE KIDNEYS Formalin-fixed paraffin-embedded renal biopsies from 10 patients with DKD and from 7 donors without kidney disease (“healthy”) were obtained from Folio Biosciences. Kidney sections were prepared fromFull Paper
2018Gadgeel, SM;Pennell, NA;Fidler, MJ;Halmos, B;Bonomi, P;Stevenson, J;Schneider, B;Sukari, A;Ventimiglia, J;Chen, W;Galasso, C;Wozniak, A;Boerner, J;Kalemkerian, GP;Phase II Study of Maintenance Pembrolizumab in Patients with Extensive-Stage Small Cell Lung Cancer (SCLC)J Thorac Oncol29775808than every four cycles. Retrieval of pretreatment biopsy specimens for assessment of PD-L1 expression in tumor cells and stromal tissue was conducted by Qualtek Clinical Laboratories (Newton, PA). Assessment of tumor PDFull Paper
2018Roberts, BS;Hardigan, AA;Moore, DE;Ramaker, RC;Jones, AL;Fitz-Gerald, MB;Cooper, GM;Wilcox, CM;Kimberly, RP;Myers, RM;Discovery and Validation of Circulating Biomarkers of Colorectal Adenoma by High-Depth Small RNA SequencingClin. Cancer Res.29490987HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.Full Paper
2018Tucker, DW;Getchell, CR;McCarthy, ET;Ohman, AW;Sasamoto, N;Xu, S;Ko, JY;Gupta, M;Shafrir, A;Medina, JE;Lee, JJ;MacDonald, LA;Malik, A;Hasselblatt, KT;Li, W;Zhang, H;Kaplan, SJ;Murphy, GF;Hirsch, MS;Liu, JF;Matulonis, UA;Terry, KL;Lian, CG;Dinulescu, DM;Epigenetic Reprogramming Strategies to Reverse Global Loss of 5-Hydroxymethylcytosine, a Prognostic Factor for Poor Survival in High-grade Serous Ovarian CancerClin. Cancer Res.29263182... s of 5-hmC levels in a large biorepository of HGSOC cases by using a patient tumor microarray (TMA) from the Dana-Farber Cancer Institute (DFCI, Boston, MA; ref. 22) and a commercially available TMA (Folio Biosciences). We observed strong 5-hmC sFull Paper
2018Subbiah, V;Murthy, R;Hong, DS;Prins, RM;Hosing, C;Hendricks, K;Kolli, D;Noffsinger, L;Brown, R;McGuire, M;Fu, S;Piha-Paul, S;Naing, A;Conley, AP;Benjamin, RS;Kaur, I;Bosch, ML;Cytokines Produced by Dendritic Cells Administered Intratumorally Correlate with Clinical Outcome in Patients with Diverse CancersClin. Cancer Res.30018119Biopsied tumors were formalin fixed and paraffin embedded (FFPE) using standard methods. All immunohistochemistry was performed by QualTek Molecular Laboratories (Santa Barbara, CA). In situ detection of IFNγ and TNFα transcripts in FFPE specimens wFull Paper
2018Joseph, RW;Elassaiss-Schaap, J;Kefford, R;Hwu, WJ;Wolchok, JD;Joshua, AM;Ribas, A;Hodi, FS;Hamid, O;Robert, C;Daud, A;Dronca, R;Hersey, P;Weber, JS;Patnaik, A;de Alwis, DP;Perrone, A;Zhang, J;Kang, SP;Ebbinghaus, S;Anderson, KM;Gangadhar, TC;Baseline Tumor Size Is an Independent Prognostic Factor for Overall Survival in Patients with Melanoma Treated with PembrolizumabClin. Cancer Res.29685882Tumor PD-L1 expression was assessed by a prototype IHC assay (QualTek Molecular Laboratories; ref. 14) in pretreatment tumor biopsy samples using the 22C3 antibody (Merck & Co., Inc.). PD-L1 positivity was defined as membranous staining in 1% of tumoFull Paper
2018Rugo, HS;Delord, JP;Im, SA;Ott, PA;Piha-Paul, SA;Bedard, PL;Sachdev, J;Le Tourneau, C;van Brummelen, EMJ;Varga, A;Salgado, R;Loi, S;Saraf, S;Pietrangelo, D;Karantza, V;Tan, AR;Safety and Antitumor Activity of Pembrolizumab in Patients with Estrogen Receptor-Positive/Human Epidermal Growth Factor Receptor 2-Negative Advanced Breast CancerClin. Cancer Res.29559561An archival or newly obtained core or excisional biopsy specimen from a nonirradiated tumor lesion was collected from each patient for assessment of PD-L1 expression. Tumor PD-L1 expression was assessed by immunohistochemistry at a central laboratoryFull Paper
2018Thompson, ML;Finnila, CR;Bowling, KM;Brothers, KB;Neu, MB;Amaral, MD;Hiatt, SM;East, KM;Gray, DE;Lawlor, JMJ;Kelley, WV;Lose, EJ;Rich, CA;Simmons, S;Levy, SE;Myers, RM;Barsh, GS;Bebin, EM;Cooper, GM;Genomic sequencing identifies secondary findings in a cohort of parent study participantsGenet. Med.29790872to change their preferences at the return of results appointment. Whole exome and genome sequencing Blood samples were sent for sequencing at the HudsonAlpha Genomic Services Laboratory (http://gsl.hudsonalpha.org). Genomic DNA was isolated from periFull Paper
2018Pena, LDM;Jiang, YH;Schoch, K;Spillmann, RC;Walley, N;Stong, N;Rapisardo Horn, S;Sullivan, JA;McConkie-Rosell, A;Kansagra, S;Smith, EC;El-Dairi, M;Bellet, J;Keels, MA;Jasien, J;Kranz, PG;Noel, R;Nagaraj, SK;Lark, RK;Wechsler, DSG;Del Gaudio, D;Leung, ML;Hendon, LG;Parker, CC;Jones, KL;, ;Goldstein, DB;Shashi, V;Looking beyond the exome: a phenotype-first approach to molecular diagnostic resolution in rare and undiagnosed diseasesGenet. Med.28914269Kimberly Splinter Harvard University (CC) David P. Bick HudsonAlpha Camille L. Birch HudsonAlpha Braden E. Boone HudsonAlpha Donna M. Brown HudsonAlpha Daniel C. Dorset HudsonAlpha Lori H. Handley HudsonAlpha Howard J. Jacob HudsonAlpha Angela L. JoFull Paper
2018Abraham, B;Pharma, GUCB;Sankyo, HD;Disclosure of relevant financial relationshipsJournal of the American Academy of DermatologyAbramovits, William; Grants - AbbVie, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant Bio, Dermavant Abramovits, William; Honoraria - AbbVie, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant Bio, DFull Paper
2018Menard, LC;Fischer, P;Kakrecha, B;Linsley, PS;Wambre, E;Liu, MC;Rust, BJ;Lee, D;Penhallow, B;Manjarrez Orduno, N;Nadler, SG;Renal Cell Carcinoma (RCC) Tumors Display Large Expansion of Double Positive (DP) CD4+CD8+ T Cells With Expression of Exhaustion MarkersFront Immunol30534127Samples for the discovery and replication cohorts were provided by Folio-Conversant Bio (AL), MT Group (CA), Rutgers-CINJ or the Benaroya Research Institute. All patients gave written informed consent at time of sample collection according to IRB proFull Paper
2018Tisdale, J;Kanter, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Schmidt, M;Miller, A;Pierciey, F;Shi, W;Ribeil, J;Asmal, M;Thompson, A;Walters, M;Current Results of Lentiglobin Gene Therapy in Patients with Severe Sickle Cell Disease Treated Under a Refined Protocol in the Phase 1 Hgb-206 StudyBloodBackground β-globin gene transfer has the potential for substantial clinical benefit in patients with sickle cell disease (SCD). LentiGlobin Drug Product (DP) contains autologous CD34+ hematopoietic stem cells (HSCs) transduced with thFull Paper
2018Klemann, CJHM;Xicoy, H;Poelmans, G;Bloem, BR;Martens, GJM;Visser, JE;Physical Exercise Modulates L-DOPA-Regulated Molecular Pathways in the MPTP Mouse Model of Parkinson's DiseaseMol. Neurobiol.29019056pooled for RNAseq analysis. RNA Sequencing and Data Processing. All RNA samples were subjected to RNA sequencing (RNAseq; HudsonAlpha Genomic Services Lab, Huntsville, AL) as performed before [37]. In short, totalFull Paper
2018Van Laar, R;Lincoln, M;Van Laar, B;Development and validation of a plasma-based melanoma biomarker suitable for clinical useBr. J. Cancer29360813expression profiles of individuals with and without melanoma, 0.5-2 ml of cell-free plasma was obtained from 16 healthy controls and 32 Caucasian individuals at the time of their diagnosis with cutaneous melanoma (Cureline Inc., CA, USA and Folio BioFull Paper
2018Buatois, V;Johnson, Z;Salgado-Pires, S;Papaioannou, A;Hatterer, E;Chauchet, X;Richard, F;Barba, L;Daubeuf, B;Cons, L;Broyer, L;D'Asaro, M;Matthes, T;LeGallou, S;Fest, T;Tarte, K;Clarke Hinojosa, RK;Genescà Ferrer, E;Ribera, JM;Dey, A;Bailey, K;Fielding, AK;Eissenberg, L;Ritchey, J;Rettig, M;DiPersio, JF;Kosco-Vilbois, MH;Masternak, K;Fischer, N;Shang, L;Ferlin, WG;Preclinical Development of a Bispecific Antibody that Safely and Effectively Targets CD19 and CD47 for the Treatment of B-Cell Lymphoma and LeukemiaMol. Cancer Ther.29743205Cells were cultured at 37°C and 5% CO2. Primary samples were collected from Conversant Biologics (Huntsville, USA), the Leukemia Research Center (Glasgow, UK), the Geneva University Hospital (Geneva, Switzerland) ;... tometry and finally selecteFull Paper
2018Foulk, B;Schaffer, M;Gross, S;Rao, C;Smirnov, D;Connelly, MC;Chaturvedi, S;Reddy, M;Brittingham, G;Mata, M;Repollet, M;Rojas, C;Auclair, D;DeRome, M;, ;Weiss, B;Sasser, AK;Enumeration and characterization of circulating multiple myeloma cells in patients with plasma cell disordersBr. J. Haematol.29105742Verification cohort samples were obtained from Conversant Biologics (Huntsville, AL, USA) under a biospecimen purchase agreement. These samples represent a heterogenous cohort of patients with active disease and those in remissionFull Paper
2018Pekcec, A;Schülert, N;Stierstorfer, B;Deiana, S;Dorner-Ciossek, C;Rosenbrock, H;Targeting the dopamine D1 receptor or its downstream signalling by inhibiting phosphodiesterase-1 improves cognitive performanceBr. J. Pharmacol.29726015paraffin blocks. Human brain tissue from healthy donors (Folio Biosciences, Ohio, USA) was fixed in 4% paraformaldehyde, and samples containing PFC were identified, trimmed and processed into paraffin blocks. All procurementFull Paper
2018Maity, A;Mick, R;Huang, AC;George, SM;Farwell, MD;Lukens, JN;Berman, AT;Mitchell, TC;Bauml, J;Schuchter, LM;O'Hara, M;Lin, LL;Demichele, A;Christodouleas, JP;Haas, NB;Patsch, DM;Hahn, SM;Minn, AJ;Wherry, EJ;Vonderheide, RH;A phase I trial of pembrolizumab with hypofractionated radiotherapy in patients with metastatic solid tumoursBr. J. Cancer30318516ange in volume of the irradiated (index) lesion was also calculated, but this was not used in the RECIST measurement. Immunohistochemical staining PD-L1 staining was performed by an outside company (QualTek Molecular Laboratories, Newtown, PA, USA)Full Paper
2018Mehra, R;Seiwert, TY;Gupta, S;Weiss, J;Gluck, I;Eder, JP;Burtness, B;Tahara, M;Keam, B;Kang, H;Muro, K;Geva, R;Chung, HC;Lin, CC;Aurora-Garg, D;Ray, A;Pathiraja, K;Cheng, J;Chow, LQM;Haddad, R;Efficacy and safety of pembrolizumab in recurrent/metastatic head and neck squamous cell carcinoma: pooled analyses after long-term follow-up in KEYNOTE-012Br. J. Cancer29955135PD-L1 expression status during screening was determined using a prototype PD-L1 immunohistochemical assay 18 performed at a laboratory site (QualTek) accredited by the College of American Pathologists and Clinical Laboratory Improvement Amendments, aFull Paper
2018Wein, L;Luen, SJ;Savas, P;Salgado, R;Loi, S;Checkpoint blockade in the treatment of breast cancer: current status and future directionsBr. J. Cancer29808015Patients were required to be PD-L1 positive by immunohistochemistry (IHC), which was defined as PD-L1 positivity in the stroma or ≥1% tumour cells using their Qualtek assay.28 Most patients were heavily pre-treated; the median number of previous lineFull Paper
2018Kanter, J;Tisdale, J;Kwiatkowski, J;Krishnamurti, L;Mapara, M;Schmidt, M;Miller, A;Pierciey, F;Shi, W;Ribeil, J;Walters, M;Thompson, A;Outcomes for Initial Patient Cohorts with up to 33 Months of Follow-up in the Hgb-206 Phase 1 TrialBloodBackground Sickle cell disease (SCD) is a progressively debilitating genetic disease causing significant morbidity and early mortality for which a universal curative therapy is lacking. Expression of an anti-sickling β-globin via geneFull Paper
2018Bosco, EE;Christie, RJ;Carrasco, R;Sabol, D;Zha, J;DaCosta, K;Brown, L;Kennedy, M;Meekin, J;Phipps, S;Ayriss, J;Du, Q;Bezabeh, B;Chowdhury, P;Breen, S;Chen, C;Reed, M;Hinrichs, M;Zhong, H;Xiao, Z;Dixit, R;Herbst, R;Tice, DA;Preclinical evaluation of a GFRA1 targeted antibody-drug conjugate in breast cancerOncotarget29796165... Fisher Scientific, Waltham, MA, USA) were coated with 5 mg/ml 9B3 antibody at 4○C overnight, washed, and blocked with 1% bovine serum albumin (Sigma-Aldrich, St Louis, MO). Human serum samples from Conversant Bio (Huntsville, AL) or from cell-linFull Paper
2018Xu, Z;Wang, S;Li, Y;Zhu, F;Huang, J;PRIM: An Efficient Preconditioning Iterative Reweighted Least Squares Method for Parallel Brain MRI ReconstructionNeuroinformatics29423650International Holdings Inc. Albin, Mary, Westinghouse Alexander, William, Qualtek Manufacturing, Inc. Alexander, Chuck, Solid Concepts, Inc. Alexopoulos, Nick, Broadcom Corporation Alford, Charles, UES, Inc. Alvarado, IgorFull Paper
2018Locatelli, F;Walters, M;Kwiatkowski, J;Porter, J;Sauer, M;Thuret, I;Hongeng, S;Kulozik, A;Lal, A;Thrasher, A;Yannaki, E;Elliot, H;Tao, G;Asmal, M;Thompson, A;Lentiglobin Gene Therapy for Patients with Transfusion-Dependent β-Thalassemia (TDT): Results from the Phase 3 Northstar-2 and Northstar-3 StudiesBloodBackground Transfusion-dependent β-thalassemia (TDT) is a severe genetic disease caused by impaired β-globin production, leading to severe anemia, lifelong transfusion dependence with iron overload and serious comorbidities. Gene theraFull Paper
2018Li, C;Mathews, V;George, B;Kim, S;Hebert, K;Jiang, H;Li, C;Zhu, Y;Keesler, D;Agarwal, R;Boelens, J;Dvorak, C;Auletta, J;Goyal, R;Hanna, R;Kasow, K;Margolis, D;Shenoy, S;Walters, M;Eapen, M;Related and Unrelated Donor Transplantation for β Thalassemia Major: Results of an International SurveyBloodWe studied 1110 children, adolescents and young adults with β thalassemia major transplanted with grafts from HLA-matched relative (n=677; 61%), HLA-mismatched relative (n=78; 7%), HLA-matched unrelated (n=252; 23%) and HLA-mismatched unrelated (n=10Full Paper
2018Rasko, J;Thompson, A;Kwiatkowski, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;Schmidt, M;Kletzel, M;Leboulch, P;Vichinsky, E;Deary, B;Chen, Y;Asmal, M;Walters, M;Clinical Outcomes of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia Following Completion of the Northstar HGB-204 StudyBloodBackground Advances in red blood cell (RBC) transfusion and chelation have improved the prognosis of patients with transfusion-dependent β-thalassemia (TDT); however, many patients experience organ damage due to iron overload and otherFull Paper
2018Godwin, RL;Opatova, V;Garrison, NL;Hamilton, CA;Bond, JE;Phylogeny of a cosmopolitan family of morphologically conserved trapdoor spiders (Mygalomorphae, Ctenizidae) using Anchored Hybrid Enrichment, with a description of the family, Halonoproctidae Pocock 1901Mol. Phylogenet. Evol.29656103Phylogeny of a cosmopolitan family of morphologically conserved trapdoor spiders (Mygalomorphae, Ctenizidae) using Anchored Hybrid Enrichment, with a description of the family, Halonoproctidae Pocock 1901Full Paper
2018Hansen, R;Firdaus, SJ;Li, S;Janes, MR;Zhang, J;Liu, Y;Zarrinkar, PP;An Internally Controlled Quantitative Target Occupancy Assay for Covalent InhibitorsSci Rep30254226spectrometry. Clinical specimens. Clinical FFPE specimens were purchased from Conversant Bio (Huntsville, AL) and Asterand Bioscience (Detroit, MI). Conversant and Asterand comply with all applicable regulations. Animal studiesFull Paper
2018Potts, AH;Leng, Y;Babitzke, P;Romeo, T;Examination of Csr regulatory circuitry using epistasis analysis with RNA-seq (Epi-seq) confirms that CsrD affects gene expression via CsrA, CsrB and CsrCSci Rep29599472RNA-seq libraries were then generated with the Stranded RNA-Seq Library Preparation Kit for Illumina (KAPA) and NEBNext Multiplex Oligos for Illumina adaptors (NEB) according to the manufacturer’s instructions for 100 ng of starting material and a meFull Paper
2018Friedman, KM;Garrett, TE;Evans, JW;Horton, HM;Latimer, HJ;Seidel, SL;Horvath, CJ;Morgan, RA;Effective Targeting of Multiple B-Cell Maturation Antigen-Expressing Hematological Malignances by Anti-B-Cell Maturation Antigen Chimeric Antigen Receptor T CellsHum. Gene Ther.29641319LLC. Peripheral blood mononuclear cells (PBMCs) were isolated and cryopreserved in the authors' laboratory. Whole blood from two CLL patients was obtained from Conversant Biologics, Inc. Immunohistochemistry. TwentyFull Paper
2018Vasanthavel, S;Meenakshi, K;Nivedha, V;Ballamurugan, AM;Kannan, S;Tuning the structural and mechanical properties in ZrO2-SiO2 binary system through Y3+ inclusionsMater Sci Eng C Mater Biol Appl29519433Zirconia (ZrO 2 ) and Alumina (Al 2 O 3 ) are conversant bio-inert ceramics known for their superior mechanical properties while hydroxyapatite [Ca 5 (PO 4 ) 3 OH, HAP], β-tricalciumphosphate [β-Ca 3 (PO 4 ) 2 , β-TCP] and SiO 2 based bioactive glassFull Paper
2018Schlegel, C;Lapierre, LA;Weis, VG;Williams, JA;Kaji, I;Pinzon-Guzman, C;Prasad, N;Boone, B;Jones, A;Correa, H;Levy, SE;Han, X;Wang, M;Thomsen, K;Acra, S;Goldenring, JR;Reversible deficits in apical transporter trafficking associated with deficiency in diacylglycerol acyltransferaseTraffic30095213Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee.++Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee.++Department of Surgery, Vanderbilt University School of Medicine, NashvilleFull Paper
2018Selvan, N;George, S;Serajee, FJ;Shaw, M;Hobson, L;Kalscheuer, V;Prasad, N;Levy, SE;Taylor, J;Aftimos, S;Schwartz, CE;Huq, AM;Gecz, J;Wells, L;O-GlcNAc transferase missense mutations linked to X-linked intellectual disability deregulate genes involved in cell fate determination and signalingJ. Biol. Chem.29769320From the Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602.++From the Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, UniversitFull Paper
2018Edwardson, CF;Hollibaugh, JT;Composition and Activity of Microbial Communities along the Redox Gradient of an Alkaline, Hypersaline, LakeFront Microbiol29445359Samples were pooled and sequenced on one lane of an Illumina HiSeq2500 in Rapid Run mode with the 150PE (300 cycle) kit at HudsonAlpha Genomic Services Lab (Huntsville, AL). Bioinformatics of 16S rRNA SequencesFull Paper
2018Xu, J;Su, X;Bonizzoni, M;Zhong, D;Li, Y;Zhou, G;Nguyen, H;Tong, S;Yan, G;Chen, XG;Comparative transcriptome analysis and RNA interference reveal CYP6A8 and SNPs related to pyrethroid resistance in Aedes albopictusPLoS Negl Trop Dis30418967; RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). A total of 24 cDNA libraries were prepared individually from 12 susceptible and 12 resistant mosquFull Paper
2018Biase, FH;Kimble, KM;Functional signaling and gene regulatory networks between the oocyte and the surrounding cumulus cellsBMC Genomics29747587The samples were subjected to 16 cycles of PCR amplification. For all samples, we used 1 ng of amplified cDNA for library preparation with the Nextera XT DNA library preg Kit (Illumina, Inc.), as per the procedures described in the SMART-seq2 protocoFull Paper
2018Dickinson, SE;Griffin, BA;Elmore, MF;Kriese-Anderson, L;Elmore, JB;Dyce, PW;Rodning, SP;Biase, FH;Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potentialBMC Genomics29426285Libraries were prepared with the TruSeq Stranded mRNA Library Prep kit (Illumina, Inc., San Diego, CA) following manufacturer’s instructions. Libraries were quantified with Qubit™ dsDNA High Sensitivity Assay Kit (Eurogene, OR) and quality was evaluaFull Paper
2018Lin, EM;Gong, J;Klempner, SJ;Chao, J;Advances in immuno-oncology biomarkers for gastroesophageal cancer: Programmed death ligand 1, microsatellite instability, and beyondWorld J. Gastroenterol.29991874Presence of tumor PD-L1 expression was a requirement for enrollment, with 40% of the patients screened demonstrating PD-L1-positive tumors. PD-L1 expression was detected using the 22C3 antibody with a prototype assay using QualTek or Dako platformsFull Paper
2018Fan, J;Lerner, J;Wyatt, MK;Cai, P;Peterson, K;Dong, L;Wistow, G;The klotho-related protein KLPH (lctl) has preferred expression in lens and is essential for expression of clic5 and normal lens suture formationExp. Eye Res.29425878XAR film at −70 °C. 2.3. In situ hybridization (ISH). Frozen tissues were cut into 10 μm sections, mounted on gelatin-coated slides and stored at −80 °C. ISH was performed at Phylogeny Inc. (Columbus, OH). Slides were fixed inFull Paper
2018Hiatt, SM;Amaral, MD;Bowling, KM;Finnila, CR;Thompson, ML;Gray, DE;Lawlor, JMJ;Cochran, JN;Bebin, EM;Brothers, KB;East, KM;Kelley, WV;Lamb, NE;Levy, SE;Lose, EJ;Neu, MB;Rich, CA;Simmons, S;Myers, RM;Barsh, GS;Cooper, GM;Systematic reanalysis of genomic data improves quality of variant interpretationClin. Genet.29652076HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.Full Paper
2018Håvik, AL;Bruland, O;Aarhus, M;Kalland, KH;Stokowy, T;Lund-Johansen, M;Knappskog, PM;Screening for viral nucleic acids in vestibular schwannomaJ. Neurovirol.30168016; RNA sequencing on RNA extracted from five sVS tissue biopsies was performed by HudsonAlpha (HudsonAlpha Genomic Services Laboratory, 601 Genome Way, Huntsville, AL 35806 USA) using their "Poly A RNA-sequencing" service including 50 cycles of paireFull Paper
2018Mohan, S;Greenstein, I;Ng, C;Frazier, K;Nguyen, G;Harding, L;Barlow, D;Assessing N w-hydroxy-L-arginine applicability as a novel ethnic specific estrogen-negative breast cancer markerAmino Acids29260322Collection (Manassas, VA). We received de-identified plasma samples from Cooperative Human Tissue Network (Philadelphia, PA), Tissue-for-Research (Atlanta, GA), and Conversant Bio (Huntsville, AL). The de-identifiedFull Paper
2018Zhu, J;Gan, X;Ao, Q;Shen, X;Tan, Y;Chen, M;Luo, Y;Wang, H;Jiang, H;Li, C;Basal polarization of the immune responses to Streptococcus agalactiae susceptible and resistant tilapia (Oreochromis niloticus)Fish Shellfish Immunol.29454032; For each timepoint, equal amounts of RNA from the three replicates were pooled for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA)Full Paper
2018Porter, ET;Porter, FS;A Strain Gauge Monitor (SGM) for Continuous Valve Gape Measurements in Bivalve Molluscs in Response to Laboratory Induced Diel-cycling Hypoxia and pHJ Vis Exp30124658Name Company Catalog Number Comments Campbell CR 10x data logger Campbell Scientific, Logan, Utah Or other data logger. At Campbell the CR 10X has been replaced with the CR 1000 Campbell CR 10x multiplexer Campbell Scientific, Logan, Utah Data loggFull Paper
2018Yang, Y;Fu, Q;Liu, Y;Wang, X;Dunham, R;Liu, S;Bao, L;Zeng, Q;Zhou, T;Li, N;Qin, Z;Jiang, C;Gao, D;Liu, Z;Comparative transcriptome analysis reveals conserved branching morphogenesis related genes involved in chamber formation of catfish swimbladderPhysiol. Genomics29167198Equal amounts of RNA from the three replicates for each sample were pooled together for RNA-Seq library preparation. The remaining replicate pools were preserved as biological replicates for quantitative (q)RT-PCR validation. The cDNA libraries wereFull Paper
2018Cohen, RB;Delord, JP;Doi, T;Piha-Paul, SA;Liu, SV;Gilbert, J;Algazi, AP;Damian, S;Hong, RL;Le Tourneau, C;Day, D;Varga, A;Elez, E;Wallmark, J;Saraf, S;Thanigaimani, P;Cheng, J;Keam, B;Pembrolizumab for the Treatment of Advanced Salivary Gland Carcinoma: Findings of the Phase 1b KEYNOTE-028 StudyAm. J. Clin. Oncol.29462123Tumor PD-L1 positivity was determined at a central laboratory using formalin-fixed archived or newly obtained biopsy samples and a prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA),17 based on the 22C3 antibody (Merck &Full Paper
2018Glover, ME;McCoy, CR;Shupe, EA;Unroe, KA;Jackson, NL;Clinton, SM;Perinatal exposure to the SSRI paroxetine alters the methylome landscape of the developing dentate gyrusEur. J. Neurosci.30585666; quantified (Quant-iT high sensitivity DNA Assay Kit, Invitrogen, Grand Island, NY). Next- generation sequencing was performed at HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) using NEBNext reagents (New EnglaFull Paper
2018Verwimp, C;Ruttink, T;Muylle, H;Van Glabeke, S;Cnops, G;Quataert, P;Honnay, O;Roldán-Ruiz, I;Temporal changes in genetic diversity and forage yield of perennial ryegrass in monoculture and in combination with red clover in swardsPLoS ONE30408053The protocol for the 112 tissue pool samples was slightly adjusted, based on the results of the validation step. In this case, the 50 μL bead-purified ligate was split into three aliquots, and 16.7 μL was used for three replicate PCR reactions to accFull Paper
2018Geister, KA;Timms, AE;Beier, DR;Optimizing Genomic Methods for Mapping and Identification of Candidate Variants in ENU Mutagenesis Screens Using Inbred MiceG3 (Bethesda)29208648... centrifugation. DNA was tested at SCRI for purity and quality using a Nanodrop spectrophotometer and the Qubit dsDNA Broad Range Assay (#Q32850; Thermo Fisher Scientific). DNA samples were shipped to HudsonAlpha Genomic Services Laboratory (HuntsFull Paper
2018Papanek, B;O'Dell, KB;Manga, P;Giannone, RJ;Klingeman, DM;Hettich, RL;Brown, SD;Guss, AM;Transcriptomic and proteomic changes from medium supplementation and strain evolution in high-yielding Clostridium thermocellum strainsJ. Ind. Microbiol. Biotechnol.30187243; Sequencing was completed using a SR50 sequencing protocol on an Illumina HiSeq 2500 platform (HudsonAlpha Genomic Services Laboratory; Huntsville, AL, USA). RNA-Seq analysis. The analysis of the raw RNA-Seq data was performed as previously describFull Paper
2018Ziai, J;Gilbert, HN;Foreman, O;Eastham-Anderson, J;Chu, F;Huseni, M;Kim, JM;CD8+ T cell infiltration in breast and colon cancer: A histologic and statistical analysisPLoS ONE29320521IV were represented. Colorectal cancer samples were procured from Conversant Bio and included moderately differentiated adenocarcinoma (9), mucinous carcinoma (1) and rectal (2) carcinoma cases. Samples containedFull Paper
2018Gallery, M;Zhang, J;Bradley, DP;Brauer, P;Cvet, D;Estevam, J;Danaee, H;Greenfield, E;Li, P;Manfredi, M;Loke, HK;Rabino, C;Stringer, B;Williamson, M;Wyant, T;Yang, J;Zhu, Q;Abu-Yousif, A;Veiby, OP;A monomethyl auristatin E-conjugated antibody to guanylyl cyclase C is cytotoxic to target-expressing cells in vitro and in vivoPLoS ONE29370189scanning system. Colon tissue microarrays (US Biomax) were stained and scored by two independent pathologists following a protocol developed at Qualtek Molecular Laboratories on their Tek-Mate automated stainer. Briefly ;ith hematoxylin and imagFull Paper
2018Lagatie, O;Verheyen, A;Nijs, E;Van Dorst, B;Batsa Debrah, L;Debrah, A;Supali, T;Sartono, E;Stuyver, LJ;Evaluation of the Diagnostic Performance of Onchocerca volvulus Linear Epitopes in a Peptide Enzyme-Linked Immunosorbent AssayAm. J. Trop. Med. Hyg.29313477(Glasgow, Scotland). A sec- ond healthy control sample set was composed of 49 plasma samples from healthy individuals from Belgium.20-24 Further- more, different sets of plasma samples were obtained from Discovery Life Sciences, IncFull Paper
2018Andreotta, PW;Arold, S;Kenyon, J;Spicer, D;Woodman, P;Berry, E;Brogan, T;Kong, S;Okerholm, P;Russell, V;Clarke, RW;Hava, DL;Inhaled calcium salts inhibit tobacco smoke-induced inflammation by modulating expression of chemokines and cytokinesPulm Pharmacol Ther30359782Secreted protein expression and gene expression were assayed 2 h after LPS stimulation. 2.5. Human macrophage cultures. Human CD14 + monocytes were purchased from Conversant Biologics and differentiated into macrophages in cultureFull Paper
2018Fall, L;Clevenger, J;McGregor, C;Assay development and marker validation for marker assisted selection of Fusarium oxysporum f. sp. niveum race 1 in watermelonMol Breedingfor sequencing. Library construction and whole genome Illumina sequencing on an Illumina HiSeq X (Illumina, Inc., San Diego, CA) were performed at HudsonAlpha Genomic services laboratory (Huntsville, AL). ConstructedFull Paper
2018Zobel-Thropp, PA;Bulger, EA;Cordes, MHJ;Binford, GJ;Gillespie, RG;Brewer, MS;Sexually dimorphic venom proteins in long-jawed orb-weaving spiders (Tetragnatha) comprise novel gene familiesPeerJ29876146RNA extractions from the BF population of T. versicolor males (two) and females (10) were shipped to the Genomic Services Lab at HudsonAlpha (Huntsville, AL, USA), where cDNA libraries were prepared from total RNA (poly-A isolation, Illumina TruSeq RFull Paper
2018Awad, H;Bratasz, A;Nuovo, G;Burry, R;Meng, X;Kelani, H;Brown, M;Ramadan, ME;Williams, J;Bouhliqah, L;Popovich, PG;Guan, Z;Mcallister, C;Corcoran, SE;Kaspar, B;Michele Basso, D;Otero, JJ;Kirsch, C;Davis, IC;Croce, CM;Michaille, JJ;Tili, E;MiR-155 deletion reduces ischemia-induced paralysis in an aortic aneurysm repair mouse model: Utility of immunohistochemistry and histopathology in understanding etiology of spinal cord paralysisAnn Diagn Pathol29966831The degree of permeability was determined by measuring the fluorescence of the receiver plate well solution. 2.5. MicroRNA in situ hybridization (ISH). ISH for miR-155 was performed by Phylogeny Inc. (Powell, OH), as previously described [16]Full Paper
2018Dufu, K;Patel, M;Oksenberg, D;Cabrales, P;GBT440 improves red blood cell deformability and reduces viscosity of sickle cell blood under deoxygenated conditionsClin. Hemorheol. Microcirc.29660913The study was approved by the IRB of the University of North Carolina (UNC) Sickle Cell program [IRB # 88-0343 (GCRC-101)]. Blood from trait individuals (AS) collected in sodium citrate vacutainers was purchased from Conversant Bio, Inc. (Huntsville,Full Paper
2018Smyth, E;Thuss-Patience, PC;Immune Checkpoint Inhibition in Gastro-Oesophageal CancerOncol Res Treat29705787gastro-oesophageal cancer. Unlike the studies with nivolumab, in the KEYNOTE- 12 patients were selected for PD-L1-positive status; however, a different antibody (QualTek 22C3) and scoring system were used. Eligibility wasFull Paper
2018Yuan, Z;Huang, W;Liu, S;Xu, P;Dunham, R;Liu, Z;Historical demography of common carp estimated from individuals collected from various parts of the world using the pairwise sequentially markovian coalescent approachGenetica29299799; Qiagen). Genome re-sequencing and sequence alignment. DNA library preparation and sequencing were conducted at HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA) following manufacturer's instructionsFull Paper
2018Vakulskas, CA;Dever, DP;Rettig, GR;Turk, R;Jacobi, AM;Collingwood, MA;Bode, NM;McNeill, MS;Yan, S;Camarena, J;Lee, CM;Park, SH;Wiebking, V;Bak, RO;Gomez-Ospina, N;Pavel-Dinu, M;Sun, W;Bao, G;Porteus, MH;Behlke, MA;A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cellsNature medicine30082871ere used from either fresh cord blood (CB) (kindly provided by Binns Family program for Cord Blood Research), isolated from the peripheral blood of SCD patients, or frozen plerixafor mobilized CD34+ (AllCells). HSPCs were cultured at 250,000-500,000Full Paper
2018Good, Z;Sarno, J;Jager, A;Samusik, N;Aghaeepour, N;Simonds, EF;White, L;Lacayo, NJ;Fantl, WJ;Fazio, G;Gaipa, G;Biondi, A;Tibshirani, R;Bendall, SC;Nolan, GP;Davis, KL;Single-cell developmental classification of B cell precursor acute lymphoblastic leukemia at diagnosis reveals predictors of relapseNature medicine29505032Reagents and methods relevant to this work can also be found in the Life Sciences Reporting Summary. Fresh human bone marrow was obtained from healthy adult donors (n = 5; 3 females: 20, 27, 44 y.o., 2 males: 26, 28 y.o., average: 29 y.o.; AllCells,Full Paper
2018Deng, M;Gui, X;Kim, J;Xie, L;Chen, W;Li, Z;He, L;Chen, Y;Chen, H;Luo, W;Lu, Z;Xie, J;Churchill, H;Xu, Y;Zhou, Z;Wu, G;Yu, C;John, S;Hirayasu, K;Nguyen, N;Liu, X;Huang, F;Li, L;Deng, H;Tang, H;Sadek, AH;Zhang, L;Huang, T;Zou, Y;Chen, B;Zhu, H;Arase, H;Xia, N;Jiang, Y;Collins, R;You, MJ;Homsi, J;Unni, N;Lewis, C;Chen, GQ;Fu, YX;Liao, XC;An, Z;Zheng, J;Zhang, N;Zhang, CC;LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltrationNature30333625ockout was validated by flow cytometry. Leukemia cell and T cell co-culture assay In the co-culture assay, human T cells (5 × 104 per well) isolated from health donor peripheral blood (PB009-1-0, Allcells) were mixed with irradiated (28 Gy) indicateFull Paper
2018Roth, TL;Puig-Saus, C;Yu, R;Shifrut, E;Carnevale, J;Li, PJ;Hiatt, J;Saco, J;Krystofinski, P;Li, H;Tobin, V;Nguyen, DN;Lee, MR;Putnam, AL;Ferris, AL;Chen, JW;Schickel, JN;Pellerin, L;Carmody, D;Alkorta-Aranburu, G;Del Gaudio, D;Matsumoto, H;Morell, M;Mao, Y;Cho, M;Quadros, RM;Gurumurthy, CB;Smith, B;Haugwitz, M;Hughes, SH;Weissman, JS;Schumann, K;Esensten, JH;May, AP;Ashworth, A;Kupfer, GM;Greeley, SAW;Bacchetta, R;Meffre, E;Roncarolo, MG;Romberg, N;Herold, KC;Ribas, A;Leonetti, MD;Marson, A;Reprogramming human T cell function and specificity with non-viral genome targetingNature29995861Additional leukapheresis products from healthy donors were collected either under UCLA Institutional Review Board (IRB) approval #10-001598 or purchased from AllCells, LLC. All SaveFull Paper
2018Butler, A;Hoffman, P;Smibert, P;Papalexi, E;Satija, R;Integrating single-cell transcriptomic data across different conditions, technologies, and speciesNature biotechnology29608179We therefore repeated the original experiment, where PBMCs from a healthy human donor (ALLCELLS) were cultured with RPMI medium supplemented with 10% FBS and stimulated SaveFull Paper
2018Neal, JT;Li, X;Zhu, J;Giangarra, V;Grzeskowiak, CL;Ju, J;Liu, IH;Chiou, SH;Salahudeen, AA;Smith, AR;Deutsch, BC;Liao, L;Zemek, AJ;Zhao, F;Karlsson, K;Schultz, LM;Metzner, TJ;Nadauld, LD;Tseng, YY;Alkhairy, S;Oh, C;Keskula, P;Mendoza-Villanueva, D;De La Vega, FM;Kunz, PL;Liao, JC;Leppert, JT;Sunwoo, JB;Sabatti, C;Boehm, JS;Hahn, WC;Zheng, GXY;Davis, MM;Kuo, CJ;Organoid Modeling of the Tumor Immune MicroenvironmentCell30550791PBMCs, Pan T and CD19 + B cells used for the technical validation part of the immune profiling solution were obtained from AllCells (Catalog #: PB001, PB009-1F and PB010-0). Tumor SaveFull Paper
2018Buenrostro, JD;Corces, MR;Lareau, CA;Wu, B;Schep, AN;Aryee, MJ;Majeti, R;Chang, HY;Greenleaf, WJ;Integrated Single-Cell Analysis Maps the Continuous Regulatory Landscape of Human Hematopoietic DifferentiationCell29706549Healthy adult bone marrow allcells https://www.allcells.com/products/whole-bone-marrow- Samples were collected commercially from allcells. Further information about the donors SaveFull Paper
2018Su, R;Dong, L;Li, C;Nachtergaele, S;Wunderlich, M;Qing, Y;Deng, X;Wang, Y;Weng, X;Hu, C;Yu, M;Skibbe, J;Dai, Q;Zou, D;Wu, T;Yu, K;Weng, H;Huang, H;Ferchen, K;Qin, X;Zhang, B;Qi, J;Sasaki, AT;Plas, DR;Bradner, JE;Wei, M;Marcucci, G;Jiang, X;Mulloy, JC;Jin, J;He, C;Chen, J;R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA SignalingCell29249359R-2-hydroxyglutarate (R-2HG), produced at high levels by mutant isocitrate dehydrogenase 1/2 (IDH1/2) enzymes, was reported as an oncometabolite. We show here that R-2HG also SaveFull Paper
2018Choi, HW;Suwanpradid, J;Kim, IH;Staats, HF;Haniffa, M;MacLeod, AS;Abraham, SN;Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesiclesScience (New York, N.Y.)30409859100 U/ml penicillin, and 100 μg/ml streptomycin was utilized. In brief, purified peripheral blood CD34+ peripheral blood cells (Allcells) were suspended at 5×105 cells/ml in supplemented IMDM containing 100 ng/ml recombinant human (rh) SCF, 10 ng/mlFull Paper
2018Ting, PY;Parker, AE;Lee, JS;Trussell, C;Sharif, O;Luna, F;Federe, G;Barnes, SW;Walker, JR;Vance, J;Gao, MY;Klock, HE;Clarkson, S;Russ, C;Miraglia, LJ;Cooke, MP;Boitano, AE;McNamara, P;Lamb, J;Schmedt, C;Snead, JL;Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cellsNature methods30297964AllCells and Lonza also obtained samples from outside sources with documented IRB compliance. Lot numbers can be found in the Nature Research Reporting Summary. SaveFull Paper
2018Jenkins, RW;Aref, AR;Lizotte, PH;Ivanova, E;Stinson, S;Zhou, CW;Bowden, M;Deng, J;Liu, H;Miao, D;He, MX;Walker, W;Zhang, G;Tian, T;Cheng, C;Wei, Z;Palakurthi, S;Bittinger, M;Vitzthum, H;Kim, JW;Merlino, A;Quinn, M;Venkataramani, C;Kaplan, JA;Portell, A;Gokhale, PC;Phillips, B;Smart, A;Rotem, A;Jones, RE;Keogh, L;Anguiano, M;Stapleton, L;Jia, Z;Barzily-Rokni, M;Cañadas, I;Thai, TC;Hammond, MR;Vlahos, R;Wang, ES;Zhang, H;Li, S;Hanna, GJ;Huang, W;Hoang, MP;Piris, A;Eliane, JP;Stemmer-Rachamimov, AO;Cameron, L;Su, MJ;Shah, P;Izar, B;Thakuria, M;LeBoeuf, NR;Rabinowits, G;Gunda, V;Parangi, S;Cleary, JM;Miller, BC;Kitajima, S;Thummalapalli, R;Miao, B;Barbie, TU;Sivathanu, V;Wong, J;Richards, WG;Bueno, R;Yoon, CH;Miret, J;Herlyn, M;Garraway, LA;Van Allen, EM;Freeman, GJ;Kirschmeier, PT;Lorch, JH;Ott, PA;Hodi, FS;Flaherty, KT;Kamm, RD;Boland, GM;Wong, KK;Dornan, D;Paweletz, CP;Barbie, DA;Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor SpheroidsCancer discovery29101162Freshly isolated human CD4+ and CD8+ T cells were obtained from AllCells. Cells were spun down and resuspended in serum free X-vivo15 media (Lonza Walkersville, Inc.) supplemented with 5 ng/mL IL17 and incubated overnight at 37°C. Cells were plated oFull Paper
2018Ryu, JK;Rafalski, VA;Meyer-Franke, A;Adams, RA;Poda, SB;Rios Coronado, PE;Pedersen, LØ;Menon, V;Baeten, KM;Sikorski, SL;Bedard, C;Hanspers, K;Bardehle, S;Mendiola, AS;Davalos, D;Machado, MR;Chan, JP;Plastira, I;Petersen, MA;Pfaff, SJ;Ang, KK;Hallenbeck, KK;Syme, C;Hakozaki, H;Ellisman, MH;Swanson, RA;Zamvil, SS;Arkin, MR;Zorn, SH;Pico, AR;Mucke, L;Freedman, SB;Stavenhagen, JB;Nelson, RB;Akassoglou, K;Fibrin-targeting immunotherapy protects against neuroinflammation and neurodegenerationNature immunology30323343wed by an incubation of 6.88 μM CD11b I-domain for 2 h at 25 ˚C. Cell Culture. Primary microglia and BMDMs were isolated, cultured, and treated with fibrin or LPS as previously described16. PBMCs (AllCells) (2×106 cells/mL) were plated in RPMI-1640Full Paper
2018Chorzalska, A;Morgan, J;Ahsan, N;Treaba, DO;Olszewski, AJ;Petersen, M;Kingston, N;Cheng, Y;Lombardo, K;Schorl, C;Yu, X;Zini, R;Pacilli, A;Tepper, A;Coburn, J;Hryniewicz-Jankowska, A;Zhao, TC;Oancea, E;Reagan, JL;Liang, O;Kotula, L;Quesenberry, PJ;Gruppuso, PA;Manfredini, R;Vannucchi, AM;Dubielecka, PM;Bone marrow-specific loss of ABI1 induces myeloproliferative neoplasm with features resembling human myelofibrosisBlood30213875CD34 + cells were isolated from the bone marrow of patients with PMF or from healthy marrow purchased from AllCells (Alameda, CA). Granulocytes were isolated from peripheral SaveFull Paper
2018Cairo, M;Parsons, S;Braniecki, S;Talano, J;Moore, T;Shi, Q;Keever-Taylor, C;Weinberg, R;Grossman, B;Dozor, A;Friedman, D;McKinstry, R;Verbsky, J;Moorthy, C;Weidner, R;Rodday, A;Ayello, J;Semidei-Pomales, M;Flower, A;Chu, Y;Morris, E;Mahanti, H;Fabricatore, S;Militano, O;Klejmont, L;van de Ven, C;Baxter-Lowe, L;Vichinsky, E;Walters, M;Shenoy, S;Significantly Improved Long Term Health Related Quality of Life (HRQL) and Neurocognition Following Familial Haploidentical Stem Cell Transplantation (HISCT) Utilizing CD34 Enrichment and Mononuclear (CD3) Addback in High Risk Patients with Sickle Cell Disease (SCD)BloodViaCord Processing Lab: Other: Medical Director; AllCells Inc.: Other: Medical Director. Shenoy: Novartis, Vertex, Bluebird Bio: Honoraria, Membership on an entity's Board of Directors or advisory committeesFull Paper
2018Jamieson, CHM;Millan, MT;Creasey, AA;Lomax, G;Donohoe, ME;Walters, MC;Abedi, M;Bota, DA;Zaia, JA;Adams, JS;CIRM Alpha Stem Cell Clinics: Collaboratively Addressing Regenerative Medicine ChallengesCell stem cell29859170Declaration of Interests All authors have CIRM funding to declare. M.C.W. is a Consultant for Bioverativ, Inc., Bluebird Bio, Inc., and Sangamo Biotherapeutics and a Medical Director for AllCells, Inc. and ViaCord Processing LabFull Paper
2018Cesana, M;Guo, MH;Cacchiarelli, D;Wahlster, L;Barragan, J;Doulatov, S;Vo, LT;Salvatori, B;Trapnell, C;Clement, K;Cahan, P;Tsanov, KM;Sousa, PM;Tazon-Vega, B;Bolondi, A;Giorgi, FM;Califano, A;Rinn, JL;Meissner, A;Hirschhorn, JN;Daley, GQ;A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout DevelopmentCell stem cell29625070Human CD34 + cells (Lonza and AllCells) from all sources were obtained as viable frozen states. The ages of the individuals used for the profiling and treatments are as follows: FL- SaveFull Paper
2018Kao, YR;Chen, J;Narayanagari, SR;Todorova, TI;Aivalioti, MM;Ferreira, M;Ramos, PM;Pallaud, C;Mantzaris, I;Shastri, A;Bussel, JB;Verma, A;Steidl, U;Will, B;Thrombopoietin receptor-independent stimulation of hematopoietic stem cells by eltrombopagScience translational medicine30209246Healthy cryopreserved human bone marrow cells were purchased from AllCells and ReachBio Research Labs; fresh unprocessed bone marrow cells were from Lonza. Samples of SaveFull Paper
2018Kou, X;Xu, X;Chen, C;Sanmillan, ML;Cai, T;Zhou, Y;Giraudo, C;Le, A;Shi, S;The Fas/Fap-1/Cav-1 complex regulates IL-1RA secretion in mesenchymal stem cells to accelerate wound healingScience translational medicine29540618Human bone marrow aspirates from healthy human adult volunteers (20 to 35 years of age) were purchased from AllCells LLC. Human GMSCs and SMSCs were isolated from the SaveFull Paper
2018Lai, D;Chen, M;Su, J;Liu, X;Rothe, K;Hu, K;Forrest, DL;Eaves, CJ;Morin, GB;Jiang, X;PP2A inhibition sensitizes cancer stem cells to ABL tyrosine kinase inhibitors in BCR-ABL+ human leukemiaScience translational medicine29437150Overcoming drug resistance and targeting leukemic stem cells (LSCs) remain major challenges in curing BCR-ABL + human leukemia. Using an advanced drug/proliferation screen, we SaveFull Paper
2018Ryan, DE;Taussig, D;Steinfeld, I;Phadnis, SM;Lunstad, BD;Singh, M;Vuong, X;Okochi, KD;McCaffrey, R;Olesiak, M;Roy, S;Yung, CW;Curry, B;Sampson, JR;Bruhn, L;Dellinger, DJ;Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAsNucleic acids research29216382Nucleofector kit (V4SP-3096). Cells were harvested at 48 hr post-transfection. Mobilized human peripheral blood CD34-positive hematopoietic stem and progenitor cells (CD34+ HSPCs) were purchased from AllCells (Alameda, CA) and thawed per manufacturerFull Paper
2018Syed, SA;Beurel, E;Loewenstein, DA;Lowell, JA;Craighead, WE;Dunlop, BW;Mayberg, HS;Dhabhar, F;Dietrich, WD;Keane, RW;de Rivero Vaccari, JP;Nemeroff, CB;Defective Inflammatory Pathways in Never-Treated Depressed Patients Are Associated with Poor Treatment ResponseNeuron30146307PBMCs from a non-hispanic white female healthy donor (age 50, weight: 142 lbs, height 68 in) unmedicated for a week, were freshly isolated at ALLCELLS. No other information SaveFull Paper
2018Lei, L;Chen, H;Xue, W;Yang, B;Hu, B;Wei, J;Wang, L;Cui, Y;Li, W;Wang, J;Yan, L;Shang, W;Gao, J;Sha, J;Zhuang, M;Huang, X;Shen, B;Yang, L;Chen, J;APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaksNature structural & molecular biology29323274Cryopreserved human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells, LLC (PB003F). CD8+ T cells were pre-enriched from the PBMCs with Easysep Human CD8+ T cell enrichment kit (19053, Stemcell Technologies) according to the manFull Paper
2018Stoeckius, M;Zheng, S;Houck-Loomis, B;Hao, S;Yeung, BZ;Mauck, WM;Smibert, P;Satija, R;Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomicsGenome biology30567574urther enable multiplexing strategies to generalize to diverse experiments regardless of species, tissue, or technology. Methods PBMC genotyping Peripheral blood mononuclear cells were obtained from AllCells (USA). Genomic DNA was purified using theFull Paper
2018Xu, Y;Zhao, W;Olson, SD;Prabhakara, KS;Zhou, X;Alternative splicing links histone modifications to stem cell fate decisionGenome biology30217220After reaching ~ 70% confluence, the adherent cells were harvested at the passage 1 (P1) hAT-MSC/AEY239. hBM-MSCs were isolated from commercially available fresh human bone marrow (hBM) aspirates (AllCells, Emeryville, CA, USA) and expanded followingFull Paper
2018Salas, LA;Koestler, DC;Butler, RA;Hansen, HM;Wiencke, JK;Kelsey, KT;Christensen, BC;An optimized library for reference-based deconvolution of whole-blood biospecimens assayed using the Illumina HumanMethylationEPIC BeadArrayGenome biology29843789urity cell subtypes (neutrophils (Neu), monocytes (Mono), B lymphocytes (Bcells), T helper lymphocytes (CD4T), T cytotoxic lymphocytes (CD8T), and natural killer lymphocytes (NK)) were purchased from AllCells corporation (Alameda, CA, USA) and STEMCFull Paper
2018Lim, SH;Kathuria, H;Tan, JJY;Kang, L;3D printed drug delivery and testing systems - a passing fad or the future?Advanced drug delivery reviews29778901Primary human dental pulp stem cells (DPSC) from a single donor (DPF003, AllCells, USA) were cultured in a complete growth media containing Dulbecco's Modified Eagle's Medium, supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. CFull Paper
2018Marabissi, D;Bartoli, G;Micciullo, L;Fantacci, R;Energy efficient multicast beamforming in ultra dense networks2018 IEEE Wireless Communications and Networking Conference (WCNC)This figure clearly shows the better behavior of our method in comparison with static approaches (ie, OnlyMacro, AllCells). Moreover, we can see that even if sub-optimum, our method is SaveFull Paper
2018Cao, L;Pauthner, M;Andrabi, R;Rantalainen, K;Berndsen, Z;Diedrich, JK;Menis, S;Sok, D;Bastidas, R;Park, SR;Delahunty, CM;He, L;Guenaga, J;Wyatt, RT;Schief, WR;Ward, AB;Yates, JR;Burton, DR;Paulson, JC;Differential processing of HIV envelope glycans on the virus and soluble recombinant trimerNature communications30209313Primary PBMC samples were obtained through the commercial vendor AllCells (Emeryville, CA The leukapak sample was obtained from a commercial vendor (AllCells) using a protocol SaveFull Paper
2018Mezger, A;Klemm, S;Mann, I;Brower, K;Mir, A;Bostick, M;Farmer, A;Fordyce, P;Linnarsson, S;Greenleaf, W;High-throughput chromatin accessibility profiling at single-cell resolutionNature communications30194434, and B cells were isolated from whole blood (AllCells, CA, USA) using EasySep Direct Human All human cells were obtained from AllCells with explicit consent to publish data for broad SaveFull Paper
2018Lyu, G;Guan, Y;Zhang, C;Zong, L;Sun, L;Huang, X;Huang, L;Zhang, L;Tian, XL;Zhou, Z;Tao, W;TGF-β signaling alters H4K20me3 status via miR-29 and contributes to cellular senescence and cardiac agingNature communications29967491The culture medium was MEM supplemented with 10% FBS (GIBCO), and the culture dish was gelatin-coated before inoculation of primary human umbilical vascular endothelial cells (HUVECs) purchased from ALLCELLS Co. Ltd., Shanghai, China. Cells were testFull Paper
2018Xu, L;Gordon, R;Farmer, R;Pattanayak, A;Binkowski, A;Huang, X;Avram, M;Krishna, S;Voll, E;Pavese, J;Chavez, J;Bruce, J;Mazar, A;Nibbs, A;Anderson, W;Li, L;Jovanovic, B;Pruell, S;Valsecchi, M;Francia, G;Betori, R;Scheidt, K;Bergan, R;Precision therapeutic targeting of human cancer cell motilityNature communications29934502od cells, hemoglobin, and platelets. Hematopoietic stem cell colony formation assay Fourteen-day colony formation assays were conducted as described by us39, using human cord blood CD34+ stem cells (AllCells Inc.), using MethoCultExpress colony groFull Paper
2018Yang, R;Yu, T;Kou, X;Gao, X;Chen, C;Liu, D;Zhou, Y;Shi, S;Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoterNature communications29858571(PFA, Sigma-Aldrich) after 16 days of culturing. Clusters with more than 50 cells were counted as colonies under microscopy. Isolation and culture of human BMMSCs We purchased human bone marrow from AllCells LLC (Alameda, CA, USA), which were aspiraFull Paper
2018Lummertz da Rocha, E;Rowe, RG;Lundin, V;Malleshaiah, M;Jha, DK;Rambo, CR;Li, H;North, TE;Collins, JJ;Daley, GQ;Reconstruction of complex single-cell trajectories using CellRouterNature communications29497036es) would act as perturbations that could be explored by CLR to identify putative regulatory relationships. In vitro erythroid differentiation Mobilized peripheral blood CD34+ stem/progenitor cells (AllCells) were obtained from three independent donFull Paper
2018Ravi, R;Noonan, KA;Pham, V;Bedi, R;Zhavoronkov, A;Ozerov, IV;Makarev, E;V Artemov, A;Wysocki, PT;Mehra, R;Nimmagadda, S;Marchionni, L;Sidransky, D;Borrello, IM;Izumchenko, E;Bedi, A;Bifunctional immune checkpoint-targeted antibody-ligand traps that simultaneously disable TGFβ enhance the efficacy of cancer immunotherapyNature communications29467463)(4-8-week old) were irradiated at 200 cGy, followed by adoptive transfer of human BM CD34 + cells (7 × 10 4 per mouse) from a normal donor (HLA-matched to the tumor) (ALLCELLS) SaveFull Paper
2018Bak, RO;Dever, DP;Porteus, MH;CRISPR/Cas9 genome editing in human hematopoietic stem cellsNature protocols29370156Fresh or frozen CD34 + human HSPCs (either acquired per institutional protocol or purchased from various commercial sources, including AllCells (CB-derived, cat. no. CB008F; mPB- SaveFull Paper
2018Adine, C;Ng, KK;Rungarunlert, S;Souza, GR;Ferreira, JN;Engineering innervated secretory epithelial organoids by magnetic three-dimensional bioprinting for stimulating epithelial growth in salivary glandsBiomaterials30025245Human dental pulp stem cells (hDPSCs) were commercially obtained from AllCells (Alameda, CA, USA) from three different donors. These stem cells were derived from the pulp tissues of exfoliated deciduous teeth, primary incisors, or permanent third molFull Paper
2018Jiang, L;Zhang, W;Wei, L;Zhou, Q;Yang, G;Qian, N;Tang, Y;Gao, Y;Jiang, X;Early effects of parathyroid hormone on vascularized bone regeneration and implant osseointegration in aged ratsBiomaterials29960821Human umbilical vein endothelial cells (HUVECs) (Allcells, Shanghai, China) were cultured and their in vitro recruitment and angiogenesis abilities were measured. PTH (1-34) was added at a concentration of 10 nM in the lower chambers of 24-well transFull Paper
2018Odegaard, JI;Vincent, JJ;Mortimer, S;Vowles, JV;Ulrich, BC;Banks, KC;Fairclough, SR;Zill, OA;Sikora, M;Mokhtari, R;Abdueva, D;Nagy, RJ;Lee, CE;Kiedrowski, LA;Paweletz, CP;Eltoukhy, H;Lanman, RB;Chudova, DI;Talasaz, A;Validation of a Plasma-Based Comprehensive Cancer Genotyping Assay Utilizing Orthogonal Tissue- and Plasma-Based MethodologiesClinical cancer research : an official journal of the American Association for Cancer Research29691297Contrived analytic samples were generated using similarly prepared cfDNA from healthy donors (AllCells, Inc.) and cfDNA isolated as above from the culture supernatant of model cell SaveFull Paper
2018L'Italien, L;Orozco, O;Abrams, T;Cantagallo, L;Connor, A;Desai, J;Ebersbach, H;Gelderblom, H;Hoffmaster, K;Lees, E;Maacke, H;Schleyer, S;Skegro, D;Lee-Hoeflich, ST;Mechanistic Insights of an Immunological Adverse Event Induced by an Anti-KIT Antibody Drug Conjugate and Mitigation StrategiesClinical cancer research : an official journal of the American Association for Cancer Research29615457Human peripheral blood cell-derived mast cells (PBC-MCs) were generated from mobilized peripheral blood CD34 + cells isolated from healthy donors (AllCells #mPB017F). Mast cells SaveFull Paper
2018Cromer, MK;Vaidyanathan, S;Ryan, DE;Curry, B;Lucas, AB;Camarena, J;Kaushik, M;Hay, SR;Martin, RM;Steinfeld, I;Bak, RO;Dever, DP;Hendel, A;Bruhn, L;Porteus, MH;Global Transcriptional Response to CRISPR/Cas9-AAV6-Based Genome Editing in CD34+ Hematopoietic Stem and Progenitor CellsMolecular therapy : the journal of the American Society of Gene Therapy30005866CD34+ HSPCs used for gene expression microarrays were obtained as frozen stock derived from mobilized peripheral blood thawed according to manufacturer’s instructions (AllCells). For follow-up experiments, CD34+ HSPCs were obtained fresh from cord blFull Paper
2018Ligtenberg, MA;Pico de Coaña, Y;Shmushkovich, T;Yoshimoto, Y;Truxova, I;Yang, Y;Betancur-Boissel, M;Eliseev, AV;Wolfson, AD;Kiessling, R;Self-Delivering RNAi Targeting PD-1 Improves Tumor-Specific T Cell Functionality for Adoptive Cell Therapy of Malignant MelanomaMolecular therapy : the journal of the American Society of Gene Therapy29735366ation (Ficoll-Paque Plus; GE Healthcare). T cells were purified using the Pan T cell isolation kit (Miltenyi Biotec) following manufacturer’s instructions. Peripheral blood CD3+ Pan T cells from AllCells (PB009-1F) were used for sdRNA validation andFull Paper
2018Georgiadis, C;Preece, R;Nickolay, L;Etuk, A;Petrova, A;Ladon, D;Danyi, A;Humphryes-Kirilov, N;Ajetunmobi, A;Kim, D;Kim, JS;Qasim, W;Long Terminal Repeat CRISPR-CAR-Coupled "Universal" T Cells Mediate Potent Anti-leukemic EffectsMolecular therapy : the journal of the American Society of Gene Therapy29605708CliniMACS Prodigy using tubing set TS520 (130-019-002, Miltenyi Biotec) and used cryopreserved leukapheresis harvests (Allcells, Alameda, USA) cultured in TexMACS good manufacturing practice (GMP) medium (170-076-307, Miltenyi Biotec) supplemented wiFull Paper
2018Heffner, GC;Bonner, M;Christiansen, L;Pierciey, FJ;Campbell, D;Smurnyy, Y;Zhang, W;Hamel, A;Shaw, S;Lewis, G;Goss, KA;Garijo, O;Torbett, BE;Horton, H;Finer, MH;Gregory, PD;Veres, G;Prostaglandin E2 Increases Lentiviral Vector Transduction Efficiency of Adult Human Hematopoietic Stem and Progenitor CellsMolecular therapy : the journal of the American Society of Gene Therapy29102562CD34-enriched mPB samples from healthy human donors were obtained from AllCells (Emeryville, CA, USA), Key Biologics (Memphis, TN, USA), and primary patients under Save;Methods Cell Culture Research-grade GFP-, globin-, and ALDP-containing LVVs werFull Paper
2018Mokhtari, S;Colletti, E;Yin, W;Sanada, C;Lamar, Z;Simmons, PJ;Walker, S;Bishop, C;Atala, A;Zanjani, ED;Porada, CD;Almeida-Porada, G;A human bone marrow mesodermal-derived cell population with hemogenic potentialLeukemia29467489al bone tissues were obtained from Advanced Bioscience Resources (Alameda, CA). Human adult bone marrow (BM) was obtained from healthy adult donors after informed consent, or from commercial sources (AllCells, LLC, Alameda, CA). Fetal and adult tissuFull Paper
2018Tan, YT;Ye, L;Xie, F;Beyer, AI;Muench, MO;Wang, J;Chen, Z;Liu, H;Chen, SJ;Kan, YW;Respecifying human iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells with a single factorProceedings of the National Academy of Sciences of the United States of America29386396Frozen human PB mobilized CD34+ cells and PB mononuclear cells were purchased from AllCells (www.allcells.com). The protocols involving human iPSCs were approved by Human Research Committee and Human Gamete, Embryo and Stem Cells Research Committee (Full Paper
2018Li, Y;Carpenito, C;Wang, G;Surguladze, D;Forest, A;Malabunga, M;Murphy, M;Zhang, Y;Sonyi, A;Chin, D;Burtrum, D;Inigo, I;Pennello, A;Shen, L;Malherbe, L;Chen, X;Hall, G;Haidar, JN;Ludwig, DL;Novosiadly, RD;Kalos, M;Discovery and preclinical characterization of the antagonist anti-PD-L1 monoclonal antibody LY3300054Journal for immunotherapy of cancer29712568s analyzed using GraphPad Prism software [25]. Mixed leukocyte reaction (MLR) CD14+ monocytes were isolated from frozen human peripheral blood mononuclear cells (PBMC) obtained from a healthy donor (AllCells, Alameda, CA) with Human Monocyte IsolatiFull Paper
2018Liu, Y;Jing, H;Kou, X;Chen, C;Liu, D;Jin, Y;Lu, L;Shi, S;PD-1 is required to maintain stem cell properties in human dental pulp stem cellsCell death and differentiation29472716Isolation and culture of human BMMSCs Human bone marrow aspirates from healthy human adult volunteers (20-35 years of age) were purchased from AllCells LLC. hBMMSCs were cultured as reported previously [40]Full Paper
2018Willingham, SB;Ho, PY;Hotson, A;Hill, C;Piccione, EC;Hsieh, J;Liu, L;Buggy, JJ;McCaffery, I;Miller, RA;A2AR Antagonism with CPI-444 Induces Antitumor Responses and Augments Efficacy to Anti-PD-(L)1 and Anti-CTLA-4 in Preclinical ModelsCancer immunology research30131376PBMCs were purchased from AllCells and either used fresh or cryopreserved for future use. Fresh PBMCs were cultured at a density of 10 7 cells/mL, and cryopreserved cells were SaveFull Paper
2018Siriwon, N;Kim, YJ;Siegler, E;Chen, X;Rohrs, JA;Liu, Y;Wang, P;CAR-T Cells Surface-Engineered with Drug-Encapsulated Nanoparticles Can Ameliorate Intratumoral T-cell HypofunctionCancer immunology research29720380Frozen human peripheral blood mononuclear cells (PBMC) were obtained from AllCells. Thawed PBMCs were cultured in T-cell medium (TCM) containing X-VIVO 15 serum-free SaveFull Paper
2018Oeller, M;Laner-Plamberger, S;Hochmann, S;Ketterl, N;Feichtner, M;Brachtl, G;Hochreiter, A;Scharler, C;Bieler, L;Romanelli, P;Couillard-Despres, S;Russe, E;Schallmoser, K;Strunk, D;Selection of Tissue Factor-Deficient Cell Transplants as a Novel Strategy for Improving Hemocompatibility of Human Bone Marrow Stromal CellsTheranostics29507631Three additional BM samples (10 mL each) were purchased via CellSystems from ALLCELLS (USA). The mean age of the male BM donors was 34 years (range 20-46) and of the SaveFull Paper
2018de la Puente, P;Luderer, MJ;Federico, C;Jin, A;Gilson, RC;Egbulefu, C;Alhallak, K;Shah, S;Muz, B;Sun, J;King, J;Kohnen, D;Salama, NN;Achilefu, S;Vij, R;Azab, AK;Enhancing proteasome-inhibitory activity and specificity of bortezomib by CD38 targeted nanoparticles in multiple myelomaJournal of controlled release : official journal of the Controlled Release Society29196043Bone Marrow mononuclear cells (BM MNCs) from 5 different patient samples were purchased from Allcells (Alameda, CA). Peripheral blood mononuclear cells (PB MNCs) were SaveFull Paper
2018Chen, Y;Mao, P;Snijders, AM;Wang, D;Senescence chips for ultrahigh-throughput isolation and removal of senescent cellsAging cell29336105Fresh human whole blood from healthy donors collected within 24 hr was purchased from AllCells Inc. (Alameda, CA). Human MSCs were purchased from Lonza (Lonza, Swiss). SaveFull Paper
2018Charlesworth, CT;Camarena, J;Cromer, MK;Vaidyanathan, S;Bak, RO;Carte, JM;Potter, J;Dever, DP;Porteus, MH;Priming Human Repopulating Hematopoietic Stem and Progenitor Cells for Cas9/sgRNA Gene TargetingMolecular therapy. Nucleic acids30195800CD34 + HSPCs derived from mobilized peripheral blood were purchased from AllCells, and CD34 + HSPCs derived from cord blood were provided from donors under informed consent Save;ollowing the manufacturer’s instructions. Titers were measured after bFull Paper
2018Vaidyanathan, S;Azizian, KT;Haque, AKMA;Henderson, JM;Hendel, A;Shore, S;Antony, JS;Hogrefe, RI;Kormann, MSD;Porteus, MH;McCaffrey, AP;Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC PurificationMolecular therapy. Nucleic acids30195789CD34 + HSPCs derived from mobilized peripheral blood donated by male donors were purchased from AllCells (Alemeda, CA). Cells were thawed according to the manufacturer's Save;tection System luminometer (Promega, E8032) with a 10-s integration per wFull Paper
2018Yoshinaga, T;Seki, T;Miki, S;Miyamoto, T;Suyama-Kagitani, A;Kawauchi-Miki, S;Kobayashi, M;Sato, A;Stewart, E;Underwood, M;Fujiwara, T;Novel secondary mutations C56S and G149A confer resistance to HIV-1 integrase strand transfer inhibitorsAntiviral research29410019kanamycin sulfate. Peripheral blood mononuclear cells (PBMCs; AllCells, Alameda, CA) were cultured in RPMI 1640 medium supplemented with 10% FBS, 60 μg/mL of kanamycin sulfate, and 10 μg/mL of phytohemagglutinin (PHA)-M (Roche, Indianapolis, IN) forFull Paper
2018Li, S;Hong, Z;Wang, Z;Li, F;Mei, J;Huang, L;Lou, X;Zhao, S;Song, L;Chen, W;Wang, Q;Liu, H;Cai, Y;Yu, H;Xu, H;Zeng, G;Wang, Q;Zhu, J;Liu, X;Tan, N;Wang, C;The Cyclopeptide Astin C Specifically Inhibits the Innate Immune CDN Sensor STINGCell reports30566866hPBMCs and IMR-90 (female) cells were purchased from AllCells and Cobioer Bioscience, respectively. The gender information of hPBMCs is not available from the supplier. The SaveFull Paper
2018Osorio, FG;Rosendahl Huber, A;Oka, R;Verheul, M;Patel, SH;Hasaart, K;de la Fonteijne, L;Varela, I;Camargo, FD;van Boxtel, R;Somatic Mutations Reveal Lineage Relationships and Age-Related Mutagenesis in Human HematopoiesisCell reports30485801nd Cat# 302005; RRID:AB_314205 CD11c-FITC BioLegend Cat# 301603; RRID:AB_314173 ------------------------- Biological Samples ------------------------- Whole bone marrow samples A,C,D,E AllCells ABM001 Whole bone marrow sample B BFull Paper
2018Zhu, YP;Padgett, L;Dinh, HQ;Marcovecchio, P;Blatchley, A;Wu, R;Ehinger, E;Kim, C;Mikulski, Z;Seumois, G;Madrigal, A;Vijayanand, P;Hedrick, CC;Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone MarrowCell reports30157427Fresh BM samples of anonymous healthy adult donors were obtained from AllCells, Inc. (Alameda, CA). The cells were stained for either flow cytometry or FACS-sorting following protocols described in the Flow Cytometry and Cell Sorting sectionFull Paper
2018Schaer, DA;Beckmann, RP;Dempsey, JA;Huber, L;Forest, A;Amaladas, N;Li, Y;Wang, YC;Rasmussen, ER;Chin, D;Capen, A;Carpenito, C;Staschke, KA;Chung, LA;Litchfield, LM;Merzoug, FF;Gong, X;Iversen, PW;Buchanan, S;de Dios, A;Novosiadly, RD;Kalos, M;The CDK4/6 Inhibitor Abemaciclib Induces a T Cell Inflamed Tumor Microenvironment and Enhances the Efficacy of PD-L1 Checkpoint BlockadeCell reports29539425Breast cancer cell lines MCF-7 and MDA-MB-468 (American Type Culture Collection [ATCC]) were treated with DMSO and abemaciclib (500 nM) for 8 days. RNA was isolated as described above. Jurkat T cells (ATCC) or primary human T cells (AllCells) were stFull Paper
2018Shi, J;Zhang, M;Zhang, L;Deng, H;Epigallocatechin-3-gallate attenuates microcystin-LR-induced apoptosis in human umbilical vein endothelial cells through activation of the NRF2/HO-1 pathwayEnvironmental pollution (Barking, Essex : 1987)29679944HUVECs were obtained from Allcells Technology (Shanghai, China) and seeded in complete medium (HUVEC-004, AllCells Technology) in a humidified atmosphere of 5% CO 2 at 37 SaveFull Paper
2018Wienert, B;Shin, J;Zelin, E;Pestal, K;Corn, JE;In vitro-transcribed guide RNAs trigger an innate immune response via the RIG-I pathwayPLoS biology30011268HSPCs from mobilized peripheral blood (Allcells) were thawed and cultured in StemSpan SFEM medium (StemCell Technologies) supplemented with StemSpan CC110 cocktail ( SaveFull Paper
2018Yang, W;Sun, Z;Hua, C;Wang, Q;Xu, W;Deng, Q;Pan, Y;Lu, L;Jiang, S;Chidamide, a histone deacetylase inhibitor-based anticancer drug, effectively reactivates latent HIV-1 provirusMicrobes and infection29126877J-Lat A2 cells [25] (obtained from NIH AIDS Reagent Program), J-Lat C11 cells [26], PBMCs (Allcells), ACH-2 cells [27] and U1 cells [28] were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS) and 1% Pen/strep in a 37 °C incubator containFull Paper
2018Ito, R;Maruoka, S;Soda, K;Katano, I;Kawai, K;Yagoto, M;Hanazawa, A;Takahashi, T;Ogura, T;Goto, M;Takahashi, R;Toyoshima, S;Okayama, Y;Izuhara, K;Gon, Y;Hashimoto, S;Ito, M;Nunomura, S;A humanized mouse model to study asthmatic airway inflammation via the human IL-33/IL-13 axisJCI insight30385714Commercially available human cord blood-derived CD34 + cells (AllCells LLC or Lonza) were used in this study. The cells were prepared according to the manufacturer's instructions. SaveFull Paper
2018Antebi, B;Rodriguez, LA;Walker, KP;Asher, AM;Kamucheka, RM;Alvarado, L;Mohammadipoor, A;Cancio, LC;Short-term physiological hypoxia potentiates the therapeutic function of mesenchymal stem cellsStem cell research & therapy30305185arrow MSCs (pMSCs) were isolated from mononuclear cells (MNCs) obtained from bone marrow aspirates, as previously described [39]. Human bone marrow MSCs (hMSCs) were isolated from MNCs purchased from AllCells LLC (Emeryville, CA, USA). MNCs were cultFull Paper
2018Senyuk, V;Patel, P;Mahmud, N;Rondelli, D;Blockade of TNFα to Improve Human CD34+ Cell Repopulating Activity in Allogeneic Stem Cell TransplantationFrontiers in immunology30723481Healthy donor G-CSF mobilized peripheral blood stem cells (PBSC) from AllCells (Alameda, CA) and PB cells from healthy volunteers were utilized in this study. Mononuclear cells ( SaveFull Paper
2018Cui, C;Su, M;Lin, Y;Lai, L;A CD300c-Fc Fusion Protein Inhibits T Cell ImmunityFrontiers in immunology30498497peripheral blood CD3 + Pan T Cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan-T labeling system were purchased from ALLCELLS, SaveFull Paper
2018Cobbold, SP;Adams, E;Howie, D;Waldmann, H;CD4+ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium DevelopmentFrontiers in immunology29967616t CV (%) is indicated. In all experiments, P values ofFull Paper
2018Burton, OT;Epp, A;Fanny, ME;Miller, SJ;Stranks, AJ;Teague, JE;Clark, RA;van de Rijn, M;Oettgen, HC;Tissue-Specific Expression of the Low-Affinity IgG Receptor, FcγRIIb, on Human Mast CellsFrontiers in immunology29928276genic for membrane-bound human stem cell factor (SCF) [NOD.Cg-PrkdcscidIl2rgtm1Wjl Tg(PGK1-KITLG*220)441Daw/SzJ] were engrafted with 5 × 104 CD34+ hematopoietic stem cells (HSC) from cord blood (AllCells) for 16-24 weeks. Wild-type BALB/c, C57BL/6J,Full Paper
2018White, CH;Beliakova-Bethell, N;Lada, SM;Breen, MS;Hurst, TP;Spina, CA;Richman, DD;Frater, J;Magiorkinis, G;Woelk, CH;Transcriptional Modulation of Human Endogenous Retroviruses in Primary CD4+ T Cells Following Vorinostat TreatmentFrontiers in immunology29706951rials and Methods Isolation of Primary CD4+ T Cells For subsequent total RNA-Seq analysis, cryopreserved primary CD4+ T cells that were viably frozen were obtained from four different healthy donors (AllCells, Inc., Emeryville, CA, USA) and thawed inFull Paper
2018Bhaskaran, N;Liu, Z;Saravanamuthu, SS;Yan, C;Hu, Y;Dong, L;Zelenka, P;Zheng, L;Bletsos, V;Harris, R;Harrington, B;Weinberg, A;Thiele, CJ;Ye, F;Pandiyan, P;Identification of Casz1 as a Regulatory Protein Controlling T Helper Cell Differentiation, Inflammation, and ImmunityFrontiers in immunology29467767done at NIAID, NIH under an approved protocol, and in compliance with the NIAID Institutional Animal Care and Use Committee’s guidelines. Human cells were obtained from commercially available PBMC (AllCells). Reagents and Antibodies Purified or fluoFull Paper
2018Hanazawa, A;Ito, R;Katano, I;Kawai, K;Goto, M;Suemizu, H;Kawakami, Y;Ito, M;Takahashi, T;Generation of Human Immunosuppressive Myeloid Cell Populations in Human Interleukin-6 Transgenic NOG MiceFrontiers in immunology29456539ined in the Central Institute for Experimental Animals (CIEA) under specific pathogen-free conditions. Transplantation of Human HSCs Human umbilical-cord-blood-derived CD34+ HSCs were purchased from Allcells (Alameda, CA, USA). For transplantation,Full Paper
2018Seto, AG;Beatty, X;Lynch, JM;Hermreck, M;Tetzlaff, M;Duvic, M;Jackson, AL;Cobomarsen, an oligonucleotide inhibitor of miR-155, co-ordinately regulates multiple survival pathways to reduce cellular proliferation and survival in cutaneous T-cell lymphomaBritish journal of haematology30125933miR‐155, a microRNA associated with poor prognosis in lymphoma and leukaemia, has been implicated in the progression of mycosis fungoides ( MF ), the most common form of SaveFull Paper
2018Makar, T;Nimmagadda, VK;Guda, PR;Hampton, B;Huang, W;Kane, MA;Fishman, PS;Pessac, B;Bever, CT;Trisler, D;A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditionsStem cell research & therapy29720239Human buffy coat cells and CD34 + cells from peripheral blood were purchased from ALLCELLS (Alameda, CA, USA). Human buffy coat cells were obtained by differential SaveFull Paper
2018Fujisawa, K;Hara, K;Takami, T;Okada, S;Matsumoto, T;Yamamoto, N;Sakaida, I;Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cellsStem cell research & therapy29625581MSCs were derived from bone marrow cells (AllCells, Alameda, CA,USA) and were used at passage (P)4. Mononuclear cells (MNCs) were purchased from Lonza, Tokyo, Japan. Flow SaveFull Paper
2018Thekkeparambil Chandrabose, S;Sriram, S;Subramanian, S;Cheng, S;Ong, WK;Rozen, S;Kasim, NHA;Sugii, S;Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogrammingStem cell research & therapy29559008The list of commercial (Lonza and Allcells) and patient-derived dental cells used in this manuscript can be found in Additional file 1: Table S1. DPSCs were grown in vitro in Poietics SaveFull Paper
2018Johnson, HWB;Lowe, E;Anderl, JL;Fan, A;Muchamuel, T;Bowers, S;Moebius, DC;Kirk, C;McMinn, DL;Required Immunoproteasome Subunit Inhibition Profile for Anti-Inflammatory Efficacy and Clinical Candidate KZR-616 ((2 S,3 R)- N-(( S)-3-(Cyclopent-1-en-1-yl)-1-(( R)-2-methyloxiran-2-yl)-1-oxopropan-2-yl)-3-hydroxy-3-(4-methoxyphenyl)-2-(( S)-2-(2-morpholinoacetamido)propanamido)propenamide)Journal of medicinal chemistry30380863Cryopreserved peripheral blood mononuclear cells (PBMCs) from normal healthy volunteers were purchased from AllCells (Alameda, CA) and cultured in RPMI 1640 media (with 300 mg/L l-glutamine) supplemented with 5% heat-inactivated FBS, 100 IU penicilliFull Paper
2018Vazquez, ML;Kaila, N;Strohbach, JW;Trzupek, JD;Brown, MF;Flanagan, ME;Mitton-Fry, MJ;Johnson, TA;TenBrink, RE;Arnold, EP;Basak, A;Heasley, SE;Kwon, S;Langille, J;Parikh, MD;Griffin, SH;Casavant, JM;Duclos, BA;Fenwick, AE;Harris, TM;Han, S;Caspers, N;Dowty, ME;Yang, X;Banker, ME;Hegen, M;Symanowicz, PT;Li, L;Wang, L;Lin, TH;Jussif, J;Clark, JD;Telliez, JB;Robinson, RP;Unwalla, R;Identification of N-{cis-3-[Methyl(7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino]cyclobutyl}propane-1-sulfonamide (PF-04965842): A Selective JAK1 Clinical Candidate for the Treatment of Autoimmune DiseasesJournal of medicinal chemistry29298069Cryopreserved human bone marrow CD34+ cells (cat. no. ABM022F) were purchased from Allcells. Frozen bone marrow CD34+ cells were thawed, washed once with IMEM, resuspended in Stem Span medium (cat. no. 09600; STEMCELL Technologies, Vancouver, Canada)Full Paper
2018Xu, H;Liao, C;Zuo, P;Liu, Z;Ye, BC;Magnetic-Based Microfluidic Device for On-Chip Isolation and Detection of Tumor-Derived ExosomesAnalytical chemistry30234974CD63 antibody (anti-CD63) from Biolegend (USA), biotin-labeled mouse antihuman CD9 antibody (anti-CD9) from Allcells Biotech Co., Ltd. (Shanghai, China), and HRP-conjugated antimouse IgG from QF Biosciences Co., Ltd. (Shanghai, China). MagCapture ExoFull Paper
2018Xu, H;Liao, C;Liu, Y;Ye, BC;Liu, B;Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living CellsAnalytical chemistry29508999Human umbilical vein endothelial cells (HUVECs) (ALLCELLS, Shanghai) were cultured in a humidified incubator (95% air with 5% CO2) at 37 °C with a complete medium designed for optimal growth of endothelial cells in vitro. A 6 mm thick PDMS containingFull Paper
2018Yasinska, IM;Gonçalves Silva, I;Sakhnevych, SS;Ruegg, L;Hussain, R;Siligardi, G;Fiedler, W;Wellbrock, J;Bardelli, M;Varani, L;Raap, U;Berger, S;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV;High mobility group box 1 (HMGB1) acts as an "alarmin" to promote acute myeloid leukaemia progressionOncoimmunology29872582lemented with 10% foetal bovine serum, penicillin (50 IU/ml) and streptomycin sulphate (50 µg/ml). Primary human AML mononuclear blasts (AML-PB001 F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordancFull Paper
2018Lam, J;Bellayr, IH;Marklein, RA;Bauer, SR;Puri, RK;Sung, KE;Functional Profiling of Chondrogenically Induced Multipotent Stromal Cell Aggregates Reveals Transcriptomic and Emergent Morphological Phenotypes Predictive of Differentiation CapacityStem cells translational medicine30084545rived MSCs were obtained from eight different donors purchased from RoosterBio (RB9, RB12, RB14, RB16; Frederick, MD) at passage 1 (P1), Lonza (8F560, 167696; Walkersville, MD) at passage 2 (P2), and AllCells (PCBM1632, PCBM1641; Alameda, CA) at P2 (Full Paper
2018George, MJ;Prabhakara, K;Toledano-Furman, NE;Wang, YW;Gill, BS;Wade, CE;Olson, SD;Cox, CS;Clinical Cellular Therapeutics Accelerate Clot FormationStem cells translational medicine30070065Bone marrow derived MSCs (BM MSCs) were isolated from commercially available fresh human bone marrow aspirates (AllCells, Emeryville, CA) or obtained from fresh bone marrow SaveFull Paper
2018Marcellino, BK;Hoffman, R;Tripodi, J;Lu, M;Kosiorek, H;Mascarenhas, J;Rampal, RK;Dueck, A;Najfeld, V;Advanced forms of MPNs are accompanied by chromosomal abnormalities that lead to dysregulation of TP53Blood advances30563882Normal bone marrow MNCs were obtained from AllCells (Alameda, CA). RNA was extracted from MNCs, using RNeasy mini kit (Fisher Scientific, Waltham, MA), and was reverse SaveFull Paper
2018Wei, Y;Zheng, H;Bao, N;Jiang, S;Bueso-Ramos, CE;Khoury, J;Class, C;Lu, Y;Lin, K;Yang, H;Ganan-Gomez, I;Starczynowski, DT;Do, KA;Colla, S;Garcia-Manero, G;KDM6B overexpression activates innate immune signaling and impairs hematopoiesis in miceBlood advances30275007BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). CD34 + cells were isolated using MicroBead Kit (Miltenyi Biotec). For colony formation, BM CD34 + SaveFull Paper
2018Witt, RG;Kreger, EM;Buckman, LB;Moradi, PW;Ho, PT;Derderian, SC;Tsai, P;Baker, C;Schramm, N;Cleary, R;Garcia, JV;MacKenzie, TC;Systemic multilineage engraftment in mice after in utero transplantation with human hematopoietic stem cellsBlood advances29344586Human cord blood CD34 + cells were either purchased from AllCells (Alameda, CA) or purified from cord blood collected at time of delivery from normal-term infants. CD34 + cells were SaveFull Paper
2018Murray, C;Miwa, H;Dhar, M;Park, DE;Pao, E;Martinez, J;Kaanumale, S;Loghin, E;Graf, J;Raddassi, K;Kwok, WW;Hafler, D;Puleo, C;Di Carlo, D;Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratchetingLab on a chip30039125Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) were purchased from ALLCELLs (Alameda, CA, USA; Cat No. PB003F) and stored at −180°C in vapor phase liquid nitrogen until ready for experimental use. Cells were thawed by placing cryovial in 37Full Paper
2018Romero-Masters, JC;Ohashi, M;Djavadian, R;Eichelberg, MR;Hayes, M;Bristol, JA;Ma, S;Ranheim, EA;Gumperz, J;Johannsen, EC;Kenney, SC;An EBNA3C-deleted Epstein-Barr virus (EBV) mutant causes B-cell lymphomas with delayed onset in a cord blood-humanized mouse modelPLoS pathogens30125329Commercially available CD34-depleted human cord blood mononuclear cells (AllCells) were infected with 5000 Green Raji Units of wild-type or mutant EBV strains in vitro for 1 ½ hours SaveFull Paper
2018Miller, AZ;Satchie, A;Tannenbaum, AP;Nihal, A;Thomson, JA;Vereide, DT;Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal TransitionStem cell reports29320761scribed in Figure 5 as a means to transiently label transfected cells. Sources of Primary Cells Frozen primary CD34+ cells purified from umbilical cord blood or adult bone marrow were obtained from AllCells (for cord blood, products CB005F and CB009Full Paper
2018Tsai, HC;Velichko, S;Lee, S;Wu, R;Cholera toxin enhances interleukin-17A production in both CD4+ and CD8+ cells via a cAMP/protein kinase A-mediated interleukin-17A promoter activationImmunology29377102Peripheral blood mononuclear cells were purchased from Allcells, LLC (Alameda, CA) or isolated from the leukopak filters from the Delta Blood Bank in Stockton, CA. Total CD4 + T SaveFull Paper
2018Yang, R;Liu, Y;Yu, T;Liu, D;Shi, S;Zhou, Y;Zhou, Y;Hydrogen sulfide maintains dental pulp stem cell function via TRPV1-mediated calcium influxCell death discovery3006205000 μg/ml streptomycin and passaged, as previously reported27. Human bone marrow MSCs BMMSC culture Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells (Emeryville, CA, USA). Human bone marrow progeFull Paper
2018Zhou, Z;Shao, T;Qin, M;Miao, X;Chang, Y;Sheng, W;Wu, F;Yu, Y;The effects of autophagy on vascular endothelial cells induced by airborne PM2.5Journal of environmental sciences (China)29628085In this study, human umbilical vein endothelial cells (HUVECs) were obtained from Allcells, China. The cells were routinely maintained in an HUVEC complete culture medium (Allcells, SaveFull Paper
2018Figueroa, I;Leipold, D;Leong, S;Zheng, B;Triguero-Carrasco, M;Fourie-O'Donohue, A;Kozak, KR;Xu, K;Schutten, M;Wang, H;Polson, AG;Kamath, AV;Prediction of non-linear pharmacokinetics in humans of an antibody-drug conjugate (ADC) when evaluation of higher doses in animals is limited by tolerability: Case study with an anti-CD33 ADCmAbs2975769861 Samples included normal human whole blood donors (n = 6 from Genentech, Inc. South San Francisco, CA; for phenotyping lymphocyte and myeloid subsets), normal human bone marrow donors (n = 5 from ALLCells, Alameda, CA; for phenotyping hematopoieticFull Paper
2018Jiang, N;Wang, Y;Yin, Y;Wei, R;Ying, G;Li, B;Qiu, T;van Rijn, P;Tian, G;Yan, Q;Dai, H;Busscher, H;Li, S;Yetisen, A;Yang, X;bFGF and Poly-RGD Cooperatively Establish Biointerface for Stem Cell Adhesion, Proliferation, and DifferentiationAdvanced Materials InterfaceshBMMSCs Culture: Freshly isolated human bone marrow mononuclear cells obtained from a 23-year-old donor were purchased from ALLCELLS (Emeryville, CA, US), and cultured with α-minimum essential media (MEM)/15% FBS/glutamine (2 mmol L−1)/penicillin (10Full Paper
2018Hoff, FW;Hu, CW;Qiu, Y;Ligeralde, A;Yoo, SY;Mahmud, H;de Bont, ESJM;Qutub, AA;Horton, TM;Kornblau, SM;Recognition of Recurrent Protein Expression Patterns in Pediatric Acute Myeloid Leukemia Identified New Therapeutic TargetsMolecular cancer research : MCR29669821patients (57 pre-B ALL and 16 T-ALL, patient demographics are presented in Supplemental Table S2), 10 cryopreserved normal BM CD34+ (AllCells, Almeda CA), and 127 leukemic cell line samples. The methods and validation of the technique, as well as theFull Paper
2018Bates, AM;Fischer, CL;Abhyankar, VP;Johnson, GK;Guthmiller, JM;Progulske-Fox, A;Brogden, KA;Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-BInternational journal of molecular sciences30544510ls, GE keratinocytes, and T-cells were chosen for this experiment due to their presence in the periodontium and their innate immune functions. Human monocyte-derived immature myeloid dendritic cells (AllCells, Alameda, CA, USA) were thawed, washed wiFull Paper
2018Chen, Q;Smith, S;Fong, T;Shirazian, A;Lee, J;Chapman, L;Tong, J;Vaz, W;Practical considerations for sourcing clinical-grade human tissue to support development and production of emerging commercial cellular therapiesCytotherapyQ. Chen, S. Smith, T. Fong, A. Shirazian, J. Lee, L. Chapman, J. Tong, W. Vaz Commercial, AllCells, LLC, Alameda, California, United States The rapid clinical advancement and commercial manufacturing of cellular therapeutics pose numerous industrialFull Paper
2018Okragly, AJ;Morin, SM;DeRosa, D;Martin, AP;Johnson, KW;Johnson, MP;Benschop, RJ;Human mast cells release the migraine-inducing factor pituitary adenylate cyclase-activating polypeptide (PACAP)Cephalalgia : an international journal of headache29103295Human umbilical cord blood-derived mast cells were produced by in vitro differentiation of positively selected cord blood CD34+ progenitor cells (Allcells) as previously described (37,38). Cells were cultured in Serum Free Expansion Media (SFEM Cat.Full Paper
2018Oberst, MD;Augé, C;Morris, C;Kentner, S;Mulgrew, K;McGlinchey, K;Hair, J;Hanabuchi, S;Du, Q;Damschroder, M;Feng, H;Eck, S;Buss, N;de Haan, L;Pierce, AJ;Park, H;Sylwester, A;Axthelm, MK;Picker, L;Morris, NP;Weinberg, A;Hammond, SA;Potent Immune Modulation by MEDI6383, an Engineered Human OX40 Ligand IgG4P Fc Fusion ProteinMolecular cancer therapeutics29545330Ligation of OX40 (CD134, TNFRSF4) on activated T cells by its natural ligand (OX40L, CD252, TNFSF4) enhances cellular survival, proliferation, and effector functions such as cytokine SaveFull Paper
2018Fujisawa, K;Takami, T;Okada, S;Hara, K;Matsumoto, T;Yamamoto, N;Yamasaki, T;Sakaida, I;Analysis of Metabolomic Changes in Mesenchymal Stem Cells on Treatment with Desferrioxamine as a Hypoxia Mimetic Compared with Hypoxic ConditionsStem cells (Dayton, Ohio)29577517Cells and Cell Culturing BMSCs were obtained from bone marrow cells (age: 34, male) purchased from AllCells (Alameda, CA), and BMSCs were proliferated using passage 4 and passage 16. BMSCs were confirmed to be CD45 (-), CD11b (-), CD90 (+), CD73 (+),Full Paper
2018Fujii, S;Miura, Y;Fujishiro, A;Shindo, T;Shimazu, Y;Hirai, H;Tahara, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T;Graft-Versus-Host Disease Amelioration by Human Bone Marrow Mesenchymal Stromal/Stem Cell-Derived Extracellular Vesicles Is Associated with Peripheral Preservation of Naive T Cell PopulationsStem cells (Dayton, Ohio)29239062Human MSCs were isolated from BM samples purchased from AllCells (Alameda, CA) using our previously published method [21, 22]. BM samples were obtained from three different SaveFull Paper
2018Harting, MT;Srivastava, AK;Zhaorigetu, S;Bair, H;Prabhakara, KS;Toledano Furman, NE;Vykoukal, JV;Ruppert, KA;Cox, CS;Olson, SD;Inflammation-Stimulated Mesenchymal Stromal Cell-Derived Extracellular Vesicles Attenuate InflammationStem cells (Dayton, Ohio)29076623Human mesenchymal stromal cells (hMSCs) were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using SaveFull Paper
2018Meader, E;Barta, T;Melguizo-Sanchis, D;Tilgner, K;Montaner, D;El-Harouni, AA;Armstrong, L;Lako, M;Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAsStem cells (Dayton, Ohio)29047185and TaqMan MicroRNA Assays were used for the qRT‐PCR (all from Thermo Fisher Scientific) RNU44 and RNU48 were chosen as internal controls. CD34+ human adult bone marrow and CD34+ cord blood cells (AllCells, Alameda, CA, USA) were used as positive conFull Paper
2018Wang, J;Li, T;Feng, J;Li, L;Wang, R;Cheng, H;Yuan, Y;Kaempferol protects against gamma radiation-induced mortality and damage via inhibiting oxidative stress and modulating apoptotic molecules in vivo and vitroEnvironmental toxicology and pharmacology29705372To investigate the potential protective effect of kaempferol, a representative flavonoid, against radiation induced mortality and injury in vivo and vitro.C57BL/6 male mice and human SaveFull Paper
2018Garbuzova-Davis, S;Haller, E;Navarro, S;Besong, TE;Boccio, KJ;Hailu, S;Khatib, M;Sanberg, PR;Appel, SH;Borlongan, CV;Transplantation of human bone marrow stem cells into symptomatic ALS mice enhances structural and functional blood-spinal cord barrier repairExperimental neurology30172620Accumulating evidence shows alterations in the blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) in ALS patients and in animal models of disease, mainly by endothelial SaveFull Paper
2018Huang, L;Zhang, X;Ma, X;Zhang, D;Li, D;Feng, J;Pan, X;Lü, J;Wang, X;Liu, X;Berberine alleviates endothelial glycocalyx degradation and promotes glycocalyx restoration in LPS-induced ARDSInternational immunopharmacology30308440(Shanghai, China) and grown to confluence in 25 cm 2 flasks supplemented with complete culture medium, which was provided by AllCells. The cells were incubated under 95% SaveFull Paper
2018Romero, Z;DeWitt, M;Walters, MC;Promise of gene therapy to treat sickle cell diseaseExpert opinion on biological therapy30324810MC Walters is the Medical Director of AllCells, Inc and ViaCord Processing Lab; Consultant for Sangamo Therapeutics, INc, bluebird bio, Inc., and Bioverativ, Inc. The authors have no other relevant affiliations or financial involvement with any organFull Paper
2018Hu, Y;Yao, X;Liu, Q;Wang, Y;Liu, R;Cui, S;Ding, J;Left-Right Symmetry or Asymmetry of Cells on Stripe-Like Micropatterned Material SurfacesChinese Journal of ChemistryrMSCs were bought from Allcells Biological Technology, Shanghai. hMSCs were from Cyagen Biosciences, Suzhou. HFFs and HUVECs were products of Bogoo Biological Technology, Shanghai. The cells of rMSCs were incubated in minimum essential medium α (MEMFull Paper
2018Yuan, X;Han, L;Fu, P;Zeng, H;Lv, C;Chang, W;Runyon, RS;Ishii, M;Han, L;Liu, K;Fan, T;Zhang, W;Liu, R;Cinnamaldehyde accelerates wound healing by promoting angiogenesis via up-regulation of PI3K and MAPK signaling pathwaysLaboratory investigation; a journal of technical methods and pathology29463877Cell culture and animal maintenance Primary HUVECs were purchased from AllCells (Emeryville, CA, USA). With the exception of the homocysteine-rescue tube assay, each batch of HUVECs was examined using flow cytometry and was shown to be 99% CD31 positFull Paper
2018Huang, T;Mathieu, M;Lee, S;Wang, X;Kee, YS;Bevers, JJ;Ciferri, C;Estavez, A;Wong, M;Chiang, NY;Nakamura, G;Brezski, RJ;Molecular characterization of human anti-hinge antibodies derived from single-cell cloning of normal human B cellsThe Journal of biological chemistry29191832PBMCs were isolated from a Leuko Pak (AllCells) using Ficoll-Paque gradient fractionation (GE Healthcare). PBMCs were then frozen in 90% fetal bovine serum (FBS) supplemented with 10% dimethyl sulfoxide (DMSO) and stored in liquid nitrogen until thawFull Paper
2018Smith, AJ;Graves, B;Child, R;Rice, PJ;Ma, Z;Lowman, DW;Ensley, HE;Ryter, KT;Evans, JT;Williams, DL;Immunoregulatory Activity of the Natural Product Laminarin Varies Widely as a Result of Its Physical PropertiesJournal of immunology (Baltimore, Md. : 1950)29246954PBMCs obtained via leukapheresis were purchased from AllCells (Alameda, CA) and cryopreserved in 50% RPMI 1640 with 40% FBS/10% DMSO, after extensive washing in 1× PBS. SaveFull Paper
2018Yang, C;Huan, Z;Wang, X;Wu, C;Chang, J;3D Printed Fe Scaffolds with HA Nanocoating for Bone RegenerationACS biomaterials science & engineering33418749initial density of 1× 104 rabbit bone marrow mesenchymal stem cells (rBMSCs) (Allcells, Silicon Valley, CA, USA) in 48-well tissue culture plates and then incubated at 37 °C wiht 5% CO2. After 24 h, the cell culture medium was changed for the first tFull Paper
2018Piao, XM;Gao, F;Zhu, JX;Wang, LJ;Zhao, X;Li, X;Sheng, MM;Zhang, Y;Cucurbitacin B inhibits tumor angiogenesis by triggering the mitochondrial signaling pathway in endothelial cellsInternational journal of molecular medicine29717773HUVECs and HepG2 cells were obtained from Allcells, LLC (Shanghai, China). CuB (purity 99%) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China). DMEM/F-12, fetal bovine serum (FBS), was from Gibco (Carlsbad, CA, USA). EGM-2 complete mediFull Paper
2018Zhu, H;Yu, B;Yang, L;Exogenous and Endogenous Stem Cells for Skeletal RegenerationStem cells international30581472HUVECs were purchased from AllCells, Ltd. (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth factors [19]. To investigate the SaveFull Paper
2018Zhang, M;Yu, W;Niibe, K;Zhang, W;Egusa, H;Tang, T;Jiang, X;The Effects of Platelet-Derived Growth Factor-BB on Bone Marrow Stromal Cell-Mediated Vascularized Bone RegenerationStem cells international30515221HUVECs were purchased from AllCells, Ltd. (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth factors [19]. To investigate the SaveFull Paper
2018Kwak, J;Choi, SJ;Oh, W;Yang, YS;Jeon, HB;Jeon, ES;Cobalt Chloride Enhances the Anti-Inflammatory Potency of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells through the ERK-HIF-1α-MicroRNA-146a-Mediated Signaling PathwayStem cells international30254683103 cells/well) were seeded and maintained at 37°C in a humidified incubator for 2-4 hr and then cocultured with responder peripheral blood mononuclear cells (PBMCs; 1 × 105 cells/well; AllCells, Boston, MA, USA) and stimulator PBMCs (1 × 105 cells/Full Paper
2018Chen, B;Ni, Y;Liu, J;Zhang, Y;Yan, F;Bone Marrow-Derived Mesenchymal Stem Cells Exert Diverse Effects on Different Macrophage SubsetsStem cells international30140291y directed activation. Western blot was used to detect the expression of iNOS and Arg-1 in the cells to further verify the M1 and M2 polarization. 2.5. Preparation of MSC-CM BMMSC was purchased from AllCells, LLC (Emeryville, CA), and MSCs at passagFull Paper
2018Jiang, J;Xu, K;Wang, L;Xin, W;Zhao, G;Huang, M;Li, S;Luan, X;Fang, J;Pharmacology study of a chimeric decoy receptor trap fusion protein on retina neovascularization by dual blockage of VEGF and FGF-2European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences29715501Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from AllCells (Shanghai, China) and maintained in a complete HUVEC medium (Cat# H-004, AllCells)Full Paper
2018Yen, J;Fiorino, M;Liu, Y;Paula, S;Clarkson, S;Quinn, L;Tschantz, WR;Klock, H;Guo, N;Russ, C;Yu, VWC;Mickanin, C;Stevenson, SC;Lee, C;Yang, Y;TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem CellsScientific reports30389991Cryopreserved bone marrow derived CD34 + HSPCs were purchased from Lonza or AllCells and thawed according to vendors' instructions. Cells were cultured at 37 C 5% CO 2 in SaveFull Paper
2018Han, SS;Shim, HE;Park, SJ;Kim, BC;Lee, DE;Chung, HM;Moon, SH;Kang, SW;Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for TrackingScientific reports30181604The protocol was approved by the Committee on Biological Research of Korea Institute of Toxicology and Institutional Review Board (P01-201509-41-001). hUCB-EPCs culture hUCB-EPCs were purchased from AllCells (Alameda, CA, USA). hUCB-EPCs culture werFull Paper
2018Burgenson, D;Gurramkonda, C;Pilli, M;Ge, X;Andar, A;Kostov, Y;Tolosa, L;Rao, G;Rapid recombinant protein expression in cell-free extracts from human bloodScientific reports29934577anced the expression of Nluc, but RNase inhibitor did not have a significant effect (Fig. 2C,D).Figure 1Production of human leukocyte extract and Nluc expression. (A) Leukopak was purchased form the AllCells, Inc. (B) The cells were clarified by centFull Paper
2018Valton, J;Guyot, V;Boldajipour, B;Sommer, C;Pertel, T;Juillerat, A;Duclert, A;Sasu, BJ;Duchateau, P;Poirot, L;A Versatile Safeguard for Chimeric Antigen Receptor T-Cell ImmunotherapiesScientific reports29895885Primary T-cells isolated from PBMC (ALLCELLS) were activated and transfected according to previously described methods 13 . Four days after their activation by Dynabeads human T SaveFull Paper
2018Gaudin, A;Tolar, M;Peters, OA;Lipoxin A4 Attenuates the Inflammatory Response in Stem Cells of the Apical Papilla via ALX/FPR2Scientific reports29892010eated with mitomycin C (Sigma-Aldrich) at the final concentration of 25 μg/mL for 30 min, and then washed 4 times with PBS + 10% Human Serum. Human peripheral blood mononuclear cells (PBMC) (AllCells, Alameda, CA, USA) in dilution ratios 1:1, 1:5 andFull Paper
2018Lim, SH;Sun, Y;Thiruvallur Madanagopal, T;Rosa, V;Kang, L;Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular ModificationScientific reports29371611215 nm. A calibration curve was conducted using the respective standard solutions from 1 ppm to 1000 ppm. DPSC Cell Culture Primary human dental pulp stem cells (DPSC) from a single donor (DPF003, AllCells, USA) were cultured in a complete growth meFull Paper
2018Ruppert, KA;Nguyen, TT;Prabhakara, KS;Toledano Furman, NE;Srivastava, AK;Harting, MT;Cox, CS;Olson, SD;Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Modify Microglial Response and Improve Clinical Outcomes in Experimental Spinal Cord InjuryScientific reports29323194hMSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old male (AllCells, Alameda, CA) using density centrifugation and plastic adherence SaveFull Paper
2018Li, C;Sunderic, K;Nicoll, SB;Wang, S;Downregulation of Heat Shock Protein 70 Impairs Osteogenic and Chondrogenic Differentiation in Human Mesenchymal Stem CellsScientific reports29323151Human MSCs were isolated from fresh human bone marrow purchased from AllCells (Alameda, CA) according to manufacturer's instructions as previously described 2 and cultured SaveFull Paper
2018Bloom, DD;Reshetylo, S;Nytes, C;Goodsett, CT;Hematti, P;Blockade of BAFF Receptor BR3 on T Cells Enhances Their Activation and CytotoxicityJournal of immunotherapy (Hagerstown, Md. : 1997)29309290Primary human T cells were obtained from leukopheresis products purchased from AllCells, LLC (Alameda, CA) or Key Biologics, LLC (Memphis, TN). Upon arrival, PBLs were isolated SaveFull Paper
2018Mair, F;Prlic, M;OMIP-044: 28-color immunophenotyping of the human dendritic cell compartmentCytometry. Part A : the journal of the International Society for Analytical Cytology29356334Cryopreserved PBMCs were obtained from AllCells, stained and subsequently aquired on a BD FACSymphony. Optimal PMT voltages were determined by voltage titration experiments and iterative refinement. All reagents were titrated prior to use. For FigureFull Paper
2018Doering, CB;Denning, G;Shields, JE;Fine, EJ;Parker, ET;Srivastava, A;Lollar, P;Spencer, HT;Preclinical Development of a Hematopoietic Stem and Progenitor Cell Bioengineered Factor VIII Lentiviral Vector Gene Therapy for Hemophilia AHuman gene therapy30160169Genetically modified, autologous hematopoietic stem and progenitor cells (HSPCs) represent a new class of genetic medicine. Following this therapeutic paradigm, we are developing a SaveFull Paper
2018Liu, XY;Xu, HX;Li, JK;Zhang, D;Ma, XH;Huang, LN;Lü, JH;Wang, XZ;Neferine Protects Endothelial Glycocalyx via Mitochondrial ROS in Lipopolysaccharide-Induced Acute Respiratory Distress SyndromeFrontiers in physiology29520236Human umbilical vein endothelial cells (HUVECs) were grown to confluence and cultured in 25 cm 2 flasks supplemented with complete culture medium (AllCells, Shanghai, China) in a SaveFull Paper
2018Zeng, Y;Dabay, M;George, V;Seetharaman, S;de Arruda Indig, M;Graminske, S;Kimpel, N;Schmidt, A;Boerner, A;Paradiso, S;Delman, T;Li, Y;Litvak, V;Oreizy, F;Chen, A;Saleminik, M;Mosqueda, F;Lin, A;Judge, K;Comparison of Flow Cytometric Methods for the Enumeration of Residual Leucocytes in Leucoreduced Blood Products: A Multicenter StudyCytometry. Part A : the journal of the International Society for Analytical Cytology29345745Per enrollment needs, leucoreduced PLTs and RBCs with autologous white blood cell sources were also purchased from qualified vendors that produce apheresis PLTs and RBCs through standard procedures (AllCells, LLC, Alameda, CA; Stanford Blood Center,Full Paper
2018Song, B;Chen, Y;Liu, Y;Wan, C;Zhang, L;Zhang, W;NPAS2 regulates proliferation of acute myeloid leukemia cells via CDC25A-mediated cell cycle progression and apoptosisJournal of cellular biochemistry30536616NHC cell specimens were separated from normal cells in bone marrow bought from AllCells, LLC (Emeryville, CA). Cells were preserved in 5% CO 2 at 37C in RPMI-1640 media SaveFull Paper
2018Lewis, G;Christiansen, L;McKenzie, J;Luo, M;Pasackow, E;Smurnyy, Y;Harrington, S;Gregory, P;Veres, G;Negre, O;Bonner, M;Staurosporine Increases Lentiviral Vector Transduction Efficiency of Human Hematopoietic Stem and Progenitor CellsMolecular therapy. Methods & clinical development30038935CD34-enriched mPB samples from healthy human donors were obtained from AllCells (Emeryville, CA) and Key Biologics (Memphis, TN). Research-grade staurosporine was obtained Save;apeutics. Materials and Methods Cell Culture Research-grade BlaM-contaFull Paper
2018Mendoza, M;Ballesteros, A;Qiu, Q;Pow Sang, L;Shashikumar, S;Casares, S;Brumeanu, TD;Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD)Human vaccines & immunotherapeutics29135340Briefly, HLA-DRB1*0401 + umbilical cord bloods (UCB) purchased from AllCells and Promocell were enriched for CD34 + stem cells to more than 60% when using EasySep, Human SaveFull Paper
2018Low, EK;Brudvik, E;Kuhlman, B;Wilson, PF;Almeida-Porada, G;Porada, CD;Microgravity Impairs DNA Damage Repair in Human Hematopoietic Stem/Progenitor Cells and Inhibits Their Differentiation into Dendritic CellsStem cells and development29901426Healthy human BM mononuclear cells (BMMNC) were purchased from AllCells, LLC (Alameda, CA). CD34+ HSC were isolated from BMMNC using anti-human CD34 MicroBeads and the MiniMACS magnetic-based positive-selection system (Miltenyi Biotec, Inc., Auburn,Full Paper
2018Almeida-Porada, G;Rodman, C;Kuhlman, B;Brudvik, E;Moon, J;George, S;Guida, P;Sajuthi, SP;Langefeld, CD;Walker, SJ;Wilson, PF;Porada, CD;Exposure of the Bone Marrow Microenvironment to Simulated Solar and Galactic Cosmic Radiation Induces Biological Bystander Effects on Human HematopoiesisStem cells and development29698131In brief, upon return from BNL, MSC from each irradiation group were overlaid with human HSC (CD34+ BM-derived cells from healthy adult donors, purchased from AllCells, LLC, or SaveFull Paper
2018Sebastian, AA;Kannan, TP;Norazmi, MN;Nurul, AA;Interleukin-17A promotes osteogenic differentiation by increasing OPG/RANKL ratio in stem cells from human exfoliated deciduous teeth (SHED)Journal of tissue engineering and regenerative medicine29774992SHED were purchased from ALLCELLS, Alameda, CA, USA (Catalogue No. DP004F; lot number SHED092211-01). SHED from Passages 4 to 6 were used in the study. SHED were cultured in alpha minimum essential medium (Gibco, USA) supplemented with 10% v/v fetalFull Paper
2018Gregg, KA;Harberts, E;Gardner, FM;Pelletier, MR;Cayatte, C;Yu, L;McCarthy, MP;Marshall, JD;Ernst, RK;A lipid A-based TLR4 mimetic effectively adjuvants a Yersinia pestis rF-V1 subunit vaccine in a murine challenge modelVaccine29861179Co-stimulatory marker surface expression assays were contracted to and completed by AllCells using standard protocols. Briefly, ex vivo human monocytes were differentiated to SaveFull Paper
2018Kanagasabai, R;Karmahapatra, S;Kientz, CA;Yu, Y;Hernandez, VA;Kania, EE;Yalowich, JC;Elton, TS;The Novel C-terminal Truncated 90-kDa Isoform of Topoisomerase IIα (TOP2α/90) Is a Determinant of Etoposide Resistance in K562 Leukemia Cells via Heterodimerization with the TOP2α/170 IsoformMolecular pharmacology29514855Human cord blood mononuclear cells were purchased from ALLCELLS (Alameda, CA). Deidentified acute myeloid leukemia (AML) patient samples were obtained from The Ohio State SaveFull Paper
2018Liu, L;Puri, N;Raffaele, M;Schragenheim, J;Singh, SP;Bradbury, JA;Bellner, L;Vanella, L;Zeldin, DC;Cao, J;Abraham, NG;Ablation of soluble epoxide hydrolase reprogram white fat to beige-like fat through an increase in mitochondrial integrity, HO-1-adiponectin in vitro and in vivoProstaglandins & other lipid mediators30041041Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA, USA). After thawing, mononuclear cells were re-suspended in a-minimal essential medium (a-MEM, Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat-in-aFull Paper
2018Wang, J;Xu, J;Peng, Y;Xiao, Y;Zhu, H;Ding, ZM;Hua, H;Phosphorylation of extracellular signal-regulated kinase as a biomarker for cannabinoid receptor 2 activationHeliyon30450437tal calf serum, 100 U/ml penicillin/streptomycin, and 2 mM l-glutamine at 37 °C in 5% CO2/95% air. 2.2 Human whole blood cell culture Human whole blood cells were purchased from commercial sources (Allcells, Alameda, CA, USA or Zen-Bio, Research TriFull Paper
2018Wiredja, DD;Tabler, CO;Schlatzer, DM;Li, M;Chance, MR;Tilton, JC;Global phosphoproteomics of CCR5-tropic HIV-1 signaling reveals reprogramming of cellular protein production pathways and identifies p70-S6K1 and MK2 as HIV-responsive kinases required for optimal infection of CD4+ T cellsRetrovirology29970186Normal donor samples were de-identified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated by SaveFull Paper
2018Friesen, WJ;Johnson, B;Sierra, J;Zhuo, J;Vazirani, P;Xue, X;Tomizawa, Y;Baiazitov, R;Morrill, C;Ren, H;Babu, S;Moon, YC;Branstrom, A;Mollin, A;Hedrick, J;Sheedy, J;Elfring, G;Weetall, M;Colacino, JM;Welch, EM;Peltz, SW;The minor gentamicin complex component, X2, is a potent premature stop codon readthrough molecule with therapeutic potentialPloS one30359426). Fold suppression over background was calculated as (aminoglycoside light units/PBS light units) [7]. Cytotoxicity assays Frozen human Peripheral Blood Mononuclear Cells (PBMCs) were obtained from ALLCELLS (Alameda, CA, Cat# PB003F) from a singleFull Paper
2018Caseiro, AR;Ivanova, G;Pedrosa, SS;Branquinho, MV;Georgieva, P;Barbosa, PP;Santos, JD;Magalhães, R;Teixeira, P;Pereira, T;Maurício, AC;Human umbilical cord blood plasma as an alternative to animal sera for mesenchymal stromal cells in vitro expansion - A multicomponent metabolomic analysisPloS one30304014, 29, 47-49]. UC-MSCs and DPSCs were maintained at 37°C and 95% humidified atmosphere with 5% CO2 environment. UC-MSCs were obtained from PromoCell, Cat. C-12971; Lot No. 1112304.2) and DPSCs from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01).Full Paper
2018Oshima, T;Miyashita, H;Ishimura, Y;Ito, Y;Tanaka, Y;Hori, A;Kokubo, T;Kurokawa, T;Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkeyPloS one29723247Human peripheral blood mononuclear cells (PBMC) purchased from AllCells, LLC and were cultured in RPMI1640 medium containing 10% fetal bovine serum, 0.1 nM human IL-2 ( SaveFull Paper
2018Del'Guidice, T;Lepetit-Stoffaes, JP;Bordeleau, LJ;Roberge, J;Théberge, V;Lauvaux, C;Barbeau, X;Trottier, J;Dave, V;Roy, DC;Gaillet, B;Garnier, A;Guay, D;Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cellsPloS one29617431N). Myoblasts are primary human cells kindly provided by Professor J.P. Tremblay (Université Laval, Quebec, QC, Canada). Normal Peripheral Blood CD56+ NK cells are natural killer cells obtained from Allcells Inc. (Alameda, CA, USA). Cortical cells arFull Paper
2018Sunderic, K;Effects of Thermal Dosing and Ultrasound on Osteogenic Differentiation of Human Mesenchymal Stem Cells in 2D and 3D CultureThesisHuman bone marrow explanted from the iliac crest of donors was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the RosetteSep MSC enrichment cocktail (StemCell Technologies, Vancouver, Canada) per manufacFull Paper
2018Nair, RR;Piktel, D;Geldenhuys, WJ;Gibson, LF;Combination of cabazitaxel and plicamycin induces cell death in drug resistant B-cell acute lymphoblastic leukemiaLeukemia research30103201Primary immune cells CD3 + T cells, CD19 + B cells, peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) were all purchased from ALLCELLS and SaveFull Paper
2018Palasuk, J;Windsor, LJ;Platt, JA;Lvov, Y;Geraldeli, S;Bottino, MC;Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashionClinical oral investigations28965247Next, the eluates were serially diluted to 32-, 64-, and 128-fold [21]. Human dental pulp stem cells ([hDPSCs] AllCells LLC, Alameda, CA, USA) were cultured under standard conditions in a humidifier incubator (5% CO2/95% air) at 37 °C in low-glucoseFull Paper
2018Sasaki, E;Momose, H;Hiradate, Y;Ishii, KJ;Mizukami, T;Hamaguchi, I;In vitro marker gene expression analyses in human peripheral blood mononuclear cells: A tool to assess safety of influenza vaccines in humansJournal of immunotoxicology29521144PBMC obtained from healthy adult subjects were purchased from AllCells (Alameda, CA) and cultured in complete RPMI, ie RPMI 1640 supplemented with 10% fetal calf serum (FCS), SaveFull Paper
2018Tian, H;Li, S;Yu, K;DJ‑1 alleviates high glucose‑induced endothelial cells injury via PI3K/Akt‑eNOS signaling pathwayMolecular medicine reports29115508HUVECs were purchased from AllCells Biotechnology Co., Ltd. (Shanghai, China). The culture medium was DMEM with either 5.5 mM (control group) or 25 mM (HG group) D-glucose, containing 10% fetal bovine serum, penicillin (100 U/ml) and streptomycin (10Full Paper
2018Hu, X;Hu, T;Guan, G;Yu, S;Wu, Y;Wang, L;Control of weft yarn or density improves biocompatibility of PET small diameter artificial blood vesselsJournal of biomedical materials research. Part B, Applied biomaterials28456004The HUVECs (HUVEC001), provided by Allcells Company (Shanghai, China), were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Gibco) at 37°C in a 5% CO2 incubator. Before seFull Paper
2018Joslin, J;Gilligan, J;Anderson, P;Garcia, C;Sharif, O;Hampton, J;Cohen, S;King, M;Zhou, B;Jiang, S;Trussell, C;Dunn, R;Fathman, JW;Snead, JL;Boitano, AE;Nguyen, T;Conner, M;Cooke, M;Harris, J;Ainscow, E;Zhou, Y;Shaw, C;Sipes, D;Mainquist, J;Lesley, S;A Fully Automated High-Throughput Flow Cytometry Screening System Enabling Phenotypic Drug DiscoverySLAS discovery : advancing life sciences R & D29843542Primary human CD4 T cells were purified using a CD4+ T-cell isolation kit (130-096-533, Miltenyi, Auburn, CA) from cells obtained from leukapheresis of a normal human donor (AllCells Save;reg (T-Regulatory) Screen Primary human CD4 T cells were puriFull Paper
2018Barve, A;Casson, L;Krem, M;Wunderlich, M;Mulloy, JC;Beverly, LJ;Comparative utility of NRG and NRGS mice for the study of normal hematopoiesis, leukemogenesis, and therapeutic responseExperimental hematology30125602Cell-line-derived xenografts (CDXs) or patient-derived xenografts (PDXs) in immune-deficient mice have revolutionized our understanding of normal and malignant human SaveFull Paper
2018Reshetnikova, E;Guselnikov, S;Volkova, O;Baranov, K;Taranin, A;Mechetina, L;B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humansCytometry. Part B, Clinical cytometry29236355For this purpose, pDC cDNA from the BDCA4+ (CD304+) pDCs isolated from the peripheral blood of a healthy donor (cat. no. cDNA-PB006) was purchased from AllCells, LLC (Alameda, CA, USA). Human PBMC and spleen total RNA was isolated using Qiagen RNAeasFull Paper
2018Ikutani, M;Ogawa, S;Yanagibashi, T;Nagai, T;Okada, K;Furuichi, Y;Takatsu, K;Elimination of eosinophils using anti-IL-5 receptor alpha antibodies effectively suppresses IL-33-mediated pulmonary arterial hypertrophyImmunobiology29269115Human PBMCs (AllCells, Alameda, CA, USA) and Y16 cells were used as effector cells and target cells, respectively. Target cells (2 × 104 cells) were incubated with the effector cells (5 × 105 cells) and chimeric antibodies in a 96-well U-form plate aFull Paper
2018Sun, K;Mikule, K;Wang, Z;Poon, G;Vaidyanathan, A;Smith, G;Zhang, ZY;Hanke, J;Ramaswamy, S;Wang, J;A comparative pharmacokinetic study of PARP inhibitors demonstrates favorable properties for niraparib efficacy in preclinical tumor modelsOncotarget30647846m to assess human megakaryocyte-specific development In vitro human megakaryocyte differentiation was assessed using a liquid culture system in which the human bone marrow CD34+ cells (lot # 0150114, ReachBio) derived from normal bone marrow were useFull Paper
2018Liao, C;Regulation of Stress Erythropoiesis and the Niche: A Noval Role of SelenoproteinsThesisTransferrin (200µg/mL), Scf (50ng/mL), Bmp4 (15ng/mL), Gdf15 (30ng/mL), and Shh (25ng/mL), for 5 to 7 days to enrich for SEPs. Human BM cells from three subjects were purchased from Reachbio Research Labs. Human BM culture was the same condition withFull Paper
2018Wu, H;He, L;Shi, J;Hou, X;Zhang, H;Zhang, X;An, Q;Fan, F;Resveratrol inhibits VEGF-induced angiogenesis in human endothelial cells associated with suppression of aerobic glycolysis via modulation of PKM2 nuclear translocationClinical and experimental pharmacology & physiology30044005medium (DMEM) (Hyclone, Pittsburgh, PA, USA) supplemented with 10% foetal bovine serum (FBS; Cellmax, Beijing, China), 100 U/mL penicillin and 100 μg/mL streptomycin, and grown in a 95% air and 5% CO2 humidified atmosphere at 37°C, Primary HUVECs werFull Paper
2018Ni, R;Luo, L;A noncanonical function of histidyl-tRNA synthetase: inhibition of vascular hyperbranching during zebrafish developmentFEBS open bio29744287loheximide (CHX; Sigma) from 36 hpf to observation, or treated with DMSO at the same concentration as control. Immunofluorescence staining and imaging Human umbilical vein endothelial cells (HUVECs, ALLCELLS) were cultured according to standard protFull Paper
2018Han, XB;Tan, Y;Fang, YQ;Li, F;Protective effects of celastrol against γ irradiation-induced oxidative stress in human umbilical vein endothelial cellsExperimental and therapeutic medicine30116323anghai Xingya Purify Material Ltd. (Shanghai, China). Western blotting detection ECL Reagent was purchased from GE Healthcare China Co. Ltd. (Beijing, China). Cell culture HUVECs were purchased from AllCells Biotech Ltd. (Shanghai, China), and grownFull Paper
2018Varma, DM;DiNicolas, MS;Nicoll, SB;Injectable, redox-polymerized carboxymethylcellulose hydrogels promote nucleus pulposus-like extracellular matrix elaboration by human MSCs in a cell density-dependent mannerJournal of biomaterials applications30326804hMSCs were isolated from adult human bone marrow (AllCells, Emeryville, CA) via enriched density gradient centrifugation as previously described. Briefly, a hMSC Enrichment SaveFull Paper
2018Zhao, X;Gao, J;Hu, X;Guo, H;Wang, F;Qiao, Y;Wang, L;Collagen/Polyethylene Oxide Nanofibrous Membranes with Improved Hemostasis and Cytocompatibility for Wound DressingApplied SciencesHuman umbilical vein endothelial cells (HUVEC001) were supplied by Allcells Company (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, Shanghai, China) and 1% penicillin-streptomycin (Full Paper
2018Thomson-Luque, R;Wang, C;Ntumngia, FB;Xu, S;Szekeres, K;Conway, A;Adapa, SR;Barnes, SJ;Adams, JH;Jiang, RHY;In-depth phenotypic characterization of reticulocyte maturation using mass cytometryBlood cells, molecules & diseases30007855). Reticulocytes and mature RBCs were kept at 4 °C in MIM until used, always before 2 days post isolation. One bone marrow reticulocyte preparation was obtained from unprocessed whole aspirates (ALLCELLS) following the same protocol as for peripheralFull Paper
2018Wang, J;Xu, J;Liu, J;Zhu, H;Peng, Y;Ding, ZM;Hua, H;Genetic Variant Q63R of Cannabinoid Receptor 2 Causes Differential ERK Phosphorylation in Human Immune CellsGenetic testing and molecular biomarkers29694791All human PBMCs were from commercial sources (provided by Allcells or Zenbio). Human PBMCs were thawed and cultured in the culture medium (RPMI-1640 containing 2 mM L- SaveFull Paper
2018Gao, X;Yourick, JJ;Sprando, RL;Generation of nine induced pluripotent stem cell lines as an ethnic diversity panelStem cell research30099336Whole blood collection from healthy donors was conducted by AllCells with informed consent under the Protocol #7000-SOP-049 Version 2.0 approved by Alpha Independent Review SaveFull Paper
2018Pan, H;Zhang, M;Serum of coronary atherosclerotic heart disease patients induces oxidative stress injury on endothelial cellsPteridinesHUVECs were purchased from AllCells (AllCells Technology, Shanghai, China). Cells were grown in complete medium (HUVEC-004, AllCells) in an incubator with 5% CO2 at 37 °C. HUVECs were treated with the serum from patients with CAD for 24 h. HUVECs werFull Paper
2018Enezei, H;Ahmad, A;Takeuchi, K;Suzuki, J;Khamis, M;Razak, N;Rahman, R;Qabbani, A;Abdulhameed, E;Sugita, Y;Maeda, H;Alam, M;Osteoinductive Activity of Bone Scaffold Bioceramic Companied with Control Release of VEGF Protein Treated Dental stem cells as A New Concept for Bone Regeneration: Part IIJournal of Hard Tissue BiologyDental Stem Cells (DSCs) AllCells, LLC Company, Emeryville, CA, USA Dulbecco’s modified Eagles medium (DMEM) high glucose(4.5g/L) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Fetal bovine serum (FBS) Invitrogen, (GIBCO), Faraday Ave, Carlsbad,Full Paper
2018Sasaki, E;Momose, H;Hiradate, Y;Furuhata, K;Mizukami, T;Hamaguchi, I;Development of a preclinical humanized mouse model to evaluate acute toxicity of an influenza vaccineOncotarget29899819Human PBMCs from one healthy donor (24 years old, male adult Caucasian) were obtained from AllCells (Alameda, CA, USA) and were subjected to the experiment shown in Figures 1 SaveFull Paper
2018Gołąb, K;Grose, R;Placencia, V;Wickrema, A;Solomina, J;Tibudan, M;Konsur, E;Ciepły, K;Marek-Trzonkowska, N;Trzonkowski, P;Millis, JM;Fung, J;Witkowski, P;Cell banking for regulatory T cell-based therapy: strategies to overcome the impact of cryopreservation on the Treg viability and phenotypeOncotarget29515766We obtained de-identified human cell leukapheresis product for all the experiments from the commercial vendor AllCells, Alameda, CA, USA, therefore the study was exempt from the SaveFull Paper
2018Shehadat, SA;Gorduysus, MO;Hamid, SSA;Abdullah, NA;Samsudin, AR;Ahmad, A;Optimization of scanning electron microscope technique for amniotic membrane investigation: A preliminary studyEuropean journal of dentistry30369805que by a cell scraper (TPP, Europe/Switzerland) was applied when necessary to remove the epithelial cells that could not be washed away by phosphate-buffered saline (PBS). Deciduous teeth stem cells (AllCells, USA) were seeded on the de-epithelializeFull Paper
2018Subhi, H;Reza, F;Husein, A;Al Shehadat, SA;Nurul, AA;Gypsum-Based Material for Dental Pulp Capping: Effect of Chitosan and BMP-2 on Physical, Mechanical, and Cellular PropertiesInternational journal of biomaterials30147725SHED were purchased from AllCells, USA. The cells at passage 8 were cultured in Alpha minimum essential medium [????-MEM; Invitrogen, USA (GIBCO)] supplemented with 10% fetal SaveFull Paper
2018Ross, CL;Pettenati, MJ;Procita, J;Cathey, L;George, SK;Almeida-Porada, G;Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal CellsGlobal advances in health and medicine29796339me stability were not affected or altered by ELF-EMF, when used at the tested field strength and frequency. Materials and Methods Cell Culture Human bone mononuclear cells (BMNCs) were obtained from AllCells (Alameda, CA). BMNCs were enriched for thFull Paper
2018Subhi, H;Reza, F;Husein, A;Nurul, AA;Cytotoxicity of gypsum-based biomaterial for direct pulp capping using stem cells from human exfoliated deciduous teethJournal of conservative dentistry : JCD29628642epare the biomaterial. The compositions of the Gyp-CHT biomaterial are shown in Table 1. Table 1 The compositions of the experimental biomaterial Cell culture preparation SHED were purchased from AllCells, USA. The cells at passage eight were culFull Paper
2018Luddin, N;Ahmed, H;Kannan, T;Mokhtar, K;Ahmad, A;Calcium chloride dihydrate affects the biological properties of white mineral trioxide aggregate on dental pulp stem cells: An in vitro studySaudi Endodontic Journalmolars (adult) and characterized were purchased from AllCells (CA, USA). The complete (AllCells), with 50 ml of human mesenchymal stem cell stimulatory supplements (AllCells). SaveFull Paper
2018Goncalves, K;Hoban, M;Proctor, J;Hyzy, S;Adams, H;Gardner, J;Boitano, A;Cooke, M;Comparison of hematopoietic stem cell expansion methods reveals that aryl hydrocarbon receptor inhibition optimally expands CD90 engrafting cellsSSRN Electronic JournalHuman cord blood CD34+ cells or mobilized peripheral blood CD34+ cells (male or female) were purchased from AllCells. Cell number, purity, and viability was confirmed at time of thaw. Cell counts were obtained using TruCount Tubes (BD). Cell viabilitFull Paper
2018laura, ;Danilevsky, M;Li, Y;Reiss, F;Zhu, H;SystemT: Declarative Text Understanding for EnterpriseProceedings of the 2018 Conference of the North American Chapter of the Association for Computational Linguistics: Human Language Technologies, Volume 3 (Industry Papers)able in AQL, including dictionary matching (AssetClassSuffixes), core NLP operators such as Semantic Role Labeling (SRL) (Verbs, Arguments), and document structure operators such as table structure understanding (AllCells). Sec. 3 details the data moFull Paper
2018Abhyankar, V;The chemokine and cytokine responses of a keratinocyte, dendritic cell, and T-cell co-culture model treated with P. gingivalis hemagglutinin B (HagB)University of IowaBackground: P. gingivalis, a non-motile, rod-shaped, anaerobic, Gram-negative bacterium is one of the principal sources of periodontal disease. It possesses a number of potential SaveFull Paper
2018Sabhachandani, ;Development of microfluidic microanalysis systems for cancer therapeutic screeningNortheastern Universitycells were purchased from AllCells (#PB009-3F, AllCells, Alameda, CA). Cells were maintained in RPMI-1640 medium supplemented with 10% Fetal Bovine Serum and 1% Antibiotic-Antimycotic solution (Corning Cellgro, Manassas, VA). Primary human CD56+ NK cFull Paper
2018Bates, A;Matrix metalloproteinase and cytokine profiles from cell co-cultures and their role in oral inflammation and head and neck cancerUniversity of IowaHuman monocyte-derived immature myeloid dendritic cells (DC, AllCells, Alameda, CA) were positively identified by flow cytometry and contained DC of myeloid origin: 31.5% CD1c+/79.1% CD11c+ cells and 54.4% CD141+ /79.1% CD11c+ cells (Human myeloid DCFull Paper
2018Nada, M;Adoptive cancer immunotherapy with human Vγ2vδ2 T cellsUniversity of IowaEx vivo expansion of Vγ2Vδ2 T cells PBMC from random healthy adult donors were obtained by density centrifugation over Ficoll-Hypaque (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) of either random donor leukopaks (12 donors) (obtained from AllCeFull Paper
2018Twyman, RM;Wisden, W;Development, Molecular AspectsAdvance Molecular BiologyA fate map is a map of an egg or embryo which shows the fates of allcells A cell which will form gametes. Germ cells usually segregate from somatic cells early in animal development SaveFull Paper
2018Riepe, C;Zelin, E;Wyman, S;Nguyen, D;Liang, J;Frankino, P;Meacham, Z;Vu, J;Marson, A;Ingolia, N;Corn, J;Double Stranded DNA Breaks and Genome Editing Trigger Ribosome Remodeling and Translational ShutdownbioRxivMobilized Peripheral Blood CD34+ Stem/Progenitor Cells (AllCells) were cultured in StemSpan™ Serum Free Expansion Media II (STEMCELL Technologies), StemSpan™ StemSpan(tm) CC110 (STEMCELL Technologies), 1% Pen/StrepFull Paper
2018Mimitou, E;Cheng, A;Montalbano, A;Hao, S;Stoeckius, M;Legut, M;Roush, T;Herrera, A;Papalexi, E;Ouyang, Z;Satija, R;Sanjana, N;Koralov, S;Smibert, P;Expanding the CITE-seq tool-kit: Detection of proteins, transcriptomes, clonotypes and CRISPR perturbations with multiplexing, in a single assaybioRxivDMSO and aliquots of 1-1.5 million cells were thawed on the day of the experiment. Peripheral blood mononuclear cells (PBMCs) were obtained cryopreserved from AllCells (USA) and used immediately after thawing. NIH-3T3 (mouse) cells expressing non-tarFull Paper
2018Setty, M;Kiseliovas, V;Levine, J;Gayoso, A;Mazutis, L;Pe’er, D;Palantir characterizes cell fate continuities in human hematopoiesisbioRxivCryopreserved bone marrow stem/progenitor CD34+ cells from healthy donors were purchased from AllCells, LLC. (Cat. No. ABM022F) and stored in vapor phase nitrogen until use. Typical for scRNA-seq, a vial was removed from the storage and immediately tFull Paper
2018Shin, J;Wyman, S;Dewitt, M;Bray, N;Vu, J;Corn, J;Controlled cycling and quiescence enables homology directed repair in engraftment-enriched adult hematopoietic stem and progenitor cellsbioRxivCryopreserved wildtype human mobilized peripheral blood CD34+ HSPCs from multiple volunteer donors including male and female whose age ranged from 20-35 were purchased from Allcells, IncFull Paper
2018Wong, D;Immuno-Chemotherapeutic Platinum(IV) Prodrugs of Cisplatin as Multimodal Anticancer AgentsRethinking Platinum Anticancer Drug Design: Towards Targeted and Immuno-chemotherapeutic ApproachesPrior to activation, frozen PBMCs (AllCells LLC, USA) were thawed and washed twice with complete RPMI to remove freezing medium. 4.1 × 10 6 PBMCs were incubated in 5 mL of SaveFull Paper
2018Johnsen, KH;Performance Analysis and Optimization of the Equelle DSLThesisGradient() takes a CollOfScalar as argument which is defined on AllCells and returns a CollOfScalar which is defined on InteriorFaces. The Divergence() function is used to calculate the SaveFull Paper
2018Ganaie, SS;Essential Host Factors for Human Parvovirus B19 ReplicationThesisWe purchased CD34+ hematopoietic stem cells, which were isolated from bone marrow of a healthy human donor, from AllCells LLC (Alameda, CA) without any identification information on the cells, and, therefore, an institutional review board (IRB) revieFull Paper
2018Fink, EC;Investigating the Mechanism and Species-Specificity of Thalidomide DerivativesThesisControl cord-blood CD34+ cells were isolated as above. Adult bone marrow CD34+ cells were purchased as single-donor lots from AllCells (Alameda, CA). The number of replicates for each patient sample, vector, and treatment was limited by the number ofFull Paper
2018Ahmed, MA;Exploring the impact of hypoxia mimetic agents on multipotent stem cell biologyThesisFresh human bone marrow aspirate ABM001-1 ALLCELLS Gel Loading Buffer (0.05% bromophenol blue, 40% sucrose, 0.1 M EDTA, 0.5% SDS) G2526 Sigma-Aldrich Glycine 50046 SIGMA-ALDRICH Horse serum H 1270 Sigma-Aldrich GAP-43 Antibody NB300-143 Novus BiologiFull Paper
2018Sebastian Kettinger, N;Investigating the HIV Reservoir in Hematopoietic Stem and Progenitor CellsThesis(eBioscience), and 7-Aminoactinomycin D (7-AAD). Healthy donor BMMCs (AllCells) were stained with R-phycoerythrin-conjugated anti-CD133, R-phycoerythrin-Cy7-conjugated anti-CD34 (BD), and 7-AAD. Samples were analyzed on a BD FACSCanFull Paper
2018Al-Jumaily, RMK;Molecular cytology of hypoxic cancer and stem cells: an epigenetic approachThesis1 BMA-16 Human Bone Marrow, Male, Age-29 years Lonza, MD, USA 2 BMA-20 Human Bone Marrow, Female, Age-50 years AllCells, LLC, SA 3 BMA-25 Human Bone Marrow, Male, Age-27 years Lonza, MD, USAFull Paper
2018Becker, AH;Epigenetic and Gene Expression Changes Mediated by Histone H3 Methylation in Acute Myeloid LeukemiaThesis2.1.2 Bone marrow CD34+ cells Cryopreserved bone marrow CD34+ cells from normal donors were obtained from Allcells (Alameda, CA). 2.1.3 K562 cells Human K562 cells (ATCC CCL-243) were grown in RPMI 1640 GlutaMAX medium (Gibco, Thermo Fisher ScientifiFull Paper
2018Bartenstein, M;Symptomatic treatment of myelofibrosis by TGF-β1 signaling inhibitionThesisUniversity of Chicago. Cells were purified immune-magnetically from granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood of healthy donors purchased from AllCells, Inc. using a paramagnetically labeled mouse anti-human CD34 antiboFull Paper
2018Siriwon, N;Engineered CAR-T Cells for Treatment of Solid CancersThesisFrozen human PBMCs were obtained from AllCells (Alameda, CA). PBMCs were thawed in TCM and rested overnight. Before retroviral transduction, PBMCs were activated for 2 days by culturing with Dynabeads human T-activator CD3/CD28 (Thermo Fisher ScientiFull Paper
2018Seet, C;In Vitro Generation of Adaptive Immunity from Hematopoietic Stem CellsThesisdeliveries at UCLA. Bone marrow (BM) was obtained from healthy adult donors (ages 18-51) through discarded material from allogeneic BM donor harvests at UCLA or purchased from AllCells Inc. (Alameda, CA). G-CSF mobilized peripheral blood was obtainedFull Paper
2018Lin, HA;Development and Functional Evaluation of Injectable Cellulose-based Hydrogels as Nucleus Pulposus Replacements for Intervertebral Disc RepairThesis2.2.4. Cell-laden hydrogel fabrication and characterization 2.2.4.1. Primary cell isolation Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density gradient centrifugation as previously described (Gupta et al., 201Full Paper
2018Marvin, CM;Spatial and Temporal Delivery of Small Molecules from Long Wavelength Sensitive Phototherapeutcs Using Erythrocytes as Biological CarriersThesis525 nm and 660 nm LEDs were purchased from LED Supply. 725 nm and 780 nm LEDs were purchased from Techmark. Erythrocytes were purchased from Allcells. HRMS data was acquired on a Thermo Scientific electrospray linear trap quadrupole fourier transformFull Paper
2018Hu, C;Data-Driven Discovery of Proteomic Hallmarks in Acute Myeloid LeukemiaThesisThe samples were normalized to a concentration of 1x104 cells/µL and a whole cell lysate was prepared as described in publications [103, 36]. In addition to AML patient samples, each array includes the same ten cryopreserved normal bone marrow CD34+Full Paper
2018Sun, V;Engineering Solutions for Biomaterials: Self-Assembly and Surface-Modification of Polymers for Clinical ApplicationsThesisHuman peripheral blood mononuclear cells (PBMCs) were obtained from AllCells (Alameda, CA). Frozen PBMCs were thawed and cultured in TCM containing X-VIVO™ 15 serum-free medium (Lonza, Allendale NJ), 5% (vol/vol) GemCell human serum antibody AB (GemiFull Paper
2018Weng, H;Huang, H;Wu, H;Qin, X;Zhao, BS;Dong, L;Shi, H;Skibbe, J;Shen, C;Hu, C;Sheng, Y;Wang, Y;Wunderlich, M;Zhang, B;Dore, LC;Su, R;Deng, X;Ferchen, K;Li, C;Sun, M;Lu, Z;Jiang, X;Marcucci, G;Mulloy, JC;Yang, J;Qian, Z;Wei, M;He, C;Chen, J;METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and Promotes Leukemogenesis via mRNA m6A ModificationCell stem cell29290617were then co-transduced with different combinations of retroviruses or lentiviruses as indicated through two rounds of “spinoculation”. Cells were then plated into ColonyGEL methylcellulose medium (ReachBio, Seattle, WA) supplied with 10 ng/ml of murFull Paper
2018Probst, K;Stermann, J;von Bomhard, I;Etich, J;Pitzler, L;Niehoff, A;Bluhm, B;Xu, HC;Lang, PA;Chmielewski, M;Abken, H;Blissenbach, B;Machova, A;Papadopoulou, N;Brachvogel, B;Depletion of Collagen IX Alpha1 Impairs Myeloid Cell FunctionStem cells (Dayton, Ohio)30063808Flushed bone marrow samples from 2 months old mice were resuspended in Iscove's Modified Dulbecco's Medium containing 10% FCS and used for the colony forming unit assay (ColonyGEL 1203, Reachbio, Seattle)Full Paper
2018Li, G;Boucher, JC;Kotani, H;Park, K;Zhang, Y;Shrestha, B;Wang, X;Guan, L;Beatty, N;Abate-Daga, D;Davila, ML;4-1BB enhancement of CAR T function requires NF-κB and TRAFsJCI insight30232281Human peripheral blood mononuclear cells from healthy donors were purchased from ReachBio. Human T cell complete medium consists of RPMI1640 medium, 10% FBS, 2 mM L- SaveFull Paper
2018Kong, J;Han, L;Su, H;Hu, Y;Huang, X;Lou, Y;Riligustilide Attenuated Renal Injury by the Blockade of ReninCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology30308499Mouse anti-CBP monoclonal antibody SC-7300; rabbit anti-CREB polyclonal antibody SC-186 were commercially purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA;). CY5 was obtained from ReachBio WA, USA (#111-165-003), and Alexa Fluor® 488 wasFull Paper
2018Psatha, N;Reik, A;Phelps, S;Zhou, Y;Dalas, D;Yannaki, E;Levasseur, DN;Urnov, FD;Holmes, MC;Papayannopoulou, T;Disruption of the BCL11A Erythroid Enhancer Reactivates Fetal Hemoglobin in Erythroid Cells of Patients with β-Thalassemia MajorMolecular therapy. Methods & clinical development30182035anulocyte macrophage [GM] and burst-forming unit-erythroid [BFU-E]), cells were plated at a density of 750-2,000 cells/mL in semi-solid methylcellulose-based medium containing cytokines, ColonyGEL (ReachBio). Zinc Finger Nucleases ZFN pairs were expFull Paper
2018Li, C;Psatha, N;Gil, S;Wang, H;Papayannopoulou, T;Lieber, A;HDAd5/35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem CellsMolecular therapy. Methods & clinical development30038942ng/mL SCF, 3 IU/mL EPO, 10 μg/mL insulin, 330 μg/mL transferrin, 2 U/mL heparin, and 1 μg/mL DOX for 6 days. CFU Assay CFU assays with transduced CD34+ cells were performed using ColonyGEL (Reachbio, Seattle, WA, USA) with human complete medium accoFull Paper
2018Wang, H;Richter, M;Psatha, N;Li, C;Kim, J;Liu, J;Ehrhardt, A;Nilsson, SK;Cao, B;Palmer, D;Ng, P;Izsvák, Z;Haworth, KG;Kiem, HP;Papayannopoulou, T;Lieber, A;A Combined In Vivo HSC Transduction/Selection Approach Results in Efficient and Stable Gene Expression in Peripheral Blood Cells in MiceMolecular therapy. Methods & clinical development29255741Biotec, San Diego, CA, USA) was used in accordance with the manufacturer’s instructions. CFU Assay 1,500 lineage−, GFP+ cells were plated in triplicates in ColonyGEL 1202 mouse complete medium (ReachBio, Seattle, WA, USA) and incubated for 12 days aFull Paper
2017Lim, JS;Ibaseta, A;Fischer, MM;Cancilla, B;O'Young, G;Cristea, S;Luca, VC;Yang, D;Jahchan, NS;Hamard, C;Antoine, M;Wislez, M;Kong, C;Cain, J;Liu, YW;Kapoun, AM;Garcia, KC;Hoey, T;Murriel, CL;Sage, J;Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancerNature28489825... Luminex assay for midkine protein in human plasma Human plasma samples from cancer-free normal donors were purchased from BioreclamationIVT. SCLC donor plasma was sourced from Conversant Biologics (Conversant Bio). The samples were collected, pFull Paper
2017Alley, EW;Lopez, J;Santoro, A;Morosky, A;Saraf, S;Piperdi, B;van Brummelen, E;Clinical safety and activity of pembrolizumab in patients with malignant pleural mesothelioma (KEYNOTE-028): preliminary results from a non-randomised, open-label, phase 1b trialLancet Oncol.28291584PD-L1 expression was assessed in archival or new tumour samples by a central laboratory (QualTek Molecular Laboratories, Goleta, CA, USA) with a prototype assay and the 22C3 antibody (Merck, Kenilworth, NJ, USA).22, 23 PD-L1 positivity was defined byFull Paper
2017Plimack, ER;Bellmunt, J;Gupta, S;Berger, R;Chow, LQ;Juco, J;Lunceford, J;Saraf, S;Perini, RF;O'Donnell, PH;Safety and activity of pembrolizumab in patients with locally advanced or metastatic urothelial cancer (KEYNOTE-012): a non-randomised, open-label, phase 1b studyLancet Oncol.28081914Initially, we used a prototype PD-L1 immunohistochemical assay to establish the eligibility of patients in KEYNOTE-012, which was done at a laboratory site at QualTek (Goleta, CA, USA) accredited by the College of American Pathologists and Clinical LFull Paper
2017Frenel, JS;Le Tourneau, C;O'Neil, B;Ott, PA;Piha-Paul, SA;Gomez-Roca, C;van Brummelen, EMJ;Rugo, HS;Thomas, S;Saraf, S;Rangwala, R;Varga, A;Safety and Efficacy of Pembrolizumab in Advanced, Programmed Death Ligand 1-Positive Cervical Cancer: Results From the Phase Ib KEYNOTE-028 TrialJ. Clin. Oncol.29095678tumor sample or newly obtained core or excisional biopsy sample, was defined as membranous staining on $ 1% modified proportion score or interface pattern as assessed using a laboratory-developed prototype immunohistochemistry assay (QualTek MoleculaFull Paper
2017Hsu, C;Lee, SH;Ejadi, S;Even, C;Cohen, RB;Le Tourneau, C;Mehnert, JM;Algazi, A;van Brummelen, EMJ;Saraf, S;Thanigaimani, P;Cheng, JD;Hansen, AR;Safety and Antitumor Activity of Pembrolizumab in Patients With Programmed Death-Ligand 1-Positive Nasopharyngeal Carcinoma: Results of the KEYNOTE-028 StudyJ. Clin. Oncol.28837405Tumor PD-L1 positivity was established at a central laboratory at baseline on an archived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy sample using a laboratory-developed prototype immunohistochemical assay (QualTek MolecFull Paper
2017Ott, PA;Elez, E;Hiret, S;Kim, DW;Morosky, A;Saraf, S;Piperdi, B;Mehnert, JM;Pembrolizumab in Patients With Extensive-Stage Small-Cell Lung Cancer: Results From the Phase Ib KEYNOTE-028 StudyJ. Clin. Oncol.28813164Tumor PD-L1 expression was required to be eligible for treatment in the study; it was evaluated in archival or fresh tumor samples by a central laboratory (QualTek Molecular Laboratories, Goleta, CA) by using a prototype assay and the 22C3 antibody (Full Paper
2017Chen, R;Zinzani, PL;Fanale, MA;Armand, P;Johnson, NA;Brice, P;Radford, J;Ribrag, V;Molin, D;Vassilakopoulos, TP;Tomita, A;von Tresckow, B;Shipp, MA;Zhang, Y;Ricart, AD;Balakumaran, A;Moskowitz, CH;, ;Phase II Study of the Efficacy and Safety of Pembrolizumab for Relapsed/Refractory Classic Hodgkin LymphomaJ. Clin. Oncol.28441111PD-L1 expression was determined as previously described, 16 using fresh or archival formalin-fixed, paraffin-embedded pretreatment tissue sectioned at 4 to 5 microns, with a proprietary immunohistochemical assay developed at QualTek Molecular LaboratFull Paper
2017Colombo, I;Lien, S;Yang, C;Clouthier, D;Bonilla, L;Cyriac, S;Ethier, J;Lee, Y;Kanjanapan, Y;Mandilaras, V;Dhani, N;Butler, M;Oza, A;Quintos, J;Chow, H;Pugh, T;Ohashi, P;Siu, L;Lheureux, S;Immunologic and genomic characterization of high grade serous ovarian cancer (HGSOC) in patients (pts) treated with pembrolizumab (Pembro) in the phase II INSPIRE trial.JCOMean Tumor Proportion Score of PD-L1 by IHC (Qualtek) was 6.4% (range 0-30%). Grade 3/4 adverse events possibly related to Pembro were observed in 4/18 (22%) pts; none was fatal and the most common were fatigue and hyponatremiaFull Paper
2017Toy, W;Weir, H;Razavi, P;Lawson, M;Goeppert, AU;Mazzola, AM;Smith, A;Wilson, J;Morrow, C;Wong, WL;De Stanchina, E;Carlson, KE;Martin, TS;Uddin, S;Li, Z;Fanning, S;Katzenellenbogen, JA;Greene, G;Baselga, J;Chandarlapaty, S;Activating ESR1 Mutations Differentially Affect the Efficacy of ER AntagonistsCancer Discov27986707... n). PATIENT-DERIVED XENOGRAFTS (PDX) CTC-714 PDX model was derived from patient circulating tumor cells (CTC) cultures, consented according to the Human Biological Samples Policy and purchased from Conversant Biologics. The CTCs were obtained fFull Paper
2017Li, J;Stagg, NJ;Johnston, J;Harris, MJ;Menzies, SA;DiCara, D;Clark, V;Hristopoulos, M;Cook, R;Slaga, D;Nakamura, R;McCarty, L;Sukumaran, S;Luis, E;Ye, Z;Wu, TD;Sumiyoshi, T;Danilenko, D;Lee, GY;Totpal, K;Ellerman, D;Hötzel, I;James, JR;Junttila, TT;Membrane-Proximal Epitope Facilitates Efficient T Cell Synapse Formation by Anti-FcRH5/CD3 and Is a Requirement for Myeloma Cell KillingCancer Cell28262555... ec). CD4+ or CD8+ cells were used as effectors in a 3:1 effector:target ratio. In Vitro Cytotoxicity Assay: Human Plasma Cells and Primary MM Samples Human BMMCs from MM patients were procured from Conversant Bio. Human bone marrow aspirates ofFull Paper
2017Le Pichon, CE;Meilandt, WJ;Dominguez, S;Solanoy, H;Lin, H;Ngu, H;Gogineni, A;Sengupta Ghosh, A;Jiang, Z;Lee, SH;Maloney, J;Gandham, VD;Pozniak, CD;Wang, B;Lee, S;Siu, M;Patel, S;Modrusan, Z;Liu, X;Rudhard, Y;Baca, M;Gustafson, A;Kaminker, J;Carano, RAD;Huang, EJ;Foreman, O;Weimer, R;Scearce-Levie, K;Lewcock, JW;Loss of dual leucine zipper kinase signaling is protective in animal models of neurodegenerative diseaseSci Transl Med28814543AD (early-stage AD) or confirmed AD along with age-matched controls from Folio Biosciences or Banner Sun Health Research Institute. All human tissues were collected with informed consents and institutional review board approvals.Full Paper
2017Pearlman, R;Frankel, WL;Swanson, B;Zhao, W;Yilmaz, A;Miller, K;Bacher, J;Bigley, C;Nelsen, L;Goodfellow, PJ;Goldberg, RM;Paskett, E;Shields, PG;Freudenheim, JL;Stanich, PP;Lattimer, I;Arnold, M;Liyanarachchi, S;Kalady, M;Heald, B;Greenwood, C;Paquette, I;Prues, M;Draper, DJ;Lindeman, C;Kuebler, JP;Reynolds, K;Brell, JM;Shaper, AA;Mahesh, S;Buie, N;Weeman, K;Shine, K;Haut, M;Edwards, J;Bastola, S;Wickham, K;Khanduja, KS;Zacks, R;Pritchard, CC;Shirts, BH;Jacobson, A;Allen, B;de la Chapelle, A;Hampel, H;, ;Prevalence and Spectrum of Germline Cancer Susceptibility Gene Mutations Among Patients With Early-Onset Colorectal CancerJAMA Oncol27978560Myriad Genetics Inc donated the next-generation sequencing testing for mismatch repair–proficient patients with CRC diagnosed younger than 50 years for this study. Dr Kalady discloses honorarium from Helomics and Transenterix and is a member of the sFull Paper
2017Ott, PA;Piha-Paul, SA;Munster, P;Pishvaian, MJ;van Brummelen, EMJ;Cohen, RB;Gomez-Roca, C;Ejadi, S;Stein, M;Chan, E;Simonelli, M;Morosky, A;Saraf, S;Emancipator, K;Koshiji, M;Bennouna, J;Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with recurrent carcinoma of the anal canalAnn. Oncol.28453692mbedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek Molecular Laboratories, Goleta, CA) [15] uFull Paper
2017Hui, R;Garon, EB;Goldman, JW;Leighl, NB;Hellmann, MD;Patnaik, A;Gandhi, L;Eder, JP;Ahn, MJ;Horn, L;Felip, E;Carcereny, E;Rangwala, R;Lubiniecki, GM;Zhang, J;Emancipator, K;Roach, C;Rizvi, NA;Pembrolizumab as first-line therapy for patients with PD-L1-positive advanced non-small cell lung cancer: a phase 1 trialAnn. Oncol.28168303As previously described [5], PD-L1 expression for enrollment was assessed with a prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA) and the relationship between PD-L1 expression and efficacy was investigated using a cliFull Paper
2017Potts, AH;Vakulskas, CA;Pannuri, A;Yakhnin, H;Babitzke, P;Romeo, T;Global role of the bacterial post-transcriptional regulator CsrA revealed by integrated transcriptomicsNat Commun29150605;... nufacturer’s instructions with one modification. Adaptors and reverse transcription primers were diluted 1 in 5 in water before use. Libraries were sequenced using HiSeq 2500 50SE (Illumina) by the HudsonAlpha Genomic Services Laboratory. SequeFull Paper
2017Christopher, MA;Myrick, DA;Barwick, BG;Engstrom, AK;Porter-Stransky, KA;Boss, JM;Weinshenker, D;Levey, AI;Katz, DJ;LSD1 protects against hippocampal and cortical neurodegenerationNat Commun28993646... s possible, then RNA was precipitated with isopropanol. Pellets were then washed with 75% ethanol and resuspended in 50 µL deionized water. RNA library preparation and sequencing were performed by HudsonAlpha Genomic Services Lab. RNA was Poly(A)Full Paper
2017Ramaker, RC;Savic, D;Hardigan, AA;Newberry, K;Cooper, GM;Myers, RM;Cooper, SJ;A genome-wide interactome of DNA-associated proteins in the human liverGenome Res.29021291HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for BiFull Paper
2017Cubeñas-Potts, C;Rowley, MJ;Lyu, X;Li, G;Lei, EP;Corces, VG;Different enhancer classes in Drosophila bind distinct architectural proteins and mediate unique chromatin interactions and 3D architectureNucleic Acids Res.27899590New ChIP experiments were performed in Kc167 cells for GAF, H3, H3K27me3, Ibf1, Ibf2, Pita, Nup98 and ZIPIC as described previously with minor modifications (28). More precisely, antibody-bound protein complexes were isolated with Protein A or ProteiFull Paper
2017Edwardson, CF;Hollibaugh, JT;Metatranscriptomic analysis of prokaryotic communities active in sulfur and arsenic cycling in Mono Lake, California, USAISME J28548659synthesis kits. Libraries (~225 bp insert) were prepared using Illumina TruSeq technology. Samples were pooled and run on one lane of 150 × 2 Illumina HiSeq 2500 Rapid Run at HudsonAlpha Genomic Services. Reads haveFull Paper
2017Yeh, TC;O'Connor, G;Petteruti, P;Dulak, A;Hattersley, M;Barrett, JC;Chen, H;Identification of CCR2 and CD180 as Robust Pharmacodynamic Tumor and Blood Biomarkers for Clinical Use with BRD4/BET InhibitorsClin. Cancer Res.28073847Whole blood was collected in 10 mL EDTA vaccutainers (Becton Dickinson) from two control healthy volunteers and 11 patients with cancer with solid tumor or hematologic cancers through Conversant Bio. Compound incubation and sample processing was carrFull Paper
2017Kirtane, AR;Sadhukha, T;Kim, H;Khanna, V;Koniar, B;Panyam, J;Fibrinolytic Enzyme Cotherapy Improves Tumor Perfusion and Therapeutic Efficacy of Anticancer NanomedicineCancer Res.28108516... anticancer nanomedicine in syngeneic mouse melanoma and xenograft lung cancer models. Go to: MATERIALS AND METHODS MATERIALS Tissue microarrays of various human tumor biopsies were obtained from Folio Biosciences (Ohio, USA). Anti-human fibrFull Paper
2017Jung, YH;Sauria, MEG;Lyu, X;Cheema, MS;Ausio, J;Taylor, J;Corces, VG;Chromatin States in Mouse Sperm Correlate with Embryonic and Adult Regulatory LandscapesCell Rep28178516We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology, and specially Drs. Braden Boone, Angela Jones and Terri Pointer, for their help in performing Illumina sequencing of samples. This work was supported by UFull Paper
2017O'Daniel, JM;McLaughlin, HM;Amendola, LM;Bale, SJ;Berg, JS;Bick, D;Bowling, KM;Chao, EC;Chung, WK;Conlin, LK;Cooper, GM;Das, S;Deignan, JL;Dorschner, MO;Evans, JP;Ghazani, AA;Goddard, KA;Gornick, M;Farwell Hagman, KD;Hambuch, T;Hegde, M;Hindorff, LA;Holm, IA;Jarvik, GP;Knight Johnson, A;Mighion, L;Morra, M;Plon, SE;Punj, S;Richards, CS;Santani, A;Shirts, BH;Spinner, NB;Tang, S;Weck, KE;Wolf, SM;Yang, Y;Rehm, HL;A survey of current practices for genomic sequencing test interpretation and reporting processes in US laboratoriesGenet. Med.27811861Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.++Laboratory for Molecular Medicine, Partners Healthcare Personalized Medicine, Cambridge, Massachusetts, USA.++Division of Medical Genetics, UniverFull Paper
2017Rydzak, T;Garcia, D;Stevenson, DM;Sladek, M;Klingeman, DM;Holwerda, EK;Amador-Noguez, D;Brown, SD;Guss, AM;Deletion of Type I glutamine synthetase deregulates nitrogen metabolism and increases ethanol production in Clostridium thermocellumMetab. Eng.28400329; diluted. Sequencing was completed using a SR50 sequencing protocol and V4 chemistry on an Illumina HiSeq. 2500 platform (HudsonAlpha Genomic Services Laboratory; Huntsville, AL). 2.7. RNA-Seq analysis. Raw readsFull Paper
2017Tigue, NJ;Bamber, L;Andrews, J;Ireland, S;Hair, J;Carter, E;Sridharan, S;Jovanović, J;Rees, DG;Springall, JS;Solier, E;Li, YM;Chodorge, M;Perez-Martinez, D;Higazi, DR;Oberst, M;Kennedy, M;Black, CM;Yan, L;Schwickart, M;Maguire, S;Cann, JA;de Haan, L;Young, LL;Vaughan, T;Wilkinson, RW;Stewart, R;MEDI1873, a potent, stabilized hexameric agonist of human GITR with regulatory T-cell targeting potentialOncoimmunology28405505... xP3 Tissue samples from 76 cancer patients, with NSCLC, colorectal cancer (CRC), bladder cancer, or squamous cell cancer of the head and neck (SCCHN), were obtained from commercial sources (Asterand, ConversantBio, ProteoGenex, National Disease RFull Paper
2017Liu, Z;Zhang, C;Skamagki, M;Khodadadi-Jamayran, A;Zhang, W;Kong, D;Chang, CW;Feng, J;Han, X;Townes, TM;Li, H;Kim, K;Zhao, R;Elevated p53 Activities Restrict Differentiation Potential of MicroRNA-Deficient Pluripotent Stem CellsStem Cell Reports29141234Total RNA was extracted from Dgcr8−/−-302 cells and submitted to the Genomic Services Lab at the HudsonAlpha Institute. The library was constructed by the standard miRNA library construction protocol (Illumina) and 15 million, 50 bp single-end readsFull Paper
2017Bowling, KM;Thompson, ML;Amaral, MD;Finnila, CR;Hiatt, SM;Engel, KL;Cochran, JN;Brothers, KB;East, KM;Gray, DE;Kelley, WV;Lamb, NE;Lose, EJ;Rich, CA;Simmons, S;Whittle, JS;Weaver, BT;Nesmith, AS;Myers, RM;Barsh, GS;Bebin, EM;Cooper, GM;Genomic diagnosis for children with intellectual disability and/or developmental delayGenome Med28554332Methods" (see Additional file 1). Whole-exome and whole-genome sequencing. Blood samples were sent for sequencing at the HudsonAlpha Genomic Services Laboratory (http://gsl.hudsonalpha.org). Genomic DNA was isolatedFull Paper
2017Harden, ME;Prasad, N;Griffiths, A;Munger, K;Modulation of microRNA-mRNA Target Pairs by Human Papillomavirus 16 OncoproteinsMBio28049151;... million 50-bp PE reads) was performed with the Illumina MiSeq Sequencing System (Illumina). miR-seq data analysis. Postprocessing of the miR-seq reads from each sample was performed according to the HudsonAlpha Genomic Services Laboratory (GSL)Full Paper
2017Weiss, G;Schlegel, A;Kottwitz, D;König, T;Tetzner, R;Validation of the SHOX2/PTGER4 DNA Methylation Marker Panel for Plasma-Based Discrimination between Patients with Malignant and Nonmalignant Lung DiseaseJ Thorac Oncol27544059The patient samples were either material collected over the course of several months by commercial partners (ProteoGenex Inc., Sofia Bio LLC) or leftover material from previously conducted clinical trials in colon cancer.21, 22 More specifically, patFull Paper
2017Steffen, MM;Davis, TW;McKay, RML;Bullerjahn, GS;Krausfeldt, LE;Stough, JMA;Neitzey, ML;Gilbert, NE;Boyer, GL;Johengen, TH;Gossiaux, DC;Burtner, AM;Palladino, D;Rowe, MD;Dick, GJ;Meyer, KA;Levy, S;Boone, BE;Stumpf, RP;Wynne, TT;Zimba, PV;Gutierrez, D;Wilhelm, SW;Ecophysiological Examination of the Lake Erie Microcystis Bloom in 2014: Linkages between Biology and the Water Supply Shutdown of Toledo, OHEnviron. Sci. Technol.28535339RNA was stored at −80 °C until sent to HudsonAlpha Institute for Biotechnology (Huntsville, AL) for sequencing. Total RNA concentrations and quality were assessed fluorometrically via RiboGreen (Life Technologies, Carlsbad, CA) followed by integrityFull Paper
2017Park, KC;Lee, M;Jeon, Y;Jeon, R;Baek, SH;Lee, H;Kim, KI;Skin-Specific Deletion of Mis18α Impedes Proliferation and Stratification of Epidermal KeratinocytesJ. Invest. Dermatol.27670610Materials and Methods. In situ hybridization. All procedures of in situ hybridization, except probe design and cloning, were performed by Phylogeny Inc. (Columbus, OH). Briefly, the whole bodies of C57BL/6 mice were frozen and cut into 10-μm sectionsFull Paper
2017Blanchet, P;Bebin, M;Bruet, S;Cooper, GM;Thompson, ML;Duban-Bedu, B;Gerard, B;Piton, A;Suckno, S;Deshpande, C;Clowes, V;Vogt, J;Turnpenny, P;Williamson, MP;Alembik, Y;, ;, ;Glasgow, E;McNeill, A;MYT1L mutations cause intellectual disability and variable obesity by dysregulating gene expression and development of the neuroendocrine hypothalamusPLoS Genet.28859103Département de Génétique Médicale, Hôpital Arnaud de Villeneuve, Montpellier Cedex 5, France.++Department of Neurology, University of Alabama at Birmingham, Birmingham, AL, United States of America.++Service de génétique médicale, Hôpitaux UniversitaFull Paper
2017Mojiri, A;Stoletov, K;Carrillo, MA;Willetts, L;Jain, S;Godbout, R;Jurasz, P;Sergi, CM;Eisenstat, DD;Lewis, JD;Jahroudi, N;Functional assessment of von Willebrand factor expression by cancer cells of non-endothelial originOncotarget28035064Immunofluorescent staining of cultured cells were performed as previously described [34]. Immunofluorescent staining of slides containing sectioned paraffin imbedded biopsies of normal human brain and glioma (Dr. D. Eisenstat from the Brain Tumor TisFull Paper
2017McDaniel, JM;Varley, KE;Gertz, J;Savic, DS;Roberts, BS;Bailey, SK;Shevde, LA;Ramaker, RC;Lasseigne, BN;Kirby, MK;Newberry, KM;Partridge, EC;Jones, AL;Boone, B;Levy, SE;Oliver, PG;Sexton, KC;Grizzle, WE;Forero, A;Buchsbaum, DJ;Cooper, SJ;Myers, RM;Genomic regulation of invasion by STAT3 in triple negative breast cancerOncotarget28030809... ell viability and invasion assays. All ChIP-seq and RNA-seq datasets have been deposited in GEO (accession number: GSE85579). SUPPLEMENTARY MATERIALS FIGURES AND TABLES We thank the members of the HudsonAlpha Genomic Services Laboratory for prFull Paper
2017Ranganathan, P;Ngankeu, A;Zitzer, NC;Leoncini, P;Yu, X;Casadei, L;Challagundla, K;Reichenbach, DK;Garman, S;Ruppert, AS;Volinia, S;Hofstetter, J;Efebera, YA;Devine, SM;Blazar, BR;Fabbri, M;Garzon, R;Serum miR-29a Is Upregulated in Acute Graft-versus-Host Disease and Activates Dendritic Cells through TLR BindingJ. Immunol.28159900... Go to: ACKNOWLEDGMENTS We thank Carlo M Croce at The Ohio State University for providing us with miR29a KO mice. We thank Jordan Smith for assisting in collection and processing of blood samples. Phylogeny Inc., Columbus, Ohio, performed LNA-Full Paper
2017Xu-Monette, ZY;Zhang, M;Li, J;Young, KH;PD-1/PD-L1 Blockade: Have We Found the Key to Unleash the Antitumor Immune Response?Front Immunol2925545899.5%; 75.6% of patients had a response for ≥6 months Clinical activity was seen across all PD-L1 groups defined by PD-L1 intensity score, tumor-membrane staining score, and histiocyte score (QualTek IHC assay); 90.4% of patients had an intensityFull Paper
2017Zeng, Q;Tian, X;Wang, L;Genetic adaptation of microbial populations present in high-intensity catfish production systems with therapeutic oxytetracycline treatmentSci Rep29235508;... DNA (~1000 ng/sample) extracted from every sediment sample collected from the treated or the control system, were pooled for sequencing. The library construction and sequencing were conducted at the HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2017Oliveira, ERA;Póvoa, TF;Nuovo, GJ;Allonso, D;Salomão, NG;Basílio-de-Oliveira, CA;Geraldo, LHM;Fonseca, CG;Lima, FRS;Mohana-Borges, R;Paes, MV;Dengue fatal cases present virus-specific HMGB1 response in peripheral organsSci Rep29167501... iro, Brazil 2Instituto de Criminalística, Tocantins, Brazil 30000 0001 2285 7943grid.261331.4Ohio State University Comprehensive Cancer Center, Columbus, Ohio, United States of America 4grid.430298.4Phylogeny Inc, Powell, Ohio, United States of AFull Paper
2017Zhang, Y;Saavedra, E;Tang, R;Gu, Y;Lappin, P;Trajkovic, D;Liu, SH;Smeal, T;Fantin, V;De Botton, S;Legrand, O;Delhommeau, F;Pernasetti, F;Louache, F;Targeting primary acute myeloid leukemia with a new CXCR4 antagonist IgG1 antibody (PF-06747143)Sci Rep28779088... ratio and 1:50 ratios, respectively (n = 4/group). ToxiLight bioluminescent cytotoxicity assay (Lonza) was used to detect cell lysis. The AML patient primary bone marrow sample was obtained from Conversant Bio (sample ID #110032873). Mice NSG mFull Paper
2017Aumiller, V;Strobel, B;Romeike, M;Schuler, M;Stierstorfer, BE;Kreuz, S;Comparative analysis of lysyl oxidase (like) family members in pulmonary fibrosisSci Rep28273952Normal and fibrotic lung tissue samples from the BLM-induced mouse model were prepared and Masson trichrome stained as described previously27. Human IPF and healthy lung tissue was purchased at Folio bioscience (Columbus, Ohio, USA) following the guiFull Paper
2017Jane, EP;Premkumar, DR;Sutera, PA;Cavaleri, JM;Pollack, IF;Survivin inhibitor YM155 induces mitochondrial dysfunction, autophagy, DNA damage and apoptosis in Bcl-xL silenced glioma cell linesMol. Carcinog.27805285collected at the time of tumor resection. We also obtained primary GBM cells from Conversant Biologics (Huntsville, AL), which were cultured in DMEM/F12 (Invitrogen, Carlsbad, CA). GIBCO® Human Astrocytes, normal human cellsFull Paper
2017Appleby, TC;Greenstein, AE;Hung, M;Liclican, A;Velasquez, M;Villaseñor, AG;Wang, R;Wong, MH;Liu, X;Papalia, GA;Schultz, BE;Sakowicz, R;Smith, V;Kwon, HJ;Biochemical characterization and structure determination of a potent, selective antibody inhibitor of human MMP9J. Biol. Chem.28235803Ulcerative colitis tissue was obtained after surgical colectomy (Folio Biosciences). Specimens were selected based on disease severity scored by a pathologist on matched FFPE tissues. Frozen fragments of ~50 mg were lysed in 10 mM Hepes pH 7.5, 150 mFull Paper
2017Cho, JH;Sorensen, SF;Choi, YL;Feng, Y;Kim, TE;Choi, H;Georgsen, JB;Dolled-Filhart, M;Emancipator, K;Meldgaard, P;Sun, JM;Kim, HK;Choi, YS;Shim, YM;Zhou, W;Hager, H;Kim, J;Programmed Death Ligand 1 Expression in Paired Non-Small Cell Lung Cancer Tumor SamplesClin Lung Cancer28669849charged slides. The TechMate 500 instrument (QualTek, Goleta, CA) was used with the 22C3 antibody for automated staining. A membrane staining. All of the PD-L1 IHC assays were done at QualTek, as the central laboratory. PDFull Paper
2017Sundahl, N;De Wolf, K;Rottey, S;Decaestecker, K;De Maeseneer, D;Meireson, A;Goetghebeur, E;Fonteyne, V;Verbeke, S;De Visschere, P;Reynders, D;Van Gele, M;Brochez, L;Ost, P;A phase I/II trial of fixed-dose stereotactic body radiotherapy with sequential or concurrent pembrolizumab in metastatic urothelial carcinoma: evaluation of safety and clinical and immunologic responseJ Transl Med28662677Exploratory endpoints. Response rate according to expression of PD-L1. PD-L1 expression will be determined by immunohistochemistry in QualTek Molecular Laboratories on an archival or newly obtained tissue sample of the tumor or metastatic lesionFull Paper
2017Lagatie, O;Van Dorst, B;Stuyver, LJ;Identification of three immunodominant motifs with atypical isotype profile scattered over the Onchocerca volvulus proteomePLoS Negl Trop Dis28125577For determination of specificity of the peptide ELISA’s, different sets of plasma samples were obtained from Discovery Life Sciences, Inc. (Los Osos, USA), Bioreclamation IVT (Baltimore, USA), FR3 or Mayo Clinic (Jacksonville, USA). Written informedFull Paper
2017Pellegrini, KL;Patil, D;Douglas, KJS;Lee, G;Wehrmeyer, K;Torlak, M;Clark, J;Cooper, CS;Moreno, CS;Sanda, MG;Detection of prostate cancer-specific transcripts in extracellular vesicles isolated from post-DRE urineProstate28419548; targeted in this analysis). The libraries were sequenced at the HudsonAlpha Genomic Services Lab to produce 150 bases of paired‐end reads with an average of over 300 000 reads mapped per sample. The 150 base sequenceFull Paper
2017Wilson, CM;Klingeman, DM;Schlachter, C;Syed, MH;Wu, CW;Guss, AM;Brown, SD;LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313Appl. Environ. Microbiol.28003194;... Samples were pooled into three groups to make a stock of 20 nM in 72 μl. A 40-μl aliquot of the three pooled libraries was sequenced using an SR50 sequencing run on an Illumina HiSeq 2500 platform (HudsonAlpha Genomic Services Laboratory, HuntsvFull Paper
2017Zhang, Y;Song, J;Day, K;Absher, D;dCATCH-Seq: improved sequencing of large continuous genomic targets with double-hybridizationBMC Genomics29061109HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA. dabsher@hudsonaFull Paper
2017Matsunaga, Y;Hwang, H;Franke, B;Williams, R;Penley, M;Qadota, H;Yi, H;Morran, LT;Lu, H;Mayans, O;Benian, GM;Twitchin kinase inhibits muscle activityMol. Biol. Cell28428253Genomic DNA from unc-22(sf21) was prepared and provided to the Emory Integrated Genomics Core (EIGC) for Illumina sequencing. The Genomic Services Laboratory at the HudsonAlpha Institute for Biotechnology performed quality control and constructed staFull Paper
2017Liu, Z;Zhang, C;Khodadadi-Jamayran, A;Dang, L;Han, X;Kim, K;Li, H;Zhao, R;Canonical microRNAs Enable Differentiation, Protect Against DNA Damage, and Promote Cholesterol Biosynthesis in Neural Stem CellsStem Cells Dev.27762676For mRNA-seq and small RNA-seq analyses, total RNA samples prepared from Dgcr8+/− and Dgcr8−/− NSCs were submitted to the Genomic Services Laboratory at HudsonAlpha Institute (Huntsville, AL) for library construction and sequencing. For mRNA-seq analFull Paper
2017Aycock, KI;Campbell, RL;Lynch, FC;Manning, KB;Craven, BA;Computational predictions of the embolus-trapping performance of an IVC filter in patient-specific and idealized IVC geometriesBiomech Model Mechanobiol28656515Full Paper
2017George, AF;Rahman, KM;Camp, ME;Prasad, N;Bartol, FF;Bagnell, CA;Defining age- and lactocrine-sensitive elements of the neonatal porcine uterine microRNA-mRNA interactomeBiol. Reprod.28203709Department of Animal Sciences, Endocrinology, and Animal Biosciences Program, Rutgers University, New Brunswick, New Jersey, USA.++Department of Animal Sciences, Endocrinology, and Animal Biosciences Program, Rutgers University, New Brunswick, New JeFull Paper
2017Kirby, MK;Ramaker, RC;Roberts, BS;Lasseigne, BN;Gunther, DS;Burwell, TC;Davis, NS;Gulzar, ZG;Absher, DM;Cooper, SJ;Brooks, JD;Myers, RM;Genome-wide DNA methylation measurements in prostate tissues uncovers novel prostate cancer diagnostic biomarkers and transcription factor binding patternsBMC Cancer28412973;... ng characteristic RRBS Reduced representation bisulfite sequencing TCGA The cancer genome atlas TF Transcription factor TMPRSS2 Transmembrane protease, serine 2 Acknowledgements We thank the HudsonAlpha Genomic Services Lab for providinFull Paper
2017Costa-Paiva, EM;Whelan, NV;Waits, DS;Santos, SR;Schrago, CG;Halanych, KM;Discovery and evolution of novel hemerythrin genes in annelid wormsBMC Evol. Biol.28330441Despite extensive study on hemoglobins and hemocyanins, little is known about hemerythrin (Hr) evolutionary history. Four subgroups of Hrs have been documented, including: circulating Hr (cHr), myohemerythrin (myoHr), ovohemerythrin (ovoHr), and neurFull Paper
2017Pizzi, M;Inghirami, G;Patient-derived tumor xenografts of lymphoproliferative disorders: are they surrogates for the human disease?Curr. Opin. Hematol.28594662Patient-derived xenograft models WuXi AppTec http://www.urolead.com Xenografts Conversant Bio http://www.conversantbio.com/pricing Xenograft models ONCOdesign http://www.oncodesign. com/en/contact-us Humanized mice HuMurine http://humurine.comFull Paper
2017Zhang, D;Thongda, W;Li, C;Zhao, H;Beck, BH;Mohammed, H;Arias, CR;Peatman, E;More than just antibodies: Protective mechanisms of a mucosal vaccine against fish pathogen Flavobacterium columnareFish Shellfish Immunol.28989091; 2000 instrument (Thermo Scientific, Wilmington, DE). RNA-seq library preparation and sequencing were carried out by the HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). Briefly, TruSeq RNA Sample PreparationFull Paper
2017Zhu, J;Fu, Q;Ao, Q;Tan, Y;Luo, Y;Jiang, H;Li, C;Gan, X;Transcriptomic profiling analysis of tilapia (Oreochromis niloticus) following Streptococcus agalactiae challengeFish Shellfish Immunol.28111359; For each timepoint, equal amount of RNA from individual fish were pooled together (15 fish) for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out in HudsonAlpha Genomic Services Lab (Huntsville, AL, USA)Full Paper
2017McCoy, CR;Jackson, NL;Day, J;Clinton, SM;Genetic predisposition to high anxiety- and depression-like behavior coincides with diminished DNA methylation in the adult rat amygdalaBehav. Brain Res.27965039; Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) for next-generation sequencing using NEBNext reagents (New England Biolabs, Ipswich, MA) according to manufacturer's recommendationsFull Paper
2017Stebbins, KJ;Broadhead, AR;Cabrera, G;Correa, LD;Messmer, D;Bundey, R;Baccei, C;Bravo, Y;Chen, A;Stock, NS;Prasit, P;Lorrain, DS;In vitro and in vivo pharmacology of NXT629, a novel and selective PPARα antagonistEur. J. Pharmacol.28483457expression of PPARα, normal healthy or melanoma skin biopsy samples (n=2 donors each) were obtained from ConversantBio (Hunstville, Alabama) Normal (n=5) and melanoma (n=4) human skin biopsy samples were obtained from Conversant Bio and expressionFull Paper
2017Pirie-Shepherd, SR;Painter, C;Whalen, P;Vizcarra, P;Roy, M;Qian, J;Franks, T;Coskran, T;Golas, J;Deng, S;Zhong, W;Tucker, E;Marrinucci, D;Gerber, HP;Powell, EL;Detecting expression of 5T4 in CTCs and tumor samples from NSCLC patientsPLoS ONE28727782... d from a variety of sources, including the Pfizer tumor bank and commercial vendors. All commercially sourced human biospecimens, including blood samples and matched tumor tissues, were obtained from Conversant Bio. Biospecimens distributed by CoFull Paper
2017Awasthi Mishra, N;Drögemüller, C;Jagannathan, V;Keller, I;Wüthrich, D;Bruggmann, R;Beck, J;Schütz, E;Brenig, B;Demmel, S;Moser, S;Signer-Hasler, H;Pieńkowska-Schelling, A;Schelling, C;Sande, M;Rongen, R;Rieder, S;Kelsh, RN;Mercader, N;Leeb, T;A structural variant in the 5'-flanking region of the TWIST2 gene affects melanocyte development in belted cattlePLoS ONE28658273Center Zurich, and the Genomic Services Lab at HudsonAlpha for performing experiments. Computationally intensive tasks were performed at the the Interfaculty Bioinformatics Unit of the University of Bern and the Vital-IT high-performance computing ceFull Paper
2017Carlson, DE;Hedin, M;Comparative transcriptomics of Entelegyne spiders (Araneae, Entelegynae), with emphasis on molecular evolution of orphan genesPLoS ONE28379977Next-generation sequencing technology is rapidly transforming the landscape of evolutionary biology, and has become a cost-effective and efficient means of collecting exome information for non-model organisms. Due to their taxonomic diversity, producFull Paper
2017Howard, LM;Hoek, KL;Goll, JB;Samir, P;Galassie, A;Allos, TM;Niu, X;Gordy, LE;Creech, CB;Prasad, N;Jensen, TL;Hill, H;Levy, SE;Joyce, S;Link, AJ;Edwards, KM;Cell-Based Systems Biology Analysis of Human AS03-Adjuvanted H5N1 Avian Influenza Vaccine Responses: A Phase I Randomized Controlled TrialPLoS ONE28099485Vanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, United States of America.++Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, NashvilFull Paper
2017O'Neil, BH;Wallmark, JM;Lorente, D;Elez, E;Raimbourg, J;Gomez-Roca, C;Ejadi, S;Piha-Paul, SA;Stein, MN;Abdul Razak, AR;Dotti, K;Santoro, A;Cohen, RB;Gould, M;Saraf, S;Stein, K;Han, SW;Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with advanced colorectal carcinomaPLoS ONE29284010mbedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek Molecular Laboratories, Goleta, CA, USA) [Full Paper
2017Danaee, H;Kalebic, T;Wyant, T;Fassan, M;Mescoli, C;Gao, F;Trepicchio, WL;Rugge, M;Consistent expression of guanylyl cyclase-C in primary and metastatic gastrointestinal cancersPLoS ONE29261789GCC IHC analysis and pathology assessment. GCC staining was performed on the Techmate Automated IHC Platform (BioTek Solutions/Ventana Medical Systems, Tucson, AZ, USA; QualTek Laboratories, Goleta, CA, USA) ;two independent pathologists. GCC IFull Paper
2017O'Neil, BH;Wallmark, JM;Lorente, D;Elez, E;Safety and antitumor activity of the anti–PD-1 antibody pembrolizumab in patients with advanced colorectal carcinomaPloS onearchived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory- developed prototype immunohistochemistry (IHC) assay (QualTek MolecuFull Paper
2017Eboigbodin, KE;Moilanen, K;Elf, S;Hoser, M;Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA®BMC Infect. Dis.28183291isothermal conditions. Methods. Clinical specimens and rapid processing. A total of 40 nasopharyngeal (NP) swab specimens were obtained from Discovery Life Sciences Biobank (Discovery Life Sciences, Inc., CA, USA). TheFull Paper
2017Liu, DT;Brewer, MS;Chen, S;Hong, W;Zhu, Y;Transcriptomic signatures for ovulation in vertebratesGen. Comp. Endocrinol.28111234PCR (qPCR) assay. 2.4. Library construction and Illumina sequencing. RNA-Seq library preparation and high-throughput NGS sequencing were carried out at HudsonAlpha Genomic Services Lab (Huntsville, AL). Qubit® 3.0Full Paper
2017Bhayana, S;Song, F;Jacob, J;Fadda, P;Denko, NC;Xu-Welliver, M;Chakravarti, A;Jacob, NK;Urinary miRNAs as Biomarkers for Noninvasive Evaluation of Radiation-Induced Renal Tubular InjuryRadiat. Res.28977780W81XWH-15-2-0054). We thank Dr. Jerry Nuovo of Phylogeny, Inc. (Powell, OH) for technical assistance. REFERENCES. 1. Hingorani S. Chronic kidney disease in long-term survivors of hematopoietic cell transplantation: epidemiology, pathogenesis, and treFull Paper
2017Kadić, E;Moniz, RJ;Huo, Y;Chi, A;Kariv, I;Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determinated by multiparametric single cell mass cytometry analysisBMC Immunol.28148223... ry detection antibodies Sample sourcing PBMCs were purchased from SeraCare Life Sciences Inc., (Milford, MA). Frozen dissociated tumor cells (DTC) and normal adjacent tissue (NAT) were purchased from ConversantBio (Huntsville, AL). The frozen samFull Paper
2017Parlato, M;Molenda, J;Murphy, WL;Specific recruitment of circulating angiogenic cells using biomaterials as filtersActa biomaterialia28373084CD133+ cells collected from mobilized peripheral blood were purchased from AllCells, LLC. These cells were collected by AllCells, LLC from one donor (26 year old, African American, SaveFull Paper
2017Block, TS;Murphy, TI;Munster, PN;Nguyen, DP;Lynch, FJ;Glucocorticoid receptor expression in 20 solid tumor types using immunohistochemistry assayCancer Manag Res28293120Tiffany I Murphy. 3 QualTek Molecular Laboratories, Newtown, PA. Find articles by Tiffany I Murphy. Pamela N Munster Dat P Nguyen. 1 Corcept Therapeutics, Inc, Menlo Park, CA. Find articles by Dat P Nguyen. Frank J Lynch. 3 QualTek Molecular LaboraFull Paper
2017Coletti, M;Hultquist, C;Kennedy, WG;Cervone, G;Validating Safecast data by comparisons to a U. S. Department of Energy Fukushima Prefecture aerial surveyJ Environ Radioact28167372Second, units are randomly selected, assembled, and undergo calibration tests at the Julich Research Centre in Germany, QualTek in the US, and the International Atomic Energy Agency (IAEA) testing laboratory in Seibersdorf, AustriaFull Paper
2017Thabet, AA;Maas, AE;Saber, SA;Tarrant, AM;Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposureMar Genomics28385518Total RNA was extracted from pools of C. limacina juveniles using the Aurum Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad). RNA purity and integrity were quantified using a Nanodrop ND-1000 spectrophotometer, and quality was assessed using a BioanaFull Paper
2017Matsushita, M;Ozawa, K;Suzuki, T;Nakamura, M;Nakano, N;Kanchi, S;Ichikawa, D;Matsuki, E;Sakurai, M;Karigane, D;Kasahara, H;Tsukamoto, N;Shimizu, T;Mori, T;Nakajima, H;Okamoto, S;Kawakami, Y;Hattori, Y;CXorf48 is a potential therapeutic target for achieving treatment-free remission in CML patientsBlood cancer journal28862699ty gradient centrifugation and cryopreserved in Cell Banker reagent (Juji-Field, Tokyo, Japan) in liquid nitrogen until further use. Bone marrow mononuclear cells (BMMNC) from HDs were purchased from AllCells, LLC. (Alameda, CA, USA). This study wasFull Paper
2017Simeonov, DR;Gowen, BG;Boontanrart, M;Roth, TL;Gagnon, JD;Mumbach, MR;Satpathy, AT;Lee, Y;Bray, NL;Chan, AY;Lituiev, DS;Nguyen, ML;Gate, RE;Subramaniam, M;Li, Z;Woo, JM;Mitros, T;Ray, GJ;Curie, GL;Naddaf, N;Chu, JS;Ma, H;Boyer, E;Van Gool, F;Huang, H;Liu, R;Tobin, VR;Schumann, K;Daly, MJ;Farh, KK;Ansel, KM;Ye, CJ;Greenleaf, WJ;Anderson, MS;Bluestone, JA;Chang, HY;Corn, JE;Marson, A;Discovery of stimulation-responsive immune enhancers with CRISPR activationNature28854172Normal donor human peripheral blood cells were obtained fresh from AllCells. CD4 + T cells were enriched from peripheral blood using the RosetteSep Human CD4 + T Cell SaveFull Paper
2017Kawamura, S;Onai, N;Miya, F;Sato, T;Tsunoda, T;Kurabayashi, K;Yotsumoto, S;Kuroda, S;Takenaka, K;Akashi, K;Ohteki, T;Identification of a Human Clonogenic Progenitor with Strict Monocyte Differentiation Potential: A Counterpart of Mouse cMoPsImmunity28514689Human UCB samples were provided by the Japanese Red Cross Kanto-Koshinetsu Cord Blood Bank, and human BM samples were purchased from AllCells. All experiments were SaveFull Paper
2017Peterson, VM;Zhang, KX;Kumar, N;Wong, J;Li, L;Wilson, DC;Moore, R;McClanahan, TK;Sadekova, S;Klappenbach, JA;Multiplexed quantification of proteins and transcripts in single cellsNature biotechnology28854175Cryopreserved PBMCs from a healthy donor (AllCells, PB003F, LotA5051) were thawed at 37 C for 3 min and resuspended in 10 ml of PBS with 1% Bovine Serum Albumin (BSA) ( SaveFull Paper
2017Zheng, C;Zheng, L;Yoo, JK;Guo, H;Zhang, Y;Guo, X;Kang, B;Hu, R;Huang, JY;Zhang, Q;Liu, Z;Dong, M;Hu, X;Ouyang, W;Peng, J;Zhang, Z;Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell SequencingCell28622514Anti-FOXP3 antibody (IHC) Abcam Cat#ab22510 CD3 Functional Grade Monoclonal Antibody eBioscience Cat#16-0037-85 CD28 Functional Grade Monoclonal Antibody eBioscience Cat#16-0289-85 Biological Samples Human PBMC AllCells https://www.allcells.comFull Paper
2017Sinha, G;The organoid architectScience (New York, N.Y.)28839056After almost a decade of tedious experiments, Clevers's postdoc Nick Barker struckgold in 2007: He discovered that cells carrying a receptor named LGR5 give rise to allcells in mouse SaveFull Paper
2017Stoeckius, M;Hafemeister, C;Stephenson, W;Houck-Loomis, B;Chattopadhyay, PK;Swerdlow, H;Satija, R;Smibert, P;Simultaneous epitope and transcriptome measurement in single cellsNature methods28759029ononuclear cells (CBMCs) were isolated from cord blood (New York Blood Center) as described25. Cells were kept on ice during and after isolation. Peripheral blood mononuclear cells were obtained from Allcells (USA). Comparing flow cytometry and CITEFull Paper
2017Seet, CS;He, C;Bethune, MT;Li, S;Chick, B;Gschweng, EH;Zhu, Y;Kim, K;Kohn, DB;Baltimore, D;Crooks, GM;Montel-Hagen, A;Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoidsNature methods28369043placental units from deliveries at UCLA. Bone marrow (BM) was obtained from healthy adult donors (ages 18-51) through discarded material from allogeneic BM donor harvests at UCLA or purchased from AllCells Inc. (Alameda, CA). G-CSF mobilized peripherFull Paper
2017Mumbach, MR;Satpathy, AT;Boyle, EA;Dai, C;Gowen, BG;Cho, SW;Nguyen, ML;Rubin, AJ;Granja, JM;Kazane, KR;Wei, Y;Nguyen, T;Greenside, PG;Corces, MR;Tycko, J;Simeonov, DR;Suliman, N;Li, R;Xu, J;Flynn, RA;Kundaje, A;Khavari, PA;Marson, A;Corn, JE;Quertermous, T;Greenleaf, WJ;Chang, HY;Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elementsNature genetics28945252Normal donor human peripheral blood cells were obtained fresh from AllCells. CD4 + T cells were enriched from peripheral blood using RosetteSep Human CD4 + T Cell Enrichment SaveFull Paper
2017Hao, H;Hu, S;Chen, H;Bu, D;Zhu, L;Xu, C;Chu, F;Huo, X;Tang, Y;Sun, X;Ding, BS;Liu, DP;Hu, S;Wang, M;Loss of Endothelial CXCR7 Impairs Vascular Homeostasis and Cardiac Remodeling After Myocardial Infarction: Implications for Cardiovascular Drug DiscoveryCirculation28154007Human umbilical vein endothelial cells were obtained from AllCells. The CXCL12 concentration was determined with a human CXCL12 ELISA kit (R&D Systems). Calcium release was SaveFull Paper
2017Gong, X;Litchfield, LM;Webster, Y;Chio, LC;Wong, SS;Stewart, TR;Dowless, M;Dempsey, J;Zeng, Y;Torres, R;Boehnke, K;Mur, C;Marugán, C;Baquero, C;Yu, C;Bray, SM;Wulur, IH;Bi, C;Chu, S;Qian, HR;Iversen, PW;Merzoug, FF;Ye, XS;Reinhard, C;De Dios, A;Du, J;Caldwell, CW;Lallena, MJ;Beckmann, RP;Buchanan, SG;Genomic Aberrations that Activate D-type Cyclins Are Associated with Enhanced Sensitivity to the CDK4 and CDK6 Inhibitor AbemaciclibCancer cell29232554All cell lines were obtained from commercial vendors and were cultured in conditions recommended by vendors (Table S8; AllCells: https://www.allcells.com/; ATCC: https://www.atcc.org/; CASTCC: http://www.cellbank.org.cn; Chempartner:Full Paper
2017McKeown, MR;Corces, MR;Eaton, ML;Fiore, C;Lee, E;Lopez, JT;Chen, MW;Smith, D;Chan, SM;Koenig, JL;Austgen, K;Guenther, MG;Orlando, DA;Lovén, J;Fritz, CC;Majeti, R;Superenhancer Analysis Defines Novel Epigenomic Subtypes of Non-APL AML, Including an RARα Dependency Targetable by SY-1425, a Potent and Selective RARα AgonistCancer discovery28729405Normal donor human blood cells were obtained fresh from AllCells (Alameda, CA) and FACS-enriched. Human AML samples were obtained from patients at the Stanford Medical Center with informed consent, according to Institutional Review Board (IRB)-approvFull Paper
2017Glass, JL;Hassane, D;Wouters, BJ;Kunimoto, H;Avellino, R;Garrett-Bakelman, FE;Guryanova, OA;Bowman, R;Redlich, S;Intlekofer, AM;Meydan, C;Qin, T;Fall, M;Alonso, A;Guzman, ML;Valk, PJM;Thompson, CB;Levine, R;Elemento, O;Delwel, R;Melnick, A;Figueroa, ME;Epigenetic Identity in AML Depends on Disruption of Nonpromoter Regulatory Elements and Is Affected by Antagonistic Effects of Mutations in Epigenetic ModifiersCancer discovery28408400based on sample availability. Twenty-two normal CD34+ bone marrow specimens were obtained from AllCells (Alameda, CA). In addition, 6 AML samples were obtained from the Memorial Sloan Kettering Hematologic Oncology Tissue Bank for colony forming assaFull Paper
2017Pandya, H;Shen, MJ;Ichikawa, DM;Sedlock, AB;Choi, Y;Johnson, KR;Kim, G;Brown, MA;Elkahloun, AG;Maric, D;Sweeney, CL;Gossa, S;Malech, HL;McGavern, DB;Park, JK;Differentiation of human and murine induced pluripotent stem cells to microglia-like cellsNature neuroscience28253233RNA from human dendritic and bone marrow macrophages (AllCells) was also used. Isolated total RNA was amplified with the WT Expression Kit (Ambion) and labeled with the SaveFull Paper
2017Rozo, C;Chinenov, Y;Maharaj, RK;Gupta, S;Leuenberger, L;Kirou, KA;Bykerk, VP;Goodman, SM;Salmon, JE;Pernis, AB;Targeting the RhoA-ROCK pathway to reverse T-cell dysfunction in SLEAnnals of the rheumatic diseases28283529In separate experiments, cryopreserved human cord blood CD4 + T lymphocytes from different donors were obtained from Allcells, LLC. CD4 + T-cell purity was >95%. Cells were SaveFull Paper
2017Miyawaki, K;Iwasaki, H;Jiromaru, T;Kusumoto, H;Yurino, A;Sugio, T;Uehara, Y;Odawara, J;Daitoku, S;Kunisaki, Y;Mori, Y;Arinobu, Y;Tsuzuki, H;Kikushige, Y;Iino, T;Kato, K;Takenaka, K;Miyamoto, T;Maeda, T;Akashi, K;Identification of unipotent megakaryocyte progenitors in human hematopoiesisBlood28336526Human adult bone marrow (BM) samples were obtained from healthy donors or purchased from AllCells. BM samples of adult hematological malignancies, diagnosed according to SaveFull Paper
2017Leong, SR;Sukumaran, S;Hristopoulos, M;Totpal, K;Stainton, S;Lu, E;Wong, A;Tam, L;Newman, R;Vuillemenot, BR;Ellerman, D;Gu, C;Mathieu, M;Dennis, MS;Nguyen, A;Zheng, B;Zhang, C;Lee, G;Chu, YW;Prell, RA;Lin, K;Laing, ST;Polson, AG;An anti-CD3/anti-CLL-1 bispecific antibody for the treatment of acute myeloid leukemiaBlood27908880(CD) CD8+ T cells were purified from peripheral blood of a healthy human donor (ALLCells, Alameda, CA) for donor 1 and a Genentech donor for donor 2 by Ficoll density gradient SaveFull Paper
2017Weiss, M;Genome Editing Strategies to Treat Beta-HemoglobinopathiesBloodFrozen CD34+ cells from mobilized peripheral blood of normal individuals were purchased from AllCells. Peripheral blood CD34+ cells were acquired from SCD patients undergoing therapeutic red cell exchange at the Cancer & Hematology Centers, Texas ChiFull Paper
2017Tisdale, J;Pierciey, F;Kamble, R;Kanter, J;Krishnamurti, L;Kwiatkowski, J;Thompson, A;Shestopalov, I;Bonner, M;Joseney-Antoine, M;Asmal, M;Walters, M;Successful Plerixafor-Mediated Mobilization, Apheresis, and Lentiviral Vector Transduction of Hematopoietic Stem Cells in Patients with Severe Sickle Cell DiseaseBloodBackground Patients with severe sickle cell disease (SCD) may benefit from β-globin gene transfer into autologous hematopoietic stem cells (HSC). Successful HBB gene transfer requires vector-mediated transduction of primitive HSCs. SteFull Paper
2017Fujii, S;Miura, Y;Fujishiro, A;Shindo, T;Shimazu, Y;Human Bone Marrow Mesenchymal Stromal/Stem Cell-Derived Extracellular Vesicles Ameliorate Graft-Versus-Host Disease-Related ManifestationsBloodHuman MSC were isolated from BM samples purchased from AllCells (Alameda, CA) based on our previously published method (Biochem Biophys Res Commun 2016;469:823). EV SaveFull Paper
2017Mathiyalagan, P;Liang, Y;Kim, D;Misener, S;Thorne, T;Kamide, CE;Klyachko, E;Losordo, DW;Hajjar, RJ;Sahoo, S;Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic HindlimbCirculation research28298297The cells were purified from mobilized peripheral blood mononuclear cells (AllCells LLC, Emeryville, CA) with an Isolex 300i device (Baxter Healthcare); cell purity (85% to 95%) was SaveFull Paper
2017Elagib, KE;Lu, CH;Mosoyan, G;Khalil, S;Zasadzińska, E;Foltz, DR;Balogh, P;Gru, AA;Fuchs, DA;Rimsza, LM;Verhoeyen, E;Sansó, M;Fisher, RP;Iancu-Rubin, C;Goldfarb, AN;Neonatal expression of RNA-binding protein IGF2BP3 regulates the human fetal-adult megakaryocyte transitionThe Journal of clinical investigation28481226The normal donor samples purchased from the Fred Hutchinson Cancer Research Center and AllCells were originally obtained with informed consent and IRB approval at both SaveFull Paper
2017Chung, SS;Eng, WS;Hu, W;Khalaj, M;Garrett-Bakelman, FE;Tavakkoli, M;Levine, RL;Carroll, M;Klimek, VM;Melnick, AM;Park, CY;CD99 is a therapeutic target on disease stem cells in myeloid malignanciesScience translational medicine28123069consent under IRB-approved protocols. Umbilical cord blood (CB) specimens were purchased from the New York Blood Center (New York, New York). Normal human BM specimens were purchased from AllCells Inc. (Emeryville, California)Full Paper
2017Zhang, T;Chi, S;Jiang, F;Zhao, Q;Xiao, B;A protein interaction mechanism for suppressing the mechanosensitive Piezo channelsNature communications29176668(MEM) containing 10% FBS, non-essential amino acids, 1 mM sodium pyruvate, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin. Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured using EGM-2 groFull Paper
2017Trabanelli, S;Chevalier, MF;Martinez-Usatorre, A;Gomez-Cadena, A;Salomé, B;Lecciso, M;Salvestrini, V;Verdeil, G;Racle, J;Papayannidis, C;Morita, H;Pizzitola, I;Grandclément, C;Bohner, P;Bruni, E;Girotra, M;Pallavi, R;Falvo, P;Leibundgut, EO;Baerlocher, GM;Carlo-Stella, C;Taurino, D;Santoro, A;Spinelli, O;Rambaldi, A;Giarin, E;Basso, G;Tresoldi, C;Ciceri, F;Gfeller, D;Akdis, CA;Mazzarella, L;Minucci, S;Pelicci, PG;Marcenaro, E;McKenzie, ANJ;Vanhecke, D;Coukos, G;Mavilio, D;Curti, A;Derré, L;Jandus, C;Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axisNature communications28928446Human CD34 + cells were purchased from either Lonza (CH) or AllCells (USA). NSG mice were purchased from The Jackson Laboratory (stock number: 005557) and bred, treated and SaveFull Paper
2017Zheng, GX;Terry, JM;Belgrader, P;Ryvkin, P;Bent, ZW;Wilson, R;Ziraldo, SB;Wheeler, TD;McDermott, GP;Zhu, J;Gregory, MT;Shuga, J;Montesclaros, L;Underwood, JG;Masquelier, DA;Nishimura, SY;Schnall-Levin, M;Wyatt, PW;Hindson, CM;Bharadwaj, R;Wong, A;Ness, KD;Beppu, LW;Deeg, HJ;McFarland, C;Loeb, KR;Valente, WJ;Ericson, NG;Stevens, EA;Radich, JP;Mikkelsen, TS;Hindson, BJ;Bielas, JH;Massively parallel digital transcriptional profiling of single cellsNature communications28091601s Jurkat (ATCC TIB-152), 293T (ATCC CRL-11268) and 3T3 (ATCC CRL-1658) cells were acquired from ATCC and cultured according to ATCC guidelines. Fresh PBMCs, frozen PBMCs and BMMCs were purchased from ALLCELLS. Frozen PBMCs from Donor A were made fromFull Paper
2017Nakata, R;Shimada, H;Fernandez, GE;Fanter, R;Fabbri, M;Malvar, J;Zimmermann, P;DeClerck, YA;Contribution of neuroblastoma-derived exosomes to the production of pro-tumorigenic signals by bone marrow mesenchymal stromal cellsJournal of extracellular vesicles28717423Human BM-derived MSCs were purchased from AllCells or isolated in our laboratory from the BM of patients with NB using a previously published protocol [10]. Cells were maintained SaveFull Paper
2017Burton, OT;Stranks, AJ;Tamayo, JM;Koleoglou, KJ;Schwartz, LB;Oettgen, HC;A humanized mouse model of anaphylactic peanut allergyThe Journal of allergy and clinical immunology27417025Cord blood-derived flow-sorted human CD34+ hematopoietic stem cells (HSCs) were purchased from AllCells (Alameda, Calif). Such cells are obtained under Institutional Review Board- or Human Subject Committee-approved protocols. Cells (5 × 104-105) werFull Paper
2017Bolisetty, M;Stitzel, M;Robson, P;CellView: Interactive exploration of high dimensional single cell RNA-seq databioRxivSingle cell RNA-seq - PBMCs were purchased from AllCells, thawed quickly at 37C and into DMEM supplemented with 10% FBS. Cells were quickly spun down at 400g, for 10min. SaveFull Paper
2017Li, S;Siriwon, N;Zhang, X;Yang, S;Jin, T;He, F;Kim, YJ;Mac, J;Lu, Z;Wang, S;Han, X;Wang, P;Enhanced Cancer Immunotherapy by Chimeric Antigen Receptor-Modified T Cells Engineered to Secrete Checkpoint InhibitorsClinical cancer research : an official journal of the American Association for Cancer Research28912137Chimeric antigen receptor (CAR) T-cell based therapy has shown promise as an immunotherapeutic modality for cancers. It has achieved successful responses in patients with certain SaveFull Paper
2017Dai, X;Yan, J;Fu, X;Pan, Q;Sun, D;Xu, Y;Wang, J;Nie, L;Tong, L;Shen, A;Zheng, M;Huang, M;Tan, M;Liu, H;Huang, X;Ding, J;Geng, M;Aspirin Inhibits Cancer Metastasis and Angiogenesis via Targeting HeparanaseClinical cancer research : an official journal of the American Association for Cancer Research28710312from the Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences); primary human umbilical vascular endothelial cells (HUVECs) were purchased from AllCells. CHO- SaveFull Paper
2017Das, DS;Das, A;Ray, A;Song, Y;Samur, MK;Munshi, NC;Chauhan, D;Anderson, KC;Blockade of Deubiquitylating Enzyme USP1 Inhibits DNA Repair and Triggers Apoptosis in Multiple Myeloma CellsClinical cancer research : an official journal of the American Association for Cancer Research28270494Purpose: The ubiquitin proteasome pathway is a validated therapeutic target in multiple myeloma. Deubiquitylating enzyme USP1 participates in DNA damage response and cellular SaveFull Paper
2017Liu, L;Lam, CK;Long, V;Widjaja, L;Yang, Y;Li, H;Jin, L;Burke, S;Gorlatov, S;Brown, J;Alderson, R;Lewis, MD;Nordstrom, JL;Koenig, S;Moore, PA;Johnson, S;Bonvini, E;MGD011, A CD19 x CD3 Dual-Affinity Retargeting Bi-specific Molecule Incorporating Extended Circulating Half-life for the Treatment of B-Cell MalignanciesClinical cancer research : an official journal of the American Association for Cancer Research27663593Cryopreserved purified primary CLL patient samples were from AllCells, LLC. Heparinized whole blood from cynomolgus monkeys was from Worldwide Primates, Inc. SaveFull Paper
2017DeRyckere, D;Lee-Sherick, AB;Huey, MG;Hill, AA;Tyner, JW;Jacobsen, KM;Page, LS;Kirkpatrick, GG;Eryildiz, F;Montgomery, SA;Zhang, W;Wang, X;Frye, SV;Earp, HS;Graham, DK;UNC2025, a MERTK Small-Molecule Inhibitor, Is Therapeutically Effective Alone and in Combination with Methotrexate in Leukemia ModelsClinical cancer research : an official journal of the American Association for Cancer Research27649555Deidentified cord blood and normal bone marrow samples were obtained commercially from Clinimmune Labs and ALLCELLS, respectively. SaveFull Paper
2017Stoeckius, M;Hafemeister, C;Stephenson, W;Houck-Loomis, B;Chattopadhyay, P;Swerdlow, H;Satija, R;Smibert, P;Large-scale simultaneous measurement of epitopes and transcriptomes in single cellsbioRxivCord blood mononuclear cells (CBMCs) were isolated from cord blood (New York Blood Center) as described30. Cells were kept on ice during and after isolation. Peripheral blood mononuclear cells were obtained from Allcells (USA)Full Paper
2017Cichocki, F;Valamehr, B;Bjordahl, R;Zhang, B;Rezner, B;Rogers, P;Gaidarova, S;Moreno, S;Tuininga, K;Dougherty, P;McCullar, V;Howard, P;Sarhan, D;Taras, E;Schlums, H;Abbot, S;Shoemaker, D;Bryceson, YT;Blazar, BR;Wolchko, S;Cooley, S;Miller, JS;GSK3 Inhibition Drives Maturation of NK Cells and Enhances Their Antitumor ActivityCancer research28790065Peripheral blood mononuclear cells (PBMCs) from healthy CMV seropositive donors were obtained from Memorial Blood Bank (Minneapolis, MN), AllCells Inc. (Alameda, CA) and Key Biologics (Memphis, TN). All samples obtained with written consent and wereFull Paper
2017Jiang, H;Rivera-Molina, Y;Gomez-Manzano, C;Clise-Dwyer, K;Bover, L;Vence, LM;Yuan, Y;Lang, FF;Toniatti, C;Hossain, MB;Fueyo, J;Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer VaccinationCancer research28566332Oncolytic viruses selectively lyse tumor cells, disrupt immunosuppression within the tumor, and reactivate antitumor immunity, but they have yet to live up to their therapeutic potential. SaveFull Paper
2017Chen, Y;Shan, Y;Lu, M;DeSouza, N;Guo, Z;Hoffman, R;Liang, A;Li, S;Alox5 Blockade Eradicates JAK2V617F-Induced Polycythemia Vera in MiceCancer research27784744GE Healthcare) and low-density mononuclear cells were separated after centrifugation. The CD34+ cell population was isolated using a human CD34+ cell selection kit (StemCell Technologies) according to the manufacturer's instructions. Normal human CD3Full Paper
2017Egidio, C;Gonzales, M;Brockman, J;Chen, S;Durruthy-Durruthy, R;Rogers, C;Ray, M;Abstract LB-078: Improved single-cell mRNA sequencing for transcriptome and paired-chain TCR analysis of primary human CD3+ T cellsCancer ResearchThe low RNA content of single human T cells has limited the success of single-cell transcriptome profiling, an approach that can generate data to reconstruct paired α,β T cell receptor (TCR) chains¹. Single-cell TCR sequencing is a powerful molecularFull Paper
2017Chen, Q;Lawrenz, A;Wang, A;Kelly, E;Harberts, E;Ernst, R;Tong, J;Abstract 4021: Donor-specific primary monocyte-derived dendritic cell maturation model for high-throughput screening of immunostimulatory adjuvantsCancer Research1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++2University of Maryland-Baltimore, Baltimore, MD.++2University of Maryland-Baltimore, Baltimore, MD.++1AllCells, Alameda, CAFull Paper
2017Han, X;Cinay, GE;Zhao, Y;Guo, Y;Zhang, X;Wang, P;Adnectin-Based Design of Chimeric Antigen Receptor for T Cell EngineeringMolecular therapy : the journal of the American Society of Gene Therapy28784559Human PBMCs from healthy donors were obtained from AllCells. PBMCs were cultured in T cell medium (TCM) consisting of X-Vivo 15 (Lonza) supplemented with 5% human AB serum SaveFull Paper
2017Tran, R;Myers, DR;Denning, G;Shields, JE;Lytle, AM;Alrowais, H;Qiu, Y;Sakurai, Y;Li, WC;Brand, O;Le Doux, JM;Spencer, HT;Doering, CB;Lam, WA;Microfluidic Transduction Harnesses Mass Transport Principles to Enhance Gene Transfer EfficiencyMolecular therapy : the journal of the American Society of Gene Therapy28780274vices and wells for at least 72 hr before assessing GFP expression of transduced cells with a BD C6 Accuri flow cytometer. Primary Human T Cell Culture Frozen human pan T cells were purchased from AllCells. Cells were thawed following the AllCells tFull Paper
2017Han, X;Bryson, PD;Zhao, Y;Cinay, GE;Li, S;Guo, Y;Siriwon, N;Wang, P;Masked Chimeric Antigen Receptor for Tumor-Specific ActivationMolecular therapy : the journal of the American Society of Gene Therapy28129121Frozen human peripheral blood mononuclear cells (PBMCs) were obtained from AllCells. PBMCs were thawed in TCM and rested overnight. Before retroviral transduction, PBMCs were SaveFull Paper
2017Liu, X;Rothe, K;Yen, R;Fruhstorfer, C;Maetzig, T;Chen, M;Forrest, DL;Humphries, RK;Jiang, X;A novel AHI-1-BCR-ABL-DNM2 complex regulates leukemic properties of primitive CML cells through enhanced cellular endocytosis and ROS-mediated autophagyLeukemia28366933PB or BM cells were obtained from newly diagnosed patients and healthy adult donors (ALLCELLS). Informed consent was obtained in accordance with the Declaration of Helsinki, and SaveFull Paper
2017Mosoyan, G;Kraus, T;Ye, F;Eng, K;Crispino, JD;Hoffman, R;Iancu-Rubin, C;Imetelstat, a telomerase inhibitor, differentially affects normal and malignant megakaryopoiesisLeukemia28270692Human MKs were generated using a two-step liquid culture system of either bone marrow (BM) or peripheral blood (PB) derived CD34+ cells (AllCells, LLC, Emeryville, CA, USA) as previously described.21,22 CFU-MK assays were performed by using the MegaCFull Paper
2017de Vasconcellos, JF;Tumburu, L;Byrnes, C;Lee, YT;Xu, PC;Li, M;Rabel, A;Clarke, BA;Guydosh, NR;Proia, RL;Miller, JL;IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblastsProceedings of the National Academy of Sciences of the United States of America28652347Digestive and Kidney Diseases Institutional Intramural Review Board. CD34(+) cells from adult human healthy volunteers and human cord blood CD34(+) cells obtained from AllCells were studied ex vivo using a serum-free 21-day culture system as previousFull Paper
2017Klinker, MW;Marklein, RA;Lo Surdo, JL;Wei, CH;Bauer, SR;Morphological features of IFN-γ-stimulated mesenchymal stromal cells predict overall immunosuppressive capacityProceedings of the National Academy of Sciences of the United States of America28283659PBMCs used in this study (AllCells; PB006F) were collected from healthy adult human donors. To activate T cells, 10 5 beads coated with anti-CD3 and anti-CD28 (Life Technologies; SaveFull Paper
2017Frescas, D;Roux, CM;Aygun-Sunar, S;Gleiberman, AS;Krasnov, P;Kurnasov, OV;Strom, E;Virtuoso, LP;Wrobel, M;Osterman, AL;Antoch, MP;Mett, V;Chernova, OB;Gudkov, AV;Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibodyProceedings of the National Academy of Sciences of the United States of America28193858Human NDFs were purchased from AllCells and maintained in DMEM supplemented with 10% (vol/vol) FBS serum, 100 units/mL of penicillin, 100 µg/mL of streptomycin, and 2 mM l- SaveFull Paper
2017Zhang, H;Gregorio, JD;Iwahori, T;Zhang, X;Choi, O;Tolentino, LL;Prestwood, T;Carmi, Y;Engleman, EG;A distinct subset of plasmacytoid dendritic cells induces activation and differentiation of B and T lymphocytesProceedings of the National Academy of Sciences of the United States of America28167780Stanford Blood Center. Human cord blood and bone marrow samples were purchased from AllCells. Human tonsil cells were obtained from the Stanford Tissue Bank. Human subjects protocols were approved by Stanford’s Institutional Review Board (Palo Alto,Full Paper
2017Nada, MH;Wang, H;Workalemahu, G;Tanaka, Y;Morita, CT;Enhancing adoptive cancer immunotherapy with Vγ2Vδ2 T cells through pulse zoledronate stimulationJournal for immunotherapy of cancer28239463random healthy adult donors were obtained by density centrifugation over Ficoll-Hypaque (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) of either random donor leukopaks (12 donors) (obtained from AllCells, LLC., Alameda, CA or the Blood Donor CentFull Paper
2017Kuramitsu, M;Sekizuka, T;Yamochi, T;Firouzi, S;Sato, T;Umeki, K;Sasaki, D;Hasegawa, H;Kubota, R;Sobata, R;Matsumoto, C;Kaneko, N;Momose, H;Araki, K;Saito, M;Nosaka, K;Utsunomiya, A;Koh, KR;Ogata, M;Uchimaru, K;Iwanaga, M;Sagara, Y;Yamano, Y;Okayama, A;Miura, K;Satake, M;Saito, S;Itabashi, K;Yamaguchi, K;Kuroda, M;Watanabe, T;Okuma, K;Hamaguchi, I;Proviral Features of Human T Cell Leukemia Virus Type 1 in Carriers with Indeterminate Western Blot Analysis ResultsJournal of clinical microbiology28701419AllCells Inc. (Alameda, CA, USA). Cryopreserved PBMCs were resuspended in RPMI 1640 supplemented with 10% FBS at 37C in accordance with the protocol provided by AllCells Inc. SaveFull Paper
2017Gonçalves Silva, I;Yasinska, IM;Sakhnevych, SS;Fiedler, W;Wellbrock, J;Bardelli, M;Varani, L;Hussain, R;Siligardi, G;Ceccone, G;Berger, SM;Ushkaryov, YA;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV;The Tim-3-galectin-9 Secretory Pathway is Involved in the Immune Escape of Human Acute Myeloid Leukemia CellsEBioMedicine28750861Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordance with the Save;ultured in Stem-Pro-34 serum-free media in the presence of 100 ng/ml SCF (KirshenbaFull Paper
2017Alvey, CM;Spinler, KR;Irianto, J;Pfeifer, CR;Hayes, B;Xia, Y;Cho, S;Dingal, PCPD;Hsu, J;Smith, L;Tewari, M;Discher, DE;SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid TumorsCurrent biology : CB28669759Subject 1: Female age 30, subject 2: Female age 31, subject 3: Male age 31, subject 4: Male age 22 with additional details provided by AllCells per (Figure 7A). Marrow was pooled and SaveFull Paper
2017Fujishiro, A;Miura, Y;Iwasa, M;Fujii, S;Sugino, N;Andoh, A;Hirai, H;Maekawa, T;Ichinohe, T;Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cellsInflammation and regeneration29259718e 5E10), and anti-CD105 (clone SN6) antibodies were from eBioscience (San Diego, CA). Isolation, expansion, and γ-irradiation of BM-MSCs BM samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA). BM-MSCs were isolated baFull Paper
2017Yang, C;Wang, X;Ma, B;Zhu, H;Huan, Z;Ma, N;Wu, C;Chang, J;3D-Printed Bioactive Ca3SiO5 Bone Cement Scaffolds with Nano Surface Structure for Bone RegenerationACS applied materials & interfaces28117976Rat bone-marrow stem cells (rBMSCs) (obtained from Allcells, Silicon Valley, CA) were seeded with an initial density of 1× 104 on each scaffold in 48 well tissue culture plates containing 500 μL of Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supFull Paper
2017Gautron, AS;Juillerat, A;Guyot, V;Filhol, JM;Dessez, E;Duclert, A;Duchateau, P;Poirot, L;Fine and Predictable Tuning of TALEN Gene Editing Targeting for Improved T Cell Adoptive ImmunotherapyMolecular therapy. Nucleic acids29246309eBioscience. All the cytometry analyses were performed on a FACSCanto II (BD Biosciences). Cell Culture Frozen peripheral blood mononuclear cells (PBMC) were obtained from healthy volunteer donors (Allcells). T lymphocytes were activated directly frFull Paper
2017Shim, H;Hypoxia-Inducible Factor-1Encyclopedia of CancerIn brief, CD34-depleted human cord blood mononuclear cells (#CB117; AllCells) were infected in vitro with the M81 strain of EBV (2,000 GRU) by incubation at 37C for 1.5 h after which SaveFull Paper
2017Zhang, W;Zhou, L;Shi, J;Deng, H;Fabrication of novel visible-light-driven AgI/g-C(3)N(4) composites with enhanced visible-light photocatalytic activity for diclofenac degradationJournal of colloid and interface science28222304Diclofenac sodium (DCF) was purchased from the Sigma Chemical Reagent Co., US Human umbilical vein endothelial cells (HUVECs) were obtained from Allcells, Shanghai, China. SaveFull Paper
2017Shi, J;Deng, H;Pan, H;Xu, Y;Zhang, M;Epigallocatechin-3-gallate attenuates microcystin-LR induced oxidative stress and inflammation in human umbilical vein endothelial cellsChemosphere27776235Primary HUVECs were obtained from Allcells (Shanghai, China) and cells in passages 3-5 were used in this study. Cells were grown in an RPMI 1640 medium containing 20% FBS ( SaveFull Paper
2017Ali, AJ;Abuelela, AF;Merzaban, JS;An Analysis of Trafficking Receptors Shows that CD44 and P-Selectin Glycoprotein Ligand-1 Collectively Control the Migration of Activated Human T-CellsFrontiers in immunology28515724Frozen normal peripheral blood mononuclear cells isolated from subjects with and without psoriasis were ordered from AllCells (CA, USA). Naive Pan T-cell Isolation Kit and Pan T-cell SaveFull Paper
2017Guo, H;Zhu, Q;Yu, X;Merugu, SB;Mangukiya, HB;Smith, N;Li, Z;Zhang, B;Negi, H;Rong, R;Cheng, K;Wu, Z;Li, D;Tumor-secreted anterior gradient-2 binds to VEGF and FGF2 and enhances their activities by promoting their homodimerizationOncogene28481872HUVECs were purchased from AllCells Company and maintained in HUVEC complete medium (AllCells, Shanghai, China). Cells were passaged for fewer than six passages. THP-1 SaveFull Paper
2017Winter, MB;La Greca, F;Arastu-Kapur, S;Caiazza, F;Cimermancic, P;Buchholz, TJ;Anderl, JL;Ravalin, M;Bohn, MF;Sali, A;O'Donoghue, AJ;Craik, CS;Immunoproteasome functions explained by divergence in cleavage specificity and regulationeLife29182146on the list of commonly misidentified cell lines from the International Cell Line Authentication Committee. Primary human peripheral blood mononuclear cells (PBMCs) (cat. PB005F) were purchased from AllCells (Alameda, CA). The SUP-B15 cell line was pFull Paper
2017Bak, RO;Dever, DP;Reinisch, A;Cruz Hernandez, D;Majeti, R;Porteus, MH;Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR/Cas9 and AAV6eLife28956530Frozen CD34+ HSPCs derived from mobilized peripheral blood or cord blood were purchased from AllCells (Alameda, CA, USA) and thawed according to manufacturer's instructions. SaveFull Paper
2017Kieffer, C;Ladinsky, MS;Ninh, A;Galimidi, RP;Bjorkman, PJ;Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomographyeLife28198699s used at a dilution of 1:500 and 10 nm gold-conjugated goat anti-rabbit IgG (Ted Pella) was used at a dilution of 1:20. Flow cytometry of primary and reconstituted human PBMCs Cultured human PBMCs (AllCells, inc.) were analyzed by flow cytometry (MFull Paper
2017Li, Y;Li, X;Zhao, R;Wang, C;Qiu, F;Sun, B;Ji, H;Qiu, J;Wang, C;Enhanced adhesion and proliferation of human umbilical vein endothelial cells on conductive PANI-PCL fiber scaffold by electrical stimulationMaterials science & engineering. C, Materials for biological applications28024565Human umbilical vein endothelial cells (HUVECs, AllCells Biological Technology Co., Ltd. Shanghai) were cultured in complete Medium. During the cell culture, the culture medium was refreshed every 2- 3 days. HUVECs at passage 2- 3 were used in this wFull Paper
2017Xu, JG;Zhu, SY;Heng, BC;Dissanayaka, WL;Zhang, CF;TGF-β1-induced differentiation of SHED into functional smooth muscle cellsStem cell research & therapy28114966f this study was to differentiate SHED into SMCs by TGF-β1 alone or in combination with BMP4, and to investigate the functionality of the derived SMCs. Methods Cell culture SHED were purchased from ALLCELLS (Alameda, CA, USA), and were evaluated forFull Paper
2017Schlatzer, D;Haqqani, AA;Li, X;Dobrowolski, C;Chance, MR;Tilton, JC;A Targeted Mass Spectrometry Assay for Detection of HIV Gag Protein Following Induction of Latent Viral ReservoirsAnalytical chemistry28467046Normal donor samples were obtained from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated using RosetteSep CD4+ T cell enrichment kit SaveFull Paper
2017Tan, S;Shan, Y;Wang, Y;Lin, Y;Liao, S;Deng, Z;Zhou, L;Cai, W;Zeng, Q;Zhang, L;Zhang, B;Men, X;Li, H;Hu, X;Wu, C;Peng, L;Lu, Z;Exacerbation of oxygen-glucose deprivation-induced blood-brain barrier disruption: potential pathogenic role of interleukin-9 in ischemic strokeClinical science (London, England : 1979)28550144The bEnd.3 cells were purchased from AllCells and grown to subconfluence before the exposure to oxygen-glucose deprivation (OGD: 94.95% N2, 0.05% O2, 5% CO2) or normoxic conditions (75% N2, 20% O2, 5% CO2) for 4 or 24 h. Cells were divided into the fFull Paper
2017Gregg, KA;Harberts, E;Gardner, FM;Pelletier, MR;Cayatte, C;Yu, L;McCarthy, MP;Marshall, JD;Ernst, RK;Rationally Designed TLR4 Ligands for Vaccine Adjuvant DiscoverymBio28487429The dendritic cell (DC) maturation experiments were contracted to and run by AllCells (Alameda, CA) using standard company protocols. Briefly, human PBMCs from 3 separate donors SaveFull Paper
2017Wang, K;Jiang, Z;Webster, KA;Chen, J;Hu, H;Zhou, Y;Zhao, J;Wang, L;Wang, Y;Zhong, Z;Ni, C;Li, Q;Xiang, C;Zhang, L;Wu, R;Zhu, W;Yu, H;Hu, X;Wang, J;Enhanced Cardioprotection by Human Endometrium Mesenchymal Stem Cells Driven by Exosomal MicroRNA-21Stem cells translational medicine28170197ngzhou, China, http://www.sebio.net.cn). Human AdMSCs were purchased from Cyagen Bioscience (Guangzhou, China, http://www.cyagen.com). Human umbilical vein endothelial cells (HUVECs) were provided by AllCells (Shanghai, China, http://www.allcells.comFull Paper
2017Sebastian, NT;Zaikos, TD;Terry, V;Taschuk, F;McNamara, LA;Onafuwa-Nuga, A;Yucha, R;Signer, RAJ;Riddell, J;Bixby, D;Markowitz, N;Morrison, SJ;Collins, KL;CD4 is expressed on a heterogeneous subset of hematopoietic progenitors, which persistently harbor CXCR4 and CCR5-tropic HIV proviral genomes in vivoPLoS pathogens28732051from each donor. All collected samples were coded. Whole umbilical cord blood (CB) from uninfected donors was obtained from the New York Blood Center and whole bone marrow was obtained commercially (AllCells Ltd.). All collected samples were anonymiFull Paper
2017Kraus, RJ;Yu, X;Cordes, BA;Sathiamoorthi, S;Iempridee, T;Nawandar, DM;Ma, S;Romero-Masters, JC;McChesney, KG;Lin, Z;Makielski, KR;Lee, DL;Lambert, PF;Johannsen, EC;Kenney, SC;Mertz, JE;Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoterPLoS pathogens28617871In brief, CD34-depleted human cord blood mononuclear cells (#CB117; AllCells) were infected in vitro with the M81 strain of EBV (2,000 GRU) by incubation at 37C for 1.5 h after which SaveFull Paper
2017Ganaie, SS;Zou, W;Xu, P;Deng, X;Kleiboeker, S;Qiu, J;Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complexPLoS pathogens28459842ti-viral prophylaxis of transplant recipients. Materials and methods Ethics statement We purchased CD34+ hematopoietic stem cells, which were isolated from bone marrow of a healthy human donor, from AllCells LLC (Alameda, CA) without any identificatFull Paper
2017Nie, Y;Ran, Y;Zhang, HY;Huang, ZF;Pan, ZY;Wang, SY;Wang, YY;GPATCH3 negatively regulates RLR-mediated innate antiviral responses by disrupting the assembly of VISA signalosomePLoS pathogens28414768University, Cambridge, MA). HEK293T (Transformed Human Embryonic Kidney 293 cell line, ATCC) cells, A549 (Human Lung Adenocarcinoma cell line, ATCC) cells, PBMCs (Peripheral Blood Mononuclear Cells, Allcells), Human recombinant TNFα (R&D Systems), poFull Paper
2017Xu, P;Zhou, Z;Xiong, M;Zou, W;Deng, X;Ganaie, SS;Kleiboeker, S;Peng, J;Liu, K;Wang, S;Ye, SQ;Qiu, J;Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathwayPLoS pathogens28264028We purchased the cells at AllCells LLC. (www.allcells.com, Alameda, CA), which were all anonymized, and, therefore, an IRB review was waived. SaveFull Paper
2017Morin, J;Dubey, N;Decroix, F;Luong-Van, E;Castro Neto, A;Rosa, V;Graphene transfer to 3-dimensional surfaces: a vacuum-assisted dry transfer method2D MaterialsDental pulp stem cells (DPSC) (AllCells, USA) of passage 4 were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen, USA), supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin/streptomycin (Invitrogen). Disinfection of the iFull Paper
2017Ma, SD;Tsai, MH;Romero-Masters, JC;Ranheim, EA;Huebner, SM;Bristol, JA;Delecluse, HJ;Kenney, SC;Latent Membrane Protein 1 (LMP1) and LMP2A Collaborate To Promote Epstein-Barr Virus-Induced B Cell Lymphomas in a Cord Blood-Humanized Mouse Model but Are Not EssentialJournal of virology28077657Commercially purchased CD34depleted human cord blood mononuclear cells (CB117; AllCells, LLC) were mock infected or infected with WT or mutant viruses in vitro for 1.5 h, and 12 SaveFull Paper
2017Adler, AS;Mizrahi, RA;Spindler, MJ;Adams, MS;Asensio, MA;Edgar, RC;Leong, J;Leong, R;Roalfe, L;White, R;Goldblatt, D;Johnson, DS;Rare, high-affinity anti-pathogen antibodies from human repertoires, discovered using microfluidics and molecular genomicsmAbs28846502For influenza A vaccinated libraries, we contracted AllCells to vaccinate three donors (ages For the pneumococcus vaccinated libraries, we contracted AllCells to vaccinate five donors ( SaveFull Paper
2017Urbinati, F;Wherley, J;Geiger, S;Fernandez, BC;Kaufman, ML;Cooper, A;Romero, Z;Marchioni, F;Reeves, L;Read, E;Nowicki, B;Grassman, E;Viswanathan, S;Wang, X;Hollis, RP;Kohn, DB;Preclinical studies for a phase 1 clinical trial of autologous hematopoietic stem cell gene therapy for sickle cell diseaseCytotherapy28733131Control healthy donor BM samples were purchased from commercial sources (AllCells or Hemacare) obtaining aspirates from volunteer donors. The ages of the five healthy donors SaveFull Paper
2017Pachler, K;Lener, T;Streif, D;Dunai, ZA;Desgeorges, A;Feichtner, M;Öller, M;Schallmoser, K;Rohde, E;Gimona, M;A Good Manufacturing Practice-grade standard protocol for exclusively human mesenchymal stromal cell-derived extracellular vesiclesCytotherapy28188071The second BM aspirate was purchased from AllCells. Both donors had signed an informed consent. BM-MSCs were isolated from BM as described [30] and tested negative for SaveFull Paper
2017Khairani, M;Nur, N;Basma, M;Widya, L;Solachuddin Jauhari, I;Deny, D;Azlina, A;Thirumulu, K;Effects of flaxseed extract on the viability of stem cells from human extracted deciduous teeth (SHED)Frontiers in Bioengineering and BiotechnologySHED (AllCells, USA) was previously cultured and maintained in alpha-MEM (GIBCO, USA) containing 10% FBS (GIBCO, USA) and 0.5% penicillin/streptomycin (GIBCO, USA) until SaveFull Paper
2017Lifshitz, V;Priceman, SJ;Li, W;Cherryholmes, G;Lee, H;Makovski-Silverstein, A;Borriello, L;DeClerck, YA;Yu, H;Sphingosine-1-Phosphate Receptor-1 Promotes Environment-Mediated and Acquired ChemoresistanceMolecular cancer therapeutics28716816Human BM-MSC were either purchased from ALLCells LLC or obtained from fresh ALLCells, which were maintained in basal medium with growth supplements obtained from ALLCells. SaveFull Paper
2017Zhao, H;Gulesserian, S;Ganesan, SK;Ou, J;Morrison, K;Zeng, Z;Robles, V;Snyder, J;Do, L;Aviña, H;Karki, S;Stover, DR;Doñate, F;Inhibition of Megakaryocyte Differentiation by Antibody-Drug Conjugates (ADCs) is Mediated by Macropinocytosis: Implications for ADC-induced ThrombocytopeniaMolecular cancer therapeutics28655784ABM026F) isolated from bone marrow were purchased from ALLCELLS. All HSC growth media (StemSpan SFEM II, catalog no. 09600), supplements for expansion (CC100, catalog no. SaveFull Paper
2017Ao, JY;Zhu, XD;Chai, ZT;Cai, H;Zhang, YY;Zhang, KZ;Kong, LQ;Zhang, N;Ye, BG;Ma, DN;Sun, HC;Colony-Stimulating Factor 1 Receptor Blockade Inhibits Tumor Growth by Altering the Polarization of Tumor-Associated Macrophages in Hepatocellular CarcinomaMolecular cancer therapeutics28572167A human umbilical vein endothelial cell (HUVEC) line was purchased from Allcells and was grown in Allcells completed medium supplemented with 10% FBS. All cell lines obtained SaveFull Paper
2017Zhao, H;Gulesserian, S;Malinao, MC;Ganesan, SK;Song, J;Chang, MS;Williams, MM;Zeng, Z;Mattie, M;Mendelsohn, BA;Stover, DR;Doñate, F;A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own DemiseMolecular cancer therapeutics28522588Human hematopoietic stem cells (CD133+, catalog no. ABM026F) isolated from bone marrow and human mature neutrophils (catalog no. PB016) were purchased from ALLCELLS. HSC growth media (StemSpan SFEM II, catalog no. 09600), supplements for expansion (CFull Paper
2017Tao, B;Wang, R;Sun, C;Zhu, Y;3-Mercaptopyruvate Sulfurtransferase, Not Cystathionine β-Synthase Nor Cystathionine γ-Lyase, Mediates Hypoxia-Induced Migration of Vascular Endothelial CellsFrontiers in pharmacology28979207et al., 2007). Materials and methods Cell culture Primary human umbilical vein endothelial cells (HUVECs) and culture medium (supplemented with or without 10% fetal bovine serum) were purchased from Allcells (Shanghai, China). Cells were cultured inFull Paper
2017Hall, BM;Balan, V;Gleiberman, AS;Strom, E;Krasnov, P;Virtuoso, LP;Rydkina, E;Vujcic, S;Balan, K;Gitlin, II;Leonova, KI;Consiglio, CR;Gollnick, SO;Chernova, OB;Gudkov, AV;p16(Ink4a) and senescence-associated β-galactosidase can be induced in macrophages as part of a reversible response to physiological stimuliAging28768895enmark) where live and dead counts of nucleated cells were quantitated on a NucleoCounter NC-200 (ChemoMetec) via staining with acridine orange and DAPI. Primary human neonatal dermal fibroblasts (AllCells, LLC; Alameda, CA), pooled equally from thFull Paper
2017Bam, RA;Hansen, D;Irrinki, A;Mulato, A;Jones, GS;Hesselgesser, J;Frey, CR;Cihlar, T;Yant, SR;TLR7 Agonist GS-9620 Is a Potent Inhibitor of Acute HIV-1 Infection in Human Peripheral Blood Mononuclear CellsAntimicrobial agents and chemotherapy27799218e anti-human CD8 antibodies, used for flow cytometry experiments, were purchased from BD Biosciences (San Jose, CA, USA) and stored at 4°C in the dark. Primary cells. Leucopacks were purchased from AllCells (Alameda, CA, USA) and were obtained fromFull Paper
2017Kota, DJ;Prabhakara, KS;Toledano-Furman, N;Bhattarai, D;Chen, Q;DiCarlo, B;Smith, P;Triolo, F;Wenzel, PL;Cox, CS;Olson, SD;Prostaglandin E2 Indicates Therapeutic Efficacy of Mesenchymal Stem Cells in Experimental Traumatic Brain InjuryStem cells (Dayton, Ohio)28233425Donors 3305, 3388 P0 MSC were isolated from commercially available fresh human bone marrow (hBM) aspirates (AllCells, Emeryville, CA, https://www.allcells.com/); MSC from donor TBM-1 were obtained from hBM aspirates under the approved IBC protocol #Full Paper
2017Diaz, MF;Vaidya, AB;Evans, SM;Lee, HJ;Aertker, BM;Alexander, AJ;Price, KM;Ozuna, JA;Liao, GP;Aroom, KR;Xue, H;Gu, L;Omichi, R;Bedi, S;Olson, SD;Cox, CS;Wenzel, PL;Biomechanical Forces Promote Immune Regulatory Function of Bone Marrow Mesenchymal Stromal CellsStem cells (Dayton, Ohio)28181347Bone marrow MSCs were derived from whole bone marrow from independent human donors (commercially available by AllCells) (Supporting Information Figure S1A). Briefly, mononuclear cells were enriched in the buffy layer of whole bone marrow by phase sepFull Paper
2017Garbuzova-Davis, S;Haller, E;Lin, R;Borlongan, CV;Intravenously Transplanted Human Bone Marrow Endothelial Progenitor Cells Engraft Within Brain Capillaries, Preserve Mitochondrial Morphology, and Display Pinocytotic Activity Toward Blood-Brain Barrier Repair in Ischemic Stroke RatsStem cells (Dayton, Ohio)28142208The isolation of human bone marrow endothelial progenitor cells (hBMEPCs) was performed accordingly to the protocol reported previously [52-54] with modifications. Briefly, for each independent experiment, human bone marrows (AllCells, Almeda, CA, htFull Paper
2017Zhang, M;Pan, H;Xu, Y;Wang, X;Qiu, Z;Jiang, L;Allicin Decreases Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Umbilical Vein Endothelial Cells through Suppression of Mitochondrial Dysfunction and Activation of Nrf2Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology28456799Commercial HUVECs were purchased from Allcells (Shanghai Biological Technology Co., Ltd., China). The cells were seeded in RPMI 1640 medium with 10 % fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 60?µg/mL endothelial cell growth supplemeFull Paper
2017Deng, H;Song, Z;Xu, H;Deng, X;Zhang, Q;Chen, H;Wang, Y;Qin, Y;Li, Y;MicroRNA-1185 Promotes Arterial Stiffness though Modulating VCAM-1 and E-Selectin ExpressionCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology28441665The pHUVECs were obtained from AllCells (Shanghai, China) and cultured in complete medium (AllCells, Shanghai, China). The HUVSMCs were purchased from ScienCell (Carlsbad, SaveFull Paper
2017Deng, H;Chu, X;Song, Z;Deng, X;Xu, H;Ye, Y;Li, S;Zhang, Q;Sun, C;Li, Y;MicroRNA-1185 Induces Endothelial Cell Apoptosis by Targeting UVRAG and KRIT1Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology28441650pHUVEC were purchased from Allcells (Shanghai, China) and maintained in complete medium (Allcells). The human endothelial cells line EA.hy926 cells (ATCC, Manassas, VA, USA) SaveFull Paper
2017Shay, AE;Diwakar, BT;Guan, BJ;Narayan, V;Urban, JF;Prabhu, KS;IL-4 up-regulates cyclooxygenase-1 expression in macrophagesThe Journal of biological chemistry28684424Frozen human PBMC were purchased from AllCells (Alameda, CA) from donors. Upon arrival, cells were thawed in a 37 °C water and enumerated with trypan blue staining using a hemocytometer to determine cell viability. Cells were transferred to a 50-ml tFull Paper
2017Li, X;Anderson, M;Collin, D;Muegge, I;Wan, J;Brennan, D;Kugler, S;Terenzio, D;Kennedy, C;Lin, S;Labadia, ME;Cook, B;Hughes, R;Farrow, NA;Structural studies unravel the active conformation of apo RORγt nuclear receptor and a common inverse agonism of two diverse classes of RORγt inhibitorsThe Journal of biological chemistry28546429Frozen CD4+ T cells (AllCells) were thawed and resuspended in X-VIVO media (Lonza) at a cell density of 1 × 106 cells/ml. Skewing cytokines were added to media at the final concentrations of 30 ng/ml IL-23, 10 ng/ml IL-1β, 10 ng/ml IL-6, 2 ng/ml IL-2Full Paper
2017Li, J;Bhattacharya, S;Zhou, J;Phadnis-Moghe, AS;Crawford, RB;Kaminski, NE;Aryl Hydrocarbon Receptor Activation Suppresses EBF1 and PAX5 and Impairs Human B LymphopoiesisJournal of immunology (Baltimore, Md. : 1950)28978690Fresh human CD34 + HSPCs isolated from cord blood from mixed donors were purchased from AllCells (Emeryville, CA). The in vitro feeder-free HSPC culture was modified based on a SaveFull Paper
2017Eskandarpour, M;Alexander, R;Adamson, P;Calder, VL;Pharmacological Inhibition of Bromodomain Proteins Suppresses Retinal Inflammatory Disease and Downregulates Retinal Th17 CellsJournal of immunology (Baltimore, Md. : 1950)28039300Enriched human PBMC-derived CD4+ T cells were obtained (AllCells; Caltag Medsystems, Buckingham, U.K.) and cultured in X-VIVO 20 and polarized to a Th17 phenotype by addition of recombinant human (rh)IL-6 (30 ng/ml), rhIL-23 (20 ng/ml), rhIL-1b (10 nFull Paper
2017Frescas, D;Hall, BM;Strom, E;Virtuoso, LP;Gupta, M;Gleiberman, AS;Rydkina, E;Balan, V;Vujcic, S;Chernova, OB;Gudkov, AV;Murine mesenchymal cells that express elevated levels of the CDK inhibitor p16(Ink4a) in vivo are not necessarily senescentCell cycle (Georgetown, Tex.)28650766Human NDFs were purchased from AllCells and maintained in DMEM supplemented with 10% FBS serum, 100 units/mL of penicillin, 100 µg/mL of streptomycin, and 2 mM L-Glutamine SaveFull Paper
2017Li, S;Ning, H;Ye, Y;Wei, W;Guo, R;Song, Q;Liu, L;Liu, Y;Na, L;Niu, Y;Chu, X;Feng, R;Moustaid-Moussa, N;Li, Y;Sun, C;Increasing extracellular Ca2+ sensitizes TNF-alpha-induced vascular cell adhesion molecule-1 (VCAM-1) via a TRPC1/ERK1/2/NFκB-dependent pathway in human vascular endothelial cellsBiochimica et biophysica acta. Molecular cell research28583863Primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Cells were used between passages 3 and 6. Human umbilical vein cell line (EA.hy926) and human leukemic monocyte lymphoma cell line (U937) were obtFull Paper
2017Wang, H;Gonzalez-Garcia, I;Traba, J;Jain, S;Conteh, S;Shin, DM;Qi, C;Gao, Y;Sun, J;Kang, S;Abbasi, S;Naghashfar, Z;Yoon, J;DuBois, W;Kovalchuk, AL;Sack, MN;Duffy, P;Morse, HC;ATP-degrading ENPP1 is required for survival (or persistence) of long-lived plasma cellsScientific reports29259245The efficiency of reconstitution was verified by FACS analyses of blood for frequencies of B and T cells shortly before mice were immunized 8 wks later. Human bone marrow cells were purchased from Allcells (Alameda, CA) and processed for antibody stFull Paper
2017Katano, I;Nishime, C;Ito, R;Kamisako, T;Mizusawa, T;Ka, Y;Ogura, T;Suemizu, H;Kawakami, Y;Ito, M;Takahashi, T;Long-term maintenance of peripheral blood derived human NK cells in a novel human IL-15- transgenic NOG mouseScientific reports29222435iminated twice using a MACS LD column (Miltenyi Biotec). The purity of the isolated human NK cells was usually greater than 95%. We also used human Caucasian PB-derived CD56+ NK cells, purchased from AllCells (Berkeley, CA, USA), in some experiments.Full Paper
2017Zhang, M;Qiu, L;Zhang, Y;Xu, D;Zheng, JC;Jiang, L;CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cellsScientific reports28811579Human umbilical cord vein endothelial cells (HUVECs) (AllCells, Shanghai, China) were cultured for up to 5-7 passages in complete endothelial cell growth medium (H004, AllCells, SaveFull Paper
2017Ma, R;Xie, R;Yu, C;Si, Y;Wu, X;Zhao, L;Yao, Z;Fang, S;Chen, H;Novakovic, V;Gao, C;Kou, J;Bi, Y;Thatte, HS;Yu, B;Yang, S;Zhou, J;Shi, J;Phosphatidylserine-mediated platelet clearance by endothelium decreases platelet aggregates and procoagulant activity in sepsisScientific reports28694452a50,51. Study was performed with the approval of the Ethics Committee of Harbin Medical University in accordance with the Declaration of Helsinki. Reagents Endothelial cell medium was purchased from AllCells (Emeryville, CA, USA). Bovine serum albumFull Paper
2017Garbuzova-Davis, S;Kurien, C;Thomson, A;Falco, D;Ahmad, S;Staffetti, J;Steiner, G;Abraham, S;James, G;Mahendrasah, A;Sanberg, PR;Borlongan, CV;Endothelial and Astrocytic Support by Human Bone Marrow Stem Cell Grafts into Symptomatic ALS Mice towards Blood-Spinal Cord Barrier RepairScientific reports28408761t 14 weeks of age and weekly thereafter, mice again underwent behavioral testing until 17 weeks of age. Cell preparation and transplant procedure Cryopreserved human bone marrow CD34+ cells (hBM34+, AllCells, Alameda, CA, USA) were thawed rapidly atFull Paper
2017Li, XH;Xue, WL;Wang, MJ;Zhou, Y;Zhang, CC;Sun, C;Zhu, L;Liang, K;Chen, Y;Tao, BB;Tan, B;Yu, B;Zhu, YC;H2S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expressionScientific reports28322298Primary human umbilical vein endothelial cells were purchased from Allcells (Shanghai, China) and cultured in endothelial complete medium (ECM; Allcells, China) as described SaveFull Paper
2017Fang, HY;Zeng, HW;Lin, LM;Chen, X;Shen, XN;Fu, P;Lv, C;Liu, Q;Liu, RH;Zhang, WD;Zhao, J;A network-based method for mechanistic investigation of Shexiang Baoxin Pill's treatment of cardiovascular diseasesScientific reports28272527HUVECs were seeded into 60 mm cell culture plates with complete endothelial growth medium (AllCells). After reaching 80% confluence, cells were exposed to SBP extract and SBPpc SaveFull Paper
2017Manser, M;Sater, MR;Schmid, CD;Noreen, F;Murbach, M;Kuster, N;Schuermann, D;Schär, P;ELF-MF exposure affects the robustness of epigenetic programming during granulopoiesisScientific reports28266526CD34-positive cells isolated from human cord blood of mixed donors were obtained from AllCells (Alabama, United States) and propagated in Stemline II medium expansion medium ( SaveFull Paper
2017Wei, G;Jie, Y;Haibo, L;Chaoneng, W;Dong, H;Jianbing, Z;Junjie, G;Leilei, M;Hongtao, S;Yunzeng, Z;Junbo, G;Dendritic cells derived exosomes migration to spleen and induction of inflammation are regulated by CCR7Scientific reports28223684ons (6 μm) were prepared using a Lab-Tek tissue processor (Leica). The samples were stained with CD11c/CD4 (Abcam) and DAPI and observed with confocal microscopy. CD4+ T cells were purchased from Allcells (Shanghai, China). To determine whether T ceFull Paper
2017Rodriguez, C;Kessler, F;Dubey, N;Rosa, V;Fechine, G;CVD graphene transfer procedure to the surface of stainless steel for stem cell proliferationSurface and Coatings TechnologyHuman dental pulp stem cells (DPSC) from a single donor (DPF003, Allcells, USA) were cultured in Dulbecco's modified Eagle's medium (Invitrogen, USA), supplemented with 10% SaveFull Paper
2017Lee, HJ;Diaz, MF;Ewere, A;Olson, SD;Cox, CS;Wenzel, PL;Focal adhesion kinase signaling regulates anti-inflammatory function of bone marrow mesenchymal stromal cells induced by biomechanical forceCellular signalling28647573Bone marrow MSCs were derived from whole bone marrow from independent human donor (AllCells). Cells were isolated and maintained as described previously [26]. Briefly, enriched SaveFull Paper
2017Killilea, DW;Rohner, F;Ghosh, S;Otoo, GE;Smith, L;Siekmann, JH;King, JC;Identification of a Hemolysis Threshold That Increases Plasma and Serum Zinc ConcentrationThe Journal of nutrition28490675Blood samples from 14 healthy participants (Supplemental Table 1) were purchased from a commercial laboratory (AllCells LLC). The laboratory prescreened donors for lack of SaveFull Paper
2017Bellayr, IH;Kumar, A;Puri, RK;MicroRNA expression in bone marrow-derived human multipotent Stromal cellsBMC genomics28800721A total of 8 human bone marrow-derived MSCs from 8 donors were purchased from ALLCELLS and Lonza at passage 1 (Additional file 1: Table S1). By flow cytometry, all MSCs SaveFull Paper
2017Huang, J;Khan, A;Au, BC;Barber, DL;López-Vásquez, L;Prokopishyn, NL;Boutin, M;Rothe, M;Rip, JW;Abaoui, M;Nagree, MS;Dworski, S;Schambach, A;Keating, A;West, ML;Klassen, J;Turner, PV;Sirrs, S;Rupar, CA;Auray-Blais, C;Foley, R;Medin, JA;Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34+ Cells for Correction of Fabry DiseaseMolecular therapy. Methods & clinical development28603745lutamine (PSQ) (2 mM glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin; Gibco by Life Technologies). Mobilized peripheral blood normal CD34+ cells from healthy donors were purchased from AllCells. Human primary CD34+ cells were cultured inFull Paper
2017Lawson, T;Kehoe, D;Schnitzler, A;Rapiejko, P;Der, K;Philbrick, K;Punreddy, S;Rigby, S;Smith, R;Feng, Q;Murrell, J;Rook, M;Process development for expansion of human mesenchymal stromal cells in a 50L single-use stirred tank bioreactorBiochemical Engineering JournalPBMCs (AllCells, Alameda, CA) that had been thawed and incubated overnight in 3 mL RPMI medium in ultra-low attachment 6-well plates (Corning) were stained with CFDA-SE (Thermo Fisher Scientific), according to the manufacturer’s recommendations. ForFull Paper
2017Heng, BC;Gong, T;Wang, S;Lim, LW;Wu, W;Zhang, C;Decellularized Matrix Derived from Neural Differentiation of Embryonic Stem Cells Enhances the Neurogenic Potential of Dental Follicle Stem CellsJournal of endodontics28231979The hESCs (WA01 line) were obtained from Wicell Institute (Madison, WI). Human DFSCs (catalog no. FT002 F, lot no. 020609) were purchased from AllCells LLC (Alameda, CA). Unless mentioned otherwise, all cell culture media and supplements were procureFull Paper
2017Elwood, F;Witter, DJ;Piesvaux, J;Kraybill, B;Bays, N;Alpert, C;Goldenblatt, P;Qu, Y;Ivanovska, I;Lee, HH;Chiu, CS;Tang, H;Scott, ME;Deshmukh, SV;Zielstorff, M;Byford, A;Chakravarthy, K;Dorosh, L;Rivkin, A;Klappenbach, J;Pan, BS;Kariv, I;Dinsmore, C;Slipetz, D;Dandliker, PJ;Evaluation of JAK3 Biology in Autoimmune Disease Using a Highly Selective, Irreversible JAK3 InhibitorThe Journal of pharmacology and experimental therapeutics28193636Frozen, purified human CD34+ from mobilized blood (AllCells) were seeded at 100,000 cell/ml in Stempro34 complete medium (Thermo Fisher) supplemented with GMCSF at 20 ng/ml (Thermo Fisher), human stem cell factor at 40 ng/ml (Cell Signaling), and IL-Full Paper
2017Erickson, RI;Schutt, LK;Tarrant, JM;McDowell, M;Liu, L;Johnson, AR;Lewin-Koh, SC;Hedehus, M;Ross, J;Carano, RA;Staflin, K;Zhong, F;Crawford, JJ;Zhong, S;Reif, K;Katewa, A;Wong, H;Young, WB;Dambach, DM;Misner, DL;Bruton's Tyrosine Kinase Small Molecule Inhibitors Induce a Distinct Pancreatic Toxicity in RatsThe Journal of pharmacology and experimental therapeutics27821712samples, sex unknown; Prodo Laboratories, Irvine, CA), universal RNA (rat/human; Clontech, Mountain View, CA), and total RNA from pooled isolated rat islet preparations (AllCells, SaveFull Paper
2017Ye, J;Si, J;Cui, Z;Wang, Q;Peng, K;Chen, W;Peng, X;Chen, S;Surface Modification of Electrospun TPU Nanofiber Scaffold with CNF Particles by Ultrasound-Assisted Technique for Tissue EngineeringMacromolecular Materials and EngineeringThe cell viability of the TPU and CNF-adsorbed TPU nanofiber was determined using a MTT assay (3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide). Human umbilical vein endothelial cells (HUVECs, AllCells Biological Technology Co., Ltd.,Full Paper
2017McBride, JD;Rodriguez-Menocal, L;Guzman, W;Candanedo, A;Garcia-Contreras, M;Badiavas, EV;Bone Marrow Mesenchymal Stem Cell-Derived CD63+ Exosomes Transport Wnt3a Exteriorly and Enhance Dermal Fibroblast Proliferation, Migration, and Angiogenesis In VitroStem cells and development28679315BM-MSC "line 2" and "line 3" were obtained from healthy male and female donors, respectively, according to quality control and manufacturing established by AllCells , and isolated SaveFull Paper
2017Setrerrahmane, S;Yu, J;Hao, J;Zheng, H;Xu, H;Novel production method of innovative antiangiogenic and antitumor small peptides in Escherichia coliDrug design, development and therapy29184391om GE Healthcare Life Sciences (Logan, UT, USA) and used for gene expression and protein purification, respectively. Human umbilical vein endothelial cells (HUVECs; primary cells) were purchased from AllCells (Shanghai, People’s Republic of China); aFull Paper
2017d Johnston, IC;Wagemaker, G;development of a highly efficient lentiviral gene transfer protocol for hematopoietic stem cellsBookBone marrow samples were purchased from AllCells (Alameda, USA). These studies were performed according to established ethical guidelines, and all blood donors gave informed SaveFull Paper
2017Ito, R;Nagai, D;Igo, N;Okuda, Y;Sekine, K;Ichimura, E;Katano, I;Mizushima, T;Goto, M;Ohnishi, Y;Ito, M;Okamoto, K;A novel in vivo model for predicting myelotoxicity of chemotherapeutic agents using IL-3/GM-CSF transgenic humanized miceToxicology letters28947270Commercially available human cord blood (CB)-derived CD34+ cells obtained from a single donor were purchased from Lonza (Basel, Switzerland) or Allcells (Alameda, CA, USA). The cells were prepared using a standard protocol for xenotransplantationFull Paper
2017FUKAHORI, H;Investigation Regarding the Properties of Protein Kinase Cθ Selective Inhibitor as a Novel T cell ImmunosuppressantThesisT cell proliferation assay Human peripheral T cells were purchased from AllCells LLC (Alameda, CA, USA). Cells (1 x 105) suspended in RPMI 1640 containing 10% fetal bovine serum SaveFull Paper
2017Jin, J;Wang, Y;Xu, Y;Zhou, X;Liu, Y;Li, X;Wang, J;MicroRNA-144 regulates cancer cell proliferation and cell-cycle transition in acute lymphoblastic leukemia through the interaction of FMN2The journal of gene medicine27556228ALL cell lines, Molt-3, Molt-4, Tall-104, Jurkat, CCRF-CEM, CEM/C1 and CEM/C2, were purchased from American Type Culture Collection (American Type Culture Collection, Manassas, VA, USA). A normal peripheral blood pan-T cell line (BPBPT) was purchasedFull Paper
2017Heck, BE;Park, JJ;Makani, V;Kim, EC;Kim, DH;PPAR-δ Agonist With Mesenchymal Stem Cells Induces Type II Collagen-Producing Chondrocytes in Human Arthritic Synovial FluidCell transplantation28901183To eliminate donor-to-donor variability, we used synovial fluid from 3 different OA patients and used BM-MSCs from 3 donors (AllCells) to measure the GAG content. The use of human SaveFull Paper
2017Bender, AT;Gardberg, A;Pereira, A;Johnson, T;Wu, Y;Grenningloh, R;Head, J;Morandi, F;Haselmayer, P;Liu-Bujalski, L;Ability of Bruton's Tyrosine Kinase Inhibitors to Sequester Y551 and Prevent Phosphorylation Determines Potency for Inhibition of Fc Receptor but not B-Cell Receptor SignalingMolecular pharmacology28062735E for 15 minutes and then measures CD63 upregulation by flow cytometry. Human cord blood isolated CD341 stem cells (AllCells, Alameda, CA) were differentiated to mast cells by culture for 8 weeks. Cells were maintained in StemPro-34 media (Gibco, WalFull Paper
2017Fujisawa, K;Takami, T;Fukui, Y;Quintanilha, LF;Matsumoto, T;Yamamoto, N;Sakaida, I;Evaluating effects of L-carnitine on human bone-marrow-derived mesenchymal stem cellsCell and tissue research28197778The studied BmMSCs were bone marrow cells purchased from Allcells and were continuously cultured and used at passage 4 and passage 16. Flow cytometry results confirmed that SaveFull Paper
2017Bai, L;Li, Q;Duo, X;Hao, X;Zhang, W;Shi, C;Guo, J;Ren, X;Feng, Y;Electrospun PCL-PIBMD/SF blend scaffolds with plasmid complexes for endothelial cell proliferationRSC Advances2.6.2. Cell culture.Human umbilical vein endothelial cells (HUVECs) were bought from Allcells Biomart (Shanghai, China) and cultured in Dulbecco's modified Eagle medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), 100 μgFull Paper
2017Dong, B;Zhang, X;Fan, Y;Cao, S;Zhang, X;GPR4 knockout improves renal ischemia-reperfusion injury and inhibits apoptosis via suppressing the expression of CHOPThe Biochemical journal29089376Cell culture and treatment Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCELLS (Emeryville, CA, U.S.A.). Cells were cultured in Dulbecco's modified Eagle medium (Gibco, U.S.A.) with 10% fetal bovine serum (Gibco, U.S.A.) at 3Full Paper
2017Li, S;Yuan, H;Pan, J;Fan, W;Zhu, L;Yan, Z;Guo, C;The treatment of femoral neck fracture using VEGF-loaded nanographene coated internal fixation screwsPloS one29117211The human umbilical vein endothelial cells (HUVECs) (Allcells, Alameda, CA, USA) and osteoblasts (Type culture collection of the Chinese Academy of Sciences, Shanghai, China) SaveFull Paper
2017Sewing, S;Roth, AB;Winter, M;Dieckmann, A;Bertinetti-Lapatki, C;Tessier, Y;McGinnis, C;Huber, S;Koller, E;Ploix, C;Reed, JC;Singer, T;Rothfuss, A;Assessing single-stranded oligonucleotide drug-induced effects in vitro reveals key risk factors for thrombocytopeniaPloS one291079690 sec after the end of the association phase in the binding sensorgram were evaluated to rank ONs. Human bone marrow toxicity assay Human mononucleated cells (hMNCs) were received cryopreserved from AllCells, LLC or STEMCELL Technologies Inc, and weFull Paper
2017Ross, SL;Sherman, M;McElroy, PL;Lofgren, JA;Moody, G;Baeuerle, PA;Coxon, A;Arvedson, T;Bispecific T cell engager (BiTE®) antibody constructs can mediate bystander tumor cell killingPloS one28837681Pan-T cells were purchased from AllCells. T cells that were not stimulated prior to use in assays are referred to as resting T cells. Activated T cells were generated by two methods: (1) SaveFull Paper
2017Liu, L;Koo, Y;Collins, B;Xu, Z;Sankar, J;Yun, Y;Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic systemPloS one28797069The product was purchased from Allcells with the concentration of 5 x 10 8 /ml. PRP was used immediately upon arrival. Micro-Renathane tubing (0.066-in. SaveFull Paper
2017Graci, JD;Michaels, D;Chen, G;Schiralli Lester, GM;Nodder, S;Weetall, M;Karp, GM;Gu, Z;Colacino, JM;Henderson, AJ;Identification of benzazole compounds that induce HIV-1 transcriptionPloS one28658263Brunswick, NJ (UMDNJ, now part of Rutgers University)[48]. ACH-2 and U1 cells were obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH. Cryopreserved PBMCs were purchased from AllCells, LLC (Alameda, CA). RPMI-1640, DMEM, hygrFull Paper
2017Salzberg, AC;Harris-Becker, A;Popova, EY;Keasey, N;Loughran, TP;Claxton, DF;Grigoryev, SA;Genome-wide mapping of histone H3K9me2 in acute myeloid leukemia reveals large chromosomal domains associated with massive gene silencing and sites of genome instabilityPloS one28301528Cryopreserved bone marrow CD34+ cells from normal donors were obtained from Allcells (Alameda, CA). Bone marrow CD34+ cells represent a combination of stem and progenitor SaveFull Paper
2017Li, Y;He, Q;Hu, X;Liu, Y;Cheng, X;Li, X;Deng, F;Improved performance of collagen scaffolds crosslinked by Traut's reagent and Sulfo-SMCCJournal of biomaterials science. Polymer edition28277011The HUVECs used in this study were purchased from Allcells (Shanghai). HUVECs were cultured with endothelial complete medium (Allcells, Shanghai) in an incubator with 5% CO 2 SaveFull Paper
2017Tharinger, H;Rebbapragada, I;Samuel, D;Novikov, N;Nguyen, MH;Jordan, R;Frey, CR;Pflanz, S;Antibody-dependent and antibody-independent uptake of HBsAg across human leucocyte subsets is similar between individuals with chronic hepatitis B virus infection and healthy donorsJournal of viral hepatitis28012213Peripheral blood from healthy donors (HD) was purchased from AllCells (Alameda, CA, USA). Informed written consent was obtained from all enrolled study subjects prior to blood draw. Additional details related to subject enrolment can be found in suppFull Paper
2017Bonar, MM;Tilton, JC;High sensitivity detection and sorting of infectious human immunodeficiency virus (HIV-1) particles by flow virometryVirology28235684This study was conducted according to the principles specified in the Declaration of Helsinki and under local ethical guidelines (Case Western Reserve University Institutional Review Board (IRB)). Normal donor samples were de-identified and obtainedFull Paper
2017Chen, S;Wu, X;Hu, J;Dai, G;Rong, A;Guo, G;PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cellsMolecular medicine reports28677750HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China). Cells were cultured in RPMI 1640 medium with 20% FBS, SaveFull Paper
2017DiCarlo, AL;Tamarat, R;Rios, CI;Benderitter, M;Czarniecki, CW;Allio, TC;Macchiarini, F;Maidment, BW;Jourdain, JR;Cellular Therapies for Treatment of Radiation Injury: Report from a NIH/NIAID and IRSN WorkshopRadiation research28605260having sources of ready-to-inject cells facilitates research. Although cells can be obtained from various commercial sources (e.g., Lonza, AllCells, Allosource), there is often variability in terms of how those cells grow in culture. There are governFull Paper
2017Liu, X;Jiang, Y;Wu, J;Zhang, W;Liang, Y;Jia, L;Yu, J;Jeong, LS;Li, L;NEDD8-activating enzyme inhibitor, MLN4924 (Pevonedistat) induces NOXA-dependent apoptosis through up-regulation of ATF-4Biochemical and biophysical research communications28450112HUVECs (Human umbilical vein endothelial cells) were obtained from Allcells Biotech Co. (Shanghai, China) and maintained in Dulbecco's Modified Eagle's Medium (GIBCO), containing 10% fetal bovine serum (FBS) and 1% Penicillin-Streptomycin solution, aFull Paper
2017Pittala, V;Vanella, L;Salerno, L;Di Giacomo, C;Acquaviva, R;Raffaele, M;Romeo, G;Modica, MN;Prezzavento, O;Sorrenti, V;Novel Caffeic Acid Phenethyl Ester (Cape) Analogues as Inducers of Heme Oxygenase-1Current pharmaceutical design28190391Frozen hMSCs, derived from bone marrow, were purchased from Allcells (Emeryville, CA). After thawing, cells were resuspended in α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 20% heat inactivated fetal bovine serum (FBFull Paper
2017Chan, RC;Kotner, JS;Chuang, CM;Gaur, A;Stabilization of pre-optimized multicolor antibody cocktails for flow cytometry applicationsCytometry. Part B, Clinical cytometry27001933RPMI 1640 medium (Hyclone), containing 10% fetal bovine serum (FBS), 2 mmol/L L-glutamine, and antibiotics. PBMCs used in long-term studies were stored in liquid nitrogen vapor. For some experiments, diseased and normal bone marrow samples were purchFull Paper
2017Dubey, N;Rajan, SS;Bello, YD;Min, KS;Rosa, V;Graphene Nanosheets to Improve Physico-Mechanical Properties of Bioactive Calcium Silicate CementsMaterials (Basel, Switzerland)28772959tion, and Bioactivity The use of human monoradicular premolars and dental pulp stem cells (DPSC) for research was approved by the Institutional Review Board/NUS (Approval Number: NUS 2094). The DPSC (Allcells, Alameda, CA, USA) between passage 3 andFull Paper
2017Seyfried, TF;Gruber, M;Pawlik, MT;Kasper, S;Mandle, RJ;Hansen, E;A new approach for fat removal in a discontinuous autotransfusion device-concept and evaluationVox sanguinis28960338Background Fat present during blood salvage in orthopaedic or cardiac surgery can pose a risk of fat embolism and should be eliminated before transfusion. Based on observations of SaveFull Paper
2017Fei, T;Shao, Y;Yan, Z;Zhu, L;Li, S;Pan, J;Guo, C;The effects of P-gp and CYP450 modulated by rifampicin on the steroid-induced osteonecrosis of the femoral headJournal of bone and mineral metabolism27848009MSCs (RBM-006, ALLCELLS) were added to 24-well plates and cultured for three passages. The MSCs were incubated with blank medium, medium supplemented with dexamethasone (10 μmol/l), and dexamethasone (10 μmol/l) plus rifampicin (5 μg/ml), each treatmFull Paper
2017Mao, M;Zheng, X;Jin, B;Zhang, F;Zhu, L;Cui, L;Effects of CD44 and E-cadherin overexpression on the proliferation, adhesion and invasion of ovarian cancer cellsExperimental and therapeutic medicine29285092CD44 is a prognostic indicator of shorter survival time in ovarian cancer. E‑cadherin fragmentation promotes the progression of ovarian cancer. However, the effects of CD44 and E‑ SaveFull Paper
2017Sutou, S;The 10th anniversary of the publication of genes and environment: memoir of establishing the Japanese environmental mutagen society and a proposal for a new collaborative study on mutagenic hormesisGenes and environment : the official journal of the Japanese Environmental Mutagen Society28265305Some chemicals probably are mutagenic or carcinogenic to virtually allcells of all organisms. Among these would be the unstable chemical species such as alkylating agents and other SaveFull Paper
2017He, L;Fan, F;Hou, X;Gao, C;Meng, L;Meng, S;Huang, S;Wu, H;Resveratrol suppresses pulmonary tumor metastasis by inhibiting platelet-mediated angiogenic responsesThe Journal of surgical research28629815Primary HUVECs were obtained from Allcells (Shanghai, China) and cultured in endothelial basal medium (Allcells) according to the manufacturer's protocol. SaveFull Paper
2017Iwasa, M;Miura, Y;Fujishiro, A;Fujii, S;Sugino, N;Yoshioka, S;Yokota, A;Hishita, T;Hirai, H;Andoh, A;Ichinohe, T;Maekawa, T;Bortezomib interferes with adhesion of B cell precursor acute lymphoblastic leukemia cells through SPARC up-regulation in human bone marrow mesenchymal stromal/stem cellsInternational journal of hematology28044259Human BM-MSCs were isolated from BM samples of healthy adults purchased from AllCells (Emeryville, CA, USA) based on our previously published method. A single-cell suspension SaveFull Paper
2017Suknuntha, K;Thita, T;Togarrati, PP;Ratanachamnong, P;Wongtrakoongate, P;Srihirun, S;Slukvin, I;Hongeng, S;Wnt signaling inhibitor FH535 selectively inhibits cell proliferation and potentiates imatinib-induced apoptosis in myeloid leukemia cell linesInternational journal of hematology27766528Mononuclear cells from CML patient in chronic phase were procured from AllCells (CA, USA). Histopaque-1077 (Sigma-Aldrich) density gradient centrifugation was performed on total SaveFull Paper
2017Hsieh, YT;Aggarwal, P;Cirelli, D;Gu, L;Surowy, T;Mozier, NM;Characterization of FcγRIIIA effector cells used in in vitro ADCC bioassay: Comparison of primary NK cells with engineered NK-92 and Jurkat T cellsJournal of immunological methods27939300HemaCare (HemaCare Corporation, Van Nuys, CA) and AllCells (AllCells, LLC, Alameda, CA), respectively. Engineered NK-92 (V-158) and NK-92 (F-158) effector cell lines were purchased under a license agreement from Conkwest. The parent cells were transfFull Paper
2017Trang, K;Raposo, RA;Lowe, MM;Krow-Lucal, ER;Yonemoto, W;Cabido, VD;SenGupta, D;McCune, JM;Relative mRNA Expression Levels of Restriction Factors and Antiviral Genes in Fetal and Adult Human Monocytes and Monocyte-Derived MacrophagesViral immunology28151065ABM samples were obtained from healthy donors (AllCells, LLC or Lonza, Inc.). Both adult and fetal mononuclear cells were isolated by density centrifugation of a Ficoll-Hypaque SaveFull Paper
2017Zhou, X;Liang, L;Zhao, Y;Zhang, H;Epigallocatechin-3-Gallate Ameliorates Angiotensin II-Induced Oxidative Stress and Apoptosis in Human Umbilical Vein Endothelial Cells through the Activation of Nrf2/Caspase-3 SignalingJournal of vascular research28942440HUVECs were obtained from Allcells Technology, China. The cells were seeded in RPMI 1640 medium with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 60 μg/mL of endothelial cell growth supplement (BD, San Diego, CA, USA) in an incubator atFull Paper
2017Goto, K;Goto, M;Ando-Imaoka, M;Kai, K;Mori, K;Evaluation of drug-induced hematotoxicity using novel in vitro monkey CFU-GM and BFU-E colony assaysThe Journal of toxicological sciences28717098Commercial leukopaks generally contain 80-200 mL of processed material with approximately 7x109peripheral blood mononuclear cells (PBMC), (more information available on: www.allcells.com/products/whole-tissue/leuko-pak). The Table I shows the percentFull Paper
2017Gao, X;Yourick, JJ;Sprando, RL;Comparative transcriptomic analysis of endothelial progenitor cells derived from umbilical cord blood and adult peripheral blood: Implications for the generation of induced pluripotent stem cellsStem cell research29156376Frozen human MNCs from umbilical CB and adult PB of healthy individuals were obtained from AllCells (Alameda, CA). The cells were thawed, washed, and resuspended in EGM-2 SaveFull Paper
2017Enezei, H;Ahmad, A;Khamis, M;Suzuki, J;Sugita, Y;Maeda, H;Alshehadat, S;Razak, N;Abbas, S;Qabbani, A;Alam, M;Enhanced Osteogenic and Angiogenic-Related Gene Expression of Human Dental Stem Cells on Biphasic Calcium Phosphate Scaffold Treated with Vascular Endothelial Growth Factor: Part IJournal of Hard Tissue BiologyCell culture: Commercially obtained DSCs from (AllCells, LLC Company, Emeryville, CA, USA) were cultured in the Dulbecco's Modifi ed Eagle Medium (DMEM) high glucose, (4.5g/l), Fetal bovine serum (FBS), Dulbecco's PBS (without Ca+2 and Mg+2), PenicilFull Paper
2017Jasińska-Stroschein, M;Orszulak-Michalak, D;Novel strategies for treatment of pulmonary arterial hypertensionPostepy higieny i medycyny doswiadczalnej (Online)287919522.3.2 EARLY OUTGROWTH EPC ISOLATION Human early EPCs were generated from leukapheresis samples obtained from Leuko-Paks purchased from AllCells, inc (Almeda, California, USA) or obtained from healthy donors in accordance with the Ottawa Hospital ReseFull Paper
2017Cheng, Z;Yi, Y;Xie, S;Yu, H;Peng, H;Zhang, G;The effect of the JAK2 inhibitor TG101209 against T cell acute lymphoblastic leukemia (T-ALL) is mediated by inhibition of JAK-STAT signaling and activation of the crosstalk between apoptosis and autophagy signalingOncotarget29290986(A) HSD2, peer and bone marrow primary T-ALLcells were treated with increasing concentrations of TG101209 for 48 h, which was followed by analysis of apoptosis by staining with PI SaveFull Paper
2017Chen, C;Li, H;Jiang, J;Zhang, Q;Yan, F;Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD ratsOncotarget29069818t have structural similarities to the human body to obtain more precise results. MATERIALS AND METHODS Cultivation of BMMSCs Bone marrow mesenchymal stem cell (BMMSCs) of SD rats were purchased from AllCells, LLC (Alameda, CA, USA), isolated and purFull Paper
2017Brin, E;Wu, K;Lu, HT;He, Y;Dai, Z;He, W;PEGylated arginine deiminase can modulate tumor immune microenvironment by affecting immune checkpoint expression, decreasing regulatory T cell accumulation and inducing tumor T cell infiltrationOncotarget28938609richia coli. The recombinant protein is PEGylated with 20,000 MW PEG [40-42]. C397A substitution was used to generate the inactive ADI-PEG 20 mutant. Frozen healthy donor PBMCs were purchased from AllCells. Cancer cell lines AGS, A2780, A375, HCT116Full Paper
2017Horn, LA;Ciavattone, NG;Atkinson, R;Woldergerima, N;Wolf, J;Clements, VK;Sinha, P;Poudel, M;Ostrand-Rosenberg, S;CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1+ tumor cells, and extends the survival of tumor-bearing humanized miceOncotarget28938530PBMC used for NKT purification were obtained from AllCells, LLC (Alameda, CA) and were handled according to the manufacturer's direction. Fresh samples of blood from SCLC and SaveFull Paper
2017Bilger, A;Plowshay, J;Ma, S;Nawandar, D;Barlow, EA;Romero-Masters, JC;Bristol, JA;Li, Z;Tsai, MH;Delecluse, HJ;Kenney, SC;Leflunomide/teriflunomide inhibit Epstein-Barr virus (EBV)- induced lymphoproliferative disease and lytic viral replicationOncotarget28574826id cell line (LCL) derived from the peripheral blood leukocytes of anonymous donors, obtained from the American Red Cross [55]. M81-Luc LCLs were obtained by infecting CD34-depleted human cord blood (AllCells) with M81-Luc virus, described below, andFull Paper
2017Estupina, P;Fontayne, A;Barret, JM;Kersual, N;Dubreuil, O;Le Blay, M;Pichard, A;Jarlier, M;Pugnière, M;Chauvin, M;Chardès, T;Pouget, JP;Deshayes, E;Rossignol, A;Abache, T;de Romeuf, C;Terrier, A;Verhaeghe, L;Gaucher, C;Prost, JF;Pèlegrin, A;Navarro-Teulon, I;The anti-tumor efficacy of 3C23K, a glyco-engineered humanized anti-MISRII antibody, in an ovarian cancer model is mainly mediated by engagement of immune effector cellsOncotarget28427157Ficoll procedure. Human and cynomolgus monkey PBMC were frozen in FBS with 10% DMSO and stored in liquid nitrogen until use. Murine PBMC isolated from C57BL/6 mice were directly purchased frozen from AllCells (Alameda, CA, USA). 51Cr-labeled COV434-MFull Paper
2017Diaz, MF;Evans, SM;Olson, SD;Cox, CS;Wenzel, PL;A Co-culture Assay to Determine Efficacy of TNF-α Suppression by Biomechanically Induced Human Bone Marrow Mesenchymal Stem CellsBio-protocol28932763Human bone marrow (BM) MSC (Whole Bone Marrow aspirates) (AllCells, catalog number: ABM001-0) MSCs were isolated from whole bone marrow using a Ficoll gradient followed by plastic adherence and then cultured in MSC media (see Recipes)Full Paper
2017Noorani, TY;Luddin, N;Rahman, IA;Masudi, SM;In Vitro Cytotoxicity Evaluation of Novel Nano-Hydroxyapatite-Silica Incorporated Glass Ionomer CementJournal of clinical and diagnostic research : JCDR28571275Mesenchymal stem cell (MSC) Basal Medium and supplements were purchased from ALLCELLS (USA). Tryp LE express was purchased from Invitrogen (California, USA). For MTT test, SaveFull Paper
2017Wang, J;Zhang, Y;Zhu, Q;Liu, Y;Cheng, H;Zhang, Y;Li, T;Data on the radioprotective effect of emodin in vivo and vitro via inhibition of apoptosis and modulation of p53Data in brief28275662aterials and methods 2.1 Experimental design and materials Male C57BL/6 mice were purchased from SIPPR-BK Experiment Animal Co. (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) from ALLCells, LLC (Emeryville, CA, USA). Emodin (puritFull Paper
2017Ahmed, H;Luddin, N;Kannan, T;Mokhtar, K;Ahmad, A;Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cellsEuropean Journal of General Dentistrymolars (adult) and characterized was purchased from AllCells (MD, USA) and was used in (AllCells), with 50 ml of human mesenchymal stem cell stimulatory supplements (AllCells). SaveFull Paper
2017张文博, ;张贤, ;Influence of M2 macrophage supernatant combined with Eucommia flavonoids on the biological behavior of osteoblasts under hypoxic conditionsChinese Journal of Tissue Engineering Research: 文 快速阅读: 文题释义: M2 型巨噬细胞: 即替代活化巨噬细胞, 由白细胞介 4, 白细胞介 13 诱导, 可使甘露糖受体及转谷氨酰胺酶2 表达上调. M2 型巨噬细胞及其分泌 子可以促进组织 Save;METHODS:AfterMC3T3E1cellswereresuscitated,andM2macrophageswereisolated,inducedandcultured,allcells weredividedintocontrol,M2macrophage, SaveFull Paper
2017Richardson, C;Mechanistic and Therapeutic Studies Related to Anemia of Chronic Disease and InflammationUniversity of Virginiafollows: IFNγ 1500U/ml, TNFα 100ng/ml, IL-1β 100ng/ml, IL-6 100ng/ml IL-10 100ng/ml, IL-15 100ng/ml, and LPS 50ug/ml (PeproTech). Purified human CD36+ cells (AllCells LLC, Emeryville, CA) underwent expansion as previously described [73] for 48 hoursFull Paper
2017Alvey, C;Engineered Marrow Macrophages For Cancer Therapy: Engorgement, Accumulation, Differentiation, And Acquired ImmunityThesisHuman Specimens: Fresh human bone marrow was purchased from AllCells (Cat#: ABM001-1) in 3 mL quantities from healthy donors. AllCells received IRB approval from Alpha (Protocol #7000-SOP-046) which includes donor consent. In total, there were 4 diffFull Paper
2017Walters, MC;Hongeng, S;Kwiatkowski, JL;Locatelli, F;Results from the Hgb-207 (Northstar-2) Trial: A Phase 3 Study to Evaluate Safety and Efficacy of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia (TDT) in Patients with Non-β0/β0 GenotypesBloodoad : Download full-size image [https://ars.els-cdn.com/content/image/1-s2.0-S000649711981042X-gr1.jpg] DISCLOSURES WALTERS: _bluebird bio:_ Research Funding; _Sangamo Therapeutics:_ Consultancy; _AllCells, Inc:_ Other: Medical Director; _ViaCordFull Paper
2017Talano, JA;Moore, TB;Keever-Taylor, CA;Shenoy, S;Promising Results at 1 Year Follow-up Following Familial Haploidentical (FHI) T-Cell Depleted (TCD) with CD34 Enrichment and T-Cell (CD3) Addback Allogeneic Stem Cell Transplantation in Patients with High-Risk Sickle Cell Disease (SCD) (IND 14359)Bloodls-cdn.com/content/image/1-s2.0-S0006497119851182-gr1_lrg.jpg] * Download : Download full-size image [https://ars.els-cdn.com/content/image/1-s2.0-S0006497119851182-gr1.jpg] DISCLOSURES WALTERS: _AllCells, Inc:_ Other: Medical Director; _bluebirdFull Paper
2017Acharya, S;Defining Immune Cell Profiles and their Role in Predicting Therapeutic Response in Multiple MyelomaThesisDana-Farber Cancer Institute. These samples were collected after informed consent in accordance with the Declaration of Helsinki and approved by the institutional review board of the Dana-Farber Cancer Institute. Healthy donor samples were obtained fFull Paper
2017Accomando, WP;Wiencke, JK;Houseman, EA;HousemanEugenePHHSQuantitativeReconstructionLeukocyte. pdfGenome Biologyblood, that were utilized in this work. Blood was obtained through an institutional review board-approved donor program at AllCells, LLC (Alameda, CA, USA) and research was carried out in accordance with the Helsinki declaration. Samples were de-idenFull Paper
2017Zhang, Y;Farhangfar, F;Giblin, L;Wolf, D;Vitelli, F;Assessment of Differentiation States of Hematopoietic Stem Cells Following in Vitro Culture Using Side and Forward Scatter of Flow CytometryWorld Journal of Research and ReviewG-CSF mobilized peripheral HSC (AllCells, LLC. Emeryville, CA) were cultured with CellGro®Stem Cell Expansion Media (CellGenix, Inc., Catalog number: 2001) containing 10ng/ml TPO (Peprotech, Inc. Rocky Hill, NJ. Catalog number: 300-18), 50ng/ml SCFFull Paper
2017Gregg, KA;Novel Lipid A Structures for Adjuvant Discovery and DevelopmentThesis100 IU/mL penicillin, 100 µg/mL streptomycin, 1 mM sodium pyruvate, and 200 mM l-glutamine. THP-1 (ATCC, Manassas, VA), primary mouse splenocytes, and primary human PBMCs (AllCells, LLC, Alameda, CA) were cultured in RPMI 1640Full Paper
2017Edun, FA;Endothelial cell response to bioactive peptides and stiffened extracellular matrices: an investigation into atherogenic pathwaysThesisexample, samples delivered from AllCells (California, U.S.A.) had markedly different culture adherence and proliferation characteristics (cell doubling time) than HUVECs obtained from ThermoFisher, prompting our repeated purchase of only batch numberFull Paper
2017Bonar, MM;Rapid Enumeration, Sorting and Maturation Analysis of Single Viral Particles in HIV-1 Swarms by High-resolution Flow VirometryThesisidentified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated by adding additional autologous red blood cells to leukapheresis samples and RosetteSep CD4+ T cell enrichment kFull Paper
2017Basiorka, AA;Changing the pathobiological paradigm in myelodysplastic syndromes: The NLRP3 inflammasome drives the MDS phenotypeThesisNormal, heparinized BM aspirates were purchased from Lonza Walkersville or AllCells, LLC. Normal and MDS BM mononuclear cells (BMMNC) were isolated from heparinized BM aspirates using Ficoll-Hypaque Plus gradient centrifugation (GE Healthcare). RecomFull Paper
2017Bennett, LF;Analysis of Stress Erythropoiesis during Inflammation: Stimulation of Toll-Like Receptors Induces Erythrophagocytosis and Activates Stress ErythropoiesisThesisAfter 24 hours, cells were washed and replated with fresh SEEM. Human bone marrow mononuclear cells were purchased from ReachBio (0300-200) and cultured in SEEM containing human insulin, human holo-transferrin, and human cytokines. Human MNCs cultureFull Paper
2017Newkirk, SJ;Lee, S;Grandi, FC;Gaysinskaya, V;Rosser, JM;Vanden Berg, N;Hogarth, CA;Marchetto, MCN;Muotri, AR;Griswold, MD;Ye, P;Bortvin, A;Gage, FH;Boeke, JD;An, W;Intact piRNA pathway prevents L1 mobilization in male meiosisProceedings of the National Academy of Sciences of the United States of America28630288wrote the paper. Reviewers: P.D., Tulane Cancer Center; and P.J.W., University of Pennsylvania. 1Present address: Cancer Biology Program, Stanford University, Stanford, CA 94305. 2Present address: ReachBio LLC, Seattle, WA 98107. 3Present addressFull Paper
2017Zhen, A;Peterson, CW;Carrillo, MA;Reddy, SS;Youn, CS;Lam, BB;Chang, NY;Martin, HA;Rick, JW;Kim, J;Neel, NC;Rezek, VK;Kamata, M;Chen, ISY;Zack, JA;Kiem, HP;Kitchen, SG;Long-term persistence and function of hematopoietic stem cell-derived chimeric antigen receptor T cells in a nonhuman primate model of HIV/AIDSPLoS pathogens29284044Following conditioning, the HSPC product was infused back into the autologous animal. A small aliquot of the infused cell product was plated in Colony Gel Medium (Reach Bio, Seattle, WA) and analyzed as previously described[14,39]. Individual colonieFull Paper
2017Liu, FD;Pishesha, N;Poon, Z;Kaushik, T;Van Vliet, KJ;Material Viscoelastic Properties Modulate the Mesenchymal Stem Cell Secretome for Applications in Hematopoietic RecoveryACS biomaterials science & engineering33445371Human bone marrow-derived MSCs (MSCs) were purchased from multiple commercial sources (Lonza, RoosterBio, and ReachBio) at what was stated by the vendors to be a low SaveFull Paper
2017Hinerfeld, D;Fuhrman, K;Demirkan, G;DosSantos, G;Geiss, G;A simple and integrated workflow for deep proteomic and transcriptomic analysis of sorted cell populationsThe Journal of Immunology1Nanostring Technologies++1Nanostring Technologies++1Nanostring Technologies++2Reachbio Research Labs++1Nanostring TechnologiesFull Paper
2017Min, C;Moore, N;Shearstone, JR;Quayle, SN;Huang, P;van Duzer, JH;Jarpe, MB;Jones, SS;Yang, M;Selective Inhibitors of Histone Deacetylases 1 and 2 Synergize with Azacitidine in Acute Myeloid LeukemiaPloS one28060870Specifically, compounds were dissolved in DMSO and added to tubes of methylcellulose-based medium (Reachbio, catalog 1103) containing cytokine formulations to support colony Save;row samples from AML patients were treated with ACY-957 alone, azacitFull Paper
2017Kudrimoti, M;Curtis, A;Azawi, S;Worden, F;Katz, S;Adkins, D;Bonomi, M;Scott, Z;Elder, J;Sonis, ST;Straube, R;Donini, O;Dusquetide: Reduction in oral mucositis associated with enduring ancillary benefits in tumor resolution and decreased mortality in head and neck cancer patientsBiotechnology reports (Amsterdam, Netherlands)286495571).Fig. 1Dusquetide Pre-Incubated with Stromal Cells inhibits the Future Growth of Co-cultured Multiple Myeloma Cells. Previously defined primary human bone marrow stromal (mesenchymal (MSC)) cells (ReachBio lot# 2221207) were plated at a concentratiFull Paper
2017Bennett, LF;Analysis of Stress Erythropoiesis: Stimulation of Toll-Like Receptors Induces Erythrophagocytosis and Activates Stress ErythropoiesisThesisAfter 24 hours, cells were washed and replated with fresh SEEM. Human bone marrow mononuclear cells were purchased from ReachBio (0300-200) and cultured in SEEM containing human insulin, human holo-transferrin, and human cytokines. Human MNCs cultureFull Paper
2017Bogeholz, JL;CD 33 in der akuten melodischen LeukämieThesisColonyGel 1101 ReachBio, Seattle, WA Steriles Wasser Helmholtzzentrum Fc-Block (FcR Blocking Reagent) Miltenyi Biotec, Bergisch GladbachFull Paper
2016Goldberg, SB;Gettinger, SN;Mahajan, A;Chiang, AC;Herbst, RS;Sznol, M;Tsiouris, AJ;Cohen, J;Vortmeyer, A;Jilaveanu, L;Yu, J;Hegde, U;Speaker, S;Madura, M;Ralabate, A;Rivera, A;Rowen, E;Gerrish, H;Yao, X;Chiang, V;Kluger, HM;Pembrolizumab for patients with melanoma or non-small-cell lung cancer and untreated brain metastases: early analysis of a non-randomised, open-label, phase 2 trialLancet Oncol.27267608In patients with NSCLC, PD-L1 staining was done by Qualtek (Goleta, CA, USA) on tissue from any disease site after the most recent systemic therapy employing previously described methods 6 using 1% staining as a cutpoint for positivityFull Paper
2016Seiwert, TY;Burtness, B;Mehra, R;Weiss, J;Berger, R;Eder, JP;Heath, K;McClanahan, T;Lunceford, J;Gause, C;Cheng, JD;Chow, LQ;Safety and clinical activity of pembrolizumab for treatment of recurrent or metastatic squamous cell carcinoma of the head and neck (KEYNOTE-012): an open-label, multicentre, phase 1b trialLancet Oncol.27247226Funding for this research was provided by Merck & Co, Inc, Kenilworth, NJ, USA. The authors thank the patients and their families and caregivers for participating in the study. The authors also thank Mark Ayers, Michael Nebozhyn, Erin Murphy, and JenFull Paper
2016Muro, K;Chung, HC;Shankaran, V;Geva, R;Catenacci, D;Gupta, S;Eder, JP;Golan, T;Le, DT;Burtness, B;McRee, AJ;Lin, CC;Pathiraja, K;Lunceford, J;Emancipator, K;Juco, J;Koshiji, M;Bang, YJ;Pembrolizumab for patients with PD-L1-positive advanced gastric cancer (KEYNOTE-012): a multicentre, open-label, phase 1b trialLancet Oncol.27157491To screen for PD-L1 expression at enrolment, archival tumour samples were assessed with a laboratory-developed prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA, USA), which included the 22C3 antibody (Merck & Co, KenilFull Paper
2016Snyder, MW;Kircher, M;Hill, AJ;Daza, RM;Shendure, J;Cell-free DNA Comprises an In Vivo Nucleosome Footprint that Informs Its Tissues-Of-OriginCell26771485... man peripheral blood plasma from healthy donors, donors with clinical diagnosis of Stage IV cancer, and donors with clinical diagnosis with autoimmune disease (Tables S1, S4 and S5) was obtained from Conversant Bio (Huntsville, Alabama, USA) or PFull Paper
2016Armand, P;Shipp, MA;Ribrag, V;Michot, JM;Zinzani, PL;Kuruvilla, J;Snyder, ES;Ricart, AD;Balakumaran, A;Rose, S;Moskowitz, CH;Programmed Death-1 Blockade With Pembrolizumab in Patients With Classical Hodgkin Lymphoma After Brentuximab Vedotin FailureJ. Clin. Oncol.27354476PD-L1 expression was determined using either fresh or archival formalin-fixed, paraffin-embedded tissue sectioned at 4 to 5 microns, with a proprietary assay developed at QualTek Molecular Laboratories (Newtown, PA) in collaboration with Merck (KenilFull Paper
2016Nanda, R;Chow, LQ;Dees, EC;Berger, R;Gupta, S;Geva, R;Pusztai, L;Pathiraja, K;Aktan, G;Cheng, JD;Karantza, V;Buisseret, L;Pembrolizumab in Patients With Advanced Triple-Negative Breast Cancer: Phase Ib KEYNOTE-012 StudyJ. Clin. Oncol.27138582Filhart and Kenneth Emancipator (Merck & Co., Kenilworth, NJ) for immunohistochemistry expertise and study support, Roger Dansey (Merck & Co.) for critical manuscript review and study support, Karl Heath (Merck & Co.) for study support, and QualTek MFull Paper
2016Imsland, F;McGowan, K;Rubin, CJ;Henegar, C;Sundström, E;Berglund, J;Schwochow, D;Gustafson, U;Imsland, P;Lindblad-Toh, K;Lindgren, G;Mikko, S;Millon, L;Wade, C;Schubert, M;Orlando, L;Penedo, MC;Barsh, GS;Andersson, L;Regulatory mutations in TBX3 disrupt asymmetric hair pigmentation that underlies Dun camouflage color in horsesNat. Genet.26691985Science for Life Laboratory Uppsala, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, USA.++Science for Life Laboratory Uppsala, Department of MedFull Paper
2016Wang, G;Lu, X;Dey, P;Deng, P;Wu, CC;Jiang, S;Fang, Z;Zhao, K;Konaparthi, R;Hua, S;Zhang, J;Li-Ning-Tapia, EM;Kapoor, A;Wu, CJ;Patel, NB;Guo, Z;Ramamoorthy, V;Tieu, TN;Heffernan, T;Zhao, D;Shang, X;Khadka, S;Hou, P;Hu, B;Jin, EJ;Yao, W;Pan, X;Ding, Z;Shi, Y;Li, L;Chang, Q;Troncoso, P;Logothetis, CJ;McArthur, MJ;Chin, L;Wang, YA;DePinho, RA;Targeting YAP-Dependent MDSC Infiltration Impairs Tumor ProgressionCancer Discov26701088... d R&D Biosystems (Minneaplois, MN). Cxcr2 antibody was obtained from Bioss and R & D Biosystems. CD45 and Ly-6G antibodies were obtained from Biolegend. Prostate tissue micrcroarray was obtained from Folio Bioscience. CHROMATIN IMMUNOPRECIPITATIFull Paper
2016Vilgelm, AE;Johnson, CA;Prasad, N;Yang, J;Chen, SC;Ayers, GD;Pawlikowski, JS;Raman, D;Sosman, JA;Kelley, M;Ecsedy, JA;Shyr, Y;Levy, SE;Richmond, A;Connecting the Dots: Therapy-Induced Senescence and a Tumor-Suppressive Immune MicroenvironmentJ. Natl. Cancer Inst.26719346Tennessee Valley Healthcare System, Department of Veterans Affairs, Nashville, TN (AEV, CAJ, JY, JSP, DR, AR); Department of Cancer Biology (AEV, AJ, JY, SCC, JSP, DR, AR), Center for Quantitative Sciences (SCC), Division of Cancer Biostatistics, DepFull Paper
2016Sun, JM;Zhou, W;Choi, YL;Choi, SJ;Kim, SE;Wang, Z;Dolled-Filhart, M;Emancipator, K;Wu, D;Weiner, R;Frisman, D;Kim, HK;Choi, YS;Shim, YM;Kim, J;Prognostic Significance of PD-L1 in Patients with Non-Small Cell Lung Cancer: A Large Cohort Study of Surgically Resected CasesJ Thorac Oncol27103510Automated staining (QualTek TekMate 500, QualTek Molecular Laboratories, Newtown, PA) included rinses with 1× wash buffer (#K8012 Envision FLEX+ kit, Dako), 10-minute proteinase K incubation (#S3020, Dako), 1× wash buffer rinse, and peroxidase blockiFull Paper
2016Wakeman, CA;Moore, JL;Noto, MJ;Zhang, Y;Singleton, MD;Prentice, BM;Gilston, BA;Doster, RS;Gaddy, JA;Chazin, WJ;Caprioli, RM;Skaar, EP;The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interactionNat Commun27301800Total RNA was harvested using a combination of LETS buffer (0.1 M LiCl; 10 mM EDTA; 10 mM Tris HCl pH 7.4; 1% SDS) and TRI Reagent as previously described61. qRT–PCR was performed using SYBR green supermix (Bio-Rad) following manufacturer’s instructiFull Paper
2016Yang, Z;Shah, K;Khodadadi-Jamayran, A;Jiang, H;Dpy30 is critical for maintaining the identity and function of adult hematopoietic stem cellsJ. Exp. Med.27647347Total RNAs and ChIP (and their corresponding input) DNAs were sent to the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology (Huntsville, AL) for sequencing, including library construction. In brief, the quality of the total RNA andFull Paper
2016Resendez, SL;Jennings, JH;Ung, RL;Namboodiri, VM;Zhou, ZC;Otis, JM;Nomura, H;McHenry, JA;Kosyk, O;Stuber, GD;Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lensesNat Protoc26914316Dust-off compressed gas (Falcon Safety Products, DPSXL) Gelfoam absorbable sponge (Pfizer Injectables, cat. no. 00009-0342-01) 12-well culture plates (Falcon, cat. no. 353043) Skull screws (Glass and Watch Screws, 900 piece set, JT69900) 1/16-incFull Paper
2016Amendola, LM;Jarvik, GP;Leo, MC;McLaughlin, HM;Akkari, Y;Amaral, MD;Berg, JS;Biswas, S;Bowling, KM;Conlin, LK;Cooper, GM;Dorschner, MO;Dulik, MC;Ghazani, AA;Ghosh, R;Green, RC;Hart, R;Horton, C;Johnston, JJ;Lebo, MS;Milosavljevic, A;Ou, J;Pak, CM;Patel, RY;Punj, S;Richards, CS;Salama, J;Strande, NT;Yang, Y;Plon, SE;Biesecker, LG;Rehm, HL;Performance of ACMG-AMP Variant-Interpretation Guidelines among Nine Laboratories in the Clinical Sequencing Exploratory Research ConsortiumAm. J. Hum. Genet.27181684Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA.++Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA. Electronic address: pair@u.washington.eduFull Paper
2016Green, RC;Goddard, KAB;Jarvik, GP;Amendola, LM;Appelbaum, PS;Berg, JS;Bernhardt, BA;Biesecker, LG;Biswas, S;Blout, CL;Bowling, KM;Brothers, KB;Burke, W;Caga-Anan, CF;Chinnaiyan, AM;Chung, WK;Clayton, EW;Cooper, GM;East, K;Evans, JP;Fullerton, SM;Garraway, LA;Garrett, JR;Gray, SW;Henderson, GE;Hindorff, LA;Holm, IA;Lewis, MH;Hutter, CM;Janne, PA;Joffe, S;Kaufman, D;Knoppers, BM;Koenig, BA;Krantz, ID;Manolio, TA;McCullough, L;McEwen, J;McGuire, A;Muzny, D;Myers, RM;Nickerson, DA;Ou, J;Parsons, DW;Petersen, GM;Plon, SE;Rehm, HL;Roberts, JS;Robinson, D;Salama, JS;Scollon, S;Sharp, RR;Shirts, B;Spinner, NB;Tabor, HK;Tarczy-Hornoch, P;Veenstra, DL;Wagle, N;Weck, K;Wilfond, BS;Wilhelmsen, K;Wolf, SM;Wynn, J;Yu, JH;, ;Clinical Sequencing Exploratory Research Consortium: Accelerating Evidence-Based Practice of Genomic MedicineAm. J. Hum. Genet.27181682Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Medical School, Boston, MA 02115, USA; Partners Personalized Medicine, Boston, MAFull Paper
2016Lieberman, LA;Zeng, W;Singh, C;Wang, W;Otipoby, KL;Loh, C;Plavina, T;Gorelik, L;Ransohoff, RM;Cahir-McFarland, E;CD62L is not a reliable biomarker for predicting PML risk in natalizumab-treated R-MS patientsNeurology26718566Thirty-four frozen PBMC samples were purchased from Conversant Bio (Huntsville, AL) to test the effects of other medical conditions on the % CD62L determination. Twenty-one matched healthy donor samples were collected. Eight samples were collected frFull Paper
2016Hu, X;Liu, X;Moisan, J;Wang, Y;Lesch, CA;Spooner, C;Morgan, RW;Zawidzka, EM;Mertz, D;Bousley, D;Majchrzak, K;Kryczek, I;Taylor, C;Van Huis, C;Skalitzky, D;Hurd, A;Aicher, TD;Toogood, PL;Glick, GD;Paulos, CM;Zou, W;Carter, LL;Synthetic RORγ agonists regulate multiple pathways to enhance antitumor immunityOncoimmunology28123897... ion. After 5 days, cytokine levels in the media were determined using a luminex panel (R&D Systems). Cells were collected for flow cytometry analysis. PBMCs from cancer patients were purchased from Conversant Bio and activated with anti-CD3/28 bFull Paper
2016Cataisson, C;Michalowski, AM;Shibuya, K;Ryscavage, A;Klosterman, M;Wright, L;Dubois, W;Liu, F;Zhuang, A;Rodrigues, KB;Hoover, S;Dwyer, J;Simpson, MR;Merlino, G;Yuspa, SH;MET signaling in keratinocytes activates EGFR and initiates squamous carcinogenesisSci Signal27330189This procedure was performed by a commercial vendor (Phylogeny, Inc.) according to the published method (88). FFPE TMAs from US Biomax were deparaffinized for 5 minutes in xylene, immersed in 100% ethanol for 5 minutes then air-dried. Treatment was wFull Paper
2016Bryant, SA;Herdy, JR;Amemiya, CT;Smith, JJ;Characterization of Somatically-Eliminated Genes During Development of the Sea Lamprey (Petromyzon marinus)Mol. Biol. Evol.27288344Total RNA was isolated from lamprey embryos using Trizol extraction. RNA quality was assessed on the Agilent 2100 Bioanalyzer (Agilent Technologies) and samples with RNA Integrity Number (RIN) 8 were sent to the HudsonAlpha Genomic Services Lab (HudsFull Paper
2016Jiang, L;Huang, J;Higgs, BW;Hu, Z;Xiao, Z;Yao, X;Conley, S;Zhong, H;Liu, Z;Brohawn, P;Shen, D;Wu, S;Ge, X;Jiang, Y;Zhao, Y;Lou, Y;Morehouse, C;Zhu, W;Sebastian, Y;Czapiga, M;Oganesyan, V;Fu, H;Niu, Y;Zhang, W;Streicher, K;Tice, D;Zhao, H;Zhu, M;Xu, L;Herbst, R;Su, X;Gu, Y;Li, S;Huang, L;Gu, J;Han, B;Jallal, B;Shen, H;Yao, Y;Genomic Landscape Survey Identifies SRSF1 as a Key Oncodriver in Small Cell Lung CancerPLoS Genet.27093186... s >70%; the tumor content in each NAT was< 3%. The Caucasian SCLC patient cohort consisted of 25 FFPE lung tumor tissue specimens with matched normal adjacent tissue pairs, which were purchased from Conversant Biologics, Inc (Huntsville, AL) (S1Full Paper
2016Oksenberg, D;Dufu, K;Patel, MP;Chuang, C;Li, Z;Xu, Q;Silva-Garcia, A;Zhou, C;Hutchaleelaha, A;Patskovska, L;Patskovsky, Y;Almo, SC;Sinha, U;Metcalf, BW;Archer, DR;GBT440 increases haemoglobin oxygen affinity, reduces sickling and prolongs RBC half-life in a murine model of sickle cell diseaseBr. J. Haematol.27378309USA (IRB 88‐034)]. Blood from healthy donors was obtained from the Stanford Blood Center (Stanford, CA, USA) and umbilical cord blood was obtained from Conversant Bio, Inc. (Huntsville, AL, USA). The anti‐coagulant wasFull Paper
2016Kirby, MK;Ramaker, RC;Gertz, J;Davis, NS;Johnston, BE;Oliver, PG;Sexton, KC;Greeno, EW;Christein, JD;Heslin, MJ;Posey, JA;Grizzle, WE;Vickers, SM;Buchsbaum, DJ;Cooper, SJ;Myers, RM;RNA sequencing of pancreatic adenocarcinoma tumors yields novel expression patterns associated with long-term survival and reveals a role for ANGPTL4Mol Oncol27282075... wling for critical reading of the manuscript. We thank Dr. Nick Cochran for packaging the _ANGPTL4_ lentiviral vectors. We thank Dr. Shawn Levy, Braden Boone, Angela Jones, and all the members of the HudsonAlpha Genomic Services Lab for providingFull Paper
2016O'Shannessy, DJ;Smith, MF;Somers, EB;Jackson, SM;Albone, E;Tomkowicz, B;Cheng, X;Park, Y;Fernando, D;Milinichik, A;Kline, B;Fulton, R;Oberoi, P;Nicolaides, NC;Novel antibody probes for the characterization of endosialin/TEM-1Oncotarget27494870... btained from various commercial vendors with Institutional Review Board approvals and patient consent and were collected between 2009 and 2011 (Bioreclamation, Westbury, NY; Indivumed, Lewisburg, PA; Folio Biosciences, Columbus, OH; Discovery LifFull Paper
2016Ladd, B;Mazzola, AM;Bihani, T;Lai, Z;Bradford, J;Collins, M;Barry, E;Goeppert, AU;Weir, HM;Hearne, K;Renshaw, JG;Mohseni, M;Hurt, E;Jalla, S;Bao, H;Hollingsworth, R;Reimer, C;Zinda, M;Fawell, S;D'Cruz, CM;Effective combination therapies in preclinical endocrine resistant breast cancer models harboring ER mutationsOncotarget27472462... ired ER mutations. MATERIALS AND METHODS Generation of the CTX model/efficacy studies Blood samples from MBC patients, consented according to the Human Biological Samples Policy, were purchased from Conversant Biologics. Due to patient confidentFull Paper
2016Bradford, JR;Wappett, M;Beran, G;Logie, A;Delpuech, O;Brown, H;Boros, J;Camp, NJ;McEwen, R;Mazzola, AM;D'Cruz, C;Barry, ST;Whole transcriptome profiling of patient-derived xenograft models as a tool to identify both tumor and stromal specific biomarkersOncotarget26980748... subcutaneously into either NSG or CB17 SCID mice. The HBXF-079 sample was implanted orthotopically. The CTC-174 model was developed from circulating tumor cells in patient blood samples procured from Conversant Biologics (Huntsville, AL) and consFull Paper
2016Malek, E;Driscoll, JJ;High throughput chemical library screening identifies a novel p110-δ inhibitor that potentiates the anti-myeloma effect of bortezomibOncotarget... om Selleck Chemicals (Houston, TX). Human bone marrow stromal cell line HS-5 (ATCC) was cultured in DMEM. Patient bone marrow aspirates were obtained after Institutional Review Board approval or from ConversantBio (Huntsville, AL) and CD138+ cellFull Paper
2016Aeffner, F;Martin, NT;Peljto, M;Black, JC;Major, JK;Jangani, M;Ports, MO;Krueger, JS;Young, GD;Quantitative assessment of pancreatic cancer precursor lesions in IHC-stained tissue with a tissue image analysis platformLab. Invest.27775692tissue was evaluated. Materials and methods. Human PDAC Tissue. IRB-approved, formalin-fixed tumor samples from pancreatic cancer patients were provided by Conversant Bio (Huntsville, AL, USA). Animals. Kras LSL-G12DFull Paper
2016Haskett, S;Ding, J;Zhang, W;Thai, A;Cullen, P;Xu, S;Petersen, B;Kuznetsov, G;Jandreski, L;Hamann, S;Reynolds, TL;Allaire, N;Zheng, TS;Mingueneau, M;Identification of Novel CD4+ T Cell Subsets in the Target Tissue of Sjögren's Syndrome and Their Differential Regulation by the Lymphotoxin/LIGHT Signaling AxisJ. Immunol.27815440Blocks of formalin-fixed tissue biopsies were acquired from Folio Biosciences (Powell, OH). Salivary tissues included submandibular glands (n = 2), parotid glands (n = 1), and labial glands (n = 3) from Sjo¨gren’s patients. Diagnoses were consistentFull Paper
2016Yoder, KE;Bundschuh, R;Host Double Strand Break Repair Generates HIV-1 Strains Resistant to CRISPR/Cas9Sci Rep27404981HIV-1 infections were continued for 21 days in 4 replicates per cell type and virus strain. The virus supernatant was passaged to uninfected CRISPR cells. Infection was visually confirmed by the presence of syncytia. Two weeks later the genomic DNA wFull Paper
2016Dolled-Filhart, M;Locke, D;Murphy, T;Lynch, F;Yearley, JH;Frisman, D;Pierce, R;Weiner, R;Wu, D;Emancipator, K;Development of a Prototype Immunohistochemistry Assay to Measure Programmed Death Ligand-1 Expression in Tumor TissueArch. Pathol. Lab. Med.27788043Merck & Co, Inc, Kenilworth, New Jersey; the Departments of Early Clinical & Translational Research, Clinical Histochemistry (Dr Locke), Research and Clinical Client Services (Dr Murphy), and Operations and Client Services (Dr Lynch), QualTek MoleculFull Paper
2016Dolled-Filhart, M;Roach, C;Toland, G;Stanforth, D;Jansson, M;Lubiniecki, GM;Ponto, G;Emancipator, K;Development of a Companion Diagnostic for Pembrolizumab in Non-Small Cell Lung Cancer Using Immunohistochemistry for Programmed Death Ligand-1Arch. Pathol. Lab. Med.27552095The prototype PD-L1 IHC assay was used to determine enrollment of patients in KEYNOTE-001 and was performed at a single laboratory site at QualTek (Goleta, California), which is a College of American Pathologists/Clinical Laboratory Improvement AmendFull Paper
2016Durning, SP;Flanagan-Steet, H;Prasad, N;Wells, L;O-Linked β-N-acetylglucosamine (O-GlcNAc) Acts as a Glucose Sensor to Epigenetically Regulate the Insulin Gene in Pancreatic Beta CellsJ. Biol. Chem.26598517samples after the previously described 1-h incubation. Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL) for RNA-Seq library prep and sequencing. Briefly, the concentration and integrity of theFull Paper
2016VonDran, M;Thomas, JA;Freund, MP;Ritsick, M;Orr, M;Kaye, WE;Bakker, A;Knight, P;The National Disease Research Interchange and Collaborators on: What Are the Major Hurdles to the Recovery of Human Tissue to Advance Research?Biopreserv Biobank27845557The Foundation has collected more than 100 cutaneous neurofibromas along with patient-reported NF medical histories. Both tissue and data are stored at Precision Bioservices (Frederick, MD). Full “omic” characterization of 50 samples was outsourced tFull Paper
2016Chisamore, MJ;Gentile, MA;Dillon, GM;Baran, M;Gambone, C;Riley, S;Schmidt, A;Flores, O;Wilkinson, H;Alves, SE;A novel selective androgen receptor modulator (SARM) MK-4541 exerts anti-androgenic activity in the prostate cancer xenograft R-3327G and anabolic activity on skeletal muscle mass & function in castrated miceJ. Steroid Biochem. Mol. Biol.27106747Excised tumor samples were immediately fixed with 10% buffered formalin, and sent to QualTek (Santa Barbara, CA) where tumors were embedded in paraffin, 4-μm thick sections were mounted on charged slides, dried at 60 °C for one hour, and deparaffinzeFull Paper
2016Qadota, H;Mayans, O;Matsunaga, Y;McMurry, JL;Wilson, KJ;Kwon, GE;Stanford, R;Deehan, K;Tinley, TL;Ngwa, VM;Benian, GM;The SH3 domain of UNC-89 (obscurin) interacts with paramyosin, a coiled-coil protein, in Caenorhabditis elegans muscleMol. Biol. Cell27009202Genomic DNA from each strain was prepared and provided to the Emory Integrated Genomics Core (EIGC) for Illumina sequencing. The Genomic Services Laboratory at the HudsonAlpha Institute for Biotechnology (Huntsville, AL) performed quality control andFull Paper
2016Slamecka, J;Salimova, L;McClellan, S;van Kelle, M;Kehl, D;Laurini, J;Cinelli, P;Owen, L;Hoerstrup, SP;Weber, B;Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditionsCell Cycle26654216RNA was extracted from iPSC cultures using RNeasy Plus Mini kit (74134, Qiagen, https://www.qiagen.com/us/shop/sample-technologies/rna-sample-technologies/total-rna/rneasy-plus-micro-and-mini-kits). Whole genome expression analysis was performed by tFull Paper
2016Aycock, KI;Campbell, RL;Lynch, FC;Manning, KB;Craven, BA;The Importance of Hemorheology and Patient Anatomy on the Hemodynamics in the Inferior Vena CavaAnn Biomed Eng27272211Full Paper
2016Rahman, KM;Camp, ME;Prasad, N;McNeel, AK;Levy, SE;Bartol, FF;Bagnell, CA;Age and Nursing Affect the Neonatal Porcine Uterine TranscriptomeBiol. Reprod.26632611United States Department of Agriculture, Agriculture Research Service, Clay Center, Nebraska Department of Animal Sciences, Endocrinology and Animal Biosciences Program, Rutgers University, New Brunswick, New Jersey.++Department of Animal Sciences, EFull Paper
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2016Sorensen, SF;Zhou, W;Dolled-Filhart, M;Georgsen, JB;Wang, Z;Emancipator, K;Wu, D;Busch-Sørensen, M;Meldgaard, P;Hager, H;PD-L1 Expression and Survival among Patients with Advanced Non-Small Cell Lung Cancer Treated with ChemotherapyTransl Oncol26947883gen retrieval, along with a 10-minute proteinase K incubation (#S3020, Dako) following a 1 × wash buffer rinse (#K8012 Envision FLEX + kit, Dako) as part of automated staining. Automated staining (QualTek TekMate 500, Newtown, PA) was followed by perFull Paper
2016Zea, L;Prasad, N;Levy, SE;Stodieck, L;Jones, A;Shrestha, S;Klaus, D;A Molecular Genetic Basis Explaining Altered Bacterial Behavior in SpacePLoS ONE27806055BioServe Space Technologies, Aerospace Engineering Sciences Dept., University of Colorado, Boulder, CO, United States of America.++Genomic Services Laboratory, HudsonAlpha Institute for Biotechnology, Huntsville, AL, United States of America.++GenomiFull Paper
2016McCoy, CR;Rana, S;Stringfellow, SA;Day, JJ;Wyss, JM;Clinton, SM;Kerman, IA;Neonatal maternal separation stress elicits lasting DNA methylation changes in the hippocampus of stress-reactive Wistar Kyoto ratsEur. J. Neurosci.27643783; Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) for next‐generation sequencing using NEBNext reagents (New England Biolabs, Ipswich, MA) according to manufacturer's recommendationsFull Paper
2016Beck, BH;Fuller, SA;Li, C;Green, BW;Zhao, H;Rawles, SD;Webster, CD;Peatman, E;Hepatic transcriptomic and metabolic responses of hybrid striped bass (Morone saxatilis×Morone chrysops) to acute and chronic hypoxic insultComp. Biochem. Physiol. Part D Genomics Proteomics26851735treatment) were used for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25Full Paper
2016Póvoa, TF;Oliveira, ER;Basílio-de-Oliveira, CA;Nuovo, GJ;Chagas, VL;Salomão, NG;de Barcelos Alves, AM;Mota, EM;Paes, MV;Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing CellsPLoS ONE28006034Janeiro, Brazil. ⨯. Gerard J. Nuovo, Affiliations Ohio State University Comprehensive Cancer Center, Columbus, Ohio, United States of America, Phylogeny Inc, Powell, Ohio, United States of America. ⨯. Vera LA Chagas, AffiliationFull Paper
2016Stavropoulos, SW;Chen, JX;Sing, RF;Elmasri, F;Silver, MJ;Powell, A;Lynch, FC;Abdel Aal, AK;Lansky, A;Muhs, BE;, ;Analysis of the Final DENALI Trial Data: A Prospective, Multicenter Study of the Denali Inferior Vena Cava FilterJ Vasc Interv Radiol27569678Full Paper
2016Gardet, A;Chou, WC;Reynolds, TL;Velez, DB;Fu, K;Czerkowicz, JM;Bajko, J;Ranger, AM;Allaire, N;Kerns, HM;Ryan, S;Legault, HM;Dunstan, RW;Lafyatis, R;Lukashev, M;Viney, JL;Browning, JL;Rabah, D;Pristane-Accelerated Autoimmune Disease in (SWR X NZB) F1 Mice Leads to Prominent Tubulointerstitial Inflammation and Human Lupus Nephritis-Like FibrosisPLoS ONE27760209AZ, USA). Needle biopsies from 5 patients with LN (Class II, 1 patient; Class III, 1 patient; Class IV, 1 patient; Class IV/V, 2 patients) were obtained from Folio Biosciences (Powell, OH) and stained using the same methods. ForFull Paper
2016Shearstone, JR;Golonzhka, O;Chonkar, A;Tamang, D;van Duzer, JH;Jones, SS;Jarpe, MB;Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2PLoS ONE27073918CS2 differentiation media is same as CS2 expansion media, except 3 U/mL EPO. Peripheral blood from patients homozygous for the sickle cell mutation (Conversant Bio) were collected in heparinized tubes and shipped overnight on cool packsFull Paper
2016Shimizu, T;Seto, T;Hirai, F;Takenoyama, M;Nosaki, K;Tsurutani, J;Kaneda, H;Iwasa, T;Kawakami, H;Noguchi, K;Shimamoto, T;Nakagawa, K;Phase 1 study of pembrolizumab (MK-3475; anti-PD-1 monoclonal antibody) in Japanese patients with advanced solid tumorsInvest New Drugs27000274on the confirmation test. PD-L1 expression. PD-L1 expression was measured using immunohistochemistry performed on formalin-fixed, paraffin-embedded tissue sections at QualTek Clinical Laboratories. PD-L1 positivity was ;or postdose sample was poFull Paper
2016Meaburn, KJ;Agunloye, O;Devine, M;Leshner, M;Roloff, GW;True, LD;Misteli, T;Tissue-of-origin-specific gene repositioning in breast and prostate cancerHistochem. Cell Biol.26791532Tissue sections and tissue microarrays (TMAs) were purchased from US Biomax Inc, Imgenex Corporation, Folio Bioscience and Biochain Institute or were acquired from the University of Washington under the guidelines and approval of the Institutional ReFull Paper
2016Quadrini, KJ;Hegelund, AC;Cortes, KE;Xue, C;Kennelly, SM;Ji, H;Högerkorp, CM;Mc Closkey, TW;Validation of a flow cytometry-based assay to assess C5aR receptor occupancy on neutrophils and monocytes for use in drug developmentCytometry B Clin Cytom26084468a specific Disease Activity Score 28‐joint counts (DAS28) criteria of C‐reactive protein (CRP) ≥ 3.2 and ≥ 2 swollen joints 10, 11 was drawn into K 2 ‐EDTA phlebotomy tubes and stored at 2-8°C. RA patient samples were obtained from Conversant BiologiFull Paper
2016Gebauer, A;Schmidt, S;Hoffmann, W;Status and perspective of lab-on-a-chip systems for common diseases: a systematic review from 2003 to 2013Per Med29749869Diagnosis, monitoring Laboratory Patients with advanced-stage breast cancer 73/73 patients 5/5 negative control Conversant Bio (USA), bio options (USA) ? 61 Prospective experimental study Diagnosis, monitoring ? Patients with metastatic lung (n = 55)Full Paper
2016Shelton, D;Quinlan, A;Rapid, Sensitive Cancer Biomarker Screening in cfDNAGenetic Engineering & Biotechnology NewsUntested plasma samples from patients who had previously been classified as KRAS mutation positive or negative by FFPE genotyping were purchased from Conversant Bio and ProMedDx. Figure 3. Multiplex detection of KRAS mutations in patient cell-free DNFull Paper
2016Justice, JL;Weese, DA;Santos, SR;Phylogenetic utility, and variability in structure and content, of complete mitochondrial genomes among genetic lineages of the Hawaiian anchialine shrimp Halocaridina rubra Holthuis 1963 (Atyidae:Decapoda)Mitochondrial DNA A DNA Mapp Seq Anal26061341The Atyidae are caridean shrimp possessing hair-like setae on their claws and are important contributors to ecological services in tropical and temperate fresh and brackish water ecosystems. Complete mitochondrial genomes have only been reported fromFull Paper
2016Li, S;Garrett-Bakelman, FE;Chung, SS;Sanders, MA;Hricik, T;Rapaport, F;Patel, J;Dillon, R;Vijay, P;Brown, AL;Perl, AE;Cannon, J;Bullinger, L;Luger, S;Becker, M;Lewis, ID;To, LB;Delwel, R;Löwenberg, B;Döhner, H;Döhner, K;Guzman, ML;Hassane, DC;Roboz, GJ;Grimwade, D;Valk, PJ;D'Andrea, RJ;Carroll, M;Park, CY;Neuberg, D;Levine, R;Melnick, AM;Mason, CE;Distinct evolution and dynamics of epigenetic and genetic heterogeneity in acute myeloid leukemiaNature medicine27322744rough AML_101, DNA and RNA were extracted from mononuclear cell layers using standard techniques. Normal bone marrow controls (NBM: CD34+ mononuclear cells; 7 males and 7 females) were purchased from AllCells (Alameda, CA, USA; n = 5) or isolated usiFull Paper
2016Dever, DP;Bak, RO;Reinisch, A;Camarena, J;Washington, G;Nicolas, CE;Pavel-Dinu, M;Saxena, N;Wilkens, AB;Mantri, S;Uchida, N;Hendel, A;Narla, A;Majeti, R;Weinberg, KI;Porteus, MH;CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cellsNature27820943blood were purchased from AllCells and thawed according to manufacturer's instructions. CD34 + HSPCs from cord blood were either purchased frozen from AllCells or acquired from SaveFull Paper
2016Briney, B;Sok, D;Jardine, JG;Kulp, DW;Skog, P;Menis, S;Jacak, R;Kalyuzhniy, O;de Val, N;Sesterhenn, F;Le, KM;Ramos, A;Jones, M;Saye-Francisco, KL;Blane, TR;Spencer, S;Georgeson, E;Hu, X;Ozorowski, G;Adachi, Y;Kubitz, M;Sarkar, A;Wilson, IA;Ward, AB;Nemazee, D;Burton, DR;Schief, WR;Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing AntibodiesCell27610570pathogen free environment. Healthy, HIV-Negative Human PBMCs Leukopaks from two heathy, HIV-negative individuals (a 28 year-old female and a 33 year-old male) were obtained from a commercial vendor (AllCells) using a protocol approved by the InstituFull Paper
2016Corces, MR;Buenrostro, JD;Wu, B;Greenside, PG;Chan, SM;Koenig, JL;Snyder, MP;Pritchard, JK;Kundaje, A;Greenleaf, WJ;Majeti, R;Chang, HY;Lineage-specific and single-cell chromatin accessibility charts human hematopoiesis and leukemia evolutionNature genetics27526324Normal donor human bone marrow and peripheral blood cells were obtained fresh from AllCells or the Stanford Blood Center. All normal blood cell populations were sorted fresh. SaveFull Paper
2016Kim, JH;Hu, Y;Yongqing, T;Kim, J;Hughes, VA;Le Nours, J;Marquez, EA;Purcell, AW;Wan, Q;Sugita, M;Rossjohn, J;Winau, F;CD1a on Langerhans cells controls inflammatory skin diseaseNature immunology27548435lysis by ELISA. K562 cells were routinely tested for mycoplasma contamination, and CD1a expression of transfectants was confirmed before use by flow cytometry. PBMCs were obtained from ConversantBio, ALLCELLS, and the Kraft Family Blood Donor CenterFull Paper
2016Locci, M;Wu, JE;Arumemi, F;Mikulski, Z;Dahlberg, C;Miller, AT;Crotty, S;Activin A programs the differentiation of human TFH cellsNature immunology27376469Leukapheresis sample from a healthy donor was obtained from AllCells, Inc., after a prescreening of multiple donors. Fresh whole blood samples from healthy donors were obtained SaveFull Paper
2016Wang, X;Haylock, D;Hu, CS;Kowalczyk, W;Jiang, T;Qiu, J;Mosoyan, G;He, W;Marshall, N;Mascarenhas, J;Tarasova, A;Brody, J;Winkler, D;Hoffman, R;A thrombopoietin receptor antagonist is capable of depleting myelofibrosis hematopoietic stem and progenitor cellsBlood27114459Flow Cytometer (Becton Dickinson, Franklin Lakes, NJ) were used in each experiment. BM CD34+ cells from normal individuals were purchased from AllCells (Alameda, CA). The JAK2, CALR, and MPL mutational status1,22-24 of each patient is shown in TableFull Paper
2016Zhang, Y;Quarmyne, M;Himburg, H;Yan, X;McBride, W;Jung, M;Chute, J;A Small Molecule Inhibitor of Protein Tyrosine Phosphatase-Sigma (PTPσ) Promotes Hematopoietic Stem Cell (HSC) RegenerationBloodOur laboratory previously discovered that pleiotrophin, a paracrine growth factor secreted by the BM perivascular and endothelial cells, promoted HSC self-renewal and regeneration SaveFull Paper
2016Iancu-Rubin, C;Fong, H;Mosoyan, G;Patel, A;Sabado, R;Marcia, M;Moshier, E;Ozbek, U;Keyzner, A;Bhardwaj, N;McNiece, I;Tong, J;Hoffman, R;Preclinical Development of a Cord Blood (CB)-Derived Hematopoietic Stem Cell (HSC) Product for Allogeneic Transplantation in Patients with Hematological MalignanciesBloodDivision of Hematology and Medical Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY++AllCells, LLC, Alameda, CA++Division of Hematology and Medical Oncology, The Tisch Cancer Institute, Icahn School of MedicFull Paper
2016Hoff, F;Hu, C;Qiu, Y;Yoo, S;Qutub, A;Horton, T;Kornblau, S;Recurrent Patterns of Protein Expression Signatures in Pediatric Acute Lymphoblastic Leukemia: Recognition and Therapeutic GuidanceBloodreverse-phase protein arrays (RPPA) methodology was performed on 73 samples from pediatric patients with ALL, 10 cryopreserved CD34 + normal bone marrow samples (AllCells) SaveFull Paper
2016Yoshioka, S;Miura, Y;Iwasa, M;Fujishiro, A;Sugino, N;Fujii, S;Nakagawa, Y;Sato, A;Yokota, A;Hirai, H;Ichinohe, T;Takaori, A;Maekawa, T;Late Adherent Subpopulation in Umbilical Cord Blood Has the Same Characteristics and Hematopoiesis-Supporting Capacity As Mesenchymal Stromal/Stem CellsBloodMesenchymal stromal/stem cells (MSCs) are a major source of cell for cell therapy. MSCs derived from bone marrow (BMMSCs) have been mostly used in clinical applications. BMMSCs can be easily isolated as a cell population that adheres to plastic cultuFull Paper
2016Merkel, P;Giclas, H;Gibula, A;Andersen, G;Knight, V;Lin, J;Castro, G;Green, J;Stassinopoulos, A;Reinvestigation of the T-Cell Inactivation Achieved for the Prevention of TA GV HD By 2500 Gy of Gamma Irradiation, a Comparison with Pathogen ReductionBloodBackground: Transfusion Associated Graft vs. Host Disease (TA GVHD) results in high morbidity and mortality, caused by contaminating T-cells present in transfused blood products. Gamma irradiation (GI) of platelet components (PC) with a dose of 2500Full Paper
2016Kanter, J;Walters, M;Hsieh, M;Krishnamurti, L;Kwiatkowski, J;Kamble, R;von Kalle, C;Kuypers, F;Cavazzana, M;Leboulch, P;Joseney-Antoine, M;Asmal, M;Thompson, A;Tisdale, J;Interim Results from a Phase 1/2 Clinical Study of Lentiglobin Gene Therapy for Severe Sickle Cell DiseaseBloodβ-globin gene transfer into hematopoietic stem cells (HSCs) has the potential to reduce or eliminate the symptoms and long-term complications of severe sickle cell disease (SCD). LentiGlobin Drug Product (DP) is a gene therapy product containing autoFull Paper
2016Thompson, A;Kwiatkowski, J;Rasko, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;von Kalle, C;Kletzel, M;Leboulch, P;Vichinsky, E;Petrusich, A;Asmal, M;Walters, M;Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia: Update from the Northstar Hgb-204 Phase 1/2 Clinical StudyBloodBACKGROUND Allogeneic hematopoietic stem cell (HSC) transplant is potentially curative for patients with β-thalassemia major or, as more broadly defined, transfusion dependent β-thalassemia (TDT). However, HSC transplant is generally rFull Paper
2016Crews, LA;Balaian, L;Delos Santos, NP;Leu, HS;Court, AC;Lazzari, E;Sadarangani, A;Zipeto, MA;La Clair, JJ;Villa, R;Kulidjian, A;Storb, R;Morris, SR;Ball, ED;Burkart, MD;Jamieson, CHM;RNA Splicing Modulation Selectively Impairs Leukemia Stem Cell Maintenance in Secondary Human AMLCell stem cell27570067Age-related human hematopoietic stem cell (HSC) exhaustion and myeloid-lineage skewing promote oncogenic transformation of hematopoietic progenitor cells into therapy-resistant SaveFull Paper
2016Song, C;Zhang, Y;Li, C;Chen, G;Kang, X;Wang, Q;Enhanced Nanodrug Delivery to Solid Tumors Based on a Tumor Vasculature-Targeted StrategyAdvanced Functional MaterialsCell Culture: Human malignantglioma U87-MG cells were obtained from The Cell Bank of Type Culture Collection of Chinese Academy of Sciences and HUVECs were purchased from Allcells (China). U87-MG cells were cultured in low-glucose Dulbecco's modifiedFull Paper
2016Sherbenou, DW;Aftab, BT;Su, Y;Behrens, CR;Wiita, A;Logan, AC;Acosta-Alvear, D;Hann, BC;Walter, P;Shuman, MA;Wu, X;Atkinson, JP;Wolf, JL;Martin, TG;Liu, B;Antibody-drug conjugate targeting CD46 eliminates multiple myeloma cellsThe Journal of clinical investigation27841764bead columns (Stem Cell Technologies). Primary cells were cultured in RPMI1640 media with 100 U/ml penicillin, 100 μg/ml streptomycin, 10% FBS, and 2 ng/ml IL-6. BM and PB controls from normal donors were purchased from AllCellsFull Paper
2016Jiang, X;Hu, C;Arnovitz, S;Bugno, J;Yu, M;Zuo, Z;Chen, P;Huang, H;Ulrich, B;Gurbuxani, S;Weng, H;Strong, J;Wang, Y;Li, Y;Salat, J;Li, S;Elkahloun, AG;Yang, Y;Neilly, MB;Larson, RA;Le Beau, MM;Herold, T;Bohlander, SK;Liu, PP;Zhang, J;Li, Z;He, C;Jin, J;Hong, S;Chen, J;miR-22 has a potent anti-tumour role with therapeutic potential in acute myeloid leukaemiaNature communications27116251(Axis-Shield, Oslo, Norway) according to the manufacturer’s manual. Human normal hematopoietic control cell samples The MNC normal control samples were isolated from normal BM cells purchased from AllCells, LLC (Emeryville, CA) by use of NycoPrep 1.Full Paper
2016van Overbeek, M;Capurso, D;Carter, MM;Thompson, MS;Frias, E;Russ, C;Reece-Hoyes, JS;Nye, C;Gradia, S;Vidal, B;Zheng, J;Hoffman, GR;Fuller, CK;May, AP;DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated BreaksMolecular cell27499295Mobilized peripheral blood HSCs (AllCells # mPB018F) were plated at 1.5 × 105/ml in DMEM/F12 Glutamax supplemented with 100× MNEAA solution, 1,000× 2-mercaptoethanol, 100× StemSpan CC100, 1,000× TPO, and 10,000× GM-CSFFull Paper
2016Wang, J;DeClercq, JJ;Hayward, SB;Li, PW;Shivak, DA;Gregory, PD;Lee, G;Holmes, MC;Highly efficient homology-driven genome editing in human T cells by combining zinc-finger nuclease mRNA and AAV6 donor deliveryNucleic acids research26527725Fresh purified CD8 + and CD4 + T cells were purchased from AllCells.com and maintained in X-VIVO 15 (Lonza, Basel, Switzerland) supplemented with 10% FBS, 2 mM l-glutamine, 1 SaveFull Paper
2016Beard, RS;Yang, X;Meegan, JE;Overstreet, JW;Yang, CG;Elliott, JA;Reynolds, JJ;Cha, BJ;Pivetti, CD;Mitchell, DA;Wu, MH;Deschenes, RJ;Yuan, SY;Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndromeNature communications27653213Full Paper
2016Zhao, Y;Wang, A;Zou, Y;Su, N;Loscalzo, J;Yang, Y;In vivo monitoring of cellular energy metabolism using SoNar, a highly responsive sensor for NAD(+)/NADH redox stateNature protocols27362337Cells of interest: we have used the following: NCI-H1299 non-small-cell lung cancer (ATCC, cat. no. CRL-5803), primary mouse hepatocytes (freshly isolated), primary mouse renal tubular epithelial cells (AllCells, cat. no. MRTEC-001F), primary mouse aFull Paper
2016Li, L;Qi, X;Sun, W;Abdel-Azim, H;Lou, S;Zhu, H;Prasadarao, NV;Zhou, A;Shimada, H;Shudo, K;Kim, YM;Khazal, S;He, Q;Warburton, D;Wu, L;Am80-GCSF synergizes myeloid expansion and differentiation to generate functional neutrophils that reduce neutropenia-associated infection and mortalityEMBO molecular medicine27737899opoietin. Primary cells were split every 3 days with equal portions of fresh MM‐HSCCM. Normal primary human hematopoietic precursors and cell culture Human umbilical cord blood CD34+ precursors (AllCells, Emeryville, CA) were expanded, as describedFull Paper
2016Sun, H;Lin, DC;Cao, Q;Guo, X;Marijon, H;Zhao, Z;Gery, S;Xu, L;Yang, H;Pang, B;Lee, VK;Lim, HJ;Doan, N;Said, JW;Chu, P;Mayakonda, A;Thomas, T;Forscher, C;Baloglu, E;Shacham, S;Rajalingam, R;Koeffler, HP;CRM1 Inhibition Promotes Cytotoxicity in Ewing Sarcoma Cells by Repressing EWS-FLI1-Dependent IGF-1 SignalingCancer research26956669IGFBP3 siRNA lentiviral plasmid (i010368) and EWS-FLI siRNAs (ABM Inc.); Matrigel anti-rabbit IgG and anti-mouse conjugated HRP antibodies (BD Biosciences). siRNA pools targeting DDIT3 and p53 were purchased from Dharmacon. Bone marrow cells from norFull Paper
2016Prabhala, RH;Fulciniti, M;Pelluru, D;Rashid, N;Nigroiu, A;Nanjappa, P;Pai, C;Lee, S;Prabhala, NS;Bandi, RL;Smith, R;Lazo-Kallanian, SB;Valet, S;Raje, N;Gold, JS;Richardson, PG;Daley, JF;Anderson, KC;Ettenberg, SA;Di Padova, F;Munshi, NC;Targeting IL-17A in multiple myeloma: a potential novel therapeutic approach in myelomaLeukemia26293646med consent in accordance with the Declaration of Helsinki and approved by the institutional review board (IRB) from Dana-Farber Cancer Institute. Healthy donor bone marrow samples were obtained from AllCells (Emeryville, CA). Myeloma cell-proliferaFull Paper
2016Su, LF;Del Alcazar, D;Stelekati, E;Wherry, EJ;Davis, MM;Antigen exposure shapes the ratio between antigen-specific Tregs and conventional T cells in human peripheral bloodProceedings of the National Academy of Sciences of the United States of America27681619Human HLA-DR4 umbilical cord blood was purchased from AllCells and from the New York Human HLA-DR4 umbilical cord bloods were purchased from AllCells and from the New SaveFull Paper
2016Ye, L;Wang, J;Tan, Y;Beyer, AI;Xie, F;Muench, MO;Kan, YW;Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and β-thalassemiaProceedings of the National Academy of Sciences of the United States of America27601644according to the manufacturer’s instruction. Peripheral blood mobilized CD34+ HSPCs and bone marrow CD34+ HSPCs were purchased from AllCells and cultured in StemSpan SFEM (Stemcell Technologies) supplemented with human cytokines, 100 ng/mL SCF, 100 nFull Paper
2016Tarafder, S;Koch, A;Jun, Y;Chou, C;Awadallah, MR;Lee, CH;Micro-precise spatiotemporal delivery system embedded in 3D printing for complex tissue regenerationBiofabrication27108484MSCs were isolated from human bone marrow (AllCells, Alameda, CA) following our established protocol [5, 13]. P3-5 MSCs at a density of 1 × 10 6 cells ml −1 were seeded into the SaveFull Paper
2016Horiguchi, H;Kobune, M;Kikuchi, S;Yoshida, M;Murata, M;Murase, K;Iyama, S;Takada, K;Sato, T;Ono, K;Hashimoto, A;Tatekoshi, A;Kamihara, Y;Kawano, Y;Miyanishi, K;Sawada, N;Kato, J;Extracellular vesicle miR-7977 is involved in hematopoietic dysfunction of mesenchymal stromal cells via poly(rC) binding protein 1 reduction in myeloid neoplasmsHaematologica26802051MSCs from healthy volunteers (HVs) were purchased from AllCells, LLC (Toronto, Canada) (HV-derived MSCs #1, #2, #3 and #4). Human primary MSCs and human BM CD34+ cells derived from lymphoma patients stage I/II without bone marrow localization (controFull Paper
2016Li, D;Xie, K;Zhang, L;Yao, X;Li, H;Xu, Q;Wang, X;Jiang, J;Fang, J;Dual blockade of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) exhibits potent anti-angiogenic effectsCancer letters27130666Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China) and maintained in a complete HUVEC medium with 10% FBS (Invitrogen) and 10% SaveFull Paper
2016de Jong, RN;Beurskens, FJ;Verploegen, S;Strumane, K;van Kampen, MD;Voorhorst, M;Horstman, W;Engelberts, PJ;Oostindie, SC;Wang, G;Heck, AJ;Schuurman, J;Parren, PW;A Novel Platform for the Potentiation of Therapeutic Antibodies Based on Antigen-Dependent Formation of IgG Hexamers at the Cell SurfacePLoS biology26736041nuously cultured systems to ensure authenticity of the cell lines. Pooled normal human serum (NHS) AB was obtained from Sanquin (The Netherlands). Primary B cells from CLL patients were obtained from AllCells (Alameda, CA). Animals Eight- to 11-wk-oFull Paper
2016Carmi, Y;Prestwood, TR;Spitzer, MH;Linde, IL;Chabon, J;Reticker-Flynn, NE;Bhattacharya, N;Zhang, H;Zhang, X;Basto, PA;Burt, BM;Alonso, MN;Engleman, EG;Akt and SHP-1 are DC-intrinsic checkpoints for tumor immunityJCI insight27812544For some experiments, BM cells and peripheral blood from the same donor were purchased from AllCells. The cells were applied to a Ficoll gradient (BD), and CD34 + cells were SaveFull Paper
2016Legemate, K;Tarafder, S;Jun, Y;Lee, CH;Engineering Human TMJ Discs with Protein-Releasing 3D-Printed ScaffoldsJournal of dental research27053116Human MSCs were isolated from commercially available, fresh whole bone marrow samples of anonymous adult donors (AllCells) (age range: 20-25 y) per our previous methods (Lee et al. 2009; Lee, Shah, et al. 2010; Lee et al. 2014). Following our establiFull Paper
2016Kota, DJ;Prabhakara, KS;van Brummen, AJ;Bedi, S;Xue, H;DiCarlo, B;Cox, CS;Olson, SD;Propranolol and Mesenchymal Stromal Cells Combine to Treat Traumatic Brain InjuryStem cells translational medicine26586775We isolated MSCs from commercially available fresh human bone marrow aspirates of a 34-year-old male (AllCells, Emeryville, CA, http://www.allcells.com) according to the protocols previously established using density centrifugation and plastic adhereFull Paper
2016Lee, SJ;Levitsky, K;Parlati, F;Bennett, MK;Arastu-Kapur, S;Kellerman, L;Woo, TF;Wong, AF;Papadopoulos, KP;Niesvizky, R;Badros, AZ;Vij, R;Jagannath, S;Siegel, D;Wang, M;Ahmann, GJ;Kirk, CJ;Clinical activity of carfilzomib correlates with inhibition of multiple proteasome subunits: application of a novel pharmacodynamic assayBritish journal of haematology27071340Human whole blood and peripheral blood mononuclear cell (PBMC)-enriched leukapheresis-derived samples from healthy volunteers were purchased from AllCells (Berkeley, CA, SaveFull Paper
2016Jones, RB;Mueller, S;O'Connor, R;Rimpel, K;Sloan, DD;Karel, D;Wong, HC;Jeng, EK;Thomas, AS;Whitney, JB;Lim, SY;Kovacs, C;Benko, E;Karandish, S;Huang, SH;Buzon, MJ;Lichterfeld, M;Irrinki, A;Murry, JP;Tsai, A;Yu, H;Geleziunas, R;Trocha, A;Ostrowski, MA;Irvine, DJ;Walker, BD;A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-LymphocytesPLoS pathogens27082643the HIV-1 Test, v2.0 kit (Roche Diagnostics). Primary CD4+ T cell HIV latency model Total peripheral blood mononuclear cells (PBMCs) were obtained from healthy HIV-negative donors by leukapheresis (AllCells, Inc, Emeryville, CA). Naive CD4+ T cellsFull Paper
2016Clark, RS;Pellom, ST;Booker, B;Ramesh, A;Zhang, T;Shanker, A;Maguire, M;Juarez, PD;Patricia, MJ;Langston, MA;Lichtveld, MY;Hood, DB;Validation of research trajectory 1 of an Exposome framework: Exposure to benzo(a)pyrene confers enhanced susceptibility to bacterial infectionEnvironmental research26765097Fresh human CD14+ monocytes were obtained from AllCells (Alameda, CA) and were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated FBS (HyClone), 100 U/penicillin, 100 U/mL streptomycin (GIBCO) in the presence of DMSO, B(a)P (1, 5, 10Full Paper
2016Zhang, W;Cao, H;Zhang, X;Li, G;Chang, Q;Zhao, J;Qiao, Y;Ding, X;Yang, G;Liu, X;Jiang, X;A strontium-incorporated nanoporous titanium implant surface for rapid osseointegrationNanoscale26881868In this experiment, endothelial cell growth medium (AllCells) was used to collect the secreted growth factors, and then was taken for HUVECS culture for 24 hours to form cellular SaveFull Paper
2016Wu, J;Sun, Y;Block, TJ;Marinkovic, M;Zhang, ZL;Chen, R;Yin, Y;Song, J;Dean, DD;Lu, Z;Chen, XD;Umbilical cord blood-derived non-hematopoietic stem cells retrieved and expanded on bone marrow-derived extracellular matrix display pluripotent characteristicsStem cell research & therapy27906056Freshly isolated human BM-MNCs (containing MSCs), obtained from 20- to 30-year-old donors and purchased from ALLCELLS (Emeryville, CA, USA), were used to prepare BM stromal SaveFull Paper
2016Umemura, N;Ohkoshi, E;Tajima, M;Kikuchi, H;Katayama, T;Sakagami, H;Hyaluronan induces odontoblastic differentiation of dental pulp stem cells via CD44Stem cell research & therapy27651223s modified Eagle’s medium (DMEM) was purchased from Invitrogen (Carlsbad, CA, USA). Fetal bovine serum (FBS) was purchased from Nichirei Bioscience (Tokyo, Japan). Human DPSCs were obtained from AllCells LLC (Emeryville, CA, USA). Cell cultures wereFull Paper
2016Frasheri, I;Ern, C;Diegritz, C;Hickel, R;Hristov, M;Folwaczny, M;Full-length amelogenin influences the differentiation of human dental pulp stem cellsStem cell research & therapy26762641The human dental pulp stem cells hDPSCs were obtained from AllCells (Alameda, CA, USA). They had been characterized by the company by means of flow cytometry, revealing a SaveFull Paper
2016Chen, W;Wang, S;Adhikari, S;Deng, Z;Wang, L;Chen, L;Ke, M;Yang, P;Tian, R;Simple and Integrated Spintip-Based Technology Applied for Deep Proteome ProfilingAnalytical chemistry27062885The SHED cell line was purchased from Allcells (Alameda, CA) and cultured in α-modified Eagle's medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/ SaveFull Paper
2016Gonçalves Silva, I;Rüegg, L;Gibbs, BF;Bardelli, M;Fruehwirth, A;Varani, L;Berger, SM;Fasler-Kan, E;Sumbayev, VV;The immune receptor Tim-3 acts as a trafficker in a Tim-3/galectin-9 autocrine loop in human myeloid leukemia cellsOncoimmunology27622049). All other chemicals purchased were of the highest grade of purity. Primary human AML and CLL cells Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordaFull Paper
2016Yang, J;Liu, W;Lv, J;Feng, Y;Ren, X;Zhang, W;REDV-polyethyleneimine complexes for selectively enhancing gene delivery in endothelial cellsJournal of materials chemistry. B32263272The pEGFP-ZNF580 plasmid (pZNF580) was preserved by Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force. CREDVW and CRE[V with combining low line][D with combining low line]W peptides were supplieFull Paper
2016Ma, SD;Xu, X;Jones, R;Delecluse, HJ;Zumwalde, NA;Sharma, A;Gumperz, JE;Kenney, SC;PD-1/CTLA-4 Blockade Inhibits Epstein-Barr Virus-Induced Lymphoma Growth in a Cord Blood Humanized-Mouse ModelPLoS pathogens27186886diabetic/severe combined immunodeficient (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson Labs (catalogue 005557). Commercially purchased CD34-depleted human cord blood mononuclear cells (AllCells, LLC., CB117) were mock-infected, or infectFull Paper
2016Ebina, W;Combinatorial Pathway Modulation Toward Ex Vivo Maintenance and Propagation of Hematopoietic Stem CellsThesisCD34 enriched human cord blood, mobilized peripheral blood, or human bone marrow (AllCells) were thawed, washed in PBS with 2mM EDTA and 2%FBS, and stained for the following markers: Lineage(CD235a, CD3, CD19, CD20, CD56), CD34, CD38, CD45RA, and CD9Full Paper
2016Yang, T;Yao, Q;Cao, F;Liu, Q;Liu, B;Wang, XH;Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesisInternational journal of nanomedicine27994464nd the human lymphoma cell lines Raji and Daudi were obtained from the American Type Culture Collection (Manassas, VA, USA). Human umbilical vein endothelial cells (HUVEC) were obtained from Shanghai AllCells Biotech Co. Ltd. (Shanghai, People’s RepuFull Paper
2016Moot, R;Raikar, SS;Fleischer, L;Querrey, M;Tylawsky, DE;Nakahara, H;Doering, CB;Spencer, HT;Genetic engineering of chimeric antigen receptors using lamprey derived variable lymphocyte receptorsMolecular therapy oncolytics27933313d using a BD FACS Aria II Cell Sorter (BD Biosciences). Expansion and lentiviral transduction of primary human T cells Commercially available human peripheral blood mononuclear cells were purchased (AllCells, Catalog # PB004F, Alameda, CA). After thFull Paper
2016Hall, BM;Balan, V;Gleiberman, AS;Strom, E;Krasnov, P;Virtuoso, LP;Rydkina, E;Vujcic, S;Balan, K;Gitlin, I;Leonova, K;Polinsky, A;Chernova, OB;Gudkov, AV;Aging of mice is associated with p16(Ink4a)- and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cellsAging27391570f p16(Ink4a)-positive cells [21,23] and may reflect an exaggerated view on SCs as the sole source of inflammaging. MATERIALS AND METHODS Cell culture Primary human neonatal dermal fibroblasts (NDFs; AllCells, LLC) were pooled equally from three sepaFull Paper
2016Naka, K;Ishihara, K;Jomen, Y;Jin, CH;Kim, DH;Gu, YK;Jeong, ES;Li, S;Krause, DS;Kim, DW;Bae, E;Takihara, Y;Hirao, A;Oshima, H;Oshima, M;Ooshima, A;Sheen, YY;Kim, SJ;Kim, DK;Novel oral transforming growth factor-β signaling inhibitor EW-7197 eradicates CML-initiating cellsCancer science26583567Full Paper
2016Rovira Gonzalez, YI;Lynch, PJ;Thompson, EE;Stultz, BG;Hursh, DA;In vitro cytokine licensing induces persistent permissive chromatin at the Indoleamine 2,3-dioxygenase promoterCytotherapy27421739Bone marrow-derived MSCs were obtained from AllCells (Alameda, CA), Lonza (Allendale, NJ), and RoosterBio (Frederick, MD) at passage one (Supplemental Table 1). MSCs in this study were previously expanded, characterized, and cryopreserved to generateFull Paper
2016Sutton, KS;Dasgupta, A;McCarty, D;Doering, CB;Spencer, HT;Bioengineering and serum free expansion of blood-derived γδ T cellsCytotherapy27260209Commercially available PBMCs were obtained via Leuko Pak apheresis from 11 healthy donors (AllCells). Nine frozen and two fresh PBMCs were used. Frozen PBMCs were rapidly SaveFull Paper
2016Stultz, BG;McGinnis, K;Thompson, EE;Lo Surdo, JL;Bauer, SR;Hursh, DA;Chromosomal stability of mesenchymal stromal cells during in vitro cultureCytotherapy26780865Both AllCells and Lonza use standard protocols for isolation of MSCs from bone marrow and culture cells in Mesencult (Stem Cell Technologies) or MSCGM (Lonza), respectively. SaveFull Paper
2016Imamichi, Y;Yuhki, KI;Orisaka, M;Kitano, T;Mukai, K;Ushikubi, F;Taniguchi, T;Umezawa, A;Miyamoto, K;Yazawa, T;11-Ketotestosterone Is a Major Androgen Produced in Human GonadsThe Journal of clinical endocrinology and metabolism27428878Plasma was separated by centrifugation at 1000g for 5 minutes. Other plasma samples were purchased from AllCells and ProMedDX. All plasma samples were collected under IRB-approved collection protocols and subject informed consent. The donors were 10Full Paper
2016Tsiang, M;Jones, GS;Goldsmith, J;Mulato, A;Hansen, D;Kan, E;Tsai, L;Bam, RA;Stepan, G;Stray, KM;Niedziela-Majka, A;Yant, SR;Yu, H;Kukolj, G;Cihlar, T;Lazerwith, SE;White, KL;Jin, H;Antiviral Activity of Bictegravir (GS-9883), a Novel Potent HIV-1 Integrase Strand Transfer Inhibitor with an Improved Resistance ProfileAntimicrobial agents and chemotherapy27645238the pseudotyped particles from SODk1 2G cells has been previously described (29). Freshly isolated human peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers (supplied by AllCells, Inc., Alameda, CA). Blood donors were neFull Paper
2016Feng, JY;Xu, Y;Barauskas, O;Perry, JK;Ahmadyar, S;Stepan, G;Yu, H;Babusis, D;Park, Y;McCutcheon, K;Perron, M;Schultz, BE;Sakowicz, R;Ray, AS;Role of Mitochondrial RNA Polymerase in the Toxicity of Nucleotide Inhibitors of Hepatitis C VirusAntimicrobial agents and chemotherapy26596942Normal human primary bone marrow (BM) light-density cells were from three different lots obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD). Primary rat neonatal SaveFull Paper
2016Yoon, J;Wang, H;Kim, YC;Yoshimoto, M;Abbasi, S;Morse Iii, HC;Plasma cell alloantigen ENPP1 is expressed by a subset of human B cells with potential regulatory functionsImmunology and cell biology270298961 mm sodium pyruvate and HEPES) immediately before each experiment. Cord blood cells were purchased from AllCells LLC (Emeryville, CA. USA). Tonsillar tissues were kindly provided by Dr Leonid B Margolis (National Institute of Child Health and HumanFull Paper
2016Marklein, RA;Lo Surdo, JL;Bellayr, IH;Godil, SA;Puri, RK;Bauer, SR;High Content Imaging of Early Morphological Signatures Predicts Long Term Mineralization Capacity of Human Mesenchymal Stem Cells upon Osteogenic InductionStem cells (Dayton, Ohio)26865267Human bone marrow-derived stem cells were obtained from eight different donors purchased from either Lonza (Walkersville, MD, www.lonza.com) (167696, 110877, 8F3560, 127756) or All Cells (Emeryville, CA, www.allcells.com) (PCBM1632, PCBM1641, PCBM165Full Paper
2016Rosa, V;Xie, H;Dubey, N;Madanagopal, TT;Rajan, SS;Morin, JL;Islam, I;Castro Neto, AH;Graphene oxide-based substrate: physical and surface characterization, cytocompatibility and differentiation potential of dental pulp stem cellsDental materials : official publication of the Academy of Dental Materials27283997The use of human cells in this study was approved by NUS Institutional Review Board (Approval Number: NUS 2094). Human dental pulp stem cells from single donor (DPF003, Allcells, USA) were sorted by fluorescence-activated cell sorting analysis (FACS,Full Paper
2016Wang, J;Zhang, Y;Zhu, Q;Liu, Y;Cheng, H;Zhang, Y;Li, T;Emodin protects mice against radiation-induced mortality and intestinal injury via inhibition of apoptosis and modulation of p53Environmental toxicology and pharmacology27525562The aim of this study was to explore the protective effect of emodin, a plant-derived anthraquinone, against gamma radiation-induced mortality and intestinal injury in mice, and to SaveFull Paper
2016Walters, MC;De Castro, LM;Sullivan, KM;Krishnamurti, L;Kamani, N;Bredeson, C;Neuberg, D;Hassell, KL;Farnia, S;Campbell, A;Petersdorf, E;Indications and Results of HLA-Identical Sibling Hematopoietic Cell Transplantation for Sickle Cell DiseaseBiology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation26500093Financial disclosure: MCW is medical director for the ViaCord Processing Laboratory and AllCells, Inc. He is a consultant for bluebird bio, Inc, Sangamo Biosciences, Inc and Bayer. SaveFull Paper
2016Hsu, H;Boudova, S;Mvula, G;Divala, TH;Mungwira, RG;Harman, C;Laufer, MK;Pauza, CD;Cairo, C;Prolonged PD1 Expression on Neonatal Vδ2 Lymphocytes Dampens Proinflammatory Responses: Role of Epigenetic RegulationJournal of immunology (Baltimore, Md. : 1950)27474072commercial specimens obtained from AllCells. Although we cannot exclude that we introduced a bias in our study, this seems unlikely considering that the AllCells specimens were not SaveFull Paper
2016Chen, LT;Hu, MM;Xu, ZS;Liu, Y;Shu, HB;MSX1 Modulates RLR-Mediated Innate Antiviral Signaling by Facilitating Assembly of TBK1-Associated ComplexesJournal of immunology (Baltimore, Md. : 1950)27194789Generation and transfection of human primary macrophages were performed, as previously described (36). The human CD14+ monocytes were purchased from ALLCELLS. For generation of macrophages, the CD14+ monocytes were treated with recombinant M-CSF (50Full Paper
2016Gao, W;Liu, H;Yuan, J;Wu, C;Huang, D;Ma, Y;Zhu, J;Ma, L;Guo, J;Shi, H;Zou, Y;Ge, J;Exosomes derived from mature dendritic cells increase endothelial inflammation and atherosclerosis via membrane TNF-α mediated NF-κB pathwayJournal of cellular and molecular medicine27515767P (Ribobio, Guangzhou, China) was used. SiRNA TNF‐α was also purchased from Ribobio and the transfected concentration was 50 nM. HUVECs culture and intervention Primary HUVECs were purchased from AllCells (Shanghai, China) and cultured with a speciaFull Paper
2016Zhou, Y;Li, XH;Zhang, CC;Wang, MJ;Xue, WL;Wu, DD;Ma, FF;Li, WW;Tao, BB;Zhu, YC;Hydrogen sulfide promotes angiogenesis by downregulating miR-640 via the VEGFR2/mTOR pathwayAmerican journal of physiology. Cell physiology26879375Pooled human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in endothelial basal medium (EBM; Allcells) supplemented with SaveFull Paper
2016Lowery, JW;Ankrum, JA;Kokabu, S;Liu, R;Targeted Strategies to Modulate Stem-Cell-Relevant PathwaysStem cells international27703484+ CB progenitors (also referred to as "starting CB progenitors") from pooled donors were purchased from AllCells (Emeryville, CA) and cultured in the hematopoietic growth medium ( SaveFull Paper
2016Nagaria, PK;Robert, C;Park, TS;Huo, JS;Zambidis, ET;Rassool, FV;High-Fidelity Reprogrammed Human IPSCs Have a High Efficacy of DNA Repair and Resemble hESCs in Their MYC Transcriptional SignatureStem cells international27688775+ CB progenitors (also referred to as "starting CB progenitors") from pooled donors were purchased from AllCells (Emeryville, CA) and cultured in the hematopoietic growth medium ( SaveFull Paper
2016Shibata, T;Ohto, U;Nomura, S;Kibata, K;Motoi, Y;Zhang, Y;Murakami, Y;Fukui, R;Ishimoto, T;Sano, S;Ito, T;Shimizu, T;Miyake, K;Guanosine and its modified derivatives are endogenous ligands for TLR7International immunology26489884Human macrophages were induced from human peripheral blood mononuclear cells (hPBMCs), purchased from Allcells (Alameda, CA, USA) and Cellular Technology Limited (Shaker Height, OH, USA). hPBMCs were plated in 10-cm cell-culture dishes with a densityFull Paper
2016Pineda, G;Lennon, KM;Delos Santos, NP;Lambert-Fliszar, F;Riso, GL;Lazzari, E;Marra, MA;Morris, S;Sakaue-Sawano, A;Miyawaki, A;Jamieson, CH;Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined NicheScientific reports27041210amples were obtained from patient peripheral blood that was collected with informed consent for research use in accordance with Institutional Review Board approved protocols at UCSD or purchased from AllCells (Alameda, CA). Normal peripheral blood moFull Paper
2016Zou, L;Chen, Q;Quanbeck, Z;Bechtold, JE;Kaufman, DS;Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cellsScientific reports26980556Human BM-MSCs were isolated from fresh bone marrow (AllCells) according to the recommended protocol and expanded in MSC culture media, consisting of 10% defined fetal bovine SaveFull Paper
2016Ling, C;Bhukhai, K;Yin, Z;Tan, M;Yoder, MC;Leboulch, P;Payen, E;Srivastava, A;High-Efficiency Transduction of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for Overcoming Donor-Variation and Implications in Genome EditingScientific reports27759036ary HSPCs derived from bone marrow (BM) as well as from umbilical cord blood (CB). BM-derived CD34+ cells from individual donors (or a mixture from 10 donors) were purchased from a commercial source (AllCells, LLC, Alameda, CA, USA), and were used toFull Paper
2016Tian, GA;Zhu, CC;Zhang, XX;Zhu, L;Yang, XM;Jiang, SH;Li, RK;Tu, L;Wang, Y;Zhuang, C;He, P;Li, Q;Cao, XY;Cao, H;Zhang, ZG;CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinibScientific reports27506146HUVECs were obtained from the Fifth People's Hospital of Shanghai and cultured in endothelial cell complete medium containing endothelial cell growth supplement (Allcells; H-004). SaveFull Paper
2016Kim, YC;Cui, J;Luo, J;Xiao, F;Downs, B;Wang, SM;Exome-based Variant Detection in Core PromotersScientific reports27464681Caucasian male donor were purchased from AllCells Company (AllCells, Alameda, California). The use of the cells for research purpose has AllCells company IRB approval (Biomed SaveFull Paper
2016Chalmers, SA;Doerner, J;Bosanac, T;Khalil, S;Smith, D;Harcken, C;Dimock, J;Der, E;Herlitz, L;Webb, D;Seccareccia, E;Feng, D;Fine, JS;Ramanujam, M;Klein, E;Putterman, C;Therapeutic Blockade of Immune Complex-Mediated Glomerulonephritis by Highly Selective Inhibition of Bruton's Tyrosine KinaseScientific reports27192942Primary CD19+ cells were purified from human peripheral blood mononuclear cells (AllCells frozen peripheral blood mononuclear cells (AllCells) and negatively selected by magnetic SaveFull Paper
2016Moisseiev, E;Smit-McBride, Z;Oltjen, S;Zhang, P;Zawadzki, RJ;Motta, M;Murphy, CJ;Cary, W;Annett, G;Nolta, JA;Park, SS;Intravitreal Administration of Human Bone Marrow CD34+ Stem Cells in a Murine Model of Retinal DegenerationInvestigative ophthalmology & visual science27537262Fresh human BM aspirate was purchased from AllCells (Alameda, CA, USA) and obtained for the scheduled day of use. The BM aspirate for this study was obtained from a 38-year-old SaveFull Paper
2016Hassan, HE;Keita, JA;Narayan, L;Brady, SM;Frederick, R;Carlson, S;C Glass, K;Natesan, S;Buttolph, T;Fandy, TE;The combination of dimethoxycurcumin with DNA methylation inhibitor enhances gene re-expression of promoter-methylated genes and antagonizes their cytotoxic effectEpigenetics27588609Frozen acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) bone marrow mononuclear cells (BMNC) were purchased from Allcells (Alameda, CA). BMNC were obtained from newly diagnosed and untreated patients. The viability of the cells was tesFull Paper
2016Hu, X;Hu, T;Shen, G;Lian, M;Guan, G;Wang, F;Wang, L;PCL films of varying porosity influence ICAM-1 expression of HUVECsJournal of biomedical materials research. Part A27345288The HUVECs (HUVEC001) provided by Allcells Company (Shanghai, China) were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco), and 1% penicillin-streptomycin (Gibco) at 37°C in a 5% CO2 incubator. Before seeFull Paper
2016Lin, HA;Gupta, MS;Varma, DM;Gilchrist, ML;Nicoll, SB;Lower crosslinking density enhances functional nucleus pulposus-like matrix elaboration by human mesenchymal stem cells in carboxymethylcellulose hydrogelsJournal of biomedical materials research. Part A26256108Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density gradient centrifugation as previously described.18 Briefly, bone marrow aspirate was SaveFull Paper
2016Rogers, LJ;Basnakian, AG;Orloff, MS;Ning, B;Yao-Borengasser, A;Raj, V;Kadlubar, S;2-amino-1-methyl-6-phenylimidazo(4,5-b) pyridine (PhIP) induces gene expression changes in JAK/STAT and MAPK pathways related to inflammation, diabetes and cancerNutrition & metabolism27547236med by submission to American Type Culture Collection (ATCC) (Manassas, VA) for analysis by short tandem repeat methodology on December 9, 2015. Human Mesenchymal Stem Cells (hMSC) were obtained from AllCells (Emeryville, CA). The hMSCs were expandedFull Paper
2016Pellegrino, M;Sciambi, A;Yates, JL;Mast, JD;Silver, C;Eastburn, DJ;RNA-Seq following PCR-based sorting reveals rare cell transcriptional signaturesBMC genomics27189161Anonymous blood samples were initially obtained from a commercial provider (AllCells) that operates with independent IRB approval. Donor anonymity was protected using United SaveFull Paper
2016Holt, A;Wootton, SC;Mulero, JJ;Brzoska, PM;Langit, E;Green, RL;Developmental validation of the Quantifiler(®) HP and Trio Kits for human DNA quantification in forensic samplesForensic science international. Genetics26774100Human female DNA "A3408" was purified from peripheral blood cells obtained from AllCells LLC. (Alameda, CA) using a QIAamp DNA Blood Maxi Kit (Qiagen). SaveFull Paper
2016Pankajakshan, D;Albuquerque, MT;Evans, JD;Kamocka, MM;Gregory, RL;Bottino, MC;Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell FunctionJournal of endodontics27663615After 7 days, the specimens were rinsed with 10 mL saline followed by 3 mL EDTA for 3 minutes, and a final abundant rinsing step with saline was performed before cell seeding. Human DPSCs (AllCells, LLC, Alameda, CA) were cultured in alpha-minimum esFull Paper
2016Nicoletti, AM;Kenny, CH;Khalil, AM;Pan, Q;Ralph, KL;Ritchie, J;Venkataramani, S;Presky, DH;DeWire, SM;Brodeur, SR;Unexpected Potency Differences between B-Cell-Activating Factor (BAFF) Antagonist Antibodies against Various Forms of BAFF: Trimer, 60-Mer, and Membrane-BoundThe Journal of pharmacology and experimental therapeutics27440419Negatively selected B cells were obtained from frozen human peripheral blood (AllCells, LLC, Alameda, CA). Sexes of cell donors were as follows: Fig. 2C = 60-mer, two males, one SaveFull Paper
2016Jin, E;Kim, TH;Han, S;Kim, SW;Amniotic epithelial cells promote wound healing in mice through high epithelialization and engraftmentJournal of tissue engineering and regenerative medicine26174407Human AMEs were from AllCells (Alameda, CA, USA) and adipose-derived mesenchymal stem cells (ADMs) were from Thermo Scientific Inc. (Rockford, IL, USA). Human keratinocytes were also purchased from ATCC. Cell culture was performed as described previoFull Paper
2016Yang, H;Sun, J;Li, Y;Duan, WM;Bi, J;Qu, T;Human umbilical cord-derived mesenchymal stem cells suppress proliferation of PHA-activated lymphocytes in vitro by inducing CD4(+)CD25(high)CD45RA(+) regulatory T cell production and modulating cytokine secretionCellular immunology26774852PBMCs of healthy donors (n = 10) were purchased from AllCells Inc (Emeryville, CA, USA) and StemExpress (Placerville, CA, USA). Co-culture experiments were performed in a 12-well plate (BD, Franklin Lakes, New Jersey, USA). 1 × 105 UC-MSCs were plateFull Paper
2016Foster, MH;Buckley, ES;Chen, BJ;Hwang, KK;Clark, AG;Uncommon structural motifs dominate the antigen binding site in human autoantibodies reactive with basement membrane collagenMolecular immunology27450516100 cGy, X-RAD 320, Precision X-ray, North Branford, CT) 9.5-10 week old NSG recipients were intravenously injected with purified human CD34+ HSC (AllCells LLC, Alameda, CA), using 2.5-10×104 CD34+ HSC per mouse (Pearson et al., 2008; Shultz et al.,Full Paper
2016Smith, AJ;Li, Y;Bazin, HG;St-Jean, JR;Larocque, D;Evans, JT;Baldridge, JR;Evaluation of novel synthetic TLR7/8 agonists as vaccine adjuvantsVaccine27402566Human myeloid or plasmacytoid DCs were isolated from PBMCs obtained by leukapheresis from normal donors (AllCells, Berkeley, CA). PBMCs were repeatedly washed in 1× PBS SaveFull Paper
2016Zeng, Y;Liu, J;Role of glypican-1 in endothelial NOS activation under various steady shear stress magnitudesExperimental cell research27688027Human umbilical vein endothelial cells (HUVECs) and all cell culture reagents were purchased from Allcells, China. As previously described [23], HUVECs were cultured in HUVEC medium containing 10% FBS and 10% growth factor supplement containing EGF,Full Paper
2016Liu, X;Zhang, A;Xiang, J;Lv, Y;Zhang, X;miR-451 acts as a suppressor of angiogenesis in hepatocellular carcinoma by targeting the IL-6R-STAT3 pathwayOncology reports27461244The umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Shanghai, China). HepG2 and HEK293T cells were incubated with Dulbecco's modified Eagle's medium ( SaveFull Paper
2016Peterson, SJ;Vanella, L;Gotlinger, K;Jiang, H;Singh, SP;Sodhi, K;Maher, E;O'Hanlon, K;Shapiro, JI;Abraham, NG;Oxidized HDL is a potent inducer of adipogenesis and causes activation of the Ang-II and 20-HETE systems in human obese femalesProstaglandins & other lipid mediators27179555Frozen human bone marrow mononuclear stem cells (MSCs) were purchased from Allcells (Allcells, Emeryville, CA). After thawing, mononuclear cells were resuspended in a minimal essential medium, (Invitrogen, Carlsbad, CA) supplemented with 10% heat inaFull Paper
2016Ren, X;Li, J;Xu, X;Wang, C;Cheng, Y;IBI302, a promising candidate for AMD treatment, targeting both the VEGF and complement system with high binding affinity in vitro and effective targeting of the ocular tissue in healthy rhesus monkeysExperimental eye research26919788HUVEC basal medium (used for HUVEC proliferation assay) was bought from Allcells (Shanghai, China). Rabbit red blood cells (RRBCs) were purchased from Laboratory animal SaveFull Paper
2016Griffin, DO;Goff, SP;Restriction of HIV-1-based lentiviral vectors in adult primary marrow-derived and peripheral mobilized human CD34+ hematopoietic stem and progenitor cells occurs prior to viral DNA integrationRetrovirology26945863ep toward uncovering the molecular mechanism restricting HIV-1 transduction of HPSCs. Methods Source of human cells We obtained primary adult marrow-derived and peripheral mobilized CD34+ cells from AllCells and StemCell Technologies (pre-isolated).Full Paper
2016Cao, L;Weetall, M;Bombard, J;Qi, H;Arasu, T;Lennox, W;Hedrick, J;Sheedy, J;Risher, N;Brooks, PC;Trifillis, P;Trotta, C;Moon, YC;Babiak, J;Almstead, NG;Colacino, JM;Davis, TW;Peltz, SW;Discovery of Novel Small Molecule Inhibitors of VEGF Expression in Tumor Cells Using a Cell-Based High Throughput Screening PlatformPloS one27992500All primary cells were purchased from AllCells, Inc (California, USA) and cultured in RPMI-1640 medium for peripheral blood mononuclear cells (PBMC) or DMEM for adherent cells, SaveFull Paper
2016Pogue, SL;Taura, T;Bi, M;Yun, Y;Sho, A;Mikesell, G;Behrens, C;Sokolovsky, M;Hallak, H;Rosenstock, M;Sanchez, E;Chen, H;Berenson, J;Doyle, A;Nock, S;Wilson, DS;Targeting Attenuated Interferon-α to Myeloma Cells with a CD38 Antibody Induces Potent Tumor Regression with Reduced Off-Target ActivityPloS one27611189Bone Marrow cells: Normal and myeloma bone marrow cells were purchased from AllCells, Inc. as frozen vials containing ten million bone marrow mononuclear cells/vial. All donors SaveFull Paper
2016Doss, JF;Jonassaint, JC;Garrett, ME;Ashley-Koch, AE;Telen, MJ;Chi, JT;Phase 1 Study of a Sulforaphane-Containing Broccoli Sprout Homogenate for Sickle Cell DiseasePloS one27071063preferably the same time of day, once daily for 21 days. Patients consumed one (50g), two (100g) or three (150g) packages, depending on the dose. Cell culture Mobilized peripheral blood CD34+ cells (AllCells) were grown in StemLine II media containiFull Paper
2016Maifeld, SV;Ro, B;Mok, H;Chu, M;Yu, L;Yamagata, R;Leonardson, T;Chio, V;Parhy, B;Park, S;Carlson, M;Machhi, S;Ulbrandt, N;Falsey, AR;Walsh, EE;Wang, CK;Esser, MT;Zuo, F;Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial VirusPloS one27070145serum samples showing a ≥4-fold rise in at least one ELISA assay. Thirty pediatric serum samples were purchased from Bioreclamation (Hicksville, NY). An adult human plasma sample was purchased from AllCells (Emeryville, CA) and subsequently used as tFull Paper
2016Smith, SH;Peredo, CE;Takeda, Y;Bui, T;Neil, J;Rickard, D;Millerman, E;Therrien, JP;Nicodeme, E;Brusq, JM;Birault, V;Viviani, F;Hofland, H;Jetten, AM;Cote-Sierra, J;Development of a Topical Treatment for Psoriasis Targeting RORγ: From Bench to SkinPloS one26870941ions were carried out in triplicate. Sequences of primers for ChIP-qPCR are listed in S1 Appendix. Human peripheral blood CD4+ T cell cultures and cytokine analysis Cryopreserved human CD4+ T cells (AllCells, LLC and Stemcell Technologies, Inc.) werFull Paper
2016Toby, GG;Liu, T;Buyue, Y;Zhang, X;Bitonti, AJ;Pierce, GF;Sommer, JM;Jiang, H;Peters, RT;Recombinant Factor IX Fc Fusion Protein Maintains Full Procoagulant Properties and Exhibits Prolonged Efficacy in Hemophilia B MicePloS one26840952e preparation (L-α-phosphatidylserine [PS] and L-α-phosphatidylcholine [PC]) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Fresh platelets from normal volunteers were purchased from AllCells, LLC (Emeryville, CA, USA). The mini-extrudFull Paper
2016Rebbapragada, I;Birkus, G;Perry, J;Xing, W;Kwon, H;Pflanz, S;Molecular Determinants of GS-9620-Dependent TLR7 ActivationPloS one26784926Human PBMCs were isolated from fresh whole blood derived from healthy human volunteers (AllCells) using standard Ficoll density gradient separation techniques. PBMCs were SaveFull Paper
2016Matsushita, M;Otsuka, Y;Tsutsumida, N;Tanaka, C;Uchiumi, A;Ozawa, K;Suzuki, T;Ichikawa, D;Aburatani, H;Okamoto, S;Kawakami, Y;Hattori, Y;Identification of Novel HLA-A*24:02-Restricted Epitope Derived from a Homeobox Protein Expressed in Hematological MalignanciesPloS one26784514D, Oslo, Norway) and cryopreserved in Cell Banker reagent (Juji-Field, Tokyo, Japan) in liquid nitrogen until further use. Bone marrow mononuclear cells (BMMNC) from healthy donors were obtained from AllCells, LLC. (Alameda, CA). This study was approFull Paper
2016Manser, M;Influence of extremely-low-frequency magnetic fields on epigenetic programming and cellular differentiationThesistrichostatin A (TSA). CD34-positive cells isolated from human cord blood of mixed donors were obtained from AllCells (Alabama, United States). CD34+ cells were propagated in Stemline II medium expansion medium (Stemcell technology) supplemented withFull Paper
2016Cabrero, M;Wei, Y;Yang, H;Ganan-Gomez, I;Bohannan, Z;Colla, S;Marchesini, M;Bravo, GM;Takahashi, K;Bueso-Ramos, C;Garcia-Manero, G;Down-regulation of EZH2 expression in myelodysplastic syndromesLeukemia research26970171Bone marrows from healthy individuals were obtained from AllCells (Emeryville, CA). Bone marrow mononuclear cells were isolated using a standard Ficoll gradient approach. Isolation SaveFull Paper
2016Salzano, G;Zappavigna, S;Luce, A;D'Onofrio, N;Balestrieri, ML;Grimaldi, A;Lusa, S;Ingrosso, D;Artuso, S;Porru, M;Leonetti, C;Caraglia, M;De Rosa, G;Transferrin-Targeted Nanoparticles Containing Zoledronic Acid as a Potential Tool to Inhibit Glioblastoma GrowthJournal of biomedical nanotechnology27301207Viable human bone marrow cells (purchased as frozen vials from AllCells via StemCell Technologies) were prepared with a DNase treatment (Stem Cell Technologies) and then SaveFull Paper
2016Phillips, JE;Renteria, L;Burns, L;Harris, P;Peng, R;Bauer, CM;Laine, D;Stevenson, CS;Btk Inhibitor RN983 Delivered by Dry Powder Nose-only Aerosol Inhalation Inhibits Bronchoconstriction and Pulmonary Inflammation in the Ovalbumin Allergic Mouse Model of AsthmaJournal of aerosol medicine and pulmonary drug delivery27111445Mast cells One million human cord blood derived CD34+ hematopoietic stem cells (HSCs) from different donors (AllCells #CB008F-S, Emeryville, CA) were cultured for 8 weeks in a serum-free complete medium (StemPro-34 with supplements; Invitrogen, CarlsFull Paper
2016Wang, X;Zhao, S;Su, M;Sun, L;Zhang, S;Wang, D;Liu, Z;Yuan, Y;Liu, Y;Li, Y;Geraniol improves endothelial function by inhibiting NOX-2 derived oxidative stress in high fat diet fed miceBiochemical and biophysical research communications27107694In the present study, the in vivo diet induced obese setting was reproduced in vitro by exposing HUVECs to palmitic acid (PA), the most common circulating free fatty acid, with or without geraniol. Human umbilical vein endothelial cells (HUVECs) wereFull Paper
2016Xia, T;Guan, W;Fu, J;Zou, X;Han, Y;Chen, C;Zhou, L;Zeng, C;Wang, WE;Tirofiban induces vasorelaxation of the coronary artery via an endothelium-dependent NO-cGMP signaling by activating the PI3K/Akt/eNOS pathwayBiochemical and biophysical research communications27018249Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCELLS (Shanghai, China). HUVECs were cultured at 37 °C on gelatin-coated plates in the basal nutrient media MCDB-131 supplemented with 5% fetal bovine serum (FBS; Hyclone, Logan,Full Paper
2016Cao, Z;Wang, H;Mao, X;Luo, L;Noncanonical function of threonyl-tRNA synthetase regulates vascular development in zebrafishBiochemical and biophysical research communications26993167Human umbilical vein endothelial cells (HUVECs, ALLCELLS) were raised according to standard protocol. Cell immunostaining was performed as previously described [15] using SaveFull Paper
2016Sugino, N;Miura, Y;Yao, H;Iwasa, M;Fujishiro, A;Fujii, S;Hirai, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T;Early osteoinductive human bone marrow mesenchymal stromal/stem cells support an enhanced hematopoietic cell expansion with altered chemotaxis- and adhesion-related gene expression profilesBiochemical and biophysical research communications26707642Normal human BM samples that were obtained from healthy adult volunteers with informed consent were purchased from AllCells (Emeryville, CA). Human BM-MSCs were isolated and cultured based on a previously published method [3], [12], [13], [14], [15].Full Paper
2016Koestler, DC;Jones, MJ;Usset, J;Christensen, BC;Butler, RA;Kobor, MS;Wiencke, JK;Kelsey, KT;Improving cell mixture deconvolution by identifying optimal DNA methylation libraries (IDOL)BMC bioinformatics26956433Purified granulocytes, monocytes, CD4T, CD8T, natural killer cells, and B cells from normal human subjects were purchased from AllCells LLC (Emeryville, CA). As described (http://www SaveFull Paper
2016Sarkar, S;Sabhachandani, P;Stroopinsky, D;Palmer, K;Cohen, N;Rosenblatt, J;Avigan, D;Konry, T;Dynamic analysis of immune and cancer cell interactions at single cell level in microfluidic dropletsBiomicrofluidics27795747RPMI-8226 (multiple myeloma (MM) cell line) was purchased from the American Type Culture Collection (ATCC, Manassas, VA). CD8+ T cells were purchased from AllCells (#PB009-3F, AllCells, Alameda, CA). Cells were maintained in RPMI-1640 medium supplemeFull Paper
2016Kikushige, Y;Akashi, K;A TIM-3/GAL-9 autocrine stimulatory loop drives self-renewal of human myeloid leukemia stem cells and leukemic progressionExperimental HematologyHuman adult BM and PB samples were obtained from healthy donors or purchased from AllCells. CB cells were obtained from full-term deliveries (provided by the Kyushu Block Red SaveFull Paper
2016Itokazu, M;Wakitani, S;Mera, H;Tamamura, Y;Sato, Y;Takagi, M;Nakamura, H;Transplantation of Scaffold-Free Cartilage-Like Cell-Sheets Made from Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair: A Preclinical StudyCartilage27688844hBMSCs were prepared from three male donors aged 21 (#1), 27 (#2), and 31 (#3) years and from one commercially available bone marrow (Allcells LLC, Alameda, CA, USA) from a 20-year-old male (#4). The donor cells were derived from bone marrow aspirateFull Paper
2016Yan, Z;Liu, J;Xie, L;Liu, X;Zeng, Y;Role of heparan sulfate in mediating CXCL8-induced endothelial cell migrationPeerJ26870616Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells, China. HUVECs were grown in complete culture media for HUVECs (Allcells, China) in a humidified 5%/ SaveFull Paper
2016Hara, C;Kubota, S;Nishida, T;Hiasa, M;Hattori, T;Aoyama, E;Moriyama, Y;Kamioka, H;Takigawa, M;Involvement of multiple CCN family members in platelets that support regeneration of joint tissuesModern rheumatology26915735Human platelets from a healthy donor were provided by AllCells LLC (Berkley, CA). Protein extraction from the cells was performed with RIPA buffer (50 mM Tris-HCl, 0.15 M NaCl, 4 SaveFull Paper
2016Zhang, M;Jiang, L;Oxidized low-density lipoprotein decreases VEGFR2 expression in HUVECs and impairs angiogenesisExperimental and therapeutic medicine28105106Cell culture and LDL treatment In total 2 × 105 HUVECs (AllCells, LLC, Shanghai, China) were cultured for 3-5 passages in Dulbecco's modified Eagle's medium (DMEM; PromoCell GmbH, Heidelberg, Germany) supplemented with 10% fetal bovine serum (unlessFull Paper
2016Heng, BC;Zhu, S;Xu, J;Yuan, C;Gong, T;Zhang, C;Effects of decellularized matrices derived from periodontal ligament stem cells and SHED on the adhesion, proliferation and osteogenic differentiation of human dental pulp stem cells in vitroTissue & cell26796232Human DPSC (Cat No. DP003F) and SHED (Cat No. DP004F) were obtained from AllCells LLC. (Alameda, CA, USA). Human PDLSC were obtained as a gift from the School of Stomatology of Fujian Medical School (Fujian, China) and were isolated as previously desFull Paper
2016Klingelhöffer, C;Reck, A;Morsczeck, C;The extracellular concentration of osteocalcin decreased in dental follicle cell cultures during biomineralizationCytotechnology27447177Human dental follicle cells (DFCs) were purchased from ALLCells (Alameda, CA, USA). DFCs were cultivated in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, Munich, Germany), supplemented with 10 % fetal bovine serum, FBS (Sigma-Aldrich) and 1Full Paper
2016Ahmed, HM;Luddin, N;Kannan, TP;Mokhtar, KI;Ahmad, A;Chemical analysis and biological properties of two different formulations of white portland cementsScanning26382064DPSCs cell line (AllCells, USA) obtained from biopsies of the dental pulp of adult teeth was (BCGM) (AllCells, USA), with 50 mL of human MSC stimulatory supplements (AllCells, USA). SaveFull Paper
2016Mulato, A;Hansen, D;Thielen, A;Porter, D;Stepan, G;White, K;Daeumer, M;Cihlar, T;Yant, SR;Rapid In Vitro Evaluation of Antiretroviral Barrier to Resistance at Therapeutic Drug LevelsAIDS research and human retroviruses27356854Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh leukopaks obtained from healthy volunteers under informed consent (AllCells, Inc., Alameda, CA) and SaveFull Paper
2016Kowolik, CM;Lin, M;Xie, J;Overman, LE;Horne, DA;NT1721, a novel epidithiodiketopiperazine, exhibits potent in vitro and in vivo efficacy against acute myeloid leukemiaOncotarget27863389We then tested the effect of NT1721 on primary AML samples (from AllCells, obtained from two newly diagnosed AML patients with white blood cell counts > 10 11 /L) by treating the SaveFull Paper
2016Jochems, C;Hodge, JW;Fantini, M;Fujii, R;Morillon, YM;Greiner, JW;Padget, MR;Tritsch, SR;Tsang, KY;Campbell, KS;Klingemann, H;Boissel, L;Rabizadeh, S;Soon-Shiong, P;Schlom, J;An NK cell line (haNK) expressing high levels of granzyme and engineered to express the high affinity CD16 alleleOncotarget27861156um iodide (AO/PI) staining on a Cellometer Auto 2000 (Nexcelom, San Mateo, CA), according to the manufacturer's instructions. Release of IL-2 into culture supernatants was measured with an ELISA kit (AllCells, Alameda, CA). Antibodies and flow cytomFull Paper
2016Gołąb, K;Grose, R;Trzonkowski, P;Wickrema, A;Tibudan, M;Marek-Trzonkowska, N;Matosz, S;Solomina, J;Ostrega, D;Michael Millis, J;Witkowski, P;Utilization of leukapheresis and CD4 positive selection in Treg isolation and the ex-vivo expansion for a clinical application in transplantation and autoimmune disordersOncotarget27821811We obtained de-identified human cell leukapheresis product for the all experiments from the commercial vendor AllCells, Alameda, CA, USA, therefore the study was exempt from the SaveFull Paper
2016Yang, H;Sun, J;Wang, F;Li, Y;Bi, J;Qu, T;Umbilical cord-derived mesenchymal stem cells reversed the suppressive deficiency of T regulatory cells from peripheral blood of patients with multiple sclerosis in a co-culture - a preliminary studyOncotarget27705922RRMS, n=12, female=8, male=4), mean age 53.75 years old (49 years to 64 years old) and healthy donors (n=10, female=4, male=6), mean age 28.38 years old (22 years to 39 years old) were purchased from AllCells Inc (Emeryville, CA) or StemExpress (PlacFull Paper
2016Xu, Y;Wang, K;Yu, Q;FRMD6 inhibits human glioblastoma growth and progression by negatively regulating activity of receptor tyrosine kinasesOncotarget27661120entific. Secondary anti-rabbit Alexa Fluor 594 and anti-mouse Alexa Fluor 488 were from Invitrogen. Cells, GBM cell lines, and primary GBM cells Normal human astrocytes (NHAs) were obtained from ALLCELLS, Inc and ScienCell. SF126, SF188, SF210, SFFull Paper
2016Ferluga, S;Tomé, CM;Herpai, DM;D'Agostino, R;Debinski, W;Simultaneous targeting of Eph receptors in glioblastomaOncotarget27494882r Center of Wake Forest University). Stacked 2D images were obtained in some cases. Images were processed with OlyVIA V2.8. Dendritic cells Myeloid-derived naïve dendritic cells were purchased from AllCells LLC (Alameda, CA). Cells were grown in RPMFull Paper
2016Sumbayev, VV;Gonçalves Silva, I;Blackburn, J;Gibbs, BF;Yasinska, IM;Garrett, MD;Tonevitsky, AG;Ushkaryov, YA;Expression of functional neuronal receptor latrophilin 1 in human acute myeloid leukaemia cellsOncotarget27322212oetal calf serum, penicillin (50 IU/ml) and streptomycin sulphate (50 μg/ml). Primary human AML cells Primary human AML mononuclear cells (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordanFull Paper
2016Wang, Y;Zhan, Z;Jiang, X;Peng, X;Shen, Y;Chen, F;Ji, Y;Liu, W;Shi, Y;Duan, W;Ding, J;Ai, J;Geng, M;Simm530, a novel and highly selective c-Met inhibitor, blocks c-Met-stimulated signaling and neoplastic activitiesOncotarget27191264rom Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine (Shanghai, China). SK-OV-3 was obtained from Fudan University (Shanghai, China). HUVEC was obtained from AllCells Bio (Shanghai, China). Cells were routineFull Paper
2016Ye, BG;Sun, HC;Zhu, XD;Chai, ZT;Zhang, YY;Ao, JY;Cai, H;Ma, DN;Wang, CH;Qin, CD;Gao, DM;Tang, ZY;Reduced expression of CD109 in tumor-associated endothelial cells promotes tumor progression by paracrine interleukin-8 in hepatocellular carcinomaOncotarget27121053(Sigma-Aldrich, St. Louis, MO, USA) in a humidified atmosphere of 5% CO2 at 37°C. Human normal liver L-02 cells (Chinese Academy of Sciences) were cultured under the same conditions. Primary HUVEC (AllCells, Shanghai, China) were cultured in endothelFull Paper
2016Schwaederle, M;Husain, H;Fanta, PT;Piccioni, DE;Kesari, S;Schwab, RB;Banks, KC;Lanman, RB;Talasaz, A;Parker, BA;Kurzrock, R;Detection rate of actionable mutations in diverse cancers using a biopsy-free (blood) circulating tumor cell DNA assayOncotarget26848768yzed for 54 genes via NGS in circulating tumor, cell-free DNA. RESULTS Analysis of control samples from healthy persons During the technology development process, 79 healthy normal controls (source: AllCells, http://www.allcells.com) were tested andFull Paper
2016Li, Y;Wang, Y;Li, L;Kong, R;Pan, S;Ji, L;Liu, H;Chen, H;Sun, B;Hyperoside induces apoptosis and inhibits growth in pancreatic cancer via Bcl-2 family and NF-κB signaling pathway both in vitro and in vivoTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine26676634Human umbilical vein endothelial cell line (HUVEC) was obtained from all cells (Shanghai, China) and were cultured in complete medium (HUVEC-004, supplied by AllCells). All cells SaveFull Paper
2016Aizman, I;Holland, WS;Yang, C;Bates, D;αSMA Expression in Large Colonies of Colony-Forming Units-Fibroblast as an Early Predictor of Bone Marrow MSC ExpandabilityCell medicine28003933The three lots that were produced from BM aspirates obtained at AllCells and, coincidentally, from older donors (see Materials and Methods) were all in the slow-growing group; however SaveFull Paper
2016Liu, W;Lu, Y;Chai, X;Liu, X;Zhu, T;Wu, X;Fang, Y;Liu, X;Zhang, X;Antitumor activity of TY-011 against gastric cancer by inhibiting Aurora A, Aurora B and VEGFR2 kinasesJournal of experimental & clinical cancer research : CR27887633Human umbilical vein endothelial cell (HUVEC) obtained from Allcells (Emeryville, CA, USA) were kept in HUVEC basic medium supplemented with 10% FBS (Allcells, Emeryville, CA, SaveFull Paper
2016Han, X;Medeiros, LJ;Zhang, YH;You, MJ;Andreeff, M;Konopleva, M;Bueso-Ramos, CE;High Expression of Human Homologue of Murine Double Minute 4 and the Short Splicing Variant, HDM4-S, in Bone Marrow in Patients With Acute Myeloid Leukemia or Myelodysplastic SyndromeClinical lymphoma, myeloma & leukemia27155969cDNAs from purified normal CD33+ bone marrow myeloid cells, CD34+ hematopoietic progenitor cells, CD71+ normoblasts, and CD19+ circulating peripheral blood B cells were purchased from AllCells, LLC (Emeryville, CA) for reverse transcriptase-polymerasFull Paper
2016Ali, S;Cell Line Cross-Contamination and Accidental Co-CultureJournal of Stem Cell Research & Therapeuticsa https://www.abmgood.com/ Custom-Cell-Authentication-Service.html STR Profiling Analytical Biological Services Inc, USA http://www.absbio.com/products /cellQC/celllineauthen.html STR profiling AllCells, California www.allcells.com - AsteranFull Paper
2016Dou, W;Cheung, S;Gao, C;Xu, C;Xu, L;Wei, J;Detecting table clones and smells in spreadsheetsProceedings of the 2016 24th ACM SIGSOFT International Symposium on Foundations of Software EngineeringTableCheck's clone detection algorithm (Algorithm 1) takes the lookup table and all cells (allCells) Then all cells in this group are removed from allCells (Lines 14-18). (5) The algorithm SaveFull Paper
2016DeWitt, M;Magis, W;Bray, N;Wang, T;Berman, J;Urbinati, F;Muñoz, D;Kohn, D;Walters, M;Carroll, D;Martin, D;Corn, J;Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein ComplexbioRxivCryopreserved WT human mobilized peripheral blood CD34+ HSPCs were purchased from Allcells, Inc. SCD CD34+ HSPCs were prepared by Allcells Inc. from whole blood obtained SaveFull Paper
2016Meader, ES;Understanding the role of miRNA expression in the differentiation of haematopoietic stem cells from pluripotent stem cellsThesisnot been released in spite of several requests, but the sequences of the miRNAs tested are shown in Table 2.4. Small nuclear RNAs RNU44 and RNU48 were chosen as internal controls as they are known to be stably and abundantly expressed across differenFull Paper
2016Tran, R;A microfluidics-based paradigm for clinical lentivector gene transferThesisFrozen human pan T cells were purchased from AllCells (AllCells, LLC., Alameda, CA, USA). Cells were thawed following the AllCells thawing protocol and allowed to recover in RPMI 1640 media supplemented with 10% FBS, L-Glutamine, 25 mM HEPES, and 1%Full Paper
2016Abu Samra, DBK;Molecular Characterization of the Interactions between Vascular Selectins and Glycoprotein Ligands on Human Hematopoietic Stem/Progenitor CellsThesisbone marrow (CD34+ -bone marrow), AML derived bone marrow (from patients with newly diagnosed or relapsed AML; AML bone marrow-CD34+ ) or the mononuclear fraction of whole bone marrow were purchased from ALL CELLS (Alameda, CA, USA; www.allcells.com)Full Paper
2016Ma, L;Targeting Drug Resistance in Chronic Myeloid Leukemia: A DissertationThesisin FBS + 10% DMSO, frozen slowly overnight, and then transferred to liquid nitrogen for long-term storage. All samples were provided in a de-identified manner. Samples from normal donors were purchased from a commercial vendor (AllCells or Lonza)Full Paper
2016Liu, X;Functional and structural study of the AHI-1 SH3 domain, characterization of the BCR-ABL-AHI-1-Dynamin-2 protein complex and investigation of oncogenic roles of dynamin-2 in chronic myeloid leukemiaThesisPrimary CML samples were obtained from newly-diagnosed CP-CML patients before any TKI intervention, and BM cells were obtained from health donors (ALLCELLS, Alameda, CA). The subsequent IM-responders were classified based on the European Leukemia NetFull Paper
2016Ferreira, L;Transcriptional Control of Maternal-Fetal Immune ToleranceThesisPrimary CD4+ T cells were isolated from peripheral blood (Leukopacs, MGH) using RosetteSep CD4+ T cell enrichment cocktail (STEMCELL Technologies). CD34+ cells from G-CSF mobilized peripheral blood were purchased from AllCellsFull Paper
2016Coronado, RE;The effect of decellularized liver matrix on stem cell differentiation into hepatocyte-like cellsThesisBone Marrow Derived Mesenchymal Stromal/Stem Cells: Bone marrow derived mesenchymal stem cells (BM-MSC) were isolated from whole bone marrow aspirates purchased from AllCells Alameda, CA from donors less than 30 years old. The isolation protocol wasFull Paper
2016Gangolli, R;A novel biomimetic scaffold for guided tissue regeneration of the pulp-dentin complexThesisDPSC from AllCells at Passage P5 were used for the study. DPSC-P5 were cultured on T175 flasks using proliferation media with MEM Alpha, 15% FBS and 1% Pen-strep which had been filtered through a 0.2 um filter flask. Culture conditions were maintaineFull Paper
2016Bjornson-Hooper, ZB;Cross-Species Immune System AtlasThesisVenous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human subjects research) or from volunteers from the Stanford community under an IRB-approved protocol. Baboon (Papio hamadryas anubis x Papio haFull Paper
2016Varma, D;Development of a Redox-Crosslinked Cellulose-Based Hydrogel for Nucleus Pulposus Replacement and RepairThesishMSCs were isolated from adult human bone marrow (AllCells, Emeryville, CA) via enriched density gradient centrifugation as previously described (Gupta et al., 2011; Gupta and Nicoll, 2014b). Briefly, an hMSC Enrichment Cocktail (StemCell TechnologieFull Paper
2016Sivajothi, SK;Comparative properties of stromal cells and their role in tissue regenerationThesis2.1 Apheresis Products Non-mobilized PBPC and G-CSF mobilized PBPC were purchased from Allcells LLC (Emeryville, CA): 2.2 Cells and Cell Culture Human bone marrow MSCs were isolated by differential adhesion from bonemarrow aspirate obtained from humaFull Paper
2016Fragiadakis, GK;Profiling Human Immune Variation at the Systems-Level in Clinical Contexts and at Steady-StateThesisBlood Venous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human subjects research) or from volunteers from the Stanford community under an IRB-approved protocol. Antibodies Purified antibodies wereFull Paper
2016Fried, ES;Applications of Proteolipobeads in Biomimetic Cellular Interactions And Lipid Phase SeparationThesisN-Diisopropylethylamine (DIEA), piperidine, carboxymethylcellulose (CMC), and methacrylic anhydride were purchased from Sigma Aldrich (St. Louis, MO). Human mesenchymal stem cells (MSCs) isolated from bone marrow extracted from a 24-year-old male werFull Paper
2016Lucera, MB;Lysine Acetylation and Small Molecule Epigenetic Inhibition Reveal Novel Mechanisms Controlling Cellular Susceptibility to HIV-1 InfectionThesisCD4+ T cells were obtained by leukopheresis from ALLCELLS, LLC. All subjects were healthy control donors negative for HBV, HCV, and HIV. PBMCs were isolated by ficoll gradient separation while CD4+ T cells were purified by adding RosetteSep CD4+ T ceFull Paper
2016Milford, TA;Comparative Studies of TSLP and IL-7 in Normal Early Human Neonatal and Adult B Cell DevelopmentThesisCB was collected according to guidelines approved by the Institutional Review Boards at Children’s Hospital Los Angeles and Loma Linda University. Adult human BM was purchased from AllCells, LLC, Berkeley, CA; the National Disease Research InterchangFull Paper
2016Richter, M;Saydaminova, K;Yumul, R;Krishnan, R;Liu, J;Nagy, EE;Singh, M;Izsvák, Z;Cattaneo, R;Uckert, W;Palmer, D;Ng, P;Haworth, KG;Kiem, HP;Ehrhardt, A;Papayannopoulou, T;Lieber, A;In vivo transduction of primitive mobilized hematopoietic stem cells after intravenous injection of integrating adenovirus vectorsBlood27554082Human CD34+-enriched cells from G-CSF mobilized normal donors were obtained from the Fred Hutchinson Cancer Research Center Cell Processing Core Facility and cultured as described previously.8 Colony-forming unit (CFU) assays were performed using ColFull Paper
2016Castillo, J;Raisner, R;Gascoigne, K;Dos Santos, G;Clarke, E;Huang, A;Lackner, M;Mounir, Z;Abstract 4516: Evaluating the chromatin state as a predictive biomarker for BET inhibitor sensitivity in hematological malignanciesCancer Research1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++2ReachBio, WA.++2ReachBio, WA.++1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++1Genentech, South San FranciFull Paper
2016Ferguson, GD;Delgado, M;Plantevin-Krenitsky, V;Jensen-Pergakes, K;Bates, RJ;Torres, S;Celeridad, M;Brown, H;Burnett, K;Nadolny, L;Tehrani, L;Packard, G;Pagarigan, B;Haelewyn, J;Nguyen, T;Xu, L;Tang, Y;Hickman, M;Baculi, F;Pierce, S;Miyazawa, K;Jackson, P;Chamberlain, P;LeBrun, L;Xie, W;Bennett, B;Blease, K;A Novel Triazolopyridine-Based Spleen Tyrosine Kinase Inhibitor That Arrests Joint InflammationPloS one26756335ature in the dark. Fluorescence readings at 460 nm and 530 nm were taken using the Flexstation II (Molecular Devices). Myeloid colony forming assays to assess Jak2 cellular activity were conducted at ReachBio (Seattle, WA). Ramos NFAT β-lactamase reFull Paper
2016Hariharan, S;STUDY OF GROWTH OF STRESS ERYTHROID PROGENITORS FROM PERIPHERAL BLOOD MONONUCLEAR CELLS IN MURINE AND HUMAN ANEMIC MODEL SYSTEMSThesisThe control Peripheral Blood samples were obtained from ReachBio Labs. The PBMCs were then isolated from peripheral blood using Histopaque 1077 using density gradient. The SaveFull Paper
2016Holzapfel, N;Investigating the Role of the RNA-Binding Protein MUSASHI-2 (MSI2) in Normal Hematopoiesis and LeukemiaThesisAML*CFU%assays%were%performed%in%semi*solid%ColonyGel%media%(Human%Complete% Media% 1102;% reachBio)% with% flow*sorted% GFP+%MSI2% knockdown% AML% cells.% 10000% GFP+%cells%were%plated%in%duplicate%in%each%well%of%a%24*well%plate%and%loose%colonies%Full Paper
2015Sun, LL;Ellerman, D;Mathieu, M;Hristopoulos, M;Chen, X;Li, Y;Yan, X;Clark, R;Reyes, A;Stefanich, E;Mai, E;Young, J;Johnson, C;Huseni, M;Wang, X;Chen, Y;Wang, P;Wang, H;Dybdal, N;Chu, YW;Chiorazzi, N;Scheer, JM;Junttila, T;Totpal, K;Dennis, MS;Ebens, AJ;Anti-CD20/CD3 T cell-dependent bispecific antibody for the treatment of B cell malignanciesSci Transl Med25972002We have obtained 40 ml of whole blood collected in Streck tubes, separated into plasma and extracted cfRNA samples from two Stage IV Prostate cancer patients and three healthy controls from Conversant Bio and generated cfRNA sequencing data with UMIsFull Paper
2015Li, L;Lyu, X;Hou, C;Takenaka, N;Nguyen, HQ;Ong, CT;Cubeñas-Potts, C;Hu, M;Lei, EP;Bosco, G;Qin, ZS;Corces, VG;Widespread rearrangement of 3D chromatin organization underlies polycomb-mediated stress-induced silencingMol. Cell25818644We thank Drs. John T. Lis for providing antibodies against RNAPII, and Vincent Pirrotta and Richard Jones for providing antibodies against Pc. We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology, and specialFull Paper
2015Bhate, A;Parker, DJ;Bebee, TW;Ahn, J;Arif, W;Rashan, EH;Chorghade, S;Chau, A;Lee, JH;Anakk, S;Carstens, RP;Xiao, X;Kalsotra, A;ESRP2 controls an adult splicing programme in hepatocytes to support postnatal liver maturationNat Commun26531099... RI digested amplified PCR products for Esrp2 cDNA in pDP19 vector (Ambion). Sense and anti-sense RNA strands were generated as previously described43. In situ hybridization for Esrp2 was performed by Phylogeny Inc. (Columbus, Ohio.) The C57BL/6 mFull Paper
2015Weckselblatt, B;Hermetz, KE;Rudd, MK;Unbalanced translocations arise from diverse mutational mechanisms including chromothripsisGenome Res.26070663We used Agilent SureSelect Target Enrichment to pull down 40-kb regions around breakpoints fine-mapped by custom array CGH. SureSelect followed by Illumina HiSeq sequencing was performed at HudsonAlpha Genomic Services Laboratory, and sequence analysFull Paper
2015Jagannathan, S;Abdel-Malek, MA;Malek, E;Vad, N;Latif, T;Anderson, KC;Driscoll, JJ;Pharmacologic screens reveal metformin that suppresses GRP78-dependent autophagy to enhance the anti-myeloma effect of bortezomibLeukemia26108695Eagle's medium. Bone marrow aspirates were obtained after approval institutional review board or from ConversantBio (Huntsville, AL, USA) and CD138 + cells then isolated (Miltenyi Biotec, San Diego, CA, USA). Side populationFull Paper
2015Jagannathan, S;Vad, N;Vallabhapurapu, S;Vallabhapurapu, S;Anderson, KC;Driscoll, JJ;MiR-29b replacement inhibits proteasomes and disrupts aggresome+autophagosome formation to enhance the antimyeloma benefit of bortezomibLeukemia25234165(c) Effect of control or miR-29b on myeloma patient tumor cells. CD138 + PCs from bone marrow (BM; ConversantBio, Huntsville, AL, USA) were isolated using microbead-positive selection (Miltenyi Biotec, Auburn, CA, USA)Full Paper
2015Blume-Jensen, P;Berman, DM;Rimm, DL;Shipitsin, M;Putzi, M;Nifong, TP;Small, C;Choudhury, S;Capela, T;Coupal, L;Ernst, C;Hurley, A;Kaprelyants, A;Chang, H;Giladi, E;Nardone, J;Dunyak, J;Loda, M;Klein, EA;Magi-Galluzzi, C;Latour, M;Epstein, JI;Kantoff, P;Saad, F;Development and clinical validation of an in situ biopsy-based multimarker assay for risk stratification in prostate cancerClin. Cancer Res.25733599To develop a robust assay, multiple institutions were recruited representing typical North American patient cohorts (Table 1): Urology Austin, Chesapeake Urology Associates, Cleveland Clinic, Michigan Urology, and Folio Biosciences. Biopsy sample incFull Paper
2015Garcia-Manero, G;Khoury, HJ;Jabbour, E;Lancet, J;Winski, SL;Cable, L;Rush, S;Maloney, L;Hogeland, G;Ptaszynski, M;Calvo, MC;Bohannan, Z;List, A;Kantarjian, H;Komrokji, R;A phase I study of oral ARRY-614, a p38 MAPK/Tie2 dual inhibitor, in patients with low or intermediate-1 risk myelodysplastic syndromesClin. Cancer Res.25480830... s (Aurora, CO) proprietary CellMap customized algorithm for p-p38 and CC3, respectively. Age-defined FFPE bone marrow biopsies from apparently healthy subjects (60–74 years old) were obtained from Folio Biosciences (Columbus, OH). Plasma was isoFull Paper
2015Grabek, KR;Diniz Behn, C;Barsh, GS;Hesselberth, JR;Martin, SL;Enhanced stability and polyadenylation of select mRNAs support rapid thermogenesis in the brown fat of a hibernatorElife25626169Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, United States.++Department of Applied Math and Statistics, Colorado School of Mines, Golden, United States.++Department of Research, HudsonAlpha InstitutFull Paper
2015Li, C;Gowan, S;Anil, A;Beck, BH;Thongda, W;Kucuktas, H;Kaltenboeck, L;Peatman, E;Discovery and validation of gene-linked diagnostic SNP markers for assessing hybridization between Largemouth bass (Micropterus salmoides) and Florida bass (M. floridanus)Mol Ecol Resour25047482; of 110-140 nm. After KAPA quantitation and dilution, based on included DNA standards (1-6), the libraries were sequenced in a single lane on an Illumina HiSeq 2000 instrument with 100 bp paired‐end (PE) reads at HudsonAlpha Genomic Services Lab (HFull Paper
2015Nguyen, D;Rubinstein, L;Takebe, N;Miele, L;Tomaszewski, JE;Ivy, P;Doroshow, JH;Yang, SX;Notch1 phenotype and clinical stage progression in non-small cell lung cancerJ Hematol Oncol25653136Lung cancer tissue microarrays and clinicopathological data were obtained from Cybrdi, Incorporation (Rockville, MD), and FOLIO Biosciences (Powell, OH). Approval of the biomarker study on de-identified human tissues was obtained from the Office of HFull Paper
2015Huynh, H;Hao, HX;Chan, SL;Chen, D;Ong, R;Soo, KC;Pochanard, P;Yang, D;Ruddy, D;Liu, M;Derti, A;Balak, MN;Palmer, MR;Wang, Y;Lee, BH;Sellami, D;Zhu, AX;Schlegel, R;Huang, A;Loss of Tuberous Sclerosis Complex 2 (TSC2) Is Frequent in Hepatocellular Carcinoma and Predicts Response to mTORC1 Inhibitor EverolimusMol. Cancer Ther.25724664HCC cell lines and patient-derived xenografts were collected, fixed with formalin and processed for paraffin embedding. HCC tissue microarrays were purchased from BioChain (catalog # Z7020056 and Z7020057, unrelated HCC TMAs) and FolioBio (catalog# AFull Paper
2015Mezache, L;Paniccia, B;Nyinawabera, A;Nuovo, GJ;Enhanced expression of PD L1 in cervical intraepithelial neoplasia and cervical cancersMod. Pathol.26403783and cervical cancers Louisa Mezache 1 Bernard Paniccia 1 Angelique Nyinawabera 1 Gerard Nuovo 1,2 Phylogeny Inc. 1 , Powell, Ohio and OSU Comprehensive Cancer Center 2 , Columbus Ohio Page 2. 2 - Mezache et al Direct all correspondence to: Gerard J NFull Paper
2015Shen, J;Putt, KS;Visscher, DW;Murphy, L;Cohen, C;Assessment of folate receptor-β expression in human neoplastic tissuesOncotarget... from US Biomax (Rockville, MD), and v) a breast tumor microarray (ARY-HH0056), a brain glioma tumor microarray (ARY-HH0138) and an endometrial carcinoma progression tumor microarray (ARY-HH0211) from Folio Biosciences (Columbus, OH). Oregon greenFull Paper
2015Azad, AK;Rajaram, MV;Metz, WL;Cope, FO;Blue, MS;Vera, DR;Schlesinger, LS;γ-Tilmanocept, a New Radiopharmaceutical Tracer for Cancer Sentinel Lymph Nodes, Binds to the Mannose Receptor (CD206)J. Immunol.26202986We thank Phylogeny, Inc. (Powell, OH) for sectioning and processing the lymph node tissues. We also acknowledge the support of the Campus Microscopy and Imaging Facility of The Ohio State University (Columbus, OH) for confocal microscopy.Full Paper
2015Waight, JD;Takai, S;Marelli, B;Qin, G;Hance, KW;Zhang, D;Tighe, R;Lan, Y;Lo, KM;Sabzevari, H;Hofmeister, R;Wilson, NS;Cutting edge: epigenetic regulation of Foxp3 defines a stable population of CD4+ regulatory T cells in tumors from mice and humansJ. Immunol.25548231Mouse tumor cells were isolated as described previously (12). Human tumor specimens were collected before therapy and purchased from Conversant Bio. Healthy donor PBMCs (Research Blood Components) were isolated using Ficoll-Paque density gradient. SaFull Paper
2015Li, Y;Kocot, KM;Schander, C;Santos, SR;Thornhill, DJ;Halanych, KM;Mitogenomics reveals phylogeny and repeated motifs in control regions of the deep-sea family Siboglinidae (Annelida)Mol. Phylogenet. Evol.25721539Mitogenomics reveals phylogeny and repeated motifs in control regions of the deep-sea family Siboglinidae (Annelida)Full Paper
2015Cheng, A;Hernandez, N;Li, M;San Jose Hinahon, C;Magdaleno, S;Abstract 4755: Maximize genomic analysis through dual recovery of DNA and RNA from the same clinical sampleMolecular and Cellular Biologyvariant, indel data, RNA fusion detection and expression data. MATERIALS AND METHODS FFPE surgical resection and FNA blocks were purchased from Folio Biosciences. Age of blocks ranged from 1992- 2011 with various tumor stages. One 4um section mountedFull Paper
2015Cunningham, CB;Ji, L;Wiberg, RA;Shelton, J;McKinney, EC;Parker, DJ;Meagher, RB;Benowitz, KM;Roy-Zokan, EM;Ritchie, MG;Brown, SJ;Schmitz, RJ;Moore, AJ;The Genome and Methylome of a Beetle with Complex Social Behavior, Nicrophorus vespilloides (Coleoptera: Silphidae)Genome Biol Evol26454014Testing for conserved and novel mechanisms underlying phenotypic evolution requires a diversity of genomes available for comparison spanning multiple independent lineages. For example, complex social behavior in insects has been investigated primarilFull Paper
2015Yamaguchi, SI;Ueki, A;Sugihara, E;Onishi, N;Yaguchi, T;Kawakami, Y;Horiuchi, K;Morioka, H;Matsumoto, M;Nakamura, M;Muto, A;Toyama, Y;Saya, H;Shimizu, T;Synergistic antiproliferative effect of imatinib and adriamycin in platelet-derived growth factor receptor-expressing osteosarcoma cellsCancer Sci.25940371A lung tissue section was randomly obtained from each lobe. Human OS tissue array. An array of human OS specimens was obtained from Folio Biosciences (Powell, OH, USA) and was subjected to immunohistochemical staining for PDGFRβFull Paper
2015Zemskova, MY;Song, JH;Cen, B;Cerda-Infante, J;Montecinos, VP;Kraft, AS;Regulation of prostate stromal fibroblasts by the PIM1 protein kinaseCell. Signal.25451079scores. P values were calculated by t test and represent the probability of no difference between PIM1 positive cells in the stroma of normal and cancer tissues. (C) IHC staining of PIM1 using a TMA (Folio Bioscience). PIM1Full Paper
2015Ergin, A;Großerüschkamp, F;Theisen, O;Gerwert, K;Remiszewski, S;Thompson, CM;Diem, M;A method for the comparison of multi-platform spectral histopathology (SHP) data setsAnalyst25664352M. Miljković, et al., Spectral Cytopathology: new aspects of data collection, manipulation and confounding effects, Analyst, 2013, 138, 3975-3982 RSC . foliobio.com. Deparaffin Protocol. Available from: http://foliobio.com/media/pdf/Deparaffin%20protFull Paper
2015Steffen, MM;Belisle, BS;Watson, SB;Boyer, GL;Bourbonniere, RA;Wilhelm, SW;Metatranscriptomic evidence for co-occurring top-down and bottom-up controls on toxic cyanobacterial communitiesAppl. Environ. Microbiol.25662977... using the Epicentre Ribo-Zero rRNA removal kit (Epicentre, Madison, WI, USA). Secondary quality analysis and sequencing were done on the Illumina HiSeq platform for 50-bp paired-end sequencing by the HudsonAlpha Genomic Services Laboratory (HuntsFull Paper
2015Van Bortle, K;Peterson, AJ;Takenaka, N;O'Connor, MB;Corces, VG;CTCF-dependent co-localization of canonical Smad signaling factors at architectural protein binding sites in D. melanogasterCell Cycle26125535llumina adaptors (Illumina Cat# PE-102-1001) were titrated according to prepared DNA ChIP sample concentration and ligated with T4 ligase (NEB Cat# M0202S). Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (NEFull Paper
2015Ritchie, SA;Chitou, B;Zheng, Q;Jayasinghe, D;Jin, W;Mochizuki, A;Goodenowe, DB;Pancreatic cancer serum biomarker PC-594: Diagnostic performance and comparison to CA19-9World J. Gastroenterol.26074698MATERIALS AND METHODS. Sample cohort. PaC (n = 84) and control (n = 99) serum samples were obtained from Conversant Bio (www.conversantbio.com). All patients signed informed consents, and samples were collectedFull Paper
2015Premkumar, DR;Jane, EP;Pollack, IF;Cucurbitacin-I inhibits Aurora kinase A, Aurora kinase B and survivin, induces defects in cell cycle progression and promotes ABT-737-induced cell death in a caspase-independent manner in malignant human glioma cellsCancer Biol. Ther.25482928We also obtained primary glioma cells from Conversant Biologics (Huntsville, AL). Established malig- nant human glioma cell lines such as T98G, A172 and LN229 were obtained from the American Type Culture Collection (Manassas, VA)Full Paper
2015Tehranchian, P;Norsworthy, JK;Nandula, V;McElroy, S;Chen, S;Scott, RC;First report of resistance to acetolactate-synthase-inhibiting herbicides in yellow nutsedge (Cyperus esculentus): confirmation and characterizationPest Manag. Sci.25307777To identify potential target-site mutations, massively parallel sequencing using the Illumina HiSeq (Illumina, San Diego, CA; http://www.illumina.com/) platform was utilized in lieu of traditional short-read capillary sequencing using the polymeraseFull Paper
2015Campos, AH;Schreeder, M;Parry-Jones, A;Abdelhafiz, AS;Larson, D;Pruetz, B;Geddes, T;Salman, A;Lazaris, A;Addressing the Challenge of Financial Sustainability in BiobankingBiopreserv Biobank26697907Marshall Schreeder. Chief Executive Officer, Co-Founder, Conversant Bio, Huntsville, Alabama Conversant Bio, Huntsville, Alabama. Conversant Bio was founded in 2006 because the founders knew that the drug discovery research model was brokenFull Paper
2015Chen, S;McElroy, JS;Flessner, ML;Dane, F;Utilizing next-generation sequencing to study homeologous polymorphisms and herbicide-resistance-endowing mutations in Poa annua acetolactate synthase genesPest Manag. Sci.25180862Detection of single nucleotide polymorphisms (SNPs) related to herbicide resistance in non-model polyploid weed species is fraught with difficulty owing to the gene duplication and lack of reference sequences. Our research seeks to overcome these obsFull Paper
2015Cui, J;Xu, J;Zhang, S;Wang, K;Jiang, Y;Mahboob, S;Al-Ghanim, KA;Xu, P;Transcriptional Profiling Reveals Differential Gene Expression of Amur Ide (Leuciscus waleckii) during Spawning MigrationInt J Mol Sci26096003... nt Technologies, Santa Clara, CA, USA). Equal amounts of high quality RNA samples from the liver were then pooled for RNA-Seq analysis. RNA-Seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2015Baker, GM;Murphy, T;Block, T;Nguyen, D;Lynch, FJ;Development and validation of an immunohistochemistry assay to assess glucocorticoid receptor expression for clinical trials of mifepristone in breast cancerCancer Manag Res26673410Tiffany Murphy. 2 QualTek Molecular Laboratories, Newtown, PA, USA. Find articles by Tiffany Murphy. Thaddeus Block Dat Nguyen. 3 Corcept Therapeutics, Menlo Park, CA, USA. Find articles by Dat Nguyen. Frank J Lynch. 2 QualTek Molecular LaboratorieFull Paper
2015Zisis, K;Piper, M;Henderson, MK;The 2015 ISBER Annual Meeting & Exhibits in Phoenix, Arizona, USA: bridging the canyon-connecting biobank communities through innovations in global health, research and environmental preservationBiopreserv Biobank25686051This year's session focused on the business aspects of biobank planning, and included Marshall Schreeder's (ConversantBIO) perspective from a commer- cial biobank and Clive Green's (AstraZeneca) business planning for a pharmaceutical biobankFull Paper
2015Zhao, H;Li, C;Beck, BH;Zhang, R;Thongda, W;Davis, DA;Peatman, E;Impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings, Ictalurus punctatusFish Shellfish Immunol.26164837RNA quality of each sample was measured on an Agilent 2100 Bioanalyzer using the RNA Nano Bioanalysis chip. RNA-seq library preparation was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA)Full Paper
2015Zhu, J;Li, C;Ao, Q;Tan, Y;Luo, Y;Guo, Y;Lan, G;Jiang, H;Gan, X;Trancriptomic profiling revealed the signatures of acute immune response in tilapia (Oreochromis niloticus) following Streptococcus iniae challengeFish Shellfish Immunol.26117728; For each timepoint, equal amounts of RNA from the three replicates were pooled for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA)Full Paper
2015Foster, KA;Jane, EP;Premkumar, DR;Morales, A;Pollack, IF;NVP-BKM120 potentiates apoptosis in tumor necrosis factor-related apoptosis-inducing ligand-resistant glioma cell lines via upregulation of Noxa and death receptor 5Int. J. Oncol.26044191U87 was obtained from American Type Culture Collection (Manasas, VA, USA) and LNZ308 was kindly provided by Dr Nicolas de Tribolet (Switzerland). We obtained primary GBM cells from Conversant Biologics (Huntsville, AL, USA)Full Paper
2015Baxter, A;Thain, S;Banerjee, A;Haswell, L;Parmar, A;Phillips, G;Minet, E;Targeted omics analyses, and metabolic enzyme activity assays demonstrate maintenance of key mucociliary characteristics in long term cultures of reconstituted human airway epitheliaToxicol In Vitro25863282The 180 PBS was collected and stored at A80 °C. Sputum from 10 non- 181 smokers (Sofia Bio, Sofia, Bulgaria, study code SB-SC_v1) and 182 MucilAir™ ASL were thawed, centrifuged and filtered using a 183 0.2 lm filter plate (PALL Corporation, Port WashFull Paper
2015Marshall, DC;Lyman, SK;McCauley, S;Kovalenko, M;Spangler, R;Liu, C;Lee, M;O'Sullivan, C;Barry-Hamilton, V;Ghermazien, H;Mikels-Vigdal, A;Garcia, CA;Jorgensen, B;Velayo, AC;Wang, R;Adamkewicz, JI;Smith, V;Selective Allosteric Inhibition of MMP9 Is Efficacious in Preclinical Models of Ulcerative Colitis and Colorectal CancerPLoS ONE25961845... h as CRC. Materials and Methods Tissues Fresh-frozen (FF) and formalin fixed and paraffin embedded (FFPE) human tissues were obtained from Cureline, Inc. (Burlingame, CA), Asterand (Detroit, MI), or Folio Biosciences (Powell, OH). Recombinant MFull Paper
2015Hoek, KL;Samir, P;Howard, LM;Niu, X;Prasad, N;Galassie, A;Liu, Q;Allos, TM;Floyd, KA;Guo, Y;Shyr, Y;Levy, SE;Joyce, S;Edwards, KM;Link, AJ;A cell-based systems biology assessment of human blood to monitor immune responses after influenza vaccinationPLoS ONE25706537Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, 37232, United States of America.++Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, 37232, United StatesFull Paper
2015Kalinsky, K;Mayer, JA;Xu, X;Pham, T;Wong, KL;Villarin, E;Pircher, TJ;Brown, M;Maurer, MA;Bischoff, FZ;Correlation of hormone receptor status between circulating tumor cells, primary tumor, and metastasis in breast cancer patientsClin Transl Oncol25613123Peripheral blood was collected under appropriate third party institution review board approved protocols (Columbia University Medical Center, AdeptBio, ConversantBIO and BioOptions) and delivered to Biocept's CLIA/CAP accredited laboratoryFull Paper
2015Wang, Z;Lebron, JA;Wolf, JJ;Reliable quantification of mRNA in archived formalin-fixed tissue with or without paraffin embeddingJ Pharmacol Toxicol Methods25280797quantify mRNA in archived FFPE and prolonged formalin-fixed tissue. 2. Methods. 2.1. Samples. Four human FFPE liver samples were purchased from Folio Biosciences. Two mouse FFPE liver samples and two mouse formalinFull Paper
2015Rokyta, DR;Wray, KP;McGivern, JJ;Margres, MJ;The transcriptomic and proteomic basis for the evolution of a novel venom phenotype within the Timber Rattlesnake (Crotalus horridus)Toxicon25727380Sequencing and nonnormalized cDNA library preparation for our two adults were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially aFull Paper
2015Pawar, H;Srikanth, SM;Kashyap, MK;Sathe, G;Chavan, S;Singal, M;Manju, HC;Kumar, KV;Vijayakumar, M;Sirdeshmukh, R;Pandey, A;Prasad, TS;Gowda, H;Kumar, RV;Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell CarcinomaScientificWorldJournal26788548The ESCC tumors varied from Grade I to Grade III and were obtained from patients in the age group of 36 to 78 years and (ii) FolioBio (Powell, OH; Catalog number ARY-HH0091) consisting of 40 ESCC cases with matched adjacent normal esophageal epitheliFull Paper
2015Krönke, J;Fink, EC;Hollenbach, PW;MacBeth, KJ;Hurst, SN;Udeshi, ND;Chamberlain, PP;Mani, DR;Man, HW;Gandhi, AK;Svinkina, T;Schneider, RK;McConkey, M;Järås, M;Griffiths, E;Wetzler, M;Bullinger, L;Cathers, BE;Carr, SA;Chopra, R;Ebert, BL;Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDSNature26131937uced cells in each condition was assessed after 5 days by flow cytometry. Control cord-blood CD34+ cells were isolated as above. Adult bone marrow CD34+ cells were purchased as single-donor lots from AllCells (Alameda, CA). The number of replicates fFull Paper
2015Hendel, A;Bak, RO;Clark, JT;Kennedy, AB;Ryan, DE;Roy, S;Steinfeld, I;Lunstad, BD;Kaiser, RJ;Wilkens, AB;Bacchetta, R;Tsalenko, A;Dellinger, D;Bruhn, L;Porteus, MH;Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cellsNature biotechnology26121415Cas9-encoding plasmid. Mobilized human peripheral blood CD34+ HSPCs were purchased from AllCells and thawed according to manufacturer’s instructions. CD34+ HSPCs were maintained in X-VIVO 15 (Lonza) supplemented with SCF (100 ng/ml), TPO (100 ng/ml),Full Paper
2015Kotini, AG;Chang, CJ;Boussaad, I;Delrow, JJ;Dolezal, EK;Nagulapally, AB;Perna, F;Fishbein, GA;Klimek, VM;Hawkins, RD;Huangfu, D;Murry, CE;Graubert, T;Nimer, SD;Papapetrou, EP;Functional analysis of a chromosomal deletion associated with myelodysplastic syndromes using isogenic human induced pluripotent stem cellsNature biotechnology25798938CB CD34 + cells were purchased from AllCells. Two to four shRNAs against each of the four genes HIPK2, ATP6V0E2, LUC7L2 and EZH2 and one scramble sequence were inserted in SaveFull Paper
2015Levine, JH;Simonds, EF;Bendall, SC;Davis, KL;Amir, el-AD;Tadmor, MD;Litvin, O;Fienberg, HG;Jager, A;Zunder, ER;Finck, R;Gedman, AL;Radtke, I;Downing, JR;Pe'er, D;Nolan, GP;Data-Driven Phenotypic Dissection of AML Reveals Progenitor-like Cells that Correlate with PrognosisCell26095251For healthy adult controls, cryopreserved healthy BMMCs were purchased from AllCells (Emeryville, CA). All human samples were obtained with informed consent in compliance with SaveFull Paper
2015Moraga, I;Wernig, G;Wilmes, S;Gryshkova, V;Richter, CP;Hong, WJ;Sinha, R;Guo, F;Fabionar, H;Wehrman, TS;Krutzik, P;Demharter, S;Plo, I;Weissman, IL;Minary, P;Majeti, R;Constantinescu, SN;Piehler, J;Garcia, KC;Tuning cytokine receptor signaling by re-orienting dimer geometry with surrogate ligandsCell25728669ficolled cells (32K) from a single healthy donor (donor ID #: 6024, lot#: BM4871) were purchased from Allcells, Alameda, CA 94502. Cells were subsequently treated with DNAase. Milteny CD34 magnetic beads were used to enrich for CD34+ cells withFull Paper
2015Vierstra, J;Reik, A;Chang, KH;Stehling-Sun, S;Zhou, Y;Hinkley, SJ;Paschon, DE;Zhang, L;Psatha, N;Bendana, YR;O'Neil, CM;Song, AH;Mich, AK;Liu, PQ;Lee, G;Bauer, DE;Holmes, MC;Orkin, SH;Papayannopoulou, T;Stamatoyannopoulos, G;Rebar, EJ;Gregory, PD;Urnov, FD;Stamatoyannopoulos, JA;Functional footprinting of regulatory DNANature methods26322838Human mobilized CD34 + cells (adult) were purchased from AllCells. Small-scale mRNA transfections were performed with a BTX device (Harvard Apparatus), using the CD34 + cell SaveFull Paper
2015Bunin, A;Sisirak, V;Ghosh, HS;Grajkowska, LT;Hou, ZE;Miron, M;Yang, C;Ceribelli, M;Uetani, N;Chaperot, L;Plumas, J;Hendriks, W;Tremblay, ML;Häcker, H;Staudt, LM;Green, PH;Bhagat, G;Reizis, B;Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic CellsImmunity26231120PBMC were isolated from healthy adult volunteers by Histopaque density gradient centrifugation; where indicated, pDCs were enriched using the Diamond pDC isolation kit (Miltenyi Biotec) to 95% purity. Human PBMC subsets for expression analysis were pFull Paper
2015Behbehani, GK;Samusik, N;Bjornson, ZB;Fantl, WJ;Medeiros, BC;Nolan, GP;Mass Cytometric Functional Profiling of Acute Myeloid Leukemia Defines Cell-Cycle and Immunophenotypic Properties That Correlate with Known Responses to TherapyCancer discovery26091827Healthy control samples were obtained from Allcells using the same protocol. The exception was healthy donor sample #1; this sample was incubated for approximately 1 hour with IdU SaveFull Paper
2015Casero, D;Sandoval, S;Seet, CS;Scholes, J;Zhu, Y;Ha, VL;Luong, A;Parekh, C;Crooks, GM;Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T cell lineagesNature immunology26502406Anonymous normal human thymi were obtained via the UCLA Translational Pathology Core Laboratory and the CHLA cardiovascular thoracic surgery department. Bone marrow (BM) was obtained from donors via ALLCELLS, Alameda, CA. All tissues were collected aFull Paper
2015Swaminathan, S;Klemm, L;Park, E;Papaemmanuil, E;Ford, A;Kweon, SM;Trageser, D;Hasselfeld, B;Henke, N;Mooster, J;Geng, H;Schwarz, K;Kogan, SC;Casellas, R;Schatz, DG;Lieber, MR;Greaves, MF;Müschen, M;Mechanisms of clonal evolution in childhood acute lymphoblastic leukemiaNature immunology25985233Human CD19 + cord blood B cells were obtained from AllCells. AllCells procures cord blood subsequent to receiving informed consent from its donors. We have approval to use cord SaveFull Paper
2015Pandolfi, A;Stanley, RF;Yu, Y;Bartholdy, B;Pendurti, G;Gritsman, K;Boultwood, J;Chernoff, J;Verma, A;Steidl, U;PAK1 is a therapeutic target in acute myeloid leukemia and myelodysplastic syndromeBlood26170031Poor clinical outcome of acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) has been attributed to failure of current chemotherapeutic regimens to target leukemic SaveFull Paper
2015Patel, V;Balakrishnan, K;Keating, MJ;Wierda, WG;Gandhi, V;Expression of executioner procaspases and their activation by a procaspase-activating compound in chronic lymphocytic leukemia cellsBlood25538042Normal B lymphocytes (freshly isolated negatively selected CD19 + B cells) were purchased from AllCells, and were kept in RPMI-1640 medium with 10% fetal bovine serum (FBS). SaveFull Paper
2015Iwasa, M;Miura, Y;Fujishiro, A;Fujii, S;Sugino, N;Yoshioka, S;Tamura, A;Sato, A;Yokota, A;Kito, K;Ando, A;Hirai, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T;Bortezomib Attenuates Adhesion of B Cell Precursor Acute Lymphoblastic Lleukemia Cells to Bone Marrow Mesenchymal Stromal/Stem Cells Via Regulating SPARC ExpressionBloodWe isolated human BM-MSCs from normal bone marrow samples (AllCells, Emeryville, CA). When Nalm6 cells were co-cultured with human BM-MSCs, they were prone to adhere to BM Save;The prognosis of adults with B cell precursor acute lymphoblastic leukemFull Paper
2015Shenoy, S;Eapen, M;Wu, J;Walters, M;Levine, J;Logan, B;Gersten, I;Kamani, N;A Multicenter Phase II Trial of Unrelated Donor Reduced Intensity Bone Marrow Transplantation for Children with Severe Sickle Cell Disease (SCURT): Results of the Blood and Marrow Transplant Clinical Trials Network (BMT CTN 0601) StudyBloodBackground: Children with severe sickle cell disease (SCD) experience organ damage, poor quality of life, and a risk of premature mortality. Allogeneic bone marrow transplantation (BMT) halts disease progression when stable donor erythFull Paper
2015Krishnamurti, L;Sullivan, K;Kamani, N;Waller, E;Abraham, A;Campigotto, F;Zhang, W;Smith, S;Hassell, K;Decastro, L;Wu, C;Neuberg, D;Walters, M;Results of a Multicenter Pilot Investigation of Bone Marrow Transplantation in Adults with Sickle Cell Disease (STRIDE)BloodBackground: Hematopoietic cell transplantation (HCT) for sickle cell disease (SCD) is potentially curative, yet applied sparingly in adults due in part to limited donor availability and to concerns about safety. We conducted a multicenter pilot cliniFull Paper
2015Cappelli, B;Bernaudin, F;Ruggeri, A;Labopin, M;Volt, F;Simoes, B;Ferster, A;Dupont, S;de la Fuente, J;Dalle, J;Zecca, M;Robin, M;Walters, M;Dhedin, N;Michel, G;Lutz, P;Neven, B;Bertrand, Y;Vannier, J;Ayas, M;Matthes, S;Elayoubi, H;Devergie, A;Kenzey, C;Locatelli, F;Peters, C;Rocha, V;Eapen, M;Gluckman, E;Hematopoietic Stem Cell Transplantation from HLA Identical Sibling Forsickle Cell Disease an International Survey on Behalf of Eurocord-Monacord, EBMT Paediatric Disease Working Party and CIBMTRBloodIntroduction: Hematopoietic stem cell transplantation (HSCT) is, to date, the only curative therapy for sickle cell disease (SCD). However, HSCT is offered to relatively few patients with SCD for a number of reasons including lack of aFull Paper
2015Kanter, J;Walters, M;Hsieh, M;Thompson, A;Krishnamurti, L;Kwiatkowski, J;Kamble, R;von Kalle, C;Kuypers, F;Cavazzana, M;Leboulch, P;Sandler, L;Soni, S;Tisdale, J;Initial Results from Study Hgb-206: A Phase 1 Study Evaluating Gene Therapy By Transplantation of Autologous CD34+ Stem Cells Transduced Ex Vivo with the Lentiglobin BB305 Lentiviral Vector in Subjects with Severe Sickle Cell DiseaseBloodBackground: Hematopoietic stem cell (HSC) gene transfer has the potential to reduce or eliminate the symptoms of severe sickle cell disease (SCD). Previously reported early results in a single subject with severe SCD from the ongoing study HGB-205 suFull Paper
2015Walters, M;Rasko, J;Hongeng, S;Kwiatkowski, J;Schiller, G;Kletzel, M;Ho, P;Vichinsky, E;von Kalle, C;Cavazzana, M;Leboulch, P;Petrusich, A;Soni, S;Thompson, A;Update of Results from the Northstar Study (HGB-204): A Phase 1/2 Study of Gene Therapy for Beta-Thalassemia Major Via Transplantation of Autologous Hematopoietic Stem Cells Transduced Ex-Vivo with a Lentiviral Beta AT87Q-Globin Vector (LentiGlobin BB305 Drug Product)BloodBackground: Hematopoietic stem cell (HSC) gene transfer has the potential to induce globin production and mitigate or eliminate blood transfusions in patients with β-thalassemia major. Previously reported early results in subjects with β-thalassemiaFull Paper
2015Fujishiro, A;Miura, Y;Iwasa, M;Fujii, S;Tamura, A;Sato, A;Yokota, A;Sugino, N;Hirai, H;Ando, A;Ichinohe, T;Maekawa, T;Vitamin K2 Supports Hematopoiesis through Acting on Bone Marrow Mesenchymal Stromal/Stem CellsBlood[Methods] Normal bone marrow (BM) samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA). BM-CD34 + cells were isolated from BM-mononuclear cells Save;[Background] Myelodysplastic syndrome is an intractable disorder chaFull Paper
2015Kikushige, Y;Miyamoto, T;Yuda, J;Jabbarzadeh-Tabrizi, S;Shima, T;Takayanagi, S;Niiro, H;Yurino, A;Miyawaki, K;Takenaka, K;Iwasaki, H;Akashi, K;A TIM-3/Gal-9 Autocrine Stimulatory Loop Drives Self-Renewal of Human Myeloid Leukemia Stem Cells and Leukemic ProgressionCell stem cell26279267summarizes the primary AML patients’ characteristics analyzed in this study. Human adult BM and PB samples were obtained from healthy donors or purchased from AllCells. CB cells were obtained from full-term deliveries (provided by the Kyushu Block ReFull Paper
2015Jeong, JY;Levine, MS;Abayasekara, N;Berliner, N;Laubach, J;Vanasse, GJ;The non-peptide thrombopoietin receptor agonist eltrombopag stimulates megakaryopoiesis in bone marrow cells from patients with relapsed multiple myelomaJournal of hematology & oncology25886818were also obtained from commercial sources (AllCells, Emeryville, CA and Proteogenix, from Lonza (Basel, Switzerland) and AllCells, respectively. Normal human platelets were SaveFull Paper
2015Goldstein, RL;Yang, SN;Taldone, T;Chang, B;Gerecitano, J;Elenitoba-Johnson, K;Shaknovich, R;Tam, W;Leonard, JP;Chiosis, G;Cerchietti, L;Melnick, A;Pharmacoproteomics identifies combinatorial therapy targets for diffuse large B cell lymphomaThe Journal of clinical investigation26529251HEPES, and penicillin/streptomycin. CD19+ cells were purchased from AllCells. PU-H71 was synthesized as previously reported (5). Ibrutinib was provided by Pharmacyclics. R406, BKM120, and sotrastaurin were purchased from Selleck Chemicals. MI-2 was pFull Paper
2015Ma, SD;Xu, X;Plowshay, J;Ranheim, EA;Burlingham, WJ;Jensen, JL;Asimakopoulos, F;Tang, W;Gulley, ML;Cesarman, E;Gumperz, JE;Kenney, SC;LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesisThe Journal of clinical investigation25485679Alternatively, commercially purchased CD34-depleted human cord blood mononuclear cells (AllCells LLC, catalog no. CB117) were mock-infected or infected with WT EBV or LMP1-KO EBV in vitro for 1.5 hours, after which 12 × 106-25 × 106 cells were injectFull Paper
2015Chichili, GR;Huang, L;Li, H;Burke, S;He, L;Tang, Q;Jin, L;Gorlatov, S;Ciccarone, V;Chen, F;Koenig, S;Shannon, M;Alderson, R;Moore, PA;Johnson, S;Bonvini, E;A CD3xCD123 bispecific DART for redirecting host T cells to myelogenous leukemia: preclinical activity and safety in nonhuman primatesScience translational medicine26019218Cryopreserved AML patient primary PBMCs and bone marrow mononuclear cells (BMMNCs) were purchased from AllCells, LLC, which obtains biological specimens from a donor SaveFull Paper
2015Lee, ST;Muench, MO;Fomin, ME;Xiao, J;Zhou, M;de Smith, A;Martín-Subero, JI;Heath, S;Houseman, EA;Roy, R;Wrensch, M;Wiencke, J;Metayer, C;Wiemels, JL;Epigenetic remodeling in B-cell acute lymphoblastic leukemia occurs in two tracks and employs embryonic stem cell-like signaturesNucleic acids research25690899l, pre-B-II cells for whole genome bisulfite sequencing (WGBS) were purified. Purified normal mature B-cells, T-cells, regulatory T-cells, NK-cells, monocytes and granulocytes were purchased from the Allcells Corp. DNAs were extracted as described abFull Paper
2015Jung, N;Dai, B;Gentles, AJ;Majeti, R;Feinberg, AP;An LSC epigenetic signature is largely mutation independent and implicates the HOXA cluster in AML pathogenesisNature communications26444494n cancer biology using large data sets. Methods Human samples Fresh human bone marrow mononuclear cells (BMMCs) from healthy donors (2 × 108 cells per donor, Catalogue#: ABM006) were purchased from ALLCELLS (Emeryville, CA). A CD34+ cell-enrichmentFull Paper
2015Naka, K;Jomen, Y;Ishihara, K;Kim, J;Ishimoto, T;Bae, EJ;Mohney, RP;Stirdivant, SM;Oshima, H;Oshima, M;Kim, DW;Nakauchi, H;Takihara, Y;Kato, Y;Ooshima, A;Kim, SJ;Dipeptide species regulate p38MAPK-Smad3 signalling to maintain chronic myelogenous leukaemia stem cellsNature communications26289811Viable BM MNCs from three human patients with chronic phase CML were purchased from Allcells (#06-255, #06-620 and #147742, Alameda, CA, USA). We stained these cells with SaveFull Paper
2015Buchner, M;Park, E;Geng, H;Klemm, L;Flach, J;Passegué, E;Schjerven, H;Melnick, A;Paietta, E;Kopanja, D;Raychaudhuri, P;Müschen, M;Identification of FOXM1 as a therapeutic target in B-cell lineage acute lymphoblastic leukaemiaNature communications25753524nslocation of oestrogen receptor fusion proteins, 4-OHT was added at a concentration of 500 nM. Cell sorting For magnetic bead sorting, peripheral blood or BM mononuclear cells were purchased from AllCells LLC and CD19-enrichment was performed by maFull Paper
2015Zhang, W;Wray, LS;Rnjak-Kovacina, J;Xu, L;Zou, D;Wang, S;Zhang, M;Dong, J;Li, G;Kaplan, DL;Jiang, X;Vascularization of hollow channel-modified porous silk scaffolds with endothelial cells for tissue regenerationBiomaterials25934280HUVECs were purchased from AllCells biology technology limited company (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth SaveFull Paper
2015Coquery, CM;Loo, WM;Wade, NS;Bederman, AG;Tung, KS;Lewis, JE;Hess, H;Erickson, LD;BAFF regulates follicular helper t cells and affects their accumulation and interferon-γ production in autoimmunityArthritis & rheumatology (Hoboken, N.J.)25385309Additional blood from SLE patients was purchased from AllCells to increase the range of SLE Disease Activity Index (SLEDAI) scores 24. AllCells used the same protocol and materials SaveFull Paper
2015Stephenson, ST;Brown, LA;Helms, MN;Qu, H;Brown, SD;Brown, MR;Fitzpatrick, AM;Cysteine oxidation impairs systemic glucocorticoid responsiveness in children with difficult-to-treat asthmaThe Journal of allergy and clinical immunology25748343Cell culture experiments were performed with THP-1 monocytes (ATCC®, Manassas, VA), MM.1S B lymphoblasts (ATCC®) and primary PBMCs from healthy donors (AllCells, Alameda, CA). Reduced and oxidized conditions were created by adding 300 μL of 10 mM cysFull Paper
2015Karjalainen, K;Jaalouk, DE;Bueso-Ramos, C;Bover, L;Sun, Y;Kuniyasu, A;Driessen, WH;Cardó-Vila, M;Rietz, C;Zurita, AJ;O'Brien, S;Kantarjian, HM;Cortes, JE;Calin, GA;Koivunen, E;Arap, W;Pasqualini, R;Targeting IL11 Receptor in Leukemia and Lymphoma: A Functional Ligand-Directed Study and Hematopathology Analysis of Patient-Derived SpecimensClinical cancer research : an official journal of the American Association for Cancer Research25779950Normal blood and bone marrow samples were commercially obtained (AllCells). Cells were maintained in humidified hypoxia chambers (HeraCell 150; Thermo Electron Corporation) SaveFull Paper
2015Morelli, E;Leone, E;Cantafio, ME;Di Martino, MT;Amodio, N;Biamonte, L;Gullà, A;Foresta, U;Pitari, MR;Botta, C;Rossi, M;Neri, A;Munshi, NC;Anderson, KC;Tagliaferri, P;Tassone, P;Selective targeting of IRF4 by synthetic microRNA-125b-5p mimics induces anti-multiple myeloma activity in vitro and in vivoLeukemia25987254Biosciences, CA, USA); lentiviral particles were produced and transduced as previously described.19 RNA extraction and qRT-PCR. RNA samples of healthy donors BM-derived plasma cells were purchased (AllCells LLC, Alameda, CA, USA). Total RNA extractiFull Paper
2015Holm, F;Hellqvist, E;Mason, CN;Ali, SA;Delos-Santos, N;Barrett, CL;Chun, HJ;Minden, MD;Moore, RA;Marra, MA;Runza, V;Frazer, KA;Sadarangani, A;Jamieson, CH;Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewalProceedings of the National Academy of Sciences of the United States of America26621726from patients at the University of California, San Diego and the University of Toronto Health Network according to Institutional Review Board-approved informed consent protocols. Human CD34+ cord blood cells and NPB cells were purchased from AllCellsFull Paper
2015Sundaravel, S;Duggan, R;Bhagat, T;Ebenezer, DL;Liu, H;Yu, Y;Bartenstein, M;Unnikrishnan, M;Karmakar, S;Liu, TC;Torregroza, I;Quenon, T;Anastasi, J;McGraw, KL;Pellagatti, A;Boultwood, J;Yajnik, V;Artz, A;Le Beau, MM;Steidl, U;List, AF;Evans, T;Verma, A;Wickrema, A;Reduced DOCK4 expression leads to erythroid dysplasia in myelodysplastic syndromesProceedings of the National Academy of Sciences of the United States of America26578796CD34 + HSCs and progenitor cells were purified from G-mobilized peripheral blood of healthy donors purchased from AllCells, Inc. using a CliniMACS (Miltenyi Biotec, Inc.) instrument. SaveFull Paper
2015Yea, K;Zhang, H;Xie, J;Jones, TM;Lin, CW;Francesconi, W;Berton, F;Fallahi, M;Sauer, K;Lerner, RA;Agonist antibody that induces human malignant cells to kill one anotherProceedings of the National Academy of Sciences of the United States of America26487683The one patient with M5 differentiated cells had been treated with Ara-C for 4 mo before collection of cells; the other six patients were untreated. Samples of leukemic cells obtained by BM aspiration or from PB were purchased from the AllCells corpoFull Paper
2015Lovat, F;Fassan, M;Gasparini, P;Rizzotto, L;Cascione, L;Pizzi, M;Vicentini, C;Balatti, V;Palmieri, D;Costinean, S;Croce, CM;miR-15b/16-2 deletion promotes B-cell malignanciesProceedings of the National Academy of Sciences of the United States of America26324892Technologies) according to the manufacturer’s protocol. Real-time PCR experiments were carried out using miR-15b and pri-miR-15b assays for real-time PCR (Applied Biosystems) according to the manufacturer’s protocol. Control human cord blood CD19+ BFull Paper
2015Mori, Y;Chen, JY;Pluvinage, JV;Seita, J;Weissman, IL;Prospective isolation of human erythroid lineage-committed progenitorsProceedings of the National Academy of Sciences of the United States of America26195758Antibodies, Cell Staining, and Sorting. The sorting procedures for HSCs and myeloid progenitor populations that we previously reported (20) were slightly modified. In brief, bone marrow mononuclear cells, purchased from AllCells, were first stained wFull Paper
2015Li, Y;Rogoff, HA;Keates, S;Gao, Y;Murikipudi, S;Mikule, K;Leggett, D;Li, W;Pardee, AB;Li, CJ;Suppression of cancer relapse and metastasis by inhibiting cancer stemnessProceedings of the National Academy of Sciences of the United States of America25605917Human CD34 + bone marrow cells (AllCells) were cultured in MethoCult media to form colonies as described by the manufacturer (Stemcell Technologies). BBI608 (2-Acetyl-4H,9H- SaveFull Paper
2015Kuramitsu, M;Okuma, K;Yamochi, T;Sato, T;Sasaki, D;Hasegawa, H;Umeki, K;Kubota, R;Sobata, R;Matsumoto, C;Kaneko, N;Naruse, I;Yamagishi, M;Nakashima, M;Momose, H;Araki, K;Mizukami, T;Mizusawa, S;Okada, Y;Ochiai, M;Utsunomiya, A;Koh, KR;Ogata, M;Nosaka, K;Uchimaru, K;Iwanaga, M;Sagara, Y;Yamano, Y;Satake, M;Okayama, A;Mochizuki, M;Izumo, S;Saito, S;Itabashi, K;Kamihira, S;Yamaguchi, K;Watanabe, T;Hamaguchi, I;Standardization of Quantitative PCR for Human T-Cell Leukemia Virus Type 1 in Japan: a Collaborative StudyJournal of clinical microbiology26292315PBMCs were purchased from AllCells Inc. (Alameda, CA, USA). TL-Om1 cells were in RPMI 1640 supplemented with 10% FBS at 37C according to the protocol provided by AllCells Inc. SaveFull Paper
2015Kuramitsu, M;Okuma, K;Yamagishi, M;Yamochi, T;Firouzi, S;Momose, H;Mizukami, T;Takizawa, K;Araki, K;Sugamura, K;Yamaguchi, K;Watanabe, T;Hamaguchi, I;Identification of TL-Om1, an adult T-cell leukemia (ATL) cell line, as reference material for quantitative PCR for human T-lymphotropic virus 1Journal of clinical microbiology25502533PBMCs were kindly provided by the Japanese Red Cross or purchased from AllCells (Alameda, CA, USA). TL-Om1 cells, an ATL-derived cell line established by Sugamura et al. (17), were maintained in RPMI 1640 (Sigma, St. Louis, MO, USA) containing 10% feFull Paper
2015Lancet, JE;Roboz, GJ;Cripe, LD;Michelson, GC;Fox, JA;Leavitt, RD;Chen, T;Hawtin, R;Craig, AR;Ravandi, F;Maris, MB;Stuart, RK;Karp, JE;A phase 1b/2 study of vosaroxin in combination with cytarabine in patients with relapsed or refractory acute myeloid leukemiaHaematologica25381131clinical samples, induction of pDNA-PKcs following a 2 h exposure to vosaroxin was examined. PBMC from an AML patient (80% blasts; obtained from AllCells LLC) were treated ex vivo with a dose titration of vosaroxin or vehicle for 2 h. Samples were anFull Paper
2015Sanyal, M;Morimoto, M;Baradaran-Heravi, A;Choi, K;Kambham, N;Jensen, K;Dutt, S;Dionis-Petersen, KY;Liu, LX;Felix, K;Mayfield, C;Dekel, B;Bokenkamp, A;Fryssira, H;Guillen-Navarro, E;Lama, G;Brugnara, M;Lücke, T;Olney, AH;Hunley, TE;Polat, AI;Yis, U;Bogdanovic, R;Mitrovic, K;Berry, S;Najera, L;Najafian, B;Gentile, M;Nur Semerci, C;Tsimaratos, M;Lewis, DB;Boerkoel, CF;Lack of IL7Rα expression in T cells is a hallmark of T-cell immunodeficiency in Schimke immuno-osseous dysplasia (SIOD)Clinical immunology (Orlando, Fla.)26499378The protocol and informed consent procedures were provided according to the Declaration of Helsinki guidelines. The clinical data for patients were obtained from questionnaires completed by the attending physician as well as from the medical records.Full Paper
2015Liu, J;Gaj, T;Wallen, MC;Barbas, CF;Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineeringMolecular therapy. Nucleic acids25756962T-activator Dynabeads (Dynal/Life Technologies), and 50 U/ml recombinant interleukin-2 (rIL-2; R&D Systems). Bone marrow-derived CD34+ hematopoietic stem/progenitor cells (HSPCs) were obtained from AllCells, LLC at a purity of >97% (Supplementary FigFull Paper
2015Kim, SY;Theunissen, JW;Balibalos, J;Liao-Chan, S;Babcock, MC;Wong, T;Cairns, B;Gonzalez, D;van der Horst, EH;Perez, M;Levashova, Z;Chinn, L;D'Alessio, JA;Flory, M;Bermudez, A;Jackson, DY;Ha, E;Monteon, J;Bruhns, MF;Chen, G;Migone, TS;A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignanciesBlood cancer journal26024286Additional frozen AML and CLL patient specimens for flow analysis were from AllCells and the University of California San Diego, respectively. Primary solid tumors and normal SaveFull Paper
2015Crews, LA;Jiang, Q;Zipeto, MA;Lazzari, E;Court, AC;Ali, S;Barrett, CL;Frazer, KA;Jamieson, CH;An RNA editing fingerprint of cancer stem cell reprogrammingJournal of translational medicine25889244Peripheral blood or bone marrow samples were processed by Ficoll density centrifugation and viable cells stored in liquid nitrogen. Normal peripheral blood mononuclear cells (MNC) were obtained from AllCells (Alameda, CA). Mononuclear cells from contFull Paper
2015Sloan, DD;Lam, CY;Irrinki, A;Liu, L;Tsai, A;Pace, CS;Kaur, J;Murry, JP;Balakrishnan, M;Moore, PA;Johnson, S;Nordstrom, JL;Cihlar, T;Koenig, S;Targeting HIV Reservoir in Infected CD4 T Cells by Dual-Affinity Re-targeting Molecules (DARTs) that Bind HIV Envelope and Recruit Cytotoxic T CellsPLoS pathogens26539983pended with FACS buffer, and analyzed by flow cytometry using a FACSCalibur (BD Biosciences) and FlowJo software (TreeStar). In Vitro HIV-Infected Cell Killing Assay PBMCs from healthy participants (AllCells) were isolated by Ficoll-plaque gradient.Full Paper
2015Ma, L;Aijima, R;Hoshino, Y;Yamaza, H;Tomoda, E;Tanaka, Y;Sonoda, S;Song, G;Zhao, W;Nonaka, K;Shi, S;Yamaza, T;Transplantation of mesenchymal stem cells ameliorates secondary osteoporosis through interleukin-17-impaired functions of recipient bone marrow mesenchymal stem cells in MRL/lpr miceStem cell research & therapy26012584University (protocol number: A21-044-1). Human MSC isolation and culture Human MSCs were isolated based on the adherent colony-forming unit fibroblasts (CFU-F) method [20]. Bone marrow cells (BMCs) (AllCells, Berkeley, CA, USA) were seeded at 1×107Full Paper
2015Di Lullo, G;Marcatti, M;Heltai, S;Brunetto, E;Tresoldi, C;Bondanza, A;Bonini, C;Ponzoni, M;Tonon, G;Ciceri, F;Bordignon, C;Protti, MP;Th22 cells increase in poor prognosis multiple myeloma and promote tumor cell growth and survivalOncoimmunology26155400had approved the study protocol and written informed consent was obtained from all donors. Blood samples of 15 healthy donors from the Institutional Blood Bank and BM aspirates from 4 healthy donors (AllCells, Life Science Division) were used as contFull Paper
2015Alva, A;Friedlander, T;Clark, M;Huebner, T;Daignault, S;Hussain, M;Lee, C;Hafez, K;Hollenbeck, B;Weizer, A;Premasekharan, G;Tran, T;Fu, C;Ionescu-Zanetti, C;Schwartz, M;Fan, A;Paris, P;Circulating Tumor Cells as Potential Biomarkers in Bladder CancerThe Journal of urology25912492candidacy for cisplatin based chemotherapy and 4) candidacy for radical cystectomy with curative intent. Two patients were lost to followup. Of the patients 16 underwent pathological staging at cystectomy. A second cohort of 11 patients with metastasFull Paper
2015Yang, J;Khan, M;Zhang, L;Ren, X;Guo, J;Feng, Y;Wei, S;Zhang, W;Antimicrobial surfaces grafted random copolymers with REDV peptide beneficial for endothelializationJournal of materials chemistry. B32264578Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells Biomart (Shanghai) and cultured in high glucose DMEM supplemented with 10% FBS in an incubator ( SaveFull Paper
2015Fernandes, S;Chong, JJH;Paige, SL;Iwata, M;Torok-Storb, B;Keller, G;Reinecke, H;Murry, CE;Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac RepairStem cell reports26607951vol/vol), supplemented with L-glutamine, ascorbic acid, transferrin, monothioglycerol, cyclosporine A (200 nM, Wako), and Y-27632 (10 μM). Fresh bone marrow mononuclear cells were purchased from AllCells LLC (ABM024). After overnight shipment, theyFull Paper
2015McIntosh, BE;Brown, ME;Duffin, BM;Maufort, JP;Vereide, DT;Slukvin, II;Thomson, JA;Nonirradiated NOD,B6.SCID Il2rγ-/- Kit(W41/W41) (NBSGW) mice support multilineage engraftment of human hematopoietic cellsStem cell reports25601207cedures for oligos and allelic determination. The University of Wisconsin Medical School’s Animal Care and Use Committee authorized all experiments. Human Cell Engraftment Frozen human CD34+ CBCs (AllCells) were thawed per manufacturer’s recommendatFull Paper
2015Tang, W;Song, H;Cai, W;Shen, X;Real Time Monitoring of Inhibition of Adipogenesis and Angiogenesis by (-)-Epigallocatechin-3-Gallate in 3T3-L1 Adipocytes and Human Umbilical Vein Endothelial CellsNutrients26516907TGTCTTTA-3′ and reverse, 5′-AGCCCTCCACCTCCCTGTAG-3′; β-actin forward, 5′-CCTCTATGCCAACACAGT-3′ and reverse, 5′-AGCCACCAATCCACACAG 3′. 2.8. Co-Culture of HUVEC and 3T3-L1 Cells HUVECs (ALLCELLS, Shanghai, China) were seeded (6000 cells/well) into E-pFull Paper
2015Griffin, DO;Goff, SP;HIV-1 Is Restricted prior to Integration of Viral DNA in Primary Cord-Derived Human CD34+ CellsJournal of virology25995256CD34 cells were isolated with magnetic beads from cord blood samples obtained from the Long Island Blood Service or obtained preisolated from AllCells (11, 12). CD34 cells were put SaveFull Paper
2015Wu, X;Sereno, AJ;Huang, F;Lewis, SM;Lieu, RL;Weldon, C;Torres, C;Fine, C;Batt, MA;Fitchett, JR;Glasebrook, AL;Kuhlman, B;Demarest, SJ;Fab-based bispecific antibody formats with robust biophysical properties and biological activitymAbs25774965Untreated, purified T cells from a human donor were purchased from ALLCELLS (Cat#PB009-1F). The cells were thawed and washed in 10% FBS (heat inactivated, Corning) plus 50 SaveFull Paper
2015Li, X;Tian, F;Wang, F;The effects of black soybean (Glycine max var.) on chronic cervical pain of sedentary office workers in a northern Chinese populationFood & function262954835.0 × 105 cells per ml was placed into each well of a 96-well plate (ALLCELLS, Shanghai, China). After 24 h, cells were incubated with 1 Ci per well of [3H]thymidine (67 Ci mmol−1) for five h, and then collected using a Brandell 96-sample harvester (Full Paper
2015Mata, MF;Lopes, JP;Ishikawa, M;Alaiti, MA;Cabral, JM;da Silva, CL;Costa, MA;Scaling up the ex vivo expansion of human circulating CD34(+)progenitor cells with upregulation of angiogenic and anti-inflammatory potentialCytotherapy26475753CD34 + cells isolated from mobilized peripheral blood of healthy donors (n = 5) were obtained from AllCells and were cultured for 7 days in 30-mL cell culture bags (VueLife FEP Bag, SaveFull Paper
2015Ma, Y;Reilly, S;Fischer, S;Henshaw, M;Pierce, J;Poudel, A;Reems, J;Human platelet lysate produced at industrial scale for use as fetal bovine serum replacement in cell manufacturing protocolsCytotherapyTissue Origin: 9 different donors of fresh human bone marrow (hBM) from the posterior iliac crest were obtained from Lonza or AllCells. 6 different donors of fresh human lipoaspirate SaveFull Paper
2015Fierro, FA;O'Neal, AJ;Beegle, JR;Chávez, MN;Peavy, TR;Isseroff, RR;Egaña, JT;Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cellsFrontiers in cell and developmental biology26579521r bone marrow samples of healthy human donors who gave informed consent to the research protocols (approved by the institutional review board of the Technical University of Dresden) or purchased from Allcells (Alameda, CA). Mononuclear cell (MNC) fraFull Paper
2015Haqqani, AA;Marek, SL;Kumar, J;Davenport, M;Wang, H;Tilton, JC;Central memory CD4+ T cells are preferential targets of double infection by HIV-1Virology journal26559763Normal donor samples were de-identified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. A 10 year-old male with the SaveFull Paper
2015Huang, H;Saravia, J;You, D;Shaw, AJ;Cormier, SA;Impaired gamma delta T cell-derived IL-17A and inflammasome activation during early respiratory syncytial virus infection in infantsImmunology and cell biology25267484tes 2 hr prior to RSV infection. Sample isolation purity and adoptive transfer efficiency were verified by flow cytometry. CBMC/PBMC RIG-I activation Purified CBMCs and PBMCs from individual donors (AllCells.com) were thawed and counted according toFull Paper
2015Lynch, PJ;Thompson, EE;McGinnis, K;Rovira Gonzalez, YI;Lo Surdo, J;Bauer, SR;Hursh, DA;Chromatin Changes at the PPAR-γ2 Promoter During Bone Marrow-Derived Multipotent Stromal Cell Culture Correlate With Loss of Gene Activation PotentialStem cells (Dayton, Ohio)25640287BM-MSCs from six donors were purchased from AllCells (Alameda, CA, http://www.allcells.com/) and Lonza (Allendale, NJ, http://www.lonza.com/) (Table 1). All BM-MSCs used here were previously expanded, characterized, and cryopreserved for experimentatFull Paper
2015Münchow, EA;Albuquerque, MT;Zero, B;Kamocki, K;Piva, E;Gregory, RL;Bottino, MC;Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regenerationDental materials : official publication of the Academy of Dental Materials26116414The guidelines provided by the International Standards Organization/ISO [35] were followed in order to detect toxicity levels of the electrospun membranes [34]. Human dental pulp stem cells (hDPSCs, AllCells LLC., Alameda, CA, USA) obtained from permFull Paper
2015Babad, J;Mukherjee, G;Follenzi, A;Ali, R;Roep, BO;Shultz, LD;Santamaria, P;Yang, OO;Goldstein, H;Greiner, DL;DiLorenzo, TP;Generation of β cell-specific human cytotoxic T cells by lentiviral transduction and their survival in immunodeficient human leucocyte antigen-transgenic miceClinical and experimental immunology25302633Several β cell antigens recognized by T cells in the non-obese diabetic (NOD) mouse model of type 1 diabetes (T1D) are also T cell targets in the human disease. While numerous SaveFull Paper
2015Liu, A;Wang, Y;Ding, Y;Baez, I;Payne, KJ;Borghesi, L;Cutting Edge: Hematopoietic Stem Cell Expansion and Common Lymphoid Progenitor Depletion Require Hematopoietic-Derived, Cell-Autonomous TLR4 in a Model of Chronic EndotoxinJournal of immunology (Baltimore, Md. : 1950)26276875De-identified cord blood cells were obtained with approval from the University of Pittsburgh School of Medicine Institutional Review Board. Purified CD34+ cord blood cells were purchased from AllCells, or cord blood obtained from Loma Linda UniversitFull Paper
2015Katano, I;Takahashi, T;Ito, R;Kamisako, T;Mizusawa, T;Ka, Y;Ogura, T;Suemizu, H;Kawakami, Y;Ito, M;Predominant development of mature and functional human NK cells in a novel human IL-2-producing transgenic NOG mouseJournal of immunology (Baltimore, Md. : 1950)25712215Germany) in combination with biotin-conjugated anti-mouse CD45 mAb (BD Biosciences), all cells, with the exception of human CD56+ NK cells, were magnetically labeled and eliminated using a MACS LD column (Miltenyi Biotec). The purity of the isolatedFull Paper
2015Montezuma-Rusca, JM;Moir, S;Kardava, L;Buckner, CM;Louie, A;Kim, LJ;Santich, BH;Wang, W;Fankuchen, OR;Diaz, G;Daub, JR;Rosenzweig, SD;Chun, TW;Li, Y;Braylan, RC;Calvo, KR;Fauci, AS;Bone marrow plasma cells are a primary source of serum HIV-1-specific antibodies in chronically infected individualsJournal of immunology (Baltimore, Md. : 1950)25681347obtained from posterior iliac crests under conscious sedation and local anesthesia. In addition, cryopreserved bone marrow mononuclear cells (BMMCs) were obtained from commercially available sources (Allcells or Stemcell Technologies)Full Paper
2015Kang, HS;Choi, SH;Kim, BS;Choi, JY;Park, GB;Kwon, TG;Chun, SY;Advanced Properties of Urine Derived Stem Cells Compared to Adipose Tissue Derived Stem Cells in Terms of Cell Proliferation, Immune Modulation and Multi DifferentiationJournal of Korean medical science26713051or separated-(trans-well chamber, Corning, Corning, NY, USA) cultured with both stem cells; each well was composed with 1:100, 1:50, 1:25, or 1:12.5 stem cell to PBMC ratio. PBMCs were acquired from AllCells (Emeryville, CA, USA) and activated with 5Full Paper
2015Wu, W;Zhang, X;Lv, H;Liao, Y;Zhang, W;Cheng, H;Deng, Z;Shen, J;Yuan, Q;Zhang, Y;Shen, W;Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesionInternational journal of molecular medicine26260137cells (HUVECs) were purchased from AllCells (Shanghai, China) and cultured in 0.5% gelatin-coated dishes in endothelial cell growth medium (EGM; AllCells) according to the SaveFull Paper
2015Kamocki, K;Nör, JE;Bottino, MC;Dental pulp stem cell responses to novel antibiotic-containing scaffolds for regenerative endodonticsInternational endodontic journal25425048fetal bovine serum (FBS, Hyclone, Logan, UT, USA) and 1% penicillin- streptomycin (Sigma-Aldrich) in a humidified incubator at 37°C, with 5% CO2, was used to culture hDPSC (catalogue number - DP003F, AllCells LLC, Alameda, CA, USA) obtained from permFull Paper
2015Press, T;Viale-Bouroncle, S;Felthaus, O;Gosau, M;Morsczeck, C;EGR1 supports the osteogenic differentiation of dental stem cellsInternational endodontic journal24749562Dental follicle cells (DFCs), also called dental follicle stem cells (DFSCs), were purchased from AllCells© (Alameda, CA, USA). All cells were grown in standard basal medium, Dulbecco′s modified Eagle′s medium (DMEM; Sigma-Aldrich, Munich, Germany) sFull Paper
2015Lu, Q;Pandya, M;Rufaihah, AJ;Rosa, V;Tong, HJ;Seliktar, D;Toh, WS;Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel SystemStem cells international26124841used in this study were obtained commercially from AllCells, LLC. DPSCs were cultured in Dulbecco's modified eagle's medium (DMEM) (low glucose, Biowest, France) supplemented SaveFull Paper
2015Chen, Q;Ye, W;Jian Tan, W;Mei Yong, KS;Liu, M;Qi Tan, S;Loh, E;Te Chang, K;Chye Tan, T;Preiser, PR;Chen, J;Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in HumanScientific reports26456148ercoll. Contaminating red blood cells were lysed with ACK lysis buffer (Life Technologies). Isolation of CD56+ cells from MNCs was performed via cell sorting. Human adult BM MNCs were purchased from AllCells (Alameda, CA). To isolate MNCs from fetalFull Paper
2015Su, M;Hu, R;Jin, J;Yan, Y;Song, Y;Sullivan, R;Lai, L;Efficient in vitro generation of functional thymic epithelial progenitors from human embryonic stem cellsScientific reports26044259ed (2 Gy), transplanted under the kidney capsule with the solidified reaggregate of the hESC-derived cells, and injected i.v. with human bone marrow CD34+ cells (1 × 105 cells/mouse, obtained from AllCells, LLC, Alameda, CA). Twelve to 24 weeks afterFull Paper
2015Nixon, CC;Schwartz, BH;Dixit, D;Zack, JA;Vatakis, DN;Cocaine exposure impairs multilineage hematopoiesis of human hematopoietic progenitor cells mediated by the sigma-1 receptor [corrected]Scientific reports25728014Mononuclear cells were obtained from the buffy coats of either processed fetal liver or cord blood or adult bone marrow (Allcells, Alameda, CA) following Ficoll-Hypaqe density gradient SaveFull Paper
2015Feitosa, SA;Münchow, EA;Al-Zain, AO;Kamocki, K;Platt, JA;Bottino, MC;Synthesis and characterization of novel halloysite-incorporated adhesive resinsJournal of dentistry26334950Next, human dental pulp stem cells (CD73, CD90, CD105, and CD166 positive) obtained from permanent third molars (hDPSCs, catalog number—DP003F, AllCells LLC, Alameda, CA, USA) [32] were cultured in DMEM supplemented with 10% FBS were seeded at the ceFull Paper
2015Lei, L;Wang, S;Wu, H;Ju, W;Peng, J;Qahtan, AS;Chen, C;Lu, Y;Peng, J;Zhang, X;Nie, H;Optimization of release pattern of FGF-2 and BMP-2 for osteogenic differentiation of low-population density hMSCsJournal of biomedical materials research. Part A24639043Fresh whole bone marrow was acquired from AllCells (Emeryville, CA). 0.5 mL of RosetteSep The hMSCs were isolated according to AllCells' protocol. Cells from passages 4-9 were SaveFull Paper
2015Poleganov, MA;Eminli, S;Beissert, T;Herz, S;Moon, JI;Goldmann, J;Beyer, A;Heck, R;Burkhart, I;Barea Roldan, D;Türeci, Ö;Yi, K;Hamilton, B;Sahin, U;Efficient Reprogramming of Human Fibroblasts and Blood-Derived Endothelial Progenitor Cells Using Nonmodified RNA for Reprogramming and Immune EvasionHuman gene therapy26381596Cord blood EPCs were derived from 5E7 frozen cord blood MNCs (AllCells). Both peripheral and cord blood MNCs were washed and cultured on rat collagen type I (BD Bioscience)- SaveFull Paper
2015Suenaga, H;Furukawa, KS;Suzuki, Y;Takato, T;Ushida, T;Bone regeneration in calvarial defects in a rat model by implantation of human bone marrow-derived mesenchymal stromal cell spheroidsJournal of materials science. Materials in medicine26449444p). After a recovery period of 8 weeks, bone regeneration at the defect sites was evaluated Isolation and culturing of human MSCs Fresh BM samples of 3-4 anonymous adult donors were obtained from AllCells (Berkeley, CA), and MSCs were isolated, as dFull Paper
2015Uchiyama, T;Kawabata, H;Miura, Y;Yoshioka, S;Iwasa, M;Yao, H;Sakamoto, S;Fujimoto, M;Haga, H;Kadowaki, N;Maekawa, T;Takaori-Kondo, A;The role of growth differentiation factor 15 in the pathogenesis of primary myelofibrosisCancer medicine26276681Normal human BM mononuclear cells were separated from BM aspirates (AllCells) using Ficoll-Hypaque-Plus solution (GE Healthcare, Tokyo, Japan). CD34 + HPCs (Hematopoietic SaveFull Paper
2015Wu, H;Fan, F;Liu, Z;Zhang, F;Liu, Y;Wei, Z;Shen, C;Cao, Y;Wang, A;Lu, Y;The angiogenic responses induced by release of angiogenic proteins from tumor cell-activated platelets are regulated by distinct molecular pathwaysIUBMB life26283102Gibco, Grand Island, NY), 100 U/mL penicillin and 100 μg/mL streptomycin, and grown in a 95% air and 5% CO2 humidified atmosphere at 37°C in Primary HUVECs were purchased from Allcells (Shanghai, China) and cultured in EBM media (Allcells) accordingFull Paper
2015Bam, R;Khan, S;Ling, W;Randal, SS;Li, X;Barlogie, B;Edmondson, R;Yaccoby, S;Primary myeloma interaction and growth in coculture with healthy donor hematopoietic bone marrowBMC cancer26545722itute. NBM coculture system Preparation of the NBM system is illustrated in Fig. 1a. Freshly obtained (via overnight shipping) whole BM cells from healthy adult (>40 years old) donors (n = 20, Allcells, Alameda, CA) were lysed with RBC lysis bufferFull Paper
2015Liao, W;Jordaan, G;Nham, P;Phan, RT;Pelegrini, M;Sharma, S;Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimensBMC cancer26474785In all the CLL specimens, more than 90 % of isolated cells were CD19+ by flow cytometry analysis. Total RNA from isolated B cells (five different normal donors, caucasian males) was purchased from ALLCELLS (Alameda, CA). IGVH mutation (ImmunoglobulinFull Paper
2015Liu, L;Yu, H;Huang, X;Tan, H;Li, S;Luo, Y;Zhang, L;Jiang, S;Jia, H;Xiong, Y;Zhang, R;Huang, Y;Chu, CC;Tian, W;A novel engineered VEGF blocker with an excellent pharmacokinetic profile and robust anti-tumor activityBMC cancer25881012) in complete HUVEC-adapted medium (Cat#H-004, ALLCELLS) were incubated in 6 cm dishes at 37C, 5% CO 2 for 24 hours, cells were starved for 2 hours and then challenged for 15 SaveFull Paper
2015Priestman, MA;Wang, Q;Jernigan, FE;Chowdhury, R;Schmidt, M;Lawrence, DS;Multicolor monitoring of the proteasome's catalytic signatureACS chemical biology25347733m. All Proteasome inhibitors were purchased from Enzo Life Sciences. HeLa cells were purchased from the tissue culture facility at the University of North Carolina. Normal B-cells were purchased from AllCells. OCI-Ly3 and HBL-1 cells were provided byFull Paper
2015Yi, Y;Qi, H;Yuan, J;Wang, R;Weng, S;He, J;Dong, C;Functional characterization of viral tumor necrosis factor receptors encoded by cyprinid herpesvirus 3 (CyHV3) genomeFish & shellfish immunology26052019Primary human umbilical vein endothelial cells (HUVECs) were cultured in appropriate media according to the manufacturer's protocol (AllCells, China). The full length of CyHV3 ORF4 SaveFull Paper
2015Doss, JF;Corcoran, DL;Jima, DD;Telen, MJ;Dave, SS;Chi, JT;A comprehensive joint analysis of the long and short RNA transcriptomes of human erythrocytesBMC genomics26573221les as the input list to test for the enrichment of top RBC expressing transcripts against those more specific to D8 samples or the PBMC samples. Cell culture Mobilized Peripheral Blood CD34+ Cells (AllCells) were grown in StemLine II media containiFull Paper
2015Shi, J;Zhou, J;Zhang, M;Microcystins induces vascular inflammation in human umbilical vein endothelial cells via activation of NF-BMediators of InflammationPrimary HUVECs were obtained from Allcells (Shanghai, China) and cells in passages 3-5 were used in this study. Cells were grown in an RPMI 1640 medium containing 20% fetal SaveFull Paper
2015Zhang, Z;Luo, X;Xu, H;Wang, L;Jin, X;Chen, R;Ren, X;Lu, Y;Fu, M;Huang, Y;He, J;Fan, Z;Bone marrow stromal cell-derived extracellular matrix promotes osteogenesis of adipose-derived stem cellsCell biology international25264269BM-MSCs were purchased from ALLCELLS (Emeryville, CA) and expanded in culture medium [α-Minimal Essential Medium (Life Technologies, Grand Island, NY) supplemented with SaveFull Paper
2015Zhang, Q;Wang, LQ;Wong, KY;Li, ZY;Chim, CS;Infrequent DNA methylation of miR-9-1 and miR-9-3 in multiple myelomaJournal of clinical pathology25855800peripheral blood (PB) and three BM normal buffy coat), one CD138-sorted healthy marrow plasma cells (AllCells, California, USA), 10 cell lines (KMS-12-PE, LP-1, MOLP-8, NCI-H929, OPM-2, RPMI-8226, U-266, WL-2, OCI-MY5 and JJN-3), and primary MM marroFull Paper
2015Wang, DY;Gopinath, S;Lagacé, RE;Norona, W;Hennessy, LK;Short, ML;Mulero, JJ;Developmental validation of the GlobalFiler(®) Express PCR Amplification Kit: A 6-dye multiplex assay for the direct amplification of reference samplesForensic science international. Genetics26226223To demonstrate the sensitivity of the GlobalFiler Express Kit, white blood cells (WBCs) from two individuals were purchased from AllCells, LLC (Emeryville, CA) and quantitated using SaveFull Paper
2015Mahajan, S;Hogan, JK;Shlyakhter, D;Oh, L;Salituro, FG;Farmer, L;Hoock, TC;VX-509 (decernotinib) is a potent and selective janus kinase 3 inhibitor that attenuates inflammation in animal models of autoimmune diseaseThe Journal of pharmacology and experimental therapeutics25762693Sigma-Aldrich (St. Louis, MO); human B cells from AllCells (Emeryville, CA); Roswell Park Memorial Institute medium tissue culture medium, phosphate-buffered saline (PBS), and 10,000 U/ml penicillin-streptomycin from Life Technologies (Grand Island,Full Paper
2015Shabbir, A;Cox, A;Rodriguez-Menocal, L;Salgado, M;Van Badiavas, E;Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In VitroStem cells and development25867197Human MSCs were isolated and expanded from normal donor bone marrow acquired from AllCells LLC (Emeryville, CA, www.allcells.com). Three different donors were used for these experiments. MSCs were isolated by the plastic adherence method as describedFull Paper
2015Limb, JK;Song, D;Jeon, M;Han, SY;Han, G;Jhon, GJ;Bae, YS;Kim, J;2-(trimethylammonium)ethyl (R)-3-methoxy-3-oxo-2-stearamidopropyl phosphate promotes megakaryocytic differentiation of myeloid leukaemia cells and primary human CD34⁺ haematopoietic stem cellsJournal of tissue engineering and regenerative medicine23166016Human bone marrow-derived CD34+ cells were obtained from AllCells (Emeryville, CA, USA). Cells were plated at 8 × 104 cells/well in a 12-well plate and cultured for 16 days in Iscove's modified Dulbecco's medium (IMDM; Invitrogen) with 15% BIT 9500 (Full Paper
2015Leelakanok, N;Fischer, CL;Bates, AM;Guthmiller, JM;Johnson, GK;Salem, AK;Brogden, KA;Brogden, NK;Cytotoxicity of HBD3 for dendritic cells, normal human epidermal keratinocytes, hTERT keratinocytes, and primary oral gingival epithelial keratinocytes in cell culture conditionsToxicology letters26367466Human β-defensin 3 (HBD3) is a prominent host defense peptide. In our recent work, we observed that HBD3 modulates pro-inflammatory agonist-induced chemokine and cytokine SaveFull Paper
2015Gupta, MS;Nicoll, SB;Duration of TGF-β3 Exposure Impacts the Chondrogenic Maturation of Human MSCs in Photocrosslinked Carboxymethylcellulose HydrogelsAnnals of biomedical engineering25384834Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density centrifugation as previously described.15,28 These primary cells were then cultured using MSC growth medium containing Low Glucose Dulbecco’s Modified Eagle MFull Paper
2015Marino, MP;Panigaj, M;Ou, W;Manirarora, J;Wei, CH;Reiser, J;A scalable method to concentrate lentiviral vectors pseudotyped with measles virus glycoproteinsGene therapy25608718Human peripheral blood mononuclear cells were obtained from AllCells, LLC (Alameda, CA, USA). T cells were enriched by negative selection using the EasySep Human T Cell Enrichment Cocktail and EasySep D Magnetic Particles (Stem Cell Technologies, VanFull Paper
2015Shahaduzzaman, MD;Mehta, V;Golden, JE;Rowe, DD;Green, S;Tadinada, R;Foran, EA;Sanberg, PR;Pennypacker, KR;Willing, AE;Human umbilical cord blood cells induce neuroprotective change in gene expression profile in neurons after ischemia through activation of Akt pathwayCell transplantation25413246HUCB Cell Preparation Just prior to the OGD experiment, cryopreserved HUCB mononuclear cells (AllCells, LLC, Alameda, CA, USA) were thawed in a 37ºC water bath and then washed twice in 10 ml PBS (136.9 mM NaCl, 2.7 mM KCl, 10.1 mM Na2HPO4, 1.8 mM KH2Full Paper
2015Fukui, T;Mifune, Y;Matsumoto, T;Shoji, T;Kawakami, Y;Kawamoto, A;Ii, M;Akimaru, H;Kuroda, T;Horii, M;Yokoyama, A;Alev, C;Kuroda, R;Kurosaka, M;Asahara, T;Superior Potential of CD34-Positive Cells Compared to Total Mononuclear Cells for Healing of Nonunion Following Bone FractureCell transplantation24800622We purchased human GM-PB CD34+ cells obtained from three healthy males (20-year-old African-American; 19-year-old Caucasian; 21-year-old Caucasian) and human GM-PB MNCs obtained from three healthy males (25-year-old African-American; 25-year-old AfriFull Paper
2015Wada, A;Ito, A;Iitsuka, H;Tsuneyama, K;Miyazono, T;Murakami, J;Shibahara, N;Sakurai, H;Saiki, I;Nakayama, T;Yoshie, O;Koizumi, K;Sugiyama, T;Role of chemokine CX3CL1 in progression of multiple myeloma via CX3CR1 in bone microenvironmentsOncology reports25962684GT-3′ and antisense, 5′-CAT GTG GGC CAT GAG GTC CAC CAC-3′. The primers for CX3CR1 were as follows: sense, 5′-TGG CCT TGT CTG ATC TGC TGT TTG-3′ and antisense, 5′-ATG GCT TTG GCT TTC TTG TGG TTC-3′. The RNA from healthy donor plasma cell was purchaseFull Paper
2015Huang, Q;Zhou, C;Chen, X;Dong, B;Chen, S;Zhang, N;Liu, Y;Li, A;Yao, M;Miao, J;Li, Q;Wang, Z;Prodrug AST-003 Improves the Therapeutic Index of the Multi-Targeted Tyrosine Kinase Inhibitor SunitinibPloS one26513662Dr. Jiang Li at the Sun Yat-Sen University School of Medicine, which were purchased from Type Culture Collection of the Chinese Academy of Sciences in Shanghai, China. HUVEC cells were purchased from Allcells, Inc. The cell lines A549, Caki-1, HT-29,Full Paper
2015Lanman, RB;Mortimer, SA;Zill, OA;Sebisanovic, D;Lopez, R;Blau, S;Collisson, EA;Divers, SG;Hoon, DS;Kopetz, ES;Lee, J;Nikolinakos, PG;Baca, AM;Kermani, BG;Eltoukhy, H;Talasaz, A;Analytical and Clinical Validation of a Digital Sequencing Panel for Quantitative, Highly Accurate Evaluation of Cell-Free Circulating Tumor DNAPloS one26474073To assess the accuracy of our test, two micrograms of genomic DNA from 20 healthy and young donors (AllCells, Alameda, CA) were sent to an outside reference lab (Ambry Genetics, SaveFull Paper
2015Breese, EH;Buechele, C;Dawson, C;Cleary, ML;Porteus, MH;Use of Genome Engineering to Create Patient Specific MLL Translocations in Primary Human Hematopoietic Stem and Progenitor CellsPloS one26351841When fresh umbilical cord blood was not available, commercially available frozen human CD34+ cells isolated from umbilical cord blood were used (AllCells LLC or StemCell SaveFull Paper
2015Bose, C;Megyesi, JK;Shah, SV;Hiatt, KM;Hall, KA;Karaduta, O;Swaminathan, S;Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic FibrosisPloS one26305890ked immunosorbent assay (ELISA) by a commercially available kit (GenWay Biotech Inc., San Diego, CA) as instructed by the manufacturer. Cell culture and treatments Commercially available human PBMC (AllCells, Emeryville, CA) were used for in vitro sFull Paper
2015Ge, HY;Han, ZJ;Tian, P;Sun, WJ;Xue, DX;Bi, Y;Yang, ZH;Liu, P;VEGFA Expression Is Inhibited by Arsenic Trioxide in HUVECs through the Upregulation of Ets-2 and miRNA-126PloS one26274316, Shanghai, China) were cultured in complete medium (HUVEC-004, supplied by AllCells) in an atmosphere containing 5% CO 2 at 37C. All experiments were performed according to SaveFull Paper
2015Ninomiya, S;Kawano, M;Abe, T;Ishikawa, T;Takahashi, M;Tamura, M;Takahashi, Y;Nashimoto, M;Potential small guide RNAs for tRNase ZL from human plasma, peripheral blood mononuclear cells, and cultured cell linesPloS one25730316myeloma patients (MM1-MM3) were also obtained from AllCells. Written informed consent was obtained from each person by AllCells. In the case that human samples to be analyzed SaveFull Paper
2015Liu, Z;Benard, O;Syeda, MM;Schuster, VL;Chi, Y;Inhibition of Prostaglandin Transporter (PGT) Promotes Perfusion and Vascularization and Accelerates Wound Healing in Non-Diabetic and Diabetic RatsPloS one26230411lonal antibody (LifeSpan BioSciences, Seattle, WA, USA) interacting with rat tissues was used and the dilution was 1: 250. Cell Culture Fresh primary human bone marrow CD34+ cells were obtained from AllCells (Alameda, CA, USA) and cultured accordingFull Paper
2015Placencio, VR;Ichimura, A;Miyata, T;DeClerck, YA;Small Molecule Inhibitors of Plasminogen Activator Inhibitor-1 Elicit Anti-Tumorigenic and Anti-Angiogenic ActivityPloS one26207899HUVEC (human umbilical vein endothelial cells) were purchased from AllCells and maintained as recommended. HT1080 and HCT116 cells were cultured in DMEM medium SaveFull Paper
2015Fujii, H;Luo, ZJ;Kim, HJ;Newbigging, S;Gassas, A;Keating, A;Egeler, RM;Humanized Chronic Graft-versus-Host Disease in NOD-SCID il2rγ-/- (NSG) Mice with G-CSF-Mobilized Peripheral Blood Mononuclear Cells following Cyclophosphamide and Total Body IrradiationPloS one26176698G-CSF-mobilized human peripheral blood mononuclear cells (G-hPBMCs; 3 donors) and CD34 + cells from G-CSF-mobilized human PB were purchased from ALLCELLS (Emeryvill, CA SaveFull Paper
2015Yan, M;Wen, J;Liang, M;Lu, Y;Kamata, M;Chen, IS;Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAsPloS one26035832edium (Life Technologies, Carlsbad, CA) containing 10% FBS and supplemented with 2mM glutamine, 100 U/ml of penicillin, and 100 μg/ml of streptomycin. Mobilized human CD34+ hematopoietic stem cells (AllCells, Alameda, CA) were cultured in StepSpan SFFull Paper
2015Patterson, SD;Rossi, JM;Paweletz, KL;Fitzpatrick, VD;Begley, CG;Busse, L;Elliott, S;McCaffery, I;Functional EpoR pathway utilization is not detected in primary tumor cells isolated from human breast, non-small cell lung, colorectal, and ovarian tumor tissuesPloS one25807104were adjusted to 106 viable cells/mL prior to growth factor stimulation. For additional cell culture conditions, see S1 Appendix. Erythroid Progenitor-Cell (EPC) Assay Human CD34+ progenitor cells (AllCells Inc., Emeryville, CA) were isolated from bFull Paper
2015Yu, L;Croze, E;Yamaguchi, KD;Tran, T;Reder, AT;Litvak, V;Volkert, MR;Induction of a unique isoform of the NCOA7 oxidation resistance gene by interferon β-1bJournal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research25330068Peripheral blood mononuclear cells (PBMCs) derived from healthy individuals were obtained from AllCells. PBMCs (1×106cells/mL) and cultured cells were incubated at 37°C and 5.0% CO2 and grown in Eagle's MEM supplemented with 10% v/v heat-inactivatedFull Paper
2015Liang, X;Potter, J;Kumar, S;Zou, Y;Quintanilla, R;Sridharan, M;Carte, J;Chen, W;Roark, N;Ranganathan, S;Ravinder, N;Chesnut, JD;Rapid and highly efficient mammalian cell engineering via Cas9 protein transfectionJournal of biotechnology26003884CD34+ cord blood cells were purchased from AllCells. A549 cells, U-2 OS cells, Neuro-2a (N2A) cells were purchased from ATCC. MEF feeder cells and ROCK inhibitor Y-27632 were SaveFull Paper
2015Kempema, AM;Widen, JC;Hexum, JK;Andrews, TE;Wang, D;Rathe, SK;Meece, FA;Noble, KE;Sachs, Z;Largaespada, DA;Harki, DA;Synthesis and antileukemic activities of C1-C10-modified parthenolide analoguesBioorganic & medicinal chemistry26088334Frozen human mononuclear bone marrow cells were purchased from AllCells (Cat. #ABM011F). These bone marrow cells were from two donors (#5630 [Lot #BM4565] and #4887 [Lot # SaveFull Paper
2015Saraf, S;Araki, H;Petro, B;Park, Y;Taioli, S;Yoshinaga, KG;Koca, E;Rondelli, D;Mahmud, N;Ex vivo expansion of human mobilized peripheral blood stem cells using epigenetic modifiersTransfusion25363624Growth factor-mobilized human MPB or BM cells were obtained from healthy donors either from a commercially available source (AllCells, LLC, Emeryville, CA) or from aliquots of SaveFull Paper
2015Geiger, A;Walker, A;Nissen, E;Human fibrocyte-derived exosomes accelerate wound healing in genetically diabetic miceBiochemical and biophysical research communications26454169Human donor fibrocytes were obtained from leukapheresis packs (AllCells), as previously reported for experiments requiring a high yield of these cells [5], [16], [28]. Viable CD45+[CD3/CD19/CD20/CRTH2]−CD16−CD115−CD11b+CD34+ fibrocytes were enumerateFull Paper
2015Takahashi, T;Katano, I;Ito, R;Ito, M;Visualization of the human CD4⁺ T-cell response in humanized HLA-DR4-expressing NOD/Shi-scid/γc(null) (NOG) mice by retrogenic expression of the human TCR geneBiochemical and biophysical research communications254625652.2. CD34+ hematopoietic stem cells Umbilical CD34+ cells were obtained from AllCells (Alameda, CA). 2.3. DNA constructs BLG-specific CD4+ T-cell clones (HA5.7 and YA4) were kindly provided by Dr. Kondo [14]. The TCRα and β genes were isolated and tFull Paper
2015Antebi, B;Zhang, Z;Wang, Y;Lu, Z;Chen, XD;Ling, J;Stromal-cell-derived extracellular matrix promotes the proliferation and retains the osteogenic differentiation capacity of mesenchymal stem cells on three-dimensional scaffoldsTissue engineering. Part C, Methods24965227Primary bone-marrow-derived mononuclear cells (MNCs) from human donors 20-30 years of age were purchased from AllCells, LLC (Emeryville, CA). MNCs were cultured on standard SaveFull Paper
2015Akiyama, H;Kobayashi, A;Ichimura, M;Tone, H;Nakatani, M;Inoue, M;Tojo, A;Kagami, H;Comparison of manual and automated cultures of bone marrow stromal cells for bone tissue engineeringJournal of bioscience and bioengineering25907575Fresh human bone marrows, obtained from three healthy donors (25- and 28-year-old males and a 38-year-old female), were purchased from AllCells (Emeryville, CA, USA). Each bone marrow was divided into two 20-mL groups, and the manual and automated prFull Paper
2015Natu, VP;Dubey, N;Loke, GC;Tan, TS;Ng, WH;Yong, CW;Cao, T;Rosa, V;Bioactivity, physical and chemical properties of MTA mixed with propylene glycolJournal of applied oral science : revista FOB26398513paration of MTA conditioned media The use of human dental pulp stem cells in this study was approved by the Institutional Review Board/NUS (Approval Number: NUS 2094). DPSCs between passages 3 and 6 (Allcells, Alameda, California, USA) were used to eFull Paper
2015Musa, M;Mohd Ali, K;Kannan, TP;Azlina, A;Omar, NS;Chatterji, A;Mokhtar, KI;Effects of Perivitelline Fluid Obtained from Horseshoe Crab on The Proliferation and Genotoxicity of Dental Pulp Stem CellsCell journal26199904xicity was evaluated based on the chromosome aberration (CA) and Ames tests. Materials and Methods Study design This was an in vitro experimental study carried out on DPSCs. Cell culture DPSCs from AllCells (USA, cat no. DP004F) were cultured in meFull Paper
2015Elorza, MT;Human cytomegalovirus genome replication and maintenance during latent infectionThesisHuman CD14 (+) cells purchased (Lonza and ReachBio) or isolated from cord blood were maintained in Iscove DMEM (Hyclone) supplemented with 20% heatinactivated FBS (Atlanta Biologicals), 50 ng/mL M-CSF, 50 ng/mL stem cell factor (SCF), 50 ng/mL G-CSF,Full Paper
2015Kamocki, K;Nör, JE;Bottino, MC;Effects of ciprofloxacin-containing antimicrobial scaffolds on dental pulp stem cell viability-In vitro studiesArchives of oral biology26042622% penicillin-streptomycin (Sigma-Aldrich) placed in a humidified incubator at 37°C, with 5% CO2, was used to culture human dental pulp stem cells (hDPSC, AllCells LLC, Alameda, CA, USA) obtained from permanent third molars. 17,18 The medium was changFull Paper
2015Guo, J;Wang, Q;Wai, D;Zhang, QZ;Shi, SH;Le, AD;Shi, ST;Yen, SL;Visible red and infrared light alters gene expression in human marrow stromal fibroblast cellsOrthodontics & craniofacial research25865533Commercially available human bone marrow aspirates from two healthy adult donors were purchased from AllCells (Emeryville, CA). Human bone marrow stem cells (HBMSCs) were isolated from the aspirates following published protocols10, 11 and then culturFull Paper
2015Thys, RG;Lehman, CE;Pierce, LC;Wang, YH;Environmental and chemotherapeutic agents induce breakage at genes involved in leukemia-causing gene rearrangements in human hematopoietic stem/progenitor cellsMutation research26163765ot been previously connected to leukemogenesis. 2. Materials and methods 2.1. Cells and culture conditions Experiments were performed using primary human CD34+ bone marrow stem and progenitor cells (ALLCELLS), grown in Hematopoietic Growth Medium (LFull Paper
2015Low, A;Mohd Yusof, H;Reza, F;Abdullah Nurul, A;Sritharan, S;Haania Zain Ali, N;Subhi Azeez, H;Husein, A;Gypsum-based biomaterials: Evaluation of physical and mechanical properties, cellular effects and its potential as a pulp linerDental materials journal26235719SHED (AllCells, Chicago, IL, USA) were cultivated under sterile conditions in culture flasks containing growth media in a controlled condition at 37°C under a 5% CO2 humidified atmosphere. The culture media consisted of 10% heat-inactivated fetal bovFull Paper
2015Zhan, H;Ciano, K;Dong, K;Zucker, S;Targeting glutamine metabolism in myeloproliferative neoplasmsBlood cells, molecules & diseases26227854Peripheral blood CD34 + cells from healthy controls were purchased from AllCells . Peripheral blood CD34 + cells from healthy controls were purchased from AllCells . SaveFull Paper
2015Ciaffoni, F;Cassella, E;Varricchio, L;Massa, M;Barosi, G;Migliaccio, AR;Activation of non-canonical TGF-β1 signaling indicates an autoimmune mechanism for bone marrow fibrosis in primary myelofibrosisBlood cells, molecules & diseases25703685Signalling profiling was performed on mononuclear cells obtained from BM (n=3) and spleen (n=6) of patients, normal BM (n=3) (ABM008F-BM3366, ABM008F-BM3527 and ABM008F-BM3600, ALLCELLS, Emeryville, CA) and spleen (n=3) from males (30-years old) whoFull Paper
2015Nazarian, AA;Archibeque, IL;Nguyen, YH;Wang, P;Sinclair, AM;Powers, DA;Characterization of bispecific T-cell Engager (BiTE) antibodies with a high-capacity T-cell dependent cellular cytotoxicity (TDCC) assayJournal of biomolecular screening25477202Normal peripheral blood (NPB) was collected via leukapheresis from normal healthy human donors who were nonsmokers and were not taking any allergy medication (AllCells, SaveFull Paper
2015Suknuntha, K;Ishii, Y;Tao, L;Hu, K;McIntosh, BE;Yang, D;Swanson, S;Stewart, R;Wang, JYJ;Thomson, J;Slukvin, I;Discovery of survival factor for primitive chronic myeloid leukemia cells using induced pluripotent stem cellsStem cell research26561938Bone marrow mononuclear cells from CML patients in the chronic phase were purchased commercially (AllCells or Applied StemCells), or obtained from the patients at the University of Save;w specimens from CML patients in the chronic phase, and controFull Paper
2015Kanjevac, T;Milovanovic, M;Milosevic-Djordjevic, O;Tesic, Z;Ivanovic, M;Lukic, A;Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrationsArchives of Biological SciencesHuman SHED purchased from AllCells, LLC (Emeryville, California, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS, 100 I.U./mL penicillin G and 100 μg/mL streptomycin (Sigma-AldrichFull Paper
2015Gonçalves Silva, I;Gibbs, BF;Bardelli, M;Varani, L;Sumbayev, VV;Differential expression and biochemical activity of the immune receptor Tim-3 in healthy and malignant human myeloid cellsOncotarget26413815e highest grade of purity and available commercially unless otherwise stated. Primary human AML cells Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordaFull Paper
2015Gibbs, BF;Gonçalves Silva, I;Prokhorov, A;Abooali, M;Yasinska, IM;Casely-Hayford, MA;Berger, SM;Fasler-Kan, E;Sumbayev, VV;Caffeine affects the biological responses of human hematopoietic cells of myeloid lineage via downregulation of the mTOR pathway and xanthine oxidase activityOncotarget26384306For this purpose we used primary acute myeloid leukaemia cells AML-PB001F purchased from AllCells (Alameda, CA, USA). Cells were exposed to 0.1 μg/ml SCF (these cells express SaveFull Paper
2015Wang, Y;Li, JX;Wang, YQ;Miao, ZH;Tanshinone I inhibits tumor angiogenesis by reducing Stat3 phosphorylation at Tyr705 and hypoxia-induced HIF-1α accumulation in both endothelial and tumor cellsOncotarget26202747ere obtained from the French National Institute of Health and Medical Research (INSERM, FR), and cultured with complete MCDB131 medium (Invitrogen, Carlsbad, CA, USA). HUVEC cells were purchased from AllCells (Shanghai, China). Human breast cancer MCFull Paper
2015Liu, K;Liu, X;Peng, Z;Sun, H;Zhang, M;Zhang, J;Liu, S;Hao, L;Lu, G;Zheng, K;Gong, X;Wu, D;Wang, F;Shen, L;Retargeted human avidin-CAR T cells for adoptive immunotherapy of EGFRvIII expressing gliomas and their evaluation via optical imagingOncotarget26124178To isolate peripheral blood mononuclear Cells (PBMC), the red blood cells and platelets were removed with PBMC isolation kits according to the manufacturer's instruction (AllCells, SaveFull Paper
2015Ao, JY;Chai, ZT;Zhang, YY;Zhu, XD;Kong, LQ;Zhang, N;Ye, BG;Cai, H;Gao, DM;Sun, HC;Robo1 promotes angiogenesis in hepatocellular carcinoma through the Rho family of guanosine triphosphatases' signaling pathwayTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine26022159Robo1 is a member of the Robo immunoglobulin superfamily of proteins, and it plays an important role in angiogenesis and cancer. In this study, we investigate the role of roundabout 1 SaveFull Paper
2015Patel, P;Mahmud, D;Park, Y;Yoshinaga, K;Mahmud, N;Rondelli, D;Clinical grade isolation of regulatory T cells from G-CSF mobilized peripheral blood improves with initial depletion of monocytesAmerican journal of blood research27069755Tregs were purified from G-PBSC (AllCells) obtained from healthy donors. In some cases CD34 + cells were initially isolated on a ClinMACS device (Miltenyi) by positive selection using SaveFull Paper
2015Woodell-May, JE;Tan, ML;King, WJ;Swift, MJ;Welch, ZR;Murphy, MP;McKale, JM;Characterization of the Cellular Output of a Point-of-Care Device and the Implications for Addressing Critical Limb IschemiaBioResearch open access26634187s collected from the iliac crest from healthy financially compensated subjects who qualified for blood donation as per AABB standards. Aspiration was performed by Poietics-Cambrex (Gaithersburg, MD), AllCells (Alameda, CA), and HemaCare (Van Nuys, CAFull Paper
2015Yao, J;Song, A;Ruan, Z;Zhou, L;Liu, P;Zhu, H;Gong, H;Dong, S;Xu, Y;Jiang, E;Pang, A;Feng, S;Han, M;[Vascular endothelial injury induced by anti-endothelial cell antibody in allogeneic hematopoietic stem cell transplantation]Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi26134010FBS)及BCA定量试剂盒购自美国Thermo Scientific公司;辣 过氧化酶 记的羊抗人IgG购自 美国KPL公司;四甲基联苯胺(TMB)购自美国Tiangen公司;HUVEC及EC完全培养基购自美国Allcells SaveFull Paper
2015Du, C;Weng, X;Hu, W;Lv, Z;Xiao, H;Ding, C;Gyabaah, OA;Xie, H;Zhou, L;Wu, J;Zheng, S;Hypoxia-inducible MiR-182 promotes angiogenesis by targeting RASA1 in hepatocellular carcinomaJournal of experimental & clinical cancer research : CR26126858The HUVECs were obtained from Allcells (Shanghai, China allcells.biomart.cn) and maintained with Serum Free Medium for Endothelial Cells (SFM, Invitrogen) supplemented with 20 SaveFull Paper
2015Kawase, T;Tanaka, T;Okuda, K;Tsuchimochi, M;Oda, M;Hara, T;Quantitative single-cell motility analysis of platelet-rich plasma-treated endothelial cells in vitroCytoskeleton (Hoboken, N.J.)25845465HUVECs were obtained from AllCells, LLC (Emeryville, CA) and maintained in endothelial cell medium supplemented with growth factors (HuMedia-EB2; KURABO, Osaka, Japan) [Kobayashi et al., 2012]. For the wound healing assay, HUVECs were seeded into douFull Paper
2015Fung, C;Mohamad, H;Hashim, S;Htun, A;Ahmad, A;Proliferative Effect of Malaysian Propolis on Stem Cells from Human Exfoliated Deciduous Teeth: An In vitro StudyBritish Journal of Pharmaceutical ResearchThe frozen commercial SHED purchased from AllCells (USA) was retrieved and cultured in alpha minimal essential media (α-MEM) (Gibco, Invitrogen, USA) supplemented with 15% SaveFull Paper
2015Miura, Y;Ichinohe, T;Maekawa, T;Human Mesenchymal Stromal/Stem Cell-Mediated Bone Marrow OrganizationJapanese Journal of Transfusion and Cell TherapyIn this model, MSCs isolated from bone marrow samples (purchased from AllCells, Emeryville, CA, USA) through the conventional adhesion selection method (BM-MSCs, Fig. A) were SaveFull Paper
2015Kanazawa, T;Shiba, N;Aizawa, A;Okuno, H;Tamura, K;Tsukada, S;Kumamoto, T;Yamada, S;Kobayashi, Y;Arakawa, H;Nelarabine Resistance of Childhood T-Cell Lymphoblastic Leukemia/Lymphoma CellsThe Kitakanto Medical JournalIn vitro NEL resistance was seen in half of the testedchildhoodT-ALLcells. ENT-1maypartlyact as a transporter of NEL, but not play a key role in AraC induced NEL resistance. This cell SaveFull Paper
2015Wu, M;Davis, R;Hatch, A;Portable microfluidic raman system for rapid, label-free early disease signature detectionSandia National Laboratories (SNL)Order blood from Allcells(Alameda, CA), get blood within 4 hours. 2. Morning before blood arrives: 3. Make 50 ml 1x BD lysis buffer (Cat no. 349202), store at 4C. 4. Make 100X stock solutions for stimulation: 1:10 dilution of LPS stock to make 10ug/mFull Paper
2015Saka, E;Image registration and visualization of in situ gene expression images.University of LouisvilleAllcells are built out of similar materials (including an organism's DNA) and function insimilar ways. Prokaryotic cells, such as bacteria and some single celled organisms, havesimple SaveFull Paper
2015Taha, S;Ling, J;Azmi, N;Kannan, T;Azlina, A;Mokhtar, K;Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth mediumBrazilian Journal of Oral SciencesStem cells from human exfoliated deciduous teeth (SHED) (ALLCells, Alameda, CA, USA) were employed in the current study. SHED was cultured and maintained in T25 cm 2 culture SaveFull Paper
2015Neises, A;Su, R;Zhang, X;Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCsSpringerBriefs in Stem Cellsof water and add 0.9 ml of 5 % EDTA. Adjust the pH to 7.2-7.4. Add water to bring the volume up to 500 ml and filter sterilize through a 0.2-µm filter. Store at 4 °C, but bring to room temperature before use. 4. Alternatively, cord blood CD34+ cellsFull Paper
2015Doss, J;The Erythrocyte Transcriptome: Global Characterization and Therapeutic ImplicationThesisMobilized Peripheral Blood CD34+ Cells (AllCells) were grown in StemLine II media containing Glutamine (Sigma) with 20% BIT (StemCell Technologies) and 1% Penicillin/Streptomycin (Gibco). On Days 0-7, cells were supplemented with growth factors IL-3Full Paper
2015Ferng, AS;Cardiac Organogenesis: 3D Bioscaffolds, Bioenergetics and RegenerationThesisHuman amniotic stem cells were purchased (AllCells, Alameda, CA) and cultured in Celprogen media (Celprogen, Torrance, CA) under standard cell culture conditions before reseeding on the membrane patches. AmSCs were seeded at 100,000 cells/cm2 per memFull Paper
2015Quarmyne, M;Protein Tyrosine Phosphatase Receptor Type S (PTPRS) Regulates Hematopoietic Stem Cell Self-RenewalThesisCenter according to an approved protocol by the Institutional Review Board of Duke University or the Institutional Biosafety Committee of University of California - Los Angeles. Frozen CB mononuclear cells (MNCs) were obtained from AllCells, LLC., AlFull Paper
2015Sanacora, S;Regulation of IL-8 expression by proteasome in human monocytes and macrophagesThesisU937 human promyeloid cells were obtained from American Type Culture Collection (ATCC; Rockville, MD, USA). Peripheral blood mononuclear cells (PBMC) from healthy human volunteers were purchased from AllCells (PB003F; Alameda, CA, USA). The cells werFull Paper
2015Uppal, H;Doudement, E;Mahapatra, K;Potential Mechanisms for Thrombocytopenia Development with Trastuzumab Emtansine (T-DM1) The Effect of T-DM1 on Platelets and MegakaryocytesClin Cancer ResHSCs (CD133+/CD34+) were purchased from ALLCELLS. Briefly, these HSCs were enriched from bone marrow isolates of healthy donors using positive selection for CD133 and CD34. CD133+/CD34+ cells were first expanded in cytokine-enriched hematopoietic expFull Paper
2015Gupta, MS;Development of a Carboxymethylcellulose Hydrogel System for Stem Cell-Based Nucleus Pulposus Tissue EngineeringThesisHuman MSCs were isolated from the bone marrow of a 37 year-old male (AllCells, Emeryville, CA) using enriched density gradient centrifugation. Briefly, a Human MSC Enrichment Cocktail (StemCell Technologies, Vancouver, BC, Canada) (Lee et al. 2005) wFull Paper
2015Leon, B;Discovery, Development, and Applications of Natural Products: Abyssomicin 2, Quinoline Amino Alcohols, and Fluorinated Bile AcidsThesisIn vitro model of HIV latency. Generation of latent infected cells was performed as previously described.9 Briefly, naive CD4+ T cells were purified by negative selection using PBMCs from healthy HIV negative donors obtained by leukapheresis (AllCellFull Paper
2015Kizilyer, A;The Role of Neurotrophin Signaling in Megakaryocyte Differentiation and Platelet ProductionThesisCD34+ cells isolated from human bone marrow (BM) or umbilical cord blood were purchased from Allcells (Emeryville, CA, USA), and differentiated according to the previously published protocol [185]. For inducing megakaryocyte progenitor cell differentFull Paper
2015UCOK, C;KARAAHMETOĞLU, O;UCOK, C;An analysis of the decision to extract third molar teethThesisMaterials and method: SHED (AllCells, Emeryville, CA; 1 9 105 cells in 2 ml culture medium) were seeded into six-well plates and cultured for 12 h. After the change of fresh medium, LPS (E. coli; 1 lg/ml) or saline (control) was added. Following 3, 1Full Paper
2015OZMERİC KURTULUŞ, N;Selin, A;CANKAL, D;Treatment of a Patient with Unilateral Cleft Lip and Palate with Free Gingival GreftThesisMaterials and method: SHED (AllCells, Emeryville, CA; 1 9 105 cells in 2 ml culture medium) were seeded into six-well plates and cultured for 12 h. After the change of fresh medium, LPS (E. coli; 1 lg/ml) or saline (control) was added. Following 3, 1Full Paper
2015Wang, X;Wei, W;Krzeszinski, JY;Wang, Y;Wan, Y;A Liver-Bone Endocrine Relay by IGFBP1 Promotes Osteoclastogenesis and Mediates FGF21-Induced Bone ResorptionCell metabolism26456333cells were pelleted by centrifugation at 10K rpm for 10min; supernatant was collected as liver-cell-derived conditioned medium (LCM) and used to treat osteoclast differentiation culture. Human PBMN cells (ReachBio) were differentiated into osteoclastFull Paper
2015Xiang, J;Wu, DC;Chen, Y;Paulson, RF;In vitro culture of stress erythroid progenitors identifies distinct progenitor populations and analogous human progenitorsBlood25608563Human bone marrow mononuclear cells were purchased from Reachbio (#033-200) and cultured in the stress erythropoiesis medium described above except that human growth SaveFull Paper
2015Jaramillo, MC;Briehl, MM;Batinic-Haberle, I;Tome, ME;Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cellsFree radical biology & medicine25725417The manganese porphyrin, manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin (MnTE-2-PyP 5+), acts as a pro-oxidant in the presence of intracellular H 2 O 2. Mitochondria Save;Normal human peripheral mononuclear blood cells (PMBC) were pFull Paper
2015Grandi, FC;Rosser, JM;Newkirk, SJ;Yin, J;Jiang, X;Xing, Z;Whitmore, L;Bashir, S;Ivics, Z;Izsvák, Z;Ye, P;Yu, YE;An, W;Retrotransposition creates sloping shores: a graded influence of hypomethylated CpG islands on flanking CpG sitesGenome research25995269hnology, Paul Ehrlich Institute, 63225 Langen, Germany 6 These authors contributed equally to this work. 7 Present addresses: Cancer Biology Program, Stanford University, Stanford, CA 94305, USA; 8 ReachBio LLC, Seattle, WA 98107, USA; 9 DepartmenFull Paper
2015Purushothaman, P;Thakker, S;Verma, SC;Transcriptome analysis of Kaposi's sarcoma-associated herpesvirus during de novo primary infection of human B and endothelial cellsJournal of virology25552714Human PBMCs (ReachBio, WA) were cultured in RPMI 1640 medium (HyClone) supplemented with 10% FBS, 2 mM l-glutamine, and penicillin-streptomycin (5 U/ml and 5 μg/ml, respectively). Human CD14+ cells were isolated from cord blood received from the ColoFull Paper
2015Panchanathan, R;Liu, H;Leung, YK;Ho, SM;Choubey, D;Bisphenol A (BPA) stimulates the interferon signaling and activates the inflammasome activity in myeloid cellsMolecular and cellular endocrinology26277401Cells lysates were prepared and analyzed for the levels of activated caspase-1 and the mature IL-1β as described (Veeranki et al., 2011). Human purified (CD14+) monocytes from a healthy male donor were purchased from ReachBio (Seattle, WA) and culturFull Paper
2015D'Alessandro, JS;Duffner, J;Pradines, J;Capila, I;Garofalo, K;Kaundinya, G;Greenberg, BM;Kantor, D;Ganguly, TC;Equivalent Gene Expression Profiles between Glatopa™ and Copaxone®PloS one26473741tiation of the experiment to minimize bias. All samples were tested on the same day to minimize the introduction of bias resulting from sample processing. First, APC 0.5 mL (naive Balb/C splenocytes, ReachBio LLC, Seattle, WA, USA) cell suspension (1Full Paper
2015Tarrant, JM;Dhawan, P;Singh, J;Zabka, TS;Clarke, E;DosSantos, G;Dragovich, PS;Sampath, D;Lin, T;McCray, B;La, N;Nguyen, T;Kauss, A;Dambach, D;Misner, DL;Diaz, D;Uppal, H;Preclinical models of nicotinamide phosphoribosyltransferase inhibitor-mediated hematotoxicity and mitigation by co-treatment with nicotinic acidToxicology mechanisms and methods25894564Human bone marrow (n = 3) (ReachBio, Seattle, WA) or cynomolgus monkey bone marrow During the conduct of this work, authors were employees of either Genentech or ReachBio. SaveFull Paper
2015Cook, G;Studies of the efficacy of Ribonucleotide Reductase inhibition in preclinical models of Acute Myeloid Leukemia in combination with standard and novel therapeuticsThesisCells were washed with PBS and resuspended in IMDM supplemented with 20% FBS, and placed in ColonyGel High Cytokine Formulation media (ReachBio). Experiments were performed in triplicate. Colonies were counted on or after day 7. Colonies of 8 or moreFull Paper
2015Thakker, ST;Dynamics of KSHV gene expression during de novo infection and the role of LANA in immune modulationThesisErle S. Robertson, were cultured in endothelial basal medium-2 (Lonza) supplemented with endothelial growth factor supplements (Lonza). Human PBMCs (ReachBio, WA) were cultured in RPMI 1640 medium (Hyclone) supplemented with 10% FBS, 2 mM L-glutamineFull Paper
2014Li, S;Tighe, SW;Nicolet, CM;Grove, D;Levy, S;Farmerie, W;Viale, A;Wright, C;Schweitzer, PA;Gao, Y;Kim, D;Boland, J;Hicks, B;Kim, R;Chhangawala, S;Jafari, N;Raghavachari, N;Gandara, J;Garcia-Reyero, N;Hendrickson, C;Roberson, D;Rosenfeld, J;Smith, T;Underwood, JG;Wang, M;Zumbo, P;Baldwin, DA;Grills, GS;Mason, CE;Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing studyNat. Biotechnol.25150835Department of Physiology and Biophysics, Weill Cornell Medical College, New York, New York, USA.++Vermont Cancer Center, University of Vermont, Burlington, Vermont, USA.++Keck School of Medicine, University of Southern California, Los Angeles, CalifoFull Paper
2014Graves, JD;Kordich, JJ;Huang, TH;Piasecki, J;Bush, TL;Sullivan, T;Foltz, IN;Chang, W;Douangpanya, H;Dang, T;O'Neill, JW;Mallari, R;Zhao, X;Branstetter, DG;Rossi, JM;Long, AM;Huang, X;Holland, PM;Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activityCancer Cell25043603Non-small-cell lung tumor samples were collected under Institutional Review Board approval for each institution, with appropriate informed consent, from surgical resections or after biopsy. Freshly resected human tissue samples were obtained from theFull Paper
2014Brissova, M;Aamodt, K;Brahmachary, P;Prasad, N;Hong, JY;Dai, C;Mellati, M;Shostak, A;Poffenberger, G;Aramandla, R;Levy, SE;Powers, AC;Islet microenvironment, modulated by vascular endothelial growth factor-A signaling, promotes β cell regenerationCell Metab.24561261Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA. Electronic address: marcela.brissova@vanderbilt.edu.++Department of Molecular Physiology and Biophysics, VandFull Paper
2014Locke, D;Lynch, F;Bernstein, S;Siami-Namini, K;Yarranton, G;An IHC Screen for EphA3 Positive Myelofibrosis (MF) and Other Myeloproliferative Neoplasms (MPNs)BloodNeoplasms (MPNs). Darren Locke, PhD Darren Locke, PhD. 1. QualTek Molecular Laboratories, Newtown,. 1. Search for other works by this author on: This Site. PubMed MPNs. Disclosures. Locke:QualTek Molecular Labs: Employment. LynchFull Paper
2014Fecteau, JF;Corral, LG;Ghia, EM;Gaidarova, S;Futalan, D;Bharati, IS;Cathers, B;Schwaederlé, M;Cui, B;Lopez-Girona, A;Messmer, D;Kipps, TJ;Lenalidomide inhibits the proliferation of CLL cells via a cereblon/p21(WAF1/Cip1)-dependent mechanism independent of functional p53Blood24990888Blood samples were collected from CLL patients at the University of California San Diego Moores Cancer Center who satisfied diagnostic and immunophenotypic criteria for common B-cell CLL, and who provided written, informed consent, in compliance withFull Paper
2014Sugihara, K;Kobayashi, Y;Suzuki, A;Tamura, N;Motamedchaboki, K;Huang, CT;Akama, TO;Pecotte, J;Frost, P;Bauer, C;Jimenez, JB;Nakayama, J;Aoki, D;Fukuda, MN;Development of pro-apoptotic peptides as potential therapy for peritoneal endometriosisNat Commun25047118... wa (positive) and A431 (negative) cells. A hybridoma clone was subcloned by a limited dilution, establishing the 3B2 clone. Paraffin tissue sections endometriosis patients (_n_=35) were obtained from Folio Biosciences (Columbus, OH). A normal uteFull Paper
2014Ramesh, R;Kozhaya, L;McKevitt, K;Djuretic, IM;Carlson, TJ;Quintero, MA;McCauley, JL;Abreu, MT;Unutmaz, D;Sundrud, MS;Pro-inflammatory human Th17 cells selectively express P-glycoprotein and are refractory to glucocorticoidsJ. Exp. Med.24395888... sue samples. All experiments using human blood and tissue samples were conducted in accordance with IRB protocols approved by committees at Research Blood Components, New York Blood Center, OneBlood, Conversant Bio., and the University of Miami.Full Paper
2014Van Bortle, K;Nichols, MH;Li, L;Ong, CT;Takenaka, N;Qin, ZS;Corces, VG;Insulator function and topological domain border strength scale with architectural protein occupancyGenome Biol.24981874We are especially thankful to Drs Dale Dorsett, Hugo Bellen, Giovanni Bosco, and Jørgen Johansen for graciously sharing antibodies against Rad21, Barren, CAP-H2, and Chromator. We thank the Drosophila Genomic Resource Center (supported by NIH grant OFull Paper
2014Steffen, MM;Dearth, SP;Dill, BD;Li, Z;Larsen, KM;Campagna, SR;Wilhelm, SW;Nutrients drive transcriptional changes that maintain metabolic homeostasis but alter genome architecture in MicrocystisISME J24858783; Scientific, Waltham, MA, USA). Subsequent sample processing, including rRNA subtraction, library prep and sequencing, were performed by the HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA). RNA quality wasFull Paper
2014Picard, M;Zhang, J;Hancock, S;Derbeneva, O;Golhar, R;Golik, P;O'Hearn, S;Levy, S;Potluri, P;Lvova, M;Davila, A;Lin, CS;Perin, JC;Rappaport, EF;Hakonarson, H;Trounce, IA;Procaccio, V;Wallace, DC;Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional reprogrammingProc. Natl. Acad. Sci. U.S.A.25192935Center for Mitochondrial and Epigenomic Medicine, Children's Hospital of Philadelphia and the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104;++School of Biological Sciences, The University of HongFull Paper
2014Smith, DC;Eisenberg, PD;Manikhas, G;Chugh, R;Gubens, MA;Stagg, RJ;Kapoun, AM;Xu, L;Dupont, J;Sikic, B;A phase I dose escalation and expansion study of the anticancer stem cell agent demcizumab (anti-DLL4) in patients with previously treated solid tumorsClin. Cancer Res.25324140In addition, control plasma and whole blood samples were sourced and collected (Conversant Bio) using the same methods described above from 8 cancer patients who were not treated with demcizumab. Affymetrix human gene chip U133 Plus 2.0 arrays were uFull Paper
2014Gee, HY;Ashraf, S;Wan, X;Vega-Warner, V;Esteve-Rudd, J;Lovric, S;Fang, H;Hurd, TW;Sadowski, CE;Allen, SJ;Otto, EA;Korkmaz, E;Washburn, J;Levy, S;Williams, DS;Bakkaloglu, SA;Zolotnitskaya, A;Ozaltin, F;Zhou, W;Hildebrandt, F;Mutations in EMP2 cause childhood-onset nephrotic syndromeAm. J. Hum. Genet.24814193Division of Nephrology, Department of Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA.++Division of Nephrology, Department of Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USFull Paper
2014Gee, HY;Otto, EA;Hurd, TW;Ashraf, S;Chaki, M;Cluckey, A;Vega-Warner, V;Saisawat, P;Diaz, KA;Fang, H;Kohl, S;Allen, SJ;Airik, R;Zhou, W;Ramaswami, G;Janssen, S;Fu, C;Innis, JL;Weber, S;Vester, U;Davis, EE;Katsanis, N;Fathy, HM;Jeck, N;Klaus, G;Nayir, A;Rahim, KA;Al Attrach, I;Al Hassoun, I;Ozturk, S;Drozdz, D;Helmchen, U;O'Toole, JF;Attanasio, M;Lewis, RA;Nürnberg, G;Nürnberg, P;Washburn, J;MacDonald, J;Innis, JW;Levy, S;Hildebrandt, F;Whole-exome resequencing distinguishes cystic kidney diseases from phenocopies in renal ciliopathiesKidney Int.24257694Division of Nephrology, Department of Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.++Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, USA.++MRC Human GeneticsFull Paper
2014Rosenfeldt, MT;Bell, LA;Long, JS;O'Prey, J;Nixon, C;Roberts, F;Dufès, C;Ryan, KM;E2F1 drives chemotherapeutic drug resistance via ABCG2Oncogene24276245Biotechnology (St Louis, MO, USA). The ChIP Assay Kit was from Millipore (Watford, UK) and the human lung cancer tissue microarray was from Folio Biosciences (Columbus, OH, USA). Plasmids. pTRE-E2F1 constructs were generatedFull Paper
2014Wildsmith, KR;Schauer, SP;Smith, AM;Arnott, D;Zhu, Y;Haznedar, J;Kaur, S;Mathews, WR;Honigberg, LA;Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomicsMol Neurodegener24902845... ap in clinical biomarker development. Methods Source of CSF CSF was purchased from Bioreclamation, LLC (Hicksville, NY) (pooled cynomolgus monkey and pooled human CSF used in discovery experiments), Folio Biosciences (Columbus, OH) (individual lFull Paper
2014Farber, CR;Reich, A;Barnes, AM;Becerra, P;Rauch, F;Cabral, WA;Bae, A;Quinlan, A;Glorieux, FH;Clemens, TL;Marini, JC;A novel IFITM5 mutation in severe atypical osteogenesis imperfecta type VI impairs osteoblast production of pigment epithelium-derived factorJ. Bone Miner. Res.24519609Exome sequencing was performed by the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology (Huntsville, AL, USA). Briefly, gDNA (1 to 2 μg) was fragmented and subjected to exome enrichment using the Nimblegen SeqCap EZ Human Exome LibrFull Paper
2014Shipitsin, M;Small, C;Choudhury, S;Giladi, E;Friedlander, S;Nardone, J;Hussain, S;Hurley, AD;Ernst, C;Huang, YE;Chang, H;Nifong, TP;Rimm, DL;Dunyak, J;Loda, M;Berman, DM;Blume-Jensen, P;Identification of proteomic biomarkers predicting prostate cancer aggressiveness and lethality despite biopsy-sampling errorBr. J. Cancer25032733Acquisition, processing, quality control, and annotation of FFPE prostate cancer tissue blocks. A set of FFPE human prostate cancer tissue blocks with clinical annotations and long-term patient outcome information was acquired from Folio BiosciencesFull Paper
2014Hermetz, KE;Newman, S;Conneely, KN;Martin, CL;Ballif, BC;Shaffer, LG;Cody, JD;Rudd, MK;Large inverted duplications in the human genome form via a fold-back mechanismPLoS Genet.24497845SureSelect capture and Illumina HiSeq sequencing were performed at Hudson Alpha Genomic Services Lab (http://www.hudsonalpha.org/gsl/). After NGS, we aligned 100-bp paired-end reads from fastq files to the GRC37/hg19 reference genome using Burrows-WhFull Paper
2014Campbell, VT;Nadesan, P;Ali, SA;Wang, CY;Whetstone, H;Poon, R;Wei, Q;Keilty, J;Proctor, J;Wang, LW;Apte, SS;McGovern, K;Alman, BA;Wunder, JS;Hedgehog pathway inhibition in chondrosarcoma using the smoothened inhibitor IPI-926 directly inhibits sarcoma cell growthMol. Cancer Ther.24634412Formalin-fixed, paraffin-embedded human chondrosarcoma tissue microarrays (TMA) were purchased from Folio Biosciences for cilia staining. Before staining, all slides were placed in a 60 C oven and baked for 1 hour. Sections were deparaffinized in xylFull Paper
2014Folcik, VA;Garofalo, M;Coleman, J;Donegan, JJ;Rabbani, E;Suster, S;Nuovo, A;Magro, CM;Di Leva, G;Nuovo, GJ;Idiopathic pulmonary fibrosis is strongly associated with productive infection by herpesvirus saimiriMod. Pathol.242328641] The Ohio State University (OSU) at Marion, Marion, OH, USA [2] OSU Computer Science and Engineering, Marion, OH, USA [3] OSU Innovation Group for the Study of Complexity in Human, Natural, and Engineered Systems, Marion, OH, USA.++Satellite LaboraFull Paper
2014Lee, CW;Zhan, Q;Lezcano, C;Frank, MH;Huang, J;Larson, AR;Lin, JY;Wan, MT;Lin, PI;Ma, J;Kleffel, S;Schatton, T;Lian, CG;Murphy, GF;Nestin depletion induces melanoma matrix metalloproteinases and invasionLab. Invest.25365206An additional 153 cases of primary and metastatic melanomas demonstrating clear nodular or infiltrative growth patterns were evaluated for nestin expression in melanoma tissue microarrays (TMA) purchased from Folio Bio (Powell, OH, USA), US Biomax (RFull Paper
2014Johansson, H;Orjalo, A;Coassin, S;Yin, R;Abstract 3399: Detection and validation of novel RNA cancer biomarkers by single molecule RNA fluorescence in situ hybridization (smRNA FISH)Molecular and Cellular BiologyMethods Mol Biol. Ron Cook - Founder, President and CEO Marc Beal - Director of Corporate Development Peter Coassin - Director of Advanced Technology Development Entire Stellaris Team OPKOLab Folio Biosciences #3399 A B A B SK-BR-3 MCF7 T47DFull Paper
2014Hornby, PJ;Cooper, PR;Kliwinski, C;Ragwan, E;Mabus, JR;Harman, B;Thompson, S;Kauffman, AL;Yan, Z;Tam, SH;Dorai, H;Powers, GD;Giles-Komar, J;Human and non-human primate intestinal FcRn expression and immunoglobulin G transcytosisPharm. Res.24072267Paraffin-imbedded 5 μm sections were cut from full-thickness intestine harvested from monkey and human donors, and from human tissue bank (MTB-311 and MTB-316; Qualtek Molecular Labs, Goleta, CA) and dried on glass slides (60°C). Steam heat induced eFull Paper
2014Jane, EP;Premkumar, DR;Morales, A;Foster, KA;Pollack, IF;Inhibition of phosphatidylinositol 3-kinase/AKT signaling by NVP-BKM120 promotes ABT-737-induced toxicity in a caspase-dependent manner through mitochondrial dysfunction and DNA damage response in established and primary cultured glioblastoma cellsJ. Pharmacol. Exp. Ther.24741074The human glioma cell lines U87 (PTEN deleted, p53 wild type) and T98G (PTEN mutant, p53 mutant) were purchased from the ATCC (Manassas, VA). LN18 (PTEN wild type, p53 mutant), LN229 (PTEN wild type, p53 mutant), and LNZ308 (PTEN deleted, p53 deletedFull Paper
2014Hassan, F;Xu, X;Nuovo, G;Killilea, DW;Tyrrell, J;Da Tan, C;Tarran, R;Diaz, P;Jee, J;Knoell, D;Boyaka, PN;Cormet-Boyaka, E;Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levelsRespir. Res.24957904... ell@osumc.edu Boyaka Prosper N 1 boyaka.1@osu.edu Cormet-Boyaka Estelle 1 boyaka.2@osu.edu 1Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA 2Phylogeny Inc., Columbus, OH, USA 3Nutrition aFull Paper
2014Burger, KL;Learman, BS;Boucherle, AK;Sirintrapun, SJ;Isom, S;Díaz, B;Courtneidge, SA;Seals, DF;Src-dependent Tks5 phosphorylation regulates invadopodia-associated invasion in prostate cancer cellsProstate24174371To conduct an immunohistochemical analysis of Tks5 protein, we purchased a high density prostate tumor microarray (Folio Bioscience, Powell, OH) containing 92, duplicate cases of formalin‐fixed human prostatic adenocarcinoma and 20 cases of normal anFull Paper
2014Gómez-Díaz, E;Rivero, A;Chandre, F;Corces, VG;Insights into the epigenomic landscape of the human malaria vector Anopheles gambiaeFront Genet25177345Total RNA was extracted from fresh mosquito tissues (~25 midguts and ~ 50 salivary glands) using the mirVana™ RNA Isolation Kit (Ambion®) according to the manufacturer's protocol. RNA concentration was quantified using a Qubit® 2.0 Fluorometer, and RFull Paper
2014Xu, J;Zhao, Z;Zhang, X;Zheng, X;Li, J;Jiang, Y;Kuang, Y;Zhang, Y;Feng, J;Li, C;Yu, J;Li, Q;Zhu, Y;Liu, Y;Xu, P;Sun, X;Development and evaluation of the first high-throughput SNP array for common carp (Cyprinus carpio)BMC Genomics24762296;... es were collected and DNA was extracted using a DNeasy Blood & Tissue Kit (Qiagen, Shanghai, China). The samples are listed in Table 1. DNA library preparation and sequencing were carried out at the HudsonAlpha Genomic Services Laboratory (HuntsFull Paper
2014Gruver, AM;Liu, L;Vaillancourt, P;Yan, SC;Cook, JD;Roseberry Baker, JA;Felke, EM;Lacy, ME;Marchal, CC;Szpurka, H;Holzer, TR;Rhoads, EK;Zeng, W;Wortinger, MA;Lu, J;Chow, CK;Denning, IJ;Beuerlein, G;Davies, J;Hanson, JC;Credille, KM;Wijayawardana, SR;Schade, AE;Immunohistochemical application of a highly sensitive and specific murine monoclonal antibody recognising the extracellular domain of the human hepatocyte growth factor receptor (MET)Histopathology25039923both acquired from US Biomax, Rockville, MD, USA), and 67 NSCLC whole‐tissue specimens purchased from Asterand (Detroit, MI, USA), US Biomax, Capital Biosciences, Cybridi (Gaithersburg, MD, USA), Indivumed (Baltimore, MD, USA), Conversant Bio (HuntsvFull Paper
2014Li, C;Beck, BH;Peatman, E;Nutritional impacts on gene expression in the surface mucosa of blue catfish (Ictalurus furcatus)Dev. Comp. Immunol.24378224; For each timepoint, equal amounts of RNA from skin and gill were pooled together to generate 3 pooled replicates for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (HunFull Paper
2014Ehrhardt, N;Bedoya, C;Péterfy, M;Embryonic viability, lipase deficiency, hypertriglyceridemia and neonatal lethality in a novel LMF1-deficient mouse modelNutr Metab (Lond)25302068RT-PCR. p6. CGTGGAGCTTCTTGTGCCT. qPCR. p7. GGATCTGCAGCACTCCAT. qPCR. 36B4-f. CACTGGTCTAGGACCCGAGAAG. qPCR. 36B4-r. GGTGCCTCTGGAGATTTTCG. qPCR. RNA analysis. In situ hybridization was carried out by Phylogeny Inc. as described [11]Full Paper
2014Choudhary, A;Mambo, E;Sanford, T;Boedigheimer, M;Twomey, B;Califano, J;Hadd, A;Oliner, KS;Beaudenon, S;Latham, GJ;Adai, AT;Evaluation of an integrated clinical workflow for targeted next-generation sequencing of low-quality tumor DNA using a 51-gene enrichment panelBMC Med Genomics25395014The 26 tumor specimens were all colon or rectal adenocarcinomas obtained from Asterand (Detroit, MI, USA). The remaining colon cancer FFPE tissue specimens come from stage II tumors that were acquired from FolioBio (Columbus, OH)Full Paper
2014Stavropoulos, SW;Sing, RF;Elmasri, F;Silver, MJ;Powell, A;Lynch, FC;Aal, AK;Lansky, AJ;Settlage, RA;Muhs, BE;, ;The DENALI Trial: an interim analysis of a prospective, multicenter study of the Denali retrievable inferior vena cava filterJ Vasc Interv Radiol25066514Full Paper
2014Day, K;Song, J;Absher, D;Targeted sequencing of large genomic regions with CATCH-SeqPLoS ONE25357200HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United States of America.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United States of America.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United StaFull Paper
2014Sun, L;Liu, S;Wang, R;Jiang, Y;Zhang, Y;Zhang, J;Bao, L;Kaltenboeck, L;Dunham, R;Waldbieser, G;Liu, Z;Identification and analysis of genome-wide SNPs provide insight into signatures of selection and domestication in channel catfish (Ictalurus punctatus)PLoS ONE25313648; protocol. Equal amounts of DNA (100 µg) from each individual were pooled for sequencing, one pool for each strain. Sequencing was conducted commercially at HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). GenomicFull Paper
2014Jiang, Y;Zhang, S;Xu, J;Feng, J;Mahboob, S;Al-Ghanim, KA;Sun, X;Xu, P;Comparative transcriptome analysis reveals the genetic basis of skin color variation in common carpPLoS ONE25255374;... nufacturer’s instructions. RNA quality was verified using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Equal amounts of high-quality RNA from each tissue were pooled and sent to HudsonAlpha Genomic Services Laboratory (HuntsvFull Paper
2014Jun, LS;Showalter, AD;Ali, N;Dai, F;Ma, W;Coskun, T;Ficorilli, JV;Wheeler, MB;Michael, MD;Sloop, KW;A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptorPLoS ONE24695667For IHC, tissues and cells were fixed and processed for paraffin embedding using routine procedures as described previously [30]. Paraffin-embedded human pancreas samples were purchased from Folio Biosciences (Columbus, OH). IP-WB and mRNA ExpressionFull Paper
2014Luo, Y;Li, C;Landis, AG;Wang, G;Stoeckel, J;Peatman, E;Transcriptomic profiling of differential responses to drought in two freshwater mussel species, the giant floater Pyganodon grandis and the pondhorn Uniomerus tetralasmusPLoS ONE24586812; construction, Illumina sequencing and expression analysis. RNA-Seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were constructed withFull Paper
2014Li, SZ;Hu, YY;Zhao, J;Zhao, YB;Sun, JD;Yang, YF;Ji, CC;Liu, ZB;Cao, WD;Qu, Y;Liu, WP;Cheng, G;Fei, Z;MicroRNA-34a induces apoptosis in the human glioma cell line, A172, through enhanced ROS production and NOX2 expressionBiochem. Biophys. Res. Commun.243938442. Materials and methods. 2.1. Human samples and cell lines. Human glioma cancer tissue microarrays (Lot Number: ARY-HH0118-011-02) containing 60 glioma cancer specimens and 30 normal tissues were purchased from Folio Biosciences (Columbus, OH, USA)Full Paper
2014Yim, KM;Brewer, MS;Miller, CT;Gillespie, RG;Comparative transcriptomics of maturity-associated color change in Hawaiian spidersJ. Hered.25149253Extractions were shipped to the Genomic Services Lab at HudsonAlpha (Huntsville, AL) for library preparation and sequencing. Complementary DNA libraries were prepared from total RNA, and 50bp paired-end Illumina HiSeq 2000 RNA-seq was used to generatFull Paper
2014Shipitsin, M;Small, C;Giladi, E;Siddiqui, S;Choudhury, S;Hussain, S;Huang, YE;Chang, H;Rimm, DL;Berman, DM;Nifong, TP;Blume-Jensen, P;Automated quantitative multiplex immunofluorescence in situ imaging identifies phospho-S6 and phospho-PRAS40 as predictive protein biomarkers for prostate cancer lethalityProteome Sci25075204In total, we procured and tested 508 unique prostatectomy samples with lethal outcome annotation available (Folio Biosciences, Powell, OH). Of these, 418 passed quality testing and were used for our TMA (Additional file 1: Table S2). Figure 2 FigureFull Paper
2014Drogemöller, BI;Niehaus, DJ;Chiliza, B;van der Merwe, L;Asmal, L;Malhotra, AK;Wright, GE;Emsley, R;Warnich, L;Patterns of variation influencing antipsychotic treatment outcomes in South African first-episode schizophrenia patientsPharmacogenomics24444409gDNA from the 11 individuals was extracted from venous blood using the QIAamp DNA Blood Maxi Kit (Qiagen, Germany), accord - ing to the manufacturer’s instructions. There - after, the gDNA samples were sent to the HudsonAlpha Genomics Service LaboraFull Paper
2014Zhang, J;Vernes, JM;Ni, J;Nelson, C;Wong, A;Chen, ST;Asundi, A;Vandlen, R;Meng, YG;Real-time immuno-polymerase chain reaction in a 384-well format: detection of vascular endothelial growth factor and epidermal growth factor-like domain 7Anal. Biochem.25013989CAL-12T cell line was purchased from German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). Human cancer tissues were purchased from ConversantBio (Huntsville, AL) or Avaden BioSciences (Scarsdale, NY)Full Paper
2014Aycock, KI;Campbell, RL;Manning, KB;Sastry, SP;Shontz, SM;Lynch, FC;Craven, BA;A computational method for predicting inferior vena cava filter performance on a patient-specific basisJ Biomech Eng24805200Full Paper
2014Sarran-Armogan, L;Prospective isolation of human myeloerythroid progenitors in fetal liver and embryonic stem cell differentiation culturesThesisEinstein Fetal Tissue repository under IRB approved protocol. Human cord blood was obtained from the Obstetric Department at the Weiler Hospital of the Albert Einstein College of Medicine under an IRB approved protocol. Human bone marrow CD34+ cellsFull Paper
2014Miyake, M;Lawton, A;Goodison, S;Urquidi, V;Rosser, CJ;Chemokine (C-X-C motif) ligand 1 (CXCL1) protein expression is increased in high-grade prostate cancerPathol. Res. Pract.24252309High-density commercial tissue microarrays (TMA) (ARY-HH0185, Foliobio, Powell, OH) constructed from formalin fixed, paraffin embedded clinical samples comprised the cohort of 110 patients (90 prostate cancer and 20 benign prostates without evidenceFull Paper
2014Pollen, AA;Nowakowski, TJ;Shuga, J;Wang, X;Leyrat, AA;Lui, JH;Li, N;Szpankowski, L;Fowler, B;Chen, P;Ramalingam, N;Sun, G;Thu, M;Norris, M;Lebofsky, R;Toppani, D;Kemp, DW;Wong, M;Clerkson, B;Jones, BN;Wu, S;Knutsson, L;Alvarado, B;Wang, J;Weaver, LS;May, AP;Jones, RC;Unger, MA;Kriegstein, AR;West, JA;Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortexNature biotechnology25086649For systems verification tests of capture efficiency, primary cells were obtained from splenocytes (AllCells, LLC PB003F) and peripheral blood mononuclear cells (AllCells, LLC PB003F) SaveFull Paper
2014Cohen, A;Regenerative potential of human CD34+ stem cells mobilized and enriched from bone marrowThesisselected from granulocyte colony-stimulating factor (G-CSF) (Amgen; Thousand Oaks, CA, USA) mobilized peripheral blood mononuclear cells (mPBMNCs) obtained from AllCells (Regimen E, Emeryville, CA). Healthy human donors were injected with G-CSF onceFull Paper
2014Koellmer, M;Multilineage differentiation of mesenchymal stem cells for cell-based tissue engineeringThesisHuman bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque TM PLUS solution and RosetteSep® Human Mesenchymal Stem Cell Enrichment Cocktail (Stem Cell TechnologFull Paper
2014Lu, M;Xia, L;Li, Y;Wang, X;Hoffman, R;The orally bioavailable MDM2 antagonist RG7112 and pegylated interferon α 2a target JAK2V617F-positive progenitor and stem cellsBlood24869939human CD34+ cell selection kit (StemCell Technologies, Vancouver, BC, Canada). Normal human bone marrow (BM) mononuclear cells and CD34+ cells were purchased from AllCells (Emeryville, CA). The experiments for which the CD34+ cells from each of the 3Full Paper
2014Shi, J;Rose, EL;Singh, A;Hussain, S;Stagliano, NE;Parry, GC;Panicker, S;TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutininsBlood24695853Human RBCs (hRBCs; blood type O negative; Allcells, Alameda, CA) were washed 3 times with gelatin veronal buffer (GVB; Complement Technology, Tyler, TX) and resuspended at 1 × 109 cells per milliliter in GVB plus 10 mM EDTA. To sensitize hRBCs with CFull Paper
2014Adamia, S;Bar-Natan, M;Haibe-Kains, B;Pilarski, PM;Bach, C;Pevzner, S;Calimeri, T;Avet-Loiseau, H;Lode, L;Verselis, S;Fox, EA;Galinsky, I;Mathews, S;Dagogo-Jack, I;Wadleigh, M;Steensma, DP;Motyckova, G;Deangelo, DJ;Quackenbush, J;Tenen, DG;Stone, RM;Griffin, JD;NOTCH2 and FLT3 gene mis-splicings are common events in patients with acute myeloid leukemia (AML): new potential targets in AMLBlood24574459Our previous studies revealed an increase in alternative splicing of multiple RNAs in cells from patients with acute myeloid leukemia (AML) compared with CD34 + bone marrow cells SaveFull Paper
2014Krow-Lucal, ER;Kim, CC;Burt, TD;McCune, JM;Distinct functional programming of human fetal and adult monocytesBlood24518760Adult bone marrow samples were obtained from healthy donors (AllCells and Lonza Group). Both adult and fetal mononuclear cells were isolated by density centrifugation of a Ficoll- SaveFull Paper
2014Shi, Q;Kuether, EL;Chen, Y;Schroeder, JA;Fahs, SA;Montgomery, RR;Platelet gene therapy corrects the hemophilic phenotype in immunocompromised hemophilia A mice transplanted with genetically manipulated human cord blood stem cellsBlood24269957Human cord blood (hCB) CD34+ cells were purchased from AllCells (Emeryville, CA) and transduced with 2bF8LV by using a protocol similar to that described in previous reports.10,15 The details are provided in the supplemental Data (available on the BlFull Paper
2014Thompson, A;Rasko, J;Hongeng, S;Kwiatkowski, J;Schiller, G;von Kalle, C;Cavazzana, M;Leboulch, P;Petrusich, A;Soni, S;Walters, M;Initial Results from the Northstar Study (HGB-204): A Phase 1/2 Study of Gene Therapy for β-Thalassemia Major Via Transplantation of Autologous Hematopoietic Stem Cells Transduced Ex Vivo with a Lentiviral βΑ-T87Q -Globin Vector (LentiGlobin BB305 Drug Product)BloodBackground: Hematopoietic stem cell (HSC) gene therapy has the potential to induce globin production and mitigate the need for blood transfusions in β-thalassemia major. Promising early results for 2 subjects with β0/βE -thalassemia major in the ongoFull Paper
2014Iwasa, M;Miura, Y;Fujishiro, A;Tamura, A;Sato, A;Yokota, A;Nakagawa, Y;Yoshioka, S;Hirai, H;Andoh, A;Ichinohe, T;Maekawa, T;Effects of Irradiation on the Functional Characteristics of Human Bone Marrow Mesenchymal Stromal/Stem CellsBloodBM samples were purchased from AllCells (Emeryville, CA). For the irradiation experiments, BM-MSCs were g-irradiated (Cesium-137) with various doses ranged from 2 to 12 Gy by a Save;Total-body irradiation is frequently used as a conditioning treatmFull Paper
2014Sahler, J;Modulation of Transcription Factors in Platelets and Platelet Microparticles Attenuates Inflammatory ResponsesThesisAdapted from previous procedures, 94 human CD34+ cord blood cells were purchased from Allcells (Emeryville, CA) and first plated at 2.5x105 cells/mL in IMDM supplemented with 20% BIT 9500, 0.8% Low Density Lipoprotein (L-8292, Sigma), 100ng/mL recombFull Paper
2014Li, S;Garrett-Bakelman, F;Perl, AE;Luger, SM;Zhang, C;To, BL;Lewis, ID;Brown, AL;D'Andrea, RJ;Ross, ME;Levine, R;Carroll, M;Melnick, A;Mason, CE;Dynamic evolution of clonal epialleles revealed by methcloneGenome biology25260792samples at two time points (diagnosis and relapse stages), enriched for myeloblast cells, were used in the experiments, and these samples were also compared to five CD34+ normal bone marrow samples (AllCells, Inc.). Institutional review board approvaFull Paper
2014Bartholdy, B;Christopeit, M;Will, B;Mo, Y;Barreyro, L;Yu, Y;Bhagat, TD;Okoye-Okafor, UC;Todorova, TI;Greally, JM;Levine, RL;Melnick, A;Verma, A;Steidl, U;HSC commitment-associated epigenetic signature is prognostic in acute myeloid leukemiaThe Journal of clinical investigation24487588Acute myeloid leukemia (AML) is characterized by disruption of HSC and progenitor cell differentiation. Frequently, AML is associated with mutations in genes encoding epigenetic SaveFull Paper
2014Schlinker, AC;In Vitro Platelet Production: Promoting Megakaryocyte Terminal Differentiation, Demonstrating Platelet Functionality, and Developing an Automated Process for Platelet HarvestThesisFigure 4.1. PLT-like particle (PLP) and proPLT production in X-VIVO 10 cultures. (A) Blood PLTs obtained from AllCells (top panels) and PLPs exposed to shear for 3 days after CD61+ selection on day 10 (bottom panels) were stained with anti-CD41 and aFull Paper
2014Accomando, WP;Wiencke, JK;Houseman, EA;Nelson, HH;Kelsey, KT;Quantitative reconstruction of leukocyte subsets using DNA methylationGenome biology24598480ummarizes the workflow carried out for all samples, derived from human whole blood, that were utilized in this work. Blood was obtained through an institutional review board-approved donor program at AllCells, LLC (Alameda, CA, USA) and research wasFull Paper
2014Liu, GH;Suzuki, K;Li, M;Qu, J;Montserrat, N;Tarantino, C;Gu, Y;Yi, F;Xu, X;Zhang, W;Ruiz, S;Plongthongkum, N;Zhang, K;Masuda, S;Nivet, E;Tsunekawa, Y;Soligalla, RD;Goebl, A;Aizawa, E;Kim, NY;Kim, J;Dubova, I;Li, Y;Ren, R;Benner, C;Del Sol, A;Bueren, J;Trujillo, JP;Surralles, J;Cappelli, E;Dufour, C;Esteban, CR;Belmonte, JCI;Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCsNature communications24999918Human BM CD34 + cells from a healthy individual were purchased from Allcells (Alameda, CA). FA patient BM cells were isolated from two FA patients bearing FANCA mutations ( SaveFull Paper
2014Green, MR;Vicente-Dueñas, C;Romero-Camarero, I;Long Liu, C;Dai, B;González-Herrero, I;García-Ramírez, I;Alonso-Escudero, E;Iqbal, J;Chan, WC;Campos-Sanchez, E;Orfao, A;Pintado, B;Flores, T;Blanco, O;Jiménez, R;Martínez-Climent, JA;Criado, FJ;Cenador, MB;Zhao, S;Natkunam, Y;Lossos, IS;Majeti, R;Melnick, A;Cobaleda, C;Alizadeh, AA;Sánchez-García, I;Transient expression of Bcl6 is sufficient for oncogenic function and induction of mature B-cell lymphomaNature communications24887457Bone marrow from two healthy donors (1 male, 1 female) were obtained purchased from AllCells, LLC. (Emeryville, CA) and sorted on a FACS ARIA II instrument as PI−, Lin−, CD34+, CD38−. Single cells were sorted into Terasaki plates containing alternatiFull Paper
2014Minn, I;Wang, H;Mease, RC;Byun, Y;Yang, X;Wang, J;Leach, SD;Pomper, MG;A red-shifted fluorescent substrate for aldehyde dehydrogenaseNature communications24759454(BD Biosciences, San Jose, CA) with 488 nm blue laser and phycoerythrin (PE) filters. Isolation of ALDHhi human hematopoietic stem cells Frozen human cord blood mononuclear cells were purchased from ALLCELLS (Alamenda, CA). Cells were thawed accordiFull Paper
2014Verma, R;Su, S;McCrann, DJ;Green, JM;Leu, K;Young, PR;Schatz, PJ;Silva, JC;Stokes, MP;Wojchowski, DM;RHEX, a novel regulator of human erythroid progenitor cell expansion and erythroblast developmentThe Journal of experimental medicine25092874O (and cytokine) challenges were at the concentrations and time intervals indicated. Primary human hematopoietic cells used in RT-PCR analyses included monocytes, neutrophils, T cells, and platelets (AllCells). Murine G1E-ER4 cells (M. Weiss, ChildreFull Paper
2014Roche, ET;Hastings, CL;Lewin, SA;Shvartsman, D;Brudno, Y;Vasilyev, NV;O'Brien, FJ;Walsh, CJ;Duffy, GP;Mooney, DJ;Comparison of biomaterial delivery vehicles for improving acute retention of stem cells in the infarcted heartBiomaterials24862441hMSCs (AllCells, USA) were encapsulated in chitosan/β-GP or alginate gels at a density of 100,000 cells/100μL gel (1 × 106 cells/mL) and 100μL was added to a hanging well cell culture insert with a pore size of 1μm, suitable for insertion into a 48-wFull Paper
2014Adamia, S;Haibe-Kains, B;Pilarski, PM;Bar-Natan, M;Pevzner, S;Avet-Loiseau, H;Lode, L;Verselis, S;Fox, EA;Burke, J;Galinsky, I;Dagogo-Jack, I;Wadleigh, M;Steensma, DP;Motyckova, G;Deangelo, DJ;Quackenbush, J;Stone, R;Griffin, JD;A genome-wide aberrant RNA splicing in patients with acute myeloid leukemia identifies novel potential disease markers and therapeutic targetsClinical cancer research : an official journal of the American Association for Cancer Research24284058A control group of 12 normal donors of both genders was also included: 8 bone marrow samples of normal donors were obtained from AllCells (California) and 4 peripheral blood SaveFull Paper
2014Wang, X;Moghimi, B;Zolotukhin, I;Morel, LM;Cao, O;Herzog, RW;Immune tolerance induction to factor IX through B cell gene transfer: TLR9 signaling delineates between tolerogenic and immunogenic B cellsMolecular therapy : the journal of the American Society of Gene Therapy24609143Primary B cells derived from healthy humans were purchased from Allcells (Alameda, CA). Cell culture reagents were purchased from Invitrogen (Grand Island, NY). Recombinant SaveFull Paper
2014Carbonaro, DA;Zhang, L;Jin, X;Montiel-Equihua, C;Geiger, S;Carmo, M;Cooper, A;Fairbanks, L;Kaufman, ML;Sebire, NJ;Hollis, RP;Blundell, MP;Senadheera, S;Fu, PY;Sahaghian, A;Chan, RY;Wang, X;Cornetta, K;Thrasher, AJ;Kohn, DB;Gaspar, HB;Preclinical demonstration of lentiviral vector-mediated correction of immunological and metabolic abnormalities in models of adenosine deaminase deficiencyMolecular therapy : the journal of the American Society of Gene Therapy24256635Normal human adult bone marrow samples (100 ml/donor) were also purchased from AllCells, LLC (Emeryville, CA). Human CD34+ cell isolation was performed as described.51 Briefly, human cord blood or human bone marrow was diluted 1:2 with Dulbecco's phoFull Paper
2014Huang, X;Shah, S;Wang, J;Ye, Z;Dowey, SN;Tsang, KM;Mendelsohn, LG;Kato, GJ;Kickler, TS;Cheng, L;Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factorsMolecular therapy : the journal of the American Society of Gene Therapy24002691Johns Hopkins University Institutional Review Board. Human primary CB MNCs are obtained from anonymous, healthy donors and then isolated in our lab using Ficoll-Paque Plus (P = 1.077) or directly purchased from AllCells, LLC (AllCells, Emeryville, CAFull Paper
2014Chorzalska, A;Salloum, I;Shafqat, H;Khan, S;Marjon, P;Treaba, D;Schorl, C;Morgan, J;Bryke, CR;Falanga, V;Zhao, TC;Reagan, J;Winer, E;Olszewski, AJ;Al-Homsi, AS;Kouttab, N;Dubielecka, PM;Low expression of Abelson interactor-1 is linked to acquired drug resistance in Bcr-Abl-induced leukemiaLeukemia24699303cross-talk may be causative in the acquired drug resistant phenotype of LSCs. Materials and methods Patients and donors Human CD34+ cells were isolated from bone marrow (BM) mononuclear cells (MNC) (AllCells, LLC, Emeryville, CA) or from peripheralFull Paper
2014Yang, H;Bueso-Ramos, C;DiNardo, C;Estecio, MR;Davanlou, M;Geng, QR;Fang, Z;Nguyen, M;Pierce, S;Wei, Y;Parmar, S;Cortes, J;Kantarjian, H;Garcia-Manero, G;Expression of PD-L1, PD-L2, PD-1 and CTLA4 in myelodysplastic syndromes is enhanced by treatment with hypomethylating agentsLeukemia24270737from bone marrow specimens using CD34+ MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s protocol. Bone marrow samples from 4 healthy donors were obtained from Allcells (Emeryville, CA). Peripheral blood (PB) samFull Paper
2014Kharabi Masouleh, B;Geng, H;Hurtz, C;Chan, LN;Logan, AC;Chang, MS;Huang, C;Swaminathan, S;Sun, H;Paietta, E;Melnick, AM;Koeffler, P;Müschen, M;Mechanistic rationale for targeting the unfolded protein response in pre-B acute lymphoblastic leukemiaProceedings of the National Academy of Sciences of the United States of America24821775For magnetic bead sorting, peripheral blood or bone marrow mononuclear cells were purchased from AllCells LLC and CD19-enrichment was performed by magnetic bead cell sorting SaveFull Paper
2014Murray, JC;Aldeghaither, D;Wang, S;Nasto, RE;Jablonski, SA;Tang, Y;Weiner, LM;c-Abl modulates tumor cell sensitivity to antibody-dependent cellular cytotoxicityCancer immunology research25300860Frozen primary peripheral blood mononuclear cells (PBMC) from three individual donors (AllCells) were enriched for NK cells (Human NK Cell Enrichment Kit; STEMCELL Technologies SaveFull Paper
2014Wang, Y;Yi, S;Sun, L;Huang, Y;Zhang, M;Charge-selective fractions of naturally occurring nanoparticles as bioactive nanocarriers for cancer therapyActa biomaterialia24952072Splenocytes, derived from C57BL/6 mice, were purchased from the Allcells Company (Emeryville, CA). HEPES, 1,9-dimethyl-methylene blue (DMMB), chondroitin sulfate (CS), Sephadex G75, DEAE-cellulose, and phosphate-buffered saline (PBS) were purchased fFull Paper
2014Fu, Y;Yip, A;Seah, PG;Blasco, F;Shi, PY;Hervé, M;Modulation of inflammation and pathology during dengue virus infection by p38 MAPK inhibitor SB203580Antiviral research25131378Dengue virus (DENV) infection could lead to dengue fever (DF), dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). The disease outcome is controlled by both viral SaveFull Paper
2014Rampal, R;Alkalin, A;Madzo, J;Vasanthakumar, A;Pronier, E;Patel, J;Li, Y;Ahn, J;Abdel-Wahab, O;Shih, A;Lu, C;Ward, PS;Tsai, JJ;Hricik, T;Tosello, V;Tallman, JE;Zhao, X;Daniels, D;Dai, Q;Ciminio, L;Aifantis, I;He, C;Fuks, F;Tallman, MS;Ferrando, A;Nimer, S;Paietta, E;Thompson, CB;Licht, JD;Mason, CE;Godley, LA;Melnick, A;Figueroa, ME;Levine, RL;DNA hydroxymethylation profiling reveals that WT1 mutations result in loss of TET2 function in acute myeloid leukemiaCell reports25482556Eleven human CD34 + bone marrow samples were provided by the Stem Cell and Xenograft Core Facility of the University of Pennsylvania or purchased from AllCells. These studies SaveFull Paper
2014Li, D;Xie, K;Ding, G;Li, J;Chen, K;Li, H;Qian, J;Jiang, C;Fang, J;Tumor resistance to anti-VEGF therapy through up-regulation of VEGF-C expressionCancer letters24333721Human umbilical vein endothelial cells (HUVECs) were obtained from Allcells (Emeryville, , and amphotericin-B (HUVEC-004; Allcells). All cells were authenticated according to the cell SaveFull Paper
2014Li, X;Tong, LJ;Ding, J;Meng, LH;Systematic combination screening reveals synergism between rapamycin and sunitinib against human lung cancerCancer letters24018642The human umbilical vein endothelial cells (HUVECs) were purchased from AllCells and maintained in M199 medium supplemented with 20% heat-inactivated FBS, penicillin (100 IU/ SaveFull Paper
2014Yao, WT;Wu, JF;Yu, GY;Wang, R;Wang, K;Li, LH;Chen, P;Jiang, YN;Cheng, H;Lee, HW;Yu, J;Qi, H;Yu, XJ;Wang, P;Chu, YW;Yang, M;Hua, ZC;Ying, HQ;Hoffman, RM;Jeong, LS;Jia, LJ;Suppression of tumor angiogenesis by targeting the protein neddylation pathwayCell death & disease24525735HUVECs were purchased from Allcells Biotech Co. (Shanghai, China) and cultured in a complete endothelial cell medium (Allcells Biotech Co.) at 37C with 5% CO 2 . SaveFull Paper
2014Li, D;Wei, X;Xie, K;Chen, K;Li, J;Fang, J;A novel decoy receptor fusion protein for FGF-2 potently inhibits tumour growthBritish journal of cancer24874473cells were purchased from AllCells (Emeryville, CA, USA) and cultured in complete HUVEC medium (AllCells) containing 10% FBS and 10% growth factor supplement (AllCells). Caki-1 SaveFull Paper
2014Haselmayer, P;Camps, M;Muzerelle, M;El Bawab, S;Waltzinger, C;Bruns, L;Abla, N;Polokoff, MA;Jond-Necand, C;Gaudet, M;Benoit, A;Bertschy Meier, D;Martin, C;Gretener, D;Lombardi, MS;Grenningloh, R;Ladel, C;Petersen, JS;Gaillard, P;Ji, H;Characterization of Novel PI3Kδ Inhibitors as Potential Therapeutics for SLE and Lupus Nephritis in Pre-Clinical StudiesFrontiers in immunology24904582terial smooth muscle cells and keratinocytes (Lonza, Inc., Allendale, NJ, USA) were as previously described. Positively selected primary normal human CD19+ B cells and CD4+ T-cells were obtained from AllCells (Emeryville, CA, USA). PBMC were isolatedFull Paper
2014Fu, J;Tjandra, M;Becker, C;Bednarczyk, D;Capparelli, M;Elling, R;Hanna, I;Fujimoto, R;Furegati, M;Karur, S;Kasprzyk, T;Knapp, M;Leung, K;Li, X;Lu, P;Mergo, W;Miault, C;Ng, S;Parker, D;Peng, Y;Roggo, S;Rivkin, A;Simmons, RL;Wang, M;Wiedmann, B;Weiss, AH;Xiao, L;Xie, L;Xu, W;Yifru, A;Yang, S;Zhou, B;Sweeney, ZK;Potent nonimmunosuppressive cyclophilin inhibitors with improved pharmaceutical properties and decreased transporter inhibitionJournal of medicinal chemistry25310383PBMCs of two human donors were obtained on the day of the experiment (AllCells, LLC), pelleted in their original 50 mL conical tubes at 1200 rpm for 10 min, and resuspended in 25 SaveFull Paper
2014Gandhi, AK;Mendy, D;Waldman, M;Chen, G;Rychak, E;Miller, K;Gaidarova, S;Ren, Y;Wang, M;Breider, M;Carmel, G;Mahmoudi, A;Jackson, P;Abbasian, M;Cathers, BE;Schafer, PH;Daniel, TO;Lopez-Girona, A;Thakurta, A;Chopra, R;Measuring cereblon as a biomarker of response or resistance to lenalidomide and pomalidomide requires use of standardized reagents and understanding of gene complexityBritish journal of haematology24206017here, H929 lenalidomide-resistant cells and DF15R pomalidomide-resistant cells were taken out of culture with compounds for 5-7 d before use. Select primary CD138+ MM cells were purchased from AllCells (Emeryville, CA, USA). Production of recombinantFull Paper
2014Sully, EK;Malachowa, N;Elmore, BO;Alexander, SM;Femling, JK;Gray, BM;DeLeo, FR;Otto, M;Cheung, AL;Edwards, BS;Sklar, LA;Horswill, AR;Hall, PR;Gresham, HD;Selective chemical inhibition of agr quorum sensing in Staphylococcus aureus promotes host defense with minimal impact on resistancePLoS pathogens24945495Human neutrophils were purchased from AllCells and the source of the neutrophils was Human neutrophils (AllCells) were washed twice in saline to remove EDTA, suspended in RPMI SaveFull Paper
2014Bellayr, IH;Catalano, JG;Lababidi, S;Yang, AX;Lo Surdo, JL;Bauer, SR;Puri, RK;Gene markers of cellular aging in human multipotent stromal cells in cultureStem cell research & therapy24780490Human MSCs from six different donors were purchased from ALLCELLS and Lonza (Table 1). Because these cells were commercially available, no patient consent or approval from the SaveFull Paper
2014Wei, DG;Chiang, V;Fyne, E;Balakrishnan, M;Barnes, T;Graupe, M;Hesselgesser, J;Irrinki, A;Murry, JP;Stepan, G;Stray, KM;Tsai, A;Yu, H;Spindler, J;Kearney, M;Spina, CA;McMahon, D;Lalezari, J;Sloan, D;Mellors, J;Geleziunas, R;Cihlar, T;Histone deacetylase inhibitor romidepsin induces HIV expression in CD4 T cells from patients on suppressive antiretroviral therapy at concentrations achieved by clinical dosingPLoS pathogens24722454ofectamine 2000 (Life Technologies, Grand Island, NY). In vitro model of HIV latency Total peripheral blood mononuclear cells (PBMCs) were obtained from healthy HIV-negative donors by leukapheresis (AllCells, Inc, Emeryville, CA). Naive CD4+ T cellsFull Paper
2014Jajosky, AN;Coad, JE;Vos, JA;Martin, KH;Senft, JR;Wenger, SL;Gibson, LF;RepSox slows decay of CD34+ acute myeloid leukemia cells and decreases T cell immunoglobulin mucin-3 expressionStem cells translational medicine24855276CD34 + cells isolated from the mobilized peripheral blood of healthy donors were purchased from AllCells (Emeryville, CA, http://www.allcells.com). SaveFull Paper
2014Ji, J;Judkowski, VA;Liu, G;Wang, H;Bunying, A;Li, Z;Xu, M;Bender, J;Pinilla, C;Yu, JS;Identification of novel human leukocyte antigen-A*0201-restricted, cytotoxic T lymphocyte epitopes on CD133 for cancer stem cell immunotherapyStem cells translational medicine24375541bone marrow cells were purchased directly from AllCells Inc. (Alameda, CA, http://www. ) cells (CD133+ and CD133‐ fractions; AllCells) from a healthy donor were included as controls. SaveFull Paper
2014Wang, X;Kimbrel, EA;Ijichi, K;Paul, D;Lazorchak, AS;Chu, J;Kouris, NA;Yavanian, GJ;Lu, SJ;Pachter, JS;Crocker, SJ;Lanza, R;Xu, RH;Human ESC-derived MSCs outperform bone marrow MSCs in the treatment of an EAE model of multiple sclerosisStem cell reports25068126BM-MSC lines #1, #2 and #3 were derived from frozen BM mononuclear cells (BMMNC) from 3 independent human donors and BM-MSC lines #7 and #8 were derived from fresh whole bone marrow aspirates from 2 independent donors from AllCells, Inc. BM-MSC lineFull Paper
2014Karjalainen, K;Pasqualini, R;Cortes, JE;Kornblau, SM;Lichtiger, B;O'Brien, S;Kantarjian, HM;Sidman, RL;Arap, W;Koivunen, E;Design, development, and validation of a high-throughput drug-screening assay for targeting of human leukemiaCancer24496871negative against blood-transmittable diseases. These samples were stored at 4°C for 24 hours before use. Heparin was used as an anticoagulant. Pretested whole blood and bone marrow samples also were obtained from commercial sources (Innovative ResearFull Paper
2014Polakowski, N;Terol, M;Hoang, K;Nash, I;Laverdure, S;Gazon, H;Belrose, G;Mesnard, JM;Césaire, R;Péloponèse, JM;Lemasson, I;HBZ stimulates brain-derived neurotrophic factor/TrkB autocrine/paracrine signaling to promote survival of human T-cell leukemia virus type 1-Infected T cellsJournal of virology25210182RNA from healthy donor CD4 T cells was purchased from AllCells. cDNA was synthesized Real-time PCR amplification of cDNAs from cell lines and cDNA purchased from AllCells was SaveFull Paper
2014Lucera, MB;Tilton, CA;Mao, H;Dobrowolski, C;Tabler, CO;Haqqani, AA;Karn, J;Tilton, JC;The histone deacetylase inhibitor vorinostat (SAHA) increases the susceptibility of uninfected CD4+ T cells to HIV by increasing the kinetics and efficiency of postentry viral eventsJournal of virology25008921CD4+ T cells were obtained by leukopheresis from ALLCELLS, LLC. All subjects were 151 Save;CD4 T cells were obtained by leukapheresis from Allcells, LLC. All subjects were healthy control donors negative for hepatitis B virus (HBV), hepatitis C virFull Paper
2014Tabler, CO;Lucera, MB;Haqqani, AA;McDonald, DJ;Migueles, SA;Connors, M;Tilton, JC;CD4+ memory stem cells are infected by HIV-1 in a manner regulated in part by SAMHD1 expressionJournal of virology24554663Samples from healthy donors were deidentified and were obtained by leukapheresis from AllCells, LLC. HIV-infected patients were recruited from the Clinical Research Center, NIH ( SaveFull Paper
2014Chen, YL;Abdul Ghafar, N;Karuna, R;Fu, Y;Lim, SP;Schul, W;Gu, F;Herve, M;Yokohama, F;Wang, G;Cerny, D;Fink, K;Blasco, F;Shi, PY;Activation of peripheral blood mononuclear cells by dengue virus infection depotentiates balapiravirJournal of virology24257621Antiviral assays. Cryopreserved human PBMCs were purchased from AllCells, LLC (Alameda, California). Written consent from the donors was available for all samples. All experiments involving human PBMCs were approved by the Institutional Review BoardFull Paper
2014Raval, AN;Schmuck, EG;Tefera, G;Leitzke, C;Ark, CV;Hei, D;Centanni, JM;de Silva, R;Koch, J;Chappell, RG;Hematti, P;Bilateral administration of autologous CD133+ cells in ambulatory patients with refractory critical limb ischemia: lessons learned from a pilot randomized, double-blind, placebo-controlled trialCytotherapy25239491Purity and viability studies were also performed on mobilized leukapheresis products obtained from healthy normal volunteers (AllCells, Almeda, CA, USA). The dose of G-CSF for SaveFull Paper
2014Hulspas, R;Villa-Komaroff, L;Koksal, E;Etienne, K;Rogers, P;Tuttle, M;Korsgren, O;Sharpe, JC;Berglund, D;Purification of regulatory T cells with the use of a fully enclosed high-speed microfluidic systemCytotherapy25065635kit (Stem Cell Technologies Inc, Vancouver, BC, Canada) from a total of 450 million fresh human peripheral blood mononucleated cells obtained from apheresis leuko paks (AllCells SaveFull Paper
2014Dasmahapatra, G;Patel, H;Friedberg, J;Quayle, SN;Jones, SS;Grant, S;In vitro and in vivo interactions between the HDAC6 inhibitor ricolinostat (ACY1215) and the irreversible proteasome inhibitor carfilzomib in non-Hodgkin lymphoma cellsMolecular cancer therapeutics25239935Interactions between the HDAC6 inhibitor ricolinostat (ACY1215) and the irreversible proteasome inhibitor carfilzomib were examined in non-Hodgkin lymphoma (NHL) models, SaveFull Paper
2014Feng, JY;Cheng, G;Perry, J;Barauskas, O;Xu, Y;Fenaux, M;Eng, S;Tirunagari, N;Peng, B;Yu, M;Tian, Y;Lee, YJ;Stepan, G;Lagpacan, LL;Jin, D;Hung, M;Ku, KS;Han, B;Kitrinos, K;Perron, M;Birkus, G;Wong, KA;Zhong, W;Kim, CU;Carey, A;Cho, A;Ray, AS;Inhibition of hepatitis C virus replication by GS-6620, a potent C-nucleoside monophosphate prodrugAntimicrobial agents and chemotherapy24419349Human primary bone marrow (BM) light-density cells from three different lots were obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD), and the cytotoxicity studies were SaveFull Paper
2014Yao, H;Miura, Y;Yoshioka, S;Miura, M;Hayashi, Y;Tamura, A;Iwasa, M;Sato, A;Hishita, T;Higashi, Y;Kaneko, H;Ashihara, E;Ichinohe, T;Hirai, H;Maekawa, T;Parathyroid hormone enhances hematopoietic expansion via upregulation of cadherin-11 in bone marrow mesenchymal stromal cellsStem cells (Dayton, Ohio)24648356Parathyroid hormone (PTH) stimulates hematopoiesis in mouse models. The involvement of osteoblasts in this process has been well investigated; however, the effects of PTH on SaveFull Paper
2014Pandya, J;Ortiz, L;Ling, C;Rivers, AE;Aslanidi, G;Rationally designed capsid and transgene cassette of AAV6 vectors for dendritic cell-based cancer immunotherapyImmunology and cell biology24217810Leukapheresis-derived peripheral blood mononuclear cells (AllCells, Alameda, CA, USA) were purified on Ficoll-Paque (GE Healthcare, Piscataway, NJ, USA). Semi-adherent cell fractions were incubated in serum-free X-VIVO medium (Lonza, Allendale, NJ, UFull Paper
2014Yoshioka, S;Miura, Y;Yao, H;Satake, S;Hayashi, Y;Tamura, A;Hishita, T;Ichinohe, T;Hirai, H;Takaor-Kondo, A;Maekawa, T;CCAAT/enhancer-binding protein β expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesisStem cells (Dayton, Ohio)24115241The transcription factor CCAAT/enhancer-binding protein β (C/EBPβ) regulates the differentiation of a variety of cell types. Here, the role of C/EBPβ expressed by bone marrow SaveFull Paper
2014Brunckhorst, MK;Xu, Y;Lu, R;Yu, Q;Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironmentThe American journal of pathology25043619Despite decades of research, the survival rate of ovarian cancer patients is largely unchanged. Current chemotherapeutic drugs are effective only transiently because patients with SaveFull Paper
2014Bowers, PM;Verdino, P;Wang, Z;da Silva Correia, J;Chhoa, M;Macondray, G;Do, M;Neben, TY;Horlick, RA;Stanfield, RL;Wilson, IA;King, DJ;Nucleotide insertions and deletions complement point mutations to massively expand the diversity created by somatic hypermutation of antibodiesThe Journal of biological chemistry25320089Sequencing and Preparation of PBMC cDNA RNA from peripheral blood lymphocytes (PBMCs) from a total of 68 healthy donors was purchased from two sources as follows: seven donors from AllCells, Inc. (Alameda, CA), and 61 donors from HemaCare Corp. (SanFull Paper
2014Edwards, W;Fung-Leung, WP;Huang, C;Chi, E;Wu, N;Liu, Y;Maher, MP;Bonesteel, R;Connor, J;Fellows, R;Garcia, E;Lee, J;Lu, L;Ngo, K;Scott, B;Zhou, H;Swanson, RV;Wickenden, AD;Targeting the ion channel Kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-lifeThe Journal of biological chemistry24939846Human PBMCs were obtained from AllCells LLC or purified from healthy donor blood by Ficoll-Paque (GE Healthcare) density centrifugation. Purified PBMCs were plated in 96-well SaveFull Paper
2014Stuible, M;Moraitis, A;Fortin, A;Saragosa, S;Kalbakji, A;Filion, M;Tremblay, GB;Mechanism and function of monoclonal antibodies targeting siglec-15 for therapeutic inhibition of osteoclastic bone resorptionThe Journal of biological chemistry24446437HOPs (CD14 + peripheral blood mononuclear cells) were isolated from normal human peripheral blood mononuclear cells (AllCells, Emeryville, CA) using CD14 microbeads and MS SaveFull Paper
2014Collins, E;Gu, F;Qi, M;Molano, I;Ruiz, P;Sun, L;Gilkeson, GS;Differential efficacy of human mesenchymal stem cells based on source of originJournal of immunology (Baltimore, Md. : 1950)25274529Human BM MSCs were derived from fresh whole BM of healthy donors (purchased from ALLCELLS) and four lupus patients. Lupus patients consisted of four African Americans (three SaveFull Paper
2014Lan, J;Yang, K;Byrd, D;Hu, N;Amet, T;Shepherd, N;Desai, M;Gao, J;Gupta, S;Sun, Y;Yu, Q;Provirus activation plus CD59 blockage triggers antibody-dependent complement-mediated lysis of latently HIV-1-infected cellsJournal of immunology (Baltimore, Md. : 1950)25149467Briefly, leukapheresis products from 3 HIV-1-negative blood donors were purchased from the ALLCells (Emeryville, CA) for isolation of primary CD4+ T cells using the negative selection RosetteSep reagents (StemCell Technologies, Vancouver, BC, Canada)Full Paper
2014Balan, S;Ollion, V;Colletti, N;Chelbi, R;Montanana-Sanchis, F;Liu, H;Vu Manh, TP;Sanchez, C;Savoret, J;Perrot, I;Doffin, AC;Fossum, E;Bechlian, D;Chabannon, C;Bogen, B;Asselin-Paturel, C;Shaw, M;Soos, T;Caux, C;Valladeau-Guilemond, J;Dalod, M;Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cellsJournal of immunology (Baltimore, Md. : 1950)25009205CD34 + cells from adult donor bone marrow or mobilized peripheral blood were purchased from properly licensed commercial companies (AllCells and StemCells). SaveFull Paper
2014Kajiwara, Y;Schiff, T;Voloudakis, G;Gama Sosa, MA;Elder, G;Bozdagi, O;Buxbaum, JD;A critical role for human caspase-4 in endotoxin sensitivityJournal of immunology (Baltimore, Md. : 1950)24879791Human monocyte-derived macrophages were purchased from AllCells (PB-MDM-001F; Alameda, CA) and cultured in RPMI 1640 supplemented with 10% FBS/penicillin/streptomycin and 10 ng/ml human M-CSF (R&D Systems). BMDM cultures were prepared according to prFull Paper
2014Kohn, LA;Seet, CS;Scholes, J;Codrea, F;Chan, R;Zaidi-Merchant, S;Zhu, Y;De Oliveira, S;Kapoor, N;Shah, A;Abdel-Azim, H;Kohn, DB;Crooks, GM;Human lymphoid development in the absence of common γ-chain receptor signalingJournal of immunology (Baltimore, Md. : 1950)24771849Normal human adult BM was obtained from healthy donors as anonymous waste materials isolated from filters after BM harvest and from commercial sources (AllCells, Alameda, CA). SaveFull Paper
2014Inage, E;Kasakura, K;Yashiro, T;Suzuki, R;Baba, Y;Nakano, N;Hara, M;Tanabe, A;Oboki, K;Matsumoto, K;Saito, H;Niyonsaba, F;Ohtsuka, Y;Ogawa, H;Okumura, K;Shimizu, T;Nishiyama, C;Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2Journal of immunology (Baltimore, Md. : 1950)24639354Human primary mast cells were generated from peripheral blood CD34+ cells (Frozen Mobilized Peripheral Blood CD34+ cells, mPB015F; ALLCELLS, Emeryville, CA) as progenitors based on the previously established method (13). In three independent experimeFull Paper
2014Fukahori, H;Chida, N;Maeda, M;Tasaki, M;Kawashima, T;Matsuoka, H;Suzuki, K;Ishikawa, T;Tanaka, A;Higashi, Y;Effect of AS2521780, a novel PKCθ selective inhibitor, on T cell-mediated immunityEuropean journal of pharmacology25445039T cell-mediated immunity is central to the pathogenesis of autoimmune diseases, and is a target in the development of alternative therapeutic strategies with reduced adverse effects on SaveFull Paper
2014Kota, DJ;DiCarlo, B;Hetz, RA;Smith, P;Cox, CS;Olson, SD;Differential MSC activation leads to distinct mononuclear leukocyte binding mechanismsScientific reports24691433For Supplemental Figure 1, we also isolated MSCs from commercially available fresh bone marrow aspirates (AllCells, Emeryville, CA) according to the protocols previously established SaveFull Paper
2014Lee, JS;Kim, YR;Park, JM;Ha, SJ;Kim, YE;Baek, NI;Hong, EK;Mulberry fruit extract protects pancreatic β-cells against hydrogen peroxide-induced apoptosis via antioxidative activityMolecules (Basel, Switzerland)24972274and then 0.7 mM hydrogen peroxide was added to allcells for 4 h, with the exception of the h and then 0.7 mM hydrogen peroxide was added to allcells for 4 h, with the exception of the SaveFull Paper
2014Langevin, SM;Houseman, EA;Accomando, WP;Koestler, DC;Christensen, BC;Nelson, HH;Karagas, MR;Marsit, CJ;Wiencke, JK;Kelsey, KT;Leukocyte-adjusted epigenome-wide association studies of blood from solid tumor patientsEpigenetics24671036Briefly, sorted non-pathological human peripheral blood leukocytes (n = 47) were previously obtained from different anonymous individuals through AllCells, LLC. Leukocytes were SaveFull Paper
2014Xiao, W;Wang, J;Li, H;Xia, D;Yu, G;Yao, W;Yang, Y;Xiao, H;Lang, B;Ma, X;Guo, X;Guan, W;Xu, H;Liu, J;Zhang, X;Ye, Z;Fibulin-1 is epigenetically down-regulated and related with bladder cancer recurrenceBMC cancer25234557O2 maintained at 37°C. SV-HUC-1 was also purchased from ATCC and maintained in DMEM/F-12 medium. Primary human umbilical vein endothelial cell (HUVEC) and Endothelial Cell Medium were purchased from ALLCELLS, LLC (Shanghai, China). Plasmid transfectiFull Paper
2014Thorarensen, A;Banker, ME;Fensome, A;Telliez, JB;Juba, B;Vincent, F;Czerwinski, RM;Casimiro-Garcia, A;ATP-mediated kinome selectivity: the missing link in understanding the contribution of individual JAK Kinase isoforms to cellular signalingACS chemical biology24814050Cryopreserved human PBMCs (no. PB005F), which were used in the IL-2, IL-10, IL-15, and IFNα assays, were purchased from Allcells (Emeryville, CA). Frozen PBMCs were thawed in a water bath (37 °C) and washed once with once with 5% FBS/RPMI-1640Full Paper
2014Womble, TA;Green, S;Shahaduzzaman, M;Grieco, J;Sanberg, PR;Pennypacker, KR;Willing, AE;Monocytes are essential for the neuroprotective effect of human cord blood cells following middle cerebral artery occlusion in ratMolecular and cellular neurosciences24472845The cells (either HUCB or HPB MNC, obtained commercially from AllCells, LLC), were thawed rapidly at 37 C and transferred slowly into 10 ml of 10 mM PBS. The cells were then SaveFull Paper
2014Fraser, P;Macdonald, G;Mullineux, A;Regional Monetary Policy: An Australian PerspectiveRegional StudiesThis is a feature which has been under discussion and analysis for some time (for example, Tremosa-Ballcells and Pons-Novell, 2001; Mojon and Peersman, 2001; Aksoy et al., 2002; Clausen and Wohltmann, 2005; and more recently, Dow et al., 2012; and alFull Paper
2014Liu, X;Ou, YC;Zhang, J;Ahene, A;Clark, D;Hsieh, SC;Cooper, M;Ji, C;Pharmacokinetics and pharmacodynamics of CD4-anchoring bi-functional fusion inhibitor in monkeysPharmaceutical research24065594BD Biosciences (La Jolla, CA). Human PBMCs from healthy donors were purchased from AllCells (cat # PB001, Emeryville, CA) and cultured in complete medium (RPMI 1640, 10% FBS, 1% P/S, 1% L-Glutamine, 1% non-essential amino acid, 1% sodium pyruvate, 1%Full Paper
2014Wolstein, O;Boyd, M;Millington, M;Impey, H;Boyer, J;Howe, A;Delebecque, F;Cornetta, K;Rothe, M;Baum, C;Nicolson, T;Koldej, R;Zhang, J;Keech, N;Camba Colón, J;Breton, L;Bartlett, J;An, DS;Chen, IS;Burke, B;Symonds, GP;Preclinical safety and efficacy of an anti-HIV-1 lentiviral vector containing a short hairpin RNA to CCR5 and the C46 fusion inhibitorMolecular therapy. Methods & clinical development26015947ced overnight on RetroNectin (TaKaRa, Japan, #T100B) coated plates at various MOI, as indicated in the experimental methodology. CD34+ cells Primary human CD34+ HSPC were purchased commercially from AllCells; cells were directly isolated from granulFull Paper
2014Palasuk, J;Kamocki, K;Hippenmeyer, L;Platt, JA;Spolnik, KJ;Gregory, RL;Bottino, MC;Bimix antimicrobial scaffolds for regenerative endodonticsJournal of endodontics25201643Introduction Eliminating and/or inhibiting bacterial growth within the root canal system has been shown to play a key role in the regenerative outcome. The aim of this study was to SaveFull Paper
2014Zhang, HT;Wang, L;Ai, J;Chen, Y;He, CX;Ji, YC;Huang, M;Yang, JY;Zhang, A;Ding, J;Geng, MY;SOMG-833, a novel selective c-MET inhibitor, blocks c-MET-dependent neoplastic effects and exerts antitumor activityThe Journal of pharmacology and experimental therapeutics24741075HUVEC cells (passage 3) were first serum starved in complete medium (AllCells) for 24 hours and treated by SOMG-833 for 72 hours in media containing 3% bovine serum albumin and SaveFull Paper
2014Kimbrel, EA;Kouris, NA;Yavanian, GJ;Chu, J;Qin, Y;Chan, A;Singh, RP;McCurdy, D;Gordon, L;Levinson, RD;Lanza, R;Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic propertiesStem cells and development24650034from fresh BM aspirates (AllCells LLC, Alameda, CA) using Histopaque 1077/Accuspin tubes (Sigma-Aldrich) or from frozen human BM mononuclear cells (AllCells LLC) from both male SaveFull Paper
2014Iwata, M;Sandstrom, RS;Delrow, JJ;Stamatoyannopoulos, JA;Torok-Storb, B;Functionally and phenotypically distinct subpopulations of marrow stromal cells are fibroblast in origin and induce different fates in peripheral blood monocytesStem cells and development24131213The cells were cultured in mesenchymal stromal cell (MSC) basal media with MSC stimulatory supplement according to the manufacturer's protocol (ALLCELLS), and then expanded in SaveFull Paper
2014Johnston, JM;Denning, G;Moot, R;Whitehead, D;Shields, J;Le Doux, JM;Doering, CB;Spencer, HT;High-throughput screening identifies compounds that enhance lentiviral transductionGene therapy25231175Fresh CD34+ cells were purchased from AllCells (Emeryville, CA, USA). Before arrival, CD34+ cells were mobilized from human bone marrow and isolated from the peripheral blood of three individual subjects. Flow cytometry was used to assess the purityFull Paper
2014Kamata, S;Miyagawa, S;Fukushima, S;Nakatani, S;Kawamoto, A;Saito, A;Harada, A;Shimizu, T;Daimon, T;Okano, T;Asahara, T;Sawa, Y;Improvement of cardiac stem cell sheet therapy for chronic ischemic injury by adding endothelial progenitor cell transplantation: analysis of layer-specific regional cardiac functionCell transplantation23562134Each CSC sheet was approximately 42 mm in diameter and 100 μm thick. Granulocyte colony-stimulating factor-mobilized EPCs of human origin (AllCells, MPB-017F; Emeryville, CA, USA) were labeled with 2 μM DiI-blue in vitro (Molecular Probes) (33). TheFull Paper
2014Xiao, H;Li, H;Yu, G;Xiao, W;Hu, J;Tang, K;Zeng, J;He, W;Zeng, G;Ye, Z;Xu, H;MicroRNA-10b promotes migration and invasion through KLF4 and HOXD10 in human bladder cancerOncology reports24573354SV-HUC-1 was also purchased from ATCC and maintained in DMEM/F-12 medium. SCaBER was purchased from AllCells, LLC (Shanghai, China) in MEM medium. Paired samples of primary BC, and lymph node metastatic tissues were obtained from patients who had undFull Paper
2014Czubak, P;Bojarska-Junak, A;Tabarkiewicz, J;Putowski, L;A modified method of insulin producing cells' generation from bone marrow-derived mesenchymal stem cellsJournal of diabetes research25405207Human bone marrow stromal cells (MSCs) were purchased from a commercial source (AllCells; catalog number MSC-001F). This cell line was derived from one, nondiabetic Caucasian SaveFull Paper
2014Gupta, MS;Nicoll, SB;Functional nucleus pulposus-like matrix assembly by human mesenchymal stromal cells is directed by macromer concentration in photocrosslinked carboxymethylcellulose hydrogelsCell and tissue research25092545Human MSCs were purified from bone marrow (AllCells, Emeryville, CA, USA) using enriched density centrifugation as previously described (Gupta et al. 2011; Chen et al. 2013). Briefly, a Human MSC Enrichment Cocktail (StemCell Technologies, Vancouver,Full Paper
2014Pavlides, S;Gutierrez-Pajares, JL;Iturrieta, J;Lisanti, MP;Frank, PG;Endothelial caveolin-1 plays a major role in the development of atherosclerosisCell and tissue research24390341Human umbilical vein endothelial cells (HUVEC) were obtained from AllCells (AllCells, LLC, Emeryville, CA, USA). HUVEC were cultured in M199 media (Life Technologies, Grand Island, NY, USA) containing 10 % FBS (Life Technologies, Grand Island, NY, USFull Paper
2014Kiang, JG;Garrison, BR;Smith, JT;Fukumoto, R;Ciprofloxacin as a potential radio-sensitizer to tumor cells and a radio-protectant for normal cells: differential effects on γ-H2AX formation, p53 phosphorylation, Bcl-2 production, and cell deathMolecular and cellular biochemistry24802382Human B lymphoblastoid cell line TK6 (p53+/+) and human NH32 (p53−/− of TK6 cells) were generous gifts from Dr. James Mitchell. Human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Emeryville, CA). Cells were grown in RPMI 1Full Paper
2014Dong, B;Zhou, H;Han, C;Yao, J;Xu, L;Zhang, M;Fu, Y;Xia, Q;Ischemia/reperfusion-induced CHOP expression promotes apoptosis and impairs renal function recovery: the role of acidosis and GPR4PloS one25343248Human umbilical vein endothelial cells (HUVECs), as well as supplier's recommended medium and supplement were purchased from ALLCELLS (Emeryville, California, USA). Human SaveFull Paper
2014Iwata, M;Torok-Storb, B;Wayner, EA;Carter, WG;CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine productionPloS one25275584The cells were cultured in MSC Basal media with MSC stimulatory supplement according to the manufacturer's protocol (ALLCELLS, Emeryville, CA). The cells were passaged until the SaveFull Paper
2014Ayala-Lujan, JL;Vijayakumar, V;Gong, M;Smith, R;Santiago, AE;Ruiz-Perez, F;Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytesPloS one25251283Fresh human blood to isolate primary cells were purchased from Allcells LLC. (Alameda, Fresh heparinized blood from healthy human donors purchased from AllCells LLC. (Alameda, SaveFull Paper
2014Kang, Y;Hodges, A;Ong, E;Roberts, W;Piermarocchi, C;Paternostro, G;Identification of drug combinations containing imatinib for treatment of BCR-ABL+ leukemiasPloS one25029499, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, USA), 100 U/ml penicillin, 100 µg/ml streptomycin (Hyclone, USA) at 37°C in 5% CO2. CD34+ hematopoietic stem cells were purchased from Allcells and maintained in StemSpan SFEM (STEMCELL TFull Paper
2014Lewis, ND;Patnaude, LA;Pelletier, J;Souza, DJ;Lukas, SM;King, FJ;Hill, JD;Stefanopoulos, DE;Ryan, K;Desai, S;Skow, D;Kauschke, SG;Broermann, A;Kuzmich, D;Harcken, C;Hickey, ER;Modis, LK;A GPBAR1 (TGR5) small molecule agonist shows specific inhibitory effects on myeloid cell activation in vitro and reduces experimental autoimmune encephalitis (EAE) in vivoPloS one24967665Human peripheral blood mononuclear cells isolated with leukapheresis were purchased from AllCells (Alameda, CA), frozen in 80% FBS, 10% RPMI and 10% DMSO and stored in SaveFull Paper
2014Simeonov, KP;Uppal, H;Direct reprogramming of human fibroblasts to hepatocyte-like cells by synthetic modified mRNAsPloS one24963715ield of direct reprogramming can overcome its retroviral reliance and be translated to its ultimate applications in regenerative medicine. Materials and Methods Cell Culture CD34+ bone marrow cells (AllCells, Emeryville, CA) were received fresh andFull Paper
2014Hong, KU;Guo, Y;Li, QH;Cao, P;Al-Maqtari, T;Vajravelu, BN;Du, J;Book, MJ;Zhu, X;Nong, Y;Bhatnagar, A;Bolli, R;c-kit+ Cardiac stem cells alleviate post-myocardial infarction left ventricular dysfunction despite poor engraftment and negligible retention in the recipient heartPloS one24806457m (A LV) which included the infarcted and border zones, posterior LV wall (nonischemic region; P LV), and atria+right ventricle. Female human peripheral mononuclear cells (hPBMCs) were purchased from AllCells, LLC (Emeryville, CA). Aliquots of 105 hPFull Paper
2014Gañán-Gómez, I;Wei, Y;Yang, H;Pierce, S;Bueso-Ramos, C;Calin, G;Boyano-Adánez, Mdel C;García-Manero, G;Overexpression of miR-125a in myelodysplastic syndrome CD34+ cells modulates NF-κB activation and enhances erythroid differentiation arrestPloS one24690917Human bone marrow cells from 6 healthy individuals were obtained from AllCells (Emeryville, CA). Isolation of CD34 + cells was performed using MicroBead Kit (Miltenyi, Bergisch SaveFull Paper
2014Chyu, KY;Lio, WM;Dimayuga, PC;Zhou, J;Zhao, X;Yano, J;Trinidad, P;Honjo, T;Cercek, B;Shah, PK;Cholesterol lowering modulates T cell function in vivo and in vitroPloS one24647529ential batch-to-batch differences in the biochemical contents in the FBS which may affect the experimental outcomes. The same experiment was performed using human peripheral blood mononuclear cells (AllCells, Emeryville, CA). T cells were isolated fFull Paper
2014Chen, J;Li, C;Wang, S;Periodic heat shock accelerated the chondrogenic differentiation of human mesenchymal stem cells in pellet culturePloS one24632670nd Methods All reagents and chemicals without manufacture labels were purchased from Sigma-Aldrich (St Louis, MO). hMSC Isolation and Characterization Human MSCs were isolated from bone marrow (BM) (AllCells LLC, Berkeley, CA) as described previouslFull Paper
2014Wang, J;Guo, X;Lui, M;Chu, PJ;Yoo, J;Chang, M;Yen, Y;Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitroPloS one24465489This study was conducted using data obtained from 70 fresh hPB and 30 fresh hBM samples purchased from AllCells, LLC. A list of the reagents and antibodies used in this study is SaveFull Paper
2014Beckwith, R;Worden, E;Estrin, E;Martin, A;Reconstitution of the 26S Proteasome Reveals Functional Asymmetries in its Heterohexameric AAA+ UnfoldaseBiophysical JournalProteins are complex and versatile macromolecules that are integral components of allcells. Among other things, proteins are responsible for structural organization, signalingand SaveFull Paper
2014Aikawa, N;Kunisato, A;Nagao, K;Kusaka, H;Takaba, K;Ohgami, K;Detection of thalidomide embryotoxicity by in vitro embryotoxicity testing based on human iPS cellsJournal of pharmacological sciences24451995The hiPSCs were derived from human bone marrow mononuclear cells which were purchased from AllCells (Emeryville, CA, USA) (11). Human dermal fibroblasts isolated from adult SaveFull Paper
2014Chen, JK;Li, ZP;Liu, YZ;Zhao, T;Zhao, XB;Ni, M;Jiang, GJ;Shen, FM;Activation of alpha 7 nicotinic acetylcholine receptor protects mice from radiation-induced intestinal injury and mortalityRadiation research24828112Radiation-induced gastrointestinal syndrome occurs when the body is exposed to a high dose of radiation. Currently, safe and effective radioprotectants are not available. Apoptosis SaveFull Paper
2014Lee, M;Jeong, SY;Ha, J;Kim, M;Jin, HJ;Kwon, SJ;Chang, JW;Choi, SJ;Oh, W;Yang, YS;Kim, JS;Jeon, HB;Low immunogenicity of allogeneic human umbilical cord blood-derived mesenchymal stem cells in vitro and in vivoBiochemical and biophysical research communications24657442Abstract Evaluation of the immunogenicity of human mesenchymal stem cells (MSCs) in an allogeneic setting during therapy has been hampered by lack of suitable models due to SaveFull Paper
2014Shima, T;Miyamoto, T;Kikushige, Y;Yuda, J;Tochigi, T;Yoshimoto, G;Kato, K;Takenaka, K;Iwasaki, H;Mizuno, S;Goto, N;Akashi, K;The ordered acquisition of Class II and Class I mutations directs formation of human t(8;21) acute myelogenous leukemia stem cellExperimental hematology25101977Human marrow was purchased from AllCells (Emeryville, CA). Informed consent was obtained from all patients. The Institutional Review Board of Kyushu University Hospital (Fukuoka, SaveFull Paper
2014Iancu-Rubin, C;Mosoyan, G;Glenn, K;Gordon, RE;Nichols, GL;Hoffman, R;Activation of p53 by the MDM2 inhibitor RG7112 impairs thrombopoiesisExperimental hematology24309210Human MK were generated in a two-step liquid culture system from bone marrow (BM)-derived CD34+ cells (AllCells, Emeryville, CA, USA). Cells were suspended in IMDM-based MK differentiation medium [6] for 7 days then allowed to mature for 7-9 additionFull Paper
2014Kornblau, SM;Cohen, AC;Soper, D;Huang, YW;Cesano, A;Age-related changes of healthy bone marrow cell signaling in response to growth factors provide insight into low risk MDSCytometry. Part B, Clinical cytometry24106013patients aged 54 to 82 years undergoing planned orthopedic procedures at Williamson Medical Center in Tennessee. The second (“younger”) healthy sample set consisted of 6 cryopreserved BMMC obtained from donors aged 23 to 43 years, purchased from a coFull Paper
2014Barrionuevo, M;Society of Toxicology (SOT) 5st Annual Meeting and ToxExpo, San Francisco, CA, USA, March 11-15, 2012Drugs of the Future283 on human myeloid, erythroid and mesenchymal lineages for its potential to protect marrow progenitor cells from radiation damage was discussed by Emer Clarke from ReachBio. SaveFull Paper
2014Fu, Y;Chen, YL;Herve, M;Gu, F;Shi, PY;Blasco, F;Development of a FACS-based assay for evaluating antiviral potency of compound in dengue infected peripheral blood mononuclear cellsJournal of virological methods24140514Isolated cryopreserved human PBMCs were obtained from AllCells, LLC. The PBMCs were obtained with informed consent and experiments involving PBMCs were approved by SaveFull Paper
2014Tilton, CA;Tabler, CO;Lucera, MB;Marek, SL;Haqqani, AA;Tilton, JC;A combination HIV reporter virus system for measuring post-entry event efficiency and viral outcome in primary CD4+ T cell subsetsJournal of virological methods24025341Peripheral blood mononuclear cells (PBMCs) and CD4+ T cells were obtained from leukopheresis from ALLCELLS, LLC on five healthy donors. All subjects for this study were de- SaveFull Paper
2014Shiheido, H;Kitagori, K;Sasaki, C;Kobayashi, S;Aoyama, T;Urata, K;Oku, T;Hirayama, Y;Yoshitomi, H;Hikida, M;Yoshifuji, H;Mimori, T;Watanabe, T;Shimizu, J;Human T cells expressing BEND3 on their surface represent a novel subpopulation that preferentially produces IL-6 and IL-8Immunity, inflammation and disease25400923, #16-15 Abs, and 7AAD, and BEND3+ or BEND3− subpopulations in CD4+ or CD8+ T cells were sorted using a flow cytometer (FACSAria, BD Biosciences, San Jose, CA). Cord blood cells were purchased from ALLCELLS, and Jurkat [25] cells were from the AmericFull Paper
2014Cheng, ZJ;Garvin, D;Paguio, A;Moravec, R;Engel, L;Fan, F;Surowy, T;Development of a robust reporter-based ADCC assay with frozen, thaw-and-use cells to measure Fc effector function of therapeutic antibodiesJournal of immunological methods25086226PBMCs from different donors were from AllCells. PNGase F, Magne™ Protein G Beads, Bio-Glo™ Luciferase Assay System and CytoTox 96® Non-Radioactive Cytotoxicity Assay were from PromegaFull Paper
2014Furuya, T;Sá, JM;Chitnis, CE;Wellems, TE;Stedman, TT;Reticulocytes from cryopreserved erythroblasts support Plasmodium vivax infection in vitroParasitology international24291603Plasmodium vivax is the most widely distributed human malaria parasite. Despite its importance, both clinical research and basic research have been hampered by lack of a convenient SaveFull Paper
2014Meissner, TB;Mandal, PK;Ferreira, LM;Rossi, DJ;Cowan, CA;Genome editing for human gene therapyMethods in enzymology25398345CD34 + cells from G-CSF mobilized peripheral blood were purchased from AllCells. CD34 + cells were cotransfected with CRISPR/Cas9 expression plasmids using Amaxa SaveFull Paper
2014Wilson, EM;Bial, J;Tarlow, B;Bial, G;Jensen, B;Greiner, DL;Brehm, MA;Grompe, M;Extensive double humanization of both liver and hematopoiesis in FRGN miceStem cell research25310256rich Chemicals; non-platable human hepatocytes (> 70% viable by trypan blue staining) were purchased from BioreclamationIVT (catalog# F00995); human frozen bone marrow CD34+ cells were purchased from AllCells (Catalog# ABM017F); HCM media was purchasFull Paper
2014Yourek, G;Xin, X;Reilly, GC;Mao, JJ;Infiltration of mesenchymal stem cells into PEGDA hydrogelBio-medical materials and engineering25201394Human MSCs were isolated from commercially available whole bone marrow (AllCells, Berkeley, CA) using "RosetteSep " (StemCell Technologies; Vancouver, BC) according to the SaveFull Paper
2014Chang, TP;Vancurova, I;Bcl3 regulates pro-survival and pro-inflammatory gene expression in cutaneous T-cell lymphomaBiochimica et biophysica acta25089799The advanced stages of cutaneous T cell lymphoma (CTCL) are characterized not only by decreased levels of pro-inflammatory cytokines, resulting in high susceptibility to infections, SaveFull Paper
2014Li, J;Sun, W;Subrahmanyam, PB;Page, C;Younger, KM;Tiper, IV;Frieman, M;Kimball, AS;Webb, TJ;NKT Cell Responses to B Cell LymphomaMedical sciences (Basel, Switzerland)24955247tained from commercial vendors. Specifically, buffy coats were purchased from Biological Specialty Corporation and peripheral blood from two different, newly diagnosed MCL patients was purchased from AllCells, LLC (Alameda, CA, USA). PBMCs were isolaFull Paper
2014Gitschier, HJ;Bergeron, AB;Randle, DH;Label-free cell-based dynamic mass redistribution assaysCurrent protocols in chemical biology24652622Human umbilical vein endothelial cells (Corning, cat. no. 354151) Peripheral blood mononuclear cells (AllCells, cat. no. PB006F) Appropriate cell culture/differentiation medium with supplements, e.g.: IMDM, KnockOut DMEM/F-12 with Stempro supplement,Full Paper
2014Vij, R;Mazumder, A;Klinger, M;O'Dea, D;Paasch, J;Martin, T;Weng, L;Park, J;Fiala, M;Faham, M;Wolf, J;Deep sequencing reveals myeloma cells in peripheral blood in majority of multiple myeloma patientsClinical lymphoma, myeloma & leukemia24629890The remaining 13 paired bone marrow and peripheral blood samples were purchased from a commercial source (AllCells, Emeryville, CA). SaveFull Paper
2014DeClerck, Y;Environment-Mediated Drug Resistance in NeuroblastomaU.S. Army Medical Research Material CommandHuman BMMSC were purchased from AllCells LLC. Monocytes of normal healthy donors were obtained from peripheral blood and separated by Ficoll density gradient centrifugation SaveFull Paper
2014Kornblau, S;The Use of “Omics” to Guide the Selection of Targeted TherapyTargeted Therapy of Acute Myeloid LeukemiaIf the normal HSC frequency is 1:100,000, then multiply this by 10. For our protein work, we have therefore used a CD34+ cell from a normal bone marrow as our standard comparator (we have successfully used CD34+ cells from AllCells for our RPPA work)Full Paper
2014허운학, ;Concurrent treatment with capecitabine and radiosurgery in the mouse glioblastoma modelThesisAllCells were cultured for two to three passages in Dulbecco's modified Eagle's medium (WelGENE, Daegu, Korea) containing 10% fetal bovine serum and 1% antibiotic-antimycotic SaveFull Paper
2014Zaidi-Merchant, SS;The role of let-7c/miR-125b/miR-99a in the hematopoietic development in the human bone marrowThesisNormal human bone marrow samples were obtained from healthy donors via the UCLA Pathology Tissue Core, Cincinnati Children’s Hospital, or ALLCELLS according to guidelines approved by UCLA Institutional Review Board. Fresh BM samples were Ficoll-PaqueFull Paper
2014Holm, HH;A CUDA Back-End for the Equelle Compiler.ThesisThe function Gradient takes a Collection Of Scalar On AllCells() as input and compute the discrete If u is a variable defined as Collection Of Scalar On AllCells() then the following line is SaveFull Paper
2014Oien, N;Erythrocyte Carriers: Tool Development for Erythrocyte Mediated Diagnostic and Drug DeliveryThesisPeripheral erythrocytes were purchased from AllCells. Dialysis cassettes were purchased Peripheral red blood cells were purchased from AllCells. 8-(4-chlorophenylthio) adenosine-3′ SaveFull Paper
2014Nguyen, L;LONG WAVELENGTH FLUORESCENT REPORTERS FOR PROTEIN KINASES AND DESIGN OF LIGHT-ACTIVATABLE BIOAGENTSThesisH89 and KT5720 were purchased from Sigma-Aldrich and Calbiochem (EMD), respectively. Peripheral red blood cells were purchased from AllCells. 8-(4-chlorophenylthio) adenosine-3′,5′-cyclic monophosphorothioate (CPT-cAMP), ATP, and cAMP were purchasedFull Paper
2014Rathbone, E;Inhibitory and Immunomodulatory Properties of Human Choroid Plexus Epithelial CellsThesisPooled BMSC, ADSC (both Lonza, Basel, Switzerland), DPSC (AllCells L.L.C., Alameda, CA, USA) and AMSC were cultured in high glucose Dulbecco’s Modified Eagle Medium (HG-DMEM; Biosera, Boussens, France) supplemented with 10% foetal bovine serum (FBS),Full Paper
2014Suganyaa, MV;Deepa, MKRS;Vinothini, MG;Operating the SRAM Cell Architecture with the High Efficient and Reduced Memory Sizeijaist.comIt has a multiplicative effect; allcells in the array are drawing this power. The CMOS 8T cellrequires about 60 times more static power than SRAM cells inthis study. The SRAM 8T cell SaveFull Paper
2014Yu, Y;Energy prediction of electric floor radiation systems using a new integrated modeling approachASHRAE TransactionsIn this figure, PMV Onezone and PPD Onezone represent the PMV and PPD values when the one-zone (multi-zone) model is applied and PMV Allcells and PPD Allcells represent the SaveFull Paper
2014Xiao, F;Genomic study of familial breast cancer-genomic instability, classification and predispositionThesisThe cells for the study were purchased from AllCells LLC (http://www.allcells.com/, Emeryville, California), which supplies human blood cells from donors for research and the company has its full IRB system (Biomed IRB). The signed written consent foFull Paper
2014Perignon, MC;A Rolling Stone Gathers No Moss: Modeling the Geomorphic Impacts of Floods in the Southern Rocky Mountain Region, USAThesis(Top, right) Non-local survival function for sediment entering the system at the mouth of the fan, for allcells with ow depth greater than 1 cm. (Bottom, right) Non-local probability of SaveFull Paper
2014Suwannatat, P;A Visual Analysis Toolkit for Microscopic Image Mosaics of Retinal AstrocytesThesis5 The cell locations are kept at allcells.txt under the data directory. as a gray cell among multi-hue neighbors with 100% saturations. with a "new cell here" command of the mouse SaveFull Paper
2014Johnson, SM;Extracellular matrix improves mesenchymal stem cell growth and maintenance of stemness in serum free mediumThesisFreshly isolated human bone marrow mononuclear cells, containing BM-MSCs (primary cells) from 20-25 year old donors were purchased from ALLCELLS (Emeryville, CA). Greater SaveFull Paper
2014Tsai, HC;Molecular Mechanism in the Regulation of Human Th17 Cell Differentiation and FunctionThesisThe use of human cells in the study was periodically reviewed and approved by the Human Subject Research Review Committee (University of California, Davis). PBMCs were purchased from Allcells. LLC. Samples used in a single experiment were from differFull Paper
2014Spinler, KR;Membrane forces and key protein determinants of hematopoietic cell function: Lamins and myosin-II in hematopoiesis and CD47 in immunotherapy of cancerThesisFresh purified bone marrow-derived human CD34+ cells and total mononuclear cells were obtained from either the Penn Xenograft Core Facility or AllCells (Emeryville, CA). Cells from at least five different donors were used in this study. Purity of thFull Paper
2014Amir, ED;viSNE and Wanderlust, two algorithms for the visualization and analysis of high-dimensional single-cell dataThesisFresh, Ficoll-enriched human bone marrow was obtained from healthy donors from AllCells, Inc. (Emeryville, CA). Samples were obtained with informed consent in accordance with the Declaration of Helsinki and with accordance with Stanford University’sFull Paper
2014Qin, S;The use of biomaterials for cell function enhancement: acceleration of fibroblast migration and promotion of stem cell proliferationThesisHuman cord blood CD34+ cells were purchased from AllCells LLC and cultured in StemSpan SFEM media, 10% FBS, 100 ng/ml each of human SCF, TPO, and Flt-3L. Cells were infected with ICD-Notch or GFP control lentiviral particles then plated on hydrogelsFull Paper
2014Krzeszinski, JY;Wei, W;Huynh, H;Jin, Z;Wang, X;Chang, TC;Xie, XJ;He, L;Mangala, LS;Lopez-Berestein, G;Sood, AK;Mendell, JT;Wan, Y;miR-34a blocks osteoporosis and bone metastasis by inhibiting osteoclastogenesis and Tgif2Nature25043055Human PBMN cells (ReachBio) were differentiated into osteoclasts in α-MEM containing 10% FBS, 25 ng m1 −l MCSF, 50 ng m1 −l hRANKL, 1 μM Dexamethasome and 1 μM Save;odeoxyuridine (BrdU) cell proliferation assay kit (GE Healthcare) as described27. OFull Paper
2014Shah, N;Cortes, J;Martinelli, G;Smith, B;Clarke, E;Copland, M;Strauss, L;Talpaz, M;Dasatinib Plus Smoothened (SMO) Inhibitor BMS-833923 in Chronic Myeloid Leukemia (CML) with Resistance or Suboptimal Response to a Prior Tyrosine Kinase Inhibitor (TKI): Phase I Study CA180323BloodUniversity of California, San Francisco, San Francisco, CA++The University of Texas MD Anderson Cancer Center, Houston, TX++University of Bologna, Bologna, Italy++Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD++ReachBio LLCFull Paper
2014Chattopadhyay, R;Dyukova, E;Singh, NK;Ohba, M;Mobley, JA;Rao, GN;Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C ε-mediated zona occludens-1 phosphorylation at threonine 770/772The Journal of biological chemistry24338688The human peripheral blood CD14+ monocytes were purchased from ReachBio LLC (Seattle, WA), suspended in RPMI 1640 medium containing 50 μm 2-mercaptoethanol, 100 IU/ml penicillin, and 100 μg/ml streptomycin, and used for transmigration assay. To colleFull Paper
2014Winter, JM;Jacobson, P;Bullough, B;Christensen, AP;Boyer, M;Reems, JA;Long-term effects of cryopreservation on clinically prepared hematopoietic progenitor cell productsCytotherapy24910385CFC assays were performed 21, 22 with either commercially available MethoCult (StemCell Technologies Inc, Vancouver, Canada) or ColonyGel (ReachBio, LLC, Seattle, WA, USA) that had been internally validated to show comparable results. Briefly, HPC(A)Full Paper
2014Chandrasekaran, D;Nakamoto, B;Watts, KL;Kiem, HP;Papayannopoulou, T;Modeling promising nonmyeloablative conditioning regimens in nonhuman primatesHuman gene therapy24937231Transduced, mock-transduced, and BM white blood cell samples from transplanted macaques were cultured in semisolid methylcellulose medium for 12-14 days in the presence of rhIL-3, recombinant human erythropoietin (rhEPO), rhSCF, and recombinant humanFull Paper
2014Cook, GJ;Caudell, DL;Elford, HL;Pardee, TS;The efficacy of the ribonucleotide reductase inhibitor Didox in preclinical models of AMLPloS one25402485bated as indicated above for 24 hours with a titration of Didox. Cells were washed with PBS and resuspended in IMDM supplemented with 20% FBS, and placed in ColonyGel High Cytokine Formulation media (ReachBio). Experiments were performed in triplicatFull Paper
2014Byon, JC;Padilla, SM;Papayannopoulou, T;Deletion of Dicer in late erythroid cells results in impaired stress erythropoiesis in miceExperimental hematology24971698Colony Forming Assay Peripheral blood, spleen and bone marrow mononuclear cells were plated in methylcellulose containing hematopoietic growth factors (ReachBio, WA) as previously described [17]Full Paper
2014Li, X;Xu, X;Ji, T;Liu, Z;Gu, M;Hoang, BH;Zi, X;Dietary feeding of Flavokawain A, a Kava chalcone, exhibits a satisfactory safety profile and its association with enhancement of phase II enzymes in miceToxicology reports25379458ated, the yield and viability of cells were determined by Trypan blue exclusion method. A total number of 2 × 104 cells were seeded in a 6-well plate containing Colony Gel 1201 Mouse Base Medium (Reachbio), and then treated with FKA and Adriamycin aFull Paper
2014Erythroid, IVEOS;Progenitor Populations And Analogous Human Stress Erythroid ProgenitorsThesisHuman bone marrow mononuclear cells were purchased from Reachbio [26-‐200] and cultured in the stress erythropoiesis medium described above. On the indicated days, cells were SaveFull Paper
2014Smuda, CA;The Transcriptional Regulator Ikaros and Control of Mast Cell and Basophil DevelopmentThesisWe took advantage of the sorting strategies developed for quantitation of progenitor subtypes to isolate these populations for further analysis in solid culture media. IL-3 (5 ng/mL) and SCF (12.5 ng/mL) was added ColonyGel 1201 base methylcelluloseFull Paper
2013Singh, R;Low, ET;Ooi, LC;Ong-Abdullah, M;Ting, NC;Nagappan, J;Nookiah, R;Amiruddin, MD;Rosli, R;Manaf, MA;Chan, KL;Halim, MA;Azizi, N;Lakey, N;Smith, SW;Budiman, MA;Hogan, M;Bacher, B;Van Brunt, A;Wang, C;Ordway, JM;Sambanthamurthi, R;Martienssen, RA;The oil palm SHELL gene controls oil yield and encodes a homologue of SEEDSTICKNature23883930We thank Johannes Jansen (PRI, Wageningen, Netherlands) for assistance with JoinMap, and Beijing Genome Institute, The Genome Institute at Washington University, Tufts University Core Facility and the Arizona Genome Institute for mapping and genome sFull Paper
2013Ong, CT;Van Bortle, K;Ramos, E;Corces, VG;Poly(ADP-ribosyl)ation regulates insulator function and intrachromosomal interactions in DrosophilaCell24055367We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology for their help in performing Illumina sequencing of ChIP-Seq samples. This work was supported by U.S. Public Health Service Award R01 GM035463 from the NatFull Paper
2013Mukhopadhyay, S;Wen, X;Ratti, N;Loktev, A;Rangell, L;Scales, SJ;Jackson, PK;The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signalingCell23332756For the ISH experiments using radiolabeled probes, custom service from Phylogeny Inc. (Columbus, OH) was used. Both antisense and sense radiolabeled riboprobes were synthesized in vitro according to the manufacturer’s specifications (Ambion) and labeFull Paper
2013Locke, D;Bernstein, S;Lynch, F;Siami-Namini, K;Walling, J;Yonker, T;Yarranton, G;An IHC Screen For EphA3 Positive FFPE TumorsBloodNovember 15, 2013. An IHC Screen For EphA3 Positive FFPE Tumors. Darren Locke, PhD Darren Locke, PhD. 1. QualTek Molecular Laboratories, Newtown, USA,. 1. Search for other works by this author on: This Site. PubMed. GoogleFull Paper
2013Yang, J;Sung, E;Donlin-Asp, PG;Corces, VG;A subset of Drosophila Myc sites remain associated with mitotic chromosomes colocalized with insulator proteinsNat Commun23403565ChIP was performed with ~4 × 107 cells. Cells were cross-linked with 1% formaldehyde for 10 min at room temperature. Nuclear lysates were sonicated to generate 200– 1000 bp DNA fragments. ChIP was then performed with 6 μL of Drosophila α-Myc antibodyFull Paper
2013Day, K;Waite, LL;Thalacker-Mercer, A;West, A;Bamman, MM;Brooks, JD;Myers, RM;Absher, D;Differential DNA methylation with age displays both common and dynamic features across human tissues that are influenced by CpG landscapeGenome Biol.24034465;... samples All tissue samples were collected according to protocols and guidelines at their respective institutions. Iron deficient anemia buffy coat samples were commercially available and collected by Conversant Bio (Huntsville, AL, USA). Blood DFull Paper
2013Kellner, WA;Van Bortle, K;Li, L;Ramos, E;Takenaka, N;Corces, VG;Distinct isoforms of the Drosophila Brd4 homologue are present at enhancers, promoters and insulator sitesNucleic Acids Res.23945939Illumina adaptors (Illumina Cat# PE-102–1001) were titrated according to prepared DNA ChIP sample concentration and ligated with T4 ligase (NEB Cat# M0202S). Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (NFull Paper
2013Xing, F;Kobayashi, A;Okuda, H;Watabe, M;Pai, SK;Pandey, PR;Hirota, S;Wilber, A;Mo, YY;Moore, BE;Liu, W;Fukuda, K;Iiizumi, M;Sharma, S;Liu, Y;Wu, K;Peralta, E;Watabe, K;Reactive astrocytes promote the metastatic growth of breast cancer stem-like cells by activating Notch signalling in brainEMBO Mol Med23495140... ocyte (UC1) was a kind gift from Dr Russell Piper (University of California-San Francisco). Primary human breast tumour cells which maintained in xenograft tumour of NOD/SCID mouse were obtained from Conversant Biologics, Inc. shRNA-expressing leFull Paper
2013Park, S;Balasubramaniam, V;Sastry, S;Estimating pressure induced changes in vegetable tissue using in situ electrical conductivity measurement and instrumental analysisJournal of Food EngineeringPittsfield, MA, USA). The ends of the copper wires were soldered to the high pressure electrical feed through and electrically insulated using polyolefin heat shrink tubing (Q2-Z-1/8, Qualtek Electronics, Mentor, OH, USA). A typeFull Paper
2013Okuda, H;Xing, F;Pandey, PR;Sharma, S;Watabe, M;Pai, SK;Mo, YY;Iiizumi-Gairani, M;Hirota, S;Liu, Y;Wu, K;Pochampally, R;Watabe, K;miR-7 suppresses brain metastasis of breast cancer stem-like cells by modulating KLF4Cancer Res.23384942mixture. Breast tumor cells that were directly transplanted to animal only one generation without in vitro culture were obtained from Conversant Biologics. Stem cell populations were isolated as described previously (14). Iso ;... odium pyruvateFull Paper
2013Sah, S;Chen, L;Houghton, J;Kemppainen, J;Marko, AC;Zeigler, R;Latham, GJ;Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsiesGenome Med24001039Seventy-six FFPE blocks containing thyroid tumor biopsies were purchased from Asterand (Detroit, MI, USA). The remaining 39 FFPE tissue specimens were processed from colorectal cancer resections purchased from Folio Biosciences (Columbus, OH, USA) [1Full Paper
2013Soria, JC;Massard, C;Lazar, V;Ozoux, ML;Mery-Mignard, D;Deslandes, A;Tolcher, AW;A dose finding, safety and pharmacokinetic study of AVE1642, an anti-insulin-like growth factor-1 receptor (IGF-1R/CD221) monoclonal antibody, administered as a single agent and in combination with docetaxel in patients with advanced solid tumoursEur. J. Cancer23485230Tumour tissue: When possible, tumour tissue was collected before AVE1642 administration and at d21-d22 of cycle 1 to evaluate IGF-1R expression and signalling pathway markers, using validated immunohistochemistry assays, in Qualtek (USA)Full Paper
2013Rafaelsen, SH;Raeder, H;Fagerheim, AK;Knappskog, P;Carpenter, TO;Johansson, S;Bjerknes, R;Exome sequencing reveals FAM20c mutations associated with fibroblast growth factor 23-related hypophosphatemia, dental anomalies, and ectopic calcificationJ. Bone Miner. Res.23325605Genomic DNA was purified from blood using the QiaSymphony system (Qiagen, Hilden, Germany). Whole‐genome single‐nucleotide polymorphism (SNP) genotyping was performed with Genome‐Wide Human SNP 6.0 array (Affymetrix, Santa Clara, CA, USA). Whole‐exomFull Paper
2013Soler, VJ;Tran-Viet, KN;Galiacy, SD;Limviphuvadh, V;Klemm, TP;St Germain, E;Fournié, PR;Guillaud, C;Maurer-Stroh, S;Hawthorne, F;Suarez, C;Kantelip, B;Afshari, NA;Creveaux, I;Luo, X;Meng, W;Calvas, P;Cassagne, M;Arné, JL;Rozen, SG;Malecaze, F;Young, TL;Whole exome sequencing identifies a mutation for a novel form of corneal intraepithelial dyskeratosisJ. Med. Genet.23349227Seven micrograms of DNA were submitted to Hudson Alpha Laboratories (Huntsville, Alabama, USA) (http://www.hudsonalpha. org/) for deep sequencing processing to achieve an average coverage of 50× read depth. The libraries were prepared according to maFull Paper
2013Handorf, CR;Kulkarni, A;Grenert, JP;Weiss, LM;Rogers, WM;Kim, OS;Monzon, FA;Halks-Miller, M;Anderson, GG;Walker, MG;Pillai, R;Henner, WD;A multicenter study directly comparing the diagnostic accuracy of gene expression profiling and immunohistochemistry for primary site identification in metastatic tumorsAm. J. Surg. Pathol.23648464meeting the criteria were obtained as archived formalin-fixed, paraffin-embedded specimens from 4 institutions: the University of Tennessee (UT); Virginia Commonwealth University (VCU); Indiana University-Purdue University, Indianapolis (IUPUI); andFull Paper
2013Majumdar, S;Gong, EM;Di Vizio, D;Dreyfuss, J;Degraff, DJ;Hager, MH;Park, PJ;Bellmunt, J;Matusik, RJ;Rosenberg, JE;Adam, RM;Loss of Sh3gl2/endophilin A1 is a common event in urothelial carcinoma that promotes malignant behaviorNeoplasia23814487ILMN_1661491 [20]. Expression of Sh3gl2 protein was evaluated using commercially available bladder cancer tissue microarrays from either Folio Biosciences (Columbus, OH) or US Biomax, Inc (Rockville, MD). Specific immunohistochemicalFull Paper
2013Bowman, GL;Dodge, HH;Mattek, N;Barbey, AK;Silbert, LC;Shinto, L;Howieson, DB;Kaye, JA;Quinn, JF;Plasma omega-3 PUFA and white matter mediated executive decline in older adultsFront Aging Neurosci24379780Dr. Quinn has received honoraria for speaking from Pfizer, Novartis, and Forrest and for consulting from Phylogeny, Inc. Dr. Quinn is a co-inventor on a patent for the use of DHA for the treatment of Alzheimer's disease. Dr. Quinn receives compensatiFull Paper
2013Allaire, PD;Seyed Sadr, M;Chaineau, M;Seyed Sadr, E;Konefal, S;Fotouhi, M;Maret, D;Ritter, B;Del Maestro, RF;McPherson, PS;Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migrationJ. Cell. Sci.23264734Human brain tumor samples were obtained from the Montreal Neurological Institute Brain Tumor Tissue Bank. The experimental procedures were approved by the ethics committee of the Montreal Neurological Institute. The breast and squamous cell carcinomaFull Paper
2013Bohbot, JD;Durand, NF;Vinyard, BT;Dickens, JC;Functional Development of the Octenol Response in Aedes aegyptiFront Physiol23471139We wish to thank the following individuals: Dr. Shawn E. Levy and Nripesh Prasad at the Genomic Services Lab at HudsonAlpha Institute for Biotechnology for Illumina sequencing and data analyses; Dr. Gary Bauchan (The Electron and Confocal MicroscopeFull Paper
2013Premkumar, DR;Jane, EP;Agostino, NR;DiDomenico, JD;Pollack, IF;Bortezomib-induced sensitization of malignant human glioma cells to vorinostat-induced apoptosis depends on reactive oxygen species production, mitochondrial dysfunction, Noxa upregulation, Mcl-1 cleavage, and DNA damageMol. Carcinog.22086447time of tumor resection. We also obtained glioblastoma multiforme (GBM) primary cells from Conversant Biologics (Huntsville, AL), which were cultured in DMEM/F12 (Invitrogen, Carlsbad, CA). The established malignant gliomaFull Paper
2013Kinder, M;Greenplate, AR;Grugan, KD;Soring, KL;Heeringa, KA;McCarthy, SG;Bannish, G;Perpetua, M;Lynch, F;Jordan, RE;Strohl, WR;Brezski, RJ;Engineered protease-resistant antibodies with selectable cell-killing functionsJ. Biol. Chem.23986451Immunohistochemistry. All immunohistochemistry was performed by QualTek Molecular Laboratories (Newtown, PA). Four-micrometer sections were dewaxed through four changes of xylene (5 min each) followed by a graded alcohol series to distilled waterFull Paper
2013North, GB;Lynch, FH;Maharaj, FD;Phillips, CA;Woodside, WT;Leaf hydraulic conductance for a tank bromeliad: axial and radial pathways for moving and conserving waterFront Plant Sci23596446Full Paper
2013Park, S;Balasubramaniam, V;Sastry, S;Lee, J;Pressure–ohmic–thermal sterilization: A feasible approach for the inactivation of Bacillus amyloliquefaciens and Geobacillus stearothermophilus sporesInnovative Food Science & Emerging TechnologiesEach soldered part was electrically insulated using heat shrink tubing (Q2-F-1/16-01-QB6IN- 36, Qualtek, Mentor, OH, USA) and epoxy (2-t ® epoxy clear, Devcon, Danvers, MA, USA) to prevent short circuits and current loss to the pressure-transmittingFull Paper
2013Onsum, MD;Geretti, E;Paragas, V;Kudla, AJ;Moulis, SP;Luus, L;Wickham, TJ;McDonagh, CF;MacBeath, G;Hendriks, BS;Single-cell quantitative HER2 measurement identifies heterogeneity and distinct subgroups within traditionally defined HER2-positive patientsAm. J. Pathol.24035511Patient Samples. Sections (5 μm thick) of a breast disease spectrum TMA were obtained from Folio Biosciences (Powell, OH) (catalog number ARY-HH0137). Duplicate 1-mm tissue cores from 48 patients were represented on the TMAFull Paper
2013Liu, S;Wang, X;Sun, F;Zhang, J;Feng, J;Liu, H;Rajendran, KV;Sun, L;Zhang, Y;Jiang, Y;Peatman, E;Kaltenboeck, L;Kucuktas, H;Liu, Z;RNA-Seq reveals expression signatures of genes involved in oxygen transport, protein synthesis, folding, and degradation in response to heat stress in catfishPhysiol. Genomics23632418;Login to your account. Institutional Login. Sign In / Register. PHYSIOLOGY.ORG Skip main navigationClose Drawer MenuOpen Drawer Menu Home: Journals: AJP-Cell; AJP-Endo; AJP-GI; AJP-Heart; AJP-Lung; AJP-Regu; AJP-Renal; ; For each group, equalFull Paper
2013Kauffman, AL;Gyurdieva, AV;Mabus, JR;Ferguson, C;Yan, Z;Hornby, PJ;Alternative functional in vitro models of human intestinal epitheliaFront Pharmacol23847534n Majewski, Jason Ekert, and Kimberly Reese for help with iPSC maintenance culture. We also acknowledge the outstanding quality of immunohistochemistry performed by Steve Bernstein and Marko Sldanov, Qualtek Molecular Labs, Goleta, CA. References AlFull Paper
2013Lawley, B;Sims, IM;Tannock, GW;Whole-transcriptome shotgun sequencing (RNA-seq) screen reveals upregulation of cellobiose and motility operons of Lactobacillus ruminis L5 during growth on tetrasaccharides derived from barley β-glucanAppl. Environ. Microbiol.23851085Genomic comparisons were made in the expectation of understanding differential utilization of tetrasaccharides by L. ruminis strains. Paired-end reads were mapped to the publically available L. ruminis genomes: ATCC 25644 (did not utilize tetrasacchaFull Paper
2013Wang, R;Sun, L;Bao, L;Zhang, J;Jiang, Y;Yao, J;Song, L;Feng, J;Liu, S;Liu, Z;Bulk segregant RNA-seq reveals expression and positional candidate genes and allele-specific expression for disease resistance against enteric septicemia of catfishBMC Genomics24373586;... erformed using KAPA Library Quant Kits (Kapa Biosystems, USA) and dilution, the library were clustered 3 in one lane and sequenced on a Hiseq 2500 instrument with 100 bp paired end (PE) reads at the HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2013Xu, J;Li, Q;Xu, L;Wang, S;Jiang, Y;Zhao, Z;Zhang, Y;Li, J;Dong, C;Xu, P;Sun, X;Gene expression changes leading extreme alkaline tolerance in Amur ide (Leuciscus waleckii) inhabiting soda lakeBMC Genomics24094069;... ). Equal amounts of the high quality RNA samples from each tissue were then pooled for RNA-Seq. cDNA library construction and sequencing RNA-Seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2013Rokyta, DR;Wray, KP;Margres, MJ;The genesis of an exceptionally lethal venom in the timber rattlesnake (Crotalus horridus) revealed through comparative venom-gland transcriptomicsBMC Genomics23758969Sequencing and nonnormalized cDNA library preparation were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially as described by MortFull Paper
2013Sugihara, K;Shibata, TK;Takata, K;Kimura, T;Kanayama, N;Williams, R;Hatakeyama, S;Akama, TO;Kuo, CW;Khoo, KH;Fukuda, MN;Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycansFEBS Lett.23968720PolyLacs were collected and desalted using a Sephadex G-25 column and lyophilized. 2.7. Immunohistochemistry. Paraffin-embedded sections of human testis, epididymis, and ductus deferens tissues were obtained from Folio Biosciences (Columbus, OH)Full Paper
2013Gurudatta, BV;Yang, J;Van Bortle, K;Donlin-Asp, PG;Corces, VG;Dynamic changes in the genomic localization of DNA replication-related element binding factor during the cell cycleCell Cycle23624840The reaction was stopped by adding glycine to a final concentration of 0.125 mM. Chromatin was prepared by shearing the chromatin using sonication. Chromatin was immunoprecipitated using antibodies to DREF,35,40 and the DNA was used for library prepaFull Paper
2013Haugarvoll, K;Johansson, S;Tzoulis, C;Haukanes, BI;Bredrup, C;Neckelmann, G;Boman, H;Knappskog, PM;Bindoff, LA;MRI characterisation of adult onset alpha-methylacyl-coA racemase deficiency diagnosed by exome sequencingOrphanet J Rare Dis23286897Genealogical data from the patients, local parish registers and population censuses were used. Whole genome, single nucleotide polymorphism (SNP) genotyping was performed utilizing the Genome-Wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA) andFull Paper
2013O'Donnell, P;Ferguson, J;Shyu, J;Current, R;Rehage, T;Tsai, J;Christensen, M;Tran, HB;Chien, SS;Shieh, F;Wei, W;Lawrence, HJ;Wu, L;Schilling, R;Bloom, K;Maltzman, W;Anderson, S;Soviero, S;Analytic performance studies and clinical reproducibility of a real-time PCR assay for the detection of epidermal growth factor receptor gene mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancerBMC Cancer23621958(Wilmington, DE, USA), Asterand,Inc. (Detroit, MI, USA), BioServe (Beltsville, MD, USA), Conversant (Huntsville,AL, USA), Cureline Inc. (South San Francisco, CA, USA), Cytomyx (Lexington, MA,USA), Discovery Life Sciences, IncFull Paper
2013Peatman, E;Li, C;Peterson, BC;Straus, DL;Farmer, BD;Beck, BH;Basal polarization of the mucosal compartment in Flavobacterium columnare susceptible and resistant channel catfish (Ictalurus punctatus)Mol. Immunol.23895942; preserving the replicate pools for replicated QPCR validation. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25Full Paper
2013Sookruksawong, S;Sun, F;Liu, Z;Tassanakajon, A;RNA-Seq analysis reveals genes associated with resistance to Taura syndrome virus (TSV) in the Pacific white shrimp Litopenaeus vannameiDev. Comp. Immunol.23921257RNA-Seq library preparation and sequencing was outsourced and performed in the Genomic Services Lab of the HudsonAlpha Biotechnology Institute (Huntsville, AL, USA) as previously described (Li et al., 2012b; Sun et al., 2012). The cDNA libraries of TFull Paper
2013Macias, JD;Gerkin, RD;Locke, D;Macias, MP;Differential gene expression in cholesteatoma by DNA chip analysisLaryngoscope23670528From the Macias Otology (J.D.M.), Biomedical Research Program, the EAR Foundation of Arizona (J.D.M., M.P.M.), and Scientific Services, Banner Health Research Institute (R.D.G.), Phoenix, Arizona, USA; and QualTek Molecular Laboratories (D.L.), NewtoFull Paper
2013Infante, CR;Park, S;Mihala, AG;Kingsley, DM;Menke, DB;Pitx1 broadly associates with limb enhancers and is enriched on hindlimb cis-regulatory elementsDev. Biol.23201014Two independent biological replicates were used to generate two Illumina ChIP-Seq and two control libraries. All ChIP and input chromatin control libraries were produced using the Illumina ChIP-Seq DNA Sample Prep Kit (IP-102-1001) as directed by theFull Paper
2013Tzoulis, C;Johansson, S;Haukanes, BI;Boman, H;Knappskog, PM;Bindoff, LA;Novel SACS mutations identified by whole exome sequencing in a norwegian family with autosomal recessive spastic ataxia of Charlevoix-SaguenayPLoS ONE23785480Whole exome sequencing was performed at HudsonAlpha Institute for Biotechnology (Huntsville,AL) using Roche-NimbleGen Sequence Capture EZ Exome v2 kit and paired-end 100nt sequencing on the Illumina HiSeq [21].Full Paper
2013Xu, J;Ji, P;Wang, B;Zhao, L;Wang, J;Zhao, Z;Zhang, Y;Li, J;Xu, P;Sun, X;Transcriptome sequencing and analysis of wild Amur Ide (Leuciscus waleckii) inhabiting an extreme alkaline-saline lake reveals insights into stress adaptationPLoS ONE23573207;... lity RNA samples from each tissue were then pooled for cDNA synthesis and sequencing. cDNA Library Construction, Sequencing and Assembly RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2013Jiang, Y;Ninwichian, P;Liu, S;Zhang, J;Kucuktas, H;Sun, F;Kaltenboeck, L;Sun, L;Bao, L;Liu, Z;Generation of physical map contig-specific sequences useful for whole genome sequence scaffoldingPLoS ONE24205335One microliter ligation product was used as template for amplification with the following reaction cocktail: 1 µl 10X buffer, 1 U polymerase, 0.4 µl MgCl2 [50 mM], 0.8 µl dNTPs [2.5 mM], 2µl [5 µM] in-house-designed primers. Reactions were conductedFull Paper
2013Liu, L;Li, C;Su, B;Beck, BH;Peatman, E;Short-term feed deprivation alters immune status of surface mucosa in channel catfish (Ictalurus punctatus)PLoS ONE24023952;... ach condition, equal amounts of RNA from skin and gill were pooled to generate three pooled replicates for RNA-seq library construction. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2013Sun, F;Liu, S;Gao, X;Jiang, Y;Perera, D;Wang, X;Li, C;Sun, L;Zhang, J;Kaltenboeck, L;Dunham, R;Liu, Z;Male-biased genes in catfish as revealed by RNA-Seq analysis of the testis transcriptomePLoS ONE23874634;... Bioanalysis chip. Equal amount of total RNA from catfish testis of each age was pooled together into only one sample for use in RNA-Seq. RNA-Seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2013Garofalo, M;Jeon, YJ;Nuovo, GJ;Middleton, J;MiR-34a/c-dependent PDGFR-α/β downregulation inhibits tumorigenesis and enhances TRAIL-induced apoptosis in lung cancerPloS oneThe funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: GJN is an employee of Phylogeny, Inc. There are no patents, products in development or marketed productFull Paper
2013Lopez-Rios, F;Angulo, B;Gomez, B;Mair, D;Martinez, R;Conde, E;Shieh, F;Tsai, J;Vaks, J;Current, R;Lawrence, HJ;Gonzalez de Castro, D;Comparison of molecular testing methods for the detection of EGFR mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancerJ. Clin. Pathol.23386666Six FFPET specimens were purchased from INDIVUMED Inc. (Kensington, Maryland, USA), Cureline, Inc. (South San Francisco,California, USA), Bioserve (Beltsville, Maryland, USA), and ConversantBio (Huntsville, Alabama, USA). Study designFull Paper
2013Lamerz, J;Friedlein, A;Soder, N;Cutler, P;Döbeli, H;Determination of free desmosine in human plasma and its application in two experimental medicine studiesAnal. Biochem.23399390To validate the method, we used a plasma sample collection containing 30 patients with diagnosed COPD and 30 donors without a COPD diagnosis. This collection was obtained from Conversant Bio (Huntsville, AL, USA). ApartFull Paper
2013Pawar, H;Maharudraiah, J;Kashyap, MK;Sharma, J;Downregulation of cornulin in esophageal squamous cell carcinomaActa histochemicaThe ESCCs varied from tumor grade I to grade III from patients in the age group of 36-78 years. A second TMA set was obtained from FolioBio (Catalog no. ARY-HH0091, Powell, OH, USA) consisting of 40 ESCC cases with matched adjacent normal esophagealFull Paper
2013Adorno, M;Sikandar, S;Mitra, SS;Kuo, A;Nicolis Di Robilant, B;Haro-Acosta, V;Ouadah, Y;Quarta, M;Rodriguez, J;Qian, D;Reddy, VM;Cheshier, S;Garner, CC;Clarke, MF;Usp16 contributes to somatic stem-cell defects in Down's syndromeNature24025767Control bone marrow samples were obtained from AllCells, LLC. All samples were Control bone marrow samples were obtained from AllCells, LLC. Although we cannot unequivocally SaveFull Paper
2013Amir, el-AD;Davis, KL;Tadmor, MD;Simonds, EF;Levine, JH;Bendall, SC;Shenfeld, DK;Krishnaswamy, S;Nolan, GP;Pe'er, D;viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemiaNature biotechnology23685480Fresh, Ficoll-enriched human bone marrow was obtained from healthy donors from AllCells, Inc. (Emeryville, CA). Samples were obtained with informed consent in accordance with the SaveFull Paper
2013Meng, X;Su, RJ;Baylink, DJ;Neises, A;Kiroyan, JB;Lee, WY;Payne, KJ;Gridley, DS;Wang, J;Lau, KH;Li, G;Zhang, XB;Rapid and efficient reprogramming of human fetal and adult blood CD34+ cells into mesenchymal stem cells with a single factorCell research23478301University and written informed consent was obtained from all participants. CD34+ cells were purified with a CD34 Microbead Kit (Miltenyi Biotec, Auburn, CA, USA). Mobilized PB CD34+ cells were purchased from AllCells LLC (Emeryville, CA, USA)Full Paper
2013Yang, R;Liu, Y;Kelk, P;Qu, C;Akiyama, K;Chen, C;Atsuta, I;Chen, W;Zhou, Y;Shi, S;A subset of IL-17(+) mesenchymal stem cells possesses anti-Candida albicans effectCell research23266891Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells (Emeryville, CA, USA). Human bone marrow progenitor cells were enriched using fresh human bone marrow by RosetteSep® Human Bone Marrow ProgenitorFull Paper
2013Oshima, T;Sato, S;Kato, J;Ito, Y;Watanabe, T;Tsuji, I;Hori, A;Kurokawa, T;Kokubo, T;Nectin-2 is a potential target for antibody therapy of breast and ovarian cancersMolecular cancer23758976Human peripheral blood mononuclear cells (PBMC) from AllCells were cultured in RPMI1640 medium containing 10% FBS, 0.1 nM human IL-2 (DIACLONE Research), and 55 μM 2- SaveFull Paper
2013Takeishi, S;Matsumoto, A;Onoyama, I;Naka, K;Hirao, A;Nakayama, KI;Ablation of Fbxw7 eliminates leukemia-initiating cells by preventing quiescenceCancer cell23518349bone marrow mononuclear cells from treatment-naive patients in the chronic phase of CML and from healthy volunteers who had given informed consent were obtained from AllCells. SaveFull Paper
2013Deckert, J;Park, PU;Chicklas, S;Yi, Y;Li, M;Lai, KC;Mayo, MF;Carrigan, CN;Erickson, HK;Pinkas, J;Lutz, RJ;Chittenden, T;Lambert, JM;A novel anti-CD37 antibody-drug conjugate with multiple anti-tumor mechanisms for the treatment of B-cell malignanciesBlood24002446Frozen PBMCs from patients with CLL were obtained from AllCells (Emeryville, CA). For each sample, 5 × 10 5 cells were stained for indicated blood cell markers (Miltenyi) together with SaveFull Paper
2013Cutler, C;Multani, P;Robbins, D;Kim, HT;Le, T;Hoggatt, J;Pelus, LM;Desponts, C;Chen, YB;Rezner, B;Armand, P;Koreth, J;Glotzbecker, B;Ho, VT;Alyea, E;Isom, M;Kao, G;Armant, M;Silberstein, L;Hu, P;Soiffer, RJ;Scadden, DT;Ritz, J;Goessling, W;North, TE;Mendlein, J;Ballen, K;Zon, LI;Antin, JH;Shoemaker, DD;Prostaglandin-modulated umbilical cord blood hematopoietic stem cell transplantationBlood23996087Primary cells Cryopreserved human UCB CD34+ primary cells were purchased from AllCells or Stem Cell Technologies (Vancouver, BC, Canada)Full Paper
2013Malinge, S;Chlon, T;Doré, LC;Ketterling, RP;Tallman, MS;Paietta, E;Gamis, AS;Taub, JW;Chou, ST;Weiss, MJ;Crispino, JD;Figueroa, ME;Development of acute megakaryoblastic leukemia in Down syndrome is associated with sequential epigenetic changesBlood23980066Michigan, and the University of Michigan. Eight specimens of human CD34+ bone marrow cells were provided by the Stem Cell and Xenograft Core Facility of the University of Pennsylvania or purchased from AllCells (Emeryville, CA). These studies were peFull Paper
2013Kung Sutherland, MS;Walter, RB;Jeffrey, SC;Burke, PJ;Yu, C;Kostner, H;Stone, I;Ryan, MC;Sussman, D;Lyon, RP;Zeng, W;Harrington, KH;Klussman, K;Westendorf, L;Meyer, D;Bernstein, ID;Senter, PD;Benjamin, DR;Drachman, JG;McEarchern, JA;SGN-CD33A: a novel CD33-targeting antibody-drug conjugate using a pyrrolobenzodiazepine dimer is active in models of drug-resistant AMLBlood23770776For the primary AML xenograft model, NOD/SCID/IL-2Rgnull mice (NSG; The Jackson Laboratory, Bar Harbor, ME) were irradiated with 1 Gy 1 day before intravenous injection of 7 × 105 primary leukemia cells from a patient with relapsed AML (06227; AllCelFull Paper
2013Kojima, K;Kornblau, SM;Ruvolo, V;Dilip, A;Duvvuri, S;Davis, RE;Zhang, M;Wang, Z;Coombes, KR;Zhang, N;Qiu, YH;Burks, JK;Kantarjian, H;Shacham, S;Kauffman, M;Andreeff, M;Prognostic impact and targeting of CRM1 in acute myeloid leukemiaBlood23564911Normal marrow CD34 + cells from healthy donors aged 18 to 55 were obtained from AllCells (Emeryville, CA). The demographics and clinical characteristics of the patient population SaveFull Paper
2013Lee, J;Shieh, JH;Zhang, J;Liu, L;Zhang, Y;Eom, JY;Morrone, G;Moore, MA;Zhou, P;Improved ex vivo expansion of adult hematopoietic stem cells by overcoming CUL4-mediated degradation of HOXB4Blood23520338Each irradiated mouse (5 mice/group) was transplanted via tail vein injection with 3 × 105 CD34+ G-CSF-mobilized adult HSCs (AllCells, LLC, Emeryville, CA) treated with 2 rounds of purified recombinant wild-type or degradation-resistant HOXB4 proteinFull Paper
2013Bhagat, TD;Zhou, L;Sokol, L;Kessel, R;Caceres, G;Gundabolu, K;Tamari, R;Gordon, S;Mantzaris, I;Jodlowski, T;Yu, Y;Jing, X;Polineni, R;Bhatia, K;Pellagatti, A;Boultwood, J;Kambhampati, S;Steidl, U;Stein, C;Ju, W;Liu, G;Kenny, P;List, A;Bitzer, M;Verma, A;miR-21 mediates hematopoietic suppression in MDS by activating TGF-β signalingBlood23390194Bone marrow CD34+ cells from various normal donors were also obtained from AllCells (Emeryville, CA). HS-5 cell line (CRL-11882) was purchased from ATCC (Manassas, VA). TGF-β1 was bought from R&D Systems (Minneapolis, MN). The phos-SMAD2 (S465/467) aFull Paper
2013Kumkhaek, C;Aerbajinai, W;Liu, W;Zhu, J;Uchida, N;Kurlander, R;Hsieh, MM;Tisdale, JF;Rodgers, GP;MASL1 induces erythroid differentiation in human erythropoietin-dependent CD34+ cells through the Raf/MEK/ERK pathwayBlood23327923CD34+ cells were obtained from commercial sources (Lonza, Walkerville, MD, or AllCells, Emeryville, CA). Cells were thawed and washed into StemSpan serum-free expansion medium (SFEM) (StemCell Technologies, Vancouver, BC, Canada) and then seeded in SFull Paper
2013Wong, KY;Yim, RL;Kwong, YL;Leung, CY;Hui, PK;Cheung, F;Liang, R;Jin, DY;Chim, CS;Epigenetic inactivation of the MIR129-2 in hematological malignanciesJournal of hematology & oncology23406679MIR124-1 and MIR203 were previously described [14,16]. Mononuclear cell DNA from 15 healthy donors [8 bone marrow, 3 peripheral blood, 3 CD19-sorted peripheral blood, and 1 CD138-sorted bone marrow (AllCells, CA, USA)] were used as negative control,Full Paper
2013Richardson, CL;Delehanty, LL;Bullock, GC;Rival, CM;Tung, KS;Kimpel, DL;Gardenghi, S;Rivella, S;Goldfarb, AN;Isocitrate ameliorates anemia by suppressing the erythroid iron restriction responseThe Journal of clinical investigation23863711The unique sensitivity of early red cell progenitors to iron deprivation, known as the erythroid iron restriction response, serves as a basis for human anemias globally. This response SaveFull Paper
2013McBrian, MA;Behbahan, IS;Ferrari, R;Su, T;Huang, TW;Li, K;Hong, CS;Christofk, HR;Vogelauer, M;Seligson, DB;Kurdistani, SK;Histone acetylation regulates intracellular pHMolecular cell23201122H1 cells were maintained in mTeSR1 medium (STEMCELL Technologies #05850) on Matrigel. T cells were purchased from AllCells (PB009-1F) and were maintained in RPMI-1640 (ATCC #30-2001) with 10% FBS (Hyclone #SV3001403) that was heat inactivated by treaFull Paper
2013Kuroda, Y;Wakao, S;Kitada, M;Murakami, T;Nojima, M;Dezawa, M;Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cellsNature protocols23787896For example, when we purchased bone marrow from ALLCELLS, which is imported from the United States to Japan and typically takes 5-7 d to arrive, the number of mononuclear cells SaveFull Paper
2013Cheng, DT;Ma, C;Niewoehner, J;Dahl, M;Tsai, A;Zhang, J;Gonsiorek, W;Apparsundaram, S;Pashine, A;Ravindran, P;Jung, J;Hang, J;Allard, J;Bitter, H;Tribouley, C;Narula, S;Wilson, S;Fuentes, ME;Thymic stromal lymphopoietin receptor blockade reduces allergic inflammation in a cynomolgus monkey model of asthmaThe Journal of allergy and clinical immunology23810153Single donor human cord blood-derived CD34 + cells were purchased from AllCells (Emeryville, Calif). Cells were cultured for 8 weeks in serum-free complete medium (StemPro-34 SaveFull Paper
2013Leone, E;Morelli, E;Di Martino, MT;Amodio, N;Foresta, U;Gullà, A;Rossi, M;Neri, A;Giordano, A;Munshi, NC;Anderson, KC;Tagliaferri, P;Tassone, P;Targeting miR-21 inhibits in vitro and in vivo multiple myeloma cell growthClinical cancer research : an official journal of the American Association for Cancer Research23446999Imgenex) by standard procedures (37, 38). hBMSCs were obtained by long-term culture of bone marrow mononuclear cells (39). For coculture, 1 × 105 ppMM cells were seeded on 2 × 104 hBMSCs for 24 to 48 hours in 96-well plates. RNA samples of normal heaFull Paper
2013Kuhne, MR;Mulvey, T;Belanger, B;Chen, S;Pan, C;Chong, C;Cao, F;Niekro, W;Kempe, T;Henning, KA;Cohen, LJ;Korman, AJ;Cardarelli, PM;BMS-936564/MDX-1338: a fully human anti-CXCR4 antibody induces apoptosis in vitro and shows antitumor activity in vivo in hematologic malignanciesClinical cancer research : an official journal of the American Association for Cancer Research23213054Binding Buffer 10× concentrate, 7-aminoactinomycin D (7-AAD), and Annexin V-APC from BD Biosciences; phycoerythrin (PE)-conjugated goat anti-human antibody from Jackson ImmunoResearch (Cat. 109-116-098). AML peripheral blood mononuclear cells (PBMC)Full Paper
2013Corbin, AS;O'Hare, T;Gu, Z;Kraft, IL;Eiring, AM;Khorashad, JS;Pomicter, AD;Zhang, TY;Eide, CA;Manley, PW;Cortes, JE;Druker, BJ;Deininger, MW;KIT signaling governs differential sensitivity of mature and primitive CML progenitors to tyrosine kinase inhibitorsCancer research23887971Normal bone marrow mononuclear cells (MNC) were from AllCells. Cell selection was as described previously (details in Supplementary Methods; ref. 17). SaveFull Paper
2013Ara, T;Nakata, R;Sheard, MA;Shimada, H;Buettner, R;Groshen, SG;Ji, L;Yu, H;Jove, R;Seeger, RC;DeClerck, YA;Critical role of STAT3 in IL-6-mediated drug resistance in human neuroblastomaCancer research23633489AmpFISTR Identifier PCR kit and GeneMapper ID v. 3.2 (Applied Biosystems). Human BMMSC were purchased from AllCells LLC. Monocytes of normal healthy donors were obtained from peripheral blood and separated by Ficoll density gradient centrifugation usFull Paper
2013Wei, Y;Chen, R;Dimicoli, S;Bueso-Ramos, C;Neuberg, D;Pierce, S;Wang, H;Yang, H;Jia, Y;Zheng, H;Fang, Z;Nguyen, M;Ganan-Gomez, I;Ebert, B;Levine, R;Kantarjian, H;Garcia-Manero, G;Global H3K4me3 genome mapping reveals alterations of innate immunity signaling and overexpression of JMJD3 in human myelodysplastic syndrome CD34+ cellsLeukemia23538751BMs from healthy individuals were obtained from AllCells (Emeryville, CA, USA). Isolation of CD34+ cells was performed using MicroBead Kit (Miltenyi, Bergisch Gladbach, Germany) SaveFull Paper
2013Rio-Machin, A;Ferreira, BI;Henry, T;Gómez-López, G;Agirre, X;Alvarez, S;Rodriguez-Perales, S;Prosper, F;Calasanz, MJ;Martínez, J;Fonseca, R;Cigudosa, JC;Downregulation of specific miRNAs in hyperdiploid multiple myeloma mimics the oncogenic effect of IgH translocations occurring in the non-hyperdiploid subtypeLeukemia23174883CD138-positive snap-frozen plasma cell pellets (ALLCELLS, Emeryville, CA, USA) were DNA from healthy CD138+ snap-frozen plasma cell pellets (ALLCELLS, Emeryville, CA, USA) SaveFull Paper
2013Díaz-Beyá, M;Navarro, A;Ferrer, G;Díaz, T;Gel, B;Camós, M;Pratcorona, M;Torrebadell, M;Rozman, M;Colomer, D;Monzo, M;Esteve, J;Acute myeloid leukemia with translocation (8;16)(p11;p13) and MYST3-CREBBP rearrangement harbors a distinctive microRNA signature targeting RET proto-oncogeneLeukemia23022987In addition, CD34+ cells from the bone marrow of three healthy donor subjects were purchased from AllCells (Emeryville, CA, USA). All patients provided their written informed consent SaveFull Paper
2013Hayashi, Y;Hirai, H;Kamio, N;Yao, H;Yoshioka, S;Miura, Y;Ashihara, E;Fujiyama, Y;Tenen, DG;Maekawa, T;C/EBPβ promotes BCR-ABL-mediated myeloid expansion and leukemic stem cell exhaustionLeukemia22948537Frozen samples of human bone marrow cells from healthy donors or untreated CP-CML patients were purchased from AllCells LLC (Emeryville, CA, USA). In all cases, written informed Save;[方紘] 慢性期 CML 患者および健常人ドナーの骨髄細胞検体は AllCells 社より購入した. マウス 血幹細胞の細胞 でFull Paper
2013Lin, X;Rice, KL;Buzzai, M;Hexner, E;Costa, FF;Kilpivaara, O;Mullally, A;Soares, MB;Ebert, BL;Levine, R;Licht, JD;miR-433 is aberrantly expressed in myeloproliferative neoplasms and suppresses hematopoietic cell growth and differentiationLeukemia22864358Human CD34+ cells were obtained from adult bone marrow (AllCells, Emeryville, CA, USA) and cultured in StemSpan serum-free expansion media (SFEM; Stem Cell Technologies, SaveFull Paper
2013Shin, JW;Spinler, KR;Swift, J;Chasis, JA;Mohandas, N;Discher, DE;Lamins regulate cell trafficking and lineage maturation of adult human hematopoietic cellsProceedings of the National Academy of Sciences of the United States of America24191023Commercially available primary human cells from marrow were obtained from either AllCells, Inc. (Emeryville, CA) or anonymous donors to the University of Pennsylvania Stem Cell SaveFull Paper
2013Xie, J;Zhang, H;Yea, K;Lerner, RA;Autocrine signaling based selection of combinatorial antibodies that transdifferentiate human stem cellsProceedings of the National Academy of Sciences of the United States of America23613575Differentiation of CD34+ Cells in Culture. Fresh bone marrow CD34+ cells were purchased from Allcells (catalog no. ABM010). The purity is greater than 96% as determined by FACS. The cells were washed with SFEM (Stem Cell 09600) and kept in SFEM withFull Paper
2013Hong, KU;Li, QH;Guo, Y;Patton, NS;Moktar, A;Bhatnagar, A;Bolli, R;A highly sensitive and accurate method to quantify absolute numbers of c-kit+ cardiac stem cells following transplantation in miceBasic research in cardiology23549981Female human peripheral mononuclear cells (hPBMCs) were purchased from AllCells, LLC (Emeryville, CA). Aliquots of 105 hPBMCs (in 50 μl PBS) were stored at −20 °C until use. For isolation of genomic DNA, QIAamp DNA Mini Kit (Qiagen) was used accordinFull Paper
2013Ramakrishnan, P;Clark, PM;Mason, DE;Peters, EC;Hsieh-Wilson, LC;Baltimore, D;Activation of the transcriptional function of the NF-κB protein c-Rel by O-GlcNAc glycosylationScience signaling23982206Biotechnology. The anti-FLAG antibody was from Sigma. The anti-O-GlcNAc antibodies RL2 and CTD 110.6 were from Abcam and Covance, respectively. Cells Jurkat, Jurkat T-REx, Ramos, EL4, MEFs, and HEK 293T cells were grown as previously described (23).Full Paper
2013Isgro, J;Gupta, S;Jacek, E;Pavri, T;Duculan, R;Kim, M;Kirou, KA;Salmon, JE;Pernis, AB;Enhanced rho-associated protein kinase activation in patients with systemic lupus erythematosusArthritis and rheumatism23508371Cryopreserved vials of human cord blood CD4+ T lymphocytes were obtained from Lonza Poietics, Immune Cell Systems, and AllCells, LLC. CD4+ T cell purity was 95%. Cells were thawed according to manufacturer’s instructions and cultured in LGM-3 (Lonza)Full Paper
2013Allakhverdi, Z;Comeau, MR;Armant, M;Agrawal, R;Woodfolk, JA;Sehmi, R;Howie, KJ;Gauvreau, GM;Delespesse, G;Mast Cell-Activated Bone Marrow Mesenchymal Stromal Cells Regulate Proliferation and Lineage Commitment of CD34(+) Progenitor CellsFrontiers in immunology24381572ibody were used at 10 μg/ml, as in our previous study (4). Primary human bone marrow-derived mesenchymal stromal cells Human BM-derived mesenchymal stromal cells were established from BM samples (AllCells, Emeryville, CA, USA) by culture in minimumFull Paper
2013Wu, Q;Wang, H;Zhao, X;Shi, Y;Jin, M;Wan, B;Xu, H;Cheng, Y;Ge, H;Zhang, Y;Identification of G-protein-coupled receptor 120 as a tumor-promoting receptor that induces angiogenesis and migration in human colorectal carcinomaOncogene23851494ECMatrix (50 μl, Merck KGaA ) was distributed per well in a 96-well plate. HUVECs (ALLCELLS, Shanghai, China, 2 × 104 cells/well) suspended in 100 μl of medium supplemented with 0.5% FBS were added to each well and cultured in triplicate with 150 μlFull Paper
2013Li, C;Takahashi, C;Zhang, L;Huseni, M;Stankovich, B;Mashhedi, H;Lee, J;French, D;Anderson, JE;Kim, D;Howell, K;Brauer, MJ;Kowanetz, M;Yan, Y;Humke, E;Ebens, A;Hampton, G;Lackner, MR;Hegde, P;Jia, S;Development of a robust flow cytometry-based pharmacodynamic assay to detect phospho-protein signals for phosphatidylinositol 3-kinase inhibitors in multiple myelomaJournal of translational medicine23522020le myeloma Li Congfen 1 li.congfen@gene.com Takahashi Chikara 1 takahashi.chikara@gene.com Zhang Liangxuan 1 zhang.liangxuan@gene.com Huseni Mahrukh 1 mahrukhh@gene.com Stankovich Basha 2 bstankovich@allcells.com Mashhedi Haider 1 mashhedh@gene.com LFull Paper
2013Nazarov, C;Lo Surdo, J;Bauer, SR;Wei, CH;Assessment of immunosuppressive activity of human mesenchymal stem cells using murine antigen specific CD4 and CD8 T cells in vitroStem cell research & therapy24406271by the Association for Assessment and Accreditation of Laboratory Animal Care International. All experiments were performed according to institutional guidelines. Cell culture hMSCs were purchased (AllCells, Emeryville, CA, USA) at passage one. AccoFull Paper
2013Ledford, KJ;Zeigler, F;Bartel, RL;Ixmyelocel-T, an expanded multicellular therapy, contains a unique population of M2-like macrophagesStem cell research & therapy24405629For the generation of M1 and M2 macrophages, human peripheral blood monocytes from healthy donors were purchased (AllCells LLC., Alameda, USA). The cells were polarized into SaveFull Paper
2013Vanella, L;Sodhi, K;Kim, DH;Puri, N;Maheshwari, M;Hinds, TD;Bellner, L;Goldstein, D;Peterson, SJ;Shapiro, JI;Abraham, NG;Increased heme-oxygenase 1 expression in mesenchymal stem cell-derived adipocytes decreases differentiation and lipid accumulation via upregulation of the canonical Wnt signaling cascadeStem cell research & therapy23497794Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA, USA). After thawing, mononuclear cells were resuspended in an α-minimal essential SaveFull Paper
2013McNamara, LA;Onafuwa-Nuga, A;Sebastian, NT;Riddell, J;Bixby, D;Collins, KL;CD133+ hematopoietic progenitor cells harbor HIV genomes in a subset of optimally treated people with long-term viral suppressionThe Journal of infectious diseases23554378and 7-aminoactinomycin D (7-AAD). Healthy donor BMMCs (AllCells) were stained with R-phycoerythrin-conjugated anti-CD133, R-phycoerythrin-Cy7-conjugated anti-CD34 (BD), and 7-AAD. Samples were analyzed on a BD FACSCantoFull Paper
2013Moy, LY;Jia, Y;Caniga, M;Lieber, G;Gil, M;Fernandez, X;Sirkowski, E;Miller, R;Alexander, JP;Lee, HH;Shin, JD;Ellis, JM;Chen, H;Wilhelm, A;Yu, H;Vincent, S;Chapman, RW;Kelly, N;Hickey, E;Abraham, WM;Northrup, A;Miller, T;Houshyar, H;Crackower, MA;Inhibition of spleen tyrosine kinase attenuates allergen-mediated airway constrictionAmerican journal of respiratory cell and molecular biology23889698+ cells (5 x 10 6 /vial, AllCells) were thawed and added into 50 ml polypropylene conical tube with 100 µL of 1mg/ml DNase I (StemCell). 15.5 ml of pre-warmed Dulbecco's modified SaveFull Paper
2013Ye, L;Muench, MO;Fusaki, N;Beyer, AI;Wang, J;Qi, Z;Yu, J;Kan, YW;Blood cell-derived induced pluripotent stem cells free of reprogramming factors generated by Sendai viral vectorsStem cells translational medicine23847002Frozen human peripheral blood mobilized CD34+ cells were purchased from AllCells (Emeryville, CA, http://www.allcells.com). After thawing, CD34+ cells were cultured with serum-free medium (SFM) as described by Ye and coworkers [24, 29], containing 50Full Paper
2013Hall, SR;Jiang, Y;Leary, E;Yavanian, G;Eminli, S;O'Neill, DW;Marasco, WA;Identification and isolation of small CD44-negative mesenchymal stem/progenitor cells from human bone marrow using elutriation and polychromatic flow cytometryStem cells translational medicine23847000Fresh, mobilized leukapheresis products were purchased from AllCells (Emeryville, CA, http://www.allcells.com) or collected from healthy volunteers at NeoStem Laboratory (Cambridge, MA, http://www.neostem.com) under an institutional review board-apprFull Paper
2013Mendelson, A;Cheung, Yk;Paluch, K;Chen, M;Kong, K;Tan, J;Dong, Z;Sia, SK;Mao, JJ;Competitive stem cell recruitment by multiple cytotactic cuesLab on a chip23364311Human bone marrow MSCs were isolated from fresh adult donor samples (AllCells, Emeryville, CA) using our prior methods. Briefly, the bone marrow sample was incubated with the SaveFull Paper
2013Cairo, C;Sagnia, B;Cappelli, G;Colizzi, V;Leke, RG;Leke, RJ;Pauza, CD;Human cord blood γδ T cells expressing public Vγ2 chains dominate the response to bisphosphonate plus interleukin-15Immunology23181340fraction of CBMC was reserved for cell culture and 0·5 × 107 to 1 × 107 were lysed for RNA extraction; remaining cells were frozen at 1 × 107 CBMC/ml, in 90% fetal bovine serum (FBS), 10% DMSO freezing medium. Some CBMC specimens were purchased fromFull Paper
2013Kim, DH;Puri, N;Sodhi, K;Falck, JR;Abraham, NG;Shapiro, J;Schwartzman, ML;Cyclooxygenase-2 dependent metabolism of 20-HETE increases adiposity and adipocyte enlargement in mesenchymal stem cell-derived adipocytesJournal of lipid research23293373Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α-MEM, Save;ates: hypertension, the metabolic syndrome, and diabetes. MATERIALSFull Paper
2013Luo, Y;Kleiboeker, S;Deng, X;Qiu, J;Human parvovirus B19 infection causes cell cycle arrest of human erythroid progenitors at late S phase that favors viral DNA replicationJournal of virology24049177Cells and virus. (i) CD36+ EPCs. Human bone marrow CD34+ hematopoietic stem/progenitor cells (HSCs) were positively isolated using a direct immunomagnetic CD34+ MicroBead labeling system and were purchased from AllCells, LLC (Alameda, CA; catalog noFull Paper
2013Shen, Y;Zeng, L;Zhu, A;Blanc, T;Patel, D;Pennello, A;Bari, A;Ng, S;Persaud, K;Kang, YK;Balderes, P;Surguladze, D;Hindi, S;Zhou, Q;Ludwig, DL;Snavely, M;Removal of a C-terminal serine residue proximal to the inter-chain disulfide bond of a human IgG1 lambda light chain mediates enhanced antibody stability and antibody dependent cell-mediated cytotoxicitymAbs23567210drogenase (LDH) activity from the lysed targeted cells (A431 transfected with receptor target X, A431x) was monitored in the presence freshly isolated normal peripheral blood mononuclear cells (PBMC, AllCells, PB002) upon the treatment with λ or λdSFull Paper
2013Zhao, Y;Wang, J;Wang, M;Sun, P;Chen, J;Jin, X;Zhang, H;Activation of bone marrow-derived mesenchymal stromal cells-a new mechanism of defocused low-energy shock wave in regenerative medicineCytotherapy24199590Human umbilical vein endothelial cells (HUVEC) were purchased from Allcells (Shanghai, China) and maintained in EGM2 medium (CC-3162, Lonza, Basel, Switzerland). HUVEC SaveFull Paper
2013Jones, M;Varella-Garcia, M;Skokan, M;Bryce, S;Schowinsky, J;Peters, R;Vang, B;Brecheisen, M;Startz, T;Frank, N;Nankervis, B;Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum SystemCytotherapy23992670Initially, six human bone marrow aspirates supplied by normal adult donors, who gave informed consent through a certified clinical collection facility (AllCells, Alameda, CA, USA), were SaveFull Paper
2013Glen, KE;Workman, VL;Ahmed, F;Ratcliffe, E;Stacey, AJ;Thomas, RJ;Production of erythrocytes from directly isolated or Delta1 Notch ligand expanded CD34+ hematopoietic progenitor cells: process characterization, monitoring and implications for manufactureCytotherapy23831361Background aims Economic ex vivo manufacture of erythrocytes at 10 12 cell doses requires an efficiently controlled bio-process capable of extensive proliferation and high terminal SaveFull Paper
2013Song, L;Kauss, MA;Kopin, E;Chandra, M;Ul-Hasan, T;Miller, E;Jayandharan, GR;Rivers, AE;Aslanidi, GV;Ling, C;Li, B;Ma, W;Li, X;Andino, LM;Zhong, L;Tarantal, AF;Yoder, MC;Wong, KK;Tan, M;Chatterjee, S;Srivastava, A;Optimizing the transduction efficiency of capsid-modified AAV6 serotype vectors in primary human hematopoietic stem cells in vitro and in a xenograft mouse model in vivoCytotherapy23830234CD34+ and umbilical cord blood CD34+ cells were either purchased from AllCells or obtained under a City of Hope Institutional Review Board approved protocol isolated as described by Chatterjee et al. [7], and maintained in either StemSpan SFEM mediumFull Paper
2013Blackwood, E;Epler, J;Yen, I;Flagella, M;O'Brien, T;Evangelista, M;Schmidt, S;Xiao, Y;Choi, J;Kowanetz, K;Ramiscal, J;Wong, K;Jakubiak, D;Yee, S;Cain, G;Gazzard, L;Williams, K;Halladay, J;Jackson, PK;Malek, S;Combination drug scheduling defines a "window of opportunity" for chemopotentiation of gemcitabine by an orally bioavailable, selective ChK1 inhibitor, GNE-900Molecular cancer therapeutics23873850For the bone marrow assay, fresh human bone marrow-derived cells were obtained from AllCells and plated at a density of 1 million cells/mL in Human Methylcellulose Complete SaveFull Paper
2013Jekle, A;Yoon, J;Zuck, M;Najafi, R;Wang, L;Shiau, T;Francavilla, C;Rani, SA;Eitzinger, C;Nagl, M;Anderson, M;Debabov, D;NVC-422 inactivates Staphylococcus aureus toxinsAntimicrobial agents and chemotherapy23208720Normal human peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA) and maintained in RPMI 1640 medium supplemented with 10% fetal bovine SaveFull Paper
2013Winkler, DG;Faia, KL;DiNitto, JP;Ali, JA;White, KF;Brophy, EE;Pink, MM;Proctor, JL;Lussier, J;Martin, CM;Hoyt, JG;Tillotson, B;Murphy, EL;Lim, AR;Thomas, BD;Macdougall, JR;Ren, P;Liu, Y;Li, LS;Jessen, KA;Fritz, CC;Dunbar, JL;Porter, JR;Rommel, C;Palombella, VJ;Changelian, PS;Kutok, JL;PI3K-δ and PI3K-γ inhibition by IPI-145 abrogates immune responses and suppresses activity in autoimmune and inflammatory disease modelsChemistry & biology24211136Primary Human B and T Cell Proliferation Assays Human peripheral blood CD19+ B cells and CD3+ T cells were purchased from Allcells and proliferation assays were conducted as described in Supplemental Experimental ProceduresFull Paper
2013Liao, C;Hsu, J;Kim, Y;Hu, DQ;Xu, D;Zhang, J;Pashine, A;Menke, J;Whittard, T;Romero, N;Truitt, T;Slade, M;Lukacs, C;Hermann, J;Zhou, M;Lucas, M;Narula, S;DeMartino, J;Tan, SL;Selective inhibition of spleen tyrosine kinase (SYK) with a novel orally bioavailable small molecule inhibitor, RO9021, impinges on various innate and adaptive immune responses: implications for SYK inhibitors in autoimmune disease therapyArthritis research & therapy24286216y kits (BD Biosciences). IgE-induced histamine release in human mast cells The method has been reported previously by our group [9]. Briefly, human cord blood-derived CD34+ hematopoietic stem cells (AllCells, Emeryville, CA, USA) were cultured in aFull Paper
2013Tsai, HC;Wu, R;Cholera toxin directly enhances IL-17A production from human CD4+ T cellsJournal of immunology (Baltimore, Md. : 1950)24043897PBMCs were purchased from AllCells. According to the manufacturers' protocols, total CD4 + T cells were positively selected from PBMCs using magnetic CD4 MicroBeads (Miltenyi SaveFull Paper
2013Sohn, SJ;Barrett, K;Van Abbema, A;Chang, C;Kohli, PB;Kanda, H;Smith, J;Lai, Y;Zhou, A;Zhang, B;Yang, W;Williams, K;Macleod, C;Hurley, CA;Kulagowski, JJ;Lewin-Koh, N;Dengler, HS;Johnson, AR;Ghilardi, N;Zak, M;Liang, J;Blair, WS;Magnuson, S;Wu, LC;A restricted role for TYK2 catalytic activity in human cytokine responses revealed by novel TYK2-selective inhibitorsJournal of immunology (Baltimore, Md. : 1950)23894201IL-23 assay. IL-23 was obtained from R&D Systems and was used at a concentration that generated ∼50-90% of the maximal IL-23-induced signal in the assay (i.e., EC50-90 of IL-23). CD4 CD45RO+ T cells obtained from AllCells (Emeryville, CA) were culturFull Paper
2013Yokley, BH;Selby, ST;Posch, PE;A stimulation-dependent alternate core promoter links lymphotoxin α expression with TGF-β1 and fibroblast growth factor-7 signaling in primary human T cellsJournal of immunology (Baltimore, Md. : 1950)23547113Human T cells were purchased (Allcells, Emeryville, CA, USA) or were purified from PBMCs isolated from fresh whole blood with lymphocyte separation medium (Ficoll® and hypaque; Mediatech Inc, Manassas, VA, USA). Briefly, whole blood was brought to roFull Paper
2013Fitzgerald, DC;Fonseca-Kelly, Z;Cullimore, ML;Safabakhsh, P;Saris, CJ;Zhang, GX;Rostami, A;Independent and interdependent immunoregulatory effects of IL-27, IFN-β, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitisJournal of immunology (Baltimore, Md. : 1950)23455508PBMCs were isolated using Ficoll-Paque Plus density centrifugation; cord blood cells were obtained from AllCells. CD4 + and CD14 + cells were purified by immunomagnetic SaveFull Paper
2013Heuck, CJ;Mehta, J;Bhagat, T;Gundabolu, K;Yu, Y;Khan, S;Chrysofakis, G;Schinke, C;Tariman, J;Vickrey, E;Pulliam, N;Nischal, S;Zhou, L;Bhattacharyya, S;Meagher, R;Hu, C;Maqbool, S;Suzuki, M;Parekh, S;Reu, F;Steidl, U;Greally, J;Verma, A;Singhal, SB;Myeloma is characterized by stage-specific alterations in DNA methylation that occur early during myelomagenesisJournal of immunology (Baltimore, Md. : 1950)23408834Eight samples from normal donors without known malignancies were obtained commercially from AllCells (Emeryville, CA). All normal donor samples had a purity of ≥85%. SaveFull Paper
2013Jiang, Z;Hu, X;Yu, H;Xu, Y;Wang, L;Chen, H;Chen, H;Wu, R;Zhang, Z;Xiang, C;Webster, KA;Wang, JA;Human endometrial stem cells confer enhanced myocardial salvage and regeneration by paracrine mechanismsJournal of cellular and molecular medicine23837896the supplemental information. Cell sources Human BMMSCs from three donors were purchased from Cyagen Bioscience (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Human EnSCs from threeFull Paper
2013He, X;Xu, Z;Wang, B;Zheng, Y;Gong, W;Huang, G;Zhang, L;Li, Y;He, F;Upregulation of thrombomodulin expression by activation of farnesoid X receptor in vascular endothelial cellsEuropean journal of pharmacology24041925The primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells, LLC (Shanghai, China) and were cultured in the medium supplied by AllCells with 10% SaveFull Paper
2013Larusch, GA;Merkulova, A;Mahdi, F;Shariat-Madar, Z;Sitrin, RG;Cines, DB;Schmaier, AH;Domain 2 of uPAR regulates single-chain urokinase-mediated angiogenesis through β1-integrin and VEGFR2American journal of physiology. Heart and circulatory physiology23709605HUVECs were cultured in 96-well microtiter plates on gelatin at 1 × 10 4 cells/well for 24 h in EGM from AllCells. Cells were then washed with serum-free medium without growth factor SaveFull Paper
2013Chen, Y;Chitapanarux, T;Wu, J;Soon, RK;Melton, AC;Yee, HF;Inducible NOS mediates CNP-induced relaxation of intestinal myofibroblastsAmerican journal of physiology. Gastrointestinal and liver physiology23348803NPR Western blots were performed by AllCells (Emeryville, CA) with the following antibodies: anti-mouse NPR-A (clone ab116680; Abcam, Cambridge, MA), anti-mouse NPR-B (clone SaveFull Paper
2013Koestler, DC;Christensen, B;Karagas, MR;Marsit, CJ;Langevin, SM;Kelsey, KT;Wiencke, JK;Houseman, EA;Blood-based profiles of DNA methylation predict the underlying distribution of cell types: a validation analysisEpigenetics23903776As previously described,10,18 our reference set (S0) consisted of sorted, normal, human, peripheral blood leukocyte subtypes purchased from AllCells. Leukocytes were isolated from different, anonymous, non-diseased individuals’ whole-blood by magnetiFull Paper
2013Harb, W;Fan, A;Tran, T;Danila, DC;Keys, D;Schwartz, M;Ionescu-Zanetti, C;Mutational Analysis of Circulating Tumor Cells Using a Novel Microfluidic Collection Device and qPCR AssayTranslational oncology24151533Healthy donor peripheral blood was obtained from a commercial supplier (AllCells, Emeryville, CA) under Institutional Review Board (IRB) consent. Blood was drawn into EDTA tubes ( SaveFull Paper
2013Shahaduzzaman, M;Golden, JE;Green, S;Gronda, AE;Adrien, E;Ahmed, A;Sanberg, PR;Bickford, PC;Gemma, C;Willing, AE;A single administration of human umbilical cord blood T cells produces long-lasting effects in the aging hippocampusAge (Dordrecht, Netherlands)23263793Cryopreserved HUCB MNC fraction and its component subfractions (CD3+, CD4+, CD8+, CD14+, and CD133+) were obtained from AllCells, LLC. HUCB cells were thawed in a 37 C SaveFull Paper
2013de Lichtervelde, L;Boitano, AE;Wang, Y;Krastel, P;Petersen, F;Cooke, MP;Schultz, PG;Eupalinilide E inhibits erythropoiesis and promotes the expansion of hematopoietic progenitor cellsACS chemical biology23441826Human mPB CD34 + cells were purified from fresh human leukophoresed mobilized peripheral blood (AllCells) using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following SaveFull Paper
2013Xiao, L;Hung, KC;Takahashi, TT;Joo, KI;Lim, M;Roberts, RW;Wang, P;Antibody-mimetic ligand selected by mRNA display targets DC-SIGN for dendritic cell-directed antigen deliveryACS chemical biology23427768Human PBMC-derived DCs were generated and purified as previously described, (46, 47) except that human PBMCs were from HLA-A2+ patients (AllCells, LLC.). Briefly, plastic-adherent cell fractions of human PBMCs were grown in RPMI 1640 with 10% heat-inFull Paper
2013Čokić, VP;Smith, RD;Biancotto, A;Noguchi, CT;Puri, RK;Schechter, AN;Globin gene expression in correlation with G protein-related genes during erythroid differentiationBMC genomics23425329isolation system (Miltenyi Biotec, Auburn, CA). Commercial FL- (Cambrex Bio Science, Inc., Walkersville, MD), CB-, BM- and granulocyte-colony stimulating factor (G-CSF) stimulated mobilized PB- (mPB, AllCells LLC, Berkeley, CA) derived CD34+ cells arFull Paper
2013Musiychuk, K;Sivalenka, R;Jaje, J;Bi, H;Flores, R;Shaw, B;Jones, RM;Golovina, T;Schnipper, J;Khandker, L;Sun, R;Li, C;Kang, L;Voskinarian-Berse, V;Zhang, X;Streatfield, S;Hambor, J;Abbot, S;Yusibov, V;Plant-produced human recombinant erythropoietic growth factors support erythroid differentiation in vitroStem cells and development23517237Umbilical cord blood-derived CD34 + cells were obtained from AllCells and maintained in the StemSpan Serum-Free Expansion Medium (StemCell Technologies) containing 1 μM SaveFull Paper
2013Ramalingam, S;London, V;Kandavelou, K;Cebotaru, L;Guggino, W;Civin, C;Chandrasegaran, S;Generation and genetic engineering of human induced pluripotent stem cells using designed zinc finger nucleasesStem cells and development22931452Human primary CBMNCs were purchased from Allcells, Inc. The frozen CBMNCs were thawed and then cultured in the serum free medium (SFM) supplemented with cytokines and a SaveFull Paper
2013Chen, J;Shi, ZD;Ji, X;Morales, J;Zhang, J;Kaur, N;Wang, S;Enhanced osteogenesis of human mesenchymal stem cells by periodic heat shock in self-assembling peptide hydrogelTissue engineering. Part A23072422Human bone marrow (BM) from the iliac crest of a 27-year-old donor was purchased (AllCells LLC, Berkeley, CA). Mononuclear cells were enriched and retrieved with RosetteSep MSC enrichment cocktail (StemCell Technologies, Vancouver, Canada) accordingFull Paper
2013Wei, N;Yu, Y;Joshi, V;Schmidt, T;Qian, F;Salem, AK;Stanford, C;Hong, L;Glucocorticoid receptor antagonist and siRNA prevent senescence of human bone marrow mesenchymal stromal cells in vitroCell and tissue research23963647Fresh human bone marrows, donated by total six healthy male and female donors from 22 to 43 years of age, were purchased from Allcells (Emeryville, CA). Mononuclear cells were isolated using density gradient centrifugation (Histopaque-1.077; Sigma, SFull Paper
2013Hutchinson, AT;Malik, A;Berkahn, MB;Agostino, M;To, J;Tacchi, JL;Djordjevic, SP;Turnbull, L;Whitchurch, CB;Edmundson, AB;Jones, DR;Raison, RL;Ramsland, PA;Formation of assemblies on cell membranes by secreted proteins: molecular studies of free λ light chain aggregates found on the surface of myeloma cellsThe Biochemical journal23822104We have described the presence of cell-membrane-associated κFLCs (free immunoglobulin light chains) on the surface of myeloma cells. Notably, the anti-κFLC mAb (monoclonal SaveFull Paper
2013Choudhary, R;Baturin, D;Fosmire, S;Freed, B;Porter, CC;Knockdown of HPRT for selection of genetically modified human hematopoietic progenitor cellsPloS one23555045Human umbilical cord blood samples unsuitable for clinical use were obtained from the ClinImmune Labs or were purchased from AllCells (Emeryville, CA). CD34 + cells were isolated SaveFull Paper
2013Song, L;Li, X;Jayandharan, GR;Wang, Y;Aslanidi, GV;Ling, C;Zhong, L;Gao, G;Yoder, MC;Ling, C;Tan, M;Srivastava, A;High-efficiency transduction of primary human hematopoietic stem cells and erythroid lineage-restricted expression by optimized AAV6 serotype vectors in vitro and in a murine xenograft model in vivoPloS one23516552fetal bovine serum (FBS; Sigma, St. Louis, MO), 100 µg/ml of penicillin and 100 U/ml of streptomycin (Invitrogen, Grand Island, NY). Human cord blood CD34+ cells and CD36+ cells were purchased from AllCells (AllCells Technologies, Emeryville, CA) andFull Paper
2013Wang, T;Choi, E;Monaco, MC;Campanac, E;Medynets, M;Do, T;Rao, P;Johnson, KR;Elkahloun, AG;Von Geldern, G;Johnson, T;Subramaniam, S;Hoffman, DA;Hoffman, D;Major, E;Nath, A;Derivation of neural stem cells from human adult peripheral CD34+ cells for an autologous model of neuroinflammationPloS one24303066marker to detect mesenchymal cells, as CD29 and CD271, were used to stain the isolated CD34+ to further asses their purity. CD34+ cell culture and transfection Cord blood CD34+ cells (purchased from Allcells, Emeryville, California) or adult CD34+ cFull Paper
2013Xiao, F;Kim, YC;Wen, H;Luo, J;Chen, P;Cowan, K;Wang, SM;The genome of polymorphonuclear neutrophils maintains normal coding sequencesPloS one24250807loid differentiation. Our study concludes that genomic DNA from myeloid lineage cells can be safely used in genetic studies. Methods Ethics Statement: The cells used for the study were obtained from AllCells LLC (http://www.allcells.com/, EmeryvilleFull Paper
2013Kornblau, SM;Qutub, A;Yao, H;York, H;Qiu, YH;Graber, D;Ravandi, F;Cortes, J;Andreeff, M;Zhang, N;Coombes, KR;Proteomic profiling identifies distinct protein patterns in acute myelogenous leukemia CD34+CD38- stem-like cellsPloS one24223100tcome. This population lack samples from the 10-15% of AML cases that are CD34 negative. As normal controls, 1x106 CD34+ cells were collected from 10 different unstimulated normal bone marrow donors (AllCells, Emeryville, CA http://www.allcells.com/)Full Paper
2013Chai, ZT;Kong, J;Zhu, XD;Zhang, YY;Lu, L;Zhou, JM;Wang, LR;Zhang, KZ;Zhang, QB;Ao, JY;Wang, M;Wu, WZ;Wang, L;Tang, ZY;Sun, HC;MicroRNA-26a inhibits angiogenesis by down-regulating VEGFA through the PIK3C2α/Akt/HIF-1α pathway in hepatocellular carcinomaPloS one24194905ecco’s modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA) containing 10% fetal bovine serum (FBS) in a humidified incubator at 37°C with an atmosphere of 5% CO2. HUVECs were purchased from Allcells (Shanghai, China) and cultured in EndothelialFull Paper
2013Tarantal, AF;Lee, CC;Kukis, DL;Cherry, SR;Radiolabeling human peripheral blood stem cells for positron emission tomography (PET) imaging in young rhesus monkeysPloS one24098579CD34+ hPBSC were obtained from a commercial source (AllCells, Berkeley, CA) and cryopreserved using a controlled rate cryopreservation protocol in aliquots as previously reported [ SaveFull Paper
2013DeBoever, C;Reid, EG;Smith, EN;Wang, X;Dumaop, W;Harismendy, O;Carson, D;Richman, D;Masliah, E;Frazer, KA;Whole transcriptome sequencing enables discovery and analysis of viruses in archived primary central nervous system lymphomasPloS one24023918To serve as a positive control a cord blood sample was purchased from AllCells (Emeryville, CA). Peripheral blood mononuclear cells (PBMC) were extracted from peripheral blood SaveFull Paper
2013Dimicoli, S;Wei, Y;Bueso-Ramos, C;Yang, H;Dinardo, C;Jia, Y;Zheng, H;Fang, Z;Nguyen, M;Pierce, S;Chen, R;Wang, H;Wu, C;Garcia-Manero, G;Overexpression of the toll-like receptor (TLR) signaling adaptor MYD88, but lack of genetic mutation, in myelodysplastic syndromesPloS one23976989Bone marrows from healthy individuals were obtained from AllCells (Emeryville, CA). CD34+ cells were isolated from fresh bone marrow specimens of patients with MDS using SaveFull Paper
2013Dolley-Sonneville, PJ;Romeo, LE;Melkoumian, ZK;Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditionsPloS one23940553Prior to the initiation of the multi-passage study we evaluated several plating densities based on recommendation of the manufacturer of hBM-MSCs used in the study (AllCells) and SaveFull Paper
2013Holmberg, RC;Gindlesperger, A;Stokes, T;Lopez, D;Hyman, L;Freed, M;Belgrader, P;Harvey, J;Li, Z;Akonni TruTip(®) and Qiagen(®) methods for extraction of fetal circulating DNA--evaluation by real-time and digital PCRPloS one23936545’s Circulating Nucleic Acid Kit. In order to determine the comparative extraction efficiency of the two methods on a large set of male fetal specimens, 19 maternal plasma samples were collected by AllCells (Emeryville, CA) or StemExpress (PlacervillFull Paper
2013Hsiao, SC;Liu, H;Holstlaw, TA;Liu, C;Francis, CY;Francis, MB;Real time assays for quantifying cytotoxicity with single cell resolutionPloS one23826123To each well were added 180 µL of freshly drawn human blood (AllCells, Inc.). After 16 h of incubation at 37C, the samples were visualized directly. Because the target cells are all SaveFull Paper
2013Zhang, C;Wu, X;Zhang, M;Zhu, L;Zhao, R;Xu, D;Lin, Z;Liang, C;Chen, T;Chen, L;Ren, Y;Zhang, J;Qin, N;Zhang, X;Small molecule R1498 as a well-tolerated and orally active kinase inhibitor for hepatocellular carcinoma and gastric cancer treatment via targeting angiogenesis and mitosis pathwaysPloS one23755206nd by the vendors and subjected to in vitro assays between passages 8∼15, the cell lines for animal studies were between passages 5∼10. Human umbilical vein endothelial cell (HUVEC) obtained from Allcells (Emeryville, CA) was kept in EGM-2 (LONZA, AlFull Paper
2013Mendel, TA;Clabough, EB;Kao, DS;Demidova-Rice, TN;Durham, JT;Zotter, BC;Seaman, SA;Cronk, SM;Rakoczy, EP;Katz, AJ;Herman, IM;Peirce, SM;Yates, PA;Pericytes derived from adipose-derived stem cells protect against retinal vasculopathyPloS one23741506tient-derived lipoaspirate and further purified with serial passaging and sub-culturing, as we and others have previously shown [22]. Human bone marrow derived stem cells (hBMSCs) were purchased from Allcells (#MSC-001F). Bovine retinae and retinal mFull Paper
2013Su, RJ;Baylink, DJ;Neises, A;Kiroyan, JB;Meng, X;Payne, KJ;Tschudy-Seney, B;Duan, Y;Appleby, N;Kearns-Jonker, M;Gridley, DS;Wang, J;Lau, KH;Zhang, XB;Efficient generation of integration-free ips cells from human adult peripheral blood using BCL-XL together with Yamanaka factorsPloS one23704989ied by MACS as described previously. [40] MNCs were obtained by standard density gradient centrifugation with Ficoll-Hypaque (1.077 g/ml) at room temperature. Some PBMNCs were directly purchased from AllCells (Emeryville, CA). The age of male and femFull Paper
2013Evseenko, D;Latour, B;Richardson, W;Corselli, M;Sahaghian, A;Cardinal, S;Zhu, Y;Chan, R;Dunn, B;Crooks, GM;Lysophosphatidic acid mediates myeloid differentiation within the human bone marrow microenvironmentPloS one23696850emical analyses. The murine stromal cell lines MS5 and OP9 (American Type Culture Collection (ATCC, Rockville, MD, USA) or primary human bone marrow derived mesenchymal stromal cells (passage 2-3) (AllCells Inc, Emeryville CA) were seeded into chambeFull Paper
2013Melton, AC;Melrose, J;Alajoki, L;Privat, S;Cho, H;Brown, N;Plavec, AM;Nguyen, D;Johnston, ED;Yang, J;Polokoff, MA;Plavec, I;Berg, EL;O'Mahony, A;Regulation of IL-17A production is distinct from IL-17F in a primary human cell co-culture model of T cell-mediated B cell activationPloS one23505568Frozen vials of positively selected primary normal human CD19+ B cells and negatively selected CD4+ T cells were obtained from AllCells (Emeryville, CA). PBMC were isolated from SaveFull Paper
2013Angevine, K;Wuescher, L;Mensah-Osman, E;Loss of menin mediated by endothelial cells treated with CoPP is associated with increased maturation of adipocytesAdipocyte24052896, 10 mM l-glutamine, 0.75 units/mL heparin sulfate, 1 μg/mL hydrocortisone, 50 μg/mL ascorbic acid, and 2% fetal bovine serum. Bone marrow derived mesenchymal stem cells (MSCs) were purchased from AllCells and maintained at 37 °C and 5% CO2 in α-memFull Paper
2013Omana-Zapata, I;Oreizy, F;Mosqueda, F;Salemenik, M;Lin, A;Wen, JL;Varro, R;Lee-Haynes, L;Segurado, OG;Performance of a novel BD stem cell enumeration kit on two flow cytometry systemsInternational journal of laboratory hematology23190563Blood collection program and one frozen leukapheresis aliquot were enrolled for Linearity. Twenty-seven fresh leukapheresis aliquots from healthy mobilized adult donors were enrolled in the stability evaluation. All leukapheresis aliquots, fresh andFull Paper
2013Seki, K;Hisada, T;Kawata, T;Kamide, Y;Dobashi, K;Yamada, M;Mori, M;Okajima, F;Ishizuka, T;Oxidative stress potentially enhances FcεRI-mediated leukotriene C4 release dependent on the late-phase increase of intracellular glutathione in mast cellsBiochemical and biophysical research communications23998930Human cord blood CD34 + cells were purchased from ALLCELLS (Emeryville, CA) and cultured in complete medium consisting of RPMI1640 (Life Technologies, Carlsbad, CA) SaveFull Paper
2013Li, S;Garrett-Bakelman, FE;Akalin, A;Zumbo, P;Levine, R;To, BL;Lewis, ID;Brown, AL;D'Andrea, RJ;Melnick, A;Mason, CE;An optimized algorithm for detecting and annotating regional differential methylationBMC bioinformatics23735126Ten acute myeloid leukemia (AML) de-identified patient samples enriched for myeloblast cells and five normal bone marrow (NBM) samples (purchased from AllCells) were used in the SaveFull Paper
2013Ohkawa, S;Kamei, N;Kamei, G;Shi, M;Adachi, N;Deie, M;Ochi, M;Magnetic targeting of human peripheral blood CD133+ cells for skeletal muscle regenerationTissue engineering. Part C, Methods23298291)-mobilized human peripheral blood-derived-MNCs by using a direct immunomagnetic CD133 MicroBeads labeling and a magnetic cell sorting system, were purchased from AllCells( SaveFull Paper
2013Kim, J;Zanjani, ED;Jeanblanc, CM;Goodrich, AD;Hematti, P;Generation of CD34+ cells from human embryonic stem cells using a clinically applicable methodology and engraftment in the fetal sheep modelExperimental hematology23612043For generation of BM CD14 + cell-derived macrophages, CD14 + cells were isolated from BM aspirates of normal donors (AllCells) and harvested after 1 week (n = 6). For generation of SaveFull Paper
2013Cherukuri, A;Patton, K;Gasser, RA;Zuo, F;Woo, J;Esser, MT;Tang, RS;Adults 65 years old and older have reduced numbers of functional memory T cells to respiratory syncytial virus fusion proteinClinical and vaccine immunology : CVI23239796A pooled human reference plasma standard (AllCells, LLC, Emeryville, CA) was included on each plate for comparison, and all plates were incubated for 1 h. A SulfoTag-labeled goat SaveFull Paper
2013Kim, DH;Liu, J;Bhat, S;Benedict, G;Lecka-Czernik, B;Peterson, SJ;Ebraheim, NA;Heck, BE;Peroxisome proliferator-activated receptor delta agonist attenuates nicotine suppression effect on human mesenchymal stem cell-derived osteogenesis and involves increased expression of heme oxygenase-1Journal of bone and mineral metabolism22945906Frozen bone marrow mononuclear cells were purchased from AllCells (Emeryville, CA, USA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential SaveFull Paper
2013Li, L;Wang, Y;Chen, J;Cheng, B;Hu, J;Zhou, Y;Gao, X;Gao, L;Mei, X;Sun, M;Zhang, Z;Song, H;An Engineered Arginase FC Protein Inhibits Tumor Growth In Vitro and In VivoEvidence-based complementary and alternative medicine : eCAM23737831umbilical cord vein were purchased from Allcells Inc. A confluent monolayer of synchronized HUVECS was scraped with a razor blade as described in [14]. After wounding, the cells SaveFull Paper
2013Tummala, S;Titus, M;Wilson, L;Wang, C;Ciatto, C;Thill, G;Foster, D;Li, C;Szabo, Z;Guttman, A;Bettencourt, B;Jayaraman, M;Deroot, J;Kocisko, D;Pollard, S;Charisse, K;Kuchimanchi, S;Hinkle, G;Milstein, S;Meyers, R;Wu, SL;Karger, BL;Rossomando, A;Evaluation of exogenous siRNA addition as a metabolic engineering tool for modifying biopharmaceuticalsBiotechnology progress2317273512K media containing 10% fetal bovine serum. Fresh human normal PBMCs were purchased from AllCells (Emeryville, CA). Approximately 1 ×105 Jeko-1 cells were pre-incubated with RNAi-treated or control antibodies at varying concentrations ranging from 0Full Paper
2013Rocha, LR;Veiga, DF;e Oliveira, PR;Song, EH;Ferreira, LM;Health Service Quality Scale: Brazilian Portuguese translation, reliability and validityBMC health services research23327598Business School, Avinguda de Pearson, 21, 08034, Barcelona, Spain Find articles by Paulo Rocha e Oliveira [https://pubmed.ncbi.nlm.nih.gov/?term=e%20Oliveira%20PR%5BAuthor%5D] ELAINE HORIBE SONG 4AllCells LLC, 5858 Horton Street, Emeryville, CA, 9Full Paper
2013Ross, KM;McDonald-Jones, G;Miller, GE;Oxytocin does not attenuate the ex vivo production of inflammatory cytokines by lipopolysaccharide-activated monocytes and macrophages from healthy male and female donorsNeuroimmunomodulation23899661For MP experiments, we purchased MO-derived, in vitro matured MPs from a cell supplier (AllCells, Emeryville, CA). All MPs used here were matured from a single donor's peripheral Save;Background: Oxytocin (OT) is a neuropeptide shown to attenuate iFull Paper
2013Wu, Y;Yang, Y;Yang, P;Gu, Y;Zhao, Z;Tan, L;Zhao, L;Tang, T;Li, Y;The osteogenic differentiation of PDLSCs is mediated through MEK/ERK and p38 MAPK signalling under hypoxiaArchives of oral biology23806288The bone marrow stromal stem cells (BMSSCs) used as controls were obtained from a commercially available resource (AllCells LLC, Berkeley, CA, USA). SaveFull Paper
2013Miller, P;Stabilization of memory States by stochastic facilitating synapsesJournal of mathematical neuroscience24314108S is a scaled version of s, accounting for the totalfeedback conductance, S = W s , where W is the sum of connection strengths of allcells and held fixed when N is varied. SaveFull Paper
2013Zhan, H;Cardozo, C;Yu, W;Wang, A;Moliterno, AR;Dang, CV;Spivak, JL;MicroRNA deregulation in polycythemia vera and essential thrombocythemia patientsBlood cells, molecules & diseases23265742the peripheral blood CD34+ cells were 95% positive both for CD34 and CD38, were dimly positive for CD117, were CD41 negative, and were primarily in G0/G1 of the cell cycle [31]. Peripheral blood CD34+ cells from healthy controls were purchased from AFull Paper
2013Li, H;Xie, W;Gore, ER;Montoute, MN;Bee, WT;Zappacosta, F;Zeng, X;Wu, Z;Kallal, L;Ames, RS;Pope, AJ;Benowitz, A;Erickson-Miller, CL;Development of phenotypic screening assays for γ-globin induction using primary human bone marrow day 7 erythroid progenitor cellsJournal of biomolecular screening24163393All cryopreserved human bone marrow CD34 + cells used here were obtained from AllCells (Emeryville, CA) and were generally from different donors. The CD34 + cells were cultured SaveFull Paper
2013Schwimmer, LJ;Huang, B;Giang, H;Cotter, RL;Chemla-Vogel, DS;Dy, FV;Tam, EM;Zhang, F;Toy, P;Bohmann, DJ;Watson, SR;Beaber, JW;Reddy, N;Kuan, HF;Bedinger, DH;Rondon, IJ;Discovery of diverse and functional antibodies from large human repertoire antibody librariesJournal of immunological methods23454004The source RNA and cDNA used to amplify the V-genes was purchased from AllCells and Cureline. The E. coli strain TG1 (Lucigen) was used for all molecular cloning, phage SaveFull Paper
2013Rosa, M;Pragasam, P;Saremian, J;Aoalin, A;Graf, W;Mohammadi, A;The unsatisfactory ThinPrep® Pap Test™: analysis of technical aspects, most common causes, and recommendations for improvementDiagnostic cytopathology22833404Fourth slide displays "halo effect" in which allcells are located at the periphery of the slide. This represents a technical preparation problem that can be resolved in the majority of cases SaveFull Paper
2013Vainer, ED;Meir, K;Furman, M;Semenenko, I;Konikoff, F;Vainer, GW;Characterization of novel CD55 isoforms expression in normal and neoplastic tissuesTissue antigens23692281RNA was obtained from Ambion (Austin, TX), Asterand (Detroit, MI), AllCells (Emeryville, CA), Genomics Collaborative (Seracare; Cambridge, MA), Biochain (Newark, CA) and Ichilov medical center (Tel-aviv, Israel). CRC cell lines: colo205, DLD-1, HCT11Full Paper
2013Muluk, NB;Arikan, OK;Atasoy, P;Kiliç, R;Yalçinozan, ET;Inducible nitric oxide synthase (iNOS) in sinonasal polyp pathogenesisB-ENT24273952the allcells group of inflammatory cells, there were PMNCs, MNCs, and fibroblasts. Higher iNOS expression in the allcells Thus, iNOS expression in the allcells group was mainly due to SaveFull Paper
2013Wagner, LA;Wang, S;Wayner, EA;Christensen, C;Perkins, SJ;Ward, GW;Weiss, RB;Dunn, DM;Redd, MJ;Spangrude, GJ;Gleich, GJ;Developing and mature human granulocytes express ELP 6 in the cytoplasmHuman antibodies24284306R&D Systems) or 2 U/ml epoietin-α [18]. Collection of de-identified UCMC was approved by the University of Utah’s Institutional Review Board. Bone marrow mononuclear cells (BMMC) were purchased from AllCells (Emeryville, CA)Full Paper
2013Köllmer, M;Buhrman, JS;Zhang, Y;Gemeinhart, RA;Markers Are Shared Between Adipogenic and Osteogenic Differentiated Mesenchymal Stem CellsJournal of developmental biology and tissue engineering24013643Human bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque TM PLUS solution followed by cell SaveFull Paper
2013Hu, K;Slukvin, I;Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectorsMethods in molecular biology (Clifton, N.J.)23546755Human cord blood mononuclear cells (AllCells, CA, USA). 12. Human bone marrow mononuclear cells (AllCells, CA, USA). 13. Human bone marrow cells from patient with CML (AllCells, CA, USA). 14. Human cord blood CD34+ cells (AllCells, CA, USA). 15.Full Paper
2013Chu, EM;Tai, DC;Beer, JL;Hill, JS;Macrophage heterogeneity and cholesterol homeostasis: classically-activated macrophages are associated with reduced cholesterol accumulation following treatment with oxidized LDLBiochimica et biophysica acta23142249CD14 + human peripheral blood monocytes (AllCells) were cultured in RPMI 1640 complete media (Hyclone) containing 10% FBS (Hyclone), 2% sodium bicarbonate, 1% sodium SaveFull Paper
2013Arora, M;Cell Culture Media: A ReviewMaterials and Methodsary cells. CELLS MEDIA Endothelial cells Human Endothelial-SFM (Life Technologies), Endothelial Basal Media, EndoGRO-LS Complete Media Kit (MilliporeSigma), HUVEC Basal Medium CB HUVEC (AllCells), Endothelial Cell Medium (ScienCell)Full Paper
2013Mirshokraie, S;Mesbah, A;Pattabiraman, K;Efficient JavaScript Mutation Testing2013 IEEE Sixth International Conference on Software Testing, Verification and Validationfunction calls setup to set the dimension of all DOM elements with a class name of allCells; Assuming that the number of DOM elements with class name allCells is 9 (line 3 in Figure 1), SaveFull Paper
2013Fox, S;Adachi, T;Stillwell, W;A QUINONE-ASSISTED PHOTOFORMATION OF ENERGY-RICH CHEMICAL BONDSSolar Energy International ProgressIn severalconferences , biochemists , chemicalengineers , andothershavediscusse dhow to industrializephotosyn thesis and how to industrializethe ATP cyclethatoperates in allcells . T SaveFull Paper
2013Margules, D;Flynn, J;Cooper, C;CALCITONIN-LIKE PEPTIDE IN PRO-OPIOCORTIN: FUNCTIONAL ASPECTSAdvances in Endogenous and Exogenous Opioidscells of the anterior pituitary and in allcells of the intermediate pituitary . EXCESS IN OBESE cells of the anterior pituitary and in allcells of the intermediate pituitary . EXCESS IN OBESE SaveFull Paper
2013Brodie, G;CONSTRUCTED WETLANDS FOR TREATING ACID DRAINAGE AT TENNESSEE VALLEY AUTHORITY COAL FACILITIESConstructed Wetlands in Water Pollution ControlS tagedtreatmentusingse veralcells may providea more efficientsystemsincec hemical and hydraulicoverloading of allcells would be minimized. Acid drainage treatment Water depth SaveFull Paper
2013Masuda, H;Asahara, T;Isolation of Colony-Forming Endothelial Progenitor CellsManual of Research Techniques in Cardiovascular Medicineapproval of the institutional ethical committees of the Cord Blood and/or Clinical Investigation Committee. Alternatively, isolated MNCs, CD34+ , or CD133+ cells in UCB, PB, or BM can be purchased (e.g. ALLCELLSTM: http://www.allcells.com)Full Paper
2013Drew, H;Analysis of Macrophage-ThesisLPS, bovine CS and bovine aggrecan were purchased from SigmaAldrich (St. Louis, MO). Human monocytes (AllCells, LCC, Emeryville, CA) were cultured in RPMI-1640 with 10% fetal bovine serum. Bovine keratan sulfate proteoglycan (KSPG) was purchased fFull Paper
2013Adams, GN;Prolylcarboxypeptidase protects from vascular dysfunction and promotes vascular repairThesisHUVEC were purchased from AllCells (Emeryville, CA) and grown on gelatin coated plates in CSC media (Cells Systems; Kirkland, WA). Cells were grown to near confluence in 6-well plates and transfected with Lipofectamine 2000 reagent (Invitrogen) in OPFull Paper
2013Patel, VM;Investigating apoptosis pathway in chronic lymphocytic leukemia: stromal influence and therapeutic activationThesisAll subjects signed written informed consent forms in accordance with the Declaration of Helsinki, and the laboratory protocols were approved by the institutional review board (IRB) at the University of Texas MD Anderson Cancer Center. Normal B‐lymphFull Paper
2013Yi, S;Green Manufacturing of Nanoparticles for Biomedical ApplicationsThesisSplenocytes, derived from C57BL/6 mice, were purchased from the Allcells Company (Emeryville, CA). HEPES, 1, 9-dimethyl-methylene blue (DMMB), chondroitin sulfate (CS), Sephadex G75, DEAE-cellulose and phosphate buffered saline (PBS) were purchased fFull Paper
2013Adams, KJ;Redirected T cell activity by high affinity TCR-ANTI-CD3 bispecific candidate therapeuticsThesis2.2.2.3 Frozen peripheral blood mononuclear cells (PBMC) derived from multiple myeloma patients PBMC derived from multiple myeloma patients were purchased from AllCells as frozen aliquots, and were thawed and used on the day of the assayFull Paper
2013PURWANTI, YIDA;CANCER IMMUNOTHERAPY: TARGETED CELLULAR VEHICLE-MEDIATED IMMUNOGENE THERAPY AND DENDRITIC CELL-BASED VACCINEThesisDCs and naïve T cells were derived from human peripheral blood mononuclear cells (PBMCs), both HLA-A2 positive and negative (AllCells, Stemcell Technologies Inc) based on previous studies 178. To generate DCs, 1×108 human PBMCs were seeded onto a T-1Full Paper
2013Kalim, KW;The role of immunoproteasome subunit LMP7 in modulating T-helper cell differentiation and progression of autoimmune diseasesThesiscells were obtained from ATCC (Manassas, VA) and cultured in media recommended by the supplier. Human peripheral blood mononuclear cells (PBMC) from normal healthy volunteers were purchased from AllCells (Emeryville, CA) and those from individuals wiFull Paper
2013Suknuntha, K;An Induced Pluripotent Stem Cell-Based Platform for In Vitro Study of Chronic Myeloid Leukemia Stem Cell Development and Drug ResistanceThesiscells from CML patients in the chronic phase were purchased commercially (AllCells or in the chronic phase were purchased commercially (AllCells or Applied StemCells), or obtained SaveFull Paper
2013O'Huallachain, ME;Genomic Variation in Somatic Human TissuesThesisfor a complexrearrangement in the PMP22 gene in an unaffected parent of a patient affect byCharcot-Marie-Tooth disease who had the same rearrangement throughout allcells. SaveFull Paper
2013Bhat, S;Impact of nicotine and PPARδ-agonist on human mesenchymal stem cellsThesisAn overview of all experiments performed in this thesis is provided on the previous page (see Figure 7). The overview includes type of experiment, treatments and assay/staining procedures conducted. Frozen (in 10%DMSO) bone marrow mononuclear cells (Full Paper
2013Kadam, S;Global Landscape of DNA Methylation and Gene Expression in Hematopoiesis and Monocytic DifferentiationThesisTable 1: Information about the samples obtained from AllCells Inc; age, sex, ethnicity, lot number, catalog number, and location of cell (Bone Marrow and Peripheral Blood). RNA Expression Analysis in the Hematopoetic Lineage Even though DNA methylatiFull Paper
2013Kohn, LA;Identification and Characterization of the Earliest Stages of Human Lymphoid CommitmentThesisTissue Core of the University of California, Los Angeles, Cincinnati Children’s Hospital, or AllCells according to guidelines approved by Institutional Review Board of the University of California, Los Angeles. Samples were enriched for CD34+ cellsFull Paper
2013Haworth, KG;Molecular Characterization of the HIV-1 Vpu Protein and Its Role in Antagonizing the Cellular Restriction Factor BST-2/Tetherin Both In Vitro and In VivoThesisHuman peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA), depleted of CD8+ T cells using a positive selection CD8 microbead isolation kit (Miltenyi Biotech, Gladbach, Germany), and cultured in supplemented R10 mediFull Paper
2013Cullimore, M;Establishing a role for IL-27 in the amelioration of MS and EAEThesisWhole venous blood was collected in EDTA-treated tubes from healthy donors after their informed consent. PBMCs were isolated using FiColl-Paque Plus™ density centrifugation while cord blood cells were obtained from AllCells. CD4+ /CD14+ cells were puFull Paper
2013Johnston, JM;Optimization of a Lentiviral Vector for use in an HSC Gene Therapy Protocol for Hemophilia AThesisCD34+ cells were purchased from AllCells (Emeryville, CA). Prior to arrival, CD34+ cells were mobilized from human bone marrow and isolated from the peripheral blood of 3 individual subjects. Flow cytometry was used to assess the purity of the sampleFull Paper
2013Xiao, L;Dendritic cell-specific vaccine utilizing antibody-mimetic ligand and lentivector systemThesisHuman PBMC-derived DCs were generated and purified as previously described,(Romani and others 1994; Sallusto and others 1994) except that human PBMCs were from HLA-A2+ patients (AllCells, LLC.). Briefly, plastic-adherent cell fractions of human PBMCsFull Paper
2013Ji, T;Lin, C;Krill, LS;Eskander, R;Guo, Y;Zi, X;Hoang, BH;Flavokawain B, a kava chalcone, inhibits growth of human osteosarcoma cells through G2/M cell cycle arrest and apoptosisMolecular cancer23764122number of 2×104 cells were mixed with FKB or Adriamycin at concentration of 8.8 nM/ml, 17.6 nM/ml and 26.4 nM/ml, respectively. The mixture was cultured in 1 ml ColonyGel 1201 Mouse Base Medium (Reachbio) using a 6-well plate under standard cultureFull Paper
2013Lee, YT;de Vasconcellos, JF;Yuan, J;Byrnes, C;Noh, SJ;Meier, ER;Kim, KS;Rabel, A;Kaushal, M;Muljo, SA;Miller, JL;LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivoBlood23798711All related studies were performed after human subjects review and National Institutes of Health Institutional Review Board approval. These studies were conducted in accordance with the Declaration of Helsinki. Human cord blood CD34+ cells were obtaiFull Paper
2013Brandao, LN;Winges, A;Christoph, S;Sather, S;Migdall-Wilson, J;Schlegel, J;McGranahan, A;Gao, D;Liang, X;Deryckere, D;Graham, DK;Inhibition of MerTK increases chemosensitivity and decreases oncogenic potential in T-cell acute lymphoblastic leukemiaBlood cancer journal23353780w cytometer and CXP data analysis software (Beckman Coulter). Methylcellulose assay Five hundred Jurkat parental and shRNA derivative cells were plated in methylcellulose-based medium (Cat no. 1101, Reachbio, Seattle, WA, USA) according to manufactuFull Paper
2013Grandi, FC;Rosser, JM;An, W;LINE-1-derived poly(A) microsatellites undergo rapid shortening and create somatic and germline mosaicism in miceMolecular biology and evolution23125228Cell and viability counts were performed using glacial acetic acid and trypan blue. Bone marrow cells were plated in ColonyGEL Complete Medium Without Epo (ReachBio LLC) at a density of 2 × 104 or 1 × 104 cells in 35 mm cell culture dishes and culturFull Paper
2013Ulyanova, T;Jiang, Y;Padilla, SM;Papayannopoulou, T;Erythroid cells generated in the absence of specific β1-integrin heterodimers accumulate reactive oxygen species at homeostasis and are unable to mount effective antioxidant defensesHaematologica23812936CFU-C assays To quantify committed progenitors of all lineages, 50,000 BM cells, 500,000 splenic cells or 50-250 microliters of peripheral blood were cultured in semi-solid methylcellulose medium supplemented with cytokines (ReachBio) for seven daysFull Paper
2013Zeiger, AS;Hinton, B;Van Vliet, KJ;Why the dish makes a difference: quantitative comparison of polystyrene culture surfacesActa biomaterialia23454055Human bone-marrow-derived mesenchymal stromal or “stem” cells (hMSCs; ReachBio Seattle, WA) were expanded until passages 4-6, and plated onto P35 TCPS dishes at low density (∼5000 cells cm−2) to maintain subconfluent culture conditionsFull Paper
2013Lee-Sherick, AB;Eisenman, KM;Sather, S;McGranahan, A;Armistead, PM;McGary, CS;Hunsucker, SA;Schlegel, J;Martinson, H;Cannon, C;Keating, AK;Earp, HS;Liang, X;DeRyckere, D;Graham, DK;Aberrant Mer receptor tyrosine kinase expression contributes to leukemogenesis in acute myeloid leukemiaOncogene23474756For methylcellulose colony forming assays, cells were plated in triplicate at a density of 500 cells/plate in Colony Gel Human Base Medium methylcellulose (Reach Bio, Seattle, WA, USA) with RPMI media containing 10% FBS added per manufacturer instrucFull Paper
2013Rossetto, CC;Tarrant-Elorza, M;Pari, GS;Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cellsPLoS pathogens23717203d, 10 ng/ml TPO, and 10 ng/ml TNF for three days, followed by the addition of 50 ng/ml lipopolysaccharide (LPS) (Sigma-Aldrich) for an additional four days. Human CD14 (+) cells purchased (Lonza and ReachBio) or isolated from cord blood were maintaiFull Paper
2013Christoph, S;Deryckere, D;Schlegel, J;Frazer, JK;Batchelor, LA;Trakhimets, AY;Sather, S;Hunter, DM;Cummings, CT;Liu, J;Yang, C;Kireev, D;Simpson, C;Norris-Drouin, J;Hull-Ryde, EA;Janzen, WP;Johnson, GL;Wang, X;Frye, SV;Earp, HS;Graham, DK;UNC569, a novel small-molecule mer inhibitor with efficacy against acute lymphoblastic leukemia in vitro and in vivoMolecular cancer therapeutics23997116Jurkat or 697 cells were plated in methylcellulose-based medium (#1101; ReachBio) containing UNC569 or DMSO control according to the manufacturer's protocol (500 cells/mL SaveFull Paper
2013Bausch, E;Kohlhof, H;Hamm, S;Krauss, R;Baumgartner, R;Sironi, L;A novel microtubule inhibitor 4SC-207 with anti-proliferative activity in taxane-resistant cellsPloS one24324550Mouse femoral bone marrow cells derived from an 8 week old Balb/c mouse were qualified at ReachBio (in terms of progenitor frequency), and stored at -152C. Cells were prepared as Save;the absorbance at time zero (drug addition), Ti the absorbance aFull Paper
2013Weerasinghe, P;Hallock, S;Brown, RE;Loose, DS;Buja, LM;A model for cardiomyocyte cell death: insights into mechanisms of oncosisExperimental and molecular pathology22609242)-derived cardiomyocytes were purchased from Reach Bio LLC (Seattle, WA) and neonatal Sanguinarine was subsequently diluted with cardiomyocyte growth media (Reach Bio LLC, SaveFull Paper
2013Maloney, JM;Lehnhardt, E;Long, AF;Van Vliet, KJ;Mechanical fluidity of fully suspended biological cellsBiophysical journal24138852Primary adult human mesenchymal stem cells (hMSCs) were isolated from the bone marrow of four adult donors via Stem Cell Technologies (Vancouver, BC, Canada), ReachBio (Seattle, WA), or Lonza Group (Basel, Switzerland)Full Paper
2013Whitfield, MJ;Lee, WC;Van Vliet, KJ;Onset of heterogeneity in culture-expanded bone marrow stromal cellsStem cell research24103495BMSCs used for long-term imaging were purchased from ReachBio, and received at passage 1 (p1). Cells were seeded at ~ 2000 cells/cm 2 on tissue culture-treated polystyrene (BD SaveFull Paper
2013Pierce, T;Keeping Piracy to a MinimumAppreneurTwo assay platforms, specifically designed for ADCs, have been developed by ReachBio as a result of increasing demand by industry for ways to assess the potential toxicity of these SaveFull Paper
2013Kennedy, JP;A cellularized biomaterial model of cardiac fibroblasts for evaluation of fibroblast induced changes in stiffnessThesisSingle donor human mesenchymal stem cells (MSC; ReachBio Seattle, WA) were passaged 2-3, and expression of CD29, CD73, CD105 CD34, and CD45 surface markers was evaluated by FACS (flow cytometry performed by manufacturer). UFull Paper
2013Rosser, JM;Investigating DNA methylation dynamics with transgenic LINE-1 mouse modelsThesisCell and viability counts were performed using glacial acetic acid and trypan blue. Bone marrow cells were plated in ColonyGEL Complete Medium Without Epo (ReachBio LLC) at a densityFull Paper
2012Xu, B;Ionita-Laza, I;Roos, JL;Boone, B;Woodrick, S;Sun, Y;Levy, S;Gogos, JA;Karayiorgou, M;De novo gene mutations highlight patterns of genetic and neural complexity in schizophreniaNat. Genet.23042115We are enormously grateful to all the families who participated in this research. We thank H. Pretorius and nursing sisters R. van Wyk, C. Botha and H. van den Berg for their assistance with subject recruitment, family history assessments and diagnosFull Paper
2012Yang, J;Ramos, E;Corces, VG;The BEAF-32 insulator coordinates genome organization and function during the evolution of Drosophila speciesGenome Res.22895281on a Branson Sonifier 250 with output control set at 1.5. Libraries were prepared with the Illumina TruSeq DNA Sample Preparation Kit and sequenced at the HudsonAlpha Institute for Biotechnology.Full Paper
2012Van Bortle, K;Ramos, E;Takenaka, N;Yang, J;Wahi, JE;Corces, VG;Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domainsGenome Res.22722341Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (NEB Cat# F-530L) and size selected for 200–300 bp by gel extraction. ChIP libraries were sequenced at the HudsonAlpha Institute for Biotechnology, using an IllFull Paper
2012Kellner, WA;Ramos, E;Van Bortle, K;Takenaka, N;Corces, VG;Genome-wide phosphoacetylation of histone H3 at Drosophila enhancers and promotersGenome Res.22508764We thank Mattias Mannervik for the gift of CBP antibody and members of the Corces Lab for invaluable discussions. We also thank The Genomic Services Lab at the HudsonAlpha Institute for Biotechnology for their help in performing Illumina sequencing oFull Paper
2012Higgins, G;Roper, KM;Watson, IJ;Blackhall, FH;Rom, WN;Pass, HI;Ainscough, JF;Coverley, D;Variant Ciz1 is a circulating biomarker for early-stage lung cancerProc. Natl. Acad. Sci. U.S.A.23074256... anti-rabbit Alexa Fluor 488 (Invitrogen, Folio Bioscience). Subcellular fractionation was carried out as described (21). For immunohistochemistry, 2B was applied to SCLC tissue microarray ARY-HH0117 (Folio Biosciences) and visualized using UltravFull Paper
2012Fabbri, M;Paone, A;Calore, F;Galli, R;Gaudio, E;Santhanam, R;Lovat, F;Fadda, P;Mao, C;Nuovo, GJ;Zanesi, N;Crawford, M;Ozer, GH;Wernicke, D;Alder, H;Caligiuri, MA;Nana-Sinkam, P;Perrotti, D;Croce, CM;MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory responseProc. Natl. Acad. Sci. U.S.A.22753494We thank Phylogeny, Inc. for performing in situ hybridization in its entirety and locked nucleic acid in situ hybridization experiments and for supporting these experiment; Dr. Cecilia FernandezCymering and Dr. Stefano Volinia for supervision of statFull Paper
2012Ren, H;Tan, ZP;Zhu, X;Crosby, K;Haack, H;Ren, JM;Beausoleil, S;Moritz, A;Innocenti, G;Rush, J;Zhang, Y;Zhou, XM;Gu, TL;Yang, YF;Comb, MJ;Identification of anaplastic lymphoma kinase as a potential therapeutic target in ovarian cancerCancer Res.22570254Histologic typing and grading were carried out according to International Federation of Gynecology and Obstetrics (FIGO) guidelines. General pathologic information of the 69 ovarian tumor patients is listed in Supplementary Table S1. FFPE ovarian tumFull Paper
2012Polisetty, RV;Gautam, P;Sharma, R;Harsha, HC;Nair, SC;Gupta, MK;Uppin, MS;Challa, S;Puligopu, AK;Ankathi, P;Purohit, AK;Chandak, GR;Pandey, A;Sirdeshmukh, R;LC-MS/MS analysis of differentially expressed glioblastoma membrane proteome reveals altered calcium signaling and other protein groups of regulatory functionsMol. Cell Proteomics22219345... tion [http://www.mcponline.org/cgi/content/full/M111.013565/DC1]. IMMUNOHISTOCHEMISTRY Formalin-fixed paraffin-embedded individual GBM tissue sections or commercially available tissue microarrays (Folio Biosciences, Columbus, OH) with 35 GBM tFull Paper
2012Gonzalez de Castro, D;Angulo, B;Gomez, B;Mair, D;Martinez, R;Suarez-Gauthier, A;Shieh, F;Velez, M;Brophy, VH;Lawrence, HJ;Lopez-Rios, F;A comparison of three methods for detecting KRAS mutations in formalin-fixed colorectal cancer specimensBr. J. Cancer22713664Materials: FFPET specimens from CRC tumours were purchased from US commercial vendors: Discovery Life Sciences, Inc. (Los Osos, CA, USA); BioServe (Beltsville, MD, USA); ProteoGenex (Culver City, CA, USA); CureLine, IncFull Paper
2012Lenehan, PF;Boardman, LA;Riegert-Johnson, D;De Petris, G;Fry, DW;Ohrnberger, J;Heyman, ER;Gerard, B;Almal, AA;Worzel, WP;Generation and external validation of a tumor-derived 5-gene prognostic signature for recurrence of lymph node-negative, invasive colorectal carcinomaCancer22605513(South San Francisco, Calif), Mayo Clinic (Rochester, Minn; Jacksonville, Fla; and Scottsdale, Ariz), Folio Biosciences (Columbus, Ohio), Trans‐Hit Biomarkers, Inc. (Montreal, Quebec, Canada), BioServe (Laurel, Md), and Amp‐Tec (Hamburg, Germany)Full Paper
2012Arrington, CB;Bleyl, SB;Matsunami, N;Bonnell, GD;Otterud, BE;Nielsen, DC;Stevens, J;Levy, S;Leppert, MF;Bowles, NE;Exome analysis of a family with pleiotropic congenital heart diseaseCirc Cardiovasc Genet22337856Exome sequencing (library construction, capture and sequencing) was carried out on 2 DNA samples (from patients III.3 and III.7) at the genomic service laboratory at HudsonAlpha Institute for Biotechnology (Huntsville, Alabama). Briefly, 3 to 5 µg ofFull Paper
2012Dahmen, S;Haenni, M;Madec, JY;IncI1/ST3 plasmids contribute to the dissemination of the blaCTX-M-1 gene in Escherichia coli from several animal species in FranceJ. Antimicrob. Chemother.22872449Characteristics of the eight CTX-M-1-producing E. coli isolates described in this study Strain Year District Animal Pathology b-Lactamases Additional resistancesa Phylogeny CTX-M-1 plasmid Inc group sequence type size (kb) 16170 2006 76 dog diarrhoeaFull Paper
2012Fedyk, ER;Wyant, T;Yang, LL;Csizmadia, V;Burke, K;Yang, H;Kadambi, VJ;Exclusive antagonism of the α4 β7 integrin by vedolizumab confirms the gut-selectivity of this pathway in primatesInflamm. Bowel Dis.22419649Immunohistochemistry detecting the β7 integrin was conducted using a sheep antihuman β7 polyclonal antibody (R&D Systems, Minneapolis, MN) that does not compete with vedolizumab for binding to the α4β7 integrin expressed by leukocytes (data not shownFull Paper
2012Jenny, MJ;Aluru, N;Hahn, ME;Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryosToxicol. Appl. Pharmacol.22921993The same total RNA used for the array hybridizations was also used for high-throughput sequencing using the SOLiD technology. Prior to sequencing, the quality of the RNA and presence of the microRNA fraction was confirmed with the Agilent Small RNA kFull Paper
2012Liu, S;Zhang, Y;Zhou, Z;Waldbieser, G;Sun, F;Lu, J;Zhang, J;Jiang, Y;Zhang, H;Wang, X;Rajendran, KV;Khoo, L;Kucuktas, H;Peatman, E;Liu, Z;Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygoteBMC Genomics23127152;... urer's protocol as in previous study [5]. Equal amount of total RNA from each tissue was combined and sent out for commercial sequencing. Illumina sequencing Sequencing was conducted commercially in HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2012Rokyta, DR;Lemmon, AR;Margres, MJ;Aronow, K;The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus)BMC Genomics23025625Sequencing and nonnormalized cDNA library preparation were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially as described by MortFull Paper
2012Sun, F;Peatman, E;Li, C;Liu, S;Jiang, Y;Zhou, Z;Liu, Z;Transcriptomic signatures of attachment, NF-κB suppression and IFN stimulation in the catfish gill following columnaris bacterial infectionDev. Comp. Immunol.; for use in RNA-seq. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) as previously described by Li et al. (2012). cDNA libraries were prepared withFull Paper
2012Li, C;Zhang, Y;Wang, R;Lu, J;Nandi, S;Mohanty, S;Terhune, J;Liu, Z;Peatman, E;RNA-seq analysis of mucosal immune responses reveals signatures of intestinal barrier disruption and pathogen entry following Edwardsiella ictaluri infection in channel catfish, Ictalurus punctatusFish Shellfish Immunol.22366064; replicate control pool was formed for use in RNA-seq. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25 ug ofFull Paper
2012Nguyen, TH;Weber, W;Havari, E;Connors, T;Bagley, RG;McLaren, R;Nambiar, PR;Madden, SL;Teicher, BA;Roberts, B;Kaplan, J;Shankara, S;Expression of TMPRSS4 in non-small cell lung cancer and its modulation by hypoxiaInt. J. Oncol.22692880Immunohistochemistry. A tissue array containing 101 formalin-fixed, paraffin-embedded tissue cores of lung SCC, adenocarcinomas, and normal non-neoplastic lung tissues from multiple donors was used (Phylogeny Inc., Columbus, OH)Full Paper
2012Gurudatta, BV;Ramos, E;Corces, VG;The BEAF insulator regulates genes involved in cell polarity and neoplastic growthDev. Biol.22743648ChIP libraries were sequenced at the HudsonAlpha Institute for Biotechnology, using an Illumina HiSeq system. Sequences were mapped to the dm3 genome with Bowtie 0.12.3 (Langmead et al., 2009) using default settings. Peaks were then called with MACSFull Paper
2012Lee, S;Brophy, VH;Cao, J;Velez, M;Hoeppner, C;Soviero, S;Lawrence, HJ;Analytical performance of a PCR assay for the detection of KRAS mutations (codons 12/13 and 61) in formalin-fixed paraffin-embedded tissue samples of colorectal carcinomaVirchows Arch.22173329Material and methods. Materials. FFPET specimens from CRC tumors were purchased from US commercial vendors: Discovery Life Sciences, Inc. (Los Osos, CA, USA); BioServe (Beltsville, MD, USA); ProteoGenex (Culver City, CA, USA); CureLine, IncFull Paper
2012Hughes, JA;Lynch, FC;Transhepatic removal of an inferior vena cava filterJ Vasc Interv Radiol22720899Full Paper
2012Lynch, FC;Modified loop snare technique for the removal of bard recovery, G2, G2 express, and eclipse inferior vena cava filtersJ Vasc Interv Radiol22525025Full Paper
2012Vijay, K;Hughes, JA;Burdette, AS;Scorza, LB;Singh, H;Waybill, PN;Lynch, FC;Fractured Bard Recovery, G2, and G2 express inferior vena cava filters: incidence, clinical consequences, and outcomes of removal attemptsJ Vasc Interv Radiol22173108Full Paper
2012Mutka, SC;Green, LH;Verderber, EL;Richards, JP;Looker, DL;Chlipala, EA;Rosenthal, GJ;ADH IB expression, but not ADH III, is decreased in human lung cancerPLoS ONE23285246GSNOR immunohistochemistry. Immunohistochemical analysis for GSNOR was performed using formalin-fixed, paraffin-embedded human lung tissue microarrays (Folio Bioscience, Columbus, OH; #ARY-HH0178, #ARY-HH0176)Full Paper
2012Ueki, A;Shimizu, T;Masuda, K;Yamaguchi, SI;Ishikawa, T;Sugihara, E;Onishi, N;Kuninaka, S;Miyoshi, K;Muto, A;Toyama, Y;Banno, K;Aoki, D;Saya, H;Up-regulation of Imp3 confers in vivo tumorigenicity on murine osteosarcoma cellsPLoS ONE23226335Zeiss). Human Osteosarcoma Tissue Array An array of human osteosarcoma specimens was obtained from Folio Biosciences (Powell, OH) and was subjected to immunohis- tochemical staining for IMP3 as described above ; Human Osteosarcoma Tissue Array.Full Paper
2012Xu, J;Ji, P;Zhao, Z;Zhang, Y;Feng, J;Wang, J;Li, J;Zhang, X;Zhao, L;Liu, G;Xu, P;Sun, X;Genome-wide SNP discovery from transcriptome of four common carp strainsPLoS ONE23110192;... e distribution of all samples were checked with Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, USA). cDNA Library Construction Illumina Sequencing Sequencing of 24 samples was conducted in HudsonAlpha Genomic Services Laboratory (HuntFull Paper
2012Hedin, M;Starrett, J;Akhter, S;Schönhofer, AL;Shultz, JW;Phylogenomic resolution of paleozoic divergences in harvestmen (Arachnida, Opiliones) via analysis of next-generation transcriptome dataPLoS ONE22936998Protolophus total RNA was shipped to Cofactor Genomics (www.cofactorgenomics.com), where a duplex-specific nuclease (DSN) normalized library was constructed, and 60 basepair (bp) paired-end reads were generated using Illumina GAII technology. All othFull Paper
2012Johansson, S;Irgens, H;Chudasama, KK;Molnes, J;Aerts, J;Roque, FS;Jonassen, I;Levy, S;Lima, K;Knappskog, PM;Bell, GI;Molven, A;Njølstad, PR;Exome sequencing and genetic testing for MODYPLoS ONE22662265Targeted capture and massive parallel sequencing were performed at HudsonAlpha Institute for Biotechnology (Huntsville, AL) (File S1). In brief, SureSelect Human All Exon Kit (Agilent Technologies, Santa Clara, CA) was used for exome enrichment, andFull Paper
2012Myers, RB;Rwayitare, K;Richey, L;Lem, J;Castellot, JJ;CCN5 Expression in mammals. III. Early embryonic mouse developmentJ Cell Commun Signal22926930earlier, at the 4-cell stage. Materials and methods. Immunohistochemistry. 5 μm frozen sagittal sections of C57BL/6 post-implantation embryos were obtained from Folio Bio (Columbus, OH). Sections were cleared with xyleneFull Paper
2012Ding, D;Scott, NM;Thompson, EE;Chaiworapongsa, T;Torres, R;Billstrand, C;Murray, K;Dexheimer, PJ;Ismail, M;Kay, H;Levy, S;Romero, R;Lindheimer, MD;Nicolae, DL;Ober, C;Increased protein-coding mutations in the mitochondrial genome of African American women with preeclampsiaReprod Sci22902742Mitochondrial genome libraries were prepared by amplification of the complete mtDNA genome using 2 sets of overlapping primers. These fragments were pooled in molar equivalent amounts, sonicated to randomly fragment the DNA, and then prepared for seqFull Paper
2012Morimoto, M;Souich, Cd;Trinh, J;McLarren, KW;Boerkoel, CF;Hendson, G;Expression profile of NSDHL in human peripheral tissuesJ. Mol. Histol.22113624This construct was cut at unique NotI and SpeI sites flanking the Nsdhl insert and transcribed by T3 and T7 RNA polymerases to yield antisense and sense in situ hybridization probes, respectively. In situ hybridization was performed by Phylogeny, IncFull Paper
2012Wang, R;Li, C;Stoeckel, J;Moyer, G;Liu, Z;Peatman, E;Rapid development of molecular resources for a freshwater mussel, Villosa lienosa (Bivalvia:Unionidae), using an RNA-seq-based approachFreshwater ScienceAfter quantitation with KAPA Library Quant Kits (Kapa Biosystems, Woburn, Massachusetts) and dilution, the libraries were clustered 4 per lane and sequenced on a HiSeq 2000 instrument with 100-bp PE reads at the HudsonAlpha Genomic Services LaboratorFull Paper
2012Kishida, M;Ishige, K;Horibe, T;Tada, N;Koibuchi, N;Shoda, J;Kita, K;Kawakami, K;Orexin 2 receptor as a potential target for immunotoxin and antibody-drug conjugate cancer therapyOncol Lett22740944Materials and methods. Materials. Hepatocellular carcinoma tissue array, ARY- HH0075 was purchased from Folio Biosciences (Columbus, OH, USA). Digestive system disease tissue array, DID381 was purchased from US Biomax (Rockville, MD, USA)Full Paper
2012Kentsis, A;Reed, C;Rice, KL;Sanda, T;Rodig, SJ;Tholouli, E;Christie, A;Valk, PJ;Delwel, R;Ngo, V;Kutok, JL;Dahlberg, SE;Moreau, LA;Byers, RJ;Christensen, JG;Vande Woude, G;Licht, JD;Kung, AL;Staudt, LM;Look, AT;Autocrine activation of the MET receptor tyrosine kinase in acute myeloid leukemiaNature medicine22683780This report identifies upregulation of HGF as an autocrine growth pathway in several subsets of AML. Ligand-dependent activation of MET represents a new oncogenic stimulus, and the SaveFull Paper
2012Schenk, T;Chen, WC;Göllner, S;Howell, L;Jin, L;Hebestreit, K;Klein, HU;Popescu, AC;Burnett, A;Mills, K;Casero, RA;Marton, L;Woster, P;Minden, MD;Dugas, M;Wang, JC;Dick, JE;Müller-Tidow, C;Petrie, K;Zelent, A;Inhibition of the LSD1 (KDM1A) demethylase reactivates the all-trans-retinoic acid differentiation pathway in acute myeloid leukemiaNature medicine22406747We grew normal bone marrow mononuclear cells (AllCells, ABM011F) in IMDM supplemented with 20 ng ml −1 SCF, 10 ng ml −1 each of IL-3, IL-6 and fms-related tyrosine kinase 3 ( SaveFull Paper
2012Zhang, C;Srinivasan, Y;Arlow, DH;Fung, JJ;Palmer, D;Zheng, Y;Green, HF;Pandey, A;Dror, RO;Shaw, DE;Weis, WI;Coughlin, SR;Kobilka, BK;High-resolution crystal structure of human protease-activated receptor 1Nature23222541In brief, acid-citrate-dextrose anti-coagulated human blood samples (60 ml per donor) were obtained from AllCells. Blood was centrifuged without braking at 250g at 37 C for 15 min. SaveFull Paper
2012Sasaki, M;Knobbe, CB;Munger, JC;Lind, EF;Brenner, D;Brüstle, A;Harris, IS;Holmes, R;Wakeham, A;Haight, J;You-Ten, A;Li, WY;Schalm, S;Su, SM;Virtanen, C;Reifenberger, G;Ohashi, PS;Barber, DL;Figueroa, ME;Melnick, A;Zúñiga-Pflücker, JC;Mak, TW;IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigeneticsNature22763442Two normal CD34 + bone marrow control samples were purchased from AllCells. Institutional review board approval was obtained at Weill Cornell Medical Center and this study was SaveFull Paper
2012Kohn, LA;Hao, QL;Sasidharan, R;Parekh, C;Ge, S;Zhu, Y;Mikkola, HK;Crooks, GM;Lymphoid priming in human bone marrow begins before expression of CD10 with upregulation of L-selectinNature immunology22941246esis in disease states. METHODS Bone marrow cell isolation Normal human BM and thymic cells were obtained from healthy donors via the UCLA Pathology Tissue Core, Cincinnati Children’s Hospital, or ALLCELLS according to guidelines approved by UCLA InFull Paper
2012Zhu, X;Wang, Y;Pi, W;Liu, H;Wickrema, A;Tuan, D;NF-Y recruits both transcription activator and repressor to modulate tissue- and developmental stage-specific expression of human γ-globin genePloS one23071749The human cells were obtained commercially from AllCells and processed with protocols approved by Human Assurance Committee of Georgia Health Sciences University (HAC file # SaveFull Paper
2012Hur, EM;Patel, SN;Shimizu, S;Rao, DS;Gnanapragasam, PN;An, DS;Yang, L;Baltimore, D;Inhibitory effect of HIV-specific neutralizing IgA on mucosal transmission of HIV in humanized miceBlood23065154intraperitoneally with 3 × 10 6 human PBMCs (AllCells) that were activated by PHA (5 μg/mL) NSG mice were transplanted intraperitoneally with 3 × 10 6 human PBMCs (AllCells) that SaveFull Paper
2012Lu, M;Wang, X;Li, Y;Tripodi, J;Mosoyan, G;Mascarenhas, J;Kremyanskaya, M;Najfeld, V;Hoffman, R;Combination treatment in vitro with Nutlin, a small-molecule antagonist of MDM2, and pegylated interferon-α 2a specifically targets JAK2V617F-positive polycythemia vera cellsBlood22872685Fresh normal human bone marrow CD34+ cells were purchased from ALLCELLS. The characteristics of the PV patients studied are shown in supplemental Table 1 (available on the Blood Web site; see the Supplemental Materials link at the top of the online aFull Paper
2012Ndhlovu, LC;Lopez-Vergès, S;Barbour, JD;Jones, RB;Jha, AR;Long, BR;Schoeffler, EC;Fujita, T;Nixon, DF;Lanier, LL;Tim-3 marks human natural killer cell maturation and suppresses cell-mediated cytotoxicityBlood22383801Peripheral blood mononuclear cells (PBMCs) of healthy individuals were obtained from the Stanford Blood Bank. Cord blood PMBCs were obtained from AllCells. The University of California, San Francisco and the University of Hawaii institutional reviewFull Paper
2012Wey, S;Luo, B;Tseng, CC;Ni, M;Zhou, H;Fu, Y;Bhojwani, D;Carroll, WL;Lee, AS;Inducible knockout of GRP78/BiP in the hematopoietic system suppresses Pten-null leukemogenesis and AKT oncogenic signalingBlood21937694The normal peripheral blood mononuclear cells were purchased from ALLCELLS (catalog #PB003F). The ethical use of the human tissues for research was approved by the University SaveFull Paper
2012Cheng, L;Hansen, NF;Zhao, L;Du, Y;Zou, C;Donovan, FX;Chou, BK;Zhou, G;Li, S;Dowey, SN;Ye, Z;NISC Comparative Sequencing Program, ;Chandrasekharappa, SC;Yang, H;Mullikin, JC;Liu, PP;Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by nonintegrating plasmid expressionCell stem cell22385660blood of anonymous donors were collected and processed at AllCells, LLC (Emeryville, CA). The by Institutional Review Board (IRB) at AllCells. Use of anonymous human samples for SaveFull Paper
2012Smith, MR;Atkinson, P;White, D;Piersma, T;Gutierrez, G;Rossini, G;Desai, S;Wellinghoff, S;Yu, H;Cheng, X;Design and assessment of a wrapped cylindrical Ca-P AZ31 Mg alloy for critical-size ulna defect repairJournal of biomedical materials research. Part B, Applied biomaterials22102519AZ31 Mg alloy cupons were prepared as described above and sterilized using ethylene oxide gas. Two cell migration tests were performed: indirect and direct. Frozen human MSCs (AllCells, LLC, Emeryville, CA) were thawed, spun down, and resuspended inFull Paper
2012Muthuswamy, R;Berk, E;Junecko, BF;Zeh, HJ;Zureikat, AH;Normolle, D;Luong, TM;Reinhart, TA;Bartlett, DL;Kalinski, P;NF-κB hyperactivation in tumor tissues allows tumor-selective reprogramming of the chemokine microenvironment to enhance the recruitment of cytolytic T effector cellsCancer research22593190vein endothelial cells (HUVEC; AllCells) were cultured in HUVEC complete media (Basal media supplemented with HUVEC stimulatory supplement; AllCells). All cell lines were washed SaveFull Paper
2012Capozza, F;Trimmer, C;Castello-Cros, R;Katiyar, S;Whitaker-Menezes, D;Follenzi, A;Crosariol, M;Llaverias, G;Sotgia, F;Pestell, RG;Lisanti, MP;Genetic ablation of Cav1 differentially affects melanoma tumor growth and metastasis in mice: role of Cav1 in Shh heterotypic signaling and transendothelial migrationCancer research22396494B16F10 and A-375 were from ATCC, while human immortalized dermal foreskin fibroblasts (hTBJ1) were originally from Clontech. Human Umbilical Vein Endothelial Cells (HUVEC) were purchased from ALLCELLS. Only early passages of these cell lines were useFull Paper
2012Silverman, AM;Nakata, R;Shimada, H;Sposto, R;DeClerck, YA;A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastomaCancer research22389450Interleukin-6 (IL-6) is a pleiotropic cytokine with a broad range of physiologic and pathologic functions. Because in cancer, IL-6 contributes to a microenvironment that promotes tumor SaveFull Paper
2012Pacienza, N;Yoshimitsu, M;Mizue, N;Au, BC;Wang, JC;Fan, X;Takenaka, T;Medin, JA;Lentivector transduction improves outcomes over transplantation of human HSCs alone in NOD/SCID/Fabry miceMolecular therapy : the journal of the American Society of Gene Therapy22472949Human mobilized PB CD34+ cells were obtained from AllCells (Emeryville, CA). These cells were rapidly thawed following the manufacturer's recommendations. After thawing, ~85% of the cells were viable as assessed by trypan blue exclusion, while the hCFull Paper
2012Lee, EJ;Choi, EK;Kang, SK;Kim, GH;Park, JY;Kang, HJ;Lee, SW;Kim, KH;Kwon, JS;Lee, KH;Ahn, Y;Lee, HJ;Cho, HJ;Choi, SJ;Oh, WI;Park, YB;Kim, HS;N-cadherin determines individual variations in the therapeutic efficacy of human umbilical cord blood-derived mesenchymal stem cells in a rat model of myocardial infarctionMolecular therapy : the journal of the American Society of Gene Therapy22068423PBMCs (1 × 105/well) or hUCB-MSCs (1 × 102, 1 × 103 or 1 × 104/well) in 96-well tissue culture plates. The PBMCs were purchased from AllCells (Emeryville, CA) The stimulator PBMCs and hUCB-MSCs were inactivated by treatment with 10 µg/ml mitomycin-CFull Paper
2012Goh, KC;Novotny-Diermayr, V;Hart, S;Ong, LC;Loh, YK;Cheong, A;Tan, YC;Hu, C;Jayaraman, R;William, AD;Sun, ET;Dymock, BW;Ong, KH;Ethirajulu, K;Burrows, F;Wood, JM;TG02, a novel oral multi-kinase inhibitor of CDKs, JAK2 and FLT3 with potent anti-leukemic propertiesLeukemia21860433Primary cells—either peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients—were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex ( SaveFull Paper
2012Boitano, AE;de Lichtervelde, L;Snead, JL;Cooke, MP;Schultz, PG;An image-based screen identifies a small molecule regulator of megakaryopoiesisProceedings of the National Academy of Sciences of the United States of America22891346Human CD34+ cells were purified from fresh human leukophoresed-mobilized peripheral blood (AllCells) or from umbilical-cord blood (Bioreclamation), as noted, using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following manufacturer'sFull Paper
2012Kobune, M;Iyama, S;Kikuchi, S;Horiguchi, H;Sato, T;Murase, K;Kawano, Y;Takada, K;Ono, K;Kamihara, Y;Hayashi, T;Miyanishi, K;Sato, Y;Takimoto, R;Kato, J;Stromal cells expressing hedgehog-interacting protein regulate the proliferation of myeloid neoplasmsBlood cancer journal22961059Minneapolis, MN, USA). 5-Aza-2′-deoxycytidine (5-aza-dC) was obtained from Sigma (St Louis, MO, USA). Total RNA derived from mobilized peripheral blood (PB), BM or CB CD34+ cells was purchased from AllCells, LLC (Toronto, Ontario, Canada). Human myelFull Paper
2012Novotny-Diermayr, V;Hart, S;Goh, KC;Cheong, A;Ong, LC;Hentze, H;Pasha, MK;Jayaraman, R;Ethirajulu, K;Wood, JM;The oral HDAC inhibitor pracinostat (SB939) is efficacious and synergistic with the JAK2 inhibitor pacritinib (SB1518) in preclinical models of AMLBlood cancer journal22829971Peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex (Culver City, CA, USA) SaveFull Paper
2012Gao, N;Cheng, S;Budhraja, A;Gao, Z;Chen, J;Liu, EH;Huang, C;Chen, D;Yang, Z;Liu, Q;Li, P;Shi, X;Zhang, Z;Ursolic acid induces apoptosis in human leukaemia cells and exhibits anti-leukaemic activity in nude mice through the PKB pathwayBritish journal of pharmacology21950524Blasts were suspended at 8 × 105 mL-1 and incubated in RPMI 1640 medium containing 10% FBS in 24-well plates. Fresh normal bone marrow mononuclear cells were purchased from Allcells (Emeryvill, CA). After being washed and counted, cells were suspendeFull Paper
2012De Giovanni, C;Nicoletti, G;Landuzzi, L;Romani, F;Croci, S;Palladini, A;Murgo, A;Antognoli, A;Ianzano, ML;Stivani, V;Grosso, V;Iezzi, M;Stramucci, L;Barbieri, E;Lemoli, RM;Nanni, P;Lollini, PL;Human responses against HER-2-positive cancer cells in human immune system-engrafted miceBritish journal of cancer22929887ec, Bergisch Gladbach, Germany) to obtain highly purified cells (mean purity 93%±6%) as previously described (Rossi et al, 2007). For challenge experiments, CD34+ or CD133+ cells were purchased from AllCells (Emeryville, CA, USA). All the experimentsFull Paper
2012Fukushima, H;Kawanabe, N;Murata, S;Ishihara, Y;Yanagita, T;Balam, TA;Yamashiro, T;SSEA-4 is a marker of human deciduous periodontal ligament stem cellsJournal of dental research22895512Human bone marrow cells were purchased from AllCells (Emeryville, CA, USA) and were maintained in low-glucose DMEM supplemented with 10% HIFBS and penicillin/ streptomycin. SaveFull Paper
2012Kim, J;Denu, RA;Dollar, BA;Escalante, LE;Kuether, JP;Callander, NS;Asimakopoulos, F;Hematti, P;Macrophages and mesenchymal stromal cells support survival and proliferation of multiple myeloma cellsBritish journal of haematology22583117Bone marrow (BM) microenvironment provides crucial supports to normal and malignant hematopoiesis and several types of cells such as endothelial cells, osteoblasts and SaveFull Paper
2012Cokic, VP;Bhattacharya, B;Beleslin-Cokic, BB;Noguchi, CT;Puri, RK;Schechter, AN;JAK-STAT and AKT pathway-coupled genes in erythroid progenitor cells through ontogenyJournal of translational medicine22676255mmunomagnetic selection with MACS cell isolation system (Miltenyi Biotec, Auburn, CA). Commercial FL-derived CD34+ cells (Cambrex Bio Science, Inc., Walkersville, MD), CB- and BM-derived CD34+ cells (AllCells LLC, Berkeley, CA) are also isolated by pFull Paper
2012Mao, M;Sudhahar, V;Ansenberger-Fricano, K;Fernandes, DC;Tanaka, LY;Fukai, T;Laurindo, FR;Mason, RP;Vasquez-Vivar, J;Minshall, RD;Stadler, K;Bonini, MG;Nitroglycerin drives endothelial nitric oxide synthase activation via the phosphatidylinositol 3-kinase/protein kinase B pathwayFree radical biology & medicine22037515Human and bovine aortic endothelial cells (HAEC or BAEC; Allcells, Emeryville, CA, USA), human microvascular endothelial cells (HMEC), and primary mouse endothelial cells (MEC) were cultured at 37 °C, 5% CO2 in corresponding endothelial medium with sFull Paper
2012William, AD;Lee, AC;Poulsen, A;Goh, KC;Madan, B;Hart, S;Tan, E;Wang, H;Nagaraj, H;Chen, D;Lee, CP;Sun, ET;Jayaraman, R;Pasha, MK;Ethirajulu, K;Wood, JM;Dymock, BW;Discovery of the macrocycle (9E)-15-(2-(pyrrolidin-1-yl)ethoxy)-7,12,25-trioxa-19,21,24-triaza-tetracyclo[18.3.1.1(2,5).1(14,18)]hexacosa-1(24),2,4,9,14(26),15,17,20,22-nonaene (SB1578), a potent inhibitor of janus kinase 2/fms-like tyrosine kinase-3 (JAK2/FLT3) for the treatment of rheumatoid arthritisJournal of medicinal chemistry22339472These cells were cultured as described previously. (34) Human CD4+ T cells, isolated from peripheral blood mononuclear cells with 95% purity, were purchased from AllCells (Emeryville, CA) and maintained in X-VIVO 20 media (Lonza, Basel, Switzerland)Full Paper
2012Tran, CA;Torres-Coronado, M;Gardner, A;Gu, A;Vu, H;Rao, A;Cao, LF;Ahmed, A;Digiusto, D;Optimized processing of growth factor mobilized peripheral blood CD34+ products by counterflow centrifugal elutriationStem cells translational medicine23197821HPC-A products were obtained from AllCells LLC (Emeryville, CA, http://www.allcells.com), Key Biologics LLC (Memphis, TN, http://www.keybiologics.com), or Progenitor Cell Therapy LLC (Mountain View, CA, http://www.pctcelltherapy.com). HPC-A productsFull Paper
2012Rodriguez-Menocal, L;Salgado, M;Ford, D;Van Badiavas, E;Stimulation of skin and wound fibroblast migration by mesenchymal stem cells derived from normal donors and chronic wound patientsStem cells translational medicine23197781We isolated and expanded human MSCs from fresh whole bone marrow obtained from normal donors and chronic wound patients. Normal donor bone marrow was acquired from AllCells LLC (Emeryville, CA, http://www.allcells.com). Chronic wound patient bone marFull Paper
2012Oskouei, BN;Lamirault, G;Joseph, C;Treuer, AV;Landa, S;Da Silva, J;Hatzistergos, K;Dauer, M;Balkan, W;McNiece, I;Hare, JM;Increased potency of cardiac stem cells compared with bone marrow mesenchymal stem cells in cardiac repairStem cells translational medicine23197758Human bone marrow aspirates were obtained from AllCells LLC (Emeryville, CA, http://allcells.com). Low-density mononuclear cells were separated from human bone marrow aspirates and cultured as described (see supplemental online data). Adherent MSCs wFull Paper
2012Soler, R;Füllhase, C;Hanson, A;Campeau, L;Santos, C;Andersson, KE;Stem cell therapy ameliorates bladder dysfunction in an animal model of Parkinson diseaseThe Journal of urology22341818Different cell based therapies have been tested, focusing on motor function. We evaluated the effect of human amniotic fluid stem cells and bone marrow derived mesenchymal stem cells (ALLCELLS, Emeryville, California) on bladder dysfunction in a ratFull Paper
2012Hirata, Y;Ikeda, K;Sudoh, M;Tokunaga, Y;Suzuki, A;Weng, L;Ohta, M;Tobita, Y;Okano, K;Ozeki, K;Kawasaki, K;Tsukuda, T;Katsume, A;Aoki, Y;Umehara, T;Sekiguchi, S;Toyoda, T;Shimotohno, K;Soga, T;Nishijima, M;Taguchi, R;Kohara, M;Self-enhancement of hepatitis C virus replication by promotion of specific sphingolipid biosynthesisPLoS pathogens22916015les containing 200 µg protein were assayed for SPT activity using [14C]-serine and palmitoyl-CoA (Sigma-Aldrich, St. Louis, MO, USA) as substrates. Proliferation assay Human peripheral blood cells (AllCells, Emeryville, CA, USA) were plated onto 96-Full Paper
2012Köllmer, M;Keskar, V;Hauk, TG;Collins, JM;Russell, B;Gemeinhart, RA;Stem cell-derived extracellular matrix enables survival and multilineage differentiation within superporous hydrogelsBiomacromolecules22404228Human bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque PLUS solution and RosetteSep SaveFull Paper
2012Sharma, SM;Fuchsluger, T;Ahmad, S;Katikireddy, KR;Armant, M;Dana, R;Jurkunas, UV;Comparative analysis of human-derived feeder layers with 3T3 fibroblasts for the ex vivo expansion of human limbal and oral epitheliumStem cell reviews and reports21964568Bone marrow samples (3-10 ml) were purchased from AllCells (Emeryville, CA) and plated in T175 flasks in growth media consisting of α-MEM (Lonza, Walkersville, MD) supplemented SaveFull Paper
2012McNamara, LA;Ganesh, JA;Collins, KL;Latent HIV-1 infection occurs in multiple subsets of hematopoietic progenitor cells and is reversed by NF-κB activationJournal of virology22718820bone marrow (BM) was obtained commercially (AllCells Ltd.); mononuclear cells were purified Presorted CD133 + BM or CB cells were obtained commercially (AllCells Ltd.) and were SaveFull Paper
2012Yu, X;McCarthy, PJ;Wang, Z;Gorlen, DA;Mertz, JE;Shutoff of BZLF1 gene expression is necessary for immortalization of primary B cells by Epstein-Barr virusJournal of virology22623769B-cell proliferation assay. Human primary blood B cells were purchased from AllCells (Emeryville, CA), infected with the indicated amount of virus in green Raji units (GRU)/cell, aliquoted at 1 × 104 cells per well in 96-well plates, incubated at 37°Full Paper
2012Nan, XW;Tang, GJ;Xu, JG;Peng, TS;Miao, LS;Xu, ZG;Bai, C;Yu, P;Gao, YL;Hao, BT;Imaging manifestations of renal primary neuroectodermal tumorsChinese medical journal23044335Pathologicaland jmm unohistochemicalfindingsshowed portionofthetum ornecrosedanduni—morp hologicaloval orround sm allcells with rosettes structure underlight mi croscope.Im SaveFull Paper
2012Lin, W;Oh, SK;Choo, AB;George, AJ;Activated T cells modulate immunosuppression by embryonic-and bone marrow-derived mesenchymal stromal cells through a feedback mechanismCytotherapy22136295Frozen peripheral blood mononuclear cells (PBMC) were purchased from Allcells (Emeryville, CA, USA). All isolated T-cell cultures were maintained in Roswell Park Memorial Institute (RPMI)-1640 supplemented with 10% FBS, 2 mm l-glutamine, 50 U/mL peniFull Paper
2012Akalin, A;Garrett-Bakelman, FE;Kormaksson, M;Busuttil, J;Zhang, L;Khrebtukova, I;Milne, TA;Huang, Y;Biswas, D;Hess, JL;Allis, CD;Roeder, RG;Valk, PJ;Löwenberg, B;Delwel, R;Fernandez, HF;Paietta, E;Tallman, MS;Schroth, GP;Mason, CE;Melnick, A;Figueroa, ME;Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemiaPLoS genetics22737091kemia gene rearranged AML samples harboring t(9;11) (MLL1 and MLL2) and one additional AML sample (AML) were available for processing. Two normal CD34+ bone marrow control samples were purchased from AllCells, LLC (Emeryville, CA, USA). InstitutionalFull Paper
2012Chaudhry, P;Yang, X;Wagner, M;Jong, A;Wu, L;Retinoid-regulated FGF8f secretion by osteoblasts bypasses retinoid stimuli to mediate granulocytic differentiation of myeloid leukemia cellsMolecular cancer therapeutics22135230Normal human primitive hematopoietic CD34 + cells were obtained from AllCells and CD34 cells, certified to be HIV- and Mycoplasma-free by AllCells, were cultured for maxi SaveFull Paper
2012Budhraja, A;Gao, N;Zhang, Z;Son, YO;Cheng, S;Wang, X;Ding, S;Hitron, A;Chen, G;Luo, J;Shi, X;Apigenin induces apoptosis in human leukemia cells and exhibits anti-leukemic activity in vivoMolecular cancer therapeutics22084167U937, Jurkat, and HL60 human leukemia cells were obtained from American Type Culture Collection and grown in RPMI-1640 medium with 10% FBS. Normal peripheral blood mononuclear cells (NPBMNC) were obtained from AllCells and maintained in RPMI-1640 witFull Paper
2012de Lichtervelde, L;Antal, CE;Boitano, AE;Wang, Y;Krastel, P;Petersen, F;Newton, AC;Cooke, MP;Schultz, PG;Euphohelioscopin A is a PKC activator capable of inducing macrophage differentiationChemistry & biology22921066R & D Systems]) unless otherwise indicated. Human mPB CD34+ cells were purified from fresh human leukophoresed mobilized peripheral blood (AllCells) using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following manufacturer’s protocolsFull Paper
2012Qu, X;Zhuang, G;Yu, L;Meng, G;Ferrara, N;Induction of Bv8 expression by granulocyte colony-stimulating factor in CD11b+Gr1+ cells: key role of Stat3 signalingThe Journal of biological chemistry22528488Freshly isolated bone marrow cells from five different healthy donors were obtained from AllCells (Emeryville, CA). Red blood cells were lysed by adding 4 volumes of 1× buffered SaveFull Paper
2012Rowe, DD;Leonardo, CC;Recio, JA;Collier, LA;Willing, AE;Pennypacker, KR;Human umbilical cord blood cells protect oligodendrocytes from brain ischemia through Akt signal transductionThe Journal of biological chemistry22158864Human umbilical cord blood (HUCB) cells protect the brain against ischemic injury, yet the mechanism of protection remains unclear. Using both in vitro and in vivo paradigms, this SaveFull Paper
2012Madan, B;Goh, KC;Hart, S;William, AD;Jayaraman, R;Ethirajulu, K;Dymock, BW;Wood, JM;SB1578, a novel inhibitor of JAK2, FLT3, and c-Fms for the treatment of rheumatoid arthritisJournal of immunology (Baltimore, Md. : 1950)22962687Cells CD4+ T cells isolated from human PBMCs, with 95% purity were obtained from AllCells (Emeryville, CA). The THP-1 cell line was purchased from American Type Culture Collection and maintained as recommendedFull Paper
2012Liu, W;Yan, M;Liu, Y;McLeish, KR;Coleman, WG;Rodgers, GP;Olfactomedin 4 inhibits cathepsin C-mediated protease activities, thereby modulating neutrophil killing of Staphylococcus aureus and Escherichia coli in miceJournal of immunology (Baltimore, Md. : 1950)22844115Human fresh bone-marrow neutrophils were obtained from AllCells (Emeryville, CA). Mouse neutrophils were isolated from bone marrow. The femur was removed from mice, then SaveFull Paper
2012Ohtsubo, S;Ishikawa, M;Kamei, N;Kijima, Y;Suzuki, O;Sunagawa, T;Higashi, Y;Masuda, H;Asahara, T;Ochi, M;The therapeutic potential of ex vivo expanded CD133+ cells derived from human peripheral blood for peripheral nerve injuriesJournal of neurosurgery22880720We used granulocyte-colony stimulating factor-mobilized CD133+ cells derived from human peripheral blood (lot no. PCA1521B, AllCells) for these experiments. The fresh CD133+ cells were cultured with serum-free medium (StemSpan, Stem Cell TechnologiesFull Paper
2012Kadam, SK;Farmen, M;Brandt, JT;Quantitative measurement of cell-free plasma DNA and applications for detecting tumor genetic variation and promoter methylation in a clinical settingThe Journal of molecular diagnostics : JMD22579630Commercially available pooled human plasma (AllCells, Emeryville, CA) was used to determine matrix effect and for DNA spike-in experiments. All blood samples from healthy controls SaveFull Paper
2012Magga, J;Savchenko, E;Malm, T;Rolova, T;Pollari, E;Valonen, P;Lehtonen, Š;Jantunen, E;Aarnio, J;Lehenkari, P;Koistinaho, M;Muona, A;Koistinaho, J;Production of monocytic cells from bone marrow stem cells: therapeutic usage in Alzheimer's diseaseJournal of cellular and molecular medicine21777378Flt-3, 20 ng/ml IL-6, 20 ng/ml IL-3, in humidified atmosphere at 37°C in 5% CO2. Cells were cultivated and differentiated as described earlier. Human PB GCSF-mobilized CD34 cells were obtained from AllCells and cultivated similarly to BM-originated cFull Paper
2012Xia, Y;Wübbeler, JH;Qi, Q;Steinbüchel, A;Employing a recombinant strain of Advenella mimigardefordensis for biotechnical production of Homopolythioesters from 3,3'-dithiodipropionic acidApplied and environmental microbiology22344658AftertheirODhadreached500Klettunits,allcells were transferred into the same MS medium containing 7 g/liter glycerol plus 9 g/liter DTDP as a precursor. (A) Increase of OD (Klett unit) SaveFull Paper
2012Pei, L;Choi, JH;Liu, J;Lee, EJ;McCarthy, B;Wilson, JM;Speir, E;Awan, F;Tae, H;Arthur, G;Schnabel, JL;Taylor, KH;Wang, X;Xu, D;Ding, HF;Munn, DH;Caldwell, C;Shi, H;Genome-wide DNA methylation analysis reveals novel epigenetic changes in chronic lymphocytic leukemiaEpigenetics22534504CD19+/CD27+ memory B-cell, and CD19+/CD27- naïve B-cell samples used in the RRBS analysis were purchased from ALLCELLs Inc. Other normal CD19+ B-cell samples were purified from leuko-enriched blood samples purchased from a local blood bank. MononucleFull Paper
2012Behbehani, GK;Bendall, SC;Clutter, MR;Fantl, WJ;Nolan, GP;Single-cell mass cytometry adapted to measurements of the cell cycleCytometry. Part A : the journal of the International Society for Analytical Cytology22693166Mass cytometry is a recently introduced technology that utilizes transition element isotope‐tagged antibodies for protein detection on a single‐cell basis. By circumventing the limitations SaveFull Paper
2012Kanagal-Shamanna, R;Zhao, W;Vadhan-Raj, S;Nguyen, MH;Fernandez, MH;Medeiros, LJ;Bueso-Ramos, CE;Over-expression of CYP2E1 mRNA and protein: implications of xenobiotic induced damage in patients with de novo acute myeloid leukemia with inv(16)(p13.1q22); CBFβ-MYH11International journal of environmental research and public health23066397Island, NY, USA). cDNA synthesis was performed using the QuantiTect RT Kit (Qiagen, Valencia, CA, USA) according to manufacturer’s instructions [14]. The commercially produced normal BM CD34 cDNA (AllCells, LLC, Emeryville, CA, USA) was also used inFull Paper
2012Puri, N;Sodhi, K;Haarstad, M;Kim, DH;Bohinc, S;Foglio, E;Favero, G;Abraham, NG;Heme induced oxidative stress attenuates sirtuin1 and enhances adipogenesis in mesenchymal stem cells and mouse pre-adipocytesJournal of cellular biochemistry22234917For human MSCs, frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential SaveFull Paper
2012Spencer, R;Magnetic resonance in tissue engineeringNMR in biomedicine22134896Mesenchymal stem cells isolated from fresh adult human bone marrow were obtained commercially by AllCells (AllCells LLC, Berkeley, CA, USA). Nucleated cells were incubated in a SaveFull Paper
2012Guo, LW;Wu, Q;Green, B;Nolen, G;Shi, L;Losurdo, J;Deng, H;Bauer, S;Fang, JL;Ning, B;Cytotoxicity and inhibitory effects of low-concentration triclosan on adipogenic differentiation of human mesenchymal stem cellsToxicology and applied pharmacology22726953Human adult mesenchymal stem cells (hMSCs) were originally provided by AllCells (Emeryville, CA). hMSCs were grown in alpha Minimum Essential Medium (αMEM), supplemented SaveFull Paper
2012Xu, D;Kim, Y;Postelnek, J;Vu, MD;Hu, DQ;Liao, C;Bradshaw, M;Hsu, J;Zhang, J;Pashine, A;Srinivasan, D;Woods, J;Levin, A;O'Mahony, A;Owens, TD;Lou, Y;Hill, RJ;Narula, S;DeMartino, J;Fine, JS;RN486, a selective Bruton's tyrosine kinase inhibitor, abrogates immune hypersensitivity responses and arthritis in rodentsThe Journal of pharmacology and experimental therapeutics22228807One million human cord blood-derived CD34+ hematopoietic stem cells (AllCells, LLC, Emeryville, CA) were cultured in a serum-free StemPro-34 complete medium (Invitrogen) with supplements, stem cell factor (100 ng/ml), and IL-6 (50 ng/ml). IL-3 (10 ngFull Paper
2012Pirlo, RK;Wu, P;Liu, J;Ringeisen, B;PLGA/hydrogel biopapers as a stackable substrate for printing HUVEC networks via BioLPBiotechnology and bioengineering21830203Two major challenges in tissue engineering are mimicking the native cell-cell arrangements of tissues and maintaining viability of three‐dimension (3D) tissues thicker than 300 µm. SaveFull Paper
2012Zou, C;Chou, BK;Dowey, SN;Tsang, K;Huang, X;Liu, CF;Smith, C;Yen, J;Mali, P;Zhang, YA;Cheng, L;Ye, Z;Efficient derivation and genetic modifications of human pluripotent stem cells on engineered human feeder cell linesStem cells and development22225458Bone marrow-derived MSCs were established from CD34-depleted mononuclear cells (MNCs) (AllCells) as previously described [16], from adult donors (such as a male donor #BM2426). Primary MSCs and immortalized MSCs were cultured in Dulbecco's modified EFull Paper
2012Aslanidi, GV;Rivers, AE;Ortiz, L;Govindasamy, L;Ling, C;Jayandharan, GR;Zolotukhin, S;Agbandje-McKenna, M;Srivastava, A;High-efficiency transduction of human monocyte-derived dendritic cells by capsid-modified recombinant AAV2 vectorsVaccine22497875DMEM (Invitrogen) supplemented with 10% FBS (Sigma) and antibiotics (Lonza). Leukapheresis-derived peripheral blood mononuclear cells (PBMCs) (AllCells) were purified on Ficoll-Paque (GEHeathCare), resuspended in serum-free AIM-V medium (Lonza), andFull Paper
2012Koestler, DC;Marsit, CJ;Christensen, BC;Accomando, W;Langevin, SM;Houseman, EA;Nelson, HH;Karagas, MR;Wiencke, JK;Kelsey, KT;Peripheral blood immune cell methylation profiles are associated with nonhematopoietic cancersCancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology22714737Sorted, normal, human, peripheral blood leukocyte subtypes were purchased from AllCells. Leukocytes were isolated from different, anonymous, nondiseased individuals' whole blood ( SaveFull Paper
2012Hong, L;Wei, N;Joshi, V;Yu, Y;Kim, N;Krishnamachari, Y;Zhang, Q;Salem, AK;Effects of glucocorticoid receptor small interfering RNA delivered using poly lactic-co-glycolic acid microparticles on proliferation and differentiation capabilities of human mesenchymal stromal cellsTissue engineering. Part A21988716All other chemicals and solvents used, including dichloromethane (DCM) and acetonitrile, were of high-performance liquid chromatography analytical grade. Fresh human bone marrow was purchased from Allcells (Emeryville, CA). Invitrogen (Carlsbad, CA)Full Paper
2012Noto, PB;Bukhtiyarov, Y;Shi, M;McKeever, BM;McGeehan, GM;Lala, DS;Regulation of sphingomyelin phosphodiesterase acid-like 3A gene (SMPDL3A) by liver X receptorsMolecular pharmacology22810003Whole blood from human donors was purchased from AllCells, LLC (Emeryville, CA). Human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood collected SaveFull Paper
2012Abdolhosseini, M;Nandula, SR;Song, J;Hirt, H;Gorr, SU;Lysine substitutions convert a bacterial-agglutinating peptide into a bactericidal peptide that retains anti-lipopolysaccharide activity and low hemolytic activityPeptides22484285GL13NH2 is a bacteria-agglutinating peptide derived from the sequence of the salivary protein parotid secretory protein (PSP, BPIFA2, SPLUNC2, C20orf70). The peptide agglutinates SaveFull Paper
2012Berezhnyy, IV;Berezhna, SY;Fast multi-spectral imaging technique for detection of circulating endothelial cells in human blood samplesJournal of biomedical optics23224165Blood samples from healthy donors were purchased from AllCells, LLC., Emeryville, CA. The blood was collected via venipuncture in EDTA tubes, stored and delivered at 4°C. Before processing, a white blood cell count was performed using Scepter™ 2.0 ceFull Paper
2012Shah, SN;Kerr, C;Cope, L;Zambidis, E;Liu, C;Hillion, J;Belton, A;Huso, DL;Resar, LM;HMGA1 reprograms somatic cells into pluripotent stem cells by inducing stem cell transcriptional networksPloS one23166588tocol# MO11M279). All mice were housed in a sterile environment where they had free access to food and water as outlined in our institutional guidelines. Cell Culture MSC1640 mesenchymal stem cells (AllCells, LLC) were maintained in DMEM (low glucosFull Paper
2012Ringpis, GE;Shimizu, S;Arokium, H;Camba-Colón, J;Carroll, MV;Cortado, R;Xie, Y;Kim, PY;Sahakyan, A;Lowe, EL;Narukawa, M;Kandarian, FN;Burke, BP;Symonds, GP;An, DS;Chen, IS;Kamata, M;Engineering HIV-1-resistant T-cells from short-hairpin RNA-expressing hematopoietic stem/progenitor cells in humanized BLT micePloS one23300932for 2 hours in the presence of 8 μg/ml of polybrene. Transductions of PBMCs were performed as previously described [23] with indicated cell numbers and MOIs. mPB-CD34+ cells (9.8×104), provided by AllCells (Emeryville, CA), were pre-stimulated with SFull Paper
2012Zhu, J;Siclari, VA;Liu, F;Spatz, JM;Chandra, A;Divieti Pajevic, P;Qin, L;Amphiregulin-EGFR signaling mediates the migration of bone marrow mesenchymal progenitors toward PTH-stimulated osteoblasts and osteocytesPloS one23300521Human bone marrow aspirates were purchased from Allcells and purified through Ficoll gradient (GE Healthcare) to obtain bone marrow mononuclear cells. These cells were seeded SaveFull Paper
2012Wen, H;Li, Y;Malek, SN;Kim, YC;Xu, J;Chen, P;Xiao, F;Huang, X;Zhou, X;Xuan, Z;Mankala, S;Hou, G;Rowley, JD;Zhang, MQ;Wang, SM;New fusion transcripts identified in normal karyotype acute myeloid leukemiaPloS one23251452ry samples with abnormal karyotype AML and eight AML primary samples without karyotype information [Figure 1], of which 38 were from University of Michigan Comprehensive Cancer Center and 7 were from Allcells Inc (AllCells, CA). The use of samples frFull Paper
2012Kobayashi, DT;Decker, D;Zaworski, P;Klott, K;McGonigal, J;Ghazal, N;Sly, L;Chung, B;Vanderlugt, J;Chen, KS;Evaluation of peripheral blood mononuclear cell processing and analysis for Survival Motor Neuron proteinPloS one23226377A: SMN signal was evaluated in lysates created from a single sample purchased from AllCells. PBMCs were processed in ER4 at densities of 10 6 , 10 7 and 5×10 7 cells/mL and the SaveFull Paper
2012Zhang, LJ;Tao, BB;Wang, MJ;Jin, HM;Zhu, YC;PI3K p110α isoform-dependent Rho GTPase Rac1 activation mediates H2S-promoted endothelial cell migration via actin cytoskeleton reorganizationPloS one22970259uman recombinant Rac1 and G-LISA Rac1 activation assay kits were obtained from Cytoskeleton (Denver, CO, USA). Cell culture-Primary human umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Emeryville, CA, USA). Each batch of HUVEFull Paper
2012Venet, S;Ravn, U;Buatois, V;Gueneau, F;Calloud, S;Kosco-Vilbois, M;Fischer, N;Transferring the characteristics of naturally occurring and biased antibody repertoires to human antibody libraries by trapping CDRH3 sequencesPloS one22937053Human CDRH3 from three donors were amplified by nested PCR from commercially available peripheral blood purified cells cDNA (AllCells and BioChain). The first PCR allowed the SaveFull Paper
2012Park, TS;Huo, JS;Peters, A;Talbot, CC;Verma, K;Zimmerlin, L;Kaplan, IM;Zambidis, ET;Growth factor-activated stem cell circuits and stromal signals cooperatively accelerate non-integrated iPSC reprogramming of human myeloid progenitorsPloS one22905176For these experiments, highly purified (>96%) CD34 + CD45 + CB cells were received within 24 hours of neonatal harvest from AllCells (catalog number: CB005). On the same day (day SaveFull Paper
2012Milanesi, A;Lee, JW;Li, Z;Da Sacco, S;Villani, V;Cervantes, V;Perin, L;Yu, JS;β-Cell regeneration mediated by human bone marrow mesenchymal stem cellsPloS one22879915BMSCs from a normal adult donor were purchased from a commercial source AllCells, LLC (5858 Horton Street, Suite 360, Emeryville, California, 94608 USA; catalog number ABM005) SaveFull Paper
2012Boomsma, RA;Geenen, DL;Mesenchymal stem cells secrete multiple cytokines that promote angiogenesis and have contrasting effects on chemotaxis and apoptosisPloS one22558198TCTCCTC GTCTGCCTCTTTTGGTCAGG Angiogenesis Assay The effect of paracrine factors secreted by MSC into CM on angiogenesis was studied using canine jugular vein vascular endothelial cells (CVEC; AllCells) and the Fibrin Gel In Vitro AngiogenesisFull Paper
2012Basile, LA;Ellefson, D;Gluzman-Poltorak, Z;Junes-Gill, K;Mar, V;Mendonca, S;Miller, JD;Tom, J;Trinh, A;Gallaher, TK;HemaMax™, a recombinant human interleukin-12, is a potent mitigator of acute radiation injury in mice and non-human primatesPloS one22383962clear cells (PBMC) in vitro and the PK and PD characteristics of HemaMax in vivo. Isolation of CD14- PBMC and Quantification of IFN-γ Secretion Human PBMC collected by apheresis were purchased from AllCells (Emeryville, CA, USA). Mouse and rhesus moFull Paper
2012Gao, N;Cheng, S;Budhraja, A;Liu, EH;Chen, J;Chen, D;Yang, Z;Luo, J;Shi, X;Zhang, Z;3,3'-Diindolylmethane exhibits antileukemic activity in vitro and in vivo through a Akt-dependent processPloS one22363731lls were washed and assayed for total number and viability using trypan blue exclusion. Cells were suspended at 8×105/mL for treatment. Fresh normal bone marrow mononuclear cells were purchased from Allcells (Emeryvill, CA). After washing and enumeraFull Paper
2012Rellick, SL;O'Leary, H;Piktel, D;Walton, C;Fortney, JE;Akers, SM;Martin, KH;Denvir, J;Boskovic, G;Primerano, DA;Vos, J;Bailey, N;Gencheva, M;Gibson, LF;Bone marrow osteoblast damage by chemotherapeutic agentsPloS one22363485Mediatech), 0.05 µM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), non-essential amino acids and B-FGF solution. H9 cells were moved to HOB feeder layers where indicated. CD34+ bone marrow cells (ALLCELLS, Emeryville, CA) were grown in RPMI-1640 sFull Paper
2012Houseman, EA;Accomando, WP;Koestler, DC;Christensen, BC;Marsit, CJ;Nelson, HH;Wiencke, JK;Kelsey, KT;DNA methylation arrays as surrogate measures of cell mixture distributionBMC bioinformatics22568884ples, de-identified specimens that were not subject to human subjects review by an institutional review board (IRB). The sorted, normal, human, peripheral blood leukocyte subtypes were purchased from AllCellsⓇ, LLC (Emeryville, CA) and were isolatedFull Paper
2012Christopherson, KW;Frank, RR;Jagan, S;Paganessi, LA;Gregory, SA;Fung, HC;CD26 protease inhibition improves functional response of unfractionated cord blood, bone marrow, and mobilized peripheral blood cells to CXCL12/SDF-1Experimental hematology22846168Medical Center (RUMC) with institutional review board (IRB) approval. Human G-CSF mobilized peripheral blood (mobPB) was purchased from AllCells, LLC (Emeryville, CA, USA). Mononuclear cells (MNC) were isolated by density gradient centrifugation overFull Paper
2012Iancu-Rubin, C;Gajzer, D;Mosoyan, G;Feller, F;Mascarenhas, J;Hoffman, R;Panobinostat (LBH589)-induced acetylation of tubulin impairs megakaryocyte maturation and platelet formationExperimental hematology22381681Human MK were generated in liquid cultures from bone marrow (BM)-derived CD34+ cells (AllCells, Inc. Emeryville, CA). Cells were suspended in IMDM medium (Invitrogen, Grand Island, NY) supplemented with 1% penicillin/streptomycin, 1% L-Glutamine (InvFull Paper
2012Fujioka, Y;Tanaka, N;Nakanishi, K;Kamei, N;Nakamae, T;Izumi, B;Ohta, R;Ochi, M;Magnetic field-based delivery of human CD133⁺ cells promotes functional recovery after rat spinal cord injurySpine22246536Phosphate-buffered saline (PBS) solution (50 μL) containing 1 × 10 5 of human peripheral blood-derived CD133 + cells (AllCells, Emeryville, CA) was injected into the subarachnoid SaveFull Paper
2012Huovinen, P;Petersen, H;Particlization in hybrid modelsThe European Physical Journal AThe first one that we call "allcells" sampling, contains a loop over all hypersurface elements The straightforward "allcells" sampling does not do it, and therefore we have developed the SaveFull Paper
2012Kusao, I;Shiramizu, B;Liang, CY;Grove, J;Agsalda, M;Troelstrup, D;Velasco, VN;Marshall, A;Whitenack, N;Shikuma, C;Valcour, V;Cognitive performance related to HIV-1-infected monocytesThe Journal of neuropsychiatry and clinical neurosciences22450616assay for IL6, IL8, IL10, TNFα, sFAS, sTNFRI, sTNFRII, and MCP1. For the in-vitro experiments, monocytes were separated from commercially-available PBMC (AllCells; Emeryville, CA) using the EasySep Human CD14 Positive Selection Kit (Stem Cell TechnolFull Paper
2012Yu, Y;Wei, N;Stanford, C;Schmidt, T;Hong, L;In vitro effects of RU486 on proliferation and differentiation capabilities of human bone marrow mesenchymal stromal cellsSteroids22093480Fresh human bone marrows, donated by healthy donors from 21 to 47 years of age, were purchased from Allcells (Emeryville, CA). Mononuclear cells were isolated using density SaveFull Paper
2012Tatsumi, K;Taatjes, DJ;Wadsworth, MP;Bouchard, BA;Bovill, EG;Cell adhesion molecule 1 (CADM1) is ubiquitously present in the endothelium and smooth muscle cells of the human macro- and micro-vasculatureHistochemistry and cell biology22940845Immunostained cells were visualized with an Olympus BX51 fluorescence microscope (Olympus America, Center Valley, PA). For each experiment (n = 3) human umbilical vein ECs (AllCells, Emeryville CA), cultured as per the manufacturer’s directions, wereFull Paper
2012He, M;Chen, P;Arnovitz, S;Li, Y;Huang, H;Neilly, MB;Wei, M;Rowley, JD;Chen, J;Li, Z;Two isoforms of HOXA9 function differently but work synergistically in human MLL-rearranged leukemiaBlood cells, molecules & diseases22633751The mononuclear cell (MNC) samples were isolated from normal BM cells purchased from AllCells, LLC. MNC cells were isolated using NycoPrep 1.077A (Axis-Shield, Oslo, Norway) Save;in Dulbecco modified Eagle's medium (DMEM) supplemented with 10% FBSFull Paper
2012Kobayashi, M;Kawase, T;Horimizu, M;Okuda, K;Wolff, LF;Yoshie, H;A proposed protocol for the standardized preparation of PRF membranes for clinical useBiologicals : journal of the International Association of Biological Standardization22841724Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells, LLC (Emeryville, CA) and maintained in an endothelial cell medium that was fully supplemented with growth factors (HuMedia-EB2; KURABO, Osaka, Japan). For the scratch assay,Full Paper
2012Aoki, T;Miyazaki, K;Katayama, T;Watanabe, M;Horie, R;Danbara, M;Higashihara, M;Surface CD3 expression proceeds through both myosin regulatory light chain 9 (MYL9)-dependent and MYL9-independent pathways in Jurkat cellsJournal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi23538510K562 cells were transfected using the 96-well Nucleofector Kit SF (Lonza) per manufacturer's recommendations. PBMCs (AllCells) were received on ice, centrifuged at 1,000g for 5 min at room temperature (22 °C) and changed to pre-warmed RPMI medium supFull Paper
2012Wakao, S;Kitada, M;Kuroda, Y;Dezawa, M;Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damagesMethods in molecular biology (Clifton, N.J.)22167642candidate cell source for isolation of Muse cells. We recommend to use adult human dermal fibroblasts (CC-2511, Lonza, Basel, Switzerland; 2320, ScienCell, Carlsbad, CA) and adult human bone marrow stromal cells (PT-2501, Lonza; ABM001, AllCells, EmeFull Paper
2012Mendelson, A;Chondrogenesis of Stem/Progenitor Cells by Chemotaxis Using Novel Cell Homing SystemsThesisHuman bone marrow MSCs were isolated from fresh adult donor samples (AllCells, Emeryville, CA) using our prior methods [94, 96, 115]. Briefly, the bone marrow sample was SaveFull Paper
2012Balzano, G;Liesch, B;The role of chroma and scalestep in the recognition of musical intervals in and out of context.Psychomusicology: A Journal of Research in Music CognitionThe octave presented somewhat of a problem in these analyses; latencies (and errors) tended to be drastically lower in allcells ofthe matrix involving the octave, and because of the SaveFull Paper
2012Gratien, J;Implementing Lowest-Order Methods for Diffusive Problems with a DSELModelling and Simulation in Fluid Dynamics in Porous MediaThe integration algorithm consists in iterating on the cell elements of the allCells(Th) and evaluating the bilinear expression on each cell. This bilinear expression is composed of: SaveFull Paper
2012YAN, LI;Improvements on Humanized NOD/SCID lL-2rg-/-MiceThesisPurified human CD133+ cord blood cells were purchased from AllCells (California). Alternatively, umbilical cord blood was obtained from the Singapore Cord Blood Bank. Red blood SaveFull Paper
2012Nikdel, A;Jameii, SM;Faez, K;A novel area coverage protocol based on particle swarm optimization algorithm for wireless sensor networksGlobal Journal on Technologywill be provided if the sum of cells which are covered (ie Sumcells set), are equal to the sum of cells which will be covered if all nodes have maximum sensing radius (ie Allcells set). As SaveFull Paper
2012Huff, RR;Induction of chondrogenesis from MSCs by BMP7 and TGFB1 in low oxygen tension: expression of superficial zone proteinThesisThe IRC received the sample as whole bone marrow, purchased commercially (AllCells Inc.). To isolate the MSCs, nucleated cells were recovered from a 25mL sample using a Ficoll- SaveFull Paper
2012Sunderic, K;Effects of Mild Heating on the Osteogenic Differentiation of Human Mesenchymal Stem Cells during InflammationThesisHuman bone marrow explanted from the iliac crest of a 24-year old donor was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the SaveFull Paper
2012Sangokoya, CO;MicroRNA Function in Cellular Stress ResponseThesisHbAA CD34+ cells (ALLCells) and HbSS CD34+ cells were purified from blood collected from apheresis with donor consent according to Duke IRB protocol. Mononuclear cells were separated by Ficoll-Hypaque density gradient, and CD34+ cells were enriched bFull Paper
2012McNamara, LA;HIV-1 Infection of Hematopoietic Progenitor Cells.ThesisWe obtained pre-existing umbilical cord blood lacking subject identifiers after scheduled cesarean section procedures. We obtained fresh whole bone marrow aspirates from a commercial source (AllCells Ltd.). We prepared BMMCs and UCB mononuclear cellsFull Paper
2012Lowe, RM;Investigation into the role of novel interferon lambdas in the treatment of viral disease, primarily hepatitis CThesis+L-glutamine and 100ng/mL Macrophage colony-stimulating factor (M-CSF) and incubated for four days to differentiate into macrophages. Mast cells were derived from cord blood mononuclear cells (AllCells), cells were cultured in Iscove’s modified DulbeFull Paper
2012David, G;Le locus 1q32: susceptibilité aux maladies inflammatoires de l'intestin et rôles biologiques de C1orf106 et KIF21BThesispréparations d’ARN ont été conservées à -80°C. Les ARN des cellules primaires CD4+, CD8+, CD14+ et CD19+ proviennent de la compagnie Allcells (Californie, États-Unis)Full Paper
2012Lin, X;Novel Factors in Myeloproliferative Neoplasms-The Role of NFIB and miRNA Deregulation in Hematopoietic Cell Proliferation and DifferentiationThesisJAK2V617F mutation was detected in 7 out of 9 specimens by reverse transcription/PCR (AmpliTaq Gold) and directly sequencing JAK2 sequences of 1849 nucleotides amplified. Normal bone marrow CD34+ cells were purchased from AllCells. To isolate CD34+ cFull Paper
2012Budhraja, A;Molecular mechanism of apigenin induced apoptosis in leukemiaThesisNormalperipheral blood mononuclear cells were obtained from AllCells (Emeryville, CA, USA)and were maintained in RPMI 1640 supplemented with 10% FBS. Dr. Ruth Craig( SaveFull Paper
2012Severson, EBS;Developing a Novel In-Vitro Neuroinflammation Model for Huntington's DiseaseThesisHuman BM aspirates were purchased from Lonza and AllCells. Density gradient centrifugation (Ficoll-Hypaque) was used to isolate mononuclear BM-MSC from three donors (AC1, SaveFull Paper
2012Mahmoud, AEM;Investigating the biochemical activity of some flavonoids, coumarins and modified gold-nanoparticles as anti-breast-cancer agentsThesisAllcells were incubated for 2 days at 37 C under 5 % CO2. When the cells reached 50%confluent, the old media was removed and the adherent cells were first washed by IXversene. SaveFull Paper
2012Suh, SS;A study of microRNAs associated with multiple myeloma pathogenesis and microRNAs/TP53 feedback circuit in human cancers, multiple myeloma and glioblastoma multiformeThesiswith monoclonal mouse anti-human CD138 antibodies using the AutoMACs automated separation system (Miltenyi-Biotec Three of the six healthy PCs were obtained from ALLCELLS, SaveFull Paper
2012Berk, E;Dendritic cells regulate the induction of effector and memory CD8+ t cellsThesisSW480 and SW620 (ATCC, Manassas, VA) were grown in IMDM+10% FBS, while HUVECs (AllCells, Emeryville, CA) were cultured in HUVEC complete media (Basal media supplemented with HUVEC stimulatory supplement; AllCells). All were washed, reseeded at 20,000Full Paper
2012Peters, HLC;Characteristics of amyloid precursor-like protein 2 (APLP2) in pancreatic cancer and Ewing's sarcomaThesisAllcells and cellular debris were pelleted by centrifugation at 2,000 xg at 4C for 5 min. Thesupernatant was discarded and pellets containing 5 x 106 cells were resuspended in 100/jL SaveFull Paper
2012Chung, TY;Experimental Beam Studies of Plasma-generated Species Interaction with Polymeric Materials and BiomoleculesThesisThe basic protocol is shown in Figure 3.6. Heparinized fresh human whole blood was acquired from AllCells (Emeryville, CA, USA) and used within 6 h of collection. Samples were prepared by spotting serial diluted lipid A solution on UV/ozone-cleaned sFull Paper
2012Hung, KC;Developing peptide and antibody-mimetic ligands for the cell surface receptor beta2AR and DC-SIGNThesisSallusto and Lanzavecchia, 1994), except that hPBMCs were from HLA-A2+ patients (AllCells, LLC.). iDCs were treated with M3H4-18-HA, stained with anti-HA antibody, and analyzed by either flow cytometry or microscopy same as 293T cells, except that thFull Paper
2012Simonds, EF;Single-Cell Deep Profiling of Immune Signaling and Drug Responses in Normal and Malignant Human HematopoiesisThesisobtained from a tissue bank at Princess Margaret Hospital (Toronto, ON), courtesy of Dr. Mark D. Minden. Cryopreserved healthy BMMCs were purchased from AllCells, Inc. (Emeryville, CA). All human samples were obtained with informed consent in compliaFull Paper
2012Pardee, TS;Gomes, E;Jennings-Gee, J;Caudell, D;Gmeiner, WH;Unique dual targeting of thymidylate synthase and topoisomerase1 by FdUMP[10] results in high efficacy against AML and low toxicityBlood22362039Human cell lines, primary patient samples, or normal human HSCs were thawed and incubated in Stem SpanSFEM media supplemented with Stem Span CC100 cytokine cocktail (StemCell Technologies) for 24 hours with the indicated drug. Cells were then placedFull Paper
2012Zeiger, AS;Loe, FC;Li, R;Raghunath, M;Van Vliet, KJ;Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behaviorPloS one22649562ganization correlate with an increase in proliferation and a decrease in motility of MSCs. Materials and Methods Cell Culture Human bone-marrow derived mesenchymal stromal or “stem” cells (MSCs; ReachBio Seattle, WA) were expanded until passage 4-6,Full Paper
2012Jolibois-Quinot, R;Quantitative correlations among human mesenchymal stem cell mechanical properties and biological functionThesisAdult mesenchymal stem cells (referred later as aMSCs) were purchased from commercial sources: ReachBio human bone marrow mesenchymal stem cells - lot number 0090408 (referred later as RB1 - ReachBio LLC, Seattle, WA), and Poietics" human mesenchymalFull Paper
2012Maloney, JM;Chemomechanics of attached and suspended cellsThesisI have also been lucky to work with fine coauthors that dependably contributed honest and top-notch data and ideas: Christopher Bruce and Emily Walton (MIT), Franziska Lautenschläger and Jochen Guck (University of Cambridge), Dessy Nikova (MIT), EmerFull Paper
2011Xu, B;Roos, JL;Dexheimer, P;Boone, B;Plummer, B;Levy, S;Gogos, JA;Karayiorgou, M;Exome sequencing supports a de novo mutational paradigm for schizophreniaNat. Genet.21822266BX, JAG and MK designed the study, interpreted the data and prepared the manuscript; BX developed the analysis pipeline and had the primary role in analysis and validation of sequence data; JLR collected the samples and was the primary clinician on tFull Paper
2011Wood, AM;Van Bortle, K;Ramos, E;Takenaka, N;Rohrbaugh, M;Jones, BC;Jones, KC;Corces, VG;Regulation of chromatin organization and inducible gene expression by a Drosophila insulatorMol. Cell21981916Chromatin immunoprecipitation was performed as described (Bushey et al., 2009). To generate sequencing libraries ChIP DNA was prepared for adaptor ligation by end repair (‘End-It DNA End Repair Kit’ - Epicentre Cat# ER0720) and addition of ‘A’ base tFull Paper
2011Jänne, PA;Boss, DS;Camidge, DR;Britten, CD;Engelman, JA;Garon, EB;Guo, F;Wong, S;Liang, J;Letrent, S;Millham, R;Taylor, I;Eckhardt, SG;Schellens, JH;Phase I dose-escalation study of the pan-HER inhibitor, PF299804, in patients with advanced malignant solid tumorsClin. Cancer Res.21220471Immunohistochemistry analysis was performed by QualTek (Newtown, PA) and was automated throughout using a TechMate 500 or TechMate 1000 (BioTek Solutions/Ventana Medical Systems). Antibodies used were: pERK 1/2 clone 20G11 (Cell Signaling Cat # 4376)Full Paper
2011Ahmad, I;Morton, JP;Singh, LB;Radulescu, SM;β-Catenin activation synergizes with PTEN loss to cause bladder cancer formationOncogeneinvasive bladder carcinoma. We next looked at human bladder UCC using a tissue microarray of 80 cases, 60 UCC (transitional cell carcinomas) and 20 benign controls (Folio Biosciences, Columbus, OH, USA). Using the histoscoreFull Paper
2011Teichert, AE;Elalieh, H;Elias, PM;Welsh, J;Bikle, DD;Overexpression of hedgehog signaling is associated with epidermal tumor formation in vitamin D receptor-null miceJ. Invest. Dermatol.21814234ffinity-purified, biotinylated goat anti-rabbit IgG, followed by ABC-peroxidase reagent, both purchased from Vector (Burlingame, CA). Peroxidase activity was detected with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followedFull Paper
2011Kelly, RK;Olson, DL;Sun, Y;Wen, D;Wortham, KA;Antognetti, G;Cheung, AE;Orozco, OE;Yang, L;Bailly, V;Sanicola, M;An antibody-cytotoxic conjugate, BIIB015, is a new targeted therapy for Cripto positive tumoursEur. J. Cancer214589842.3. Immunohistochemistry. Human breast, colon, lung and ovarian formalin-fixed paraffin-embedded tumour tissue arrays (Imgenex, San Diego, CA, Folio Biosciences, Columbus, OH and Zymed, San Francisco, CA) were stained as described previouslyFull Paper
2011Ahmad, I;Singh, LB;Foth, M;Morris, CA;Taketo, MM;Wu, XR;Leung, HY;Sansom, OJ;Iwata, T;K-Ras and β-catenin mutations cooperate with Fgfr3 mutations in mice to promote tumorigenesis in the skin and lung, but not in the bladderDis Model Mech21504907Finally, we have examined the ERK-MAPK signaling pathway in human UCC by using a tissue microarray (TMA) that contains 60 UCC and 20 benign controls (Folio Biosciences, OH). Using the Histoscore technique, we have quantified the staining intensitiesFull Paper
2011Rokkam, D;Lafemina, MJ;Lee, JW;Matthay, MA;Frank, JA;Claudin-4 levels are associated with intact alveolar fluid clearance in human lungsAm. J. Pathol.21741940controls. Additional normal lung tissue specimens (n = 3) were also obtained (US Biomax, Rockville, MD; Folio Biosciences, Columbus, OH) and immunostained for claudin-4, as previously described. Histology Scoring. TheFull Paper
2011Kent, BN;Salichos, L;Gibbons, JG;Rokas, A;Newton, IL;Clark, ME;Bordenstein, SR;Complete bacteriophage transfer in a bacterial endosymbiont (Wolbachia) determined by targeted genome captureGenome Biol Evol21292630Genomic DNA was extracted from ∼200 abdomens of the B-Wolbachia infected N. vitripennis 4.9 using the QIAGEN DNeasy Kit insect tissue protocol. DNA was treated with 100 mg/ml RNaseA and incubated at room temperature for 2 min. The resulting DNA was qFull Paper
2011Jiang, Y;Lu, J;Peatman, E;Kucuktas, H;Liu, S;Wang, S;Sun, F;Liu, Z;A pilot study for channel catfish whole genome sequencing and de novo assemblyBMC Genomics22192763The culture supernatant was decanted and the block was inverted and tapped gently on paper towels to remove remaining liquid. BAC DNA was isolated using the Perfectprep™ BAC 96 kit (Eppendorf North America, Westbury, NY) according to the manufacturerFull Paper
2011Pitts, RJ;Rinker, DC;Jones, PL;Rokas, A;Zwiebel, LJ;Transcriptome profiling of chemosensory appendages in the malaria vector Anopheles gambiae reveals tissue- and sex-specific signatures of odor codingBMC Genomics21619637We thank the following individuals: Zhen Li for mosquito rearing; Travis Clark, Chelsea Baker and the Vanderbilt Genomics Shared Resource for sample preparation and Illumina sequencing; Shawn Levy and the Genomic Services Lab at HudsonAlpha InstituteFull Paper
2011Liu, S;Zhou, Z;Lu, J;Sun, F;Wang, S;Liu, H;Jiang, Y;Kucuktas, H;Kaltenboeck, L;Peatman, E;Liu, Z;Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP arrayBMC Genomics21255432;... sing the RNeasy plus Mini Kit (Qiagen, Valencia, CA, USA) with DNase I (Invitrogen, USA) treatment following the manufacturer's protocol. Illumina sequencing Sequencing was conducted commercially in HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2011Weiss, L;Bernstein, S;Jones, R;Amunugama, R;Krizman, D;Jebailey, L;Almogi-Hazan, O;Hazan, O;Yekhtin, Z;Yachtin, J;Shiner, R;Reibstein, I;Triche, E;Slavin, S;Or, R;Barnea, ER;Preimplantation factor (PIF) analog prevents type I diabetes mellitus (TIDM) development by preserving pancreatic function in NOD miceEndocrine21424847Department of Bone Marrow Transplantation and Cancer Immunotherapy, Hadassah University Hospital Ein Kerem, Hebrew University, Jerusalem, Israel Lola Weiss, Osnat Hazan, Janna Yachtin, Reut Shiner, Israel Reibstein & Reuven Or QualTek Molecular LaborFull Paper
2011Pawar, H;Kashyap, MK;Sahasrabuddhe, NA;Renuse, S;Harsha, HC;Kumar, P;Sharma, J;Kandasamy, K;Marimuthu, A;Nair, B;Rajagopalan, S;Maharudraiah, J;Premalatha, CS;Kumar, KV;Vijayakumar, M;Chaerkady, R;Prasad, TS;Kumar, RV;Kumar, RV;Pandey, A;Quantitative tissue proteomics of esophageal squamous cell carcinoma for novel biomarker discoveryCancer Biol. Ther.21743296The ESCCs varied from tumor grade I to grade III from patients in the age group of 39-79 y. A second group of tissue microarrays obtained from FolioBio (Columbus, OH) consisted of 40 ESCC tissues with matched adjacent normal esophageal squamous epithFull Paper
2011Bunting, RA;Duffy, KE;Lamb, RJ;San Mateo, LR;Smalley, K;Raymond, H;Liu, X;Petley, T;Fisher, J;Beck, H;Flavell, RA;Alexopoulou, L;Ward, CK;Novel antagonist antibody to TLR3 blocks poly(I:C)-induced inflammation in vivo and in vitroCell. Immunol.21092943Mouse tissues (pancreas, inguinal and mesenteric lymph node, brain, liver, heart, trachea, colon, kidneys, lungs, and spleen) from three C57BL/6 and three TLR3KO mice were fixed in 10% neutral buffered formalin and processed for immunohistochemistryFull Paper
2011Trejos, AL;Jayaraman, S;Patel, RV;Naish, MD;Schlachta, CM;Force sensing in natural orifice transluminal endoscopic surgerySurg Endosc20559663allowing the flexible shaft to bend freely. The shaft and the wires were covered with heat-shrink thin-wall tubing (0.26 mm wall thickness, model Q-150K 1/8-02-QB481N- 25; Qualtek Electronics). The 2.8-mm instruments with theFull Paper
2011Karkera, J;Steiner, H;Li, W;Skradski, V;Moser, PL;Riethdorf, S;Reddy, M;Puchalski, T;Safer, K;Prabhakar, U;Pantel, K;Qi, M;Culig, Z;The anti-interleukin-6 antibody siltuximab down-regulates genes implicated in tumorigenesis in prostate cancer patients from a phase I studyProstate21321981PSA, IL‐6R, alpha‐methylacyl‐CoA racemase (AMACR), AR, and Chromogranin A were tested in tumor tissue samples at Qualtek Laboratories (Goleta, CA). Steam heat‐induced epitope retrieval (SHIER) method was used and immunohistochemical procedure was perFull Paper
2011Lynch, FC;A method for following patients with retrievable inferior vena cava filters: results and lessons learned from the first 1,100 patientsJ Vasc Interv Radiol21903414Full Paper
2011Shaw, CM;Scorza, LB;Waybill, PN;Singh, H;Lynch, FC;Optional vena cava filter use in the elderly populationJ Vasc Interv Radiol21530308Full Paper
2011Lynch, FC;Removal of a Günther Tulip filter after 3,006 daysJ Vasc Interv Radiol21277802Full Paper
2011Maxwell, SA;Cherry, EM;Bayless, KJ;Akt, 14-3-3ζ, and vimentin mediate a drug-resistant invasive phenotype in diffuse large B-cell lymphomaLeuk. Lymphoma21323512Preparation of DLBCL tissue extracts for Western blotting Normal and DLBCL lymphatic tissues were pro- cured through Folio Biosciences (Foliobio.com). Frozen tissue was ground to a powder with a pestle in a mortar while immersed in liquid nitrogenFull Paper
2011Farris, MH;Duffy, C;Findlay, RH;Olson, JB;Streptomyces scopuliridis sp. nov., a bacteriocin-producing soil streptomyceteInt. J. Syst. Evol. Microbiol.20870885; sequencing facility. Genomic DNA isolated from strain RB72 T using the method of Bollet et al. (1991) was sent to the HudsonAlpha Genomic Services Lab (Huntsville, AL) for Illumina Genome Analyser IIx sequencing. 16S rRNAFull Paper
2011Lee, LF;Axtell, R;Tu, GH;Logronio, K;Dilley, J;Yu, J;Rickert, M;Han, B;Evering, W;Walker, MG;Shi, J;de Jong, BA;Killestein, J;Polman, CH;Steinman, L;Lin, JC;IL-7 promotes T(H)1 development and serum IL-7 predicts clinical response to interferon-β in multiple sclerosisScience translational medicine21795588Human mononuclear cells were prepared from the buffy coats of samples from healthy adult donors (Stanford Blood Center) or from cord blood purchased from AllCells on Ficoll-Paque gradients. CD4+ T cells were isolated by magnetic bead depletion of CD1Full Paper
2011Topp, MS;Kufer, P;Gökbuget, N;Goebeler, M;Klinger, M;Neumann, S;Horst, HA;Raff, T;Viardot, A;Schmid, M;Stelljes, M;Schaich, M;Degenhard, E;Köhne-Volland, R;Brüggemann, M;Ottmann, O;Pfeifer, H;Burmeister, T;Nagorsen, D;Schmidt, M;Lutterbuese, R;Reinhardt, C;Baeuerle, PA;Kneba, M;Einsele, H;Riethmüller, G;Hoelzer, D;Zugmaier, G;Bargou, RC;Targeted therapy with the T-cell-engaging antibody blinatumomab of chemotherapy-refractory minimal residual disease in B-lineage acute lymphoblastic leukemia patients results in high response rate and prolonged leukemia-free survivalJournal of clinical oncology : official journal of the American Society of Clinical Oncology21576633Shown is the analysis of DNA fragmentation in B-lineage ALLcells after 48 hours of culture in serum-free medium alone. In this DNA fragmentation assay, intact chromatin and SaveFull Paper
2011Yoneda, A;Kurokawa, T;A sensitive sandwich ELISA to measure (1→ 3)-β-d-glucan levels in bloodJournal of immunological methods2009). 2.7. Measurement of plasma β-glucan. For the measurements of β-glucan level in human plasma, we used commercially available β-glucan-positive human plasma samples purchased from Discovery Life Sciences, IncFull Paper
2011Mayer, JA;Pham, T;Wong, KL;Scoggin, J;Sales, EV;FISH-based determination of HER2 status in circulating tumor cells isolated with the microfluidic CEE™ platformCancer geneticsPeripheral blood was collected under appropriate third-party institutional review board-approved protocols (ConversantBIO, Huntsville, AL; BioOptions, Fullerton, CA) and delivered to Biocept's CLIA/CAP accredited laboratoryFull Paper
2011Lundgren, JA;Matsushima, K;Lynch, FC;Frankel, H;Cooney, RN;Angiographic embolization of nonvariceal upper gastrointestinal bleeding: predictors of clinical failureJ Trauma21610434Full Paper
2011Balazs, AB;Chen, J;Hong, CM;Rao, DS;Yang, L;Baltimore, D;Antibody-based protection against HIV infection by vectored immunoprophylaxisNature22139420Human peripheral mononuclear blood cells (AllCells) were thawed from −80 C, expanded in RPMI medium supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin mix( SaveFull Paper
2011Sigal, A;Kim, JT;Balazs, AB;Dekel, E;Mayo, A;Milo, R;Baltimore, D;Cell-to-cell spread of HIV permits ongoing replication despite antiretroviral therapyNature21849975Anonymous PBMCs or peripheral blood samples were provided by AllCells (PBMCs) or the UCLA Center for AIDS Research (CFAR) Virology Core Lab (peripheral blood). For whole blood, PBMCs were purified by Ficoll gradient using standard techniques. PurifieFull Paper
2011Doyon, Y;Vo, TD;Mendel, MC;Greenberg, SG;Wang, J;Xia, DF;Miller, JC;Urnov, FD;Gregory, PD;Holmes, MC;Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architecturesNature methods21131970K562 cells were transfected using the 96-well Nucleofector Kit SF (Lonza) per manufacturer's recommendations. PBMCs (AllCells) were received on ice, centrifuged at 1,000g for 5 min at room temperature (22 °C) and changed to pre-warmed RPMI medium supFull Paper
2011Chou, BK;Mali, P;Huang, X;Ye, Z;Dowey, SN;Resar, LM;Zou, C;Zhang, YA;Tong, J;Cheng, L;Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signaturesCell research21243013f Hematology in Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA 5Cell Therapy Center, Xuanwu Hospital and Capital Medical University, Beijing, China 6AllCells LLC, Emeryville, California, USA *The JohFull Paper
2011Lu, SJ;Li, F;Yin, H;Feng, Q;Kimbrel, EA;Hahm, E;Thon, JN;Wang, W;Italiano, JE;Cho, J;Lanza, R;Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living miceCell research21221130versity of Illinois-Chicago review board. Informed consent was provided according to the Declaration of Helsinki. In some experiments, human blood samples were also obtained from a commercial source (AllCells, Emeryville, CA, USA). For hESC-PLTs, culFull Paper
2011Kikushige, Y;Ishikawa, F;Miyamoto, T;Shima, T;Urata, S;Yoshimoto, G;Mori, Y;Iino, T;Yamauchi, T;Eto, T;Niiro, H;Iwasaki, H;Takenaka, K;Akashi, K;Self-renewing hematopoietic stem cell is the primary target in pathogenesis of human chronic lymphocytic leukemiaCancer cell21840488Human age-matched adult bone marrow and peripheral blood cells were obtained from healthy donors or purchased from AllCells Inc. (Emeryville, CA, USA). Informed consent was obtained from all patients and controls in accordance with the Helsinki DeclaFull Paper
2011Panuganti, S;Towards large-scale production of platelets for transfusion based on ex vivo expansion of megakaryocytes from hematopoietic stem cellsThesisCultures were initiated in T-flasks with previously frozen mPB CD34-selected cells (AllCells; Berkeley, CA or Fred Hutchinson Cancer Research Center; Seattle, WA) and maintained at a concentration of 100,000 to 300,000 cells/ml in X-VIVO 20 (Lonza; WFull Paper
2011Carter, CC;McNamara, LA;Onafuwa-Nuga, A;Shackleton, M;Riddell, J;Bixby, D;Savona, MR;Morrison, SJ;Collins, KL;HIV-1 utilizes the CXCR4 chemokine receptor to infect multipotent hematopoietic stem and progenitor cellsCell host & microbe21402361We obtained pre-existing umbilical cord blood lacking subject identifiers after scheduled cesarean section procedures. We obtained fresh whole bone marrow aspirates from a commercial source (AllCells Ltd.). We prepared BMMCs and UCB mononuclear cellsFull Paper
2011Matsuyama, H;Suzuki, HI;Nishimori, H;Noguchi, M;Yao, T;Komatsu, N;Mano, H;Sugimoto, K;Miyazono, K;miR-135b mediates NPM-ALK-driven oncogenicity and renders IL-17-producing immunophenotype to anaplastic large cell lymphomaBlood22042699Many transformed lymphoma cells show immune-phenotypes resembling the corresponding normal lymphocytes, which gives a guide for proper diagnosis and presents promising Save;Human normal peripheral blood pan T lymphocytes were purchased from AllCellFull Paper
2011Aguila, JR;Liao, W;Yang, J;Avila, C;Hagag, N;Senzel, L;Ma, Y;SALL4 is a robust stimulator for the expansion of hematopoietic stem cellsBlood21602528Normal human bone marrow CD34 + cells were purchased (AllCells) and single-cell suspensions were generated in StemSpan SFEM (StemCell Technologies) for 24 hours. Next, 10 5 SaveFull Paper
2011Iancu-Rubin, C;Gajzer, D;Tripodi, J;Najfeld, V;Gordon, RE;Hoffman, R;Atweh, GF;Down-regulation of stathmin expression is required for megakaryocyte maturation and platelet productionBlood21364187Human MKs were generated in liquid culture from peripheral blood (PB)-derived CD34 + cells purchased from AllCells and suspended in Iscove modified Dulbecco medium (Invitrogen) SaveFull Paper
2011Liu, ZJ;Italiano, J;Ferrer-Marin, F;Gutti, R;Bailey, M;Poterjoy, B;Rimsza, L;Sola-Visner, M;Developmental differences in megakaryocytopoiesis are associated with up-regulated TPO signaling through mTOR and elevated GATA-1 levels in neonatal megakaryocytesBlood21304100Human umbilical CB-CD34+ cells were purchased from AllCells. G-CSF-mobilized adult peripheral blood (PB) CD34+ cells were purchased from the Fred Hutchinson Cancer Institute (Seattle, WA). CD34+ cells from either source were cultured in serum-free meFull Paper
2011Adams, GN;LaRusch, GA;Stavrou, E;Zhou, Y;Nieman, MT;Jacobs, GH;Cui, Y;Lu, Y;Jain, MK;Mahdi, F;Shariat-Madar, Z;Okada, Y;D'Alecy, LG;Schmaier, AH;Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosisBlood21297000HUVECs were purchased from AllCells and grown on gelatin-coated plates in CSC media (Cells Systems). Cells were grown to near-confluence in 6-well plates and transfected with Lipofectamine 2000 reagent (Invitrogen) in OPTI-MEM media (Invitrogen) overFull Paper
2011Hu, K;Yu, J;Suknuntha, K;Tian, S;Montgomery, K;Choi, KD;Stewart, R;Thomson, JA;Slukvin, II;Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cellsBlood21296996Frozen CB mononuclear cells were obtained from AllCells. BM mononuclear cells from normal donors and from a patient with CML in the chronic phase were purchased from AllCells. SaveFull Paper
2011Karp, JE;Smith, BD;Resar, LS;Greer, JM;Blackford, A;Zhao, M;Moton-Nelson, D;Alino, K;Levis, MJ;Gore, SD;Joseph, B;Carraway, H;McDevitt, MA;Bagain, L;Mackey, K;Briel, J;Doyle, LA;Wright, JJ;Rudek, MA;Phase 1 and pharmacokinetic study of bolus-infusion flavopiridol followed by cytosine arabinoside and mitoxantrone for acute leukemiasBlood21239698Flavopiridol is a protein bound, cytotoxic, cyclin-dependent kinase inhibitor. Flavopiridol given by 1-hour bolus at 50 mg/m 2 daily 3 times followed by cytosine arabinoside and SaveFull Paper
2011Huang, X;Gschweng, E;Van Handel, B;Cheng, D;Mikkola, HK;Witte, ON;Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cellsBlood21163928The analyzed samples include H1 hESCs, day 7 and 15 H1 EB cells, and a mixture of 3 adult bone marrow CD34 + cells (AllCells). The data were analyzed with SDS RQ Manager 1.2 ( SaveFull Paper
2011Lugthart, S;Figueroa, ME;Bindels, E;Skrabanek, L;Valk, PJ;Li, Y;Meyer, S;Erpelinck-Verschueren, C;Greally, J;Löwenberg, B;Melnick, A;Delwel, R;Aberrant DNA hypermethylation signature in acute myeloid leukemia directed by EVI1Blood20855866healthy donors, 4 of which were acquired from the Translational Trials Development and Support Laboratory, Cincinnati Children's Hospital, and 4 of which were purchased from Allcells SaveFull Paper
2011Mack, AA;Kroboth, S;Rajesh, D;Wang, WB;Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectorsPloS one22132178a permit number or approval ID since mouse is not a protected species. Processing whole blood samples Peripheral (PB.1 and PB.2) and cord (CB.1 and CB.2) blood-derived CD34+ cells were obtained from AllCells (Emeryville, CA USA). Blood collections wFull Paper
2011Moorefield, EC;McKee, EE;Solchaga, L;Orlando, G;Yoo, JJ;Walker, S;Furth, ME;Bishop, CE;Cloned, CD117 selected human amniotic fluid stem cells are capable of modulating the immune responsePloS one22046303lates for Elispot (Millipore, Billerica, MA) were coated with human interferon-γ (IFN-γ) capture antibody (2G1; Endogen, Rockford, IL). Peripheral blood mononuclear cells (PBMCs) were acquired from AllCells (Emeryville, CA), activated with 5 µg/ml PHFull Paper
2011Corbin, AS;Agarwal, A;Loriaux, M;Cortes, J;Deininger, MW;Druker, BJ;Human chronic myeloid leukemia stem cells are insensitive to imatinib despite inhibition of BCR-ABL activityThe Journal of clinical investigation21157039Imatinib therapy, which targets the oncogene product BCR-ABL, has transformed chronic myeloid leukemia (CML) from a life-threatening disease into a chronic condition. Most patients, SaveFull Paper
2011Rowe, DD;Secreted factors from human umbilical cord blood cells protect oligodendrocytes from ischemic insultThesisthat prevented OL-HUCB cell contact but was permeable to media and soluble factors. Cryopreserved HUCB cells (ALLCELLS, Emeryville, CA) were rapidly thawed, washed, pelleted to remove the cryopreservatives and re-suspended in 10 ml DMEM with glucoseFull Paper
2011Zhou, L;McMahon, C;Bhagat, T;Alencar, C;Yu, Y;Fazzari, M;Sohal, D;Heuck, C;Gundabolu, K;Ng, C;Mo, Y;Shen, W;Wickrema, A;Kong, G;Friedman, E;Sokol, L;Mantzaris, I;Mantzaris, G;Pellagatti, A;Boultwood, J;Platanias, LC;Steidl, U;Yan, L;Yingling, JM;Lahn, MM;List, A;Bitzer, M;Verma, A;Reduced SMAD7 leads to overactivation of TGF-beta signaling in MDS that can be reversed by a specific inhibitor of TGF-beta receptor I kinaseCancer research21189329Bone marrow CD34+ cells from various normal donors were also obtained from AllCells (Emeryville, CA). K562 and HS-5 cell lines were purchased from ATCC. Erythropoietin and Transforming growth factor-β1 (TGF-β1) were bought from R&D Systems (MinneapolFull Paper
2011Nicolaides, TP;Li, H;Solomon, DA;Hariono, S;Hashizume, R;Barkovich, K;Baker, SJ;Paugh, BS;Jones, C;Forshew, T;Hindley, GF;Hodgson, JG;Kim, JS;Rowitch, DH;Weiss, WA;Waldman, TA;James, CD;Targeted therapy for BRAFV600E malignant astrocytomaClinical cancer research : an official journal of the American Association for Cancer Research22038996Normal human astrocytes (NHA) were obtained from Clonetics and AllCells. All cell sources were authenticated through DNA fingerprinting using the Promega Powerplex platform. SaveFull Paper
2011Kurtzberg, LS;Roth, S;Krumbholz, R;Crawford, J;Bormann, C;Dunham, S;Yao, M;Rouleau, C;Bagley, RG;Yu, XJ;Wang, F;Schmid, SM;Lavoie, EJ;Teicher, BA;Genz-644282, a novel non-camptothecin topoisomerase I inhibitor for cancer treatmentClinical cancer research : an official journal of the American Association for Cancer Research21415217Viable human bone marrow cells were purchased as frozen vials from AllCells, LLC., via StemCell Technologies (#ABM009). They were prepared with a DNase treatment (#07900; SaveFull Paper
2011Arastu-Kapur, S;Anderl, JL;Kraus, M;Parlati, F;Shenk, KD;Lee, SJ;Muchamuel, T;Bennett, MK;Driessen, C;Ball, AJ;Kirk, CJ;Nonproteasomal targets of the proteasome inhibitors bortezomib and carfilzomib: a link to clinical adverse eventsClinical cancer research : an official journal of the American Association for Cancer Research21364033Purpose: Bortezomib (Velcade), a dipeptide boronate 20S proteasome inhibitor and an approved treatment option for multiple myeloma, is associated with a treatment-emergent, painful SaveFull Paper
2011Chao, MP;Alizadeh, AA;Tang, C;Jan, M;Weissman-Tsukamoto, R;Zhao, F;Park, CY;Weissman, IL;Majeti, R;Therapeutic antibody targeting of CD47 eliminates human acute lymphoblastic leukemiaCancer research21177380Human normal bone marrow (NBM) cells were purchased from AllCells Inc. Human ALL samples were obtained from patients at the Stanford University Medical Center, with informed SaveFull Paper
2011Pang, WW;Price, EA;Sahoo, D;Beerman, I;Maloney, WJ;Rossi, DJ;Schrier, SL;Weissman, IL;Human bone marrow hematopoietic stem cells are increased in frequency and myeloid-biased with ageProceedings of the National Academy of Sciences of the United States of America22123971In the human hematopoietic system, aging is associated with decreased bone marrow cellularity, decreased adaptive immune system function, and increased incidence of anemia and Save;Normal young human bone marrow mononuclear cells were purchased froFull Paper
2011Jan, M;Chao, MP;Cha, AC;Alizadeh, AA;Gentles, AJ;Weissman, IL;Majeti, R;Prospective separation of normal and leukemic stem cells based on differential expression of TIM3, a human acute myeloid leukemia stem cell markerProceedings of the National Academy of Sciences of the United States of America21383193Normal human bone marrow mononuclear cells were purchased from AllCells. Human AML samples were obtained from patients at the Stanford Medical Center with informed consent, SaveFull Paper
2011Sahler, J;Bernard, JJ;Spinelli, SL;Blumberg, N;Phipps, RP;The Feverfew plant-derived compound, parthenolide enhances platelet production and attenuates platelet activation through NF-κB inhibitionThrombosis research21272923Adapted from previous procedures [4], human CD34+ cord blood cells were purchased from Allcells (Emeryville, CA) and first plated at 2.5 × 10 5 cells/mL in IMDM supplemented with SaveFull Paper
2011Nahimana, A;Aubry, D;Lagopoulos, L;Greaney, P;Attinger, A;Demotz, S;Dawson, KM;Schapira, M;Tschopp, J;Dupuis, M;Duchosal, MA;A novel potent Fas agonist for selective depletion of tumor cells in hematopoietic transplantsBlood cancer journal22829095lture and analysis conditions Human CD34+ cells from cord blood (CB), mobilized peripheral blood (mPB) or bone marrow (BM) were obtained unpurified (mPB) or already purified (CB, mPB, BM) from either AllCells, LLC (San Mateo, CA, USA), or purified usFull Paper
2011Hart, S;Goh, KC;Novotny-Diermayr, V;Tan, YC;Madan, B;Amalini, C;Ong, LC;Kheng, B;Cheong, A;Zhou, J;Chng, WJ;Wood, JM;Pacritinib (SB1518), a JAK2/FLT3 inhibitor for the treatment of acute myeloid leukemiaBlood cancer journal22829080Primary cells, either peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex ( SaveFull Paper
2011Nagao, R;Ashihara, E;Kimura, S;Strovel, JW;Yao, H;Takeuchi, M;Tanaka, R;Hayashi, Y;Hirai, H;Padia, J;Strand, K;Maekawa, T;Growth inhibition of imatinib-resistant CML cells with the T315I mutation and hypoxia-adaptation by AV65--a novel Wnt/β-catenin signaling inhibitorCancer letters21906872Bone marrow (BM) mononuclear cells obtained from CML patients and healthy volunteers (ALLCells, Emeryville, CA) were plated in duplicate in MethoCult H4434 Classic (StemCell Technologies Inc, Vancouver, Canada) and cultured at 37 °C in 5% CO2. AfterFull Paper
2011Gao, N;Budhraja, A;Cheng, S;Liu, EH;Chen, J;Yang, Z;Chen, D;Zhang, Z;Shi, X;Phenethyl isothiocyanate exhibits antileukemic activity in vitro and in vivo by inactivation of Akt and activation of JNK pathwaysCell death & disease21472003Fresh normal peripheral blood mononuclear cells were purchased from Allcells (Emeryvill, CA, USA). After washing and enumerating, cells were suspended at 8 × 10 5 /ml before SaveFull Paper
2011Yang, L;Wang, H;Kornblau, SM;Graber, DA;Zhang, N;Matthews, JA;Wang, M;Weber, DM;Thomas, SK;Shah, JJ;Zhang, L;Lu, G;Zhao, M;Muddasani, R;Yoo, SY;Baggerly, KA;Orlowski, RZ;Evidence of a role for the novel zinc-finger transcription factor ZKSCAN3 in modulating Cyclin D2 expression in multiple myelomaOncogene21057542The current studies on deidentified aliquots were performed according to an Institutional Review Board-approved protocol. Normal human CD19+ B-cell total RNA was from Miltenyi Biotec, Inc., and plasma cell cDNA was from AllCells LLC. (Berkeley, CA)Full Paper
2011Yu, X;McCarthy, PJ;Lim, HJ;Iempridee, T;Kraus, RJ;Gorlen, DA;Mertz, JE;The ZIIR element of the Epstein-Barr virus BZLF1 promoter plays a central role in establishment and maintenance of viral latencyJournal of virology21389123Fresh human primary B cells from peripheral blood of healthy donors were purchased from AllCells (Emeryville, CA). The B cells were infected the day they arrived by incubation at 4C SaveFull Paper
2011Sagawa, M;Shimizu, T;Fukushima, N;Kinoshita, Y;Ohizumi, I;Uno, S;Kikuchi, Y;Ikeda, Y;Yamada-Okabe, H;Kizaki, M;A new disulfide-linked dimer of a single-chain antibody fragment against human CD47 induces apoptosis in lymphoid malignant cells via the hypoxia inducible factor-1α pathwayCancer science21401803CD47 belongs to the immunoglobulin superfamily and is associated with β‐integrins. Recently it was reported that CD47 ligation rapidly induces apoptosis in B‐chronic lymphocytic SaveFull Paper
2011Takeuchi, M;Ashihara, E;Yamazaki, Y;Kimura, S;Nakagawa, Y;Tanaka, R;Yao, H;Nagao, R;Hayashi, Y;Hirai, H;Maekawa, T;Rakicidin A effectively induces apoptosis in hypoxia adapted Bcr-Abl positive leukemic cellsCancer science21166958Bone marrow cells from two normal volunteers and CML patients in the chronic phase before imatinib treatment were purchased from AllCells Inc. (Emeryville, CA, USA). Thawed SaveFull Paper
2011Su, WR;Zhang, QZ;Shi, SH;Nguyen, AL;Le, AD;Human gingiva-derived mesenchymal stromal cells attenuate contact hypersensitivity via prostaglandin E2-dependent mechanismsStem cells (Dayton, Ohio)21987520CD14+ cells were negatively selected from human peripheral blood mononuclear cells (PBMCs) (AllCells LLC, Emeryville, CA, http://www.allcells.com) using the MACS CD14 MicroBeads (Monocyte Isolation Kit, Miltenyi Biotec, Auburn, CA, http://www.miltenyFull Paper
2011Bissels, U;Wild, S;Tomiuk, S;Hafner, M;Scheel, H;Mihailovic, A;Choi, YH;Tuschl, T;Bosio, A;Combined characterization of microRNA and mRNA profiles delineates early differentiation pathways of CD133+ and CD34+ hematopoietic stem and progenitor cellsStem cells (Dayton, Ohio)21394831tenyi Biotec) using the negative fraction of the first separation. The gating of the separated cells was performed according to ISHAGE. CD133+ cells from mobilized peripheral blood were obtained from AllCells LLC. RNA Sources Total RNA was isolatedFull Paper
2011Mendelson, A;Frank, E;Allred, C;Jones, E;Chen, M;Zhao, W;Mao, JJ;Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cells in vitroFASEB journal : official publication of the Federation of American Societies for Experimental Biology21746864Human bone marrow MSCs (AllCells, Emeryville, CA, USA), human ASCs, and human SSCs were isolated from fresh adult donor samples using previously established methods (27-31). MSCs were purified from bone marrow using the RosetteSep Human Mesenchymal SFull Paper
2011Gao, N;Budhraja, A;Cheng, S;Liu, EH;Huang, C;Chen, J;Yang, Z;Chen, D;Zhang, Z;Shi, X;Interruption of the MEK/ERK signaling cascade promotes dihydroartemisinin-induced apoptosis in vitro and in vivoApoptosis : an international journal on programmed cell death21336837Fresh normal peripheral blood mononuclear cells were purchased from Allcells (Emeryville, CA, USA). After washing and enumerating as described for mononuclear cells, cells were SaveFull Paper
2011Zhou, L;Opalinska, J;Sohal, D;Yu, Y;Mo, Y;Bhagat, T;Abdel-Wahab, O;Fazzari, M;Figueroa, M;Alencar, C;Zhang, J;Kambhampati, S;Parmar, S;Nischal, S;Hueck, C;Suzuki, M;Freidman, E;Pellagatti, A;Boultwood, J;Steidl, U;Sauthararajah, Y;Yajnik, V;McMahon, C;Gore, SD;Platanias, LC;Levine, R;Melnick, A;Wickrema, A;Greally, JM;Verma, A;Aberrant epigenetic and genetic marks are seen in myelodysplastic leukocytes and reveal Dock4 as a candidate pathogenic gene on chromosome 7qThe Journal of biological chemistry21532034106 CD34+ cells (AllCells) were thawed, cultured for 2 h, resuspended in 100 μl of human CD34+ Nucleofection solution (Amaxa), then transferred into cuvettes, and electroporated using program U008. CD34+ cells were collected and cultured in 24-well pFull Paper
2011Froelich, S;Tai, A;Kennedy, K;Zubair, A;Wang, P;Pseudotyping lentiviral vectors with aura virus envelope glycoproteins for DC-SIGN-mediated transduction of dendritic cellsHuman gene therapy21452926Peripheral blood mononuclear cells (PBMCs) from healthy human donors were purchased from AllCells. PBMCs were allowed to adhere for 2 hr on non-tissue culture-treated 24-well plates and washed extensively. Adherent monocytes were plated at a densityFull Paper
2011Jiang, X;Christopherson, GT;Mao, HQ;The effect of nanofibre surface amine density and conjugate structure on the adhesion and proliferation of human haematopoietic progenitor cellsInterface focus23050077Frozen human UCB CD133+ cells were purchased from AllCells, LLC (Emeryville, CA, USA). The CD133+ purity in the post-thawed HSPCs was determined by flow cytometry to be 97 per cent and the viability was determined by Trypan blue exclusion assay to beFull Paper
2011Zhu, J;Shimizu, E;Zhang, X;Partridge, NC;Qin, L;EGFR signaling suppresses osteoblast differentiation and inhibits expression of master osteoblastic transcription factors Runx2 and OsterixJournal of cellular biochemistry21381079The epidermal growth factor receptor (EGFR) and its ligands regulate key processes of cell biology, such as proliferation, survival, differentiation, migration, and tumorigenesis. We SaveFull Paper
2011Khoury, M;Drake, A;Chen, Q;Dong, D;Leskov, I;Fragoso, MF;Li, Y;Iliopoulou, BP;Hwang, W;Lodish, HF;Chen, J;Mesenchymal stem cells secreting angiopoietin-like-5 support efficient expansion of human hematopoietic stem cells without compromising their repopulating potentialStem cells and development21142526Purified human CD133 + cord blood cells were purchased from AllCells. Alternatively, umbilical cord blood was obtained from the National Disease Research Interchange or the SaveFull Paper
2011Hong, L;Zhang, G;Sultana, H;Yu, Y;Wei, Z;The effects of 17-β estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitroStem cells and development20735179Fresh human bone marrow donated by 12 healthy donors (6 males and 6 females, average age = 27.4 ± 6.1) were purchased (Allcells, Emeryville, CA). Mononuclear cells were isolated using density gradient centrifugation (Histopaque-1.077; Sigma, St. LouiFull Paper
2011Kunisato, A;Wakatsuki, M;Shinba, H;Ota, T;Ishida, I;Nagao, K;Direct generation of induced pluripotent stem cells from human nonmobilized bloodStem cells and development20497033BM and PB MNCs were obtained from Allcells (ABM007F and PB003F each). Frozen BM MNCs purchased from Allcells were collected from a 25-year-old male donor (lot#BM1705), a SaveFull Paper
2011Basu, J;Genheimer, CW;Sangha, N;Quinlan, SF;Guthrie, KI;Kelley, R;Ilagan, RM;Jain, D;Bertram, T;Ludlow, JW;Organ specific regenerative markers in peri-organ adipose: kidneyLipids in health and disease21957910CD34+ GCSF mobilized/non-mobilized peripheral blood mononuclear cell cDNA was purchased from AllCells LLC. Fetal and adult hepatocyte cDNA and keratinocyte cDNA were SaveFull Paper
2011Gupta, MS;Cooper, ES;Nicoll, SB;Transforming growth factor-beta 3 stimulates cartilage matrix elaboration by human marrow-derived stromal cells encapsulated in photocrosslinked carboxymethylcellulose hydrogels: potential for nucleus pulposus replacementTissue engineering. Part A21707438hMSCs were isolated from the bone marrow of a 37-year-old man (AllCells, Emeryville, CA) using enriched density centrifugation. Briefly, an hMSC Enrichment Cocktail (StemCell Technologies, Vancouver, BC, Canada)44 was added to the bone marrow and incFull Paper
2011McWhirter, J;Noguchi, H;Gompper, G;Deformation and clustering of red blood cells in microcapillary flowsSoft MatterTrajectories are shown in a co-moving frame, ie relative to the center-of-mass of allcells. Solid and dashed lines represent the cm of RBCs and their periodic images, respectively. SaveFull Paper
2011Vanella, L;Kim, DH;Sodhi, K;Barbagallo, I;Burgess, AP;Falck, JR;Schwartzman, ML;Abraham, NG;Crosstalk between EET and HO-1 downregulates Bach1 and adipogenic marker expression in mesenchymal stem cell derived adipocytesProstaglandins & other lipid mediators21821145Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 10% heat inactivated fetal boviFull Paper
2011MacEneaney, OJ;Connick, E;DeSouza, CA;Effects of HIV-1 gp120 and protease inhibitors on apoptotic susceptibility of CD34+ hematopoietic progenitor cellsJournal of acquired immune deficiency syndromes (1999)21233630Freshly-isolated CD34+ cells from healthy G-CSF-treated human donors (n=3) were obtained from an independent supplier (Allcells, LLC, Berkeley, CA). Donors tested negative for HIV-1, hepatitis B and hepatitis C. Viability of cells determined by trypaFull Paper
2011He, JQ;Vu, DM;Hunt, G;Chugh, A;Bhatnagar, A;Bolli, R;Human cardiac stem cells isolated from atrial appendages stably express c-kitPloS one22140461Human bone marrow cells were purchased from AllCells (Emeryville, CA). Total RNA of human carotid artery was bought from Agilent Technologies (Santa Clara, CA). Cells were SaveFull Paper
2011Kobayashi, DT;Olson, RJ;Sly, L;Swanson, CJ;Chung, B;Naryshkin, N;Narasimhan, J;Bhattacharyya, A;Mullenix, M;Chen, KS;Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analysesPloS one21904622For the dilutional linearity experiment, six lots of control PBMCs were purchased from AllCells for further assay testing. After thawing and lysis, PBMCs were serially diluted 4-fold and SaveFull Paper
2011Froelich, S;Tai, A;Kennedy, K;Zubair, A;Wang, P;Virus-receptor mediated transduction of dendritic cells by lentiviruses enveloped with glycoproteins derived from Semliki Forest virusPloS one21738680th 293T.DCSIGN cells for 8 hrs before changing to fresh D10 medium. Transduction of human PBMC-derived DCs Peripheral blood mononuclear cells (PBMC) from two healthy human donors were purchased from AllCells (Emeryville, CA). PBMC were differentiateFull Paper
2011Danner, R;Chaudhari, SN;Rosenberger, J;Surls, J;Richie, TL;Brumeanu, TD;Casares, S;Expression of HLA class II molecules in humanized NOD.Rag1KO.IL2RgcKO mice is critical for development and function of human T and B cellsPloS one21611197) Tg.NOD.Rag1KO.IL2RγcKO (referred as DRAG) mice were maintained in the pathogen-free WRAIR Veterinary Medicine Facility. Infusion of mice with HSC Umbilical cord bloods (UCB) were purchased from AllCells (Emeryville,CA) and Promocell (Heidelberg, GFull Paper
2011Drake, AC;Khoury, M;Leskov, I;Iliopoulou, BP;Fragoso, M;Lodish, H;Chen, J;Human CD34+ CD133+ hematopoietic stem cells cultured with growth factors including Angptl5 efficiently engraft adult NOD-SCID Il2rγ-/- (NSG) micePloS one21559522rch. The MIT committee on animal care (MIT CAC) approved the research as part of animal protocol 0310-027-13. Cell isolation and culture Purified CD133+ human umbilical cord HSCs were purchased from AllCells (California) or National Disease ResearchFull Paper
2011Burridge, PW;Thompson, S;Millrod, MA;Weinberg, S;Yuan, X;Peters, A;Mahairaki, V;Koliatsos, VE;Tung, L;Zambidis, ET;A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variabilityPloS one21494607For generation of the non-viral CD34+ cord blood-derived hiPSC lines (CBiPSC6.2, CBiPSC6.11, CBiPSC6.13 and CBiPSC19.11), 0.5-1×106 human CD34+ cord blood cells (AllCells, Emeryville, CA, http://www.allcells.com) were expanded in StemSpan-SFEMFull Paper
2011Martellini, JA;Cole, AL;Svoboda, P;Stuchlik, O;Chen, LM;Chai, KX;Gangrade, BK;Sørensen, OE;Pohl, J;Cole, AM;HIV-1 enhancing effect of prostatic acid phosphatase peptides is reduced in human seminal plasmaPloS one21283773obtained from the National Institutes of Health AIDS Research and Reference Reagent Program (Germantown, MD, USA), and Peripheral Blood Mononuclear Cells (PBMCs) were obtained from healthy donors by AllCells, LLC (Emeryville, CA, USA). TZM-bl cells wFull Paper
2011Gupta, A;Le, A;Belinka, BA;Kachlany, SC;In vitro synergism between LFA-1 targeting leukotoxin (Leukothera™) and standard chemotherapeutic agents in leukemia cellsLeukemia research21664691and maintained in RPMI 1640 medium with 10% fetal bovine serum (Invitrogen, Carlsbad, CA) at 37 °C, 5% CO2. 2.2. Primary cells Frozen primary human cells were purchased from AllCells, LLC. (Emeryville, CA). Viability of these primary cells was 90%Full Paper
2011Bouchez, LC;Boitano, AE;de Lichtervelde, L;Romeo, R;Cooke, MP;Schultz, PG;Small-molecule regulators of human stem cell self-renewalChembiochem : a European journal of chemical biology21381174screen: The assay was performed on purified human CD34 + cells from granulocyte colony-stimulating factor-mobilized peripheral blood from normal donors (supplied by AllCells, SaveFull Paper
2011Fukumoto, R;Kiang, JG;Geldanamycin analog 17-DMAG limits apoptosis in human peripheral blood cells by inhibition of p53 activation and its interaction with heat-shock protein 90 kDa after exposure to ionizing radiationRadiation research21663398Exposure to ionizing radiation induces p53, and its inhibition improves mouse survival. We tested the effect of 17-dimethylamino-ethylamino-17-demethoxygeldanamycin (17-DMAG) on SaveFull Paper
2011Pogosova-Agadjanyan, EL;Fan, W;Georges, GE;Schwartz, JL;Kepler, CM;Lee, H;Suchanek, AL;Cronk, MR;Brumbaugh, A;Engel, JH;Yukawa, M;Zhao, LP;Heimfeld, S;Stirewalt, DL;Identification of radiation-induced expression changes in nonimmortalized human T cellsRadiation research21268710Peripheral blood samples were collected from healthy volunteer donors at the Fred Hutchinson Cancer Research Center (FHCRC) or purchased from a commercial vendor (AllCells, Emeryville, CA). All samples were obtained under Institutional Review Board (Full Paper
2011Bagley, RG;Kurtzberg, L;Rouleau, C;Yao, M;Teicher, BA;Erufosine, an alkylphosphocholine, with differential toxicity to human cancer cells and bone marrow cellsCancer chemotherapy and pharmacology21526352and compounds were added to each well of a 6-well plate in a final volume of 3 mls; RPMI8226 multiple myeloma cells and compounds were added to 3 mls Methocult medium (AllCells SaveFull Paper
2011Hong, L;Krishnamachari, Y;Seabold, D;Joshi, V;Schneider, G;Salem, AK;Intracellular release of 17-β estradiol from cationic polyamidoamine dendrimer surface-modified poly (lactic-co-glycolic acid) microparticles improves osteogenic differentiation of human mesenchymal stromal cellsTissue engineering. Part C, Methods20883116and solvents were purchased from Sigma. All the solvents, including ethyl acetate and dichloromethane (DCM) and acetonitrile, were of high performance liquid chromatography (HPLC) analytical grade. Fresh human bone marrow was purchased from Allcells.Full Paper
2011McNeish, J;Mathieu, A;Dempsey, B;Book Review: Bringing stem cell science to the classroomsRegenerative Medicinefrom a small biotechnology company, ReachBio (Seatttle, WA, USA). ReachBio is a contract of clients", so education is clearly a passion at ReachBio. As much of the focus of the text is SaveFull Paper
2011Nelson, DM;Joseph, B;Hillion, J;Segal, J;Karp, JE;Resar, LM;Flavopiridol induces BCL-2 expression and represses oncogenic transcription factors in leukemic blasts from adults with refractory acute myeloid leukemiaLeukemia & lymphoma21728742from the peripheral blood blasts were compared to gene expression in CD34+ hematopoietic stem cells (HSCs); mRNA levels from the HSCs were arbitrarily assigned a value of 1.0. HSC RNA was obtained from a commercial source (ALLCELLS, CA, USA)Full Paper
2011Ma, K;Fox, L;Shi, G;Shen, J;Liu, Q;Pappas, JD;Cheng, J;Qu, T;Generation of neural stem cell-like cells from bone marrow-derived human mesenchymal stem cellsNeurological research22196762The hMSCs from four donors were obtained from AllCells LLC (Emeryville, CA, USA) and Cambrex Bioscience (Walkersville, MD, USA). These hMSCs are negative for surface markers associated with hematopoietic cells (e.g. CD11b, CD33, CD34, and CD133) andFull Paper
2011Wang, DY;Chang, CW;Lagacé, RE;Oldroyd, NJ;Hennessy, LK;Development and validation of the AmpFℓSTR® Identifiler® Direct PCR Amplification Kit: a multiplex assay for the direct amplification of single-source samplesJournal of forensic sciences21418220the performance of the Identifiler Direct Kit on buccal samples deposited on Indicating FTA cards, white blood cells (WBCs) from three individuals were purchased from AllCells, SaveFull Paper
2011Shah, BS;Mao, JJ;Labeling of mesenchymal stem cells with bioconjugated quantum dotsMethods in molecular biology (Clifton, N.J.)21153373Bone marrow samples from healthy human donors (AllCells, Berkeley, CA) 2. RosetteSepTM (Stem Cell Technologies, Vancouver, BC, Canada) 3. 35-mm petri dishes (Falcon, Becton Dickinson Labware, Franklin Lakes, NJ) 4. DMEM: Dulbecco’s Modified Eagle’Full Paper
2011Li, Z;Chen, J;In vitro functional study of miR-126 in leukemiaMethods in molecular biology (Clifton, N.J.)20931398Genomic DNA template isolated from human normal bone marrow mononuclear cells purchased from AllCells (Emeryville, CA). 3. MSCVpuro (Clontech, Mountain View, CA) is a retroviral vector for cloning and gene expression. 4. AML1-ETO fusion gene in a vFull Paper
2011Kanjevac, T;Milovanovic, M;Volarevic, V;Arsenijevic, N;Fluoride release from glass ionomer cements correlates with the necrotic death of human dental pulp stem cellsSerbian Journal of Experimental and Clinical ResearchHuman SHEDs were purchesed from AllCells (Emeryville, California USA). The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS, 100 IU/mL penicillin G and 100 μg/mL streptomycinFull Paper
2011Bassan, R;Rohatiner, A;Lerede, T;Carter, M;Di Bona, E;Pogliani, E;Rossi, G;Fabris, P;Morandi, S;Lambertenghi-Deliliers, G;Casula, P;Vespignani, M;Lister, T;Barbui, T;Early Application of Anthracyclines in Adult Acute Lymphoblastic Leukemia (ALL)Haematology and Blood Transfusion / Hämatologie und BluttransfusionHowever, because IDR resistance may be dose-dependent, as ascertained by in vitro studies on MDRl+ ALLcells [17], increaseddose IDR may find a place in the treatment of patients SaveFull Paper
2011Larson, R;Dodge, R;Szatrowski, T;Frankel, S;Results of Recent Clinical Trials in Acute Lymphoblastic Leukemia by the Cancer and Leukemia Group BHaematology and Blood Transfusion / Hämatologie und BluttransfusionRapid depletion of asparagine results in selective killing of ALLcells since normal cells are able to synthesize asparagine. Because asparaginase is not markedly myelosuppressive, it is SaveFull Paper
2011Bissels, U;Combined analysis of microRNA and mRNA signatures in human hematopoietic stem and progenitor cells using a novel microarray quantification systemThesisGranulocyte-colony stimulating factor (G-CSF) mobilized peripheral blood was obtained from healthy donors after informed consent (Allcells, Berkley, USA) and CD133+ cells were SaveFull Paper
2011Milewski, ME;The Angiogenic effect of Erythropoietin on Stem Cells In-VitroThesisHUVEC cells (p-10) were obtained from Allcells, LLC (Emeryville, CA). The cells were removed from the -80°C and placed in a 37°C water bath until thawed. 10 ml of HUVEC media (Allcells, Emeryville, CA) was added to the cells and centrifuged for 5 minFull Paper
2011Stewart, HRA;A novel reporter system where gene knockdown is used to assay intercellular siRNA transferThesisMesenchymal stem cells were harvested from bone marrow aspirates purchased from Lonza and AllCells. To isolate human MSCs, nucleated cells were recovered from the 10ml bone marrow aspirates with a density gradient (Ficoll-Paque) and resuspended in coFull Paper
2011KC, P;Proteomic Analysis of Human Mesenchymal Stem Cell Aging during serial sub-cultureThesisHuman bone marrow was purchased from AllCells, LLC, Emeryvill, CA from a 28 years old donor. Human mesenchymal stem cells were isolated following RosetteSep method and expanded in culture using CFU-F assay. Fluorescence-activated cell sorting (FACS)Full Paper
2011Stosich, MS;Micro-CT evaluation of MSC/HUVEC/HSC-mediated angiogenesis in large PCL-βTCP constructs implanted in nude miceThesisFor in vivo study, fresh bone marrow samples of anonymous adult male donors were purchased from AllCells (Berkeley, CA), and used to isolate human mesenchymal stem cells ( SaveFull Paper
2011Pang, WW;Park, CY;Greenberg, PL;Separation of normal and aberrant hematopoietic stem cells in myelodysplastic syndrome by CD69 expressionThesisHuman Samples. Normal young human bone marrow mononuclear cells were purchased from AllCells, Inc. (Emeryville, CA, USA). Normal young and old human bone marrow samples were obtained from hematologically normal donors at the Stanford Medical Center wFull Paper
2011Ramos, JH;Phenotypic and molecular characterization of a human adult bone marrow committed T cell progenitorThesisHuman mononuclear cells obtained from fresh, normal adult bone marrow were purchased from AllCells, LLC. (Emeryville, CA). Donors were between the ages of 18 and 55 years, 110 lbs, in good health, and screened for HIV, Hepatitis B and Hepatitis C befFull Paper
2011Thacker, SG;Type I Interferons and the Development of Impaired Vascular Function and Repair in Human and Murine LupusThesislU/ml for 6 hours, followed by RNA harvesting. Similar experiments were performed with a more purified EPC population which was directly obtained from control bone marrow. In brief, CD133+ cells obtained from healthy control bone marrows (AllCells,Full Paper
2011Panas, MW;The identification and elimination of immunosuppressive Mycobacterium bovis BCG genes to create novel rBCG vectors that generate increased CD8+ T cell responsesThesisThe delivery of an allelic exchange substrate to allcells within a mycobacterial population will be accomplished through specializedtransduction using the temperature sensitive phage SaveFull Paper
2011Hermann, RJ;Investigation of Ikaros-dependent transcriptional regulation of novel VPAC1 splice variants during epigenetic transformationThesisIn order to identify which PBMC were the cell source for smaller VPAC1 isoforms, purified samples of different cell populations that compose the collective PBMC pool had to be analyzed. cDNA from purified peripheral blood cell populations was purchasFull Paper
2011Furman, PA;Murakami, E;Niu, C;Lam, AM;Espiritu, C;Bansal, S;Bao, H;Tolstykh, T;Micolochick Steuer, H;Keilman, M;Zennou, V;Bourne, N;Veselenak, RL;Chang, W;Ross, BS;Du, J;Otto, MJ;Sofia, MJ;Activity and the metabolic activation pathway of the potent and selective hepatitis C virus pronucleotide inhibitor PSI-353661Antiviral research21600932The human bone marrow stem cell toxicity assay was performed by Reachbio (Seattle, WA). Clonogenic progenitor cells of the erythroid (CFU-E and BFU-E) and myeloid (CFU-GM) SaveFull Paper
2011Lam, AM;Espiritu, C;Murakami, E;Zennou, V;Bansal, S;Micolochick Steuer, HM;Niu, C;Keilman, M;Bao, H;Bourne, N;Veselenak, RL;Reddy, PG;Chang, W;Du, J;Nagarathnam, D;Sofia, MJ;Otto, MJ;Furman, PA;Inhibition of hepatitis C virus replicon RNA synthesis by PSI-352938, a cyclic phosphate prodrug of β-D-2'-deoxy-2'-α-fluoro-2'-β-C-methylguanosineAntimicrobial agents and chemotherapy21444700marrow (Lonza, Walkersville, MD) were performed by Reachbio (Seattle, WA) using a semisolid We also thank Emer Clarke at ReachBio LLC (Seattle, WA) for performing human bone SaveFull Paper
2011Veeranki, S;Duan, X;Panchanathan, R;Liu, H;Choubey, D;IFI16 protein mediates the anti-inflammatory actions of the type-I interferons through suppression of activation of caspase-1 by inflammasomesPloS one22046441y the AIM2 and NLRP3 inflammasomes. Materials and Methods Cells and Treatments Frozen human peripheral blood mononuclear cells (PBMCs), purified CD14+ monocytes and CD19+ B cells were purchased from ReachBio (Seattle, WA). THP-1 cells were purchasedFull Paper
2011Maier, MS;Setting occupational exposure limits for unstudied pharmaceutical intermediates using an in vitro parallelogram approachToxicology mechanisms and methods20156007The mean ± 1 SD was calculated for triplicate cultures. The laboratory (ReachBio LLC, Seattle, Washington) was blinded to the identify of the test compounds and docetaxel served as the positive control, with blank and vehicle as negative controlsFull Paper
2011Lautenschlager, F;Cell compliance: cytoskeletal origin and importance for cellular function.ThesisHuman MSCs (hMSCs) were investigated over the time they needed to double their population 17 times, which corresponds to about eight passages. Cells were obtained from the bone marrow of adult donors (Stem Cell Technologies and ReachBio LLC), isolateFull Paper
2010Yelamanchili, SV;Chaudhuri, AD;Chen, LN;Xiong, H;Fox, HS;MicroRNA-21 dysregulates the expression of MEF2C in neurons in monkey and human SIV/HIV neurological diseaseCell Death Dis21170291miRNA in situ hybridization. Following necropsy, monkey caudate and hippocampus were formalin fixed, and embedded in paraffin, and 5 μm sections cut onto glass slides. The in situ hybridization was performed at Phylogeny Inc. (Columbus, OH, USA) as dFull Paper
2010Maret, D;Gruzglin, E;Sadr, MS;Siu, V;Shan, W;Koch, AW;Seidah, NG;Del Maestro, RF;Colman, DR;Surface expression of precursor N-cadherin promotes tumor cell invasionNeoplasia21170270Melanoma, breast, squamous cell, and prostate carcinoma tissue samples and arrays, in addition to corresponding normal tissue, were obtained from FolioBio (Columbus, OH). Metastatic melanoma arrays were from Imgenex (San Diego, CA). Cell Culture andFull Paper
2010Teichert, A;Elalieh, H;Bikle, D;Disruption of the hedgehog signaling pathway contributes to the hair follicle cycling deficiency in Vdr knockout miceJ. Cell. Physiol.20458748The binding of the antibodies was detected by biotinylated goat anti‐rabbit IgG, followed by ABC‐peroxidase reagent (Vector, Burlingame, CA) and revealed with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followed by hematoxyliFull Paper
2010Kashyap, MK;Harsha, HC;Renuse, S;Pawar, H;Sahasrabuddhe, NA;Kim, MS;Marimuthu, A;Keerthikumar, S;Muthusamy, B;Kandasamy, K;Subbannayya, Y;Prasad, TS;Mahmood, R;Chaerkady, R;Meltzer, SJ;Kumar, RV;Rustgi, AK;Pandey, A;SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretomeCancer Biol. Ther.20686364... _id":"553574440","term_text":"ESC96101"}}ESC96101 [/protein/ESC96101]) consisted of 34 cases in duplicate, ranging from differentiation grades I–III from patient's ages 36–71 years. The TMAs from FolioBio (catalog # ARY-HH0091) consisted of 40 ESFull Paper
2010Li, Z;Qi, CF;Shin, DM;Zingone, A;Newbery, HJ;Kovalchuk, AL;Abbott, CM;Morse, HC;Eef1a2 promotes cell growth, inhibits apoptosis and activates JAK/STAT and AKT signaling in mouse plasmacytomasPLoS ONE20505761correction. A tissue microarray of costal biopsies of normal individuals or patients diagnosed with MM was purchased from Folio BioSciences (Columbus, OH). Immunohistochemical studies were detailed previously [27]. MouseFull Paper
2010Shih, DM;Xia, YR;Yu, JM;Lusis, AJ;Temporal and tissue-specific patterns of Pon3 expression in mouse: in situ hybridization analysisAdv. Exp. Med. Biol.20221872Detailed biochemical studies are also necessary to identify the physiological substrate(s) of PON3 in various cells/tissues. Notes. Acknowledgment. We thank Yi-Shou Shi, XuPing Wang, and Phylogeny, Inc. (Columbus, OH) for excellent technical supportFull Paper
2010Hill, LS;Richardson, TL;Profeta, LT;Shaw, TJ;Hintz, CJ;Twining, BS;Lawrenz, E;Myrick, ML;Construction, figures of merit, and testing of a single-cell fluorescence excitation spectroscopy systemRev Sci Instrum20113077The relay box uses a Bud Industries PN series box enclosure (model PN-1341-C) as the base. The circuit consisted of two NTE Electronics solid-state relays (model RS3–1D10–51), two Omron Electronic relays (model G4B-112T-C-U.S.-AC120), one Qualtek EleFull Paper
2010Gentles, AJ;Plevritis, SK;Majeti, R;Alizadeh, AA;Association of a leukemic stem cell gene expression signature with clinical outcomes in acute myeloid leukemiaJAMA21177505Normal human bone marrow mononuclear cells were purchased from AllCells Inc (Emeryville, California), and human cord blood was obtained from Stanford University Medical Center. SaveFull Paper
2010Carter, CC;Onafuwa-Nuga, A;McNamara, LA;Riddell, J;Bixby, D;Savona, MR;Collins, KL;HIV-1 infects multipotent progenitor cells causing cell death and establishing latent cellular reservoirsNature medicine20208541minates all consensus AP-1, NF-AT, NF-κB SP-1 and TATA motifs, but maintains the polyA site found in the R region. Cell culture Fresh whole bone marrow aspirates were obtained commercially (AllCells ltd.). Pre-existing umbilical cord blood lacking pFull Paper
2010Naka, K;Hoshii, T;Muraguchi, T;Tadokoro, Y;Ooshio, T;Kondo, Y;Nakao, S;Motoyama, N;Hirao, A;TGF-beta-FOXO signalling maintains leukaemia-initiating cells in chronic myeloid leukaemiaNature20130650solid medium. Human LICs were purified as CD34+CD38-Lin- cells of bone marrow cells from CML patients (Cureline, Inc. and AllCells). These cells were co-cultured on OP-9 cells for 5 days before transfer to semi-solid medium. Cell cycling was assessedFull Paper
2010Lishko, PV;Botchkina, IL;Fedorenko, A;Kirichok, Y;Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channelCell20144758Frozen normal peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryville, CA), thawed and plated onto sterile coverslips according to the manufacturer instructions. Mouse monoclonal anti-COXIV and anti-CD14 antibody were fromFull Paper
2010Loewer, S;Cabili, MN;Guttman, M;Loh, YH;Thomas, K;Park, IH;Garber, M;Curran, M;Onder, T;Agarwal, S;Manos, PD;Datta, S;Lander, ES;Schlaeger, TM;Daley, GQ;Rinn, JL;Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cellsNature genetics21057500The CD34 + cells used were mobilized peripheral blood CD34+ cells (AllCells, mBP014F). Cells were thawed and cultured as described 21 for two days before RNA isolation. SaveFull Paper
2010Pichiorri, F;Suh, SS;Rocci, A;De Luca, L;Taccioli, C;Santhanam, R;Zhou, W;Benson, DM;Hofmainster, C;Alder, H;Garofalo, M;Di Leva, G;Volinia, S;Lin, HJ;Perrotti, D;Kuehl, M;Aqeilan, RI;Palumbo, A;Croce, CM;Downregulation of p53-inducible microRNAs 192, 194, and 215 impairs the p53/MDM2 autoregulatory loop in multiple myeloma developmentCancer cell20951946IRB-approved procurement protocol (2000C0247) at The Ohio State University and The University of Turin GIMEMA-MM-03-05, N. EUDRACT 2005-004745-33). Three of the five healthy PCs used were purchased from AllCells LLC. (Emeryville, CA)Full Paper
2010Figueroa, ME;Lugthart, S;Li, Y;Erpelinck-Verschueren, C;Deng, X;Christos, PJ;Schifano, E;Booth, J;van Putten, W;Skrabanek, L;Campagne, F;Mazumdar, M;Greally, JM;Valk, PJ;Löwenberg, B;Delwel, R;Melnick, A;DNA methylation signatures identify biologically distinct subtypes in acute myeloid leukemiaCancer cell20060365We hypothesized that DNA methylation distributes into specific patterns in cancer cells, which reflect critical biological differences. We therefore examined the methylation profiles of 344 SaveFull Paper
2010Liu, W;Lee, HW;Liu, Y;Wang, R;Rodgers, GP;Olfactomedin 4 is a novel target gene of retinoic acids and 5-aza-2'-deoxycytidine involved in human myeloid leukemia cell growth, differentiation, and apoptosisBlood20724538Peripheral blood mononuclear cells from AML patients and normal individuals, and neutrophils from normal individuals, were obtained commercially from AllCells. The neutrophils were SaveFull Paper
2010Tatsumi, K;Taatjes, D;Bouchard, B;Trotman, W;Bovill, E;Cell Adhesion Molecule 1 (CADM1), a Novel Venous Thrombosis Risk Factor, Is Ubiquitously Present In Vascular Endothelium and Smooth Muscle CellsBloodImmunoelectron microscopy (IEM) was performed on cultured human umbilical vein endothelial cells (Allcells LLC, Emeryville, CA) using a post embedding procedure with protein-A SaveFull Paper
2010Lin, X;Rice, K;Carroll, M;Costa, F;Kilpivaara, O;Soares, M;Levine, R;Licht, J;Mir-9 Is Aberrantly Expressed In MPN Patients and Accelerates Erythropoietic Cell Growth and DifferentiationBloodTo determine how deregulated expression of miR-9 affected erythropoiesis, we transduced human bone marrow CD34+ cells (AllCells) with empty vector or miR-9 lentivirus, and Save;Abstract 1983 The myeloproliferative neoplasms (MPNs), PFull Paper
2010Campbell, JE;Brummel-Ziedins, KE;Butenas, S;Mann, KG;Cellular regulation of blood coagulation: a model for venous stasisBlood20864579We have adapted the corn-trypsin inhibitor whole-blood model to include EA.hy926 as an endothelium surrogate to evaluate the vascular modulation of blood coagulation initiated by SaveFull Paper
2010Sangokoya, C;Telen, MJ;Chi, JT;microRNA miR-144 modulates oxidative stress tolerance and associates with anemia severity in sickle cell diseaseBlood20709907Normal hemoglobin (HbAA) CD34+ cells (ALLCells) and HbSS CD34+ cells were purified from blood collected from apheresis with donor consent according to Duke Institutional Review Board protocol. Mononuclear cells were separated by Ficoll-Hypaque densitFull Paper
2010Prabhala, RH;Pelluru, D;Fulciniti, M;Prabhala, HK;Nanjappa, P;Song, W;Pai, C;Amin, S;Tai, YT;Richardson, PG;Ghobrial, IM;Treon, SP;Daley, JF;Anderson, KC;Kutok, JL;Munshi, NC;Elevated IL-17 produced by TH17 cells promotes myeloma cell growth and inhibits immune function in multiple myelomaBlood20395418approval by the institutional review board (IRB) at Dana-Farber Cancer Institute. Healthy donor samples were obtained from the blood donor center at Children's Hospital, Boston, MA. Normal donor bone marrow samples were obtained from AllCellsFull Paper
2010Liu, X;Shin, N;Koblish, HK;Yang, G;Wang, Q;Wang, K;Leffet, L;Hansbury, MJ;Thomas, B;Rupar, M;Waeltz, P;Bowman, KJ;Polam, P;Sparks, RB;Yue, EW;Li, Y;Wynn, R;Fridman, JS;Burn, TC;Combs, AP;Newton, RC;Scherle, PA;Selective inhibition of IDO1 effectively regulates mediators of antitumor immunityBlood20197554Bone marrow mononuclear cells of patients with AML were purchased from ALLCELLS and maintained according to the recommended culture conditions. SaveFull Paper
2010Quintás-Cardama, A;Vaddi, K;Liu, P;Manshouri, T;Li, J;Scherle, PA;Caulder, E;Wen, X;Li, Y;Waeltz, P;Rupar, M;Burn, T;Lo, Y;Kelley, J;Covington, M;Shepard, S;Rodgers, JD;Haley, P;Kantarjian, H;Fridman, JS;Verstovsek, S;Preclinical characterization of the selective JAK1/2 inhibitor INCB018424: therapeutic implications for the treatment of myeloproliferative neoplasmsBlood20130243Mononuclear cells were isolated from peripheral blood from patients with PV or normal control persons by centrifugation through Ficoll (Allcells). A total of 2 × 10 5 cells from control or SaveFull Paper
2010Swift, S;Ellison, AR;Kassner, P;McCaffery, I;Rossi, J;Sinclair, AM;Begley, CG;Elliott, S;Absence of functional EpoR expression in human tumor cell linesBlood20124514Erythroid progenitor cells were generated from CD34 + peripheral blood cells mobilized with granulocyte colony-stimulating factor (G-CSF; ALLCELLS) cultured for 6 days in StemSpan SaveFull Paper
2010Driscoll, JJ;Pelluru, D;Lefkimmiatis, K;Fulciniti, M;Prabhala, RH;Greipp, PR;Barlogie, B;Tai, YT;Anderson, KC;Shaughnessy, JD;Annunziata, CM;Munshi, NC;The sumoylation pathway is dysregulated in multiple myeloma and is associated with adverse patient outcomeBlood19965618Fresh, normal, human adult bone marrow was from AllCells, and CD138 + cells were affinity purified by positive selection using Miltenyi CD138 + microbeads (Miltenyi Biotec) according SaveFull Paper
2010Lee, CH;Shah, B;Moioli, EK;Mao, JJ;CTGF directs fibroblast differentiation from human mesenchymal stem/stromal cells and defines connective tissue healing in a rodent injury modelThe Journal of clinical investigation20679726Human MSCs were isolated from fresh whole bone marrow samples of 2 anonymous adult donors (age range, 20-25 years; AllCells) and were exempt from IRB approval. Nonhematopoietic, mononucleated and adherent cells were purified by centrifugation throughFull Paper
2010Chao, MP;Jaiswal, S;Weissman-Tsukamoto, R;Alizadeh, AA;Gentles, AJ;Volkmer, J;Weiskopf, K;Willingham, SB;Raveh, T;Park, CY;Majeti, R;Weissman, IL;Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47Science translational medicine21178137Normal human bone marrow mononuclear cells were purchased from AllCells Inc. Normal peripheral blood and human cancer samples were obtained from patients at the Stanford SaveFull Paper
2010Collins, JM;Ayala, P;Desai, TA;Russell, B;Three-dimensional culture with stiff microstructures increases proliferation and slows osteogenic differentiation of human mesenchymal stem cellsSmall (Weinheim an der Bergstrasse, Germany)19943257however, experiments were performed on P2 hMSCs obtained from 2 separate donors. Additionally, some experiments were repeated with P2 hMSCs isolated from whole marrow (AllCells, Berkely, CA) as described previously.[29]Full Paper
2010Cheng, S;Gao, N;Zhang, Z;Chen, G;Budhraja, A;Ke, Z;Son, YO;Wang, X;Luo, J;Shi, X;Quercetin induces tumor-selective apoptosis through downregulation of Mcl-1 and activation of BaxClinical cancer research : an official journal of the American Association for Cancer Research21138867Mononuclear cells were isolated from peripheral blood or bone marrow of leukemia patients or healthy donors were purchased from AllCells, LLC. Mononuclear cells were suspended SaveFull Paper
2010Berkofsky-Fessler, W;Buzzai, M;Kim, MK;Fruchtman, S;Najfeld, V;Min, DJ;Costa, FF;Bischof, JM;Soares, MB;McConnell, MJ;Zhang, W;Levine, R;Gilliland, DG;Calogero, R;Licht, JD;Transcriptional profiling of polycythemia vera identifies gene expression patterns both dependent and independent from the action of JAK2V617FClinical cancer research : an official journal of the American Association for Cancer Research20601445Normal bone marrow CD34 + cells were purchased from Allcells. To isolate CD34 + cells, bone marrow samples were diluted 1:1 in PBS and layered onto Histopaque 1077 (Sigma; SaveFull Paper
2010Marchi, E;Paoluzzi, L;Scotto, L;Seshan, VE;Zain, JM;Zinzani, PL;O'Connor, OA;Pralatrexate is synergistic with the proteasome inhibitor bortezomib in in vitro and in vivo models of T-cell lymphoid malignanciesClinical cancer research : an official journal of the American Association for Cancer Research20501616Peripheral blood mononuclear cells (PBMC) from healthy donors were purchased from Allcells. All cell lines were grown as previously described (21). SaveFull Paper
2010Paoluzzi, L;Scotto, L;Marchi, E;Zain, J;Seshan, VE;O'Connor, OA;Romidepsin and belinostat synergize the antineoplastic effect of bortezomib in mantle cell lymphomaClinical cancer research : an official journal of the American Association for Cancer Research20068080Mononuclear cells from PBMC samples of healthy donors were purchased from Allcells. All cell lines were grown as described previously (12, 13). SaveFull Paper
2010Brunckhorst, MK;Wang, H;Lu, R;Yu, Q;Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesisCancer research20823154Human umbilical vein ECs (HUVEC) and normal human astrocytes (NHA) were from AllCells, Inc., U87MG was obtained from American Type Culture Collection, and U251 was SaveFull Paper
2010Cho, SY;Xu, M;Roboz, J;Lu, M;Mascarenhas, J;Hoffman, R;The effect of CXCL12 processing on CD34+ cell migration in myeloproliferative neoplasmsCancer research20388788Fresh normal marrow was purchased from AllCells LLC. Cord blood (CB) collections were provided by Cord Blood Bank of New York Blood Center. SaveFull Paper
2010Xu, Y;Stamenkovic, I;Yu, Q;CD44 attenuates activation of the hippo signaling pathway and is a prime therapeutic target for glioblastomaCancer research20197461C, expression of endogenous CD44 in a panel of human glioma cells and NHAs (ALLCELLS, Inc.) was determined by Western blotting using anti-CD44 antibody (Santa Cruz). Total SaveFull Paper
2010Hart, S;Goh, K;Tan, Y;Amalini, C;Wood, J;Abstract 2524: SB1518, a novel oral JAK2-FLT3 inhibitor, is efficacious in models of acute myeloid leukemia and primary leukemic cells from patientsCancer ResearchFor progenitor expansion, PBMCs of AML patients (AllCells Inc) were cultured using a medium containing IL-3, IL-6, SCF and FLT3L (StemCell Technologies TB 28440). The expanded SaveFull Paper
2010Santanam, U;Zanesi, N;Efanov, A;Costinean, S;Palamarchuk, A;Hagan, JP;Volinia, S;Alder, H;Rassenti, L;Kipps, T;Croce, CM;Pekarsky, Y;Chronic lymphocytic leukemia modeled in mouse by targeted miR-29 expressionProceedings of the National Academy of Sciences of the United States of America20566844Real-time PCR experiments were carried out using miR-29a, miR-29b, and PXDN assays for real-time PCR (Applied Biosystems) according to the manufacturer's protocol. Control human cord blood CD19+ B cells were purchased from Allcells and LonzaFull Paper
2010Mi, S;Li, Z;Chen, P;He, C;Cao, D;Elkahloun, A;Lu, J;Pelloso, LA;Wunderlich, M;Huang, H;Luo, RT;Sun, M;He, M;Neilly, MB;Zeleznik-Le, NJ;Thirman, MJ;Mulloy, JC;Liu, PP;Rowley, JD;Chen, J;Aberrant overexpression and function of the miR-17-92 cluster in MLL-rearranged acute leukemiaProceedings of the National Academy of Sciences of the United States of America20133587obtained at the time of diagnosis or relapse and with informed consent at the University of Chicago or other hospitals (see characteristics of patients in Table S2). Normal control cells were purchased from AllCells. (Emeryville, CA)Full Paper
2010Wu, PK;Ringeisen, BR;Development of human umbilical vein endothelial cell (HUVEC) and human umbilical vein smooth muscle cell (HUVSMC) branch/stem structures on hydrogel layers via biological laser printing (BioLP)Biofabrication20811126HUVEC were purchased from Allcells, and HUVSMC were purchased from ATCC. Matrigel was purchased from BD Bioscience. The cells were grown in a humidified environment with 5 SaveFull Paper
2010Bhatwadekar, AD;Guerin, EP;Jarajapu, YP;Caballero, S;Sheridan, C;Kent, D;Kennedy, L;Lansang, MC;Ruscetti, FW;Pepine, CJ;Higgins, PJ;Bartelmez, SH;Grant, MB;Transient inhibition of transforming growth factor-beta1 in human diabetic CD34+ cells enhances vascular reparative functionsDiabetes20460428CD34 + cells enriched by Miltenyi positive selection from human cord blood and bone marrow obtained from AllCells (Emeryville, CA) and were >95% pure as assessed by fluorescence SaveFull Paper
2010Lin, TH;Hegen, M;Quadros, E;Nickerson-Nutter, CL;Appell, KC;Cole, AG;Shao, Y;Tam, S;Ohlmeyer, M;Wang, B;Goodwin, DG;Kimble, EF;Quintero, J;Gao, M;Symanowicz, P;Wrocklage, C;Lussier, J;Schelling, SH;Hewet, AG;Xuan, D;Krykbaev, R;Togias, J;Xu, X;Harrison, R;Mansour, T;Collins, M;Clark, JD;Webb, ML;Seidl, KJ;Selective functional inhibition of JAK-3 is sufficient for efficacy in collagen-induced arthritis in miceArthritis and rheumatism20506481Cryopreserved peripheral blood mononuclear cells (PBMCs; AllCells) were cultured in complete RPMI 1640 medium (supplemented with 10% fetal bovine serum, penicillin/streptomycin, 2 mM L-glutamine, and 1 mM sodium pyruvate) plus 10 μg/ml lectin phytoheFull Paper
2010Kodippili, GC;Spector, J;Kang, GE;Liu, H;Wickrema, A;Ritchie, K;Low, PS;Analysis of the kinetics of band 3 diffusion in human erythroblasts during assembly of the erythrocyte membrane skeletonBritish journal of haematology20553270Baxter Healthcare, Inc., Deerfield, IL) from commercially available growth factor mobilized blood samples (AllCells LLC, Emeryville, CA), as described elsewhere (Kang, et al 2008). Briefly, CD34+ cells isolated from growth-factor mobilized peripheralFull Paper
2010Lavelle, D;Saunthararajah, Y;Vaitkus, K;Singh, M;Banzon, V;Phiasivongsva, P;Redkar, S;Kanekal, S;Bearss, D;Shi, C;Inloes, R;DeSimone, J;S110, a novel decitabine dinucleotide, increases fetal hemoglobin levels in baboons (P. anubis)Journal of translational medicine20932295Frozen CD34+ human cells purified from the peripheral blood of mobilized donors were purchased from Allcells, Inc. These cells were cultured in Iscove's media containing 20% fetal SaveFull Paper
2010Yamaza, T;Kentaro, A;Chen, C;Liu, Y;Shi, Y;Gronthos, S;Wang, S;Shi, S;Immunomodulatory properties of stem cells from human exfoliated deciduous teethStem cell research & therapy20504286Healthy bone marrow aspirates from iliac bone and peripheral blood mononuclear cells (PBMNCs) of healthy volunteers were purchased from AllCells (Berkeley, CA, USA). SaveFull Paper
2010Luo, XM;Lei, MY;Feidi, RA;West, AP;Balazs, AB;Bjorkman, PJ;Yang, L;Baltimore, D;Dimeric 2G12 as a potent protection against HIV-1PLoS pathogens21187894ting amounts of monomer or dimer from the separated purified species. Humanized mice, passive transfer, and HIV-1 challenge Frozen human cord blood CD34+ cells from single donors were purchased from AllCells (Emeryville, CA) or Lonza (Basel, SwitzerFull Paper
2010Ellis-Connell, AL;Iempridee, T;Xu, I;Mertz, JE;Cellular microRNAs 200b and 429 regulate the Epstein-Barr virus switch between latency and lytic replicationJournal of virology20668090Raji cell assay as described above. Approximately 1 × 105 GRUs/ml was used to infect 1 × 106 cells/ml of primary blood B cells (AllCells, Emeryville, CA). Samples were harvested at the times indicated in Fig. 2 and processed as described above for ZEFull Paper
2010Li, J;Favata, M;Kelley, JA;Caulder, E;Thomas, B;Wen, X;Sparks, RB;Arvanitis, A;Rogers, JD;Combs, AP;Vaddi, K;Solomon, KA;Scherle, PA;Newton, R;Fridman, JS;INCB16562, a JAK1/2 selective inhibitor, is efficacious against multiple myeloma cells and reverses the protective effects of cytokine and stromal cell supportNeoplasia (New York, N.Y.)20072651Primary bone marrow CD138+ plasma cells from a newly diagnosed MM patient were purchased from Allcells (Emeryville, CA). The cells were cultured in the same medium used for SaveFull Paper
2010Jekle, A;Chhabra, M;Lochner, A;Meier, S;Chow, E;Brandt, M;Sankuratri, S;Cammack, N;Heilek, G;Epitope switching as a novel escape mechanism of HIV to CCR5 monoclonal antibodiesAntimicrobial agents and chemotherapy1999592310% FBS and 1% penicillin-streptomycin. Human peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA), stimulated for 1 day in PBMC medium (RPMI 1640 medium containing 10% FBS, 1% penicillin-streptomycin, 2 mM l-glutamiFull Paper
2010Culpin, RE;Proctor, SJ;Angus, B;Crosier, S;Anderson, JJ;Mainou-Fowler, T;A 9 series microRNA signature differentiates between germinal centre and activated B-cell-like diffuse large B-cell lymphoma cell linesInternational journal of oncology20596664streptomycin (Gibco), according to the repositories guidelines. Cultures were incubated at 37˚C in a 5% CO2 humidified atmosphere. CD19+ negatively-selected B-cell total RNA (pooled from 3 unrelated donors) served as the normal healthy B-cell controlFull Paper
2010Collins, JM;Goldspink, PH;Russell, B;Migration and proliferation of human mesenchymal stem cells is stimulated by different regions of the mechano-growth factor prohormoneJournal of molecular and cellular cardiology208758252 mM L-glutamine, 100 units/mL penicillin and 100 μg/mL streptomycin. hMSCs were obtained from the Tulane University Center for Gene Therapy or AllCells (Berkeley, CA). Passage 2-3 (P2-P3) hMSCs were used for all experimentsFull Paper
2010Destache, CJ;Belgum, T;Goede, M;Shibata, A;Belshan, MA;Antiretroviral release from poly(DL-lactide-co-glycolide) nanoparticles in miceThe Journal of antimicrobial chemotherapy20729545Frozen elutriated monocytes were obtained from AllCells, LLC (Emeryville, CA, USA). Cells were thawed in a water bath at 37C and counted using Trypan Blue exclusion. Cells (5 × 10 SaveFull Paper
2010Berahovich, RD;Zabel, BA;Penfold, ME;Lewén, S;Wang, Y;Miao, Z;Gan, L;Pereda, J;Dias, J;Slukvin, II;McGrath, KE;Jaen, JC;Schall, TJ;CXCR7 protein is not expressed on human or mouse leukocytesJournal of immunology (Baltimore, Md. : 1950)20889540Human normal bone marrow cells were purchased from AllCells (Emeryville, CA). Human thymocytes were obtained by mechanical disruption of fetal thymus tissue purchased from SaveFull Paper
2010Thacker, SG;Berthier, CC;Mattinzoli, D;Rastaldi, MP;Kretzler, M;Kaplan, MJ;The detrimental effects of IFN-α on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefactionJournal of immunology (Baltimore, Md. : 1950)20805419Similar experiments were performed with EPCs directly obtained from control bone marrow. In brief, CD133+ cells obtained from healthy control bone marrows (AllCells, Emeryville, CA) were thawed following manufacturer’s recommendations and then culturFull Paper
2010Toti, US;Guru, BR;Grill, AE;Panyam, J;Interfacial activity assisted surface functionalization: a novel approach to incorporate maleimide functional groups and cRGD peptide on polymeric nanoparticles for targeted drug deliveryMolecular pharmaceutics20527782methyl tri-chlorosilane were from Sigma-Aldrich (St Louis, MO). RPMI cell media, fetal bovine serum, and trypsin were purchased from Invitrogen (Carlsbad, CA). HUVEC media was supplied by AllCells (Emeryville, CA)Full Paper
2010Medina, RJ;O'Neill, CL;Humphreys, MW;Gardiner, TA;Stitt, AW;Outgrowth endothelial cells: characterization and their potential for reversing ischemic retinopathyInvestigative ophthalmology & visual science20554606of 1 × 107 cells/mL. Population doubling was carefully monitored, and OECs at early passages were used for all experiments. Frozen human cord blood mononuclear cells were purchased (Allcells; Stem Cell Technologies, Emeryville, CA) and plated under tFull Paper
2010Kopher, RA;Penchev, VR;Islam, MS;Hill, KL;Khosla, S;Kaufman, DS;Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cellsBone20601304Mayo Institutional Review Board and obtaining informed consent, or isolated from whole bone marrow (AllCells, Emeryville, CA, USA) using standard methods [50]. BM-MSCs were cultured in MSC media as described above. Adherent cells were selected for duFull Paper
2010Vanella, L;Kim, DH;Asprinio, D;Peterson, SJ;Barbagallo, I;Vanella, A;Goldstein, D;Ikehara, S;Kappas, A;Abraham, NG;HO-1 expression increases mesenchymal stem cell-derived osteoblasts but decreases adipocyte lineageBone19853072Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential SaveFull Paper
2010Kim, DH;Vanella, L;Inoue, K;Burgess, A;Gotlinger, K;Manthati, VL;Koduru, SR;Zeldin, DC;Falck, JR;Schwartzman, ML;Abraham, NG;Epoxyeicosatrienoic acid agonist regulates human mesenchymal stem cell-derived adipocytes through activation of HO-1-pAKT signaling and a decrease in PPARγStem cells and development20412023Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α SaveFull Paper
2010Fox, LE;Shen, J;Ma, K;Liu, Q;Shi, G;Pappas, GD;Qu, T;Cheng, J;Membrane properties of neuron-like cells generated from adult human bone-marrow-derived mesenchymal stem cellsStem cells and development20394468The hMeSCs from 4 donors were commercially obtained from AllCells LLC and Cambrex Bioscience. The cell lines were negative for surface markers associated with hematopoietic SaveFull Paper
2010Lai, Y;Sun, Y;Skinner, CM;Son, EL;Lu, Z;Tuan, RS;Jilka, RL;Ling, J;Chen, XD;Reconstitution of marrow-derived extracellular matrix ex vivo: a robust culture system for expanding large-scale highly functional human mesenchymal stem cellsStem cells and development19737070Freshly isolated human bone marrow mononuclear cells obtained from 20- to 30-year-old donors were purchased from ALLCELLS (Emeryville, CA), and grown on tissue culture plastic SaveFull Paper
2010McLaughlin, J;Markovtsov, V;Li, H;Wong, S;Gelman, M;Zhu, Y;Franci, C;Lang, D;Pali, E;Lasaga, J;Low, C;Zhao, F;Chang, B;Gururaja, TL;Xu, W;Baluom, M;Sweeny, D;Carroll, D;Sran, A;Thota, S;Parmer, M;Romane, A;Clemens, G;Grossbard, E;Qu, K;Jenkins, Y;Kinoshita, T;Taylor, V;Holland, SJ;Argade, A;Singh, R;Pine, P;Payan, DG;Hitoshi, Y;Preclinical characterization of Aurora kinase inhibitor R763/AS703569 identified through an image-based phenotypic screenJournal of cancer research and clinical oncology19609559Cultured human primary mast cells were established from human umbilical cord blood CD34 + progenitor cells (AllCells, LLC, Berkeley, CA) using the method described in US patent no SaveFull Paper
2010Chen, NK;Tan, SY;Udolph, G;Kon, OL;Insulin expressed from endogenously active glucose-responsive EGR1 promoter in bone marrow mesenchymal stromal cells as diabetes therapyGene therapy20182520HBMMSC (AllCells, Emeryville, CA, USA) cultured overnight in Dulbecco's modified Eagle's medium (DMEM)-3 mM glucose (as the low glucose baseline) were exposed to DMEM-20 mM glucose (high glucose) for 15 or 30 min, and then returned to DMEM-3 mM glucoFull Paper
2010Marchi, E;Pralatrexate sinergizza in vitro ed in vivo con l'inibitore del Proteosoma Bortezomib in modelli pre-clinici di Linfoma Non Hodkin TThesisaggiunto a giorni alterni. Le cellule sono state mantenute in coltura alla concentrazione ideale di 0.5 x 106 cellule/mL e trattate come descritto in precedenti studi pubblicati52 . Le cellule mononucleate del sangue periferico da donatori volontariFull Paper
2010Qureshi, A;Subathra, M;Grey, A;Schey, K;Del Poeta, M;Luberto, C;Role of sphingomyelin synthase in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformansPloS one21203393Approximately 6.4×10 4 fresh human normal peripheral blood-neutrophils per ml (AllCells) were incubated in 124 µl sterile filtered PBS supplemented with 10% pooled human serum SaveFull Paper
2010O'Connell, RM;Balazs, AB;Rao, DS;Kivork, C;Yang, L;Baltimore, D;Lentiviral vector delivery of human interleukin-7 (hIL-7) to human immune system (HIS) mice expands T lymphocyte populationsPloS one207004542×10 7 PBMCs (AllCells) that were first labeled with CFSE. HIS mice were created as described[5]. In brief, 1.5×10 5 highly purified CD34+ CB stem cells (AllCells) were injected into the SaveFull Paper
2010Smith, B;Treadwell, J;Zhang, D;Ly, D;McKinnell, I;Walker, PR;Sikorska, M;Large-scale expression analysis reveals distinct microRNA profiles at different stages of human neurodevelopmentPloS one20559549obtained from ScienCell Research Laboratories (Carlsbad, CA) and were directly pelleted for RNA extraction. Frozen primary human fetal astrocytes (PHAf) from 20-21 week fetuses were received from ALLCELLS (Emeryville, CA) and were cultured in AstrocFull Paper
2010Kachlany, SC;Schwartz, AB;Balashova, NV;Hioe, CE;Tuen, M;Le, A;Kaur, M;Mei, Y;Rao, J;Anti-leukemia activity of a bacterial toxin with natural specificity for LFA-1 on white blood cellsLeukemia research19747730The oral bacterium, Aggregatibacter actinomycetemcomitans, produces a leukotoxin (LtxA) that is specific for white blood cells (WBCs) from humans and Old World primates by SaveFull Paper
2010Medina, RJ;O'Neill, CL;Sweeney, M;Guduric-Fuchs, J;Gardiner, TA;Simpson, DA;Stitt, AW;Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identitiesBMC medical genomics20465783l approval from three female volunteer subjects aged 25-35, non-smokers, not receiving any medication and without any clinical diagnosis; while CB-derived mononuclear cells (MNCs) were purchased from AllCells LLC (California, USA). MNCs were isolatedFull Paper
2010Rowe, DD;Leonardo, CC;Hall, AA;Shahaduzzaman, MD;Collier, LA;Willing, AE;Pennypacker, KR;Cord blood administration induces oligodendrocyte survival through alterations in gene expressionBrain research20883670Oligodendrocytes (OLs), the predominant cell type found in cerebral white matter, are essential for structural integrity and proper neural signaling. Very little is known concerning stroke- SaveFull Paper
2010Jin, Q;Alkhatib, B;Cornetta, K;Alkhatib, G;Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1Virology19913864streptomycin sulfate (0.1 mg/ml) in a humidified incubator held at 5% CO2. All cell culture media were purchased from GIBCO. Normal Human Astrocytes (NHA) and their basal medium (with 10% FBS) were purchased from AllCells (AllCells, LLC., Emeryville,Full Paper
2010Walczak-Drzewiecka, A;Ratajewski, M;Pułaski, Ł;Dastych, J;DNA methylation-dependent suppression of HIF1A in an immature hematopoietic cell line HMC-1Biochemical and biophysical research communications19995553cDNAs from BM CD34+, PB CD34+, BM MNCs, PB MNCs and BM MSCs purchased from AllCells (Emeryville, CA, USA) was obtained from a population of positive cells (purity exceeding 95%) collected from five individual donors. The level of cognate cDNA was meaFull Paper
2010Li, W;Hong, L;Hu, L;Magin, RL;Magnetization transfer imaging provides a quantitative measure of chondrogenic differentiation and tissue developmentTissue engineering. Part C, Methods20373975Mesenchymal stem cells (MSCs), isolated from fresh adult human bone marrow obtained from AllCells LLC (Berkeley, CA), were plated at a density of 107 nucleated cells per 100-mm-diameter Petri dish. The cells were incubated in a basic medium of DulbecFull Paper
2010Akers, SM;O'Leary, HA;Minnear, FL;Craig, MD;Vos, JA;Coad, JE;Gibson, LF;VE-cadherin and PECAM-1 enhance ALL migration across brain microvascular endothelial cell monolayersExperimental hematology20470859Positively selected peripheral blood CD19 + B cells from a healthy donor were purchased from AllCells (Emeryville, CA, USA). HBMEC were obtained from Angioproteomie (Boston, MA SaveFull Paper
2010Schuhmann, NK;Pozzoli, O;Sallach, J;Huber, A;Avitabile, D;Perabo, L;Rappl, G;Capogrossi, MC;Hallek, M;Pesce, M;Büning, H;Gene transfer into human cord blood-derived CD34(+) cells by adeno-associated viral vectorsExperimental hematology20447441After thawing, hCD34 + cells (AllCells) were plated and expanded for 3 days as described. Cells were then incubated with rAAV2sc (at a GOI of 1 × 10 4 ) for 18 hours, followed by an SaveFull Paper
2010Lu, M;Zhang, W;Li, Y;Berenzon, D;Wang, X;Wang, J;Mascarenhas, J;Xu, M;Hoffman, R;Interferon-alpha targets JAK2V617F-positive hematopoietic progenitor cells and acts through the p38 MAPK pathwayExperimental hematology20303384Objective Interferon-α (IFNα) therapy leads to hematological remissions and a reduction of the JAK2V617F allele burden in patients with polycythemia vera (PV). In this study, the SaveFull Paper
2010Yourek, G;McCormick, SM;Mao, JJ;Reilly, GC;Shear stress induces osteogenic differentiation of human mesenchymal stem cellsRegenerative medicine20868327Isolated bone marrow (AllCells, Berkeley, CA, USA) from multiple healthy human donors aged between 20 and 26 years (seven male and one female) was enriched for hMSCs using RosetteSep® (StemCell Technologies, Vancouver, BC, Canada). Bone marrow was inFull Paper
2010Barbagallo, I;Vanella, A;Peterson, SJ;Kim, DH;Tibullo, D;Giallongo, C;Vanella, L;Parrinello, N;Palumbo, GA;Di Raimondo, F;Abraham, NG;Asprinio, D;Overexpression of heme oxygenase-1 increases human osteoblast stem cell differentiationJournal of bone and mineral metabolism19924377Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA, USA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential SaveFull Paper
2010Covey, TM;Putta, S;Cesano, A;Single cell network profiling (SCNP): mapping drug and target interactionsAssay and drug development technologies20158439Cryopreserved, Ficoll fractionated, bone marrow samples from healthy individual were purchased from AllCells (Emeryville, CA). Cells from cryogenic storage were thawed in a 37C SaveFull Paper
2010Bingaman, MG;Basu, GD;Golding, TC;Chong, SK;Lassen, AJ;Kindt, TJ;Lipinski, CA;The autophilic anti-CD20 antibody DXL625 displays enhanced potency due to lipid raft-dependent induction of apoptosisAnti-cancer drugs20216307blood lymphocytes (PBLs) were purified from healthy donor samples using standard Ficoll-Plaque separation. Normal human peripheral blood mononuclear cells (PBMCs) were from AllCells (Emeryville, California, USA). CD56-immunomagnetic separation beadsFull Paper
2010Elliott, S;Busse, L;McCaffery, I;Rossi, J;Sinclair, A;Spahr, C;Swift, S;Begley, CG;Identification of a sensitive anti-erythropoietin receptor monoclonal antibody allows detection of low levels of EpoR in cellsJournal of immunological methods19887071Human EpoR expressing erythroid primary cells were derived from human mobilized peripheral blood CD34+ cells (AllCells, LLC, Emeryville, CA) by culture for 6 days in StemSpan SFEM media (STEMCELLS Tech; Vancouver, BC, Canada) with 10 ng/ml IL-3, 10 nFull Paper
2010Kim, ES;Kim, JJ;Park, EJ;Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft materialThe journal of advanced prosthodontics21165181Commercially available human apheresed platelet plasmas were purchased from AllCells (Emeryville, CA, USA). Donors were restricted to males under 30 years-old for quality control. SaveFull Paper
2010Shytle, DR;Tan, J;Ehrhart, J;Smith, AJ;Sanberg, CD;Sanberg, PR;Anderson, J;Bickford, PC;Effects of blue-green algae extracts on the proliferation of human adult stem cells in vitro: a preliminary studyMedical science monitor : international medical journal of experimental and clinical research20037479For cell proliferation analysis, human bone marrow cells or human CD34+ cells (AllCells, Inc.) were cultured in 96 well plates (5×10 4 /well) containing 100 µL of complete medium (RPMI 1640 medium supplemented with 5% FCS). These cells were treated fFull Paper
2010Kawauchi, S;Terasaki, H;Katano, M;Murase, J;Masuda, Y;Tamura, T;Shimadzu, M;Quality control and monitoring for the isolation process of mesenchymal stem cells and their differentiation into osteoblastsGenetic testing and molecular biomarkers20187761Bone marrow samples (ABM001; AllCells, Emeryville, CA) were purchased after gaining approval of the ethical 270committee of the Olympus Corporation. Table 1 shows the ages and sexes of the donors. Skin-derived fibroblasts were also purchased from LonFull Paper
2010Abbott, C;Huang, G;Ellison, AR;Chen, C;Arora, T;Szilvassy, SJ;Wei, P;Mouse monoclonal antibodies against human c-Mpl and characterization for flow cytometry applicationsHybridoma (2005)20443702Primary human CD34 + cells isolated from normal bone marrow or G-CSF-mobilized peripheral blood were purchased from AllCells (Emeryville, CA). Cryopreserved cells were thawed SaveFull Paper
2010Clarkson-Jones, J;Page, C;Sarda, S;Swaisland, H;417 Human biotransformation of olaparib (AZD2281) an oral poly(ADP-ribose) polymerase (PARP) inhibitorEuropean Journal of Cancer SupplementsMethods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommended by the manufacturer (StemCell Technologies). Effects of SBFull Paper
2010Nokihara, H;Yamamoto, N;Yamada, Y;Asahina, H;Shibata, T;Seki, Y;Tamura, Y;Honda, K;Misawa, S;Tamura, T;415 A phase I study evaluating the safety profile and pharmacokinetics of CS-1008 (Tigatuzumab), humanized monoclonal antibody targeting death receptor 5 (DR5), in Japanese patients with advanced solid tumoursEuropean Journal of Cancer SupplementsMethods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommended by the manufacturer (StemCell Technologies). Effects of SBFull Paper
2010Padgett, C;Lim, J;Marschke, R;Northfelt, D;Andreopoulou, E;Hoff, D;Anderes, K;Ryckman, D;Chen, T;O'Brien, S;414 Clinical pharmacokinetics and pharmacodynamics of CX-4945, a novel inhibitor of protein kinase CK2: Interim report from the phase 1 clinical trialEuropean Journal of Cancer Supplementsthat is currently in phase Ib clinical trials in AML and MDS. Methods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommenFull Paper
2010Antognoli, A;Rag2-/-; gammac-/-immunodeficient mice, a new preclinical model to study antitumor approachesThesisCD133+ human cord blood progenitor cells (derived from single donors) purchased from ALLCELLS (Emerville, CA), or kindly provided by Professor Lemoli of the Department of Hematology and Oncologic Sciences “L. e A. Seragnoli”, University of BolognaFull Paper
2010Tanavde, V;Commercialization of Cord BloodUmbilical Cord BloodAllCells, a California-based company and Lonza are leading players in this area. BioE also sells its MLPC cell line for research and is developing the use of MLPC as a platform for SaveFull Paper
2010Nowakiewicz, M;MST-based method for 6DOF rigid body motion planning in narrow passages2010 IEEE/RSJ International Conference on Intelligent Robots and Systemsconstruction of MST give priority to cells at levels equal or less than { by always treating their weights as lesser than weights of the other cells. 3) ALLCELLS() - Return the set of all leaf cells. 4) NEIGHBORHOOD(z) - Return the set of all cells iFull Paper
2010Briercheck, E;Freud, A;Caligiuri, M;Human natural killer cell developmentNatural Killer CellsFresh human BM was donated or purchased from AllCells, LLC (Berkeley, CA) and received within 24 hours of harvest. PB leukopaks were obtained from the American Red Cross. BM SaveFull Paper
2010Ringeisen, B;Othon, C;Wu, X;Krizman, D;Darfler, M;Anders, J;Wu, P;Biological Laser Printing (BioLP) for High Resolution Cell DepositionCell and Organ PrintingHUVEC were purchased from Allcells, LLC (Emeryville, California) and cultured according to the protocol provided. HUSMC were purchased from ATCC and cultured according to the protocol provided by the supplier. In both cases, per ml of media is fortifFull Paper
2010Leskov, I;Modeling hematologic malignancies and their treatment in humanized miceThesisPurified CD133* human umbilical cord hematopoietic stem cells (HSCs) were purchased from AllCells or from the National Disease Research Interchange (NDRI). Alternatively, CD133* cells were purified from fresh umbilical cord blood purchased from NDRI,Full Paper
2010Chao, MP;Modulating Phagocytosis: Therapeutic Targeting of the Anti-Phagocytic Signal CD47 in Human MalignanciesThesisHuman Samples Normal human bone marrow mononuclear cells were purchased from AllCells Inc. (Emeryville, CA, USA). Human AML samples (Figure 1B) were obtained from patients at the Stanford Medical Center with informed consent, according to an IRB-apprFull Paper
2010Lee, CH;Connective tissue growth factor (CCN2/CTGF)-induced fibrogenesis: Biology, bioengineering and therapeuticsThesisHuman mesenchymal stem cells (hMSCs) were isolated from commercially available fresh bone marrow samples of anonymous adult donors (AllCells, Berkeley, CA). Purification of hMSCs was accomplished by centrifugation through a density gradient (Ficoll-PFull Paper
2010Collins, JM;Stem cells are affected by physical and chemical components of the microenvironmental nicheThesisxperiments were performed on P2 hMSCs obtained from 2 separate donors. Additionally, some experiments were repeated with P2 hMSCs isolated from whole marrow (AllCells, Berkely, CA) as described previously (Alhadlaq et al., 2005)Full Paper
2010Scaife, MD;Truncated Cell Surface Markers Fused with Mutant Human Tmpk: Versatile Cell Fate Control Safety Cassettes for Lentiviral Vector Mediated Correction of Fabry DiseaseThesisHuman CD34+ cells were purchased from AllCells and cultured in Stem Span SFEM expansion medium (Stem Cell Technologies) and supplemented with 10 ng/ml human interleukin-6 (IL-6), 100 ng/ml human Flt3 Ligand (Flt3-L), 100 ng/ml human thrombopoietin (TFull Paper
2010Van Craenenbroeck, E;Exercise in chronic heart failure focus on endothelial repairThesistypesmost likely have important contributions to the initiation, continuation and regulation ofvascular homeostasis In the following, the term EPC is used for general description of allcells SaveFull Paper
2010Jangam, SR;A PCR based HIV-1 test for point-of-care applicationThesisIt was observed that there was a saturation effect being observed at total WBC 500,000 cells. To further confirm this observation, leukocyte depleted blood was spiked with different amounts of PBMC (ALLCELLS, Emeryville, CA) in the range of 46,000-2Full Paper
2010Sofia, MJ;Bao, D;Chang, W;Du, J;Nagarathnam, D;Rachakonda, S;Reddy, PG;Ross, BS;Wang, P;Zhang, HR;Bansal, S;Espiritu, C;Keilman, M;Lam, AM;Steuer, HM;Niu, C;Otto, MJ;Furman, PA;Discovery of a β-d-2'-deoxy-2'-α-fluoro-2'-β-C-methyluridine nucleotide prodrug (PSI-7977) for the treatment of hepatitis C virusJournal of medicinal chemistry20845908We acknowledge the following individuals and organizations for their contributions to the work described in this manuscript: Wuxi AppTec; ReachBio LLC; BASi; and Dr. Patrick Carroll, University of PennsylvaniaFull Paper
2010Lam, AM;Murakami, E;Espiritu, C;Steuer, HM;Niu, C;Keilman, M;Bao, H;Zennou, V;Bourne, N;Julander, JG;Morrey, JD;Smee, DF;Frick, DN;Heck, JA;Wang, P;Nagarathnam, D;Ross, BS;Sofia, MJ;Otto, MJ;Furman, PA;PSI-7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype inhibitor of hepatitis C virus replicationAntimicrobial agents and chemotherapy20516278Human bone marrow progenitor cell toxicity assays were performed by Emer Clarke at ReachBio LLC (Seattle, WA)Full Paper
2010Maloney, JM;Nikova, D;Lautenschläger, F;Clarke, E;Langer, R;Guck, J;Van Vliet, KJ;Mesenchymal stem cell mechanics from the attached to the suspended stateBiophysical journal20959088artment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts ¶Department of Physics, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom ‖ReachBio, Seattle, Washington ∗Corresponding author kFull Paper
2009Nemunaitis, J;Clayman, G;Agarwala, SS;Hrushesky, W;Wells, JR;Moore, C;Hamm, J;Yoo, G;Baselga, J;Murphy, BA;Menander, KA;Licato, LL;Chada, S;Gibbons, RD;Olivier, M;Hainaut, P;Roth, JA;Sobol, RE;Goodwin, WJ;Biomarkers Predict p53 Gene Therapy Efficacy in Recurrent Squamous Cell Carcinoma of the Head and NeckClin. Cancer Res.19996201iplex primers (Affymetrix, Inc.). The resultant PCR products were analyzed using the Affymetrix p53 GeneChip, which encompasses all coding exons, 2 to 11, as well as immediate flanking regions. Confirmation of the results was carried out by sequencinFull Paper
2009Mori, F;Oda, N;Sakuragi, M;Sakakibara, F;Kiniwa, M;Miyoshi, K;New histopathological experimental model for benign prostatic hyperplasia: stromal hyperplasia in ratsJ. Urol.19095262All sections were developed by the avidin-biotin complex method. Sections were also stained with hematoxylin and eosin, and Masson's trichrome. Human BPH tissue samples (Folio Biosciences, Columbus, Ohio) were also stained. Histological AnalysisFull Paper
2009Fazzone, W;Wilson, PM;Labonte, MJ;Lenz, HJ;Ladner, RD;Histone deacetylase inhibitors suppress thymidylate synthase gene expression and synergize with the fluoropyrimidines in colon cancer cellsInt. J. Cancer19384949liquid nitrogen or fixed in 10% formalin. Formalin‐fixed tumor specimens were paraffin‐embedded and subsequently analyzed by immunohistochemistry (IHC) (QualTek Laboratories, Newtown, PA). IHC for TS was conducted asFull Paper
2009Donkor, J;Zhang, P;Wong, S;O'Loughlin, L;Dewald, J;Kok, BP;Brindley, DN;Reue, K;A conserved serine residue is required for the phosphatidate phosphatase activity but not the transcriptional coactivator functions of lipin-1 and lipin-2J. Biol. Chem.19717560Invitrogen). Plasmids were sequences to select templates for sense and antisense probe generation and linearized by digestion with BamHI. In situ hybridization was performed using the services of Phylogeny, Inc. (Columbus, OH)Full Paper
2009Maxwell, SA;Li, Z;Jaye, D;Jaya, D;Ballard, S;Ferrell, J;Fu, H;14-3-3zeta mediates resistance of diffuse large B cell lymphoma to an anthracycline-based chemotherapeutic regimenJ. Biol. Chem.195252243′. Preparation of DLBCL Tissue Extracts for Western Blotting. Normal and DLBCL lymph node tissues were procured through Folio Biosciences. Frozen tissue was ground to a powder in liquid nitrogen in a pestle and mortarFull Paper
2009Deroo, BJ;Rodriguez, KF;Couse, JF;Hamilton, KJ;Collins, JB;Grissom, SF;Korach, KS;Estrogen receptor beta is required for optimal cAMP production in mouse granulosa cellsMol. Endocrinol.19324971Mice were treated with vehicle or PMSG for 48 h, after which the ovaries were removed and snap frozen in 2-methylbutane (isopentane) and then stored at −70 C. In situ hybridization analysis for the detection of Lhcgr in whole ovaries was then carriedFull Paper
2009Cunningham, D;Spychala, K;McLarren, KW;Garza, LA;Boerkoel, CF;Herman, GE;Developmental expression pattern of the cholesterogenic enzyme NSDHL and negative selection of NSDHL-deficient cells in the heterozygous Bpa(1H)/+ mouseMol. Genet. Metab.19631568A darkfield view of a similar section hybridized with a control 35S-labeled sense Nsdhl RNA probe, showing little or no nonspecific background hybridization. (D) A brightfield view of a lateral sagittal section of the E15.5 embryo that includes the eFull Paper
2009Chen, LM;Verity, NJ;Chai, KX;Loss of prostasin (PRSS8) in human bladder transitional cell carcinoma cell lines is associated with epithelial-mesenchymal transition (EMT)BMC Cancer19849847(Hayward, CA). A Bladder Carcinoma TMA (ARY-HH0087) containing 80 tissue cores, each at 1.5 mm in diameter; with 40 cancer tissues and 40 matching or independent non-cancerous tissues was obtained from Folio Biosciences (Columbus, OH)Full Paper
2009Binkert, CA;Drooz, AT;Caridi, JG;Sands, MJ;Bjarnason, H;Lynch, FC;Rilling, WS;Zambuto, DA;Stavropoulos, SW;Venbrux, AC;Kaufman, JA;Technical success and safety of retrieval of the G2 filter in a prospective, multicenter studyJ Vasc Interv Radiol19875062Full Paper
2009Lynch, FC;Balloon-assisted removal of tilted inferior vena cava filters with embedded tipsJ Vasc Interv Radiol19729132Full Paper
2009Lynch, FC;Kekulawela, S;Removal of the G2 filter: differences between implantation times greater and less than 180 daysJ Vasc Interv Radiol19640738Full Paper
2009Cantwell, CP;Pennypacker, J;Singh, H;Scorza, LB;Waybill, PN;Lynch, FC;Comparison of the recovery and G2 filter as retrievable inferior vena cava filtersJ Vasc Interv Radiol19640733Full Paper
2009Choi, KD;Vodyanik, MA;Slukvin, II;Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitorsThe Journal of clinical investigation19726877CD1a+ DCs, or LCs were cocultured with 1 × 105 adult peripheral (AllCells) or cord blood (Cambrex) mononuclear cells in RPMI 1640 medium (Invitrogen) containing 5% human AB serum (Sigma-Aldrich) in 96-well flat-bottom plates. At 6 days of culture, thFull Paper
2009Pfeffer, BA;Bernstein, SA;Bartels, SP;Preservation of structure and immunoreactivity at the vitreoretinal interface of the rabbit eyeGraefes Arch. Clin. Exp. Ophthalmol.19020891Presented in part at the 2006 meeting of The Association for Research in Vision and Ophthalmology, Fort Lauderdale, Florida. This study was supported by Bausch & Lomb, Inc., with whom QualTek Molecular Laboratories has a contractual relationshipFull Paper
2009Petrie, KR;Schenk, T;Chornet, MB;Howell, L;Dick, JE;Modulation of Histone H3K4 and H3K27 Methylation Levels Via Pharmacological Inhibition of LSD1 and Degradation of the EZH2-Containing Polycomb Repressive Complex 2 Stimulates ATRA-Mediated Differentiation of AML CellsConferenceFlow cytometric detection of apoptosis among normal mononuclear cells obtained from the bone marrow of healthy volunteers (AllCells, LLC) by phycoerythrin (PE)conjugated Annexin- SaveFull Paper
2009Muchamuel, T;Basler, M;Aujay, MA;Suzuki, E;Kalim, KW;Lauer, C;Sylvain, C;Ring, ER;Shields, J;Jiang, J;Shwonek, P;Parlati, F;Demo, SD;Bennett, MK;Kirk, CJ;Groettrup, M;A selective inhibitor of the immunoproteasome subunit LMP7 blocks cytokine production and attenuates progression of experimental arthritisNature medicine19525961We purchased human PBMCs from normal healthy volunteers from AllCells, and we obtained PBMCs from individuals with rheumatoid arthritis (who had given informed consent) from a SaveFull Paper
2009Majeti, R;Chao, MP;Alizadeh, AA;Pang, WW;Jaiswal, S;Gibbs, KD;van Rooijen, N;Weissman, IL;CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cellsCell19632179Acute myeloid leukemia (AML) is organized as a cellular hierarchy initiated and maintained by a subset of self-renewing leukemia stem cells (LSC). We hypothesized that increased SaveFull Paper
2009Schmitt, N;Morita, R;Bourdery, L;Bentebibel, SE;Zurawski, SM;Banchereau, J;Ueno, H;Human dendritic cells induce the differentiation of interleukin-21-producing T follicular helper-like cells through interleukin-12Immunity19592276sorting with FACSAria (BD) as CCR7+ CD8- CD56- HLA-DR- CD45RA+ CD4+ cells. Memory CD4+ T cells were directly sorted from PBMCs as CD8- CD56- CD45RA- CD4+ cells. Cord blood naïve CD4 T cells were sorted from frozen cells (AllCells) as CCR7+ CD8- CD56-Full Paper
2009Wang, Y;Sul, HS;Pref-1 regulates mesenchymal cell commitment and differentiation through Sox9Cell metabolism19254573Human-derived marrow stromal cells (MSC) and MSC media were purchased from Allcells. Sox9 target-specific siRNA (sc-36534) (Santa Cruz) and the control siRNA (sc-36869) were SaveFull Paper
2009Trifari, S;Kaplan, CD;Tran, EH;Crellin, NK;Spits, H;Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cellsNature immunology19578368Naive CD4 + T cells were obtained from human cord blood (AllCells) with a Naive CD4 + T cell Isolation Kit II (Miltenyi), followed by sorting of the CD4 + CD45RA + CD45RO − SaveFull Paper
2009Ye, Z;Zhan, H;Mali, P;Dowey, S;Williams, DM;Jang, YY;Dang, CV;Spivak, JL;Moliterno, AR;Cheng, L;Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disordersBlood19797525Isolated PB CD34 + cells after G-CSF mobilization were purchased from AllCells and used as a normal control for analyzing gene expression. Four classic retroviral vectors pMXs-Oct4, SaveFull Paper
2009Parlati, F;Lee, SJ;Aujay, M;Suzuki, E;Levitsky, K;Lorens, JB;Micklem, DR;Ruurs, P;Sylvain, C;Lu, Y;Shenk, KD;Bennett, MK;Carfilzomib can induce tumor cell death through selective inhibition of the chymotrypsin-like activity of the proteasomeBlood19671918Rosen's laboratory at Northwestern University. CD138+ cells and peripheral blood mononuclear cells (PBMCs) were purchased from Allcells. The purity of CD138+ cells was greater than 90% as determined by the supplier. HS-Sultan-GFPu cell lines were genFull Paper
2009Figueroa, ME;Skrabanek, L;Li, Y;Jiemjit, A;Fandy, TE;Paietta, E;Fernandez, H;Tallman, MS;Greally, JM;Carraway, H;Licht, JD;Gore, SD;Melnick, A;MDS and secondary AML display unique patterns and abundance of aberrant DNA methylationBlood19652201Support Laboratory, Cincinnati Children's Hospital, and 4 from Allcells. Matched CD34+ and CD34− fractions were obtained from an additional 4 healthy donors through the Stem Cell and Xenograft Core, University of Pennsylvania School of MedicineFull Paper
2009Hasstedt, SJ;Bezemer, ID;Callas, PW;Vossen, CY;Trotman, W;Hebbel, RP;Demers, C;Rosendaal, FR;Bovill, EG;Cell adhesion molecule 1: a novel risk factor for venous thrombosisBlood19643986cover slips coated with gelatin (Allcells Inc) under sterile conditions at 37C, 5% CO 2 in HUVEC Basal Medium containing HUVEC Basal Medium Supplement (Allcells Inc). Cells were SaveFull Paper
2009Shi, P;Lai, R;Lin, Q;Iqbal, AS;Young, LC;Kwak, LW;Ford, RJ;Amin, HM;IGF-IR tyrosine kinase interacts with NPM-ALK oncogene to induce survival of T-cell ALK+ anaplastic large-cell lymphoma cellsBlood19423729Type I insulin-like growth factor receptor (IGF-IR) tyrosine kinase plays important roles in the pathogenesis of several malignancies. Although it promotes the growth of stimulated SaveFull Paper
2009Loh, YH;Agarwal, S;Park, IH;Urbach, A;Huo, H;Heffner, GC;Kim, K;Miller, JD;Ng, K;Daley, GQ;Generation of induced pluripotent stem cells from human bloodBlood19299331obtained from Allcells (Emeryville, CA) were collected from a 26-year-old male donor, and CD34+ cells (mPB014F) were isolated. Generation of CD34 iPS cells was performed using a protocol modified from previously published methods.9Full Paper
2009Nahimana, A;Attinger, A;Aubry, D;Greaney, P;Ireson, C;Thougaard, AV;Tjørnelund, J;Dawson, KM;Dupuis, M;Duchosal, MA;The NAD biosynthesis inhibitor APO866 has potent antitumor activity against hematologic malignanciesBlood19196867APO866 inhibits nicotinamide phosphoribosyltransferase (NMPRTase), a key enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis from the natural precursor SaveFull Paper
2009Nardi, V;Naveiras, O;Azam, M;Daley, GQ;ICSBP-mediated immune protection against BCR-ABL-induced leukemia requires the CCL6 and CCL9 chemokinesBlood19171873eripheral blood mononuclear cells from healthy volunteers were purchased from AllCells (Emeryville, CA) and maintained in complete RPMI medium. Murine and human cells were treated with IFN alpha 2A or IFN beta (R&D SystemsFull Paper
2009Figueroa, ME;Wouters, BJ;Skrabanek, L;Glass, J;Li, Y;Erpelinck-Verschueren, CA;Langerak, AW;Löwenberg, B;Fazzari, M;Greally, JM;Valk, PJ;Melnick, A;Delwel, R;Genome-wide epigenetic analysis delineates a biologically distinct immature acute leukemia with myeloid/T-lymphoid featuresBlood191687924 acquired from the Translational Trials Development and Support Laboratory, Cincinnati Children's Hospital (Cincinnati, OH) and 4 purchased from Allcells (Emeryville, CA). This research was approved by the institutional review boards at Albert EinstFull Paper
2009Chen, H;Campbell, RA;Chang, Y;Li, M;Wang, CS;Li, J;Sanchez, E;Share, M;Steinberg, J;Berenson, A;Shalitin, D;Zeng, Z;Gui, D;Perez-Pinera, P;Berenson, RJ;Said, J;Bonavida, B;Deuel, TF;Berenson, JR;Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: a novel mechanism of tumor-induced vasculogenesisBlood19060246The human RPMI8226 and U266 MM cell lines and monocyte (THP-1) and myelomonocytic (U937) cell lines were obtained from ATCC (Manassas, VA). Normal human BM was purchased from Allcells (Berkeley, CA). Highly purified human T cells from healthy donorsFull Paper
2009Luo, XM;Maarschalk, E;O'Connell, RM;Wang, P;Yang, L;Baltimore, D;Engineering human hematopoietic stem/progenitor cells to produce a broadly neutralizing anti-HIV antibody after in vitro maturation to human B lymphocytesBlood1905987690 minutes at 50 000 × g. Human cord blood CD34+ HSPCs were purchased from AllCells (Emeryville, CA) and maintained for less than 24 hours before infection in Iscove modified Dulbecco medium containing 10% fetal bovine serum and cytokines. The cytokiFull Paper
2009Joshi, I;Minter, LM;Telfer, J;Demarest, RM;Capobianco, AJ;Aster, JC;Sicinski, P;Fauq, A;Golde, TE;Osborne, BA;Notch signaling mediates G1/S cell-cycle progression in T cells via cyclin D3 and its dependent kinasesBlood19001083Notch signaling is critical for the regulation of differentiation, proliferation and apoptosis in many cell types. Notch receptors and various Notch ligands have been shown to have a SaveFull Paper
2009Bedi, R;Du, J;Sharma, AK;Gomes, I;Ackerman, SJ;Human C/EBP-epsilon activator and repressor isoforms differentially reprogram myeloid lineage commitment and differentiationBlood18832658Cord blood (CB) CD34+ cells ( 97% pure) purchased from AllCells (Emeryville, CA) were prestimulated for 18 hours at 106 cells/mL in presence of 100 ng/mL recombinant human stem cell factor (rh-SCF), recombinant human fms-like tyrosine kinase 3 ligandFull Paper
2009Oda, M;Glass, JL;Thompson, RF;Mo, Y;Olivier, EN;Figueroa, ME;Selzer, RR;Richmond, TA;Zhang, X;Dannenberg, L;Green, RD;Melnick, A;Hatchwell, E;Bouhassira, EE;Verma, A;Suzuki, M;Greally, JM;High-resolution genome-wide cytosine methylation profiling with simultaneous copy number analysis and optimization for limited cell numbersNucleic acids research19386619One million fresh CD34 + bone marrow progenitors were purchased from Allcells (Emerville, CA). DNA was extracted from these cells and checked for purity and amount by SaveFull Paper
2009O'Brien, JJ;Baglole, CJ;Garcia-Bates, TM;Blumberg, N;Francis, CW;Phipps, RP;15-deoxy-Delta12,14 prostaglandin J2-induced heme oxygenase-1 in megakaryocytes regulates thrombopoiesisJournal of thrombosis and haemostasis : JTH18983509Human CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5×105 cells per well in a 12-well plate and cultured in serum-free medium as previously described (19) and supplemented with 100 ng/mL of recombinant huFull Paper
2009Liu, PC;Caulder, E;Li, J;Waeltz, P;Margulis, A;Wynn, R;Becker-Pasha, M;Li, Y;Crowgey, E;Hollis, G;Haley, P;Sparks, RB;Combs, AP;Rodgers, JD;Burn, TC;Vaddi, K;Fridman, JS;Combined inhibition of Janus kinase 1/2 for the treatment of JAK2V617F-driven neoplasms: selective effects on mutant cells and improvements in measures of disease severityClinical cancer research : an official journal of the American Association for Cancer Research19887489Mononuclear cells were isolated from peripheral blood samples from patients with PV or normal control individuals by centrifugation through Ficoll (Allcells, Inc.). Colony forming assays SaveFull Paper
2009Cardarelli, PM;Moldovan-Loomis, MC;Preston, B;Black, A;Passmore, D;Chen, TH;Chen, S;Liu, J;Kuhne, MR;Srinivasan, M;Assad, A;Witte, A;Graziano, RF;King, DJ;In vitro and in vivo characterization of MDX-1401 for therapy of malignant lymphomaClinical cancer research : an official journal of the American Association for Cancer Research19401346ADCC assay. Human peripheral blood mononuclear effector cells were purified from either heparinized whole blood (AllCells) or buffy coat (Stanford Blood Center) by standard Ficoll-Paque separation. Cells (2 × 106) were washed in PBS and sent for genoFull Paper
2009Zhong, C;Qu, X;Tan, M;Meng, YG;Ferrara, N;Characterization and regulation of bv8 in human blood cellsClinical cancer research : an official journal of the American Association for Cancer Research19336519Isolation of whole human bone marrow cells from fresh bone marrow collections. Fresh bone marrow samples were obtained from ALLCELLS. The bone marrow was first diluted with 10% fetal bovine serum-containing DMEM and then passed through a 40-μm cell sFull Paper
2009Ara, T;Song, L;Shimada, H;Keshelava, N;Russell, HV;Metelitsa, LS;Groshen, SG;Seeger, RC;DeClerck, YA;Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cellsCancer research19118018Human BMSC were either purchased from ALLCells LLC or obtained from bone marrow samples of patients with neuroblastoma enrolled by the Children's Oncology Group. These SaveFull Paper
2009Senyuk, V;Rinaldi, CR;Li, D;Cattaneo, F;Stojanovic, A;Pane, F;Du, X;Mahmud, N;Dickstein, J;Nucifora, G;Consistent up-regulation of Stat3 Independently of Jak2 mutations in a new murine model of essential thrombocythemiaCancer research19118011Mononuclear cells were separated from the BM aspirates by Ficoll-Hypaque centrifugation. The samples were obtained according to guidelines established by the University of Illinois at Chicago Institutional Review Board. Healthy donors’ BM cells wereFull Paper
2009Kim, YC;Wu, Q;Chen, J;Xuan, Z;Jung, YC;Zhang, MQ;Rowley, JD;Wang, SM;The transcriptome of human CD34+ hematopoietic stem-progenitor cellsProceedings of the National Academy of Sciences of the United States of America19416867De Novo Collection of CD34+ 3′ ESTs. Bone marrow CD34+ cells of 3 healthy donors were purchased from AllCells. 3′ ESTs were collected by using the GLGI method (18, 19). A total of 10,000 CD34+ SAGE tags identified in a previous study (12) were selectFull Paper
2009Majeti, R;Becker, MW;Tian, Q;Lee, TL;Yan, X;Liu, R;Chiang, JH;Hood, L;Clarke, MF;Weissman, IL;Dysregulated gene expression networks in human acute myelogenous leukemia stem cellsProceedings of the National Academy of Sciences of the United States of America19218430Normal human bone marrow mononuclear cells were either purchased from AllCells or obtained from National Marrow Donor Program discarded filter units. For AML specimens, peripheral blood and/or bone marrow was obtained following informed consent at thFull Paper
2009Bernard, JJ;Seweryniak, KE;Koniski, AD;Spinelli, SL;Blumberg, N;Francis, CW;Taubman, MB;Palis, J;Phipps, RP;Foxp3 regulates megakaryopoiesis and platelet functionArteriosclerosis, thrombosis, and vascular biology19661482Human CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5×105 cells per well in a 12-well plate and cultured in serum-free medium as previously described 12 and supplemented with 100 ng/mL of recombinant humaFull Paper
2009Jekle, A;Chow, E;Kopetzki, E;Ji, C;Yan, MJ;Nguyen, R;Sankuratri, S;Cammack, N;Heilek, G;CD4-BFFI: a novel, bifunctional HIV-1 entry inhibitor with high and broad antiviral potencyAntiviral research19559732obtained from ATCC, Manassas, VA. Human PBMC were obtained from AllCells (Emeryville, CA), stimulated for one day in PBMC media (RPMI-1640 media containing 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 2 mM l-glutamine, 1 mM sodium-pyruvaFull Paper
2009Nakahara, K;Wakasa-Morimoto, C;Kobayashi, M;Miki, S;Noshi, T;Seki, T;Kanamori-Koyama, M;Kawauchi, S;Suyama, A;Fujishita, T;Yoshinaga, T;Garvey, EP;Johns, BA;Foster, SA;Underwood, MR;Sato, A;Fujiwara, T;Secondary mutations in viruses resistant to HIV-1 integrase inhibitors that restore viral infectivity and replication kineticsAntiviral research19027039Passage of HIV-1 in the presence of integrase inhibitors (INIs) generates resistant viruses that have mutations in the integrase region. Integrase-resistant mutations Q148K and Q148R SaveFull Paper
2009Strickland, LA;Ross, J;Williams, S;Ross, S;Romero, M;Spencer, S;Erickson, R;Sutcliffe, J;Verbeke, C;Polakis, P;van Bruggen, N;Koeppen, H;Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinomaThe Journal of pathology19334050consenting donors; bone marrow samples and purified CD34+ cells (AllCells Inc., Berkeley, CA, USA) were received within 5 (unfractionated samples) or 24 (fractionated samples) h of collection. PC3 (CRL-1435) and HPAFII (CRL-2119) cells were obtainedFull Paper
2009Leleu, X;Hunter, ZR;Xu, L;Roccaro, AM;Moreau, AS;Santos, DD;Hatjiharissi, E;Bakthavachalam, V;Adamia, S;Ho, AW;Soumerai, J;Patterson, CJ;Manning, RJ;Hamilton, S;Verselis, S;Fox, E;Carrasco, R;Ghobrial, IM;Treon, SP;Expression of regulatory genes for lymphoplasmacytic cell differentiation in Waldenstrom MacroglobulinemiaBritish journal of haematology19220283years and the male/female ratio was 1·6. All WM patients were previously untreated. Table I summarizes the characteristics of the patients. Bone marrow aspirates from healthy donors were used in comparison (AllCells LLC, Berkeley, CA, USA)Full Paper
2009Krupa, A;Fudala, R;Stankowska, D;Loyd, T;Allen, TC;Matthay, MA;Gryczynski, Z;Gryczynski, I;Mettikolla, YV;Kurdowska, AK;Anti-chemokine autoantibody:chemokine immune complexes activate endothelial cells via IgG receptorsAmerican journal of respiratory cell and molecular biology19109244HUVECs were purchased from Cambrex (Walkersville, MD) or Allcells (Berkeley, CA), and cultured in endothelial basal medium containing endothelial growth factor, hydrocortisone, gentamicin, amphoreticin B, bovine brain extract, heparin, and FBS (EGM-MFull Paper
2009Lee, JH;Ort, T;Ma, K;Picha, K;Carton, J;Marsters, PA;Lohmander, LS;Baribaud, F;Song, XY;Blake, S;Resistin is elevated following traumatic joint injury and causes matrix degradation and release of inflammatory cytokines from articular cartilage in vitroOsteoarthritis and cartilage19095472Human primary monocytes isolated from three normal healthy volunteers were purchased from AllCells, LLC (Emeryville, CA). The cells were treated with recombinant human resistin (Peprotech, Inc, Rocky Hill, NJ; endotoxin level was measured as 0.9 EU/mFull Paper
2009Hashimoto, S;Nishiyama, T;Hayashi, S;Takebe, K;Kanzaki, N;Fujishiro, T;Kuroda, R;Kurosaka, M;224 THE FUNCTION OF p53 ON HUMAN CHONDROCYTE APOPTOSIS IN RESPONSE TO SHEAR STRESSOsteoarthritis and Cartilageated by enzymatic digestion of bone from patients undergoing knee endoprothesis due to arthrosis of the knee joint. Human macrophages were differentiated from CD14+ peripheral blood monocytes (Allcells) by treatment with macrophage colonyFull Paper
2009Peterson, SJ;Kim, DH;Li, M;Positano, V;Vanella, L;Rodella, LF;Piccolomini, F;Puri, N;Gastaldelli, A;Kusmic, C;L'Abbate, A;Abraham, NG;The L-4F mimetic peptide prevents insulin resistance through increased levels of HO-1, pAMPK, and pAKT in obese miceJournal of lipid research19224872at various time points (0-120 min) for measurement of blood glucose levels. Human bone marrow-derived mesenchymal stem cells and adipocytes Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing the cells,Full Paper
2009Patel, E;Wang, B;Lien, L;Wang, Y;Yang, LJ;Moreb, JS;Chang, LJ;Diverse T-cell differentiation potentials of human fetal thymus, fetal liver, cord blood and adult bone marrow CD34 cells on lentiviral Delta-like-1-modified mouse stromal cellsImmunology19740310Invitrogen, Carlsbad, CA) supplemented with 20% fetal bovine serum and 1% penicillin-streptomycin. The adult BM and CB CD34+ cells were purchased from AllCells, LLC (Emeryville, CA), and Cambrex Corp. (Baltimore, MD) Aborted fetal liver and thymus tiFull Paper
2009Sutherland, MK;Yu, C;Lewis, TS;Miyamoto, JB;Morris-Tilden, CA;Jonas, M;Sutherland, J;Nesterova, A;Gerber, HP;Sievers, EL;Grewal, IS;Law, CL;Anti-leukemic activity of lintuzumab (SGN-33) in preclinical models of acute myeloid leukemiamAbs20065652Primary normal human monocytes and primary AML patient bone marrow samples were obtained from AllCells (Emeryville, CA). Fluorochrome-conjugated antibodies against human SaveFull Paper
2009Nan, JL;Li, JJ;He, JG;C-reactive protein decreases interleukin-8 production in human endothelial progenitor cells by inhibition of p38 MAPK pathwayChinese medical journal19781372Theabilityto modulate the antigenic pbenotype in response to the environmentiscommon to allcells.ECsmay regulate 1evelsofvascularendothelialgrowthfactorreceptor一2 in response to SaveFull Paper
2009Patterson, ST;Li, J;Kang, JA;Wickrema, A;Williams, DB;Reithmeier, RA;Loss of specific chaperones involved in membrane glycoprotein biosynthesis during the maturation of human erythroid progenitor cellsThe Journal of biological chemistry19258325hematopoietic progenitors were either purchased from Stem Cell Technologies (mPB015F) or initially isolated from growth factor-mobilized peripheral blood (purchased from AllCells, SaveFull Paper
2009Ji, C;Kopetzki, E;Jekle, A;Stubenrauch, KG;Liu, X;Zhang, J;Rao, E;Schlothauer, T;Fischer, S;Cammack, N;Heilek, G;Ries, S;Sankuratri, S;CD4-anchoring HIV-1 fusion inhibitor with enhanced potency and in vivo stabilityThe Journal of biological chemistry19097993In this study, we describe a novel CD4-targeting bifunctional human immunodeficiency virus (HIV-1) fusion inhibitor (CD4-BFFI) that blocks HIV-1 entry by inhibiting both HIV-1 SaveFull Paper
2009Hirschler-Laszkiewicz, I;Tong, Q;Conrad, K;Zhang, W;Flint, WW;Barber, AJ;Barber, DL;Cheung, JY;Miller, BA;TRPC3 activation by erythropoietin is modulated by TRPC6The Journal of biological chemistry19074769Human erythroid progenitors were also cultured using a two-phase liquid culture system (44, 45). Cells were harvested at day 8 of phase II and were predominantly proerythroblasts or basophilic normoblasts. CD34+ cells were purchased from AllCells, LLFull Paper
2009Zhang, Q;Shi, S;Liu, Y;Uyanne, J;Shi, Y;Shi, S;Le, AD;Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitisJournal of immunology (Baltimore, Md. : 1950)19923445Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells and cultured with α-MEM supplemented with 10% FBS, 100 μM l-ascorbic acid-2-phosphate, 2 mM l-glutamine, 100 U/ml penicillin, and 100 μg/ml streptFull Paper
2009Parrish, YK;Baez, I;Milford, TA;Benitez, A;Galloway, N;Rogerio, JW;Sahakian, E;Kagoda, M;Huang, G;Hao, QL;Sevilla, Y;Barsky, LW;Zielinska, E;Price, MA;Wall, NR;Dovat, S;Payne, KJ;IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrowJournal of immunology (Baltimore, Md. : 1950)19299724CB was collected according to guidelines approved by the Institutional Review Boards at Childrens Hospital Los Angeles and Loma Linda University. Adult human BM was purchased from AllCells, LLC, Berkeley, CA; the National Disease Research InterchangeFull Paper
2009Vendel, AC;Calemine-Fenaux, J;Izrael-Tomasevic, A;Chauhan, V;Arnott, D;Eaton, DL;B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNKJournal of immunology (Baltimore, Md. : 1950)19155498Tonsil tissue (AllCells) was dissociated and a single-cell suspension was stained with anti-CD19, anti-IgD, and anti-CD38. Naive tonsillar B cells were identified as CD19 + IgD + CD38 SaveFull Paper
2009Panetta, NJ;Gupta, DM;Kwan, MD;Wan, DC;Commons, GW;Longaker, MT;Tissue harvest by means of suction-assisted or third-generation ultrasound-assisted lipoaspiration has no effect on osteogenic potential of human adipose-derived stromal cellsPlastic and reconstructive surgery19568046Commercially available bone marrow mesenchymal stem cells (AllCells), subjected to similar experimental osteogenic differentiation conditions, demonstrated significantly less staining SaveFull Paper
2009Weigel-Van Aken, KA;Pharmacological activation of guanine nucleotide exchange factors for the small GTPase Rap1 recruits high-affinity beta1 integrins as coreceptors for parvovirus B19: improved ex vivo gene transfer to human erythroid progenitor cellsHuman gene therapy19702438Human bone marrow-derived CD36 + erythroid progenitor cells were purchased from AllCells (Emeryville, CA). Cells were cultured overnight in serum-free medium containing stem cell SaveFull Paper
2009Zinckgraf, JW;Sposato, M;Zielinski, V;Powell, D;Treanor, JJ;von Hofe, E;Identification of HLA class II H5N1 hemagglutinin epitopes following subvirion influenza A (H5N1) vaccinationVaccine19596415Since we did not have access to pre-vaccine samples, eight random naive donor PBMC samples (AllCells, Emeryville, CA) were utilized to assess potential cross-reactive T cell SaveFull Paper
2009Morizono, K;Pariente, N;Xie, Y;Chen, IS;Redirecting lentiviral vectors by insertion of integrin-tageting peptides into envelope proteinsThe journal of gene medicine19434609Human umbilical vein endothelial cells (HUVEC) were purchased from AllCells (Emeryville, CA, USA) and Lonza (Walkersville, MD, USA). HUVEC were cultured in EBM-2 (Lonza) supplemented with EGM-2 singlequots (Lonza). When HUVEC started growing slowly,Full Paper
2009Liang, M;Pariente, N;Morizono, K;Chen, IS;Targeted transduction of CD34+ hematopoietic progenitor cells in nonpurified human mobilized peripheral blood mononuclear cellsThe journal of gene medicine19152374Cells binding with the anti-CD34 antibody were separated by a MACS machine (Miltenyi Biotec). Human primary PBMCs were isolated from leukopacks, Ficoll purified and cultured in RPMI with 20% FCS (Omega Scientific, Tarzana, CA, USA). Frozen human mobiFull Paper
2009Lee, CH;Marion, NW;Hollister, S;Mao, JJ;Tissue formation and vascularization in anatomically shaped human joint condyle ectopically in vivoTissue engineering. Part A19563263hMSCs were isolated from fresh bone marrow samples of multiple anonymous adult donors (AllCells, Berkeley, CA) per our prior methods. Purification of hMSCs was accomplished by SaveFull Paper
2009李文芳, ;黄元庆, ;基于虚拟仪器和 DDE 发电机组温度实时数据采集系统的研制厦门大学学报 (自然科学版)其对应的 DDE 服务名,主题名和项名应用与服务器端一致, 分 别是"wd" ,"DDE wd Test"和"AllCells" .在LabWindows/CVI 实时数据库中, 操作的对象是点AllCells , 是一组油温参数的集合, 可以 SaveFull Paper
2009Hall, AA;Leonardo, CC;Collier, LA;Rowe, DD;Willing, AE;Pennypacker, KR;Delayed treatments for stroke influence neuronal death in rat organotypic slice cultures subjected to oxygen glucose deprivationNeuroscience19723565HUCBC (AllCells, LLC, Inc.) were thawed and resuspended in DMEM containing DNase (1ug/ml) to prevent aggregation. HUCBC were washed twice and resuspended at a concentration of 108 cells/ml. Cultures were maintained in a standard tissue culture incubaFull Paper
2009Fukushima-Shintani, M;Suzuki, K;Iwatsuki, Y;Abe, M;Sugasawa, K;Hirayama, F;Kawasaki, T;Nakahata, T;AKR-501 (YM477) a novel orally-active thrombopoietin receptor agonistEuropean journal of haematology19183407Human cord blood (CB) CD34+ cells (AllCells, Berkeley, CA, USA) at a density of 2500 cells/0.75 mL were suspended in MegaCult™-C (StemCell Technologies Inc., Vancouver, Canada) containing either AKR-501 dissolved in DMSO or rhTPO, and plated in two-wFull Paper
2009Zhao, Z;He, X;Bi, Y;Xia, Y;Tao, N;Li, L;Ma, Q;Induction of CYP4F3 by benzene metabolites in human white blood cells in vivo in human promyelocytic leukemic cell lines and ex vivo in human blood neutrophilsDrug metabolism and disposition: the biological fate of chemicals19029204Whole blood from a healthy male adult was purchased from AllCells, LLC (Emeryville, CA). Neutrophils were isolated according a protocol provided by AllCells, LLC. In brief, whole SaveFull Paper
2009Nishino, T;Miyaji, K;Ishiwata, N;Arai, K;Yui, M;Asai, Y;Nakauchi, H;Iwama, A;Ex vivo expansion of human hematopoietic stem cells by a small-molecule agonist of c-MPLExperimental hematology197445390.5 U/mL rhEPO, and 10 ng/mL rhTPO, respectively. Human cord blood (hCB) CD34+ cells were purchased from Lonza (Basel, Switzerland) and AllCells (Berkeley, CA, USA) or purified from hCB. Fresh hCB cells were obtained from the Tokyo Cord Blood Bank (TFull Paper
2009Giammona, LM;Panuganti, S;Kemper, JM;Apostolidis, PA;Lindsey, S;Papoutsakis, ET;Miller, WM;Mechanistic studies on the effects of nicotinamide on megakaryocytic polyploidization and the roles of NAD+ levels and SIRT inhibitionExperimental hematology19715739Cultures were initiated in T-flasks with previously frozen mPB CD34-selected cells (AllCells; Berkeley, CA or Fred Hutchinson Cancer Research Center; Seattle, WA) and maintained at a concentration of 100,000 to 300,000 cells/ml as described [8]. MostFull Paper
2009Gibson, JD;Jakuba, CM;Boucher, N;Holbrook, KA;Carter, MG;Nelson, CE;Single-cell transcript analysis of human embryonic stem cellsIntegrative biology : quantitative biosciences from nano to macro20023769We demonstrate the qualitative and quantitative power of single-cell transcript analysis to characterize transcriptome dynamics in human embryonic stem cells (hESC's). Single-cell SaveFull Paper
2009Wells, R;Guth, A;Lappin, M;Dow, S;Anti-endothelial cell antibodies in dogs with immune-mediated hemolytic anemia and other diseases associated with high risk of thromboembolismJournal of veterinary internal medicine19192144AllCells LLC, Emeryville, CA bSigma Aldrich, St Louis, MO cGibco-InVitrogen, San Diego, CA dHyclone, Logan, UT eJackson ImmunoResearch, West Grove, PA fDako-Cytomation, Fort Collins, COFull Paper
2009Harriman, WD;Collarini, EJ;Cromer, RG;Dutta, A;Strandh, M;Zhang, F;Kauvar, LM;Multiplexed Elispot assayJournal of immunological methods19084532Normal human PBMCs were obtained from AllCells (Emeryville, CA). For CellSpot assays, 105 PBMC, Th1 or Th2 selected cells were suspended in activation medium with phorbol myristate acetate (PMA) at 50 ng/mL and Ionomycin at 1 µg/mL or control medium,Full Paper
2009Sugiyama, E;Lee, SJ;Lee, SS;Kim, WY;Kim, SR;Tohkin, M;Hasegawa, R;Okuda, H;Kawamoto, M;Kamatani, N;Sawada, J;Kaniwa, N;Saito, Y;Shin, JG;Ethnic differences of two non-synonymous single nucleotide polymorphisms in CDA geneDrug metabolism and pharmacokinetics20045991DNA from 200 healthy Chinese-Americans was extracted from cord blood samples purchased from AllCells (Emeryville, CA, USA). Peripheral blood samples from healthy Caucasian- SaveFull Paper
2009Ye, D;Garcia-Manero, G;Kantarjian, HM;Xiao, L;Vadhan-Raj, S;Fernandez, MH;Nguyen, MH;Medeiros, LJ;Bueso-Ramos, CE;Analysis of Aurora kinase A expression in CD34(+) blast cells isolated from patients with myelodysplastic syndromes and acute myeloid leukemiaJournal of hematopathology19669217reverse transcription efficiency. Relative RNA level was reported via standard delta delta Ct (δδ Ct). cDNA purified from normal CD34+ hematopoietic progenitor cells was used as calibrator sample (Allcells, LLC, Emeryville, CA, USA). ImmunocytochemiFull Paper
2009Nagel, I;Identifizierung von pathogenetisch relevanten Genen bei lymphatischen Neoplasien unter besonderer Berücksichtigung des langen Arms von Chromosom 14ThesisProbanden isoliert. Zur Normalisierung der quantitativen Reverse Transkription (RT)-PCR-Analyse des TRAF3-Gens wurde „Normal Peripheral Blood B Cells cDNA (CD19+)“ der Firma Allcells (Emeryville, CA, USA) verwendet. Für die quantitativen RT-PCR-AnalyFull Paper
2009Mahadevan, D;Choi, J;Cooke, L;Simons, B;Riley, C;Klinkhammer, T;Sud, R;Maddipoti, S;Hehn, S;Garewal, H;Spier, C;Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3 and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival and MigrationHuman genomics and proteomics : HGP20981323CD19+ normal PB B-cell RNA from 4 normal volunteers was purchased from AllCells, CA. Control RNA was obtained from normal peripheral (PB) B-cells from 4 volunteers (AllCells, CA) SaveFull Paper
2009Garzon, R;MicroRNA profiling of megakaryocytesMethods in molecular biology (Clifton, N.J.)18839118It is critical to use high-quality CD34+ stem cells. These cells could be obtained commercially from Allcells.com or Stemcell technologies. Other cheaper alternatives include the use of cord blood-selected CD34+ cells. Failure to use high-quality proFull Paper
2009Huynh, H;Kaba, M;Rudra, S;Zheng, J;Wu, C;Lodish, H;Zhang, C;IGFBP2 Supports ex vivo Expansion of Hematopoietic Stem CellsResearch and Perspectives in Endocrine InteractionsFresh and cryopreserved human cord blood cells were purchased from Cambrex, StemCell Technologies Inc., and AllCells. All of the cells were from pooled donors. CD34 + or CD133 SaveFull Paper
2009Harirchian, P;Validation of EBI2 as a Novel Therapeutic Target for Autoimmune DisordersThesisFresh leukapheresed blood (300 mL) from healthy donors was purchased from Allcells (Emeryville, CA). On the same day of leukapheresis, peripheral blood mononuclear cells (PBMC) SaveFull Paper
2009Bachstetter, AD;The role of neuroinflammation in regulating the age-related decline in neurogenesisThesisCell preparation: Cryopreserved human umbilical cord blood mononuclear cells (UCBMCs) were obtained from Saneron CCEL Therapeutics, Inc. (Tampa, FL, USA). Cryopreserved Human peripheral blood cells (PBMC) (mononuclear fraction) were obtained from AllFull Paper
2009Carter, CC;Infection of hematopoietic progenitor cells by HIV-1ThesisCell culture. Fresh whole bone marrow aspirates were obtained commercially (AllCells Ltd.). Umbilical cord blood was obtained following scheduled cesarean section procedures, in accordance with an IRB-approved protocol. Bone marrow mononuclear cellsFull Paper
2009O'Brien, JJ;Mechanisms of megakaryopoiesis and platelet production: Implications for inflammation and diseaseThesisHuman CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5x10" cells per well in a 12-well plate and cultured in serum-free medium as previously described (Zeuner et al. 2007) and supplemented with 100 ng/mL oFull Paper
2009Hall, AA;Immunomodulatory effects of novel therapies for strokeThesisDMEM without glucose (Invitrogen, Carlsbad, CA) for OGD. HUCBC (AllCells) were thawed and resuspended in DMEM containing DNase (1ug/ml) to prevent aggregation. HUCBC were washed twice and resuspended at a concentration of 108 cells/ml. HUCBC (106 )Full Paper
2009Schwengberg, S;Use of embryonic stem cell derived cardiomyocytes for determination of cardiac cytotoxicityJournal of Pharmacological and Toxicological MethodsHescheler for providing Patch Clamp analysis; Leonard Fink for development and programming of the automated analysis software; our colleagues at Axiogenesis AG and ReachBio SaveFull Paper
2008Ramalingam, S;Forster, J;Naret, C;Evans, T;Sulecki, M;Lu, H;Teegarden, P;Weber, MR;Belani, CP;Dual inhibition of the epidermal growth factor receptor with cetuximab, an IgG1 monoclonal antibody, and gefitinib, a tyrosine kinase inhibitor, in patients with refractory non-small cell lung cancer (NSCLC): a phase I studyJ Thorac Oncol1831706838. FFPE tumor samples were submitted to QualTek (Newtown, PA) for EGFR expression analysis by immunohistochemistry. The intensity of staining was scored as: 3 = high, 2 = moderate, 1 = low/minimal, and 0 = no staining. RESULTS. Patient DemographicsFull Paper
2008Jiang, P;Enomoto, A;Jijiwa, M;Kato, T;Hasegawa, T;Ishida, M;Sato, T;Asai, N;Murakumo, Y;Takahashi, M;An actin-binding protein Girdin regulates the motility of breast cancer cellsCancer Res.18316593Tissue arrays of human malignant tumors and matched normal adjacent tissues were purchased from Folio Biosciences, Inc. Immunohistochemisty was performed using standard techniques. Antigen retrieval was performed with microwave treatment in a 0.01 moFull Paper
2008Lu, Y;Wang, J;Xu, Y;Koch, AE;Cai, Z;Chen, X;Galson, DL;Taichman, RS;Zhang, J;CXCL16 functions as a novel chemotactic factor for prostate cancer cells in vitroMol. Cancer Res.18344492High-density tissue microarrays were constructed by Folio Biosciences with clinical samples obtained from a cohort of 80 patients. Tumors were graded using the Gleason grading system and examined to identify areas of benign prostate, prostate cancer,Full Paper
2008Wang, J;Shiozawa, Y;Wang, J;Wang, Y;Jung, Y;Pienta, KJ;Mehra, R;Loberg, R;Taichman, RS;The role of CXCR7/RDC1 as a chemokine receptor for CXCL12/SDF-1 in prostate cancerJ. Biol. Chem.18057003tumor size, or clinical outcome. Breast cancer arrays were purchased from Folio Biosciences (Columbus, OH) (ARY-HH0058), which were generated from clinical samples derived from a cohort of 80 patients. In this case anFull Paper
2008Gallagher, J;Lynch, FW;Barragry, J;A prolactinoma masked by a herbal remedyEur. J. Obstet. Gynecol. Reprod. Biol.17298863Full Paper
2008Meszaros, R;Strate, I;Pera, EM;Durbeej, M;Expression of the novel gene Ened during mouse and Xenopus embryonic developmentInt. J. Dev. Biol.18956345Mul- tiple alignment of the amino acid sequences was carried out with MultAlin software (http://bioinfo.genopole-toulouse.prd.fr/multalin/multalin.html). Whole-mount and radioactive in situ hybridization (Phylogeny, Inc. ColumbusFull Paper
2008Kawada, M;Inoue, H;Arakawa, M;Ikeda, D;Transforming growth factor-β1 modulates tumor-stromal cell interactions of prostate cancer through insulin-like growth factor-IAnticancer researchImmunohistochemistry. Tissue arrays of human prostate cancer were purchased from Folio Biosciences (Columbus, OH, USA). The tissue sections were deparaffinized in xylene and rehydrated in descending alcohols into waterFull Paper
2008Cherry, RA;Nichols, PA;Snavely, TM;David, MT;Lynch, FC;Prophylactic inferior vena cava filters: do they make a difference in trauma patients?J Trauma18784566Full Paper
2008Yang, L;Anderson, DE;Baecher-Allan, C;Hastings, WD;Bettelli, E;Oukka, M;Kuchroo, VK;Hafler, DA;IL-21 and TGF-beta are required for differentiation of human T(H)17 cellsNature18469800PBMCs were obtained from the peripheral blood of healthy subjects or from cord blood (AllCells) in compliance with institutional review board (IRB) protocols. CD4 + T cells were SaveFull Paper
2008Cisse, B;Caton, ML;Lehner, M;Maeda, T;Scheu, S;Locksley, R;Holmberg, D;Zweier, C;den Hollander, NS;Kant, SG;Holter, W;Rauch, A;Zhuang, Y;Reizis, B;Transcription factor E2-2 is an essential and specific regulator of plasmacytoid dendritic cell developmentCell18854153Total RNA from purified human peripheral blood cell types (Allcells) or sorted murine cells was reverse transcribed and assayed by SYBR Green-based real-time PCR with MX3000P SaveFull Paper
2008Paoluzzi, L;Gonen, M;Bhagat, G;Furman, RR;Gardner, JR;Scotto, L;Gueorguiev, VD;Heaney, ML;Manova, K;O'Connor, OA;The BH3-only mimetic ABT-737 synergizes the antineoplastic activity of proteasome inhibitors in lymphoid malignanciesBlood18591385Mononuclear cells from peripheral blood samples (PBMCs) of healthy donors were purchased from Allcells (Emeryville, CA). All cell lines were grown as previously described. SaveFull Paper
2008Luqman, M;Klabunde, S;Lin, K;Georgakis, GV;Cherukuri, A;Holash, J;Goldbeck, C;Xu, X;Kadel, EE;Lee, SH;Aukerman, SL;Jallal, B;Aziz, N;Weng, WK;Wierda, W;O'Brien, S;Younes, A;The antileukemia activity of a human anti-CD40 antagonist antibody, HCD122, on human chronic lymphocytic leukemia cellsBlood18497318B-cell chronic lymphocytic leukemia (B-CLL) is a lymphoproliferative disorder characterized by the surface expression of CD20, CD5 antigens, as well as the receptor CD40. Activation SaveFull Paper
2008Zhang, CC;Kaba, M;Iizuka, S;Huynh, H;Lodish, HF;Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantationBlood18202223Fresh and cryopreserved human cord blood cells were purchased from Cambrex (East Rutherford, NJ), StemCell Technologies, and AllCells (Berkeley, CA). All of cells were from pooled donors. CD34+ or CD133+ purity checked by flow cytometry was higher thFull Paper
2008Garzon, R;Volinia, S;Liu, CG;Fernandez-Cymering, C;Palumbo, T;Pichiorri, F;Fabbri, M;Coombes, K;Alder, H;Nakamura, T;Flomenberg, N;Marcucci, G;Calin, GA;Kornblau, SM;Kantarjian, H;Bloomfield, CD;Andreeff, M;Croce, CM;MicroRNA signatures associated with cytogenetics and prognosis in acute myeloid leukemiaBlood18187662from 4 healthy donors were purchased from Allcells (Emeryville, CA). Bone marrow CD34 + progenitors from 10 healthy donors were purchased from Allcells. In vitro differentiated SaveFull Paper
2008Mercher, T;Cornejo, MG;Sears, C;Kindler, T;Moore, SA;Maillard, I;Pear, WS;Aster, JC;Gilliland, DG;Notch signaling specifies megakaryocyte development from hematopoietic stem cellsCell stem cell18786418using a FACSAria (BD Biosciences). Human CD34+ bone marrow cells were obtained from AllCells. For in vitro cocultures of LSK cells with stroma, analyses were gated on forward/side scatter profile and CD45+ or CD45+GFP+ cells. Apoptosis assays were peFull Paper
2008Tuller, T;Kupiec, M;Ruppin, E;Evolutionary rate and gene expression across different brain regionsGenome biology18811952names and other properties appears in Additional data file 12. The human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA, USA), Clontech (Palo Alto, CA, USA), AllCells (Berkeley, CA, USA), Clonetics/BioWhittakFull Paper
2008Song, W;van der Vliet, HJ;Tai, YT;Prabhala, R;Wang, R;Podar, K;Catley, L;Shammas, MA;Anderson, KC;Balk, SP;Exley, MA;Munshi, NC;Generation of antitumor invariant natural killer T cell lines in multiple myeloma and promotion of their functions via lenalidomide: a strategy for immunotherapyClinical cancer research : an official journal of the American Association for Cancer Research18980990Normal bone marrow samples were purchased from AllCells. Multiple myeloma patient blood and bone marrow samples were obtained from Dana-Farber Cancer Institute following SaveFull Paper
2008Lu, J;Guo, S;Ebert, BL;Zhang, H;Peng, X;Bosco, J;Pretz, J;Schlanger, R;Wang, JY;Mak, RH;Dombkowski, DM;Preffer, FI;Scadden, DT;Golub, TR;MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte progenitorsDevelopmental cell18539114Human umbilical cord blood was harvested at Brigham and Women’s Hospital with informed patient consent under an IRB approved protocol. Adult human bone marrow cells were obtained from AllCells, LLC. Mononuclear cells were purified by Ficoll Hypaque sFull Paper
2008Solomon, DA;Kim, JS;Cronin, JC;Sibenaller, Z;Ryken, T;Rosenberg, SA;Ressom, H;Jean, W;Bigner, D;Yan, H;Samuels, Y;Waldman, T;Mutational inactivation of PTPRD in glioblastoma multiforme and malignant melanomaCancer research19074898Normal human astrocytes (NHA) were obtained from Clonetics and AllCells. All cell lines were grown in DMEM + 10% fetal bovine serum (FBS) at 37C in 5% CO 2 . SaveFull Paper
2008Lau, YK;Murray, LB;Houshmandi, SS;Xu, Y;Gutmann, DH;Yu, Q;Merlin is a potent inhibitor of glioma growthCancer research18632626Normal human astrocytes (NHA cells) were obtained from ALLCELLS, Inc. Cells were maintained following the providers' and manufacturers' instructions. Anti-v5 epitope (Invitrogen), SaveFull Paper
2008Solomon, DA;Kim, JS;Jenkins, S;Ressom, H;Huang, M;Coppa, N;Mabanta, L;Bigner, D;Yan, H;Jean, W;Waldman, T;Identification of p18 INK4c as a tumor suppressor gene in glioblastoma multiformeCancer research18381405Normal human astrocytes (NHAs) were obtained from Clonetics and Allcells. All cell lines were grown in DMEM + 10% fetal bovine serum at 37 in 5% CO 2 . SaveFull Paper
2008Bonini, MG;Stadler, K;Silva, SO;Corbett, J;Dore, M;Petranka, J;Fernandes, DC;Tanaka, LY;Duma, D;Laurindo, FR;Mason, RP;Constitutive nitric oxide synthase activation is a significant route for nitroglycerin-mediated vasodilationProceedings of the National Academy of Sciences of the United States of America18562300eNOS (Ser-1177 and Thr-495) were from Santa Cruz and used according to the manufacturer's instructions. HUVECs were from Allcells and cultured as described (24). All other chemicals used were analytical grade or superiorFull Paper
2008O'Hare, T;Eide, CA;Tyner, JW;Corbin, AS;Wong, MJ;Buchanan, S;Holme, K;Jessen, KA;Tang, C;Lewis, HA;Romero, RD;Burley, SK;Deininger, MW;SGX393 inhibits the CML mutant Bcr-AblT315I and preempts in vitro resistance when combined with nilotinib or dasatinibProceedings of the National Academy of Sciences of the United States of America18367669Hematopoietic Colony Forming Assays of Normal Bone Marrow. Bone marrow mononuclear cells (AllCells) from three normal individuals were cultured with SGX393. Cells (5 × 104) were plated in methylcellulose containing 50 ng/ml SCF, 10 ng/ml GM-CSF, andFull Paper
2008Lund, TC;Tolar, J;Orchard, PJ;Granulocyte colony-stimulating factor mobilized CFU-F can be found in the peripheral blood but have limited expansion potentialHaematologica18403392Briefly, blood from G-CSF treated healthy volunteers was purchased from AllCells, LLC (Emeryville, CA, USA) (see Online Supplementary Appendix). The use of all tissue was approved by the Committee on the Use of Human Subjects in Research at the UniveFull Paper
2008Van Scott, MR;Mertsching, E;Negrou, E;Miles, J;Stallings, HW;Graff, C;Kehry, MR;Systemic administration of an Fcgamma-Fc(epsilon)-fusion protein in house dust mite sensitive nonhuman primatesClinical immunology (Orlando, Fla.)18583194Human basophils were differentiated from CD34+ cord blood progenitors (AllCells, LLC, Emeryville, CA) by 4- to 5-weeks of culture in complete medium [RPMI 1640 (ATCC, Manassas, VA), 10% FBS (HyClone, Logan , UT), 10 μg/ml gentamicin (Sigma-Aldrich, SFull Paper
2008Reyna, SM;Ghosh, S;Tantiwong, P;Meka, CS;Eagan, P;Jenkinson, CP;Cersosimo, E;Defronzo, RA;Coletta, DK;Sriwijitkamol, A;Musi, N;Elevated toll-like receptor 4 expression and signaling in muscle from insulin-resistant subjectsDiabetes18633101e performed Western analysis and real-time PCR of EMR1, a marker for monocyte/macrophage infiltration, in muscle tissue of insulin-resistant subjects and in cultured myotubes. Unlike CD14+ monocytes (AllCells, Emeryville, CA), which expressed EMR1, nFull Paper
2008Smith, LM;Nesterova, A;Ryan, MC;Duniho, S;Jonas, M;Anderson, M;Zabinski, RF;Sutherland, MK;Gerber, HP;Van Orden, KL;Moore, PA;Ruben, SM;Carter, PJ;CD133/prominin-1 is a potential therapeutic target for antibody-drug conjugates in hepatocellular and gastric cancersBritish journal of cancer18542072idoma AC133. 1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and normal human primary cells (HREC, hepatocytes) were obtained from Cambrex (Lonza, Switzerland) and AllCells (Emeryville, CA, USA), respectively. CellFull Paper
2008Xu, Y;Theobald, V;Sung, C;DePalma, K;Atwater, L;Seiger, K;Perricone, MA;Richards, SM;Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patientsJournal of translational medicine18945350oulter Immunomics, San Diego, CA). Frozen PBMC Samples: Frozen peripheral blood mononuclear cells (PBMCs), screened HIV negative, were used in this study. PBMC from blood of HLA-A2 healthy subjects (AllCells, LLC, Emeryville, CA and American Red CroFull Paper
2008DiBlasio-Smith, EA;Arai, M;Quinet, EM;Evans, MJ;Kornaga, T;Basso, MD;Chen, L;Feingold, I;Halpern, AR;Liu, QY;Nambi, P;Savio, D;Wang, S;Mounts, WM;Isler, JA;Slager, AM;Burczynski, ME;Dorner, AJ;LaVallie, ER;Discovery and implementation of transcriptional biomarkers of synthetic LXR agonists in peripheral blood cellsJournal of translational medicine18925943Fresh human PBMC, T cells, B cells, and monocytes from normal human donors were purchased from AllCells (Emeryville, CA). Each cell set was derived from the same donor for SaveFull Paper
2008Inamdar, KV;O'Brien, S;Sen, S;Keating, M;Nguyen, MH;Wang, X;Fernandez, M;Thomazy, V;Medeiros, LJ;Bueso-Ramos, CE;Aurora-A kinase nuclear expression in chronic lymphocytic leukemiaModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc18931650fam-ATCAAGATCATTGCTCCTCCTGAGCGC-bhq) at 100 nM concentrations. Real-time quantitative RT-PCR was performed in an automatic MX3000P real-time RT-PCR thermal cycler (Stratagene, La Jolla, CA, USA). Relative RNA level was reported via standard delta delFull Paper
2008Peterson, SJ;Drummond, G;Kim, DH;Li, M;Kruger, AL;Ikehara, S;Abraham, NG;L-4F treatment reduces adiposity, increases adiponectin levels, and improves insulin sensitivity in obese miceJournal of lipid research18426778Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in α-MEM (Invitrogen) supplemented with 10% heat-inactivated FBS (Invitrogen), and 1% antibiotic/antimycotic solutioFull Paper
2008Liebler, JM;Lutzko, C;Banfalvi, A;Senadheera, D;Aghamohammadi, N;Crandall, ED;Borok, Z;Retention of human bone marrow-derived cells in murine lungs following bleomycin-induced lung injuryAmerican journal of physiology. Lung cellular and molecular physiology18515407Human BMDC preparation and administration. Whole adult human bone marrow cells (Cambrex, Walkersville, MD or AllCells, Emeryville, CA) were used as the source of BMDC under a protocol approved by the Institutional Review Board of the University of SoFull Paper
2008Mina-Osorio, P;Winnicka, B;O'Conor, C;Grant, CL;Vogel, LK;Rodriguez-Pinto, D;Holmes, KV;Ortega, E;Shapiro, LH;CD13 is a novel mediator of monocytic/endothelial cell adhesionJournal of leukocyte biology1849578837 cells, WEHI‐274.1 (murine myeloid cell line), C33A cells, and hemangioendothelioma (EOMA) cells [19] were from the American Type Cell Culture Collection (ATCC; Manassas, VA, USA). PBMC were from AllCells (Emeryville, CA, USA). Anti‐CD13 mAb 452 anFull Paper
2008Kopetzki, E;Jekle, A;Ji, C;Rao, E;Zhang, J;Fischer, S;Cammack, N;Sankuratri, S;Heilek, G;Closing two doors of viral entry: intramolecular combination of a coreceptor- and fusion inhibitor of HIV-1Virology journal18452606s were obtained from the NIH AIDS Research and Reference Reagent program. Human embryonic kidney cells 293-T and HEK293-EBNA cells were obtained from ATCC, Manassas, VA. Human PBMC were obtained from AllCells (Emeryville, CA), stimulated for one dayFull Paper
2008Tomchuck, SL;Zwezdaryk, KJ;Coffelt, SB;Waterman, RS;Danka, ES;Scandurro, AB;Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responsesStem cells (Dayton, Ohio)17916800Human MSCs were obtained from our collaborators at the Tulane University Center for Gene Therapy led by Darwin J. Prockop, M.D., Ph.D. Additionally, MSCs were obtained from two commercial suppliers Cambrex BioScience (Walkersville, MD) and Allcells (Full Paper
2008Lee, EJ;Baek, M;Gusev, Y;Brackett, DJ;Nuovo, GJ;Schmittgen, TD;Systematic evaluation of microRNA processing patterns in tissues, cell lines, and tumorsRNA (New York, N.Y.)18025253Human cancer cell lines were obtained from ATCC or other investigators. Human total RNA from 20 tissues was from Ambion. CD19+ human B cells were purchased from AllCells. RNA from pancreas and liver tumor and adjacent benign pancreas were obtained frFull Paper
2008Clark, PA;Moioli, EK;Sumner, DR;Mao, JJ;Porous implants as drug delivery vehicles to augment host tissue integrationFASEB journal : official publication of the Federation of American Societies for Experimental Biology18198217Fresh bone marrow samples of multiple adult male donors (AllCells, Berkeley, CA, USA) were used to isolate MSC, per our previous methods (19, 23). Nonadherent cells were removed SaveFull Paper
2008Mittal, R;Prasadarao, NV;Outer membrane protein A expression in Escherichia coli K1 is required to prevent the maturation of myeloid dendritic cells and the induction of IL-10 and TGF-betaJournal of immunology (Baltimore, Md. : 1950)18684958Dendritic cells (DCs) are professional APCs that direct both cellular and humoral immune responses. Escherichia coli K1 causes meningitis in neonates; however, the interactions SaveFull Paper
2008Fukaya, Y;Shimada, H;Wang, LC;Zandi, E;DeClerck, YA;Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stromaThe Journal of biological chemistry18450743transferrin (0.25 μg/ml), selenium (0.25 ng/ml), and l-glutamine. Human BMSC were obtained from ALLCELLS (Emeryville, CA) and maintained in basal medium with stimulatory supplements containing basic fibroblast growth factor (10 ng/ml)Full Paper
2008Tong, Q;Hirschler-Laszkiewicz, I;Zhang, W;Conrad, K;Neagley, DW;Barber, DL;Cheung, JY;Miller, BA;TRPC3 is the erythropoietin-regulated calcium channel in human erythroid cellsThe Journal of biological chemistry18276585Human erythroid progenitors/precursors were cultured using a two-phase liquid culture system (31, 32). Cells harvested at day 8 of Phase II were predominantly proerythroblasts and basophilic normoblasts. CD34+ cells were purchased from AllCells, LLCFull Paper
2008Wang, M;Windgassen, D;Papoutsakis, ET;Comparative analysis of transcriptional profiling of CD3+, CD4+ and CD8+ T cells identifies novel immune response players in T-cell activationBMC genomics18485203ng regulation of T-cell activation in the natural CD3+ population, as well as in the CD4+ and CD8+ subsets. Methods Cells and culture system Healthy-donor peripheral blood mononuclear cells (PBMCs) (AllCells, Berkeley, CA) were used to negatively-seFull Paper
2008Moioli, EK;Clark, PA;Sumner, DR;Mao, JJ;Autologous stem cell regeneration in craniosynostosisBone18023269Human mesenchymal stem cells isolated from bone marrow (AllCells, Berkeley, CA) were cultured in 6-well plates (3 × 10 4 cells/well) with osteogenic supplements as described above SaveFull Paper
2008Liang, W;Rahaman, M;Day, D;Marion, N;Riley, G;Mao, J;Bioactive borate glass scaffold for bone tissue engineeringJournal of Non-Crystalline SolidsBone marrow derived human mesenchymal stem cells, hbMSCs (AllCells; Berkeley, CA) were cultured for 18 days in either DMEM complete (89% DMEM 10% FBS, 1% penicillin - SaveFull Paper
2008Sikorska, M;Sandhu, JK;Deb-Rinker, P;Jezierski, A;Leblanc, J;Charlebois, C;Ribecco-Lutkiewicz, M;Bani-Yaghoub, M;Walker, PR;Epigenetic modifications of SOX2 enhancers, SRR1 and SRR2, correlate with in vitro neural differentiationJournal of neuroscience research18293417Primary human astrocytes (HA; prepared from fetuses between 9 and 10 week of gestation) were purchased from AllCells LLC. (Emeryville, CA). The cells were cultured in poly-L-lysine-coated T25-cm2 flasks using basal medium containing stimulatory supplFull Paper
2008Papaliodis, D;Boucher, W;Kempuraj, D;Michaelian, M;Wolfberg, A;House, M;Theoharides, TC;Niacin-induced "flush" involves release of prostaglandin D2 from mast cells and serotonin from platelets: evidence from human cells in vitro and an animal modelThe Journal of pharmacology and experimental therapeutics18784348Freshly isolated human platelets were purchased from AllCells, LLC (Emeryville, CA) and sent to us overnight. Platelets were used the day of arrival after washing as indicated below. SaveFull Paper
2008Kim, DH;Burgess, AP;Li, M;Tsenovoy, PL;Addabbo, F;McClung, JA;Puri, N;Abraham, NG;Heme oxygenase-mediated increases in adiponectin decrease fat content and inflammatory cytokines tumor necrosis factor-alpha and interleukin-6 in Zucker rats and reduce adipogenesis in human mesenchymal stem cellsThe Journal of pharmacology and experimental therapeutics18334666Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential medium ( SaveFull Paper
2008Fuhrken, PG;Chen, C;Apostolidis, PA;Wang, M;Miller, WM;Papoutsakis, ET;Gene Ontology-driven transcriptional analysis of CD34+ cell-initiated megakaryocytic cultures identifies new transcriptional regulators of megakaryopoiesisPhysiological genomics18252802Mk and G cultures were initiated with fresh (AllCells; Berkeley, CA) or frozen (Hematopoietic Cell Processing Core, Fred Hutchinson Cancer Center, Seattle, WA) granulocyte colony-stimulating factor (G-CSF)-mobilized human peripheral blood CD34+ cellsFull Paper
2008Stirewalt, DL;Meshinchi, S;Kopecky, KJ;Fan, W;Pogosova-Agadjanyan, EL;Engel, JH;Cronk, MR;Dorcy, KS;McQuary, AR;Hockenbery, D;Wood, B;Heimfeld, S;Radich, JP;Identification of genes with abnormal expression changes in acute myeloid leukemiaGenes, chromosomes & cancer17910043Cancer Research Center (FHCRC) or purchased from commercially available vendors (Cambrex, Rutherford, NJ or AllCells, Emeryville, CA). BM (N = 7) and PB (N = 19) samples from 26 adult AML patients were obtained from the FHCRC's Leukemia Repository. TFull Paper
2008Li, YY;Bao, M;Meurer, J;Skuballa, W;Bauman, JG;Doecke, WD;Zollner, TM;The identification of a small molecule inhibitor that specifically reduces T cell-mediated adaptive but not LPS-mediated innate immunity by T cell membrane-monocyte contact bioassayImmunology letters18241931PBMCs were either purchased (Allcells, Emeryville, CA) or isolated from healthy donor blood after informed consent. Berlex environmental health & safety (EHS) policies and SaveFull Paper
2008Ramsubir, S;Nonaka, T;Girbés, CB;Carpentier, S;Levade, T;Medin, JA;In vivo delivery of human acid ceramidase via cord blood transplantation and direct injection of lentivirus as novel treatment approaches for Farber diseaseMolecular genetics and metabolism18805722CD34+ cells were obtained from AllCells, LLC (Berkeley, CA). Cells were pre-stimulated for 12 h in StemSpan SFEM medium (Stem Cell Technologies, Vancouver, BC, Canada) supplemented with recombinant human SCF, Flt3-L, thrombopoietin (all at 50 ng/ml),Full Paper
2008Huang, D;Pereboev, AV;Korokhov, N;He, R;Larocque, L;Gravel, C;Jaentschke, B;Tocchi, M;Casley, WL;Lemieux, M;Curiel, DT;Chen, W;Li, X;Significant alterations of biodistribution and immune responses in Balb/c mice administered with adenovirus targeted to CD40(+) cellsGene therapy18046426using Adeno-X Rapid Titer kit according to the manufacturer's instructions (BD Biosciences, San Jose, CA, USA). DC culture, Ad infection and luciferase activity assay Myeloid DCs were obtained from AllCELLS, Emeryville, CA, USA. Over 80% of these moFull Paper
2008Oh, W;Kim, DS;Yang, YS;Lee, JK;Immunological properties of umbilical cord blood-derived mesenchymal stromal cellsCellular immunology18495100To assess T-cell reactivity against allogeneic cell populations, human responder PBMCs (1 × 105/well) were cocultured with inactivated allogeneic PBMCs (1 × 105/well) or hUCB-MSCs (1 × 102, 1 × 103 or 1 × 104/well) in 96-well tissue culture plates. TFull Paper
2008Seo, BM;Sonoyama, W;Yamaza, T;Coppe, C;Kikuiri, T;Akiyama, K;Lee, JS;Shi, S;SHED repair critical-size calvarial defects in miceOral diseases18938268Human bone marrow cells were purchased from commercially available resources (AllCells LLC, Berkeley, CA, USA). To identify putative bone marrow mesenchymal stem cells, single SaveFull Paper
2008Moioli, EK;Clark, PA;Chen, M;Dennis, JE;Erickson, HP;Gerson, SL;Mao, JJ;Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissuesPloS one19081793Bone marrow cells were isolated from whole marrow aspirates of the iliac crest of each of three healthy male donors (AllCells, Berkeley, CA) and plated as in Fig. 1A. Human SaveFull Paper
2008Yamaza, T;Miura, Y;Bi, Y;Liu, Y;Akiyama, K;Sonoyama, W;Patel, V;Gutkind, S;Young, M;Gronthos, S;Le, A;Wang, CY;Chen, W;Shi, S;Pharmacologic stem cell based intervention as a new approach to osteoporosis treatment in rodentsPloS one18612428amethasone (Sigma-Aldrich Co.) were added into the culture for 6 weeks. Human BMMSC culture Human bone marrow aspirates from healthy human adult volunteers (20-35 years of age) were purchased from AllCells LLC and cultured as reported previously [21Full Paper
2008Ghosh, A;Park, JY;Fenno, C;Kapila, YL;Porphyromonas gingivalis, gamma interferon, and a proapoptotic fibronectin matrix form a synergistic trio that induces c-Jun N-terminal kinase 1-mediated nitric oxide generation and cell deathInfection and immunity18838522Human primary normal peripheral blood neutrophils (AllCells, CA) were maintained in Iscove's modified Dulbecco's medium containing 5% fetal bovine serum, 2 mM glutamine, 25 mM SaveFull Paper
2008Hanten, JA;Vasilakos, JP;Riter, CL;Neys, L;Lipson, KE;Alkan, SS;Birmachu, W;Comparison of human B cell activation by TLR7 and TLR9 agonistsBMC immunology186526793') was obtained from (Genosys, Woodlands, TX). Isolation and treatment of human peripheral blood B cells Human peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers from AllCells, LLC. (Berkeley, CA) and Memorial Blood CeFull Paper
2008López, MC;Lawrence, DA;Proficiency testing experience for viable CD34+ stem cell analysisTransfusion18298596Human peripheral blood was collected in blood collection tubes (Cyto-Chex BCT, Streck Laboratories, La Vista, NE), which contain a preservative reagent for flow cytometry. Frozen CD34+ stem cells from cord blood (AllCells LLC, Berkeley, CA) were thawFull Paper
2008Tang, H;Lu, JY;Zheng, X;Yang, Y;Reagan, JD;The psoriasis drug monomethylfumarate is a potent nicotinic acid receptor agonistBiochemical and biophysical research communications187223462B were purchased from AllCells (Emeryville, CA) and were of greater than 95% purity. Human CD14+ monocytes and NHEK were from Cambrex (East Rutherford, NJ) and cultured as SaveFull Paper
2008Bachstetter, AD;Pabon, MM;Cole, MJ;Hudson, CE;Sanberg, PR;Willing, AE;Bickford, PC;Gemma, C;Peripheral injection of human umbilical cord blood stimulates neurogenesis in the aged rat brainBMC neuroscience18275610cord blood mononuclear cells (UCBMCs) were obtained from Saneron CCEL Therapeutics, Inc. (Tampa, FL, USA). Cryopreserved Human peripheral blood cells (PBMC) (mononuclear fraction) were obtained from AllCells, LLC (Emeryville, CA, USA). Just prior toFull Paper
2008Mashiyama, ST;Hansen, CM;Roitman, E;Sarmiento, S;Leklem, JE;Shultz, TD;Ames, BN;An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiencyAnalytical biochemistry17963712, respectively, in measuring DNA-uracil in all the samples used in experiments described in this paper (lymphocytes from the B6 deficiency study and lymphocytes from AllCells). Uracil SaveFull Paper
2008Park, EJ;Kim, ES;Weber, HP;Wright, RF;Mooney, DJ;Improved bone healing by angiogenic factor-enriched platelet-rich plasma and its synergistic enhancement by bone morphogenetic protein-2The International journal of oral & maxillofacial implants19014150Fresh human adult whole blood was purchased from AllCells (Emeryville, CA, USA). Peripheral blood mononuclear cells (PBMNCs) and platelets were separately isolated by a density gradient centrifugation (Histopaque-1077, Sigma, St. Louis, MO, USA) accoFull Paper
2008Thamm, DH;O'Brien, MG;Vail, DM;Serum vascular endothelial growth factor concentrations and postsurgical outcome in dogs with osteosarcomaVeterinary and comparative oncology19178672Vascular endothelial growth factor (VEGF) is a key angiogenic growth factor, playing putative roles in both tumour growth and metastasis. The purpose of this study was to correlate SaveFull Paper
2008Antonescu, CR;Wu, K;Xing, GL;Cao, M;Turpaz, Y;Leversha, MA;Hubbell, E;Maki, RG;Miyada, CG;Pillai, R;DNA copy number analysis in gastrointestinal stromal tumors using gene expression microarraysCancer informatics19259404from the American Type Culture Collection (ATCC, Manassas, VA). DNA was extracted from the cultured cells using the DNA Maxi Kit (Qiagen, Inc., Valencia, CA). DNA from normal blood was obtained from AllCells, LLC (Emeryville, CA). GIST sample DNA wasFull Paper
2008Li, M;Rossi, JJ;Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cellsMethods in molecular biology (Clifton, N.J.)18679631Target cells: K562 cells, a chronic myelogenous leukemia cell line (ATCC, #CCL-243). CD34+ hematopoietic stem cells, purchased from AllCells (Berkley, CA, USA) or purified from umbilical cord blood or bone marrow by anti-CD34 antibody-coupled magnetiFull Paper
2008Campbell-Anson, RE;Kentor, D;Wang, YJ;Bushnell, KM;Li, Y;Vence, LM;Radvanyi, LG;A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technologyJournal of immune based therapies and vaccines18321390Sources of PBMC for DC generation PBMC were obtained from peripheral blood leukopheresis products obtained from non-mobilized normal donors (LifeBlood, Memphis, TN), or G-CSF-mobilized normal donors (AllCells, Berkeley, CA). Products were collected iFull Paper
2008Tiainen, J;Structured equity products and portfolio compositions of financially advised investorsThesisRESULTS I will show in this thesis that behavioural biases of the retail investors are associated to proportions of portfolio invested in structured capital guaranteed products and in equity SaveFull Paper
2008Shi, M;The Role of Janus-Kinase-3 in CD4+ T Cell Proliferation and Differentiation: A DissertationThesisAliquots of cryopreserved peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryville, CA). PBMC were resuspended in complete RPMI 1640 medium (RPMI 1640 medium supplemented with 10% fetal bovine serum, penicillin/streptomycin,Full Paper
2008Ziegler, LA;Targeting lentivector gene delivery to hematopoietic stem and progenitor cells in vivoThesisFresh, unfractionated human PBMCs (2 x 106 ) (AllCells) were incubated with concentrated virus with total TUs of 10 x 106 (based on the titer on 293T/CD20 cells). LPS (50 μg/ml) was then added for B cells to survive and grow. After 2 days, cells werFull Paper
2008Wang, M;Genomic-scale transcriptional analysis of T-cell activation reveals novel genes and signaling programsThesisHealthy-donor peripheral blood mononuclear cells (PBMCs) (AllCells, Berkeley, CA) were used to negatively-select CD3+ T cells, CD4+ and CD8+ T cells (Pan T Cell Isolation Kit II, CD4+ T Cell Isolation Kit II, and CD8+ T Cell Isolation Kit II, MiltenyFull Paper
2008Bedi, R;Characterization of the activities of the human C/EBPϵ activator and repressor isoforms in myelopoiesisThesis25 ml/cm2 . After preloading and before prestimulation, the plates were washed with PBS to remove any inhibitory molecules in the viral supernatant. Cord blood CD34+ cells purchased from AllCells (CA, USA) were thawed according to the supplier's instFull Paper
2007Aziz, MH;Manoharan, HT;Church, DR;Dreckschmidt, NE;Zhong, W;Oberley, TD;Wilding, G;Verma, AK;Protein kinase Cepsilon interacts with signal transducers and activators of transcription 3 (Stat3), phosphorylates Stat3Ser727, and regulates its constitutive activation in prostate cancerCancer Res.17875724Prostate cancer specimens were obtained at radical prostatectomy. The University of Wisconsin Human Subjects Committee approved the use of these human prostate specimens. Tissue microarrays were obtained from Folio Biosciences.4 Tissue microarrays weFull Paper
2007Senzer, N;Nemunaitis, J;Nemunaitis, M;Lamont, J;Gore, M;Gabra, H;Eeles, R;Sodha, N;Lynch, FJ;Zumstein, LA;Menander, KB;Sobol, RE;Chada, S;p53 therapy in a patient with Li-Fraumeni syndromeMol. Cancer Ther.17483435Full Paper
2007Gagna, CE;Clark Lambert, W;Cell biology, chemogenomics and chemoproteomics - application to drug discoveryExpert Opin Drug Discov23484648TMAs have been expensive and limited in terms of commercial availability [8,56-59]. Only recently have they surfaced commercially on a large scale (e.g., Cytomyx, Folio Biosciences, Chemicon). Many different types of high quality TMAs now exist, suchFull Paper
2007Flores, J;Cepeda, IL;Cornfeldt, ML;O'Kusky, JR;Doudet, DJ;Characterization and survival of long-term implants of human retinal pigment epithelial cells attached to gelatin microcarriers in a model of Parkinson diseaseJ. Neuropathol. Exp. Neurol.17620984All primary antibodies were previously assessed for hRPE cell specificity in human RPE cultured monolayer and RPE-choroid eye tissue, and for nonspecificity to striatal rat tissue (Dr. S. Bernstein, QualTek Molecular Laboratories, Santa Barbara, CA,Full Paper
2007Lynch, FC;Use of a covered stent for percutaneous transrenal occlusion of the ureterJ Vasc Interv Radiol18004001Full Paper
2007Cantwell, CP;Lynch, FC;Caudal migration of a G2 filter during carbon dioxide cavographyJ Vasc Interv Radiol17538152Full Paper
2007Lee, S;Sha, Q;Wu, X;Calenda, G;Peng, J;Expression profiles of mouse Kell, XK, and XPLAC mRNAJ. Histochem. Cytochem.17189525For the ISH experiments, the custom service from Phylogeny Inc. (Columbus, OH) was used (Lyons et al. 1990; Patapoutian et al. 1993). Briefly, tissue sections were hybridized overnight at 55C in 50% deionized formamide, 0.3 M NaCl, 20 mM Tris-HCl, pHFull Paper
2007Tanimoto, R;Abarzua, F;Sakaguchi, M;Takaishi, M;Nasu, Y;Kumon, H;Huh, NH;REIC/Dkk-3 as a potential gene therapeutic agent against human testicular cancerInt. J. Mol. Med.17273781Tissues, cells and reagents. A testicular carcinoma tissue microarray was purchased from Folio Biosciences (Columbus, OH). Surgically resected testicular cancer tissues were fixed with 4% paraformaldehyde and tissue sections were made under conventioFull Paper
2007Calin, GA;Liu, CG;Ferracin, M;Hyslop, T;Spizzo, R;Sevignani, C;Fabbri, M;Cimmino, A;Lee, EJ;Wojcik, SE;Shimizu, M;Tili, E;Rossi, S;Taccioli, C;Pichiorri, F;Liu, X;Zupo, S;Herlea, V;Gramantieri, L;Lanza, G;Alder, H;Rassenti, L;Volinia, S;Schmittgen, TD;Kipps, TJ;Negrini, M;Croce, CM;Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomasCancer cell17785203An additional set of 3 normal CD19+/CD5+ positive B cells, not used for microarray studies, was purchased from AllCells (Berkeley, CA), and used as an independent confirmation set. In all instances the qRT-PCR data confirmed the microarray data. RNAFull Paper
2007Wang, L;Wang, J;Blaser, BW;Duchemin, AM;Kusewitt, DF;Liu, T;Caligiuri, MA;Briesewitz, R;Pharmacologic inhibition of CDK4/6: mechanistic evidence for selective activity or acquired resistance in acute myeloid leukemiaBlood17537993Entry into the cell cycle is mediated by cyclin-dependent kinase 4/6 (CDK4/6) activation, followed by CDK2 activation. We found that pharmacologic inhibition of the Flt3 internal tandem SaveFull Paper
2007Mulligan, G;Mitsiades, C;Bryant, B;Zhan, F;Chng, WJ;Roels, S;Koenig, E;Fergus, A;Huang, Y;Richardson, P;Trepicchio, WL;Broyl, A;Sonneveld, P;Shaughnessy, JD;Bergsagel, PL;Schenkein, D;Esseltine, DL;Boral, A;Anderson, KC;Gene expression profiling and correlation with outcome in clinical trials of the proteasome inhibitor bortezomibBlood17185464The aims of this study were to assess the feasibility of prospective pharmacogenomics research in multicenter international clinical trials of bortezomib in multiple myeloma and to SaveFull Paper
2007Brogdon, JL;Xu, Y;Szabo, SJ;An, S;Buxton, F;Cohen, D;Huang, Q;Histone deacetylase activities are required for innate immune cell control of Th1 but not Th2 effector cell functionBlood17008546as described previously.22,23 Human neutrophils were obtained from AllCells (Berkeley, CA). Mouse bone marrow-derived DCs were prepared as described previously.24 Murine Th1 and Th2 effector cells were differentiated from DO11.10 mice as describedFull Paper
2007Mahadevan, D;Klinkhammer, T;Cooke, L;Riley, C;Choi, J;Sinari, S;Miller, T;Spier, C;CLL Transcript and Serum Profiling Identifies Distinct Oncogenic Pathways in Rai Stage Progression.BloodFull Paper
2007Robinson, S;Simmons, P;Brouard, N;Kidd, S;Yang, H;Decker, W;Xing, D;Steiner, D;Ng, J;Thomas, M;Champlin, R;de Lima, M;Hosing, C;Cooper, L;Singh, H;Zweidler-McKay, P;Shpall, E;Efficacy of ‘Off-the-Shelf’, Commercially-Available, Third-Party Mesenchymal Stem Cells (MSC) in Ex Vivo Cord Blood (CB) Co-Culture Expansion.BloodINTRODUCTION: Our previous studies have shown that clinically-relevant levels of hematopoietic stem and progenitor cell (HSPC) expansion are possible by ex vivo co-culture of cord blood (CB) mononuclear cells (MNC) with third-party bone marrow (BM)-dFull Paper
2007Majeti, R;Park, CY;Weissman, IL;Identification of a hierarchy of multipotent hematopoietic progenitors in human cord bloodCell stem cell18371405Normal human bone marrow mononuclear cells were purchased from AllCells Inc. (Emeryville, CA). Human cord blood was collected from placentas and/or umbilical cords obtained SaveFull Paper
2007Scholl, C;Bansal, D;Döhner, K;Eiwen, K;Huntly, BJ;Lee, BH;Rücker, FG;Schlenk, RF;Bullinger, L;Döhner, H;Gilliland, DG;Fröhling, S;The homeobox gene CDX2 is aberrantly expressed in most cases of acute myeloid leukemia and promotes leukemogenesisThe Journal of clinical investigation17347684and all patients and volunteers gave informed consent according to the Declaration of Helsinki. Seven of the 10 normal BMMC samples and the 3 normal BM samples that were used for isolating HSCs, CMPs, GMPs, and MEPs were purchased from AllCellsFull Paper
2007Xin, X;Hussain, M;Mao, JJ;Continuing differentiation of human mesenchymal stem cells and induced chondrogenic and osteogenic lineages in electrospun PLGA nanofiber scaffoldBiomaterials17010425Bone-marrow-derived hMSCs were purchased from AllCells (Berkeley, CA, USA). The donor was a 25 year-old healthy male volunteer without additional identifier. In our previous work, we have isolated hMSCs from multiple donors [5]. In this report, the iFull Paper
2007Zou, H;Harrington, JJ;Sugumar, A;Klatt, KK;Smyrk, TC;Ahlquist, DA;Detection of colorectal disease by stool defensin assay: an exploratory studyClinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association17531545These cDNA samples and a purchased neutrophil cNDA (AllCells, Emeryville, CA) were amplified by real-time polymerase chain reaction (PCR) in an iCycler (Bio-Rad, Hercules, CA). SaveFull Paper
2007Zhao, Y;Parkhurst, MR;Zheng, Z;Cohen, CJ;Riley, JP;Gattinoni, L;Restifo, NP;Rosenberg, SA;Morgan, RA;Extrathymic generation of tumor-specific T cells from genetically engineered human hematopoietic stem cells via Notch signalingCancer research17363559CD133 + progenitor cells were enriched from mononuclear cells from UCB (Allcells, Berkeley, CA) by depleting CD3 + cells using Dynabeads human CD3 (Invitrogen, Carlsbad, CA) SaveFull Paper
2007Verhelle, D;Corral, LG;Wong, K;Mueller, JH;Moutouh-de Parseval, L;Jensen-Pergakes, K;Schafer, PH;Chen, R;Glezer, E;Ferguson, GD;Lopez-Girona, A;Muller, GW;Brady, HA;Chan, KW;Lenalidomide and CC-4047 inhibit the proliferation of malignant B cells while expanding normal CD34+ progenitor cellsCancer research17234786Cell culture. Namalwa CSN.70 and LP-1 cells were purchased from DSMZ (Brauschweig, Germany), U266 was purchased from the American Type Culture Collection (Manassas, VA), and cord blood CD34+ cells were purchased from AllCells (Emeryville, CA). NamalwFull Paper
2007Pariente, N;Morizono, K;Virk, MS;Petrigliano, FA;Reiter, RE;Lieberman, JR;Chen, IS;A novel dual-targeted lentiviral vector leads to specific transduction of prostate cancer bone metastases in vivo after systemic administrationMolecular therapy : the journal of the American Society of Gene Therapy17653099endothelial cells were obtained from AllCells (Emeryville, CA) and grown in human umbilical vein endothelial cell basal medium plus stimulatory supplement (AllCells, Emelyville, CA). SaveFull Paper
2007Sonoyama, W;Seo, BM;Yamaza, T;Shi, S;Human Hertwig's epithelial root sheath cells play crucial roles in cementum formationJournal of dental research17586703Human bone marrow mesenchymal stem cells (BMMSCs) were purchased from a commercially available resource (AllCells LLC, Berkeley, CA, USA), and cultured as previously described (Batouli et al., 2003). Cemento/osteogenic differentiation was induced andFull Paper
2007Li, L;Piloto, O;Kim, KT;Ye, Z;Nguyen, HB;Yu, X;Levis, M;Cheng, L;Small, D;FLT3/ITD expression increases expansion, survival and entry into cell cycle of human haematopoietic stem/progenitor cellsBritish journal of haematology17359372Activating mutation of FLT3 by internal tandem duplications (ITDs) in the juxtamembrane region is the most common molecular aberration found in acute myeloid leukaemia (AML). In SaveFull Paper
2007Kikuchi, J;Shimizu, R;Wada, T;Ando, H;Nakamura, M;Ozawa, K;Furukawa, Y;E2F-6 suppresses growth-associated apoptosis of human hematopoietic progenitor cells by counteracting proapoptotic activity of E2F-1Stem cells (Dayton, Ohio)17600109magnetic cell sorting; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com) from human bone marrow mononuclear cells (BM-MNCs) (purchased from AllCells, Berkeley, CA, http://www.allcells.com) or cord blood mononuclear cells (CBFull Paper
2007Telford, WG;Bradford, J;Godfrey, W;Robey, RW;Bates, SE;Side population analysis using a violet-excited cell-permeable DNA binding dyeStem cells (Dayton, Ohio)17185610(FBS)/10% dimethyl sulfoxide in liquid nitrogen until use. Frozen human cord blood was obtained from AllCells LLC (Emeryville, CA, http://www.allcells.com) and thawed immediately before use. A549 lung carcinoma cells were grown in Dulbecco's modifiedFull Paper
2007Fang, D;Seo, BM;Liu, Y;Sonoyama, W;Yamaza, T;Zhang, C;Wang, S;Shi, S;Transplantation of mesenchymal stem cells is an optimal approach for plastic surgeryStem cells (Dayton, Ohio)17170063Human bone marrow cells were purchased from commercially available resources (AllCells LLC, Berkeley, CA,http://www.allcells.com/). To identify putative bone marrow MSCs, single-cell suspension of 1 × 106 of bone marrow mononuclear cells were seededFull Paper
2007Stosich, MS;Mao, JJ;Adipose tissue engineering from human adult stem cells: clinical implications in plastic and reconstructive surgeryPlastic and reconstructive surgery17255658Human mesenchymal stem cells were isolated from fresh bone marrow samples of an anonymous adult male donor (AllCells, Berkeley, Calif.) by following our previous methods. After SaveFull Paper
2007Kim, JS;Lee, C;Bonifant, CL;Ressom, H;Waldman, T;Activation of p53-dependent growth suppression in human cells by mutations in PTEN or PIK3CAMolecular and cellular biology17060456were described in a previous study (27). BJ-hTERT and RPE-hTERT cells were obtained from Clontech (Mountain View, CA). Normal human astrocytes (NHA) were obtained from AllCells LLC (Emeryville, CA) and cultured as recommended. HEK293, MCF10A, and A17Full Paper
2007Chen, C;Fuhrken, PG;Huang, LT;Apostolidis, P;Wang, M;Paredes, CJ;Miller, WM;Papoutsakis, ET;A systems-biology analysis of isogenic megakaryocytic and granulocytic cultures identifies new molecular components of megakaryocytic apoptosisBMC genomics17953764tudies of individual candidate genes. Methods Hematopoietic cultures Mk and G cell cultures were initiated with fresh or frozen G-CSF-mobilized human peripheral blood CD34+ cells from normal donors (AllCells; Berkeley, CA) and cultured as describedFull Paper
2007Vose, SC;Holland, NT;Eskenazi, B;Casida, JE;Lysophosphatidylcholine hydrolases of human erythrocytes, lymphocytes, and brain: sensitive targets of conserved specificity for organophosphorus delayed neurotoxicantsToxicology and applied pharmacology17663017Whole human blood from seven subjects and lymphocytes (isolated by aphaeresis using a leukopac filter) from three subjects were obtained from AllCells LLC (Emeryville, CA). Mouse SaveFull Paper
2007Zeng, Z;Yuan, X;Liu, G;Zeng, X;Zeng, X;Ng, H;Chen, H;Jiang, T;Akasaki, Y;Kessey, K;Black, KL;Yu, JS;Manipulation of proliferation and differentiation of human bone marrow-derived neural stem cells in vitro and in vivoJournal of neuroscience research17131390Human bone marrow cells (HBMC) were isolated from fresh human whole bone marrow (AllCells, Berkely, CA) by gradient density centrifugation (Lee et al., 2003). HBMC were plated at a density of 2 × 106 cells/ml/well onto 24-well plates (Corning, CorninFull Paper
2007Huang, LT;Paredes, CJ;Papoutsakis, ET;Miller, WM;Gene expression analysis illuminates the transcriptional programs underlying the functional activity of ex vivo-expanded granulocytesPhysiological genomics17550995of PB granulocytes should reflect their full activation potential, because RNA levels would not be expected to change very much during cell transportation and processing by AllCells (Full Paper
2007Uno, S;Kinoshita, Y;Azuma, Y;Tsunenari, T;Yoshimura, Y;Iida, S;Kikuchi, Y;Yamada-Okabe, H;Fukushima, N;Antitumor activity of a monoclonal antibody against CD47 in xenograft models of human leukemiaOncology reports17390064The ligation of CD47 induces the apoptosis of leukemic cells in a caspase-independent manner. We generated a monoclonal antibody against CD47 (mAb-MABL) that possibly induced SaveFull Paper
2007Jenkins, J;Calzone, KA;Establishing the essential nursing competencies for genetics and genomicsJournal of nursing scholarship : an official publication of Sigma Theta Tau International Honor Society of Nursing17393960129This annotation involves adding the units and labels to allcells containing no references to other cells. The systemthen analyzes the cells with formulas containing referencesand SaveFull Paper
2007Furuhata, S;Ando, K;Oki, M;Aoki, K;Ohnishi, S;Aoyagi, K;Sasaki, H;Sakamoto, H;Yoshida, T;Ohnami, S;Gene expression profiles of endothelial progenitor cells by oligonucleotide microarray analysisMolecular and cellular biochemistry17203242Among the many tissue stem or progenitor cells recently being unveiled, endothelial progenitor cells (EPCs) have attracted particular attention, not only because of their cardinal role in SaveFull Paper
2007Yourek, G;Hussain, MA;Mao, JJ;Cytoskeletal changes of mesenchymal stem cells during differentiationASAIO journal (American Society for Artificial Internal Organs : 1992)17413564Human mesenchymal stem cells were isolated from commercially available whole bone marrow (AllCells, Berkeley, CA) using RosetteSep (StemCell Technologies, Vancouver, BC) according to the manufacturer’s instructions. The RosetteSep antibody cocktail cFull Paper
2007Baysal, BE;A recurrent stop-codon mutation in succinate dehydrogenase subunit B gene in normal peripheral blood and childhood T-cell acute leukemiaPloS one1748727580°C. cDNAs synthesized from column-purified normal PBMC cell-type subsets, including monocytes (CD14+), NK cells (CD56+, negative selection), B (CD19+) and T lymphocytes (CD3+), were purchased from AllCells, LLC Inc. through StemCell Technologies. EFull Paper
2007Stosich, MS;Bastian, B;Marion, NW;Clark, PA;Reilly, G;Mao, JJ;Vascularized adipose tissue grafts from human mesenchymal stem cells with bioactive cues and microchannel conduitsTissue engineering17824832Human MSCs were isolated from fresh bone marrow samples of two anonymous adult donors (AllCells, Berkeley, CA), per our previous methods.After transferring bone marrow sample SaveFull Paper
2007Popov, BV;Serikov, VB;Petrov, NS;Izusova, TV;Gupta, N;Matthay, MA;Lung epithelial cells induce endodermal differentiation in mouse mesenchymal bone marrow stem cells by paracrine mechanismTissue engineering17630877Human hematopoietic stem cells (CD34þ) were purchased from AllCells Co. (Berkeley, CA) and confirmed to be 96% pure using FACScan. Cells were washed twice in PBS, labeled with PKH26 (Sigma) according to manufacturer’s instructions, and mixed with epiFull Paper
2007Moioli, EK;Hong, L;Mao, JJ;Inhibition of osteogenic differentiation of human mesenchymal stem cellsWound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society17537129hMSCs were isolated from donated human bone marrow samples (AllCells, Berkeley, CA) and plated in monolayers in six-well plates at a density of 3×104 cells/well per our previous methods36 (Figure 1A and B). The isolated hMSCs were cultured-expanded aFull Paper
2007Anderson, RM;Stevens, DL;Sumption, ND;Townsend, KM;Goodhead, DT;Hill, MA;Effect of linear energy transfer (LET) on the complexity of alpha-particle-induced chromosome aberrations in human CD34+ cellsRadiation research17474795Frozen human bone marrow CD34 + cells were obtained commercially (Poietics, Cambrex and AllCells, LLC) in vials of 1 × 10 6 cells (samples are anonymized and exempt from SaveFull Paper
2007Sawyer, L;Hanson, D;Castro, G;Luckett, W;Dubensky, TW;Stassinopoulos, A;Inactivation of parvovirus B19 in human platelet concentrates by treatment with amotosalen and ultraviolet A illuminationTransfusion17524098CD34+ cells isolated from granulocyte-colony-stimulating factor-mobilized human peripheral blood were obtained from AllCells (Berkeley, CA) and stimulated to proliferate and SaveFull Paper
2007Wang, TT;Tio, M;Lee, W;Beerheide, W;Udolph, G;Neural differentiation of mesenchymal-like stem cells from cord blood is mediated by PKABiochemical and biophysical research communications17466951Cell culture and growth profiling. MNCs (AllCells) were seeded in MyeloCult medium (Stem Cell Technologies) supplemented with 1 × 10−7 M dexamethasone (Sigma). Adherent cells were trypsinized (0.125% Trypsin-EDTA) and passaged at near-confluency (70-Full Paper
2007Chen, L;Gao, Z;Zhu, J;Rodgers, GP;Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrowExperimental hematology17588473Human bone marrow CD133+ cells (AllCells, Berkeley, CA, USA) were cultured for 14-28 days in the presence of EPO or G-CSF. All cells were grown in media that included stem cell factor (SCF, 50 ng/mL), interleukin-3 (IL-3, 10 ng/mL), granulocyte-macroFull Paper
2007Chua, KN;Chai, C;Lee, PC;Ramakrishna, S;Leong, KW;Mao, HQ;Functional nanofiber scaffolds with different spacers modulate adhesion and expansion of cryopreserved umbilical cord blood hematopoietic stem/progenitor cellsExperimental hematology17577926Frozen human umbilical cord blood CD34+ HSPCs were purchased from AllCells, LLC (Emeryville, CA, USA). The CD34+ purity in the post-thawed HSPC was determined to be 98% by flow cytometry and the viability was determined to be more than 97% by TrypanFull Paper
2007Zwezdaryk, KJ;Coffelt, SB;Figueroa, YG;Liu, J;Phinney, DG;LaMarca, HL;Florez, L;Morris, CB;Hoyle, GW;Scandurro, AB;Erythropoietin, a hypoxia-regulated factor, elicits a pro-angiogenic program in human mesenchymal stem cellsExperimental hematology17379074Cambrex BioScience (Walkersville, MD, USA) and Allcells (Emeryville, CA, USA), to ensure enough variability of the starting cell population and make certain that findings are universal and not unique to single donor pools derived from a unique sourceFull Paper
2007Cheng, X;Macvittie, T;Meisenberg, B;Welty, E;Farese, A;Tadaki, D;Takebe, N;Human brain endothelial cells (HUBEC) promote SCID repopulating cell expansion through direct contactGrowth factors (Chur, Switzerland)18049950The objective of this study was to re-evaluate the previously published hematopoietic stem cell (HSC) expansion work using human brain endothelial cells (HUBEC). The expansion SaveFull Paper
2007Basiji, DA;Ortyn, WE;Liang, L;Venkatachalam, V;Morrissey, P;Cellular image analysis and imaging by flow cytometryClinics in laboratory medicine17658411To investigate the utility of imaging flow cytometry for clinical FISH analysis, human peripheral blood mononuclear cells (PBMC) were obtained (AllCells, San Diego, CA) and probed using a FISH in suspension (FISHIS®) protocol developed at Amnis CorpoFull Paper
2007King, JA;Cell culture systems inspired by the hematopoietic stem cell nicheThesiswere maintained between 0.5 and 1x106 cells/mL. 3.3.5 Culture of CD34+ selected cells CD34+ selected cells were purchased from AllCells (Berkeley, CA). AllCells obtained informed consent from volunteers before donors provided cellsFull Paper
2007Bell, A;Meyer-Ebrecht, D;Bocking, A;Aach, T;HDR-Microscopy of Cell Specimens: Imaging and Image Analysis2007 Conference Record of the Forty-First Asilomar Conference on Signals, Systems and ComputersThe counterstain affects allcells, ie, independent of the protein which is stained by the immunocytochemical marker. Hence, marker positive cells will be affected by both stains at once. SaveFull Paper
2007이연경, ;Effects of cationic liposomes on in vitro and in vivo transduction mediated by adenoviral vectorsThesisAccording to theresultofGFP expression in HeLa cells(Figure 3A),nearly allcells were transduced in the presence of the 100 MOIof Ad-vectors. Meanwhile, CAR-deficient B16BL6 cells SaveFull Paper
2007NGIAP, CK;Bio-functionalization of Electrospun Nanofibre Scaffolds for Cell Culture ApplicationsThesisFrozen human umbilical cord blood CD34+ HSPCs were purchased from AllCells, which were obtained from normal volunteers participating in an Institute Reviewing Board (IRB) approved donor program (AllCells). The CD34+ purity in the HSPC was determineFull Paper
2007Giammona, LM;Nicotinamide enhances primary human megakaryocytic differentiation from hematopoietic stem cells: Phenotypic characterization and mechanism of actionThesisUnless otherwise noted, all reagents were obtained from Sigma-Aldrich (St. Louis, MO). Cultures were initiated with previously frozen human mobilized peripheral blood CD34+ cells (AllCells; Berkeley, CA). Cells were cultured in T-flasks and maintaineFull Paper
2007Troken, AJ;Mesenchymal stem cells and derivatives in hydrogelsThesisHMSCs were isolated from commercially available healthy human bone marrow (AllCells, LLC; Berkeley, CA) using RosetteSep kit (StemCell Technologies, Inc., Vancouver, BC, Canada). Bone marrow extracts contain heterogeneous cell populations. MSCs repreFull Paper
2006Perk, J;Gil-Bazo, I;Chin, Y;de Candia, P;Chen, JJ;Zhao, Y;Chao, S;Cheong, W;Ke, Y;Al-Ahmadie, H;Gerald, WL;Brogi, E;Benezra, R;Reassessment of id1 protein expression in human mammary, prostate, and bladder cancers using a monospecific rabbit monoclonal anti-id1 antibodyCancer Res.17108123Two slides of commercially available (Folio Biosciences, Columbus, OH) TMAs were also analyzed. The two slides included a total of 143 cores, of which 62 samples were common types of breast cancer, and the rest of the samples were benign (slides #ARYFull Paper
2006Swales, KE;Korbonits, M;Carpenter, R;Walsh, DT;Warner, TD;Bishop-Bailey, D;The farnesoid X receptor is expressed in breast cancer and regulates apoptosis and aromatase expressionCancer Res.17047076Samples of human infiltrating ductal breast carcinoma and near tumor normal samples were obtained from 10 patients after receiving ethical approval and individual patient consent. Protein was extracted using Cytobuster (Novagen, Merck Biosciences, NoFull Paper
2006Evans, EE;Henn, AD;Jonason, A;Paris, MJ;Schiffhauer, LM;Borrello, MA;Smith, ES;Sahasrabudhe, DM;Zauderer, M;C35 (C17orf37) is a novel tumor biomarker abundantly expressed in breast cancerMol. Cancer Ther.17121940Staining of formalin-fixed, paraffin-embedded breast carcinoma and normal tissue sections was done in part by QualTek Molecular Labs (Santa Barbara, CA). Ten lobular and 50 invasive ductal (grades 1, 2, and 3) carcinomas were selected from the QualTeFull Paper
2006Roth, JD;Hughes, H;Kendall, E;Baron, AD;Anderson, CM;Antiobesity effects of the beta-cell hormone amylin in diet-induced obese rats: effects on food intake, body weight, composition, energy expenditure, and gene expressionEndocrinology16935845To compare central nervous system gene expression across amylin- and pair-fed-treated rats that had exhibited a similar magnitude of weight loss, four animals from each group were selected for ISH, counterbalanced for their final body weight on the dFull Paper
2006Couse, JF;Yates, MM;Rodriguez, KF;Johnson, JA;Poirier, D;Korach, KS;The intraovarian actions of estrogen receptor-alpha are necessary to repress the formation of morphological and functional Leydig-like cells in the female gonadEndocrinology16627580... }X56974 [/nuccore/X56974] 1271–1351 GAAACTGCGAATGGCTCATTAA GAATTACCACAGTTATCCAAGTAGGA Open in a separate window [/pmc/articles/PMC1892221/table/T1/?report=objectonly] IN SITU HYBRIDIZATION Phylogeny Inc. (Columbus, OH) conducted all _inFull Paper
2006Costello, C;Ward, M;Search, bioprospecting and biodiversity conservationJournal of Environmental Economics and Managementin a particular way. When executed in bulk, these tests cost between $4000 and $18,000 per species tested (eg Qualtek Molecular Laboratories, Santa Barbara, CA). We use a range for c of $4000-$18,000. • Discount rate (rFull Paper
2006Cantwell, CP;Lynch, FC;Ureterocutaneous fistula and urostomy exclusion with use of a covered wallstentJ Vasc Interv Radiol16614159Full Paper
2006Kutty, RK;Chen, S;Samuel, W;Vijayasarathy, C;Duncan, T;Tsai, JY;Fariss, RN;Carper, D;Jaworski, C;Wiggert, B;Cell density-dependent nuclear/cytoplasmic localization of NORPEG (RAI14) proteinBiochem. Biophys. Res. Commun.16729964Immunohistochemistry. Immunohistochemistry was performed by QualTek Molecular Laboratories. Briefly, four-micron human placenta sections were deparaffinized, subjected to 20 min of steam heat induced epitope recovery, and then treated with anti-NORPEFull Paper
2006Cantwell, C;Waybill, P;Lynch, F;Taubman, K;Inferior vena cava duplication and deep venous thrombosisAnn Vasc Surg16575497Full Paper
2006Huang, LT;Transcriptional analysis of ex vivo granulocyte (neutrophil) developmentThesisCultures were initiated with fresh or frozen G-CSF mobilized peripheral blood CD34+ cells from normal donors (AllCells, Emeryville, CA), at a seeding density of 3 x 104 cells/mL. Cells were cultured in human long-term medium (HLTM) at a pH of 7.1 anFull Paper
2006Chen, C;Huang, X;Atakilit, A;Zhu, QS;Corey, SJ;Sheppard, D;The Integrin alpha9beta1 contributes to granulopoiesis by enhancing granulocyte colony-stimulating factor receptor signalingImmunity17137800Murine bone marrow or peripheral blood cells were collected at autopsy and suspended in phosphate-buffered saline (PBS). Human bone marrow cells were obtained from Allcells (Berkeley, CA). Cells were incubated with primary antibody for 30 min at 4°C,Full Paper
2006Guerzoni, C;Bardini, M;Mariani, SA;Ferrari-Amorotti, G;Neviani, P;Panno, ML;Zhang, Y;Martinez, R;Perrotti, D;Calabretta, B;Inducible activation of CEBPB, a gene negatively regulated by BCR/ABL, inhibits proliferation and promotes differentiation of BCR/ABL-expressing cellsBlood16418324CD34+ normal bone marrow (NBM) cells from different healthy donors were purchased from AllCells (LLC, Berkley, CA) and their use was approved by the Ohio State University Institutional Review Board (IRB). Mononuclear hematopoietic cells from bone marFull Paper
2006Notari, M;Neviani, P;Santhanam, R;Blaser, BW;Chang, JS;Galietta, A;Willis, AE;Roy, DC;Caligiuri, MA;Marcucci, G;Perrotti, D;A MAPK/HNRPK pathway controls BCR/ABL oncogenic potential by regulating MYC mRNA translationBlood16293596CD34+ normal bone marrow (NBM) cells from healthy donors were from AllCells (Berkeley, CA). Studies performed with human specimens from The Ohio State University Leukemia Tissue Bank (Columbus, OH), the Division of Hematology, Maisonneuve-Rosemont HoFull Paper
2006Choi, J;Garewal, H;Riley, C;Cooke, L;Klinkhammer, T;Maddipati, S;Sud, R;Spier, C;Mahadevan, D;Transcriptosome and Serum Cytokine Profiling of B-CLL Identifies Oncogenic Pathways in Pathogenesis and Survival.BloodFull Paper
2006Greaney, P;Nahimana, A;Lagopoulos, L;Etter, AL;Aubry, D;Attinger, A;Beltraminelli, N;Huni, B;Bassi, I;Sordat, B;Demotz, S;Dupuis, M;Duchosal, MA;A Fas agonist induces high levels of apoptosis in haematological malignanciesLeukemia research16181674CLL 1, 2 and 3, κ+). These cells were frozen in medium containing 10% DMSO within 10 h after harvesting. Haematopoietic progenitor cells from cord blood were obtained frozen from AllCells (San Mateo, CA)Full Paper
2006Chua, KN;Chai, C;Lee, PC;Tang, YN;Ramakrishna, S;Leong, KW;Mao, HQ;Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cellsBiomaterials16854459Interaction between hematopoietic stem/progenitor cells (HSPCs) and their extra cellular matrix components is an integral part of the signaling control for HSPC survival, proliferation SaveFull Paper
2006La Rosée, P;Jia, T;Demehri, S;Härtel, N;de Vries, P;Bonham, L;Hollenback, D;Singer, JW;Melo, JV;Druker, BJ;Deininger, MW;Antileukemic activity of lysophosphatidic acid acyltransferase-beta inhibitor CT32228 in chronic myelogenous leukemia sensitive and resistant to imatinibClinical cancer research : an official journal of the American Association for Cancer Research17085669without erythropoietin [for culture of granulocyte-macrophage colony-forming unit (CFU-GM)] were done as described previously (26). Normal bone marrow was purchased commercially (AllCells, LLC, Berkeley, CA). Patient samples were collected from patieFull Paper
2006Yang, L;Bailey, L;Baltimore, D;Wang, P;Targeting lentiviral vectors to specific cell types in vivoProceedings of the National Academy of Sciences of the United States of America16864770106) (AllCells) were incubated with concentrated virus with total TUs of 10 106 fresh human PBMCs (AllCells) were transferred by tail vein injection into each mouse. After 6 h, SaveFull Paper
2006Giammona, LM;Fuhrken, PG;Papoutsakis, ET;Miller, WM;Nicotinamide (vitamin B3) increases the polyploidisation and proplatelet formation of cultured primary human megakaryocytesBritish journal of haematology17054670Unless otherwise noted, all reagents were obtained from Sigma-Aldrich (St Louis, MO, USA). Cultures were initiated with previously frozen human mobilised peripheral blood (mPB) CD34+ cells (AllCells, Berkeley, CA, USA). Cells were cultured in T-flaskFull Paper
2006Wang, HJ;Yu, CL;Kishi, H;Motoki, K;Mao, ZB;Muraguchi, A;Suppression of experimental osteoarthritis by adenovirus-mediated double gene transferChinese medical journal16934183Allcells were grown to approxim ately 60% 一 70% confluence at the third passage before adenoviraI vector transduction experim ents started.The cells were washed with sterile saline SaveFull Paper
2006Cabana, R;Frolova, EG;Kapoor, V;Thomas, RA;Krishan, A;Telford, WG;The minimal instrumentation requirements for Hoechst side population analysis: stem cell analysis on low-cost flow cytometry platformsStem cells (Dayton, Ohio)16888279Institute Laboratory Animal Facility. Mice were euthanized by gradual CO2 exposure according to NIH guidelines. Bone marrow was extracted from femurs and tibias and used with no further fractionation. Frozen human bone marrow and cord blood were obtaFull Paper
2006Yu, X;Alder, JK;Chun, JH;Friedman, AD;Heimfeld, S;Cheng, L;Civin, CI;HES1 inhibits cycling of hematopoietic progenitor cells via DNA bindingStem cells (Dayton, Ohio)16513761Normal human placental/umbilical cord blood (CB) CD34+ cells were purchased from AllCells (Berkeley, CA, http://www.allcells.com). Normal BM and granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood stem cell (PBSC) CD34+ cells werFull Paper
2006McIntosh, K;Zvonic, S;Garrett, S;Mitchell, JB;Floyd, ZE;Hammill, L;Kloster, A;Di Halvorsen, Y;Ting, JP;Storms, RW;Goh, B;Kilroy, G;Wu, X;Gimble, JM;The immunogenicity of human adipose-derived cells: temporal changes in vitroStem cells (Dayton, Ohio)16410391Human bone marrow was purchased from Cambrex (Walkersville, MD, http://www.cambrex.com) or AllCells, LLC (Berkeley, CA). Bone marrow aspirates were collected with heparin and fractionated over a 1.073 g/ml density gradient (Lymphocyte Separation MediFull Paper
2006Miura, M;Miura, Y;Padilla-Nash, HM;Molinolo, AA;Fu, B;Patel, V;Seo, BM;Sonoyama, W;Zheng, JJ;Baker, CC;Chen, W;Ried, T;Shi, S;Accumulated chromosomal instability in murine bone marrow mesenchymal stem cells leads to malignant transformationStem cells (Dayton, Ohio)16282438Human bone marrow aspirates from healthy adult volunteers were purchased from AllCells, LLC (Berkeley, CA, http://www.allcells.com). To identify putative BMMSCs, single-cell suspension of 1 × 106 of bone marrow mononuclear cells was seeded into 15-cmFull Paper
2006Muramoto, GG;Chen, B;Cui, X;Chao, NJ;Chute, JP;Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injuryBiology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation16635788Cryopreserved human BM CD34+ (Cambrex, Gaithersburg, MD) or CB CD34+ cells (AllCells, Berkeley, CA) were thawed, washed once, and resuspended at 1 × 106/mL in Iscove modified Dulbecco medium (IMDM; Invitrogen) containing 10% FBS and 1% penicillin/strFull Paper
2006Wang, J;Wu, X;Simonavicius, N;Tian, H;Ling, L;Medium-chain fatty acids as ligands for orphan G protein-coupled receptor GPR84The Journal of biological chemistry16966319Quantitative Reverse Transcriptase-mediated PCR (Q-RT-PCR) Analysis—Total RNA from human or mouse tissues (Clontech) and human immune cells (AllCells, LLC) were treated with DNase I (Ambion) before reverse transcription. Q-RT-PCR was performed on anFull Paper
2006Wang, J;Simonavicius, N;Wu, X;Swaminath, G;Reagan, J;Tian, H;Ling, L;Kynurenic acid as a ligand for orphan G protein-coupled receptor GPR35The Journal of biological chemistry16754668Cytokine Secretion—Human peripheral blood mononuclear cells and CD14 + monocytes were purchased from Allcells. Peripheral blood mononuclear cells were seeded at a density of SaveFull Paper
2006Slukvin, II;Vodyanik, MA;Thomson, JA;Gumenyuk, ME;Choi, KD;Directed differentiation of human embryonic stem cells into functional dendritic cells through the myeloid pathwayJournal of immunology (Baltimore, Md. : 1950)16493050Adult PBMC were purchased from AllCells. Monocytes were isolated by adherence to plastic and were cultured for 6 days in RPMI 1640 (Invitrogen Life Technologies) containing 1% FBS (HyClone) in the presence of 100 ng/ml GM-CSF and 100 ng/ml IL-4 withFull Paper
2006Zhang, W;Hirschler-Laszkiewicz, I;Tong, Q;Conrad, K;Sun, SC;Penn, L;Barber, DL;Stahl, R;Carey, DJ;Cheung, JY;Miller, BA;TRPM2 is an ion channel that modulates hematopoietic cell death through activation of caspases and PARP cleavageAmerican journal of physiology. Cell physiology16306129RNA from CD34 + cells and fresh monocytes was purchased from AllCells (Foster City, CA). cDNA was prepared from RNA with the Superscript first-strand synthesis system (Invitrogen, SaveFull Paper
2006Geddis, AE;Kaushansky, K;Endomitotic megakaryocytes form a midzone in anaphase but have a deficiency in cleavage furrow formationCell cycle (Georgetown, Tex.)16552179albumin gradient. For human MKs, CD34+ cells were purchased from AllCells (Berkley, CA) and thawed according to manufacturer’s instructions. The cells were then differentiated by culture in StemPro (Invitrogen) containing 100 ng/ml human TPO, 10 ng/mFull Paper
2006Shen, Y;Chow, J;Wang, Z;Fan, G;Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cellsHuman molecular genetics16870691Medium was changed everyday for optimal cell growth. Fetal astrocytes were purchased from AllCells LLC., and cultures were expanded as suggested by the vendor. Fetal brain tissue was obtained from the Surgical Pathology Laboratory at UCLA (Dr. MichaeFull Paper
2006Polson, AG;Zheng, B;Elkins, K;Chang, W;Du, C;Dowd, P;Yen, L;Tan, C;Hongo, JA;Koeppen, H;Ebens, A;Expression pattern of the human FcRH/IRTA receptors in normal tissue and in B-chronic lymphocytic leukemiaInternational immunology16849395Human blood samples were collected from healthy individuals through the Genentech research blood program; bone marrow was obtained from AllCells (Berkeley, CA, USA) and SaveFull Paper
2006Zhang, X;Kim, J;Ruthazer, R;McDevitt, MA;Wazer, DE;Paulson, KE;Yee, AS;The HBP1 transcriptional repressor participates in RAS-induced premature senescenceMolecular and cellular biology16966377Oncogene-mediated premature senescence has emerged as a potential tumor-suppressive mechanism in early cancer transitions. Previous work shows that RAS and p38 MAPK SaveFull Paper
2006Feng, Q;Chai, C;Jiang, XS;Leong, KW;Mao, HQ;Expansion of engrafting human hematopoietic stem/progenitor cells in three-dimensional scaffolds with surface-immobilized fibronectinJournal of biomedical materials research. Part A16739181Frozen human CB CD34+ cells were purchased from All-Cells (San Mateo, CA), which were obtained from normal volunteers participating in an Institute Reviewing Board (IRB) approved donor program (AllCells). Cells (92% CD34+, 95% viable) were thawed accFull Paper
2006Weigel-Kelley, KA;Yoder, MC;Chen, L;Srivastava, A;Role of integrin cross-regulation in parvovirus B19 targetingHuman gene therapy16972759Primary human CD71 erythroid progenitor cells were purchased from AllCells (Berkeley, CA) and received in Iscove's modified Dulbecco's medium (IMDM)- 0.5% bovine serum SaveFull Paper
2006Braselmann, S;Taylor, V;Zhao, H;Wang, S;Sylvain, C;Baluom, M;Qu, K;Herlaar, E;Lau, A;Young, C;Wong, BR;Lovell, S;Sun, T;Park, G;Argade, A;Jurcevic, S;Pine, P;Singh, R;Grossbard, EB;Payan, DG;Masuda, ES;R406, an orally available spleen tyrosine kinase inhibitor blocks fc receptor signaling and reduces immune complex-mediated inflammationThe Journal of pharmacology and experimental therapeutics16946104Stimulation of Macrophages Derived from Primary Monocytes with IgG or LPS. Human primary macrophages were derived from CD14+ peripheral blood mononuclear cell (AllCells, LLC, Emeryville, CA) according to the protocol specified in the monocyte isolatiFull Paper
2006Xu, H;Othman, SF;Hong, L;Peptan, IA;Magin, RL;Magnetic resonance microscopy for monitoring osteogenesis in tissue-engineered construct in vitroPhysics in medicine and biology16424591Mesenchymal stem cells (MSCs) isolated from fresh adult human bone marrow were provided commercially by AllCells (AllCells LLC, Berkeley, CA). Nucleated cells were incubated in SaveFull Paper
2006Troken, A;Marion, N;Mao, J;HETEROTYPIC ADIPOGENIC PHENOTYPE WHEN EMPLOYING CHONDROGENIC TREATMENT OF HUMAN MESENCHYMAL STEM CELLSASAIO JournalDonated hMSCs (AllCells, LLC) were isolated and expanded using basal media. Upon 90% confluence, the cultures were plated at a density of 24,000 cells/cm 2 in 6-well plates. Three SaveFull Paper
2006Yamada, H;Kunisato, A;Kawahara, M;Tahimic, CGT;Ren, X;Ueda, H;Nagamune, T;Katoh, M;Inoue, T;Nishikawa, M;Oshimura, M;Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosomeJournal of human genetics16333524Both EGFP-21ΔqHAC vectors and ScFvg-21ΔqHAC vectors retained in CHO cells were transferred to 1×107 hBM MNCs (AllCells, LLC, Berkeley, CA, USA) or 1×106 hCB CD34+ cells by microcell-mediated chromosome transfer (MMCT) as previously described (DohertyFull Paper
2006Wang, T;Blatt, LM;Seiwert, SD;Immunomodulatory activities of IFN-gamma1b in combination with type I IFN: implications for the use of IFN-gamma1b in the treatment of chronic HCV infectionsJournal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research16800786IL-4 for 2 weeks), and immature pDCs (IFN-producing cells [IPC], defined as CD11C, CD123, and Lin) were purchased from AllCells, LLC (Berkeley, CA). The procedures for their collection were approved by an Institute Review Board and complied with locaFull Paper
2006Duramad, P;Tager, IB;Leikauf, J;Eskenazi, B;Holland, NT;Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1Journal of applied toxicology : JAT16871525emale) who were sampled repeatedly, for experiments1-13, or were purchased (n=8) from AllCells (Berkeley,CA) for experiments 14 -19. Specimens were collecteddirectly into vacutainers with heparin and processedwithin 2Full Paper
2006Aslan, H;Zilberman, Y;Arbeli, V;Sheyn, D;Matan, Y;Liebergall, M;Li, JZ;Helm, GA;Gazit, D;Gazit, Z;Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regenerationTissue engineering16674300Human mesenchymal stem cells (hMSCs) were isolated from the bone marrow of an anonymous healthy donor (AllCells, Berkeley, CA), cultureexpanded and seeded in 6-well plates at a density of 30,000 cells/well. ForFull Paper
2006Peptan, IA;Hong, L;Xu, H;Magin, RL;MR assessment of osteogenic differentiation in tissue-engineered constructsTissue engineering16674297Human bone marrow cells provided commercially(AllCells Limited Liability Company, Berkeley, CA)were plated at a density of 107nucleated cells per 100mm petri dish and incubated in a culture medium com-posed of Dulbecco’s modifiedFull Paper
2006Stroke, IL;Cole, AG;Simhadri, S;Brescia, MR;Desai, M;Zhang, JJ;Merritt, JR;Appell, KC;Henderson, I;Webb, ML;Identification of CXCR3 receptor agonists in combinatorial small-molecule librariesBiochemical and biophysical research communications16930533Human peripheral blood mononuclear cells (AllCells, Berkeley, CA) were cultured for 3 days with 10 μg/mL phytohemagglutinin, then for 4-7 days with 100 U/mL interleukin-2 ( SaveFull Paper
2006Sugo, T;Tachimoto, H;Chikatsu, T;Murakami, Y;Kikukawa, Y;Sato, S;Kikuchi, K;Nagi, T;Harada, M;Ogi, K;Ebisawa, M;Mori, M;Identification of a lysophosphatidylserine receptor on mast cellsBiochemical and biophysical research communications16460680The total RNAs from human basophils and eosinophils were purchased from AllCells Limited Liability Company (Berkeley, CA). Total RNA was reverse transcribed using Superscript SaveFull Paper
2006Ong, HT;Timm, MM;Greipp, PR;Witzig, TE;Dispenzieri, A;Russell, SJ;Peng, KW;Oncolytic measles virus targets high CD46 expression on multiple myeloma cellsExperimental hematology16728275Fresh bone marrow aspirate from a healthy donor was purchased from AllCells (Berkeley, CA, USA). The mononuclear cell fraction was isolated by Ficoll-Hypaque gradient and used in SaveFull Paper
2006Yoshikubo, T;Inoue, T;Noguchi, M;Okabe, H;Differentiation and maintenance of mast cells from CD34(+) human cord blood cellsExperimental hematology16543066Frozen human umbilical cord blood CD34+ cells were purchased from AllCells, LLC (Berkeley, CA, USA). The mouse bone marrow-derived stroma cell line MS-5 was kindly provided by K. Itoh [23]. MS-5 cells were maintained in a minimal essential medium α (Full Paper
2006Hong, L;Peptan, IA;Xu, H;Magin, RL;Nondestructive evaluation of osteogenic differentiation in tissue-engineered constructsJournal of orthopaedic research : official publication of the Orthopaedic Research Society16583444Healthy fresh human bone marrow was provided commercially (AllCells Limited Liability Company, Emeryville, CA) and plated at a density of 107 nucleated cells per 100 mm petri dish. The cells were incubated in a basic medium of Dulbecco’s Modified EagFull Paper
2006Cole, AG;Stroke, IL;Brescia, MR;Simhadri, S;Zhang, JJ;Hussain, Z;Snider, M;Haskell, C;Ribeiro, S;Appell, KC;Henderson, I;Webb, ML;Identification and initial evaluation of 4-N-aryl-[1,4]diazepane ureas as potent CXCR3 antagonistsBioorganic & medicinal chemistry letters16213722一般社団法人情 処理学会では複写複製および転載複製に係る著作権を学術著作権協会に委託 しています. 当該利用をご希望の方は, 学術著作権協会 が提供している複製利用許諾システ SaveFull Paper
2006Liu, WM;Li, R;Sun, JZ;Wang, J;Tsai, J;Wen, W;Kohlmann, A;Williams, PM;PQN and DQN: algorithms for expression microarraysJournal of theoretical biology16889801Here, we disclose two triplets of experiments using 3 ml fresh bone marrow from a healthy donor obtained from AllCells, LLC (Berkeley, CA). The sample was stored at for 48 h. Then total RNA was purified from the sample with RNeasy mini kit (Qiagen,Full Paper
2006Doshi, M;Koyanagi, M;Nakahara, M;Saeki, K;Saeki, K;Yuo, A;Identification of human neutrophils during experimentally induced inflammation in mice with transplanted CD34+ cells from human umbilical cord bloodInternational journal of hematology17050197MBR1520-3 x-ray source (Hitachi Medical, Tokyo, Japan). After 24 hours, mice received intravenous transplants of 1.8 × 105 human CB CD34+ cells (AllCells, Berkeley, CA, USA) or vehicle (saline). The purity and viability of the CB CD34+ cells were greFull Paper
2006Marion, N;Mao, J;Mesenchymal Stem Cells and Tissue EngineeringMethods in EnzymologyHuman Bone Marrow-Derived MSCs • Ficoll‐Paque—room temperature (e.g., StemCells, Inc., Vancouver, BC, Canada). • Bone marrow sample—room temperature (e.g., AllCells, LLC, Berkeley, CA). • 10 ml marrow + 5 ml DPBS + 125 U/ml heparin (total volume, 1Full Paper
2006Hawley, R;Ramezani, A;Hawley, T;Hematopoietic Stem CellsAdult Stem Cellsperipheral blood cells after informed consent in conformity with a human subjects protocol approved by an institutional review board, or purchase from a commercial source [eg, AllCells SaveFull Paper
2006Nudera, J;Mechanical stresses elicit osteogenic changes in HMSCS encapsulated in a polyethylene glycol scaffoldThesisThe samples of fresh bone marrow (Allcells, Berkeley, CA) were delivered in 10ml amoimts. Both of the patients tested negative for human immunodeficiency virus (HIV), hepatitis B SaveFull Paper
2006Crot, CA;Probing the solid-liquid interface of synthetic biomaterials via x-ray scattering techniquesThesisThe general biocompatibility of the allylamine films is compared with that of the native oxide surface of Ti by culturing human mesenchymal stems cells (hMSCs) isolated from healthy donors (AllCells, Berkeley, CA) (82). HMSCs are plated in monolayerFull Paper
2006Lee, CH;Moioli, EK;Mao, JJ;Fibroblastic differentiation of human mesenchymal stem cells using connective tissue growth factorConference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference17946857Human bone marrow samples taken from healthy donors ranging from 18 to 45 years of age were purchased from AllCells (Berkeley, CA). The bone marrows were prepared to isolate hMSCs per our previous methods [10], [11]. Briefly, the whole bone marrow saFull Paper
2006Moinnes, JJ;Vidula, N;Halim, N;Othman, SF;Ultrasound accelerated bone tissue engineering monitored with magnetic resonance microscopyConference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference17946836Mesenchymal stem cells (MSCs) isolated from fresh adult human bone marrow were provided by AllCells (AllCells LLC, Berkeley, CA). Nucleated cells were expanded via incubation SaveFull Paper
2006Dravid, G;Hammond, H;Cheng, L;Culture of human embryonic stem cells on human and mouse feeder cellsMethods in molecular biology (Clifton, N.J.)16881511mitomycin C-treated (Speciality Media; cat. no. PMEF-CF [mitomycin-treated]) (see Note 5 ). 3. Normal breast feeder cells: CCD-1087sk (American Type Culture Collection, Manassas, VA; cat. no. CRL-2104). 4. Bone marrow mononuclear cells (AllCells; LFull Paper
2006MacDonald, M;The DataGridViewPro .NET 2.0 Windows Forms and Custom Controls in C#Similar to AllCells, except the DataGridView only considers the rows that are currently visible at the time the property is set. This option is used to improve performance for large grids. SaveFull Paper
2006Crossman, LC;Predictors of imatinib response in patients with chronic myeloid leukaemiaThesisallowing us to compare the groups pre-imatinib. All patients consented to enter this study (Appendix 2). Four samples of healthy BM MNC's were obtained from AllCells (Berkeley, CA), to act as normal controlsFull Paper
2006Chen, C;Megakaryocytic* transcriptional program and developmental plasticityThesisMk and G cell cultures were initiated with fresh G-CSF-mobilized human peripheral blood CD34+ cells from normal donors (AllCells; Berkeley, CA). For Mk cultures, cells were cultured in X-VIVO 20 serum-free medium (BioWhittaker; Walkersville, MD) supFull Paper
2006Yourek, G;Effects of differentiation and shears stress on stem cell gene expression and nanomechanicsThesisWhole bone marrow (AllCells, Berkeley, CA) was mixed with "RosetteSep®" (StemCell Technologies; Vancouver, BC) (50 pL per 1 mL of bone marrow) and incubated for 20 minutes at room temperature (RT). The "RosetteSep®" antibody cocktail crosslinks unwanFull Paper
2006Clark, PA;Modulating bone ingrowth into titanium implants by growth factor delivery and mechanical loadingThesisHuman mesenchymal stem cell isolation and expansion Human mesenchymal stem cells (hMSCs) were isolated from bone marrow. Whole human bone marrow was purchased commercially (AllCells LLC, Berkeley, CA). Negative selection (RosetteSep, StemCellFull Paper
2006Charoonpatrapong, K;The roles of nuclear matrix protein 4 and high mobility group box 1 in mediating the osteoblast response to anabolic agentsThesisHuman cord blood-derived osteoclastogenesis was performed as described previously (Fuson & Galvin, 2002) with slight modification. Frozen human cord blood mononuclear cells (CBMNCs) were purchased from AllCells (Berkeley, CA). CBMNCs were quickly thaFull Paper
2005De Vry, CG;Valdez, M;Lazarov, M;Muhr, E;Buelow, R;Fong, T;Iyer, S;Topical application of a novel immunomodulatory peptide, RDP58, reduces skin inflammation in the phorbol ester-induced dermatitis modelJ. Invest. Dermatol.1611778810% neutral-buffered formalin (Fisher Scientific). The preparation of tissues by embedding in paraffin, sectioning, and staining was carried out by QualTek Molecular Laboratories (Santa Barbara, California). A series of ear crossFull Paper
2005Finnberg, N;Gruber, JJ;Fei, P;Rudolph, D;Bric, A;Kim, SH;Burns, TF;Ajuha, H;Page, R;Wu, GS;Chen, Y;McKenna, WG;Bernhard, E;Lowe, S;Mak, T;El-Deiry, WS;DR5 knockout mice are compromised in radiation-induced apoptosisMol. Cell. Biol.15713653... ts separated on a 2% agarose gel. Mean band density was determined with NIH Image 1.62 and normalized to the band density of 18S rRNA. IN SITU HYBRIDIZATION. In situ hybridization was performed by Phylogeny Inc. as described (17). Briefly, fetFull Paper
2005Schirmer, A;Gadkari, R;Reeves, CD;Ibrahim, F;DeLong, EF;Hutchinson, CR;Metagenomic analysis reveals diverse polyketide synthase gene clusters in microorganisms associated with the marine sponge Discodermia dissolutaAppl. Environ. Microbiol.16085882We thank Dan Morse (University of California, Santa Barbara) and David Myles for obtaining Discodermia dissoluta, Steve Bernstein (QualTek Molecular Laboratories, Santa Barbara, CA) for help with microscopy on sponge thin sections, and Ralph Reid forFull Paper
2005Pyle-Chenault, RA;Stolk, JA;Molesh, DA;Boyle-Harlan, D;McNeill, PD;Repasky, EA;Jiang, Z;Fanger, GR;Xu, J;VSGP/F-spondin: a new ovarian cancer markerTumour Biol.16103746Rockford, Ill., USA). Immunohistochemistry was performed with affinity-purified rabbit polyclonal antibody on formalin-fixed, paraffin-embedded tissues by QualTek Molecular Laboratories (Santa Barbara, Calif., USA). Steam heatFull Paper
2005Wang, B;Berger, M;Masters, G;Albone, E;Yang, Q;Sheedy, J;Kirksey, Y;Grimm, L;Wang, B;Singleton, J;Soltis, D;Radiotherapy of human xenograft NSCLC tumors in nude mice with a 90Y-labeled anti-tissue factor antibodyCancer Biother. Radiopharm.15989475An IHC study of TF expression in human tissues was performed at QualTek Labs (Newton, PA). Briefly, formalin-fixed, paraffin-embedded sec- tions from human NSCLC tumors and different normal human tissues were taken from QualTek's human tissue bankFull Paper
2005Urnov, FD;Miller, JC;Lee, YL;Beausejour, CM;Rock, JM;Augustus, S;Jamieson, AC;Porteus, MH;Gregory, PD;Holmes, MC;Highly efficient endogenous human gene correction using designed zinc-finger nucleasesNature15806097Human CD4+ T cells (AllCells) were grown according to the supplier's instructions. Cells were activated using 20 Units per ml IL-2 (R&D Systems) and αCD3/CD28 beads (Miltenyi Biotec, T Cell Activation/Expansion kit), and transfected with ZFN and donoFull Paper
2005Freud, AG;Becknell, B;Roychowdhury, S;Mao, HC;Ferketich, AK;Nuovo, GJ;Hughes, TL;Marburger, TB;Sung, J;Baiocchi, RA;Guimond, M;Caligiuri, MA;A human CD34(+) subset resides in lymph nodes and differentiates into CD56bright natural killer cellsImmunity15780987Fresh human BM was donated or purchased from AllCells, LLC (Berkeley, CA) and was received within 24 hr of harvest. PB leukopaks were obtained from the American Red Cross. BM SaveFull Paper
2005Neviani, P;Santhanam, R;Trotta, R;Notari, M;Blaser, BW;Liu, S;Mao, H;Chang, JS;Galietta, A;Uttam, A;Roy, DC;Valtieri, M;Bruner-Klisovic, R;Caligiuri, MA;Bloomfield, CD;Marcucci, G;Perrotti, D;The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABL-regulated SET proteinCancer cell16286244The oncogenic BCR/ABL kinase activity induces and maintains chronic myelogenous leukemia (CML). We show here that, in BCR/ABL-transformed cells and CML blast crisis (CML-BC) SaveFull Paper
2005Crossman, LC;Druker, BJ;Deininger, MW;Pirmohamed, M;Wang, L;Clark, RE;hOCT 1 and resistance to imatinibBlood16033955mononuclear cells (MNCs) cryopreserved immediately prior to imatinib therapy, and all NRs had BM MNCs stored after 9 to 15 months of imatinib treatment. Four normal BM MNC samples were obtained from AllCells (Berkeley, CA)Full Paper
2005Corbin, AS;Demehri, S;Griswold, IJ;Wang, Y;Metcalf, CA;Sundaramoorthi, R;Shakespeare, WC;Snodgrass, J;Wardwell, S;Dalgarno, D;Iuliucci, J;Sawyer, TK;Heinrich, MC;Druker, BJ;Deininger, MW;In vitro and in vivo activity of ATP-based kinase inhibitors AP23464 and AP23848 against activation-loop mutants of KitBlood15746079Mononuclear cells from 4 independent commercially available normal bone marrow samples (AllCells, Berkeley, CA) were cultured in duplicate plates with doses of AP23464 ranging Save;Hematopoietic colony forming assays: Mononuclear cells from four inFull Paper
2005Orita, T;Tsunoda, H;Yabuta, N;Nakano, K;Yoshino, T;Hirata, Y;Ohtomo, T;Nezu, J;Sakumoto, H;Ono, K;Saito, M;Kumagai, E;Nanami, M;Kaneko, A;Yoshikubo, T;Tsuchiya, M;A novel therapeutic approach for thrombocytopenia by minibody agonist of the thrombopoietin receptorBlood15374889CD34-positive cells (AllCells, LLC, Berkeley, CA) derived from human bone marrow were cultured with rhTPO or VB22B sc(Fv)2 in the presence of 0.2 ng/mL human IL-3 (PeproTech, London, UK) for 11 days at 37°C in Iscove's Modified Dulbecco's Medium (MDMFull Paper
2005Choi, J;Simons, B;Riley, C;Klinkhammer, T;Cooke, L;Spier, C;Sud, R;Hehn, S;Garewal, H;Mahadevan, D;Transcriptosome Profiling of B-CLL Identifies WNT-3A and ROR-1 as an Autocrine Mechanism in Cell Survival.BloodFull Paper
2005Clark, RA;Yamanaka, K;Bai, M;Dowgiert, R;Kupper, TS;Human skin cells support thymus-independent T cell developmentThe Journal of clinical investigation16224538For IFN-γ treated matrices, 50 ng/ml IFN-γ (R&D Systems) was included in the culture medium from plating until day 6. On day 7, 10,000 normal human bone marrow AC133+ precursor cells (AllCells) were added to each matrix, and the unit was cultured inFull Paper
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2005O'Hare, T;Walters, DK;Stoffregen, EP;Sherbenou, DW;Heinrich, MC;Deininger, MW;Druker, BJ;Combined Abl inhibitor therapy for minimizing drug resistance in chronic myeloid leukemia: Src/Abl inhibitors are compatible with imatinibClinical cancer research : an official journal of the American Association for Cancer Research16203792Samples were obtained with informed consent from four CML patients or from a tissue repository (AllCells, Berkeley, CA). The CML samples were screened for kinase domain mutations SaveFull Paper
2005Kikuchi, J;Ozaki, H;Nonomura, C;Shinohara, H;Iguchi, S;Nojiri, H;Hamada, H;Kiuchi, A;Nakamura, M;Transfection of antisense core 2 beta1,6-N-acetylglucosaminyltransferase-1 cDNA suppresses selectin ligand expression and tissue infiltration of B-cell precursor leukemia cellsLeukemia16179912(CB) was drawn from umbilical cords of cesarean section and normal full-term deliveries. Mononuclear cells (MNCs) were enriched by Ficoll density gradients and subjected to analysis. Bone marrow (BM) derived MNCs were purchased from AllCells (BerkeleFull Paper
2005D'Costa, J;Chaudhuri, S;Civin, CI;Friedman, AD;CBFbeta-SMMHC slows proliferation of primary murine and human myeloid progenitorsLeukemia15815715Human cord blood CD34+ cells (AllCells Inc., San Mateo, CA, USA) or cryopreserved human mobilized peripheral blood CD34+ cells from the Johns Hopkins cell bank (from donors who gave informed consent on an IRB-approved protocol) were placed directly iFull Paper
2005Leugers, S;Allison, D;Pronold, B;Rennebeck, G;Van Zant, G;Tario, J;Swartzwelder, F;Donahue, L;Stemline Hematopoietic Stem Cell Expansion Medium, a Serum-Free Medium for the Expansion of CD34+ Hematopoietic Stem Cells and ProgenitorsAnimal Cell Technology Meets GenomicsCell Preparation. For all experiments, cryopreserved, human CD34+ cells were obtained from independent suppliers (Stemgenix; AllCells, LLC) and were handled in a manner consistent with the supplier’s instructions with regard to storage and reconstituFull Paper
2005Wang, R;Arevalo-Herrera, M;Gardner, MJ;Bonelo, A;Carlton, JM;Gomez, A;Vera, O;Soto, L;Vergara, J;Bidwell, SL;Domingo, A;Fraser, CM;Herrera, S;Immune responses to Plasmodium vivax pre-erythrocytic stage antigens in naturally exposed Duffy-negative humans: a potential model for identification of liver-stage antigensEuropean journal of immunology15864779vivax blood-stage parasites, 2 were living in California and their PBMC were purchased from AllCells (AllCells, LLC, Berkeley, CA) and used in this study as controls. All volunteers SaveFull Paper
2005Mahadevan, D;Spier, C;Della Croce, K;Miller, S;George, B;Riley, C;Warner, S;Grogan, TM;Miller, TP;Transcript profiling in peripheral T-cell lymphoma, not otherwise specified, and diffuse large B-cell lymphoma identifies distinct tumor profile signaturesMolecular cancer therapeutics16373702Control RNA was obtained from normal peripheral B cells (AllCells, Berkeley, CA), normal peripheral T cells (AllCells), and a snap-frozen normal lymph node (Department of Pathology, SaveFull Paper
2005Siatskas, C;Underwood, J;Ramezani, A;Hawley, RG;Medin, JA;Specific pharmacological dimerization of KDR in lentivirally transduced human hematopoietic cells activates anti-apoptotic and proliferative mechanismsFASEB journal : official publication of the Federation of American Societies for Experimental Biology16076962CD34 + -selected cord blood cells were purchased from AllCells (Berkeley, CA). Cells were maintained at 37C in a humidified atmosphere of 5% CO 2 . Addition of rh stem cell factor ( SaveFull Paper
2005Rao, PE;Petrone, AL;Ponath, PD;Differentiation and expansion of T cells with regulatory function from human peripheral lymphocytes by stimulation in the presence of TGF-{beta}Journal of immunology (Baltimore, Md. : 1950)15661903For T cell differentiation using human cord blood naive CD4 + T cells, frozen CD4 + CD45RA + cells isolated using magnetic beads were obtained from Allcells. Cells were thawed and SaveFull Paper
2005Casida, JE;Quistad, GB;Serine hydrolase targets of organophosphorus toxicantsChemico-biological interactions16243304Whole human blood from seven subjects and lymphocytes (isolated by aphaeresis using a leukopac filter) from three subjects were obtained from AllCells LLC (Emeryville, CA). SaveFull Paper
2005Patel, S;Mehta-Damani, A;Shu, H;Le Pecq, JB;An analysis of variability in the manufacturing of dexosomes: implications for development of an autologous therapyBiotechnology and bioengineering16037986Peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers consisting of 111 leukopacks were obtained from AllCells Inc. (Berkeley, CA), under a protocol approved SaveFull Paper
2005Hong, L;Peptan, I;Clark, P;Mao, JJ;Ex vivo adipose tissue engineering by human marrow stromal cell seeded gelatin spongeAnnals of biomedical engineering15909657Human bone MSCs were isolated from fresh bone marrow of adult males and provided commercially by AllCells (AllCells Limited Liability Company, CA.). Nucleated cells of the fresh bone marrow were counted under inverted microscope, plated at a densityFull Paper
2005Murmann, AE;Gao, J;Encinosa, M;Gautier, M;Peter, ME;Eils, R;Lichter, P;Rowley, JD;Local gene density predicts the spatial position of genetic loci in the interphase nucleusExperimental cell research16202404Primary human bone marrow cells (CD34+ and CD33+) were purchased from AllCells Inc. (Berkeley, CA). Chinese muntjac fibroblasts, isolated from tissue of a stillbirth Chinese muntjac fawn provided by the University of Bielefeld, Department of Animal BFull Paper
2005Alhadlaq, A;Tang, M;Mao, JJ;Engineered adipose tissue from human mesenchymal stem cells maintains predefined shape and dimension: implications in soft tissue augmentation and reconstructionTissue engineering15869434Human MSCs (hMSCs) were isolated from whole bone marrow aspirates of the iliac crest of a healthy 22- year-old male donor (AllCells, Berkeley, CA). Briefly, cells were enriched by adding RosetteSep mesenchymal enrichment cocktail (StemCell TechnologiFull Paper
2005Marion, N;Liang, W;Liang, W;Reilly, G;Day, D;Rahaman, M;Mao, J;Borate Glass Supports the In Vitro Osteogenic Differentiation of Human Mesenchymal Stem CellsMechanics of Advanced Materials and StructuresBone marrow samples taken from healthy donors ranging from 18 to 45 years of age were purchased from AllCells (Berkeley, CA). Human bone marrow derived mesenchymal stem SaveFull Paper
2005Lesniak, MS;Kelleher, E;Pardoll, D;Cui, Y;Targeted gene therapy to antigen-presenting cells in the central nervous system using hematopoietic stem cellsNeurological research16354542Human G-CSF-mobilized peripheral blood or cord blood CD34z cells were obtained from AllCells (San Mateo, CA). CD34z cells were purified by immunomagnetic selection (Miltenyi SaveFull Paper
2005Ivanov, M;Kraiko, A;Numerical solution of the direct-problem of mixed nozzle flowFluid DynamicsFinally, P_I/i, k-1/2 and P-1/2, k-1/2 are determined from the resulting u and v and the given (constant for allcells) entropy and stagnationenthalpy. The calculations showed that the SaveFull Paper
2005Leung, G;Tang, H;McGuinness, R;Verdonk, E;Michelotti, J;Liu, V;Cellular Dielectric Spectroscopy: A Label-Free Technology for Drug DiscoveryJALA: Journal of the Association for Laboratory AutomationHeLa (CCL-2), and U937 (CRL-1593.2)53 were obtained from ATCC (Manassas, VA). Primary neutrophils were purchased from AllCells, LLC (Berkeley, CA). HeLa cells were grown in minimal essential medium (MEM); U937 cells were grown in RPMI-1640; CHOm1 celFull Paper
2005Owens, BM;Hawley, RG;Spain, LM;Retroviral transduction in fetal thymic organ cultureMethods in molecular medicine15492404samples are also available from several commercial sources, for example, AllCells, (Berkeley, CA) or Cambrex BioScience Walkersville, (Walkersville, MD). The thymocyte progenitor population can be transduced with retroviral constructs using VSV-G-pseFull Paper
2005Varma, C;Quantitative isoforrm profiling & isoform convergenceThesisSamples of human peripheral blood purified B cells, human cord blood purified B cells, and human adult whole bone marrow cells derived from normal (i.e. nondiseased) individuals were purchased from ALLCELLS, LLC, Berkeley, California USA. Each sampleFull Paper
2005Gao, J;Quantitative Analysis of Chromosome/Gene Spatial DistributionExperimental Cell ResearchCD34+ and CD33+ cells were primary hematopoietic stem cells and myeloid cells, respectively, (purchased from AllCells, LLC., CA) isolated from bone marrow with a MACS kit, purity of SaveFull Paper
2005Gilison, D;The role of telomerase expression in normal development and cancerThesisFresh human bone marrow was obtained with informed consent from healthy volunteers (AllCells, and Department of Bone Marrow Transplantation, Stanford University School of Medicine). Fresh human cord blood was obtained with informed consent (DepartmenFull Paper
2005Windgassen, D;Der technischen Fakultaet der Universitaet Erlangen-Nuernberg zur Erlangung des GradesThesisHealthy-donor PBMCs (AllCells, Berkeley, CA) were used to select T-cell subsets (CD4(+) and CD8(+) negative selections, Miltenyi Biotech, BergischGladbach, Germany). Briefly, non-CD4(+), CD8(+), i.e. CD4(+) or CD8(+) T cells, γ/δ T cells, B cells, NKFull Paper
2004Douglas, SA;Naselsky, D;Ao, Z;Disa, J;Herold, CL;Lynch, F;Aiyar, NV;Identification and pharmacological characterization of native, functional human urotensin-II receptors in rhabdomyosarcoma cell linesBr. J. Pharmacol.15210573Search for more papers by this author. Frank Lynch QualTek Molecular Laboratories Inc., Newtown, PA 18940, USA Search for more papers by this author. Frank Lynch QualTek Molecular Laboratories Inc., Newtown, PA 18940, USA ;nch Frank 2 Aiyar NaFull Paper
2004Bikle, DD;Chang, S;Crumrine, D;Elalieh, H;Man, MQ;Choi, EH;Dardenne, O;Xie, Z;Arnaud, RS;Feingold, K;Elias, PM;25 Hydroxyvitamin D 1 alpha-hydroxylase is required for optimal epidermal differentiation and permeability barrier homeostasisJ. Invest. Dermatol.15102089Peroxidase activity was revealed with DAB substrate (QualTek Laboratories, Santa Barbara, California) followed by counterstaining with methyl green or hematoxylin. Omitting the first antibodies resulted in no signal, indicating the specificity of immFull Paper
2004Yu, J;He, S;Friedman, JS;Akimoto, M;Ghosh, D;Mears, AJ;Hicks, D;Swaroop, A;Altered expression of genes of the Bmp/Smad and Wnt/calcium signaling pathways in the cone-only Nrl-/- mouse retina, revealed by gene profiling using custom cDNA microarraysJ. Biol. Chem.15292180The labeled transcripts were subjected to alkali hydrolysis (to give an average size of 70 nucleotides) and then hybridized overnight to 5- to 7-μm serial sections of adult mouse retina using standard protocols (Phylogeny Inc., Columbus, OH)Full Paper
2004Lynch, F;The use of an interactive computerized daily schedule in a busy interventional radiology practice increases efficiencyJ Am Coll Radiol17411739Full Paper
2004Kalos, M;Askaa, J;Hylander, BL;Repasky, EA;Cai, F;Vedvick, T;Reed, SG;Wright, GL;Fanger, GR;Prostein expression is highly restricted to normal and malignant prostate tissuesProstate15176054We thank Patrick Roche at the Mayo Clinic and Frank Lynch at Qualtek for performing immunohistochemistry experiments and Masazumi Matsamura for initial work on the generation of the 10E3-G4-D3 monoclonal antibodyFull Paper
2004Tasheva, ES;Ke, A;Conrad, GW;Analysis of the expression of chondroadherin in mouse ocular and non-ocular tissuesMol. Vis.15332017mmol, Amersham Biosciences Corp., Piscataway, NJ). For the ISH experiments, custom service from Phylogeny Inc. (Co- lumbus, OH) was used as previously described [30]. Antibodies: A polyclonal antibody against mouseFull Paper
2004Tasheva, ES;Ke, A;Deng, Y;Jun, C;Takemoto, LJ;Koester, A;Conrad, GW;Differentially expressed genes in the lens of mimecan-null miceMol. Vis.15215744secondary antibody was reacted with avidin-biotin-peroxidase complex solution (Vector Laboratories Inc., Burlingame, CA), with immunoreactivity developed in 3,3'-diaminobenzidine (DAB). In situ hybridization: For the ISH experiments, the cus- tom serFull Paper
2004Seo, BM;Miura, M;Gronthos, S;Bartold, PM;Batouli, S;Brahim, J;Young, M;Robey, PG;Wang, CY;Shi, S;Investigation of multipotent postnatal stem cells from human periodontal ligamentLancet (London, England)15246727BMSSCs were obtained from a commercially available resource (AllCells LLC, Berkeley, CA, USA). All primary cells used in this study were at 2-4 passages. For each experiment, the SaveFull Paper
2004Griswold, IJ;Shen, LJ;La Rosée, P;Demehri, S;Heinrich, MC;Braziel, RM;McGreevey, L;Haley, AD;Giese, N;Druker, BJ;Deininger, MW;Effects of MLN518, a dual FLT3 and KIT inhibitor, on normal and malignant hematopoiesisBlood15242881Bone marrow mononuclear cells (5 × 104) obtained either commercially (n = 3) (AllCells, San Diego, CA) or from a volunteer donor (n = 1) were plated in methylcellulose media containing 50 ng/mL recombinant human (rh) stem cell factor, 10 ng/mL granulFull Paper
2004Geddis, AE;Kaushansky, K;Megakaryocytes express functional Aurora-B kinase in endomitosisBlood15130946stimulated donors were purchased (Cambrex, East Rutherford, NJ, or AllCells, Berkley, CA). Cells were thawed according to the manufacturer's directions and cultured for 10 to 14 days in StemPro (Invitrogen) containing recombinant human TPO (rhTPO; 50Full Paper
2004Davis, BM;Humeau, L;Slepushkin, V;Binder, G;Korshalla, L;Ni, Y;Ogunjimi, EO;Chang, LF;Lu, X;Dropulic, B;ABC transporter inhibitors that are substrates enhance lentiviral vector transduction into primitive hematopoietic progenitor cellsBlood15059841(> 98% purity) derived from adult bone marrow, adult granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood, and cord blood were purchased from AllCells (Berkeley, SaveFull Paper
2004Lu, SJ;Li, F;Vida, L;Honig, GR;CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic gene expression patternBlood14962900cells were obtained. Normal human BM was purchased from AllCells (Berkeley, CA); CD34+CD38- cells were purified as described previously in this paragraph. Figure 1 shows the fluorescence-activated cell sorter (FACS) profiles that define the gates forFull Paper
2004Morrissey, P;George, T;Basiji, D;Frost, K;Ortyn, W;Williams, D;Cell Classification in Human Peripheral Blood Using the Amnis ImageStream® System.BloodHuman peripheral blood was obtained from AllCells (Berkeley, CA). Whole blood was stained for 15 minutes on ice with FITC conjugated anti-CD45 mAb and PE conjugated anti-CD14 SaveFull Paper
2004Mahadevan, D;Spier, C;Croce, K;Miller, S;George, B;Riley, C;Warner, S;Grogan, T;Miller, T;Gene Expression Profiling of Peripheral T-Cell Lymphoma (PTCL, NOS) and Comparison to Diffuse Large B-Cell Lymphoma (DLBCL).BloodFull Paper
2004Masubuchi, N;May, RD;Atsumi, R;A predictive model of human myelotoxicity using five camptothecin derivatives and the in vitro colony-forming unit granulocyte/macrophage assayClinical cancer research : an official journal of the American Association for Cancer Research15475463Cells were then washed in IMDM/5 and adjusted to the appropriate cell concentration. Human marrow samples were obtained from AllCells, LLC (Berkeley, CA) from donors that had given informed consent. The age of the 3 donors ranged from 20 to 45 years.Full Paper
2004Ibañez, V;Sharma, A;Buonamici, S;Verma, A;Kalakonda, S;Wang, J;Kadkol, S;Saunthararajah, Y;AML1-ETO decreases ETO-2 (MTG16) interactions with nuclear receptor corepressor, an effect that impairs granulocyte differentiationCancer research15231665Retrovirus-containing supernatant was added to human cord blood CD34-positive cells (AllCells) in retronectin (TaKaRa)-coated plates. The cord blood cells were cultured in Iscove's SaveFull Paper
2004Georgantas, RW;Tanadve, V;Malehorn, M;Heimfeld, S;Chen, C;Carr, L;Martinez-Murillo, F;Riggins, G;Kowalski, J;Civin, CI;Microarray and serial analysis of gene expression analyses identify known and novel transcripts overexpressed in hematopoietic stem cellsCancer research15231652The human CD34 + /CD38 − /Lin − cell subset, comprising ∼1-10% of the CD34 + cell population, contains few of the less primitive hematopoietic (lineage-committed) progenitor cells ( SaveFull Paper
2004Humeau, LM;Binder, GK;Lu, X;Slepushkin, V;Merling, R;Echeagaray, P;Pereira, M;Slepushkina, T;Barnett, S;Dropulic, LK;Carroll, R;Levine, BL;June, CH;Dropulic, B;Efficient lentiviral vector-mediated control of HIV-1 replication in CD4 lymphocytes from diverse HIV+ infected patients grouped according to CD4 count and viral loadMolecular therapy : the journal of the American Society of Gene Therapy15194057Peripheral blood from healthy donors was obtained from AllCells (Berkeley, CA, USA), and uninfected or HIV+ study subject PBMCs were isolated by Ficoll-Hypaque density gradient separation. Study subjects numbered J23 and higher and U01 had their blooFull Paper
2004Davis, BM;Humeau, L;Dropulic, B;In vivo selection for human and murine hematopoietic cells transduced with a therapeutic MGMT lentiviral vector that inhibits HIV replicationMolecular therapy : the journal of the American Society of Gene Therapy14759800progenitors were obtained from AllCells (Berkley, CA, USA) in accordance with an IRB protocol obtained by the company and were transduced and cultured in IMDM plus 1× BIT (Stem Cell Technologies, Vancouver, BC, Canada) in 25 ng/ml SCF, Flt-3L, and TPFull Paper
2004LeCouter, J;Zlot, C;Tejada, M;Peale, F;Ferrara, N;Bv8 and endocrine gland-derived vascular endothelial growth factor stimulate hematopoiesis and hematopoietic cell mobilizationProceedings of the National Academy of Sciences of the United States of America15548611Bv8 and endocrine-gland-derived VEGF (EG-VEGF), or prokineticins, are two highly related, secreted proteins that we previously described as selective angiogenic mitogens. Here we SaveFull Paper
2004Su, AI;Wiltshire, T;Batalov, S;Lapp, H;Ching, KA;Block, D;Zhang, J;Soden, R;Hayakawa, M;Kreiman, G;Cooke, MP;Walker, JR;Hogenesch, JB;A gene atlas of the mouse and human protein-encoding transcriptomesProceedings of the National Academy of Sciences of the United States of America15075390Tissue Preparation. Human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA), Clontech, AllCells (Berkeley, CA), Clonetics/BioWhittaker (Walk-ersville, MD), AMS Biotechnology (Abingdon, Oxfordshire, U.K.), and tFull Paper
2004Begovich, AB;Carlton, VE;Honigberg, LA;Schrodi, SJ;Chokkalingam, AP;Alexander, HC;Ardlie, KG;Huang, Q;Smith, AM;Spoerke, JM;Conn, MT;Chang, M;Chang, SY;Saiki, RK;Catanese, JJ;Leong, DU;Garcia, VE;McAllister, LB;Jeffery, DA;Lee, AT;Batliwalla, F;Remmers, E;Criswell, LA;Seldin, MF;Kastner, DL;Amos, CI;Sninsky, JJ;Gregersen, PK;A missense single-nucleotide polymorphism in a gene encoding a protein tyrosine phosphatase (PTPN22) is associated with rheumatoid arthritisAmerican journal of human genetics15208781(GenBank accession number NM_015967) was determined by kinetic RT-PCR analysis by use of total RNA from sorted hematopoeitic cells (AllCells) and selected tissues (Clontech), as described elsewhere (Rogge et al. 2000). Data for additional tissues areFull Paper
2004Lu, X;Yu, Q;Binder, GK;Chen, Z;Slepushkina, T;Rossi, J;Dropulic, B;Antisense-mediated inhibition of human immunodeficiency virus (HIV) replication by use of an HIV type 1-based vector results in severely attenuated mutants incapable of developing resistanceJournal of virology15194784Primary cell isolation, culture, transduction, and challenge. Peripheral blood from healthy donors was obtained from AllCells (Berkeley, Calif.), and lymphocytes were isolated by Ficoll-Hypaque density gradient separation. Cells were resuspended in bFull Paper
2004Chute, JP;Muramoto, G;Fung, J;Oxford, C;Quantitative analysis demonstrates expansion of SCID-repopulating cells and increased engraftment capacity in human cord blood following ex vivo culture with human brain endothelial cellsStem cells (Dayton, Ohio)14990859Purified human CB CD34+ cells were obtained from AllCells, LLC. (Berkeley, CA; http://www.allcells.com). HUBECs were subcultured at 1 × 105 cells/well in gelatin‐coated 6‐well plates (Costar; Cambridge, MA; http://www.corning.com) as previously descrFull Paper
2004Courtemanche, C;Huang, AC;Elson-Schwab, I;Kerry, N;Ng, BY;Ames, BN;Folate deficiency and ionizing radiation cause DNA breaks in primary human lymphocytes: a comparisonFASEB journal : official publication of the Federation of American Societies for Experimental Biology14597554Normal human T lymphocytes were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% dialyzed SaveFull Paper
2004Friedmann, E;Lemberg, MK;Weihofen, A;Dev, KK;Dengler, U;Rovelli, G;Martoglio, B;Consensus analysis of signal peptide peptidase and homologous human aspartic proteases reveals opposite topology of catalytic domains compared with presenilinsThe Journal of biological chemistry15385547Microarray Analysis—Human tissues, total RNA samples, and mRNA samples were obtained from Clinomics Biosciences, Inc., Clontech, AllCells, LLC, Clonetics/BioWhittaker, AMS Biotechnology, and the University of California, San Diego. The quality of allFull Paper
2004Courtemanche, C;Elson-Schwab, I;Mashiyama, ST;Kerry, N;Ames, BN;Folate deficiency inhibits the proliferation of primary human CD8+ T lymphocytes in vitroJournal of immunology (Baltimore, Md. : 1950)15322179Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen, Carlsbad, CA), supplemented with 10% dialyzed SaveFull Paper
2004Chu, PC;Wu, J;Liao, XC;Pardo, J;Zhao, H;Li, C;Mendenhall, MK;Pali, E;Shen, M;Yu, S;Taylor, VC;Aversa, G;Molineaux, S;Payan, DG;Masuda, ES;A novel role for p21-activated protein kinase 2 in T cell activationJournal of immunology (Baltimore, Md. : 1950)15187108Freshly purified PBMC (AllCells, Berkeley, CA) were incubated at 37°C for 2 h to allow the monocytes to adhere to the tissue culture flask. The cells remaining in suspension were recovered and seeded at 106 cells/ml/ well in Yssel’s medium (Gemini BiFull Paper
2004Chinnasamy, D;Fairbairn, LJ;Neuenfeldt, J;Treisman, JS;Hanson, JP;Margison, GP;Chinnasamy, N;Lentivirus-mediated expression of mutant MGMTP140K protects human CD34+ cells against the combined toxicity of O6-benzylguanine and 1,3-bis(2-chloroethyl)-nitrosourea or temozolomideHuman gene therapy15319033Lentiviral vectors are capable of efficiently transducing nondividing and slowly dividing cells, including hematopoietic stem cells, resulting in stable integration and sustained transgene SaveFull Paper
2004Lu, X;Humeau, L;Slepushkin, V;Binder, G;Yu, Q;Slepushkina, T;Chen, Z;Merling, R;Davis, B;Chang, YN;Dropulic, B;Safe two-plasmid production for the first clinical lentivirus vector that achieves >99% transduction in primary cells using a one-step protocolThe journal of gene medicine15352069Normal human blood was obtained from AllCells (Berkely, CA, USA) under the auspices of an IRB-approved protocol supplied by AllCells. Peripheral blood mononuclear cells (PBMCs) SaveFull Paper
2004Kubota, S;Kawata, K;Yanagita, T;Doi, H;Kitoh, T;Takigawa, M;Abundant retention and release of connective tissue growth factor (CTGF/CCN2) by plateletsJournal of biochemistry15598883Lower panel: Comparison of the CTGF/CCN2 contents of platelets and other CTGF/CCN2-associated tissues. Total protein extracts of normal human skin and artery were obtained commercially (Cosmo Bio Co., Ltd., Tokyo, Japan), as were human platelets fromFull Paper
2004Mashiyama, ST;Courtemanche, C;Elson-Schwab, I;Crott, J;Lee, BL;Ong, CN;Fenech, M;Ames, BN;Uracil in DNA, determined by an improved assay, is increased when deoxynucleosides are added to folate-deficient cultured human lymphocytesAnalytical biochemistry15183762Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and 1% antibiotics (penicillin/streptavidin). To ensureFull Paper
2004Murray, B;Bennett, B;Sasaki, D;Analysis of pharmacologic inhibitors of Jun N-terminal kinasesMethods in Enzymologydom donor buffy coats from the San Diego Blood Bank (San Diego, CA). Neonatal cord blood is obtained from Advanced Bioscience Resources (Alameda, CA) or as selected CD4+ cells (AllCells, Foster City, CA)Full Paper
2004Lu, SJ;Li, F;Vida, L;Honig, GR;CD34CD38 Hematopoietic Precursors Derived from Human Embryonicresearchgate.netof the total population, and a total of 2,840 CD34+ CD38- cells was obtained. Normal human BM was purchased from AllCells (AllCells, LLC, Berkeley, CA); CD34+ CD38- cells were purified as described above. Figure 1 shows the FACS profiles that defineFull Paper
2004Murmann, AE;Topology of genes in mammalian cell nuclei with special emphasis on the MLL gene and its translocation partnersThesisATCC = American Type Culture Collection, Manassas, VA. DSMZ = Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany. 3. Material 38 CD33+) were isolated by and purchased from AllCells Inc. (Berkeley, CA)Full Paper
2004Mashiyama, ST;Folate and other B-vitamins: Effects of deficiency on DNA-uracil content, chromosome damage, homocysteine, and interactions with gene polymorphismsThesisCell culture and proliferation Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and 1% antibiotics (penicFull Paper
2003Winkler, DG;Sutherland, MK;Geoghegan, JC;Yu, C;Hayes, T;Skonier, JE;Shpektor, D;Jonas, M;Kovacevich, BR;Staehling-Hampton, K;Appleby, M;Brunkow, ME;Latham, JA;Osteocyte control of bone formation via sclerostin, a novel BMP antagonistEMBO J.14633986Mouse embryos (15.5 days old) were fixed in 4% phosphate-buffered formaldehyde pH 7.2 overnight, dehydrated and embedded in paraffin. Sections (5 µm thick) were prepared and in situ hybridization performed by Phylogeny, Inc. (Columbus, OH) using [35SFull Paper
2003Wang, T;Fan, L;Watanabe, Y;McNeill, PD;Moulton, GG;Bangur, C;Fanger, GR;Okada, M;Inoue, Y;Persing, DH;Reed, SG;L523S, an RNA-binding protein as a potential therapeutic target for lung cancerBr. J. Cancer12644826ur studies indicate that L523S may be a valuable addition to the repertoire of cancer-specific targets for the development of new immunotherapeutic and perhaps other therapeutic approaches. We thank Qualtek for their expert IHC analysis. We are gratFull Paper
2003DuHadaway, JB;Lynch, FJ;Brisbay, S;Bueso-Ramos, C;Troncoso, P;McDonnell, T;Prendergast, GC;Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: diverse sites of nuclear expression and losses in prostate cancerJ. Cell. Biochem.12532338Search for more papers by this author. Frank J. Lynch QualTek Molecular Laboratories, Newtown, Pennsylvania. Search for more papers by this author. Shawn Brisbay Search for more papers by this author. Frank J. Lynch QualTek Molecular Laboratories,Full Paper
2003Tinkelenberg, BA;Fazzone, W;Lynch, FJ;Ladner, RD;Identification of sequence determinants of human nuclear dUTPase isoform localizationExp. Cell Res.1279918015 min), DUT415 primary antibody incubation (25 min), biotinylated goat anti-mouse IgG secondary antibody (25 min), peroxidase-conjugated ABC-Elite (25 min, Vector Labs, Burlingame, CA, USA), and DAB (diaminobenzidine) chromogen (15 min, QualTek MoleFull Paper
2003Gibbs, WW;Untangling the roots of cancerSci. Am.12840947SCIENCE PHOTO LIBRARY; FRANK LYNCH QualTek Molecular Laboratories; ANDREJS LIEPINS/SPL; CNRI/SPL; SPL 1. GROWTH EVEN IN THE ABSENCE OF NORMAL "GO" SIGNALS Most normal cells wait for an external message before dividingFull Paper
2003Wang, A;Clapper, J;Guderian, JA;Foy, TM;Fanger, GR;Retter, MW;Skeiky, YA;A novel method for increasing the expression level of recombinant proteinsProtein Expr. Purif.12821330visualize antigen expression. Slides were counterstained with hematoxylin to visualize cell nuclei. We would like to acknowledge and thank Qualtek for immunohistochemistry results. FACS analysis. HEK293 cells were transientlyFull Paper
2003Weigel-Kelley, KA;Yoder, MC;Srivastava, A;Alpha5beta1 integrin as a cellular coreceptor for human parvovirus B19: requirement of functional activation of beta1 integrin for viral entryBlood12907437Human bone marrow-derived highly purified CD71 + primary erythroid progenitor cells were purchased from AllCells (Berkeley, CA). Recombinant parvovirus B19-Luc vector stocks SaveFull Paper
2003Francisco, JA;Cerveny, CG;Meyer, DL;Mixan, BJ;Klussman, K;Chace, DF;Rejniak, SX;Gordon, KA;DeBlanc, R;Toki, BE;Law, CL;Doronina, SO;Siegall, CB;Senter, PD;Wahl, AF;cAC10-vcMMAE, an anti-CD30-monomethyl auristatin E conjugate with potent and selective antitumor activityBlood12714494and goat-antihuman FITC were obtained from Jackson ImmunoResearch (West Grove, PA). Anti-CD30 mAb Ki-1 was from Accurate Chemicals (Westbury, NY). cBR96, a human chimeric IgG1κ (Seattle Genetics) was used as a chimeric, isotype-matched control. FreshFull Paper
2003Glockner, A;Optimal consistency protocols for replicated filesEighth Annual International Phoenix Conference on Computers and Communications. 1989 Conference ProceedingsPlacement continues until either allcells have been placed, or a number of iterations has beenperformed. If, during cell placement, a valid location for acell cannot be identified, the SaveFull Paper
2003Lee, J;Elkahloun, AG;Messina, SA;Ferrari, N;Xi, D;Smith, CL;Cooper, R;Albert, PS;Fine, HA;Cellular and genetic characterization of human adult bone marrow-derived neural stem-like cells: a potential antiglioma cellular vectorCancer research14695205RBCs from human adult (donors, 20-40 years old) bone marrow cells purchased from AllCells (Berkely, CA) were depleted by density gradient, and the remaining cells were plated in uncoated tissue culture plastic dishes. After 10 days of culture in 15%Full Paper
2003Yu, X;Zhan, X;D'Costa, J;Tanavde, VM;Ye, Z;Peng, T;Malehorn, MT;Yang, X;Civin, CI;Cheng, L;Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cellsMolecular therapy : the journal of the American Society of Gene Therapy12788657Normal human placental/umbilical CB CD34+ cells, purchased from AllCells (San Mateo, CA), had been purified by immunomagnetic selection (Miltenyi Biotech, Auburn, CA) and were90% CD34+ upon reanalysis by FACS. CB CD34+ cells were cultured at 2.5-5 ×Full Paper
2003Thach, DC;Shaffer, KM;Ma, W;Stenger, DA;Assessing the feasibility of using neural precursor cells and peripheral blood mononuclear cells for detection of bioactive Sindbis virusBiosensors & bioelectronics12782470Fresh normal PBMC were purchased from AllCells , LLC. The cells were received in Dubecco's PBS with 0.5% bovine serum albumin (BSA) and 2 mM EDTA. The cells were spun SaveFull Paper
2003Russell, RS;Roldan, A;Detorio, M;Hu, J;Wainberg, MA;Liang, C;Effects of a single amino acid substitution within the p2 region of human immunodeficiency virus type 1 on packaging of spliced viral RNAJournal of virology14645555Allcells were supplemented with 10% fetal calf serum. Cord bloodmononuclear cells (CBMCs) were grown in RPMI 1640 supplemented with10% fetal calf serum and 20 U of interleukin- SaveFull Paper
2003Cheng, L;Hammond, H;Ye, Z;Zhan, X;Dravid, G;Human adult marrow cells support prolonged expansion of human embryonic stem cells in cultureStem cells (Dayton, Ohio)12634409BM samples collected from healthy and consenting human donors were purchased from AllCells Company (Berkeley, CA; http://www.allcells.com). The use of anonymous primary human cells for laboratory research was approved by the Internal Review Board ofFull Paper
2003Hsu, DH;Paz, P;Villaflor, G;Rivas, A;Mehta-Damani, A;Angevin, E;Zitvogel, L;Le Pecq, JB;Exosomes as a tumor vaccine: enhancing potency through direct loading of antigenic peptidesJournal of immunotherapy (Hagerstown, Md. : 1997)12973033CD14+ monocyte-enriched adherent cells from healthy donor leukopacks acquired from Allcells (Berkeley, CA) were cultured in ultrafiltered AIMV medium (Invitrogen) supplemented SaveFull Paper
2003Hofstra, CL;Desai, PJ;Thurmond, RL;Fung-Leung, WP;Histamine H4 receptor mediates chemotaxis and calcium mobilization of mast cellsThe Journal of pharmacology and experimental therapeutics12626656Human HMC-1 and WEHI-3 cells were purchased from American Type Culture Collection (Manassas, VA). Human CD34 cord blood cells were from AllCells, LLC (Berkeley, CA). Basophil enrichment kit and serum-free medium were from Stem Cells Technologies (VanFull Paper
2003Greco, NJ;Lee, WR;Moroff, G;Increased transmigration of G-CSF-mobilized peripheral blood CD34+ cells after overnight storage at 37 degrees CTransfusion14617318Samples from UCSD and AllCells LLC were shipped overnight (ON) at 1 to 6C and utilized in experiments upon receipt the following morning (referred to as Day 1). PBPC samples from SaveFull Paper
2003Scherer, A;Krause, A;Walker, JR;Sutton, SE;Serón, D;Raulf, F;Cooke, MP;Optimized protocol for linear RNA amplification and application to gene expression profiling of human renal biopsiesBioTechniques12661160Human PBMCs were purchased from Allcells (Oakland, CA, USA). Cells were subjected to RNA purification with RNeasy® (Qiagen, Valencia, CA, USA). The RNA was eluted with 80 µL RNase-free water (Ambion,Full Paper
2003Onguru, O;Celasun, B;Gunhan, O;Comparison of DNA ploidy and nuclear morphometric parameters with the conventional prognostic factors in transitional cell carcinomasThe Tohoku journal of experimental medicine12703658ThemeasurementofNA10in thisstudydemonstratedthemeannucleararea ofthebiggest10cellsamong the allcells measuredatthenucleussuspensionforeach case. Thus, SaveFull Paper
2003Yamaguchi, H;Aridgides, LJ;Zeng, W;Osgood, C;Young, NS;Liu, JM;Genetic and transcriptional analysis of spindle checkpoint genes in bone marrow failure patientsBlood cells, molecules & diseases12737950peripheral blood (PB)-MNCs was extracted using an RNAzol kit (Biotex Laboratories, Houston, TX) or Trizol (Life Technologies). Total RNA from purified T cell, B cell, and neutrophil fractions was purchased (AllCells, LLC, Berkeley, CA)Full Paper
2003Christensen, JL;Mechanisms and mediators of hematopoietic stem cell fateThesisHuman bone marrow was obtained from normal donors (Allcells, Oakland, CA). Bone marrow was diluted in Hanks balanced salt solution, and 20ml was layered onto 15ml of ficoll (Ficoll-Paque PLUS (Amersham Phamacia Biotech), and centrifuged for 30 minuteFull Paper
2003Chu, P;Pardo, J;Zhao, H;Li, CC;Pali, E;Shen, MM;Qu, K;Yu, SX;Huang, BC;Yu, P;Masuda, ES;Molineaux, SM;Kolbinger, F;Aversa, G;de Vries, J;Payan, DG;Liao, XC;Systematic identification of regulatory proteins critical for T-cell activationJournal of biology129749811,599 of its open reading frame; and TRAC-1, base pairs 1-509 of its open reading frame. Culture and infection of primary T lymphocytes Commercially available primary blood mononuclear cells (PBMC; AllCells LLC, Berkeley, USA) were cultured in RPMIFull Paper
2003Knowles, S;Woods, R;McWhirter, J;McCanny, J;Bit-level systolic arrays for IIR filtering[1988] Proceedings. International Conference on Systolic Arraysthroughout this paper.,The application contains four main functions as follows:,1),startPlay,function calls,setup,to set the,dimension of all DOM elements with a class name of,allCells,;,2), SaveFull Paper
2003Chow, DC;Effects of surface-immobilized stem cell factor on the differentiation and proliferation of hematopoietic cellsThesisgnetic cell separation system (Miltenyl Biotec GmbH, Bergisch Gladbach, Germany) (AllCells, Berkely, CA). Cells were cultured in the presence of soluble SCF (100 ng/ml) or surface-immobilized SCF in serum-free medium (XVIVO20. Cambrex Bio Science, WaFull Paper
2002Elias, PM;Ahn, SK;Denda, M;Brown, BE;Crumrine, D;Kimutai, LK;Kömüves, L;Lee, SH;Feingold, KR;Modulations in epidermal calcium regulate the expression of differentiation-specific markersJ. Invest. Dermatol.12445203from Vector Laboratories (Burlingame, CA). Peroxidase activity was revealed with diaminobenzidine (QualTek Laboratories, Santa Barbara, CA), followed by counterstaining with methyl green. Omission of the first antibodiesFull Paper
2002Xie, Z;Komuves, L;Yu, QC;Elalieh, H;Ng, DC;Leary, C;Chang, S;Crumrine, D;Yoshizawa, T;Kato, S;Bikle, DD;Lack of the vitamin D receptor is associated with reduced epidermal differentiation and hair follicle growthJ. Invest. Dermatol.11851870Peroxidase activity was revealed with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followed by counterstaining with methyl green or hematoxylin. Omitting the first antibodies resulted in no signal, indicating the specificity oFull Paper
2002Fanger, GR;Houghton, RL;Retter, MW;Hendrickson, RC;Babcook, J;Dillon, DC;Durham, MD;Reynolds, LD;Johnson, JC;Carter, D;Fleming, TP;Roche, PC;Persing, DH;Reed, SG;Detection of mammaglobin in the sera of patients with breast cancerTumour Biol.12499777Acknowledgments We thank Frank Lynch at Qualtek for immunohistochemistry efforts and expertise. Work was supported in part by USPHS grant CA76227 (TPF), and NIH grants CA-75794 (RLH) and CA- 86673 (DCD) References 1 Donegan W, Spratt J: Cancer of theFull Paper
2002Kumar, S;Hanning, CR;Brigham-Burke, MR;Rieman, DJ;Lehr, R;Khandekar, S;Kirkpatrick, RB;Scott, GF;Lee, JC;Lynch, FJ;Gao, W;Gambotto, A;Lotze, MT;Interleukin-1F7B (IL-1H4/IL-1F7) is processed by caspase-1 and mature IL-1F7B binds to the IL-18 receptor but does not induce IFN-gamma productionCytokine12096920Full Paper
2002Mulero, JJ;Boyle, BJ;Bradley, S;Bright, JM;Nelken, ST;Ho, TT;Mize, NK;Childs, JD;Ballinger, DG;Ford, JE;Rupp, F;Three new human members of the lipid transfer/lipopolysaccharide binding protein family (LT/LBP)Immunogenetics12185532The BPIL2 probe was derived from a 414-nt coding sequence between the primers 5'-CATTATTGCAAGTGAAGTCAAAGC-3' and 5'-ACCATGA- AGGGCTGGGACAAGATG-3'. QualTek Molecular labs (Santa Barbara, Calif.) performed the automated in situ hybridization us- ing aFull Paper
2002Zhao, Z;Yaar, R;Ladd, D;Cataldo, LM;Ravid, K;Overexpression of A3 adenosine receptors in smooth, cardiac, and skeletal muscle is lethal to embryosMicrovasc. Res.11749073Page 4. tion, generating either a sense or an antisense strand template for in vitro transcription (with EcoRV or HindIII, respectively). In situ hybridization was performed by Dr. Adel Mikhail (Phylogeny, Inc., Columbus, OH) as described by Lyons etFull Paper
2002Hevehan, DL;Miller, WM;Papoutsakis, ET;Differential expression and phosphorylation of distinct STAT3 proteins during granulocytic differentiationBlood11861277Fresh G-CSF-mobilized peripheral blood CD34 + cells from normal donors were obtained from AllCells (Foster City, CA) within 24 hours after collection. The experimental design and SaveFull Paper
2002Cui, Y;Golob, J;Kelleher, E;Ye, Z;Pardoll, D;Cheng, L;Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cellsBlood11781219Human G-CSF-mobilized peripheral blood or cord blood CD34+ cells were obtained from AllCells (San Mateo, CA). CD34+ cells were purified by immunomagnetic selection (Miltenyi Biotec, Auburn, CA) and then cryopreserved. The purity of CD34+ cells was usFull Paper
2002Tuttle, G;Fallahi, S;Abidi, A;An 8 b CMOS vector A/D converter1993 IEEE International Solid-State Circuits Conference Digest of Technical PapersThe two pMOSFETsin allcells are diode connected, and the nMOSFET is biased by a globally-applied reference gate voltage. The input is then switched to the prompt vector X, and the SaveFull Paper
2002Kang, SJ;Cresswell, P;Regulation of intracellular trafficking of human CD1d by association with MHC class II moleculesThe EMBO journal11927549DCs were derived from monocytes as described (Sallusto and Lanzavecchia, 1994). Briefly, monocytes from peripheral blood mononuclear cells (8 × 108 cells) (AllCells, LLC, Berkeley, CA) were attached to tissue culture flasks in 20% FBS/RPMI 1640 twiceFull Paper
2002Manz, MG;Miyamoto, T;Akashi, K;Weissman, IL;Prospective isolation of human clonogenic common myeloid progenitorsProceedings of the National Academy of Sciences of the United States of America12193648Bone Marrow and Cord Blood Samples. Fresh human bone marrow (donor age, 20-47 years) was obtained with informed consent from healthy volunteers (AllCells, Berkeley, CA), allogeneic bone marrow donors (Department of Bone Marrow Transplantation, StanfoFull Paper
2002Mahmud, DL;G-Amlak, M;Deb, DK;Platanias, LC;Uddin, S;Wickrema, A;Phosphorylation of forkhead transcription factors by erythropoietin and stem cell factor prevents acetylation and their interaction with coactivator p300 in erythroid progenitor cellsOncogene11896584Primary human erythroid progenitors were derived by in vivo culture of CD34+ cells isolated from peripheral blood. Growth factor mobilized peripheral blood collected from normal donors was purchased from AllCells, LLC (Berkeley, CA, USA). These bloodFull Paper
2002Yang, H;Miller, WM;Papoutsakis, ET;Higher pH promotes megakaryocytic maturation and apoptosisStem cells (Dayton, Ohio)12110701Cultures were initiated with adult human CD34+ cells selected from apheresis products obtained from normal donors (AllCells; Berkeley, CA; http://www.allcells.com) or cancer patients (Northwestern Memorial Hospital, Chicago, IL) following stem cell mFull Paper
2002Kim, H;Whartenby, KA;Georgantas, RW;Wingard, J;Civin, CI;Human CD34+ hematopoietic stem/progenitor cells express high levels of FLIP and are resistant to Fas-mediated apoptosisStem cells (Dayton, Ohio)11897874Human placental/umbilical cord blood (CB) CD34+ cell preparations, purified by immunomagnetic selection following manufacturer's instructions (Miltenyi Biotechnologies; Auburn, CA; http://www.miltenyibiotec.com) and then cryopreserved, were purchasedFull Paper
2002Welm, AL;Timchenko, NA;Ono, Y;Sorimachi, H;Radomska, HS;Tenen, DG;Lekstrom-Himes, J;Darlington, GJ;C/EBPalpha is required for proteolytic cleavage of cyclin A by calpain 3 in myeloid precursor cellsThe Journal of biological chemistry12105198Bone marrow was isolated from both femurs of adult animals as described (25). CD33+ and CD15+ bone marrow cells were obtained from AllCells, L.L.C. skeletal muscle was harvested from adult animals as a control for calpain 3 Northern analysisFull Paper
2002Jacobs-Helber, SM;Abutin, RM;Tian, C;Bondurant, M;Wickrema, A;Sawyer, ST;Role of JunB in erythroid differentiationThe Journal of biological chemistry11726656Growth factor-mobilized peripheral blood collected from normal donors was purchased from AllCells, LLC (Berkeley, CA). CD34 + cells were isolated from growth factor-mobilized SaveFull Paper
2002Harrisson, F;Cellular origin of the basement membrane in embryonic chicken/quail chimeras.International Journal of Developmental Biologysuch as Quailnucleoli, are not clear in allcells. The drawings show the place where the photomicrographs were taken. The dashed area indicates the locatIOn of the mesoblast. SaveFull Paper
2002Sharma, A;The role of the hiwi gene in human hematopoiesisThesisLouis, MO) and stained first with biotin-conjugated K6.1 (20 ug/ml). washed, and labeled with Miltenyi streptavidin-conjugated iron microbeads (Miltenyi Biotec) and selected as described above according to manufacturer's instructions. Cord blood (CB)Full Paper
2001Afar, DE;Vivanco, I;Hubert, RS;Kuo, J;Chen, E;Saffran, DC;Raitano, AB;Jakobovits, A;Catalytic cleavage of the androgen-regulated TMPRSS2 protease results in its secretion by prostate and prostate cancer epitheliaCancer Res.11245484We thank Drs. Robert Eisenman, Owen Witte, and William Isaacs for helpful suggestions and critical reading of the manuscript; Frank Lynch and Page Erickson at QualTek Molecular Laboratories for their expertise in immunohistochemistry; and Celeste JonFull Paper
2001Xu, J;Kalos, M;Stolk, JA;Zasloff, EJ;Zhang, X;Houghton, RL;Filho, AM;Nolasco, M;Badaró, R;Reed, SG;Identification and characterization of prostein, a novel prostate-specific proteinCancer Res.11245466Immunohistochemical Studies. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissues by QualTek Molecular Laboratories using the prostein-specific mouse monoclonal antibody 10E3- G4-D3. Chromosome Localization. The GenFull Paper
2001Wang, T;Fan, L;Watanabe, Y;McNeill, P;Fanger, GR;Persing, DH;Reed, SG;L552S, an alternatively spliced isoform of XAGE-1, is over-expressed in lung adenocarcinomaOncogene11753648An affinity purified anti-L552S polyclonal antibody was subjected to immunohistochemistry analysis by QualTek Molecular Laboratories (Santa Barbara, CA, USA). In all cases, four micron sections of formalin fixed, paraffin-embedded tissues were used.Full Paper
2001Lin, H;Ho, AS;Haley-Vicente, D;Zhang, J;Bernal-Fussell, J;Pace, AM;Hansen, D;Schweighofer, K;Mize, NK;Ford, JE;Cloning and characterization of IL-1HY2, a novel interleukin-1 family memberJ. Biol. Chem.11278614The anti-CD20 monoclonal antibody was purchased from DAKO (Carpenteria, CA), and the anti-Ki67 was obtained from Coulter Immunotech (Miami, FL). Immunohistochemistry was performed at QualTek Molecular Systems, IncFull Paper
2001Kutty, RK;Kutty, G;Samuel, W;Duncan, T;Bridges, CC;El-Sherbeeny, A;Nagineni, CN;Smith, SB;Wiggert, B;Molecular characterization and developmental expression of NORPEG, a novel gene induced by retinoic acidJ. Biol. Chem.11042181Immunoreactivity was detected by peroxidase staining using reagents from Dako Corp. (Carpinteria, CA). In Situ Hybridization. In situ hybridization was performed by Phylogeny Inc. following the procedure of Lyonset al. (18) as described before (19)Full Paper
2001Suerbaum, S;Lohrengel, M;Sonnevend, A;Ruberg, F;Kist, M;Allelic diversity and recombination in Campylobacter jejuniJ. Bacteriol.11274115Different genes show network-like and tree-like phylogeny in C. jejuni. In order to further analyze the population structure of C. jejuni, we used split decomposition analysis. Split decomposition was originally described by Bandelt and Dress in 1992Full Paper
2001Taylor, RE;Shows, KH;Zhao, Y;Pittler, SJ;A PDE6A promoter fragment directs transcription predominantly in the photoreceptorBiochem. Biophys. Res. Commun.11401494Mannheim). An outside commercial company (Qualtek Inc., CA) confirmed results obtained in-house using the same exper- imental probes as above with proprietary in situ hybridization re- agents and the following conditionsFull Paper
2001Ladner, RD;The role of dUTPase and uracil-DNA repair in cancer chemotherapyCurr. Protein Pept. Sci.12374095await further investigation. ACKNOWLEDGEMENTS Special thanks is extended to Dr. Frank J. Lynch at QualTek Molecular Laboratories, Santa Barbara, CA, for expertise in immunohistochemistry. Special thanks are extendedFull Paper
2001Gao, Z;Golob, J;Tanavde, VM;Civin, CI;Hawley, RG;Cheng, L;High levels of transgene expression following transduction of long-term NOD/SCID-repopulating human cells with a modified lentiviral vectorStem cells (Dayton, Ohio)11359950Normal human cord blood (CB) CD34+ cells were purchased from either AllCells (San Mateo, CA; http://www.purecell.com) or Poietics/BioWhittaker (Gaithersburg, MD; http://www.biowhittaker.com/). CD34+ cells were purified by immunomagnetic selection (Mi
2001Bonfoco, E;Li, E;Kolbinger, F;Cooper, NR;Characterization of a novel proapoptotic caspase-2- and caspase-9-binding proteinThe Journal of biological chemistry11350957Positively selected, CD19+ MACS-purified human B cells (AllCells, San Jose, CA) were resuspended at 1 × 106 cells/ml in Yssel's medium without antibody (Gemini Bioproducts, Calabasas, CA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT).
2001Alonso, A;Saxena, M;Williams, S;Mustelin, T;Inhibitory role for dual specificity phosphatase VHR in T cell antigen receptor and CD28-induced Erk and Jnk activationThe Journal of biological chemistry11085983Human bone marrow was purchased from AllCells LLC (Foster City, CA). Bone marrow leukocytes were obtained by hypotonic lysis of the erythrocytes in water for 1 min followed by washing of the cells in RPMI medium. Peripheral blood lymphocytes (∼80% T
2001西田耕介, ;高城敏美, ;木下進一, ;辻正, ;燃料電池・ガスタービン複合発電システムの性能解析 (熱工学, 内燃機関, 動力など)日本機械学会論文集 B 編段目の電 のみに蒸気を投入する方式(1段目蒸気投入 方式,1stcel1steamadditionform)は,全 段の電 に蒸 気を投入する方式(全段蒸気投入方式,Allcells Steam additionform)よりも単位燃料 Save
2001Mostafa, SS;Papoutsakis, ET;Miller, WM;Oxygen tension modulates the expression of cytokine receptors, transcription factors, and lineage-specific markers in cultured human megakaryocytesExperimental hematology11438210Cultures were initiated with adult human CD34+ cells selected from apheresis products obtained from normal donors mobilized with G-CSF (Poietics Technologies, Gaithersburg, MD, USA, and AllCells, Foster City, CA, USA). CD34+ cells were selected 1 day
2000Xu, J;Stolk, JA;Zhang, X;Silva, SJ;Houghton, RL;Matsumura, M;Vedvick, TS;Leslie, KB;Badaro, R;Reed, SG;Identification of differentially expressed genes in human prostate cancer using subtraction and microarrayCancer Res.10749139Truncated P503S (amino acid 113–241) was cloned into pET32b (Novagen) and expressed in E. coli as thioredoxin fusion proteins with a histidine tag. P503S was purified by nickel chromatography, digested with thrombin, and further purified by reverse-p
2000Kömüves, LG;Hanley, K;Lefebvre, AM;Man, MQ;Ng, DC;Bikle, DD;Williams, ML;Elias, PM;Auwerx, J;Feingold, KR;Stimulation of PPARalpha promotes epidermal keratinocyte differentiation in vivoJ. Invest. Dermatol.10951268purchased from Vector (Burlingame, CA). Peroxidase activity was revealed with diaminobenzidine (QualTek Laboratories, Santa Barbara, CA), followed by counterstaining with methyl green. Omitting the first antibodies resulted
2000Johnson, LV;Ozaki, S;Staples, MK;Erickson, PA;Anderson, DH;A potential role for immune complex pathogenesis in drusen formationExp. Eye Res.10865992ANDERSONa a Center for the Study of Macular Degeneration, Neuroscience Research Institute, University of California, Santa Barbara, CA 93106, USA, b QualTek Molecular Laboratories, Santa Barbara, CA 93111, USA (Received
2000Yeung, G;Mulero, JJ;McGowan, DW;Bajwa, SS;Ford, JE;CD39L2, a gene encoding a human nucleoside diphosphatase, predominantly expressed in the heartBiochemistry11041856the manufacturer. Automated in situ hybridization was performed by QualTek Molecular labs (Santa Barbara, CA) using a modified version of a previously published procedure (17). The Ventana Medical Systems, Inc. (Tucson
2000Kralchevsky, PA;Nagayama, K;Capillary interactions between particles bound to interfaces, liquid films and biomembranesAdvances in colloid and interface science10768480This dissertation studies cellular rearrangements in tissues and attempts to establish the role of physical properties of cells, tissues and membranes in several biological phenomena. Save
2000Pettus, P;Modeling Fecal Bacteria in Oregon Coastal Streams Using Spatially Explicit Watershed CharacteristicsPortland State UniversityPathogens, such as Escherichia coli and fecal coliforms, are causing the majority of water quality impairments in US, making up∼ 87% of this grouping's violations. Predicting and Save
2000Mostafa, SS;Effects of culture oxygen tension on human megakaryocytes: A phenotypic and mechanistic studyThesisAll of the molecular studies were conducted using CD34+ cells from fresh apheresis products (~ 2% CD34+ cells) collected from G-CSF mobilized normal donors (Poietics Technologies, Gaithersburg, MD and AllCells, Foster City, CA). PB instead of BM cell
1999Hubert, RS;Vivanco, I;Chen, E;Rastegar, S;Leong, K;Mitchell, SC;Madraswala, R;Zhou, Y;Kuo, J;Raitano, AB;Jakobovits, A;Saffran, DC;Afar, DE;STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumorsProc. Natl. Acad. Sci. U.S.A.10588738Human tissues for RNA and protein analyses were obtained from the Human Tissue Resource Center at the University of California, Los Angeles; from the National Disease Research Interchange (Philadelphia); and from QualTek Molecular Labs (Santa Barbara
1999Kömüves, LG;Hanley, K;Jiang, Y;Katagiri, C;Elias, PM;Williams, ML;Feingold, KR;Induction of selected lipid metabolic enzymes and differentiation-linked structural proteins by air exposure in fetal rat skin explantsJ. Invest. Dermatol.10084306purchased from Vector (Burlingame, CA). Peroxidase activity was revealed with DAB substrate (QualTek Laboratories, Santa Barbara, CA) followed by counterstaining with methyl green. Omitting the first antibodies resulted
1999Hu, MC;Wang, YP;Mikhail, A;Qiu, WR;Tan, TH;Murine p38-delta mitogen-activated protein kinase, a developmentally regulated protein kinase that is activated by stress and proinflammatory cytokinesJ. Biol. Chem.10066767From the ‡ Department of Cell Biology, Amgen, Inc., Thousand Oaks, California 91320, ¶ Phylogeny, Inc., Columbus, Ohio 43212, and the ‖ Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030. Next Section. Abstrac
1999Widder, E;Johnsen, S;Bernstein, S;Case, J;Neilson, D;Thin layers of bioluminescent copepods found at density discontinuities in the water columnMarine BiologyCommunicated by NH Marcus, Tallahassee EA Widder (8) a S. Johnsen Harbor Branch Oceanographic Institution, Fort Pierce, Florida 34946, USA Fax: 001 (0)561 468-0757 e-mail: widder@hboi.edu SA Bernstein QualTek, Santa Barbara, California 93111, USA JF
1999Payre, F;Vincent, A;Carreno, S;ovo/svb integrates Wingless and DER pathways to control epidermis differentiationNature10421370Similar results were obtained in allcells of the embryonic ventral epidermis tested, using a variety of Gal4 drivers. The denticle production induced by ectopic svb expression does not Save
1998Weymann, R;Jannuzi, B;Lu, L;Bahcall, J;Bergeron, J;Boksenberg, A;Hartig, G;Kirhakos, S;Sargent, W;Savage, B;Schneider, D;Turnshek, D;Wolfe, A;TheHubble Space TelescopeQuasar Absorption Line Key Project. XIV. The Evolution of Lyα Absorption Lines in the Redshift Intervalz= 0-1.5The Astrophysical JournalWe present the results of an analysis of the rate of evolution of the Lyα absorption lines in the redshift interval 0.0 to~ 1.5 based upon a sample of 987 Lyα absorption lines identified in Save
1997Schmidt-Erfurth, U;Diddens, H;Birngruber, R;Hasan, T;Photodynamic targeting of human retinoblastoma cells using covalent low-density lipoprotein conjugatesBritish journal of cancer9000598Firstly, the apoB/E receptor, which is present on allcells, is the main mechanism by which cells gain exogenous cholesterol and can be regulated up or down to attain cholesterol Save
1997Surinya, KH;Heme biosynthesis in erythroid cells : transcriptional egulation of the human 5=aminolevulinate synthase 2 gene / a thesis submitted to the University of Adelaide for the degree of Doctor of Philosophy by Katharina Helen SurinyaThesisThere are two isozymes for ALAS: a housekeeping form expressed in allcells (ALAS1), and an erythroid-specific form (ALAS2) responsible for the higher protoporphyrin synthesis in Save
1996Kavanagh, K;Whittaker, PA;Application of protoplast fusion to the nonconventional yeastEnzyme and microbial technologyMicrobio/. 1983. 129, 337 IL3377 Necas. 0.. Svoboda, A. and Havelkova, M. Mechanism of regen- eration of yeast protoplasts: Formation of the cell wall in %hixmc- ckcwon~~~~es pornbr. Folio. Bio[. 1968, 14, 80-85 Necan. 0. Cell wall synthesis in yeast
1996Evers, BM;Zhou, Z;Dohlen, V;Rajaraman, S;Thompson, JC;Townsend, CM;Fetal and neoplastic expression of the neurotensin gene in the human colonAnnals of surgery8651737This gene is present in allcells in the body, but, interestingly, it is differentially expressed. As shown in this study, it tends to be expressed in the fetal colon and in the adult small bowel. Save
1996Salinas, E;Abbott, LF;A model of multiplicative neural responses in parietal cortexProceedings of the National Academy of Sciences of the United States of America8876244Thesetwo parameterswill either be the same for allcells, in which case we refer to them as m andb, orwill be chosen from Gaussian distributions with fixed means and standard Save
1996Nwoga, JC;Sniffen, JC;Peña-Rasgado, C;Kimler, VA;Rasgado-Flores, H;Effect of pentachlorophenol on calcium accumulation in barnacle muscle cellsThe Journal of physiology9011605Allcells were mounted in the experimental chamber in the presence of NaSWOCa (see Methods). Except for the case where the cells remained in NaSWOCa, cells were pre-incubated Save
1996Kineman, RD;Gettys, TW;Frawley, LS;Role of guanine nucleotide-binding proteins, Gi alpha 3 and Gs alpha, in dopamine and thyrotropin-releasing hormone signal transduction: evidence for competition and commonalityThe Journal of endocrinology8778223Given that SLO is an invasive agent and could result in cell lysis (approximately 1-2% of allcells lyse following SLO treatment), the RHPA allows for the evaluation ofPRL release from Save
1996Berry, RM;Berg, HC;Torque generated by the bacterial flagellar motor close to stallBiophysical journal8968619
1996Goodman, MB;Art, JJ;Positive feedback by a potassium-selective inward rectifier enhances tuning in vertebrate hair cellsBiophysical journal8804626
1996Laughlin, MJ;Christiansen, NP;Herzig, GP;Blumenson, L;Bonney, D;Stewart, CC;CD34 progenitor cell subset analyses in normal human bone marrow and marrow harvested after intermediate-dose chemotherapyCytometry8979021CD34 + cells exhibiting high side scatter, R8 (to include allcells to theright ofR1)were >95% basophils(Fig. 2f). Most mature basophils exhibit low side scatter because their granules Save
1995Wang, XJ;Golomb, D;Rinzel, J;Emergent spindle oscillations and intermittent burst firing in a thalamic model: specific neuronal mechanismsProceedings of the National Academy of Sciences of the United States of America7777551The histograms were obtained by computing the mean bursting rates of allcells averaged over a timeinterval of 10 s. (D) Normalized interburst interval (IBI) histogram (bin = 10 ms) Save
1995Nooter, K;Boersma, AW;Oostrum, RG;Burger, H;Jochemsen, AG;Stoter, G;Constitutive expression of the c-H-ras oncogene inhibits doxorubicin-induced apoptosis and promotes cell survival in a rhabdomyosarcoma cell lineBritish journal of cancer7880739At 1 JM doxorubicin - a concentration that gives more than 4 log cell kill in a clonogenic assay on the R2NEO cells (Figure 3) - 96 h after drug incubation practically allcells were Save
1995Hofstadler, SA;Swanek, FD;Gale, DC;Ewing, AG;Smith, RD;Capillary electrophoresis-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for direct analysis of cellular proteinsAnalytical chemistry7741217The initial cell population has a significant spatial distribution, and it is unlikely that allcells lysed simultaneously, leading to band dispersion greater than that due to the initial spatial Save
1995Juckett, MB;Balla, J;Balla, G;Jessurun, J;Jacob, HS;Vercellotti, GM;Ferritin protects endothelial cells from oxidized low density lipoprotein in vitroThe American journal of pathology7677189In allcells, ferritin synthesis is tightly coupled with the intracellular iron concentration, which presumably prevents expansion of a potentially damaging free iron pool. We have found that Save
1995Preudhomme, C;Lepelley, P;Lovi, V;Zandecki, M;Cosson, A;Fenaux, P;T-cell acute lymphoblastic leukemia occurring in the course of B cell chronic lymphocytic leukemia: a case reportLeukemia & lymphoma8535207In the six cases studied, similar immunoglobulin chain isotypes were found in the CLL and ALLcells, while identical cytogenetic abnormalities were present in CLL and ALL in the Save
1994Uckun, F;Downing, J;Chelstrom, L;Gunther, R;Ryan, M;Simon, J;Carroll, A;Tuel-Ahlgren, L;Crist, W;Human t(4;11)(q21;q23) acute lymphoblastic leukemia in mice with severe combined immunodeficiencyBlood
1994Shikama, N;Ackermann, R;Brack, C;Protein synthesis elongation factor EF-1 alpha expression and longevity in Drosophila melanogasterProceedings of the National Academy of Sciences of the United States of America8183891Therefore, regulation of protein synthesis can beconsidered as one key homeostatic function common to allcells. A decrease in the rate ofprotein synthesis with advancing age has been Save
1994Yao, A;Rubin, H;A critical test of the role of population density in producing transformationProceedings of the National Academy of Sciences of the United States of America8052648In a competentpopulation, allcells have the capacityto produce some transformed foci if kept under the constraint of confluence and/or reduced serum concentration for extended Save
1994Ingenbleek, Y;Young, V;Transthyretin (prealbumin) in health and disease: nutritional implicationsAnnual review of nutrition7946531the fteld of both thyroid hormones (175, 176), whose three speciftc carrier proteins act as buffering reservoirs, thereby allowing the uniform distribution of the horн mones among allcells Save
1994Ferreira, GC;Mammalian ferrochelatase. Overexpression in Escherichia coli as a soluble protein, purification and characterizationThe Journal of biological chemistry8308010dance of ferrochelatase in mitochondria and its difficulty of Although ferrochelatase is present in allcells, it is predomi- solubilization from the membrane environment (1,2). In this nantly Save
1994McDonnell, DP;Goldman, ME;RU486 exerts antiestrogenic activities through a novel progesterone receptor A form-mediated mechanismThe Journal of biological chemistry8163495In the pri- suggests that estrogen receptor-mediated activation of tranmate uterus, for instance, in addition to functioning an an- scription is not identical in allcells. Since PR-A is not Save
1994Skiba, PJ;Keesler, GA;Tabas, I;Interferon-gamma down-regulates the lipoprotein(a)/apoprotein(a) receptor activity on macrophage foam cells. Evidence for disruption of ligand-induced receptor recycling by interferon-gammaThe Journal of biological chemistry8083207Allcells were then assayed for 5 h 3 (in the absence IFN-y) for lZ5I-r-apo(a) degradation. C,resident mouse peritoneal macrophages werepreincubated for 3 days in DMEM, 10%LPDS, Save
1994Cazenave, C;Frank, P;Toulme, JJ;Büsen, W;Characterization and subcellular localization of ribonuclease H activities from Xenopus laevis oocytesThe Journal of biological chemistry7929207to be primarily concentrated in the germinal drawn from the various attempts to characterize these envesicle, but around of this activity was detected 5% in the zymes further: allcells Save
1994Carrel, F;Dharmawardhane, S;Clark, AM;Powell-Coffman, JA;Firtel, RA;Spatial and temporal expression of the Dictyostelium discoideum G alpha protein subunit G alpha 2: expression of a dominant negative protein inhibits proper prestalk to stalk differentiationMolecular biology of the cell8186466Using the full-length promoter, aggregates showed uniform staining during aggregation and mound formation, suggesting that Ga2 is expressed in allcells at this stage of development, Save
1994Aoyagi, M;Furusawa, S;Waga, K;Tsunogake, S;Shishido, H;Suppression of normal hematopoiesis in acute leukemia: effect of leukemic cells on bone marrow stromal cells and hematopoietic progenitor cellsInternal medicine (Tokyo, Japan)7949632Effects ofleukemic cells (LC) onbone marrowstromal cells and myeloid progenitor cells (CFUC) were studied in vitro, using LClines with different lineage characteristics. LCand/or LC- Save
1994Van Hamme, LJ;Associative and statistical accounts of cue competition in causality judgmentsThesisThis expression equ ally utilizes the inform ation in allcells of the ta b le and re p re sen ts one measure of the tru e contingency between the even ts. Although developed w ith in qu ite Save
1993Kohl, NE;Mosser, SD;deSolms, SJ;Giuliani, EA;Pompliano, DL;Graham, SL;Smith, RL;Scolnick, EM;Oliff, A;Gibbs, JB;Selective inhibition of ras-dependent transformation by a farnesyltransferase inhibitorScience (New York, N.Y.)8316833Apparently, lovastatin's growth-inhibitory effects involve multiple mevalonate-dependent pathways that are required for allcells to growand divide. The specificity observed with L- Save
1993Campana, D;Coustan-Smith, E;Manabe, A;Buschle, M;Raimondi, SC;Behm, FG;Ashmun, R;Aricò, M;Biondi, A;Pui, CH;Prolonged survival of B-lineage acute lymphoblastic leukemia cells is accompanied by overexpression of bcl-2 proteinBlood8427984Overexpression of bcl-2 delays the onset of apoptosis in lymphohematopoietic cells. We measured levels of bcl-2 protein in normal and leukemic human B-cell progenitors with a Save
1993Ruiz-Gómez, M;Ghysen, A;The expression and role of a proneural gene, achaete, in the development of the larval nervous system of DrosophilaThe EMBO journal8458326The late expression is abnormal in that most or allcells of the clusters shift to, and stay at, the high level of expression that is normally observed only in the progenitor cell. We conclude Save
1993Rice, NR;Ernst, MK;In vivo control of NF-kappa B activation by I kappa B alphaThe EMBO journal8223478Whatever the basis for IxBct's instability in the cell lines examined here, there is no apriori reason to think that allcells have such unstable IxBa.For example, if the hypothetical kinase Save
1993Camplejohn, RS;Brock, A;Barnes, DM;Gillett, C;Raikundalia, B;Kreipe, H;Parwaresch, MR;Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cellsBritish journal of cancer7682430There is considerable interest in immunohistochemical markers of proliferation which are suitable for use on routinely fixed clinical material. The novel proliferation-associated antibody Save
1993Art, JJ;Fettiplace, R;Wu, YC;The effects of low calcium on the voltage-dependent conductances involved in tuning of turtle hair cellsThe Journal of physiology8308720Allcells tuned to very low frequencies also possessed an inward rectifier (see records in Fig. C) revealed with hyperpolarizing pulses negativeto the K+ equilibrium potential. An inward Save
1993Cociancich, S;Ghazi, A;Hetru, C;Hoffmann, JA;Letellier, L;Insect defensin, an inducible antibacterial peptide, forms voltage-dependent channels in Micrococcus luteusThe Journal of biological chemistry7690029There are at least two possible explanations for this observation: either allcells are not hit by defensin, or they are all hit, but areonly partially depleted of K'. A partial depletion of K' Save
1993Masumoto, A;Hemler, ME;Multiple activation states of VLA-4. Mechanistic differences between adhesion to CS1/fibronectin and to vascular cell adhesion molecule-1The Journal of biological chemistry7677996We examined the effects of a stimulatory anti-beta 1 mAb (TS2/16) and different divalent cations on VLA-4-mediated cell adhesion to vascular cell adhesion molecule-1 (VCAM-1), to the Save
1993Stauffer, P;Zhao, H;Luby-Phelps, K;Moss, R;Star, R;Muallem, S;Gap junction communication modulates [Ca2+]i oscillations and enzyme secretion in pancreatic acini.The Journal of biological chemistryThese oscillations stopped in allcells on stopping stimulationindependent of the number or the length of the focal stimulations. Global CCKJ stimulation of the same acinus also induced [ Save
1993Sinha, SK;Todd, SC;Hedrick, JA;Speiser, CL;Lambris, JD;Tsoukas, CD;Characterization of the EBV/C3d receptor on the human Jurkat T cell line: evidence for a novel transcriptJournal of immunology (Baltimore, Md. : 1950)8390533(28) demonstrated that HPB-ALLcells coexpress CR2 and CRl and that a portion of these two C3 receptors are associated in the form of CRl/CR2 complexes in the cell membrane. Save
1993Panzeter, PL;Ringer, DP;DNA precursors are channelled to nuclear matrix DNA replication sitesThe Biochemical journal8352746Precursors injected into the bloodstream do not have equal access to allcells, especially during the short pulse times used in this study. Many replicating cells will not see the precursor Save
1993D'Andrea, RJ;Stratmann, R;Lehner, CF;John, UP;Saint, R;The three rows gene of Drosophila melanogaster encodes a novel protein that is required for chromosome disjunction during mitosisMolecular biology of the cell8305737(I) An embryo during cellularization showing lack of transcripts in allcells except pole (presumptive germ) cells. 0) Cellular blastodern at higher magnification showingpunctate staining Save
1993Dorocicz, IR;Williams, PM;Redfield, RJ;The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competenceJournal of bacteriology8226661This cAMP-independent event must be induced in allcells when cultures are transferred to MIV, but it may also occur spontaneously in the small fraction of cells that develop Save
1993Humm, J;Chin, L;A Model of Cell Inactivation by Alpha-Particle Internal EmittersRadiation ResearchThe bottom survival curve is produced by 50% of the sources bound uniformly to allcells. The middle curve is for 50% of the source decays occurring on half of the cells for type A Save
1993Mukhtar, DD;Stewart, IJ;Observations of the surface morphology of cultured granulated metrial gland cellsJournal of anatomy8226296The features described above were found on GMG cells at all stages of culture examined and on allcells from all stages of pregnancy examined. However, it was noticeable that the Save
1993Edelsbrunner, H;Seidel, R;Sharir, M;On the Zone Theorem for Hyperplane ArrangementsSIAM Journal on ComputingWe define Zone(b; H) to be the set of allcells in the arrangement A(H) that intersect the hypewlane b, and the cells were defined to be open sets. Previously the zone used to be defined Save
1993Ohno, H;Inoue, T;Akasaka, T;Okumura, A;Miyanishi, S;Ohashi, I;Kikuchi, M;Masuya, M;Amano, H;Imanaka, T;Acute lymphoblastic leukemia associated with a t(1;19)(q23;p13) in an adultInternal medicine (Tokyo, Japan)8286840ThePCRanalysis of the present patient revealed the samemolecular evidence of E2A/PBX1 fusion as found in childhood ALLcells. Thus, molecular analysis of the E2A/PBX1fusion gene Save
1993Herrmann, H;Karsch, F;LARGE SCALE COMPUTATIONAL PHYSICS ON MASSIVELY PARALLEL COMPUTERSLarge Scale Computational Physics on Massively Parallel Computerstime matrix vector multiplication 19.4 sec sum over allcells 0.012 sec Time for allcells to allcells communication. Save
1993Shamim, KM;Frog retinal ganglion cells. Morphological and spatial analysesThesisFor an auto-correlogram, allcells of the same type as the central reference cell are picked up (asshown schematically in A3) while a cross-correlogram locates all cellsof another type in Save
1992Kim, CY;Giaccia, AJ;Strulovici, B;Brown, JM;Differential expression of protein kinase C epsilon protein in lung cancer cell lines by ionising radiationBritish journal of cancer1329908The effect of ionising radiation on the regulation of gene and protein expression is complex. This study focuses on the translational regulational of the epsilon isoform of protein kinase C Save
1992Rooney, IA;Davies, A;Morgan, BP;Membrane attack complex (MAC)-mediated damage to spermatozoa: protection of the cells by the presence on their membranes of MAC inhibitory proteinsImmunology1374057On fluorescence microscopy, allcells stained brightly with both BRIC 229 and A 35 anti-CD59 antibodies (Fig. 2a-c). The pattern ofstaining vfaried (eg Fig. Save
1992Smedts, F;Ramaekers, F;Troyanovsky, S;Pruszczynski, M;Robben, H;Lane, B;Leigh, I;Plantema, F;Vooijs, P;Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasiaThe American journal of pathology1372156A completely filled bar indicates an intensely positive reaction in allcells. An open bar indicates that all cells are negative, whereas a small square indicates that only afew cells Save
1992Hohmann, HP;Remy, R;Aigner, L;Brockhaus, M;van Loon, AP;Protein kinases negatively affect nuclear factor-kappa B activation by tumor necrosis factor-alpha at two different stages in promyelocytic HL60 cellsThe Journal of biological chemistry1730737Allcells were used after reaching near confluence or a density of 1-1.5 X lo6 cells/ml of culture medium. Preparation of Nuclear Extracts and Electrophoretic Mobility Shift Assays (EMSA) Save
1992Kirby, MS;Sagara, Y;Gaa, S;Inesi, G;Lederer, WJ;Rogers, TB;Thapsigargin inhibits contraction and Ca2+ transient in cardiac cells by specific inhibition of the sarcoplasmic reticulum Ca2+ pumpThe Journal of biological chemistry1320000Recently, it has been reported that in allcells examined, a common response evoked by thapsigargin is a rapid increase in cytosolic [Ca2+],possibly resulting from the mobilization of Save
1992Bednarczyk, J;Szabo, M;McIntyre, B;Post-translational processing of the leukocyte integrin alpha 4 beta 1.Journal of Biological ChemistryEndo F digestion of a4 and B1 from HPB-ALLcells. and and IL-2-dependent T lymphocytes cultured for 43 days (Fig. HPB-ALL cells were 'ZsII-cell-surface-labeled lysed in Nonidet P4). Save
1992García-Alvarado, JS;Rodriguez, MA;Labbé, RG;Influence of elevated temperature on starch hydrolysis by enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens type AApplied and environmental microbiology1371660The suspension was sonicated for 45 s with 60-s cooling periods until essentially allcells were disrupted. The sample was thencentrifuged at 10,000 x g for 30 min, and Save
1992Szabà, G;Pine, PS;Weaver, JL;Kasari, M;Aszalos, A;Epitope mapping by photobleaching fluorescence resonance energy transfer measurements using a laser scanning microscope systemBiophysical journal1380319
1992Shatz, CJ;The developing brainScientific American26269896Allcells on the array fired with in about five seconds of one another, in a predictable and rhythmic pattern. The bursts of action potentials lasted several seconds and were followed by Save
1991Bejar, I;A GENERATIVE APPROACH TO PSYCHOLOGICAL AND EDUCATIONAL MEASUREMENTETS Research Report Seriesin this taxonomy could be assessed by the degree to which the psychometric parameters from a given cell are well-predicted and the within-cell residuais are constant across allcells. Save
1991Burton, GF;Kosco, MH;Szakal, AK;Tew, JG;Iccosomes and the secondary antibody responseImmunology1879876The resulting preparations were observed microscopically to ensure that allcells were disrupted. Antigen concentration was determined by using radiolabelled antigen and assessing Save
1991Hudson, TH;Grillo, FG;Brefeldin-A enhancement of ricin A-chain immunotoxins and blockade of intact ricin, modeccin, and abrinThe Journal of biological chemistry1917980This receptor interacts with transport of newly synthesized plasma membrane glycoproproteoglycans in allcells studied the Gal bindingsitesontheB-chain of ricin and would teins, Save
1991Novy, RE;Lin, JL;Lin, JJ;Characterization of cDNA clones encoding a human fibroblast caldesmon isoform and analysis of caldesmon expression in normal and transformed cellsThe Journal of biological chemistry1885618Allcells were grownin Dulbecco's modified Eagle's mediumcontaining 10% fetal calf serum and maintained in a 37 "C humidified incubator with 95% air and 5% CO,. Western Blot and Save
1991Järveläinen, HT;Kinsella, MG;Wight, TN;Sandell, LJ;Differential expression of small chondroitin/dermatan sulfate proteoglycans, PG-I/biglycan and PG-II/decorin, by vascular smooth muscle and endothelial cells in cultureThe Journal of biological chemistry1744124
1991van Praag, MC;Boom, BW;van Hees, CL;Vermeer, BJ;Scheffer, E;Classical and ulcerative lichen planus with plasma cell infiltrateArchives of dermatology1990998Plasma cells accounted for 60% to 70% of allcells. The infiltrate invaded the epidermis causing apoptoticand vacuolar degeneration of basal and parabasal cells (Fig 2). The lesions of Save
1991Humbert, P;Agache, P;Acquired ichthyosis: a new cutaneous marker of autoimmunityArchives of dermatology1990997Plasma cells accounted for 60% to 70% of allcells. The infiltrate invaded the epidermis causing apoptoticand vacuolar degeneration of basal and parabasal cells (Fig 2). The lesions of Save
1991Hall, AJ;Steady size distributions in cell populations : a thesis presented in partial fulfilment of the requirements for the degree of Doctor Philosophy in Mathematics at Massey UniversityThesisSpecial cases are also considered where allcells with the same birth-size divide at the ), and allcells divide into equal-sized daughters, then we obtain a functional differential equation. Save
1990Finn, PW;Kara, CJ;Van, TT;Douhan, J;Boothby, MR;Glimcher, LH;The presence of a DNA binding complex correlates with E beta class II MHC gene expressionThe EMBO journal2328725Even though the three motifs do not enhance CAT activity when tested individually, allcells that we have tested contain factors that bind to W, X and Y (Figure 4). Save
1990Yayon, A;Klagsbrun, M;Autocrine transformation by chimeric signal peptide-basic fibroblast growth factor: reversal by suraminProceedings of the National Academy of Sciences of the United States of America2164679Most if not allcells possess FGF receptors, which are phosphorylated andinternalized upon binding of FGF. A 130-kDa high-affinity FGF receptor containing an Save
1990Berger, JD;Rahemtullah, S;Commitment to autogamy in Paramecium blocks mating reactivity: implications for regulation of the sexual pathway and the breeding systemExperimental cell research2298253From this point on, allcells become committed to autogamy at a cell density that is lover than that required for the onset of mating reactivity. The cell cycle experiments discussed above Save
1990Dixon, CJ;Woods, NM;Cuthbertson, KS;Cobbold, PH;Evidence for two Ca2(+)-mobilizing purinoceptors on rat hepatocytesThe Biochemical journal2386488Other than in the total absence of receptors of both subsets, allcells will respond to ATP.We propose that the cells responding to ATP with transients of short duration lack receptors of Save
1989Malinow, R;Schulman, H;Tsien, RW;Inhibition of postsynaptic PKC or CaMKII blocks induction but not expression of LTPScience (New York, N.Y.)2549638Bath-applied H-7 can suppress the expression of LTP (3), although it is not known whether the locus of action is pre- or postsynaptic, since it has access to allcells in the preparation. To Save
1989Johnson, JP;Stade, BG;Holzmann, B;Schwäble, W;Riethmüller, G;De novo expression of intercellular-adhesion molecule 1 in melanoma correlates with increased risk of metastasisProceedings of the National Academy of Sciences of the United States of America2643120Identical restriction fragments were observed in allcells analyzed, including four autologous melanoma/B-cell pairs. The P3.58/ICAM-1 gene, therefore, does not seem to be polymorphic Save
1989Knust, B;Brüggemann, U;Doerfler, W;Reactivation of a methylation-silenced gene in adenovirus-transformed cells by 5-azacytidine or by E1A trans activationJournal of virology2473219Of course, it was not possible to show that allcells had been transfected equally efficiently. Reactivation of E2Atranscription might have occurred only in a subpopulation of the HE3 cells. Save
1989Celis, E;Karr, RW;Presentation of an immunodominant T-cell epitope of hepatitis B surface antigen by the HLA-DPw4 moleculeJournal of virology2463381Thus, it appears that in the heterogeneous PBMC population not allcells express DPw4 molecules and that only thosethat do can present HBsAg (or Sld) to the Th. The inhibitory effect Save
1989Navaratnam, V;Woodward, JM;Skepper, JN;Specific heart granules and natriuretic peptide in the developing myocardium of fetal and neonatal rats and hamstersJournal of anatomy2532637Within the fetal atria, almost allcells seem capable of ANP synthesis and the concentration of ANP immunoreactivity increases sharply with development even after birth. Recent light Save
1988Nicolson, GL;Dulski, KM;Trosko, JE;Loss of intercellular junctional communication correlates with metastatic potential in mammary adenocarcinoma cellsProceedings of the National Academy of Sciences of the United States of America3422438, and the gene coding for polyoma virus middlesized tumor (middle T) antigen can decrease intercellular junctional communication in certain cellsystems (13-16), although not allcells Save
1988Hall, JG;Review and hypotheses: somatic mosaicism: observations related to clinical geneticsAmerican journal of human genetics3052049Conditions such as McCune-Albright syndrome may well represent lethal human mutations when present in allcells, but, by isolating the tissue involved, it may be possible to compare Save
1988Siebenlist, U;Bressler, P;Kelly, K;Two distinct mechanisms of transcriptional control operate on c-myc during differentiation of HL60 cellsMolecular and cellular biology3162550These sites occur in allcells which we have analyzed (46, 47). In previous reports (3, 20, 22, 28, 29), only partial and even somewhat conflicting information on the chromatin structure of Save
1988Adány, R;Glukhova, MA;Kabakov, AY;Muszbek, L;Characterisation of connective tissue cells containing factor XIII subunit aJournal of clinical pathology2893813Though the expression of certain antigens might be lost at culture, allcells stained for the fibroblast markers IIIG1O and alkaline phosphatase lack this antigen. This clearly suggests that Save
1988Kim, T;Zeigler, B;The class kernel-models in DEVS-scheme: a hypercube architecture exampleACM SIGSIM Simulation Digestset-allcells-positions, which generates 2 n number of n-tuple coordinates to be attached to corresponding components before locating them in their positions. The method set-allcells- Save
1988Reno, PW;Immunochemical Approaches to Coastal, Estuarine and Oceanographic QuestionsCoastal and EstuarineN ear ly identic al reactions were found in kidney (Figure 2?) and liver (data not shown) -allcells seeme d to conta in IPN antige n. However, gastrointestin al tra ct tissue, especiallyt he Save
1987Art, JJ;Fettiplace, R;Variation of membrane properties in hair cells isolated from the turtle cochleaThe Journal of physiology2443666The shape of the current-voltage relationship was found to be similar in allcells, being flat at hyperpolarized levels and increasing its slope with depolarization through the resting Save
1987Segarini, PR;Roberts, AB;Rosen, DM;Seyedin, SM;Membrane binding characteristics of two forms of transforming growth factor-betaThe Journal of biological chemistry2889729Essentially allcells have high affinity receptors that specifically bind TGF-/3 (Kdvalues of 1-60 pM, Refs. 11, 12, 14, and 15). The receptor number is tissue- and species-specific and Save
1987Richardson, A;Butler, JA;Rutherford, MS;Semsei, I;Gu, MZ;Fernandes, G;Chiang, WH;Effect of age and dietary restriction on the expression of alpha 2u-globulinThe Journal of biological chemistry2442168regulation of gene expression represents one site regulation of common to allcells in a living organism, and changes in gene 24. expression can have profound effect at both the a Save
1987ROBERTS, D;A zonal method for modeling 3-D aircraft flow fields with jet plume effects19th AIAA, Fluid Dynamics, Plasma Dynamics, and Lasers ConferenceCON is then updated, and the cycle 18 repeated until the error in continuity falls below a prescribed tolerance for allcells . The relaxation factor, 0, Save
1986Greaves, MF;Differentiation-linked leukemogenesis in lymphocytesScience (New York, N.Y.)3535067us the monodonal antibody-defined makers T9 and T10, described initially on T-ALLcells and normal thymocyte subses (21), are not T lineage or maturation stage restrictedbut are Save
1986Mann, GJ;Fox, RM;Deoxyadenosine triphosphate as a mediator of deoxyguanosine toxicity in cultured T lymphoblastsThe Journal of clinical investigation3490493Allcells were maintained at 37C in suspension culture in Hepes-buffered Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% fetal bovine serum, glutamine (2 Save
1986Slater, LM;Sweet, P;Stupecky, M;Gupta, S;Cyclosporin A reverses vincristine and daunorubicin resistance in acute lymphatic leukemia in vitroThe Journal of clinical investigation3457021It has recently been reported that human ALLcells have a 10-fold greater concentration of calmodulin than normal peripheral blood lymphocytes and that CsA is a potentinhibitor of T Save
1986Cowley, GP;Smith, JA;Gusterson, BA;Increased EGF receptors on human squamous carcinoma cell linesBritish journal of cancer2420349The binding kinetics for allcells examined showed the typical pattern of a high and low affinity receptor with dissociation constants of 10-10M and 1-9M respectively. Recent Save
1986Stewart, MA;Warnock, M;Wheeler, A;Wilkie, N;Mullins, JI;Onions, DE;Neil, JC;Nucleotide sequences of a feline leukemia virus subgroup A envelope gene and long terminal repeat and evidence for the recombinational origin of subgroup B virusesJournal of virology3009890FEA cells (21) were infected in mass culture with various FeLV isolates and passaged for 3 weeks to allow virus spread to allcells. DNA was purified from these cell cultures for use in Save
1986DiFrancesco, D;Ferroni, A;Mazzanti, M;Tromba, C;Properties of the hyperpolarizing-activated current (if) in cells isolated from the rabbit sino-atrial nodeThe Journal of physiology2432247The reason for this discrepancy may be the current run-down that occurs, although to a highly variable degree, in allcells. This feature of if is treated insome detail below. Here it may be Save
1986Hartman, BJ;Tomasz, A;Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureusAntimicrobial agents and chemotherapy3488015explanation of heterogeneity is that allcells in the heterogeneous culture carry the genetic marker(s) of methicillin resistance, but for some reason the phenotypic expression of Save
1986Wang, CY;Bushkin, Y;Pica, R;Lane, C;McGrath, H;Posnett, DN;Stimulation and expansion of a human T-cell subpopulation by a monoclonal antibody to T-cell receptor moleculeHybridoma2429909Both the aand (3-chains from normal PBL-T blasts resolved in the pi range of 5.5-6.5, whereas the a- and /3-chains from HPB-ALLcells had acidic and basic pis of 4.0 and 7.0-8.5, Save
1986Steiness, E;Positive inotropic drugs--digitalisActa medica Scandinavica. Supplementum3017057Digitalis is known to inhibit the Na+K+-ATPase in allcells in nearly all species. It has been aqyed that this effect induces increase of the inuacellular sodium concentnfion within the Save
1985Lehrer, RI;Daher, K;Ganz, T;Selsted, ME;Direct inactivation of viruses by MCP-1 and MCP-2, natural peptide antibiotics from rabbit leukocytesJournal of virology2985808Allcells used in this manner were obtained from the American Type Culture Collection except for MDBK cells, which were a gift from Debi Nayak. The virus-infected cell monolayers Save
1985Morris, CS;Hitchcock, E;Immunocytochemistry of folliculo-stellate cells of normal and neoplastic human pituitary glandJournal of clinical pathology3889065folliculo-stellate cells stain for GFAP and S100, although not allcells stained for S100 are GFAP positive. Cocchia and Miani" showed S100 protein within the stellate cells of the pars Save
1985Neyses, L;Locher, R;Stimpel, M;Streuli, R;Vetter, W;Stereospecific modulation of the calcium channel in human erythrocytes by cholesterol and its oxidized derivativesThe Biochemical journal2581557Furthermore, allcells were energized to the same extent by ATP (about 1.7mM). Since the anion channel may be influenced by cholesterol (Schubert & Boss, 1982), we checked Save
1985Hueston, WD;Herd summaries of individual cow somatic cell counts and mastitis control researchThesisand th e re fo re abnormal; 2) allcells in milk were desquamated se c re to ry cells of epithelialo rig in , and th e re fo re a normal p art of la c to genes is ; and 3) the cells in normal milk Save
1984Buick, RN;Pollak, MN;Perspectives on clonogenic tumor cells, stem cells, and oncogenesCancer research6386145In prokaryotes, control of proliferation is unrelated to the process of differentiation, and allcells have proliferative potential. As we have shown, this is not the case in tissues of higher Save
1984Gaukroger, JM;Wilson, L;Protection of cells from methotrexate toxicity by 7-hydroxymethotrexateBritish journal of cancer6590087Cell growth survival studies have revealed that 7-OH methotrexate is two orders of magnitude less cytotoxic to human melanoma and human acute lymphoblastic leukaemia (ALL) cells Save
1984Gussander, E;Adams, A;Electron microscopic evidence for replication of circular Epstein-Barr virus genomes in latently infected Raji cellsJournal of virology6092676Circular forms, which can be isolated either in the covalently closed, supercoiled structure or as a relaxed, open DNA circle (16), have subsequently been found in allcells with more Save
1984Epstein, LM;Forney, JD;Mendelian and non-mendelian mutations affecting surface antigen expression in Paramecium tetraureliaMolecular and cellular biology6092921A genetic and molecular analysis of this mutant suggests that although the A gene is present in the micronucleus of allcells, it is lost during the formation of the macronucleus in the Save
1984Yinon, U;Myopia induction in animals following alteration of the visual input during development: a reviewCurrent eye research6713963For example, asfar as has been determined, allcells of the salamander are capable of ded if fe rent iat ion with appropriate stimulation. Extensive in vitro experimentation on amphibian Save
1984Nelson, DO;Graham, CA;Effects of development and captopril treatment on central angiotensin II--sensitive neuronsClinical and experimental hypertension. Part A, Theory and practice6398145Nearly allcells examined, including neurons which were relatively insensitive to A11 or saralasin, were potently ex
1983Overath, P;Czichos, J;Stock, U;Nonnengaesser, C;Repression of glycoprotein synthesis and release of surface coat during transformation of Trypanosoma bruceiThe EMBO journal6688990Six hours after transfer of the cells to medium SDM-79, ie, at a time when VSG synthesis has already ceased, allcells retain the coat (compare sample at t= 6 h with freshly harvested Save
1983Rowin, KS;Tanowitz, HB;Wittner, M;Nguyen, HT;Nadal-Ginard, B;Inhibition of muscle differentiation by trypanosoma cruziProceedings of the National Academy of Sciences of the United States of America6413976Normal quantities of 13 and y-actin were present in allcells. (B) Rehybridization of the RNA blot shown in A with 32P-labeled DNA withnonmuscle-specific sequencescoding Save
1983Meo, T;Gramsch, C;Inan, R;Höllt, V;Weber, E;Herz, A;Riethmüller, G;Monoclonal antibody to the message sequence Tyr-Gly-Gly-Phe of opioid peptides exhibits the specificity requirements of mammalian opioid receptorsProceedings of the National Academy of Sciences of the United States of America6191329Allcells in the intermediate lobe of rat pituitary were intensely stained (Fig. 3). Higher magnification revealed heavy cytoplasmic localization of the immunoreactive peptides. Save
1983Otteskog, P;Sundqvist, KG;Anchorage and lymphocyte function. Spreading-capacity distinguishes common thymocytes and peripheral T lymphocytesImmunology6339376T-enriched human blood lymphocytes with an adhesive surface in the presence of Concanavalin A (Con A) almost immediately induced a sequence of motilechanges in virtually allcells Save
1983O'NEIL, PM;Log-linear models for evaluating hunting demandThesisBoth the Pearson ch i-sq ua re statistic and th e lik e lih oodratio sum over allcells in th e ta b le . Both a re asymp to tic a lly d is tribu te d as chi - square . Save
1983Burman, LG;Raichler, J;Park, JT;Evidence for diffuse growth of the cylindrical portion of the Escherichia coli murein sacculusJournal of bacteriology6350274The lengths of allcells that contained grains and the distances of the grains from the nearest cell pole were measured with a ruler. Forplotting, these distances were expressed as Save
1982Marx, JL;Cloning the genes of the MHCScience (New York, N.Y.)7071587These regions code not for transplantation antigens, which are expressed on allcells, but for differentiation antigens that are foundonly on certain types of immune cells. The differ Save
1982Zingg, HH;Patel, YC;Biosynthesis of immunoreactive somatostatin by hypothalamic neurons in cultureThe Journal of clinical investigation7130395Immediately after being plated, allcells exhibited a uniform round-shaped appearance and were evenly distributed on the plate surface. After day 5 in culture, many cells showed a Save
1982Donta, ST;Sullivan, N;Wilkins, TD;Differential effects of Clostridium difficile toxins on tissue-cultured cellsJournal of clinical microbiology7107845The two toxins' effects are not similar on allcells, however, since they produce different Allcells were tested at the same time with identical toxin preparations. b ++, All cells rounded Save
1982Ueda, R;Tanimoto, M;Takahashi, T;Ogata, S;Nishida, K;Namikawa, R;Nishizuka, Y;Ota, K;Serological analysis of cell surface antigens of null cell acute lymphocytic leukemia by mouse monoclonal antibodiesProceedings of the National Academy of Sciences of the United States of America6956869Production of Hybridomas Against063 Null-ALLCells. ALL cells wereobtained fromtheperipheralblood ofa22-yearold female patient. The surface phenotypeof the leukemia cells Save
1982Cochrane, DE;Distel, DL;Association of 45calcium with rat mast cells stimulated by 48/80: effects of inactivation, calcium and metabolic inhibitionThe Journal of physiology6816921Mast cells were stimulated by48/80 (1 4g/ml.) and unlabelled Ca (1 mM) added to allcells 10 sec later. Atthe indicated times afterward, 4"Ca (50 #sC/ml.)was added and the incubation Save
1982Ayres, K;Sklar, R;Larson, K;Lindgren, V;Strauss, B;Regulation of the capacity for O6-methylguanine removal from DNA in human lymphoblastoid cells studied by cell hybridizationMolecular and cellular biology6897101so that the dose shown is that which would be observed if allcells had the reactivity of ROT-5. Symbols: A, ROT-5; 0, L6; O, hybrid clone no. 2; E, hybrid clone no. Save
1982Little, LM;Shadduck, JA;Pathogenesis of rotavirus infection in miceInfection and immunity6292110It should be appreciated that in the current study not allcells within any anatomical region fluoresced. Rather, fluorescing cells normally occupied less than 10%, up to perhaps 50%, of Save
1982山田和義, ;岡部直衛, ;宇塚善郎, ;FAB 分類における成人急性白血病の走査電顕及び表面マーカーによる解析臨床血液Almost allcells have ridge-like pro丘1es.(×5,800) Almost allcells are positive for both ANE and CAEin the same cell. b:Almost allcells have ridge-1ike pro丘Ies or microvilli・(×3,000) C: Save
1981Abbott, A;¡Graphs, Not Punctuation!ScienceIndeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save
1981Libet, B;" Human Life" TestimonyScience7244627Indeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save
1981Osborn, EF;Hubbert's EstimatesScience (New York, N.Y.)17782756Indeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save
1981Hamp, EP;;Graphs, not punctuation!Science (New York, N.Y.)17782755Indeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save
1981Engleman, EG;Warnke, R;Fox, RI;Dilley, J;Benike, CJ;Levy, R;Studies of a human T lymphocyte antigen recognized by a monoclonal antibodyProceedings of the National Academy of Sciences of the United States of America7015346A second screening identified a subgroup of these clones whose antibody products bound equally to normal peripheralTcells andto the immunizing T-ALLcells. The binding specificity of Save
1981Yokoyama, K;Stockert, E;Old, LJ;Nathenson, SG;Structural comparisons of TL antigens derived from normal and leukemia cells of Tl+ and TL- strains and relationship to genetically linked H-2 major histocompatibility complex productsProceedings of the National Academy of Sciences of the United States of America6947273H-2 antigens are expressedon virtually allcells ofthe body, whereas TL antigensare normally restrictedto thymocytes in TL+ strains. In contrast to the remarkable polymorphism of H-2 (10 Save
1981Hanania, N;Shaool, D;Poncy, C;Harel, J;Manifold expression of new cellular genes in human lymphoid neoplasiaProceedings of the National Academy of Sciences of the United States of America6947241Previous studies performed in our laboratory have shown that a minor single-stranded DNA component (ssDNA) isolated from the bulk of double-stranded nuclear DNA (nDNA) of Save
1981Mechler, B;Rabbitts, TH;Membrane-bound ribosomes of myeloma cells. IV. mRNA complexity of free and membrane-bound polysomesThe Journal of cell biology6782109Allcells were harvested during exponentialgrowth from cultures not exceeding 5.0 x 106 cells/ml. When labeling was required, [ 3H]uridine, [3Madenosine, or [ 3H]oleic acid wasadded Save
1981Knipe, DM;Batterson, W;Nosal, C;Roizman, B;Buchan, A;Molecular genetics of herpes simplex virus. VI. Characterization of a temperature-sensitive mutant defective in the expression of all early viral gene productsJournal of virology6264126However, the yield of syn+ virus was approximately 10% of the yield of the syn virus, even though allcells were coinfected with both. Therefore, even the wild-type parent of tsB7 could Save
1981Grover, NB;Margalit, A;Zaritsky, A;Ben-Hur, E;Hansen, MT;Sensitivity of exponentially growing populations of Escherichia coli to photo-induced psoralen-DNA interstrand crosslinksBiophysical journal7023563
1981Zaritsky, A;Macnab, RM;Effects of lipophilic cations on motility and other physiological properties of Bacillus subtilisJournal of bacteriology6792185The effects of TPAs+ on the motor were readily reversible: 20 min after treatment with 50 ,uM TPAs+, cells were filtered, washed, and suspended in chemotaxis buffer; 3 minlater, allcells Save
1981Lindgren, V;The location of chromosome breaks in Bloom's syndromeCytogenetics and cell genetics7471822Average of samples IB and 2 only; when allcells are included, the average is 50.7%. Table II. Distribution of breaks in late-non-late-replicating areas1 Total Save
1981Carpio, M;Bellamy, JE;Babiuk, LA;Comparative virulence of different bovine rotavirus isolatesCanadian journal of comparative medicine : Revue canadienne de medecine comparee6268264Twenty-four hours postinfection, when allcells were detached from the monolayers, the entire culture fluids were sonicated to release intracellular virus. The virus was pelleted through a Save
1981Thaller, RA;Potential use of 52FE porphyrins as tumor scanning agentsThesisHowever in vivo tumor killing was not complete resulting in many smaller tumors due to the factthat not allcells were exposed to the light or took up the hematoporphyrin. (220) Over 300 Save
1980O'Lague, PH;Huttner, SL;Physiological and morphological studies of rat pheochromocytoma cells (PC12) chemically fused and grown in cultureProceedings of the National Academy of Sciences of the United States of America6929516In allcells regardless of growth conditions membrane resistance decreased with depolarization. Fig. 1C shows superimposed voltage responses to one depolarizing and one hyperpo Save
1980Lasky, LA;Lev, Z;Xin, JH;Britten, RJ;Davidson, EH;Messenger RNA prevalence in sea urchin embryos measured with cloned cDNAsProceedings of the National Academy of Sciences of the United States of America6254074per average cell given on the lower abscissas are minimal estimates calculated on the basis that there are 600 cells per gastrula and 1500 cells per pluteus embryo and that allcells are Save
1980Haas, M;Patch, V;Genomic masking and rescue of dual-tropic murine leukemia viruses: role of pseudotype virions in viral lymphomagenesisJournal of virology6252327Allcells were grown in Dulbecco-Vogt modified Eagle minimum essential medium supplemented with 10%o fetal bovine serum. All cells were split routinelyat a ratio of 1:16 upon Save
1980Weller, SK;Joy, AE;Temin, HM;Correlation between cell killing and massive second-round superinfection by members of some subgroups of avian leukosis virusJournal of virology6245246this study were determined by interference assays as follows: chicken cells in 100-mm petri dishes were infected with different avian leukosis viruses and passaged twice so that allcells Save
1980Berg, JM;Gardner, HA;Gardner, RJ;Goh, EG;Markovic, VD;Simpson, NE;Worton, RG;Dic(21;21) in a Down's syndrome child with an unusual chromosome 9 variant in the motherJournal of medical genetics6445984In allcells analysed, the translocation chromosome was of the same relative size and banding pattern, indicating a high degree of stability. No short structures or chromosome fragments Save
1980Gerrard, JM;Clawson, CC;White, JG;Lysophosphatidic acids: III. Enhancement of neutrophil chemotaxisThe American journal of pathology7416233Figure 2-When 0.35 ,ug FMP/ml was added to the incubation media used in Figure 1, marked changes were seen in allcells. There were large projections from the body of the cell, and Save
1980Brown, AT;Breeding, LC;Carbon dioxide metabolism by Actinomyces viscosus: pathways for succinate and aspartate productionInfection and immunity6769822Allcells were washed once with 0.01 M potassium phosphate buffer, pH 7.0, and the cell pellets were frozen at -20'C until used. Laboratory cultures of A. viscosus were stored as Save
1980Koppes, LJ;Nanninga, N;Positive correlation between size at initiation of chromosome replication in Escherichia coli and size at initiation of cell constrictionJournal of bacteriology6995452We have also assumed equal growth rate constants for allcells. Variation of the growth rate will tend to decrease the correlation. However, the CV of the specific growth rate is probably Save
1980Ho, CC;Interaction studies of ethanol and psychotropic drugs in rodents: possible involvement of adenosine triphosphatasesThesisD evelopm ent of th e Sodium Pump C oncept T here is ad iffe re nce in io n ic concen tra tio n betw een intracellular and ex tr ace llu la rf lu id s in allcells . Save
1979Detwiler, PB;Hodgkin, AL;Electrical coupling between cones in turtle retinaThe Journal of physiology225478If it is assumed that allcells have the same total membrane resistance, rm, but that different pairs differ in the amount of coupling, then this method of plotting should bring all the points Save
1978Mukherjee, AB;Orloff, S;Butler, JD;Triche, T;Lalley, P;Schulman, JD;Entrapment of metaphase chromosomes into phospholipid vesicles (lipochromosomes): carrier potential in gene transferProceedings of the National Academy of Sciences of the United States of America349565Allcells were checked for mycoplasma (10) and foundto be free of those pathogens. These hybrid cells contributed the metaphase chromosomes which were used to prepare the Save
1978Esber, EC;Movassaghi, N;Leikin, SL;Surface membrane determinants on childhood acute lymphocytic leukemia cells: immunoglobulin, Fc and C3 receptorsClinical and experimental immunology278686Surface membrane determinants on ALLcells Other investigators have found Fc receptors on ALLcells, in combination with either E-rosette formation or with C3 receptors (Haegert et al., Save
1977Thoene, JG;Oshima, RG;Ritchie, DG;Schneider, JA;Cystinotic fibroblasts accumulate cystine from intracellular protein degradationProceedings of the National Academy of Sciences of the United States of America270698After 20 hr, allcells were depleted of intracellular cystine bytreatment with MEA for 1 hr,and then placed in cystine-deficientmedium. Duplicate plates were harvested-at the times shown Save
1977Basset, F;Soler, P;Jaurand, MC;Bignon, J;Ultrastructural examination of broncho-alveolar lavage for diagnosis of pulmonary histiocytosis X: Preliminary report on 4 casesThorax302040and 2) in great numbers in case t, amounting to 7% of allcells examined. In the three other patients, HX cells were much less numerous, but their presence was still diagnostic. In all four Save
1977Genther, CS;Schoeny, RS;Loper, JC;Smith, CC;Mutagenic studies of folic acid antagonistsAntimicrobial agents and chemotherapy329758Since the folic acid pathway is common to allcells, the mechanisms operative with S. faecium will probably be similar in other types of organismssusceptible to these compounds. When Save
1977Rubenstein, M;The effect of salicylate on macromolecular synthesis in mammalian and bacterial cells.ThesisThe inhibition obtained in the growth experiment applied to allcells involved and did not select for a small population of cellswhile drastically inhibiting the remainder. Save
1977Tso-Kai Liu, ;Synthesis of Multilevel Feed-Forward MOS NetworksIEEE Transactions on ComputersTheorem 1: There always exists a feed-forward MOS network with a minimum number ofcells, realizing a given function f such that the output functions of allcells in the network are Save
1976Bootello, A;Fernandez-Cruz, E;Escartin, P;Blanco, MF;Gosalvez, M;Segovia De Arana, JM;Injurious effect on rat liver mitochondria by lymphocytes from patients with primary biliary cirrhosisClinical and experimental immunology1277585The antibody is non-organ/non-species-specific and reacts with allcells containing mitochondria. The antigen is a lipoprotein localized in the inner mitochondrial membranes (Berg et al. Save
1976Gilligan, T;Patterson, A;High field conductance of some acids, bases, and saltsThe Journal of Physical ChemistryParticular care was taken to determine and follow the cell constants of allcells by the method of Jones and Bradshaw.11 It has been found that the cell constants of cells made with Save
1976Malek, KW;A METHOD FOR THE CULTIVATION OF DISPERSED EPITHELIAL CELLS FROM MOUSE PALATAL MUCOSA AND THE EFFECTS OF X-IRRADIATION.Thesislted in the release of cells from the ba sa l layer and a ls o from the lower layers of the stratum spinosum, whereas 30 minutes of incubation resulted in the d isp er sio n of almost allcells Save
1975Hyde, GM;MILK YIELD AS A FACTOR IN DAIRY HERD MANAGEMENT BY EXCEPTION.ThesisTable 4 gives regression coefficients from the analyses over allcells, by parity, by age group, and by season of calving. Calculatedpeak times of lactation are also tabulated. Note that Save
1975McKEE, RH;THE CONTRIBUTION OF SINGLE-STRAND BREAKS AND ALKALI-LABILE BONDS TO THE LOSS OF INFECTIVITY OF GAMMA-IRRADIATED PHI-X174 REPLICATIVE FORM DNA IN E. COLI CELLS MUTANT IN VARIOUS REPAIR FUNCTIONSThesisTwenty-one radiation sensitive mutants have been examined for their capacity to support y-irradiated 0X174 RF-DNA. The survival of 0X174 RF-DNA was reduced in essentially all of Save
1975Fiske, RA;Klein, PA;Effect of immunosuppression on the genetic resistance of A2G mice to neurovirulent influenza virusInfection and immunity163800This putative replication defect, however, must be highly selective, ie, must occur in some but not allcells of the A2G mouse, since WSN can undergo complete cycles of replication in Save
1975Goldhammer, L;ATS-6 Solar Cell Flight Experiment After 325 Days in Synchronous OrbitIEEE Transactions on Aerospace and Electronic SystemsAllcells performed well through 325 days in orbit, except the FEP-covered cells, which appear to have increased their rate of degradation during the first eclipse season. Save
1975Schriber, S;Comparison of standing wave accelerators operating in the 2π/3 and π/2 modesIEEE Transactions on Nuclear ScienceThe singly periodic (allcells identical) 23 cell system used for experimental measurements of relative axial electric field amplitudes hadthe following mean parameters; Save
1974Baker, MA;Ramachandar, K;Taub, RN;Specificity of heteroantisera to human acute leukemia-associated antigensThe Journal of clinical investigation4140196Similarly, antisera to ALLcells absorbed with AML cells retained significant cytotoxicity only to ALL cells. Control antisera raised in nontolerant mice were cytotoxic to all cells tested. Save
1974Rutishauser, U;Yahara, I;Edelman, GM;Morphology, motility, and surface behavior of lymphocytes bound to nylon fibersProceedings of the National Academy of Sciences of the United States of America4545428Allcells were firmly attached to the fibers, did not detach even after incubation for 4 hr, and in no case was translocation of a cell along the fiber observed. A small number of cells nonspe Save
1974Stella, JP;Yankaskas, KD;Morgan, JH;Fox, MP;Pfau, CJ;Characteristics of the in vitro inhibition of arenavirus synthesis by bis-benzimidazolesAntimicrobial agents and chemotherapy4451346After an additional 24 h, allcells appeared to have normal morphologies, but the reticule counts from drug-treated cultures were 10% below those of the controls. Moreover, after Save
1974VAIL, AF;DROPOUT FROM PSYCHOTHERAPY AS RELATED TO PATIENT-THERAPIST DISCREPANCIES,THERAPIST CHARACTERISTICS, AND INTERACTION IN RACE AND SEXThesisThis could be co rre c te dfor by tran sfo rm in g th e num ber of rem ainers in allcells , as ifallcells had the same total of dropouts and rem ainers. I t was arbitrarily decided to treatall the Save
1974Maccallum, RC;A COMPARISON OF TWO INDIVIDUAL DIFFERENCES MODELS FOR MULTIDIMENSIONAL SCALING: CARROLL AND CHANG'S INDSCAL AND TUCKER'S THREE-MODE FACTOR ANALYSISThesisDue to high costs of generating replications of data sets to fit thisdesign, and then analyzing the data, it was not possible to represent allcells of Table 3 in the present study. Only the Save
1974Johnson, BS;IMITATION BY CHILDREN OF MODEL-PERFORMED BEHAVIOR UNDER A VARIETY OF STIMULUS CONDITIONS.ThesisA test of the equality of regression within allcells was performed, and the hypothesis of differentialwithin cell regression was not supported (F = .62; Save
1974VON BERGEN JR, CW;Individual differences variables and performance goals as determinants of level of performance.ThesisTo accomplish this a procedure was employed which essentially involved equating the n Ach scores for allcells ofthe self-esteem analysisandthe locusof Save
1974Burnstein, T;Jacobsen, LB;Zeman, W;Chen, TT;Persistent infection of BSC-1 cells by defective measles virus derived from subacute sclerosing panencephalitisInfection and immunity4215764Fluorescent measles antigen was present in allcells. Co-cultures of IP-3-Ca cells with normal BSC-1 cells developed massive syncytia and extensive destruction. The IP-3-Ca cells Save
1974Katsura, Y;Uesaka, I;Assessment of germ tube dispersion activity of serum from experimental candidiasis: a new procedure for serodiagnosisInfection and immunity4207514Allcells in the suspension were yeast form, with a few paired, but no germ tubes or pseudohyphae were found. Intraperitoneal (ip) infection was made by inoculating 0.5 ml of the Save
1974Heilmann, LJ;Physical studies of unfolded 30S ribosomal subunits of Escherichia coliThesisE ar ly work establishedthat th eseparticles were ap parentlyub iq u it ous in allcells and consisted of ap p ro x imately eq ualproportions of RNA and protein. E ar ly pre parations# called Save
1974Bendix, ME;A study of the adenyl cyclase activity in testis of maturing chinook salmon (Oncorhynchus tshawytscha)ThesisWithin each cyst allcells are at the same stage of spermatogenic development and are maturing at the same rate but within a testis the various cysts may be at different stages of Save
1973Warburton, D;Henderson, AS;Shapiro, LR;Hsu, LY;A stable human dicentric chromosome, t dic (12;14)(p13;p13) including an intercalary satellite region between centromeresAmerican journal of human genetics4716663Allcells from a peripheral blood culture had 45 chromosomes, with a missing D and C group chromosome and an abnormal large submetacentric chromosome with a prominent Save
1973BERNS, A;AYAD, S;Intergeneric Transformation in Bacillus subtilisBiochemical Society Transactionsallcells may be potentialrecipientsof fore ig n MA? as a consequence of th euniversalityof th egenet ic code - on thisplanetatleast*In many ca se s? up ta ke and " in tegration " offore ig n Save
1973Dadds, MJS;Detection and survival of Zymomonas in breweriesThesisMethods were developed for detecting zymomonads in beer and brewing materials to and the microbiological quality control of beer and to investigate the incidence and survival of Save
1972Eaton, DC;Potassium ion accumulation near a pace-making cell of AplysiaThe Journal of physiology5071402If it is assumed that the real capacitance of a small unit area of membrane is the same for allcells in Aplysia, then capacitances larger than expected represent an underestimation of the Save
1972Munthe, E;Natvig, JB;Immunglobulin classes, subclasses and complexes of IgG rheumatoid factor in rheumatoid plasma cellsClinical and experimental immunology4563335Allcells binding RB200 conjugate fixed also the FITC conjugate in the second step because of binding either to the plasma cell rheumatoid factor Ig or to the added RB200-labelled Save
1972Sander, G;Jacobs, JP;Observations on interactions between fluorescein-iso-thiocyanate conjugates and cells in vitroJournal of clinical pathology4114699At each passage allcells were treated in an identical manner. The methods employed were as described in a previous communication by Sander (1969) and Sander et al (1970). The Save
1972Penn, RF;An experimental study involving the use of contract evaluation in a chem study and in a traditional high school chemistry courseThesisTEST OF EQUALITY OFREGRESSION IN ALLCELLS Save
1972Robertson, A;Cohen, MH;Control of developing fieldsAnnual review of biophysics and bioengineering4405416Wolpert's definition, "a field is that region in which allcells are having their positional information specified with respect to the same set of points," is the most useful one for our purposes, Save
1971Blankley, WF;Auxotrophic and heterotrophic growth and calcification in coccolithophoridsThesisAllcells retained a complete coccolith cover throughout the experiment(see also Fig. IV.?). Allcells retained a complete coccolith cover throughout the experiment. Save
1971BIEGEN, DA;The effects of irrelevant and immaterial data on problem difficultyThesisId ea lly , th is 'coefficient ' would be app lica b le w ith in allcells . Save
1971Jacobs, NJ;Jacobs, JM;Brent, P;Characterization of the late steps of microbial heme synthesis: conversion of coproporphyrinogen to protoporphyrinJournal of bacteriology4935319similar in allcells, this reaction should be demonstrable in all heme-containing bacteria. In addition, we also partially characterized the enzyme system in E. coli, which converts copropor Save
1970Brooks, JE;Disuse atrophy of muscle. Intracellular electromyographyArchives of neurology5409598The amount of depolarization necessary to induce an action potential was measured in allcells stimulated. The critical depolari¬ zation (CD) in normal fibers ranged from 6 mv to 36 mv Save
1970Jayaraman, S;Radiation Damage and Repair After Wholebody Or Partial-body Irradiations of Tenebrio Molitor LarvaeThesisBecause during the larval metamorphosis, not allcells divide simultaneously and because their growth is discon¬tinuous, it is conceivable that exposures that do not cause thedeath of Save
1970Mehall, AG;Capacity of anterior pituitary grafts to correct modified growth and development of selected long bones of hypophysectomized chick embryosThesis3 no morphological differentiation in limbbud; shapes of bones pre-determlned; allcells of bud equivalent and may formcartilage or muscle up to stage 22 Save
1970Jones, JM;Schwab, JH;Effects of streptococcal cell wall fragments on phagocytosis and tissue culture cellsInfection and immunity16557722Allcells containing one or more bacteria were counted. No correction was attempted for bacteriawhich may have only settled on top of phagocytic cells and were not taken up, since it Save
1969McClure, HM;Belden, KH;Pieper, WA;Jacobson, CB;Autosomal trisomy in a chimpanzee: resemblance to Down's syndromeScience (New York, N.Y.)4240970Cells containing fewer than 49 chromosomes probably resulted from excess spreading during hypotonic treatment, since allcells showed apparent trisomy of a small acrocentric. This Save
1969Whitman, MA;Discrimination Learning as a Function of MA, IQ, and InstitutionalizationThesisnumber of correct responses for the groupswhich were involved in computing these 5 tests are listed in Table 4(see Appendix 2 for average performance and average latency of allcells) Save
1969Jacoby, RO;Studies in canine immunobiology with special reference to mechanisms of lymphoid cell depletion in viral diseaseThesisIn virus-induced tumors, allcells transformed by a given v irus have a common TSTA regardless of the morphology of the tumor or its host of or ig in , but the TSTAs of tumors induced by Save
1969Gordon, MW;Neuronal plasticity and memoryThe American journal of orthopsychiatry4896079Some have been mentioned which are associated with the synaptic mem brane but allcells contain enzymes that direct incoming and outgoing metabolic traffic. Recently, an Save
1969Grady, CP;Gaudy, AF;Control Mechanisms Operative in a Natural Microbial Population Selected for Its Ability to Degrade L-Lysine. II. Effects of Fructose and Ribose in Batch SystemsApplied microbiology16349866Hence, not allcells of the population were capable of utilizing ribose. Since this had not been encountered with the carbon sources previously used, further selection was not carried out. Save
1969Baron, DN;Down with plasma. Intracellular chemical pathology studied by analysis of cells of solid tissues, erythrocytes, and leukocytesProceedings of the Royal Society of Medicine4900112For allcells, because of the technical problems involved, it had been usual to consider the cells with their accompanying extracellular fluid as a single tissue. This assumed, not neces Save
1968Bernheimer, AW;Cytolytic toxins of bacterial origin. The nature and properties of cytolytic proteins are discussed with emphasis on staphylococcal alpha-toxinScience (New York, N.Y.)4295145All cells that are sensitive to streptolysin 0 contain cholesterol as a constituent of their cell membranes, and allcells so far examined that lack membrane cho- Save
1968Bolognesi, DP;Langlois, AJ;Sverak, L;Bonar, RA;Beard, JW;In vitro chick embryo cell response to strain MC29 avian leukosis virusJournal of virology4301506has disclosed cultural changes of two principal kinds: (i) behavior of the virus-exposed cells paralleling that of the control cultures related presumably to environmental effects on allcells Save
1968Fielding, S;Auditory Adaptation: Evidence of DuplexityThesisThis 2 0 - ce ll experiment was rep lic a te d fiv e tim es; allcells in one replicat io n were completed b efo re the nex treplicat io n began. Save
1968Walters, RA;Petersen, DF;Radiosensitivity of Mammalian Cells: I. Timing and Dose-Dependence of Radiation-Induced Division DelayBiophysical JournalIt appears that not only do allcells recover the ability to traverse thelife cycle and divide after irradiation with relatively small doses but do so for significant periods of time without Save
1968Bloodworth, JM;Wers, KL;The ultrastructure of the normal dog adrenalJournal of anatomy5656137Adrenal capsule composed of elongated connective tissue cells (CT), basement membrane (BM) which surrounds most but not allcells (arrow), and collagen fibrils (C). x 6500. Fig. Save
1967Epel, D;Protein synthesis in sea urchin eggs: a "late" response to fertilizationProceedings of the National Academy of Sciences of the United States of America5232369Furthermore, autoradiographic data show that this protein synthesis occurs in allcells of the population, so that the observed incorporation cannot be attributed to the presence of Save
1967Carstensen, EL;Smearing, RW;Dielectric properties of osmium-fixed erythrocytesIEEE transactions on bio-medical engineering6078983Above 50 iIHz, the effective homiiogeneous conductivity for allcells described in Figs. and 3 is equal to the internal conductivity. The reactance of the imiembrane is negligible above Save
1967Panteleakis, PN;STUDIES OF NUCLEOLAR MORPHOLOGY AND CYTOCHEMISTRY AFTER ALTERATION OF RNA, AND PROTEIN SYNTHESISThesisS ince allcells tr ea te d in thism anner g iv es im ila r results , con tr o ls w ill not be repor te The firsteffectof actin omy cin D on allcells , w ith th eexcep tio nof HEp. No. 2 become d is Save
1967Hoff, BJ;The Cytology and Fertility of Bermudagrass, Cynodon Dactylon (L.) Pers., in ArizonaThesisHowever this was not the case in allcells and in cells from plant 41, with 3 accessory chromo¬somes, sometimes none, one, two or all three accessorychromosomes divided precociously Save
1967Tamura, A;Matsumoto, A;Higashi, N;Purification and chemical composition of reticulate bodies of the meningopneumonitis organismsJournal of bacteriology6025310When- almost allcells were infected, they were theni harvested, andthe cells were renioved by centrifugation at 300 X g for 10 nmimi. The organisimis in the superinatanit fluid were Save
1967Neill, C;Guest, WC;Effects of Hydroxyurea on Cultured Somatic Cells of the Chinese Hamster, Cricetulus GriseusThesisAllcells within the grid and the cells in some stage of cell division were scored. Ten randomly chosen fields were counted on each replicate. A mitotic index, calculated according to the Save
1966Gartland, WJ;Sueoka, N;Two interconvertible forms of tryptophanyl sRNA in E. coliProceedings of the National Academy of Sciences of the United States of America5327074Induction of cellular DNA synthesis by polyoma virus had so far been observed under conditions where not allcells were productively infected.In contrast with these findings, Sheinin Save
1966Vogt, M;Dulbecco, R;Smith, B;Induction of cellular DNA synthesis by polyoma virus. 3. Induction in productively infected cellsProceedings of the National Academy of Sciences of the United States of America4286763Induction of cellular DNA synthesis by polyoma virus had so far been observed under conditions where not allcells were productively infected.In contrast with these findings, Sheinin Save
1965Marcus, PI;Carver, DH;Hemadsorption-negative plaque test: new assay for rubella virus revealing a unique interferenceScience (New York, N.Y.)4283964In contrast, Echo-ll, polio-I, vaccinia, and influenza B viruses produce gross cytopathic changes in essentially allcells of the monolayer within 24 hours. A noncytopathic, hemadsorbing Save
1965Barker, CL;Maternal childrearing history and subsequent sensitivity to shame in college malesThesisLevel of illu m in atio n scores and level of sound scores co rre la te dpos itiv e ly in allcells , but only one of the eight £ 's was s ig n ific an t. O ther than this , th ere appeared to be no sys Save
1965MAC DONALD, C;AUTORADIOGRAPHY OF THYMIDINE-TRITIUM AND LYSINE-TRITIUM INCORPORATED INTO HUMAN CHROMOSOMESThesisThe process of duplication of cellular organellesimplied by the wcell theory" and the notion that allcells are derived from other cells, stands as a funda¬ mental concept of biology. The Save
1964ZIEVE, PD;GAMBLE, JL;JACKSON, DP;EFFECTS OF THROMBIN ON THE POTASSIUM AND ATP CONTENT OF PLATELETSThe Journal of clinical investigation14223918platelets produced by thrombin apparently was not due to a general effect on allcells, since thrombin did not induce a loss of potassium from red blood cells. The mechanism of action of Save
1964HINZE, HC;WALKER, DL;RESPONSE OF CULTURED RABBIT CELLS TO INFECTION WITH THE SHOPE FIBROMA VIRUS. I. PROLIFERATION AND MORPHOLOGICAL ALTERATION OF THE INFECTED CELLSJournal of bacteriology14219036Typical cytoplasmic inclu-sions appear at this time in virtually allcells of the infected culture. No other changes in cell morphology are apparent at this time. Eosin and hematoxylin stain. Save
1962Weiss, JE;THE EFFECT OF PROFESSIONAL TRAINING, AMOUNT OF INFORMATION, AND CORRECTNESS OF INFORMATION ON ACCURACY OF PREDICTION.Thesisd id not d is tr ib u te evenly over allcells , it was n ecessary Save
1960Kallenbach, E;DNA synthesis in the cells of the thymic cortex of the rat as shown in radioautographs after administration of Thymidine-H^ 3.ThesisSainte-Marie and Leblond (1958a) COQ~ted allcells seen in section that is also calottes of cells which were lying with the greater part of their volume outside the section. This procedure Save
1959Higgins, TW;New Polyacrylamide Gel Separators for the Le Clanche CellThesisP r io r to d is charge the open circuit vo ltage of allcells was measured to avoid te st in g an obviously de fec tiv ecell .The cel ls were prepared ford ischarge by so ld e rin g sh o rt lead Save
1958MANDELSTAM, J;Turnover of protein in growing and non-growing populations of Escherichia coliThe Biochemical journal13535591From the data available it is not possible to tell whether turnover occurs in allcells of a nongrowing population or whether all proteins are involved. A number of observations, however, Save
1957Somerville, RL;In vitro studies of purine nucleotide biosynthesis in rat intestinal mucosaThesisthe various forms of living matter pass on to their progeny their various specifically organized patterns of growth and metabolism and are prominent constituents of the nuclei of allcells (2 Save
1955ISING, U;Chromosome studies in Ehrlich mouse ascites cancer after heterologous transplantation through hamstersBritish journal of cancer133042184, which also suggests a narrowing of the scatter of the chromo-some numbers, the modal class containing as many as 39 per cent of allcells, as against only 25 per cent in the mouse Save
1955Angelotti, R;Studies on the physiological activities of terramycin-resistant and terramycin-susceptible cultures of cheese-ripening strains of Streptococcus lactisThesisUpon exposure to phage, allcells would presumably be sensitive and the number of resist¬ant bacteria obtained from a series of similar samples shouldshow only the fluctuation due to Save
1951Deschner, EE;The Relationship Between Ribonucleic Acid and the Gram Staining ReactionThesis, the morphology of the cells was not changed but some of the cells were completely Gram negative in character, yet no signs of any stages of digestion were present, that is, allcells Save
1951Williams, JLD;Enchondroma of the Nasal Cavities, and its Relationship to Other Cartilaginous Tumours in This RegionThesisnantof neop lasm s, us in g th eparasiticfoe tu ses , tera to ma ta and mixed tum ours as s te pp in gs to nes * Thus, al th ough th ereisanaturalfacility forlimitlessg ro w th in near ly allcells Save
1950Smith, GM;Regnery, DC;Inheritance of Sexuality in Chlamydomonas ReinhardiProceedings of the National Academy of Sciences of the United States of America16578332For all species investigated he finds exceptional cases in which allcells produced by a germinating zygote are homothallic (monoecious) instead of all being heterothallic. For zygotes Save
1950Jehle, H;Specificity of Interaction Between Identical MoleculesProceedings of the National Academy of Sciences of the United States of America16578331For all species investigated he finds exceptional cases in which allcells produced by a germinating zygote are homothallic (monoecious) instead of all being heterothallic. For zygotes Save
1941Loofbourow, JR;Dwyer, CM;Cronin, AG;Proliferation-promoting intercellular hormones: Evidence for their production by living cells as a response to injuryThe Biochemical journal16747427Both were then irradiated in quartz test tubes with full ultraviolet until allcells in the unboiled suspension were killed (methylene.blue test). The irradiation time in the experiment Save
1934Tryon, R;A correlation form with absolute checks.Journal of Experimental PsychologyIn each row of quadrant 1, Multiply the frequency in each cell tines the nuwber in the upper corner of the cell, ant) SUB all these for allcells in that row, placing the total In the proper Save
1929McDonald, E;CELLULAR DIVISION IN RELATION TO CANCERScience (New York, N.Y.)17733743The cell reacts as a unit and there are certain phenomena which are common to allcells. There are regularities in the complexity of cell phenomena which make it possible to study cell Save
1926Orahovats, D;The spleen and the resistance of red cellsThe Journal of physiology16993804First a group which is most resistant and which forms about 10 pc of allcells, then a group to which the greatest part of the red cells belong, about 80 pc, with a middle resistance, and a Save
1920Mott, FW;Studies in the Pathology of Dementia PraecoxProceedings of the Royal Society of Medicine19981338This applies to allcells including those of the olivary body, although the pigment in this kind of cell is finer. The large cells show the lipoid granules better. Unstained Specimen of Medulla Save
1911Paine, A;Nicholson, GW;SURGICAL INTERFERENCE IN CANCER: (AN EXPERIMENTAL STUDY.)British medical journal20765743As allcells have an age limit, at the end of which they die and towards the close of whichthey become old and their activities diminish, itfollows that the isolated nodules, which consist Save
1905MARCY, H;THE CURE OF HERNIA.Journal of the American Medical Associationfor 30 minutes and not allcells being killed during one hour at this temperature. This may explain the fact that the virus is not always killed by cooking the meat. The organism probably Save
1874Mahomed, FA;The Relation between Arterial Tension and AlbuminuriaBritish medical journal20747530These are cast off in great numbers, and, blocking the tubules, form the large white kidney; here they undergo the change common to allcells after their death-namely, molecular Save
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2023Aashna Karbhari ,Sherly Mosessian ,Kamaxi H. Trivedi,Frank Valla Jr,Mark Jacobson,Mark J. Truty,Nandakumar G. Patnam,Diane M. Simeone,Elcin Zan,Tracy Brennan,Hongli Chen,Phillip H. Kuo,Ken Herrmann,Ajit H. GoenkaGallium-68-labeled fibroblast activation protein inhibitor-46 PET in patients with resectable or borderline resectable pancreatic ductal adenocarcinoma: A phase 2, multicenter, single arm, open label non-randomized study protocolPLOS OneFull Paper
2023Cheong, JG;Ravishankar, A;Sharma, S;Parkhurst, CN;Grassmann, SA;Wingert, CK;Laurent, P;Ma, S;Paddock, L;Miranda, IC;Karakaslar, EO;Nehar-Belaid, D;Thibodeau, A;Bale, MJ;Kartha, VK;Yee, JK;Mays, MY;Jiang, C;Daman, AW;Martinez de Paz, A;Ahimovic, D;Ramos, V;Lercher, A;Nielsen, E;Alvarez-Mulett, S;Zheng, L;Earl, A;Yallowitz, A;Robbins, L;LaFond, E;Weidman, KL;Racine-Brzostek, S;Yang, HS;Price, DR;Leyre, L;Rendeiro, AF;Ravichandran, H;Kim, J;Borczuk, AC;Rice, CM;Jones, RB;Schenck, EJ;Kaner, RJ;Chadburn, A;Zhao, Z;Pascual, V;Elemento, O;Schwartz, RE;Buenrostro, JD;Niec, RE;Barrat, FJ;Lief, L;Sun, JC;Ucar, D;Josefowicz, SZ;Epigenetic memory of coronavirus infection in innate immune cells and their progenitorsCell37597510Paired PBMC and BMMC AllCells N/A Whole blood from study participants Weill-Cornell Medicine/New York-Presbyterian Hospital N/AFull Paper
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2023Fiumara, M;Ferrari, S;Omer-Javed, A;Beretta, S;Albano, L;Canarutto, D;Varesi, A;Gaddoni, C;Brombin, C;Cugnata, F;Zonari, E;Naldini, MM;Barcella, M;Gentner, B;Merelli, I;Naldini, L;Genotoxic effects of base and prime editing in human hematopoietic stem cellsNature biotechnology37679541Granulocyte colony-stimulating factor (G-CSF) or G-CSF + plerixafor mPB CD34+ HSPCs were purified in-house with the CliniMACS CD34 Reagent system (Miltenyi Biotec) from Mobilized Leukopak (AllCells) according to the TIGET-HPCT protocol approved by the OSR Ethical Committee and following the manufacturer’s instructions. HSPCs were seedeFull Paper
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2023Cascone, T;Kar, G;Spicer, JD;Garcia-Campelo, R;Weder, W;Daniel, DB;Spigel, DR;Hussein, M;Mazieres, J;Oliveira, J;Yau, EH;Spira, AI;Anagnostou, V;Mager, R;Hamid, O;Cheng, LY;Zheng, Y;Blando, J;Tan, TH;Surace, M;Rodriguez-Canales, J;Gopalakrishnan, V;Sellman, BR;Grenga, I;Soo-Hoo, Y;Kumar, R;McGrath, L;Forde, PM;Neoadjuvant Durvalumab Alone or Combined with Novel Immuno-Oncology Agents in Resectable Lung Cancer: The Phase 2 NeoCOAST Platform TrialCancer discovery37707791Messenger RNA was extracted from pre-treatment and on-treatment FFPE tumor tissue, when available, using the HudsonAlpha Discovery FFPE Tissue Extraction Method SaveFull Paper
2023Saura, C;Ortiz, C;Matito, J;Arenas, EJ;Suñol, A;Martín, Á;Córdoba, O;Martínez-Sabadell, A;García-Ruiz, I;Miranda, I;Morales-Comas, C;Carrasco, E;Viaplana, C;Peg, V;Nuciforo, P;Bayó-Puxan, N;Gonzalez-Medina, A;Miquel, JM;Gómez-Rey, M;Villacampa, G;Arévalo, S;Espinosa-Bravo, M;Balmaña, J;Dienstmann, R;Arribas, J;Tabernero, J;Vivancos, A;Sansó, M;Early-Stage Breast Cancer Detection in Breast MilkCancer discovery37704212f the study. V. Peg reports personal fees from Roche, AstraZeneca, Sysmex, and Merck Sharp & Dohme outside the submitted work. P. Nuciforo reports personal fees from Bayer, MSD Oncology, Novartis, and Targos Molecular Pathology GmbH outside the submitted work. M. Gómez-Rey reports nonfinancial support from the Cellex Foundation during the conduct of the study. G. Villacampa reports persoFull Paper
2023Reggiardo, RE;Maroli, SV;Peddu, V;Davidson, AE;Hill, A;LaMontagne, E;Aaraj, YA;Jain, M;Chan, SY;Kim, DH;Profiling of repetitive RNA sequences in the blood plasma of patients with cancerNature biomedical engineering37652985ExoRNeasy kit (Qiagen) was used to isolate cell-free RNA from blood plasma of de-identified healthy controls (blood collected in K2EDTA tubes; Discovery Life Sciences) SaveFull Paper
2023Li, Y;Xue, M;Deng, X;Dong, L;Nguyen, LXT;Ren, L;Han, L;Li, C;Xue, J;Zhao, Z;Li, W;Qing, Y;Shen, C;Tan, B;Chen, Z;Leung, K;Wang, K;Swaminathan, S;Li, L;Wunderlich, M;Mulloy, JC;Li, X;Chen, H;Zhang, B;Horne, D;Rosen, ST;Marcucci, G;Xu, M;Li, Z;Wei, M;Tian, J;Shen, B;Su, R;Chen, J;TET2-mediated mRNA demethylation regulates leukemia stem cell homing and self-renewalCell stem cell37541212TET2 is recurrently mutated in acute myeloid leukemia (AML) and its deficiency promotes leukemogenesis (driven by aggressive oncogenic mutations) and enhances Save;Recombinant Mouse IFN-b R&D SYSTEMS Cat# 8234-MB-010 ColonyGEL-Mouse Base Medium Reachbio Cat# 1201 FastDigest NotI Thermo Fisher Scientific Cat# FD0595Full Paper
2023Juric, V;Mayes, E;Binnewies, M;Lee, T;Canaday, P;Pollack, JL;Rudolph, J;Du, X;Liu, VM;Dash, S;Palmer, R;Jahchan, NS;Ramoth, ÅJ;Lacayo, S;Mankikar, S;Norng, M;Brassell, C;Pal, A;Chan, C;Lu, E;Sriram, V;Streuli, M;Krummel, MF;Baker, KP;Liang, L;TREM1 activation of myeloid cells promotes antitumor immunityScience translational medicine37647386Dissociated human tumor cells (Discovery Life Sciences) were stained with Zombie NIR viability dye, FcR-blocked with Human TrueStain FcX (BioLegend), and stained SaveFull Paper
2023Hong, D;Jo, EJ;Bang, D;Jung, C;Lee, YE;Noh, YS;Shin, MG;Kim, MG;Plasmonic Approach to Fluorescence Enhancement of Mesoporous Silica-Coated Gold Nanorods for Highly Sensitive Influenza A Virus Detection Using Lateral Flow ImmunosensorACS nano37595106IAV and IBV patient samples for discovery cohort analysis were purchased from Discovery Life Sciences (Los Osos, CA). IAV patient samples for validation cohort analysis SaveFull Paper
2023Worboys, JD;Vowell, KN;Hare, RK;Ambrose, AR;Bertuzzi, M;Conner, MA;Patel, FP;Zammit, WH;Gali-Moya, J;Hazime, KS;Jones, KL;Rey, C;Jonjic, S;Rovis, TL;Tannahill, GM;Cruz De Matos, GDS;Waight, JD;Davis, DM;TIGIT can inhibit T cell activation via ligation-induced nanoclusters, independent of CD226 co-stimulationNature communications37596248Tumour samples and associated reference peripheral blood samples were acquired from the commercial vendor Discovery Life Sciences under Task Order DISLS06 as Save;f Manchester, UK) as approved by the West Midlands - Black Country Research Ethics Committee. Tumour samples and associated reference peripheral blood samples were acquired from the commercial vendor Discovery Life Sciences under Task Order DISLS06 as approved by GSK HBSM Due Diligence. Cell lines The Jurkat cell line was obtained from the ATCC (Clone E6-1; ATCC TIB-152 ; RRID:CVCL_0367) andFull Paper
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2023Bekerman, E;Yant, SR;VanderVeen, L;Hansen, D;Lu, B;Rowe, W;Wang, K;Callebaut, C;Long-acting lenacapavir acts as an effective preexposure prophylaxis in a rectal SHIV challenge macaque modelThe Journal of clinical investigation37384413Human PBMCs were collected from healthy volunteers after informed consent, and their use was approved by an institutional review board at AllCells (Alameda, California SaveFull Paper
2023Lin, W;Zhang, Y;Yang, Y;Lin, B;Zhu, M;Xu, J;Chen, Y;Wu, W;Chen, B;Chen, X;Liu, J;Wang, H;Teng, F;Yu, X;Wang, H;Lu, J;Zhou, Q;Teng, L;Anti-PD-1/Her2 Bispecific Antibody IBI315 Enhances the Treatment Effect of Her2-Positive Gastric Cancer through Gasdermin B-Cleavage Induced PyroptosisAdvanced science (Weinheim, Baden-Wurttemberg, Germany)37587833lone) supplemented with 10% FBS. Human PBMCs (P121080605C and LP220727010) were obtained from ALLCELLS (Shanghai, China) and cultured in RPMI medium with 10% heat-inactivated FBSFull Paper
2023Marone, R;Landmann, E;Devaux, A;Lepore, R;Seyres, D;Zuin, J;Burgold, T;Engdahl, C;Capoferri, G;Dell'Aglio, A;Larrue, C;Simonetta, F;Rositzka, J;Rhiel, M;Andrieux, G;Gallagher, DN;Schröder, MS;Wiederkehr, A;Sinopoli, A;Do Sacramento, V;Haydn, A;Garcia-Prat, L;Divsalar, C;Camus, A;Xu, L;Bordoli, L;Schwede, T;Porteus, M;Tamburini, J;Corn, JE;Cathomen, T;Cornu, TI;Urlinger, S;Jeker, LT;Epitope-engineered human hematopoietic stem cells are shielded from CD123-targeted immunotherapyThe Journal of experimental medicine37773046and the plate was incubated at 37°C. CD123 and CCR5 gene editing with CRISPR/Cas9 and rAAV6 virus CD34+ HSPC culture condition Plerixafor-mobilized peripheral blood CD34+ HSPCs were purchased from AllCells. The cells were thawed as per the manufacturer’s instructions and cultured at 37°C, 5% CO2, and 5% O2. Cell culture medium was GMP stem cell growth medium (SCGM) medium from CellGenix suppFull Paper
2023Chen, C;Shen, M;Wan, X;Sheng, L;He, Y;Xu, M;Yuan, M;Ji, Z;Zhang, J;Activated T cell-derived exosomes for targeted delivery of AXL-siRNA loaded paclitaxel-poly-L-lysine prodrug to overcome drug resistance in triple-negative breast cancerChemical Engineering JournalAcademy of Sciences (Shanghai, China). Human PBMCs (hPBMCs) were purchased from AllCells Technologies (Alameda, USA). All experiments conducted with human samples were approvFull Paper
2023Reyes-Castro, RA;Chen, SY;Seemann, J;Kundu, ST;Gibbons, DL;Arur, S;Phosphorylated nuclear DICER1 promotes open chromatin state and lineage plasticity of AT2 tumor cells in lung adenocarcinomasScience advances37494452Tumor microarrays (TMAs) with de-identified patient information were obtained from Discovery Life Sciences (Powell, OH) and TriStar Technology Group LLC (Washington Save;oth genders were used. No power analysis was used to determine the sample size. Human LUAD tumor tissue microarrays Tumor microarrays (TMAs) with de-identified patient information were obtained from Discovery Life Sciences (Powell, OH) and TriStar Technology Group LLC (Washington, DC). Eighty-eight LUAD samples from untreated patients were used. No patient or human subjects were recruited for thiFull Paper
2023Fischer, M;Gerrick, K;Belmontes, B;Slemmons, K;Freyman, Y;Jain, J;Federowicz, S;Bishof, I;Rao, A;Fleury, M;Mounir, Z;Lackner, M;Hughes, P;White, M;Neilan, C;Abstract 1644: Dual inhibition of MAT2A and PRMT5 delivers synergistic anti-tumor responses in preclinical models of MTAP-deleted cancerCancer ResearchPharmaron, Charles River, Wuxi Apptec, Champions Oncology, Xentech, and Crown Biosciences for performing in vivo studies 2. HudsonAlpha Discovery for RNA sequencing 3. Fios Genomics and Directed Bio for gene expression and splicing analysis 4. Mosaic Laboratories for SDMA IHCFull Paper
2023Gerrick, K;Laraio, J;Annen, K;Tran, H;Fischer, M;Freyman, Y;Sharma, G;Bishof, I;Federowicz, S;Pankajakshan, D;Neilan, C;Mangatt, B;Mazurek, A;Lackner, M;White, M;Mounir, Z;Abstract 1637: MAT2A inhibition in MTAP-/- tumors confers mechanistic vulnerabilities to multiple clinically actionable synthetic lethal drug combinationsCancer Researchable contributions from Dr. Christian Frey, Dr. Jeanne Quirit, Dr. Neil Bhola, Atieh Givmanesh, and John Faulhaber. We are grateful to our CRO partners Horizon Discovery, Eurofins, DepMap Consortium, and HudsonAlpha DiscoveryFull Paper
2023Kumar, A;Estes Bright, LM;Garren, MRS;Manuel, J;Shome, A;Handa, H;Chemical Modification of Tiopronin for Dual Management of Cystinuria and Associated Bacterial InfectionsACS applied materials & interfaces37671841The urine sample was obtained from Discovery Life Sciences (Huntsville, AL) and work was performed according to the Use of Laboratory Sample Guidelines by the Save;b holders, sputter-coated with gold-palladium, and observed with an FEI Teneo Scanning Electron Microscope (Thermo Fisher, Waltham, MA). Urine Sample Preparation The urine sample was obtained from Discovery Life Sciences (Huntsville, AL) and work was performed according to the Use of Laboratory Sample Guidelines by the University of Georgia to ensure appropriate handling and disposal of clinicaFull Paper
2023Kannan, K;Kumar, K;Dhivagar, R;Sathyanarayanan, S;Parameshwaran, R;Singh, P;An experimental study on heat power stored by thermal dissipation in electronic components using thermosyphon integrated hybrid nanocomposite phase change materialJournal of Energy StorageThe melting ranges of the three PCMs used were 42 °C, 55 °C and 58 °C for PureTemp, allcells PCC, and pure paraffin wax, respectively. The operating time of electronic equipment is reportedly increased as a result of PCM's ability to stabilise the device temperatFull Paper
2023Macaulay, VM;Lord, S;Hussain, S;Maroto, JP;Jones, RH;Climent, MÁ;Cook, N;Lin, CC;Wang, SS;Bianchini, D;Bailey, M;Schlieker, L;Bogenrieder, T;de Bono, J;A Phase Ib/II study of IGF-neutralising antibody xentuzumab with enzalutamide in metastatic castration-resistant prostate cancerBritish journal of cancer37537253assessed by immunohistochemistry (IHC) in archival formalin-fixed, paraffin-embedded (FFPE) tumour samples and was undertaken centrally by Discovery Life Sciences ( Save;lation in cells expressing human IGF-1R. PTEN expression was assessed by immunohistochemistry (IHC) in archival formalin-fixed, paraffin-embedded (FFPE) tumour samples and was undertaken centrally by Discovery Life Sciences (Kassel, Germany) using the antibody clone 138G6 (cat #9559, Cell Signaling Technology) and the fully automated Ventana Benchmark Ultra platform following the Ultra View DAB prFull Paper
2023Sheng, XR;Gao, X;Schiffman, C;Jiang, J;Ramalingam, TR;Lin, CJF;Khanna, D;Neighbors, M;Biomarkers of fibrosis, inflammation, and extracellular matrix in the phase 3 trial of tocilizumab in systemic sclerosisClinical immunology (Orlando, Fla.)37479123A separate set of HC serum samples (HC2s; collected by Discovery Life Sciences) was Discovery Life Sciences and BioIVT provided ethically collected healthy donor Save;ained from the Genentech Samples for Science program (South San Francisco, CA), Discovery Life Sciences (Huntsville, AL), and BioIVT (Hicksville, NY) was processed and stored according to standard sampling procedures, and assayed for CCL18, YKL-40, CXCL13, COMP,Full Paper
2023Pujals, M;Mayans, C;Bellio, C;Méndez, O;Greco, E;Fasani, R;Alemany-Chavarria, M;Zamora, E;Padilla, L;Mitjans, F;Nuciforo, P;Canals, F;Nonell, L;Abad, M;Saura, C;Tabernero, J;Villanueva, J;RAGE/SNAIL1 signaling drives epithelial-mesenchymal plasticity in metastatic triple-negative breast cancerOncogene37468678Epithelial/Mesenchymal (E/M) plasticity plays a fundamental role both in embryogenesis and during tumorigenesis. The receptor for advanced glycation end products ( SaveFull Paper
2023Birker, K;Ge, S;Kirkland, NJ;Theis, JL;Marchant, J;Fogarty, ZC;Missinato, MA;Kalvakuri, S;Grossfeld, P;Engler, AJ;Ocorr, K;Nelson, TJ;Colas, AR;Olson, TM;Vogler, G;Bodmer, R;Mitochondrial MICOS complex genes, implicated in hypoplastic left heart syndrome, maintain cardiac contractility and actomyosin integrityeLife37404133WGS was performed on samples from 183 individuals with HLHS and 496 family members by the Mayo Clinic Medical Genome Facility or Discovery Life Sciences. SNVs and INDELs that passed quality control were subject to further filtering based upon rarity (MAF 0.01) and predicted consequence. Details about the sequencing and subsequent variant filteriFull Paper
2023Khan, DMIO;Karmaus, PWF;Bach, A;Crawford, RB;Kaminski, NE;An in vitro model of human hematopoiesis identifies a regulatory role for the aryl hydrocarbon receptorBlood advances37477592In vitro models to study simultaneous development of different human immune cells and hematopoietic lineages are lacking. We identified and characterized, using single- Save;Human CD34+ 91 HSPCs isolated from umbilical cord blood from mixed donors were purchased in cryopreserved state from AllCells LLC (CA, USA). ApproxiFull Paper
2023Klein, S;Schulte, A;Arolt, C;Tolkach, Y;Reinhardt, HC;Buettner, R;Quaas, A;Intratumoral Abundance of M2-Macrophages is Associated With Unfavorable Prognosis and Markers of T-Cell Exhaustion in Small Cell Lung Cancer PatientsModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc37423586RB is co-founder and scientific advisor for Targos Molecular Pathology Inc and member of the Directory Board Gnothis Inc. HCR received consulting and lecture fees from SaveFull Paper
2023Jia, Z;Li, Z;Liu, C;CRISPR-powered Biosensing Platform for Quantitative Detection of Alpha-fetoprotein by a Personal Glucose MeterSensors and actuators. B, Chemical37303825Serum samples from patients with liver cancer were obtained from Discovery Life Sciences. Human AFP ELISA kits were purchased from RayBiotech, Inc. PGMs (Accu- Save;ns, Inc. Serum samples from healthy individuals were obtained from Innovative Research, Inc. Serum samples from patients with liver cancer were obtained from Discovery Life Sciences. Human AFP ELISA kits were purchased from RayBiotech, Inc. PGMs (Accu-Chek Aviva Plus) and test strips were purchased from Roche Diagnostics Co., Ltd. All chemicaFull Paper
2023Leatham, B;McNall, K;Subramanian, HKK;Jacky, L;Alvarado, J;Yurk, D;Wang, M;Green, DC;Tsongalis, GJ;Rajagopal, A;Schwartz, JJ;A rapid, multiplex digital PCR assay to detect gene variants and fusions in non-small cell lung cancerMolecular oncology37714814A second set of de-identified, remnant FFPE samples greater than 15 years old were sourced from Discovery Life Sciences (includes samples through acquisitions of Save;- Digital PCR (dPCR) is emerging as an ideal platform for the detection and tracking of genomic variants in cancer due to its high sensitivity and simple workflow. The SaveFull Paper
2023Sullivan, AT;Rao, V;Rockwood, T;Gandhi, J;Gruzka, S;O'Connor, L;Wang, B;Ragan, KB;Zhang, DY;Khodakov, D;Rapid, tunable, and multiplexed detection of RNA using convective array PCRCommunications biology37741867VTM samples testing negative for presence of SARS-CoV-2 were purchased from Discovery Life Sciences' sample repository. Sample lysis and nucleic acid purification SaveFull Paper
2023Salek, M;Li, N;Chou, HP;Saini, K;Jovic, A;Jacobs, KB;Johnson, C;Lu, V;Lee, EJ;Chang, C;Nguyen, P;Mei, J;Pant, KP;Wong-Thai, AY;Smith, QF;Huang, S;Chow, R;Cruz, J;Walker, J;Chan, B;Musci, TJ;Ashley, EA;Masaeli, MM;COSMOS: a platform for real-time morphology-based, label-free cell sorting using deep learningCommunications biology37740030lls and cancer cells before and after CD45 depletion. The remaining sample was processed, and A549 cells were sorted on COSMOS. Dissociated tumor cells (DTCs) from NSCLC patients were purchased from Discovery Life Sciences (DLS; Los Osos, CA, USA). Cancer type, stage information, and cell type composition reports from flow cytometry were provided by the vendor. To account for possible cell type cFull Paper
2023Gail, D;Suzart, V;Du, W;Kaur Sandhu, A;Jarvela, J;Nantongo, M;Mwebaza, I;Panigrahi, S;Freeman, M;Canaday, D;Boom, W;Silver, R;Carpenter, S;Mycobacterium tuberculosis impairs human memory CD4+ T cell recognition of M2 but not M1-like macrophagesiScienceood from healthy volunteers with latent Mtb infection University Hospitals IRB protocol N/A PBMCs from leukapheresis product collected from healthy, CMV seropositive volunteers Purchased from AllCells, Inc. N/A Bronchoalveolar Lavage Samples isolated from healthy volunteers Obtained by Dr. Richard Silver, CWRU School of Medicine Ref. 92 ------------------------- Chemicals, pepti;PBMCs from leukapheresis product collected from healthy, CMV seropositive volunteers Purchased from AllCells, Inc. N/AFull Paper
2023Faccioli, LAP;Cetin, Z;Kocas-Kilicarslan, ZN;Ortiz, K;Sun, Y;Hu, Z;Kurihara, T;Tafaleng, EN;Florentino, RM;Wang, Z;Xia, M;Miedel, MT;Taylor, DL;Behari, J;Ostrowska, A;Constantine, R;Li, A;Soto-Gutierrez, A;Evaluation of Human Hepatocyte Drug Metabolism Carrying High-Risk or Protection-Associated Liver Disease Genetic VariantsInternational journal of molecular sciences37686209Metabolic-dysfunction-associated steatotic liver disease (MASLD), which affects 30 million people in the US and is anticipated to reach over 100 million by 2030, places a Save Cached;(CYP) enzymes, polymorphic sites associated with high-risk liver disease progression and protection were determined in 126 human hepatocytes. The cryopreserved primary hepatocytes were obtained from In Vitro ADMET Laboratories Inc. (IVAL, Columbia, MD, USA). The isolations were procured from human liver donors that were rejected for orthotopic liver transplantation. Liver tissue was equally distr;Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.||Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, PA 15261, USA.++Department of Statistics, University of Pittsburgh, Pittsburgh, PA 15213, USA.++Drug Discovery Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.||Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA.++Drug Discovery Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.||Department of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA 15260, USA.++Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, PA 15261, USA.||Department of Statistics, University of Pittsburgh, Pittsburgh, PA 15213, USA.||Drug Discovery Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.++Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, PA 15261, USA.||Department of Medicine, Division of Gastroenterology, Hepatology, and Nutrition, University of Pittsburgh Medical Center, Pittsburgh, PA 15213, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.||Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, PA 15261, USA.++Discovery Life Sciences, Huntsville, AL 35806, USA.++Discovery Life Sciences, Huntsville, AL 35806, USA.++Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.||Pittsburgh Liver Research Center, Human Synthetic Liver Biology Core, University of Pittsburgh, Pittsburgh, PA 15261, USA.||Drug Discovery Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.||McGowan Institute for Regenerative Medicine, Pittsburgh, PA 15219, USAFull Paper
2023Wisdom, KM;Suijker, J;Van den Broek, L;Sridharan, B;Grandhi, TSP;Cheng, A;Lamb, M;Titus, SA;Poore, D;Shah, N;Cheng, SH;Kim, E;Griffin, S;Ekert, J;Lung tumor microphysiological system with 3D endothelium to evaluate modulators of T-cell migrationALTEX37422924Primary human T-Cells (AllCells, StemExpress, or BioIVT) were thawed in one of the , T-cells were obtained directly from AllCells and shipped to the MIMETAS research SaveFull Paper
2023Chiang, YP;Li, Z;He, M;Jones, Q;Pan, M;Han, X;Jiang, XC;Sphingomyelin synthase-related protein SMSr is a phosphatidylethanolamine phospholipase C that promotes nonalcoholic fatty liver diseaseThe Journal of biological chemistry37586586PE-PLC and PE levels in NASH patients. Human liver microarrays with normal and NASH sections were purchased from Sekisui XenoTech, LLC. Human female NASH plasma and control plasma were purchased from Discovery Life Sciences. A and B, SMSr immunostaining was performed on liver sections from human NASH patients and controls. Quantification was shown by fluorogram. C and D, NASH patient and control pFull Paper
2023Rong, Y;Torres-Luna, C;Tuszynski, G;Siderits, R;Chang, FN;Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1)Cancers37444514In brief, tissue staining was performed by Discovery Life Sciences (Newtown, PA, USA) according to standard procedures using optimized antibody concentration and Save CachedFull Paper
2023Lam, GT;Sorvina, A;Martini, C;Prabhakaran, S;Ung, BS;Lazniewska, J;Moore, CR;Beck, AR;Hopkins, AM;Johnson, IRD;Caruso, MC;Hickey, SM;Brooks, RD;Jackett, L;Karageorgos, L;Foster-Smith, EJ;Malone, V;Klebe, S;O'Leary, JJ;Brooks, DA;Logan, JM;Altered endosomal-lysosomal biogenesis in melanomaNeoplasia (New York, N.Y.)37562257ssue samples, including three normal skin samples and 23 biopsies from patients with melanoma (14 of which have normal adjacent regions), which were obtained from BioIVT (Michigan, United States) and Discovery Life Sciences (California, United States), with legal informed consent and relevant clinical information. Tumor stage was classified according to the American Joint Committee on Cancer and UFull Paper
2023Arakawa, M;Sakamoto, Y;Miyagawa, Y;Nito, C;Takahashi, S;Nitahara-Kasahara, Y;Suda, S;Yamazaki, Y;Sakai, M;Kimura, K;Okada, T;iPSC-derived mesenchymal stem cells attenuate cerebral ischemia-reperfusion injury by inhibiting inflammatory signaling and oxidative stressMolecular therapy. Methods & clinical development37637385lates coated with Matrigel (Corning, Corning, NY, USA). The BMMSCs were provided by Kaneka (Tokyo, Japan), and the manufacturing process of BMMSCs was as follows. Human bone marrow was purchased from AllCells (Emeryville, CA, USA); 50 mL of bone marrow was diluted 4-fold with minimum essential medium α (MEM α) (Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBSFull Paper
2023Xu, L;Lahiri, P;Skowronski, J;Bhatia, N;Lattanzi, A;Porteus, MH;Molecular dynamics of genome editing with CRISPR-Cas9 and rAAV6 virus in human HSPCs to treat sickle cell diseaseMolecular therapy. Methods & clinical development37637384cultured as previously described.5,20 Human CB-CD34+ HSPCs were generously provided by the Binns Program for Cord Blood Research at Stanford University. Plerixafor mPB-CD34+ HSPCs were purchased from AllCells (Alameda, CA, USA) or received from the Stanford LCGM Facility. The cells were thawed per the manufacturer’s instructions and were cultured at 37°C, 5% CO2, and 5% O2 for 48 h (48-h pre-sFull Paper
2023Murray, J;Einhaus, T;Venkataraman, R;Radtke, S;Zhen, A;Carrillo, MA;Kitchen, SG;Peterson, CW;Kiem, HP;Efficient manufacturing and engraftment of CCR5 gene-edited HSPCs following busulfan conditioning in nonhuman primatesMolecular therapy. Methods & clinical development37575091ng Container (Thermo Fisher Scientific, Waltham, MA) and stored at −80° for up to 2 weeks until infusion. CFC assays A total 1,000 to 1,200 enriched cells were seeded into 3.5-mL ColonyGEL 1402 (ReachBio, Seattle, WA) in 35-mm petri dishes. Hematopoietic colonies were scored after 12 to 14 days. Arising colonies were identified as colony-forming unit (CFU) granulocyte (CFU-G), CFU macrophageFull Paper
2023Petty, N;Radtke, S;Fields, E;Humbert, O;Llewlyn, M;Laszlo, G;Zhu, H;Jerome, K;Walter, R;Kiem, H;Efficient Long-Term Multilineage Engraftment of CD33-Edited Hematopoietic Stem/Progenitor Cells in Nonhuman PrimatesMolecular Therapy - Methods & Clinical DevelopmentCurrent immunotherapeutic targets are often shared between neoplastic and normal hematopoietic stem/progenitor cells (HSPCs), leading to unwanted on-target, off- Save;For CFC assays, 1000 to 1200 sorted cells were seeded into 3.5-mL ColonyGEL 1402 (ReachBio, 305 Seattle, WA). Hematopoietic colonies were scored after 12 to 14 days. Arising colonies were 306 identFull Paper
2023Boccacci, Y;Dumont, N;Doyon, Y;Laganière, J;Accessory-cell-free differentiation of hematopoietic stem and progenitor cells into mature red blood cellsCytotherapy37598334Full Paper
2023Rathore, AS;Gupta, KK;Govindaraj, SK;Ajmani, A;Arivalagan, J;Anto, RJ;Kalishwaralal, K;Chandran, SA;Targeting BRF2: insights from in silico screening and molecular dynamic simulationsJournal of biomolecular structure & dynamics37705251School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, India.++School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, India.++School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, India.++Division of Cancer Research, Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, India.++Discovery Life Sciences, Malden, MA, USA.++Division of Cancer Research, Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, India.++Division of Cancer Research, Rajiv Gandhi Centre for Biotechnology (RGCB), Thiruvananthapuram, India.++School of Chemical and Biotechnology, SASTRA Deemed to be University, Thanjavur, IndiaFull Paper
2023Mwebaza, I;Shaw, R;Li, Q;Fletcher, S;Achkar, JM;Harding, CV;Carpenter, SM;Boom, WH;Impact of Mycobacterium tuberculosis Glycolipids on the CD4+ T Cell-Macrophage Immunological SynapseJournal of immunology (Baltimore, Md. : 1950)37695687+ T cells and monocytes were also obtained from three adult donors by leukapheresis from AllCells (Almeda, CA). In both cases, all samples were deidentified, and the SaveFull Paper
2023Gao, J;Ren, S;Choonoo, G;Chen, G;Frleta, D;Zhong, J;Gupta, N;Sharma, P;Oyejide, A;Atwal, GS;Macdonald, L;Murphy, A;Kuhnert, F;Microenvironment-dependent growth of Sezary cells in humanized IL-15 miceDisease models & mechanisms37718909Table 1) or healthy control individuals were purchased from Discovery Life Sciences. 5x105 to 1x106 thawed total PBMC cells were intravenously transplanted into sub- SaveFull Paper
2023Alluhayb, AH;Severance, C;Hendry-Hofer, T;Bebarta, VS;Logue, BA;Concurrent determination of cyanide and thiocyanate in human and swine antemortem and postmortem blood by high-performance liquid chromatography-tandem mass spectrometryAnalytical and bioanalytical chemistry37712953The blood was collected by Discovery Life Sciences (Huntsville, Alabama, USA) and shipped on dry ice to the Department of Chemistry and Biochemistry at South Dakota SaveFull Paper
2023Zhuo, X;Howell, BA;Shen, H;Woodhead, JL;Mosure, K;Zhang, Y;Scialis, RJ;Iyer, R;Sun, Y;Boy, KM;Lentz, KA;Denton, RR;Soars, MG;Johnson, BM;Humphreys, WG;Mechanistic investigation of liver injury induced by BMS-932481, an experimental ɣ-secretase modulatorToxicological sciences : an official journal of the Society of Toxicology37261863Primary cryoplateable human hepatocytes (In Vitro ADMET Laboratories, Malden, Massachusetts) were plated on Seahorse 96-well plates (Agilent, Santa Clara, California SaveFull Paper
2023Lam, J;Yu, J;Lee, B;Campagna, C;Yoo, S;Baek, K;Jeon, NL;Sung, KE;Characterizing On-Chip Angiogenesis Induction in a Microphysiological System as a Functional Measure of Mesenchymal Stromal Cell BioactivityAdvanced biology37409400B16; Frederick, MD) at passage 1 (P1), Lonza (8F560, 167 696; Walkersville, MD) at passage 2 (P2), and AllCells (PCBM1632, PCBM1641; Alameda, CA) at P2 (see Figure 3 for donor specifications). MSFull Paper
2023Morsczeck, C;Pieles, O;Beck, HC;Analysis of the phosphoproteome in human dental follicle cells during osteogenic differentiationEuropean journal of oral sciences37664892Human DFCs were obtained commercially (AllCells). The standard culture medium was Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich) supplemented with 10% fetal bovine serum (FBS; Sigma-Aldrich) and 100 μg mLFull Paper
2023Rüschoff, J;Schildhaus, HU;Rüschoff, JH;Jöhrens, K;Bocker-Edmonston, T;Dietmaier, W;Bläker, H;Baretton, G;Horst, D;Dietel, M;Hartmann, A;Klauschen, F;Merkelbach-Bruse, S;Stenzinger, A;Schöniger, S;Tiemann, M;Weichert, W;Büttner, R;[Testing deficient mismatch repair and microsatellite instability : A focused update. German version]Pathologie (Heidelberg, Germany)37548948Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland. josef.rueschoff@dls.com.++Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland.++Institut für Pathologie und Molekularpathologie, Universitätsspital Zürich, Zürich, Schweiz.++Institut für Pathologie, Universitätsklinikum Carl Gustav Carus Dresden, Dresden, Deutschland.++Department of Pathology, Cooper University Health Care, Camden, NJ, USA.++Institut für Pathologie/Zentrum für molekularpathologische Diagnostik, Universität Regensburg, Regensburg, Deutschland.++Institut für Pathologie, Universitätsklinikum Leipzig, Leipzig, Deutschland.++Institut für Pathologie, Universitätsklinikum Carl Gustav Carus Dresden, Dresden, Deutschland.++Institut für Pathologie, Charité - Universitätsmedizin Berlin, Campus Mitte, Berlin, Deutschland.++Institut für Pathologie, Charité - Universitätsmedizin Berlin, Campus Mitte, Berlin, Deutschland.++Pathologisches Institut, Universität Erlangen-Nürnberg, Erlangen, Deutschland.++Pathologisches Institut, Ludwig-Maximilians-Universität München, München, Deutschland.++Institut für Pathologie, Universitätsklinikum Köln, Köln, Deutschland.++Pathologisches Institut, Universitätsklinikum Heidelberg, Heidelberg, Deutschland.++Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland.++Institut für Hämatopathologie Hamburg, Hamburg, Deutschland.++Institut für Pathologie, Technische Universität München, München, Deutschland.++Institut für Pathologie, Universitätsklinikum Köln, Köln, DeutschlandFull Paper
2023Stark, GF;Martin, RM;Smith, LE;Wei, B;Hellweger, FL;Bullerjahn, GS;McKay, RML;Boyer, GL;Wilhelm, SW;Cool temperature acclimation in toxigenic Microcystis aeruginosa PCC 7806 and its non-toxigenic mutantbioRxiv : the preprint server for biology37693631esent. Final RNA concentrations were determined using a high-sensitivity Qubit RNA assay per manufacturer’s instructions (Invitrogen). RNA sequencing and sequence analyses RNA samples were sent to Discovery Life Sciences (previously Hudson Alpha, Huntsville, AL) where sample library prep and Illumina Novaseq 6000 platform sequencing (50 million reads, 150 bp, paired end) were performed after riFull Paper
2023Favaro, P;Glass, DR;Borges, L;Baskar, R;Reynolds, W;Ho, D;Bruce, T;Tebaykin, D;Scanlon, VM;Shestopalov, I;Bendall, SC;Unravelling human hematopoietic progenitor cell diversity through association with intrinsic regulatory factorsbioRxiv : the preprint server for biology37693547All the primary samples were deindentified and purchased from suppliers of healthy donors (AllCells and StemExpress). The CD34-enriched cells (CliniMACS, Miltenyi) SaveFull Paper
2023Banerjee, P;Diniz, WJS;Dyce, PW;Dataset for miRNA expression analysis in the peripheral white blood cells of beef heifers at weaningData in brief37680347ution for the final library was assessed by Agilent Bioanalyzer high-sensitivity DNA assay (Agilent, Santa Clara, CA, United States). The quality-checked libraries were sequenced using NextSeq 500 at Discovery Life Sciences (Hudson Alpha Institute of Biotechnology, Huntsville, AL, United States). Single-end 50 bp data was generated for each sample. 4.4 Data Analysis After sequencing, the qualityFull Paper
2023Fernando, V;Zheng, X;Sharma, V;Furuta, S;Reprogramming of breast tumor-associated macrophages with modulation of arginine metabolismbioRxiv : the preprint server for biology37662241btained from Karmanos Cancer Institute (Detroit, MI) under Material Transfer Agreement. Peripheral blood mononuclear cells (PBMCs) were obtained from Stemcell Technologies (Vancouver, BC, Canada) and AllCells LLC (Alameda, CA). Cell culture & Reagents THP-1 cells were maintained at a density of 1×106 cells/ml in RPMI 1640 Medium (ThermoFisher, Waltham, MA, Cat. No. 11835055) supplemented withFull Paper
2023Murgo, E;De Santis, E;Sansico, F;Melocchi, V;Colangelo, T;Padovano, C;Colucci, M;Carbone, A;Totti, B;Basti, A;Gottschlich, L;Relogio, A;Capitanio, N;Bianchi, F;Mazzoccoli, G;Giambra, V;The circadian clock circuitry modulates leukemia initiating cell activity in T-cell acute lymphoblastic leukemiaJournal of experimental & clinical cancer research : CR37620852Interestingly, we found that the addition of recombinant hIL20 to CLOCK-overexpressing T-ALLcells induced greater expansion with respect to mock treated cells, as well Save CachedFull Paper
2023Yadavilli, S;Waight, JD;Brett, S;Bi, M;Zhang, T;Liu, YB;Ellis, C;Turner, DC;Hahn, A;Shi, H;Seestaller-Wehr, L;Jing, J;Xie, Q;Shaik, JS;Ji, X;Gagnon, R;Fieles, W;Hook, L;Grant, S;Hopley, S;DeYoung, MP;Blackwell, C;Chisamore, M;Biddlecombe, R;Figueroa, DJ;Hopson, CB;Srinivasan, R;Smothers, J;Maio, M;Rischin, D;Olive, D;Paul, E;Mayes, PA;Hoos, A;Ballas, M;Activating Inducible T-cell Costimulator Yields Antitumor Activity Alone and in Combination with Anti-PD-1 Checkpoint BlockadeCancer research communications37593752, 6-8-week-old female NSG mice (The Jackson Laboratory, RRID:IMSR_JAX:005557) were engrafted intravenously with 20×106 human PBMC (single donor; AllCells) Save;- Fresh PBMC for NSG TM mice engrafting were purchased from AllCells. with 20 × 10 6 human PBMC (single donor; AllCells) when tumors were approximately 100 mm 3 . SaveFull Paper
2023Hemken, P;Qin, X;Sokoll, L;Jackson, L;Feng, F;Li, P;Gawel, S;Tu, B;Lin, Z;Hartnett, J;Hawksworth, D;Tieman, B;Yoshimura, T;Kinukawa, H;Ning, S;Liu, E;Meng, F;Chen, F;Miao, J;Mi, X;Tong, X;Chan, D;Davis, G;Validation of the Novel GLAS Algorithm for Early Detection of Liver Fibrosis and Cirrhosis Based on GP73, LG2m, Age, and SexResearch Squarex, race/ethnicity, and etiology of liver disease. An additional set of serum samples (collectively referred to as the Western Vendor Cohort, WVC) were purchased from BioIVT (Wesbury, NY), Biomex GmbH (Heidelberg, Germany), Discovery Life Sciences (Huntsville, AL), and ProMedDx (Norton, MA). These three sample sets (JHU/UTSMC/WVC) were combined to develop and trainFull Paper
2023Micco, R;Volpe, L;Vacca, R;Midena, F;Tavella, T;Naldini, M;Barcella, M;Giannetti, K;Ferrari, S;Beretta, S;Farina, G;Alessandrini, L;Santoro, A;Carsana, E;Varesi, A;Gilioli, D;Conti, A;Merelli, I;Gentner, B;Naldini, L;p38 MAPK fuels proliferation stress and DNA damage impairing the functionality of genetically engineered hematopoietic stem and progenitor cellsResearch SquareG-CSF or G-CSF + Plerixafor mPB CD34+ HSPCs were purified in-house with the CliniMACS 719 CD34 Reagent System (Miltenyi Biotec) from Mobilized Leukopak (AllCells) according to the TIGET720 HPCT protocol approved by OSR Ethical Committee and followingFull Paper
2023Bedard, P;Hernando-Calvo, A;Yang, C;Vila-Casadesús, M;Han, M;Liu, A;Berman, H;Spreafico, A;Razak, A;Lheureux, S;Hansen, A;Giacco, D;Matito, J;Pugh, T;Bratman, S;Aleshin, A;Berche, R;Saavedra, O;Garralda, E;Elston, S;Siu, L;Ohashi, P;Vivancos, A;External validation of the VIGex gene-expression signature as a novel predictive biomarker for immune checkpoint treatmentResearch SquareThe level of PD-L1 staining is reported by Qualtek as a modi ed proportion score (MPS, range 0-100), indicating the percentage of PD-L1-expressing tumor cells and SaveFull Paper
2023Biffi, A;Montepeloso, A;Mattioli, D;Pellin, D;Peviani, M;Genovese, P;Targeted gene addition at the CX3CR1 locus of human HSPCs improves their ability to repopulate the myeloid CNS compartment and drives lineage specific and robust transgene expression in their CNS progenyResearch SquareCommercial cord blood CD34+ human HSPCs were purchased frozen from AllCells and were thawed using Thymocyte Thawing Media (TTM) medium, modified from 40. SaveFull Paper
2023Schlabach, M;Lin, S;Collester, Z;Wrocklage, C;Shenker, S;Calnan, C;Xu, T;Gannon, H;Williams, L;Thompson, F;Dunbar, P;LaMothe, R;Garrett, T;Colletti, N;Hohmann, A;Tubo, N;Bullock, C;Le Mercier, I;Sofjan, K;Merkin, J;Keegan, S;Kryukov, G;Dugopolski, C;Stegmeier, F;Wong, K;Sharp, F;Cadzow, L;Benson, M;Rational design of a SOCS1-edited tumor infiltrating lymphocyte therapy for solid tumors using CRISPR/Cas9 screensbioRxivCell therapies such as Tumor Infiltrating Lymphocyte (TIL) therapy have shown promise in the treatment of patients with refractory solid tumors, with improvement in Save;- Dissociated melanoma tumors from donor 110005746 were obtained from Conversant Bio and seeded at 1x56 cells/ml in TIL-CM (RPMI 1640 + 10% HI HS, 1X HEPES, SaveFull Paper
2023Chu, S;Soupene, E;Wienert, B;Yin, H;Sharma, D;Jia, K;Homma, S;Hampton, J;Gardner, J;Conklin, B;MacKenzie, T;Porteus, M;Cromer, M;Dual α-globin and truncated erythropoietin receptor knock-in restores hemoglobin production in α-thalassemia major-derived hematopoietic stem and progenitor cellsbioRxivAlpha-thalassemia is an autosomal recessive monogenic disease with increasing worldwide prevalence. The molecular basis is due to mutation or deletion of one or more SaveFull Paper
2023Sherman, M;Michalski, J;Das, S;Yang, H;Barnoy, S;Chandrasekaran, L;Venkatesan, M;Ernst, R;Targeted lipid A modification ofShigellavaccine strains reduced endotoxicity without compromising immunogenicity or invasivenessbioRxivPeripheral blood monocytes (PBMCs) obtained from 4 independent donors (AllCells), were 730 prepared by two washes of warm cRPMI and seeded at 5 x 105 cells/well SaveFull Paper
2023Yulyaningsih, E;Suh, J;Fanok, M;Chau, R;Solanoy, H;Takahashi, R;Bakardjiev, A;Becerra, I;Benitez, N;Chiu, C;Davis, S;Dowdle, W;Earr, T;Estrada, A;Gill, A;Ha, C;Haddick, P;Henne, K;Larhammar, M;Leung, A;Maciuca, R;Memarzadeh, B;Nguyen, H;Nugent, A;Osipov, M;Ran, Y;Rebadulla, K;Roche, E;Sandmann, T;Wang, J;Lewcock, J;Scearce-Levie, K;Kane, L;Sanchez, P;DNL343 is an investigational CNS penetrant eIF2B activator that prevents and reverses the effects of neurodegeneration caused by the Integrated Stress ResponsebioRxivnts were obtained under a material transfer agreement with Baylor Scott & White Research Institute (Dallas, TX). Samples were collected 15-25 years prior to analysis. Plasma EDTA from healthy controls obtained from BioIVT, Allcells, and Denali Phase 1 clinical trial: NCT04268784. 885 CSF from healthy controls obtained from Biochemed and DFull Paper
2023Golden, A;Slater, H;Jang, I;Walke, S;Phan, T;Bizilj, G;Rashid, A;Barney, B;Domingo, G;Analytical sensitivity analysis and clinical impact modeling of Rapigen rapid diagnostics tests for malariamedRxivAll clinical positive samples from Discovery Life Sciences used in panels were confirmed 99 positive for Plasmodium monoinfection by photo-induced electron transfer SaveFull Paper
2023Camarena, J;Luna, S;Hampton, J;Majeti, K;Charlesworth, C;Soupene, E;Sheehan, V;Cromer, M;Porteus, M;Using human genetics to develop strategies to increase erythropoietic output from genome-edited hematopoietic stem and progenitor cellsbioRxivHuman genetic polymorphisms result in a diversity of phenotypes. Some sequences are pathologic and lead to monogenic diseases, while others may confer beneficial SaveFull Paper
2023Byrne, A;Le, D;Sereti, K;Menon, H;Patel, N;Lund, J;Xavier-Magalhaes, A;Shi, M;Sterne-Weiler, T;Modrusan, Z;Stephenson, W;Single-cell long-read targeted sequencing reveals transcriptional variation in ovarian cancerbioRxivOvarian cancer dissociated tumor cells were purchased through Discovery Life Sciences (Huntsville, AL). Both samFull Paper
2023Fong, H;Mendel, M;Jascur, J;Najmi, L;Kim, K;Lew, G;Garimalla, S;Schock, S;Hu, J;Villegas, A;Conway, A;Fontenot, J;Zompi, S;A Serum- and Feeder-Free System to Generate CD4 and Regulatory T Cells from Human iPSCsbioRxivCD4 T cells were isolated from mobilized 413 leukapheresis material from a healthy male donor (AllCells, LLC) with informed written consent. 414 The raw material was SaveFull Paper
2023Machado, A;Pereira, I;Pereira, J;Maltez, L;Brandão, A;Alvites, R;Sousa, A;Branquinho, M;Caseiro, A;Pedrosa, S;Maurício, A;Pires, I;Prada, J;Santos, J;Gama, M;Dextrin hydrogel loaded with a macroporous Bonelike® scaffold and dental pulp stem cells for critical-sized defect repairMaterialiaCells were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01) and cultured under standard conditiFull Paper
2023Freitas, AVDA;eVOLVER-construção e automação de plataforma de cultura celular e utilização de métodos alternativos para a medição de pHThesisFonte: https://www.mouser.ca/Product Detail/Qualtek/FMA1- 09225WBHW12?qs=T3oQrply3y%2FWD TeWGhtP%252BA%3D%3D, adaptadoFull Paper
2022Catapult, UK;Karydis, A;Kazanis, L;Table of Contents2022 IEEE Future Networks World Forum (FNWF)(Ericsson Inc., Canada)Federated Machine Learning Through edge Ready Architectures with Privacy Preservation as aService 347 Konstantinos Koutsopoulos (Qualtek SaveFull Paper
2023Follmann, M;Surveillance von arboviralen Infektionen und Malaria in einem Distriktkrankenhaus in Nord-Tansania mittels molekularbiologischer Diagnostik und POC-SchnelltestsThesisRift-Valley-Virus (RVFV) Robert Koch Institut, Berlin Inaktivierter Zellkulturüberstand Plasmodium falciparum Discovery Life Sciences P.fal DLS16-11865 VB P.viv DLS15-08486 Serum P.mal Serum Vollblut oder Serum von Malariapatienten, mikroskopisch überprüfte Proben mit hoher ErregerlastFull Paper
2023Murach, A;Marquez, G;Tonn, K;Vormbaum, J;In-Situ Mechanical TesterThesis2 131000 Bearal jack plug 1 Tensility Internation Corp CONN JACK PNL MNT 5.5X2.1MM ISO Digikey $4.97 $4.97 2 131000 AC Power Cord 1 CNC Tech CORD 5/15P - C13 18AWG BLACK 3FT Digikey $9.52 $9.52 2 Heat Shrink 1 Qualtek HEATSHRINK 3/16" X 4' BLACK Digikey $0.81 $0.81Full Paper
2023Houk, BE;Alvey, CW;Visswanathan, R;Kirkovsky, L;Matschke, KT;Kimoto, E;Ryder, T;Obach, RS;Aurora Signalingaurorasignaling.com, Illinois), radioimmunoprecipitation assay buffer from TEKnova (Hollister, California), and cryopreserved hepatocytes (lot HH1025) from In Vitro ADMET laboratories ( SaveFull Paper
2023Sherman, ME;Bacterial Enzymatic Combinatorial Chemistry (BECC) Enabled Targeted Lipid a Modification of Shigella Vaccine Strains to Reduce Endotoxicity Without Compromising Immunogenicity or InvasivenessThesisPeripheral blood monocytes (PBMCs) were obtained from 4 independent donors (AllCells), rapidly thawed in a 37°C water bath, and diluted with 500 µL of cRPMI, dropwise. Cells were harvested by centrifugation (800 RPM, 5 minutes) aFull Paper
2023Boccacci, Y;Modélisation in vitro de variants génétiques dans des globules rouges dérivés de cellules souches hématopoïétiques avec CRISPR-Cas9ThesisPurified mobilized peripheral blood stem cells (mPBSC) were purchased from AllCells (Alameda, CA, USA). Cord blood (CB) units were collected at the Centre Hospitalier Universitaire Saint-François d’Assise (Québec, QC, Canada), after informed consent of participFull Paper
2023Chu, Q;Perrone, F;Greillier, L;Tu, W;Piccirillo, MC;Grosso, F;Lo Russo, G;Florescu, M;Mencoboni, M;Morabito, A;Cecere, FL;Ceresoli, GL;Dawe, DE;Zucali, PA;Pagano, M;Goffin, JR;Sanchez, ML;Gridelli, C;Zalcman, G;Quantin, X;Westeel, V;Gargiulo, P;Delfanti, S;Tu, D;Lee, CW;Leighl, N;Sederias, J;Brown-Walker, P;Luo, Y;Lantuejoul, S;Tsao, MS;Scherpereel, A;Bradbury, P;Laurie, SA;Seymour, L;Pembrolizumab plus chemotherapy versus chemotherapy in untreated advanced pleural mesothelioma in Canada, Italy, and France: a phase 3, open-label, randomised controlled trialLancet (London, England)37931632Background Pleural mesothelioma usually presents at an advanced, incurable stage. Chemotherapy with platinum-pemetrexed is a standard treatment. We hypothesised SaveFull Paper
2023Ackerman, L;Acloque, G;Bacchelli, S;Schwartz, H;Feinstein, BJ;La Stella, P;Alavi, A;Gollerkeri, A;Davis, J;Campbell, V;McDonald, A;Agarwal, S;Karnik, R;Shi, K;Mishkin, A;Culbertson, J;Klaus, C;Enerson, B;Massa, V;Kuhn, E;Sharma, K;Keaney, E;Barnes, R;Chen, D;Zheng, X;Rong, H;Sabesan, V;Ho, C;Mainolfi, N;Slavin, A;Gollob, JA;IRAK4 degrader in hidradenitis suppurativa and atopic dermatitis: a phase 1 trialNature medicine37957373For selectivity analysis, isolated human PBMCs (AllCells) were treated with KT-474 at 300 nM in RPMI 1640 media supplemented with 10% FBS and penicillin- SaveFull Paper
2023Baumgartner, CK;Ebrahimi-Nik, H;Iracheta-Vellve, A;Hamel, KM;Olander, KE;Davis, TGR;McGuire, KA;Halvorsen, GT;Avila, OI;Patel, CH;Kim, SY;Kammula, AV;Muscato, AJ;Halliwill, K;Geda, P;Klinge, KL;Xiong, Z;Duggan, R;Mu, L;Yeary, MD;Patti, JC;Balon, TM;Mathew, R;Backus, C;Kennedy, DE;Chen, A;Longenecker, K;Klahn, JT;Hrusch, CL;Krishnan, N;Hutchins, CW;Dunning, JP;Bulic, M;Tiwari, P;Colvin, KJ;Chuong, CL;Kohnle, IC;Rees, MG;Boghossian, A;Ronan, M;Roth, JA;Wu, MJ;Suermondt, JSMT;Knudsen, NH;Cheruiyot, CK;Sen, DR;Griffin, GK;Golub, TR;El-Bardeesy, N;Decker, JH;Yang, Y;Guffroy, M;Fossey, S;Trusk, P;Sun, IM;Liu, Y;Qiu, W;Sun, Q;Paddock, MN;Farney, EP;Matulenko, MA;Beauregard, C;Frost, JM;Yates, KB;Kym, PR;Manguso, RT;The PTPN2/PTPN1 inhibitor ABBV-CLS-484 unleashes potent anti-tumour immunityNature37794185ces regulations and the International Conference on Harmonisation Guidelines. All human research participants signed informed consent forms. Blood samples from patients with cancer were acquired from Discovery Life Sciences. Data from whole blood showing occasional overt clotting after overnight culture were excluded. pSTAT flow cytometry analyses Heparinized blood samples were treated with AC484Full Paper
2023Tsuchida, CA;Brandes, N;Bueno, R;Trinidad, M;Mazumder, T;Yu, B;Hwang, B;Chang, C;Liu, J;Sun, Y;Hopkins, CR;Parker, KR;Qi, Y;Hofman, L;Satpathy, AT;Stadtmauer, EA;Cate, JHD;Eyquem, J;Fraietta, JA;June, CH;Chang, HY;Ye, CJ;Doudna, JA;Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cellsCell37794590cells were originally derived from a female donor. Primary adult peripheral blood mononuclear cells Primary adult peripheral blood mononuclear cells (PBMCs) were obtained as cryopreserved vials from Allcells Inc. CD3+ T cells were isolated from PBMCs using EasySep Human T Cell Isolation Kits (StemCell Technologies) according to the manufacturer’s instructions. Isolated CD3+ T cells were cultureFull Paper
2023Ligero, M;El Nahhas, O;Prior Palomares, O;Navarro, V;Sansano, I;Serna, G;Mauchanski, S;Toledo, R;Dienstmann, R;Ramon y Cajal, S;Garralda, E;Nuciforo, P;Kather, J;Perez Lopez, R;176P Enhancing immunotherapy response prediction via multimodal integration of radiology and pathology deep learning modelsAnnals of OncologyPersonal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. J.N. Kather: Financial Interests, Personal, Invited Speaker: Fresenius, Eisai, MSD; Financial Interests, Personal, Advisory Board: Owkin, DoMore Diagnostics, Panakeia, London, UK. R. Perez Lopez: Financial Interests, Personal, Full or part-time Employment, VHIO staff (team leader of the Radiomics Group): VHIO; Financial Interests, Institutional, Research Grant,Full Paper
2023Jové, M;Braña, I;Oliva Bernal, M;Hernando Calvo, A;Erasun Lecuona, C;Assaf Pastrana, J;Mato-Berciano, A;Maliandi, M;Torres-Manjon, S;Moreno, R;Le, C;Nuciforo, P;Alemany, R;Capella, G;Blasco, C;Cascallo Piqueras, M;Mesia Nin, R;937P Survival outcomes in phase I trial combining VCN-01 and durvalumab (MEDI4736) in subjects with recurrent/metastatic head and neck squamous cell carcinoma refractory to previous immunotherapy treatmentAnnals of Oncologyrsonal, Advisory Board: BAYER, MSD ONCOLOGY; Financial Interests, Personal, Invited Speaker: NOVARTIS; Financial Interests, Personal, Other, Consultant: TARGOS Molecular Pathology. R. Alemany: Financial Interests, Personal, Stocks/Shares: Theriva Biologics; Financial Interests, Institutional, Research Funding, T: Theriva Biologics SL; Financial Interests, Institutional, Speaker, Consultant, Advisor: Theriva Biologics SL. G. Capella: Financial Interests, Personal, Other, Consultancy: THERIVA BIOLOGICS; Financial Interests, Personal, Stocks/Shares: THERIVA BIOLOGICS; NonFinancial Interests, Member of Board of Directors, Chairperson 2022-2024: International Society for the Study of Hereditary Gastrointestinal Cancer; Non-Financial Interests, Member of Board of Directors: FUREGA Fundació Per la Recerca en Gastroenterologia; Non-Financial Interests, Member: ASCO, EACR-ASEICA, EHTG European Hereditary Tumor Group. C. Blasco: Financial IFull Paper
2023Haubner, S;Mansilla-Soto, J;Nataraj, S;Kogel, F;Chang, Q;de Stanchina, E;Lopez, M;Ng, MR;Fraser, K;Subklewe, M;Park, JH;Wang, X;Rivière, I;Sadelain, M;Cooperative CAR targeting to selectively eliminate AML and minimize escapeCancer cell37802054Normal donor whole bone marrow, fresh Allcells N/A Buffy coats New York Blood Center N/A AML patient samples MSKCC IRB# 06-107 and IRB# 14-091 AML patient-derived xenograft modeFull Paper
2023Lorenzen, S;Götze, TO;Thuss-Patience, P;Biebl, M;Homann, N;Schenk, M;Lindig, U;Heuer, V;Kretzschmar, A;Goekkurt, E;Haag, GM;Riera-Knorrenschild, J;Bolling, C;Hofheinz, RD;Zhan, T;Angermeier, S;Ettrich, TJ;Siebenhuener, AR;Elshafei, M;Bechstein, WO;Gaiser, T;Loose, M;Sookthai, D;Kopp, C;Pauligk, C;Al-Batran, SE;AIO and SAKK Study Working Groups, ;Perioperative Atezolizumab Plus Fluorouracil, Leucovorin, Oxaliplatin, and Docetaxel for Resectable Esophagogastric Cancer: Interim Results From the Randomized, Multicenter, Phase II/III DANTE/IKF-s633 TrialJournal of clinical oncology : official journal of the American Society of Clinical Oncology37963317Honoraria: Servier, MSD Oncology, Lilly, Bristol Myers Squibb, Targos GmbH, iOMEDICO, MCI Conventions, AstraZeneca Consulting or Advisory Role: Bristol Myers Squibb (Inst), MSD Oncology (Inst), Lilly, Novartis, Daiichi Sankyo Europe GmbH, Servier, Pierre Fabre, AstraZeneca Research Funding: Taiho Pharmaceutical (Inst), Nordic Group (Inst), MSD Oncology (Inst) Travel, Accommodations, ExpenseFull Paper
2023Sun, Y;Yang, XN;Yang, SS;Lyu, YZ;Zhang, B;Liu, KW;Li, N;Cui, JC;Huang, GX;Liu, CL;Xu, J;Mi, JQ;Chen, Z;Fan, XH;Chen, SJ;Chen, S;Antigen-induced chimeric antigen receptor multimerization amplifies on-tumor cytotoxicitySignal transduction and targeted therapy38062078edia (Thermo Fisher 10743029). All cell lines were cultured at 37?°C, 5% CO2. Human samples The healthy donor peripheral blood mononuclear cells (PBMCs) were obtained from HemaCare Corporation or AllCells. Identification and engineering of the nanobody tandem We immunized two llamas with purified BCMA, yielding a high-volume nanobody phage display library from which we isolated and sequencedFull Paper
2023Nolan-Stevaux, O;Li, C;Liang, L;Zhan, J;Estrada, J;Osgood, T;Li, F;Zhang, H;Case, R;Murawsky, CM;Estes, B;Moore, GL;Bernett, MJ;Muchhal, U;Desjarlais, JR;Staley, BK;Stevens, J;Cooke, KS;Aeffner, F;Thomas, O;Stieglmaier, J;Lee, JL;Coxon, A;Bailis, JM;AMG 509 (Xaluritamig), an Anti-STEAP1 XmAb 2+1 T-cell Redirecting Immune Therapy with Avidity-Dependent Activity Against Prostate CancerCancer discovery37861452ation using STAT-Myco testing (IDEXX Bioanalytics). Effector cells were human T cells (AllCells) or human or cynomolgus monkey PBMCs (SNBL)Full Paper
2023Kwok, SJJ;Forward, S;Fahlberg, MD;Assita, ER;Cosgriff, S;Lee, SH;Abbott, GR;Zhu, H;Minasian, NH;Vote, AS;Martino, N;Yun, SH;High-dimensional multi-pass flow cytometry via spectrally encoded cellular barcodingNature biomedical engineering38036616Anti-CD45 antibodies conjugated to the 14 fluorophores were used to stain freshly thawed human PBMCs (AllCells). Cells (10 6 ) were photobleached in 2 ml of 'wash SaveFull Paper
2023Olson, TS;Walters, MC;Allogeneic haematopoietic stem-cell transplantation versus gene therapy for haemoglobinopathiesThe Lancet. Haematology37793770MCW has received consulting fees from AllCells Incorporated and BioChips Incoroporated; payment or honoraria for lectures from TouchIME; and is on the scientific advisory board for Ensoma Incorporated and the steering committee for Vertex Pharmaceuticals InFull Paper
2023Han, W;Qiu, HY;Sun, S;Fu, ZC;Wang, GQ;Qian, X;Wang, L;Zhai, X;Wei, J;Wang, Y;Guo, YL;Cao, GH;Ji, RJ;Zhang, YZ;Ma, H;Wang, H;Zhao, M;Wu, J;Bi, L;Chen, QB;Li, Z;Yu, L;Mou, X;Yin, H;Yang, L;Chen, J;Yang, B;Zhang, Y;Base editing of the HBG promoter induces potent fetal hemoglobin expression with no detectable off-target mutations in human HSCsCell stem cell37989316processed according to the protocol of Miltenyi CD34 Microbead kit (Miltenyi Biotec). The genotypes of the three b0 /b0 donors as shown: the first donor: aa-4.2/aa, bCD41-42(-TTCT)/bCD41-42(-TTCT); the second donor: aa-3.7/aa, bCD41-42(-TTCT)/bCD41-42(-TTCT); the third donor: aa/a-3.7aHb Westmead, bCD41-42(-TTCT)/bCD41-42(-TTCT). Frozen healthy CD34+ cells were purchased from Allcells and SAILY BIO (China)Full Paper
2023Kassim, A;Walters, M;Eapen, M;Ritzau, N;Smith, M;Solh, M;McKinney, C;Nieder, M;Ross, M;Kent, M;Abusin, G;Mallhi, K;Silva, J;Shaughnessy, P;Kanter, J;Haines, H;Farah, R;Khaled, Y;Abraham, A;Bollard, C;Cooke, K;de La Fuente, J;Hanna, R;Horowitz, M;Jordan, L;Krishnamurti, L;Leifere, E;Mahadeo, K;Shenoy, S;Ritzau, N;DeBaun, M;Brodsky, R;Reduced Intensity Haploidentical Bone Marrow Transplantation in Adults with Severe Sickle Cell Disease: BMT CTN 1507BloodBackground: Allogeneic hematopoietic stem-cell transplantation has curative potential for sickle cell disease (SCD). Event-free survival (EFS) in children with SCD is >90% SaveFull Paper
2023Frangoul, H;Locatelli, F;Sharma, A;Bhatia, M;Mapara, M;Molinari, L;Wall, D;Liem, R;Telfer, P;Shah, A;Cavazzana, M;Corbacioglu, S;Rondelli, D;Meisel, R;Dedeken, L;Lobitz, S;de Montalembert, M;Steinberg, M;Walters, M;Bower, L;Imren, S;Simard, C;Xuan, F;Zhou, W;Morrow, P;Hobbs, W;Grupp, S;Exagamglogene Autotemcel for Severe Sickle Cell DiseaseBloodmaceuticals: Consultancy; BioChip Labs: Consultancy, Other: Medical Director; AllCells, Inc: Consultancy, Other: Medical Director. Bower: Vertex Pharmaceuticals: Current Employment. Imren: Vertex Pharmaceuticals: Current Employment, Current holder of stock options in a privately-held company. Simard: Vertex Pharmaceuticals: Current Employment. Xuan: Vertex Pharmaceuticals: Current Employment. Zhou: Vertex Pharmaceuticals: Current Employment. Morrow: Vertex Pharmaceuticals: Membership on an enFull Paper
2023Hanna, R;Frangoul, H;McKinney, C;Pineiro, L;Mapara, M;Dalal, J;Chang, K;Jaskolka, M;Kim, K;Farrington, D;Wally, M;Mei, B;Lawal, A;Afonja, O;Walters, M;AsCas12a Gene Editing of HBG1/2 Promoters with EDIT-301 Results in Rapid and Sustained Normalization of Hemoglobin and Increased Fetal Hemoglobin in Patients with Severe Sickle Cell Disease and Transfusion-Dependent Beta-ThalassemiaBloodoyment. Walters: AllCells, Inc: Consultancy, Other: Medical Director; BioChip Labs: Consultancy, Other: Medical Director; Vertex Pharmaceuticals: Consultancy; Ensoma, Inc:Full Paper
2023Huang, J;Gu, H;Orton, J;Sedova, M;Marcovitz, A;Burke, J;Brozio, S;Williams, P;Myrand, S;Olowo, N;Broomer, A;Deal, B;Seeger, C;Sadis, S;Rozenzhak, S;Hyland, F;Liu, G;Detection of KMT2A Partial Tandem Duplications ( KMT2A-PTDs) in Healthy Donors Using Next Generation SequencingBloodIntroduction KMT2A ( MLL) fusions are relevant biomarkers in myeloid malignancies. KMT2A-PTD (also called MLL-PTD, partial tandem duplication), a non-fusion gene rearrangement, also occurs and serves as an important biomarker for Menin-inhibitors. Traditionally, KMT2A-PTDs are detected by RT-qPCR (quantitative real-time PCR). Here we report the detection of KMT2A-PTDs in whole blood samples taken from healthy donors using the Oncomine TM Myeloid Assay GX v2, which is a next-generation sequencing (NGS) based assay that enables efficient detection of multiple genetic alterations in parallel. KMT2A-PTD positive clinical research samples were used as a control in this study. This study was initiated based on reports from various healthcare professionals who observed several healthy blood donors identified with KMT2A-PTD using the Oncomine TM Myeloid Assay GX v2. As a result, we conducted tests on healthy controls and compared them with clinical research samples known to have myeloid malignancies to explore this genomic alteration further. Methods We sequenced 20 whole blood samples (total 104 replicates) from healthy donors at 3 different sites of Thermo Fisher Scientific TM (South San Francisco, CA; Guilford, CT; Carlsbad, CA). These whole blood samples were received from Stanford Blood Center or purchased from Discovery Life Sciences. As a control, we also sequenced 20 clinical research samples (total 85 replicates) that are known to have myeloid malignancies in both Sonora Quest Laboratories and at Thermo Fisher Scientific TM South San Francisco site. These samples were run on the Ion Torrent TM Genexus TM Software 6.6 and analyzed using the Oncomine TM Myeloid Assay GX v2 with fusion profiling, where the assay design targets 6 different KMT2A-PTD variants. Results The data set has mean read length 90 - 120bp and total mapped fusion reads 20,000 - 30,000. We have found evidence of KMT2A-PTDs in confirmed positive samples from myeloid malignancies (including: AML, acute myeloid leukemia; MPN, myeloproliferative neoplasms; MDS, myelodysplastic syndromes; CML, chronic myeloid leukemia; CMML, chronic myelomonocytic leukemia; or JMML, juvenile myelomonocytic leukemia) and healthy donor samples. The read counts of PTDs detected in the healthy donor samples are on average about 1/3 of the read counts of PTDs detected in the myeloid positive samples, but the distributions overlap. Approximately 33% of myeloid positive samples have PTD read counts higher than the maximum read counts detected in any of the healthy donor samples. The sequences of KMT2A-PTDs found in both healthy donor samples and myeloid samples were further analyzed in BLAT (BLAST-Like Alignment Tool) to confirm that they match the specific exons on the KMT2A gene. Based on this data, only samples with myeloid malignancies have &gt;2000 reads that support KMT2A-PTDs with this assay. Conclusions We describe the detection of KMT2A-PTDs in a range of clinical research samples, including healthy controls as well as samples with myeloid malignancies. KMT2A-PTDs were detected in healthy control samples at lower levels, however, one third of myeloid samples showed significantly higher KMT2A-PTD read counts compared to healthy controls. This intriguing finding warrants further investigation, as it opens up the possibility of conducting a prospective study to follow up on these individuals and observe if they develop myeloid malignancies. Moreover, this finding presents an opportunity for conducting retrospective studies to explore whether healthy controls previously identified with this alteration several years ago after blood donation were subsequently recorded in the health national system with myeloid malignancies. (For research use only. Not for use in diagnostic procedures.);- These whole blood samples were received from Stanford Blood Center or purchased from Discovery Life Sciences. As a control, we also sequenced 20 clinical research SaveFull Paper
2023Kanter, J;Thompson, A;Kwiatkowski, J;Parikh, S;Mapara, M;Rifkin-Zenenberg, S;Aygun, B;Kasow, K;Gupta, A;Zhang, L;Sheldon-Waniga, E;Gallagher, M;Gruppioni, K;Chawla, A;Elliot, H;Pierciey, F;Walters, M;Tisdale, J;Efficacy, Safety, and Health-Related Quality of Life (HRQOL) in Patients with Sickle Cell Disease (SCD) Who Have Received Lovotibeglogene Autotemcel (Lovo-cel) Gene Therapy: Up to 60 Months of Follow-upBloodEmployment, Current equity holder in private company. Walters: AllCells, Inc: Consultancy, Other: Medical Director; BioChip Labs: Consultancy, Other: Medical Director; Ensoma, Inc: Consultancy; Vertex Pharmaceuticals: ConsultanFull Paper
2023Thompson, A;Olson, T;Walters, M;Porter, J;Schneiderman, J;Hongeng, S;Kulozik, A;Cavazzana, M;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Rasko, J;Yannaki, E;Ali, S;Tao, G;Thakar, H;Deora, A;Gruppioni, K;Colvin, R;Locatelli, F;Kwiatkowski, J;Sustained Efficacy, Safety, and Improved Quality of Life in Adult and Pediatric Patients with Transfusion-Dependent ?-Thalassemia up to 9 Years Post Treatment with Betibeglogene Autotemcel (Beti-cel)Bloodics: Other: DSMB; Vertex Pharmaceuticals: Consultancy, Speakers Bureau. Walters: AllCells, Inc: Consultancy, Other: Medical Director; BioChip Labs: Consultancy, Other: Medical Director; Ensoma, Inc: Consultancy; Vertex Pharmaceuticals: Consultancy. Porter: BMS, Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees; Celgene, bluebird bio, Agios: Consultancy, Honoraria; ProtagFull Paper
2023Kwiatkowski, J;Olson, T;Walters, M;Porter, J;Schneiderman, J;Hongeng, S;Kulozik, A;Cavazzana, M;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Rasko, J;Yannaki, E;Ali, S;Tao, G;Deora, A;Thakar, H;Colvin, R;Locatelli, F;Thompson, A;Improvement in Iron Burden in Patients with Transfusion-Dependent ?-Thalassemia (TDT) Treated with Betibeglogene Autotemcel (Beti-cel) Gene Therapy: Up to 9 Years of Follow-upBloodvately-held company; Vertex Pharmaceuticals: Membership on an entity's Board of Directors or advisory committees; BioLabs, Inc, AllCells, Inc: Consultancy. Porter: BMS, Novartis: Honoraria, Membership on an entity's Board of Directors or advisory committees; Protagonism, VIFOR, Silence Therapeutics, La Jolla Pharmaceuticals: Honoraria; Celgene, bluebird bio, Agios: Consultancy, Honoraria; Silence Therapeutics: Honoraria, Membership on an entity's Board of Directors or advisoFull Paper
2023Janesick, A;Shelansky, R;Gottscho, AD;Wagner, F;Williams, SR;Rouault, M;Beliakoff, G;Morrison, CA;Oliveira, MF;Sicherman, JT;Kohlway, A;Abousoud, J;Drennon, TY;Mohabbat, SH;10x Development Teams, ;Taylor, SEB;High resolution mapping of the tumor microenvironment using integrated single-cell, spatial and in situ analysisNature communications38114474-26 and obtained from Discovery Life Sciences. Corresponding dissociated tumor cells, -07-24 and obtained from Discovery Life Sciences. 5 ?m sections were taken from Save;ple collection Sample #1. A single formalin-fixed, paraffin-embedded (FFPE) breast cancer tissue block (TNM stage T2N1M0, ER?+?/HER2?+?/PR??) was collected on 2021-07-26 and obtained from Discovery Life Sciences. Corresponding dissociated tumor cells, fresh frozen in liquid nitrogen, were also sampled from the same biopsy (patient matched). 5 ?m sections were taken from the FFPE tissuFull Paper
2023Sun, D;Sun, Y;Janezic, E;Zhou, T;Johnson, M;Azumaya, C;Noreng, S;Chiu, C;Seki, A;Arenzana, TL;Nicoludis, JM;Shi, Y;Wang, B;Ho, H;Joshi, P;Tam, C;Payandeh, J;Comps-Agrar, L;Wang, J;Rutz, S;Koerber, JT;Masureel, M;Structural basis of antibody inhibition and chemokine activation of the human CC chemokine receptor 8Nature communications38040762Human colorectal DTC (Discovery Life Sciences) were thawed according to the vendor's protocol. Human PBMCs were isolated by Ficoll gradient centrifugation from buffy SaveFull Paper
2023Schlabach, MR;Lin, S;Collester, ZR;Wrocklage, C;Shenker, S;Calnan, C;Xu, T;Gannon, HS;Williams, LJ;Thompson, F;Dunbar, PR;LaMothe, RA;Garrett, TE;Colletti, N;Hohmann, AF;Tubo, NJ;Bullock, CP;Le Mercier, I;Sofjan, K;Merkin, JJ;Keegan, S;Kryukov, GV;Dugopolski, C;Stegmeier, F;Wong, K;Sharp, FA;Cadzow, L;Benson, MJ;Rational design of a SOCS1-edited tumor-infiltrating lymphocyte therapy using CRISPR/Cas9 screensThe Journal of clinical investigation38099496Cell therapies such as Tumor Infiltrating Lymphocyte (TIL) therapy have shown promise in the treatment of patients with refractory solid tumors, with improvement in Save;- Dissociated melanoma tumors from donor 110005746 were obtained from Conversant Bio and seeded at 1x56 cells/ml in TIL-CM (RPMI 1640 + 10% HI HS, 1X HEPES, SaveFull Paper
2023Zhang, L;Lee, M;Maslov, AY;Montagna, C;Vijg, J;Dong, X;Analyzing somatic mutations by single-cell whole-genome sequencingNature protocols37996541was covered by Einstein-Montefiore Institutional Review Board, Lonza Walkersville Inc., Kerafast Inc., AllCells, and STEMCELL TechnologiesFull Paper
2023Corbacioglu, S;Frangoul, H;Locatelli, F;Hobbs, W;Walters, M;Defining curative endpoints for transfusion-dependent ?-thalassemia in the era of gene therapy and gene editingAmerican journal of hematology38100154armaceuticals Incorporated. William Hobbs is an employee of Vertex Pharmaceuticals Incorporated and holds stock and/or stock options at the company. Mark Walters has served as a consultant for AllCells, Inc., BioChip, Inc., Ensoma, Inc., and Editas Medicine; served on an advisory board for Vertex Pharmaceuticals Incorporated; and holds stock/stock options in Ensoma, IncFull Paper
2023Locatelli, F;Corbacioglu, S;Hobbs, W;Frangoul, H;Walters, MC;Defining curative endpoints for sickle cell disease in the era of gene therapy and gene editingAmerican journal of hematology38010293mployee of Vertex Pharmaceuticals Incorporated and holds stock in the company. Mark Walters has served as a consultant for AllCells, Inc., BioChip, Inc., Ensoma, Inc., and Editas Medicine; served on an advisory board for Vertex Pharmaceuticals Incorporated; and holds stock/stock options in Ensoma, Inc. Open ResearchFull Paper
2023Guo, Q;Zhang, J;Parikh, K;Brinkley, A;Lin, S;Zakarian, C;Pernet, O;Shimizu, S;Khamaikawin, W;Hacke, K;Kasahara, N;An, DS;In vivo selection of anti-HIV-1 gene-modified human hematopoietic stem/progenitor cells to enhance engraftment and HIV-1 inhibitionMolecular therapy : the journal of the American Society of Gene Therapy38087779microbead isolation kit (Miltenyi Biotec, Auburn, CA). Human mPB CD34+ cells were isolated from granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood leukapheresis packs purchased from ALLCELLS (Alameda, CA) using anti-CD34+ monoclonal antibodies (Miltenyi Biotec) and cryopreserved in Bambanker (GC Lymphotec, Tokyo, Japan)Full Paper
2023Wilkerson, AD;Parthasarathy, PB;Stabellini, N;Mitchell, C;Pavicic, PG;Fu, P;Rupani, A;Husic, H;Rayman, PA;Swaidani, S;Abraham, J;Budd, GT;Moore, H;Al-Hilli, Z;Ko, JS;Baar, J;Chan, TA;Alban, T;Diaz-Montero, CM;Montero, AJ;Phase II Clinical Trial of Pembrolizumab and Chemotherapy Reveals Distinct Transcriptomic Profiles by Radiologic Response in Metastatic Triple-Negative Breast CancerClinical cancer research : an official journal of the American Association for Cancer Research37882661Purpose A single arm, Phase II trial of carboplatin, nab-paclitaxel, and pembrolizumab (CNP) in metastatic triple negative breast cancer (mTNBC) was designed to SaveFull Paper
2023Cash, A;de Jager, C;Brickler, T;Soliman, E;Ladner, L;Kaloss, AM;Zhu, Y;Pridham, KJ;Mills, J;Ju, J;Basso, EKG;Chen, M;Johnson, Z;Sotiropoulos, Y;Wang, X;Xie, H;Matson, JB;Marvin, EA;Theus, MH;Endothelial deletion of EPH receptor A4 alters single-cell profile and Tie2/Akap12 signaling to preserve blood-brain barrier integrityProceedings of the National Academy of Sciences of the United States of America37796990proved by Virginia Tech’s IRB #19-470. Legally authorized representatives provided written informed consent. Subjects aged 18 to 50 y had labs drawn at 24, 48, and 72 h following hospital admission. Control samples were purchased to match age, sex, and race (Discovery Life Sciences). ELISAs were conducted on serum samples to quantify Ang2 expression according to the manufacturer’s instructions (Boster Bio)Full Paper
2023Schmidt, F;Fields, HF;Purwanti, Y;Milojkovic, A;Salim, S;Wu, KX;Simoni, Y;Vitiello, A;MacLeod, DT;Nardin, A;Newell, EW;Fink, K;Wilm, A;Fehlings, M;In-depth analysis of human virus-specific CD8+ T cells delineates unique phenotypic signatures for T cell specificity predictionCell reports37837618PBMCs (from whole blood) Discovery Life Sciences N/A PBMCs (from whole blood) San Diego Blood Bank N/A (ContinuedFull Paper
2023Song, J;Lv, H;Liu, B;Hao, M;Taylor, HS;Zhang, X;Li, D;Huang, Y;Let-7 suppresses liver fibrosis by inhibiting hepatocyte apoptosis and TGF-? productionMolecular metabolism37898449111917V1, Lot# HH1161, 35 years old African American male, BMI 32) were purchased from Discovery Life Sciences. Cells were thawed using 37 °C Universal Cryopreservation Recovery Media (UCRM, 81015, Discovery Life Sciences) and seeded in Universal Primary Cell Plating Media (UPCM, 81016, Discovery Life Sciences). 2.7 Transfection of primary hepatocytes For let-7a transfection experiments, cellsFull Paper
2023Copertino, DC;Holmberg, CS;Weiler, J;Ward, AR;Howard, JN;Levinger, C;Pang, AP;Corley, MJ;Dündar, F;Zumbo, P;Betel, D;Gandhi, RT;McMahon, DK;Bosch, RJ;Linden, N;Macatangay, BJ;Cyktor, JC;Eron, JJ;Mellors, JW;Kovacs, C;Benko, E;Bosque, A;Jones, RB;AIDS Clinical Trials Group (ACTG) A5321 Team, ;The latency-reversing agent HODHBt synergizes with IL-15 to enhance cytotoxic function of HIV-specific T cellsJCI insight37581929an Attune NxT Flow Cytometer (Thermo Fisher Scientific) immediately. Data were analyzed using FlowJo software. Infected cell degranulation assay PBMCs from deidentified HLA-B58+ HIV-negative donors (AllCells) were depleted of CD8+ T cells (STEMCELL Technologies, EasySep, catalog 17853) and activated with ImmunoCult Human CD3/CD28 T Cell Activator (STEMCELL Technologies, catalog 10971) (for 72 houFull Paper
2023Cahyanto, A;Rath, P;Teo, TX;Tong, SS;Malhotra, R;Cavalcanti, BN;Lim, LZ;Min, KS;Ho, D;Lu, WF;Rosa, V;Designing Calcium Silicate Cements with On-Demand Properties for Precision EndodonticsJournal of dental research37861249applications. To this end, the cytocompatibility, gene expression, and mineralization potential were tested using dental pulp stem cells (DPSCs, passages 5-8) (DP003F; AllCells). Cells were subcultured in basal growth media (Dulbecco’s modified Eagle’s medium, penicillin-streptomycin, and 10% fetal bovine serum, all from Invitrogen). The cement eluents were prepared following ISO 10993-12:2021 (ISO 2021) in basal growth media (specimen thickness: 1.0 mFull Paper
2023Walseng, E;Wang, B;Yang, C;Patel, P;Zhao, C;Zhang, H;Zhao, P;Mazor, Y;Conformation-selective rather than avidity-based binding to tumor associated antigen derived peptide-MHC enables targeting of WT1-pMHC low expressing cancer cells by anti-WT1-pMHC/CD3 T cell engagersFrontiers in immunology38022650Primary AML cells were purchased as frozen cells from Discovery Life Sciences and used directly in the assay upon thawing and resting overnight. SaveFull Paper
2023Meyer, MA;Dinh, HQ;Alimadadi, A;Araujo, DJ;Chatterjee, N;Gutierrez, NA;Zhu, YP;Hunter, EL;Liang, S;Seumois, G;Kiosses, WB;Catz, SD;Vijayanand, P;Ottensmeier, C;Hedrick, CC;Human CD79b+ neutrophils in the blood are associated with early-stage melanomaFrontiers in immunology38022639Fresh bone marrow collected from anonymous healthy donors was obtained from AllCells, Inc. (Alameda, CA) and shipped overnight on a cold pack. Heparin was used as SaveFull Paper
2023Smith, AJ;Thurman, RE;Zeng, W;Grogan, B;Lucas, S;Gutierrez, G;Heiser, RA;Wo, SW;Blackmarr, A;Peterson, S;Gardai, SJ;Nonfucosylation of an anti-TIGIT antibody enhances Fc?R engagement, driving innate immune activation and antitumor activityFrontiers in immunology38022590For internally generated scRNA-seq data, dissociated primary human lung tumors (DTCs) from three patients were obtained from Discovery Life Sciences (DLS), who Save;- Cryopreserved PBMCs from normal healthy donors (purchased from Astarte and Folio Conversant) were thawed, washed in RPMI 1640, then washed with PBS, stained Save;g from peripheral blood or normal tissues were excluded from the analysis. For internally generated scRNA-seq data, dissociated primary human lung tumors (DTCs) from three patients were obtained from Discovery Life Sciences (DLS), who isolated T cells and performed scRNA sequencing and data analysis using the methodology described in the Supplementary Materials and Methods. Fc?R cell binding AntFull Paper
2023Branella, GM;Lee, JY;Okalova, J;Parwani, KK;Alexander, JS;Arthuzo, RF;Fedanov, A;Yu, B;McCarty, D;Brown, HC;Chandrakasan, S;Petrich, BG;Doering, CB;Spencer, HT;Ligand-based targeting of c-kit using engineered ?? T cells as a strategy for treating acute myeloid leukemiaFrontiers in immunology38022523al blood mononuclear cells (PBMCs) were isolated using a Ficoll-Plaque PLUS density gradient (GE HealthCare, Chicago, IL, USA) and cryopreserved. Alternatively, cryopreserved PBMCs were procured from AllCells (Alameda, CA, USA). T cells were negatively selected from thawed PBMCs using the EasySep Human T cell Isolation Kit (Stemcell Technologies, Vancouver, BC, CA) and stimulated with anti-CD3/CD2Full Paper
2023Noe, P;Wang, JH;Chung, K;Cheng, Z;Field, JJ;Shen, X;Cortesio, CL;Pastuskovas, CV;Phee, H;Tarbell, KV;Egen, JG;Casbon, AJ;Therapeutically targeting type I interferon directly to XCR1+ dendritic cells reveals the role of cDC1s in anti-drug antibodiesFrontiers in immunology37942313Aliquots of single cell suspensions of human dissociated ovarian tumors (serous carcinoma) were purchased from Conversant Biologics (Discovery Life Sciences) and pre- SaveFull Paper
2023Garman, B;Jiang, C;Daouti, S;Kumar, S;Mehta, P;Jacques, MK;Menard, L;Manjarrez-Orduno, N;Dolfi, S;Mukherjee, P;Rai, SC;Lako, A;Koenitzer, JD;David, JM;Comprehensive immunophenotyping of solid tumor-infiltrating immune cells reveals the expression characteristics of LAG-3 and its ligandsFrontiers in immunology37795090Human tumor and whole blood samples were commercially procured from Avaden BioSciences (WA, USA), BioOptions (CA, USA), Discovery Life Sciences (CA, USA), or MT Group (CA, USA). For the CyTOF cohort, a total of 28 tumor samples from 7 tumors types and 7 matched PBMC samples were profiled. For comparison, PBMCs from 10 healthy donors, isolated from whole blood obtained from the BMS internal blood donor program (n=8) or leukopaks commercially procured from Biospecimen Solutions, Inc. (CA, USA) (Full Paper
2023Frey, B;Holzinger, D;Taylor, K;Ehrnhoefer, DE;Striebinger, A;Biesinger, S;Gasparini, L;O'Neill, MJ;Wegner, F;Barghorn, S;Höglinger, GU;Heym, RG;Tau seed amplification assay reveals relationship between seeding and pathological forms of tau in Alzheimer's disease brainActa neuropathologica communications37964332research institute (Arizona, USA), Discovery life sciences (Alabama, USA), Folio Biosciences (by now merged to discovery life sciences). All materials were collected from Save;3, S4), including Tissue Solutions Ltd (Glasgow, UK), Analytical Biological Services Inc. (Delaware, USA), Banner sun health research institute (Arizona, USA), Discovery life sciences (Alabama, USA), Folio Biosciences (by now merged to discovery life sciences). All materials were collected from donors for whom a written informed consent for a brain autopsy and the use of the material and clinical ... 3, S4), including Tissue Solutions Ltd (Glasgow, UK), Analytical Biological Services Inc. (Delaware, USA), Banner sun health research institute (Arizona, USA), Discovery life sciences (Alabama, USA), Folio Biosciences (by now merged to discovery life sciences). All materials were collected from donors for whom a written informed consent for a brain autopsy and the use of the material and clinical ... 3, S4), including Tissue Solutions Ltd (Glasgow, UK), Analytical Biological Services Inc. (Delaware, USA), Banner sun health research institute (Arizona, USA), Discovery life sciences (Alabama, USA), Folio Biosciences (by now merged to discovery life sciences). All materials were collected from donors for whom a written informed consent for a brain autopsy and the use of the material and clinical;sources (Additional file 1: Tables S2, S3, S4), including Tissue Solutions Ltd (Glasgow, UK), Analytical Biological Services Inc. (Delaware, USA), Banner sun health research institute (Arizona, USA), Discovery life sciences (Alabama, USA), Folio Biosciences (by now merged to discovery life sciences). All materials were collected from donors for whom a written informed consent for a brain autopsy aFull Paper
2023Stark, GF;Martin, RM;Smith, LE;Wei, B;Hellweger, FL;Bullerjahn, GS;McKay, RML;Boyer, GL;Wilhelm, SW;Microcystin aids in cold temperature acclimation: Differences between a toxic Microcystis wildtype and non-toxic mutantHarmful algae37951605RNA samples were sent to Discovery Life Sciences (previously Hudson Alpha, Huntsville, AL) where sample library prep and Illumina Novaseq 6000 platform sequencing (50 million reads, 150 bp, paired end) were performed after ribosomal RNA reduction. A TruSeq rRNA reduction was performed on all samples ex-silico, according to manufFull Paper
2023Yang, L;Zhang, T;Wang, P;Chen, W;Liu, W;He, X;Zhang, Y;Jin, S;Luo, Z;Zhang, Z;Wang, X;Liu, J;Imatinib and M351-0056 enhance the function of VISTA and ameliorate the development of SLE via IFN-I and noncanonical NF-?B pathwayCell biology and toxicology37804401The viability of Jurkat (Clone E6-1) cells or human peripheral blood mononuclear cells (PBMCs) (AllCells) cultured with compound imatinib was evaluated with CCK-8 ?it ( SaveFull Paper
2023Wang, M;Moore, A;Grego, A;Cowart, M;Rager, J;Paris, G;Ito, K;Babbar, N;Geeganage, S;Scherle, P;Bhagwat, N;Abstract C013: Clinical biomarkers based on PK/PD modeling to guide the development for a first-in-class, highly selective SMARCA2 (BRM) degrader, PRT3789Molecular Cancer TherapeuticsBackground ? The SWI/SNF family of chromatin-remodeling complexes is frequently dysregulated in multiple tumor types, resulting in aberrant expression of genes. SaveFull Paper
2023Osborne, MJ;Sulekha, A;Culjkovic-Kraljacic, B;Gasiorek, J;Ruediger, E;Jolicouer, E;Marinier, A;Assouline, S;Borden, KLB;Medicinal Chemistry and NMR Driven Discovery of Novel UDP-glucuronosyltransferase 1A Inhibitors That Overcome Therapeutic Resistance in CellsJournal of molecular biology38043731Corning Human supersomes TM UGT1A1 (456411) and UGT1A4 (456414) were procured from Discovery Life Sciences. The extent of specific substrate glucuronide SaveFull Paper
2023Ung, J;Tan, SF;Fox, TE;Shaw, JJP;Taori, M;Horton, BJ;Golla, U;Sharma, A;Szulc, ZM;Wang, HG;Chalfant, CE;Cabot, MC;Claxton, DF;Loughran, TP;Feith, DJ;Acid Ceramidase Inhibitor LCL-805 Antagonizes Akt Signaling and Promotes Iron-Dependent Cell Death in Acute Myeloid LeukemiaCancers38136410irginia School of Medicine and in accordance with the Declaration of Helsinki (Supplemental Table S1). Normal peripheral blood mononuclear cells (PBMCs) and bone marrow (BM) cells were purchased from AllCells (Alameda, CA, USA), Virginia Blood Services, Zen-Bio (Durham, NC, USA), and Inova Blood Donor Services (Sterling, VA, USA). PBMCs and BM mononuclear cells were isolated from patients’ wholeFull Paper
2023Sheinin, M;Mondal, S;Pahan, K;Neutralization of p40 Homodimer and p40 Monomer Leads to Tumor Regression in Patient-Derived Xenograft Mice with Pancreatic CancerCancers38136341matched healthy controls were purchased from Discovery Life Sciences, Los Osos, CA, Serum samples were purchased mainly from Discovery Life Sciences (DLS). Some Save Cached;om Rush University Medical Center. Controls are defined as healthy individuals without significant medical history. Details are included below (see Table 2). Serum samples were purchased mainly from Discovery Life Sciences (DLS). Some normal samples were obtained from Rush University Medical Center (RUMC). Each sample was analyzed for p40, p402, and IL-12 three times by ELISA. 2.3. Sandwich ELISFull Paper
2023Moreno, CS;Winham, CL;Alemozaffar, M;Klein, ER;Lawal, IO;Abiodun-Ojo, OA;Patil, D;Barwick, BG;Huang, Y;Schuster, DM;Sanda, MG;Osunkoya, AO;Integrated Genomic Analysis of Primary Prostate Tumor Foci and Corresponding Lymph Node Metastases Identifies Mutations and Pathways Associated with MetastasisCancers38067373) analysis was also performed by the EIGC on all RNA and DNA extractions and RNAseq and WES were performed by HudsonAlpha (subunit of Discovery Life Sciences). Save Cached;using the Covaris truXTRAC FFPE total NA kit. Quality control (QC) analysis was also performed by the EIGC on all RNA and DNA extractions and RNAseq and WES were performed by HudsonAlpha (subunit of Discovery Life Sciences). For RNAseq, we performed 100 bp paired-end sequencing using the SMART-Seq Stranded library prep kit (Takara, 634444). For WES analysis, libraries were prepared using the KAPAFull Paper
2023Melton, C;Freese, P;Zhou, Y;Shenoy, A;Bagaria, S;Chang, C;Kuo, C;Scott, E;Srinivasan, S;Cann, G;Roychowdhury-Saha, M;Chang, P;Singh, A;A Novel Tissue-Free Method to Estimate Tumor-Derived Cell-Free DNA Quantity Using Tumor Methylation PatternsCancersAdditional tissue and cell type samples had been obtained commercially via Discovery Life Sciences, Huntsville, AL, USA (formerly Conversant Biologics, Inc.). Tissue Save Cached;- Simple Summary Tumor cells shed pieces of DNA that circulate in biofluids like blood and urine. The amount of circulating tumor DNA is related to the amount of cancer in Save CachedFull Paper
2023Rice, M;Nuovo, GJ;Sawant, D;Mishra, A;Tili, E;Comparison of Neuroinflammation Induced by Hyperphosphorylated Tau Protein Versus Ab42 in Alzheimer's DiseaseMolecular neurobiology38105410The patient samples (formalin-fixed paraffin-embedded autopsy material) originated from the files of Folio Biosciences. Clinical information included the diagnosis, age/sex SaveFull Paper
2023Jasani, B;Taniere, P;Schildhaus, HU;Blighe, K;Parry, S;Wilkinson, D;Atkey, N;Clare-Antony, S;McCabe, C;Quinn, C;CLDN Study Group, ;Dodson, A;Global Ring Study to Investigate the Comparability of Total Assay Performance of Commercial Claudin 18 Antibodies for Evaluation in Gastric CancerLaboratory investigation; a journal of technical methods and pathology37949357Discovery Life Sciences (DLS) Biomarker Services GmbH, Kassel, Germany.++University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK.++Discovery Life Sciences (DLS) Biomarker Services GmbH, Kassel, Germany.++Clinical Bioinformatics Research Ltd, London, UK.++UK National External Quality Assessment Scheme for Immunocytochemistry & In-Situ Hybridisation, London, UK.++UK National External Quality Assessment Scheme for Immunocytochemistry & In-Situ Hybridisation, London, UK.++Diaceutics PLC, Belfast, UK.++Diaceutics PLC, Belfast, UK.++Diaceutics PLC, Belfast, UK.++Diaceutics PLC, Belfast, UK. Electronic address: christine.quinn@diaceutics.com.++See the details of CLDN Study Group members in the acknowledgments.++UK National External Quality Assessment Scheme for Immunocytochemistry & In-Situ Hybridisation, London, UKFull Paper
2024Zhang, D;Zhou, G;Thongda, W;Li, C;Ye, Z;Zhao, H;Beck, BH;Mohammed, H;Peatman, E;Early divergent responses to virulent and attenuated vaccine isolates of Flavobacterium covae sp. nov. In channel catfish, Ictalurus punctatusFish & shellfish immunology38030028The RNA-Seq library was constructed and sequenced by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were constructed with 2.14-3.25 ?g of SaveFull Paper
2023Mairinger, E;Wessolly, M;Buderath, P;Borchert, S;Henrich, L;Mach, P;Steinborn, J;Kimming, R;Jasani, B;Schmid, KW;Bankfalvi, A;Mairinger, FD;Tumor cell cytoplasmic metallothionein expression associates with differential tumor immunogenicity and prognostic outcome in high-grade serous ovarian carcinomaFrontiers in oncology38023247Institute of Pathology, University Hospital Essen, Essen, Germany.++Department of Gynecology and Obstetrics, University Hospital Essen, Essen, Germany.++Department of Gynecology and Obstetrics, University Hospital Essen, Essen, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany.++Department of Gynecology and Obstetrics, University Hospital Essen, Essen, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany.++Department of Gynecology and Obstetrics, University Hospital Essen, Essen, Germany.++Department of Pathology, Targos - A Discovery Life Sciences Company, Kassel, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany.++Institute of Pathology, University Hospital Essen, Essen, Germany.++Institute of Pathology, University Hospital Essen, Essen, GermanyFull Paper
2023Bonnevie, ED;Dobrzynski, E;Steiner, D;Hildebrand, D;Monslow, J;Singh, M;Decman, V;Krull, DL;A machine learning approach toward automating spatial identification of LAG3+/CD3+ cells in ulcerative colitisScientific reports38066073Human biological samples were sourced ethically from commercial vendors (BioIVT/Asterand and Discovery Life Sciences) that have been approved by GSK human Save;e to better understand disease progression and treatment potential. Materials and methods Method overview Human biological samples were sourced ethically from commercial vendors (BioIVT/Asterand and Discovery Life Sciences) that have been approved by GSK human biological sample management ethical committee. Prior to sample acquisition informed consent for general research was obtained by commerciFull Paper
2023Gao, L;Kyubwa, EM;Starbird, MA;Diaz de Leon, J;Nguyen, M;Rogers, CJ;Menon, N;Circulating miRNA profiles in COVID-19 patients and meta-analysis: implications for disease progression and prognosisScientific reports38065980effectiveness. Materials and methods Clinical specimen Adult patients hospitalized in Erie County, NY, with PCR-confirmed COVID-19 between March and November 2020 were retrospectively identified by Discovery Life Sciences (DLS; Huntsville, AL). Plasma samples were collected within the initial seven days of hospitalization. Demographic and clinical profiles, encompassing laboratory data, were extFull Paper
2023Stecklein, SR;Barlow, W;Pusztai, L;Timms, K;Kennedy, R;Logan, GE;Seitz, R;Badve, S;Gökmen-Polar, Y;Porter, P;Linden, H;Tripathy, D;Hortobagyi, GN;Godwin, AK;Thompson, A;Hayes, DF;Sharma, P;Dual Prognostic Classification of Triple-Negative Breast Cancer by DNA Damage Immune Response and Homologous Recombination DeficiencyJCO precision oncology37972336peakers’ Bureau: Genomic Health, Targos GmbH, Agilent Research Funding: Dako/Agilent Technologies (Inst), Lilly (Inst), Bristol Myers Squibb Foundation (Inst) Patents, Royalties, Other Intellectual Property: EarlyR—signature for ER1 breast cancer (Inst), E-Score for predicting recuFull Paper
2023Robertson, IB;Mulvaney, R;Dieckmann, N;Vantellini, A;Canestraro, M;Amicarella, F;O'Dwyer, R;Cole, DK;Harper, S;Dushek, O;Kirk, P;Tuning the potency and selectivity of ImmTAC molecules by affinity modulationClinical and experimental immunology37930865T cell engaging bispecifics have great clinical potential for the treatment of cancer and infectious diseases. The binding affinity and kinetics of a bispecific molecule for both SaveFull Paper
2023Scheel, AH;Lamberty, H;Tolkach, Y;Gebauer, F;Schoemig-Markiefka, B;Zander, T;Buettner, R;Rueschoff, J;Bruns, CJ;Schroeder, W;Quaas, A;Tumour area infiltration and cell count in endoscopic biopsies of therapy-naive upper GI tract carcinomas by QuPath analysis: implications for predictive biomarker testingScientific reports37845307Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.++Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.++Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.++Department of General, Visceral and Cancer Surgery, University Hospital Cologne, Medical Faculty, University of Cologne, Cologne, Germany.++Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.++Department of Internal Medicine I, University Hospital Cologne, Medical Faculty, University of Cologne, Cologne, Germany.++Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany.++Targos Molecular Pathology GmbH, Kassel, Germany.++Department of General, Visceral and Cancer Surgery, University Hospital Cologne, Medical Faculty, University of Cologne, Cologne, Germany.++Department of General, Visceral and Cancer Surgery, University Hospital Cologne, Medical Faculty, University of Cologne, Cologne, Germany.++Institute of Pathology, University Hospital Cologne, Medical Faculty, University of Cologne, Kerpener Str. 62, 50937, Cologne, Germany. alexander.quaas@uk-koeln.deFull Paper
2023Van den Ackerveken, P;Lobbens, A;Pamart, D;Kotronoulas, A;Rommelaere, G;Eccleston, M;Herzog, M;Epigenetic profiles of elevated cell free circulating H3.1 nucleosomes as potential biomarkers for non-Hodgkin lymphomaScientific reports37770512by Discovery Life Sciences (Discovery Life Sciences, Huntsville, Texas, USA; protocol/study). For healthy donors (iNoSpecimens BioBank, Clermont Ferrand, France), the SaveFull Paper
2023Pan, Y;Rohde, M;Zeitler, J;Namburi, SVS;Cao, L;Hu, J;Meyer, K;Lu, Y;A sensitive AAV transduction inhibition assay assists evaluation of critical factors for detection and concordance of pre-existing antibodiesMolecular therapy. Methods & clinical development37920239g PBS and 0.001% Pluronic F-68. AAV copy concentration were determined by qPCR. Human serum samples Individual human serum samples from healthy donors were purchased from BioIVT (Westbury, NY, USA), Discovery Life Sciences (Los Osos, CA, USA), and Golden West Biologicals (Temecula, CA, USA). Donors varied in ethnicity (Black, White, Hispanic, and Asian), ages, and geographical locations in the UnFull Paper
2023Poletti, V;Montepeloso, A;Pellin, D;Biffi, A;Prostaglandin E2 as transduction enhancer affects competitive engraftment of human hematopoietic stem and progenitor cellsMolecular therapy. Methods & clinical development37920236of a modification of our current transduction protocol with the addition of PGE2. Materials and methods Human cell culture and transduction Human CD34+ cells from healthy donors were purchased from AllCells. After thawing, cells were pre-activated for 24 h in GMP SCGM medium (CellGenix) added of a human recombinant cytokines cocktail (PeproTech, Inc.), as previously published.17 Three hundred tFull Paper
2023Lazarski, CA;Hanley, PJ;Review of flow cytometry as a tool for cell and gene therapyCytotherapy37943204PJH is a co-founder and on the board of directors of Mana Therapeutics. He is or was an advisor or on the scientific advisory board of: Cellenkos, Cellevolve, Discovery Life Sciences, Capsida, Autolomous, MicrofluidX and March Biosciences. CAL has no disclosuresFull Paper
2023Moore, JL;Charkoftaki, G;A Guide to MALDI Imaging Mass Spectrometry for TissuesJournal of proteome research37877579Department of Proteomics, Discovery Life Sciences, Huntsville, Alabama 35806, United States.++Department of Environmental Health Sciences, Yale School of Public Health, Yale University, New Haven, Connecticut 06520, United StatesFull Paper
2024O'Brien, G;Cecotka, A;Manola, KN;Pagoni, MN;Polanska, J;Badie, C;Epigenetic signature of ionizing radiation in therapy-related AML patientsHeliyon38163095Research (NICR), Newcastle REC (17/NE/0361). Bone marrow aspirate samples were also obtained from 5 healthy donors from AllCells® (Alamada, USA)Full Paper
2023Hemken, PM;Qin, X;Sokoll, LJ;Jackson, L;Feng, F;Li, P;Gawel, SH;Tu, B;Lin, Z;Hartnett, J;Hawksworth, D;Tieman, BC;Yoshimura, T;Kinukawa, H;Ning, S;Liu, E;Meng, F;Chen, F;Miao, J;Mi, X;Tong, X;Chan, DW;Davis, GJ;Validation of the novel GLAS algorithm as an aid in the detection of liver fibrosis and cirrhosis based on GP73, LG2m, age, and sexClinical proteomics38017436iver disease. An additional set of serum samples (collectively referred to as the Western Vendor Cohort, WVC; n?=?246) were purchased from BioIVT (Wesbury, NY), Biomex GmbH (Heidelberg, Germany), Discovery Life Sciences (Huntsville, AL), and ProMedDx (Norton, MA). These three sample sets (JHU/UTSMC/WVC) were combined to develop and train the liver fibrosis and cirrhosis diagnostic algorithm (dFull Paper
2023Chen, S;Li, X;Li, Y;He, X;Bryant, M;Qin, X;Li, F;Seo, JE;Guo, X;Mei, N;Guo, L;The involvement of hepatic cytochrome P450s in the cytotoxicity of lapatinibToxicological sciences : an official journal of the Society of Toxicology37788138were purchased from Discovery Life Sciences (Columbia (UCRM-A, Discovery Life Sciences) and seeded in 384- -A (UPCM-A, Discovery Life Sciences) for 4 h prior to the SaveFull Paper
2023LeCleir, GR;Bassett, J;Wilhelm, SW;Effects of iron concentration and DFB (Desferrioxamine-B) on transcriptional profiles of an ecologically relevant marine bacteriumPloS one38100448les were subjected to a subsequent round of DNAse treatment. All samples were verified to be free from genomic DNA within two rounds of DNAse treatment. The Hudson Alpha Genome Sequencing Center (now Discovery Life Sciences; https://www.hudsonalpha.org/gsc/) performed rRNA reduction and mRNA transcript library sequencing using a 2 x 100 nucleotide protocol on the Illumina Novaseq platform. NucleotFull Paper
2023Shyu, JY;Schlag, PA;Karwowska, SM;Manohar, CF;Truong, HM;Longshore, JW;Zhang, G;Performance of the cobas EZH2 mutation test on clinical samples from non-Hodgkin lymphoma patientsPloS one38096164Objective To present the technical verification and clinical validation of the companion diagnostic assay, cobas EZH2 Mutation Test (cobas EZH2 Test), targeting gain-of- Save Cached;ous mutant variants of EZH2 were acquired from 14 vendors (Analytical Biological Services, Inc.; Asterand, Inc.; Aurora Diagnostics, Inc.; Bio-Options, Inc.; BioServe, Inc.; Cambridge Biosource, LLC; Conversant Bio; Folio Biosciences; ILSbio, LLC; Indivumed GmbH; MT Group; PhenoPath, PLLC; ProteoGenex, Inc.; Tissue Source, LLC). These were de-identified remnant FFPE tumor tissue samples from biopsFull Paper
2023Karbhari, A;Mosessian, S;Trivedi, KH;Valla, F;Jacobson, M;Truty, MJ;Patnam, NG;Simeone, DM;Zan, E;Brennan, T;Chen, H;Kuo, PH;Herrmann, K;Goenka, AH;Gallium-68-labeled fibroblast activation protein inhibitor-46 PET in patients with resectable or borderline resectable pancreatic ductal adenocarcinoma: A phase 2, multicenter, single arm, open label non-randomized study protocolPloS one38011131sections from biopsied or resected specimens will be evaluated by standard histopathologic methods at an independent centralized core facility (Discovery Life Sciences, Save Cached;Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of America.++Clinical Development, Sofie Biosciences, Dulles, Virginia, United States of America.++Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of America.++Radiopharmaceutical and Contract Manufacturing, Sofie Biosciences, Dulles, Virginia, United States of America.++Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of America.++Department of Surgery, Mayo Clinic, Rochester, Minnesota, United States of America.++Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of America.++Departments of Surgery and Pathology, NYU Langone Health, New York, New York, United States of America.++Department of Radiology, Weill Cornell Medicine, New York, New York, United States of America.++Discovery Life Sciences, Newtown, Pennsylvania, United States of America.++Discovery Life Sciences, Newtown, Pennsylvania, United States of America.++Departments of Medical Imaging, Medicine and Biomedical Engineering, University of Arizona, Tucson, Arizona, United States of America.++Department of Nuclear Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany.++Department of Radiology, Mayo Clinic, Rochester, Minnesota, United States of AmericaFull Paper
2023Zahavi, DJ;Erbe, R;Zhang, YW;Guo, T;Malchiodi, ZX;Maynard, R;Lekan, A;Gallagher, R;Wulfkuhle, J;Petricoin, E;Jablonski, SA;Fertig, EJ;Weiner, LM;Antibody dependent cell-mediated cytotoxicity selection pressure induces diverse mechanisms of resistanceCancer biology & therapy37878417Fresh healthy donor NK cells from five different donors were purchased from AllCells with CD56 negative selection (PB012-N). Donor NK cells were expanded and SaveFull Paper
2023Mohebbi Najm Abad, J;Farahbakhsh, A;Mir, M;Alizadeh, R;Hekmatmanesh, A;Urea-Self Powered Biosensors: A Predictive Evolutionary Model for Human Energy HarvestingSensors (Basel, Switzerland)37837010modified Pt electrode Turk. J. Chem. 2019 43 1063 1074 10.3906/kim-1901-13 21. Ramezani AzghandiO. FarahbakhshA. The obtain optimum production conditions for Glucose Oxidase biosensor using software Qualtek-4 Int. J. Nano Dimens. (IJND) 2015 6 23 30 10.7508/IJND.2015.06.004 22. YangW. HanW. GaoH. ZhangL. WangS. XingL. ZhangY. XueX. Self-powered implantable electronic-skin for in situ analysis ofFull Paper
2023Baze, A;Wiss, L;Horbal, L;Biemel, K;Asselin, L;Richert, L;Comparison of in vitro thyroxine (T4) metabolism between Wistar rat and human hepatocyte culturesToxicology in vitro : an international journal published in association with BIBRA38142784ts and media used are listed as follows. Universal Cryopreservation Recovery Medium (UCRM™) and Universal Primary Cells Plating Medium (UPCM™) were purchased from Discovery Life Sciences (DLS, Columbia, MD). INVITROGRO CP Rodent Medium and Torpedo Rodent Antibiotic Mix were purchased from BioIVT (Baltimore, Maryland, US), Matrigel® and Collagen I Rat Tail from Corning (Avon, France) and William's medium E (WE)?+?GLUTAMAX, Insulin-Transferrin-Selenium (ITS), phosphate-buffered saline (PBS) 1×, Dulbecco's Modified Eagle Medium (DMEM), MagMAX mirVana Total RNA Isolation Kit and Penicillin/Full Paper
2023Ali, KA;Shah, RD;Dhar, A;Myers, NM;Nguyen, C;Paul, A;Mancuso, JE;Scott Patterson, A;Brody, JP;Heiser, D;Ex vivo discovery of synergistic drug combinations for hematologic malignanciesSLAS discovery : advancing life sciences R & D38101570Bone marrow samples from healthy volunteers were supplied by AllCells, Inc. (Alameda, CA). This study was reviewed by the WCG Institutional Review Board and SaveFull Paper
2023Ferri, N;De Martin, S;Stuart, J;Traversa, S;Folli, F;Pappagallo, M;O'Gorman, C;Guidetti, C;Mattarei, A;Inturrisi, CE;Manfredi, PL;Drug-Drug Interaction Studies of Esmethadone (REL-1017) Involving CYP3A4- and CYP2D6-Mediated MetabolismDrugs in R&D38010591Background and Objective Esmethadone (dextromethadone; d-methadone; S-methadone (+)-methadone; REL-1017) is the opioid inactive dextro-isomer of racemic Save;- Thawing medium (Universal Cryo Recovery Medium) for hepatocytes was purchased from In Vitro ADMET Laboratories (iVAL, Columbia, MD, USA). Hepatocyte culture SaveFull Paper
2023Vertacnik, KL;Herrig, DK;Godfrey, RK;Hill, T;Geib, SM;Unckless, RL;Nelson, DR;Linnen, CR;Evolution of five environmentally responsive gene families in a pine-feeding sawfly, Neodiprion lecontei (Hymenoptera: Diprionidae)Ecology and evolution37791292sessed with 0.7% agarose gel, purity was measured with the 260/280 ratio, and concentration was measured with a Quant?iT dsDNA High?Sensitivity fluorescence assay (Thermo Fisher Scientific). The HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) prepared and sequenced the DNA libraries. Two small?insert, barcoded libraries with average fragment sizes of 337?bp and 864?bp were made fFull Paper
2023Chalmers, SB;van der Wal, T;Fre, S;Jonkers, J;Fourteenth Annual ENBDC Workshop: Methods in Mammary Gland Biology and Breast CancerJournal of mammary gland biology and neoplasia37801168Sandra Schoniger (Discovery Life Sciences Biomarker Services GmbH, Kassel, Germany) gave the final presentation of the session, focussed on the characterisation of Save;l hotspots of Ki67 colocalised with immune cell aggregates that resembled tertiary lymphoid structures, a hitherto unrecognised component of the ovine mammary gland immune system. Sandra Schöniger (Discovery Life Sciences Biomarker Services GmbH, Kassel, Germany) gave the final presentation of the session, focussed on the characterisation of mammary tumours in pet rabbits. Rabbits may representFull Paper
2023Koc, MA;Wiles, TA;Weinhold, DC;Rightmyer, S;Weaver, AL;McDowell, CT;Roder, J;Asmellash, S;Pestano, GA;Roder, H;Georgantas Iii, RW;Molecular and translational biology of the blood-based VeriStrat® proteomic test used in cancer immunotherapy treatment guidanceJournal of mass spectrometry and advances in the clinical lab38074293LC immunotherapy. 2 Material and methods 2.1 Samples De-identified, serum samples from human subjects diagnosed with lung cancer were acquired from commercial biobanks under their consent protocols [Discovery Life Sciences (Huntsville, AL), ProMedDx (Norton, MA), ProteoGenex (Inglewood, CA) and BioIVT (Westbury, NY)]. Additional archived, de-identified samples were from patients consented into thFull Paper
2023Okada, K;Yokota, J;Yamashita, T;Inui, T;Kishimoto, W;Nakase, H;Mizuguchi, H;Establishment of human intestinal organoids derived from commercially available cryopreserved intestinal epithelium and evaluation for pharmacokinetic studyDrug metabolism and pharmacokinetics38064926Following the manufacturer's instructions, cryopreserved human intestinal epithelial cells (Cryopreserved Human Enterocytes, Discovery Life Science, AL, USA) were thawed by incubation in a 37 °C water bath for 90 s. The cells were then centrifuged at 100 g for 10 min to create a pellet. The supernatant was removed and suspended in Matrigel. The pellet was resuspended in Matrigel, and a 40 ?L aliquot of the resulting cell suspension was added to the center of each well of a 24-well plate. The Matrigel was polymerized for 10Full Paper
2023Shaikh, N;Zhang, X;Abdalla, M;Baylink, D;Tang, X;Enhancing Human Treg Cell Induction through Engineered Dendritic Cells and Zinc SupplementationCritical Reviews in ImmunologyThese 102 PBMCs were isolated from a Leukopak obtained from AllCells, LLC. The study was reviewed by 103 the Long Island University Institutional Review Board (IRB) SaveFull Paper
2023Rüschoff, J;Schildhaus, HU;Rüschoff, JH;Jöhrens, K;Bocker-Edmonston, T;Dietmaier, W;Bläker, H;Baretton, G;Horst, D;Dietel, M;Hartmann, A;Klauschen, F;Merkelbach-Bruse, S;Stenzinger, A;Schöniger, S;Tiemann, M;Weichert, W;Büttner, R;[Not Available]Pathologie (Heidelberg, Germany)37656216Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland. josef.rueschoff@dls.com.++Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland.++Institut für Pathologie und Molekularpathologie, Universitätsspital Zürich, Zürich, Schweiz.++Institut für Pathologie, Universitätsklinikum Carl Gustav Carus Dresden, Dresden, Deutschland.++Department of Pathology, Cooper University Health Care, Camden, NJ, USA.++Institut für Pathologie/Zentrum für molekularpathologische Diagnostik, Universität Regensburg, Regensburg, Deutschland.++Institut für Pathologie, Universitätsklinikum Leipzig, Leipzig, Deutschland.++Institut für Pathologie, Universitätsklinikum Carl Gustav Carus Dresden, Dresden, Deutschland.++Institut für Pathologie, Charité - Universitätsmedizin Berlin, Campus Mitte, Berlin, Deutschland.++Institut für Pathologie, Charité - Universitätsmedizin Berlin, Campus Mitte, Berlin, Deutschland.++Pathologisches Institut, Universität Erlangen-Nürnberg, Erlangen, Deutschland.++Pathologisches Institut, Ludwig-Maximilians-Universität München, München, Deutschland.++Institut für Pathologie, Universitätsklinikum Köln, Köln, Deutschland.++Pathologisches Institut, Universitätsklinikum Heidelberg, Heidelberg, Deutschland.++Discovery Life Sciences Biomarker GmbH und Pathologie Nordhessen, Germaniastr. 7, 34119, Kassel, Deutschland.++Institut für Hämatopathologie Hamburg, Hamburg, Deutschland.++Institut für Pathologie, Technische Universität München, München, Deutschland.++Institut für Pathologie, Universitätsklinikum Köln, Köln, DeutschlandFull Paper
2023Abidin, MR;Alpan, O;Plassmeyer, M;Kozhaya, L;Loizou, D;Dogan, M;Upchurch, Z;Manes, NP;Nita-Lazar, A;Unutmaz, D;Sønder, SU;STAT4 Phosphorylation of T-helper Cells predicts surgical outcomes in Refractory Chronic RhinosinusitismedRxiv : the preprint server for health sciences38168423Healthy adult blood was obtained from AllCells. Primary CD4 T cells were isolated, activated, 183 transduced to overexpress IL12Rb1 wild-type or mutant, and proliferated SaveFull Paper
2023Landberg, N;Köhnke, T;Feng, Y;Nakauchi, Y;Fan, AC;Linde, MH;Karigane, D;Lim, K;Sinha, R;Malcovati, L;Thomas, D;Majeti, R;IDH1-mutant preleukemic hematopoietic stem cells can be eliminated by inhibition of oxidative phosphorylationBlood cancer discovery38091010Healthy adult peripheral blood-derived CD34+ 458 HSPCs were purchased from AllCells. Mononuclear cells from AML patients were isolated 459 from peripheral blood or SaveFull Paper
2023Rumker, L;Sakaue, S;Reshef, Y;Kang, JB;Yazar, S;Alquicira-Hernandez, J;Valencia, C;Lagattuta, KA;Mah-Som, A;Nathan, A;Powell, JE;Loh, PR;Raychaudhuri, S;Identifying genetic variants that influence the abundance of cell states in single-cell databioRxiv : the preprint server for biology38014313y the resulting simulated SNP decays. We generated 94 × 10 × 100 = 94,000 total simulates. We ran GeNA to conduct one csaQTL survey for associations per single-cell data object (T, B, NK, myeloid, allcells) using as input all SNPs defined to associate with true phenotypes within that data object. We ran GeNA with default parameters and controlled for age, sex, batch and gPC1-6 to generate anFull Paper
2023Filosto, S;Vardhanabhuti, S;Canales, MA;Poire, X;Lekakis, LJ;de Vos, S;Portell, CA;Wang, Z;To, C;Schupp, M;Poddar, S;Trinh, T;Warren, CM;Aguilar, EG;Budka, J;Cheng, P;Chou, J;Bot, A;Shen, RR;Westin, JR;Product attributes of CAR T-cell therapy differentially associate with efficacy and toxicity in second-line large B-cell lymphoma (ZUMA-7)Blood cancer discovery37983485CAR T-cell products were generated from apheresis material from healthy 478 donors (AllCells) following the axi-cel manufacturing protocol. Anti-CD19 CAR T effector SaveFull Paper
2023Ho, C;Gopal, AK;Till, BG;Shadman, M;Lynch, RC;Cowan, AJ;Wu, QV;Voutsinas, J;Rasmussen, HA;Blue, K;Ujjani, CS;Cassaday, RD;Fromm, JR;Fang, M;Smith, SD;Pembrolizumab With R-CHOP in Previously Untreated DLBCL: Sustained, High Efficacy, and Safety With Long-Term Follow-UpClinical lymphoma, myeloma & leukemia37926672PD-L1 expression was evaluated as an exploratory endpoint, and PD-L1 immunohistochemistry (IHC) on available tissue samples was performed by QualTek Molecular SaveFull Paper
2023Tierney, BT;Kim, J;Overbey, EG;Ryon, KA;Foox, J;Sierra, M;Bhattacharya, C;Damle, N;Najjar, D;Park, J;Garcia Medina, S;Houerbi, N;Meydan, C;Wain Hershberg, J;Qiu, J;Kleinman, A;Al Ghalith, G;MacKay, M;Afshin, EE;Dhir, R;Borg, J;Gatt, C;Brereton, N;Readhead, B;Beyaz, S;Venkateswaran, KJ;Blease, K;Moreno, J;Boddicker, A;Zhao, J;Lajoie, B;Scott, RT;Altomare, A;Kruglyak, S;Levy, S;Church, G;Mason, CE;Viral activation and ecological restructuring characterize a microbiome axis of spaceflight-associated immune activationResearch square37886447For metatranscriptomic sequencing, library preparation and sequencing were performed at 746 Discovery Life Sciences (Huntsville, Alabama). The extracted RNA went Save;sequenced on a S4 flow cell of the Illumina NovaSeq 6000 Sequencing System with 2 × 150 bp paired-end reads. For metatranscriptomic sequencing, library preparation and sequencing were performed at Discovery Life Sciences (Huntsville, Alabama). The extracted RNA went through an initial purification and cleanup with DNase digestion using the Zymo Research RNA Clean & Concentrator Magbead Kit (catFull Paper
2023Frigault, MM;Mithal, A;Wong, H;Stelte-Ludwig, B;Mandava, V;Huang, X;Birkett, J;Johnson, AJ;Izumi, R;Hamdy, A;Enitociclib, a Selective CDK9 Inhibitor, Induces Complete Regression of MYC+ Lymphoma by Downregulation of RNA Polymerase II Mediated TranscriptionCancer research communications37882668Differential gene expression analysis was performed (Fios Genomics) from RNA-seq data (Discovery Life Sciences). MYC and MCL1 mRNA levels were measured by Save;ed total RNA was evaluated using an Agilent 2100 Bioanalyzer system and RNA 6000 Nano Kit (Agilent, #5067-1511). Differential gene expression analysis was performed (Fios Genomics) from RNA-seq data (Discovery Life Sciences). MYC and MCL1 mRNA levels were measured by qPCR in vitro and in vivo, using a Taqman qPCR system (Thermo Fisher Scientific), TaqMan Fast Universal PCR Master Mix (2X) no AmpErFull Paper
2023Jin, L;Wang, F;Wang, X;Harvey, BP;Bi, Y;Hu, C;Cui, B;Darcy, AT;Maull, JW;Phillips, BR;Kim, Y;Jenkins, GJ;Sornasse, TR;Tian, Y;Identification of Plasma Biomarkers from Rheumatoid Arthritis Patients Using an Optimized Sequential Window Acquisition of All THeoretical Mass Spectra (SWATH) Proteomics WorkflowProteomes37873874with methotrexate for at least three months. Only baseline plasma samples (K2-EDTA blood collection) were selected for this experiment. EDTA plasma samples from healthy volunteers were obtained from Conversant Biologics, Inc., Huntsville, Alabama, USA. 2.2. Sample Fractionation and Spectral Library Generation 2.2.1. Sample Preparation To prepare the experiment-specific plasma peptide spectrum li ... with methotrexate for at least three months. Only baseline plasma samples (K2-EDTA blood collection) were selected for this experiment. EDTA plasma samples from healthy volunteers were obtained from Conversant Biologics, Inc., Huntsville, Alabama, USA. 2.2. Sample Fractionation and Spectral Library Generation 2.2.1. Sample Preparation To prepare the experiment-specific plasma peptide spectrum liFull Paper
2023Hinestrosa, JP;Sears, RC;Dhani, H;Lewis, JM;Schroeder, G;Balcer, HI;Keith, D;Sheppard, BC;Kurzrock, R;Billings, PR;Development of a blood-based extracellular vesicle classifier for detection of early-stage pancreatic ductal adenocarcinomaCommunications medicine37857666against this deadly disease. Methods Specimen collection The specimens for the training set were obtained from commercial biorepositories (ProteoGenex, Culver City, CA; DxBiosamples, San Diego, CA; Discovery Life Sciences, Huntsville, AL; Fox Chase Cancer Center, Philadelphia, PA; iSpecimen, Lexington MA; Tissue for Research, Newmarket, England). The specimens for the validation set were obtaineFull Paper
2023Adema, V;Ganan-Gomez, I;Ma, F;Rodriguez-Sevilla, JJ;Chien, K;Yang, H;Thongon, N;Kanagal-Shamanna, R;Loghavi, S;Montalban-Bravo, G;Hammond, D;Gu, Y;Tan, R;Tan, L;Lorenzi, P;Al-Atrash, G;Clise-Dwyer, K;Bejar, R;Pellegrini, M;Garcia-Manero, G;Colla, S;IL-1?-mediated inflammatory signaling drives ineffective erythropoiesis in early-stage myelodysplastic syndromesbioRxiv : the preprint server for biology37808770Myelodysplastic syndromes (MDS) are a group of incurable hematopoietic stem cell (HSC) neoplasms characterized by peripheral blood cytopenias and a high risk of SaveFull Paper
2023Prakash, S;Bharti, S;Priyatma, AR;Performance Characteristic of a Simple, Rapid and Cost-Effective Micro-PCR Assay for HBV DNA QuantificationJ Med Diagn MethThe genotype panel was obtained from Discovery Life Sciences (DLS). After duplicate extraction of the corresponding genotype DNA using TruePrep Auto, PCR was SaveFull Paper
2023Gang, L;Li, H;Ge, P;Yu, J;Whether AI+CDK4/6i+HP is suitable treatment for three positive metastatic breast cancer patient with renal insufficiency caused by type 2 diabetesResearch SquareBackground: Hormone-receptor positive (HR+), ERBB2 gene mutation (HER2+) breast cancer is a very common type of breast cancer, but it is relatively rare for metastatic SaveFull Paper
2023Weiner, L;Fitzgerald, A;Maynard, R;Marcisak, E;Nasir, A;Glasgow, E;Jablonski, S;Veken, P;Pearson, G;Eisman, S;Mace, E;Fertig, E;Fibroblast activation protein regulates natural killer cell migration, extravasation and tumor infiltrationResearch SquareFresh healthy donor NK cells were purchased from AllCells with either CD56 positive selection or CD56 negative selection (Allcells, cat#PB012-P or PB012-N). For 2D SaveFull Paper
2023Chen, C;Hamilton, E;LoRusso, P;Rottey, S;Papadopoulos, K;Kummar, S;Kotecki, N;Thistlethwaite, F;LeBruchec, Y;Dyczkowski, J;Rüschoff, J;Samari, S;Toukam, M;Boni, J;Havenith, K;Pantano, S;Puzanov, I;608?Results of a phase 1 study investigating camidanlumab tesirine as monotherapy and in combination with pembrolizumab in patients with selected advanced solid tumorsRegular and Young Investigator Award AbstractsUSA * 7Institut Jules Bordet, Brussels, Belgium * 8The Christie NHS Foundation Trust and University of Manchester, Manchester, UK * 9ADC Therapeutics SA, Épalinges, Switzerland * 10Targos - A Discovery Life Sciences Company, Kassel, Germany * 11ADC Therapeutics (UK) Ltd, London, UK * 12ADC Therapeutics America, Inc, Murray Hill, NJ, USA * 13Roswell Park Comprehensive Cancer Center, BuffFull Paper
2023Melocchi, A;Schmittlein, B;Jones, A;Ainane, Y;Rizvi, A;Chan, D;Dickey, E;Pool, K;Harsono, K;Szymkiewicz, D;Scarfogliero, U;Bhatia, V;Sivanantham, A;Kreciglowa, N;Hunter, A;Gomez, M;Tanner, A;Uboldi, M;Batish, A;Balcerek, J;Kutova-Stoilova, M;Paruthiyil, S;Acevedo, L;Stadnitskiy, R;Carmichael, S;Aulbach, H;Hewitt, M;De Mollerat Du Jeu, X;Robilant, B;Parietti, F;Esensten, J;Development of a robotic cluster for automated and scalable cell therapy manufacturingbioRxivHuman CD8+ T cells isolated from commercially available human leukopheresis collections (StemExpress, US-CA or AllCells, US-CA) using Miltenyi CD8+ microbeads and a Miltenyi Prodigy instrument were used for run 1. Purified human CD8+ T cells were purchased from a commercial source IQ Biosciences and used for runs 2 and 3 (US-CA)Full Paper
2023Faccioli, L;Sun, Y;Motomura, T;Liu, Z;Kurihara, T;Hu, Z;Cetin, Z;Franks, J;Stolz, D;Ostrowska, A;Florentino, R;Fox, I;Soto-Gutierrez, A;Human Induced Pluripotent Stem Cell based Hepatic-Modeling of Lipid metabolism associated TM6SF2 E167K variantbioRxivBACKGROUND AND AIMS: TM6SF2 rs58542926 (E167K) is associated with an increase in the prevalence of Metabolic Disfunction-Associated Steatotic Liver Disease ( Save;- Cryopreserved primary hepatocytes from healthy individuals were obtained from In Vitro ADMET Laboratories Inc. (IVAL, Columbia, MD, USA). End-stage liver disease ( SaveFull Paper
2023Perez-Bermejo, J;Efagene, O;Matern, W;Holden, J;Kabir, S;Chew, G;Andreoletti, G;Catton, E;Ennis, C;Garcia, A;Gerstenberg, T;Hill, K;Jain, A;Krassovsky, K;Lalisan, C;Lord, D;Quejarro, B;Sales-Lee, J;Shah, M;Silva, B;Skowronski, J;Strukov, Y;Thomas, J;Veraz, M;Vijay, T;Wallace, K;Yuan, Y;Grogan, J;Wienert, B;Lahiri, P;Treusch, S;Dever, D;Soros, V;Partridge, J;Seim, K;Functional Screening in human HSPCs identifies optimized protein-based enhancers of Homology Directed RepairbioRxivCD34+ HSPCs were purchased from 544 AllCells and were isolated from G-CSF-mobilized peripheral blood from healthy donors. CD34+ HSPCs 545 were cultured at 2.5× SaveFull Paper
2023Chen, W;Yan, Y;Young, B;Pinto, A;Jiang, Q;Song, N;Yao, W;Zhang, D;Zhang, J;Microsatellite Instability Detection in Clinical Cancer Samples: A Multiplex qPCR Approach without Matching Normal SamplesmedRxiv48 samples with MSI status confirmed by PCR were purchased from 94 Discovery Life Sciences. All clinical samples information is collected from vendors and 95 SaveFull Paper
2023Cieniewicz, B;Oliveira, E;Saxton, M;Torabi, D;Bhatta, A;Kukutla, P;Arballo, A;Yang, Z;Yu, B;Fate, M;Ning, H;Corey, L;Maiti, A;Corey, D;Therapeutic Targeting of TIM-4-L With Engineered T Cells for Acute Myeloid LeukemiabioRxivPatient-derived AML bone marrow and PBMC samples and healthy donor controls were obtained from a contract research organization (Discovery Life Sciences). SaveFull Paper
2023Kocas-Kilicarslan, Z;Cetin, Z;Faccioli, L;Motomura, T;Amirneni, S;Diaz-Aragon, R;Florentino, R;Sun, Y;Pla-Palacin, I;Xia, M;Miedel, M;Kurihara, T;Hu, Z;Ostrowska, A;Wang, Z;Constantine, R;Li, A;Taylor, D;Behari, J;Soto-Gutierrez, A;Tafaleng, E;Polymorphisms Associated With Metabolic Dysfunction-Associated Steatotic Liver Disease Influence the Progression of End-Stage Liver DiseaseGastro Hep AdvancesCryopreserved primary hepatocytes from 123 healthy individuals were obtained from In Vitro ADMET Laboratories Inc (IVAL, Columbia, MD). These hepatocytes were isolated from liver specimens of donors who were negative for Hepatitis C virus, Hepatitis B virus, and human immunodeficiency virus. Vials of cryopreserved cells wereFull Paper
2023Imai, M;Nakamura, Y;Denda, T;Komatsu, Y;Yuki, S;Nishina, T;Hamamoto, Y;Hara, H;Esaki, T;Kawakami, H;Kato, K;Satoh, T;Okano, N;Sunakawa, Y;Taniguchi, H;Yamaguchi, K;Yamada, T;Miki, I;Wakabayashi, M;Kuwata, T;Shitara, K;Yoshino, T;Association of PD-L1 and PD-L2 expression and tumor-infiltrating lymphocytes in BRAF V600E-mutated metastatic colorectal cancer: GI-SCREEN post-hoc analysisESMO Gastrointestinal OncologyPD-L1 and PD-L2 expression was assessed by the pathologists at Qualtek Laboratories and NeoGenomics Laboratories, respectively, in a blinded manner, for baseline SaveFull Paper
2023Al-Batran, S;Roetzer, I;Wohn, L;Blau, W;Zander, T;Luley, K;Ivanyi, P;Hofheinz, R;Groschek, M;Kreiss-Sender, J;Baur, C;Wolff, T;Wenning, B;Decker-Baumann, C;Bouymayan, S;Sookthai, D;Schaaf, M;Riedel, J;Goetze, T;Haag, G;Home parenteral nutrition (HPN) using individually compounded PN via A 7/8/9-Multi-chamber system versus PN via 2/3-chamber bags in subjects with metastatic or localized solid tumors requiring HPN - The IKF-010 Pekannuss trialClinical Nutrition ESPENier, MSD Oncology, Lilly, Bristol-Myers Squibb, Targos GmbH, iOMEDICO, MCI conventions, Taiho Pharmaceutical, Nordic Group, Consultant for: Bristol-Myers Squibb, MSD Oncology, Lilly, Novartis, Daiichi Sankyo Europe GmbH, Servier, Other: Lilly, BristoFull Paper
2023Sweeney, E;Sekhri, P;Muniraj, N;Chen, J;Feng, S;Terao, J;Chin, S;Schmidt, D;Bollard, C;Cruz, C;Fernandes, R;Photothermal Prussian blue nanoparticles generate potent multi?targeted tumor?specific T cells as an adoptive cell therapyBioengineering & Translational MedicineHealthy donor peripheral blood leukopaks were purchased from AllCells (Alameda, CA) with the corresponding HLA reports. Peripheral blood mononuclear cells (PBMCs) SaveFull Paper
2023Mukhamedshin, A;Reddington, R;Dinh, M;Abhishek, K;Iqbal, M;Manheim, M;Gifford, S;Shevkoplyas, S;Rapid, label?free enrichment of lymphocytes in a closed system using a flow?through microfluidic deviceBioengineering & Translational MedicineAn additional three units from AllCells were picked up locally and processed on the day of collection. All leukapheresis samples were passed through a 40 ?m aggregate SaveFull Paper
2023Leyva Calero, A;Desarrollo y evaluación de una técnica de quimioluminiscencia para la detección de antígeno de galactomanano de Aspergillus spp. en muestras biológicas.ThesisR-409-040 LBA Discovery Life SciencesFull Paper
2023Santagata, S;Cahill, D;Curry, W;Barker, F;Dunn, IF;DF/HCC Protocol#: 17-296 TITLE: Phase II Trial of Pembrolizumab in Recurrent or Residual High Grade Meningiomaclassic.clinicaltrials.govPD-L1 expression will be measured by the QualTek assay as well as with RNA-Seq as specified below in 9.3.3. SaveFull Paper
2023Rogers, BB;Cis-Regulatory Elements: Relevance for Alzheimer's DiseaseThesisfrom each sample and the entire pool was processed according to the multiome protocol. Libraries were sequenced by HudsonAlpha Discovery using Illumina NovaSeq S4 flowcellsFull Paper
2023Kim, Y;Systems Approach to Unravel Lymphoid Development in Human Bone MarrowThesisDeidentified human bone marrow (BM) (n=9) were obtained from healthy adult donors (AllCells, Alameda, CA and StemExpress, Folsom, CA). Ex vivo labeling human bone marrow for CyTOF screen Fresh BM aspirates were labeled for their biosynthesis as previoFull Paper
2023Alluhayb, AH;COMPREHENSIVE ANALYSIS AND FORENSIC INVESTIGATION OF CYANIDE, THIOCYANATE, AND 2-AMINO-2-THIAZOLINE-4-CARBOXYLIC ACID INANTE- AND POSTMORTEM BLOOD UTILIZING ADVANCED ANALYTICALThesisirus (HCV), and Human Immunodeficiency virus (HIV). The donors had no known use of tobacco and no known history of alcohol abuse. The blood was collected by Discovery Life Sciences (Huntsville, Alabama, United States) and shipped on dry ice to the Department of Chemistry and Biochemistry at South Dakota State University. Upon receipt, all blood samples were stored at -80 °C until thawed for method development and validation. Method development of the analysis technique for whole (antemortem) blood utilized human whole blood drawn from a healthy individual caucasian maFull Paper
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2023Bellet-Ezquerra, M;Pardo, P;Fasani, R;Villacampa, G;Portu, M;Serna, G;Joval, L;Petit, A;Ortega, A;Jimenez, J;Zamora, E;Gil, M;Gomez, L;Viaplana, C;Gallego, F;Cámara, V;Manich, C;Simon, S;Nuciforo, P;62P KI67 in ER+HER2-negative pure invasive lobular breast carcinoma (ILC). What’s the best Ki67 threshold to differentiate prognosis?ESMO Opena, Novartis, Daiichi Sankyo; Non-Financial Interests, Invited Speaker: SOLTI. P.G. Nuciforo: Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal,Full Paper
2023Cieniewicz, B;Bhatta, A;Torabi, D;Baichoo, P;Saxton, M;Arballo, A;Nguyen, L;Thomas, S;Kethar, H;Kukutla, P;Shoaga, O;Yu, B;Yang, Z;Fate, M;Oliveira, E;Ning, H;Corey, L;Corey, D;Chimeric TIM-4 receptor-modified T cells targeting phosphatidylserine mediates both cytotoxic anti-tumor responses and phagocytic uptake of tumor-associated antigen for T cell cross-presentationMolecular therapy : the journal of the American Society of Gene Therapy37194236Healthy donor T cells were positively selected from fresh leukopak provided by AllCells (Alameda, CA) by using anti-CD4 and CD8 antibodies through the Prodigy TCT program. All related reagents and instruments were purchased from Miltenyi. After selecFull Paper
2023Overbeck, TR;Reiffert, A;Schmitz, K;Rittmeyer, A;Körber, W;Hugo, S;Schnalke, J;Lukat, L;Hugo, T;Hinterthaner, M;Reuter-Jessen, K;Schildhaus, HU;NTRK Gene Fusions in Non-Small-Cell Lung Cancer: Real-World Screening Data of 1068 Unselected PatientsCancers37296928r or speaker from: Amgen, AstraZeneca, Bayer, Daiichi-Sankyo, Diaceutics, Incyte, Janssen-Cilag, Eli Lilly, Merck Sharp and Dohme, Novartis Pharma, Pfizer, PharmaMar, Roche Pharma, Servier, Stemline, Targos GmbH, and ZytoVision. A.R. received grantsFull Paper
2023Wagner, R;McKindles, K;Bullerjahn, G;Effects of water movement and temperature on Rhizophydium infection of Planktothrix in a shallow hypereutrophic lakeFrontiers in Microbiologysent to HudsonAlpha Discovery Life Sciences (Hudson, AL, United States) for sequencing. Amplicon sequencing and QC was performed by HudsonAlpha Discovery Life Save;- Discovery Life Sciences (Hudson, AL, United States) for sequencing. Amplicon seFull Paper
2023de Vries, EGE;Rüschoff, J;Lolkema, M;Tabernero, J;Gianni, L;Voest, E;de Groot, DJA;Castellano, D;Erb, G;Naab, J;Donica, M;Deurloo, R;van der Heijden, MS;Viale, G;Phase II study (KAMELEON) of single-agent T-DM1 in patients with HER2-positive advanced urothelial bladder cancer or pancreatic cancer/cholangiocarcinomaCancer medicine37119523Department of Medical Oncology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.++Targos Molecular Pathology GmbH, Kassel, Germany.++Department of Medical Oncology, Erasmus MC Cancer Institute, Rotterdam, TheFull Paper
2023Wang, S;Huang, Z;Liu, Y;Sun, H;Zhou, Y;Shi, J;Sustainably released nanoparticle-based rhynchophylline limits pulmonary fibrosis by inhibiting the TEK-PI3K/AKT signaling pathwayTranslational lung cancer research37057119/tlcr-22-675/rc). Methods Solution groups For better apply to the clinic, 0.9% saline solution was used as the solvent. Because of the extremely low concentration for cell, 1 mg of Rhy (CAS 76-66-4; Allcells, Shanghai, China) was directly dissolvedFull Paper
2023Naik, NN;Vadloori, B;Poosala, S;Srivastava, P;Coecke, S;Smith, A;Akhtar, A;Roper, C;Radhakrishnan, S;Bhyravbhatla, B;Damle, M;Pulla, VK;Hackethal, J;Horland, R;Li, AP;Pati, F;Singh, MS;Occhetta, P;Bisht, R;Dandekar, P;Bhagavatula, K;Pajkrt, D;Johnson, M;Weber, T;Huang, J;Hysenaj, L;Mallar, B;Ramray, B;Dixit, S;Joshi, S;Kulkarni, M;Advances in Animal Models and Cutting-Edge Research in Alternatives: Proceedings of the Third International Conference on 3Rs Research and Progress, Vishakhapatnam, 2022Alternatives to laboratory animals : ATLA37282515Dr Li highlighted the discovery focus of Discovery Life Sciences LLC, namely the liver and intestine. He further described the properties of their two major novel human in SaveFull Paper
2023Guler, S;DiPoto, MC;Crespo, A;Caldwell, R;Doerfel, B;Grossmann, N;Ho, K;Huck, B;Jones, CC;Lan, R;Musil, D;Potnick, J;Schilke, H;Sherer, B;Simon, S;Sirrenberg, C;Zhang, Z;Liu-Bujalski, L;Selective Wee1 Inhibitors Led to Antitumor Activity In Vitro and Correlated with MyelosuppressionACS medicinal chemistry letters37197456We acknowledge Bettina Hoelke, Brian Dill, Ioannis Gounaris, and Emer Clarke (ReachBio) for their technical support and discussionsFull Paper
2023Tang, PZ;Ding, B;Reyes, C;Papp, D;Potter, J;Target-seq: single workflow for detection of genome integration site, DNA translocation and off-target eventsBioTechniques37161298Peripheral blood mononuclear cells (PBMCs) were purified from leukopak (ALLCELLS, CA, USA) using CTS™ Rotea™ Counterflow Centrifugation System (Thermo Fisher Scientific, MA, USA) and were then activated for 3 days to enrich the T cells (at 1 × 106 ceFull Paper
2023Singh, P;MSC and HSPC Coculture: Mimicking Ex Vivo Bone Marrow NicheMethods in molecular biology (Clifton, N.J.)36255702CD34+ cells with micromagnetic beads-conjugated CD34 antibody and placing with the labeled cells into a magnetic field according to the specific manual instruction. Always use fresh BM samples (within 30 h of withdrawal) for CD34+ cell isolation. HumFull Paper
2023Simmons, KT;Chan, J;Hussain, S;Rose, EL;Markham, K;Byun, TS;Panicker, S;Parry, GC;Storek, M;Anti-C1s humanized monoclonal antibody SAR445088: A classical pathway complement inhibitor specific for the active form of C1sClinical immunology (Orlando, Fla.)37149117First, 1 mL of type O- hRBCs (Allcells, Alameda, CA) was washed in 4.5 mL GVB++ by centrifuging at 600 g for 5 min. The wash step was repeated three times and the cells were resuspended to a final volume of 5 mL in GVB++ after the third washFull Paper
2023Sikkema, L;Ramírez-Suástegui, C;Strobl, DC;Gillett, TE;Zappia, L;Madissoon, E;Markov, NS;Zaragosi, LE;Ji, Y;Ansari, M;Arguel, MJ;Apperloo, L;Banchero, M;Bécavin, C;Berg, M;Chichelnitskiy, E;Chung, MI;Collin, A;Gay, ACA;Gote-Schniering, J;Hooshiar Kashani, B;Inecik, K;Jain, M;Kapellos, TS;Kole, TM;Leroy, S;Mayr, CH;Oliver, AJ;von Papen, M;Peter, L;Taylor, CJ;Walzthoeni, T;Xu, C;Bui, LT;De Donno, C;Dony, L;Faiz, A;Guo, M;Gutierrez, AJ;Heumos, L;Huang, N;Ibarra, IL;Jackson, ND;Kadur Lakshminarasimha Murthy, P;Lotfollahi, M;Tabib, T;Talavera-López, C;Travaglini, KJ;Wilbrey-Clark, A;Worlock, KB;Yoshida, M;Lung Biological Network Consortium, ;van den Berge, M;Bossé, Y;Desai, TJ;Eickelberg, O;Kaminski, N;Krasnow, MA;Lafyatis, R;Nikolic, MZ;Powell, JE;Rajagopal, J;Rojas, M;Rozenblatt-Rosen, O;Seibold, MA;Sheppard, D;Shepherd, DP;Sin, DD;Timens, W;Tsankov, AM;Whitsett, J;Xu, Y;Banovich, NE;Barbry, P;Duong, TE;Falk, CS;Meyer, KB;Kropski, JA;Pe'er, D;Schiller, HB;Tata, PR;Schultze, JL;Teichmann, SA;Misharin, AV;Nawijn, MC;Luecken, MD;Theis, FJ;An integrated cell atlas of the lung in health and diseaseNature medicine3729121419-ms2/?ds=full&meta=SampleName, https://figshare.com/articles/dataset/Single-cell_RNA-Seq_of_human_primary_lung_and_bronchial_epithelium_cells/11981034/1, https://covid19.lambrechtslab.org/downloads/Allcells.counts.rds, https://s3.amazonaws.com/dp-lFull Paper
2023Sternberg, CN;Petrylak, DP;Bellmunt, J;Nishiyama, H;Necchi, A;Gurney, H;Lee, JL;van der Heijden, MS;Rosenbaum, E;Penel, N;Pang, ST;Li, JR;García Del Muro, X;Joly, F;Pápai, Z;Bao, W;Ellinghaus, P;Lu, C;Sierecki, M;Coppieters, S;Nakajima, K;Ishida, TC;Quinn, DI;FORT-1: Phase II/III Study of Rogaratinib Versus Chemotherapy in Patients With Locally Advanced or Metastatic Urothelial Carcinoma Selected Based on FGFR1/3 mRNA ExpressionJournal of clinical oncology : official journal of the American Society of Clinical Oncology36240478ted prevalence of PIK3CA and/or RAS resistance mutations, we reconfirmed absence or presence in all enrolled patients using a targeted Illumina MiSeq panel (Illumina, Inc, San Diego, CA) performed by TARGOS Molecular Pathology GmbH (Kassel, Germany).Full Paper
2023Ho, C;Fromm, J;Fang, M;Till, B;Shadman, M;Cowan, A;Lynch, R;Wu, V;Voutsinas, J;Rasmussen, H;Blue, K;Ujjani, C;Shustov, A;Cassaday, R;Gopal, A;Smith, S;Long-term efficacy and safety of pembrolizumab with R-CHOP as first-line therapy for DLBCL.Journal of Clinical OncologyAdditionally, prognostic factors including tumor PD-L1 expression (Qualtek/Merck) were re-analyzed. Results: 30 patients were treated (13/30 with IPI 3-5). Median follow SaveFull Paper
2023Zhang, Y;Zhao, Z;Huang, LA;Liu, Y;Yao, J;Sun, C;Li, Y;Zhang, Z;Ye, Y;Yuan, F;Nguyen, TK;Garlapati, NR;Wu, A;Egranov, SD;Caudle, AS;Sahin, AA;Lim, B;Beretta, L;Calin, GA;Yu, D;Hung, MC;Curran, MA;Rezvani, K;Gan, B;Tan, Z;Han, L;Lin, C;Yang, L;Molecular mechanisms of snoRNA-IL-15 crosstalk in adipocyte lipolysis and NK cell rejuvenationCell metabolism37329887Healthy and obese human serum Coriell Biorepository See Table S1 Human breast cancer tumor tissues and paired plasma samples Discovery Life Sciences See Table S2 Primary human pre-adipocytes Zen-Bio See Table S3Full Paper
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2023Dunbar, AJ;Kim, D;Lu, M;Farina, M;Bowman, RL;Yang, JL;Park, Y;Karzai, A;Xiao, W;Zaroogian, Z;O'Connor, K;Mowla, S;Gobbo, F;Verachi, P;Martelli, F;Sarli, G;Xia, L;Elmansy, N;Kleppe, M;Chen, Z;Xiao, Y;McGovern, E;Snyder, J;Krishnan, A;Hill, C;Cordner, K;Zouak, A;Salama, ME;Yohai, J;Tucker, E;Chen, J;Zhou, J;McConnell, T;Migliaccio, AR;Koche, R;Rampal, R;Fan, R;Levine, RL;Hoffman, R;CXCL8/CXCR2 signaling mediates bone marrow fibrosis and is a therapeutic target in myelofibrosisBlood36800567ple was independently verified by a hematopathologist (W.X.) or pulled directly from clinical pathology reports at the time of sample collection. Deidentified, healthy CD34+ cells were purchased from AllCells. CD34+ selection was carried out using FiFull Paper
2023Kiridena, S;Wijayaratna, U;Levon, E;Moschella, P;Pirrallo, R;Tzeng, T;Anker, J;X?Ray Visualized Sensors for Peritoneal Dialysis Catheter InfectionAdvanced Functional MaterialsDeidentified human peritoneal fluid was obtained from Discovery Life Sciences, Huntsville, AL, a commercial biospecimen source. Tantalum beads (0.394 mm diameter) SaveFull Paper
2023Tarafder, S;Ghataure, J;Langford, D;Brooke, R;Kim, R;Eyen, SL;Bensadoun, J;Felix, JT;Cook, JL;Lee, CH;Advanced bioactive glue tethering Lubricin/PRG4 to promote integrated healing of avascular meniscus tearsBioactive materials37214259?S-encapsulating TGF?3 (R&D systems) (2.5 ?g per 500 mg PLGA) was applied to the incised tissues. Then the meniscus explants were placed on top of monolayer-cultured P2 - P3 syMSCs or hBMSCs (AllCells, Alameda, CA) at 80-90% confluence per our previoFull Paper
2023Sarno, J;Domizi, P;Liu, Y;Merchant, M;Pedersen, CB;Jedoui, D;Jager, A;Nolan, GP;Gaipa, G;Bendall, SC;Bava, FA;Davis, KL;Dasatinib overcomes glucocorticoid resistance in B-cell acute lymphoblastic leukemiaNature communications37217509Healthy human bone marrow samples (n = 3 for RNA-Seq, n = 4 for CyTOF) were purchased through AllCells. De-identified bone marrow samples from pediatric patients SaveFull Paper
2023Yang, Y;Yang, H;Alcaina, Y;Puc, J;Birt, A;Vedvyas, Y;Gallagher, M;Alla, S;Riascos, MC;McCloskey, JE;Du, K;Gonzalez-Valdivieso, J;Min, IM;de Stanchina, E;Britz, M;von Hofe, E;Jin, MM;Inducible expression of interleukin-12 augments the efficacy of affinity-tuned chimeric antigen receptors in murine solid tumor modelsNature communications37045815quoted and stored at ?80?°C. CAR-T cells were generated by transducing activated T cells with lentivirus. Primary T cells were enriched from commercially obtained leukopaks from healthy donors (AllCells) via MACS separation using CD4 (Miltenyi BioteFull Paper
2023Lee, JY;Jonus, HC;Sadanand, A;Branella, GM;Maximov, V;Suttapitugsakul, S;Schniederjan, MJ;Shim, J;Ho, A;Parwani, KK;Fedanov, A;Pilgrim, AA;Silva, JA;Schnepp, RW;Doering, CB;Wu, R;Spencer, HT;Goldsmith, KC;Identification and targeting of protein tyrosine kinase 7 (PTK7) as an immunotherapy candidate for neuroblastomaCell reports. Medicine37343516Bacterial and virus strains ------------------------- Stbl3 Competent Cells Spencer Lab N/A ------------------------- Biological samples ------------------------- Healthy PBMC AllCells LP,CR,MNC, 10M COG-424s (Neuroblastoma PDX) ChiFull Paper
2023Chen, SS;Barrientos, JC;Ferrer, G;King-Richards, M;Chen, YJ;Ravichandran, P;Ibrahim, M;Kieso, Y;Waters, S;Kutok, JL;Peluso, M;Sharma, S;Weaver, DT;Pachter, JA;Rai, KR;Chiorazzi, N;Duvelisib Eliminates CLL B Cells, Impairs CLL-Supporting Cells, and Overcomes Ibrutinib Resistance in a Xenograft ModelClinical cancer research : an official journal of the American Association for Cancer Research37071496Human PBMCs obtained from whole blood of CLL donors were purchased from a commercial source (Bioreclamation IVT or ALLCELLS) were resuspended (1 × 10 6 cells/ SaveFull Paper
2023Xiang, J;Liu, Q;Shaknovich, R;Venn, O;Abstract 779: Clonal B-cell expansion and the potential challenges to blood-based early cancer detectionCancer Researchht tubes of frozen buffy coat for a young healthy donor (18-35; no pre-existing health conditions; nonsmoker; viral negative) was sourced commercially (Discovery Life Sciences, Huntsville, AL, USA) and used as the backgrouFull Paper
2023Fahl, S;Colwell, K;Smith, C;Ott, C;Moore, A;Williams, K;Abstract 602: Single cell evaluation of intratumoral B cells across solid tumor indicationsCancer Research1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, HuntsvillFull Paper
2023Li, A;Wei, H;Abstract 5338: A novel in vitro assay to evaluate the roles of hepatic metabolism on anticancer drug safety and efficacyCancer Research1Discovery Life Sciences LLC, Columbia, MD.++1Discovery Life Sciences LLC, Columbia, MDFull Paper
2023Roy, G;Kadekar, P;Douglas, L;Frappier, M;Currie, J;Dhillon, J;Cesarone, G;Siderits, R;Kirchner, K;Demeule, M;Marsolais, C;Abstract 3942: Differential expression of a novel transport receptor, SORT1 (sortilin), in cancer versus healthy tissues that can be utilized for targeted delivery of anti-cancer drugsCancer Research1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec, Canada;++1Theratechnologies Inc., Montreal, Quebec,Full Paper
2023Cheng, C;Chen, H;Kirchner, K;Cesarone, G;Abstract 2763: Characterization of a novel immunohistochemistry (IHC) assay for CEACAM5 using a commercial antibodyCancer Research[Abcam], and 327 [Sino Biological]) to evaluate CEACAM5 detection in formalin-fixed, paraffin-embedded (FFPE) human cancer tissues from Discovery Life Sciences SaveFull Paper
2023Zielinski, D;Vogtmann, R;Siemon, A;Koppel, C;Schipper, C;Tereshchenko, A;Platero, S;Schildhaus, H;Abstract 1018: Multiplex immunofluorescence and image analysis to investigate the role of the immune contexture and fibroblast activation for tumor cell budding in colorectal cancerCancer Research1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, Huntsville, AL.++1Discovery Life Sciences, HuntsvillFull Paper
2023Kumar, A;Taghi Khani, A;Duault, C;Aramburo, S;Sanchez Ortiz, A;Lee, SJ;Chan, A;McDonald, T;Huang, M;Lacayo, NJ;Sakamoto, KM;Yu, J;Hurtz, C;Carroll, M;Tasian, SK;Ghoda, L;Marcucci, G;Gu, Z;Rosen, ST;Armenian, S;Izraeli, S;Chen, CW;Caligiuri, MA;Forman, SJ;Maecker, HT;Swaminathan, S;Intrinsic suppression of type I interferon production underlies the therapeutic efficacy of IL-15-producing natural killer cells in B-cell acute lymphoblastic leukemiaJournal for immunotherapy of cancer37217248opoietic Tissue Biorepository and University of Pennsylvania Stem Cell and Xenograft Core after informed consent per Institutional Review Board policies. Age-matched healthy BMMCs were purchased from AllCells (Alameda, California, USA) and Stem CellFull Paper
2023Nirschl, CJ;Brodkin, HR;Domonkos, C;Dwyer, CJ;Hicklin, DJ;Ismail, N;Seidel-Dugan, C;Steiner, P;Steuert, Z;Sullivan, JM;Winston, WM;Salmeron, A;mWTX-330, an IL 12 INDUKINE Molecule, Activates and Reshapes Tumor-infiltrating CD8+ T and NK Cells to Generate Antitumor ImmunityCancer immunology research37074216Dissociated human tumor samples were purchased from Discovery Life Sciences and included the following indications: bladder cancer, glioblastoma multiforme, gastric SaveFull Paper
2023Diwanji, R;O'Brien, NA;Choi, JE;Nguyen, B;Laszewski, T;Grauel, AL;Yan, Z;Xu, X;Wu, J;Ruddy, DA;Piquet, M;Pelletier, MR;Savchenko, A;Charette, L;Rodrik-Outmezguine, V;Baum, J;Millholland, JM;Wong, CC;Martin, AM;Dranoff, G;Pruteanu-Malinici, I;Cremasco, V;Sabatos-Peyton, C;Jayaraman, P;Targeting the IL1? Pathway for Cancer Immunotherapy Remodels the Tumor Microenvironment and Enhances Antitumor Immune ResponsesCancer immunology research37040466Dissociated human microsatellite-stable colorectal carcinoma (MSS-CRC) samples were obtained from Discovery Life Sciences from five donors. Between 25,000 and SaveFull Paper
2023Huang, W;Lin, W;Chen, B;Zhang, J;Gao, P;Fan, Y;Lin, Y;Wei, P;NFAT and NF-?B dynamically co-regulate TCR and CAR signaling responses in human T cellsCell reports37347664K562 Laboratory of Wendell Lim N/A Jurkat Laboratory of Arthur Weiss N/A Human PBMC ALLCELLS PB004F-CFull Paper
2023Raj, N;Chan, JA;Wang, SJ;Aggarwal, RR;Calabrese, S;DeMore, A;Fong, L;Grabowsky, J;Hope, TA;Kolli, KP;Mulvey, CK;Munster, PN;Perez, K;Punn, S;Reidy-Lagunes, D;Von Fedak, S;Zhang, L;Bergsland, EK;Pembrolizumab alone and pembrolizumab plus chemotherapy in previously treated, extrapulmonary poorly differentiated neuroendocrine carcinomasBritish journal of cancer37208512Background To date, single-agent immune checkpoint inhibitor (CPI) therapy has proven to be ineffective against biomarker-unselected extrapulmonary poorly SaveFull Paper
2023Reissig, TM;Tzianopoulos, I;Liffers, ST;Rosery, VK;Guyot, M;Ting, S;Wiesweg, M;Kasper, S;Meister, P;Herold, T;Schmidt, HH;Schumacher, B;Albers, D;Markus, P;Treckmann, J;Schuler, M;Schildhaus, HU;Siveke, JT;Smaller panel, similar results: genomic profiling and molecularly informed therapy in pancreatic cancerESMO open37148593stock ownership: none; honoraries for CME presentations: Amgen, Boehringer Ingelheim, Bristol-Myers Squibb, Janssen, Novartis; research funding to institution: AstraZeneca, Bristol Myers-Squibb. HUS: Targos Molecular Pathology, Inc. (employment); RocFull Paper
2023Han, R;Guo, H;Shi, J;Wang, H;Zhao, S;Jia, Y;Liu, X;Li, J;Cheng, L;Zhao, C;Li, X;Zhou, C;Tumour microenvironment changes after osimertinib treatment resistance in non-small cell lung cancerEuropean journal of cancer (Oxford, England : 1990)37320935Fresh PBMCs of three healthy donors were purchased from Allcells (China). A total of 2 × 10 6 PBMC cells/well were seeded into 24-well plated and were treated with: SaveFull Paper
2023Geoerger, B;Marshall, LV;Nysom, K;Makin, G;Bouffet, E;Defachelles, AS;Amoroso, L;Aerts, I;Leblond, P;Barahona, P;Van-Vlerken, K;Fu, E;Solca, F;Lorence, RM;Ziegler, DS;Afatinib in paediatric patients with recurrent/refractory ErbB-dysregulated tumours: Results of a phase I/expansion trialEuropean journal of cancer (Oxford, England : 1990)37178647Biomarker assays were performed centrally by TARGOS Molecular Pathology GmbH using tissue samples from archived tumour blocks, or tissue sections [20]. SaveFull Paper
2023Robert, ME;Rüschoff, J;Jasani, B;Graham, RP;Badve, SS;Rodriguez-Justo, M;Kodach, LL;Srivastava, A;Wang, HL;Tang, LH;Troncone, G;Rojo, F;Van Treeck, BJ;Pratt, J;Shnitsar, I;Kumar, G;Karasarides, M;Anders, RA;Erratum to High Interobserver Variability Among Pathologists Using Combined Positive Score to Evaluate PD-L1 Expression in Gastric, Gastroesophageal Junction, and Esophageal Adenocarcinoma [Modern Pathology 36(5) (2023) 100154]Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc37327723Yale University School of Medicine, New Haven, Connecticut. Electronic address: marie.robert@yale.edu.++Discovery Life Sciences, Hesse, Germany.++Discovery Life Sciences, Hesse, Germany.++Mayo Clinic, Rochester, Minnesota.++Emory University School ofFull Paper
2023Dong, Q;Tong, M;Yu, X;Wang, L;Ao, J;Guan, D;Tang, Y;Liu, J;Long, L;Tong, Y;Fang, S;Zhou, H;Huang, Y;Gong, L;Lou, L;Huang, W;Carbohydrate Strengthens the Immunotherapeutic Effect of Small-Molecule PD-L1 InhibitorsJournal of medicinal chemistry37226718Stimulator cells from donor A and responder cells from donor B constitute the mixed lymphocyte reaction (MLR). Pheral Blood Mononuclear Cells (PBMCs) were purchased from Allcells (Shanghai) and used following the instruction of the manufacturer. DC cFull Paper
2023Papakonstantinou, A;Napoli, S;Joval, L;Butjosa-Espin, M;Malavila, A;Puy, M;Pimentel, I;Zamora, E;Torres, P;Rodas, N;Manich, C;Seoane, J;Nonell, L;Nuciforo, P;Oliveira, M;19P Tumor-associated microbiome composition and response to neoadjuvant chemotherapy (NACT) in early triple-negative breast cancer (TNBC)ESMO Openncer Research (AACR). P.G. Nuciforo: Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. M. OFull Paper
2023Akiyama, H;Carter, BZ;Andreeff, M;Ishizawa, J;Molecular Mechanisms of Ferroptosis and Updates of Ferroptosis Studies in Cancers and LeukemiaCells37190037Another study demonstrates that progestin and adipoQ receptor 3 (PAQR3) enhances erastin- as well as RSL3-induced ferroptosis in ALLcells through the degradation of SaveFull Paper
2023Chaand, M;Fiore, C;Johnston, B;D'Ippolito, A;Moon, DH;Carulli, JP;Shearstone, JR;Erythroid lineage chromatin accessibility maps facilitate identification and validation of NFIX as a fetal hemoglobin repressorCommunications biology37316562Fetal CB-derived CD34+ cells (AllCells, LLC) were transduced with concentrated lentivirus at an MOI = 1. Transduced cells were selected in the presence of 2 µg/mL SaveFull Paper
2023Lin, W;Singh, V;Springer, R;Choonoo, G;Gupta, N;Patel, A;Frleta, D;Zhong, J;Owczarek, T;Decker, C;Macdonald, L;Murphy, A;Thurston, G;Mohrs, M;Ioffe, E;Lu, YF;Human CD4 cytotoxic T lymphocytes mediate potent tumor control in humanized immune system miceCommunications biology37185301from fetal liver or cord blood, respectively. Fetal liver CD34+ HSPC were obtained from Advanced Biosciences Resources (Alameda, CA) with proper consent66 and cord blood CD34+ HSPC were obtained from AllCells, HemaCare, or STEMCELL Technologies. HumaFull Paper
2023Maden, M;Polvadore, T;Polanco, A;Barbazuk, WB;Stanley, E;Osteoderms in a mammal the spiny mouse Acomys and the independent evolution of dermal armoriScience37378333na NovaSeq6000 platform to yield nearly 898 million paired-end, 150 bp reads (mean of 74.8 M read pairs (s = 9.1 M read pairs) per library). Library construction and sequencing were performed by HudsonAlpha Discovery in Huntsville, Alabama, USA. QuaFull Paper
2023Egelston, CA;Guo, W;Yost, SE;Ge, X;Lee, JS;Frankel, PH;Cui, Y;Ruel, C;Schmolze, D;Murga, M;Tang, A;Martinez, N;Karimi, M;Somlo, G;Lee, PP;Waisman, JR;Yuan, Y;Immunogenicity and efficacy of pembrolizumab and doxorubicin in a phase I trial for patients with metastatic triple-negative breast cancerCancer immunology, immunotherapy : CII37294342Biomarker Working Group on Breast Cancer Guidelines [18]. PD-L1 was determined by QualTek Molecular Laboratory (Goleta, CA) using immunohistochemistry (IHC) with 22C3 antibody (Merck & Co, Kenilworth, NJ). PD-L1 was positive if membrane staining wasFull Paper
2023Becker, SA;Petrich, BG;Yu, B;Knight, KA;Brown, HC;Raikar, SS;Doering, CB;Spencer, HT;Enhancing the effectiveness of ?? T cells by mRNA transfection of chimeric antigen receptors or bispecific T cell engagersMolecular therapy oncolytics37387794ed from healthy donor blood through the Children’s Clinical Translational Discovery Core at Emory University under the core’s approved institutional review board protocol or ordered directly from AllCells. PBMCs were isolated from fresh blood using FFull Paper
2023Chervin, AS;Stone, JD;Konieczna, I;Calabrese, KM;Wang, N;Haribhai, D;Dong, F;White, MK;Rodriguez, LE;Bukofzer, GT;Ellis, PA;Cosgrove, C;Hecquet, C;Clarin, JD;Palma, JP;Reilly, EB;ABBV-184: A novel survivin-specific TCR/CD3 bispecific T cell engager is active against both solid tumor and hematological malignanciesMolecular cancer therapeutics37294945Primary frozen viable PBMCs from AML patients and including AML blasts were purchased from Discovery Life Sciences and verified for HLA-A2 expression by staining with the HLA-A2 specific monoclonal antibody BB7.2 and detecting by flow cytometryFull Paper
2023Korntner, SH;Di Nubila, A;Gaspar, D;Zeugolis, DI;Macromolecular crowding in animal component-free, xeno-free and foetal bovine serum media for human bone marrow mesenchymal stromal cell expansion and differentiationFrontiers in bioengineering and biotechnology36949882ed. 2.2 Isolation and expansion of hBMSCs Fresh human whole bone marrow from the iliac crest of two different donors (donor 1: female, 22 years old; donor 2: male, 25 years old) was purchased from AllCells (United States) and hBMSCs were isolated uFull Paper
2023Dempsey, MP;Andersen, KE;Wells, BM;Taylor, MA;Cashman, CL;Conrad, LB;Kearney, CA;Conklin, MB;Via, ER;Doe, EM;Komirisetty, R;Dearborn, S;Reddy, ST;Farias-Eisner, R;Stability Characterization of the Novel Anti-Cancer HM-10/10 HDL-Mimetic PeptideInternational journal of molecular sciences3737320382043-96OD), one plate per lot, were obtained from In Vitro ADMET Laboratories (Malden, MA, USA). The transport medium was replaced with 200 ?L of warm (37 C) SaveFull Paper
2023El Ouardi, M;Tamarit, L;Vayá, I;Miranda, MA;Andreu, I;Cellular photo(geno)toxicity of gefitinib after biotransformationFrontiers in pharmacology37351506UPV-CSIC, Universitat Politècnica de València, Valencia, Spain 2 Unidad Mixta de Investigación UPV- IIS La Fe, Hospital Universitari i Politècnic La Fe, Valencia, Spain Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States Reviewed byFull Paper
2023Takei, S;Kinoshita, H;Jamal, M;Kumihashi, M;Yamashita, T;Tanaka, E;Kawahara, S;Abe, H;Tsutsui, K;Kimura, S;Case report: Fatal poisoning caused by additive effects of two barbituratesFrontiers in pharmacology37292155Medicine, Faculty of Medicine, Kagawa University, Takamatsu, Japan 2 Biodesign Inc, Tokyo, Japan 3 Kagawa Prefectural University of Health Sciences, Takamatsu, Kagawa, Japan Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States ReviewFull Paper
2023Li, S;Zhan, J;Wang, Y;Oduro, PK;Owusu, FB;Zhang, J;Leng, L;Li, R;Wei, S;He, J;Wang, Q;Suxiao Jiuxin Pill attenuates acute myocardial ischemia via regulation of coronary artery toneFrontiers in pharmacology37234713vestigated. The EC50 and Rmax (maximal vasorelaxation) values were then calculated. 2.8 Cell culture and western blotting analysis Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells Biotech Co., Ltd. (Shanghai, China). TheFull Paper
2023Greenstein, AE;Hunt, HJ;The glucocorticoid receptor modulator relacorilant reverses the immunosuppressive effects of cortisolInternational immunopharmacology37230031GR was assessed using a rabbit monoclonal antibody clone D8H2 from Cell Signaling Technology (Cat#3660S) as described previously [41]. CD3 (Dako, A0452), FOXP3 (eBioscience 14-4777), CD57 (ThermoFisher, MA5-12008), and PD-1 (Abcam, ab53587) were acquFull Paper
2023Cyr, MG;Wilson, HD;Spierling, AL;Chang, J;Peng, H;Steinberger, P;Rader, C;Concerted Antibody and Antigen Discovery by Differential Whole-cell Phage Display Selections and Multi-omic Target DeconvolutionJournal of molecular biology37019174Fresh healthy donor PBMCs were bought from AllCells and cryopreserved prior to use with 50% FBS, 10% DMSO in RPMI-1640 medium. SaveFull Paper
2023Isaioglou, I;Aldehaiman, MM;Li, Y;Lahcen, AA;Rauf, S;Al-Amoodi, AS;Habiba, U;Alghamdi, A;Nozue, S;Habuchi, S;Salama, KN;Merzaban, JS;CD34+ HSPCs-derived exosomes contain dynamic cargo and promote their migration through functional binding with the homing receptor E-selectinFrontiers in cell and developmental biology37181754O2 concentration of 5% v/v. The cell cultures were passaged frequently to maintain the cells at 0.8 × 106 cells/ml. Primary human AML CD34+ cells from Mobilized Peripheral Blood were purchased from AllCells, while Primary Human CD34+ cells from MobiFull Paper
2023Basu, A;Champagne, RN;Patel, NG;Nicholson, ED;Weiss, RJ;TFCP2 is a transcriptional regulator of heparan sulfate assembly and melanoma cell growthThe Journal of biological chemistry37061003in Table S4. RNA sequencing and differential gene expression analysis Total RNA extracted from wildtype and knockout cell lines was submitted for library preparation and next-generation sequencing (HudsonAlpha Discovery). Raw RNA sequencing data wasFull Paper
2023Spriano, F;Aresu, L;Cascione, L;Risi, G;Arribas, AJ;Napoli, S;Forster-Gross, N;Bachmann, F;Engelhardt, M;Lane, H;Bertoni, F;The microtubule-targeted agent lisavanbulin (BAL101553) shows anti-tumor activity in lymphoma modelsAmerican journal of cancer research37293172(TMA) (LY1001D and LY2086B, TissueArray.com) were stained for EB1 using a CE-marked immunohistochemistry Clinical Trial Assay (Discovery Life Sciences Biomarker SaveFull Paper
2023Baraibar, I;García, A;Salvà, F;Ros, J;Saoudi, N;Comas, R;Castillo, G;Sanchis, M;García-Álvarez, A;Hernando, J;Capdevila, J;Castells, MR;Martí, M;Landolfi, S;Espín, E;Navalpotro, B;Guevara, J;Dopazo, C;Nuciforo, P;Vivancos, A;Tabernero, J;Élez, E;Impact of the COVID-19 pandemic in the early-onset colorectal cancerTranslational oncology37031602Paolo Nuciforo reports personal financial interest in form of scientific consultancy role for Targos Molecular Pathology. His-institution has received research funding from Save;- Paolo Nuciforo reports personal financial interest in form of scieFull Paper
2023Pousse, L;Korfi, K;Medeiros, BC;Berrera, M;Kumpesa, N;Eckmann, J;Hutter, IK;Griesser, V;Karanikas, V;Klein, C;Amann, M;CD25 targeting with the afucosylated human IgG1 antibody RG6292 eliminates regulatory T cells and CD25+ blasts in acute myeloid leukemiaFrontiers in oncology37205201Frozen healthy BMMC were purchased from Discovery Life Sciences. All human samples were collected under the approval of the relevant Institutional Review Board (IRB SaveFull Paper
2023Kourula, S;Derksen, M;Jardi, F;Jonkers, S;van Heerden, M;Verboven, P;Theuns, V;Van Asten, S;Huybrechts, T;Kunze, A;Frazer-Mendelewska, E;Lai, KW;Overmeer, R;Roos, JL;Vries, RGJ;Boj, SF;Monshouwer, M;Pourfarzad, F;Snoeys, J;Intestinal organoids as an in vitro platform to characterize disposition, metabolism, and safety profile of small moleculesEuropean journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences37244450Intestinal organoids derived from LGR5 + adult stem cells allow for long-term culturing, more closely resemble human physiology than traditional intestinal models, like Save;- HQM Incubation Medium and co-factor N were acquired from in vitro ADMEFull Paper
2023Gnanapragasam, MN;Planutis, A;Glassberg, JA;Bieker, JJ;Identification of a genomic DNA sequence that quantitatively modulates KLF1 transcription factor expression in differentiating human hematopoietic cellsScientific reports37165057is human GRCh37/hg19 genome browser (https://main.genome-browser.bx.psu.edu/index.html), focusing on results using CD34+ or bone marrow derived erythroid cells. Human CD34+ cells were purchased from AllCells or obtained from the Yale Cooperative CenFull Paper
2023Li, J;Zhang, Z;Zhuang, Y;Wang, F;Cai, T;Small RNA transcriptome analysis using parallel single-cell small RNA sequencingScientific reports37160973ng). PBMCs preparation Cryopreserved CD3+ Pan T cells (PB009-1F-C-5M), CD19+ B cells (PB010-P-F-C), CD14+ monocytes (PB-011-P-F-1-C), and CD56+ natural killer cells (PB012-P-F-C) were purchased from AllCells (Shanghai, China). The CD3+ Pan T cells wFull Paper
2023Ke, H;Zhang, F;Wang, J;Xiong, L;An, X;Tu, X;Chen, C;Wang, Y;Mao, B;Guo, S;Ju, C;He, X;Sun, R;Zhang, L;O'Connor, OA;Li, QX;HX009, a novel BsAb dual targeting PD1 x CD47, demonstrates potent anti-lymphoma activity in preclinical modelsScientific reports37012357Gibco). Jurkat-CSR cells, from ImmuneOnco Biopharmaceuticals (Shanghai), were cultured in RPMI-1640 (Gibco) with 10% FBS (Gemini). Human PBMCs used in the studies were purchased from ALLCELL (https://allcells.com) where informed consent and approvedFull Paper
2023Adhikary, G;Heipertz, EL;Preradovic, M;Chen, X;Xu, W;Newland, JJ;Kaur, N;Vemuri, MC;Eckert, RL;Natural killer cells suppress human cutaneous squamous cell carcinoma cancer cell survival and tumor growthMolecular carcinogenesis36994661Cryopreserved peripheral blood mononuclear cells (PBMCs) were obtained from AllCells. NK cells were enriched via negative selection using the Human NK Cell SaveFull Paper
2023Sweeney, EE;Sekhri, P;Telaraja, D;Chen, J;Chin, SJ;Chiappinelli, KB;Sanchez, CE;Bollard, CM;Cruz, CRY;Fernandes, R;Engineered tumor-specific T cells using immunostimulatory photothermal nanoparticlesCytotherapy37278683ix-coated plates in Astrocyte Medium (Thermo Fisher Scientific). Cells were expanded as per the specifications provided by the supplier. Healthy donor peripheral blood leukopaks were purchased from AllCells (Alameda, CA, USA) with the corresponding hFull Paper
2023Zhang, L;Yu, L;Xu, Y;Qin, P;Shen, P;Liu, K;Fei, M;Wang, H;Cao, Y;Lu, L;Gao, W;Zhang, Z;Demonstrating analytical similarity of a biosimilar HLX04 to bevacizumab with a panel of state-of-the-art methods and tiering of quality attributesAnalytical and bioanalytical chemistry37162525Human umbilical vein endothelial cell (HUVEC), obtained from Allcells (No. H-001F, CA, USA), was used to determine the inhibitory effects of HLX04 and CN-/EU-Avastin® on proliferation of cells expressing VEGFR. HUVEC expresses VEGFR that binds to VEGFull Paper
2023Verner, EL;Jackson, JB;Severson, E;Valkenburg, KC;Greer, AE;Riley, DR;Sausen, M;Maddox, C;McGregor, PM;Karandikar, A;Hastings, SB;Previs, RA;Reddy, VP;Jensen, TJ;Ramkissoon, SH;Validation of the Labcorp Plasma Focus Test to Facilitate Precision Oncology Through Cell-Free DNA Genomic Profiling of Solid TumorsThe Journal of molecular diagnostics : JMD37068734Clinical samples used to evaluate the analytical performance of the Labcorp Plasma Focus test were sourced from multiple biorepositories or as part of internal sample collections (BioIVT, Chestertown, MD; Discovery Life Sciences, Huntsville, AL; PrecFull Paper
2023Ding, H;Yao, B;Ci, L;Feng, J;Ouyang, P;Chen, G;Hui, X;Zhou, D;Enhancing the Anti-tumor Potency of a Novel Siglec-15 Antibody by Engineering its Fc-mediated Effector FunctionsJournal of immunotherapy (Hagerstown, Md. : 1997)37103472Total PBMCs (AllCells) from a healthy human donor were resuspended in cell culture media (RPMI1640 + 10% FBS) at a density of 150,000 cells per well in triplicate. SaveFull Paper
2023Ekiciler, A;Chen, WLK;Bo, Y;Pugliano, A;Donzelli, M;Parrott, N;Umehara, K;Quantitative Cytochrome P450 3A4 Induction Risk Assessment Using Human Hepatocytes Complemented with Pregnane X Receptor-Activating ProfilesDrug metabolism and disposition: the biological fate of chemicals36460477on 24- or 96-well collagen I-coated plates at 0.6 or 0.06 × 10 6 cells/well, respectively, using high viability cryo-hepatocyte recovery kits (In Vitro ADMET Laboratories, LLC; SaveFull Paper
2023Bell-Glenn, S;Salas, LA;Molinaro, AM;Butler, RA;Christensen, BC;Kelsey, KT;Wiencke, JK;Koestler, DC;Calculating detection limits and uncertainty of reference-based deconvolution of whole-blood DNA methylation dataEpigenomics37337720Cells used to isolate the DNA to create the mixtures were purchased from AllCells corporation (CA, USA), StemExpress (CA, USA), and STEMCELL Technologies ( SaveFull Paper
2023Banerjee, P;Diniz, WJS;Rodning, SP;Dyce, PW;miRNA expression profiles of peripheral white blood cells from beef heifers with varying reproductive potentialFrontiers in genetics37234872The quality-checked libraries were pooled and sequenced in the NextSeq 500 using the single-end 50 bp chemistry at Discovery Life Sciences (Hudson Alpha Institute of Save;nt Bioanalyzer high-sensitivity DNA assay (Agilent, Santa Clara, CA, United SFull Paper
2023Lamothe, RC;Storlie, MD;Espinosa, DA;Rudlaff, R;Browne, P;Liu, J;Rivas, A;Devoto, A;Oki, J;Khoubyari, A;Goltsman, DSA;Lin, JL;Butterfield, CN;Brown, CT;Thomas, BC;Cost, GJ;Novel CRISPR-Associated Gene-Editing Systems Discovered in Metagenomic Samples Enable Efficient and Specific Genome EngineeringThe CRISPR journal37219969oporation, cells were harvested for flow cytometry and genomic DNA extraction as described previously. Primary HSC culture and nucleofection Mobilized peripheral blood CD34+ cells were acquired from AllCells and cultured in StemCell StemSpan SFEM IFull Paper
2023Alexander, GE;Lin, W;Ortega, FE;Ramaiah, M;Jung, B;Ji, L;Revenkova, E;Shah, P;Croisetiere, C;Berman, JR;Eubank, L;Naik, G;Brooks, J;Mich, A;Shojaee, S;Ronaghi, N;Chawla, H;Hou, X;Liu, Q;Yakym, CAV;Moradi, PW;Halks-Miller, M;Aravanis, AM;Parpart-Li, S;Hunkapiller, N;Analytical validation of a multi-cancer early detection test with cancer signal origin using a cell-free DNA-based targeted methylation assayPloS one37058491Six non-cancer samples were obtained from Discovery Life Sciences (Huntsville, AL; catalog #s 220130 and 220140) and 6 cancer samples were obtained from Save;ncer) across dilution series created by spiking cfDNA from cancer samples into cfDNA derivFull Paper
2023Li, X;Le, Y;Seo, JE;Guo, X;Li, Y;Chen, S;Mittelstaedt, RA;Moore, N;Guerrero, S;Sims, A;King, ST;Atrakchi, AH;McGovern, TJ;Davis-Bruno, KL;Keire, DA;Elespuru, RK;Heflich, RH;Mei, N;Revisiting the mutagenicity and genotoxicity of N-nitroso propranolol in bacterial and human in vitro assaysRegulatory toxicology and pharmacology : RTP37210026National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, 72079, USA. Electronic address: xilin.li@fda.hhs.gov.++National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR, 72079,Full Paper
2023Coleman, R;Metastasis prevention with bone-targeted agents: a complex interaction between the microenvironment and tumour biologyJournal of bone and mineral metabolism37162605amplification status was blinded and followed pre-specified sample handling and standard operating methodology by a central laboratory (Targos Molecular Pathology, SaveFull Paper
2023Kliver, S;Houck, ML;Perelman, PL;Totikov, A;Tomarovsky, A;Dudchenko, O;Omer, AD;Colaric, Z;Weisz, D;Aiden, EL;Chan, S;Hastie, A;Komissarov, A;Ryder, OA;Graphodatsky, A;Johnson, WE;Maldonado, JE;Pukazhenthi, BS;Marinari, PE;Wildt, DE;Koepfli, KP;Chromosome-length genome assembly and karyotype of the endangered black-footed ferret (Mustela nigripes)The Journal of heredity37249392The black-footed ferret (Mustela nigripes) narrowly avoided extinction to become an oft-cited example of the benefits of intensive management, research, and collaboration SaveFull Paper
2023Martha, L;Nakata, A;Furuya, S;Liu, W;Zhang, X;Mizoi, K;Ogihara, T;Transporter and metabolic enzyme-mediated intra-enteric circulation of SN-38, an active metabolite of irinotecan: A new conceptBiochemical and biophysical research communications37148742sages 72-83 were used. Human cryopreserved suspension enterocytes (Lot. HE3072, male, 55 years old, Caucasian) were purchased from In Vitro ADMET Laboratories (MD, USA). All other reagents and solvents were commercial products of special gradeFull Paper
2023He, S;Liu, J;Hu, L;Zhan, Y;Tong, H;Zhu, H;Guo, H;Sun, H;Liu, M;Design, synthesis, biological evaluation and molecular docking studies of quercetin-linker-H2S donor conjugates for the treatment of diabetes and wound healingChemistry & biodiversity37329234All cell culture materials were obtained from Gibco. HepG2 cells were cryopreserved in our laboratory and used after resuscitation. HUVECs cells were purchased from Shanghai Allcells Co., LtdFull Paper
2023Thompson, EN;Carlino, MJ;Scanlon, VM;Grimes, HL;Krause, DS;Assay optimization for the objective quantification of human multilineage colony-forming unitsExperimental hematology37271449Cells were derived commercially (AllCells, Alameda, CA) or were derived from healthy donors who were already donating cells for allogeneic transplantation provided SaveFull Paper
2023Goda, C;Kolovich, S;Rudich, A;Karunasiri, M;Kulkarni, R;Rajgolikar, G;Neidemire-Colley, L;Singh, S;Sircar, A;Ranganathan, P;Garzon, R;Sehgal, L;Dorrance, AM;Epidermal growth factor-like 7 is a novel therapeutic target in mantle cell lymphomaExperimental hematology37209901Full Paper
2023Yi, PC;Zhuo, L;Lin, J;Chang, C;Goddard, A;Yoon, OK;Impact of delayed PBMC processing on functional and genomic assaysJournal of immunological methods37353001An additional five whole blood samples from healthy adults were sourced from AllCells (Alameda, CA, USA), all of whom provided informed consent, under their IRB- SaveFull Paper
2023Chen, Z;Javed, N;Moore, M;Wu, J;Sun, G;Vinyard, M;Collins, A;Pinello, L;Najm, FJ;Bernstein, BE;Integrative dissection of gene regulatory elements at base resolutionCell genomics37388913us Biologicals Cat#NB100-1800 TAL1 antibody SantaCruz Biotech Cat#sc-393287 Cell lines and primary cells Jurkat cell line, Clone E6.1 ATCC Cat#TIB152, RRID:CVCL_0367 CD4+ T cells AllCells N/A ------------------------- ChemicalFull Paper
2023Kessler, ER;Callihan, E;Hu, J;Eule, C;Srivastava, G;Kemme, DJ;Iruku, P;Rana, V;Moore, J;Schuster, SR;Amirault, M;Flaig, TW;Lam, ET;A Phase I/II Clinical Trial of Pembrolizumab and Cabozantinib in Metastatic Renal Cell CarcinomaCancer research communications37377613Full Paper
2023Farabaugh, CS;Doak, S;Roy, S;Elespuru, R;In vitro micronucleus assay: Method for assessment of nanomaterials using cytochalasin BFrontiers in toxicology37180488Urbana?Champaign, Champaign, United States Khaled Habas, University of Bradford, United Kingdom *Correspondence: Rosalie Elespuru, rosalie.elespuru@dls.com † Present address: Rosalie Elespuru, Discovery Life Sciences, Columbia, MD, United StatesFull Paper
2023Baro-Sastre, B;Kim, CY;Lin, C;Kongsomboonvech, AK;Tetard, M;Salinas, ND;Tolia, NH;Egan, ES;Plasmodium falciparum exploits CD44 as a co-receptor for erythrocyte invasionbioRxiv : the preprint server for biology37090581Generation of cRBCs was performed essentially as previously described 37. Briefly, bone marrow primary human CD34+ HSPCs (Stem Cell Technologies; AllCells) were cultured in PIMDM composed of Iscove Basal Medium (Sigma) with 4 mM L-Glutamine, 330 µg/mFull Paper
2023Okano, F;Saito, T;Minamida, Y;Kobayashi, S;Ido, T;Miyauchi, Y;Wasai, U;Akazawa, D;Kume, M;Ishibashi, M;Jiang, K;Aicher, A;Heeschen, C;Yonehara, T;Identification of Membrane-expressed CAPRIN-1 as a Novel and Universal Cancer Target, and Generation of a Therapeutic Anti-CAPRIN-1 Antibody TRK-950Cancer research communications37082579ng formula based on the Cr-51 dose released from the target cells: The ADCC activity of TRK-950 via human and monkey PBMCs was conducted at Translational Drug Development (TD2). We used human PBMCs (AllCells, catalog no. PB003F) and Cynomolgus MonkeFull Paper
2023Montalban-Bravo, G;Ma, F;Thongon, N;Yang, H;Gomez, IG;Rodriguez-Sevilla, JJ;Adema, V;Wildeman, B;Lockyer, P;Kim, YJ;Tanaka, T;Darbaniyan, F;Pancholy, S;Zhang, G;Al-Atrash, G;Dwyer, K;Takahashi, K;Garcia-Manero, G;Kantarjian, H;Colla, S;Targeting MCL1-driven anti-apoptotic pathways to overcome hypomethylating agent resistance in RAS -mutated chronic myelomonocytic leukemiabioRxiv : the preprint server for biology37066354RAS pathway mutations, which are present in 30% of patients with chronic myelomonocytic leukemia (CMML) at diagnosis, confer a high risk of resistance to and SaveFull Paper
2023Dillon, LW;Higgins, J;Nasif, H;Othus, M;Beppu, L;Smith, TH;Schmidt, E;Valentine, CC;Salk, JJ;Wood, BL;Erba, HP;Radich, JP;Hourigan, CS;Quantification of measurable residual disease using duplex sequencing in adults with acute myeloid leukemiamedRxiv : the preprint server for health sciences37034683The presence of measurable residual disease (MRD) is strongly associated with treatment outcomes in acute myeloid leukemia (AML). Despite the correlation with clinical SaveFull Paper
2023Flümann, R;Hansen, J;Pelzer, BW;Nieper, P;Lohmann, T;Kisis, I;Riet, T;Kohlhas, V;Nguyen, PH;Peifer, M;Abedpour, N;Bosco, G;Thomas, RK;Kochanek, M;Knüfer, J;Jonigkeit, L;Beleggia, F;Holzem, A;Büttner, R;Lohneis, P;Meinel, J;Ortmann, M;Persigehl, T;Hallek, M;Calado, DP;Chmielewski, M;Klein, S;Göthert, JR;Chapuy, B;Zevnik, B;Wunderlich, FT;von Tresckow, B;Jachimowicz, RD;Melnick, AM;Reinhardt, HC;Knittel, G;Distinct Genetically Determined Origins of Myd88/BCL2-Driven Aggressive Lymphoma Rationalize Targeted Therapeutic Intervention StrategiesBlood cancer discovery36346827Roche, Cofounder and CSO of Targos MP Inc. (until May 2021), Gnothis Inc/Stockholm (Member of Board of Directors and Coowner). D.P. Calado reports other support from Cancer Research UK (CC2078), Wellcome Trust (CC2078), and UK Medical Research CounciFull Paper
2023Mark W., R;Joanne, W;Mary Jo, K;Melissa E., M;John V., L;Peter, F;Sleep Disturbances and Chronic Pain as Potential Indicators for Therapeutic Intervention in Subjects with Post-Traumatic Stress DisorderJournal of Depression and Anxiety Disorderscruited to an age and gender-matched case-control study (N = 78; control n = 39; PTSD n = 39). Participants were recruited in the US between January and June 2019 by PrecisionMed (California, US) and Discovery Life Sciences (California, US). A compreFull Paper
2023Buckley, D;Tew, B;Gooden, G;Salhia, B;Whole genome bisulfite sequencing of tumor DNA and matching cfDNA in relapsed pediatric cancerResearch Squarefrom Conversant Bio (Table 1). Twelve additional cfDNA healthy controls from adults were included for model building in cfDNA, detailed below (Conversant Bio). Patients SaveFull Paper
2023Ridzuan, A;Sebastian, A;Noorazman, A;Kannan, T;Syed, N;Nurul, A;Interleukin-17A enhances osteogenic differentiation by activating ERK/MAPK in stem cells derived from human exfoliated deciduous teethBiomedical Research and Therapythe participation of the MAPK signaling pathway in SHED osteogenic differentiation. MATERIALS AND METHODS CELL CULTURE AND OSTEOGENIC DIFFERENTIATION IN VITRO The SHED cell line, obtained from ALLCELLS (Alameda, CA, USA), was derived from humaFull Paper
2023Pillsbury, C;Dougan, J;Rabe, J;Fonseca, J;Zhou, C;Evans, A;Abukharma, H;Ichoku, O;Gonzalez-Flamenco, G;Park, S;Aljudi, A;DeRyckere, D;Castellino, S;Rafiq, S;Langermann, S;Liu, L;Henry, C;Porter, C;Siglec-15 promotes evasion of adaptive immunity in B-cell acute lymphoblastic leukemiaCancer Research CommunicationsAll samples were acquired after written informed consent was provided. Residual, fresh bone marrow aspirate and peripheral blood samples from pediatric B-ALL patients were analyzed for Sig15 expression by flow cytometry under protocol (IRB#96145). BoFull Paper
2023Kessler, E;Callihan, E;Hu, J;Eule, C;Srivastava, G;Kemme, D;Iruku, P;Rana, V;Moore, J;Schuster, S;Amirault, M;Flaig, T;Lam, E;Data from A Phase I/II Clinical Trial of Pembrolizumab and Cabozantinib in Metastatic Renal Cell CarcinomaAmerican Association for Cancer ResearchPD-L1 testing was performed on archival tumor tissues by Discovery Life Sciences (formerly QualTek Molecular Laboratories) using a previously validated IHC assay for SaveFull Paper
2023Sharma, P;Zhang, X;Ly, K;Kim, J;Wan, Q;Kim, J;Lou, M;Kain, L;Teyton, L;Winau, F;Hyperglycosylation of prosaposin in tumor DCs promotes immune escape in cancerbioRxivDissociated whole tumor samples were purchased from Discovery Life Sciences (USA). Melanoma, myeloid, and CD8 T cells were FACS-sorted as described above. Melanoma cells were ?-irradiated (10,000 rad), and induction of apoptosis wasFull Paper
2023Tierney, B;Foox, J;Ryon, K;Butler, D;Damle, N;Young, B;Mozsary, C;Babler, K;Yin, X;Carattini, Y;Andrews, D;Solle, N;Kumar, N;Shukla, B;Vidovic, D;Currall, B;Williams, S;Schürer, S;Stevenson, M;Amirali, A;Beaver, C;Kobetz, E;Boone, M;Reding, B;Laine, J;Comerford, S;Lamar, W;Tallon, J;Hirschberg, J;Proszynski, J;Sharkey, M;Church, G;Grills, G;Solo-Gabriele, H;Mason, C;Geospatially-resolved public-health surveillance via wastewater sequencingmedRxiv550 HudsonAlpha Discovery (Huntsville, AL) performed all sequencing library preparation on the 551 generated nucleic acids. The nucleic acid samples underwent SaveFull Paper
2023Zhang, X;Meng, F;Lyu, W;He, J;Wei, R;Du, Z;Zhang, C;Guan, Y;Huang, X;Lyu, G;Tian, X;Zhang, L;Tao, W;Histone lactylation antagonizes senescence and skeletal muscle aging via facilitating gene expression reprogrammingbioRxivIMR90 cells were purchased from the American Type Culture Collection (ATCC), HUVECs were purchased from AllCells, and MEF cells were isolated from 12.5-14- day-old embryos of C57BL/6 miceFull Paper
2023Sorensen, JT;Classification of a novel subgroup of shared hepatitis B virus core and e antigen epitopes and their antibody reactivity in clinical immunoassay formatsThesisProtocols for BSL-2 ELISA are summarized in section 5.3.1. HBV positive samples were all purchased from Discovery Life Sciences Biological Sample RepositoryFull Paper
2023Najia, MA;Transposable elements and the regulatory logic of hematopoietic differentiationThesisumbilical cord blood cells (AllCells) were thawed from liquid nitrogen storage via CD34+ umbilical cord blood cells (AllCells) were thawed from liquid nitrogen storage and SaveFull Paper
2023Schejtman Borisonik, A;Lentiviral gene therapy for p47-deficient Chronic Granulomatous diseaseThesisCD34+ cells used in the experiments were frozen vials of previously isolated HD CD34+ cells from a G-CSF mobilised apheresis (ALLCells) using the CliniMACS prodigyFull Paper
2023Kortchak, S;Development of Programmed Cell Death Ligand Derived Peptides for the Treatment of Autoimmune DiseasesThesisCell Culture. Jurkat NFAT-PD-1 reporter cells (BPS Biosciences, San Diego, CA) were maintained in Growth Medium 2A (BPS Biosciences, San Diego, CA) medium. Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells and maintained in RPMIFull Paper
2023Maughon Jr, TS;Metabolic Profiling of Mesenchymal Stromal Cells for Critical Quality Attribute IdentificationThesisat three densities (10,000, 5,000 and 2,000 cells/well) in a 96 well plate and cultured for 24 hours. PBMCs (AllCells, Alameda CA) were thawed in Roswell Park Memorial Institute (RPMI) media (RPMI, 20% FBS, 2mM L-glutamine, 50 U/mL penicillin, 50 ?g/Full Paper
2023Han, L;Dong, L;Leung, K;Zhao, Z;Li, Y;Gao, L;Chen, Z;Xue, J;Qing, Y;Li, W;Pokharel, SP;Gao, M;Chen, M;Shen, C;Tan, B;Small, A;Wang, K;Zhang, Z;Qin, X;Yang, L;Wunderlich, M;Zhang, B;Mulloy, JC;Marcucci, G;Chen, CW;Wei, M;Su, R;Chen, J;Deng, X;METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolismCell stem cell36608679ColonyGEL-Mouse Base Medium Reachbio Cat# 1201 ColonyGEL -Human Base Medium Reachbio Cat# 1101Full Paper
2023Le Floc'h, A;Nagashima, K;Birchard, D;Scott, G;Ben, LH;Ajithdoss, D;Gayvert, K;Romero Hernandez, A;Herbin, O;Tay, A;Farrales, P;Korgaonkar, CK;Pan, H;Shah, S;Kamat, V;Chatterjee, I;Popke, J;Oyejide, A;Lim, WK;Kim, JH;Huang, T;Franklin, M;Olson, W;Norton, T;Perlee, L;Yancopoulos, GD;Murphy, AJ;Sleeman, MA;Orengo, JM;Blocking common γ chain cytokine signaling ameliorates T cell-mediated pathogenesis in disease modelsScience translational medicine36630481To induce GVHD, we injected immunodeficient NSG (Jackson Laboratory, 005557) female mice (8 weeks old) retro-orbitally with 10 7 human PBMCs (ReachBio, 0500-401). Mice were SaveFull Paper
2023Wang, H;Georgakopoulou, A;Zhang, W;Kim, J;Gil, S;Ehrhardt, A;Lieber, A;HDAd6/35++ - A new helper-dependent adenovirus vector platform for in vivo transduction of hematopoietic stem cellsMolecular therapy. Methods & clinical development37081854Lineage-negative (Lin-) cells were isolated by depletion of lineage-committed cells in BM MNCs using the mouse lineage cell depletion kit (Miltenyi Biotec) according to the manufacturer’s instructions. Colony-forming unit assays were performed usingFull Paper
2023Belcher, JD;Nataraja, S;Abdulla, F;Zhang, P;Chen, C;Nguyen, J;Ruan, C;Singh, M;Demes, S;Olson, L;Stickens, D;Stanwix, J;Clarke, E;Huang, Y;Biddle, M;Vercellotti, GM;The BACH1 inhibitor ASP8731 inhibits inflammation and vaso-occlusion and induces fetal hemoglobin in sickle cell diseaseFrontiers in medicine37144034Department of Medicine, Division of Hematology, Oncology and Transplantation, University of Minnesota, Minneapolis, MN, United States.++Mitobridge Inc., Cambridge, MA, United States.++Department of Medicine, Division of Hematology, Oncology and TransFull Paper
2023Wu, T;Pelus, LM;Plett, PA;Sampson, CH;Chua, HL;Fisher, A;Feng, H;Liu, L;Li, H;Ortiz, M;Chittajallu, S;Luo, Q;Bhatwadekar, AD;Meyer, TB;Zhang, X;Zhou, D;Fischer, KD;McKinzie, DL;Miller, SJ;Orschell, CM;Further Characterization of Multi-Organ DEARE and Protection by 16,16 Dimethyl Prostaglandin E2 in a Mouse Model of the Hematopoietic Acute Radiation SyndromeRadiation research37014943Total nucleated BM cells from non-irradiated mice were plated at 2 × 104 per dish in ColonyGEL 1202 Mouse Complete Medium (Reach Bio, Seattle, WA)Full Paper
2023Borot, F;Humbert, O;Newby, GA;Fields, E;Kohli, S;Radtke, S;Laszlo, GS;Mayuranathan, T;Ali, AM;Weiss, MJ;Yen, JS;Walter, RB;Liu, DR;Mukherjee, S;Kiem, HP;Multiplex Base Editing to Protect from CD33-Directed Therapy: Implications for Immune and Gene TherapybioRxiv : the preprint server for biology36865281Cord blood CD34+ 526 cells were 527 additionally cultured with UM171 0.35 nM (Xcessbio). In colony-forming cell assays, 1000-1200 528 sorted cells were seeded into 3.5 ml ColonyGEL 1402 (ReachBio)Full Paper
2023Vujovic, A;de Rooij, L;Chahi, AK;Chen, HT;Yee, BA;Loganathan, SK;Liu, L;Chan, DCH;Tajik, A;Tsao, E;Moreira, S;Joshi, P;Xu, J;Wong, N;Balde, Z;Jahangiri, S;Zandi, S;Aigner, S;Dick, JE;Minden, MD;Schramek, D;Yeo, GW;Hope, KJ;In Vivo Screening Unveils Pervasive RNA-Binding Protein Dependencies in Leukemic Stem Cells and Identifies ELAVL1 as a Therapeutic TargetBlood cancer discovery36763002Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio cat. no. 1102), supplemented with 1.1 μmol/L DHTS (MilliporeSigma; cat. no. D0947-10MG) or equivalent DMSO (FishFull Paper
2023Zhang, Z;Zhou, K;Han, L;Small, A;Xue, J;Huang, H;Weng, H;Su, R;Tan, B;Shen, C;Li, W;Zhao, Z;Qing, Y;Qin, X;Wang, K;Leung, K;Boldin, M;Chen, C;Ann, D;Qian, Z;Deng, X;Chen, J;Chen, Z;RNA m6A reader YTHDF2 facilitates precursor miR-126 maturation to promote acute myeloid leukemia progressionGenes & DiseasesColonyGel 1201-mouse base medium (1201, Reachbio) were used for seeding cells at 20, 000 per 35 mm dish. Murine cytokines and reagents were supplemented as follows: 10 ng/ SaveFull Paper
2023Davies, J;Prieto, EM;Guelcher, S;Characterization of Macrophage Behavior in the Human Immunoresponse and Bone Remodelingcdn.vanderbilt.eduWe cultured primary human monocytes (Reachbio, Seattle, WA) in Corning T25 flasks with 10 mL of modified Dulbecco’s modified Eagle medium (DMEM) media and split them every 9 days. We changed media on Day 1, 5, and 7, according to Table 1. PenicillinFull Paper
2023Vujovic, A;Investigating RNA-Binding Protein Dependencies in Hematopoietic and Acute Myeloid Leukemic Stem CellsThesisThawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio Cat# 1102), supplemented with 1.1μM DHTS (MilliporeSigma Cat# D0947-10MG) or equivalent DMSO (Fisher ScientificFull Paper
2022Somarathna, M;Hwang, PT;Millican, RC;Alexander, GC;Isayeva-Waldrop, T;Sherwood, JA;Brott, BC;Falzon, I;Northrup, H;Shiu, YT;Stubben, CJ;Totenhagen, J;Jun, HW;Lee, T;Nitric oxide releasing nanomatrix gel treatment inhibits venous intimal hyperplasia and improves vascular remodeling in a rodent arteriovenous fistulaBiomaterials34836683RNA isolation and sequencing were performed by Discovery Life Sciences (Huntsville, AL). Total RNA was isolated using standard Discovery Life Sciences protocols. All samples underwent RNA quality control assessment using a Fragment Analyzer (AgilenFull Paper
2022Choi, DY;Park, JN;Paek, SH;Choi, SC;Paek, SH;Detecting early-stage malignant melanoma using a calcium switch-enriched exosome subpopulation containing tumor markers as a sampleBiosensors & bioelectronics34847362Clinical samples of human sera from healthy donors and malignant melanoma patients were obtained from Discovery Life Sciences (DLS; Los Osos, CA, USA). Other materials are listed and described about the sources in details in Supplementary InformatiFull Paper
2022Bormann Chung, C;Lee, J;Barritault, M;Bringuier, PP;Xu, Z;Huang, WY;Beharry, A;Castillo, J;Christiansen, J;Lin, JC;Sheffield, BS;Evaluating Targeted Next-Generation Sequencing Assays and Reference Materials for NTRK Fusion DetectionThe Journal of molecular diagnostics : JMD34656759The 30 FFPE NTRK fusion-negative tumor samples (confirmed as NTRK fusion negative by an NGS laboratory-developed test) tested in the specificity analyses for AFL, TSO500, and OFA were purchased from Indivumed GmbH (Hamburg, Germany) and Discovery LifFull Paper
2022Freeman, J;Conklin, J;Standardization of two SARS-CoV-2 serology assays to the WHO 20/136 human standard reference materialJournal of virological methods34915088WHO 20/136 is standard reference material for SARS-CoV-2 serology assays. Standardization of serology assays that target the same antigen and class of immunoglobulin will enable comparison of results between studies that use various lab-developed aFull Paper
2022Ganan-Gomez, I;Yang, H;Ma, F;Montalban-Bravo, G;Thongon, N;Marchica, V;Richard-Carpentier, G;Chien, K;Manyam, G;Wang, F;Alfonso, A;Chen, S;Class, C;Kanagal-Shamanna, R;Ingram, JP;Ogoti, Y;Rose, A;Loghavi, S;Lockyer, P;Cambo, B;Muftuoglu, M;Schneider, S;Adema, V;McLellan, M;Garza, J;Marchesini, M;Giuliani, N;Pellegrini, M;Wang, J;Walker, J;Li, Z;Takahashi, K;Leverson, JD;Bueso-Ramos, C;Andreeff, M;Clise-Dwyer, K;Garcia-Manero, G;Colla, S;Stem cell architecture drives myelodysplastic syndrome progression and predicts response to venetoclax-based therapyNature medicine35241842simultaneously received the cytidine deaminase inhibitor E7727 (cedazuridine) to increase HMA bioavailability. BM samples from 21 HDs (median age, 53 years; range, 49-66 years) were obtained from AllCells or from MD Anderson’s Department of Stem CelFull Paper
2022Lebedin, M;Foglierini, M;Khorkova, S;Vázquez García, C;Ratswohl, C;Davydov, AN;Turchaninova, MA;Daubenberger, C;Chudakov, DM;Lanzavecchia, A;de la Rosa, K;Different classes of genomic inserts contribute to human antibody diversityProceedings of the National Academy of Sciences of the United States of America3603735342), and ethical review boards at Ifakara Health Institute and the University of Lausanne, and all volunteers provided informed consent before blood donation. Bone marrow specimens were obtained from AllCells. Extraction of Human PBMCs. Blood sampleFull Paper
2022Simmons, SK;Lithwick-Yanai, G;Adiconis, X;Oberstrass, F;Iremadze, N;Geiger-Schuller, K;Thakore, PI;Frangieh, CJ;Barad, O;Almogy, G;Rozenblatt-Rosen, O;Regev, A;Lipson, D;Levin, JZ;Mostly natural sequencing-by-synthesis for scRNA-seq using Ultima sequencingNature biotechnology36109685We purchased nine cryopreserved human PMBC samples (AllCells). We thawed PBMC vials in a 37 °C water bath for ~2 min. A quick counting revealed high viability (90%) in all samples. We added 1 ml RPMI1640 (Thermo Fisher Scientific, 11875093) with 10%Full Paper
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2022Omer-Javed, A;Pedrazzani, G;Albano, L;Ghaus, S;Latroche, C;Manzi, M;Ferrari, S;Fiumara, M;Jacob, A;Vavassori, V;Nonis, A;Canarutto, D;Naldini, L;Mobilization-based chemotherapy-free engraftment of gene-edited human hematopoietic stem cellsCell35617958V4XP-3032 TaqMan Gene Expression Assay ThermoFisher Scientifi Cat# 4331182 ------------------------- Experimental models: Cell lines ------------------------- Human: G-CSF mPB CD34+ HSPCs AllCells N/A Human: G-CSF/Mozobil mPB CD34+ HSFull Paper
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2022Dey, M;Kim, M;Dogan, M;Nagamine, M;Kozhaya, L;Celik, N;Unutmaz, D;Ozbolat, I;Chemotherapeutics and CAR‐T Cell‐Based Immunotherapeutics Screening on a 3D Bioprinted Vascularized Breast Tumor ModelAdvanced Functional MaterialsLeukopaks from healthy adults were purchased from ALLCells (Alameda, CA) in deidentified form and processed to isolate Peripheral Blood Mononuclear Cells (PBMCs) using Ficoll-paque plus (GE Healthcare). CD8+ T cells were purified from PBMCs using DynFull Paper
2022Elagib, KE;Brock, A;Clementelli, CM;Mosoyan, G;Delehanty, LL;Sahu, RK;Pacheco-Benichou, A;Fruit, C;Besson, T;Morris, SW;Eto, K;Jobaliya, C;French, DL;Gadue, P;Singh, S;Shi, X;Qin, F;Cornelison, R;Li, H;Iancu-Rubin, C;Goldfarb, AN;Relieving DYRK1A repression of MKL1 confers an adult-like phenotype to human infantile megakaryocytesThe Journal of clinical investigation35925681alculator/hypergeometric.aspx). A P value less than 0.05 was considered significant. Study approval. CD34+ cells purchased from the Fred Hutchinson Cancer Research Center (Seattle, Washington, USA), AllCells (Alameda, California, USA), and StemCellFull Paper
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2022Sandberg, ML;Wang, X;Martin, AD;Nampe, DP;Gabrelow, GB;Li, CZ;McElvain, ME;Lee, WH;Shafaattalab, S;Martire, S;Fisher, FA;Ando, Y;Liu, E;Ju, D;Wong, LM;Xu, H;Kamb, A;A carcinoembryonic antigen-specific cell therapy selectively targets tumor cells with HLA loss of heterozygosity in vitro and in vivoScience translational medicine35235342Informed consent for primary T cells and donor collection protocols were approved by an Institutional Review Board at AllCells or HemaCare. Peripheral blood mononuclear cells (PBMCs) were purified from Leukopaks purchased from AllCells or HemaCare, aFull Paper
2022Uehara, K;Harumoto, T;Makino, A;Koda, Y;Iwano, J;Suzuki, Y;Tanigawa, M;Iwai, H;Asano, K;Kurihara, K;Hamaguchi, A;Kodaira, H;Atsumi, T;Yamada, Y;Tomizuka, K;Targeted delivery to macrophages and dendritic cells by chemically modified mannose ligand-conjugated siRNANucleic acids research35524566are). Preparation of human monocyte-derived macrophages and DCs Two types of human monocyte-derived macrophages (h-Mo-Mφs) were generated as described in Supplementary Figure S1A. CD14+ monocytes (AllCells, Alameda, CA, USA) were cultured (37°C, 5%Full Paper
2022Pennycuick, A;Selway, H;Lam, J;Khan, K;331P Predicting outcomes following colorectal cancer resection: Using real-world data to empower adjuvant treatment decision makingAnnals of Oncology: Financial Interests, Personal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. A. Vivancos: Financial Interests, PersoFull Paper
2022Glaser, M;von Levetzow, C;Michels, S;Nogova, L;Katzenmeier, M;Wömpner, C;Schmitz, J;Bitter, E;Terjung, I;Passmann, E;Schaufler, D;Eisert, A;Fischer, R;Riedel, R;Weber, J;Hahne, S;Merkelbach-Bruse, S;Büttner, R;Wolf, J;Scheffler, M;1729P The biological effect of small-scale ROS1 aberrations: An in silico analysisAnnals of OncologyMS. R. Riedel: Financial Interests, Personal, Funding: Boehringer Ingelheim, Loxo Oncology, Novartis, Lilly. J. Weber: Financial Interests, Personal, Advisory Board: Lilly. R. Büttner: Financial Interests, Personal, Ownership Interest: Targos MoleculFull Paper
2022Poole, A;Karuppiah, V;Hartt, A;Haidar, JN;Moureau, S;Dobrzycki, T;Hayes, C;Rowley, C;Dias, J;Harper, S;Barnbrook, K;Hock, M;Coles, C;Yang, W;Aleksic, M;Lin, AB;Robinson, R;Dukes, JD;Liddy, N;Van der Kamp, M;Plowman, GD;Vuidepot, A;Cole, DK;Whale, AD;Chillakuri, C;Therapeutic high affinity T cell receptor targeting a KRASG12D cancer neoantigenNature communications36088370ersberg, Germany). PBMCs were either purchased cryo-frozen from commercial suppliers (Cellular Technology Limited (CTL), OH & Tissue Solutions, Glasgow, UK) or prepared from healthy donor leukopaks (ALLCELLS, CA) by Ficoll density gradient centrifugFull Paper
2022Sano, Y;Azuma, Y;Tsunenari, T;Kayukawa, Y;Shinozuka, J;Fujii, E;Amano, J;Nishito, Y;Maruyama, T;Kinoshita, Y;Sakamoto, Y;Yoshida, A;Miyazaki, Y;Sato, Y;Teramoto-Seida, C;Ishiguro, T;Tanaka, T;Kitazawa, T;Endo, M;Combination of T cell-redirecting bispecific antibody ERY974 and chemotherapy reciprocally enhances efficacy against non-inflamed tumoursNature communications36071036(Tokyo, Japan). After an acclimatisation of 3 weeks, mice were irradiated (2.5 Gy; MBR-1520R-3; Hitachi Power Solutions Co. Ltd., Ibaraki, Japan) 1 day before transplantation of human CD34+ cells (AllCells, Alameda, CA, USA) via intravenous injectionFull Paper
2022Badrani, JH;Strohm, AN;Lacasa, L;Civello, B;Cavagnero, K;Haung, YA;Amadeo, M;Naji, LH;Lund, SJ;Leng, A;Kim, H;Baum, RE;Khorram, N;Mondal, M;Seumois, G;Pilotte, J;Vanderklish, PW;McGee, HM;Doherty, TA;RNA-binding protein RBM3 intrinsically suppresses lung innate lymphoid cell activation and inflammation partially through CysLT1RNature communications35908044&D Systems, Minneapolis, MN) in T cell media. Post-stimulation supernatant was collected and stored at −80 C for ELISA analysis. Human PBMCs were isolated from commercially purchased leukopacks (Allcells, Alameda, CA, USA). Human peripheral blood ILFull Paper
2022Li, F;Zhang, H;Wang, W;Yang, P;Huang, Y;Zhang, J;Yan, Y;Wang, Y;Ding, X;Liang, J;Qi, X;Li, M;Han, P;Zhang, X;Wang, X;Cao, J;Fu, YX;Yang, X;T cell receptor β-chain-targeting chimeric antigen receptor T cells against T cell malignanciesNature communications35882880ded by Dr. Justin Kline (University of Chicago). B16-OVA cells were kindly provided by Dr. Hans Schreiber (University of Chicago). Human peripheral blood mononuclear cells (PBMCs) were purchased from Allcells Biotechnology, Limited (Shanghai, China)Full Paper
2022Jo, S;Das, S;Williams, A;Chretien, AS;Pagliardini, T;Le Roy, A;Fernandez, JP;Le Clerre, D;Jahangiri, B;Chion-Sotinel, I;Rozlan, S;Dessez, E;Gouble, A;Dusséaux, M;Galetto, R;Duclert, A;Marcenaro, E;Devillier, R;Olive, D;Duchateau, P;Poirot, L;Valton, J;Endowing universal CAR T-cell with immune-evasive properties using TALEN-gene editingNature communications35773273cell products and to allow their large-scale utilization against multiple malignancies for the benefit of a broader range of patients. Methods Materials Cryopreserved human PBMCs were acquired from ALLCELLS (cat# PB006F). PBMCs were cultured in CTSFull Paper
2022Cai, T;Gouble, A;Black, KL;Skwarska, A;Naqvi, AS;Taylor, D;Zhao, M;Yuan, Q;Sugita, M;Zhang, Q;Galetto, R;Filipe, S;Cavazos, A;Han, L;Kuruvilla, V;Ma, H;Weng, C;Liu, CG;Liu, X;Konoplev, S;Gu, J;Tang, G;Su, X;Al-Atrash, G;Ciurea, S;Neelapu, SS;Lane, AA;Kantarjian, H;Guzman, ML;Pemmaraju, N;Smith, J;Thomas-Tikhonenko, A;Konopleva, M;Targeting CD123 in blastic plasmacytoid dendritic cell neoplasm using allogeneic anti-CD123 CAR T cellsNature communications35484100The production of R&D grade batches of UCART123 was done using commercially available NHPBMC cells ordered from ALLCELLS or HEMACARE. These are normal human SaveFull Paper
2022Salas, LA;Zhang, Z;Koestler, DC;Butler, RA;Hansen, HM;Molinaro, AM;Wiencke, JK;Kelsey, KT;Christensen, BC;Enhanced cell deconvolution of peripheral blood using DNA methylation for high-resolution immune profilingNature communications35140201lymphocytes memory (CD4mem), T regulatory cells (Treg), T-cytotoxic lymphocytes naïve (CD8nv), T-cytotoxic lymphocytes memory (CD8mem), and natural killer lymphocytes (NK) cells] were purchased from AllCells corporation (Alameda, CA, USA), StemExpreFull Paper
2022Wiesweg, M;Hense, J;Darwiche, K;Michels, S;Hautzel, H;Kobe, C;Metzenmacher, M;Herold, T;Zaun, G;Laue, K;Drzezga, A;Schildhaus, H;Wolf, J;Herrmann, K;Schuler, M;1171P A phase II theranostic study with osimertinib in patients with EGFR-mutated non-small cell lung cancer (NSCLC) progressing on EGFR tyrosine kinase inhibitors (TKI) and undetectable EGFR T790M (THEROS)Annals of Oncologyovartis Oncology; Financial Interests, Personal, Full or part-time Employment: Targos Molecular Pathology, Inc. J. Wolf: Financial Interests, Personal, Advisory Board: Amgen, AstraZeneca, Bayer, Blueprint, BMS, Boehringer Ingelheim, Chugai, Daiichi SFull Paper
2022Chansaenroj, A;Adine, C;Charoenlappanit, S;Roytrakul, S;Sariya, L;Osathanon, T;Rungarunlert, S;Urkasemsin, G;Chaisuparat, R;Yodmuang, S;Souza, GR;Ferreira, JN;Magnetic bioassembly platforms towards the generation of extracellular vesicles from human salivary gland functional organoids for epithelial repairBioactive materials35387159d EV; and (3) to identify novel signaling cues in EV that may optimize organoid development towards epithelial SG repair. 2 Materials and methods 2.1 Adult stem cell culture hDPSC were obtained from AllCells (Alameda, CA, USA) and cultured in DulbecFull Paper
2022Kavanagh, ME;Horning, BD;Khattri, R;Roy, N;Lu, JP;Whitby, LR;Ye, E;Brannon, JC;Parker, A;Chick, JM;Eissler, CL;Wong, AJ;Rodriguez, JL;Rodiles, S;Masuda, K;Teijaro, JR;Simon, GM;Patricelli, MP;Cravatt, BF;Selective inhibitors of JAK1 targeting an isoform-restricted allosteric cysteineNature chemical biology36097295In vitro TE50 values for JAK1_C817 and TYK2_C838 were obtained by treating 500 µg cell lysate generated from primary human PBMCs (AllCells), Jurkat T cells (ATCC, #TIB-152), or MDA-MB-468 breast cancer cells (ATCC, #HTB-132), with DMSO or compound foFull Paper
2022Lam, J;Lee, B;Yu, J;Kwee, BJ;Kim, Y;Kim, J;Choi, Y;Yoon, JS;Kim, Y;Baek, K;Jeon, NL;Sung, KE;A microphysiological system-based potency bioassay for the functional quality assessment of mesenchymal stromal cells targeting vasculogenesisBiomaterials36201944Human bone marrow-derived MSCs were obtained from eight different donors purchased from RoosterBio (RB9, RB12, RB14, RB16; Frederick, MD) at passage 1 (P1), Lonza (8F560, 167,696; Walkersville, MD) at passage 2 (P2), and AllCells (PCBM1632, PCBM1641;Full Paper
2022Han, C;Khodadadi-Jamayran, A;Lorch, AH;Jin, Q;Serafin, V;Zhu, P;Politanska, Y;Sun, L;Gutierrez-Diaz, BT;Pryzhkova, MV;Abdala-Valencia, H;Bartom, ET;Buldini, B;Basso, G;Velu, SE;Sarma, K;Mattamana, BB;Cho, BK;Obeng, RC;Goo, YA;Jordan, PW;Tsirigos, A;Zhou, Y;Ntziachristos, P;SF3B1 homeostasis is critical for survival and therapeutic response in T cell leukemiaScience advances35061527ing PCR to detect the TCRb-NOTCH1 translocation (TCRBJ2S4CUTLL1F: 5′-GGACCCGGCTCTCAGTGCT-3′, NOTCH1CUTTL1R: 5′-TCCCGCCCTCCAAAATAAGG-3′). Human CD3+, CD8+, and CD4+ T cells were purchased from AllCells.com (Alameda, CA) or from Astarte Biologics (BothFull Paper
2022Pan, Z;Chen, J;Xiao, X;Xie, Y;Jiang, H;Zhang, B;Lu, H;Yuan, Y;Han, L;Zhou, Y;Zong, H;Wang, L;Sun, R;Zhu, J;Characterization of a novel bispecific antibody targeting tissue factor-positive tumors with T cell engagementActa pharmaceutica Sinica. B35847491for the T cell activation test were bought from AllCells (Shanghai, China). PBMC used in other experiments were isolated from leukocyte concentrate (AllCells) using Ficoll-Paque PLUS ( Save;nded conditions and used within 2 months after resuscitatioFull Paper
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2022Peng, S;Hu, P;Xiao, YT;Lu, W;Guo, D;Hu, S;Xie, J;Wang, M;Yu, W;Yang, J;Chen, H;Zhang, X;Zhu, Y;Wang, Y;Yang, Y;Zhu, G;Chen, S;Wang, J;Zhang, B;Chen, W;Wu, H;Sun, Z;Ding, T;Zhang, H;Yi, Z;Liu, M;Ren, S;Single-Cell Analysis Reveals EP4 as a Target for Restoring T-Cell Infiltration and Sensitizing Prostate Cancer to ImmunotherapyClinical cancer research : an official journal of the American Association for Cancer Research34740924Purpose: Immunotherapies targeting immune checkpoint molecules have shown promising treatment for a subset of cancers; however, many "cold" tumors, such as prostate cancer, SaveFull Paper
2022Siegel, RJ;Singh, AK;Panipinto, PM;Shaikh, FS;Vinh, J;Han, SU;Kenney, HM;Schwarz, EM;Crowson, CS;Khuder, SA;Khuder, BS;Fox, DA;Ahmed, S;Extracellular sulfatase-2 is overexpressed in rheumatoid arthritis and mediates the TNF-?-induced inflammatory activation of synovial fibroblastsCellular & molecular immunology36068294Human serum samples were purchased from commercial vendors (Discovery Life Sciences, Newtown, PA; Innovative Research, Novi, MI; or Sanguine Biosciences, Los Angeles, CA) or generously shared by Dr. Cynthia Crowson (Mayo Clinic, Rochester, MN)Full Paper
2022Gyurdieva, A;Zajic, S;Chang, YF;Houseman, EA;Zhong, S;Kim, J;Nathenson, M;Faitg, T;Woessner, M;Turner, DC;Hasan, AN;Glod, J;Kaplan, RN;D'Angelo, SP;Araujo, DM;Chow, WA;Druta, M;Demetri, GD;Van Tine, BA;Grupp, SA;Fine, GD;Eleftheriadou, I;Biomarker correlates with response to NY-ESO-1 TCR T cells in patients with synovial sarcomaNature communications36075914s were digitally obtained using CaseViewer (3D Histech, Budapest, Hungary). Protein expression by IHC NY-ESO-1 staining was performed using the E978 clone (Sigma, Catalog #: N2038, at 1??g/mL) at QualTek Laboratory (Goleta, CA; now part of DiscoveryFull Paper
2022Ni, Z;Sun, P;Zheng, J;Wu, M;Yang, C;Cheng, M;Yin, M;Cui, C;Wang, G;Yuan, L;Gao, Q;Li, Y;JNK Signaling Promotes Bladder Cancer Immune Escape by Regulating METTL3-Mediated m6A Modification of PD-L1 mRNACancer research35502544CD8+ T cells (catalog no. PB009-3F-C) were obtained from AllCells (Shanghai) and cultured in ImmunoCult-XF T Cell Expansion Medium (STEMCELL Technologies, catalog no. 10981). Seventy-two hours after activation with ImmunoCult Human CD3/CD28/CD2 T CelFull Paper
2022Ge, X;Yost, SE;Lee, JS;Frankel, PH;Ruel, C;Cui, Y;Murga, M;Tang, A;Martinez, N;Chung, S;Yeon, C;Stewart, D;Li, D;Rajurkar, S;Somlo, G;Mortimer, J;Waisman, J;Yuan, Y;Phase II Study Combining Pembrolizumab with Aromatase Inhibitor in Patients with Metastatic Hormone Receptor Positive Breast CancerCancers36077811mal TILs were measured by hematoxylin and eosin (H&E) staining of formalin-fixed paraffin-embedded (FFPE) tumor biopsies according to the International TIL Work Group guideline [16]. PD-L1 analysis: QualTek Molecular Laboratory (Goleta, CA, USA) perFull Paper
2022Shirk, B;Shirk, P;Furlong, R;Scully, E;Wu, K;Siegfried, B;Gene Editing of the ABC Transporter/White Locus Using Crispr/Cas9-Mediated Mutagenesis in the Indian Meal MothSSRN Electronic Journaly and sequencing, and Kris Hartzer for laboratory assistance. Library 497 preparation and sequencing were performed at HudsonAlpha Genomic Services Lab 498 (Huntsville, AL). We also thank Karen Garren for her work to isolate the P. interpunctella 499Full Paper
2022Kanter, J;Thompson, AA;Pierciey, FJ;Hsieh, M;Uchida, N;Leboulch, P;Schmidt, M;Bonner, M;Guo, R;Miller, A;Ribeil, JA;Davidson, D;Asmal, M;Walters, MC;Tisdale, JF;Lovo-cel gene therapy for sickle cell disease: Treatment process evolution and outcomes in the initial groups of the HGB-206 studyAmerican journal of hematology36161320MCW served as medical director for AllCells and as a consultant for AllCells, Ensoma, Vertex, and BioLabs. The remaining authors declare no competing financial interests. SaveFull Paper
2022Wei, Y;Kanagal-Shamanna, R;Zheng, H;Bao, N;Lockyer, PP;Class, CA;Darbaniyan, F;Lu, Y;Lin, K;Yang, H;Montalban-Bravo, G;Ganan-Gomez, I;Soltysiak, KA;Do, KA;Colla, S;Garcia-Manero, G;Cooperation between KDM6B overexpression and TET2 deficiency in the pathogenesis of chronic myelomonocytic leukemiaLeukemia35697791Materials and methods Human samples BM specimens were obtained from the MD Anderson Cancer Center tissue bank following institutional guidelines. BM samples from healthy individuals were obtained from AllCells (Emeryville, CA)Full Paper
2022Lu, M;Xia, L;Elmansy, N;Clementelli, C;Tremblay, D;Hoffman, R;Combined Drug Targeting of p53-dependent and -independent Pathways Depletes Myelofibrosis Hematopoietic Stem/Progenitor CellsLeukemia34642468met the World Health Organization criteria for the diagnosis of MF [41, 42]. Patient characteristics are provided in Supplemental Table 1. Normal donor (ND) human bone marrow (BM) was purchased from AllCells (Emeryville, CA). The experiments in whichFull Paper
2022Bae, J;Accardi, F;Hideshima, T;Tai, YT;Prabhala, R;Shambley, A;Wen, K;Rowell, S;Richardson, PG;Munshi, NC;Anderson, KC;Targeting LAG3/GAL-3 to overcome immunosuppression and enhance anti-tumor immune responses in multiple myelomaLeukemia34290359roval by the Institutional Review Board at Dana-Farber Cancer Institute (Boston, MA). In addition, healthy individuals BM [N = 5] or leukapheresis [N = 12] products were purchased from either AllCells (Alameda, CA) or the Blood Donor Center at BostonFull Paper
2022Huang, G;Cheng, Z;Hildebrand, A;Wang, C;Cimini, M;Roy, R;Lucchese, AM;Benedict, C;Mallaredy, V;Magadum, A;Joladarashi, D;Thej, C;Gonzalez, C;Trungcao, M;Garikipati, VNS;Elrod, JW;Koch, WJ;Kishore, R;Diabetes impairs cardioprotective function of endothelial progenitor cell-derived extracellular vesicles via H3K9Ac inhibitionTheranostics35673580days, db/+ EPCs were cultured at 37°C, 5% CO2 atmosphere. Male mice were used to avoid the reproductive hormone effect in female mice. Human CD34+ Hematopoietic stem cells (HSC) were purchased from AllCells, LLC and were cultured in a human hematopoiFull Paper
2022Shen, Y;Eng, JS;Fajardo, F;Liang, L;Li, C;Collins, P;Tedesco, D;Nolan-Stevaux, O;Cancer cell-intrinsic resistance to BiTE therapy is mediated by loss of CD58 costimulation and modulation of the extrinsic apoptotic pathwayJournal for immunotherapy of cancer35296559Target cells were cocultured with freshly thawed human CD3 + T cells (ALLCELLS) and increasing concentrations of TCEs. Assay conditions were effector-to-target ratio (E:T)=2:1 and SaveFull Paper
2022Froning, KJ;Sereno, A;Huang, F;Demarest, SJ;Generalizable design parameters for soluble T cell receptor-based T cell engagersJournal for immunotherapy of cancer35260435ously11 with minor modifications throughout the protocol. Saos-2 and HCT-116 cells were cultured as described earlier. Primary naïve T cells were from StemExpress (cat#PB03020C, Donor#D001003581) or AllCells (cat# PB009-1F, Lt# 3009286). For the assaFull Paper
2022Wu, ZH;Li, N;Mei, XF;Chen, J;Wang, XZ;Guo, TT;Chen, G;Nie, L;Chen, Y;Jiang, MZ;Wang, JT;Wang, HB;Preclinical characterization of the novel anti-SIRPα antibody BR105 that targets the myeloid immune checkpointJournal for immunotherapy of cancer35256517Allogeneic PBMCs (AllCells) from different donors were added at a dendritic cell:PBMCs ratio of 1:20. Indicated mAbs were added at a concentration of 10 µg/mL immediately, and the SaveFull Paper
2022Lee, KM;Lin, CC;Servetto, A;Bae, J;Kandagatla, V;Ye, D;Kim, G;Sudhan, DR;Mendiratta, S;González Ericsson, PI;Balko, JM;Lee, J;Barnes, S;Malladi, VS;Tabrizi, S;Reddy, SM;Yum, S;Chang, CW;Hutchinson, KE;Yost, SE;Yuan, Y;Chen, ZJ;Fu, YX;Hanker, AB;Arteaga, CL;Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast CancerCancer immunology research35561311After a week, 1 A 107 human PBMCs (hPBMC; ALLCELLS) were injected intravenously. Two weeks later, SUM159PT tumors were harvested for analysis of tumor-infiltrating immune SaveFull Paper
2022Li, W;Yang, S;Xu, P;Zhang, D;Tong, Y;Chen, L;Jia, B;Li, A;Lian, C;Ru, D;Zhang, B;Liu, M;Chen, C;Fu, W;Yuan, S;Gu, C;Wang, L;Li, W;Liang, Y;Yang, Z;Ren, X;Wang, S;Zhang, X;Song, Y;Xie, Y;Lu, H;Xu, J;Wang, H;Yu, W;SARS-CoV-2 RNA elements share human sequence identity and upregulate hyaluronan via NamiRNA-enhancer networkEBioMedicine35124429Human umbilical vein endothelial cells (HUVEC) were cultured in the commercial culture medium (AllCells, Cat# H-004). VERO C1008 (E6) (Cat# GNO17) and its culture medium MEM Save;ed with 10% FBS (Gibco, Cat# 10270-106) and 1% Penicillin-StreptomyciFull Paper
2022Zhang, C;Chen, Z;Liu, J;Wu, M;Yang, J;Zhu, Y;Lu, W;Ruan, C;3D-printed pre-tapped-hole scaffolds facilitate one-step surgery of predictable alveolar bone augmentation and simultaneous dental implantationComposites Part B: EngineeringThe human bone marrow mesenchymal stem cells (hBMSCs, obtained from Allcells, Silicon Valley, CA) were cultured in α-MEM (Gibco, USA) supplemented with 1% penicillin-streptomycin (Gibco, USA) and 10% fetal bovine serum (FBS) (Gibco, USA) in an atmospFull Paper
2022Petaroudi, M;Rodrigo-Navarro, A;Dobre, O;Dalby, MJ;Salmeron-Sanchez, M;Living Biomaterials to Engineer Hematopoietic Stem Cell NichesAdvanced healthcare materials35933595Fresh, cryopreserved bone marrow CD34+ cells isolated from the posterior iliac crest of healthy and consenting donors were purchased from AllCells. All cultures containing CD34+ SaveFull Paper
2022Suyama, T;Takemoto, Y;Miyauchi, H;Kato, Y;Matsuzaki, Y;Kato, R;Morphology-based noninvasive early prediction of serial-passage potency enhances the selection of clone-derived high-potency cell bank from mesenchymal stem cellsInflammation and regeneration36182958Bone marrow mononuclear cells were prepared from bone marrow aspirate (AllCells, Alameda, USA) collected from healthy donors using density gradient centrifugation with Ficoll (GE Save CachedFull Paper
2022Wang, J;Jiang, J;Yang, X;Zhou, G;Wang, L;Xiao, B;Tethering Piezo channels to the actin cytoskeleton for mechanogating via the cadherin-β-catenin mechanotransduction complexCell reports35139384Experimental models: Cell lines HEK293T Xiao et al., 2011 N/A MDCK Xu Tan Lab in Tsinghua University N/A HUVEC Purchased from Allcells N/AFull Paper
2022Sun, Y;Wang, T;Lv, Y;Li, J;Jiang, X;Jiang, J;Zhang, D;Bian, W;Zhang, C;MALAT1 promotes platelet activity and thrombus formation through PI3k/Akt/GSK-3β signalling pathwayStroke and vascular neurology36241224Allcells Biotechnology (Shanghai, China) was the supplier of the Cord Blood-Stem/Progenitor (CD34+) cells that were used in this study. Serum-free expansion medium (StemCell Technologies, Canada) was used in the cultivation of CD34+ cells. To facilitFull Paper
2022Manshouri, T;Veletic, I;Li, P;Yin, CC;Post, SM;Verstovsek, S;Estrov, Z;GLI1 activates pro-fibrotic pathways in myelofibrosis fibrocytesCell death & disease35595725ld Health Organization revised criteria [1]. Control BM biopsies were obtained from morphologically normal BM donors and control BM aspirates were obtained from commercially available healthy donors (AllCells, Alameda, CA, USA). The patients’ and conFull Paper
2022Zhang, J;Liu, F;Yang, Y;Yu, N;Weng, X;Yang, Y;Gong, Z;Huang, S;Gan, L;Sun, S;Zhang, X;Gong, Y;Liu, Y;Guo, W;Integrated DNA and RNA sequencing reveals early drivers involved in metastasis of gastric cancerCell death & disease35449126ze the results using the positive control contained in the different membranes. T-cell amplification and tumor-reactive T cell killing assay Peripheral blood mononuclear cells (PBMCs) purchased from AllCells (China) were activated with anti-CD3 antiFull Paper
2022Liu, P;Zhang, Q;Mi, J;Wang, S;Xu, Q;Zhuang, D;Chen, W;Liu, C;Zhang, L;Guo, J;Wu, X;Exosomes derived from stem cells of human deciduous exfoliated teeth inhibit angiogenesis in vivo and in vitro via the transfer of miR-100-5p and miR-1246Stem cell research & therapy35241153Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Cat. H-001F, AllCells) and were cultured in DMEM (Cat. SH30021.01, Hyclone) with 4.5 g/L D-Glucose SaveFull Paper
2022McLemore, AF;Hou, HA;Meyer, BS;Lam, NB;Ward, GA;Aldrich, AL;Rodrigues, MA;Vedder, A;Zhang, L;Padron, E;Vincelette, ND;Sallman, DA;Abdel-Wahab, O;List, AF;McGraw, KL;Somatic gene mutations expose cytoplasmic DNA to co-opt the cGAS/STING/NLRP3 axis in myelodysplastic syndromesJCI insight35788117using Ficoll-Paque (GE Healthcare) from patients with MDS and healthy donors who provided informed consent at the Moffitt Cancer Center or NTUH or were purchased from AllCells. Save CachedFull Paper
2022Xie, J;Gui, X;Deng, M;Chen, H;Chen, Y;Liu, X;Ku, Z;Tan, L;Huang, R;He, Y;Zhang, B;Lewis, C;Chen, K;Xu, L;Xu, J;Huang, T;Liao, XC;Zhang, N;An, Z;Zhang, CC;Blocking LAIR1 signaling in immune cells inhibits tumor developmentFrontiers in immunology36211388Human allogeneic pan T cells (PB009-1F, Allcells) were stained with Carboxy fluorescein succinimidyl ester (CFSE) (C34554, Thermo Fisher Scientific), and mixed with 3 x10 5 mature SaveFull Paper
2022Hu, JF;Wang, ZW;Liao, CY;Chen, ZW;Kang, FP;Lin, CF;Lin, TS;Huang, L;Tian, YF;Chen, S;Induced expression of CCL19 promotes the anti-tumor ability of CAR-T cells by increasing their infiltration abilityFrontiers in immunology35990619(CRL-1682, ATCC) cells were obtained from ATCC and cultured in RPMI-1640 medium (cat. no. 11875093; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS. Human T cells were obtained from Allcells (cat. no. PB009-CD3-F). Both of the cellsFull Paper
2022Bjornson-Hooper, ZB;Fragiadakis, GK;Spitzer, MH;Chen, H;Madhireddy, D;Hu, K;Lundsten, K;McIlwain, DR;Nolan, GP;A Comprehensive Atlas of Immunological Differences Between Humans, Mice, and Non-Human PrimatesFrontiers in immunology35359965apeutics between humans and model organisms will greatly improve the evaluation of therapeutic candidates. Materials and Methods Blood Venous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human subFull Paper
2022Peng, H;Nerreter, T;Mestermann, K;Wachter, J;Chang, J;Hudecek, M;Rader, C;ROR1-targeting switchable CAR-T cells for cancer therapyOncogene35859167Pan-T cells were isolated from fresh PBMC of healthy donors (Allcells) by negative selection (Pan T Cell Isolation Kit; Miltenyi Biotec), activated using Dynabeads Human T-Activator SaveFull Paper
2022Zhang, M;Zhu, Y;Zhu, J;Xie, Y;Wu, R;Zhong, J;Qiu, Z;Jiang, L;circ_0086296 induced atherosclerotic lesions via the IFIT1/STAT1 feedback loop by sponging miR-576-3pCellular & molecular biology letters36138395HUVECs were obtained from AllCells (Shanghai, China) as previously reported [7, 33]. HUVECs were seeded in EC complete growth medium at 37 C. Then, 100 μg/mL ox-LDL was Save CachedFull Paper
2022Abdul-Aziz, A;Weigel, C;Kovacs, A;Wu, Y;Byrd, J;Hertlein, E;Oakes, C;P381: DNA METHYLATION PROFILING OF MESENCHYMAL STROMAL CELLS ISOLATED FROM FEMURAL HEAD BONE MARROW VERSUS BONE MARROW ASPIRATES: RELEVANCE FOR AML STUDY BASED CONTROLSHemaSphereAspirate-derived BMSCs (n= 7) were expanded from bone marrow mononuclear cells obtained from a commercial source (AllCells). For genome-wide DNA methylation analyses, the SaveFull Paper
2022Owens, TD;Brameld, KA;Verner, EJ;Ton, T;Li, X;Zhu, J;Masjedizadeh, MR;Bradshaw, JM;Hill, RJ;Tam, D;Bisconte, A;Kim, EO;Francesco, M;Xing, Y;Shu, J;Karr, D;LaStant, J;Finkle, D;Loewenstein, N;Haberstock-Debic, H;Taylor, MJ;Nunn, P;Langrish, CL;Goldstein, DM;Discovery of Reversible Covalent Bruton's Tyrosine Kinase Inhibitors PRN473 and PRN1008 (Rilzabrutinib)Journal of medicinal chemistry35302767The ability of selected compounds to block BCR-driven activation of B cells was assessed by expression of the CD69 surface marker, as previously described. (37) Briefly, HWB (AllCells, Alameda, CA, USA) pretreated for 1 h with serial dilutions of tesFull Paper
2022Zheng, L;Wang, H;Zuo, P;Liu, Y;Xu, H;Ye, BC;Rapid On-Chip Isolation of Cancer-Associated Exosomes and Combined Analysis of Exosomes and Exosomal ProteinsAnalytical chemistry35575685The biotin-labeled rabbit antihuman CD63 antibody (anti-CD63) was purchased from AllCells Biotech Co., Ltd. (Shanghai, China). The horseradish peroxidase (HRP)-conjugated secondary antibody, rabbit anti-human CD9 polyclonal antibody, and bicinchoniniFull Paper
2022Avanzino, BC;Prabhakar, K;Dalvi, P;Hartstein, S;Kehm, H;Balasubramani, A;Boudreau, AA;Buelow, B;Chang, K;Davison, LM;Iyer, S;Kalwit, V;Lewis Wilson, K;Malik-Chaudhry, HK;Pierson, W;Pineda, G;Rangaswamy, US;Saiganesh, S;Schellenberger, U;Ugamraj, HS;Yabut, RD;Buelow, R;Chapman, J;Trinklein, ND;Harris, KE;A T-cell engaging bispecific antibody with a tumor-selective bivalent folate receptor alpha binding arm for the treatment of ovarian cancerOncoimmunology36016696In a similar follow-on study, NCG mice were injected with activated human PBMCs (AllCells). Prior to injection, PBMCs were activated for 3 d with 1 µg/mL plate-coated anti-CD3 ( SaveFull Paper
2022Xue, W;Zhang, Q;Chen, Y;Zhu, Y;Hydrogen Sulfide Improves Angiogenesis by Regulating the Transcription of pri-miR-126 in Diabetic Endothelial CellsCells36078059The HUVECs from passages 4 to 7 were used in the experiment and were cultured in endothelial completed medium (ALLCELLS, Shanghai, China) at 37 C in 5% CO 2 . The HUVECs Save CachedFull Paper
2022Sharma, M;Bellio, MA;Benny, M;Kulandavelu, S;Chen, P;Janjindamai, C;Han, C;Chang, L;Sterling, S;Williams, K;Damianos, A;Batlahally, S;Kelly, K;Aguilar-Caballero, D;Zambrano, R;Chen, S;Huang, J;Wu, S;Hare, JM;Schmidt, A;Khan, A;Young, K;Mesenchymal Stem Cell-derived Extracellular Vesicles Prevent Experimental Bronchopulmonary Dysplasia Complicated By Pulmonary HypertensionStem cells translational medicine35758326es, and isotype control was performed on P2 WJ-MSC to confirm the MSC identity. BM-MSC Production BM-MSCs were isolated from fresh adult bone marrow aspirates, and obtained from a commercial source (AllCells, Alameda, CA). Briefly, bone marrow was pFull Paper
2022Ohori-Morita, Y;Niibe, K;Limraksasin, P;Nattasit, P;Miao, X;Yamada, M;Mabuchi, Y;Matsuzaki, Y;Egusa, H;Novel Mesenchymal Stem Cell Spheroids with Enhanced Stem Cell Characteristics and Bone Regeneration AbilityStem cells translational medicine35267026heir therapeutic potential in bone regeneration. Materials and Methods BM-MSC Adherent Culture Human BM-MSCs of an 18-year-old male (donor 1; Lonza, Basel, Switzerland), a 22-year-old male (donor 2; AllCells, Alameda, CA, USA), and a 24-year-old malFull Paper
2022Stratmann, S;Yones, SA;Garbulowski, M;Sun, J;Skaftason, A;Mayrhofer, M;Norgren, N;Herlin, MK;Sundström, C;Eriksson, A;Höglund, M;Palle, J;Abrahamsson, J;Jahnukainen, K;Munthe-Kaas, MC;Zeller, B;Tamm, KP;Cavelier, L;Komorowski, J;Holmfeldt, L;Transcriptomic analysis reveals proinflammatory signatures associated with acute myeloid leukemia progressionBlood advances34619772children (Table 1). Detailed biological characteristics and supporting clinical information are reported in supplemental Tables 2 and 3. CD34-expressing bone marrow (BM) cells from 5 healthy donors (AllCells Inc., Alameda, CA) were used as normal conFull Paper
2022Jeon, E;Choi, D;Choi, S;Won, J;Jo, Y;Kim, H;Jung, Y;Shin, S;Min, H;Choi, H;Lee, M;Park, Y;Chung, J;Jin, H;Enhancing adoptive T‐cell therapy with fucoidan‐based IL ‐2 delivery microcapsulesBioengineering & Translational MedicineCryopreserved human peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryvill, CA). Total CD3+T cells were isolated by negative selection with a magnetic isolation kit and CD4+T, CD8+T, and regulatory T (Treg) cells were thenFull Paper
2022Lun, Y;Zhou, Y;Li, Q;Chen, P;Huang, Y;Ye, G;Preparation and characterization of a magnetic microsphere synthesized from sucrose allyl ether for transcatheter arterial embolizationMaterials Today ChemistryHuman umbilical vein endothelial cells (HUVECs, AllCells, US) were subcultured in a low glucose Dulbecco's modified eagle medium containing 10% fetal bovine serum and 1% penicillin/streptomycin (100 U·mL−1) in an atmosphere of 310.15 K and 5% CO2Full Paper
2022Lu, W;Zhou, C;Ma, Y;Li, J;Jiang, J;Chen, Y;Dong, L;He, F;Improved osseointegration of strontium-modified titanium implants by regulating angiogenesis and macrophage polarizationBiomaterials science35384947HUVECs together with complete and basal medium were purchased from Allcells Bio (Shanghai, China). The murine macrophage cell lines (Raw264.7) were obtained from the SaveFull Paper
2022Zhou, Y;Li, XH;Xue, WL;Jin, S;Li, MY;Zhang, CC;Yu, B;Zhu, L;Liang, K;Chen, Y;Tao, BB;Zhu, YZ;Wang, MJ;Zhu, YC;YB-1 Recruits Drosha to Promote Splicing of pri-miR-192 to Mediate the Proangiogenic Effects of H2SAntioxidants & redox signaling35044231HUVECs were purchased from Allcells (Shanghai, China) and cultured in endothelial basal medium (EBM; Allcells, Shanghai, China) supplemented with hydrocortisone, bovine brain SaveFull Paper
2022Li, J;Hong, T;Wei, Y;Guo, L;Lee, M;Yang, H;Class, C;Yang, Y;Wang, X;He, H;Siwko, S;You, MJ;Zhou, Y;Garcia-Manero, G;Huang, Y;Aberrant DNA hydroxymethylation reshapes transcription factor binding in myeloid neoplasmsClinical epigenetics35765052BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). BM mononuclear cells were isolated using a Ficoll-Paque density gradient media (GE, Sweden). Save CachedFull Paper
2022Fang, J;Li, R;Zhang, Y;Oduro, PK;Li, S;Leng, L;Wang, Z;Rao, Y;Niu, L;Wu, HH;Wang, Q;Aristolone in Nardostachys jatamansi DC. induces mesenteric vasodilation and ameliorates hypertension via activation of the KATP channel and PDK1-Akt-eNOS pathwayPhytomedicine : international journal of phytotherapy and phytopharmacology35738117Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells Biotech Co. Ltd. (Shanghai, China) and cultured in Endothelial Cell Medium (ECM; AllCells Biotech Co. Ltd., Shanghai, China) with 10% fetal bovine serum, 1% endothelial growtFull Paper
2022Liang, W;Chen, J;Zheng, H;Lin, A;Li, J;Wu, W;Jie, Q;MiR-199a-5p-containing macrophage-derived extracellular vesicles inhibit SMARCA4 and alleviate atherosclerosis by reducing endothelial cell pyroptosisCell biology and toxicology35930100Human monocyte-derived macrophages (hMDMs, PB-MDM-001F, AllCells) were purchased from Milestone Biotechnologies (Shanghai, China). The hMDMs were cultured at 37 °C in RPMI1640 medium (supplemented with 10% FBS and 1% penicillin/streptomycin) with 5%Full Paper
2022Wang, J;Li, C;He, K;Kuang, Z;Lu, J;Yao, Y;He, F;Li, N;Li, L;Fu, F;Wu, Z;Zhou, S;Kang, D;Qiu, X;Wu, M;Liu, Y;Cao, X;Xu, M;Chen, B;Wu, W;Guo, F;Characterization of anti-CD79b/CD3 bispecific antibody, a potential therapy for B cell malignanciesCancer immunology, immunotherapy : CII35963895Materials and methods Reagents IBI38D9 and human IgG1 were produced by Innovent Biologics Co., Ltd. (Suzhou, China). Human IgG was purchased from Equitech-Bio. Peripheral blood mononuclear cells (PBMCs) were purchased from the AllCellsFull Paper
2022Ni, H;Cao, L;Wu, Z;Wang, L;Zhou, S;Guo, X;Gao, Y;Jing, H;Wu, M;Liu, Y;Ding, J;Zhang, P;Zhou, Y;Chen, B;Xiong, Y;Sun, J;Prinz, B;Baruah, H;Geoghegan, J;Yu, M;Wu, W;Liu, J;Combined strategies for effective cancer immunotherapy with a novel anti-CD47 monoclonal antibodyCancer immunology, immunotherapy : CII34165607CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMCs, Allcells) and then differentiated into macrophages following incubation for 7 days with granulocyte- SaveFull Paper
2022Wu, ZH;Li, N;Gao, ZZ;Chen, G;Nie, L;Zhou, YQ;Jiang, MZ;Chen, Y;Chen, J;Mei, XF;Hu, F;Wang, HB;Development of the Novel Bifunctional Fusion Protein BR102 That Simultaneously Targets PD-L1 and TGF-β for Anticancer ImmunotherapyCancers36230887IgG1 isotype control was expressed by Biointron Biological (Taizhou, China). 2.2. Cell Lines and Cell Culture Dendritic cells (DCs) and peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Shanghai, China). TF-1 cells were from tFull Paper
2022Wang, X;Chen, G;Nie, L;Wu, Z;Wang, X;Pan, C;Chen, X;Zhao, X;Zhu, J;He, Q;Wang, H;IL-2K35C-moFA, a Long-Acting Engineered Cytokine with Decreased Interleukin 2 Receptor α Binding, Improved the Cellular Selectivity Profile and Antitumor Efficacy in a Mouse Tumor ModelCancers36230665Next, 100 μL CellTiter-Glo (G7572, Promega Corporation, Madison, WI, USA) was added; luminescence was recorded suing a Spectramax M5. 2.8. p-STAT5 Assay in Treg and CD8+ T Cell Tregs (PB009-4F-2, Allcells, Shanghai, China) at a density of 1 × 106 cFull Paper
2022Sekhri, P;Ledezma, DK;Shukla, A;Sweeney, EE;Fernandes, R;The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma CellsCancers35326601Healthy donor peripheral blood leukopaks were purchased from AllCells (Alameda, CA, USA) along with the HLA report. Peripheral blood mononuclear cells (PBMCs) were isolated by SaveFull Paper
2022Ning, K;Roy, A;Cheng, F;Xu, P;Kleiboeker, S;Escalante, CR;Wang, J;Qiu, J;High-Throughput Screening Identifies Inhibitors for Parvovirus B19 Infection of Human Erythroid ProgenitorsJournal of virology34669461Primary human CD34+ hematopoietic stem cells (HSCs), which were isolated from the bone marrow of a healthy human donor, were purchased from AllCells LLC (Alameda, CA). B19V-containing plasma samples were obtained from ViraCor Eurofins Laboratories (LFull Paper
2022Liu, Y;Tsang, K;Mays, M;Hansen, G;Chiecko, J;Crames, M;Wei, Y;Zhou, W;Fredrick, C;Hu, J;Liu, D;Gebhard, D;Huang, ZF;Datar, A;Kronkaitis, A;Gueneva-Boucheva, K;Seeliger, D;Han, F;Sen, S;Kasturirangan, S;Scheer, JM;Nixon, AE;Panavas, T;Marlow, MS;Kumar, S;An adapted consensus protein design strategy for identifying globally optimal biotherapeuticsmAbs35613320isolated from human peripheral blood mononuclear cells (PBMCs) using a negative selection kit (EasySep). PBMCs were isolated from Leukopak collected from healthy volunteers, which were obtained from ALLcells. Redirected T-cell cytotoxicity was assayeFull Paper
2022Zhao, J;Jiang, L;Yang, H;Deng, L;Meng, X;Ding, J;Yang, S;Zhao, L;Xu, W;Wang, X;Zhu, Z;Huang, H;A strategy for the efficient construction of anti-PD1-based bispecific antibodies with desired IgG-like propertiesmAbs35239451#F9512) at 4°C for 1 h. Cells were washed three times and then analyzed on the CytoFLEX Cytometer System (Beckman Coulter). Allogeneic mixed lymphocyte reaction Monocytes were enriched from PBMCs (AllCells, Cat# PB002-C-300) by adherence on tissue cFull Paper
2022McGuire, BE;Mela, JE;Thompson, VC;Cucksey, LR;Stevens, CE;McWhinnie, RL;Winkler, DFH;Pelech, S;Nano, FE;Escherichia coli recombinant expression of SARS-CoV-2 protein fragmentsMicrobial cell factories35123472mall number of serum or plasma samples from positive tested donors and all pre-COVID-19 samples were acquired by purchase (Precision for Medicine, Norton, MA, USA; Innovative Research, Novi, MI, USA; AllCells, Alameda, CA, USA) or by donation (CureImFull Paper
2022Li, G;Reid, KM;Spitler, K;Beatty, N;Boucher, J;Davila, ML;CD3 engagement as a new strategy for allogeneic "off-the-shelf" T cell therapyMolecular therapy oncolytics35317526lls was harvested, and 0.45 μm was filtered and cryopreserved for future use. For T cell transduction, healthy donor leukopaks were purchased from Stemcell Technologies (Vancouver, BC, Canada) and ALLCELLS (Alameda, CA). PBMCs were isolated using staFull Paper
2022Biscaia, S;Branquinho, MV;Alvites, RD;Fonseca, R;Sousa, AC;Pedrosa, SS;Caseiro, AR;Guedes, F;Patrício, T;Viana, T;Mateus, A;Maurício, AC;Alves, N;3D Printed Poly(????-caprolactone)/Hydroxyapatite Scaffolds for Bone Tissue Engineering: A Comparative Study on a Composite Preparation by Melt Blending or Solvent Casting Techniques and the Influence of Bioceramic Content on Scaffold PropertiesInternational journal of molecular sciences35216432ON 4505 equipment in a dry state at a rate of 1 mm/min. Five specimens were tested for each composition. 4.3. Cytocompatibility Analysis 4.3.1. Cell Culture and Maintenance hDPSCs were acquired from AllCells, LLC (Cat. DP0037F, Lot N° DPSC090411-01)Full Paper
2022Cunningham, AW;Jones, M;Frank, N;Sethi, D;Miller, MM;Stem-like memory T cells are generated during hollow fiber perfusion-based expansion and enriched after cryopreservation in an automated modular cell therapy manufacturing processCytotherapy36031522Leukopaks from three healthy donors were acquired by processing whole blood on the Spectra Optia Apheresis System and obtained through AllCells (Alameda, CA, USA) (Fig. 1A). The apheresis product was shipped to the lab overnight, and CD3+ T cells werFull Paper
2022Jimenez, AC;Heist, CA;Navaei, M;Yeago, C;Roy, K;Longitudinal two-dimensional gas chromatography mass spectrometry as a non-destructive at-line monitoring tool during cell manufacturing identifies volatile features correlative to cell product qualityCytotherapy35882596CellBIND 96-well plate (Corning) for 48-h before co-culture at 50 000 cells per well. PBMCs (AllCells, Alameda, CA, USA) were thawed with a CTL anti-aggregate solution (Cellular Technology Ltd, Shaker Heights, OH, USA) and plated in a six-well plateFull Paper
2022Maughon, TS;Shen, X;Huang, D;Michael, AOA;Shockey, WA;Andrews, SH;McRae, JM;Platt, MO;Fernández, FM;Edison, AS;Stice, SL;Marklein, RA;Metabolomics and cytokine profiling of mesenchymal stromal cells identify markers predictive of T-cell suppressionCytotherapy34696960ethylenediaminetetraacetic acid; Gibco) and then seeded at three densities (10 000, 5000 and 2000 cells/well) in a 96-well plate and cultured for 24 h. PBMCs (AllCells, Alameda, CA, USA) were thawed in Roswell Park Memorial Institute medium, 20% fetaFull Paper
2022Xu, C;Yang, J;Kosters, A;Babcock, BR;Qiu, P;Ghosn, EEB;Comprehensive multi-omics single-cell data integration reveals greater heterogeneity in the human immune systemiScience36185375ical samples ------------------------- Human Adult Peripheral Blood Emory University’s Children’s Clinical and Translational Discovery Core N/A Human Adult Bone Marrow Emory University; AllCells N/A ------------------------- Critical coFull Paper
2022Magis, W;DeWitt, MA;Wyman, SK;Vu, JT;Heo, SJ;Shao, SJ;Hennig, F;Romero, ZG;Campo-Fernandez, B;Said, S;McNeill, MS;Rettig, GR;Sun, Y;Wang, Y;Behlke, MA;Kohn, DB;Boffelli, D;Walters, MC;Corn, JE;Martin, DIK;High-level correction of the sickle mutation is amplified in vivo during erythroid differentiationiScience35633935Cryopreserved CD34+ cells were thawed according to AllCells instructions and cultured for 2 days in StemSpan SFEM with CC110 supplement (StemCell Technologies). For each Save;. Methods CD34+cells CD34+ HSPCs homozygous for the sickle mutation wereFull Paper
2022Liu, P;Qin, L;Liu, C;Mi, J;Zhang, Q;Wang, S;Zhuang, D;Xu, Q;Chen, W;Guo, J;Wu, X;Exosomes Derived From Hypoxia-Conditioned Stem Cells of Human Deciduous Exfoliated Teeth Enhance Angiogenesis via the Transfer of let-7f-5p and miR-210-3pFrontiers in cell and developmental biology35557954Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Cat. H-001F, AllCells) and were cultured in DMEM (Cat. SH30021.01, Hyclone) supplemented with 10 SaveFull Paper
2022Li, L;Zhou, B;Xu, H;Shi, H;Gao, L;Ge, B;Zinc-Loaded Black Phosphorus Multifunctional Nanodelivery System Combined With Photothermal Therapy Have the Potential to Treat Prostate Cancer Patients Infected With COVID-19Frontiers in endocrinology35464050n, USA). Human HMGB1 ELISA Kit was obtained from Arigo Biolaboratories (Taiwan, China). ATP content detection kit was obtained from Solarbio (Beijing, China). Dendritic cells (DCs) were obtained from AllCells (San Francisco, USA). FITC anti-human CD1Full Paper
2022McGovern, K;Castro, AC;Cavanaugh, J;Coma, S;Walsh, M;Tchaicha, J;Syed, S;Natarajan, P;Manfredi, M;Zhang, XM;Ecsedy, J;Discovery and Characterization of a Novel Aryl Hydrocarbon Receptor Inhibitor, IK-175, and Its Inhibitory Activity on Tumor Immune SuppressionMolecular cancer therapeutics35666806Naive human T cells (CD4+/CD62L+) from two independent donors were isolated from cryopreserved CD4+ T cells (AllCells) by magnetic selection with human CD62 L microbeads (Miltenyi). On Day 1, naive CD62L+ human T cells were activated with human CD3/CFull Paper
2022Lin, W;Qian, J;Wang, H;Ren, L;Yang, Y;Chen, C;Chen, X;Huang, Y;Liu, J;Xu, N;Teng, L;Cisplatin plus anti-PD-1 antibody enhanced treatment efficacy in advanced esophageal squamous cell carcinomaAmerican journal of cancer research35261780Therapies for patients with advanced esophageal squamous cell carcinoma (ESCC) are limited and accompanied by dismal prognosis. Here we use ESCC cell line K30 and TE-1 to SaveFull Paper
2022Duan, J;Lei, D;Ling, C;Wang, Y;Cao, Z;Zhang, M;Zhang, H;You, Z;Yao, Q;Three-dimensional-printed polycaprolactone scaffolds with interconnected hollow-pipe structures for enhanced bone regenerationRegenerative biomaterials35719204CR MasterMix (ABM, CA) on a Light Cycler 480 II (Roche, CHE). To evaluate the expression of angiogenesis genes (HIF1-α and VEGF), human umbilical vein endothelial cells (HUVECs) were purchased from AllCells. First, 6 × 105 HUVECs were seeded verticaFull Paper
2022Li, Z;Kou, L;Fu, X;Xie, Z;Xu, M;Guo, L;Lin, T;Gong, S;Zhang, S;Liu, M;Design, synthesis, and biological evaluation of triazole-pyrimidine-methylbenzonitrile derivatives as dual A2A/A2B adenosine receptor antagonistsJournal of enzyme inhibition and medicinal chemistry35616298Cryopreserved PBMCs (Allcells) were thawed and recovered in RPMI1640 medium overnight before being used. And compounds to be tested were diluted with DMSO to 100 folds of SaveFull Paper
2022Hu, S;Muniraj, G;Mishra, A;Hong, K;Lum, JL;Hong, CHL;Rosa, V;Sriram, G;Characterization of silver diamine fluoride cytotoxicity using microfluidic tooth-on-a-chip and gingival equivalentsDental materials : official publication of the Academy of Dental Materials35778310Objective This study aims to characterize the cytotoxicity potential of silver diamine fluoride (SDF) on dental pulp stem cells (DPSC) and gingival equivalents. Methods DPSC cultured on SaveFull Paper
2022Dogan, M;Karhan, E;Kozhaya, L;Placek, L;Chen, X;Yigit, M;Unutmaz, D;Engineering Human MAIT Cells with Chimeric Antigen Receptors for Cancer ImmunotherapyJournal of immunology (Baltimore, Md. : 1950)36165183Healthy adult blood was obtained from AllCells (Quincy, MA). PBMCs were isolated using Ficoll-Paque plus (GE Healthcare). CD4 + T, CD8 + T, and CD19 + B cells and CD14 + SaveFull Paper
2022Ben Nasr, M;Robbins, D;Parone, P;Usuelli, V;Tacke, R;Seelam, AJ;Driver, E;Le, T;Sabouri-Ghomi, M;Guerrettaz, L;Shoemaker, D;Fiorina, P;Pharmacologically Enhanced Regulatory Hematopoietic Stem Cells Revert Experimental Autoimmune Diabetes and Mitigate Other Autoimmune DisordersJournal of immunology (Baltimore, Md. : 1950)35321879A total of 5000 cryopreserved and preisolated human mobilized peripheral blood HSCs (CD34 + cells) (AllCells) were plated into each well of a 384-well plate (Corning) in StemSpan- SaveFull Paper
2022Melani, RD;Des Soye, BJ;Kafader, JO;Forte, E;Hollas, M;Blagojevic, V;Negrão, F;McGee, JP;Drown, B;Lloyd-Jones, C;Seckler, HS;Camarillo, JM;Compton, PD;LeDuc, RD;Early, B;Fellers, RT;Cho, BK;Mattamana, BB;Goo, YA;Thomas, PM;Ash, MK;Bhimalli, PP;Al-Harthi, L;Sha, BE;Schneider, JR;Kelleher, NL;Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody RepertoireJournal of proteome research34878788odium heparin (BD 367874, Fisher Scientific) by centrifugation at 1,500 × g for 10 min. Plasma from a convalescent patient featuring a high titer of anti-SARS-CoV-2-RBD antibodies was purchased from AllCells (commercial sample 1 - CS1) and used as aFull Paper
2022Kawato, Y;Fukahori, H;Nakamura, K;Kanno, A;Kubo, K;Hiramitsu, M;Matsuda, T;Hanada, Y;Furukawa, T;Nakajima, Y;Kinugasa, F;Morokata, T;Potential benefit of the cathepsin S inhibitor, ASP1617, as a treatment for systemic lupus erythematosusEuropean journal of pharmacology35157914Five different lots of peripheral blood-derived frozen B cell were purchased from AllCells (Alameda, CA, USA). Cell suspensions (final, 2 × 10 6 SaveFull Paper
2022Pieles, O;Höring, M;Adel, S;Reichert, TE;Liebisch, G;Morsczeck, C;Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle CellsStem cells international35903407Human dental follicle cells were purchased from AllCells (Alameda, CA, USA) and cultured in Dulbecco's Modified Eagle's Medium (DMEM) containing 10% fetal bovine serum (FBS) SaveFull Paper
2022Schlichtner, S;Yasinska, IM;Ruggiero, S;Berger, SM;Aliu, N;Prunk, M;Kos, J;Meyer, NH;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV;Expression of the Immune Checkpoint Protein VISTA Is Differentially Regulated by the TGF-β1 - Smad3 Signaling Pathway in Rapidly Proliferating Human Cells and T LymphocytesFrontiers in medicine35223897Medium was also supplemented with penicillin (50 IU/ml), and streptomycin sulfate (50 μg/ml). Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were also purchased from AllCells (Alameda, CA, USA) and handled according toFull Paper
2022Yoshimaru, K;Yamaza, T;Kajioka, S;Sonoda, S;Yanagi, Y;Matsuura, T;Yoshizumi, J;Oda, Y;Iwata, N;Takai, C;Nakayama, S;Taguchi, T;Dental pulp stem cells as a therapy for congenital entero-neuropathyScientific reports35484137ies were passaged to expand the number of dDPSCs. Cells at P3 were used for the experiments. BMMSCs isolated from human whole bone marrow aspirates obtained from healthy adult volunteers (n = 3; AllCells, Alameda, CA, USA) and human skin fibroblastsFull Paper
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2022Kim, DE;Shin, YH;Cho, JE;Myung, S;Kim, HG;Kim, KC;Park, CM;Yoon, CH;Identification of 3-Oxindole Derivatives as Small Molecule HIV-1 Inhibitors Targeting Tat-Mediated Viral TranscriptionMolecules (Basel, Switzerland)35956872d fetal bovine serum (all obtained from Gibco-BRL, Gaithersburg, MD, USA). The bl-DTR cells were additionally supplemented with 1 μg/mL puromycin and 200 μg/mL zeocin. The PBMCs were purchased from AllCells (Alameda, CA, USA) and cultured, as describFull Paper
2022Saaid, A;Ensanya, A;Suzina, S;Nurul, A;Samsudin, A;Azlina, A;Odontogenic induction of human amniotic membrane scaffold for dental pulp regenerationMaterials Chemistry and PhysicsFor this study, deciduous teeth stem cells (DTSCs), obtained from ALLCELLS, USA, were used at passage 4-8. The cells were allowed to grow in a-modified Eagle's medium (α-MEM) supplemented with 20% fetal bovine serum (FBS) and 1% penicillin/streptomycFull Paper
2022Jaimes, MC;Leipold, M;Kraker, G;Amir, EA;Maecker, H;Lannigan, J;Full spectrum flow cytometry and mass cytometry: A 32-marker panel comparisonCytometry. Part A : the journal of the International Society for Analytical Cytology35593221Frozen PBMCs from 5 donors were obtained from AllCells Alameda (Alameda, CA). Cells were provided aliquoted in 25 million cells per vial in freezing media and cryopreserved in liquid nitrogen until ready to use. Ethical review and regulatory complianFull Paper
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2022Zhang, H;Lv, X;Kong, Q;Tan, Y;IL-6/IFN-γ double knockdown CAR-T cells reduce the release of multiple cytokines from PBMCs in vitroHuman vaccines & immunotherapeutics35049413rward TGGGAGCATGTGAATGCCATCC Reverse GTCGGCTCCTGGAGGTCAAACA β-actin Forward CGCCCCAGGCACCAGGGC Reverse GCTGGGGTGTTGAAGGT 2.3. IL-6/IFN-γ double KD CAR-T cell culture The PBMCs (Allcells, Cat: PB005F) were resuspended in CTSTM AIMFull Paper
2022Zhou, Z;Qin, M;Khodahemmati, S;Li, W;Niu, B;Li, J;Liu, Y;Gao, J;Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysisInternational journal of environmental health research34102927We maintained HUVECs (Allcells, China) routinely in HUVEC complete culture medium (Allcells, China), supplemented with 10% fetal bovine serum (FBS), 2% growth factors, 100 U/mL SaveFull Paper
2022Wang, X;Wong, LM;McElvain, ME;Martire, S;Lee, WH;Li, CZ;Fisher, FA;Maheshwari, RL;Wu, ML;Imun, MC;Murad, R;Toledo Warshaviak, D;Yin, J;Kamb, A;Xu, H;A rational approach to assess off-target reactivity of a dual-signal integrator for T cell therapyToxicology and applied pharmacology35085592Informed-consent for use of primary T cells and donor collection protocols was approved by an Institutional Review Board (IRB) at Allcells or HemaCare. Allcells and HemaCare SaveFull Paper
2022Andrews, SH;Klinker, MW;Bauer, SR;Marklein, RA;Morphological landscapes from high content imaging reveal cytokine priming strategies that enhance mesenchymal stromal cell immunosuppressionBiotechnology and bioengineering34716713Human bone marrow-derived MSCs were obtained from four different donors purchased from Lonza, AllCells, and RoosterBio (see Table S1 for donor information). MSC culture conditions for the Lonza and AllCells (8F3560, 110877, PCBM1662) were chosen baseFull Paper
2022Mandal, S;Sunagawa, SW;Prathipati, PK;Belshan, M;Shibata, A;Destache, CJ;Targeted Immuno-Antiretroviral to Promote Dual Protection against HIV: A Proof-of-Concept StudyNanomaterials (Basel, Switzerland)35683795were maintained in a complete DMEM medium (HiDMEM medium supplemented with 10% FBS and 1× AA) as standard protocol [14,15]. The human peripheral blood mononuclear cells (hPBMCs) were purchased from AllCells (Alameda, CA, USA) and maintained in compFull Paper
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2022Harper, T;Sharma, A;Kaliyaperumal, S;Fajardo, F;Hsu, K;Liu, L;Davies, R;Wei, YL;Zhan, J;Estrada, J;Kvesic, M;Nahrwold, L;Deisting, W;Panzer, M;Cooke, K;Lebrec, H;Nolan-Stevaux, O;Characterization of an Anti-CD70 Half-Life Extended Bispecific T-Cell Engager (HLE-BiTE) and Associated On-Target Toxicity in Cynomolgus MonkeysToxicological sciences : an official journal of the Society of Toxicology35583313Human T cells (AllCells) or cynomolgus monkey or human peripheral blood mononuclear cells (PBMCs) (SNBL) were cocultured with target cells at an effector cell (E) to target cell (T) SaveFull Paper
2022Wang, P;Zhang, J;Zhang, H;Zhang, F;The role of MACF1 on acute myeloid leukemia cell proliferation is involved in Runx2-targeted PI3K/Akt signalingMolecular and cellular biochemistry35857251Bone marrow CD34 + hematopoietic stem and progenitor cells (HSPCs) were purchased from AllCells (Alameda, CA, USA) and cultured in X-Vivo 15 media (Lonza, Basel, Switzerland) SaveFull Paper
2022Shams, F;Bayat, H;Mohammadian, O;Mahboudi, S;Vahidnezhad, H;Soosanabadi, M;Rahimpour, A;Advance trends in targeting homology-directed repair for accurate gene editing: An inclusive review of small molecules and modified CRISPR-Cas9 systemsBioImpacts : BI35975201A) Treatment group 1A (unedited HSCs from AllCells). The first mouse was found dead in Treatment group 2B, which contained edited bought HSCs (AllCells), had the highest bone SaveFull Paper
2022Liu, Y;Ma, X;Yang, H;Li, X;Ma, Y;Ason, B;Liu, S;Hu, LA;APLNR Regulates IFN-γ signaling via β-arrestin 1 mediated JAK-STAT1 pathway in melanoma cellsThe Biochemical journal35084016Naive CD8+ T cells used for co-culture assay were obtained from Allcells and cultured in RPMI 1640 medium supplemented with 10% FBS, 1× HEPES, 1× PS, 2 mM Glutamine, 1 mM SaveFull Paper
2022Venn-Watson, SK;Butterworth, CN;Broader and safer clinically-relevant activities of pentadecanoic acid compared to omega-3: Evaluation of an emerging essential fatty acid across twelve primary human cell-based disease systemsPloS one35617322lowed ethical guidelines and documented informed consent: Cell Applications, Inc., CellzDirect, Celsis-IVT, Leukolab, Life Technologies, Lonza, ScienCell, Hemacare Corporation, Stemcell Technologies, AllCells, Physician’s Plasma Alliance, Lifeline CeFull Paper
2022Okada, Y;Kawashima, N;Noda, S;Murano, H;Han, P;Hashimoto, K;Kaneko, T;Okiji, T;VEGFA promotes odonto/osteoblastic differentiation in dental pulp stem cells via ERK/p38 signalingJournal of Dental SciencesSHEDs (P5, DP001F; AllCells, Alameda, CA, USA) were cultured in alpha-modified Eagle's minimum essential medium (α-MEM) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA) and an antibiotic and antifungal solutiFull Paper
2022Chen, W;Qie, C;Hu, X;Wang, L;Jiang, J;Liu, W;Liu, J;A small molecule inhibitor of VSIG-8 prevents its binding to VISTAInvestigational new drugs35404016PBMCs were purchased from Allcells Biotechnology (Shanghai) Co., LTD, and we got a statement confirming that informed consent was obtained from all subjects, the attachment is on response. Jurkat cells were cultured in RPMI-1640 medium (Biological InFull Paper
2022Han, Y;Bai, X;Wang, X;Exosomal myeloperoxidase as a biomarker of deep venous thrombosisAnnals of translational medicine35242854al microscope (Olympus). HUVECs culture We purchased HUVECs from American Type Culture Collection and cultured them in endothelial cell complete medium containing endothelial cell growth supplement (AllCells, Alameda, CA, USA) at 37 °C in a humidifiFull Paper
2022Sato, M;Fujii, K;Takagi, H;Shibuya, I;Oka, D;Yamaya, N;Hagita, H;Matsumoto, M;Inagaki, K;Antibacterial and Immunosuppressive Effects of OPS-2071, a Candidate Therapy for Inflammatory Bowel DiseaseDigestive diseases and sciences34463880Mouse splenocytes were prepared from eight-week-old female BALB/c mice, and human peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Alameda, CA). To SaveFull Paper
2022Skaddan, MB;Wooten, DW;Wilcox, KC;Voorbach, MJ;Reuter, DR;Jia, ZJ;Foster-Duke, KD;Hickson, JA;Vaidyanathan, S;Reed, AD;Tovcimak, AE;Guo, Q;Comley, RA;Lee, L;Finnema, SJ;Mudd, SR;[18F]BTK-1: A Novel Positron Emission Tomography Tracer for Imaging Bruton's Tyrosine KinaseMolecular imaging and biology35482146Purpose Bruton's tyrosine kinase (BTK) is a key component of B cell receptor (BCR) signaling, and as such a critical regulator of cell proliferation and survival. Aberrant BCR signaling is SaveFull Paper
2022Wu, C;Wang, X;Shang, H;Wei, H;Construction of a Humanized PBMC-PDX Model to Study the Efficacy of a Bacterial Marker in Lung Cancer ImmunotherapyDisease markers36072895, Ltd., and the RPMI-1640 Complete Medium (10%FBS; Gibco) was used for culture. When the cells reached 80% confluence, they were passed on. 2.3. PBMCs Immune Reconstitution PBMCs were purchased from AllCells and processed according to the company'sFull Paper
2022Ryan, DE;Diamant-Levi, T;Steinfeld, I;Taussig, D;Visal-Shah, S;Thakker, S;Lunstad, BD;Kaiser, RJ;McCaffrey, R;Ortiz, M;Townsend, J;Welch, WRW;Singh, M;Curry, B;Dellinger, DJ;Bruhn, L;Phosphonoacetate Modifications Enhance the Stability and Editing Yields of Guide RNAs for Cas9 EditorsBiochemistry35436085Human primary T cells (LP, CR, CD3+, NS) were obtained from AllCells (Alameda, CA) and were cultured in RPMI 1640 plus GlutaMax media supplemented with 10% fetal bovine serum, 5 ng/mL human IL-7, and 5 ng/mL human IL-15 (Gibco). Primary T cells wereFull Paper
2022Darbaniyan, F;Zheng, H;Kanagal-Shamanna, R;Lockyer, P;Montalban-Bravo, G;Estecio, M;Lu, Y;Soltysiak, KA;Chien, KS;Yang, H;Sasaki, K;Class, C;Ganan-Gomez, I;Do, KA;Garcia-Manero, G;Wei, Y;Transcriptomic Signatures of Hypomethylating Agent Failure in Myelodysplastic Syndromes and Chronic Myelomonocytic LeukemiaExperimental hematology36150563CD34 + cells isolated from healthy individuals (AllCells, Alameda CA) and from patients with MDS or CMML were plated into human methylcellulose colony formation medium Methocult- SaveFull Paper
2022Valdez, BC;Yuan, B;Murray, D;Nieto, Y;Popat, U;Andersson, BS;Enhanced cytotoxicity of bisantrene when combined with venetoclax, panobinostat, decitabine and olaparib in acute myeloid leukemia cellsLeukemia & lymphoma35188042Bisantrene (Bis), a topoisomerase-II inhibitor, is less cardiotoxic than the current anthracyclines. Its synergistic cytotoxicity with newly developed antineoplastic drugs has not been SaveFull Paper
2022Fan, F;Liu, F;Shen, P;Tao, L;Zhang, H;Wu, H;Salvianolic acid B, a new type I IRE1 kinase inhibitor, abrogates AngII-induced angiogenesis by interacting with IRE1 in its active conformationClinical and experimental pharmacology & physiology36153795Primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China) and cultured in EBM media (AllCells ) according to the manufacturer's SaveFull Paper
2022Tong, Q;Liu, H;Qi, Q;Dai, C;Yang, T;Qian, F;Development of a fully human anti-GITR antibody with potent antitumor activity using H2L2 miceFEBS open bio35674216h well, and luminescence values were measured using a Varioskan LUX (Thermo, Carlsbad, CA, USA). T‐cell activation assays Total T cells from frozen human peripheral blood mononuclear cells (PBMCs, AllCells, Alameda, CA, USA) were first isolated usinFull Paper
2022Yang, M;Tkach, D;Boyne, A;Kazancioglu, S;Duclert, A;Poirot, L;Duchateau, P;Juillerat, A;Optimized two-step electroporation process to achieve efficient nonviral-mediated gene insertion into primary T cellsFEBS open bio34510816ineering in human primary cells and provide a foundation for further advances using nonviral gene editing methods. Materials and methods T‐cell culture Cryopreserved human PBMCs were acquired from ALLCELLS. PBMCs were cultured in X‐vivo‐15 media (LoFull Paper
2022Pieles, O;Reichert, TE;Morsczeck, C;Protein kinase A is activated during bone morphogenetic protein 2-induced osteogenic differentiation of dental follicle stem cells via endogenous parathyroid hormone-related proteinArchives of oral biology35338829Human dental follicle stem cells (Catalog number FT002F, Lot number ACF001) were commercially obtained from AllCells (Emeryville, USA) and cultured in Dulbecco's Modified Eagle's Medium (DMEM) high glucose (Sigma Aldrich, St. Louis, USA) with 10% fetFull Paper
2022Qian, J;Yu, J;Zhu, X;Liang, S;MiR-335 promotes corneal neovascularization by Targeting EGFRBMC ophthalmology35701740surgery and on day 3, 7, 11 and 14. The Animal Research Committee of the Affiliated Hospital of Nantong University approved all animal studies. Cell culture and different treatments HUVECs were from AllCells (Shanghai, China). They were put in an RPFull Paper
2022Wang, W;Tang, W;Shan, E;Zhang, L;Chen, S;Yu, C;Gao, Y;MiR-130a-5p contributed to the progression of endothelial cell injury by regulating FASEuropean journal of histochemistry : EJH35638591cells (HUVEC) in vitro. Materials and Methods Cell culture HUVEC cells have played a major role as a model system for the study of the regulation of endothelial cell function. HUVEC cells (Shanghai AllCells Biotech Co., Ltd. Shanghai, China) were cuFull Paper
2022Koh, H;Zhen, X;Kim, J;Ha, HY;Lee, JH;Generation and characterization of human umbilical cord blood-derived induced pluripotent stem cells (KRIBBi005-A)Stem cell research35085946Ethical approval Approval obtained by Public Institutional Bioethics Committee designated by the MOHW (P01-201909-31-002). Cord blood mononuclear cells were purchased (AllCells LLC)Full Paper
2022Hashim, SNM;Azlina, A;Ultrastructural morphological changes of VEGF treated-stem cells from human exfoliated deciduous teeth cultured on human amniotic membraneActa MicroscopicaSHED (AllCells, USA) were cultured in a complete medium made of the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, USA), supplemented with 15% of fetal bovine serum (FBS) (Gibco, USA) and 50 U/ml of penicillin-stFull Paper
2022Bai, Y;Zhang, X;Zheng, J;Liu, Z;Yang, Z;Zhang, X;Overcoming high level adenosine-mediated immunosuppression by DZD2269, a potent and selective A2aR antagonistJournal of experimental & clinical cancer research : CR36229853bR, CHO-A1R and CHO-A3R) were purchased from GenScript. Cell lines RM-1, B16F10 and Pan02 were obtained from the American Type Culture Collection (ATCC). Cryopreserved human PBMCs were purchased from AllCells. All CHO cells were cultured in Ham’s F12Full Paper
2022Kartha, VK;Duarte, FM;Hu, Y;Ma, S;Chew, JG;Lareau, CA;Earl, A;Burkett, ZD;Kohlway, AS;Lebofsky, R;Buenrostro, JD;Functional inference of gene regulation using single-cell multi-omicsCell genomics36204155AILS Human peripheral blood mononuclear cells Cryopreserved human peripheral blood mononuclear cells (PBMCs) and isolated peripheral blood CD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from AllCells (see Table S1 for catalog numbers and donFull Paper
2022Riaz, S;Azlina, A;Mahmood, Z;Htun, AT;Long-term treatment of dentine with triple antibiotic paste promotes stem cell viability and attachmentJournal of Taibah University Medical Sciences35983454, the dentine chips were removed from the well plates and air-dried on a filter paper. The samples were then stored in a sterile container at 4 °C until further use. Cell culture Commercial DPSCs (AllCells, USA) were used from passage 7 to 9 at 70%-Full Paper
2022Salam, N;Gibson, IR;Lithium ion doped carbonated hydroxyapatite compositions: Synthesis, physicochemical characterisation and effect on osteogenic response in vitroBiomaterials advances35939955the American Type Culture Collection (via LGC standards, UK), PromoCell (Germany), and AllCells (USA), respectively. Cells were maintained in routine culture conditions using: high glucose DMEM with 10 % FBS and 4 mM l-glutamine for C2C12 cells, GibcFull Paper
2022Nakauchi, Y;Azizi, A;Thomas, D;Corces, MR;Reinisch, A;Sharma, R;Cruz Hernandez, D;Köhnke, T;Karigane, D;Fan, A;Martinez-Krams, D;Stafford, M;Kaur, S;Dutta, R;Phan, P;Ediriwickrema, A;McCarthy, E;Ning, Y;Phillips, T;Ellison, CK;Guler, GD;Bergamaschi, A;Ku, CJ;Levy, S;Majeti, R;The Cell Type-Specific 5hmC Landscape and Dynamics of Healthy Human Hematopoiesis and TET2-Mutant PreleukemiaBlood cancer discovery35532363Normal donor human BM cells were obtained fresh from AllCells, and peripheral blood cells Human BM samples (CD34+ cell enriched) were purchased from AllCells. Mononuclear SaveFull Paper
2022Baskar, R;Chen, AF;Favaro, P;Reynolds, W;Mueller, F;Borges, L;Jiang, S;Park, HS;Kool, ET;Greenleaf, WJ;Bendall, SC;Integrating transcription-factor abundance with chromatin accessibility in human erythroid lineage commitmentCell reports methods35463156# ab181544 Cleaved caspase 3-PE BD Biosciences Cat# 550821, RRID:AB_393906 ------------------------- Biological samples ------------------------- Adult bone marrow All Cells Inc https://allcells.com/research-grade-tissue-products/bone-mFull Paper
2022Kwak, J;Choi, W;Bae, Y;Kim, M;Choi, S;Oh, W;Jin, H;HLA-A2 Promotes the Therapeutic Effect of Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Hyperoxic Lung InjuryBioengineering (Basel, Switzerland)35447737St. Louis, MO, USA) for 1 h at 37 °C. The UCB-MSCs (1 × 103 cells/well) were seeded and maintained at 37 °C in a humidified incubator for 4 h and then co-cultured with PBMCs (1 × 105 cells/well; Allcells, Boston, MA, USA) from different donors. PhytoFull Paper
2022Vivanco Gonzalez, N;Oliveria, JP;Tebaykin, D;Ivison, GT;Mukai, K;Tsai, MM;Borges, L;Nadeau, KC;Galli, SJ;Tsai, AG;Bendall, SC;An optimized protocol for phenotyping human granulocytes by mass cytometrySTAR protocols35434655y donors (with unknown allergy status) were obtained from the Stanford Blood Center (Palo Alto, CA, USA). Healthy bone marrow and peripheral blood samples paired from the same donor were ordered from AllCells (Alameda, CA, USA). Samples from patientsFull Paper
2022Bell-Glenn, S;Thompson, JA;Salas, LA;Koestler, DC;A Novel Framework for the Identification of Reference DNA Methylation Libraries for Reference-Based Deconvolution of Cellular MixturesFrontiers in bioinformatics35419567Cell Mixture Reconstruction Experiment Purified cells taken from normal human subjects were purchased from AllCells LLC (Emeryville, CA). Namely, granulocytes, monocytes, CD4T, SaveFull Paper
2022Shui, S;Wang, S;Liu, J;Systematic Investigation of the Effects of Multiple SV40 Nuclear Localization Signal Fusion on the Genome Editing Activity of Purified SpCas9Bioengineering (Basel, Switzerland)35200436s medium (DMEM) supplemented with 10% (v/v) FBS, 100 U/mL penicillin and 100 U/mL streptomycin. The medium of EGFP reporter contained an additional 50 U/mL hygromycin. Human dermal fibroblasts (hDF, AllCells) were acquired from Cell Applications (LotFull Paper
2022Li, L;Xu, H;Gao, L;Doxorubicin-Loaded Black Phosphorus Multifunctional Nanodelivery System Combined with Photothermal Therapy Promotes Immunogenic Death of Prostate Cancer PC-3 CellsSSRN Electronic JournalBeijing, China). Dendritic cells (DCs) were 197obtained from AllCells (San Francisco, USA). FITC anti198human CD11c Antibody, Alexa Fluor® 647 anti-human 199CD80 Antibody, PE anti-human CD86 Antibody, and 200PerCP/Cyanine5.5 anti-human HLA-DR AntibodFull Paper
2022Calderon, A;Kim, Y;Favaro, P;Baskar, R;Valainis, J;Reynolds, W;Borges, L;Glass, D;Tsai, A;Bendall, S;Multi-Omic Single Cell Identification of Lin- CD34- Natural Killer Cell Progenitors in Human HematopoiesisSSRN Electronic JournalDeidentified human blood (n=5) and BM (n=7) were obtained from healthy adult donors (Stanford Blood Center, Allcells). All samples were obtained under informed consent and in SaveFull Paper
2022Lee, J;Jonus, H;Sadanand, A;Branella, G;Maximov, V;Suttapitugsakul, S;Schniederjan, M;Shim, J;Parwani, K;Fedanov, A;Pilgrim, A;Silva, J;Doering, C;Wu, R;Spencer, H;Goldsmith, K;Identification and Targeting of Protein Tyrosine Kinase 7 (PTK7) as an Immunotherapy Candidate for Neuroblastoma Cellular TherapySSRN Electronic JournalGD2-targeted immunotherapy improves survival for pediatric patients with neuroblastoma (NB). However, toxicities associated with on-target, off-tumor activity occur. Furthermore, GD2 SaveFull Paper
2022O'Brien, G;Cecotka, A;Manola, K;Pagoni, M;Polańska, J;Badie, C;Epigenetic Signature of Ionising Radiation In Therapy-Related AML PatientsSSRN Electronic JournalBone marrow aspirate samples were also obtained from 5 healthy normal donors (4 males, 1 female, age 21-33) from AllCells® (Alamada, USA). The project was in accordance with the declaration of Helsinki. Informed consent was provided from patients andFull Paper
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2022Li, W;Li, J;Hu, X;Xu, L;Liu, X;Qian, Z;Jin, L;Zhang, Y;Wei, J;Liu, X;Tumor suppression by Chinese cordyceps extract via anti-angiogenesisResearch SquareHuman umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in special medium for HUVEC cells at 37C with 5% CO2. CCE was SaveFull Paper
2022Li, L;Chen, X;Li, Y;Lou, L;Pharmacological characterization of GR1803, a novel BCMA × CD3 bispecific antibody for multiple myeloma treatmentResearch SquareThe MM cell lines NCI-H929, MM.1S, RPMI-8226, and U266 were obtained from the American Type Culture Collection (Manassas, VA, USA). Fresh peripheral blood mononuclear cells (PBMCs) were obtained from AllCells Co. (Shanghai, China). Natural killer (NKFull Paper
2022Yan, F;Luo, B;Chen, C;Tan, B;Cui, D;Wang, M;Qian, J;Inhibiting PHD2 in bone marrow mesenchymal stem cells in an inflammatory microenvironment facilitates periodontal repair in SD ratsResearch SquarePrimary BMMSCs of SD rats were isolated and puri ed by AllCells (Alameda, CA, USA), and their phenotype was identi ed. The cells were recovered and cultured in low glucose SaveFull Paper
2022Guedes, F;Branquinho, M;Biscaia, S;Alvites, R;Sousa, A;Lopes, B;Sousa, P;Rêma, A;Amorim, I;Faria, F;Patrício, T;Bugalho, A;Alves, N;Maurício, A;Gamma Irradiation Processing on 3D PCL Devices - A Preliminary Biocompatibility AssessmentpreprintsHuman Dental Pulp stem/stromal cells (hDPSCs) obtained from AllCells, LLC (Cat. DP0037F, Lot Nº DPSC090411-01) were maintained in MEM α, GlutaMAX™ Supplement, no nucleosides (Gibco, 32561029), supplemented with 10% (v/v) fetal bovine serum (FBS) (GibFull Paper
2022Tokatlian, T;Asuelime, G;Naradikian, M;Mock, J;Daris, M;Martin, A;Toledo Warshaviak, D;Kamb, A;Hamburger, A;Chimeric Antigen Receptors Directed at Mutant KRAS Exhibit an Inverse Relationship Between Functional Potency and Neoantigen SelectivityCancer Research CommunicationsPeripheral blood mononuclear cells (PBMC) from healthy donors were obtained from AllCells. PBMC collection and donor written informed consent were approved by an Institutional SaveFull Paper
2022Boccacci, Y;Dumont, N;Doyon, Y;Laganière, J;Accessory-cell-free differentiation of hematopoietic stem and progenitor cells into mature red blood cellsbioRxivThe culture and ex vivo engineering of red blood cells (RBCs) can help characterize genetic variants, model diseases, and may eventually spur the development of applications in SaveFull Paper
2022Checkley, M;Luttge, B;Dobrowolski, C;Wald, D;McMahon, D;Haidar, G;Sobolewski, M;Enick, P;Cyktor, J;Mellors, J;Karn, J;Reduction of the HIV-1 reservoir in T cells from persons with HIV-1 on suppressive antiretroviral therapy using expanded natural killer cells ex vivobioRxivFor this study leukapheresis packs from 5 HIV-1-negative donors were purchased from AllCells. 437 For HIV+ participants, PBMCs from well-suppressed ART-treated patients from the SaveFull Paper
2022Shabaneh, T;Moffett, H;Stull, S;Derezes, T;Tait, L;Park, S;Riddell, S;Lajoie, M;Safety switch optimization enhances antibody-mediated elimination of CAR T cellsbioRxivNatural killer cells were isolated from cryopreserved, T cell depleted (CD4-/CD8-) PBMC (AllCells) by negative selection using the EasySep Human NK Cell Kit (Stemcell) according to the manufacturer’s protocol. To activate their cytolytic function, isFull Paper
2022Wisdom, K;Suijker, J;van den Broek, L;Sridharan, B;Grandhi, T;Cheng, A;Lamb, M;Titus, S;Gehman, A;Poore, D;Shah, N;Cheng, S;Kim, E;Griffin, S;Ekert, J;Lung Tumor Microphysiological System with 3D Endothelium to Evaluate Modulators of T-Cell InfiltrationbioRxivAllCells and shipped to the MIMETAS research facility, or they 653 were isolated from AllCells For this, T-cells were isolated from full 654 fresh leukopaks (AllCells). Leukopaks were SaveFull Paper
2022Dogan, M;Karhan, E;Kozhaya, L;Placek, L;Chen, X;Yigit, M;Unutmaz, D;Engineering human Mucosal Associated Invariant T (MAIT) cells with chimeric antigen receptors for cancer immunotherapy1bioRxivHealthy adult blood was obtained from AllCells Quincy, MA. PBMCs were isolated using Ficoll- 100 paque plus (GE Health care). CD4+ T, CD8+ T, CD19+ B cells, CD14+ monocytes SaveFull Paper
2022Swift, M;Horns, F;Quake, S;Fate Bias and Transcriptional Memory of human B cellsbioRxivfrom a healthy male aged 50-55 were obtained from AllCells. Mononuclear cells were isolated using Ficoll gradient and Red Blood Cell Lysis. Plasma cells from the bone marrow were isolated using StemCell EasySepEasySep™ Human CD138 Positive SelectionFull Paper
2022White, B;de Reyniès, A;Newman, A;Waterfall, J;Lamb, A;Petitprez, F;Valdeolivas, A;Lin, Y;Li, H;Xiao, X;Wang, S;Zheng, F;Yang, W;Yu, R;Guerrero-Gimenez, M;Catania, C;Lang, B;Domanskyi, S;Bertus, T;Piermarocchi, C;Monaco, G;Caruso, F;Ceccarelli, M;Yu, T;Guo, X;Coller, J;Maecker, H;Duault, C;Shokoohi, V;Patel, S;Liliental, J;Simon, S;Saez-Rodriguez, J;Heiser, L;Guinney, J;Gentles, A;Tumor Deconvolution DREAM Challenge consortium, ;Community assessment of methods to deconvolve cellular composition from bulk gene expressionbioRxivImmune and stromal cells provided by StemExpress and AllCells were isolated according to ) and the rest assigned immune cells from AllCells wherever availability allowed (Tables S5 Save CachedFull Paper
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2022Seenappa, L;Jakubowski, A;Steinbuck, M;Palmer, E;Haqq, C;Carter, C;Fontenot, J;Villinger, F;McNeil, L;DeMuth, P;Programming the lymph node immune response with Amphiphile-CpG induces potent cellular and humoral immunity following COVID-19 subunit vaccination in mice and non-human primatesbioRxivwho had recovered from SARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and ALLCELLS (Alameda, CA). All samples were received and stored frozen at −80°C until analysisFull Paper
2022Lutter, L;ter Linde, J;Brand, E;van Konijnenburg, D;Roosenboom, B;Talabur-Horje, C;Oldenburg, B;van Wijk, F;Compartment-driven imprinting of intestinal CD4 (regulatory) T cells in inflammatory bowel disease and homeostasisbioRxivCluster 1 (18.6% of allcells) was characterized by KLF2, SELL, CCR7, TCF7 and LEF1, and thus resembled recently migrated/recirculating T cells (figure 4A). This cluster contained both intraepithelial and lamina propria T cells (figure 4E). Similarly,Full Paper
2022Jaffe, D;Shahi, P;Adams, B;Chrisman, A;Finnegan, P;Raman, N;Royall, A;Tsai, F;Vollbrecht, T;Reyes, D;McDonnell, W;Functional antibodies exhibit light chain coherencebioRxivWe titrated and developed the B cell fractionation panel using 20 million fresh PBMCs (AllCells, catalog # 3050363) from a healthy human donor whose cells were not used to generate SaveFull Paper
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2022Stuart, T;Hao, S;Zhang, B;Mekerishvili, L;Landau, D;Maniatis, S;Satija, R;Raimondi, I;Nanobody-tethered transposition allows for multifactorial chromatin profiling at single-cell resolutionbioRxivCryopreserved healthy donor PBMCs were isolated from mobilized peripheral blood, BMMCs were purchased from AllCells. After thawing into DMEM with 10% FBS, the cells were spun SaveFull Paper
2022MacMullan, M;Dunn, Z;Qu, Y;Wang, P;Graham, N;Phospho-proteomic analysis of CAR-T cell signaling following activation by antigen-presenting cancer cellsbioRxivcDNAs from BM CD34+, PB CD34+, BM MNCs, PB MNCs and BM MSCs purchased from AllCells (Emeryville, CA, USA) was obtained from a population of positive cells (purity exceeding 95%) collected from five individual donors. The level of cognate cDNA was meaFull Paper
2022Dogan, M;Kozhaya, L;Placek, L;Karabacak, F;Yigit, M;Unutmaz, D;Targeting SARS-CoV-2 infection through CAR-T like bispecific T cell engagers incorporating ACE2bioRxivHealthy adult blood was obtained from AllCells. PBMCs were isolated using Ficoll-paque plus (GE Health care). CD8 T cells were purified using Dynal CD8 Positive Isolation Kit (from SaveFull Paper
2022Yashar, W;Curtiss, B;Coleman, D;Van-Campen, J;Kong, G;Macaraeg, J;Estabrook, J;Demir, E;Long, N;Bottomly, D;McWeeney, S;Tyner, J;Druker, B;Maxson, J;Braun, T;Disruption of the MYC Super-Enhancer Complex by Dual Targeting of FLT3 and LSD1 in Acute Myeloid LeukemiabioRxivWhole bone marrow was obtained (AllCells) and CD34+ cells were selected using CD34 MicroBead Kit (Miltenyi Biotec) according to manufacturer’s instructions. For the colony assay, 500 CD34+ cells were used per replicate and plated in MethoCult™ H4435Full Paper
2022Aksöz, M;Gafencu, G;Stoilova, B;Buono, M;Meng, Y;Jakobsen, N;Metzner, M;Clark, S;Beveridge, R;Thongjuea, S;Vyas, P;Nerlov, C;Identification and Age-dependent Increase of Platelet Biased Human Hematopoietic Stem CellsbioRxivFresh BM from healthy young volunteers (21 to 34 years old males) were purchased from AllCells (Berkeley, CA) or Lonza (Lonza Bioscience, Basel, Switzerland). Bone marrow from older donors was obtained from orthoplastic surgery afterFull Paper
2022Xiang Ping, M;Zhi, H;Aziz, N;Hadri, N;Ghazalli, N;Yusop, N;Optimization of agarose-alginate hydrogel bead components for encapsulation and transportation of stem cellsJournal of Taibah University Medical SciencesStem cells from human exfoliated deciduous teeth (SHED) purchased from AllCells USA were revived from the cryovial and maintained in culture before encapsulation. SHED from SaveFull Paper
2022Marcisak, EF;Leveraging single-cell genomics to uncover clinical and preclinical responses to cancer immunotherapyThesisGFP due to transduction with pBMN-IRES-EGFP containing the FcγRIIIA construct. All NK cell lines were cultured as previously described[231]. Fresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selectedFull Paper
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2022Chu, J;Ligament tissue engineering: assessing differentiation potential of dental follicle cells in a self-assembly three-dimensional organoid model.Thesisaccording to AllCells company regulations, further donor information such gender and exact age was not provided to the purchaser). The PDL-hTERT immortal cell line has already SaveFull Paper
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2022BOSYKH, D;Mechanisms and Biological Consequences of Mutual Reprogramming by Melanoma Cells and Normal Dermal FibroblastsThesisCell lines and medium Primary human neonatal dermal fibroblasts (NDFs; AllCells, LLC) were combined from three individual donors. NDFs were routinely tested for mycoplasma and confirmed to be negative using the MycoAlert mycoplasma detection kit (LonFull Paper
2022Oostindie, SC;Rinaldi, DA;Zom, GG;Wester, MJ;Paulet, D;Al-Tamimi, K;van der Meijden, E;Scheick, JR;Wilpshaar, T;de Jong, B;Hoff-van den Broek, M;Grattan, RM;Oosterhoff, JJ;Vignau, J;Verploegen, S;Boross, P;Beurskens, FJ;Lidke, DS;Schuurman, J;de Jong, RN;Logic-gated antibody pairs that selectively act on cells co-expressing two antigensNature biotechnology35879362PBMCs derived from patients with CLL were commercially obtained from Discovery Life Sciences. Characteristics of patients with CLL are summarized in Supplementary SaveFull Paper
2022Schuler, M;Cuppens, K;Ploenes, T;Vanbockrijck, M;Wiesweg, M;Darwiche, K;Schramm, A;Maes, B;Hegedus, B;Schildhaus, H;Hautzel, H;Theegarten, D;Baas, P;Hartemink, K;Du Pont, B;Aigner, C;LBA37 A randomized, multicentric phase II study of preoperative nivolumab plus relatlimab or nivolumab in patients with resectable non-small cell lung cancer (NEOpredict-Lung)Annals of Oncologycology, AstraZeneca, Eisai, Takeda, ZytoVision, Zytomed Systems, Molecular Health; Financial Interests, Institutional, Research Grant: Novartis Oncology; Financial Interests, Personal, Full or part-time Employment: Targos Molecular Pathology Inc.. H.Full Paper
2022Baraibar Argota, I;Garcia Rodriguez, A;Salvà Ballabrera, F;Ros Montana, F;Saoudi Gonzalez, N;Comas, R;Castillo, G;Sanchis, M;Hernando Cubero, J;García-Alvarez, A;Capdevila Castillon, J;Martí, M;Landolfi, S;Espin, E;Nuciforo, P;Vivancos, A;Tabernero, J;Elez Fernandez, M;332P Impact of the COVID-19 pandemic in the early-onset colorectal cancer (EOCRC)Annals of Oncologyersonal, Invited Speaker: Novartis; Financial Interests, Personal, Advisory Board: MSD Oncology, Bayer; Financial Interests, Personal, Other, Consultant: Targos Molecular Pathology GmbH. A. Vivancos: Financial Interests, Personal, Advisory Board: RocFull Paper
2022Lau, D;Khare, S;Stein, MM;Jain, P;Gao, Y;BenTaieb, A;Rand, TA;Salahudeen, AA;Khan, AA;Integration of tumor extrinsic and intrinsic features associates with immunotherapy response in non-small cell lung cancerNature communications35831288Samples for single-cell multi-omic sequencing were previously frozen dissociated tumor cells (DTCs) (Discovery Life Sciences, Huntsville, AL). DTCs were thawed and Save;xpression analyses were performed on log10-transformed counts. Single-cell mulFull Paper
2022Leung, DH;Devaraj, S;Goodrich, NP;Chen, X;Rajapakshe, D;Ye, W;Andreev, V;Minard, CG;Guffey, D;Molleston, JP;Bass, LM;Karpen, SJ;Kamath, BM;Wang, KS;Sundaram, SS;Rosenthal, P;McKiernan, P;Loomes, KM;Jensen, MK;Horslen, S;Bezerra, JA;Magee, JC;Merion, RM;Sokol, RJ;Shneider, BL;For ChiLDReN, ;Serum Biomarkers Correlated with Liver Stiffness Assessed in a Multi-center Study of Pediatric Cholestatic Liver DiseaseHepatology (Baltimore, Md.)36069569similar distributions of age- and sex-matched children with normal liver biochemistries and without known liver disease served as case controls (Discovery Life Sciences, SaveFull Paper
2022Yap, TA;Gainor, JF;Callahan, MK;Falchook, GS;Pachynski, RK;LoRusso, P;Kummar, S;Gibney, GT;Burris, HA;Tykodi, SS;Rahma, OE;Seiwert, TY;Papadopoulos, KP;Blum Murphy, M;Park, H;Hanson, A;Hashambhoy-Ramsay, Y;McGrath, L;Hooper, E;Xiao, X;Cohen, H;Fan, M;Felitsky, D;Hart, C;McComb, R;Brown, K;Sepahi, A;Jimenez, J;Zhang, W;Baeck, J;Laken, H;Murray, R;Trehu, E;Harvey, CJ;First-in-Human Phase I/II ICONIC Trial of the ICOS Agonist Vopratelimab Alone and with Nivolumab: ICOS-High CD4 T-Cell Populations and Predictors of ResponseClinical cancer research : an official journal of the American Association for Cancer Research35511938Full Paper
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2022Koehler, VF;Adam, P;Fuss, CT;Jiang, L;Berg, E;Frank-Raue, K;Raue, F;Hoster, E;Knösel, T;Schildhaus, HU;Negele, T;Siebolts, U;Lorenz, K;Allelein, S;Schott, M;Spitzweg, C;Kroiss, M;German Study Group for Rare Malignant Tumors of the Thyroid and Parathyroid Glands, ;Treatment of RET-Positive Advanced Medullary Thyroid Cancer with Multi-Tyrosine Kinase Inhibitors-A Retrospective Multi-Center Registry AnalysisCancers35884466US is an employee of Targos Molecular Pathology, Inc., and received honoraria from or served as an advisory board member at Roche, Novartis Oncology, MSD, BMS, Save CachedFull Paper
2022Molina, SA;Davies, SJ;Sethi, D;Oh, S;Durand, N;Scott, M;Davies, LC;Wormuth, K;Clarke, D;Particulates are everywhere, but are they harmful in cell and gene therapies?Cytotherapy36175323International Society for Cell & Gene Therapy Process Development, Manufacturing, and Analytics Committee, Vancouver, Canada. Electronic address: sam.molina@gmail.com.++International Society for Cell & Gene Therapy Process Development, Manufacturing,Full Paper
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2022Suwa, R;Kume, Y;Kawase, M;Chishiki, M;Ono, T;Norito, S;Sato, K;Okamoto, M;Kumaki, S;Nagai, Y;Hosoya, M;Takeda, M;Nishimura, H;Hashimoto, K;Shirato, K;Practical Validation of United States Centers for Disease Control and Prevention Assays for the Detection of Human Respiratory Syncytial Virus in Pediatric Inpatients in JapanPathogens (Basel, Switzerland)35889999To confirm amplification specificity, we used pharyngeal, nasopharyngeal, and nasal swabs from Discovery Life Sciences (Los Osos, CA, USA) that were obtained for the Save CachedFull Paper
2022Rodrigues, D;van Kampen, R;van Bodegraven, AA;Kleinjans, JCS;Jennen, DGJ;de Kok, TM;Gene expression responses reflecting 5-FU-induced toxicity: Comparison between patient colon tissue and 3D human colon organoidsToxicology letters36183961All patients provided written informed consent. Human colon tissue samples were obtained by Boehringer Ingelheim Pharmaceuticals Inc. (Ridgefield, Connecticut, USA) from Discovery Life Sciences (Huntsville, Alabama, USA; formerly, Conversant BiologicFull Paper
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2022Holmberg, JA;Henry, SM;Burnouf, T;Devine, D;Marschner, S;Boothby, TC;Burger, SR;Chou, ST;Custer, B;Blumberg, N;Siegel, DL;Spitalnik, SL;National Blood Foundation 2021 Research and Development summit: Discovery, innovation, and challenges in advancing blood and biotherapiesTransfusion36169155consulting firm, and one of our clients is Discovery Life Sciences, an, apheresis services provider. My advice to DLS is the same as what I say in this manuscript. HemaCare SaveFull Paper
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2022Hebda-Bauer, E;Hagenauer, M;Blandino, P;Meng, F;Chitre, A;Ozel, A;Arakawa, K;Flagel, S;Watson, S;Palmer, A;Li, J;Akil, H;Transcriptional Profiling of the Hippocampus in an F2 Cross of a Genetic Rat Model of Internalizing vs. Externalizing Behavior and Addiction LiabilitybioRxivHudsonAlpha Discovery provided a variety of metrics for evaluating sample RNA quality, including RNA concentration (RNAconc), RNA integrity number (RIN), the Save CachedFull Paper
2022Chitre, A;Hebda-Bauer, E;Blandino, P;Bimschleger, H;Nguyen, K;Maras, P;Li, F;Ozel, A;Polysskaya, O;Cheng, R;Flagel, S;Watson, S;Li, J;Akil, H;Palmer, A;Genome-Wide Association Study in a Rat Model of Temperament Identifies Multiple Loci for Exploratory Locomotion and Anxiety-Like TraitsbioRxivFor these 20 rats, liver tissue was sent to HudsonAlpha Discovery (Discovery Life Sciences) for HMW genomic DNA extraction and long-range and deep sequencing. SaveFull Paper
2022Dejanovic, B;Wu, T;Tsai, M;Graykowski, D;Gandham, V;Rose, C;Bakalarski, C;Ngu, H;Wang, Y;Pandey, S;Rezzonico, M;Friedman, B;Edmonds, R;De Mazière, A;Rakosi-Schmidt, R;Singh, T;Klumperman, J;Foreman, O;Chang, M;Xie, L;Sheng, M;Hanson, J;Complement C1q-dependent excitatory and inhibitory synapse elimination by astrocytes and microglia in Alzheimer’s disease mouse modelsNature AgingCSF from patients with AD and healthy controls was obtained from Folio Biosciences and Precision Medicine with ethics committee approval and written informed consentFull Paper
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2022Newman, L;Useckaite, Z;Rowland, A;Addressing MISEV guidance using targeted LC?MS/MS: A method for the detection and quantification of extracellular vesicle?enriched and contaminant protein markers from bloodJournal of Extracellular BiologySerum and plasma samples from patients with non-alcoholic fatty liver disease (NAFLD) were purchased from Discovery Life Sciences (Hunstville, AL, USA). SaveFull Paper
2022D'ANTONIO, L;Esposizione a paracetamolo e acido sulfosalicilico: effetti biologici nel bivalve Mytilus spp.ThesisFrancisca Piedade, Sofia Bio, Bruno Nunes. 2020 Effects of common pharmaceutical drugsFull Paper
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2022Ferreira Garcia Rodrigues, D;Gut feelingsmaastricht universityHuman healthy colon organoids were derived from a tissue biopsy collected from a healthy male donor (67 years old) and kindly provided by Boehringer Ingelheim Pharmaceuticals Inc. (Ridgefield, USA), who purchased tissue biopsies from Discovery Life SFull Paper
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2022Shen, Y;Guo, K;Ma, A;Huang, Z;Du, J;Chen, J;Lin, Q;Wei, C;Wang, Z;Zhang, F;Zhang, J;Lin, W;Feng, N;Ma, W;Mitochondrial toxicity evaluation of traditional Chinese medicine injections with a dual in vitro approachFrontiers in pharmacology36408232and Health Sciences, Wuyi University, Jiangmen, China 3 Antibody Engineering Laboratory, School of Life Science & Technology, China Pharmaceutical University, Nanjing, China Edited by: Albert P. Li, In Vitro ADMET Laboratories, United States ReviewFull Paper
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2022Drapkin, R;Jung, E;Bradshaw, C;Demars, L;Mosher, R;EP014/#408?Evaluation of NAPI2B expression in a well annotated longitudinal tissue series of ovarian serous carcinomasE-PostersIHC analyses were performed by QualTek Molecular Laboratories (Discovery Life Sciences). Editorial support for this poster was provided by BluPrint Oncology. SaveFull Paper
2022Korner, LK;Cell death and inflammation in skin homeostasis and small cell lung cancerThesisR.B. is an employee of Targos Molecular Pathology. The other authors declare no competing interestsFull Paper
2022Weng, H;Huang, F;Yu, Z;Chen, Z;Prince, E;Kang, Y;Zhou, K;Li, W;Hu, J;Fu, C;Aziz, T;Li, H;Li, J;Yang, Y;Han, L;Zhang, S;Ma, Y;Sun, M;Wu, H;Zhang, Z;Wunderlich, M;Robinson, S;Braas, D;Hoeve, JT;Zhang, B;Marcucci, G;Mulloy, JC;Zhou, K;Tao, HF;Deng, X;Horne, D;Wei, M;Huang, H;Chen, J;The m6A reader IGF2BP2 regulates glutamine metabolism and represents a therapeutic target in acute myeloid leukemiaCancer cell36306790Bone marrow (BM) cells were collected from 5- to 7-week-old wild-type, Mettl14fl/flCreERT, or Igf2bp2fl/fl mice, and BM progenitor (HSPC, i.e., Lin- or c-kit+) cells were enriched with the Mouse Lineage Cell Depletion Kit or the CD117 microbeads (MilFull Paper
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2022Devoucoux, M;Fort, V;Khelifi, G;Xu, J;Alerasool, N;Galloy, M;Wong, N;Bourriquen, G;Fradet-Turcotte, A;Taipale, M;Hope, K;Hussein, SMI;Côté, J;Oncogenic ZMYND11-MBTD1 fusion protein anchors the NuA4/TIP60 histone acetyltransferase complex to the coding region of active genesCell reports35705031Recombinant Mouse TPO PeproTech Cat# AF-315-14 Recombinant Mouse IL-3 R&D Systems Cat# 403-ML-050 Recombinant Mouse IL-6 R&D Systems Cat# 406-ML-025 ColonyGEL, Mouse Complete Medium ReachBio Cat# 1202 SFEM II Media STEMCELL Technologies Cat# 09655Full Paper
2022Peterson, CW;Venkataraman, R;Reddy, SS;Pande, D;Enstrom, MR;Radtke, S;Humbert, O;Kiem, HP;Intracellular RNase activity dampens zinc finger nuclease-mediated gene editing in hematopoietic stem and progenitor cellsMolecular therapy. Methods & clinical development34977270nces, San José, CA) into D-PBS, followed by total genomic DNA extraction and NGS. Colony-forming assays ZFN- or CRISPR/Cas9-treated NHP cells (1.2 × 103) were plated in 3.6 mL of ColonyGEL 1402 (ReachBio, Seattle, WA). For colony-forming assays utilFull Paper
2022Liu, L;Li, H;Patterson, AM;Plett, PA;Sampson, CH;Mohammad, KS;Capitano, ML;Singh, P;Yao, C;Orschell, CM;Pelus, LM;Upregulation of SIRT1 Contributes to dmPGE2-dependent Radioprotection of Hematopoietic Stem CellsStem cell reviews and reports35318613Colony forming cells were analyzed as described [20]. Total nucleated BM cells from non-irradiated mice were plated at 2 × 104 per dish in Mouse Complete Colony Gel media (Reach Bio, Seattle, WA). BM cells from irradiated C57BL/6 mice were plated atFull Paper
2022Canals Hernaez, D;Hughes, MR;Li, Y;Mainero Rocca, I;Dean, P;Brassard, J;Bell, EM;Samudio, I;Mes-Masson, AM;Narimatsu, Y;Clausen, H;Blixt, O;Roskelley, CD;McNagny, KM;Targeting a Tumor-Specific Epitope on Podocalyxin Increases Survival in Human Tumor Preclinical ModelsFrontiers in oncology35600398Linker stability was independently assessed by ReachBio Research Labs (RRL, www.reachbio.com) using an in vitro neutrophil differentiation killing assay, where extracellular Save;Emer Clarke at ReachBio Research Labs (www.reachbio.com) for providinFull Paper
2022Lefavor, R;Clarke, E;The Effect of Various Stimulants on Cytokine Secretion Profiles in Freshly Isolated Peripheral Blood Mononuclear Cells (PBMC)The Journal of Immunology1ReachBio Research Labs++1ReachBio Research LabsFull Paper
2022Gu, Q;Palani, CD;Smith, A;Li, B;Amos-Abanyie, EK;Ogu, U;Lu, L;Pace, BS;Starlard-Davenport, A;MicroRNA29B induces fetal hemoglobin via inhibition of the HBG repressor protein MYB in vitro and in humanized sickle cell miceFrontiers in medicine36507536kville, MD, USA) was used alone or co-transfected with MIR29B. Erythroid differentiation of human CD34+ stem cells and microRNA29B co-electroporation with MYB DNA Human bone marrow CD34+ stem cells (ReachBio, Seattle, WA, USA) were cultured in a modFull Paper
2022Balk, R;Esper, A;Martin, G;Miller, R;Lopansri, B;Burke, J;Levy, M;Opal, S;Rothman, R;D'Alessio, F;Sidhaye, V;Aggarwal, N;Greenberg, J;Yoder, M;Patel, G;Gilbert, E;Parada, J;Afshar, M;Kempker, J;van der Poll, T;Schultz, M;Scicluna, B;Klein Klouwenberg, P;Liebler, J;Blodget, E;Kumar, S;Navalkar, K;Yager, T;Sampson, D;Kirk, J;Cermelli, S;Davis, R;Brandon, R;Validation of SeptiCyte RAPID to discriminate sepsis from non-infectious systemic inflammationmedRxivThe authors thank the clinical study coordinators of the NEPTUNE study: Joyce D. Brown (Rush University), Liliacna Jara (University of Southern California), Leona Wells and Maya C. Whaley (Grady Memorial Hospital). The authors also thank the laboratoFull Paper
2022Vujovic, A;de Rooij, L;Chahi, A;Chen, H;Yee, B;Loganathan, S;Liu, L;Chan, D;Tajik, A;Tsao, E;Moreira, S;Joshi, P;Xu, J;Wong, N;Zandi, S;Aigner, S;Dick, J;Minden, M;Schramek, D;Yeo, G;Hope, K;A two-step in vivo CRISPR screen unveils pervasive RNA binding protein dependencies for leukemic stem cells and identifies ELAVL1 as a therapeutic targetbioRxivThawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio), supplemented with DMSO or 1.1uM DHTS. Colonies were scored on days 10-14. Human CB samples wereFull Paper
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2021Schneider, BP;Jiang, G;Ballinger, TJ;Shen, F;Chitambar, C;Nanda, R;Falkson, C;Lynce, FC;Gallagher, C;Isaacs, C;Blaya, M;Paplomata, E;Walling, R;Daily, K;Mahtani, R;Thompson, MA;Graham, R;Cooper, ME;Pavlick, DC;Albacker, LA;Gregg, J;Solzak, JP;Chen, YH;Bales, CL;Cantor, E;Hancock, BA;Kassem, N;Helft, P;O'Neil, B;Storniolo, AMV;Badve, S;Miller, KD;Radovich, M;BRE12-158: A Postneoadjuvant, Randomized Phase II Trial of Personalized Therapy Versus Treatment of Physician's Choice for Patients With Residual Triple-Negative Breast CancerJournal of clinical oncology : official journal of the American Society of Clinical Oncology34910554Speakers' Bureau: Genomic Health, Targos Molecular Pathology, AgilentFull Paper
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2021Binnewies, M;Pollack, JL;Rudolph, J;Dash, S;Abushawish, M;Lee, T;Jahchan, NS;Canaday, P;Lu, E;Norng, M;Mankikar, S;Liu, VM;Du, X;Chen, A;Mehta, R;Palmer, R;Juric, V;Liang, L;Baker, KP;Reyno, L;Krummel, MF;Streuli, M;Sriram, V;Targeting TREM2 on tumor-associated macrophages enhances immunotherapyCell reports34686340Human tumor samples used for both flow cytometry and single-cell RNA sequencing were acquired from Discovery Life Sciences. 49 human tumor samples were used for flow cytometric analysis of immune composition and TREM2 surface levels. 10 human tumorFull Paper
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2021Israeli, S;Golden, A;Atalig, M;Mekki, N;Rais, A;Storey, H;Barbouche, MR;Peck, R;A Novel Point-of-Care Rapid Diagnostic Test for Screening Individuals for Antibody DeficienciesJournal of clinical immunology34839430These thirty-three specimens were acquired from Discovery Life Sciences (Los Osos, CA, USA) with IgG concentration characterized by Siemens BN II System nephelometer (Siemens Healthcare GmbH, Erlangen, Germany). IgG levels in the chosen samples SaFull Paper
2021Cheng, Y;Hall, TR;Xu, X;Yung, I;Souza, D;Zheng, J;Schiele, F;Hoffmann, M;Mbow, ML;Garnett, JP;Li, J;Targeting uPA-uPAR interaction to improve intestinal epithelial barrier integrity in inflammatory bowel diseaseEBioMedicine34933179anoids isolation and culture Isolation and culture of primary intestinal organoids was performed as previously described.2223 Briefly, human small intestinal tissues (obtained from Coversant Bio, now Discovery Life Sciences, https://www.dls.com/biospFull Paper
2021Cheng, L;Creasy, T;Pilataxi, F;Greenlees, L;Vence, L;Sridhar, S;Streicher, K;Effects of combination treatment with durvalumab plus tremelimumab on the tumor microenvironment in non-small-cell lung carcinomaCancer immunology, immunotherapy : CII34623465Frozen NSCLC tumor digests (untreated patients, three squamous cell carcinomas and one adenocarcinoma) were purchased from Discovery Life Sciences and cultured in 96-well plates in a medium containing low-dose interleukin 2 (IL-2) plus 20 µg/mL D aFull Paper
2021Bröske, AE;Korfi, K;Belousov, A;Wilson, S;Ooi, CH;Bolen, CR;Canamero, M;Alcaide, EG;James, I;Piccione, EC;Carlile, DJ;Dimier, N;Umaña, P;Bacac, M;Weisser, M;Dickinson, M;Pharmacodynamics and molecular correlates of response to glofitamab in relapsed/refractory non-Hodgkin lymphomaBlood advances34941996Biopsies were sent to the central pathology laboratory (Targos Molecular Pathology GmbH, Kassel, Germany) and fresh tissues were embedded on receipt. All biopsies were quality checked by a pathologist on hematoxylin and eosin (H&E) stainingFull Paper
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2021Manos, J;Preiss, C;Venkat, N;Tamm, J;Reinhardt, P;Kwon, T;Wu, J;Winter, A;Jahn, T;Yanamandra, K;Titterton, K;Karran, E;Langlois, X;UNCOVERING SPECIFICITY OF ENDOGENOUS TAU AGGREGATION IN A HUMAN IPSC-NEURON TAU SEEDING MODELiScience(Braak stage V/VI) and non-AD (Braak stage 0/I) male and female donors used in preparing sarkosyl-insoluble tau seeds were generously provided by the Massachusetts Alzheimer's Disease Research Center or purchased from Folio (now Discovery Life ScieFull Paper
2021Da Mesquita, S;Papadopoulos, Z;Dykstra, T;Brase, L;Farias, FG;Wall, M;Jiang, H;Kodira, CD;de Lima, KA;Herz, J;Louveau, A;Goldman, DH;Salvador, AF;Onengut-Gumuscu, S;Farber, E;Dabhi, N;Kennedy, T;Milam, MG;Baker, W;Smirnov, I;Rich, SS;Dominantly Inherited Alzheimer Network, ;Benitez, BA;Karch, CM;Perrin, RJ;Farlow, M;Chhatwal, JP;Holtzman, DM;Cruchaga, C;Harari, O;Kipnis, J;Meningeal lymphatics affect microglia responses and anti-Aβ immunotherapyNature33911285FC-404-2001) cartridges. Processing of total RNA extracted from mouse meningeal LECs (including linear RNA amplification and cDNA library generation) and RNA-seq was performed by HudsonAlpha Genomic Services Laboratory. For bulk RNA-seq experiments wFull Paper
2021Matusz-Fisher, A;Tan, AR;Combination of HER2-targeted agents with immune checkpoint inhibitors in the treatment of HER2-positive breast cancerExpert opinion on biological therapy34806498PD-L1-positivity was defined as staining in ≥ 1% of tumor cells or any staining in the stroma using the QualTek immunohistochemistry (IHC) assay. Of note, the assay was changed to the Dako IHC 22C3 pharmDx Q 2 Solutions assay mid-trial due to the pFull Paper
2021Luk, KC;Gersch, J;Harris, BJ;Holzmayer, V;Mbanya, D;Sauleda, S;Rodgers, MA;Cloherty, G;More DNA and RNA of HBV SP1 splice variants are detected in genotypes B and C at low viral replicationScientific reports34903774genotyping through the Abbott Global Surveillance Program38. All other plasma specimens sourced from France, Germany, Italy, Spain and Vietnam with known viral loads and genotypes were purchased from Discovery Life Sciences (Huntsville, AL). HBV DNAFull Paper
2021Connolly, P;Stapleton, S;Mosoyan, G;Fligelman, I;Tonar, YC;Fleming, F;Donovan, MJ;Analytical validation of a multi-biomarker algorithmic test for prediction of progressive kidney function decline in patients with early-stage kidney diseaseClinical proteomics34789168one specimens collected from patients receiving treatment with biologic therapeutic TNF-α inhibitors used to treat rheumatoid and psoriatic arthritis and other auto-immune diseases were sourced from Discovery Life Sciences (Huntsville, AL). SpecimensFull Paper
2021Jöhrens, K;Rüschoff, J;The Challenge to the Pathologist of PD-L1 Expression in Tumor Cells of Non-Small-Cell Lung Cancer-An OverviewCurrent oncology (Toronto, Ont.)34940076Institute of Pathology, Carl Gustav Carus University Hospital Dresden, 01307 Dresden, Germany.++Targos Molecular Pathology GmbH, 34119 Kassel, GermanyFull Paper
2021Hou, X;Wang, G;Fan, W;Chen, X;Mo, C;Wang, Y;Gong, W;Wen, X;Chen, H;He, D;Mo, L;Jiang, S;Ou, M;Guo, H;Liu, H;T-cell receptor repertoires as potential diagnostic markers for patients with COVID-19International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases34688948ad recovered from COVID-19. In the COVID-19-BWNW group, whole blood samples were collected at Bloodworks Northwest (Seattle, WA, USA). In the COVID-19-DLS group, whole blood samples were collected at Discovery Life Sciences (Huntsville, AL, USA). InFull Paper
2021Brosius, C;Caron, K;Sosnoff, C;Blount, B;Wang, L;Rapid Development and Validation of a Liquid Chromatography-Tandem Mass Spectrometry Method to Measure Cannabinoids in Bronchoalveolar-Lavage Fluid of Patients with e-Cigarette, or Vaping, Product Use-Associated Lung InjuryACS OmegaBAL pools were created using anonymous BAL samples acquired commercially from Discovery Life Sciences (Huntsville, AL). They were shipped frozen on dry ice and then stored in −70 C freezers until analyzed. Ten individual BAL fluids were screened foFull Paper
2021Sun, Q;Pastor, L;Du, J;Powell, MJ;Zhang, A;Bodmer, W;Wu, J;Zheng, S;Sha, MY;A novel xenonucleic acid-mediated molecular clamping technology for early colorectal cancer screeningPloS one34610014give an average DNA fragment length of about 150 bp, which mimics the size of cfDNA fragments, justifying their use as cfDNA references. Except for the 10 CRC whole blood samples were purchased from Discovery Life Sciences (Huntsville, AL, US), AllFull Paper
2021Pound, H;Gann, E;Wilhelm, S;A comparative study of metatranscriptomic assessment methods to characterize Microcystis bloomsLimnology and Oceanography: MethodsExtracted RNA was processed using a Illumina Stranded Total RNA Prep, Ligation with Ribo-Zero Plus and then 50-million 100-bp paired-end reads were generated on the Illumina NovaSeq platform at Hudson Alpha Discovery Life Sciences (Huntsville, SaFull Paper
2021Miao, LY;Kim, HJ;Whitlatch, K;Jaiswal, D;Navarro, A;Egan, R;Olivo, PD;A rapid homogenous bioassay for detection of thyroid-stimulating antibodies based on a luminescent cyclic AMP biosensorJournal of immunological methods34871593TSI-positive serum samples were a kind gift of Dr. George Kahaly. Anti-thyroid peroxidase (TPO) antibody-positive human serum samples and nonthyroidal autoimmune disease state human serum samples were obtained from Discovery Life Science (Los Osos, CFull Paper
2021Nuovo, G;Suster, D;Tili, E;Awad, H;Magro, C;A Standardization Protocol for the In Situ Detection of SARS-CoV2 RNA and ProteinsApplied Immunohistochemistry & Molecular Morphologyvid MD‡; Tili, Esmerina PhD§; Awad, Hamdy MD§; Magro, Cynthia MD∥ Author Information __ *Ohio State University Comprehensive Cancer Center §Ohio State University Medical Center, Columbus †Discovery Life Sciences, Powell, OH ‡Department of PatholoFull Paper
2021Nguyen, M;Gary, J;Sonoda, J;Low, M;Ring, C;Bishop, J;Feasibility of Lateral Flow Assays for Urinary Biomarkers to Nicotine Delivery ProductsOSF PREPRINTS2021A prospective collection of 212 individual urine specimens from users and non-nicotine users was completed between July 2020 and April 2021 by Discovery Life Sciences (Los Osos, CA). All enrollees selfidentified as non-users (112 samples) or singleFull Paper
2021Zhang, L;Pohl, C;Homan, K;Sun, V;Gaudette, S;Nelson, C;Erickson, J;Knight, H;Bose, S;Lynch, G;McRae, B;Sterman, A;Mitra, S;Imaging the Alternatively Spliced D Domain of Tenascin C in Preclinical Models of Inflammatory Bowel DiseaseResearch SquareClinical IBD and diseased cynomolgus macaque colon were evaluated using a biotinylated anti-TNC D antibody. Frozen human tissue was obtained from CHTN (National Cancer Institute, Bethesda, MD), Folio Biosciences (Columbus, OH), and UMASS Memorial GasFull Paper
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2021Hair, J;Robinson, MJ;Wilkinson, RW;Dovedi, SJ;Deep phenotyping of surface stimulatory and inhibitory co-receptors on cancer-resident T and NK cells reveals cell subsets within the tumor-reactive CTL population that are uniquely defined by NKG2A expressionSLAS DiscoveryDisaggregated tumor cells (DTC; Discovery Life Sciences), which are a single-cell suspension of enzymatically-dissociated tumor tissue, from NSCLC (n=13), RC (n=10) and melanoma (n=10) patients were used. Detailed per-patient disease classificationFull Paper
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2021Valle, J;Kelley, R;Nervi, B;Oh, D;Zhu, A;Biliary tract cancerThe LancetIn KEYNOTE-028 PD-L1 positivity was defined as membranous PD-L1 expression in at least 1% of tumour and associated inflammatory cells, or positive staining in stroma (immunohistochemistry assay, QualTek, Goleta, CA, USA), and all patients were PD-L1Full Paper
2021Tiu, C;Derby, S;Haris, N;Welsh, L;Stansfeld, A;Hundsberger, T;Roth, P;König, F;Eisner, J;Kleinschmidt, M;Anderson, S;Bachmann, F;Lane, H;Engelhardt, M;Kaindl, T;Litherland, K;Stan, A;Evans, T;Plummer, E;Lopez, J;The potential utility of end-binding protein 1 (EB1) as response-predictive biomarker for lisavanbulin: A phase 2 study of lisavanbulin (BAL101553) in adult patients with recurrent glioblastoma.Journal of Clinical OncologyGallen, Switzerland; Department of Neurology, University Hospital Zurich, Zurich, Switzerland; Targos Molecular Pathology GmbH, Kassel, Germany; GeneCentric Therapeutics, Inc., Research Triangle Park, NC; Basilea Pharmaceutica International Ltd., BasFull Paper
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2021Pratto, F;Brick, K;Cheng, G;Lam, KG;Cloutier, JM;Dahiya, D;Wellard, SR;Jordan, PW;Camerini-Otero, RD;Meiotic recombination mirrors patterns of germline replication in mice and humansCell34260899Human testicular biopsy from cancer patient (Normal adjacent tissue) Folio Biosciences now Discovery Life Sciences biospecimen bank https://www.dls.comFull Paper
2021Scoazec, J;Bauchet, A;Adam, J;Rochaix, P;MacGrogan, G;Kim, J;Nuciforo, P;López-Rios, F;Frisman, D;Cesarone, G;Bernstein, S;Lee, J;Henry, C;Ozoux, M;Lefebvre, A;1313P Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) tumor expression in a phase I/II study of tusamitamab ravtansine (SAR408701) in patients (pts) with advanced non-small cell lung cancer (NSCLC)Annals of Oncologyarticles by this author. D. Frisman D. Frisman. Affiliations Discovery Life Sciences, Goleta, CA, USA. Search for articles by this author. G. Cesarone G. Cesarone. Affiliations Discovery Life Sciences, Goleta, CA, USA. Search for articlesFull Paper
2021Salgado, R;Peg, V;Rüschoff, J;Vincent-Salomon, A;Castellano, I;Perner, S;Van de Vijver, K;Quinn, CM;Varga, Z;Gene expression signatures for tailoring adjuvant chemotherapy of luminal breast cancer: the pathologists' perspectiveAnnals of oncology : official journal of the European Society for Medical Oncology34461263Department of Pathology, GZA-ZNA Hospitals, Antwerp, Belgium; Division of Research, Peter MacCallum Cancer Centre, Melbourne, Australia. Electronic address: roberto@salgado.be.++Universidad Autónoma de Barcelona, Barcelona, Spain; Department of PathoFull Paper
2021Keenan, TE;Guerriero, JL;Barroso-Sousa, R;Li, T;O'Meara, T;Giobbie-Hurder, A;Tayob, N;Hu, J;Severgnini, M;Agudo, J;Vaz-Luis, I;Anderson, L;Attaya, V;Park, J;Conway, J;He, MX;Reardon, B;Shannon, E;Wulf, G;Spring, LM;Jeselsohn, R;Krop, I;Lin, NU;Partridge, A;Winer, EP;Mittendorf, EA;Liu, D;Van Allen, EM;Tolaney, SM;Molecular correlates of response to eribulin and pembrolizumab in hormone receptor-positive metastatic breast cancerNature communications34548479Tumors from 65/88 (74%) patients underwent PD-L1 testing assessed centrally by QualTek using the 22C3 antibodyFull Paper
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2021Ny, L;Jespersen, H;Karlsson, J;Alsén, S;Filges, S;All-Eriksson, C;Andersson, B;Carneiro, A;Helgadottir, H;Levin, M;Ljuslinder, I;Olofsson Bagge, R;Sah, VR;Stierner, U;Ståhlberg, A;Ullenhag, G;Nilsson, LM;Nilsson, JA;The PEMDAC phase 2 study of pembrolizumab and entinostat in patients with metastatic uveal melanomaNature communications34453044st was used to assess associations between immunohistochemical analyses (PD-L1 and TILs) and clinical benefit. Immunohistochemical analyses Tumor PD-L1 testing was performed at a central laboratory (QualTek Molecular Laboratories, Newtown, PennsylvaFull Paper
2021Cindy Yang, SY;Lien, SC;Wang, BX;Clouthier, DL;Hanna, Y;Cirlan, I;Zhu, K;Bruce, JP;El Ghamrasni, S;Iafolla, MAJ;Oliva, M;Hansen, AR;Spreafico, A;Bedard, PL;Lheureux, S;Razak, A;Speers, V;Berman, HK;Aleshin, A;Haibe-Kains, B;Brooks, DG;McGaha, TL;Butler, MO;Bratman, SV;Ohashi, PS;Siu, LL;Pugh, TJ;Pan-cancer analysis of longitudinal metastatic tumors reveals genomic alterations and immune landscape dynamics associated with pembrolizumab sensitivityNature communications34446728ary Fig. 9A. PD-L1 immunohistochemistry Immunohistochemical (IHC) staining for PD-L1 using the mouse monoclonal anti-PD-L1 antibody (clone 22C3 at 2 μg/mL, Merck, Palo Alto, CA) was performed by Qualtek Molecular Laboratories (Newtown, PA, USA). TheFull Paper
2021Fowler, TW;Mitchell, TL;Janda, CY;Xie, L;Tu, S;Chen, H;Zhang, H;Ye, J;Ouyang, B;Yuan, TZ;Lee, SJ;Newman, M;Tripuraneni, N;Rego, ES;Mutha, D;Dilip, A;Vuppalapaty, M;Baribault, H;Yeh, WC;Li, Y;Development of selective bispecific Wnt mimetics for bone loss and repairNature communications34059688The following method was used for human and mouse femur bone (Fig. 1d, e). Fresh human cadaveric femur tissue was purchased from vendor (Folio Conversant). Femur heads were shipped on wet ice within 24 h of death and tissue used immediately upon receFull Paper
2021Butler, D;Mozsary, C;Meydan, C;Foox, J;Rosiene, J;Shaiber, A;Danko, D;Afshinnekoo, E;MacKay, M;Sedlazeck, FJ;Ivanov, NA;Sierra, M;Pohle, D;Zietz, M;Gisladottir, U;Ramlall, V;Sholle, ET;Schenck, EJ;Westover, CD;Hassan, C;Ryon, K;Young, B;Bhattacharya, C;Ng, DL;Granados, AC;Santos, YA;Servellita, V;Federman, S;Ruggiero, P;Fungtammasan, A;Chin, CS;Pearson, NM;Langhorst, BW;Tanner, NA;Kim, Y;Reeves, JW;Hether, TD;Warren, SE;Bailey, M;Gawrys, J;Meleshko, D;Xu, D;Couto-Rodriguez, M;Nagy-Szakal, D;Barrows, J;Wells, H;O'Hara, NB;Rosenfeld, JA;Chen, Y;Steel, PAD;Shemesh, AJ;Xiang, J;Thierry-Mieg, J;Thierry-Mieg, D;Iftner, A;Bezdan, D;Sanchez, E;Campion, TR;Sipley, J;Cong, L;Craney, A;Velu, P;Melnick, AM;Shapira, S;Hajirasouliha, I;Borczuk, A;Iftner, T;Salvatore, M;Loda, M;Westblade, LF;Cushing, M;Wu, S;Levy, S;Chiu, C;Schwartz, RE;Tatonetti, N;Rennert, H;Imielinski, M;Mason, CE;Shotgun transcriptome, spatial omics, and isothermal profiling of SARS-CoV-2 infection reveals unique host responses, viral diversification, and drug interactionsNature communications33712587Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.++Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.++Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, UFull Paper
2021Li, J;Conrad, C;Mills, TW;Berg, NK;Kim, B;Ruan, W;Lee, JW;Zhang, X;Yuan, X;Eltzschig, HK;PMN-derived netrin-1 attenuates cardiac ischemia-reperfusion injury via myeloid ADORA2B signalingThe Journal of experimental medicine33891683Materials and methods. MI patient and healthy donor samples. Serum/plasma samples of patients with MI or healthy donors were purchased from Discovery Life Sciences. Corresponding cTnI levels of patients with MI were documented in the patient sample dFull Paper
2021Dang, K;Castello, G;Clarke, SC;Li, Y;Balasubramani, A;Boudreau, A;Davison, L;Harris, KE;Pham, D;Sankaran, P;Ugamraj, HS;Deng, R;Kwek, S;Starzinski, A;Iyer, S;van Schooten, W;Schellenberger, U;Sun, W;Trinklein, ND;Buelow, R;Buelow, B;Fong, L;Dalvi, P;Attenuating CD3 affinity in a PSMAxCD3 bispecific antibody enables killing of prostate tumor cells with reduced cytokine releaseJournal for immunotherapy of cancer34088740Tumor cells were either isolated from fresh prostate tumor tissue procured from LF's lab at UCSF (San Francisco, California, USA) or received as frozen dissociated aliquots along with donor matched PBMCs from Discovery Life Sciences (Newtown, PennsylFull Paper
2021Nghiem, P;Bhatia, S;Lipson, EJ;Sharfman, WH;Kudchadkar, RR;Brohl, AS;Friedlander, PA;Daud, A;Kluger, HM;Reddy, SA;Boulmay, BC;Riker, A;Burgess, MA;Hanks, BA;Olencki, T;Kendra, K;Church, C;Akaike, T;Ramchurren, N;Shinohara, MM;Salim, B;Taube, JM;Jensen, E;Kalabis, M;Fling, SP;Homet Moreno, B;Sharon, E;Cheever, MA;Topalian, SL;Three-year survival, correlates and salvage therapies in patients receiving first-line pembrolizumab for advanced Merkel cell carcinomaJournal for immunotherapy of cancer33879601small T-antigen-specific serum antibodies21 or manifested large T-antigen expression in tumor biopsies via immunohistochemistry.22 PD-L1 staining (anti-PD-L1 clone 22C3, Merck & Co, Kenilworth, New Jersey, USA) was performed at QualTek MolecularFull Paper
2021Banchereau, R;Chitre, AS;Scherl, A;Wu, TD;Patil, NS;de Almeida, P;Kadel Iii, EE;Madireddi, S;Au-Yeung, A;Takahashi, C;Chen, YJ;Modrusan, Z;McBride, J;Nersesian, R;El-Gabry, EA;Robida, MD;Hung, JC;Kowanetz, M;Zou, W;McCleland, M;Caplazi, P;Eshgi, ST;Koeppen, H;Hegde, PS;Mellman, I;Mathews, WR;Powles, T;Mariathasan, S;Grogan, J;O'Gorman, WE;Intratumoral CD103+ CD8+ T cells predict response to PD-L1 blockadeJournal for immunotherapy of cancer33827905containing regimen. Fresh tumor samples and matched adjacent non-cancerous tissues were procured from a commercial vendor (Discovery Life Sciences) as part of adult patients undergoing surgical resection. Online supplemental ;h locally advancedFull Paper
2021Badve, SS;Penault-Llorca, F;Reis-Filho, JS;Deurloo, R;Siziopikou, KP;D'Arrigo, C;Viale, G;Determining PD-L1 Status in Patients with Triple-Negative Breast Cancer: Lessons Learned from IMpassion130Journal of the National Cancer Institute34286340CD has received consulting fees from Roche TD, Targos GmbH, Pfizer, Roche Pharma, LDPath Ltd, About Health Ltd, Uralensis INOV8 Ltd, and various NHS hospitals and has received nonfinan- cial support from UKNEQAS ICC&ISH and CADQAS cicFull Paper
2021Saura, C;Matito, J;Oliveira, M;Wildiers, H;Brufksy, AM;Waters, SH;Hurvitz, SA;Moy, B;Kim, SB;Gradishar, WJ;Queiroz, GS;Cronemberger, E;Wallweber, GJ;Bebchuk, J;Keyvanjah, K;Lalani, AS;Bryce, R;Vivancos, A;Eli, LD;Delaloge, S;Biomarker Analysis of the Phase III NALA Study of Neratinib + Capecitabine versus Lapatinib + Capecitabine in Patients with Previously Treated Metastatic Breast CancerClinical cancer research : an official journal of the American Association for Cancer Research34380637Yan (Puma Biotechnology). We thank Jeff Sperinde and Weidong Huang (Monogram Biosciences) for additional support for HERmark and p95 assay design and execution. We thank Sandra Bernard (Targos GMBH) for oversight of clinical sample management. WeFull Paper
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2021Larson, MH;Pan, W;Kim, HJ;Mauntz, RE;Stuart, SM;Pimentel, M;Zhou, Y;Knudsgaard, P;Demas, V;Aravanis, AM;Jamshidi, A;A comprehensive characterization of the cell-free transcriptome reveals tissue- and subtype-specific biomarkers for cancer detectionNature communications33883548We set out to validate the 23 cell-free mRNA biomarkers identified in our CCGA cohort in an orthogonal set of breast (n = 38) and lung (n = 18) cancer plasma samples obtained from a commercial vendor (Discovery Life Sciences) ;t to validate theFull Paper
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2021Egelston, C;Guo, W;Yost, S;Lee, JS;Rose, D;Avalos, C;Ye, J;Frankel, P;Schmolze, D;Waisman, J;Lee, P;Yuan, Y;Pre-existing effector T-cell levels and augmented myeloid cell composition denote response to CDK4/6 inhibitor palbociclib and pembrolizumab in hormone receptor-positive metastatic breast cancerJournal for immunotherapy of cancer33757987PD-L1 stain was performed using 22C3 antibody by Qualtek Molecular LaboratoryFull Paper
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2021Quaas, A;Pamuk, A;Klein, S;Quantius, J;Rehkaemper, J;Barutcu, AG;Rueschoff, J;Zander, T;Gebauer, F;Hillmer, A;Buettner, R;Schroeder, W;Bruns, CJ;Löser, H;Schoemig-Markiefka, B;Alakus, H;Sex-specific prognostic effect of CD66b-positive tumor-infiltrating neutrophils (TANs) in gastric and esophageal adenocarcinomaGastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association34009535Thomas Zander. Institute of Pathology, Nordhessen and Targos Molecular Pathology GmbH, Kassel, Germany. Josef Rueschoff. Authors: Alexander Quaas View author publications. You can also search for this author in PubMed Google ScholarFull Paper
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2021Narla, RK;Modi, H;Bauer, D;Abbasian, M;Leisten, J;Piccotti, JR;Kopytek, S;Eckelman, BP;Deveraux, Q;Timmer, J;Zhu, D;Wong, L;Escoubet, L;Raymon, HK;Hariharan, K;Modulation of CD47-SIRPα innate immune checkpoint axis with Fc-function detuned anti-CD47 therapeutic antibodyCancer immunology, immunotherapy : CII34247273lenalidomide [30]. Cell samples from AML patients were obtained from Conversant Bio (Huntsville) and are described in Supplemental Table 1. CC-90002 binding on cells and blockade of SIRPα to CD47 positive cells. Evaluation ofFull Paper
2021Vos, H;Lambein, K;Richard, F;Mariën, B;Nevelsteen, I;Punie, K;Wildiers, H;Berben, L;Laenen, A;Floris, G;Desmedt, C;Smeets, A;Comparison of the tumor immune microenvironment of primary hormone receptor-negative HER2-positive and triple negative breast cancerNPJ breast cancer34556657PD-L1 (22C3 antibody, 2 μg/mL, Dako-Agilent) immunohistochemistry was performed by Qualtek Molecular Laboratories (Newtown PA, USA) as per agreement with Merck. QualTek provided a modified proportion score (MPSFull Paper
2021Pierson, SK;Shenoy, S;Oromendia, AB;Gorzewski, AM;Langan Pai, RA;Nabel, CS;Ruth, JR;Parente, SAT;Arenas, DJ;Guilfoyle, M;Reddy, M;Weinblatt, M;Shadick, N;Bower, M;Pria, AD;Masaki, Y;Katz, L;Mezey, J;Beineke, P;Lee, D;Tendler, C;Kambayashi, T;Fosså, A;van Rhee, F;Fajgenbaum, DC;Discovery and validation of a novel subgroup and therapeutic target in idiopathic multicentric Castleman diseaseBlood advances34438448this study. We wish to thank Daniel Martinez and the other members of the CHOP Pathology Core as well as Qualtek for their immunohistochemical analyses. We wish to thank Kevin Silk, Giovanni Insana, and James A. Lebovitz for their contributions to thFull Paper
2021Wang, A;Song, Z;Zheng, G;Nicolazzi, C;Fromm, JR;Shehu, E;Srinivasan, S;Chen, X;Zhu, C;Blondel, MC;Adrian, FJ;Evaluation of Preclinical Activity of Isatuximab in Patients with Acute Lymphoblastic LeukemiaMolecular cancer therapeutics34376579centrifugation in line with the manufacturer's instructions. Patient ALL PBMCs were obtained from Conversant Biologics, Inc. Antibodies and reagents Isatuximab was generated in-house. Human immunoglobulin (Ig) G1, IgG4, ATRAFull Paper
2021Reardon, DA;Kim, TM;Frenel, JS;Simonelli, M;Lopez, J;Subramaniam, DS;Siu, LL;Wang, H;Krishnan, S;Stein, K;Massard, C;Treatment with pembrolizumab in programmed death ligand 1-positive recurrent glioblastoma: Results from the multicohort phase 1 KEYNOTE-028 trialCancer33496357QualTek Molecular Laboratories evaluated archived or newly obtained formalin-fixed, paraffin-embedded tumor samples using the 22C3 PD-L1 antibody clone (Merck & Co., Inc., Kenilworth, NJ, USA) and a laboratory- developed prototype immunohistochemicalFull Paper
2021Preillon, J;Cuende, J;Rabolli, V;Garnero, L;Mercier, M;Wald, N;Pappalardo, A;Denies, S;Jamart, D;Michaux, AC;Pirson, R;Pitard, V;Bagot, M;Prasad, S;Houthuys, E;Brouwer, M;Marillier, R;Lambolez, F;Marchante, JR;Nyawouame, F;Carter, MJ;Baron-Bodo, V;Marie-Cardine, A;Cragg, M;Déchanet-Merville, J;Driessens, G;Hoofd, C;Restoration of T-cell Effector Function, Depletion of Tregs, and Direct Killing of Tumor Cells: The Multiple Mechanisms of Action of a-TIGIT Antagonist AntibodiesMolecular cancer therapeutics33277440Cryopreserved isolated PBMCs from healthy volunteers and patients with cancer were provided by ImmunXperts and ConversantBio, respectively. PBMCs were thawed, resuspended in RPMI medium supplemented with 10% FBS + 50 U/mL penicillin/streptomycin (WesFull Paper
2021MacFarlane, AW;Yeung, HM;Alpaugh, RK;Dulaimi, E;Engstrom, PF;Dasari, A;Campbell, KS;Vijayvergia, N;Impacts of pembrolizumab therapy on immune phenotype in patients with high-grade neuroendocrine neoplasmsCancer immunology, immunotherapy : CII33398390PD-L1 immunohistochemistry (IHC) assay was performed at Qualtek Research Laboratories on formalin-fixed, paraffin-embedded tissue sections using anti-PD-L1 monoclonal antibody clone 22C3 (Merck Research Laboratories)Full Paper
2021Holder, C;Adams, A;Allison, C;Cote, O;Lippens, R;Blount, BC;Wang, L;A Novel UHPLC-MS/MS Method for Measuring 8-iso-Prostaglandin F2α in Bronchoalveolar Lavage FluidFrontiers in chemistry34476231Non-EVALI BAL fluids used in this study were acquired from Discovery Life Sciences (Huntsville, AL, United States)Full Paper
2021Rodrigues, D;de Souza, T;Coyle, L;Di Piazza, M;Herpers, B;Ferreira, S;Zhang, M;Vappiani, J;Sévin, DC;Gabor, A;Lynch, A;Chung, SW;Saez-Rodriguez, J;Jennen, DGJ;Kleinjans, JCS;de Kok, TM;New insights into the mechanisms underlying 5-fluorouracil-induced intestinal toxicity based on transcriptomic and metabolomic responses in human intestinal organoidsArchives of toxicology341514005-Fluorouracil (5-FU) is a widely used chemotherapeutical that induces acute toxicity in the small and large intestine of patients. Symptoms can be severe. ;- 5-Fluorouracil (5-FU) is a widely used chemotherapeutical that induces acute toxicity inFull Paper
2021Comunale, BA;Engineer, L;Jiang, Y;Andrews, JC;Liu, Q;Ji, L;Yurkovich, JT;Comunale, RA;Xie, Q;Poliovirus Vaccination Induces a Humoral Immune Response That Cross Reacts With SARS-CoV-2Frontiers in medicine34414206ccination protocols, set by the Centers for Disease Control and Prevention (CDC). IRB approval for the collection of adult sera was obtained from BioMed IRB, and pediatric samples were purchased from Discovery Life Sciences (Huntsville, AL) in accordFull Paper
2021Haynes, WA;Kamath, K;Waitz, R;Daugherty, PS;Shon, JC;Protein-Based Immunome Wide Association Studies (PIWAS) for the Discovery of Significant Disease-Associated AntigensFrontiers in immunology33986742years, with a range of 22-72. Anti-Smith Cohort Samples from 34 subjects that tested positive for Anti-SM RNP (4) or Anti-Smith (30) antibodies by predicate ANA multiplex testing were obtained from Discovery Life Sciences. Subjects ranged in age froFull Paper
2021Kerr, KM;Bibeau, F;Thunnissen, E;Botling, J;Ryška, A;Wolf, J;Öhrling, K;Burdon, P;Malapelle, U;Büttner, R;The evolving landscape of biomarker testing for non-small cell lung cancer in EuropeLung cancer (Amsterdam, Netherlands)33690091He is a co-owner and CSO of Targos Molecular Pathology, Inc., GermanyFull Paper
2021Gentzel, RC;Toolan, D;Jinn, S;Schachter, JB;Ma, L;Kahle, PJ;Smith, SM;Marcus, JN;Intracranial administration of alpha-synuclein fibrils in A30P-synuclein transgenic mice causes robust synucleinopathy and microglial inductionNeurobiology of aging34225000comparison. Neuropathologically confirmed human post-mortem PD brain tissue, acquired from Folio/Discovery Life Sciences (California, USA), or A30P HET brain tissues were serially extracted to obtain a sarkosyl insoluble fractionFull Paper
2021Malik-Chaudhry, HK;Prabhakar, K;Ugamraj, HS;Boudreau, AA;Buelow, B;Dang, K;Davison, LM;Harris, KE;Jorgensen, B;Ogana, H;Pham, D;Schellenberger, U;Van Schooten, W;Buelow, R;Iyer, S;Trinklein, ND;Rangaswamy, US;TNB-486 induces potent tumor cell cytotoxicity coupled with low cytokine release in preclinical models of B-NHLmAbs33818299ground value. The percent of specific lysis was calculated as described above. Ex vivo cytotoxicity assay The ability of TNB-486 to kill CD19+ tumor cells from CLL or DLBCL patient-derived samples (Discovery Life Sciences and IQ Biosciences) was detFull Paper
2021Yamada, M;Eguchi, A;Okuno, K;Sakaguchi, K;Yamaguchi, T;Development of a highly sensitive chemiluminescent enzyme immunoassay for fragmented cytokeratin 18 using new antibodiesScientific reports34521905All methods were carried out in accordance with relevant guidelines and regulations. Human samples. Serum from healthy individuals and NASH patients was purchased from Discovery Life Sciences (CA, USA) with approval and informed consent;defined belowFull Paper
2021Awad, H;Tili, E;Nuovo, G;Kelani, H;Ramadan, ME;Williams, J;Binzel, K;Rajan, J;Mast, D;Efanov, AA;Rasul, KB;Moore, S;Basso, M;Mikhail, A;Eltobgy, M;Malbrue, RA;Bourekas, E;Oglesbee, M;Bergdall, V;Knopp, M;Michaille, JJ;El-Sayed, H;Endovascular repair and open repair surgery of thoraco-abdominal aortic aneurysms cause drastically different types of spinal cord injuryScientific reports33837260The remainder of the tissue was stored at − 80 °C for metabolome analyses. All sections were subsequently analyzed blindly. Immunohistochemistry (IHC). All sections were dealt with blindly using an automated Leica Bond Max platform at Phylogeny Inc.Full Paper
2021Mezache, L;Nuovo, G;Veeraraghavan, R;A Multipronged Microscopy Approach Identifies Common Anti-Arrhythmic Strategy for Atrial Fibrillation and Myocardial InfarctionMicroscopy and Microanalysis1 The Ohio State University, United States, 2 Discovery Life Sciences, United States Vascular leak and inflammation are associated with arrhythmic heart diseases such as atrial fibrillation (AF) and myocardial infarction (MI)Full Paper
2021Ermann, J;Matmusaev, M;Haley, E;Braun, C;Jost, F;Mayer-Wrangowski, S;Hsiao, P;Ting, N;Li, L;Terenzio, D;Chime, J;Lukas, S;Patnaude, L;Panzenbeck, M;Csordas, D;Zheng, J;Mierz, D;Simpson, T;King, FJ;Klimowicz, A;Mbow, ML;Fine, JS;Miller, C;Fogal, S;Byrne, FR;The potent and selective RIPK2 inhibitor BI 706039 improves intestinal inflammation in the TRUC mouse model of Inflammatory Bowel DiseaseAmerican journal of physiology. Gastrointestinal and liver physiology34494462Human IBD Intestinal biopsy Immuno-histo-chemical analysis 231 Tissue blocks from surgical resections for ulcerative colitis (n=6) and Crohn's Disease (n=5) were 232 acquired from Discovery Life Sciences. 4 µm sections were fluorescently stained usinFull Paper
2021Reisenauer, KN;Tao, Y;Das, P;Song, S;Svatek, H;Patel, SD;Mikhail, S;Ingros, A;Sheesley, P;Masi, M;Boari, A;Evidente, A;Kornienko, A;Romo, D;Taube, J;Epithelial-mesenchymal transition sensitizes breast cancer cells to cell death via the fungus-derived sesterterpenoid ophiobolin AScientific reports34017048ke to acknowledge Dr. Michelle Nemec, Director of the Molecular Bioscience Center (Baylor University) for assistance with flow cytometry and Dr. Maria Boccia (Baylor University) and Dr. Gerard Nuovo (Discovery Life Sciences, Powell, OH, USA) for assiFull Paper
2021Caldwell, C;Rottman, JB;Paces, W;Bueche, E;Reitsma, S;Gibb, J;Adisetiyo, V;Haas, MS;Heath, H;Newman, W;Baum, J;Gianani, R;Kagey, MH;Validation of a DKK1 RNAscope chromogenic in situ hybridization assay for gastric and gastroesophageal junction adenocarcinoma tumorsScientific reports33972574chromogen kit (Biocare Medical). RNAscope. G/GEJ FFPE tumor resections were commercially acquired from Avaden Biosciences, Discovery Life Sciences or BioOptions following IRB compliance. RNAscope was performed ;emperature for 30 min, washed, andFull Paper
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2021Lopez-Anido, RN;Harrington, AM;Hamlin, HJ;Coping with stress in a warming Gulf: the postlarval American lobster's cellular stress response under future warming scenariosCell stress & chaperones34115338The RNA samples from five replicate animals reared at each temperature were shipped to HudsonAlpha Discovery (formerly the Genomic Services Lab) at the HudsonAlpha Institute for Biotechnology (Huntsville, AL, USA) for library preparation and sequenciFull Paper
2021Magro, C;Crowson, A;Franks, L;Schaffer, P;Whelan, P;Nuovo, G;The histologic and molecular correlates of COVID-19 vaccine-induced changes in the skinClinics in Dermatologyonald O. Perelman Department of Dermatology, New York University Grossman School of Medicine, New York, New York eDepartment of Pediatrics, Geffen School of Medicine at UCLA, Los Angeles, California fDiscovery Life Sciences, Powell, Ohio gThe Ohio StFull Paper
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2021Cirillo, PDR;Margiotti, K;Fabiani, M;Barros-Filho, MC;Sparacino, D;Cima, A;Longo, SA;Cupellaro, M;Mesoraca, A;Giorlandino, C;Multi-analytical test based on serum miRNAs and proteins quantification for ovarian cancer early detectionPloS one34352040All serum samples from ovarian cancer patients were obtained through MTA (Material Transfer Agreement) signed consent form from the following centers: Discovery Life Sciences (Huntsville, USA), BioIVT (London, UK), Victorian Cancer Bank (Melbourne, AFull Paper
2021Walsh, CS;Kamrava, M;Rogatko, A;Kim, S;Li, A;Cass, I;Karlan, B;Rimel, BJ;Phase II trial of cisplatin, gemcitabine and pembrolizumab for platinum-resistant ovarian cancerPloS one34081738that had membrane staining at low (1+) intensities or greater. An H-score was calculated ranging from 0–300, reflecting the percentage of cells staining at each of the following intensities: negative (1), low (1+), moderate (2+), high (3+). IHC assayFull Paper
2021Bernstein, DE;Trinh, HN;Schiff, ER;Smith, CI;Mospan, AR;Zink, RC;Fried, MW;Lok, AS;Safety and Effectiveness of Tenofovir Alafenamide in Usual Clinical Practice Confirms Results of Clinical Trials: TARGET-HBVDigestive diseases and sciences34059991ERS: Grant support: Eiger, Galmed, Zydus, Celgene, Beckman, Biokit, Bristol Myers Squibb, Conatus, Celgene, Discovery Life Sciences, Genfit, Gilead, Intercept, Novartis, Novo Nordisk, Orasure Technologies, Ortho Diagnostics, Pfizer, Prometheus Lab, RFull Paper
2021Varlet, P;Bouffet, E;Casanova, M;Giangaspero, F;Antonelli, M;Hargrave, D;Ladenstein, R;Pearson, A;Hawkins, C;König, FB;Rüschoff, J;Schmauch, C;Bühnemann, C;Garin-Chesa, P;Schweifer, N;Uttenreuther-Fischer, M;Gibson, N;Ittrich, C;Krämer, N;Solca, F;Stolze, B;Geoerger, B;Comprehensive analysis of the ErbB receptor family in pediatric nervous system tumors and rhabdomyosarcomaPediatric blood & cancer34546642Institute, Medical University, Vienna, Austria 8 Paediatric Drug Development, Children and Young People's Unit, Royal Marsden Hospital, London, UK 9 Division of Clinical Studies, Institute of Cancer Research, London, UK 10 Targos Molecular PathologyFull Paper
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2021Gaidt, MM;Morrow, A;Fairgrieve, MR;Karr, JP;Yosef, N;Vance, RE;Self-guarding of MORC3 enables virulence factor-triggered immunityNature34759314(PBMCs) from de-identified donors were obtained from AllCells (Alameda) under donor-informed consent and Alpha IRB approval obtained by AllCells (7000-SOP-045) for the study ' SaveFull Paper
2021Mimitou, EP;Lareau, CA;Chen, KY;Zorzetto-Fernandes, AL;Hao, Y;Takeshima, Y;Luo, W;Huang, TS;Yeung, BZ;Papalexi, E;Thakore, PI;Kibayashi, T;Wing, JB;Hata, M;Satija, R;Nazor, KL;Sakaguchi, S;Ludwig, LS;Sankaran, VG;Regev, A;Smibert, P;Scalable, multimodal profiling of chromatin accessibility, gene expression and protein levels in single cellsNature biotechnology34083792Cryopreserved healthy donor peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BM) were obtained from AllCells (USA) or Cellular Technology Limited (CTL) and processed immediately after thawing. NIH-3T3 and HEK293FT cells were maintainFull Paper
2021Lareau, CA;Ludwig, LS;Muus, C;Gohil, SH;Zhao, T;Chiang, Z;Pelka, K;Verboon, JM;Luo, W;Christian, E;Rosebrock, D;Getz, G;Boland, GM;Chen, F;Buenrostro, JD;Hacohen, N;Wu, CJ;Aryee, MJ;Regev, A;Sankaran, VG;Massively parallel single-cell mitochondrial DNA genotyping and chromatin profilingNature biotechnology32788668Cryopreserved PBMCs from patients with CLL consented on institutional review board approved protocols were obtained from AllCells (Patient 1) or from Adrian Wiestner at the SaveFull Paper
2021Hao, Y;Hao, S;Andersen-Nissen, E;Mauck, WM;Zheng, S;Butler, A;Lee, MJ;Wilk, AJ;Darby, C;Zager, M;Hoffman, P;Stoeckius, M;Papalexi, E;Mimitou, EP;Jain, J;Srivastava, A;Stuart, T;Fleming, LM;Yeung, B;Rogers, AJ;McElrath, JM;Blish, CA;Gottardo, R;Smibert, P;Satija, R;Integrated analysis of multimodal single-cell dataCell34062119stom made clone UCHL1 ------------------------- Biological samples ------------------------- Human PBMC Cape Town HVTN Immunology Lab, South Africa https://www.chil.org.za/ Human PBMC AllCells Lot 3032552 -------------------------Full Paper
2021Okuchi, Y;Reeves, J;Ng, SS;Doro, DH;Junyent, S;Liu, KJ;El Haj, AJ;Habib, SJ;Wnt-modified materials mediate asymmetric stem cell division to direct human osteogenic tissue formation for bone repairNature materials32958876hSSCs were isolated from a commercially available bone marrow aspirate (AllCells). The bone marrow aspirate was plated onto culture flasks precoated with 10 ng ml−1 fibronectin (Sigma-Aldrich, 356000) and the cells were allowed to attach. Surface marFull Paper
2021Wu, SZ;Al-Eryani, G;Roden, DL;Junankar, S;Harvey, K;Andersson, A;Thennavan, A;Wang, C;Torpy, JR;Bartonicek, N;Wang, T;Larsson, L;Kaczorowski, D;Weisenfeld, NI;Uytingco, CR;Chew, JG;Bent, ZW;Chan, CL;Gnanasambandapillai, V;Dutertre, CA;Gluch, L;Hui, MN;Beith, J;Parker, A;Robbins, E;Segara, D;Cooper, C;Mak, C;Chan, B;Warrier, S;Ginhoux, F;Millar, E;Powell, JE;Williams, SR;Liu, XS;O'Toole, S;Lim, E;Lundeberg, J;Perou, CM;Swarbrick, A;A single-cell and spatially resolved atlas of human breast cancersNature genetics34493872Percentage of neoplastic cells in each tumor that are classified as each of the SCSubtypes. Tumor samples are grouped according to their Allcells-Pseudobulk classifications. NL, normal-like. c, Representative images of CK5 (top) and ER (bottom) IHC fFull Paper
2021Ge, Y;Tian, T;Huang, S;Wan, F;Li, J;Li, S;Wang, X;Yang, H;Hong, L;Wu, N;Yuan, E;Luo, Y;Cheng, L;Hu, C;Lei, Y;Shu, H;Feng, X;Jiang, Z;Wu, Y;Chi, Y;Guo, X;Cui, L;Xiao, L;Li, Z;Yang, C;Miao, Z;Chen, L;Li, H;Zeng, H;Zhao, D;Zhu, F;Shen, X;Zeng, J;An integrative drug repositioning framework discovered a potential therapeutic agent targeting COVID-19Signal transduction and targeted therapy33895786e performed at a flow rate of 30 μL/min. Data processing and analyses were performed using BIAevaluation 1.1 software. LPS-induced cytokine production in PBMCs Peripheral blood mononuclear cells (Allcells) were cultured at 37 °C at concentration 5%Full Paper
2021Miller, IC;Zamat, A;Sun, LK;Phuengkham, H;Harris, AM;Gamboa, L;Yang, J;Murad, JP;Priceman, SJ;Kwong, GA;Enhanced intratumoural activity of CAR T cells engineered to produce immunomodulators under photothermal controlNature biomedical engineering34385695Primary human CD3+ cells were obtained from an anonymous donor blood after apheresis (AllCells) and were cryopreserved in 90% FBS and 10% dimethylsulfoxide until subsequent SaveFull Paper
2021Frede, J;Anand, P;Sotudeh, N;Pinto, RA;Nair, MS;Stuart, H;Yee, AJ;Vijaykumar, T;Waldschmidt, JM;Potdar, S;Kloeber, JA;Kokkalis, A;Dimitrova, V;Mann, M;Laubach, JP;Richardson, PG;Anderson, KC;Raje, NS;Knoechel, B;Lohr, JG;Dynamic transcriptional reprogramming leads to immunotherapeutic vulnerabilities in myelomaNature cell biology34675390registration was 68.5 years (range 45-80). Myeloma cells were enriched using the EasySep Human CD138 positive selection kit (STEMCELL Technologies). Bone marrow from normal donors was obtained from AllCells. Single cell sorting Bone marrow cells werFull Paper
2021Duault, C;Kumar, A;Taghi Khani, A;Lee, SJ;Yang, L;Activated natural killer cells predict poor clinical prognosis in high-risk B-and T-cell acute lymphoblastic leukemiablood36107453processed from patients with B/T-ALL and healthy donors according to Institutional Review Board policies. Healthy BMMCs were purchased from AllCells (Alameda, CA). Healthy PBMCs were isolated from buffy coats procured from Stanford Blood Center, CityFull Paper
2021Duault, C;Kumar, A;Taghi Khani, A;Lee, SJ;Yang, L;Huang, M;Hurtz, C;Manning, B;Ghoda, L;McDonald, T;Lacayo, NJ;Sakamoto, KM;Carroll, M;Tasian, SK;Marcucci, G;Yu, J;Caligiuri, MA;Maecker, HT;Swaminathan, S;Activated natural killer cells predict poor clinical prognosis in high-risk B- and T-cell acute lymphoblastic leukemiaBlood34077953ripheral blood mononuclear cells (PBMCs) were collected and processed from patients with B/T-ALL and healthy donors according to Institutional Review Board policies. Healthy BMMCs were purchased from AllCells (Alameda, CA). Healthy PBMCs were isolateFull Paper
2021Alavi, A;Krishnamurti, L;Abedi, M;Galeon, I;Reiner, D;Smith, S;Wang, L;Ramezi, A;Rendo, P;Walters, M;Preliminary Safety and Efficacy Results from Precizn-1: An Ongoing Phase 1/2 Study on Zinc Finger Nuclease-Modified Autologous CD34+ HSPCs for Sickle Cell Disease (SCD)BloodIntroduction Sickle cell disease (SCD) is caused by pathologic variants in both alleles of the β-globin gene, affecting ~100,000 patients in the US (Strouse. Handb Clin Neurol 2016;138:311-24). Elevated fetal hemoglobin (HbF) levels amFull Paper
2021Kwiatkowski, J;Locatelli, F;Walters, M;Porter, J;Hongeng, S;Yannaki, E;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Guo, R;Colvin, R;Gruppioni, K;Thompson, A;Improvement in Health-Related Quality of Life Following Treatment with Betibeglogene Autotemcel in Patients with Transfusion-Dependent β-Thalassemia Enrolled in Phase 3 StudiesBloodIntroduction: Patients with transfusion-dependent β-thalassemia (TDT) have increased morbidity and mortality and reduced health-related quality of life (HRQoL) compared with the general population owing to the mental, physical, and time demands of chFull Paper
2021Hall, A;Winestone, L;Sullivan, E;Wu, Q;Lamble, A;Walters, M;Aguayo-Hiraldo, P;Baez Conde, L;Coker, T;Dave, H;Dornsife, D;Keating, A;Merino, D;Ramsey, B;Park, J;Agrawal, A;Access to CAR-T Cell Therapy in Underrepresented Populations: A Multicenter Cohort Study of Pediatric and Young Adult ALL PatientsBloodIntroduction: Social determinants of health such as race, ethnicity, and socioeconomic status (SES) are associated with inferior outcomes in patients with B-cell acute lymphoblastic leukemia (B-ALL). Latinx patients have worse event-free survival andFull Paper
2021Thompson, A;Locatelli, F;Yannaki, E;Walters, M;Porter, J;Hongeng, S;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Cavazzana, M;Rasko, J;Du, L;Colvin, R;Kwiatkowski, J;Restoring Iron Homeostasis in Pts Who Achieved Transfusion Independence after Treatment with Betibeglogene Autotemcel Gene Therapy: Results from up to 7 Years of Follow-upBloodIntroduction: Transfusion-dependent β-thalassemia (TDT) is a severe genetic disease requiring lifelong, regular packed red blood cell (pRBC) transfusions and iron chelation treatment. Betibeglogene autotemcel (beti-cel) is a one-time ex vivo gene theFull Paper
2021Walters, M;Smith, A;Schiller, G;Esrick, E;Williams, D;Gogoleva, T;Rouy, D;Cockroft, B;Vercellotti, G;Updated Results of a Phase 1/2 Clinical Study of Zinc Finger Nuclease-Mediated Editing of BCL11A in Autologous Hematopoietic Stem Cells for Transfusion-Dependent Beta ThalassemiaBloodIntroduction: Persistently elevated fetal hemoglobin (HbF) can ameliorate transfusion-dependent beta thalassemia (TDT) (Musallam et al ., 2012). BCL11A is a repressor of γ-globin expression and HbF production in adult erythrocytes. Down-regulation ofFull Paper
2021Tisdale, J;Thompson, A;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Schmidt, M;Pierciey, F;Whitney, D;Rogers, C;Nnamani, M;Foos, M;Miller, A;Zhang, X;Lynch, J;Walters, M;Kanter, J;Bonner, M;Polyclonality Strongly Correlates with Biological Outcomes and Is Significantly Increased Following Improvements to the Phase 1/2 HGB-206 Protocol and Manufacturing of LentiGlobin for Sickle Cell Disease (SCD; bb1111) Gene Therapy (GT)BloodBackground: The ongoing Phase 1/2 HGB-206 study (NCT02140554) of LentiGlobin for SCD (bb1111) GT uses a modified human β-globin gene that expresses an anti-sickling hemoglobin (HbA T87Q). The relationships between biological outcomes, clinical outcomFull Paper
2021Walters, M;Tisdale, J;Mapara, M;Krishnamurti, L;Kwiatkowski, J;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Jaroscak, J;Garbinsky, D;Chirila, C;Gallagher, M;Zhang, X;Ho, P;Thompson, A;Kanter, J;Sustained Improvements in Patient-Reported Quality of Life up to 24 Months Post-Treatment with LentiGlobin for Sickle Cell Disease (bb1111) Gene TherapyBloodBackground: Sickle cell disease (SCD) is characterized by painful vaso-occlusive events (VOEs), progressive vasculopathy, hemolytic anemia, and organ damage resulting in frequent hospitalizations and decreased quality of life (QoL). The ongoing phaseFull Paper
2021Kruta, M;Sunshine, MJ;Chua, BA;Fu, Y;Chawla, A;Dillingham, CH;Hidalgo San Jose, L;De Jong, B;Zhou, FJ;Signer, RAJ;Hsf1 promotes hematopoietic stem cell fitness and proteostasis in response to ex vivo culture stress and agingCell stem cell34388375Human cord blood derived CD34 + HSPCs were purchased from AllCells. Cell number and viability were assessed with a hemocytometer based on trypan blue exclusion. SaveFull Paper
2021Turchiano, G;Andrieux, G;Klermund, J;Blattner, G;Pennucci, V;El Gaz, M;Monaco, G;Poddar, S;Mussolino, C;Cornu, TI;Boerries, M;Cathomen, T;Quantitative evaluation of chromosomal rearrangements in gene-edited human stem cells by CAST-SeqCell stem cell33626327Experimental models: cell lines human CD34+ HSPCs AllCells Cat.# CB008F human CD34+ HSPCs StemCell-Technologies Cat.# 70008.3 K562 N/A RRID:CVCL_0004; authenticated by STR profilingFull Paper
2021Mondala, PK;Vora, AA;Zhou, T;Lazzari, E;Ladel, L;Luo, X;Kim, Y;Costello, C;MacLeod, AR;Jamieson, CHM;Crews, LA;Selective antisense oligonucleotide inhibition of human IRF4 prevents malignant myeloma regeneration via cell cycle disruptionCell stem cell33476575In multiple myeloma, inflammatory and anti-viral pathways promote disease progression and cancer stem cell generation. Using diverse pre-clinical models, we investigated the role of SaveFull Paper
2021Su, M;Lin, Y;Cui, C;Tian, X;Lai, L;ERMAP is a B7 family-related molecule that negatively regulates T cell and macrophage responsesCellular & molecular immunology32620788T cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan-T labeling system were purchased from ALLCELLS, LLC (Alameda, CA). Murine CD3+ T cells were purified from C57BL/6 mice by an immunomagnetic system (MiltFull Paper
2021Seo, H;Masuda, H;Asagoshi, K;Uchiki, T;Kawata, S;Sasaki, G;Yabuki, T;Miyai, S;Takahashi, N;Hashimoto, SI;Sawada, A;Takaiwa, A;Koyama, C;Tamai, K;Kurosawa, K;Lin, KY;Ohta, K;Nakazaki, Y;Streamlined human antibody generation and optimization by exploiting designed immunoglobulin loci in a B cell lineCellular & molecular immunology32457406The germline-derived divergent hV H harboring CDR3 was prepared from normal human peripheral blood CD19 + B-cell cDNA (ALLCELLS) using degenerate primers that annealed to SaveFull Paper
2021Sadras, T;Martin, M;Kume, K;Robinson, ME;Saravanakumar, S;Lenz, G;Chen, Z;Song, JY;Siddiqi, T;Oksa, L;Knapp, AM;Cutler, J;Cosgun, KN;Klemm, L;Ecker, V;Winchester, J;Ghergus, D;Soulas-Sprauel, P;Kiefer, F;Heisterkamp, N;Pandey, A;Ngo, V;Wang, L;Jumaa, H;Buchner, M;Ruland, J;Chan, WC;Meffre, E;Martin, T;Müschen, M;Developmental partitioning of SYK and ZAP70 prevents autoimmunity and cancerMolecular cell33878293Cord blood derived human CD34 + cells (ALLCELLS) were thawed and were lentivirally transduced with pCL6-ZAP70-GFP or an empty vector control. To obtain optimal transduction, SaveFull Paper
2021Lattanzi, A;Camarena, J;Lahiri, P;Segal, H;Srifa, W;Vakulskas, CA;Frock, RL;Kenrick, J;Lee, C;Talbott, N;Skowronski, J;Cromer, MK;Charlesworth, CT;Bak, RO;Mantri, S;Bao, G;DiGiusto, D;Tisdale, J;Wright, JF;Bhatia, N;Roncarolo, MG;Dever, DP;Porteus, MH;Development of β-globin gene correction in human hematopoietic stem cells as a potential durable treatment for sickle cell diseaseScience translational medicine34135108Human CD34+ HSPCs were cultured in small-scale conditions as previously described (67, 20, 25, 68, 69). Frozen purified CD34+ HSPCs were sourced from plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA and STEMCELL TechnolFull Paper
2021Jain, MS;Polanski, K;Conde, CD;Chen, X;Park, J;Mamanova, L;Knights, A;Botting, RA;Stephenson, E;Haniffa, M;Lamacraft, A;Efremova, M;Teichmann, SA;MultiMAP: dimensionality reduction and integration of multimodal dataGenome biology34930412sed to identify genes whose expression changes significantly along the trajectory. Acquisition and processing of human PBMCs PBMCs from two donors were acquired from a LeukoLab (Clinical division of AllCells). Frozen PBMC samples were thawed quicklyFull Paper
2021Thongon, N;Ma, F;Santoni, A;Marchesini, M;Fiorini, E;Rose, A;Adema, V;Ganan-Gomez, I;Groarke, EM;Gutierrez-Rodrigues, F;Chen, S;Lockyer, P;Schneider, S;Bueso-Ramos, C;Montalban-Bravo, G;Class, CA;Soltysiak, KA;Pellegrini, M;Sahin, E;Bertuch, AA;DiNardo, CD;Garcia-Manero, G;Young, NS;Dwyer, K;Colla, S;Hematopoiesis under telomere attrition at the single-cell resolutionNature communications34824242BM samples from HDs were obtained from AllCells. Written informed consent was obtained from all donors. The clinical characteristics and the PB counts of the individuals with SaveFull Paper
2021Qiao, Y;Qiu, Y;Ding, J;Luo, N;Wang, H;Ling, X;Sun, J;Wu, Z;Wang, Y;Liu, Y;Guo, F;Sun, T;Shen, W;Zhang, M;Wu, D;Chen, B;Xu, W;Wang, X;Cancer immune therapy with PD-1-dependent CD137 co-stimulation provides localized tumour killing without systemic toxicityNature communications34737267Human PBMCs were purchased from Allcells and the informed consent was obtained from all donors. Human PBMCs were cultured in RPMI 1640 medium supplemented with 10% FBS SaveFull Paper
2021Duren, Z;Lu, WS;Arthur, JG;Shah, P;Xin, J;Meschi, F;Li, ML;Nemec, CM;Yin, Y;Wong, WH;Sc-compReg enables the comparison of gene regulatory networks between conditions using single-cell dataNature communications34362918itial analysis has considered the HiChIP data. We will extend our sc-compReg software to enable this type of analysis. Methods Single-cell ATAC Cryopreserved BMMCs from two donors were obtained from AllCells. One donor was a 93-year-old non-HispanicFull Paper
2021Amit, I;Iancu, O;Levy-Jurgenson, A;Kurgan, G;McNeill, MS;Rettig, GR;Allen, D;Breier, D;Ben Haim, N;Wang, Y;Anavy, L;Hendel, A;Yakhini, Z;CRISPECTOR provides accurate estimation of genome editing translocation and off-target activity from comparative NGS dataNature communications34031394ubation at room temperature before the electroporation. Genome editing in mobilized CD34+ HSPCs and in HEK293/HEK293-Cas9 cell lines Human CD34+ HSPCs, derived from mobilized peripheral blood (MPB) (AllCells, Alameda, CA), were thawed and cultured fFull Paper
2021Lal, A;Chiang, ZD;Yakovenko, N;Duarte, FM;Israeli, J;Buenrostro, JD;Deep learning-based enhancement of epigenomics data with AtacWorksNature communications336860690 or 1 representing whether that position was part of a CTCF binding motif on the reverse strand. Generation of dscATAC-seq data for FACS-isolated HSCs Cryopreserved human BMMCs were purchased from Allcells (catalog number BM, CR, MNC, 10 M). CellsFull Paper
2021Sharma, R;Dever, DP;Lee, CM;Azizi, A;Pan, Y;Camarena, J;Köhnke, T;Bao, G;Porteus, MH;Majeti, R;The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cellsNature communications33473139For in vivo studies presented, cord blood-derived CD34 + cells were purchased from AllCells or STEMCELL Technologies and were thawed according to the manufacturer's SaveFull Paper
2021Yang, KD;Belyaeva, A;Venkatachalapathy, S;Damodaran, K;Katcoff, A;Radhakrishnan, A;Shivashankar, GV;Uhler, C;Multi-domain translation between single-cell imaging and sequencing data using autoencodersNature communications33397893nly limited functional data is available by acquiring chromatin imaging data. Methods Cell culture and immunostaning CD4+/CD45RA+ naive helper T-cells from human peripheral blood were purchased from AllCells. These cells were revived and cultured inFull Paper
2021Gnanapragasam, M;Planutis, A;Glassberg, J;Bieker, J;Identification of a genomic DNA sequence that quantitatively modulates KLF1 expression in differentiating human hematopoietic cellsbioRxivCD34+ cells were purchased from AllCells or obtained from the Yale Cooperative Center of Excellence in Hematology. These were differentiated under three-phase protocols, initially based on (Antoniani et al., 2018; Yien and Bieker, 2012) but then lateFull Paper
2021Shy, B;Vykunta, V;Ha, A;Roth, T;Talbot, A;Nguyen, D;Chen, Y;Blaeschke, F;Vedova, S;Mamedov, M;Chung, J;Li, H;Wolf, J;Martin, T;Ye, L;Eyquem, J;Esensten, J;Marson, A;Hybrid ssDNA repair templates enable high yield genome engineering in primary cells for disease modeling and cell therapy manufacturingbioRxivPrimary adult blood cells were obtained from anonymous healthy human donors as a leukapheresis pack purchased from StemCell Technologies, Inc. or Allcells Inc, or as a Trima residual from Vitalant. If needed, peripheral blood mononuclear cells were iFull Paper
2021Amin, OE;Colbeck, EJ;Daffis, S;Khan, S;Ramakrishnan, D;Pattabiraman, D;Chu, R;Micolochick Steuer, H;Lehar, S;Peiser, L;Palazzo, A;Frey, C;Davies, J;Javanbakht, H;Rosenberg, WMC;Fletcher, SP;Maini, MK;Pallett, LJ;Therapeutic Potential of TLR8 Agonist GS-9688 (Selgantolimod) in Chronic Hepatitis B: Remodeling of Antiviral and Regulatory MediatorsHepatology (Baltimore, Md.)33368377In select experiments, whole blood was obtained from AllCells (Alameda, CA) and the MT Group (Van Nuys, CA). In this instance, consent was obtained from the donor or donor's legal SaveFull Paper
2021Shukla, S;Ying, W;Gray, F;Yao, Y;Simes, ML;Zhao, Q;Miao, H;Cho, HJ;González-Alonso, P;Winkler, A;Lund, G;Purohit, T;Kim, E;Zhang, X;Ray, JM;He, S;Nikolaidis, C;Ndoj, J;Wang, J;Jaremko, Ł;Jaremko, M;Ryan, RJH;Guzman, ML;Grembecka, J;Cierpicki, T;Small-molecule inhibitors targeting Polycomb repressive complex 1 RING domainNature chemical biology34155404CD34-positive bone marrow blood cells were obtained from Allcells (ABM021F) and stored in liquid nitrogen until use. For treatment experiments that lasted more than 4 d, media were SaveFull Paper
2021Jang, S;Song, J;Kim, N;Bak, J;Jung, K;Park, YW;Park, BC;Kim, HM;Development of an antibody-like T-cell engager based on VH-VL heterodimer formation and its application in cancer therapyBiomaterials33774526All healthy donor PBMCs and CD8 + T cells used in this study were purchased from AllCells (#PB004F and #PB009-3 F), Zenbio (#SER-PBMC-200-F), and Lonza (#3W-270). CD3 + T SaveFull Paper
2021Davis-Marcisak, EF;Fitzgerald, AA;Kessler, MD;Danilova, L;Jaffee, EM;Zaidi, N;Weiner, LM;Fertig, EJ;Transfer learning between preclinical models and human tumors identifies a conserved NK cell activation signature in anti-CTLA-4 responsive tumorsGenome medicine34376232Fresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selected from donor peripheral blood using CD56 positivity. Donor NK cell purity SaveFull Paper
2021Steinbuck, MP;Seenappa, LM;Jakubowski, A;McNeil, LK;Haqq, CM;DeMuth, PC;A lymph node-targeted Amphiphile vaccine induces potent cellular and humoral immunity to SARS-CoV-2Science advances33547083scent serum/plasma Convalescent serum samples (n = 7) and plasma samples (n = 15) from patients who had recovered from SARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and AllCells (Alameda, CA), respectively. All samplesFull Paper
2021Lin, Y;Cui, C;Su, M;Silbart, LK;Liu, H;Zhao, J;He, L;Huang, Y;Xu, D;Wei, X;Du, Q;Lai, L;Identification of TAPBPL as a novel negative regulator of T-cell functionEMBO molecular medicine33938620itro T‐cell assays Normal human peripheral blood CD3+ Pan T cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan‐T labeling system were purchased from ALLCELLS, LLC (Alameda, CA). Murine CD3+ T cells were puFull Paper
2021Kim, WT;Hennick, K;Johnson, J;Finnerty, B;Choo, S;Short, SB;Drubin, C;Forster, R;McMaster, ML;Hockemeyer, D;Cancer-associated POT1 mutations lead to telomere elongation without induction of a DNA damage responseThe EMBO journal33934394e aging or age‐associated diseases (Chakravarti et al, 2021). Materials and Methods Cell culture and cryopreservation Human bone marrow CD34+ hematopoietic stem/progenitor cells were obtained from AllCells and Fred Hutchinson Cancer Research Center.Full Paper
2021Deegen, P;Thomas, O;Nolan-Stevaux, O;Li, S;Wahl, J;Bogner, P;Aeffner, F;Friedrich, M;Liao, MZ;Matthes, K;Rau, D;Rattel, B;Raum, T;Kufer, P;Coxon, A;Bailis, JM;The PSMA-targeting Half-life Extended BiTE Therapy AMG 160 has Potent Antitumor Activity in Preclinical Models of Metastatic Castration-resistant Prostate CancerClinical cancer research : an official journal of the American Association for Cancer Research33504551Human T cells were obtained from AllCells. Human peripheral blood mononuclear cells (PBMC) were isolated from buffy coats collected at the Institute of Clinical Transfusion Medicine SaveFull Paper
2021Giffin, MJ;Cooke, K;Lobenhofer, EK;Estrada, J;Zhan, J;Deegen, P;Thomas, M;Murawsky, CM;Werner, J;Liu, S;Lee, F;Homann, O;Friedrich, M;Pearson, JT;Raum, T;Yang, Y;Caenepeel, S;Stevens, J;Beltran, PJ;Canon, J;Coxon, A;Bailis, JM;Hughes, PE;AMG 757, a Half-Life Extended, DLL3-Targeted Bispecific T-Cell Engager, Shows High Potency and Sensitivity in Preclinical Models of Small-Cell Lung CancerClinical cancer research : an official journal of the American Association for Cancer Research33203642human CD3+ T cells (2 × 107) by intravenous infusion on study day 0. Human CD3+ T cells, isolated from PBMCs from two different donors using negative selection, were purchased from AllCells and activated in vitro using IL2 and CD3/CD28/CD2 (STEMCELLFull Paper
2021Kögler, AC;Kherdjemil, Y;Bender, K;Rabinowitz, A;Marco-Ferreres, R;Furlong, EEM;Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryosDevelopmental cell34363757were also taken from regions not overlapping any mCherry-expressing cells (background (BG)) and from regions overlapping the entire group of cells investigated (AllCells) to Save;each time point, the same measurements were also taken from regions nFull Paper
2021Montalban-Bravo, G;Kanagal-Shamanna, R;Darbaniyan, F;Siddiqui, MT;Sasaki, K;Wei, Y;Yang, H;Chien, KS;Naqvi, K;Jabbour, E;Kadia, TM;Daver, N;DiNardo, C;Ravandi, F;Pemmaraju, N;Bose, P;Verstovsek, S;Pierce, S;Bueso-Ramos, C;Patel, K;Do, KA;Kantarjian, H;Garcia-Manero, G;Clinical, genomic, and transcriptomic differences between myelodysplastic syndrome/myeloproliferative neoplasm with ring sideroblasts and thrombocytosis (MDS/MPN-RS-T) and myelodysplastic syndrome with ring sideroblasts (MDS-RS)American journal of hematology33811786seven patients with MDS-RS, and 17 healthy individuals obtained from AllCells (Emeryville, CA) were isolated using the CD34 MicroBead Kit and RNA was isolated using the PicoPure RNA isolation kit, following manufacturer's instructions. Fastq files weFull Paper
2021Kwee, BJ;Lam, J;Akue, A;KuKuruga, MA;Zhang, K;Gu, L;Sung, KE;Functional heterogeneity of IFN-γ-licensed mesenchymal stromal cell immunosuppressive capacity on biomaterialsProceedings of the National Academy of Sciences of the United States of America34446555MSCs obtained from AllCells and Lonza cultured on the two hydrogels (SI Appendix Overall similar observations were obtained with a representative MSC line obtained from AllCells ( SaveFull Paper
2021Cui, L;Moraga, I;Lerbs, T;Van Neste, C;Wilmes, S;Tsutsumi, N;Trotman-Grant, AC;Gakovic, M;Andrews, S;Gotlib, J;Darmanis, S;Enge, M;Quake, S;Hitchcock, IS;Piehler, J;Garcia, KC;Wernig, G;Tuning MPL signaling to influence hematopoietic stem cell differentiation and inhibit essential thrombocythemia progenitorsProceedings of the National Academy of Sciences of the United States of America33384332Healthy deidentified human bone marrow samples were acquired from AllCells. Essential thrombocytosis bone marrow samples were deidentified and acquired from the Stanford SaveFull Paper
2021Poussin, M;Sereno, A;Wu, X;Huang, F;Manro, J;Cao, S;Carpenito, C;Glasebrook, A;Powell, DJ;Demarest, S;Dichotomous impact of affinity on the function of T cell engaging bispecific antibodiesJournal for immunotherapy of cancer34253637w cytometric evaluation of cell surface proteins on T cells 48 hours after mixing SKOV3 cells and biologic test articles is further described in online supplemental methods. Briefly, human T cells (ALLCELLS frozen normal human peripheral blood CD3+ PFull Paper
2021Roselli, E;Boucher, JC;Li, G;Kotani, H;Spitler, K;Reid, K;Cervantes, EV;Bulliard, Y;Tu, N;Lee, SB;Yu, B;Locke, FL;Davila, ML;4-1BB and optimized CD28 co-stimulation enhances function of human mono-specific and bi-specific third-generation CAR T cellsJournal for immunotherapy of cancer34706886Human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells. T cells were enriched from PBMCs using the EasySep human T cell isolation kit according to SaveFull Paper
2021Paavola, KJ;Roda, JM;Lin, VY;Chen, P;O'Hollaren, KP;Ventura, R;Crawley, SC;Li, B;Chen, HH;Malmersjö, S;Sharkov, NA;Horner, G;Guo, W;Kutach, AK;Mondal, K;Zhang, Z;Lichtman, JS;Song, C;Rivera, LB;Liu, W;Luo, J;Wang, Y;Solloway, MJ;Allan, BB;Kekatpure, A;Starck, SR;Haldankar, R;Fan, B;Chu, C;Tang, J;Molgora, M;Colonna, M;Kaplan, DD;Hsu, JY;The Fibronectin-ILT3 Interaction Functions as a Stromal Checkpoint that Suppresses Myeloid CellsCancer immunology research34426457Human peripheral blood mononuclear cells (PBMC) of healthy donors were isolated from freshly drawn leukopaks (Allcells, catalog no. PB007) by density gradient centrifugation over SaveFull Paper
2021Badeaux, MD;Rolig, AS;Agnello, G;Enzler, D;Kasiewicz, MJ;Priddy, L;Wiggins, JF;Muir, A;Sullivan, MR;Van Cleef, J;Daige, C;Vander Heiden, MG;Rajamanickam, V;Wooldridge, JE;Redmond, WL;Rowlinson, SW;Arginase Therapy Combines Effectively with Immune Checkpoint Blockade or Agonist Anti-OX40 Immunotherapy to Control Tumor GrowthCancer immunology research33500272Primary human bone marrow (AllCells) was obtained from healthy patients and cultured as follows. Briefly, Hs5 cells were cultured for 72 hours in SFEM2 media (Stem Cell SaveFull Paper
2021Boucher, JC;Li, G;Kotani, H;Cabral, ML;Morrissey, D;Lee, SB;Spitler, K;Beatty, NJ;Cervantes, EV;Shrestha, B;Yu, B;Kazi, A;Wang, X;Sebti, SM;Davila, ML;CD28 Costimulatory Domain-Targeted Mutations Enhance Chimeric Antigen Receptor T-cell FunctionCancer immunology research33188139An obstacle to the development of chimeric antigen receptor (CAR) T cells is the limited understanding of CAR T-cell biology and the mechanisms behind their antitumor activity. We and SaveFull Paper
2021Li, J;Whelan, S;Kotturi, MF;Meyran, D;D'Souza, C;Hansen, K;Liang, S;Hunter, J;Trapani, JA;Neeson, PJ;PVRIG is a novel natural killer cell immune checkpoint receptor in acute myeloid leukemiaHaematologica33147937nt clinical characteristics are summarised in the Online Supplementary Table S2. Healthy donor bone marrow samples were obtained from Royal Melbourne Hospital (Melbourne, Australia) or purchased from AllCells (Alameda, California). All bone marrow saFull Paper
2021Gilmartin, AG;Groy, A;Gore, ER;Atkins, C;Long, ER;Montoute, MN;Wu, Z;Halsey, W;McNulty, DE;Ennulat, D;Rueda, L;Pappalardi, M;Kruger, RG;McCabe, MT;Raoof, A;Butlin, R;Stowell, A;Cockerill, M;Waddell, I;Ogilvie, D;Luengo, J;Jordan, A;Benowitz, AB;In vitro and in vivo induction of fetal hemoglobin with a reversible and selective DNMT1 inhibitorHaematologica32586904Cryopreserved human bone marrow CD34+ cells (AllCells) were confirmed to be sourced ethically, and their research use was in accord with the terms of the informed consents under SaveFull Paper
2021Zhang, C;Zhang, X;Huang, L;Guan, Y;Huang, X;Tian, XL;Zhang, L;Tao, W;ATF3 drives senescence by reconstructing accessible chromatin profilesAging cell33539668accessibility in IARs. Accordingly, our research provides a target to aim for in the exploration of senescence and aging. 4 EXPERIMENTAL PROCEDURES 4.1 Cell culture We purchased primary HUVECs from AllCells Biotech Shanghai Co., Ltd., and cultured tFull Paper
2021Nesterenko, PA;McLaughlin, J;Tsai, BL;Burton Sojo, G;Cheng, D;Zhao, D;Mao, Z;Bangayan, NJ;Obusan, MB;Su, Y;Ng, RH;Chour, W;Xie, J;Li, YR;Lee, D;Noguchi, M;Carmona, C;Phillips, JW;Kim, JT;Yang, L;Heath, JR;Boutros, PC;Witte, ON;HLA-A∗02:01 restricted T cell receptors against the highly conserved SARS-CoV-2 polymerase cross-react with human coronavirusesCell reports34919800Cryo preserved peripheral blood mononuclear cells (PBMCs) were commercially purchased (Allcells and Hemacare) or obtained from the CFAR Virology Core Laboratory at the UCLA Save;d virus strains ------------------------- NFAT-zsGreen virus UCLA,Full Paper
2021Qian, Y;Arellano, G;Ifergan, I;Lin, J;Snowden, C;Kim, T;Thomas, JJ;Law, C;Guan, T;Balabanov, RD;Kaech, SM;Miller, SD;Choi, J;ZEB1 promotes pathogenic Th1 and Th17 cell differentiation in multiple sclerosisCell reports34433042FN-γ, 1 μg/mL anti-IL2 (Cat #554375, clone: S4B6, BD). Isolation of human CD4+ naive T cells and Th cells differentiation Fresh Leukopak samples from de-identified blood donors were purchased from AllCells, LLC. Peripheral blood mononuclear cells (PFull Paper
2021Hamilton, JR;Tsuchida, CA;Nguyen, DN;Shy, BR;McGarrigle, ER;Sandoval Espinoza, CR;Carr, D;Blaeschke, F;Marson, A;Doudna, JA;Targeted delivery of CRISPR-Cas9 and transgenes enables complex immune cell engineeringCell reports34077734ons. Isolation and culture of human primary T cells Primary adult blood cells were obtained from anonymous healthy human donors as a leukoreduction pack purchased from StemCell Technologies, Inc. or Allcells Inc, or as a Trima residual from VitalantFull Paper
2021Jiang, Q;Isquith, J;Ladel, L;Mark, A;Holm, F;Mason, C;He, Y;Mondala, P;Oliver, I;Pham, J;Ma, W;Reynoso, E;Ali, S;Morris, IJ;Diep, R;Nasamran, C;Xu, G;Sasik, R;Rosenthal, SB;Birmingham, A;Coso, S;Pineda, G;Crews, L;Donohoe, ME;Venter, JC;Whisenant, T;Mesa, RA;Alexandrov, LB;Fisch, KM;Jamieson, C;Inflammation-driven deaminase deregulation fuels human pre-leukemia stem cell evolutionCell reports33503434Aged normal and MPN Patient samples Obtained through patients consenting at UCSD Moores Cancer Center according to Institutional Review Boardapproved protocols. N/A Young Cord blood CD34+ cells Purchased from AllCells or StemCell TechnologiesFull Paper
2021Bunis, DG;Bronevetsky, Y;Krow-Lucal, E;Bhakta, NR;Kim, CC;Nerella, S;Jones, N;Mendoza, VF;Bryson, YJ;Gern, JE;Rutishauser, RL;Ye, CJ;Sirota, M;McCune, JM;Burt, TD;Single-Cell Mapping of Progressive Fetal-to-Adult Transition in Human Naive T CellsCell reports33406429Matched adult peripheral blood and BM samples were obtained from healthy volunteer donors through AllCells (Alameda, CA). UCB and longitudinal post-natal samples were obtained SaveFull Paper
2021Yang, Z;Huang, Y;Zhu, L;Yang, K;Liang, K;Tan, J;Yu, B;SIRT6 promotes angiogenesis and hemorrhage of carotid plaque via regulating HIF-1α and reactive oxygen speciesCell death & disease33436551oCl2) was purchased from Sigma-Aldrich, Inc. (Burlington, MA, USA). The work concentration and time were confirmed by instructions combined with experimental requirements. HUVECs were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured inFull Paper
2021Yang, Z;Wei, X;Pan, Y;Xu, J;Si, Y;Min, Z;Yu, B;A new risk factor indicator for papillary thyroid cancer based on immune infiltrationCell death & disease33414407d. The sections were fixed and images were obtained with an inverted microscope (Olympus IX71, Japan). Cell culture and co-culture Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured inFull Paper
2021Everitt, ML;Boegner, DJ;Birukov, KG;White, IM;Sample-to-Answer Diagnostic System for the Detection of Circulating Histones in Whole BloodACS sensors34270219Varying concentrations of histone octamers (Sigma-Aldrich) (0-65 μg/mL final concentrations) are suspended in either fetal bovine serum (FBS) (ATCC) or whole blood (AllCells) (medium is indicated in figure caption). Fifteen microliters of histone-spiFull Paper
2021Varricchio, L;Iancu-Rubin, C;Upadhyaya, B;Zingariello, M;Martelli, F;Verachi, P;Clementelli, C;Denis, JF;Rahman, AH;Tremblay, G;Mascarenhas, J;Mesa, RA;O'Connor-McCourt, M;Migliaccio, AR;Hoffman, R;TGF-β1 protein trap AVID200 beneficially affects hematopoiesis and bone marrow fibrosis in myelofibrosisJCI insight34383713n and response to TGF-β. Methods Further information and complete unedited blots can be found in Supplemental Methods. Human subjects. Primary ND specimens were purchased from a commercial source (AllCells, LLC), whereas MF MNCs were provided by thFull Paper
2021Pan, Y;You, Y;Sun, L;Sui, Q;Liu, L;Yuan, H;Chen, C;Liu, J;Wen, X;Dai, L;Sun, H;The STING antagonist H-151 ameliorates psoriasis via suppression of STING/NF-κB-mediated inflammationBritish journal of pharmacology34460100Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells Shanghai and were used immediately after resuscitation. H-151 was synthesized according to the published SaveFull Paper
2021Hu, X;Qie, C;Jiang, J;Xie, X;Chen, W;Liu, W;Liu, J;M351-0056 is a novel low MW compound modulating the actions of the immune-checkpoint protein VISTABritish journal of pharmacology33450048Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells and were used immediately after resuscitation; these cells cannot be subcultured. Human CD4 + T cells SaveFull Paper
2021Wang, X;Guo, J;Wen, J;Zhang, X;Cao, L;Zeng, D;Liu, X;Jiang, X;Novel vascular strategies on polyetheretherketone modification in promoting osseointegration in ovariectomized ratsMaterials & DesignHUVECs were purchased from AllCells Biology Technology Company (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, Lonza) without endothelial cell growth supplementation. HUVECs at a density of 2 × 104 cells per mL were seeded oFull Paper
2021Iftakhar-E-Khuda, I;Pessia, A;Zheng, S;Kankainen, M;Kontro, M;Karikoski, M;Laurila, J;Gerke, H;Tadayon, S;Hollmén, M;Tang, J;Imhof, BA;Salmi, M;Jalkanen, S;Vascular adhesion protein-1 defines a unique subpopulation of human hematopoietic stem cells and regulates their proliferationCellular and molecular life sciences : CMLS34719737Five hundred cryopreserved and thawed human BM CD34 + cells (AllCells) were cultured in complete methylcellulose-based medium (H4436, STEMCELL Technologies) with or SaveFull Paper
2021Huang, YJ;Cao, J;Lee, CY;Wu, YM;Umbilical cord blood plasma-derived exosomes as a novel therapy to reverse liver fibrosisStem cell research & therapy34772443Human UCB and peripheral blood (PB) plasma were purchased from AllCells LLC (Alameda, CA), and exosomes were acquired using ExoQuick Exosome isolation reagent (System SaveFull Paper
2021Pieles, O;Reichert, TE;Morsczeck, C;Classical isoforms of protein kinase C (PKC) and Akt regulate the osteogenic differentiation of human dental follicle cells via both β-catenin and NF-κBStem cell research & therapy33853677to examine the role of classical PKC isoforms and the potential downstream mechanisms during the osteogenic differentiation of DFCs. Materials and methods Cell culture Human DFCs were purchased from AllCells (Emeryville, USA) and grown in Dulbecco’sFull Paper
2021Lin, Z;Liu, X;Liu, T;Gao, H;Wang, S;Zhu, X;Rong, L;Cheng, J;Cai, Z;Xu, F;Tan, X;Lv, L;Li, Z;Sun, Y;Qian, Q;Evaluation of Nonviral piggyBac and lentiviral Vector in Functions of CD19chimeric Antigen Receptor T Cells and Their Antitumor Activity for CD19+ Tumor CellsFrontiers in immunology35082789Peripheral blood mononuclear cells (PBMCs) from whole blood were purchased from AllCells (Shanghai, China) and isolated by Ficoll density gradient centrifugation (Cat#17-5442-03, SaveFull Paper
2021Cui, X;Jia, H;Xin, H;Zhang, L;Chen, S;Xia, S;Li, X;Xu, W;Chen, X;Feng, Y;Wei, X;Yu, H;Wang, Y;Zhan, Y;Zhu, X;Zhang, X;A Novel Bispecific Antibody Targeting PD-L1 and VEGF With Combined Anti-Tumor ActivitiesFrontiers in immunology34925354Human PBMCs and DCs were acquired by using the Easy MLR Kit (Allcells, Alameda). DCs were treated with mitomycin (H19999025, Hisun Pharm Co., Ltd.) for 30 min before co- SaveFull Paper
2021Ceglia, V;Kelley, EJ;Boyle, AS;Zurawski, S;Mead, HL;Harms, CE;Blanck, JP;Flamar, AL;Kirschman, JH;Ogongo, P;Ernst, JD;Levy, Y;Zurawski, G;Altin, JA;A Framework to Identify Antigen-Expanded T Cell Receptor Clusters Within Complex RepertoiresFrontiers in immunology34917073cificity, are described in other studies (16, 17, 19). Cohesin-Influenza Matrix 1 (Flu M1) protein is described in (16). Donors Cryopreserved human PBMC from normal donors were sourced commercially (AllCells, CA. ND1001 ID:A5983, ND1002 ID:9441, ND1Full Paper
2021Teo Hansen Selnø, A;Schlichtner, S;Yasinska, IM;Sakhnevych, SS;Fiedler, W;Wellbrock, J;Berger, SM;Klenova, E;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV;High Mobility Group Box 1 (HMGB1) Induces Toll-Like Receptor 4-Mediated Production of the Immunosuppressive Protein Galectin-9 in Human Cancer CellsFrontiers in immunology34234778Medical Centre Hamburg-Eppendorf (Ethik Kommission der Ärztekammer Hamburg, reference: PV3469). Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were also purchased from AllCells (Alameda, CA, USA) and handled in accordancFull Paper
2021Xie, X;Chen, C;Chen, W;Jiang, J;Wang, L;Li, T;Sun, H;Liu, J;Structural Basis of VSIG3: The Ligand for VISTAFrontiers in immunology33841409azole buffer (GenScript). Protein was concentrated using 10K MWCO spin columns (Merck). The purified protein was used for functional verification. Cell Culture and Reagents PBMCs were purchased from Allcells Biotechnology (Shanghai) Co., LTD, and weFull Paper
2021Jost, M;Jacobson, AN;Hussmann, JA;Cirolia, G;Fischbach, MA;Weissman, JS;CRISPR-based functional genomics in human dendritic cellseLife33904395t al., 2018 Referred to as ‘B. theta 4PP’ in the text Mutant producing tetra-acylated, di-phosphorylated lipid A in a capsule-producing background Biological sample (human) PBMCs AllCells Freshly isolated from de-identified healtFull Paper
2021Gao, X;Guan, M;Liu, X;Xu, H;Huang, Q;Chen, L;Huang, S;Xiao, Y;Shi, X;Lin, Z;Sustained delivery of growth factors and alendronate using partially demineralized dentin matrix for endogenous periodontal regenerationApplied Materials Todaycells (HUVECs) were purchased from AllCells (Shanghai, China). HUVECs were cultured in endothelial cell growth medium (EGM; AllCells, Shanghai, China). Angiogenesis SaveFull Paper
2021Scherer, SD;Riggio, AI;Haroun, F;DeRose, YS;Ekiz, HA;Fujita, M;Toner, J;Zhao, L;Li, Z;Oesterreich, S;Samatar, AA;Welm, AL;An immune-humanized patient-derived xenograft model of estrogen-independent, hormone receptor positive metastatic breast cancerBreast cancer research : BCR34717714CD34 + hematopoietic stem cells (HSCs) isolated from healthy donor bone marrow (BM) were purchased from AllCells. On the day of injection, cells were thawed in a 37 C water bath SaveFull Paper
2021Nada, MH;Wang, H;Hussein, AJ;Tanaka, Y;Morita, CT;PD-1 checkpoint blockade enhances adoptive immunotherapy by human Vγ2Vδ2 T cells against human prostate cancerOncoimmunology34712512e IgG1 (clone MOPC-21) antibodies were from Bio X Cell (West Lebanon, NH). Ex vivo expansion of Vγ2Vδ2 T cells by pulse zoledronic acid stimulation PBMC were isolated from random donor leukopaks (AllCells, LLC, Alameda, CA) by density centrifugationFull Paper
2021Jiang, H;Ni, H;Zhang, P;Guo, X;Wu, M;Shen, H;Wang, J;Wu, W;Wu, Z;Ding, J;Tang, R;Zhou, S;Chen, B;Yu, M;Jing, H;Liu, J;PD-L1/LAG-3 bispecific antibody enhances tumor-specific immunityOncoimmunology34239776oduced by Innovent Biologics Co., Ltd. (Suzhou, China). Human IgG was purchased from Equitech-Bio (Kerrville). Peripheral blood mononuclear cells (PBMCs) and dendritic cells (DCs) were purchased from AllCells (Shanghai, China). Cynomolgus macaque PBMFull Paper
2021Wang, X;Peticone, C;Kotsopoulou, E;Göttgens, B;Calero-Nieto, FJ;Single-cell transcriptome analysis of CAR T-cell products reveals subpopulations, stimulation, and exhaustion signaturesOncoimmunology33489472or samples PBMCs were derived from healthy donors leukapheresis. Peripheral blood leukapaheresis were obtained from the NHS as part of a research study (IRAS ID 185945) or purchased as LeukoPaks from AllCells. Manufacturing of CAR T cells product GeFull Paper
2021Zhang, C;Delawary, M;Huang, P;Korchak, JA;Suda, K;Zubair, AC;IL-10 mRNA Engineered MSCs Demonstrate Enhanced Anti-Inflammation in an Acute GvHD ModelCells34831324g T7 promoter, 5′ UTRs, 3′ UTRs, and poly-A tail. 2.3. MSC Culture and Time Course Experiment Human MSCs were generated from commercial de-identified bone marrow (Catalog# ABM002) purchased from AllCells (Alameda, CA, USA). The donor was a healthy 2Full Paper
2021Ahn, Y;An, JH;Yang, HJ;Lee, DG;Kim, J;Koh, H;Park, YH;Song, BS;Sim, BW;Lee, HJ;Lee, JH;Kim, SU;Human Blood Vessel Organoids Penetrate Human Cerebral Organoids and Form a Vessel-Like SystemCells34440805Human Induced Pluripotent Stem Cells (hiPSCs) and Culture Conditions The hiPSCs were reprogrammed from primary human bone marrow cells (Allcells, Emeryville, CA, USA) as Save;The hiPSCs were reprogrammed from primary human bone marrow cells (AllcelFull Paper
2021Dogan, F;Aljumaily, RMK;Kitchen, M;Forsyth, NR;DNMT3B Is an Oxygen-Sensitive De Novo Methylase in Human Mesenchymal Stem CellsCells33925659nd Methods 2.1. Isolation and Culture of BM-hMSCs Three human BM-hMSCs BMA-16 (Male, Age—29 years, Lonza, MD, USA), BMA-20 (Female, Age—50 years, Lonza, MD, USA) and BMA-25 (Male, Age—27 years, AllCells, LLC, Alameda, CA, USA) were isolated from commFull Paper
2021Miao, G;Sun, X;Development of a novel anti-B7-H4 antibody enhances anti-tumor immune response of human T cellsBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapie34328096Human PBMCs were purchased from ALLCELLS. Hybridoma cell lines were cultured in H-SFM complete medium. CD8+ T cells were cultured in RPMI 1640 medium containing 2 mM L- SaveFull Paper
2021Gu, C;Cao, X;Wang, Z;Hu, X;Yao, Y;Zhou, Y;Liu, P;Liu, X;Gao, G;Hu, X;Zhang, Y;Chen, Z;Gao, L;Peng, Y;Jia, F;Shan, C;Yu, L;Liu, K;Li, N;Guo, W;Jiang, G;Min, J;Zhang, J;Yang, L;Shi, M;Hou, T;Li, Y;Liang, W;Lu, G;Yang, C;Wang, Y;Xia, K;Xiao, Z;Xue, J;Huang, X;Chen, X;Ma, H;Song, D;Pan, Z;Wang, X;Guo, H;Liang, H;Yuan, Z;Guan, W;Deng, SJ;A human antibody of potent efficacy against SARS-CoV-2 in rhesus macaques showed strong blocking activity to B.1.351mAbs34097570ed SARS-CoV-2 RBD (Sino Biological, 40592-V05H). Phage libraries were constructed using antibody gene fragments amplified from peripheral blood mononuclear cells (PBMCs) of 50 healthy human subjects (Allcells, PB003F and PB003 C) and total RNA from PFull Paper
2021Chen, J;Ding, J;Wu, Y;Zhang, S;Zheng, N;Yang, J;Xu, J;Chromium Oxide Nanoparticle Impaired Osteogenesis and Cellular Response to Mechanical StimulusInternational journal of nanomedicine34511912Human MSCs were isolated from bone marrow aspirate (ALLCELLS, Lot No: BM2893). The cells were grown in α-MEM (Gibco, Thermo Fisher Scientific, UK) supplemented with 10% SaveFull Paper
2021Shin, Y;Park, CM;Kim, HG;Kim, DE;Choi, MS;Kim, JA;Choi, BS;Yoon, CH;Identification of Aristolactam Derivatives That Act as Inhibitors of Human Immunodeficiency Virus Type 1 Infection and Replication by Targeting Tat-Mediated Viral TranscriptionVirologica Sinica32779073Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Alameda, CA, USA) and cultured, as described previously (Yoon et al. 2015). HIV-1 clones pNL4-3 and AD8, as well as TZM-bl and A3.01 cells, were obtained from the National InstiFull Paper
2021Wang, Y;Ni, H;Zhou, S;He, K;Gao, Y;Wu, W;Wu, M;Wu, Z;Qiu, X;Zhou, Y;Chen, B;Pan, D;Huang, C;Li, M;Bian, Y;Yang, M;Miao, L;Liu, J;Tumor-selective blockade of CD47 signaling with a CD47/PD-L1 bispecific antibody for enhanced anti-tumor activity and limited toxicityCancer immunology, immunotherapy : CII32761423CD4+ T cells were isolated from human PBMC (AllCells) using EasySep human CD4+ T cells enrichment kit (StemCell Technologies). DCs were generated by incubating PBMC first SaveFull Paper
2021Luban, J;Sattler, RA;Mühlberger, E;Graci, JD;Cao, L;Weetall, M;Trotta, C;Colacino, JM;Bavari, S;Strambio-De-Castillia, C;Suder, EL;Wang, Y;Soloveva, V;Cintron-Lue, K;Naryshkin, NA;Pykett, M;Welch, EM;O'Keefe, K;Kong, R;Goodwin, E;Jacobson, A;Paessler, S;Peltz, SW;The DHODH inhibitor PTC299 arrests SARS-CoV-2 replication and suppresses induction of inflammatory cytokinesVirus research33249060c. Uridine, brequinar, and A77 were obtained from Sigma-Aldrich (St. Louis, MO). Anti IL-4 and anti-IFL-γ antibodies were obtained from BD Biosciences (San Jose, CA). Frozen PBMCs were obtained from AllCells (Alameda, CA). IL-17, IL-17 F and IL-23 ELFull Paper
2021Satani, N;Zhang, X;Giridhar, K;Wewior, N;Cai, C;Aronowski, J;Savitz, SI;A Combination of Atorvastatin and Aspirin Enhances the Pro-Regenerative Interactions of Marrow Stromal Cells and Stroke-Derived Monocytes In VitroFrontiers in pharmacology33959001d of stroke patients. Methods Isolation and Culture of Human Mesenchymal Stromal Cells (MSCs) MSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old male (AllCells, Alameda, CA) using density centrifugationFull Paper
2021Sun, LK;Engineering genetic thermal switches for remote control of therapeutic T cellsThesisPrimary human CD3+ T cells were obtained from anonymous donor blood following apheresis (AllCells) and were cryopreserved until subsequent use. Two days prior to lentiviral SaveFull Paper
2021Wu, HH;Ralph, KL;Sepuldeva, E;Hansen, G;Li, H;Huang, ZF;Liu, D;Dziegelewski, M;Ahlberg, J;Frego, L;Fogal, S;van Tongeren, S;Grimaldi, C;Litzenberger, T;Presky, D;Singh, S;Brodeur, S;Kroe-Barrett, R;An optimally designed anti-human CD40 antibody with potent B cell suppression for the treatment of autoimmune diseasesInternational journal of pharmaceutics34624444Effector cells were generated by stimulating human peripheral blood mononuclear cells (PBMCs) (AllCells) with 10 ng/mL human IL-2 (R&D Systems) in RPMI 1640 medium (Invitrogen) SaveFull Paper
2021Xiang, H;Chan, AG;Ahene, A;Bellovin, DI;Deng, R;Hsu, AW;Jeffry, U;Palencia, S;Powers, J;Zanghi, J;Collins, H;Preclinical characterization of bemarituzumab, an anti-FGFR2b antibody for the treatment of cancermAbs34719330the full-length human FGFR2b described as Ba/F3 FGFR2b, and the effector cells were peripheral blood mononuclear cells obtained fresh from individual human donors (AllCells; SaveFull Paper
2021Li, G;Guo, J;Zheng, Y;Ding, W;Han, Z;Qin, L;Mo, W;Luo, M;CXCR5 guides migration and tumor eradication of anti-EGFR chimeric antigen receptor T cellsMolecular therapy oncolytics34553036CAR-T cells were generated from commercial normal donor peripheral blood mononuclear cells (PBMCs) from AllCells. The cells were further enriched from PBMC by CD3 magnetic Save;.22-μm filter, aliquoted to 2 mL cryotubes, quick frozen on dry ice, anFull Paper
2021Chen, X;Yang, S;Li, S;Qu, Y;Wang, HY;Liu, J;Dunn, ZS;Cinay, GE;MacMullan, MA;Hu, F;Zhang, X;Wang, P;Secretion of bispecific protein of anti-PD-1 fused with TGF-β trap enhances antitumor efficacy of CAR-T cell therapyMolecular therapy oncolytics33981830Frozen human PBMCs were obtained from AllCells. The T cell culture medium (TCM) was composed of AIM-V medium with 5% (v/v) human AB serum, 10 mM HEPES, 1% (v/v) Save;ng gag-pol. The supernatants were harvested at 48 h after transfection and filtereFull Paper
2021Branquinho, MV;Ferreira, SO;Alvites, RD;Magueta, AF;Ivanov, M;Sousa, AC;Amorim, I;Faria, F;Fernandes, MHV;Vilarinho, PM;Maurício, AC;In Vitro and In Vivo Characterization of PLLA-316L Stainless Steel Electromechanical Devices for Bone Tissue Engineering-A Preliminary StudyInternational journal of molecular sciences34299274e enthalpy of fusion of 100% crystalline material [54]. (1)XC (%)=ΔHfΔHf0×100 4.3. In Vitro Assays 4.3.1. Cell Culture and Maintenance Human Dental Pulp stem/stromal cells (hDPSCs) obtained from AllCells, LLC, Alameda, CA, USA (Cat. DP0037F, Lot no.Full Paper
2021Thavapalachandran, S;Le, TYL;Romanazzo, S;Rashid, FN;Ogawa, M;Kilian, KA;Brown, P;Pouliopoulos, J;Barry, AM;Fahmy, P;Kelly, K;Kizana, E;Chong, JJH;Pluripotent stem cell-derived mesenchymal stromal cells improve cardiac function and vascularity after myocardial infarctionCytotherapy34588150Cells were kept cold on ice until administration to animals. BM-MSCs were purchased from AllCells (Alameda, CA, USA). Cryopreserved BM-MSCs were washed twice in DMEM and resuspended at the same density as Cymerus MSC derivatives (5 × 106 cells per 10Full Paper
2021Kavanagh, H;Dunne, S;Martin, DS;McFadden, E;Gallagher, L;Schwaber, J;Leonard, S;O'Dea, S;A novel non-viral delivery method that enables efficient engineering of primary human T cells for ex vivo cell therapy applicationsCytotherapy33941482cells with minimal perturbation of cell function. Methods Ethics All experimental methods were carried out in accordance with the approved guidelines. Leukopaks from healthy donors were sourced from AllCells (Quincy, MA, USA) or BioIVT (Westbury, NYFull Paper
2021Wang, Y;Zhong, K;Ke, J;Chen, X;Chen, Y;Shu, W;Chen, C;Hu, S;Sun, X;Huang, H;Luo, C;Liu, L;Yang, J;Zhang, Y;Zhi, H;Combined 4-1BB and ICOS co-stimulation improves anti-tumor efficacy and persistence of dual anti-CD19/CD20 chimeric antigen receptor T cellsCytotherapy33863641Human peripheral blood mononuclear cells (PBMCs) from healthy donors were obtained from AllCells. The cryopreserved PBMCs were thawed into the T-cell culture medium and SaveFull Paper
2021Martin, A;Daris, M;Johnston, JA;Cui, J;HLA-A*02:01-directed chimeric antigen receptor/forkhead box P3-engineered CD4+ T cells adopt a regulatory phenotype and suppress established graft-versus-host diseaseCytotherapy33309258A*02:01- peripheral blood mononuclear cells (PBMCs) from four donors were isolated from fresh Leukopak (AllCells) using Ficoll density gradient media. CD4+ T cells or Tregs were SaveFull Paper
2021Story, JY;Zoine, JT;Burnham, RE;Hamilton, JAG;Spencer, HT;Doering, CB;Raikar, SS;Bortezomib enhances cytotoxicity of ex vivo-expanded gamma delta T cells against acute myeloid leukemia and T-cell acute lymphoblastic leukemiaCytotherapy33168453PBMCs (50-100 million per donor) from six different healthy donors were obtained through AllCells ( Eight total expansions from PBMCs from AllCells were performed, with two separate SaveFull Paper
2021Banerjee, A;Lu, Y;Do, K;Mize, T;Wu, X;Chen, X;Chen, J;Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain DiseasesFrontiers in cellular neuroscience33897370ity for polarization. With whole-genome transcript profile, we also demonstrated that iMG cells were clustered closely with human microglia. Materials and Methods Materials PBMCs were purchased from AllCells, LLC (Alameda, CA, USA, #PB003F and #PB00Full Paper
2021Stem, C;Rodman, C;Ramamurthy, RM;George, S;Meares, D;Farland, A;Atala, A;Doering, CB;Spencer, HT;Porada, CD;Almeida-Porada, G;Investigating Optimal Autologous Cellular Platforms for Prenatal or Perinatal Factor VIII Delivery to Treat Hemophilia AFrontiers in cell and developmental biology34447745Bone marrow mononuclear cells from two different biological donors were purchased from AllCells (Alameda, CA, United States) and their purchase approved by the Office of Human SaveFull Paper
2021Han, Y;Chen, Q;Zhang, L;Dissanayaka, WL;Indispensable Role of HIF-1α Signaling in Post-implantation Survival and Angio-/Vasculogenic Properties of SHEDFrontiers in cell and developmental biology34368116e expression of target proteins that mediate adaptive metabolic mechanisms in both in vivo and in vitro survival and angiogenesis in SHED. Materials and Methods Cell Culture SHED were purchased from AllCells (Alameda, CA, United States) and culturedFull Paper
2021Yazawa, T;Inaba, H;Imamichi, Y;Sekiguchi, T;Uwada, J;Islam, MS;Orisaka, M;Mikami, D;Ida, T;Sato, T;Miyashiro, Y;Takahashi, S;Khan, MRI;Suzuki, N;Umezawa, A;Kitano, T;Profiles of 5α-Reduced Androgens in Humans and Eels: 5α-Dihydrotestosterone and 11-Ketodihydrotestosterone Are Active Androgens Produced in Eel GonadsFrontiers in endocrinology33833737healthy women volunteers (obtaining informed consent) at University of Fukui Hospital in 2007. Plasma was separated by centrifugation at 1000g for 5 minutes. Other plasma samples were purchased from AllCells (Alameda, CA, USA) and ProMedDX (Norton, MFull Paper
2021Sternjak, A;Lee, F;Thomas, O;Balazs, M;Wahl, J;Lorenczewski, G;Ullrich, I;Muenz, M;Rattel, B;Bailis, JM;Friedrich, M;Preclinical Assessment of AMG 596, a Bispecific T-cell Engager (BiTE) Immunotherapy Targeting the Tumor-specific Antigen EGFRvIIIMolecular cancer therapeutics33632870Human pan-T cells (AllCells) were added at an E:T ratio of 10:1 with a dose range of AMG 596. Microvesicles were added simultaneously with T cells and AMG 596 to maintain SaveFull Paper
2021Massimino, M;Tirrò, E;Stella, S;Manzella, L;Pennisi, MS;Romano, C;Vitale, SR;Puma, A;Tomarchio, C;Di Gregorio, S;Antolino, A;Di Raimondo, F;Vigneri, P;Impact of the Breakpoint Region on the Leukemogenic Potential and the TKI Responsiveness of Atypical BCR-ABL1 TranscriptsFrontiers in pharmacology34276365CD34-positive cells derived from healthy donors were obtained from ALLCELLS. CD34-positive cells were maintained in Stem Span SFEM supplemented with cytokines at low SaveFull Paper
2021Tang, J;Zou, Y;Li, L;Lu, F;Xu, H;Ren, P;Bai, F;Niedermann, G;Zhu, X;BAY 60-6583 Enhances the Antitumor Function of Chimeric Antigen Receptor-Modified T Cells Independent of the Adenosine A2b ReceptorFrontiers in pharmacology33776765ed States) + 10% FBS (10099141C, ThermoFisher Scientific) were used to expand and culture CAR T cells. Frozen human peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from AllCells (PB005F, Alameda, CA, United States). AftFull Paper
2021Sim, DS;Mallari, CR;Teare, JM;Feldman, RI;Bauzon, M;Hermiston, TW;In vitro characterization of CT-001-a short-acting factor VIIa with enhanced prohemostatic activityResearch and practice in thrombosis and haemostasis34263099e binding of FVIIa to activated platelet surfaces was detected by Alexa488‐anti‐FVII (clone AA3). Donor informed consent and collection protocols are approved by the Institutional Review Board of AllCells. 2.4 Inhibition by antithrombin and tissue fFull Paper
2021Xu, Y;Barauskas, O;Kim, C;Babusis, D;Murakami, E;Kornyeyev, D;Lee, G;Stepan, G;Perron, M;Bannister, R;Schultz, BE;Sakowicz, R;Porter, D;Cihlar, T;Feng, JY;Off-Target In Vitro Profiling Demonstrates that Remdesivir Is a Highly Selective Antiviral AgentAntimicrobial agents and chemotherapy33229429Normal human primary bone marrow (BM) light-density cells were from three different lots obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD). All primary human cells SaveFull Paper
2021Huang, Y;Jia, F;Zhao, J;Hou, Y;Hu, SQ;Novel ACE Inhibitory Peptides Derived from Yeast Hydrolysates: Screening, Inhibition Mechanisms and Effects on HUVECsJournal of agricultural and food chemistry33593053Primary HUVECs, as well as the endothelial growth medium (EGM) and endothelial basal serum-free medium, were purchased from Allcells Co., Ltd. (Shanghai, China). Penicillin/ SaveFull Paper
2021Jing, L;Fan, S;Yao, X;Zhang, Y;Effects of compound stimulation of fluid shear stress plus ultrasound on stem cell proliferation and osteogenesisRegenerative biomaterials34868635lopment kit was purchased from Beyotime Biotechnology Co., Ltd. (China). The MagneSil@ Total RNA mini isolation system was purchased from Promega (USA). Primary rat BMSCs were purchased from Shanghai AllCells Biotech Co., Ltd. (China). The polytetrafFull Paper
2021Chai, ZT;Zhang, XP;Ao, JY;Zhu, XD;Wu, MC;Lau, WY;Sun, HC;Cheng, SQ;AXL Overexpression in Tumor-Derived Endothelial Cells Promotes Vessel Metastasis in Patients With Hepatocellular CarcinomaFrontiers in oncology34123800Primary human umbilical vein endothelial cells (HUVECs; AllCells, Shanghai, China) were cultured in endothelial cell growth medium-2 (Lonza, Basel, Switzerland) supplemented with SaveFull Paper
2021Romito, M;Juillerat, A;Kok, YL;Hildenbeutel, M;Rhiel, M;Andrieux, G;Geiger, J;Rudolph, C;Mussolino, C;Duclert, A;Metzner, KJ;Duchateau, P;Cathomen, T;Cornu, TI;Preclinical Evaluation of a Novel TALEN Targeting CCR5 Confirms Efficacy and Safety in Conferring Resistance to HIV-1 InfectionBiotechnology journal33103367and data analyzed with CRISPResso2.[49] p values were adjusted with the Benjamini & Hochberg[50] method. For OCA, 1 × 106 PBMCs (ALLCELLS, USA)/mL in X-Vivo15 medium (Lonza), supplemented with 5% human AB serum (Gemini, USA) and 20 ng mL−1 of IL-2 (MFull Paper
2021Kitanaga, Y;Yamajuku, D;Kubo, S;Nakamura, K;Maeda, M;Seki, M;Kaneko, Y;Kinugasa, F;Morokata, T;Kondo, Y;Yoshinari, H;Nakayamada, S;Sumida, T;Tanaka, Y;Discovery of a novel Igβ and FcγRIIB cross-linking antibody, ASP2713, and its potential application in the treatment of systemic lupus erythematosusInternational immunopharmacology34781122Human peripheral blood mononuclear cells (PBMCs; Lonza, Basel, Switzerland) and normal human peripheral blood B cells (AllCells, CA, USA) were purchased. Whole blood samples were obtained from healthy subjects and patients with SLE at the UniversityFull Paper
2021Chen, YL;Cui, Y;Liu, X;Liu, G;Dong, X;Tang, L;Hung, Y;Wang, C;Feng, MQ;A bispecific antibody targeting HER2 and PD-L1 inhibits tumor growth with superior efficacyThe Journal of biological chemistry34798072man IgG (H + L) was obtained from Jackson ImmunoResearch. Monocyte purification kit was obtained from Miltenyi Biotec. Ficoll gradient was obtained from GE healthcare. Human PBMCs were obtained from Allcells. Humanization of anti-PD-L1 mAb by framewFull Paper
2021Langrish, CL;Bradshaw, JM;Francesco, MR;Owens, TD;Xing, Y;Shu, J;LaStant, J;Bisconte, A;Outerbridge, C;White, SD;Hill, RJ;Brameld, KA;Goldstein, DM;Nunn, PA;Preclinical Efficacy and Anti-Inflammatory Mechanisms of Action of the Bruton Tyrosine Kinase Inhibitor Rilzabrutinib for Immune-Mediated DiseaseJournal of immunology (Baltimore, Md. : 1950)33674445Human whole blood from healthy donors was purchased from AllCells and assayed for rilzabrutinib inhibition of IgE basophil activation. Whole blood was treated with ten 3-fold dilutions SaveFull Paper
2021Andrejeva, G;Capoccia, BJ;Hiebsch, RR;Donio, MJ;Darwech, IM;Puro, RJ;Pereira, DS;Novel SIRPα Antibodies That Induce Single-Agent Phagocytosis of Tumor Cells while Preserving T CellsJournal of immunology (Baltimore, Md. : 1950)33431660were differentiated from CD14+ monocytes purchased from Astarte Biologics or selected using Pan Monocyte Isolation Kit (Miltenyi Biotec) from PBMCs (AllCells and Hemacare). Monocytes were seeded onto 96-well, flat-bottom plates at 3 × 104 cells per wFull Paper
2021Chaerkady, R;Zhou, Y;Delmar, JA;Weng, SHS;Wang, J;Awasthi, S;Sims, D;Bowen, MA;Yu, W;Cazares, LH;Sims, GP;Hess, S;Characterization of Citrullination Sites in Neutrophils and Mast Cells Activated by Ionomycin via Integration of Mass Spectrometry and Machine LearningJournal of proteome research34008986For mast cell preparation, three donors were used. Briefly, leukocytes were collected from Leukopak (ALLCells) and mixed with Robo II Buffer (StemCell Technologies) at 1:4 and centrifuged at 500g for 15 mins. The pellet was resuspended in 1× VitalyseFull Paper
2021Deng, M;Wu, D;Zhang, Y;Jin, Z;Miao, J;MiR-29c downregulates tumor-expressed B7-H3 to mediate the antitumor NK-cell functions in ovarian cancerGynecologic oncology33875234The human CD56 + NK cells were obtained from ALLCELLS (Emeryville, California) and were confirmed by flow cytometric analyses. NK cells were maintained in Human NK cell SaveFull Paper
2021Friedman, D;Dozor, AJ;Milner, J;D'Souza, M;Talano, JA;Moore, TB;Shenoy, S;Shi, Q;Walters, MC;Vichinsky, E;Parsons, SK;Braniecki, S;Moorthy, CR;Ayello, J;Flower, A;Morris, E;Mahanti, H;Fabricatore, S;Klejmont, L;van de Ven, C;Baxter-Lowe, LA;Cairo, MS;Stable to improved cardiac and pulmonary function in children with high-risk sickle cell disease following haploidentical stem cell transplantationBone marrow transplantation33958740Bioverativ, Sangamo, Veevo, Editas Medicine and is medical director for AllCells, Inc. Biosciences. SKP is a consultant to Seattle Genetics, also unrelated to this study. EV is a consultant for ApoPharma, on the advisory committee for Global Blood ThFull Paper
2021Song, D;Liu, X;Dong, C;Wang, Q;Sha, C;Liu, C;Ning, Z;Han, J;Liu, H;Zong, M;Zhao, Y;Li, Y;Liu, G;Shao, X;Dou, C;Two novel human anti-CD25 antibodies with antitumor activity inversely related to their affinity and in vitro activityScientific reports34824364The effector cells PBMCs (PB003F-C, ALLCELLS) were added and the mixture was incubated at 37 C for 5 h. After substrate was added, OD490 value was read on the microplate Save;The effector cells PBMCs (PB003F-C, ALLCELLS) were added and the mixtureFull Paper
2021Loo, J;Sicher, I;Goff, A;Kim, O;Clary, N;Alexeev, A;Sulchek, T;Zamarayeva, A;Han, S;Calero-Garcia, M;Microfluidic transfection of mRNA into human primary lymphocytes and hematopoietic stem and progenitor cells using ultra-fast physical deformationsScientific reports34725429Mobilized peripheral blood CD34-selected hematopoietic stem and progenitor cells (mPB CD34 + HSPCs, purchased from AllCells) were taken from liquid nitrogen freezer and placed SaveFull Paper
2021Wang, X;Negrou, E;Maloney, MT;Bondar, VV;Andrews, SV;Montalban, M;Llapashtica, C;Maciuca, R;Nguyen, H;Solanoy, H;Arguello, A;Przybyla, L;Moerke, NJ;Huntwork-Rodriguez, S;Henry, AG;Understanding LRRK2 kinase activity in preclinical models and human subjects through quantitative analysis of LRRK2 and pT73 Rab10Scientific reports34145320University of Dundee (https://mrcppureagents.dundee.ac.uk/). For the total Rab10 assay, recombinant Rab10 protein (LSBio, LS-G2178) and Rab8 protein (LS-G29310) were used. Human cryopreserved PBMCs (AllCells, PB005F) were thawed and cultured in RPMIFull Paper
2021Xue, X;De Leon-Tabaldo, A;Luna-Roman, R;Castro, G;Albers, M;Schoetens, F;DePrimo, S;Devineni, D;Wilde, T;Goldberg, S;Kinzel, O;Hoffmann, T;Fourie, AM;Thurmond, RL;Preclinical and clinical characterization of the RORγt inhibitor JNJ-61803534Scientific reports34040108mentary materials and methods). Human nTreg suppressive assay Frozen purified human CD4+CD25+ natural Treg cells (nTreg), monocyte-derived dendritic cells (DC) and CD4+CD25- T effector cells (Teff) (Allcells, LLC, Alameda, CA) were thawed and co-culFull Paper
2021Harris, KE;Lorentsen, KJ;Malik-Chaudhry, HK;Loughlin, K;Basappa, HM;Hartstein, S;Ahmil, G;Allen, NS;Avanzino, BC;Balasubramani, A;Boudreau, AA;Chang, K;Cuturi, MC;Davison, LM;Ho, DM;Iyer, S;Rangaswamy, US;Sankaran, P;Schellenberger, U;Buelow, R;Trinklein, ND;A bispecific antibody agonist of the IL-2 heterodimeric receptor preferentially promotes in vivo expansion of CD8 and NK cellsScientific reports34011961resuspended in 125 µL/well flow buffer and acquired on a BD FACSCelesta. Whole blood cytokine release assay Cytokine secretion was detected using fresh human whole blood (heparinized) obtained from AllCells. The following method was adapted from B.Full Paper
2021Tago, Y;Kobayashi, C;Ogura, M;Wada, J;Yamaguchi, S;Yamaguchi, T;Hayashi, M;Nakaishi, T;Kubo, H;Ueda, Y;Human amnion-derived mesenchymal stem cells attenuate xenogeneic graft-versus-host disease by preventing T cell activation and proliferationScientific reports33510297Human BMSC from healthy donors were purchased from AllCells (CA, USA). The culture conditions of AMSC and BMSC (ie, MSC) were determined based on previous studies 43 . In SaveFull Paper
2021Subhi, H;Husein, A;Mohamad, D;Nik Abdul Ghani, NR;Nurul, AA;Chitosan-Based Accelerated Portland Cement Promotes Dentinogenic/Osteogenic Differentiation and Mineralization Activity of SHEDPolymers34641172SHED employed in this study were obtained from ALLCELLS, USA (Catalog No. DP004F; lot number SHED092211-01). Upon being received, the cells were characterized using stem SaveFull Paper
2021Kim, OH;Park, JH;Son, JI;Yoon, OJ;Lee, HJ;Bone Marrow Mesenchymal Stromal Cells on Silk Fibroin Scaffolds to Attenuate Polymicrobial Sepsis Induced by Cecal Ligation and PuncturePolymers33946773y (FE-SEM; JSM 7500F, JEOL Ltd., Akishima, Tokyo, Japan). 2.3. MSC Preparation Human bone marrow MSCs were derived from the whole bone marrow of independent human donors (commercially available from AllCells, Alameda, CA, USA). The cells were culturFull Paper
2021Wang, X;Sandberg, ML;Martin, AD;Negri, KR;Gabrelow, GB;Nampe, DP;Wu, ML;McElvain, ME;Toledo Warshaviak, D;Lee, WH;Oh, J;Daris, ME;Chai, F;Yao, C;Furney, J;Pigott, C;Kamb, A;Xu, H;Potent, Selective CARs as Potential T-Cell Therapeutics for HPV-positive CancersJournal of immunotherapy (Hagerstown, Md. : 1997)34432728For human peripheral blood mononuclear cells (PBMCs), collection protocols and donor informed-consent were approved by an Institutional Review Board at Allcells. Allcells followed SaveFull Paper
2021Martin, AD;Wang, X;Sandberg, ML;Negri, KR;Wu, ML;Toledo Warshaviak, D;Gabrelow, GB;McElvain, ME;Lee, B;Daris, ME;Xu, H;Kamb, A;Re-examination of MAGE-A3 as a T-cell Therapeutic TargetJournal of immunotherapy (Hagerstown, Md. : 1997)33284140purchased from Allcells according to the method described by Garcia et al31 Collection protocols and donor informed-consent were approved by an institutional review board at Allcells. SaveFull Paper
2021Xu, F;Liu, X;Zhang, D;Zhao, F;Fan, Z;Hu, S;Mei, S;Huang, Y;Sun, H;Wei, L;Guo, L;Wang, J;Cen, S;Liang, C;Guo, F;The Engineered MARCH8-Resistant Vesicular Stomatitis Virus Glycoprotein Enhances Lentiviral Vector TransductionHuman gene therapy33678011Peripheral blood mononuclear cells (PBMCs) isolated from healthy donors were purchased from AllCells (Shanghai, China), and cultured in RPMI 1640 media containing 10% FBS ( SaveFull Paper
2021Chen, Z;Chen, Z;Xu, S;Zhang, Q;LncRNA SOX2-OT/miR-30d-5p/PDK1 Regulates PD-L1 Checkpoint Through the mTOR Signaling Pathway to Promote Non-small Cell Lung Cancer Progression and Immune EscapeFrontiers in GeneticsThe microporous 2-compartment co-culture system was applied for co-culture. Transfected NSCLC cells were inoculated in the upper chamber, while CD8+ T (PB009-3-C, ALLCELLS, Shanghai) cells were inoculated in the lower chamber, allowing direct contactFull Paper
2021Raskin, S;Van Pelt, S;Toner, K;Balakrishnan, PB;Dave, H;Bollard, CM;Yvon, E;Novel TCR-like CAR-T cells targeting an HLA∗0201-restricted SSX2 epitope display strong activity against acute myeloid leukemiaMolecular therapy. Methods & clinical development34729377Steady-state apheresis units were purchased from AllCells and peripheral blood mononuclear cells (PBMCs) were isolated using lymphoprep density gradient. Before cryopreserving Save;ng a phase I clinical trial to evaluate the safety and anti-tumor eFull Paper
2021Xue, L;Gao, X;Zhang, H;Tang, J;Wang, Q;Li, F;Li, X;Yu, X;Lu, Z;Huang, Y;Tang, R;Yang, W;Antiangiogenic antibody BD0801 combined with immune checkpoint inhibitors achieves synergistic antitumor activity and affects the tumor microenvironmentBMC cancer34686154#0025), 1% penicillin/streptomycin (GIBCO, Invitrogen Inc., Carlsbad, CA, USA, #15140-122) and 1% ECGS (ScienCell, #1052). For HUVEC Western blot, migration inhibition, and scratch assays, HUVECs (AllCells, Alameda, CA, USA) were maintained in HUVECFull Paper
2021Noaks, E;Peticone, C;Kotsopoulou, E;Bracewell, DG;Enriching leukapheresis improves T cell activation and transduction efficiency during CAR T processingMolecular therapy. Methods & clinical development33718517Peripheral blood leukapheresis products were obtained from the NHS or purchased as LeukoPaks from AllCells, Cellex, and Anthony Nolan as part of research study NREC ID 15/LO/ Save;omplex and expensive magnetic cell sorting. Materials and methods CuFull Paper
2021Green, R;Elliott, JL;Norona, W;Go, F;Nguyen, VT;Ge, J;Short, ML;Mulero, JJ;Zhong, C;Developmental validation of VeriFiler™ Plus PCR Amplification Kit: A 6-dye multiplex assay designed for casework samplesForensic science international. Genetics33740707Human genomic DNA used for the degraded DNA experiments was extracted from normal peripheral blood mononuclear cells (PBMC) of a male donor (ALLCELLS, Emeryville, CA) using a QiAamp DNA Blood Maxi Kit (Qiagen)Full Paper
2021Park, S;Huang, NWY;Wong, CXY;Pan, J;Albakr, L;Gu, J;Kang, L;Microstructured Hyaluronic Acid Hydrogel for Tooth Germ BioengineeringGels (Basel, Switzerland)34449604ovine serum (Thermo Fisher, Waltham, MA, USA), penicillin-Streptomycin solution with 10,000 units penicillin and 10 mg streptomycin / mL (Thermo Fisher, Waltham, MA, USA). The culture media for DPSC (AllCells, Alameda, CA, USA) were α-Minimum essentiFull Paper
2021Garbuzova-Davis, S;Boccio, KJ;Llauget, A;Shell, R;Hailu, S;Mustafa, H;Ehrhart, J;Sanberg, PR;Appel, SH;Borlongan, CV;Beneficial Effects of Transplanted Human Bone Marrow Endothelial Progenitors on Functional and Cellular Components of Blood-Spinal Cord Barrier in ALS MiceeNeuro34479980Cryopreserved human bone marrow CD34+ cells (hBM34+) and human bone marrow-derived endothelial progenitor cells (hBM-EPCs) were purchased from AllCells and CELPROGEN SaveFull Paper
2021Chu, J;Pieles, O;Pfeifer, CG;Alt, V;Morsczeck, C;Docheva, D;Dental follicle cell differentiation towards periodontal ligament-like tissue in a self-assembly three-dimensional organoid modelEuropean cells & materials34251657according to AllCells company regulations, further donor information such gender and exact age was not provided to the purchaser). The PDL-hTERT immortal cell line has already SaveFull Paper
2021Madanagopal, TT;Tai, YK;Lim, SH;Fong, CH;Cao, T;Rosa, V;Franco-Obregón, A;Pulsed electromagnetic fields synergize with graphene to enhance dental pulp stem cell-derived neurogenesis by selectively targeting TRPC1 channelsEuropean cells & materials33644848hDPSC culture The use of human cells was approved by the NUS Institutional Review Board (NUS2094/2015). hDPSC (DPF003, AllCells, Quincy, MA, USA) were cultured in basal growth medium (DMEM, Invitrogen), supplemented with 10 % FBS (Invitrogen) in theFull Paper
2021Wang, X;Martin, AD;Negri, KR;McElvain, ME;Oh, J;Wu, ML;Lee, WH;Ando, Y;Gabrelow, GB;Toledo Warshaviak, D;Sandberg, ML;Xu, H;Kamb, A;Extensive functional comparisons between chimeric antigen receptors and T cell receptors highlight fundamental similaritiesMolecular immunology34419823Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols SaveFull Paper
2021Zacharia, A;Harberts, E;Valencia, SM;Myers, B;Sanders, C;Jain, A;Larson, NR;Middaugh, CR;Picking, WD;Difilippantonio, S;Kirnbauer, R;Roden, RB;Pinto, LA;Shoemaker, RH;Ernst, RK;Marshall, JD;Optimization of RG1-VLP vaccine performance in mice with novel TLR4 agonistsVaccine33309485cells were also cultured in the presence of BECC470 or TLR4 agonists and the resulting upregulation of surface co-stimulatory markers was measured by flow cytometry (AllCells). SaveFull Paper
2021Liu, C;Yang, G;Zhou, M;Zhang, X;Wu, X;Wu, P;Gu, X;Jiang, X;Magnesium Ammonium Phosphate Composite Cell-Laden Hydrogel Promotes Osteogenesis and Angiogenesis In VitroACS omega33869925Human umbilical vein endothelial cells (HUVECs; eahy926) were purchased from AllCells and cultured in EBM. They were seeded in the upper chamber, while the lower chambers SaveFull Paper
2021Jangjoo, M;Goodman, SJ;Choufani, S;Trost, B;Scherer, SW;Kelley, E;Ayub, M;Nicolson, R;Georgiades, S;Crosbie, J;Schachar, R;Anagnostou, E;Grunebaum, E;Weksberg, R;An Epigenetically Distinct Subset of Children With Autism Spectrum Disorder Resulting From Differences in Blood Cell CompositionFrontiers in neurology33935932ed, B cell, CD4T, CD8T, monocyte, granulocytes, and NK. These blood cell types were those on which the Houseman method was originally trained, using peripheral blood leukocyte subtypes purchased from AllCells , LLC (Emeryville, CA) or sorted cells frFull Paper
2021Zhou, N;Chen, Y;Yang, L;Xu, T;Wang, F;Chen, L;Liu, J;Liu, G;LncRNA SNHG4 promotes malignant biological behaviors and immune escape of colorectal cancer cells by regulating the miR-144-3p/MET axisAmerican journal of translational research34786048The transfected CACO2 cells were co-cultured with CD4+ T cells (PB009-2-C, AllCells Biotechnology Co., Ltd., Shanghai, China), followed by the addition of PD-1 antibody (ab52587, Abcam, UK) and PD-L1 antibody (ab205921, Abcam, UK). CD4+ T cell isolatFull Paper
2021Horton, TM;Hoff, FW;van Dijk, A;Jenkins, GN;Morrison, D;Bhatla, T;Hogan, L;Romanos-Sirakis, E;Meyer, J;Carroll, WL;Qiu, Y;Wang, T;Mo, Q;Kornblau, SM;The effects of sample handling on proteomics assessed by reverse phase protein arrays (RPPA): Functional proteomic profiling in leukemiaJournal of proteomics33212251Normalization controls were commercially available normal adult CD34+ bone marrow cells (AllCells). Peripheral blood mononuclear cells (PBMC) were isolated using Lymphoprep SaveFull Paper
2021Khouri, J;Faiman, BM;Grabowski, D;Mahfouz, RZ;Khan, SN;Wei, W;Valent, J;Dean, R;Samaras, C;Jha, BK;Lazarus, H;Campagnaro, EL;Malek, E;Reed, J;Karam, MA;Hamilton, K;Fada, S;Kalaycio, M;Liu, H;Sobecks, R;Saunthararajah, Y;Chew, Y;Orloff, M;Reu, FJ;DNA methylation inhibition in myeloma: Experience from a phase 1b study of low-dose continuous azacitidine in combination with lenalidomide and low-dose dexamethasone in relapsed or refractory multiple myelomaSeminars in hematology33509443Normal bone marrow mononuclear cells for separation of CD138 positive and negative fractions of 3 healthy donors was obtained commercially from ALLCELLS and processed the SaveFull Paper
2021Schölzel, C;Blesius, V;Ernst, G;Goesmann, A;Dominik, A;Countering reproducibility issues in mathematical models with software engineering techniques: A case study using a one-dimensional mathematical model of the atrioventricular nodePloS one34280231This means that the release version of the AllCells model is not only executable in OpenModelica, but also in any of the over 100 tools that support the Functional Mock-up Interface (FMI SaveFull Paper
2021Dulay, RM;Valdez, BC;Li, Y;Chakrabarti, S;Dhillon, B;Kalaw, SP;Reyes, RG;Cabrera, EC;Cytotoxicity of Gymnopilus purpureosquamulosus extracts on hematologic malignant cells through activation of the SAPK/JNK signaling pathwayPloS one34048499esuspended in freezing medium (50% heat-inactivated fetal bovine serum, 40% RPMI, 10% DMSO), and cryopreserved in liquid nitrogen until used. Mononuclear cells from healthy donors were purchased from ALLCELLS LLC (Almeda, CA, USA). For extract exposuFull Paper
2021Caplan, HW;Prabhakara, KS;Toledano Furman, NE;Zorofchian, S;Martin, C;Xue, H;Olson, SD;Cox, CS;Human-derived Treg and MSC combination therapy may augment immunosuppressive potency in vitro, but did not improve blood brain barrier integrity in an experimental rat traumatic brain injury modelPloS one34038436Bone marrow-derived MSC were isolated from a commercially available fresh human bone marrow aspirate (AllCells, Alameda, CA) and expanded following established procedures [8, SaveFull Paper
2021Baik, R;Wyman, SK;Kabir, S;Corn, JE;Genome editing to model and reverse a prevalent mutation associated with myeloproliferative neoplasmsPloS one33661998Frozen human mobilized peripheral blood CD34+ HSPCs were purchased from AllCells and thawed according to manufacturer's instructions. CD34+ cells were cultured in StemSpan SaveFull Paper
2021Garbuzova-Davis, S;Boccio, KJ;Ehrhart, J;Sanberg, PR;Appel, SH;Borlongan, CV;Detection of endothelial cell-associated human DNA reveals transplanted human bone marrow stem cell engraftment into CNS capillaries of ALS miceBrain research bulletin33545308Cryopreserved human bone marrow CD34+ cells (hBM34+) and human bone marrow-derived endothelial progenitor cells (hBM-EPCs) were purchased from AllCells (Alameda, CA, USA) and CELPROGEN (Torrance, CA, USA), respectively. According to the company reporFull Paper
2021Montalban-Bravo, G;Darbaniyan, F;Kanagal-Shamanna, R;Ganan-Gomez, I;Class, CA;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, KA;Chien, KS;Bueso-Ramos, C;Do, KA;Kantarjian, H;Garcia-Manero, G;Type I interferon upregulation and deregulation of genes involved in monopoiesis in chronic myelomonocytic leukemiaLeukemia research33517186BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). Informed consent was obtained according to protocols approved by the MDACC institutional review SaveFull Paper
2021Lu, J;Collins, P;Lee, KJ;Li, CM;Wang, S;In vitro assessment of an engineered tBID-based safety switch system in human T lymphocytesAnnals of translational medicine34988150n 12-well plate and incubated the plate in incubator at 37 °C. Human T-cell samples were purchased anonymously from the vendor and considered exempt from IRB approval. The frozen human pan-T cells (AllCells, LLC., Cat# S-ISO004-06) were thawed and cFull Paper
2021Maucort, C;Di Giorgio, A;Azoulay, S;Duca, M;Differentiation of Cancer Stem Cells by Using Synthetic Small Molecules: Toward New Therapeutic Strategies against Therapy ResistanceChemMedChem32803855In an analogous work, it was found that ICG-001 could safely eradicate the drug-resistant CSC-like population and initiate cell differentiation in leukemia (pre-B ALLcells). The same SaveFull Paper
2021Hu, J;Zhang, J;Yu, M;Liu, Z;Zou, Y;Hong, L;Zhang, T;Sun, J;Zheng, M;Zhu, X;Wang, Z;Liu, S;Circulating miR-221/222 reduces CD4+ T cells by inhibiting CD4 expression in colorectal cancerActa biochimica et biophysica Sinica34357372Peripheral blood mononuclear cells (PBMCs) were purchased from ALLCELLS (PB005F; Shanghai, China). PBMCs were incubated with DynaBeads (11141D; Gibco, Carlsbad, USA), and CD3+ T cells were isolated in a magnet by binding to DynaBeads containing anti-Full Paper
2021Yang, Y;Wang, C;Dai, C;Liu, X;Li, W;Huang, M;Zhao, X;Ji, D;Li, J;Guo, W;Amplification and expression of c-MET correlate with poor prognosis of patients with gastric cancer and upregulate the expression of PDL1Acta biochimica et biophysica Sinica33693450The prognostic significance of c-MET in gastric cancer (GC) remains uncertain. In the present study, we examined the amplification, expression, and the prognostic value of c-MET, SaveFull Paper
2021Thorarensen, A;Balbo, P;Banker, ME;Czerwinski, RM;Kuhn, M;Maurer, TS;Telliez, JB;Vincent, F;Wittwer, AJ;The advantages of describing covalent inhibitor in vitro potencies by IC50 at a fixed time point. IC50 determination of covalent inhibitors provides meaningful data to medicinal chemistry for SAR optimizationBioorganic & medicinal chemistry33285410Cryopreserved human PBMCs (Catalog No. PB005F), which were used in the IL-2, IL-10, IL-15, and IFNα assays, were purchased from Allcells (Emeryville, CA). Frozen PBMCs were thawed in a water bath (37 °C), and washed once with RPMI1640 medium (CatalogFull Paper
2021Jiang, J;Li, X;Mao, F;Wu, X;Chen, Y;Small molecular fluorescence dyes for immuno cell analysisAnalytical biochemistry33306976Statement of Ethical Use of Human Samples: All human PBMCs used in this study were obtained from AllCells Alameda, 1301 Harbor Bay Parkway, Suite 200, Alameda, CA 94502, Save;h as leukemia; and provide a quick and efficient way to study the immuneFull Paper
2021Takahashi, N;Higa, A;Hiyama, G;Tamura, H;Hoshi, H;Dobashi, Y;Katahira, K;Ishihara, H;Takagi, K;Goda, K;Okabe, N;Muto, S;Suzuki, H;Shimomura, K;Watanabe, S;Takagi, M;Construction of in vitro patient-derived tumor models to evaluate anticancer agents and cancer immunotherapyOncology letters33841567patient, were used in this study. Patient-derived hematopoietic tumor cells were provided by Fukushima Medical University Hospital, the Japanese Pediatric Cancer Group ALL-R14 study, ProteoGenex, or AllCells. Peripheral blood mononuclear cells (PBMCsFull Paper
2021Yoshida, K;Takabayashi, T;Imoto, Y;Sakashita, M;Kato, Y;Narita, N;Fujieda, S;Increased Thrombin-Activatable Fibrinolysis Inhibitor in Response to Sublingual Immunotherapy for Allergic RhinitisThe Laryngoscope33844301Systems, Minneapolis, Minnesota ), 100 ng IL-13 (R&D Systems), 100 ng IL-4 plus 1 μg/mL TAFI (Abcam, Cambridge, UK), and 100 ng IL-13 with 1 μg/mL TAFI. Human mast cells were differentiated from CD34+ hematopoietic progenitor cells (ALLCELLS, AlamedFull Paper
2021Hashim, SNM;Yusof, MFH;Zahari, W;Noordin, KBAA;Akamatsu, T;Azlina, A;Amniotic membrane matrix effects on calcineurin-NFAT-related gene expressions of SHED treated with VEGF for endothelial differentiationIn vitro cellular & developmental biology. Animal34021476SHED (AllCells, Alameda, California) were cultured in a complete medium made of the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, Waltham, Massachusetts), supplemented with 15% of fetal bovine serum (FBS) (Gibco, Waltham, Massachusetts)Full Paper
2021Pieles, O;Hartmann, M;Morsczeck, C;AMP-activated protein kinase and the down-stream activated process of autophagy regulate the osteogenic differentiation of human dental follicle cellsArchives of oral biology332540472.1. Cell culture Human dental follicle cells were purchased from AllCells and cultivated in DMEM (Dulbecco’s Modified Eagle’s Medium) high glucose with 10 % FBS (fetal bovine serum) and 1% penicillin (10,000 units/mL) / streptomycin (10 mg/mL) solutFull Paper
2021Wang, Z;Jiang, L;Wang, J;Chai, Z;Xiong, W;Morphine promotes angiogenesis by activating PI3K/Akt/HIF-1α pathway and upregulating VEGF in hepatocellular carcinomaJournal of gastrointestinal oncology34532126Academy of Science (Shanghai, China). The cells were cultured in 10% FBS DMEM, 37 °C, 5% CO2 incubator. HUVECs were obtained from the Allcells Company(Shanghai, China) and cultured in 10% FBS Endothelial Cell Growth Medium (ECM, Lonza, Switzerland),Full Paper
2021Hayashi, Y;Kawaki, H;Hori, M;Shintani, K;Hasegawa, T;Tanaka, M;Kondoh, N;Yoshida, T;Kawano, S;Tamaki, Y;Evaluation of the mechanical properties and biocompatibility of gypsum-containing calcium silicate cementsDental materials journal33642445Evaluation of the cytotoxicity of cement-immersed media Samples of hDPSCs (Allcells, Emeryville, CA, USA), which had been subcultured 11-14 times in 10% fetal bovine serum (FBS)-containing α-MEM, were seeded within a 96-well plate at a density of 4,0Full Paper
2021Iwasa, M;Fujii, S;Fujishiro, A;Maekawa, T;Andoh, A;Takaori-Kondo, A;Ichinohe, T;Miura, Y;Impact of 2 Gy γ-irradiation on the hallmark characteristics of human bone marrow-derived MSCsInternational journal of hematology33386593Bone marrow of multiple healthy adults aged 21-44 years (#4362, #4615, and #4641) was purchased from AllCells (Emeryville, CA). Human BM-MSCs were isolated from bone marrow according to our previously published methods [12, 13]. In brief, a single-ceFull Paper
2021Cui, L;Yin, F;Cheng, J;Liu, H;Zheng, M;Liu, D;Wu, Z;Qian, Q;Optimized cytotoxicity assay for co-suspended effector and target cellsJournal of immunological methods34270976Peripheral blood mononuclear cells (PBMCs) obtained from whole blood of healthy volunteers (AllCells, Silicon Valley, CA, USA) were isolated by density gradient centrifugation with SaveFull Paper
2021Sunderic, K;Li, C;Ahmed, AHR;Dawkins, D;Azar, T;Cardoso, L;Wang, S;Tuning Thermal Dosage to Facilitate Mesenchymal Stem Cell Osteogenesis in Pro-Inflammatory EnvironmentJournal of biomechanical engineering32601701Human bone marrow explanted from the iliac crest of donors was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the RosetteSep SaveFull Paper
2021Koh, H;Kwon, SY;Zhen, X;Ha, HY;Lee, JH;Generation of induced pluripotent stem cell line (KRIBBi004-A) from adult bone marrow CD34+ cells from a patient carrying 46,XX,t(1;5)(p31.1;35.1) karyotypeStem cell research34736040Cell Source iPSC was derived from CD34+ bone marrow (BM) cells, obtained from AllCells, LLC Clonality Clonal Associated disease N/A Gene/locus Chromosome translocation t(1;5)(p31.1;35.1) Date archived/stock date Jun.2021 Cell line repository/bank TheFull Paper
2021Kim, J;Koh, H;Zhen, X;Lee, DS;Ha, HY;Lee, JH;Establishment of iPSC (KRIBBi001-A) from CD34+ group O D-negative bone marrow bloodStem cell research33529979Cell Source iPSC was derived from CD34+ bone marrow (BM) cells, obtained from AllCells, LLC Clonality Clonal Method of reprogramming Integration-free Sendai virus-based vectors (CytoTune™-iPS 2.0 Sendai Reprogramming Kit, Life Technologies)Full Paper
2021Fu, X;Shen, X;Yin, X;Zhang, Y;Wang, X;Han, Z;Lin, Q;Fan, J;Antioxidant and pro-apoptosis activities of coffee husk (Coffea arabica) anthocyaninsInternational Food Research JournalThe HUVEC and Caco-2 cell lines were purchased from ALLCELLS Biotechnology Company (Shanghai, China) and Kunming Institute of Zoology Cell Line Bank (Yunnan, China), respectively. Dulbecco’s Modified Eagle’s Medium (DMEM), foetal bovine serum, peniciFull Paper
2021Choi, J;Goulding, SP;Conn, BP;McGann, CD;Dietze, JL;Kohler, J;Lenkala, D;Boudot, A;Rothenberg, DA;Turcott, PJ;Srouji, JR;Foley, KC;Rooney, MS;van Buuren, MM;Gaynor, RB;Abelin, JG;Addona, TA;Juneja, VR;Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutationsCell reports methods35474673(HLA-A∗03:01, HLA-A∗11:01, HLA-A∗68:01, HLA-B∗07:02, HLA-C∗01:02, HLA-C∗03:04, and/or HLA-C∗08:02) were isolated using Ficoll separation from apheresis material (AllCells Save;130-050-201; RRID:AB_2665482 CD25 MicroBeads, human Miltenyi BiotecFull Paper
2021Cai, W;Dong, J;Gallolu Kankanamalage, S;Titong, A;Shi, J;Jia, Z;Wang, B;Huang, C;Zhang, J;Lin, J;Kan, SZ;Han, S;Zhou, J;Liu, Y;Biological activity validation of a computationally designed Rituximab/CD3 T cell engager targeting CD20+ cancers with multiple mechanisms of actionAntibody therapeutics34805746They were cultured in RPMI 1640 (Thermo fisher) medium supplemented with 10% fetal bovine serum (FBS) (GIBCO) at 37 °C, 5% CO2. Human peripheral blood mononuclear cells (hPBMC) were purchased from AllCells (Alameda, CA) and HumanCells Bio (San Jose,Full Paper
2021Moe, GR;Steirer, LM;Lee, JA;Shivakumar, A;Bolanos, AD;A cancer-unique glycan: de-N-acetyl polysialic acid (dPSA) linked to cell surface nucleolin depends on re-expression of the fetal polysialyltransferase ST8SIA2 geneJournal of experimental & clinical cancer research : CR34544457rting (FACS) binding, and fluorescence labeling experiments were treated with Accutase and washed with medium before use. Human normal peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Alameda, CA). Protein extraction from ceFull Paper
2021Xing, Y;Chu, KA;Wadhwa, J;Chen, W;Zhu, J;Bradshaw, JM;Shu, J;Foulke, MC;Loewenstein, N;Nunn, P;By, K;Phiasivongsa, P;Goldstein, DM;Langrish, CL;Preclinical Mechanisms of Topical PRN473, a Bruton Tyrosine Kinase Inhibitor, in Immune-Mediated Skin Disease ModelsImmunoHorizons34326199Human whole blood (AllCells) pretreated for 1 h with eleven 3-fold serial dilutions of PRN473, starting at a concentration of 10 μM, was incubated overnight with goat anti-human IgM F( SaveFull Paper
2021Pohlmeyer, CW;Shang, C;Han, P;Cui, ZH;Jones, RM;Clarke, AS;Murray, BP;Lopez, DA;Newstrom, DW;Inzunza, MD;Matzkies, FG;Currie, KS;Di Paolo, JA;Characterization of the mechanism of action of lanraplenib, a novel spleen tyrosine kinase inhibitor, in models of lupus nephritisBMC rheumatology33781343healthy donors or individuals with SLE (AllCells) were treated with lanraplenib or dimethyl Naive B cells were isolated from freshly collected PBMCs (AllCells) by negative selection ( SaveFull Paper
2021Nordin, NS;Mokhtar, KI;Mustafa, BE;Effects of Flaxseed (Linum usitatissimum) Extract on the Osteoblast Differentiation Potential of Stem Cells Derived from Human Exfoliated Deciduous TeethMakara Journal of Health ResearchSHED (ALLCells, USA) were cultured in complete growth media (alpha-MEM; 10% FBS; 0.5% Pen-Strep) until confluence. SHED at 80%-90% confluence were subjected to osteoblast differentiation. The complete growth medium was replaced with osteoblast inductFull Paper
2021Turkson, A;Addor, J;Kharib, D;Validating Intrinsic Factors Informing E-Commerce: Categorical Data Analysis DemoOpen Journal of Statisticsows a chi-square distribution, and the conclusion depends on whether or not the obtained statistic is greater that the critical statistic at a chosen alpha level. The test statistics is: χ 2 = ∑ allcells ( Observed − Expected ) 2 Expected = ∑ i , jFull Paper
2021Xu, Y;Shen, L;Li, G;The The Effect of Elution Volume for Immunoprecipitation on m6A-Seq AnalysisProceedings of Anticancer ResearchThe Mouse Renal Carcinoma cells (RenCa) were cultured in DMEM medium (Invitrogen) containing 10% FBS, 2 mM L-Glutamine and 1% penicillin-streptomycin. RenCa cell line was purchased from Allcells Biotechnology (Shanghai, China) and were routinely testFull Paper
2021Zhou, Z;Zheng, J;Lin, D;Chen, Y;Hu, X;Exosomes Derived from Dental Pulp Stem Cells Accelerate Cutaneous wound Healing by Enhancing Angiogenesis via Cdc42/p38 MAPK PathwayResearch Square1% penicillin-streptomycin (Sigma, USA). HUVECs were bought from ALLCELLS (Shanghai, China) and expanded in endothelial cell growth medium (EGM). For exosome co-cultures experiment, 10μg/ml of exosomes were added to the culture medium of HUVECs. AllFull Paper
2021Blitzer, G;Paz, C;Glassey, A;Ganz, O;Giri, J;Pennati, A;Meyers, R;Lunga, T;Robbins, D;Thibeault, S;Bates, A;Nickel, K;Morris, Z;Mattison, R;Chappell, R;Glazer, T;Rogus-Pulia, N;Galipeau, J;Kimple, R;A Pilot Study to Assess the Salivary Gland Regenerative Potential of Bone Marrow Mesenchymal Stromal Cells from Treated Head and Neck Cancer PatientsResearch SquareBone marrow from healthy adult control volunteers were purchased from a commercial source (AllCells, Alameda, CA). We had originally planned to enroll 15 patients: 5 patients with SaveFull Paper
2021Tie, B;Guo, Z;Li, L;Wang, W;Liu, R;Fang, J;miR-3156-5p Enhances Hypoxia-Induced Angiogenesis in Hepatocellular Carcinoma via Modulating the SOCS5/HIF-1α/VEGF PathwayResearch SquareHuman umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in the medium containing 50 U/mL penicillin and 50 U/mL streptomycin (Invitgen, San Diego, CA), 50 mL endothelial cell growth additive (UpstatFull Paper
2021Chen, S;Barrientos, J;Ferrer, G;Ravichandran, P;Ibrahim, M;Kieso, Y;Kutok, J;Peluso, M;Sharma, S;Weaver, D;Pachter, J;Rai, K;Chiorazzi, N;Duvelisib Eliminates CLL B Cells, Impairs CLL-Supporting Cells, and Overcomes Ibrutinib Resistance in Preclinical ModelsResearch SquareHuman PBMCs from CLL donors obtained from whole blood from a commercial source (Bioreclamation IVT or ALLCELLS) were resuspended (1 x 106 cells/mL) in RPMI-1640 medium SaveFull Paper
2021Valadkhan, S;Plasek, L;Gunawardane, L;Niazi, F;Mason, S;Leskov, K;Perez, G;Dobrowolski, C;Shukla, M;Nutt, W;Karn, J;An HIV-induced mechanism for T cell quiescence andproviral latencyResearch SquarePeripheral blood mononuclear cells from HIV negative Caucasian male donors were purchased from Allcells. Naive CD4+ T cells were isolated using EasySep Human Naive CD4+ T SaveFull Paper
2021Cai, W;Dong, J;Kankanamalage, S;Titong, A;Shi, J;Jia, Z;Wang, B;Huang, C;Zhang, J;Lin, J;Kan, S;Zhou, J;Liu, Y;A computationally designed Rituximab/CD3 T cell engager targeting CD20+ cancers with multiple mechanisms of actionResearch Squaremedium supplemented with 10% fetal bovine serum (FBS) (GIBCO) 115 at 37°C, 5% CO2. Human peripheral blood mononuclear cells (hPBMC) were purchased from 116 AllCells (Alameda, CA) and HumanCells Bio (San Jose, CA). Rituximab resistant Raji cells 117 (Full Paper
2021Zhao, C;Zhang, W;Gong, G;Xie, L;Wang, M;Hu, Y;A New Approach to Produce IgG4-like Bispecific AntibodiesResearch SquareHuman CD4+ T cells were purified from PBMC (AllCells, China) by negative selection with CD4+ T cell enrichment cocktail kit (Stemcell, Vancouver, Canada) according to the manufacturer’s instruction. Immature DC (iDC) was generated from monocytes by cFull Paper
2021Noto, P;Analysis of Liver X Receptor target gene expression across speciesDrexel UniversitycDNA from Human MTC™ Panels I and II were purchased from Clontech (catalog# 636742 and 636743) and subjected to real-time PCR. Whole blood from human donors was purchased from AllCells (catalog# WB001)Full Paper
2021Dunbar, A;Kim, D;Lu, M;Farina, M;Yang, J;Park, Y;Gobbo, F;Verachi, P;Martelli, F;Karzai, A;Xiao, W;Xia, L;Elmansy, N;Kleppe, M;Chen, Z;Xiao, Y;McGovern, E;Snyder, J;Krishnan, A;Hill, C;Cordner, K;Zouak, A;Salama, M;Yohai, J;Tucker, E;Chen, J;Zhou, J;McConnell, T;Koche, R;Rampal, R;Migliaccio, A;Fan, R;Levine, R;Hoffman, R;CXCL8/CXCR2 signaling mediates bone marrow fibrosis and represents a therapeutic target in myelofibrosisbioRxivPro-inflammatory signaling is a hallmark feature of human cancer, including in myeloproliferative neoplasms (MPNs), most notably myelofibrosis (MF). Dysregulated inflammatory SaveFull Paper
2021Kartha, V;Duarte, F;Hu, Y;Ma, S;Chew, J;Lareau, C;Earl, A;Burkett, Z;Kohlway, A;Lebofsky, R;Buenrostro, J;Functional Inference of Gene Regulation using Single-Cell Multi-OmicsbioRxivCD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from AllCells (see Table S1 for catalog numbers and donor information). Cells were quickly thawed in a 37°C water bath, rinsed with culture medium (Iscove’s ModifiedFull Paper
2021Kong, T;Laranjeira, A;Yang, K;Fisher, D;Yu, L;Wang, A;Ruzinova, M;Fowles, J;Allen, M;Celik, H;Challen, G;Huang, S;Oh, S;DUSP6 mediates resistance to JAK2 inhibition and drives leukemic progressionbioRxivgradient extraction and cryopreserved according to standard procedures. Additional BMMCs were purchased from AllCells (Alameda, California). A list of patient samples utilized in this study is provided in Table 1Full Paper
2021Qi, J;D’Souza, D;Dawson, T;Geanon, D;Stefanos, H;Marvin, R;Walker, L;Rahman, A;Multimodal Single-Cell Characterization of the Human Granulocyte LineagebioRxivFresh whole bone marrow was purchased from AllCells Inc. and delivered on the day of the experiment. The bone marrow was in a volume of 3mL and the sample was limited to 2 units/ SaveFull Paper
2021Kim, J;Schulte, A;Sarver, A;Angelos, M;Frantz, A;Forster, C;O’Brien, T;Cornax, I;O’Sullivan, M;Cheng, N;Lewellen, M;Oseth, L;Kumar, S;Bullman, S;Pedamallu, C;Goyal, S;Meyerson, M;Lund, T;Alfoldi, J;Breen, M;Lindblad-Toh, K;Dickerson, E;Kaufman, D;Modiano, J;Transcriptional and Functional Activity of Hemangiosarcoma Support Bone Marrow Nurse Cell OntogenybioRxivHuman BM-derived mesenchymal stromal cells (hBM-MSCs) were isolated from whole bone marrow purchased from AllCells (Emeryville, CA, USA) as previously described [24-27]. M2- SaveFull Paper
2021Maughon, T;Shen, X;Huang, D;Michael, A;Shockey, W;Andrews, S;McRae, J;Platt, M;Fernández, F;Edison, A;Stice, S;Marklein, R;Multi-omics characterization of mesenchymal stem/stromal cells for the identification of putative critical quality attributesbioRxivPBMCs (AllCells, Alameda CA) were thawed in RPMI media (RPMI, 20% FBS, 2mM L-glutamine, 50 U/mL penicillin, 50 μg/mL streptomycin) and cultured overnight at 37°C and 5% CO2. Prior to co-culture, PBMCs were labeled with CFSE (Supplemental Table 1, BioFull Paper
2021Jain, S;Rick, J;Joshi, R;Beniwal, A;Spatz, J;Chang, A;Nguyen, A;Sudhir, S;Chandra, A;Haddad, A;Wadhwa, H;Shah, S;Choi, S;Hayes, J;Wang, L;Yagnik, G;Costello, J;Diaz, A;Aghi, M;Identification of Cancer-Associated Fibroblasts in Glioblastoma and Defining Their Pro-tumoral EffectsbioRxivTHP-1 cells and monocytes isolated from peripheral blood (AllCells) run through the MojoSort Human CD14 Selection Kit were treated with 50 ng/μL PMA (phorbol myristate acetate) SaveFull Paper
2021Andrews, S;Klinker, M;Bauer, S;Marklein, R;Morphological Landscapes from High Content Imaging Identify Optimal Priming Strategies that Enhance MSC ImmunosuppressionbioRxivHuman bone marrow-derived MSCs were obtained from 4 different donors purchased from Lonza (Walkersville, MD, USA), AllCells (Emeryville, CA, USA), and RoosterBio (Frederick, MD, USA) (see Table S1 for donor information). MSC culture conditions for thFull Paper
2021Gu, C;Cao, X;Wang, Z;Hu, X;Yao, Y;Zhou, Y;Liu, P;Liu, X;Gao, G;Hu, X;Zhang, Y;Chen, Z;Gao, L;Peng, Y;Jia, F;Shan, C;Yu, L;Liu, K;Li, N;Guo, W;Jiang, G;Min, J;Zhang, J;Yang, L;Shi, M;Hou, T;Li, Y;Liang, W;Lu, G;Yang, C;Wang, Y;Xia, K;Xiao, Z;Xue, J;Huang, X;Chen, X;Ma, H;Song, D;Pan, Z;Wang, X;Guo, H;Liang, H;Yuan, Z;Guan, W;Deng, S;A human antibody with blocking activity to RBD proteins of multiple SARS-CoV-2 variants including B.1.351 showed potent prophylactic and therapeutic efficacy against SARS-CoV-2 in rhesus macaquesbioRxivPhage libraries were constructed using antibody gene fragments amplified from PBMCs of 50 healthy human subjects (Allcells, PB003F and PB003C) and total RNA from PBMCs ( SaveFull Paper
2021Fitzgerald, A;Marcisak, E;Nasir, A;Glasgow, E;Jablonski, S;Van Der Veken, P;Pearson, G;Fertig, E;Mace, E;Weiner, L;Fibroblast activation protein regulates natural killer cell migration, extravasation and tumor infiltrationbioRxivfrom AllCells with either CD56 positive selection or CD56 negative selection (Allcells, cat# T cells, B cells and monocytes were isolated from PBMCs (Allcells) using Mojosort magnetic SaveFull Paper
2021Brooks, J;Preclinical Dynamic Contrast Enhanced Imaging for Longitudinal Biophysical Assessments of the Healthy and Malignant Vasculature after RadiotherapyThesisC57BL/6J mice (Strain 000664, Jackson Laboratory, Bar Harbor, ME) mice were injected with 1x106 GFP+ BCR-ABL (p190Kd) expressing B-cell ALLcells [66], suspended in 200 µl of phosphate buffered saline, through the tail vein. To test drug delivery, injFull Paper
2021O'Connor, EI;Mechanisms Behind the Causal Role of Dietary Oxylipins of the Lipoxygenase Pathway in the Development of Pulmonary Arterial HypertensionThesisPAECs were incubated with or without 15-HETE and 12 hours later healthy CD8 cells (ALLCELLS, Lot# 3003028) were added. After 12 hours of contact between EC and CD8 cells, the cells were collected, fixed, and stained for cleaved caspase 3Full Paper
2021McIntyre, P;The Effects of a Pyk2 Kinase Inhibitor on the Proliferation and Differentiation of Human Dental Pulp Stem CellsThesisCommercially available human DPSCs, collected from the pulp of extracted teeth, have been previously purchased from ALLCells and stored in liquid nitrogen (Alameda, CA, USA). • SaveFull Paper
2021Noaks, E;Enhancing the consistency of donor derived peripheral blood mononuclear cells for CAR T-cell manufacturingThesisFor work completed at the company site healthy peripheral blood leukapheresis were obtained from the NHS or purchased as LeukoPaks from AllCells, Cellex and Anthony Nolan, as part of research study NREC ID 15/LO/1322. Experiments using healthy leukapFull Paper
2021Osman, ZF;Zahari, W;Kannan, TP;Ultrastructure changes in BMP-2-treated SHED cultured on glycerol preserved human amniotic membraneActa MicroscópicaommerciallysourcedSHED(AllCells,USA)wasculturedonde-epithelialized2cmx2cmglycerol-preserved γ-radiated AM in a culture dish containing 3 mlof media at different cell density for the respective days asthefollowing;2x105forday1,5x104forday7,2.5x104Full Paper
2021Luecken, MD;Burkhardt, DB;Cannoodt, R;A sandbox for prediction and integration of dna, rna, and proteins in single cellsConferenceWe sourced multiple samples of BMMCs from 10 donors via AllCells (California, USA), all healthy non-smokers without recent medical treatment. Donors varied by age (22 - 40), sex, SaveFull Paper
2021Vladimirova, T;Implementation and Evaluation of Verlet List-based Methods in AutoPasThesisInside the 2D vector for AllCells or 3D vector for CellPair respectively, the method adds the particle indices corresponding to the particle pointers from the same spot in the AoS neighbor SaveFull Paper
2021Maser, IP;In-depth characterization of myeloid enhanced humanized mouse strainsThesiswas diluted with 0.9% sodium chloride (Braun) and 15 mg/kg was intraperitoneally (i.p.) injected into mice. 24 hours later 1×105 human CD34+ umbilical cord blood-derived hematopoietic stem cells (HSCs) (Stem Cell Technologies/Allcells) in 100 µl phosFull Paper
2021Fitzgerald, AA;Enhancing Anti-Cancer Properties of Human Natural Killer CellsThesisFresh healthy donor NK cells were purchased from AllCells with either CD56 positive selection or CD56 negative selection (Allcells, cat#PB012-P or PB012-N). T cells, B cells and monocytes were isolated from PBMCs (Allcells) using Mojosort magnetic ceFull Paper
2021Butler, A;Computational Methods for the Integration of Single-Cell DataThesisWe therefore repeated the original experiment, where PBMCs from a healthy human donor (ALLCELLS) were cultured with RPMI medium supplemented with 10% FBS and stimulated for 6 h with IFNβ (100 U/ml, PBL Assay Science), with a subset of cells left unexFull Paper
2021Nataraja, S;Singh, M;Demes, S;Olson, L;Stanwix, J;Biddle, M;Clarke, E;Huang, Y;Nguyen, J;Chen, C;Zhang, P;Abdulla, F;Vercellotti, G;Belcher, J;ML-0207/ASP8731: A Novel BACH1 Inhibitor That Induces Fetal Hemoglobin in Treatment of Sickle Cell DiseaseBloodTranslational Development, Mitobridge, Cambridge, MA++Translational Science, Mitobridge, Cambridge, MA++Clinical Pharmacology and Exploratory Development, Astellas, Northbrook, IL++Translational Science, Mitobridge, Cambridge, MA++Translational ScienFull Paper
2021Qing, Y;Dong, L;Gao, L;Li, C;Li, Y;Han, L;Prince, E;Tan, B;Deng, X;Wetzel, C;Shen, C;Gao, M;Chen, Z;Li, W;Zhang, B;Braas, D;Ten Hoeve, J;Sanchez, GJ;Chen, H;Chan, LN;Chen, CW;Ann, D;Jiang, L;Müschen, M;Marcucci, G;Plas, DR;Li, Z;Su, R;Chen, J;R-2-hydroxyglutarate attenuates aerobic glycolysis in leukemia by targeting the FTO/m6A/PFKP/LDHB axisMolecular cell33434505The Lin− progenitor cells were then subject to retroviral transduction with MSCV-Neo-MA9 construct by two rounds of ‘spinoculation’, as described previously (Li et al., 2015). After being selected for 7 days with 0.5 mg/mL G418 Sulfate (10131027, GibFull Paper
2021Li, C;Wang, H;Georgakopoulou, A;Gil, S;Yannaki, E;Lieber, A;In Vivo HSC Gene Therapy Using a Bi-modular HDAd5/35++ Vector Cures Sickle Cell Disease in a Mouse ModelMolecular therapy : the journal of the American Society of Gene Therapy32949495The lineage-minus (Lin−) cells were isolated by depletion of lineage-committed cells in bone marrow MNCs using the Mouse Lineage Cell Depletion Kit (Miltenyi Biotec, San Diego, CA, USA) according to the manufacturer’s instructions. CFU assays were peFull Paper
2021Loftus, JP;Yahiaoui, A;Brown, PA;Niswander, LM;Bagashev, A;Wang, M;Schauf, A;Tannheimer, S;Tasian, SK;Combinatorial efficacy of entospletinib and chemotherapy in patient-derived xenograft models of infant acute lymphoblastic leukemiaHaematologica32414848s Supplementary Appendix Acknowledgments We acknowledge Dr Ann Forslund and Ms Chelsea Mullins formerly at Gilead Sciences for study protocol management and data analysis, and Dr Emer Clarke at ReachBio Research Labs for assistance with experimFull Paper
2021DiLillo, DJ;Olson, K;Mohrs, K;Meagher, TC;Bray, K;Sineshchekova, O;Startz, T;Kuhnert, J;Retter, MW;Godin, S;Sharma, P;Delfino, F;Lin, J;Smith, E;Thurston, G;Kirshner, JR;A BCMAxCD3 bispecific T cell-engaging antibody demonstrates robust antitumor efficacy similar to that of anti-BCMA CAR T cellsBlood advances33651100T cells were purified from normal donor PBMCs (ReachBio) by using magnetic beads (Thermo Fisher Scientific) and activated with CD3/CD28 microbeads (Thermo Fisher Scientific) in SaveFull Paper
2021Byrgazov, K;Besse, A;Kraus, M;Slipicevic, A;Lehmann, F;Driessen, C;Besse, L;Novel Peptide-drug Conjugate Melflufen Efficiently Eradicates Bortezomib-resistant Multiple Myeloma Cells Including Tumor-initiating Myeloma Progenitor CellsHemaSphere34136753of MM were assessed in a semisolid methylcellulose-based media formulation containing 10% phytohemagglutinin (PHA)-stimulated leukocyte conditioned medium (ReachBio, WA). Save;cell outgrowth assay Clonogenic progenitors of MM were assessed in a semFull Paper
2021Swaminathan, M;Kopyt, N;Atta, MG;Radhakrishnan, J;Umanath, K;Nguyen, S;O'Rourke, B;Allen, A;Vaninov, N;Tilles, A;LaPointe, E;Blair, A;Gemmiti, C;Miller, B;Parekkadan, B;Barcia, RN;Pharmacological effects of ex vivo mesenchymal stem cell immunotherapy in patients with acute kidney injury and underlying systemic inflammationStem cells translational medicine34581517Flow cytometry testing of patient leukocytes was performed by ReachBio (Spokane, Washington). Blood was collected in CPT tubes (362 753; BD Biosciences), and peripheral blood Save;n a plasma preparation tube (362 788; BD Biosciences, San Jose, CaliFull Paper
2021Lin, M;Kowolik, CM;Xie, J;Yadav, S;Overman, LE;Horne, DA;Potent Anticancer Effects of Epidithiodiketopiperazine NT1721 in Cutaneous T Cell LymphomaCancers34282785TCC (Manassas, VA, USA), authenticated by STR-profiling at the source and passaged for less than 6 months after receipt or resuscitation. Peripheral blood mononuclear cells (PBMCs) were obtained from ReachBio (Spokane, WA, USA). All cells were grownFull Paper
2021Samuelson, C;Radtke, S;Zhu, H;Llewellyn, M;Fields, E;Cook, S;Huang, MW;Jerome, KR;Kiem, HP;Humbert, O;Multiplex CRISPR/Cas9 genome editing in hematopoietic stem cells for fetal hemoglobin reinduction generates chromosomal translocationsMolecular therapy. Methods & clinical development34853798cation event frequencies was also undertaken on each of these tissues from necropsy as per protocol above. CFU and HbF assays on mouse BM A total of 7 × 104/mL cells were plated in ColonyGEL 1402 (ReachBio, Seattle, WA) in triplicate from each mouseFull Paper
2021Sharifi, S;Sharifi, H;Akbari, A;Chodosh, J;Systematic optimization of visible light-induced crosslinking conditions of gelatin methacryloyl (GelMA)Scientific reports34857867and exposed to photoirradiation for a 4 and 10 min. Those cell-laden gels were immediately washed 3 times with PBS and incubated in 200 μL of an optimized serum-containing medium (catalog # 02,100, Reachbio lab, Spokane, WA) at 37 °C. Cell viabilityFull Paper
2021Haber, L;Olson, K;Kelly, MP;Crawford, A;DiLillo, DJ;Tavaré, R;Ullman, E;Mao, S;Canova, L;Sineshchekova, O;Finney, J;Pawashe, A;Patel, S;McKay, R;Rizvi, S;Damko, E;Chiu, D;Vazzana, K;Ram, P;Mohrs, K;D'Orvilliers, A;Xiao, J;Makonnen, S;Hickey, C;Arnold, C;Giurleo, J;Chen, YP;Thwaites, C;Dudgeon, D;Bray, K;Rafique, A;Huang, T;Delfino, F;Hermann, A;Kirshner, JR;Retter, MW;Babb, R;MacDonald, D;Chen, G;Olson, WC;Thurston, G;Davis, S;Lin, JC;Smith, E;Generation of T-cell-redirecting bispecific antibodies with differentiated profiles of cytokine release and biodistribution by CD3 affinity tuningScientific reports34257348ce with the ARRIVE guidelines. OVCAR-3 bioluminescence imaging (BLI) study Eight-week-old NOD SCID gamma chain knock-out (NSG) mice (Jackson Laboratory) were injected with 5 × 106 human PBMCs (ReachBio) before 1 × 106 ascites cells from the OVCAR-3/Full Paper
2021Patterson, AM;Sellamuthu, R;Plett, PA;Sampson, CH;Chua, HL;Fisher, A;Vemula, S;Feng, H;Katz, BP;Tudor, G;Miller, SJ;MacVittie, TJ;Booth, C;Orschell, CM;Establishing Pediatric Mouse Models of the Hematopoietic Acute Radiation Syndrome and the Delayed Effects of Acute Radiation ExposureRadiation research33577641elsewhere (47). Hematopoietic progenitor cell (HPC) number and function were quantitated from 1.0 × 10e5 low-density bone marrow (LDBM) in 1 ml ColonyGEL™ Mouse Complete Medium (ReachBio Research Labs, Seattle, WA)Full Paper
2021Rethnam, M;Tan, DQ;Suda, T;Myeloma cells self-promote migration by regulating TAB1-driven TIMP-1 expression in mesenchymal stem cellsBiochemical and biophysical research communications33183761Human BM MSCs (#02100, ReachBio) were grown in DMEM low glucose media (Biowest) supplemented with 10% FBS and 1% Antibiotic/Anti-mycotic (Gibco). Cells were passaged upon confluence and cultured at a density of 4.5 × 103 cells/cm2. U266-B1 (TIB-196,Full Paper
2021Andersen, A;Vieira-Brock, PL;Vaughan, B;Vollmer, D;Method development for the analysis of PBMC-mediated killing of K562 cells by bovine colostrumJournal of immunological methods34744024Declaration of Competing Interest Authors and principle investigators are employed by 4Life Research, which sells the test articles commercially. 4Life Research funded all portions of this study. Acknowledgements All testing was performed at ReachBiFull Paper
2021Radtke, S;Enstrom, M;Pande, D;Cui, M;Madhu, R;Perez, A;Kiem, H;Hematopoietic recovery after transplantation is primarily derived from the stochastic contribution of hematopoietic stem cellsbioRxivFor CFC assays, 800-1,200 sort-purified CD34+ cells and CD34-subpopulations were seeded into 3.5 ml ColonyGEL 1402 (ReachBio, Seattle, WA). Colonies were scored after 12 to 14 days, discriminating colony forming unit- (CFU-) granulocyte (CFU-G), macrFull Paper
2020Geoerger, B;Kang, HJ;Yalon-Oren, M;Marshall, LV;Vezina, C;Pappo, A;Laetsch, TW;Petrilli, AS;Ebinger, M;Toporski, J;Glade-Bender, J;Nicholls, W;Fox, E;DuBois, SG;Macy, ME;Cohn, SL;Pathiraja, K;Diede, SJ;Ebbinghaus, S;Pinto, N;Pembrolizumab in paediatric patients with advanced melanoma or a PD-L1-positive, advanced, relapsed, or refractory solid tumour or lymphoma (KEYNOTE-051): interim analysis of an open-label, single-arm, phase 1–2 trialLancet Oncol.31812554In all patients, baseline PD-L1 expression in tumour samples (positivity defined as any staining of the stroma or PD-L1 expression on at least 1% of tumour cells) was assessed centrally at QualTek Molecular Laboratories (Goleta, CA, USA) using a protFull Paper
2020Emens, LA;Esteva, FJ;Beresford, M;Saura, C;De Laurentiis, M;Kim, SB;Im, SA;Wang, Y;Salgado, R;Mani, A;Shah, J;Lambertini, C;Liu, H;de Haas, SL;Patre, M;Loi, S;Trastuzumab emtansine plus atezolizumab versus trastuzumab emtansine plus placebo in previously treated, HER2-positive advanced breast cancer (KATE2): a phase 2, multicentre, randomised, double-blind trialThe Lancet. Oncology33002436The KATE2 study is a randomised, double-blind, placebo-controlled, phase 2 trial at 68 centres from nine countries across Asia, Australia, North America, and western Europe (appendix pp 13–15). Eligible patients were adults (aged ≥18 years) with centFull Paper
2020Powell, SF;Gold, KA;Gitau, MM;Sumey, CJ;Lohr, MM;McGraw, SC;Nowak, RK;Jensen, AW;Blanchard, MJ;Fischer, CD;Bykowski, J;Ellison, CA;Black, LJ;Thompson, PA;Callejas-Valera, JL;Lee, JH;Cohen, EEW;Spanos, WC;Safety and Efficacy of Pembrolizumab With Chemoradiotherapy in Locally Advanced Head and Neck Squamous Cell Carcinoma: A Phase IB StudyJ. Clin. Oncol.32479189Testing was performed centrally by QualTek Laboratories (Santa Barbara, CA) using the PD-L1 IHC 22C3 pharmDx (Dako North America, Carpinteria, CA) IHC assay as previously described. 25 PD-L1 evaluation was performed using the combined proportional scFull Paper
2020Marabelle, A;Cassier, P;Fakih, M;Kao, S;Nielsen, D;Italiano, A;Guren, T;Dongen, M;Spencer, K;Bariani, G;Ascierto, P;Santoro, A;Hiret, S;Ott, P;Piha-Paul, S;Liu, C;Leiby, M;Norwood, K;Delord, J;Pembrolizumab for previously treated advanced anal squamous cell carcinoma: Pooled results from the KEYNOTE-028 and KEYNOTE-158 studies.JCOPD-L1 positivity). Baseline PD-L1 expression was assessed using a prototype IHC assay (QualTek) in KEYNOTE-028 and the PD-L1 IHC 22C3 pharmDx assay (Agilent Technologies) in KEYNOTE-158. Pts received pembroFull Paper
2020Greenstein, A;Munster, P;Sachdev, J;Fleming, G;Grauer, A;Shepherd, S;Impact of relacorilant, a selective glucocorticoid receptor antagonist, on the immunosuppressive effects of endogenous cortisol.JCOTina K. Schlafly of Corcept Therapeutics. Funding for editorial, design, and production support for this poster was provided by Corcept to MedVal Scientific Information Services, LLC, Princeton, NJ. Experiments were conducted at Ardigen SA, QualTek MFull Paper
2020Dudek, AZ;Liu, LC;Gupta, S;Logan, TF;Singer, EA;Joshi, M;Zakharia, YN;Lang, JM;Schwarz, JK;Al-Janadi, A;Alva, AS;Phase Ib/II Clinical Trial of Pembrolizumab With Bevacizumab for Metastatic Renal Cell Carcinoma: BTCRC-GU14-003J. Clin. Oncol.32097091Events (CTCAE; version 4.0). Exploratory Studies. Expression of PD-L1 in archived diagnostic tumor tissue was determined by immunohistochemistry (IHC) using a 22C3 antibody (Qualtek Electronics, Newton, PA). Both a modified ;logy Criteria for AdFull Paper
2020Tolaney, SM;Barroso-Sousa, R;Keenan, T;Li, T;Trippa, L;Vaz-Luis, I;Wulf, G;Spring, L;Sinclair, NF;Andrews, C;Pittenger, J;Richardson, ET;Dillon, D;Lin, NU;Overmoyer, B;Partridge, AH;Van Allen, E;Mittendorf, EA;Winer, EP;Krop, IE;Effect of Eribulin With or Without Pembrolizumab on Progression-Free Survival for Patients With Hormone Receptor-Positive, ERBB2-Negative Metastatic Breast Cancer: A Randomized Clinical TrialJAMA oncology32880602A total of 65 patients (74%) had PD-L1 testing assessed centrally by QualTek using the 22C3 antibody, and results were reported as a modified pro- portion score (MPS), defined as the proportion of cells, includ- ing both tumor and mononuclear inflammFull Paper
2020Zsiros, E;Lynam, S;Attwood, KM;Wang, C;Chilakapati, S;Gomez, EC;Liu, S;Akers, S;Lele, S;Frederick, PJ;Odunsi, K;Efficacy and Safety of Pembrolizumab in Combination With Bevacizumab and Oral Metronomic Cyclophosphamide in the Treatment of Recurrent Ovarian Cancer: A Phase 2 Nonrandomized Clinical TrialJAMA oncology33211063A modified proportion score threshold of at least 1% using the 22C3 antibody (QualTekMolecular Laboratories) was adopted similarly to previously described protocols.6Full Paper
2020Jabbour, SK;Berman, AT;Decker, RH;Lin, Y;Feigenberg, SJ;Gettinger, SN;Aggarwal, C;Langer, CJ;Simone, CB;Bradley, JD;Aisner, J;Malhotra, J;Phase 1 Trial of Pembrolizumab Administered Concurrently With Chemoradiotherapy for Locally Advanced Non-Small Cell Lung Cancer: A Nonrandomized Controlled TrialJAMA Oncol32077891Exploratory analyses included PD-L1 status, by mean proportion score. Immunohistochemical staining for PD-L1 used a mouse monoclonal anti-PD-L1 antibody (clone 22C3).21 Furthermore, TILs were scored on a continuum from 0 to 3 (0 indicates absent TILsFull Paper
2020Kelly, CM;Antonescu, CR;Bowler, T;Munhoz, R;Chi, P;Dickson, MA;Gounder, MM;Keohan, ML;Movva, S;Dholakia, R;Ahmad, H;Biniakewitz, M;Condy, M;Phelan, H;Callahan, M;Wong, P;Singer, S;Ariyan, C;Bartlett, EK;Crago, A;Yoon, S;Hwang, S;Erinjeri, JP;Qin, LX;Tap, WD;D'Angelo, SP;Objective Response Rate Among Patients With Locally Advanced or Metastatic Sarcoma Treated With Talimogene Laherparepvec in Combination With Pembrolizumab: A Phase 2 Clinical TrialJAMA Oncol31971541Paraffin-embedded tumor material taken before and after treatment was examined for TIL immune biomarker expression, including PD-L1, PD-1, CD3, CD4, FOXP3, CD8, CD68, CD163, and MHC1 by Qualtek Laboratory. Programmed death-ligand 1 tumor membranous eFull Paper
2020Mancuso, F;Ogbah, Z;Fasani, R;Lo Giacco, D;Ramos, L;Oaknin, A;Alsina, M;Elez, E;Mercade, T;Saura, C;Capdevila, J;Vieito, M;Felip, E;Dienstmann, R;Gros, A;Seoane, J;Nuciforo, P;Tabernero, J;Garralda, E;Vivancos, A;581P VHIO immune gene expression profiling (VIGex) panel, a tool to explore tumour immune microenvironmentAnnals of Oncologyself), Speaker Bureau/Expert testimony, Research grant/Funding (self), Travel/Accommodation/ Expenses: Novartis; Honoraria (self), Speaker Bureau/Expert testimony, Research grant/Funding (self), Travel/Accommodation/Expenses: Targos Molecular PatholoFull Paper
2020Jotte, R;Cappuzzo, F;Vynnychenko, I;Stroyakovskiy, D;Rodríguez-Abreu, D;Hussein, M;Soo, R;Conter, HJ;Kozuki, T;Huang, KC;Graupner, V;Sun, SW;Hoang, T;Jessop, H;McCleland, M;Ballinger, M;Sandler, A;Socinski, MA;Atezolizumab in Combination With Carboplatin and Nab-Paclitaxel in Advanced Squamous NSCLC (IMpower131): Results From a Randomized Phase III TrialJ Thorac Oncol32302702Skip to Main Content ;This work was supported by F. Hoffmann-La Roche Ltd/Genentech, Inc., a member of the Roche Group. The authors would like to acknowledge Targos Molecular Pathology GmbH (Kassel, Germany) for performing central PD-L1 and T-efFull Paper
2020M.C.Liu, G.R.Oxnard, E.A.Klein, C.Swanton, M.V.SeidenSensitive and specific multi-cancer detection and localization using methylation signatures in cell-free DNAAnnals of OncologyBackground: Early cancer detection could identify tumors at a time when outcomes are superior and treatment is less morbid. This prospective case-control sub-study (from NCT02889978 and NCT03085888) assessed the performance of targeted methylation anFull Paper
2020Taylor, WC;Comment on "Sensitive and specific multi-cancer detection and localization using methylation signatures in cell-free DNA"" by M. C. Liu et al"Ann. Oncol.32371122cfDNA [n = 2628 (1493 cancer; 1135 non-cancer)], formalin fixed paraffin embedded (FFPE) tumor biopsies (n = 242), and WBCs (n = 70) from the first CCGA sub-study; commercial tissue or cells [n = 227; Discovery Life Sciences (formerly Conversant BiolFull Paper
2020Draz, MS;Vasan, A;Muthupandian, A;Kanakasabapathy, MK;Thirumalaraju, P;Sreeram, A;Krishnakumar, S;Yogesh, V;Lin, W;Yu, XG;Chung, RT;Shafiee, H;Virus detection using nanoparticles and deep neural network-enabled smartphone systemScience advances33328239Deidentified HCV-infected and HBV-infected patient plasma/ serum samples were obtained from Discovery Life Sciences IncFull Paper
2020Chung, HC;Piha-Paul, SA;Lopez-Martin, J;Schellens, JHM;Kao, S;Miller, WH;Delord, JP;Gao, B;Planchard, D;Gottfried, M;Zer, A;Jalal, SI;Penel, N;Mehnert, JM;Matos, I;Bennouna, J;Kim, DW;Xu, L;Krishnan, S;Norwood, K;Ott, PA;Pembrolizumab After Two or More Lines of Previous Therapy in Patients With Recurrent or Metastatic SCLC: Results From the KEYNOTE-028 and KEYNOTE-158 StudiesJ Thorac Oncol31870883In KEYNOTE-028, tumor samples were assessed using a laboratory-developed prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA) with the 22C3 antibody clone (Merck Sharp & Dohme Corp., a subsidiary of Merck & Co., Inc., KenFull Paper
2020Freeman, ZT;Nirschl, TR;Hovelson, DH;Johnston, RJ;Engelhardt, JJ;Selby, MJ;Kochel, CM;Lan, RY;Zhai, J;Ghasemzadeh, A;Gupta, A;Skaist, AM;Wheelan, SJ;Jiang, H;Pearson, AT;Snyder, LA;Korman, AJ;Tomlins, SA;Yegnasubramanian, S;Drake, CG;A conserved intratumoral regulatory T cell signature identifies 4-1BB as a pan-cancer targetJ. Clin. Invest.32015231Study approval. All human tumors were collected with approval from the Johns Hopkins University IRB with the following protocol IDs: renal clear cell carcinoma (IRB00033839), prostate cancer (NA_00082175), bladder cancer (NA_00026693), and glioblastoFull Paper
2020Weiss, J;Sheth, S;Deal, AM;Grilley Olson, JE;Patel, S;Hackman, TG;Blumberg, JM;Galloway, T;Patel, S;Zanation, AM;Shen, CJ;Hayes, DN;Hilliard, C;Mehra, R;McKinnon, KP;Wang, HH;Weissler, MC;Bauman, JR;Chera, BS;Vincent, BG;Concurrent definitive immunoradiotherapy for patients with Stage III-IV Head and Neck Cancer and Cisplatin contraindicationClin. Cancer Res.32371539withdrawal of permission. Correlative Analysis PDL1 staining was conducted by Qualtek Molecular Laboratories (Newtown, PA) as previously described 5 and required by Merck in supported investigator initiated trials. For bothFull Paper
2020Graff, JN;Beer, TM;Alumkal, JJ;Slottke, RE;Redmond, WL;Thomas, GV;Thompson, RF;Wood, MA;Koguchi, Y;Chen, Y;Latour, E;Bergan, RC;Drake, CG;Moran, AE;A phase II single-arm study of pembrolizumab with enzalutamide in men with metastatic castration-resistant prostate cancer progressing on enzalutamide aloneJ Immunother Cancer32616555retained. Formalin-fixed, paraffin-embedded tumor samples were sent to a commercial vendor (Qualtek) for PD-L1 quantification by immunohistochemistry (clone 22C3). Additional methods details are described elsewhere.30Full Paper
2020Majd, N;Waguespack, S;Janku, F;Fu, S;Penas-Prado, M;Xu, M;Alshawa, A;Kamiya-Matsuoka, C;Raza, S;McCutcheon, I;Naing, A;Efficacy of pembrolizumab in patients with pituitary carcinoma: report of four cases from a phase II studyJournal for ImmunoTherapy of CancerPD-L1 staining was performed by Qualtek using Merck 22C3 antibody for PD-L1Full Paper
2020Lokeshwar, VB;Morera, DS;Hasanali, SL;Yates, TJ;Hupe, MC;Knapp, J;Lokeshwar, SD;Wang, J;Hennig, MJP;Baskar, R;Escudero, DO;Racine, RR;Dhir, N;Jordan, AR;Hoye, K;Azih, I;Manoharan, M;Klaassen, Z;Kavuri, S;Lopez, LE;Ghosh, S;Lokeshwar, BL;A Novel Splice Variant of HYAL-4 Drives Malignant Transformation and Predicts Outcome in Patients with Bladder CancerClin. Cancer Res.32094233Present address: Travis Yates: QualTek Molecular Laboratories, King of Prussia, PA; Diogo Escudero: George Washington University Kelly Hoye: University of North Carolina Lineberger Comprehensive Cancer Center; Ronny Racine: Astellas Institute for RegFull Paper
2020Fong, LY;Taccioli, C;Palamarchuk, A;Tagliazucchi, GM;Jing, R;Smalley, KJ;Fan, S;Altemus, J;Fiehn, O;Huebner, K;Farber, JL;Croce, CM;Abrogation of esophageal carcinoma development in miR-31 knockout ratsProc. Natl. Acad. Sci. U.S.A.32123074lia isolated using the DNeasy Blood & Tissue Kit (Qiagen). WGS. DNA library preparation, library quality control, and nonhuman WGS to yield ∼30× coverage were performed on the Illumina HiSeq X at HudsonAlpha Genomic Services Laboratory. WGS AnalysiFull Paper
2020Rodriguez, CP;Wu, QV;Voutsinas, J;Fromm, JR;Jiang, X;Pillarisetty, VG;Lee, SM;Santana-Davila, R;Goulart, B;Baik, CS;Chow, LQM;Eaton, K;Martins, R;A Phase II Trial of Pembrolizumab and Vorinostat in Recurrent Metastatic Head and Neck Squamous Cell Carcinomas and Salivary Gland CancerClin. Cancer Res.31796519Submission of archived tissue was required prior to study entry. In patient with no available archived tissue, fresh tissue biopsies were recommended. Biopsies were attempted after three cycles of therapy were completed, in patients who consented. PDFull Paper
2020Huetter, J;Gritzan, U;Gutcher, I;Doecke, WD;Luetke-Eversloh, MV;Golfier, S;Roider, HG;Frisk, AL;Hunter, J;Pow, A;Drake, A;Levine, Z;Levy, O;Azulay, M;Barbiro, I;Cojocaru, G;Vaknin, I;Kreft, B;Roese, L;Characterization of BAY 1905254, an immune checkpoint inhibitor targeting the immunoglobulin-like domain containing receptor 2 (ILDR2)Cancer Immunol Res32312711cancer patients, and commercially available cryopreserved human lymph node single- cell preparations from melanoma patients (Folio Conversant, USA) using the PrimeFlow™ RNA Assay technology (Invitrogen, CA, USA). Following enzymaticFull Paper
2020Ozkan-Dagliyan, I;Diehl, JN;George, SD;Schaefer, A;Papke, B;Klotz-Noack, K;Waters, AM;Goodwin, CM;Gautam, P;Pierobon, M;Peng, S;Gilbert, TSK;Lin, KH;Dagliyan, O;Wennerberg, K;Petricoin, EF;Tran, NL;Bhagwat, SV;Tiu, RV;Peng, SB;Herring, LE;Graves, LM;Sers, C;Wood, KC;Cox, AD;Der, CJ;Low-Dose Vertical Inhibition of the RAF-MEK-ERK Cascade Causes Apoptotic Death of KRAS Mutant CancersCell Rep32553168CRISPR sequencing was performed at UNC (High-Throughput Sequencing Facility, Chapel Hill, NC) and Hudson Alpha (HudsonAlpha Genomic Services Laboratory, Huntsville, AL)Full Paper
2020Wang, X;Kelkar, YD;Xiong, X;Martinson, EO;Lynch, J;Zhang, C;Werren, JH;Wang, X;Genome Report: Whole Genome Sequence and Annotation of the Parasitoid Jewel Wasp Nasonia giraulti Laboratory Strain RV2X[u]G3 (Bethesda)32571804and Helen Wisch Professorship. X.X. is supported by the Auburn University Presidential Graduate Research Fellowship and Auburn University College of Veterinary Medicine Dean’s Fellowship. We thank HudsonAlpha Discovery for assistance with Illumina seFull Paper
2020Ulasov, I;Borovjagin, A;Fares, J;Yakushov, S;Malin, D;Timashev, P;Lesniak, MS;MicroRNA 345 (miR345) regulates KISS1-E-cadherin functional interaction in breast cancer brain metastasesCancer Lett.32246957One hundred and eleven tumor samples, representing invasive ductal carcinoma (IDC) were subjected for evaluation as a part of brain tissue microarrays presented by: US Biomax (BR812), Folio Biosciences (AY-HH0137) and biobank at the University of ChiFull Paper
2020Chiang, J;Ahmed, N;Foote, J;Prasad, N;Karri, S;Pagidas, K;Wiltshire, A;Gunn, D;Singh, K;OVARIAN AGING AND REPRODUCTIVE SENESCENCE IN MOUSE MODEL OF MITOCHONDRIAL DYSFUNCTIONFertility and Sterility2 HudsonAlpha Discovery, Huntsville, AL; 3 TCM University, Lincoln, RIFull Paper
2020Ho, AY;Barker, CA;Arnold, BB;Powell, SN;Hu, ZI;Gucalp, A;Lebron-Zapata, L;Wen, HY;Kallman, C;D'Agnolo, A;Zhang, Z;Flynn, J;Dunn, SA;McArthur, HL;A phase 2 clinical trial assessing the efficacy and safety of pembrolizumab and radiotherapy in patients with metastatic triple-negative breast cancerCancer31747077PD‐L1 tumor expression was evaluated in prospectively collected samples of metastatic biopsies by a central laboratory (QualTek Molecular Laboratories, Goleta, California) using a previously described prototype assay using the 22C3 antibody (Merck anFull Paper
2020Kasimir-Bauer, S;Roder, J;Obermayr, E;Mahner, S;Vergote, I;Loverix, L;Braicu, E;Sehouli, J;Concin, N;Kimmig, R;Net, L;Roder, H;Zeillinger, R;Aust, S;On Behalf Of The Ovcad Ovarian Cancer Diagnosis Initiative, ;Definition and Independent Validation of a Proteomic-Classifier in Ovarian CancerCancers32899818These samples were obtained from commercial biobanks (Conversant Bio (Huntsville, AL, USA) and Oncology Metrics (Forth Worth, TX, USA)), under their ethics-approved protocolsFull Paper
2020Jimenez, C;Subbiah, V;Stephen, B;Ma, J;Milton, D;Xu, M;Zarifa, A;Akhmedzhanov, FO;Tsimberidou, A;Habra, MA;Rodon Anhert, J;Fu, S;Naing, A;Phase II Clinical Trial of Pembrolizumab in Patients with Progressive Metastatic Pheochromocytomas and ParagangliomasCancers32824391tissue samples or newly obtained biopsy specimens (if archival tissue was not available) were evaluated by immunohistochemical analysis to determine the expression of PDL-1 in tumor cells and tumor-infiltrating mononuclear inflammatory cells by QualtFull Paper
2020Jordan, AR;Wang, J;Yates, TJ;Hasanali, SL;Lokeshwar, SD;Morera, DS;Shamaladevi, N;Li, CS;Klaassen, Z;Terris, MK;Thangaraju, M;Singh, AB;Soloway, MS;Lokeshwar, VB;Molecular targeting of renal cell carcinoma by an oral combinationOncogenesis32427869Department of Biochemistry and Molecular Biology, Medical College of Georgia, Augusta University, 1410 Laney Walker Blvd., Augusta, GA, 30912, USA.||Sheila and David Fuente Graduate Program in Cancer Biology, University of Miami-Miller School of MediFull Paper
2020Mehnert, JM;Bergsland, E;O'Neil, BH;Santoro, A;Schellens, JHM;Cohen, RB;Doi, T;Ott, PA;Pishvaian, MJ;Puzanov, I;Aung, KL;Hsu, C;Le Tourneau, C;Hollebecque, A;Élez, E;Tamura, K;Gould, M;Yang, P;Stein, K;Piha-Paul, SA;Pembrolizumab for the treatment of programmed death-ligand 1-positive advanced carcinoid or pancreatic neuroendocrine tumors: Results from the KEYNOTE-028 studyCancer32320048PD was confirmed by repeat radiographic imaging ≥4 weeks after the initial scan. Investigators could opt to keep clinically stable patients on treatment until confirmed PD. Safety was monitored through 30 days after the last treatment dose (90 days fFull Paper
2020Wen, X;Ou, Y;Zarick, H;Zhang, X;Hmelo, A;Victor, Q;Paul, E;Slocik, J;Naik, R;Bellan, L;Lin, E;Bardhan, R;PRADA: Portable Reusable Accurate Diagnostics with nanostar Antennas for multiplexed biomarker screeningBioeng Transl Medlabor intensive, and cost prohibitive.39,40 We chose to measure the accuracy of PRADA in commercially available de-identified human patient serum (Discovery Life Sciences Inc.) to recapitulate clinical diagnostics where biomarkers of interest competeFull Paper
2020Gonzalez-Ericsson, PI;Stovgaard, ES;Sua, LF;Reisenbichler, E;Kos, Z;Carter, JM;Michiels, S;Le Quesne, J;Nielsen, TO;Laenkholm, AV;Fox, SB;Adam, J;Bartlett, JM;Rimm, DL;Quinn, C;Peeters, D;Dieci, MV;Vincent-Salomon, A;Cree, I;Hida, AI;Balko, JM;Haynes, HR;Frahm, I;Acosta-Haab, G;Balancin, M;Bellolio, E;Yang, W;Kirtani, P;Sugie, T;Ehinger, A;Castaneda, CA;Kok, M;McArthur, H;Siziopikou, K;Badve, S;Fineberg, S;Gown, A;Viale, G;Schnitt, SJ;Pruneri, G;Penault-Llorca, F;Hewitt, S;Thompson, EA;Allison, KH;Symmans, WF;Bellizzi, AM;Brogi, E;Moore, DA;Larsimont, D;Dillon, DA;Lazar, A;Lien, H;Goetz, MP;Broeckx, G;El Bairi, K;Harbeck, N;Cimino-Mathews, A;Sotiriou, C;Adams, S;Liu, SW;Loibl, S;Chen, IC;Lakhani, SR;Juco, JW;Denkert, C;Blackley, EF;Demaria, S;Leon-Ferre, R;Gluz, O;Zardavas, D;Emancipator, K;Ely, S;Loi, S;Salgado, R;Sanders, M;, ;The path to a better biomarker: application of a risk management framework for the implementation of PD-L1 and TILs as immuno-oncology biomarkers in breast cancer clinical trials and daily practiceJ. Pathol.32129476PANACEA NCT02129556 58 Pembrolizumab + trastuzumab single arm phase Ib/II PreTx LAdv or mHER2+ BC Ib: PD‐L1+ (6) II: PD‐L1+ & PD‐L1‐ (52) PD‐L1 (QualTek/ 22C3) tested prospectively at BTx (58). TILs were evaluated retrospectively (48). II: HFull Paper
2020Lu, H;Molony, RD;Chen, D;Jang, S;Wolf, B;Ewert, S;Flaherty, M;Xu, F;Isim, S;Shim, Y;Dornelas, C;Balke, N;Leber, XC;Scharenberg, M;Koelln, J;Choi, E;Ward, R;Johnson, J;Calzascia, T;Isnardi, I;Williams, JA;Lindenbergh, PL;van de Donk, NWCJ;Mutis, T;Huet, H;Lees, E;Meyer, MJ;Development of anti-CD32b antibodies with enhanced Fc function for the treatment of B and plasma cell malignanciesMol. Cancer Ther.32847974detect CD32b expression on primary cells and cell lines. Patient samples for CD32b expression analysis were purchased from Conversant Bio, now part of Discovery Life Science. 2B6 binding to normal human plasma cells fromFull Paper
2020Hutchins, B;Starling, GC;McCoy, MA;Herzyk, D;Poulet, FM;Dulos, J;Liu, L;Kang, SP;Fayadat-Dilman, L;Hsieh, M;Andrews, CL;Ayanoglu, G;Cullen, C;Malefyt, RW;Kastelein, RA;Saux, SL;Lee, J;Li, S;Malashock, D;Sadekova, S;Soder, G;van Eenennaam, H;Willingham, A;Yu, Y;Streuli, M;Carven, GJ;van Elsas, A;Biophysical and Immunological Characterization and In Vivo Pharmacokinetics and Toxicology in Nonhuman Primates of the Anti-PD-1 Antibody PembrolizumabMolecular cancer therapeutics32229606See Supplementary Data for additional details. Primary tumor cell culture assay Fresh non-small cell lung cancer (NSCLC) tumor tissues were collected and shipped overnight in AQIX media (AQIX) by collection providers (Folio Biosciences, Bio-Options,Full Paper
2020Smith, SD;Till, BG;Shadman, MS;Lynch, RC;Cowan, AJ;Wu, QV;Voutsinas, J;Rasmussen, HA;Blue, K;Ujjani, CS;Shustov, A;Cassaday, RD;Fromm, JR;Gopal, AK;Pembrolizumab with R-CHOP in previously untreated diffuse large B-cell lymphoma: potential for biomarker driven therapyBr. J. Haematol.32030732PD‐L1 immunohistochemistry was performed centrally by QualTek Molecular Laboratories using the mouse monoclonal antibody clone 22c3 by previously described methods (Supplementary Appendix, Section B) (Dolled‐Filhart et al. , 2016). Statistical considFull Paper
2020Vijayvergia, N;Dasari, A;Deng, M;Litwin, S;Al-Toubah, T;Alpaugh, RK;Dotan, E;Hall, MJ;Ross, NM;Runyen, MM;Denlinger, CS;Halperin, DM;Cohen, SJ;Engstrom, PF;Strosberg, JR;Pembrolizumab monotherapy in patients with previously treated metastatic high-grade neuroendocrine neoplasms: joint analysis of two prospective, non-randomised trialsBr. J. Cancer32152503For patients enrolled into FC, PD-L1 immunohistochemistry (IHC) assay was performed at Qualtek Research Laboratories on formalin-fixed, paraffin-embedded tissue sections using anti-PD-L1 monoclonal antibody clone 22C3 [Merck Research Laboratories]Full Paper
2020Schruf, E;Schroeder, V;Le, HQ;Schönberger, T;Raedel, D;Stewart, EL;Fundel-Clemens, K;Bluhmki, T;Weigle, S;Schuler, M;Thomas, MJ;Heilker, R;Webster, MJ;Dass, M;Frick, M;Stierstorfer, B;Quast, K;Garnett, JP;Recapitulating idiopathic pulmonary fibrosis related alveolar epithelial dysfunction in a human iPSC-derived air-liquid interface modelFASEB J.32297676Formalin-fixed and paraffin embedded lung samples from human IPF patients were purchased from Folio Biosciences (Powell, OH, US) under the regulatory conditions of the Boehringer Ingelheim corporate policy regarding the acquisition and use of human bFull Paper
2020Kang, J;Yeom, G;Jang, H;Park, C;Kim, M;Highly sensitive and universal detection strategy based on a colorimetric assay using target-specific heterogeneous sandwich DNA aptamerAnalytica Chimica ActaAll reagent concentrations were the same as those analyzed in the developed method. 2.8. Evaluation of clinical samples. We obtained patient samples containing NPs of influenza A and B viruses from Discovery Life Sciences, Inc (Huntsville, AL, USA)Full Paper
2020Seyger, M;Abramovits, W;Liljedahl, M;Hoejen, MN;Teng, J;Safety and efficacy of fixed-dose combination calcipotriol (50 μg/g) and betamethasone dipropionate (0.5 mg/g) cutaneous foam in adolescent patients (aged 12 to <17 years) with plaque psoriasis: results of a phase II, open-label trialJ Eur Acad Dermatol Venereol32074665W. Abramovits has received honoraria or fees for serving on advisory boards as a speaker and as a consultant, and has received grants as an investigator from AbbVie, Akros, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant BioFull Paper
2020Piha-Paul, SA;Oh, DY;Ueno, M;Malka, D;Chung, HC;Nagrial, A;Kelley, RK;Ros, W;Italiano, A;Nakagawa, K;Rugo, HS;de Braud, F;Varga, AI;Hansen, A;Wang, H;Krishnan, S;Norwood, KG;Doi, T;Efficacy and safety of pembrolizumab for the treatment of advanced biliary cancer: Results from the KEYNOTE-158 and KEYNOTE-028 studiesInt. J. Cancer32359091In KEYNOTE‐028, a prototype immunohistochemistry assay (QualTek, Goleta, California) was used to prospectively detect PD‐L1 expression, with positivity defined as membranous PD‐L1 expression in ≥1% of tumor and associated inflammatory cells or positiFull Paper
2020Agnello, G;Alters, SE;Rowlinson, SW;Preclinical safety and antitumor activity of the arginine-degrading therapeutic enzyme pegzilarginase, a PEGylated, cobalt-substituted recombinant human arginase 1Transl Res31954097CD8 IHC analysis of FFPE sections was performed by QualTek Molecular Laboratories (Goleta, Calif) and LC3B IHC analysis of FFPE sections was performed by GoPath Laboratories (Buffalo Grove, Ill). Statistical analysesFull Paper
2020Ross, AE;Hurley, PJ;Tran, PT;Rowe, SP;Benzon, B;Neal, TO;Chapman, C;Harb, R;Milman, Y;Trock, BJ;Drake, CG;Antonarakis, ES;A pilot trial of pembrolizumab plus prostatic cryotherapy for men with newly diagnosed oligometastatic hormone-sensitive prostate cancerProstate Cancer Prostatic Dis.31611635pathologist. Post-treatment biopsies were evaluated for PD-L1, CD4, CD8, and FoxP3 proteins by single-stain semiquantitative immunohistochemistry performed by QualTek Molecular and Clinical Laboratories (Santa Barbara, CA) ;re assessed by a geniFull Paper
2020Scheffler, M;Holzem, A;Kron, A;Nogova, L;Ihle, MA;von Levetzow, C;Fassunke, J;Wömpner, C;Bitter, E;Koleczko, S;Abdulla, DSY;Michels, S;Fischer, R;Riedel, R;Weber, JP;Westphal, T;Gerigk, U;Kern, J;Kaminsky, B;Randerath, W;Kambartel, KO;Merkelbach-Bruse, S;Büttner, R;Wolf, J;Co-occurrence of targetable mutations in Non-small cell lung cancer (NSCLC) patients harboring MAP2K1 mutationsLung Cancer32361034Reinhard Büttner: RB has been a consultant for Pfizer and Novartis regarding MET inhibitors and is a co-founder and CSO of Targos Molecular Pathology, Kassel GermanyFull Paper
2020Lee, EK;Xiong, N;Cheng, SC;Barry, WT;Penson, RT;Konstantinopoulos, PA;Hoffman, MA;Horowitz, N;Dizon, DS;Stover, EH;Wright, AA;Campos, SM;Krasner, C;Morrissey, S;Whalen, C;Quinn, R;Matulonis, UA;Liu, JF;Combined pembrolizumab and pegylated liposomal doxorubicin in platinum resistant ovarian cancer: A phase 2 clinical trialGynecol. Oncol.32771276Tumor infiltrating lymphocytes (TILs) were estimated as an overall average of the sample and reported on a scale of 0 (absent TILs) to 3 (profuse infiltration). PD-L1 and TIL analysis was performed at QualTek Molecular Laboraties, PAFull Paper
2020Frumovitz, M;Westin, SN;Salvo, G;Zarifa, A;Xu, M;Yap, TA;Rodon, AJ;Karp, DD;Abonofal, A;Jazaeri, AA;Naing, A;Phase II study of pembrolizumab efficacy and safety in women with recurrent small cell neuroendocrine carcinoma of the lower genital tractGynecol. Oncol.32534809Adverse events were assessed using the National Cancer Institute Common Terminology Criteria for Adverse Events version 4.03. When fresh or archived tissue was available, PD-L1 biomarker analysis was performed by Qualtek assay using Merck 22C3 antiboFull Paper
2020Gaillard, S;Broadwater, G;Andrews, W;Starr, M;Previs, R;Havrilesky, L;Davidson, B;Berchuck, A;Yi, J;Nixon, A;Secord, A;Pembrolizumab window study: Illuminating the immunologic landscape in gynecologic cancersGynecologic Oncologychanges in PD-L1 expression (by immunohistochemistry with monoclonal antibody clone 22C3 on FFPE specimens;l Qualtek, PA) scored using a quantitative modified proportion score (MPS)Full Paper
2020Pillarisetti, K;Powers, G;Luistro, L;Babich, A;Baldwin, E;Li, Y;Zhang, X;Mendonça, M;Majewski, N;Nanjunda, R;Chin, D;Packman, K;Elsayed, Y;Attar, R;Gaudet, F;Teclistamab is an active T cell-redirecting bispecific antibody against B-cell maturation antigen for multiple myelomaBlood Adv32956453Plasma samples were from normal subjects (n = 39) and patients with MM (n = 40); donors were acquired from Conversant Biologics and subjected to proteolysis and subsequent mass spectrometry using a reference multiple reaction monitoring sequence (YCNFull Paper
2020Svrzikapa, N;Longo, K;Prasad, N;Boyanapalli, R;Brown, J;Dorset, D;Yourstone, S;Powers, J;Levy, S;Morris, A;Vargeese, C;Goyal, J;Investigational assay for haplotype phasing of the huntingtin geneMolecular Therapy - Methods & Clinical Development4 1 Wave Life Sciences Ltd., Cambridge, MA, 02138 USA; 2 Department of Paediatrics, Medical 5 Sciences Division, University of Oxford, Oxford, OX3 9DU UK; 3 HudsonAlpha Discovery, 6 Discovery Life Sciences, Huntsville, AL, 35806 USA; 4 Genomic ServicFull Paper
2020Sebest, P;Fojt, L;Ostatna, V;Fojta, M;Danhel, A;Electrodeposited silver amalgam particles on pyrolytic graphite in (spectro)electrochemical detection of 4-nitrophenol, DNA and green fluorescent proteinBioelectrochemistry31855832electrode setup composed of: i) a bPGE or edge-plane PGE (ePGE, both Momentive) working electrode, together with ii) a refillable miniature Ag/AgCl/3M KCl reference electrode (eDAQ) placed into the salt bridge composed of a heat shrink Teflon tube (QFull Paper
2020Xicoy, H;Brouwers, JF;Wieringa, B;Martens, GJM;Explorative Combined Lipid and Transcriptomic Profiling of Substantia Nigra and Putamen in Parkinson's DiseaseCells32858884Total RNA samples were spectrophotometrically analyzed and their 260/280 ratio was typically above 1.9. RNA-seq directionality library preparation and sequencing with Poly (A) selection was performed by HudsonAlpha Genomic Services Laboratory (HuntsvFull Paper
2020Elgharably, H;Okamoto, T;Ayyat, KS;Niikawa, H;Meade, S;Farver, C;Chan, ER;Aldred, MA;McCurry, KR;Human Lungs Airway Epithelium Upregulate MicroRNA-17 and MicroRNA-548b in Response to Cold Ischemia and Ex Vivo ReperfusionTransplantation32590607We thank Tracey Bonfield, PhD from CWRU for performing the cytokines analysis. We thank Gerard J. Nuovo, MD, and Adel Mikhail, PhD from Phylogeny Inc. for performing the in situ hybridization assays of the microRNAFull Paper
2020Grigorieva, J;Asmellash, S;Net, L;Tsypin, M;Roder, H;Roder, J;Mass Spectrometry-Based Multivariate Proteomic Tests for Prediction of Outcomes on Immune Checkpoint Blockade Therapy: The Modern Analytical ApproachInt J Mol Sci3201294150] PSEA reference set NSCLC patients; samples obtained from commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrix (Fort Worth, TX) 100 Grigorieva et al., 2019 [51] 3. BDX008 Test and ICB Test BDX008Full Paper
2020Awty-Carroll, D;Ravella, S;Clifton-Brown, J;Robson, P;Using a Taguchi DOE to investigate factors and interactions affecting germination in Miscanthus sinensisSci Rep32005862All dishes were prepared and tested at the same time to limit extraneous sources of variation. The Taguchi design of experiment (DOE) was produced and primarily analysed using Qualtek-4 (Nutek inc., Michigan, USA) with secondary analysis in R34 ;FulFull Paper
2020Noto, PB;Sikorski, TW;Zappacosta, F;Wagner, CD;Montes de Oca, R;Szapacs, ME;Annan, RS;Liu, Y;McHugh, CF;Mohammad, HP;Piccoli, SP;Creasy, CL;Identification of hnRNP-A1 as a pharmacodynamic biomarker of type I PRMT inhibition in blood and tumor tissuesScientific reports33335114nd Treatment of Laboratory Animals and were reviewed by the Institutional Animal Care and Use Committee at GSK. Immunohistochemistry Both total and ADM-R225-hnRNP-A1 IHC assays were developed and at QualTek Molecular Laboratories using anti-hnRNP-A1Full Paper
2020Rüschoff, J;Lebeau, A;Sinn, P;Schildhaus, HU;Decker, T;Ammann, J;Künzel, C;Koch, W;Untch, M;Statistical modelling of HER2-positivity in breast cancer: Final analyses from two large, multicentre, non-interventional studies in GermanyBreast31918324Institut für Pathologie Nordhessen and Targos Molecular Pathology GmbH, Germaniastr. 7, D-34119, Kassel, Germany. Electronic address: rueschoff@patho-nordhessen.de.++Institut für Pathologie, Universitätsklinikum Hamburg-Eppendorf, Martinistr. 52, 202Full Paper
2020Emancipator, K;Keytruda and PD-L1: a Real-World Example of Co-development of a Drug with a Predictive BiomarkerThe AAPS journal33222057two clinical trial assays were used (Table I). The first, described in the literature as the "prototype assay" (4), was developed in-house at Merck & Co., Inc., Kenilworth, NJ, USA, and eventually placed and refined in an accredited clinical laboratoFull Paper
2020Gebauer, F;Krämer, M;Bruns, C;Schlößer, HA;Thelen, M;Lohneis, P;Schröder, W;Zander, T;Alakus, H;Buettner, R;Loeser, H;Quaas, A;Lymphocyte activation gene-3 (LAG3) mRNA and protein expression on tumour infiltrating lymphocytes (TILs) in oesophageal adenocarcinomaJ. Cancer Res. Clin. Oncol.32592066Reinhard Büttner: Co-Founder and Co-Owner of Targos Molecular Pathology Inc. Kassel/Germany. Hans Schlösser: Funding for Research from Astra Zeneca for research outside this study. Thomas Zander: BMS: advisory board, clinical trial; Novartis: advisorFull Paper
2020Wang, X;Jaimes, M;Gu, H;Shults, K;Putta, S;Sharma, V;Chow, W;Gogoi, P;Handique, K;Patterson, BK;Cell by cell immuno- and cancer marker profiling of non-small cell lung cancer tissue: Checkpoint marker expression on CD103+, CD4+ T-cells predicts circulating tumor cellsTranslational oncology33217647of a total of 72 biomarkers that result from using combinations of cellular markers in our algorithm to predict CTCs. Material and methods Samples Fresh tissues were obtained from 10 NSCLC cases by Folio Biosciences [Powell, OH] with paired peripherFull Paper
2020MacCarty, N;Bentson, S;Cushman, K;Au, J;Li, C;Murugan, G;Still, D;Stratification of particulate matter in a kitchen: A comparison of empirical to predicted concentrations and implications for cookstove emissions targetsEnergy for Sustainable Developmentthe top perimeter (Fig. 3). Small computer fans (Qualtek FAD1-06020CSHW11 rated at 1.44 W @12 V and 16 CFM) covered the holes at the top perimeter to force air out of the test kitchen at a constant rate. In Phase 1 of theFull Paper
2020Jia, Y;Liu, L;Shan, B;Future of immune checkpoint inhibitors: focus on tumor immune microenvironmentAnnals of translational medicine33145314The TNBC cohort in KEYNOTE-012 phase Ib trial enrolled 32 cases of heavily pre-treated patients with PD-L1 positivity in the stroma or PD-L1 ≥1% tumor cells using their Qualtek assayFull Paper
2020Sebest, P;Ostatna, V;Fojta, M;Danhel, A;Constant-current chronopotentiometric stripping detection of bovine serum albumin on silver amalgam particlesJournal of Electroanalytical Chemistryin three-electrode setup composed of: i) base plane PGE (bPGE,od 3.0 mm, Momentive) as working electrode, together with ii) refillable miniature Ag/AgCl/3 M KCl reference electrode (eDAQ) placed into the salt bridge composed of heat shrink Teflon tubFull Paper
2020Rehkaemper, J;Korenkov, M;Quaas, A;Rueschoff, J;Pamuk, A;Zander, T;Hillmer, AM;Buettner, R;Hoelscher, AH;Bruns, CJ;Loeser, H;Alakus, H;Schoemig-Markiefka, B;Amplification of KRAS and its heterogeneity in non-Asian gastric adenocarcinomasBMC Cancer32571252Institute of Pathology, University Hospital Cologne, Cologne, Germany. jan.rehkaemper@uk-koeln.de.++Department of General, Visceral and Cancer Surgery, University Hospital Cologne, Cologne, Germany.++Institute of Pathology, University Hospital ColognFull Paper
2020Montalban-Bravo, G;Class, CA;Ganan-Gomez, I;Kanagal-Shamanna, R;Sasaki, K;Richard-Carpentier, G;Naqvi, K;Wei, Y;Yang, H;Soltysiak, KA;Chien, K;Bueso-Ramos, C;Do, KA;Kantarjian, H;Garcia-Manero, G;Transcriptomic analysis implicates necroptosis in disease progression and prognosis in myelodysplastic syndromesLeukemia31719677BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). Patient characteristics are detailed in Supplementary Table S1. Informed consent was obtained SaveFull Paper
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2020Bio, S;Nunes, B;Acute effects of diclofenac on zebrafish: Indications of oxidative effects and damages at environmentally realistic levels of exposureEnviron. Toxicol. Pharmacol.32320907With the increasing awareness about the contamination of the aquatic environment by pharmaceuticals, there is a growing need to study their adverse effects on aquatic organisms. Diclofenac is a non-steroidal anti-inflammatory drug (NSAID), whose wideFull Paper
2020Piedade, F;Bio, S;Nunes, B;Effects of common pharmaceutical drugs (paracetamol and acetylsalicylic acid) short term exposure on biomarkers of the mussel Mytilus sppEnviron. Toxicol. Pharmacol.31704586Pharmaceutical drugs in the wild may pose significant risks to non-target exposed organisms. This situation is even more troublesome for coastal marine or estuarine environments, located in the vicinity of large human conglomerates, for which the putFull Paper
2020Lin, J;Song, AJ;Hoffman-Censits, J;Leiby, BE;Tuluc, M;Shaw, C;Harshyne, L;Kean, R;Bar-Ad, V;Den, RB;Hurwitz, MD;Louie, J;Philipose, S;Deshmukh, SP;Johnson, JM;Dicker, AP;Hooper, DC;Kelly, WK;Lu, B;A Pilot Study of Radiation Therapy in Combination With Pembrolizumab in Patients With Metastatic Renal Cell CancerAm. J. Clin. Oncol.31693508When possible preradiation and post- radiation biopsy material was compared. Biopsy samples were analyzed by a QualTek pathologist (King of Prussia, PA) and Merck 22C3 antibody was used to score PD-L1 membrane expressionFull Paper
2020Johnston, G;Ramsey, HE;Liu, Q;Wang, J;Stengel, KR;Sampathi, S;Acharya, P;Arrate, M;Stubbs, MC;Burn, T;Savona, MR;Hiebert, SW;Nascent transcript and single-cell RNA-seq analysis defines the mechanism of action of the LSD1 inhibitor INCB059872 in myeloid leukemiaGene32422235Samples were submitted to HudsonAlpha Genomic Services Laboratory for library preparation with Nugen Ovation RNA-Seq System V2 kit (cat# 7102-08) and sequencing on Illumina NovaSeq6000. Data analysis was performed as described above. 2.13 Data availaFull Paper
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2020Barroso-Sousa, R;Krop, IE;Trippa, L;Tan-Wasielewski, Z;Li, T;Osmani, W;Andrews, C;Dillon, D;Richardson, ET;Pastorello, RG;Winer, EP;Mittendorf, EA;Bellon, JR;Schoenfeld, JD;Tolaney, SM;A Phase II Study of Pembrolizumab in Combination With Palliative Radiotherapy for Hormone Receptor-positive Metastatic Breast CancerClin. Breast Cancer32113750Clinical benefit is defined as complete response, partial response, or stable disease ≥ 24 weeks according to RECIST 1.1. Exploratory Biomarkers. Five patients had PD-L1 testing assessed centrally by QualTek using the 22C3 antibodyFull Paper
2020Patel, JJ;Levy, DA;Nguyen, SA;Knochelmann, HM;Day, TA;Impact of PD-L1 expression and human papillomavirus status in anti-PD1/PDL1 immunotherapy for head and neck squamous cell carcinoma-Systematic review and meta-analysisHead Neck31762164Oro Valley, Arizona). One study28 did not specify the assay utilized for PD‐L1 expression, but rather reported the laboratory where analysis occurred (QualTek Molecular Laboratories, Goleta, California). This lab confirmed theFull Paper
2020Adekanmbi, EO;Giduthuri, AT;Srivastava, SK;Dielectric Characterization and Separation Optimization of Infiltrating Ductal Adenocarcinoma via Insulator-DielectrophoresisMicromachines (Basel)32218322The DEP suspending medium (dextrose solution) was prepared and characterized as described in our previous article [38]. The prepared 100 mL suspending medium was divided into five separate beakers. Into each beaker, except the first, calculated volumFull Paper
2020Jasani, B;Bänfer, G;Fish, R;Waelput, W;Sucaet, Y;Barker, C;Whiteley, JL;Walker, J;Hovelinck, R;Diezko, R;Evaluation of an online training tool for scoring programmed cell death ligand-1 (PD-L1) diagnostic tests for lung cancerDiagn Pathol32303234Correspondence: rolf.diezko@targos-gmbh.de 2Advance - Training and Consulting Unit, Targos Molecular Pathology GmbH, Kassel, Germany Full list of author information is available at the end of the article Jasani et al ;Correspondence: rolf.diezkoFull Paper
2020Schöniger, S;Degner, S;Zhang, Q;Schandelmaier, C;Aupperle-Lellbach, H;Jasani, B;Schoon, HA;Tumor Infiltrating Lymphocytes in Pet Rabbit Mammary Carcinomas: A Study with Relevance to Comparative PathologyAnimals : an open access journal from MDPI328245211. Targos Molecular Pathology GmbH, Germaniastrasse 7, 34119 Kassel, Germany. 2. Institute of Veterinary Pathology, University of Leipzig, An den Tierkliniken, 04109 Leipzig, Germany. 3. Institute of Anatomy, Experimental Neurobiology CachedFull Paper
2020Schöniger, S;Schoon, HA;The Healthy and Diseased Equine Endometrium: A Review of Morphological Features and Molecular AnalysesAnimals : an open access journal from MDPI32260515Targos Molecular Pathology GmbH, Germaniastrasse 7, 34119 Kassel, Germany.++Institute of Veterinary Pathology, Faculty of Veterinary Medicine, Leipzig University, An den Tierkliniken 33, 04103 Leipzig, GermanyFull Paper
2020Fish, EJ;Martinez-Romero, EG;DeInnocentes, P;Koehler, JW;Prasad, N;Smith, AN;Bird, RC;Circulating microRNA as biomarkers of canine mammary carcinoma in dogsJ. Vet. Intern. Med.32342546Extracted RNA was stored at −80°C until being shipped on dry ice to the Genomic Services Laboratory at HudsonAlpha Discovery for deep‐sequencing as previously described.14 Briefly, RNA libraries were processed extracted RNA using a NEBNext Small RNAFull Paper
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2020Roder, J;Net, L;Oliveira, C;Meyer, K;Asmellash, S;Kasimir-Bauer, S;Pass, H;Weber, J;Roder, H;Grigorieva, J;A proposal for score assignment to characterize biological processes from mass spectral analysis of serumClinical Mass SpectrometrySamples were obtained from the commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrics (Fort Worth, TX). b They were purchased from commercial biobanks, including Conversant Bio (Huntsville, AL), AdeptBio (Memphis, TN), and ProMedDFull Paper
2020Köhne, M;Mönnig, F;Papin, J;Schöniger, S;Tönissen, A;Rohn, K;Martinsson, G;Schoon, HA;Sieme, H;Effects of Hysteroscopic and Uterine Body Insemination on the Presence of Selected Immune Cells in the Equine EndometriumJ. Equine Vet. Sci.32534786Current affiliation: Targos Molecular Pathology GmbH, 34119 Kassel, GermanyFull Paper
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2020Tsai, AG;Glass, DR;Juntilla, M;Hartmann, FJ;Oak, JS;Fernandez-Pol, S;Ohgami, RS;Bendall, SC;Multiplexed single-cell morphometry for hematopathology diagnosticsNature medicine32161403Healthy bone marrow samples from AllCells were ordered, delivered and processed the same day. Healthy bone marrow 1 and 2 refer to the AllCells 9878 and 10874 samples, SaveFull Paper
2020Reyes, M;Filbin, MR;Bhattacharyya, RP;Billman, K;Eisenhaure, T;Hung, DT;Levy, BD;Baron, RM;Blainey, PC;Goldberg, MB;Hacohen, N;An immune-cell signature of bacterial sepsisNature medicine32066974De-identified BMMC samples were purchased from AllCells or Stemcell Technologies. This study was approved by the Institutional Review Boards at the Broad Institute of MIT and SaveFull Paper
2020Travaglini, KJ;Nabhan, AN;Penland, L;Sinha, R;Gillich, A;Sit, RV;Chang, S;Conley, SD;Mori, Y;Seita, J;Berry, GJ;Shrager, JB;Metzger, RJ;Kuo, CS;Neff, N;Weissman, IL;Quake, SR;Krasnow, MA;A molecular cell atlas of the human lung from single-cell RNA sequencingNature33208946For bulk RNA-seq of canonical immune populations, whole blood from healthy human donors was obtained commericially (AllCells) in EDTA tubes. Patient tissues were obtained under SaveFull Paper
2020Link, JO;Rhee, MS;Tse, WC;Zheng, J;Somoza, JR;Rowe, W;Begley, R;Chiu, A;Mulato, A;Hansen, D;Singer, E;Tsai, LK;Bam, RA;Chou, CH;Canales, E;Brizgys, G;Zhang, JR;Li, J;Graupe, M;Morganelli, P;Liu, Q;Wu, Q;Halcomb, RL;Saito, RD;Schroeder, SD;Lazerwith, SE;Bondy, S;Jin, D;Hung, M;Novikov, N;Liu, X;Villaseñor, AG;Cannizzaro, CE;Hu, EY;Anderson, RL;Appleby, TC;Lu, B;Mwangi, J;Liclican, A;Niedziela-Majka, A;Papalia, GA;Wong, MH;Leavitt, SA;Xu, Y;Koditek, D;Stepan, GJ;Yu, H;Pagratis, N;Clancy, S;Ahmadyar, S;Cai, TZ;Sellers, S;Wolckenhauer, SA;Ling, J;Callebaut, C;Margot, N;Ram, RR;Liu, YP;Hyland, R;Sinclair, GI;Ruane, PJ;Crofoot, GE;McDonald, CK;Brainard, DM;Lad, L;Swaminathan, S;Sundquist, WI;Sakowicz, R;Chester, AE;Lee, WE;Daar, ES;Yant, SR;Cihlar, T;Clinical targeting of HIV capsid protein with a long-acting small moleculeNature32612233Human peripheral blood mononuclear cells (PBMCs) were collected from healthy volunteers under informed consent; their use was approved by an institutional review board at AllCells SaveFull Paper
2020Ferrari, S;Jacob, A;Beretta, S;Unali, G;Albano, L;Vavassori, V;Cittaro, D;Lazarevic, D;Brombin, C;Cugnata, F;Kajaste-Rudnitski, A;Merelli, I;Genovese, P;Naldini, L;Efficient gene editing of human long-term hematopoietic stem cells validated by clonal trackingNature biotechnology32601433(Biovision) and 50 nM UM171 (STEMCell Technologies), unless otherwise specified. G-CSF mPB CD34+ HSPCs were purified with the CliniMACS CD34 Reagent System (Miltenyi Biotec) from Mobilized Leukopak (AllCells) upon approval by the Ospedale San RaffaeFull Paper
2020Spindler, MJ;Nelson, AL;Wagner, EK;Oppermans, N;Bridgeman, JS;Heather, JM;Adler, AS;Asensio, MA;Edgar, RC;Lim, YW;Meyer, EH;Hawkins, RE;Cobbold, M;Johnson, DS;Massively parallel interrogation and mining of natively paired human TCRαβ repertoiresNature biotechnology32393905novel ACT. Online Methods Sourcing and Processing Human Materials De-identified peripheral blood mononuclear cells (PBMCs) in leukopaks were obtained from HLA-A*02:01 and HLA-A*24:02 healthy donors (AllCells, Alameda, CA, USA), under an InstitutionaFull Paper
2020Dinh, HQ;Eggert, T;Meyer, MA;Zhu, YP;Olingy, CE;Llewellyn, R;Wu, R;Hedrick, CC;Coexpression of CD71 and CD117 Identifies an Early Unipotent Neutrophil Progenitor Population in Human Bone MarrowImmunity32814027Human bone marrow cells Fresh bone marrow samples from anonymous healthy adult donors were obtained from AllCells, Inc. (Alameda, CA). For information on donor characteristics, refer to Supplemental Table 1. Upon arrival, the fresh cells were immediaFull Paper
2020Glass, DR;Tsai, AG;Oliveria, JP;Hartmann, FJ;Kimmey, SC;Calderon, AA;Borges, L;Glass, MC;Wagar, LE;Davis, MM;Bendall, SC;An Integrated Multi-omic Single-Cell Atlas of Human B Cell IdentityImmunity32668225structive sleep apnea stanfordhealthcare.org Human lymph node Stanford Hosptial, biopsy sample of recovered lymphoma patient stanfordhealthcare.org Human bone marrow from healthy donors AllCells allcells.com -------------------------Full Paper
2020Park, HB;Wei, Z;Oh, J;Xu, H;Kim, CS;Wang, R;Wyche, TP;Piizzi, G;Flavell, RA;Crawford, JM;Sulfamethoxazole drug stress upregulates antioxidant immunomodulatory metabolites in Escherichia coliNature microbiology32719505ndors supply Eurofins DiscoverX with primary human cells: Cell Applications, Inc., CellzDirect, Celsis-IVT, Leukolab, Life Technologies, Lonza, ScienCell, Hemacare Corporation, Stemcell Technologies, AllCells, Physician’s Plasma Alliance, Lifeline CeFull Paper
2020Di Genua, C;Valletta, S;Buono, M;Stoilova, B;Sweeney, C;Rodriguez-Meira, A;Grover, A;Drissen, R;Meng, Y;Beveridge, R;Aboukhalil, Z;Karamitros, D;Belderbos, ME;Bystrykh, L;Thongjuea, S;Vyas, P;Nerlov, C;C/EBPα and GATA-2 Mutations Induce Bilineage Acute Erythroid Leukemia through Transformation of a Neomorphic Neutrophil-Erythroid ProgenitorCancer cell32330454derbos et al., 2017) ------------------------- Biological Samples ------------------------- AEL patient samples (OX1164; AYL050; MKH048; STB115) MDSBio NA Normal adult human bone marrow AllCells NA ------------------------- CriticalFull Paper
2020Li, S;Chen, X;Wang, J;Meydan, C;Glass, JL;Shih, AH;Delwel, R;Levine, RL;Mason, CE;Melnick, AM;Somatic Mutations Drive Specific, but Reversible, Epigenetic Heterogeneity States in AMLCancer discovery32938585from our prior study(9), 5 of which were purchased from AllCells (Alameda, CA, USA) and 9 of which were isolated using magnetic bead positive selection for CD34+ (Miltenyi Biotec) from freshly collected bone marrow samples from individuals without knFull Paper
2020Zhou, Y;Han, C;Wang, E;Lorch, AH;Serafin, V;Cho, BK;Gutierrez Diaz, BT;Calvo, J;Fang, C;Khodadadi-Jamayran, A;Tabaglio, T;Marier, C;Kuchmiy, A;Sun, L;Yacu, G;Filip, SK;Jin, Q;Takahashi, YH;Amici, DR;Rendleman, EJ;Rawat, R;Bresolin, S;Paganin, M;Zhang, C;Li, H;Kandela, I;Politanska, Y;Abdala-Valencia, H;Mendillo, ML;Zhu, P;Palhais, B;Van Vlierberghe, P;Taghon, T;Aifantis, I;Goo, YA;Guccione, E;Heguy, A;Tsirigos, A;Wee, KB;Mishra, RK;Pflumio, F;Accordi, B;Basso, G;Ntziachristos, P;Posttranslational Regulation of the Exon Skipping Machinery Controls Aberrant Splicing in LeukemiaCancer discovery32444465Our study provides a new proof-of-principle for posttranslational regulation of splicing factors independently of mutations in aggressive T-cell leukemia. It further suggests a new drug SaveFull Paper
2020Tochigi, T;Miyamoto, T;Hatakeyama, K;Sakoda, T;Ishihara, D;Irifune, H;Shima, T;Kato, K;Maeda, T;Ito, T;Handa, H;Akashi, K;Kikushige, Y;Aromatase is a novel neosubstrate of cereblon responsible for immunomodulatory drug-induced thrombocytopeniaBlood32219443Human adult bone marrow and granulocyte colony-stimulating factor-mobilized peripheral blood samples were obtained from healthy donors or purchased from AllCells. Cord blood SaveFull Paper
2020Montalban Bravo, G;Kanagal-Shamanna, R;Darbaniyan, F;Ganan-Gomez, I;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, K;Chien, K;Bueso-Ramos, C;Kantarjian, H;Garcia-Manero, G;Genomic and Transcriptomic Differences of Myelodysplastic Syndrome/Myeloproliferative Neoplasm with Ring Sideroblasts and Thrombocytosis (MDS/MPN-RS-T) and Myelodysplastic Syndrome with Ring Sideroblasts (MDS-RS)BloodCD34+ cells from bone marrow samples of 4 pts with MDS/MPN-RS-T, 7 pts with MDS-RS and 17 healthy individuals obtained from AllCells (Emeryville, CA) were isolated using the SaveFull Paper
2020Montalban Bravo, G;Darbaniyan, F;Kanagal-Shamanna, R;Ganan-Gomez, I;Sasaki, K;Naqvi, K;Wei, Y;Yang, H;Soltysiak, K;Chien, K;Bueso-Ramos, C;Kantarjian, H;Garcia-Manero, G;Transcriptomic Features and Deregulation of Genes Involved in Monopoiesis Influence Outcomes of Patients with Chronic Myelomonocytic LeukemiaBloodINTRODUCTION: Chronic myelomonocytic leukemia (CMML) is characterized by TET2, SRSF2, ASXL1 and RAS pathway mutations known to induce myelomonocytic bias. We have previously shown that upregulation of KDM6B, a histone demethylase that acts as an innaFull Paper
2020Walters, M;Chui, D;Farrell, J;Lal, A;Locatelli, F;Kwiatkowski, J;Porter, J;Sauer, M;Thuret, I;Hongeng, S;Kulozik, A;Thrasher, A;Yannaki, E;Yang, J;Whitney, D;Petrusich, A;Colvin, R;Thompson, A;Response of Patients with Transfusion-Dependent β-Thalassemia (TDT) to Betibeglogene Autotemcel (beti-cel; LentiGlobin for β-Thalassemia) Gene Therapy Based on HBB Genotype and Disease Genetic ModifiersBloodIntroduction We investigated the impact of β-thalassemia genotypes and disease genetic modifiers including HBA and KLF1 genotype and sentinel single-nucleotide polymorphism (SNP) genotypes at 3 major HbF quantitative trait loci (QTL) oFull Paper
2020Kanter, J;Tisdale, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Chen, R;Gallagher, M;Ding, Y;Goyal, S;Paramore, C;Thompson, A;Walters, M;Improvements in Health-Related Quality of Life for Patients Treated with LentiGlobin for Sickle Cell Disease (bb1111) Gene TherapyBloodBackground In patients with sickle cell disease (SCD), health-related quality of life (HRQoL) is worse than in the general population and comparable or worse than in patients with other chronic or painful diseases such as cystic fibrosFull Paper
2020Thompson, A;Kwiatkowski, J;Porter, J;Hongeng, S;Yannaki, E;Kulozik, A;Sauer, M;Thrasher, A;Thuret, I;Lal, A;Guo, R;Liu, W;Colvin, R;Walters, M;Locatelli, F;Favorable Outcomes in Pediatric Patients in the Phase 3 Hgb-207 (Northstar-2) and Hgb-212 (Northstar-3) Studies of Betibeglogene Autotemcel Gene Therapy for the Treatment of Transfusion-Dependent β-ThalassemiaBloodIntroduction Betibeglogene autotemcel (beti-cel; LentiGlobin for β-thalassemia) gene therapy is being evaluated for the treatment of transfusion-dependent β-thalassemia (TDT). Initial positive results of beti-cel in the phase 3 studiesFull Paper
2020Kwiatkowski, J;Walters, M;Hongeng, S;Locatelli, F;Rasko, J;Cavazzana, M;Chen, Y;Colvin, R;Thompson, A;Long-Term Efficacy and Safety of Betibeglogene Autotemcel Gene Therapy for the Treatment of Transfusion-Dependent β-Thalassemia: Results in Patients with up to 6 Years of Follow-upBloodIntroduction The goal of betibeglogene autotemcel (beti-cel; LentiGlobin for β-thalassemia) gene therapy in patients with transfusion-dependent β-thalassemia (TDT) is lifelong, stable production of functional adult hemoglobin (Hb) suffFull Paper
2020Thompson, A;Walters, M;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Aygun, B;Kasow, K;Rifkin-Zenenberg, S;Schmidt, M;DelCarpini, J;Pierciey, F;Miller, A;Gallagher, M;Chen, R;Goyal, S;Kanter, J;Tisdale, J;Resolution of Serious Vaso-Occlusive Pain Crises and Reduction in Patient-Reported Pain Intensity: Results from the Ongoing Phase 1/2 HGB-206 Group C Study of LentiGlobin for Sickle Cell Disease (bb1111) Gene TherapyBloodBackground Sickle cell disease (SCD) is caused by abnormal sickle hemoglobin (HbS) and results in chronic hemolytic anemia, painful vaso-occlusive events (VOEs), and progressive vasculopathy that lead to significant morbidity. While acFull Paper
2020Rothe, K;Babaian, A;Nakamichi, N;Chen, M;Chafe, SC;Watanabe, A;Forrest, DL;Mager, DL;Eaves, CJ;Dedhar, S;Jiang, X;Integrin-Linked Kinase Mediates Therapeutic Resistance of Quiescent CML Stem Cells to Tyrosine Kinase InhibitorsCell stem cell32413332Full Paper
2020Nakao, S;Arai, Y;Tasaki, M;Yamashita, M;Murakami, R;Kawase, T;Amino, N;Nakatake, M;Kurosaki, H;Mori, M;Takeuchi, M;Nakamura, T;Intratumoral expression of IL-7 and IL-12 using an oncolytic virus increases systemic sensitivity to immune checkpoint blockadeScience translational medicine31941828hIL-7/hIL-12-VV at MOI 1.0 and cultured for 2 days. Then, the supernatant was filtered through 0.1-μm polyvinylidene difluoride membrane (Millipore) and added to 1 × 105 human PBMCs (AllCells) in a 96-well plate. Seven days later, secretion of humanFull Paper
2020Geng, J;Liu, W;Zhou, H;Zhang, T;Wang, L;Zhang, M;Li, Y;Shen, B;Li, X;Xiao, B;A Plug-and-Latch Mechanism for Gating the Mechanosensitive Piezo ChannelNeuron32142647HEK293T Xiao et al., 2011 N/A HEK293T-Piezo1-KO Cahalan et al., 2015 N/A HUVEC Allcells Cat# H-001F-C HeLa Xiao et al., 2011 N/A N2A Coste et al., 2010 N/A C2C12 National Infrastructure of Cell line Source, China Cat# GNM26Full Paper
2020Fang, C;Wang, Z;Han, C;Safgren, SL;Helmin, KA;Adelman, ER;Serafin, V;Basso, G;Eagen, KP;Gaspar-Maia, A;Figueroa, ME;Singer, BD;Ratan, A;Ntziachristos, P;Zang, C;Cancer-specific CTCF binding facilitates oncogenic transcriptional dysregulationGenome biology32933554Human CD4 + T cells were purchased from AllCells. Primary human samples were collected with informed consent and analyzed under the supervision of the Institutional Review Board SaveFull Paper
2020Li, M;Liu, W;Bauch, T;Graviss, EA;Arduino, RC;Kimata, JT;Chen, M;Wang, J;Clearance of HIV infection by selective elimination of host cells capable of producing HIVNature communications32792548pecific-pathogen-free barrier animal facility at the Houston Methodist Research Institute. Newborn male and female mice were injected intrahepatically with CD34+ human stem cells (5 × 104/mouse; AllCells LLC). Three months later, reconstitution of huFull Paper
2020Scharenberg, SG;Poletto, E;Lucot, KL;Colella, P;Sheikali, A;Montine, TJ;Porteus, MH;Gomez-Ospina, N;Engineering monocyte/macrophage-specific glucocerebrosidase expression in human hematopoietic stem cells using genome editingNature communications32620863Human CD34+ HSPCs mobilized from peripheral blood were purchased frozen from AllCells (Almeda, CA, USA) and thawed per manufacturer's instructions. Human Cord blood was SaveFull Paper
2020Wienert, B;Nguyen, DN;Guenther, A;Feng, SJ;Locke, MN;Wyman, SK;Shin, J;Kazane, KR;Gregory, GL;Carter, MAM;Wright, F;Conklin, BR;Marson, A;Richardson, CD;Corn, JE;Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repairNature communications32355159Cryopreserved wildtype human mobilized peripheral blood CD34 + HSPCs from volunteer donors were purchased from Allcells, and were cultured in SFEMII + CC110 (StemCell SaveFull Paper
2020Lareau, CA;Ma, S;Duarte, FM;Buenrostro, JD;Inference and effects of barcode multiplets in droplet-based single-cell assaysNature communications32054859lues have been published, these values were approximated from an examination of a plot previously reported2. Profiling PBMCs using 10× scATAC-seq For 10× scATAC-seq experiments with PBMCs (PB003F, Allcells), frozen cells were quickly thawed in a 37Full Paper
2020Mahuron, KM;Moreau, JM;Glasgow, JE;Boda, DP;Pauli, ML;Gouirand, V;Panjabi, L;Grewal, R;Luber, JM;Mathur, AN;Feldman, RM;Shifrut, E;Mehta, P;Lowe, MM;Alvarado, MD;Marson, A;Singer, M;Wells, J;Jupp, R;Daud, AI;Rosenblum, MD;Layilin augments integrin activation to promote antitumor immunityThe Journal of experimental medicine32539073Human peripheral blood mononuclear cells (PBMCs) from two individual donors were purchased from AllCells. CD8 + T cells were enriched from these samples using a negative SaveFull Paper
2020Poran, A;Scherer, J;Bushway, ME;Besada, R;Balogh, KN;Wanamaker, A;Williams, RG;Prabhakara, J;Ott, PA;Hu-Lieskovan, S;Khondker, ZS;Gaynor, RB;Rooney, MS;Srinivasan, L;Combined TCR Repertoire Profiles and Blood Cell Phenotypes Predict Melanoma Patient Response to Personalized Neoantigen Therapy plus Anti-PD-1Cell reports. Medicine33294862Patient PBMCs from NT-001 trial Ott et al., 2020 N/A Healthy Donor PBMCs Precision for Medicine Cat # 93000-10M Healthy Donor PBMCs StemExpress Cat # LE010F Healthy Donor PBMCs AllCells https://www.allcells.com/tissue-products/Full Paper
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2020Poran, A;Harjanto, D;Malloy, M;Arieta, CM;Rothenberg, DA;Lenkala, D;van Buuren, MM;Addona, TA;Rooney, MS;Srinivasan, L;Gaynor, RB;Sequence-based prediction of SARS-CoV-2 vaccine targets using a mass spectrometry-based bioinformatics predictor identifies immunogenic T cell epitopesGenome medicine32791978Human PBMCs from HLA-A02:01-positive human donors were isolated using Ficoll separation from apheresis material (AllCells, USA). Twenty-three SARS-CoV-2 peptides predicted to SaveFull Paper
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2020Lundin, V;Sugden, WW;Theodore, LN;Sousa, PM;Han, A;Chou, S;Wrighton, PJ;Cox, AG;Ingber, DE;Goessling, W;Daley, GQ;North, TE;YAP Regulates Hematopoietic Stem Cell Formation in Response to the Biomechanical Forces of Blood FlowDevelopmental cell32032546Cord Blood CD34+ Cells AllCells Cat. CB008F mPB CD34+ Cells AllCells Cat. mPB015F Mouse Embryonic Fibroblasts GlobalStem Cat. 6001G Primary Human Umbilical Vein Endothelial Cells ThermoFisher Cat. C0155CFull Paper
2020Duncan, RM;Reyes, L;Moats, K;Robinson, RM;Murphy, SA;Kaur, B;Stessman, HAF;Dolloff, NG;ATF3 Coordinates Antitumor Synergy between Epigenetic Drugs and Protein Disulfide Isomerase InhibitorsCancer research32561529T cells were expanded from human peripheral blood monoleukocytes purchased from AllCells LLC (PB006F). All cells were maintained at 37C and 5% CO 2 . Experiments were SaveFull Paper
2020Yeung, YA;Krishnamoorthy, V;Dettling, D;Sommer, C;Poulsen, K;Ni, I;Pham, A;Chen, W;Liao-Chan, S;Lindquist, K;Chin, SM;Chunyk, AG;Hu, W;Sasu, B;Chaparro-Riggers, J;Djuretic, I;An Optimized Full-Length FLT3/CD3 Bispecific Antibody Demonstrates Potent Anti-leukemia Activity and Reversible Hematological ToxicityMolecular therapy : the journal of the American Society of Gene Therapy31981494After tumor engraftment (day 6-7), animals were administered 2 × 10 7 expanded human T cells (T cells purchased from Allcells; activated according to Miltenyi manufacturing protocol) SaveFull Paper
2020Morgan, RA;Unti, MJ;Aleshe, B;Brown, D;Osborne, KS;Koziol, C;Ayoub, PG;Smith, OB;O'Brien, R;Tam, C;Miyahira, E;Ruiz, M;Quintos, JP;Senadheera, S;Hollis, RP;Kohn, DB;Improved Titer and Gene Transfer by Lentiviral Vectors Using Novel, Small β-Globin Locus Control Region ElementsMolecular therapy : the journal of the American Society of Gene Therapy31628051All BM aspirates were obtained from voluntary healthy donors supplied by AllCells (Alameda, CA), which obviated the need for Instituional Review Board review. BM mononuclear cells SaveFull Paper
2020Sinnakannu, JR;Lee, KL;Cheng, S;Li, J;Yu, M;Tan, SP;Ong, CCH;Li, H;Than, H;Anczuków-Camarda, O;Krainer, AR;Roca, X;Rozen, SG;Iqbal, J;Yang, H;Chuah, C;Ong, ST;SRSF1 mediates cytokine-induced impaired imatinib sensitivity in chronic myeloid leukemiaLeukemia32051529at the Singapore General Hospital, cord blood samples were obtained from the Singapore Cord Blood Bank, and normal bone marrow cells were obtained commercially from AllCells ( SaveFull Paper
2020Korotchkina, L;Kazyulkin, D;Komarov, PG;Polinsky, A;Andrianova, EL;Joshi, S;Gupta, M;Vujcic, S;Kononov, E;Toshkov, I;Tian, Y;Krasnov, P;Chernov, MV;Veith, J;Antoch, MP;Middlemiss, S;Somers, K;Lock, RB;Norris, MD;Henderson, MJ;Haber, M;Chernova, OB;Gudkov, AV;OT-82, a novel anticancer drug candidate that targets the strong dependence of hematological malignancies on NAD biosynthesisLeukemia31896781Normal human bone marrow mononuclear cells (BM-MNC) were obtained from ALLCELLS, LLC (Almeda CA, USA). Patients’ BM-MNC were procured at the Hematologic Bank of RPCCC and NAMPT monoclonal antibody (clone OMNI 379) was obtained from Cayman Chemicals (Full Paper
2020Kravtsova-Ivantsiv, Y;Goldhirsh, G;Ivantsiv, A;Ben Itzhak, O;Kwon, YT;Pikarsky, E;Ciechanover, A;Excess of the NF-ĸB p50 subunit generated by the ubiquitin ligase KPC1 suppresses tumors via PD-L1- and chemokines-mediated mechanismsProceedings of the National Academy of Sciences of the United States of America33168738Human PBMCs as a source for isolating human NK cells were from AllCells. Mouse anti-CD274 (PD-L1) (29E.2A3) and mouse anti-CD45 (HI30) for mass cytometry were from Bio SaveFull Paper
2020Ojaghi, A;Carrazana, G;Caruso, C;Abbas, A;Myers, DR;Lam, WA;Robles, FE;Label-free hematology analysis using deep-ultraviolet microscopyProceedings of the National Academy of Sciences of the United States of America32561645bone marrow aspirate was collected from healthy donors (AllCells) and added to Na heparin anticoagulant solution. Smears were made on uncoated quartz slides using 10 μL of whole SaveFull Paper
2020Huang, P;Zhao, Y;Zhong, J;Zhang, X;Liu, Q;Qiu, X;Chen, S;Yan, H;Hillyer, C;Mohandas, N;Pan, X;Xu, X;Putative regulators for the continuum of erythroid differentiation revealed by single-cell transcriptome of human BM and UCB cellsProceedings of the National Academy of Sciences of the United States of America32457162CD34 + stem/progenitor cells (AllCells) were obtained from three mobilized BM and three neonate UCB samples from independent donors. Cells were expanded at 37 C and 5% CO 2 SaveFull Paper
2020Belling, JN;Heidenreich, LK;Tian, Z;Mendoza, AM;Chiou, TT;Gong, Y;Chen, NY;Young, TD;Wattanatorn, N;Park, JH;Scarabelli, L;Chiang, N;Takahashi, J;Young, SG;Stieg, AZ;De Oliveira, S;Huang, TJ;Weiss, PS;Jonas, SJ;Acoustofluidic sonoporation for gene delivery to human hematopoietic stem and progenitor cellsProceedings of the National Academy of Sciences of the United States of America32358194Mixed donor umbilical cord CD34+ Hematopoietic stem and progenitor cells (Allcells Inc.) were thawed and prestimulated as described by Hoban et al. (44). Postacoustofluidic treatment, HSPCs were cultured in X-VIVO 15 supplemented with 50 ng/μL recombFull Paper
2020Borodovsky, A;Barbon, CM;Wang, Y;Ye, M;Prickett, L;Chandra, D;Shaw, J;Deng, N;Sachsenmeier, K;Clarke, JD;Linghu, B;Brown, GA;Brown, J;Congreve, M;Cheng, RK;Dore, AS;Hurrell, E;Shao, W;Woessner, R;Reimer, C;Drew, L;Fawell, S;Schuller, AG;Mele, DA;Small molecule AZD4635 inhibitor of A2AR signaling rescues immune cell function including CD103+ dendritic cells enhancing anti-tumor immunityJournal for immunotherapy of cancer3272781001 individuals. As previously described,38 naïve CD8+ T cells were co-cultured with autologous monocyte-derived DCs, both cell populations isolated from PBMC from HLA-A*0201 positive healthy donors (AllCells, Research Blood Components). Monocytes werFull Paper
2020Kotanides, H;Li, Y;Malabunga, M;Carpenito, C;Eastman, SW;Shen, Y;Wang, G;Inigo, I;Surguladze, D;Pennello, AL;Persaud, K;Hindi, S;Topper, M;Chen, X;Zhang, Y;Bulaon, DK;Bailey, T;Lao, Y;Han, B;Torgerson, S;Chin, D;Sonyi, A;Haidar, JN;Novosiadly, RD;Moxham, CM;Plowman, GD;Ludwig, DL;Kalos, M;Bispecific Targeting of PD-1 and PD-L1 Enhances T-cell Activation and Antitumor ImmunityCancer immunology research32873605Monocytes were isolated from frozen normal peripheral blood mononuclear cells (PBMC) purchased from AllCells (PB005F) using Monocyte Isolation Kit (Miltenyi Biotec; 130-091-153). Immature DCs were generated by culturing the monocytes in RPMI1640 mediFull Paper
2020Liu, Y;Lv, S;Liu, D;Song, F;Recent development of amorphous metal coordination polymers for cancer therapyActa biomaterialia32942012e) IC 50 values obtained from cytotoxicity assays against Jurkat human ALLcells after incubating with free MTX, Gd-MTX, [email protected]/DOPE, and [email protected]/DOPE-AA. f) SaveFull Paper
2020Fujita, H;Sasaki, T;Miyamoto, T;Akutsu, SN;Sato, S;Mori, T;Nakabayashi, K;Hata, K;Suzuki, H;Kosaki, K;Matsuura, S;Matsubara, Y;Amagai, M;Kubo, A;Premature aging syndrome showing random chromosome number instabilities with CDC20 mutationAging cell33094908An age-matched normal control bone marrow mononuclear cell sample was obtained from AllCells. Cells were cultured in MethoCult H4034 Optimum (STEMCELL Technologies) at 1 × SaveFull Paper
2020Yao, X;Ding, J;Effects of Microstripe Geometry on Guided Cell MigrationACS applied materials & interfaces32479054Primary rat mesenchymal stem cells (rMSCs) were purchased from Shanghai Allcells Tech. (China) and cultured in minimum essential medium α (MEM α, Gibco). Mouse embryo fibroblast cell line (NIH3T3) and human cervix epithelial carcinoma cell line (HelaFull Paper
2020Yang, H;Qing, K;Keeler, GD;Yin, L;Mietzsch, M;Ling, C;Hoffman, BE;Agbandje-McKenna, M;Tan, M;Wang, W;Srivastava, A;Enhanced Transduction of Human Hematopoietic Stem Cells by AAV6 Vectors: Implications in Gene Therapy and Genome EditingMolecular therapy. Nucleic acids32276210D, USA) supplemented with 10% fetal bovine serum (FBS; Sigma, St. Louis, MO, USA) and 1% penicillin-streptomycin (Invitrogen, Grand Island, NY, USA). Human bone marrow CD34+ cells were purchased from AllCells (AllCells Technologies, Emeryville, CA, UFull Paper
2020Shin, JJ;Schröder, MS;Caiado, F;Wyman, SK;Bray, NL;Bordi, M;Dewitt, MA;Vu, JT;Kim, WT;Hockemeyer, D;Manz, MG;Corn, JE;Controlled Cycling and Quiescence Enables Efficient HDR in Engraftment-Enriched Adult Hematopoietic Stem and Progenitor CellsCell reports32877675RNA-sequencing datasets This paper BioProject ID PRJNA498122 ------------------------- Experimental Models ------------------------- Mobilized peripheral blood CD34+ Stem/Progenitor cells AllCells mPB015F NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzFull Paper
2020Nie, W;Zan, X;Yu, T;Ran, M;Hong, Z;He, Y;Yang, T;Ju, Y;Gao, X;Synergetic therapy of glioma mediated by a dual delivery system loading α-mangostin and doxorubicin through cell cycle arrest and apoptotic pathwaysCell death & disease33116114animals The glioma cell lines C6, Gl261 and U87 and human umbilical vein endothelial cells (HUVECs) were purchased from the American Type Culture Collection (ATCC). Primary HUVECs were purchased from ALLCELLS (Shanghai, China). An EGM-2 BulletKit (LoFull Paper
2020Zhang, XY;Rajagopalan, D;Chung, TH;Hooi, L;Toh, TB;Tian, JS;Rashid, MBMA;Sahib, NRBM;Gu, M;Lim, JJ;Wang, W;Chng, WJ;Jha, S;Chow, EK;Frequent upregulation of G9a promotes RelB-dependent proliferation and survival in multiple myelomaExperimental hematology & oncology32477831e), 1% Pen Strep (Invitrogen). For adherent cell lines, trypsin (Thermo Scientific, cat# 25200-056) was used to dissociate cells each passage. Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs, AllCells) were cultured in RPMI 1640 medium (BioWeFull Paper
2020Qian, Z;Fei, J;Zong, S;Yang, K;Li, L;Liu, R;Wang, Z;Cui, Y;In Situ Visualization and SERS Monitoring of the Interaction between Tumor and Endothelial Cells Using 3D Microfluidic NetworksACS sensors31885254Human umbilical vein endothelial cells (HUVEC) were purchased from Shanghai AllCells Biotech. Co., Ltd. Breast cancer cells (MCF7) were purchased from Nanjing KeyGen Biotech. Co., Ltd. The Roswell Park Memorial Institute (RMPI) 1640 cell culture mediFull Paper
2020Kim, YH;Park, GY;Rabinovitch, N;Tarafder, S;Lee, CH;Effect of local anesthetics on viability and differentiation of various adult stem/progenitor cellsStem cell research & therapy32894184udy that directly compared the effects of LAs across various stem/progenitor cells. Materials and methods Cell isolation Human bone marrow mesenchymal stem/progenitor cells (MSCs) were obtained from AllCells (Alameda, CA). With the Institutional RevFull Paper
2020Xiong, MG;Xu, ZS;Li, YH;Wang, SY;Wang, YY;Ran, Y;RNF152 positively regulates TLR/IL-1R signaling by enhancing MyD88 oligomerizationEMBO reports31930677HEK293T cells were purchased from ATCC. Human PBMCs were purchased from ALLCELLS. HFFs provided by Prof. Min‐Hua Luo (Wuhan Institute of Virology, CAS) were derived from human foreskins (male) 30. These cells were cultured in DMEM (HyClone) supplemenFull Paper
2020Han, Y;Gong, T;Zhang, C;Dissanayaka, WL;HIF-1α Stabilization Enhances Angio-/Vasculogenic Properties of SHEDJournal of dental research32298193SHED were purchased from AllCells at passage 2 and characterized for mesenchymal origin. The results were published in our previous study (Xu et al. 2017). The cells were cultured in α-Modified Eagle’s Medium supplemented with 10% (v/v) fetal bovineFull Paper
2020Maser, IP;Hoves, S;Bayer, C;Heidkamp, G;Nimmerjahn, F;Eckmann, J;Ries, CH;The Tumor Milieu Promotes Functional Human Tumor-Resident Plasmacytoid Dendritic Cells in Humanized Mouse ModelsFrontiers in immunology33013879sulfan (Busilvex ,Pierre Faber) diluted in 0.9% Saline (Braun) was injected intraperitoneally (i.p.). Twenty-four hours later 105 human CD34+ umbilical cord blood-derived HSC (Stem Cell Technologies/Allcells) in 100 μl phosphate-buffered saline (PBS)Full Paper
2020Ghelani, A;Bates, D;Conner, K;Wu, MZ;Lu, J;Hu, YL;Li, CM;Chaudhry, A;Sohn, SJ;Defining the Threshold IL-2 Signal Required for Induction of Selective Treg Cell Responses Using Engineered IL-2 MuteinsFrontiers in immunology32582190CD8+ T cells were purified from PBMCs isolated from healthy human donor derived leukopaks (AllCells) using EasySep CD8+ T cell Negative selection kit (STEMCELL Technologies) SaveFull Paper
2020Shen, S;Sckisel, G;Sahoo, A;Lalani, A;Otter, DD;Pearson, J;DeVoss, J;Cheng, J;Casey, SC;Case, R;Yang, M;Low, R;Daris, M;Fan, B;Agrawal, NJ;Ali, K;Engineered IL-21 Cytokine Muteins Fused to Anti-PD-1 Antibodies Can Improve CD8+ T Cell Function and Anti-tumor ImmunityFrontiers in immunology32457754Mismatched donor pair leukopaks were obtained from AllCells Inc., Donor's T cells were isolated using Pan T-cell Isolation Kit (Miltenyi Biotec, # 130-096-535) and a mismatched donor' SaveFull Paper
2020Porcellini, S;Asperti, C;Corna, S;Cicoria, E;Valtolina, V;Stornaiuolo, A;Valentinis, B;Bordignon, C;Traversari, C;CAR T Cells Redirected to CD44v6 Control Tumor Growth in Lung and Ovary Adenocarcinoma Bearing MiceFrontiers in immunology32117253in of the ΔLNGFr (12). Generation of CD44v6 and CD19 CAR T Cells Peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats or leukapheresis of healthy donors (San Raffaele Hospital; AllCells) by density gradient centrifugation (LymphFull Paper
2020Baslan, T;Kendall, J;Volyanskyy, K;McNamara, K;Cox, H;D'Italia, S;Ambrosio, F;Riggs, M;Rodgers, L;Leotta, A;Song, J;Mao, Y;Wu, J;Shah, R;Gularte-Mérida, R;Chadalavada, K;Nanjangud, G;Varadan, V;Gordon, A;Curtis, C;Krasnitz, A;Dimitrova, N;Harris, L;Wigler, M;Hicks, J;Novel insights into breast cancer copy number genetic heterogeneity revealed by single-cell genome sequencingeLife32401198ta (i.e. ploidy) to achieve absolute copy number values. T-cell and B-cell sample processing and analysis Purified CD8+/CD4+ T-cells and CD19+/CD27+ memory B-cells purified cells were purchased from ALLCELLS (California, USA). Cells were thawed on iFull Paper
2020Münchow, EA;da Silva, AF;Piva, E;Cuevas-Suárez, CE;de Albuquerque, MTP;Pinal, R;Gregory, RL;Breschi, L;Bottino, MC;Development of an antibacterial and anti-metalloproteinase dental adhesive for long-lasting resin composite restorationsJournal of materials chemistry. B33169763Human dental pulp stem cells (hDPSCs, AllCells, LLC, Alameda, CA, USA) obtained from permanent third molars were cultured in low glucose Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, Invitrogen Corporation, Grand Island, NY, USA) supplemented withFull Paper
2020Warnecke, A;Harre, J;Staecker, H;Prenzler, N;Strunk, D;Couillard-Despres, S;Romanelli, P;Hollerweger, J;Lassacher, T;Auer, D;Pachler, K;Wietzorrek, G;Köhl, U;Lenarz, T;Schallmoser, K;Laner-Plamberger, S;Falk, CS;Rohde, E;Gimona, M;Extracellular vesicles from human multipotent stromal cells protect against hearing loss after noise trauma in vivoClinical and translational medicine33377658Human bone marrow (BM)-derived MSCs for the production of research-grade EV preparations were purchased from AllCells (Alameda, CA). Immunophenotype and viability analysis of SaveFull Paper
2020Pinho, AC;Fonseca, AC;Caseiro, AR;Pedrosa, SS;Amorim, I;Branquinho, MV;Domingos, M;Maurício, AC;Santos, JD;Serra, AC;Coelho, JFJ;Innovative tailor made dextran based membranes with excellent non-inflammatory response: In vivo assessmentMaterials science & engineering. C, Materials for biological applications31761159Human dental pulp stem cells (hDPSCs) were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01). Minimum Essential Medium (MEM) α, GlutaMAX™ Supplement, no nucleosides, Streptomycin, Amphotericin B and HEPES buffer solution were purchaseFull Paper
2020Wang, J;Ganaie, SS;Cheng, F;Xu, P;Ning, K;Wang, X;Kleiboeker, S;Cheng, S;Qiu, J;RNA Binding Motif Protein RBM45 Regulates Expression of the 11-Kilodalton Protein of Parvovirus B19 through Binding to Novel Intron Splicing EnhancersmBio32156816se conditions are dependent on RBM45. MATERIALS AND METHODS Ethics statement. Primary CD34+ hematopoietic stem cells were isolated from bone marrow of healthy human donors. We purchased the cells at AllCells (AllCells LLC, Alameda, CA). All of the cFull Paper
2020Dubrovsky, L;Ward, A;Choi, SH;Pushkarsky, T;Brichacek, B;Vanpouille, C;Adzhubei, AA;Mukhamedova, N;Sviridov, D;Margolis, L;Jones, RB;Miller, YI;Bukrinsky, M;Inhibition of HIV Replication by Apolipoprotein A-I Binding Protein Targeting the Lipid RaftsmBio31964734PBMCs from donors with the following HLA-B genotypes were purchased from AllCells Inc.: B*35 positive (B*15:17:01/B*35:01:01, B*35:01:01/B*55:01:01, B*35:01:01/B*35:01:01, B*35: SaveFull Paper
2020Han, KH;Arlian, BM;Lin, CW;Jin, HY;Kang, GH;Lee, S;Lee, PC;Lerner, RA;Agonist Antibody Converts Stem Cells into Migrating Brown Adipocyte-Like Cells in HeartCells31968623one, Chicago, IL, USA) and 1% penicillin and streptomycin (Invitrogen, Carlsbad, CA, USA). Expi293F cells were cultured in Expi293 Expression Media (Invitrogen, Carlsbad, CA, USA). Human CD34+ cells (AllCells, Alameda, CA, USA) were purchased and repFull Paper
2020Zimran, E;Papa, L;Djedaini, M;Patel, A;Iancu-Rubin, C;Hoffman, R;Expansion and preservation of the functional activity of adult hematopoietic stem cells cultured ex vivo with a histone deacetylase inhibitorStem cells translational medicine31950644Cryopreserved CD34+ cells from mPB or ABM from healthy adult donors were purchased from AllCells (Alameda, California) and stored in liquid nitrogen. The highly purified (90%-98% SaveFull Paper
2020George, MJ;Prabhakara, K;Toledano-Furman, NE;Gill, BS;Wade, CE;Cotton, BA;Cap, AP;Olson, SD;Cox, CS;Procoagulant in vitro effects of clinical cellular therapeutics in a severely injured trauma populationStem cells translational medicine31903737Bone marrow mononuclear cells (BM MNCs) were isolated from fresh whole bone marrow from a commercial source (AllCells, Emeryville, California) according to common protocols SaveFull Paper
2020Hou, L;Voit, RA;Sankaran, VG;Springer, TA;Yuki, K;CD11c regulates hematopoietic stem and progenitor cells under stressBlood advances33351105Human bone marrow cells and whole blood were purchased from AllCells (Quincy, MA). Lineage marker for human hematopoietic system includes: anti-hCD2 (RPA-2.10), anti-hCD3 ( SaveFull Paper
2020Mascarenhas, JO;Rampal, RK;Kosiorek, HE;Bhave, R;Hexner, E;Wang, ES;Gerds, A;Abboud, CN;Kremyanskaya, M;Berenzon, D;Odenike, O;Farnoud, N;Krishnan, A;Weinberg, RS;McGovern, E;Salama, ME;Najfeld, V;Medina-Martinez, JS;Arango Ossa, JE;Levine, MF;Zhou, Y;Sandy, L;Heaney, ML;Levine, RL;Mesa, RA;Dueck, AC;Hoffman, R;Phase 2 study of ruxolitinib and decitabine in patients with myeloproliferative neoplasm in accelerated and blast phaseBlood advances33104796Six control plasma samples (3 male, 3 female) collected from healthy donors between the ages of 30 and 50 years were procured from Allcells. Samples from 8 chronic-phase MF SaveFull Paper
2020Marion, W;Boettcher, S;Ruiz-Torres, S;Lummertz da Rocha, E;Lundin, V;Morris, V;Chou, S;Zhao, AM;Kubaczka, C;Aumais, O;Zhang, Y;Shimamura, A;Schlaeger, TM;North, TE;Ebert, BL;Wells, SI;Daley, GQ;Rowe, RG;An induced pluripotent stem cell model of Fanconi anemia reveals mechanisms of p53-driven progenitor cell differentiationBlood advances33002135Institutional Review Board at Boston Children’s Hospital, after informed consent was obtained. This study was performed in accordance with the Declaration of Helsinki. Normal healthy BM mononuclear cells were purchased from AllCellsFull Paper
2020Goldstein, RL;Goyos, A;Li, CM;Deegen, P;Bogner, P;Sternjak, A;Thomas, O;Klinger, M;Wahl, J;Friedrich, M;Rattel, B;Lamas, E;Min, X;Sudom, A;Farshbaf, M;Coxon, A;Balazs, M;Arvedson, T;AMG 701 induces cytotoxicity of multiple myeloma cells and depletes plasma cells in cynomolgus monkeysBlood advances32886754For staining of human PCs, human BM (AllCells) was stained with a fluorochrome-labeled antibody cocktail (supplemental Table 5). For analysis, doublets were gated out, and dead SaveFull Paper
2020Walker, ZJ;VanWyngarden, MJ;Stevens, BM;Abbott, D;Hammes, A;Langouët-Astrie, C;Smith, CA;Palmer, BE;Forsberg, PA;Mark, TM;Jordan, CT;Sherbenou, DW;Measurement of ex vivo resistance to proteasome inhibitors, IMiDs, and daratumumab during multiple myeloma progressionBlood advances32311014Normal donor samples were purchased from AllCells. For viability comparison with MM cells in MNC cultures, selection for CD138-positive cells was performed by using magnetic bead SaveFull Paper
2020Romero-Masters, JC;Huebner, SM;Ohashi, M;Bristol, JA;Benner, BE;Barlow, EA;Turk, GL;Nelson, SE;Baiu, DC;Van Sciver, N;Ranheim, EA;Gumperz, J;Sherer, NM;Farrell, PJ;Johannsen, EC;Kenney, SC;B cells infected with Type 2 Epstein-Barr virus (EBV) have increased NFATc1/NFATc2 activity and enhanced lytic gene expression in comparison to Type 1 EBV infectionPLoS pathogens32059024ized NSG mice Immunodeficient NSG (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson labs (catalogue number: 005557). Commercially available CD34-depleted human cord blood mononuclear cells (AllCells, LLC) were infected with various amounts oFull Paper
2020Shao, S;Qin, T;Qian, W;Li, X;Li, W;Han, L;Zhang, D;Wang, Z;Ma, Q;Wu, Z;Wu, E;Lei, J;Cav-1 Ablation in Pancreatic Stellate Cells Promotes Pancreatic Cancer Growth through Nrf2-Induced shh SignalingOxidative medicine and cellular longevity32377291iated Hospital of Xi'an Jiaotong University, China. 2.3. Cell Lines Aspc-1 cells were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China), and HUVECs were obtained from ALLCELLS (Emeryville, California, USA). In our expFull Paper
2020Liu, H;Cheng, Q;Xu, DS;Wang, W;Fang, Z;Xue, DD;Zheng, Y;Chang, AH;Lei, YJ;Overexpression of CXCR7 accelerates tumor growth and metastasis of lung cancer cellsRespiratory research33129326Human umbilical vein endothelial cells (HUVECs) and its completed medium were obtained from AllCells biological technology (Shanghai) co., LTD. And all these cell lines were SaveFull Paper
2020Cheng, B;Ren, Y;Cao, H;Chen, J;Discovery of novel resorcinol diphenyl ether-based PROTAC-like molecules as dual inhibitors and degraders of PD-L1European journal of medicinal chemistry32388281CD3 single chain variable fragment) and human PD-L1 (hPD-L1). Fresh PBMCs were purchased from ALLCELLS. Centrifuge at 350g for 10 min, discard the supernatant and resuspend in EasySep buffer at a density of 5 x107/mL. CD3+ T cells were isolated fromFull Paper
2020Zhang, D;Qi, BY;Zhu, WW;Huang, X;Wang, XZ;Crocin alleviates lipopolysaccharide-induced acute respiratory distress syndrome by protecting against glycocalyx damage and suppressing inflammatory signaling pathwaysInflammation research : official journal of the European Histamine Research Society ... [et al.]31925528HUVECs were cultured in complete culture medium (AllCells, Shanghai, China) in an incubator with a humidified atmosphere of 95% air and 5% CO 2 at 37 C. Cells at 80-90% SaveFull Paper
2020Kuang, Z;Jing, H;Wu, Z;Wang, J;Li, Y;Ni, H;Zhang, P;Wu, W;Wu, M;Zhou, S;Qiu, X;Wu, D;Prinz, B;Baruah, H;Chen, B;Yu, M;Liu, J;Development and characterization of a novel anti-OX40 antibody for potent immune activationCancer immunology, immunotherapy : CII32078015Two million LoVo cells suspended in 0.2 ml PBS were mixed with 0.66 million human peripheral blood mononuclear cells (PBMCs, ALLCELLS) and co-implanted subcutaneously in the SaveFull Paper
2020Yao, Y;Yang, J;Qin, Q;Tang, C;Li, Z;Chen, L;Li, K;Ren, C;Chen, L;Rao, S;Functional annotation of genetic associations by transcriptome-wide association analysis provides insights into neutrophil development regulationCommunications biology33340029Human CD34 + HSPCs from mobilized peripheral blood of deidentified healthy donors were purchased from AllCells. CD34 + HSPCs were cultured in StemSpan SFEM medium ( SaveFull Paper
2020Mendes, LP;Rostamizadeh, K;Gollomp, K;Myerson, JW;Marcos-Contreras, OA;Zamora, M;Luther, E;Brenner, JS;Filipczak, N;Li, X;Torchilin, VP;Monoclonal antibody 2C5 specifically targets neutrophil extracellular trapsmAbs33323006Whole blood was purchased from AllCells (AllCells utilizes standardized operating HIPAA compliance and approved protocols are followed by AllCells' collection facilities. The staff SaveFull Paper
2020Zang, L;Song, Y;Yu, F;Liu, X;Emodin relieved lipopolysaccharide-evoked inflammatory damage in WI-38 cells by up-regulating taurine up-regulated gene 1BioFactors (Oxford, England)31912578The human fibroblasts cell line WI-38 (AllCells, Shanghai, China) was hatched in a mixture of Dulbecco's modified Eagle's medium (DMEM; Gibco, Grand Island, NY), endothelial cell development supplement (Sigma, St. Louis, MO) (60 μg/ml), streptomycinFull Paper
2020Subhi, H;Husein, A;Mohamad, D;Nurul, A;Physicochemical, mechanical and cytotoxicity evaluation of chitosan-based accelerated portland cementJournal of Materials Research and TechnologyStem cells from human exfoliated deciduous teeth (SHED) was purchased from AllCells, USA. The cells were cultured in alpha minimum essential medium [Invitrogen, USA (GIBCO)] SaveFull Paper
2020Hu, S;Cavanagh, BL;Harrington, R;Ahmad, M;Kearns, G;Meaney, S;Wynne, C;A Novel Pool of Microparticle Cholesterol Is Elevated in Rheumatoid Arthritis but Not in Systemic Lupus Erythematosus PatientsInternational journal of molecular sciences33287382Microparticles are sub-micron, membrane-bound particles released from virtually allcells and which are present in the circulation. In several autoimmune disorders their amountand SaveFull Paper
2020Wen, W;Song, S;Han, Y;Chen, H;Liu, X;Qian, Q;An efficient Screening System in Yeast to Select a Hyperactive piggyBac Transposase for Mammalian ApplicationsInternational journal of molecular sciences32357554fied eagle medium (DMEM; Corning, Corning, NY, USA) supplemented with 10% fetal calf serum (FBS, Gibco, USA). The human peripheral blood mononuclear cells (PBMC) of healthy donors were purchased from AllCells (AllCells, Silicon Valley, CA, USA) underFull Paper
2020Damodharan, SN;Walker, KL;Forsberg, MH;McDowell, KA;Bouchlaka, MN;Drier, DA;Sondel, PM;DeSantes, KB;Capitini, CM;Analysis of ex vivo expanded and activated clinical-grade human NK cells after cryopreservationCytotherapy32536506All cellular expansions occurred at the WBF under current Good Manufacturing Practice (GMP) quality system guidelines. Fresh leukopacks were commercially obtained (AllCells, Alameda, CA, USA, and Key Biologics, Memphis, TN, USA) from paid human apherFull Paper
2020Chen, Q;Jia, G;Zhao, X;Bao, Y;Zhang, Y;Ozkan, C;Minev, B;Ma, W;Novel Survivin Peptides Screened With Computer Algorithm Induce Cytotoxic T Lymphocytes With Higher Cytotoxic Efficiency to Cancer CellsFrontiers in molecular biosciences33102521PBMCs from five healthy donors were provided by San Diego Blood Bank and Scripps Green Hospital (San Diego, CA, United States). Normal human umbilical cord blood (UCB) CD34+ cells were purchased from AllCells (Alameda, CA, United States). This studyFull Paper
2020Papa, L;Djedaini, M;Martin, TC;Zangui, M;Beaumont, KG;Sebra, R;Parsons, R;Schaniel, C;Hoffman, R;Limited Mitochondrial Activity Coupled With Strong Expression of CD34, CD90 and EPCR Determines the Functional Fitness of ex vivo Expanded Human Hematopoietic Stem CellsFrontiers in cell and developmental biology33384995Cryopreserved CD34 + cells from G-CSF mobilized peripheral blood (PB) or bone marrow (BM) aspirates harvested from healthy adult donors were purchased from AllCells. SaveFull Paper
2020Yao, X;Xie, L;Zeng, Y;MiR-9 Promotes Angiogenesis via Targeting on Sphingosine-1- Phosphate Receptor 1Frontiers in cell and developmental biology32850858and S1P3 in those normal ECs and tumor ECs were compared. The relationships between the significant differentially expressed miR-9 loci and specific S1P receptors were analyzed. Cell Culture HUVECs (Allcells, Shanghai, China) were cultured in HUVECFull Paper
2020Juillerat, A;Tkach, D;Yang, M;Boyne, A;Valton, J;Poirot, L;Duchateau, P;Straightforward Generation of Ultrapure Off-the-Shelf Allogeneic CAR-T CellsFrontiers in bioengineering and biotechnology32671047ndependent T-cell donors. Significance is determined by a standard unpaired t-test, *p ≤ 0.05, **p ≤ 0.01. Materials and Methods T-Cell Proliferation Cryopreserved human PBMCs were acquired from ALLCELLS (cat #PB006F) and used in accordance with CelFull Paper
2020Kuang, Z;Pu, P;Wu, M;Wu, Z;Wang, L;Li, Y;Zhang, S;Jing, H;Wu, W;Chen, B;Liu, J;A Novel Bispecific Antibody with PD-L1-assisted OX40 Activation for Cancer TreatmentMolecular cancer therapeutics32999045Monocyte-derived immature dendrite cells (DC) isolated from human PBMCs (ALLCELLS) were maintained in AIM V medium CTS medium supplemented with GM-CSF (10 ng/mL, R&D SaveFull Paper
2020Kotanides, H;Sattler, RM;Lebron, MB;Carpenito, C;Shen, J;Li, J;Surguladze, D;Haidar, JN;Burns, C;Shen, L;Inigo, I;Pennello, AL;Forest, A;Chen, X;Chin, D;Sonyi, A;Topper, M;Boucher, L;Sharma, P;Zhang, Y;Burtrum, D;Novosiadly, RD;Ludwig, DL;Plowman, GD;Kalos, M;Characterization of 7A5: A Human CD137 (4-1BB) Receptor Binding Monoclonal Antibody with Differential Agonist Properties That Promotes Antitumor ImmunityMolecular cancer therapeutics32241872Human PBMCs were isolated from whole blood or leukopak obtained from NY Blood Center, AllCells, or Biological Specialty using Ficoll density gradient centrifugation (Ficoll-Paque SaveFull Paper
2020Bian, W;Jing, X;Yang, Z;Shi, Z;Chen, R;Xu, A;Wang, N;Jiang, J;Yang, C;Zhang, D;Li, L;Wang, H;Wang, J;Sun, Y;Zhang, C;Downregulation of LncRNA NORAD promotes Ox-LDL-induced vascular endothelial cell injury and atherosclerosisAging32267831(Shanghai, China). The C57BL/6 J mice fed with a normal diet were labeled as the WT control group. Cell culture, RNA interference and Adenovirus infection Primary HUVECs were obtained from Shanghai AllCells Biotech Co., Ltd. (Shanghai, China) and cuFull Paper
2020Karuna, R;Yokokawa, F;Wang, K;Zhang, J;Xu, H;Wang, G;Ding, M;Chan, WL;Abdul Ghafar, N;Leonardi, A;Seh, CC;Seah, PG;Liu, W;Srinivasa, RPS;Lim, SP;Lakshminarayana, SB;Growcott, E;Babu, S;Fenaux, M;Zhong, W;Gu, F;Shi, PY;Blasco, F;Chen, YL;A Cyclic Phosphoramidate Prodrug of 2'-Deoxy-2'-Fluoro-2'-C-Methylguanosine for the Treatment of Dengue Virus InfectionAntimicrobial agents and chemotherapy32958712PBMCs (individual donors) were purchased from AllCells (Alameda, CA) or ReachBio (Seattle, WA). Written consent from the donors was available for all samples. All experiments involving human matrices were approved by the Institutional Review Board ofFull Paper
2020Sebrow, J;Goff, SP;Griffin, DO;Successfully transfected primary peripherally mobilized human CD34+ hematopoietic stem and progenitor cells (HSPCs) demonstrate increased susceptibility to retroviral infectionVirology journal32039735We obtained 293 T cells and Jurkat cells from ATCC, and peripherally mobilized CD34+ HSPCs from individual donors from AllCells (pre-isolated). 293 T cells were cultured in 20 mm X SaveFull Paper
2020Zhong, C;Yu, Q;Jia, W;Yu, X;Yu, D;Yang, M;Wang, L;Ling, C;Zhu, L;Mechanism for enhanced transduction of hematopoietic cells by recombinant adeno-associated virus serotype 6 vectorsFASEB journal : official publication of the Federation of American Societies for Experimental Biology32960474Primary human cord blood-derived CD34 + hematopoietic progenitors (mixed, 80% purity) were purchased from AllCells (Alameda, CA, USA) and cultured in C-IMDM in the presence of 1 ng/mL of recombinant human stem cell factor (rhSCF, BD Bioscience, San DFull Paper
2020Li, L;Wu, Z;Wu, M;Qiu, X;Wu, Y;Kuang, Z;Wang, L;Sun, T;Liu, Y;Yi, S;Jing, H;Zhou, S;Chen, B;Wu, D;Wu, W;Liu, J;IBI112, a selective anti-IL23p19 monoclonal antibody, displays high efficacy in IL-23-induced psoriasiform dermatitisInternational immunopharmacology33069927Mouse (C57BL/6, Beijing Vital River Laboratory Animal Technology) or cynomolgus monkey splenocytes (AllCells, USA) were cultured in the presence of IL-2 (R&D, USA). Different SaveFull Paper
2020Kaneko, Y;Fukahori, H;Yamagami, K;Kawashima, T;Ito, M;Akamatsu, M;Marui, T;Kato, K;Takahashi, F;Morokata, T;Effects of AS2819899, a novel selective PI3Kδ inhibitor, in a NZB/W F1 mouse lupus-like nephritis modelInternational immunopharmacology32736191Peripheral blood-derived frozen normal pan-T cells (AllCells, Alameda, CA, USA) were thawed according to the manufacturer’s instructions. The cell suspension (1 × 105 cells/well) was mixed with AS2819899 at designated concentrations in the anti-CD3 aFull Paper
2020Goydel, RS;Weber, J;Peng, H;Qi, J;Soden, J;Freeth, J;Park, H;Rader, C;Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applicationsThe Journal of biological chemistry32193207Thr-245 and Ala-245, which arise from an SNP (rs10820900) in the frizzled domain of human ROR2 (hROR2-Fz). Human PBMCs were purchased from AllCells and cultured in X-VIVO 20 medium (Lonza) with 5% (v/v) off-the-clot human AB serum (Gemini Bio-ProductFull Paper
2020Čuperlović-Culf, M;Khieu, NH;Surendra, A;Hewitt, M;Charlebois, C;Sandhu, JK;Analysis and Simulation of Glioblastoma Cell Lines-Derived Extracellular Vesicles MetabolomeMetabolites32131411Normal human astrocytes (NHA, Lonza, Walkersville, MD, USA) were used as normal counterparts and were grown in astrocyte medium-containing supplements (Allcells) and 5% FBS. SaveFull Paper
2020Liu, J;Kozhaya, L;Torres, VJ;Unutmaz, D;Lu, M;Structure-based discovery of a small-molecule inhibitor of methicillin-resistant Staphylococcus aureus virulenceThe Journal of biological chemistry32179646Blood samples were obtained from healthy, consenting donors as buffy coats (New York Blood Center) and leukopaks (AllCells, Alameda, CA). Human PBMCs were isolated from peripheral blood by density gradient centrifugation using Ficoll-Paque Plus (GE LFull Paper
2020Thwin, KKM;Ishida, T;Uemura, S;Yamamoto, N;Lin, KS;Tamura, A;Kozaki, A;Saito, A;Kishimoto, K;Mori, T;Hasegawa, D;Kosaka, Y;Nino, N;Takafuji, S;Iijima, K;Nishimura, N;Level of Seven Neuroblastoma-Associated mRNAs Detected by Droplet Digital PCR Is Associated with Tumor Relapse/Regrowth of High-Risk Neuroblastoma PatientsThe Journal of molecular diagnostics : JMD318374271 sample from BioChain (Newark, CA), 2 samples from STEMCELL (Vancouver, Canada), 56 samples from AllCells (Alameda, CA), 16 samples from ReachBio (Seattle, WA), and 28 samples from Loza (Walkersville, MD) (Supplemental Table S1). All PB samples fromFull Paper
2020Xue, WL;Chen, RQ;Zhang, QQ;Li, XH;Cao, L;Li, MY;Li, Y;Lin, G;Chen, Y;Wang, MJ;Zhu, YC;Hydrogen sulfide rescues high glucose-induced migration dysfunction in HUVECs by upregulating miR-126-3pAmerican journal of physiology. Cell physiology32186933Primary human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) (35). These HUVECs were cultured in endothelial complete medium (Allcells, SaveFull Paper
2020Satani, N;Giridhar, K;Cai, C;Wewior, N;Norris, DD;Aronowski, J;Savitz, SI;Medications for Hypertension Change the Secretome Profile from Marrow Stromal Cells and Peripheral Blood MonocytesStem cells international32802081MSCs were isolated from commercially available fresh human bone marrow aspirates (AllCells, Alameda, CA) using density centrifugation and plastic adherence as previously SaveFull Paper
2020Diaz, MF;Horton, PD;Dumbali, SP;Kumar, A;Livingston, M;Skibber, MA;Mohammadalipour, A;Gill, BS;Zhang, S;Cox, CS;Wenzel, PL;Bone marrow stromal cell therapy improves survival after radiation injury but does not restore endogenous hematopoiesisScientific reports33335275t received wet feed on days 4-30 post-exposure. Culture of human MSCs Human MSCs used for cell therapy were derived from whole bone marrow from independent human donors (commercially obtained from AllCells, LLC, Alameda, CA). Cells were isolated andFull Paper
2020Dzimianski, JV;Lorig-Roach, N;O'Rourke, SM;Alexander, DL;Kimmey, JM;DuBois, RM;Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometryScientific reports33303951dy at UCSC. All participants agreed to sample banking and future research use. The convalescent seropositive (SP) panel comprised 10 de-identified plasma samples from nine individuals, purchased from AllCells (Alameda, CA, USA). To be eligible for plFull Paper
2020Diaz, MF;Horton, PD;Kumar, A;Livingston, M;Mohammadalipour, A;Xue, H;Skibber, MA;Ewere, A;Toledano Furman, NE;Aroom, KR;Zhang, S;Gill, BS;Cox, CS;Wenzel, PL;Injury intensifies T cell mediated graft-versus-host disease in a humanized model of traumatic brain injuryScientific reports32612177Bone marrow stromal cells were derived from whole bone marrow from independent human donors (AllCells, Alameda, CA). Mononuclear cells from whole bone marrow were enriched SaveFull Paper
2020Allen, A;Vaninov, N;Li, M;Nguyen, S;Singh, M;Igo, P;Tilles, AW;O'Rourke, B;Miller, BLK;Parekkadan, B;Barcia, RN;Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune CellsScientific reports32576889ld times cover the range of expected delivery of the device from cell seeding location to clinical application. PBMC isolation and enrichment Peripheral blood mononuclear cells (PBMCs) obtained from AllCells LLC (AllCells, CA, USA) were freshly isolFull Paper
2020Siew Ching, H;Thirumulu Ponnuraj, K;Luddin, N;Ab Rahman, I;Nik Abdul Ghani, NR;Early Odontogenic Differentiation of Dental Pulp Stem Cells Treated with Nanohydroxyapatite-Silica-Glass Ionomer CementPolymers32957636the present study was principally aimed at evaluating the effect of nanoHA-silica-GIC on the differentiation of DPSCs into odontogenic lineage. 2. Materials and Methods 2.1. Cell Culture DPSCs (AllCells, Alameda, CA, USA; Cat no. DP003F) were grownFull Paper
2020Keskar, V;Sood, A;Loghin, E;Kovacs, E;Duthie, RS;Liu, S;Park, JH;Chadwick, C;Smith, R;Brown, M;Stroncek, DF;Highfill, SL;Novel DNA-based T-Cell Activator Promotes Rapid T-Cell Activation and ExpansionJournal of immunotherapy (Hagerstown, Md. : 1997)32796275Activation studies were conducted in 6-well tissue culture plastic plates using commercially available Pan T cells (AllCells, Alameda, CA). Cultures were diluted as needed (1:3 or 1:4) to SaveFull Paper
2020Shrestha, B;Zhang, Y;Yu, B;Li, G;Boucher, JC;Beatty, NJ;Tsai, HC;Wang, X;Mishra, A;Sweet, K;Lancet, JE;Kelley, L;Davila, ML;Generation of Antitumor T Cells For Adoptive Cell Therapy With Artificial Antigen Presenting CellsJournal of immunotherapy (Hagerstown, Md. : 1997)31834208To investigate the ability of aAPCs to support T-cell expansion we isolated T cells from healthy donor PBMCs (ALLcells) using a Human T-cell isolation Kit (Stem Cells Inc., Cambridge, SaveFull Paper
2020Park, LM;Lannigan, J;Jaimes, MC;OMIP-069: Forty-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral BloodCytometry. Part A : the journal of the International Society for Analytical Cytology32830910All human PBMCs used in this study were obtained from AllCells Alameda. Ethical review and regulatory compliance were conducted by Alpha Independent Review Board under SaveFull Paper
2020Nair, RR;Piktel, D;Hathaway, QA;Rellick, SL;Thomas, P;Saralkar, P;Martin, KH;Geldenhuys, WJ;Hollander, JM;Gibson, LF;Pyrvinium Pamoate Use in a B cell Acute Lymphoblastic Leukemia Model of the Bone Tumor MicroenvironmentPharmaceutical research31989336Primary CD3+ T cells, peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) were purchased from AllCells (Allcells.com) and maintained in Lymphocyte Growth Medium-3 (Lonza, Cat No: CC-3211) containing 10% FBS and 1x strepFull Paper
2020Powell, AB;Ren, Y;Korom, M;Saunders, D;Hanley, PJ;Goldstein, H;Nixon, DF;Bollard, CM;Lynch, RM;Jones, RB;Cruz, CRY;Engineered Antigen-Specific T Cells Secreting Broadly Neutralizing Antibodies: Combining Innate and Adaptive Immune Response against HIVMolecular therapy. Methods & clinical development33005704Peripheral blood samples were obtained from deidentified, healthy donor buffy coats from the NIH, through the Department of Transfusion Medicine, or commercially from AllCells. Save;erved. Peripheral Blood Samples Peripheral blood samples were obtFull Paper
2020Fang, Y;Zhang, Y;Guo, C;Chen, C;Gao, H;Zhou, X;Liu, T;Qian, Q;Safety and Efficacy of an Immune Cell-Specific Chimeric Promoter in Regulating Anti-PD-1 Antibody Expression in CAR T CellsMolecular therapy. Methods & clinical development32995356PBMCs were purchased from AllCells (USA) using a protocol approved by the Ethics Committee of the Chinese Peoples' Liberation Army General Hospital (S2019-063-01). In total, 5 × Save;Cell Isolation, Electroporation, and Ex Vivo CAR T Cell ExpansionFull Paper
2020Shapiro, J;Iancu, O;Jacobi, AM;McNeill, MS;Turk, R;Rettig, GR;Amit, I;Tovin-Recht, A;Yakhini, Z;Behlke, MA;Hendel, A;Increasing CRISPR Efficiency and Measuring Its Specificity in HSPCs Using a Clinically Relevant SystemMolecular therapy. Methods & clinical development32478125(5 μL total volume). Complexes were allowed to form for 10-20 min at room temperature before electroporation. Genome Editing by RNP Electroporation in CD34+ HSPCs Mobilized human CD34+ HSPCs (AllCells, Alameda, CA, USA) were thawed and cultured forFull Paper
2020Morgan, RA;Ma, F;Unti, MJ;Brown, D;Ayoub, PG;Tam, C;Lathrop, L;Aleshe, B;Kurita, R;Nakamura, Y;Senadheera, S;Wong, RL;Hollis, RP;Pellegrini, M;Kohn, DB;Creating New β-Globin-Expressing Lentiviral Vectors by High-Resolution Mapping of Locus Control Region Enhancer SequencesMolecular therapy. Methods & clinical development32426415BM CD34+ Cell Culture and Transduction All BM aspirates were obtained from voluntary healthy donors supplied by AllCells (Alameda, CA, USA). BM mononuclear cells were isolated SaveFull Paper
2020Mehler, VJ;Burns, CJ;Stauss, H;Francis, RJ;Moore, ML;Human iPSC-Derived Neural Crest Stem Cells Exhibit Low ImmunogenicityMolecular therapy. Methods & clinical development32055644utamine, 2 mM L-glutamine, 50 U/mL penicillin/streptomycin, 20 ng/mL granulocyte-macrophage colony-stimulating factor (GM-CSF), and 20 ng/mL IL-4. BM-MSCs were derived from bone marrow aspirates (AllCells, Alameda, CA, USA) in-house as previously desFull Paper
2020Elnaggar, M;Al-Mohannadi, A;Kizhakayil, D;Raynaud, CM;Al-Mannai, S;Gentilcore, G;Pavlovski, I;Sathappan, A;Van Panhuys, N;Borsotti, C;Follenzi, A;Grivel, JC;Deola, S;Flow-Cytometry Platform for Intracellular Detection of FVIII in Blood Cells: A New Tool to Assess Gene Therapy Efficiency for Hemophilia AMolecular therapy. Methods & clinical development31886317ar endothelial cells) and U937 (pre-monocytic cells) cell lines were kindly provided by Antonia Follenzi (UPO, Italy). HeLa (cervical cancer cells) were purchased from ATCC. PBMCs were purchased from AllCells (Alameda, CA, USA). Cells were cultured iFull Paper
2020Ning, K;Wang, MJ;Lin, G;Zhang, YL;Li, MY;Yang, BF;Chen, Y;Huang, Y;Li, ZM;Huang, YJ;Zhu, L;Liang, K;Yu, B;Zhu, YZ;Zhu, YC;eNOS-Nitric Oxide System Contributes to a Novel Antiatherogenic Effect of Leonurine via Inflammation Inhibition and Plaque StabilizationThe Journal of pharmacology and experimental therapeutics32238453Primary human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (H-001F-C; China) and cultured in HUVEC Growth Medium (H-004; Allcells), and 100 U/ml of SaveFull Paper
2020Zhaorigetu, S;Bair, H;Jin, D;Gupta, VS;Pandit, LM;Bryan, RM;Lally, KP;Olson, SD;Cox, CS;Harting, MT;Extracellular Vesicles Attenuate Nitrofen-Mediated Human Pulmonary Artery Endothelial Dysfunction: Implications for Congenital Diaphragmatic HerniaStem cells and development32475301Human MSCs were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using density centrifugation and plastic SaveFull Paper
2020Baumgartner, JM;Riviere, P;Lanman, RB;Kelly, KJ;Veerapong, J;Lowy, AM;Kurzrock, R;Prognostic Utility of Pre- and Postoperative Circulating Tumor DNA Liquid Biopsies in Patients with Peritoneal MetastasesAnnals of surgical oncology32767050Background Circulating tumor DNA (ctDNA) is a promising technology for treatment selection, prognostication, and surveillance after definitive therapy. Its use in the perioperative SaveFull Paper
2020Hamburger, AE;DiAndreth, B;Cui, J;Daris, ME;Munguia, ML;Deshmukh, K;Mock, JY;Asuelime, GE;Lim, ED;Kreke, MR;Tokatlian, T;Kamb, A;Engineered T cells directed at tumors with defined allelic lossMolecular immunology33012527Leukopaks were purchased from AllCells®. Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB), with strict oversight. HIPAA compliance and approved protocols were also followed. Frozen PBMCs were thawedFull Paper
2020Xu, H;Hamburger, AE;Mock, JY;Wang, X;Martin, AD;Tokatlian, T;Oh, J;Daris, ME;Negri, KR;Gabrelow, GB;Wu, ML;Nampe, DP;Asuelime, GE;McElvain, ME;Sandberg, ML;Kamb, A;Structure-function relationships of chimeric antigen receptors in acute T cell responses to antigenMolecular immunology32768859Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols SaveFull Paper
2020Wang, Z;Zhou, G;Risu, N;Fu, J;Zou, Y;Tang, J;Li, L;Liu, H;Liu, Q;Zhu, X;Lenalidomide Enhances CAR-T Cell Activity Against Solid Tumor CellsCell transplantation32967454line U251 CD133-OE luc. Similarly, the tumor cell line U251 WT luc, MDA-MB-453 luc, and MDA-MB-468 luc were also obtained by nucleofection. Peripheral blood mononuclear cells (PBMCs) were bought from ALLCELLS (PB005F, Alameda, CA, USA). Following theFull Paper
2020Zhao, C;Zhao, Z;Wang, Z;Hu, L;Wang, H;Fang, Z;Supervillin promotes tumor angiogenesis in liver cancerOncology reports32468064Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells LLC (cat. H-004; AllCells LLC) in a 0.25% gelatin-coated culture flask. HepG2, Bel7405 and MHCC- SaveFull Paper
2020Lee, KS;Lee, J;Lee, P;Kim, CU;Kim, DJ;Jeong, YJ;Park, YJ;Tesh, VL;Lee, MS;Exosomes released from Shiga toxin 2a-treated human macrophages modulate inflammatory responses and induce cell death in toxin receptor expressing human cellsCellular microbiology32772454Human CD14 + monocytes were purchased from AllCells LLC and maintained in RPMI 1640 medium containing 10% FBS, 5.0 μg/ml streptomycin and 5.0 U/ml penicillin. Cells (1.0 × SaveFull Paper
2020Monroe, MN;Zhaorigetu, S;Gupta, VS;Jin, D;Givan, KD;Curylo, AL;Olson, SD;Cox, CS;Segura, A;Buja, LM;Grande-Allen, KJ;Harting, MT;Extracellular vesicles influence the pulmonary arterial extracellular matrix in congenital diaphragmatic herniaPediatric pulmonology32568428Human mesenchymal stromal cells (hMSCs) were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using SaveFull Paper
2020Zaw, SYM;Kaneko, T;Zaw, ZCT;Sone, PP;Murano, H;Gu, B;Okada, Y;Han, P;Katsube, KI;Okiji, T;Crosstalk between dental pulp stem cells and endothelial cells augments angiogenic factor expressionOral diseases32248596Stem cells from human exfoliated deciduous teeth (SHEDs; AllCells) were cultured with a stem cell growth medium (Dulbecco's modified Eagle's medium (DMEM)/F12; Gibco) containing 15% fetal bovine serum (Gibco-BRL, Grand Island, NY), 10,000 units penicFull Paper
2020Huang, GL;Nampe, DP;Yi, J;Gabrelow, GB;Negri, KR;Kamb, A;Xu, H;A multivariate, quantitative assay that disentangles key kinetic parameters of primary human T cell function in vitroPloS one33166305Collection protocols and donor informed consent were approved by an Institutional Review Board (IRB) at Allcells . Allcells also followed HIPAA compliance and approved protocols SaveFull Paper
2020Wang, X;Chacon, LI;Derakhshandeh, R;Rodriguez, HJ;Han, DD;Kostyushev, DS;Henry, TD;Traverse, JH;Moyé, L;Simari, RD;Taylor, DA;Springer, ML;Impaired therapeutic efficacy of bone marrow cells from post-myocardial infarction patients in the TIME and LateTIME clinical trialsPloS one32841277Healthy de-identified donor BM MNCs were purchased from AllCells (Alameda, CA). These MNCs were manually isolated at AllCells using Ficoll conditions like that in the Sepax SaveFull Paper
2020Schönherz, AA;Bødker, JS;Schmitz, A;Brøndum, RF;Jakobsen, LH;Roug, AS;Severinsen, MT;El-Galaly, TC;Jensen, P;Johnsen, HE;Bøgsted, M;Dybkær, K;Normal myeloid progenitor cell subset-associated gene signatures for acute myeloid leukaemia subtyping with prognostic impactPloS one32324791[18] GSE42519, [19] GSE19599, [20] GSE17054, [21] and GSE19429 [22] cohorts. For GSE63270, [18] and GSE17054, [21] human bone marrow mononuclear cells (BMMCs) from healthy donors were purchased from ALLCELLS (Emeryville, CA) where collection protocolFull Paper
2020Tani, J;Ito, Y;Tatemichi, S;Yamakami, M;Fukui, T;Hatano, Y;Kakimoto, S;Kotani, A;Sugimura, A;Mihara, K;Yamamoto, R;Tanaka, N;Minami, K;Takahashi, K;Hirato, T;Physicochemical and biological evaluation of JR-131 as a biosimilar to a long-acting erythropoiesis-stimulating agent darbepoetin alfaPloS one32302352fects of the test substances on colony-forming unit-erythroid (CFU-E)- and burst-forming unit-erythroid (BFU-E)-derived colony formation in human bone-marrow-derived mononuclear cells (purchased from AllCells, CA) were examined. The cells were obtainFull Paper
2020Steinberg, L;The Effects of P. Gingivalis Ceramide Lipids on Platelet Size, Agglutination, Aggregation, and Vascular Surface Adhesion MarkersThesisBackground: Increased bleeding on probing has been proven to be correlated with the progression of periodontal disease. Gingival bleeding in sites with periodontitis has often been attributed to chronic inflammation. Previous research has documentedFull Paper
2020Kim, DH;Kim, DH;Heck, BE;Shaffer, M;Yoo, KH;Hur, J;PPAR-δ agonist affects adipo-chondrogenic differentiation of human mesenchymal stem cells through the expression of PPAR-γRegenerative therapy33426208In this experimental study, frozen human bone marrow mononuclear cells were used for isolation (AllCells, Alameda, CA, USA) [25]. Cells were resuspended in α-minimum essential Save;mation condition and its correlation with PPAR-δ. 2 Methods 2.1 IsFull Paper
2020Zhang, E;Xie, L;Qin, P;Lu, L;Xu, Y;Gao, W;Wang, L;Xie, MH;Jiang, W;Liu, S;Quality by Design-Based Assessment for Analytical Similarity of Adalimumab Biosimilar HLX03 to Humira®The AAPS journal32385732PMBC cells (Allcells) were incubated with mAbs with a 2-fold serial dilution for 30 min followed by TNFα-Biotin (Sinobio) incubation. After labeled by Streptavidin-PE (eBioscience), SaveFull Paper
2020Dai, R;Heid, B;Xu, X;Xie, H;Reilly, CM;Ahmed, SA;EGR2 is elevated and positively regulates inflammatory IFNγ production in lupus CD4+ T cellsBMC immunology32646370ethods Human peripheral blood mononuclear cells (PBMCs) The PBMCs of human patients of lupus (n = 4, all female) and healthy controls (n = 4, 2 male and 2 female) were purchased directly from AllCells LLC (Alameda, CA, USA). Based on the informationFull Paper
2020Dong, W;Zhang, X;Lu, Z;Effect of 532nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor in cultured human vascular endothelial cellsPhotodiagnosis and photodynamic therapy32344194Working drug solutions (0-8 μg/ml) were prepared by diluting the stock solution with RPMI-1640 medium (AllCells). A 532 nm neodymium-doped yttrium orthovanadate (Nd:YVO 4 ) SaveFull Paper
2020Pieles, O;Reck, A;Reichert, TE;Morsczeck, C;p53 inhibits the osteogenic differentiation but does not induce senescence in human dental follicle cellsDifferentiation; research in biological diversity32473528Human dental follicle cells (DFCs) were obtained from AllCells. DFCs were cultivated as described previously (Morsczeck et al., 2016). Cells were analyzed before and after the SaveFull Paper
2020Lafleur, MA;Werner, J;Fort, M;Lobenhofer, EK;Balazs, M;Goyos, A;MRGPRX2 activation as a rapid, high-throughput mechanistic-based approach for detecting peptide-mediated human mast cell degranulation liabilitiesJournal of immunotoxicology32525431Human mobilized peripheral blood CD34+ cells (derived from healthy donors treated with G-CSF to mobilize CD34+ progenitor cells from the bone marrow to the peripheral blood, then isolated using anti-CD34 magnetic beads) (#mPB016F, AllCells, Alameda,Full Paper
2020Yu, Z;Yin, S;Zhang, W;Jiang, X;Hu, J;Picosecond laser texturing on titanium alloy for biomedical implants in cell proliferation and vascularizationJournal of biomedical materials research. Part B, Applied biomaterials31692202Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCells Biological Technology Limited company and cultured at 1 × 105 on plates with Dulbecco's Modified Eagle's medium supplemented with 10% fetal bovine serum, 100 U/ml streptomycFull Paper
2020Bhargav, A;Min, KS;Wen Feng, L;Fuh, JYH;Rosa, V;Taguchi's methods to optimize the properties and bioactivity of 3D printed polycaprolactone/mineral trioxide aggregate scaffold: Theoretical predictions and experimental validationJournal of biomedical materials research. Part B, Applied biomaterials31112004The use of human dental pulp stem cells (DPSC) from single donor (DPF003, Allcells, Alameda) was approved by NUS Institutional Review Board (Approval Number: NUS 2094). The characterization and expressions of CD34, CD73, CD90, CD105 are available elsFull Paper
2020Al-Amoodi, AS;Sakashita, K;Ali, AJ;Zhou, R;Lee, JM;Tehseen, M;Li, M;Belmonte, JCI;Kusakabe, T;Merzaban, JS;Using Eukaryotic Expression Systems to Generate Human α1,3-Fucosyltransferases That Effectively Create Selectin-Binding Glycans on Stem CellsBiochemistry32901486leukemia cell line), THP1 (human acute monocytic leukemia), HL60 (human acute promyelocytic leukemia) (ATCC), and MSCs (primary mesenchymal stromal cells) (AllCells) were SaveFull Paper
2020Behbehani, GK;Finck, R;Samusik, N;Sridhar, K;Fantl, WJ;Greenberg, PL;Nolan, GP;Profiling myelodysplastic syndromes by mass cytometry demonstrates abnormal progenitor cell phenotype and differentiationCytometry. Part B, Clinical cytometry31917512in Table S2, with the risk‐based clinical status of MDS patients indicated by IPSS category and morphologic characteristics (Greenberg et al., 1997). Five healthy control samples were obtained from Allcells, Inc. using the same protocol. Normal boneFull Paper
2020Kim, DH;Kim, DH;Heck, BE;Shaffer, M;Hur, J;Yoo, KH;A natural supplement formula reduces anti-oxidative stress and enhances osteo-chondrogenic differentiation potential in mesenchymal stem cellsJournal of clinical biochemistry and nutrition32523247rozen mononuclear cells from human bone marrow were purchased (AllCells, Emeryville, CA) and used to isolate human bone marrow-mesenchymal stem cells (hBM-MSCs). Briefly, thawed mononuclear cells were cultured in Dulbecco’s modified Eagle medium (DMEFull Paper
2020Wang, P;Qin, W;Liu, T;Jiang, D;Cui, L;Liu, X;Fang, Y;Tang, X;Jin, H;Qian, Q;PiggyBac-engineered T cells expressing a glypican-3-specific chimeric antigen receptor show potent activities against hepatocellular carcinomaImmunobiology31522780Human peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from AllCells (AllCells, Silicon, USA). 5 × 106−1 × 107 PBMCs were electroporated with 6 μg of GPC3CAR plasmid or an equal quantity of MOCK plasmid in an electroporatFull Paper
2020Liu, H;Gemmell, L;Lin, R;Zuo, F;Balfour, HH;Woo, JC;Hayes, GM;Development of an Improved Epstein-Barr Virus (EBV) Neutralizing Antibody Assay to Facilitate Development of a Prophylactic gp350-Subunit EBV VaccineMediterranean journal of hematology and infectious diseases32180911Healthy human donor serum samples were purchased from AllCells, LLC and Bioreclamation, LLC (n=39). Samples were heated at 56 oC for 30 minutes to inactivate complement, and SaveFull Paper
2020Chien, KS;Class, CA;Montalban-Bravo, G;Wei, Y;Sasaki, K;Naqvi, K;Ganan-Gomez, I;Yang, H;Soltysiak, KA;Kanagal-Shamanna, R;Do, KA;Kantarjian, HM;Garcia-Manero, G;LILRB4 expression in chronic myelomonocytic leukemia and myelodysplastic syndrome based on response to hypomethylating agentsLeukemia & lymphoma32036728BM samples from healthy individuals were obtained from AllCells (Emeryville, CA, USA). CD34+ cells were isolated using the CD34 MicroBead Kit (Miltenyi Biotec, Bergisch Gladback, SaveFull Paper
2020Wu, T;The mean ± 1 SD was calculated for triplicate cultures. The laboratory (ReachBio LLC, Seattle, Washington) was blinded to the identify of the test compounds and docetaxel served as the positive control, with blank and vehicle as negative controls.ThesisThe surviving mice of the “young adult” group at day 35 post-TBI were sacrificed and 5×104 BM cells acquired from femurs were plated in ColonyGEL Mouse Complete Medium (Reach Bio, Seattle, WA) as previously described [131, 135]. After 7 days of incubFull Paper
2020White, CM;Haidekker, MA;Kisaalita, WS;Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D CulturesSLAS technology31997709Human bone marrow derived (Tulane Center for Gene Therapy) 3.2 ± 1.4 AFM indentation Spread morphology on glass coverslips; cytoplasm measured; spherical AFM tip 76 Human bone marrow derived (AllCells) 33 ± 7 AFM indentation Spread morphology on TherFull Paper
2020Chen, Y;NOVEL SIGNALS IN STRESS ERYTHROPOIESIS: COORDINATED CONTROL OF WNT, PGE2 AND PERK SIGNALING PATHWAYS REGULATES EXPANSION AND DIFFERENTIATION OF STRESS ERYTHROID PROGENITORSThesisHuman bone marrow mononuclear cells (MNCs) (ReachBio) were thawed at 37°C water according to the ReachBio instructions. MNCs were enumerated and cell concentrations were adjusted to 5  105 cells/mL. MNCs were cultured in Gibco RPMI-1640 (InvitrogenFull Paper
2020Wu, JL;McIntyre, PW;Hong, JM;Yassen, GH;Bruzzaniti, A;Effects of radiopaque double antibiotic pastes on the proliferation, alkaline phosphatase activity and mineral deposition of dental pulp stem cellsArchives of oral biology32485262Human DPSC were purchased from ALLCells (Alameda, CA, USA), passaged once and aliquots stored in liquid nitrogen. For each experiment, DPSC were thawed and seeded into 10-cm2 dishes and cultured in α-MEM (HyClone Laboratories Inc., South Logan, UT, UFull Paper
2020Madanagopal, TT;Franco-Obregón, A;Rosa, V;Comparative study of xeno-free induction protocols for neural differentiation of human dental pulp stem cells in vitroArchives of oral biology31600663The DPSC were isolated from the third molar from single donor (DPF003, AllCells, USA). Due to ethical requirements, the age, gender and ethnic group of the donor cannot be disclosed SaveFull Paper
2020Pieles, O;Reck, A;Morsczeck, C;High endogenous expression of parathyroid hormone-related protein (PTHrP) supports osteogenic differentiation in human dental follicle cellsHistochemistry and cell biology32710187Human dental follicle cells (DFCs), which were purchased from AllCells, were cultivated in DMEM (Dulbecco's modified Eagle medium) supplemented with 10% fetal bovine serum and SaveFull Paper
2020Fujishiro, A;Iwasa, M;Fujii, S;Maekawa, T;Andoh, A;Tohyama, K;Takaori-Kondo, A;Miura, Y;Menatetrenone facilitates hematopoietic cell generation in a manner that is dependent on human bone marrow mesenchymal stromal/stem cellsInternational journal of hematology32572826Normal human BM samples were purchased from AllCells (Alameda, CA). To confirm consistency among donors, multiple batches of BM-MSCs were isolated as described previously [ SaveFull Paper
2020Yu, CI;Marches, F;Wu, TC;Martinek, J;Palucka, K;Techniques for the generation of humanized mouse models for immuno-oncologyMethods in enzymology32178826Adult CD34 + HPCs from G-CSF mobilized peripheral blood or bone marrow may be obtained from Allcells (Alameda, CA, USA) or Lonza (Basel, Switzerland). Cord blood CD34 + SaveFull Paper
2020Enezei, H;Qabbani, A;Ahmad, A;Khamis, M;Hassani, A;Hamad, H;The Effect of Strontium on Osteoblastogenesis and Osteoclastogenesis in Dental Stem Cells-induced Epidermal Growth Factor at Molecular Level: In Vitro StudyJournal of Hard Tissue BiologyAllCells, LLC Company, Emeryville, CA, USA Dental Stem Cells (DSCs) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Dulbecco’s modified Eagles medium (DMEM) high glucose(4.5g/l) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Fetal bovine serFull Paper
2020Kuang, Z;Li, L;Zhang, P;Chen, B;Wu, M;Ni, H;Yi, S;Zou, J;Liu, J;A novel antibody targeting TIM-3 resulting in receptor internalization for cancer immunotherapyAntibody therapeutics33928230The antibody was purified in-house (Innovent Biologics Co., Ltd., Suzhou, China) from HEK293 cells with either transient or stable expression unless indicated otherwise. Cell culture Human PBMCs (AllCells, Alameda, CA, USA) were cultured in AIM V MFull Paper
2020Moussi, K;Abusamra, DB;Yassine, O;Merzaban, J;Kosel, J;Strain-induced Differentiation of Mesenchymal Stem CellsAnnual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference33018453Human bone marrow derived mesenchymal stem/stromal cells (hMSCs) were purchased from AllCells. All the experiments were performed using MSCs in their 3-5th passage. An SaveFull Paper
2020Yang, Z;Wang, T;Wu, D;Min, Z;Tan, J;Yu, B;RNA N6-methyladenosine reader IGF2BP3 regulates cell cycle and angiogenesis in colon cancerJournal of experimental & clinical cancer research : CR32993738lls were cultured in RPMI 1640 medium supplemented with 10% FBS (Invitrogen, Carlsbad, CA, USA) at 37 °C in a 5% CO2 atmosphere. Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from Allcells, Inc. (Alameda, CA, USA) and cultured in EndFull Paper
2020MacGlashan, D;Modulating the Human Basophil Phenotype During Its Development and Maturation: Basophils Derived from In Vitro Cultures of CD34+ Progenitor CellsMethods in molecular biology (Clifton, N.J.)32766967CD34+ progenitor stems (commercially prepared, multiple sources, e.g., AllCells (Quincy, MA, USA), or Fred Hutchinson Cancer Research Center (Seattle, WA, USA)). 12. FBS (fetal bovine serum) (multiple sources, heat-inactivated). 13. Penicillin/streFull Paper
2020Urkasemsin, G;Rungarunlert, S;Ferreira, JN;Bioprinting Strategies for Secretory Epithelial OrganoidsMethods in molecular biology (Clifton, N.J.)32207117hDPSC lines are commercially available through AllCells (Alameda, CA, USA) and should be used at lower passage to guarantee that their proliferation rate and phenotype are stable. 2. The same lot of FBS should be utilized during the all experiments.Full Paper
2020Han, SS;Kang, SW;Metabolic Labeling of Live Stem Cell for In Vitro Imaging and In Vivo TrackingMethods in molecular biology (Clifton, N.J.)30997638Stem cell therapy offers promising solutions to diseases and injuries that traditional medicines and therapies can't effectively cure. To get and explain their full therapeutic potentials, the SaveFull Paper
2020Al-Mohannadi, A;Deola, S;Malki, A;Visualization of Factor Viii with Flow-Cytometry as a tool for Novel Gene Therapy Approach in Hemophilia AUniversity of the Future: Re-Imagining Research and Higher Educationreceived as a kind gift from Antonia Follenzi, Health Sciences Department in Piemonte Orientale University in Novara-Italy. HELA cells (cervical cancer) were purchased from American Type Culture Collection (ATCC™), USA. PBMCs purchased by AllCells (CFull Paper
2020Romito, M;Juillerat, A;Kok, Y;HIldenbeutel, M;Rhiel, M;Andrieux, G;Geiger, J;Rudolph, C;Mussolino, C;Duclert, A;Metzner, K;Duchateau, P;Cathomen, T;Cornu, T;Clinically relevant CCR5 editing in primary CD4+ T cells with TALEN confers resistance to HIV-1 infectionAuthorea, Inc.For oligonucleotide capture assay (OCA), human PBMCs (ALLCELLS, USA) were plated at a density of 1x106 cells/ml in X-vivo-15 media (Lonza, Switzerland) supplemented with 5% SaveFull Paper
2020Turchiano, G;Andrieux, G;Blattner, G;Pennucci, V;Klermund, J;Monaco, G;Poddar, S;Mussolino, C;Cornu, T;Boerries, M;Cathomen, T;Quantitative Evaluation of Chromosomal Rearrangements in Primary Gene-Edited Human Stem Cells by Preclinical CAST-SeqSSRN Electronic JournalCell culture and transfection. Cryopreserved human CD34+ HSPCs derived from cord blood (AllCells, Cat.# CB008F; StemCell-Technologies, Cat.# 70008.3) were thawed and cultivated in a density range of 0.5-1 x 106 /ml at 37°C, 5% CO2 in GMP-grade CellGFull Paper
2020Lu, B;Yang, H;Liu, F;Sun, Y;He, H;Ding, X;Hu, S;xia, l;Escherichia coli Nissle 1917 secreting functional interleukin 2 targets tumours and enhances the immune response to suppress tumoursResearch SquareCT26 colon carcinoma lines were maintained by our laboratory, and peripheral blood mononuclear cells (PBMCs) were purchased from Allcells Biotechnology (Shanghai) Co., Ltd. SaveFull Paper
2020Zhang, Y;Wang, P;wang, t;Fang, Y;Ding, Y;Qian, Q;Chimeric Antigen Receptor T Cells Engineered to Secrete CD40 Agonist Antibodies Enhance Antitumor EfficacyResearch SquareHuman peripheral blood mononuclear cells (PBMC) from healthy donors were purchased from AllCells (Shanghai, China) and cryopreserved in our laboratory. SaveFull Paper
2020Mangiras, D;Mattheakis, P;Ribet, P;Dimitrakopoulos, G;Soft-Clustering Driven Flip-flop Placement Targeting Clock-induced OCVProceedings of the 2020 International Symposium on Physical DesignIn this way, the cluster initialization of clock net n is done on AllCells that includes both the cells connected to n and the pre-defined cluster centers of the hierarchically lower clock sub- SaveFull Paper
2020Li, W;Yang, S;Xu, P;Zhang, D;Tong, Y;Chen, L;Jia, B;Li, A;Ru, D;Zhang, B;Liu, M;Lian, C;Chen, C;Fu, W;Yuan, S;Ren, X;Liang, Y;Yang, Z;Li, W;Wang, S;Zhang, X;Lu, H;Xu, J;Wang, H;Yu, W;Human Identical Sequences of SARS-CoV-2 Promote Clinical Progression of COVID-19 by Upregulating Hyaluronan via NamiRNA-Enhancer NetworkbioRxivHUVEC (human umbilical vein endothelial cell, bought from AllCells) was cultured in the commercial culture medium (AllCells, H-004) according to the manufacturer's guidelines. SaveFull Paper
2020Fiskin, E;Lareau, C;Eraslan, G;Ludwig, L;Regev, A;Single-cell multimodal profiling of proteins and chromatin accessibility using PHAGE-ATACbioRxivFor PBMC and CD8 T cell experiments, cryopreserved PBMCs or CD8 T cells (AllCells) were thawed, washed in PBS and resuspended in cold Flow cytometry buffer (FC buffer; PBS containing 2% FBS). All centrifugation steps were carried out at 350g, 4min, 4Full Paper
2020Mimitou, E;Lareau, C;Chen, K;Zorzetto-Fernandes, A;Takeshima, Y;Luo, W;Huang, T;Yeung, B;Thakore, P;Wing, J;Nazor, K;Sakaguchi, S;Ludwig, L;Sankaran, V;Regev, A;Smibert, P;Scalable, multimodal profiling of chromatin accessibility and protein levels in single cellsbioRxivCryopreserved healthy donor peripheral blood mononuclear cells (PBMCs) and bone marrow cells (BM) were obtained from AllCells (USA) or Cellular Technology Limited (CTL) and processed immediately after thawing. NIH-3T3 and HEK293FT cells were maintainFull Paper
2020Steinbuck, M;Seenappa, L;Jakubowski, A;McNeil, L;Haqq, C;DeMuth, P;A Lymph Node Targeted Amphiphile Vaccine Induces Potent Cellular and Humoral Immunity to SARS-CoV-2bioRxivSARS-CoV-2 infection (COVID-19) were obtained from US Biolab (Rockville, MD) and ALLCELLS (Alameda, CA), respectively. All samples were received and stored frozen at -80°C until analysisFull Paper
2020Keating, S;Mizrahi, R;Adams, M;Asensio, M;Benzie, E;Carter, K;Chiang, Y;Edgar, R;Gautam, B;Gras, A;Leong, J;Leong, R;Lim, Y;Manickam, V;Medina-Cucurella, A;Niedecken, A;Saini, J;Simons, J;Spindler, M;Stadtmiller, K;Tinsley, B;Wagner, E;Wayham, N;Tracy, L;Lundberg, C;Büscher, D;Terencio, J;Roalfe, L;Pearce, E;Richardson, H;Goldblatt, D;Ramjag, A;Carrington, C;Simmons, G;Muench, M;Chamow, S;Monroe, B;Olson, C;Oguin, T;Lynch, H;Jeanfreau, R;Meyer, E;Adler, A;Johnson, D;Capturing and Recreating Diverse Antibody Repertoires as Multivalent Recombinant Polyclonal Antibody DrugsbioRxivPlasma-derived polyclonal antibodies are polyvalent drugs used for many important clinical indications that require modulation of multiple drug targets simultaneously, including SaveFull Paper
2020Sharma, R;Dever, D;Lee, C;Azizi, A;Pan, Y;Camarena, J;Köhnke, T;Bao, G;Porteus, M;Majeti, R;TRACE-Seq Reveals Clonal Reconstitution Dynamics of Gene Targeted Human Hematopoietic Stem CellsbioRxivFor in vivo studies presented, cord blood-derived CD34 + cells were purchased from AllCells or Stemcell Technologies and were thawed according to the manufacturer's SaveFull Paper
2020Miller, I;Sun, L;Harris, A;Gamboa, L;Zamat, A;Kwong, G;Remote control of CAR T cell therapies by thermal targetingbioRxivPrimary Human CD3+ cells were obtained from an anonymous donor blood after apheresis (AllCells) and were cryopreserved in 90% FBS and 10% DMSO until subsequent use. After SaveFull Paper
2020Mandal, S;Sunagawa, S;Prathipati, P;Belshan, M;Shibata, A;Destache, C;Targeted immuno-antiretroviral HIV therapeutic approach to provide dual protection and boosts cellular immunity: A proof-of-concept studybioRxivHuman immunodeficiency virus (HIV)-infected active and latent CCR5 expressing long-lived T-cells are the primary barrier to HIV/AIDS eradication. Broadly neutralizing antibodies and SaveFull Paper
2020Burley, D;Campagna, C;Harte, A;Kelly, S;Spiegelhoff, M;Effects of Erythrocyte Aggregation on Blood Rheology in Regard to Future Sepsis Diagnosis ApplicationThesisThe preparation of the blood dextran solutions for the micro-ESR, capillary fill, and vibration syllectometry were all done as one big batch. Blood was obtained from AllCells, LLC, a reputable blood and marrow cell supplier from Quincy, MassachusettsFull Paper
2020Fleischer, LC;Engineering Novel Chimeric Antigen Receptors (CARs) for T-cell Malignancies Using Innate Immune CellsThesisMethods and Materials γδ T-cell expansion. gd T cells were expanded from cryopreserved PBMCs purchased from AllCells (Alameda, California) or from fresh PBMCs isolated from 30-50 mL consented, healthy adult blood. PBMCs were isolated from blood usingFull Paper
2020O'Brien, G;Genetic and epigenetic consequences of radiation exposure in human and mouse leukaemogenesisThesisGreece with informed consent from each patient. Bone marrow aspirates were obtained from 5 normal donors (ALLCELLS, Alameda, CA) with informed consent and ethical approval from the Alpha Independent Review Board. Bone marrow aspirates from 7 AML patiFull Paper
2020Kimmey, SC;Mapping Single Cell Protein & Biosynthesis Dynamics Across Human Embryonic & Adult Stem Cell SpecificationThesisFresh bone marrow aspirates from two independent donors were obtained from AllCells (Alameda, CA) on the same day of collection, and immediately after receiving was placed in 37C 5% CO2 incubator for 30 minutes prior to SOM3B labeling. A mixture of aFull Paper
2020Davis-Marcisak, E;Fitzgerald, A;Kessler, M;Danilova, L;Jaffee, E;Zaidi, N;Weiner, L;Fertig, E;Transfer learning between preclinical models and human tumors identifies conserved NK cell activation signature in anti-CTLA-4 responsive tumorsbioRxivFresh healthy donor NK cells were purchased from AllCells (PB012-P). These NK cells were positively selected from donor peripheral blood using CD56 positivity. Donor NK cell purity SaveFull Paper
2020Burgenson, D;Development of a Cell-Free Protein Expression System Derived from Human Blood CellsThesisA comparison between methods used to produce leukapheresis products purchased from Allcells and the New York Blood Center. From the experiments performed, the Allcells SaveFull Paper
2020Yun, ZX;Identification of G9a as a Therapeutically Targetable Driver of Multiple Myeloma TumorigenesisThesisdissociate cells each passage. Cryopreserved Peripheral Blood Mononuclear Cells (PBMC, AllCells) were cultured in RPMI 1640 medium (BioWest, Kansas City, MO) with supplement of 10% FBS (HyClone) and 1% penicillin/streptomycin (Invitrogen) one day befFull Paper
2020Lindgren, A;Mohidin, D;Applying Digital Marketing Methods in the Healthcare Industry: A Case Study at ImmuneedThesisImmuneed Volume Immuneed Traffic Celentyx Volume Celentyx Traffic Reachbio Volume Reachbio Traffic Eurofins Volume Eurofins Traffic Immundnz Volume Immundnz Traffic Nexelis Volume Nexelis Traffic ImmuneCarta Volume ImmuneCarta TrafficFull Paper
2020Su, R;Dong, L;Li, Y;Gao, M;Han, L;Wunderlich, M;Deng, X;Li, H;Huang, Y;Gao, L;Li, C;Zhao, Z;Robinson, S;Tan, B;Qing, Y;Qin, X;Prince, E;Xie, J;Qin, H;Li, W;Shen, C;Sun, J;Kulkarni, P;Weng, H;Huang, H;Chen, Z;Zhang, B;Wu, X;Olsen, MJ;Müschen, M;Marcucci, G;Salgia, R;Li, L;Fathi, AT;Li, Z;Mulloy, JC;Wei, M;Horne, D;Chen, J;Targeting FTO Suppresses Cancer Stem Cell Maintenance and Immune EvasionCancer cell32531268After 7 days of selection with 0.5mg/ml G418 Sulfate (10131027, Thermo Fisher Scientific) in ColonyGEL (1201, ReachBio Research Lab), the cells were collected and injected into lethally irradiated (960 rads) 8- to 10-week-old B6.SJL (CD45.1, RRID: IMFull Paper
2020Young, TM;Reyes, C;Pasnikowski, E;Castanaro, C;Wong, C;Decker, CE;Chiu, J;Song, H;Wei, Y;Bai, Y;Zambrowicz, B;Thurston, G;Daly, C;Autophagy protects tumors from T cell-mediated cytotoxicity via inhibition of TNFα-induced apoptosisScience immunology33443027For human tumor cell killing experiments using CD3 bispecific antibody, human T cells were isolated from peripheral blood mononuclear cells (PBMCs) (ReachBio) using Dynabeads Untouched Human T Cells Kit (Thermo Fisher Scientific)Full Paper
2020Shen, C;Sheng, Y;Zhu, AC;Robinson, S;Jiang, X;Dong, L;Chen, H;Su, R;Yin, Z;Li, W;Deng, X;Chen, Y;Hu, YC;Weng, H;Huang, H;Prince, E;Cogle, CR;Sun, M;Zhang, B;Chen, CW;Marcucci, G;He, C;Qian, Z;Chen, J;RNA Demethylase ALKBH5 Selectively Promotes Tumorigenesis and Cancer Stem Cell Self-Renewal in Acute Myeloid LeukemiaCell stem cell32402250Thereafter, the transduced cells were then plated into ColonyGEL methylcellulose medium (ReachBio, Seattle, WA) supplied with 10 ng/ml of murine recombinant IL-3, IL-6, GM-CSF and 30 ng/ml of murine recombinant SCF, along with 1.0 mg/ml of G418 (GibcFull Paper
2020Chiu, D;Tavaré, R;Haber, L;Aina, OH;Vazzana, K;Ram, P;Danton, M;Finney, J;Jalal, S;Krueger, P;Giurleo, JT;Ma, D;Smith, E;Thurston, G;Kirshner, JR;Crawford, A;A PSMA-Targeting CD3 Bispecific Antibody Induces Antitumor Responses that Are Enhanced by 4-1BB CostimulationCancer immunology research32184296PBMCs were obtained from ReachBio (WA) for in vivo (ReachBio). C4-2 tumor cells were subcutaneously injected with 5 × 10 6 tumor cells and 1 × 10 6 human PBMCs (ReachBio). SaveFull Paper
2020Patterson, AM;Liu, L;Sampson, CH;Plett, PA;Li, H;Singh, P;Mohammad, KS;Hoggatt, J;Capitano, ML;Orschell, CM;Pelus, LM;A Single Radioprotective Dose of Prostaglandin E2 Blocks Irradiation-Induced Apoptotic Signaling and Early Cycling of Hematopoietic Stem CellsStem cell reports32735825CFCs were quantitated as described by Hoggatt et al. (2013a). TNCs from non-IR mice were plated at 2 × 104 per 35-mm dish in triplicate in ColonyGEL Mouse Complete Medium (Reach Bio, Seattle, WA). Cells from IR mice were plated at 0.5-1 × 106 per disFull Paper
2020Paulson, RF;Hariharan, S;Little, JA;Stress erythropoiesis: definitions and models for its studyExperimental hematology32750404Control PBMCs were purchased from ReachBio. PBMCs obtained were plated at 1 x 10 6 cells/ml stress erythropoiesis differentiation media containing human growth factors. The cells SaveFull Paper
2020Patterson, AM;Plett, PA;Chua, HL;Sampson, CH;Fisher, A;Feng, H;Unthank, JL;Miller, SJ;Katz, BP;MacVittie, TJ;Orschell, CM;Development of a Model of the Acute and Delayed Effects of High Dose Radiation Exposure in Jackson Diversity Outbred Mice; Comparison to Inbred C57BL/6 MiceHealth physics32932286Colony-forming unit cell (CFU-C) analyses Functional BM progenitor number was analyzed utilizing methylcellulose colony assays as per manufacturer instructions (Colony Gel 1202, ReachBio, Seattle, WA)Full Paper
2020Radtke, S;Colonna, L;Perez, AM;Hoffman, M;Kean, LS;Kiem, HP;Isolation of a Highly Purified HSC-enriched CD34+CD90+CD45RA- Cell Subset for Allogeneic Transplantation in the Nonhuman Primate Large-animal ModelTransplantation direct33134503CFC) assays were sorted in purity mode to prevent crosscontamination by different subsets. Colony-forming Cell Assay For CFC assays, 1000 to 1200 sorted cells were seeded into 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA). Hematopoietic colonies werFull Paper
2019Bonner, M;Kanter, J;Macari, E;Lane, R;Lewis, G;Coles, P;Kassenaar, S;Mynampati, S;Schulze, R;Hebert, M;Walters, M;Thompson, A;Asmal, M;Tisdale, J;Pierciey, F;The Relationships between Target Gene Transduction, Engraftment of HSCs and RBC Physiology in Sickle Cell Disease Gene TherapyBloodBackground LentiGlobin for Sickle Cell Disease (SCD) gene therapy contains ex-vivo lentiviral vector (LVV)-mediated addition of a modified β-globin gene (βA-T87Q) into autologous CD34+ hematopoietic stem cells (HSCs). The safety and efFull Paper
2019Kanter, J;Tisdale, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Schmidt, M;Miller, A;Pierciey, F;Huang, W;Ribeil, J;Thompson, A;Walters, M;Resolution of Sickle Cell Disease Manifestations in Patients Treated with Lentiglobin Gene Therapy: Updated Results from the Phase 1/2 Hgb-206 Group C StudyBloodBackground β-globin gene transfer into hematopoietic stem cells (HSCs) could reduce or eliminate sickle cell disease (SCD)-related manifestations. LentiGlobin for SCD gene therapy contains autologous CD34+ cells transduced with the BB3Full Paper
2019Walters, M;Tisdale, J;Kwiatkowski, J;Krishnamurti, L;Mapara, M;Schmidt, M;Miller, A;Pierciey, F;Huang, W;Ribeil, J;Kanter, J;Thompson, A;Exploring the Drivers of Potential Clinical Benefit in Initial Patients Treated in the Hgb-206 Study of Lentiglobin for Sickle Cell Disease (SCD) Gene TherapyBloodBackground LentiGlobin for SCD gene therapy contains autologous CD34+ hematopoietic stem cells (HSCs) transduced with the BB305 lentiviral vector (LVV) encoding β-globin with an anti-sickling substitution (T87Q). Its safety and efficacFull Paper
2019Eapen, M;Neuberg, D;Mendizabal, A;Stevenson, K;Antin, J;DiFronzo, N;El Rassi, F;Lulla, P;Waller, E;Garrison, J;Smith, S;Sullivan, K;Walters, M;Krishnamurti, L;A Phase II Trial to Compare Allogeneic Transplant Vs. Standard of Care for Severe Sickle Cell Disease: Blood and Marrow Transplant Clinical Trials Network (BMT CTN) Protocol 1503BloodBackground: Allogeneic hematopoietic cell transplantation (HCT) is a curative treatment for sickle cell disease (SCD). A pilot trial confirmed the suitability of a myeloablative conditioning regimen (busulfan/fludarabine/r-ATG) for HLA-matched siblinFull Paper
2019Loi, S;Giobbie-Hurder, A;Gombos, A;Bachelot, T;Hui, R;Curigliano, G;Campone, M;Biganzoli, L;Bonnefoi, H;Jerusalem, G;Bartsch, R;Rabaglio-Poretti, M;Kammler, R;Maibach, R;Smyth, M;Di Leo, A;Colleoni, M;Viale, G;Regan, M;André, F;Fumagalli, D;Gelber, R;Goulioti, T;Hiltbrunner, A;Hui, R;Roschitzki, H;Ruepp, B;Boyle, F;Stahel, R;Aebi, S;Coates, A;Goldhirsch, A;Karlsson, P;Kössler, I;Fournarakou, S;Gasca, A;Pfister, R;Ribeli-Hofmann, S;Weber, M;Celotto, D;Comune, C;Frapolli, M;Sánchez-Hohl, M;Huang, H;Mahoney, C;Price, K;Scott, K;Shaw, H;Fischer, S;Greco, M;King, C;Andrighetto, S;Piccart-Gebhart, M;Findlay, H;Jenkins, M;Karantza, V;Mejia, J;Schneier, P;Pembrolizumab plus trastuzumab in trastuzumab-resistant, advanced, HER2-positive breast cancer (PANACEA): a single-arm, multicentre, phase 1b–2 trialThe Lancet OncologyPD-L1 status for the first 79 patients was assessed with the QualTek immunohistochemistry assay (QualtTek Molecular Laboratories, Santa Barbara, CA, USA) in which PD-L1 staining in at least 1% of tumour cells or any staining in stroma were defined asFull Paper
2019Kassim, A;Walters, M;Eapen, M;Allison, B;Mendizabal, A;Brodsky, R;DeBaun, M;Reduced Intensity Conditioning for Haploidentical Bone Marrow Transplantation in Patients with Symptomatic Sickle Cell Disease: BMT CTN Protocol 1507BloodBackground: Hematopoietic cell transplantation (HCT) has curative potential in sickle cell disease (SCD). A reduced intensity haploidentical bone marrow transplant (haplo-BMT) platform piloted by investigators at Johns Hopkins, addressed key barriersFull Paper
2019Nghiem, P;Bhatia, S;Lipson, EJ;Sharfman, WH;Kudchadkar, RR;Brohl, AS;Friedlander, PA;Daud, A;Kluger, HM;Reddy, SA;Boulmay, BC;Riker, AI;Burgess, MA;Hanks, BA;Olencki, T;Margolin, K;Lundgren, LM;Soni, A;Ramchurren, N;Church, C;Park, SY;Shinohara, MM;Salim, B;Taube, JM;Bird, SR;Ibrahim, N;Fling, SP;Homet Moreno, B;Sharon, E;Cheever, MA;Topalian, SL;Durable Tumor Regression and Overall Survival in Patients With Advanced Merkel Cell Carcinoma Receiving Pembrolizumab as First-Line TherapyJ. Clin. Oncol.3072617521,22. PD-L1 IHC. PD-L1 staining (anti-PD-L1 clone 22C3; Merck Research Laboratories, Kenilworth, NJ) was performed at QualTek Molecular Laboratories (Newtown, PA) on formalin-fixed paraffin-embedded sections from pretreatment MCC biopsies, as previoFull Paper
2019Fedoriw, A;Rajapurkar, SR;O'Brien, S;Gerhart, SV;Mitchell, LH;Adams, ND;Rioux, N;Lingaraj, T;Ribich, SA;Pappalardi, MB;Shah, N;Laraio, J;Liu, Y;Butticello, M;Carpenter, CL;Creasy, C;Korenchuk, S;McCabe, MT;McHugh, CF;Nagarajan, R;Wagner, C;Zappacosta, F;Annan, R;Concha, NO;Thomas, RA;Hart, TK;Smith, JJ;Copeland, RA;Moyer, MP;Campbell, J;Stickland, K;Mills, J;Jacques-O'Hagan, S;Allain, C;Johnston, D;Raimondi, A;Porter Scott, M;Waters, N;Swinger, K;Boriack-Sjodin, A;Riera, T;Shapiro, G;Chesworth, R;Prinjha, RK;Kruger, RG;Barbash, O;Mohammad, HP;Anti-tumor Activity of the Type I PRMT Inhibitor, GSK3368715, Synergizes with PRMT5 Inhibition through MTAP LossCancer Cell31257072GSK3368715 was evaluated at 20, 5, 1.25, 0.3125 and 0.078 mM in a total of 10 patient samples. DLBCL patient cells from 10 unique donors were received as frozen samples from Conversant Bio. The human biological samples were sourced ethically and theiFull Paper
2019Leighl, NB;Hellmann, MD;Hui, R;Carcereny, E;Felip, E;Ahn, MJ;Eder, JP;Balmanoukian, AS;Aggarwal, C;Horn, L;Patnaik, A;Gubens, M;Ramalingam, SS;Lubiniecki, GM;Zhang, J;Piperdi, B;Garon, EB;Pembrolizumab in patients with advanced non-small-cell lung cancer (KEYNOTE-001): 3-year results from an open-label, phase 1 studyLancet Respir Med30876831NR=not reached. PD-L1=programmed death ligand 1. TPS=tumour proportion score. *PD-L1 TPS 1% on the basis of the QualTek assay (Molecular Laboratories, GoletaFull Paper
2019Melamed, Z;López-Erauskin, J;Baughn, MW;Zhang, O;Drenner, K;Sun, Y;Freyermuth, F;McMahon, MA;Beccari, MS;Artates, JW;Ohkubo, T;Rodriguez, M;Lin, N;Wu, D;Bennett, CF;Rigo, F;Da Cruz, S;Ravits, J;Lagier-Tourenne, C;Cleveland, DW;Premature polyadenylation-mediated loss of stathmin-2 is a hallmark of TDP-43-dependent neurodegenerationNat. Neurosci.30643298... titute for the kind contribution of the SIN18 vector. We thank B. Ren for providing the Illumina sequencing platform. We thank A. Goginashvili for valued input on the manuscript. We thank J. Nuovo at Phylogeny Inc. (Columbus, OH) for performing tFull Paper
2019Thompson, A;Walters, M;Kwiatkowski, J;Hongeng, S;Porter, J;Sauer, M;Thrasher, A;Thuret, I;Elliot, H;Tao, G;Colvin, R;Locatelli, F;Northstar-2: Updated Safety and Efficacy Analysis of Lentiglobin Gene Therapy in Patients with Transfusion-Dependent β-Thalassemia and Non-β0/β0 GenotypesBloodBackground Transfusion-dependent β-thalassemia (TDT) is treated with regular, lifelong red blood cell (RBC) transfusions and despite iron-chelating therapy, carries a risk of serious organ damage from iron overload and other complicatiFull Paper
2019Petermann, F;Pękowska, A;Johnson, CA;Jankovic, D;Shih, HY;Jiang, K;Hudson, WH;Brooks, SR;Sun, HW;Villarino, AV;Yao, C;Singleton, K;Akondy, RS;Kanno, Y;Sher, A;Casellas, R;Ahmed, R;O'Shea, JJ;The Magnitude of IFN-γ Responses Is Fine-Tuned by DNA Architecture and the Non-coding Transcript of Ifng-as1Mol. CellFor LCMV experiments RNA was isolated from sorted cells of LCMV-infected mice with the QIAGEN AllPrep Micro Kit. Libraries were prepared and sequenced by the HudsonAlpha Genomic Services Lab (paired-end, 150 bp reads). For all other experiments totalFull Paper
2019Kwiatkowski, J;Thompson, A;Rasko, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;Schmidt, M;Kletzel, M;Vichinsky, E;Deary, B;Chen, Y;Petrusich, A;Walters, M;Long-Term Clinical Outcomes of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia in the Northstar (HGB-204) StudyBloodBackground Patients with transfusion-dependent β-thalassemia (TDT) may experience transfusional iron overload and end-organ damage. While potentially curative, allogeneic hematopoietic stem cell (HSC) transplantation is limited by tranFull Paper
2019Furie, R;Werth, VP;Merola, JF;Stevenson, L;Reynolds, TL;Naik, H;Wang, W;Christmann, R;Gardet, A;Pellerin, A;Hamann, S;Auluck, P;Barbey, C;Gulati, P;Rabah, D;Franchimont, N;Monoclonal antibody targeting BDCA2 ameliorates skin lesions in systemic lupus erythematosusJ. Clin. Invest.30645203... un-exposed skin of HVs matched approximately by age and ethnicity to the BIIB059-treated cohort (mean age = 34; range, 28–37; White, 6/8; Asian, 1/8; Black or African American, 1/8; female, 8/8) by Folio Biosciences, then processed into paraffinFull Paper
2019Hudson, WH;Prokhnevska, N;Gensheimer, J;Akondy, R;McGuire, DJ;Ahmed, R;Kissick, HT;Expression of novel long noncoding RNAs defines virus-specific effector and memory CD8+ T cellsNat Commun30643116... of Emory University. RNA Isolation and sequencing RNA was isolated from sorted cells of LCMV-infected mice with the Qiagen AllPrep Micro Kit. Library preparation and sequencing were performed by the HudsonAlpha Genomic Services Laboratory via thFull Paper
2019Ma, X;Shao, Y;Tian, L;Flasch, DA;Mulder, HL;Edmonson, MN;Liu, Y;Chen, X;Newman, S;Nakitandwe, J;Li, Y;Li, B;Shen, S;Wang, Z;Shurtleff, S;Robison, LL;Levy, S;Easton, J;Zhang, J;Analysis of error profiles in deep next-generation sequencing dataGenome Biol.30867008Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA. Xiaotu.Ma@stjude.org.++Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.++Department of ComputatioFull Paper
2019Zeng, Q;Liao, C;Terhune, J;Wang, L;Impacts of florfenicol on the microbiota landscape and resistome as revealed by metagenomic analysisMicrobiome31818316Carlsbad, CA) according to the manufacturer's instructions. The library construction and sequencing were conducted at the HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). Genomic libraries were prepared with theFull Paper
2019Lee, CR;Yonk, AJ;Wiskerke, J;Paradiso, KG;Tepper, JM;Margolis, DJ;Opposing Influence of Sensory and Motor Cortical Input on Striatal Circuitry and Choice BehaviorCurr. Biol.30982651Light was delivered to the cortical afferents and PV interneurons in the striatum through an optical fiber patchcord (Thorlabs; length 1 m; 0.50 nA; 200 mm diameter) connected to an optical cannula (described above) via a mating sleeve (Thorlabs). AFull Paper
2019Ramachandran, I;Lowther, DE;Dryer-Minnerly, R;Wang, R;Fayngerts, S;Nunez, D;Betts, G;Bath, N;Tipping, AJ;Melchiori, L;Navenot, JM;Glod, J;Mackall, CL;D'Angelo, SP;Araujo, DM;Chow, WA;Demetri, GD;Druta, M;Van Tine, BA;Grupp, SA;Abdul Razak, AR;Wilky, B;Iyengar, M;Trivedi, T;Winkle, EV;Chagin, K;Amado, R;Binder, GK;Basu, S;Systemic and local immunity following adoptive transfer of NY-ESO-1 SPEAR T cells in synovial sarcomaJ Immunother Cancer31651363d Cross (Philadelphia, PA). NY-ESO-1 testing was performed via IHC at a Clinical Laboratory Improvement Amendments certified pathology laboratory at the National Cancer Institute (Bethesda, MD) or at QualTek Labs (Goleta, CA). Disease response was clFull Paper
2019Spino, M;Kurz, SC;Chiriboga, L;Serrano, J;Zeck, B;Sen, N;Patel, S;Shen, G;Vasudevaraja, V;Tsirigos, A;Suryadevara, CM;Frenster, JD;Tateishi, K;Wakimoto, H;Jain, R;Riina, HA;Nicolaides, TP;Sulman, EP;Cahill, DP;Golfinos, JG;Isse, K;Saunders, LR;Zagzag, D;Placantonakis, DG;Snuderl, M;Chi, AS;Cell Surface Notch Ligand DLL3 is a Therapeutic Target in Isocitrate Dehydrogenase-mutant GliomaClin. Cancer Res.30397180For the discovery set, 20 nontumor brain tissue samples and 63 glioma tumor samples were obtained from Cooperative Human Tumor Network (CHTN), Conversant Bio, and RS Diagnostics. This set of tumors included the following diagnoses: glioblastoma, WHOFull Paper
2019Habra, MA;Stephen, B;Campbell, M;Hess, K;Tapia, C;Xu, M;Rodon Ahnert, J;Jimenez, C;Lee, JE;Perrier, ND;Boraddus, RR;Pant, S;Subbiah, V;Hong, DS;Zarifa, A;Fu, S;Karp, DD;Meric-Bernstam, F;Naing, A;Phase II clinical trial of pembrolizumab efficacy and safety in advanced adrenocortical carcinomaJ Immunother Cancer31533818PD-L1 staining was performed by Qualtek using Merck 22C3 antibody for PD-L1 and scored by a board-certified pathologist. Based on the percentage and intensity of membrane staining, H-score, ranging from 0 to 300, was assigned to tumor samples ;iFull Paper
2019Lv, JW;Li, JY;Luo, LN;Wang, ZX;Chen, YP;Comparative safety and efficacy of anti-PD-1 monotherapy, chemotherapy alone, and their combination therapy in advanced nasopharyngeal carcinoma: findings from recent advances in landmark trialsJ Immunother Cancer31238988D-L1 expression was assessed baseline on an archived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy sample using a laboratory-developed prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA). PD-L1Full Paper
2019Clouthier, DL;Lien, SC;Yang, SYC;Nguyen, LT;Manem, VSK;Gray, D;Ryczko, M;Razak, ARA;Lewin, J;Lheureux, S;Colombo, I;Bedard, PL;Cescon, D;Spreafico, A;Butler, MO;Hansen, AR;Jang, RW;Ghai, S;Weinreb, I;Sotov, V;Gadalla, R;Noamani, B;Guo, M;Elston, S;Giesler, A;Hakgor, S;Jiang, H;McGaha, T;Brooks, DG;Haibe-Kains, B;Pugh, TJ;Ohashi, PS;Siu, LL;An interim report on the investigator-initiated phase 2 study of pembrolizumab immunological response evaluation (INSPIRE)J Immunother Cancer30867072IHC. For screening biopsies only, the FFPE blocks were used for PD-L1 IHC (clone 22C3) on 4-5 μm sections mounted on positively charged ProbeOn slides (QualTek, Goleta, CA). QualTek provided a modified proportion score ;from the same site as theFull Paper
2019Rowley, MJ;Lyu, X;Rana, V;Ando-Kuri, M;Karns, R;Bosco, G;Corces, VG;Condensin II Counteracts Cohesin and RNA Polymerase II in the Establishment of 3D Chromatin OrganizationCell Rep30865881We would like to thank the HudsonAlpha Institute for Biotechnology Genomic Services Lab for their help with Illumina sequencing. This work was supported by NIH Pathway to Independence Award K99/R00 GM127671 (M.J.R.) and U.S. Public Health Service AwaFull Paper
2019Wu, T;Dejanovic, B;Gandham, VD;Gogineni, A;Edmonds, R;Schauer, S;Srinivasan, K;Huntley, MA;Wang, Y;Wang, TM;Hedehus, M;Barck, KH;Stark, M;Ngu, H;Foreman, O;Meilandt, WJ;Elstrott, J;Chang, MC;Hansen, DV;Carano, RAD;Sheng, M;Hanson, JE;Complement C3 Is Activated in Human AD Brain and Is Required for Neurodegeneration in Mouse Models of Amyloidosis and TauopathyCell Rep31433986Human CSF cohort 1 Folio Biosciences n/a Human CSF cohort 2 PrecisionMed n/a Frozen human brain tissue Folio Biosciences n/Full Paper
2019Angelova, E;Audette, C;Kovtun, Y;Daver, N;Wang, SA;Pierce, S;Konoplev, SN;Khogeer, H;Jorgensen, JL;Konopleva, M;Zweidler-McKay, PA;Medeiros, LJ;Kantarjian, HM;Jabbour, EJ;Khoury, JD;CD123 expression patterns and selective targeting with a CD123-targeted antibody-drug conjugate (IMGN632) in acute lymphoblastic leukemiaHaematologica30361418... In vitro evaluation of primary B-cell acute lymphoblastic leukemia samples Bone marrow mononuclear cells from 11 newly diagnosed and 10 relapsed/refractory B-ALL patients were obtained from MDACC or ConversantBio. The number of CD123 antibody-biFull Paper
2019Bao, L;Tian, C;Liu, S;Zhang, Y;Elaswad, A;Yuan, Z;Khalil, K;Sun, F;Yang, Y;Zhou, T;Li, N;Tan, S;Zeng, Q;Liu, Y;Li, Y;Li, Y;Gao, D;Dunham, R;Davis, K;Waldbieser, G;Liu, Z;The Y chromosome sequence of the channel catfish suggests novel sex determination mechanisms in teleost fishBMC Biol.30683095;... Bioanalyzer. Equal amounts of RNA from each 5-day period were pooled from 10 to 14 dpf and 15 to 19 dpf for male and female, respectively. These four pooled samples were outsourced for RNA-Seq at the HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2019Rieker, C;Migliavacca, E;Vaucher, A;Mayer, FC;Baud, G;Marquis, J;Charpagne, A;Hegde, N;Guignard, L;McLachlan, M;Pooler, AM;Apolipoprotein E4 Expression Causes Gain of Toxic Function in Isogenic Human Induced Pluripotent Stem Cell-Derived Endothelial CellsArterioscler. Thromb. Vasc. Biol.31315437Immunohistochemistry Analysis of Postmortem Human Brain Tissue Human hippocampal brain tissue from AD (Braak stage V-VI; both male and female) individuals was purchased from Folio Biosciences, genotyped for ApoE, and processed for cryo- sectioningFull Paper
2019Smith, A;Schiller, G;Vercellotti, G;Kwiatkowski, J;Krishnamurti, L;Esrick, E;Williams, D;Miller, W;Woolfson, A;Walters, M;Preliminary Results of a Phase 1/2 Clinical Study of Zinc Finger Nuclease-Mediated Editing of BCL11A in Autologous Hematopoietic Stem Cells for Transfusion-Dependent Beta ThalassemiaBloodIntroduction: Persistently high fetal hemoglobin (HbF) expression can ameliorate severe transfusion-dependent beta thalassemia (TDT). BCL11A, a master regulator of the fetal-to-adult hemoglobin switch, is a rational gene-editing target in beta globinFull Paper
2019Kang, J;Yeom, G;Jang, H;Oh, J;Park, CJ;Kim, MG;Development of Replication Protein A-Conjugated Gold Nanoparticles for Highly Sensitive Detection of Disease BiomarkersAnal. Chem.31269392Patient samples for NP and cTnI were obtained from Discovery Life Sciences, Inc. The study protocol was thoroughly explained to the patients, and signed written informed consent forms were obtained in accordance with the approved guidelines and relevFull Paper
2019Planes-Laine, G;Rochigneux, P;Bertucci, F;Chrétien, AS;Viens, P;Sabatier, R;Gonçalves, A;PD-1/PD-L1 Targeting in Breast Cancer: The First Clinical Evidences Are Emerging. A Literature ReviewCancers (Basel)31336685ts included locally advanced or metastatic disease, after a failure of or inability to receive standard therapy, ECOG PS 0 or 1, ≥ 1 measurable lesion and PD-L1 positivity, using a prototype assay (QualTek Molecular Laboratories, Goleta, CA, USA) andFull Paper
2019Bernard-Gauthier, V;Mossine, AV;Knight, A;Patnaik, D;Zhao, WN;Cheng, C;Krishnan, HS;Xuan, LL;Chindavong, PS;Reis, SA;Chen, JM;Shao, X;Stauff, J;Arteaga, J;Sherman, P;Salem, N;Bonsall, D;Amaral, B;Varlow, C;Wells, L;Martarello, L;Patel, S;Liang, SH;Kurumbail, RG;Haggarty, SJ;Scott, PJH;Vasdev, N;Structural Basis for Achieving GSK-3β Inhibition with High Potency, Selectivity, and Brain Exposure for Positron Emission Tomography Imaging and Drug DiscoveryJ. Med. Chem.31535859For autoradiography experiments, fresh-frozen postmortem human brain tissue blocks were acquired from Folio-Conversant and the University of California at San Francisco NDBB. In vitro autoradiography studies were performed as described in the SupportFull Paper
2019McCoy, CR;Glover, ME;Flynn, LT;Simmons, RK;Cohen, JL;Ptacek, T;Lefkowitz, EJ;Jackson, NL;Akil, H;Wu, X;Clinton, SM;Altered DNA Methylation in the Developing Brains of Rats Genetically Prone to High versus Low AnxietyJ. Neurosci.30683683Starting material for the enrichment protocol was 1g of sonicated DNA, and captured fragments were eluted using 2500 mM NaCl per the manufacturer’s instructions and as described previously (Brinkman et al., 2010). Captured material was purified usingFull Paper
2019Chiu, H;Trisal, P;Bjorklund, C;Carrancio, S;Toraño, EG;Guarinos, C;Papazoglou, D;Hagner, PR;Beldi-Ferchiou, A;Tarte, K;Delfau-Larue, MH;Morschhauser, F;Ramsay, AG;Gandhi, AK;Combination lenalidomide-rituximab immunotherapy activates anti-tumour immunity and induces tumour cell death by complementary mechanisms of action in follicular lymphomaBr. J. Haematol.30767211Viably‐frozen PBMC from naive and relapsed/refractory FL and MZL patients were purchased from ConversantBio (Huntsville, AL, USA), ProteoGenex, (Inglewood, CA, USA) and BioreclammationIVT (Hicksville, NY, USA). Cell lines and cell cultureFull Paper
2019Liang, X;Zhuang, J;Löffler, FE;Zhang, Y;DeBruyn, JM;Wilhelm, SW;Schaeffer, SM;Radosevich, M;Viral and bacterial community responses to stimulated Fe(III)-bioreduction during simulated subsurface bioremediationEnviron. Microbiol.30773777Whole soil DNA was obtained by extracting both soil aggregates and associated pore water (Cápiro et al., 2014) using PowerLyser PowerSoil DNA isolation kit (MoBio Laboratories Inv. Carlsbad, CA) following the manufacturer’s instructions. The extracteFull Paper
2019Nowinski, B;Motard-Côté, J;Landa, M;Preston, CM;Scholin, CA;Birch, JM;Kiene, RP;Moran, MA;Microdiversity and temporal dynamics of marine bacterial dimethylsulfoniopropionate genesEnviron. Microbiol.30761723This article is protected by copyright. All rights reserved. Page 23. HiSeq Rapid Run at HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA). Metagenomic data are deposited in the NCBI SRA under project number PRJNA505827. Single AmplifiedFull Paper
2019Xu, H;Muise, ES;Javaid, S;Chen, L;Cristescu, R;Mansueto, MS;Follmer, N;Cho, J;Kerr, K;Altura, R;Machacek, M;Nicholson, B;Addona, G;Kariv, I;Chen, H;Identification of predictive genetic signatures of Cytarabine responsiveness using a 3D acute myeloid leukaemia modelJ. Cell. Mol. Med.314493472 METHODS. 2.1 Cells and reagents. Normal and AML patient BMMCs were purchased from Folio Conversant and ProteoGenex. Recombinant human fibronectin was purchased from Millipore. Rat tail collagen type 1 and matrigel were purchased from Corning IncFull Paper
2019Kim, YJ;Keam, B;Ock, CY;Song, S;Kim, M;Kim, SH;Kim, KH;Kim, JS;Kim, TM;Kim, DW;Lee, JS;Heo, DS;A phase II study of pembrolizumab and paclitaxel in patients with relapsed or refractory small-cell lung cancerLung Cancer31494530Informed consent for specimens be obtained during screening for protocol enrollment from all subjects or legally acceptable representative, at a trial visit by the investigator or designate. Pretreatment blood sampling and tumor biopsies will be doneFull Paper
2019Yang, X;Saito, Y;Rao, A;Kim, HJ;Singh, P;Scott, E;Larson, M;Pan, W;Desai, M;Hubbell, E;Alignment-free filtering for cfNA fusion fragmentsBioinformatics31510681We have obtained 40 ml of whole blood collected in Streck tubes, separated into plasma and extracted cfRNA samples from two Stage IV Prostate cancer patients and three healthy controls from Conversant Bio and generated cfRNA sequencing data with UMIsFull Paper
2019Qi, Z;Wang, L;Desai, K;Cogswell, J;Stern, M;Lawson, B;Kerkar, SP;Vitazka, P;Reliable Gene Expression Profiling from Small and Hematoxylin and Eosin-Stained Clinical Formalin-Fixed, Paraffin-Embedded Specimens Using the HTG EdgeSeq PlatformJ Mol Diagn31255795Rockville, MD). Small-cell lung cancer (SCLC) samples (n = 4) were purchased from Conversant Biologics, Inc. (Huntsville, AL) or Proteogenex (Inglewood, CA). FFPE muscle tissue was obtained from Proteogenex. RNA samplesFull Paper
2019Dietz, L;Dömel, JS;Leese, F;Mahon, AR;Mayer, C;Phylogenomics of the longitarsal Colossendeidae: The evolutionary history of an Antarctic sea spider radiationMol. Phylogenet. Evol.31002869Sea spiders (Pycnogonida) constitute a group of marine benthic arthropods that has a particularly high species diversity in the Southern Ocean. The "longitarsal" group of the sea spider family Colossendeidae is especially abundant in this region. HowFull Paper
2019Varga, A;Piha-Paul, S;Ott, PA;Mehnert, JM;Berton-Rigaud, D;Morosky, A;Yang, P;Ruman, J;Matei, D;Pembrolizumab in patients with programmed death ligand 1-positive advanced ovarian cancer: Analysis of KEYNOTE-028Gynecol. Oncol.30522700disease based on Response Evaluation Criteria in Solid Tumors version 1.1 (RECIST v1.1), PD-L1 positivity, determined immunohistochemically and defined as membranous staining on ≥1% modified proportion score or interface pattern (QualTek Molecular LaFull Paper
2019Lee, MJ;Miller, Z;Park, JE;Bhattarai, D;Lee, W;Kim, KB;H727 cells are inherently resistant to the proteasome inhibitor carfilzomib, yet require proteasome activity for cell survival and growthSci Rep30858500... on-linear regression based on n = 3 replicates per compound per concentration. (d) Heat map showing proteasome catalytic subunit activity profiles of 6 PI-naïve patient MM samples purchased from Conversant Biologics and AllCells (left). The numbeFull Paper
2019Liang, X;Radosevich, M;Löffler, F;Schaeffer, SM;Zhuang, J;Impact of microbial iron oxide reduction on the transport of diffusible tracers and non-diffusible nanoparticles in soilsChemosphere30597359Inc. Carlsbad, California, USA). The DNA samples were quantified using PicoGreen Assay Kit (Carlsbad, California, USA) and sent out for sequencing at HudsonAlpha Genomic Services Lab (Huntsville, Alabama, USA). TheFull Paper
2019Nicolini, F;Bocchini, M;Bronte, G;Delmonte, A;Guidoboni, M;Crinò, L;Mazza, M;Malignant Pleural Mesothelioma: State-of-the-Art on Current Therapies and Promises for the FutureFront Oncol32039010With a centrally-assessed 22C3 antibody-based immunohistochemistry assay (Qualtek laboratory), PDL1 expression was available in 62 patients: 45% were found negative (Full Paper
2019Gani, AW;Wei, W;Shi, RZ;Ng, E;Nguyen, M;Chua, MS;So, S;Wang, SX;An Automated, Quantitative, and Multiplexed Assay Suitable for Point-of-Care Hepatitis B Virus DiagnosticsSci Rep31666635HBV DNA levels). As controls, we recruited 5 healthy volunteers who had no prior history of HBV and who were vaccinated against HBV. Serum samples from healthy Asian donors were also purchased from Conversant Biologics (Huntsville, AL). SupplementaFull Paper
2019Li, Y;Kocot, KM;Tassia, MG;Cannon, JT;Bernt, M;Halanych, KM;Mitogenomics Reveals a Novel Genetic Code in HemichordataGenome Biol Evol30476024Variant codes in mitochondrial genomes within deuterostomes and photos from representatives of hemichordates. (A) Phylogenetic relationships according to the current understanding of deuterostome phylogeny based on multiple studies, red bars show codFull Paper
2019Juttukonda, LJ;Green, ER;Lonergan, ZR;Heffern, MC;Chang, CJ;Skaar, EP;Acinetobacter baumannii OxyR Regulates the Transcriptional Response to Hydrogen PeroxideInfect. Immun.30297527RNA-seq library construction and sequencing were performed by HudsonAlpha (Huntsville, AL). The concentration and integrity of the total RNA were estimated with a Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA) and an Agilent 2100 bioanalyzer (AppliFull Paper
2019Patel, S;Knight, A;Krause, S;Teceno, T;Tresse, C;Li, S;Cai, Z;Gouasmat, A;Carroll, VM;Barret, O;Gottmukkala, V;Zhang, W;Xiang, X;Morley, T;Huang, Y;Passchier, J;Preclinical In Vitro and In Vivo Characterization of Synaptic Vesicle 2A-Targeting Compounds Amenable to F-18 Labeling as Potential PET Radioligands for Imaging of Synapse IntegrityMol Imaging Biol31728839The same procedure was used for human post-mortem tissue (Folio Biosciences, Miami Brain Bank, and UCSF Memory and Aging Center, USA). In Vitro Homogenate Binding. All homogenate studies were carried out in 96-well format as previously described [14]Full Paper
2019Gao, L;Yuan, Z;Zhou, T;Yang, Y;Gao, D;Dunham, R;Liu, Z;FOXO genes in channel catfish and their response after bacterial infectionDev. Comp. Immunol.30905685; Plus Kit (Qiagen). RNA-seq library was prepared and sequenced by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) using an Illumina HiSeq 2000 instrument with 100 bp paired end (PE) reads. The trimmed highFull Paper
2019Havird, JC;Meyer, E;Fujita, Y;Vaught, RC;Henry, RP;Santos, SR;Disparate responses to salinity across species and organizational levels in anchialine shrimpsJ. Exp. Biol.31727759Assembly of reference transcriptomes was previously described for H. rubra (Havird and Santos, 2016a) and the four species from Japan (Havird et al., 2014b). Briefly, total RNA was extracted from whole animals 1 month after preservation in RNAlater vFull Paper
2019Rey, EG;Finkelstein, JL;Erickson, D;Fluorescence lateral flow competitive protein binding assay for the assessment of serum folate concentrationsPLoS ONE31166978Serum sample preparation. Calibration curves made with human serum spiked with FA and 5-MTHF were performed as part of a previous work[26]. Human serum samples of varying folate concentration were purchased from Discovery Life Sciences Inc. (Los OsosFull Paper
2019Mehnert, JM;Varga, A;Brose, MS;Aggarwal, RR;Lin, CC;Prawira, A;de Braud, F;Tamura, K;Doi, T;Piha-Paul, SA;Gilbert, J;Saraf, S;Thanigaimani, P;Cheng, JD;Keam, B;Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with advanced, PD-L1-positive papillary or follicular thyroid cancerBMC Cancer30832606biopsy sample. PD-L1 expression was assessed using a prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, California) [22] and the 22C3 antibody (Merck & Co., Inc., Kenilworth, New Jersey). PD ;screening period using eiFull Paper
2019Huang, RSP;Holmes, BF;Powell, C;Marati, RV;Tyree, D;Admire, B;Streator, A;Newell, AEH;Perez, J;Dalvi, D;ElGabry, EA;Delta-like Protein 3 Prevalence in Small Cell Lung Cancer and DLL3 (SP347) Assay CharacteristicsArch. Pathol. Lab. Med.30958693formalin), paraffin-embedded SCLC whole-section samples was procured from an internal tissue bank at Ventana Medical Systems, Inc, and the following commercial tissue banks: Asterand Bioscience; Avaden BioSciences; Boca Biolistics; Conversant Bio; GLFull Paper
2019Tsypin, M;Asmellash, S;Meyer, K;Touchet, B;Roder, H;Extending the information content of the MALDI analysis of biological fluids via multi-million shot analysisPLoS ONE31815946100 serum samples were purchased from the commercial biobanks Conversant Bio (Huntsville, AL) and Oncology Metrics (Fort Worth, TX). Samples were collected under ethics-approved protocols according to the requirements of Conversant Bio and Oncology MFull Paper
2019Li, S;Song, C;Yang, S;Yu, W;Zhang, W;Zhang, G;Xi, Z;Lu, E;Supercritical CO2 foamed composite scaffolds incorporating bioactive lipids promote vascularized bone regeneration via Hif-1α upregulation and enhanced type H vessel formationActa biomaterialia31154054Upon reaching 80-90% confluency, the rBMSCs were passaged with passages 2-4 being used for further experiments. HUVECs (AllCells, Shanghai, China) were cultured in endothelial cell medium (ECM, ScienCell, USA) under the same conditions and used for iFull Paper
2019Desai, SP;Coston, A;Berlin, A;Micro-Electrical Impedance Spectroscopy and Identification of Patient-Derived, Dissociated Tumor CellsIEEE Trans Nanobioscience31180894Stored waveforms are further analyzed using statistical data analysis techniques. C. Dissociated Tumor Cells and Cell Culture Dissociated tumor cells (DTCs) were acquired directly from Conversant Bio (Huntsville, AL) for a range of indications and caFull Paper
2019Lee, JS;Rosoff, D;Luo, A;Longley, M;Phillips, M;Charlet, K;Muench, C;Jung, J;Lohoff, FW;PCSK9 is Increased in Cerebrospinal Fluid of Individuals With Alcohol Use DisorderAlcohol. Clin. Exp. Res.30933362the NIH Institutional Review Board (IRB). All participants provided written informed consent before participation. CSF of deidentified controls was purchased from Discovery Life Sciences, Inc. (Research Biospecimen Sample Bank, Los Osos, CA, USA)Full Paper
2019Burbelo, PD;Chaturvedi, A;Notkins, AL;Gunti, S;Luciferase-Based Detection of Antibodies for the Diagnosis of HPV-Associated Head and Neck Squamous Cell CarcinomaDiagnostics (Basel)31390810The serum samples from colon cancer (CC), systemic lupus erythematosus (SLE), and HNSCC patients used in this report were obtained from Conversant Bio (601 Genome Way Suite 1200, Huntsville, Alabama 35806). SubjectsFull Paper
2019Kao, JH;Lin, CY;Chuang, WL;Cheng, YY;Hu, JY;Liang, WK;Friebe, P;Palmer, S;Huang, CS;Clinical evaluation of IntelliPlexTM HCV genotyping kit for hepatitis C virus genotypingDiagn. Microbiol. Infect. Dis.30975461as certified by the commercial supplier in the accompanying Certificate of Analysis (CoA)), including genotypes 3, 4 and 5 were spiked into the sample pool (samples were obtained from SeraCare Life Sciences Inc., [Cambridge, MA United States]; DiscovFull Paper
2019Lagatie, O;Verheyen, A;Nijs, E;Batsa Debrah, L;Debrah, YA;Stuyver, LJ;Performance evaluation of 3 serodiagnostic peptide epitopes and the derived multi-epitope peptide OvNMP-48 for detection of Onchocerca volvulus infectionParasitol. Res.31089811and informed consent, which was signed by all subjects. For samples obtained from Tissue Solutions Ltd. (Glasgow, Scotland), Discovery Life Sciences, Inc. (Los Osos, USA), Bioreclamation IVT (Baltimore, USA), Mayo ClinicFull Paper
2019Montazeri, M;Marbut, C;Huitink, D;Interconnect Fatigue Failure Parameter Isolation for Power Device Reliability Prediction in Alternative Accelerated Mechanical Cycling TestJournal of Electronic PackagingIEEE Forth Workshop on Wide Bandgap Power Devices and Applications ( WiPDA), Fayetteville, AR, Nov. 7-9, pp. 194– 199. 19. Qualitek, 2014, “Technical Data Sheet: Sn42/Bi58 Solder Wire,” Qualtek Electronics Corporation, Addison, IL, Accessed MaFull Paper
2019Mohan, S;Lawton, R;Palmer, C;Rojas, AC;Competitive ELISA method for novel estrogen-negative breast cancer biomarker quantitationJ. Immunol. Methods31533022system) (Rakha et al., 2008), and categorized based on estrogen-hormone-receptor orientation (viz., ER − versus ER + , n = 50), were obtained from cooperative human tissue network (Philadelphia, PA), Tissue-for-Research (Atlanta, GA), and ConversantFull Paper
2019Nicol, AF;de Andrade, CV;Gomes, SC;Brusadelli, MG;Lodin, HM;Wells, SI;Nuovo, GJ;The distribution of novel biomarkers in carcinoma-in-situ, microinvasive, and squamous cell carcinoma of the uterine cervixAnn Diagn Pathol30579259National Institute of Infectious Diseases Evandro Chagas - INI-Fiocruz, Rio de Janeiro, Brazil.++National Institute of Health of Women, Children, and Adolescents, Fernandes Figueira - IFF-FIOCRUZ, Rio de Janeiro, Brazil.++National Institute of HealthFull Paper
2019Mimitou, EP;Cheng, A;Montalbano, A;Hao, S;Stoeckius, M;Legut, M;Roush, T;Herrera, A;Papalexi, E;Ouyang, Z;Satija, R;Sanjana, NE;Koralov, SB;Smibert, P;Multiplexed detection of proteins, transcriptomes, clonotypes and CRISPR perturbations in single cellsNature methods31011186FBS 10% DMSO and aliquots of 1-1.5 million cells were thawed on the day of the experiment. PBMCs were obtained cryopreserved from AllCells (USA) and used immediately after thawing. NIH-3T3 and HEK293FT cells expressing non-targeting sgRNAs were maintFull Paper
2019Symeonides, S;Choice of Law in the American Courts in 2018: Thirty-Second Annual SurveyThe American Journal of Comparative LawIn re EP Floors Corp., No. 14-18-00610-CV, 2018 WL 4354688 (Tex. App. Sept. 13, 2018); In re Rosewood Private Invs., Inc., No. 05-18-00166-CV, 2018 WL 4403749 (Tex. App. Sept. 17, 2018); Bundy v. Adesa Houston D/B/A Adesa, Inc., No. 01-17-00863-CV, 2Full Paper
2019Yant, SR;Mulato, A;Hansen, D;Tse, WC;Niedziela-Majka, A;Zhang, JR;Stepan, GJ;Jin, D;Wong, MH;Perreira, JM;Singer, E;Papalia, GA;Hu, EY;Zheng, J;Lu, B;Schroeder, SD;Chou, K;Ahmadyar, S;Liclican, A;Yu, H;Novikov, N;Paoli, E;Gonik, D;Ram, RR;Hung, M;McDougall, WM;Brass, AL;Sundquist, WI;Cihlar, T;Link, JO;A highly potent long-acting small-molecule HIV-1 capsid inhibitor with efficacy in a humanized mouse modelNature medicine31501601Human PBMCs were collected from consenting healthy volunteers under informed consent; their use was approved by an institutional review board at AllCells. Human PBMCs, CD4 + T SaveFull Paper
2019Qu, L;Yi, Z;Zhu, S;Wang, C;Cao, Z;Zhou, Z;Yuan, P;Yu, Y;Tian, F;Liu, Z;Bao, Y;Zhao, Y;Wei, W;Programmable RNA editing by recruiting endogenous ADAR using engineered RNAsNature biotechnology3130854010% FBS and IL2 (1,000 U ml-1) and stimulated with CD3/CD28 DynaBeads (ThermoFisher, 11131D) for 2 d. Leukapheresis products from healthy donors were acquired from AllCells LLC China. All healthy donors provided informed consentFull Paper
2019Granja, JM;Klemm, S;McGinnis, LM;Kathiria, AS;Mezger, A;Corces, MR;Parks, B;Gars, E;Liedtke, M;Zheng, GXY;Chang, HY;Majeti, R;Greenleaf, WJ;Single-cell multiomic analysis identifies regulatory programs in mixed-phenotype acute leukemiaNature biotechnology31792411Description of healthy donors. PBMCs, BMMCs and CD34+ bone marrow cells were obtained from healthy donors with informed consent and compliance with relevant ethical regulations (AllCells). Individual information for each donor is provided in SupplemeFull Paper
2019Satpathy, AT;Granja, JM;Yost, KE;Qi, Y;Meschi, F;McDermott, GP;Olsen, BN;Mumbach, MR;Pierce, SE;Corces, MR;Shah, P;Bell, JC;Jhutty, D;Nemec, CM;Wang, J;Wang, L;Yin, Y;Giresi, PG;Chang, ALS;Zheng, GXY;Greenleaf, WJ;Chang, HY;Massively parallel single-cell chromatin landscapes of human immune cell development and intratumoral T cell exhaustionNature biotechnology31375813obtained from AllCells or the AllCells and isolated per manufacturer recommendations (as outlined in Supplementary Table 4). FACS-isolated populations were obtained from AllCells SaveFull Paper
2019Miller, JC;Patil, DP;Xia, DF;Paine, CB;Fauser, F;Richards, HW;Shivak, DA;Bendaña, YR;Hinkley, SJ;Scarlott, NA;Lam, SC;Reik, A;Zhou, Y;Paschon, DE;Li, P;Wangzor, T;Lee, G;Zhang, L;Rebar, EJ;Enhancing gene editing specificity by attenuating DNA cleavage kineticsNature biotechnology31359006CD34 + stem and progenitor cells were purified from mobilized peripheral blood leukapheresis products obtained from AllCells. Platelet depletion was performed, CD34 + cells were SaveFull Paper
2019Kinney, MA;Vo, LT;Frame, JM;Barragan, J;Conway, AJ;Li, S;Wong, KK;Collins, JJ;Cahan, P;North, TE;Lauffenburger, DA;Daley, GQ;A systems biology pipeline identifies regulatory networks for stem cell engineeringNature biotechnology31267104Human CD34 + progenitors derived from mobilized peripheral blood (AllCells) were expanded for 4 d in StemSpan SFEM (StemCell Technologies) with the addition of interleukin (IL)-3 ( SaveFull Paper
2019Lareau, CA;Duarte, FM;Chew, JG;Kartha, VK;Burkett, ZD;Kohlway, AS;Pokholok, D;Aryee, MJ;Steemers, FJ;Lebofsky, R;Buenrostro, JD;Droplet-based combinatorial indexing for massive-scale single-cell chromatin accessibilityNature biotechnology31235917CD34+ stem/progenitor cells, peripheral blood mononuclear cells (PBMC), and isolated peripheral blood CD4+, CD8+, CD14+, CD19+ and CD56+ cells were purchased from Allcells ( Save;BMMCs, isolated bone marrow CD34 + stem/progenitor cells, PBMCs, and iFull Paper
2019Setty, M;Kiseliovas, V;Levine, J;Gayoso, A;Mazutis, L;Pe'er, D;Characterization of cell fate probabilities in single-cell data with PalantirNature biotechnology30899105incorporate lineage information to model cell fate decisions. scRNA-seq of CD34+ human bone marrow cells Cryopreserved bone marrow stem/progenitor CD34+ cells from healthy donors were purchased from AllCells, LLC. (Cat. No. ABM022F) and stored in vaFull Paper
2019Shah, NJ;Mao, AS;Shih, TY;Kerr, MD;Sharda, A;Raimondo, TM;Weaver, JC;Vrbanac, VD;Deruaz, M;Tager, AM;Mooney, DJ;Scadden, DT;An injectable bone marrow-like scaffold enhances T cell immunity after hematopoietic stem cell transplantationNature biotechnology30742125t-specific antibodies. We isolated hematopoietic cells (Lin−Sca-1hic-kithi) using a FacsAria cell sorter (BD). Sorted cells were ≥ 95% pure. Human cord-blood derived CD34+ cells were purchased (Allcells) and expanded for seven days using expansion suFull Paper
2019Maun, HR;Jackman, JK;Choy, DF;Loyet, KM;Staton, TL;Jia, G;Dressen, A;Hackney, JA;Bremer, M;Walters, BT;Vij, R;Chen, X;Trivedi, NN;Morando, A;Lipari, MT;Franke, Y;Wu, X;Zhang, J;Liu, J;Wu, P;Chang, D;Orozco, LD;Christensen, E;Wong, M;Corpuz, R;Hang, JQ;Lutman, J;Sukumaran, S;Wu, Y;Ubhayakar, S;Liang, X;Schwartz, LB;Babina, M;Woodruff, PG;Fahy, JV;Ahuja, R;Caughey, GH;Kusi, A;Dennis, MS;Eigenbrot, C;Kirchhofer, D;Austin, CD;Wu, LC;Koerber, JT;Lee, WP;Yaspan, BL;Alatsis, KR;Arron, JR;Lazarus, RA;Yi, T;An Allosteric Anti-tryptase Antibody for the Treatment of Mast Cell-Mediated Severe AsthmaCell31585081Enriched CD34+ human cord blood stem cells were obtained from AllCells with more than 80% purity validated by flow cytometry analysis. CHO DKO cell line (Macaraeg et al., 2013) was a derivative of the CHO-K1 cell line (ATCC number CCL-61) was grown aFull Paper
2019Stuart, T;Butler, A;Hoffman, P;Hafemeister, C;Papalexi, E;Mauck, WM;Hao, Y;Stoeckius, M;Smibert, P;Satija, R;Comprehensive Integration of Single-Cell DataCell31178118Hashtag 9 Cell Hashing antibody BioLegend Cat# 394617 Hashtag 10 Cell Hashing antibody BioLegend Cat# 394619 Biological Samples Human bone marrow cells AllCells Cat# ABM007F, lot 3008803 Critical Commercial Assays 3′ scRNA-seq kit 10x Genomics V2 cheFull Paper
2019Abelin, JG;Harjanto, D;Malloy, M;Suri, P;Colson, T;Goulding, SP;Creech, AL;Serrano, LR;Nasir, G;Nasrullah, Y;McGann, CD;Velez, D;Ting, YS;Poran, A;Rothenberg, DA;Chhangawala, S;Rubinsteyn, A;Hammerbacher, J;Gaynor, RB;Fritsch, EF;Greshock, J;Oslund, RC;Barthelme, D;Addona, TA;Arieta, CM;Rooney, MS;Defining HLA-II Ligand Processing and Binding Rules with Mass Spectrometry Enhances Cancer Epitope PredictionImmunity31495665Human Healthy Donor Leukapheresis Material AllCells NA Human Healthy Donor Leukapheresis Material PPA Research NA Human Healthy Donor Leukapheresis Material StemExpress NA Human Healthy Donor Leukapheresis Material StemCell Technologies NAFull Paper
2019Lindner, JM;Cornacchione, V;Sathe, A;Be, C;Srinivas, H;Riquet, E;Leber, XC;Hein, A;Wrobel, MB;Scharenberg, M;Pietzonka, T;Wiesmann, C;Abend, J;Traggiai, E;Human Memory B Cells Harbor Diverse Cross-Neutralizing Antibodies against BK and JC PolyomavirusesImmunity30824324Purified peripheral blood mononuclear cells from leukapharesis AllCells, Inc. N/A leukapharesis and matched serum samples were obtained from AllCells LLC (Alameda, CA, USA). SaveFull Paper
2019Li, R;Wang, Y;Zhang, X;Feng, M;Ma, J;Li, J;Yang, X;Fang, F;Xia, Q;Zhang, Z;Shang, M;Jiang, S;Exosome-mediated secretion of LOXL4 promotes hepatocellular carcinoma cell invasion and metastasisMolecular cancer30704479HUVECs were purchased from ATCC and cultivated in endothelial cell complete medium containing endothelial cell growth supplement (Allcells, USA). For hypoxic culture, HCC cells SaveFull Paper
2019Jiang, Q;Isquith, J;Zipeto, MA;Diep, RH;Pham, J;Delos Santos, N;Reynoso, E;Chau, J;Leu, H;Lazzari, E;Melese, E;Ma, W;Fang, R;Minden, M;Morris, S;Ren, B;Pineda, G;Holm, F;Jamieson, C;Hyper-Editing of Cell-Cycle Regulatory and Tumor Suppressor RNA Promotes Malignant Progenitor PropagationCancer cell30612940Institutional Review Board approved protocol (#131550) that meets the requirements as stated in 45 CFR 46.404 and 21 CFR 50.51. De-identified (IRB exempt) human cord blood and normal aged-match samples were purchased as purified CD34+ cells from AllCFull Paper
2019Adelman, ER;Huang, HT;Roisman, A;Olsson, A;Colaprico, A;Qin, T;Lindsley, RC;Bejar, R;Salomonis, N;Grimes, HL;Figueroa, ME;Aging Human Hematopoietic Stem Cells Manifest Profound Epigenetic Reprogramming of Enhancers That May Predispose to LeukemiaCancer discovery31085557AML, which is more frequent in the elderly, is characterized by epigenetic deregulation. We demonstrate that epigenetic reprogramming of human HSCs occurs with age, affecting SaveFull Paper
2019Gong, X;Du, J;Parsons, SH;Merzoug, FF;Webster, Y;Iversen, PW;Chio, LC;Van Horn, RD;Lin, X;Blosser, W;Han, B;Jin, S;Yao, S;Bian, H;Ficklin, C;Fan, L;Kapoor, A;Antonysamy, S;Mc Nulty, AM;Froning, K;Manglicmot, D;Pustilnik, A;Weichert, K;Wasserman, SR;Dowless, M;Marugán, C;Baquero, C;Lallena, MJ;Eastman, SW;Hui, YH;Dieter, MZ;Doman, T;Chu, S;Qian, HR;Ye, XS;Barda, DA;Plowman, GD;Reinhard, C;Campbell, RM;Henry, JR;Buchanan, SG;Aurora A Kinase Inhibition Is Synthetic Lethal with Loss of the RB1 Tumor Suppressor GeneCancer discovery30373917The identification of a synthetic lethal interaction between RB1 and AURKA inhibition, and the discovery of a drug that can be dosed continuously to achieve uninterrupted inhibition of SaveFull Paper
2019Mascarenhas, J;Lu, M;Kosiorek, H;Virtgaym, E;Xia, L;Sandy, L;Mesa, R;Petersen, B;Farnoud, N;Najfeld, V;Rampal, R;Dueck, A;Hoffman, R;Oral idasanutlin in patients with polycythemia veraBlood31167802Baseline MDM2 protein levels in the enrolled PV/ET patients and 7 normal samples (peripheral blood, n = 4; bone marrow, n = 3) purchased from ALLcells (Alameda, CA) were evaluated using monoclonal antibody staining and flow cytometric analysis.24 BriFull Paper
2019Drissen, R;Thongjuea, S;Theilgaard-Mönch, K;Nerlov, C;Identification of two distinct pathways of human myelopoiesisScience immunology31126997Bone marrow samples were from AllCells or taken from healthy male volunteers at the age of 21 to 29 years old, who provided written informed consent in accordance with local SaveFull Paper
2019Hu, CW;Qiu, Y;Ligeralde, A;Raybon, AY;Yoo, SY;Coombes, KR;Qutub, AA;Kornblau, SM;A quantitative analysis of heterogeneities and hallmarks in acute myelogenous leukaemiaNature biomedical engineering30988472was prepared as described in publications11,21. In addition to AML patient samples, each array includes the same ten cryopreserved normal bone marrow CD34+ samples (NLBM CD34+) from healthy subjects (AllCells, Alameda, CA). Mutation Analysis All mutFull Paper
2019Maekawa, T;Kato, S;Kawamura, T;Takada, K;Sone, T;Ogata, H;Saito, K;Izumi, T;Nagao, S;Takano, K;Okada, Y;Tachi, N;Teramoto, M;Horiuchi, T;Hikota-Saga, R;Endo-Umeda, K;Uno, S;Osawa, Y;Kobayashi, A;Kobayashi, S;Sato, K;Hashimoto, M;Suzu, S;Usuki, K;Morishita, S;Araki, M;Makishima, M;Komatsu, N;Kimura, F;Increased SLAMF7high monocytes in myelofibrosis patients harboring JAK2V617F provide a therapeutic target of elotuzumabBlood31270105The hNOG mouse was created by transplanting human cord blood (Allcells, Alameda, CA) into the NOG mouse established by backcrossing an interleukin-2R (IL-2R) γ-chain knockout SaveFull Paper
2019Pang, WW;Czechowicz, A;Logan, AC;Bhardwaj, R;Poyser, J;Park, CY;Weissman, IL;Shizuru, JA;Anti-CD117 antibody depletes normal and myelodysplastic syndrome human hematopoietic stem cells in xenografted miceBlood30745302Normal human BM samples were purchased from AllCells (Alameda, CA). Human umbilical cord blood (UCB) samples and human BM samples from MDS patients were obtained SaveFull Paper
2019Abdel-Azim, H;Dvorak, C;Bunin, N;Walters, M;Talano, J;Anderson, E;Malvar, J;Al Jassani, M;Chaudhury, S;Cairo, M;Kitko, C;Jacob, A;Monos, D;Wing, L;Pulsipher, M;Next Generation Sequence Minimal Residual Disease (NGS-MRD) Predicts Outstanding Event Free Survival (EFS) Regardless of Hematopoietic Cell Transplantation (HCT) Preparative Approach or Graft Alpha/Beta Depletion in Children with Acute Lymphoblastic Leukemia (ALL)BloodMRD detected by flow cytometry (FC) or PCR has been associated with key outcomes after HCT for ALL. In a prospective multicenter trial (NCT02646839; Pediatric Blood and Marrow Transplant Consortium [PBMTC] ONC1401), we performed a planned sub-study oFull Paper
2019Dunbar, A;Kosiorek, H;Krishnan, A;McGovern, E;Park, Y;Patel, M;Weinberg, R;Yacoub, A;Dueck, A;Mascarenhas, J;Levine, R;Hoffman, R;Rampal, R;Identifying Cytokine Biomarkers of Response to Pegylated-Interferon Therapy in Polycythemia Vera and Essential Thrombocythemia: Correlative Analysis from the MPN-RC 111/112 TrialsBloodAge-matched serum from healthy individuals (N=6) was obtained from AllCells TM for use as wild-type (WT) controls. Baseline cytokine samples were compared between groups of Save;Background: Pegylated Interferon-alpha (PEG-IFNa) improves hematologicFull Paper
2019Park, SH;Lee, CM;Dever, DP;Davis, TH;Camarena, J;Srifa, W;Zhang, Y;Paikari, A;Chang, AK;Porteus, MH;Sheehan, VA;Bao, G;Highly efficient editing of the β-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell diseaseNucleic acids research31147717K562 cells were cultured in RPMI1640 (ATCC, Cat No. 30-2001) supplemented with 10% FBS and L-glutamine. Frozen CD34+ cells from mobilized peripheral blood of normal individuals were purchased from AllCells. Peripheral blood CD34+ cells were acquiredFull Paper
2019Shortridge, MD;Wille, PT;Jones, AN;Davidson, A;Bogdanovic, J;Arts, E;Karn, J;Robinson, JA;Varani, G;An ultra-high affinity ligand of HIV-1 TAR reveals the RNA structure recognized by P-TEFbNucleic acids research30481318stimulate HIV-1 transcription. Twenty-four hours later, the percentage of cells expressing GFP was determined by flow cytometry as described above. Peptide uptake Frozen, de-identified human PBMCs (Allcells, Alameda, CA) were thawed and stimulated wFull Paper
2019McGrath, E;Shin, H;Zhang, L;Phue, JN;Wu, WW;Shen, RF;Jang, YY;Revollo, J;Ye, Z;Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencingNature communications31767844Briefly, CD34+ cells from a healthy male donor were purchased from AllCells (Alameda, CA, USA) and were cultured in StemSpan medium (STEMCELL Technologies, Vancouver, BC, SaveFull Paper
2019Sachdeva, M;Busser, BW;Temburni, S;Jahangiri, B;Gautron, AS;Maréchal, A;Juillerat, A;Williams, A;Depil, S;Duchateau, P;Poirot, L;Valton, J;Repurposing endogenous immune pathways to tailor and control chimeric antigen receptor T cell functionalityNature communications31723132are able to integrate complex environmental inputs and to generate custom outputs in a highly regulated and specific manner. Methods Biological materials Cryopreserved human PBMCs were acquired from ALLCELLS (cat # PB006F) and used in accordance witFull Paper
2019Gomez-Ospina, N;Scharenberg, SG;Mostrel, N;Bak, RO;Mantri, S;Quadros, RM;Gurumurthy, CB;Lee, C;Bao, G;Suarez, CJ;Khan, S;Sawamoto, K;Tomatsu, S;Raj, N;Attardi, LD;Aurelian, L;Porteus, MH;Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type INature communications31492863ck-treated samples, and 50% modification control were included. Data was analyzed using QuantaSoft (Bio-Rad). HSPC selection and culturing Human CD34+ HSPCs mobilized peripheral blood purchased from AllCells (Alameda, CA, USA) and thawed per manufacFull Paper
2019Zhang, Y;Roos, M;Himburg, H;Termini, CM;Quarmyne, M;Li, M;Zhao, L;Kan, J;Fang, T;Yan, X;Pohl, K;Diers, E;Jin Gim, H;Damoiseaux, R;Whitelegge, J;McBride, W;Jung, ME;Chute, JP;PTPσ inhibitors promote hematopoietic stem cell regenerationNature communications31413255collected from the recipient mice and were analyzed for percentages of DsRed+ cells in the BM17. Human BM cultures and human BM transplantation assays Human BM mononuclear cells were purchased from AllCells. Cryopreserved human BM cells were recoverFull Paper
2019Pellin, D;Loperfido, M;Baricordi, C;Wolock, SL;Montepeloso, A;Weinberg, OK;Biffi, A;Klein, AM;Biasco, L;A comprehensive single cell transcriptional landscape of human hematopoietic progenitorsNature communications31160568from MNCs with the human anti-CD34 MicroBeads Isolation Kit (Miltenyi Biotec) according to the manufacturer's specifications or were purchased from commercial sources (AllCells). SaveFull Paper
2019Pavel-Dinu, M;Wiebking, V;Dejene, BT;Srifa, W;Mantri, S;Nicolas, CE;Lee, C;Bao, G;Kildebeck, EJ;Punjya, N;Sindhu, C;Inlay, MA;Saxena, N;DeRavin, SS;Malech, H;Roncarolo, MG;Weinberg, KI;Porteus, MH;Gene correction for SCID-X1 in long-term hematopoietic stem cellsNature communications30967552d to the purified AAV6 at a final concentration of 0.001%, aliquot and stored at −80 °C. CD34+ HSPCs Mobilized peripheral blood  (mPB) and bone marow (BM) CD34+ HSPCs cells were purchased from AllCells (Alameda, CA, USA). Cells were thawed using pubFull Paper
2019Kimmey, SC;Borges, L;Baskar, R;Bendall, SC;Parallel analysis of tri-molecular biosynthesis with cell identity and function in single cellsNature communications30862852Fresh bone marrow aspirates from two independent donors were obtained from AllCells (Alameda, CA) on the same day of collection, and immediately after receiving was placed in 37 SaveFull Paper
2019Paschon, DE;Lussier, S;Wangzor, T;Xia, DF;Li, PW;Hinkley, SJ;Scarlott, NA;Lam, SC;Waite, AJ;Truong, LN;Gandhi, N;Kadam, BN;Patil, DP;Shivak, DA;Lee, GK;Holmes, MC;Zhang, L;Miller, JC;Rebar, EJ;Diversifying the structure of zinc finger nucleases for high-precision genome editingNature communications30850604rer’s instructions. Target loci were then analyzed by PCR amplification followed by deep sequencing (MiSeq). Studies performed in T-cells Peripheral blood CD3 + Pan T-cells were purchased from AllCells (Alameda, CA). Transfections into activated T-cFull Paper
2019Czechowicz, A;Palchaudhuri, R;Scheck, A;Hu, Y;Hoggatt, J;Saez, B;Pang, WW;Mansour, MK;Tate, TA;Chan, YY;Walck, E;Wernig, G;Shizuru, JA;Winau, F;Scadden, DT;Rossi, DJ;Selective hematopoietic stem cell ablation using CD117-antibody-drug-conjugates enables safe and effective transplantation with immunity preservationNature communications30728354als into 9 Gy, lethally irradiated, C57BL/6J (CD45.2+) wild-type recipients as previously described16. Human cord blood transplantation Single donor-derived cord blood CD34+ cells were obtained from AllCells (Alameda, CA) under local IRB-approved prFull Paper
2019Zeng, Y;Yao, X;Liu, X;He, X;Li, L;Liu, X;Yan, Z;Wu, J;Fu, BM;Anti-angiogenesis triggers exosomes release from endothelial cells to promote tumor vasculogenesisJournal of extracellular vesicles31258881o inhibit tumor vasculogenesis may serve as a novel strategy to augment the long-term efficacy of anti-angiogenic and anti-autophagic therapies for tumors. Materials and methods Cell culture HUVECs (Allcells, Shanghai, China) were cultured in HUVECFull Paper
2019Mu, Q;Cabana-Puig, X;Mao, J;Swartwout, B;Abdelhamid, L;Cecere, TE;Wang, H;Reilly, CM;Luo, XM;Pregnancy and lactation interfere with the response of autoimmunity to modulation of gut microbiotaMicrobiome31311609Human plasma samples were purchased from AllCells (Alameda, CA). For measuring anti-DNA IgG, anti-LPS IgG, and anti-flagellin IgG, high-binding plates were coated overnight at 4 SaveFull Paper
2019Li, W;Wu, D;Zhu, S;Liu, Z;Luo, B;Lu, L;Zhou, C;Sustained release of plasmid DNA from PLLA/POSS nanofibers for angiogenic therapyChemical Engineering JournalHuman umbilical vein endothelial cells (HUVECs, purchased from Allcells, shanghai, China) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (P/S, Gibco) at 37 °C in 5% CO2Full Paper
2019Wu, HW;Sheard, MA;Malvar, J;Fernandez, GE;DeClerck, YA;Blavier, L;Shimada, H;Theuer, CP;Sposto, R;Seeger, RC;Anti-CD105 Antibody Eliminates Tumor Microenvironment Cells and Enhances Anti-GD2 Antibody Immunotherapy of Neuroblastoma with Activated Natural Killer CellsClinical cancer research : an official journal of the American Association for Cancer Research31068371Human umbilical vein endothelial cells (HUVEC) were purchased from AllCells, LLC and maintained in HUVEC Basal Medium containing 10% Stimulatory Supplement from AllCells in SaveFull Paper
2019Zuch de Zafra, CL;Fajardo, F;Zhong, W;Bernett, MJ;Muchhal, US;Moore, GL;Stevens, J;Case, R;Pearson, JT;Liu, S;McElroy, PL;Canon, J;Desjarlais, JR;Coxon, A;Balazs, M;Nolan-Stevaux, O;Targeting Multiple Myeloma with AMG 424, a Novel Anti-CD38/CD3 Bispecific T-cell-recruiting Antibody Optimized for Cytotoxicity and Cytokine ReleaseClinical cancer research : an official journal of the American Association for Cancer Research30918018Frozen human T cells (AllCells) or frozen peripheral blood mononuclear cells (PBMC) from humans or cynomolgus (CepheusBio) were cocultured with target cells at an effector cell (E) SaveFull Paper
2019Jin, Q;Martinez, CA;Arcipowski, KM;Zhu, Y;Gutierrez-Diaz, BT;Wang, KK;Johnson, MR;Volk, AG;Wang, F;Wu, J;Grove, C;Wang, H;Sokirniy, I;Thomas, PM;Goo, YA;Abshiru, NA;Hijiya, N;Peirs, S;Vandamme, N;Berx, G;Goosens, S;Marshall, SA;Rendleman, EJ;Takahashi, YH;Wang, L;Rawat, R;Bartom, ET;Collings, CK;Van Vlierberghe, P;Strikoudis, A;Kelly, S;Ueberheide, B;Mantis, C;Kandela, I;Bourquin, JP;Bornhauser, B;Serafin, V;Bresolin, S;Paganin, M;Accordi, B;Basso, G;Kelleher, NL;Weinstock, J;Kumar, S;Crispino, JD;Shilatifard, A;Ntziachristos, P;USP7 Cooperates with NOTCH1 to Drive the Oncogenic Transcriptional Program in T-Cell LeukemiaClinical cancer research : an official journal of the American Association for Cancer Research30224337were maintained in DMEM medium supplemented with 10% heat-inactivated FBS, 2% penicillin/streptomycin, and 1% GlutaMAX. Human pan T cells were purchased from AllCells.com (Alameda, CA). Primary human samples were collected by collaborating institutioFull Paper
2019Zheng, B;Yu, SF;Del Rosario, G;Leong, SR;Lee, GY;Vij, R;Chiu, C;Liang, WC;Wu, Y;Chalouni, C;Sadowsky, J;Clark, V;Hendricks, A;Poon, KA;Chu, W;Pillow, T;Schutten, MM;Flygare, J;Polson, AG;An Anti-CLL-1 Antibody-Drug Conjugate for the Treatment of Acute Myeloid LeukemiaClinical cancer research : an official journal of the American Association for Cancer Research29959143Bone marrow mononuclear cells (BM-MNC) from healthy human donors (AllCells) were prepared by density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare). CD34 + SaveFull Paper
2019Castillo, J;Wu, E;Lowe, C;Srinivasan, S;McCord, R;Wagle, MC;Jayakar, S;Edick, MG;Eastham-Anderson, J;Liu, B;Hutchinson, KE;Jones, W;Stokes, MP;Tarighat, SS;Holcomb, T;Glibicky, A;Romero, FA;Magnuson, S;Huang, SA;Plaks, V;Giltnane, JM;Lackner, MR;Mounir, Z;CBP/p300 Drives the Differentiation of Regulatory T Cells through Transcriptional and Non-Transcriptional MechanismsCancer research31182547Normal human peripheral blood CD4+/CD45RA+/CD25− naïve T cells were procured from Allcells (PB009-5). Cells were isolated using Immunomagnetic CD4+ Isolation Kit, CD45RO microbeads to deplete CD45RO+ cells, and cultured using Dynabeads human T activaFull Paper
2019Levi, J;Lam, T;Goth, SR;Yaghoubi, S;Bates, J;Ren, G;Jivan, S;Huynh, TL;Blecha, JE;Khattri, R;Schmidt, KF;Jennings, D;VanBrocklin, H;Imaging of Activated T Cells as an Early Predictor of Immune Response to Anti-PD-1 TherapyCancer research31064845Neutrophils and eosinophils were isolated from human whole blood (AllCells, Alameda CA). Human neutrophil and eosinophil enrichment kit (StemCell Technologies) was used to purify the cells. Neutrophils were either treated with 1uM N-formyl- L-methionFull Paper
2019Boudreaux, SP;Duren, RP;Call, SG;Nguyen, L;Freire, PR;Narayanan, P;Redell, MS;Conneely, OM;Drug targeting of NR4A nuclear receptors for treatment of acute myeloid leukemiaLeukemia29884904rmed consent at Texas Children’s Hospital in accordance with the Declaration of Helsinki and institutional review board regulations. Normal cord blood CD34+ stem/progenitor cells were obtained from AllCells. Connectivity Map (CMAP) Analysis The NR4AFull Paper
2019Shen, JC;Kamath-Loeb, AS;Kohrn, BF;Loeb, KR;Preston, BD;Loeb, LA;A high-resolution landscape of mutations in the BCL6 super-enhancer in normal human B cellsProceedings of the National Academy of Sciences of the United States of America31748270Human B Cells. The CD19+ B cells were purchased from AllCells and ReachBio. Peripheral blood mononuclear cells (PBMCs) were purified from whole blood drawn from 10 healthy donors (SI Appendix, Table S2). CD19+ B cells were purified by positive selectFull Paper
2019Zhang, L;Dong, X;Lee, M;Maslov, AY;Wang, T;Vijg, J;Single-cell whole-genome sequencing reveals the functional landscape of somatic mutations in B lymphocytes across the human lifespanProceedings of the National Academy of Sciences of the United States of America30992375Two frozen human umbilical cord blood mononuclear cell samples were obtained from AllCells and STEMCELL Technologies. Informed consent was obtained from all the donors. The SaveFull Paper
2019Ye, S;Cohen, D;Belmar, NA;Choi, D;Tan, SS;Sho, M;Akamatsu, Y;Kim, H;Iyer, R;Cabel, J;Lake, M;Song, D;Harlan, J;Zhang, C;Fang, Y;Wahl, AF;Culp, P;Hollenbaugh, D;Chao, DT;A Bispecific Molecule Targeting CD40 and Tumor Antigen Mesothelin Enhances Tumor-Specific ImmunityCancer immunology research31462409than 10 passages from master cell banking. Fresh human peripheral blood mononuclear cells (PBMC) from healthy donors (about 109 per donor) were purchased from AllCells. Donor virus testing results of human immunodeficiency virus, hepatitis B virus, aFull Paper
2019Gui, X;Deng, M;Song, H;Chen, Y;Xie, J;Li, Z;He, L;Huang, F;Xu, Y;Anami, Y;Yu, H;Yu, C;Li, L;Yuan, Z;Xu, X;Wang, Q;Chai, Y;Huang, T;Shi, Y;Tsuchikama, K;Liao, XC;Xia, N;Gao, GF;Zhang, N;Zhang, CC;An, Z;Disrupting LILRB4/APOE Interaction by an Efficacious Humanized Antibody Reverses T-cell Suppression and Blocks AML DevelopmentCancer immunology research31213474In the coculture assay, human T cells (5 × 10 4 cells per well) isolated from health donor peripheral blood (AllCells) were placed in the lower chambers of a 96-well transwell plate. SaveFull Paper
2019Newman, M;Abstract B178: Development and preclinical efficacy characterization of a systemically administered multiple Toll-like receptor (TLR) agonist for antitumor immunotherapyCancer Immunology ResearchPBMCs from fresh normal peripheral blood leukapheresis paks (ALLCells, Alameda, CA) were isolated using a Ficoll gradient. Ten-fold increments of 1x10/mL to 1x10^8/mL untreated SaveFull Paper
2019Sun, Y;Xiao, Y;Sun, H;Zhao, Z;Zhu, J;Zhang, L;Dong, J;Han, T;Jing, Q;Zhou, J;Jing, Z;miR-27a regulates vascular remodeling by targeting endothelial cells' apoptosis and interaction with vascular smooth muscle cells in aortic dissectionTheranostics31695809Prime HUVECs were purchased from ALLCELLS and cultured in endothelial cell media (ALLCELLS, Emeryville, California) at 37℃ in a humidified atmosphere with 5% CO 2 . HEK 293 SaveFull Paper
2019Varricchio, L;Planutis, A;Manwani, D;Jaffray, J;Mitchell, WB;Migliaccio, AR;Bieker, JJ;Genetic disarray follows mutant KLF1-E325K expression in a congenital dyserythropoietic anemia patientHaematologica30872368no new patient material was obtained for the present study. Mononuclear cells from the peripheral blood (PBMC) had been isolated and cryopreserved as described.35 Non-patient PBMC were purchased from AllCells (PB003F). Human erythroid massive amplifFull Paper
2019Sapienza, MR;Abate, F;Melle, F;Orecchioni, S;Fuligni, F;Etebari, M;Tabanelli, V;Laginestra, MA;Pileri, A;Motta, G;Rossi, M;Agostinelli, C;Sabattini, E;Pimpinelli, N;Truni, M;Falini, B;Cerroni, L;Talarico, G;Piccioni, R;Amente, S;Indio, V;Tarantino, G;Brundu, F;Paulli, M;Berti, E;Facchetti, F;Dellino, GI;Bertolini, F;Tripodo, C;Rabadan, R;Pileri, SA;Blastic plasmacytoid dendritic cell neoplasm: genomics mark epigenetic dysregulation as a primary therapeutic targetHaematologica30381297RNA of 4 normal plasmacytoid dendritic cell (pDCs) samples was purchased from AllCells (Alameda, CA, US) and used for comparison. For details, see Online Supplementary Table S6 Save;BPDCN cryopreserved cutaneous biopsies, sufficient only for RNA seFull Paper
2019Yao, X;Liu, R;Liang, X;Ding, J;Critical Areas of Proliferation of Single Cells on Micropatterned Surfaces and Corresponding Cell Type DependenceACS applied materials & interfaces30964630Primary rat MSCs were purchased from Allcells Tech., Shanghai, People’s Republic of China; primary mouse embryo fibroblasts (EF) were purchased from Shanghai Gaining Biological Technology Co., Ltd.; human cervix epithelial carcinoma cells (Hela) andFull Paper
2019Deng, X;Chu, X;Wang, P;Ma, X;Wei, C;Sun, C;Yang, J;Li, Y;MicroRNA-29a-3p Reduces TNFα-Induced Endothelial Dysfunction by Targeting Tumor Necrosis Factor Receptor 1Molecular therapy. Nucleic acids31760375ina), and HEK293 cells (ATCC, Manassas, VA, USA) were maintained in DMEM (HyClone, South Logan, UT, USA) containing 10% fetal bovine serum (FBS; PAA Laboratories, Austria). pHUVECs were obtained from AllCells (China) and cultured incomplete medium (AFull Paper
2019Mandal, S;Prathipati, PK;Belshan, M;Destache, CJ;A potential long-acting bictegravir loaded nano-drug delivery system for HIV-1 infection: A proof-of-concept studyAntiviral research30991088TZM-bl cells were obtained from NIH AIDS reagent program (Kappes and Wu, 2018; Kappes et al., 2015). Peripheral blood mononuclear cells (PBMCs) were obtained from commercial vendor (AllCells Inc. Alameda, CA). TZM-bl cells were maintained in completeFull Paper
2019Bongen, E;Lucian, H;Khatri, A;Fragiadakis, GK;Bjornson, ZB;Nolan, GP;Utz, PJ;Khatri, P;Sex Differences in the Blood Transcriptome Identify Robust Changes in Immune Cell Proportions with Aging and Influenza InfectionCell reports31722210Sample preparation and CyTOF methods have been described previously (Fragiadakis et al., 2019). Briefly, peripheral blood was obtained from 86 human adult volunteers from the Stanford Blood Center, AllCells (exempt, non-human subjects research), andFull Paper
2019Shehata, L;Maurer, DP;Wec, AZ;Lilov, A;Champney, E;Sun, T;Archambault, K;Burnina, I;Lynaugh, H;Zhi, X;Xu, Y;Walker, LM;Affinity Maturation Enhances Antibody Specificity but Compromises Conformational StabilityCell reports31553901Biological Samples Healthy donor bone marrow mononuclear cells AllCells https://www.allcells.com/ Healthy human peripheral blood mononuclear cells Dartmouth Hitchcock Medical Center N/AFull Paper
2019Shi, D;Dong, Y;Dai, P;Gao, J;Yin, J;Xie, M;Dentin matrix protein 1 correlates with the severity of hemorrhagic fever with renal syndrome and promotes hyper-permeability of endothelial cells infected by Hantaan virusMicrobes and infection30735719Cells were cultured in AllCells complete endothelial cell medium and incubated at 37 C with 5% CO 2 . HUVECs (passage 3 or 4) of approximately 70-80% confluence were used for all SaveFull Paper
2019Muñoz, DP;Yannone, SM;Daemen, A;Sun, Y;Vakar-Lopez, F;Kawahara, M;Freund, AM;Rodier, F;Wu, JD;Desprez, PY;Raulet, DH;Nelson, PS;van 't Veer, LJ;Campisi, J;Coppé, JP;Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to agingJCI insight31184599Freshly isolated human PBMCs were maintained in supplemented RPMI media and cultured in 3% O2 conditions. Monocytes were obtained from ATCC (U934 and Thp1) or AllCells (CD36 selection; 99%pure). Purified NK, T, and DC cells were from AllCells (99%purFull Paper
2019Morgan, H;Tseng, SY;Gallais, Y;Leineweber, M;Buchmann, P;Riccardi, S;Nabhan, M;Lo, J;Gani, Z;Szely, N;Zhu, CS;Yang, M;Kiessling, A;Vohr, HW;Pallardy, M;Aswad, F;Turbica, I;Evaluation of in vitro Assays to Assess the Modulation of Dendritic Cells Functions by Therapeutic Antibodies and AggregatesFrontiers in immunology31001248Wuppertal (Germany). For each site, healthy donors gave their written consent for the use of blood donation for research purposes. Also, PBMC directly isolated and frozen were purchased from vendor (AllCells, CA, USA). In each partner lab's, PBMC isFull Paper
2019Saul, L;Mair, I;Ivens, A;Brown, P;Samuel, K;Campbell, JDM;Soong, DY;Kamenjarin, N;Mellanby, RJ;1,25-Dihydroxyvitamin D3 Restrains CD4+ T Cell Priming Ability of CD11c+ Dendritic Cells by Upregulating Expression of CD31Frontiers in immunology30984180SA as per manufacturer's instructions (eBioscience, San Diego, USA). Generation of Human DC From Mobilized Peripheral Blood CD34+ Progenitor Cells CD34+ cells were obtained from a commercial source (AllCells, LLC., Alameda, CA, USA). The CD34+ cellsFull Paper
2019Smith, AJ;Miller, SM;Buhl, C;Child, R;Whitacre, M;Schoener, R;Ettenger, G;Burkhart, D;Ryter, K;Evans, JT;Species-Specific Structural Requirements of Alpha-Branched Trehalose Diester Mincle AgonistsFrontiers in immunology30873180PBMCs were obtained via leukapheresis from normal donors (AllCells, Berkeley, CA) or fresh blood was obtained from health human donors through a University of Montana SaveFull Paper
2019Du, Y;Montoya, C;Orrego, S;Wei, X;Ling, J;Lelkes, PI;Yang, M;Topographic cues of a novel bilayered scaffold modulate dental pulp stem cells differentiation by regulating YAP signalling through cytoskeleton adjustmentsCell proliferation31424140ilometer (Surfcorder SE 1700, Kosaka Labs) with a 2 μm radius tip, a spread of 2.5 mm and a speed of 0.5 mm/s. 2.2 Dental pulp stem cells culture on the scaffolds Human DPSCs were purchased from AllCells LLC. These cells were guaranteed by company tFull Paper
2019Cunin, P;Bouslama, R;Machlus, KR;Martínez-Bonet, M;Lee, PY;Wactor, A;Nelson-Maney, N;Morris, A;Guo, L;Weyrich, A;Sola-Visner, M;Boilard, E;Italiano, JE;Nigrovic, PA;Megakaryocyte emperipolesis mediates membrane transfer from intracytoplasmic neutrophils to plateletseLife31042146and Schulze, 2005). Fetal liver-derived MKs were generated as described (Machlus et al., 2017). Human MK generation: mobilized peripheral blood or BM CD34 +stem/progenitor cells were purchased from AllCells. 1 × 105 cells were cultured in StemSpan meFull Paper
2019Dura-Bernal, S;Suter, BA;Gleeson, P;Cantarelli, M;Quintana, A;Rodriguez, F;Kedziora, DJ;Chadderdon, GL;Kerr, CC;Neymotin, SA;McDougal, RA;Hines, M;Shepherd, GM;Lytton, WW;NetPyNE, a tool for data-driven multiscale modeling of brain circuitseLife31025934ode to disk file and combines these files after the simulation has ended. After gathering, the built-in analysis functions have direct access to all the network and simulation output data via sim.net.allCells and sim.allSimData. Importing and exportFull Paper
2019Frank, ND;Jones, ME;Vang, B;Coeshott, C;Evaluation of reagents used to coat the hollow-fiber bioreactor membrane of the Quantum® Cell Expansion System for the culture of human mesenchymal stem cellsMaterials science & engineering. C, Materials for biological applications30606590Healthy, fresh, adult human whole bone marrow (WBM) was purchased either from AllCells LLC (Alameda, CA) or from Lonza (Basel, Switzerland). Flow cytometry staining buffer was SaveFull Paper
2019Laner-Plamberger, S;Oeller, M;Mrazek, C;Hartl, A;Sonderegger, A;Rohde, E;Strunk, D;Schallmoser, K;Upregulation of mitotic bookmarking factors during enhanced proliferation of human stromal cells in human platelet lysateJournal of translational medicine31888679ed from white adipose tissue (WAT) and umbilical cord (UC) after signed informed consent by the donors as described previously [26, 30, 31]. Bone marrow (BM)-derived stromal cells were purchased from AllCells, (Alameda, CA, USA, http://www.allcells.cFull Paper
2019Antebi, B;Asher, AM;Rodriguez, LA;Moore, RK;Mohammadipoor, A;Cancio, LC;Cryopreserved mesenchymal stem cells regain functional potency following a 24-h acclimation periodJournal of translational medicine31464641Human MSCs were isolated from fresh bone-marrow mononuclear cells purchased from AllCells (Alameda, CA). The MSCs were expanded in complete culture media (CCM) consisting SaveFull Paper
2019Coeshott, C;Vang, B;Jones, M;Nankervis, B;Large-scale expansion and characterization of CD3+ T-cells in the Quantum® Cell Expansion SystemJournal of translational medicine31391068g and unique dual-loop circuits. Materials and methods Preparation of peripheral blood mononuclear cells (PBMCs) Peripheral blood leukapheresis products were obtained from individual healthy donors (AllCells, LLC, Alameda, CA, HemaCare Corp, Van NuyFull Paper
2019Shi, L;Zhong, Z;Li, X;Zhou, Y;Pan, Z;Discovery of an Orally Available Janus Kinase 3 Selective Covalent InhibitorJournal of medicinal chemistry30615446Frozen PBMCs (from Allcells) were thawed in RPMI1640 (Thermo Fisher) containing 10% FBS and recovered overnight at 37 °C. The next day, cells were diluted to 1 × 106 cells/mL and seeded (500 μL) in 6-well plates. Compounds or DMSO (5 μL, seriallyFull Paper
2019Zoine, JT;Knight, KA;Fleischer, LC;Sutton, KS;Goldsmith, KC;Doering, CB;Spencer, HT;Ex vivo expanded patient-derived γδ T-cell immunotherapy enhances neuroblastoma tumor regression in a murine modelOncoimmunology31413905Commercially available healthy donor frozen PBMCs were obtained from AllCells (Alameda, CA). At the time of stem cell collection, each patient had undergone two cycles of induction SaveFull Paper
2019Rodriguez, LA;Mohammadipoor, A;Alvarado, L;Kamucheka, RM;Asher, AM;Cancio, LC;Antebi, B;Preconditioning in an Inflammatory Milieu Augments the Immunotherapeutic Function of Mesenchymal Stromal CellsCells31096722D) for immune- and/or inflammatory-mediated diseases. 2. Materials and Methods 2.1. Isolation of BM-MSCs and AD-MSCs Human BM-MSCs were isolated from commercially available mononuclear cells (MNCs) (AllCells LLC; Emeryville, CA, USA), as previouslyFull Paper
2019Youn, M;Huang, H;Chen, C;Kam, S;Wilkes, MC;Chae, HD;Sridhar, KJ;Greenberg, PL;Glader, B;Narla, A;Lin, S;Sakamoto, KM;MMP9 inhibition increases erythropoiesis in RPS14-deficient del(5q) MDS models through suppression of TGF-β pathwaysBlood advances31540902Primary human CD34 + hematopoietic stem/progenitor cells were purified from bone marrow aspirates purchased from Allcells and Stemcell Technologies. CD34 + cells were purified SaveFull Paper
2019Li, C;Mathews, V;Kim, S;George, B;Hebert, K;Jiang, H;Li, C;Zhu, Y;Keesler, DA;Boelens, JJ;Dvorak, CC;Agarwal, R;Auletta, JJ;Goyal, RK;Hanna, R;Kasow, K;Shenoy, S;Smith, AR;Walters, MC;Eapen, M;Related and unrelated donor transplantation for β-thalassemia major: results of an international surveyBlood advances31471325Full Paper
2019Yang, HM;Choi, JJ;Kim, HN;Yang, SJ;Park, SJ;Kang, C;Chung, HM;Lee, MR;Kim, SJ;Moon, SH;Reconstituting Human Cutaneous Regeneration in Humanized Mice under Endothelial Cell TherapyThe Journal of investigative dermatology30393080The derivation of CB-EPCs was performed from CB mononuclear cells, which were purchased from AllCells (Alameda, CA). Briefly, CB mononuclear cells were seeded at a density of 4-6 × 106/mL in a collagen-coated tissue culture dish and cultured in EGM-2Full Paper
2019Jeng, MY;Mumbach, MR;Granja, JM;Satpathy, AT;Chang, HY;Chang, ALS;Enhancer Connectome Nominates Target Genes of Inherited Risk Variants from Inflammatory Skin DisordersThe Journal of investigative dermatology30315781Briefly, donor human peripheral blood cells were obtained from AllCells (Emeryville, CA) and were sorted based on the following markers: CD4 + CD25 − CD45RA + (naive), CD4 + SaveFull Paper
2019Naipauer, J;Rosario, S;Gupta, S;Premer, C;Méndez-Solís, O;Schlesinger, M;Ponzinibbio, V;Jain, V;Gay, L;Renne, R;Chan, HL;Morey, L;Salyakina, D;Abba, M;Williams, S;Hare, JM;Goldschmidt-Clermont, PJ;Mesri, EA;PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environmentPLoS pathogens31881074n SCA-1; and (iii) the ability to differentiate into adipocytes or osteoblast-like cells. Human MSCs were isolated as described previously [39]. Briefly, BM aspirates (25-50 mL) were purchased from AllCells (Emeryville, CA) under appropriate informedFull Paper
2019Bi, W;Xu, W;Cheng, L;Xue, J;Wang, Q;Yu, F;Xia, S;Wang, Q;Li, G;Qin, C;Lu, L;Su, L;Jiang, S;IgG Fc-binding motif-conjugated HIV-1 fusion inhibitor exhibits improved potency and in vivo half-life: Potential application in combination with broad neutralizing antibodiesPLoS pathogens31805154Env (2.5 × 104 cells) as the target cells were seeded into a 96-well plate (25 μL/well), and the mixture of a serially diluted inhibitors and 10E8 (1.5 μg/mL) was added to the culture plate. PBMC (AllCells) as effector cells (2.5 × 104 cells in 25 μLFull Paper
2019Wang, W;Liu, T;Yang, L;Ma, Y;Dou, F;Shi, L;Wen, A;Ding, Y;Study on the multi-targets mechanism of triphala on cardio-cerebral vascular diseases based on network pharmacologyBiomedicine & pharmacotherapy = Biomedecine & pharmacotherapie31112872Triphala was obtained from Tibet Jinzhu Along Tibetan Medicine Co. Ltd. (Tibet, China). The Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from AllCells (Emeryville, CA, USA). Primary antibodies against β-actin, PTGS2, MMP9 and IL6 weFull Paper
2019Acosta, SA;Lee, JY;Nguyen, H;Kaneko, Y;Borlongan, CV;Endothelial Progenitor Cells Modulate Inflammation-Associated Stroke VasculomeStem cell reviews and reports30739275linded to the treatment condition. Cell Culture Procedures EPC Differentiation from Human Bone Marrow Mesenchymal Stem Cells Frozen human bone marrow CD34+ mesenchymal stem cells were purchased from ALLCELLS (Catalog# ABM017F; Alameda, CA). After thFull Paper
2019Meehan, S;Kolyagin, GA;Parks, D;Youngyunpipatkul, J;Herzenberg, LA;Walther, G;Ghosn, EEB;Orlova, DY;Automated subset identification and characterization pipeline for multidimensional flow and mass cytometry data clustering and visualizationCommunications biology31240267Fresh bone marrow mononuclear cells from adult healthy donors were obtained commercially from AllCells, LLC (Quincy, MA, Cat # ABM024). Data was collected for about 0.5-1.0 × 10 SaveFull Paper
2019Guo, X;Yuan, Z;Xu, Y;Zhao, X;Fang, Z;Yuan, WE;A Low-Molecular-Weight Polyethylenimine/pDNA-VEGF Polyplex System Constructed in a One-Pot Manner for Hindlimb Ischemia TherapyPharmaceutics30965617Aldrich. VEGF-A (1239 bp) pDNA encoding GFP was constructed by Bioroot Biology (Shanghai, China). 2.2. Cell Culture Human umbilical vein endothelial cells (HUVECs, primary cells) were purchased from ALLCELLS (Shanghai, China) and cultured in RPMI-16Full Paper
2019Zhang, M;Xu, Y;Jiang, L;Irisin attenuates oxidized low-density lipoprotein impaired angiogenesis through AKT/mTOR/S6K1/Nrf2 pathwayJournal of cellular physiology30942905Commercial HUVECs were obtained from Allcells (Shanghai, China). Cells were seeded in DMEM/F12 medium with 20% FBS (Gibco), 60 μg/ml endothelial cell growth supplement (BD, San Diego, CA) and maintained at 37°C in a CO2 incubator. The HMEC-1 cells weFull Paper
2019Wang, J;Fei, K;Jing, H;Wu, Z;Wu, W;Zhou, S;Ni, H;Chen, B;Xiong, Y;Liu, Y;Peng, B;Yu, D;Jiang, H;Liu, J;Durable blockade of PD-1 signaling links preclinical efficacy of sintilimab to its clinical benefitmAbs31402780× MSD read buffer with surfactant (R92TC-1, MSD) was added into each well. The electrochemiluminescent signal was then measured on the MSD SQ120 instrument. In vitro PD-1 receptor occupancy PBMCs (AllCells, Alameda, California, USA) were activated bFull Paper
2019Ovacik, AM;Li, J;Lemper, M;Danilenko, D;Stagg, N;Mathieu, M;Ellerman, D;Gupta, V;Kalia, N;Nguy, T;Plaks, V;David Johnson, C;Wang, W;Brumm, J;Fine, B;Junttila, T;Lin, K;Carter, PJ;Prabhu, S;Spiess, C;Kamath, AV;Single cell-produced and in vitro-assembled anti-FcRH5/CD3 T-cell dependent bispecific antibodies have similar in vitro and in vivo propertiesmAbs30550367100 (number of live target cells without TDB - number of live target cells with TDB)/number of live target cells without TDB. Human BM aspirates from healthy donors (n = 2, male) (procured from AllCells, ABM001-1) were diluted in phosphate-bufferedFull Paper
2019Wang, H;He, H;Yang, C;miR-342 suppresses the proliferation and invasion of acute myeloid leukemia by targeting Naa10pArtificial cells, nanomedicine, and biotechnology31496296The MNC normal control samples were isolated from normal BM cells purchased from AllCells, LLC (Emeryville, CA) by use of NycoPrep 1.077 A (Axis-Shield, Oslo, Norway) according SaveFull Paper
2019Chang, AY;Hsu, E;Patel, J;Li, Y;Zhang, M;Iguchi, H;Rogoff, HA;Evaluation of Tumor Cell-Tumor Microenvironment Component Interactions as Potential Predictors of Patient Response to NapabucasinMolecular cancer research : MCR31043490pSTAT3 is a potential biomarker for patient response to the anticancer drug napabucasin. Visual Overview: http://mcr.aacrjournals.org/content/molcanres/17/7/1429/F1.large.jpg . SaveFull Paper
2019Marklein, RA;Klinker, MW;Drake, KA;Polikowsky, HG;Lessey-Morillon, EC;Bauer, SR;Morphological profiling using machine learning reveals emergent subpopulations of interferon-γ-stimulated mesenchymal stromal cells that predict immunosuppressionCytotherapy30503100Human bone marrow-derived MSCs were obtained from 11 different donors purchased from Lonza and AllCells at passage two (see Supplementary Table 1 for donor specifications). A SaveFull Paper
2019Sei, J;Moses, B;Becker, A;Kim, M;Kaur, N;Vemuri, M;Civin, C;StemPro HSC expansion medium (Prototype) supports superior expansion of human hematopoietic stem-progenitor cellsCytotherapySingle donor, cord-blood derived, CD34+ cells obtained from AllCells were thawed and cultured in CTS StemPro HSC expansion media containing SCF, FLT3, TPO, IL3 and IL6 growth SaveFull Paper
2019Kou, X;Sun, Y;Li, S;Bian, W;Liu, Z;Zhang, D;Jiang, J;Pharmacology Study of the Multiple Angiogenesis Inhibitor RC28-E on Anti-Fibrosis in a Chemically Induced Lung Injury ModelBiomolecules31652997The human skin fibroblast cell line Detroit 551 was purchased from AllCells (Shanghai, China) and maintained in MEM medium (Gibco, Grand Island, NY, USA) supplemented with 10% SaveFull Paper
2019Zhang, Y;Yu, S;Tuazon, JP;Lee, JY;Corey, S;Kvederis, L;Kingsbury, C;Kaneko, Y;Borlongan, CV;Neuroprotective effects of human bone marrow mesenchymal stem cells against cerebral ischemia are mediated in part by an anti-apoptotic mechanismNeural regeneration research30632499Bcl-2 antibody was 58, 117, or 235 ng/mL. Both hMSC and Bcl-2 doses were based on pilot studies demonstrating their potencies. Cryopreserved human bone marrow CD34+ cells (hBM34+) were purchased from AllCells (Alameda, CA, USA). The exposure time ofFull Paper
2019Weisgrau, KL;Vosler, LJ;Pomplun, NL;Hayes, JM;Simmons, HA;Friedrichs, KR;Rakasz, EG;Neutrophil progenitor populations of rhesus macaquesJournal of leukocyte biology30395351We purchased de-identified, freshly obtained human bone marrow and matching blood samples from 18-65 years old healthy individuals from AllCells LLC (Boston, USA), or Lonza Inc. (Mapleton, IL, USA). The donors signed a procedure-specific consent formFull Paper
2019Cao, L;Weetall, M;Trotta, C;Cintron, K;Ma, J;Kim, MJ;Furia, B;Romfo, C;Graci, JD;Li, W;Du, J;Sheedy, J;Hedrick, J;Risher, N;Yeh, S;Qi, H;Arasu, T;Hwang, S;Lennox, W;Kong, R;Petruska, J;Moon, YC;Babiak, J;Davis, TW;Jacobson, A;Almstead, NG;Branstrom, A;Colacino, JM;Peltz, SW;Targeting of Hematologic Malignancies with PTC299, A Novel Potent Inhibitor of Dihydroorotate Dehydrogenase with Favorable Pharmaceutical PropertiesMolecular cancer therapeutics30352802AML cells, purchased from AllCells (January 2017), Inc and Champions Oncology, Inc (April 2017), were cultured in StemSpan medium (Stemcell Technologies, Vancouver). Cell lines in culture were routinely verified once per month to be mycoplasma free aFull Paper
2019Boothello, RS;Patel, NJ;Sharon, C;Abdelfadiel, EI;Morla, S;Brophy, DF;Lippman, HR;Desai, UR;Patel, BB;A Unique Nonsaccharide Mimetic of Heparin Hexasaccharide Inhibits Colon Cancer Stem Cells via p38 MAP Kinase ActivationMolecular cancer therapeutics30337351The cells were resuspended in 10 mL of Iscove's modified Dulbecco's medium (2% FBS media; Allcells) and mixed with Methyl cellulose (R&D systems), and 1.1 mL of the final cell SaveFull Paper
2019Suhito, IR;Kang, ES;Kim, DS;Baek, S;Park, SJ;Moon, SH;Luo, Z;Lee, D;Min, J;Kim, TH;High density gold nanostructure composites for precise electrochemical detection of human embryonic stem cells in cell mixtureColloids and surfaces. B, Biointerfaces31082776For the comparison, cryopreserved hCB-EPCs were purchased from AllCells, LLC (California, USA) [26,27]. The hCB-EPCs were thawed in a 37 °C warm bath for 2 min and washed twice with 100 μl DNase (Sigma-Aldrich) in 10 ml PBS. Cells were cultured on aFull Paper
2019Xu, P;Ganaie, SS;Wang, X;Wang, Z;Kleiboeker, S;Horton, NC;Heier, RF;Meyers, MJ;Tavis, JE;Qiu, J;Endonuclease Activity Inhibition of the NS1 Protein of Parvovirus B19 as a Novel Target for Antiviral Drug DevelopmentAntimicrobial agents and chemotherapy30530599Primary human CD34+ hematopoietic stem cells (HSCs) were isolated from the bone marrow of a healthy human donor. We purchased them from AllCells LLC (Alameda, CA), and B19V-containng plasma samples were obtained from ViraCor Eurofins Laboratories (LeFull Paper
2019Patel, PR;Senyuk, V;Rodriguez, NS;Oh, AL;Bonetti, E;Mahmud, D;Barosi, G;Mahmud, N;Rondelli, D;Synergistic Cytotoxic Effect of Busulfan and the PARP Inhibitor Veliparib in Myeloproliferative NeoplasmsBiology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation30615982Normal CD34 + cells used as healthy controls were obtained from apheresis collection or cord blood (AllCells, Alameda, CA). Mononuclear cells were subsequently obtained by SaveFull Paper
2019Campos, JM;Sousa, AC;Caseiro, AR;Pedrosa, SS;Pinto, PO;Branquinho, MV;Amorim, I;Santos, JD;Pereira, T;Mendonça, CM;Afonso, A;Atayde, LM;Maurício, AC;Dental pulp stem cells and Bonelike® for bone regeneration in ovine modelRegenerative biomaterials30740242onmental SEM with X-ray microanalysis and electron backscattered diffraction analysis: Quanta 400 FEG ESEM/EDAX Genesis X4M . hMSC preparation Cell culture and maintenance hDPSCs were obtained from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01)Full Paper
2019Dai, L;Hu, W;Yang, Z;Chen, D;He, B;Chen, Y;Zhou, L;Xie, H;Wu, J;Zheng, S;Upregulated expression of HOXB7 in intrahepatic cholangiocarcinoma is associated with tumor cell metastasis and poor prognosisLaboratory investigation; a journal of technical methods and pathology30664713Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Shanghai, China; allcells.biomart.cn) and maintained in DMEM supplemented with 10% fetal bovine SaveFull Paper
2019Sachdeva, M;Duchateau, P;Depil, S;Poirot, L;Valton, J;Granulocyte-macrophage colony-stimulating factor inactivation in CAR T-cells prevents monocyte-dependent release of key cytokine release syndrome mediatorsThe Journal of biological chemistry30804212Cryopreserved human PBMCs were acquired from ALLCELLS (catalog no. PB006F), and human monocytes were acquired from STEMCELL Technologies (catalog no. 70035.1). Both PBMCs and monocytes were cultured in X-vivo-15 media (Lonza, catalog no. BE04-418Q),Full Paper
2019Yamamoto, R;Xu, Y;Ikeda, S;Sumida, K;Tanaka, H;Hozumi, K;Takaori-Kondo, A;Minato, N;Thymic Development of a Unique Bone Marrow-Resident Innate-like T Cell Subset with a Potent Innate Immune FunctionJournal of immunology (Baltimore, Md. : 1950)31085589Human BM mononuclear cells from healthy donors were purchased from AllCells (Alameda, CA). T cells, B cells, myeloid cells, and dendritic cells were isolated from BM cells by SaveFull Paper
2019Nikitin, PA;Rose, EL;Byun, TS;Parry, GC;Panicker, S;C1s Inhibition by BIVV009 (Sutimlimab) Prevents Complement-Enhanced Activation of Autoimmune Human B Cells In VitroJournal of immunology (Baltimore, Md. : 1950)30635392Primary PBMC from de-identified patients with rheumatoid arthritis were purchased from AllCells. No therapeutic modalities were used as exclusion criteria for samples from patients SaveFull Paper
2019Wu, J;Chen, L;Wang, R;Song, Z;Shen, Z;Zhao, Y;Huang, S;Lin, Z;Exosomes Secreted by Stem Cells from Human Exfoliated Deciduous Teeth Promote Alveolar Bone Defect Repair through the Regulation of Angiogenesis and OsteogenesisACS biomaterials science & engineering33405738(FBS; Gibco), 100 U/mL penicillin G, as well as 100 mg/mL streptomycin. HUVECs were bought from ALLCELLS (Shanghai, China) and expanded in endothelial cell growth medium (EGM). BMSCs were harvested from the femoral bones of SD rats. All experimentalFull Paper
2019Huang, YJ;Lee, CY;Cao, J;Lee, HS;Chang, CH;Chen, PD;Wu, YM;Therapeutic Potential of Plasma Proteins Derived from Umbilical Cord Blood for Acute Liver FailureMolecular pharmaceutics30698974There are very limited clinically viable treatment options for acute liver failure, a life-threatening condition that rapidly progresses to loss of liver function. In this study, we aim to evaluate SaveFull Paper
2019Seo, A;Gulsuner, S;Pierce, S;Ben-Harosh, M;Shalev, H;Walsh, T;Krasnov, T;Dgany, O;Doulatov, S;Tamary, H;Shimamura, A;King, MC;Inherited thrombocytopenia associated with mutation of UDP-galactose-4-epimerase (GALE)Human molecular genetics30247636CD34+ cells from human cord blood were purchased from AllCells. Thawed cells were expanded for 4 h at 37C in tissue culture incubator with X-VIVO10 media (Lonza) supplemented SaveFull Paper
2019Moore, N;Chevillet, JR;Healey, LJ;McBrine, C;Doty, D;Santos, J;Teece, B;Truslow, J;Mott, V;Hsi, P;Tandon, V;Borenstein, JT;Balestrini, J;Kotz, K;A Microfluidic Device to Enhance Viral Transduction Efficiency During Manufacture of Engineered Cellular TherapiesScientific reports31641163Both Research Blood Components and AllCells obtain IRB approved consent from all donors giving permission to collect their blood or blood product and use or sell it for research SaveFull Paper
2019Fischer, CL;Bates, AM;Lanzel, EA;Guthmiller, JM;Johnson, GK;Singh, NK;Kumar, A;Vidva, R;Abbasi, T;Vali, S;Xie, XJ;Zeng, E;Brogden, KA;Computational Models Accurately Predict Multi-Cell Biomarker Profiles in Inflammation and CancerScientific reports31350446experiments, GE KER were suspended at 1 × 105 viable cells/ml in Lymphocyte Growth Medium-3 (LGM-3, Lonza Walkersville, Inc., Walkersville, MD). Human monocyte-derived immature myeloid DC (DC, AllCells, Alameda, CA) were positively identified by floFull Paper
2019Laner-Plamberger, S;Oeller, M;Poupardin, R;Krisch, L;Hochmann, S;Kalathur, R;Pachler, K;Kreutzer, C;Erdmann, G;Rohde, E;Strunk, D;Schallmoser, K;Heparin Differentially Impacts Gene Expression of Stromal Cells from Various TissuesScientific reports31076619ed by the ethical committee of federal state of Salzburg. All donors of BM, WAT and UC signed an informed consent concerning the research use of materials. Additionally, BM samples were obtained from AllCells, (Alameda, CA, USA, www.allcells.com/statFull Paper
2019Yea, K;Jones, TM;Jung, D;Shin, S;Arlian, BM;Han, KH;Zha, Z;Kim, MS;Oh, YS;Zhang, H;Lerner, RA;An agonist antibody prefers relapsed AML for induction of cells that kill each otherScientific reports30837591population studied here is much larger than that simply defined by CD34+ and CD38+ cells. Methods Culture of AML cells Human AML cells were isolated from patients and frozen at −80 °C (ABM010, AllCells). The informed consent was obtained from all paFull Paper
2019Das, P;Panda, SK;Agarwal, B;Behera, S;Ali, SM;Pulse, ME;Solomkin, JS;Opal, SM;Bhandari, V;Acharya, S;Novel Chitohexaose Analog Protects Young and Aged mice from CLP Induced Polymicrobial SepsisScientific reports30814582mg/kg to young adult mice and 25 mg/kg to aged mice in a 0.2 mL volume near the dorsal scapular region. Cell lines Human peripheral blood mononuclear cells (HPBMCs) were purchased from LeukoPaK (AllCells); 0.5 × 106 cells/well were seeded in 96-wellFull Paper
2019Kizilyer, A;Singh, MV;Singh, VB;Suwunnakorn, S;Palis, J;Maggirwar, SB;Inhibition of Tropomyosin Receptor Kinase A Signaling Negatively Regulates Megakaryopoiesis and induces ThrombopoiesisScientific reports30808933CD34+ cells derived from BM or umbilical cord blood were purchased from Allcells (Emeryville, CA, USA), and differentiated as previously described 48 . For inducing MK progenitor ( SaveFull Paper
2019Zarriello, S;Neal, EG;Kaneko, Y;Borlongan, CV;T-Regulatory Cells Confer Increased Myelination and Stem Cell Activity after Stroke-Induced White Matter InjuryJournal of clinical medicine31010132Purified BMSCs were obtained and stored in aliquots in dry ice (AllCells, Almeda, CA, USA). After the cells reached confluence, they were subjected to an oxygen-glucose deprivation SaveFull Paper
2019Hu, B;Zou, Y;Zhang, L;Tang, J;Niedermann, G;Firat, E;Huang, X;Zhu, X;Nucleofection with Plasmid DNA for CRISPR/Cas9-Mediated Inactivation of Programmed Cell Death Protein 1 in CD133-Specific CAR T CellsHuman gene therapy29706119Frozen peripheral blood mononuclear cells (PBMCs) from healthy donors were purchased from ALLCELLS (PB005F). The tumor cell lines used in this study were generated based on a SaveFull Paper
2019Bednar, KJ;Nycholat, CM;Rao, TS;Paulson, JC;Fung-Leung, WP;Macauley, MS;Exploiting CD22 To Selectively Tolerize Autoantibody Producing B-Cells in Rheumatoid ArthritisACS chemical biology30835424Frozen peripheral blood mononuclear cells (PBMCs) were obtained through AllCells (Alameda, CA) or BioreclamationIVT (Chestertown, MD) as per institutional protocols for human sample acquisition. Cells were thawed in accordance with the manufacture’sFull Paper
2019Schott, JW;León-Rico, D;Ferreira, CB;Buckland, KF;Santilli, G;Armant, MA;Schambach, A;Cavazza, A;Thrasher, AJ;Enhancing Lentiviral and Alpharetroviral Transduction of Human Hematopoietic Stem Cells for Clinical ApplicationMolecular therapy. Methods & clinical development31338385lus HSC Brew GMP supplement (Miltenyi Biotec, Surrey, UK) additionally supplemented with 2% human albumin. For the GMP experiment, HD CD34+ HSPCs were isolated from a fresh mobilized leukapheresis (AllCells, Alameda, CA, USA) following standard immuFull Paper
2019Ellison, SM;Liao, A;Wood, S;Taylor, J;Youshani, AS;Rowlston, S;Parker, H;Armant, M;Biffi, A;Chan, L;Farzaneh, F;Wynn, R;Jones, SA;Heal, P;Gaspar, HB;Bigger, BW;Pre-clinical Safety and Efficacy of Lentiviral Vector-Mediated Ex Vivo Stem Cell Gene Therapy for the Treatment of Mucopolysaccharidosis IIIAMolecular therapy. Methods & clinical development31044143CD34 + cells were positively selected from two pooled PB mononuclear cell (PBMC) apheresis units (Allcells, USA) using the Miltenyi CliniMACSPlus instrument and reagents Save;00 for a further 24 hrs prior to seeding in CFU assay. Large-Scale TransFull Paper
2019Liu, W;Zhang, C;Wang, L;Huang, X;Zhang, J;He, Y;Chen, L;Li, J;Successful reversal of ovarian hyperstimulation syndrome in a mouse model by rapamycin, an mTOR pathway inhibitorMolecular human reproduction31329230HUVEC were purchased from ALLCELLS and seeded on micropore inserts (pore size 0.4 μm, Corning) in a density of 3 × 10 5 cells per insert and cultured overnight with or without 40 SaveFull Paper
2019Cheng, Y;Dai, X;Yang, T;Zhang, N;Liu, Z;Jiang, Y;Low Long Noncoding RNA Growth Arrest-Specific Transcript 5 Expression in the Exosomes of Lung Cancer Cells Promotes Tumor AngiogenesisJournal of oncology31186629ells were purchased from the Chinese Academy of Sciences Type Culture Collections Cell Bank (Shanghai, China). The primary human umbilical vein endothelial cells (HUVECs) were purchased from Shanghai AllCells Biotechnology Company (Shanghai, China).Full Paper
2019Kim, CH;Lim, CY;Lee, JH;Kim, KC;Ahn, JY;Lee, EJ;Human Embryonic Stem Cells-Derived Mesenchymal Stem Cells Reduce the Symptom of Psoriasis in Imiquimod-Induced Skin ModelTissue engineering and regenerative medicine30815354The PBMCs were purchased from AllCells, LLC. The hE-MSCs were inactivated by treatment with 10 μg/mL mitomycin-C (Sigma-Aldrich, St. Louis, MO, USA) for 1 h at 37 C. SaveFull Paper
2019Geldenhuys, WJ;Nair, RR;Piktel, D;Martin, KH;Gibson, LF;The MitoNEET Ligand NL-1 Mediates Antileukemic Activity in Drug-Resistant B-Cell Acute Lymphoblastic LeukemiaThe Journal of pharmacology and experimental therapeutics31010844Primary CD3 + T cells, CD19 + B cells, peripheral blood mononuclear cells, and bone marrow mononuclear cells were purchased from AllCells (Allcells.com) and maintained in SaveFull Paper
2019Xu, L;Zhao, G;Zhu, H;Wang, S;Sun, A;Zou, Y;Ge, J;Peroxisome Proliferator-Activated Receptor-γ Antagonizes LOX-1-Mediated Endothelial Injury by Transcriptional Activation of miR-590-5pPPAR research31354796Quantitative analysis of stained lipid area was performed by a blinded observer using Leica QWin V3 software. 2.3. In Vitro HUVECs Culture and Cell Immunostaining Primary HUVECs were purchased from AllCells (Shanghai, China) and cultured in low-glucFull Paper
2019Zhao, S;Molina, A;Yu, A;Hanson, J;Cheung, H;Li, X;Natkunam, Y;High frequency of CD74 expression in lymphomas: implications for targeted therapy using a novel anti-CD74-drug conjugateThe journal of pathology. Clinical research30191677egression analysis with one site‐specific binding equation on GraphPad Prism (La Jolla, CA, USA). Whole bone marrow (BM) aspirates (3 ml each) from five healthy human donors was obtained through AllCells, Inc. (Alameda, CA, USA). FACS binding on BMFull Paper
2019Xia, F;Yang, C;Wang, Y;Youcef-Toumi, K;Reuter, C;Ivanov, T;Holz, M;Rangelow, IW;Lights Out! Nano-Scale Topography Imaging of Sample Surface in Opaque Liquid Environments with Coated Active Cantilever ProbesNanomaterials (Basel, Switzerland)31337145Immersion test for whole blood sample with Ethylenediaminetetraacetic acid (EDTA) anti-coagulation treatment from AllCells LLC was also conducted. The coated probe worked well at SaveFull Paper
2019Yu, Y;Yoo, SM;Park, HH;Baek, SY;Kim, YJ;Lee, S;Kim, YL;Seo, KW;Kang, KS;Preconditioning with interleukin-1 beta and interferon-gamma enhances the efficacy of human umbilical cord blood-derived mesenchymal stem cells-based therapy via enhancing prostaglandin E2 secretion and indoleamine 2,3-dioxygenase activity in dextran sulfate sodium-induced colitisJournal of tissue engineering and regenerative medicine312930882.7 Isolation of CD4+ T cells with magnetic-activated cell sorting (MACS) CD4+ T cells were purified from PBMCs (ALLCells, Alameda, CA, USA) using MACS (Miltenyi Biotec, Bergisch Gladbach, Germany) as previously discribed (Hong et al., 2017)Full Paper
2019Yin, Y;Guo, J;Teng, F;Yu, L;Jiang, Y;Xie, K;Jiang, M;Fang, J;Preparation of a Novel One-Armed Anti-c-Met Antibody with Antitumor Activity Against Hepatocellular CarcinomaDrug design, development and therapy31849449bovine serum (FBS, qualified, Australia#10099-141; Gibco Co., Ltd) in a humidified incubator in the presence of 5% CO2 at 37°C. Human umbilical vein endothelial cells (HUVECs) were obtained from the Allcells Co., Ltd. (Shanghai, China). The cells werFull Paper
2019Kono, M;Matsuhiroya, S;Nakazawa, F;Kaido, M;Wada, A;Tomiyama, Y;Morphological and optical properties of human immature platelet-enriched population produced in immunodeficient micePlatelets30059642The animals were fed a pellet chow and sterilized tap water ad libitum. Human CD34-positive cells (1 × 105) from cord blood (AllCells, CA, USA) were transplanted through the tail vein after irradiation with 2.5 Gy. Eltrombopag at 25 mg/kg body weightFull Paper
2019Gangolli, RA;Devlin, SM;Gerstenhaber, JA;Lelkes, PI;Yang, M;A Bilayered Poly (Lactic-Co-Glycolic Acid) Scaffold Provides Differential Cues for the Differentiation of Dental Pulp Stem CellsTissue engineering. Part A29984629Human DPSCs were purchased from AllCells LLC (Emeryville, CA), and cultured in a basal medium comprised of 85% MEM Alpha (Gibco ThermoFisher Scientific, Waltham, MA) supplemented with 15% fetal bovine serum (Stem Cell qualified; ThermoFisher ScientifFull Paper
2019Huang, L;Tan, J;Li, W;Zhou, L;Liu, Z;Luo, B;Lu, L;Zhou, C;Functional polyhedral oligomeric silsesquioxane reinforced poly(lactic acid) nanocomposites for biomedical applicationsJournal of the mechanical behavior of biomedical materials30500698The sterilization step of samples was similar to above films. HUVECs (purchased from Allcells, shanghai, China) were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS and 1% penicillin/streptomycin (P/S, Gibco)Full Paper
2019Cackowski, FC;Wang, Y;Decker, JT;Sifuentes, C;Weindorf, S;Jung, Y;Wang, Y;Decker, AM;Yumoto, K;Szerlip, N;Buttitta, L;Pienta, KJ;Morgan, TM;Taichman, RS;Detection and isolation of disseminated tumor cells in bone marrow of patients with clinically localized prostate cancerThe Prostate31449673Normal marrow samples were purchased from AllCells (Alameda, CA), drawn from the posterior superior iliac crest of paid donors, transferred to heparinized tubes and shipped SaveFull Paper
2019Zheng, Q;Zhang, X;Yang, H;Xie, J;Xie, Y;Chen, J;Yu, C;Zhong, C;Internal Ribosome Entry Site Dramatically Reduces Transgene Expression in Hematopoietic Cells in a Position-Dependent MannerViruses31597367anassas, VA, USA). Human embryonic kidney cell line 293T was purchased from the Institute of Biochemistry Cell Biology (Shanghai, China). The CD34+ hematopoietic stem cells (HSCs) were purchased from ALLCELLS (Alameda, CA, USA). The hepatocellular caFull Paper
2019Zhao, H;Chen, L;Zhong, G;Huang, Y;Zhang, X;Chu, C;Chen, L;Wang, M;Titanium Dioxide Nanoparticles Induce Mitochondrial Dynamic Imbalance and Damage in HT22 CellsJournal of NanomaterialsHT22 cells were from AllCells (Shanghai Biological Technology Co. Ltd., Shanghai, China). We carried out some experiments in the BE(2)C cell line, which is a human neuroblastoma cell line. The cells were planted in Dulbecco’s minimum essential mediumFull Paper
2019Liang, YR;Zhang, T;Jia, P;Xu, XL;Fang, Y;Ding, XQ;Interaction between bone marrow-derived dendritic cells and miR-21 of tubular renal epithelial cells under hypoxiaEuropean review for medical and pharmacological sciences30840288cluster of differentiation 86), MHCII (major histocompatibility complex class II molecules), and CCR7 (eBioscience, Frankfurt, Germany). Primary Murine Renal TEC Culture Primary renal TECs were bought from AllCells (Allcells, LLC, Berkeley, CA, USA)Full Paper
2019Youngblood, BA;Brock, EC;Leung, J;Falahati, R;Bryce, PJ;Bright, J;Williams, J;Shultz, LD;Greiner, DL;Brehm, MA;Bebbington, C;Tomasevic, N;AK002, a Humanized Sialic Acid-Binding Immunoglobulin-Like Lectin-8 Antibody that Induces Antibody-Dependent Cell-Mediated Cytotoxicity against Human Eosinophils and Inhibits Mast Cell-Mediated Anaphylaxis in MiceInternational archives of allergy and immunology31401630Purified human natural killer (NK) cells (AllCells) were washed with culture medium and added at 450,000 cells/well (effector: target cell ratio 9:1). Plates were incubated for 4 h at 37C SaveFull Paper
2019Gautam, A;Donohue, D;Hoke, A;Miller, SA;Srinivasan, S;Sowe, B;Detwiler, L;Lynch, J;Levangie, M;Hammamieh, R;Jett, M;Investigating gene expression profiles of whole blood and peripheral blood mononuclear cells using multiple collection and processing methodsPloS one31809517and the PAXgene blood RNA tubes were immediately frozen, one set of triplicate EDTA tubes was stored at 4C, and the remaining tubes received further processing at the AllCells, SaveFull Paper
2019Caseiro, AR;Santos Pedrosa, S;Ivanova, G;Vieira Branquinho, M;Almeida, A;Faria, F;Amorim, I;Pereira, T;Maurício, AC;Mesenchymal Stem/ Stromal Cells metabolomic and bioactive factors profiles: A comparative analysis on the umbilical cord and dental pulp derived Stem/ Stromal Cells secretomePloS one31774816ngiogenesis. 2. Materials and methods 2.1. Human Mesenchymal Stem/ Stromal Cells characterization and conditioning protocol Umbilical Cord -MSCs [PromoCellTM (C-12971; Lot No. 1112304.2)] and DPSCs [AllCells, LLC (DP0037F, Lot No. DPSC090411-01)] weFull Paper
2019Manshouri, T;Verstovsek, S;Harris, DM;Veletic, I;Zhang, X;Post, SM;Bueso-Ramos, CE;Estrov, Z;Primary myelofibrosis marrow-derived CD14+/CD34- monocytes induce myelofibrosis-like phenotype in immunodeficient mice and give rise to megakaryocytesPloS one31569199nstitutional Review Board (MDACC IRB1) approved written informed consent had been obtained. The patients’ clinical data are provided in S1 Table. As controls, normal BM aspirates were obtained from AllCells (Alameda, CA). Low-density cells were fractFull Paper
2019Singer, JW;Al-Fayoumi, S;Taylor, J;Velichko, S;O'Mahony, A;Comparative phenotypic profiling of the JAK2 inhibitors ruxolitinib, fedratinib, momelotinib, and pacritinib reveals distinct mechanistic signaturesPloS one31560729eline Cell Technologies and Cell Applications. Peripheral blood mononuclear cells (PBMCs) and positively selected primary normal human CD19+ B cells, CD4+ T cells, and CD14+ monocytes were purchased (AllCells, Emeryville, CA) or were isolated in-housFull Paper
2019Gunawardena, TNA;Masoudian, Z;Rahman, MT;Ramasamy, TS;Ramanathan, A;Abu Kasim, NH;Dental derived stem cell conditioned media for hair growth stimulationPloS one31042749ulp stem cells obtained from human deciduous teeth (SHED) in comparison to CM produced using HFSCs. Methodology Cell culturing of SHED and HFSCs SHED and HFSCs Cells at Passage 1 were purchased from AllCells, USA and ICELLTIS, Labege, France respectFull Paper
2019Mamontov, P;Eberwine, RA;Perrigoue, J;Das, A;Friedman, JR;Mora, JR;A negative role for the interleukin-2-inducible T-cell kinase (ITK) in human Foxp3+ TREG differentiationPloS one31022269or pharmacological targeting of specific Tec kinases in inflammatory and autoimmune disease. Materials and methods Cell culture Naïve CD4+ CD45RA+ CD25- peripheral blood T cells were purchased from AllCells (Alameda, CA). For Th0 non-polarized condiFull Paper
2019Rytlewski, J;Deng, S;Xie, T;Davis, C;Robins, H;Yusko, E;Bienkowska, J;Model to improve specificity for identification of clinically-relevant expanded T cells in peripheral bloodPloS one30870493expansion, have broad clinical value. Methods Training cohort Whole blood samples collected from 10 healthy donors at three time points with 2-week intervals between collections were purchased from AllCells, LLC (Emeryville, CA). Among the 10 donorsFull Paper
2019Yoshida, M;Horiguchi, H;Kikuchi, S;Iyama, S;Ikeda, H;Goto, A;Kawano, Y;Murase, K;Takada, K;Miyanishi, K;Kato, J;Kobune, M;miR-7977 inhibits the Hippo-YAP signaling pathway in bone marrow mesenchymal stromal cellsPloS one30835743r127; ab76252) and anti-NF2/Merlin Ab (ab88957) were purchased from Abcam (Tokyo, Japan). Human BM CD34+ cells and three different lot numbers of MSCs from healthy volunteers (HV) were purchased from AllCells, LLC (Toronto, Canada) (HV-derived MSCs #Full Paper
2019Chung, JE;Magis, W;Vu, J;Heo, SJ;Wartiovaara, K;Walters, MC;Kurita, R;Nakamura, Y;Boffelli, D;Martin, DIK;Corn, JE;DeWitt, MA;CRISPR-Cas9 interrogation of a putative fetal globin repressor in human erythroid cellsPloS one30645582based on mobility using a separate FASC hemoglobin standard (Trinity biotech). Electroporation of CD34+ HSPCs with Cas9 RNPs HSPC electroporation was as described [28]. CD34+ HSPCs (G-CSF mobilized, Allcells, Inc.) were thawed and cultured in SFEM (Full Paper
2019Hu, X;Shen, G;Hu, T;Guan, G;Wang, L;Use of an in vitro dynamic culture system to assess flow shear forces upon cell adhesion within different structuresJournal of Chemical Technology & BiotechnologyHuman umbilical vein endothelial cells (HUVEC001), provided by Allcells Company (Shanghai, China), were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, South American) and 1% penicillin-streptomycin (GibFull Paper
2019Satani, N;Giridhar, K;Cai, C;Wewior, N;Norris, DD;Olson, SD;Aronowski, J;Savitz, SI;Aspirin in stroke patients modifies the immunomodulatory interactions of marrow stromal cells and monocytesBrain research31220426MSCs from fresh human bone marrow aspirates (AllCells, Alameda, CA) were prepared from 3 separate healthy volunteers using density centrifugation and plastic adherence as previously described (Diaz et al., 2017, Kota et al., 2017, Parsha et al., 2017Full Paper
2019Mohd Nor, NH;Berahim, Z;Azlina, A;Kannan, TP;Identification of novel fibroblast-like cells from stem cells from human exfoliated deciduous teethClinical oral investigations30847574Objectives This study aimed to differentiate and characterize fibroblast-like cells from stem cells from human exfoliated deciduous teeth (SHED). Materials and methods The SaveFull Paper
2019McIntyre, PW;Wu, JL;Kolte, R;Zhang, R;Gregory, RL;Bruzzaniti, A;Yassen, GH;The antimicrobial properties, cytotoxicity, and differentiation potential of double antibiotic intracanal medicaments loaded into hydrogel systemClinical oral investigations29943369Human dental pulp stem cells (DPSC) (AllCells, Alameda, CA, USA) were cultured in 24-well plates (Cat: TP9024, Alkali Scientific Inc.) at concentrations of 3 × 104 cells/well in α-MEM (HyClone Laboratories Inc., South Logan, UT, USA) with 10% heat-inFull Paper
2019Zhong, X;Gao, W;Wu, R;Liu, H;Ge, J;Dendritic cell exosome‑shuttled miRNA146a regulates exosome‑induced endothelial cell inflammation by inhibiting IRAK‑1: A feedback control mechanismMolecular medicine reports31638185HUVECs were obtained from AllCells, LLC and cultured in ECM (Scien Cell Research Laboratories, Inc.). The 8-10th generations of HUVECs were used in this study and they were harvested for mRNA or protein detection after treatment with exosomes for 6 oFull Paper
2019Chen, T;Li, T;Wang, J;p53 mediates PEDF‑induced autophagy in human umbilical vein endothelial cells through sestrin2 signalingMolecular medicine reports31173218ed to investigate the association between the PEDF-p53-sestrin pathway and autophagy in human umbilical vein endothelial cells (HUVECs). Materials and methods Cell culture HUVECs were purchased from AllCells (Alameda, CA, USA). Cells were cultured iFull Paper
2019Feitosa, SA;Palasuk, J;Geraldeli, S;Windsor, LJ;Bottino, MC;Physicochemical and biological properties of novel chlorhexidine-loaded nanotube-modified dentin adhesiveJournal of biomedical materials research. Part B, Applied biomaterials30199597The cytotoxicity of the CHX-loaded nanotube-modified adhesive and control groups (SBMP and HNT) were evaluated using human dental pulp stem cells (hDPSC, AllCells, Alameda, SaveFull Paper
2019Kim, MM;Schlussel, L;Zhao, L;Himburg, HA;Dickkopf-1 Treatment Stimulates Hematopoietic Regenerative Function in Infused Endothelial Progenitor CellsRadiation research31081743Human bone marrow CD34+ cells (AllCells®, Alameda, CA) were irradiated at 3 Gy and added in noncontact to the upper portion of the transwell at a dose of 10,000 cells/well. Cells were cultured for 7 days, viable cells were enumerated by trypan blue eFull Paper
2019Choi, HW;Chan, C;Shterev, ID;Lynch, HE;Robinette, TJ;Johnson-Weaver, BT;Shi, J;Sempowski, GD;Kim, SY;Dickson, JK;Gooden, DM;Abraham, SN;Staats, HF;Identification of Novel Mast Cell Activators Using Cell-Based High-Throughput ScreeningSLAS discovery : advancing life sciences R & D30917061Primary human mast cells were differentiated from human CD34+ hematopoietic stem cells.19 Human CD34+ hematopoietic stem cells were obtained from commercial sources (Allcells). Isolated CD34+ cells was suspended at 5 × 105 cells/mL in IMDM medium supFull Paper
2019Patel, A;Clementelli, CM;Jarocha, D;Mosoyan, G;Else, C;Kintali, M;Fong, H;Tong, J;Gordon, R;Gillespie, V;Keyzner, A;Poncz, M;Hoffman, R;Iancu-Rubin, C;Pre-clinical development of a cryopreservable megakaryocytic cell product capable of sustained platelet production in miceTransfusion31802511Division of Hematology and Medical Oncology, Tisch Cancer Institute and the Department of Medicine, Icahn School of Medicine at Mount Sinai, New York, New York.++Division of Hematology and Medical Oncology, Tisch Cancer Institute and the Department oFull Paper
2019Md Hashim, S;Yusof, M;Zahari, W;Chandra, H;Ahmad Amin Noordin, K;Kannan, T;Sheikh Abdul Hamid, S;Mokhtar, K;Azlina, A;Human Amniotic Membrane as a Matrix for Endothelial Differentiation of VEGF-Treated Dental Stem CellsCellular and Molecular BioengineeringSHED (AllCells, USA) were cultured in the Minimum Essential Medium (MEM) Alpha Medium (α-MEM) (Gibco, USA) supplemented with 15% of fetal bovine serum (FBS) (Gibco, USA) and 50 U/mL of penicillin-streptomycin (Gibco, USA). The cells were incubated inFull Paper
2019Juillerat, A;Tkach, D;Busser, BW;Temburni, S;Valton, J;Duclert, A;Poirot, L;Depil, S;Duchateau, P;Modulation of chimeric antigen receptor surface expression by a small molecule switchBMC biotechnology31269942witching-off CAR T-cells. Overall, this could mitigate some of the toxicities that could occur with early intense antitumor responses. Methods T-cell proliferation Cryopreserved human PBMCs/T-cells (ALLCELLS, cat # PB006F) were used in accordance wiFull Paper
2019Kim, OH;Yoon, OJ;Lee, HJ;Silk fibroin scaffolds potentiate immunomodulatory function of human mesenchymal stromal cellsBiochemical and biophysical research communications31506179Although mesenchymal stromal cells (MSCs) show great potential for use in regenerative medicine, their therapeutic efficacy remains limited because of their low adaptation efficiency SaveFull Paper
2019Luo, H;Hu, L;Ma, B;Zhao, M;Luo, M;Deng, Q;Deng, S;Ye, H;Lin, T;Chen, J;Wang, T;Zhu, J;Lu, H;Molecular dynamics based improvement of the solubilizing self-cleavable tag Zbasic-ΔI-CM application in the preparation of recombinant proteins in Escherichia coliBiochemical and biophysical research communications30967267HUVEC was obtained from ATCC (Cat# CRL-1730) and cultured in ECM medium supplemented with 5% fetal bovine serum and 1 × ECGS (Allcells; Shanghai, China). For proliferation detection, the cells were harvested and re-suspended using the assay medium (EFull Paper
2019Camarillo, JM;Swaminathan, S;Abshiru, NA;Sikora, JW;Thomas, PM;Kelleher, NL;Coupling Fluorescence-Activated Cell Sorting and Targeted Analysis of Histone Modification Profiles in Primary Human LeukocytesJournal of the American Society for Mass Spectrometry31286445De-identified donor peripheral blood (PB) specimen were obtained from AllCells (Alameda, CA), Innovative Research (Novi, MI), or as clinical discards from the Clinical Flow Cytometry Laboratory at Northwestern Memorial Hospital. All procedures perforFull Paper
2019Ishikawa, G;Fujiwara, N;Hirschfield, H;Varricchio, L;Hoshida, Y;Barosi, G;Rosti, V;Padilla, M;Mazzarini, M;Friedman, SL;Hoffman, R;Migliaccio, AR;Shared and Tissue-Specific Expression Signatures between Bone Marrow from Primary Myelofibrosis and Essential ThrombocythemiaExperimental hematology31678370Bone marrow from eight healthy individuals was purchased from AllCells Technology (Oakland, CA). mRNA in amounts sufficient for analyses was obtained from 6 PMF and all ET SaveFull Paper
2019Will, B;Kao, Y;Chen, J;Aivalioti, M;Tatiparthy, M;1029 - IRON HOMEOSTASIS-REGULATORY PATHWAYS IN HEMATOPOIETIC STEM CELLSExperimental HematologyHealthy cryopreserved human bone marrow cells and CD34+ enriched cells were purchased from AllCells (Alameda, CA) and ReachBio Research Labs (ReachBio LLC, WA). Fresh unprocessed bone marrow cells were purchased from Lonza (Lonza, MD), and the cellsFull Paper
2019Yang, H;Sun, J;Chen, H;Wang, F;Li, Y;Wang, H;Qu, T;Mesenchymal stem cells from bone marrow attenuated the chronic morphine-induced cAMP accumulation in vitroNeuroscience letters30605704Human bone marrow (n = 3) was purchased from AllCells Inc (Emeryville, CA). MSCs were isolated from the bone marrow according to previously described methods [16]. In brief, the bone marrow was diluted 1:1 with DMEM (low glucose, Invitrogen, CA, USA)Full Paper
2019Muruganandham, A;Dumont, C;Xing, K;Approaching stability challenges for flow cytometry in a regulated bioanalytical environmentBioanalysis31637937Mobilized human whole blood, with K 3 EDTA as anticoagulant, was purchased from ALLCELLS . Celerion stabilizer was added at 20% volume of blood within 24 h of collection and SaveFull Paper
2019Choi, SA;An, JH;Lee, SH;Lee, GH;Yang, HJ;Jeong, PS;Cha, JJ;Lee, S;Park, YH;Song, BS;Sim, BW;Kim, YH;Kim, JS;Jin, YB;Huh, JW;Lee, SR;Lee, JH;Kim, SU;Comparative Evaluation of Hormones and Hormone-Like Molecule in Lineage Specification of Human Induced Pluripotent Stem CellsInternational journal of stem cells31242719mising other lineage decisions. Materials and Methods Cell culture, hiPSC generation, and lineage specification To obtain hiPSCs, CD34+ cells were purified from primary human bone marrow (BM) cells (Allcells, Emeryville, CA, USA) via immunomagneticFull Paper
2019Kuramitsu, M;Okuma, K;Tezuka, K;Nakamura, H;Sagara, Y;Kurane, I;Hamaguchi, I;Development and evaluation of human T-cell leukemia virus-1 and -2 multiplex quantitative PCRMicrobiology and immunology31429972Peripheral blood mononuclear cells (PBMCs) were purchased from AllCells Inc. (Alameda, CA), TL-Om1 cells have been reported previously 15 and the MoT cell line (CRL-8066) was purchased from ATCC (Manassas, VA). Plasmid DNA pX1MT-M,16 an HTLV-1 molecuFull Paper
2019Li, X;Li, X;Lin, J;Sun, X;Ding, Q;Exosomes Derived From Low-Intensity Pulsed Ultrasound-Treated Dendritic Cells Suppress Tumor Necrosis Factor-Induced Endothelial InflammationJournal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine30561085Human Umbilical Vein Endothelial Cell Culture Human umbilical vein endothelial cells were purchased from AllCells and cultured in endothelial cell medium (ScienCell, Shanghai, China) in a humidified atmosphere of 95% air and 5% CO2 at 37°CFull Paper
2019Choi, JH;Necsoiu, C;Wendorff, D;Jordan, B;Dixon, A;Roberts, TR;Beely, BM;Cancio, LC;Batchinsky, AI;Effects of adjunct treatments on end-organ damage and histological injury severity in acute respiratory distress syndrome and multiorgan failure caused by smoke inhalation injury and burnsBurns : journal of the International Society for Burn Injuries31378621The process of disqualifying AssureImmune and qualifying AllCells as a new source of bone marrow for the production of MSCs delayed our timeline. 2. Based on our published Phase SaveFull Paper
2019Ma, J;Lai, G;Lu, Z;Effect of 410 nm photodynamic therapy with hemoporfin on the expression of vascular endothelial growth factor (VEGF) in cultured human vascular endothelial cellsLasers in medical science30350123dilution with RPMI-1640 medium (ALLCELLS). Working drug solutions (0-8 μg/ml) were prepared by diluting the stock solution with RPMI-1640 medium (ALLCELLS. CA, USA). A light- SaveFull Paper
2019Miura, S;Kobune, M;Horiguchi, H;Kikuchi, S;Iyama, S;Murase, K;Goto, A;Ikeda, H;Takada, K;Miyanishi, K;Kato, J;EPO-R+ myelodysplastic cells with ring sideroblasts produce high erythroferrone levels to reduce hepcidin expression in hepatic cellsBlood cells, molecules & diseases31082798Total RNA derived from human BM CD34+, CB CD34+ and human mesenchymal stromal (MSCs) cells was purchased from AllCells, LLC (Toronto, Canada). Human myeloid leukemia SaveFull Paper
2019Li, Y;Zhao, R;Li, X;Wang, C;Bao, H;Wang, S;Fang, J;Huang, J;Wang, C;Blood-compatible Polyaniline Coated Electrospun Polyurethane Fiber Scaffolds for Enhanced Adhesion and Proliferation of Human Umbilical Vein Endothelial CellsFibers and Polymersprothrombin time agent were purchased from AllCells Biological Technology Co., Ltd. Phosphate buffered saline (PBS) was purchased from Beijing Dingguo Changsheng SaveFull Paper
2019Yamasaki, AE;King, NE;Matsui, H;Jepsen, K;Panopoulos, AD;Two iPSC lines generated from the bone marrow of a relapsed/refractory AML patient display normal karyotypes and myeloid differentiation potentialStem cell research317392017q and 13q, were obtained from AllCells, LLC. Immunohistochemistry analysis of the patient's a normal karyotype (both analyses provided by AllCells, LLC). Previously published iPSCs SaveFull Paper
2019CECHIM, G;CHIES, J;In vitro generation of human monocyte-derived dendritic cells methodological aspects in a comprehensive reviewAnais da Academia Brasileira de CiênciasCommercial leukopaks generally contain 80-200 mL of processed material with approximately 7x109peripheral blood mononuclear cells (PBMC), (more information available on: www.allcells.com/products/whole-tissue/leuko-pak). The Table I shows the percentFull Paper
2019Coronado, RE;Somaraki-Cormier, M;Ong, JL;Halff, GA;Hepatocyte-like cells derived from human amniotic epithelial, bone marrow, and adipose stromal cells display enhanced functionality when cultured on decellularized liver substrateStem cell research31163390Bone Marrow Derived Mesenchymal Stromal/Stem Cells: Bone marrow derived mesenchymal stem cells (BM-MSCs) were isolated from whole bone marrow aspirates (AllCells Alameda SaveFull Paper
2019Abhyankar, VP;Bates, AM;Fischer, CL;Johnson, GK;Guthmiller, JM;Progulske-Fox, A;Brogden, KA;Dataset on the chemokine and cytokine responses of multi-cell cultures treated with Porphyromonas gingivalis hemagglutinin BData in brief30740479HagB (QCL-1000, Chromogenic Limulus Amebocyte Lysate Assay, Lonza, Inc. Walkersville, MD USA). 2.2 Dendritic cells, GE keratinocytes, and T cells Non-peripheral blood human myeloid dendritic cells (AllCells, LLC, Alameda, CA USA) were thawed and susFull Paper
2019Hii, SC;Luddin, N;Kannan, TP;Ab Rahman, I;Nik Abdul Ghani, NR;The Biological Evaluation of Conventional and Nano-Hydroxyapatite-Silica Glass Ionomer Cement on Dental Pulp Stem Cells: A Comparative StudyContemporary clinical dentistry32308298DPSCs purchased from AllCells, USA were used in this study. DPSCs were cultured in Alpha Minimum Essential Medium (α-MEM) (Gibco, Life Technologies, USA) supplemented with SaveFull Paper
2019Akers, S;Modeling central nervous system involvement in acute lymphoblastic leukemiaWest Virginia University LibrariesScientific Corporation, Westbury, NY) according to the manufacturer’s instructions. Positively selected peripheral blood CD19+ B-cells from a healthy donor were purchased from AllCells (Emeryville, CA). Human brain-derived microvascular endothelial cFull Paper
2019Barve, A;Characterization of a more clinically relevant human leukemia xenograft model to examine perturbation of MET/SAM metabolism as a novel therapeutic paradigm for MLL-R leukemia in vivo.University of LouisvilleAcute myeloid leukemia (AML), is a heterogeneous clonal disorder characterized by an accumulation of malignant myeloid progenitors in the bone marrow (BM), hindering normal SaveFull Paper
2019Liu, H;Kezios, S;DYNAMIC CHARACTERISTICS OF ETTINGSHAUSEN REFRIGERATORProceeding of International Heat Transfer Conference 4However this was not the case in allcells and in cells from plant 41, with 3 accessory chromo¬somes, sometimes none, one, two or all three accessorychromosomes divided precociously SaveFull Paper
2019Rodriguez, L;Mohammadipoor, A;Kamucheka, R;Asher, A;Alvarado, L;Antebi, B;Cancio, L;Preconditioning in an Acute Respiratory Distress Syndrome-Like Milieu Augments the Immunomodulatory and Anti-Inflammatory Function of MSCSA60. LUNG INJURY, SEPSIS, AND ARDSMethods: Human bone marrow (BM)-MSCs were isolated from fresh mononuclear cells purchased from AllCells (Alameda, CA). Human adipose-derived (AD)-MSCs were isolated from SaveFull Paper
2019De Maio, V;Brandic, I;Multi-Objective Mobile Edge Provisioning in Small Cell CloudsProceedings of the 2019 ACM/SPEC International Conference on Performance EngineeringAlso, we can observe significant losses when using the other two algorithms, ALLCELLS and RANDOM. In the first case this is because the algorithm overestimates the number of Edge SaveFull Paper
2019Lal, A;Chiang, Z;Yakovenko, N;Duarte, F;Israeli, J;Buenrostro, J;AtacWorks: A deep convolutional neural network toolkit for epigenomicsbioRxivCryopreserved human bone marrow mononuclear cells were purchased from Allcells (catalog number BM, CR, MNC, 10M). Cells were quickly thawed in a 37°C water bath, rinsed with culture medium (RPMI 1640 medium supplemented with 15% FBS) andFull Paper
2019Liu, J;Kozhaya, L;Torres, V;Unutmaz, D;Lu, M;Structure-Based Discovery of a Novel Small-Molecule Inhibitor of Methicillin-Resistant S. aureusbioRxivBlood samples were obtained from healthy, consenting donors as Buffy coats (New York Blood Center) and leukopaks (AllCells, Alameda, CA). PBMCs were isolated from peripheral blood by density gradient centrifugation using Ficoll-PaqueFull Paper
2019Wang, X;Derakhshandeh, R;Rodriguez, H;Han, D;Kostyushev, D;Henry, T;Traverse, J;Moyé, L;Simari, R;Taylor, D;Springer, M;Impaired Therapeutic Efficacy of Bone Marrow Cells from Post-Myocardial Infarction Patients in the TIME and LateTIME Clinical TrialsbioRxivHealthy de-identified donor BM MNCs were purchased from AllCells (Alameda, CA). These MNCs were manually isolated at AllCells using Ficoll conditions like that in the Sepax SaveFull Paper
2019Granja, J;Klemm, S;McGinnis, L;Kathiria, A;Mezger, A;Parks, B;Gars, E;Liedtke, M;Zheng, G;Chang, H;Majeti, R;Greenleaf, W;A single cell framework for multi-omic analysis of disease identifies malignant regulatory signatures in mixed phenotype acute leukemiabioRxivPBMCs, BMMCs, and CD34+ bone marrow cells were obtained from healthy donors (AllCells). Description of Leukemic Patients/Donors Patient samples were collected with informed consent prospectively under a protocol approved by the Institutional ReviewFull Paper
2019Ding, J;Adiconis, X;Simmons, S;Kowalczyk, M;Hession, C;Marjanovic, N;Hughes, T;Wadsworth, M;Burks, T;Nguyen, L;Kwon, J;Barak, B;Ge, W;Kedaigle, A;Carroll, S;Li, S;Hacohen, N;Rozenblatt-Rosen, O;Shalek, A;Villani, A;Regev, A;Levin, J;Systematic comparative analysis of single cell RNA-sequencing methodsbioRxivWe acquired commercially-available PBMCs (AllCells; Lot #PB006F-A5865) as frozen aliquots with 25 million cells for the PBMC1 experiment or isolated them from fresh blood (AllCells SaveFull Paper
2019Fan, L;Zhao, M;Gold nanorod based delivery system could bring about superior therapy effect of Ramucirumab through direct cytotoxicity to cancer cell mediated by differential regulation of phagocytosis in gastric cancer cellbioRxivHUVEC cell was purchased from Allcells (Endothelial Cell Medium, H-004B, Allcells, supplemented with 5%fetal bovine serum), GC cells SNU-5 (1640 Cell Medium, Gibco, supplemented with 10% fetal bovine serum) cell was purchased from ATCC and MKNFull Paper
2019King, GR;Comparative systemic analysis of human immunoglobulin repertoiresThesisAll peripheral blood and bone marrow aspirate samples were collected by AllCells and shipped overnight at ambient temperature; processing was started immediately upon receipt of the samples in a sterilized biological safety hood. Sterile 1x PBS pH 7.Full Paper
2019Sakhnevych, S;Tim-3-galectin-9 immunosuppressive pathway in human acute myeloid leukaemia and solid tumour cells and biochemical functions of its crucial componentsThesisPrimary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordance with the manufacturer's instructions following ethical approval (REC reference: 16-SS-033). Other pFull Paper
2019Lee, MJ;The Development of Novel Proteasome Inhibitors for the Treatment of Multiple Myeloma and Alzheimer's DiseaseThesisOver a decade, proteasome inhibitors (PIs), bortezomib, carfilzomib (Cfz) and ixazomib, have contributed to a significant improvement in the overall survival for multiple myeloma (MM) SaveFull Paper
2019Kuhlman, BM;MICROGRAVITY NEGATIVELY AFFECTS DNA DAMAGE REPAIR AND THE ANTI-LEUKEMIC FUNCTION OF HUMAN NATURAL KILLER CELLSThesisHealthy human bone marrow (BM) mononuclear cells (BMMNC) were purchased from AllCells, LLC (Alameda, CA, USA). CD34+ HSC were isolated from BMMNC using antihuman CD34 MicroBeads and the MiniMACS magnetic-based positive-selection system (Miltenyi BiotFull Paper
2019龚子祯, ;蔡海波, ;谭文松, ;黄原胶支持 CIK 细胞高密度扩增高校化学工程学报RPMI 1640购于美国Gibco公司; 血清培养基(自配);PBMC购于上海Allcells公司;CD3单克隆 抗体购于美国eBioscience公司;植物血凝 PHA购于美国Sigma-Aldrich公司;干扰 (IFN-γ),白介 SaveFull Paper
2019Cao, YP;Pan, M;Song, YL;Zhang, HL;MiR-302 a/b/c suppresses tumor angiogenesis in hepatocellular carcinoma by targeting MACC1European Review for Medical and Pharmacological SciencesThe umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Huh7 and PLC/PRF/5 cells were incubated with Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serFull Paper
2019王一唯, ;黄婷, ;孙笑, ;王玉东, ;RANKL 募集调节性 T 细胞促进子宫内膜癌侵袭的研究中国免疫学杂志科院细胞库),外周单个 细胞(美国ALLCELLS 公 司),RANK 慢病毒(上海和元生物科技公司),重组 人TGF鄄茁,重组人RANKL(美国Peprotech 公司),人 RANKL ELISA 试剂盒(武汉博士德生物 SaveFull Paper
2019Alvarado, L;Evaluating the Therapeutic Effects of Mesenchymal Stem Cells in an in Vitro Co-Culture System of a Damaged Alveolar BarrierThesisHuman BM-MSCs were isolated from commercially available MNCs (AllCells LLC; Emeryville, CA). Bone marrow specimen were obtained from human donors 20-30 years of age. MNCs were cultured on standard culture flasks at a density of 3 x 105 cells/cm2 inFull Paper
2019Romero-Masters, J;Studying Epstein-Barr virus-induced lymphomagenesis in a cord-blood humanized mouse modelThesisCreation of cord blood-humanized NOD/LtSz-scid/IL2Rγnull mice: Immunodeficient NSG (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson labs (catalogue number: 005557). Commercially available CD34-depleted human cord blood mononuclear cells (AlFull Paper
2019Mumbach, MR;Technologies for Protein and RNA-Focused Chromosome ConformationThesisNormal donor human peripheral blood cells were obtained fresh from AllCells. CD4+ T cells were enriched from peripheral blood using the RosetteSep Human CD4+ T Cell Enrichment Cocktail (StemCell Technology). For CD4+ T helper cell subtypes, Naïve T cFull Paper
2019Walker, KL;Non-chemotherapeutic Methods of Treating T Cell Acute Lymphoblastic LeukemiaThesisChapter 4.2: EOpansion of NK cell products Fresh leukopaks were commercially o9tained (AllCells, Alameda, CA) from paid human apheresis donors, stored at 1Г10o C, and SaveFull Paper
2019Srivastava, S;Salter, AI;Liggitt, D;Yechan-Gunja, S;Sarvothama, M;Cooper, K;Smythe, KS;Dudakov, JA;Pierce, RH;Rader, C;Riddell, SR;Logic-Gated ROR1 Chimeric Antigen Receptor Expression Rescues T Cell-Mediated Toxicity to Normal Tissues and Enables Selective Tumor TargetingCancer cell30889382CFU Assays Femurs and tibia from control and ROR1 CAR-T cell-treated mice were harvested 9 days post-T cell transfer and submitted to ReachBio Inc. for CFU-GM, BFU-E, CFU-Mk, Pre-B CFC, and CFU-F assaysFull Paper
2019Humbert, O;Radtke, S;Samuelson, C;Carrillo, RR;Perez, AM;Reddy, SS;Lux, C;Pattabhi, S;Schefter, LE;Negre, O;Lee, CM;Bao, G;Adair, JE;Peterson, CW;Rawlings, DJ;Scharenberg, AM;Kiem, HP;Therapeutically relevant engraftment of a CRISPR-Cas9-edited HSC-enriched population with HbF reactivation in nonhuman primatesScience translational medicine31366580For CFC assays, 1000-1200 sorted cells were seeded into 3.5 ml ColonyGEL 1402 (ReachBio). Hematopoietic colonies were scored after 12-14 days. Arising colonies were identified as colony-forming unit- (CFU-) granulocyte (CFU-G), macrophageFull Paper
2019Crawford, A;Haber, L;Kelly, MP;Vazzana, K;Canova, L;Ram, P;Pawashe, A;Finney, J;Jalal, S;Chiu, D;Colleton, CA;Garnova, E;Makonnen, S;Hickey, C;Krueger, P;DelFino, F;Potocky, T;Kuhnert, J;Godin, S;Retter, MW;Duramad, P;MacDonald, D;Olson, WC;Fairhurst, J;Huang, T;Martin, J;Lin, JC;Smith, E;Thurston, G;Kirshner, JR;A Mucin 16 bispecific T cell-engaging antibody for the treatment of ovarian cancerScience translational medicine31217340Regeneron Pharmaceuticals Institutional Animal Care and Use Committee. NSG (20 to 24 weeks old) mice (the Jackson Laboratory) were injected with 5 × 106 human PBMCs (ReachBio) before 2 × 106 ascites cells from the OVCAR-3/Luc cell line,Full Paper
2019Li, C;Mishra, AS;Gil, S;Wang, M;Georgakopoulou, A;Papayannopoulou, T;Hawkins, RD;Lieber, A;Targeted Integration and High-Level Transgene Expression in AAVS1 Transgenic Mice after In Vivo HSC Transduction with HDAd5/35++ VectorsMolecular therapy : the journal of the American Society of Gene Therapy314940532,500 Lin− cells were plated in triplicates in ColonyGEL 1202 mouse complete medium (ReachBio, Seattle, WA) and incubated for 12 days at 37°C in 5% CO2 and maximum humidity. Colonies were enumerated using a Leica MS 5 dissection microscope (Leica MicFull Paper
2019Kuhlmann, AS;Haworth, KG;Barber-Axthelm, IM;Ironside, C;Giese, MA;Peterson, CW;Kiem, HP;Long-Term Persistence of Anti-HIV Broadly Neutralizing Antibody-Secreting Hematopoietic Cells in Humanized MiceMolecular therapy : the journal of the American Society of Gene Therapy30391142erythropoietin (EPO), granulocyte colony-stimulating factor (G-CSF), and granulocyte-macrophage colony-stimulating factor (GM-CSF). At necropsy, cells isolated from bone marrow (BM) were seeded in triplicate into ColonyGEL (ReachBio, Seattle WA). HemFull Paper
2019Humbert, O;Laszlo, GS;Sichel, S;Ironside, C;Haworth, KG;Bates, OM;Beddoe, ME;Carrillo, RR;Kiem, HP;Walter, RB;Engineering resistance to CD33-targeted immunotherapy in normal hematopoiesis by CRISPR/Cas9-deletion of CD33 exon 2Leukemia30291334system (Harvard Apparatus, Cambridge, MA) [12]. For evaluation of colony-forming units (CFUs), 1500 CD34+ cells were seeded in 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA) and scored after 12-14 days. CFU DNA was extracted in QuickExtract (EpicentreFull Paper
2019Ly, M;Rentas, S;Vujovic, A;Wong, N;Moreira, S;Xu, J;Holzapfel, N;Bhatia, S;Tran, D;Minden, MD;Draper, JS;Hope, KJ;Diminished AHR Signaling Drives Human Acute Myeloid Leukemia Stem Cell MaintenanceCancer research31519687Thawed primary AML samples were counted and plated in a methylcellulose-based hematopoietic colony formation medium (Colony Gel, ReachBio), supplemented with DMSO or 750 SaveFull Paper
2019Smitheman, KN;Severson, TM;Rajapurkar, SR;McCabe, MT;Karpinich, N;Foley, J;Pappalardi, MB;Hughes, A;Halsey, W;Thomas, E;Traini, C;Federowicz, KE;Laraio, J;Mobegi, F;Ferron-Brady, G;Prinjha, RK;Carpenter, CL;Kruger, RG;Wessels, L;Mohammad, HP;Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypesHaematologica30514804uated for each assay. Primary acute myeloid leukemia blast colony Clonogenic progenitors from primary AML patients’ samples were assessed in a semi-solid methylcellulose-based medium (ColonyGel , ReachBio) containing recombinant human (rh) SCF (50 ngFull Paper
2019Fuller, NO;Pirone, A;Lynch, BA;Hewitt, MC;Quinton, MS;McKee, TD;Ivarsson, M;CoREST Complex-Selective Histone Deacetylase Inhibitors Show Prosynaptic Effects and an Improved Safety Profile To Enable Treatment of SynaptopathiesACS chemical neuroscience30496686Normal human bone marrow light density cells derived from normal bone marrow (NorCal Biologics, California) and qualified at ReachBio were stored in the gaseous phase of liquid Save;minimum peptide length of at least 7 amino acids was required. EfFull Paper
2019Decker, CE;Young, T;Pasnikowski, E;Chiu, J;Song, H;Wei, Y;Thurston, G;Daly, C;Genome-scale CRISPR activation screen uncovers tumor-intrinsic modulators of CD3 bispecific antibody efficacyScientific reports31882897Healthy donor peripheral blood mononuclear cells (PBMCs) were purchased from ReachBio Research Labs. T cells were isolated by negative selection using the Dynabeads Save;Clec12axCD3 bsAbs were isolated by selective protein A affinity chromatographyFull Paper
2019Ostrakhovitch, EA;Akakura, S;Sanokawa-Akakura, R;Tabibzadeh, S;3-Mercaptopyruvate sulfurtransferase disruption in dermal fibroblasts facilitates adipogenic trans-differentiationExperimental cell research31634482Human dermal fibroblasts (HDF) were obtained from ATCC (Manassas, VA). Mesenchymal stem cells (MSCs), isolated from a 25-year-old male donor, were purchased from ReachBio Research lab (Seattle, WA). Chemicals were from Sigma-Aldrich Company (St LouisFull Paper
2019Radtke, S;Chan, YY;Sippel, TR;Kiem, HP;Rongvaux, A;MISTRG mice support engraftment and assessment of nonhuman primate hematopoietic stem and progenitor cellsExperimental hematology30590092For CFC assays, 1000-1200 sort-purified NHP or CD34-subpopulations were seeded into 3.5 mL ColonyGEL 1402 (ReachBio, Seattle, WA). Hematopoietic colonies were scored after 12-14 days. Arising colonies were identified as colony-forming unit- (CFU-) grFull Paper
2019Radtke, S;Perez, AM;Venkataraman, R;Reddy, S;Haworth, KG;Humbert, O;Kiem, HP;Peterson, CW;Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor CellsJournal of visualized experiments : JoVE30829324Protamine sulfate Sigma P-4505 14 mL Polypropylene Round-Bottom Tube Corning 352059 Colony Gel 1402 ReachBio 1402 QuadroMACS Separators Miltenyi Biotec 130-090-976Full Paper
2018Cristescu, R;Mogg, R;Ayers, M;Albright, A;Murphy, E;Yearley, J;Sher, X;Liu, XQ;Lu, H;Nebozhyn, M;Zhang, C;Lunceford, JK;Joe, A;Cheng, J;Webber, AL;Ibrahim, N;Plimack, ER;Ott, PA;Seiwert, TY;Ribas, A;McClanahan, TK;Tomassini, JE;Loboda, A;Kaufman, D;Pan-tumor genomic biomarkers for PD-1 checkpoint blockade-based immunotherapyScience30309915Associations of TMB and the T cell–inflamed GEP with BOR and PFS were evaluated by using tumor samples from subgroups of patients treated with pembrolizumab in clinical trials who had WES data available. These included a discovery cohort of patientsFull Paper
2018Smith, S;Lynch, R;Till, B;Cowan, A;Wu, Q;Voutsinas, J;Shadman, M;Blue, K;Shustov, A;Kanan, S;Low, L;Cassaday, R;Fromm, J;Gopal, A;Pembrolizumab in Combination with Standard RCHOP Therapy for Previously Untreated Diffuse Large B-Cell LymphomaBloodSecondary endpoints included response rates, overall (OS) and progression-free survival (PFS). Baseline PDL1 expression was analyzed centrally (Merck/Qualtek). Peripheral blood flow cytometry for changes in PD1, PDL-1, and PDL-2 subsets were performeFull Paper
2018Doi, T;Piha-Paul, SA;Jalal, SI;Saraf, S;Lunceford, J;Koshiji, M;Bennouna, J;Safety and Antitumor Activity of the Anti-Programmed Death-1 Antibody Pembrolizumab in Patients With Advanced Esophageal CarcinomaJ. Clin. Oncol.29116900PD-L1 Expression Analysis PD-L1 expression was assessed at screening by a central laboratory on the basis of a laboratory-developed prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA) using either an archived (formalin-fiFull Paper
2018Kanjanapan, Y;Lien, S;Yang, C;Clouthier, D;Elston, S;Jiang, H;Butler, M;Hogg, D;Ohashi, P;Pugh, T;Siu, L;Spreafico, A;Genomic and immune landscape of metastatic melanoma (MM) treated with pembrolizumab (PEM).JCOBaseline (BL) and on treatment (post 2-3 doses of PEM) fresh tumor biopsies were collected for DNA/RNA sequencing, immune-profiling, and tumor PD-L1 expression by immunohistochemistry (QualTek), with longitudinal blood collection for immunophenotypiFull Paper
2018Graff, J;Alumkal, J;Thompson, R;Moran, A;Thomas, G;Wood, M;Drake, C;Slottke, R;Beer, T;Pembrolizumab (Pembro) plus enzalutamide (Enz) in metastatic castration resistant prostate cancer (mCRPC): Extended follow up.JCOThe other 2 had neither. Of the NR who had sequencing, 4 had a DNA repair defects, and none had MSI. None of the biopsies showed tumoral PD-L1 expression (Qualtek, 22C3). Conclusions: Pembro has activity in mCRPC when added to EnzFull Paper
2018Kelly, C;Bowler, T;Munhoz, R;Chi, P;Dickson, M;Gounder, M;Keohan, M;Dholakia, R;Condy, M;Singer, S;Crago, A;Yoon, S;Ariyan, C;Hwang, S;Erinjeri, J;Antonescu, C;Qin, L;Tap, W;D'Angelo, S;A phase II study of talimogene laherparepvec (T-VEC) and pembrolizumab in patients with metastatic sarcoma.JCOoverall survival. Correlative studies included PD-L1 expression by IHC (Qualtek), multiplex IHC, characterization of tumor infiltrating lymphocytes, mutational burden/neoantigen analysis, and T cell receptor clonality. ResultsFull Paper
2018Smith, JJ;Timoshevskaya, N;Ye, C;Holt, C;Keinath, MC;Parker, HJ;Cook, ME;Hess, JE;Narum, SR;Lamanna, F;Kaessmann, H;Timoshevskiy, VA;Waterbury, CKM;Saraceno, C;Wiedemann, LM;Robb, SMC;Baker, C;Eichler, EE;Hockman, D;Sauka-Spengler, T;Yandell, M;Krumlauf, R;Elgar, G;Amemiya, CT;The sea lamprey germline genome provides insights into programmed genome rearrangement and vertebrate evolutionNat. Genet.29358652Sequencing. Fragment libraries were prepared by Covaris shearing of sperm genomic DNA isolated from a single individual and size selected to achieve average insert sizes of ~205 and 231bp. These libraries were sequenced on the Illumina HiSeq2000 platFull Paper
2018D'Angelo, SP;Melchiori, L;Merchant, MS;Bernstein, D;Glod, J;Kaplan, R;Grupp, S;Tap, WD;Chagin, K;Binder, GK;Basu, S;Lowther, DE;Wang, R;Bath, N;Tipping, A;Betts, G;Ramachandran, I;Navenot, JM;Zhang, H;Wells, DK;Van Winkle, E;Kari, G;Trivedi, T;Holdich, T;Pandite, L;Amado, R;Mackall, CL;Antitumor Activity Associated with Prolonged Persistence of Adoptively Transferred NY-ESO-1 c259T Cells in Synovial SarcomaCancer Discov29891538Serial 5-μm sections of formalin-fixed, paraffin-embedded tissues were analyzed for the following single markers by IHC: NY-ESO-1, Pan-CK (Clone AE1/AE3/PCK26, Ventana), CD45 (Clone 2B11 + PD7/26, Ventana), CD3 (Clone 2GV6, Ventana), CD8 (Clone C8/14Full Paper
2018Dejanovic, B;Huntley, MA;De Mazière, A;Meilandt, WJ;Wu, T;Srinivasan, K;Jiang, Z;Gandham, V;Friedman, BA;Ngu, H;Foreman, O;Carano, RAD;Chih, B;Klumperman, J;Bakalarski, C;Hanson, JE;Sheng, M;Changes in the Synaptic Proteome in Tauopathy and Rescue of Tau-Induced Synapse Loss by C1q AntibodiesNeuron30392797Biological Samples Human brain tissue Folio Biosciences N/AFull Paper
2018Schafer, PH;Ye, Y;Wu, L;Kosek, J;Ringheim, G;Yang, Z;Liu, L;Thomas, M;Palmisano, M;Chopra, R;Cereblon modulator iberdomide induces degradation of the transcription factors Ikaros and Aiolos: immunomodulation in healthy volunteers and relevance to systemic lupus erythematosusAnn. Rheum. Dis.29945920study in healthy volunteers. Methods. Gene expression studies. Viably frozen PBMCs from 11 patients with SLE and 10 healthy volunteers were obtained from Conversant Bio (Huntsville, Alabama, USA). Informed consent wasFull Paper
2018Liles, JT;Corkey, BK;Notte, GT;Budas, GR;Lansdon, EB;Hinojosa-Kirschenbaum, F;Badal, SS;Lee, M;Schultz, BE;Wise, S;Pendem, S;Graupe, M;Castonguay, L;Koch, KA;Wong, MH;Papalia, GA;French, DM;Sullivan, T;Huntzicker, EG;Ma, FY;Nikolic-Paterson, DJ;Altuhaifi, T;Yang, H;Fogo, AB;Breckenridge, DG;ASK1 contributes to fibrosis and dysfunction in models of kidney diseaseJ. Clin. Invest.30024858... NAL BIOPSIES AND DB/DB ENOS–/– MOUSE KIDNEYS Formalin-fixed paraffin-embedded renal biopsies from 10 patients with DKD and from 7 donors without kidney disease (“healthy”) were obtained from Folio Biosciences. Kidney sections were prepared fromFull Paper
2018Gadgeel, SM;Pennell, NA;Fidler, MJ;Halmos, B;Bonomi, P;Stevenson, J;Schneider, B;Sukari, A;Ventimiglia, J;Chen, W;Galasso, C;Wozniak, A;Boerner, J;Kalemkerian, GP;Phase II Study of Maintenance Pembrolizumab in Patients with Extensive-Stage Small Cell Lung Cancer (SCLC)J Thorac Oncol29775808than every four cycles. Retrieval of pretreatment biopsy specimens for assessment of PD-L1 expression in tumor cells and stromal tissue was conducted by Qualtek Clinical Laboratories (Newton, PA). Assessment of tumor PDFull Paper
2018Roberts, BS;Hardigan, AA;Moore, DE;Ramaker, RC;Jones, AL;Fitz-Gerald, MB;Cooper, GM;Wilcox, CM;Kimberly, RP;Myers, RM;Discovery and Validation of Circulating Biomarkers of Colorectal Adenoma by High-Depth Small RNA SequencingClin. Cancer Res.29490987HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.Full Paper
2018Tucker, DW;Getchell, CR;McCarthy, ET;Ohman, AW;Sasamoto, N;Xu, S;Ko, JY;Gupta, M;Shafrir, A;Medina, JE;Lee, JJ;MacDonald, LA;Malik, A;Hasselblatt, KT;Li, W;Zhang, H;Kaplan, SJ;Murphy, GF;Hirsch, MS;Liu, JF;Matulonis, UA;Terry, KL;Lian, CG;Dinulescu, DM;Epigenetic Reprogramming Strategies to Reverse Global Loss of 5-Hydroxymethylcytosine, a Prognostic Factor for Poor Survival in High-grade Serous Ovarian CancerClin. Cancer Res.29263182... s of 5-hmC levels in a large biorepository of HGSOC cases by using a patient tumor microarray (TMA) from the Dana-Farber Cancer Institute (DFCI, Boston, MA; ref. 22) and a commercially available TMA (Folio Biosciences). We observed strong 5-hmC sFull Paper
2018Subbiah, V;Murthy, R;Hong, DS;Prins, RM;Hosing, C;Hendricks, K;Kolli, D;Noffsinger, L;Brown, R;McGuire, M;Fu, S;Piha-Paul, S;Naing, A;Conley, AP;Benjamin, RS;Kaur, I;Bosch, ML;Cytokines Produced by Dendritic Cells Administered Intratumorally Correlate with Clinical Outcome in Patients with Diverse CancersClin. Cancer Res.30018119Biopsied tumors were formalin fixed and paraffin embedded (FFPE) using standard methods. All immunohistochemistry was performed by QualTek Molecular Laboratories (Santa Barbara, CA). In situ detection of IFNγ and TNFα transcripts in FFPE specimens wFull Paper
2018Joseph, RW;Elassaiss-Schaap, J;Kefford, R;Hwu, WJ;Wolchok, JD;Joshua, AM;Ribas, A;Hodi, FS;Hamid, O;Robert, C;Daud, A;Dronca, R;Hersey, P;Weber, JS;Patnaik, A;de Alwis, DP;Perrone, A;Zhang, J;Kang, SP;Ebbinghaus, S;Anderson, KM;Gangadhar, TC;Baseline Tumor Size Is an Independent Prognostic Factor for Overall Survival in Patients with Melanoma Treated with PembrolizumabClin. Cancer Res.29685882Tumor PD-L1 expression was assessed by a prototype IHC assay (QualTek Molecular Laboratories; ref. 14) in pretreatment tumor biopsy samples using the 22C3 antibody (Merck & Co., Inc.). PD-L1 positivity was defined as membranous staining in 1% of tumoFull Paper
2018Rugo, HS;Delord, JP;Im, SA;Ott, PA;Piha-Paul, SA;Bedard, PL;Sachdev, J;Le Tourneau, C;van Brummelen, EMJ;Varga, A;Salgado, R;Loi, S;Saraf, S;Pietrangelo, D;Karantza, V;Tan, AR;Safety and Antitumor Activity of Pembrolizumab in Patients with Estrogen Receptor-Positive/Human Epidermal Growth Factor Receptor 2-Negative Advanced Breast CancerClin. Cancer Res.29559561An archival or newly obtained core or excisional biopsy specimen from a nonirradiated tumor lesion was collected from each patient for assessment of PD-L1 expression. Tumor PD-L1 expression was assessed by immunohistochemistry at a central laboratoryFull Paper
2018Thompson, ML;Finnila, CR;Bowling, KM;Brothers, KB;Neu, MB;Amaral, MD;Hiatt, SM;East, KM;Gray, DE;Lawlor, JMJ;Kelley, WV;Lose, EJ;Rich, CA;Simmons, S;Levy, SE;Myers, RM;Barsh, GS;Bebin, EM;Cooper, GM;Genomic sequencing identifies secondary findings in a cohort of parent study participantsGenet. Med.29790872to change their preferences at the return of results appointment. Whole exome and genome sequencing Blood samples were sent for sequencing at the HudsonAlpha Genomic Services Laboratory (http://gsl.hudsonalpha.org). Genomic DNA was isolated from periFull Paper
2018Pena, LDM;Jiang, YH;Schoch, K;Spillmann, RC;Walley, N;Stong, N;Rapisardo Horn, S;Sullivan, JA;McConkie-Rosell, A;Kansagra, S;Smith, EC;El-Dairi, M;Bellet, J;Keels, MA;Jasien, J;Kranz, PG;Noel, R;Nagaraj, SK;Lark, RK;Wechsler, DSG;Del Gaudio, D;Leung, ML;Hendon, LG;Parker, CC;Jones, KL;, ;Goldstein, DB;Shashi, V;Looking beyond the exome: a phenotype-first approach to molecular diagnostic resolution in rare and undiagnosed diseasesGenet. Med.28914269Kimberly Splinter Harvard University (CC) David P. Bick HudsonAlpha Camille L. Birch HudsonAlpha Braden E. Boone HudsonAlpha Donna M. Brown HudsonAlpha Daniel C. Dorset HudsonAlpha Lori H. Handley HudsonAlpha Howard J. Jacob HudsonAlpha Angela L. JoFull Paper
2018Abraham, B;Pharma, GUCB;Sankyo, HD;Disclosure of relevant financial relationshipsJournal of the American Academy of DermatologyAbramovits, William; Grants - AbbVie, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant Bio, Dermavant Abramovits, William; Honoraria - AbbVie, Allergan, Amgen, Anacor Pharm, Aqua Pharma, Celgene, Centocor, Conversant Bio, DFull Paper
2018Menard, LC;Fischer, P;Kakrecha, B;Linsley, PS;Wambre, E;Liu, MC;Rust, BJ;Lee, D;Penhallow, B;Manjarrez Orduno, N;Nadler, SG;Renal Cell Carcinoma (RCC) Tumors Display Large Expansion of Double Positive (DP) CD4+CD8+ T Cells With Expression of Exhaustion MarkersFront Immunol30534127Samples for the discovery and replication cohorts were provided by Folio-Conversant Bio (AL), MT Group (CA), Rutgers-CINJ or the Benaroya Research Institute. All patients gave written informed consent at time of sample collection according to IRB proFull Paper
2018Tisdale, J;Kanter, J;Mapara, M;Kwiatkowski, J;Krishnamurti, L;Schmidt, M;Miller, A;Pierciey, F;Shi, W;Ribeil, J;Asmal, M;Thompson, A;Walters, M;Current Results of Lentiglobin Gene Therapy in Patients with Severe Sickle Cell Disease Treated Under a Refined Protocol in the Phase 1 Hgb-206 StudyBloodBackground β-globin gene transfer has the potential for substantial clinical benefit in patients with sickle cell disease (SCD). LentiGlobin Drug Product (DP) contains autologous CD34+ hematopoietic stem cells (HSCs) transduced with thFull Paper
2018Klemann, CJHM;Xicoy, H;Poelmans, G;Bloem, BR;Martens, GJM;Visser, JE;Physical Exercise Modulates L-DOPA-Regulated Molecular Pathways in the MPTP Mouse Model of Parkinson's DiseaseMol. Neurobiol.29019056pooled for RNAseq analysis. RNA Sequencing and Data Processing. All RNA samples were subjected to RNA sequencing (RNAseq; HudsonAlpha Genomic Services Lab, Huntsville, AL) as performed before [37]. In short, totalFull Paper
2018Van Laar, R;Lincoln, M;Van Laar, B;Development and validation of a plasma-based melanoma biomarker suitable for clinical useBr. J. Cancer29360813expression profiles of individuals with and without melanoma, 0.5-2 ml of cell-free plasma was obtained from 16 healthy controls and 32 Caucasian individuals at the time of their diagnosis with cutaneous melanoma (Cureline Inc., CA, USA and Folio BioFull Paper
2018Buatois, V;Johnson, Z;Salgado-Pires, S;Papaioannou, A;Hatterer, E;Chauchet, X;Richard, F;Barba, L;Daubeuf, B;Cons, L;Broyer, L;D'Asaro, M;Matthes, T;LeGallou, S;Fest, T;Tarte, K;Clarke Hinojosa, RK;Genescà Ferrer, E;Ribera, JM;Dey, A;Bailey, K;Fielding, AK;Eissenberg, L;Ritchey, J;Rettig, M;DiPersio, JF;Kosco-Vilbois, MH;Masternak, K;Fischer, N;Shang, L;Ferlin, WG;Preclinical Development of a Bispecific Antibody that Safely and Effectively Targets CD19 and CD47 for the Treatment of B-Cell Lymphoma and LeukemiaMol. Cancer Ther.29743205Cells were cultured at 37°C and 5% CO2. Primary samples were collected from Conversant Biologics (Huntsville, USA), the Leukemia Research Center (Glasgow, UK), the Geneva University Hospital (Geneva, Switzerland) ;... tometry and finally selecteFull Paper
2018Foulk, B;Schaffer, M;Gross, S;Rao, C;Smirnov, D;Connelly, MC;Chaturvedi, S;Reddy, M;Brittingham, G;Mata, M;Repollet, M;Rojas, C;Auclair, D;DeRome, M;, ;Weiss, B;Sasser, AK;Enumeration and characterization of circulating multiple myeloma cells in patients with plasma cell disordersBr. J. Haematol.29105742Verification cohort samples were obtained from Conversant Biologics (Huntsville, AL, USA) under a biospecimen purchase agreement. These samples represent a heterogenous cohort of patients with active disease and those in remissionFull Paper
2018Pekcec, A;Schülert, N;Stierstorfer, B;Deiana, S;Dorner-Ciossek, C;Rosenbrock, H;Targeting the dopamine D1 receptor or its downstream signalling by inhibiting phosphodiesterase-1 improves cognitive performanceBr. J. Pharmacol.29726015paraffin blocks. Human brain tissue from healthy donors (Folio Biosciences, Ohio, USA) was fixed in 4% paraformaldehyde, and samples containing PFC were identified, trimmed and processed into paraffin blocks. All procurementFull Paper
2018Maity, A;Mick, R;Huang, AC;George, SM;Farwell, MD;Lukens, JN;Berman, AT;Mitchell, TC;Bauml, J;Schuchter, LM;O'Hara, M;Lin, LL;Demichele, A;Christodouleas, JP;Haas, NB;Patsch, DM;Hahn, SM;Minn, AJ;Wherry, EJ;Vonderheide, RH;A phase I trial of pembrolizumab with hypofractionated radiotherapy in patients with metastatic solid tumoursBr. J. Cancer30318516ange in volume of the irradiated (index) lesion was also calculated, but this was not used in the RECIST measurement. Immunohistochemical staining PD-L1 staining was performed by an outside company (QualTek Molecular Laboratories, Newtown, PA, USA)Full Paper
2018Mehra, R;Seiwert, TY;Gupta, S;Weiss, J;Gluck, I;Eder, JP;Burtness, B;Tahara, M;Keam, B;Kang, H;Muro, K;Geva, R;Chung, HC;Lin, CC;Aurora-Garg, D;Ray, A;Pathiraja, K;Cheng, J;Chow, LQM;Haddad, R;Efficacy and safety of pembrolizumab in recurrent/metastatic head and neck squamous cell carcinoma: pooled analyses after long-term follow-up in KEYNOTE-012Br. J. Cancer29955135PD-L1 expression status during screening was determined using a prototype PD-L1 immunohistochemical assay 18 performed at a laboratory site (QualTek) accredited by the College of American Pathologists and Clinical Laboratory Improvement Amendments, aFull Paper
2018Wein, L;Luen, SJ;Savas, P;Salgado, R;Loi, S;Checkpoint blockade in the treatment of breast cancer: current status and future directionsBr. J. Cancer29808015Patients were required to be PD-L1 positive by immunohistochemistry (IHC), which was defined as PD-L1 positivity in the stroma or ≥1% tumour cells using their Qualtek assay.28 Most patients were heavily pre-treated; the median number of previous lineFull Paper
2018Kanter, J;Tisdale, J;Kwiatkowski, J;Krishnamurti, L;Mapara, M;Schmidt, M;Miller, A;Pierciey, F;Shi, W;Ribeil, J;Walters, M;Thompson, A;Outcomes for Initial Patient Cohorts with up to 33 Months of Follow-up in the Hgb-206 Phase 1 TrialBloodBackground Sickle cell disease (SCD) is a progressively debilitating genetic disease causing significant morbidity and early mortality for which a universal curative therapy is lacking. Expression of an anti-sickling β-globin via geneFull Paper
2018Bosco, EE;Christie, RJ;Carrasco, R;Sabol, D;Zha, J;DaCosta, K;Brown, L;Kennedy, M;Meekin, J;Phipps, S;Ayriss, J;Du, Q;Bezabeh, B;Chowdhury, P;Breen, S;Chen, C;Reed, M;Hinrichs, M;Zhong, H;Xiao, Z;Dixit, R;Herbst, R;Tice, DA;Preclinical evaluation of a GFRA1 targeted antibody-drug conjugate in breast cancerOncotarget29796165... Fisher Scientific, Waltham, MA, USA) were coated with 5 mg/ml 9B3 antibody at 4○C overnight, washed, and blocked with 1% bovine serum albumin (Sigma-Aldrich, St Louis, MO). Human serum samples from Conversant Bio (Huntsville, AL) or from cell-linFull Paper
2018Xu, Z;Wang, S;Li, Y;Zhu, F;Huang, J;PRIM: An Efficient Preconditioning Iterative Reweighted Least Squares Method for Parallel Brain MRI ReconstructionNeuroinformatics29423650International Holdings Inc. Albin, Mary, Westinghouse Alexander, William, Qualtek Manufacturing, Inc. Alexander, Chuck, Solid Concepts, Inc. Alexopoulos, Nick, Broadcom Corporation Alford, Charles, UES, Inc. Alvarado, IgorFull Paper
2018Locatelli, F;Walters, M;Kwiatkowski, J;Porter, J;Sauer, M;Thuret, I;Hongeng, S;Kulozik, A;Lal, A;Thrasher, A;Yannaki, E;Elliot, H;Tao, G;Asmal, M;Thompson, A;Lentiglobin Gene Therapy for Patients with Transfusion-Dependent β-Thalassemia (TDT): Results from the Phase 3 Northstar-2 and Northstar-3 StudiesBloodBackground Transfusion-dependent β-thalassemia (TDT) is a severe genetic disease caused by impaired β-globin production, leading to severe anemia, lifelong transfusion dependence with iron overload and serious comorbidities. Gene theraFull Paper
2018Li, C;Mathews, V;George, B;Kim, S;Hebert, K;Jiang, H;Li, C;Zhu, Y;Keesler, D;Agarwal, R;Boelens, J;Dvorak, C;Auletta, J;Goyal, R;Hanna, R;Kasow, K;Margolis, D;Shenoy, S;Walters, M;Eapen, M;Related and Unrelated Donor Transplantation for β Thalassemia Major: Results of an International SurveyBloodWe studied 1110 children, adolescents and young adults with β thalassemia major transplanted with grafts from HLA-matched relative (n=677; 61%), HLA-mismatched relative (n=78; 7%), HLA-matched unrelated (n=252; 23%) and HLA-mismatched unrelated (n=10Full Paper
2018Rasko, J;Thompson, A;Kwiatkowski, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;Schmidt, M;Kletzel, M;Leboulch, P;Vichinsky, E;Deary, B;Chen, Y;Asmal, M;Walters, M;Clinical Outcomes of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia Following Completion of the Northstar HGB-204 StudyBloodBackground Advances in red blood cell (RBC) transfusion and chelation have improved the prognosis of patients with transfusion-dependent β-thalassemia (TDT); however, many patients experience organ damage due to iron overload and otherFull Paper
2018Godwin, RL;Opatova, V;Garrison, NL;Hamilton, CA;Bond, JE;Phylogeny of a cosmopolitan family of morphologically conserved trapdoor spiders (Mygalomorphae, Ctenizidae) using Anchored Hybrid Enrichment, with a description of the family, Halonoproctidae Pocock 1901Mol. Phylogenet. Evol.29656103Phylogeny of a cosmopolitan family of morphologically conserved trapdoor spiders (Mygalomorphae, Ctenizidae) using Anchored Hybrid Enrichment, with a description of the family, Halonoproctidae Pocock 1901Full Paper
2018Hansen, R;Firdaus, SJ;Li, S;Janes, MR;Zhang, J;Liu, Y;Zarrinkar, PP;An Internally Controlled Quantitative Target Occupancy Assay for Covalent InhibitorsSci Rep30254226spectrometry. Clinical specimens. Clinical FFPE specimens were purchased from Conversant Bio (Huntsville, AL) and Asterand Bioscience (Detroit, MI). Conversant and Asterand comply with all applicable regulations. Animal studiesFull Paper
2018Potts, AH;Leng, Y;Babitzke, P;Romeo, T;Examination of Csr regulatory circuitry using epistasis analysis with RNA-seq (Epi-seq) confirms that CsrD affects gene expression via CsrA, CsrB and CsrCSci Rep29599472RNA-seq libraries were then generated with the Stranded RNA-Seq Library Preparation Kit for Illumina (KAPA) and NEBNext Multiplex Oligos for Illumina adaptors (NEB) according to the manufacturer’s instructions for 100 ng of starting material and a meFull Paper
2018Friedman, KM;Garrett, TE;Evans, JW;Horton, HM;Latimer, HJ;Seidel, SL;Horvath, CJ;Morgan, RA;Effective Targeting of Multiple B-Cell Maturation Antigen-Expressing Hematological Malignances by Anti-B-Cell Maturation Antigen Chimeric Antigen Receptor T CellsHum. Gene Ther.29641319LLC. Peripheral blood mononuclear cells (PBMCs) were isolated and cryopreserved in the authors' laboratory. Whole blood from two CLL patients was obtained from Conversant Biologics, Inc. Immunohistochemistry. TwentyFull Paper
2018Vasanthavel, S;Meenakshi, K;Nivedha, V;Ballamurugan, AM;Kannan, S;Tuning the structural and mechanical properties in ZrO2-SiO2 binary system through Y3+ inclusionsMater Sci Eng C Mater Biol Appl29519433Zirconia (ZrO 2 ) and Alumina (Al 2 O 3 ) are conversant bio-inert ceramics known for their superior mechanical properties while hydroxyapatite [Ca 5 (PO 4 ) 3 OH, HAP], β-tricalciumphosphate [β-Ca 3 (PO 4 ) 2 , β-TCP] and SiO 2 based bioactive glassFull Paper
2018Schlegel, C;Lapierre, LA;Weis, VG;Williams, JA;Kaji, I;Pinzon-Guzman, C;Prasad, N;Boone, B;Jones, A;Correa, H;Levy, SE;Han, X;Wang, M;Thomsen, K;Acra, S;Goldenring, JR;Reversible deficits in apical transporter trafficking associated with deficiency in diacylglycerol acyltransferaseTraffic30095213Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee.++Department of Surgery, Vanderbilt University School of Medicine, Nashville, Tennessee.++Department of Surgery, Vanderbilt University School of Medicine, NashvilleFull Paper
2018Selvan, N;George, S;Serajee, FJ;Shaw, M;Hobson, L;Kalscheuer, V;Prasad, N;Levy, SE;Taylor, J;Aftimos, S;Schwartz, CE;Huq, AM;Gecz, J;Wells, L;O-GlcNAc transferase missense mutations linked to X-linked intellectual disability deregulate genes involved in cell fate determination and signalingJ. Biol. Chem.29769320From the Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 30602.++From the Department of Biochemistry and Molecular Biology, Complex Carbohydrate Research Center, UniversitFull Paper
2018Edwardson, CF;Hollibaugh, JT;Composition and Activity of Microbial Communities along the Redox Gradient of an Alkaline, Hypersaline, LakeFront Microbiol29445359Samples were pooled and sequenced on one lane of an Illumina HiSeq2500 in Rapid Run mode with the 150PE (300 cycle) kit at HudsonAlpha Genomic Services Lab (Huntsville, AL). Bioinformatics of 16S rRNA SequencesFull Paper
2018Xu, J;Su, X;Bonizzoni, M;Zhong, D;Li, Y;Zhou, G;Nguyen, H;Tong, S;Yan, G;Chen, XG;Comparative transcriptome analysis and RNA interference reveal CYP6A8 and SNPs related to pyrethroid resistance in Aedes albopictusPLoS Negl Trop Dis30418967; RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). A total of 24 cDNA libraries were prepared individually from 12 susceptible and 12 resistant mosquFull Paper
2018Biase, FH;Kimble, KM;Functional signaling and gene regulatory networks between the oocyte and the surrounding cumulus cellsBMC Genomics29747587The samples were subjected to 16 cycles of PCR amplification. For all samples, we used 1 ng of amplified cDNA for library preparation with the Nextera XT DNA library preg Kit (Illumina, Inc.), as per the procedures described in the SMART-seq2 protocoFull Paper
2018Dickinson, SE;Griffin, BA;Elmore, MF;Kriese-Anderson, L;Elmore, JB;Dyce, PW;Rodning, SP;Biase, FH;Transcriptome profiles in peripheral white blood cells at the time of artificial insemination discriminate beef heifers with different fertility potentialBMC Genomics29426285Libraries were prepared with the TruSeq Stranded mRNA Library Prep kit (Illumina, Inc., San Diego, CA) following manufacturer’s instructions. Libraries were quantified with Qubit™ dsDNA High Sensitivity Assay Kit (Eurogene, OR) and quality was evaluaFull Paper
2018Lin, EM;Gong, J;Klempner, SJ;Chao, J;Advances in immuno-oncology biomarkers for gastroesophageal cancer: Programmed death ligand 1, microsatellite instability, and beyondWorld J. Gastroenterol.29991874Presence of tumor PD-L1 expression was a requirement for enrollment, with 40% of the patients screened demonstrating PD-L1-positive tumors. PD-L1 expression was detected using the 22C3 antibody with a prototype assay using QualTek or Dako platformsFull Paper
2018Fan, J;Lerner, J;Wyatt, MK;Cai, P;Peterson, K;Dong, L;Wistow, G;The klotho-related protein KLPH (lctl) has preferred expression in lens and is essential for expression of clic5 and normal lens suture formationExp. Eye Res.29425878XAR film at −70 °C. 2.3. In situ hybridization (ISH). Frozen tissues were cut into 10 μm sections, mounted on gelatin-coated slides and stored at −80 °C. ISH was performed at Phylogeny Inc. (Columbus, OH). Slides were fixed inFull Paper
2018Hiatt, SM;Amaral, MD;Bowling, KM;Finnila, CR;Thompson, ML;Gray, DE;Lawlor, JMJ;Cochran, JN;Bebin, EM;Brothers, KB;East, KM;Kelley, WV;Lamb, NE;Levy, SE;Lose, EJ;Neu, MB;Rich, CA;Simmons, S;Myers, RM;Barsh, GS;Cooper, GM;Systematic reanalysis of genomic data improves quality of variant interpretationClin. Genet.29652076HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama.Full Paper
2018Håvik, AL;Bruland, O;Aarhus, M;Kalland, KH;Stokowy, T;Lund-Johansen, M;Knappskog, PM;Screening for viral nucleic acids in vestibular schwannomaJ. Neurovirol.30168016; RNA sequencing on RNA extracted from five sVS tissue biopsies was performed by HudsonAlpha (HudsonAlpha Genomic Services Laboratory, 601 Genome Way, Huntsville, AL 35806 USA) using their "Poly A RNA-sequencing" service including 50 cycles of paireFull Paper
2018Mohan, S;Greenstein, I;Ng, C;Frazier, K;Nguyen, G;Harding, L;Barlow, D;Assessing N w-hydroxy-L-arginine applicability as a novel ethnic specific estrogen-negative breast cancer markerAmino Acids29260322Collection (Manassas, VA). We received de-identified plasma samples from Cooperative Human Tissue Network (Philadelphia, PA), Tissue-for-Research (Atlanta, GA), and Conversant Bio (Huntsville, AL). The de-identifiedFull Paper
2018Zhu, J;Gan, X;Ao, Q;Shen, X;Tan, Y;Chen, M;Luo, Y;Wang, H;Jiang, H;Li, C;Basal polarization of the immune responses to Streptococcus agalactiae susceptible and resistant tilapia (Oreochromis niloticus)Fish Shellfish Immunol.29454032; For each timepoint, equal amounts of RNA from the three replicates were pooled for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA)Full Paper
2018Porter, ET;Porter, FS;A Strain Gauge Monitor (SGM) for Continuous Valve Gape Measurements in Bivalve Molluscs in Response to Laboratory Induced Diel-cycling Hypoxia and pHJ Vis Exp30124658Name Company Catalog Number Comments Campbell CR 10x data logger Campbell Scientific, Logan, Utah Or other data logger. At Campbell the CR 10X has been replaced with the CR 1000 Campbell CR 10x multiplexer Campbell Scientific, Logan, Utah Data loggFull Paper
2018Yang, Y;Fu, Q;Liu, Y;Wang, X;Dunham, R;Liu, S;Bao, L;Zeng, Q;Zhou, T;Li, N;Qin, Z;Jiang, C;Gao, D;Liu, Z;Comparative transcriptome analysis reveals conserved branching morphogenesis related genes involved in chamber formation of catfish swimbladderPhysiol. Genomics29167198Equal amounts of RNA from the three replicates for each sample were pooled together for RNA-Seq library preparation. The remaining replicate pools were preserved as biological replicates for quantitative (q)RT-PCR validation. The cDNA libraries wereFull Paper
2018Cohen, RB;Delord, JP;Doi, T;Piha-Paul, SA;Liu, SV;Gilbert, J;Algazi, AP;Damian, S;Hong, RL;Le Tourneau, C;Day, D;Varga, A;Elez, E;Wallmark, J;Saraf, S;Thanigaimani, P;Cheng, J;Keam, B;Pembrolizumab for the Treatment of Advanced Salivary Gland Carcinoma: Findings of the Phase 1b KEYNOTE-028 StudyAm. J. Clin. Oncol.29462123Tumor PD-L1 positivity was determined at a central laboratory using formalin-fixed archived or newly obtained biopsy samples and a prototype immunohistochemical assay (QualTek Molecular Laboratories, Goleta, CA),17 based on the 22C3 antibody (Merck &Full Paper
2018Glover, ME;McCoy, CR;Shupe, EA;Unroe, KA;Jackson, NL;Clinton, SM;Perinatal exposure to the SSRI paroxetine alters the methylome landscape of the developing dentate gyrusEur. J. Neurosci.30585666; quantified (Quant-iT high sensitivity DNA Assay Kit, Invitrogen, Grand Island, NY). Next- generation sequencing was performed at HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) using NEBNext reagents (New EnglaFull Paper
2018Verwimp, C;Ruttink, T;Muylle, H;Van Glabeke, S;Cnops, G;Quataert, P;Honnay, O;Roldán-Ruiz, I;Temporal changes in genetic diversity and forage yield of perennial ryegrass in monoculture and in combination with red clover in swardsPLoS ONE30408053The protocol for the 112 tissue pool samples was slightly adjusted, based on the results of the validation step. In this case, the 50 μL bead-purified ligate was split into three aliquots, and 16.7 μL was used for three replicate PCR reactions to accFull Paper
2018Geister, KA;Timms, AE;Beier, DR;Optimizing Genomic Methods for Mapping and Identification of Candidate Variants in ENU Mutagenesis Screens Using Inbred MiceG3 (Bethesda)29208648... centrifugation. DNA was tested at SCRI for purity and quality using a Nanodrop spectrophotometer and the Qubit dsDNA Broad Range Assay (#Q32850; Thermo Fisher Scientific). DNA samples were shipped to HudsonAlpha Genomic Services Laboratory (HuntsFull Paper
2018Papanek, B;O'Dell, KB;Manga, P;Giannone, RJ;Klingeman, DM;Hettich, RL;Brown, SD;Guss, AM;Transcriptomic and proteomic changes from medium supplementation and strain evolution in high-yielding Clostridium thermocellum strainsJ. Ind. Microbiol. Biotechnol.30187243; Sequencing was completed using a SR50 sequencing protocol on an Illumina HiSeq 2500 platform (HudsonAlpha Genomic Services Laboratory; Huntsville, AL, USA). RNA-Seq analysis. The analysis of the raw RNA-Seq data was performed as previously describFull Paper
2018Ziai, J;Gilbert, HN;Foreman, O;Eastham-Anderson, J;Chu, F;Huseni, M;Kim, JM;CD8+ T cell infiltration in breast and colon cancer: A histologic and statistical analysisPLoS ONE29320521IV were represented. Colorectal cancer samples were procured from Conversant Bio and included moderately differentiated adenocarcinoma (9), mucinous carcinoma (1) and rectal (2) carcinoma cases. Samples containedFull Paper
2018Gallery, M;Zhang, J;Bradley, DP;Brauer, P;Cvet, D;Estevam, J;Danaee, H;Greenfield, E;Li, P;Manfredi, M;Loke, HK;Rabino, C;Stringer, B;Williamson, M;Wyant, T;Yang, J;Zhu, Q;Abu-Yousif, A;Veiby, OP;A monomethyl auristatin E-conjugated antibody to guanylyl cyclase C is cytotoxic to target-expressing cells in vitro and in vivoPLoS ONE29370189scanning system. Colon tissue microarrays (US Biomax) were stained and scored by two independent pathologists following a protocol developed at Qualtek Molecular Laboratories on their Tek-Mate automated stainer. Briefly ;ith hematoxylin and imagFull Paper
2018Lagatie, O;Verheyen, A;Nijs, E;Van Dorst, B;Batsa Debrah, L;Debrah, A;Supali, T;Sartono, E;Stuyver, LJ;Evaluation of the Diagnostic Performance of Onchocerca volvulus Linear Epitopes in a Peptide Enzyme-Linked Immunosorbent AssayAm. J. Trop. Med. Hyg.29313477(Glasgow, Scotland). A sec- ond healthy control sample set was composed of 49 plasma samples from healthy individuals from Belgium.20-24 Further- more, different sets of plasma samples were obtained from Discovery Life Sciences, IncFull Paper
2018Andreotta, PW;Arold, S;Kenyon, J;Spicer, D;Woodman, P;Berry, E;Brogan, T;Kong, S;Okerholm, P;Russell, V;Clarke, RW;Hava, DL;Inhaled calcium salts inhibit tobacco smoke-induced inflammation by modulating expression of chemokines and cytokinesPulm Pharmacol Ther30359782Secreted protein expression and gene expression were assayed 2 h after LPS stimulation. 2.5. Human macrophage cultures. Human CD14 + monocytes were purchased from Conversant Biologics and differentiated into macrophages in cultureFull Paper
2018Fall, L;Clevenger, J;McGregor, C;Assay development and marker validation for marker assisted selection of Fusarium oxysporum f. sp. niveum race 1 in watermelonMol Breedingfor sequencing. Library construction and whole genome Illumina sequencing on an Illumina HiSeq X (Illumina, Inc., San Diego, CA) were performed at HudsonAlpha Genomic services laboratory (Huntsville, AL). ConstructedFull Paper
2018Zobel-Thropp, PA;Bulger, EA;Cordes, MHJ;Binford, GJ;Gillespie, RG;Brewer, MS;Sexually dimorphic venom proteins in long-jawed orb-weaving spiders (Tetragnatha) comprise novel gene familiesPeerJ29876146RNA extractions from the BF population of T. versicolor males (two) and females (10) were shipped to the Genomic Services Lab at HudsonAlpha (Huntsville, AL, USA), where cDNA libraries were prepared from total RNA (poly-A isolation, Illumina TruSeq RFull Paper
2018Awad, H;Bratasz, A;Nuovo, G;Burry, R;Meng, X;Kelani, H;Brown, M;Ramadan, ME;Williams, J;Bouhliqah, L;Popovich, PG;Guan, Z;Mcallister, C;Corcoran, SE;Kaspar, B;Michele Basso, D;Otero, JJ;Kirsch, C;Davis, IC;Croce, CM;Michaille, JJ;Tili, E;MiR-155 deletion reduces ischemia-induced paralysis in an aortic aneurysm repair mouse model: Utility of immunohistochemistry and histopathology in understanding etiology of spinal cord paralysisAnn Diagn Pathol29966831The degree of permeability was determined by measuring the fluorescence of the receiver plate well solution. 2.5. MicroRNA in situ hybridization (ISH). ISH for miR-155 was performed by Phylogeny Inc. (Powell, OH), as previously described [16]Full Paper
2018Dufu, K;Patel, M;Oksenberg, D;Cabrales, P;GBT440 improves red blood cell deformability and reduces viscosity of sickle cell blood under deoxygenated conditionsClin. Hemorheol. Microcirc.29660913The study was approved by the IRB of the University of North Carolina (UNC) Sickle Cell program [IRB # 88-0343 (GCRC-101)]. Blood from trait individuals (AS) collected in sodium citrate vacutainers was purchased from Conversant Bio, Inc. (Huntsville,Full Paper
2018Smyth, E;Thuss-Patience, PC;Immune Checkpoint Inhibition in Gastro-Oesophageal CancerOncol Res Treat29705787gastro-oesophageal cancer. Unlike the studies with nivolumab, in the KEYNOTE- 12 patients were selected for PD-L1-positive status; however, a different antibody (QualTek 22C3) and scoring system were used. Eligibility wasFull Paper
2018Yuan, Z;Huang, W;Liu, S;Xu, P;Dunham, R;Liu, Z;Historical demography of common carp estimated from individuals collected from various parts of the world using the pairwise sequentially markovian coalescent approachGenetica29299799; Qiagen). Genome re-sequencing and sequence alignment. DNA library preparation and sequencing were conducted at HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA) following manufacturer's instructionsFull Paper
2018Vakulskas, CA;Dever, DP;Rettig, GR;Turk, R;Jacobi, AM;Collingwood, MA;Bode, NM;McNeill, MS;Yan, S;Camarena, J;Lee, CM;Park, SH;Wiebking, V;Bak, RO;Gomez-Ospina, N;Pavel-Dinu, M;Sun, W;Bao, G;Porteus, MH;Behlke, MA;A high-fidelity Cas9 mutant delivered as a ribonucleoprotein complex enables efficient gene editing in human hematopoietic stem and progenitor cellsNature medicine30082871ere used from either fresh cord blood (CB) (kindly provided by Binns Family program for Cord Blood Research), isolated from the peripheral blood of SCD patients, or frozen plerixafor mobilized CD34+ (AllCells). HSPCs were cultured at 250,000-500,000Full Paper
2018Good, Z;Sarno, J;Jager, A;Samusik, N;Aghaeepour, N;Simonds, EF;White, L;Lacayo, NJ;Fantl, WJ;Fazio, G;Gaipa, G;Biondi, A;Tibshirani, R;Bendall, SC;Nolan, GP;Davis, KL;Single-cell developmental classification of B cell precursor acute lymphoblastic leukemia at diagnosis reveals predictors of relapseNature medicine29505032Reagents and methods relevant to this work can also be found in the Life Sciences Reporting Summary. Fresh human bone marrow was obtained from healthy adult donors (n = 5; 3 females: 20, 27, 44 y.o., 2 males: 26, 28 y.o., average: 29 y.o.; AllCells,Full Paper
2018Deng, M;Gui, X;Kim, J;Xie, L;Chen, W;Li, Z;He, L;Chen, Y;Chen, H;Luo, W;Lu, Z;Xie, J;Churchill, H;Xu, Y;Zhou, Z;Wu, G;Yu, C;John, S;Hirayasu, K;Nguyen, N;Liu, X;Huang, F;Li, L;Deng, H;Tang, H;Sadek, AH;Zhang, L;Huang, T;Zou, Y;Chen, B;Zhu, H;Arase, H;Xia, N;Jiang, Y;Collins, R;You, MJ;Homsi, J;Unni, N;Lewis, C;Chen, GQ;Fu, YX;Liao, XC;An, Z;Zheng, J;Zhang, N;Zhang, CC;LILRB4 signalling in leukaemia cells mediates T cell suppression and tumour infiltrationNature30333625ockout was validated by flow cytometry. Leukemia cell and T cell co-culture assay In the co-culture assay, human T cells (5 × 104 per well) isolated from health donor peripheral blood (PB009-1-0, Allcells) were mixed with irradiated (28 Gy) indicateFull Paper
2018Roth, TL;Puig-Saus, C;Yu, R;Shifrut, E;Carnevale, J;Li, PJ;Hiatt, J;Saco, J;Krystofinski, P;Li, H;Tobin, V;Nguyen, DN;Lee, MR;Putnam, AL;Ferris, AL;Chen, JW;Schickel, JN;Pellerin, L;Carmody, D;Alkorta-Aranburu, G;Del Gaudio, D;Matsumoto, H;Morell, M;Mao, Y;Cho, M;Quadros, RM;Gurumurthy, CB;Smith, B;Haugwitz, M;Hughes, SH;Weissman, JS;Schumann, K;Esensten, JH;May, AP;Ashworth, A;Kupfer, GM;Greeley, SAW;Bacchetta, R;Meffre, E;Roncarolo, MG;Romberg, N;Herold, KC;Ribas, A;Leonetti, MD;Marson, A;Reprogramming human T cell function and specificity with non-viral genome targetingNature29995861Additional leukapheresis products from healthy donors were collected either under UCLA Institutional Review Board (IRB) approval #10-001598 or purchased from AllCells, LLC. All SaveFull Paper
2018Butler, A;Hoffman, P;Smibert, P;Papalexi, E;Satija, R;Integrating single-cell transcriptomic data across different conditions, technologies, and speciesNature biotechnology29608179We therefore repeated the original experiment, where PBMCs from a healthy human donor (ALLCELLS) were cultured with RPMI medium supplemented with 10% FBS and stimulated SaveFull Paper
2018Neal, JT;Li, X;Zhu, J;Giangarra, V;Grzeskowiak, CL;Ju, J;Liu, IH;Chiou, SH;Salahudeen, AA;Smith, AR;Deutsch, BC;Liao, L;Zemek, AJ;Zhao, F;Karlsson, K;Schultz, LM;Metzner, TJ;Nadauld, LD;Tseng, YY;Alkhairy, S;Oh, C;Keskula, P;Mendoza-Villanueva, D;De La Vega, FM;Kunz, PL;Liao, JC;Leppert, JT;Sunwoo, JB;Sabatti, C;Boehm, JS;Hahn, WC;Zheng, GXY;Davis, MM;Kuo, CJ;Organoid Modeling of the Tumor Immune MicroenvironmentCell30550791PBMCs, Pan T and CD19 + B cells used for the technical validation part of the immune profiling solution were obtained from AllCells (Catalog #: PB001, PB009-1F and PB010-0). Tumor SaveFull Paper
2018Buenrostro, JD;Corces, MR;Lareau, CA;Wu, B;Schep, AN;Aryee, MJ;Majeti, R;Chang, HY;Greenleaf, WJ;Integrated Single-Cell Analysis Maps the Continuous Regulatory Landscape of Human Hematopoietic DifferentiationCell29706549Healthy adult bone marrow allcells https://www.allcells.com/products/whole-bone-marrow- Samples were collected commercially from allcells. Further information about the donors SaveFull Paper
2018Su, R;Dong, L;Li, C;Nachtergaele, S;Wunderlich, M;Qing, Y;Deng, X;Wang, Y;Weng, X;Hu, C;Yu, M;Skibbe, J;Dai, Q;Zou, D;Wu, T;Yu, K;Weng, H;Huang, H;Ferchen, K;Qin, X;Zhang, B;Qi, J;Sasaki, AT;Plas, DR;Bradner, JE;Wei, M;Marcucci, G;Jiang, X;Mulloy, JC;Jin, J;He, C;Chen, J;R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA SignalingCell29249359R-2-hydroxyglutarate (R-2HG), produced at high levels by mutant isocitrate dehydrogenase 1/2 (IDH1/2) enzymes, was reported as an oncometabolite. We show here that R-2HG also SaveFull Paper
2018Choi, HW;Suwanpradid, J;Kim, IH;Staats, HF;Haniffa, M;MacLeod, AS;Abraham, SN;Perivascular dendritic cells elicit anaphylaxis by relaying allergens to mast cells via microvesiclesScience (New York, N.Y.)30409859100 U/ml penicillin, and 100 μg/ml streptomycin was utilized. In brief, purified peripheral blood CD34+ peripheral blood cells (Allcells) were suspended at 5×105 cells/ml in supplemented IMDM containing 100 ng/ml recombinant human (rh) SCF, 10 ng/mlFull Paper
2018Ting, PY;Parker, AE;Lee, JS;Trussell, C;Sharif, O;Luna, F;Federe, G;Barnes, SW;Walker, JR;Vance, J;Gao, MY;Klock, HE;Clarkson, S;Russ, C;Miraglia, LJ;Cooke, MP;Boitano, AE;McNamara, P;Lamb, J;Schmedt, C;Snead, JL;Guide Swap enables genome-scale pooled CRISPR-Cas9 screening in human primary cellsNature methods30297964AllCells and Lonza also obtained samples from outside sources with documented IRB compliance. Lot numbers can be found in the Nature Research Reporting Summary. SaveFull Paper
2018Jenkins, RW;Aref, AR;Lizotte, PH;Ivanova, E;Stinson, S;Zhou, CW;Bowden, M;Deng, J;Liu, H;Miao, D;He, MX;Walker, W;Zhang, G;Tian, T;Cheng, C;Wei, Z;Palakurthi, S;Bittinger, M;Vitzthum, H;Kim, JW;Merlino, A;Quinn, M;Venkataramani, C;Kaplan, JA;Portell, A;Gokhale, PC;Phillips, B;Smart, A;Rotem, A;Jones, RE;Keogh, L;Anguiano, M;Stapleton, L;Jia, Z;Barzily-Rokni, M;Cañadas, I;Thai, TC;Hammond, MR;Vlahos, R;Wang, ES;Zhang, H;Li, S;Hanna, GJ;Huang, W;Hoang, MP;Piris, A;Eliane, JP;Stemmer-Rachamimov, AO;Cameron, L;Su, MJ;Shah, P;Izar, B;Thakuria, M;LeBoeuf, NR;Rabinowits, G;Gunda, V;Parangi, S;Cleary, JM;Miller, BC;Kitajima, S;Thummalapalli, R;Miao, B;Barbie, TU;Sivathanu, V;Wong, J;Richards, WG;Bueno, R;Yoon, CH;Miret, J;Herlyn, M;Garraway, LA;Van Allen, EM;Freeman, GJ;Kirschmeier, PT;Lorch, JH;Ott, PA;Hodi, FS;Flaherty, KT;Kamm, RD;Boland, GM;Wong, KK;Dornan, D;Paweletz, CP;Barbie, DA;Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor SpheroidsCancer discovery29101162Freshly isolated human CD4+ and CD8+ T cells were obtained from AllCells. Cells were spun down and resuspended in serum free X-vivo15 media (Lonza Walkersville, Inc.) supplemented with 5 ng/mL IL17 and incubated overnight at 37°C. Cells were plated oFull Paper
2018Ryu, JK;Rafalski, VA;Meyer-Franke, A;Adams, RA;Poda, SB;Rios Coronado, PE;Pedersen, LØ;Menon, V;Baeten, KM;Sikorski, SL;Bedard, C;Hanspers, K;Bardehle, S;Mendiola, AS;Davalos, D;Machado, MR;Chan, JP;Plastira, I;Petersen, MA;Pfaff, SJ;Ang, KK;Hallenbeck, KK;Syme, C;Hakozaki, H;Ellisman, MH;Swanson, RA;Zamvil, SS;Arkin, MR;Zorn, SH;Pico, AR;Mucke, L;Freedman, SB;Stavenhagen, JB;Nelson, RB;Akassoglou, K;Fibrin-targeting immunotherapy protects against neuroinflammation and neurodegenerationNature immunology30323343wed by an incubation of 6.88 μM CD11b I-domain for 2 h at 25 ˚C. Cell Culture. Primary microglia and BMDMs were isolated, cultured, and treated with fibrin or LPS as previously described16. PBMCs (AllCells) (2×106 cells/mL) were plated in RPMI-1640Full Paper
2018Chorzalska, A;Morgan, J;Ahsan, N;Treaba, DO;Olszewski, AJ;Petersen, M;Kingston, N;Cheng, Y;Lombardo, K;Schorl, C;Yu, X;Zini, R;Pacilli, A;Tepper, A;Coburn, J;Hryniewicz-Jankowska, A;Zhao, TC;Oancea, E;Reagan, JL;Liang, O;Kotula, L;Quesenberry, PJ;Gruppuso, PA;Manfredini, R;Vannucchi, AM;Dubielecka, PM;Bone marrow-specific loss of ABI1 induces myeloproliferative neoplasm with features resembling human myelofibrosisBlood30213875CD34 + cells were isolated from the bone marrow of patients with PMF or from healthy marrow purchased from AllCells (Alameda, CA). Granulocytes were isolated from peripheral SaveFull Paper
2018Cairo, M;Parsons, S;Braniecki, S;Talano, J;Moore, T;Shi, Q;Keever-Taylor, C;Weinberg, R;Grossman, B;Dozor, A;Friedman, D;McKinstry, R;Verbsky, J;Moorthy, C;Weidner, R;Rodday, A;Ayello, J;Semidei-Pomales, M;Flower, A;Chu, Y;Morris, E;Mahanti, H;Fabricatore, S;Militano, O;Klejmont, L;van de Ven, C;Baxter-Lowe, L;Vichinsky, E;Walters, M;Shenoy, S;Significantly Improved Long Term Health Related Quality of Life (HRQL) and Neurocognition Following Familial Haploidentical Stem Cell Transplantation (HISCT) Utilizing CD34 Enrichment and Mononuclear (CD3) Addback in High Risk Patients with Sickle Cell Disease (SCD)BloodViaCord Processing Lab: Other: Medical Director; AllCells Inc.: Other: Medical Director. Shenoy: Novartis, Vertex, Bluebird Bio: Honoraria, Membership on an entity's Board of Directors or advisory committeesFull Paper
2018Jamieson, CHM;Millan, MT;Creasey, AA;Lomax, G;Donohoe, ME;Walters, MC;Abedi, M;Bota, DA;Zaia, JA;Adams, JS;CIRM Alpha Stem Cell Clinics: Collaboratively Addressing Regenerative Medicine ChallengesCell stem cell29859170Declaration of Interests All authors have CIRM funding to declare. M.C.W. is a Consultant for Bioverativ, Inc., Bluebird Bio, Inc., and Sangamo Biotherapeutics and a Medical Director for AllCells, Inc. and ViaCord Processing LabFull Paper
2018Cesana, M;Guo, MH;Cacchiarelli, D;Wahlster, L;Barragan, J;Doulatov, S;Vo, LT;Salvatori, B;Trapnell, C;Clement, K;Cahan, P;Tsanov, KM;Sousa, PM;Tazon-Vega, B;Bolondi, A;Giorgi, FM;Califano, A;Rinn, JL;Meissner, A;Hirschhorn, JN;Daley, GQ;A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout DevelopmentCell stem cell29625070Human CD34 + cells (Lonza and AllCells) from all sources were obtained as viable frozen states. The ages of the individuals used for the profiling and treatments are as follows: FL- SaveFull Paper
2018Kao, YR;Chen, J;Narayanagari, SR;Todorova, TI;Aivalioti, MM;Ferreira, M;Ramos, PM;Pallaud, C;Mantzaris, I;Shastri, A;Bussel, JB;Verma, A;Steidl, U;Will, B;Thrombopoietin receptor-independent stimulation of hematopoietic stem cells by eltrombopagScience translational medicine30209246Healthy cryopreserved human bone marrow cells were purchased from AllCells and ReachBio Research Labs; fresh unprocessed bone marrow cells were from Lonza. Samples of SaveFull Paper
2018Kou, X;Xu, X;Chen, C;Sanmillan, ML;Cai, T;Zhou, Y;Giraudo, C;Le, A;Shi, S;The Fas/Fap-1/Cav-1 complex regulates IL-1RA secretion in mesenchymal stem cells to accelerate wound healingScience translational medicine29540618Human bone marrow aspirates from healthy human adult volunteers (20 to 35 years of age) were purchased from AllCells LLC. Human GMSCs and SMSCs were isolated from the SaveFull Paper
2018Lai, D;Chen, M;Su, J;Liu, X;Rothe, K;Hu, K;Forrest, DL;Eaves, CJ;Morin, GB;Jiang, X;PP2A inhibition sensitizes cancer stem cells to ABL tyrosine kinase inhibitors in BCR-ABL+ human leukemiaScience translational medicine29437150Overcoming drug resistance and targeting leukemic stem cells (LSCs) remain major challenges in curing BCR-ABL + human leukemia. Using an advanced drug/proliferation screen, we SaveFull Paper
2018Ryan, DE;Taussig, D;Steinfeld, I;Phadnis, SM;Lunstad, BD;Singh, M;Vuong, X;Okochi, KD;McCaffrey, R;Olesiak, M;Roy, S;Yung, CW;Curry, B;Sampson, JR;Bruhn, L;Dellinger, DJ;Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAsNucleic acids research29216382Nucleofector kit (V4SP-3096). Cells were harvested at 48 hr post-transfection. Mobilized human peripheral blood CD34-positive hematopoietic stem and progenitor cells (CD34+ HSPCs) were purchased from AllCells (Alameda, CA) and thawed per manufacturerFull Paper
2018Syed, SA;Beurel, E;Loewenstein, DA;Lowell, JA;Craighead, WE;Dunlop, BW;Mayberg, HS;Dhabhar, F;Dietrich, WD;Keane, RW;de Rivero Vaccari, JP;Nemeroff, CB;Defective Inflammatory Pathways in Never-Treated Depressed Patients Are Associated with Poor Treatment ResponseNeuron30146307PBMCs from a non-hispanic white female healthy donor (age 50, weight: 142 lbs, height 68 in) unmedicated for a week, were freshly isolated at ALLCELLS. No other information SaveFull Paper
2018Lei, L;Chen, H;Xue, W;Yang, B;Hu, B;Wei, J;Wang, L;Cui, Y;Li, W;Wang, J;Yan, L;Shang, W;Gao, J;Sha, J;Zhuang, M;Huang, X;Shen, B;Yang, L;Chen, J;APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaksNature structural & molecular biology29323274Cryopreserved human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells, LLC (PB003F). CD8+ T cells were pre-enriched from the PBMCs with Easysep Human CD8+ T cell enrichment kit (19053, Stemcell Technologies) according to the manFull Paper
2018Stoeckius, M;Zheng, S;Houck-Loomis, B;Hao, S;Yeung, BZ;Mauck, WM;Smibert, P;Satija, R;Cell Hashing with barcoded antibodies enables multiplexing and doublet detection for single cell genomicsGenome biology30567574urther enable multiplexing strategies to generalize to diverse experiments regardless of species, tissue, or technology. Methods PBMC genotyping Peripheral blood mononuclear cells were obtained from AllCells (USA). Genomic DNA was purified using theFull Paper
2018Xu, Y;Zhao, W;Olson, SD;Prabhakara, KS;Zhou, X;Alternative splicing links histone modifications to stem cell fate decisionGenome biology30217220After reaching ~ 70% confluence, the adherent cells were harvested at the passage 1 (P1) hAT-MSC/AEY239. hBM-MSCs were isolated from commercially available fresh human bone marrow (hBM) aspirates (AllCells, Emeryville, CA, USA) and expanded followingFull Paper
2018Salas, LA;Koestler, DC;Butler, RA;Hansen, HM;Wiencke, JK;Kelsey, KT;Christensen, BC;An optimized library for reference-based deconvolution of whole-blood biospecimens assayed using the Illumina HumanMethylationEPIC BeadArrayGenome biology29843789urity cell subtypes (neutrophils (Neu), monocytes (Mono), B lymphocytes (Bcells), T helper lymphocytes (CD4T), T cytotoxic lymphocytes (CD8T), and natural killer lymphocytes (NK)) were purchased from AllCells corporation (Alameda, CA, USA) and STEMCFull Paper
2018Lim, SH;Kathuria, H;Tan, JJY;Kang, L;3D printed drug delivery and testing systems - a passing fad or the future?Advanced drug delivery reviews29778901Primary human dental pulp stem cells (DPSC) from a single donor (DPF003, AllCells, USA) were cultured in a complete growth media containing Dulbecco's Modified Eagle's Medium, supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. CFull Paper
2018Marabissi, D;Bartoli, G;Micciullo, L;Fantacci, R;Energy efficient multicast beamforming in ultra dense networks2018 IEEE Wireless Communications and Networking Conference (WCNC)This figure clearly shows the better behavior of our method in comparison with static approaches (ie, OnlyMacro, AllCells). Moreover, we can see that even if sub-optimum, our method is SaveFull Paper
2018Cao, L;Pauthner, M;Andrabi, R;Rantalainen, K;Berndsen, Z;Diedrich, JK;Menis, S;Sok, D;Bastidas, R;Park, SR;Delahunty, CM;He, L;Guenaga, J;Wyatt, RT;Schief, WR;Ward, AB;Yates, JR;Burton, DR;Paulson, JC;Differential processing of HIV envelope glycans on the virus and soluble recombinant trimerNature communications30209313Primary PBMC samples were obtained through the commercial vendor AllCells (Emeryville, CA The leukapak sample was obtained from a commercial vendor (AllCells) using a protocol SaveFull Paper
2018Mezger, A;Klemm, S;Mann, I;Brower, K;Mir, A;Bostick, M;Farmer, A;Fordyce, P;Linnarsson, S;Greenleaf, W;High-throughput chromatin accessibility profiling at single-cell resolutionNature communications30194434, and B cells were isolated from whole blood (AllCells, CA, USA) using EasySep Direct Human All human cells were obtained from AllCells with explicit consent to publish data for broad SaveFull Paper
2018Lyu, G;Guan, Y;Zhang, C;Zong, L;Sun, L;Huang, X;Huang, L;Zhang, L;Tian, XL;Zhou, Z;Tao, W;TGF-β signaling alters H4K20me3 status via miR-29 and contributes to cellular senescence and cardiac agingNature communications29967491The culture medium was MEM supplemented with 10% FBS (GIBCO), and the culture dish was gelatin-coated before inoculation of primary human umbilical vascular endothelial cells (HUVECs) purchased from ALLCELLS Co. Ltd., Shanghai, China. Cells were testFull Paper
2018Xu, L;Gordon, R;Farmer, R;Pattanayak, A;Binkowski, A;Huang, X;Avram, M;Krishna, S;Voll, E;Pavese, J;Chavez, J;Bruce, J;Mazar, A;Nibbs, A;Anderson, W;Li, L;Jovanovic, B;Pruell, S;Valsecchi, M;Francia, G;Betori, R;Scheidt, K;Bergan, R;Precision therapeutic targeting of human cancer cell motilityNature communications29934502od cells, hemoglobin, and platelets. Hematopoietic stem cell colony formation assay Fourteen-day colony formation assays were conducted as described by us39, using human cord blood CD34+ stem cells (AllCells Inc.), using MethoCultExpress colony groFull Paper
2018Yang, R;Yu, T;Kou, X;Gao, X;Chen, C;Liu, D;Zhou, Y;Shi, S;Tet1 and Tet2 maintain mesenchymal stem cell homeostasis via demethylation of the P2rX7 promoterNature communications29858571(PFA, Sigma-Aldrich) after 16 days of culturing. Clusters with more than 50 cells were counted as colonies under microscopy. Isolation and culture of human BMMSCs We purchased human bone marrow from AllCells LLC (Alameda, CA, USA), which were aspiraFull Paper
2018Lummertz da Rocha, E;Rowe, RG;Lundin, V;Malleshaiah, M;Jha, DK;Rambo, CR;Li, H;North, TE;Collins, JJ;Daley, GQ;Reconstruction of complex single-cell trajectories using CellRouterNature communications29497036es) would act as perturbations that could be explored by CLR to identify putative regulatory relationships. In vitro erythroid differentiation Mobilized peripheral blood CD34+ stem/progenitor cells (AllCells) were obtained from three independent donFull Paper
2018Ravi, R;Noonan, KA;Pham, V;Bedi, R;Zhavoronkov, A;Ozerov, IV;Makarev, E;V Artemov, A;Wysocki, PT;Mehra, R;Nimmagadda, S;Marchionni, L;Sidransky, D;Borrello, IM;Izumchenko, E;Bedi, A;Bifunctional immune checkpoint-targeted antibody-ligand traps that simultaneously disable TGFβ enhance the efficacy of cancer immunotherapyNature communications29467463)(4-8-week old) were irradiated at 200 cGy, followed by adoptive transfer of human BM CD34 + cells (7 × 10 4 per mouse) from a normal donor (HLA-matched to the tumor) (ALLCELLS) SaveFull Paper
2018Bak, RO;Dever, DP;Porteus, MH;CRISPR/Cas9 genome editing in human hematopoietic stem cellsNature protocols29370156Fresh or frozen CD34 + human HSPCs (either acquired per institutional protocol or purchased from various commercial sources, including AllCells (CB-derived, cat. no. CB008F; mPB- SaveFull Paper
2018Adine, C;Ng, KK;Rungarunlert, S;Souza, GR;Ferreira, JN;Engineering innervated secretory epithelial organoids by magnetic three-dimensional bioprinting for stimulating epithelial growth in salivary glandsBiomaterials30025245Human dental pulp stem cells (hDPSCs) were commercially obtained from AllCells (Alameda, CA, USA) from three different donors. These stem cells were derived from the pulp tissues of exfoliated deciduous teeth, primary incisors, or permanent third molFull Paper
2018Jiang, L;Zhang, W;Wei, L;Zhou, Q;Yang, G;Qian, N;Tang, Y;Gao, Y;Jiang, X;Early effects of parathyroid hormone on vascularized bone regeneration and implant osseointegration in aged ratsBiomaterials29960821Human umbilical vein endothelial cells (HUVECs) (Allcells, Shanghai, China) were cultured and their in vitro recruitment and angiogenesis abilities were measured. PTH (1-34) was added at a concentration of 10 nM in the lower chambers of 24-well transFull Paper
2018Odegaard, JI;Vincent, JJ;Mortimer, S;Vowles, JV;Ulrich, BC;Banks, KC;Fairclough, SR;Zill, OA;Sikora, M;Mokhtari, R;Abdueva, D;Nagy, RJ;Lee, CE;Kiedrowski, LA;Paweletz, CP;Eltoukhy, H;Lanman, RB;Chudova, DI;Talasaz, A;Validation of a Plasma-Based Comprehensive Cancer Genotyping Assay Utilizing Orthogonal Tissue- and Plasma-Based MethodologiesClinical cancer research : an official journal of the American Association for Cancer Research29691297Contrived analytic samples were generated using similarly prepared cfDNA from healthy donors (AllCells, Inc.) and cfDNA isolated as above from the culture supernatant of model cell SaveFull Paper
2018L'Italien, L;Orozco, O;Abrams, T;Cantagallo, L;Connor, A;Desai, J;Ebersbach, H;Gelderblom, H;Hoffmaster, K;Lees, E;Maacke, H;Schleyer, S;Skegro, D;Lee-Hoeflich, ST;Mechanistic Insights of an Immunological Adverse Event Induced by an Anti-KIT Antibody Drug Conjugate and Mitigation StrategiesClinical cancer research : an official journal of the American Association for Cancer Research29615457Human peripheral blood cell-derived mast cells (PBC-MCs) were generated from mobilized peripheral blood CD34 + cells isolated from healthy donors (AllCells #mPB017F). Mast cells SaveFull Paper
2018Cromer, MK;Vaidyanathan, S;Ryan, DE;Curry, B;Lucas, AB;Camarena, J;Kaushik, M;Hay, SR;Martin, RM;Steinfeld, I;Bak, RO;Dever, DP;Hendel, A;Bruhn, L;Porteus, MH;Global Transcriptional Response to CRISPR/Cas9-AAV6-Based Genome Editing in CD34+ Hematopoietic Stem and Progenitor CellsMolecular therapy : the journal of the American Society of Gene Therapy30005866CD34+ HSPCs used for gene expression microarrays were obtained as frozen stock derived from mobilized peripheral blood thawed according to manufacturer’s instructions (AllCells). For follow-up experiments, CD34+ HSPCs were obtained fresh from cord blFull Paper
2018Ligtenberg, MA;Pico de Coaña, Y;Shmushkovich, T;Yoshimoto, Y;Truxova, I;Yang, Y;Betancur-Boissel, M;Eliseev, AV;Wolfson, AD;Kiessling, R;Self-Delivering RNAi Targeting PD-1 Improves Tumor-Specific T Cell Functionality for Adoptive Cell Therapy of Malignant MelanomaMolecular therapy : the journal of the American Society of Gene Therapy29735366ation (Ficoll-Paque Plus; GE Healthcare). T cells were purified using the Pan T cell isolation kit (Miltenyi Biotec) following manufacturer’s instructions. Peripheral blood CD3+ Pan T cells from AllCells (PB009-1F) were used for sdRNA validation andFull Paper
2018Georgiadis, C;Preece, R;Nickolay, L;Etuk, A;Petrova, A;Ladon, D;Danyi, A;Humphryes-Kirilov, N;Ajetunmobi, A;Kim, D;Kim, JS;Qasim, W;Long Terminal Repeat CRISPR-CAR-Coupled "Universal" T Cells Mediate Potent Anti-leukemic EffectsMolecular therapy : the journal of the American Society of Gene Therapy29605708CliniMACS Prodigy using tubing set TS520 (130-019-002, Miltenyi Biotec) and used cryopreserved leukapheresis harvests (Allcells, Alameda, USA) cultured in TexMACS good manufacturing practice (GMP) medium (170-076-307, Miltenyi Biotec) supplemented wiFull Paper
2018Heffner, GC;Bonner, M;Christiansen, L;Pierciey, FJ;Campbell, D;Smurnyy, Y;Zhang, W;Hamel, A;Shaw, S;Lewis, G;Goss, KA;Garijo, O;Torbett, BE;Horton, H;Finer, MH;Gregory, PD;Veres, G;Prostaglandin E2 Increases Lentiviral Vector Transduction Efficiency of Adult Human Hematopoietic Stem and Progenitor CellsMolecular therapy : the journal of the American Society of Gene Therapy29102562CD34-enriched mPB samples from healthy human donors were obtained from AllCells (Emeryville, CA, USA), Key Biologics (Memphis, TN, USA), and primary patients under Save;Methods Cell Culture Research-grade GFP-, globin-, and ALDP-containing LVVs werFull Paper
2018Mokhtari, S;Colletti, E;Yin, W;Sanada, C;Lamar, Z;Simmons, PJ;Walker, S;Bishop, C;Atala, A;Zanjani, ED;Porada, CD;Almeida-Porada, G;A human bone marrow mesodermal-derived cell population with hemogenic potentialLeukemia29467489al bone tissues were obtained from Advanced Bioscience Resources (Alameda, CA). Human adult bone marrow (BM) was obtained from healthy adult donors after informed consent, or from commercial sources (AllCells, LLC, Alameda, CA). Fetal and adult tissuFull Paper
2018Tan, YT;Ye, L;Xie, F;Beyer, AI;Muench, MO;Wang, J;Chen, Z;Liu, H;Chen, SJ;Kan, YW;Respecifying human iPSC-derived blood cells into highly engraftable hematopoietic stem and progenitor cells with a single factorProceedings of the National Academy of Sciences of the United States of America29386396Frozen human PB mobilized CD34+ cells and PB mononuclear cells were purchased from AllCells (www.allcells.com). The protocols involving human iPSCs were approved by Human Research Committee and Human Gamete, Embryo and Stem Cells Research Committee (Full Paper
2018Li, Y;Carpenito, C;Wang, G;Surguladze, D;Forest, A;Malabunga, M;Murphy, M;Zhang, Y;Sonyi, A;Chin, D;Burtrum, D;Inigo, I;Pennello, A;Shen, L;Malherbe, L;Chen, X;Hall, G;Haidar, JN;Ludwig, DL;Novosiadly, RD;Kalos, M;Discovery and preclinical characterization of the antagonist anti-PD-L1 monoclonal antibody LY3300054Journal for immunotherapy of cancer29712568s analyzed using GraphPad Prism software [25]. Mixed leukocyte reaction (MLR) CD14+ monocytes were isolated from frozen human peripheral blood mononuclear cells (PBMC) obtained from a healthy donor (AllCells, Alameda, CA) with Human Monocyte IsolatiFull Paper
2018Liu, Y;Jing, H;Kou, X;Chen, C;Liu, D;Jin, Y;Lu, L;Shi, S;PD-1 is required to maintain stem cell properties in human dental pulp stem cellsCell death and differentiation29472716Isolation and culture of human BMMSCs Human bone marrow aspirates from healthy human adult volunteers (20-35 years of age) were purchased from AllCells LLC. hBMMSCs were cultured as reported previously [40]Full Paper
2018Willingham, SB;Ho, PY;Hotson, A;Hill, C;Piccione, EC;Hsieh, J;Liu, L;Buggy, JJ;McCaffery, I;Miller, RA;A2AR Antagonism with CPI-444 Induces Antitumor Responses and Augments Efficacy to Anti-PD-(L)1 and Anti-CTLA-4 in Preclinical ModelsCancer immunology research30131376PBMCs were purchased from AllCells and either used fresh or cryopreserved for future use. Fresh PBMCs were cultured at a density of 10 7 cells/mL, and cryopreserved cells were SaveFull Paper
2018Siriwon, N;Kim, YJ;Siegler, E;Chen, X;Rohrs, JA;Liu, Y;Wang, P;CAR-T Cells Surface-Engineered with Drug-Encapsulated Nanoparticles Can Ameliorate Intratumoral T-cell HypofunctionCancer immunology research29720380Frozen human peripheral blood mononuclear cells (PBMC) were obtained from AllCells. Thawed PBMCs were cultured in T-cell medium (TCM) containing X-VIVO 15 serum-free SaveFull Paper
2018Oeller, M;Laner-Plamberger, S;Hochmann, S;Ketterl, N;Feichtner, M;Brachtl, G;Hochreiter, A;Scharler, C;Bieler, L;Romanelli, P;Couillard-Despres, S;Russe, E;Schallmoser, K;Strunk, D;Selection of Tissue Factor-Deficient Cell Transplants as a Novel Strategy for Improving Hemocompatibility of Human Bone Marrow Stromal CellsTheranostics29507631Three additional BM samples (10 mL each) were purchased via CellSystems from ALLCELLS (USA). The mean age of the male BM donors was 34 years (range 20-46) and of the SaveFull Paper
2018de la Puente, P;Luderer, MJ;Federico, C;Jin, A;Gilson, RC;Egbulefu, C;Alhallak, K;Shah, S;Muz, B;Sun, J;King, J;Kohnen, D;Salama, NN;Achilefu, S;Vij, R;Azab, AK;Enhancing proteasome-inhibitory activity and specificity of bortezomib by CD38 targeted nanoparticles in multiple myelomaJournal of controlled release : official journal of the Controlled Release Society29196043Bone Marrow mononuclear cells (BM MNCs) from 5 different patient samples were purchased from Allcells (Alameda, CA). Peripheral blood mononuclear cells (PB MNCs) were SaveFull Paper
2018Chen, Y;Mao, P;Snijders, AM;Wang, D;Senescence chips for ultrahigh-throughput isolation and removal of senescent cellsAging cell29336105Fresh human whole blood from healthy donors collected within 24 hr was purchased from AllCells Inc. (Alameda, CA). Human MSCs were purchased from Lonza (Lonza, Swiss). SaveFull Paper
2018Charlesworth, CT;Camarena, J;Cromer, MK;Vaidyanathan, S;Bak, RO;Carte, JM;Potter, J;Dever, DP;Porteus, MH;Priming Human Repopulating Hematopoietic Stem and Progenitor Cells for Cas9/sgRNA Gene TargetingMolecular therapy. Nucleic acids30195800CD34 + HSPCs derived from mobilized peripheral blood were purchased from AllCells, and CD34 + HSPCs derived from cord blood were provided from donors under informed consent Save;ollowing the manufacturer’s instructions. Titers were measured after bFull Paper
2018Vaidyanathan, S;Azizian, KT;Haque, AKMA;Henderson, JM;Hendel, A;Shore, S;Antony, JS;Hogrefe, RI;Kormann, MSD;Porteus, MH;McCaffrey, AP;Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC PurificationMolecular therapy. Nucleic acids30195789CD34 + HSPCs derived from mobilized peripheral blood donated by male donors were purchased from AllCells (Alemeda, CA). Cells were thawed according to the manufacturer's Save;tection System luminometer (Promega, E8032) with a 10-s integration per wFull Paper
2018Yoshinaga, T;Seki, T;Miki, S;Miyamoto, T;Suyama-Kagitani, A;Kawauchi-Miki, S;Kobayashi, M;Sato, A;Stewart, E;Underwood, M;Fujiwara, T;Novel secondary mutations C56S and G149A confer resistance to HIV-1 integrase strand transfer inhibitorsAntiviral research29410019kanamycin sulfate. Peripheral blood mononuclear cells (PBMCs; AllCells, Alameda, CA) were cultured in RPMI 1640 medium supplemented with 10% FBS, 60 μg/mL of kanamycin sulfate, and 10 μg/mL of phytohemagglutinin (PHA)-M (Roche, Indianapolis, IN) forFull Paper
2018Li, S;Hong, Z;Wang, Z;Li, F;Mei, J;Huang, L;Lou, X;Zhao, S;Song, L;Chen, W;Wang, Q;Liu, H;Cai, Y;Yu, H;Xu, H;Zeng, G;Wang, Q;Zhu, J;Liu, X;Tan, N;Wang, C;The Cyclopeptide Astin C Specifically Inhibits the Innate Immune CDN Sensor STINGCell reports30566866hPBMCs and IMR-90 (female) cells were purchased from AllCells and Cobioer Bioscience, respectively. The gender information of hPBMCs is not available from the supplier. The SaveFull Paper
2018Osorio, FG;Rosendahl Huber, A;Oka, R;Verheul, M;Patel, SH;Hasaart, K;de la Fonteijne, L;Varela, I;Camargo, FD;van Boxtel, R;Somatic Mutations Reveal Lineage Relationships and Age-Related Mutagenesis in Human HematopoiesisCell reports30485801nd Cat# 302005; RRID:AB_314205 CD11c-FITC BioLegend Cat# 301603; RRID:AB_314173 ------------------------- Biological Samples ------------------------- Whole bone marrow samples A,C,D,E AllCells ABM001 Whole bone marrow sample B BFull Paper
2018Zhu, YP;Padgett, L;Dinh, HQ;Marcovecchio, P;Blatchley, A;Wu, R;Ehinger, E;Kim, C;Mikulski, Z;Seumois, G;Madrigal, A;Vijayanand, P;Hedrick, CC;Identification of an Early Unipotent Neutrophil Progenitor with Pro-tumoral Activity in Mouse and Human Bone MarrowCell reports30157427Fresh BM samples of anonymous healthy adult donors were obtained from AllCells, Inc. (Alameda, CA). The cells were stained for either flow cytometry or FACS-sorting following protocols described in the Flow Cytometry and Cell Sorting sectionFull Paper
2018Schaer, DA;Beckmann, RP;Dempsey, JA;Huber, L;Forest, A;Amaladas, N;Li, Y;Wang, YC;Rasmussen, ER;Chin, D;Capen, A;Carpenito, C;Staschke, KA;Chung, LA;Litchfield, LM;Merzoug, FF;Gong, X;Iversen, PW;Buchanan, S;de Dios, A;Novosiadly, RD;Kalos, M;The CDK4/6 Inhibitor Abemaciclib Induces a T Cell Inflamed Tumor Microenvironment and Enhances the Efficacy of PD-L1 Checkpoint BlockadeCell reports29539425Breast cancer cell lines MCF-7 and MDA-MB-468 (American Type Culture Collection [ATCC]) were treated with DMSO and abemaciclib (500 nM) for 8 days. RNA was isolated as described above. Jurkat T cells (ATCC) or primary human T cells (AllCells) were stFull Paper
2018Shi, J;Zhang, M;Zhang, L;Deng, H;Epigallocatechin-3-gallate attenuates microcystin-LR-induced apoptosis in human umbilical vein endothelial cells through activation of the NRF2/HO-1 pathwayEnvironmental pollution (Barking, Essex : 1987)29679944HUVECs were obtained from Allcells Technology (Shanghai, China) and seeded in complete medium (HUVEC-004, AllCells Technology) in a humidified atmosphere of 5% CO 2 at 37 SaveFull Paper
2018Wienert, B;Shin, J;Zelin, E;Pestal, K;Corn, JE;In vitro-transcribed guide RNAs trigger an innate immune response via the RIG-I pathwayPLoS biology30011268HSPCs from mobilized peripheral blood (Allcells) were thawed and cultured in StemSpan SFEM medium (StemCell Technologies) supplemented with StemSpan CC110 cocktail ( SaveFull Paper
2018Yang, W;Sun, Z;Hua, C;Wang, Q;Xu, W;Deng, Q;Pan, Y;Lu, L;Jiang, S;Chidamide, a histone deacetylase inhibitor-based anticancer drug, effectively reactivates latent HIV-1 provirusMicrobes and infection29126877J-Lat A2 cells [25] (obtained from NIH AIDS Reagent Program), J-Lat C11 cells [26], PBMCs (Allcells), ACH-2 cells [27] and U1 cells [28] were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS) and 1% Pen/strep in a 37 °C incubator containFull Paper
2018Ito, R;Maruoka, S;Soda, K;Katano, I;Kawai, K;Yagoto, M;Hanazawa, A;Takahashi, T;Ogura, T;Goto, M;Takahashi, R;Toyoshima, S;Okayama, Y;Izuhara, K;Gon, Y;Hashimoto, S;Ito, M;Nunomura, S;A humanized mouse model to study asthmatic airway inflammation via the human IL-33/IL-13 axisJCI insight30385714Commercially available human cord blood-derived CD34 + cells (AllCells LLC or Lonza) were used in this study. The cells were prepared according to the manufacturer's instructions. SaveFull Paper
2018Antebi, B;Rodriguez, LA;Walker, KP;Asher, AM;Kamucheka, RM;Alvarado, L;Mohammadipoor, A;Cancio, LC;Short-term physiological hypoxia potentiates the therapeutic function of mesenchymal stem cellsStem cell research & therapy30305185arrow MSCs (pMSCs) were isolated from mononuclear cells (MNCs) obtained from bone marrow aspirates, as previously described [39]. Human bone marrow MSCs (hMSCs) were isolated from MNCs purchased from AllCells LLC (Emeryville, CA, USA). MNCs were cultFull Paper
2018Senyuk, V;Patel, P;Mahmud, N;Rondelli, D;Blockade of TNFα to Improve Human CD34+ Cell Repopulating Activity in Allogeneic Stem Cell TransplantationFrontiers in immunology30723481Healthy donor G-CSF mobilized peripheral blood stem cells (PBSC) from AllCells (Alameda, CA) and PB cells from healthy volunteers were utilized in this study. Mononuclear cells ( SaveFull Paper
2018Cui, C;Su, M;Lin, Y;Lai, L;A CD300c-Fc Fusion Protein Inhibits T Cell ImmunityFrontiers in immunology30498497peripheral blood CD3 + Pan T Cells that were negatively isolated from mononuclear cells using an indirect immunomagnetic Pan-T labeling system were purchased from ALLCELLS, SaveFull Paper
2018Cobbold, SP;Adams, E;Howie, D;Waldmann, H;CD4+ T Cell Fate Decisions Are Stochastic, Precede Cell Division, Depend on GITR Co-Stimulation, and Are Associated With Uropodium DevelopmentFrontiers in immunology29967616t CV (%) is indicated. In all experiments, P values ofFull Paper
2018Burton, OT;Epp, A;Fanny, ME;Miller, SJ;Stranks, AJ;Teague, JE;Clark, RA;van de Rijn, M;Oettgen, HC;Tissue-Specific Expression of the Low-Affinity IgG Receptor, FcγRIIb, on Human Mast CellsFrontiers in immunology29928276genic for membrane-bound human stem cell factor (SCF) [NOD.Cg-PrkdcscidIl2rgtm1Wjl Tg(PGK1-KITLG*220)441Daw/SzJ] were engrafted with 5 × 104 CD34+ hematopoietic stem cells (HSC) from cord blood (AllCells) for 16-24 weeks. Wild-type BALB/c, C57BL/6J,Full Paper
2018White, CH;Beliakova-Bethell, N;Lada, SM;Breen, MS;Hurst, TP;Spina, CA;Richman, DD;Frater, J;Magiorkinis, G;Woelk, CH;Transcriptional Modulation of Human Endogenous Retroviruses in Primary CD4+ T Cells Following Vorinostat TreatmentFrontiers in immunology29706951rials and Methods Isolation of Primary CD4+ T Cells For subsequent total RNA-Seq analysis, cryopreserved primary CD4+ T cells that were viably frozen were obtained from four different healthy donors (AllCells, Inc., Emeryville, CA, USA) and thawed inFull Paper
2018Bhaskaran, N;Liu, Z;Saravanamuthu, SS;Yan, C;Hu, Y;Dong, L;Zelenka, P;Zheng, L;Bletsos, V;Harris, R;Harrington, B;Weinberg, A;Thiele, CJ;Ye, F;Pandiyan, P;Identification of Casz1 as a Regulatory Protein Controlling T Helper Cell Differentiation, Inflammation, and ImmunityFrontiers in immunology29467767done at NIAID, NIH under an approved protocol, and in compliance with the NIAID Institutional Animal Care and Use Committee’s guidelines. Human cells were obtained from commercially available PBMC (AllCells). Reagents and Antibodies Purified or fluoFull Paper
2018Hanazawa, A;Ito, R;Katano, I;Kawai, K;Goto, M;Suemizu, H;Kawakami, Y;Ito, M;Takahashi, T;Generation of Human Immunosuppressive Myeloid Cell Populations in Human Interleukin-6 Transgenic NOG MiceFrontiers in immunology29456539ined in the Central Institute for Experimental Animals (CIEA) under specific pathogen-free conditions. Transplantation of Human HSCs Human umbilical-cord-blood-derived CD34+ HSCs were purchased from Allcells (Alameda, CA, USA). For transplantation,Full Paper
2018Seto, AG;Beatty, X;Lynch, JM;Hermreck, M;Tetzlaff, M;Duvic, M;Jackson, AL;Cobomarsen, an oligonucleotide inhibitor of miR-155, co-ordinately regulates multiple survival pathways to reduce cellular proliferation and survival in cutaneous T-cell lymphomaBritish journal of haematology30125933miR‐155, a microRNA associated with poor prognosis in lymphoma and leukaemia, has been implicated in the progression of mycosis fungoides ( MF ), the most common form of SaveFull Paper
2018Makar, T;Nimmagadda, VK;Guda, PR;Hampton, B;Huang, W;Kane, MA;Fishman, PS;Pessac, B;Bever, CT;Trisler, D;A subset of mobilized human hematopoietic stem cells express germ layer lineage genes which can be modulated by culture conditionsStem cell research & therapy29720239Human buffy coat cells and CD34 + cells from peripheral blood were purchased from ALLCELLS (Alameda, CA, USA). Human buffy coat cells were obtained by differential SaveFull Paper
2018Fujisawa, K;Hara, K;Takami, T;Okada, S;Matsumoto, T;Yamamoto, N;Sakaida, I;Evaluation of the effects of ascorbic acid on metabolism of human mesenchymal stem cellsStem cell research & therapy29625581MSCs were derived from bone marrow cells (AllCells, Alameda, CA,USA) and were used at passage (P)4. Mononuclear cells (MNCs) were purchased from Lonza, Tokyo, Japan. Flow SaveFull Paper
2018Thekkeparambil Chandrabose, S;Sriram, S;Subramanian, S;Cheng, S;Ong, WK;Rozen, S;Kasim, NHA;Sugii, S;Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogrammingStem cell research & therapy29559008The list of commercial (Lonza and Allcells) and patient-derived dental cells used in this manuscript can be found in Additional file 1: Table S1. DPSCs were grown in vitro in Poietics SaveFull Paper
2018Johnson, HWB;Lowe, E;Anderl, JL;Fan, A;Muchamuel, T;Bowers, S;Moebius, DC;Kirk, C;McMinn, DL;Required Immunoproteasome Subunit Inhibition Profile for Anti-Inflammatory Efficacy and Clinical Candidate KZR-616 ((2 S,3 R)- N-(( S)-3-(Cyclopent-1-en-1-yl)-1-(( R)-2-methyloxiran-2-yl)-1-oxopropan-2-yl)-3-hydroxy-3-(4-methoxyphenyl)-2-(( S)-2-(2-morpholinoacetamido)propanamido)propenamide)Journal of medicinal chemistry30380863Cryopreserved peripheral blood mononuclear cells (PBMCs) from normal healthy volunteers were purchased from AllCells (Alameda, CA) and cultured in RPMI 1640 media (with 300 mg/L l-glutamine) supplemented with 5% heat-inactivated FBS, 100 IU penicilliFull Paper
2018Vazquez, ML;Kaila, N;Strohbach, JW;Trzupek, JD;Brown, MF;Flanagan, ME;Mitton-Fry, MJ;Johnson, TA;TenBrink, RE;Arnold, EP;Basak, A;Heasley, SE;Kwon, S;Langille, J;Parikh, MD;Griffin, SH;Casavant, JM;Duclos, BA;Fenwick, AE;Harris, TM;Han, S;Caspers, N;Dowty, ME;Yang, X;Banker, ME;Hegen, M;Symanowicz, PT;Li, L;Wang, L;Lin, TH;Jussif, J;Clark, JD;Telliez, JB;Robinson, RP;Unwalla, R;Identification of N-{cis-3-[Methyl(7H-pyrrolo[2,3-d]pyrimidin-4-yl)amino]cyclobutyl}propane-1-sulfonamide (PF-04965842): A Selective JAK1 Clinical Candidate for the Treatment of Autoimmune DiseasesJournal of medicinal chemistry29298069Cryopreserved human bone marrow CD34+ cells (cat. no. ABM022F) were purchased from Allcells. Frozen bone marrow CD34+ cells were thawed, washed once with IMEM, resuspended in Stem Span medium (cat. no. 09600; STEMCELL Technologies, Vancouver, Canada)Full Paper
2018Xu, H;Liao, C;Zuo, P;Liu, Z;Ye, BC;Magnetic-Based Microfluidic Device for On-Chip Isolation and Detection of Tumor-Derived ExosomesAnalytical chemistry30234974CD63 antibody (anti-CD63) from Biolegend (USA), biotin-labeled mouse antihuman CD9 antibody (anti-CD9) from Allcells Biotech Co., Ltd. (Shanghai, China), and HRP-conjugated antimouse IgG from QF Biosciences Co., Ltd. (Shanghai, China). MagCapture ExoFull Paper
2018Xu, H;Liao, C;Liu, Y;Ye, BC;Liu, B;Iron Phthalocyanine Decorated Nitrogen-Doped Graphene Biosensing Platform for Real-Time Detection of Nitric Oxide Released from Living CellsAnalytical chemistry29508999Human umbilical vein endothelial cells (HUVECs) (ALLCELLS, Shanghai) were cultured in a humidified incubator (95% air with 5% CO2) at 37 °C with a complete medium designed for optimal growth of endothelial cells in vitro. A 6 mm thick PDMS containingFull Paper
2018Yasinska, IM;Gonçalves Silva, I;Sakhnevych, SS;Ruegg, L;Hussain, R;Siligardi, G;Fiedler, W;Wellbrock, J;Bardelli, M;Varani, L;Raap, U;Berger, S;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV;High mobility group box 1 (HMGB1) acts as an "alarmin" to promote acute myeloid leukaemia progressionOncoimmunology29872582lemented with 10% foetal bovine serum, penicillin (50 IU/ml) and streptomycin sulphate (50 µg/ml). Primary human AML mononuclear blasts (AML-PB001 F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordancFull Paper
2018Lam, J;Bellayr, IH;Marklein, RA;Bauer, SR;Puri, RK;Sung, KE;Functional Profiling of Chondrogenically Induced Multipotent Stromal Cell Aggregates Reveals Transcriptomic and Emergent Morphological Phenotypes Predictive of Differentiation CapacityStem cells translational medicine30084545rived MSCs were obtained from eight different donors purchased from RoosterBio (RB9, RB12, RB14, RB16; Frederick, MD) at passage 1 (P1), Lonza (8F560, 167696; Walkersville, MD) at passage 2 (P2), and AllCells (PCBM1632, PCBM1641; Alameda, CA) at P2 (Full Paper
2018George, MJ;Prabhakara, K;Toledano-Furman, NE;Wang, YW;Gill, BS;Wade, CE;Olson, SD;Cox, CS;Clinical Cellular Therapeutics Accelerate Clot FormationStem cells translational medicine30070065Bone marrow derived MSCs (BM MSCs) were isolated from commercially available fresh human bone marrow aspirates (AllCells, Emeryville, CA) or obtained from fresh bone marrow SaveFull Paper
2018Marcellino, BK;Hoffman, R;Tripodi, J;Lu, M;Kosiorek, H;Mascarenhas, J;Rampal, RK;Dueck, A;Najfeld, V;Advanced forms of MPNs are accompanied by chromosomal abnormalities that lead to dysregulation of TP53Blood advances30563882Normal bone marrow MNCs were obtained from AllCells (Alameda, CA). RNA was extracted from MNCs, using RNeasy mini kit (Fisher Scientific, Waltham, MA), and was reverse SaveFull Paper
2018Wei, Y;Zheng, H;Bao, N;Jiang, S;Bueso-Ramos, CE;Khoury, J;Class, C;Lu, Y;Lin, K;Yang, H;Ganan-Gomez, I;Starczynowski, DT;Do, KA;Colla, S;Garcia-Manero, G;KDM6B overexpression activates innate immune signaling and impairs hematopoiesis in miceBlood advances30275007BM samples from healthy individuals were obtained from AllCells (Emeryville, CA). CD34 + cells were isolated using MicroBead Kit (Miltenyi Biotec). For colony formation, BM CD34 + SaveFull Paper
2018Witt, RG;Kreger, EM;Buckman, LB;Moradi, PW;Ho, PT;Derderian, SC;Tsai, P;Baker, C;Schramm, N;Cleary, R;Garcia, JV;MacKenzie, TC;Systemic multilineage engraftment in mice after in utero transplantation with human hematopoietic stem cellsBlood advances29344586Human cord blood CD34 + cells were either purchased from AllCells (Alameda, CA) or purified from cord blood collected at time of delivery from normal-term infants. CD34 + cells were SaveFull Paper
2018Murray, C;Miwa, H;Dhar, M;Park, DE;Pao, E;Martinez, J;Kaanumale, S;Loghin, E;Graf, J;Raddassi, K;Kwok, WW;Hafler, D;Puleo, C;Di Carlo, D;Unsupervised capture and profiling of rare immune cells using multi-directional magnetic ratchetingLab on a chip30039125Cryopreserved Peripheral Blood Mononuclear Cells (PBMCs) were purchased from ALLCELLs (Alameda, CA, USA; Cat No. PB003F) and stored at −180°C in vapor phase liquid nitrogen until ready for experimental use. Cells were thawed by placing cryovial in 37Full Paper
2018Romero-Masters, JC;Ohashi, M;Djavadian, R;Eichelberg, MR;Hayes, M;Bristol, JA;Ma, S;Ranheim, EA;Gumperz, J;Johannsen, EC;Kenney, SC;An EBNA3C-deleted Epstein-Barr virus (EBV) mutant causes B-cell lymphomas with delayed onset in a cord blood-humanized mouse modelPLoS pathogens30125329Commercially available CD34-depleted human cord blood mononuclear cells (AllCells) were infected with 5000 Green Raji Units of wild-type or mutant EBV strains in vitro for 1 ½ hours SaveFull Paper
2018Miller, AZ;Satchie, A;Tannenbaum, AP;Nihal, A;Thomson, JA;Vereide, DT;Expandable Arterial Endothelial Precursors from Human CD34+ Cells Differ in Their Proclivity to Undergo an Endothelial-to-Mesenchymal TransitionStem cell reports29320761scribed in Figure 5 as a means to transiently label transfected cells. Sources of Primary Cells Frozen primary CD34+ cells purified from umbilical cord blood or adult bone marrow were obtained from AllCells (for cord blood, products CB005F and CB009Full Paper
2018Tsai, HC;Velichko, S;Lee, S;Wu, R;Cholera toxin enhances interleukin-17A production in both CD4+ and CD8+ cells via a cAMP/protein kinase A-mediated interleukin-17A promoter activationImmunology29377102Peripheral blood mononuclear cells were purchased from Allcells, LLC (Alameda, CA) or isolated from the leukopak filters from the Delta Blood Bank in Stockton, CA. Total CD4 + T SaveFull Paper
2018Yang, R;Liu, Y;Yu, T;Liu, D;Shi, S;Zhou, Y;Zhou, Y;Hydrogen sulfide maintains dental pulp stem cell function via TRPV1-mediated calcium influxCell death discovery3006205000 μg/ml streptomycin and passaged, as previously reported27. Human bone marrow MSCs BMMSC culture Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells (Emeryville, CA, USA). Human bone marrow progeFull Paper
2018Zhou, Z;Shao, T;Qin, M;Miao, X;Chang, Y;Sheng, W;Wu, F;Yu, Y;The effects of autophagy on vascular endothelial cells induced by airborne PM2.5Journal of environmental sciences (China)29628085In this study, human umbilical vein endothelial cells (HUVECs) were obtained from Allcells, China. The cells were routinely maintained in an HUVEC complete culture medium (Allcells, SaveFull Paper
2018Figueroa, I;Leipold, D;Leong, S;Zheng, B;Triguero-Carrasco, M;Fourie-O'Donohue, A;Kozak, KR;Xu, K;Schutten, M;Wang, H;Polson, AG;Kamath, AV;Prediction of non-linear pharmacokinetics in humans of an antibody-drug conjugate (ADC) when evaluation of higher doses in animals is limited by tolerability: Case study with an anti-CD33 ADCmAbs2975769861 Samples included normal human whole blood donors (n = 6 from Genentech, Inc. South San Francisco, CA; for phenotyping lymphocyte and myeloid subsets), normal human bone marrow donors (n = 5 from ALLCells, Alameda, CA; for phenotyping hematopoieticFull Paper
2018Jiang, N;Wang, Y;Yin, Y;Wei, R;Ying, G;Li, B;Qiu, T;van Rijn, P;Tian, G;Yan, Q;Dai, H;Busscher, H;Li, S;Yetisen, A;Yang, X;bFGF and Poly-RGD Cooperatively Establish Biointerface for Stem Cell Adhesion, Proliferation, and DifferentiationAdvanced Materials InterfaceshBMMSCs Culture: Freshly isolated human bone marrow mononuclear cells obtained from a 23-year-old donor were purchased from ALLCELLS (Emeryville, CA, US), and cultured with α-minimum essential media (MEM)/15% FBS/glutamine (2 mmol L−1)/penicillin (10Full Paper
2018Hoff, FW;Hu, CW;Qiu, Y;Ligeralde, A;Yoo, SY;Mahmud, H;de Bont, ESJM;Qutub, AA;Horton, TM;Kornblau, SM;Recognition of Recurrent Protein Expression Patterns in Pediatric Acute Myeloid Leukemia Identified New Therapeutic TargetsMolecular cancer research : MCR29669821patients (57 pre-B ALL and 16 T-ALL, patient demographics are presented in Supplemental Table S2), 10 cryopreserved normal BM CD34+ (AllCells, Almeda CA), and 127 leukemic cell line samples. The methods and validation of the technique, as well as theFull Paper
2018Bates, AM;Fischer, CL;Abhyankar, VP;Johnson, GK;Guthmiller, JM;Progulske-Fox, A;Brogden, KA;Matrix Metalloproteinase Response of Dendritic Cell, Gingival Epithelial Keratinocyte, and T-Cell Transwell Co-Cultures Treated with Porphyromonas gingivalis Hemagglutinin-BInternational journal of molecular sciences30544510ls, GE keratinocytes, and T-cells were chosen for this experiment due to their presence in the periodontium and their innate immune functions. Human monocyte-derived immature myeloid dendritic cells (AllCells, Alameda, CA, USA) were thawed, washed wiFull Paper
2018Chen, Q;Smith, S;Fong, T;Shirazian, A;Lee, J;Chapman, L;Tong, J;Vaz, W;Practical considerations for sourcing clinical-grade human tissue to support development and production of emerging commercial cellular therapiesCytotherapyQ. Chen, S. Smith, T. Fong, A. Shirazian, J. Lee, L. Chapman, J. Tong, W. Vaz Commercial, AllCells, LLC, Alameda, California, United States The rapid clinical advancement and commercial manufacturing of cellular therapeutics pose numerous industrialFull Paper
2018Okragly, AJ;Morin, SM;DeRosa, D;Martin, AP;Johnson, KW;Johnson, MP;Benschop, RJ;Human mast cells release the migraine-inducing factor pituitary adenylate cyclase-activating polypeptide (PACAP)Cephalalgia : an international journal of headache29103295Human umbilical cord blood-derived mast cells were produced by in vitro differentiation of positively selected cord blood CD34+ progenitor cells (Allcells) as previously described (37,38). Cells were cultured in Serum Free Expansion Media (SFEM Cat.Full Paper
2018Oberst, MD;Augé, C;Morris, C;Kentner, S;Mulgrew, K;McGlinchey, K;Hair, J;Hanabuchi, S;Du, Q;Damschroder, M;Feng, H;Eck, S;Buss, N;de Haan, L;Pierce, AJ;Park, H;Sylwester, A;Axthelm, MK;Picker, L;Morris, NP;Weinberg, A;Hammond, SA;Potent Immune Modulation by MEDI6383, an Engineered Human OX40 Ligand IgG4P Fc Fusion ProteinMolecular cancer therapeutics29545330Ligation of OX40 (CD134, TNFRSF4) on activated T cells by its natural ligand (OX40L, CD252, TNFSF4) enhances cellular survival, proliferation, and effector functions such as cytokine SaveFull Paper
2018Fujisawa, K;Takami, T;Okada, S;Hara, K;Matsumoto, T;Yamamoto, N;Yamasaki, T;Sakaida, I;Analysis of Metabolomic Changes in Mesenchymal Stem Cells on Treatment with Desferrioxamine as a Hypoxia Mimetic Compared with Hypoxic ConditionsStem cells (Dayton, Ohio)29577517Cells and Cell Culturing BMSCs were obtained from bone marrow cells (age: 34, male) purchased from AllCells (Alameda, CA), and BMSCs were proliferated using passage 4 and passage 16. BMSCs were confirmed to be CD45 (-), CD11b (-), CD90 (+), CD73 (+),Full Paper
2018Fujii, S;Miura, Y;Fujishiro, A;Shindo, T;Shimazu, Y;Hirai, H;Tahara, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T;Graft-Versus-Host Disease Amelioration by Human Bone Marrow Mesenchymal Stromal/Stem Cell-Derived Extracellular Vesicles Is Associated with Peripheral Preservation of Naive T Cell PopulationsStem cells (Dayton, Ohio)29239062Human MSCs were isolated from BM samples purchased from AllCells (Alameda, CA) using our previously published method [21, 22]. BM samples were obtained from three different SaveFull Paper
2018Harting, MT;Srivastava, AK;Zhaorigetu, S;Bair, H;Prabhakara, KS;Toledano Furman, NE;Vykoukal, JV;Ruppert, KA;Cox, CS;Olson, SD;Inflammation-Stimulated Mesenchymal Stromal Cell-Derived Extracellular Vesicles Attenuate InflammationStem cells (Dayton, Ohio)29076623Human mesenchymal stromal cells (hMSCs) were isolated from commercially available fresh human bone marrow aspirates of a 34-year-old female (AllCells, Alameda, CA) using SaveFull Paper
2018Meader, E;Barta, T;Melguizo-Sanchis, D;Tilgner, K;Montaner, D;El-Harouni, AA;Armstrong, L;Lako, M;Pluripotent Stem Cell-Derived Hematopoietic Progenitors Are Unable to Downregulate Key Epithelial-Mesenchymal Transition-Associated miRNAsStem cells (Dayton, Ohio)29047185and TaqMan MicroRNA Assays were used for the qRT‐PCR (all from Thermo Fisher Scientific) RNU44 and RNU48 were chosen as internal controls. CD34+ human adult bone marrow and CD34+ cord blood cells (AllCells, Alameda, CA, USA) were used as positive conFull Paper
2018Wang, J;Li, T;Feng, J;Li, L;Wang, R;Cheng, H;Yuan, Y;Kaempferol protects against gamma radiation-induced mortality and damage via inhibiting oxidative stress and modulating apoptotic molecules in vivo and vitroEnvironmental toxicology and pharmacology29705372To investigate the potential protective effect of kaempferol, a representative flavonoid, against radiation induced mortality and injury in vivo and vitro.C57BL/6 male mice and human SaveFull Paper
2018Garbuzova-Davis, S;Haller, E;Navarro, S;Besong, TE;Boccio, KJ;Hailu, S;Khatib, M;Sanberg, PR;Appel, SH;Borlongan, CV;Transplantation of human bone marrow stem cells into symptomatic ALS mice enhances structural and functional blood-spinal cord barrier repairExperimental neurology30172620Accumulating evidence shows alterations in the blood-brain barrier (BBB) and blood-spinal cord barrier (BSCB) in ALS patients and in animal models of disease, mainly by endothelial SaveFull Paper
2018Huang, L;Zhang, X;Ma, X;Zhang, D;Li, D;Feng, J;Pan, X;Lü, J;Wang, X;Liu, X;Berberine alleviates endothelial glycocalyx degradation and promotes glycocalyx restoration in LPS-induced ARDSInternational immunopharmacology30308440(Shanghai, China) and grown to confluence in 25 cm 2 flasks supplemented with complete culture medium, which was provided by AllCells. The cells were incubated under 95% SaveFull Paper
2018Romero, Z;DeWitt, M;Walters, MC;Promise of gene therapy to treat sickle cell diseaseExpert opinion on biological therapy30324810MC Walters is the Medical Director of AllCells, Inc and ViaCord Processing Lab; Consultant for Sangamo Therapeutics, INc, bluebird bio, Inc., and Bioverativ, Inc. The authors have no other relevant affiliations or financial involvement with any organFull Paper
2018Hu, Y;Yao, X;Liu, Q;Wang, Y;Liu, R;Cui, S;Ding, J;Left-Right Symmetry or Asymmetry of Cells on Stripe-Like Micropatterned Material SurfacesChinese Journal of ChemistryrMSCs were bought from Allcells Biological Technology, Shanghai. hMSCs were from Cyagen Biosciences, Suzhou. HFFs and HUVECs were products of Bogoo Biological Technology, Shanghai. The cells of rMSCs were incubated in minimum essential medium α (MEMFull Paper
2018Yuan, X;Han, L;Fu, P;Zeng, H;Lv, C;Chang, W;Runyon, RS;Ishii, M;Han, L;Liu, K;Fan, T;Zhang, W;Liu, R;Cinnamaldehyde accelerates wound healing by promoting angiogenesis via up-regulation of PI3K and MAPK signaling pathwaysLaboratory investigation; a journal of technical methods and pathology29463877Cell culture and animal maintenance Primary HUVECs were purchased from AllCells (Emeryville, CA, USA). With the exception of the homocysteine-rescue tube assay, each batch of HUVECs was examined using flow cytometry and was shown to be 99% CD31 positFull Paper
2018Huang, T;Mathieu, M;Lee, S;Wang, X;Kee, YS;Bevers, JJ;Ciferri, C;Estavez, A;Wong, M;Chiang, NY;Nakamura, G;Brezski, RJ;Molecular characterization of human anti-hinge antibodies derived from single-cell cloning of normal human B cellsThe Journal of biological chemistry29191832PBMCs were isolated from a Leuko Pak (AllCells) using Ficoll-Paque gradient fractionation (GE Healthcare). PBMCs were then frozen in 90% fetal bovine serum (FBS) supplemented with 10% dimethyl sulfoxide (DMSO) and stored in liquid nitrogen until thawFull Paper
2018Smith, AJ;Graves, B;Child, R;Rice, PJ;Ma, Z;Lowman, DW;Ensley, HE;Ryter, KT;Evans, JT;Williams, DL;Immunoregulatory Activity of the Natural Product Laminarin Varies Widely as a Result of Its Physical PropertiesJournal of immunology (Baltimore, Md. : 1950)29246954PBMCs obtained via leukapheresis were purchased from AllCells (Alameda, CA) and cryopreserved in 50% RPMI 1640 with 40% FBS/10% DMSO, after extensive washing in 1× PBS. SaveFull Paper
2018Yang, C;Huan, Z;Wang, X;Wu, C;Chang, J;3D Printed Fe Scaffolds with HA Nanocoating for Bone RegenerationACS biomaterials science & engineering33418749initial density of 1× 104 rabbit bone marrow mesenchymal stem cells (rBMSCs) (Allcells, Silicon Valley, CA, USA) in 48-well tissue culture plates and then incubated at 37 °C wiht 5% CO2. After 24 h, the cell culture medium was changed for the first tFull Paper
2018Piao, XM;Gao, F;Zhu, JX;Wang, LJ;Zhao, X;Li, X;Sheng, MM;Zhang, Y;Cucurbitacin B inhibits tumor angiogenesis by triggering the mitochondrial signaling pathway in endothelial cellsInternational journal of molecular medicine29717773HUVECs and HepG2 cells were obtained from Allcells, LLC (Shanghai, China). CuB (purity 99%) was purchased from Chengdu Herbpurify Co., Ltd. (Chengdu, China). DMEM/F-12, fetal bovine serum (FBS), was from Gibco (Carlsbad, CA, USA). EGM-2 complete mediFull Paper
2018Zhu, H;Yu, B;Yang, L;Exogenous and Endogenous Stem Cells for Skeletal RegenerationStem cells international30581472HUVECs were purchased from AllCells, Ltd. (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth factors [19]. To investigate the SaveFull Paper
2018Zhang, M;Yu, W;Niibe, K;Zhang, W;Egusa, H;Tang, T;Jiang, X;The Effects of Platelet-Derived Growth Factor-BB on Bone Marrow Stromal Cell-Mediated Vascularized Bone RegenerationStem cells international30515221HUVECs were purchased from AllCells, Ltd. (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth factors [19]. To investigate the SaveFull Paper
2018Kwak, J;Choi, SJ;Oh, W;Yang, YS;Jeon, HB;Jeon, ES;Cobalt Chloride Enhances the Anti-Inflammatory Potency of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells through the ERK-HIF-1α-MicroRNA-146a-Mediated Signaling PathwayStem cells international30254683103 cells/well) were seeded and maintained at 37°C in a humidified incubator for 2-4 hr and then cocultured with responder peripheral blood mononuclear cells (PBMCs; 1 × 105 cells/well; AllCells, Boston, MA, USA) and stimulator PBMCs (1 × 105 cells/Full Paper
2018Chen, B;Ni, Y;Liu, J;Zhang, Y;Yan, F;Bone Marrow-Derived Mesenchymal Stem Cells Exert Diverse Effects on Different Macrophage SubsetsStem cells international30140291y directed activation. Western blot was used to detect the expression of iNOS and Arg-1 in the cells to further verify the M1 and M2 polarization. 2.5. Preparation of MSC-CM BMMSC was purchased from AllCells, LLC (Emeryville, CA), and MSCs at passagFull Paper
2018Jiang, J;Xu, K;Wang, L;Xin, W;Zhao, G;Huang, M;Li, S;Luan, X;Fang, J;Pharmacology study of a chimeric decoy receptor trap fusion protein on retina neovascularization by dual blockage of VEGF and FGF-2European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences29715501Human Umbilical Vein Endothelial Cells (HUVECs) were purchased from AllCells (Shanghai, China) and maintained in a complete HUVEC medium (Cat# H-004, AllCells)Full Paper
2018Yen, J;Fiorino, M;Liu, Y;Paula, S;Clarkson, S;Quinn, L;Tschantz, WR;Klock, H;Guo, N;Russ, C;Yu, VWC;Mickanin, C;Stevenson, SC;Lee, C;Yang, Y;TRIAMF: A New Method for Delivery of Cas9 Ribonucleoprotein Complex to Human Hematopoietic Stem CellsScientific reports30389991Cryopreserved bone marrow derived CD34 + HSPCs were purchased from Lonza or AllCells and thawed according to vendors' instructions. Cells were cultured at 37 C 5% CO 2 in SaveFull Paper
2018Han, SS;Shim, HE;Park, SJ;Kim, BC;Lee, DE;Chung, HM;Moon, SH;Kang, SW;Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for TrackingScientific reports30181604The protocol was approved by the Committee on Biological Research of Korea Institute of Toxicology and Institutional Review Board (P01-201509-41-001). hUCB-EPCs culture hUCB-EPCs were purchased from AllCells (Alameda, CA, USA). hUCB-EPCs culture werFull Paper
2018Burgenson, D;Gurramkonda, C;Pilli, M;Ge, X;Andar, A;Kostov, Y;Tolosa, L;Rao, G;Rapid recombinant protein expression in cell-free extracts from human bloodScientific reports29934577anced the expression of Nluc, but RNase inhibitor did not have a significant effect (Fig. 2C,D).Figure 1Production of human leukocyte extract and Nluc expression. (A) Leukopak was purchased form the AllCells, Inc. (B) The cells were clarified by centFull Paper
2018Valton, J;Guyot, V;Boldajipour, B;Sommer, C;Pertel, T;Juillerat, A;Duclert, A;Sasu, BJ;Duchateau, P;Poirot, L;A Versatile Safeguard for Chimeric Antigen Receptor T-Cell ImmunotherapiesScientific reports29895885Primary T-cells isolated from PBMC (ALLCELLS) were activated and transfected according to previously described methods 13 . Four days after their activation by Dynabeads human T SaveFull Paper
2018Gaudin, A;Tolar, M;Peters, OA;Lipoxin A4 Attenuates the Inflammatory Response in Stem Cells of the Apical Papilla via ALX/FPR2Scientific reports29892010eated with mitomycin C (Sigma-Aldrich) at the final concentration of 25 μg/mL for 30 min, and then washed 4 times with PBS + 10% Human Serum. Human peripheral blood mononuclear cells (PBMC) (AllCells, Alameda, CA, USA) in dilution ratios 1:1, 1:5 andFull Paper
2018Lim, SH;Sun, Y;Thiruvallur Madanagopal, T;Rosa, V;Kang, L;Enhanced Skin Permeation of Anti-wrinkle Peptides via Molecular ModificationScientific reports29371611215 nm. A calibration curve was conducted using the respective standard solutions from 1 ppm to 1000 ppm. DPSC Cell Culture Primary human dental pulp stem cells (DPSC) from a single donor (DPF003, AllCells, USA) were cultured in a complete growth meFull Paper
2018Ruppert, KA;Nguyen, TT;Prabhakara, KS;Toledano Furman, NE;Srivastava, AK;Harting, MT;Cox, CS;Olson, SD;Human Mesenchymal Stromal Cell-Derived Extracellular Vesicles Modify Microglial Response and Improve Clinical Outcomes in Experimental Spinal Cord InjuryScientific reports29323194hMSCs were isolated from commercially available fresh human bone marrow aspirates of a 34 year old male (AllCells, Alameda, CA) using density centrifugation and plastic adherence SaveFull Paper
2018Li, C;Sunderic, K;Nicoll, SB;Wang, S;Downregulation of Heat Shock Protein 70 Impairs Osteogenic and Chondrogenic Differentiation in Human Mesenchymal Stem CellsScientific reports29323151Human MSCs were isolated from fresh human bone marrow purchased from AllCells (Alameda, CA) according to manufacturer's instructions as previously described 2 and cultured SaveFull Paper
2018Bloom, DD;Reshetylo, S;Nytes, C;Goodsett, CT;Hematti, P;Blockade of BAFF Receptor BR3 on T Cells Enhances Their Activation and CytotoxicityJournal of immunotherapy (Hagerstown, Md. : 1997)29309290Primary human T cells were obtained from leukopheresis products purchased from AllCells, LLC (Alameda, CA) or Key Biologics, LLC (Memphis, TN). Upon arrival, PBLs were isolated SaveFull Paper
2018Mair, F;Prlic, M;OMIP-044: 28-color immunophenotyping of the human dendritic cell compartmentCytometry. Part A : the journal of the International Society for Analytical Cytology29356334Cryopreserved PBMCs were obtained from AllCells, stained and subsequently aquired on a BD FACSymphony. Optimal PMT voltages were determined by voltage titration experiments and iterative refinement. All reagents were titrated prior to use. For FigureFull Paper
2018Doering, CB;Denning, G;Shields, JE;Fine, EJ;Parker, ET;Srivastava, A;Lollar, P;Spencer, HT;Preclinical Development of a Hematopoietic Stem and Progenitor Cell Bioengineered Factor VIII Lentiviral Vector Gene Therapy for Hemophilia AHuman gene therapy30160169Genetically modified, autologous hematopoietic stem and progenitor cells (HSPCs) represent a new class of genetic medicine. Following this therapeutic paradigm, we are developing a SaveFull Paper
2018Liu, XY;Xu, HX;Li, JK;Zhang, D;Ma, XH;Huang, LN;Lü, JH;Wang, XZ;Neferine Protects Endothelial Glycocalyx via Mitochondrial ROS in Lipopolysaccharide-Induced Acute Respiratory Distress SyndromeFrontiers in physiology29520236Human umbilical vein endothelial cells (HUVECs) were grown to confluence and cultured in 25 cm 2 flasks supplemented with complete culture medium (AllCells, Shanghai, China) in a SaveFull Paper
2018Zeng, Y;Dabay, M;George, V;Seetharaman, S;de Arruda Indig, M;Graminske, S;Kimpel, N;Schmidt, A;Boerner, A;Paradiso, S;Delman, T;Li, Y;Litvak, V;Oreizy, F;Chen, A;Saleminik, M;Mosqueda, F;Lin, A;Judge, K;Comparison of Flow Cytometric Methods for the Enumeration of Residual Leucocytes in Leucoreduced Blood Products: A Multicenter StudyCytometry. Part A : the journal of the International Society for Analytical Cytology29345745Per enrollment needs, leucoreduced PLTs and RBCs with autologous white blood cell sources were also purchased from qualified vendors that produce apheresis PLTs and RBCs through standard procedures (AllCells, LLC, Alameda, CA; Stanford Blood Center,Full Paper
2018Song, B;Chen, Y;Liu, Y;Wan, C;Zhang, L;Zhang, W;NPAS2 regulates proliferation of acute myeloid leukemia cells via CDC25A-mediated cell cycle progression and apoptosisJournal of cellular biochemistry30536616NHC cell specimens were separated from normal cells in bone marrow bought from AllCells, LLC (Emeryville, CA). Cells were preserved in 5% CO 2 at 37C in RPMI-1640 media SaveFull Paper
2018Lewis, G;Christiansen, L;McKenzie, J;Luo, M;Pasackow, E;Smurnyy, Y;Harrington, S;Gregory, P;Veres, G;Negre, O;Bonner, M;Staurosporine Increases Lentiviral Vector Transduction Efficiency of Human Hematopoietic Stem and Progenitor CellsMolecular therapy. Methods & clinical development30038935CD34-enriched mPB samples from healthy human donors were obtained from AllCells (Emeryville, CA) and Key Biologics (Memphis, TN). Research-grade staurosporine was obtained Save;apeutics. Materials and Methods Cell Culture Research-grade BlaM-contaFull Paper
2018Mendoza, M;Ballesteros, A;Qiu, Q;Pow Sang, L;Shashikumar, S;Casares, S;Brumeanu, TD;Generation and testing anti-influenza human monoclonal antibodies in a new humanized mouse model (DRAGA: HLA-A2. HLA-DR4. Rag1 KO. IL-2Rγc KO. NOD)Human vaccines & immunotherapeutics29135340Briefly, HLA-DRB1*0401 + umbilical cord bloods (UCB) purchased from AllCells and Promocell were enriched for CD34 + stem cells to more than 60% when using EasySep, Human SaveFull Paper
2018Low, EK;Brudvik, E;Kuhlman, B;Wilson, PF;Almeida-Porada, G;Porada, CD;Microgravity Impairs DNA Damage Repair in Human Hematopoietic Stem/Progenitor Cells and Inhibits Their Differentiation into Dendritic CellsStem cells and development29901426Healthy human BM mononuclear cells (BMMNC) were purchased from AllCells, LLC (Alameda, CA). CD34+ HSC were isolated from BMMNC using anti-human CD34 MicroBeads and the MiniMACS magnetic-based positive-selection system (Miltenyi Biotec, Inc., Auburn,Full Paper
2018Almeida-Porada, G;Rodman, C;Kuhlman, B;Brudvik, E;Moon, J;George, S;Guida, P;Sajuthi, SP;Langefeld, CD;Walker, SJ;Wilson, PF;Porada, CD;Exposure of the Bone Marrow Microenvironment to Simulated Solar and Galactic Cosmic Radiation Induces Biological Bystander Effects on Human HematopoiesisStem cells and development29698131In brief, upon return from BNL, MSC from each irradiation group were overlaid with human HSC (CD34+ BM-derived cells from healthy adult donors, purchased from AllCells, LLC, or SaveFull Paper
2018Sebastian, AA;Kannan, TP;Norazmi, MN;Nurul, AA;Interleukin-17A promotes osteogenic differentiation by increasing OPG/RANKL ratio in stem cells from human exfoliated deciduous teeth (SHED)Journal of tissue engineering and regenerative medicine29774992SHED were purchased from ALLCELLS, Alameda, CA, USA (Catalogue No. DP004F; lot number SHED092211-01). SHED from Passages 4 to 6 were used in the study. SHED were cultured in alpha minimum essential medium (Gibco, USA) supplemented with 10% v/v fetalFull Paper
2018Gregg, KA;Harberts, E;Gardner, FM;Pelletier, MR;Cayatte, C;Yu, L;McCarthy, MP;Marshall, JD;Ernst, RK;A lipid A-based TLR4 mimetic effectively adjuvants a Yersinia pestis rF-V1 subunit vaccine in a murine challenge modelVaccine29861179Co-stimulatory marker surface expression assays were contracted to and completed by AllCells using standard protocols. Briefly, ex vivo human monocytes were differentiated to SaveFull Paper
2018Kanagasabai, R;Karmahapatra, S;Kientz, CA;Yu, Y;Hernandez, VA;Kania, EE;Yalowich, JC;Elton, TS;The Novel C-terminal Truncated 90-kDa Isoform of Topoisomerase IIα (TOP2α/90) Is a Determinant of Etoposide Resistance in K562 Leukemia Cells via Heterodimerization with the TOP2α/170 IsoformMolecular pharmacology29514855Human cord blood mononuclear cells were purchased from ALLCELLS (Alameda, CA). Deidentified acute myeloid leukemia (AML) patient samples were obtained from The Ohio State SaveFull Paper
2018Liu, L;Puri, N;Raffaele, M;Schragenheim, J;Singh, SP;Bradbury, JA;Bellner, L;Vanella, L;Zeldin, DC;Cao, J;Abraham, NG;Ablation of soluble epoxide hydrolase reprogram white fat to beige-like fat through an increase in mitochondrial integrity, HO-1-adiponectin in vitro and in vivoProstaglandins & other lipid mediators30041041Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA, USA). After thawing, mononuclear cells were re-suspended in a-minimal essential medium (a-MEM, Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat-in-aFull Paper
2018Wang, J;Xu, J;Peng, Y;Xiao, Y;Zhu, H;Ding, ZM;Hua, H;Phosphorylation of extracellular signal-regulated kinase as a biomarker for cannabinoid receptor 2 activationHeliyon30450437tal calf serum, 100 U/ml penicillin/streptomycin, and 2 mM l-glutamine at 37 °C in 5% CO2/95% air. 2.2 Human whole blood cell culture Human whole blood cells were purchased from commercial sources (Allcells, Alameda, CA, USA or Zen-Bio, Research TriFull Paper
2018Wiredja, DD;Tabler, CO;Schlatzer, DM;Li, M;Chance, MR;Tilton, JC;Global phosphoproteomics of CCR5-tropic HIV-1 signaling reveals reprogramming of cellular protein production pathways and identifies p70-S6K1 and MK2 as HIV-responsive kinases required for optimal infection of CD4+ T cellsRetrovirology29970186Normal donor samples were de-identified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated by SaveFull Paper
2018Friesen, WJ;Johnson, B;Sierra, J;Zhuo, J;Vazirani, P;Xue, X;Tomizawa, Y;Baiazitov, R;Morrill, C;Ren, H;Babu, S;Moon, YC;Branstrom, A;Mollin, A;Hedrick, J;Sheedy, J;Elfring, G;Weetall, M;Colacino, JM;Welch, EM;Peltz, SW;The minor gentamicin complex component, X2, is a potent premature stop codon readthrough molecule with therapeutic potentialPloS one30359426). Fold suppression over background was calculated as (aminoglycoside light units/PBS light units) [7]. Cytotoxicity assays Frozen human Peripheral Blood Mononuclear Cells (PBMCs) were obtained from ALLCELLS (Alameda, CA, Cat# PB003F) from a singleFull Paper
2018Caseiro, AR;Ivanova, G;Pedrosa, SS;Branquinho, MV;Georgieva, P;Barbosa, PP;Santos, JD;Magalhães, R;Teixeira, P;Pereira, T;Maurício, AC;Human umbilical cord blood plasma as an alternative to animal sera for mesenchymal stromal cells in vitro expansion - A multicomponent metabolomic analysisPloS one30304014, 29, 47-49]. UC-MSCs and DPSCs were maintained at 37°C and 95% humidified atmosphere with 5% CO2 environment. UC-MSCs were obtained from PromoCell, Cat. C-12971; Lot No. 1112304.2) and DPSCs from AllCells, LLC (Cat. DP0037F, Lot No. DPSC090411-01).Full Paper
2018Oshima, T;Miyashita, H;Ishimura, Y;Ito, Y;Tanaka, Y;Hori, A;Kokubo, T;Kurokawa, T;Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkeyPloS one29723247Human peripheral blood mononuclear cells (PBMC) purchased from AllCells, LLC and were cultured in RPMI1640 medium containing 10% fetal bovine serum, 0.1 nM human IL-2 ( SaveFull Paper
2018Del'Guidice, T;Lepetit-Stoffaes, JP;Bordeleau, LJ;Roberge, J;Théberge, V;Lauvaux, C;Barbeau, X;Trottier, J;Dave, V;Roy, DC;Gaillet, B;Garnier, A;Guay, D;Membrane permeabilizing amphiphilic peptide delivers recombinant transcription factor and CRISPR-Cas9/Cpf1 ribonucleoproteins in hard-to-modify cellsPloS one29617431N). Myoblasts are primary human cells kindly provided by Professor J.P. Tremblay (Université Laval, Quebec, QC, Canada). Normal Peripheral Blood CD56+ NK cells are natural killer cells obtained from Allcells Inc. (Alameda, CA, USA). Cortical cells arFull Paper
2018Sunderic, K;Effects of Thermal Dosing and Ultrasound on Osteogenic Differentiation of Human Mesenchymal Stem Cells in 2D and 3D CultureThesisHuman bone marrow explanted from the iliac crest of donors was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the RosetteSep MSC enrichment cocktail (StemCell Technologies, Vancouver, Canada) per manufacFull Paper
2018Nair, RR;Piktel, D;Geldenhuys, WJ;Gibson, LF;Combination of cabazitaxel and plicamycin induces cell death in drug resistant B-cell acute lymphoblastic leukemiaLeukemia research30103201Primary immune cells CD3 + T cells, CD19 + B cells, peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) were all purchased from ALLCELLS and SaveFull Paper
2018Palasuk, J;Windsor, LJ;Platt, JA;Lvov, Y;Geraldeli, S;Bottino, MC;Doxycycline-loaded nanotube-modified adhesives inhibit MMP in a dose-dependent fashionClinical oral investigations28965247Next, the eluates were serially diluted to 32-, 64-, and 128-fold [21]. Human dental pulp stem cells ([hDPSCs] AllCells LLC, Alameda, CA, USA) were cultured under standard conditions in a humidifier incubator (5% CO2/95% air) at 37 °C in low-glucoseFull Paper
2018Sasaki, E;Momose, H;Hiradate, Y;Ishii, KJ;Mizukami, T;Hamaguchi, I;In vitro marker gene expression analyses in human peripheral blood mononuclear cells: A tool to assess safety of influenza vaccines in humansJournal of immunotoxicology29521144PBMC obtained from healthy adult subjects were purchased from AllCells (Alameda, CA) and cultured in complete RPMI, ie RPMI 1640 supplemented with 10% fetal calf serum (FCS), SaveFull Paper
2018Tian, H;Li, S;Yu, K;DJ‑1 alleviates high glucose‑induced endothelial cells injury via PI3K/Akt‑eNOS signaling pathwayMolecular medicine reports29115508HUVECs were purchased from AllCells Biotechnology Co., Ltd. (Shanghai, China). The culture medium was DMEM with either 5.5 mM (control group) or 25 mM (HG group) D-glucose, containing 10% fetal bovine serum, penicillin (100 U/ml) and streptomycin (10Full Paper
2018Hu, X;Hu, T;Guan, G;Yu, S;Wu, Y;Wang, L;Control of weft yarn or density improves biocompatibility of PET small diameter artificial blood vesselsJournal of biomedical materials research. Part B, Applied biomaterials28456004The HUVECs (HUVEC001), provided by Allcells Company (Shanghai, China), were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Gibco) at 37°C in a 5% CO2 incubator. Before seFull Paper
2018Joslin, J;Gilligan, J;Anderson, P;Garcia, C;Sharif, O;Hampton, J;Cohen, S;King, M;Zhou, B;Jiang, S;Trussell, C;Dunn, R;Fathman, JW;Snead, JL;Boitano, AE;Nguyen, T;Conner, M;Cooke, M;Harris, J;Ainscow, E;Zhou, Y;Shaw, C;Sipes, D;Mainquist, J;Lesley, S;A Fully Automated High-Throughput Flow Cytometry Screening System Enabling Phenotypic Drug DiscoverySLAS discovery : advancing life sciences R & D29843542Primary human CD4 T cells were purified using a CD4+ T-cell isolation kit (130-096-533, Miltenyi, Auburn, CA) from cells obtained from leukapheresis of a normal human donor (AllCells Save;reg (T-Regulatory) Screen Primary human CD4 T cells were puriFull Paper
2018Barve, A;Casson, L;Krem, M;Wunderlich, M;Mulloy, JC;Beverly, LJ;Comparative utility of NRG and NRGS mice for the study of normal hematopoiesis, leukemogenesis, and therapeutic responseExperimental hematology30125602Cell-line-derived xenografts (CDXs) or patient-derived xenografts (PDXs) in immune-deficient mice have revolutionized our understanding of normal and malignant human SaveFull Paper
2018Reshetnikova, E;Guselnikov, S;Volkova, O;Baranov, K;Taranin, A;Mechetina, L;B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humansCytometry. Part B, Clinical cytometry29236355For this purpose, pDC cDNA from the BDCA4+ (CD304+) pDCs isolated from the peripheral blood of a healthy donor (cat. no. cDNA-PB006) was purchased from AllCells, LLC (Alameda, CA, USA). Human PBMC and spleen total RNA was isolated using Qiagen RNAeasFull Paper
2018Ikutani, M;Ogawa, S;Yanagibashi, T;Nagai, T;Okada, K;Furuichi, Y;Takatsu, K;Elimination of eosinophils using anti-IL-5 receptor alpha antibodies effectively suppresses IL-33-mediated pulmonary arterial hypertrophyImmunobiology29269115Human PBMCs (AllCells, Alameda, CA, USA) and Y16 cells were used as effector cells and target cells, respectively. Target cells (2 × 104 cells) were incubated with the effector cells (5 × 105 cells) and chimeric antibodies in a 96-well U-form plate aFull Paper
2018Sun, K;Mikule, K;Wang, Z;Poon, G;Vaidyanathan, A;Smith, G;Zhang, ZY;Hanke, J;Ramaswamy, S;Wang, J;A comparative pharmacokinetic study of PARP inhibitors demonstrates favorable properties for niraparib efficacy in preclinical tumor modelsOncotarget30647846m to assess human megakaryocyte-specific development In vitro human megakaryocyte differentiation was assessed using a liquid culture system in which the human bone marrow CD34+ cells (lot # 0150114, ReachBio) derived from normal bone marrow were useFull Paper
2018Liao, C;Regulation of Stress Erythropoiesis and the Niche: A Noval Role of SelenoproteinsThesisTransferrin (200µg/mL), Scf (50ng/mL), Bmp4 (15ng/mL), Gdf15 (30ng/mL), and Shh (25ng/mL), for 5 to 7 days to enrich for SEPs. Human BM cells from three subjects were purchased from Reachbio Research Labs. Human BM culture was the same condition withFull Paper
2018Wu, H;He, L;Shi, J;Hou, X;Zhang, H;Zhang, X;An, Q;Fan, F;Resveratrol inhibits VEGF-induced angiogenesis in human endothelial cells associated with suppression of aerobic glycolysis via modulation of PKM2 nuclear translocationClinical and experimental pharmacology & physiology30044005medium (DMEM) (Hyclone, Pittsburgh, PA, USA) supplemented with 10% foetal bovine serum (FBS; Cellmax, Beijing, China), 100 U/mL penicillin and 100 μg/mL streptomycin, and grown in a 95% air and 5% CO2 humidified atmosphere at 37°C, Primary HUVECs werFull Paper
2018Ni, R;Luo, L;A noncanonical function of histidyl-tRNA synthetase: inhibition of vascular hyperbranching during zebrafish developmentFEBS open bio29744287loheximide (CHX; Sigma) from 36 hpf to observation, or treated with DMSO at the same concentration as control. Immunofluorescence staining and imaging Human umbilical vein endothelial cells (HUVECs, ALLCELLS) were cultured according to standard protFull Paper
2018Han, XB;Tan, Y;Fang, YQ;Li, F;Protective effects of celastrol against γ irradiation-induced oxidative stress in human umbilical vein endothelial cellsExperimental and therapeutic medicine30116323anghai Xingya Purify Material Ltd. (Shanghai, China). Western blotting detection ECL Reagent was purchased from GE Healthcare China Co. Ltd. (Beijing, China). Cell culture HUVECs were purchased from AllCells Biotech Ltd. (Shanghai, China), and grownFull Paper
2018Varma, DM;DiNicolas, MS;Nicoll, SB;Injectable, redox-polymerized carboxymethylcellulose hydrogels promote nucleus pulposus-like extracellular matrix elaboration by human MSCs in a cell density-dependent mannerJournal of biomaterials applications30326804hMSCs were isolated from adult human bone marrow (AllCells, Emeryville, CA) via enriched density gradient centrifugation as previously described. Briefly, a hMSC Enrichment SaveFull Paper
2018Zhao, X;Gao, J;Hu, X;Guo, H;Wang, F;Qiao, Y;Wang, L;Collagen/Polyethylene Oxide Nanofibrous Membranes with Improved Hemostasis and Cytocompatibility for Wound DressingApplied SciencesHuman umbilical vein endothelial cells (HUVEC001) were supplied by Allcells Company (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS, Gibco, Shanghai, China) and 1% penicillin-streptomycin (Full Paper
2018Thomson-Luque, R;Wang, C;Ntumngia, FB;Xu, S;Szekeres, K;Conway, A;Adapa, SR;Barnes, SJ;Adams, JH;Jiang, RHY;In-depth phenotypic characterization of reticulocyte maturation using mass cytometryBlood cells, molecules & diseases30007855). Reticulocytes and mature RBCs were kept at 4 °C in MIM until used, always before 2 days post isolation. One bone marrow reticulocyte preparation was obtained from unprocessed whole aspirates (ALLCELLS) following the same protocol as for peripheralFull Paper
2018Wang, J;Xu, J;Liu, J;Zhu, H;Peng, Y;Ding, ZM;Hua, H;Genetic Variant Q63R of Cannabinoid Receptor 2 Causes Differential ERK Phosphorylation in Human Immune CellsGenetic testing and molecular biomarkers29694791All human PBMCs were from commercial sources (provided by Allcells or Zenbio). Human PBMCs were thawed and cultured in the culture medium (RPMI-1640 containing 2 mM L- SaveFull Paper
2018Gao, X;Yourick, JJ;Sprando, RL;Generation of nine induced pluripotent stem cell lines as an ethnic diversity panelStem cell research30099336Whole blood collection from healthy donors was conducted by AllCells with informed consent under the Protocol #7000-SOP-049 Version 2.0 approved by Alpha Independent Review SaveFull Paper
2018Pan, H;Zhang, M;Serum of coronary atherosclerotic heart disease patients induces oxidative stress injury on endothelial cellsPteridinesHUVECs were purchased from AllCells (AllCells Technology, Shanghai, China). Cells were grown in complete medium (HUVEC-004, AllCells) in an incubator with 5% CO2 at 37 °C. HUVECs were treated with the serum from patients with CAD for 24 h. HUVECs werFull Paper
2018Enezei, H;Ahmad, A;Takeuchi, K;Suzuki, J;Khamis, M;Razak, N;Rahman, R;Qabbani, A;Abdulhameed, E;Sugita, Y;Maeda, H;Alam, M;Osteoinductive Activity of Bone Scaffold Bioceramic Companied with Control Release of VEGF Protein Treated Dental stem cells as A New Concept for Bone Regeneration: Part IIJournal of Hard Tissue BiologyDental Stem Cells (DSCs) AllCells, LLC Company, Emeryville, CA, USA Dulbecco’s modified Eagles medium (DMEM) high glucose(4.5g/L) Invitrogen, (GIBCO), Faraday Ave, Carlsbad, CA, USA Fetal bovine serum (FBS) Invitrogen, (GIBCO), Faraday Ave, Carlsbad,Full Paper
2018Sasaki, E;Momose, H;Hiradate, Y;Furuhata, K;Mizukami, T;Hamaguchi, I;Development of a preclinical humanized mouse model to evaluate acute toxicity of an influenza vaccineOncotarget29899819Human PBMCs from one healthy donor (24 years old, male adult Caucasian) were obtained from AllCells (Alameda, CA, USA) and were subjected to the experiment shown in Figures 1 SaveFull Paper
2018Gołąb, K;Grose, R;Placencia, V;Wickrema, A;Solomina, J;Tibudan, M;Konsur, E;Ciepły, K;Marek-Trzonkowska, N;Trzonkowski, P;Millis, JM;Fung, J;Witkowski, P;Cell banking for regulatory T cell-based therapy: strategies to overcome the impact of cryopreservation on the Treg viability and phenotypeOncotarget29515766We obtained de-identified human cell leukapheresis product for all the experiments from the commercial vendor AllCells, Alameda, CA, USA, therefore the study was exempt from the SaveFull Paper
2018Shehadat, SA;Gorduysus, MO;Hamid, SSA;Abdullah, NA;Samsudin, AR;Ahmad, A;Optimization of scanning electron microscope technique for amniotic membrane investigation: A preliminary studyEuropean journal of dentistry30369805que by a cell scraper (TPP, Europe/Switzerland) was applied when necessary to remove the epithelial cells that could not be washed away by phosphate-buffered saline (PBS). Deciduous teeth stem cells (AllCells, USA) were seeded on the de-epithelializeFull Paper
2018Subhi, H;Reza, F;Husein, A;Al Shehadat, SA;Nurul, AA;Gypsum-Based Material for Dental Pulp Capping: Effect of Chitosan and BMP-2 on Physical, Mechanical, and Cellular PropertiesInternational journal of biomaterials30147725SHED were purchased from AllCells, USA. The cells at passage 8 were cultured in Alpha minimum essential medium [????-MEM; Invitrogen, USA (GIBCO)] supplemented with 10% fetal SaveFull Paper
2018Ross, CL;Pettenati, MJ;Procita, J;Cathey, L;George, SK;Almeida-Porada, G;Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal CellsGlobal advances in health and medicine29796339me stability were not affected or altered by ELF-EMF, when used at the tested field strength and frequency. Materials and Methods Cell Culture Human bone mononuclear cells (BMNCs) were obtained from AllCells (Alameda, CA). BMNCs were enriched for thFull Paper
2018Subhi, H;Reza, F;Husein, A;Nurul, AA;Cytotoxicity of gypsum-based biomaterial for direct pulp capping using stem cells from human exfoliated deciduous teethJournal of conservative dentistry : JCD29628642epare the biomaterial. The compositions of the Gyp-CHT biomaterial are shown in Table 1. Table 1 The compositions of the experimental biomaterial Cell culture preparation SHED were purchased from AllCells, USA. The cells at passage eight were culFull Paper
2018Luddin, N;Ahmed, H;Kannan, T;Mokhtar, K;Ahmad, A;Calcium chloride dihydrate affects the biological properties of white mineral trioxide aggregate on dental pulp stem cells: An in vitro studySaudi Endodontic Journalmolars (adult) and characterized were purchased from AllCells (CA, USA). The complete (AllCells), with 50 ml of human mesenchymal stem cell stimulatory supplements (AllCells). SaveFull Paper
2018Goncalves, K;Hoban, M;Proctor, J;Hyzy, S;Adams, H;Gardner, J;Boitano, A;Cooke, M;Comparison of hematopoietic stem cell expansion methods reveals that aryl hydrocarbon receptor inhibition optimally expands CD90 engrafting cellsSSRN Electronic JournalHuman cord blood CD34+ cells or mobilized peripheral blood CD34+ cells (male or female) were purchased from AllCells. Cell number, purity, and viability was confirmed at time of thaw. Cell counts were obtained using TruCount Tubes (BD). Cell viabilitFull Paper
2018laura, ;Danilevsky, M;Li, Y;Reiss, F;Zhu, H;SystemT: Declarative Text Understanding for EnterpriseProceedings of the 2018 Conference of the North American Chapter of the Association for Computational Linguistics: Human Language Technologies, Volume 3 (Industry Papers)able in AQL, including dictionary matching (AssetClassSuffixes), core NLP operators such as Semantic Role Labeling (SRL) (Verbs, Arguments), and document structure operators such as table structure understanding (AllCells). Sec. 3 details the data moFull Paper
2018Abhyankar, V;The chemokine and cytokine responses of a keratinocyte, dendritic cell, and T-cell co-culture model treated with P. gingivalis hemagglutinin B (HagB)University of IowaBackground: P. gingivalis, a non-motile, rod-shaped, anaerobic, Gram-negative bacterium is one of the principal sources of periodontal disease. It possesses a number of potential SaveFull Paper
2018Sabhachandani, ;Development of microfluidic microanalysis systems for cancer therapeutic screeningNortheastern Universitycells were purchased from AllCells (#PB009-3F, AllCells, Alameda, CA). Cells were maintained in RPMI-1640 medium supplemented with 10% Fetal Bovine Serum and 1% Antibiotic-Antimycotic solution (Corning Cellgro, Manassas, VA). Primary human CD56+ NK cFull Paper
2018Bates, A;Matrix metalloproteinase and cytokine profiles from cell co-cultures and their role in oral inflammation and head and neck cancerUniversity of IowaHuman monocyte-derived immature myeloid dendritic cells (DC, AllCells, Alameda, CA) were positively identified by flow cytometry and contained DC of myeloid origin: 31.5% CD1c+/79.1% CD11c+ cells and 54.4% CD141+ /79.1% CD11c+ cells (Human myeloid DCFull Paper
2018Nada, M;Adoptive cancer immunotherapy with human Vγ2vδ2 T cellsUniversity of IowaEx vivo expansion of Vγ2Vδ2 T cells PBMC from random healthy adult donors were obtained by density centrifugation over Ficoll-Hypaque (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) of either random donor leukopaks (12 donors) (obtained from AllCeFull Paper
2018Twyman, RM;Wisden, W;Development, Molecular AspectsAdvance Molecular BiologyA fate map is a map of an egg or embryo which shows the fates of allcells A cell which will form gametes. Germ cells usually segregate from somatic cells early in animal development SaveFull Paper
2018Riepe, C;Zelin, E;Wyman, S;Nguyen, D;Liang, J;Frankino, P;Meacham, Z;Vu, J;Marson, A;Ingolia, N;Corn, J;Double Stranded DNA Breaks and Genome Editing Trigger Ribosome Remodeling and Translational ShutdownbioRxivMobilized Peripheral Blood CD34+ Stem/Progenitor Cells (AllCells) were cultured in StemSpan™ Serum Free Expansion Media II (STEMCELL Technologies), StemSpan™ StemSpan(tm) CC110 (STEMCELL Technologies), 1% Pen/StrepFull Paper
2018Mimitou, E;Cheng, A;Montalbano, A;Hao, S;Stoeckius, M;Legut, M;Roush, T;Herrera, A;Papalexi, E;Ouyang, Z;Satija, R;Sanjana, N;Koralov, S;Smibert, P;Expanding the CITE-seq tool-kit: Detection of proteins, transcriptomes, clonotypes and CRISPR perturbations with multiplexing, in a single assaybioRxivDMSO and aliquots of 1-1.5 million cells were thawed on the day of the experiment. Peripheral blood mononuclear cells (PBMCs) were obtained cryopreserved from AllCells (USA) and used immediately after thawing. NIH-3T3 (mouse) cells expressing non-tarFull Paper
2018Setty, M;Kiseliovas, V;Levine, J;Gayoso, A;Mazutis, L;Pe’er, D;Palantir characterizes cell fate continuities in human hematopoiesisbioRxivCryopreserved bone marrow stem/progenitor CD34+ cells from healthy donors were purchased from AllCells, LLC. (Cat. No. ABM022F) and stored in vapor phase nitrogen until use. Typical for scRNA-seq, a vial was removed from the storage and immediately tFull Paper
2018Shin, J;Wyman, S;Dewitt, M;Bray, N;Vu, J;Corn, J;Controlled cycling and quiescence enables homology directed repair in engraftment-enriched adult hematopoietic stem and progenitor cellsbioRxivCryopreserved wildtype human mobilized peripheral blood CD34+ HSPCs from multiple volunteer donors including male and female whose age ranged from 20-35 were purchased from Allcells, IncFull Paper
2018Wong, D;Immuno-Chemotherapeutic Platinum(IV) Prodrugs of Cisplatin as Multimodal Anticancer AgentsRethinking Platinum Anticancer Drug Design: Towards Targeted and Immuno-chemotherapeutic ApproachesPrior to activation, frozen PBMCs (AllCells LLC, USA) were thawed and washed twice with complete RPMI to remove freezing medium. 4.1 × 10 6 PBMCs were incubated in 5 mL of SaveFull Paper
2018Johnsen, KH;Performance Analysis and Optimization of the Equelle DSLThesisGradient() takes a CollOfScalar as argument which is defined on AllCells and returns a CollOfScalar which is defined on InteriorFaces. The Divergence() function is used to calculate the SaveFull Paper
2018Ganaie, SS;Essential Host Factors for Human Parvovirus B19 ReplicationThesisWe purchased CD34+ hematopoietic stem cells, which were isolated from bone marrow of a healthy human donor, from AllCells LLC (Alameda, CA) without any identification information on the cells, and, therefore, an institutional review board (IRB) revieFull Paper
2018Fink, EC;Investigating the Mechanism and Species-Specificity of Thalidomide DerivativesThesisControl cord-blood CD34+ cells were isolated as above. Adult bone marrow CD34+ cells were purchased as single-donor lots from AllCells (Alameda, CA). The number of replicates for each patient sample, vector, and treatment was limited by the number ofFull Paper
2018Ahmed, MA;Exploring the impact of hypoxia mimetic agents on multipotent stem cell biologyThesisFresh human bone marrow aspirate ABM001-1 ALLCELLS Gel Loading Buffer (0.05% bromophenol blue, 40% sucrose, 0.1 M EDTA, 0.5% SDS) G2526 Sigma-Aldrich Glycine 50046 SIGMA-ALDRICH Horse serum H 1270 Sigma-Aldrich GAP-43 Antibody NB300-143 Novus BiologiFull Paper
2018Sebastian Kettinger, N;Investigating the HIV Reservoir in Hematopoietic Stem and Progenitor CellsThesis(eBioscience), and 7-Aminoactinomycin D (7-AAD). Healthy donor BMMCs (AllCells) were stained with R-phycoerythrin-conjugated anti-CD133, R-phycoerythrin-Cy7-conjugated anti-CD34 (BD), and 7-AAD. Samples were analyzed on a BD FACSCanFull Paper
2018Al-Jumaily, RMK;Molecular cytology of hypoxic cancer and stem cells: an epigenetic approachThesis1 BMA-16 Human Bone Marrow, Male, Age-29 years Lonza, MD, USA 2 BMA-20 Human Bone Marrow, Female, Age-50 years AllCells, LLC, SA 3 BMA-25 Human Bone Marrow, Male, Age-27 years Lonza, MD, USAFull Paper
2018Becker, AH;Epigenetic and Gene Expression Changes Mediated by Histone H3 Methylation in Acute Myeloid LeukemiaThesis2.1.2 Bone marrow CD34+ cells Cryopreserved bone marrow CD34+ cells from normal donors were obtained from Allcells (Alameda, CA). 2.1.3 K562 cells Human K562 cells (ATCC CCL-243) were grown in RPMI 1640 GlutaMAX medium (Gibco, Thermo Fisher ScientifiFull Paper
2018Bartenstein, M;Symptomatic treatment of myelofibrosis by TGF-β1 signaling inhibitionThesisUniversity of Chicago. Cells were purified immune-magnetically from granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood of healthy donors purchased from AllCells, Inc. using a paramagnetically labeled mouse anti-human CD34 antiboFull Paper
2018Siriwon, N;Engineered CAR-T Cells for Treatment of Solid CancersThesisFrozen human PBMCs were obtained from AllCells (Alameda, CA). PBMCs were thawed in TCM and rested overnight. Before retroviral transduction, PBMCs were activated for 2 days by culturing with Dynabeads human T-activator CD3/CD28 (Thermo Fisher ScientiFull Paper
2018Seet, C;In Vitro Generation of Adaptive Immunity from Hematopoietic Stem CellsThesisdeliveries at UCLA. Bone marrow (BM) was obtained from healthy adult donors (ages 18-51) through discarded material from allogeneic BM donor harvests at UCLA or purchased from AllCells Inc. (Alameda, CA). G-CSF mobilized peripheral blood was obtainedFull Paper
2018Lin, HA;Development and Functional Evaluation of Injectable Cellulose-based Hydrogels as Nucleus Pulposus Replacements for Intervertebral Disc RepairThesis2.2.4. Cell-laden hydrogel fabrication and characterization 2.2.4.1. Primary cell isolation Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density gradient centrifugation as previously described (Gupta et al., 201Full Paper
2018Marvin, CM;Spatial and Temporal Delivery of Small Molecules from Long Wavelength Sensitive Phototherapeutcs Using Erythrocytes as Biological CarriersThesis525 nm and 660 nm LEDs were purchased from LED Supply. 725 nm and 780 nm LEDs were purchased from Techmark. Erythrocytes were purchased from Allcells. HRMS data was acquired on a Thermo Scientific electrospray linear trap quadrupole fourier transformFull Paper
2018Hu, C;Data-Driven Discovery of Proteomic Hallmarks in Acute Myeloid LeukemiaThesisThe samples were normalized to a concentration of 1x104 cells/µL and a whole cell lysate was prepared as described in publications [103, 36]. In addition to AML patient samples, each array includes the same ten cryopreserved normal bone marrow CD34+Full Paper
2018Sun, V;Engineering Solutions for Biomaterials: Self-Assembly and Surface-Modification of Polymers for Clinical ApplicationsThesisHuman peripheral blood mononuclear cells (PBMCs) were obtained from AllCells (Alameda, CA). Frozen PBMCs were thawed and cultured in TCM containing X-VIVO™ 15 serum-free medium (Lonza, Allendale NJ), 5% (vol/vol) GemCell human serum antibody AB (GemiFull Paper
2018Weng, H;Huang, H;Wu, H;Qin, X;Zhao, BS;Dong, L;Shi, H;Skibbe, J;Shen, C;Hu, C;Sheng, Y;Wang, Y;Wunderlich, M;Zhang, B;Dore, LC;Su, R;Deng, X;Ferchen, K;Li, C;Sun, M;Lu, Z;Jiang, X;Marcucci, G;Mulloy, JC;Yang, J;Qian, Z;Wei, M;He, C;Chen, J;METTL14 Inhibits Hematopoietic Stem/Progenitor Differentiation and Promotes Leukemogenesis via mRNA m6A ModificationCell stem cell29290617were then co-transduced with different combinations of retroviruses or lentiviruses as indicated through two rounds of “spinoculation”. Cells were then plated into ColonyGEL methylcellulose medium (ReachBio, Seattle, WA) supplied with 10 ng/ml of murFull Paper
2018Probst, K;Stermann, J;von Bomhard, I;Etich, J;Pitzler, L;Niehoff, A;Bluhm, B;Xu, HC;Lang, PA;Chmielewski, M;Abken, H;Blissenbach, B;Machova, A;Papadopoulou, N;Brachvogel, B;Depletion of Collagen IX Alpha1 Impairs Myeloid Cell FunctionStem cells (Dayton, Ohio)30063808Flushed bone marrow samples from 2 months old mice were resuspended in Iscove's Modified Dulbecco's Medium containing 10% FCS and used for the colony forming unit assay (ColonyGEL 1203, Reachbio, Seattle)Full Paper
2018Li, G;Boucher, JC;Kotani, H;Park, K;Zhang, Y;Shrestha, B;Wang, X;Guan, L;Beatty, N;Abate-Daga, D;Davila, ML;4-1BB enhancement of CAR T function requires NF-κB and TRAFsJCI insight30232281Human peripheral blood mononuclear cells from healthy donors were purchased from ReachBio. Human T cell complete medium consists of RPMI1640 medium, 10% FBS, 2 mM L- SaveFull Paper
2018Kong, J;Han, L;Su, H;Hu, Y;Huang, X;Lou, Y;Riligustilide Attenuated Renal Injury by the Blockade of ReninCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology30308499Mouse anti-CBP monoclonal antibody SC-7300; rabbit anti-CREB polyclonal antibody SC-186 were commercially purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA;). CY5 was obtained from ReachBio WA, USA (#111-165-003), and Alexa Fluor® 488 wasFull Paper
2018Psatha, N;Reik, A;Phelps, S;Zhou, Y;Dalas, D;Yannaki, E;Levasseur, DN;Urnov, FD;Holmes, MC;Papayannopoulou, T;Disruption of the BCL11A Erythroid Enhancer Reactivates Fetal Hemoglobin in Erythroid Cells of Patients with β-Thalassemia MajorMolecular therapy. Methods & clinical development30182035anulocyte macrophage [GM] and burst-forming unit-erythroid [BFU-E]), cells were plated at a density of 750-2,000 cells/mL in semi-solid methylcellulose-based medium containing cytokines, ColonyGEL (ReachBio). Zinc Finger Nucleases ZFN pairs were expFull Paper
2018Li, C;Psatha, N;Gil, S;Wang, H;Papayannopoulou, T;Lieber, A;HDAd5/35++ Adenovirus Vector Expressing Anti-CRISPR Peptides Decreases CRISPR/Cas9 Toxicity in Human Hematopoietic Stem CellsMolecular therapy. Methods & clinical development30038942ng/mL SCF, 3 IU/mL EPO, 10 μg/mL insulin, 330 μg/mL transferrin, 2 U/mL heparin, and 1 μg/mL DOX for 6 days. CFU Assay CFU assays with transduced CD34+ cells were performed using ColonyGEL (Reachbio, Seattle, WA, USA) with human complete medium accoFull Paper
2018Wang, H;Richter, M;Psatha, N;Li, C;Kim, J;Liu, J;Ehrhardt, A;Nilsson, SK;Cao, B;Palmer, D;Ng, P;Izsvák, Z;Haworth, KG;Kiem, HP;Papayannopoulou, T;Lieber, A;A Combined In Vivo HSC Transduction/Selection Approach Results in Efficient and Stable Gene Expression in Peripheral Blood Cells in MiceMolecular therapy. Methods & clinical development29255741Biotec, San Diego, CA, USA) was used in accordance with the manufacturer’s instructions. CFU Assay 1,500 lineage−, GFP+ cells were plated in triplicates in ColonyGEL 1202 mouse complete medium (ReachBio, Seattle, WA, USA) and incubated for 12 days aFull Paper
2017Lim, JS;Ibaseta, A;Fischer, MM;Cancilla, B;O'Young, G;Cristea, S;Luca, VC;Yang, D;Jahchan, NS;Hamard, C;Antoine, M;Wislez, M;Kong, C;Cain, J;Liu, YW;Kapoun, AM;Garcia, KC;Hoey, T;Murriel, CL;Sage, J;Intratumoural heterogeneity generated by Notch signalling promotes small-cell lung cancerNature28489825... Luminex assay for midkine protein in human plasma Human plasma samples from cancer-free normal donors were purchased from BioreclamationIVT. SCLC donor plasma was sourced from Conversant Biologics (Conversant Bio). The samples were collected, pFull Paper
2017Alley, EW;Lopez, J;Santoro, A;Morosky, A;Saraf, S;Piperdi, B;van Brummelen, E;Clinical safety and activity of pembrolizumab in patients with malignant pleural mesothelioma (KEYNOTE-028): preliminary results from a non-randomised, open-label, phase 1b trialLancet Oncol.28291584PD-L1 expression was assessed in archival or new tumour samples by a central laboratory (QualTek Molecular Laboratories, Goleta, CA, USA) with a prototype assay and the 22C3 antibody (Merck, Kenilworth, NJ, USA).22, 23 PD-L1 positivity was defined byFull Paper
2017Plimack, ER;Bellmunt, J;Gupta, S;Berger, R;Chow, LQ;Juco, J;Lunceford, J;Saraf, S;Perini, RF;O'Donnell, PH;Safety and activity of pembrolizumab in patients with locally advanced or metastatic urothelial cancer (KEYNOTE-012): a non-randomised, open-label, phase 1b studyLancet Oncol.28081914Initially, we used a prototype PD-L1 immunohistochemical assay to establish the eligibility of patients in KEYNOTE-012, which was done at a laboratory site at QualTek (Goleta, CA, USA) accredited by the College of American Pathologists and Clinical LFull Paper
2017Frenel, JS;Le Tourneau, C;O'Neil, B;Ott, PA;Piha-Paul, SA;Gomez-Roca, C;van Brummelen, EMJ;Rugo, HS;Thomas, S;Saraf, S;Rangwala, R;Varga, A;Safety and Efficacy of Pembrolizumab in Advanced, Programmed Death Ligand 1-Positive Cervical Cancer: Results From the Phase Ib KEYNOTE-028 TrialJ. Clin. Oncol.29095678tumor sample or newly obtained core or excisional biopsy sample, was defined as membranous staining on $ 1% modified proportion score or interface pattern as assessed using a laboratory-developed prototype immunohistochemistry assay (QualTek MoleculaFull Paper
2017Hsu, C;Lee, SH;Ejadi, S;Even, C;Cohen, RB;Le Tourneau, C;Mehnert, JM;Algazi, A;van Brummelen, EMJ;Saraf, S;Thanigaimani, P;Cheng, JD;Hansen, AR;Safety and Antitumor Activity of Pembrolizumab in Patients With Programmed Death-Ligand 1-Positive Nasopharyngeal Carcinoma: Results of the KEYNOTE-028 StudyJ. Clin. Oncol.28837405Tumor PD-L1 positivity was established at a central laboratory at baseline on an archived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy sample using a laboratory-developed prototype immunohistochemical assay (QualTek MolecFull Paper
2017Ott, PA;Elez, E;Hiret, S;Kim, DW;Morosky, A;Saraf, S;Piperdi, B;Mehnert, JM;Pembrolizumab in Patients With Extensive-Stage Small-Cell Lung Cancer: Results From the Phase Ib KEYNOTE-028 StudyJ. Clin. Oncol.28813164Tumor PD-L1 expression was required to be eligible for treatment in the study; it was evaluated in archival or fresh tumor samples by a central laboratory (QualTek Molecular Laboratories, Goleta, CA) by using a prototype assay and the 22C3 antibody (Full Paper
2017Chen, R;Zinzani, PL;Fanale, MA;Armand, P;Johnson, NA;Brice, P;Radford, J;Ribrag, V;Molin, D;Vassilakopoulos, TP;Tomita, A;von Tresckow, B;Shipp, MA;Zhang, Y;Ricart, AD;Balakumaran, A;Moskowitz, CH;, ;Phase II Study of the Efficacy and Safety of Pembrolizumab for Relapsed/Refractory Classic Hodgkin LymphomaJ. Clin. Oncol.28441111PD-L1 expression was determined as previously described, 16 using fresh or archival formalin-fixed, paraffin-embedded pretreatment tissue sectioned at 4 to 5 microns, with a proprietary immunohistochemical assay developed at QualTek Molecular LaboratFull Paper
2017Colombo, I;Lien, S;Yang, C;Clouthier, D;Bonilla, L;Cyriac, S;Ethier, J;Lee, Y;Kanjanapan, Y;Mandilaras, V;Dhani, N;Butler, M;Oza, A;Quintos, J;Chow, H;Pugh, T;Ohashi, P;Siu, L;Lheureux, S;Immunologic and genomic characterization of high grade serous ovarian cancer (HGSOC) in patients (pts) treated with pembrolizumab (Pembro) in the phase II INSPIRE trial.JCOMean Tumor Proportion Score of PD-L1 by IHC (Qualtek) was 6.4% (range 0-30%). Grade 3/4 adverse events possibly related to Pembro were observed in 4/18 (22%) pts; none was fatal and the most common were fatigue and hyponatremiaFull Paper
2017Toy, W;Weir, H;Razavi, P;Lawson, M;Goeppert, AU;Mazzola, AM;Smith, A;Wilson, J;Morrow, C;Wong, WL;De Stanchina, E;Carlson, KE;Martin, TS;Uddin, S;Li, Z;Fanning, S;Katzenellenbogen, JA;Greene, G;Baselga, J;Chandarlapaty, S;Activating ESR1 Mutations Differentially Affect the Efficacy of ER AntagonistsCancer Discov27986707... n). PATIENT-DERIVED XENOGRAFTS (PDX) CTC-714 PDX model was derived from patient circulating tumor cells (CTC) cultures, consented according to the Human Biological Samples Policy and purchased from Conversant Biologics. The CTCs were obtained fFull Paper
2017Li, J;Stagg, NJ;Johnston, J;Harris, MJ;Menzies, SA;DiCara, D;Clark, V;Hristopoulos, M;Cook, R;Slaga, D;Nakamura, R;McCarty, L;Sukumaran, S;Luis, E;Ye, Z;Wu, TD;Sumiyoshi, T;Danilenko, D;Lee, GY;Totpal, K;Ellerman, D;Hötzel, I;James, JR;Junttila, TT;Membrane-Proximal Epitope Facilitates Efficient T Cell Synapse Formation by Anti-FcRH5/CD3 and Is a Requirement for Myeloma Cell KillingCancer Cell28262555... ec). CD4+ or CD8+ cells were used as effectors in a 3:1 effector:target ratio. In Vitro Cytotoxicity Assay: Human Plasma Cells and Primary MM Samples Human BMMCs from MM patients were procured from Conversant Bio. Human bone marrow aspirates ofFull Paper
2017Le Pichon, CE;Meilandt, WJ;Dominguez, S;Solanoy, H;Lin, H;Ngu, H;Gogineni, A;Sengupta Ghosh, A;Jiang, Z;Lee, SH;Maloney, J;Gandham, VD;Pozniak, CD;Wang, B;Lee, S;Siu, M;Patel, S;Modrusan, Z;Liu, X;Rudhard, Y;Baca, M;Gustafson, A;Kaminker, J;Carano, RAD;Huang, EJ;Foreman, O;Weimer, R;Scearce-Levie, K;Lewcock, JW;Loss of dual leucine zipper kinase signaling is protective in animal models of neurodegenerative diseaseSci Transl Med28814543AD (early-stage AD) or confirmed AD along with age-matched controls from Folio Biosciences or Banner Sun Health Research Institute. All human tissues were collected with informed consents and institutional review board approvals.Full Paper
2017Pearlman, R;Frankel, WL;Swanson, B;Zhao, W;Yilmaz, A;Miller, K;Bacher, J;Bigley, C;Nelsen, L;Goodfellow, PJ;Goldberg, RM;Paskett, E;Shields, PG;Freudenheim, JL;Stanich, PP;Lattimer, I;Arnold, M;Liyanarachchi, S;Kalady, M;Heald, B;Greenwood, C;Paquette, I;Prues, M;Draper, DJ;Lindeman, C;Kuebler, JP;Reynolds, K;Brell, JM;Shaper, AA;Mahesh, S;Buie, N;Weeman, K;Shine, K;Haut, M;Edwards, J;Bastola, S;Wickham, K;Khanduja, KS;Zacks, R;Pritchard, CC;Shirts, BH;Jacobson, A;Allen, B;de la Chapelle, A;Hampel, H;, ;Prevalence and Spectrum of Germline Cancer Susceptibility Gene Mutations Among Patients With Early-Onset Colorectal CancerJAMA Oncol27978560Myriad Genetics Inc donated the next-generation sequencing testing for mismatch repair–proficient patients with CRC diagnosed younger than 50 years for this study. Dr Kalady discloses honorarium from Helomics and Transenterix and is a member of the sFull Paper
2017Ott, PA;Piha-Paul, SA;Munster, P;Pishvaian, MJ;van Brummelen, EMJ;Cohen, RB;Gomez-Roca, C;Ejadi, S;Stein, M;Chan, E;Simonelli, M;Morosky, A;Saraf, S;Emancipator, K;Koshiji, M;Bennouna, J;Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with recurrent carcinoma of the anal canalAnn. Oncol.28453692mbedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek Molecular Laboratories, Goleta, CA) [15] uFull Paper
2017Hui, R;Garon, EB;Goldman, JW;Leighl, NB;Hellmann, MD;Patnaik, A;Gandhi, L;Eder, JP;Ahn, MJ;Horn, L;Felip, E;Carcereny, E;Rangwala, R;Lubiniecki, GM;Zhang, J;Emancipator, K;Roach, C;Rizvi, NA;Pembrolizumab as first-line therapy for patients with PD-L1-positive advanced non-small cell lung cancer: a phase 1 trialAnn. Oncol.28168303As previously described [5], PD-L1 expression for enrollment was assessed with a prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA) and the relationship between PD-L1 expression and efficacy was investigated using a cliFull Paper
2017Potts, AH;Vakulskas, CA;Pannuri, A;Yakhnin, H;Babitzke, P;Romeo, T;Global role of the bacterial post-transcriptional regulator CsrA revealed by integrated transcriptomicsNat Commun29150605;... nufacturer’s instructions with one modification. Adaptors and reverse transcription primers were diluted 1 in 5 in water before use. Libraries were sequenced using HiSeq 2500 50SE (Illumina) by the HudsonAlpha Genomic Services Laboratory. SequeFull Paper
2017Christopher, MA;Myrick, DA;Barwick, BG;Engstrom, AK;Porter-Stransky, KA;Boss, JM;Weinshenker, D;Levey, AI;Katz, DJ;LSD1 protects against hippocampal and cortical neurodegenerationNat Commun28993646... s possible, then RNA was precipitated with isopropanol. Pellets were then washed with 75% ethanol and resuspended in 50 µL deionized water. RNA library preparation and sequencing were performed by HudsonAlpha Genomic Services Lab. RNA was Poly(A)Full Paper
2017Ramaker, RC;Savic, D;Hardigan, AA;Newberry, K;Cooper, GM;Myers, RM;Cooper, SJ;A genome-wide interactome of DNA-associated proteins in the human liverGenome Res.29021291HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama 35806, USA.++HudsonAlpha Institute for BiFull Paper
2017Cubeñas-Potts, C;Rowley, MJ;Lyu, X;Li, G;Lei, EP;Corces, VG;Different enhancer classes in Drosophila bind distinct architectural proteins and mediate unique chromatin interactions and 3D architectureNucleic Acids Res.27899590New ChIP experiments were performed in Kc167 cells for GAF, H3, H3K27me3, Ibf1, Ibf2, Pita, Nup98 and ZIPIC as described previously with minor modifications (28). More precisely, antibody-bound protein complexes were isolated with Protein A or ProteiFull Paper
2017Edwardson, CF;Hollibaugh, JT;Metatranscriptomic analysis of prokaryotic communities active in sulfur and arsenic cycling in Mono Lake, California, USAISME J28548659synthesis kits. Libraries (~225 bp insert) were prepared using Illumina TruSeq technology. Samples were pooled and run on one lane of 150 × 2 Illumina HiSeq 2500 Rapid Run at HudsonAlpha Genomic Services. Reads haveFull Paper
2017Yeh, TC;O'Connor, G;Petteruti, P;Dulak, A;Hattersley, M;Barrett, JC;Chen, H;Identification of CCR2 and CD180 as Robust Pharmacodynamic Tumor and Blood Biomarkers for Clinical Use with BRD4/BET InhibitorsClin. Cancer Res.28073847Whole blood was collected in 10 mL EDTA vaccutainers (Becton Dickinson) from two control healthy volunteers and 11 patients with cancer with solid tumor or hematologic cancers through Conversant Bio. Compound incubation and sample processing was carrFull Paper
2017Kirtane, AR;Sadhukha, T;Kim, H;Khanna, V;Koniar, B;Panyam, J;Fibrinolytic Enzyme Cotherapy Improves Tumor Perfusion and Therapeutic Efficacy of Anticancer NanomedicineCancer Res.28108516... anticancer nanomedicine in syngeneic mouse melanoma and xenograft lung cancer models. Go to: MATERIALS AND METHODS MATERIALS Tissue microarrays of various human tumor biopsies were obtained from Folio Biosciences (Ohio, USA). Anti-human fibrFull Paper
2017Jung, YH;Sauria, MEG;Lyu, X;Cheema, MS;Ausio, J;Taylor, J;Corces, VG;Chromatin States in Mouse Sperm Correlate with Embryonic and Adult Regulatory LandscapesCell Rep28178516We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology, and specially Drs. Braden Boone, Angela Jones and Terri Pointer, for their help in performing Illumina sequencing of samples. This work was supported by UFull Paper
2017O'Daniel, JM;McLaughlin, HM;Amendola, LM;Bale, SJ;Berg, JS;Bick, D;Bowling, KM;Chao, EC;Chung, WK;Conlin, LK;Cooper, GM;Das, S;Deignan, JL;Dorschner, MO;Evans, JP;Ghazani, AA;Goddard, KA;Gornick, M;Farwell Hagman, KD;Hambuch, T;Hegde, M;Hindorff, LA;Holm, IA;Jarvik, GP;Knight Johnson, A;Mighion, L;Morra, M;Plon, SE;Punj, S;Richards, CS;Santani, A;Shirts, BH;Spinner, NB;Tang, S;Weck, KE;Wolf, SM;Yang, Y;Rehm, HL;A survey of current practices for genomic sequencing test interpretation and reporting processes in US laboratoriesGenet. Med.27811861Department of Genetics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.++Laboratory for Molecular Medicine, Partners Healthcare Personalized Medicine, Cambridge, Massachusetts, USA.++Division of Medical Genetics, UniverFull Paper
2017Rydzak, T;Garcia, D;Stevenson, DM;Sladek, M;Klingeman, DM;Holwerda, EK;Amador-Noguez, D;Brown, SD;Guss, AM;Deletion of Type I glutamine synthetase deregulates nitrogen metabolism and increases ethanol production in Clostridium thermocellumMetab. Eng.28400329; diluted. Sequencing was completed using a SR50 sequencing protocol and V4 chemistry on an Illumina HiSeq. 2500 platform (HudsonAlpha Genomic Services Laboratory; Huntsville, AL). 2.7. RNA-Seq analysis. Raw readsFull Paper
2017Tigue, NJ;Bamber, L;Andrews, J;Ireland, S;Hair, J;Carter, E;Sridharan, S;Jovanović, J;Rees, DG;Springall, JS;Solier, E;Li, YM;Chodorge, M;Perez-Martinez, D;Higazi, DR;Oberst, M;Kennedy, M;Black, CM;Yan, L;Schwickart, M;Maguire, S;Cann, JA;de Haan, L;Young, LL;Vaughan, T;Wilkinson, RW;Stewart, R;MEDI1873, a potent, stabilized hexameric agonist of human GITR with regulatory T-cell targeting potentialOncoimmunology28405505... xP3 Tissue samples from 76 cancer patients, with NSCLC, colorectal cancer (CRC), bladder cancer, or squamous cell cancer of the head and neck (SCCHN), were obtained from commercial sources (Asterand, ConversantBio, ProteoGenex, National Disease RFull Paper
2017Liu, Z;Zhang, C;Skamagki, M;Khodadadi-Jamayran, A;Zhang, W;Kong, D;Chang, CW;Feng, J;Han, X;Townes, TM;Li, H;Kim, K;Zhao, R;Elevated p53 Activities Restrict Differentiation Potential of MicroRNA-Deficient Pluripotent Stem CellsStem Cell Reports29141234Total RNA was extracted from Dgcr8−/−-302 cells and submitted to the Genomic Services Lab at the HudsonAlpha Institute. The library was constructed by the standard miRNA library construction protocol (Illumina) and 15 million, 50 bp single-end readsFull Paper
2017Bowling, KM;Thompson, ML;Amaral, MD;Finnila, CR;Hiatt, SM;Engel, KL;Cochran, JN;Brothers, KB;East, KM;Gray, DE;Kelley, WV;Lamb, NE;Lose, EJ;Rich, CA;Simmons, S;Whittle, JS;Weaver, BT;Nesmith, AS;Myers, RM;Barsh, GS;Bebin, EM;Cooper, GM;Genomic diagnosis for children with intellectual disability and/or developmental delayGenome Med28554332Methods" (see Additional file 1). Whole-exome and whole-genome sequencing. Blood samples were sent for sequencing at the HudsonAlpha Genomic Services Laboratory (http://gsl.hudsonalpha.org). Genomic DNA was isolatedFull Paper
2017Harden, ME;Prasad, N;Griffiths, A;Munger, K;Modulation of microRNA-mRNA Target Pairs by Human Papillomavirus 16 OncoproteinsMBio28049151;... million 50-bp PE reads) was performed with the Illumina MiSeq Sequencing System (Illumina). miR-seq data analysis. Postprocessing of the miR-seq reads from each sample was performed according to the HudsonAlpha Genomic Services Laboratory (GSL)Full Paper
2017Weiss, G;Schlegel, A;Kottwitz, D;König, T;Tetzner, R;Validation of the SHOX2/PTGER4 DNA Methylation Marker Panel for Plasma-Based Discrimination between Patients with Malignant and Nonmalignant Lung DiseaseJ Thorac Oncol27544059The patient samples were either material collected over the course of several months by commercial partners (ProteoGenex Inc., Sofia Bio LLC) or leftover material from previously conducted clinical trials in colon cancer.21, 22 More specifically, patFull Paper
2017Steffen, MM;Davis, TW;McKay, RML;Bullerjahn, GS;Krausfeldt, LE;Stough, JMA;Neitzey, ML;Gilbert, NE;Boyer, GL;Johengen, TH;Gossiaux, DC;Burtner, AM;Palladino, D;Rowe, MD;Dick, GJ;Meyer, KA;Levy, S;Boone, BE;Stumpf, RP;Wynne, TT;Zimba, PV;Gutierrez, D;Wilhelm, SW;Ecophysiological Examination of the Lake Erie Microcystis Bloom in 2014: Linkages between Biology and the Water Supply Shutdown of Toledo, OHEnviron. Sci. Technol.28535339RNA was stored at −80 °C until sent to HudsonAlpha Institute for Biotechnology (Huntsville, AL) for sequencing. Total RNA concentrations and quality were assessed fluorometrically via RiboGreen (Life Technologies, Carlsbad, CA) followed by integrityFull Paper
2017Park, KC;Lee, M;Jeon, Y;Jeon, R;Baek, SH;Lee, H;Kim, KI;Skin-Specific Deletion of Mis18α Impedes Proliferation and Stratification of Epidermal KeratinocytesJ. Invest. Dermatol.27670610Materials and Methods. In situ hybridization. All procedures of in situ hybridization, except probe design and cloning, were performed by Phylogeny Inc. (Columbus, OH). Briefly, the whole bodies of C57BL/6 mice were frozen and cut into 10-μm sectionsFull Paper
2017Blanchet, P;Bebin, M;Bruet, S;Cooper, GM;Thompson, ML;Duban-Bedu, B;Gerard, B;Piton, A;Suckno, S;Deshpande, C;Clowes, V;Vogt, J;Turnpenny, P;Williamson, MP;Alembik, Y;, ;, ;Glasgow, E;McNeill, A;MYT1L mutations cause intellectual disability and variable obesity by dysregulating gene expression and development of the neuroendocrine hypothalamusPLoS Genet.28859103Département de Génétique Médicale, Hôpital Arnaud de Villeneuve, Montpellier Cedex 5, France.++Department of Neurology, University of Alabama at Birmingham, Birmingham, AL, United States of America.++Service de génétique médicale, Hôpitaux UniversitaFull Paper
2017Mojiri, A;Stoletov, K;Carrillo, MA;Willetts, L;Jain, S;Godbout, R;Jurasz, P;Sergi, CM;Eisenstat, DD;Lewis, JD;Jahroudi, N;Functional assessment of von Willebrand factor expression by cancer cells of non-endothelial originOncotarget28035064Immunofluorescent staining of cultured cells were performed as previously described [34]. Immunofluorescent staining of slides containing sectioned paraffin imbedded biopsies of normal human brain and glioma (Dr. D. Eisenstat from the Brain Tumor TisFull Paper
2017McDaniel, JM;Varley, KE;Gertz, J;Savic, DS;Roberts, BS;Bailey, SK;Shevde, LA;Ramaker, RC;Lasseigne, BN;Kirby, MK;Newberry, KM;Partridge, EC;Jones, AL;Boone, B;Levy, SE;Oliver, PG;Sexton, KC;Grizzle, WE;Forero, A;Buchsbaum, DJ;Cooper, SJ;Myers, RM;Genomic regulation of invasion by STAT3 in triple negative breast cancerOncotarget28030809... ell viability and invasion assays. All ChIP-seq and RNA-seq datasets have been deposited in GEO (accession number: GSE85579). SUPPLEMENTARY MATERIALS FIGURES AND TABLES We thank the members of the HudsonAlpha Genomic Services Laboratory for prFull Paper
2017Ranganathan, P;Ngankeu, A;Zitzer, NC;Leoncini, P;Yu, X;Casadei, L;Challagundla, K;Reichenbach, DK;Garman, S;Ruppert, AS;Volinia, S;Hofstetter, J;Efebera, YA;Devine, SM;Blazar, BR;Fabbri, M;Garzon, R;Serum miR-29a Is Upregulated in Acute Graft-versus-Host Disease and Activates Dendritic Cells through TLR BindingJ. Immunol.28159900... Go to: ACKNOWLEDGMENTS We thank Carlo M Croce at The Ohio State University for providing us with miR29a KO mice. We thank Jordan Smith for assisting in collection and processing of blood samples. Phylogeny Inc., Columbus, Ohio, performed LNA-Full Paper
2017Xu-Monette, ZY;Zhang, M;Li, J;Young, KH;PD-1/PD-L1 Blockade: Have We Found the Key to Unleash the Antitumor Immune Response?Front Immunol2925545899.5%; 75.6% of patients had a response for ≥6 months Clinical activity was seen across all PD-L1 groups defined by PD-L1 intensity score, tumor-membrane staining score, and histiocyte score (QualTek IHC assay); 90.4% of patients had an intensityFull Paper
2017Zeng, Q;Tian, X;Wang, L;Genetic adaptation of microbial populations present in high-intensity catfish production systems with therapeutic oxytetracycline treatmentSci Rep29235508;... DNA (~1000 ng/sample) extracted from every sediment sample collected from the treated or the control system, were pooled for sequencing. The library construction and sequencing were conducted at the HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2017Oliveira, ERA;Póvoa, TF;Nuovo, GJ;Allonso, D;Salomão, NG;Basílio-de-Oliveira, CA;Geraldo, LHM;Fonseca, CG;Lima, FRS;Mohana-Borges, R;Paes, MV;Dengue fatal cases present virus-specific HMGB1 response in peripheral organsSci Rep29167501... iro, Brazil 2Instituto de Criminalística, Tocantins, Brazil 30000 0001 2285 7943grid.261331.4Ohio State University Comprehensive Cancer Center, Columbus, Ohio, United States of America 4grid.430298.4Phylogeny Inc, Powell, Ohio, United States of AFull Paper
2017Zhang, Y;Saavedra, E;Tang, R;Gu, Y;Lappin, P;Trajkovic, D;Liu, SH;Smeal, T;Fantin, V;De Botton, S;Legrand, O;Delhommeau, F;Pernasetti, F;Louache, F;Targeting primary acute myeloid leukemia with a new CXCR4 antagonist IgG1 antibody (PF-06747143)Sci Rep28779088... ratio and 1:50 ratios, respectively (n = 4/group). ToxiLight bioluminescent cytotoxicity assay (Lonza) was used to detect cell lysis. The AML patient primary bone marrow sample was obtained from Conversant Bio (sample ID #110032873). Mice NSG mFull Paper
2017Aumiller, V;Strobel, B;Romeike, M;Schuler, M;Stierstorfer, BE;Kreuz, S;Comparative analysis of lysyl oxidase (like) family members in pulmonary fibrosisSci Rep28273952Normal and fibrotic lung tissue samples from the BLM-induced mouse model were prepared and Masson trichrome stained as described previously27. Human IPF and healthy lung tissue was purchased at Folio bioscience (Columbus, Ohio, USA) following the guiFull Paper
2017Jane, EP;Premkumar, DR;Sutera, PA;Cavaleri, JM;Pollack, IF;Survivin inhibitor YM155 induces mitochondrial dysfunction, autophagy, DNA damage and apoptosis in Bcl-xL silenced glioma cell linesMol. Carcinog.27805285collected at the time of tumor resection. We also obtained primary GBM cells from Conversant Biologics (Huntsville, AL), which were cultured in DMEM/F12 (Invitrogen, Carlsbad, CA). GIBCO® Human Astrocytes, normal human cellsFull Paper
2017Appleby, TC;Greenstein, AE;Hung, M;Liclican, A;Velasquez, M;Villaseñor, AG;Wang, R;Wong, MH;Liu, X;Papalia, GA;Schultz, BE;Sakowicz, R;Smith, V;Kwon, HJ;Biochemical characterization and structure determination of a potent, selective antibody inhibitor of human MMP9J. Biol. Chem.28235803Ulcerative colitis tissue was obtained after surgical colectomy (Folio Biosciences). Specimens were selected based on disease severity scored by a pathologist on matched FFPE tissues. Frozen fragments of ~50 mg were lysed in 10 mM Hepes pH 7.5, 150 mFull Paper
2017Cho, JH;Sorensen, SF;Choi, YL;Feng, Y;Kim, TE;Choi, H;Georgsen, JB;Dolled-Filhart, M;Emancipator, K;Meldgaard, P;Sun, JM;Kim, HK;Choi, YS;Shim, YM;Zhou, W;Hager, H;Kim, J;Programmed Death Ligand 1 Expression in Paired Non-Small Cell Lung Cancer Tumor SamplesClin Lung Cancer28669849charged slides. The TechMate 500 instrument (QualTek, Goleta, CA) was used with the 22C3 antibody for automated staining. A membrane staining. All of the PD-L1 IHC assays were done at QualTek, as the central laboratory. PDFull Paper
2017Sundahl, N;De Wolf, K;Rottey, S;Decaestecker, K;De Maeseneer, D;Meireson, A;Goetghebeur, E;Fonteyne, V;Verbeke, S;De Visschere, P;Reynders, D;Van Gele, M;Brochez, L;Ost, P;A phase I/II trial of fixed-dose stereotactic body radiotherapy with sequential or concurrent pembrolizumab in metastatic urothelial carcinoma: evaluation of safety and clinical and immunologic responseJ Transl Med28662677Exploratory endpoints. Response rate according to expression of PD-L1. PD-L1 expression will be determined by immunohistochemistry in QualTek Molecular Laboratories on an archival or newly obtained tissue sample of the tumor or metastatic lesionFull Paper
2017Lagatie, O;Van Dorst, B;Stuyver, LJ;Identification of three immunodominant motifs with atypical isotype profile scattered over the Onchocerca volvulus proteomePLoS Negl Trop Dis28125577For determination of specificity of the peptide ELISA’s, different sets of plasma samples were obtained from Discovery Life Sciences, Inc. (Los Osos, USA), Bioreclamation IVT (Baltimore, USA), FR3 or Mayo Clinic (Jacksonville, USA). Written informedFull Paper
2017Pellegrini, KL;Patil, D;Douglas, KJS;Lee, G;Wehrmeyer, K;Torlak, M;Clark, J;Cooper, CS;Moreno, CS;Sanda, MG;Detection of prostate cancer-specific transcripts in extracellular vesicles isolated from post-DRE urineProstate28419548; targeted in this analysis). The libraries were sequenced at the HudsonAlpha Genomic Services Lab to produce 150 bases of paired‐end reads with an average of over 300 000 reads mapped per sample. The 150 base sequenceFull Paper
2017Wilson, CM;Klingeman, DM;Schlachter, C;Syed, MH;Wu, CW;Guss, AM;Brown, SD;LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313Appl. Environ. Microbiol.28003194;... Samples were pooled into three groups to make a stock of 20 nM in 72 μl. A 40-μl aliquot of the three pooled libraries was sequenced using an SR50 sequencing run on an Illumina HiSeq 2500 platform (HudsonAlpha Genomic Services Laboratory, HuntsvFull Paper
2017Zhang, Y;Song, J;Day, K;Absher, D;dCATCH-Seq: improved sequencing of large continuous genomic targets with double-hybridizationBMC Genomics29061109HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA.++HudsonAlpha Institute for Biotechnology, Huntsville, USA. dabsher@hudsonaFull Paper
2017Matsunaga, Y;Hwang, H;Franke, B;Williams, R;Penley, M;Qadota, H;Yi, H;Morran, LT;Lu, H;Mayans, O;Benian, GM;Twitchin kinase inhibits muscle activityMol. Biol. Cell28428253Genomic DNA from unc-22(sf21) was prepared and provided to the Emory Integrated Genomics Core (EIGC) for Illumina sequencing. The Genomic Services Laboratory at the HudsonAlpha Institute for Biotechnology performed quality control and constructed staFull Paper
2017Liu, Z;Zhang, C;Khodadadi-Jamayran, A;Dang, L;Han, X;Kim, K;Li, H;Zhao, R;Canonical microRNAs Enable Differentiation, Protect Against DNA Damage, and Promote Cholesterol Biosynthesis in Neural Stem CellsStem Cells Dev.27762676For mRNA-seq and small RNA-seq analyses, total RNA samples prepared from Dgcr8+/− and Dgcr8−/− NSCs were submitted to the Genomic Services Laboratory at HudsonAlpha Institute (Huntsville, AL) for library construction and sequencing. For mRNA-seq analFull Paper
2017Aycock, KI;Campbell, RL;Lynch, FC;Manning, KB;Craven, BA;Computational predictions of the embolus-trapping performance of an IVC filter in patient-specific and idealized IVC geometriesBiomech Model Mechanobiol28656515Full Paper
2017George, AF;Rahman, KM;Camp, ME;Prasad, N;Bartol, FF;Bagnell, CA;Defining age- and lactocrine-sensitive elements of the neonatal porcine uterine microRNA-mRNA interactomeBiol. Reprod.28203709Department of Animal Sciences, Endocrinology, and Animal Biosciences Program, Rutgers University, New Brunswick, New Jersey, USA.++Department of Animal Sciences, Endocrinology, and Animal Biosciences Program, Rutgers University, New Brunswick, New JeFull Paper
2017Kirby, MK;Ramaker, RC;Roberts, BS;Lasseigne, BN;Gunther, DS;Burwell, TC;Davis, NS;Gulzar, ZG;Absher, DM;Cooper, SJ;Brooks, JD;Myers, RM;Genome-wide DNA methylation measurements in prostate tissues uncovers novel prostate cancer diagnostic biomarkers and transcription factor binding patternsBMC Cancer28412973;... ng characteristic RRBS Reduced representation bisulfite sequencing TCGA The cancer genome atlas TF Transcription factor TMPRSS2 Transmembrane protease, serine 2 Acknowledgements We thank the HudsonAlpha Genomic Services Lab for providinFull Paper
2017Costa-Paiva, EM;Whelan, NV;Waits, DS;Santos, SR;Schrago, CG;Halanych, KM;Discovery and evolution of novel hemerythrin genes in annelid wormsBMC Evol. Biol.28330441Despite extensive study on hemoglobins and hemocyanins, little is known about hemerythrin (Hr) evolutionary history. Four subgroups of Hrs have been documented, including: circulating Hr (cHr), myohemerythrin (myoHr), ovohemerythrin (ovoHr), and neurFull Paper
2017Pizzi, M;Inghirami, G;Patient-derived tumor xenografts of lymphoproliferative disorders: are they surrogates for the human disease?Curr. Opin. Hematol.28594662Patient-derived xenograft models WuXi AppTec http://www.urolead.com Xenografts Conversant Bio http://www.conversantbio.com/pricing Xenograft models ONCOdesign http://www.oncodesign. com/en/contact-us Humanized mice HuMurine http://humurine.comFull Paper
2017Zhang, D;Thongda, W;Li, C;Zhao, H;Beck, BH;Mohammed, H;Arias, CR;Peatman, E;More than just antibodies: Protective mechanisms of a mucosal vaccine against fish pathogen Flavobacterium columnareFish Shellfish Immunol.28989091; 2000 instrument (Thermo Scientific, Wilmington, DE). RNA-seq library preparation and sequencing were carried out by the HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). Briefly, TruSeq RNA Sample PreparationFull Paper
2017Zhu, J;Fu, Q;Ao, Q;Tan, Y;Luo, Y;Jiang, H;Li, C;Gan, X;Transcriptomic profiling analysis of tilapia (Oreochromis niloticus) following Streptococcus agalactiae challengeFish Shellfish Immunol.28111359; For each timepoint, equal amount of RNA from individual fish were pooled together (15 fish) for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out in HudsonAlpha Genomic Services Lab (Huntsville, AL, USA)Full Paper
2017McCoy, CR;Jackson, NL;Day, J;Clinton, SM;Genetic predisposition to high anxiety- and depression-like behavior coincides with diminished DNA methylation in the adult rat amygdalaBehav. Brain Res.27965039; Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) for next-generation sequencing using NEBNext reagents (New England Biolabs, Ipswich, MA) according to manufacturer's recommendationsFull Paper
2017Stebbins, KJ;Broadhead, AR;Cabrera, G;Correa, LD;Messmer, D;Bundey, R;Baccei, C;Bravo, Y;Chen, A;Stock, NS;Prasit, P;Lorrain, DS;In vitro and in vivo pharmacology of NXT629, a novel and selective PPARα antagonistEur. J. Pharmacol.28483457expression of PPARα, normal healthy or melanoma skin biopsy samples (n=2 donors each) were obtained from ConversantBio (Hunstville, Alabama) Normal (n=5) and melanoma (n=4) human skin biopsy samples were obtained from Conversant Bio and expressionFull Paper
2017Pirie-Shepherd, SR;Painter, C;Whalen, P;Vizcarra, P;Roy, M;Qian, J;Franks, T;Coskran, T;Golas, J;Deng, S;Zhong, W;Tucker, E;Marrinucci, D;Gerber, HP;Powell, EL;Detecting expression of 5T4 in CTCs and tumor samples from NSCLC patientsPLoS ONE28727782... d from a variety of sources, including the Pfizer tumor bank and commercial vendors. All commercially sourced human biospecimens, including blood samples and matched tumor tissues, were obtained from Conversant Bio. Biospecimens distributed by CoFull Paper
2017Awasthi Mishra, N;Drögemüller, C;Jagannathan, V;Keller, I;Wüthrich, D;Bruggmann, R;Beck, J;Schütz, E;Brenig, B;Demmel, S;Moser, S;Signer-Hasler, H;Pieńkowska-Schelling, A;Schelling, C;Sande, M;Rongen, R;Rieder, S;Kelsh, RN;Mercader, N;Leeb, T;A structural variant in the 5'-flanking region of the TWIST2 gene affects melanocyte development in belted cattlePLoS ONE28658273Center Zurich, and the Genomic Services Lab at HudsonAlpha for performing experiments. Computationally intensive tasks were performed at the the Interfaculty Bioinformatics Unit of the University of Bern and the Vital-IT high-performance computing ceFull Paper
2017Carlson, DE;Hedin, M;Comparative transcriptomics of Entelegyne spiders (Araneae, Entelegynae), with emphasis on molecular evolution of orphan genesPLoS ONE28379977Next-generation sequencing technology is rapidly transforming the landscape of evolutionary biology, and has become a cost-effective and efficient means of collecting exome information for non-model organisms. Due to their taxonomic diversity, producFull Paper
2017Howard, LM;Hoek, KL;Goll, JB;Samir, P;Galassie, A;Allos, TM;Niu, X;Gordy, LE;Creech, CB;Prasad, N;Jensen, TL;Hill, H;Levy, SE;Joyce, S;Link, AJ;Edwards, KM;Cell-Based Systems Biology Analysis of Human AS03-Adjuvanted H5N1 Avian Influenza Vaccine Responses: A Phase I Randomized Controlled TrialPLoS ONE28099485Vanderbilt Vaccine Research Program, Department of Pediatrics, Vanderbilt University School of Medicine, Nashville, TN, United States of America.++Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, NashvilFull Paper
2017O'Neil, BH;Wallmark, JM;Lorente, D;Elez, E;Raimbourg, J;Gomez-Roca, C;Ejadi, S;Piha-Paul, SA;Stein, MN;Abdul Razak, AR;Dotti, K;Santoro, A;Cohen, RB;Gould, M;Saraf, S;Stein, K;Han, SW;Safety and antitumor activity of the anti-PD-1 antibody pembrolizumab in patients with advanced colorectal carcinomaPLoS ONE29284010mbedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory-developed prototype immunohistochemistry (IHC) assay (QualTek Molecular Laboratories, Goleta, CA, USA) [Full Paper
2017Danaee, H;Kalebic, T;Wyant, T;Fassan, M;Mescoli, C;Gao, F;Trepicchio, WL;Rugge, M;Consistent expression of guanylyl cyclase-C in primary and metastatic gastrointestinal cancersPLoS ONE29261789GCC IHC analysis and pathology assessment. GCC staining was performed on the Techmate Automated IHC Platform (BioTek Solutions/Ventana Medical Systems, Tucson, AZ, USA; QualTek Laboratories, Goleta, CA, USA) ;two independent pathologists. GCC IFull Paper
2017O'Neil, BH;Wallmark, JM;Lorente, D;Elez, E;Safety and antitumor activity of the anti–PD-1 antibody pembrolizumab in patients with advanced colorectal carcinomaPloS onearchived formalin-fixed, paraffin-embedded tumor sample or a newly obtained biopsy specimen was assessed at a central laboratory for PD-L1 expression at screening with a laboratory- developed prototype immunohistochemistry (IHC) assay (QualTek MolecuFull Paper
2017Eboigbodin, KE;Moilanen, K;Elf, S;Hoser, M;Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA®BMC Infect. Dis.28183291isothermal conditions. Methods. Clinical specimens and rapid processing. A total of 40 nasopharyngeal (NP) swab specimens were obtained from Discovery Life Sciences Biobank (Discovery Life Sciences, Inc., CA, USA). TheFull Paper
2017Liu, DT;Brewer, MS;Chen, S;Hong, W;Zhu, Y;Transcriptomic signatures for ovulation in vertebratesGen. Comp. Endocrinol.28111234PCR (qPCR) assay. 2.4. Library construction and Illumina sequencing. RNA-Seq library preparation and high-throughput NGS sequencing were carried out at HudsonAlpha Genomic Services Lab (Huntsville, AL). Qubit® 3.0Full Paper
2017Bhayana, S;Song, F;Jacob, J;Fadda, P;Denko, NC;Xu-Welliver, M;Chakravarti, A;Jacob, NK;Urinary miRNAs as Biomarkers for Noninvasive Evaluation of Radiation-Induced Renal Tubular InjuryRadiat. Res.28977780W81XWH-15-2-0054). We thank Dr. Jerry Nuovo of Phylogeny, Inc. (Powell, OH) for technical assistance. REFERENCES. 1. Hingorani S. Chronic kidney disease in long-term survivors of hematopoietic cell transplantation: epidemiology, pathogenesis, and treFull Paper
2017Kadić, E;Moniz, RJ;Huo, Y;Chi, A;Kariv, I;Effect of cryopreservation on delineation of immune cell subpopulations in tumor specimens as determinated by multiparametric single cell mass cytometry analysisBMC Immunol.28148223... ry detection antibodies Sample sourcing PBMCs were purchased from SeraCare Life Sciences Inc., (Milford, MA). Frozen dissociated tumor cells (DTC) and normal adjacent tissue (NAT) were purchased from ConversantBio (Huntsville, AL). The frozen samFull Paper
2017Parlato, M;Molenda, J;Murphy, WL;Specific recruitment of circulating angiogenic cells using biomaterials as filtersActa biomaterialia28373084CD133+ cells collected from mobilized peripheral blood were purchased from AllCells, LLC. These cells were collected by AllCells, LLC from one donor (26 year old, African American, SaveFull Paper
2017Block, TS;Murphy, TI;Munster, PN;Nguyen, DP;Lynch, FJ;Glucocorticoid receptor expression in 20 solid tumor types using immunohistochemistry assayCancer Manag Res28293120Tiffany I Murphy. 3 QualTek Molecular Laboratories, Newtown, PA. Find articles by Tiffany I Murphy. Pamela N Munster Dat P Nguyen. 1 Corcept Therapeutics, Inc, Menlo Park, CA. Find articles by Dat P Nguyen. Frank J Lynch. 3 QualTek Molecular LaboraFull Paper
2017Coletti, M;Hultquist, C;Kennedy, WG;Cervone, G;Validating Safecast data by comparisons to a U. S. Department of Energy Fukushima Prefecture aerial surveyJ Environ Radioact28167372Second, units are randomly selected, assembled, and undergo calibration tests at the Julich Research Centre in Germany, QualTek in the US, and the International Atomic Energy Agency (IAEA) testing laboratory in Seibersdorf, AustriaFull Paper
2017Thabet, AA;Maas, AE;Saber, SA;Tarrant, AM;Assembly of a reference transcriptome for the gymnosome pteropod Clione limacina and profiling responses to short-term CO2 exposureMar Genomics28385518Total RNA was extracted from pools of C. limacina juveniles using the Aurum Total RNA Fatty and Fibrous Tissue Kit (Bio-Rad). RNA purity and integrity were quantified using a Nanodrop ND-1000 spectrophotometer, and quality was assessed using a BioanaFull Paper
2017Matsushita, M;Ozawa, K;Suzuki, T;Nakamura, M;Nakano, N;Kanchi, S;Ichikawa, D;Matsuki, E;Sakurai, M;Karigane, D;Kasahara, H;Tsukamoto, N;Shimizu, T;Mori, T;Nakajima, H;Okamoto, S;Kawakami, Y;Hattori, Y;CXorf48 is a potential therapeutic target for achieving treatment-free remission in CML patientsBlood cancer journal28862699ty gradient centrifugation and cryopreserved in Cell Banker reagent (Juji-Field, Tokyo, Japan) in liquid nitrogen until further use. Bone marrow mononuclear cells (BMMNC) from HDs were purchased from AllCells, LLC. (Alameda, CA, USA). This study wasFull Paper
2017Simeonov, DR;Gowen, BG;Boontanrart, M;Roth, TL;Gagnon, JD;Mumbach, MR;Satpathy, AT;Lee, Y;Bray, NL;Chan, AY;Lituiev, DS;Nguyen, ML;Gate, RE;Subramaniam, M;Li, Z;Woo, JM;Mitros, T;Ray, GJ;Curie, GL;Naddaf, N;Chu, JS;Ma, H;Boyer, E;Van Gool, F;Huang, H;Liu, R;Tobin, VR;Schumann, K;Daly, MJ;Farh, KK;Ansel, KM;Ye, CJ;Greenleaf, WJ;Anderson, MS;Bluestone, JA;Chang, HY;Corn, JE;Marson, A;Discovery of stimulation-responsive immune enhancers with CRISPR activationNature28854172Normal donor human peripheral blood cells were obtained fresh from AllCells. CD4 + T cells were enriched from peripheral blood using the RosetteSep Human CD4 + T Cell SaveFull Paper
2017Kawamura, S;Onai, N;Miya, F;Sato, T;Tsunoda, T;Kurabayashi, K;Yotsumoto, S;Kuroda, S;Takenaka, K;Akashi, K;Ohteki, T;Identification of a Human Clonogenic Progenitor with Strict Monocyte Differentiation Potential: A Counterpart of Mouse cMoPsImmunity28514689Human UCB samples were provided by the Japanese Red Cross Kanto-Koshinetsu Cord Blood Bank, and human BM samples were purchased from AllCells. All experiments were SaveFull Paper
2017Peterson, VM;Zhang, KX;Kumar, N;Wong, J;Li, L;Wilson, DC;Moore, R;McClanahan, TK;Sadekova, S;Klappenbach, JA;Multiplexed quantification of proteins and transcripts in single cellsNature biotechnology28854175Cryopreserved PBMCs from a healthy donor (AllCells, PB003F, LotA5051) were thawed at 37 C for 3 min and resuspended in 10 ml of PBS with 1% Bovine Serum Albumin (BSA) ( SaveFull Paper
2017Zheng, C;Zheng, L;Yoo, JK;Guo, H;Zhang, Y;Guo, X;Kang, B;Hu, R;Huang, JY;Zhang, Q;Liu, Z;Dong, M;Hu, X;Ouyang, W;Peng, J;Zhang, Z;Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell SequencingCell28622514Anti-FOXP3 antibody (IHC) Abcam Cat#ab22510 CD3 Functional Grade Monoclonal Antibody eBioscience Cat#16-0037-85 CD28 Functional Grade Monoclonal Antibody eBioscience Cat#16-0289-85 Biological Samples Human PBMC AllCells https://www.allcells.comFull Paper
2017Sinha, G;The organoid architectScience (New York, N.Y.)28839056After almost a decade of tedious experiments, Clevers's postdoc Nick Barker struckgold in 2007: He discovered that cells carrying a receptor named LGR5 give rise to allcells in mouse SaveFull Paper
2017Stoeckius, M;Hafemeister, C;Stephenson, W;Houck-Loomis, B;Chattopadhyay, PK;Swerdlow, H;Satija, R;Smibert, P;Simultaneous epitope and transcriptome measurement in single cellsNature methods28759029ononuclear cells (CBMCs) were isolated from cord blood (New York Blood Center) as described25. Cells were kept on ice during and after isolation. Peripheral blood mononuclear cells were obtained from Allcells (USA). Comparing flow cytometry and CITEFull Paper
2017Seet, CS;He, C;Bethune, MT;Li, S;Chick, B;Gschweng, EH;Zhu, Y;Kim, K;Kohn, DB;Baltimore, D;Crooks, GM;Montel-Hagen, A;Generation of mature T cells from human hematopoietic stem and progenitor cells in artificial thymic organoidsNature methods28369043placental units from deliveries at UCLA. Bone marrow (BM) was obtained from healthy adult donors (ages 18-51) through discarded material from allogeneic BM donor harvests at UCLA or purchased from AllCells Inc. (Alameda, CA). G-CSF mobilized peripherFull Paper
2017Mumbach, MR;Satpathy, AT;Boyle, EA;Dai, C;Gowen, BG;Cho, SW;Nguyen, ML;Rubin, AJ;Granja, JM;Kazane, KR;Wei, Y;Nguyen, T;Greenside, PG;Corces, MR;Tycko, J;Simeonov, DR;Suliman, N;Li, R;Xu, J;Flynn, RA;Kundaje, A;Khavari, PA;Marson, A;Corn, JE;Quertermous, T;Greenleaf, WJ;Chang, HY;Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elementsNature genetics28945252Normal donor human peripheral blood cells were obtained fresh from AllCells. CD4 + T cells were enriched from peripheral blood using RosetteSep Human CD4 + T Cell Enrichment SaveFull Paper
2017Hao, H;Hu, S;Chen, H;Bu, D;Zhu, L;Xu, C;Chu, F;Huo, X;Tang, Y;Sun, X;Ding, BS;Liu, DP;Hu, S;Wang, M;Loss of Endothelial CXCR7 Impairs Vascular Homeostasis and Cardiac Remodeling After Myocardial Infarction: Implications for Cardiovascular Drug DiscoveryCirculation28154007Human umbilical vein endothelial cells were obtained from AllCells. The CXCL12 concentration was determined with a human CXCL12 ELISA kit (R&D Systems). Calcium release was SaveFull Paper
2017Gong, X;Litchfield, LM;Webster, Y;Chio, LC;Wong, SS;Stewart, TR;Dowless, M;Dempsey, J;Zeng, Y;Torres, R;Boehnke, K;Mur, C;Marugán, C;Baquero, C;Yu, C;Bray, SM;Wulur, IH;Bi, C;Chu, S;Qian, HR;Iversen, PW;Merzoug, FF;Ye, XS;Reinhard, C;De Dios, A;Du, J;Caldwell, CW;Lallena, MJ;Beckmann, RP;Buchanan, SG;Genomic Aberrations that Activate D-type Cyclins Are Associated with Enhanced Sensitivity to the CDK4 and CDK6 Inhibitor AbemaciclibCancer cell29232554All cell lines were obtained from commercial vendors and were cultured in conditions recommended by vendors (Table S8; AllCells: https://www.allcells.com/; ATCC: https://www.atcc.org/; CASTCC: http://www.cellbank.org.cn; Chempartner:Full Paper
2017McKeown, MR;Corces, MR;Eaton, ML;Fiore, C;Lee, E;Lopez, JT;Chen, MW;Smith, D;Chan, SM;Koenig, JL;Austgen, K;Guenther, MG;Orlando, DA;Lovén, J;Fritz, CC;Majeti, R;Superenhancer Analysis Defines Novel Epigenomic Subtypes of Non-APL AML, Including an RARα Dependency Targetable by SY-1425, a Potent and Selective RARα AgonistCancer discovery28729405Normal donor human blood cells were obtained fresh from AllCells (Alameda, CA) and FACS-enriched. Human AML samples were obtained from patients at the Stanford Medical Center with informed consent, according to Institutional Review Board (IRB)-approvFull Paper
2017Glass, JL;Hassane, D;Wouters, BJ;Kunimoto, H;Avellino, R;Garrett-Bakelman, FE;Guryanova, OA;Bowman, R;Redlich, S;Intlekofer, AM;Meydan, C;Qin, T;Fall, M;Alonso, A;Guzman, ML;Valk, PJM;Thompson, CB;Levine, R;Elemento, O;Delwel, R;Melnick, A;Figueroa, ME;Epigenetic Identity in AML Depends on Disruption of Nonpromoter Regulatory Elements and Is Affected by Antagonistic Effects of Mutations in Epigenetic ModifiersCancer discovery28408400based on sample availability. Twenty-two normal CD34+ bone marrow specimens were obtained from AllCells (Alameda, CA). In addition, 6 AML samples were obtained from the Memorial Sloan Kettering Hematologic Oncology Tissue Bank for colony forming assaFull Paper
2017Pandya, H;Shen, MJ;Ichikawa, DM;Sedlock, AB;Choi, Y;Johnson, KR;Kim, G;Brown, MA;Elkahloun, AG;Maric, D;Sweeney, CL;Gossa, S;Malech, HL;McGavern, DB;Park, JK;Differentiation of human and murine induced pluripotent stem cells to microglia-like cellsNature neuroscience28253233RNA from human dendritic and bone marrow macrophages (AllCells) was also used. Isolated total RNA was amplified with the WT Expression Kit (Ambion) and labeled with the SaveFull Paper
2017Rozo, C;Chinenov, Y;Maharaj, RK;Gupta, S;Leuenberger, L;Kirou, KA;Bykerk, VP;Goodman, SM;Salmon, JE;Pernis, AB;Targeting the RhoA-ROCK pathway to reverse T-cell dysfunction in SLEAnnals of the rheumatic diseases28283529In separate experiments, cryopreserved human cord blood CD4 + T lymphocytes from different donors were obtained from Allcells, LLC. CD4 + T-cell purity was >95%. Cells were SaveFull Paper
2017Miyawaki, K;Iwasaki, H;Jiromaru, T;Kusumoto, H;Yurino, A;Sugio, T;Uehara, Y;Odawara, J;Daitoku, S;Kunisaki, Y;Mori, Y;Arinobu, Y;Tsuzuki, H;Kikushige, Y;Iino, T;Kato, K;Takenaka, K;Miyamoto, T;Maeda, T;Akashi, K;Identification of unipotent megakaryocyte progenitors in human hematopoiesisBlood28336526Human adult bone marrow (BM) samples were obtained from healthy donors or purchased from AllCells. BM samples of adult hematological malignancies, diagnosed according to SaveFull Paper
2017Leong, SR;Sukumaran, S;Hristopoulos, M;Totpal, K;Stainton, S;Lu, E;Wong, A;Tam, L;Newman, R;Vuillemenot, BR;Ellerman, D;Gu, C;Mathieu, M;Dennis, MS;Nguyen, A;Zheng, B;Zhang, C;Lee, G;Chu, YW;Prell, RA;Lin, K;Laing, ST;Polson, AG;An anti-CD3/anti-CLL-1 bispecific antibody for the treatment of acute myeloid leukemiaBlood27908880(CD) CD8+ T cells were purified from peripheral blood of a healthy human donor (ALLCells, Alameda, CA) for donor 1 and a Genentech donor for donor 2 by Ficoll density gradient SaveFull Paper
2017Weiss, M;Genome Editing Strategies to Treat Beta-HemoglobinopathiesBloodFrozen CD34+ cells from mobilized peripheral blood of normal individuals were purchased from AllCells. Peripheral blood CD34+ cells were acquired from SCD patients undergoing therapeutic red cell exchange at the Cancer & Hematology Centers, Texas ChiFull Paper
2017Tisdale, J;Pierciey, F;Kamble, R;Kanter, J;Krishnamurti, L;Kwiatkowski, J;Thompson, A;Shestopalov, I;Bonner, M;Joseney-Antoine, M;Asmal, M;Walters, M;Successful Plerixafor-Mediated Mobilization, Apheresis, and Lentiviral Vector Transduction of Hematopoietic Stem Cells in Patients with Severe Sickle Cell DiseaseBloodBackground Patients with severe sickle cell disease (SCD) may benefit from β-globin gene transfer into autologous hematopoietic stem cells (HSC). Successful HBB gene transfer requires vector-mediated transduction of primitive HSCs. SteFull Paper
2017Fujii, S;Miura, Y;Fujishiro, A;Shindo, T;Shimazu, Y;Human Bone Marrow Mesenchymal Stromal/Stem Cell-Derived Extracellular Vesicles Ameliorate Graft-Versus-Host Disease-Related ManifestationsBloodHuman MSC were isolated from BM samples purchased from AllCells (Alameda, CA) based on our previously published method (Biochem Biophys Res Commun 2016;469:823). EV SaveFull Paper
2017Mathiyalagan, P;Liang, Y;Kim, D;Misener, S;Thorne, T;Kamide, CE;Klyachko, E;Losordo, DW;Hajjar, RJ;Sahoo, S;Angiogenic Mechanisms of Human CD34+ Stem Cell Exosomes in the Repair of Ischemic HindlimbCirculation research28298297The cells were purified from mobilized peripheral blood mononuclear cells (AllCells LLC, Emeryville, CA) with an Isolex 300i device (Baxter Healthcare); cell purity (85% to 95%) was SaveFull Paper
2017Elagib, KE;Lu, CH;Mosoyan, G;Khalil, S;Zasadzińska, E;Foltz, DR;Balogh, P;Gru, AA;Fuchs, DA;Rimsza, LM;Verhoeyen, E;Sansó, M;Fisher, RP;Iancu-Rubin, C;Goldfarb, AN;Neonatal expression of RNA-binding protein IGF2BP3 regulates the human fetal-adult megakaryocyte transitionThe Journal of clinical investigation28481226The normal donor samples purchased from the Fred Hutchinson Cancer Research Center and AllCells were originally obtained with informed consent and IRB approval at both SaveFull Paper
2017Chung, SS;Eng, WS;Hu, W;Khalaj, M;Garrett-Bakelman, FE;Tavakkoli, M;Levine, RL;Carroll, M;Klimek, VM;Melnick, AM;Park, CY;CD99 is a therapeutic target on disease stem cells in myeloid malignanciesScience translational medicine28123069consent under IRB-approved protocols. Umbilical cord blood (CB) specimens were purchased from the New York Blood Center (New York, New York). Normal human BM specimens were purchased from AllCells Inc. (Emeryville, California)Full Paper
2017Zhang, T;Chi, S;Jiang, F;Zhao, Q;Xiao, B;A protein interaction mechanism for suppressing the mechanosensitive Piezo channelsNature communications29176668(MEM) containing 10% FBS, non-essential amino acids, 1 mM sodium pyruvate, 100 U ml−1 penicillin and 100 μg ml−1 streptomycin. Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured using EGM-2 groFull Paper
2017Trabanelli, S;Chevalier, MF;Martinez-Usatorre, A;Gomez-Cadena, A;Salomé, B;Lecciso, M;Salvestrini, V;Verdeil, G;Racle, J;Papayannidis, C;Morita, H;Pizzitola, I;Grandclément, C;Bohner, P;Bruni, E;Girotra, M;Pallavi, R;Falvo, P;Leibundgut, EO;Baerlocher, GM;Carlo-Stella, C;Taurino, D;Santoro, A;Spinelli, O;Rambaldi, A;Giarin, E;Basso, G;Tresoldi, C;Ciceri, F;Gfeller, D;Akdis, CA;Mazzarella, L;Minucci, S;Pelicci, PG;Marcenaro, E;McKenzie, ANJ;Vanhecke, D;Coukos, G;Mavilio, D;Curti, A;Derré, L;Jandus, C;Tumour-derived PGD2 and NKp30-B7H6 engagement drives an immunosuppressive ILC2-MDSC axisNature communications28928446Human CD34 + cells were purchased from either Lonza (CH) or AllCells (USA). NSG mice were purchased from The Jackson Laboratory (stock number: 005557) and bred, treated and SaveFull Paper
2017Zheng, GX;Terry, JM;Belgrader, P;Ryvkin, P;Bent, ZW;Wilson, R;Ziraldo, SB;Wheeler, TD;McDermott, GP;Zhu, J;Gregory, MT;Shuga, J;Montesclaros, L;Underwood, JG;Masquelier, DA;Nishimura, SY;Schnall-Levin, M;Wyatt, PW;Hindson, CM;Bharadwaj, R;Wong, A;Ness, KD;Beppu, LW;Deeg, HJ;McFarland, C;Loeb, KR;Valente, WJ;Ericson, NG;Stevens, EA;Radich, JP;Mikkelsen, TS;Hindson, BJ;Bielas, JH;Massively parallel digital transcriptional profiling of single cellsNature communications28091601s Jurkat (ATCC TIB-152), 293T (ATCC CRL-11268) and 3T3 (ATCC CRL-1658) cells were acquired from ATCC and cultured according to ATCC guidelines. Fresh PBMCs, frozen PBMCs and BMMCs were purchased from ALLCELLS. Frozen PBMCs from Donor A were made fromFull Paper
2017Nakata, R;Shimada, H;Fernandez, GE;Fanter, R;Fabbri, M;Malvar, J;Zimmermann, P;DeClerck, YA;Contribution of neuroblastoma-derived exosomes to the production of pro-tumorigenic signals by bone marrow mesenchymal stromal cellsJournal of extracellular vesicles28717423Human BM-derived MSCs were purchased from AllCells or isolated in our laboratory from the BM of patients with NB using a previously published protocol [10]. Cells were maintained SaveFull Paper
2017Burton, OT;Stranks, AJ;Tamayo, JM;Koleoglou, KJ;Schwartz, LB;Oettgen, HC;A humanized mouse model of anaphylactic peanut allergyThe Journal of allergy and clinical immunology27417025Cord blood-derived flow-sorted human CD34+ hematopoietic stem cells (HSCs) were purchased from AllCells (Alameda, Calif). Such cells are obtained under Institutional Review Board- or Human Subject Committee-approved protocols. Cells (5 × 104-105) werFull Paper
2017Bolisetty, M;Stitzel, M;Robson, P;CellView: Interactive exploration of high dimensional single cell RNA-seq databioRxivSingle cell RNA-seq - PBMCs were purchased from AllCells, thawed quickly at 37C and into DMEM supplemented with 10% FBS. Cells were quickly spun down at 400g, for 10min. SaveFull Paper
2017Li, S;Siriwon, N;Zhang, X;Yang, S;Jin, T;He, F;Kim, YJ;Mac, J;Lu, Z;Wang, S;Han, X;Wang, P;Enhanced Cancer Immunotherapy by Chimeric Antigen Receptor-Modified T Cells Engineered to Secrete Checkpoint InhibitorsClinical cancer research : an official journal of the American Association for Cancer Research28912137Chimeric antigen receptor (CAR) T-cell based therapy has shown promise as an immunotherapeutic modality for cancers. It has achieved successful responses in patients with certain SaveFull Paper
2017Dai, X;Yan, J;Fu, X;Pan, Q;Sun, D;Xu, Y;Wang, J;Nie, L;Tong, L;Shen, A;Zheng, M;Huang, M;Tan, M;Liu, H;Huang, X;Ding, J;Geng, M;Aspirin Inhibits Cancer Metastasis and Angiogenesis via Targeting HeparanaseClinical cancer research : an official journal of the American Association for Cancer Research28710312from the Institute of Biochemistry and Cell Biology (Chinese Academy of Sciences); primary human umbilical vascular endothelial cells (HUVECs) were purchased from AllCells. CHO- SaveFull Paper
2017Das, DS;Das, A;Ray, A;Song, Y;Samur, MK;Munshi, NC;Chauhan, D;Anderson, KC;Blockade of Deubiquitylating Enzyme USP1 Inhibits DNA Repair and Triggers Apoptosis in Multiple Myeloma CellsClinical cancer research : an official journal of the American Association for Cancer Research28270494Purpose: The ubiquitin proteasome pathway is a validated therapeutic target in multiple myeloma. Deubiquitylating enzyme USP1 participates in DNA damage response and cellular SaveFull Paper
2017Liu, L;Lam, CK;Long, V;Widjaja, L;Yang, Y;Li, H;Jin, L;Burke, S;Gorlatov, S;Brown, J;Alderson, R;Lewis, MD;Nordstrom, JL;Koenig, S;Moore, PA;Johnson, S;Bonvini, E;MGD011, A CD19 x CD3 Dual-Affinity Retargeting Bi-specific Molecule Incorporating Extended Circulating Half-life for the Treatment of B-Cell MalignanciesClinical cancer research : an official journal of the American Association for Cancer Research27663593Cryopreserved purified primary CLL patient samples were from AllCells, LLC. Heparinized whole blood from cynomolgus monkeys was from Worldwide Primates, Inc. SaveFull Paper
2017DeRyckere, D;Lee-Sherick, AB;Huey, MG;Hill, AA;Tyner, JW;Jacobsen, KM;Page, LS;Kirkpatrick, GG;Eryildiz, F;Montgomery, SA;Zhang, W;Wang, X;Frye, SV;Earp, HS;Graham, DK;UNC2025, a MERTK Small-Molecule Inhibitor, Is Therapeutically Effective Alone and in Combination with Methotrexate in Leukemia ModelsClinical cancer research : an official journal of the American Association for Cancer Research27649555Deidentified cord blood and normal bone marrow samples were obtained commercially from Clinimmune Labs and ALLCELLS, respectively. SaveFull Paper
2017Stoeckius, M;Hafemeister, C;Stephenson, W;Houck-Loomis, B;Chattopadhyay, P;Swerdlow, H;Satija, R;Smibert, P;Large-scale simultaneous measurement of epitopes and transcriptomes in single cellsbioRxivCord blood mononuclear cells (CBMCs) were isolated from cord blood (New York Blood Center) as described30. Cells were kept on ice during and after isolation. Peripheral blood mononuclear cells were obtained from Allcells (USA)Full Paper
2017Cichocki, F;Valamehr, B;Bjordahl, R;Zhang, B;Rezner, B;Rogers, P;Gaidarova, S;Moreno, S;Tuininga, K;Dougherty, P;McCullar, V;Howard, P;Sarhan, D;Taras, E;Schlums, H;Abbot, S;Shoemaker, D;Bryceson, YT;Blazar, BR;Wolchko, S;Cooley, S;Miller, JS;GSK3 Inhibition Drives Maturation of NK Cells and Enhances Their Antitumor ActivityCancer research28790065Peripheral blood mononuclear cells (PBMCs) from healthy CMV seropositive donors were obtained from Memorial Blood Bank (Minneapolis, MN), AllCells Inc. (Alameda, CA) and Key Biologics (Memphis, TN). All samples obtained with written consent and wereFull Paper
2017Jiang, H;Rivera-Molina, Y;Gomez-Manzano, C;Clise-Dwyer, K;Bover, L;Vence, LM;Yuan, Y;Lang, FF;Toniatti, C;Hossain, MB;Fueyo, J;Oncolytic Adenovirus and Tumor-Targeting Immune Modulatory Therapy Improve Autologous Cancer VaccinationCancer research28566332Oncolytic viruses selectively lyse tumor cells, disrupt immunosuppression within the tumor, and reactivate antitumor immunity, but they have yet to live up to their therapeutic potential. SaveFull Paper
2017Chen, Y;Shan, Y;Lu, M;DeSouza, N;Guo, Z;Hoffman, R;Liang, A;Li, S;Alox5 Blockade Eradicates JAK2V617F-Induced Polycythemia Vera in MiceCancer research27784744GE Healthcare) and low-density mononuclear cells were separated after centrifugation. The CD34+ cell population was isolated using a human CD34+ cell selection kit (StemCell Technologies) according to the manufacturer's instructions. Normal human CD3Full Paper
2017Egidio, C;Gonzales, M;Brockman, J;Chen, S;Durruthy-Durruthy, R;Rogers, C;Ray, M;Abstract LB-078: Improved single-cell mRNA sequencing for transcriptome and paired-chain TCR analysis of primary human CD3+ T cellsCancer ResearchThe low RNA content of single human T cells has limited the success of single-cell transcriptome profiling, an approach that can generate data to reconstruct paired α,β T cell receptor (TCR) chains¹. Single-cell TCR sequencing is a powerful molecularFull Paper
2017Chen, Q;Lawrenz, A;Wang, A;Kelly, E;Harberts, E;Ernst, R;Tong, J;Abstract 4021: Donor-specific primary monocyte-derived dendritic cell maturation model for high-throughput screening of immunostimulatory adjuvantsCancer Research1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++1AllCells, Alameda, CA;++2University of Maryland-Baltimore, Baltimore, MD.++2University of Maryland-Baltimore, Baltimore, MD.++1AllCells, Alameda, CAFull Paper
2017Han, X;Cinay, GE;Zhao, Y;Guo, Y;Zhang, X;Wang, P;Adnectin-Based Design of Chimeric Antigen Receptor for T Cell EngineeringMolecular therapy : the journal of the American Society of Gene Therapy28784559Human PBMCs from healthy donors were obtained from AllCells. PBMCs were cultured in T cell medium (TCM) consisting of X-Vivo 15 (Lonza) supplemented with 5% human AB serum SaveFull Paper
2017Tran, R;Myers, DR;Denning, G;Shields, JE;Lytle, AM;Alrowais, H;Qiu, Y;Sakurai, Y;Li, WC;Brand, O;Le Doux, JM;Spencer, HT;Doering, CB;Lam, WA;Microfluidic Transduction Harnesses Mass Transport Principles to Enhance Gene Transfer EfficiencyMolecular therapy : the journal of the American Society of Gene Therapy28780274vices and wells for at least 72 hr before assessing GFP expression of transduced cells with a BD C6 Accuri flow cytometer. Primary Human T Cell Culture Frozen human pan T cells were purchased from AllCells. Cells were thawed following the AllCells tFull Paper
2017Han, X;Bryson, PD;Zhao, Y;Cinay, GE;Li, S;Guo, Y;Siriwon, N;Wang, P;Masked Chimeric Antigen Receptor for Tumor-Specific ActivationMolecular therapy : the journal of the American Society of Gene Therapy28129121Frozen human peripheral blood mononuclear cells (PBMCs) were obtained from AllCells. PBMCs were thawed in TCM and rested overnight. Before retroviral transduction, PBMCs were SaveFull Paper
2017Liu, X;Rothe, K;Yen, R;Fruhstorfer, C;Maetzig, T;Chen, M;Forrest, DL;Humphries, RK;Jiang, X;A novel AHI-1-BCR-ABL-DNM2 complex regulates leukemic properties of primitive CML cells through enhanced cellular endocytosis and ROS-mediated autophagyLeukemia28366933PB or BM cells were obtained from newly diagnosed patients and healthy adult donors (ALLCELLS). Informed consent was obtained in accordance with the Declaration of Helsinki, and SaveFull Paper
2017Mosoyan, G;Kraus, T;Ye, F;Eng, K;Crispino, JD;Hoffman, R;Iancu-Rubin, C;Imetelstat, a telomerase inhibitor, differentially affects normal and malignant megakaryopoiesisLeukemia28270692Human MKs were generated using a two-step liquid culture system of either bone marrow (BM) or peripheral blood (PB) derived CD34+ cells (AllCells, LLC, Emeryville, CA, USA) as previously described.21,22 CFU-MK assays were performed by using the MegaCFull Paper
2017de Vasconcellos, JF;Tumburu, L;Byrnes, C;Lee, YT;Xu, PC;Li, M;Rabel, A;Clarke, BA;Guydosh, NR;Proia, RL;Miller, JL;IGF2BP1 overexpression causes fetal-like hemoglobin expression patterns in cultured human adult erythroblastsProceedings of the National Academy of Sciences of the United States of America28652347Digestive and Kidney Diseases Institutional Intramural Review Board. CD34(+) cells from adult human healthy volunteers and human cord blood CD34(+) cells obtained from AllCells were studied ex vivo using a serum-free 21-day culture system as previousFull Paper
2017Klinker, MW;Marklein, RA;Lo Surdo, JL;Wei, CH;Bauer, SR;Morphological features of IFN-γ-stimulated mesenchymal stromal cells predict overall immunosuppressive capacityProceedings of the National Academy of Sciences of the United States of America28283659PBMCs used in this study (AllCells; PB006F) were collected from healthy adult human donors. To activate T cells, 10 5 beads coated with anti-CD3 and anti-CD28 (Life Technologies; SaveFull Paper
2017Frescas, D;Roux, CM;Aygun-Sunar, S;Gleiberman, AS;Krasnov, P;Kurnasov, OV;Strom, E;Virtuoso, LP;Wrobel, M;Osterman, AL;Antoch, MP;Mett, V;Chernova, OB;Gudkov, AV;Senescent cells expose and secrete an oxidized form of membrane-bound vimentin as revealed by a natural polyreactive antibodyProceedings of the National Academy of Sciences of the United States of America28193858Human NDFs were purchased from AllCells and maintained in DMEM supplemented with 10% (vol/vol) FBS serum, 100 units/mL of penicillin, 100 µg/mL of streptomycin, and 2 mM l- SaveFull Paper
2017Zhang, H;Gregorio, JD;Iwahori, T;Zhang, X;Choi, O;Tolentino, LL;Prestwood, T;Carmi, Y;Engleman, EG;A distinct subset of plasmacytoid dendritic cells induces activation and differentiation of B and T lymphocytesProceedings of the National Academy of Sciences of the United States of America28167780Stanford Blood Center. Human cord blood and bone marrow samples were purchased from AllCells. Human tonsil cells were obtained from the Stanford Tissue Bank. Human subjects protocols were approved by Stanford’s Institutional Review Board (Palo Alto,Full Paper
2017Nada, MH;Wang, H;Workalemahu, G;Tanaka, Y;Morita, CT;Enhancing adoptive cancer immunotherapy with Vγ2Vδ2 T cells through pulse zoledronate stimulationJournal for immunotherapy of cancer28239463random healthy adult donors were obtained by density centrifugation over Ficoll-Hypaque (GE Healthcare Bio-Sciences Corp., Piscataway, NJ) of either random donor leukopaks (12 donors) (obtained from AllCells, LLC., Alameda, CA or the Blood Donor CentFull Paper
2017Kuramitsu, M;Sekizuka, T;Yamochi, T;Firouzi, S;Sato, T;Umeki, K;Sasaki, D;Hasegawa, H;Kubota, R;Sobata, R;Matsumoto, C;Kaneko, N;Momose, H;Araki, K;Saito, M;Nosaka, K;Utsunomiya, A;Koh, KR;Ogata, M;Uchimaru, K;Iwanaga, M;Sagara, Y;Yamano, Y;Okayama, A;Miura, K;Satake, M;Saito, S;Itabashi, K;Yamaguchi, K;Kuroda, M;Watanabe, T;Okuma, K;Hamaguchi, I;Proviral Features of Human T Cell Leukemia Virus Type 1 in Carriers with Indeterminate Western Blot Analysis ResultsJournal of clinical microbiology28701419AllCells Inc. (Alameda, CA, USA). Cryopreserved PBMCs were resuspended in RPMI 1640 supplemented with 10% FBS at 37C in accordance with the protocol provided by AllCells Inc. SaveFull Paper
2017Gonçalves Silva, I;Yasinska, IM;Sakhnevych, SS;Fiedler, W;Wellbrock, J;Bardelli, M;Varani, L;Hussain, R;Siligardi, G;Ceccone, G;Berger, SM;Ushkaryov, YA;Gibbs, BF;Fasler-Kan, E;Sumbayev, VV;The Tim-3-galectin-9 Secretory Pathway is Involved in the Immune Escape of Human Acute Myeloid Leukemia CellsEBioMedicine28750861Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordance with the Save;ultured in Stem-Pro-34 serum-free media in the presence of 100 ng/ml SCF (KirshenbaFull Paper
2017Alvey, CM;Spinler, KR;Irianto, J;Pfeifer, CR;Hayes, B;Xia, Y;Cho, S;Dingal, PCPD;Hsu, J;Smith, L;Tewari, M;Discher, DE;SIRPA-Inhibited, Marrow-Derived Macrophages Engorge, Accumulate, and Differentiate in Antibody-Targeted Regression of Solid TumorsCurrent biology : CB28669759Subject 1: Female age 30, subject 2: Female age 31, subject 3: Male age 31, subject 4: Male age 22 with additional details provided by AllCells per (Figure 7A). Marrow was pooled and SaveFull Paper
2017Fujishiro, A;Miura, Y;Iwasa, M;Fujii, S;Sugino, N;Andoh, A;Hirai, H;Maekawa, T;Ichinohe, T;Effects of acute exposure to low-dose radiation on the characteristics of human bone marrow mesenchymal stromal/stem cellsInflammation and regeneration29259718e 5E10), and anti-CD105 (clone SN6) antibodies were from eBioscience (San Diego, CA). Isolation, expansion, and γ-irradiation of BM-MSCs BM samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA). BM-MSCs were isolated baFull Paper
2017Yang, C;Wang, X;Ma, B;Zhu, H;Huan, Z;Ma, N;Wu, C;Chang, J;3D-Printed Bioactive Ca3SiO5 Bone Cement Scaffolds with Nano Surface Structure for Bone RegenerationACS applied materials & interfaces28117976Rat bone-marrow stem cells (rBMSCs) (obtained from Allcells, Silicon Valley, CA) were seeded with an initial density of 1× 104 on each scaffold in 48 well tissue culture plates containing 500 μL of Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supFull Paper
2017Gautron, AS;Juillerat, A;Guyot, V;Filhol, JM;Dessez, E;Duclert, A;Duchateau, P;Poirot, L;Fine and Predictable Tuning of TALEN Gene Editing Targeting for Improved T Cell Adoptive ImmunotherapyMolecular therapy. Nucleic acids29246309eBioscience. All the cytometry analyses were performed on a FACSCanto II (BD Biosciences). Cell Culture Frozen peripheral blood mononuclear cells (PBMC) were obtained from healthy volunteer donors (Allcells). T lymphocytes were activated directly frFull Paper
2017Shim, H;Hypoxia-Inducible Factor-1Encyclopedia of CancerIn brief, CD34-depleted human cord blood mononuclear cells (#CB117; AllCells) were infected in vitro with the M81 strain of EBV (2,000 GRU) by incubation at 37C for 1.5 h after which SaveFull Paper
2017Zhang, W;Zhou, L;Shi, J;Deng, H;Fabrication of novel visible-light-driven AgI/g-C(3)N(4) composites with enhanced visible-light photocatalytic activity for diclofenac degradationJournal of colloid and interface science28222304Diclofenac sodium (DCF) was purchased from the Sigma Chemical Reagent Co., US Human umbilical vein endothelial cells (HUVECs) were obtained from Allcells, Shanghai, China. SaveFull Paper
2017Shi, J;Deng, H;Pan, H;Xu, Y;Zhang, M;Epigallocatechin-3-gallate attenuates microcystin-LR induced oxidative stress and inflammation in human umbilical vein endothelial cellsChemosphere27776235Primary HUVECs were obtained from Allcells (Shanghai, China) and cells in passages 3-5 were used in this study. Cells were grown in an RPMI 1640 medium containing 20% FBS ( SaveFull Paper
2017Ali, AJ;Abuelela, AF;Merzaban, JS;An Analysis of Trafficking Receptors Shows that CD44 and P-Selectin Glycoprotein Ligand-1 Collectively Control the Migration of Activated Human T-CellsFrontiers in immunology28515724Frozen normal peripheral blood mononuclear cells isolated from subjects with and without psoriasis were ordered from AllCells (CA, USA). Naive Pan T-cell Isolation Kit and Pan T-cell SaveFull Paper
2017Guo, H;Zhu, Q;Yu, X;Merugu, SB;Mangukiya, HB;Smith, N;Li, Z;Zhang, B;Negi, H;Rong, R;Cheng, K;Wu, Z;Li, D;Tumor-secreted anterior gradient-2 binds to VEGF and FGF2 and enhances their activities by promoting their homodimerizationOncogene28481872HUVECs were purchased from AllCells Company and maintained in HUVEC complete medium (AllCells, Shanghai, China). Cells were passaged for fewer than six passages. THP-1 SaveFull Paper
2017Winter, MB;La Greca, F;Arastu-Kapur, S;Caiazza, F;Cimermancic, P;Buchholz, TJ;Anderl, JL;Ravalin, M;Bohn, MF;Sali, A;O'Donoghue, AJ;Craik, CS;Immunoproteasome functions explained by divergence in cleavage specificity and regulationeLife29182146on the list of commonly misidentified cell lines from the International Cell Line Authentication Committee. Primary human peripheral blood mononuclear cells (PBMCs) (cat. PB005F) were purchased from AllCells (Alameda, CA). The SUP-B15 cell line was pFull Paper
2017Bak, RO;Dever, DP;Reinisch, A;Cruz Hernandez, D;Majeti, R;Porteus, MH;Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR/Cas9 and AAV6eLife28956530Frozen CD34+ HSPCs derived from mobilized peripheral blood or cord blood were purchased from AllCells (Alameda, CA, USA) and thawed according to manufacturer's instructions. SaveFull Paper
2017Kieffer, C;Ladinsky, MS;Ninh, A;Galimidi, RP;Bjorkman, PJ;Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomographyeLife28198699s used at a dilution of 1:500 and 10 nm gold-conjugated goat anti-rabbit IgG (Ted Pella) was used at a dilution of 1:20. Flow cytometry of primary and reconstituted human PBMCs Cultured human PBMCs (AllCells, inc.) were analyzed by flow cytometry (MFull Paper
2017Li, Y;Li, X;Zhao, R;Wang, C;Qiu, F;Sun, B;Ji, H;Qiu, J;Wang, C;Enhanced adhesion and proliferation of human umbilical vein endothelial cells on conductive PANI-PCL fiber scaffold by electrical stimulationMaterials science & engineering. C, Materials for biological applications28024565Human umbilical vein endothelial cells (HUVECs, AllCells Biological Technology Co., Ltd. Shanghai) were cultured in complete Medium. During the cell culture, the culture medium was refreshed every 2- 3 days. HUVECs at passage 2- 3 were used in this wFull Paper
2017Xu, JG;Zhu, SY;Heng, BC;Dissanayaka, WL;Zhang, CF;TGF-β1-induced differentiation of SHED into functional smooth muscle cellsStem cell research & therapy28114966f this study was to differentiate SHED into SMCs by TGF-β1 alone or in combination with BMP4, and to investigate the functionality of the derived SMCs. Methods Cell culture SHED were purchased from ALLCELLS (Alameda, CA, USA), and were evaluated forFull Paper
2017Schlatzer, D;Haqqani, AA;Li, X;Dobrowolski, C;Chance, MR;Tilton, JC;A Targeted Mass Spectrometry Assay for Detection of HIV Gag Protein Following Induction of Latent Viral ReservoirsAnalytical chemistry28467046Normal donor samples were obtained from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated using RosetteSep CD4+ T cell enrichment kit SaveFull Paper
2017Tan, S;Shan, Y;Wang, Y;Lin, Y;Liao, S;Deng, Z;Zhou, L;Cai, W;Zeng, Q;Zhang, L;Zhang, B;Men, X;Li, H;Hu, X;Wu, C;Peng, L;Lu, Z;Exacerbation of oxygen-glucose deprivation-induced blood-brain barrier disruption: potential pathogenic role of interleukin-9 in ischemic strokeClinical science (London, England : 1979)28550144The bEnd.3 cells were purchased from AllCells and grown to subconfluence before the exposure to oxygen-glucose deprivation (OGD: 94.95% N2, 0.05% O2, 5% CO2) or normoxic conditions (75% N2, 20% O2, 5% CO2) for 4 or 24 h. Cells were divided into the fFull Paper
2017Gregg, KA;Harberts, E;Gardner, FM;Pelletier, MR;Cayatte, C;Yu, L;McCarthy, MP;Marshall, JD;Ernst, RK;Rationally Designed TLR4 Ligands for Vaccine Adjuvant DiscoverymBio28487429The dendritic cell (DC) maturation experiments were contracted to and run by AllCells (Alameda, CA) using standard company protocols. Briefly, human PBMCs from 3 separate donors SaveFull Paper
2017Wang, K;Jiang, Z;Webster, KA;Chen, J;Hu, H;Zhou, Y;Zhao, J;Wang, L;Wang, Y;Zhong, Z;Ni, C;Li, Q;Xiang, C;Zhang, L;Wu, R;Zhu, W;Yu, H;Hu, X;Wang, J;Enhanced Cardioprotection by Human Endometrium Mesenchymal Stem Cells Driven by Exosomal MicroRNA-21Stem cells translational medicine28170197ngzhou, China, http://www.sebio.net.cn). Human AdMSCs were purchased from Cyagen Bioscience (Guangzhou, China, http://www.cyagen.com). Human umbilical vein endothelial cells (HUVECs) were provided by AllCells (Shanghai, China, http://www.allcells.comFull Paper
2017Sebastian, NT;Zaikos, TD;Terry, V;Taschuk, F;McNamara, LA;Onafuwa-Nuga, A;Yucha, R;Signer, RAJ;Riddell, J;Bixby, D;Markowitz, N;Morrison, SJ;Collins, KL;CD4 is expressed on a heterogeneous subset of hematopoietic progenitors, which persistently harbor CXCR4 and CCR5-tropic HIV proviral genomes in vivoPLoS pathogens28732051from each donor. All collected samples were coded. Whole umbilical cord blood (CB) from uninfected donors was obtained from the New York Blood Center and whole bone marrow was obtained commercially (AllCells Ltd.). All collected samples were anonymiFull Paper
2017Kraus, RJ;Yu, X;Cordes, BA;Sathiamoorthi, S;Iempridee, T;Nawandar, DM;Ma, S;Romero-Masters, JC;McChesney, KG;Lin, Z;Makielski, KR;Lee, DL;Lambert, PF;Johannsen, EC;Kenney, SC;Mertz, JE;Hypoxia-inducible factor-1α plays roles in Epstein-Barr virus's natural life cycle and tumorigenesis by inducing lytic infection through direct binding to the immediate-early BZLF1 gene promoterPLoS pathogens28617871In brief, CD34-depleted human cord blood mononuclear cells (#CB117; AllCells) were infected in vitro with the M81 strain of EBV (2,000 GRU) by incubation at 37C for 1.5 h after which SaveFull Paper
2017Ganaie, SS;Zou, W;Xu, P;Deng, X;Kleiboeker, S;Qiu, J;Phosphorylated STAT5 directly facilitates parvovirus B19 DNA replication in human erythroid progenitors through interaction with the MCM complexPLoS pathogens28459842ti-viral prophylaxis of transplant recipients. Materials and methods Ethics statement We purchased CD34+ hematopoietic stem cells, which were isolated from bone marrow of a healthy human donor, from AllCells LLC (Alameda, CA) without any identificatFull Paper
2017Nie, Y;Ran, Y;Zhang, HY;Huang, ZF;Pan, ZY;Wang, SY;Wang, YY;GPATCH3 negatively regulates RLR-mediated innate antiviral responses by disrupting the assembly of VISA signalosomePLoS pathogens28414768University, Cambridge, MA). HEK293T (Transformed Human Embryonic Kidney 293 cell line, ATCC) cells, A549 (Human Lung Adenocarcinoma cell line, ATCC) cells, PBMCs (Peripheral Blood Mononuclear Cells, Allcells), Human recombinant TNFα (R&D Systems), poFull Paper
2017Xu, P;Zhou, Z;Xiong, M;Zou, W;Deng, X;Ganaie, SS;Kleiboeker, S;Peng, J;Liu, K;Wang, S;Ye, SQ;Qiu, J;Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathwayPLoS pathogens28264028We purchased the cells at AllCells LLC. (www.allcells.com, Alameda, CA), which were all anonymized, and, therefore, an IRB review was waived. SaveFull Paper
2017Morin, J;Dubey, N;Decroix, F;Luong-Van, E;Castro Neto, A;Rosa, V;Graphene transfer to 3-dimensional surfaces: a vacuum-assisted dry transfer method2D MaterialsDental pulp stem cells (DPSC) (AllCells, USA) of passage 4 were cultured in Dulbecco’s modified Eagle’s medium (Invitrogen, USA), supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin/streptomycin (Invitrogen). Disinfection of the iFull Paper
2017Ma, SD;Tsai, MH;Romero-Masters, JC;Ranheim, EA;Huebner, SM;Bristol, JA;Delecluse, HJ;Kenney, SC;Latent Membrane Protein 1 (LMP1) and LMP2A Collaborate To Promote Epstein-Barr Virus-Induced B Cell Lymphomas in a Cord Blood-Humanized Mouse Model but Are Not EssentialJournal of virology28077657Commercially purchased CD34depleted human cord blood mononuclear cells (CB117; AllCells, LLC) were mock infected or infected with WT or mutant viruses in vitro for 1.5 h, and 12 SaveFull Paper
2017Adler, AS;Mizrahi, RA;Spindler, MJ;Adams, MS;Asensio, MA;Edgar, RC;Leong, J;Leong, R;Roalfe, L;White, R;Goldblatt, D;Johnson, DS;Rare, high-affinity anti-pathogen antibodies from human repertoires, discovered using microfluidics and molecular genomicsmAbs28846502For influenza A vaccinated libraries, we contracted AllCells to vaccinate three donors (ages For the pneumococcus vaccinated libraries, we contracted AllCells to vaccinate five donors ( SaveFull Paper
2017Urbinati, F;Wherley, J;Geiger, S;Fernandez, BC;Kaufman, ML;Cooper, A;Romero, Z;Marchioni, F;Reeves, L;Read, E;Nowicki, B;Grassman, E;Viswanathan, S;Wang, X;Hollis, RP;Kohn, DB;Preclinical studies for a phase 1 clinical trial of autologous hematopoietic stem cell gene therapy for sickle cell diseaseCytotherapy28733131Control healthy donor BM samples were purchased from commercial sources (AllCells or Hemacare) obtaining aspirates from volunteer donors. The ages of the five healthy donors SaveFull Paper
2017Pachler, K;Lener, T;Streif, D;Dunai, ZA;Desgeorges, A;Feichtner, M;Öller, M;Schallmoser, K;Rohde, E;Gimona, M;A Good Manufacturing Practice-grade standard protocol for exclusively human mesenchymal stromal cell-derived extracellular vesiclesCytotherapy28188071The second BM aspirate was purchased from AllCells. Both donors had signed an informed consent. BM-MSCs were isolated from BM as described [30] and tested negative for SaveFull Paper
2017Khairani, M;Nur, N;Basma, M;Widya, L;Solachuddin Jauhari, I;Deny, D;Azlina, A;Thirumulu, K;Effects of flaxseed extract on the viability of stem cells from human extracted deciduous teeth (SHED)Frontiers in Bioengineering and BiotechnologySHED (AllCells, USA) was previously cultured and maintained in alpha-MEM (GIBCO, USA) containing 10% FBS (GIBCO, USA) and 0.5% penicillin/streptomycin (GIBCO, USA) until SaveFull Paper
2017Lifshitz, V;Priceman, SJ;Li, W;Cherryholmes, G;Lee, H;Makovski-Silverstein, A;Borriello, L;DeClerck, YA;Yu, H;Sphingosine-1-Phosphate Receptor-1 Promotes Environment-Mediated and Acquired ChemoresistanceMolecular cancer therapeutics28716816Human BM-MSC were either purchased from ALLCells LLC or obtained from fresh ALLCells, which were maintained in basal medium with growth supplements obtained from ALLCells. SaveFull Paper
2017Zhao, H;Gulesserian, S;Ganesan, SK;Ou, J;Morrison, K;Zeng, Z;Robles, V;Snyder, J;Do, L;Aviña, H;Karki, S;Stover, DR;Doñate, F;Inhibition of Megakaryocyte Differentiation by Antibody-Drug Conjugates (ADCs) is Mediated by Macropinocytosis: Implications for ADC-induced ThrombocytopeniaMolecular cancer therapeutics28655784ABM026F) isolated from bone marrow were purchased from ALLCELLS. All HSC growth media (StemSpan SFEM II, catalog no. 09600), supplements for expansion (CC100, catalog no. SaveFull Paper
2017Ao, JY;Zhu, XD;Chai, ZT;Cai, H;Zhang, YY;Zhang, KZ;Kong, LQ;Zhang, N;Ye, BG;Ma, DN;Sun, HC;Colony-Stimulating Factor 1 Receptor Blockade Inhibits Tumor Growth by Altering the Polarization of Tumor-Associated Macrophages in Hepatocellular CarcinomaMolecular cancer therapeutics28572167A human umbilical vein endothelial cell (HUVEC) line was purchased from Allcells and was grown in Allcells completed medium supplemented with 10% FBS. All cell lines obtained SaveFull Paper
2017Zhao, H;Gulesserian, S;Malinao, MC;Ganesan, SK;Song, J;Chang, MS;Williams, MM;Zeng, Z;Mattie, M;Mendelsohn, BA;Stover, DR;Doñate, F;A Potential Mechanism for ADC-Induced Neutropenia: Role of Neutrophils in Their Own DemiseMolecular cancer therapeutics28522588Human hematopoietic stem cells (CD133+, catalog no. ABM026F) isolated from bone marrow and human mature neutrophils (catalog no. PB016) were purchased from ALLCELLS. HSC growth media (StemSpan SFEM II, catalog no. 09600), supplements for expansion (CFull Paper
2017Tao, B;Wang, R;Sun, C;Zhu, Y;3-Mercaptopyruvate Sulfurtransferase, Not Cystathionine β-Synthase Nor Cystathionine γ-Lyase, Mediates Hypoxia-Induced Migration of Vascular Endothelial CellsFrontiers in pharmacology28979207et al., 2007). Materials and methods Cell culture Primary human umbilical vein endothelial cells (HUVECs) and culture medium (supplemented with or without 10% fetal bovine serum) were purchased from Allcells (Shanghai, China). Cells were cultured inFull Paper
2017Hall, BM;Balan, V;Gleiberman, AS;Strom, E;Krasnov, P;Virtuoso, LP;Rydkina, E;Vujcic, S;Balan, K;Gitlin, II;Leonova, KI;Consiglio, CR;Gollnick, SO;Chernova, OB;Gudkov, AV;p16(Ink4a) and senescence-associated β-galactosidase can be induced in macrophages as part of a reversible response to physiological stimuliAging28768895enmark) where live and dead counts of nucleated cells were quantitated on a NucleoCounter NC-200 (ChemoMetec) via staining with acridine orange and DAPI. Primary human neonatal dermal fibroblasts (AllCells, LLC; Alameda, CA), pooled equally from thFull Paper
2017Bam, RA;Hansen, D;Irrinki, A;Mulato, A;Jones, GS;Hesselgesser, J;Frey, CR;Cihlar, T;Yant, SR;TLR7 Agonist GS-9620 Is a Potent Inhibitor of Acute HIV-1 Infection in Human Peripheral Blood Mononuclear CellsAntimicrobial agents and chemotherapy27799218e anti-human CD8 antibodies, used for flow cytometry experiments, were purchased from BD Biosciences (San Jose, CA, USA) and stored at 4°C in the dark. Primary cells. Leucopacks were purchased from AllCells (Alameda, CA, USA) and were obtained fromFull Paper
2017Kota, DJ;Prabhakara, KS;Toledano-Furman, N;Bhattarai, D;Chen, Q;DiCarlo, B;Smith, P;Triolo, F;Wenzel, PL;Cox, CS;Olson, SD;Prostaglandin E2 Indicates Therapeutic Efficacy of Mesenchymal Stem Cells in Experimental Traumatic Brain InjuryStem cells (Dayton, Ohio)28233425Donors 3305, 3388 P0 MSC were isolated from commercially available fresh human bone marrow (hBM) aspirates (AllCells, Emeryville, CA, https://www.allcells.com/); MSC from donor TBM-1 were obtained from hBM aspirates under the approved IBC protocol #Full Paper
2017Diaz, MF;Vaidya, AB;Evans, SM;Lee, HJ;Aertker, BM;Alexander, AJ;Price, KM;Ozuna, JA;Liao, GP;Aroom, KR;Xue, H;Gu, L;Omichi, R;Bedi, S;Olson, SD;Cox, CS;Wenzel, PL;Biomechanical Forces Promote Immune Regulatory Function of Bone Marrow Mesenchymal Stromal CellsStem cells (Dayton, Ohio)28181347Bone marrow MSCs were derived from whole bone marrow from independent human donors (commercially available by AllCells) (Supporting Information Figure S1A). Briefly, mononuclear cells were enriched in the buffy layer of whole bone marrow by phase sepFull Paper
2017Garbuzova-Davis, S;Haller, E;Lin, R;Borlongan, CV;Intravenously Transplanted Human Bone Marrow Endothelial Progenitor Cells Engraft Within Brain Capillaries, Preserve Mitochondrial Morphology, and Display Pinocytotic Activity Toward Blood-Brain Barrier Repair in Ischemic Stroke RatsStem cells (Dayton, Ohio)28142208The isolation of human bone marrow endothelial progenitor cells (hBMEPCs) was performed accordingly to the protocol reported previously [52-54] with modifications. Briefly, for each independent experiment, human bone marrows (AllCells, Almeda, CA, htFull Paper
2017Zhang, M;Pan, H;Xu, Y;Wang, X;Qiu, Z;Jiang, L;Allicin Decreases Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Umbilical Vein Endothelial Cells through Suppression of Mitochondrial Dysfunction and Activation of Nrf2Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology28456799Commercial HUVECs were purchased from Allcells (Shanghai Biological Technology Co., Ltd., China). The cells were seeded in RPMI 1640 medium with 10 % fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) and 60?µg/mL endothelial cell growth supplemeFull Paper
2017Deng, H;Song, Z;Xu, H;Deng, X;Zhang, Q;Chen, H;Wang, Y;Qin, Y;Li, Y;MicroRNA-1185 Promotes Arterial Stiffness though Modulating VCAM-1 and E-Selectin ExpressionCellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology28441665The pHUVECs were obtained from AllCells (Shanghai, China) and cultured in complete medium (AllCells, Shanghai, China). The HUVSMCs were purchased from ScienCell (Carlsbad, SaveFull Paper
2017Deng, H;Chu, X;Song, Z;Deng, X;Xu, H;Ye, Y;Li, S;Zhang, Q;Sun, C;Li, Y;MicroRNA-1185 Induces Endothelial Cell Apoptosis by Targeting UVRAG and KRIT1Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology28441650pHUVEC were purchased from Allcells (Shanghai, China) and maintained in complete medium (Allcells). The human endothelial cells line EA.hy926 cells (ATCC, Manassas, VA, USA) SaveFull Paper
2017Shay, AE;Diwakar, BT;Guan, BJ;Narayan, V;Urban, JF;Prabhu, KS;IL-4 up-regulates cyclooxygenase-1 expression in macrophagesThe Journal of biological chemistry28684424Frozen human PBMC were purchased from AllCells (Alameda, CA) from donors. Upon arrival, cells were thawed in a 37 °C water and enumerated with trypan blue staining using a hemocytometer to determine cell viability. Cells were transferred to a 50-ml tFull Paper
2017Li, X;Anderson, M;Collin, D;Muegge, I;Wan, J;Brennan, D;Kugler, S;Terenzio, D;Kennedy, C;Lin, S;Labadia, ME;Cook, B;Hughes, R;Farrow, NA;Structural studies unravel the active conformation of apo RORγt nuclear receptor and a common inverse agonism of two diverse classes of RORγt inhibitorsThe Journal of biological chemistry28546429Frozen CD4+ T cells (AllCells) were thawed and resuspended in X-VIVO media (Lonza) at a cell density of 1 × 106 cells/ml. Skewing cytokines were added to media at the final concentrations of 30 ng/ml IL-23, 10 ng/ml IL-1β, 10 ng/ml IL-6, 2 ng/ml IL-2Full Paper
2017Li, J;Bhattacharya, S;Zhou, J;Phadnis-Moghe, AS;Crawford, RB;Kaminski, NE;Aryl Hydrocarbon Receptor Activation Suppresses EBF1 and PAX5 and Impairs Human B LymphopoiesisJournal of immunology (Baltimore, Md. : 1950)28978690Fresh human CD34 + HSPCs isolated from cord blood from mixed donors were purchased from AllCells (Emeryville, CA). The in vitro feeder-free HSPC culture was modified based on a SaveFull Paper
2017Eskandarpour, M;Alexander, R;Adamson, P;Calder, VL;Pharmacological Inhibition of Bromodomain Proteins Suppresses Retinal Inflammatory Disease and Downregulates Retinal Th17 CellsJournal of immunology (Baltimore, Md. : 1950)28039300Enriched human PBMC-derived CD4+ T cells were obtained (AllCells; Caltag Medsystems, Buckingham, U.K.) and cultured in X-VIVO 20 and polarized to a Th17 phenotype by addition of recombinant human (rh)IL-6 (30 ng/ml), rhIL-23 (20 ng/ml), rhIL-1b (10 nFull Paper
2017Frescas, D;Hall, BM;Strom, E;Virtuoso, LP;Gupta, M;Gleiberman, AS;Rydkina, E;Balan, V;Vujcic, S;Chernova, OB;Gudkov, AV;Murine mesenchymal cells that express elevated levels of the CDK inhibitor p16(Ink4a) in vivo are not necessarily senescentCell cycle (Georgetown, Tex.)28650766Human NDFs were purchased from AllCells and maintained in DMEM supplemented with 10% FBS serum, 100 units/mL of penicillin, 100 µg/mL of streptomycin, and 2 mM L-Glutamine SaveFull Paper
2017Li, S;Ning, H;Ye, Y;Wei, W;Guo, R;Song, Q;Liu, L;Liu, Y;Na, L;Niu, Y;Chu, X;Feng, R;Moustaid-Moussa, N;Li, Y;Sun, C;Increasing extracellular Ca2+ sensitizes TNF-alpha-induced vascular cell adhesion molecule-1 (VCAM-1) via a TRPC1/ERK1/2/NFκB-dependent pathway in human vascular endothelial cellsBiochimica et biophysica acta. Molecular cell research28583863Primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Cells were used between passages 3 and 6. Human umbilical vein cell line (EA.hy926) and human leukemic monocyte lymphoma cell line (U937) were obtFull Paper
2017Wang, H;Gonzalez-Garcia, I;Traba, J;Jain, S;Conteh, S;Shin, DM;Qi, C;Gao, Y;Sun, J;Kang, S;Abbasi, S;Naghashfar, Z;Yoon, J;DuBois, W;Kovalchuk, AL;Sack, MN;Duffy, P;Morse, HC;ATP-degrading ENPP1 is required for survival (or persistence) of long-lived plasma cellsScientific reports29259245The efficiency of reconstitution was verified by FACS analyses of blood for frequencies of B and T cells shortly before mice were immunized 8 wks later. Human bone marrow cells were purchased from Allcells (Alameda, CA) and processed for antibody stFull Paper
2017Katano, I;Nishime, C;Ito, R;Kamisako, T;Mizusawa, T;Ka, Y;Ogura, T;Suemizu, H;Kawakami, Y;Ito, M;Takahashi, T;Long-term maintenance of peripheral blood derived human NK cells in a novel human IL-15- transgenic NOG mouseScientific reports29222435iminated twice using a MACS LD column (Miltenyi Biotec). The purity of the isolated human NK cells was usually greater than 95%. We also used human Caucasian PB-derived CD56+ NK cells, purchased from AllCells (Berkeley, CA, USA), in some experiments.Full Paper
2017Zhang, M;Qiu, L;Zhang, Y;Xu, D;Zheng, JC;Jiang, L;CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cellsScientific reports28811579Human umbilical cord vein endothelial cells (HUVECs) (AllCells, Shanghai, China) were cultured for up to 5-7 passages in complete endothelial cell growth medium (H004, AllCells, SaveFull Paper
2017Ma, R;Xie, R;Yu, C;Si, Y;Wu, X;Zhao, L;Yao, Z;Fang, S;Chen, H;Novakovic, V;Gao, C;Kou, J;Bi, Y;Thatte, HS;Yu, B;Yang, S;Zhou, J;Shi, J;Phosphatidylserine-mediated platelet clearance by endothelium decreases platelet aggregates and procoagulant activity in sepsisScientific reports28694452a50,51. Study was performed with the approval of the Ethics Committee of Harbin Medical University in accordance with the Declaration of Helsinki. Reagents Endothelial cell medium was purchased from AllCells (Emeryville, CA, USA). Bovine serum albumFull Paper
2017Garbuzova-Davis, S;Kurien, C;Thomson, A;Falco, D;Ahmad, S;Staffetti, J;Steiner, G;Abraham, S;James, G;Mahendrasah, A;Sanberg, PR;Borlongan, CV;Endothelial and Astrocytic Support by Human Bone Marrow Stem Cell Grafts into Symptomatic ALS Mice towards Blood-Spinal Cord Barrier RepairScientific reports28408761t 14 weeks of age and weekly thereafter, mice again underwent behavioral testing until 17 weeks of age. Cell preparation and transplant procedure Cryopreserved human bone marrow CD34+ cells (hBM34+, AllCells, Alameda, CA, USA) were thawed rapidly atFull Paper
2017Li, XH;Xue, WL;Wang, MJ;Zhou, Y;Zhang, CC;Sun, C;Zhu, L;Liang, K;Chen, Y;Tao, BB;Tan, B;Yu, B;Zhu, YC;H2S regulates endothelial nitric oxide synthase protein stability by promoting microRNA-455-3p expressionScientific reports28322298Primary human umbilical vein endothelial cells were purchased from Allcells (Shanghai, China) and cultured in endothelial complete medium (ECM; Allcells, China) as described SaveFull Paper
2017Fang, HY;Zeng, HW;Lin, LM;Chen, X;Shen, XN;Fu, P;Lv, C;Liu, Q;Liu, RH;Zhang, WD;Zhao, J;A network-based method for mechanistic investigation of Shexiang Baoxin Pill's treatment of cardiovascular diseasesScientific reports28272527HUVECs were seeded into 60 mm cell culture plates with complete endothelial growth medium (AllCells). After reaching 80% confluence, cells were exposed to SBP extract and SBPpc SaveFull Paper
2017Manser, M;Sater, MR;Schmid, CD;Noreen, F;Murbach, M;Kuster, N;Schuermann, D;Schär, P;ELF-MF exposure affects the robustness of epigenetic programming during granulopoiesisScientific reports28266526CD34-positive cells isolated from human cord blood of mixed donors were obtained from AllCells (Alabama, United States) and propagated in Stemline II medium expansion medium ( SaveFull Paper
2017Wei, G;Jie, Y;Haibo, L;Chaoneng, W;Dong, H;Jianbing, Z;Junjie, G;Leilei, M;Hongtao, S;Yunzeng, Z;Junbo, G;Dendritic cells derived exosomes migration to spleen and induction of inflammation are regulated by CCR7Scientific reports28223684ons (6 μm) were prepared using a Lab-Tek tissue processor (Leica). The samples were stained with CD11c/CD4 (Abcam) and DAPI and observed with confocal microscopy. CD4+ T cells were purchased from Allcells (Shanghai, China). To determine whether T ceFull Paper
2017Rodriguez, C;Kessler, F;Dubey, N;Rosa, V;Fechine, G;CVD graphene transfer procedure to the surface of stainless steel for stem cell proliferationSurface and Coatings TechnologyHuman dental pulp stem cells (DPSC) from a single donor (DPF003, Allcells, USA) were cultured in Dulbecco's modified Eagle's medium (Invitrogen, USA), supplemented with 10% SaveFull Paper
2017Lee, HJ;Diaz, MF;Ewere, A;Olson, SD;Cox, CS;Wenzel, PL;Focal adhesion kinase signaling regulates anti-inflammatory function of bone marrow mesenchymal stromal cells induced by biomechanical forceCellular signalling28647573Bone marrow MSCs were derived from whole bone marrow from independent human donor (AllCells). Cells were isolated and maintained as described previously [26]. Briefly, enriched SaveFull Paper
2017Killilea, DW;Rohner, F;Ghosh, S;Otoo, GE;Smith, L;Siekmann, JH;King, JC;Identification of a Hemolysis Threshold That Increases Plasma and Serum Zinc ConcentrationThe Journal of nutrition28490675Blood samples from 14 healthy participants (Supplemental Table 1) were purchased from a commercial laboratory (AllCells LLC). The laboratory prescreened donors for lack of SaveFull Paper
2017Bellayr, IH;Kumar, A;Puri, RK;MicroRNA expression in bone marrow-derived human multipotent Stromal cellsBMC genomics28800721A total of 8 human bone marrow-derived MSCs from 8 donors were purchased from ALLCELLS and Lonza at passage 1 (Additional file 1: Table S1). By flow cytometry, all MSCs SaveFull Paper
2017Huang, J;Khan, A;Au, BC;Barber, DL;López-Vásquez, L;Prokopishyn, NL;Boutin, M;Rothe, M;Rip, JW;Abaoui, M;Nagree, MS;Dworski, S;Schambach, A;Keating, A;West, ML;Klassen, J;Turner, PV;Sirrs, S;Rupar, CA;Auray-Blais, C;Foley, R;Medin, JA;Lentivector Iterations and Pre-Clinical Scale-Up/Toxicity Testing: Targeting Mobilized CD34+ Cells for Correction of Fabry DiseaseMolecular therapy. Methods & clinical development28603745lutamine (PSQ) (2 mM glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin; Gibco by Life Technologies). Mobilized peripheral blood normal CD34+ cells from healthy donors were purchased from AllCells. Human primary CD34+ cells were cultured inFull Paper
2017Lawson, T;Kehoe, D;Schnitzler, A;Rapiejko, P;Der, K;Philbrick, K;Punreddy, S;Rigby, S;Smith, R;Feng, Q;Murrell, J;Rook, M;Process development for expansion of human mesenchymal stromal cells in a 50L single-use stirred tank bioreactorBiochemical Engineering JournalPBMCs (AllCells, Alameda, CA) that had been thawed and incubated overnight in 3 mL RPMI medium in ultra-low attachment 6-well plates (Corning) were stained with CFDA-SE (Thermo Fisher Scientific), according to the manufacturer’s recommendations. ForFull Paper
2017Heng, BC;Gong, T;Wang, S;Lim, LW;Wu, W;Zhang, C;Decellularized Matrix Derived from Neural Differentiation of Embryonic Stem Cells Enhances the Neurogenic Potential of Dental Follicle Stem CellsJournal of endodontics28231979The hESCs (WA01 line) were obtained from Wicell Institute (Madison, WI). Human DFSCs (catalog no. FT002 F, lot no. 020609) were purchased from AllCells LLC (Alameda, CA). Unless mentioned otherwise, all cell culture media and supplements were procureFull Paper
2017Elwood, F;Witter, DJ;Piesvaux, J;Kraybill, B;Bays, N;Alpert, C;Goldenblatt, P;Qu, Y;Ivanovska, I;Lee, HH;Chiu, CS;Tang, H;Scott, ME;Deshmukh, SV;Zielstorff, M;Byford, A;Chakravarthy, K;Dorosh, L;Rivkin, A;Klappenbach, J;Pan, BS;Kariv, I;Dinsmore, C;Slipetz, D;Dandliker, PJ;Evaluation of JAK3 Biology in Autoimmune Disease Using a Highly Selective, Irreversible JAK3 InhibitorThe Journal of pharmacology and experimental therapeutics28193636Frozen, purified human CD34+ from mobilized blood (AllCells) were seeded at 100,000 cell/ml in Stempro34 complete medium (Thermo Fisher) supplemented with GMCSF at 20 ng/ml (Thermo Fisher), human stem cell factor at 40 ng/ml (Cell Signaling), and IL-Full Paper
2017Erickson, RI;Schutt, LK;Tarrant, JM;McDowell, M;Liu, L;Johnson, AR;Lewin-Koh, SC;Hedehus, M;Ross, J;Carano, RA;Staflin, K;Zhong, F;Crawford, JJ;Zhong, S;Reif, K;Katewa, A;Wong, H;Young, WB;Dambach, DM;Misner, DL;Bruton's Tyrosine Kinase Small Molecule Inhibitors Induce a Distinct Pancreatic Toxicity in RatsThe Journal of pharmacology and experimental therapeutics27821712samples, sex unknown; Prodo Laboratories, Irvine, CA), universal RNA (rat/human; Clontech, Mountain View, CA), and total RNA from pooled isolated rat islet preparations (AllCells, SaveFull Paper
2017Ye, J;Si, J;Cui, Z;Wang, Q;Peng, K;Chen, W;Peng, X;Chen, S;Surface Modification of Electrospun TPU Nanofiber Scaffold with CNF Particles by Ultrasound-Assisted Technique for Tissue EngineeringMacromolecular Materials and EngineeringThe cell viability of the TPU and CNF-adsorbed TPU nanofiber was determined using a MTT assay (3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide). Human umbilical vein endothelial cells (HUVECs, AllCells Biological Technology Co., Ltd.,Full Paper
2017McBride, JD;Rodriguez-Menocal, L;Guzman, W;Candanedo, A;Garcia-Contreras, M;Badiavas, EV;Bone Marrow Mesenchymal Stem Cell-Derived CD63+ Exosomes Transport Wnt3a Exteriorly and Enhance Dermal Fibroblast Proliferation, Migration, and Angiogenesis In VitroStem cells and development28679315BM-MSC "line 2" and "line 3" were obtained from healthy male and female donors, respectively, according to quality control and manufacturing established by AllCells , and isolated SaveFull Paper
2017Setrerrahmane, S;Yu, J;Hao, J;Zheng, H;Xu, H;Novel production method of innovative antiangiogenic and antitumor small peptides in Escherichia coliDrug design, development and therapy29184391om GE Healthcare Life Sciences (Logan, UT, USA) and used for gene expression and protein purification, respectively. Human umbilical vein endothelial cells (HUVECs; primary cells) were purchased from AllCells (Shanghai, People’s Republic of China); aFull Paper
2017d Johnston, IC;Wagemaker, G;development of a highly efficient lentiviral gene transfer protocol for hematopoietic stem cellsBookBone marrow samples were purchased from AllCells (Alameda, USA). These studies were performed according to established ethical guidelines, and all blood donors gave informed SaveFull Paper
2017Ito, R;Nagai, D;Igo, N;Okuda, Y;Sekine, K;Ichimura, E;Katano, I;Mizushima, T;Goto, M;Ohnishi, Y;Ito, M;Okamoto, K;A novel in vivo model for predicting myelotoxicity of chemotherapeutic agents using IL-3/GM-CSF transgenic humanized miceToxicology letters28947270Commercially available human cord blood (CB)-derived CD34+ cells obtained from a single donor were purchased from Lonza (Basel, Switzerland) or Allcells (Alameda, CA, USA). The cells were prepared using a standard protocol for xenotransplantationFull Paper
2017FUKAHORI, H;Investigation Regarding the Properties of Protein Kinase Cθ Selective Inhibitor as a Novel T cell ImmunosuppressantThesisT cell proliferation assay Human peripheral T cells were purchased from AllCells LLC (Alameda, CA, USA). Cells (1 x 105) suspended in RPMI 1640 containing 10% fetal bovine serum SaveFull Paper
2017Jin, J;Wang, Y;Xu, Y;Zhou, X;Liu, Y;Li, X;Wang, J;MicroRNA-144 regulates cancer cell proliferation and cell-cycle transition in acute lymphoblastic leukemia through the interaction of FMN2The journal of gene medicine27556228ALL cell lines, Molt-3, Molt-4, Tall-104, Jurkat, CCRF-CEM, CEM/C1 and CEM/C2, were purchased from American Type Culture Collection (American Type Culture Collection, Manassas, VA, USA). A normal peripheral blood pan-T cell line (BPBPT) was purchasedFull Paper
2017Heck, BE;Park, JJ;Makani, V;Kim, EC;Kim, DH;PPAR-δ Agonist With Mesenchymal Stem Cells Induces Type II Collagen-Producing Chondrocytes in Human Arthritic Synovial FluidCell transplantation28901183To eliminate donor-to-donor variability, we used synovial fluid from 3 different OA patients and used BM-MSCs from 3 donors (AllCells) to measure the GAG content. The use of human SaveFull Paper
2017Bender, AT;Gardberg, A;Pereira, A;Johnson, T;Wu, Y;Grenningloh, R;Head, J;Morandi, F;Haselmayer, P;Liu-Bujalski, L;Ability of Bruton's Tyrosine Kinase Inhibitors to Sequester Y551 and Prevent Phosphorylation Determines Potency for Inhibition of Fc Receptor but not B-Cell Receptor SignalingMolecular pharmacology28062735E for 15 minutes and then measures CD63 upregulation by flow cytometry. Human cord blood isolated CD341 stem cells (AllCells, Alameda, CA) were differentiated to mast cells by culture for 8 weeks. Cells were maintained in StemPro-34 media (Gibco, WalFull Paper
2017Fujisawa, K;Takami, T;Fukui, Y;Quintanilha, LF;Matsumoto, T;Yamamoto, N;Sakaida, I;Evaluating effects of L-carnitine on human bone-marrow-derived mesenchymal stem cellsCell and tissue research28197778The studied BmMSCs were bone marrow cells purchased from Allcells and were continuously cultured and used at passage 4 and passage 16. Flow cytometry results confirmed that SaveFull Paper
2017Bai, L;Li, Q;Duo, X;Hao, X;Zhang, W;Shi, C;Guo, J;Ren, X;Feng, Y;Electrospun PCL-PIBMD/SF blend scaffolds with plasmid complexes for endothelial cell proliferationRSC Advances2.6.2. Cell culture.Human umbilical vein endothelial cells (HUVECs) were bought from Allcells Biomart (Shanghai, China) and cultured in Dulbecco's modified Eagle medium (DMEM, Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Gibco), 100 μgFull Paper
2017Dong, B;Zhang, X;Fan, Y;Cao, S;Zhang, X;GPR4 knockout improves renal ischemia-reperfusion injury and inhibits apoptosis via suppressing the expression of CHOPThe Biochemical journal29089376Cell culture and treatment Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCELLS (Emeryville, CA, U.S.A.). Cells were cultured in Dulbecco's modified Eagle medium (Gibco, U.S.A.) with 10% fetal bovine serum (Gibco, U.S.A.) at 3Full Paper
2017Li, S;Yuan, H;Pan, J;Fan, W;Zhu, L;Yan, Z;Guo, C;The treatment of femoral neck fracture using VEGF-loaded nanographene coated internal fixation screwsPloS one29117211The human umbilical vein endothelial cells (HUVECs) (Allcells, Alameda, CA, USA) and osteoblasts (Type culture collection of the Chinese Academy of Sciences, Shanghai, China) SaveFull Paper
2017Sewing, S;Roth, AB;Winter, M;Dieckmann, A;Bertinetti-Lapatki, C;Tessier, Y;McGinnis, C;Huber, S;Koller, E;Ploix, C;Reed, JC;Singer, T;Rothfuss, A;Assessing single-stranded oligonucleotide drug-induced effects in vitro reveals key risk factors for thrombocytopeniaPloS one291079690 sec after the end of the association phase in the binding sensorgram were evaluated to rank ONs. Human bone marrow toxicity assay Human mononucleated cells (hMNCs) were received cryopreserved from AllCells, LLC or STEMCELL Technologies Inc, and weFull Paper
2017Ross, SL;Sherman, M;McElroy, PL;Lofgren, JA;Moody, G;Baeuerle, PA;Coxon, A;Arvedson, T;Bispecific T cell engager (BiTE®) antibody constructs can mediate bystander tumor cell killingPloS one28837681Pan-T cells were purchased from AllCells. T cells that were not stimulated prior to use in assays are referred to as resting T cells. Activated T cells were generated by two methods: (1) SaveFull Paper
2017Liu, L;Koo, Y;Collins, B;Xu, Z;Sankar, J;Yun, Y;Biodegradability and platelets adhesion assessment of magnesium-based alloys using a microfluidic systemPloS one28797069The product was purchased from Allcells with the concentration of 5 x 10 8 /ml. PRP was used immediately upon arrival. Micro-Renathane tubing (0.066-in. SaveFull Paper
2017Graci, JD;Michaels, D;Chen, G;Schiralli Lester, GM;Nodder, S;Weetall, M;Karp, GM;Gu, Z;Colacino, JM;Henderson, AJ;Identification of benzazole compounds that induce HIV-1 transcriptionPloS one28658263Brunswick, NJ (UMDNJ, now part of Rutgers University)[48]. ACH-2 and U1 cells were obtained through the NIH AIDS Reagent Program, Division of AIDS, NIAID, NIH. Cryopreserved PBMCs were purchased from AllCells, LLC (Alameda, CA). RPMI-1640, DMEM, hygrFull Paper
2017Salzberg, AC;Harris-Becker, A;Popova, EY;Keasey, N;Loughran, TP;Claxton, DF;Grigoryev, SA;Genome-wide mapping of histone H3K9me2 in acute myeloid leukemia reveals large chromosomal domains associated with massive gene silencing and sites of genome instabilityPloS one28301528Cryopreserved bone marrow CD34+ cells from normal donors were obtained from Allcells (Alameda, CA). Bone marrow CD34+ cells represent a combination of stem and progenitor SaveFull Paper
2017Li, Y;He, Q;Hu, X;Liu, Y;Cheng, X;Li, X;Deng, F;Improved performance of collagen scaffolds crosslinked by Traut's reagent and Sulfo-SMCCJournal of biomaterials science. Polymer edition28277011The HUVECs used in this study were purchased from Allcells (Shanghai). HUVECs were cultured with endothelial complete medium (Allcells, Shanghai) in an incubator with 5% CO 2 SaveFull Paper
2017Tharinger, H;Rebbapragada, I;Samuel, D;Novikov, N;Nguyen, MH;Jordan, R;Frey, CR;Pflanz, S;Antibody-dependent and antibody-independent uptake of HBsAg across human leucocyte subsets is similar between individuals with chronic hepatitis B virus infection and healthy donorsJournal of viral hepatitis28012213Peripheral blood from healthy donors (HD) was purchased from AllCells (Alameda, CA, USA). Informed written consent was obtained from all enrolled study subjects prior to blood draw. Additional details related to subject enrolment can be found in suppFull Paper
2017Bonar, MM;Tilton, JC;High sensitivity detection and sorting of infectious human immunodeficiency virus (HIV-1) particles by flow virometryVirology28235684This study was conducted according to the principles specified in the Declaration of Helsinki and under local ethical guidelines (Case Western Reserve University Institutional Review Board (IRB)). Normal donor samples were de-identified and obtainedFull Paper
2017Chen, S;Wu, X;Hu, J;Dai, G;Rong, A;Guo, G;PM2.5 exposure decreases viability, migration and angiogenesis in human umbilical vein endothelial cells and human microvascular endothelial cellsMolecular medicine reports28677750HUVECs and HMEC-1 human microvascular endothelial cells were obtained from AllCells, LLC (H-001F; Shanghai, China). Cells were cultured in RPMI 1640 medium with 20% FBS, SaveFull Paper
2017DiCarlo, AL;Tamarat, R;Rios, CI;Benderitter, M;Czarniecki, CW;Allio, TC;Macchiarini, F;Maidment, BW;Jourdain, JR;Cellular Therapies for Treatment of Radiation Injury: Report from a NIH/NIAID and IRSN WorkshopRadiation research28605260having sources of ready-to-inject cells facilitates research. Although cells can be obtained from various commercial sources (e.g., Lonza, AllCells, Allosource), there is often variability in terms of how those cells grow in culture. There are governFull Paper
2017Liu, X;Jiang, Y;Wu, J;Zhang, W;Liang, Y;Jia, L;Yu, J;Jeong, LS;Li, L;NEDD8-activating enzyme inhibitor, MLN4924 (Pevonedistat) induces NOXA-dependent apoptosis through up-regulation of ATF-4Biochemical and biophysical research communications28450112HUVECs (Human umbilical vein endothelial cells) were obtained from Allcells Biotech Co. (Shanghai, China) and maintained in Dulbecco's Modified Eagle's Medium (GIBCO), containing 10% fetal bovine serum (FBS) and 1% Penicillin-Streptomycin solution, aFull Paper
2017Pittala, V;Vanella, L;Salerno, L;Di Giacomo, C;Acquaviva, R;Raffaele, M;Romeo, G;Modica, MN;Prezzavento, O;Sorrenti, V;Novel Caffeic Acid Phenethyl Ester (Cape) Analogues as Inducers of Heme Oxygenase-1Current pharmaceutical design28190391Frozen hMSCs, derived from bone marrow, were purchased from Allcells (Emeryville, CA). After thawing, cells were resuspended in α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 20% heat inactivated fetal bovine serum (FBFull Paper
2017Chan, RC;Kotner, JS;Chuang, CM;Gaur, A;Stabilization of pre-optimized multicolor antibody cocktails for flow cytometry applicationsCytometry. Part B, Clinical cytometry27001933RPMI 1640 medium (Hyclone), containing 10% fetal bovine serum (FBS), 2 mmol/L L-glutamine, and antibiotics. PBMCs used in long-term studies were stored in liquid nitrogen vapor. For some experiments, diseased and normal bone marrow samples were purchFull Paper
2017Dubey, N;Rajan, SS;Bello, YD;Min, KS;Rosa, V;Graphene Nanosheets to Improve Physico-Mechanical Properties of Bioactive Calcium Silicate CementsMaterials (Basel, Switzerland)28772959tion, and Bioactivity The use of human monoradicular premolars and dental pulp stem cells (DPSC) for research was approved by the Institutional Review Board/NUS (Approval Number: NUS 2094). The DPSC (Allcells, Alameda, CA, USA) between passage 3 andFull Paper
2017Seyfried, TF;Gruber, M;Pawlik, MT;Kasper, S;Mandle, RJ;Hansen, E;A new approach for fat removal in a discontinuous autotransfusion device-concept and evaluationVox sanguinis28960338Background Fat present during blood salvage in orthopaedic or cardiac surgery can pose a risk of fat embolism and should be eliminated before transfusion. Based on observations of SaveFull Paper
2017Fei, T;Shao, Y;Yan, Z;Zhu, L;Li, S;Pan, J;Guo, C;The effects of P-gp and CYP450 modulated by rifampicin on the steroid-induced osteonecrosis of the femoral headJournal of bone and mineral metabolism27848009MSCs (RBM-006, ALLCELLS) were added to 24-well plates and cultured for three passages. The MSCs were incubated with blank medium, medium supplemented with dexamethasone (10 μmol/l), and dexamethasone (10 μmol/l) plus rifampicin (5 μg/ml), each treatmFull Paper
2017Mao, M;Zheng, X;Jin, B;Zhang, F;Zhu, L;Cui, L;Effects of CD44 and E-cadherin overexpression on the proliferation, adhesion and invasion of ovarian cancer cellsExperimental and therapeutic medicine29285092CD44 is a prognostic indicator of shorter survival time in ovarian cancer. E‑cadherin fragmentation promotes the progression of ovarian cancer. However, the effects of CD44 and E‑ SaveFull Paper
2017Sutou, S;The 10th anniversary of the publication of genes and environment: memoir of establishing the Japanese environmental mutagen society and a proposal for a new collaborative study on mutagenic hormesisGenes and environment : the official journal of the Japanese Environmental Mutagen Society28265305Some chemicals probably are mutagenic or carcinogenic to virtually allcells of all organisms. Among these would be the unstable chemical species such as alkylating agents and other SaveFull Paper
2017He, L;Fan, F;Hou, X;Gao, C;Meng, L;Meng, S;Huang, S;Wu, H;Resveratrol suppresses pulmonary tumor metastasis by inhibiting platelet-mediated angiogenic responsesThe Journal of surgical research28629815Primary HUVECs were obtained from Allcells (Shanghai, China) and cultured in endothelial basal medium (Allcells) according to the manufacturer's protocol. SaveFull Paper
2017Iwasa, M;Miura, Y;Fujishiro, A;Fujii, S;Sugino, N;Yoshioka, S;Yokota, A;Hishita, T;Hirai, H;Andoh, A;Ichinohe, T;Maekawa, T;Bortezomib interferes with adhesion of B cell precursor acute lymphoblastic leukemia cells through SPARC up-regulation in human bone marrow mesenchymal stromal/stem cellsInternational journal of hematology28044259Human BM-MSCs were isolated from BM samples of healthy adults purchased from AllCells (Emeryville, CA, USA) based on our previously published method. A single-cell suspension SaveFull Paper
2017Suknuntha, K;Thita, T;Togarrati, PP;Ratanachamnong, P;Wongtrakoongate, P;Srihirun, S;Slukvin, I;Hongeng, S;Wnt signaling inhibitor FH535 selectively inhibits cell proliferation and potentiates imatinib-induced apoptosis in myeloid leukemia cell linesInternational journal of hematology27766528Mononuclear cells from CML patient in chronic phase were procured from AllCells (CA, USA). Histopaque-1077 (Sigma-Aldrich) density gradient centrifugation was performed on total SaveFull Paper
2017Hsieh, YT;Aggarwal, P;Cirelli, D;Gu, L;Surowy, T;Mozier, NM;Characterization of FcγRIIIA effector cells used in in vitro ADCC bioassay: Comparison of primary NK cells with engineered NK-92 and Jurkat T cellsJournal of immunological methods27939300HemaCare (HemaCare Corporation, Van Nuys, CA) and AllCells (AllCells, LLC, Alameda, CA), respectively. Engineered NK-92 (V-158) and NK-92 (F-158) effector cell lines were purchased under a license agreement from Conkwest. The parent cells were transfFull Paper
2017Trang, K;Raposo, RA;Lowe, MM;Krow-Lucal, ER;Yonemoto, W;Cabido, VD;SenGupta, D;McCune, JM;Relative mRNA Expression Levels of Restriction Factors and Antiviral Genes in Fetal and Adult Human Monocytes and Monocyte-Derived MacrophagesViral immunology28151065ABM samples were obtained from healthy donors (AllCells, LLC or Lonza, Inc.). Both adult and fetal mononuclear cells were isolated by density centrifugation of a Ficoll-Hypaque SaveFull Paper
2017Zhou, X;Liang, L;Zhao, Y;Zhang, H;Epigallocatechin-3-Gallate Ameliorates Angiotensin II-Induced Oxidative Stress and Apoptosis in Human Umbilical Vein Endothelial Cells through the Activation of Nrf2/Caspase-3 SignalingJournal of vascular research28942440HUVECs were obtained from Allcells Technology, China. The cells were seeded in RPMI 1640 medium with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 60 μg/mL of endothelial cell growth supplement (BD, San Diego, CA, USA) in an incubator atFull Paper
2017Goto, K;Goto, M;Ando-Imaoka, M;Kai, K;Mori, K;Evaluation of drug-induced hematotoxicity using novel in vitro monkey CFU-GM and BFU-E colony assaysThe Journal of toxicological sciences28717098Commercial leukopaks generally contain 80-200 mL of processed material with approximately 7x109peripheral blood mononuclear cells (PBMC), (more information available on: www.allcells.com/products/whole-tissue/leuko-pak). The Table I shows the percentFull Paper
2017Gao, X;Yourick, JJ;Sprando, RL;Comparative transcriptomic analysis of endothelial progenitor cells derived from umbilical cord blood and adult peripheral blood: Implications for the generation of induced pluripotent stem cellsStem cell research29156376Frozen human MNCs from umbilical CB and adult PB of healthy individuals were obtained from AllCells (Alameda, CA). The cells were thawed, washed, and resuspended in EGM-2 SaveFull Paper
2017Enezei, H;Ahmad, A;Khamis, M;Suzuki, J;Sugita, Y;Maeda, H;Alshehadat, S;Razak, N;Abbas, S;Qabbani, A;Alam, M;Enhanced Osteogenic and Angiogenic-Related Gene Expression of Human Dental Stem Cells on Biphasic Calcium Phosphate Scaffold Treated with Vascular Endothelial Growth Factor: Part IJournal of Hard Tissue BiologyCell culture: Commercially obtained DSCs from (AllCells, LLC Company, Emeryville, CA, USA) were cultured in the Dulbecco's Modifi ed Eagle Medium (DMEM) high glucose, (4.5g/l), Fetal bovine serum (FBS), Dulbecco's PBS (without Ca+2 and Mg+2), PenicilFull Paper
2017Jasińska-Stroschein, M;Orszulak-Michalak, D;Novel strategies for treatment of pulmonary arterial hypertensionPostepy higieny i medycyny doswiadczalnej (Online)287919522.3.2 EARLY OUTGROWTH EPC ISOLATION Human early EPCs were generated from leukapheresis samples obtained from Leuko-Paks purchased from AllCells, inc (Almeda, California, USA) or obtained from healthy donors in accordance with the Ottawa Hospital ReseFull Paper
2017Cheng, Z;Yi, Y;Xie, S;Yu, H;Peng, H;Zhang, G;The effect of the JAK2 inhibitor TG101209 against T cell acute lymphoblastic leukemia (T-ALL) is mediated by inhibition of JAK-STAT signaling and activation of the crosstalk between apoptosis and autophagy signalingOncotarget29290986(A) HSD2, peer and bone marrow primary T-ALLcells were treated with increasing concentrations of TG101209 for 48 h, which was followed by analysis of apoptosis by staining with PI SaveFull Paper
2017Chen, C;Li, H;Jiang, J;Zhang, Q;Yan, F;Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD ratsOncotarget29069818t have structural similarities to the human body to obtain more precise results. MATERIALS AND METHODS Cultivation of BMMSCs Bone marrow mesenchymal stem cell (BMMSCs) of SD rats were purchased from AllCells, LLC (Alameda, CA, USA), isolated and purFull Paper
2017Brin, E;Wu, K;Lu, HT;He, Y;Dai, Z;He, W;PEGylated arginine deiminase can modulate tumor immune microenvironment by affecting immune checkpoint expression, decreasing regulatory T cell accumulation and inducing tumor T cell infiltrationOncotarget28938609richia coli. The recombinant protein is PEGylated with 20,000 MW PEG [40-42]. C397A substitution was used to generate the inactive ADI-PEG 20 mutant. Frozen healthy donor PBMCs were purchased from AllCells. Cancer cell lines AGS, A2780, A375, HCT116Full Paper
2017Horn, LA;Ciavattone, NG;Atkinson, R;Woldergerima, N;Wolf, J;Clements, VK;Sinha, P;Poudel, M;Ostrand-Rosenberg, S;CD3xPDL1 bi-specific T cell engager (BiTE) simultaneously activates T cells and NKT cells, kills PDL1+ tumor cells, and extends the survival of tumor-bearing humanized miceOncotarget28938530PBMC used for NKT purification were obtained from AllCells, LLC (Alameda, CA) and were handled according to the manufacturer's direction. Fresh samples of blood from SCLC and SaveFull Paper
2017Bilger, A;Plowshay, J;Ma, S;Nawandar, D;Barlow, EA;Romero-Masters, JC;Bristol, JA;Li, Z;Tsai, MH;Delecluse, HJ;Kenney, SC;Leflunomide/teriflunomide inhibit Epstein-Barr virus (EBV)- induced lymphoproliferative disease and lytic viral replicationOncotarget28574826id cell line (LCL) derived from the peripheral blood leukocytes of anonymous donors, obtained from the American Red Cross [55]. M81-Luc LCLs were obtained by infecting CD34-depleted human cord blood (AllCells) with M81-Luc virus, described below, andFull Paper
2017Estupina, P;Fontayne, A;Barret, JM;Kersual, N;Dubreuil, O;Le Blay, M;Pichard, A;Jarlier, M;Pugnière, M;Chauvin, M;Chardès, T;Pouget, JP;Deshayes, E;Rossignol, A;Abache, T;de Romeuf, C;Terrier, A;Verhaeghe, L;Gaucher, C;Prost, JF;Pèlegrin, A;Navarro-Teulon, I;The anti-tumor efficacy of 3C23K, a glyco-engineered humanized anti-MISRII antibody, in an ovarian cancer model is mainly mediated by engagement of immune effector cellsOncotarget28427157Ficoll procedure. Human and cynomolgus monkey PBMC were frozen in FBS with 10% DMSO and stored in liquid nitrogen until use. Murine PBMC isolated from C57BL/6 mice were directly purchased frozen from AllCells (Alameda, CA, USA). 51Cr-labeled COV434-MFull Paper
2017Diaz, MF;Evans, SM;Olson, SD;Cox, CS;Wenzel, PL;A Co-culture Assay to Determine Efficacy of TNF-α Suppression by Biomechanically Induced Human Bone Marrow Mesenchymal Stem CellsBio-protocol28932763Human bone marrow (BM) MSC (Whole Bone Marrow aspirates) (AllCells, catalog number: ABM001-0) MSCs were isolated from whole bone marrow using a Ficoll gradient followed by plastic adherence and then cultured in MSC media (see Recipes)Full Paper
2017Noorani, TY;Luddin, N;Rahman, IA;Masudi, SM;In Vitro Cytotoxicity Evaluation of Novel Nano-Hydroxyapatite-Silica Incorporated Glass Ionomer CementJournal of clinical and diagnostic research : JCDR28571275Mesenchymal stem cell (MSC) Basal Medium and supplements were purchased from ALLCELLS (USA). Tryp LE express was purchased from Invitrogen (California, USA). For MTT test, SaveFull Paper
2017Wang, J;Zhang, Y;Zhu, Q;Liu, Y;Cheng, H;Zhang, Y;Li, T;Data on the radioprotective effect of emodin in vivo and vitro via inhibition of apoptosis and modulation of p53Data in brief28275662aterials and methods 2.1 Experimental design and materials Male C57BL/6 mice were purchased from SIPPR-BK Experiment Animal Co. (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) from ALLCells, LLC (Emeryville, CA, USA). Emodin (puritFull Paper
2017Ahmed, H;Luddin, N;Kannan, T;Mokhtar, K;Ahmad, A;Dentinogenic differentiation potential of fast set white portland cements of a different origin on dental pulp stem cellsEuropean Journal of General Dentistrymolars (adult) and characterized was purchased from AllCells (MD, USA) and was used in (AllCells), with 50 ml of human mesenchymal stem cell stimulatory supplements (AllCells). SaveFull Paper
2017张文博, ;张贤, ;Influence of M2 macrophage supernatant combined with Eucommia flavonoids on the biological behavior of osteoblasts under hypoxic conditionsChinese Journal of Tissue Engineering Research: 文 快速阅读: 文题释义: M2 型巨噬细胞: 即替代活化巨噬细胞, 由白细胞介 4, 白细胞介 13 诱导, 可使甘露糖受体及转谷氨酰胺酶2 表达上调. M2 型巨噬细胞及其分泌 子可以促进组织 Save;METHODS:AfterMC3T3E1cellswereresuscitated,andM2macrophageswereisolated,inducedandcultured,allcells weredividedintocontrol,M2macrophage, SaveFull Paper
2017Richardson, C;Mechanistic and Therapeutic Studies Related to Anemia of Chronic Disease and InflammationUniversity of Virginiafollows: IFNγ 1500U/ml, TNFα 100ng/ml, IL-1β 100ng/ml, IL-6 100ng/ml IL-10 100ng/ml, IL-15 100ng/ml, and LPS 50ug/ml (PeproTech). Purified human CD36+ cells (AllCells LLC, Emeryville, CA) underwent expansion as previously described [73] for 48 hoursFull Paper
2017Alvey, C;Engineered Marrow Macrophages For Cancer Therapy: Engorgement, Accumulation, Differentiation, And Acquired ImmunityThesisHuman Specimens: Fresh human bone marrow was purchased from AllCells (Cat#: ABM001-1) in 3 mL quantities from healthy donors. AllCells received IRB approval from Alpha (Protocol #7000-SOP-046) which includes donor consent. In total, there were 4 diffFull Paper
2017Walters, MC;Hongeng, S;Kwiatkowski, JL;Locatelli, F;Results from the Hgb-207 (Northstar-2) Trial: A Phase 3 Study to Evaluate Safety and Efficacy of Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia (TDT) in Patients with Non-β0/β0 GenotypesBloodoad : Download full-size image [https://ars.els-cdn.com/content/image/1-s2.0-S000649711981042X-gr1.jpg] DISCLOSURES WALTERS: _bluebird bio:_ Research Funding; _Sangamo Therapeutics:_ Consultancy; _AllCells, Inc:_ Other: Medical Director; _ViaCordFull Paper
2017Talano, JA;Moore, TB;Keever-Taylor, CA;Shenoy, S;Promising Results at 1 Year Follow-up Following Familial Haploidentical (FHI) T-Cell Depleted (TCD) with CD34 Enrichment and T-Cell (CD3) Addback Allogeneic Stem Cell Transplantation in Patients with High-Risk Sickle Cell Disease (SCD) (IND 14359)Bloodls-cdn.com/content/image/1-s2.0-S0006497119851182-gr1_lrg.jpg] * Download : Download full-size image [https://ars.els-cdn.com/content/image/1-s2.0-S0006497119851182-gr1.jpg] DISCLOSURES WALTERS: _AllCells, Inc:_ Other: Medical Director; _bluebirdFull Paper
2017Acharya, S;Defining Immune Cell Profiles and their Role in Predicting Therapeutic Response in Multiple MyelomaThesisDana-Farber Cancer Institute. These samples were collected after informed consent in accordance with the Declaration of Helsinki and approved by the institutional review board of the Dana-Farber Cancer Institute. Healthy donor samples were obtained fFull Paper
2017Accomando, WP;Wiencke, JK;Houseman, EA;HousemanEugenePHHSQuantitativeReconstructionLeukocyte. pdfGenome Biologyblood, that were utilized in this work. Blood was obtained through an institutional review board-approved donor program at AllCells, LLC (Alameda, CA, USA) and research was carried out in accordance with the Helsinki declaration. Samples were de-idenFull Paper
2017Zhang, Y;Farhangfar, F;Giblin, L;Wolf, D;Vitelli, F;Assessment of Differentiation States of Hematopoietic Stem Cells Following in Vitro Culture Using Side and Forward Scatter of Flow CytometryWorld Journal of Research and ReviewG-CSF mobilized peripheral HSC (AllCells, LLC. Emeryville, CA) were cultured with CellGro®Stem Cell Expansion Media (CellGenix, Inc., Catalog number: 2001) containing 10ng/ml TPO (Peprotech, Inc. Rocky Hill, NJ. Catalog number: 300-18), 50ng/ml SCFFull Paper
2017Gregg, KA;Novel Lipid A Structures for Adjuvant Discovery and DevelopmentThesis100 IU/mL penicillin, 100 µg/mL streptomycin, 1 mM sodium pyruvate, and 200 mM l-glutamine. THP-1 (ATCC, Manassas, VA), primary mouse splenocytes, and primary human PBMCs (AllCells, LLC, Alameda, CA) were cultured in RPMI 1640Full Paper
2017Edun, FA;Endothelial cell response to bioactive peptides and stiffened extracellular matrices: an investigation into atherogenic pathwaysThesisexample, samples delivered from AllCells (California, U.S.A.) had markedly different culture adherence and proliferation characteristics (cell doubling time) than HUVECs obtained from ThermoFisher, prompting our repeated purchase of only batch numberFull Paper
2017Bonar, MM;Rapid Enumeration, Sorting and Maturation Analysis of Single Viral Particles in HIV-1 Swarms by High-resolution Flow VirometryThesisidentified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. CD4+ T cells were isolated by adding additional autologous red blood cells to leukapheresis samples and RosetteSep CD4+ T cell enrichment kFull Paper
2017Basiorka, AA;Changing the pathobiological paradigm in myelodysplastic syndromes: The NLRP3 inflammasome drives the MDS phenotypeThesisNormal, heparinized BM aspirates were purchased from Lonza Walkersville or AllCells, LLC. Normal and MDS BM mononuclear cells (BMMNC) were isolated from heparinized BM aspirates using Ficoll-Hypaque Plus gradient centrifugation (GE Healthcare). RecomFull Paper
2017Bennett, LF;Analysis of Stress Erythropoiesis during Inflammation: Stimulation of Toll-Like Receptors Induces Erythrophagocytosis and Activates Stress ErythropoiesisThesisAfter 24 hours, cells were washed and replated with fresh SEEM. Human bone marrow mononuclear cells were purchased from ReachBio (0300-200) and cultured in SEEM containing human insulin, human holo-transferrin, and human cytokines. Human MNCs cultureFull Paper
2017Newkirk, SJ;Lee, S;Grandi, FC;Gaysinskaya, V;Rosser, JM;Vanden Berg, N;Hogarth, CA;Marchetto, MCN;Muotri, AR;Griswold, MD;Ye, P;Bortvin, A;Gage, FH;Boeke, JD;An, W;Intact piRNA pathway prevents L1 mobilization in male meiosisProceedings of the National Academy of Sciences of the United States of America28630288wrote the paper. Reviewers: P.D., Tulane Cancer Center; and P.J.W., University of Pennsylvania. 1Present address: Cancer Biology Program, Stanford University, Stanford, CA 94305. 2Present address: ReachBio LLC, Seattle, WA 98107. 3Present addressFull Paper
2017Zhen, A;Peterson, CW;Carrillo, MA;Reddy, SS;Youn, CS;Lam, BB;Chang, NY;Martin, HA;Rick, JW;Kim, J;Neel, NC;Rezek, VK;Kamata, M;Chen, ISY;Zack, JA;Kiem, HP;Kitchen, SG;Long-term persistence and function of hematopoietic stem cell-derived chimeric antigen receptor T cells in a nonhuman primate model of HIV/AIDSPLoS pathogens29284044Following conditioning, the HSPC product was infused back into the autologous animal. A small aliquot of the infused cell product was plated in Colony Gel Medium (Reach Bio, Seattle, WA) and analyzed as previously described[14,39]. Individual colonieFull Paper
2017Liu, FD;Pishesha, N;Poon, Z;Kaushik, T;Van Vliet, KJ;Material Viscoelastic Properties Modulate the Mesenchymal Stem Cell Secretome for Applications in Hematopoietic RecoveryACS biomaterials science & engineering33445371Human bone marrow-derived MSCs (MSCs) were purchased from multiple commercial sources (Lonza, RoosterBio, and ReachBio) at what was stated by the vendors to be a low SaveFull Paper
2017Hinerfeld, D;Fuhrman, K;Demirkan, G;DosSantos, G;Geiss, G;A simple and integrated workflow for deep proteomic and transcriptomic analysis of sorted cell populationsThe Journal of Immunology1Nanostring Technologies++1Nanostring Technologies++1Nanostring Technologies++2Reachbio Research Labs++1Nanostring TechnologiesFull Paper
2017Min, C;Moore, N;Shearstone, JR;Quayle, SN;Huang, P;van Duzer, JH;Jarpe, MB;Jones, SS;Yang, M;Selective Inhibitors of Histone Deacetylases 1 and 2 Synergize with Azacitidine in Acute Myeloid LeukemiaPloS one28060870Specifically, compounds were dissolved in DMSO and added to tubes of methylcellulose-based medium (Reachbio, catalog 1103) containing cytokine formulations to support colony Save;row samples from AML patients were treated with ACY-957 alone, azacitFull Paper
2017Kudrimoti, M;Curtis, A;Azawi, S;Worden, F;Katz, S;Adkins, D;Bonomi, M;Scott, Z;Elder, J;Sonis, ST;Straube, R;Donini, O;Dusquetide: Reduction in oral mucositis associated with enduring ancillary benefits in tumor resolution and decreased mortality in head and neck cancer patientsBiotechnology reports (Amsterdam, Netherlands)286495571).Fig. 1Dusquetide Pre-Incubated with Stromal Cells inhibits the Future Growth of Co-cultured Multiple Myeloma Cells. Previously defined primary human bone marrow stromal (mesenchymal (MSC)) cells (ReachBio lot# 2221207) were plated at a concentratiFull Paper
2017Bennett, LF;Analysis of Stress Erythropoiesis: Stimulation of Toll-Like Receptors Induces Erythrophagocytosis and Activates Stress ErythropoiesisThesisAfter 24 hours, cells were washed and replated with fresh SEEM. Human bone marrow mononuclear cells were purchased from ReachBio (0300-200) and cultured in SEEM containing human insulin, human holo-transferrin, and human cytokines. Human MNCs cultureFull Paper
2017Bogeholz, JL;CD 33 in der akuten melodischen LeukämieThesisColonyGel 1101 ReachBio, Seattle, WA Steriles Wasser Helmholtzzentrum Fc-Block (FcR Blocking Reagent) Miltenyi Biotec, Bergisch GladbachFull Paper
2016Goldberg, SB;Gettinger, SN;Mahajan, A;Chiang, AC;Herbst, RS;Sznol, M;Tsiouris, AJ;Cohen, J;Vortmeyer, A;Jilaveanu, L;Yu, J;Hegde, U;Speaker, S;Madura, M;Ralabate, A;Rivera, A;Rowen, E;Gerrish, H;Yao, X;Chiang, V;Kluger, HM;Pembrolizumab for patients with melanoma or non-small-cell lung cancer and untreated brain metastases: early analysis of a non-randomised, open-label, phase 2 trialLancet Oncol.27267608In patients with NSCLC, PD-L1 staining was done by Qualtek (Goleta, CA, USA) on tissue from any disease site after the most recent systemic therapy employing previously described methods 6 using 1% staining as a cutpoint for positivityFull Paper
2016Seiwert, TY;Burtness, B;Mehra, R;Weiss, J;Berger, R;Eder, JP;Heath, K;McClanahan, T;Lunceford, J;Gause, C;Cheng, JD;Chow, LQ;Safety and clinical activity of pembrolizumab for treatment of recurrent or metastatic squamous cell carcinoma of the head and neck (KEYNOTE-012): an open-label, multicentre, phase 1b trialLancet Oncol.27247226Funding for this research was provided by Merck & Co, Inc, Kenilworth, NJ, USA. The authors thank the patients and their families and caregivers for participating in the study. The authors also thank Mark Ayers, Michael Nebozhyn, Erin Murphy, and JenFull Paper
2016Muro, K;Chung, HC;Shankaran, V;Geva, R;Catenacci, D;Gupta, S;Eder, JP;Golan, T;Le, DT;Burtness, B;McRee, AJ;Lin, CC;Pathiraja, K;Lunceford, J;Emancipator, K;Juco, J;Koshiji, M;Bang, YJ;Pembrolizumab for patients with PD-L1-positive advanced gastric cancer (KEYNOTE-012): a multicentre, open-label, phase 1b trialLancet Oncol.27157491To screen for PD-L1 expression at enrolment, archival tumour samples were assessed with a laboratory-developed prototype immunohistochemistry assay (QualTek Molecular Laboratories, Goleta, CA, USA), which included the 22C3 antibody (Merck & Co, KenilFull Paper
2016Snyder, MW;Kircher, M;Hill, AJ;Daza, RM;Shendure, J;Cell-free DNA Comprises an In Vivo Nucleosome Footprint that Informs Its Tissues-Of-OriginCell26771485... man peripheral blood plasma from healthy donors, donors with clinical diagnosis of Stage IV cancer, and donors with clinical diagnosis with autoimmune disease (Tables S1, S4 and S5) was obtained from Conversant Bio (Huntsville, Alabama, USA) or PFull Paper
2016Armand, P;Shipp, MA;Ribrag, V;Michot, JM;Zinzani, PL;Kuruvilla, J;Snyder, ES;Ricart, AD;Balakumaran, A;Rose, S;Moskowitz, CH;Programmed Death-1 Blockade With Pembrolizumab in Patients With Classical Hodgkin Lymphoma After Brentuximab Vedotin FailureJ. Clin. Oncol.27354476PD-L1 expression was determined using either fresh or archival formalin-fixed, paraffin-embedded tissue sectioned at 4 to 5 microns, with a proprietary assay developed at QualTek Molecular Laboratories (Newtown, PA) in collaboration with Merck (KenilFull Paper
2016Nanda, R;Chow, LQ;Dees, EC;Berger, R;Gupta, S;Geva, R;Pusztai, L;Pathiraja, K;Aktan, G;Cheng, JD;Karantza, V;Buisseret, L;Pembrolizumab in Patients With Advanced Triple-Negative Breast Cancer: Phase Ib KEYNOTE-012 StudyJ. Clin. Oncol.27138582Filhart and Kenneth Emancipator (Merck & Co., Kenilworth, NJ) for immunohistochemistry expertise and study support, Roger Dansey (Merck & Co.) for critical manuscript review and study support, Karl Heath (Merck & Co.) for study support, and QualTek MFull Paper
2016Imsland, F;McGowan, K;Rubin, CJ;Henegar, C;Sundström, E;Berglund, J;Schwochow, D;Gustafson, U;Imsland, P;Lindblad-Toh, K;Lindgren, G;Mikko, S;Millon, L;Wade, C;Schubert, M;Orlando, L;Penedo, MC;Barsh, GS;Andersson, L;Regulatory mutations in TBX3 disrupt asymmetric hair pigmentation that underlies Dun camouflage color in horsesNat. Genet.26691985Science for Life Laboratory Uppsala, Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, USA.++Science for Life Laboratory Uppsala, Department of MedFull Paper
2016Wang, G;Lu, X;Dey, P;Deng, P;Wu, CC;Jiang, S;Fang, Z;Zhao, K;Konaparthi, R;Hua, S;Zhang, J;Li-Ning-Tapia, EM;Kapoor, A;Wu, CJ;Patel, NB;Guo, Z;Ramamoorthy, V;Tieu, TN;Heffernan, T;Zhao, D;Shang, X;Khadka, S;Hou, P;Hu, B;Jin, EJ;Yao, W;Pan, X;Ding, Z;Shi, Y;Li, L;Chang, Q;Troncoso, P;Logothetis, CJ;McArthur, MJ;Chin, L;Wang, YA;DePinho, RA;Targeting YAP-Dependent MDSC Infiltration Impairs Tumor ProgressionCancer Discov26701088... d R&D Biosystems (Minneaplois, MN). Cxcr2 antibody was obtained from Bioss and R & D Biosystems. CD45 and Ly-6G antibodies were obtained from Biolegend. Prostate tissue micrcroarray was obtained from Folio Bioscience. CHROMATIN IMMUNOPRECIPITATIFull Paper
2016Vilgelm, AE;Johnson, CA;Prasad, N;Yang, J;Chen, SC;Ayers, GD;Pawlikowski, JS;Raman, D;Sosman, JA;Kelley, M;Ecsedy, JA;Shyr, Y;Levy, SE;Richmond, A;Connecting the Dots: Therapy-Induced Senescence and a Tumor-Suppressive Immune MicroenvironmentJ. Natl. Cancer Inst.26719346Tennessee Valley Healthcare System, Department of Veterans Affairs, Nashville, TN (AEV, CAJ, JY, JSP, DR, AR); Department of Cancer Biology (AEV, AJ, JY, SCC, JSP, DR, AR), Center for Quantitative Sciences (SCC), Division of Cancer Biostatistics, DepFull Paper
2016Sun, JM;Zhou, W;Choi, YL;Choi, SJ;Kim, SE;Wang, Z;Dolled-Filhart, M;Emancipator, K;Wu, D;Weiner, R;Frisman, D;Kim, HK;Choi, YS;Shim, YM;Kim, J;Prognostic Significance of PD-L1 in Patients with Non-Small Cell Lung Cancer: A Large Cohort Study of Surgically Resected CasesJ Thorac Oncol27103510Automated staining (QualTek TekMate 500, QualTek Molecular Laboratories, Newtown, PA) included rinses with 1× wash buffer (#K8012 Envision FLEX+ kit, Dako), 10-minute proteinase K incubation (#S3020, Dako), 1× wash buffer rinse, and peroxidase blockiFull Paper
2016Wakeman, CA;Moore, JL;Noto, MJ;Zhang, Y;Singleton, MD;Prentice, BM;Gilston, BA;Doster, RS;Gaddy, JA;Chazin, WJ;Caprioli, RM;Skaar, EP;The innate immune protein calprotectin promotes Pseudomonas aeruginosa and Staphylococcus aureus interactionNat Commun27301800Total RNA was harvested using a combination of LETS buffer (0.1 M LiCl; 10 mM EDTA; 10 mM Tris HCl pH 7.4; 1% SDS) and TRI Reagent as previously described61. qRT–PCR was performed using SYBR green supermix (Bio-Rad) following manufacturer’s instructiFull Paper
2016Yang, Z;Shah, K;Khodadadi-Jamayran, A;Jiang, H;Dpy30 is critical for maintaining the identity and function of adult hematopoietic stem cellsJ. Exp. Med.27647347Total RNAs and ChIP (and their corresponding input) DNAs were sent to the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology (Huntsville, AL) for sequencing, including library construction. In brief, the quality of the total RNA andFull Paper
2016Resendez, SL;Jennings, JH;Ung, RL;Namboodiri, VM;Zhou, ZC;Otis, JM;Nomura, H;McHenry, JA;Kosyk, O;Stuber, GD;Visualization of cortical, subcortical and deep brain neural circuit dynamics during naturalistic mammalian behavior with head-mounted microscopes and chronically implanted lensesNat Protoc26914316Dust-off compressed gas (Falcon Safety Products, DPSXL) Gelfoam absorbable sponge (Pfizer Injectables, cat. no. 00009-0342-01) 12-well culture plates (Falcon, cat. no. 353043) Skull screws (Glass and Watch Screws, 900 piece set, JT69900) 1/16-incFull Paper
2016Amendola, LM;Jarvik, GP;Leo, MC;McLaughlin, HM;Akkari, Y;Amaral, MD;Berg, JS;Biswas, S;Bowling, KM;Conlin, LK;Cooper, GM;Dorschner, MO;Dulik, MC;Ghazani, AA;Ghosh, R;Green, RC;Hart, R;Horton, C;Johnston, JJ;Lebo, MS;Milosavljevic, A;Ou, J;Pak, CM;Patel, RY;Punj, S;Richards, CS;Salama, J;Strande, NT;Yang, Y;Plon, SE;Biesecker, LG;Rehm, HL;Performance of ACMG-AMP Variant-Interpretation Guidelines among Nine Laboratories in the Clinical Sequencing Exploratory Research ConsortiumAm. J. Hum. Genet.27181684Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA.++Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195, USA. Electronic address: pair@u.washington.eduFull Paper
2016Green, RC;Goddard, KAB;Jarvik, GP;Amendola, LM;Appelbaum, PS;Berg, JS;Bernhardt, BA;Biesecker, LG;Biswas, S;Blout, CL;Bowling, KM;Brothers, KB;Burke, W;Caga-Anan, CF;Chinnaiyan, AM;Chung, WK;Clayton, EW;Cooper, GM;East, K;Evans, JP;Fullerton, SM;Garraway, LA;Garrett, JR;Gray, SW;Henderson, GE;Hindorff, LA;Holm, IA;Lewis, MH;Hutter, CM;Janne, PA;Joffe, S;Kaufman, D;Knoppers, BM;Koenig, BA;Krantz, ID;Manolio, TA;McCullough, L;McEwen, J;McGuire, A;Muzny, D;Myers, RM;Nickerson, DA;Ou, J;Parsons, DW;Petersen, GM;Plon, SE;Rehm, HL;Roberts, JS;Robinson, D;Salama, JS;Scollon, S;Sharp, RR;Shirts, B;Spinner, NB;Tabor, HK;Tarczy-Hornoch, P;Veenstra, DL;Wagle, N;Weck, K;Wilfond, BS;Wilhelmsen, K;Wolf, SM;Wynn, J;Yu, JH;, ;Clinical Sequencing Exploratory Research Consortium: Accelerating Evidence-Based Practice of Genomic MedicineAm. J. Hum. Genet.27181682Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Harvard Medical School, Boston, MA 02115, USA; Partners Personalized Medicine, Boston, MAFull Paper
2016Lieberman, LA;Zeng, W;Singh, C;Wang, W;Otipoby, KL;Loh, C;Plavina, T;Gorelik, L;Ransohoff, RM;Cahir-McFarland, E;CD62L is not a reliable biomarker for predicting PML risk in natalizumab-treated R-MS patientsNeurology26718566Thirty-four frozen PBMC samples were purchased from Conversant Bio (Huntsville, AL) to test the effects of other medical conditions on the % CD62L determination. Twenty-one matched healthy donor samples were collected. Eight samples were collected frFull Paper
2016Hu, X;Liu, X;Moisan, J;Wang, Y;Lesch, CA;Spooner, C;Morgan, RW;Zawidzka, EM;Mertz, D;Bousley, D;Majchrzak, K;Kryczek, I;Taylor, C;Van Huis, C;Skalitzky, D;Hurd, A;Aicher, TD;Toogood, PL;Glick, GD;Paulos, CM;Zou, W;Carter, LL;Synthetic RORγ agonists regulate multiple pathways to enhance antitumor immunityOncoimmunology28123897... ion. After 5 days, cytokine levels in the media were determined using a luminex panel (R&D Systems). Cells were collected for flow cytometry analysis. PBMCs from cancer patients were purchased from Conversant Bio and activated with anti-CD3/28 bFull Paper
2016Cataisson, C;Michalowski, AM;Shibuya, K;Ryscavage, A;Klosterman, M;Wright, L;Dubois, W;Liu, F;Zhuang, A;Rodrigues, KB;Hoover, S;Dwyer, J;Simpson, MR;Merlino, G;Yuspa, SH;MET signaling in keratinocytes activates EGFR and initiates squamous carcinogenesisSci Signal27330189This procedure was performed by a commercial vendor (Phylogeny, Inc.) according to the published method (88). FFPE TMAs from US Biomax were deparaffinized for 5 minutes in xylene, immersed in 100% ethanol for 5 minutes then air-dried. Treatment was wFull Paper
2016Bryant, SA;Herdy, JR;Amemiya, CT;Smith, JJ;Characterization of Somatically-Eliminated Genes During Development of the Sea Lamprey (Petromyzon marinus)Mol. Biol. Evol.27288344Total RNA was isolated from lamprey embryos using Trizol extraction. RNA quality was assessed on the Agilent 2100 Bioanalyzer (Agilent Technologies) and samples with RNA Integrity Number (RIN) 8 were sent to the HudsonAlpha Genomic Services Lab (HudsFull Paper
2016Jiang, L;Huang, J;Higgs, BW;Hu, Z;Xiao, Z;Yao, X;Conley, S;Zhong, H;Liu, Z;Brohawn, P;Shen, D;Wu, S;Ge, X;Jiang, Y;Zhao, Y;Lou, Y;Morehouse, C;Zhu, W;Sebastian, Y;Czapiga, M;Oganesyan, V;Fu, H;Niu, Y;Zhang, W;Streicher, K;Tice, D;Zhao, H;Zhu, M;Xu, L;Herbst, R;Su, X;Gu, Y;Li, S;Huang, L;Gu, J;Han, B;Jallal, B;Shen, H;Yao, Y;Genomic Landscape Survey Identifies SRSF1 as a Key Oncodriver in Small Cell Lung CancerPLoS Genet.27093186... s >70%; the tumor content in each NAT was< 3%. The Caucasian SCLC patient cohort consisted of 25 FFPE lung tumor tissue specimens with matched normal adjacent tissue pairs, which were purchased from Conversant Biologics, Inc (Huntsville, AL) (S1Full Paper
2016Oksenberg, D;Dufu, K;Patel, MP;Chuang, C;Li, Z;Xu, Q;Silva-Garcia, A;Zhou, C;Hutchaleelaha, A;Patskovska, L;Patskovsky, Y;Almo, SC;Sinha, U;Metcalf, BW;Archer, DR;GBT440 increases haemoglobin oxygen affinity, reduces sickling and prolongs RBC half-life in a murine model of sickle cell diseaseBr. J. Haematol.27378309USA (IRB 88‐034)]. Blood from healthy donors was obtained from the Stanford Blood Center (Stanford, CA, USA) and umbilical cord blood was obtained from Conversant Bio, Inc. (Huntsville, AL, USA). The anti‐coagulant wasFull Paper
2016Kirby, MK;Ramaker, RC;Gertz, J;Davis, NS;Johnston, BE;Oliver, PG;Sexton, KC;Greeno, EW;Christein, JD;Heslin, MJ;Posey, JA;Grizzle, WE;Vickers, SM;Buchsbaum, DJ;Cooper, SJ;Myers, RM;RNA sequencing of pancreatic adenocarcinoma tumors yields novel expression patterns associated with long-term survival and reveals a role for ANGPTL4Mol Oncol27282075... wling for critical reading of the manuscript. We thank Dr. Nick Cochran for packaging the _ANGPTL4_ lentiviral vectors. We thank Dr. Shawn Levy, Braden Boone, Angela Jones, and all the members of the HudsonAlpha Genomic Services Lab for providingFull Paper
2016O'Shannessy, DJ;Smith, MF;Somers, EB;Jackson, SM;Albone, E;Tomkowicz, B;Cheng, X;Park, Y;Fernando, D;Milinichik, A;Kline, B;Fulton, R;Oberoi, P;Nicolaides, NC;Novel antibody probes for the characterization of endosialin/TEM-1Oncotarget27494870... btained from various commercial vendors with Institutional Review Board approvals and patient consent and were collected between 2009 and 2011 (Bioreclamation, Westbury, NY; Indivumed, Lewisburg, PA; Folio Biosciences, Columbus, OH; Discovery LifFull Paper
2016Ladd, B;Mazzola, AM;Bihani, T;Lai, Z;Bradford, J;Collins, M;Barry, E;Goeppert, AU;Weir, HM;Hearne, K;Renshaw, JG;Mohseni, M;Hurt, E;Jalla, S;Bao, H;Hollingsworth, R;Reimer, C;Zinda, M;Fawell, S;D'Cruz, CM;Effective combination therapies in preclinical endocrine resistant breast cancer models harboring ER mutationsOncotarget27472462... ired ER mutations. MATERIALS AND METHODS Generation of the CTX model/efficacy studies Blood samples from MBC patients, consented according to the Human Biological Samples Policy, were purchased from Conversant Biologics. Due to patient confidentFull Paper
2016Bradford, JR;Wappett, M;Beran, G;Logie, A;Delpuech, O;Brown, H;Boros, J;Camp, NJ;McEwen, R;Mazzola, AM;D'Cruz, C;Barry, ST;Whole transcriptome profiling of patient-derived xenograft models as a tool to identify both tumor and stromal specific biomarkersOncotarget26980748... subcutaneously into either NSG or CB17 SCID mice. The HBXF-079 sample was implanted orthotopically. The CTC-174 model was developed from circulating tumor cells in patient blood samples procured from Conversant Biologics (Huntsville, AL) and consFull Paper
2016Malek, E;Driscoll, JJ;High throughput chemical library screening identifies a novel p110-δ inhibitor that potentiates the anti-myeloma effect of bortezomibOncotarget... om Selleck Chemicals (Houston, TX). Human bone marrow stromal cell line HS-5 (ATCC) was cultured in DMEM. Patient bone marrow aspirates were obtained after Institutional Review Board approval or from ConversantBio (Huntsville, AL) and CD138+ cellFull Paper
2016Aeffner, F;Martin, NT;Peljto, M;Black, JC;Major, JK;Jangani, M;Ports, MO;Krueger, JS;Young, GD;Quantitative assessment of pancreatic cancer precursor lesions in IHC-stained tissue with a tissue image analysis platformLab. Invest.27775692tissue was evaluated. Materials and methods. Human PDAC Tissue. IRB-approved, formalin-fixed tumor samples from pancreatic cancer patients were provided by Conversant Bio (Huntsville, AL, USA). Animals. Kras LSL-G12DFull Paper
2016Haskett, S;Ding, J;Zhang, W;Thai, A;Cullen, P;Xu, S;Petersen, B;Kuznetsov, G;Jandreski, L;Hamann, S;Reynolds, TL;Allaire, N;Zheng, TS;Mingueneau, M;Identification of Novel CD4+ T Cell Subsets in the Target Tissue of Sjögren's Syndrome and Their Differential Regulation by the Lymphotoxin/LIGHT Signaling AxisJ. Immunol.27815440Blocks of formalin-fixed tissue biopsies were acquired from Folio Biosciences (Powell, OH). Salivary tissues included submandibular glands (n = 2), parotid glands (n = 1), and labial glands (n = 3) from Sjo¨gren’s patients. Diagnoses were consistentFull Paper
2016Yoder, KE;Bundschuh, R;Host Double Strand Break Repair Generates HIV-1 Strains Resistant to CRISPR/Cas9Sci Rep27404981HIV-1 infections were continued for 21 days in 4 replicates per cell type and virus strain. The virus supernatant was passaged to uninfected CRISPR cells. Infection was visually confirmed by the presence of syncytia. Two weeks later the genomic DNA wFull Paper
2016Dolled-Filhart, M;Locke, D;Murphy, T;Lynch, F;Yearley, JH;Frisman, D;Pierce, R;Weiner, R;Wu, D;Emancipator, K;Development of a Prototype Immunohistochemistry Assay to Measure Programmed Death Ligand-1 Expression in Tumor TissueArch. Pathol. Lab. Med.27788043Merck & Co, Inc, Kenilworth, New Jersey; the Departments of Early Clinical & Translational Research, Clinical Histochemistry (Dr Locke), Research and Clinical Client Services (Dr Murphy), and Operations and Client Services (Dr Lynch), QualTek MoleculFull Paper
2016Dolled-Filhart, M;Roach, C;Toland, G;Stanforth, D;Jansson, M;Lubiniecki, GM;Ponto, G;Emancipator, K;Development of a Companion Diagnostic for Pembrolizumab in Non-Small Cell Lung Cancer Using Immunohistochemistry for Programmed Death Ligand-1Arch. Pathol. Lab. Med.27552095The prototype PD-L1 IHC assay was used to determine enrollment of patients in KEYNOTE-001 and was performed at a single laboratory site at QualTek (Goleta, California), which is a College of American Pathologists/Clinical Laboratory Improvement AmendFull Paper
2016Durning, SP;Flanagan-Steet, H;Prasad, N;Wells, L;O-Linked β-N-acetylglucosamine (O-GlcNAc) Acts as a Glucose Sensor to Epigenetically Regulate the Insulin Gene in Pancreatic Beta CellsJ. Biol. Chem.26598517samples after the previously described 1-h incubation. Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL) for RNA-Seq library prep and sequencing. Briefly, the concentration and integrity of theFull Paper
2016VonDran, M;Thomas, JA;Freund, MP;Ritsick, M;Orr, M;Kaye, WE;Bakker, A;Knight, P;The National Disease Research Interchange and Collaborators on: What Are the Major Hurdles to the Recovery of Human Tissue to Advance Research?Biopreserv Biobank27845557The Foundation has collected more than 100 cutaneous neurofibromas along with patient-reported NF medical histories. Both tissue and data are stored at Precision Bioservices (Frederick, MD). Full “omic” characterization of 50 samples was outsourced tFull Paper
2016Chisamore, MJ;Gentile, MA;Dillon, GM;Baran, M;Gambone, C;Riley, S;Schmidt, A;Flores, O;Wilkinson, H;Alves, SE;A novel selective androgen receptor modulator (SARM) MK-4541 exerts anti-androgenic activity in the prostate cancer xenograft R-3327G and anabolic activity on skeletal muscle mass & function in castrated miceJ. Steroid Biochem. Mol. Biol.27106747Excised tumor samples were immediately fixed with 10% buffered formalin, and sent to QualTek (Santa Barbara, CA) where tumors were embedded in paraffin, 4-μm thick sections were mounted on charged slides, dried at 60 °C for one hour, and deparaffinzeFull Paper
2016Qadota, H;Mayans, O;Matsunaga, Y;McMurry, JL;Wilson, KJ;Kwon, GE;Stanford, R;Deehan, K;Tinley, TL;Ngwa, VM;Benian, GM;The SH3 domain of UNC-89 (obscurin) interacts with paramyosin, a coiled-coil protein, in Caenorhabditis elegans muscleMol. Biol. Cell27009202Genomic DNA from each strain was prepared and provided to the Emory Integrated Genomics Core (EIGC) for Illumina sequencing. The Genomic Services Laboratory at the HudsonAlpha Institute for Biotechnology (Huntsville, AL) performed quality control andFull Paper
2016Slamecka, J;Salimova, L;McClellan, S;van Kelle, M;Kehl, D;Laurini, J;Cinelli, P;Owen, L;Hoerstrup, SP;Weber, B;Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditionsCell Cycle26654216RNA was extracted from iPSC cultures using RNeasy Plus Mini kit (74134, Qiagen, https://www.qiagen.com/us/shop/sample-technologies/rna-sample-technologies/total-rna/rneasy-plus-micro-and-mini-kits). Whole genome expression analysis was performed by tFull Paper
2016Aycock, KI;Campbell, RL;Lynch, FC;Manning, KB;Craven, BA;The Importance of Hemorheology and Patient Anatomy on the Hemodynamics in the Inferior Vena CavaAnn Biomed Eng27272211Full Paper
2016Rahman, KM;Camp, ME;Prasad, N;McNeel, AK;Levy, SE;Bartol, FF;Bagnell, CA;Age and Nursing Affect the Neonatal Porcine Uterine TranscriptomeBiol. Reprod.26632611United States Department of Agriculture, Agriculture Research Service, Clay Center, Nebraska Department of Animal Sciences, Endocrinology and Animal Biosciences Program, Rutgers University, New Brunswick, New Jersey.++Department of Animal Sciences, EFull Paper
2016Havird, JC;Santos, SR;Developmental Transcriptomics of the Hawaiian Anchialine Shrimp Halocaridina rubra Holthuis, 1963 (Crustacea: Atyidae)Integr. Comp. Biol.27400978RNA fragmentation, first-strand cDNA synthesis and amplification, adapter and barcode addition, and size selection were then performed according to Meyer et al. (2011) using the step-by-step protocol at http://people.oregonstate.edu/∼meyere/tools.htmFull Paper
2016Sorensen, SF;Zhou, W;Dolled-Filhart, M;Georgsen, JB;Wang, Z;Emancipator, K;Wu, D;Busch-Sørensen, M;Meldgaard, P;Hager, H;PD-L1 Expression and Survival among Patients with Advanced Non-Small Cell Lung Cancer Treated with ChemotherapyTransl Oncol26947883gen retrieval, along with a 10-minute proteinase K incubation (#S3020, Dako) following a 1 × wash buffer rinse (#K8012 Envision FLEX + kit, Dako) as part of automated staining. Automated staining (QualTek TekMate 500, Newtown, PA) was followed by perFull Paper
2016Zea, L;Prasad, N;Levy, SE;Stodieck, L;Jones, A;Shrestha, S;Klaus, D;A Molecular Genetic Basis Explaining Altered Bacterial Behavior in SpacePLoS ONE27806055BioServe Space Technologies, Aerospace Engineering Sciences Dept., University of Colorado, Boulder, CO, United States of America.++Genomic Services Laboratory, HudsonAlpha Institute for Biotechnology, Huntsville, AL, United States of America.++GenomiFull Paper
2016McCoy, CR;Rana, S;Stringfellow, SA;Day, JJ;Wyss, JM;Clinton, SM;Kerman, IA;Neonatal maternal separation stress elicits lasting DNA methylation changes in the hippocampus of stress-reactive Wistar Kyoto ratsEur. J. Neurosci.27643783; Samples were sent to HudsonAlpha Genomic Services Laboratory (Huntsville, AL; http://gsl.hudsonalpha.org) for next‐generation sequencing using NEBNext reagents (New England Biolabs, Ipswich, MA) according to manufacturer's recommendationsFull Paper
2016Beck, BH;Fuller, SA;Li, C;Green, BW;Zhao, H;Rawles, SD;Webster, CD;Peatman, E;Hepatic transcriptomic and metabolic responses of hybrid striped bass (Morone saxatilis×Morone chrysops) to acute and chronic hypoxic insultComp. Biochem. Physiol. Part D Genomics Proteomics26851735treatment) were used for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25Full Paper
2016Póvoa, TF;Oliveira, ER;Basílio-de-Oliveira, CA;Nuovo, GJ;Chagas, VL;Salomão, NG;de Barcelos Alves, AM;Mota, EM;Paes, MV;Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing CellsPLoS ONE28006034Janeiro, Brazil. ⨯. Gerard J. Nuovo, Affiliations Ohio State University Comprehensive Cancer Center, Columbus, Ohio, United States of America, Phylogeny Inc, Powell, Ohio, United States of America. ⨯. Vera LA Chagas, AffiliationFull Paper
2016Stavropoulos, SW;Chen, JX;Sing, RF;Elmasri, F;Silver, MJ;Powell, A;Lynch, FC;Abdel Aal, AK;Lansky, A;Muhs, BE;, ;Analysis of the Final DENALI Trial Data: A Prospective, Multicenter Study of the Denali Inferior Vena Cava FilterJ Vasc Interv Radiol27569678Full Paper
2016Gardet, A;Chou, WC;Reynolds, TL;Velez, DB;Fu, K;Czerkowicz, JM;Bajko, J;Ranger, AM;Allaire, N;Kerns, HM;Ryan, S;Legault, HM;Dunstan, RW;Lafyatis, R;Lukashev, M;Viney, JL;Browning, JL;Rabah, D;Pristane-Accelerated Autoimmune Disease in (SWR X NZB) F1 Mice Leads to Prominent Tubulointerstitial Inflammation and Human Lupus Nephritis-Like FibrosisPLoS ONE27760209AZ, USA). Needle biopsies from 5 patients with LN (Class II, 1 patient; Class III, 1 patient; Class IV, 1 patient; Class IV/V, 2 patients) were obtained from Folio Biosciences (Powell, OH) and stained using the same methods. ForFull Paper
2016Shearstone, JR;Golonzhka, O;Chonkar, A;Tamang, D;van Duzer, JH;Jones, SS;Jarpe, MB;Chemical Inhibition of Histone Deacetylases 1 and 2 Induces Fetal Hemoglobin through Activation of GATA2PLoS ONE27073918CS2 differentiation media is same as CS2 expansion media, except 3 U/mL EPO. Peripheral blood from patients homozygous for the sickle cell mutation (Conversant Bio) were collected in heparinized tubes and shipped overnight on cool packsFull Paper
2016Shimizu, T;Seto, T;Hirai, F;Takenoyama, M;Nosaki, K;Tsurutani, J;Kaneda, H;Iwasa, T;Kawakami, H;Noguchi, K;Shimamoto, T;Nakagawa, K;Phase 1 study of pembrolizumab (MK-3475; anti-PD-1 monoclonal antibody) in Japanese patients with advanced solid tumorsInvest New Drugs27000274on the confirmation test. PD-L1 expression. PD-L1 expression was measured using immunohistochemistry performed on formalin-fixed, paraffin-embedded tissue sections at QualTek Clinical Laboratories. PD-L1 positivity was ;or postdose sample was poFull Paper
2016Meaburn, KJ;Agunloye, O;Devine, M;Leshner, M;Roloff, GW;True, LD;Misteli, T;Tissue-of-origin-specific gene repositioning in breast and prostate cancerHistochem. Cell Biol.26791532Tissue sections and tissue microarrays (TMAs) were purchased from US Biomax Inc, Imgenex Corporation, Folio Bioscience and Biochain Institute or were acquired from the University of Washington under the guidelines and approval of the Institutional ReFull Paper
2016Quadrini, KJ;Hegelund, AC;Cortes, KE;Xue, C;Kennelly, SM;Ji, H;Högerkorp, CM;Mc Closkey, TW;Validation of a flow cytometry-based assay to assess C5aR receptor occupancy on neutrophils and monocytes for use in drug developmentCytometry B Clin Cytom26084468a specific Disease Activity Score 28‐joint counts (DAS28) criteria of C‐reactive protein (CRP) ≥ 3.2 and ≥ 2 swollen joints 10, 11 was drawn into K 2 ‐EDTA phlebotomy tubes and stored at 2-8°C. RA patient samples were obtained from Conversant BiologiFull Paper
2016Gebauer, A;Schmidt, S;Hoffmann, W;Status and perspective of lab-on-a-chip systems for common diseases: a systematic review from 2003 to 2013Per Med29749869Diagnosis, monitoring Laboratory Patients with advanced-stage breast cancer 73/73 patients 5/5 negative control Conversant Bio (USA), bio options (USA) ? 61 Prospective experimental study Diagnosis, monitoring ? Patients with metastatic lung (n = 55)Full Paper
2016Shelton, D;Quinlan, A;Rapid, Sensitive Cancer Biomarker Screening in cfDNAGenetic Engineering & Biotechnology NewsUntested plasma samples from patients who had previously been classified as KRAS mutation positive or negative by FFPE genotyping were purchased from Conversant Bio and ProMedDx. Figure 3. Multiplex detection of KRAS mutations in patient cell-free DNFull Paper
2016Justice, JL;Weese, DA;Santos, SR;Phylogenetic utility, and variability in structure and content, of complete mitochondrial genomes among genetic lineages of the Hawaiian anchialine shrimp Halocaridina rubra Holthuis 1963 (Atyidae:Decapoda)Mitochondrial DNA A DNA Mapp Seq Anal26061341The Atyidae are caridean shrimp possessing hair-like setae on their claws and are important contributors to ecological services in tropical and temperate fresh and brackish water ecosystems. Complete mitochondrial genomes have only been reported fromFull Paper
2016Li, S;Garrett-Bakelman, FE;Chung, SS;Sanders, MA;Hricik, T;Rapaport, F;Patel, J;Dillon, R;Vijay, P;Brown, AL;Perl, AE;Cannon, J;Bullinger, L;Luger, S;Becker, M;Lewis, ID;To, LB;Delwel, R;Löwenberg, B;Döhner, H;Döhner, K;Guzman, ML;Hassane, DC;Roboz, GJ;Grimwade, D;Valk, PJ;D'Andrea, RJ;Carroll, M;Park, CY;Neuberg, D;Levine, R;Melnick, AM;Mason, CE;Distinct evolution and dynamics of epigenetic and genetic heterogeneity in acute myeloid leukemiaNature medicine27322744rough AML_101, DNA and RNA were extracted from mononuclear cell layers using standard techniques. Normal bone marrow controls (NBM: CD34+ mononuclear cells; 7 males and 7 females) were purchased from AllCells (Alameda, CA, USA; n = 5) or isolated usiFull Paper
2016Dever, DP;Bak, RO;Reinisch, A;Camarena, J;Washington, G;Nicolas, CE;Pavel-Dinu, M;Saxena, N;Wilkens, AB;Mantri, S;Uchida, N;Hendel, A;Narla, A;Majeti, R;Weinberg, KI;Porteus, MH;CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cellsNature27820943blood were purchased from AllCells and thawed according to manufacturer's instructions. CD34 + HSPCs from cord blood were either purchased frozen from AllCells or acquired from SaveFull Paper
2016Briney, B;Sok, D;Jardine, JG;Kulp, DW;Skog, P;Menis, S;Jacak, R;Kalyuzhniy, O;de Val, N;Sesterhenn, F;Le, KM;Ramos, A;Jones, M;Saye-Francisco, KL;Blane, TR;Spencer, S;Georgeson, E;Hu, X;Ozorowski, G;Adachi, Y;Kubitz, M;Sarkar, A;Wilson, IA;Ward, AB;Nemazee, D;Burton, DR;Schief, WR;Tailored Immunogens Direct Affinity Maturation toward HIV Neutralizing AntibodiesCell27610570pathogen free environment. Healthy, HIV-Negative Human PBMCs Leukopaks from two heathy, HIV-negative individuals (a 28 year-old female and a 33 year-old male) were obtained from a commercial vendor (AllCells) using a protocol approved by the InstituFull Paper
2016Corces, MR;Buenrostro, JD;Wu, B;Greenside, PG;Chan, SM;Koenig, JL;Snyder, MP;Pritchard, JK;Kundaje, A;Greenleaf, WJ;Majeti, R;Chang, HY;Lineage-specific and single-cell chromatin accessibility charts human hematopoiesis and leukemia evolutionNature genetics27526324Normal donor human bone marrow and peripheral blood cells were obtained fresh from AllCells or the Stanford Blood Center. All normal blood cell populations were sorted fresh. SaveFull Paper
2016Kim, JH;Hu, Y;Yongqing, T;Kim, J;Hughes, VA;Le Nours, J;Marquez, EA;Purcell, AW;Wan, Q;Sugita, M;Rossjohn, J;Winau, F;CD1a on Langerhans cells controls inflammatory skin diseaseNature immunology27548435lysis by ELISA. K562 cells were routinely tested for mycoplasma contamination, and CD1a expression of transfectants was confirmed before use by flow cytometry. PBMCs were obtained from ConversantBio, ALLCELLS, and the Kraft Family Blood Donor CenterFull Paper
2016Locci, M;Wu, JE;Arumemi, F;Mikulski, Z;Dahlberg, C;Miller, AT;Crotty, S;Activin A programs the differentiation of human TFH cellsNature immunology27376469Leukapheresis sample from a healthy donor was obtained from AllCells, Inc., after a prescreening of multiple donors. Fresh whole blood samples from healthy donors were obtained SaveFull Paper
2016Wang, X;Haylock, D;Hu, CS;Kowalczyk, W;Jiang, T;Qiu, J;Mosoyan, G;He, W;Marshall, N;Mascarenhas, J;Tarasova, A;Brody, J;Winkler, D;Hoffman, R;A thrombopoietin receptor antagonist is capable of depleting myelofibrosis hematopoietic stem and progenitor cellsBlood27114459Flow Cytometer (Becton Dickinson, Franklin Lakes, NJ) were used in each experiment. BM CD34+ cells from normal individuals were purchased from AllCells (Alameda, CA). The JAK2, CALR, and MPL mutational status1,22-24 of each patient is shown in TableFull Paper
2016Zhang, Y;Quarmyne, M;Himburg, H;Yan, X;McBride, W;Jung, M;Chute, J;A Small Molecule Inhibitor of Protein Tyrosine Phosphatase-Sigma (PTPσ) Promotes Hematopoietic Stem Cell (HSC) RegenerationBloodOur laboratory previously discovered that pleiotrophin, a paracrine growth factor secreted by the BM perivascular and endothelial cells, promoted HSC self-renewal and regeneration SaveFull Paper
2016Iancu-Rubin, C;Fong, H;Mosoyan, G;Patel, A;Sabado, R;Marcia, M;Moshier, E;Ozbek, U;Keyzner, A;Bhardwaj, N;McNiece, I;Tong, J;Hoffman, R;Preclinical Development of a Cord Blood (CB)-Derived Hematopoietic Stem Cell (HSC) Product for Allogeneic Transplantation in Patients with Hematological MalignanciesBloodDivision of Hematology and Medical Oncology, The Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY++AllCells, LLC, Alameda, CA++Division of Hematology and Medical Oncology, The Tisch Cancer Institute, Icahn School of MedicFull Paper
2016Hoff, F;Hu, C;Qiu, Y;Yoo, S;Qutub, A;Horton, T;Kornblau, S;Recurrent Patterns of Protein Expression Signatures in Pediatric Acute Lymphoblastic Leukemia: Recognition and Therapeutic GuidanceBloodreverse-phase protein arrays (RPPA) methodology was performed on 73 samples from pediatric patients with ALL, 10 cryopreserved CD34 + normal bone marrow samples (AllCells) SaveFull Paper
2016Yoshioka, S;Miura, Y;Iwasa, M;Fujishiro, A;Sugino, N;Fujii, S;Nakagawa, Y;Sato, A;Yokota, A;Hirai, H;Ichinohe, T;Takaori, A;Maekawa, T;Late Adherent Subpopulation in Umbilical Cord Blood Has the Same Characteristics and Hematopoiesis-Supporting Capacity As Mesenchymal Stromal/Stem CellsBloodMesenchymal stromal/stem cells (MSCs) are a major source of cell for cell therapy. MSCs derived from bone marrow (BMMSCs) have been mostly used in clinical applications. BMMSCs can be easily isolated as a cell population that adheres to plastic cultuFull Paper
2016Merkel, P;Giclas, H;Gibula, A;Andersen, G;Knight, V;Lin, J;Castro, G;Green, J;Stassinopoulos, A;Reinvestigation of the T-Cell Inactivation Achieved for the Prevention of TA GV HD By 2500 Gy of Gamma Irradiation, a Comparison with Pathogen ReductionBloodBackground: Transfusion Associated Graft vs. Host Disease (TA GVHD) results in high morbidity and mortality, caused by contaminating T-cells present in transfused blood products. Gamma irradiation (GI) of platelet components (PC) with a dose of 2500Full Paper
2016Kanter, J;Walters, M;Hsieh, M;Krishnamurti, L;Kwiatkowski, J;Kamble, R;von Kalle, C;Kuypers, F;Cavazzana, M;Leboulch, P;Joseney-Antoine, M;Asmal, M;Thompson, A;Tisdale, J;Interim Results from a Phase 1/2 Clinical Study of Lentiglobin Gene Therapy for Severe Sickle Cell DiseaseBloodβ-globin gene transfer into hematopoietic stem cells (HSCs) has the potential to reduce or eliminate the symptoms and long-term complications of severe sickle cell disease (SCD). LentiGlobin Drug Product (DP) is a gene therapy product containing autoFull Paper
2016Thompson, A;Kwiatkowski, J;Rasko, J;Hongeng, S;Schiller, G;Anurathapan, U;Cavazzana, M;Ho, P;von Kalle, C;Kletzel, M;Leboulch, P;Vichinsky, E;Petrusich, A;Asmal, M;Walters, M;Lentiglobin Gene Therapy for Transfusion-Dependent β-Thalassemia: Update from the Northstar Hgb-204 Phase 1/2 Clinical StudyBloodBACKGROUND Allogeneic hematopoietic stem cell (HSC) transplant is potentially curative for patients with β-thalassemia major or, as more broadly defined, transfusion dependent β-thalassemia (TDT). However, HSC transplant is generally rFull Paper
2016Crews, LA;Balaian, L;Delos Santos, NP;Leu, HS;Court, AC;Lazzari, E;Sadarangani, A;Zipeto, MA;La Clair, JJ;Villa, R;Kulidjian, A;Storb, R;Morris, SR;Ball, ED;Burkart, MD;Jamieson, CHM;RNA Splicing Modulation Selectively Impairs Leukemia Stem Cell Maintenance in Secondary Human AMLCell stem cell27570067Age-related human hematopoietic stem cell (HSC) exhaustion and myeloid-lineage skewing promote oncogenic transformation of hematopoietic progenitor cells into therapy-resistant SaveFull Paper
2016Song, C;Zhang, Y;Li, C;Chen, G;Kang, X;Wang, Q;Enhanced Nanodrug Delivery to Solid Tumors Based on a Tumor Vasculature-Targeted StrategyAdvanced Functional MaterialsCell Culture: Human malignantglioma U87-MG cells were obtained from The Cell Bank of Type Culture Collection of Chinese Academy of Sciences and HUVECs were purchased from Allcells (China). U87-MG cells were cultured in low-glucose Dulbecco's modifiedFull Paper
2016Sherbenou, DW;Aftab, BT;Su, Y;Behrens, CR;Wiita, A;Logan, AC;Acosta-Alvear, D;Hann, BC;Walter, P;Shuman, MA;Wu, X;Atkinson, JP;Wolf, JL;Martin, TG;Liu, B;Antibody-drug conjugate targeting CD46 eliminates multiple myeloma cellsThe Journal of clinical investigation27841764bead columns (Stem Cell Technologies). Primary cells were cultured in RPMI1640 media with 100 U/ml penicillin, 100 μg/ml streptomycin, 10% FBS, and 2 ng/ml IL-6. BM and PB controls from normal donors were purchased from AllCellsFull Paper
2016Jiang, X;Hu, C;Arnovitz, S;Bugno, J;Yu, M;Zuo, Z;Chen, P;Huang, H;Ulrich, B;Gurbuxani, S;Weng, H;Strong, J;Wang, Y;Li, Y;Salat, J;Li, S;Elkahloun, AG;Yang, Y;Neilly, MB;Larson, RA;Le Beau, MM;Herold, T;Bohlander, SK;Liu, PP;Zhang, J;Li, Z;He, C;Jin, J;Hong, S;Chen, J;miR-22 has a potent anti-tumour role with therapeutic potential in acute myeloid leukaemiaNature communications27116251(Axis-Shield, Oslo, Norway) according to the manufacturer’s manual. Human normal hematopoietic control cell samples The MNC normal control samples were isolated from normal BM cells purchased from AllCells, LLC (Emeryville, CA) by use of NycoPrep 1.Full Paper
2016van Overbeek, M;Capurso, D;Carter, MM;Thompson, MS;Frias, E;Russ, C;Reece-Hoyes, JS;Nye, C;Gradia, S;Vidal, B;Zheng, J;Hoffman, GR;Fuller, CK;May, AP;DNA Repair Profiling Reveals Nonrandom Outcomes at Cas9-Mediated BreaksMolecular cell27499295Mobilized peripheral blood HSCs (AllCells # mPB018F) were plated at 1.5 × 105/ml in DMEM/F12 Glutamax supplemented with 100× MNEAA solution, 1,000× 2-mercaptoethanol, 100× StemSpan CC100, 1,000× TPO, and 10,000× GM-CSFFull Paper
2016Wang, J;DeClercq, JJ;Hayward, SB;Li, PW;Shivak, DA;Gregory, PD;Lee, G;Holmes, MC;Highly efficient homology-driven genome editing in human T cells by combining zinc-finger nuclease mRNA and AAV6 donor deliveryNucleic acids research26527725Fresh purified CD8 + and CD4 + T cells were purchased from AllCells.com and maintained in X-VIVO 15 (Lonza, Basel, Switzerland) supplemented with 10% FBS, 2 mM l-glutamine, 1 SaveFull Paper
2016Beard, RS;Yang, X;Meegan, JE;Overstreet, JW;Yang, CG;Elliott, JA;Reynolds, JJ;Cha, BJ;Pivetti, CD;Mitchell, DA;Wu, MH;Deschenes, RJ;Yuan, SY;Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndromeNature communications27653213Full Paper
2016Zhao, Y;Wang, A;Zou, Y;Su, N;Loscalzo, J;Yang, Y;In vivo monitoring of cellular energy metabolism using SoNar, a highly responsive sensor for NAD(+)/NADH redox stateNature protocols27362337Cells of interest: we have used the following: NCI-H1299 non-small-cell lung cancer (ATCC, cat. no. CRL-5803), primary mouse hepatocytes (freshly isolated), primary mouse renal tubular epithelial cells (AllCells, cat. no. MRTEC-001F), primary mouse aFull Paper
2016Li, L;Qi, X;Sun, W;Abdel-Azim, H;Lou, S;Zhu, H;Prasadarao, NV;Zhou, A;Shimada, H;Shudo, K;Kim, YM;Khazal, S;He, Q;Warburton, D;Wu, L;Am80-GCSF synergizes myeloid expansion and differentiation to generate functional neutrophils that reduce neutropenia-associated infection and mortalityEMBO molecular medicine27737899opoietin. Primary cells were split every 3 days with equal portions of fresh MM‐HSCCM. Normal primary human hematopoietic precursors and cell culture Human umbilical cord blood CD34+ precursors (AllCells, Emeryville, CA) were expanded, as describedFull Paper
2016Sun, H;Lin, DC;Cao, Q;Guo, X;Marijon, H;Zhao, Z;Gery, S;Xu, L;Yang, H;Pang, B;Lee, VK;Lim, HJ;Doan, N;Said, JW;Chu, P;Mayakonda, A;Thomas, T;Forscher, C;Baloglu, E;Shacham, S;Rajalingam, R;Koeffler, HP;CRM1 Inhibition Promotes Cytotoxicity in Ewing Sarcoma Cells by Repressing EWS-FLI1-Dependent IGF-1 SignalingCancer research26956669IGFBP3 siRNA lentiviral plasmid (i010368) and EWS-FLI siRNAs (ABM Inc.); Matrigel anti-rabbit IgG and anti-mouse conjugated HRP antibodies (BD Biosciences). siRNA pools targeting DDIT3 and p53 were purchased from Dharmacon. Bone marrow cells from norFull Paper
2016Prabhala, RH;Fulciniti, M;Pelluru, D;Rashid, N;Nigroiu, A;Nanjappa, P;Pai, C;Lee, S;Prabhala, NS;Bandi, RL;Smith, R;Lazo-Kallanian, SB;Valet, S;Raje, N;Gold, JS;Richardson, PG;Daley, JF;Anderson, KC;Ettenberg, SA;Di Padova, F;Munshi, NC;Targeting IL-17A in multiple myeloma: a potential novel therapeutic approach in myelomaLeukemia26293646med consent in accordance with the Declaration of Helsinki and approved by the institutional review board (IRB) from Dana-Farber Cancer Institute. Healthy donor bone marrow samples were obtained from AllCells (Emeryville, CA). Myeloma cell-proliferaFull Paper
2016Su, LF;Del Alcazar, D;Stelekati, E;Wherry, EJ;Davis, MM;Antigen exposure shapes the ratio between antigen-specific Tregs and conventional T cells in human peripheral bloodProceedings of the National Academy of Sciences of the United States of America27681619Human HLA-DR4 umbilical cord blood was purchased from AllCells and from the New York Human HLA-DR4 umbilical cord bloods were purchased from AllCells and from the New SaveFull Paper
2016Ye, L;Wang, J;Tan, Y;Beyer, AI;Xie, F;Muench, MO;Kan, YW;Genome editing using CRISPR-Cas9 to create the HPFH genotype in HSPCs: An approach for treating sickle cell disease and β-thalassemiaProceedings of the National Academy of Sciences of the United States of America27601644according to the manufacturer’s instruction. Peripheral blood mobilized CD34+ HSPCs and bone marrow CD34+ HSPCs were purchased from AllCells and cultured in StemSpan SFEM (Stemcell Technologies) supplemented with human cytokines, 100 ng/mL SCF, 100 nFull Paper
2016Tarafder, S;Koch, A;Jun, Y;Chou, C;Awadallah, MR;Lee, CH;Micro-precise spatiotemporal delivery system embedded in 3D printing for complex tissue regenerationBiofabrication27108484MSCs were isolated from human bone marrow (AllCells, Alameda, CA) following our established protocol [5, 13]. P3-5 MSCs at a density of 1 × 10 6 cells ml −1 were seeded into the SaveFull Paper
2016Horiguchi, H;Kobune, M;Kikuchi, S;Yoshida, M;Murata, M;Murase, K;Iyama, S;Takada, K;Sato, T;Ono, K;Hashimoto, A;Tatekoshi, A;Kamihara, Y;Kawano, Y;Miyanishi, K;Sawada, N;Kato, J;Extracellular vesicle miR-7977 is involved in hematopoietic dysfunction of mesenchymal stromal cells via poly(rC) binding protein 1 reduction in myeloid neoplasmsHaematologica26802051MSCs from healthy volunteers (HVs) were purchased from AllCells, LLC (Toronto, Canada) (HV-derived MSCs #1, #2, #3 and #4). Human primary MSCs and human BM CD34+ cells derived from lymphoma patients stage I/II without bone marrow localization (controFull Paper
2016Li, D;Xie, K;Zhang, L;Yao, X;Li, H;Xu, Q;Wang, X;Jiang, J;Fang, J;Dual blockade of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF-2) exhibits potent anti-angiogenic effectsCancer letters27130666Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China) and maintained in a complete HUVEC medium with 10% FBS (Invitrogen) and 10% SaveFull Paper
2016de Jong, RN;Beurskens, FJ;Verploegen, S;Strumane, K;van Kampen, MD;Voorhorst, M;Horstman, W;Engelberts, PJ;Oostindie, SC;Wang, G;Heck, AJ;Schuurman, J;Parren, PW;A Novel Platform for the Potentiation of Therapeutic Antibodies Based on Antigen-Dependent Formation of IgG Hexamers at the Cell SurfacePLoS biology26736041nuously cultured systems to ensure authenticity of the cell lines. Pooled normal human serum (NHS) AB was obtained from Sanquin (The Netherlands). Primary B cells from CLL patients were obtained from AllCells (Alameda, CA). Animals Eight- to 11-wk-oFull Paper
2016Carmi, Y;Prestwood, TR;Spitzer, MH;Linde, IL;Chabon, J;Reticker-Flynn, NE;Bhattacharya, N;Zhang, H;Zhang, X;Basto, PA;Burt, BM;Alonso, MN;Engleman, EG;Akt and SHP-1 are DC-intrinsic checkpoints for tumor immunityJCI insight27812544For some experiments, BM cells and peripheral blood from the same donor were purchased from AllCells. The cells were applied to a Ficoll gradient (BD), and CD34 + cells were SaveFull Paper
2016Legemate, K;Tarafder, S;Jun, Y;Lee, CH;Engineering Human TMJ Discs with Protein-Releasing 3D-Printed ScaffoldsJournal of dental research27053116Human MSCs were isolated from commercially available, fresh whole bone marrow samples of anonymous adult donors (AllCells) (age range: 20-25 y) per our previous methods (Lee et al. 2009; Lee, Shah, et al. 2010; Lee et al. 2014). Following our establiFull Paper
2016Kota, DJ;Prabhakara, KS;van Brummen, AJ;Bedi, S;Xue, H;DiCarlo, B;Cox, CS;Olson, SD;Propranolol and Mesenchymal Stromal Cells Combine to Treat Traumatic Brain InjuryStem cells translational medicine26586775We isolated MSCs from commercially available fresh human bone marrow aspirates of a 34-year-old male (AllCells, Emeryville, CA, http://www.allcells.com) according to the protocols previously established using density centrifugation and plastic adhereFull Paper
2016Lee, SJ;Levitsky, K;Parlati, F;Bennett, MK;Arastu-Kapur, S;Kellerman, L;Woo, TF;Wong, AF;Papadopoulos, KP;Niesvizky, R;Badros, AZ;Vij, R;Jagannath, S;Siegel, D;Wang, M;Ahmann, GJ;Kirk, CJ;Clinical activity of carfilzomib correlates with inhibition of multiple proteasome subunits: application of a novel pharmacodynamic assayBritish journal of haematology27071340Human whole blood and peripheral blood mononuclear cell (PBMC)-enriched leukapheresis-derived samples from healthy volunteers were purchased from AllCells (Berkeley, CA, SaveFull Paper
2016Jones, RB;Mueller, S;O'Connor, R;Rimpel, K;Sloan, DD;Karel, D;Wong, HC;Jeng, EK;Thomas, AS;Whitney, JB;Lim, SY;Kovacs, C;Benko, E;Karandish, S;Huang, SH;Buzon, MJ;Lichterfeld, M;Irrinki, A;Murry, JP;Tsai, A;Yu, H;Geleziunas, R;Trocha, A;Ostrowski, MA;Irvine, DJ;Walker, BD;A Subset of Latency-Reversing Agents Expose HIV-Infected Resting CD4+ T-Cells to Recognition by Cytotoxic T-LymphocytesPLoS pathogens27082643the HIV-1 Test, v2.0 kit (Roche Diagnostics). Primary CD4+ T cell HIV latency model Total peripheral blood mononuclear cells (PBMCs) were obtained from healthy HIV-negative donors by leukapheresis (AllCells, Inc, Emeryville, CA). Naive CD4+ T cellsFull Paper
2016Clark, RS;Pellom, ST;Booker, B;Ramesh, A;Zhang, T;Shanker, A;Maguire, M;Juarez, PD;Patricia, MJ;Langston, MA;Lichtveld, MY;Hood, DB;Validation of research trajectory 1 of an Exposome framework: Exposure to benzo(a)pyrene confers enhanced susceptibility to bacterial infectionEnvironmental research26765097Fresh human CD14+ monocytes were obtained from AllCells (Alameda, CA) and were cultured in RPMI 1640 medium supplemented with 10% heat-inactivated FBS (HyClone), 100 U/penicillin, 100 U/mL streptomycin (GIBCO) in the presence of DMSO, B(a)P (1, 5, 10Full Paper
2016Zhang, W;Cao, H;Zhang, X;Li, G;Chang, Q;Zhao, J;Qiao, Y;Ding, X;Yang, G;Liu, X;Jiang, X;A strontium-incorporated nanoporous titanium implant surface for rapid osseointegrationNanoscale26881868In this experiment, endothelial cell growth medium (AllCells) was used to collect the secreted growth factors, and then was taken for HUVECS culture for 24 hours to form cellular SaveFull Paper
2016Wu, J;Sun, Y;Block, TJ;Marinkovic, M;Zhang, ZL;Chen, R;Yin, Y;Song, J;Dean, DD;Lu, Z;Chen, XD;Umbilical cord blood-derived non-hematopoietic stem cells retrieved and expanded on bone marrow-derived extracellular matrix display pluripotent characteristicsStem cell research & therapy27906056Freshly isolated human BM-MNCs (containing MSCs), obtained from 20- to 30-year-old donors and purchased from ALLCELLS (Emeryville, CA, USA), were used to prepare BM stromal SaveFull Paper
2016Umemura, N;Ohkoshi, E;Tajima, M;Kikuchi, H;Katayama, T;Sakagami, H;Hyaluronan induces odontoblastic differentiation of dental pulp stem cells via CD44Stem cell research & therapy27651223s modified Eagle’s medium (DMEM) was purchased from Invitrogen (Carlsbad, CA, USA). Fetal bovine serum (FBS) was purchased from Nichirei Bioscience (Tokyo, Japan). Human DPSCs were obtained from AllCells LLC (Emeryville, CA, USA). Cell cultures wereFull Paper
2016Frasheri, I;Ern, C;Diegritz, C;Hickel, R;Hristov, M;Folwaczny, M;Full-length amelogenin influences the differentiation of human dental pulp stem cellsStem cell research & therapy26762641The human dental pulp stem cells hDPSCs were obtained from AllCells (Alameda, CA, USA). They had been characterized by the company by means of flow cytometry, revealing a SaveFull Paper
2016Chen, W;Wang, S;Adhikari, S;Deng, Z;Wang, L;Chen, L;Ke, M;Yang, P;Tian, R;Simple and Integrated Spintip-Based Technology Applied for Deep Proteome ProfilingAnalytical chemistry27062885The SHED cell line was purchased from Allcells (Alameda, CA) and cultured in α-modified Eagle's medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/ SaveFull Paper
2016Gonçalves Silva, I;Rüegg, L;Gibbs, BF;Bardelli, M;Fruehwirth, A;Varani, L;Berger, SM;Fasler-Kan, E;Sumbayev, VV;The immune receptor Tim-3 acts as a trafficker in a Tim-3/galectin-9 autocrine loop in human myeloid leukemia cellsOncoimmunology27622049). All other chemicals purchased were of the highest grade of purity. Primary human AML and CLL cells Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordaFull Paper
2016Yang, J;Liu, W;Lv, J;Feng, Y;Ren, X;Zhang, W;REDV-polyethyleneimine complexes for selectively enhancing gene delivery in endothelial cellsJournal of materials chemistry. B32263272The pEGFP-ZNF580 plasmid (pZNF580) was preserved by Department of Physiology and Pathophysiology, Logistics University of Chinese People's Armed Police Force. CREDVW and CRE[V with combining low line][D with combining low line]W peptides were supplieFull Paper
2016Ma, SD;Xu, X;Jones, R;Delecluse, HJ;Zumwalde, NA;Sharma, A;Gumperz, JE;Kenney, SC;PD-1/CTLA-4 Blockade Inhibits Epstein-Barr Virus-Induced Lymphoma Growth in a Cord Blood Humanized-Mouse ModelPLoS pathogens27186886diabetic/severe combined immunodeficient (NOD/LtSz-scid/IL2Rγnull) mice were purchased from Jackson Labs (catalogue 005557). Commercially purchased CD34-depleted human cord blood mononuclear cells (AllCells, LLC., CB117) were mock-infected, or infectFull Paper
2016Ebina, W;Combinatorial Pathway Modulation Toward Ex Vivo Maintenance and Propagation of Hematopoietic Stem CellsThesisCD34 enriched human cord blood, mobilized peripheral blood, or human bone marrow (AllCells) were thawed, washed in PBS with 2mM EDTA and 2%FBS, and stained for the following markers: Lineage(CD235a, CD3, CD19, CD20, CD56), CD34, CD38, CD45RA, and CD9Full Paper
2016Yang, T;Yao, Q;Cao, F;Liu, Q;Liu, B;Wang, XH;Silver nanoparticles inhibit the function of hypoxia-inducible factor-1 and target genes: insight into the cytotoxicity and antiangiogenesisInternational journal of nanomedicine27994464nd the human lymphoma cell lines Raji and Daudi were obtained from the American Type Culture Collection (Manassas, VA, USA). Human umbilical vein endothelial cells (HUVEC) were obtained from Shanghai AllCells Biotech Co. Ltd. (Shanghai, People’s RepuFull Paper
2016Moot, R;Raikar, SS;Fleischer, L;Querrey, M;Tylawsky, DE;Nakahara, H;Doering, CB;Spencer, HT;Genetic engineering of chimeric antigen receptors using lamprey derived variable lymphocyte receptorsMolecular therapy oncolytics27933313d using a BD FACS Aria II Cell Sorter (BD Biosciences). Expansion and lentiviral transduction of primary human T cells Commercially available human peripheral blood mononuclear cells were purchased (AllCells, Catalog # PB004F, Alameda, CA). After thFull Paper
2016Hall, BM;Balan, V;Gleiberman, AS;Strom, E;Krasnov, P;Virtuoso, LP;Rydkina, E;Vujcic, S;Balan, K;Gitlin, I;Leonova, K;Polinsky, A;Chernova, OB;Gudkov, AV;Aging of mice is associated with p16(Ink4a)- and β-galactosidase-positive macrophage accumulation that can be induced in young mice by senescent cellsAging27391570f p16(Ink4a)-positive cells [21,23] and may reflect an exaggerated view on SCs as the sole source of inflammaging. MATERIALS AND METHODS Cell culture Primary human neonatal dermal fibroblasts (NDFs; AllCells, LLC) were pooled equally from three sepaFull Paper
2016Naka, K;Ishihara, K;Jomen, Y;Jin, CH;Kim, DH;Gu, YK;Jeong, ES;Li, S;Krause, DS;Kim, DW;Bae, E;Takihara, Y;Hirao, A;Oshima, H;Oshima, M;Ooshima, A;Sheen, YY;Kim, SJ;Kim, DK;Novel oral transforming growth factor-β signaling inhibitor EW-7197 eradicates CML-initiating cellsCancer science26583567Full Paper
2016Rovira Gonzalez, YI;Lynch, PJ;Thompson, EE;Stultz, BG;Hursh, DA;In vitro cytokine licensing induces persistent permissive chromatin at the Indoleamine 2,3-dioxygenase promoterCytotherapy27421739Bone marrow-derived MSCs were obtained from AllCells (Alameda, CA), Lonza (Allendale, NJ), and RoosterBio (Frederick, MD) at passage one (Supplemental Table 1). MSCs in this study were previously expanded, characterized, and cryopreserved to generateFull Paper
2016Sutton, KS;Dasgupta, A;McCarty, D;Doering, CB;Spencer, HT;Bioengineering and serum free expansion of blood-derived γδ T cellsCytotherapy27260209Commercially available PBMCs were obtained via Leuko Pak apheresis from 11 healthy donors (AllCells). Nine frozen and two fresh PBMCs were used. Frozen PBMCs were rapidly SaveFull Paper
2016Stultz, BG;McGinnis, K;Thompson, EE;Lo Surdo, JL;Bauer, SR;Hursh, DA;Chromosomal stability of mesenchymal stromal cells during in vitro cultureCytotherapy26780865Both AllCells and Lonza use standard protocols for isolation of MSCs from bone marrow and culture cells in Mesencult (Stem Cell Technologies) or MSCGM (Lonza), respectively. SaveFull Paper
2016Imamichi, Y;Yuhki, KI;Orisaka, M;Kitano, T;Mukai, K;Ushikubi, F;Taniguchi, T;Umezawa, A;Miyamoto, K;Yazawa, T;11-Ketotestosterone Is a Major Androgen Produced in Human GonadsThe Journal of clinical endocrinology and metabolism27428878Plasma was separated by centrifugation at 1000g for 5 minutes. Other plasma samples were purchased from AllCells and ProMedDX. All plasma samples were collected under IRB-approved collection protocols and subject informed consent. The donors were 10Full Paper
2016Tsiang, M;Jones, GS;Goldsmith, J;Mulato, A;Hansen, D;Kan, E;Tsai, L;Bam, RA;Stepan, G;Stray, KM;Niedziela-Majka, A;Yant, SR;Yu, H;Kukolj, G;Cihlar, T;Lazerwith, SE;White, KL;Jin, H;Antiviral Activity of Bictegravir (GS-9883), a Novel Potent HIV-1 Integrase Strand Transfer Inhibitor with an Improved Resistance ProfileAntimicrobial agents and chemotherapy27645238the pseudotyped particles from SODk1 2G cells has been previously described (29). Freshly isolated human peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers (supplied by AllCells, Inc., Alameda, CA). Blood donors were neFull Paper
2016Feng, JY;Xu, Y;Barauskas, O;Perry, JK;Ahmadyar, S;Stepan, G;Yu, H;Babusis, D;Park, Y;McCutcheon, K;Perron, M;Schultz, BE;Sakowicz, R;Ray, AS;Role of Mitochondrial RNA Polymerase in the Toxicity of Nucleotide Inhibitors of Hepatitis C VirusAntimicrobial agents and chemotherapy26596942Normal human primary bone marrow (BM) light-density cells were from three different lots obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD). Primary rat neonatal SaveFull Paper
2016Yoon, J;Wang, H;Kim, YC;Yoshimoto, M;Abbasi, S;Morse Iii, HC;Plasma cell alloantigen ENPP1 is expressed by a subset of human B cells with potential regulatory functionsImmunology and cell biology270298961 mm sodium pyruvate and HEPES) immediately before each experiment. Cord blood cells were purchased from AllCells LLC (Emeryville, CA. USA). Tonsillar tissues were kindly provided by Dr Leonid B Margolis (National Institute of Child Health and HumanFull Paper
2016Marklein, RA;Lo Surdo, JL;Bellayr, IH;Godil, SA;Puri, RK;Bauer, SR;High Content Imaging of Early Morphological Signatures Predicts Long Term Mineralization Capacity of Human Mesenchymal Stem Cells upon Osteogenic InductionStem cells (Dayton, Ohio)26865267Human bone marrow-derived stem cells were obtained from eight different donors purchased from either Lonza (Walkersville, MD, www.lonza.com) (167696, 110877, 8F3560, 127756) or All Cells (Emeryville, CA, www.allcells.com) (PCBM1632, PCBM1641, PCBM165Full Paper
2016Rosa, V;Xie, H;Dubey, N;Madanagopal, TT;Rajan, SS;Morin, JL;Islam, I;Castro Neto, AH;Graphene oxide-based substrate: physical and surface characterization, cytocompatibility and differentiation potential of dental pulp stem cellsDental materials : official publication of the Academy of Dental Materials27283997The use of human cells in this study was approved by NUS Institutional Review Board (Approval Number: NUS 2094). Human dental pulp stem cells from single donor (DPF003, Allcells, USA) were sorted by fluorescence-activated cell sorting analysis (FACS,Full Paper
2016Wang, J;Zhang, Y;Zhu, Q;Liu, Y;Cheng, H;Zhang, Y;Li, T;Emodin protects mice against radiation-induced mortality and intestinal injury via inhibition of apoptosis and modulation of p53Environmental toxicology and pharmacology27525562The aim of this study was to explore the protective effect of emodin, a plant-derived anthraquinone, against gamma radiation-induced mortality and intestinal injury in mice, and to SaveFull Paper
2016Walters, MC;De Castro, LM;Sullivan, KM;Krishnamurti, L;Kamani, N;Bredeson, C;Neuberg, D;Hassell, KL;Farnia, S;Campbell, A;Petersdorf, E;Indications and Results of HLA-Identical Sibling Hematopoietic Cell Transplantation for Sickle Cell DiseaseBiology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation26500093Financial disclosure: MCW is medical director for the ViaCord Processing Laboratory and AllCells, Inc. He is a consultant for bluebird bio, Inc, Sangamo Biosciences, Inc and Bayer. SaveFull Paper
2016Hsu, H;Boudova, S;Mvula, G;Divala, TH;Mungwira, RG;Harman, C;Laufer, MK;Pauza, CD;Cairo, C;Prolonged PD1 Expression on Neonatal Vδ2 Lymphocytes Dampens Proinflammatory Responses: Role of Epigenetic RegulationJournal of immunology (Baltimore, Md. : 1950)27474072commercial specimens obtained from AllCells. Although we cannot exclude that we introduced a bias in our study, this seems unlikely considering that the AllCells specimens were not SaveFull Paper
2016Chen, LT;Hu, MM;Xu, ZS;Liu, Y;Shu, HB;MSX1 Modulates RLR-Mediated Innate Antiviral Signaling by Facilitating Assembly of TBK1-Associated ComplexesJournal of immunology (Baltimore, Md. : 1950)27194789Generation and transfection of human primary macrophages were performed, as previously described (36). The human CD14+ monocytes were purchased from ALLCELLS. For generation of macrophages, the CD14+ monocytes were treated with recombinant M-CSF (50Full Paper
2016Gao, W;Liu, H;Yuan, J;Wu, C;Huang, D;Ma, Y;Zhu, J;Ma, L;Guo, J;Shi, H;Zou, Y;Ge, J;Exosomes derived from mature dendritic cells increase endothelial inflammation and atherosclerosis via membrane TNF-α mediated NF-κB pathwayJournal of cellular and molecular medicine27515767P (Ribobio, Guangzhou, China) was used. SiRNA TNF‐α was also purchased from Ribobio and the transfected concentration was 50 nM. HUVECs culture and intervention Primary HUVECs were purchased from AllCells (Shanghai, China) and cultured with a speciaFull Paper
2016Zhou, Y;Li, XH;Zhang, CC;Wang, MJ;Xue, WL;Wu, DD;Ma, FF;Li, WW;Tao, BB;Zhu, YC;Hydrogen sulfide promotes angiogenesis by downregulating miR-640 via the VEGFR2/mTOR pathwayAmerican journal of physiology. Cell physiology26879375Pooled human umbilical vein endothelial cells (HUVECs) were purchased from Allcells (Shanghai, China) and cultured in endothelial basal medium (EBM; Allcells) supplemented with SaveFull Paper
2016Lowery, JW;Ankrum, JA;Kokabu, S;Liu, R;Targeted Strategies to Modulate Stem-Cell-Relevant PathwaysStem cells international27703484+ CB progenitors (also referred to as "starting CB progenitors") from pooled donors were purchased from AllCells (Emeryville, CA) and cultured in the hematopoietic growth medium ( SaveFull Paper
2016Nagaria, PK;Robert, C;Park, TS;Huo, JS;Zambidis, ET;Rassool, FV;High-Fidelity Reprogrammed Human IPSCs Have a High Efficacy of DNA Repair and Resemble hESCs in Their MYC Transcriptional SignatureStem cells international27688775+ CB progenitors (also referred to as "starting CB progenitors") from pooled donors were purchased from AllCells (Emeryville, CA) and cultured in the hematopoietic growth medium ( SaveFull Paper
2016Shibata, T;Ohto, U;Nomura, S;Kibata, K;Motoi, Y;Zhang, Y;Murakami, Y;Fukui, R;Ishimoto, T;Sano, S;Ito, T;Shimizu, T;Miyake, K;Guanosine and its modified derivatives are endogenous ligands for TLR7International immunology26489884Human macrophages were induced from human peripheral blood mononuclear cells (hPBMCs), purchased from Allcells (Alameda, CA, USA) and Cellular Technology Limited (Shaker Height, OH, USA). hPBMCs were plated in 10-cm cell-culture dishes with a densityFull Paper
2016Pineda, G;Lennon, KM;Delos Santos, NP;Lambert-Fliszar, F;Riso, GL;Lazzari, E;Marra, MA;Morris, S;Sakaue-Sawano, A;Miyawaki, A;Jamieson, CH;Tracking of Normal and Malignant Progenitor Cell Cycle Transit in a Defined NicheScientific reports27041210amples were obtained from patient peripheral blood that was collected with informed consent for research use in accordance with Institutional Review Board approved protocols at UCSD or purchased from AllCells (Alameda, CA). Normal peripheral blood moFull Paper
2016Zou, L;Chen, Q;Quanbeck, Z;Bechtold, JE;Kaufman, DS;Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cellsScientific reports26980556Human BM-MSCs were isolated from fresh bone marrow (AllCells) according to the recommended protocol and expanded in MSC culture media, consisting of 10% defined fetal bovine SaveFull Paper
2016Ling, C;Bhukhai, K;Yin, Z;Tan, M;Yoder, MC;Leboulch, P;Payen, E;Srivastava, A;High-Efficiency Transduction of Primary Human Hematopoietic Stem/Progenitor Cells by AAV6 Vectors: Strategies for Overcoming Donor-Variation and Implications in Genome EditingScientific reports27759036ary HSPCs derived from bone marrow (BM) as well as from umbilical cord blood (CB). BM-derived CD34+ cells from individual donors (or a mixture from 10 donors) were purchased from a commercial source (AllCells, LLC, Alameda, CA, USA), and were used toFull Paper
2016Tian, GA;Zhu, CC;Zhang, XX;Zhu, L;Yang, XM;Jiang, SH;Li, RK;Tu, L;Wang, Y;Zhuang, C;He, P;Li, Q;Cao, XY;Cao, H;Zhang, ZG;CCBE1 promotes GIST development through enhancing angiogenesis and mediating resistance to imatinibScientific reports27506146HUVECs were obtained from the Fifth People's Hospital of Shanghai and cultured in endothelial cell complete medium containing endothelial cell growth supplement (Allcells; H-004). SaveFull Paper
2016Kim, YC;Cui, J;Luo, J;Xiao, F;Downs, B;Wang, SM;Exome-based Variant Detection in Core PromotersScientific reports27464681Caucasian male donor were purchased from AllCells Company (AllCells, Alameda, California). The use of the cells for research purpose has AllCells company IRB approval (Biomed SaveFull Paper
2016Chalmers, SA;Doerner, J;Bosanac, T;Khalil, S;Smith, D;Harcken, C;Dimock, J;Der, E;Herlitz, L;Webb, D;Seccareccia, E;Feng, D;Fine, JS;Ramanujam, M;Klein, E;Putterman, C;Therapeutic Blockade of Immune Complex-Mediated Glomerulonephritis by Highly Selective Inhibition of Bruton's Tyrosine KinaseScientific reports27192942Primary CD19+ cells were purified from human peripheral blood mononuclear cells (AllCells frozen peripheral blood mononuclear cells (AllCells) and negatively selected by magnetic SaveFull Paper
2016Moisseiev, E;Smit-McBride, Z;Oltjen, S;Zhang, P;Zawadzki, RJ;Motta, M;Murphy, CJ;Cary, W;Annett, G;Nolta, JA;Park, SS;Intravitreal Administration of Human Bone Marrow CD34+ Stem Cells in a Murine Model of Retinal DegenerationInvestigative ophthalmology & visual science27537262Fresh human BM aspirate was purchased from AllCells (Alameda, CA, USA) and obtained for the scheduled day of use. The BM aspirate for this study was obtained from a 38-year-old SaveFull Paper
2016Hassan, HE;Keita, JA;Narayan, L;Brady, SM;Frederick, R;Carlson, S;C Glass, K;Natesan, S;Buttolph, T;Fandy, TE;The combination of dimethoxycurcumin with DNA methylation inhibitor enhances gene re-expression of promoter-methylated genes and antagonizes their cytotoxic effectEpigenetics27588609Frozen acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) bone marrow mononuclear cells (BMNC) were purchased from Allcells (Alameda, CA). BMNC were obtained from newly diagnosed and untreated patients. The viability of the cells was tesFull Paper
2016Hu, X;Hu, T;Shen, G;Lian, M;Guan, G;Wang, F;Wang, L;PCL films of varying porosity influence ICAM-1 expression of HUVECsJournal of biomedical materials research. Part A27345288The HUVECs (HUVEC001) provided by Allcells Company (Shanghai, China) were cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS, Gibco), and 1% penicillin-streptomycin (Gibco) at 37°C in a 5% CO2 incubator. Before seeFull Paper
2016Lin, HA;Gupta, MS;Varma, DM;Gilchrist, ML;Nicoll, SB;Lower crosslinking density enhances functional nucleus pulposus-like matrix elaboration by human mesenchymal stem cells in carboxymethylcellulose hydrogelsJournal of biomedical materials research. Part A26256108Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density gradient centrifugation as previously described.18 Briefly, bone marrow aspirate was SaveFull Paper
2016Rogers, LJ;Basnakian, AG;Orloff, MS;Ning, B;Yao-Borengasser, A;Raj, V;Kadlubar, S;2-amino-1-methyl-6-phenylimidazo(4,5-b) pyridine (PhIP) induces gene expression changes in JAK/STAT and MAPK pathways related to inflammation, diabetes and cancerNutrition & metabolism27547236med by submission to American Type Culture Collection (ATCC) (Manassas, VA) for analysis by short tandem repeat methodology on December 9, 2015. Human Mesenchymal Stem Cells (hMSC) were obtained from AllCells (Emeryville, CA). The hMSCs were expandedFull Paper
2016Pellegrino, M;Sciambi, A;Yates, JL;Mast, JD;Silver, C;Eastburn, DJ;RNA-Seq following PCR-based sorting reveals rare cell transcriptional signaturesBMC genomics27189161Anonymous blood samples were initially obtained from a commercial provider (AllCells) that operates with independent IRB approval. Donor anonymity was protected using United SaveFull Paper
2016Holt, A;Wootton, SC;Mulero, JJ;Brzoska, PM;Langit, E;Green, RL;Developmental validation of the Quantifiler(®) HP and Trio Kits for human DNA quantification in forensic samplesForensic science international. Genetics26774100Human female DNA "A3408" was purified from peripheral blood cells obtained from AllCells LLC. (Alameda, CA) using a QIAamp DNA Blood Maxi Kit (Qiagen). SaveFull Paper
2016Pankajakshan, D;Albuquerque, MT;Evans, JD;Kamocka, MM;Gregory, RL;Bottino, MC;Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell FunctionJournal of endodontics27663615After 7 days, the specimens were rinsed with 10 mL saline followed by 3 mL EDTA for 3 minutes, and a final abundant rinsing step with saline was performed before cell seeding. Human DPSCs (AllCells, LLC, Alameda, CA) were cultured in alpha-minimum esFull Paper
2016Nicoletti, AM;Kenny, CH;Khalil, AM;Pan, Q;Ralph, KL;Ritchie, J;Venkataramani, S;Presky, DH;DeWire, SM;Brodeur, SR;Unexpected Potency Differences between B-Cell-Activating Factor (BAFF) Antagonist Antibodies against Various Forms of BAFF: Trimer, 60-Mer, and Membrane-BoundThe Journal of pharmacology and experimental therapeutics27440419Negatively selected B cells were obtained from frozen human peripheral blood (AllCells, LLC, Alameda, CA). Sexes of cell donors were as follows: Fig. 2C = 60-mer, two males, one SaveFull Paper
2016Jin, E;Kim, TH;Han, S;Kim, SW;Amniotic epithelial cells promote wound healing in mice through high epithelialization and engraftmentJournal of tissue engineering and regenerative medicine26174407Human AMEs were from AllCells (Alameda, CA, USA) and adipose-derived mesenchymal stem cells (ADMs) were from Thermo Scientific Inc. (Rockford, IL, USA). Human keratinocytes were also purchased from ATCC. Cell culture was performed as described previoFull Paper
2016Yang, H;Sun, J;Li, Y;Duan, WM;Bi, J;Qu, T;Human umbilical cord-derived mesenchymal stem cells suppress proliferation of PHA-activated lymphocytes in vitro by inducing CD4(+)CD25(high)CD45RA(+) regulatory T cell production and modulating cytokine secretionCellular immunology26774852PBMCs of healthy donors (n = 10) were purchased from AllCells Inc (Emeryville, CA, USA) and StemExpress (Placerville, CA, USA). Co-culture experiments were performed in a 12-well plate (BD, Franklin Lakes, New Jersey, USA). 1 × 105 UC-MSCs were plateFull Paper
2016Foster, MH;Buckley, ES;Chen, BJ;Hwang, KK;Clark, AG;Uncommon structural motifs dominate the antigen binding site in human autoantibodies reactive with basement membrane collagenMolecular immunology27450516100 cGy, X-RAD 320, Precision X-ray, North Branford, CT) 9.5-10 week old NSG recipients were intravenously injected with purified human CD34+ HSC (AllCells LLC, Alameda, CA), using 2.5-10×104 CD34+ HSC per mouse (Pearson et al., 2008; Shultz et al.,Full Paper
2016Smith, AJ;Li, Y;Bazin, HG;St-Jean, JR;Larocque, D;Evans, JT;Baldridge, JR;Evaluation of novel synthetic TLR7/8 agonists as vaccine adjuvantsVaccine27402566Human myeloid or plasmacytoid DCs were isolated from PBMCs obtained by leukapheresis from normal donors (AllCells, Berkeley, CA). PBMCs were repeatedly washed in 1× PBS SaveFull Paper
2016Zeng, Y;Liu, J;Role of glypican-1 in endothelial NOS activation under various steady shear stress magnitudesExperimental cell research27688027Human umbilical vein endothelial cells (HUVECs) and all cell culture reagents were purchased from Allcells, China. As previously described [23], HUVECs were cultured in HUVEC medium containing 10% FBS and 10% growth factor supplement containing EGF,Full Paper
2016Liu, X;Zhang, A;Xiang, J;Lv, Y;Zhang, X;miR-451 acts as a suppressor of angiogenesis in hepatocellular carcinoma by targeting the IL-6R-STAT3 pathwayOncology reports27461244The umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Shanghai, China). HepG2 and HEK293T cells were incubated with Dulbecco's modified Eagle's medium ( SaveFull Paper
2016Peterson, SJ;Vanella, L;Gotlinger, K;Jiang, H;Singh, SP;Sodhi, K;Maher, E;O'Hanlon, K;Shapiro, JI;Abraham, NG;Oxidized HDL is a potent inducer of adipogenesis and causes activation of the Ang-II and 20-HETE systems in human obese femalesProstaglandins & other lipid mediators27179555Frozen human bone marrow mononuclear stem cells (MSCs) were purchased from Allcells (Allcells, Emeryville, CA). After thawing, mononuclear cells were resuspended in a minimal essential medium, (Invitrogen, Carlsbad, CA) supplemented with 10% heat inaFull Paper
2016Ren, X;Li, J;Xu, X;Wang, C;Cheng, Y;IBI302, a promising candidate for AMD treatment, targeting both the VEGF and complement system with high binding affinity in vitro and effective targeting of the ocular tissue in healthy rhesus monkeysExperimental eye research26919788HUVEC basal medium (used for HUVEC proliferation assay) was bought from Allcells (Shanghai, China). Rabbit red blood cells (RRBCs) were purchased from Laboratory animal SaveFull Paper
2016Griffin, DO;Goff, SP;Restriction of HIV-1-based lentiviral vectors in adult primary marrow-derived and peripheral mobilized human CD34+ hematopoietic stem and progenitor cells occurs prior to viral DNA integrationRetrovirology26945863ep toward uncovering the molecular mechanism restricting HIV-1 transduction of HPSCs. Methods Source of human cells We obtained primary adult marrow-derived and peripheral mobilized CD34+ cells from AllCells and StemCell Technologies (pre-isolated).Full Paper
2016Cao, L;Weetall, M;Bombard, J;Qi, H;Arasu, T;Lennox, W;Hedrick, J;Sheedy, J;Risher, N;Brooks, PC;Trifillis, P;Trotta, C;Moon, YC;Babiak, J;Almstead, NG;Colacino, JM;Davis, TW;Peltz, SW;Discovery of Novel Small Molecule Inhibitors of VEGF Expression in Tumor Cells Using a Cell-Based High Throughput Screening PlatformPloS one27992500All primary cells were purchased from AllCells, Inc (California, USA) and cultured in RPMI-1640 medium for peripheral blood mononuclear cells (PBMC) or DMEM for adherent cells, SaveFull Paper
2016Pogue, SL;Taura, T;Bi, M;Yun, Y;Sho, A;Mikesell, G;Behrens, C;Sokolovsky, M;Hallak, H;Rosenstock, M;Sanchez, E;Chen, H;Berenson, J;Doyle, A;Nock, S;Wilson, DS;Targeting Attenuated Interferon-α to Myeloma Cells with a CD38 Antibody Induces Potent Tumor Regression with Reduced Off-Target ActivityPloS one27611189Bone Marrow cells: Normal and myeloma bone marrow cells were purchased from AllCells, Inc. as frozen vials containing ten million bone marrow mononuclear cells/vial. All donors SaveFull Paper
2016Doss, JF;Jonassaint, JC;Garrett, ME;Ashley-Koch, AE;Telen, MJ;Chi, JT;Phase 1 Study of a Sulforaphane-Containing Broccoli Sprout Homogenate for Sickle Cell DiseasePloS one27071063preferably the same time of day, once daily for 21 days. Patients consumed one (50g), two (100g) or three (150g) packages, depending on the dose. Cell culture Mobilized peripheral blood CD34+ cells (AllCells) were grown in StemLine II media containiFull Paper
2016Maifeld, SV;Ro, B;Mok, H;Chu, M;Yu, L;Yamagata, R;Leonardson, T;Chio, V;Parhy, B;Park, S;Carlson, M;Machhi, S;Ulbrandt, N;Falsey, AR;Walsh, EE;Wang, CK;Esser, MT;Zuo, F;Development of Electrochemiluminescent Serology Assays to Measure the Humoral Response to Antigens of Respiratory Syncytial VirusPloS one27070145serum samples showing a ≥4-fold rise in at least one ELISA assay. Thirty pediatric serum samples were purchased from Bioreclamation (Hicksville, NY). An adult human plasma sample was purchased from AllCells (Emeryville, CA) and subsequently used as tFull Paper
2016Smith, SH;Peredo, CE;Takeda, Y;Bui, T;Neil, J;Rickard, D;Millerman, E;Therrien, JP;Nicodeme, E;Brusq, JM;Birault, V;Viviani, F;Hofland, H;Jetten, AM;Cote-Sierra, J;Development of a Topical Treatment for Psoriasis Targeting RORγ: From Bench to SkinPloS one26870941ions were carried out in triplicate. Sequences of primers for ChIP-qPCR are listed in S1 Appendix. Human peripheral blood CD4+ T cell cultures and cytokine analysis Cryopreserved human CD4+ T cells (AllCells, LLC and Stemcell Technologies, Inc.) werFull Paper
2016Toby, GG;Liu, T;Buyue, Y;Zhang, X;Bitonti, AJ;Pierce, GF;Sommer, JM;Jiang, H;Peters, RT;Recombinant Factor IX Fc Fusion Protein Maintains Full Procoagulant Properties and Exhibits Prolonged Efficacy in Hemophilia B MicePloS one26840952e preparation (L-α-phosphatidylserine [PS] and L-α-phosphatidylcholine [PC]) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Fresh platelets from normal volunteers were purchased from AllCells, LLC (Emeryville, CA, USA). The mini-extrudFull Paper
2016Rebbapragada, I;Birkus, G;Perry, J;Xing, W;Kwon, H;Pflanz, S;Molecular Determinants of GS-9620-Dependent TLR7 ActivationPloS one26784926Human PBMCs were isolated from fresh whole blood derived from healthy human volunteers (AllCells) using standard Ficoll density gradient separation techniques. PBMCs were SaveFull Paper
2016Matsushita, M;Otsuka, Y;Tsutsumida, N;Tanaka, C;Uchiumi, A;Ozawa, K;Suzuki, T;Ichikawa, D;Aburatani, H;Okamoto, S;Kawakami, Y;Hattori, Y;Identification of Novel HLA-A*24:02-Restricted Epitope Derived from a Homeobox Protein Expressed in Hematological MalignanciesPloS one26784514D, Oslo, Norway) and cryopreserved in Cell Banker reagent (Juji-Field, Tokyo, Japan) in liquid nitrogen until further use. Bone marrow mononuclear cells (BMMNC) from healthy donors were obtained from AllCells, LLC. (Alameda, CA). This study was approFull Paper
2016Manser, M;Influence of extremely-low-frequency magnetic fields on epigenetic programming and cellular differentiationThesistrichostatin A (TSA). CD34-positive cells isolated from human cord blood of mixed donors were obtained from AllCells (Alabama, United States). CD34+ cells were propagated in Stemline II medium expansion medium (Stemcell technology) supplemented withFull Paper
2016Cabrero, M;Wei, Y;Yang, H;Ganan-Gomez, I;Bohannan, Z;Colla, S;Marchesini, M;Bravo, GM;Takahashi, K;Bueso-Ramos, C;Garcia-Manero, G;Down-regulation of EZH2 expression in myelodysplastic syndromesLeukemia research26970171Bone marrows from healthy individuals were obtained from AllCells (Emeryville, CA). Bone marrow mononuclear cells were isolated using a standard Ficoll gradient approach. Isolation SaveFull Paper
2016Salzano, G;Zappavigna, S;Luce, A;D'Onofrio, N;Balestrieri, ML;Grimaldi, A;Lusa, S;Ingrosso, D;Artuso, S;Porru, M;Leonetti, C;Caraglia, M;De Rosa, G;Transferrin-Targeted Nanoparticles Containing Zoledronic Acid as a Potential Tool to Inhibit Glioblastoma GrowthJournal of biomedical nanotechnology27301207Viable human bone marrow cells (purchased as frozen vials from AllCells via StemCell Technologies) were prepared with a DNase treatment (Stem Cell Technologies) and then SaveFull Paper
2016Phillips, JE;Renteria, L;Burns, L;Harris, P;Peng, R;Bauer, CM;Laine, D;Stevenson, CS;Btk Inhibitor RN983 Delivered by Dry Powder Nose-only Aerosol Inhalation Inhibits Bronchoconstriction and Pulmonary Inflammation in the Ovalbumin Allergic Mouse Model of AsthmaJournal of aerosol medicine and pulmonary drug delivery27111445Mast cells One million human cord blood derived CD34+ hematopoietic stem cells (HSCs) from different donors (AllCells #CB008F-S, Emeryville, CA) were cultured for 8 weeks in a serum-free complete medium (StemPro-34 with supplements; Invitrogen, CarlsFull Paper
2016Wang, X;Zhao, S;Su, M;Sun, L;Zhang, S;Wang, D;Liu, Z;Yuan, Y;Liu, Y;Li, Y;Geraniol improves endothelial function by inhibiting NOX-2 derived oxidative stress in high fat diet fed miceBiochemical and biophysical research communications27107694In the present study, the in vivo diet induced obese setting was reproduced in vitro by exposing HUVECs to palmitic acid (PA), the most common circulating free fatty acid, with or without geraniol. Human umbilical vein endothelial cells (HUVECs) wereFull Paper
2016Xia, T;Guan, W;Fu, J;Zou, X;Han, Y;Chen, C;Zhou, L;Zeng, C;Wang, WE;Tirofiban induces vasorelaxation of the coronary artery via an endothelium-dependent NO-cGMP signaling by activating the PI3K/Akt/eNOS pathwayBiochemical and biophysical research communications27018249Human umbilical vein endothelial cells (HUVECs) were purchased from ALLCELLS (Shanghai, China). HUVECs were cultured at 37 °C on gelatin-coated plates in the basal nutrient media MCDB-131 supplemented with 5% fetal bovine serum (FBS; Hyclone, Logan,Full Paper
2016Cao, Z;Wang, H;Mao, X;Luo, L;Noncanonical function of threonyl-tRNA synthetase regulates vascular development in zebrafishBiochemical and biophysical research communications26993167Human umbilical vein endothelial cells (HUVECs, ALLCELLS) were raised according to standard protocol. Cell immunostaining was performed as previously described [15] using SaveFull Paper
2016Sugino, N;Miura, Y;Yao, H;Iwasa, M;Fujishiro, A;Fujii, S;Hirai, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T;Early osteoinductive human bone marrow mesenchymal stromal/stem cells support an enhanced hematopoietic cell expansion with altered chemotaxis- and adhesion-related gene expression profilesBiochemical and biophysical research communications26707642Normal human BM samples that were obtained from healthy adult volunteers with informed consent were purchased from AllCells (Emeryville, CA). Human BM-MSCs were isolated and cultured based on a previously published method [3], [12], [13], [14], [15].Full Paper
2016Koestler, DC;Jones, MJ;Usset, J;Christensen, BC;Butler, RA;Kobor, MS;Wiencke, JK;Kelsey, KT;Improving cell mixture deconvolution by identifying optimal DNA methylation libraries (IDOL)BMC bioinformatics26956433Purified granulocytes, monocytes, CD4T, CD8T, natural killer cells, and B cells from normal human subjects were purchased from AllCells LLC (Emeryville, CA). As described (http://www SaveFull Paper
2016Sarkar, S;Sabhachandani, P;Stroopinsky, D;Palmer, K;Cohen, N;Rosenblatt, J;Avigan, D;Konry, T;Dynamic analysis of immune and cancer cell interactions at single cell level in microfluidic dropletsBiomicrofluidics27795747RPMI-8226 (multiple myeloma (MM) cell line) was purchased from the American Type Culture Collection (ATCC, Manassas, VA). CD8+ T cells were purchased from AllCells (#PB009-3F, AllCells, Alameda, CA). Cells were maintained in RPMI-1640 medium supplemeFull Paper
2016Kikushige, Y;Akashi, K;A TIM-3/GAL-9 autocrine stimulatory loop drives self-renewal of human myeloid leukemia stem cells and leukemic progressionExperimental HematologyHuman adult BM and PB samples were obtained from healthy donors or purchased from AllCells. CB cells were obtained from full-term deliveries (provided by the Kyushu Block Red SaveFull Paper
2016Itokazu, M;Wakitani, S;Mera, H;Tamamura, Y;Sato, Y;Takagi, M;Nakamura, H;Transplantation of Scaffold-Free Cartilage-Like Cell-Sheets Made from Human Bone Marrow Mesenchymal Stem Cells for Cartilage Repair: A Preclinical StudyCartilage27688844hBMSCs were prepared from three male donors aged 21 (#1), 27 (#2), and 31 (#3) years and from one commercially available bone marrow (Allcells LLC, Alameda, CA, USA) from a 20-year-old male (#4). The donor cells were derived from bone marrow aspirateFull Paper
2016Yan, Z;Liu, J;Xie, L;Liu, X;Zeng, Y;Role of heparan sulfate in mediating CXCL8-induced endothelial cell migrationPeerJ26870616Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells, China. HUVECs were grown in complete culture media for HUVECs (Allcells, China) in a humidified 5%/ SaveFull Paper
2016Hara, C;Kubota, S;Nishida, T;Hiasa, M;Hattori, T;Aoyama, E;Moriyama, Y;Kamioka, H;Takigawa, M;Involvement of multiple CCN family members in platelets that support regeneration of joint tissuesModern rheumatology26915735Human platelets from a healthy donor were provided by AllCells LLC (Berkley, CA). Protein extraction from the cells was performed with RIPA buffer (50 mM Tris-HCl, 0.15 M NaCl, 4 SaveFull Paper
2016Zhang, M;Jiang, L;Oxidized low-density lipoprotein decreases VEGFR2 expression in HUVECs and impairs angiogenesisExperimental and therapeutic medicine28105106Cell culture and LDL treatment In total 2 × 105 HUVECs (AllCells, LLC, Shanghai, China) were cultured for 3-5 passages in Dulbecco's modified Eagle's medium (DMEM; PromoCell GmbH, Heidelberg, Germany) supplemented with 10% fetal bovine serum (unlessFull Paper
2016Heng, BC;Zhu, S;Xu, J;Yuan, C;Gong, T;Zhang, C;Effects of decellularized matrices derived from periodontal ligament stem cells and SHED on the adhesion, proliferation and osteogenic differentiation of human dental pulp stem cells in vitroTissue & cell26796232Human DPSC (Cat No. DP003F) and SHED (Cat No. DP004F) were obtained from AllCells LLC. (Alameda, CA, USA). Human PDLSC were obtained as a gift from the School of Stomatology of Fujian Medical School (Fujian, China) and were isolated as previously desFull Paper
2016Klingelhöffer, C;Reck, A;Morsczeck, C;The extracellular concentration of osteocalcin decreased in dental follicle cell cultures during biomineralizationCytotechnology27447177Human dental follicle cells (DFCs) were purchased from ALLCells (Alameda, CA, USA). DFCs were cultivated in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich, Munich, Germany), supplemented with 10 % fetal bovine serum, FBS (Sigma-Aldrich) and 1Full Paper
2016Ahmed, HM;Luddin, N;Kannan, TP;Mokhtar, KI;Ahmad, A;Chemical analysis and biological properties of two different formulations of white portland cementsScanning26382064DPSCs cell line (AllCells, USA) obtained from biopsies of the dental pulp of adult teeth was (BCGM) (AllCells, USA), with 50 mL of human MSC stimulatory supplements (AllCells, USA). SaveFull Paper
2016Mulato, A;Hansen, D;Thielen, A;Porter, D;Stepan, G;White, K;Daeumer, M;Cihlar, T;Yant, SR;Rapid In Vitro Evaluation of Antiretroviral Barrier to Resistance at Therapeutic Drug LevelsAIDS research and human retroviruses27356854Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh leukopaks obtained from healthy volunteers under informed consent (AllCells, Inc., Alameda, CA) and SaveFull Paper
2016Kowolik, CM;Lin, M;Xie, J;Overman, LE;Horne, DA;NT1721, a novel epidithiodiketopiperazine, exhibits potent in vitro and in vivo efficacy against acute myeloid leukemiaOncotarget27863389We then tested the effect of NT1721 on primary AML samples (from AllCells, obtained from two newly diagnosed AML patients with white blood cell counts > 10 11 /L) by treating the SaveFull Paper
2016Jochems, C;Hodge, JW;Fantini, M;Fujii, R;Morillon, YM;Greiner, JW;Padget, MR;Tritsch, SR;Tsang, KY;Campbell, KS;Klingemann, H;Boissel, L;Rabizadeh, S;Soon-Shiong, P;Schlom, J;An NK cell line (haNK) expressing high levels of granzyme and engineered to express the high affinity CD16 alleleOncotarget27861156um iodide (AO/PI) staining on a Cellometer Auto 2000 (Nexcelom, San Mateo, CA), according to the manufacturer's instructions. Release of IL-2 into culture supernatants was measured with an ELISA kit (AllCells, Alameda, CA). Antibodies and flow cytomFull Paper
2016Gołąb, K;Grose, R;Trzonkowski, P;Wickrema, A;Tibudan, M;Marek-Trzonkowska, N;Matosz, S;Solomina, J;Ostrega, D;Michael Millis, J;Witkowski, P;Utilization of leukapheresis and CD4 positive selection in Treg isolation and the ex-vivo expansion for a clinical application in transplantation and autoimmune disordersOncotarget27821811We obtained de-identified human cell leukapheresis product for the all experiments from the commercial vendor AllCells, Alameda, CA, USA, therefore the study was exempt from the SaveFull Paper
2016Yang, H;Sun, J;Wang, F;Li, Y;Bi, J;Qu, T;Umbilical cord-derived mesenchymal stem cells reversed the suppressive deficiency of T regulatory cells from peripheral blood of patients with multiple sclerosis in a co-culture - a preliminary studyOncotarget27705922RRMS, n=12, female=8, male=4), mean age 53.75 years old (49 years to 64 years old) and healthy donors (n=10, female=4, male=6), mean age 28.38 years old (22 years to 39 years old) were purchased from AllCells Inc (Emeryville, CA) or StemExpress (PlacFull Paper
2016Xu, Y;Wang, K;Yu, Q;FRMD6 inhibits human glioblastoma growth and progression by negatively regulating activity of receptor tyrosine kinasesOncotarget27661120entific. Secondary anti-rabbit Alexa Fluor 594 and anti-mouse Alexa Fluor 488 were from Invitrogen. Cells, GBM cell lines, and primary GBM cells Normal human astrocytes (NHAs) were obtained from ALLCELLS, Inc and ScienCell. SF126, SF188, SF210, SFFull Paper
2016Ferluga, S;Tomé, CM;Herpai, DM;D'Agostino, R;Debinski, W;Simultaneous targeting of Eph receptors in glioblastomaOncotarget27494882r Center of Wake Forest University). Stacked 2D images were obtained in some cases. Images were processed with OlyVIA V2.8. Dendritic cells Myeloid-derived naïve dendritic cells were purchased from AllCells LLC (Alameda, CA). Cells were grown in RPMFull Paper
2016Sumbayev, VV;Gonçalves Silva, I;Blackburn, J;Gibbs, BF;Yasinska, IM;Garrett, MD;Tonevitsky, AG;Ushkaryov, YA;Expression of functional neuronal receptor latrophilin 1 in human acute myeloid leukaemia cellsOncotarget27322212oetal calf serum, penicillin (50 IU/ml) and streptomycin sulphate (50 μg/ml). Primary human AML cells Primary human AML mononuclear cells (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordanFull Paper
2016Wang, Y;Zhan, Z;Jiang, X;Peng, X;Shen, Y;Chen, F;Ji, Y;Liu, W;Shi, Y;Duan, W;Ding, J;Ai, J;Geng, M;Simm530, a novel and highly selective c-Met inhibitor, blocks c-Met-stimulated signaling and neoplastic activitiesOncotarget27191264rom Shanghai Cancer Institute, Renji Hospital, Shanghai Jiaotong University School of Medicine (Shanghai, China). SK-OV-3 was obtained from Fudan University (Shanghai, China). HUVEC was obtained from AllCells Bio (Shanghai, China). Cells were routineFull Paper
2016Ye, BG;Sun, HC;Zhu, XD;Chai, ZT;Zhang, YY;Ao, JY;Cai, H;Ma, DN;Wang, CH;Qin, CD;Gao, DM;Tang, ZY;Reduced expression of CD109 in tumor-associated endothelial cells promotes tumor progression by paracrine interleukin-8 in hepatocellular carcinomaOncotarget27121053(Sigma-Aldrich, St. Louis, MO, USA) in a humidified atmosphere of 5% CO2 at 37°C. Human normal liver L-02 cells (Chinese Academy of Sciences) were cultured under the same conditions. Primary HUVEC (AllCells, Shanghai, China) were cultured in endothelFull Paper
2016Schwaederle, M;Husain, H;Fanta, PT;Piccioni, DE;Kesari, S;Schwab, RB;Banks, KC;Lanman, RB;Talasaz, A;Parker, BA;Kurzrock, R;Detection rate of actionable mutations in diverse cancers using a biopsy-free (blood) circulating tumor cell DNA assayOncotarget26848768yzed for 54 genes via NGS in circulating tumor, cell-free DNA. RESULTS Analysis of control samples from healthy persons During the technology development process, 79 healthy normal controls (source: AllCells, http://www.allcells.com) were tested andFull Paper
2016Li, Y;Wang, Y;Li, L;Kong, R;Pan, S;Ji, L;Liu, H;Chen, H;Sun, B;Hyperoside induces apoptosis and inhibits growth in pancreatic cancer via Bcl-2 family and NF-κB signaling pathway both in vitro and in vivoTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine26676634Human umbilical vein endothelial cell line (HUVEC) was obtained from all cells (Shanghai, China) and were cultured in complete medium (HUVEC-004, supplied by AllCells). All cells SaveFull Paper
2016Aizman, I;Holland, WS;Yang, C;Bates, D;αSMA Expression in Large Colonies of Colony-Forming Units-Fibroblast as an Early Predictor of Bone Marrow MSC ExpandabilityCell medicine28003933The three lots that were produced from BM aspirates obtained at AllCells and, coincidentally, from older donors (see Materials and Methods) were all in the slow-growing group; however SaveFull Paper
2016Liu, W;Lu, Y;Chai, X;Liu, X;Zhu, T;Wu, X;Fang, Y;Liu, X;Zhang, X;Antitumor activity of TY-011 against gastric cancer by inhibiting Aurora A, Aurora B and VEGFR2 kinasesJournal of experimental & clinical cancer research : CR27887633Human umbilical vein endothelial cell (HUVEC) obtained from Allcells (Emeryville, CA, USA) were kept in HUVEC basic medium supplemented with 10% FBS (Allcells, Emeryville, CA, SaveFull Paper
2016Han, X;Medeiros, LJ;Zhang, YH;You, MJ;Andreeff, M;Konopleva, M;Bueso-Ramos, CE;High Expression of Human Homologue of Murine Double Minute 4 and the Short Splicing Variant, HDM4-S, in Bone Marrow in Patients With Acute Myeloid Leukemia or Myelodysplastic SyndromeClinical lymphoma, myeloma & leukemia27155969cDNAs from purified normal CD33+ bone marrow myeloid cells, CD34+ hematopoietic progenitor cells, CD71+ normoblasts, and CD19+ circulating peripheral blood B cells were purchased from AllCells, LLC (Emeryville, CA) for reverse transcriptase-polymerasFull Paper
2016Ali, S;Cell Line Cross-Contamination and Accidental Co-CultureJournal of Stem Cell Research & Therapeuticsa https://www.abmgood.com/ Custom-Cell-Authentication-Service.html STR Profiling Analytical Biological Services Inc, USA http://www.absbio.com/products /cellQC/celllineauthen.html STR profiling AllCells, California www.allcells.com - AsteranFull Paper
2016Dou, W;Cheung, S;Gao, C;Xu, C;Xu, L;Wei, J;Detecting table clones and smells in spreadsheetsProceedings of the 2016 24th ACM SIGSOFT International Symposium on Foundations of Software EngineeringTableCheck's clone detection algorithm (Algorithm 1) takes the lookup table and all cells (allCells) Then all cells in this group are removed from allCells (Lines 14-18). (5) The algorithm SaveFull Paper
2016DeWitt, M;Magis, W;Bray, N;Wang, T;Berman, J;Urbinati, F;Muñoz, D;Kohn, D;Walters, M;Carroll, D;Martin, D;Corn, J;Efficient Correction of the Sickle Mutation in Human Hematopoietic Stem Cells Using a Cas9 Ribonucleoprotein ComplexbioRxivCryopreserved WT human mobilized peripheral blood CD34+ HSPCs were purchased from Allcells, Inc. SCD CD34+ HSPCs were prepared by Allcells Inc. from whole blood obtained SaveFull Paper
2016Meader, ES;Understanding the role of miRNA expression in the differentiation of haematopoietic stem cells from pluripotent stem cellsThesisnot been released in spite of several requests, but the sequences of the miRNAs tested are shown in Table 2.4. Small nuclear RNAs RNU44 and RNU48 were chosen as internal controls as they are known to be stably and abundantly expressed across differenFull Paper
2016Tran, R;A microfluidics-based paradigm for clinical lentivector gene transferThesisFrozen human pan T cells were purchased from AllCells (AllCells, LLC., Alameda, CA, USA). Cells were thawed following the AllCells thawing protocol and allowed to recover in RPMI 1640 media supplemented with 10% FBS, L-Glutamine, 25 mM HEPES, and 1%Full Paper
2016Abu Samra, DBK;Molecular Characterization of the Interactions between Vascular Selectins and Glycoprotein Ligands on Human Hematopoietic Stem/Progenitor CellsThesisbone marrow (CD34+ -bone marrow), AML derived bone marrow (from patients with newly diagnosed or relapsed AML; AML bone marrow-CD34+ ) or the mononuclear fraction of whole bone marrow were purchased from ALL CELLS (Alameda, CA, USA; www.allcells.com)Full Paper
2016Ma, L;Targeting Drug Resistance in Chronic Myeloid Leukemia: A DissertationThesisin FBS + 10% DMSO, frozen slowly overnight, and then transferred to liquid nitrogen for long-term storage. All samples were provided in a de-identified manner. Samples from normal donors were purchased from a commercial vendor (AllCells or Lonza)Full Paper
2016Liu, X;Functional and structural study of the AHI-1 SH3 domain, characterization of the BCR-ABL-AHI-1-Dynamin-2 protein complex and investigation of oncogenic roles of dynamin-2 in chronic myeloid leukemiaThesisPrimary CML samples were obtained from newly-diagnosed CP-CML patients before any TKI intervention, and BM cells were obtained from health donors (ALLCELLS, Alameda, CA). The subsequent IM-responders were classified based on the European Leukemia NetFull Paper
2016Ferreira, L;Transcriptional Control of Maternal-Fetal Immune ToleranceThesisPrimary CD4+ T cells were isolated from peripheral blood (Leukopacs, MGH) using RosetteSep CD4+ T cell enrichment cocktail (STEMCELL Technologies). CD34+ cells from G-CSF mobilized peripheral blood were purchased from AllCellsFull Paper
2016Coronado, RE;The effect of decellularized liver matrix on stem cell differentiation into hepatocyte-like cellsThesisBone Marrow Derived Mesenchymal Stromal/Stem Cells: Bone marrow derived mesenchymal stem cells (BM-MSC) were isolated from whole bone marrow aspirates purchased from AllCells Alameda, CA from donors less than 30 years old. The isolation protocol wasFull Paper
2016Gangolli, R;A novel biomimetic scaffold for guided tissue regeneration of the pulp-dentin complexThesisDPSC from AllCells at Passage P5 were used for the study. DPSC-P5 were cultured on T175 flasks using proliferation media with MEM Alpha, 15% FBS and 1% Pen-strep which had been filtered through a 0.2 um filter flask. Culture conditions were maintaineFull Paper
2016Bjornson-Hooper, ZB;Cross-Species Immune System AtlasThesisVenous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human subjects research) or from volunteers from the Stanford community under an IRB-approved protocol. Baboon (Papio hamadryas anubis x Papio haFull Paper
2016Varma, D;Development of a Redox-Crosslinked Cellulose-Based Hydrogel for Nucleus Pulposus Replacement and RepairThesishMSCs were isolated from adult human bone marrow (AllCells, Emeryville, CA) via enriched density gradient centrifugation as previously described (Gupta et al., 2011; Gupta and Nicoll, 2014b). Briefly, an hMSC Enrichment Cocktail (StemCell TechnologieFull Paper
2016Sivajothi, SK;Comparative properties of stromal cells and their role in tissue regenerationThesis2.1 Apheresis Products Non-mobilized PBPC and G-CSF mobilized PBPC were purchased from Allcells LLC (Emeryville, CA): 2.2 Cells and Cell Culture Human bone marrow MSCs were isolated by differential adhesion from bonemarrow aspirate obtained from humaFull Paper
2016Fragiadakis, GK;Profiling Human Immune Variation at the Systems-Level in Clinical Contexts and at Steady-StateThesisBlood Venous human blood was obtained from the Stanford Blood Center, AllCells Inc. (Alameda, CA) (exempt, non-human subjects research) or from volunteers from the Stanford community under an IRB-approved protocol. Antibodies Purified antibodies wereFull Paper
2016Fried, ES;Applications of Proteolipobeads in Biomimetic Cellular Interactions And Lipid Phase SeparationThesisN-Diisopropylethylamine (DIEA), piperidine, carboxymethylcellulose (CMC), and methacrylic anhydride were purchased from Sigma Aldrich (St. Louis, MO). Human mesenchymal stem cells (MSCs) isolated from bone marrow extracted from a 24-year-old male werFull Paper
2016Lucera, MB;Lysine Acetylation and Small Molecule Epigenetic Inhibition Reveal Novel Mechanisms Controlling Cellular Susceptibility to HIV-1 InfectionThesisCD4+ T cells were obtained by leukopheresis from ALLCELLS, LLC. All subjects were healthy control donors negative for HBV, HCV, and HIV. PBMCs were isolated by ficoll gradient separation while CD4+ T cells were purified by adding RosetteSep CD4+ T ceFull Paper
2016Milford, TA;Comparative Studies of TSLP and IL-7 in Normal Early Human Neonatal and Adult B Cell DevelopmentThesisCB was collected according to guidelines approved by the Institutional Review Boards at Children’s Hospital Los Angeles and Loma Linda University. Adult human BM was purchased from AllCells, LLC, Berkeley, CA; the National Disease Research InterchangFull Paper
2016Richter, M;Saydaminova, K;Yumul, R;Krishnan, R;Liu, J;Nagy, EE;Singh, M;Izsvák, Z;Cattaneo, R;Uckert, W;Palmer, D;Ng, P;Haworth, KG;Kiem, HP;Ehrhardt, A;Papayannopoulou, T;Lieber, A;In vivo transduction of primitive mobilized hematopoietic stem cells after intravenous injection of integrating adenovirus vectorsBlood27554082Human CD34+-enriched cells from G-CSF mobilized normal donors were obtained from the Fred Hutchinson Cancer Research Center Cell Processing Core Facility and cultured as described previously.8 Colony-forming unit (CFU) assays were performed using ColFull Paper
2016Castillo, J;Raisner, R;Gascoigne, K;Dos Santos, G;Clarke, E;Huang, A;Lackner, M;Mounir, Z;Abstract 4516: Evaluating the chromatin state as a predictive biomarker for BET inhibitor sensitivity in hematological malignanciesCancer Research1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++2ReachBio, WA.++2ReachBio, WA.++1Genentech, South San Francisco, CA;++1Genentech, South San Francisco, CA;++1Genentech, South San FranciFull Paper
2016Ferguson, GD;Delgado, M;Plantevin-Krenitsky, V;Jensen-Pergakes, K;Bates, RJ;Torres, S;Celeridad, M;Brown, H;Burnett, K;Nadolny, L;Tehrani, L;Packard, G;Pagarigan, B;Haelewyn, J;Nguyen, T;Xu, L;Tang, Y;Hickman, M;Baculi, F;Pierce, S;Miyazawa, K;Jackson, P;Chamberlain, P;LeBrun, L;Xie, W;Bennett, B;Blease, K;A Novel Triazolopyridine-Based Spleen Tyrosine Kinase Inhibitor That Arrests Joint InflammationPloS one26756335ature in the dark. Fluorescence readings at 460 nm and 530 nm were taken using the Flexstation II (Molecular Devices). Myeloid colony forming assays to assess Jak2 cellular activity were conducted at ReachBio (Seattle, WA). Ramos NFAT β-lactamase reFull Paper
2016Hariharan, S;STUDY OF GROWTH OF STRESS ERYTHROID PROGENITORS FROM PERIPHERAL BLOOD MONONUCLEAR CELLS IN MURINE AND HUMAN ANEMIC MODEL SYSTEMSThesisThe control Peripheral Blood samples were obtained from ReachBio Labs. The PBMCs were then isolated from peripheral blood using Histopaque 1077 using density gradient. The SaveFull Paper
2016Holzapfel, N;Investigating the Role of the RNA-Binding Protein MUSASHI-2 (MSI2) in Normal Hematopoiesis and LeukemiaThesisAML*CFU%assays%were%performed%in%semi*solid%ColonyGel%media%(Human%Complete% Media% 1102;% reachBio)% with% flow*sorted% GFP+%MSI2% knockdown% AML% cells.% 10000% GFP+%cells%were%plated%in%duplicate%in%each%well%of%a%24*well%plate%and%loose%colonies%Full Paper
2015Sun, LL;Ellerman, D;Mathieu, M;Hristopoulos, M;Chen, X;Li, Y;Yan, X;Clark, R;Reyes, A;Stefanich, E;Mai, E;Young, J;Johnson, C;Huseni, M;Wang, X;Chen, Y;Wang, P;Wang, H;Dybdal, N;Chu, YW;Chiorazzi, N;Scheer, JM;Junttila, T;Totpal, K;Dennis, MS;Ebens, AJ;Anti-CD20/CD3 T cell-dependent bispecific antibody for the treatment of B cell malignanciesSci Transl Med25972002We have obtained 40 ml of whole blood collected in Streck tubes, separated into plasma and extracted cfRNA samples from two Stage IV Prostate cancer patients and three healthy controls from Conversant Bio and generated cfRNA sequencing data with UMIsFull Paper
2015Li, L;Lyu, X;Hou, C;Takenaka, N;Nguyen, HQ;Ong, CT;Cubeñas-Potts, C;Hu, M;Lei, EP;Bosco, G;Qin, ZS;Corces, VG;Widespread rearrangement of 3D chromatin organization underlies polycomb-mediated stress-induced silencingMol. Cell25818644We thank Drs. John T. Lis for providing antibodies against RNAPII, and Vincent Pirrotta and Richard Jones for providing antibodies against Pc. We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology, and specialFull Paper
2015Bhate, A;Parker, DJ;Bebee, TW;Ahn, J;Arif, W;Rashan, EH;Chorghade, S;Chau, A;Lee, JH;Anakk, S;Carstens, RP;Xiao, X;Kalsotra, A;ESRP2 controls an adult splicing programme in hepatocytes to support postnatal liver maturationNat Commun26531099... RI digested amplified PCR products for Esrp2 cDNA in pDP19 vector (Ambion). Sense and anti-sense RNA strands were generated as previously described43. In situ hybridization for Esrp2 was performed by Phylogeny Inc. (Columbus, Ohio.) The C57BL/6 mFull Paper
2015Weckselblatt, B;Hermetz, KE;Rudd, MK;Unbalanced translocations arise from diverse mutational mechanisms including chromothripsisGenome Res.26070663We used Agilent SureSelect Target Enrichment to pull down 40-kb regions around breakpoints fine-mapped by custom array CGH. SureSelect followed by Illumina HiSeq sequencing was performed at HudsonAlpha Genomic Services Laboratory, and sequence analysFull Paper
2015Jagannathan, S;Abdel-Malek, MA;Malek, E;Vad, N;Latif, T;Anderson, KC;Driscoll, JJ;Pharmacologic screens reveal metformin that suppresses GRP78-dependent autophagy to enhance the anti-myeloma effect of bortezomibLeukemia26108695Eagle's medium. Bone marrow aspirates were obtained after approval institutional review board or from ConversantBio (Huntsville, AL, USA) and CD138 + cells then isolated (Miltenyi Biotec, San Diego, CA, USA). Side populationFull Paper
2015Jagannathan, S;Vad, N;Vallabhapurapu, S;Vallabhapurapu, S;Anderson, KC;Driscoll, JJ;MiR-29b replacement inhibits proteasomes and disrupts aggresome+autophagosome formation to enhance the antimyeloma benefit of bortezomibLeukemia25234165(c) Effect of control or miR-29b on myeloma patient tumor cells. CD138 + PCs from bone marrow (BM; ConversantBio, Huntsville, AL, USA) were isolated using microbead-positive selection (Miltenyi Biotec, Auburn, CA, USA)Full Paper
2015Blume-Jensen, P;Berman, DM;Rimm, DL;Shipitsin, M;Putzi, M;Nifong, TP;Small, C;Choudhury, S;Capela, T;Coupal, L;Ernst, C;Hurley, A;Kaprelyants, A;Chang, H;Giladi, E;Nardone, J;Dunyak, J;Loda, M;Klein, EA;Magi-Galluzzi, C;Latour, M;Epstein, JI;Kantoff, P;Saad, F;Development and clinical validation of an in situ biopsy-based multimarker assay for risk stratification in prostate cancerClin. Cancer Res.25733599To develop a robust assay, multiple institutions were recruited representing typical North American patient cohorts (Table 1): Urology Austin, Chesapeake Urology Associates, Cleveland Clinic, Michigan Urology, and Folio Biosciences. Biopsy sample incFull Paper
2015Garcia-Manero, G;Khoury, HJ;Jabbour, E;Lancet, J;Winski, SL;Cable, L;Rush, S;Maloney, L;Hogeland, G;Ptaszynski, M;Calvo, MC;Bohannan, Z;List, A;Kantarjian, H;Komrokji, R;A phase I study of oral ARRY-614, a p38 MAPK/Tie2 dual inhibitor, in patients with low or intermediate-1 risk myelodysplastic syndromesClin. Cancer Res.25480830... s (Aurora, CO) proprietary CellMap customized algorithm for p-p38 and CC3, respectively. Age-defined FFPE bone marrow biopsies from apparently healthy subjects (60–74 years old) were obtained from Folio Biosciences (Columbus, OH). Plasma was isoFull Paper
2015Grabek, KR;Diniz Behn, C;Barsh, GS;Hesselberth, JR;Martin, SL;Enhanced stability and polyadenylation of select mRNAs support rapid thermogenesis in the brown fat of a hibernatorElife25626169Department of Cell and Developmental Biology, University of Colorado School of Medicine, Aurora, United States.++Department of Applied Math and Statistics, Colorado School of Mines, Golden, United States.++Department of Research, HudsonAlpha InstitutFull Paper
2015Li, C;Gowan, S;Anil, A;Beck, BH;Thongda, W;Kucuktas, H;Kaltenboeck, L;Peatman, E;Discovery and validation of gene-linked diagnostic SNP markers for assessing hybridization between Largemouth bass (Micropterus salmoides) and Florida bass (M. floridanus)Mol Ecol Resour25047482; of 110-140 nm. After KAPA quantitation and dilution, based on included DNA standards (1-6), the libraries were sequenced in a single lane on an Illumina HiSeq 2000 instrument with 100 bp paired‐end (PE) reads at HudsonAlpha Genomic Services Lab (HFull Paper
2015Nguyen, D;Rubinstein, L;Takebe, N;Miele, L;Tomaszewski, JE;Ivy, P;Doroshow, JH;Yang, SX;Notch1 phenotype and clinical stage progression in non-small cell lung cancerJ Hematol Oncol25653136Lung cancer tissue microarrays and clinicopathological data were obtained from Cybrdi, Incorporation (Rockville, MD), and FOLIO Biosciences (Powell, OH). Approval of the biomarker study on de-identified human tissues was obtained from the Office of HFull Paper
2015Huynh, H;Hao, HX;Chan, SL;Chen, D;Ong, R;Soo, KC;Pochanard, P;Yang, D;Ruddy, D;Liu, M;Derti, A;Balak, MN;Palmer, MR;Wang, Y;Lee, BH;Sellami, D;Zhu, AX;Schlegel, R;Huang, A;Loss of Tuberous Sclerosis Complex 2 (TSC2) Is Frequent in Hepatocellular Carcinoma and Predicts Response to mTORC1 Inhibitor EverolimusMol. Cancer Ther.25724664HCC cell lines and patient-derived xenografts were collected, fixed with formalin and processed for paraffin embedding. HCC tissue microarrays were purchased from BioChain (catalog # Z7020056 and Z7020057, unrelated HCC TMAs) and FolioBio (catalog# AFull Paper
2015Mezache, L;Paniccia, B;Nyinawabera, A;Nuovo, GJ;Enhanced expression of PD L1 in cervical intraepithelial neoplasia and cervical cancersMod. Pathol.26403783and cervical cancers Louisa Mezache 1 Bernard Paniccia 1 Angelique Nyinawabera 1 Gerard Nuovo 1,2 Phylogeny Inc. 1 , Powell, Ohio and OSU Comprehensive Cancer Center 2 , Columbus Ohio Page 2. 2 - Mezache et al Direct all correspondence to: Gerard J NFull Paper
2015Shen, J;Putt, KS;Visscher, DW;Murphy, L;Cohen, C;Assessment of folate receptor-β expression in human neoplastic tissuesOncotarget... from US Biomax (Rockville, MD), and v) a breast tumor microarray (ARY-HH0056), a brain glioma tumor microarray (ARY-HH0138) and an endometrial carcinoma progression tumor microarray (ARY-HH0211) from Folio Biosciences (Columbus, OH). Oregon greenFull Paper
2015Azad, AK;Rajaram, MV;Metz, WL;Cope, FO;Blue, MS;Vera, DR;Schlesinger, LS;γ-Tilmanocept, a New Radiopharmaceutical Tracer for Cancer Sentinel Lymph Nodes, Binds to the Mannose Receptor (CD206)J. Immunol.26202986We thank Phylogeny, Inc. (Powell, OH) for sectioning and processing the lymph node tissues. We also acknowledge the support of the Campus Microscopy and Imaging Facility of The Ohio State University (Columbus, OH) for confocal microscopy.Full Paper
2015Waight, JD;Takai, S;Marelli, B;Qin, G;Hance, KW;Zhang, D;Tighe, R;Lan, Y;Lo, KM;Sabzevari, H;Hofmeister, R;Wilson, NS;Cutting edge: epigenetic regulation of Foxp3 defines a stable population of CD4+ regulatory T cells in tumors from mice and humansJ. Immunol.25548231Mouse tumor cells were isolated as described previously (12). Human tumor specimens were collected before therapy and purchased from Conversant Bio. Healthy donor PBMCs (Research Blood Components) were isolated using Ficoll-Paque density gradient. SaFull Paper
2015Li, Y;Kocot, KM;Schander, C;Santos, SR;Thornhill, DJ;Halanych, KM;Mitogenomics reveals phylogeny and repeated motifs in control regions of the deep-sea family Siboglinidae (Annelida)Mol. Phylogenet. Evol.25721539Mitogenomics reveals phylogeny and repeated motifs in control regions of the deep-sea family Siboglinidae (Annelida)Full Paper
2015Cheng, A;Hernandez, N;Li, M;San Jose Hinahon, C;Magdaleno, S;Abstract 4755: Maximize genomic analysis through dual recovery of DNA and RNA from the same clinical sampleMolecular and Cellular Biologyvariant, indel data, RNA fusion detection and expression data. MATERIALS AND METHODS FFPE surgical resection and FNA blocks were purchased from Folio Biosciences. Age of blocks ranged from 1992- 2011 with various tumor stages. One 4um section mountedFull Paper
2015Cunningham, CB;Ji, L;Wiberg, RA;Shelton, J;McKinney, EC;Parker, DJ;Meagher, RB;Benowitz, KM;Roy-Zokan, EM;Ritchie, MG;Brown, SJ;Schmitz, RJ;Moore, AJ;The Genome and Methylome of a Beetle with Complex Social Behavior, Nicrophorus vespilloides (Coleoptera: Silphidae)Genome Biol Evol26454014Testing for conserved and novel mechanisms underlying phenotypic evolution requires a diversity of genomes available for comparison spanning multiple independent lineages. For example, complex social behavior in insects has been investigated primarilFull Paper
2015Yamaguchi, SI;Ueki, A;Sugihara, E;Onishi, N;Yaguchi, T;Kawakami, Y;Horiuchi, K;Morioka, H;Matsumoto, M;Nakamura, M;Muto, A;Toyama, Y;Saya, H;Shimizu, T;Synergistic antiproliferative effect of imatinib and adriamycin in platelet-derived growth factor receptor-expressing osteosarcoma cellsCancer Sci.25940371A lung tissue section was randomly obtained from each lobe. Human OS tissue array. An array of human OS specimens was obtained from Folio Biosciences (Powell, OH, USA) and was subjected to immunohistochemical staining for PDGFRβFull Paper
2015Zemskova, MY;Song, JH;Cen, B;Cerda-Infante, J;Montecinos, VP;Kraft, AS;Regulation of prostate stromal fibroblasts by the PIM1 protein kinaseCell. Signal.25451079scores. P values were calculated by t test and represent the probability of no difference between PIM1 positive cells in the stroma of normal and cancer tissues. (C) IHC staining of PIM1 using a TMA (Folio Bioscience). PIM1Full Paper
2015Ergin, A;Großerüschkamp, F;Theisen, O;Gerwert, K;Remiszewski, S;Thompson, CM;Diem, M;A method for the comparison of multi-platform spectral histopathology (SHP) data setsAnalyst25664352M. Miljković, et al., Spectral Cytopathology: new aspects of data collection, manipulation and confounding effects, Analyst, 2013, 138, 3975-3982 RSC . foliobio.com. Deparaffin Protocol. Available from: http://foliobio.com/media/pdf/Deparaffin%20protFull Paper
2015Steffen, MM;Belisle, BS;Watson, SB;Boyer, GL;Bourbonniere, RA;Wilhelm, SW;Metatranscriptomic evidence for co-occurring top-down and bottom-up controls on toxic cyanobacterial communitiesAppl. Environ. Microbiol.25662977... using the Epicentre Ribo-Zero rRNA removal kit (Epicentre, Madison, WI, USA). Secondary quality analysis and sequencing were done on the Illumina HiSeq platform for 50-bp paired-end sequencing by the HudsonAlpha Genomic Services Laboratory (HuntsFull Paper
2015Van Bortle, K;Peterson, AJ;Takenaka, N;O'Connor, MB;Corces, VG;CTCF-dependent co-localization of canonical Smad signaling factors at architectural protein binding sites in D. melanogasterCell Cycle26125535llumina adaptors (Illumina Cat# PE-102-1001) were titrated according to prepared DNA ChIP sample concentration and ligated with T4 ligase (NEB Cat# M0202S). Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (NEFull Paper
2015Ritchie, SA;Chitou, B;Zheng, Q;Jayasinghe, D;Jin, W;Mochizuki, A;Goodenowe, DB;Pancreatic cancer serum biomarker PC-594: Diagnostic performance and comparison to CA19-9World J. Gastroenterol.26074698MATERIALS AND METHODS. Sample cohort. PaC (n = 84) and control (n = 99) serum samples were obtained from Conversant Bio (www.conversantbio.com). All patients signed informed consents, and samples were collectedFull Paper
2015Premkumar, DR;Jane, EP;Pollack, IF;Cucurbitacin-I inhibits Aurora kinase A, Aurora kinase B and survivin, induces defects in cell cycle progression and promotes ABT-737-induced cell death in a caspase-independent manner in malignant human glioma cellsCancer Biol. Ther.25482928We also obtained primary glioma cells from Conversant Biologics (Huntsville, AL). Established malig- nant human glioma cell lines such as T98G, A172 and LN229 were obtained from the American Type Culture Collection (Manassas, VA)Full Paper
2015Tehranchian, P;Norsworthy, JK;Nandula, V;McElroy, S;Chen, S;Scott, RC;First report of resistance to acetolactate-synthase-inhibiting herbicides in yellow nutsedge (Cyperus esculentus): confirmation and characterizationPest Manag. Sci.25307777To identify potential target-site mutations, massively parallel sequencing using the Illumina HiSeq (Illumina, San Diego, CA; http://www.illumina.com/) platform was utilized in lieu of traditional short-read capillary sequencing using the polymeraseFull Paper
2015Campos, AH;Schreeder, M;Parry-Jones, A;Abdelhafiz, AS;Larson, D;Pruetz, B;Geddes, T;Salman, A;Lazaris, A;Addressing the Challenge of Financial Sustainability in BiobankingBiopreserv Biobank26697907Marshall Schreeder. Chief Executive Officer, Co-Founder, Conversant Bio, Huntsville, Alabama Conversant Bio, Huntsville, Alabama. Conversant Bio was founded in 2006 because the founders knew that the drug discovery research model was brokenFull Paper
2015Chen, S;McElroy, JS;Flessner, ML;Dane, F;Utilizing next-generation sequencing to study homeologous polymorphisms and herbicide-resistance-endowing mutations in Poa annua acetolactate synthase genesPest Manag. Sci.25180862Detection of single nucleotide polymorphisms (SNPs) related to herbicide resistance in non-model polyploid weed species is fraught with difficulty owing to the gene duplication and lack of reference sequences. Our research seeks to overcome these obsFull Paper
2015Cui, J;Xu, J;Zhang, S;Wang, K;Jiang, Y;Mahboob, S;Al-Ghanim, KA;Xu, P;Transcriptional Profiling Reveals Differential Gene Expression of Amur Ide (Leuciscus waleckii) during Spawning MigrationInt J Mol Sci26096003... nt Technologies, Santa Clara, CA, USA). Equal amounts of high quality RNA samples from the liver were then pooled for RNA-Seq analysis. RNA-Seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2015Baker, GM;Murphy, T;Block, T;Nguyen, D;Lynch, FJ;Development and validation of an immunohistochemistry assay to assess glucocorticoid receptor expression for clinical trials of mifepristone in breast cancerCancer Manag Res26673410Tiffany Murphy. 2 QualTek Molecular Laboratories, Newtown, PA, USA. Find articles by Tiffany Murphy. Thaddeus Block Dat Nguyen. 3 Corcept Therapeutics, Menlo Park, CA, USA. Find articles by Dat Nguyen. Frank J Lynch. 2 QualTek Molecular LaboratorieFull Paper
2015Zisis, K;Piper, M;Henderson, MK;The 2015 ISBER Annual Meeting & Exhibits in Phoenix, Arizona, USA: bridging the canyon-connecting biobank communities through innovations in global health, research and environmental preservationBiopreserv Biobank25686051This year's session focused on the business aspects of biobank planning, and included Marshall Schreeder's (ConversantBIO) perspective from a commer- cial biobank and Clive Green's (AstraZeneca) business planning for a pharmaceutical biobankFull Paper
2015Zhao, H;Li, C;Beck, BH;Zhang, R;Thongda, W;Davis, DA;Peatman, E;Impact of feed additives on surface mucosal health and columnaris susceptibility in channel catfish fingerlings, Ictalurus punctatusFish Shellfish Immunol.26164837RNA quality of each sample was measured on an Agilent 2100 Bioanalyzer using the RNA Nano Bioanalysis chip. RNA-seq library preparation was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA)Full Paper
2015Zhu, J;Li, C;Ao, Q;Tan, Y;Luo, Y;Guo, Y;Lan, G;Jiang, H;Gan, X;Trancriptomic profiling revealed the signatures of acute immune response in tilapia (Oreochromis niloticus) following Streptococcus iniae challengeFish Shellfish Immunol.26117728; For each timepoint, equal amounts of RNA from the three replicates were pooled for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA)Full Paper
2015Foster, KA;Jane, EP;Premkumar, DR;Morales, A;Pollack, IF;NVP-BKM120 potentiates apoptosis in tumor necrosis factor-related apoptosis-inducing ligand-resistant glioma cell lines via upregulation of Noxa and death receptor 5Int. J. Oncol.26044191U87 was obtained from American Type Culture Collection (Manasas, VA, USA) and LNZ308 was kindly provided by Dr Nicolas de Tribolet (Switzerland). We obtained primary GBM cells from Conversant Biologics (Huntsville, AL, USA)Full Paper
2015Baxter, A;Thain, S;Banerjee, A;Haswell, L;Parmar, A;Phillips, G;Minet, E;Targeted omics analyses, and metabolic enzyme activity assays demonstrate maintenance of key mucociliary characteristics in long term cultures of reconstituted human airway epitheliaToxicol In Vitro25863282The 180 PBS was collected and stored at A80 °C. Sputum from 10 non- 181 smokers (Sofia Bio, Sofia, Bulgaria, study code SB-SC_v1) and 182 MucilAir™ ASL were thawed, centrifuged and filtered using a 183 0.2 lm filter plate (PALL Corporation, Port WashFull Paper
2015Marshall, DC;Lyman, SK;McCauley, S;Kovalenko, M;Spangler, R;Liu, C;Lee, M;O'Sullivan, C;Barry-Hamilton, V;Ghermazien, H;Mikels-Vigdal, A;Garcia, CA;Jorgensen, B;Velayo, AC;Wang, R;Adamkewicz, JI;Smith, V;Selective Allosteric Inhibition of MMP9 Is Efficacious in Preclinical Models of Ulcerative Colitis and Colorectal CancerPLoS ONE25961845... h as CRC. Materials and Methods Tissues Fresh-frozen (FF) and formalin fixed and paraffin embedded (FFPE) human tissues were obtained from Cureline, Inc. (Burlingame, CA), Asterand (Detroit, MI), or Folio Biosciences (Powell, OH). Recombinant MFull Paper
2015Hoek, KL;Samir, P;Howard, LM;Niu, X;Prasad, N;Galassie, A;Liu, Q;Allos, TM;Floyd, KA;Guo, Y;Shyr, Y;Levy, SE;Joyce, S;Edwards, KM;Link, AJ;A cell-based systems biology assessment of human blood to monitor immune responses after influenza vaccinationPLoS ONE25706537Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, TN, 37232, United States of America.++Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, 37232, United StatesFull Paper
2015Kalinsky, K;Mayer, JA;Xu, X;Pham, T;Wong, KL;Villarin, E;Pircher, TJ;Brown, M;Maurer, MA;Bischoff, FZ;Correlation of hormone receptor status between circulating tumor cells, primary tumor, and metastasis in breast cancer patientsClin Transl Oncol25613123Peripheral blood was collected under appropriate third party institution review board approved protocols (Columbia University Medical Center, AdeptBio, ConversantBIO and BioOptions) and delivered to Biocept's CLIA/CAP accredited laboratoryFull Paper
2015Wang, Z;Lebron, JA;Wolf, JJ;Reliable quantification of mRNA in archived formalin-fixed tissue with or without paraffin embeddingJ Pharmacol Toxicol Methods25280797quantify mRNA in archived FFPE and prolonged formalin-fixed tissue. 2. Methods. 2.1. Samples. Four human FFPE liver samples were purchased from Folio Biosciences. Two mouse FFPE liver samples and two mouse formalinFull Paper
2015Rokyta, DR;Wray, KP;McGivern, JJ;Margres, MJ;The transcriptomic and proteomic basis for the evolution of a novel venom phenotype within the Timber Rattlesnake (Crotalus horridus)Toxicon25727380Sequencing and nonnormalized cDNA library preparation for our two adults were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially aFull Paper
2015Pawar, H;Srikanth, SM;Kashyap, MK;Sathe, G;Chavan, S;Singal, M;Manju, HC;Kumar, KV;Vijayakumar, M;Sirdeshmukh, R;Pandey, A;Prasad, TS;Gowda, H;Kumar, RV;Downregulation of S100 Calcium Binding Protein A9 in Esophageal Squamous Cell CarcinomaScientificWorldJournal26788548The ESCC tumors varied from Grade I to Grade III and were obtained from patients in the age group of 36 to 78 years and (ii) FolioBio (Powell, OH; Catalog number ARY-HH0091) consisting of 40 ESCC cases with matched adjacent normal esophageal epitheliFull Paper
2015Krönke, J;Fink, EC;Hollenbach, PW;MacBeth, KJ;Hurst, SN;Udeshi, ND;Chamberlain, PP;Mani, DR;Man, HW;Gandhi, AK;Svinkina, T;Schneider, RK;McConkey, M;Järås, M;Griffiths, E;Wetzler, M;Bullinger, L;Cathers, BE;Carr, SA;Chopra, R;Ebert, BL;Lenalidomide induces ubiquitination and degradation of CK1α in del(5q) MDSNature26131937uced cells in each condition was assessed after 5 days by flow cytometry. Control cord-blood CD34+ cells were isolated as above. Adult bone marrow CD34+ cells were purchased as single-donor lots from AllCells (Alameda, CA). The number of replicates fFull Paper
2015Hendel, A;Bak, RO;Clark, JT;Kennedy, AB;Ryan, DE;Roy, S;Steinfeld, I;Lunstad, BD;Kaiser, RJ;Wilkens, AB;Bacchetta, R;Tsalenko, A;Dellinger, D;Bruhn, L;Porteus, MH;Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cellsNature biotechnology26121415Cas9-encoding plasmid. Mobilized human peripheral blood CD34+ HSPCs were purchased from AllCells and thawed according to manufacturer’s instructions. CD34+ HSPCs were maintained in X-VIVO 15 (Lonza) supplemented with SCF (100 ng/ml), TPO (100 ng/ml),Full Paper
2015Kotini, AG;Chang, CJ;Boussaad, I;Delrow, JJ;Dolezal, EK;Nagulapally, AB;Perna, F;Fishbein, GA;Klimek, VM;Hawkins, RD;Huangfu, D;Murry, CE;Graubert, T;Nimer, SD;Papapetrou, EP;Functional analysis of a chromosomal deletion associated with myelodysplastic syndromes using isogenic human induced pluripotent stem cellsNature biotechnology25798938CB CD34 + cells were purchased from AllCells. Two to four shRNAs against each of the four genes HIPK2, ATP6V0E2, LUC7L2 and EZH2 and one scramble sequence were inserted in SaveFull Paper
2015Levine, JH;Simonds, EF;Bendall, SC;Davis, KL;Amir, el-AD;Tadmor, MD;Litvin, O;Fienberg, HG;Jager, A;Zunder, ER;Finck, R;Gedman, AL;Radtke, I;Downing, JR;Pe'er, D;Nolan, GP;Data-Driven Phenotypic Dissection of AML Reveals Progenitor-like Cells that Correlate with PrognosisCell26095251For healthy adult controls, cryopreserved healthy BMMCs were purchased from AllCells (Emeryville, CA). All human samples were obtained with informed consent in compliance with SaveFull Paper
2015Moraga, I;Wernig, G;Wilmes, S;Gryshkova, V;Richter, CP;Hong, WJ;Sinha, R;Guo, F;Fabionar, H;Wehrman, TS;Krutzik, P;Demharter, S;Plo, I;Weissman, IL;Minary, P;Majeti, R;Constantinescu, SN;Piehler, J;Garcia, KC;Tuning cytokine receptor signaling by re-orienting dimer geometry with surrogate ligandsCell25728669ficolled cells (32K) from a single healthy donor (donor ID #: 6024, lot#: BM4871) were purchased from Allcells, Alameda, CA 94502. Cells were subsequently treated with DNAase. Milteny CD34 magnetic beads were used to enrich for CD34+ cells withFull Paper
2015Vierstra, J;Reik, A;Chang, KH;Stehling-Sun, S;Zhou, Y;Hinkley, SJ;Paschon, DE;Zhang, L;Psatha, N;Bendana, YR;O'Neil, CM;Song, AH;Mich, AK;Liu, PQ;Lee, G;Bauer, DE;Holmes, MC;Orkin, SH;Papayannopoulou, T;Stamatoyannopoulos, G;Rebar, EJ;Gregory, PD;Urnov, FD;Stamatoyannopoulos, JA;Functional footprinting of regulatory DNANature methods26322838Human mobilized CD34 + cells (adult) were purchased from AllCells. Small-scale mRNA transfections were performed with a BTX device (Harvard Apparatus), using the CD34 + cell SaveFull Paper
2015Bunin, A;Sisirak, V;Ghosh, HS;Grajkowska, LT;Hou, ZE;Miron, M;Yang, C;Ceribelli, M;Uetani, N;Chaperot, L;Plumas, J;Hendriks, W;Tremblay, ML;Häcker, H;Staudt, LM;Green, PH;Bhagat, G;Reizis, B;Protein Tyrosine Phosphatase PTPRS Is an Inhibitory Receptor on Human and Murine Plasmacytoid Dendritic CellsImmunity26231120PBMC were isolated from healthy adult volunteers by Histopaque density gradient centrifugation; where indicated, pDCs were enriched using the Diamond pDC isolation kit (Miltenyi Biotec) to 95% purity. Human PBMC subsets for expression analysis were pFull Paper
2015Behbehani, GK;Samusik, N;Bjornson, ZB;Fantl, WJ;Medeiros, BC;Nolan, GP;Mass Cytometric Functional Profiling of Acute Myeloid Leukemia Defines Cell-Cycle and Immunophenotypic Properties That Correlate with Known Responses to TherapyCancer discovery26091827Healthy control samples were obtained from Allcells using the same protocol. The exception was healthy donor sample #1; this sample was incubated for approximately 1 hour with IdU SaveFull Paper
2015Casero, D;Sandoval, S;Seet, CS;Scholes, J;Zhu, Y;Ha, VL;Luong, A;Parekh, C;Crooks, GM;Long non-coding RNA profiling of human lymphoid progenitor cells reveals transcriptional divergence of B cell and T cell lineagesNature immunology26502406Anonymous normal human thymi were obtained via the UCLA Translational Pathology Core Laboratory and the CHLA cardiovascular thoracic surgery department. Bone marrow (BM) was obtained from donors via ALLCELLS, Alameda, CA. All tissues were collected aFull Paper
2015Swaminathan, S;Klemm, L;Park, E;Papaemmanuil, E;Ford, A;Kweon, SM;Trageser, D;Hasselfeld, B;Henke, N;Mooster, J;Geng, H;Schwarz, K;Kogan, SC;Casellas, R;Schatz, DG;Lieber, MR;Greaves, MF;Müschen, M;Mechanisms of clonal evolution in childhood acute lymphoblastic leukemiaNature immunology25985233Human CD19 + cord blood B cells were obtained from AllCells. AllCells procures cord blood subsequent to receiving informed consent from its donors. We have approval to use cord SaveFull Paper
2015Pandolfi, A;Stanley, RF;Yu, Y;Bartholdy, B;Pendurti, G;Gritsman, K;Boultwood, J;Chernoff, J;Verma, A;Steidl, U;PAK1 is a therapeutic target in acute myeloid leukemia and myelodysplastic syndromeBlood26170031Poor clinical outcome of acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS) has been attributed to failure of current chemotherapeutic regimens to target leukemic SaveFull Paper
2015Patel, V;Balakrishnan, K;Keating, MJ;Wierda, WG;Gandhi, V;Expression of executioner procaspases and their activation by a procaspase-activating compound in chronic lymphocytic leukemia cellsBlood25538042Normal B lymphocytes (freshly isolated negatively selected CD19 + B cells) were purchased from AllCells, and were kept in RPMI-1640 medium with 10% fetal bovine serum (FBS). SaveFull Paper
2015Iwasa, M;Miura, Y;Fujishiro, A;Fujii, S;Sugino, N;Yoshioka, S;Tamura, A;Sato, A;Yokota, A;Kito, K;Ando, A;Hirai, H;Takaori-Kondo, A;Ichinohe, T;Maekawa, T;Bortezomib Attenuates Adhesion of B Cell Precursor Acute Lymphoblastic Lleukemia Cells to Bone Marrow Mesenchymal Stromal/Stem Cells Via Regulating SPARC ExpressionBloodWe isolated human BM-MSCs from normal bone marrow samples (AllCells, Emeryville, CA). When Nalm6 cells were co-cultured with human BM-MSCs, they were prone to adhere to BM Save;The prognosis of adults with B cell precursor acute lymphoblastic leukemFull Paper
2015Shenoy, S;Eapen, M;Wu, J;Walters, M;Levine, J;Logan, B;Gersten, I;Kamani, N;A Multicenter Phase II Trial of Unrelated Donor Reduced Intensity Bone Marrow Transplantation for Children with Severe Sickle Cell Disease (SCURT): Results of the Blood and Marrow Transplant Clinical Trials Network (BMT CTN 0601) StudyBloodBackground: Children with severe sickle cell disease (SCD) experience organ damage, poor quality of life, and a risk of premature mortality. Allogeneic bone marrow transplantation (BMT) halts disease progression when stable donor erythFull Paper
2015Krishnamurti, L;Sullivan, K;Kamani, N;Waller, E;Abraham, A;Campigotto, F;Zhang, W;Smith, S;Hassell, K;Decastro, L;Wu, C;Neuberg, D;Walters, M;Results of a Multicenter Pilot Investigation of Bone Marrow Transplantation in Adults with Sickle Cell Disease (STRIDE)BloodBackground: Hematopoietic cell transplantation (HCT) for sickle cell disease (SCD) is potentially curative, yet applied sparingly in adults due in part to limited donor availability and to concerns about safety. We conducted a multicenter pilot cliniFull Paper
2015Cappelli, B;Bernaudin, F;Ruggeri, A;Labopin, M;Volt, F;Simoes, B;Ferster, A;Dupont, S;de la Fuente, J;Dalle, J;Zecca, M;Robin, M;Walters, M;Dhedin, N;Michel, G;Lutz, P;Neven, B;Bertrand, Y;Vannier, J;Ayas, M;Matthes, S;Elayoubi, H;Devergie, A;Kenzey, C;Locatelli, F;Peters, C;Rocha, V;Eapen, M;Gluckman, E;Hematopoietic Stem Cell Transplantation from HLA Identical Sibling Forsickle Cell Disease an International Survey on Behalf of Eurocord-Monacord, EBMT Paediatric Disease Working Party and CIBMTRBloodIntroduction: Hematopoietic stem cell transplantation (HSCT) is, to date, the only curative therapy for sickle cell disease (SCD). However, HSCT is offered to relatively few patients with SCD for a number of reasons including lack of aFull Paper
2015Kanter, J;Walters, M;Hsieh, M;Thompson, A;Krishnamurti, L;Kwiatkowski, J;Kamble, R;von Kalle, C;Kuypers, F;Cavazzana, M;Leboulch, P;Sandler, L;Soni, S;Tisdale, J;Initial Results from Study Hgb-206: A Phase 1 Study Evaluating Gene Therapy By Transplantation of Autologous CD34+ Stem Cells Transduced Ex Vivo with the Lentiglobin BB305 Lentiviral Vector in Subjects with Severe Sickle Cell DiseaseBloodBackground: Hematopoietic stem cell (HSC) gene transfer has the potential to reduce or eliminate the symptoms of severe sickle cell disease (SCD). Previously reported early results in a single subject with severe SCD from the ongoing study HGB-205 suFull Paper
2015Walters, M;Rasko, J;Hongeng, S;Kwiatkowski, J;Schiller, G;Kletzel, M;Ho, P;Vichinsky, E;von Kalle, C;Cavazzana, M;Leboulch, P;Petrusich, A;Soni, S;Thompson, A;Update of Results from the Northstar Study (HGB-204): A Phase 1/2 Study of Gene Therapy for Beta-Thalassemia Major Via Transplantation of Autologous Hematopoietic Stem Cells Transduced Ex-Vivo with a Lentiviral Beta AT87Q-Globin Vector (LentiGlobin BB305 Drug Product)BloodBackground: Hematopoietic stem cell (HSC) gene transfer has the potential to induce globin production and mitigate or eliminate blood transfusions in patients with β-thalassemia major. Previously reported early results in subjects with β-thalassemiaFull Paper
2015Fujishiro, A;Miura, Y;Iwasa, M;Fujii, S;Tamura, A;Sato, A;Yokota, A;Sugino, N;Hirai, H;Ando, A;Ichinohe, T;Maekawa, T;Vitamin K2 Supports Hematopoiesis through Acting on Bone Marrow Mesenchymal Stromal/Stem CellsBlood[Methods] Normal bone marrow (BM) samples from healthy adult volunteers were purchased from AllCells (Emeryville, CA). BM-CD34 + cells were isolated from BM-mononuclear cells Save;[Background] Myelodysplastic syndrome is an intractable disorder chaFull Paper
2015Kikushige, Y;Miyamoto, T;Yuda, J;Jabbarzadeh-Tabrizi, S;Shima, T;Takayanagi, S;Niiro, H;Yurino, A;Miyawaki, K;Takenaka, K;Iwasaki, H;Akashi, K;A TIM-3/Gal-9 Autocrine Stimulatory Loop Drives Self-Renewal of Human Myeloid Leukemia Stem Cells and Leukemic ProgressionCell stem cell26279267summarizes the primary AML patients’ characteristics analyzed in this study. Human adult BM and PB samples were obtained from healthy donors or purchased from AllCells. CB cells were obtained from full-term deliveries (provided by the Kyushu Block ReFull Paper
2015Jeong, JY;Levine, MS;Abayasekara, N;Berliner, N;Laubach, J;Vanasse, GJ;The non-peptide thrombopoietin receptor agonist eltrombopag stimulates megakaryopoiesis in bone marrow cells from patients with relapsed multiple myelomaJournal of hematology & oncology25886818were also obtained from commercial sources (AllCells, Emeryville, CA and Proteogenix, from Lonza (Basel, Switzerland) and AllCells, respectively. Normal human platelets were SaveFull Paper
2015Goldstein, RL;Yang, SN;Taldone, T;Chang, B;Gerecitano, J;Elenitoba-Johnson, K;Shaknovich, R;Tam, W;Leonard, JP;Chiosis, G;Cerchietti, L;Melnick, A;Pharmacoproteomics identifies combinatorial therapy targets for diffuse large B cell lymphomaThe Journal of clinical investigation26529251HEPES, and penicillin/streptomycin. CD19+ cells were purchased from AllCells. PU-H71 was synthesized as previously reported (5). Ibrutinib was provided by Pharmacyclics. R406, BKM120, and sotrastaurin were purchased from Selleck Chemicals. MI-2 was pFull Paper
2015Ma, SD;Xu, X;Plowshay, J;Ranheim, EA;Burlingham, WJ;Jensen, JL;Asimakopoulos, F;Tang, W;Gulley, ML;Cesarman, E;Gumperz, JE;Kenney, SC;LMP1-deficient Epstein-Barr virus mutant requires T cells for lymphomagenesisThe Journal of clinical investigation25485679Alternatively, commercially purchased CD34-depleted human cord blood mononuclear cells (AllCells LLC, catalog no. CB117) were mock-infected or infected with WT EBV or LMP1-KO EBV in vitro for 1.5 hours, after which 12 × 106-25 × 106 cells were injectFull Paper
2015Chichili, GR;Huang, L;Li, H;Burke, S;He, L;Tang, Q;Jin, L;Gorlatov, S;Ciccarone, V;Chen, F;Koenig, S;Shannon, M;Alderson, R;Moore, PA;Johnson, S;Bonvini, E;A CD3xCD123 bispecific DART for redirecting host T cells to myelogenous leukemia: preclinical activity and safety in nonhuman primatesScience translational medicine26019218Cryopreserved AML patient primary PBMCs and bone marrow mononuclear cells (BMMNCs) were purchased from AllCells, LLC, which obtains biological specimens from a donor SaveFull Paper
2015Lee, ST;Muench, MO;Fomin, ME;Xiao, J;Zhou, M;de Smith, A;Martín-Subero, JI;Heath, S;Houseman, EA;Roy, R;Wrensch, M;Wiencke, J;Metayer, C;Wiemels, JL;Epigenetic remodeling in B-cell acute lymphoblastic leukemia occurs in two tracks and employs embryonic stem cell-like signaturesNucleic acids research25690899l, pre-B-II cells for whole genome bisulfite sequencing (WGBS) were purified. Purified normal mature B-cells, T-cells, regulatory T-cells, NK-cells, monocytes and granulocytes were purchased from the Allcells Corp. DNAs were extracted as described abFull Paper
2015Jung, N;Dai, B;Gentles, AJ;Majeti, R;Feinberg, AP;An LSC epigenetic signature is largely mutation independent and implicates the HOXA cluster in AML pathogenesisNature communications26444494n cancer biology using large data sets. Methods Human samples Fresh human bone marrow mononuclear cells (BMMCs) from healthy donors (2 × 108 cells per donor, Catalogue#: ABM006) were purchased from ALLCELLS (Emeryville, CA). A CD34+ cell-enrichmentFull Paper
2015Naka, K;Jomen, Y;Ishihara, K;Kim, J;Ishimoto, T;Bae, EJ;Mohney, RP;Stirdivant, SM;Oshima, H;Oshima, M;Kim, DW;Nakauchi, H;Takihara, Y;Kato, Y;Ooshima, A;Kim, SJ;Dipeptide species regulate p38MAPK-Smad3 signalling to maintain chronic myelogenous leukaemia stem cellsNature communications26289811Viable BM MNCs from three human patients with chronic phase CML were purchased from Allcells (#06-255, #06-620 and #147742, Alameda, CA, USA). We stained these cells with SaveFull Paper
2015Buchner, M;Park, E;Geng, H;Klemm, L;Flach, J;Passegué, E;Schjerven, H;Melnick, A;Paietta, E;Kopanja, D;Raychaudhuri, P;Müschen, M;Identification of FOXM1 as a therapeutic target in B-cell lineage acute lymphoblastic leukaemiaNature communications25753524nslocation of oestrogen receptor fusion proteins, 4-OHT was added at a concentration of 500 nM. Cell sorting For magnetic bead sorting, peripheral blood or BM mononuclear cells were purchased from AllCells LLC and CD19-enrichment was performed by maFull Paper
2015Zhang, W;Wray, LS;Rnjak-Kovacina, J;Xu, L;Zou, D;Wang, S;Zhang, M;Dong, J;Li, G;Kaplan, DL;Jiang, X;Vascularization of hollow channel-modified porous silk scaffolds with endothelial cells for tissue regenerationBiomaterials25934280HUVECs were purchased from AllCells biology technology limited company (Shanghai, China). HUVECs were cultured in endothelial basal medium (EBM, AllCells) devoid of growth SaveFull Paper
2015Coquery, CM;Loo, WM;Wade, NS;Bederman, AG;Tung, KS;Lewis, JE;Hess, H;Erickson, LD;BAFF regulates follicular helper t cells and affects their accumulation and interferon-γ production in autoimmunityArthritis & rheumatology (Hoboken, N.J.)25385309Additional blood from SLE patients was purchased from AllCells to increase the range of SLE Disease Activity Index (SLEDAI) scores 24. AllCells used the same protocol and materials SaveFull Paper
2015Stephenson, ST;Brown, LA;Helms, MN;Qu, H;Brown, SD;Brown, MR;Fitzpatrick, AM;Cysteine oxidation impairs systemic glucocorticoid responsiveness in children with difficult-to-treat asthmaThe Journal of allergy and clinical immunology25748343Cell culture experiments were performed with THP-1 monocytes (ATCC®, Manassas, VA), MM.1S B lymphoblasts (ATCC®) and primary PBMCs from healthy donors (AllCells, Alameda, CA). Reduced and oxidized conditions were created by adding 300 μL of 10 mM cysFull Paper
2015Karjalainen, K;Jaalouk, DE;Bueso-Ramos, C;Bover, L;Sun, Y;Kuniyasu, A;Driessen, WH;Cardó-Vila, M;Rietz, C;Zurita, AJ;O'Brien, S;Kantarjian, HM;Cortes, JE;Calin, GA;Koivunen, E;Arap, W;Pasqualini, R;Targeting IL11 Receptor in Leukemia and Lymphoma: A Functional Ligand-Directed Study and Hematopathology Analysis of Patient-Derived SpecimensClinical cancer research : an official journal of the American Association for Cancer Research25779950Normal blood and bone marrow samples were commercially obtained (AllCells). Cells were maintained in humidified hypoxia chambers (HeraCell 150; Thermo Electron Corporation) SaveFull Paper
2015Morelli, E;Leone, E;Cantafio, ME;Di Martino, MT;Amodio, N;Biamonte, L;Gullà, A;Foresta, U;Pitari, MR;Botta, C;Rossi, M;Neri, A;Munshi, NC;Anderson, KC;Tagliaferri, P;Tassone, P;Selective targeting of IRF4 by synthetic microRNA-125b-5p mimics induces anti-multiple myeloma activity in vitro and in vivoLeukemia25987254Biosciences, CA, USA); lentiviral particles were produced and transduced as previously described.19 RNA extraction and qRT-PCR. RNA samples of healthy donors BM-derived plasma cells were purchased (AllCells LLC, Alameda, CA, USA). Total RNA extractiFull Paper
2015Holm, F;Hellqvist, E;Mason, CN;Ali, SA;Delos-Santos, N;Barrett, CL;Chun, HJ;Minden, MD;Moore, RA;Marra, MA;Runza, V;Frazer, KA;Sadarangani, A;Jamieson, CH;Reversion to an embryonic alternative splicing program enhances leukemia stem cell self-renewalProceedings of the National Academy of Sciences of the United States of America26621726from patients at the University of California, San Diego and the University of Toronto Health Network according to Institutional Review Board-approved informed consent protocols. Human CD34+ cord blood cells and NPB cells were purchased from AllCellsFull Paper
2015Sundaravel, S;Duggan, R;Bhagat, T;Ebenezer, DL;Liu, H;Yu, Y;Bartenstein, M;Unnikrishnan, M;Karmakar, S;Liu, TC;Torregroza, I;Quenon, T;Anastasi, J;McGraw, KL;Pellagatti, A;Boultwood, J;Yajnik, V;Artz, A;Le Beau, MM;Steidl, U;List, AF;Evans, T;Verma, A;Wickrema, A;Reduced DOCK4 expression leads to erythroid dysplasia in myelodysplastic syndromesProceedings of the National Academy of Sciences of the United States of America26578796CD34 + HSCs and progenitor cells were purified from G-mobilized peripheral blood of healthy donors purchased from AllCells, Inc. using a CliniMACS (Miltenyi Biotec, Inc.) instrument. SaveFull Paper
2015Yea, K;Zhang, H;Xie, J;Jones, TM;Lin, CW;Francesconi, W;Berton, F;Fallahi, M;Sauer, K;Lerner, RA;Agonist antibody that induces human malignant cells to kill one anotherProceedings of the National Academy of Sciences of the United States of America26487683The one patient with M5 differentiated cells had been treated with Ara-C for 4 mo before collection of cells; the other six patients were untreated. Samples of leukemic cells obtained by BM aspiration or from PB were purchased from the AllCells corpoFull Paper
2015Lovat, F;Fassan, M;Gasparini, P;Rizzotto, L;Cascione, L;Pizzi, M;Vicentini, C;Balatti, V;Palmieri, D;Costinean, S;Croce, CM;miR-15b/16-2 deletion promotes B-cell malignanciesProceedings of the National Academy of Sciences of the United States of America26324892Technologies) according to the manufacturer’s protocol. Real-time PCR experiments were carried out using miR-15b and pri-miR-15b assays for real-time PCR (Applied Biosystems) according to the manufacturer’s protocol. Control human cord blood CD19+ BFull Paper
2015Mori, Y;Chen, JY;Pluvinage, JV;Seita, J;Weissman, IL;Prospective isolation of human erythroid lineage-committed progenitorsProceedings of the National Academy of Sciences of the United States of America26195758Antibodies, Cell Staining, and Sorting. The sorting procedures for HSCs and myeloid progenitor populations that we previously reported (20) were slightly modified. In brief, bone marrow mononuclear cells, purchased from AllCells, were first stained wFull Paper
2015Li, Y;Rogoff, HA;Keates, S;Gao, Y;Murikipudi, S;Mikule, K;Leggett, D;Li, W;Pardee, AB;Li, CJ;Suppression of cancer relapse and metastasis by inhibiting cancer stemnessProceedings of the National Academy of Sciences of the United States of America25605917Human CD34 + bone marrow cells (AllCells) were cultured in MethoCult media to form colonies as described by the manufacturer (Stemcell Technologies). BBI608 (2-Acetyl-4H,9H- SaveFull Paper
2015Kuramitsu, M;Okuma, K;Yamochi, T;Sato, T;Sasaki, D;Hasegawa, H;Umeki, K;Kubota, R;Sobata, R;Matsumoto, C;Kaneko, N;Naruse, I;Yamagishi, M;Nakashima, M;Momose, H;Araki, K;Mizukami, T;Mizusawa, S;Okada, Y;Ochiai, M;Utsunomiya, A;Koh, KR;Ogata, M;Nosaka, K;Uchimaru, K;Iwanaga, M;Sagara, Y;Yamano, Y;Satake, M;Okayama, A;Mochizuki, M;Izumo, S;Saito, S;Itabashi, K;Kamihira, S;Yamaguchi, K;Watanabe, T;Hamaguchi, I;Standardization of Quantitative PCR for Human T-Cell Leukemia Virus Type 1 in Japan: a Collaborative StudyJournal of clinical microbiology26292315PBMCs were purchased from AllCells Inc. (Alameda, CA, USA). TL-Om1 cells were in RPMI 1640 supplemented with 10% FBS at 37C according to the protocol provided by AllCells Inc. SaveFull Paper
2015Kuramitsu, M;Okuma, K;Yamagishi, M;Yamochi, T;Firouzi, S;Momose, H;Mizukami, T;Takizawa, K;Araki, K;Sugamura, K;Yamaguchi, K;Watanabe, T;Hamaguchi, I;Identification of TL-Om1, an adult T-cell leukemia (ATL) cell line, as reference material for quantitative PCR for human T-lymphotropic virus 1Journal of clinical microbiology25502533PBMCs were kindly provided by the Japanese Red Cross or purchased from AllCells (Alameda, CA, USA). TL-Om1 cells, an ATL-derived cell line established by Sugamura et al. (17), were maintained in RPMI 1640 (Sigma, St. Louis, MO, USA) containing 10% feFull Paper
2015Lancet, JE;Roboz, GJ;Cripe, LD;Michelson, GC;Fox, JA;Leavitt, RD;Chen, T;Hawtin, R;Craig, AR;Ravandi, F;Maris, MB;Stuart, RK;Karp, JE;A phase 1b/2 study of vosaroxin in combination with cytarabine in patients with relapsed or refractory acute myeloid leukemiaHaematologica25381131clinical samples, induction of pDNA-PKcs following a 2 h exposure to vosaroxin was examined. PBMC from an AML patient (80% blasts; obtained from AllCells LLC) were treated ex vivo with a dose titration of vosaroxin or vehicle for 2 h. Samples were anFull Paper
2015Sanyal, M;Morimoto, M;Baradaran-Heravi, A;Choi, K;Kambham, N;Jensen, K;Dutt, S;Dionis-Petersen, KY;Liu, LX;Felix, K;Mayfield, C;Dekel, B;Bokenkamp, A;Fryssira, H;Guillen-Navarro, E;Lama, G;Brugnara, M;Lücke, T;Olney, AH;Hunley, TE;Polat, AI;Yis, U;Bogdanovic, R;Mitrovic, K;Berry, S;Najera, L;Najafian, B;Gentile, M;Nur Semerci, C;Tsimaratos, M;Lewis, DB;Boerkoel, CF;Lack of IL7Rα expression in T cells is a hallmark of T-cell immunodeficiency in Schimke immuno-osseous dysplasia (SIOD)Clinical immunology (Orlando, Fla.)26499378The protocol and informed consent procedures were provided according to the Declaration of Helsinki guidelines. The clinical data for patients were obtained from questionnaires completed by the attending physician as well as from the medical records.Full Paper
2015Liu, J;Gaj, T;Wallen, MC;Barbas, CF;Improved cell-penetrating zinc-finger nuclease proteins for precision genome engineeringMolecular therapy. Nucleic acids25756962T-activator Dynabeads (Dynal/Life Technologies), and 50 U/ml recombinant interleukin-2 (rIL-2; R&D Systems). Bone marrow-derived CD34+ hematopoietic stem/progenitor cells (HSPCs) were obtained from AllCells, LLC at a purity of >97% (Supplementary FigFull Paper
2015Kim, SY;Theunissen, JW;Balibalos, J;Liao-Chan, S;Babcock, MC;Wong, T;Cairns, B;Gonzalez, D;van der Horst, EH;Perez, M;Levashova, Z;Chinn, L;D'Alessio, JA;Flory, M;Bermudez, A;Jackson, DY;Ha, E;Monteon, J;Bruhns, MF;Chen, G;Migone, TS;A novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignanciesBlood cancer journal26024286Additional frozen AML and CLL patient specimens for flow analysis were from AllCells and the University of California San Diego, respectively. Primary solid tumors and normal SaveFull Paper
2015Crews, LA;Jiang, Q;Zipeto, MA;Lazzari, E;Court, AC;Ali, S;Barrett, CL;Frazer, KA;Jamieson, CH;An RNA editing fingerprint of cancer stem cell reprogrammingJournal of translational medicine25889244Peripheral blood or bone marrow samples were processed by Ficoll density centrifugation and viable cells stored in liquid nitrogen. Normal peripheral blood mononuclear cells (MNC) were obtained from AllCells (Alameda, CA). Mononuclear cells from contFull Paper
2015Sloan, DD;Lam, CY;Irrinki, A;Liu, L;Tsai, A;Pace, CS;Kaur, J;Murry, JP;Balakrishnan, M;Moore, PA;Johnson, S;Nordstrom, JL;Cihlar, T;Koenig, S;Targeting HIV Reservoir in Infected CD4 T Cells by Dual-Affinity Re-targeting Molecules (DARTs) that Bind HIV Envelope and Recruit Cytotoxic T CellsPLoS pathogens26539983pended with FACS buffer, and analyzed by flow cytometry using a FACSCalibur (BD Biosciences) and FlowJo software (TreeStar). In Vitro HIV-Infected Cell Killing Assay PBMCs from healthy participants (AllCells) were isolated by Ficoll-plaque gradient.Full Paper
2015Ma, L;Aijima, R;Hoshino, Y;Yamaza, H;Tomoda, E;Tanaka, Y;Sonoda, S;Song, G;Zhao, W;Nonaka, K;Shi, S;Yamaza, T;Transplantation of mesenchymal stem cells ameliorates secondary osteoporosis through interleukin-17-impaired functions of recipient bone marrow mesenchymal stem cells in MRL/lpr miceStem cell research & therapy26012584University (protocol number: A21-044-1). Human MSC isolation and culture Human MSCs were isolated based on the adherent colony-forming unit fibroblasts (CFU-F) method [20]. Bone marrow cells (BMCs) (AllCells, Berkeley, CA, USA) were seeded at 1×107Full Paper
2015Di Lullo, G;Marcatti, M;Heltai, S;Brunetto, E;Tresoldi, C;Bondanza, A;Bonini, C;Ponzoni, M;Tonon, G;Ciceri, F;Bordignon, C;Protti, MP;Th22 cells increase in poor prognosis multiple myeloma and promote tumor cell growth and survivalOncoimmunology26155400had approved the study protocol and written informed consent was obtained from all donors. Blood samples of 15 healthy donors from the Institutional Blood Bank and BM aspirates from 4 healthy donors (AllCells, Life Science Division) were used as contFull Paper
2015Alva, A;Friedlander, T;Clark, M;Huebner, T;Daignault, S;Hussain, M;Lee, C;Hafez, K;Hollenbeck, B;Weizer, A;Premasekharan, G;Tran, T;Fu, C;Ionescu-Zanetti, C;Schwartz, M;Fan, A;Paris, P;Circulating Tumor Cells as Potential Biomarkers in Bladder CancerThe Journal of urology25912492candidacy for cisplatin based chemotherapy and 4) candidacy for radical cystectomy with curative intent. Two patients were lost to followup. Of the patients 16 underwent pathological staging at cystectomy. A second cohort of 11 patients with metastasFull Paper
2015Yang, J;Khan, M;Zhang, L;Ren, X;Guo, J;Feng, Y;Wei, S;Zhang, W;Antimicrobial surfaces grafted random copolymers with REDV peptide beneficial for endothelializationJournal of materials chemistry. B32264578Human umbilical vein endothelial cells (HUVECs) were purchased from Allcells Biomart (Shanghai) and cultured in high glucose DMEM supplemented with 10% FBS in an incubator ( SaveFull Paper
2015Fernandes, S;Chong, JJH;Paige, SL;Iwata, M;Torok-Storb, B;Keller, G;Reinecke, H;Murry, CE;Comparison of Human Embryonic Stem Cell-Derived Cardiomyocytes, Cardiovascular Progenitors, and Bone Marrow Mononuclear Cells for Cardiac RepairStem cell reports26607951vol/vol), supplemented with L-glutamine, ascorbic acid, transferrin, monothioglycerol, cyclosporine A (200 nM, Wako), and Y-27632 (10 μM). Fresh bone marrow mononuclear cells were purchased from AllCells LLC (ABM024). After overnight shipment, theyFull Paper
2015McIntosh, BE;Brown, ME;Duffin, BM;Maufort, JP;Vereide, DT;Slukvin, II;Thomson, JA;Nonirradiated NOD,B6.SCID Il2rγ-/- Kit(W41/W41) (NBSGW) mice support multilineage engraftment of human hematopoietic cellsStem cell reports25601207cedures for oligos and allelic determination. The University of Wisconsin Medical School’s Animal Care and Use Committee authorized all experiments. Human Cell Engraftment Frozen human CD34+ CBCs (AllCells) were thawed per manufacturer’s recommendatFull Paper
2015Tang, W;Song, H;Cai, W;Shen, X;Real Time Monitoring of Inhibition of Adipogenesis and Angiogenesis by (-)-Epigallocatechin-3-Gallate in 3T3-L1 Adipocytes and Human Umbilical Vein Endothelial CellsNutrients26516907TGTCTTTA-3′ and reverse, 5′-AGCCCTCCACCTCCCTGTAG-3′; β-actin forward, 5′-CCTCTATGCCAACACAGT-3′ and reverse, 5′-AGCCACCAATCCACACAG 3′. 2.8. Co-Culture of HUVEC and 3T3-L1 Cells HUVECs (ALLCELLS, Shanghai, China) were seeded (6000 cells/well) into E-pFull Paper
2015Griffin, DO;Goff, SP;HIV-1 Is Restricted prior to Integration of Viral DNA in Primary Cord-Derived Human CD34+ CellsJournal of virology25995256CD34 cells were isolated with magnetic beads from cord blood samples obtained from the Long Island Blood Service or obtained preisolated from AllCells (11, 12). CD34 cells were put SaveFull Paper
2015Wu, X;Sereno, AJ;Huang, F;Lewis, SM;Lieu, RL;Weldon, C;Torres, C;Fine, C;Batt, MA;Fitchett, JR;Glasebrook, AL;Kuhlman, B;Demarest, SJ;Fab-based bispecific antibody formats with robust biophysical properties and biological activitymAbs25774965Untreated, purified T cells from a human donor were purchased from ALLCELLS (Cat#PB009-1F). The cells were thawed and washed in 10% FBS (heat inactivated, Corning) plus 50 SaveFull Paper
2015Li, X;Tian, F;Wang, F;The effects of black soybean (Glycine max var.) on chronic cervical pain of sedentary office workers in a northern Chinese populationFood & function262954835.0 × 105 cells per ml was placed into each well of a 96-well plate (ALLCELLS, Shanghai, China). After 24 h, cells were incubated with 1 Ci per well of [3H]thymidine (67 Ci mmol−1) for five h, and then collected using a Brandell 96-sample harvester (Full Paper
2015Mata, MF;Lopes, JP;Ishikawa, M;Alaiti, MA;Cabral, JM;da Silva, CL;Costa, MA;Scaling up the ex vivo expansion of human circulating CD34(+)progenitor cells with upregulation of angiogenic and anti-inflammatory potentialCytotherapy26475753CD34 + cells isolated from mobilized peripheral blood of healthy donors (n = 5) were obtained from AllCells and were cultured for 7 days in 30-mL cell culture bags (VueLife FEP Bag, SaveFull Paper
2015Ma, Y;Reilly, S;Fischer, S;Henshaw, M;Pierce, J;Poudel, A;Reems, J;Human platelet lysate produced at industrial scale for use as fetal bovine serum replacement in cell manufacturing protocolsCytotherapyTissue Origin: 9 different donors of fresh human bone marrow (hBM) from the posterior iliac crest were obtained from Lonza or AllCells. 6 different donors of fresh human lipoaspirate SaveFull Paper
2015Fierro, FA;O'Neal, AJ;Beegle, JR;Chávez, MN;Peavy, TR;Isseroff, RR;Egaña, JT;Hypoxic pre-conditioning increases the infiltration of endothelial cells into scaffolds for dermal regeneration pre-seeded with mesenchymal stem cellsFrontiers in cell and developmental biology26579521r bone marrow samples of healthy human donors who gave informed consent to the research protocols (approved by the institutional review board of the Technical University of Dresden) or purchased from Allcells (Alameda, CA). Mononuclear cell (MNC) fraFull Paper
2015Haqqani, AA;Marek, SL;Kumar, J;Davenport, M;Wang, H;Tilton, JC;Central memory CD4+ T cells are preferential targets of double infection by HIV-1Virology journal26559763Normal donor samples were de-identified and obtained from leukapheresis from ALLCELLS, LLC. All donors were negative for HBV, HCV, and HIV. A 10 year-old male with the SaveFull Paper
2015Huang, H;Saravia, J;You, D;Shaw, AJ;Cormier, SA;Impaired gamma delta T cell-derived IL-17A and inflammasome activation during early respiratory syncytial virus infection in infantsImmunology and cell biology25267484tes 2 hr prior to RSV infection. Sample isolation purity and adoptive transfer efficiency were verified by flow cytometry. CBMC/PBMC RIG-I activation Purified CBMCs and PBMCs from individual donors (AllCells.com) were thawed and counted according toFull Paper
2015Lynch, PJ;Thompson, EE;McGinnis, K;Rovira Gonzalez, YI;Lo Surdo, J;Bauer, SR;Hursh, DA;Chromatin Changes at the PPAR-γ2 Promoter During Bone Marrow-Derived Multipotent Stromal Cell Culture Correlate With Loss of Gene Activation PotentialStem cells (Dayton, Ohio)25640287BM-MSCs from six donors were purchased from AllCells (Alameda, CA, http://www.allcells.com/) and Lonza (Allendale, NJ, http://www.lonza.com/) (Table 1). All BM-MSCs used here were previously expanded, characterized, and cryopreserved for experimentatFull Paper
2015Münchow, EA;Albuquerque, MT;Zero, B;Kamocki, K;Piva, E;Gregory, RL;Bottino, MC;Development and characterization of novel ZnO-loaded electrospun membranes for periodontal regenerationDental materials : official publication of the Academy of Dental Materials26116414The guidelines provided by the International Standards Organization/ISO [35] were followed in order to detect toxicity levels of the electrospun membranes [34]. Human dental pulp stem cells (hDPSCs, AllCells LLC., Alameda, CA, USA) obtained from permFull Paper
2015Babad, J;Mukherjee, G;Follenzi, A;Ali, R;Roep, BO;Shultz, LD;Santamaria, P;Yang, OO;Goldstein, H;Greiner, DL;DiLorenzo, TP;Generation of β cell-specific human cytotoxic T cells by lentiviral transduction and their survival in immunodeficient human leucocyte antigen-transgenic miceClinical and experimental immunology25302633Several β cell antigens recognized by T cells in the non-obese diabetic (NOD) mouse model of type 1 diabetes (T1D) are also T cell targets in the human disease. While numerous SaveFull Paper
2015Liu, A;Wang, Y;Ding, Y;Baez, I;Payne, KJ;Borghesi, L;Cutting Edge: Hematopoietic Stem Cell Expansion and Common Lymphoid Progenitor Depletion Require Hematopoietic-Derived, Cell-Autonomous TLR4 in a Model of Chronic EndotoxinJournal of immunology (Baltimore, Md. : 1950)26276875De-identified cord blood cells were obtained with approval from the University of Pittsburgh School of Medicine Institutional Review Board. Purified CD34+ cord blood cells were purchased from AllCells, or cord blood obtained from Loma Linda UniversitFull Paper
2015Katano, I;Takahashi, T;Ito, R;Kamisako, T;Mizusawa, T;Ka, Y;Ogura, T;Suemizu, H;Kawakami, Y;Ito, M;Predominant development of mature and functional human NK cells in a novel human IL-2-producing transgenic NOG mouseJournal of immunology (Baltimore, Md. : 1950)25712215Germany) in combination with biotin-conjugated anti-mouse CD45 mAb (BD Biosciences), all cells, with the exception of human CD56+ NK cells, were magnetically labeled and eliminated using a MACS LD column (Miltenyi Biotec). The purity of the isolatedFull Paper
2015Montezuma-Rusca, JM;Moir, S;Kardava, L;Buckner, CM;Louie, A;Kim, LJ;Santich, BH;Wang, W;Fankuchen, OR;Diaz, G;Daub, JR;Rosenzweig, SD;Chun, TW;Li, Y;Braylan, RC;Calvo, KR;Fauci, AS;Bone marrow plasma cells are a primary source of serum HIV-1-specific antibodies in chronically infected individualsJournal of immunology (Baltimore, Md. : 1950)25681347obtained from posterior iliac crests under conscious sedation and local anesthesia. In addition, cryopreserved bone marrow mononuclear cells (BMMCs) were obtained from commercially available sources (Allcells or Stemcell Technologies)Full Paper
2015Kang, HS;Choi, SH;Kim, BS;Choi, JY;Park, GB;Kwon, TG;Chun, SY;Advanced Properties of Urine Derived Stem Cells Compared to Adipose Tissue Derived Stem Cells in Terms of Cell Proliferation, Immune Modulation and Multi DifferentiationJournal of Korean medical science26713051or separated-(trans-well chamber, Corning, Corning, NY, USA) cultured with both stem cells; each well was composed with 1:100, 1:50, 1:25, or 1:12.5 stem cell to PBMC ratio. PBMCs were acquired from AllCells (Emeryville, CA, USA) and activated with 5Full Paper
2015Wu, W;Zhang, X;Lv, H;Liao, Y;Zhang, W;Cheng, H;Deng, Z;Shen, J;Yuan, Q;Zhang, Y;Shen, W;Identification of immediate early response protein 2 as a regulator of angiogenesis through the modulation of endothelial cell motility and adhesionInternational journal of molecular medicine26260137cells (HUVECs) were purchased from AllCells (Shanghai, China) and cultured in 0.5% gelatin-coated dishes in endothelial cell growth medium (EGM; AllCells) according to the SaveFull Paper
2015Kamocki, K;Nör, JE;Bottino, MC;Dental pulp stem cell responses to novel antibiotic-containing scaffolds for regenerative endodonticsInternational endodontic journal25425048fetal bovine serum (FBS, Hyclone, Logan, UT, USA) and 1% penicillin- streptomycin (Sigma-Aldrich) in a humidified incubator at 37°C, with 5% CO2, was used to culture hDPSC (catalogue number - DP003F, AllCells LLC, Alameda, CA, USA) obtained from permFull Paper
2015Press, T;Viale-Bouroncle, S;Felthaus, O;Gosau, M;Morsczeck, C;EGR1 supports the osteogenic differentiation of dental stem cellsInternational endodontic journal24749562Dental follicle cells (DFCs), also called dental follicle stem cells (DFSCs), were purchased from AllCells© (Alameda, CA, USA). All cells were grown in standard basal medium, Dulbecco′s modified Eagle′s medium (DMEM; Sigma-Aldrich, Munich, Germany) sFull Paper
2015Lu, Q;Pandya, M;Rufaihah, AJ;Rosa, V;Tong, HJ;Seliktar, D;Toh, WS;Modulation of Dental Pulp Stem Cell Odontogenesis in a Tunable PEG-Fibrinogen Hydrogel SystemStem cells international26124841used in this study were obtained commercially from AllCells, LLC. DPSCs were cultured in Dulbecco's modified eagle's medium (DMEM) (low glucose, Biowest, France) supplemented SaveFull Paper
2015Chen, Q;Ye, W;Jian Tan, W;Mei Yong, KS;Liu, M;Qi Tan, S;Loh, E;Te Chang, K;Chye Tan, T;Preiser, PR;Chen, J;Delineation of Natural Killer Cell Differentiation from Myeloid Progenitors in HumanScientific reports26456148ercoll. Contaminating red blood cells were lysed with ACK lysis buffer (Life Technologies). Isolation of CD56+ cells from MNCs was performed via cell sorting. Human adult BM MNCs were purchased from AllCells (Alameda, CA). To isolate MNCs from fetalFull Paper
2015Su, M;Hu, R;Jin, J;Yan, Y;Song, Y;Sullivan, R;Lai, L;Efficient in vitro generation of functional thymic epithelial progenitors from human embryonic stem cellsScientific reports26044259ed (2 Gy), transplanted under the kidney capsule with the solidified reaggregate of the hESC-derived cells, and injected i.v. with human bone marrow CD34+ cells (1 × 105 cells/mouse, obtained from AllCells, LLC, Alameda, CA). Twelve to 24 weeks afterFull Paper
2015Nixon, CC;Schwartz, BH;Dixit, D;Zack, JA;Vatakis, DN;Cocaine exposure impairs multilineage hematopoiesis of human hematopoietic progenitor cells mediated by the sigma-1 receptor [corrected]Scientific reports25728014Mononuclear cells were obtained from the buffy coats of either processed fetal liver or cord blood or adult bone marrow (Allcells, Alameda, CA) following Ficoll-Hypaqe density gradient SaveFull Paper
2015Feitosa, SA;Münchow, EA;Al-Zain, AO;Kamocki, K;Platt, JA;Bottino, MC;Synthesis and characterization of novel halloysite-incorporated adhesive resinsJournal of dentistry26334950Next, human dental pulp stem cells (CD73, CD90, CD105, and CD166 positive) obtained from permanent third molars (hDPSCs, catalog number—DP003F, AllCells LLC, Alameda, CA, USA) [32] were cultured in DMEM supplemented with 10% FBS were seeded at the ceFull Paper
2015Lei, L;Wang, S;Wu, H;Ju, W;Peng, J;Qahtan, AS;Chen, C;Lu, Y;Peng, J;Zhang, X;Nie, H;Optimization of release pattern of FGF-2 and BMP-2 for osteogenic differentiation of low-population density hMSCsJournal of biomedical materials research. Part A24639043Fresh whole bone marrow was acquired from AllCells (Emeryville, CA). 0.5 mL of RosetteSep The hMSCs were isolated according to AllCells' protocol. Cells from passages 4-9 were SaveFull Paper
2015Poleganov, MA;Eminli, S;Beissert, T;Herz, S;Moon, JI;Goldmann, J;Beyer, A;Heck, R;Burkhart, I;Barea Roldan, D;Türeci, Ö;Yi, K;Hamilton, B;Sahin, U;Efficient Reprogramming of Human Fibroblasts and Blood-Derived Endothelial Progenitor Cells Using Nonmodified RNA for Reprogramming and Immune EvasionHuman gene therapy26381596Cord blood EPCs were derived from 5E7 frozen cord blood MNCs (AllCells). Both peripheral and cord blood MNCs were washed and cultured on rat collagen type I (BD Bioscience)- SaveFull Paper
2015Suenaga, H;Furukawa, KS;Suzuki, Y;Takato, T;Ushida, T;Bone regeneration in calvarial defects in a rat model by implantation of human bone marrow-derived mesenchymal stromal cell spheroidsJournal of materials science. Materials in medicine26449444p). After a recovery period of 8 weeks, bone regeneration at the defect sites was evaluated Isolation and culturing of human MSCs Fresh BM samples of 3-4 anonymous adult donors were obtained from AllCells (Berkeley, CA), and MSCs were isolated, as dFull Paper
2015Uchiyama, T;Kawabata, H;Miura, Y;Yoshioka, S;Iwasa, M;Yao, H;Sakamoto, S;Fujimoto, M;Haga, H;Kadowaki, N;Maekawa, T;Takaori-Kondo, A;The role of growth differentiation factor 15 in the pathogenesis of primary myelofibrosisCancer medicine26276681Normal human BM mononuclear cells were separated from BM aspirates (AllCells) using Ficoll-Hypaque-Plus solution (GE Healthcare, Tokyo, Japan). CD34 + HPCs (Hematopoietic SaveFull Paper
2015Wu, H;Fan, F;Liu, Z;Zhang, F;Liu, Y;Wei, Z;Shen, C;Cao, Y;Wang, A;Lu, Y;The angiogenic responses induced by release of angiogenic proteins from tumor cell-activated platelets are regulated by distinct molecular pathwaysIUBMB life26283102Gibco, Grand Island, NY), 100 U/mL penicillin and 100 μg/mL streptomycin, and grown in a 95% air and 5% CO2 humidified atmosphere at 37°C in Primary HUVECs were purchased from Allcells (Shanghai, China) and cultured in EBM media (Allcells) accordingFull Paper
2015Bam, R;Khan, S;Ling, W;Randal, SS;Li, X;Barlogie, B;Edmondson, R;Yaccoby, S;Primary myeloma interaction and growth in coculture with healthy donor hematopoietic bone marrowBMC cancer26545722itute. NBM coculture system Preparation of the NBM system is illustrated in Fig. 1a. Freshly obtained (via overnight shipping) whole BM cells from healthy adult (>40 years old) donors (n = 20, Allcells, Alameda, CA) were lysed with RBC lysis bufferFull Paper
2015Liao, W;Jordaan, G;Nham, P;Phan, RT;Pelegrini, M;Sharma, S;Gene expression and splicing alterations analyzed by high throughput RNA sequencing of chronic lymphocytic leukemia specimensBMC cancer26474785In all the CLL specimens, more than 90 % of isolated cells were CD19+ by flow cytometry analysis. Total RNA from isolated B cells (five different normal donors, caucasian males) was purchased from ALLCELLS (Alameda, CA). IGVH mutation (ImmunoglobulinFull Paper
2015Liu, L;Yu, H;Huang, X;Tan, H;Li, S;Luo, Y;Zhang, L;Jiang, S;Jia, H;Xiong, Y;Zhang, R;Huang, Y;Chu, CC;Tian, W;A novel engineered VEGF blocker with an excellent pharmacokinetic profile and robust anti-tumor activityBMC cancer25881012) in complete HUVEC-adapted medium (Cat#H-004, ALLCELLS) were incubated in 6 cm dishes at 37C, 5% CO 2 for 24 hours, cells were starved for 2 hours and then challenged for 15 SaveFull Paper
2015Priestman, MA;Wang, Q;Jernigan, FE;Chowdhury, R;Schmidt, M;Lawrence, DS;Multicolor monitoring of the proteasome's catalytic signatureACS chemical biology25347733m. All Proteasome inhibitors were purchased from Enzo Life Sciences. HeLa cells were purchased from the tissue culture facility at the University of North Carolina. Normal B-cells were purchased from AllCells. OCI-Ly3 and HBL-1 cells were provided byFull Paper
2015Yi, Y;Qi, H;Yuan, J;Wang, R;Weng, S;He, J;Dong, C;Functional characterization of viral tumor necrosis factor receptors encoded by cyprinid herpesvirus 3 (CyHV3) genomeFish & shellfish immunology26052019Primary human umbilical vein endothelial cells (HUVECs) were cultured in appropriate media according to the manufacturer's protocol (AllCells, China). The full length of CyHV3 ORF4 SaveFull Paper
2015Doss, JF;Corcoran, DL;Jima, DD;Telen, MJ;Dave, SS;Chi, JT;A comprehensive joint analysis of the long and short RNA transcriptomes of human erythrocytesBMC genomics26573221les as the input list to test for the enrichment of top RBC expressing transcripts against those more specific to D8 samples or the PBMC samples. Cell culture Mobilized Peripheral Blood CD34+ Cells (AllCells) were grown in StemLine II media containiFull Paper
2015Shi, J;Zhou, J;Zhang, M;Microcystins induces vascular inflammation in human umbilical vein endothelial cells via activation of NF-BMediators of InflammationPrimary HUVECs were obtained from Allcells (Shanghai, China) and cells in passages 3-5 were used in this study. Cells were grown in an RPMI 1640 medium containing 20% fetal SaveFull Paper
2015Zhang, Z;Luo, X;Xu, H;Wang, L;Jin, X;Chen, R;Ren, X;Lu, Y;Fu, M;Huang, Y;He, J;Fan, Z;Bone marrow stromal cell-derived extracellular matrix promotes osteogenesis of adipose-derived stem cellsCell biology international25264269BM-MSCs were purchased from ALLCELLS (Emeryville, CA) and expanded in culture medium [α-Minimal Essential Medium (Life Technologies, Grand Island, NY) supplemented with SaveFull Paper
2015Zhang, Q;Wang, LQ;Wong, KY;Li, ZY;Chim, CS;Infrequent DNA methylation of miR-9-1 and miR-9-3 in multiple myelomaJournal of clinical pathology25855800peripheral blood (PB) and three BM normal buffy coat), one CD138-sorted healthy marrow plasma cells (AllCells, California, USA), 10 cell lines (KMS-12-PE, LP-1, MOLP-8, NCI-H929, OPM-2, RPMI-8226, U-266, WL-2, OCI-MY5 and JJN-3), and primary MM marroFull Paper
2015Wang, DY;Gopinath, S;Lagacé, RE;Norona, W;Hennessy, LK;Short, ML;Mulero, JJ;Developmental validation of the GlobalFiler(®) Express PCR Amplification Kit: A 6-dye multiplex assay for the direct amplification of reference samplesForensic science international. Genetics26226223To demonstrate the sensitivity of the GlobalFiler Express Kit, white blood cells (WBCs) from two individuals were purchased from AllCells, LLC (Emeryville, CA) and quantitated using SaveFull Paper
2015Mahajan, S;Hogan, JK;Shlyakhter, D;Oh, L;Salituro, FG;Farmer, L;Hoock, TC;VX-509 (decernotinib) is a potent and selective janus kinase 3 inhibitor that attenuates inflammation in animal models of autoimmune diseaseThe Journal of pharmacology and experimental therapeutics25762693Sigma-Aldrich (St. Louis, MO); human B cells from AllCells (Emeryville, CA); Roswell Park Memorial Institute medium tissue culture medium, phosphate-buffered saline (PBS), and 10,000 U/ml penicillin-streptomycin from Life Technologies (Grand Island,Full Paper
2015Shabbir, A;Cox, A;Rodriguez-Menocal, L;Salgado, M;Van Badiavas, E;Mesenchymal Stem Cell Exosomes Induce Proliferation and Migration of Normal and Chronic Wound Fibroblasts, and Enhance Angiogenesis In VitroStem cells and development25867197Human MSCs were isolated and expanded from normal donor bone marrow acquired from AllCells LLC (Emeryville, CA, www.allcells.com). Three different donors were used for these experiments. MSCs were isolated by the plastic adherence method as describedFull Paper
2015Limb, JK;Song, D;Jeon, M;Han, SY;Han, G;Jhon, GJ;Bae, YS;Kim, J;2-(trimethylammonium)ethyl (R)-3-methoxy-3-oxo-2-stearamidopropyl phosphate promotes megakaryocytic differentiation of myeloid leukaemia cells and primary human CD34⁺ haematopoietic stem cellsJournal of tissue engineering and regenerative medicine23166016Human bone marrow-derived CD34+ cells were obtained from AllCells (Emeryville, CA, USA). Cells were plated at 8 × 104 cells/well in a 12-well plate and cultured for 16 days in Iscove's modified Dulbecco's medium (IMDM; Invitrogen) with 15% BIT 9500 (Full Paper
2015Leelakanok, N;Fischer, CL;Bates, AM;Guthmiller, JM;Johnson, GK;Salem, AK;Brogden, KA;Brogden, NK;Cytotoxicity of HBD3 for dendritic cells, normal human epidermal keratinocytes, hTERT keratinocytes, and primary oral gingival epithelial keratinocytes in cell culture conditionsToxicology letters26367466Human β-defensin 3 (HBD3) is a prominent host defense peptide. In our recent work, we observed that HBD3 modulates pro-inflammatory agonist-induced chemokine and cytokine SaveFull Paper
2015Gupta, MS;Nicoll, SB;Duration of TGF-β3 Exposure Impacts the Chondrogenic Maturation of Human MSCs in Photocrosslinked Carboxymethylcellulose HydrogelsAnnals of biomedical engineering25384834Human MSCs were purified from bone marrow (AllCells, Emeryville, CA) using enriched density centrifugation as previously described.15,28 These primary cells were then cultured using MSC growth medium containing Low Glucose Dulbecco’s Modified Eagle MFull Paper
2015Marino, MP;Panigaj, M;Ou, W;Manirarora, J;Wei, CH;Reiser, J;A scalable method to concentrate lentiviral vectors pseudotyped with measles virus glycoproteinsGene therapy25608718Human peripheral blood mononuclear cells were obtained from AllCells, LLC (Alameda, CA, USA). T cells were enriched by negative selection using the EasySep Human T Cell Enrichment Cocktail and EasySep D Magnetic Particles (Stem Cell Technologies, VanFull Paper
2015Shahaduzzaman, MD;Mehta, V;Golden, JE;Rowe, DD;Green, S;Tadinada, R;Foran, EA;Sanberg, PR;Pennypacker, KR;Willing, AE;Human umbilical cord blood cells induce neuroprotective change in gene expression profile in neurons after ischemia through activation of Akt pathwayCell transplantation25413246HUCB Cell Preparation Just prior to the OGD experiment, cryopreserved HUCB mononuclear cells (AllCells, LLC, Alameda, CA, USA) were thawed in a 37ºC water bath and then washed twice in 10 ml PBS (136.9 mM NaCl, 2.7 mM KCl, 10.1 mM Na2HPO4, 1.8 mM KH2Full Paper
2015Fukui, T;Mifune, Y;Matsumoto, T;Shoji, T;Kawakami, Y;Kawamoto, A;Ii, M;Akimaru, H;Kuroda, T;Horii, M;Yokoyama, A;Alev, C;Kuroda, R;Kurosaka, M;Asahara, T;Superior Potential of CD34-Positive Cells Compared to Total Mononuclear Cells for Healing of Nonunion Following Bone FractureCell transplantation24800622We purchased human GM-PB CD34+ cells obtained from three healthy males (20-year-old African-American; 19-year-old Caucasian; 21-year-old Caucasian) and human GM-PB MNCs obtained from three healthy males (25-year-old African-American; 25-year-old AfriFull Paper
2015Wada, A;Ito, A;Iitsuka, H;Tsuneyama, K;Miyazono, T;Murakami, J;Shibahara, N;Sakurai, H;Saiki, I;Nakayama, T;Yoshie, O;Koizumi, K;Sugiyama, T;Role of chemokine CX3CL1 in progression of multiple myeloma via CX3CR1 in bone microenvironmentsOncology reports25962684GT-3′ and antisense, 5′-CAT GTG GGC CAT GAG GTC CAC CAC-3′. The primers for CX3CR1 were as follows: sense, 5′-TGG CCT TGT CTG ATC TGC TGT TTG-3′ and antisense, 5′-ATG GCT TTG GCT TTC TTG TGG TTC-3′. The RNA from healthy donor plasma cell was purchaseFull Paper
2015Huang, Q;Zhou, C;Chen, X;Dong, B;Chen, S;Zhang, N;Liu, Y;Li, A;Yao, M;Miao, J;Li, Q;Wang, Z;Prodrug AST-003 Improves the Therapeutic Index of the Multi-Targeted Tyrosine Kinase Inhibitor SunitinibPloS one26513662Dr. Jiang Li at the Sun Yat-Sen University School of Medicine, which were purchased from Type Culture Collection of the Chinese Academy of Sciences in Shanghai, China. HUVEC cells were purchased from Allcells, Inc. The cell lines A549, Caki-1, HT-29,Full Paper
2015Lanman, RB;Mortimer, SA;Zill, OA;Sebisanovic, D;Lopez, R;Blau, S;Collisson, EA;Divers, SG;Hoon, DS;Kopetz, ES;Lee, J;Nikolinakos, PG;Baca, AM;Kermani, BG;Eltoukhy, H;Talasaz, A;Analytical and Clinical Validation of a Digital Sequencing Panel for Quantitative, Highly Accurate Evaluation of Cell-Free Circulating Tumor DNAPloS one26474073To assess the accuracy of our test, two micrograms of genomic DNA from 20 healthy and young donors (AllCells, Alameda, CA) were sent to an outside reference lab (Ambry Genetics, SaveFull Paper
2015Breese, EH;Buechele, C;Dawson, C;Cleary, ML;Porteus, MH;Use of Genome Engineering to Create Patient Specific MLL Translocations in Primary Human Hematopoietic Stem and Progenitor CellsPloS one26351841When fresh umbilical cord blood was not available, commercially available frozen human CD34+ cells isolated from umbilical cord blood were used (AllCells LLC or StemCell SaveFull Paper
2015Bose, C;Megyesi, JK;Shah, SV;Hiatt, KM;Hall, KA;Karaduta, O;Swaminathan, S;Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic FibrosisPloS one26305890ked immunosorbent assay (ELISA) by a commercially available kit (GenWay Biotech Inc., San Diego, CA) as instructed by the manufacturer. Cell culture and treatments Commercially available human PBMC (AllCells, Emeryville, CA) were used for in vitro sFull Paper
2015Ge, HY;Han, ZJ;Tian, P;Sun, WJ;Xue, DX;Bi, Y;Yang, ZH;Liu, P;VEGFA Expression Is Inhibited by Arsenic Trioxide in HUVECs through the Upregulation of Ets-2 and miRNA-126PloS one26274316, Shanghai, China) were cultured in complete medium (HUVEC-004, supplied by AllCells) in an atmosphere containing 5% CO 2 at 37C. All experiments were performed according to SaveFull Paper
2015Ninomiya, S;Kawano, M;Abe, T;Ishikawa, T;Takahashi, M;Tamura, M;Takahashi, Y;Nashimoto, M;Potential small guide RNAs for tRNase ZL from human plasma, peripheral blood mononuclear cells, and cultured cell linesPloS one25730316myeloma patients (MM1-MM3) were also obtained from AllCells. Written informed consent was obtained from each person by AllCells. In the case that human samples to be analyzed SaveFull Paper
2015Liu, Z;Benard, O;Syeda, MM;Schuster, VL;Chi, Y;Inhibition of Prostaglandin Transporter (PGT) Promotes Perfusion and Vascularization and Accelerates Wound Healing in Non-Diabetic and Diabetic RatsPloS one26230411lonal antibody (LifeSpan BioSciences, Seattle, WA, USA) interacting with rat tissues was used and the dilution was 1: 250. Cell Culture Fresh primary human bone marrow CD34+ cells were obtained from AllCells (Alameda, CA, USA) and cultured accordingFull Paper
2015Placencio, VR;Ichimura, A;Miyata, T;DeClerck, YA;Small Molecule Inhibitors of Plasminogen Activator Inhibitor-1 Elicit Anti-Tumorigenic and Anti-Angiogenic ActivityPloS one26207899HUVEC (human umbilical vein endothelial cells) were purchased from AllCells and maintained as recommended. HT1080 and HCT116 cells were cultured in DMEM medium SaveFull Paper
2015Fujii, H;Luo, ZJ;Kim, HJ;Newbigging, S;Gassas, A;Keating, A;Egeler, RM;Humanized Chronic Graft-versus-Host Disease in NOD-SCID il2rγ-/- (NSG) Mice with G-CSF-Mobilized Peripheral Blood Mononuclear Cells following Cyclophosphamide and Total Body IrradiationPloS one26176698G-CSF-mobilized human peripheral blood mononuclear cells (G-hPBMCs; 3 donors) and CD34 + cells from G-CSF-mobilized human PB were purchased from ALLCELLS (Emeryvill, CA SaveFull Paper
2015Yan, M;Wen, J;Liang, M;Lu, Y;Kamata, M;Chen, IS;Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAsPloS one26035832edium (Life Technologies, Carlsbad, CA) containing 10% FBS and supplemented with 2mM glutamine, 100 U/ml of penicillin, and 100 μg/ml of streptomycin. Mobilized human CD34+ hematopoietic stem cells (AllCells, Alameda, CA) were cultured in StepSpan SFFull Paper
2015Patterson, SD;Rossi, JM;Paweletz, KL;Fitzpatrick, VD;Begley, CG;Busse, L;Elliott, S;McCaffery, I;Functional EpoR pathway utilization is not detected in primary tumor cells isolated from human breast, non-small cell lung, colorectal, and ovarian tumor tissuesPloS one25807104were adjusted to 106 viable cells/mL prior to growth factor stimulation. For additional cell culture conditions, see S1 Appendix. Erythroid Progenitor-Cell (EPC) Assay Human CD34+ progenitor cells (AllCells Inc., Emeryville, CA) were isolated from bFull Paper
2015Yu, L;Croze, E;Yamaguchi, KD;Tran, T;Reder, AT;Litvak, V;Volkert, MR;Induction of a unique isoform of the NCOA7 oxidation resistance gene by interferon β-1bJournal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research25330068Peripheral blood mononuclear cells (PBMCs) derived from healthy individuals were obtained from AllCells. PBMCs (1×106cells/mL) and cultured cells were incubated at 37°C and 5.0% CO2 and grown in Eagle's MEM supplemented with 10% v/v heat-inactivatedFull Paper
2015Liang, X;Potter, J;Kumar, S;Zou, Y;Quintanilla, R;Sridharan, M;Carte, J;Chen, W;Roark, N;Ranganathan, S;Ravinder, N;Chesnut, JD;Rapid and highly efficient mammalian cell engineering via Cas9 protein transfectionJournal of biotechnology26003884CD34+ cord blood cells were purchased from AllCells. A549 cells, U-2 OS cells, Neuro-2a (N2A) cells were purchased from ATCC. MEF feeder cells and ROCK inhibitor Y-27632 were SaveFull Paper
2015Kempema, AM;Widen, JC;Hexum, JK;Andrews, TE;Wang, D;Rathe, SK;Meece, FA;Noble, KE;Sachs, Z;Largaespada, DA;Harki, DA;Synthesis and antileukemic activities of C1-C10-modified parthenolide analoguesBioorganic & medicinal chemistry26088334Frozen human mononuclear bone marrow cells were purchased from AllCells (Cat. #ABM011F). These bone marrow cells were from two donors (#5630 [Lot #BM4565] and #4887 [Lot # SaveFull Paper
2015Saraf, S;Araki, H;Petro, B;Park, Y;Taioli, S;Yoshinaga, KG;Koca, E;Rondelli, D;Mahmud, N;Ex vivo expansion of human mobilized peripheral blood stem cells using epigenetic modifiersTransfusion25363624Growth factor-mobilized human MPB or BM cells were obtained from healthy donors either from a commercially available source (AllCells, LLC, Emeryville, CA) or from aliquots of SaveFull Paper
2015Geiger, A;Walker, A;Nissen, E;Human fibrocyte-derived exosomes accelerate wound healing in genetically diabetic miceBiochemical and biophysical research communications26454169Human donor fibrocytes were obtained from leukapheresis packs (AllCells), as previously reported for experiments requiring a high yield of these cells [5], [16], [28]. Viable CD45+[CD3/CD19/CD20/CRTH2]−CD16−CD115−CD11b+CD34+ fibrocytes were enumerateFull Paper
2015Takahashi, T;Katano, I;Ito, R;Ito, M;Visualization of the human CD4⁺ T-cell response in humanized HLA-DR4-expressing NOD/Shi-scid/γc(null) (NOG) mice by retrogenic expression of the human TCR geneBiochemical and biophysical research communications254625652.2. CD34+ hematopoietic stem cells Umbilical CD34+ cells were obtained from AllCells (Alameda, CA). 2.3. DNA constructs BLG-specific CD4+ T-cell clones (HA5.7 and YA4) were kindly provided by Dr. Kondo [14]. The TCRα and β genes were isolated and tFull Paper
2015Antebi, B;Zhang, Z;Wang, Y;Lu, Z;Chen, XD;Ling, J;Stromal-cell-derived extracellular matrix promotes the proliferation and retains the osteogenic differentiation capacity of mesenchymal stem cells on three-dimensional scaffoldsTissue engineering. Part C, Methods24965227Primary bone-marrow-derived mononuclear cells (MNCs) from human donors 20-30 years of age were purchased from AllCells, LLC (Emeryville, CA). MNCs were cultured on standard SaveFull Paper
2015Akiyama, H;Kobayashi, A;Ichimura, M;Tone, H;Nakatani, M;Inoue, M;Tojo, A;Kagami, H;Comparison of manual and automated cultures of bone marrow stromal cells for bone tissue engineeringJournal of bioscience and bioengineering25907575Fresh human bone marrows, obtained from three healthy donors (25- and 28-year-old males and a 38-year-old female), were purchased from AllCells (Emeryville, CA, USA). Each bone marrow was divided into two 20-mL groups, and the manual and automated prFull Paper
2015Natu, VP;Dubey, N;Loke, GC;Tan, TS;Ng, WH;Yong, CW;Cao, T;Rosa, V;Bioactivity, physical and chemical properties of MTA mixed with propylene glycolJournal of applied oral science : revista FOB26398513paration of MTA conditioned media The use of human dental pulp stem cells in this study was approved by the Institutional Review Board/NUS (Approval Number: NUS 2094). DPSCs between passages 3 and 6 (Allcells, Alameda, California, USA) were used to eFull Paper
2015Musa, M;Mohd Ali, K;Kannan, TP;Azlina, A;Omar, NS;Chatterji, A;Mokhtar, KI;Effects of Perivitelline Fluid Obtained from Horseshoe Crab on The Proliferation and Genotoxicity of Dental Pulp Stem CellsCell journal26199904xicity was evaluated based on the chromosome aberration (CA) and Ames tests. Materials and Methods Study design This was an in vitro experimental study carried out on DPSCs. Cell culture DPSCs from AllCells (USA, cat no. DP004F) were cultured in meFull Paper
2015Elorza, MT;Human cytomegalovirus genome replication and maintenance during latent infectionThesisHuman CD14 (+) cells purchased (Lonza and ReachBio) or isolated from cord blood were maintained in Iscove DMEM (Hyclone) supplemented with 20% heatinactivated FBS (Atlanta Biologicals), 50 ng/mL M-CSF, 50 ng/mL stem cell factor (SCF), 50 ng/mL G-CSF,Full Paper
2015Kamocki, K;Nör, JE;Bottino, MC;Effects of ciprofloxacin-containing antimicrobial scaffolds on dental pulp stem cell viability-In vitro studiesArchives of oral biology26042622% penicillin-streptomycin (Sigma-Aldrich) placed in a humidified incubator at 37°C, with 5% CO2, was used to culture human dental pulp stem cells (hDPSC, AllCells LLC, Alameda, CA, USA) obtained from permanent third molars. 17,18 The medium was changFull Paper
2015Guo, J;Wang, Q;Wai, D;Zhang, QZ;Shi, SH;Le, AD;Shi, ST;Yen, SL;Visible red and infrared light alters gene expression in human marrow stromal fibroblast cellsOrthodontics & craniofacial research25865533Commercially available human bone marrow aspirates from two healthy adult donors were purchased from AllCells (Emeryville, CA). Human bone marrow stem cells (HBMSCs) were isolated from the aspirates following published protocols10, 11 and then culturFull Paper
2015Thys, RG;Lehman, CE;Pierce, LC;Wang, YH;Environmental and chemotherapeutic agents induce breakage at genes involved in leukemia-causing gene rearrangements in human hematopoietic stem/progenitor cellsMutation research26163765ot been previously connected to leukemogenesis. 2. Materials and methods 2.1. Cells and culture conditions Experiments were performed using primary human CD34+ bone marrow stem and progenitor cells (ALLCELLS), grown in Hematopoietic Growth Medium (LFull Paper
2015Low, A;Mohd Yusof, H;Reza, F;Abdullah Nurul, A;Sritharan, S;Haania Zain Ali, N;Subhi Azeez, H;Husein, A;Gypsum-based biomaterials: Evaluation of physical and mechanical properties, cellular effects and its potential as a pulp linerDental materials journal26235719SHED (AllCells, Chicago, IL, USA) were cultivated under sterile conditions in culture flasks containing growth media in a controlled condition at 37°C under a 5% CO2 humidified atmosphere. The culture media consisted of 10% heat-inactivated fetal bovFull Paper
2015Zhan, H;Ciano, K;Dong, K;Zucker, S;Targeting glutamine metabolism in myeloproliferative neoplasmsBlood cells, molecules & diseases26227854Peripheral blood CD34 + cells from healthy controls were purchased from AllCells . Peripheral blood CD34 + cells from healthy controls were purchased from AllCells . SaveFull Paper
2015Ciaffoni, F;Cassella, E;Varricchio, L;Massa, M;Barosi, G;Migliaccio, AR;Activation of non-canonical TGF-β1 signaling indicates an autoimmune mechanism for bone marrow fibrosis in primary myelofibrosisBlood cells, molecules & diseases25703685Signalling profiling was performed on mononuclear cells obtained from BM (n=3) and spleen (n=6) of patients, normal BM (n=3) (ABM008F-BM3366, ABM008F-BM3527 and ABM008F-BM3600, ALLCELLS, Emeryville, CA) and spleen (n=3) from males (30-years old) whoFull Paper
2015Nazarian, AA;Archibeque, IL;Nguyen, YH;Wang, P;Sinclair, AM;Powers, DA;Characterization of bispecific T-cell Engager (BiTE) antibodies with a high-capacity T-cell dependent cellular cytotoxicity (TDCC) assayJournal of biomolecular screening25477202Normal peripheral blood (NPB) was collected via leukapheresis from normal healthy human donors who were nonsmokers and were not taking any allergy medication (AllCells, SaveFull Paper
2015Suknuntha, K;Ishii, Y;Tao, L;Hu, K;McIntosh, BE;Yang, D;Swanson, S;Stewart, R;Wang, JYJ;Thomson, J;Slukvin, I;Discovery of survival factor for primitive chronic myeloid leukemia cells using induced pluripotent stem cellsStem cell research26561938Bone marrow mononuclear cells from CML patients in the chronic phase were purchased commercially (AllCells or Applied StemCells), or obtained from the patients at the University of Save;w specimens from CML patients in the chronic phase, and controFull Paper
2015Kanjevac, T;Milovanovic, M;Milosevic-Djordjevic, O;Tesic, Z;Ivanovic, M;Lukic, A;Cytotoxicity of glass ionomer cement on human exfoliated deciduous teeth stem cells correlates with released fluoride, strontium and aluminum ion concentrationsArchives of Biological SciencesHuman SHED purchased from AllCells, LLC (Emeryville, California, USA) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS, 100 I.U./mL penicillin G and 100 μg/mL streptomycin (Sigma-AldrichFull Paper
2015Gonçalves Silva, I;Gibbs, BF;Bardelli, M;Varani, L;Sumbayev, VV;Differential expression and biochemical activity of the immune receptor Tim-3 in healthy and malignant human myeloid cellsOncotarget26413815e highest grade of purity and available commercially unless otherwise stated. Primary human AML cells Primary human AML mononuclear blasts (AML-PB001F, newly diagnosed/untreated) were purchased from AllCells (Alameda, CA, USA) and handled in accordaFull Paper
2015Gibbs, BF;Gonçalves Silva, I;Prokhorov, A;Abooali, M;Yasinska, IM;Casely-Hayford, MA;Berger, SM;Fasler-Kan, E;Sumbayev, VV;Caffeine affects the biological responses of human hematopoietic cells of myeloid lineage via downregulation of the mTOR pathway and xanthine oxidase activityOncotarget26384306For this purpose we used primary acute myeloid leukaemia cells AML-PB001F purchased from AllCells (Alameda, CA, USA). Cells were exposed to 0.1 μg/ml SCF (these cells express SaveFull Paper
2015Wang, Y;Li, JX;Wang, YQ;Miao, ZH;Tanshinone I inhibits tumor angiogenesis by reducing Stat3 phosphorylation at Tyr705 and hypoxia-induced HIF-1α accumulation in both endothelial and tumor cellsOncotarget26202747ere obtained from the French National Institute of Health and Medical Research (INSERM, FR), and cultured with complete MCDB131 medium (Invitrogen, Carlsbad, CA, USA). HUVEC cells were purchased from AllCells (Shanghai, China). Human breast cancer MCFull Paper
2015Liu, K;Liu, X;Peng, Z;Sun, H;Zhang, M;Zhang, J;Liu, S;Hao, L;Lu, G;Zheng, K;Gong, X;Wu, D;Wang, F;Shen, L;Retargeted human avidin-CAR T cells for adoptive immunotherapy of EGFRvIII expressing gliomas and their evaluation via optical imagingOncotarget26124178To isolate peripheral blood mononuclear Cells (PBMC), the red blood cells and platelets were removed with PBMC isolation kits according to the manufacturer's instruction (AllCells, SaveFull Paper
2015Ao, JY;Chai, ZT;Zhang, YY;Zhu, XD;Kong, LQ;Zhang, N;Ye, BG;Cai, H;Gao, DM;Sun, HC;Robo1 promotes angiogenesis in hepatocellular carcinoma through the Rho family of guanosine triphosphatases' signaling pathwayTumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine26022159Robo1 is a member of the Robo immunoglobulin superfamily of proteins, and it plays an important role in angiogenesis and cancer. In this study, we investigate the role of roundabout 1 SaveFull Paper
2015Patel, P;Mahmud, D;Park, Y;Yoshinaga, K;Mahmud, N;Rondelli, D;Clinical grade isolation of regulatory T cells from G-CSF mobilized peripheral blood improves with initial depletion of monocytesAmerican journal of blood research27069755Tregs were purified from G-PBSC (AllCells) obtained from healthy donors. In some cases CD34 + cells were initially isolated on a ClinMACS device (Miltenyi) by positive selection using SaveFull Paper
2015Woodell-May, JE;Tan, ML;King, WJ;Swift, MJ;Welch, ZR;Murphy, MP;McKale, JM;Characterization of the Cellular Output of a Point-of-Care Device and the Implications for Addressing Critical Limb IschemiaBioResearch open access26634187s collected from the iliac crest from healthy financially compensated subjects who qualified for blood donation as per AABB standards. Aspiration was performed by Poietics-Cambrex (Gaithersburg, MD), AllCells (Alameda, CA), and HemaCare (Van Nuys, CAFull Paper
2015Yao, J;Song, A;Ruan, Z;Zhou, L;Liu, P;Zhu, H;Gong, H;Dong, S;Xu, Y;Jiang, E;Pang, A;Feng, S;Han, M;[Vascular endothelial injury induced by anti-endothelial cell antibody in allogeneic hematopoietic stem cell transplantation]Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi26134010FBS)及BCA定量试剂盒购自美国Thermo Scientific公司;辣 过氧化酶 记的羊抗人IgG购自 美国KPL公司;四甲基联苯胺(TMB)购自美国Tiangen公司;HUVEC及EC完全培养基购自美国Allcells SaveFull Paper
2015Du, C;Weng, X;Hu, W;Lv, Z;Xiao, H;Ding, C;Gyabaah, OA;Xie, H;Zhou, L;Wu, J;Zheng, S;Hypoxia-inducible MiR-182 promotes angiogenesis by targeting RASA1 in hepatocellular carcinomaJournal of experimental & clinical cancer research : CR26126858The HUVECs were obtained from Allcells (Shanghai, China allcells.biomart.cn) and maintained with Serum Free Medium for Endothelial Cells (SFM, Invitrogen) supplemented with 20 SaveFull Paper
2015Kawase, T;Tanaka, T;Okuda, K;Tsuchimochi, M;Oda, M;Hara, T;Quantitative single-cell motility analysis of platelet-rich plasma-treated endothelial cells in vitroCytoskeleton (Hoboken, N.J.)25845465HUVECs were obtained from AllCells, LLC (Emeryville, CA) and maintained in endothelial cell medium supplemented with growth factors (HuMedia-EB2; KURABO, Osaka, Japan) [Kobayashi et al., 2012]. For the wound healing assay, HUVECs were seeded into douFull Paper
2015Fung, C;Mohamad, H;Hashim, S;Htun, A;Ahmad, A;Proliferative Effect of Malaysian Propolis on Stem Cells from Human Exfoliated Deciduous Teeth: An In vitro StudyBritish Journal of Pharmaceutical ResearchThe frozen commercial SHED purchased from AllCells (USA) was retrieved and cultured in alpha minimal essential media (α-MEM) (Gibco, Invitrogen, USA) supplemented with 15% SaveFull Paper
2015Miura, Y;Ichinohe, T;Maekawa, T;Human Mesenchymal Stromal/Stem Cell-Mediated Bone Marrow OrganizationJapanese Journal of Transfusion and Cell TherapyIn this model, MSCs isolated from bone marrow samples (purchased from AllCells, Emeryville, CA, USA) through the conventional adhesion selection method (BM-MSCs, Fig. A) were SaveFull Paper
2015Kanazawa, T;Shiba, N;Aizawa, A;Okuno, H;Tamura, K;Tsukada, S;Kumamoto, T;Yamada, S;Kobayashi, Y;Arakawa, H;Nelarabine Resistance of Childhood T-Cell Lymphoblastic Leukemia/Lymphoma CellsThe Kitakanto Medical JournalIn vitro NEL resistance was seen in half of the testedchildhoodT-ALLcells. ENT-1maypartlyact as a transporter of NEL, but not play a key role in AraC induced NEL resistance. This cell SaveFull Paper
2015Wu, M;Davis, R;Hatch, A;Portable microfluidic raman system for rapid, label-free early disease signature detectionSandia National Laboratories (SNL)Order blood from Allcells(Alameda, CA), get blood within 4 hours. 2. Morning before blood arrives: 3. Make 50 ml 1x BD lysis buffer (Cat no. 349202), store at 4C. 4. Make 100X stock solutions for stimulation: 1:10 dilution of LPS stock to make 10ug/mFull Paper
2015Saka, E;Image registration and visualization of in situ gene expression images.University of LouisvilleAllcells are built out of similar materials (including an organism's DNA) and function insimilar ways. Prokaryotic cells, such as bacteria and some single celled organisms, havesimple SaveFull Paper
2015Taha, S;Ling, J;Azmi, N;Kannan, T;Azlina, A;Mokhtar, K;Expression analysis of Notch signaling pathway molecules in SHED cultured in keratinocyte growth mediumBrazilian Journal of Oral SciencesStem cells from human exfoliated deciduous teeth (SHED) (ALLCells, Alameda, CA, USA) were employed in the current study. SHED was cultured and maintained in T25 cm 2 culture SaveFull Paper
2015Neises, A;Su, R;Zhang, X;Reprogramming of Human Cord Blood CD34+ Cells into Induced MSCsSpringerBriefs in Stem Cellsof water and add 0.9 ml of 5 % EDTA. Adjust the pH to 7.2-7.4. Add water to bring the volume up to 500 ml and filter sterilize through a 0.2-µm filter. Store at 4 °C, but bring to room temperature before use. 4. Alternatively, cord blood CD34+ cellsFull Paper
2015Doss, J;The Erythrocyte Transcriptome: Global Characterization and Therapeutic ImplicationThesisMobilized Peripheral Blood CD34+ Cells (AllCells) were grown in StemLine II media containing Glutamine (Sigma) with 20% BIT (StemCell Technologies) and 1% Penicillin/Streptomycin (Gibco). On Days 0-7, cells were supplemented with growth factors IL-3Full Paper
2015Ferng, AS;Cardiac Organogenesis: 3D Bioscaffolds, Bioenergetics and RegenerationThesisHuman amniotic stem cells were purchased (AllCells, Alameda, CA) and cultured in Celprogen media (Celprogen, Torrance, CA) under standard cell culture conditions before reseeding on the membrane patches. AmSCs were seeded at 100,000 cells/cm2 per memFull Paper
2015Quarmyne, M;Protein Tyrosine Phosphatase Receptor Type S (PTPRS) Regulates Hematopoietic Stem Cell Self-RenewalThesisCenter according to an approved protocol by the Institutional Review Board of Duke University or the Institutional Biosafety Committee of University of California - Los Angeles. Frozen CB mononuclear cells (MNCs) were obtained from AllCells, LLC., AlFull Paper
2015Sanacora, S;Regulation of IL-8 expression by proteasome in human monocytes and macrophagesThesisU937 human promyeloid cells were obtained from American Type Culture Collection (ATCC; Rockville, MD, USA). Peripheral blood mononuclear cells (PBMC) from healthy human volunteers were purchased from AllCells (PB003F; Alameda, CA, USA). The cells werFull Paper
2015Uppal, H;Doudement, E;Mahapatra, K;Potential Mechanisms for Thrombocytopenia Development with Trastuzumab Emtansine (T-DM1) The Effect of T-DM1 on Platelets and MegakaryocytesClin Cancer ResHSCs (CD133+/CD34+) were purchased from ALLCELLS. Briefly, these HSCs were enriched from bone marrow isolates of healthy donors using positive selection for CD133 and CD34. CD133+/CD34+ cells were first expanded in cytokine-enriched hematopoietic expFull Paper
2015Gupta, MS;Development of a Carboxymethylcellulose Hydrogel System for Stem Cell-Based Nucleus Pulposus Tissue EngineeringThesisHuman MSCs were isolated from the bone marrow of a 37 year-old male (AllCells, Emeryville, CA) using enriched density gradient centrifugation. Briefly, a Human MSC Enrichment Cocktail (StemCell Technologies, Vancouver, BC, Canada) (Lee et al. 2005) wFull Paper
2015Leon, B;Discovery, Development, and Applications of Natural Products: Abyssomicin 2, Quinoline Amino Alcohols, and Fluorinated Bile AcidsThesisIn vitro model of HIV latency. Generation of latent infected cells was performed as previously described.9 Briefly, naive CD4+ T cells were purified by negative selection using PBMCs from healthy HIV negative donors obtained by leukapheresis (AllCellFull Paper
2015Kizilyer, A;The Role of Neurotrophin Signaling in Megakaryocyte Differentiation and Platelet ProductionThesisCD34+ cells isolated from human bone marrow (BM) or umbilical cord blood were purchased from Allcells (Emeryville, CA, USA), and differentiated according to the previously published protocol [185]. For inducing megakaryocyte progenitor cell differentFull Paper
2015UCOK, C;KARAAHMETOĞLU, O;UCOK, C;An analysis of the decision to extract third molar teethThesisMaterials and method: SHED (AllCells, Emeryville, CA; 1 9 105 cells in 2 ml culture medium) were seeded into six-well plates and cultured for 12 h. After the change of fresh medium, LPS (E. coli; 1 lg/ml) or saline (control) was added. Following 3, 1Full Paper
2015OZMERİC KURTULUŞ, N;Selin, A;CANKAL, D;Treatment of a Patient with Unilateral Cleft Lip and Palate with Free Gingival GreftThesisMaterials and method: SHED (AllCells, Emeryville, CA; 1 9 105 cells in 2 ml culture medium) were seeded into six-well plates and cultured for 12 h. After the change of fresh medium, LPS (E. coli; 1 lg/ml) or saline (control) was added. Following 3, 1Full Paper
2015Wang, X;Wei, W;Krzeszinski, JY;Wang, Y;Wan, Y;A Liver-Bone Endocrine Relay by IGFBP1 Promotes Osteoclastogenesis and Mediates FGF21-Induced Bone ResorptionCell metabolism26456333cells were pelleted by centrifugation at 10K rpm for 10min; supernatant was collected as liver-cell-derived conditioned medium (LCM) and used to treat osteoclast differentiation culture. Human PBMN cells (ReachBio) were differentiated into osteoclastFull Paper
2015Xiang, J;Wu, DC;Chen, Y;Paulson, RF;In vitro culture of stress erythroid progenitors identifies distinct progenitor populations and analogous human progenitorsBlood25608563Human bone marrow mononuclear cells were purchased from Reachbio (#033-200) and cultured in the stress erythropoiesis medium described above except that human growth SaveFull Paper
2015Jaramillo, MC;Briehl, MM;Batinic-Haberle, I;Tome, ME;Manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin acts as a pro-oxidant to inhibit electron transport chain proteins, modulate bioenergetics, and enhance the response to chemotherapy in lymphoma cellsFree radical biology & medicine25725417The manganese porphyrin, manganese (III) meso-tetrakis N-ethylpyridinium-2-yl porphyrin (MnTE-2-PyP 5+), acts as a pro-oxidant in the presence of intracellular H 2 O 2. Mitochondria Save;Normal human peripheral mononuclear blood cells (PMBC) were pFull Paper
2015Grandi, FC;Rosser, JM;Newkirk, SJ;Yin, J;Jiang, X;Xing, Z;Whitmore, L;Bashir, S;Ivics, Z;Izsvák, Z;Ye, P;Yu, YE;An, W;Retrotransposition creates sloping shores: a graded influence of hypomethylated CpG islands on flanking CpG sitesGenome research25995269hnology, Paul Ehrlich Institute, 63225 Langen, Germany 6 These authors contributed equally to this work. 7 Present addresses: Cancer Biology Program, Stanford University, Stanford, CA 94305, USA; 8 ReachBio LLC, Seattle, WA 98107, USA; 9 DepartmenFull Paper
2015Purushothaman, P;Thakker, S;Verma, SC;Transcriptome analysis of Kaposi's sarcoma-associated herpesvirus during de novo primary infection of human B and endothelial cellsJournal of virology25552714Human PBMCs (ReachBio, WA) were cultured in RPMI 1640 medium (HyClone) supplemented with 10% FBS, 2 mM l-glutamine, and penicillin-streptomycin (5 U/ml and 5 μg/ml, respectively). Human CD14+ cells were isolated from cord blood received from the ColoFull Paper
2015Panchanathan, R;Liu, H;Leung, YK;Ho, SM;Choubey, D;Bisphenol A (BPA) stimulates the interferon signaling and activates the inflammasome activity in myeloid cellsMolecular and cellular endocrinology26277401Cells lysates were prepared and analyzed for the levels of activated caspase-1 and the mature IL-1β as described (Veeranki et al., 2011). Human purified (CD14+) monocytes from a healthy male donor were purchased from ReachBio (Seattle, WA) and culturFull Paper
2015D'Alessandro, JS;Duffner, J;Pradines, J;Capila, I;Garofalo, K;Kaundinya, G;Greenberg, BM;Kantor, D;Ganguly, TC;Equivalent Gene Expression Profiles between Glatopa™ and Copaxone®PloS one26473741tiation of the experiment to minimize bias. All samples were tested on the same day to minimize the introduction of bias resulting from sample processing. First, APC 0.5 mL (naive Balb/C splenocytes, ReachBio LLC, Seattle, WA, USA) cell suspension (1Full Paper
2015Tarrant, JM;Dhawan, P;Singh, J;Zabka, TS;Clarke, E;DosSantos, G;Dragovich, PS;Sampath, D;Lin, T;McCray, B;La, N;Nguyen, T;Kauss, A;Dambach, D;Misner, DL;Diaz, D;Uppal, H;Preclinical models of nicotinamide phosphoribosyltransferase inhibitor-mediated hematotoxicity and mitigation by co-treatment with nicotinic acidToxicology mechanisms and methods25894564Human bone marrow (n = 3) (ReachBio, Seattle, WA) or cynomolgus monkey bone marrow During the conduct of this work, authors were employees of either Genentech or ReachBio. SaveFull Paper
2015Cook, G;Studies of the efficacy of Ribonucleotide Reductase inhibition in preclinical models of Acute Myeloid Leukemia in combination with standard and novel therapeuticsThesisCells were washed with PBS and resuspended in IMDM supplemented with 20% FBS, and placed in ColonyGel High Cytokine Formulation media (ReachBio). Experiments were performed in triplicate. Colonies were counted on or after day 7. Colonies of 8 or moreFull Paper
2015Thakker, ST;Dynamics of KSHV gene expression during de novo infection and the role of LANA in immune modulationThesisErle S. Robertson, were cultured in endothelial basal medium-2 (Lonza) supplemented with endothelial growth factor supplements (Lonza). Human PBMCs (ReachBio, WA) were cultured in RPMI 1640 medium (Hyclone) supplemented with 10% FBS, 2 mM L-glutamineFull Paper
2014Li, S;Tighe, SW;Nicolet, CM;Grove, D;Levy, S;Farmerie, W;Viale, A;Wright, C;Schweitzer, PA;Gao, Y;Kim, D;Boland, J;Hicks, B;Kim, R;Chhangawala, S;Jafari, N;Raghavachari, N;Gandara, J;Garcia-Reyero, N;Hendrickson, C;Roberson, D;Rosenfeld, J;Smith, T;Underwood, JG;Wang, M;Zumbo, P;Baldwin, DA;Grills, GS;Mason, CE;Multi-platform assessment of transcriptome profiling using RNA-seq in the ABRF next-generation sequencing studyNat. Biotechnol.25150835Department of Physiology and Biophysics, Weill Cornell Medical College, New York, New York, USA.++Vermont Cancer Center, University of Vermont, Burlington, Vermont, USA.++Keck School of Medicine, University of Southern California, Los Angeles, CalifoFull Paper
2014Graves, JD;Kordich, JJ;Huang, TH;Piasecki, J;Bush, TL;Sullivan, T;Foltz, IN;Chang, W;Douangpanya, H;Dang, T;O'Neill, JW;Mallari, R;Zhao, X;Branstetter, DG;Rossi, JM;Long, AM;Huang, X;Holland, PM;Apo2L/TRAIL and the death receptor 5 agonist antibody AMG 655 cooperate to promote receptor clustering and antitumor activityCancer Cell25043603Non-small-cell lung tumor samples were collected under Institutional Review Board approval for each institution, with appropriate informed consent, from surgical resections or after biopsy. Freshly resected human tissue samples were obtained from theFull Paper
2014Brissova, M;Aamodt, K;Brahmachary, P;Prasad, N;Hong, JY;Dai, C;Mellati, M;Shostak, A;Poffenberger, G;Aramandla, R;Levy, SE;Powers, AC;Islet microenvironment, modulated by vascular endothelial growth factor-A signaling, promotes β cell regenerationCell Metab.24561261Division of Diabetes, Endocrinology, and Metabolism, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232, USA. Electronic address: marcela.brissova@vanderbilt.edu.++Department of Molecular Physiology and Biophysics, VandFull Paper
2014Locke, D;Lynch, F;Bernstein, S;Siami-Namini, K;Yarranton, G;An IHC Screen for EphA3 Positive Myelofibrosis (MF) and Other Myeloproliferative Neoplasms (MPNs)BloodNeoplasms (MPNs). Darren Locke, PhD Darren Locke, PhD. 1. QualTek Molecular Laboratories, Newtown,. 1. Search for other works by this author on: This Site. PubMed MPNs. Disclosures. Locke:QualTek Molecular Labs: Employment. LynchFull Paper
2014Fecteau, JF;Corral, LG;Ghia, EM;Gaidarova, S;Futalan, D;Bharati, IS;Cathers, B;Schwaederlé, M;Cui, B;Lopez-Girona, A;Messmer, D;Kipps, TJ;Lenalidomide inhibits the proliferation of CLL cells via a cereblon/p21(WAF1/Cip1)-dependent mechanism independent of functional p53Blood24990888Blood samples were collected from CLL patients at the University of California San Diego Moores Cancer Center who satisfied diagnostic and immunophenotypic criteria for common B-cell CLL, and who provided written, informed consent, in compliance withFull Paper
2014Sugihara, K;Kobayashi, Y;Suzuki, A;Tamura, N;Motamedchaboki, K;Huang, CT;Akama, TO;Pecotte, J;Frost, P;Bauer, C;Jimenez, JB;Nakayama, J;Aoki, D;Fukuda, MN;Development of pro-apoptotic peptides as potential therapy for peritoneal endometriosisNat Commun25047118... wa (positive) and A431 (negative) cells. A hybridoma clone was subcloned by a limited dilution, establishing the 3B2 clone. Paraffin tissue sections endometriosis patients (_n_=35) were obtained from Folio Biosciences (Columbus, OH). A normal uteFull Paper
2014Ramesh, R;Kozhaya, L;McKevitt, K;Djuretic, IM;Carlson, TJ;Quintero, MA;McCauley, JL;Abreu, MT;Unutmaz, D;Sundrud, MS;Pro-inflammatory human Th17 cells selectively express P-glycoprotein and are refractory to glucocorticoidsJ. Exp. Med.24395888... sue samples. All experiments using human blood and tissue samples were conducted in accordance with IRB protocols approved by committees at Research Blood Components, New York Blood Center, OneBlood, Conversant Bio., and the University of Miami.Full Paper
2014Van Bortle, K;Nichols, MH;Li, L;Ong, CT;Takenaka, N;Qin, ZS;Corces, VG;Insulator function and topological domain border strength scale with architectural protein occupancyGenome Biol.24981874We are especially thankful to Drs Dale Dorsett, Hugo Bellen, Giovanni Bosco, and Jørgen Johansen for graciously sharing antibodies against Rad21, Barren, CAP-H2, and Chromator. We thank the Drosophila Genomic Resource Center (supported by NIH grant OFull Paper
2014Steffen, MM;Dearth, SP;Dill, BD;Li, Z;Larsen, KM;Campagna, SR;Wilhelm, SW;Nutrients drive transcriptional changes that maintain metabolic homeostasis but alter genome architecture in MicrocystisISME J24858783; Scientific, Waltham, MA, USA). Subsequent sample processing, including rRNA subtraction, library prep and sequencing, were performed by the HudsonAlpha Genomic Services Laboratory (Huntsville, AL, USA). RNA quality wasFull Paper
2014Picard, M;Zhang, J;Hancock, S;Derbeneva, O;Golhar, R;Golik, P;O'Hearn, S;Levy, S;Potluri, P;Lvova, M;Davila, A;Lin, CS;Perin, JC;Rappaport, EF;Hakonarson, H;Trounce, IA;Procaccio, V;Wallace, DC;Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional reprogrammingProc. Natl. Acad. Sci. U.S.A.25192935Center for Mitochondrial and Epigenomic Medicine, Children's Hospital of Philadelphia and the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, PA 19104;++School of Biological Sciences, The University of HongFull Paper
2014Smith, DC;Eisenberg, PD;Manikhas, G;Chugh, R;Gubens, MA;Stagg, RJ;Kapoun, AM;Xu, L;Dupont, J;Sikic, B;A phase I dose escalation and expansion study of the anticancer stem cell agent demcizumab (anti-DLL4) in patients with previously treated solid tumorsClin. Cancer Res.25324140In addition, control plasma and whole blood samples were sourced and collected (Conversant Bio) using the same methods described above from 8 cancer patients who were not treated with demcizumab. Affymetrix human gene chip U133 Plus 2.0 arrays were uFull Paper
2014Gee, HY;Ashraf, S;Wan, X;Vega-Warner, V;Esteve-Rudd, J;Lovric, S;Fang, H;Hurd, TW;Sadowski, CE;Allen, SJ;Otto, EA;Korkmaz, E;Washburn, J;Levy, S;Williams, DS;Bakkaloglu, SA;Zolotnitskaya, A;Ozaltin, F;Zhou, W;Hildebrandt, F;Mutations in EMP2 cause childhood-onset nephrotic syndromeAm. J. Hum. Genet.24814193Division of Nephrology, Department of Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USA.++Division of Nephrology, Department of Medicine, Boston Children's Hospital and Harvard Medical School, Boston, MA 02115, USFull Paper
2014Gee, HY;Otto, EA;Hurd, TW;Ashraf, S;Chaki, M;Cluckey, A;Vega-Warner, V;Saisawat, P;Diaz, KA;Fang, H;Kohl, S;Allen, SJ;Airik, R;Zhou, W;Ramaswami, G;Janssen, S;Fu, C;Innis, JL;Weber, S;Vester, U;Davis, EE;Katsanis, N;Fathy, HM;Jeck, N;Klaus, G;Nayir, A;Rahim, KA;Al Attrach, I;Al Hassoun, I;Ozturk, S;Drozdz, D;Helmchen, U;O'Toole, JF;Attanasio, M;Lewis, RA;Nürnberg, G;Nürnberg, P;Washburn, J;MacDonald, J;Innis, JW;Levy, S;Hildebrandt, F;Whole-exome resequencing distinguishes cystic kidney diseases from phenocopies in renal ciliopathiesKidney Int.24257694Division of Nephrology, Department of Medicine, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts, USA.++Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan, USA.++MRC Human GeneticsFull Paper
2014Rosenfeldt, MT;Bell, LA;Long, JS;O'Prey, J;Nixon, C;Roberts, F;Dufès, C;Ryan, KM;E2F1 drives chemotherapeutic drug resistance via ABCG2Oncogene24276245Biotechnology (St Louis, MO, USA). The ChIP Assay Kit was from Millipore (Watford, UK) and the human lung cancer tissue microarray was from Folio Biosciences (Columbus, OH, USA). Plasmids. pTRE-E2F1 constructs were generatedFull Paper
2014Wildsmith, KR;Schauer, SP;Smith, AM;Arnott, D;Zhu, Y;Haznedar, J;Kaur, S;Mathews, WR;Honigberg, LA;Identification of longitudinally dynamic biomarkers in Alzheimer's disease cerebrospinal fluid by targeted proteomicsMol Neurodegener24902845... ap in clinical biomarker development. Methods Source of CSF CSF was purchased from Bioreclamation, LLC (Hicksville, NY) (pooled cynomolgus monkey and pooled human CSF used in discovery experiments), Folio Biosciences (Columbus, OH) (individual lFull Paper
2014Farber, CR;Reich, A;Barnes, AM;Becerra, P;Rauch, F;Cabral, WA;Bae, A;Quinlan, A;Glorieux, FH;Clemens, TL;Marini, JC;A novel IFITM5 mutation in severe atypical osteogenesis imperfecta type VI impairs osteoblast production of pigment epithelium-derived factorJ. Bone Miner. Res.24519609Exome sequencing was performed by the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology (Huntsville, AL, USA). Briefly, gDNA (1 to 2 μg) was fragmented and subjected to exome enrichment using the Nimblegen SeqCap EZ Human Exome LibrFull Paper
2014Shipitsin, M;Small, C;Choudhury, S;Giladi, E;Friedlander, S;Nardone, J;Hussain, S;Hurley, AD;Ernst, C;Huang, YE;Chang, H;Nifong, TP;Rimm, DL;Dunyak, J;Loda, M;Berman, DM;Blume-Jensen, P;Identification of proteomic biomarkers predicting prostate cancer aggressiveness and lethality despite biopsy-sampling errorBr. J. Cancer25032733Acquisition, processing, quality control, and annotation of FFPE prostate cancer tissue blocks. A set of FFPE human prostate cancer tissue blocks with clinical annotations and long-term patient outcome information was acquired from Folio BiosciencesFull Paper
2014Hermetz, KE;Newman, S;Conneely, KN;Martin, CL;Ballif, BC;Shaffer, LG;Cody, JD;Rudd, MK;Large inverted duplications in the human genome form via a fold-back mechanismPLoS Genet.24497845SureSelect capture and Illumina HiSeq sequencing were performed at Hudson Alpha Genomic Services Lab (http://www.hudsonalpha.org/gsl/). After NGS, we aligned 100-bp paired-end reads from fastq files to the GRC37/hg19 reference genome using Burrows-WhFull Paper
2014Campbell, VT;Nadesan, P;Ali, SA;Wang, CY;Whetstone, H;Poon, R;Wei, Q;Keilty, J;Proctor, J;Wang, LW;Apte, SS;McGovern, K;Alman, BA;Wunder, JS;Hedgehog pathway inhibition in chondrosarcoma using the smoothened inhibitor IPI-926 directly inhibits sarcoma cell growthMol. Cancer Ther.24634412Formalin-fixed, paraffin-embedded human chondrosarcoma tissue microarrays (TMA) were purchased from Folio Biosciences for cilia staining. Before staining, all slides were placed in a 60 C oven and baked for 1 hour. Sections were deparaffinized in xylFull Paper
2014Folcik, VA;Garofalo, M;Coleman, J;Donegan, JJ;Rabbani, E;Suster, S;Nuovo, A;Magro, CM;Di Leva, G;Nuovo, GJ;Idiopathic pulmonary fibrosis is strongly associated with productive infection by herpesvirus saimiriMod. Pathol.242328641] The Ohio State University (OSU) at Marion, Marion, OH, USA [2] OSU Computer Science and Engineering, Marion, OH, USA [3] OSU Innovation Group for the Study of Complexity in Human, Natural, and Engineered Systems, Marion, OH, USA.++Satellite LaboraFull Paper
2014Lee, CW;Zhan, Q;Lezcano, C;Frank, MH;Huang, J;Larson, AR;Lin, JY;Wan, MT;Lin, PI;Ma, J;Kleffel, S;Schatton, T;Lian, CG;Murphy, GF;Nestin depletion induces melanoma matrix metalloproteinases and invasionLab. Invest.25365206An additional 153 cases of primary and metastatic melanomas demonstrating clear nodular or infiltrative growth patterns were evaluated for nestin expression in melanoma tissue microarrays (TMA) purchased from Folio Bio (Powell, OH, USA), US Biomax (RFull Paper
2014Johansson, H;Orjalo, A;Coassin, S;Yin, R;Abstract 3399: Detection and validation of novel RNA cancer biomarkers by single molecule RNA fluorescence in situ hybridization (smRNA FISH)Molecular and Cellular BiologyMethods Mol Biol. Ron Cook - Founder, President and CEO Marc Beal - Director of Corporate Development Peter Coassin - Director of Advanced Technology Development Entire Stellaris Team OPKOLab Folio Biosciences #3399 A B A B SK-BR-3 MCF7 T47DFull Paper
2014Hornby, PJ;Cooper, PR;Kliwinski, C;Ragwan, E;Mabus, JR;Harman, B;Thompson, S;Kauffman, AL;Yan, Z;Tam, SH;Dorai, H;Powers, GD;Giles-Komar, J;Human and non-human primate intestinal FcRn expression and immunoglobulin G transcytosisPharm. Res.24072267Paraffin-imbedded 5 μm sections were cut from full-thickness intestine harvested from monkey and human donors, and from human tissue bank (MTB-311 and MTB-316; Qualtek Molecular Labs, Goleta, CA) and dried on glass slides (60°C). Steam heat induced eFull Paper
2014Jane, EP;Premkumar, DR;Morales, A;Foster, KA;Pollack, IF;Inhibition of phosphatidylinositol 3-kinase/AKT signaling by NVP-BKM120 promotes ABT-737-induced toxicity in a caspase-dependent manner through mitochondrial dysfunction and DNA damage response in established and primary cultured glioblastoma cellsJ. Pharmacol. Exp. Ther.24741074The human glioma cell lines U87 (PTEN deleted, p53 wild type) and T98G (PTEN mutant, p53 mutant) were purchased from the ATCC (Manassas, VA). LN18 (PTEN wild type, p53 mutant), LN229 (PTEN wild type, p53 mutant), and LNZ308 (PTEN deleted, p53 deletedFull Paper
2014Hassan, F;Xu, X;Nuovo, G;Killilea, DW;Tyrrell, J;Da Tan, C;Tarran, R;Diaz, P;Jee, J;Knoell, D;Boyaka, PN;Cormet-Boyaka, E;Accumulation of metals in GOLD4 COPD lungs is associated with decreased CFTR levelsRespir. Res.24957904... ell@osumc.edu Boyaka Prosper N 1 boyaka.1@osu.edu Cormet-Boyaka Estelle 1 boyaka.2@osu.edu 1Department of Veterinary Biosciences, The Ohio State University, 1925 Coffey Road, Columbus, OH 43210, USA 2Phylogeny Inc., Columbus, OH, USA 3Nutrition aFull Paper
2014Burger, KL;Learman, BS;Boucherle, AK;Sirintrapun, SJ;Isom, S;Díaz, B;Courtneidge, SA;Seals, DF;Src-dependent Tks5 phosphorylation regulates invadopodia-associated invasion in prostate cancer cellsProstate24174371To conduct an immunohistochemical analysis of Tks5 protein, we purchased a high density prostate tumor microarray (Folio Bioscience, Powell, OH) containing 92, duplicate cases of formalin‐fixed human prostatic adenocarcinoma and 20 cases of normal anFull Paper
2014Gómez-Díaz, E;Rivero, A;Chandre, F;Corces, VG;Insights into the epigenomic landscape of the human malaria vector Anopheles gambiaeFront Genet25177345Total RNA was extracted from fresh mosquito tissues (~25 midguts and ~ 50 salivary glands) using the mirVana™ RNA Isolation Kit (Ambion®) according to the manufacturer's protocol. RNA concentration was quantified using a Qubit® 2.0 Fluorometer, and RFull Paper
2014Xu, J;Zhao, Z;Zhang, X;Zheng, X;Li, J;Jiang, Y;Kuang, Y;Zhang, Y;Feng, J;Li, C;Yu, J;Li, Q;Zhu, Y;Liu, Y;Xu, P;Sun, X;Development and evaluation of the first high-throughput SNP array for common carp (Cyprinus carpio)BMC Genomics24762296;... es were collected and DNA was extracted using a DNeasy Blood & Tissue Kit (Qiagen, Shanghai, China). The samples are listed in Table 1. DNA library preparation and sequencing were carried out at the HudsonAlpha Genomic Services Laboratory (HuntsFull Paper
2014Gruver, AM;Liu, L;Vaillancourt, P;Yan, SC;Cook, JD;Roseberry Baker, JA;Felke, EM;Lacy, ME;Marchal, CC;Szpurka, H;Holzer, TR;Rhoads, EK;Zeng, W;Wortinger, MA;Lu, J;Chow, CK;Denning, IJ;Beuerlein, G;Davies, J;Hanson, JC;Credille, KM;Wijayawardana, SR;Schade, AE;Immunohistochemical application of a highly sensitive and specific murine monoclonal antibody recognising the extracellular domain of the human hepatocyte growth factor receptor (MET)Histopathology25039923both acquired from US Biomax, Rockville, MD, USA), and 67 NSCLC whole‐tissue specimens purchased from Asterand (Detroit, MI, USA), US Biomax, Capital Biosciences, Cybridi (Gaithersburg, MD, USA), Indivumed (Baltimore, MD, USA), Conversant Bio (HuntsvFull Paper
2014Li, C;Beck, BH;Peatman, E;Nutritional impacts on gene expression in the surface mucosa of blue catfish (Ictalurus furcatus)Dev. Comp. Immunol.24378224; For each timepoint, equal amounts of RNA from skin and gill were pooled together to generate 3 pooled replicates for RNA-seq library construction. RNA-seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (HunFull Paper
2014Ehrhardt, N;Bedoya, C;Péterfy, M;Embryonic viability, lipase deficiency, hypertriglyceridemia and neonatal lethality in a novel LMF1-deficient mouse modelNutr Metab (Lond)25302068RT-PCR. p6. CGTGGAGCTTCTTGTGCCT. qPCR. p7. GGATCTGCAGCACTCCAT. qPCR. 36B4-f. CACTGGTCTAGGACCCGAGAAG. qPCR. 36B4-r. GGTGCCTCTGGAGATTTTCG. qPCR. RNA analysis. In situ hybridization was carried out by Phylogeny Inc. as described [11]Full Paper
2014Choudhary, A;Mambo, E;Sanford, T;Boedigheimer, M;Twomey, B;Califano, J;Hadd, A;Oliner, KS;Beaudenon, S;Latham, GJ;Adai, AT;Evaluation of an integrated clinical workflow for targeted next-generation sequencing of low-quality tumor DNA using a 51-gene enrichment panelBMC Med Genomics25395014The 26 tumor specimens were all colon or rectal adenocarcinomas obtained from Asterand (Detroit, MI, USA). The remaining colon cancer FFPE tissue specimens come from stage II tumors that were acquired from FolioBio (Columbus, OH)Full Paper
2014Stavropoulos, SW;Sing, RF;Elmasri, F;Silver, MJ;Powell, A;Lynch, FC;Aal, AK;Lansky, AJ;Settlage, RA;Muhs, BE;, ;The DENALI Trial: an interim analysis of a prospective, multicenter study of the Denali retrievable inferior vena cava filterJ Vasc Interv Radiol25066514Full Paper
2014Day, K;Song, J;Absher, D;Targeted sequencing of large genomic regions with CATCH-SeqPLoS ONE25357200HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United States of America.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United States of America.++HudsonAlpha Institute for Biotechnology, Huntsville, Alabama, United StaFull Paper
2014Sun, L;Liu, S;Wang, R;Jiang, Y;Zhang, Y;Zhang, J;Bao, L;Kaltenboeck, L;Dunham, R;Waldbieser, G;Liu, Z;Identification and analysis of genome-wide SNPs provide insight into signatures of selection and domestication in channel catfish (Ictalurus punctatus)PLoS ONE25313648; protocol. Equal amounts of DNA (100 µg) from each individual were pooled for sequencing, one pool for each strain. Sequencing was conducted commercially at HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). GenomicFull Paper
2014Jiang, Y;Zhang, S;Xu, J;Feng, J;Mahboob, S;Al-Ghanim, KA;Sun, X;Xu, P;Comparative transcriptome analysis reveals the genetic basis of skin color variation in common carpPLoS ONE25255374;... nufacturer’s instructions. RNA quality was verified using a Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA). Equal amounts of high-quality RNA from each tissue were pooled and sent to HudsonAlpha Genomic Services Laboratory (HuntsvFull Paper
2014Jun, LS;Showalter, AD;Ali, N;Dai, F;Ma, W;Coskun, T;Ficorilli, JV;Wheeler, MB;Michael, MD;Sloop, KW;A novel humanized GLP-1 receptor model enables both affinity purification and Cre-LoxP deletion of the receptorPLoS ONE24695667For IHC, tissues and cells were fixed and processed for paraffin embedding using routine procedures as described previously [30]. Paraffin-embedded human pancreas samples were purchased from Folio Biosciences (Columbus, OH). IP-WB and mRNA ExpressionFull Paper
2014Luo, Y;Li, C;Landis, AG;Wang, G;Stoeckel, J;Peatman, E;Transcriptomic profiling of differential responses to drought in two freshwater mussel species, the giant floater Pyganodon grandis and the pondhorn Uniomerus tetralasmusPLoS ONE24586812; construction, Illumina sequencing and expression analysis. RNA-Seq library preparation and sequencing were carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were constructed withFull Paper
2014Li, SZ;Hu, YY;Zhao, J;Zhao, YB;Sun, JD;Yang, YF;Ji, CC;Liu, ZB;Cao, WD;Qu, Y;Liu, WP;Cheng, G;Fei, Z;MicroRNA-34a induces apoptosis in the human glioma cell line, A172, through enhanced ROS production and NOX2 expressionBiochem. Biophys. Res. Commun.243938442. Materials and methods. 2.1. Human samples and cell lines. Human glioma cancer tissue microarrays (Lot Number: ARY-HH0118-011-02) containing 60 glioma cancer specimens and 30 normal tissues were purchased from Folio Biosciences (Columbus, OH, USA)Full Paper
2014Yim, KM;Brewer, MS;Miller, CT;Gillespie, RG;Comparative transcriptomics of maturity-associated color change in Hawaiian spidersJ. Hered.25149253Extractions were shipped to the Genomic Services Lab at HudsonAlpha (Huntsville, AL) for library preparation and sequencing. Complementary DNA libraries were prepared from total RNA, and 50bp paired-end Illumina HiSeq 2000 RNA-seq was used to generatFull Paper
2014Shipitsin, M;Small, C;Giladi, E;Siddiqui, S;Choudhury, S;Hussain, S;Huang, YE;Chang, H;Rimm, DL;Berman, DM;Nifong, TP;Blume-Jensen, P;Automated quantitative multiplex immunofluorescence in situ imaging identifies phospho-S6 and phospho-PRAS40 as predictive protein biomarkers for prostate cancer lethalityProteome Sci25075204In total, we procured and tested 508 unique prostatectomy samples with lethal outcome annotation available (Folio Biosciences, Powell, OH). Of these, 418 passed quality testing and were used for our TMA (Additional file 1: Table S2). Figure 2 FigureFull Paper
2014Drogemöller, BI;Niehaus, DJ;Chiliza, B;van der Merwe, L;Asmal, L;Malhotra, AK;Wright, GE;Emsley, R;Warnich, L;Patterns of variation influencing antipsychotic treatment outcomes in South African first-episode schizophrenia patientsPharmacogenomics24444409gDNA from the 11 individuals was extracted from venous blood using the QIAamp DNA Blood Maxi Kit (Qiagen, Germany), accord - ing to the manufacturer’s instructions. There - after, the gDNA samples were sent to the HudsonAlpha Genomics Service LaboraFull Paper
2014Zhang, J;Vernes, JM;Ni, J;Nelson, C;Wong, A;Chen, ST;Asundi, A;Vandlen, R;Meng, YG;Real-time immuno-polymerase chain reaction in a 384-well format: detection of vascular endothelial growth factor and epidermal growth factor-like domain 7Anal. Biochem.25013989CAL-12T cell line was purchased from German Collection of Microorganisms and Cell Cultures (Braunschweig, Germany). Human cancer tissues were purchased from ConversantBio (Huntsville, AL) or Avaden BioSciences (Scarsdale, NY)Full Paper
2014Aycock, KI;Campbell, RL;Manning, KB;Sastry, SP;Shontz, SM;Lynch, FC;Craven, BA;A computational method for predicting inferior vena cava filter performance on a patient-specific basisJ Biomech Eng24805200Full Paper
2014Sarran-Armogan, L;Prospective isolation of human myeloerythroid progenitors in fetal liver and embryonic stem cell differentiation culturesThesisEinstein Fetal Tissue repository under IRB approved protocol. Human cord blood was obtained from the Obstetric Department at the Weiler Hospital of the Albert Einstein College of Medicine under an IRB approved protocol. Human bone marrow CD34+ cellsFull Paper
2014Miyake, M;Lawton, A;Goodison, S;Urquidi, V;Rosser, CJ;Chemokine (C-X-C motif) ligand 1 (CXCL1) protein expression is increased in high-grade prostate cancerPathol. Res. Pract.24252309High-density commercial tissue microarrays (TMA) (ARY-HH0185, Foliobio, Powell, OH) constructed from formalin fixed, paraffin embedded clinical samples comprised the cohort of 110 patients (90 prostate cancer and 20 benign prostates without evidenceFull Paper
2014Pollen, AA;Nowakowski, TJ;Shuga, J;Wang, X;Leyrat, AA;Lui, JH;Li, N;Szpankowski, L;Fowler, B;Chen, P;Ramalingam, N;Sun, G;Thu, M;Norris, M;Lebofsky, R;Toppani, D;Kemp, DW;Wong, M;Clerkson, B;Jones, BN;Wu, S;Knutsson, L;Alvarado, B;Wang, J;Weaver, LS;May, AP;Jones, RC;Unger, MA;Kriegstein, AR;West, JA;Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortexNature biotechnology25086649For systems verification tests of capture efficiency, primary cells were obtained from splenocytes (AllCells, LLC PB003F) and peripheral blood mononuclear cells (AllCells, LLC PB003F) SaveFull Paper
2014Cohen, A;Regenerative potential of human CD34+ stem cells mobilized and enriched from bone marrowThesisselected from granulocyte colony-stimulating factor (G-CSF) (Amgen; Thousand Oaks, CA, USA) mobilized peripheral blood mononuclear cells (mPBMNCs) obtained from AllCells (Regimen E, Emeryville, CA). Healthy human donors were injected with G-CSF onceFull Paper
2014Koellmer, M;Multilineage differentiation of mesenchymal stem cells for cell-based tissue engineeringThesisHuman bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque TM PLUS solution and RosetteSep® Human Mesenchymal Stem Cell Enrichment Cocktail (Stem Cell TechnologFull Paper
2014Lu, M;Xia, L;Li, Y;Wang, X;Hoffman, R;The orally bioavailable MDM2 antagonist RG7112 and pegylated interferon α 2a target JAK2V617F-positive progenitor and stem cellsBlood24869939human CD34+ cell selection kit (StemCell Technologies, Vancouver, BC, Canada). Normal human bone marrow (BM) mononuclear cells and CD34+ cells were purchased from AllCells (Emeryville, CA). The experiments for which the CD34+ cells from each of the 3Full Paper
2014Shi, J;Rose, EL;Singh, A;Hussain, S;Stagliano, NE;Parry, GC;Panicker, S;TNT003, an inhibitor of the serine protease C1s, prevents complement activation induced by cold agglutininsBlood24695853Human RBCs (hRBCs; blood type O negative; Allcells, Alameda, CA) were washed 3 times with gelatin veronal buffer (GVB; Complement Technology, Tyler, TX) and resuspended at 1 × 109 cells per milliliter in GVB plus 10 mM EDTA. To sensitize hRBCs with CFull Paper
2014Adamia, S;Bar-Natan, M;Haibe-Kains, B;Pilarski, PM;Bach, C;Pevzner, S;Calimeri, T;Avet-Loiseau, H;Lode, L;Verselis, S;Fox, EA;Galinsky, I;Mathews, S;Dagogo-Jack, I;Wadleigh, M;Steensma, DP;Motyckova, G;Deangelo, DJ;Quackenbush, J;Tenen, DG;Stone, RM;Griffin, JD;NOTCH2 and FLT3 gene mis-splicings are common events in patients with acute myeloid leukemia (AML): new potential targets in AMLBlood24574459Our previous studies revealed an increase in alternative splicing of multiple RNAs in cells from patients with acute myeloid leukemia (AML) compared with CD34 + bone marrow cells SaveFull Paper
2014Krow-Lucal, ER;Kim, CC;Burt, TD;McCune, JM;Distinct functional programming of human fetal and adult monocytesBlood24518760Adult bone marrow samples were obtained from healthy donors (AllCells and Lonza Group). Both adult and fetal mononuclear cells were isolated by density centrifugation of a Ficoll- SaveFull Paper
2014Shi, Q;Kuether, EL;Chen, Y;Schroeder, JA;Fahs, SA;Montgomery, RR;Platelet gene therapy corrects the hemophilic phenotype in immunocompromised hemophilia A mice transplanted with genetically manipulated human cord blood stem cellsBlood24269957Human cord blood (hCB) CD34+ cells were purchased from AllCells (Emeryville, CA) and transduced with 2bF8LV by using a protocol similar to that described in previous reports.10,15 The details are provided in the supplemental Data (available on the BlFull Paper
2014Thompson, A;Rasko, J;Hongeng, S;Kwiatkowski, J;Schiller, G;von Kalle, C;Cavazzana, M;Leboulch, P;Petrusich, A;Soni, S;Walters, M;Initial Results from the Northstar Study (HGB-204): A Phase 1/2 Study of Gene Therapy for β-Thalassemia Major Via Transplantation of Autologous Hematopoietic Stem Cells Transduced Ex Vivo with a Lentiviral βΑ-T87Q -Globin Vector (LentiGlobin BB305 Drug Product)BloodBackground: Hematopoietic stem cell (HSC) gene therapy has the potential to induce globin production and mitigate the need for blood transfusions in β-thalassemia major. Promising early results for 2 subjects with β0/βE -thalassemia major in the ongoFull Paper
2014Iwasa, M;Miura, Y;Fujishiro, A;Tamura, A;Sato, A;Yokota, A;Nakagawa, Y;Yoshioka, S;Hirai, H;Andoh, A;Ichinohe, T;Maekawa, T;Effects of Irradiation on the Functional Characteristics of Human Bone Marrow Mesenchymal Stromal/Stem CellsBloodBM samples were purchased from AllCells (Emeryville, CA). For the irradiation experiments, BM-MSCs were g-irradiated (Cesium-137) with various doses ranged from 2 to 12 Gy by a Save;Total-body irradiation is frequently used as a conditioning treatmFull Paper
2014Sahler, J;Modulation of Transcription Factors in Platelets and Platelet Microparticles Attenuates Inflammatory ResponsesThesisAdapted from previous procedures, 94 human CD34+ cord blood cells were purchased from Allcells (Emeryville, CA) and first plated at 2.5x105 cells/mL in IMDM supplemented with 20% BIT 9500, 0.8% Low Density Lipoprotein (L-8292, Sigma), 100ng/mL recombFull Paper
2014Li, S;Garrett-Bakelman, F;Perl, AE;Luger, SM;Zhang, C;To, BL;Lewis, ID;Brown, AL;D'Andrea, RJ;Ross, ME;Levine, R;Carroll, M;Melnick, A;Mason, CE;Dynamic evolution of clonal epialleles revealed by methcloneGenome biology25260792samples at two time points (diagnosis and relapse stages), enriched for myeloblast cells, were used in the experiments, and these samples were also compared to five CD34+ normal bone marrow samples (AllCells, Inc.). Institutional review board approvaFull Paper
2014Bartholdy, B;Christopeit, M;Will, B;Mo, Y;Barreyro, L;Yu, Y;Bhagat, TD;Okoye-Okafor, UC;Todorova, TI;Greally, JM;Levine, RL;Melnick, A;Verma, A;Steidl, U;HSC commitment-associated epigenetic signature is prognostic in acute myeloid leukemiaThe Journal of clinical investigation24487588Acute myeloid leukemia (AML) is characterized by disruption of HSC and progenitor cell differentiation. Frequently, AML is associated with mutations in genes encoding epigenetic SaveFull Paper
2014Schlinker, AC;In Vitro Platelet Production: Promoting Megakaryocyte Terminal Differentiation, Demonstrating Platelet Functionality, and Developing an Automated Process for Platelet HarvestThesisFigure 4.1. PLT-like particle (PLP) and proPLT production in X-VIVO 10 cultures. (A) Blood PLTs obtained from AllCells (top panels) and PLPs exposed to shear for 3 days after CD61+ selection on day 10 (bottom panels) were stained with anti-CD41 and aFull Paper
2014Accomando, WP;Wiencke, JK;Houseman, EA;Nelson, HH;Kelsey, KT;Quantitative reconstruction of leukocyte subsets using DNA methylationGenome biology24598480ummarizes the workflow carried out for all samples, derived from human whole blood, that were utilized in this work. Blood was obtained through an institutional review board-approved donor program at AllCells, LLC (Alameda, CA, USA) and research wasFull Paper
2014Liu, GH;Suzuki, K;Li, M;Qu, J;Montserrat, N;Tarantino, C;Gu, Y;Yi, F;Xu, X;Zhang, W;Ruiz, S;Plongthongkum, N;Zhang, K;Masuda, S;Nivet, E;Tsunekawa, Y;Soligalla, RD;Goebl, A;Aizawa, E;Kim, NY;Kim, J;Dubova, I;Li, Y;Ren, R;Benner, C;Del Sol, A;Bueren, J;Trujillo, JP;Surralles, J;Cappelli, E;Dufour, C;Esteban, CR;Belmonte, JCI;Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCsNature communications24999918Human BM CD34 + cells from a healthy individual were purchased from Allcells (Alameda, CA). FA patient BM cells were isolated from two FA patients bearing FANCA mutations ( SaveFull Paper
2014Green, MR;Vicente-Dueñas, C;Romero-Camarero, I;Long Liu, C;Dai, B;González-Herrero, I;García-Ramírez, I;Alonso-Escudero, E;Iqbal, J;Chan, WC;Campos-Sanchez, E;Orfao, A;Pintado, B;Flores, T;Blanco, O;Jiménez, R;Martínez-Climent, JA;Criado, FJ;Cenador, MB;Zhao, S;Natkunam, Y;Lossos, IS;Majeti, R;Melnick, A;Cobaleda, C;Alizadeh, AA;Sánchez-García, I;Transient expression of Bcl6 is sufficient for oncogenic function and induction of mature B-cell lymphomaNature communications24887457Bone marrow from two healthy donors (1 male, 1 female) were obtained purchased from AllCells, LLC. (Emeryville, CA) and sorted on a FACS ARIA II instrument as PI−, Lin−, CD34+, CD38−. Single cells were sorted into Terasaki plates containing alternatiFull Paper
2014Minn, I;Wang, H;Mease, RC;Byun, Y;Yang, X;Wang, J;Leach, SD;Pomper, MG;A red-shifted fluorescent substrate for aldehyde dehydrogenaseNature communications24759454(BD Biosciences, San Jose, CA) with 488 nm blue laser and phycoerythrin (PE) filters. Isolation of ALDHhi human hematopoietic stem cells Frozen human cord blood mononuclear cells were purchased from ALLCELLS (Alamenda, CA). Cells were thawed accordiFull Paper
2014Verma, R;Su, S;McCrann, DJ;Green, JM;Leu, K;Young, PR;Schatz, PJ;Silva, JC;Stokes, MP;Wojchowski, DM;RHEX, a novel regulator of human erythroid progenitor cell expansion and erythroblast developmentThe Journal of experimental medicine25092874O (and cytokine) challenges were at the concentrations and time intervals indicated. Primary human hematopoietic cells used in RT-PCR analyses included monocytes, neutrophils, T cells, and platelets (AllCells). Murine G1E-ER4 cells (M. Weiss, ChildreFull Paper
2014Roche, ET;Hastings, CL;Lewin, SA;Shvartsman, D;Brudno, Y;Vasilyev, NV;O'Brien, FJ;Walsh, CJ;Duffy, GP;Mooney, DJ;Comparison of biomaterial delivery vehicles for improving acute retention of stem cells in the infarcted heartBiomaterials24862441hMSCs (AllCells, USA) were encapsulated in chitosan/β-GP or alginate gels at a density of 100,000 cells/100μL gel (1 × 106 cells/mL) and 100μL was added to a hanging well cell culture insert with a pore size of 1μm, suitable for insertion into a 48-wFull Paper
2014Adamia, S;Haibe-Kains, B;Pilarski, PM;Bar-Natan, M;Pevzner, S;Avet-Loiseau, H;Lode, L;Verselis, S;Fox, EA;Burke, J;Galinsky, I;Dagogo-Jack, I;Wadleigh, M;Steensma, DP;Motyckova, G;Deangelo, DJ;Quackenbush, J;Stone, R;Griffin, JD;A genome-wide aberrant RNA splicing in patients with acute myeloid leukemia identifies novel potential disease markers and therapeutic targetsClinical cancer research : an official journal of the American Association for Cancer Research24284058A control group of 12 normal donors of both genders was also included: 8 bone marrow samples of normal donors were obtained from AllCells (California) and 4 peripheral blood SaveFull Paper
2014Wang, X;Moghimi, B;Zolotukhin, I;Morel, LM;Cao, O;Herzog, RW;Immune tolerance induction to factor IX through B cell gene transfer: TLR9 signaling delineates between tolerogenic and immunogenic B cellsMolecular therapy : the journal of the American Society of Gene Therapy24609143Primary B cells derived from healthy humans were purchased from Allcells (Alameda, CA). Cell culture reagents were purchased from Invitrogen (Grand Island, NY). Recombinant SaveFull Paper
2014Carbonaro, DA;Zhang, L;Jin, X;Montiel-Equihua, C;Geiger, S;Carmo, M;Cooper, A;Fairbanks, L;Kaufman, ML;Sebire, NJ;Hollis, RP;Blundell, MP;Senadheera, S;Fu, PY;Sahaghian, A;Chan, RY;Wang, X;Cornetta, K;Thrasher, AJ;Kohn, DB;Gaspar, HB;Preclinical demonstration of lentiviral vector-mediated correction of immunological and metabolic abnormalities in models of adenosine deaminase deficiencyMolecular therapy : the journal of the American Society of Gene Therapy24256635Normal human adult bone marrow samples (100 ml/donor) were also purchased from AllCells, LLC (Emeryville, CA). Human CD34+ cell isolation was performed as described.51 Briefly, human cord blood or human bone marrow was diluted 1:2 with Dulbecco's phoFull Paper
2014Huang, X;Shah, S;Wang, J;Ye, Z;Dowey, SN;Tsang, KM;Mendelsohn, LG;Kato, GJ;Kickler, TS;Cheng, L;Extensive ex vivo expansion of functional human erythroid precursors established from umbilical cord blood cells by defined factorsMolecular therapy : the journal of the American Society of Gene Therapy24002691Johns Hopkins University Institutional Review Board. Human primary CB MNCs are obtained from anonymous, healthy donors and then isolated in our lab using Ficoll-Paque Plus (P = 1.077) or directly purchased from AllCells, LLC (AllCells, Emeryville, CAFull Paper
2014Chorzalska, A;Salloum, I;Shafqat, H;Khan, S;Marjon, P;Treaba, D;Schorl, C;Morgan, J;Bryke, CR;Falanga, V;Zhao, TC;Reagan, J;Winer, E;Olszewski, AJ;Al-Homsi, AS;Kouttab, N;Dubielecka, PM;Low expression of Abelson interactor-1 is linked to acquired drug resistance in Bcr-Abl-induced leukemiaLeukemia24699303cross-talk may be causative in the acquired drug resistant phenotype of LSCs. Materials and methods Patients and donors Human CD34+ cells were isolated from bone marrow (BM) mononuclear cells (MNC) (AllCells, LLC, Emeryville, CA) or from peripheralFull Paper
2014Yang, H;Bueso-Ramos, C;DiNardo, C;Estecio, MR;Davanlou, M;Geng, QR;Fang, Z;Nguyen, M;Pierce, S;Wei, Y;Parmar, S;Cortes, J;Kantarjian, H;Garcia-Manero, G;Expression of PD-L1, PD-L2, PD-1 and CTLA4 in myelodysplastic syndromes is enhanced by treatment with hypomethylating agentsLeukemia24270737from bone marrow specimens using CD34+ MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s protocol. Bone marrow samples from 4 healthy donors were obtained from Allcells (Emeryville, CA). Peripheral blood (PB) samFull Paper
2014Kharabi Masouleh, B;Geng, H;Hurtz, C;Chan, LN;Logan, AC;Chang, MS;Huang, C;Swaminathan, S;Sun, H;Paietta, E;Melnick, AM;Koeffler, P;Müschen, M;Mechanistic rationale for targeting the unfolded protein response in pre-B acute lymphoblastic leukemiaProceedings of the National Academy of Sciences of the United States of America24821775For magnetic bead sorting, peripheral blood or bone marrow mononuclear cells were purchased from AllCells LLC and CD19-enrichment was performed by magnetic bead cell sorting SaveFull Paper
2014Murray, JC;Aldeghaither, D;Wang, S;Nasto, RE;Jablonski, SA;Tang, Y;Weiner, LM;c-Abl modulates tumor cell sensitivity to antibody-dependent cellular cytotoxicityCancer immunology research25300860Frozen primary peripheral blood mononuclear cells (PBMC) from three individual donors (AllCells) were enriched for NK cells (Human NK Cell Enrichment Kit; STEMCELL Technologies SaveFull Paper
2014Wang, Y;Yi, S;Sun, L;Huang, Y;Zhang, M;Charge-selective fractions of naturally occurring nanoparticles as bioactive nanocarriers for cancer therapyActa biomaterialia24952072Splenocytes, derived from C57BL/6 mice, were purchased from the Allcells Company (Emeryville, CA). HEPES, 1,9-dimethyl-methylene blue (DMMB), chondroitin sulfate (CS), Sephadex G75, DEAE-cellulose, and phosphate-buffered saline (PBS) were purchased fFull Paper
2014Fu, Y;Yip, A;Seah, PG;Blasco, F;Shi, PY;Hervé, M;Modulation of inflammation and pathology during dengue virus infection by p38 MAPK inhibitor SB203580Antiviral research25131378Dengue virus (DENV) infection could lead to dengue fever (DF), dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). The disease outcome is controlled by both viral SaveFull Paper
2014Rampal, R;Alkalin, A;Madzo, J;Vasanthakumar, A;Pronier, E;Patel, J;Li, Y;Ahn, J;Abdel-Wahab, O;Shih, A;Lu, C;Ward, PS;Tsai, JJ;Hricik, T;Tosello, V;Tallman, JE;Zhao, X;Daniels, D;Dai, Q;Ciminio, L;Aifantis, I;He, C;Fuks, F;Tallman, MS;Ferrando, A;Nimer, S;Paietta, E;Thompson, CB;Licht, JD;Mason, CE;Godley, LA;Melnick, A;Figueroa, ME;Levine, RL;DNA hydroxymethylation profiling reveals that WT1 mutations result in loss of TET2 function in acute myeloid leukemiaCell reports25482556Eleven human CD34 + bone marrow samples were provided by the Stem Cell and Xenograft Core Facility of the University of Pennsylvania or purchased from AllCells. These studies SaveFull Paper
2014Li, D;Xie, K;Ding, G;Li, J;Chen, K;Li, H;Qian, J;Jiang, C;Fang, J;Tumor resistance to anti-VEGF therapy through up-regulation of VEGF-C expressionCancer letters24333721Human umbilical vein endothelial cells (HUVECs) were obtained from Allcells (Emeryville, , and amphotericin-B (HUVEC-004; Allcells). All cells were authenticated according to the cell SaveFull Paper
2014Li, X;Tong, LJ;Ding, J;Meng, LH;Systematic combination screening reveals synergism between rapamycin and sunitinib against human lung cancerCancer letters24018642The human umbilical vein endothelial cells (HUVECs) were purchased from AllCells and maintained in M199 medium supplemented with 20% heat-inactivated FBS, penicillin (100 IU/ SaveFull Paper
2014Yao, WT;Wu, JF;Yu, GY;Wang, R;Wang, K;Li, LH;Chen, P;Jiang, YN;Cheng, H;Lee, HW;Yu, J;Qi, H;Yu, XJ;Wang, P;Chu, YW;Yang, M;Hua, ZC;Ying, HQ;Hoffman, RM;Jeong, LS;Jia, LJ;Suppression of tumor angiogenesis by targeting the protein neddylation pathwayCell death & disease24525735HUVECs were purchased from Allcells Biotech Co. (Shanghai, China) and cultured in a complete endothelial cell medium (Allcells Biotech Co.) at 37C with 5% CO 2 . SaveFull Paper
2014Li, D;Wei, X;Xie, K;Chen, K;Li, J;Fang, J;A novel decoy receptor fusion protein for FGF-2 potently inhibits tumour growthBritish journal of cancer24874473cells were purchased from AllCells (Emeryville, CA, USA) and cultured in complete HUVEC medium (AllCells) containing 10% FBS and 10% growth factor supplement (AllCells). Caki-1 SaveFull Paper
2014Haselmayer, P;Camps, M;Muzerelle, M;El Bawab, S;Waltzinger, C;Bruns, L;Abla, N;Polokoff, MA;Jond-Necand, C;Gaudet, M;Benoit, A;Bertschy Meier, D;Martin, C;Gretener, D;Lombardi, MS;Grenningloh, R;Ladel, C;Petersen, JS;Gaillard, P;Ji, H;Characterization of Novel PI3Kδ Inhibitors as Potential Therapeutics for SLE and Lupus Nephritis in Pre-Clinical StudiesFrontiers in immunology24904582terial smooth muscle cells and keratinocytes (Lonza, Inc., Allendale, NJ, USA) were as previously described. Positively selected primary normal human CD19+ B cells and CD4+ T-cells were obtained from AllCells (Emeryville, CA, USA). PBMC were isolatedFull Paper
2014Fu, J;Tjandra, M;Becker, C;Bednarczyk, D;Capparelli, M;Elling, R;Hanna, I;Fujimoto, R;Furegati, M;Karur, S;Kasprzyk, T;Knapp, M;Leung, K;Li, X;Lu, P;Mergo, W;Miault, C;Ng, S;Parker, D;Peng, Y;Roggo, S;Rivkin, A;Simmons, RL;Wang, M;Wiedmann, B;Weiss, AH;Xiao, L;Xie, L;Xu, W;Yifru, A;Yang, S;Zhou, B;Sweeney, ZK;Potent nonimmunosuppressive cyclophilin inhibitors with improved pharmaceutical properties and decreased transporter inhibitionJournal of medicinal chemistry25310383PBMCs of two human donors were obtained on the day of the experiment (AllCells, LLC), pelleted in their original 50 mL conical tubes at 1200 rpm for 10 min, and resuspended in 25 SaveFull Paper
2014Gandhi, AK;Mendy, D;Waldman, M;Chen, G;Rychak, E;Miller, K;Gaidarova, S;Ren, Y;Wang, M;Breider, M;Carmel, G;Mahmoudi, A;Jackson, P;Abbasian, M;Cathers, BE;Schafer, PH;Daniel, TO;Lopez-Girona, A;Thakurta, A;Chopra, R;Measuring cereblon as a biomarker of response or resistance to lenalidomide and pomalidomide requires use of standardized reagents and understanding of gene complexityBritish journal of haematology24206017here, H929 lenalidomide-resistant cells and DF15R pomalidomide-resistant cells were taken out of culture with compounds for 5-7 d before use. Select primary CD138+ MM cells were purchased from AllCells (Emeryville, CA, USA). Production of recombinantFull Paper
2014Sully, EK;Malachowa, N;Elmore, BO;Alexander, SM;Femling, JK;Gray, BM;DeLeo, FR;Otto, M;Cheung, AL;Edwards, BS;Sklar, LA;Horswill, AR;Hall, PR;Gresham, HD;Selective chemical inhibition of agr quorum sensing in Staphylococcus aureus promotes host defense with minimal impact on resistancePLoS pathogens24945495Human neutrophils were purchased from AllCells and the source of the neutrophils was Human neutrophils (AllCells) were washed twice in saline to remove EDTA, suspended in RPMI SaveFull Paper
2014Bellayr, IH;Catalano, JG;Lababidi, S;Yang, AX;Lo Surdo, JL;Bauer, SR;Puri, RK;Gene markers of cellular aging in human multipotent stromal cells in cultureStem cell research & therapy24780490Human MSCs from six different donors were purchased from ALLCELLS and Lonza (Table 1). Because these cells were commercially available, no patient consent or approval from the SaveFull Paper
2014Wei, DG;Chiang, V;Fyne, E;Balakrishnan, M;Barnes, T;Graupe, M;Hesselgesser, J;Irrinki, A;Murry, JP;Stepan, G;Stray, KM;Tsai, A;Yu, H;Spindler, J;Kearney, M;Spina, CA;McMahon, D;Lalezari, J;Sloan, D;Mellors, J;Geleziunas, R;Cihlar, T;Histone deacetylase inhibitor romidepsin induces HIV expression in CD4 T cells from patients on suppressive antiretroviral therapy at concentrations achieved by clinical dosingPLoS pathogens24722454ofectamine 2000 (Life Technologies, Grand Island, NY). In vitro model of HIV latency Total peripheral blood mononuclear cells (PBMCs) were obtained from healthy HIV-negative donors by leukapheresis (AllCells, Inc, Emeryville, CA). Naive CD4+ T cellsFull Paper
2014Jajosky, AN;Coad, JE;Vos, JA;Martin, KH;Senft, JR;Wenger, SL;Gibson, LF;RepSox slows decay of CD34+ acute myeloid leukemia cells and decreases T cell immunoglobulin mucin-3 expressionStem cells translational medicine24855276CD34 + cells isolated from the mobilized peripheral blood of healthy donors were purchased from AllCells (Emeryville, CA, http://www.allcells.com). SaveFull Paper
2014Ji, J;Judkowski, VA;Liu, G;Wang, H;Bunying, A;Li, Z;Xu, M;Bender, J;Pinilla, C;Yu, JS;Identification of novel human leukocyte antigen-A*0201-restricted, cytotoxic T lymphocyte epitopes on CD133 for cancer stem cell immunotherapyStem cells translational medicine24375541bone marrow cells were purchased directly from AllCells Inc. (Alameda, CA, http://www. ) cells (CD133+ and CD133‐ fractions; AllCells) from a healthy donor were included as controls. SaveFull Paper
2014Wang, X;Kimbrel, EA;Ijichi, K;Paul, D;Lazorchak, AS;Chu, J;Kouris, NA;Yavanian, GJ;Lu, SJ;Pachter, JS;Crocker, SJ;Lanza, R;Xu, RH;Human ESC-derived MSCs outperform bone marrow MSCs in the treatment of an EAE model of multiple sclerosisStem cell reports25068126BM-MSC lines #1, #2 and #3 were derived from frozen BM mononuclear cells (BMMNC) from 3 independent human donors and BM-MSC lines #7 and #8 were derived from fresh whole bone marrow aspirates from 2 independent donors from AllCells, Inc. BM-MSC lineFull Paper
2014Karjalainen, K;Pasqualini, R;Cortes, JE;Kornblau, SM;Lichtiger, B;O'Brien, S;Kantarjian, HM;Sidman, RL;Arap, W;Koivunen, E;Design, development, and validation of a high-throughput drug-screening assay for targeting of human leukemiaCancer24496871negative against blood-transmittable diseases. These samples were stored at 4°C for 24 hours before use. Heparin was used as an anticoagulant. Pretested whole blood and bone marrow samples also were obtained from commercial sources (Innovative ResearFull Paper
2014Polakowski, N;Terol, M;Hoang, K;Nash, I;Laverdure, S;Gazon, H;Belrose, G;Mesnard, JM;Césaire, R;Péloponèse, JM;Lemasson, I;HBZ stimulates brain-derived neurotrophic factor/TrkB autocrine/paracrine signaling to promote survival of human T-cell leukemia virus type 1-Infected T cellsJournal of virology25210182RNA from healthy donor CD4 T cells was purchased from AllCells. cDNA was synthesized Real-time PCR amplification of cDNAs from cell lines and cDNA purchased from AllCells was SaveFull Paper
2014Lucera, MB;Tilton, CA;Mao, H;Dobrowolski, C;Tabler, CO;Haqqani, AA;Karn, J;Tilton, JC;The histone deacetylase inhibitor vorinostat (SAHA) increases the susceptibility of uninfected CD4+ T cells to HIV by increasing the kinetics and efficiency of postentry viral eventsJournal of virology25008921CD4+ T cells were obtained by leukopheresis from ALLCELLS, LLC. All subjects were 151 Save;CD4 T cells were obtained by leukapheresis from Allcells, LLC. All subjects were healthy control donors negative for hepatitis B virus (HBV), hepatitis C virFull Paper
2014Tabler, CO;Lucera, MB;Haqqani, AA;McDonald, DJ;Migueles, SA;Connors, M;Tilton, JC;CD4+ memory stem cells are infected by HIV-1 in a manner regulated in part by SAMHD1 expressionJournal of virology24554663Samples from healthy donors were deidentified and were obtained by leukapheresis from AllCells, LLC. HIV-infected patients were recruited from the Clinical Research Center, NIH ( SaveFull Paper
2014Chen, YL;Abdul Ghafar, N;Karuna, R;Fu, Y;Lim, SP;Schul, W;Gu, F;Herve, M;Yokohama, F;Wang, G;Cerny, D;Fink, K;Blasco, F;Shi, PY;Activation of peripheral blood mononuclear cells by dengue virus infection depotentiates balapiravirJournal of virology24257621Antiviral assays. Cryopreserved human PBMCs were purchased from AllCells, LLC (Alameda, California). Written consent from the donors was available for all samples. All experiments involving human PBMCs were approved by the Institutional Review BoardFull Paper
2014Raval, AN;Schmuck, EG;Tefera, G;Leitzke, C;Ark, CV;Hei, D;Centanni, JM;de Silva, R;Koch, J;Chappell, RG;Hematti, P;Bilateral administration of autologous CD133+ cells in ambulatory patients with refractory critical limb ischemia: lessons learned from a pilot randomized, double-blind, placebo-controlled trialCytotherapy25239491Purity and viability studies were also performed on mobilized leukapheresis products obtained from healthy normal volunteers (AllCells, Almeda, CA, USA). The dose of G-CSF for SaveFull Paper
2014Hulspas, R;Villa-Komaroff, L;Koksal, E;Etienne, K;Rogers, P;Tuttle, M;Korsgren, O;Sharpe, JC;Berglund, D;Purification of regulatory T cells with the use of a fully enclosed high-speed microfluidic systemCytotherapy25065635kit (Stem Cell Technologies Inc, Vancouver, BC, Canada) from a total of 450 million fresh human peripheral blood mononucleated cells obtained from apheresis leuko paks (AllCells SaveFull Paper
2014Dasmahapatra, G;Patel, H;Friedberg, J;Quayle, SN;Jones, SS;Grant, S;In vitro and in vivo interactions between the HDAC6 inhibitor ricolinostat (ACY1215) and the irreversible proteasome inhibitor carfilzomib in non-Hodgkin lymphoma cellsMolecular cancer therapeutics25239935Interactions between the HDAC6 inhibitor ricolinostat (ACY1215) and the irreversible proteasome inhibitor carfilzomib were examined in non-Hodgkin lymphoma (NHL) models, SaveFull Paper
2014Feng, JY;Cheng, G;Perry, J;Barauskas, O;Xu, Y;Fenaux, M;Eng, S;Tirunagari, N;Peng, B;Yu, M;Tian, Y;Lee, YJ;Stepan, G;Lagpacan, LL;Jin, D;Hung, M;Ku, KS;Han, B;Kitrinos, K;Perron, M;Birkus, G;Wong, KA;Zhong, W;Kim, CU;Carey, A;Cho, A;Ray, AS;Inhibition of hepatitis C virus replication by GS-6620, a potent C-nucleoside monophosphate prodrugAntimicrobial agents and chemotherapy24419349Human primary bone marrow (BM) light-density cells from three different lots were obtained from AllCells (Emeryville, CA) or Lonza (Walkersville, MD), and the cytotoxicity studies were SaveFull Paper
2014Yao, H;Miura, Y;Yoshioka, S;Miura, M;Hayashi, Y;Tamura, A;Iwasa, M;Sato, A;Hishita, T;Higashi, Y;Kaneko, H;Ashihara, E;Ichinohe, T;Hirai, H;Maekawa, T;Parathyroid hormone enhances hematopoietic expansion via upregulation of cadherin-11 in bone marrow mesenchymal stromal cellsStem cells (Dayton, Ohio)24648356Parathyroid hormone (PTH) stimulates hematopoiesis in mouse models. The involvement of osteoblasts in this process has been well investigated; however, the effects of PTH on SaveFull Paper
2014Pandya, J;Ortiz, L;Ling, C;Rivers, AE;Aslanidi, G;Rationally designed capsid and transgene cassette of AAV6 vectors for dendritic cell-based cancer immunotherapyImmunology and cell biology24217810Leukapheresis-derived peripheral blood mononuclear cells (AllCells, Alameda, CA, USA) were purified on Ficoll-Paque (GE Healthcare, Piscataway, NJ, USA). Semi-adherent cell fractions were incubated in serum-free X-VIVO medium (Lonza, Allendale, NJ, UFull Paper
2014Yoshioka, S;Miura, Y;Yao, H;Satake, S;Hayashi, Y;Tamura, A;Hishita, T;Ichinohe, T;Hirai, H;Takaor-Kondo, A;Maekawa, T;CCAAT/enhancer-binding protein β expressed by bone marrow mesenchymal stromal cells regulates early B-cell lymphopoiesisStem cells (Dayton, Ohio)24115241The transcription factor CCAAT/enhancer-binding protein β (C/EBPβ) regulates the differentiation of a variety of cell types. Here, the role of C/EBPβ expressed by bone marrow SaveFull Paper
2014Brunckhorst, MK;Xu, Y;Lu, R;Yu, Q;Angiopoietins promote ovarian cancer progression by establishing a procancer microenvironmentThe American journal of pathology25043619Despite decades of research, the survival rate of ovarian cancer patients is largely unchanged. Current chemotherapeutic drugs are effective only transiently because patients with SaveFull Paper
2014Bowers, PM;Verdino, P;Wang, Z;da Silva Correia, J;Chhoa, M;Macondray, G;Do, M;Neben, TY;Horlick, RA;Stanfield, RL;Wilson, IA;King, DJ;Nucleotide insertions and deletions complement point mutations to massively expand the diversity created by somatic hypermutation of antibodiesThe Journal of biological chemistry25320089Sequencing and Preparation of PBMC cDNA RNA from peripheral blood lymphocytes (PBMCs) from a total of 68 healthy donors was purchased from two sources as follows: seven donors from AllCells, Inc. (Alameda, CA), and 61 donors from HemaCare Corp. (SanFull Paper
2014Edwards, W;Fung-Leung, WP;Huang, C;Chi, E;Wu, N;Liu, Y;Maher, MP;Bonesteel, R;Connor, J;Fellows, R;Garcia, E;Lee, J;Lu, L;Ngo, K;Scott, B;Zhou, H;Swanson, RV;Wickenden, AD;Targeting the ion channel Kv1.3 with scorpion venom peptides engineered for potency, selectivity, and half-lifeThe Journal of biological chemistry24939846Human PBMCs were obtained from AllCells LLC or purified from healthy donor blood by Ficoll-Paque (GE Healthcare) density centrifugation. Purified PBMCs were plated in 96-well SaveFull Paper
2014Stuible, M;Moraitis, A;Fortin, A;Saragosa, S;Kalbakji, A;Filion, M;Tremblay, GB;Mechanism and function of monoclonal antibodies targeting siglec-15 for therapeutic inhibition of osteoclastic bone resorptionThe Journal of biological chemistry24446437HOPs (CD14 + peripheral blood mononuclear cells) were isolated from normal human peripheral blood mononuclear cells (AllCells, Emeryville, CA) using CD14 microbeads and MS SaveFull Paper
2014Collins, E;Gu, F;Qi, M;Molano, I;Ruiz, P;Sun, L;Gilkeson, GS;Differential efficacy of human mesenchymal stem cells based on source of originJournal of immunology (Baltimore, Md. : 1950)25274529Human BM MSCs were derived from fresh whole BM of healthy donors (purchased from ALLCELLS) and four lupus patients. Lupus patients consisted of four African Americans (three SaveFull Paper
2014Lan, J;Yang, K;Byrd, D;Hu, N;Amet, T;Shepherd, N;Desai, M;Gao, J;Gupta, S;Sun, Y;Yu, Q;Provirus activation plus CD59 blockage triggers antibody-dependent complement-mediated lysis of latently HIV-1-infected cellsJournal of immunology (Baltimore, Md. : 1950)25149467Briefly, leukapheresis products from 3 HIV-1-negative blood donors were purchased from the ALLCells (Emeryville, CA) for isolation of primary CD4+ T cells using the negative selection RosetteSep reagents (StemCell Technologies, Vancouver, BC, Canada)Full Paper
2014Balan, S;Ollion, V;Colletti, N;Chelbi, R;Montanana-Sanchis, F;Liu, H;Vu Manh, TP;Sanchez, C;Savoret, J;Perrot, I;Doffin, AC;Fossum, E;Bechlian, D;Chabannon, C;Bogen, B;Asselin-Paturel, C;Shaw, M;Soos, T;Caux, C;Valladeau-Guilemond, J;Dalod, M;Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cellsJournal of immunology (Baltimore, Md. : 1950)25009205CD34 + cells from adult donor bone marrow or mobilized peripheral blood were purchased from properly licensed commercial companies (AllCells and StemCells). SaveFull Paper
2014Kajiwara, Y;Schiff, T;Voloudakis, G;Gama Sosa, MA;Elder, G;Bozdagi, O;Buxbaum, JD;A critical role for human caspase-4 in endotoxin sensitivityJournal of immunology (Baltimore, Md. : 1950)24879791Human monocyte-derived macrophages were purchased from AllCells (PB-MDM-001F; Alameda, CA) and cultured in RPMI 1640 supplemented with 10% FBS/penicillin/streptomycin and 10 ng/ml human M-CSF (R&D Systems). BMDM cultures were prepared according to prFull Paper
2014Kohn, LA;Seet, CS;Scholes, J;Codrea, F;Chan, R;Zaidi-Merchant, S;Zhu, Y;De Oliveira, S;Kapoor, N;Shah, A;Abdel-Azim, H;Kohn, DB;Crooks, GM;Human lymphoid development in the absence of common γ-chain receptor signalingJournal of immunology (Baltimore, Md. : 1950)24771849Normal human adult BM was obtained from healthy donors as anonymous waste materials isolated from filters after BM harvest and from commercial sources (AllCells, Alameda, CA). SaveFull Paper
2014Inage, E;Kasakura, K;Yashiro, T;Suzuki, R;Baba, Y;Nakano, N;Hara, M;Tanabe, A;Oboki, K;Matsumoto, K;Saito, H;Niyonsaba, F;Ohtsuka, Y;Ogawa, H;Okumura, K;Shimizu, T;Nishiyama, C;Critical Roles for PU.1, GATA1, and GATA2 in the expression of human FcεRI on mast cells: PU.1 and GATA1 transactivate FCER1A, and GATA2 transactivates FCER1A and MS4A2Journal of immunology (Baltimore, Md. : 1950)24639354Human primary mast cells were generated from peripheral blood CD34+ cells (Frozen Mobilized Peripheral Blood CD34+ cells, mPB015F; ALLCELLS, Emeryville, CA) as progenitors based on the previously established method (13). In three independent experimeFull Paper
2014Fukahori, H;Chida, N;Maeda, M;Tasaki, M;Kawashima, T;Matsuoka, H;Suzuki, K;Ishikawa, T;Tanaka, A;Higashi, Y;Effect of AS2521780, a novel PKCθ selective inhibitor, on T cell-mediated immunityEuropean journal of pharmacology25445039T cell-mediated immunity is central to the pathogenesis of autoimmune diseases, and is a target in the development of alternative therapeutic strategies with reduced adverse effects on SaveFull Paper
2014Kota, DJ;DiCarlo, B;Hetz, RA;Smith, P;Cox, CS;Olson, SD;Differential MSC activation leads to distinct mononuclear leukocyte binding mechanismsScientific reports24691433For Supplemental Figure 1, we also isolated MSCs from commercially available fresh bone marrow aspirates (AllCells, Emeryville, CA) according to the protocols previously established SaveFull Paper
2014Lee, JS;Kim, YR;Park, JM;Ha, SJ;Kim, YE;Baek, NI;Hong, EK;Mulberry fruit extract protects pancreatic β-cells against hydrogen peroxide-induced apoptosis via antioxidative activityMolecules (Basel, Switzerland)24972274and then 0.7 mM hydrogen peroxide was added to allcells for 4 h, with the exception of the h and then 0.7 mM hydrogen peroxide was added to allcells for 4 h, with the exception of the SaveFull Paper
2014Langevin, SM;Houseman, EA;Accomando, WP;Koestler, DC;Christensen, BC;Nelson, HH;Karagas, MR;Marsit, CJ;Wiencke, JK;Kelsey, KT;Leukocyte-adjusted epigenome-wide association studies of blood from solid tumor patientsEpigenetics24671036Briefly, sorted non-pathological human peripheral blood leukocytes (n = 47) were previously obtained from different anonymous individuals through AllCells, LLC. Leukocytes were SaveFull Paper
2014Xiao, W;Wang, J;Li, H;Xia, D;Yu, G;Yao, W;Yang, Y;Xiao, H;Lang, B;Ma, X;Guo, X;Guan, W;Xu, H;Liu, J;Zhang, X;Ye, Z;Fibulin-1 is epigenetically down-regulated and related with bladder cancer recurrenceBMC cancer25234557O2 maintained at 37°C. SV-HUC-1 was also purchased from ATCC and maintained in DMEM/F-12 medium. Primary human umbilical vein endothelial cell (HUVEC) and Endothelial Cell Medium were purchased from ALLCELLS, LLC (Shanghai, China). Plasmid transfectiFull Paper
2014Thorarensen, A;Banker, ME;Fensome, A;Telliez, JB;Juba, B;Vincent, F;Czerwinski, RM;Casimiro-Garcia, A;ATP-mediated kinome selectivity: the missing link in understanding the contribution of individual JAK Kinase isoforms to cellular signalingACS chemical biology24814050Cryopreserved human PBMCs (no. PB005F), which were used in the IL-2, IL-10, IL-15, and IFNα assays, were purchased from Allcells (Emeryville, CA). Frozen PBMCs were thawed in a water bath (37 °C) and washed once with once with 5% FBS/RPMI-1640Full Paper
2014Womble, TA;Green, S;Shahaduzzaman, M;Grieco, J;Sanberg, PR;Pennypacker, KR;Willing, AE;Monocytes are essential for the neuroprotective effect of human cord blood cells following middle cerebral artery occlusion in ratMolecular and cellular neurosciences24472845The cells (either HUCB or HPB MNC, obtained commercially from AllCells, LLC), were thawed rapidly at 37 C and transferred slowly into 10 ml of 10 mM PBS. The cells were then SaveFull Paper
2014Fraser, P;Macdonald, G;Mullineux, A;Regional Monetary Policy: An Australian PerspectiveRegional StudiesThis is a feature which has been under discussion and analysis for some time (for example, Tremosa-Ballcells and Pons-Novell, 2001; Mojon and Peersman, 2001; Aksoy et al., 2002; Clausen and Wohltmann, 2005; and more recently, Dow et al., 2012; and alFull Paper
2014Liu, X;Ou, YC;Zhang, J;Ahene, A;Clark, D;Hsieh, SC;Cooper, M;Ji, C;Pharmacokinetics and pharmacodynamics of CD4-anchoring bi-functional fusion inhibitor in monkeysPharmaceutical research24065594BD Biosciences (La Jolla, CA). Human PBMCs from healthy donors were purchased from AllCells (cat # PB001, Emeryville, CA) and cultured in complete medium (RPMI 1640, 10% FBS, 1% P/S, 1% L-Glutamine, 1% non-essential amino acid, 1% sodium pyruvate, 1%Full Paper
2014Wolstein, O;Boyd, M;Millington, M;Impey, H;Boyer, J;Howe, A;Delebecque, F;Cornetta, K;Rothe, M;Baum, C;Nicolson, T;Koldej, R;Zhang, J;Keech, N;Camba Colón, J;Breton, L;Bartlett, J;An, DS;Chen, IS;Burke, B;Symonds, GP;Preclinical safety and efficacy of an anti-HIV-1 lentiviral vector containing a short hairpin RNA to CCR5 and the C46 fusion inhibitorMolecular therapy. Methods & clinical development26015947ced overnight on RetroNectin (TaKaRa, Japan, #T100B) coated plates at various MOI, as indicated in the experimental methodology. CD34+ cells Primary human CD34+ HSPC were purchased commercially from AllCells; cells were directly isolated from granulFull Paper
2014Palasuk, J;Kamocki, K;Hippenmeyer, L;Platt, JA;Spolnik, KJ;Gregory, RL;Bottino, MC;Bimix antimicrobial scaffolds for regenerative endodonticsJournal of endodontics25201643Introduction Eliminating and/or inhibiting bacterial growth within the root canal system has been shown to play a key role in the regenerative outcome. The aim of this study was to SaveFull Paper
2014Zhang, HT;Wang, L;Ai, J;Chen, Y;He, CX;Ji, YC;Huang, M;Yang, JY;Zhang, A;Ding, J;Geng, MY;SOMG-833, a novel selective c-MET inhibitor, blocks c-MET-dependent neoplastic effects and exerts antitumor activityThe Journal of pharmacology and experimental therapeutics24741075HUVEC cells (passage 3) were first serum starved in complete medium (AllCells) for 24 hours and treated by SOMG-833 for 72 hours in media containing 3% bovine serum albumin and SaveFull Paper
2014Kimbrel, EA;Kouris, NA;Yavanian, GJ;Chu, J;Qin, Y;Chan, A;Singh, RP;McCurdy, D;Gordon, L;Levinson, RD;Lanza, R;Mesenchymal stem cell population derived from human pluripotent stem cells displays potent immunomodulatory and therapeutic propertiesStem cells and development24650034from fresh BM aspirates (AllCells LLC, Alameda, CA) using Histopaque 1077/Accuspin tubes (Sigma-Aldrich) or from frozen human BM mononuclear cells (AllCells LLC) from both male SaveFull Paper
2014Iwata, M;Sandstrom, RS;Delrow, JJ;Stamatoyannopoulos, JA;Torok-Storb, B;Functionally and phenotypically distinct subpopulations of marrow stromal cells are fibroblast in origin and induce different fates in peripheral blood monocytesStem cells and development24131213The cells were cultured in mesenchymal stromal cell (MSC) basal media with MSC stimulatory supplement according to the manufacturer's protocol (ALLCELLS), and then expanded in SaveFull Paper
2014Johnston, JM;Denning, G;Moot, R;Whitehead, D;Shields, J;Le Doux, JM;Doering, CB;Spencer, HT;High-throughput screening identifies compounds that enhance lentiviral transductionGene therapy25231175Fresh CD34+ cells were purchased from AllCells (Emeryville, CA, USA). Before arrival, CD34+ cells were mobilized from human bone marrow and isolated from the peripheral blood of three individual subjects. Flow cytometry was used to assess the purityFull Paper
2014Kamata, S;Miyagawa, S;Fukushima, S;Nakatani, S;Kawamoto, A;Saito, A;Harada, A;Shimizu, T;Daimon, T;Okano, T;Asahara, T;Sawa, Y;Improvement of cardiac stem cell sheet therapy for chronic ischemic injury by adding endothelial progenitor cell transplantation: analysis of layer-specific regional cardiac functionCell transplantation23562134Each CSC sheet was approximately 42 mm in diameter and 100 μm thick. Granulocyte colony-stimulating factor-mobilized EPCs of human origin (AllCells, MPB-017F; Emeryville, CA, USA) were labeled with 2 μM DiI-blue in vitro (Molecular Probes) (33). TheFull Paper
2014Xiao, H;Li, H;Yu, G;Xiao, W;Hu, J;Tang, K;Zeng, J;He, W;Zeng, G;Ye, Z;Xu, H;MicroRNA-10b promotes migration and invasion through KLF4 and HOXD10 in human bladder cancerOncology reports24573354SV-HUC-1 was also purchased from ATCC and maintained in DMEM/F-12 medium. SCaBER was purchased from AllCells, LLC (Shanghai, China) in MEM medium. Paired samples of primary BC, and lymph node metastatic tissues were obtained from patients who had undFull Paper
2014Czubak, P;Bojarska-Junak, A;Tabarkiewicz, J;Putowski, L;A modified method of insulin producing cells' generation from bone marrow-derived mesenchymal stem cellsJournal of diabetes research25405207Human bone marrow stromal cells (MSCs) were purchased from a commercial source (AllCells; catalog number MSC-001F). This cell line was derived from one, nondiabetic Caucasian SaveFull Paper
2014Gupta, MS;Nicoll, SB;Functional nucleus pulposus-like matrix assembly by human mesenchymal stromal cells is directed by macromer concentration in photocrosslinked carboxymethylcellulose hydrogelsCell and tissue research25092545Human MSCs were purified from bone marrow (AllCells, Emeryville, CA, USA) using enriched density centrifugation as previously described (Gupta et al. 2011; Chen et al. 2013). Briefly, a Human MSC Enrichment Cocktail (StemCell Technologies, Vancouver,Full Paper
2014Pavlides, S;Gutierrez-Pajares, JL;Iturrieta, J;Lisanti, MP;Frank, PG;Endothelial caveolin-1 plays a major role in the development of atherosclerosisCell and tissue research24390341Human umbilical vein endothelial cells (HUVEC) were obtained from AllCells (AllCells, LLC, Emeryville, CA, USA). HUVEC were cultured in M199 media (Life Technologies, Grand Island, NY, USA) containing 10 % FBS (Life Technologies, Grand Island, NY, USFull Paper
2014Kiang, JG;Garrison, BR;Smith, JT;Fukumoto, R;Ciprofloxacin as a potential radio-sensitizer to tumor cells and a radio-protectant for normal cells: differential effects on γ-H2AX formation, p53 phosphorylation, Bcl-2 production, and cell deathMolecular and cellular biochemistry24802382Human B lymphoblastoid cell line TK6 (p53+/+) and human NH32 (p53−/− of TK6 cells) were generous gifts from Dr. James Mitchell. Human peripheral blood mononuclear cells (PBMCs) were purchased from AllCells (Emeryville, CA). Cells were grown in RPMI 1Full Paper
2014Dong, B;Zhou, H;Han, C;Yao, J;Xu, L;Zhang, M;Fu, Y;Xia, Q;Ischemia/reperfusion-induced CHOP expression promotes apoptosis and impairs renal function recovery: the role of acidosis and GPR4PloS one25343248Human umbilical vein endothelial cells (HUVECs), as well as supplier's recommended medium and supplement were purchased from ALLCELLS (Emeryville, California, USA). Human SaveFull Paper
2014Iwata, M;Torok-Storb, B;Wayner, EA;Carter, WG;CDCP1 identifies a CD146 negative subset of marrow fibroblasts involved with cytokine productionPloS one25275584The cells were cultured in MSC Basal media with MSC stimulatory supplement according to the manufacturer's protocol (ALLCELLS, Emeryville, CA). The cells were passaged until the SaveFull Paper
2014Ayala-Lujan, JL;Vijayakumar, V;Gong, M;Smith, R;Santiago, AE;Ruiz-Perez, F;Broad spectrum activity of a lectin-like bacterial serine protease family on human leukocytesPloS one25251283Fresh human blood to isolate primary cells were purchased from Allcells LLC. (Alameda, Fresh heparinized blood from healthy human donors purchased from AllCells LLC. (Alameda, SaveFull Paper
2014Kang, Y;Hodges, A;Ong, E;Roberts, W;Piermarocchi, C;Paternostro, G;Identification of drug combinations containing imatinib for treatment of BCR-ABL+ leukemiasPloS one25029499, USA) supplemented with 10% fetal bovine serum (FBS, Hyclone, USA), 100 U/ml penicillin, 100 µg/ml streptomycin (Hyclone, USA) at 37°C in 5% CO2. CD34+ hematopoietic stem cells were purchased from Allcells and maintained in StemSpan SFEM (STEMCELL TFull Paper
2014Lewis, ND;Patnaude, LA;Pelletier, J;Souza, DJ;Lukas, SM;King, FJ;Hill, JD;Stefanopoulos, DE;Ryan, K;Desai, S;Skow, D;Kauschke, SG;Broermann, A;Kuzmich, D;Harcken, C;Hickey, ER;Modis, LK;A GPBAR1 (TGR5) small molecule agonist shows specific inhibitory effects on myeloid cell activation in vitro and reduces experimental autoimmune encephalitis (EAE) in vivoPloS one24967665Human peripheral blood mononuclear cells isolated with leukapheresis were purchased from AllCells (Alameda, CA), frozen in 80% FBS, 10% RPMI and 10% DMSO and stored in SaveFull Paper
2014Simeonov, KP;Uppal, H;Direct reprogramming of human fibroblasts to hepatocyte-like cells by synthetic modified mRNAsPloS one24963715ield of direct reprogramming can overcome its retroviral reliance and be translated to its ultimate applications in regenerative medicine. Materials and Methods Cell Culture CD34+ bone marrow cells (AllCells, Emeryville, CA) were received fresh andFull Paper
2014Hong, KU;Guo, Y;Li, QH;Cao, P;Al-Maqtari, T;Vajravelu, BN;Du, J;Book, MJ;Zhu, X;Nong, Y;Bhatnagar, A;Bolli, R;c-kit+ Cardiac stem cells alleviate post-myocardial infarction left ventricular dysfunction despite poor engraftment and negligible retention in the recipient heartPloS one24806457m (A LV) which included the infarcted and border zones, posterior LV wall (nonischemic region; P LV), and atria+right ventricle. Female human peripheral mononuclear cells (hPBMCs) were purchased from AllCells, LLC (Emeryville, CA). Aliquots of 105 hPFull Paper
2014Gañán-Gómez, I;Wei, Y;Yang, H;Pierce, S;Bueso-Ramos, C;Calin, G;Boyano-Adánez, Mdel C;García-Manero, G;Overexpression of miR-125a in myelodysplastic syndrome CD34+ cells modulates NF-κB activation and enhances erythroid differentiation arrestPloS one24690917Human bone marrow cells from 6 healthy individuals were obtained from AllCells (Emeryville, CA). Isolation of CD34 + cells was performed using MicroBead Kit (Miltenyi, Bergisch SaveFull Paper
2014Chyu, KY;Lio, WM;Dimayuga, PC;Zhou, J;Zhao, X;Yano, J;Trinidad, P;Honjo, T;Cercek, B;Shah, PK;Cholesterol lowering modulates T cell function in vivo and in vitroPloS one24647529ential batch-to-batch differences in the biochemical contents in the FBS which may affect the experimental outcomes. The same experiment was performed using human peripheral blood mononuclear cells (AllCells, Emeryville, CA). T cells were isolated fFull Paper
2014Chen, J;Li, C;Wang, S;Periodic heat shock accelerated the chondrogenic differentiation of human mesenchymal stem cells in pellet culturePloS one24632670nd Methods All reagents and chemicals without manufacture labels were purchased from Sigma-Aldrich (St Louis, MO). hMSC Isolation and Characterization Human MSCs were isolated from bone marrow (BM) (AllCells LLC, Berkeley, CA) as described previouslFull Paper
2014Wang, J;Guo, X;Lui, M;Chu, PJ;Yoo, J;Chang, M;Yen, Y;Identification of a distinct small cell population from human bone marrow reveals its multipotency in vivo and in vitroPloS one24465489This study was conducted using data obtained from 70 fresh hPB and 30 fresh hBM samples purchased from AllCells, LLC. A list of the reagents and antibodies used in this study is SaveFull Paper
2014Beckwith, R;Worden, E;Estrin, E;Martin, A;Reconstitution of the 26S Proteasome Reveals Functional Asymmetries in its Heterohexameric AAA+ UnfoldaseBiophysical JournalProteins are complex and versatile macromolecules that are integral components of allcells. Among other things, proteins are responsible for structural organization, signalingand SaveFull Paper
2014Aikawa, N;Kunisato, A;Nagao, K;Kusaka, H;Takaba, K;Ohgami, K;Detection of thalidomide embryotoxicity by in vitro embryotoxicity testing based on human iPS cellsJournal of pharmacological sciences24451995The hiPSCs were derived from human bone marrow mononuclear cells which were purchased from AllCells (Emeryville, CA, USA) (11). Human dermal fibroblasts isolated from adult SaveFull Paper
2014Chen, JK;Li, ZP;Liu, YZ;Zhao, T;Zhao, XB;Ni, M;Jiang, GJ;Shen, FM;Activation of alpha 7 nicotinic acetylcholine receptor protects mice from radiation-induced intestinal injury and mortalityRadiation research24828112Radiation-induced gastrointestinal syndrome occurs when the body is exposed to a high dose of radiation. Currently, safe and effective radioprotectants are not available. Apoptosis SaveFull Paper
2014Lee, M;Jeong, SY;Ha, J;Kim, M;Jin, HJ;Kwon, SJ;Chang, JW;Choi, SJ;Oh, W;Yang, YS;Kim, JS;Jeon, HB;Low immunogenicity of allogeneic human umbilical cord blood-derived mesenchymal stem cells in vitro and in vivoBiochemical and biophysical research communications24657442Abstract Evaluation of the immunogenicity of human mesenchymal stem cells (MSCs) in an allogeneic setting during therapy has been hampered by lack of suitable models due to SaveFull Paper
2014Shima, T;Miyamoto, T;Kikushige, Y;Yuda, J;Tochigi, T;Yoshimoto, G;Kato, K;Takenaka, K;Iwasaki, H;Mizuno, S;Goto, N;Akashi, K;The ordered acquisition of Class II and Class I mutations directs formation of human t(8;21) acute myelogenous leukemia stem cellExperimental hematology25101977Human marrow was purchased from AllCells (Emeryville, CA). Informed consent was obtained from all patients. The Institutional Review Board of Kyushu University Hospital (Fukuoka, SaveFull Paper
2014Iancu-Rubin, C;Mosoyan, G;Glenn, K;Gordon, RE;Nichols, GL;Hoffman, R;Activation of p53 by the MDM2 inhibitor RG7112 impairs thrombopoiesisExperimental hematology24309210Human MK were generated in a two-step liquid culture system from bone marrow (BM)-derived CD34+ cells (AllCells, Emeryville, CA, USA). Cells were suspended in IMDM-based MK differentiation medium [6] for 7 days then allowed to mature for 7-9 additionFull Paper
2014Kornblau, SM;Cohen, AC;Soper, D;Huang, YW;Cesano, A;Age-related changes of healthy bone marrow cell signaling in response to growth factors provide insight into low risk MDSCytometry. Part B, Clinical cytometry24106013patients aged 54 to 82 years undergoing planned orthopedic procedures at Williamson Medical Center in Tennessee. The second (“younger”) healthy sample set consisted of 6 cryopreserved BMMC obtained from donors aged 23 to 43 years, purchased from a coFull Paper
2014Barrionuevo, M;Society of Toxicology (SOT) 5st Annual Meeting and ToxExpo, San Francisco, CA, USA, March 11-15, 2012Drugs of the Future283 on human myeloid, erythroid and mesenchymal lineages for its potential to protect marrow progenitor cells from radiation damage was discussed by Emer Clarke from ReachBio. SaveFull Paper
2014Fu, Y;Chen, YL;Herve, M;Gu, F;Shi, PY;Blasco, F;Development of a FACS-based assay for evaluating antiviral potency of compound in dengue infected peripheral blood mononuclear cellsJournal of virological methods24140514Isolated cryopreserved human PBMCs were obtained from AllCells, LLC. The PBMCs were obtained with informed consent and experiments involving PBMCs were approved by SaveFull Paper
2014Tilton, CA;Tabler, CO;Lucera, MB;Marek, SL;Haqqani, AA;Tilton, JC;A combination HIV reporter virus system for measuring post-entry event efficiency and viral outcome in primary CD4+ T cell subsetsJournal of virological methods24025341Peripheral blood mononuclear cells (PBMCs) and CD4+ T cells were obtained from leukopheresis from ALLCELLS, LLC on five healthy donors. All subjects for this study were de- SaveFull Paper
2014Shiheido, H;Kitagori, K;Sasaki, C;Kobayashi, S;Aoyama, T;Urata, K;Oku, T;Hirayama, Y;Yoshitomi, H;Hikida, M;Yoshifuji, H;Mimori, T;Watanabe, T;Shimizu, J;Human T cells expressing BEND3 on their surface represent a novel subpopulation that preferentially produces IL-6 and IL-8Immunity, inflammation and disease25400923, #16-15 Abs, and 7AAD, and BEND3+ or BEND3− subpopulations in CD4+ or CD8+ T cells were sorted using a flow cytometer (FACSAria, BD Biosciences, San Jose, CA). Cord blood cells were purchased from ALLCELLS, and Jurkat [25] cells were from the AmericFull Paper
2014Cheng, ZJ;Garvin, D;Paguio, A;Moravec, R;Engel, L;Fan, F;Surowy, T;Development of a robust reporter-based ADCC assay with frozen, thaw-and-use cells to measure Fc effector function of therapeutic antibodiesJournal of immunological methods25086226PBMCs from different donors were from AllCells. PNGase F, Magne™ Protein G Beads, Bio-Glo™ Luciferase Assay System and CytoTox 96® Non-Radioactive Cytotoxicity Assay were from PromegaFull Paper
2014Furuya, T;Sá, JM;Chitnis, CE;Wellems, TE;Stedman, TT;Reticulocytes from cryopreserved erythroblasts support Plasmodium vivax infection in vitroParasitology international24291603Plasmodium vivax is the most widely distributed human malaria parasite. Despite its importance, both clinical research and basic research have been hampered by lack of a convenient SaveFull Paper
2014Meissner, TB;Mandal, PK;Ferreira, LM;Rossi, DJ;Cowan, CA;Genome editing for human gene therapyMethods in enzymology25398345CD34 + cells from G-CSF mobilized peripheral blood were purchased from AllCells. CD34 + cells were cotransfected with CRISPR/Cas9 expression plasmids using Amaxa SaveFull Paper
2014Wilson, EM;Bial, J;Tarlow, B;Bial, G;Jensen, B;Greiner, DL;Brehm, MA;Grompe, M;Extensive double humanization of both liver and hematopoiesis in FRGN miceStem cell research25310256rich Chemicals; non-platable human hepatocytes (> 70% viable by trypan blue staining) were purchased from BioreclamationIVT (catalog# F00995); human frozen bone marrow CD34+ cells were purchased from AllCells (Catalog# ABM017F); HCM media was purchasFull Paper
2014Yourek, G;Xin, X;Reilly, GC;Mao, JJ;Infiltration of mesenchymal stem cells into PEGDA hydrogelBio-medical materials and engineering25201394Human MSCs were isolated from commercially available whole bone marrow (AllCells, Berkeley, CA) using "RosetteSep " (StemCell Technologies; Vancouver, BC) according to the SaveFull Paper
2014Chang, TP;Vancurova, I;Bcl3 regulates pro-survival and pro-inflammatory gene expression in cutaneous T-cell lymphomaBiochimica et biophysica acta25089799The advanced stages of cutaneous T cell lymphoma (CTCL) are characterized not only by decreased levels of pro-inflammatory cytokines, resulting in high susceptibility to infections, SaveFull Paper
2014Li, J;Sun, W;Subrahmanyam, PB;Page, C;Younger, KM;Tiper, IV;Frieman, M;Kimball, AS;Webb, TJ;NKT Cell Responses to B Cell LymphomaMedical sciences (Basel, Switzerland)24955247tained from commercial vendors. Specifically, buffy coats were purchased from Biological Specialty Corporation and peripheral blood from two different, newly diagnosed MCL patients was purchased from AllCells, LLC (Alameda, CA, USA). PBMCs were isolaFull Paper
2014Gitschier, HJ;Bergeron, AB;Randle, DH;Label-free cell-based dynamic mass redistribution assaysCurrent protocols in chemical biology24652622Human umbilical vein endothelial cells (Corning, cat. no. 354151) Peripheral blood mononuclear cells (AllCells, cat. no. PB006F) Appropriate cell culture/differentiation medium with supplements, e.g.: IMDM, KnockOut DMEM/F-12 with Stempro supplement,Full Paper
2014Vij, R;Mazumder, A;Klinger, M;O'Dea, D;Paasch, J;Martin, T;Weng, L;Park, J;Fiala, M;Faham, M;Wolf, J;Deep sequencing reveals myeloma cells in peripheral blood in majority of multiple myeloma patientsClinical lymphoma, myeloma & leukemia24629890The remaining 13 paired bone marrow and peripheral blood samples were purchased from a commercial source (AllCells, Emeryville, CA). SaveFull Paper
2014DeClerck, Y;Environment-Mediated Drug Resistance in NeuroblastomaU.S. Army Medical Research Material CommandHuman BMMSC were purchased from AllCells LLC. Monocytes of normal healthy donors were obtained from peripheral blood and separated by Ficoll density gradient centrifugation SaveFull Paper
2014Kornblau, S;The Use of “Omics” to Guide the Selection of Targeted TherapyTargeted Therapy of Acute Myeloid LeukemiaIf the normal HSC frequency is 1:100,000, then multiply this by 10. For our protein work, we have therefore used a CD34+ cell from a normal bone marrow as our standard comparator (we have successfully used CD34+ cells from AllCells for our RPPA work)Full Paper
2014허운학, ;Concurrent treatment with capecitabine and radiosurgery in the mouse glioblastoma modelThesisAllCells were cultured for two to three passages in Dulbecco's modified Eagle's medium (WelGENE, Daegu, Korea) containing 10% fetal bovine serum and 1% antibiotic-antimycotic SaveFull Paper
2014Zaidi-Merchant, SS;The role of let-7c/miR-125b/miR-99a in the hematopoietic development in the human bone marrowThesisNormal human bone marrow samples were obtained from healthy donors via the UCLA Pathology Tissue Core, Cincinnati Children’s Hospital, or ALLCELLS according to guidelines approved by UCLA Institutional Review Board. Fresh BM samples were Ficoll-PaqueFull Paper
2014Holm, HH;A CUDA Back-End for the Equelle Compiler.ThesisThe function Gradient takes a Collection Of Scalar On AllCells() as input and compute the discrete If u is a variable defined as Collection Of Scalar On AllCells() then the following line is SaveFull Paper
2014Oien, N;Erythrocyte Carriers: Tool Development for Erythrocyte Mediated Diagnostic and Drug DeliveryThesisPeripheral erythrocytes were purchased from AllCells. Dialysis cassettes were purchased Peripheral red blood cells were purchased from AllCells. 8-(4-chlorophenylthio) adenosine-3′ SaveFull Paper
2014Nguyen, L;LONG WAVELENGTH FLUORESCENT REPORTERS FOR PROTEIN KINASES AND DESIGN OF LIGHT-ACTIVATABLE BIOAGENTSThesisH89 and KT5720 were purchased from Sigma-Aldrich and Calbiochem (EMD), respectively. Peripheral red blood cells were purchased from AllCells. 8-(4-chlorophenylthio) adenosine-3′,5′-cyclic monophosphorothioate (CPT-cAMP), ATP, and cAMP were purchasedFull Paper
2014Rathbone, E;Inhibitory and Immunomodulatory Properties of Human Choroid Plexus Epithelial CellsThesisPooled BMSC, ADSC (both Lonza, Basel, Switzerland), DPSC (AllCells L.L.C., Alameda, CA, USA) and AMSC were cultured in high glucose Dulbecco’s Modified Eagle Medium (HG-DMEM; Biosera, Boussens, France) supplemented with 10% foetal bovine serum (FBS),Full Paper
2014Suganyaa, MV;Deepa, MKRS;Vinothini, MG;Operating the SRAM Cell Architecture with the High Efficient and Reduced Memory Sizeijaist.comIt has a multiplicative effect; allcells in the array are drawing this power. The CMOS 8T cellrequires about 60 times more static power than SRAM cells inthis study. The SRAM 8T cell SaveFull Paper
2014Yu, Y;Energy prediction of electric floor radiation systems using a new integrated modeling approachASHRAE TransactionsIn this figure, PMV Onezone and PPD Onezone represent the PMV and PPD values when the one-zone (multi-zone) model is applied and PMV Allcells and PPD Allcells represent the SaveFull Paper
2014Xiao, F;Genomic study of familial breast cancer-genomic instability, classification and predispositionThesisThe cells for the study were purchased from AllCells LLC (http://www.allcells.com/, Emeryville, California), which supplies human blood cells from donors for research and the company has its full IRB system (Biomed IRB). The signed written consent foFull Paper
2014Perignon, MC;A Rolling Stone Gathers No Moss: Modeling the Geomorphic Impacts of Floods in the Southern Rocky Mountain Region, USAThesis(Top, right) Non-local survival function for sediment entering the system at the mouth of the fan, for allcells with ow depth greater than 1 cm. (Bottom, right) Non-local probability of SaveFull Paper
2014Suwannatat, P;A Visual Analysis Toolkit for Microscopic Image Mosaics of Retinal AstrocytesThesis5 The cell locations are kept at allcells.txt under the data directory. as a gray cell among multi-hue neighbors with 100% saturations. with a "new cell here" command of the mouse SaveFull Paper
2014Johnson, SM;Extracellular matrix improves mesenchymal stem cell growth and maintenance of stemness in serum free mediumThesisFreshly isolated human bone marrow mononuclear cells, containing BM-MSCs (primary cells) from 20-25 year old donors were purchased from ALLCELLS (Emeryville, CA). Greater SaveFull Paper
2014Tsai, HC;Molecular Mechanism in the Regulation of Human Th17 Cell Differentiation and FunctionThesisThe use of human cells in the study was periodically reviewed and approved by the Human Subject Research Review Committee (University of California, Davis). PBMCs were purchased from Allcells. LLC. Samples used in a single experiment were from differFull Paper
2014Spinler, KR;Membrane forces and key protein determinants of hematopoietic cell function: Lamins and myosin-II in hematopoiesis and CD47 in immunotherapy of cancerThesisFresh purified bone marrow-derived human CD34+ cells and total mononuclear cells were obtained from either the Penn Xenograft Core Facility or AllCells (Emeryville, CA). Cells from at least five different donors were used in this study. Purity of thFull Paper
2014Amir, ED;viSNE and Wanderlust, two algorithms for the visualization and analysis of high-dimensional single-cell dataThesisFresh, Ficoll-enriched human bone marrow was obtained from healthy donors from AllCells, Inc. (Emeryville, CA). Samples were obtained with informed consent in accordance with the Declaration of Helsinki and with accordance with Stanford University’sFull Paper
2014Qin, S;The use of biomaterials for cell function enhancement: acceleration of fibroblast migration and promotion of stem cell proliferationThesisHuman cord blood CD34+ cells were purchased from AllCells LLC and cultured in StemSpan SFEM media, 10% FBS, 100 ng/ml each of human SCF, TPO, and Flt-3L. Cells were infected with ICD-Notch or GFP control lentiviral particles then plated on hydrogelsFull Paper
2014Krzeszinski, JY;Wei, W;Huynh, H;Jin, Z;Wang, X;Chang, TC;Xie, XJ;He, L;Mangala, LS;Lopez-Berestein, G;Sood, AK;Mendell, JT;Wan, Y;miR-34a blocks osteoporosis and bone metastasis by inhibiting osteoclastogenesis and Tgif2Nature25043055Human PBMN cells (ReachBio) were differentiated into osteoclasts in α-MEM containing 10% FBS, 25 ng m1 −l MCSF, 50 ng m1 −l hRANKL, 1 μM Dexamethasome and 1 μM Save;odeoxyuridine (BrdU) cell proliferation assay kit (GE Healthcare) as described27. OFull Paper
2014Shah, N;Cortes, J;Martinelli, G;Smith, B;Clarke, E;Copland, M;Strauss, L;Talpaz, M;Dasatinib Plus Smoothened (SMO) Inhibitor BMS-833923 in Chronic Myeloid Leukemia (CML) with Resistance or Suboptimal Response to a Prior Tyrosine Kinase Inhibitor (TKI): Phase I Study CA180323BloodUniversity of California, San Francisco, San Francisco, CA++The University of Texas MD Anderson Cancer Center, Houston, TX++University of Bologna, Bologna, Italy++Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD++ReachBio LLCFull Paper
2014Chattopadhyay, R;Dyukova, E;Singh, NK;Ohba, M;Mobley, JA;Rao, GN;Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C ε-mediated zona occludens-1 phosphorylation at threonine 770/772The Journal of biological chemistry24338688The human peripheral blood CD14+ monocytes were purchased from ReachBio LLC (Seattle, WA), suspended in RPMI 1640 medium containing 50 μm 2-mercaptoethanol, 100 IU/ml penicillin, and 100 μg/ml streptomycin, and used for transmigration assay. To colleFull Paper
2014Winter, JM;Jacobson, P;Bullough, B;Christensen, AP;Boyer, M;Reems, JA;Long-term effects of cryopreservation on clinically prepared hematopoietic progenitor cell productsCytotherapy24910385CFC assays were performed 21, 22 with either commercially available MethoCult (StemCell Technologies Inc, Vancouver, Canada) or ColonyGel (ReachBio, LLC, Seattle, WA, USA) that had been internally validated to show comparable results. Briefly, HPC(A)Full Paper
2014Chandrasekaran, D;Nakamoto, B;Watts, KL;Kiem, HP;Papayannopoulou, T;Modeling promising nonmyeloablative conditioning regimens in nonhuman primatesHuman gene therapy24937231Transduced, mock-transduced, and BM white blood cell samples from transplanted macaques were cultured in semisolid methylcellulose medium for 12-14 days in the presence of rhIL-3, recombinant human erythropoietin (rhEPO), rhSCF, and recombinant humanFull Paper
2014Cook, GJ;Caudell, DL;Elford, HL;Pardee, TS;The efficacy of the ribonucleotide reductase inhibitor Didox in preclinical models of AMLPloS one25402485bated as indicated above for 24 hours with a titration of Didox. Cells were washed with PBS and resuspended in IMDM supplemented with 20% FBS, and placed in ColonyGel High Cytokine Formulation media (ReachBio). Experiments were performed in triplicatFull Paper
2014Byon, JC;Padilla, SM;Papayannopoulou, T;Deletion of Dicer in late erythroid cells results in impaired stress erythropoiesis in miceExperimental hematology24971698Colony Forming Assay Peripheral blood, spleen and bone marrow mononuclear cells were plated in methylcellulose containing hematopoietic growth factors (ReachBio, WA) as previously described [17]Full Paper
2014Li, X;Xu, X;Ji, T;Liu, Z;Gu, M;Hoang, BH;Zi, X;Dietary feeding of Flavokawain A, a Kava chalcone, exhibits a satisfactory safety profile and its association with enhancement of phase II enzymes in miceToxicology reports25379458ated, the yield and viability of cells were determined by Trypan blue exclusion method. A total number of 2 × 104 cells were seeded in a 6-well plate containing Colony Gel 1201 Mouse Base Medium (Reachbio), and then treated with FKA and Adriamycin aFull Paper
2014Erythroid, IVEOS;Progenitor Populations And Analogous Human Stress Erythroid ProgenitorsThesisHuman bone marrow mononuclear cells were purchased from Reachbio [26-‐200] and cultured in the stress erythropoiesis medium described above. On the indicated days, cells were SaveFull Paper
2014Smuda, CA;The Transcriptional Regulator Ikaros and Control of Mast Cell and Basophil DevelopmentThesisWe took advantage of the sorting strategies developed for quantitation of progenitor subtypes to isolate these populations for further analysis in solid culture media. IL-3 (5 ng/mL) and SCF (12.5 ng/mL) was added ColonyGel 1201 base methylcelluloseFull Paper
2013Singh, R;Low, ET;Ooi, LC;Ong-Abdullah, M;Ting, NC;Nagappan, J;Nookiah, R;Amiruddin, MD;Rosli, R;Manaf, MA;Chan, KL;Halim, MA;Azizi, N;Lakey, N;Smith, SW;Budiman, MA;Hogan, M;Bacher, B;Van Brunt, A;Wang, C;Ordway, JM;Sambanthamurthi, R;Martienssen, RA;The oil palm SHELL gene controls oil yield and encodes a homologue of SEEDSTICKNature23883930We thank Johannes Jansen (PRI, Wageningen, Netherlands) for assistance with JoinMap, and Beijing Genome Institute, The Genome Institute at Washington University, Tufts University Core Facility and the Arizona Genome Institute for mapping and genome sFull Paper
2013Ong, CT;Van Bortle, K;Ramos, E;Corces, VG;Poly(ADP-ribosyl)ation regulates insulator function and intrachromosomal interactions in DrosophilaCell24055367We would like to thank the Genomic Services Lab at the HudsonAlpha Institute for Biotechnology for their help in performing Illumina sequencing of ChIP-Seq samples. This work was supported by U.S. Public Health Service Award R01 GM035463 from the NatFull Paper
2013Mukhopadhyay, S;Wen, X;Ratti, N;Loktev, A;Rangell, L;Scales, SJ;Jackson, PK;The ciliary G-protein-coupled receptor Gpr161 negatively regulates the Sonic hedgehog pathway via cAMP signalingCell23332756For the ISH experiments using radiolabeled probes, custom service from Phylogeny Inc. (Columbus, OH) was used. Both antisense and sense radiolabeled riboprobes were synthesized in vitro according to the manufacturer’s specifications (Ambion) and labeFull Paper
2013Locke, D;Bernstein, S;Lynch, F;Siami-Namini, K;Walling, J;Yonker, T;Yarranton, G;An IHC Screen For EphA3 Positive FFPE TumorsBloodNovember 15, 2013. An IHC Screen For EphA3 Positive FFPE Tumors. Darren Locke, PhD Darren Locke, PhD. 1. QualTek Molecular Laboratories, Newtown, USA,. 1. Search for other works by this author on: This Site. PubMed. GoogleFull Paper
2013Yang, J;Sung, E;Donlin-Asp, PG;Corces, VG;A subset of Drosophila Myc sites remain associated with mitotic chromosomes colocalized with insulator proteinsNat Commun23403565ChIP was performed with ~4 × 107 cells. Cells were cross-linked with 1% formaldehyde for 10 min at room temperature. Nuclear lysates were sonicated to generate 200– 1000 bp DNA fragments. ChIP was then performed with 6 μL of Drosophila α-Myc antibodyFull Paper
2013Day, K;Waite, LL;Thalacker-Mercer, A;West, A;Bamman, MM;Brooks, JD;Myers, RM;Absher, D;Differential DNA methylation with age displays both common and dynamic features across human tissues that are influenced by CpG landscapeGenome Biol.24034465;... samples All tissue samples were collected according to protocols and guidelines at their respective institutions. Iron deficient anemia buffy coat samples were commercially available and collected by Conversant Bio (Huntsville, AL, USA). Blood DFull Paper
2013Kellner, WA;Van Bortle, K;Li, L;Ramos, E;Takenaka, N;Corces, VG;Distinct isoforms of the Drosophila Brd4 homologue are present at enhancers, promoters and insulator sitesNucleic Acids Res.23945939Illumina adaptors (Illumina Cat# PE-102–1001) were titrated according to prepared DNA ChIP sample concentration and ligated with T4 ligase (NEB Cat# M0202S). Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (NFull Paper
2013Xing, F;Kobayashi, A;Okuda, H;Watabe, M;Pai, SK;Pandey, PR;Hirota, S;Wilber, A;Mo, YY;Moore, BE;Liu, W;Fukuda, K;Iiizumi, M;Sharma, S;Liu, Y;Wu, K;Peralta, E;Watabe, K;Reactive astrocytes promote the metastatic growth of breast cancer stem-like cells by activating Notch signalling in brainEMBO Mol Med23495140... ocyte (UC1) was a kind gift from Dr Russell Piper (University of California-San Francisco). Primary human breast tumour cells which maintained in xenograft tumour of NOD/SCID mouse were obtained from Conversant Biologics, Inc. shRNA-expressing leFull Paper
2013Park, S;Balasubramaniam, V;Sastry, S;Estimating pressure induced changes in vegetable tissue using in situ electrical conductivity measurement and instrumental analysisJournal of Food EngineeringPittsfield, MA, USA). The ends of the copper wires were soldered to the high pressure electrical feed through and electrically insulated using polyolefin heat shrink tubing (Q2-Z-1/8, Qualtek Electronics, Mentor, OH, USA). A typeFull Paper
2013Okuda, H;Xing, F;Pandey, PR;Sharma, S;Watabe, M;Pai, SK;Mo, YY;Iiizumi-Gairani, M;Hirota, S;Liu, Y;Wu, K;Pochampally, R;Watabe, K;miR-7 suppresses brain metastasis of breast cancer stem-like cells by modulating KLF4Cancer Res.23384942mixture. Breast tumor cells that were directly transplanted to animal only one generation without in vitro culture were obtained from Conversant Biologics. Stem cell populations were isolated as described previously (14). Iso ;... odium pyruvateFull Paper
2013Sah, S;Chen, L;Houghton, J;Kemppainen, J;Marko, AC;Zeigler, R;Latham, GJ;Functional DNA quantification guides accurate next-generation sequencing mutation detection in formalin-fixed, paraffin-embedded tumor biopsiesGenome Med24001039Seventy-six FFPE blocks containing thyroid tumor biopsies were purchased from Asterand (Detroit, MI, USA). The remaining 39 FFPE tissue specimens were processed from colorectal cancer resections purchased from Folio Biosciences (Columbus, OH, USA) [1Full Paper
2013Soria, JC;Massard, C;Lazar, V;Ozoux, ML;Mery-Mignard, D;Deslandes, A;Tolcher, AW;A dose finding, safety and pharmacokinetic study of AVE1642, an anti-insulin-like growth factor-1 receptor (IGF-1R/CD221) monoclonal antibody, administered as a single agent and in combination with docetaxel in patients with advanced solid tumoursEur. J. Cancer23485230Tumour tissue: When possible, tumour tissue was collected before AVE1642 administration and at d21-d22 of cycle 1 to evaluate IGF-1R expression and signalling pathway markers, using validated immunohistochemistry assays, in Qualtek (USA)Full Paper
2013Rafaelsen, SH;Raeder, H;Fagerheim, AK;Knappskog, P;Carpenter, TO;Johansson, S;Bjerknes, R;Exome sequencing reveals FAM20c mutations associated with fibroblast growth factor 23-related hypophosphatemia, dental anomalies, and ectopic calcificationJ. Bone Miner. Res.23325605Genomic DNA was purified from blood using the QiaSymphony system (Qiagen, Hilden, Germany). Whole‐genome single‐nucleotide polymorphism (SNP) genotyping was performed with Genome‐Wide Human SNP 6.0 array (Affymetrix, Santa Clara, CA, USA). Whole‐exomFull Paper
2013Soler, VJ;Tran-Viet, KN;Galiacy, SD;Limviphuvadh, V;Klemm, TP;St Germain, E;Fournié, PR;Guillaud, C;Maurer-Stroh, S;Hawthorne, F;Suarez, C;Kantelip, B;Afshari, NA;Creveaux, I;Luo, X;Meng, W;Calvas, P;Cassagne, M;Arné, JL;Rozen, SG;Malecaze, F;Young, TL;Whole exome sequencing identifies a mutation for a novel form of corneal intraepithelial dyskeratosisJ. Med. Genet.23349227Seven micrograms of DNA were submitted to Hudson Alpha Laboratories (Huntsville, Alabama, USA) (http://www.hudsonalpha. org/) for deep sequencing processing to achieve an average coverage of 50× read depth. The libraries were prepared according to maFull Paper
2013Handorf, CR;Kulkarni, A;Grenert, JP;Weiss, LM;Rogers, WM;Kim, OS;Monzon, FA;Halks-Miller, M;Anderson, GG;Walker, MG;Pillai, R;Henner, WD;A multicenter study directly comparing the diagnostic accuracy of gene expression profiling and immunohistochemistry for primary site identification in metastatic tumorsAm. J. Surg. Pathol.23648464meeting the criteria were obtained as archived formalin-fixed, paraffin-embedded specimens from 4 institutions: the University of Tennessee (UT); Virginia Commonwealth University (VCU); Indiana University-Purdue University, Indianapolis (IUPUI); andFull Paper
2013Majumdar, S;Gong, EM;Di Vizio, D;Dreyfuss, J;Degraff, DJ;Hager, MH;Park, PJ;Bellmunt, J;Matusik, RJ;Rosenberg, JE;Adam, RM;Loss of Sh3gl2/endophilin A1 is a common event in urothelial carcinoma that promotes malignant behaviorNeoplasia23814487ILMN_1661491 [20]. Expression of Sh3gl2 protein was evaluated using commercially available bladder cancer tissue microarrays from either Folio Biosciences (Columbus, OH) or US Biomax, Inc (Rockville, MD). Specific immunohistochemicalFull Paper
2013Bowman, GL;Dodge, HH;Mattek, N;Barbey, AK;Silbert, LC;Shinto, L;Howieson, DB;Kaye, JA;Quinn, JF;Plasma omega-3 PUFA and white matter mediated executive decline in older adultsFront Aging Neurosci24379780Dr. Quinn has received honoraria for speaking from Pfizer, Novartis, and Forrest and for consulting from Phylogeny, Inc. Dr. Quinn is a co-inventor on a patent for the use of DHA for the treatment of Alzheimer's disease. Dr. Quinn receives compensatiFull Paper
2013Allaire, PD;Seyed Sadr, M;Chaineau, M;Seyed Sadr, E;Konefal, S;Fotouhi, M;Maret, D;Ritter, B;Del Maestro, RF;McPherson, PS;Interplay between Rab35 and Arf6 controls cargo recycling to coordinate cell adhesion and migrationJ. Cell. Sci.23264734Human brain tumor samples were obtained from the Montreal Neurological Institute Brain Tumor Tissue Bank. The experimental procedures were approved by the ethics committee of the Montreal Neurological Institute. The breast and squamous cell carcinomaFull Paper
2013Bohbot, JD;Durand, NF;Vinyard, BT;Dickens, JC;Functional Development of the Octenol Response in Aedes aegyptiFront Physiol23471139We wish to thank the following individuals: Dr. Shawn E. Levy and Nripesh Prasad at the Genomic Services Lab at HudsonAlpha Institute for Biotechnology for Illumina sequencing and data analyses; Dr. Gary Bauchan (The Electron and Confocal MicroscopeFull Paper
2013Premkumar, DR;Jane, EP;Agostino, NR;DiDomenico, JD;Pollack, IF;Bortezomib-induced sensitization of malignant human glioma cells to vorinostat-induced apoptosis depends on reactive oxygen species production, mitochondrial dysfunction, Noxa upregulation, Mcl-1 cleavage, and DNA damageMol. Carcinog.22086447time of tumor resection. We also obtained glioblastoma multiforme (GBM) primary cells from Conversant Biologics (Huntsville, AL), which were cultured in DMEM/F12 (Invitrogen, Carlsbad, CA). The established malignant gliomaFull Paper
2013Kinder, M;Greenplate, AR;Grugan, KD;Soring, KL;Heeringa, KA;McCarthy, SG;Bannish, G;Perpetua, M;Lynch, F;Jordan, RE;Strohl, WR;Brezski, RJ;Engineered protease-resistant antibodies with selectable cell-killing functionsJ. Biol. Chem.23986451Immunohistochemistry. All immunohistochemistry was performed by QualTek Molecular Laboratories (Newtown, PA). Four-micrometer sections were dewaxed through four changes of xylene (5 min each) followed by a graded alcohol series to distilled waterFull Paper
2013North, GB;Lynch, FH;Maharaj, FD;Phillips, CA;Woodside, WT;Leaf hydraulic conductance for a tank bromeliad: axial and radial pathways for moving and conserving waterFront Plant Sci23596446Full Paper
2013Park, S;Balasubramaniam, V;Sastry, S;Lee, J;Pressure–ohmic–thermal sterilization: A feasible approach for the inactivation of Bacillus amyloliquefaciens and Geobacillus stearothermophilus sporesInnovative Food Science & Emerging TechnologiesEach soldered part was electrically insulated using heat shrink tubing (Q2-F-1/16-01-QB6IN- 36, Qualtek, Mentor, OH, USA) and epoxy (2-t ® epoxy clear, Devcon, Danvers, MA, USA) to prevent short circuits and current loss to the pressure-transmittingFull Paper
2013Onsum, MD;Geretti, E;Paragas, V;Kudla, AJ;Moulis, SP;Luus, L;Wickham, TJ;McDonagh, CF;MacBeath, G;Hendriks, BS;Single-cell quantitative HER2 measurement identifies heterogeneity and distinct subgroups within traditionally defined HER2-positive patientsAm. J. Pathol.24035511Patient Samples. Sections (5 μm thick) of a breast disease spectrum TMA were obtained from Folio Biosciences (Powell, OH) (catalog number ARY-HH0137). Duplicate 1-mm tissue cores from 48 patients were represented on the TMAFull Paper
2013Liu, S;Wang, X;Sun, F;Zhang, J;Feng, J;Liu, H;Rajendran, KV;Sun, L;Zhang, Y;Jiang, Y;Peatman, E;Kaltenboeck, L;Kucuktas, H;Liu, Z;RNA-Seq reveals expression signatures of genes involved in oxygen transport, protein synthesis, folding, and degradation in response to heat stress in catfishPhysiol. Genomics23632418;Login to your account. Institutional Login. Sign In / Register. PHYSIOLOGY.ORG Skip main navigationClose Drawer MenuOpen Drawer Menu Home: Journals: AJP-Cell; AJP-Endo; AJP-GI; AJP-Heart; AJP-Lung; AJP-Regu; AJP-Renal; ; For each group, equalFull Paper
2013Kauffman, AL;Gyurdieva, AV;Mabus, JR;Ferguson, C;Yan, Z;Hornby, PJ;Alternative functional in vitro models of human intestinal epitheliaFront Pharmacol23847534n Majewski, Jason Ekert, and Kimberly Reese for help with iPSC maintenance culture. We also acknowledge the outstanding quality of immunohistochemistry performed by Steve Bernstein and Marko Sldanov, Qualtek Molecular Labs, Goleta, CA. References AlFull Paper
2013Lawley, B;Sims, IM;Tannock, GW;Whole-transcriptome shotgun sequencing (RNA-seq) screen reveals upregulation of cellobiose and motility operons of Lactobacillus ruminis L5 during growth on tetrasaccharides derived from barley β-glucanAppl. Environ. Microbiol.23851085Genomic comparisons were made in the expectation of understanding differential utilization of tetrasaccharides by L. ruminis strains. Paired-end reads were mapped to the publically available L. ruminis genomes: ATCC 25644 (did not utilize tetrasacchaFull Paper
2013Wang, R;Sun, L;Bao, L;Zhang, J;Jiang, Y;Yao, J;Song, L;Feng, J;Liu, S;Liu, Z;Bulk segregant RNA-seq reveals expression and positional candidate genes and allele-specific expression for disease resistance against enteric septicemia of catfishBMC Genomics24373586;... erformed using KAPA Library Quant Kits (Kapa Biosystems, USA) and dilution, the library were clustered 3 in one lane and sequenced on a Hiseq 2500 instrument with 100 bp paired end (PE) reads at the HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2013Xu, J;Li, Q;Xu, L;Wang, S;Jiang, Y;Zhao, Z;Zhang, Y;Li, J;Dong, C;Xu, P;Sun, X;Gene expression changes leading extreme alkaline tolerance in Amur ide (Leuciscus waleckii) inhabiting soda lakeBMC Genomics24094069;... ). Equal amounts of the high quality RNA samples from each tissue were then pooled for RNA-Seq. cDNA library construction and sequencing RNA-Seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2013Rokyta, DR;Wray, KP;Margres, MJ;The genesis of an exceptionally lethal venom in the timber rattlesnake (Crotalus horridus) revealed through comparative venom-gland transcriptomicsBMC Genomics23758969Sequencing and nonnormalized cDNA library preparation were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially as described by MortFull Paper
2013Sugihara, K;Shibata, TK;Takata, K;Kimura, T;Kanayama, N;Williams, R;Hatakeyama, S;Akama, TO;Kuo, CW;Khoo, KH;Fukuda, MN;Attenuation of fibroblast growth factor signaling by poly-N-acetyllactosamine type glycansFEBS Lett.23968720PolyLacs were collected and desalted using a Sephadex G-25 column and lyophilized. 2.7. Immunohistochemistry. Paraffin-embedded sections of human testis, epididymis, and ductus deferens tissues were obtained from Folio Biosciences (Columbus, OH)Full Paper
2013Gurudatta, BV;Yang, J;Van Bortle, K;Donlin-Asp, PG;Corces, VG;Dynamic changes in the genomic localization of DNA replication-related element binding factor during the cell cycleCell Cycle23624840The reaction was stopped by adding glycine to a final concentration of 0.125 mM. Chromatin was prepared by shearing the chromatin using sonication. Chromatin was immunoprecipitated using antibodies to DREF,35,40 and the DNA was used for library prepaFull Paper
2013Haugarvoll, K;Johansson, S;Tzoulis, C;Haukanes, BI;Bredrup, C;Neckelmann, G;Boman, H;Knappskog, PM;Bindoff, LA;MRI characterisation of adult onset alpha-methylacyl-coA racemase deficiency diagnosed by exome sequencingOrphanet J Rare Dis23286897Genealogical data from the patients, local parish registers and population censuses were used. Whole genome, single nucleotide polymorphism (SNP) genotyping was performed utilizing the Genome-Wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA) andFull Paper
2013O'Donnell, P;Ferguson, J;Shyu, J;Current, R;Rehage, T;Tsai, J;Christensen, M;Tran, HB;Chien, SS;Shieh, F;Wei, W;Lawrence, HJ;Wu, L;Schilling, R;Bloom, K;Maltzman, W;Anderson, S;Soviero, S;Analytic performance studies and clinical reproducibility of a real-time PCR assay for the detection of epidermal growth factor receptor gene mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancerBMC Cancer23621958(Wilmington, DE, USA), Asterand,Inc. (Detroit, MI, USA), BioServe (Beltsville, MD, USA), Conversant (Huntsville,AL, USA), Cureline Inc. (South San Francisco, CA, USA), Cytomyx (Lexington, MA,USA), Discovery Life Sciences, IncFull Paper
2013Peatman, E;Li, C;Peterson, BC;Straus, DL;Farmer, BD;Beck, BH;Basal polarization of the mucosal compartment in Flavobacterium columnare susceptible and resistant channel catfish (Ictalurus punctatus)Mol. Immunol.23895942; preserving the replicate pools for replicated QPCR validation. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25Full Paper
2013Sookruksawong, S;Sun, F;Liu, Z;Tassanakajon, A;RNA-Seq analysis reveals genes associated with resistance to Taura syndrome virus (TSV) in the Pacific white shrimp Litopenaeus vannameiDev. Comp. Immunol.23921257RNA-Seq library preparation and sequencing was outsourced and performed in the Genomic Services Lab of the HudsonAlpha Biotechnology Institute (Huntsville, AL, USA) as previously described (Li et al., 2012b; Sun et al., 2012). The cDNA libraries of TFull Paper
2013Macias, JD;Gerkin, RD;Locke, D;Macias, MP;Differential gene expression in cholesteatoma by DNA chip analysisLaryngoscope23670528From the Macias Otology (J.D.M.), Biomedical Research Program, the EAR Foundation of Arizona (J.D.M., M.P.M.), and Scientific Services, Banner Health Research Institute (R.D.G.), Phoenix, Arizona, USA; and QualTek Molecular Laboratories (D.L.), NewtoFull Paper
2013Infante, CR;Park, S;Mihala, AG;Kingsley, DM;Menke, DB;Pitx1 broadly associates with limb enhancers and is enriched on hindlimb cis-regulatory elementsDev. Biol.23201014Two independent biological replicates were used to generate two Illumina ChIP-Seq and two control libraries. All ChIP and input chromatin control libraries were produced using the Illumina ChIP-Seq DNA Sample Prep Kit (IP-102-1001) as directed by theFull Paper
2013Tzoulis, C;Johansson, S;Haukanes, BI;Boman, H;Knappskog, PM;Bindoff, LA;Novel SACS mutations identified by whole exome sequencing in a norwegian family with autosomal recessive spastic ataxia of Charlevoix-SaguenayPLoS ONE23785480Whole exome sequencing was performed at HudsonAlpha Institute for Biotechnology (Huntsville,AL) using Roche-NimbleGen Sequence Capture EZ Exome v2 kit and paired-end 100nt sequencing on the Illumina HiSeq [21].Full Paper
2013Xu, J;Ji, P;Wang, B;Zhao, L;Wang, J;Zhao, Z;Zhang, Y;Li, J;Xu, P;Sun, X;Transcriptome sequencing and analysis of wild Amur Ide (Leuciscus waleckii) inhabiting an extreme alkaline-saline lake reveals insights into stress adaptationPLoS ONE23573207;... lity RNA samples from each tissue were then pooled for cDNA synthesis and sequencing. cDNA Library Construction, Sequencing and Assembly RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2013Jiang, Y;Ninwichian, P;Liu, S;Zhang, J;Kucuktas, H;Sun, F;Kaltenboeck, L;Sun, L;Bao, L;Liu, Z;Generation of physical map contig-specific sequences useful for whole genome sequence scaffoldingPLoS ONE24205335One microliter ligation product was used as template for amplification with the following reaction cocktail: 1 µl 10X buffer, 1 U polymerase, 0.4 µl MgCl2 [50 mM], 0.8 µl dNTPs [2.5 mM], 2µl [5 µM] in-house-designed primers. Reactions were conductedFull Paper
2013Liu, L;Li, C;Su, B;Beck, BH;Peatman, E;Short-term feed deprivation alters immune status of surface mucosa in channel catfish (Ictalurus punctatus)PLoS ONE24023952;... ach condition, equal amounts of RNA from skin and gill were pooled to generate three pooled replicates for RNA-seq library construction. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2013Sun, F;Liu, S;Gao, X;Jiang, Y;Perera, D;Wang, X;Li, C;Sun, L;Zhang, J;Kaltenboeck, L;Dunham, R;Liu, Z;Male-biased genes in catfish as revealed by RNA-Seq analysis of the testis transcriptomePLoS ONE23874634;... Bioanalysis chip. Equal amount of total RNA from catfish testis of each age was pooled together into only one sample for use in RNA-Seq. RNA-Seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2013Garofalo, M;Jeon, YJ;Nuovo, GJ;Middleton, J;MiR-34a/c-dependent PDGFR-α/β downregulation inhibits tumorigenesis and enhances TRAIL-induced apoptosis in lung cancerPloS oneThe funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: GJN is an employee of Phylogeny, Inc. There are no patents, products in development or marketed productFull Paper
2013Lopez-Rios, F;Angulo, B;Gomez, B;Mair, D;Martinez, R;Conde, E;Shieh, F;Tsai, J;Vaks, J;Current, R;Lawrence, HJ;Gonzalez de Castro, D;Comparison of molecular testing methods for the detection of EGFR mutations in formalin-fixed paraffin-embedded tissue specimens of non-small cell lung cancerJ. Clin. Pathol.23386666Six FFPET specimens were purchased from INDIVUMED Inc. (Kensington, Maryland, USA), Cureline, Inc. (South San Francisco,California, USA), Bioserve (Beltsville, Maryland, USA), and ConversantBio (Huntsville, Alabama, USA). Study designFull Paper
2013Lamerz, J;Friedlein, A;Soder, N;Cutler, P;Döbeli, H;Determination of free desmosine in human plasma and its application in two experimental medicine studiesAnal. Biochem.23399390To validate the method, we used a plasma sample collection containing 30 patients with diagnosed COPD and 30 donors without a COPD diagnosis. This collection was obtained from Conversant Bio (Huntsville, AL, USA). ApartFull Paper
2013Pawar, H;Maharudraiah, J;Kashyap, MK;Sharma, J;Downregulation of cornulin in esophageal squamous cell carcinomaActa histochemicaThe ESCCs varied from tumor grade I to grade III from patients in the age group of 36-78 years. A second TMA set was obtained from FolioBio (Catalog no. ARY-HH0091, Powell, OH, USA) consisting of 40 ESCC cases with matched adjacent normal esophagealFull Paper
2013Adorno, M;Sikandar, S;Mitra, SS;Kuo, A;Nicolis Di Robilant, B;Haro-Acosta, V;Ouadah, Y;Quarta, M;Rodriguez, J;Qian, D;Reddy, VM;Cheshier, S;Garner, CC;Clarke, MF;Usp16 contributes to somatic stem-cell defects in Down's syndromeNature24025767Control bone marrow samples were obtained from AllCells, LLC. All samples were Control bone marrow samples were obtained from AllCells, LLC. Although we cannot unequivocally SaveFull Paper
2013Amir, el-AD;Davis, KL;Tadmor, MD;Simonds, EF;Levine, JH;Bendall, SC;Shenfeld, DK;Krishnaswamy, S;Nolan, GP;Pe'er, D;viSNE enables visualization of high dimensional single-cell data and reveals phenotypic heterogeneity of leukemiaNature biotechnology23685480Fresh, Ficoll-enriched human bone marrow was obtained from healthy donors from AllCells, Inc. (Emeryville, CA). Samples were obtained with informed consent in accordance with the SaveFull Paper
2013Meng, X;Su, RJ;Baylink, DJ;Neises, A;Kiroyan, JB;Lee, WY;Payne, KJ;Gridley, DS;Wang, J;Lau, KH;Li, G;Zhang, XB;Rapid and efficient reprogramming of human fetal and adult blood CD34+ cells into mesenchymal stem cells with a single factorCell research23478301University and written informed consent was obtained from all participants. CD34+ cells were purified with a CD34 Microbead Kit (Miltenyi Biotec, Auburn, CA, USA). Mobilized PB CD34+ cells were purchased from AllCells LLC (Emeryville, CA, USA)Full Paper
2013Yang, R;Liu, Y;Kelk, P;Qu, C;Akiyama, K;Chen, C;Atsuta, I;Chen, W;Zhou, Y;Shi, S;A subset of IL-17(+) mesenchymal stem cells possesses anti-Candida albicans effectCell research23266891Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells (Emeryville, CA, USA). Human bone marrow progenitor cells were enriched using fresh human bone marrow by RosetteSep® Human Bone Marrow ProgenitorFull Paper
2013Oshima, T;Sato, S;Kato, J;Ito, Y;Watanabe, T;Tsuji, I;Hori, A;Kurokawa, T;Kokubo, T;Nectin-2 is a potential target for antibody therapy of breast and ovarian cancersMolecular cancer23758976Human peripheral blood mononuclear cells (PBMC) from AllCells were cultured in RPMI1640 medium containing 10% FBS, 0.1 nM human IL-2 (DIACLONE Research), and 55 μM 2- SaveFull Paper
2013Takeishi, S;Matsumoto, A;Onoyama, I;Naka, K;Hirao, A;Nakayama, KI;Ablation of Fbxw7 eliminates leukemia-initiating cells by preventing quiescenceCancer cell23518349bone marrow mononuclear cells from treatment-naive patients in the chronic phase of CML and from healthy volunteers who had given informed consent were obtained from AllCells. SaveFull Paper
2013Deckert, J;Park, PU;Chicklas, S;Yi, Y;Li, M;Lai, KC;Mayo, MF;Carrigan, CN;Erickson, HK;Pinkas, J;Lutz, RJ;Chittenden, T;Lambert, JM;A novel anti-CD37 antibody-drug conjugate with multiple anti-tumor mechanisms for the treatment of B-cell malignanciesBlood24002446Frozen PBMCs from patients with CLL were obtained from AllCells (Emeryville, CA). For each sample, 5 × 10 5 cells were stained for indicated blood cell markers (Miltenyi) together with SaveFull Paper
2013Cutler, C;Multani, P;Robbins, D;Kim, HT;Le, T;Hoggatt, J;Pelus, LM;Desponts, C;Chen, YB;Rezner, B;Armand, P;Koreth, J;Glotzbecker, B;Ho, VT;Alyea, E;Isom, M;Kao, G;Armant, M;Silberstein, L;Hu, P;Soiffer, RJ;Scadden, DT;Ritz, J;Goessling, W;North, TE;Mendlein, J;Ballen, K;Zon, LI;Antin, JH;Shoemaker, DD;Prostaglandin-modulated umbilical cord blood hematopoietic stem cell transplantationBlood23996087Primary cells Cryopreserved human UCB CD34+ primary cells were purchased from AllCells or Stem Cell Technologies (Vancouver, BC, Canada)Full Paper
2013Malinge, S;Chlon, T;Doré, LC;Ketterling, RP;Tallman, MS;Paietta, E;Gamis, AS;Taub, JW;Chou, ST;Weiss, MJ;Crispino, JD;Figueroa, ME;Development of acute megakaryoblastic leukemia in Down syndrome is associated with sequential epigenetic changesBlood23980066Michigan, and the University of Michigan. Eight specimens of human CD34+ bone marrow cells were provided by the Stem Cell and Xenograft Core Facility of the University of Pennsylvania or purchased from AllCells (Emeryville, CA). These studies were peFull Paper
2013Kung Sutherland, MS;Walter, RB;Jeffrey, SC;Burke, PJ;Yu, C;Kostner, H;Stone, I;Ryan, MC;Sussman, D;Lyon, RP;Zeng, W;Harrington, KH;Klussman, K;Westendorf, L;Meyer, D;Bernstein, ID;Senter, PD;Benjamin, DR;Drachman, JG;McEarchern, JA;SGN-CD33A: a novel CD33-targeting antibody-drug conjugate using a pyrrolobenzodiazepine dimer is active in models of drug-resistant AMLBlood23770776For the primary AML xenograft model, NOD/SCID/IL-2Rgnull mice (NSG; The Jackson Laboratory, Bar Harbor, ME) were irradiated with 1 Gy 1 day before intravenous injection of 7 × 105 primary leukemia cells from a patient with relapsed AML (06227; AllCelFull Paper
2013Kojima, K;Kornblau, SM;Ruvolo, V;Dilip, A;Duvvuri, S;Davis, RE;Zhang, M;Wang, Z;Coombes, KR;Zhang, N;Qiu, YH;Burks, JK;Kantarjian, H;Shacham, S;Kauffman, M;Andreeff, M;Prognostic impact and targeting of CRM1 in acute myeloid leukemiaBlood23564911Normal marrow CD34 + cells from healthy donors aged 18 to 55 were obtained from AllCells (Emeryville, CA). The demographics and clinical characteristics of the patient population SaveFull Paper
2013Lee, J;Shieh, JH;Zhang, J;Liu, L;Zhang, Y;Eom, JY;Morrone, G;Moore, MA;Zhou, P;Improved ex vivo expansion of adult hematopoietic stem cells by overcoming CUL4-mediated degradation of HOXB4Blood23520338Each irradiated mouse (5 mice/group) was transplanted via tail vein injection with 3 × 105 CD34+ G-CSF-mobilized adult HSCs (AllCells, LLC, Emeryville, CA) treated with 2 rounds of purified recombinant wild-type or degradation-resistant HOXB4 proteinFull Paper
2013Bhagat, TD;Zhou, L;Sokol, L;Kessel, R;Caceres, G;Gundabolu, K;Tamari, R;Gordon, S;Mantzaris, I;Jodlowski, T;Yu, Y;Jing, X;Polineni, R;Bhatia, K;Pellagatti, A;Boultwood, J;Kambhampati, S;Steidl, U;Stein, C;Ju, W;Liu, G;Kenny, P;List, A;Bitzer, M;Verma, A;miR-21 mediates hematopoietic suppression in MDS by activating TGF-β signalingBlood23390194Bone marrow CD34+ cells from various normal donors were also obtained from AllCells (Emeryville, CA). HS-5 cell line (CRL-11882) was purchased from ATCC (Manassas, VA). TGF-β1 was bought from R&D Systems (Minneapolis, MN). The phos-SMAD2 (S465/467) aFull Paper
2013Kumkhaek, C;Aerbajinai, W;Liu, W;Zhu, J;Uchida, N;Kurlander, R;Hsieh, MM;Tisdale, JF;Rodgers, GP;MASL1 induces erythroid differentiation in human erythropoietin-dependent CD34+ cells through the Raf/MEK/ERK pathwayBlood23327923CD34+ cells were obtained from commercial sources (Lonza, Walkerville, MD, or AllCells, Emeryville, CA). Cells were thawed and washed into StemSpan serum-free expansion medium (SFEM) (StemCell Technologies, Vancouver, BC, Canada) and then seeded in SFull Paper
2013Wong, KY;Yim, RL;Kwong, YL;Leung, CY;Hui, PK;Cheung, F;Liang, R;Jin, DY;Chim, CS;Epigenetic inactivation of the MIR129-2 in hematological malignanciesJournal of hematology & oncology23406679MIR124-1 and MIR203 were previously described [14,16]. Mononuclear cell DNA from 15 healthy donors [8 bone marrow, 3 peripheral blood, 3 CD19-sorted peripheral blood, and 1 CD138-sorted bone marrow (AllCells, CA, USA)] were used as negative control,Full Paper
2013Richardson, CL;Delehanty, LL;Bullock, GC;Rival, CM;Tung, KS;Kimpel, DL;Gardenghi, S;Rivella, S;Goldfarb, AN;Isocitrate ameliorates anemia by suppressing the erythroid iron restriction responseThe Journal of clinical investigation23863711The unique sensitivity of early red cell progenitors to iron deprivation, known as the erythroid iron restriction response, serves as a basis for human anemias globally. This response SaveFull Paper
2013McBrian, MA;Behbahan, IS;Ferrari, R;Su, T;Huang, TW;Li, K;Hong, CS;Christofk, HR;Vogelauer, M;Seligson, DB;Kurdistani, SK;Histone acetylation regulates intracellular pHMolecular cell23201122H1 cells were maintained in mTeSR1 medium (STEMCELL Technologies #05850) on Matrigel. T cells were purchased from AllCells (PB009-1F) and were maintained in RPMI-1640 (ATCC #30-2001) with 10% FBS (Hyclone #SV3001403) that was heat inactivated by treaFull Paper
2013Kuroda, Y;Wakao, S;Kitada, M;Murakami, T;Nojima, M;Dezawa, M;Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cellsNature protocols23787896For example, when we purchased bone marrow from ALLCELLS, which is imported from the United States to Japan and typically takes 5-7 d to arrive, the number of mononuclear cells SaveFull Paper
2013Cheng, DT;Ma, C;Niewoehner, J;Dahl, M;Tsai, A;Zhang, J;Gonsiorek, W;Apparsundaram, S;Pashine, A;Ravindran, P;Jung, J;Hang, J;Allard, J;Bitter, H;Tribouley, C;Narula, S;Wilson, S;Fuentes, ME;Thymic stromal lymphopoietin receptor blockade reduces allergic inflammation in a cynomolgus monkey model of asthmaThe Journal of allergy and clinical immunology23810153Single donor human cord blood-derived CD34 + cells were purchased from AllCells (Emeryville, Calif). Cells were cultured for 8 weeks in serum-free complete medium (StemPro-34 SaveFull Paper
2013Leone, E;Morelli, E;Di Martino, MT;Amodio, N;Foresta, U;Gullà, A;Rossi, M;Neri, A;Giordano, A;Munshi, NC;Anderson, KC;Tagliaferri, P;Tassone, P;Targeting miR-21 inhibits in vitro and in vivo multiple myeloma cell growthClinical cancer research : an official journal of the American Association for Cancer Research23446999Imgenex) by standard procedures (37, 38). hBMSCs were obtained by long-term culture of bone marrow mononuclear cells (39). For coculture, 1 × 105 ppMM cells were seeded on 2 × 104 hBMSCs for 24 to 48 hours in 96-well plates. RNA samples of normal heaFull Paper
2013Kuhne, MR;Mulvey, T;Belanger, B;Chen, S;Pan, C;Chong, C;Cao, F;Niekro, W;Kempe, T;Henning, KA;Cohen, LJ;Korman, AJ;Cardarelli, PM;BMS-936564/MDX-1338: a fully human anti-CXCR4 antibody induces apoptosis in vitro and shows antitumor activity in vivo in hematologic malignanciesClinical cancer research : an official journal of the American Association for Cancer Research23213054Binding Buffer 10× concentrate, 7-aminoactinomycin D (7-AAD), and Annexin V-APC from BD Biosciences; phycoerythrin (PE)-conjugated goat anti-human antibody from Jackson ImmunoResearch (Cat. 109-116-098). AML peripheral blood mononuclear cells (PBMC)Full Paper
2013Corbin, AS;O'Hare, T;Gu, Z;Kraft, IL;Eiring, AM;Khorashad, JS;Pomicter, AD;Zhang, TY;Eide, CA;Manley, PW;Cortes, JE;Druker, BJ;Deininger, MW;KIT signaling governs differential sensitivity of mature and primitive CML progenitors to tyrosine kinase inhibitorsCancer research23887971Normal bone marrow mononuclear cells (MNC) were from AllCells. Cell selection was as described previously (details in Supplementary Methods; ref. 17). SaveFull Paper
2013Ara, T;Nakata, R;Sheard, MA;Shimada, H;Buettner, R;Groshen, SG;Ji, L;Yu, H;Jove, R;Seeger, RC;DeClerck, YA;Critical role of STAT3 in IL-6-mediated drug resistance in human neuroblastomaCancer research23633489AmpFISTR Identifier PCR kit and GeneMapper ID v. 3.2 (Applied Biosystems). Human BMMSC were purchased from AllCells LLC. Monocytes of normal healthy donors were obtained from peripheral blood and separated by Ficoll density gradient centrifugation usFull Paper
2013Wei, Y;Chen, R;Dimicoli, S;Bueso-Ramos, C;Neuberg, D;Pierce, S;Wang, H;Yang, H;Jia, Y;Zheng, H;Fang, Z;Nguyen, M;Ganan-Gomez, I;Ebert, B;Levine, R;Kantarjian, H;Garcia-Manero, G;Global H3K4me3 genome mapping reveals alterations of innate immunity signaling and overexpression of JMJD3 in human myelodysplastic syndrome CD34+ cellsLeukemia23538751BMs from healthy individuals were obtained from AllCells (Emeryville, CA, USA). Isolation of CD34+ cells was performed using MicroBead Kit (Miltenyi, Bergisch Gladbach, Germany) SaveFull Paper
2013Rio-Machin, A;Ferreira, BI;Henry, T;Gómez-López, G;Agirre, X;Alvarez, S;Rodriguez-Perales, S;Prosper, F;Calasanz, MJ;Martínez, J;Fonseca, R;Cigudosa, JC;Downregulation of specific miRNAs in hyperdiploid multiple myeloma mimics the oncogenic effect of IgH translocations occurring in the non-hyperdiploid subtypeLeukemia23174883CD138-positive snap-frozen plasma cell pellets (ALLCELLS, Emeryville, CA, USA) were DNA from healthy CD138+ snap-frozen plasma cell pellets (ALLCELLS, Emeryville, CA, USA) SaveFull Paper
2013Díaz-Beyá, M;Navarro, A;Ferrer, G;Díaz, T;Gel, B;Camós, M;Pratcorona, M;Torrebadell, M;Rozman, M;Colomer, D;Monzo, M;Esteve, J;Acute myeloid leukemia with translocation (8;16)(p11;p13) and MYST3-CREBBP rearrangement harbors a distinctive microRNA signature targeting RET proto-oncogeneLeukemia23022987In addition, CD34+ cells from the bone marrow of three healthy donor subjects were purchased from AllCells (Emeryville, CA, USA). All patients provided their written informed consent SaveFull Paper
2013Hayashi, Y;Hirai, H;Kamio, N;Yao, H;Yoshioka, S;Miura, Y;Ashihara, E;Fujiyama, Y;Tenen, DG;Maekawa, T;C/EBPβ promotes BCR-ABL-mediated myeloid expansion and leukemic stem cell exhaustionLeukemia22948537Frozen samples of human bone marrow cells from healthy donors or untreated CP-CML patients were purchased from AllCells LLC (Emeryville, CA, USA). In all cases, written informed Save;[方紘] 慢性期 CML 患者および健常人ドナーの骨髄細胞検体は AllCells 社より購入した. マウス 血幹細胞の細胞 でFull Paper
2013Lin, X;Rice, KL;Buzzai, M;Hexner, E;Costa, FF;Kilpivaara, O;Mullally, A;Soares, MB;Ebert, BL;Levine, R;Licht, JD;miR-433 is aberrantly expressed in myeloproliferative neoplasms and suppresses hematopoietic cell growth and differentiationLeukemia22864358Human CD34+ cells were obtained from adult bone marrow (AllCells, Emeryville, CA, USA) and cultured in StemSpan serum-free expansion media (SFEM; Stem Cell Technologies, SaveFull Paper
2013Shin, JW;Spinler, KR;Swift, J;Chasis, JA;Mohandas, N;Discher, DE;Lamins regulate cell trafficking and lineage maturation of adult human hematopoietic cellsProceedings of the National Academy of Sciences of the United States of America24191023Commercially available primary human cells from marrow were obtained from either AllCells, Inc. (Emeryville, CA) or anonymous donors to the University of Pennsylvania Stem Cell SaveFull Paper
2013Xie, J;Zhang, H;Yea, K;Lerner, RA;Autocrine signaling based selection of combinatorial antibodies that transdifferentiate human stem cellsProceedings of the National Academy of Sciences of the United States of America23613575Differentiation of CD34+ Cells in Culture. Fresh bone marrow CD34+ cells were purchased from Allcells (catalog no. ABM010). The purity is greater than 96% as determined by FACS. The cells were washed with SFEM (Stem Cell 09600) and kept in SFEM withFull Paper
2013Hong, KU;Li, QH;Guo, Y;Patton, NS;Moktar, A;Bhatnagar, A;Bolli, R;A highly sensitive and accurate method to quantify absolute numbers of c-kit+ cardiac stem cells following transplantation in miceBasic research in cardiology23549981Female human peripheral mononuclear cells (hPBMCs) were purchased from AllCells, LLC (Emeryville, CA). Aliquots of 105 hPBMCs (in 50 μl PBS) were stored at −20 °C until use. For isolation of genomic DNA, QIAamp DNA Mini Kit (Qiagen) was used accordinFull Paper
2013Ramakrishnan, P;Clark, PM;Mason, DE;Peters, EC;Hsieh-Wilson, LC;Baltimore, D;Activation of the transcriptional function of the NF-κB protein c-Rel by O-GlcNAc glycosylationScience signaling23982206Biotechnology. The anti-FLAG antibody was from Sigma. The anti-O-GlcNAc antibodies RL2 and CTD 110.6 were from Abcam and Covance, respectively. Cells Jurkat, Jurkat T-REx, Ramos, EL4, MEFs, and HEK 293T cells were grown as previously described (23).Full Paper
2013Isgro, J;Gupta, S;Jacek, E;Pavri, T;Duculan, R;Kim, M;Kirou, KA;Salmon, JE;Pernis, AB;Enhanced rho-associated protein kinase activation in patients with systemic lupus erythematosusArthritis and rheumatism23508371Cryopreserved vials of human cord blood CD4+ T lymphocytes were obtained from Lonza Poietics, Immune Cell Systems, and AllCells, LLC. CD4+ T cell purity was 95%. Cells were thawed according to manufacturer’s instructions and cultured in LGM-3 (Lonza)Full Paper
2013Allakhverdi, Z;Comeau, MR;Armant, M;Agrawal, R;Woodfolk, JA;Sehmi, R;Howie, KJ;Gauvreau, GM;Delespesse, G;Mast Cell-Activated Bone Marrow Mesenchymal Stromal Cells Regulate Proliferation and Lineage Commitment of CD34(+) Progenitor CellsFrontiers in immunology24381572ibody were used at 10 μg/ml, as in our previous study (4). Primary human bone marrow-derived mesenchymal stromal cells Human BM-derived mesenchymal stromal cells were established from BM samples (AllCells, Emeryville, CA, USA) by culture in minimumFull Paper
2013Wu, Q;Wang, H;Zhao, X;Shi, Y;Jin, M;Wan, B;Xu, H;Cheng, Y;Ge, H;Zhang, Y;Identification of G-protein-coupled receptor 120 as a tumor-promoting receptor that induces angiogenesis and migration in human colorectal carcinomaOncogene23851494ECMatrix (50 μl, Merck KGaA ) was distributed per well in a 96-well plate. HUVECs (ALLCELLS, Shanghai, China, 2 × 104 cells/well) suspended in 100 μl of medium supplemented with 0.5% FBS were added to each well and cultured in triplicate with 150 μlFull Paper
2013Li, C;Takahashi, C;Zhang, L;Huseni, M;Stankovich, B;Mashhedi, H;Lee, J;French, D;Anderson, JE;Kim, D;Howell, K;Brauer, MJ;Kowanetz, M;Yan, Y;Humke, E;Ebens, A;Hampton, G;Lackner, MR;Hegde, P;Jia, S;Development of a robust flow cytometry-based pharmacodynamic assay to detect phospho-protein signals for phosphatidylinositol 3-kinase inhibitors in multiple myelomaJournal of translational medicine23522020le myeloma Li Congfen 1 li.congfen@gene.com Takahashi Chikara 1 takahashi.chikara@gene.com Zhang Liangxuan 1 zhang.liangxuan@gene.com Huseni Mahrukh 1 mahrukhh@gene.com Stankovich Basha 2 bstankovich@allcells.com Mashhedi Haider 1 mashhedh@gene.com LFull Paper
2013Nazarov, C;Lo Surdo, J;Bauer, SR;Wei, CH;Assessment of immunosuppressive activity of human mesenchymal stem cells using murine antigen specific CD4 and CD8 T cells in vitroStem cell research & therapy24406271by the Association for Assessment and Accreditation of Laboratory Animal Care International. All experiments were performed according to institutional guidelines. Cell culture hMSCs were purchased (AllCells, Emeryville, CA, USA) at passage one. AccoFull Paper
2013Ledford, KJ;Zeigler, F;Bartel, RL;Ixmyelocel-T, an expanded multicellular therapy, contains a unique population of M2-like macrophagesStem cell research & therapy24405629For the generation of M1 and M2 macrophages, human peripheral blood monocytes from healthy donors were purchased (AllCells LLC., Alameda, USA). The cells were polarized into SaveFull Paper
2013Vanella, L;Sodhi, K;Kim, DH;Puri, N;Maheshwari, M;Hinds, TD;Bellner, L;Goldstein, D;Peterson, SJ;Shapiro, JI;Abraham, NG;Increased heme-oxygenase 1 expression in mesenchymal stem cell-derived adipocytes decreases differentiation and lipid accumulation via upregulation of the canonical Wnt signaling cascadeStem cell research & therapy23497794Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA, USA). After thawing, mononuclear cells were resuspended in an α-minimal essential SaveFull Paper
2013McNamara, LA;Onafuwa-Nuga, A;Sebastian, NT;Riddell, J;Bixby, D;Collins, KL;CD133+ hematopoietic progenitor cells harbor HIV genomes in a subset of optimally treated people with long-term viral suppressionThe Journal of infectious diseases23554378and 7-aminoactinomycin D (7-AAD). Healthy donor BMMCs (AllCells) were stained with R-phycoerythrin-conjugated anti-CD133, R-phycoerythrin-Cy7-conjugated anti-CD34 (BD), and 7-AAD. Samples were analyzed on a BD FACSCantoFull Paper
2013Moy, LY;Jia, Y;Caniga, M;Lieber, G;Gil, M;Fernandez, X;Sirkowski, E;Miller, R;Alexander, JP;Lee, HH;Shin, JD;Ellis, JM;Chen, H;Wilhelm, A;Yu, H;Vincent, S;Chapman, RW;Kelly, N;Hickey, E;Abraham, WM;Northrup, A;Miller, T;Houshyar, H;Crackower, MA;Inhibition of spleen tyrosine kinase attenuates allergen-mediated airway constrictionAmerican journal of respiratory cell and molecular biology23889698+ cells (5 x 10 6 /vial, AllCells) were thawed and added into 50 ml polypropylene conical tube with 100 µL of 1mg/ml DNase I (StemCell). 15.5 ml of pre-warmed Dulbecco's modified SaveFull Paper
2013Ye, L;Muench, MO;Fusaki, N;Beyer, AI;Wang, J;Qi, Z;Yu, J;Kan, YW;Blood cell-derived induced pluripotent stem cells free of reprogramming factors generated by Sendai viral vectorsStem cells translational medicine23847002Frozen human peripheral blood mobilized CD34+ cells were purchased from AllCells (Emeryville, CA, http://www.allcells.com). After thawing, CD34+ cells were cultured with serum-free medium (SFM) as described by Ye and coworkers [24, 29], containing 50Full Paper
2013Hall, SR;Jiang, Y;Leary, E;Yavanian, G;Eminli, S;O'Neill, DW;Marasco, WA;Identification and isolation of small CD44-negative mesenchymal stem/progenitor cells from human bone marrow using elutriation and polychromatic flow cytometryStem cells translational medicine23847000Fresh, mobilized leukapheresis products were purchased from AllCells (Emeryville, CA, http://www.allcells.com) or collected from healthy volunteers at NeoStem Laboratory (Cambridge, MA, http://www.neostem.com) under an institutional review board-apprFull Paper
2013Mendelson, A;Cheung, Yk;Paluch, K;Chen, M;Kong, K;Tan, J;Dong, Z;Sia, SK;Mao, JJ;Competitive stem cell recruitment by multiple cytotactic cuesLab on a chip23364311Human bone marrow MSCs were isolated from fresh adult donor samples (AllCells, Emeryville, CA) using our prior methods. Briefly, the bone marrow sample was incubated with the SaveFull Paper
2013Cairo, C;Sagnia, B;Cappelli, G;Colizzi, V;Leke, RG;Leke, RJ;Pauza, CD;Human cord blood γδ T cells expressing public Vγ2 chains dominate the response to bisphosphonate plus interleukin-15Immunology23181340fraction of CBMC was reserved for cell culture and 0·5 × 107 to 1 × 107 were lysed for RNA extraction; remaining cells were frozen at 1 × 107 CBMC/ml, in 90% fetal bovine serum (FBS), 10% DMSO freezing medium. Some CBMC specimens were purchased fromFull Paper
2013Kim, DH;Puri, N;Sodhi, K;Falck, JR;Abraham, NG;Shapiro, J;Schwartzman, ML;Cyclooxygenase-2 dependent metabolism of 20-HETE increases adiposity and adipocyte enlargement in mesenchymal stem cell-derived adipocytesJournal of lipid research23293373Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α-MEM, Save;ates: hypertension, the metabolic syndrome, and diabetes. MATERIALSFull Paper
2013Luo, Y;Kleiboeker, S;Deng, X;Qiu, J;Human parvovirus B19 infection causes cell cycle arrest of human erythroid progenitors at late S phase that favors viral DNA replicationJournal of virology24049177Cells and virus. (i) CD36+ EPCs. Human bone marrow CD34+ hematopoietic stem/progenitor cells (HSCs) were positively isolated using a direct immunomagnetic CD34+ MicroBead labeling system and were purchased from AllCells, LLC (Alameda, CA; catalog noFull Paper
2013Shen, Y;Zeng, L;Zhu, A;Blanc, T;Patel, D;Pennello, A;Bari, A;Ng, S;Persaud, K;Kang, YK;Balderes, P;Surguladze, D;Hindi, S;Zhou, Q;Ludwig, DL;Snavely, M;Removal of a C-terminal serine residue proximal to the inter-chain disulfide bond of a human IgG1 lambda light chain mediates enhanced antibody stability and antibody dependent cell-mediated cytotoxicitymAbs23567210drogenase (LDH) activity from the lysed targeted cells (A431 transfected with receptor target X, A431x) was monitored in the presence freshly isolated normal peripheral blood mononuclear cells (PBMC, AllCells, PB002) upon the treatment with λ or λdSFull Paper
2013Zhao, Y;Wang, J;Wang, M;Sun, P;Chen, J;Jin, X;Zhang, H;Activation of bone marrow-derived mesenchymal stromal cells-a new mechanism of defocused low-energy shock wave in regenerative medicineCytotherapy24199590Human umbilical vein endothelial cells (HUVEC) were purchased from Allcells (Shanghai, China) and maintained in EGM2 medium (CC-3162, Lonza, Basel, Switzerland). HUVEC SaveFull Paper
2013Jones, M;Varella-Garcia, M;Skokan, M;Bryce, S;Schowinsky, J;Peters, R;Vang, B;Brecheisen, M;Startz, T;Frank, N;Nankervis, B;Genetic stability of bone marrow-derived human mesenchymal stromal cells in the Quantum SystemCytotherapy23992670Initially, six human bone marrow aspirates supplied by normal adult donors, who gave informed consent through a certified clinical collection facility (AllCells, Alameda, CA, USA), were SaveFull Paper
2013Glen, KE;Workman, VL;Ahmed, F;Ratcliffe, E;Stacey, AJ;Thomas, RJ;Production of erythrocytes from directly isolated or Delta1 Notch ligand expanded CD34+ hematopoietic progenitor cells: process characterization, monitoring and implications for manufactureCytotherapy23831361Background aims Economic ex vivo manufacture of erythrocytes at 10 12 cell doses requires an efficiently controlled bio-process capable of extensive proliferation and high terminal SaveFull Paper
2013Song, L;Kauss, MA;Kopin, E;Chandra, M;Ul-Hasan, T;Miller, E;Jayandharan, GR;Rivers, AE;Aslanidi, GV;Ling, C;Li, B;Ma, W;Li, X;Andino, LM;Zhong, L;Tarantal, AF;Yoder, MC;Wong, KK;Tan, M;Chatterjee, S;Srivastava, A;Optimizing the transduction efficiency of capsid-modified AAV6 serotype vectors in primary human hematopoietic stem cells in vitro and in a xenograft mouse model in vivoCytotherapy23830234CD34+ and umbilical cord blood CD34+ cells were either purchased from AllCells or obtained under a City of Hope Institutional Review Board approved protocol isolated as described by Chatterjee et al. [7], and maintained in either StemSpan SFEM mediumFull Paper
2013Blackwood, E;Epler, J;Yen, I;Flagella, M;O'Brien, T;Evangelista, M;Schmidt, S;Xiao, Y;Choi, J;Kowanetz, K;Ramiscal, J;Wong, K;Jakubiak, D;Yee, S;Cain, G;Gazzard, L;Williams, K;Halladay, J;Jackson, PK;Malek, S;Combination drug scheduling defines a "window of opportunity" for chemopotentiation of gemcitabine by an orally bioavailable, selective ChK1 inhibitor, GNE-900Molecular cancer therapeutics23873850For the bone marrow assay, fresh human bone marrow-derived cells were obtained from AllCells and plated at a density of 1 million cells/mL in Human Methylcellulose Complete SaveFull Paper
2013Jekle, A;Yoon, J;Zuck, M;Najafi, R;Wang, L;Shiau, T;Francavilla, C;Rani, SA;Eitzinger, C;Nagl, M;Anderson, M;Debabov, D;NVC-422 inactivates Staphylococcus aureus toxinsAntimicrobial agents and chemotherapy23208720Normal human peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA) and maintained in RPMI 1640 medium supplemented with 10% fetal bovine SaveFull Paper
2013Winkler, DG;Faia, KL;DiNitto, JP;Ali, JA;White, KF;Brophy, EE;Pink, MM;Proctor, JL;Lussier, J;Martin, CM;Hoyt, JG;Tillotson, B;Murphy, EL;Lim, AR;Thomas, BD;Macdougall, JR;Ren, P;Liu, Y;Li, LS;Jessen, KA;Fritz, CC;Dunbar, JL;Porter, JR;Rommel, C;Palombella, VJ;Changelian, PS;Kutok, JL;PI3K-δ and PI3K-γ inhibition by IPI-145 abrogates immune responses and suppresses activity in autoimmune and inflammatory disease modelsChemistry & biology24211136Primary Human B and T Cell Proliferation Assays Human peripheral blood CD19+ B cells and CD3+ T cells were purchased from Allcells and proliferation assays were conducted as described in Supplemental Experimental ProceduresFull Paper
2013Liao, C;Hsu, J;Kim, Y;Hu, DQ;Xu, D;Zhang, J;Pashine, A;Menke, J;Whittard, T;Romero, N;Truitt, T;Slade, M;Lukacs, C;Hermann, J;Zhou, M;Lucas, M;Narula, S;DeMartino, J;Tan, SL;Selective inhibition of spleen tyrosine kinase (SYK) with a novel orally bioavailable small molecule inhibitor, RO9021, impinges on various innate and adaptive immune responses: implications for SYK inhibitors in autoimmune disease therapyArthritis research & therapy24286216y kits (BD Biosciences). IgE-induced histamine release in human mast cells The method has been reported previously by our group [9]. Briefly, human cord blood-derived CD34+ hematopoietic stem cells (AllCells, Emeryville, CA, USA) were cultured in aFull Paper
2013Tsai, HC;Wu, R;Cholera toxin directly enhances IL-17A production from human CD4+ T cellsJournal of immunology (Baltimore, Md. : 1950)24043897PBMCs were purchased from AllCells. According to the manufacturers' protocols, total CD4 + T cells were positively selected from PBMCs using magnetic CD4 MicroBeads (Miltenyi SaveFull Paper
2013Sohn, SJ;Barrett, K;Van Abbema, A;Chang, C;Kohli, PB;Kanda, H;Smith, J;Lai, Y;Zhou, A;Zhang, B;Yang, W;Williams, K;Macleod, C;Hurley, CA;Kulagowski, JJ;Lewin-Koh, N;Dengler, HS;Johnson, AR;Ghilardi, N;Zak, M;Liang, J;Blair, WS;Magnuson, S;Wu, LC;A restricted role for TYK2 catalytic activity in human cytokine responses revealed by novel TYK2-selective inhibitorsJournal of immunology (Baltimore, Md. : 1950)23894201IL-23 assay. IL-23 was obtained from R&D Systems and was used at a concentration that generated ∼50-90% of the maximal IL-23-induced signal in the assay (i.e., EC50-90 of IL-23). CD4 CD45RO+ T cells obtained from AllCells (Emeryville, CA) were culturFull Paper
2013Yokley, BH;Selby, ST;Posch, PE;A stimulation-dependent alternate core promoter links lymphotoxin α expression with TGF-β1 and fibroblast growth factor-7 signaling in primary human T cellsJournal of immunology (Baltimore, Md. : 1950)23547113Human T cells were purchased (Allcells, Emeryville, CA, USA) or were purified from PBMCs isolated from fresh whole blood with lymphocyte separation medium (Ficoll® and hypaque; Mediatech Inc, Manassas, VA, USA). Briefly, whole blood was brought to roFull Paper
2013Fitzgerald, DC;Fonseca-Kelly, Z;Cullimore, ML;Safabakhsh, P;Saris, CJ;Zhang, GX;Rostami, A;Independent and interdependent immunoregulatory effects of IL-27, IFN-β, and IL-10 in the suppression of human Th17 cells and murine experimental autoimmune encephalomyelitisJournal of immunology (Baltimore, Md. : 1950)23455508PBMCs were isolated using Ficoll-Paque Plus density centrifugation; cord blood cells were obtained from AllCells. CD4 + and CD14 + cells were purified by immunomagnetic SaveFull Paper
2013Heuck, CJ;Mehta, J;Bhagat, T;Gundabolu, K;Yu, Y;Khan, S;Chrysofakis, G;Schinke, C;Tariman, J;Vickrey, E;Pulliam, N;Nischal, S;Zhou, L;Bhattacharyya, S;Meagher, R;Hu, C;Maqbool, S;Suzuki, M;Parekh, S;Reu, F;Steidl, U;Greally, J;Verma, A;Singhal, SB;Myeloma is characterized by stage-specific alterations in DNA methylation that occur early during myelomagenesisJournal of immunology (Baltimore, Md. : 1950)23408834Eight samples from normal donors without known malignancies were obtained commercially from AllCells (Emeryville, CA). All normal donor samples had a purity of ≥85%. SaveFull Paper
2013Jiang, Z;Hu, X;Yu, H;Xu, Y;Wang, L;Chen, H;Chen, H;Wu, R;Zhang, Z;Xiang, C;Webster, KA;Wang, JA;Human endometrial stem cells confer enhanced myocardial salvage and regeneration by paracrine mechanismsJournal of cellular and molecular medicine23837896the supplemental information. Cell sources Human BMMSCs from three donors were purchased from Cyagen Bioscience (Shanghai, China). Human umbilical vein endothelial cells (HUVECs) were purchased from AllCells (Shanghai, China). Human EnSCs from threeFull Paper
2013He, X;Xu, Z;Wang, B;Zheng, Y;Gong, W;Huang, G;Zhang, L;Li, Y;He, F;Upregulation of thrombomodulin expression by activation of farnesoid X receptor in vascular endothelial cellsEuropean journal of pharmacology24041925The primary human umbilical vein endothelial cells (HUVECs) were purchased from AllCells, LLC (Shanghai, China) and were cultured in the medium supplied by AllCells with 10% SaveFull Paper
2013Larusch, GA;Merkulova, A;Mahdi, F;Shariat-Madar, Z;Sitrin, RG;Cines, DB;Schmaier, AH;Domain 2 of uPAR regulates single-chain urokinase-mediated angiogenesis through β1-integrin and VEGFR2American journal of physiology. Heart and circulatory physiology23709605HUVECs were cultured in 96-well microtiter plates on gelatin at 1 × 10 4 cells/well for 24 h in EGM from AllCells. Cells were then washed with serum-free medium without growth factor SaveFull Paper
2013Chen, Y;Chitapanarux, T;Wu, J;Soon, RK;Melton, AC;Yee, HF;Inducible NOS mediates CNP-induced relaxation of intestinal myofibroblastsAmerican journal of physiology. Gastrointestinal and liver physiology23348803NPR Western blots were performed by AllCells (Emeryville, CA) with the following antibodies: anti-mouse NPR-A (clone ab116680; Abcam, Cambridge, MA), anti-mouse NPR-B (clone SaveFull Paper
2013Koestler, DC;Christensen, B;Karagas, MR;Marsit, CJ;Langevin, SM;Kelsey, KT;Wiencke, JK;Houseman, EA;Blood-based profiles of DNA methylation predict the underlying distribution of cell types: a validation analysisEpigenetics23903776As previously described,10,18 our reference set (S0) consisted of sorted, normal, human, peripheral blood leukocyte subtypes purchased from AllCells. Leukocytes were isolated from different, anonymous, non-diseased individuals’ whole-blood by magnetiFull Paper
2013Harb, W;Fan, A;Tran, T;Danila, DC;Keys, D;Schwartz, M;Ionescu-Zanetti, C;Mutational Analysis of Circulating Tumor Cells Using a Novel Microfluidic Collection Device and qPCR AssayTranslational oncology24151533Healthy donor peripheral blood was obtained from a commercial supplier (AllCells, Emeryville, CA) under Institutional Review Board (IRB) consent. Blood was drawn into EDTA tubes ( SaveFull Paper
2013Shahaduzzaman, M;Golden, JE;Green, S;Gronda, AE;Adrien, E;Ahmed, A;Sanberg, PR;Bickford, PC;Gemma, C;Willing, AE;A single administration of human umbilical cord blood T cells produces long-lasting effects in the aging hippocampusAge (Dordrecht, Netherlands)23263793Cryopreserved HUCB MNC fraction and its component subfractions (CD3+, CD4+, CD8+, CD14+, and CD133+) were obtained from AllCells, LLC. HUCB cells were thawed in a 37 C SaveFull Paper
2013de Lichtervelde, L;Boitano, AE;Wang, Y;Krastel, P;Petersen, F;Cooke, MP;Schultz, PG;Eupalinilide E inhibits erythropoiesis and promotes the expansion of hematopoietic progenitor cellsACS chemical biology23441826Human mPB CD34 + cells were purified from fresh human leukophoresed mobilized peripheral blood (AllCells) using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following SaveFull Paper
2013Xiao, L;Hung, KC;Takahashi, TT;Joo, KI;Lim, M;Roberts, RW;Wang, P;Antibody-mimetic ligand selected by mRNA display targets DC-SIGN for dendritic cell-directed antigen deliveryACS chemical biology23427768Human PBMC-derived DCs were generated and purified as previously described, (46, 47) except that human PBMCs were from HLA-A2+ patients (AllCells, LLC.). Briefly, plastic-adherent cell fractions of human PBMCs were grown in RPMI 1640 with 10% heat-inFull Paper
2013Čokić, VP;Smith, RD;Biancotto, A;Noguchi, CT;Puri, RK;Schechter, AN;Globin gene expression in correlation with G protein-related genes during erythroid differentiationBMC genomics23425329isolation system (Miltenyi Biotec, Auburn, CA). Commercial FL- (Cambrex Bio Science, Inc., Walkersville, MD), CB-, BM- and granulocyte-colony stimulating factor (G-CSF) stimulated mobilized PB- (mPB, AllCells LLC, Berkeley, CA) derived CD34+ cells arFull Paper
2013Musiychuk, K;Sivalenka, R;Jaje, J;Bi, H;Flores, R;Shaw, B;Jones, RM;Golovina, T;Schnipper, J;Khandker, L;Sun, R;Li, C;Kang, L;Voskinarian-Berse, V;Zhang, X;Streatfield, S;Hambor, J;Abbot, S;Yusibov, V;Plant-produced human recombinant erythropoietic growth factors support erythroid differentiation in vitroStem cells and development23517237Umbilical cord blood-derived CD34 + cells were obtained from AllCells and maintained in the StemSpan Serum-Free Expansion Medium (StemCell Technologies) containing 1 μM SaveFull Paper
2013Ramalingam, S;London, V;Kandavelou, K;Cebotaru, L;Guggino, W;Civin, C;Chandrasegaran, S;Generation and genetic engineering of human induced pluripotent stem cells using designed zinc finger nucleasesStem cells and development22931452Human primary CBMNCs were purchased from Allcells, Inc. The frozen CBMNCs were thawed and then cultured in the serum free medium (SFM) supplemented with cytokines and a SaveFull Paper
2013Chen, J;Shi, ZD;Ji, X;Morales, J;Zhang, J;Kaur, N;Wang, S;Enhanced osteogenesis of human mesenchymal stem cells by periodic heat shock in self-assembling peptide hydrogelTissue engineering. Part A23072422Human bone marrow (BM) from the iliac crest of a 27-year-old donor was purchased (AllCells LLC, Berkeley, CA). Mononuclear cells were enriched and retrieved with RosetteSep MSC enrichment cocktail (StemCell Technologies, Vancouver, Canada) accordingFull Paper
2013Wei, N;Yu, Y;Joshi, V;Schmidt, T;Qian, F;Salem, AK;Stanford, C;Hong, L;Glucocorticoid receptor antagonist and siRNA prevent senescence of human bone marrow mesenchymal stromal cells in vitroCell and tissue research23963647Fresh human bone marrows, donated by total six healthy male and female donors from 22 to 43 years of age, were purchased from Allcells (Emeryville, CA). Mononuclear cells were isolated using density gradient centrifugation (Histopaque-1.077; Sigma, SFull Paper
2013Hutchinson, AT;Malik, A;Berkahn, MB;Agostino, M;To, J;Tacchi, JL;Djordjevic, SP;Turnbull, L;Whitchurch, CB;Edmundson, AB;Jones, DR;Raison, RL;Ramsland, PA;Formation of assemblies on cell membranes by secreted proteins: molecular studies of free λ light chain aggregates found on the surface of myeloma cellsThe Biochemical journal23822104We have described the presence of cell-membrane-associated κFLCs (free immunoglobulin light chains) on the surface of myeloma cells. Notably, the anti-κFLC mAb (monoclonal SaveFull Paper
2013Choudhary, R;Baturin, D;Fosmire, S;Freed, B;Porter, CC;Knockdown of HPRT for selection of genetically modified human hematopoietic progenitor cellsPloS one23555045Human umbilical cord blood samples unsuitable for clinical use were obtained from the ClinImmune Labs or were purchased from AllCells (Emeryville, CA). CD34 + cells were isolated SaveFull Paper
2013Song, L;Li, X;Jayandharan, GR;Wang, Y;Aslanidi, GV;Ling, C;Zhong, L;Gao, G;Yoder, MC;Ling, C;Tan, M;Srivastava, A;High-efficiency transduction of primary human hematopoietic stem cells and erythroid lineage-restricted expression by optimized AAV6 serotype vectors in vitro and in a murine xenograft model in vivoPloS one23516552fetal bovine serum (FBS; Sigma, St. Louis, MO), 100 µg/ml of penicillin and 100 U/ml of streptomycin (Invitrogen, Grand Island, NY). Human cord blood CD34+ cells and CD36+ cells were purchased from AllCells (AllCells Technologies, Emeryville, CA) andFull Paper
2013Wang, T;Choi, E;Monaco, MC;Campanac, E;Medynets, M;Do, T;Rao, P;Johnson, KR;Elkahloun, AG;Von Geldern, G;Johnson, T;Subramaniam, S;Hoffman, DA;Hoffman, D;Major, E;Nath, A;Derivation of neural stem cells from human adult peripheral CD34+ cells for an autologous model of neuroinflammationPloS one24303066marker to detect mesenchymal cells, as CD29 and CD271, were used to stain the isolated CD34+ to further asses their purity. CD34+ cell culture and transfection Cord blood CD34+ cells (purchased from Allcells, Emeryville, California) or adult CD34+ cFull Paper
2013Xiao, F;Kim, YC;Wen, H;Luo, J;Chen, P;Cowan, K;Wang, SM;The genome of polymorphonuclear neutrophils maintains normal coding sequencesPloS one24250807loid differentiation. Our study concludes that genomic DNA from myeloid lineage cells can be safely used in genetic studies. Methods Ethics Statement: The cells used for the study were obtained from AllCells LLC (http://www.allcells.com/, EmeryvilleFull Paper
2013Kornblau, SM;Qutub, A;Yao, H;York, H;Qiu, YH;Graber, D;Ravandi, F;Cortes, J;Andreeff, M;Zhang, N;Coombes, KR;Proteomic profiling identifies distinct protein patterns in acute myelogenous leukemia CD34+CD38- stem-like cellsPloS one24223100tcome. This population lack samples from the 10-15% of AML cases that are CD34 negative. As normal controls, 1x106 CD34+ cells were collected from 10 different unstimulated normal bone marrow donors (AllCells, Emeryville, CA http://www.allcells.com/)Full Paper
2013Chai, ZT;Kong, J;Zhu, XD;Zhang, YY;Lu, L;Zhou, JM;Wang, LR;Zhang, KZ;Zhang, QB;Ao, JY;Wang, M;Wu, WZ;Wang, L;Tang, ZY;Sun, HC;MicroRNA-26a inhibits angiogenesis by down-regulating VEGFA through the PIK3C2α/Akt/HIF-1α pathway in hepatocellular carcinomaPloS one24194905ecco’s modified Eagle’s medium (DMEM, Invitrogen, Carlsbad, CA) containing 10% fetal bovine serum (FBS) in a humidified incubator at 37°C with an atmosphere of 5% CO2. HUVECs were purchased from Allcells (Shanghai, China) and cultured in EndothelialFull Paper
2013Tarantal, AF;Lee, CC;Kukis, DL;Cherry, SR;Radiolabeling human peripheral blood stem cells for positron emission tomography (PET) imaging in young rhesus monkeysPloS one24098579CD34+ hPBSC were obtained from a commercial source (AllCells, Berkeley, CA) and cryopreserved using a controlled rate cryopreservation protocol in aliquots as previously reported [ SaveFull Paper
2013DeBoever, C;Reid, EG;Smith, EN;Wang, X;Dumaop, W;Harismendy, O;Carson, D;Richman, D;Masliah, E;Frazer, KA;Whole transcriptome sequencing enables discovery and analysis of viruses in archived primary central nervous system lymphomasPloS one24023918To serve as a positive control a cord blood sample was purchased from AllCells (Emeryville, CA). Peripheral blood mononuclear cells (PBMC) were extracted from peripheral blood SaveFull Paper
2013Dimicoli, S;Wei, Y;Bueso-Ramos, C;Yang, H;Dinardo, C;Jia, Y;Zheng, H;Fang, Z;Nguyen, M;Pierce, S;Chen, R;Wang, H;Wu, C;Garcia-Manero, G;Overexpression of the toll-like receptor (TLR) signaling adaptor MYD88, but lack of genetic mutation, in myelodysplastic syndromesPloS one23976989Bone marrows from healthy individuals were obtained from AllCells (Emeryville, CA). CD34+ cells were isolated from fresh bone marrow specimens of patients with MDS using SaveFull Paper
2013Dolley-Sonneville, PJ;Romeo, LE;Melkoumian, ZK;Synthetic surface for expansion of human mesenchymal stem cells in xeno-free, chemically defined culture conditionsPloS one23940553Prior to the initiation of the multi-passage study we evaluated several plating densities based on recommendation of the manufacturer of hBM-MSCs used in the study (AllCells) and SaveFull Paper
2013Holmberg, RC;Gindlesperger, A;Stokes, T;Lopez, D;Hyman, L;Freed, M;Belgrader, P;Harvey, J;Li, Z;Akonni TruTip(®) and Qiagen(®) methods for extraction of fetal circulating DNA--evaluation by real-time and digital PCRPloS one23936545’s Circulating Nucleic Acid Kit. In order to determine the comparative extraction efficiency of the two methods on a large set of male fetal specimens, 19 maternal plasma samples were collected by AllCells (Emeryville, CA) or StemExpress (PlacervillFull Paper
2013Hsiao, SC;Liu, H;Holstlaw, TA;Liu, C;Francis, CY;Francis, MB;Real time assays for quantifying cytotoxicity with single cell resolutionPloS one23826123To each well were added 180 µL of freshly drawn human blood (AllCells, Inc.). After 16 h of incubation at 37C, the samples were visualized directly. Because the target cells are all SaveFull Paper
2013Zhang, C;Wu, X;Zhang, M;Zhu, L;Zhao, R;Xu, D;Lin, Z;Liang, C;Chen, T;Chen, L;Ren, Y;Zhang, J;Qin, N;Zhang, X;Small molecule R1498 as a well-tolerated and orally active kinase inhibitor for hepatocellular carcinoma and gastric cancer treatment via targeting angiogenesis and mitosis pathwaysPloS one23755206nd by the vendors and subjected to in vitro assays between passages 8∼15, the cell lines for animal studies were between passages 5∼10. Human umbilical vein endothelial cell (HUVEC) obtained from Allcells (Emeryville, CA) was kept in EGM-2 (LONZA, AlFull Paper
2013Mendel, TA;Clabough, EB;Kao, DS;Demidova-Rice, TN;Durham, JT;Zotter, BC;Seaman, SA;Cronk, SM;Rakoczy, EP;Katz, AJ;Herman, IM;Peirce, SM;Yates, PA;Pericytes derived from adipose-derived stem cells protect against retinal vasculopathyPloS one23741506tient-derived lipoaspirate and further purified with serial passaging and sub-culturing, as we and others have previously shown [22]. Human bone marrow derived stem cells (hBMSCs) were purchased from Allcells (#MSC-001F). Bovine retinae and retinal mFull Paper
2013Su, RJ;Baylink, DJ;Neises, A;Kiroyan, JB;Meng, X;Payne, KJ;Tschudy-Seney, B;Duan, Y;Appleby, N;Kearns-Jonker, M;Gridley, DS;Wang, J;Lau, KH;Zhang, XB;Efficient generation of integration-free ips cells from human adult peripheral blood using BCL-XL together with Yamanaka factorsPloS one23704989ied by MACS as described previously. [40] MNCs were obtained by standard density gradient centrifugation with Ficoll-Hypaque (1.077 g/ml) at room temperature. Some PBMNCs were directly purchased from AllCells (Emeryville, CA). The age of male and femFull Paper
2013Evseenko, D;Latour, B;Richardson, W;Corselli, M;Sahaghian, A;Cardinal, S;Zhu, Y;Chan, R;Dunn, B;Crooks, GM;Lysophosphatidic acid mediates myeloid differentiation within the human bone marrow microenvironmentPloS one23696850emical analyses. The murine stromal cell lines MS5 and OP9 (American Type Culture Collection (ATCC, Rockville, MD, USA) or primary human bone marrow derived mesenchymal stromal cells (passage 2-3) (AllCells Inc, Emeryville CA) were seeded into chambeFull Paper
2013Melton, AC;Melrose, J;Alajoki, L;Privat, S;Cho, H;Brown, N;Plavec, AM;Nguyen, D;Johnston, ED;Yang, J;Polokoff, MA;Plavec, I;Berg, EL;O'Mahony, A;Regulation of IL-17A production is distinct from IL-17F in a primary human cell co-culture model of T cell-mediated B cell activationPloS one23505568Frozen vials of positively selected primary normal human CD19+ B cells and negatively selected CD4+ T cells were obtained from AllCells (Emeryville, CA). PBMC were isolated from SaveFull Paper
2013Angevine, K;Wuescher, L;Mensah-Osman, E;Loss of menin mediated by endothelial cells treated with CoPP is associated with increased maturation of adipocytesAdipocyte24052896, 10 mM l-glutamine, 0.75 units/mL heparin sulfate, 1 μg/mL hydrocortisone, 50 μg/mL ascorbic acid, and 2% fetal bovine serum. Bone marrow derived mesenchymal stem cells (MSCs) were purchased from AllCells and maintained at 37 °C and 5% CO2 in α-memFull Paper
2013Omana-Zapata, I;Oreizy, F;Mosqueda, F;Salemenik, M;Lin, A;Wen, JL;Varro, R;Lee-Haynes, L;Segurado, OG;Performance of a novel BD stem cell enumeration kit on two flow cytometry systemsInternational journal of laboratory hematology23190563Blood collection program and one frozen leukapheresis aliquot were enrolled for Linearity. Twenty-seven fresh leukapheresis aliquots from healthy mobilized adult donors were enrolled in the stability evaluation. All leukapheresis aliquots, fresh andFull Paper
2013Seki, K;Hisada, T;Kawata, T;Kamide, Y;Dobashi, K;Yamada, M;Mori, M;Okajima, F;Ishizuka, T;Oxidative stress potentially enhances FcεRI-mediated leukotriene C4 release dependent on the late-phase increase of intracellular glutathione in mast cellsBiochemical and biophysical research communications23998930Human cord blood CD34 + cells were purchased from ALLCELLS (Emeryville, CA) and cultured in complete medium consisting of RPMI1640 (Life Technologies, Carlsbad, CA) SaveFull Paper
2013Li, S;Garrett-Bakelman, FE;Akalin, A;Zumbo, P;Levine, R;To, BL;Lewis, ID;Brown, AL;D'Andrea, RJ;Melnick, A;Mason, CE;An optimized algorithm for detecting and annotating regional differential methylationBMC bioinformatics23735126Ten acute myeloid leukemia (AML) de-identified patient samples enriched for myeloblast cells and five normal bone marrow (NBM) samples (purchased from AllCells) were used in the SaveFull Paper
2013Ohkawa, S;Kamei, N;Kamei, G;Shi, M;Adachi, N;Deie, M;Ochi, M;Magnetic targeting of human peripheral blood CD133+ cells for skeletal muscle regenerationTissue engineering. Part C, Methods23298291)-mobilized human peripheral blood-derived-MNCs by using a direct immunomagnetic CD133 MicroBeads labeling and a magnetic cell sorting system, were purchased from AllCells( SaveFull Paper
2013Kim, J;Zanjani, ED;Jeanblanc, CM;Goodrich, AD;Hematti, P;Generation of CD34+ cells from human embryonic stem cells using a clinically applicable methodology and engraftment in the fetal sheep modelExperimental hematology23612043For generation of BM CD14 + cell-derived macrophages, CD14 + cells were isolated from BM aspirates of normal donors (AllCells) and harvested after 1 week (n = 6). For generation of SaveFull Paper
2013Cherukuri, A;Patton, K;Gasser, RA;Zuo, F;Woo, J;Esser, MT;Tang, RS;Adults 65 years old and older have reduced numbers of functional memory T cells to respiratory syncytial virus fusion proteinClinical and vaccine immunology : CVI23239796A pooled human reference plasma standard (AllCells, LLC, Emeryville, CA) was included on each plate for comparison, and all plates were incubated for 1 h. A SulfoTag-labeled goat SaveFull Paper
2013Kim, DH;Liu, J;Bhat, S;Benedict, G;Lecka-Czernik, B;Peterson, SJ;Ebraheim, NA;Heck, BE;Peroxisome proliferator-activated receptor delta agonist attenuates nicotine suppression effect on human mesenchymal stem cell-derived osteogenesis and involves increased expression of heme oxygenase-1Journal of bone and mineral metabolism22945906Frozen bone marrow mononuclear cells were purchased from AllCells (Emeryville, CA, USA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential SaveFull Paper
2013Li, L;Wang, Y;Chen, J;Cheng, B;Hu, J;Zhou, Y;Gao, X;Gao, L;Mei, X;Sun, M;Zhang, Z;Song, H;An Engineered Arginase FC Protein Inhibits Tumor Growth In Vitro and In VivoEvidence-based complementary and alternative medicine : eCAM23737831umbilical cord vein were purchased from Allcells Inc. A confluent monolayer of synchronized HUVECS was scraped with a razor blade as described in [14]. After wounding, the cells SaveFull Paper
2013Tummala, S;Titus, M;Wilson, L;Wang, C;Ciatto, C;Thill, G;Foster, D;Li, C;Szabo, Z;Guttman, A;Bettencourt, B;Jayaraman, M;Deroot, J;Kocisko, D;Pollard, S;Charisse, K;Kuchimanchi, S;Hinkle, G;Milstein, S;Meyers, R;Wu, SL;Karger, BL;Rossomando, A;Evaluation of exogenous siRNA addition as a metabolic engineering tool for modifying biopharmaceuticalsBiotechnology progress2317273512K media containing 10% fetal bovine serum. Fresh human normal PBMCs were purchased from AllCells (Emeryville, CA). Approximately 1 ×105 Jeko-1 cells were pre-incubated with RNAi-treated or control antibodies at varying concentrations ranging from 0Full Paper
2013Rocha, LR;Veiga, DF;e Oliveira, PR;Song, EH;Ferreira, LM;Health Service Quality Scale: Brazilian Portuguese translation, reliability and validityBMC health services research23327598Business School, Avinguda de Pearson, 21, 08034, Barcelona, Spain Find articles by Paulo Rocha e Oliveira [https://pubmed.ncbi.nlm.nih.gov/?term=e%20Oliveira%20PR%5BAuthor%5D] ELAINE HORIBE SONG 4AllCells LLC, 5858 Horton Street, Emeryville, CA, 9Full Paper
2013Ross, KM;McDonald-Jones, G;Miller, GE;Oxytocin does not attenuate the ex vivo production of inflammatory cytokines by lipopolysaccharide-activated monocytes and macrophages from healthy male and female donorsNeuroimmunomodulation23899661For MP experiments, we purchased MO-derived, in vitro matured MPs from a cell supplier (AllCells, Emeryville, CA). All MPs used here were matured from a single donor's peripheral Save;Background: Oxytocin (OT) is a neuropeptide shown to attenuate iFull Paper
2013Wu, Y;Yang, Y;Yang, P;Gu, Y;Zhao, Z;Tan, L;Zhao, L;Tang, T;Li, Y;The osteogenic differentiation of PDLSCs is mediated through MEK/ERK and p38 MAPK signalling under hypoxiaArchives of oral biology23806288The bone marrow stromal stem cells (BMSSCs) used as controls were obtained from a commercially available resource (AllCells LLC, Berkeley, CA, USA). SaveFull Paper
2013Miller, P;Stabilization of memory States by stochastic facilitating synapsesJournal of mathematical neuroscience24314108S is a scaled version of s, accounting for the totalfeedback conductance, S = W s , where W is the sum of connection strengths of allcells and held fixed when N is varied. SaveFull Paper
2013Zhan, H;Cardozo, C;Yu, W;Wang, A;Moliterno, AR;Dang, CV;Spivak, JL;MicroRNA deregulation in polycythemia vera and essential thrombocythemia patientsBlood cells, molecules & diseases23265742the peripheral blood CD34+ cells were 95% positive both for CD34 and CD38, were dimly positive for CD117, were CD41 negative, and were primarily in G0/G1 of the cell cycle [31]. Peripheral blood CD34+ cells from healthy controls were purchased from AFull Paper
2013Li, H;Xie, W;Gore, ER;Montoute, MN;Bee, WT;Zappacosta, F;Zeng, X;Wu, Z;Kallal, L;Ames, RS;Pope, AJ;Benowitz, A;Erickson-Miller, CL;Development of phenotypic screening assays for γ-globin induction using primary human bone marrow day 7 erythroid progenitor cellsJournal of biomolecular screening24163393All cryopreserved human bone marrow CD34 + cells used here were obtained from AllCells (Emeryville, CA) and were generally from different donors. The CD34 + cells were cultured SaveFull Paper
2013Schwimmer, LJ;Huang, B;Giang, H;Cotter, RL;Chemla-Vogel, DS;Dy, FV;Tam, EM;Zhang, F;Toy, P;Bohmann, DJ;Watson, SR;Beaber, JW;Reddy, N;Kuan, HF;Bedinger, DH;Rondon, IJ;Discovery of diverse and functional antibodies from large human repertoire antibody librariesJournal of immunological methods23454004The source RNA and cDNA used to amplify the V-genes was purchased from AllCells and Cureline. The E. coli strain TG1 (Lucigen) was used for all molecular cloning, phage SaveFull Paper
2013Rosa, M;Pragasam, P;Saremian, J;Aoalin, A;Graf, W;Mohammadi, A;The unsatisfactory ThinPrep® Pap Test™: analysis of technical aspects, most common causes, and recommendations for improvementDiagnostic cytopathology22833404Fourth slide displays "halo effect" in which allcells are located at the periphery of the slide. This represents a technical preparation problem that can be resolved in the majority of cases SaveFull Paper
2013Vainer, ED;Meir, K;Furman, M;Semenenko, I;Konikoff, F;Vainer, GW;Characterization of novel CD55 isoforms expression in normal and neoplastic tissuesTissue antigens23692281RNA was obtained from Ambion (Austin, TX), Asterand (Detroit, MI), AllCells (Emeryville, CA), Genomics Collaborative (Seracare; Cambridge, MA), Biochain (Newark, CA) and Ichilov medical center (Tel-aviv, Israel). CRC cell lines: colo205, DLD-1, HCT11Full Paper
2013Muluk, NB;Arikan, OK;Atasoy, P;Kiliç, R;Yalçinozan, ET;Inducible nitric oxide synthase (iNOS) in sinonasal polyp pathogenesisB-ENT24273952the allcells group of inflammatory cells, there were PMNCs, MNCs, and fibroblasts. Higher iNOS expression in the allcells Thus, iNOS expression in the allcells group was mainly due to SaveFull Paper
2013Wagner, LA;Wang, S;Wayner, EA;Christensen, C;Perkins, SJ;Ward, GW;Weiss, RB;Dunn, DM;Redd, MJ;Spangrude, GJ;Gleich, GJ;Developing and mature human granulocytes express ELP 6 in the cytoplasmHuman antibodies24284306R&D Systems) or 2 U/ml epoietin-α [18]. Collection of de-identified UCMC was approved by the University of Utah’s Institutional Review Board. Bone marrow mononuclear cells (BMMC) were purchased from AllCells (Emeryville, CA)Full Paper
2013Köllmer, M;Buhrman, JS;Zhang, Y;Gemeinhart, RA;Markers Are Shared Between Adipogenic and Osteogenic Differentiated Mesenchymal Stem CellsJournal of developmental biology and tissue engineering24013643Human bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque TM PLUS solution followed by cell SaveFull Paper
2013Hu, K;Slukvin, I;Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectorsMethods in molecular biology (Clifton, N.J.)23546755Human cord blood mononuclear cells (AllCells, CA, USA). 12. Human bone marrow mononuclear cells (AllCells, CA, USA). 13. Human bone marrow cells from patient with CML (AllCells, CA, USA). 14. Human cord blood CD34+ cells (AllCells, CA, USA). 15.Full Paper
2013Chu, EM;Tai, DC;Beer, JL;Hill, JS;Macrophage heterogeneity and cholesterol homeostasis: classically-activated macrophages are associated with reduced cholesterol accumulation following treatment with oxidized LDLBiochimica et biophysica acta23142249CD14 + human peripheral blood monocytes (AllCells) were cultured in RPMI 1640 complete media (Hyclone) containing 10% FBS (Hyclone), 2% sodium bicarbonate, 1% sodium SaveFull Paper
2013Arora, M;Cell Culture Media: A ReviewMaterials and Methodsary cells. CELLS MEDIA Endothelial cells Human Endothelial-SFM (Life Technologies), Endothelial Basal Media, EndoGRO-LS Complete Media Kit (MilliporeSigma), HUVEC Basal Medium CB HUVEC (AllCells), Endothelial Cell Medium (ScienCell)Full Paper
2013Mirshokraie, S;Mesbah, A;Pattabiraman, K;Efficient JavaScript Mutation Testing2013 IEEE Sixth International Conference on Software Testing, Verification and Validationfunction calls setup to set the dimension of all DOM elements with a class name of allCells; Assuming that the number of DOM elements with class name allCells is 9 (line 3 in Figure 1), SaveFull Paper
2013Fox, S;Adachi, T;Stillwell, W;A QUINONE-ASSISTED PHOTOFORMATION OF ENERGY-RICH CHEMICAL BONDSSolar Energy International ProgressIn severalconferences , biochemists , chemicalengineers , andothershavediscusse dhow to industrializephotosyn thesis and how to industrializethe ATP cyclethatoperates in allcells . T SaveFull Paper
2013Margules, D;Flynn, J;Cooper, C;CALCITONIN-LIKE PEPTIDE IN PRO-OPIOCORTIN: FUNCTIONAL ASPECTSAdvances in Endogenous and Exogenous Opioidscells of the anterior pituitary and in allcells of the intermediate pituitary . EXCESS IN OBESE cells of the anterior pituitary and in allcells of the intermediate pituitary . EXCESS IN OBESE SaveFull Paper
2013Brodie, G;CONSTRUCTED WETLANDS FOR TREATING ACID DRAINAGE AT TENNESSEE VALLEY AUTHORITY COAL FACILITIESConstructed Wetlands in Water Pollution ControlS tagedtreatmentusingse veralcells may providea more efficientsystemsincec hemical and hydraulicoverloading of allcells would be minimized. Acid drainage treatment Water depth SaveFull Paper
2013Masuda, H;Asahara, T;Isolation of Colony-Forming Endothelial Progenitor CellsManual of Research Techniques in Cardiovascular Medicineapproval of the institutional ethical committees of the Cord Blood and/or Clinical Investigation Committee. Alternatively, isolated MNCs, CD34+ , or CD133+ cells in UCB, PB, or BM can be purchased (e.g. ALLCELLSTM: http://www.allcells.com)Full Paper
2013Drew, H;Analysis of Macrophage-ThesisLPS, bovine CS and bovine aggrecan were purchased from SigmaAldrich (St. Louis, MO). Human monocytes (AllCells, LCC, Emeryville, CA) were cultured in RPMI-1640 with 10% fetal bovine serum. Bovine keratan sulfate proteoglycan (KSPG) was purchased fFull Paper
2013Adams, GN;Prolylcarboxypeptidase protects from vascular dysfunction and promotes vascular repairThesisHUVEC were purchased from AllCells (Emeryville, CA) and grown on gelatin coated plates in CSC media (Cells Systems; Kirkland, WA). Cells were grown to near confluence in 6-well plates and transfected with Lipofectamine 2000 reagent (Invitrogen) in OPFull Paper
2013Patel, VM;Investigating apoptosis pathway in chronic lymphocytic leukemia: stromal influence and therapeutic activationThesisAll subjects signed written informed consent forms in accordance with the Declaration of Helsinki, and the laboratory protocols were approved by the institutional review board (IRB) at the University of Texas MD Anderson Cancer Center. Normal B‐lymphFull Paper
2013Yi, S;Green Manufacturing of Nanoparticles for Biomedical ApplicationsThesisSplenocytes, derived from C57BL/6 mice, were purchased from the Allcells Company (Emeryville, CA). HEPES, 1, 9-dimethyl-methylene blue (DMMB), chondroitin sulfate (CS), Sephadex G75, DEAE-cellulose and phosphate buffered saline (PBS) were purchased fFull Paper
2013Adams, KJ;Redirected T cell activity by high affinity TCR-ANTI-CD3 bispecific candidate therapeuticsThesis2.2.2.3 Frozen peripheral blood mononuclear cells (PBMC) derived from multiple myeloma patients PBMC derived from multiple myeloma patients were purchased from AllCells as frozen aliquots, and were thawed and used on the day of the assayFull Paper
2013PURWANTI, YIDA;CANCER IMMUNOTHERAPY: TARGETED CELLULAR VEHICLE-MEDIATED IMMUNOGENE THERAPY AND DENDRITIC CELL-BASED VACCINEThesisDCs and naïve T cells were derived from human peripheral blood mononuclear cells (PBMCs), both HLA-A2 positive and negative (AllCells, Stemcell Technologies Inc) based on previous studies 178. To generate DCs, 1×108 human PBMCs were seeded onto a T-1Full Paper
2013Kalim, KW;The role of immunoproteasome subunit LMP7 in modulating T-helper cell differentiation and progression of autoimmune diseasesThesiscells were obtained from ATCC (Manassas, VA) and cultured in media recommended by the supplier. Human peripheral blood mononuclear cells (PBMC) from normal healthy volunteers were purchased from AllCells (Emeryville, CA) and those from individuals wiFull Paper
2013Suknuntha, K;An Induced Pluripotent Stem Cell-Based Platform for In Vitro Study of Chronic Myeloid Leukemia Stem Cell Development and Drug ResistanceThesiscells from CML patients in the chronic phase were purchased commercially (AllCells or in the chronic phase were purchased commercially (AllCells or Applied StemCells), or obtained SaveFull Paper
2013O'Huallachain, ME;Genomic Variation in Somatic Human TissuesThesisfor a complexrearrangement in the PMP22 gene in an unaffected parent of a patient affect byCharcot-Marie-Tooth disease who had the same rearrangement throughout allcells. SaveFull Paper
2013Bhat, S;Impact of nicotine and PPARδ-agonist on human mesenchymal stem cellsThesisAn overview of all experiments performed in this thesis is provided on the previous page (see Figure 7). The overview includes type of experiment, treatments and assay/staining procedures conducted. Frozen (in 10%DMSO) bone marrow mononuclear cells (Full Paper
2013Kadam, S;Global Landscape of DNA Methylation and Gene Expression in Hematopoiesis and Monocytic DifferentiationThesisTable 1: Information about the samples obtained from AllCells Inc; age, sex, ethnicity, lot number, catalog number, and location of cell (Bone Marrow and Peripheral Blood). RNA Expression Analysis in the Hematopoetic Lineage Even though DNA methylatiFull Paper
2013Kohn, LA;Identification and Characterization of the Earliest Stages of Human Lymphoid CommitmentThesisTissue Core of the University of California, Los Angeles, Cincinnati Children’s Hospital, or AllCells according to guidelines approved by Institutional Review Board of the University of California, Los Angeles. Samples were enriched for CD34+ cellsFull Paper
2013Haworth, KG;Molecular Characterization of the HIV-1 Vpu Protein and Its Role in Antagonizing the Cellular Restriction Factor BST-2/Tetherin Both In Vitro and In VivoThesisHuman peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA), depleted of CD8+ T cells using a positive selection CD8 microbead isolation kit (Miltenyi Biotech, Gladbach, Germany), and cultured in supplemented R10 mediFull Paper
2013Cullimore, M;Establishing a role for IL-27 in the amelioration of MS and EAEThesisWhole venous blood was collected in EDTA-treated tubes from healthy donors after their informed consent. PBMCs were isolated using FiColl-Paque Plus™ density centrifugation while cord blood cells were obtained from AllCells. CD4+ /CD14+ cells were puFull Paper
2013Johnston, JM;Optimization of a Lentiviral Vector for use in an HSC Gene Therapy Protocol for Hemophilia AThesisCD34+ cells were purchased from AllCells (Emeryville, CA). Prior to arrival, CD34+ cells were mobilized from human bone marrow and isolated from the peripheral blood of 3 individual subjects. Flow cytometry was used to assess the purity of the sampleFull Paper
2013Xiao, L;Dendritic cell-specific vaccine utilizing antibody-mimetic ligand and lentivector systemThesisHuman PBMC-derived DCs were generated and purified as previously described,(Romani and others 1994; Sallusto and others 1994) except that human PBMCs were from HLA-A2+ patients (AllCells, LLC.). Briefly, plastic-adherent cell fractions of human PBMCsFull Paper
2013Ji, T;Lin, C;Krill, LS;Eskander, R;Guo, Y;Zi, X;Hoang, BH;Flavokawain B, a kava chalcone, inhibits growth of human osteosarcoma cells through G2/M cell cycle arrest and apoptosisMolecular cancer23764122number of 2×104 cells were mixed with FKB or Adriamycin at concentration of 8.8 nM/ml, 17.6 nM/ml and 26.4 nM/ml, respectively. The mixture was cultured in 1 ml ColonyGel 1201 Mouse Base Medium (Reachbio) using a 6-well plate under standard cultureFull Paper
2013Lee, YT;de Vasconcellos, JF;Yuan, J;Byrnes, C;Noh, SJ;Meier, ER;Kim, KS;Rabel, A;Kaushal, M;Muljo, SA;Miller, JL;LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivoBlood23798711All related studies were performed after human subjects review and National Institutes of Health Institutional Review Board approval. These studies were conducted in accordance with the Declaration of Helsinki. Human cord blood CD34+ cells were obtaiFull Paper
2013Brandao, LN;Winges, A;Christoph, S;Sather, S;Migdall-Wilson, J;Schlegel, J;McGranahan, A;Gao, D;Liang, X;Deryckere, D;Graham, DK;Inhibition of MerTK increases chemosensitivity and decreases oncogenic potential in T-cell acute lymphoblastic leukemiaBlood cancer journal23353780w cytometer and CXP data analysis software (Beckman Coulter). Methylcellulose assay Five hundred Jurkat parental and shRNA derivative cells were plated in methylcellulose-based medium (Cat no. 1101, Reachbio, Seattle, WA, USA) according to manufactuFull Paper
2013Grandi, FC;Rosser, JM;An, W;LINE-1-derived poly(A) microsatellites undergo rapid shortening and create somatic and germline mosaicism in miceMolecular biology and evolution23125228Cell and viability counts were performed using glacial acetic acid and trypan blue. Bone marrow cells were plated in ColonyGEL Complete Medium Without Epo (ReachBio LLC) at a density of 2 × 104 or 1 × 104 cells in 35 mm cell culture dishes and culturFull Paper
2013Ulyanova, T;Jiang, Y;Padilla, SM;Papayannopoulou, T;Erythroid cells generated in the absence of specific β1-integrin heterodimers accumulate reactive oxygen species at homeostasis and are unable to mount effective antioxidant defensesHaematologica23812936CFU-C assays To quantify committed progenitors of all lineages, 50,000 BM cells, 500,000 splenic cells or 50-250 microliters of peripheral blood were cultured in semi-solid methylcellulose medium supplemented with cytokines (ReachBio) for seven daysFull Paper
2013Zeiger, AS;Hinton, B;Van Vliet, KJ;Why the dish makes a difference: quantitative comparison of polystyrene culture surfacesActa biomaterialia23454055Human bone-marrow-derived mesenchymal stromal or “stem” cells (hMSCs; ReachBio Seattle, WA) were expanded until passages 4-6, and plated onto P35 TCPS dishes at low density (∼5000 cells cm−2) to maintain subconfluent culture conditionsFull Paper
2013Lee-Sherick, AB;Eisenman, KM;Sather, S;McGranahan, A;Armistead, PM;McGary, CS;Hunsucker, SA;Schlegel, J;Martinson, H;Cannon, C;Keating, AK;Earp, HS;Liang, X;DeRyckere, D;Graham, DK;Aberrant Mer receptor tyrosine kinase expression contributes to leukemogenesis in acute myeloid leukemiaOncogene23474756For methylcellulose colony forming assays, cells were plated in triplicate at a density of 500 cells/plate in Colony Gel Human Base Medium methylcellulose (Reach Bio, Seattle, WA, USA) with RPMI media containing 10% FBS added per manufacturer instrucFull Paper
2013Rossetto, CC;Tarrant-Elorza, M;Pari, GS;Cis and trans acting factors involved in human cytomegalovirus experimental and natural latent infection of CD14 (+) monocytes and CD34 (+) cellsPLoS pathogens23717203d, 10 ng/ml TPO, and 10 ng/ml TNF for three days, followed by the addition of 50 ng/ml lipopolysaccharide (LPS) (Sigma-Aldrich) for an additional four days. Human CD14 (+) cells purchased (Lonza and ReachBio) or isolated from cord blood were maintaiFull Paper
2013Christoph, S;Deryckere, D;Schlegel, J;Frazer, JK;Batchelor, LA;Trakhimets, AY;Sather, S;Hunter, DM;Cummings, CT;Liu, J;Yang, C;Kireev, D;Simpson, C;Norris-Drouin, J;Hull-Ryde, EA;Janzen, WP;Johnson, GL;Wang, X;Frye, SV;Earp, HS;Graham, DK;UNC569, a novel small-molecule mer inhibitor with efficacy against acute lymphoblastic leukemia in vitro and in vivoMolecular cancer therapeutics23997116Jurkat or 697 cells were plated in methylcellulose-based medium (#1101; ReachBio) containing UNC569 or DMSO control according to the manufacturer's protocol (500 cells/mL SaveFull Paper
2013Bausch, E;Kohlhof, H;Hamm, S;Krauss, R;Baumgartner, R;Sironi, L;A novel microtubule inhibitor 4SC-207 with anti-proliferative activity in taxane-resistant cellsPloS one24324550Mouse femoral bone marrow cells derived from an 8 week old Balb/c mouse were qualified at ReachBio (in terms of progenitor frequency), and stored at -152C. Cells were prepared as Save;the absorbance at time zero (drug addition), Ti the absorbance aFull Paper
2013Weerasinghe, P;Hallock, S;Brown, RE;Loose, DS;Buja, LM;A model for cardiomyocyte cell death: insights into mechanisms of oncosisExperimental and molecular pathology22609242)-derived cardiomyocytes were purchased from Reach Bio LLC (Seattle, WA) and neonatal Sanguinarine was subsequently diluted with cardiomyocyte growth media (Reach Bio LLC, SaveFull Paper
2013Maloney, JM;Lehnhardt, E;Long, AF;Van Vliet, KJ;Mechanical fluidity of fully suspended biological cellsBiophysical journal24138852Primary adult human mesenchymal stem cells (hMSCs) were isolated from the bone marrow of four adult donors via Stem Cell Technologies (Vancouver, BC, Canada), ReachBio (Seattle, WA), or Lonza Group (Basel, Switzerland)Full Paper
2013Whitfield, MJ;Lee, WC;Van Vliet, KJ;Onset of heterogeneity in culture-expanded bone marrow stromal cellsStem cell research24103495BMSCs used for long-term imaging were purchased from ReachBio, and received at passage 1 (p1). Cells were seeded at ~ 2000 cells/cm 2 on tissue culture-treated polystyrene (BD SaveFull Paper
2013Pierce, T;Keeping Piracy to a MinimumAppreneurTwo assay platforms, specifically designed for ADCs, have been developed by ReachBio as a result of increasing demand by industry for ways to assess the potential toxicity of these SaveFull Paper
2013Kennedy, JP;A cellularized biomaterial model of cardiac fibroblasts for evaluation of fibroblast induced changes in stiffnessThesisSingle donor human mesenchymal stem cells (MSC; ReachBio Seattle, WA) were passaged 2-3, and expression of CD29, CD73, CD105 CD34, and CD45 surface markers was evaluated by FACS (flow cytometry performed by manufacturer). UFull Paper
2013Rosser, JM;Investigating DNA methylation dynamics with transgenic LINE-1 mouse modelsThesisCell and viability counts were performed using glacial acetic acid and trypan blue. Bone marrow cells were plated in ColonyGEL Complete Medium Without Epo (ReachBio LLC) at a densityFull Paper
2012Xu, B;Ionita-Laza, I;Roos, JL;Boone, B;Woodrick, S;Sun, Y;Levy, S;Gogos, JA;Karayiorgou, M;De novo gene mutations highlight patterns of genetic and neural complexity in schizophreniaNat. Genet.23042115We are enormously grateful to all the families who participated in this research. We thank H. Pretorius and nursing sisters R. van Wyk, C. Botha and H. van den Berg for their assistance with subject recruitment, family history assessments and diagnosFull Paper
2012Yang, J;Ramos, E;Corces, VG;The BEAF-32 insulator coordinates genome organization and function during the evolution of Drosophila speciesGenome Res.22895281on a Branson Sonifier 250 with output control set at 1.5. Libraries were prepared with the Illumina TruSeq DNA Sample Preparation Kit and sequenced at the HudsonAlpha Institute for Biotechnology.Full Paper
2012Van Bortle, K;Ramos, E;Takenaka, N;Yang, J;Wahi, JE;Corces, VG;Drosophila CTCF tandemly aligns with other insulator proteins at the borders of H3K27me3 domainsGenome Res.22722341Ligated ChIP samples were PCR-amplified using Illumina primers and Phusion DNA polymerase (NEB Cat# F-530L) and size selected for 200–300 bp by gel extraction. ChIP libraries were sequenced at the HudsonAlpha Institute for Biotechnology, using an IllFull Paper
2012Kellner, WA;Ramos, E;Van Bortle, K;Takenaka, N;Corces, VG;Genome-wide phosphoacetylation of histone H3 at Drosophila enhancers and promotersGenome Res.22508764We thank Mattias Mannervik for the gift of CBP antibody and members of the Corces Lab for invaluable discussions. We also thank The Genomic Services Lab at the HudsonAlpha Institute for Biotechnology for their help in performing Illumina sequencing oFull Paper
2012Higgins, G;Roper, KM;Watson, IJ;Blackhall, FH;Rom, WN;Pass, HI;Ainscough, JF;Coverley, D;Variant Ciz1 is a circulating biomarker for early-stage lung cancerProc. Natl. Acad. Sci. U.S.A.23074256... anti-rabbit Alexa Fluor 488 (Invitrogen, Folio Bioscience). Subcellular fractionation was carried out as described (21). For immunohistochemistry, 2B was applied to SCLC tissue microarray ARY-HH0117 (Folio Biosciences) and visualized using UltravFull Paper
2012Fabbri, M;Paone, A;Calore, F;Galli, R;Gaudio, E;Santhanam, R;Lovat, F;Fadda, P;Mao, C;Nuovo, GJ;Zanesi, N;Crawford, M;Ozer, GH;Wernicke, D;Alder, H;Caligiuri, MA;Nana-Sinkam, P;Perrotti, D;Croce, CM;MicroRNAs bind to Toll-like receptors to induce prometastatic inflammatory responseProc. Natl. Acad. Sci. U.S.A.22753494We thank Phylogeny, Inc. for performing in situ hybridization in its entirety and locked nucleic acid in situ hybridization experiments and for supporting these experiment; Dr. Cecilia FernandezCymering and Dr. Stefano Volinia for supervision of statFull Paper
2012Ren, H;Tan, ZP;Zhu, X;Crosby, K;Haack, H;Ren, JM;Beausoleil, S;Moritz, A;Innocenti, G;Rush, J;Zhang, Y;Zhou, XM;Gu, TL;Yang, YF;Comb, MJ;Identification of anaplastic lymphoma kinase as a potential therapeutic target in ovarian cancerCancer Res.22570254Histologic typing and grading were carried out according to International Federation of Gynecology and Obstetrics (FIGO) guidelines. General pathologic information of the 69 ovarian tumor patients is listed in Supplementary Table S1. FFPE ovarian tumFull Paper
2012Polisetty, RV;Gautam, P;Sharma, R;Harsha, HC;Nair, SC;Gupta, MK;Uppin, MS;Challa, S;Puligopu, AK;Ankathi, P;Purohit, AK;Chandak, GR;Pandey, A;Sirdeshmukh, R;LC-MS/MS analysis of differentially expressed glioblastoma membrane proteome reveals altered calcium signaling and other protein groups of regulatory functionsMol. Cell Proteomics22219345... tion [http://www.mcponline.org/cgi/content/full/M111.013565/DC1]. IMMUNOHISTOCHEMISTRY Formalin-fixed paraffin-embedded individual GBM tissue sections or commercially available tissue microarrays (Folio Biosciences, Columbus, OH) with 35 GBM tFull Paper
2012Gonzalez de Castro, D;Angulo, B;Gomez, B;Mair, D;Martinez, R;Suarez-Gauthier, A;Shieh, F;Velez, M;Brophy, VH;Lawrence, HJ;Lopez-Rios, F;A comparison of three methods for detecting KRAS mutations in formalin-fixed colorectal cancer specimensBr. J. Cancer22713664Materials: FFPET specimens from CRC tumours were purchased from US commercial vendors: Discovery Life Sciences, Inc. (Los Osos, CA, USA); BioServe (Beltsville, MD, USA); ProteoGenex (Culver City, CA, USA); CureLine, IncFull Paper
2012Lenehan, PF;Boardman, LA;Riegert-Johnson, D;De Petris, G;Fry, DW;Ohrnberger, J;Heyman, ER;Gerard, B;Almal, AA;Worzel, WP;Generation and external validation of a tumor-derived 5-gene prognostic signature for recurrence of lymph node-negative, invasive colorectal carcinomaCancer22605513(South San Francisco, Calif), Mayo Clinic (Rochester, Minn; Jacksonville, Fla; and Scottsdale, Ariz), Folio Biosciences (Columbus, Ohio), Trans‐Hit Biomarkers, Inc. (Montreal, Quebec, Canada), BioServe (Laurel, Md), and Amp‐Tec (Hamburg, Germany)Full Paper
2012Arrington, CB;Bleyl, SB;Matsunami, N;Bonnell, GD;Otterud, BE;Nielsen, DC;Stevens, J;Levy, S;Leppert, MF;Bowles, NE;Exome analysis of a family with pleiotropic congenital heart diseaseCirc Cardiovasc Genet22337856Exome sequencing (library construction, capture and sequencing) was carried out on 2 DNA samples (from patients III.3 and III.7) at the genomic service laboratory at HudsonAlpha Institute for Biotechnology (Huntsville, Alabama). Briefly, 3 to 5 µg ofFull Paper
2012Dahmen, S;Haenni, M;Madec, JY;IncI1/ST3 plasmids contribute to the dissemination of the blaCTX-M-1 gene in Escherichia coli from several animal species in FranceJ. Antimicrob. Chemother.22872449Characteristics of the eight CTX-M-1-producing E. coli isolates described in this study Strain Year District Animal Pathology b-Lactamases Additional resistancesa Phylogeny CTX-M-1 plasmid Inc group sequence type size (kb) 16170 2006 76 dog diarrhoeaFull Paper
2012Fedyk, ER;Wyant, T;Yang, LL;Csizmadia, V;Burke, K;Yang, H;Kadambi, VJ;Exclusive antagonism of the α4 β7 integrin by vedolizumab confirms the gut-selectivity of this pathway in primatesInflamm. Bowel Dis.22419649Immunohistochemistry detecting the β7 integrin was conducted using a sheep antihuman β7 polyclonal antibody (R&D Systems, Minneapolis, MN) that does not compete with vedolizumab for binding to the α4β7 integrin expressed by leukocytes (data not shownFull Paper
2012Jenny, MJ;Aluru, N;Hahn, ME;Effects of short-term exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin on microRNA expression in zebrafish embryosToxicol. Appl. Pharmacol.22921993The same total RNA used for the array hybridizations was also used for high-throughput sequencing using the SOLiD technology. Prior to sequencing, the quality of the RNA and presence of the microRNA fraction was confirmed with the Agilent Small RNA kFull Paper
2012Liu, S;Zhang, Y;Zhou, Z;Waldbieser, G;Sun, F;Lu, J;Zhang, J;Jiang, Y;Zhang, H;Wang, X;Rajendran, KV;Khoo, L;Kucuktas, H;Peatman, E;Liu, Z;Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygoteBMC Genomics23127152;... urer's protocol as in previous study [5]. Equal amount of total RNA from each tissue was combined and sent out for commercial sequencing. Illumina sequencing Sequencing was conducted commercially in HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2012Rokyta, DR;Lemmon, AR;Margres, MJ;Aronow, K;The venom-gland transcriptome of the eastern diamondback rattlesnake (Crotalus adamanteus)BMC Genomics23025625Sequencing and nonnormalized cDNA library preparation were performed by the HudsonAlpha Institute for Biotechnology Genomic Services Laboratory (http://www.hudsonalpha.org/gsl/). Transcriptome sequencing was performed essentially as described by MortFull Paper
2012Sun, F;Peatman, E;Li, C;Liu, S;Jiang, Y;Zhou, Z;Liu, Z;Transcriptomic signatures of attachment, NF-κB suppression and IFN stimulation in the catfish gill following columnaris bacterial infectionDev. Comp. Immunol.; for use in RNA-seq. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA) as previously described by Li et al. (2012). cDNA libraries were prepared withFull Paper
2012Li, C;Zhang, Y;Wang, R;Lu, J;Nandi, S;Mohanty, S;Terhune, J;Liu, Z;Peatman, E;RNA-seq analysis of mucosal immune responses reveals signatures of intestinal barrier disruption and pathogen entry following Edwardsiella ictaluri infection in channel catfish, Ictalurus punctatusFish Shellfish Immunol.22366064; replicate control pool was formed for use in RNA-seq. RNA-seq library preparation and sequencing was carried out by HudsonAlpha Genomic Services Lab (Huntsville, AL, USA). cDNA libraries were prepared with 2.14-3.25 ug ofFull Paper
2012Nguyen, TH;Weber, W;Havari, E;Connors, T;Bagley, RG;McLaren, R;Nambiar, PR;Madden, SL;Teicher, BA;Roberts, B;Kaplan, J;Shankara, S;Expression of TMPRSS4 in non-small cell lung cancer and its modulation by hypoxiaInt. J. Oncol.22692880Immunohistochemistry. A tissue array containing 101 formalin-fixed, paraffin-embedded tissue cores of lung SCC, adenocarcinomas, and normal non-neoplastic lung tissues from multiple donors was used (Phylogeny Inc., Columbus, OH)Full Paper
2012Gurudatta, BV;Ramos, E;Corces, VG;The BEAF insulator regulates genes involved in cell polarity and neoplastic growthDev. Biol.22743648ChIP libraries were sequenced at the HudsonAlpha Institute for Biotechnology, using an Illumina HiSeq system. Sequences were mapped to the dm3 genome with Bowtie 0.12.3 (Langmead et al., 2009) using default settings. Peaks were then called with MACSFull Paper
2012Lee, S;Brophy, VH;Cao, J;Velez, M;Hoeppner, C;Soviero, S;Lawrence, HJ;Analytical performance of a PCR assay for the detection of KRAS mutations (codons 12/13 and 61) in formalin-fixed paraffin-embedded tissue samples of colorectal carcinomaVirchows Arch.22173329Material and methods. Materials. FFPET specimens from CRC tumors were purchased from US commercial vendors: Discovery Life Sciences, Inc. (Los Osos, CA, USA); BioServe (Beltsville, MD, USA); ProteoGenex (Culver City, CA, USA); CureLine, IncFull Paper
2012Hughes, JA;Lynch, FC;Transhepatic removal of an inferior vena cava filterJ Vasc Interv Radiol22720899Full Paper
2012Lynch, FC;Modified loop snare technique for the removal of bard recovery, G2, G2 express, and eclipse inferior vena cava filtersJ Vasc Interv Radiol22525025Full Paper
2012Vijay, K;Hughes, JA;Burdette, AS;Scorza, LB;Singh, H;Waybill, PN;Lynch, FC;Fractured Bard Recovery, G2, and G2 express inferior vena cava filters: incidence, clinical consequences, and outcomes of removal attemptsJ Vasc Interv Radiol22173108Full Paper
2012Mutka, SC;Green, LH;Verderber, EL;Richards, JP;Looker, DL;Chlipala, EA;Rosenthal, GJ;ADH IB expression, but not ADH III, is decreased in human lung cancerPLoS ONE23285246GSNOR immunohistochemistry. Immunohistochemical analysis for GSNOR was performed using formalin-fixed, paraffin-embedded human lung tissue microarrays (Folio Bioscience, Columbus, OH; #ARY-HH0178, #ARY-HH0176)Full Paper
2012Ueki, A;Shimizu, T;Masuda, K;Yamaguchi, SI;Ishikawa, T;Sugihara, E;Onishi, N;Kuninaka, S;Miyoshi, K;Muto, A;Toyama, Y;Banno, K;Aoki, D;Saya, H;Up-regulation of Imp3 confers in vivo tumorigenicity on murine osteosarcoma cellsPLoS ONE23226335Zeiss). Human Osteosarcoma Tissue Array An array of human osteosarcoma specimens was obtained from Folio Biosciences (Powell, OH) and was subjected to immunohis- tochemical staining for IMP3 as described above ; Human Osteosarcoma Tissue Array.Full Paper
2012Xu, J;Ji, P;Zhao, Z;Zhang, Y;Feng, J;Wang, J;Li, J;Zhang, X;Zhao, L;Liu, G;Xu, P;Sun, X;Genome-wide SNP discovery from transcriptome of four common carp strainsPLoS ONE23110192;... e distribution of all samples were checked with Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, USA). cDNA Library Construction Illumina Sequencing Sequencing of 24 samples was conducted in HudsonAlpha Genomic Services Laboratory (HuntFull Paper
2012Hedin, M;Starrett, J;Akhter, S;Schönhofer, AL;Shultz, JW;Phylogenomic resolution of paleozoic divergences in harvestmen (Arachnida, Opiliones) via analysis of next-generation transcriptome dataPLoS ONE22936998Protolophus total RNA was shipped to Cofactor Genomics (www.cofactorgenomics.com), where a duplex-specific nuclease (DSN) normalized library was constructed, and 60 basepair (bp) paired-end reads were generated using Illumina GAII technology. All othFull Paper
2012Johansson, S;Irgens, H;Chudasama, KK;Molnes, J;Aerts, J;Roque, FS;Jonassen, I;Levy, S;Lima, K;Knappskog, PM;Bell, GI;Molven, A;Njølstad, PR;Exome sequencing and genetic testing for MODYPLoS ONE22662265Targeted capture and massive parallel sequencing were performed at HudsonAlpha Institute for Biotechnology (Huntsville, AL) (File S1). In brief, SureSelect Human All Exon Kit (Agilent Technologies, Santa Clara, CA) was used for exome enrichment, andFull Paper
2012Myers, RB;Rwayitare, K;Richey, L;Lem, J;Castellot, JJ;CCN5 Expression in mammals. III. Early embryonic mouse developmentJ Cell Commun Signal22926930earlier, at the 4-cell stage. Materials and methods. Immunohistochemistry. 5 μm frozen sagittal sections of C57BL/6 post-implantation embryos were obtained from Folio Bio (Columbus, OH). Sections were cleared with xyleneFull Paper
2012Ding, D;Scott, NM;Thompson, EE;Chaiworapongsa, T;Torres, R;Billstrand, C;Murray, K;Dexheimer, PJ;Ismail, M;Kay, H;Levy, S;Romero, R;Lindheimer, MD;Nicolae, DL;Ober, C;Increased protein-coding mutations in the mitochondrial genome of African American women with preeclampsiaReprod Sci22902742Mitochondrial genome libraries were prepared by amplification of the complete mtDNA genome using 2 sets of overlapping primers. These fragments were pooled in molar equivalent amounts, sonicated to randomly fragment the DNA, and then prepared for seqFull Paper
2012Morimoto, M;Souich, Cd;Trinh, J;McLarren, KW;Boerkoel, CF;Hendson, G;Expression profile of NSDHL in human peripheral tissuesJ. Mol. Histol.22113624This construct was cut at unique NotI and SpeI sites flanking the Nsdhl insert and transcribed by T3 and T7 RNA polymerases to yield antisense and sense in situ hybridization probes, respectively. In situ hybridization was performed by Phylogeny, IncFull Paper
2012Wang, R;Li, C;Stoeckel, J;Moyer, G;Liu, Z;Peatman, E;Rapid development of molecular resources for a freshwater mussel, Villosa lienosa (Bivalvia:Unionidae), using an RNA-seq-based approachFreshwater ScienceAfter quantitation with KAPA Library Quant Kits (Kapa Biosystems, Woburn, Massachusetts) and dilution, the libraries were clustered 4 per lane and sequenced on a HiSeq 2000 instrument with 100-bp PE reads at the HudsonAlpha Genomic Services LaboratorFull Paper
2012Kishida, M;Ishige, K;Horibe, T;Tada, N;Koibuchi, N;Shoda, J;Kita, K;Kawakami, K;Orexin 2 receptor as a potential target for immunotoxin and antibody-drug conjugate cancer therapyOncol Lett22740944Materials and methods. Materials. Hepatocellular carcinoma tissue array, ARY- HH0075 was purchased from Folio Biosciences (Columbus, OH, USA). Digestive system disease tissue array, DID381 was purchased from US Biomax (Rockville, MD, USA)Full Paper
2012Kentsis, A;Reed, C;Rice, KL;Sanda, T;Rodig, SJ;Tholouli, E;Christie, A;Valk, PJ;Delwel, R;Ngo, V;Kutok, JL;Dahlberg, SE;Moreau, LA;Byers, RJ;Christensen, JG;Vande Woude, G;Licht, JD;Kung, AL;Staudt, LM;Look, AT;Autocrine activation of the MET receptor tyrosine kinase in acute myeloid leukemiaNature medicine22683780This report identifies upregulation of HGF as an autocrine growth pathway in several subsets of AML. Ligand-dependent activation of MET represents a new oncogenic stimulus, and the SaveFull Paper
2012Schenk, T;Chen, WC;Göllner, S;Howell, L;Jin, L;Hebestreit, K;Klein, HU;Popescu, AC;Burnett, A;Mills, K;Casero, RA;Marton, L;Woster, P;Minden, MD;Dugas, M;Wang, JC;Dick, JE;Müller-Tidow, C;Petrie, K;Zelent, A;Inhibition of the LSD1 (KDM1A) demethylase reactivates the all-trans-retinoic acid differentiation pathway in acute myeloid leukemiaNature medicine22406747We grew normal bone marrow mononuclear cells (AllCells, ABM011F) in IMDM supplemented with 20 ng ml −1 SCF, 10 ng ml −1 each of IL-3, IL-6 and fms-related tyrosine kinase 3 ( SaveFull Paper
2012Zhang, C;Srinivasan, Y;Arlow, DH;Fung, JJ;Palmer, D;Zheng, Y;Green, HF;Pandey, A;Dror, RO;Shaw, DE;Weis, WI;Coughlin, SR;Kobilka, BK;High-resolution crystal structure of human protease-activated receptor 1Nature23222541In brief, acid-citrate-dextrose anti-coagulated human blood samples (60 ml per donor) were obtained from AllCells. Blood was centrifuged without braking at 250g at 37 C for 15 min. SaveFull Paper
2012Sasaki, M;Knobbe, CB;Munger, JC;Lind, EF;Brenner, D;Brüstle, A;Harris, IS;Holmes, R;Wakeham, A;Haight, J;You-Ten, A;Li, WY;Schalm, S;Su, SM;Virtanen, C;Reifenberger, G;Ohashi, PS;Barber, DL;Figueroa, ME;Melnick, A;Zúñiga-Pflücker, JC;Mak, TW;IDH1(R132H) mutation increases murine haematopoietic progenitors and alters epigeneticsNature22763442Two normal CD34 + bone marrow control samples were purchased from AllCells. Institutional review board approval was obtained at Weill Cornell Medical Center and this study was SaveFull Paper
2012Kohn, LA;Hao, QL;Sasidharan, R;Parekh, C;Ge, S;Zhu, Y;Mikkola, HK;Crooks, GM;Lymphoid priming in human bone marrow begins before expression of CD10 with upregulation of L-selectinNature immunology22941246esis in disease states. METHODS Bone marrow cell isolation Normal human BM and thymic cells were obtained from healthy donors via the UCLA Pathology Tissue Core, Cincinnati Children’s Hospital, or ALLCELLS according to guidelines approved by UCLA InFull Paper
2012Zhu, X;Wang, Y;Pi, W;Liu, H;Wickrema, A;Tuan, D;NF-Y recruits both transcription activator and repressor to modulate tissue- and developmental stage-specific expression of human γ-globin genePloS one23071749The human cells were obtained commercially from AllCells and processed with protocols approved by Human Assurance Committee of Georgia Health Sciences University (HAC file # SaveFull Paper
2012Hur, EM;Patel, SN;Shimizu, S;Rao, DS;Gnanapragasam, PN;An, DS;Yang, L;Baltimore, D;Inhibitory effect of HIV-specific neutralizing IgA on mucosal transmission of HIV in humanized miceBlood23065154intraperitoneally with 3 × 10 6 human PBMCs (AllCells) that were activated by PHA (5 μg/mL) NSG mice were transplanted intraperitoneally with 3 × 10 6 human PBMCs (AllCells) that SaveFull Paper
2012Lu, M;Wang, X;Li, Y;Tripodi, J;Mosoyan, G;Mascarenhas, J;Kremyanskaya, M;Najfeld, V;Hoffman, R;Combination treatment in vitro with Nutlin, a small-molecule antagonist of MDM2, and pegylated interferon-α 2a specifically targets JAK2V617F-positive polycythemia vera cellsBlood22872685Fresh normal human bone marrow CD34+ cells were purchased from ALLCELLS. The characteristics of the PV patients studied are shown in supplemental Table 1 (available on the Blood Web site; see the Supplemental Materials link at the top of the online aFull Paper
2012Ndhlovu, LC;Lopez-Vergès, S;Barbour, JD;Jones, RB;Jha, AR;Long, BR;Schoeffler, EC;Fujita, T;Nixon, DF;Lanier, LL;Tim-3 marks human natural killer cell maturation and suppresses cell-mediated cytotoxicityBlood22383801Peripheral blood mononuclear cells (PBMCs) of healthy individuals were obtained from the Stanford Blood Bank. Cord blood PMBCs were obtained from AllCells. The University of California, San Francisco and the University of Hawaii institutional reviewFull Paper
2012Wey, S;Luo, B;Tseng, CC;Ni, M;Zhou, H;Fu, Y;Bhojwani, D;Carroll, WL;Lee, AS;Inducible knockout of GRP78/BiP in the hematopoietic system suppresses Pten-null leukemogenesis and AKT oncogenic signalingBlood21937694The normal peripheral blood mononuclear cells were purchased from ALLCELLS (catalog #PB003F). The ethical use of the human tissues for research was approved by the University SaveFull Paper
2012Cheng, L;Hansen, NF;Zhao, L;Du, Y;Zou, C;Donovan, FX;Chou, BK;Zhou, G;Li, S;Dowey, SN;Ye, Z;NISC Comparative Sequencing Program, ;Chandrasekharappa, SC;Yang, H;Mullikin, JC;Liu, PP;Low incidence of DNA sequence variation in human induced pluripotent stem cells generated by nonintegrating plasmid expressionCell stem cell22385660blood of anonymous donors were collected and processed at AllCells, LLC (Emeryville, CA). The by Institutional Review Board (IRB) at AllCells. Use of anonymous human samples for SaveFull Paper
2012Smith, MR;Atkinson, P;White, D;Piersma, T;Gutierrez, G;Rossini, G;Desai, S;Wellinghoff, S;Yu, H;Cheng, X;Design and assessment of a wrapped cylindrical Ca-P AZ31 Mg alloy for critical-size ulna defect repairJournal of biomedical materials research. Part B, Applied biomaterials22102519AZ31 Mg alloy cupons were prepared as described above and sterilized using ethylene oxide gas. Two cell migration tests were performed: indirect and direct. Frozen human MSCs (AllCells, LLC, Emeryville, CA) were thawed, spun down, and resuspended inFull Paper
2012Muthuswamy, R;Berk, E;Junecko, BF;Zeh, HJ;Zureikat, AH;Normolle, D;Luong, TM;Reinhart, TA;Bartlett, DL;Kalinski, P;NF-κB hyperactivation in tumor tissues allows tumor-selective reprogramming of the chemokine microenvironment to enhance the recruitment of cytolytic T effector cellsCancer research22593190vein endothelial cells (HUVEC; AllCells) were cultured in HUVEC complete media (Basal media supplemented with HUVEC stimulatory supplement; AllCells). All cell lines were washed SaveFull Paper
2012Capozza, F;Trimmer, C;Castello-Cros, R;Katiyar, S;Whitaker-Menezes, D;Follenzi, A;Crosariol, M;Llaverias, G;Sotgia, F;Pestell, RG;Lisanti, MP;Genetic ablation of Cav1 differentially affects melanoma tumor growth and metastasis in mice: role of Cav1 in Shh heterotypic signaling and transendothelial migrationCancer research22396494B16F10 and A-375 were from ATCC, while human immortalized dermal foreskin fibroblasts (hTBJ1) were originally from Clontech. Human Umbilical Vein Endothelial Cells (HUVEC) were purchased from ALLCELLS. Only early passages of these cell lines were useFull Paper
2012Silverman, AM;Nakata, R;Shimada, H;Sposto, R;DeClerck, YA;A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastomaCancer research22389450Interleukin-6 (IL-6) is a pleiotropic cytokine with a broad range of physiologic and pathologic functions. Because in cancer, IL-6 contributes to a microenvironment that promotes tumor SaveFull Paper
2012Pacienza, N;Yoshimitsu, M;Mizue, N;Au, BC;Wang, JC;Fan, X;Takenaka, T;Medin, JA;Lentivector transduction improves outcomes over transplantation of human HSCs alone in NOD/SCID/Fabry miceMolecular therapy : the journal of the American Society of Gene Therapy22472949Human mobilized PB CD34+ cells were obtained from AllCells (Emeryville, CA). These cells were rapidly thawed following the manufacturer's recommendations. After thawing, ~85% of the cells were viable as assessed by trypan blue exclusion, while the hCFull Paper
2012Lee, EJ;Choi, EK;Kang, SK;Kim, GH;Park, JY;Kang, HJ;Lee, SW;Kim, KH;Kwon, JS;Lee, KH;Ahn, Y;Lee, HJ;Cho, HJ;Choi, SJ;Oh, WI;Park, YB;Kim, HS;N-cadherin determines individual variations in the therapeutic efficacy of human umbilical cord blood-derived mesenchymal stem cells in a rat model of myocardial infarctionMolecular therapy : the journal of the American Society of Gene Therapy22068423PBMCs (1 × 105/well) or hUCB-MSCs (1 × 102, 1 × 103 or 1 × 104/well) in 96-well tissue culture plates. The PBMCs were purchased from AllCells (Emeryville, CA) The stimulator PBMCs and hUCB-MSCs were inactivated by treatment with 10 µg/ml mitomycin-CFull Paper
2012Goh, KC;Novotny-Diermayr, V;Hart, S;Ong, LC;Loh, YK;Cheong, A;Tan, YC;Hu, C;Jayaraman, R;William, AD;Sun, ET;Dymock, BW;Ong, KH;Ethirajulu, K;Burrows, F;Wood, JM;TG02, a novel oral multi-kinase inhibitor of CDKs, JAK2 and FLT3 with potent anti-leukemic propertiesLeukemia21860433Primary cells—either peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients—were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex ( SaveFull Paper
2012Boitano, AE;de Lichtervelde, L;Snead, JL;Cooke, MP;Schultz, PG;An image-based screen identifies a small molecule regulator of megakaryopoiesisProceedings of the National Academy of Sciences of the United States of America22891346Human CD34+ cells were purified from fresh human leukophoresed-mobilized peripheral blood (AllCells) or from umbilical-cord blood (Bioreclamation), as noted, using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following manufacturer'sFull Paper
2012Kobune, M;Iyama, S;Kikuchi, S;Horiguchi, H;Sato, T;Murase, K;Kawano, Y;Takada, K;Ono, K;Kamihara, Y;Hayashi, T;Miyanishi, K;Sato, Y;Takimoto, R;Kato, J;Stromal cells expressing hedgehog-interacting protein regulate the proliferation of myeloid neoplasmsBlood cancer journal22961059Minneapolis, MN, USA). 5-Aza-2′-deoxycytidine (5-aza-dC) was obtained from Sigma (St Louis, MO, USA). Total RNA derived from mobilized peripheral blood (PB), BM or CB CD34+ cells was purchased from AllCells, LLC (Toronto, Ontario, Canada). Human myelFull Paper
2012Novotny-Diermayr, V;Hart, S;Goh, KC;Cheong, A;Ong, LC;Hentze, H;Pasha, MK;Jayaraman, R;Ethirajulu, K;Wood, JM;The oral HDAC inhibitor pracinostat (SB939) is efficacious and synergistic with the JAK2 inhibitor pacritinib (SB1518) in preclinical models of AMLBlood cancer journal22829971Peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex (Culver City, CA, USA) SaveFull Paper
2012Gao, N;Cheng, S;Budhraja, A;Gao, Z;Chen, J;Liu, EH;Huang, C;Chen, D;Yang, Z;Liu, Q;Li, P;Shi, X;Zhang, Z;Ursolic acid induces apoptosis in human leukaemia cells and exhibits anti-leukaemic activity in nude mice through the PKB pathwayBritish journal of pharmacology21950524Blasts were suspended at 8 × 105 mL-1 and incubated in RPMI 1640 medium containing 10% FBS in 24-well plates. Fresh normal bone marrow mononuclear cells were purchased from Allcells (Emeryvill, CA). After being washed and counted, cells were suspendeFull Paper
2012De Giovanni, C;Nicoletti, G;Landuzzi, L;Romani, F;Croci, S;Palladini, A;Murgo, A;Antognoli, A;Ianzano, ML;Stivani, V;Grosso, V;Iezzi, M;Stramucci, L;Barbieri, E;Lemoli, RM;Nanni, P;Lollini, PL;Human responses against HER-2-positive cancer cells in human immune system-engrafted miceBritish journal of cancer22929887ec, Bergisch Gladbach, Germany) to obtain highly purified cells (mean purity 93%±6%) as previously described (Rossi et al, 2007). For challenge experiments, CD34+ or CD133+ cells were purchased from AllCells (Emeryville, CA, USA). All the experimentsFull Paper
2012Fukushima, H;Kawanabe, N;Murata, S;Ishihara, Y;Yanagita, T;Balam, TA;Yamashiro, T;SSEA-4 is a marker of human deciduous periodontal ligament stem cellsJournal of dental research22895512Human bone marrow cells were purchased from AllCells (Emeryville, CA, USA) and were maintained in low-glucose DMEM supplemented with 10% HIFBS and penicillin/ streptomycin. SaveFull Paper
2012Kim, J;Denu, RA;Dollar, BA;Escalante, LE;Kuether, JP;Callander, NS;Asimakopoulos, F;Hematti, P;Macrophages and mesenchymal stromal cells support survival and proliferation of multiple myeloma cellsBritish journal of haematology22583117Bone marrow (BM) microenvironment provides crucial supports to normal and malignant hematopoiesis and several types of cells such as endothelial cells, osteoblasts and SaveFull Paper
2012Cokic, VP;Bhattacharya, B;Beleslin-Cokic, BB;Noguchi, CT;Puri, RK;Schechter, AN;JAK-STAT and AKT pathway-coupled genes in erythroid progenitor cells through ontogenyJournal of translational medicine22676255mmunomagnetic selection with MACS cell isolation system (Miltenyi Biotec, Auburn, CA). Commercial FL-derived CD34+ cells (Cambrex Bio Science, Inc., Walkersville, MD), CB- and BM-derived CD34+ cells (AllCells LLC, Berkeley, CA) are also isolated by pFull Paper
2012Mao, M;Sudhahar, V;Ansenberger-Fricano, K;Fernandes, DC;Tanaka, LY;Fukai, T;Laurindo, FR;Mason, RP;Vasquez-Vivar, J;Minshall, RD;Stadler, K;Bonini, MG;Nitroglycerin drives endothelial nitric oxide synthase activation via the phosphatidylinositol 3-kinase/protein kinase B pathwayFree radical biology & medicine22037515Human and bovine aortic endothelial cells (HAEC or BAEC; Allcells, Emeryville, CA, USA), human microvascular endothelial cells (HMEC), and primary mouse endothelial cells (MEC) were cultured at 37 °C, 5% CO2 in corresponding endothelial medium with sFull Paper
2012William, AD;Lee, AC;Poulsen, A;Goh, KC;Madan, B;Hart, S;Tan, E;Wang, H;Nagaraj, H;Chen, D;Lee, CP;Sun, ET;Jayaraman, R;Pasha, MK;Ethirajulu, K;Wood, JM;Dymock, BW;Discovery of the macrocycle (9E)-15-(2-(pyrrolidin-1-yl)ethoxy)-7,12,25-trioxa-19,21,24-triaza-tetracyclo[18.3.1.1(2,5).1(14,18)]hexacosa-1(24),2,4,9,14(26),15,17,20,22-nonaene (SB1578), a potent inhibitor of janus kinase 2/fms-like tyrosine kinase-3 (JAK2/FLT3) for the treatment of rheumatoid arthritisJournal of medicinal chemistry22339472These cells were cultured as described previously. (34) Human CD4+ T cells, isolated from peripheral blood mononuclear cells with 95% purity, were purchased from AllCells (Emeryville, CA) and maintained in X-VIVO 20 media (Lonza, Basel, Switzerland)Full Paper
2012Tran, CA;Torres-Coronado, M;Gardner, A;Gu, A;Vu, H;Rao, A;Cao, LF;Ahmed, A;Digiusto, D;Optimized processing of growth factor mobilized peripheral blood CD34+ products by counterflow centrifugal elutriationStem cells translational medicine23197821HPC-A products were obtained from AllCells LLC (Emeryville, CA, http://www.allcells.com), Key Biologics LLC (Memphis, TN, http://www.keybiologics.com), or Progenitor Cell Therapy LLC (Mountain View, CA, http://www.pctcelltherapy.com). HPC-A productsFull Paper
2012Rodriguez-Menocal, L;Salgado, M;Ford, D;Van Badiavas, E;Stimulation of skin and wound fibroblast migration by mesenchymal stem cells derived from normal donors and chronic wound patientsStem cells translational medicine23197781We isolated and expanded human MSCs from fresh whole bone marrow obtained from normal donors and chronic wound patients. Normal donor bone marrow was acquired from AllCells LLC (Emeryville, CA, http://www.allcells.com). Chronic wound patient bone marFull Paper
2012Oskouei, BN;Lamirault, G;Joseph, C;Treuer, AV;Landa, S;Da Silva, J;Hatzistergos, K;Dauer, M;Balkan, W;McNiece, I;Hare, JM;Increased potency of cardiac stem cells compared with bone marrow mesenchymal stem cells in cardiac repairStem cells translational medicine23197758Human bone marrow aspirates were obtained from AllCells LLC (Emeryville, CA, http://allcells.com). Low-density mononuclear cells were separated from human bone marrow aspirates and cultured as described (see supplemental online data). Adherent MSCs wFull Paper
2012Soler, R;Füllhase, C;Hanson, A;Campeau, L;Santos, C;Andersson, KE;Stem cell therapy ameliorates bladder dysfunction in an animal model of Parkinson diseaseThe Journal of urology22341818Different cell based therapies have been tested, focusing on motor function. We evaluated the effect of human amniotic fluid stem cells and bone marrow derived mesenchymal stem cells (ALLCELLS, Emeryville, California) on bladder dysfunction in a ratFull Paper
2012Hirata, Y;Ikeda, K;Sudoh, M;Tokunaga, Y;Suzuki, A;Weng, L;Ohta, M;Tobita, Y;Okano, K;Ozeki, K;Kawasaki, K;Tsukuda, T;Katsume, A;Aoki, Y;Umehara, T;Sekiguchi, S;Toyoda, T;Shimotohno, K;Soga, T;Nishijima, M;Taguchi, R;Kohara, M;Self-enhancement of hepatitis C virus replication by promotion of specific sphingolipid biosynthesisPLoS pathogens22916015les containing 200 µg protein were assayed for SPT activity using [14C]-serine and palmitoyl-CoA (Sigma-Aldrich, St. Louis, MO, USA) as substrates. Proliferation assay Human peripheral blood cells (AllCells, Emeryville, CA, USA) were plated onto 96-Full Paper
2012Köllmer, M;Keskar, V;Hauk, TG;Collins, JM;Russell, B;Gemeinhart, RA;Stem cell-derived extracellular matrix enables survival and multilineage differentiation within superporous hydrogelsBiomacromolecules22404228Human bone marrow aspirates were obtained from AllCells, LLC (Emeryville, CA) and isolated by density gradient centrifugation utilizing Ficoll-Paque PLUS solution and RosetteSep SaveFull Paper
2012Sharma, SM;Fuchsluger, T;Ahmad, S;Katikireddy, KR;Armant, M;Dana, R;Jurkunas, UV;Comparative analysis of human-derived feeder layers with 3T3 fibroblasts for the ex vivo expansion of human limbal and oral epitheliumStem cell reviews and reports21964568Bone marrow samples (3-10 ml) were purchased from AllCells (Emeryville, CA) and plated in T175 flasks in growth media consisting of α-MEM (Lonza, Walkersville, MD) supplemented SaveFull Paper
2012McNamara, LA;Ganesh, JA;Collins, KL;Latent HIV-1 infection occurs in multiple subsets of hematopoietic progenitor cells and is reversed by NF-κB activationJournal of virology22718820bone marrow (BM) was obtained commercially (AllCells Ltd.); mononuclear cells were purified Presorted CD133 + BM or CB cells were obtained commercially (AllCells Ltd.) and were SaveFull Paper
2012Yu, X;McCarthy, PJ;Wang, Z;Gorlen, DA;Mertz, JE;Shutoff of BZLF1 gene expression is necessary for immortalization of primary B cells by Epstein-Barr virusJournal of virology22623769B-cell proliferation assay. Human primary blood B cells were purchased from AllCells (Emeryville, CA), infected with the indicated amount of virus in green Raji units (GRU)/cell, aliquoted at 1 × 104 cells per well in 96-well plates, incubated at 37°Full Paper
2012Nan, XW;Tang, GJ;Xu, JG;Peng, TS;Miao, LS;Xu, ZG;Bai, C;Yu, P;Gao, YL;Hao, BT;Imaging manifestations of renal primary neuroectodermal tumorsChinese medical journal23044335Pathologicaland jmm unohistochemicalfindingsshowed portionofthetum ornecrosedanduni—morp hologicaloval orround sm allcells with rosettes structure underlight mi croscope.Im SaveFull Paper
2012Lin, W;Oh, SK;Choo, AB;George, AJ;Activated T cells modulate immunosuppression by embryonic-and bone marrow-derived mesenchymal stromal cells through a feedback mechanismCytotherapy22136295Frozen peripheral blood mononuclear cells (PBMC) were purchased from Allcells (Emeryville, CA, USA). All isolated T-cell cultures were maintained in Roswell Park Memorial Institute (RPMI)-1640 supplemented with 10% FBS, 2 mm l-glutamine, 50 U/mL peniFull Paper
2012Akalin, A;Garrett-Bakelman, FE;Kormaksson, M;Busuttil, J;Zhang, L;Khrebtukova, I;Milne, TA;Huang, Y;Biswas, D;Hess, JL;Allis, CD;Roeder, RG;Valk, PJ;Löwenberg, B;Delwel, R;Fernandez, HF;Paietta, E;Tallman, MS;Schroth, GP;Mason, CE;Melnick, A;Figueroa, ME;Base-pair resolution DNA methylation sequencing reveals profoundly divergent epigenetic landscapes in acute myeloid leukemiaPLoS genetics22737091kemia gene rearranged AML samples harboring t(9;11) (MLL1 and MLL2) and one additional AML sample (AML) were available for processing. Two normal CD34+ bone marrow control samples were purchased from AllCells, LLC (Emeryville, CA, USA). InstitutionalFull Paper
2012Chaudhry, P;Yang, X;Wagner, M;Jong, A;Wu, L;Retinoid-regulated FGF8f secretion by osteoblasts bypasses retinoid stimuli to mediate granulocytic differentiation of myeloid leukemia cellsMolecular cancer therapeutics22135230Normal human primitive hematopoietic CD34 + cells were obtained from AllCells and CD34 cells, certified to be HIV- and Mycoplasma-free by AllCells, were cultured for maxi SaveFull Paper
2012Budhraja, A;Gao, N;Zhang, Z;Son, YO;Cheng, S;Wang, X;Ding, S;Hitron, A;Chen, G;Luo, J;Shi, X;Apigenin induces apoptosis in human leukemia cells and exhibits anti-leukemic activity in vivoMolecular cancer therapeutics22084167U937, Jurkat, and HL60 human leukemia cells were obtained from American Type Culture Collection and grown in RPMI-1640 medium with 10% FBS. Normal peripheral blood mononuclear cells (NPBMNC) were obtained from AllCells and maintained in RPMI-1640 witFull Paper
2012de Lichtervelde, L;Antal, CE;Boitano, AE;Wang, Y;Krastel, P;Petersen, F;Newton, AC;Cooke, MP;Schultz, PG;Euphohelioscopin A is a PKC activator capable of inducing macrophage differentiationChemistry & biology22921066R & D Systems]) unless otherwise indicated. Human mPB CD34+ cells were purified from fresh human leukophoresed mobilized peripheral blood (AllCells) using direct CD34 progenitor cell isolation kits (Miltenyi Biotec) following manufacturer’s protocolsFull Paper
2012Qu, X;Zhuang, G;Yu, L;Meng, G;Ferrara, N;Induction of Bv8 expression by granulocyte colony-stimulating factor in CD11b+Gr1+ cells: key role of Stat3 signalingThe Journal of biological chemistry22528488Freshly isolated bone marrow cells from five different healthy donors were obtained from AllCells (Emeryville, CA). Red blood cells were lysed by adding 4 volumes of 1× buffered SaveFull Paper
2012Rowe, DD;Leonardo, CC;Recio, JA;Collier, LA;Willing, AE;Pennypacker, KR;Human umbilical cord blood cells protect oligodendrocytes from brain ischemia through Akt signal transductionThe Journal of biological chemistry22158864Human umbilical cord blood (HUCB) cells protect the brain against ischemic injury, yet the mechanism of protection remains unclear. Using both in vitro and in vivo paradigms, this SaveFull Paper
2012Madan, B;Goh, KC;Hart, S;William, AD;Jayaraman, R;Ethirajulu, K;Dymock, BW;Wood, JM;SB1578, a novel inhibitor of JAK2, FLT3, and c-Fms for the treatment of rheumatoid arthritisJournal of immunology (Baltimore, Md. : 1950)22962687Cells CD4+ T cells isolated from human PBMCs, with 95% purity were obtained from AllCells (Emeryville, CA). The THP-1 cell line was purchased from American Type Culture Collection and maintained as recommendedFull Paper
2012Liu, W;Yan, M;Liu, Y;McLeish, KR;Coleman, WG;Rodgers, GP;Olfactomedin 4 inhibits cathepsin C-mediated protease activities, thereby modulating neutrophil killing of Staphylococcus aureus and Escherichia coli in miceJournal of immunology (Baltimore, Md. : 1950)22844115Human fresh bone-marrow neutrophils were obtained from AllCells (Emeryville, CA). Mouse neutrophils were isolated from bone marrow. The femur was removed from mice, then SaveFull Paper
2012Ohtsubo, S;Ishikawa, M;Kamei, N;Kijima, Y;Suzuki, O;Sunagawa, T;Higashi, Y;Masuda, H;Asahara, T;Ochi, M;The therapeutic potential of ex vivo expanded CD133+ cells derived from human peripheral blood for peripheral nerve injuriesJournal of neurosurgery22880720We used granulocyte-colony stimulating factor-mobilized CD133+ cells derived from human peripheral blood (lot no. PCA1521B, AllCells) for these experiments. The fresh CD133+ cells were cultured with serum-free medium (StemSpan, Stem Cell TechnologiesFull Paper
2012Kadam, SK;Farmen, M;Brandt, JT;Quantitative measurement of cell-free plasma DNA and applications for detecting tumor genetic variation and promoter methylation in a clinical settingThe Journal of molecular diagnostics : JMD22579630Commercially available pooled human plasma (AllCells, Emeryville, CA) was used to determine matrix effect and for DNA spike-in experiments. All blood samples from healthy controls SaveFull Paper
2012Magga, J;Savchenko, E;Malm, T;Rolova, T;Pollari, E;Valonen, P;Lehtonen, Š;Jantunen, E;Aarnio, J;Lehenkari, P;Koistinaho, M;Muona, A;Koistinaho, J;Production of monocytic cells from bone marrow stem cells: therapeutic usage in Alzheimer's diseaseJournal of cellular and molecular medicine21777378Flt-3, 20 ng/ml IL-6, 20 ng/ml IL-3, in humidified atmosphere at 37°C in 5% CO2. Cells were cultivated and differentiated as described earlier. Human PB GCSF-mobilized CD34 cells were obtained from AllCells and cultivated similarly to BM-originated cFull Paper
2012Xia, Y;Wübbeler, JH;Qi, Q;Steinbüchel, A;Employing a recombinant strain of Advenella mimigardefordensis for biotechnical production of Homopolythioesters from 3,3'-dithiodipropionic acidApplied and environmental microbiology22344658AftertheirODhadreached500Klettunits,allcells were transferred into the same MS medium containing 7 g/liter glycerol plus 9 g/liter DTDP as a precursor. (A) Increase of OD (Klett unit) SaveFull Paper
2012Pei, L;Choi, JH;Liu, J;Lee, EJ;McCarthy, B;Wilson, JM;Speir, E;Awan, F;Tae, H;Arthur, G;Schnabel, JL;Taylor, KH;Wang, X;Xu, D;Ding, HF;Munn, DH;Caldwell, C;Shi, H;Genome-wide DNA methylation analysis reveals novel epigenetic changes in chronic lymphocytic leukemiaEpigenetics22534504CD19+/CD27+ memory B-cell, and CD19+/CD27- naïve B-cell samples used in the RRBS analysis were purchased from ALLCELLs Inc. Other normal CD19+ B-cell samples were purified from leuko-enriched blood samples purchased from a local blood bank. MononucleFull Paper
2012Behbehani, GK;Bendall, SC;Clutter, MR;Fantl, WJ;Nolan, GP;Single-cell mass cytometry adapted to measurements of the cell cycleCytometry. Part A : the journal of the International Society for Analytical Cytology22693166Mass cytometry is a recently introduced technology that utilizes transition element isotope‐tagged antibodies for protein detection on a single‐cell basis. By circumventing the limitations SaveFull Paper
2012Kanagal-Shamanna, R;Zhao, W;Vadhan-Raj, S;Nguyen, MH;Fernandez, MH;Medeiros, LJ;Bueso-Ramos, CE;Over-expression of CYP2E1 mRNA and protein: implications of xenobiotic induced damage in patients with de novo acute myeloid leukemia with inv(16)(p13.1q22); CBFβ-MYH11International journal of environmental research and public health23066397Island, NY, USA). cDNA synthesis was performed using the QuantiTect RT Kit (Qiagen, Valencia, CA, USA) according to manufacturer’s instructions [14]. The commercially produced normal BM CD34 cDNA (AllCells, LLC, Emeryville, CA, USA) was also used inFull Paper
2012Puri, N;Sodhi, K;Haarstad, M;Kim, DH;Bohinc, S;Foglio, E;Favero, G;Abraham, NG;Heme induced oxidative stress attenuates sirtuin1 and enhances adipogenesis in mesenchymal stem cells and mouse pre-adipocytesJournal of cellular biochemistry22234917For human MSCs, frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential SaveFull Paper
2012Spencer, R;Magnetic resonance in tissue engineeringNMR in biomedicine22134896Mesenchymal stem cells isolated from fresh adult human bone marrow were obtained commercially by AllCells (AllCells LLC, Berkeley, CA, USA). Nucleated cells were incubated in a SaveFull Paper
2012Guo, LW;Wu, Q;Green, B;Nolen, G;Shi, L;Losurdo, J;Deng, H;Bauer, S;Fang, JL;Ning, B;Cytotoxicity and inhibitory effects of low-concentration triclosan on adipogenic differentiation of human mesenchymal stem cellsToxicology and applied pharmacology22726953Human adult mesenchymal stem cells (hMSCs) were originally provided by AllCells (Emeryville, CA). hMSCs were grown in alpha Minimum Essential Medium (αMEM), supplemented SaveFull Paper
2012Xu, D;Kim, Y;Postelnek, J;Vu, MD;Hu, DQ;Liao, C;Bradshaw, M;Hsu, J;Zhang, J;Pashine, A;Srinivasan, D;Woods, J;Levin, A;O'Mahony, A;Owens, TD;Lou, Y;Hill, RJ;Narula, S;DeMartino, J;Fine, JS;RN486, a selective Bruton's tyrosine kinase inhibitor, abrogates immune hypersensitivity responses and arthritis in rodentsThe Journal of pharmacology and experimental therapeutics22228807One million human cord blood-derived CD34+ hematopoietic stem cells (AllCells, LLC, Emeryville, CA) were cultured in a serum-free StemPro-34 complete medium (Invitrogen) with supplements, stem cell factor (100 ng/ml), and IL-6 (50 ng/ml). IL-3 (10 ngFull Paper
2012Pirlo, RK;Wu, P;Liu, J;Ringeisen, B;PLGA/hydrogel biopapers as a stackable substrate for printing HUVEC networks via BioLPBiotechnology and bioengineering21830203Two major challenges in tissue engineering are mimicking the native cell-cell arrangements of tissues and maintaining viability of three‐dimension (3D) tissues thicker than 300 µm. SaveFull Paper
2012Zou, C;Chou, BK;Dowey, SN;Tsang, K;Huang, X;Liu, CF;Smith, C;Yen, J;Mali, P;Zhang, YA;Cheng, L;Ye, Z;Efficient derivation and genetic modifications of human pluripotent stem cells on engineered human feeder cell linesStem cells and development22225458Bone marrow-derived MSCs were established from CD34-depleted mononuclear cells (MNCs) (AllCells) as previously described [16], from adult donors (such as a male donor #BM2426). Primary MSCs and immortalized MSCs were cultured in Dulbecco's modified EFull Paper
2012Aslanidi, GV;Rivers, AE;Ortiz, L;Govindasamy, L;Ling, C;Jayandharan, GR;Zolotukhin, S;Agbandje-McKenna, M;Srivastava, A;High-efficiency transduction of human monocyte-derived dendritic cells by capsid-modified recombinant AAV2 vectorsVaccine22497875DMEM (Invitrogen) supplemented with 10% FBS (Sigma) and antibiotics (Lonza). Leukapheresis-derived peripheral blood mononuclear cells (PBMCs) (AllCells) were purified on Ficoll-Paque (GEHeathCare), resuspended in serum-free AIM-V medium (Lonza), andFull Paper
2012Koestler, DC;Marsit, CJ;Christensen, BC;Accomando, W;Langevin, SM;Houseman, EA;Nelson, HH;Karagas, MR;Wiencke, JK;Kelsey, KT;Peripheral blood immune cell methylation profiles are associated with nonhematopoietic cancersCancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology22714737Sorted, normal, human, peripheral blood leukocyte subtypes were purchased from AllCells. Leukocytes were isolated from different, anonymous, nondiseased individuals' whole blood ( SaveFull Paper
2012Hong, L;Wei, N;Joshi, V;Yu, Y;Kim, N;Krishnamachari, Y;Zhang, Q;Salem, AK;Effects of glucocorticoid receptor small interfering RNA delivered using poly lactic-co-glycolic acid microparticles on proliferation and differentiation capabilities of human mesenchymal stromal cellsTissue engineering. Part A21988716All other chemicals and solvents used, including dichloromethane (DCM) and acetonitrile, were of high-performance liquid chromatography analytical grade. Fresh human bone marrow was purchased from Allcells (Emeryville, CA). Invitrogen (Carlsbad, CA)Full Paper
2012Noto, PB;Bukhtiyarov, Y;Shi, M;McKeever, BM;McGeehan, GM;Lala, DS;Regulation of sphingomyelin phosphodiesterase acid-like 3A gene (SMPDL3A) by liver X receptorsMolecular pharmacology22810003Whole blood from human donors was purchased from AllCells, LLC (Emeryville, CA). Human peripheral blood mononuclear cells (PBMCs) were isolated from the whole blood collected SaveFull Paper
2012Abdolhosseini, M;Nandula, SR;Song, J;Hirt, H;Gorr, SU;Lysine substitutions convert a bacterial-agglutinating peptide into a bactericidal peptide that retains anti-lipopolysaccharide activity and low hemolytic activityPeptides22484285GL13NH2 is a bacteria-agglutinating peptide derived from the sequence of the salivary protein parotid secretory protein (PSP, BPIFA2, SPLUNC2, C20orf70). The peptide agglutinates SaveFull Paper
2012Berezhnyy, IV;Berezhna, SY;Fast multi-spectral imaging technique for detection of circulating endothelial cells in human blood samplesJournal of biomedical optics23224165Blood samples from healthy donors were purchased from AllCells, LLC., Emeryville, CA. The blood was collected via venipuncture in EDTA tubes, stored and delivered at 4°C. Before processing, a white blood cell count was performed using Scepter™ 2.0 ceFull Paper
2012Shah, SN;Kerr, C;Cope, L;Zambidis, E;Liu, C;Hillion, J;Belton, A;Huso, DL;Resar, LM;HMGA1 reprograms somatic cells into pluripotent stem cells by inducing stem cell transcriptional networksPloS one23166588tocol# MO11M279). All mice were housed in a sterile environment where they had free access to food and water as outlined in our institutional guidelines. Cell Culture MSC1640 mesenchymal stem cells (AllCells, LLC) were maintained in DMEM (low glucosFull Paper
2012Ringpis, GE;Shimizu, S;Arokium, H;Camba-Colón, J;Carroll, MV;Cortado, R;Xie, Y;Kim, PY;Sahakyan, A;Lowe, EL;Narukawa, M;Kandarian, FN;Burke, BP;Symonds, GP;An, DS;Chen, IS;Kamata, M;Engineering HIV-1-resistant T-cells from short-hairpin RNA-expressing hematopoietic stem/progenitor cells in humanized BLT micePloS one23300932for 2 hours in the presence of 8 μg/ml of polybrene. Transductions of PBMCs were performed as previously described [23] with indicated cell numbers and MOIs. mPB-CD34+ cells (9.8×104), provided by AllCells (Emeryville, CA), were pre-stimulated with SFull Paper
2012Zhu, J;Siclari, VA;Liu, F;Spatz, JM;Chandra, A;Divieti Pajevic, P;Qin, L;Amphiregulin-EGFR signaling mediates the migration of bone marrow mesenchymal progenitors toward PTH-stimulated osteoblasts and osteocytesPloS one23300521Human bone marrow aspirates were purchased from Allcells and purified through Ficoll gradient (GE Healthcare) to obtain bone marrow mononuclear cells. These cells were seeded SaveFull Paper
2012Wen, H;Li, Y;Malek, SN;Kim, YC;Xu, J;Chen, P;Xiao, F;Huang, X;Zhou, X;Xuan, Z;Mankala, S;Hou, G;Rowley, JD;Zhang, MQ;Wang, SM;New fusion transcripts identified in normal karyotype acute myeloid leukemiaPloS one23251452ry samples with abnormal karyotype AML and eight AML primary samples without karyotype information [Figure 1], of which 38 were from University of Michigan Comprehensive Cancer Center and 7 were from Allcells Inc (AllCells, CA). The use of samples frFull Paper
2012Kobayashi, DT;Decker, D;Zaworski, P;Klott, K;McGonigal, J;Ghazal, N;Sly, L;Chung, B;Vanderlugt, J;Chen, KS;Evaluation of peripheral blood mononuclear cell processing and analysis for Survival Motor Neuron proteinPloS one23226377A: SMN signal was evaluated in lysates created from a single sample purchased from AllCells. PBMCs were processed in ER4 at densities of 10 6 , 10 7 and 5×10 7 cells/mL and the SaveFull Paper
2012Zhang, LJ;Tao, BB;Wang, MJ;Jin, HM;Zhu, YC;PI3K p110α isoform-dependent Rho GTPase Rac1 activation mediates H2S-promoted endothelial cell migration via actin cytoskeleton reorganizationPloS one22970259uman recombinant Rac1 and G-LISA Rac1 activation assay kits were obtained from Cytoskeleton (Denver, CO, USA). Cell culture-Primary human umbilical vein endothelial cells (HUVECs) were obtained from AllCells (Emeryville, CA, USA). Each batch of HUVEFull Paper
2012Venet, S;Ravn, U;Buatois, V;Gueneau, F;Calloud, S;Kosco-Vilbois, M;Fischer, N;Transferring the characteristics of naturally occurring and biased antibody repertoires to human antibody libraries by trapping CDRH3 sequencesPloS one22937053Human CDRH3 from three donors were amplified by nested PCR from commercially available peripheral blood purified cells cDNA (AllCells and BioChain). The first PCR allowed the SaveFull Paper
2012Park, TS;Huo, JS;Peters, A;Talbot, CC;Verma, K;Zimmerlin, L;Kaplan, IM;Zambidis, ET;Growth factor-activated stem cell circuits and stromal signals cooperatively accelerate non-integrated iPSC reprogramming of human myeloid progenitorsPloS one22905176For these experiments, highly purified (>96%) CD34 + CD45 + CB cells were received within 24 hours of neonatal harvest from AllCells (catalog number: CB005). On the same day (day SaveFull Paper
2012Milanesi, A;Lee, JW;Li, Z;Da Sacco, S;Villani, V;Cervantes, V;Perin, L;Yu, JS;β-Cell regeneration mediated by human bone marrow mesenchymal stem cellsPloS one22879915BMSCs from a normal adult donor were purchased from a commercial source AllCells, LLC (5858 Horton Street, Suite 360, Emeryville, California, 94608 USA; catalog number ABM005) SaveFull Paper
2012Boomsma, RA;Geenen, DL;Mesenchymal stem cells secrete multiple cytokines that promote angiogenesis and have contrasting effects on chemotaxis and apoptosisPloS one22558198TCTCCTC GTCTGCCTCTTTTGGTCAGG Angiogenesis Assay The effect of paracrine factors secreted by MSC into CM on angiogenesis was studied using canine jugular vein vascular endothelial cells (CVEC; AllCells) and the Fibrin Gel In Vitro AngiogenesisFull Paper
2012Basile, LA;Ellefson, D;Gluzman-Poltorak, Z;Junes-Gill, K;Mar, V;Mendonca, S;Miller, JD;Tom, J;Trinh, A;Gallaher, TK;HemaMax™, a recombinant human interleukin-12, is a potent mitigator of acute radiation injury in mice and non-human primatesPloS one22383962clear cells (PBMC) in vitro and the PK and PD characteristics of HemaMax in vivo. Isolation of CD14- PBMC and Quantification of IFN-γ Secretion Human PBMC collected by apheresis were purchased from AllCells (Emeryville, CA, USA). Mouse and rhesus moFull Paper
2012Gao, N;Cheng, S;Budhraja, A;Liu, EH;Chen, J;Chen, D;Yang, Z;Luo, J;Shi, X;Zhang, Z;3,3'-Diindolylmethane exhibits antileukemic activity in vitro and in vivo through a Akt-dependent processPloS one22363731lls were washed and assayed for total number and viability using trypan blue exclusion. Cells were suspended at 8×105/mL for treatment. Fresh normal bone marrow mononuclear cells were purchased from Allcells (Emeryvill, CA). After washing and enumeraFull Paper
2012Rellick, SL;O'Leary, H;Piktel, D;Walton, C;Fortney, JE;Akers, SM;Martin, KH;Denvir, J;Boskovic, G;Primerano, DA;Vos, J;Bailey, N;Gencheva, M;Gibson, LF;Bone marrow osteoblast damage by chemotherapeutic agentsPloS one22363485Mediatech), 0.05 µM 2-mercaptoethanol (Sigma-Aldrich, St. Louis, MO), non-essential amino acids and B-FGF solution. H9 cells were moved to HOB feeder layers where indicated. CD34+ bone marrow cells (ALLCELLS, Emeryville, CA) were grown in RPMI-1640 sFull Paper
2012Houseman, EA;Accomando, WP;Koestler, DC;Christensen, BC;Marsit, CJ;Nelson, HH;Wiencke, JK;Kelsey, KT;DNA methylation arrays as surrogate measures of cell mixture distributionBMC bioinformatics22568884ples, de-identified specimens that were not subject to human subjects review by an institutional review board (IRB). The sorted, normal, human, peripheral blood leukocyte subtypes were purchased from AllCellsⓇ, LLC (Emeryville, CA) and were isolatedFull Paper
2012Christopherson, KW;Frank, RR;Jagan, S;Paganessi, LA;Gregory, SA;Fung, HC;CD26 protease inhibition improves functional response of unfractionated cord blood, bone marrow, and mobilized peripheral blood cells to CXCL12/SDF-1Experimental hematology22846168Medical Center (RUMC) with institutional review board (IRB) approval. Human G-CSF mobilized peripheral blood (mobPB) was purchased from AllCells, LLC (Emeryville, CA, USA). Mononuclear cells (MNC) were isolated by density gradient centrifugation overFull Paper
2012Iancu-Rubin, C;Gajzer, D;Mosoyan, G;Feller, F;Mascarenhas, J;Hoffman, R;Panobinostat (LBH589)-induced acetylation of tubulin impairs megakaryocyte maturation and platelet formationExperimental hematology22381681Human MK were generated in liquid cultures from bone marrow (BM)-derived CD34+ cells (AllCells, Inc. Emeryville, CA). Cells were suspended in IMDM medium (Invitrogen, Grand Island, NY) supplemented with 1% penicillin/streptomycin, 1% L-Glutamine (InvFull Paper
2012Fujioka, Y;Tanaka, N;Nakanishi, K;Kamei, N;Nakamae, T;Izumi, B;Ohta, R;Ochi, M;Magnetic field-based delivery of human CD133⁺ cells promotes functional recovery after rat spinal cord injurySpine22246536Phosphate-buffered saline (PBS) solution (50 μL) containing 1 × 10 5 of human peripheral blood-derived CD133 + cells (AllCells, Emeryville, CA) was injected into the subarachnoid SaveFull Paper
2012Huovinen, P;Petersen, H;Particlization in hybrid modelsThe European Physical Journal AThe first one that we call "allcells" sampling, contains a loop over all hypersurface elements The straightforward "allcells" sampling does not do it, and therefore we have developed the SaveFull Paper
2012Kusao, I;Shiramizu, B;Liang, CY;Grove, J;Agsalda, M;Troelstrup, D;Velasco, VN;Marshall, A;Whitenack, N;Shikuma, C;Valcour, V;Cognitive performance related to HIV-1-infected monocytesThe Journal of neuropsychiatry and clinical neurosciences22450616assay for IL6, IL8, IL10, TNFα, sFAS, sTNFRI, sTNFRII, and MCP1. For the in-vitro experiments, monocytes were separated from commercially-available PBMC (AllCells; Emeryville, CA) using the EasySep Human CD14 Positive Selection Kit (Stem Cell TechnolFull Paper
2012Yu, Y;Wei, N;Stanford, C;Schmidt, T;Hong, L;In vitro effects of RU486 on proliferation and differentiation capabilities of human bone marrow mesenchymal stromal cellsSteroids22093480Fresh human bone marrows, donated by healthy donors from 21 to 47 years of age, were purchased from Allcells (Emeryville, CA). Mononuclear cells were isolated using density SaveFull Paper
2012Tatsumi, K;Taatjes, DJ;Wadsworth, MP;Bouchard, BA;Bovill, EG;Cell adhesion molecule 1 (CADM1) is ubiquitously present in the endothelium and smooth muscle cells of the human macro- and micro-vasculatureHistochemistry and cell biology22940845Immunostained cells were visualized with an Olympus BX51 fluorescence microscope (Olympus America, Center Valley, PA). For each experiment (n = 3) human umbilical vein ECs (AllCells, Emeryville CA), cultured as per the manufacturer’s directions, wereFull Paper
2012He, M;Chen, P;Arnovitz, S;Li, Y;Huang, H;Neilly, MB;Wei, M;Rowley, JD;Chen, J;Li, Z;Two isoforms of HOXA9 function differently but work synergistically in human MLL-rearranged leukemiaBlood cells, molecules & diseases22633751The mononuclear cell (MNC) samples were isolated from normal BM cells purchased from AllCells, LLC. MNC cells were isolated using NycoPrep 1.077A (Axis-Shield, Oslo, Norway) Save;in Dulbecco modified Eagle's medium (DMEM) supplemented with 10% FBSFull Paper
2012Kobayashi, M;Kawase, T;Horimizu, M;Okuda, K;Wolff, LF;Yoshie, H;A proposed protocol for the standardized preparation of PRF membranes for clinical useBiologicals : journal of the International Association of Biological Standardization22841724Human umbilical vein endothelial cells (HUVECs) were obtained from AllCells, LLC (Emeryville, CA) and maintained in an endothelial cell medium that was fully supplemented with growth factors (HuMedia-EB2; KURABO, Osaka, Japan). For the scratch assay,Full Paper
2012Aoki, T;Miyazaki, K;Katayama, T;Watanabe, M;Horie, R;Danbara, M;Higashihara, M;Surface CD3 expression proceeds through both myosin regulatory light chain 9 (MYL9)-dependent and MYL9-independent pathways in Jurkat cellsJournal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi23538510K562 cells were transfected using the 96-well Nucleofector Kit SF (Lonza) per manufacturer's recommendations. PBMCs (AllCells) were received on ice, centrifuged at 1,000g for 5 min at room temperature (22 °C) and changed to pre-warmed RPMI medium supFull Paper
2012Wakao, S;Kitada, M;Kuroda, Y;Dezawa, M;Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damagesMethods in molecular biology (Clifton, N.J.)22167642candidate cell source for isolation of Muse cells. We recommend to use adult human dermal fibroblasts (CC-2511, Lonza, Basel, Switzerland; 2320, ScienCell, Carlsbad, CA) and adult human bone marrow stromal cells (PT-2501, Lonza; ABM001, AllCells, EmeFull Paper
2012Mendelson, A;Chondrogenesis of Stem/Progenitor Cells by Chemotaxis Using Novel Cell Homing SystemsThesisHuman bone marrow MSCs were isolated from fresh adult donor samples (AllCells, Emeryville, CA) using our prior methods [94, 96, 115]. Briefly, the bone marrow sample was SaveFull Paper
2012Balzano, G;Liesch, B;The role of chroma and scalestep in the recognition of musical intervals in and out of context.Psychomusicology: A Journal of Research in Music CognitionThe octave presented somewhat of a problem in these analyses; latencies (and errors) tended to be drastically lower in allcells ofthe matrix involving the octave, and because of the SaveFull Paper
2012Gratien, J;Implementing Lowest-Order Methods for Diffusive Problems with a DSELModelling and Simulation in Fluid Dynamics in Porous MediaThe integration algorithm consists in iterating on the cell elements of the allCells(Th) and evaluating the bilinear expression on each cell. This bilinear expression is composed of: SaveFull Paper
2012YAN, LI;Improvements on Humanized NOD/SCID lL-2rg-/-MiceThesisPurified human CD133+ cord blood cells were purchased from AllCells (California). Alternatively, umbilical cord blood was obtained from the Singapore Cord Blood Bank. Red blood SaveFull Paper
2012Nikdel, A;Jameii, SM;Faez, K;A novel area coverage protocol based on particle swarm optimization algorithm for wireless sensor networksGlobal Journal on Technologywill be provided if the sum of cells which are covered (ie Sumcells set), are equal to the sum of cells which will be covered if all nodes have maximum sensing radius (ie Allcells set). As SaveFull Paper
2012Huff, RR;Induction of chondrogenesis from MSCs by BMP7 and TGFB1 in low oxygen tension: expression of superficial zone proteinThesisThe IRC received the sample as whole bone marrow, purchased commercially (AllCells Inc.). To isolate the MSCs, nucleated cells were recovered from a 25mL sample using a Ficoll- SaveFull Paper
2012Sunderic, K;Effects of Mild Heating on the Osteogenic Differentiation of Human Mesenchymal Stem Cells during InflammationThesisHuman bone marrow explanted from the iliac crest of a 24-year old donor was purchased from AllCells, LLC (Berkeley, CA). Human mesenchymal stem cells were enriched using the SaveFull Paper
2012Sangokoya, CO;MicroRNA Function in Cellular Stress ResponseThesisHbAA CD34+ cells (ALLCells) and HbSS CD34+ cells were purified from blood collected from apheresis with donor consent according to Duke IRB protocol. Mononuclear cells were separated by Ficoll-Hypaque density gradient, and CD34+ cells were enriched bFull Paper
2012McNamara, LA;HIV-1 Infection of Hematopoietic Progenitor Cells.ThesisWe obtained pre-existing umbilical cord blood lacking subject identifiers after scheduled cesarean section procedures. We obtained fresh whole bone marrow aspirates from a commercial source (AllCells Ltd.). We prepared BMMCs and UCB mononuclear cellsFull Paper
2012Lowe, RM;Investigation into the role of novel interferon lambdas in the treatment of viral disease, primarily hepatitis CThesis+L-glutamine and 100ng/mL Macrophage colony-stimulating factor (M-CSF) and incubated for four days to differentiate into macrophages. Mast cells were derived from cord blood mononuclear cells (AllCells), cells were cultured in Iscove’s modified DulbeFull Paper
2012David, G;Le locus 1q32: susceptibilité aux maladies inflammatoires de l'intestin et rôles biologiques de C1orf106 et KIF21BThesispréparations d’ARN ont été conservées à -80°C. Les ARN des cellules primaires CD4+, CD8+, CD14+ et CD19+ proviennent de la compagnie Allcells (Californie, États-Unis)Full Paper
2012Lin, X;Novel Factors in Myeloproliferative Neoplasms-The Role of NFIB and miRNA Deregulation in Hematopoietic Cell Proliferation and DifferentiationThesisJAK2V617F mutation was detected in 7 out of 9 specimens by reverse transcription/PCR (AmpliTaq Gold) and directly sequencing JAK2 sequences of 1849 nucleotides amplified. Normal bone marrow CD34+ cells were purchased from AllCells. To isolate CD34+ cFull Paper
2012Budhraja, A;Molecular mechanism of apigenin induced apoptosis in leukemiaThesisNormalperipheral blood mononuclear cells were obtained from AllCells (Emeryville, CA, USA)and were maintained in RPMI 1640 supplemented with 10% FBS. Dr. Ruth Craig( SaveFull Paper
2012Severson, EBS;Developing a Novel In-Vitro Neuroinflammation Model for Huntington's DiseaseThesisHuman BM aspirates were purchased from Lonza and AllCells. Density gradient centrifugation (Ficoll-Hypaque) was used to isolate mononuclear BM-MSC from three donors (AC1, SaveFull Paper
2012Mahmoud, AEM;Investigating the biochemical activity of some flavonoids, coumarins and modified gold-nanoparticles as anti-breast-cancer agentsThesisAllcells were incubated for 2 days at 37 C under 5 % CO2. When the cells reached 50%confluent, the old media was removed and the adherent cells were first washed by IXversene. SaveFull Paper
2012Suh, SS;A study of microRNAs associated with multiple myeloma pathogenesis and microRNAs/TP53 feedback circuit in human cancers, multiple myeloma and glioblastoma multiformeThesiswith monoclonal mouse anti-human CD138 antibodies using the AutoMACs automated separation system (Miltenyi-Biotec Three of the six healthy PCs were obtained from ALLCELLS, SaveFull Paper
2012Berk, E;Dendritic cells regulate the induction of effector and memory CD8+ t cellsThesisSW480 and SW620 (ATCC, Manassas, VA) were grown in IMDM+10% FBS, while HUVECs (AllCells, Emeryville, CA) were cultured in HUVEC complete media (Basal media supplemented with HUVEC stimulatory supplement; AllCells). All were washed, reseeded at 20,000Full Paper
2012Peters, HLC;Characteristics of amyloid precursor-like protein 2 (APLP2) in pancreatic cancer and Ewing's sarcomaThesisAllcells and cellular debris were pelleted by centrifugation at 2,000 xg at 4C for 5 min. Thesupernatant was discarded and pellets containing 5 x 106 cells were resuspended in 100/jL SaveFull Paper
2012Chung, TY;Experimental Beam Studies of Plasma-generated Species Interaction with Polymeric Materials and BiomoleculesThesisThe basic protocol is shown in Figure 3.6. Heparinized fresh human whole blood was acquired from AllCells (Emeryville, CA, USA) and used within 6 h of collection. Samples were prepared by spotting serial diluted lipid A solution on UV/ozone-cleaned sFull Paper
2012Hung, KC;Developing peptide and antibody-mimetic ligands for the cell surface receptor beta2AR and DC-SIGNThesisSallusto and Lanzavecchia, 1994), except that hPBMCs were from HLA-A2+ patients (AllCells, LLC.). iDCs were treated with M3H4-18-HA, stained with anti-HA antibody, and analyzed by either flow cytometry or microscopy same as 293T cells, except that thFull Paper
2012Simonds, EF;Single-Cell Deep Profiling of Immune Signaling and Drug Responses in Normal and Malignant Human HematopoiesisThesisobtained from a tissue bank at Princess Margaret Hospital (Toronto, ON), courtesy of Dr. Mark D. Minden. Cryopreserved healthy BMMCs were purchased from AllCells, Inc. (Emeryville, CA). All human samples were obtained with informed consent in compliaFull Paper
2012Pardee, TS;Gomes, E;Jennings-Gee, J;Caudell, D;Gmeiner, WH;Unique dual targeting of thymidylate synthase and topoisomerase1 by FdUMP[10] results in high efficacy against AML and low toxicityBlood22362039Human cell lines, primary patient samples, or normal human HSCs were thawed and incubated in Stem SpanSFEM media supplemented with Stem Span CC100 cytokine cocktail (StemCell Technologies) for 24 hours with the indicated drug. Cells were then placedFull Paper
2012Zeiger, AS;Loe, FC;Li, R;Raghunath, M;Van Vliet, KJ;Macromolecular crowding directs extracellular matrix organization and mesenchymal stem cell behaviorPloS one22649562ganization correlate with an increase in proliferation and a decrease in motility of MSCs. Materials and Methods Cell Culture Human bone-marrow derived mesenchymal stromal or “stem” cells (MSCs; ReachBio Seattle, WA) were expanded until passage 4-6,Full Paper
2012Jolibois-Quinot, R;Quantitative correlations among human mesenchymal stem cell mechanical properties and biological functionThesisAdult mesenchymal stem cells (referred later as aMSCs) were purchased from commercial sources: ReachBio human bone marrow mesenchymal stem cells - lot number 0090408 (referred later as RB1 - ReachBio LLC, Seattle, WA), and Poietics" human mesenchymalFull Paper
2012Maloney, JM;Chemomechanics of attached and suspended cellsThesisI have also been lucky to work with fine coauthors that dependably contributed honest and top-notch data and ideas: Christopher Bruce and Emily Walton (MIT), Franziska Lautenschläger and Jochen Guck (University of Cambridge), Dessy Nikova (MIT), EmerFull Paper
2011Xu, B;Roos, JL;Dexheimer, P;Boone, B;Plummer, B;Levy, S;Gogos, JA;Karayiorgou, M;Exome sequencing supports a de novo mutational paradigm for schizophreniaNat. Genet.21822266BX, JAG and MK designed the study, interpreted the data and prepared the manuscript; BX developed the analysis pipeline and had the primary role in analysis and validation of sequence data; JLR collected the samples and was the primary clinician on tFull Paper
2011Wood, AM;Van Bortle, K;Ramos, E;Takenaka, N;Rohrbaugh, M;Jones, BC;Jones, KC;Corces, VG;Regulation of chromatin organization and inducible gene expression by a Drosophila insulatorMol. Cell21981916Chromatin immunoprecipitation was performed as described (Bushey et al., 2009). To generate sequencing libraries ChIP DNA was prepared for adaptor ligation by end repair (‘End-It DNA End Repair Kit’ - Epicentre Cat# ER0720) and addition of ‘A’ base tFull Paper
2011Jänne, PA;Boss, DS;Camidge, DR;Britten, CD;Engelman, JA;Garon, EB;Guo, F;Wong, S;Liang, J;Letrent, S;Millham, R;Taylor, I;Eckhardt, SG;Schellens, JH;Phase I dose-escalation study of the pan-HER inhibitor, PF299804, in patients with advanced malignant solid tumorsClin. Cancer Res.21220471Immunohistochemistry analysis was performed by QualTek (Newtown, PA) and was automated throughout using a TechMate 500 or TechMate 1000 (BioTek Solutions/Ventana Medical Systems). Antibodies used were: pERK 1/2 clone 20G11 (Cell Signaling Cat # 4376)Full Paper
2011Ahmad, I;Morton, JP;Singh, LB;Radulescu, SM;β-Catenin activation synergizes with PTEN loss to cause bladder cancer formationOncogeneinvasive bladder carcinoma. We next looked at human bladder UCC using a tissue microarray of 80 cases, 60 UCC (transitional cell carcinomas) and 20 benign controls (Folio Biosciences, Columbus, OH, USA). Using the histoscoreFull Paper
2011Teichert, AE;Elalieh, H;Elias, PM;Welsh, J;Bikle, DD;Overexpression of hedgehog signaling is associated with epidermal tumor formation in vitamin D receptor-null miceJ. Invest. Dermatol.21814234ffinity-purified, biotinylated goat anti-rabbit IgG, followed by ABC-peroxidase reagent, both purchased from Vector (Burlingame, CA). Peroxidase activity was detected with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followedFull Paper
2011Kelly, RK;Olson, DL;Sun, Y;Wen, D;Wortham, KA;Antognetti, G;Cheung, AE;Orozco, OE;Yang, L;Bailly, V;Sanicola, M;An antibody-cytotoxic conjugate, BIIB015, is a new targeted therapy for Cripto positive tumoursEur. J. Cancer214589842.3. Immunohistochemistry. Human breast, colon, lung and ovarian formalin-fixed paraffin-embedded tumour tissue arrays (Imgenex, San Diego, CA, Folio Biosciences, Columbus, OH and Zymed, San Francisco, CA) were stained as described previouslyFull Paper
2011Ahmad, I;Singh, LB;Foth, M;Morris, CA;Taketo, MM;Wu, XR;Leung, HY;Sansom, OJ;Iwata, T;K-Ras and β-catenin mutations cooperate with Fgfr3 mutations in mice to promote tumorigenesis in the skin and lung, but not in the bladderDis Model Mech21504907Finally, we have examined the ERK-MAPK signaling pathway in human UCC by using a tissue microarray (TMA) that contains 60 UCC and 20 benign controls (Folio Biosciences, OH). Using the Histoscore technique, we have quantified the staining intensitiesFull Paper
2011Rokkam, D;Lafemina, MJ;Lee, JW;Matthay, MA;Frank, JA;Claudin-4 levels are associated with intact alveolar fluid clearance in human lungsAm. J. Pathol.21741940controls. Additional normal lung tissue specimens (n = 3) were also obtained (US Biomax, Rockville, MD; Folio Biosciences, Columbus, OH) and immunostained for claudin-4, as previously described. Histology Scoring. TheFull Paper
2011Kent, BN;Salichos, L;Gibbons, JG;Rokas, A;Newton, IL;Clark, ME;Bordenstein, SR;Complete bacteriophage transfer in a bacterial endosymbiont (Wolbachia) determined by targeted genome captureGenome Biol Evol21292630Genomic DNA was extracted from ∼200 abdomens of the B-Wolbachia infected N. vitripennis 4.9 using the QIAGEN DNeasy Kit insect tissue protocol. DNA was treated with 100 mg/ml RNaseA and incubated at room temperature for 2 min. The resulting DNA was qFull Paper
2011Jiang, Y;Lu, J;Peatman, E;Kucuktas, H;Liu, S;Wang, S;Sun, F;Liu, Z;A pilot study for channel catfish whole genome sequencing and de novo assemblyBMC Genomics22192763The culture supernatant was decanted and the block was inverted and tapped gently on paper towels to remove remaining liquid. BAC DNA was isolated using the Perfectprep™ BAC 96 kit (Eppendorf North America, Westbury, NY) according to the manufacturerFull Paper
2011Pitts, RJ;Rinker, DC;Jones, PL;Rokas, A;Zwiebel, LJ;Transcriptome profiling of chemosensory appendages in the malaria vector Anopheles gambiae reveals tissue- and sex-specific signatures of odor codingBMC Genomics21619637We thank the following individuals: Zhen Li for mosquito rearing; Travis Clark, Chelsea Baker and the Vanderbilt Genomics Shared Resource for sample preparation and Illumina sequencing; Shawn Levy and the Genomic Services Lab at HudsonAlpha InstituteFull Paper
2011Liu, S;Zhou, Z;Lu, J;Sun, F;Wang, S;Liu, H;Jiang, Y;Kucuktas, H;Kaltenboeck, L;Peatman, E;Liu, Z;Generation of genome-scale gene-associated SNPs in catfish for the construction of a high-density SNP arrayBMC Genomics21255432;... sing the RNeasy plus Mini Kit (Qiagen, Valencia, CA, USA) with DNase I (Invitrogen, USA) treatment following the manufacturer's protocol. Illumina sequencing Sequencing was conducted commercially in HudsonAlpha Genomic Services Lab (Huntsville,Full Paper
2011Weiss, L;Bernstein, S;Jones, R;Amunugama, R;Krizman, D;Jebailey, L;Almogi-Hazan, O;Hazan, O;Yekhtin, Z;Yachtin, J;Shiner, R;Reibstein, I;Triche, E;Slavin, S;Or, R;Barnea, ER;Preimplantation factor (PIF) analog prevents type I diabetes mellitus (TIDM) development by preserving pancreatic function in NOD miceEndocrine21424847Department of Bone Marrow Transplantation and Cancer Immunotherapy, Hadassah University Hospital Ein Kerem, Hebrew University, Jerusalem, Israel Lola Weiss, Osnat Hazan, Janna Yachtin, Reut Shiner, Israel Reibstein & Reuven Or QualTek Molecular LaborFull Paper
2011Pawar, H;Kashyap, MK;Sahasrabuddhe, NA;Renuse, S;Harsha, HC;Kumar, P;Sharma, J;Kandasamy, K;Marimuthu, A;Nair, B;Rajagopalan, S;Maharudraiah, J;Premalatha, CS;Kumar, KV;Vijayakumar, M;Chaerkady, R;Prasad, TS;Kumar, RV;Kumar, RV;Pandey, A;Quantitative tissue proteomics of esophageal squamous cell carcinoma for novel biomarker discoveryCancer Biol. Ther.21743296The ESCCs varied from tumor grade I to grade III from patients in the age group of 39-79 y. A second group of tissue microarrays obtained from FolioBio (Columbus, OH) consisted of 40 ESCC tissues with matched adjacent normal esophageal squamous epithFull Paper
2011Bunting, RA;Duffy, KE;Lamb, RJ;San Mateo, LR;Smalley, K;Raymond, H;Liu, X;Petley, T;Fisher, J;Beck, H;Flavell, RA;Alexopoulou, L;Ward, CK;Novel antagonist antibody to TLR3 blocks poly(I:C)-induced inflammation in vivo and in vitroCell. Immunol.21092943Mouse tissues (pancreas, inguinal and mesenteric lymph node, brain, liver, heart, trachea, colon, kidneys, lungs, and spleen) from three C57BL/6 and three TLR3KO mice were fixed in 10% neutral buffered formalin and processed for immunohistochemistryFull Paper
2011Trejos, AL;Jayaraman, S;Patel, RV;Naish, MD;Schlachta, CM;Force sensing in natural orifice transluminal endoscopic surgerySurg Endosc20559663allowing the flexible shaft to bend freely. The shaft and the wires were covered with heat-shrink thin-wall tubing (0.26 mm wall thickness, model Q-150K 1/8-02-QB481N- 25; Qualtek Electronics). The 2.8-mm instruments with theFull Paper
2011Karkera, J;Steiner, H;Li, W;Skradski, V;Moser, PL;Riethdorf, S;Reddy, M;Puchalski, T;Safer, K;Prabhakar, U;Pantel, K;Qi, M;Culig, Z;The anti-interleukin-6 antibody siltuximab down-regulates genes implicated in tumorigenesis in prostate cancer patients from a phase I studyProstate21321981PSA, IL‐6R, alpha‐methylacyl‐CoA racemase (AMACR), AR, and Chromogranin A were tested in tumor tissue samples at Qualtek Laboratories (Goleta, CA). Steam heat‐induced epitope retrieval (SHIER) method was used and immunohistochemical procedure was perFull Paper
2011Lynch, FC;A method for following patients with retrievable inferior vena cava filters: results and lessons learned from the first 1,100 patientsJ Vasc Interv Radiol21903414Full Paper
2011Shaw, CM;Scorza, LB;Waybill, PN;Singh, H;Lynch, FC;Optional vena cava filter use in the elderly populationJ Vasc Interv Radiol21530308Full Paper
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2011Maxwell, SA;Cherry, EM;Bayless, KJ;Akt, 14-3-3ζ, and vimentin mediate a drug-resistant invasive phenotype in diffuse large B-cell lymphomaLeuk. Lymphoma21323512Preparation of DLBCL tissue extracts for Western blotting Normal and DLBCL lymphatic tissues were pro- cured through Folio Biosciences (Foliobio.com). Frozen tissue was ground to a powder with a pestle in a mortar while immersed in liquid nitrogenFull Paper
2011Farris, MH;Duffy, C;Findlay, RH;Olson, JB;Streptomyces scopuliridis sp. nov., a bacteriocin-producing soil streptomyceteInt. J. Syst. Evol. Microbiol.20870885; sequencing facility. Genomic DNA isolated from strain RB72 T using the method of Bollet et al. (1991) was sent to the HudsonAlpha Genomic Services Lab (Huntsville, AL) for Illumina Genome Analyser IIx sequencing. 16S rRNAFull Paper
2011Lee, LF;Axtell, R;Tu, GH;Logronio, K;Dilley, J;Yu, J;Rickert, M;Han, B;Evering, W;Walker, MG;Shi, J;de Jong, BA;Killestein, J;Polman, CH;Steinman, L;Lin, JC;IL-7 promotes T(H)1 development and serum IL-7 predicts clinical response to interferon-β in multiple sclerosisScience translational medicine21795588Human mononuclear cells were prepared from the buffy coats of samples from healthy adult donors (Stanford Blood Center) or from cord blood purchased from AllCells on Ficoll-Paque gradients. CD4+ T cells were isolated by magnetic bead depletion of CD1Full Paper
2011Topp, MS;Kufer, P;Gökbuget, N;Goebeler, M;Klinger, M;Neumann, S;Horst, HA;Raff, T;Viardot, A;Schmid, M;Stelljes, M;Schaich, M;Degenhard, E;Köhne-Volland, R;Brüggemann, M;Ottmann, O;Pfeifer, H;Burmeister, T;Nagorsen, D;Schmidt, M;Lutterbuese, R;Reinhardt, C;Baeuerle, PA;Kneba, M;Einsele, H;Riethmüller, G;Hoelzer, D;Zugmaier, G;Bargou, RC;Targeted therapy with the T-cell-engaging antibody blinatumomab of chemotherapy-refractory minimal residual disease in B-lineage acute lymphoblastic leukemia patients results in high response rate and prolonged leukemia-free survivalJournal of clinical oncology : official journal of the American Society of Clinical Oncology21576633Shown is the analysis of DNA fragmentation in B-lineage ALLcells after 48 hours of culture in serum-free medium alone. In this DNA fragmentation assay, intact chromatin and SaveFull Paper
2011Yoneda, A;Kurokawa, T;A sensitive sandwich ELISA to measure (1→ 3)-β-d-glucan levels in bloodJournal of immunological methods2009). 2.7. Measurement of plasma β-glucan. For the measurements of β-glucan level in human plasma, we used commercially available β-glucan-positive human plasma samples purchased from Discovery Life Sciences, IncFull Paper
2011Mayer, JA;Pham, T;Wong, KL;Scoggin, J;Sales, EV;FISH-based determination of HER2 status in circulating tumor cells isolated with the microfluidic CEE™ platformCancer geneticsPeripheral blood was collected under appropriate third-party institutional review board-approved protocols (ConversantBIO, Huntsville, AL; BioOptions, Fullerton, CA) and delivered to Biocept's CLIA/CAP accredited laboratoryFull Paper
2011Lundgren, JA;Matsushima, K;Lynch, FC;Frankel, H;Cooney, RN;Angiographic embolization of nonvariceal upper gastrointestinal bleeding: predictors of clinical failureJ Trauma21610434Full Paper
2011Balazs, AB;Chen, J;Hong, CM;Rao, DS;Yang, L;Baltimore, D;Antibody-based protection against HIV infection by vectored immunoprophylaxisNature22139420Human peripheral mononuclear blood cells (AllCells) were thawed from −80 C, expanded in RPMI medium supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin mix( SaveFull Paper
2011Sigal, A;Kim, JT;Balazs, AB;Dekel, E;Mayo, A;Milo, R;Baltimore, D;Cell-to-cell spread of HIV permits ongoing replication despite antiretroviral therapyNature21849975Anonymous PBMCs or peripheral blood samples were provided by AllCells (PBMCs) or the UCLA Center for AIDS Research (CFAR) Virology Core Lab (peripheral blood). For whole blood, PBMCs were purified by Ficoll gradient using standard techniques. PurifieFull Paper
2011Doyon, Y;Vo, TD;Mendel, MC;Greenberg, SG;Wang, J;Xia, DF;Miller, JC;Urnov, FD;Gregory, PD;Holmes, MC;Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architecturesNature methods21131970K562 cells were transfected using the 96-well Nucleofector Kit SF (Lonza) per manufacturer's recommendations. PBMCs (AllCells) were received on ice, centrifuged at 1,000g for 5 min at room temperature (22 °C) and changed to pre-warmed RPMI medium supFull Paper
2011Chou, BK;Mali, P;Huang, X;Ye, Z;Dowey, SN;Resar, LM;Zou, C;Zhang, YA;Tong, J;Cheng, L;Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signaturesCell research21243013f Hematology in Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, Maryland, USA 5Cell Therapy Center, Xuanwu Hospital and Capital Medical University, Beijing, China 6AllCells LLC, Emeryville, California, USA *The JohFull Paper
2011Lu, SJ;Li, F;Yin, H;Feng, Q;Kimbrel, EA;Hahm, E;Thon, JN;Wang, W;Italiano, JE;Cho, J;Lanza, R;Platelets generated from human embryonic stem cells are functional in vitro and in the microcirculation of living miceCell research21221130versity of Illinois-Chicago review board. Informed consent was provided according to the Declaration of Helsinki. In some experiments, human blood samples were also obtained from a commercial source (AllCells, Emeryville, CA, USA). For hESC-PLTs, culFull Paper
2011Kikushige, Y;Ishikawa, F;Miyamoto, T;Shima, T;Urata, S;Yoshimoto, G;Mori, Y;Iino, T;Yamauchi, T;Eto, T;Niiro, H;Iwasaki, H;Takenaka, K;Akashi, K;Self-renewing hematopoietic stem cell is the primary target in pathogenesis of human chronic lymphocytic leukemiaCancer cell21840488Human age-matched adult bone marrow and peripheral blood cells were obtained from healthy donors or purchased from AllCells Inc. (Emeryville, CA, USA). Informed consent was obtained from all patients and controls in accordance with the Helsinki DeclaFull Paper
2011Panuganti, S;Towards large-scale production of platelets for transfusion based on ex vivo expansion of megakaryocytes from hematopoietic stem cellsThesisCultures were initiated in T-flasks with previously frozen mPB CD34-selected cells (AllCells; Berkeley, CA or Fred Hutchinson Cancer Research Center; Seattle, WA) and maintained at a concentration of 100,000 to 300,000 cells/ml in X-VIVO 20 (Lonza; WFull Paper
2011Carter, CC;McNamara, LA;Onafuwa-Nuga, A;Shackleton, M;Riddell, J;Bixby, D;Savona, MR;Morrison, SJ;Collins, KL;HIV-1 utilizes the CXCR4 chemokine receptor to infect multipotent hematopoietic stem and progenitor cellsCell host & microbe21402361We obtained pre-existing umbilical cord blood lacking subject identifiers after scheduled cesarean section procedures. We obtained fresh whole bone marrow aspirates from a commercial source (AllCells Ltd.). We prepared BMMCs and UCB mononuclear cellsFull Paper
2011Matsuyama, H;Suzuki, HI;Nishimori, H;Noguchi, M;Yao, T;Komatsu, N;Mano, H;Sugimoto, K;Miyazono, K;miR-135b mediates NPM-ALK-driven oncogenicity and renders IL-17-producing immunophenotype to anaplastic large cell lymphomaBlood22042699Many transformed lymphoma cells show immune-phenotypes resembling the corresponding normal lymphocytes, which gives a guide for proper diagnosis and presents promising Save;Human normal peripheral blood pan T lymphocytes were purchased from AllCellFull Paper
2011Aguila, JR;Liao, W;Yang, J;Avila, C;Hagag, N;Senzel, L;Ma, Y;SALL4 is a robust stimulator for the expansion of hematopoietic stem cellsBlood21602528Normal human bone marrow CD34 + cells were purchased (AllCells) and single-cell suspensions were generated in StemSpan SFEM (StemCell Technologies) for 24 hours. Next, 10 5 SaveFull Paper
2011Iancu-Rubin, C;Gajzer, D;Tripodi, J;Najfeld, V;Gordon, RE;Hoffman, R;Atweh, GF;Down-regulation of stathmin expression is required for megakaryocyte maturation and platelet productionBlood21364187Human MKs were generated in liquid culture from peripheral blood (PB)-derived CD34 + cells purchased from AllCells and suspended in Iscove modified Dulbecco medium (Invitrogen) SaveFull Paper
2011Liu, ZJ;Italiano, J;Ferrer-Marin, F;Gutti, R;Bailey, M;Poterjoy, B;Rimsza, L;Sola-Visner, M;Developmental differences in megakaryocytopoiesis are associated with up-regulated TPO signaling through mTOR and elevated GATA-1 levels in neonatal megakaryocytesBlood21304100Human umbilical CB-CD34+ cells were purchased from AllCells. G-CSF-mobilized adult peripheral blood (PB) CD34+ cells were purchased from the Fred Hutchinson Cancer Institute (Seattle, WA). CD34+ cells from either source were cultured in serum-free meFull Paper
2011Adams, GN;LaRusch, GA;Stavrou, E;Zhou, Y;Nieman, MT;Jacobs, GH;Cui, Y;Lu, Y;Jain, MK;Mahdi, F;Shariat-Madar, Z;Okada, Y;D'Alecy, LG;Schmaier, AH;Murine prolylcarboxypeptidase depletion induces vascular dysfunction with hypertension and faster arterial thrombosisBlood21297000HUVECs were purchased from AllCells and grown on gelatin-coated plates in CSC media (Cells Systems). Cells were grown to near-confluence in 6-well plates and transfected with Lipofectamine 2000 reagent (Invitrogen) in OPTI-MEM media (Invitrogen) overFull Paper
2011Hu, K;Yu, J;Suknuntha, K;Tian, S;Montgomery, K;Choi, KD;Stewart, R;Thomson, JA;Slukvin, II;Efficient generation of transgene-free induced pluripotent stem cells from normal and neoplastic bone marrow and cord blood mononuclear cellsBlood21296996Frozen CB mononuclear cells were obtained from AllCells. BM mononuclear cells from normal donors and from a patient with CML in the chronic phase were purchased from AllCells. SaveFull Paper
2011Karp, JE;Smith, BD;Resar, LS;Greer, JM;Blackford, A;Zhao, M;Moton-Nelson, D;Alino, K;Levis, MJ;Gore, SD;Joseph, B;Carraway, H;McDevitt, MA;Bagain, L;Mackey, K;Briel, J;Doyle, LA;Wright, JJ;Rudek, MA;Phase 1 and pharmacokinetic study of bolus-infusion flavopiridol followed by cytosine arabinoside and mitoxantrone for acute leukemiasBlood21239698Flavopiridol is a protein bound, cytotoxic, cyclin-dependent kinase inhibitor. Flavopiridol given by 1-hour bolus at 50 mg/m 2 daily 3 times followed by cytosine arabinoside and SaveFull Paper
2011Huang, X;Gschweng, E;Van Handel, B;Cheng, D;Mikkola, HK;Witte, ON;Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cellsBlood21163928The analyzed samples include H1 hESCs, day 7 and 15 H1 EB cells, and a mixture of 3 adult bone marrow CD34 + cells (AllCells). The data were analyzed with SDS RQ Manager 1.2 ( SaveFull Paper
2011Lugthart, S;Figueroa, ME;Bindels, E;Skrabanek, L;Valk, PJ;Li, Y;Meyer, S;Erpelinck-Verschueren, C;Greally, J;Löwenberg, B;Melnick, A;Delwel, R;Aberrant DNA hypermethylation signature in acute myeloid leukemia directed by EVI1Blood20855866healthy donors, 4 of which were acquired from the Translational Trials Development and Support Laboratory, Cincinnati Children's Hospital, and 4 of which were purchased from Allcells SaveFull Paper
2011Mack, AA;Kroboth, S;Rajesh, D;Wang, WB;Generation of induced pluripotent stem cells from CD34+ cells across blood drawn from multiple donors with non-integrating episomal vectorsPloS one22132178a permit number or approval ID since mouse is not a protected species. Processing whole blood samples Peripheral (PB.1 and PB.2) and cord (CB.1 and CB.2) blood-derived CD34+ cells were obtained from AllCells (Emeryville, CA USA). Blood collections wFull Paper
2011Moorefield, EC;McKee, EE;Solchaga, L;Orlando, G;Yoo, JJ;Walker, S;Furth, ME;Bishop, CE;Cloned, CD117 selected human amniotic fluid stem cells are capable of modulating the immune responsePloS one22046303lates for Elispot (Millipore, Billerica, MA) were coated with human interferon-γ (IFN-γ) capture antibody (2G1; Endogen, Rockford, IL). Peripheral blood mononuclear cells (PBMCs) were acquired from AllCells (Emeryville, CA), activated with 5 µg/ml PHFull Paper
2011Corbin, AS;Agarwal, A;Loriaux, M;Cortes, J;Deininger, MW;Druker, BJ;Human chronic myeloid leukemia stem cells are insensitive to imatinib despite inhibition of BCR-ABL activityThe Journal of clinical investigation21157039Imatinib therapy, which targets the oncogene product BCR-ABL, has transformed chronic myeloid leukemia (CML) from a life-threatening disease into a chronic condition. Most patients, SaveFull Paper
2011Rowe, DD;Secreted factors from human umbilical cord blood cells protect oligodendrocytes from ischemic insultThesisthat prevented OL-HUCB cell contact but was permeable to media and soluble factors. Cryopreserved HUCB cells (ALLCELLS, Emeryville, CA) were rapidly thawed, washed, pelleted to remove the cryopreservatives and re-suspended in 10 ml DMEM with glucoseFull Paper
2011Zhou, L;McMahon, C;Bhagat, T;Alencar, C;Yu, Y;Fazzari, M;Sohal, D;Heuck, C;Gundabolu, K;Ng, C;Mo, Y;Shen, W;Wickrema, A;Kong, G;Friedman, E;Sokol, L;Mantzaris, I;Mantzaris, G;Pellagatti, A;Boultwood, J;Platanias, LC;Steidl, U;Yan, L;Yingling, JM;Lahn, MM;List, A;Bitzer, M;Verma, A;Reduced SMAD7 leads to overactivation of TGF-beta signaling in MDS that can be reversed by a specific inhibitor of TGF-beta receptor I kinaseCancer research21189329Bone marrow CD34+ cells from various normal donors were also obtained from AllCells (Emeryville, CA). K562 and HS-5 cell lines were purchased from ATCC. Erythropoietin and Transforming growth factor-β1 (TGF-β1) were bought from R&D Systems (MinneapolFull Paper
2011Nicolaides, TP;Li, H;Solomon, DA;Hariono, S;Hashizume, R;Barkovich, K;Baker, SJ;Paugh, BS;Jones, C;Forshew, T;Hindley, GF;Hodgson, JG;Kim, JS;Rowitch, DH;Weiss, WA;Waldman, TA;James, CD;Targeted therapy for BRAFV600E malignant astrocytomaClinical cancer research : an official journal of the American Association for Cancer Research22038996Normal human astrocytes (NHA) were obtained from Clonetics and AllCells. All cell sources were authenticated through DNA fingerprinting using the Promega Powerplex platform. SaveFull Paper
2011Kurtzberg, LS;Roth, S;Krumbholz, R;Crawford, J;Bormann, C;Dunham, S;Yao, M;Rouleau, C;Bagley, RG;Yu, XJ;Wang, F;Schmid, SM;Lavoie, EJ;Teicher, BA;Genz-644282, a novel non-camptothecin topoisomerase I inhibitor for cancer treatmentClinical cancer research : an official journal of the American Association for Cancer Research21415217Viable human bone marrow cells were purchased as frozen vials from AllCells, LLC., via StemCell Technologies (#ABM009). They were prepared with a DNase treatment (#07900; SaveFull Paper
2011Arastu-Kapur, S;Anderl, JL;Kraus, M;Parlati, F;Shenk, KD;Lee, SJ;Muchamuel, T;Bennett, MK;Driessen, C;Ball, AJ;Kirk, CJ;Nonproteasomal targets of the proteasome inhibitors bortezomib and carfilzomib: a link to clinical adverse eventsClinical cancer research : an official journal of the American Association for Cancer Research21364033Purpose: Bortezomib (Velcade), a dipeptide boronate 20S proteasome inhibitor and an approved treatment option for multiple myeloma, is associated with a treatment-emergent, painful SaveFull Paper
2011Chao, MP;Alizadeh, AA;Tang, C;Jan, M;Weissman-Tsukamoto, R;Zhao, F;Park, CY;Weissman, IL;Majeti, R;Therapeutic antibody targeting of CD47 eliminates human acute lymphoblastic leukemiaCancer research21177380Human normal bone marrow (NBM) cells were purchased from AllCells Inc. Human ALL samples were obtained from patients at the Stanford University Medical Center, with informed SaveFull Paper
2011Pang, WW;Price, EA;Sahoo, D;Beerman, I;Maloney, WJ;Rossi, DJ;Schrier, SL;Weissman, IL;Human bone marrow hematopoietic stem cells are increased in frequency and myeloid-biased with ageProceedings of the National Academy of Sciences of the United States of America22123971In the human hematopoietic system, aging is associated with decreased bone marrow cellularity, decreased adaptive immune system function, and increased incidence of anemia and Save;Normal young human bone marrow mononuclear cells were purchased froFull Paper
2011Jan, M;Chao, MP;Cha, AC;Alizadeh, AA;Gentles, AJ;Weissman, IL;Majeti, R;Prospective separation of normal and leukemic stem cells based on differential expression of TIM3, a human acute myeloid leukemia stem cell markerProceedings of the National Academy of Sciences of the United States of America21383193Normal human bone marrow mononuclear cells were purchased from AllCells. Human AML samples were obtained from patients at the Stanford Medical Center with informed consent, SaveFull Paper
2011Sahler, J;Bernard, JJ;Spinelli, SL;Blumberg, N;Phipps, RP;The Feverfew plant-derived compound, parthenolide enhances platelet production and attenuates platelet activation through NF-κB inhibitionThrombosis research21272923Adapted from previous procedures [4], human CD34+ cord blood cells were purchased from Allcells (Emeryville, CA) and first plated at 2.5 × 10 5 cells/mL in IMDM supplemented with SaveFull Paper
2011Nahimana, A;Aubry, D;Lagopoulos, L;Greaney, P;Attinger, A;Demotz, S;Dawson, KM;Schapira, M;Tschopp, J;Dupuis, M;Duchosal, MA;A novel potent Fas agonist for selective depletion of tumor cells in hematopoietic transplantsBlood cancer journal22829095lture and analysis conditions Human CD34+ cells from cord blood (CB), mobilized peripheral blood (mPB) or bone marrow (BM) were obtained unpurified (mPB) or already purified (CB, mPB, BM) from either AllCells, LLC (San Mateo, CA, USA), or purified usFull Paper
2011Hart, S;Goh, KC;Novotny-Diermayr, V;Tan, YC;Madan, B;Amalini, C;Ong, LC;Kheng, B;Cheong, A;Zhou, J;Chng, WJ;Wood, JM;Pacritinib (SB1518), a JAK2/FLT3 inhibitor for the treatment of acute myeloid leukemiaBlood cancer journal22829080Primary cells, either peripheral blood mononuclear cells or bone marrow mononuclear cells from AML patients were obtained from AllCells (Emeryville, CA, USA) and ProteoGenex ( SaveFull Paper
2011Nagao, R;Ashihara, E;Kimura, S;Strovel, JW;Yao, H;Takeuchi, M;Tanaka, R;Hayashi, Y;Hirai, H;Padia, J;Strand, K;Maekawa, T;Growth inhibition of imatinib-resistant CML cells with the T315I mutation and hypoxia-adaptation by AV65--a novel Wnt/β-catenin signaling inhibitorCancer letters21906872Bone marrow (BM) mononuclear cells obtained from CML patients and healthy volunteers (ALLCells, Emeryville, CA) were plated in duplicate in MethoCult H4434 Classic (StemCell Technologies Inc, Vancouver, Canada) and cultured at 37 °C in 5% CO2. AfterFull Paper
2011Gao, N;Budhraja, A;Cheng, S;Liu, EH;Chen, J;Yang, Z;Chen, D;Zhang, Z;Shi, X;Phenethyl isothiocyanate exhibits antileukemic activity in vitro and in vivo by inactivation of Akt and activation of JNK pathwaysCell death & disease21472003Fresh normal peripheral blood mononuclear cells were purchased from Allcells (Emeryvill, CA, USA). After washing and enumerating, cells were suspended at 8 × 10 5 /ml before SaveFull Paper
2011Yang, L;Wang, H;Kornblau, SM;Graber, DA;Zhang, N;Matthews, JA;Wang, M;Weber, DM;Thomas, SK;Shah, JJ;Zhang, L;Lu, G;Zhao, M;Muddasani, R;Yoo, SY;Baggerly, KA;Orlowski, RZ;Evidence of a role for the novel zinc-finger transcription factor ZKSCAN3 in modulating Cyclin D2 expression in multiple myelomaOncogene21057542The current studies on deidentified aliquots were performed according to an Institutional Review Board-approved protocol. Normal human CD19+ B-cell total RNA was from Miltenyi Biotec, Inc., and plasma cell cDNA was from AllCells LLC. (Berkeley, CA)Full Paper
2011Yu, X;McCarthy, PJ;Lim, HJ;Iempridee, T;Kraus, RJ;Gorlen, DA;Mertz, JE;The ZIIR element of the Epstein-Barr virus BZLF1 promoter plays a central role in establishment and maintenance of viral latencyJournal of virology21389123Fresh human primary B cells from peripheral blood of healthy donors were purchased from AllCells (Emeryville, CA). The B cells were infected the day they arrived by incubation at 4C SaveFull Paper
2011Sagawa, M;Shimizu, T;Fukushima, N;Kinoshita, Y;Ohizumi, I;Uno, S;Kikuchi, Y;Ikeda, Y;Yamada-Okabe, H;Kizaki, M;A new disulfide-linked dimer of a single-chain antibody fragment against human CD47 induces apoptosis in lymphoid malignant cells via the hypoxia inducible factor-1α pathwayCancer science21401803CD47 belongs to the immunoglobulin superfamily and is associated with β‐integrins. Recently it was reported that CD47 ligation rapidly induces apoptosis in B‐chronic lymphocytic SaveFull Paper
2011Takeuchi, M;Ashihara, E;Yamazaki, Y;Kimura, S;Nakagawa, Y;Tanaka, R;Yao, H;Nagao, R;Hayashi, Y;Hirai, H;Maekawa, T;Rakicidin A effectively induces apoptosis in hypoxia adapted Bcr-Abl positive leukemic cellsCancer science21166958Bone marrow cells from two normal volunteers and CML patients in the chronic phase before imatinib treatment were purchased from AllCells Inc. (Emeryville, CA, USA). Thawed SaveFull Paper
2011Su, WR;Zhang, QZ;Shi, SH;Nguyen, AL;Le, AD;Human gingiva-derived mesenchymal stromal cells attenuate contact hypersensitivity via prostaglandin E2-dependent mechanismsStem cells (Dayton, Ohio)21987520CD14+ cells were negatively selected from human peripheral blood mononuclear cells (PBMCs) (AllCells LLC, Emeryville, CA, http://www.allcells.com) using the MACS CD14 MicroBeads (Monocyte Isolation Kit, Miltenyi Biotec, Auburn, CA, http://www.miltenyFull Paper
2011Bissels, U;Wild, S;Tomiuk, S;Hafner, M;Scheel, H;Mihailovic, A;Choi, YH;Tuschl, T;Bosio, A;Combined characterization of microRNA and mRNA profiles delineates early differentiation pathways of CD133+ and CD34+ hematopoietic stem and progenitor cellsStem cells (Dayton, Ohio)21394831tenyi Biotec) using the negative fraction of the first separation. The gating of the separated cells was performed according to ISHAGE. CD133+ cells from mobilized peripheral blood were obtained from AllCells LLC. RNA Sources Total RNA was isolatedFull Paper
2011Mendelson, A;Frank, E;Allred, C;Jones, E;Chen, M;Zhao, W;Mao, JJ;Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cells in vitroFASEB journal : official publication of the Federation of American Societies for Experimental Biology21746864Human bone marrow MSCs (AllCells, Emeryville, CA, USA), human ASCs, and human SSCs were isolated from fresh adult donor samples using previously established methods (27-31). MSCs were purified from bone marrow using the RosetteSep Human Mesenchymal SFull Paper
2011Gao, N;Budhraja, A;Cheng, S;Liu, EH;Huang, C;Chen, J;Yang, Z;Chen, D;Zhang, Z;Shi, X;Interruption of the MEK/ERK signaling cascade promotes dihydroartemisinin-induced apoptosis in vitro and in vivoApoptosis : an international journal on programmed cell death21336837Fresh normal peripheral blood mononuclear cells were purchased from Allcells (Emeryville, CA, USA). After washing and enumerating as described for mononuclear cells, cells were SaveFull Paper
2011Zhou, L;Opalinska, J;Sohal, D;Yu, Y;Mo, Y;Bhagat, T;Abdel-Wahab, O;Fazzari, M;Figueroa, M;Alencar, C;Zhang, J;Kambhampati, S;Parmar, S;Nischal, S;Hueck, C;Suzuki, M;Freidman, E;Pellagatti, A;Boultwood, J;Steidl, U;Sauthararajah, Y;Yajnik, V;McMahon, C;Gore, SD;Platanias, LC;Levine, R;Melnick, A;Wickrema, A;Greally, JM;Verma, A;Aberrant epigenetic and genetic marks are seen in myelodysplastic leukocytes and reveal Dock4 as a candidate pathogenic gene on chromosome 7qThe Journal of biological chemistry21532034106 CD34+ cells (AllCells) were thawed, cultured for 2 h, resuspended in 100 μl of human CD34+ Nucleofection solution (Amaxa), then transferred into cuvettes, and electroporated using program U008. CD34+ cells were collected and cultured in 24-well pFull Paper
2011Froelich, S;Tai, A;Kennedy, K;Zubair, A;Wang, P;Pseudotyping lentiviral vectors with aura virus envelope glycoproteins for DC-SIGN-mediated transduction of dendritic cellsHuman gene therapy21452926Peripheral blood mononuclear cells (PBMCs) from healthy human donors were purchased from AllCells. PBMCs were allowed to adhere for 2 hr on non-tissue culture-treated 24-well plates and washed extensively. Adherent monocytes were plated at a densityFull Paper
2011Jiang, X;Christopherson, GT;Mao, HQ;The effect of nanofibre surface amine density and conjugate structure on the adhesion and proliferation of human haematopoietic progenitor cellsInterface focus23050077Frozen human UCB CD133+ cells were purchased from AllCells, LLC (Emeryville, CA, USA). The CD133+ purity in the post-thawed HSPCs was determined by flow cytometry to be 97 per cent and the viability was determined by Trypan blue exclusion assay to beFull Paper
2011Zhu, J;Shimizu, E;Zhang, X;Partridge, NC;Qin, L;EGFR signaling suppresses osteoblast differentiation and inhibits expression of master osteoblastic transcription factors Runx2 and OsterixJournal of cellular biochemistry21381079The epidermal growth factor receptor (EGFR) and its ligands regulate key processes of cell biology, such as proliferation, survival, differentiation, migration, and tumorigenesis. We SaveFull Paper
2011Khoury, M;Drake, A;Chen, Q;Dong, D;Leskov, I;Fragoso, MF;Li, Y;Iliopoulou, BP;Hwang, W;Lodish, HF;Chen, J;Mesenchymal stem cells secreting angiopoietin-like-5 support efficient expansion of human hematopoietic stem cells without compromising their repopulating potentialStem cells and development21142526Purified human CD133 + cord blood cells were purchased from AllCells. Alternatively, umbilical cord blood was obtained from the National Disease Research Interchange or the SaveFull Paper
2011Hong, L;Zhang, G;Sultana, H;Yu, Y;Wei, Z;The effects of 17-β estradiol on enhancing proliferation of human bone marrow mesenchymal stromal cells in vitroStem cells and development20735179Fresh human bone marrow donated by 12 healthy donors (6 males and 6 females, average age = 27.4 ± 6.1) were purchased (Allcells, Emeryville, CA). Mononuclear cells were isolated using density gradient centrifugation (Histopaque-1.077; Sigma, St. LouiFull Paper
2011Kunisato, A;Wakatsuki, M;Shinba, H;Ota, T;Ishida, I;Nagao, K;Direct generation of induced pluripotent stem cells from human nonmobilized bloodStem cells and development20497033BM and PB MNCs were obtained from Allcells (ABM007F and PB003F each). Frozen BM MNCs purchased from Allcells were collected from a 25-year-old male donor (lot#BM1705), a SaveFull Paper
2011Basu, J;Genheimer, CW;Sangha, N;Quinlan, SF;Guthrie, KI;Kelley, R;Ilagan, RM;Jain, D;Bertram, T;Ludlow, JW;Organ specific regenerative markers in peri-organ adipose: kidneyLipids in health and disease21957910CD34+ GCSF mobilized/non-mobilized peripheral blood mononuclear cell cDNA was purchased from AllCells LLC. Fetal and adult hepatocyte cDNA and keratinocyte cDNA were SaveFull Paper
2011Gupta, MS;Cooper, ES;Nicoll, SB;Transforming growth factor-beta 3 stimulates cartilage matrix elaboration by human marrow-derived stromal cells encapsulated in photocrosslinked carboxymethylcellulose hydrogels: potential for nucleus pulposus replacementTissue engineering. Part A21707438hMSCs were isolated from the bone marrow of a 37-year-old man (AllCells, Emeryville, CA) using enriched density centrifugation. Briefly, an hMSC Enrichment Cocktail (StemCell Technologies, Vancouver, BC, Canada)44 was added to the bone marrow and incFull Paper
2011McWhirter, J;Noguchi, H;Gompper, G;Deformation and clustering of red blood cells in microcapillary flowsSoft MatterTrajectories are shown in a co-moving frame, ie relative to the center-of-mass of allcells. Solid and dashed lines represent the cm of RBCs and their periodic images, respectively. SaveFull Paper
2011Vanella, L;Kim, DH;Sodhi, K;Barbagallo, I;Burgess, AP;Falck, JR;Schwartzman, ML;Abraham, NG;Crosstalk between EET and HO-1 downregulates Bach1 and adipogenic marker expression in mesenchymal stem cell derived adipocytesProstaglandins & other lipid mediators21821145Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α-MEM, Invitrogen, Carlsbad, CA) supplemented with 10% heat inactivated fetal boviFull Paper
2011MacEneaney, OJ;Connick, E;DeSouza, CA;Effects of HIV-1 gp120 and protease inhibitors on apoptotic susceptibility of CD34+ hematopoietic progenitor cellsJournal of acquired immune deficiency syndromes (1999)21233630Freshly-isolated CD34+ cells from healthy G-CSF-treated human donors (n=3) were obtained from an independent supplier (Allcells, LLC, Berkeley, CA). Donors tested negative for HIV-1, hepatitis B and hepatitis C. Viability of cells determined by trypaFull Paper
2011He, JQ;Vu, DM;Hunt, G;Chugh, A;Bhatnagar, A;Bolli, R;Human cardiac stem cells isolated from atrial appendages stably express c-kitPloS one22140461Human bone marrow cells were purchased from AllCells (Emeryville, CA). Total RNA of human carotid artery was bought from Agilent Technologies (Santa Clara, CA). Cells were SaveFull Paper
2011Kobayashi, DT;Olson, RJ;Sly, L;Swanson, CJ;Chung, B;Naryshkin, N;Narasimhan, J;Bhattacharyya, A;Mullenix, M;Chen, KS;Utility of survival motor neuron ELISA for spinal muscular atrophy clinical and preclinical analysesPloS one21904622For the dilutional linearity experiment, six lots of control PBMCs were purchased from AllCells for further assay testing. After thawing and lysis, PBMCs were serially diluted 4-fold and SaveFull Paper
2011Froelich, S;Tai, A;Kennedy, K;Zubair, A;Wang, P;Virus-receptor mediated transduction of dendritic cells by lentiviruses enveloped with glycoproteins derived from Semliki Forest virusPloS one21738680th 293T.DCSIGN cells for 8 hrs before changing to fresh D10 medium. Transduction of human PBMC-derived DCs Peripheral blood mononuclear cells (PBMC) from two healthy human donors were purchased from AllCells (Emeryville, CA). PBMC were differentiateFull Paper
2011Danner, R;Chaudhari, SN;Rosenberger, J;Surls, J;Richie, TL;Brumeanu, TD;Casares, S;Expression of HLA class II molecules in humanized NOD.Rag1KO.IL2RgcKO mice is critical for development and function of human T and B cellsPloS one21611197) Tg.NOD.Rag1KO.IL2RγcKO (referred as DRAG) mice were maintained in the pathogen-free WRAIR Veterinary Medicine Facility. Infusion of mice with HSC Umbilical cord bloods (UCB) were purchased from AllCells (Emeryville,CA) and Promocell (Heidelberg, GFull Paper
2011Drake, AC;Khoury, M;Leskov, I;Iliopoulou, BP;Fragoso, M;Lodish, H;Chen, J;Human CD34+ CD133+ hematopoietic stem cells cultured with growth factors including Angptl5 efficiently engraft adult NOD-SCID Il2rγ-/- (NSG) micePloS one21559522rch. The MIT committee on animal care (MIT CAC) approved the research as part of animal protocol 0310-027-13. Cell isolation and culture Purified CD133+ human umbilical cord HSCs were purchased from AllCells (California) or National Disease ResearchFull Paper
2011Burridge, PW;Thompson, S;Millrod, MA;Weinberg, S;Yuan, X;Peters, A;Mahairaki, V;Koliatsos, VE;Tung, L;Zambidis, ET;A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variabilityPloS one21494607For generation of the non-viral CD34+ cord blood-derived hiPSC lines (CBiPSC6.2, CBiPSC6.11, CBiPSC6.13 and CBiPSC19.11), 0.5-1×106 human CD34+ cord blood cells (AllCells, Emeryville, CA, http://www.allcells.com) were expanded in StemSpan-SFEMFull Paper
2011Martellini, JA;Cole, AL;Svoboda, P;Stuchlik, O;Chen, LM;Chai, KX;Gangrade, BK;Sørensen, OE;Pohl, J;Cole, AM;HIV-1 enhancing effect of prostatic acid phosphatase peptides is reduced in human seminal plasmaPloS one21283773obtained from the National Institutes of Health AIDS Research and Reference Reagent Program (Germantown, MD, USA), and Peripheral Blood Mononuclear Cells (PBMCs) were obtained from healthy donors by AllCells, LLC (Emeryville, CA, USA). TZM-bl cells wFull Paper
2011Gupta, A;Le, A;Belinka, BA;Kachlany, SC;In vitro synergism between LFA-1 targeting leukotoxin (Leukothera™) and standard chemotherapeutic agents in leukemia cellsLeukemia research21664691and maintained in RPMI 1640 medium with 10% fetal bovine serum (Invitrogen, Carlsbad, CA) at 37 °C, 5% CO2. 2.2. Primary cells Frozen primary human cells were purchased from AllCells, LLC. (Emeryville, CA). Viability of these primary cells was 90%Full Paper
2011Bouchez, LC;Boitano, AE;de Lichtervelde, L;Romeo, R;Cooke, MP;Schultz, PG;Small-molecule regulators of human stem cell self-renewalChembiochem : a European journal of chemical biology21381174screen: The assay was performed on purified human CD34 + cells from granulocyte colony-stimulating factor-mobilized peripheral blood from normal donors (supplied by AllCells, SaveFull Paper
2011Fukumoto, R;Kiang, JG;Geldanamycin analog 17-DMAG limits apoptosis in human peripheral blood cells by inhibition of p53 activation and its interaction with heat-shock protein 90 kDa after exposure to ionizing radiationRadiation research21663398Exposure to ionizing radiation induces p53, and its inhibition improves mouse survival. We tested the effect of 17-dimethylamino-ethylamino-17-demethoxygeldanamycin (17-DMAG) on SaveFull Paper
2011Pogosova-Agadjanyan, EL;Fan, W;Georges, GE;Schwartz, JL;Kepler, CM;Lee, H;Suchanek, AL;Cronk, MR;Brumbaugh, A;Engel, JH;Yukawa, M;Zhao, LP;Heimfeld, S;Stirewalt, DL;Identification of radiation-induced expression changes in nonimmortalized human T cellsRadiation research21268710Peripheral blood samples were collected from healthy volunteer donors at the Fred Hutchinson Cancer Research Center (FHCRC) or purchased from a commercial vendor (AllCells, Emeryville, CA). All samples were obtained under Institutional Review Board (Full Paper
2011Bagley, RG;Kurtzberg, L;Rouleau, C;Yao, M;Teicher, BA;Erufosine, an alkylphosphocholine, with differential toxicity to human cancer cells and bone marrow cellsCancer chemotherapy and pharmacology21526352and compounds were added to each well of a 6-well plate in a final volume of 3 mls; RPMI8226 multiple myeloma cells and compounds were added to 3 mls Methocult medium (AllCells SaveFull Paper
2011Hong, L;Krishnamachari, Y;Seabold, D;Joshi, V;Schneider, G;Salem, AK;Intracellular release of 17-β estradiol from cationic polyamidoamine dendrimer surface-modified poly (lactic-co-glycolic acid) microparticles improves osteogenic differentiation of human mesenchymal stromal cellsTissue engineering. Part C, Methods20883116and solvents were purchased from Sigma. All the solvents, including ethyl acetate and dichloromethane (DCM) and acetonitrile, were of high performance liquid chromatography (HPLC) analytical grade. Fresh human bone marrow was purchased from Allcells.Full Paper
2011McNeish, J;Mathieu, A;Dempsey, B;Book Review: Bringing stem cell science to the classroomsRegenerative Medicinefrom a small biotechnology company, ReachBio (Seatttle, WA, USA). ReachBio is a contract of clients", so education is clearly a passion at ReachBio. As much of the focus of the text is SaveFull Paper
2011Nelson, DM;Joseph, B;Hillion, J;Segal, J;Karp, JE;Resar, LM;Flavopiridol induces BCL-2 expression and represses oncogenic transcription factors in leukemic blasts from adults with refractory acute myeloid leukemiaLeukemia & lymphoma21728742from the peripheral blood blasts were compared to gene expression in CD34+ hematopoietic stem cells (HSCs); mRNA levels from the HSCs were arbitrarily assigned a value of 1.0. HSC RNA was obtained from a commercial source (ALLCELLS, CA, USA)Full Paper
2011Ma, K;Fox, L;Shi, G;Shen, J;Liu, Q;Pappas, JD;Cheng, J;Qu, T;Generation of neural stem cell-like cells from bone marrow-derived human mesenchymal stem cellsNeurological research22196762The hMSCs from four donors were obtained from AllCells LLC (Emeryville, CA, USA) and Cambrex Bioscience (Walkersville, MD, USA). These hMSCs are negative for surface markers associated with hematopoietic cells (e.g. CD11b, CD33, CD34, and CD133) andFull Paper
2011Wang, DY;Chang, CW;Lagacé, RE;Oldroyd, NJ;Hennessy, LK;Development and validation of the AmpFℓSTR® Identifiler® Direct PCR Amplification Kit: a multiplex assay for the direct amplification of single-source samplesJournal of forensic sciences21418220the performance of the Identifiler Direct Kit on buccal samples deposited on Indicating FTA cards, white blood cells (WBCs) from three individuals were purchased from AllCells, SaveFull Paper
2011Shah, BS;Mao, JJ;Labeling of mesenchymal stem cells with bioconjugated quantum dotsMethods in molecular biology (Clifton, N.J.)21153373Bone marrow samples from healthy human donors (AllCells, Berkeley, CA) 2. RosetteSepTM (Stem Cell Technologies, Vancouver, BC, Canada) 3. 35-mm petri dishes (Falcon, Becton Dickinson Labware, Franklin Lakes, NJ) 4. DMEM: Dulbecco’s Modified Eagle’Full Paper
2011Li, Z;Chen, J;In vitro functional study of miR-126 in leukemiaMethods in molecular biology (Clifton, N.J.)20931398Genomic DNA template isolated from human normal bone marrow mononuclear cells purchased from AllCells (Emeryville, CA). 3. MSCVpuro (Clontech, Mountain View, CA) is a retroviral vector for cloning and gene expression. 4. AML1-ETO fusion gene in a vFull Paper
2011Kanjevac, T;Milovanovic, M;Volarevic, V;Arsenijevic, N;Fluoride release from glass ionomer cements correlates with the necrotic death of human dental pulp stem cellsSerbian Journal of Experimental and Clinical ResearchHuman SHEDs were purchesed from AllCells (Emeryville, California USA). The cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 10% FBS, 100 IU/mL penicillin G and 100 μg/mL streptomycinFull Paper
2011Bassan, R;Rohatiner, A;Lerede, T;Carter, M;Di Bona, E;Pogliani, E;Rossi, G;Fabris, P;Morandi, S;Lambertenghi-Deliliers, G;Casula, P;Vespignani, M;Lister, T;Barbui, T;Early Application of Anthracyclines in Adult Acute Lymphoblastic Leukemia (ALL)Haematology and Blood Transfusion / Hämatologie und BluttransfusionHowever, because IDR resistance may be dose-dependent, as ascertained by in vitro studies on MDRl+ ALLcells [17], increaseddose IDR may find a place in the treatment of patients SaveFull Paper
2011Larson, R;Dodge, R;Szatrowski, T;Frankel, S;Results of Recent Clinical Trials in Acute Lymphoblastic Leukemia by the Cancer and Leukemia Group BHaematology and Blood Transfusion / Hämatologie und BluttransfusionRapid depletion of asparagine results in selective killing of ALLcells since normal cells are able to synthesize asparagine. Because asparaginase is not markedly myelosuppressive, it is SaveFull Paper
2011Bissels, U;Combined analysis of microRNA and mRNA signatures in human hematopoietic stem and progenitor cells using a novel microarray quantification systemThesisGranulocyte-colony stimulating factor (G-CSF) mobilized peripheral blood was obtained from healthy donors after informed consent (Allcells, Berkley, USA) and CD133+ cells were SaveFull Paper
2011Milewski, ME;The Angiogenic effect of Erythropoietin on Stem Cells In-VitroThesisHUVEC cells (p-10) were obtained from Allcells, LLC (Emeryville, CA). The cells were removed from the -80°C and placed in a 37°C water bath until thawed. 10 ml of HUVEC media (Allcells, Emeryville, CA) was added to the cells and centrifuged for 5 minFull Paper
2011Stewart, HRA;A novel reporter system where gene knockdown is used to assay intercellular siRNA transferThesisMesenchymal stem cells were harvested from bone marrow aspirates purchased from Lonza and AllCells. To isolate human MSCs, nucleated cells were recovered from the 10ml bone marrow aspirates with a density gradient (Ficoll-Paque) and resuspended in coFull Paper
2011KC, P;Proteomic Analysis of Human Mesenchymal Stem Cell Aging during serial sub-cultureThesisHuman bone marrow was purchased from AllCells, LLC, Emeryvill, CA from a 28 years old donor. Human mesenchymal stem cells were isolated following RosetteSep method and expanded in culture using CFU-F assay. Fluorescence-activated cell sorting (FACS)Full Paper
2011Stosich, MS;Micro-CT evaluation of MSC/HUVEC/HSC-mediated angiogenesis in large PCL-βTCP constructs implanted in nude miceThesisFor in vivo study, fresh bone marrow samples of anonymous adult male donors were purchased from AllCells (Berkeley, CA), and used to isolate human mesenchymal stem cells ( SaveFull Paper
2011Pang, WW;Park, CY;Greenberg, PL;Separation of normal and aberrant hematopoietic stem cells in myelodysplastic syndrome by CD69 expressionThesisHuman Samples. Normal young human bone marrow mononuclear cells were purchased from AllCells, Inc. (Emeryville, CA, USA). Normal young and old human bone marrow samples were obtained from hematologically normal donors at the Stanford Medical Center wFull Paper
2011Ramos, JH;Phenotypic and molecular characterization of a human adult bone marrow committed T cell progenitorThesisHuman mononuclear cells obtained from fresh, normal adult bone marrow were purchased from AllCells, LLC. (Emeryville, CA). Donors were between the ages of 18 and 55 years, 110 lbs, in good health, and screened for HIV, Hepatitis B and Hepatitis C befFull Paper
2011Thacker, SG;Type I Interferons and the Development of Impaired Vascular Function and Repair in Human and Murine LupusThesislU/ml for 6 hours, followed by RNA harvesting. Similar experiments were performed with a more purified EPC population which was directly obtained from control bone marrow. In brief, CD133+ cells obtained from healthy control bone marrows (AllCells,Full Paper
2011Panas, MW;The identification and elimination of immunosuppressive Mycobacterium bovis BCG genes to create novel rBCG vectors that generate increased CD8+ T cell responsesThesisThe delivery of an allelic exchange substrate to allcells within a mycobacterial population will be accomplished through specializedtransduction using the temperature sensitive phage SaveFull Paper
2011Hermann, RJ;Investigation of Ikaros-dependent transcriptional regulation of novel VPAC1 splice variants during epigenetic transformationThesisIn order to identify which PBMC were the cell source for smaller VPAC1 isoforms, purified samples of different cell populations that compose the collective PBMC pool had to be analyzed. cDNA from purified peripheral blood cell populations was purchasFull Paper
2011Furman, PA;Murakami, E;Niu, C;Lam, AM;Espiritu, C;Bansal, S;Bao, H;Tolstykh, T;Micolochick Steuer, H;Keilman, M;Zennou, V;Bourne, N;Veselenak, RL;Chang, W;Ross, BS;Du, J;Otto, MJ;Sofia, MJ;Activity and the metabolic activation pathway of the potent and selective hepatitis C virus pronucleotide inhibitor PSI-353661Antiviral research21600932The human bone marrow stem cell toxicity assay was performed by Reachbio (Seattle, WA). Clonogenic progenitor cells of the erythroid (CFU-E and BFU-E) and myeloid (CFU-GM) SaveFull Paper
2011Lam, AM;Espiritu, C;Murakami, E;Zennou, V;Bansal, S;Micolochick Steuer, HM;Niu, C;Keilman, M;Bao, H;Bourne, N;Veselenak, RL;Reddy, PG;Chang, W;Du, J;Nagarathnam, D;Sofia, MJ;Otto, MJ;Furman, PA;Inhibition of hepatitis C virus replicon RNA synthesis by PSI-352938, a cyclic phosphate prodrug of β-D-2'-deoxy-2'-α-fluoro-2'-β-C-methylguanosineAntimicrobial agents and chemotherapy21444700marrow (Lonza, Walkersville, MD) were performed by Reachbio (Seattle, WA) using a semisolid We also thank Emer Clarke at ReachBio LLC (Seattle, WA) for performing human bone SaveFull Paper
2011Veeranki, S;Duan, X;Panchanathan, R;Liu, H;Choubey, D;IFI16 protein mediates the anti-inflammatory actions of the type-I interferons through suppression of activation of caspase-1 by inflammasomesPloS one22046441y the AIM2 and NLRP3 inflammasomes. Materials and Methods Cells and Treatments Frozen human peripheral blood mononuclear cells (PBMCs), purified CD14+ monocytes and CD19+ B cells were purchased from ReachBio (Seattle, WA). THP-1 cells were purchasedFull Paper
2011Maier, MS;Setting occupational exposure limits for unstudied pharmaceutical intermediates using an in vitro parallelogram approachToxicology mechanisms and methods20156007The mean ± 1 SD was calculated for triplicate cultures. The laboratory (ReachBio LLC, Seattle, Washington) was blinded to the identify of the test compounds and docetaxel served as the positive control, with blank and vehicle as negative controlsFull Paper
2011Lautenschlager, F;Cell compliance: cytoskeletal origin and importance for cellular function.ThesisHuman MSCs (hMSCs) were investigated over the time they needed to double their population 17 times, which corresponds to about eight passages. Cells were obtained from the bone marrow of adult donors (Stem Cell Technologies and ReachBio LLC), isolateFull Paper
2010Yelamanchili, SV;Chaudhuri, AD;Chen, LN;Xiong, H;Fox, HS;MicroRNA-21 dysregulates the expression of MEF2C in neurons in monkey and human SIV/HIV neurological diseaseCell Death Dis21170291miRNA in situ hybridization. Following necropsy, monkey caudate and hippocampus were formalin fixed, and embedded in paraffin, and 5 μm sections cut onto glass slides. The in situ hybridization was performed at Phylogeny Inc. (Columbus, OH, USA) as dFull Paper
2010Maret, D;Gruzglin, E;Sadr, MS;Siu, V;Shan, W;Koch, AW;Seidah, NG;Del Maestro, RF;Colman, DR;Surface expression of precursor N-cadherin promotes tumor cell invasionNeoplasia21170270Melanoma, breast, squamous cell, and prostate carcinoma tissue samples and arrays, in addition to corresponding normal tissue, were obtained from FolioBio (Columbus, OH). Metastatic melanoma arrays were from Imgenex (San Diego, CA). Cell Culture andFull Paper
2010Teichert, A;Elalieh, H;Bikle, D;Disruption of the hedgehog signaling pathway contributes to the hair follicle cycling deficiency in Vdr knockout miceJ. Cell. Physiol.20458748The binding of the antibodies was detected by biotinylated goat anti‐rabbit IgG, followed by ABC‐peroxidase reagent (Vector, Burlingame, CA) and revealed with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followed by hematoxyliFull Paper
2010Kashyap, MK;Harsha, HC;Renuse, S;Pawar, H;Sahasrabuddhe, NA;Kim, MS;Marimuthu, A;Keerthikumar, S;Muthusamy, B;Kandasamy, K;Subbannayya, Y;Prasad, TS;Mahmood, R;Chaerkady, R;Meltzer, SJ;Kumar, RV;Rustgi, AK;Pandey, A;SILAC-based quantitative proteomic approach to identify potential biomarkers from the esophageal squamous cell carcinoma secretomeCancer Biol. Ther.20686364... _id":"553574440","term_text":"ESC96101"}}ESC96101 [/protein/ESC96101]) consisted of 34 cases in duplicate, ranging from differentiation grades I–III from patient's ages 36–71 years. The TMAs from FolioBio (catalog # ARY-HH0091) consisted of 40 ESFull Paper
2010Li, Z;Qi, CF;Shin, DM;Zingone, A;Newbery, HJ;Kovalchuk, AL;Abbott, CM;Morse, HC;Eef1a2 promotes cell growth, inhibits apoptosis and activates JAK/STAT and AKT signaling in mouse plasmacytomasPLoS ONE20505761correction. A tissue microarray of costal biopsies of normal individuals or patients diagnosed with MM was purchased from Folio BioSciences (Columbus, OH). Immunohistochemical studies were detailed previously [27]. MouseFull Paper
2010Shih, DM;Xia, YR;Yu, JM;Lusis, AJ;Temporal and tissue-specific patterns of Pon3 expression in mouse: in situ hybridization analysisAdv. Exp. Med. Biol.20221872Detailed biochemical studies are also necessary to identify the physiological substrate(s) of PON3 in various cells/tissues. Notes. Acknowledgment. We thank Yi-Shou Shi, XuPing Wang, and Phylogeny, Inc. (Columbus, OH) for excellent technical supportFull Paper
2010Hill, LS;Richardson, TL;Profeta, LT;Shaw, TJ;Hintz, CJ;Twining, BS;Lawrenz, E;Myrick, ML;Construction, figures of merit, and testing of a single-cell fluorescence excitation spectroscopy systemRev Sci Instrum20113077The relay box uses a Bud Industries PN series box enclosure (model PN-1341-C) as the base. The circuit consisted of two NTE Electronics solid-state relays (model RS3–1D10–51), two Omron Electronic relays (model G4B-112T-C-U.S.-AC120), one Qualtek EleFull Paper
2010Gentles, AJ;Plevritis, SK;Majeti, R;Alizadeh, AA;Association of a leukemic stem cell gene expression signature with clinical outcomes in acute myeloid leukemiaJAMA21177505Normal human bone marrow mononuclear cells were purchased from AllCells Inc (Emeryville, California), and human cord blood was obtained from Stanford University Medical Center. SaveFull Paper
2010Carter, CC;Onafuwa-Nuga, A;McNamara, LA;Riddell, J;Bixby, D;Savona, MR;Collins, KL;HIV-1 infects multipotent progenitor cells causing cell death and establishing latent cellular reservoirsNature medicine20208541minates all consensus AP-1, NF-AT, NF-κB SP-1 and TATA motifs, but maintains the polyA site found in the R region. Cell culture Fresh whole bone marrow aspirates were obtained commercially (AllCells ltd.). Pre-existing umbilical cord blood lacking pFull Paper
2010Naka, K;Hoshii, T;Muraguchi, T;Tadokoro, Y;Ooshio, T;Kondo, Y;Nakao, S;Motoyama, N;Hirao, A;TGF-beta-FOXO signalling maintains leukaemia-initiating cells in chronic myeloid leukaemiaNature20130650solid medium. Human LICs were purified as CD34+CD38-Lin- cells of bone marrow cells from CML patients (Cureline, Inc. and AllCells). These cells were co-cultured on OP-9 cells for 5 days before transfer to semi-solid medium. Cell cycling was assessedFull Paper
2010Lishko, PV;Botchkina, IL;Fedorenko, A;Kirichok, Y;Acid extrusion from human spermatozoa is mediated by flagellar voltage-gated proton channelCell20144758Frozen normal peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryville, CA), thawed and plated onto sterile coverslips according to the manufacturer instructions. Mouse monoclonal anti-COXIV and anti-CD14 antibody were fromFull Paper
2010Loewer, S;Cabili, MN;Guttman, M;Loh, YH;Thomas, K;Park, IH;Garber, M;Curran, M;Onder, T;Agarwal, S;Manos, PD;Datta, S;Lander, ES;Schlaeger, TM;Daley, GQ;Rinn, JL;Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cellsNature genetics21057500The CD34 + cells used were mobilized peripheral blood CD34+ cells (AllCells, mBP014F). Cells were thawed and cultured as described 21 for two days before RNA isolation. SaveFull Paper
2010Pichiorri, F;Suh, SS;Rocci, A;De Luca, L;Taccioli, C;Santhanam, R;Zhou, W;Benson, DM;Hofmainster, C;Alder, H;Garofalo, M;Di Leva, G;Volinia, S;Lin, HJ;Perrotti, D;Kuehl, M;Aqeilan, RI;Palumbo, A;Croce, CM;Downregulation of p53-inducible microRNAs 192, 194, and 215 impairs the p53/MDM2 autoregulatory loop in multiple myeloma developmentCancer cell20951946IRB-approved procurement protocol (2000C0247) at The Ohio State University and The University of Turin GIMEMA-MM-03-05, N. EUDRACT 2005-004745-33). Three of the five healthy PCs used were purchased from AllCells LLC. (Emeryville, CA)Full Paper
2010Figueroa, ME;Lugthart, S;Li, Y;Erpelinck-Verschueren, C;Deng, X;Christos, PJ;Schifano, E;Booth, J;van Putten, W;Skrabanek, L;Campagne, F;Mazumdar, M;Greally, JM;Valk, PJ;Löwenberg, B;Delwel, R;Melnick, A;DNA methylation signatures identify biologically distinct subtypes in acute myeloid leukemiaCancer cell20060365We hypothesized that DNA methylation distributes into specific patterns in cancer cells, which reflect critical biological differences. We therefore examined the methylation profiles of 344 SaveFull Paper
2010Liu, W;Lee, HW;Liu, Y;Wang, R;Rodgers, GP;Olfactomedin 4 is a novel target gene of retinoic acids and 5-aza-2'-deoxycytidine involved in human myeloid leukemia cell growth, differentiation, and apoptosisBlood20724538Peripheral blood mononuclear cells from AML patients and normal individuals, and neutrophils from normal individuals, were obtained commercially from AllCells. The neutrophils were SaveFull Paper
2010Tatsumi, K;Taatjes, D;Bouchard, B;Trotman, W;Bovill, E;Cell Adhesion Molecule 1 (CADM1), a Novel Venous Thrombosis Risk Factor, Is Ubiquitously Present In Vascular Endothelium and Smooth Muscle CellsBloodImmunoelectron microscopy (IEM) was performed on cultured human umbilical vein endothelial cells (Allcells LLC, Emeryville, CA) using a post embedding procedure with protein-A SaveFull Paper
2010Lin, X;Rice, K;Carroll, M;Costa, F;Kilpivaara, O;Soares, M;Levine, R;Licht, J;Mir-9 Is Aberrantly Expressed In MPN Patients and Accelerates Erythropoietic Cell Growth and DifferentiationBloodTo determine how deregulated expression of miR-9 affected erythropoiesis, we transduced human bone marrow CD34+ cells (AllCells) with empty vector or miR-9 lentivirus, and Save;Abstract 1983 The myeloproliferative neoplasms (MPNs), PFull Paper
2010Campbell, JE;Brummel-Ziedins, KE;Butenas, S;Mann, KG;Cellular regulation of blood coagulation: a model for venous stasisBlood20864579We have adapted the corn-trypsin inhibitor whole-blood model to include EA.hy926 as an endothelium surrogate to evaluate the vascular modulation of blood coagulation initiated by SaveFull Paper
2010Sangokoya, C;Telen, MJ;Chi, JT;microRNA miR-144 modulates oxidative stress tolerance and associates with anemia severity in sickle cell diseaseBlood20709907Normal hemoglobin (HbAA) CD34+ cells (ALLCells) and HbSS CD34+ cells were purified from blood collected from apheresis with donor consent according to Duke Institutional Review Board protocol. Mononuclear cells were separated by Ficoll-Hypaque densitFull Paper
2010Prabhala, RH;Pelluru, D;Fulciniti, M;Prabhala, HK;Nanjappa, P;Song, W;Pai, C;Amin, S;Tai, YT;Richardson, PG;Ghobrial, IM;Treon, SP;Daley, JF;Anderson, KC;Kutok, JL;Munshi, NC;Elevated IL-17 produced by TH17 cells promotes myeloma cell growth and inhibits immune function in multiple myelomaBlood20395418approval by the institutional review board (IRB) at Dana-Farber Cancer Institute. Healthy donor samples were obtained from the blood donor center at Children's Hospital, Boston, MA. Normal donor bone marrow samples were obtained from AllCellsFull Paper
2010Liu, X;Shin, N;Koblish, HK;Yang, G;Wang, Q;Wang, K;Leffet, L;Hansbury, MJ;Thomas, B;Rupar, M;Waeltz, P;Bowman, KJ;Polam, P;Sparks, RB;Yue, EW;Li, Y;Wynn, R;Fridman, JS;Burn, TC;Combs, AP;Newton, RC;Scherle, PA;Selective inhibition of IDO1 effectively regulates mediators of antitumor immunityBlood20197554Bone marrow mononuclear cells of patients with AML were purchased from ALLCELLS and maintained according to the recommended culture conditions. SaveFull Paper
2010Quintás-Cardama, A;Vaddi, K;Liu, P;Manshouri, T;Li, J;Scherle, PA;Caulder, E;Wen, X;Li, Y;Waeltz, P;Rupar, M;Burn, T;Lo, Y;Kelley, J;Covington, M;Shepard, S;Rodgers, JD;Haley, P;Kantarjian, H;Fridman, JS;Verstovsek, S;Preclinical characterization of the selective JAK1/2 inhibitor INCB018424: therapeutic implications for the treatment of myeloproliferative neoplasmsBlood20130243Mononuclear cells were isolated from peripheral blood from patients with PV or normal control persons by centrifugation through Ficoll (Allcells). A total of 2 × 10 5 cells from control or SaveFull Paper
2010Swift, S;Ellison, AR;Kassner, P;McCaffery, I;Rossi, J;Sinclair, AM;Begley, CG;Elliott, S;Absence of functional EpoR expression in human tumor cell linesBlood20124514Erythroid progenitor cells were generated from CD34 + peripheral blood cells mobilized with granulocyte colony-stimulating factor (G-CSF; ALLCELLS) cultured for 6 days in StemSpan SaveFull Paper
2010Driscoll, JJ;Pelluru, D;Lefkimmiatis, K;Fulciniti, M;Prabhala, RH;Greipp, PR;Barlogie, B;Tai, YT;Anderson, KC;Shaughnessy, JD;Annunziata, CM;Munshi, NC;The sumoylation pathway is dysregulated in multiple myeloma and is associated with adverse patient outcomeBlood19965618Fresh, normal, human adult bone marrow was from AllCells, and CD138 + cells were affinity purified by positive selection using Miltenyi CD138 + microbeads (Miltenyi Biotec) according SaveFull Paper
2010Lee, CH;Shah, B;Moioli, EK;Mao, JJ;CTGF directs fibroblast differentiation from human mesenchymal stem/stromal cells and defines connective tissue healing in a rodent injury modelThe Journal of clinical investigation20679726Human MSCs were isolated from fresh whole bone marrow samples of 2 anonymous adult donors (age range, 20-25 years; AllCells) and were exempt from IRB approval. Nonhematopoietic, mononucleated and adherent cells were purified by centrifugation throughFull Paper
2010Chao, MP;Jaiswal, S;Weissman-Tsukamoto, R;Alizadeh, AA;Gentles, AJ;Volkmer, J;Weiskopf, K;Willingham, SB;Raveh, T;Park, CY;Majeti, R;Weissman, IL;Calreticulin is the dominant pro-phagocytic signal on multiple human cancers and is counterbalanced by CD47Science translational medicine21178137Normal human bone marrow mononuclear cells were purchased from AllCells Inc. Normal peripheral blood and human cancer samples were obtained from patients at the Stanford SaveFull Paper
2010Collins, JM;Ayala, P;Desai, TA;Russell, B;Three-dimensional culture with stiff microstructures increases proliferation and slows osteogenic differentiation of human mesenchymal stem cellsSmall (Weinheim an der Bergstrasse, Germany)19943257however, experiments were performed on P2 hMSCs obtained from 2 separate donors. Additionally, some experiments were repeated with P2 hMSCs isolated from whole marrow (AllCells, Berkely, CA) as described previously.[29]Full Paper
2010Cheng, S;Gao, N;Zhang, Z;Chen, G;Budhraja, A;Ke, Z;Son, YO;Wang, X;Luo, J;Shi, X;Quercetin induces tumor-selective apoptosis through downregulation of Mcl-1 and activation of BaxClinical cancer research : an official journal of the American Association for Cancer Research21138867Mononuclear cells were isolated from peripheral blood or bone marrow of leukemia patients or healthy donors were purchased from AllCells, LLC. Mononuclear cells were suspended SaveFull Paper
2010Berkofsky-Fessler, W;Buzzai, M;Kim, MK;Fruchtman, S;Najfeld, V;Min, DJ;Costa, FF;Bischof, JM;Soares, MB;McConnell, MJ;Zhang, W;Levine, R;Gilliland, DG;Calogero, R;Licht, JD;Transcriptional profiling of polycythemia vera identifies gene expression patterns both dependent and independent from the action of JAK2V617FClinical cancer research : an official journal of the American Association for Cancer Research20601445Normal bone marrow CD34 + cells were purchased from Allcells. To isolate CD34 + cells, bone marrow samples were diluted 1:1 in PBS and layered onto Histopaque 1077 (Sigma; SaveFull Paper
2010Marchi, E;Paoluzzi, L;Scotto, L;Seshan, VE;Zain, JM;Zinzani, PL;O'Connor, OA;Pralatrexate is synergistic with the proteasome inhibitor bortezomib in in vitro and in vivo models of T-cell lymphoid malignanciesClinical cancer research : an official journal of the American Association for Cancer Research20501616Peripheral blood mononuclear cells (PBMC) from healthy donors were purchased from Allcells. All cell lines were grown as previously described (21). SaveFull Paper
2010Paoluzzi, L;Scotto, L;Marchi, E;Zain, J;Seshan, VE;O'Connor, OA;Romidepsin and belinostat synergize the antineoplastic effect of bortezomib in mantle cell lymphomaClinical cancer research : an official journal of the American Association for Cancer Research20068080Mononuclear cells from PBMC samples of healthy donors were purchased from Allcells. All cell lines were grown as described previously (12, 13). SaveFull Paper
2010Brunckhorst, MK;Wang, H;Lu, R;Yu, Q;Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesisCancer research20823154Human umbilical vein ECs (HUVEC) and normal human astrocytes (NHA) were from AllCells, Inc., U87MG was obtained from American Type Culture Collection, and U251 was SaveFull Paper
2010Cho, SY;Xu, M;Roboz, J;Lu, M;Mascarenhas, J;Hoffman, R;The effect of CXCL12 processing on CD34+ cell migration in myeloproliferative neoplasmsCancer research20388788Fresh normal marrow was purchased from AllCells LLC. Cord blood (CB) collections were provided by Cord Blood Bank of New York Blood Center. SaveFull Paper
2010Xu, Y;Stamenkovic, I;Yu, Q;CD44 attenuates activation of the hippo signaling pathway and is a prime therapeutic target for glioblastomaCancer research20197461C, expression of endogenous CD44 in a panel of human glioma cells and NHAs (ALLCELLS, Inc.) was determined by Western blotting using anti-CD44 antibody (Santa Cruz). Total SaveFull Paper
2010Hart, S;Goh, K;Tan, Y;Amalini, C;Wood, J;Abstract 2524: SB1518, a novel oral JAK2-FLT3 inhibitor, is efficacious in models of acute myeloid leukemia and primary leukemic cells from patientsCancer ResearchFor progenitor expansion, PBMCs of AML patients (AllCells Inc) were cultured using a medium containing IL-3, IL-6, SCF and FLT3L (StemCell Technologies TB 28440). The expanded SaveFull Paper
2010Santanam, U;Zanesi, N;Efanov, A;Costinean, S;Palamarchuk, A;Hagan, JP;Volinia, S;Alder, H;Rassenti, L;Kipps, T;Croce, CM;Pekarsky, Y;Chronic lymphocytic leukemia modeled in mouse by targeted miR-29 expressionProceedings of the National Academy of Sciences of the United States of America20566844Real-time PCR experiments were carried out using miR-29a, miR-29b, and PXDN assays for real-time PCR (Applied Biosystems) according to the manufacturer's protocol. Control human cord blood CD19+ B cells were purchased from Allcells and LonzaFull Paper
2010Mi, S;Li, Z;Chen, P;He, C;Cao, D;Elkahloun, A;Lu, J;Pelloso, LA;Wunderlich, M;Huang, H;Luo, RT;Sun, M;He, M;Neilly, MB;Zeleznik-Le, NJ;Thirman, MJ;Mulloy, JC;Liu, PP;Rowley, JD;Chen, J;Aberrant overexpression and function of the miR-17-92 cluster in MLL-rearranged acute leukemiaProceedings of the National Academy of Sciences of the United States of America20133587obtained at the time of diagnosis or relapse and with informed consent at the University of Chicago or other hospitals (see characteristics of patients in Table S2). Normal control cells were purchased from AllCells. (Emeryville, CA)Full Paper
2010Wu, PK;Ringeisen, BR;Development of human umbilical vein endothelial cell (HUVEC) and human umbilical vein smooth muscle cell (HUVSMC) branch/stem structures on hydrogel layers via biological laser printing (BioLP)Biofabrication20811126HUVEC were purchased from Allcells, and HUVSMC were purchased from ATCC. Matrigel was purchased from BD Bioscience. The cells were grown in a humidified environment with 5 SaveFull Paper
2010Bhatwadekar, AD;Guerin, EP;Jarajapu, YP;Caballero, S;Sheridan, C;Kent, D;Kennedy, L;Lansang, MC;Ruscetti, FW;Pepine, CJ;Higgins, PJ;Bartelmez, SH;Grant, MB;Transient inhibition of transforming growth factor-beta1 in human diabetic CD34+ cells enhances vascular reparative functionsDiabetes20460428CD34 + cells enriched by Miltenyi positive selection from human cord blood and bone marrow obtained from AllCells (Emeryville, CA) and were >95% pure as assessed by fluorescence SaveFull Paper
2010Lin, TH;Hegen, M;Quadros, E;Nickerson-Nutter, CL;Appell, KC;Cole, AG;Shao, Y;Tam, S;Ohlmeyer, M;Wang, B;Goodwin, DG;Kimble, EF;Quintero, J;Gao, M;Symanowicz, P;Wrocklage, C;Lussier, J;Schelling, SH;Hewet, AG;Xuan, D;Krykbaev, R;Togias, J;Xu, X;Harrison, R;Mansour, T;Collins, M;Clark, JD;Webb, ML;Seidl, KJ;Selective functional inhibition of JAK-3 is sufficient for efficacy in collagen-induced arthritis in miceArthritis and rheumatism20506481Cryopreserved peripheral blood mononuclear cells (PBMCs; AllCells) were cultured in complete RPMI 1640 medium (supplemented with 10% fetal bovine serum, penicillin/streptomycin, 2 mM L-glutamine, and 1 mM sodium pyruvate) plus 10 μg/ml lectin phytoheFull Paper
2010Kodippili, GC;Spector, J;Kang, GE;Liu, H;Wickrema, A;Ritchie, K;Low, PS;Analysis of the kinetics of band 3 diffusion in human erythroblasts during assembly of the erythrocyte membrane skeletonBritish journal of haematology20553270Baxter Healthcare, Inc., Deerfield, IL) from commercially available growth factor mobilized blood samples (AllCells LLC, Emeryville, CA), as described elsewhere (Kang, et al 2008). Briefly, CD34+ cells isolated from growth-factor mobilized peripheralFull Paper
2010Lavelle, D;Saunthararajah, Y;Vaitkus, K;Singh, M;Banzon, V;Phiasivongsva, P;Redkar, S;Kanekal, S;Bearss, D;Shi, C;Inloes, R;DeSimone, J;S110, a novel decitabine dinucleotide, increases fetal hemoglobin levels in baboons (P. anubis)Journal of translational medicine20932295Frozen CD34+ human cells purified from the peripheral blood of mobilized donors were purchased from Allcells, Inc. These cells were cultured in Iscove's media containing 20% fetal SaveFull Paper
2010Yamaza, T;Kentaro, A;Chen, C;Liu, Y;Shi, Y;Gronthos, S;Wang, S;Shi, S;Immunomodulatory properties of stem cells from human exfoliated deciduous teethStem cell research & therapy20504286Healthy bone marrow aspirates from iliac bone and peripheral blood mononuclear cells (PBMNCs) of healthy volunteers were purchased from AllCells (Berkeley, CA, USA). SaveFull Paper
2010Luo, XM;Lei, MY;Feidi, RA;West, AP;Balazs, AB;Bjorkman, PJ;Yang, L;Baltimore, D;Dimeric 2G12 as a potent protection against HIV-1PLoS pathogens21187894ting amounts of monomer or dimer from the separated purified species. Humanized mice, passive transfer, and HIV-1 challenge Frozen human cord blood CD34+ cells from single donors were purchased from AllCells (Emeryville, CA) or Lonza (Basel, SwitzerFull Paper
2010Ellis-Connell, AL;Iempridee, T;Xu, I;Mertz, JE;Cellular microRNAs 200b and 429 regulate the Epstein-Barr virus switch between latency and lytic replicationJournal of virology20668090Raji cell assay as described above. Approximately 1 × 105 GRUs/ml was used to infect 1 × 106 cells/ml of primary blood B cells (AllCells, Emeryville, CA). Samples were harvested at the times indicated in Fig. 2 and processed as described above for ZEFull Paper
2010Li, J;Favata, M;Kelley, JA;Caulder, E;Thomas, B;Wen, X;Sparks, RB;Arvanitis, A;Rogers, JD;Combs, AP;Vaddi, K;Solomon, KA;Scherle, PA;Newton, R;Fridman, JS;INCB16562, a JAK1/2 selective inhibitor, is efficacious against multiple myeloma cells and reverses the protective effects of cytokine and stromal cell supportNeoplasia (New York, N.Y.)20072651Primary bone marrow CD138+ plasma cells from a newly diagnosed MM patient were purchased from Allcells (Emeryville, CA). The cells were cultured in the same medium used for SaveFull Paper
2010Jekle, A;Chhabra, M;Lochner, A;Meier, S;Chow, E;Brandt, M;Sankuratri, S;Cammack, N;Heilek, G;Epitope switching as a novel escape mechanism of HIV to CCR5 monoclonal antibodiesAntimicrobial agents and chemotherapy1999592310% FBS and 1% penicillin-streptomycin. Human peripheral blood mononuclear cells (PBMC) were obtained from AllCells (Emeryville, CA), stimulated for 1 day in PBMC medium (RPMI 1640 medium containing 10% FBS, 1% penicillin-streptomycin, 2 mM l-glutamiFull Paper
2010Culpin, RE;Proctor, SJ;Angus, B;Crosier, S;Anderson, JJ;Mainou-Fowler, T;A 9 series microRNA signature differentiates between germinal centre and activated B-cell-like diffuse large B-cell lymphoma cell linesInternational journal of oncology20596664streptomycin (Gibco), according to the repositories guidelines. Cultures were incubated at 37˚C in a 5% CO2 humidified atmosphere. CD19+ negatively-selected B-cell total RNA (pooled from 3 unrelated donors) served as the normal healthy B-cell controlFull Paper
2010Collins, JM;Goldspink, PH;Russell, B;Migration and proliferation of human mesenchymal stem cells is stimulated by different regions of the mechano-growth factor prohormoneJournal of molecular and cellular cardiology208758252 mM L-glutamine, 100 units/mL penicillin and 100 μg/mL streptomycin. hMSCs were obtained from the Tulane University Center for Gene Therapy or AllCells (Berkeley, CA). Passage 2-3 (P2-P3) hMSCs were used for all experimentsFull Paper
2010Destache, CJ;Belgum, T;Goede, M;Shibata, A;Belshan, MA;Antiretroviral release from poly(DL-lactide-co-glycolide) nanoparticles in miceThe Journal of antimicrobial chemotherapy20729545Frozen elutriated monocytes were obtained from AllCells, LLC (Emeryville, CA, USA). Cells were thawed in a water bath at 37C and counted using Trypan Blue exclusion. Cells (5 × 10 SaveFull Paper
2010Berahovich, RD;Zabel, BA;Penfold, ME;Lewén, S;Wang, Y;Miao, Z;Gan, L;Pereda, J;Dias, J;Slukvin, II;McGrath, KE;Jaen, JC;Schall, TJ;CXCR7 protein is not expressed on human or mouse leukocytesJournal of immunology (Baltimore, Md. : 1950)20889540Human normal bone marrow cells were purchased from AllCells (Emeryville, CA). Human thymocytes were obtained by mechanical disruption of fetal thymus tissue purchased from SaveFull Paper
2010Thacker, SG;Berthier, CC;Mattinzoli, D;Rastaldi, MP;Kretzler, M;Kaplan, MJ;The detrimental effects of IFN-α on vasculogenesis in lupus are mediated by repression of IL-1 pathways: potential role in atherogenesis and renal vascular rarefactionJournal of immunology (Baltimore, Md. : 1950)20805419Similar experiments were performed with EPCs directly obtained from control bone marrow. In brief, CD133+ cells obtained from healthy control bone marrows (AllCells, Emeryville, CA) were thawed following manufacturer’s recommendations and then culturFull Paper
2010Toti, US;Guru, BR;Grill, AE;Panyam, J;Interfacial activity assisted surface functionalization: a novel approach to incorporate maleimide functional groups and cRGD peptide on polymeric nanoparticles for targeted drug deliveryMolecular pharmaceutics20527782methyl tri-chlorosilane were from Sigma-Aldrich (St Louis, MO). RPMI cell media, fetal bovine serum, and trypsin were purchased from Invitrogen (Carlsbad, CA). HUVEC media was supplied by AllCells (Emeryville, CA)Full Paper
2010Medina, RJ;O'Neill, CL;Humphreys, MW;Gardiner, TA;Stitt, AW;Outgrowth endothelial cells: characterization and their potential for reversing ischemic retinopathyInvestigative ophthalmology & visual science20554606of 1 × 107 cells/mL. Population doubling was carefully monitored, and OECs at early passages were used for all experiments. Frozen human cord blood mononuclear cells were purchased (Allcells; Stem Cell Technologies, Emeryville, CA) and plated under tFull Paper
2010Kopher, RA;Penchev, VR;Islam, MS;Hill, KL;Khosla, S;Kaufman, DS;Human embryonic stem cell-derived CD34+ cells function as MSC progenitor cellsBone20601304Mayo Institutional Review Board and obtaining informed consent, or isolated from whole bone marrow (AllCells, Emeryville, CA, USA) using standard methods [50]. BM-MSCs were cultured in MSC media as described above. Adherent cells were selected for duFull Paper
2010Vanella, L;Kim, DH;Asprinio, D;Peterson, SJ;Barbagallo, I;Vanella, A;Goldstein, D;Ikehara, S;Kappas, A;Abraham, NG;HO-1 expression increases mesenchymal stem cell-derived osteoblasts but decreases adipocyte lineageBone19853072Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential SaveFull Paper
2010Kim, DH;Vanella, L;Inoue, K;Burgess, A;Gotlinger, K;Manthati, VL;Koduru, SR;Zeldin, DC;Falck, JR;Schwartzman, ML;Abraham, NG;Epoxyeicosatrienoic acid agonist regulates human mesenchymal stem cell-derived adipocytes through activation of HO-1-pAKT signaling and a decrease in PPARγStem cells and development20412023Frozen bone marrow mononuclear cells were purchased from Allcells (Allcells, Emeryville, CA). After thawing, mononuclear cells were resuspended in an α-minimal essential medium (α SaveFull Paper
2010Fox, LE;Shen, J;Ma, K;Liu, Q;Shi, G;Pappas, GD;Qu, T;Cheng, J;Membrane properties of neuron-like cells generated from adult human bone-marrow-derived mesenchymal stem cellsStem cells and development20394468The hMeSCs from 4 donors were commercially obtained from AllCells LLC and Cambrex Bioscience. The cell lines were negative for surface markers associated with hematopoietic SaveFull Paper
2010Lai, Y;Sun, Y;Skinner, CM;Son, EL;Lu, Z;Tuan, RS;Jilka, RL;Ling, J;Chen, XD;Reconstitution of marrow-derived extracellular matrix ex vivo: a robust culture system for expanding large-scale highly functional human mesenchymal stem cellsStem cells and development19737070Freshly isolated human bone marrow mononuclear cells obtained from 20- to 30-year-old donors were purchased from ALLCELLS (Emeryville, CA), and grown on tissue culture plastic SaveFull Paper
2010McLaughlin, J;Markovtsov, V;Li, H;Wong, S;Gelman, M;Zhu, Y;Franci, C;Lang, D;Pali, E;Lasaga, J;Low, C;Zhao, F;Chang, B;Gururaja, TL;Xu, W;Baluom, M;Sweeny, D;Carroll, D;Sran, A;Thota, S;Parmer, M;Romane, A;Clemens, G;Grossbard, E;Qu, K;Jenkins, Y;Kinoshita, T;Taylor, V;Holland, SJ;Argade, A;Singh, R;Pine, P;Payan, DG;Hitoshi, Y;Preclinical characterization of Aurora kinase inhibitor R763/AS703569 identified through an image-based phenotypic screenJournal of cancer research and clinical oncology19609559Cultured human primary mast cells were established from human umbilical cord blood CD34 + progenitor cells (AllCells, LLC, Berkeley, CA) using the method described in US patent no SaveFull Paper
2010Chen, NK;Tan, SY;Udolph, G;Kon, OL;Insulin expressed from endogenously active glucose-responsive EGR1 promoter in bone marrow mesenchymal stromal cells as diabetes therapyGene therapy20182520HBMMSC (AllCells, Emeryville, CA, USA) cultured overnight in Dulbecco's modified Eagle's medium (DMEM)-3 mM glucose (as the low glucose baseline) were exposed to DMEM-20 mM glucose (high glucose) for 15 or 30 min, and then returned to DMEM-3 mM glucoFull Paper
2010Marchi, E;Pralatrexate sinergizza in vitro ed in vivo con l'inibitore del Proteosoma Bortezomib in modelli pre-clinici di Linfoma Non Hodkin TThesisaggiunto a giorni alterni. Le cellule sono state mantenute in coltura alla concentrazione ideale di 0.5 x 106 cellule/mL e trattate come descritto in precedenti studi pubblicati52 . Le cellule mononucleate del sangue periferico da donatori volontariFull Paper
2010Qureshi, A;Subathra, M;Grey, A;Schey, K;Del Poeta, M;Luberto, C;Role of sphingomyelin synthase in controlling the antimicrobial activity of neutrophils against Cryptococcus neoformansPloS one21203393Approximately 6.4×10 4 fresh human normal peripheral blood-neutrophils per ml (AllCells) were incubated in 124 µl sterile filtered PBS supplemented with 10% pooled human serum SaveFull Paper
2010O'Connell, RM;Balazs, AB;Rao, DS;Kivork, C;Yang, L;Baltimore, D;Lentiviral vector delivery of human interleukin-7 (hIL-7) to human immune system (HIS) mice expands T lymphocyte populationsPloS one207004542×10 7 PBMCs (AllCells) that were first labeled with CFSE. HIS mice were created as described[5]. In brief, 1.5×10 5 highly purified CD34+ CB stem cells (AllCells) were injected into the SaveFull Paper
2010Smith, B;Treadwell, J;Zhang, D;Ly, D;McKinnell, I;Walker, PR;Sikorska, M;Large-scale expression analysis reveals distinct microRNA profiles at different stages of human neurodevelopmentPloS one20559549obtained from ScienCell Research Laboratories (Carlsbad, CA) and were directly pelleted for RNA extraction. Frozen primary human fetal astrocytes (PHAf) from 20-21 week fetuses were received from ALLCELLS (Emeryville, CA) and were cultured in AstrocFull Paper
2010Kachlany, SC;Schwartz, AB;Balashova, NV;Hioe, CE;Tuen, M;Le, A;Kaur, M;Mei, Y;Rao, J;Anti-leukemia activity of a bacterial toxin with natural specificity for LFA-1 on white blood cellsLeukemia research19747730The oral bacterium, Aggregatibacter actinomycetemcomitans, produces a leukotoxin (LtxA) that is specific for white blood cells (WBCs) from humans and Old World primates by SaveFull Paper
2010Medina, RJ;O'Neill, CL;Sweeney, M;Guduric-Fuchs, J;Gardiner, TA;Simpson, DA;Stitt, AW;Molecular analysis of endothelial progenitor cell (EPC) subtypes reveals two distinct cell populations with different identitiesBMC medical genomics20465783l approval from three female volunteer subjects aged 25-35, non-smokers, not receiving any medication and without any clinical diagnosis; while CB-derived mononuclear cells (MNCs) were purchased from AllCells LLC (California, USA). MNCs were isolatedFull Paper
2010Rowe, DD;Leonardo, CC;Hall, AA;Shahaduzzaman, MD;Collier, LA;Willing, AE;Pennypacker, KR;Cord blood administration induces oligodendrocyte survival through alterations in gene expressionBrain research20883670Oligodendrocytes (OLs), the predominant cell type found in cerebral white matter, are essential for structural integrity and proper neural signaling. Very little is known concerning stroke- SaveFull Paper
2010Jin, Q;Alkhatib, B;Cornetta, K;Alkhatib, G;Alternate receptor usage of neuropilin-1 and glucose transporter protein 1 by the human T cell leukemia virus type 1Virology19913864streptomycin sulfate (0.1 mg/ml) in a humidified incubator held at 5% CO2. All cell culture media were purchased from GIBCO. Normal Human Astrocytes (NHA) and their basal medium (with 10% FBS) were purchased from AllCells (AllCells, LLC., Emeryville,Full Paper
2010Walczak-Drzewiecka, A;Ratajewski, M;Pułaski, Ł;Dastych, J;DNA methylation-dependent suppression of HIF1A in an immature hematopoietic cell line HMC-1Biochemical and biophysical research communications19995553cDNAs from BM CD34+, PB CD34+, BM MNCs, PB MNCs and BM MSCs purchased from AllCells (Emeryville, CA, USA) was obtained from a population of positive cells (purity exceeding 95%) collected from five individual donors. The level of cognate cDNA was meaFull Paper
2010Li, W;Hong, L;Hu, L;Magin, RL;Magnetization transfer imaging provides a quantitative measure of chondrogenic differentiation and tissue developmentTissue engineering. Part C, Methods20373975Mesenchymal stem cells (MSCs), isolated from fresh adult human bone marrow obtained from AllCells LLC (Berkeley, CA), were plated at a density of 107 nucleated cells per 100-mm-diameter Petri dish. The cells were incubated in a basic medium of DulbecFull Paper
2010Akers, SM;O'Leary, HA;Minnear, FL;Craig, MD;Vos, JA;Coad, JE;Gibson, LF;VE-cadherin and PECAM-1 enhance ALL migration across brain microvascular endothelial cell monolayersExperimental hematology20470859Positively selected peripheral blood CD19 + B cells from a healthy donor were purchased from AllCells (Emeryville, CA, USA). HBMEC were obtained from Angioproteomie (Boston, MA SaveFull Paper
2010Schuhmann, NK;Pozzoli, O;Sallach, J;Huber, A;Avitabile, D;Perabo, L;Rappl, G;Capogrossi, MC;Hallek, M;Pesce, M;Büning, H;Gene transfer into human cord blood-derived CD34(+) cells by adeno-associated viral vectorsExperimental hematology20447441After thawing, hCD34 + cells (AllCells) were plated and expanded for 3 days as described. Cells were then incubated with rAAV2sc (at a GOI of 1 × 10 4 ) for 18 hours, followed by an SaveFull Paper
2010Lu, M;Zhang, W;Li, Y;Berenzon, D;Wang, X;Wang, J;Mascarenhas, J;Xu, M;Hoffman, R;Interferon-alpha targets JAK2V617F-positive hematopoietic progenitor cells and acts through the p38 MAPK pathwayExperimental hematology20303384Objective Interferon-α (IFNα) therapy leads to hematological remissions and a reduction of the JAK2V617F allele burden in patients with polycythemia vera (PV). In this study, the SaveFull Paper
2010Yourek, G;McCormick, SM;Mao, JJ;Reilly, GC;Shear stress induces osteogenic differentiation of human mesenchymal stem cellsRegenerative medicine20868327Isolated bone marrow (AllCells, Berkeley, CA, USA) from multiple healthy human donors aged between 20 and 26 years (seven male and one female) was enriched for hMSCs using RosetteSep® (StemCell Technologies, Vancouver, BC, Canada). Bone marrow was inFull Paper
2010Barbagallo, I;Vanella, A;Peterson, SJ;Kim, DH;Tibullo, D;Giallongo, C;Vanella, L;Parrinello, N;Palumbo, GA;Di Raimondo, F;Abraham, NG;Asprinio, D;Overexpression of heme oxygenase-1 increases human osteoblast stem cell differentiationJournal of bone and mineral metabolism19924377Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA, USA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential SaveFull Paper
2010Covey, TM;Putta, S;Cesano, A;Single cell network profiling (SCNP): mapping drug and target interactionsAssay and drug development technologies20158439Cryopreserved, Ficoll fractionated, bone marrow samples from healthy individual were purchased from AllCells (Emeryville, CA). Cells from cryogenic storage were thawed in a 37C SaveFull Paper
2010Bingaman, MG;Basu, GD;Golding, TC;Chong, SK;Lassen, AJ;Kindt, TJ;Lipinski, CA;The autophilic anti-CD20 antibody DXL625 displays enhanced potency due to lipid raft-dependent induction of apoptosisAnti-cancer drugs20216307blood lymphocytes (PBLs) were purified from healthy donor samples using standard Ficoll-Plaque separation. Normal human peripheral blood mononuclear cells (PBMCs) were from AllCells (Emeryville, California, USA). CD56-immunomagnetic separation beadsFull Paper
2010Elliott, S;Busse, L;McCaffery, I;Rossi, J;Sinclair, A;Spahr, C;Swift, S;Begley, CG;Identification of a sensitive anti-erythropoietin receptor monoclonal antibody allows detection of low levels of EpoR in cellsJournal of immunological methods19887071Human EpoR expressing erythroid primary cells were derived from human mobilized peripheral blood CD34+ cells (AllCells, LLC, Emeryville, CA) by culture for 6 days in StemSpan SFEM media (STEMCELLS Tech; Vancouver, BC, Canada) with 10 ng/ml IL-3, 10 nFull Paper
2010Kim, ES;Kim, JJ;Park, EJ;Angiogenic factor-enriched platelet-rich plasma enhances in vivo bone formation around alloplastic graft materialThe journal of advanced prosthodontics21165181Commercially available human apheresed platelet plasmas were purchased from AllCells (Emeryville, CA, USA). Donors were restricted to males under 30 years-old for quality control. SaveFull Paper
2010Shytle, DR;Tan, J;Ehrhart, J;Smith, AJ;Sanberg, CD;Sanberg, PR;Anderson, J;Bickford, PC;Effects of blue-green algae extracts on the proliferation of human adult stem cells in vitro: a preliminary studyMedical science monitor : international medical journal of experimental and clinical research20037479For cell proliferation analysis, human bone marrow cells or human CD34+ cells (AllCells, Inc.) were cultured in 96 well plates (5×10 4 /well) containing 100 µL of complete medium (RPMI 1640 medium supplemented with 5% FCS). These cells were treated fFull Paper
2010Kawauchi, S;Terasaki, H;Katano, M;Murase, J;Masuda, Y;Tamura, T;Shimadzu, M;Quality control and monitoring for the isolation process of mesenchymal stem cells and their differentiation into osteoblastsGenetic testing and molecular biomarkers20187761Bone marrow samples (ABM001; AllCells, Emeryville, CA) were purchased after gaining approval of the ethical 270committee of the Olympus Corporation. Table 1 shows the ages and sexes of the donors. Skin-derived fibroblasts were also purchased from LonFull Paper
2010Abbott, C;Huang, G;Ellison, AR;Chen, C;Arora, T;Szilvassy, SJ;Wei, P;Mouse monoclonal antibodies against human c-Mpl and characterization for flow cytometry applicationsHybridoma (2005)20443702Primary human CD34 + cells isolated from normal bone marrow or G-CSF-mobilized peripheral blood were purchased from AllCells (Emeryville, CA). Cryopreserved cells were thawed SaveFull Paper
2010Clarkson-Jones, J;Page, C;Sarda, S;Swaisland, H;417 Human biotransformation of olaparib (AZD2281) an oral poly(ADP-ribose) polymerase (PARP) inhibitorEuropean Journal of Cancer SupplementsMethods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommended by the manufacturer (StemCell Technologies). Effects of SBFull Paper
2010Nokihara, H;Yamamoto, N;Yamada, Y;Asahina, H;Shibata, T;Seki, Y;Tamura, Y;Honda, K;Misawa, S;Tamura, T;415 A phase I study evaluating the safety profile and pharmacokinetics of CS-1008 (Tigatuzumab), humanized monoclonal antibody targeting death receptor 5 (DR5), in Japanese patients with advanced solid tumoursEuropean Journal of Cancer SupplementsMethods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommended by the manufacturer (StemCell Technologies). Effects of SBFull Paper
2010Padgett, C;Lim, J;Marschke, R;Northfelt, D;Andreopoulou, E;Hoff, D;Anderes, K;Ryckman, D;Chen, T;O'Brien, S;414 Clinical pharmacokinetics and pharmacodynamics of CX-4945, a novel inhibitor of protein kinase CK2: Interim report from the phase 1 clinical trialEuropean Journal of Cancer Supplementsthat is currently in phase Ib clinical trials in AML and MDS. Methods: Blasts from primary AML cells (obtained from AllCells; Emeryville, CA) were expanded using a StemSpan® medium and a cytokine cocktail (FLT3 ligand, SCF, IL-3 and IL-6) as recommenFull Paper
2010Antognoli, A;Rag2-/-; gammac-/-immunodeficient mice, a new preclinical model to study antitumor approachesThesisCD133+ human cord blood progenitor cells (derived from single donors) purchased from ALLCELLS (Emerville, CA), or kindly provided by Professor Lemoli of the Department of Hematology and Oncologic Sciences “L. e A. Seragnoli”, University of BolognaFull Paper
2010Tanavde, V;Commercialization of Cord BloodUmbilical Cord BloodAllCells, a California-based company and Lonza are leading players in this area. BioE also sells its MLPC cell line for research and is developing the use of MLPC as a platform for SaveFull Paper
2010Nowakiewicz, M;MST-based method for 6DOF rigid body motion planning in narrow passages2010 IEEE/RSJ International Conference on Intelligent Robots and Systemsconstruction of MST give priority to cells at levels equal or less than { by always treating their weights as lesser than weights of the other cells. 3) ALLCELLS() - Return the set of all leaf cells. 4) NEIGHBORHOOD(z) - Return the set of all cells iFull Paper
2010Briercheck, E;Freud, A;Caligiuri, M;Human natural killer cell developmentNatural Killer CellsFresh human BM was donated or purchased from AllCells, LLC (Berkeley, CA) and received within 24 hours of harvest. PB leukopaks were obtained from the American Red Cross. BM SaveFull Paper
2010Ringeisen, B;Othon, C;Wu, X;Krizman, D;Darfler, M;Anders, J;Wu, P;Biological Laser Printing (BioLP) for High Resolution Cell DepositionCell and Organ PrintingHUVEC were purchased from Allcells, LLC (Emeryville, California) and cultured according to the protocol provided. HUSMC were purchased from ATCC and cultured according to the protocol provided by the supplier. In both cases, per ml of media is fortifFull Paper
2010Leskov, I;Modeling hematologic malignancies and their treatment in humanized miceThesisPurified CD133* human umbilical cord hematopoietic stem cells (HSCs) were purchased from AllCells or from the National Disease Research Interchange (NDRI). Alternatively, CD133* cells were purified from fresh umbilical cord blood purchased from NDRI,Full Paper
2010Chao, MP;Modulating Phagocytosis: Therapeutic Targeting of the Anti-Phagocytic Signal CD47 in Human MalignanciesThesisHuman Samples Normal human bone marrow mononuclear cells were purchased from AllCells Inc. (Emeryville, CA, USA). Human AML samples (Figure 1B) were obtained from patients at the Stanford Medical Center with informed consent, according to an IRB-apprFull Paper
2010Lee, CH;Connective tissue growth factor (CCN2/CTGF)-induced fibrogenesis: Biology, bioengineering and therapeuticsThesisHuman mesenchymal stem cells (hMSCs) were isolated from commercially available fresh bone marrow samples of anonymous adult donors (AllCells, Berkeley, CA). Purification of hMSCs was accomplished by centrifugation through a density gradient (Ficoll-PFull Paper
2010Collins, JM;Stem cells are affected by physical and chemical components of the microenvironmental nicheThesisxperiments were performed on P2 hMSCs obtained from 2 separate donors. Additionally, some experiments were repeated with P2 hMSCs isolated from whole marrow (AllCells, Berkely, CA) as described previously (Alhadlaq et al., 2005)Full Paper
2010Scaife, MD;Truncated Cell Surface Markers Fused with Mutant Human Tmpk: Versatile Cell Fate Control Safety Cassettes for Lentiviral Vector Mediated Correction of Fabry DiseaseThesisHuman CD34+ cells were purchased from AllCells and cultured in Stem Span SFEM expansion medium (Stem Cell Technologies) and supplemented with 10 ng/ml human interleukin-6 (IL-6), 100 ng/ml human Flt3 Ligand (Flt3-L), 100 ng/ml human thrombopoietin (TFull Paper
2010Van Craenenbroeck, E;Exercise in chronic heart failure focus on endothelial repairThesistypesmost likely have important contributions to the initiation, continuation and regulation ofvascular homeostasis In the following, the term EPC is used for general description of allcells SaveFull Paper
2010Jangam, SR;A PCR based HIV-1 test for point-of-care applicationThesisIt was observed that there was a saturation effect being observed at total WBC 500,000 cells. To further confirm this observation, leukocyte depleted blood was spiked with different amounts of PBMC (ALLCELLS, Emeryville, CA) in the range of 46,000-2Full Paper
2010Sofia, MJ;Bao, D;Chang, W;Du, J;Nagarathnam, D;Rachakonda, S;Reddy, PG;Ross, BS;Wang, P;Zhang, HR;Bansal, S;Espiritu, C;Keilman, M;Lam, AM;Steuer, HM;Niu, C;Otto, MJ;Furman, PA;Discovery of a β-d-2'-deoxy-2'-α-fluoro-2'-β-C-methyluridine nucleotide prodrug (PSI-7977) for the treatment of hepatitis C virusJournal of medicinal chemistry20845908We acknowledge the following individuals and organizations for their contributions to the work described in this manuscript: Wuxi AppTec; ReachBio LLC; BASi; and Dr. Patrick Carroll, University of PennsylvaniaFull Paper
2010Lam, AM;Murakami, E;Espiritu, C;Steuer, HM;Niu, C;Keilman, M;Bao, H;Zennou, V;Bourne, N;Julander, JG;Morrey, JD;Smee, DF;Frick, DN;Heck, JA;Wang, P;Nagarathnam, D;Ross, BS;Sofia, MJ;Otto, MJ;Furman, PA;PSI-7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype inhibitor of hepatitis C virus replicationAntimicrobial agents and chemotherapy20516278Human bone marrow progenitor cell toxicity assays were performed by Emer Clarke at ReachBio LLC (Seattle, WA)Full Paper
2010Maloney, JM;Nikova, D;Lautenschläger, F;Clarke, E;Langer, R;Guck, J;Van Vliet, KJ;Mesenchymal stem cell mechanics from the attached to the suspended stateBiophysical journal20959088artment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts ¶Department of Physics, Cavendish Laboratory, University of Cambridge, Cambridge, United Kingdom ‖ReachBio, Seattle, Washington ∗Corresponding author kFull Paper
2009Nemunaitis, J;Clayman, G;Agarwala, SS;Hrushesky, W;Wells, JR;Moore, C;Hamm, J;Yoo, G;Baselga, J;Murphy, BA;Menander, KA;Licato, LL;Chada, S;Gibbons, RD;Olivier, M;Hainaut, P;Roth, JA;Sobol, RE;Goodwin, WJ;Biomarkers Predict p53 Gene Therapy Efficacy in Recurrent Squamous Cell Carcinoma of the Head and NeckClin. Cancer Res.19996201iplex primers (Affymetrix, Inc.). The resultant PCR products were analyzed using the Affymetrix p53 GeneChip, which encompasses all coding exons, 2 to 11, as well as immediate flanking regions. Confirmation of the results was carried out by sequencinFull Paper
2009Mori, F;Oda, N;Sakuragi, M;Sakakibara, F;Kiniwa, M;Miyoshi, K;New histopathological experimental model for benign prostatic hyperplasia: stromal hyperplasia in ratsJ. Urol.19095262All sections were developed by the avidin-biotin complex method. Sections were also stained with hematoxylin and eosin, and Masson's trichrome. Human BPH tissue samples (Folio Biosciences, Columbus, Ohio) were also stained. Histological AnalysisFull Paper
2009Fazzone, W;Wilson, PM;Labonte, MJ;Lenz, HJ;Ladner, RD;Histone deacetylase inhibitors suppress thymidylate synthase gene expression and synergize with the fluoropyrimidines in colon cancer cellsInt. J. Cancer19384949liquid nitrogen or fixed in 10% formalin. Formalin‐fixed tumor specimens were paraffin‐embedded and subsequently analyzed by immunohistochemistry (IHC) (QualTek Laboratories, Newtown, PA). IHC for TS was conducted asFull Paper
2009Donkor, J;Zhang, P;Wong, S;O'Loughlin, L;Dewald, J;Kok, BP;Brindley, DN;Reue, K;A conserved serine residue is required for the phosphatidate phosphatase activity but not the transcriptional coactivator functions of lipin-1 and lipin-2J. Biol. Chem.19717560Invitrogen). Plasmids were sequences to select templates for sense and antisense probe generation and linearized by digestion with BamHI. In situ hybridization was performed using the services of Phylogeny, Inc. (Columbus, OH)Full Paper
2009Maxwell, SA;Li, Z;Jaye, D;Jaya, D;Ballard, S;Ferrell, J;Fu, H;14-3-3zeta mediates resistance of diffuse large B cell lymphoma to an anthracycline-based chemotherapeutic regimenJ. Biol. Chem.195252243′. Preparation of DLBCL Tissue Extracts for Western Blotting. Normal and DLBCL lymph node tissues were procured through Folio Biosciences. Frozen tissue was ground to a powder in liquid nitrogen in a pestle and mortarFull Paper
2009Deroo, BJ;Rodriguez, KF;Couse, JF;Hamilton, KJ;Collins, JB;Grissom, SF;Korach, KS;Estrogen receptor beta is required for optimal cAMP production in mouse granulosa cellsMol. Endocrinol.19324971Mice were treated with vehicle or PMSG for 48 h, after which the ovaries were removed and snap frozen in 2-methylbutane (isopentane) and then stored at −70 C. In situ hybridization analysis for the detection of Lhcgr in whole ovaries was then carriedFull Paper
2009Cunningham, D;Spychala, K;McLarren, KW;Garza, LA;Boerkoel, CF;Herman, GE;Developmental expression pattern of the cholesterogenic enzyme NSDHL and negative selection of NSDHL-deficient cells in the heterozygous Bpa(1H)/+ mouseMol. Genet. Metab.19631568A darkfield view of a similar section hybridized with a control 35S-labeled sense Nsdhl RNA probe, showing little or no nonspecific background hybridization. (D) A brightfield view of a lateral sagittal section of the E15.5 embryo that includes the eFull Paper
2009Chen, LM;Verity, NJ;Chai, KX;Loss of prostasin (PRSS8) in human bladder transitional cell carcinoma cell lines is associated with epithelial-mesenchymal transition (EMT)BMC Cancer19849847(Hayward, CA). A Bladder Carcinoma TMA (ARY-HH0087) containing 80 tissue cores, each at 1.5 mm in diameter; with 40 cancer tissues and 40 matching or independent non-cancerous tissues was obtained from Folio Biosciences (Columbus, OH)Full Paper
2009Binkert, CA;Drooz, AT;Caridi, JG;Sands, MJ;Bjarnason, H;Lynch, FC;Rilling, WS;Zambuto, DA;Stavropoulos, SW;Venbrux, AC;Kaufman, JA;Technical success and safety of retrieval of the G2 filter in a prospective, multicenter studyJ Vasc Interv Radiol19875062Full Paper
2009Lynch, FC;Balloon-assisted removal of tilted inferior vena cava filters with embedded tipsJ Vasc Interv Radiol19729132Full Paper
2009Lynch, FC;Kekulawela, S;Removal of the G2 filter: differences between implantation times greater and less than 180 daysJ Vasc Interv Radiol19640738Full Paper
2009Cantwell, CP;Pennypacker, J;Singh, H;Scorza, LB;Waybill, PN;Lynch, FC;Comparison of the recovery and G2 filter as retrievable inferior vena cava filtersJ Vasc Interv Radiol19640733Full Paper
2009Choi, KD;Vodyanik, MA;Slukvin, II;Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitorsThe Journal of clinical investigation19726877CD1a+ DCs, or LCs were cocultured with 1 × 105 adult peripheral (AllCells) or cord blood (Cambrex) mononuclear cells in RPMI 1640 medium (Invitrogen) containing 5% human AB serum (Sigma-Aldrich) in 96-well flat-bottom plates. At 6 days of culture, thFull Paper
2009Pfeffer, BA;Bernstein, SA;Bartels, SP;Preservation of structure and immunoreactivity at the vitreoretinal interface of the rabbit eyeGraefes Arch. Clin. Exp. Ophthalmol.19020891Presented in part at the 2006 meeting of The Association for Research in Vision and Ophthalmology, Fort Lauderdale, Florida. This study was supported by Bausch & Lomb, Inc., with whom QualTek Molecular Laboratories has a contractual relationshipFull Paper
2009Petrie, KR;Schenk, T;Chornet, MB;Howell, L;Dick, JE;Modulation of Histone H3K4 and H3K27 Methylation Levels Via Pharmacological Inhibition of LSD1 and Degradation of the EZH2-Containing Polycomb Repressive Complex 2 Stimulates ATRA-Mediated Differentiation of AML CellsConferenceFlow cytometric detection of apoptosis among normal mononuclear cells obtained from the bone marrow of healthy volunteers (AllCells, LLC) by phycoerythrin (PE)conjugated Annexin- SaveFull Paper
2009Muchamuel, T;Basler, M;Aujay, MA;Suzuki, E;Kalim, KW;Lauer, C;Sylvain, C;Ring, ER;Shields, J;Jiang, J;Shwonek, P;Parlati, F;Demo, SD;Bennett, MK;Kirk, CJ;Groettrup, M;A selective inhibitor of the immunoproteasome subunit LMP7 blocks cytokine production and attenuates progression of experimental arthritisNature medicine19525961We purchased human PBMCs from normal healthy volunteers from AllCells, and we obtained PBMCs from individuals with rheumatoid arthritis (who had given informed consent) from a SaveFull Paper
2009Majeti, R;Chao, MP;Alizadeh, AA;Pang, WW;Jaiswal, S;Gibbs, KD;van Rooijen, N;Weissman, IL;CD47 is an adverse prognostic factor and therapeutic antibody target on human acute myeloid leukemia stem cellsCell19632179Acute myeloid leukemia (AML) is organized as a cellular hierarchy initiated and maintained by a subset of self-renewing leukemia stem cells (LSC). We hypothesized that increased SaveFull Paper
2009Schmitt, N;Morita, R;Bourdery, L;Bentebibel, SE;Zurawski, SM;Banchereau, J;Ueno, H;Human dendritic cells induce the differentiation of interleukin-21-producing T follicular helper-like cells through interleukin-12Immunity19592276sorting with FACSAria (BD) as CCR7+ CD8- CD56- HLA-DR- CD45RA+ CD4+ cells. Memory CD4+ T cells were directly sorted from PBMCs as CD8- CD56- CD45RA- CD4+ cells. Cord blood naïve CD4 T cells were sorted from frozen cells (AllCells) as CCR7+ CD8- CD56-Full Paper
2009Wang, Y;Sul, HS;Pref-1 regulates mesenchymal cell commitment and differentiation through Sox9Cell metabolism19254573Human-derived marrow stromal cells (MSC) and MSC media were purchased from Allcells. Sox9 target-specific siRNA (sc-36534) (Santa Cruz) and the control siRNA (sc-36869) were SaveFull Paper
2009Trifari, S;Kaplan, CD;Tran, EH;Crellin, NK;Spits, H;Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cellsNature immunology19578368Naive CD4 + T cells were obtained from human cord blood (AllCells) with a Naive CD4 + T cell Isolation Kit II (Miltenyi), followed by sorting of the CD4 + CD45RA + CD45RO − SaveFull Paper
2009Ye, Z;Zhan, H;Mali, P;Dowey, S;Williams, DM;Jang, YY;Dang, CV;Spivak, JL;Moliterno, AR;Cheng, L;Human-induced pluripotent stem cells from blood cells of healthy donors and patients with acquired blood disordersBlood19797525Isolated PB CD34 + cells after G-CSF mobilization were purchased from AllCells and used as a normal control for analyzing gene expression. Four classic retroviral vectors pMXs-Oct4, SaveFull Paper
2009Parlati, F;Lee, SJ;Aujay, M;Suzuki, E;Levitsky, K;Lorens, JB;Micklem, DR;Ruurs, P;Sylvain, C;Lu, Y;Shenk, KD;Bennett, MK;Carfilzomib can induce tumor cell death through selective inhibition of the chymotrypsin-like activity of the proteasomeBlood19671918Rosen's laboratory at Northwestern University. CD138+ cells and peripheral blood mononuclear cells (PBMCs) were purchased from Allcells. The purity of CD138+ cells was greater than 90% as determined by the supplier. HS-Sultan-GFPu cell lines were genFull Paper
2009Figueroa, ME;Skrabanek, L;Li, Y;Jiemjit, A;Fandy, TE;Paietta, E;Fernandez, H;Tallman, MS;Greally, JM;Carraway, H;Licht, JD;Gore, SD;Melnick, A;MDS and secondary AML display unique patterns and abundance of aberrant DNA methylationBlood19652201Support Laboratory, Cincinnati Children's Hospital, and 4 from Allcells. Matched CD34+ and CD34− fractions were obtained from an additional 4 healthy donors through the Stem Cell and Xenograft Core, University of Pennsylvania School of MedicineFull Paper
2009Hasstedt, SJ;Bezemer, ID;Callas, PW;Vossen, CY;Trotman, W;Hebbel, RP;Demers, C;Rosendaal, FR;Bovill, EG;Cell adhesion molecule 1: a novel risk factor for venous thrombosisBlood19643986cover slips coated with gelatin (Allcells Inc) under sterile conditions at 37C, 5% CO 2 in HUVEC Basal Medium containing HUVEC Basal Medium Supplement (Allcells Inc). Cells were SaveFull Paper
2009Shi, P;Lai, R;Lin, Q;Iqbal, AS;Young, LC;Kwak, LW;Ford, RJ;Amin, HM;IGF-IR tyrosine kinase interacts with NPM-ALK oncogene to induce survival of T-cell ALK+ anaplastic large-cell lymphoma cellsBlood19423729Type I insulin-like growth factor receptor (IGF-IR) tyrosine kinase plays important roles in the pathogenesis of several malignancies. Although it promotes the growth of stimulated SaveFull Paper
2009Loh, YH;Agarwal, S;Park, IH;Urbach, A;Huo, H;Heffner, GC;Kim, K;Miller, JD;Ng, K;Daley, GQ;Generation of induced pluripotent stem cells from human bloodBlood19299331obtained from Allcells (Emeryville, CA) were collected from a 26-year-old male donor, and CD34+ cells (mPB014F) were isolated. Generation of CD34 iPS cells was performed using a protocol modified from previously published methods.9Full Paper
2009Nahimana, A;Attinger, A;Aubry, D;Greaney, P;Ireson, C;Thougaard, AV;Tjørnelund, J;Dawson, KM;Dupuis, M;Duchosal, MA;The NAD biosynthesis inhibitor APO866 has potent antitumor activity against hematologic malignanciesBlood19196867APO866 inhibits nicotinamide phosphoribosyltransferase (NMPRTase), a key enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis from the natural precursor SaveFull Paper
2009Nardi, V;Naveiras, O;Azam, M;Daley, GQ;ICSBP-mediated immune protection against BCR-ABL-induced leukemia requires the CCL6 and CCL9 chemokinesBlood19171873eripheral blood mononuclear cells from healthy volunteers were purchased from AllCells (Emeryville, CA) and maintained in complete RPMI medium. Murine and human cells were treated with IFN alpha 2A or IFN beta (R&D SystemsFull Paper
2009Figueroa, ME;Wouters, BJ;Skrabanek, L;Glass, J;Li, Y;Erpelinck-Verschueren, CA;Langerak, AW;Löwenberg, B;Fazzari, M;Greally, JM;Valk, PJ;Melnick, A;Delwel, R;Genome-wide epigenetic analysis delineates a biologically distinct immature acute leukemia with myeloid/T-lymphoid featuresBlood191687924 acquired from the Translational Trials Development and Support Laboratory, Cincinnati Children's Hospital (Cincinnati, OH) and 4 purchased from Allcells (Emeryville, CA). This research was approved by the institutional review boards at Albert EinstFull Paper
2009Chen, H;Campbell, RA;Chang, Y;Li, M;Wang, CS;Li, J;Sanchez, E;Share, M;Steinberg, J;Berenson, A;Shalitin, D;Zeng, Z;Gui, D;Perez-Pinera, P;Berenson, RJ;Said, J;Bonavida, B;Deuel, TF;Berenson, JR;Pleiotrophin produced by multiple myeloma induces transdifferentiation of monocytes into vascular endothelial cells: a novel mechanism of tumor-induced vasculogenesisBlood19060246The human RPMI8226 and U266 MM cell lines and monocyte (THP-1) and myelomonocytic (U937) cell lines were obtained from ATCC (Manassas, VA). Normal human BM was purchased from Allcells (Berkeley, CA). Highly purified human T cells from healthy donorsFull Paper
2009Luo, XM;Maarschalk, E;O'Connell, RM;Wang, P;Yang, L;Baltimore, D;Engineering human hematopoietic stem/progenitor cells to produce a broadly neutralizing anti-HIV antibody after in vitro maturation to human B lymphocytesBlood1905987690 minutes at 50 000 × g. Human cord blood CD34+ HSPCs were purchased from AllCells (Emeryville, CA) and maintained for less than 24 hours before infection in Iscove modified Dulbecco medium containing 10% fetal bovine serum and cytokines. The cytokiFull Paper
2009Joshi, I;Minter, LM;Telfer, J;Demarest, RM;Capobianco, AJ;Aster, JC;Sicinski, P;Fauq, A;Golde, TE;Osborne, BA;Notch signaling mediates G1/S cell-cycle progression in T cells via cyclin D3 and its dependent kinasesBlood19001083Notch signaling is critical for the regulation of differentiation, proliferation and apoptosis in many cell types. Notch receptors and various Notch ligands have been shown to have a SaveFull Paper
2009Bedi, R;Du, J;Sharma, AK;Gomes, I;Ackerman, SJ;Human C/EBP-epsilon activator and repressor isoforms differentially reprogram myeloid lineage commitment and differentiationBlood18832658Cord blood (CB) CD34+ cells ( 97% pure) purchased from AllCells (Emeryville, CA) were prestimulated for 18 hours at 106 cells/mL in presence of 100 ng/mL recombinant human stem cell factor (rh-SCF), recombinant human fms-like tyrosine kinase 3 ligandFull Paper
2009Oda, M;Glass, JL;Thompson, RF;Mo, Y;Olivier, EN;Figueroa, ME;Selzer, RR;Richmond, TA;Zhang, X;Dannenberg, L;Green, RD;Melnick, A;Hatchwell, E;Bouhassira, EE;Verma, A;Suzuki, M;Greally, JM;High-resolution genome-wide cytosine methylation profiling with simultaneous copy number analysis and optimization for limited cell numbersNucleic acids research19386619One million fresh CD34 + bone marrow progenitors were purchased from Allcells (Emerville, CA). DNA was extracted from these cells and checked for purity and amount by SaveFull Paper
2009O'Brien, JJ;Baglole, CJ;Garcia-Bates, TM;Blumberg, N;Francis, CW;Phipps, RP;15-deoxy-Delta12,14 prostaglandin J2-induced heme oxygenase-1 in megakaryocytes regulates thrombopoiesisJournal of thrombosis and haemostasis : JTH18983509Human CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5×105 cells per well in a 12-well plate and cultured in serum-free medium as previously described (19) and supplemented with 100 ng/mL of recombinant huFull Paper
2009Liu, PC;Caulder, E;Li, J;Waeltz, P;Margulis, A;Wynn, R;Becker-Pasha, M;Li, Y;Crowgey, E;Hollis, G;Haley, P;Sparks, RB;Combs, AP;Rodgers, JD;Burn, TC;Vaddi, K;Fridman, JS;Combined inhibition of Janus kinase 1/2 for the treatment of JAK2V617F-driven neoplasms: selective effects on mutant cells and improvements in measures of disease severityClinical cancer research : an official journal of the American Association for Cancer Research19887489Mononuclear cells were isolated from peripheral blood samples from patients with PV or normal control individuals by centrifugation through Ficoll (Allcells, Inc.). Colony forming assays SaveFull Paper
2009Cardarelli, PM;Moldovan-Loomis, MC;Preston, B;Black, A;Passmore, D;Chen, TH;Chen, S;Liu, J;Kuhne, MR;Srinivasan, M;Assad, A;Witte, A;Graziano, RF;King, DJ;In vitro and in vivo characterization of MDX-1401 for therapy of malignant lymphomaClinical cancer research : an official journal of the American Association for Cancer Research19401346ADCC assay. Human peripheral blood mononuclear effector cells were purified from either heparinized whole blood (AllCells) or buffy coat (Stanford Blood Center) by standard Ficoll-Paque separation. Cells (2 × 106) were washed in PBS and sent for genoFull Paper
2009Zhong, C;Qu, X;Tan, M;Meng, YG;Ferrara, N;Characterization and regulation of bv8 in human blood cellsClinical cancer research : an official journal of the American Association for Cancer Research19336519Isolation of whole human bone marrow cells from fresh bone marrow collections. Fresh bone marrow samples were obtained from ALLCELLS. The bone marrow was first diluted with 10% fetal bovine serum-containing DMEM and then passed through a 40-μm cell sFull Paper
2009Ara, T;Song, L;Shimada, H;Keshelava, N;Russell, HV;Metelitsa, LS;Groshen, SG;Seeger, RC;DeClerck, YA;Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cellsCancer research19118018Human BMSC were either purchased from ALLCells LLC or obtained from bone marrow samples of patients with neuroblastoma enrolled by the Children's Oncology Group. These SaveFull Paper
2009Senyuk, V;Rinaldi, CR;Li, D;Cattaneo, F;Stojanovic, A;Pane, F;Du, X;Mahmud, N;Dickstein, J;Nucifora, G;Consistent up-regulation of Stat3 Independently of Jak2 mutations in a new murine model of essential thrombocythemiaCancer research19118011Mononuclear cells were separated from the BM aspirates by Ficoll-Hypaque centrifugation. The samples were obtained according to guidelines established by the University of Illinois at Chicago Institutional Review Board. Healthy donors’ BM cells wereFull Paper
2009Kim, YC;Wu, Q;Chen, J;Xuan, Z;Jung, YC;Zhang, MQ;Rowley, JD;Wang, SM;The transcriptome of human CD34+ hematopoietic stem-progenitor cellsProceedings of the National Academy of Sciences of the United States of America19416867De Novo Collection of CD34+ 3′ ESTs. Bone marrow CD34+ cells of 3 healthy donors were purchased from AllCells. 3′ ESTs were collected by using the GLGI method (18, 19). A total of 10,000 CD34+ SAGE tags identified in a previous study (12) were selectFull Paper
2009Majeti, R;Becker, MW;Tian, Q;Lee, TL;Yan, X;Liu, R;Chiang, JH;Hood, L;Clarke, MF;Weissman, IL;Dysregulated gene expression networks in human acute myelogenous leukemia stem cellsProceedings of the National Academy of Sciences of the United States of America19218430Normal human bone marrow mononuclear cells were either purchased from AllCells or obtained from National Marrow Donor Program discarded filter units. For AML specimens, peripheral blood and/or bone marrow was obtained following informed consent at thFull Paper
2009Bernard, JJ;Seweryniak, KE;Koniski, AD;Spinelli, SL;Blumberg, N;Francis, CW;Taubman, MB;Palis, J;Phipps, RP;Foxp3 regulates megakaryopoiesis and platelet functionArteriosclerosis, thrombosis, and vascular biology19661482Human CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5×105 cells per well in a 12-well plate and cultured in serum-free medium as previously described 12 and supplemented with 100 ng/mL of recombinant humaFull Paper
2009Jekle, A;Chow, E;Kopetzki, E;Ji, C;Yan, MJ;Nguyen, R;Sankuratri, S;Cammack, N;Heilek, G;CD4-BFFI: a novel, bifunctional HIV-1 entry inhibitor with high and broad antiviral potencyAntiviral research19559732obtained from ATCC, Manassas, VA. Human PBMC were obtained from AllCells (Emeryville, CA), stimulated for one day in PBMC media (RPMI-1640 media containing 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 2 mM l-glutamine, 1 mM sodium-pyruvaFull Paper
2009Nakahara, K;Wakasa-Morimoto, C;Kobayashi, M;Miki, S;Noshi, T;Seki, T;Kanamori-Koyama, M;Kawauchi, S;Suyama, A;Fujishita, T;Yoshinaga, T;Garvey, EP;Johns, BA;Foster, SA;Underwood, MR;Sato, A;Fujiwara, T;Secondary mutations in viruses resistant to HIV-1 integrase inhibitors that restore viral infectivity and replication kineticsAntiviral research19027039Passage of HIV-1 in the presence of integrase inhibitors (INIs) generates resistant viruses that have mutations in the integrase region. Integrase-resistant mutations Q148K and Q148R SaveFull Paper
2009Strickland, LA;Ross, J;Williams, S;Ross, S;Romero, M;Spencer, S;Erickson, R;Sutcliffe, J;Verbeke, C;Polakis, P;van Bruggen, N;Koeppen, H;Preclinical evaluation of carcinoembryonic cell adhesion molecule (CEACAM) 6 as potential therapy target for pancreatic adenocarcinomaThe Journal of pathology19334050consenting donors; bone marrow samples and purified CD34+ cells (AllCells Inc., Berkeley, CA, USA) were received within 5 (unfractionated samples) or 24 (fractionated samples) h of collection. PC3 (CRL-1435) and HPAFII (CRL-2119) cells were obtainedFull Paper
2009Leleu, X;Hunter, ZR;Xu, L;Roccaro, AM;Moreau, AS;Santos, DD;Hatjiharissi, E;Bakthavachalam, V;Adamia, S;Ho, AW;Soumerai, J;Patterson, CJ;Manning, RJ;Hamilton, S;Verselis, S;Fox, E;Carrasco, R;Ghobrial, IM;Treon, SP;Expression of regulatory genes for lymphoplasmacytic cell differentiation in Waldenstrom MacroglobulinemiaBritish journal of haematology19220283years and the male/female ratio was 1·6. All WM patients were previously untreated. Table I summarizes the characteristics of the patients. Bone marrow aspirates from healthy donors were used in comparison (AllCells LLC, Berkeley, CA, USA)Full Paper
2009Krupa, A;Fudala, R;Stankowska, D;Loyd, T;Allen, TC;Matthay, MA;Gryczynski, Z;Gryczynski, I;Mettikolla, YV;Kurdowska, AK;Anti-chemokine autoantibody:chemokine immune complexes activate endothelial cells via IgG receptorsAmerican journal of respiratory cell and molecular biology19109244HUVECs were purchased from Cambrex (Walkersville, MD) or Allcells (Berkeley, CA), and cultured in endothelial basal medium containing endothelial growth factor, hydrocortisone, gentamicin, amphoreticin B, bovine brain extract, heparin, and FBS (EGM-MFull Paper
2009Lee, JH;Ort, T;Ma, K;Picha, K;Carton, J;Marsters, PA;Lohmander, LS;Baribaud, F;Song, XY;Blake, S;Resistin is elevated following traumatic joint injury and causes matrix degradation and release of inflammatory cytokines from articular cartilage in vitroOsteoarthritis and cartilage19095472Human primary monocytes isolated from three normal healthy volunteers were purchased from AllCells, LLC (Emeryville, CA). The cells were treated with recombinant human resistin (Peprotech, Inc, Rocky Hill, NJ; endotoxin level was measured as 0.9 EU/mFull Paper
2009Hashimoto, S;Nishiyama, T;Hayashi, S;Takebe, K;Kanzaki, N;Fujishiro, T;Kuroda, R;Kurosaka, M;224 THE FUNCTION OF p53 ON HUMAN CHONDROCYTE APOPTOSIS IN RESPONSE TO SHEAR STRESSOsteoarthritis and Cartilageated by enzymatic digestion of bone from patients undergoing knee endoprothesis due to arthrosis of the knee joint. Human macrophages were differentiated from CD14+ peripheral blood monocytes (Allcells) by treatment with macrophage colonyFull Paper
2009Peterson, SJ;Kim, DH;Li, M;Positano, V;Vanella, L;Rodella, LF;Piccolomini, F;Puri, N;Gastaldelli, A;Kusmic, C;L'Abbate, A;Abraham, NG;The L-4F mimetic peptide prevents insulin resistance through increased levels of HO-1, pAMPK, and pAKT in obese miceJournal of lipid research19224872at various time points (0-120 min) for measurement of blood glucose levels. Human bone marrow-derived mesenchymal stem cells and adipocytes Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing the cells,Full Paper
2009Patel, E;Wang, B;Lien, L;Wang, Y;Yang, LJ;Moreb, JS;Chang, LJ;Diverse T-cell differentiation potentials of human fetal thymus, fetal liver, cord blood and adult bone marrow CD34 cells on lentiviral Delta-like-1-modified mouse stromal cellsImmunology19740310Invitrogen, Carlsbad, CA) supplemented with 20% fetal bovine serum and 1% penicillin-streptomycin. The adult BM and CB CD34+ cells were purchased from AllCells, LLC (Emeryville, CA), and Cambrex Corp. (Baltimore, MD) Aborted fetal liver and thymus tiFull Paper
2009Sutherland, MK;Yu, C;Lewis, TS;Miyamoto, JB;Morris-Tilden, CA;Jonas, M;Sutherland, J;Nesterova, A;Gerber, HP;Sievers, EL;Grewal, IS;Law, CL;Anti-leukemic activity of lintuzumab (SGN-33) in preclinical models of acute myeloid leukemiamAbs20065652Primary normal human monocytes and primary AML patient bone marrow samples were obtained from AllCells (Emeryville, CA). Fluorochrome-conjugated antibodies against human SaveFull Paper
2009Nan, JL;Li, JJ;He, JG;C-reactive protein decreases interleukin-8 production in human endothelial progenitor cells by inhibition of p38 MAPK pathwayChinese medical journal19781372Theabilityto modulate the antigenic pbenotype in response to the environmentiscommon to allcells.ECsmay regulate 1evelsofvascularendothelialgrowthfactorreceptor一2 in response to SaveFull Paper
2009Patterson, ST;Li, J;Kang, JA;Wickrema, A;Williams, DB;Reithmeier, RA;Loss of specific chaperones involved in membrane glycoprotein biosynthesis during the maturation of human erythroid progenitor cellsThe Journal of biological chemistry19258325hematopoietic progenitors were either purchased from Stem Cell Technologies (mPB015F) or initially isolated from growth factor-mobilized peripheral blood (purchased from AllCells, SaveFull Paper
2009Ji, C;Kopetzki, E;Jekle, A;Stubenrauch, KG;Liu, X;Zhang, J;Rao, E;Schlothauer, T;Fischer, S;Cammack, N;Heilek, G;Ries, S;Sankuratri, S;CD4-anchoring HIV-1 fusion inhibitor with enhanced potency and in vivo stabilityThe Journal of biological chemistry19097993In this study, we describe a novel CD4-targeting bifunctional human immunodeficiency virus (HIV-1) fusion inhibitor (CD4-BFFI) that blocks HIV-1 entry by inhibiting both HIV-1 SaveFull Paper
2009Hirschler-Laszkiewicz, I;Tong, Q;Conrad, K;Zhang, W;Flint, WW;Barber, AJ;Barber, DL;Cheung, JY;Miller, BA;TRPC3 activation by erythropoietin is modulated by TRPC6The Journal of biological chemistry19074769Human erythroid progenitors were also cultured using a two-phase liquid culture system (44, 45). Cells were harvested at day 8 of phase II and were predominantly proerythroblasts or basophilic normoblasts. CD34+ cells were purchased from AllCells, LLFull Paper
2009Zhang, Q;Shi, S;Liu, Y;Uyanne, J;Shi, Y;Shi, S;Le, AD;Mesenchymal stem cells derived from human gingiva are capable of immunomodulatory functions and ameliorate inflammation-related tissue destruction in experimental colitisJournal of immunology (Baltimore, Md. : 1950)19923445Human bone marrow aspirates from healthy adult donors (20-35 years of age) were purchased from AllCells and cultured with α-MEM supplemented with 10% FBS, 100 μM l-ascorbic acid-2-phosphate, 2 mM l-glutamine, 100 U/ml penicillin, and 100 μg/ml streptFull Paper
2009Parrish, YK;Baez, I;Milford, TA;Benitez, A;Galloway, N;Rogerio, JW;Sahakian, E;Kagoda, M;Huang, G;Hao, QL;Sevilla, Y;Barsky, LW;Zielinska, E;Price, MA;Wall, NR;Dovat, S;Payne, KJ;IL-7 Dependence in human B lymphopoiesis increases during progression of ontogeny from cord blood to bone marrowJournal of immunology (Baltimore, Md. : 1950)19299724CB was collected according to guidelines approved by the Institutional Review Boards at Childrens Hospital Los Angeles and Loma Linda University. Adult human BM was purchased from AllCells, LLC, Berkeley, CA; the National Disease Research InterchangeFull Paper
2009Vendel, AC;Calemine-Fenaux, J;Izrael-Tomasevic, A;Chauhan, V;Arnott, D;Eaton, DL;B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNKJournal of immunology (Baltimore, Md. : 1950)19155498Tonsil tissue (AllCells) was dissociated and a single-cell suspension was stained with anti-CD19, anti-IgD, and anti-CD38. Naive tonsillar B cells were identified as CD19 + IgD + CD38 SaveFull Paper
2009Panetta, NJ;Gupta, DM;Kwan, MD;Wan, DC;Commons, GW;Longaker, MT;Tissue harvest by means of suction-assisted or third-generation ultrasound-assisted lipoaspiration has no effect on osteogenic potential of human adipose-derived stromal cellsPlastic and reconstructive surgery19568046Commercially available bone marrow mesenchymal stem cells (AllCells), subjected to similar experimental osteogenic differentiation conditions, demonstrated significantly less staining SaveFull Paper
2009Weigel-Van Aken, KA;Pharmacological activation of guanine nucleotide exchange factors for the small GTPase Rap1 recruits high-affinity beta1 integrins as coreceptors for parvovirus B19: improved ex vivo gene transfer to human erythroid progenitor cellsHuman gene therapy19702438Human bone marrow-derived CD36 + erythroid progenitor cells were purchased from AllCells (Emeryville, CA). Cells were cultured overnight in serum-free medium containing stem cell SaveFull Paper
2009Zinckgraf, JW;Sposato, M;Zielinski, V;Powell, D;Treanor, JJ;von Hofe, E;Identification of HLA class II H5N1 hemagglutinin epitopes following subvirion influenza A (H5N1) vaccinationVaccine19596415Since we did not have access to pre-vaccine samples, eight random naive donor PBMC samples (AllCells, Emeryville, CA) were utilized to assess potential cross-reactive T cell SaveFull Paper
2009Morizono, K;Pariente, N;Xie, Y;Chen, IS;Redirecting lentiviral vectors by insertion of integrin-tageting peptides into envelope proteinsThe journal of gene medicine19434609Human umbilical vein endothelial cells (HUVEC) were purchased from AllCells (Emeryville, CA, USA) and Lonza (Walkersville, MD, USA). HUVEC were cultured in EBM-2 (Lonza) supplemented with EGM-2 singlequots (Lonza). When HUVEC started growing slowly,Full Paper
2009Liang, M;Pariente, N;Morizono, K;Chen, IS;Targeted transduction of CD34+ hematopoietic progenitor cells in nonpurified human mobilized peripheral blood mononuclear cellsThe journal of gene medicine19152374Cells binding with the anti-CD34 antibody were separated by a MACS machine (Miltenyi Biotec). Human primary PBMCs were isolated from leukopacks, Ficoll purified and cultured in RPMI with 20% FCS (Omega Scientific, Tarzana, CA, USA). Frozen human mobiFull Paper
2009Lee, CH;Marion, NW;Hollister, S;Mao, JJ;Tissue formation and vascularization in anatomically shaped human joint condyle ectopically in vivoTissue engineering. Part A19563263hMSCs were isolated from fresh bone marrow samples of multiple anonymous adult donors (AllCells, Berkeley, CA) per our prior methods. Purification of hMSCs was accomplished by SaveFull Paper
2009李文芳, ;黄元庆, ;基于虚拟仪器和 DDE 发电机组温度实时数据采集系统的研制厦门大学学报 (自然科学版)其对应的 DDE 服务名,主题名和项名应用与服务器端一致, 分 别是"wd" ,"DDE wd Test"和"AllCells" .在LabWindows/CVI 实时数据库中, 操作的对象是点AllCells , 是一组油温参数的集合, 可以 SaveFull Paper
2009Hall, AA;Leonardo, CC;Collier, LA;Rowe, DD;Willing, AE;Pennypacker, KR;Delayed treatments for stroke influence neuronal death in rat organotypic slice cultures subjected to oxygen glucose deprivationNeuroscience19723565HUCBC (AllCells, LLC, Inc.) were thawed and resuspended in DMEM containing DNase (1ug/ml) to prevent aggregation. HUCBC were washed twice and resuspended at a concentration of 108 cells/ml. Cultures were maintained in a standard tissue culture incubaFull Paper
2009Fukushima-Shintani, M;Suzuki, K;Iwatsuki, Y;Abe, M;Sugasawa, K;Hirayama, F;Kawasaki, T;Nakahata, T;AKR-501 (YM477) a novel orally-active thrombopoietin receptor agonistEuropean journal of haematology19183407Human cord blood (CB) CD34+ cells (AllCells, Berkeley, CA, USA) at a density of 2500 cells/0.75 mL were suspended in MegaCult™-C (StemCell Technologies Inc., Vancouver, Canada) containing either AKR-501 dissolved in DMSO or rhTPO, and plated in two-wFull Paper
2009Zhao, Z;He, X;Bi, Y;Xia, Y;Tao, N;Li, L;Ma, Q;Induction of CYP4F3 by benzene metabolites in human white blood cells in vivo in human promyelocytic leukemic cell lines and ex vivo in human blood neutrophilsDrug metabolism and disposition: the biological fate of chemicals19029204Whole blood from a healthy male adult was purchased from AllCells, LLC (Emeryville, CA). Neutrophils were isolated according a protocol provided by AllCells, LLC. In brief, whole SaveFull Paper
2009Nishino, T;Miyaji, K;Ishiwata, N;Arai, K;Yui, M;Asai, Y;Nakauchi, H;Iwama, A;Ex vivo expansion of human hematopoietic stem cells by a small-molecule agonist of c-MPLExperimental hematology197445390.5 U/mL rhEPO, and 10 ng/mL rhTPO, respectively. Human cord blood (hCB) CD34+ cells were purchased from Lonza (Basel, Switzerland) and AllCells (Berkeley, CA, USA) or purified from hCB. Fresh hCB cells were obtained from the Tokyo Cord Blood Bank (TFull Paper
2009Giammona, LM;Panuganti, S;Kemper, JM;Apostolidis, PA;Lindsey, S;Papoutsakis, ET;Miller, WM;Mechanistic studies on the effects of nicotinamide on megakaryocytic polyploidization and the roles of NAD+ levels and SIRT inhibitionExperimental hematology19715739Cultures were initiated in T-flasks with previously frozen mPB CD34-selected cells (AllCells; Berkeley, CA or Fred Hutchinson Cancer Research Center; Seattle, WA) and maintained at a concentration of 100,000 to 300,000 cells/ml as described [8]. MostFull Paper
2009Gibson, JD;Jakuba, CM;Boucher, N;Holbrook, KA;Carter, MG;Nelson, CE;Single-cell transcript analysis of human embryonic stem cellsIntegrative biology : quantitative biosciences from nano to macro20023769We demonstrate the qualitative and quantitative power of single-cell transcript analysis to characterize transcriptome dynamics in human embryonic stem cells (hESC's). Single-cell SaveFull Paper
2009Wells, R;Guth, A;Lappin, M;Dow, S;Anti-endothelial cell antibodies in dogs with immune-mediated hemolytic anemia and other diseases associated with high risk of thromboembolismJournal of veterinary internal medicine19192144AllCells LLC, Emeryville, CA bSigma Aldrich, St Louis, MO cGibco-InVitrogen, San Diego, CA dHyclone, Logan, UT eJackson ImmunoResearch, West Grove, PA fDako-Cytomation, Fort Collins, COFull Paper
2009Harriman, WD;Collarini, EJ;Cromer, RG;Dutta, A;Strandh, M;Zhang, F;Kauvar, LM;Multiplexed Elispot assayJournal of immunological methods19084532Normal human PBMCs were obtained from AllCells (Emeryville, CA). For CellSpot assays, 105 PBMC, Th1 or Th2 selected cells were suspended in activation medium with phorbol myristate acetate (PMA) at 50 ng/mL and Ionomycin at 1 µg/mL or control medium,Full Paper
2009Sugiyama, E;Lee, SJ;Lee, SS;Kim, WY;Kim, SR;Tohkin, M;Hasegawa, R;Okuda, H;Kawamoto, M;Kamatani, N;Sawada, J;Kaniwa, N;Saito, Y;Shin, JG;Ethnic differences of two non-synonymous single nucleotide polymorphisms in CDA geneDrug metabolism and pharmacokinetics20045991DNA from 200 healthy Chinese-Americans was extracted from cord blood samples purchased from AllCells (Emeryville, CA, USA). Peripheral blood samples from healthy Caucasian- SaveFull Paper
2009Ye, D;Garcia-Manero, G;Kantarjian, HM;Xiao, L;Vadhan-Raj, S;Fernandez, MH;Nguyen, MH;Medeiros, LJ;Bueso-Ramos, CE;Analysis of Aurora kinase A expression in CD34(+) blast cells isolated from patients with myelodysplastic syndromes and acute myeloid leukemiaJournal of hematopathology19669217reverse transcription efficiency. Relative RNA level was reported via standard delta delta Ct (δδ Ct). cDNA purified from normal CD34+ hematopoietic progenitor cells was used as calibrator sample (Allcells, LLC, Emeryville, CA, USA). ImmunocytochemiFull Paper
2009Nagel, I;Identifizierung von pathogenetisch relevanten Genen bei lymphatischen Neoplasien unter besonderer Berücksichtigung des langen Arms von Chromosom 14ThesisProbanden isoliert. Zur Normalisierung der quantitativen Reverse Transkription (RT)-PCR-Analyse des TRAF3-Gens wurde „Normal Peripheral Blood B Cells cDNA (CD19+)“ der Firma Allcells (Emeryville, CA, USA) verwendet. Für die quantitativen RT-PCR-AnalyFull Paper
2009Mahadevan, D;Choi, J;Cooke, L;Simons, B;Riley, C;Klinkhammer, T;Sud, R;Maddipoti, S;Hehn, S;Garewal, H;Spier, C;Gene Expression and Serum Cytokine Profiling of Low Stage CLL Identify WNT/PCP, Flt-3L/Flt-3 and CXCL9/CXCR3 as Regulators of Cell Proliferation, Survival and MigrationHuman genomics and proteomics : HGP20981323CD19+ normal PB B-cell RNA from 4 normal volunteers was purchased from AllCells, CA. Control RNA was obtained from normal peripheral (PB) B-cells from 4 volunteers (AllCells, CA) SaveFull Paper
2009Garzon, R;MicroRNA profiling of megakaryocytesMethods in molecular biology (Clifton, N.J.)18839118It is critical to use high-quality CD34+ stem cells. These cells could be obtained commercially from Allcells.com or Stemcell technologies. Other cheaper alternatives include the use of cord blood-selected CD34+ cells. Failure to use high-quality proFull Paper
2009Huynh, H;Kaba, M;Rudra, S;Zheng, J;Wu, C;Lodish, H;Zhang, C;IGFBP2 Supports ex vivo Expansion of Hematopoietic Stem CellsResearch and Perspectives in Endocrine InteractionsFresh and cryopreserved human cord blood cells were purchased from Cambrex, StemCell Technologies Inc., and AllCells. All of the cells were from pooled donors. CD34 + or CD133 SaveFull Paper
2009Harirchian, P;Validation of EBI2 as a Novel Therapeutic Target for Autoimmune DisordersThesisFresh leukapheresed blood (300 mL) from healthy donors was purchased from Allcells (Emeryville, CA). On the same day of leukapheresis, peripheral blood mononuclear cells (PBMC) SaveFull Paper
2009Bachstetter, AD;The role of neuroinflammation in regulating the age-related decline in neurogenesisThesisCell preparation: Cryopreserved human umbilical cord blood mononuclear cells (UCBMCs) were obtained from Saneron CCEL Therapeutics, Inc. (Tampa, FL, USA). Cryopreserved Human peripheral blood cells (PBMC) (mononuclear fraction) were obtained from AllFull Paper
2009Carter, CC;Infection of hematopoietic progenitor cells by HIV-1ThesisCell culture. Fresh whole bone marrow aspirates were obtained commercially (AllCells Ltd.). Umbilical cord blood was obtained following scheduled cesarean section procedures, in accordance with an IRB-approved protocol. Bone marrow mononuclear cellsFull Paper
2009O'Brien, JJ;Mechanisms of megakaryopoiesis and platelet production: Implications for inflammation and diseaseThesisHuman CD34+ cord blood cells were obtained from AllCells (Emeryville, CA). Cells were plated at 2.5x10" cells per well in a 12-well plate and cultured in serum-free medium as previously described (Zeuner et al. 2007) and supplemented with 100 ng/mL oFull Paper
2009Hall, AA;Immunomodulatory effects of novel therapies for strokeThesisDMEM without glucose (Invitrogen, Carlsbad, CA) for OGD. HUCBC (AllCells) were thawed and resuspended in DMEM containing DNase (1ug/ml) to prevent aggregation. HUCBC were washed twice and resuspended at a concentration of 108 cells/ml. HUCBC (106 )Full Paper
2009Schwengberg, S;Use of embryonic stem cell derived cardiomyocytes for determination of cardiac cytotoxicityJournal of Pharmacological and Toxicological MethodsHescheler for providing Patch Clamp analysis; Leonard Fink for development and programming of the automated analysis software; our colleagues at Axiogenesis AG and ReachBio SaveFull Paper
2008Ramalingam, S;Forster, J;Naret, C;Evans, T;Sulecki, M;Lu, H;Teegarden, P;Weber, MR;Belani, CP;Dual inhibition of the epidermal growth factor receptor with cetuximab, an IgG1 monoclonal antibody, and gefitinib, a tyrosine kinase inhibitor, in patients with refractory non-small cell lung cancer (NSCLC): a phase I studyJ Thorac Oncol1831706838. FFPE tumor samples were submitted to QualTek (Newtown, PA) for EGFR expression analysis by immunohistochemistry. The intensity of staining was scored as: 3 = high, 2 = moderate, 1 = low/minimal, and 0 = no staining. RESULTS. Patient DemographicsFull Paper
2008Jiang, P;Enomoto, A;Jijiwa, M;Kato, T;Hasegawa, T;Ishida, M;Sato, T;Asai, N;Murakumo, Y;Takahashi, M;An actin-binding protein Girdin regulates the motility of breast cancer cellsCancer Res.18316593Tissue arrays of human malignant tumors and matched normal adjacent tissues were purchased from Folio Biosciences, Inc. Immunohistochemisty was performed using standard techniques. Antigen retrieval was performed with microwave treatment in a 0.01 moFull Paper
2008Lu, Y;Wang, J;Xu, Y;Koch, AE;Cai, Z;Chen, X;Galson, DL;Taichman, RS;Zhang, J;CXCL16 functions as a novel chemotactic factor for prostate cancer cells in vitroMol. Cancer Res.18344492High-density tissue microarrays were constructed by Folio Biosciences with clinical samples obtained from a cohort of 80 patients. Tumors were graded using the Gleason grading system and examined to identify areas of benign prostate, prostate cancer,Full Paper
2008Wang, J;Shiozawa, Y;Wang, J;Wang, Y;Jung, Y;Pienta, KJ;Mehra, R;Loberg, R;Taichman, RS;The role of CXCR7/RDC1 as a chemokine receptor for CXCL12/SDF-1 in prostate cancerJ. Biol. Chem.18057003tumor size, or clinical outcome. Breast cancer arrays were purchased from Folio Biosciences (Columbus, OH) (ARY-HH0058), which were generated from clinical samples derived from a cohort of 80 patients. In this case anFull Paper
2008Gallagher, J;Lynch, FW;Barragry, J;A prolactinoma masked by a herbal remedyEur. J. Obstet. Gynecol. Reprod. Biol.17298863Full Paper
2008Meszaros, R;Strate, I;Pera, EM;Durbeej, M;Expression of the novel gene Ened during mouse and Xenopus embryonic developmentInt. J. Dev. Biol.18956345Mul- tiple alignment of the amino acid sequences was carried out with MultAlin software (http://bioinfo.genopole-toulouse.prd.fr/multalin/multalin.html). Whole-mount and radioactive in situ hybridization (Phylogeny, Inc. ColumbusFull Paper
2008Kawada, M;Inoue, H;Arakawa, M;Ikeda, D;Transforming growth factor-β1 modulates tumor-stromal cell interactions of prostate cancer through insulin-like growth factor-IAnticancer researchImmunohistochemistry. Tissue arrays of human prostate cancer were purchased from Folio Biosciences (Columbus, OH, USA). The tissue sections were deparaffinized in xylene and rehydrated in descending alcohols into waterFull Paper
2008Cherry, RA;Nichols, PA;Snavely, TM;David, MT;Lynch, FC;Prophylactic inferior vena cava filters: do they make a difference in trauma patients?J Trauma18784566Full Paper
2008Yang, L;Anderson, DE;Baecher-Allan, C;Hastings, WD;Bettelli, E;Oukka, M;Kuchroo, VK;Hafler, DA;IL-21 and TGF-beta are required for differentiation of human T(H)17 cellsNature18469800PBMCs were obtained from the peripheral blood of healthy subjects or from cord blood (AllCells) in compliance with institutional review board (IRB) protocols. CD4 + T cells were SaveFull Paper
2008Cisse, B;Caton, ML;Lehner, M;Maeda, T;Scheu, S;Locksley, R;Holmberg, D;Zweier, C;den Hollander, NS;Kant, SG;Holter, W;Rauch, A;Zhuang, Y;Reizis, B;Transcription factor E2-2 is an essential and specific regulator of plasmacytoid dendritic cell developmentCell18854153Total RNA from purified human peripheral blood cell types (Allcells) or sorted murine cells was reverse transcribed and assayed by SYBR Green-based real-time PCR with MX3000P SaveFull Paper
2008Paoluzzi, L;Gonen, M;Bhagat, G;Furman, RR;Gardner, JR;Scotto, L;Gueorguiev, VD;Heaney, ML;Manova, K;O'Connor, OA;The BH3-only mimetic ABT-737 synergizes the antineoplastic activity of proteasome inhibitors in lymphoid malignanciesBlood18591385Mononuclear cells from peripheral blood samples (PBMCs) of healthy donors were purchased from Allcells (Emeryville, CA). All cell lines were grown as previously described. SaveFull Paper
2008Luqman, M;Klabunde, S;Lin, K;Georgakis, GV;Cherukuri, A;Holash, J;Goldbeck, C;Xu, X;Kadel, EE;Lee, SH;Aukerman, SL;Jallal, B;Aziz, N;Weng, WK;Wierda, W;O'Brien, S;Younes, A;The antileukemia activity of a human anti-CD40 antagonist antibody, HCD122, on human chronic lymphocytic leukemia cellsBlood18497318B-cell chronic lymphocytic leukemia (B-CLL) is a lymphoproliferative disorder characterized by the surface expression of CD20, CD5 antigens, as well as the receptor CD40. Activation SaveFull Paper
2008Zhang, CC;Kaba, M;Iizuka, S;Huynh, H;Lodish, HF;Angiopoietin-like 5 and IGFBP2 stimulate ex vivo expansion of human cord blood hematopoietic stem cells as assayed by NOD/SCID transplantationBlood18202223Fresh and cryopreserved human cord blood cells were purchased from Cambrex (East Rutherford, NJ), StemCell Technologies, and AllCells (Berkeley, CA). All of cells were from pooled donors. CD34+ or CD133+ purity checked by flow cytometry was higher thFull Paper
2008Garzon, R;Volinia, S;Liu, CG;Fernandez-Cymering, C;Palumbo, T;Pichiorri, F;Fabbri, M;Coombes, K;Alder, H;Nakamura, T;Flomenberg, N;Marcucci, G;Calin, GA;Kornblau, SM;Kantarjian, H;Bloomfield, CD;Andreeff, M;Croce, CM;MicroRNA signatures associated with cytogenetics and prognosis in acute myeloid leukemiaBlood18187662from 4 healthy donors were purchased from Allcells (Emeryville, CA). Bone marrow CD34 + progenitors from 10 healthy donors were purchased from Allcells. In vitro differentiated SaveFull Paper
2008Mercher, T;Cornejo, MG;Sears, C;Kindler, T;Moore, SA;Maillard, I;Pear, WS;Aster, JC;Gilliland, DG;Notch signaling specifies megakaryocyte development from hematopoietic stem cellsCell stem cell18786418using a FACSAria (BD Biosciences). Human CD34+ bone marrow cells were obtained from AllCells. For in vitro cocultures of LSK cells with stroma, analyses were gated on forward/side scatter profile and CD45+ or CD45+GFP+ cells. Apoptosis assays were peFull Paper
2008Tuller, T;Kupiec, M;Ruppin, E;Evolutionary rate and gene expression across different brain regionsGenome biology18811952names and other properties appears in Additional data file 12. The human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA, USA), Clontech (Palo Alto, CA, USA), AllCells (Berkeley, CA, USA), Clonetics/BioWhittakFull Paper
2008Song, W;van der Vliet, HJ;Tai, YT;Prabhala, R;Wang, R;Podar, K;Catley, L;Shammas, MA;Anderson, KC;Balk, SP;Exley, MA;Munshi, NC;Generation of antitumor invariant natural killer T cell lines in multiple myeloma and promotion of their functions via lenalidomide: a strategy for immunotherapyClinical cancer research : an official journal of the American Association for Cancer Research18980990Normal bone marrow samples were purchased from AllCells. Multiple myeloma patient blood and bone marrow samples were obtained from Dana-Farber Cancer Institute following SaveFull Paper
2008Lu, J;Guo, S;Ebert, BL;Zhang, H;Peng, X;Bosco, J;Pretz, J;Schlanger, R;Wang, JY;Mak, RH;Dombkowski, DM;Preffer, FI;Scadden, DT;Golub, TR;MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte progenitorsDevelopmental cell18539114Human umbilical cord blood was harvested at Brigham and Women’s Hospital with informed patient consent under an IRB approved protocol. Adult human bone marrow cells were obtained from AllCells, LLC. Mononuclear cells were purified by Ficoll Hypaque sFull Paper
2008Solomon, DA;Kim, JS;Cronin, JC;Sibenaller, Z;Ryken, T;Rosenberg, SA;Ressom, H;Jean, W;Bigner, D;Yan, H;Samuels, Y;Waldman, T;Mutational inactivation of PTPRD in glioblastoma multiforme and malignant melanomaCancer research19074898Normal human astrocytes (NHA) were obtained from Clonetics and AllCells. All cell lines were grown in DMEM + 10% fetal bovine serum (FBS) at 37C in 5% CO 2 . SaveFull Paper
2008Lau, YK;Murray, LB;Houshmandi, SS;Xu, Y;Gutmann, DH;Yu, Q;Merlin is a potent inhibitor of glioma growthCancer research18632626Normal human astrocytes (NHA cells) were obtained from ALLCELLS, Inc. Cells were maintained following the providers' and manufacturers' instructions. Anti-v5 epitope (Invitrogen), SaveFull Paper
2008Solomon, DA;Kim, JS;Jenkins, S;Ressom, H;Huang, M;Coppa, N;Mabanta, L;Bigner, D;Yan, H;Jean, W;Waldman, T;Identification of p18 INK4c as a tumor suppressor gene in glioblastoma multiformeCancer research18381405Normal human astrocytes (NHAs) were obtained from Clonetics and Allcells. All cell lines were grown in DMEM + 10% fetal bovine serum at 37 in 5% CO 2 . SaveFull Paper
2008Bonini, MG;Stadler, K;Silva, SO;Corbett, J;Dore, M;Petranka, J;Fernandes, DC;Tanaka, LY;Duma, D;Laurindo, FR;Mason, RP;Constitutive nitric oxide synthase activation is a significant route for nitroglycerin-mediated vasodilationProceedings of the National Academy of Sciences of the United States of America18562300eNOS (Ser-1177 and Thr-495) were from Santa Cruz and used according to the manufacturer's instructions. HUVECs were from Allcells and cultured as described (24). All other chemicals used were analytical grade or superiorFull Paper
2008O'Hare, T;Eide, CA;Tyner, JW;Corbin, AS;Wong, MJ;Buchanan, S;Holme, K;Jessen, KA;Tang, C;Lewis, HA;Romero, RD;Burley, SK;Deininger, MW;SGX393 inhibits the CML mutant Bcr-AblT315I and preempts in vitro resistance when combined with nilotinib or dasatinibProceedings of the National Academy of Sciences of the United States of America18367669Hematopoietic Colony Forming Assays of Normal Bone Marrow. Bone marrow mononuclear cells (AllCells) from three normal individuals were cultured with SGX393. Cells (5 × 104) were plated in methylcellulose containing 50 ng/ml SCF, 10 ng/ml GM-CSF, andFull Paper
2008Lund, TC;Tolar, J;Orchard, PJ;Granulocyte colony-stimulating factor mobilized CFU-F can be found in the peripheral blood but have limited expansion potentialHaematologica18403392Briefly, blood from G-CSF treated healthy volunteers was purchased from AllCells, LLC (Emeryville, CA, USA) (see Online Supplementary Appendix). The use of all tissue was approved by the Committee on the Use of Human Subjects in Research at the UniveFull Paper
2008Van Scott, MR;Mertsching, E;Negrou, E;Miles, J;Stallings, HW;Graff, C;Kehry, MR;Systemic administration of an Fcgamma-Fc(epsilon)-fusion protein in house dust mite sensitive nonhuman primatesClinical immunology (Orlando, Fla.)18583194Human basophils were differentiated from CD34+ cord blood progenitors (AllCells, LLC, Emeryville, CA) by 4- to 5-weeks of culture in complete medium [RPMI 1640 (ATCC, Manassas, VA), 10% FBS (HyClone, Logan , UT), 10 μg/ml gentamicin (Sigma-Aldrich, SFull Paper
2008Reyna, SM;Ghosh, S;Tantiwong, P;Meka, CS;Eagan, P;Jenkinson, CP;Cersosimo, E;Defronzo, RA;Coletta, DK;Sriwijitkamol, A;Musi, N;Elevated toll-like receptor 4 expression and signaling in muscle from insulin-resistant subjectsDiabetes18633101e performed Western analysis and real-time PCR of EMR1, a marker for monocyte/macrophage infiltration, in muscle tissue of insulin-resistant subjects and in cultured myotubes. Unlike CD14+ monocytes (AllCells, Emeryville, CA), which expressed EMR1, nFull Paper
2008Smith, LM;Nesterova, A;Ryan, MC;Duniho, S;Jonas, M;Anderson, M;Zabinski, RF;Sutherland, MK;Gerber, HP;Van Orden, KL;Moore, PA;Ruben, SM;Carter, PJ;CD133/prominin-1 is a potential therapeutic target for antibody-drug conjugates in hepatocellular and gastric cancersBritish journal of cancer18542072idoma AC133. 1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and normal human primary cells (HREC, hepatocytes) were obtained from Cambrex (Lonza, Switzerland) and AllCells (Emeryville, CA, USA), respectively. CellFull Paper
2008Xu, Y;Theobald, V;Sung, C;DePalma, K;Atwater, L;Seiger, K;Perricone, MA;Richards, SM;Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patientsJournal of translational medicine18945350oulter Immunomics, San Diego, CA). Frozen PBMC Samples: Frozen peripheral blood mononuclear cells (PBMCs), screened HIV negative, were used in this study. PBMC from blood of HLA-A2 healthy subjects (AllCells, LLC, Emeryville, CA and American Red CroFull Paper
2008DiBlasio-Smith, EA;Arai, M;Quinet, EM;Evans, MJ;Kornaga, T;Basso, MD;Chen, L;Feingold, I;Halpern, AR;Liu, QY;Nambi, P;Savio, D;Wang, S;Mounts, WM;Isler, JA;Slager, AM;Burczynski, ME;Dorner, AJ;LaVallie, ER;Discovery and implementation of transcriptional biomarkers of synthetic LXR agonists in peripheral blood cellsJournal of translational medicine18925943Fresh human PBMC, T cells, B cells, and monocytes from normal human donors were purchased from AllCells (Emeryville, CA). Each cell set was derived from the same donor for SaveFull Paper
2008Inamdar, KV;O'Brien, S;Sen, S;Keating, M;Nguyen, MH;Wang, X;Fernandez, M;Thomazy, V;Medeiros, LJ;Bueso-Ramos, CE;Aurora-A kinase nuclear expression in chronic lymphocytic leukemiaModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc18931650fam-ATCAAGATCATTGCTCCTCCTGAGCGC-bhq) at 100 nM concentrations. Real-time quantitative RT-PCR was performed in an automatic MX3000P real-time RT-PCR thermal cycler (Stratagene, La Jolla, CA, USA). Relative RNA level was reported via standard delta delFull Paper
2008Peterson, SJ;Drummond, G;Kim, DH;Li, M;Kruger, AL;Ikehara, S;Abraham, NG;L-4F treatment reduces adiposity, increases adiponectin levels, and improves insulin sensitivity in obese miceJournal of lipid research18426778Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing, mononuclear cells were resuspended in α-MEM (Invitrogen) supplemented with 10% heat-inactivated FBS (Invitrogen), and 1% antibiotic/antimycotic solutioFull Paper
2008Liebler, JM;Lutzko, C;Banfalvi, A;Senadheera, D;Aghamohammadi, N;Crandall, ED;Borok, Z;Retention of human bone marrow-derived cells in murine lungs following bleomycin-induced lung injuryAmerican journal of physiology. Lung cellular and molecular physiology18515407Human BMDC preparation and administration. Whole adult human bone marrow cells (Cambrex, Walkersville, MD or AllCells, Emeryville, CA) were used as the source of BMDC under a protocol approved by the Institutional Review Board of the University of SoFull Paper
2008Mina-Osorio, P;Winnicka, B;O'Conor, C;Grant, CL;Vogel, LK;Rodriguez-Pinto, D;Holmes, KV;Ortega, E;Shapiro, LH;CD13 is a novel mediator of monocytic/endothelial cell adhesionJournal of leukocyte biology1849578837 cells, WEHI‐274.1 (murine myeloid cell line), C33A cells, and hemangioendothelioma (EOMA) cells [19] were from the American Type Cell Culture Collection (ATCC; Manassas, VA, USA). PBMC were from AllCells (Emeryville, CA, USA). Anti‐CD13 mAb 452 anFull Paper
2008Kopetzki, E;Jekle, A;Ji, C;Rao, E;Zhang, J;Fischer, S;Cammack, N;Sankuratri, S;Heilek, G;Closing two doors of viral entry: intramolecular combination of a coreceptor- and fusion inhibitor of HIV-1Virology journal18452606s were obtained from the NIH AIDS Research and Reference Reagent program. Human embryonic kidney cells 293-T and HEK293-EBNA cells were obtained from ATCC, Manassas, VA. Human PBMC were obtained from AllCells (Emeryville, CA), stimulated for one dayFull Paper
2008Tomchuck, SL;Zwezdaryk, KJ;Coffelt, SB;Waterman, RS;Danka, ES;Scandurro, AB;Toll-like receptors on human mesenchymal stem cells drive their migration and immunomodulating responsesStem cells (Dayton, Ohio)17916800Human MSCs were obtained from our collaborators at the Tulane University Center for Gene Therapy led by Darwin J. Prockop, M.D., Ph.D. Additionally, MSCs were obtained from two commercial suppliers Cambrex BioScience (Walkersville, MD) and Allcells (Full Paper
2008Lee, EJ;Baek, M;Gusev, Y;Brackett, DJ;Nuovo, GJ;Schmittgen, TD;Systematic evaluation of microRNA processing patterns in tissues, cell lines, and tumorsRNA (New York, N.Y.)18025253Human cancer cell lines were obtained from ATCC or other investigators. Human total RNA from 20 tissues was from Ambion. CD19+ human B cells were purchased from AllCells. RNA from pancreas and liver tumor and adjacent benign pancreas were obtained frFull Paper
2008Clark, PA;Moioli, EK;Sumner, DR;Mao, JJ;Porous implants as drug delivery vehicles to augment host tissue integrationFASEB journal : official publication of the Federation of American Societies for Experimental Biology18198217Fresh bone marrow samples of multiple adult male donors (AllCells, Berkeley, CA, USA) were used to isolate MSC, per our previous methods (19, 23). Nonadherent cells were removed SaveFull Paper
2008Mittal, R;Prasadarao, NV;Outer membrane protein A expression in Escherichia coli K1 is required to prevent the maturation of myeloid dendritic cells and the induction of IL-10 and TGF-betaJournal of immunology (Baltimore, Md. : 1950)18684958Dendritic cells (DCs) are professional APCs that direct both cellular and humoral immune responses. Escherichia coli K1 causes meningitis in neonates; however, the interactions SaveFull Paper
2008Fukaya, Y;Shimada, H;Wang, LC;Zandi, E;DeClerck, YA;Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stromaThe Journal of biological chemistry18450743transferrin (0.25 μg/ml), selenium (0.25 ng/ml), and l-glutamine. Human BMSC were obtained from ALLCELLS (Emeryville, CA) and maintained in basal medium with stimulatory supplements containing basic fibroblast growth factor (10 ng/ml)Full Paper
2008Tong, Q;Hirschler-Laszkiewicz, I;Zhang, W;Conrad, K;Neagley, DW;Barber, DL;Cheung, JY;Miller, BA;TRPC3 is the erythropoietin-regulated calcium channel in human erythroid cellsThe Journal of biological chemistry18276585Human erythroid progenitors/precursors were cultured using a two-phase liquid culture system (31, 32). Cells harvested at day 8 of Phase II were predominantly proerythroblasts and basophilic normoblasts. CD34+ cells were purchased from AllCells, LLCFull Paper
2008Wang, M;Windgassen, D;Papoutsakis, ET;Comparative analysis of transcriptional profiling of CD3+, CD4+ and CD8+ T cells identifies novel immune response players in T-cell activationBMC genomics18485203ng regulation of T-cell activation in the natural CD3+ population, as well as in the CD4+ and CD8+ subsets. Methods Cells and culture system Healthy-donor peripheral blood mononuclear cells (PBMCs) (AllCells, Berkeley, CA) were used to negatively-seFull Paper
2008Moioli, EK;Clark, PA;Sumner, DR;Mao, JJ;Autologous stem cell regeneration in craniosynostosisBone18023269Human mesenchymal stem cells isolated from bone marrow (AllCells, Berkeley, CA) were cultured in 6-well plates (3 × 10 4 cells/well) with osteogenic supplements as described above SaveFull Paper
2008Liang, W;Rahaman, M;Day, D;Marion, N;Riley, G;Mao, J;Bioactive borate glass scaffold for bone tissue engineeringJournal of Non-Crystalline SolidsBone marrow derived human mesenchymal stem cells, hbMSCs (AllCells; Berkeley, CA) were cultured for 18 days in either DMEM complete (89% DMEM 10% FBS, 1% penicillin - SaveFull Paper
2008Sikorska, M;Sandhu, JK;Deb-Rinker, P;Jezierski, A;Leblanc, J;Charlebois, C;Ribecco-Lutkiewicz, M;Bani-Yaghoub, M;Walker, PR;Epigenetic modifications of SOX2 enhancers, SRR1 and SRR2, correlate with in vitro neural differentiationJournal of neuroscience research18293417Primary human astrocytes (HA; prepared from fetuses between 9 and 10 week of gestation) were purchased from AllCells LLC. (Emeryville, CA). The cells were cultured in poly-L-lysine-coated T25-cm2 flasks using basal medium containing stimulatory supplFull Paper
2008Papaliodis, D;Boucher, W;Kempuraj, D;Michaelian, M;Wolfberg, A;House, M;Theoharides, TC;Niacin-induced "flush" involves release of prostaglandin D2 from mast cells and serotonin from platelets: evidence from human cells in vitro and an animal modelThe Journal of pharmacology and experimental therapeutics18784348Freshly isolated human platelets were purchased from AllCells, LLC (Emeryville, CA) and sent to us overnight. Platelets were used the day of arrival after washing as indicated below. SaveFull Paper
2008Kim, DH;Burgess, AP;Li, M;Tsenovoy, PL;Addabbo, F;McClung, JA;Puri, N;Abraham, NG;Heme oxygenase-mediated increases in adiponectin decrease fat content and inflammatory cytokines tumor necrosis factor-alpha and interleukin-6 in Zucker rats and reduce adipogenesis in human mesenchymal stem cellsThe Journal of pharmacology and experimental therapeutics18334666Frozen bone marrow mononuclear cells were purchased from Allcells (Emeryville, CA). After thawing the cells, mononuclear cells were resuspended in an α-minimal essential medium ( SaveFull Paper
2008Fuhrken, PG;Chen, C;Apostolidis, PA;Wang, M;Miller, WM;Papoutsakis, ET;Gene Ontology-driven transcriptional analysis of CD34+ cell-initiated megakaryocytic cultures identifies new transcriptional regulators of megakaryopoiesisPhysiological genomics18252802Mk and G cultures were initiated with fresh (AllCells; Berkeley, CA) or frozen (Hematopoietic Cell Processing Core, Fred Hutchinson Cancer Center, Seattle, WA) granulocyte colony-stimulating factor (G-CSF)-mobilized human peripheral blood CD34+ cellsFull Paper
2008Stirewalt, DL;Meshinchi, S;Kopecky, KJ;Fan, W;Pogosova-Agadjanyan, EL;Engel, JH;Cronk, MR;Dorcy, KS;McQuary, AR;Hockenbery, D;Wood, B;Heimfeld, S;Radich, JP;Identification of genes with abnormal expression changes in acute myeloid leukemiaGenes, chromosomes & cancer17910043Cancer Research Center (FHCRC) or purchased from commercially available vendors (Cambrex, Rutherford, NJ or AllCells, Emeryville, CA). BM (N = 7) and PB (N = 19) samples from 26 adult AML patients were obtained from the FHCRC's Leukemia Repository. TFull Paper
2008Li, YY;Bao, M;Meurer, J;Skuballa, W;Bauman, JG;Doecke, WD;Zollner, TM;The identification of a small molecule inhibitor that specifically reduces T cell-mediated adaptive but not LPS-mediated innate immunity by T cell membrane-monocyte contact bioassayImmunology letters18241931PBMCs were either purchased (Allcells, Emeryville, CA) or isolated from healthy donor blood after informed consent. Berlex environmental health & safety (EHS) policies and SaveFull Paper
2008Ramsubir, S;Nonaka, T;Girbés, CB;Carpentier, S;Levade, T;Medin, JA;In vivo delivery of human acid ceramidase via cord blood transplantation and direct injection of lentivirus as novel treatment approaches for Farber diseaseMolecular genetics and metabolism18805722CD34+ cells were obtained from AllCells, LLC (Berkeley, CA). Cells were pre-stimulated for 12 h in StemSpan SFEM medium (Stem Cell Technologies, Vancouver, BC, Canada) supplemented with recombinant human SCF, Flt3-L, thrombopoietin (all at 50 ng/ml),Full Paper
2008Huang, D;Pereboev, AV;Korokhov, N;He, R;Larocque, L;Gravel, C;Jaentschke, B;Tocchi, M;Casley, WL;Lemieux, M;Curiel, DT;Chen, W;Li, X;Significant alterations of biodistribution and immune responses in Balb/c mice administered with adenovirus targeted to CD40(+) cellsGene therapy18046426using Adeno-X Rapid Titer kit according to the manufacturer's instructions (BD Biosciences, San Jose, CA, USA). DC culture, Ad infection and luciferase activity assay Myeloid DCs were obtained from AllCELLS, Emeryville, CA, USA. Over 80% of these moFull Paper
2008Oh, W;Kim, DS;Yang, YS;Lee, JK;Immunological properties of umbilical cord blood-derived mesenchymal stromal cellsCellular immunology18495100To assess T-cell reactivity against allogeneic cell populations, human responder PBMCs (1 × 105/well) were cocultured with inactivated allogeneic PBMCs (1 × 105/well) or hUCB-MSCs (1 × 102, 1 × 103 or 1 × 104/well) in 96-well tissue culture plates. TFull Paper
2008Seo, BM;Sonoyama, W;Yamaza, T;Coppe, C;Kikuiri, T;Akiyama, K;Lee, JS;Shi, S;SHED repair critical-size calvarial defects in miceOral diseases18938268Human bone marrow cells were purchased from commercially available resources (AllCells LLC, Berkeley, CA, USA). To identify putative bone marrow mesenchymal stem cells, single SaveFull Paper
2008Moioli, EK;Clark, PA;Chen, M;Dennis, JE;Erickson, HP;Gerson, SL;Mao, JJ;Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissuesPloS one19081793Bone marrow cells were isolated from whole marrow aspirates of the iliac crest of each of three healthy male donors (AllCells, Berkeley, CA) and plated as in Fig. 1A. Human SaveFull Paper
2008Yamaza, T;Miura, Y;Bi, Y;Liu, Y;Akiyama, K;Sonoyama, W;Patel, V;Gutkind, S;Young, M;Gronthos, S;Le, A;Wang, CY;Chen, W;Shi, S;Pharmacologic stem cell based intervention as a new approach to osteoporosis treatment in rodentsPloS one18612428amethasone (Sigma-Aldrich Co.) were added into the culture for 6 weeks. Human BMMSC culture Human bone marrow aspirates from healthy human adult volunteers (20-35 years of age) were purchased from AllCells LLC and cultured as reported previously [21Full Paper
2008Ghosh, A;Park, JY;Fenno, C;Kapila, YL;Porphyromonas gingivalis, gamma interferon, and a proapoptotic fibronectin matrix form a synergistic trio that induces c-Jun N-terminal kinase 1-mediated nitric oxide generation and cell deathInfection and immunity18838522Human primary normal peripheral blood neutrophils (AllCells, CA) were maintained in Iscove's modified Dulbecco's medium containing 5% fetal bovine serum, 2 mM glutamine, 25 mM SaveFull Paper
2008Hanten, JA;Vasilakos, JP;Riter, CL;Neys, L;Lipson, KE;Alkan, SS;Birmachu, W;Comparison of human B cell activation by TLR7 and TLR9 agonistsBMC immunology186526793') was obtained from (Genosys, Woodlands, TX). Isolation and treatment of human peripheral blood B cells Human peripheral blood mononuclear cells (PBMCs) were obtained from healthy volunteers from AllCells, LLC. (Berkeley, CA) and Memorial Blood CeFull Paper
2008López, MC;Lawrence, DA;Proficiency testing experience for viable CD34+ stem cell analysisTransfusion18298596Human peripheral blood was collected in blood collection tubes (Cyto-Chex BCT, Streck Laboratories, La Vista, NE), which contain a preservative reagent for flow cytometry. Frozen CD34+ stem cells from cord blood (AllCells LLC, Berkeley, CA) were thawFull Paper
2008Tang, H;Lu, JY;Zheng, X;Yang, Y;Reagan, JD;The psoriasis drug monomethylfumarate is a potent nicotinic acid receptor agonistBiochemical and biophysical research communications187223462B were purchased from AllCells (Emeryville, CA) and were of greater than 95% purity. Human CD14+ monocytes and NHEK were from Cambrex (East Rutherford, NJ) and cultured as SaveFull Paper
2008Bachstetter, AD;Pabon, MM;Cole, MJ;Hudson, CE;Sanberg, PR;Willing, AE;Bickford, PC;Gemma, C;Peripheral injection of human umbilical cord blood stimulates neurogenesis in the aged rat brainBMC neuroscience18275610cord blood mononuclear cells (UCBMCs) were obtained from Saneron CCEL Therapeutics, Inc. (Tampa, FL, USA). Cryopreserved Human peripheral blood cells (PBMC) (mononuclear fraction) were obtained from AllCells, LLC (Emeryville, CA, USA). Just prior toFull Paper
2008Mashiyama, ST;Hansen, CM;Roitman, E;Sarmiento, S;Leklem, JE;Shultz, TD;Ames, BN;An assay for uracil in human DNA at baseline: effect of marginal vitamin B6 deficiencyAnalytical biochemistry17963712, respectively, in measuring DNA-uracil in all the samples used in experiments described in this paper (lymphocytes from the B6 deficiency study and lymphocytes from AllCells). Uracil SaveFull Paper
2008Park, EJ;Kim, ES;Weber, HP;Wright, RF;Mooney, DJ;Improved bone healing by angiogenic factor-enriched platelet-rich plasma and its synergistic enhancement by bone morphogenetic protein-2The International journal of oral & maxillofacial implants19014150Fresh human adult whole blood was purchased from AllCells (Emeryville, CA, USA). Peripheral blood mononuclear cells (PBMNCs) and platelets were separately isolated by a density gradient centrifugation (Histopaque-1077, Sigma, St. Louis, MO, USA) accoFull Paper
2008Thamm, DH;O'Brien, MG;Vail, DM;Serum vascular endothelial growth factor concentrations and postsurgical outcome in dogs with osteosarcomaVeterinary and comparative oncology19178672Vascular endothelial growth factor (VEGF) is a key angiogenic growth factor, playing putative roles in both tumour growth and metastasis. The purpose of this study was to correlate SaveFull Paper
2008Antonescu, CR;Wu, K;Xing, GL;Cao, M;Turpaz, Y;Leversha, MA;Hubbell, E;Maki, RG;Miyada, CG;Pillai, R;DNA copy number analysis in gastrointestinal stromal tumors using gene expression microarraysCancer informatics19259404from the American Type Culture Collection (ATCC, Manassas, VA). DNA was extracted from the cultured cells using the DNA Maxi Kit (Qiagen, Inc., Valencia, CA). DNA from normal blood was obtained from AllCells, LLC (Emeryville, CA). GIST sample DNA wasFull Paper
2008Li, M;Rossi, JJ;Lentiviral vector delivery of siRNA and shRNA encoding genes into cultured and primary hematopoietic cellsMethods in molecular biology (Clifton, N.J.)18679631Target cells: K562 cells, a chronic myelogenous leukemia cell line (ATCC, #CCL-243). CD34+ hematopoietic stem cells, purchased from AllCells (Berkley, CA, USA) or purified from umbilical cord blood or bone marrow by anti-CD34 antibody-coupled magnetiFull Paper
2008Campbell-Anson, RE;Kentor, D;Wang, YJ;Bushnell, KM;Li, Y;Vence, LM;Radvanyi, LG;A new approach for the large-scale generation of mature dendritic cells from adherent PBMC using roller bottle technologyJournal of immune based therapies and vaccines18321390Sources of PBMC for DC generation PBMC were obtained from peripheral blood leukopheresis products obtained from non-mobilized normal donors (LifeBlood, Memphis, TN), or G-CSF-mobilized normal donors (AllCells, Berkeley, CA). Products were collected iFull Paper
2008Tiainen, J;Structured equity products and portfolio compositions of financially advised investorsThesisRESULTS I will show in this thesis that behavioural biases of the retail investors are associated to proportions of portfolio invested in structured capital guaranteed products and in equity SaveFull Paper
2008Shi, M;The Role of Janus-Kinase-3 in CD4+ T Cell Proliferation and Differentiation: A DissertationThesisAliquots of cryopreserved peripheral blood mononuclear cells (PBMC) were purchased from AllCells (Emeryville, CA). PBMC were resuspended in complete RPMI 1640 medium (RPMI 1640 medium supplemented with 10% fetal bovine serum, penicillin/streptomycin,Full Paper
2008Ziegler, LA;Targeting lentivector gene delivery to hematopoietic stem and progenitor cells in vivoThesisFresh, unfractionated human PBMCs (2 x 106 ) (AllCells) were incubated with concentrated virus with total TUs of 10 x 106 (based on the titer on 293T/CD20 cells). LPS (50 μg/ml) was then added for B cells to survive and grow. After 2 days, cells werFull Paper
2008Wang, M;Genomic-scale transcriptional analysis of T-cell activation reveals novel genes and signaling programsThesisHealthy-donor peripheral blood mononuclear cells (PBMCs) (AllCells, Berkeley, CA) were used to negatively-select CD3+ T cells, CD4+ and CD8+ T cells (Pan T Cell Isolation Kit II, CD4+ T Cell Isolation Kit II, and CD8+ T Cell Isolation Kit II, MiltenyFull Paper
2008Bedi, R;Characterization of the activities of the human C/EBPϵ activator and repressor isoforms in myelopoiesisThesis25 ml/cm2 . After preloading and before prestimulation, the plates were washed with PBS to remove any inhibitory molecules in the viral supernatant. Cord blood CD34+ cells purchased from AllCells (CA, USA) were thawed according to the supplier's instFull Paper
2007Aziz, MH;Manoharan, HT;Church, DR;Dreckschmidt, NE;Zhong, W;Oberley, TD;Wilding, G;Verma, AK;Protein kinase Cepsilon interacts with signal transducers and activators of transcription 3 (Stat3), phosphorylates Stat3Ser727, and regulates its constitutive activation in prostate cancerCancer Res.17875724Prostate cancer specimens were obtained at radical prostatectomy. The University of Wisconsin Human Subjects Committee approved the use of these human prostate specimens. Tissue microarrays were obtained from Folio Biosciences.4 Tissue microarrays weFull Paper
2007Senzer, N;Nemunaitis, J;Nemunaitis, M;Lamont, J;Gore, M;Gabra, H;Eeles, R;Sodha, N;Lynch, FJ;Zumstein, LA;Menander, KB;Sobol, RE;Chada, S;p53 therapy in a patient with Li-Fraumeni syndromeMol. Cancer Ther.17483435Full Paper
2007Gagna, CE;Clark Lambert, W;Cell biology, chemogenomics and chemoproteomics - application to drug discoveryExpert Opin Drug Discov23484648TMAs have been expensive and limited in terms of commercial availability [8,56-59]. Only recently have they surfaced commercially on a large scale (e.g., Cytomyx, Folio Biosciences, Chemicon). Many different types of high quality TMAs now exist, suchFull Paper
2007Flores, J;Cepeda, IL;Cornfeldt, ML;O'Kusky, JR;Doudet, DJ;Characterization and survival of long-term implants of human retinal pigment epithelial cells attached to gelatin microcarriers in a model of Parkinson diseaseJ. Neuropathol. Exp. Neurol.17620984All primary antibodies were previously assessed for hRPE cell specificity in human RPE cultured monolayer and RPE-choroid eye tissue, and for nonspecificity to striatal rat tissue (Dr. S. Bernstein, QualTek Molecular Laboratories, Santa Barbara, CA,Full Paper
2007Lynch, FC;Use of a covered stent for percutaneous transrenal occlusion of the ureterJ Vasc Interv Radiol18004001Full Paper
2007Cantwell, CP;Lynch, FC;Caudal migration of a G2 filter during carbon dioxide cavographyJ Vasc Interv Radiol17538152Full Paper
2007Lee, S;Sha, Q;Wu, X;Calenda, G;Peng, J;Expression profiles of mouse Kell, XK, and XPLAC mRNAJ. Histochem. Cytochem.17189525For the ISH experiments, the custom service from Phylogeny Inc. (Columbus, OH) was used (Lyons et al. 1990; Patapoutian et al. 1993). Briefly, tissue sections were hybridized overnight at 55C in 50% deionized formamide, 0.3 M NaCl, 20 mM Tris-HCl, pHFull Paper
2007Tanimoto, R;Abarzua, F;Sakaguchi, M;Takaishi, M;Nasu, Y;Kumon, H;Huh, NH;REIC/Dkk-3 as a potential gene therapeutic agent against human testicular cancerInt. J. Mol. Med.17273781Tissues, cells and reagents. A testicular carcinoma tissue microarray was purchased from Folio Biosciences (Columbus, OH). Surgically resected testicular cancer tissues were fixed with 4% paraformaldehyde and tissue sections were made under conventioFull Paper
2007Calin, GA;Liu, CG;Ferracin, M;Hyslop, T;Spizzo, R;Sevignani, C;Fabbri, M;Cimmino, A;Lee, EJ;Wojcik, SE;Shimizu, M;Tili, E;Rossi, S;Taccioli, C;Pichiorri, F;Liu, X;Zupo, S;Herlea, V;Gramantieri, L;Lanza, G;Alder, H;Rassenti, L;Volinia, S;Schmittgen, TD;Kipps, TJ;Negrini, M;Croce, CM;Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomasCancer cell17785203An additional set of 3 normal CD19+/CD5+ positive B cells, not used for microarray studies, was purchased from AllCells (Berkeley, CA), and used as an independent confirmation set. In all instances the qRT-PCR data confirmed the microarray data. RNAFull Paper
2007Wang, L;Wang, J;Blaser, BW;Duchemin, AM;Kusewitt, DF;Liu, T;Caligiuri, MA;Briesewitz, R;Pharmacologic inhibition of CDK4/6: mechanistic evidence for selective activity or acquired resistance in acute myeloid leukemiaBlood17537993Entry into the cell cycle is mediated by cyclin-dependent kinase 4/6 (CDK4/6) activation, followed by CDK2 activation. We found that pharmacologic inhibition of the Flt3 internal tandem SaveFull Paper
2007Mulligan, G;Mitsiades, C;Bryant, B;Zhan, F;Chng, WJ;Roels, S;Koenig, E;Fergus, A;Huang, Y;Richardson, P;Trepicchio, WL;Broyl, A;Sonneveld, P;Shaughnessy, JD;Bergsagel, PL;Schenkein, D;Esseltine, DL;Boral, A;Anderson, KC;Gene expression profiling and correlation with outcome in clinical trials of the proteasome inhibitor bortezomibBlood17185464The aims of this study were to assess the feasibility of prospective pharmacogenomics research in multicenter international clinical trials of bortezomib in multiple myeloma and to SaveFull Paper
2007Brogdon, JL;Xu, Y;Szabo, SJ;An, S;Buxton, F;Cohen, D;Huang, Q;Histone deacetylase activities are required for innate immune cell control of Th1 but not Th2 effector cell functionBlood17008546as described previously.22,23 Human neutrophils were obtained from AllCells (Berkeley, CA). Mouse bone marrow-derived DCs were prepared as described previously.24 Murine Th1 and Th2 effector cells were differentiated from DO11.10 mice as describedFull Paper
2007Mahadevan, D;Klinkhammer, T;Cooke, L;Riley, C;Choi, J;Sinari, S;Miller, T;Spier, C;CLL Transcript and Serum Profiling Identifies Distinct Oncogenic Pathways in Rai Stage Progression.BloodFull Paper
2007Robinson, S;Simmons, P;Brouard, N;Kidd, S;Yang, H;Decker, W;Xing, D;Steiner, D;Ng, J;Thomas, M;Champlin, R;de Lima, M;Hosing, C;Cooper, L;Singh, H;Zweidler-McKay, P;Shpall, E;Efficacy of ‘Off-the-Shelf’, Commercially-Available, Third-Party Mesenchymal Stem Cells (MSC) in Ex Vivo Cord Blood (CB) Co-Culture Expansion.BloodINTRODUCTION: Our previous studies have shown that clinically-relevant levels of hematopoietic stem and progenitor cell (HSPC) expansion are possible by ex vivo co-culture of cord blood (CB) mononuclear cells (MNC) with third-party bone marrow (BM)-dFull Paper
2007Majeti, R;Park, CY;Weissman, IL;Identification of a hierarchy of multipotent hematopoietic progenitors in human cord bloodCell stem cell18371405Normal human bone marrow mononuclear cells were purchased from AllCells Inc. (Emeryville, CA). Human cord blood was collected from placentas and/or umbilical cords obtained SaveFull Paper
2007Scholl, C;Bansal, D;Döhner, K;Eiwen, K;Huntly, BJ;Lee, BH;Rücker, FG;Schlenk, RF;Bullinger, L;Döhner, H;Gilliland, DG;Fröhling, S;The homeobox gene CDX2 is aberrantly expressed in most cases of acute myeloid leukemia and promotes leukemogenesisThe Journal of clinical investigation17347684and all patients and volunteers gave informed consent according to the Declaration of Helsinki. Seven of the 10 normal BMMC samples and the 3 normal BM samples that were used for isolating HSCs, CMPs, GMPs, and MEPs were purchased from AllCellsFull Paper
2007Xin, X;Hussain, M;Mao, JJ;Continuing differentiation of human mesenchymal stem cells and induced chondrogenic and osteogenic lineages in electrospun PLGA nanofiber scaffoldBiomaterials17010425Bone-marrow-derived hMSCs were purchased from AllCells (Berkeley, CA, USA). The donor was a 25 year-old healthy male volunteer without additional identifier. In our previous work, we have isolated hMSCs from multiple donors [5]. In this report, the iFull Paper
2007Zou, H;Harrington, JJ;Sugumar, A;Klatt, KK;Smyrk, TC;Ahlquist, DA;Detection of colorectal disease by stool defensin assay: an exploratory studyClinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association17531545These cDNA samples and a purchased neutrophil cNDA (AllCells, Emeryville, CA) were amplified by real-time polymerase chain reaction (PCR) in an iCycler (Bio-Rad, Hercules, CA). SaveFull Paper
2007Zhao, Y;Parkhurst, MR;Zheng, Z;Cohen, CJ;Riley, JP;Gattinoni, L;Restifo, NP;Rosenberg, SA;Morgan, RA;Extrathymic generation of tumor-specific T cells from genetically engineered human hematopoietic stem cells via Notch signalingCancer research17363559CD133 + progenitor cells were enriched from mononuclear cells from UCB (Allcells, Berkeley, CA) by depleting CD3 + cells using Dynabeads human CD3 (Invitrogen, Carlsbad, CA) SaveFull Paper
2007Verhelle, D;Corral, LG;Wong, K;Mueller, JH;Moutouh-de Parseval, L;Jensen-Pergakes, K;Schafer, PH;Chen, R;Glezer, E;Ferguson, GD;Lopez-Girona, A;Muller, GW;Brady, HA;Chan, KW;Lenalidomide and CC-4047 inhibit the proliferation of malignant B cells while expanding normal CD34+ progenitor cellsCancer research17234786Cell culture. Namalwa CSN.70 and LP-1 cells were purchased from DSMZ (Brauschweig, Germany), U266 was purchased from the American Type Culture Collection (Manassas, VA), and cord blood CD34+ cells were purchased from AllCells (Emeryville, CA). NamalwFull Paper
2007Pariente, N;Morizono, K;Virk, MS;Petrigliano, FA;Reiter, RE;Lieberman, JR;Chen, IS;A novel dual-targeted lentiviral vector leads to specific transduction of prostate cancer bone metastases in vivo after systemic administrationMolecular therapy : the journal of the American Society of Gene Therapy17653099endothelial cells were obtained from AllCells (Emeryville, CA) and grown in human umbilical vein endothelial cell basal medium plus stimulatory supplement (AllCells, Emelyville, CA). SaveFull Paper
2007Sonoyama, W;Seo, BM;Yamaza, T;Shi, S;Human Hertwig's epithelial root sheath cells play crucial roles in cementum formationJournal of dental research17586703Human bone marrow mesenchymal stem cells (BMMSCs) were purchased from a commercially available resource (AllCells LLC, Berkeley, CA, USA), and cultured as previously described (Batouli et al., 2003). Cemento/osteogenic differentiation was induced andFull Paper
2007Li, L;Piloto, O;Kim, KT;Ye, Z;Nguyen, HB;Yu, X;Levis, M;Cheng, L;Small, D;FLT3/ITD expression increases expansion, survival and entry into cell cycle of human haematopoietic stem/progenitor cellsBritish journal of haematology17359372Activating mutation of FLT3 by internal tandem duplications (ITDs) in the juxtamembrane region is the most common molecular aberration found in acute myeloid leukaemia (AML). In SaveFull Paper
2007Kikuchi, J;Shimizu, R;Wada, T;Ando, H;Nakamura, M;Ozawa, K;Furukawa, Y;E2F-6 suppresses growth-associated apoptosis of human hematopoietic progenitor cells by counteracting proapoptotic activity of E2F-1Stem cells (Dayton, Ohio)17600109magnetic cell sorting; Miltenyi Biotec, Bergisch Gladbach, Germany, http://www.miltenyibiotec.com) from human bone marrow mononuclear cells (BM-MNCs) (purchased from AllCells, Berkeley, CA, http://www.allcells.com) or cord blood mononuclear cells (CBFull Paper
2007Telford, WG;Bradford, J;Godfrey, W;Robey, RW;Bates, SE;Side population analysis using a violet-excited cell-permeable DNA binding dyeStem cells (Dayton, Ohio)17185610(FBS)/10% dimethyl sulfoxide in liquid nitrogen until use. Frozen human cord blood was obtained from AllCells LLC (Emeryville, CA, http://www.allcells.com) and thawed immediately before use. A549 lung carcinoma cells were grown in Dulbecco's modifiedFull Paper
2007Fang, D;Seo, BM;Liu, Y;Sonoyama, W;Yamaza, T;Zhang, C;Wang, S;Shi, S;Transplantation of mesenchymal stem cells is an optimal approach for plastic surgeryStem cells (Dayton, Ohio)17170063Human bone marrow cells were purchased from commercially available resources (AllCells LLC, Berkeley, CA,http://www.allcells.com/). To identify putative bone marrow MSCs, single-cell suspension of 1 × 106 of bone marrow mononuclear cells were seededFull Paper
2007Stosich, MS;Mao, JJ;Adipose tissue engineering from human adult stem cells: clinical implications in plastic and reconstructive surgeryPlastic and reconstructive surgery17255658Human mesenchymal stem cells were isolated from fresh bone marrow samples of an anonymous adult male donor (AllCells, Berkeley, Calif.) by following our previous methods. After SaveFull Paper
2007Kim, JS;Lee, C;Bonifant, CL;Ressom, H;Waldman, T;Activation of p53-dependent growth suppression in human cells by mutations in PTEN or PIK3CAMolecular and cellular biology17060456were described in a previous study (27). BJ-hTERT and RPE-hTERT cells were obtained from Clontech (Mountain View, CA). Normal human astrocytes (NHA) were obtained from AllCells LLC (Emeryville, CA) and cultured as recommended. HEK293, MCF10A, and A17Full Paper
2007Chen, C;Fuhrken, PG;Huang, LT;Apostolidis, P;Wang, M;Paredes, CJ;Miller, WM;Papoutsakis, ET;A systems-biology analysis of isogenic megakaryocytic and granulocytic cultures identifies new molecular components of megakaryocytic apoptosisBMC genomics17953764tudies of individual candidate genes. Methods Hematopoietic cultures Mk and G cell cultures were initiated with fresh or frozen G-CSF-mobilized human peripheral blood CD34+ cells from normal donors (AllCells; Berkeley, CA) and cultured as describedFull Paper
2007Vose, SC;Holland, NT;Eskenazi, B;Casida, JE;Lysophosphatidylcholine hydrolases of human erythrocytes, lymphocytes, and brain: sensitive targets of conserved specificity for organophosphorus delayed neurotoxicantsToxicology and applied pharmacology17663017Whole human blood from seven subjects and lymphocytes (isolated by aphaeresis using a leukopac filter) from three subjects were obtained from AllCells LLC (Emeryville, CA). Mouse SaveFull Paper
2007Zeng, Z;Yuan, X;Liu, G;Zeng, X;Zeng, X;Ng, H;Chen, H;Jiang, T;Akasaki, Y;Kessey, K;Black, KL;Yu, JS;Manipulation of proliferation and differentiation of human bone marrow-derived neural stem cells in vitro and in vivoJournal of neuroscience research17131390Human bone marrow cells (HBMC) were isolated from fresh human whole bone marrow (AllCells, Berkely, CA) by gradient density centrifugation (Lee et al., 2003). HBMC were plated at a density of 2 × 106 cells/ml/well onto 24-well plates (Corning, CorninFull Paper
2007Huang, LT;Paredes, CJ;Papoutsakis, ET;Miller, WM;Gene expression analysis illuminates the transcriptional programs underlying the functional activity of ex vivo-expanded granulocytesPhysiological genomics17550995of PB granulocytes should reflect their full activation potential, because RNA levels would not be expected to change very much during cell transportation and processing by AllCells (Full Paper
2007Uno, S;Kinoshita, Y;Azuma, Y;Tsunenari, T;Yoshimura, Y;Iida, S;Kikuchi, Y;Yamada-Okabe, H;Fukushima, N;Antitumor activity of a monoclonal antibody against CD47 in xenograft models of human leukemiaOncology reports17390064The ligation of CD47 induces the apoptosis of leukemic cells in a caspase-independent manner. We generated a monoclonal antibody against CD47 (mAb-MABL) that possibly induced SaveFull Paper
2007Jenkins, J;Calzone, KA;Establishing the essential nursing competencies for genetics and genomicsJournal of nursing scholarship : an official publication of Sigma Theta Tau International Honor Society of Nursing17393960129This annotation involves adding the units and labels to allcells containing no references to other cells. The systemthen analyzes the cells with formulas containing referencesand SaveFull Paper
2007Furuhata, S;Ando, K;Oki, M;Aoki, K;Ohnishi, S;Aoyagi, K;Sasaki, H;Sakamoto, H;Yoshida, T;Ohnami, S;Gene expression profiles of endothelial progenitor cells by oligonucleotide microarray analysisMolecular and cellular biochemistry17203242Among the many tissue stem or progenitor cells recently being unveiled, endothelial progenitor cells (EPCs) have attracted particular attention, not only because of their cardinal role in SaveFull Paper
2007Yourek, G;Hussain, MA;Mao, JJ;Cytoskeletal changes of mesenchymal stem cells during differentiationASAIO journal (American Society for Artificial Internal Organs : 1992)17413564Human mesenchymal stem cells were isolated from commercially available whole bone marrow (AllCells, Berkeley, CA) using RosetteSep (StemCell Technologies, Vancouver, BC) according to the manufacturer’s instructions. The RosetteSep antibody cocktail cFull Paper
2007Baysal, BE;A recurrent stop-codon mutation in succinate dehydrogenase subunit B gene in normal peripheral blood and childhood T-cell acute leukemiaPloS one1748727580°C. cDNAs synthesized from column-purified normal PBMC cell-type subsets, including monocytes (CD14+), NK cells (CD56+, negative selection), B (CD19+) and T lymphocytes (CD3+), were purchased from AllCells, LLC Inc. through StemCell Technologies. EFull Paper
2007Stosich, MS;Bastian, B;Marion, NW;Clark, PA;Reilly, G;Mao, JJ;Vascularized adipose tissue grafts from human mesenchymal stem cells with bioactive cues and microchannel conduitsTissue engineering17824832Human MSCs were isolated from fresh bone marrow samples of two anonymous adult donors (AllCells, Berkeley, CA), per our previous methods.After transferring bone marrow sample SaveFull Paper
2007Popov, BV;Serikov, VB;Petrov, NS;Izusova, TV;Gupta, N;Matthay, MA;Lung epithelial cells induce endodermal differentiation in mouse mesenchymal bone marrow stem cells by paracrine mechanismTissue engineering17630877Human hematopoietic stem cells (CD34þ) were purchased from AllCells Co. (Berkeley, CA) and confirmed to be 96% pure using FACScan. Cells were washed twice in PBS, labeled with PKH26 (Sigma) according to manufacturer’s instructions, and mixed with epiFull Paper
2007Moioli, EK;Hong, L;Mao, JJ;Inhibition of osteogenic differentiation of human mesenchymal stem cellsWound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society17537129hMSCs were isolated from donated human bone marrow samples (AllCells, Berkeley, CA) and plated in monolayers in six-well plates at a density of 3×104 cells/well per our previous methods36 (Figure 1A and B). The isolated hMSCs were cultured-expanded aFull Paper
2007Anderson, RM;Stevens, DL;Sumption, ND;Townsend, KM;Goodhead, DT;Hill, MA;Effect of linear energy transfer (LET) on the complexity of alpha-particle-induced chromosome aberrations in human CD34+ cellsRadiation research17474795Frozen human bone marrow CD34 + cells were obtained commercially (Poietics, Cambrex and AllCells, LLC) in vials of 1 × 10 6 cells (samples are anonymized and exempt from SaveFull Paper
2007Sawyer, L;Hanson, D;Castro, G;Luckett, W;Dubensky, TW;Stassinopoulos, A;Inactivation of parvovirus B19 in human platelet concentrates by treatment with amotosalen and ultraviolet A illuminationTransfusion17524098CD34+ cells isolated from granulocyte-colony-stimulating factor-mobilized human peripheral blood were obtained from AllCells (Berkeley, CA) and stimulated to proliferate and SaveFull Paper
2007Wang, TT;Tio, M;Lee, W;Beerheide, W;Udolph, G;Neural differentiation of mesenchymal-like stem cells from cord blood is mediated by PKABiochemical and biophysical research communications17466951Cell culture and growth profiling. MNCs (AllCells) were seeded in MyeloCult medium (Stem Cell Technologies) supplemented with 1 × 10−7 M dexamethasone (Sigma). Adherent cells were trypsinized (0.125% Trypsin-EDTA) and passaged at near-confluency (70-Full Paper
2007Chen, L;Gao, Z;Zhu, J;Rodgers, GP;Identification of CD13+CD36+ cells as a common progenitor for erythroid and myeloid lineages in human bone marrowExperimental hematology17588473Human bone marrow CD133+ cells (AllCells, Berkeley, CA, USA) were cultured for 14-28 days in the presence of EPO or G-CSF. All cells were grown in media that included stem cell factor (SCF, 50 ng/mL), interleukin-3 (IL-3, 10 ng/mL), granulocyte-macroFull Paper
2007Chua, KN;Chai, C;Lee, PC;Ramakrishna, S;Leong, KW;Mao, HQ;Functional nanofiber scaffolds with different spacers modulate adhesion and expansion of cryopreserved umbilical cord blood hematopoietic stem/progenitor cellsExperimental hematology17577926Frozen human umbilical cord blood CD34+ HSPCs were purchased from AllCells, LLC (Emeryville, CA, USA). The CD34+ purity in the post-thawed HSPC was determined to be 98% by flow cytometry and the viability was determined to be more than 97% by TrypanFull Paper
2007Zwezdaryk, KJ;Coffelt, SB;Figueroa, YG;Liu, J;Phinney, DG;LaMarca, HL;Florez, L;Morris, CB;Hoyle, GW;Scandurro, AB;Erythropoietin, a hypoxia-regulated factor, elicits a pro-angiogenic program in human mesenchymal stem cellsExperimental hematology17379074Cambrex BioScience (Walkersville, MD, USA) and Allcells (Emeryville, CA, USA), to ensure enough variability of the starting cell population and make certain that findings are universal and not unique to single donor pools derived from a unique sourceFull Paper
2007Cheng, X;Macvittie, T;Meisenberg, B;Welty, E;Farese, A;Tadaki, D;Takebe, N;Human brain endothelial cells (HUBEC) promote SCID repopulating cell expansion through direct contactGrowth factors (Chur, Switzerland)18049950The objective of this study was to re-evaluate the previously published hematopoietic stem cell (HSC) expansion work using human brain endothelial cells (HUBEC). The expansion SaveFull Paper
2007Basiji, DA;Ortyn, WE;Liang, L;Venkatachalam, V;Morrissey, P;Cellular image analysis and imaging by flow cytometryClinics in laboratory medicine17658411To investigate the utility of imaging flow cytometry for clinical FISH analysis, human peripheral blood mononuclear cells (PBMC) were obtained (AllCells, San Diego, CA) and probed using a FISH in suspension (FISHIS®) protocol developed at Amnis CorpoFull Paper
2007King, JA;Cell culture systems inspired by the hematopoietic stem cell nicheThesiswere maintained between 0.5 and 1x106 cells/mL. 3.3.5 Culture of CD34+ selected cells CD34+ selected cells were purchased from AllCells (Berkeley, CA). AllCells obtained informed consent from volunteers before donors provided cellsFull Paper
2007Bell, A;Meyer-Ebrecht, D;Bocking, A;Aach, T;HDR-Microscopy of Cell Specimens: Imaging and Image Analysis2007 Conference Record of the Forty-First Asilomar Conference on Signals, Systems and ComputersThe counterstain affects allcells, ie, independent of the protein which is stained by the immunocytochemical marker. Hence, marker positive cells will be affected by both stains at once. SaveFull Paper
2007이연경, ;Effects of cationic liposomes on in vitro and in vivo transduction mediated by adenoviral vectorsThesisAccording to theresultofGFP expression in HeLa cells(Figure 3A),nearly allcells were transduced in the presence of the 100 MOIof Ad-vectors. Meanwhile, CAR-deficient B16BL6 cells SaveFull Paper
2007NGIAP, CK;Bio-functionalization of Electrospun Nanofibre Scaffolds for Cell Culture ApplicationsThesisFrozen human umbilical cord blood CD34+ HSPCs were purchased from AllCells, which were obtained from normal volunteers participating in an Institute Reviewing Board (IRB) approved donor program (AllCells). The CD34+ purity in the HSPC was determineFull Paper
2007Giammona, LM;Nicotinamide enhances primary human megakaryocytic differentiation from hematopoietic stem cells: Phenotypic characterization and mechanism of actionThesisUnless otherwise noted, all reagents were obtained from Sigma-Aldrich (St. Louis, MO). Cultures were initiated with previously frozen human mobilized peripheral blood CD34+ cells (AllCells; Berkeley, CA). Cells were cultured in T-flasks and maintaineFull Paper
2007Troken, AJ;Mesenchymal stem cells and derivatives in hydrogelsThesisHMSCs were isolated from commercially available healthy human bone marrow (AllCells, LLC; Berkeley, CA) using RosetteSep kit (StemCell Technologies, Inc., Vancouver, BC, Canada). Bone marrow extracts contain heterogeneous cell populations. MSCs repreFull Paper
2006Perk, J;Gil-Bazo, I;Chin, Y;de Candia, P;Chen, JJ;Zhao, Y;Chao, S;Cheong, W;Ke, Y;Al-Ahmadie, H;Gerald, WL;Brogi, E;Benezra, R;Reassessment of id1 protein expression in human mammary, prostate, and bladder cancers using a monospecific rabbit monoclonal anti-id1 antibodyCancer Res.17108123Two slides of commercially available (Folio Biosciences, Columbus, OH) TMAs were also analyzed. The two slides included a total of 143 cores, of which 62 samples were common types of breast cancer, and the rest of the samples were benign (slides #ARYFull Paper
2006Swales, KE;Korbonits, M;Carpenter, R;Walsh, DT;Warner, TD;Bishop-Bailey, D;The farnesoid X receptor is expressed in breast cancer and regulates apoptosis and aromatase expressionCancer Res.17047076Samples of human infiltrating ductal breast carcinoma and near tumor normal samples were obtained from 10 patients after receiving ethical approval and individual patient consent. Protein was extracted using Cytobuster (Novagen, Merck Biosciences, NoFull Paper
2006Evans, EE;Henn, AD;Jonason, A;Paris, MJ;Schiffhauer, LM;Borrello, MA;Smith, ES;Sahasrabudhe, DM;Zauderer, M;C35 (C17orf37) is a novel tumor biomarker abundantly expressed in breast cancerMol. Cancer Ther.17121940Staining of formalin-fixed, paraffin-embedded breast carcinoma and normal tissue sections was done in part by QualTek Molecular Labs (Santa Barbara, CA). Ten lobular and 50 invasive ductal (grades 1, 2, and 3) carcinomas were selected from the QualTeFull Paper
2006Roth, JD;Hughes, H;Kendall, E;Baron, AD;Anderson, CM;Antiobesity effects of the beta-cell hormone amylin in diet-induced obese rats: effects on food intake, body weight, composition, energy expenditure, and gene expressionEndocrinology16935845To compare central nervous system gene expression across amylin- and pair-fed-treated rats that had exhibited a similar magnitude of weight loss, four animals from each group were selected for ISH, counterbalanced for their final body weight on the dFull Paper
2006Couse, JF;Yates, MM;Rodriguez, KF;Johnson, JA;Poirier, D;Korach, KS;The intraovarian actions of estrogen receptor-alpha are necessary to repress the formation of morphological and functional Leydig-like cells in the female gonadEndocrinology16627580... }X56974 [/nuccore/X56974] 1271–1351 GAAACTGCGAATGGCTCATTAA GAATTACCACAGTTATCCAAGTAGGA Open in a separate window [/pmc/articles/PMC1892221/table/T1/?report=objectonly] IN SITU HYBRIDIZATION Phylogeny Inc. (Columbus, OH) conducted all _inFull Paper
2006Costello, C;Ward, M;Search, bioprospecting and biodiversity conservationJournal of Environmental Economics and Managementin a particular way. When executed in bulk, these tests cost between $4000 and $18,000 per species tested (eg Qualtek Molecular Laboratories, Santa Barbara, CA). We use a range for c of $4000-$18,000. • Discount rate (rFull Paper
2006Cantwell, CP;Lynch, FC;Ureterocutaneous fistula and urostomy exclusion with use of a covered wallstentJ Vasc Interv Radiol16614159Full Paper
2006Kutty, RK;Chen, S;Samuel, W;Vijayasarathy, C;Duncan, T;Tsai, JY;Fariss, RN;Carper, D;Jaworski, C;Wiggert, B;Cell density-dependent nuclear/cytoplasmic localization of NORPEG (RAI14) proteinBiochem. Biophys. Res. Commun.16729964Immunohistochemistry. Immunohistochemistry was performed by QualTek Molecular Laboratories. Briefly, four-micron human placenta sections were deparaffinized, subjected to 20 min of steam heat induced epitope recovery, and then treated with anti-NORPEFull Paper
2006Cantwell, C;Waybill, P;Lynch, F;Taubman, K;Inferior vena cava duplication and deep venous thrombosisAnn Vasc Surg16575497Full Paper
2006Huang, LT;Transcriptional analysis of ex vivo granulocyte (neutrophil) developmentThesisCultures were initiated with fresh or frozen G-CSF mobilized peripheral blood CD34+ cells from normal donors (AllCells, Emeryville, CA), at a seeding density of 3 x 104 cells/mL. Cells were cultured in human long-term medium (HLTM) at a pH of 7.1 anFull Paper
2006Chen, C;Huang, X;Atakilit, A;Zhu, QS;Corey, SJ;Sheppard, D;The Integrin alpha9beta1 contributes to granulopoiesis by enhancing granulocyte colony-stimulating factor receptor signalingImmunity17137800Murine bone marrow or peripheral blood cells were collected at autopsy and suspended in phosphate-buffered saline (PBS). Human bone marrow cells were obtained from Allcells (Berkeley, CA). Cells were incubated with primary antibody for 30 min at 4°C,Full Paper
2006Guerzoni, C;Bardini, M;Mariani, SA;Ferrari-Amorotti, G;Neviani, P;Panno, ML;Zhang, Y;Martinez, R;Perrotti, D;Calabretta, B;Inducible activation of CEBPB, a gene negatively regulated by BCR/ABL, inhibits proliferation and promotes differentiation of BCR/ABL-expressing cellsBlood16418324CD34+ normal bone marrow (NBM) cells from different healthy donors were purchased from AllCells (LLC, Berkley, CA) and their use was approved by the Ohio State University Institutional Review Board (IRB). Mononuclear hematopoietic cells from bone marFull Paper
2006Notari, M;Neviani, P;Santhanam, R;Blaser, BW;Chang, JS;Galietta, A;Willis, AE;Roy, DC;Caligiuri, MA;Marcucci, G;Perrotti, D;A MAPK/HNRPK pathway controls BCR/ABL oncogenic potential by regulating MYC mRNA translationBlood16293596CD34+ normal bone marrow (NBM) cells from healthy donors were from AllCells (Berkeley, CA). Studies performed with human specimens from The Ohio State University Leukemia Tissue Bank (Columbus, OH), the Division of Hematology, Maisonneuve-Rosemont HoFull Paper
2006Choi, J;Garewal, H;Riley, C;Cooke, L;Klinkhammer, T;Maddipati, S;Sud, R;Spier, C;Mahadevan, D;Transcriptosome and Serum Cytokine Profiling of B-CLL Identifies Oncogenic Pathways in Pathogenesis and Survival.BloodFull Paper
2006Greaney, P;Nahimana, A;Lagopoulos, L;Etter, AL;Aubry, D;Attinger, A;Beltraminelli, N;Huni, B;Bassi, I;Sordat, B;Demotz, S;Dupuis, M;Duchosal, MA;A Fas agonist induces high levels of apoptosis in haematological malignanciesLeukemia research16181674CLL 1, 2 and 3, κ+). These cells were frozen in medium containing 10% DMSO within 10 h after harvesting. Haematopoietic progenitor cells from cord blood were obtained frozen from AllCells (San Mateo, CA)Full Paper
2006Chua, KN;Chai, C;Lee, PC;Tang, YN;Ramakrishna, S;Leong, KW;Mao, HQ;Surface-aminated electrospun nanofibers enhance adhesion and expansion of human umbilical cord blood hematopoietic stem/progenitor cellsBiomaterials16854459Interaction between hematopoietic stem/progenitor cells (HSPCs) and their extra cellular matrix components is an integral part of the signaling control for HSPC survival, proliferation SaveFull Paper
2006La Rosée, P;Jia, T;Demehri, S;Härtel, N;de Vries, P;Bonham, L;Hollenback, D;Singer, JW;Melo, JV;Druker, BJ;Deininger, MW;Antileukemic activity of lysophosphatidic acid acyltransferase-beta inhibitor CT32228 in chronic myelogenous leukemia sensitive and resistant to imatinibClinical cancer research : an official journal of the American Association for Cancer Research17085669without erythropoietin [for culture of granulocyte-macrophage colony-forming unit (CFU-GM)] were done as described previously (26). Normal bone marrow was purchased commercially (AllCells, LLC, Berkeley, CA). Patient samples were collected from patieFull Paper
2006Yang, L;Bailey, L;Baltimore, D;Wang, P;Targeting lentiviral vectors to specific cell types in vivoProceedings of the National Academy of Sciences of the United States of America16864770106) (AllCells) were incubated with concentrated virus with total TUs of 10 106 fresh human PBMCs (AllCells) were transferred by tail vein injection into each mouse. After 6 h, SaveFull Paper
2006Giammona, LM;Fuhrken, PG;Papoutsakis, ET;Miller, WM;Nicotinamide (vitamin B3) increases the polyploidisation and proplatelet formation of cultured primary human megakaryocytesBritish journal of haematology17054670Unless otherwise noted, all reagents were obtained from Sigma-Aldrich (St Louis, MO, USA). Cultures were initiated with previously frozen human mobilised peripheral blood (mPB) CD34+ cells (AllCells, Berkeley, CA, USA). Cells were cultured in T-flaskFull Paper
2006Wang, HJ;Yu, CL;Kishi, H;Motoki, K;Mao, ZB;Muraguchi, A;Suppression of experimental osteoarthritis by adenovirus-mediated double gene transferChinese medical journal16934183Allcells were grown to approxim ately 60% 一 70% confluence at the third passage before adenoviraI vector transduction experim ents started.The cells were washed with sterile saline SaveFull Paper
2006Cabana, R;Frolova, EG;Kapoor, V;Thomas, RA;Krishan, A;Telford, WG;The minimal instrumentation requirements for Hoechst side population analysis: stem cell analysis on low-cost flow cytometry platformsStem cells (Dayton, Ohio)16888279Institute Laboratory Animal Facility. Mice were euthanized by gradual CO2 exposure according to NIH guidelines. Bone marrow was extracted from femurs and tibias and used with no further fractionation. Frozen human bone marrow and cord blood were obtaFull Paper
2006Yu, X;Alder, JK;Chun, JH;Friedman, AD;Heimfeld, S;Cheng, L;Civin, CI;HES1 inhibits cycling of hematopoietic progenitor cells via DNA bindingStem cells (Dayton, Ohio)16513761Normal human placental/umbilical cord blood (CB) CD34+ cells were purchased from AllCells (Berkeley, CA, http://www.allcells.com). Normal BM and granulocyte-colony stimulating factor (G-CSF)-mobilized peripheral blood stem cell (PBSC) CD34+ cells werFull Paper
2006McIntosh, K;Zvonic, S;Garrett, S;Mitchell, JB;Floyd, ZE;Hammill, L;Kloster, A;Di Halvorsen, Y;Ting, JP;Storms, RW;Goh, B;Kilroy, G;Wu, X;Gimble, JM;The immunogenicity of human adipose-derived cells: temporal changes in vitroStem cells (Dayton, Ohio)16410391Human bone marrow was purchased from Cambrex (Walkersville, MD, http://www.cambrex.com) or AllCells, LLC (Berkeley, CA). Bone marrow aspirates were collected with heparin and fractionated over a 1.073 g/ml density gradient (Lymphocyte Separation MediFull Paper
2006Miura, M;Miura, Y;Padilla-Nash, HM;Molinolo, AA;Fu, B;Patel, V;Seo, BM;Sonoyama, W;Zheng, JJ;Baker, CC;Chen, W;Ried, T;Shi, S;Accumulated chromosomal instability in murine bone marrow mesenchymal stem cells leads to malignant transformationStem cells (Dayton, Ohio)16282438Human bone marrow aspirates from healthy adult volunteers were purchased from AllCells, LLC (Berkeley, CA, http://www.allcells.com). To identify putative BMMSCs, single-cell suspension of 1 × 106 of bone marrow mononuclear cells was seeded into 15-cmFull Paper
2006Muramoto, GG;Chen, B;Cui, X;Chao, NJ;Chute, JP;Vascular endothelial cells produce soluble factors that mediate the recovery of human hematopoietic stem cells after radiation injuryBiology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation16635788Cryopreserved human BM CD34+ (Cambrex, Gaithersburg, MD) or CB CD34+ cells (AllCells, Berkeley, CA) were thawed, washed once, and resuspended at 1 × 106/mL in Iscove modified Dulbecco medium (IMDM; Invitrogen) containing 10% FBS and 1% penicillin/strFull Paper
2006Wang, J;Wu, X;Simonavicius, N;Tian, H;Ling, L;Medium-chain fatty acids as ligands for orphan G protein-coupled receptor GPR84The Journal of biological chemistry16966319Quantitative Reverse Transcriptase-mediated PCR (Q-RT-PCR) Analysis—Total RNA from human or mouse tissues (Clontech) and human immune cells (AllCells, LLC) were treated with DNase I (Ambion) before reverse transcription. Q-RT-PCR was performed on anFull Paper
2006Wang, J;Simonavicius, N;Wu, X;Swaminath, G;Reagan, J;Tian, H;Ling, L;Kynurenic acid as a ligand for orphan G protein-coupled receptor GPR35The Journal of biological chemistry16754668Cytokine Secretion—Human peripheral blood mononuclear cells and CD14 + monocytes were purchased from Allcells. Peripheral blood mononuclear cells were seeded at a density of SaveFull Paper
2006Slukvin, II;Vodyanik, MA;Thomson, JA;Gumenyuk, ME;Choi, KD;Directed differentiation of human embryonic stem cells into functional dendritic cells through the myeloid pathwayJournal of immunology (Baltimore, Md. : 1950)16493050Adult PBMC were purchased from AllCells. Monocytes were isolated by adherence to plastic and were cultured for 6 days in RPMI 1640 (Invitrogen Life Technologies) containing 1% FBS (HyClone) in the presence of 100 ng/ml GM-CSF and 100 ng/ml IL-4 withFull Paper
2006Zhang, W;Hirschler-Laszkiewicz, I;Tong, Q;Conrad, K;Sun, SC;Penn, L;Barber, DL;Stahl, R;Carey, DJ;Cheung, JY;Miller, BA;TRPM2 is an ion channel that modulates hematopoietic cell death through activation of caspases and PARP cleavageAmerican journal of physiology. Cell physiology16306129RNA from CD34 + cells and fresh monocytes was purchased from AllCells (Foster City, CA). cDNA was prepared from RNA with the Superscript first-strand synthesis system (Invitrogen, SaveFull Paper
2006Geddis, AE;Kaushansky, K;Endomitotic megakaryocytes form a midzone in anaphase but have a deficiency in cleavage furrow formationCell cycle (Georgetown, Tex.)16552179albumin gradient. For human MKs, CD34+ cells were purchased from AllCells (Berkley, CA) and thawed according to manufacturer’s instructions. The cells were then differentiated by culture in StemPro (Invitrogen) containing 100 ng/ml human TPO, 10 ng/mFull Paper
2006Shen, Y;Chow, J;Wang, Z;Fan, G;Abnormal CpG island methylation occurs during in vitro differentiation of human embryonic stem cellsHuman molecular genetics16870691Medium was changed everyday for optimal cell growth. Fetal astrocytes were purchased from AllCells LLC., and cultures were expanded as suggested by the vendor. Fetal brain tissue was obtained from the Surgical Pathology Laboratory at UCLA (Dr. MichaeFull Paper
2006Polson, AG;Zheng, B;Elkins, K;Chang, W;Du, C;Dowd, P;Yen, L;Tan, C;Hongo, JA;Koeppen, H;Ebens, A;Expression pattern of the human FcRH/IRTA receptors in normal tissue and in B-chronic lymphocytic leukemiaInternational immunology16849395Human blood samples were collected from healthy individuals through the Genentech research blood program; bone marrow was obtained from AllCells (Berkeley, CA, USA) and SaveFull Paper
2006Zhang, X;Kim, J;Ruthazer, R;McDevitt, MA;Wazer, DE;Paulson, KE;Yee, AS;The HBP1 transcriptional repressor participates in RAS-induced premature senescenceMolecular and cellular biology16966377Oncogene-mediated premature senescence has emerged as a potential tumor-suppressive mechanism in early cancer transitions. Previous work shows that RAS and p38 MAPK SaveFull Paper
2006Feng, Q;Chai, C;Jiang, XS;Leong, KW;Mao, HQ;Expansion of engrafting human hematopoietic stem/progenitor cells in three-dimensional scaffolds with surface-immobilized fibronectinJournal of biomedical materials research. Part A16739181Frozen human CB CD34+ cells were purchased from All-Cells (San Mateo, CA), which were obtained from normal volunteers participating in an Institute Reviewing Board (IRB) approved donor program (AllCells). Cells (92% CD34+, 95% viable) were thawed accFull Paper
2006Weigel-Kelley, KA;Yoder, MC;Chen, L;Srivastava, A;Role of integrin cross-regulation in parvovirus B19 targetingHuman gene therapy16972759Primary human CD71 erythroid progenitor cells were purchased from AllCells (Berkeley, CA) and received in Iscove's modified Dulbecco's medium (IMDM)- 0.5% bovine serum SaveFull Paper
2006Braselmann, S;Taylor, V;Zhao, H;Wang, S;Sylvain, C;Baluom, M;Qu, K;Herlaar, E;Lau, A;Young, C;Wong, BR;Lovell, S;Sun, T;Park, G;Argade, A;Jurcevic, S;Pine, P;Singh, R;Grossbard, EB;Payan, DG;Masuda, ES;R406, an orally available spleen tyrosine kinase inhibitor blocks fc receptor signaling and reduces immune complex-mediated inflammationThe Journal of pharmacology and experimental therapeutics16946104Stimulation of Macrophages Derived from Primary Monocytes with IgG or LPS. Human primary macrophages were derived from CD14+ peripheral blood mononuclear cell (AllCells, LLC, Emeryville, CA) according to the protocol specified in the monocyte isolatiFull Paper
2006Xu, H;Othman, SF;Hong, L;Peptan, IA;Magin, RL;Magnetic resonance microscopy for monitoring osteogenesis in tissue-engineered construct in vitroPhysics in medicine and biology16424591Mesenchymal stem cells (MSCs) isolated from fresh adult human bone marrow were provided commercially by AllCells (AllCells LLC, Berkeley, CA). Nucleated cells were incubated in SaveFull Paper
2006Troken, A;Marion, N;Mao, J;HETEROTYPIC ADIPOGENIC PHENOTYPE WHEN EMPLOYING CHONDROGENIC TREATMENT OF HUMAN MESENCHYMAL STEM CELLSASAIO JournalDonated hMSCs (AllCells, LLC) were isolated and expanded using basal media. Upon 90% confluence, the cultures were plated at a density of 24,000 cells/cm 2 in 6-well plates. Three SaveFull Paper
2006Yamada, H;Kunisato, A;Kawahara, M;Tahimic, CGT;Ren, X;Ueda, H;Nagamune, T;Katoh, M;Inoue, T;Nishikawa, M;Oshimura, M;Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosomeJournal of human genetics16333524Both EGFP-21ΔqHAC vectors and ScFvg-21ΔqHAC vectors retained in CHO cells were transferred to 1×107 hBM MNCs (AllCells, LLC, Berkeley, CA, USA) or 1×106 hCB CD34+ cells by microcell-mediated chromosome transfer (MMCT) as previously described (DohertyFull Paper
2006Wang, T;Blatt, LM;Seiwert, SD;Immunomodulatory activities of IFN-gamma1b in combination with type I IFN: implications for the use of IFN-gamma1b in the treatment of chronic HCV infectionsJournal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research16800786IL-4 for 2 weeks), and immature pDCs (IFN-producing cells [IPC], defined as CD11C, CD123, and Lin) were purchased from AllCells, LLC (Berkeley, CA). The procedures for their collection were approved by an Institute Review Board and complied with locaFull Paper
2006Duramad, P;Tager, IB;Leikauf, J;Eskenazi, B;Holland, NT;Expression of Th1/Th2 cytokines in human blood after in vitro treatment with chlorpyrifos, and its metabolites, in combination with endotoxin LPS and allergen Der p1Journal of applied toxicology : JAT16871525emale) who were sampled repeatedly, for experiments1-13, or were purchased (n=8) from AllCells (Berkeley,CA) for experiments 14 -19. Specimens were collecteddirectly into vacutainers with heparin and processedwithin 2Full Paper
2006Aslan, H;Zilberman, Y;Arbeli, V;Sheyn, D;Matan, Y;Liebergall, M;Li, JZ;Helm, GA;Gazit, D;Gazit, Z;Nucleofection-based ex vivo nonviral gene delivery to human stem cells as a platform for tissue regenerationTissue engineering16674300Human mesenchymal stem cells (hMSCs) were isolated from the bone marrow of an anonymous healthy donor (AllCells, Berkeley, CA), cultureexpanded and seeded in 6-well plates at a density of 30,000 cells/well. ForFull Paper
2006Peptan, IA;Hong, L;Xu, H;Magin, RL;MR assessment of osteogenic differentiation in tissue-engineered constructsTissue engineering16674297Human bone marrow cells provided commercially(AllCells Limited Liability Company, Berkeley, CA)were plated at a density of 107nucleated cells per 100mm petri dish and incubated in a culture medium com-posed of Dulbecco’s modifiedFull Paper
2006Stroke, IL;Cole, AG;Simhadri, S;Brescia, MR;Desai, M;Zhang, JJ;Merritt, JR;Appell, KC;Henderson, I;Webb, ML;Identification of CXCR3 receptor agonists in combinatorial small-molecule librariesBiochemical and biophysical research communications16930533Human peripheral blood mononuclear cells (AllCells, Berkeley, CA) were cultured for 3 days with 10 μg/mL phytohemagglutinin, then for 4-7 days with 100 U/mL interleukin-2 ( SaveFull Paper
2006Sugo, T;Tachimoto, H;Chikatsu, T;Murakami, Y;Kikukawa, Y;Sato, S;Kikuchi, K;Nagi, T;Harada, M;Ogi, K;Ebisawa, M;Mori, M;Identification of a lysophosphatidylserine receptor on mast cellsBiochemical and biophysical research communications16460680The total RNAs from human basophils and eosinophils were purchased from AllCells Limited Liability Company (Berkeley, CA). Total RNA was reverse transcribed using Superscript SaveFull Paper
2006Ong, HT;Timm, MM;Greipp, PR;Witzig, TE;Dispenzieri, A;Russell, SJ;Peng, KW;Oncolytic measles virus targets high CD46 expression on multiple myeloma cellsExperimental hematology16728275Fresh bone marrow aspirate from a healthy donor was purchased from AllCells (Berkeley, CA, USA). The mononuclear cell fraction was isolated by Ficoll-Hypaque gradient and used in SaveFull Paper
2006Yoshikubo, T;Inoue, T;Noguchi, M;Okabe, H;Differentiation and maintenance of mast cells from CD34(+) human cord blood cellsExperimental hematology16543066Frozen human umbilical cord blood CD34+ cells were purchased from AllCells, LLC (Berkeley, CA, USA). The mouse bone marrow-derived stroma cell line MS-5 was kindly provided by K. Itoh [23]. MS-5 cells were maintained in a minimal essential medium α (Full Paper
2006Hong, L;Peptan, IA;Xu, H;Magin, RL;Nondestructive evaluation of osteogenic differentiation in tissue-engineered constructsJournal of orthopaedic research : official publication of the Orthopaedic Research Society16583444Healthy fresh human bone marrow was provided commercially (AllCells Limited Liability Company, Emeryville, CA) and plated at a density of 107 nucleated cells per 100 mm petri dish. The cells were incubated in a basic medium of Dulbecco’s Modified EagFull Paper
2006Cole, AG;Stroke, IL;Brescia, MR;Simhadri, S;Zhang, JJ;Hussain, Z;Snider, M;Haskell, C;Ribeiro, S;Appell, KC;Henderson, I;Webb, ML;Identification and initial evaluation of 4-N-aryl-[1,4]diazepane ureas as potent CXCR3 antagonistsBioorganic & medicinal chemistry letters16213722一般社団法人情 処理学会では複写複製および転載複製に係る著作権を学術著作権協会に委託 しています. 当該利用をご希望の方は, 学術著作権協会 が提供している複製利用許諾システ SaveFull Paper
2006Liu, WM;Li, R;Sun, JZ;Wang, J;Tsai, J;Wen, W;Kohlmann, A;Williams, PM;PQN and DQN: algorithms for expression microarraysJournal of theoretical biology16889801Here, we disclose two triplets of experiments using 3 ml fresh bone marrow from a healthy donor obtained from AllCells, LLC (Berkeley, CA). The sample was stored at for 48 h. Then total RNA was purified from the sample with RNeasy mini kit (Qiagen,Full Paper
2006Doshi, M;Koyanagi, M;Nakahara, M;Saeki, K;Saeki, K;Yuo, A;Identification of human neutrophils during experimentally induced inflammation in mice with transplanted CD34+ cells from human umbilical cord bloodInternational journal of hematology17050197MBR1520-3 x-ray source (Hitachi Medical, Tokyo, Japan). After 24 hours, mice received intravenous transplants of 1.8 × 105 human CB CD34+ cells (AllCells, Berkeley, CA, USA) or vehicle (saline). The purity and viability of the CB CD34+ cells were greFull Paper
2006Marion, N;Mao, J;Mesenchymal Stem Cells and Tissue EngineeringMethods in EnzymologyHuman Bone Marrow-Derived MSCs • Ficoll‐Paque—room temperature (e.g., StemCells, Inc., Vancouver, BC, Canada). • Bone marrow sample—room temperature (e.g., AllCells, LLC, Berkeley, CA). • 10 ml marrow + 5 ml DPBS + 125 U/ml heparin (total volume, 1Full Paper
2006Hawley, R;Ramezani, A;Hawley, T;Hematopoietic Stem CellsAdult Stem Cellsperipheral blood cells after informed consent in conformity with a human subjects protocol approved by an institutional review board, or purchase from a commercial source [eg, AllCells SaveFull Paper
2006Nudera, J;Mechanical stresses elicit osteogenic changes in HMSCS encapsulated in a polyethylene glycol scaffoldThesisThe samples of fresh bone marrow (Allcells, Berkeley, CA) were delivered in 10ml amoimts. Both of the patients tested negative for human immunodeficiency virus (HIV), hepatitis B SaveFull Paper
2006Crot, CA;Probing the solid-liquid interface of synthetic biomaterials via x-ray scattering techniquesThesisThe general biocompatibility of the allylamine films is compared with that of the native oxide surface of Ti by culturing human mesenchymal stems cells (hMSCs) isolated from healthy donors (AllCells, Berkeley, CA) (82). HMSCs are plated in monolayerFull Paper
2006Lee, CH;Moioli, EK;Mao, JJ;Fibroblastic differentiation of human mesenchymal stem cells using connective tissue growth factorConference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference17946857Human bone marrow samples taken from healthy donors ranging from 18 to 45 years of age were purchased from AllCells (Berkeley, CA). The bone marrows were prepared to isolate hMSCs per our previous methods [10], [11]. Briefly, the whole bone marrow saFull Paper
2006Moinnes, JJ;Vidula, N;Halim, N;Othman, SF;Ultrasound accelerated bone tissue engineering monitored with magnetic resonance microscopyConference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference17946836Mesenchymal stem cells (MSCs) isolated from fresh adult human bone marrow were provided by AllCells (AllCells LLC, Berkeley, CA). Nucleated cells were expanded via incubation SaveFull Paper
2006Dravid, G;Hammond, H;Cheng, L;Culture of human embryonic stem cells on human and mouse feeder cellsMethods in molecular biology (Clifton, N.J.)16881511mitomycin C-treated (Speciality Media; cat. no. PMEF-CF [mitomycin-treated]) (see Note 5 ). 3. Normal breast feeder cells: CCD-1087sk (American Type Culture Collection, Manassas, VA; cat. no. CRL-2104). 4. Bone marrow mononuclear cells (AllCells; LFull Paper
2006MacDonald, M;The DataGridViewPro .NET 2.0 Windows Forms and Custom Controls in C#Similar to AllCells, except the DataGridView only considers the rows that are currently visible at the time the property is set. This option is used to improve performance for large grids. SaveFull Paper
2006Crossman, LC;Predictors of imatinib response in patients with chronic myeloid leukaemiaThesisallowing us to compare the groups pre-imatinib. All patients consented to enter this study (Appendix 2). Four samples of healthy BM MNC's were obtained from AllCells (Berkeley, CA), to act as normal controlsFull Paper
2006Chen, C;Megakaryocytic* transcriptional program and developmental plasticityThesisMk and G cell cultures were initiated with fresh G-CSF-mobilized human peripheral blood CD34+ cells from normal donors (AllCells; Berkeley, CA). For Mk cultures, cells were cultured in X-VIVO 20 serum-free medium (BioWhittaker; Walkersville, MD) supFull Paper
2006Yourek, G;Effects of differentiation and shears stress on stem cell gene expression and nanomechanicsThesisWhole bone marrow (AllCells, Berkeley, CA) was mixed with "RosetteSep®" (StemCell Technologies; Vancouver, BC) (50 pL per 1 mL of bone marrow) and incubated for 20 minutes at room temperature (RT). The "RosetteSep®" antibody cocktail crosslinks unwanFull Paper
2006Clark, PA;Modulating bone ingrowth into titanium implants by growth factor delivery and mechanical loadingThesisHuman mesenchymal stem cell isolation and expansion Human mesenchymal stem cells (hMSCs) were isolated from bone marrow. Whole human bone marrow was purchased commercially (AllCells LLC, Berkeley, CA). Negative selection (RosetteSep, StemCellFull Paper
2006Charoonpatrapong, K;The roles of nuclear matrix protein 4 and high mobility group box 1 in mediating the osteoblast response to anabolic agentsThesisHuman cord blood-derived osteoclastogenesis was performed as described previously (Fuson & Galvin, 2002) with slight modification. Frozen human cord blood mononuclear cells (CBMNCs) were purchased from AllCells (Berkeley, CA). CBMNCs were quickly thaFull Paper
2005De Vry, CG;Valdez, M;Lazarov, M;Muhr, E;Buelow, R;Fong, T;Iyer, S;Topical application of a novel immunomodulatory peptide, RDP58, reduces skin inflammation in the phorbol ester-induced dermatitis modelJ. Invest. Dermatol.1611778810% neutral-buffered formalin (Fisher Scientific). The preparation of tissues by embedding in paraffin, sectioning, and staining was carried out by QualTek Molecular Laboratories (Santa Barbara, California). A series of ear crossFull Paper
2005Finnberg, N;Gruber, JJ;Fei, P;Rudolph, D;Bric, A;Kim, SH;Burns, TF;Ajuha, H;Page, R;Wu, GS;Chen, Y;McKenna, WG;Bernhard, E;Lowe, S;Mak, T;El-Deiry, WS;DR5 knockout mice are compromised in radiation-induced apoptosisMol. Cell. Biol.15713653... ts separated on a 2% agarose gel. Mean band density was determined with NIH Image 1.62 and normalized to the band density of 18S rRNA. IN SITU HYBRIDIZATION. In situ hybridization was performed by Phylogeny Inc. as described (17). Briefly, fetFull Paper
2005Schirmer, A;Gadkari, R;Reeves, CD;Ibrahim, F;DeLong, EF;Hutchinson, CR;Metagenomic analysis reveals diverse polyketide synthase gene clusters in microorganisms associated with the marine sponge Discodermia dissolutaAppl. Environ. Microbiol.16085882We thank Dan Morse (University of California, Santa Barbara) and David Myles for obtaining Discodermia dissoluta, Steve Bernstein (QualTek Molecular Laboratories, Santa Barbara, CA) for help with microscopy on sponge thin sections, and Ralph Reid forFull Paper
2005Pyle-Chenault, RA;Stolk, JA;Molesh, DA;Boyle-Harlan, D;McNeill, PD;Repasky, EA;Jiang, Z;Fanger, GR;Xu, J;VSGP/F-spondin: a new ovarian cancer markerTumour Biol.16103746Rockford, Ill., USA). Immunohistochemistry was performed with affinity-purified rabbit polyclonal antibody on formalin-fixed, paraffin-embedded tissues by QualTek Molecular Laboratories (Santa Barbara, Calif., USA). Steam heatFull Paper
2005Wang, B;Berger, M;Masters, G;Albone, E;Yang, Q;Sheedy, J;Kirksey, Y;Grimm, L;Wang, B;Singleton, J;Soltis, D;Radiotherapy of human xenograft NSCLC tumors in nude mice with a 90Y-labeled anti-tissue factor antibodyCancer Biother. Radiopharm.15989475An IHC study of TF expression in human tissues was performed at QualTek Labs (Newton, PA). Briefly, formalin-fixed, paraffin-embedded sec- tions from human NSCLC tumors and different normal human tissues were taken from QualTek's human tissue bankFull Paper
2005Urnov, FD;Miller, JC;Lee, YL;Beausejour, CM;Rock, JM;Augustus, S;Jamieson, AC;Porteus, MH;Gregory, PD;Holmes, MC;Highly efficient endogenous human gene correction using designed zinc-finger nucleasesNature15806097Human CD4+ T cells (AllCells) were grown according to the supplier's instructions. Cells were activated using 20 Units per ml IL-2 (R&D Systems) and αCD3/CD28 beads (Miltenyi Biotec, T Cell Activation/Expansion kit), and transfected with ZFN and donoFull Paper
2005Freud, AG;Becknell, B;Roychowdhury, S;Mao, HC;Ferketich, AK;Nuovo, GJ;Hughes, TL;Marburger, TB;Sung, J;Baiocchi, RA;Guimond, M;Caligiuri, MA;A human CD34(+) subset resides in lymph nodes and differentiates into CD56bright natural killer cellsImmunity15780987Fresh human BM was donated or purchased from AllCells, LLC (Berkeley, CA) and was received within 24 hr of harvest. PB leukopaks were obtained from the American Red Cross. BM SaveFull Paper
2005Neviani, P;Santhanam, R;Trotta, R;Notari, M;Blaser, BW;Liu, S;Mao, H;Chang, JS;Galietta, A;Uttam, A;Roy, DC;Valtieri, M;Bruner-Klisovic, R;Caligiuri, MA;Bloomfield, CD;Marcucci, G;Perrotti, D;The tumor suppressor PP2A is functionally inactivated in blast crisis CML through the inhibitory activity of the BCR/ABL-regulated SET proteinCancer cell16286244The oncogenic BCR/ABL kinase activity induces and maintains chronic myelogenous leukemia (CML). We show here that, in BCR/ABL-transformed cells and CML blast crisis (CML-BC) SaveFull Paper
2005Crossman, LC;Druker, BJ;Deininger, MW;Pirmohamed, M;Wang, L;Clark, RE;hOCT 1 and resistance to imatinibBlood16033955mononuclear cells (MNCs) cryopreserved immediately prior to imatinib therapy, and all NRs had BM MNCs stored after 9 to 15 months of imatinib treatment. Four normal BM MNC samples were obtained from AllCells (Berkeley, CA)Full Paper
2005Corbin, AS;Demehri, S;Griswold, IJ;Wang, Y;Metcalf, CA;Sundaramoorthi, R;Shakespeare, WC;Snodgrass, J;Wardwell, S;Dalgarno, D;Iuliucci, J;Sawyer, TK;Heinrich, MC;Druker, BJ;Deininger, MW;In vitro and in vivo activity of ATP-based kinase inhibitors AP23464 and AP23848 against activation-loop mutants of KitBlood15746079Mononuclear cells from 4 independent commercially available normal bone marrow samples (AllCells, Berkeley, CA) were cultured in duplicate plates with doses of AP23464 ranging Save;Hematopoietic colony forming assays: Mononuclear cells from four inFull Paper
2005Orita, T;Tsunoda, H;Yabuta, N;Nakano, K;Yoshino, T;Hirata, Y;Ohtomo, T;Nezu, J;Sakumoto, H;Ono, K;Saito, M;Kumagai, E;Nanami, M;Kaneko, A;Yoshikubo, T;Tsuchiya, M;A novel therapeutic approach for thrombocytopenia by minibody agonist of the thrombopoietin receptorBlood15374889CD34-positive cells (AllCells, LLC, Berkeley, CA) derived from human bone marrow were cultured with rhTPO or VB22B sc(Fv)2 in the presence of 0.2 ng/mL human IL-3 (PeproTech, London, UK) for 11 days at 37°C in Iscove's Modified Dulbecco's Medium (MDMFull Paper
2005Choi, J;Simons, B;Riley, C;Klinkhammer, T;Cooke, L;Spier, C;Sud, R;Hehn, S;Garewal, H;Mahadevan, D;Transcriptosome Profiling of B-CLL Identifies WNT-3A and ROR-1 as an Autocrine Mechanism in Cell Survival.BloodFull Paper
2005Clark, RA;Yamanaka, K;Bai, M;Dowgiert, R;Kupper, TS;Human skin cells support thymus-independent T cell developmentThe Journal of clinical investigation16224538For IFN-γ treated matrices, 50 ng/ml IFN-γ (R&D Systems) was included in the culture medium from plating until day 6. On day 7, 10,000 normal human bone marrow AC133+ precursor cells (AllCells) were added to each matrix, and the unit was cultured inFull Paper
2005Pan, F;Means, AR;Liu, JO;Calmodulin-dependent protein kinase IV regulates nuclear export of Cabin1 during T-cell activationThe EMBO journal15902271Human peripheral blood mononuclear cells (PMBCs) were obtained from AllCells (Berkeley, CA). Naïve CD4+ T cells were purified by depletion of memory T cells as well as non-CD4+ cells, followed by positive selection with magnetic activated cell separaFull Paper
2005O'Hare, T;Walters, DK;Stoffregen, EP;Sherbenou, DW;Heinrich, MC;Deininger, MW;Druker, BJ;Combined Abl inhibitor therapy for minimizing drug resistance in chronic myeloid leukemia: Src/Abl inhibitors are compatible with imatinibClinical cancer research : an official journal of the American Association for Cancer Research16203792Samples were obtained with informed consent from four CML patients or from a tissue repository (AllCells, Berkeley, CA). The CML samples were screened for kinase domain mutations SaveFull Paper
2005Kikuchi, J;Ozaki, H;Nonomura, C;Shinohara, H;Iguchi, S;Nojiri, H;Hamada, H;Kiuchi, A;Nakamura, M;Transfection of antisense core 2 beta1,6-N-acetylglucosaminyltransferase-1 cDNA suppresses selectin ligand expression and tissue infiltration of B-cell precursor leukemia cellsLeukemia16179912(CB) was drawn from umbilical cords of cesarean section and normal full-term deliveries. Mononuclear cells (MNCs) were enriched by Ficoll density gradients and subjected to analysis. Bone marrow (BM) derived MNCs were purchased from AllCells (BerkeleFull Paper
2005D'Costa, J;Chaudhuri, S;Civin, CI;Friedman, AD;CBFbeta-SMMHC slows proliferation of primary murine and human myeloid progenitorsLeukemia15815715Human cord blood CD34+ cells (AllCells Inc., San Mateo, CA, USA) or cryopreserved human mobilized peripheral blood CD34+ cells from the Johns Hopkins cell bank (from donors who gave informed consent on an IRB-approved protocol) were placed directly iFull Paper
2005Leugers, S;Allison, D;Pronold, B;Rennebeck, G;Van Zant, G;Tario, J;Swartzwelder, F;Donahue, L;Stemline Hematopoietic Stem Cell Expansion Medium, a Serum-Free Medium for the Expansion of CD34+ Hematopoietic Stem Cells and ProgenitorsAnimal Cell Technology Meets GenomicsCell Preparation. For all experiments, cryopreserved, human CD34+ cells were obtained from independent suppliers (Stemgenix; AllCells, LLC) and were handled in a manner consistent with the supplier’s instructions with regard to storage and reconstituFull Paper
2005Wang, R;Arevalo-Herrera, M;Gardner, MJ;Bonelo, A;Carlton, JM;Gomez, A;Vera, O;Soto, L;Vergara, J;Bidwell, SL;Domingo, A;Fraser, CM;Herrera, S;Immune responses to Plasmodium vivax pre-erythrocytic stage antigens in naturally exposed Duffy-negative humans: a potential model for identification of liver-stage antigensEuropean journal of immunology15864779vivax blood-stage parasites, 2 were living in California and their PBMC were purchased from AllCells (AllCells, LLC, Berkeley, CA) and used in this study as controls. All volunteers SaveFull Paper
2005Mahadevan, D;Spier, C;Della Croce, K;Miller, S;George, B;Riley, C;Warner, S;Grogan, TM;Miller, TP;Transcript profiling in peripheral T-cell lymphoma, not otherwise specified, and diffuse large B-cell lymphoma identifies distinct tumor profile signaturesMolecular cancer therapeutics16373702Control RNA was obtained from normal peripheral B cells (AllCells, Berkeley, CA), normal peripheral T cells (AllCells), and a snap-frozen normal lymph node (Department of Pathology, SaveFull Paper
2005Siatskas, C;Underwood, J;Ramezani, A;Hawley, RG;Medin, JA;Specific pharmacological dimerization of KDR in lentivirally transduced human hematopoietic cells activates anti-apoptotic and proliferative mechanismsFASEB journal : official publication of the Federation of American Societies for Experimental Biology16076962CD34 + -selected cord blood cells were purchased from AllCells (Berkeley, CA). Cells were maintained at 37C in a humidified atmosphere of 5% CO 2 . Addition of rh stem cell factor ( SaveFull Paper
2005Rao, PE;Petrone, AL;Ponath, PD;Differentiation and expansion of T cells with regulatory function from human peripheral lymphocytes by stimulation in the presence of TGF-{beta}Journal of immunology (Baltimore, Md. : 1950)15661903For T cell differentiation using human cord blood naive CD4 + T cells, frozen CD4 + CD45RA + cells isolated using magnetic beads were obtained from Allcells. Cells were thawed and SaveFull Paper
2005Casida, JE;Quistad, GB;Serine hydrolase targets of organophosphorus toxicantsChemico-biological interactions16243304Whole human blood from seven subjects and lymphocytes (isolated by aphaeresis using a leukopac filter) from three subjects were obtained from AllCells LLC (Emeryville, CA). SaveFull Paper
2005Patel, S;Mehta-Damani, A;Shu, H;Le Pecq, JB;An analysis of variability in the manufacturing of dexosomes: implications for development of an autologous therapyBiotechnology and bioengineering16037986Peripheral blood mononuclear cells (PBMC) obtained from healthy volunteers consisting of 111 leukopacks were obtained from AllCells Inc. (Berkeley, CA), under a protocol approved SaveFull Paper
2005Hong, L;Peptan, I;Clark, P;Mao, JJ;Ex vivo adipose tissue engineering by human marrow stromal cell seeded gelatin spongeAnnals of biomedical engineering15909657Human bone MSCs were isolated from fresh bone marrow of adult males and provided commercially by AllCells (AllCells Limited Liability Company, CA.). Nucleated cells of the fresh bone marrow were counted under inverted microscope, plated at a densityFull Paper
2005Murmann, AE;Gao, J;Encinosa, M;Gautier, M;Peter, ME;Eils, R;Lichter, P;Rowley, JD;Local gene density predicts the spatial position of genetic loci in the interphase nucleusExperimental cell research16202404Primary human bone marrow cells (CD34+ and CD33+) were purchased from AllCells Inc. (Berkeley, CA). Chinese muntjac fibroblasts, isolated from tissue of a stillbirth Chinese muntjac fawn provided by the University of Bielefeld, Department of Animal BFull Paper
2005Alhadlaq, A;Tang, M;Mao, JJ;Engineered adipose tissue from human mesenchymal stem cells maintains predefined shape and dimension: implications in soft tissue augmentation and reconstructionTissue engineering15869434Human MSCs (hMSCs) were isolated from whole bone marrow aspirates of the iliac crest of a healthy 22- year-old male donor (AllCells, Berkeley, CA). Briefly, cells were enriched by adding RosetteSep mesenchymal enrichment cocktail (StemCell TechnologiFull Paper
2005Marion, N;Liang, W;Liang, W;Reilly, G;Day, D;Rahaman, M;Mao, J;Borate Glass Supports the In Vitro Osteogenic Differentiation of Human Mesenchymal Stem CellsMechanics of Advanced Materials and StructuresBone marrow samples taken from healthy donors ranging from 18 to 45 years of age were purchased from AllCells (Berkeley, CA). Human bone marrow derived mesenchymal stem SaveFull Paper
2005Lesniak, MS;Kelleher, E;Pardoll, D;Cui, Y;Targeted gene therapy to antigen-presenting cells in the central nervous system using hematopoietic stem cellsNeurological research16354542Human G-CSF-mobilized peripheral blood or cord blood CD34z cells were obtained from AllCells (San Mateo, CA). CD34z cells were purified by immunomagnetic selection (Miltenyi SaveFull Paper
2005Ivanov, M;Kraiko, A;Numerical solution of the direct-problem of mixed nozzle flowFluid DynamicsFinally, P_I/i, k-1/2 and P-1/2, k-1/2 are determined from the resulting u and v and the given (constant for allcells) entropy and stagnationenthalpy. The calculations showed that the SaveFull Paper
2005Leung, G;Tang, H;McGuinness, R;Verdonk, E;Michelotti, J;Liu, V;Cellular Dielectric Spectroscopy: A Label-Free Technology for Drug DiscoveryJALA: Journal of the Association for Laboratory AutomationHeLa (CCL-2), and U937 (CRL-1593.2)53 were obtained from ATCC (Manassas, VA). Primary neutrophils were purchased from AllCells, LLC (Berkeley, CA). HeLa cells were grown in minimal essential medium (MEM); U937 cells were grown in RPMI-1640; CHOm1 celFull Paper
2005Owens, BM;Hawley, RG;Spain, LM;Retroviral transduction in fetal thymic organ cultureMethods in molecular medicine15492404samples are also available from several commercial sources, for example, AllCells, (Berkeley, CA) or Cambrex BioScience Walkersville, (Walkersville, MD). The thymocyte progenitor population can be transduced with retroviral constructs using VSV-G-pseFull Paper
2005Varma, C;Quantitative isoforrm profiling & isoform convergenceThesisSamples of human peripheral blood purified B cells, human cord blood purified B cells, and human adult whole bone marrow cells derived from normal (i.e. nondiseased) individuals were purchased from ALLCELLS, LLC, Berkeley, California USA. Each sampleFull Paper
2005Gao, J;Quantitative Analysis of Chromosome/Gene Spatial DistributionExperimental Cell ResearchCD34+ and CD33+ cells were primary hematopoietic stem cells and myeloid cells, respectively, (purchased from AllCells, LLC., CA) isolated from bone marrow with a MACS kit, purity of SaveFull Paper
2005Gilison, D;The role of telomerase expression in normal development and cancerThesisFresh human bone marrow was obtained with informed consent from healthy volunteers (AllCells, and Department of Bone Marrow Transplantation, Stanford University School of Medicine). Fresh human cord blood was obtained with informed consent (DepartmenFull Paper
2005Windgassen, D;Der technischen Fakultaet der Universitaet Erlangen-Nuernberg zur Erlangung des GradesThesisHealthy-donor PBMCs (AllCells, Berkeley, CA) were used to select T-cell subsets (CD4(+) and CD8(+) negative selections, Miltenyi Biotech, BergischGladbach, Germany). Briefly, non-CD4(+), CD8(+), i.e. CD4(+) or CD8(+) T cells, γ/δ T cells, B cells, NKFull Paper
2004Douglas, SA;Naselsky, D;Ao, Z;Disa, J;Herold, CL;Lynch, F;Aiyar, NV;Identification and pharmacological characterization of native, functional human urotensin-II receptors in rhabdomyosarcoma cell linesBr. J. Pharmacol.15210573Search for more papers by this author. Frank Lynch QualTek Molecular Laboratories Inc., Newtown, PA 18940, USA Search for more papers by this author. Frank Lynch QualTek Molecular Laboratories Inc., Newtown, PA 18940, USA ;nch Frank 2 Aiyar NaFull Paper
2004Bikle, DD;Chang, S;Crumrine, D;Elalieh, H;Man, MQ;Choi, EH;Dardenne, O;Xie, Z;Arnaud, RS;Feingold, K;Elias, PM;25 Hydroxyvitamin D 1 alpha-hydroxylase is required for optimal epidermal differentiation and permeability barrier homeostasisJ. Invest. Dermatol.15102089Peroxidase activity was revealed with DAB substrate (QualTek Laboratories, Santa Barbara, California) followed by counterstaining with methyl green or hematoxylin. Omitting the first antibodies resulted in no signal, indicating the specificity of immFull Paper
2004Yu, J;He, S;Friedman, JS;Akimoto, M;Ghosh, D;Mears, AJ;Hicks, D;Swaroop, A;Altered expression of genes of the Bmp/Smad and Wnt/calcium signaling pathways in the cone-only Nrl-/- mouse retina, revealed by gene profiling using custom cDNA microarraysJ. Biol. Chem.15292180The labeled transcripts were subjected to alkali hydrolysis (to give an average size of 70 nucleotides) and then hybridized overnight to 5- to 7-μm serial sections of adult mouse retina using standard protocols (Phylogeny Inc., Columbus, OH)Full Paper
2004Lynch, F;The use of an interactive computerized daily schedule in a busy interventional radiology practice increases efficiencyJ Am Coll Radiol17411739Full Paper
2004Kalos, M;Askaa, J;Hylander, BL;Repasky, EA;Cai, F;Vedvick, T;Reed, SG;Wright, GL;Fanger, GR;Prostein expression is highly restricted to normal and malignant prostate tissuesProstate15176054We thank Patrick Roche at the Mayo Clinic and Frank Lynch at Qualtek for performing immunohistochemistry experiments and Masazumi Matsamura for initial work on the generation of the 10E3-G4-D3 monoclonal antibodyFull Paper
2004Tasheva, ES;Ke, A;Conrad, GW;Analysis of the expression of chondroadherin in mouse ocular and non-ocular tissuesMol. Vis.15332017mmol, Amersham Biosciences Corp., Piscataway, NJ). For the ISH experiments, custom service from Phylogeny Inc. (Co- lumbus, OH) was used as previously described [30]. Antibodies: A polyclonal antibody against mouseFull Paper
2004Tasheva, ES;Ke, A;Deng, Y;Jun, C;Takemoto, LJ;Koester, A;Conrad, GW;Differentially expressed genes in the lens of mimecan-null miceMol. Vis.15215744secondary antibody was reacted with avidin-biotin-peroxidase complex solution (Vector Laboratories Inc., Burlingame, CA), with immunoreactivity developed in 3,3'-diaminobenzidine (DAB). In situ hybridization: For the ISH experiments, the cus- tom serFull Paper
2004Seo, BM;Miura, M;Gronthos, S;Bartold, PM;Batouli, S;Brahim, J;Young, M;Robey, PG;Wang, CY;Shi, S;Investigation of multipotent postnatal stem cells from human periodontal ligamentLancet (London, England)15246727BMSSCs were obtained from a commercially available resource (AllCells LLC, Berkeley, CA, USA). All primary cells used in this study were at 2-4 passages. For each experiment, the SaveFull Paper
2004Griswold, IJ;Shen, LJ;La Rosée, P;Demehri, S;Heinrich, MC;Braziel, RM;McGreevey, L;Haley, AD;Giese, N;Druker, BJ;Deininger, MW;Effects of MLN518, a dual FLT3 and KIT inhibitor, on normal and malignant hematopoiesisBlood15242881Bone marrow mononuclear cells (5 × 104) obtained either commercially (n = 3) (AllCells, San Diego, CA) or from a volunteer donor (n = 1) were plated in methylcellulose media containing 50 ng/mL recombinant human (rh) stem cell factor, 10 ng/mL granulFull Paper
2004Geddis, AE;Kaushansky, K;Megakaryocytes express functional Aurora-B kinase in endomitosisBlood15130946stimulated donors were purchased (Cambrex, East Rutherford, NJ, or AllCells, Berkley, CA). Cells were thawed according to the manufacturer's directions and cultured for 10 to 14 days in StemPro (Invitrogen) containing recombinant human TPO (rhTPO; 50Full Paper
2004Davis, BM;Humeau, L;Slepushkin, V;Binder, G;Korshalla, L;Ni, Y;Ogunjimi, EO;Chang, LF;Lu, X;Dropulic, B;ABC transporter inhibitors that are substrates enhance lentiviral vector transduction into primitive hematopoietic progenitor cellsBlood15059841(> 98% purity) derived from adult bone marrow, adult granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood, and cord blood were purchased from AllCells (Berkeley, SaveFull Paper
2004Lu, SJ;Li, F;Vida, L;Honig, GR;CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic gene expression patternBlood14962900cells were obtained. Normal human BM was purchased from AllCells (Berkeley, CA); CD34+CD38- cells were purified as described previously in this paragraph. Figure 1 shows the fluorescence-activated cell sorter (FACS) profiles that define the gates forFull Paper
2004Morrissey, P;George, T;Basiji, D;Frost, K;Ortyn, W;Williams, D;Cell Classification in Human Peripheral Blood Using the Amnis ImageStream® System.BloodHuman peripheral blood was obtained from AllCells (Berkeley, CA). Whole blood was stained for 15 minutes on ice with FITC conjugated anti-CD45 mAb and PE conjugated anti-CD14 SaveFull Paper
2004Mahadevan, D;Spier, C;Croce, K;Miller, S;George, B;Riley, C;Warner, S;Grogan, T;Miller, T;Gene Expression Profiling of Peripheral T-Cell Lymphoma (PTCL, NOS) and Comparison to Diffuse Large B-Cell Lymphoma (DLBCL).BloodFull Paper
2004Masubuchi, N;May, RD;Atsumi, R;A predictive model of human myelotoxicity using five camptothecin derivatives and the in vitro colony-forming unit granulocyte/macrophage assayClinical cancer research : an official journal of the American Association for Cancer Research15475463Cells were then washed in IMDM/5 and adjusted to the appropriate cell concentration. Human marrow samples were obtained from AllCells, LLC (Berkeley, CA) from donors that had given informed consent. The age of the 3 donors ranged from 20 to 45 years.Full Paper
2004Ibañez, V;Sharma, A;Buonamici, S;Verma, A;Kalakonda, S;Wang, J;Kadkol, S;Saunthararajah, Y;AML1-ETO decreases ETO-2 (MTG16) interactions with nuclear receptor corepressor, an effect that impairs granulocyte differentiationCancer research15231665Retrovirus-containing supernatant was added to human cord blood CD34-positive cells (AllCells) in retronectin (TaKaRa)-coated plates. The cord blood cells were cultured in Iscove's SaveFull Paper
2004Georgantas, RW;Tanadve, V;Malehorn, M;Heimfeld, S;Chen, C;Carr, L;Martinez-Murillo, F;Riggins, G;Kowalski, J;Civin, CI;Microarray and serial analysis of gene expression analyses identify known and novel transcripts overexpressed in hematopoietic stem cellsCancer research15231652The human CD34 + /CD38 − /Lin − cell subset, comprising ∼1-10% of the CD34 + cell population, contains few of the less primitive hematopoietic (lineage-committed) progenitor cells ( SaveFull Paper
2004Humeau, LM;Binder, GK;Lu, X;Slepushkin, V;Merling, R;Echeagaray, P;Pereira, M;Slepushkina, T;Barnett, S;Dropulic, LK;Carroll, R;Levine, BL;June, CH;Dropulic, B;Efficient lentiviral vector-mediated control of HIV-1 replication in CD4 lymphocytes from diverse HIV+ infected patients grouped according to CD4 count and viral loadMolecular therapy : the journal of the American Society of Gene Therapy15194057Peripheral blood from healthy donors was obtained from AllCells (Berkeley, CA, USA), and uninfected or HIV+ study subject PBMCs were isolated by Ficoll-Hypaque density gradient separation. Study subjects numbered J23 and higher and U01 had their blooFull Paper
2004Davis, BM;Humeau, L;Dropulic, B;In vivo selection for human and murine hematopoietic cells transduced with a therapeutic MGMT lentiviral vector that inhibits HIV replicationMolecular therapy : the journal of the American Society of Gene Therapy14759800progenitors were obtained from AllCells (Berkley, CA, USA) in accordance with an IRB protocol obtained by the company and were transduced and cultured in IMDM plus 1× BIT (Stem Cell Technologies, Vancouver, BC, Canada) in 25 ng/ml SCF, Flt-3L, and TPFull Paper
2004LeCouter, J;Zlot, C;Tejada, M;Peale, F;Ferrara, N;Bv8 and endocrine gland-derived vascular endothelial growth factor stimulate hematopoiesis and hematopoietic cell mobilizationProceedings of the National Academy of Sciences of the United States of America15548611Bv8 and endocrine-gland-derived VEGF (EG-VEGF), or prokineticins, are two highly related, secreted proteins that we previously described as selective angiogenic mitogens. Here we SaveFull Paper
2004Su, AI;Wiltshire, T;Batalov, S;Lapp, H;Ching, KA;Block, D;Zhang, J;Soden, R;Hayakawa, M;Kreiman, G;Cooke, MP;Walker, JR;Hogenesch, JB;A gene atlas of the mouse and human protein-encoding transcriptomesProceedings of the National Academy of Sciences of the United States of America15075390Tissue Preparation. Human tissue samples were obtained from several sources: Clinomics Biosciences (Pittsfield, MA), Clontech, AllCells (Berkeley, CA), Clonetics/BioWhittaker (Walk-ersville, MD), AMS Biotechnology (Abingdon, Oxfordshire, U.K.), and tFull Paper
2004Begovich, AB;Carlton, VE;Honigberg, LA;Schrodi, SJ;Chokkalingam, AP;Alexander, HC;Ardlie, KG;Huang, Q;Smith, AM;Spoerke, JM;Conn, MT;Chang, M;Chang, SY;Saiki, RK;Catanese, JJ;Leong, DU;Garcia, VE;McAllister, LB;Jeffery, DA;Lee, AT;Batliwalla, F;Remmers, E;Criswell, LA;Seldin, MF;Kastner, DL;Amos, CI;Sninsky, JJ;Gregersen, PK;A missense single-nucleotide polymorphism in a gene encoding a protein tyrosine phosphatase (PTPN22) is associated with rheumatoid arthritisAmerican journal of human genetics15208781(GenBank accession number NM_015967) was determined by kinetic RT-PCR analysis by use of total RNA from sorted hematopoeitic cells (AllCells) and selected tissues (Clontech), as described elsewhere (Rogge et al. 2000). Data for additional tissues areFull Paper
2004Lu, X;Yu, Q;Binder, GK;Chen, Z;Slepushkina, T;Rossi, J;Dropulic, B;Antisense-mediated inhibition of human immunodeficiency virus (HIV) replication by use of an HIV type 1-based vector results in severely attenuated mutants incapable of developing resistanceJournal of virology15194784Primary cell isolation, culture, transduction, and challenge. Peripheral blood from healthy donors was obtained from AllCells (Berkeley, Calif.), and lymphocytes were isolated by Ficoll-Hypaque density gradient separation. Cells were resuspended in bFull Paper
2004Chute, JP;Muramoto, G;Fung, J;Oxford, C;Quantitative analysis demonstrates expansion of SCID-repopulating cells and increased engraftment capacity in human cord blood following ex vivo culture with human brain endothelial cellsStem cells (Dayton, Ohio)14990859Purified human CB CD34+ cells were obtained from AllCells, LLC. (Berkeley, CA; http://www.allcells.com). HUBECs were subcultured at 1 × 105 cells/well in gelatin‐coated 6‐well plates (Costar; Cambridge, MA; http://www.corning.com) as previously descrFull Paper
2004Courtemanche, C;Huang, AC;Elson-Schwab, I;Kerry, N;Ng, BY;Ames, BN;Folate deficiency and ionizing radiation cause DNA breaks in primary human lymphocytes: a comparisonFASEB journal : official publication of the Federation of American Societies for Experimental Biology14597554Normal human T lymphocytes were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 10% dialyzed SaveFull Paper
2004Friedmann, E;Lemberg, MK;Weihofen, A;Dev, KK;Dengler, U;Rovelli, G;Martoglio, B;Consensus analysis of signal peptide peptidase and homologous human aspartic proteases reveals opposite topology of catalytic domains compared with presenilinsThe Journal of biological chemistry15385547Microarray Analysis—Human tissues, total RNA samples, and mRNA samples were obtained from Clinomics Biosciences, Inc., Clontech, AllCells, LLC, Clonetics/BioWhittaker, AMS Biotechnology, and the University of California, San Diego. The quality of allFull Paper
2004Courtemanche, C;Elson-Schwab, I;Mashiyama, ST;Kerry, N;Ames, BN;Folate deficiency inhibits the proliferation of primary human CD8+ T lymphocytes in vitroJournal of immunology (Baltimore, Md. : 1950)15322179Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen, Carlsbad, CA), supplemented with 10% dialyzed SaveFull Paper
2004Chu, PC;Wu, J;Liao, XC;Pardo, J;Zhao, H;Li, C;Mendenhall, MK;Pali, E;Shen, M;Yu, S;Taylor, VC;Aversa, G;Molineaux, S;Payan, DG;Masuda, ES;A novel role for p21-activated protein kinase 2 in T cell activationJournal of immunology (Baltimore, Md. : 1950)15187108Freshly purified PBMC (AllCells, Berkeley, CA) were incubated at 37°C for 2 h to allow the monocytes to adhere to the tissue culture flask. The cells remaining in suspension were recovered and seeded at 106 cells/ml/ well in Yssel’s medium (Gemini BiFull Paper
2004Chinnasamy, D;Fairbairn, LJ;Neuenfeldt, J;Treisman, JS;Hanson, JP;Margison, GP;Chinnasamy, N;Lentivirus-mediated expression of mutant MGMTP140K protects human CD34+ cells against the combined toxicity of O6-benzylguanine and 1,3-bis(2-chloroethyl)-nitrosourea or temozolomideHuman gene therapy15319033Lentiviral vectors are capable of efficiently transducing nondividing and slowly dividing cells, including hematopoietic stem cells, resulting in stable integration and sustained transgene SaveFull Paper
2004Lu, X;Humeau, L;Slepushkin, V;Binder, G;Yu, Q;Slepushkina, T;Chen, Z;Merling, R;Davis, B;Chang, YN;Dropulic, B;Safe two-plasmid production for the first clinical lentivirus vector that achieves >99% transduction in primary cells using a one-step protocolThe journal of gene medicine15352069Normal human blood was obtained from AllCells (Berkely, CA, USA) under the auspices of an IRB-approved protocol supplied by AllCells. Peripheral blood mononuclear cells (PBMCs) SaveFull Paper
2004Kubota, S;Kawata, K;Yanagita, T;Doi, H;Kitoh, T;Takigawa, M;Abundant retention and release of connective tissue growth factor (CTGF/CCN2) by plateletsJournal of biochemistry15598883Lower panel: Comparison of the CTGF/CCN2 contents of platelets and other CTGF/CCN2-associated tissues. Total protein extracts of normal human skin and artery were obtained commercially (Cosmo Bio Co., Ltd., Tokyo, Japan), as were human platelets fromFull Paper
2004Mashiyama, ST;Courtemanche, C;Elson-Schwab, I;Crott, J;Lee, BL;Ong, CN;Fenech, M;Ames, BN;Uracil in DNA, determined by an improved assay, is increased when deoxynucleosides are added to folate-deficient cultured human lymphocytesAnalytical biochemistry15183762Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and 1% antibiotics (penicillin/streptavidin). To ensureFull Paper
2004Murray, B;Bennett, B;Sasaki, D;Analysis of pharmacologic inhibitors of Jun N-terminal kinasesMethods in Enzymologydom donor buffy coats from the San Diego Blood Bank (San Diego, CA). Neonatal cord blood is obtained from Advanced Bioscience Resources (Alameda, CA) or as selected CD4+ cells (AllCells, Foster City, CA)Full Paper
2004Lu, SJ;Li, F;Vida, L;Honig, GR;CD34CD38 Hematopoietic Precursors Derived from Human Embryonicresearchgate.netof the total population, and a total of 2,840 CD34+ CD38- cells was obtained. Normal human BM was purchased from AllCells (AllCells, LLC, Berkeley, CA); CD34+ CD38- cells were purified as described above. Figure 1 shows the FACS profiles that defineFull Paper
2004Murmann, AE;Topology of genes in mammalian cell nuclei with special emphasis on the MLL gene and its translocation partnersThesisATCC = American Type Culture Collection, Manassas, VA. DSMZ = Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany. 3. Material 38 CD33+) were isolated by and purchased from AllCells Inc. (Berkeley, CA)Full Paper
2004Mashiyama, ST;Folate and other B-vitamins: Effects of deficiency on DNA-uracil content, chromosome damage, homocysteine, and interactions with gene polymorphismsThesisCell culture and proliferation Fresh human mononuclear cells were obtained from AllCells (Berkeley, CA) and cultured in folate-free RPMI 1640 medium (Invitrogen) supplemented with 10% dialyzed fetal bovine serum (Invitrogen) and 1% antibiotics (penicFull Paper
2003Winkler, DG;Sutherland, MK;Geoghegan, JC;Yu, C;Hayes, T;Skonier, JE;Shpektor, D;Jonas, M;Kovacevich, BR;Staehling-Hampton, K;Appleby, M;Brunkow, ME;Latham, JA;Osteocyte control of bone formation via sclerostin, a novel BMP antagonistEMBO J.14633986Mouse embryos (15.5 days old) were fixed in 4% phosphate-buffered formaldehyde pH 7.2 overnight, dehydrated and embedded in paraffin. Sections (5 µm thick) were prepared and in situ hybridization performed by Phylogeny, Inc. (Columbus, OH) using [35SFull Paper
2003Wang, T;Fan, L;Watanabe, Y;McNeill, PD;Moulton, GG;Bangur, C;Fanger, GR;Okada, M;Inoue, Y;Persing, DH;Reed, SG;L523S, an RNA-binding protein as a potential therapeutic target for lung cancerBr. J. Cancer12644826ur studies indicate that L523S may be a valuable addition to the repertoire of cancer-specific targets for the development of new immunotherapeutic and perhaps other therapeutic approaches. We thank Qualtek for their expert IHC analysis. We are gratFull Paper
2003DuHadaway, JB;Lynch, FJ;Brisbay, S;Bueso-Ramos, C;Troncoso, P;McDonnell, T;Prendergast, GC;Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: diverse sites of nuclear expression and losses in prostate cancerJ. Cell. Biochem.12532338Search for more papers by this author. Frank J. Lynch QualTek Molecular Laboratories, Newtown, Pennsylvania. Search for more papers by this author. Shawn Brisbay Search for more papers by this author. Frank J. Lynch QualTek Molecular Laboratories,Full Paper
2003Tinkelenberg, BA;Fazzone, W;Lynch, FJ;Ladner, RD;Identification of sequence determinants of human nuclear dUTPase isoform localizationExp. Cell Res.1279918015 min), DUT415 primary antibody incubation (25 min), biotinylated goat anti-mouse IgG secondary antibody (25 min), peroxidase-conjugated ABC-Elite (25 min, Vector Labs, Burlingame, CA, USA), and DAB (diaminobenzidine) chromogen (15 min, QualTek MoleFull Paper
2003Gibbs, WW;Untangling the roots of cancerSci. Am.12840947SCIENCE PHOTO LIBRARY; FRANK LYNCH QualTek Molecular Laboratories; ANDREJS LIEPINS/SPL; CNRI/SPL; SPL 1. GROWTH EVEN IN THE ABSENCE OF NORMAL "GO" SIGNALS Most normal cells wait for an external message before dividingFull Paper
2003Wang, A;Clapper, J;Guderian, JA;Foy, TM;Fanger, GR;Retter, MW;Skeiky, YA;A novel method for increasing the expression level of recombinant proteinsProtein Expr. Purif.12821330visualize antigen expression. Slides were counterstained with hematoxylin to visualize cell nuclei. We would like to acknowledge and thank Qualtek for immunohistochemistry results. FACS analysis. HEK293 cells were transientlyFull Paper
2003Weigel-Kelley, KA;Yoder, MC;Srivastava, A;Alpha5beta1 integrin as a cellular coreceptor for human parvovirus B19: requirement of functional activation of beta1 integrin for viral entryBlood12907437Human bone marrow-derived highly purified CD71 + primary erythroid progenitor cells were purchased from AllCells (Berkeley, CA). Recombinant parvovirus B19-Luc vector stocks SaveFull Paper
2003Francisco, JA;Cerveny, CG;Meyer, DL;Mixan, BJ;Klussman, K;Chace, DF;Rejniak, SX;Gordon, KA;DeBlanc, R;Toki, BE;Law, CL;Doronina, SO;Siegall, CB;Senter, PD;Wahl, AF;cAC10-vcMMAE, an anti-CD30-monomethyl auristatin E conjugate with potent and selective antitumor activityBlood12714494and goat-antihuman FITC were obtained from Jackson ImmunoResearch (West Grove, PA). Anti-CD30 mAb Ki-1 was from Accurate Chemicals (Westbury, NY). cBR96, a human chimeric IgG1κ (Seattle Genetics) was used as a chimeric, isotype-matched control. FreshFull Paper
2003Glockner, A;Optimal consistency protocols for replicated filesEighth Annual International Phoenix Conference on Computers and Communications. 1989 Conference ProceedingsPlacement continues until either allcells have been placed, or a number of iterations has beenperformed. If, during cell placement, a valid location for acell cannot be identified, the SaveFull Paper
2003Lee, J;Elkahloun, AG;Messina, SA;Ferrari, N;Xi, D;Smith, CL;Cooper, R;Albert, PS;Fine, HA;Cellular and genetic characterization of human adult bone marrow-derived neural stem-like cells: a potential antiglioma cellular vectorCancer research14695205RBCs from human adult (donors, 20-40 years old) bone marrow cells purchased from AllCells (Berkely, CA) were depleted by density gradient, and the remaining cells were plated in uncoated tissue culture plastic dishes. After 10 days of culture in 15%Full Paper
2003Yu, X;Zhan, X;D'Costa, J;Tanavde, VM;Ye, Z;Peng, T;Malehorn, MT;Yang, X;Civin, CI;Cheng, L;Lentiviral vectors with two independent internal promoters transfer high-level expression of multiple transgenes to human hematopoietic stem-progenitor cellsMolecular therapy : the journal of the American Society of Gene Therapy12788657Normal human placental/umbilical CB CD34+ cells, purchased from AllCells (San Mateo, CA), had been purified by immunomagnetic selection (Miltenyi Biotech, Auburn, CA) and were90% CD34+ upon reanalysis by FACS. CB CD34+ cells were cultured at 2.5-5 ×Full Paper
2003Thach, DC;Shaffer, KM;Ma, W;Stenger, DA;Assessing the feasibility of using neural precursor cells and peripheral blood mononuclear cells for detection of bioactive Sindbis virusBiosensors & bioelectronics12782470Fresh normal PBMC were purchased from AllCells , LLC. The cells were received in Dubecco's PBS with 0.5% bovine serum albumin (BSA) and 2 mM EDTA. The cells were spun SaveFull Paper
2003Russell, RS;Roldan, A;Detorio, M;Hu, J;Wainberg, MA;Liang, C;Effects of a single amino acid substitution within the p2 region of human immunodeficiency virus type 1 on packaging of spliced viral RNAJournal of virology14645555Allcells were supplemented with 10% fetal calf serum. Cord bloodmononuclear cells (CBMCs) were grown in RPMI 1640 supplemented with10% fetal calf serum and 20 U of interleukin- SaveFull Paper
2003Cheng, L;Hammond, H;Ye, Z;Zhan, X;Dravid, G;Human adult marrow cells support prolonged expansion of human embryonic stem cells in cultureStem cells (Dayton, Ohio)12634409BM samples collected from healthy and consenting human donors were purchased from AllCells Company (Berkeley, CA; http://www.allcells.com). The use of anonymous primary human cells for laboratory research was approved by the Internal Review Board ofFull Paper
2003Hsu, DH;Paz, P;Villaflor, G;Rivas, A;Mehta-Damani, A;Angevin, E;Zitvogel, L;Le Pecq, JB;Exosomes as a tumor vaccine: enhancing potency through direct loading of antigenic peptidesJournal of immunotherapy (Hagerstown, Md. : 1997)12973033CD14+ monocyte-enriched adherent cells from healthy donor leukopacks acquired from Allcells (Berkeley, CA) were cultured in ultrafiltered AIMV medium (Invitrogen) supplemented SaveFull Paper
2003Hofstra, CL;Desai, PJ;Thurmond, RL;Fung-Leung, WP;Histamine H4 receptor mediates chemotaxis and calcium mobilization of mast cellsThe Journal of pharmacology and experimental therapeutics12626656Human HMC-1 and WEHI-3 cells were purchased from American Type Culture Collection (Manassas, VA). Human CD34 cord blood cells were from AllCells, LLC (Berkeley, CA). Basophil enrichment kit and serum-free medium were from Stem Cells Technologies (VanFull Paper
2003Greco, NJ;Lee, WR;Moroff, G;Increased transmigration of G-CSF-mobilized peripheral blood CD34+ cells after overnight storage at 37 degrees CTransfusion14617318Samples from UCSD and AllCells LLC were shipped overnight (ON) at 1 to 6C and utilized in experiments upon receipt the following morning (referred to as Day 1). PBPC samples from SaveFull Paper
2003Scherer, A;Krause, A;Walker, JR;Sutton, SE;Serón, D;Raulf, F;Cooke, MP;Optimized protocol for linear RNA amplification and application to gene expression profiling of human renal biopsiesBioTechniques12661160Human PBMCs were purchased from Allcells (Oakland, CA, USA). Cells were subjected to RNA purification with RNeasy® (Qiagen, Valencia, CA, USA). The RNA was eluted with 80 µL RNase-free water (Ambion,Full Paper
2003Onguru, O;Celasun, B;Gunhan, O;Comparison of DNA ploidy and nuclear morphometric parameters with the conventional prognostic factors in transitional cell carcinomasThe Tohoku journal of experimental medicine12703658ThemeasurementofNA10in thisstudydemonstratedthemeannucleararea ofthebiggest10cellsamong the allcells measuredatthenucleussuspensionforeach case. Thus, SaveFull Paper
2003Yamaguchi, H;Aridgides, LJ;Zeng, W;Osgood, C;Young, NS;Liu, JM;Genetic and transcriptional analysis of spindle checkpoint genes in bone marrow failure patientsBlood cells, molecules & diseases12737950peripheral blood (PB)-MNCs was extracted using an RNAzol kit (Biotex Laboratories, Houston, TX) or Trizol (Life Technologies). Total RNA from purified T cell, B cell, and neutrophil fractions was purchased (AllCells, LLC, Berkeley, CA)Full Paper
2003Christensen, JL;Mechanisms and mediators of hematopoietic stem cell fateThesisHuman bone marrow was obtained from normal donors (Allcells, Oakland, CA). Bone marrow was diluted in Hanks balanced salt solution, and 20ml was layered onto 15ml of ficoll (Ficoll-Paque PLUS (Amersham Phamacia Biotech), and centrifuged for 30 minuteFull Paper
2003Chu, P;Pardo, J;Zhao, H;Li, CC;Pali, E;Shen, MM;Qu, K;Yu, SX;Huang, BC;Yu, P;Masuda, ES;Molineaux, SM;Kolbinger, F;Aversa, G;de Vries, J;Payan, DG;Liao, XC;Systematic identification of regulatory proteins critical for T-cell activationJournal of biology129749811,599 of its open reading frame; and TRAC-1, base pairs 1-509 of its open reading frame. Culture and infection of primary T lymphocytes Commercially available primary blood mononuclear cells (PBMC; AllCells LLC, Berkeley, USA) were cultured in RPMIFull Paper
2003Knowles, S;Woods, R;McWhirter, J;McCanny, J;Bit-level systolic arrays for IIR filtering[1988] Proceedings. International Conference on Systolic Arraysthroughout this paper.,The application contains four main functions as follows:,1),startPlay,function calls,setup,to set the,dimension of all DOM elements with a class name of,allCells,;,2), SaveFull Paper
2003Chow, DC;Effects of surface-immobilized stem cell factor on the differentiation and proliferation of hematopoietic cellsThesisgnetic cell separation system (Miltenyl Biotec GmbH, Bergisch Gladbach, Germany) (AllCells, Berkely, CA). Cells were cultured in the presence of soluble SCF (100 ng/ml) or surface-immobilized SCF in serum-free medium (XVIVO20. Cambrex Bio Science, WaFull Paper
2002Elias, PM;Ahn, SK;Denda, M;Brown, BE;Crumrine, D;Kimutai, LK;Kömüves, L;Lee, SH;Feingold, KR;Modulations in epidermal calcium regulate the expression of differentiation-specific markersJ. Invest. Dermatol.12445203from Vector Laboratories (Burlingame, CA). Peroxidase activity was revealed with diaminobenzidine (QualTek Laboratories, Santa Barbara, CA), followed by counterstaining with methyl green. Omission of the first antibodiesFull Paper
2002Xie, Z;Komuves, L;Yu, QC;Elalieh, H;Ng, DC;Leary, C;Chang, S;Crumrine, D;Yoshizawa, T;Kato, S;Bikle, DD;Lack of the vitamin D receptor is associated with reduced epidermal differentiation and hair follicle growthJ. Invest. Dermatol.11851870Peroxidase activity was revealed with diaminobenzidine substrate (QualTek Laboratories, Santa Barbara, CA) followed by counterstaining with methyl green or hematoxylin. Omitting the first antibodies resulted in no signal, indicating the specificity oFull Paper
2002Fanger, GR;Houghton, RL;Retter, MW;Hendrickson, RC;Babcook, J;Dillon, DC;Durham, MD;Reynolds, LD;Johnson, JC;Carter, D;Fleming, TP;Roche, PC;Persing, DH;Reed, SG;Detection of mammaglobin in the sera of patients with breast cancerTumour Biol.12499777Acknowledgments We thank Frank Lynch at Qualtek for immunohistochemistry efforts and expertise. Work was supported in part by USPHS grant CA76227 (TPF), and NIH grants CA-75794 (RLH) and CA- 86673 (DCD) References 1 Donegan W, Spratt J: Cancer of theFull Paper
2002Kumar, S;Hanning, CR;Brigham-Burke, MR;Rieman, DJ;Lehr, R;Khandekar, S;Kirkpatrick, RB;Scott, GF;Lee, JC;Lynch, FJ;Gao, W;Gambotto, A;Lotze, MT;Interleukin-1F7B (IL-1H4/IL-1F7) is processed by caspase-1 and mature IL-1F7B binds to the IL-18 receptor but does not induce IFN-gamma productionCytokine12096920Full Paper
2002Mulero, JJ;Boyle, BJ;Bradley, S;Bright, JM;Nelken, ST;Ho, TT;Mize, NK;Childs, JD;Ballinger, DG;Ford, JE;Rupp, F;Three new human members of the lipid transfer/lipopolysaccharide binding protein family (LT/LBP)Immunogenetics12185532The BPIL2 probe was derived from a 414-nt coding sequence between the primers 5'-CATTATTGCAAGTGAAGTCAAAGC-3' and 5'-ACCATGA- AGGGCTGGGACAAGATG-3'. QualTek Molecular labs (Santa Barbara, Calif.) performed the automated in situ hybridization us- ing aFull Paper
2002Zhao, Z;Yaar, R;Ladd, D;Cataldo, LM;Ravid, K;Overexpression of A3 adenosine receptors in smooth, cardiac, and skeletal muscle is lethal to embryosMicrovasc. Res.11749073Page 4. tion, generating either a sense or an antisense strand template for in vitro transcription (with EcoRV or HindIII, respectively). In situ hybridization was performed by Dr. Adel Mikhail (Phylogeny, Inc., Columbus, OH) as described by Lyons etFull Paper
2002Hevehan, DL;Miller, WM;Papoutsakis, ET;Differential expression and phosphorylation of distinct STAT3 proteins during granulocytic differentiationBlood11861277Fresh G-CSF-mobilized peripheral blood CD34 + cells from normal donors were obtained from AllCells (Foster City, CA) within 24 hours after collection. The experimental design and SaveFull Paper
2002Cui, Y;Golob, J;Kelleher, E;Ye, Z;Pardoll, D;Cheng, L;Targeting transgene expression to antigen-presenting cells derived from lentivirus-transduced engrafting human hematopoietic stem/progenitor cellsBlood11781219Human G-CSF-mobilized peripheral blood or cord blood CD34+ cells were obtained from AllCells (San Mateo, CA). CD34+ cells were purified by immunomagnetic selection (Miltenyi Biotec, Auburn, CA) and then cryopreserved. The purity of CD34+ cells was usFull Paper
2002Tuttle, G;Fallahi, S;Abidi, A;An 8 b CMOS vector A/D converter1993 IEEE International Solid-State Circuits Conference Digest of Technical PapersThe two pMOSFETsin allcells are diode connected, and the nMOSFET is biased by a globally-applied reference gate voltage. The input is then switched to the prompt vector X, and the SaveFull Paper
2002Kang, SJ;Cresswell, P;Regulation of intracellular trafficking of human CD1d by association with MHC class II moleculesThe EMBO journal11927549DCs were derived from monocytes as described (Sallusto and Lanzavecchia, 1994). Briefly, monocytes from peripheral blood mononuclear cells (8 × 108 cells) (AllCells, LLC, Berkeley, CA) were attached to tissue culture flasks in 20% FBS/RPMI 1640 twiceFull Paper
2002Manz, MG;Miyamoto, T;Akashi, K;Weissman, IL;Prospective isolation of human clonogenic common myeloid progenitorsProceedings of the National Academy of Sciences of the United States of America12193648Bone Marrow and Cord Blood Samples. Fresh human bone marrow (donor age, 20-47 years) was obtained with informed consent from healthy volunteers (AllCells, Berkeley, CA), allogeneic bone marrow donors (Department of Bone Marrow Transplantation, StanfoFull Paper
2002Mahmud, DL;G-Amlak, M;Deb, DK;Platanias, LC;Uddin, S;Wickrema, A;Phosphorylation of forkhead transcription factors by erythropoietin and stem cell factor prevents acetylation and their interaction with coactivator p300 in erythroid progenitor cellsOncogene11896584Primary human erythroid progenitors were derived by in vivo culture of CD34+ cells isolated from peripheral blood. Growth factor mobilized peripheral blood collected from normal donors was purchased from AllCells, LLC (Berkeley, CA, USA). These bloodFull Paper
2002Yang, H;Miller, WM;Papoutsakis, ET;Higher pH promotes megakaryocytic maturation and apoptosisStem cells (Dayton, Ohio)12110701Cultures were initiated with adult human CD34+ cells selected from apheresis products obtained from normal donors (AllCells; Berkeley, CA; http://www.allcells.com) or cancer patients (Northwestern Memorial Hospital, Chicago, IL) following stem cell mFull Paper
2002Kim, H;Whartenby, KA;Georgantas, RW;Wingard, J;Civin, CI;Human CD34+ hematopoietic stem/progenitor cells express high levels of FLIP and are resistant to Fas-mediated apoptosisStem cells (Dayton, Ohio)11897874Human placental/umbilical cord blood (CB) CD34+ cell preparations, purified by immunomagnetic selection following manufacturer's instructions (Miltenyi Biotechnologies; Auburn, CA; http://www.miltenyibiotec.com) and then cryopreserved, were purchasedFull Paper
2002Welm, AL;Timchenko, NA;Ono, Y;Sorimachi, H;Radomska, HS;Tenen, DG;Lekstrom-Himes, J;Darlington, GJ;C/EBPalpha is required for proteolytic cleavage of cyclin A by calpain 3 in myeloid precursor cellsThe Journal of biological chemistry12105198Bone marrow was isolated from both femurs of adult animals as described (25). CD33+ and CD15+ bone marrow cells were obtained from AllCells, L.L.C. skeletal muscle was harvested from adult animals as a control for calpain 3 Northern analysisFull Paper
2002Jacobs-Helber, SM;Abutin, RM;Tian, C;Bondurant, M;Wickrema, A;Sawyer, ST;Role of JunB in erythroid differentiationThe Journal of biological chemistry11726656Growth factor-mobilized peripheral blood collected from normal donors was purchased from AllCells, LLC (Berkeley, CA). CD34 + cells were isolated from growth factor-mobilized SaveFull Paper
2002Harrisson, F;Cellular origin of the basement membrane in embryonic chicken/quail chimeras.International Journal of Developmental Biologysuch as Quailnucleoli, are not clear in allcells. The drawings show the place where the photomicrographs were taken. The dashed area indicates the locatIOn of the mesoblast. SaveFull Paper
2002Sharma, A;The role of the hiwi gene in human hematopoiesisThesisLouis, MO) and stained first with biotin-conjugated K6.1 (20 ug/ml). washed, and labeled with Miltenyi streptavidin-conjugated iron microbeads (Miltenyi Biotec) and selected as described above according to manufacturer's instructions. Cord blood (CB)Full Paper
2001Afar, DE;Vivanco, I;Hubert, RS;Kuo, J;Chen, E;Saffran, DC;Raitano, AB;Jakobovits, A;Catalytic cleavage of the androgen-regulated TMPRSS2 protease results in its secretion by prostate and prostate cancer epitheliaCancer Res.11245484We thank Drs. Robert Eisenman, Owen Witte, and William Isaacs for helpful suggestions and critical reading of the manuscript; Frank Lynch and Page Erickson at QualTek Molecular Laboratories for their expertise in immunohistochemistry; and Celeste JonFull Paper
2001Xu, J;Kalos, M;Stolk, JA;Zasloff, EJ;Zhang, X;Houghton, RL;Filho, AM;Nolasco, M;Badaró, R;Reed, SG;Identification and characterization of prostein, a novel prostate-specific proteinCancer Res.11245466Immunohistochemical Studies. Immunohistochemical staining was performed on formalin-fixed, paraffin-embedded tissues by QualTek Molecular Laboratories using the prostein-specific mouse monoclonal antibody 10E3- G4-D3. Chromosome Localization. The GenFull Paper
2001Wang, T;Fan, L;Watanabe, Y;McNeill, P;Fanger, GR;Persing, DH;Reed, SG;L552S, an alternatively spliced isoform of XAGE-1, is over-expressed in lung adenocarcinomaOncogene11753648An affinity purified anti-L552S polyclonal antibody was subjected to immunohistochemistry analysis by QualTek Molecular Laboratories (Santa Barbara, CA, USA). In all cases, four micron sections of formalin fixed, paraffin-embedded tissues were used.Full Paper
2001Lin, H;Ho, AS;Haley-Vicente, D;Zhang, J;Bernal-Fussell, J;Pace, AM;Hansen, D;Schweighofer, K;Mize, NK;Ford, JE;Cloning and characterization of IL-1HY2, a novel interleukin-1 family memberJ. Biol. Chem.11278614The anti-CD20 monoclonal antibody was purchased from DAKO (Carpenteria, CA), and the anti-Ki67 was obtained from Coulter Immunotech (Miami, FL). Immunohistochemistry was performed at QualTek Molecular Systems, IncFull Paper
2001Kutty, RK;Kutty, G;Samuel, W;Duncan, T;Bridges, CC;El-Sherbeeny, A;Nagineni, CN;Smith, SB;Wiggert, B;Molecular characterization and developmental expression of NORPEG, a novel gene induced by retinoic acidJ. Biol. Chem.11042181Immunoreactivity was detected by peroxidase staining using reagents from Dako Corp. (Carpinteria, CA). In Situ Hybridization. In situ hybridization was performed by Phylogeny Inc. following the procedure of Lyonset al. (18) as described before (19)Full Paper
2001Suerbaum, S;Lohrengel, M;Sonnevend, A;Ruberg, F;Kist, M;Allelic diversity and recombination in Campylobacter jejuniJ. Bacteriol.11274115Different genes show network-like and tree-like phylogeny in C. jejuni. In order to further analyze the population structure of C. jejuni, we used split decomposition analysis. Split decomposition was originally described by Bandelt and Dress in 1992Full Paper
2001Taylor, RE;Shows, KH;Zhao, Y;Pittler, SJ;A PDE6A promoter fragment directs transcription predominantly in the photoreceptorBiochem. Biophys. Res. Commun.11401494Mannheim). An outside commercial company (Qualtek Inc., CA) confirmed results obtained in-house using the same exper- imental probes as above with proprietary in situ hybridization re- agents and the following conditionsFull Paper
2001Ladner, RD;The role of dUTPase and uracil-DNA repair in cancer chemotherapyCurr. Protein Pept. Sci.12374095await further investigation. ACKNOWLEDGEMENTS Special thanks is extended to Dr. Frank J. Lynch at QualTek Molecular Laboratories, Santa Barbara, CA, for expertise in immunohistochemistry. Special thanks are extendedFull Paper
2001Gao, Z;Golob, J;Tanavde, VM;Civin, CI;Hawley, RG;Cheng, L;High levels of transgene expression following transduction of long-term NOD/SCID-repopulating human cells with a modified lentiviral vectorStem cells (Dayton, Ohio)11359950Normal human cord blood (CB) CD34+ cells were purchased from either AllCells (San Mateo, CA; http://www.purecell.com) or Poietics/BioWhittaker (Gaithersburg, MD; http://www.biowhittaker.com/). CD34+ cells were purified by immunomagnetic selection (Mi
2001Bonfoco, E;Li, E;Kolbinger, F;Cooper, NR;Characterization of a novel proapoptotic caspase-2- and caspase-9-binding proteinThe Journal of biological chemistry11350957Positively selected, CD19+ MACS-purified human B cells (AllCells, San Jose, CA) were resuspended at 1 × 106 cells/ml in Yssel's medium without antibody (Gemini Bioproducts, Calabasas, CA) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT).
2001Alonso, A;Saxena, M;Williams, S;Mustelin, T;Inhibitory role for dual specificity phosphatase VHR in T cell antigen receptor and CD28-induced Erk and Jnk activationThe Journal of biological chemistry11085983Human bone marrow was purchased from AllCells LLC (Foster City, CA). Bone marrow leukocytes were obtained by hypotonic lysis of the erythrocytes in water for 1 min followed by washing of the cells in RPMI medium. Peripheral blood lymphocytes (∼80% T
2001西田耕介, ;高城敏美, ;木下進一, ;辻正, ;燃料電池・ガスタービン複合発電システムの性能解析 (熱工学, 内燃機関, 動力など)日本機械学会論文集 B 編段目の電 のみに蒸気を投入する方式(1段目蒸気投入 方式,1stcel1steamadditionform)は,全 段の電 に蒸 気を投入する方式(全段蒸気投入方式,Allcells Steam additionform)よりも単位燃料 Save
2001Mostafa, SS;Papoutsakis, ET;Miller, WM;Oxygen tension modulates the expression of cytokine receptors, transcription factors, and lineage-specific markers in cultured human megakaryocytesExperimental hematology11438210Cultures were initiated with adult human CD34+ cells selected from apheresis products obtained from normal donors mobilized with G-CSF (Poietics Technologies, Gaithersburg, MD, USA, and AllCells, Foster City, CA, USA). CD34+ cells were selected 1 day
2000Xu, J;Stolk, JA;Zhang, X;Silva, SJ;Houghton, RL;Matsumura, M;Vedvick, TS;Leslie, KB;Badaro, R;Reed, SG;Identification of differentially expressed genes in human prostate cancer using subtraction and microarrayCancer Res.10749139Truncated P503S (amino acid 113–241) was cloned into pET32b (Novagen) and expressed in E. coli as thioredoxin fusion proteins with a histidine tag. P503S was purified by nickel chromatography, digested with thrombin, and further purified by reverse-p
2000Kömüves, LG;Hanley, K;Lefebvre, AM;Man, MQ;Ng, DC;Bikle, DD;Williams, ML;Elias, PM;Auwerx, J;Feingold, KR;Stimulation of PPARalpha promotes epidermal keratinocyte differentiation in vivoJ. Invest. Dermatol.10951268purchased from Vector (Burlingame, CA). Peroxidase activity was revealed with diaminobenzidine (QualTek Laboratories, Santa Barbara, CA), followed by counterstaining with methyl green. Omitting the first antibodies resulted
2000Johnson, LV;Ozaki, S;Staples, MK;Erickson, PA;Anderson, DH;A potential role for immune complex pathogenesis in drusen formationExp. Eye Res.10865992ANDERSONa a Center for the Study of Macular Degeneration, Neuroscience Research Institute, University of California, Santa Barbara, CA 93106, USA, b QualTek Molecular Laboratories, Santa Barbara, CA 93111, USA (Received
2000Yeung, G;Mulero, JJ;McGowan, DW;Bajwa, SS;Ford, JE;CD39L2, a gene encoding a human nucleoside diphosphatase, predominantly expressed in the heartBiochemistry11041856the manufacturer. Automated in situ hybridization was performed by QualTek Molecular labs (Santa Barbara, CA) using a modified version of a previously published procedure (17). The Ventana Medical Systems, Inc. (Tucson
2000Kralchevsky, PA;Nagayama, K;Capillary interactions between particles bound to interfaces, liquid films and biomembranesAdvances in colloid and interface science10768480This dissertation studies cellular rearrangements in tissues and attempts to establish the role of physical properties of cells, tissues and membranes in several biological phenomena. Save
2000Pettus, P;Modeling Fecal Bacteria in Oregon Coastal Streams Using Spatially Explicit Watershed CharacteristicsPortland State UniversityPathogens, such as Escherichia coli and fecal coliforms, are causing the majority of water quality impairments in US, making up∼ 87% of this grouping's violations. Predicting and Save
2000Mostafa, SS;Effects of culture oxygen tension on human megakaryocytes: A phenotypic and mechanistic studyThesisAll of the molecular studies were conducted using CD34+ cells from fresh apheresis products (~ 2% CD34+ cells) collected from G-CSF mobilized normal donors (Poietics Technologies, Gaithersburg, MD and AllCells, Foster City, CA). PB instead of BM cell
1999Hubert, RS;Vivanco, I;Chen, E;Rastegar, S;Leong, K;Mitchell, SC;Madraswala, R;Zhou, Y;Kuo, J;Raitano, AB;Jakobovits, A;Saffran, DC;Afar, DE;STEAP: a prostate-specific cell-surface antigen highly expressed in human prostate tumorsProc. Natl. Acad. Sci. U.S.A.10588738Human tissues for RNA and protein analyses were obtained from the Human Tissue Resource Center at the University of California, Los Angeles; from the National Disease Research Interchange (Philadelphia); and from QualTek Molecular Labs (Santa Barbara
1999Kömüves, LG;Hanley, K;Jiang, Y;Katagiri, C;Elias, PM;Williams, ML;Feingold, KR;Induction of selected lipid metabolic enzymes and differentiation-linked structural proteins by air exposure in fetal rat skin explantsJ. Invest. Dermatol.10084306purchased from Vector (Burlingame, CA). Peroxidase activity was revealed with DAB substrate (QualTek Laboratories, Santa Barbara, CA) followed by counterstaining with methyl green. Omitting the first antibodies resulted
1999Hu, MC;Wang, YP;Mikhail, A;Qiu, WR;Tan, TH;Murine p38-delta mitogen-activated protein kinase, a developmentally regulated protein kinase that is activated by stress and proinflammatory cytokinesJ. Biol. Chem.10066767From the ‡ Department of Cell Biology, Amgen, Inc., Thousand Oaks, California 91320, ¶ Phylogeny, Inc., Columbus, Ohio 43212, and the ‖ Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030. Next Section. Abstrac
1999Widder, E;Johnsen, S;Bernstein, S;Case, J;Neilson, D;Thin layers of bioluminescent copepods found at density discontinuities in the water columnMarine BiologyCommunicated by NH Marcus, Tallahassee EA Widder (8) a S. Johnsen Harbor Branch Oceanographic Institution, Fort Pierce, Florida 34946, USA Fax: 001 (0)561 468-0757 e-mail: widder@hboi.edu SA Bernstein QualTek, Santa Barbara, California 93111, USA JF
1999Payre, F;Vincent, A;Carreno, S;ovo/svb integrates Wingless and DER pathways to control epidermis differentiationNature10421370Similar results were obtained in allcells of the embryonic ventral epidermis tested, using a variety of Gal4 drivers. The denticle production induced by ectopic svb expression does not Save
1998Weymann, R;Jannuzi, B;Lu, L;Bahcall, J;Bergeron, J;Boksenberg, A;Hartig, G;Kirhakos, S;Sargent, W;Savage, B;Schneider, D;Turnshek, D;Wolfe, A;TheHubble Space TelescopeQuasar Absorption Line Key Project. XIV. The Evolution of Lyα Absorption Lines in the Redshift Intervalz= 0-1.5The Astrophysical JournalWe present the results of an analysis of the rate of evolution of the Lyα absorption lines in the redshift interval 0.0 to~ 1.5 based upon a sample of 987 Lyα absorption lines identified in Save
1997Schmidt-Erfurth, U;Diddens, H;Birngruber, R;Hasan, T;Photodynamic targeting of human retinoblastoma cells using covalent low-density lipoprotein conjugatesBritish journal of cancer9000598Firstly, the apoB/E receptor, which is present on allcells, is the main mechanism by which cells gain exogenous cholesterol and can be regulated up or down to attain cholesterol Save
1997Surinya, KH;Heme biosynthesis in erythroid cells : transcriptional egulation of the human 5=aminolevulinate synthase 2 gene / a thesis submitted to the University of Adelaide for the degree of Doctor of Philosophy by Katharina Helen SurinyaThesisThere are two isozymes for ALAS: a housekeeping form expressed in allcells (ALAS1), and an erythroid-specific form (ALAS2) responsible for the higher protoporphyrin synthesis in Save
1996Kavanagh, K;Whittaker, PA;Application of protoplast fusion to the nonconventional yeastEnzyme and microbial technologyMicrobio/. 1983. 129, 337 IL3377 Necas. 0.. Svoboda, A. and Havelkova, M. Mechanism of regen- eration of yeast protoplasts: Formation of the cell wall in %hixmc- ckcwon~~~~es pornbr. Folio. Bio[. 1968, 14, 80-85 Necan. 0. Cell wall synthesis in yeast
1996Evers, BM;Zhou, Z;Dohlen, V;Rajaraman, S;Thompson, JC;Townsend, CM;Fetal and neoplastic expression of the neurotensin gene in the human colonAnnals of surgery8651737This gene is present in allcells in the body, but, interestingly, it is differentially expressed. As shown in this study, it tends to be expressed in the fetal colon and in the adult small bowel. Save
1996Salinas, E;Abbott, LF;A model of multiplicative neural responses in parietal cortexProceedings of the National Academy of Sciences of the United States of America8876244Thesetwo parameterswill either be the same for allcells, in which case we refer to them as m andb, orwill be chosen from Gaussian distributions with fixed means and standard Save
1996Nwoga, JC;Sniffen, JC;Peña-Rasgado, C;Kimler, VA;Rasgado-Flores, H;Effect of pentachlorophenol on calcium accumulation in barnacle muscle cellsThe Journal of physiology9011605Allcells were mounted in the experimental chamber in the presence of NaSWOCa (see Methods). Except for the case where the cells remained in NaSWOCa, cells were pre-incubated Save
1996Kineman, RD;Gettys, TW;Frawley, LS;Role of guanine nucleotide-binding proteins, Gi alpha 3 and Gs alpha, in dopamine and thyrotropin-releasing hormone signal transduction: evidence for competition and commonalityThe Journal of endocrinology8778223Given that SLO is an invasive agent and could result in cell lysis (approximately 1-2% of allcells lyse following SLO treatment), the RHPA allows for the evaluation ofPRL release from Save
1996Berry, RM;Berg, HC;Torque generated by the bacterial flagellar motor close to stallBiophysical journal8968619
1996Goodman, MB;Art, JJ;Positive feedback by a potassium-selective inward rectifier enhances tuning in vertebrate hair cellsBiophysical journal8804626
1996Laughlin, MJ;Christiansen, NP;Herzig, GP;Blumenson, L;Bonney, D;Stewart, CC;CD34 progenitor cell subset analyses in normal human bone marrow and marrow harvested after intermediate-dose chemotherapyCytometry8979021CD34 + cells exhibiting high side scatter, R8 (to include allcells to theright ofR1)were >95% basophils(Fig. 2f). Most mature basophils exhibit low side scatter because their granules Save
1995Wang, XJ;Golomb, D;Rinzel, J;Emergent spindle oscillations and intermittent burst firing in a thalamic model: specific neuronal mechanismsProceedings of the National Academy of Sciences of the United States of America7777551The histograms were obtained by computing the mean bursting rates of allcells averaged over a timeinterval of 10 s. (D) Normalized interburst interval (IBI) histogram (bin = 10 ms) Save
1995Nooter, K;Boersma, AW;Oostrum, RG;Burger, H;Jochemsen, AG;Stoter, G;Constitutive expression of the c-H-ras oncogene inhibits doxorubicin-induced apoptosis and promotes cell survival in a rhabdomyosarcoma cell lineBritish journal of cancer7880739At 1 JM doxorubicin - a concentration that gives more than 4 log cell kill in a clonogenic assay on the R2NEO cells (Figure 3) - 96 h after drug incubation practically allcells were Save
1995Hofstadler, SA;Swanek, FD;Gale, DC;Ewing, AG;Smith, RD;Capillary electrophoresis-electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry for direct analysis of cellular proteinsAnalytical chemistry7741217The initial cell population has a significant spatial distribution, and it is unlikely that allcells lysed simultaneously, leading to band dispersion greater than that due to the initial spatial Save
1995Juckett, MB;Balla, J;Balla, G;Jessurun, J;Jacob, HS;Vercellotti, GM;Ferritin protects endothelial cells from oxidized low density lipoprotein in vitroThe American journal of pathology7677189In allcells, ferritin synthesis is tightly coupled with the intracellular iron concentration, which presumably prevents expansion of a potentially damaging free iron pool. We have found that Save
1995Preudhomme, C;Lepelley, P;Lovi, V;Zandecki, M;Cosson, A;Fenaux, P;T-cell acute lymphoblastic leukemia occurring in the course of B cell chronic lymphocytic leukemia: a case reportLeukemia & lymphoma8535207In the six cases studied, similar immunoglobulin chain isotypes were found in the CLL and ALLcells, while identical cytogenetic abnormalities were present in CLL and ALL in the Save
1994Uckun, F;Downing, J;Chelstrom, L;Gunther, R;Ryan, M;Simon, J;Carroll, A;Tuel-Ahlgren, L;Crist, W;Human t(4;11)(q21;q23) acute lymphoblastic leukemia in mice with severe combined immunodeficiencyBlood
1994Shikama, N;Ackermann, R;Brack, C;Protein synthesis elongation factor EF-1 alpha expression and longevity in Drosophila melanogasterProceedings of the National Academy of Sciences of the United States of America8183891Therefore, regulation of protein synthesis can beconsidered as one key homeostatic function common to allcells. A decrease in the rate ofprotein synthesis with advancing age has been Save
1994Yao, A;Rubin, H;A critical test of the role of population density in producing transformationProceedings of the National Academy of Sciences of the United States of America8052648In a competentpopulation, allcells have the capacityto produce some transformed foci if kept under the constraint of confluence and/or reduced serum concentration for extended Save
1994Ingenbleek, Y;Young, V;Transthyretin (prealbumin) in health and disease: nutritional implicationsAnnual review of nutrition7946531the fteld of both thyroid hormones (175, 176), whose three speciftc carrier proteins act as buffering reservoirs, thereby allowing the uniform distribution of the horн mones among allcells Save
1994Ferreira, GC;Mammalian ferrochelatase. Overexpression in Escherichia coli as a soluble protein, purification and characterizationThe Journal of biological chemistry8308010dance of ferrochelatase in mitochondria and its difficulty of Although ferrochelatase is present in allcells, it is predomi- solubilization from the membrane environment (1,2). In this nantly Save
1994McDonnell, DP;Goldman, ME;RU486 exerts antiestrogenic activities through a novel progesterone receptor A form-mediated mechanismThe Journal of biological chemistry8163495In the pri- suggests that estrogen receptor-mediated activation of tranmate uterus, for instance, in addition to functioning an an- scription is not identical in allcells. Since PR-A is not Save
1994Skiba, PJ;Keesler, GA;Tabas, I;Interferon-gamma down-regulates the lipoprotein(a)/apoprotein(a) receptor activity on macrophage foam cells. Evidence for disruption of ligand-induced receptor recycling by interferon-gammaThe Journal of biological chemistry8083207Allcells were then assayed for 5 h 3 (in the absence IFN-y) for lZ5I-r-apo(a) degradation. C,resident mouse peritoneal macrophages werepreincubated for 3 days in DMEM, 10%LPDS, Save
1994Cazenave, C;Frank, P;Toulme, JJ;Büsen, W;Characterization and subcellular localization of ribonuclease H activities from Xenopus laevis oocytesThe Journal of biological chemistry7929207to be primarily concentrated in the germinal drawn from the various attempts to characterize these envesicle, but around of this activity was detected 5% in the zymes further: allcells Save
1994Carrel, F;Dharmawardhane, S;Clark, AM;Powell-Coffman, JA;Firtel, RA;Spatial and temporal expression of the Dictyostelium discoideum G alpha protein subunit G alpha 2: expression of a dominant negative protein inhibits proper prestalk to stalk differentiationMolecular biology of the cell8186466Using the full-length promoter, aggregates showed uniform staining during aggregation and mound formation, suggesting that Ga2 is expressed in allcells at this stage of development, Save
1994Aoyagi, M;Furusawa, S;Waga, K;Tsunogake, S;Shishido, H;Suppression of normal hematopoiesis in acute leukemia: effect of leukemic cells on bone marrow stromal cells and hematopoietic progenitor cellsInternal medicine (Tokyo, Japan)7949632Effects ofleukemic cells (LC) onbone marrowstromal cells and myeloid progenitor cells (CFUC) were studied in vitro, using LClines with different lineage characteristics. LCand/or LC- Save
1994Van Hamme, LJ;Associative and statistical accounts of cue competition in causality judgmentsThesisThis expression equ ally utilizes the inform ation in allcells of the ta b le and re p re sen ts one measure of the tru e contingency between the even ts. Although developed w ith in qu ite Save
1993Kohl, NE;Mosser, SD;deSolms, SJ;Giuliani, EA;Pompliano, DL;Graham, SL;Smith, RL;Scolnick, EM;Oliff, A;Gibbs, JB;Selective inhibition of ras-dependent transformation by a farnesyltransferase inhibitorScience (New York, N.Y.)8316833Apparently, lovastatin's growth-inhibitory effects involve multiple mevalonate-dependent pathways that are required for allcells to growand divide. The specificity observed with L- Save
1993Campana, D;Coustan-Smith, E;Manabe, A;Buschle, M;Raimondi, SC;Behm, FG;Ashmun, R;Aricò, M;Biondi, A;Pui, CH;Prolonged survival of B-lineage acute lymphoblastic leukemia cells is accompanied by overexpression of bcl-2 proteinBlood8427984Overexpression of bcl-2 delays the onset of apoptosis in lymphohematopoietic cells. We measured levels of bcl-2 protein in normal and leukemic human B-cell progenitors with a Save
1993Ruiz-Gómez, M;Ghysen, A;The expression and role of a proneural gene, achaete, in the development of the larval nervous system of DrosophilaThe EMBO journal8458326The late expression is abnormal in that most or allcells of the clusters shift to, and stay at, the high level of expression that is normally observed only in the progenitor cell. We conclude Save
1993Rice, NR;Ernst, MK;In vivo control of NF-kappa B activation by I kappa B alphaThe EMBO journal8223478Whatever the basis for IxBct's instability in the cell lines examined here, there is no apriori reason to think that allcells have such unstable IxBa.For example, if the hypothetical kinase Save
1993Camplejohn, RS;Brock, A;Barnes, DM;Gillett, C;Raikundalia, B;Kreipe, H;Parwaresch, MR;Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cellsBritish journal of cancer7682430There is considerable interest in immunohistochemical markers of proliferation which are suitable for use on routinely fixed clinical material. The novel proliferation-associated antibody Save
1993Art, JJ;Fettiplace, R;Wu, YC;The effects of low calcium on the voltage-dependent conductances involved in tuning of turtle hair cellsThe Journal of physiology8308720Allcells tuned to very low frequencies also possessed an inward rectifier (see records in Fig. C) revealed with hyperpolarizing pulses negativeto the K+ equilibrium potential. An inward Save
1993Cociancich, S;Ghazi, A;Hetru, C;Hoffmann, JA;Letellier, L;Insect defensin, an inducible antibacterial peptide, forms voltage-dependent channels in Micrococcus luteusThe Journal of biological chemistry7690029There are at least two possible explanations for this observation: either allcells are not hit by defensin, or they are all hit, but areonly partially depleted of K'. A partial depletion of K' Save
1993Masumoto, A;Hemler, ME;Multiple activation states of VLA-4. Mechanistic differences between adhesion to CS1/fibronectin and to vascular cell adhesion molecule-1The Journal of biological chemistry7677996We examined the effects of a stimulatory anti-beta 1 mAb (TS2/16) and different divalent cations on VLA-4-mediated cell adhesion to vascular cell adhesion molecule-1 (VCAM-1), to the Save
1993Stauffer, P;Zhao, H;Luby-Phelps, K;Moss, R;Star, R;Muallem, S;Gap junction communication modulates [Ca2+]i oscillations and enzyme secretion in pancreatic acini.The Journal of biological chemistryThese oscillations stopped in allcells on stopping stimulationindependent of the number or the length of the focal stimulations. Global CCKJ stimulation of the same acinus also induced [ Save
1993Sinha, SK;Todd, SC;Hedrick, JA;Speiser, CL;Lambris, JD;Tsoukas, CD;Characterization of the EBV/C3d receptor on the human Jurkat T cell line: evidence for a novel transcriptJournal of immunology (Baltimore, Md. : 1950)8390533(28) demonstrated that HPB-ALLcells coexpress CR2 and CRl and that a portion of these two C3 receptors are associated in the form of CRl/CR2 complexes in the cell membrane. Save
1993Panzeter, PL;Ringer, DP;DNA precursors are channelled to nuclear matrix DNA replication sitesThe Biochemical journal8352746Precursors injected into the bloodstream do not have equal access to allcells, especially during the short pulse times used in this study. Many replicating cells will not see the precursor Save
1993D'Andrea, RJ;Stratmann, R;Lehner, CF;John, UP;Saint, R;The three rows gene of Drosophila melanogaster encodes a novel protein that is required for chromosome disjunction during mitosisMolecular biology of the cell8305737(I) An embryo during cellularization showing lack of transcripts in allcells except pole (presumptive germ) cells. 0) Cellular blastodern at higher magnification showingpunctate staining Save
1993Dorocicz, IR;Williams, PM;Redfield, RJ;The Haemophilus influenzae adenylate cyclase gene: cloning, sequence, and essential role in competenceJournal of bacteriology8226661This cAMP-independent event must be induced in allcells when cultures are transferred to MIV, but it may also occur spontaneously in the small fraction of cells that develop Save
1993Humm, J;Chin, L;A Model of Cell Inactivation by Alpha-Particle Internal EmittersRadiation ResearchThe bottom survival curve is produced by 50% of the sources bound uniformly to allcells. The middle curve is for 50% of the source decays occurring on half of the cells for type A Save
1993Mukhtar, DD;Stewart, IJ;Observations of the surface morphology of cultured granulated metrial gland cellsJournal of anatomy8226296The features described above were found on GMG cells at all stages of culture examined and on allcells from all stages of pregnancy examined. However, it was noticeable that the Save
1993Edelsbrunner, H;Seidel, R;Sharir, M;On the Zone Theorem for Hyperplane ArrangementsSIAM Journal on ComputingWe define Zone(b; H) to be the set of allcells in the arrangement A(H) that intersect the hypewlane b, and the cells were defined to be open sets. Previously the zone used to be defined Save
1993Ohno, H;Inoue, T;Akasaka, T;Okumura, A;Miyanishi, S;Ohashi, I;Kikuchi, M;Masuya, M;Amano, H;Imanaka, T;Acute lymphoblastic leukemia associated with a t(1;19)(q23;p13) in an adultInternal medicine (Tokyo, Japan)8286840ThePCRanalysis of the present patient revealed the samemolecular evidence of E2A/PBX1 fusion as found in childhood ALLcells. Thus, molecular analysis of the E2A/PBX1fusion gene Save
1993Herrmann, H;Karsch, F;LARGE SCALE COMPUTATIONAL PHYSICS ON MASSIVELY PARALLEL COMPUTERSLarge Scale Computational Physics on Massively Parallel Computerstime matrix vector multiplication 19.4 sec sum over allcells 0.012 sec Time for allcells to allcells communication. Save
1993Shamim, KM;Frog retinal ganglion cells. Morphological and spatial analysesThesisFor an auto-correlogram, allcells of the same type as the central reference cell are picked up (asshown schematically in A3) while a cross-correlogram locates all cellsof another type in Save
1992Kim, CY;Giaccia, AJ;Strulovici, B;Brown, JM;Differential expression of protein kinase C epsilon protein in lung cancer cell lines by ionising radiationBritish journal of cancer1329908The effect of ionising radiation on the regulation of gene and protein expression is complex. This study focuses on the translational regulational of the epsilon isoform of protein kinase C Save
1992Rooney, IA;Davies, A;Morgan, BP;Membrane attack complex (MAC)-mediated damage to spermatozoa: protection of the cells by the presence on their membranes of MAC inhibitory proteinsImmunology1374057On fluorescence microscopy, allcells stained brightly with both BRIC 229 and A 35 anti-CD59 antibodies (Fig. 2a-c). The pattern ofstaining vfaried (eg Fig. Save
1992Smedts, F;Ramaekers, F;Troyanovsky, S;Pruszczynski, M;Robben, H;Lane, B;Leigh, I;Plantema, F;Vooijs, P;Basal-cell keratins in cervical reserve cells and a comparison to their expression in cervical intraepithelial neoplasiaThe American journal of pathology1372156A completely filled bar indicates an intensely positive reaction in allcells. An open bar indicates that all cells are negative, whereas a small square indicates that only afew cells Save
1992Hohmann, HP;Remy, R;Aigner, L;Brockhaus, M;van Loon, AP;Protein kinases negatively affect nuclear factor-kappa B activation by tumor necrosis factor-alpha at two different stages in promyelocytic HL60 cellsThe Journal of biological chemistry1730737Allcells were used after reaching near confluence or a density of 1-1.5 X lo6 cells/ml of culture medium. Preparation of Nuclear Extracts and Electrophoretic Mobility Shift Assays (EMSA) Save
1992Kirby, MS;Sagara, Y;Gaa, S;Inesi, G;Lederer, WJ;Rogers, TB;Thapsigargin inhibits contraction and Ca2+ transient in cardiac cells by specific inhibition of the sarcoplasmic reticulum Ca2+ pumpThe Journal of biological chemistry1320000Recently, it has been reported that in allcells examined, a common response evoked by thapsigargin is a rapid increase in cytosolic [Ca2+],possibly resulting from the mobilization of Save
1992Bednarczyk, J;Szabo, M;McIntyre, B;Post-translational processing of the leukocyte integrin alpha 4 beta 1.Journal of Biological ChemistryEndo F digestion of a4 and B1 from HPB-ALLcells. and and IL-2-dependent T lymphocytes cultured for 43 days (Fig. HPB-ALL cells were 'ZsII-cell-surface-labeled lysed in Nonidet P4). Save
1992García-Alvarado, JS;Rodriguez, MA;Labbé, RG;Influence of elevated temperature on starch hydrolysis by enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens type AApplied and environmental microbiology1371660The suspension was sonicated for 45 s with 60-s cooling periods until essentially allcells were disrupted. The sample was thencentrifuged at 10,000 x g for 30 min, and Save
1992Szabà, G;Pine, PS;Weaver, JL;Kasari, M;Aszalos, A;Epitope mapping by photobleaching fluorescence resonance energy transfer measurements using a laser scanning microscope systemBiophysical journal1380319
1992Shatz, CJ;The developing brainScientific American26269896Allcells on the array fired with in about five seconds of one another, in a predictable and rhythmic pattern. The bursts of action potentials lasted several seconds and were followed by Save
1991Bejar, I;A GENERATIVE APPROACH TO PSYCHOLOGICAL AND EDUCATIONAL MEASUREMENTETS Research Report Seriesin this taxonomy could be assessed by the degree to which the psychometric parameters from a given cell are well-predicted and the within-cell residuais are constant across allcells. Save
1991Burton, GF;Kosco, MH;Szakal, AK;Tew, JG;Iccosomes and the secondary antibody responseImmunology1879876The resulting preparations were observed microscopically to ensure that allcells were disrupted. Antigen concentration was determined by using radiolabelled antigen and assessing Save
1991Hudson, TH;Grillo, FG;Brefeldin-A enhancement of ricin A-chain immunotoxins and blockade of intact ricin, modeccin, and abrinThe Journal of biological chemistry1917980This receptor interacts with transport of newly synthesized plasma membrane glycoproproteoglycans in allcells studied the Gal bindingsitesontheB-chain of ricin and would teins, Save
1991Novy, RE;Lin, JL;Lin, JJ;Characterization of cDNA clones encoding a human fibroblast caldesmon isoform and analysis of caldesmon expression in normal and transformed cellsThe Journal of biological chemistry1885618Allcells were grownin Dulbecco's modified Eagle's mediumcontaining 10% fetal calf serum and maintained in a 37 "C humidified incubator with 95% air and 5% CO,. Western Blot and Save
1991Järveläinen, HT;Kinsella, MG;Wight, TN;Sandell, LJ;Differential expression of small chondroitin/dermatan sulfate proteoglycans, PG-I/biglycan and PG-II/decorin, by vascular smooth muscle and endothelial cells in cultureThe Journal of biological chemistry1744124
1991van Praag, MC;Boom, BW;van Hees, CL;Vermeer, BJ;Scheffer, E;Classical and ulcerative lichen planus with plasma cell infiltrateArchives of dermatology1990998Plasma cells accounted for 60% to 70% of allcells. The infiltrate invaded the epidermis causing apoptoticand vacuolar degeneration of basal and parabasal cells (Fig 2). The lesions of Save
1991Humbert, P;Agache, P;Acquired ichthyosis: a new cutaneous marker of autoimmunityArchives of dermatology1990997Plasma cells accounted for 60% to 70% of allcells. The infiltrate invaded the epidermis causing apoptoticand vacuolar degeneration of basal and parabasal cells (Fig 2). The lesions of Save
1991Hall, AJ;Steady size distributions in cell populations : a thesis presented in partial fulfilment of the requirements for the degree of Doctor Philosophy in Mathematics at Massey UniversityThesisSpecial cases are also considered where allcells with the same birth-size divide at the ), and allcells divide into equal-sized daughters, then we obtain a functional differential equation. Save
1990Finn, PW;Kara, CJ;Van, TT;Douhan, J;Boothby, MR;Glimcher, LH;The presence of a DNA binding complex correlates with E beta class II MHC gene expressionThe EMBO journal2328725Even though the three motifs do not enhance CAT activity when tested individually, allcells that we have tested contain factors that bind to W, X and Y (Figure 4). Save
1990Yayon, A;Klagsbrun, M;Autocrine transformation by chimeric signal peptide-basic fibroblast growth factor: reversal by suraminProceedings of the National Academy of Sciences of the United States of America2164679Most if not allcells possess FGF receptors, which are phosphorylated andinternalized upon binding of FGF. A 130-kDa high-affinity FGF receptor containing an Save
1990Berger, JD;Rahemtullah, S;Commitment to autogamy in Paramecium blocks mating reactivity: implications for regulation of the sexual pathway and the breeding systemExperimental cell research2298253From this point on, allcells become committed to autogamy at a cell density that is lover than that required for the onset of mating reactivity. The cell cycle experiments discussed above Save
1990Dixon, CJ;Woods, NM;Cuthbertson, KS;Cobbold, PH;Evidence for two Ca2(+)-mobilizing purinoceptors on rat hepatocytesThe Biochemical journal2386488Other than in the total absence of receptors of both subsets, allcells will respond to ATP.We propose that the cells responding to ATP with transients of short duration lack receptors of Save
1989Malinow, R;Schulman, H;Tsien, RW;Inhibition of postsynaptic PKC or CaMKII blocks induction but not expression of LTPScience (New York, N.Y.)2549638Bath-applied H-7 can suppress the expression of LTP (3), although it is not known whether the locus of action is pre- or postsynaptic, since it has access to allcells in the preparation. To Save
1989Johnson, JP;Stade, BG;Holzmann, B;Schwäble, W;Riethmüller, G;De novo expression of intercellular-adhesion molecule 1 in melanoma correlates with increased risk of metastasisProceedings of the National Academy of Sciences of the United States of America2643120Identical restriction fragments were observed in allcells analyzed, including four autologous melanoma/B-cell pairs. The P3.58/ICAM-1 gene, therefore, does not seem to be polymorphic Save
1989Knust, B;Brüggemann, U;Doerfler, W;Reactivation of a methylation-silenced gene in adenovirus-transformed cells by 5-azacytidine or by E1A trans activationJournal of virology2473219Of course, it was not possible to show that allcells had been transfected equally efficiently. Reactivation of E2Atranscription might have occurred only in a subpopulation of the HE3 cells. Save
1989Celis, E;Karr, RW;Presentation of an immunodominant T-cell epitope of hepatitis B surface antigen by the HLA-DPw4 moleculeJournal of virology2463381Thus, it appears that in the heterogeneous PBMC population not allcells express DPw4 molecules and that only thosethat do can present HBsAg (or Sld) to the Th. The inhibitory effect Save
1989Navaratnam, V;Woodward, JM;Skepper, JN;Specific heart granules and natriuretic peptide in the developing myocardium of fetal and neonatal rats and hamstersJournal of anatomy2532637Within the fetal atria, almost allcells seem capable of ANP synthesis and the concentration of ANP immunoreactivity increases sharply with development even after birth. Recent light Save
1988Nicolson, GL;Dulski, KM;Trosko, JE;Loss of intercellular junctional communication correlates with metastatic potential in mammary adenocarcinoma cellsProceedings of the National Academy of Sciences of the United States of America3422438, and the gene coding for polyoma virus middlesized tumor (middle T) antigen can decrease intercellular junctional communication in certain cellsystems (13-16), although not allcells Save
1988Hall, JG;Review and hypotheses: somatic mosaicism: observations related to clinical geneticsAmerican journal of human genetics3052049Conditions such as McCune-Albright syndrome may well represent lethal human mutations when present in allcells, but, by isolating the tissue involved, it may be possible to compare Save
1988Siebenlist, U;Bressler, P;Kelly, K;Two distinct mechanisms of transcriptional control operate on c-myc during differentiation of HL60 cellsMolecular and cellular biology3162550These sites occur in allcells which we have analyzed (46, 47). In previous reports (3, 20, 22, 28, 29), only partial and even somewhat conflicting information on the chromatin structure of Save
1988Adány, R;Glukhova, MA;Kabakov, AY;Muszbek, L;Characterisation of connective tissue cells containing factor XIII subunit aJournal of clinical pathology2893813Though the expression of certain antigens might be lost at culture, allcells stained for the fibroblast markers IIIG1O and alkaline phosphatase lack this antigen. This clearly suggests that Save
1988Kim, T;Zeigler, B;The class kernel-models in DEVS-scheme: a hypercube architecture exampleACM SIGSIM Simulation Digestset-allcells-positions, which generates 2 n number of n-tuple coordinates to be attached to corresponding components before locating them in their positions. The method set-allcells- Save
1988Reno, PW;Immunochemical Approaches to Coastal, Estuarine and Oceanographic QuestionsCoastal and EstuarineN ear ly identic al reactions were found in kidney (Figure 2?) and liver (data not shown) -allcells seeme d to conta in IPN antige n. However, gastrointestin al tra ct tissue, especiallyt he Save
1987Art, JJ;Fettiplace, R;Variation of membrane properties in hair cells isolated from the turtle cochleaThe Journal of physiology2443666The shape of the current-voltage relationship was found to be similar in allcells, being flat at hyperpolarized levels and increasing its slope with depolarization through the resting Save
1987Segarini, PR;Roberts, AB;Rosen, DM;Seyedin, SM;Membrane binding characteristics of two forms of transforming growth factor-betaThe Journal of biological chemistry2889729Essentially allcells have high affinity receptors that specifically bind TGF-/3 (Kdvalues of 1-60 pM, Refs. 11, 12, 14, and 15). The receptor number is tissue- and species-specific and Save
1987Richardson, A;Butler, JA;Rutherford, MS;Semsei, I;Gu, MZ;Fernandes, G;Chiang, WH;Effect of age and dietary restriction on the expression of alpha 2u-globulinThe Journal of biological chemistry2442168regulation of gene expression represents one site regulation of common to allcells in a living organism, and changes in gene 24. expression can have profound effect at both the a Save
1987ROBERTS, D;A zonal method for modeling 3-D aircraft flow fields with jet plume effects19th AIAA, Fluid Dynamics, Plasma Dynamics, and Lasers ConferenceCON is then updated, and the cycle 18 repeated until the error in continuity falls below a prescribed tolerance for allcells . The relaxation factor, 0, Save
1986Greaves, MF;Differentiation-linked leukemogenesis in lymphocytesScience (New York, N.Y.)3535067us the monodonal antibody-defined makers T9 and T10, described initially on T-ALLcells and normal thymocyte subses (21), are not T lineage or maturation stage restrictedbut are Save
1986Mann, GJ;Fox, RM;Deoxyadenosine triphosphate as a mediator of deoxyguanosine toxicity in cultured T lymphoblastsThe Journal of clinical investigation3490493Allcells were maintained at 37C in suspension culture in Hepes-buffered Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% fetal bovine serum, glutamine (2 Save
1986Slater, LM;Sweet, P;Stupecky, M;Gupta, S;Cyclosporin A reverses vincristine and daunorubicin resistance in acute lymphatic leukemia in vitroThe Journal of clinical investigation3457021It has recently been reported that human ALLcells have a 10-fold greater concentration of calmodulin than normal peripheral blood lymphocytes and that CsA is a potentinhibitor of T Save
1986Cowley, GP;Smith, JA;Gusterson, BA;Increased EGF receptors on human squamous carcinoma cell linesBritish journal of cancer2420349The binding kinetics for allcells examined showed the typical pattern of a high and low affinity receptor with dissociation constants of 10-10M and 1-9M respectively. Recent Save
1986Stewart, MA;Warnock, M;Wheeler, A;Wilkie, N;Mullins, JI;Onions, DE;Neil, JC;Nucleotide sequences of a feline leukemia virus subgroup A envelope gene and long terminal repeat and evidence for the recombinational origin of subgroup B virusesJournal of virology3009890FEA cells (21) were infected in mass culture with various FeLV isolates and passaged for 3 weeks to allow virus spread to allcells. DNA was purified from these cell cultures for use in Save
1986DiFrancesco, D;Ferroni, A;Mazzanti, M;Tromba, C;Properties of the hyperpolarizing-activated current (if) in cells isolated from the rabbit sino-atrial nodeThe Journal of physiology2432247The reason for this discrepancy may be the current run-down that occurs, although to a highly variable degree, in allcells. This feature of if is treated insome detail below. Here it may be Save
1986Hartman, BJ;Tomasz, A;Expression of methicillin resistance in heterogeneous strains of Staphylococcus aureusAntimicrobial agents and chemotherapy3488015explanation of heterogeneity is that allcells in the heterogeneous culture carry the genetic marker(s) of methicillin resistance, but for some reason the phenotypic expression of Save
1986Wang, CY;Bushkin, Y;Pica, R;Lane, C;McGrath, H;Posnett, DN;Stimulation and expansion of a human T-cell subpopulation by a monoclonal antibody to T-cell receptor moleculeHybridoma2429909Both the aand (3-chains from normal PBL-T blasts resolved in the pi range of 5.5-6.5, whereas the a- and /3-chains from HPB-ALLcells had acidic and basic pis of 4.0 and 7.0-8.5, Save
1986Steiness, E;Positive inotropic drugs--digitalisActa medica Scandinavica. Supplementum3017057Digitalis is known to inhibit the Na+K+-ATPase in allcells in nearly all species. It has been aqyed that this effect induces increase of the inuacellular sodium concentnfion within the Save
1985Lehrer, RI;Daher, K;Ganz, T;Selsted, ME;Direct inactivation of viruses by MCP-1 and MCP-2, natural peptide antibiotics from rabbit leukocytesJournal of virology2985808Allcells used in this manner were obtained from the American Type Culture Collection except for MDBK cells, which were a gift from Debi Nayak. The virus-infected cell monolayers Save
1985Morris, CS;Hitchcock, E;Immunocytochemistry of folliculo-stellate cells of normal and neoplastic human pituitary glandJournal of clinical pathology3889065folliculo-stellate cells stain for GFAP and S100, although not allcells stained for S100 are GFAP positive. Cocchia and Miani" showed S100 protein within the stellate cells of the pars Save
1985Neyses, L;Locher, R;Stimpel, M;Streuli, R;Vetter, W;Stereospecific modulation of the calcium channel in human erythrocytes by cholesterol and its oxidized derivativesThe Biochemical journal2581557Furthermore, allcells were energized to the same extent by ATP (about 1.7mM). Since the anion channel may be influenced by cholesterol (Schubert & Boss, 1982), we checked Save
1985Hueston, WD;Herd summaries of individual cow somatic cell counts and mastitis control researchThesisand th e re fo re abnormal; 2) allcells in milk were desquamated se c re to ry cells of epithelialo rig in , and th e re fo re a normal p art of la c to genes is ; and 3) the cells in normal milk Save
1984Buick, RN;Pollak, MN;Perspectives on clonogenic tumor cells, stem cells, and oncogenesCancer research6386145In prokaryotes, control of proliferation is unrelated to the process of differentiation, and allcells have proliferative potential. As we have shown, this is not the case in tissues of higher Save
1984Gaukroger, JM;Wilson, L;Protection of cells from methotrexate toxicity by 7-hydroxymethotrexateBritish journal of cancer6590087Cell growth survival studies have revealed that 7-OH methotrexate is two orders of magnitude less cytotoxic to human melanoma and human acute lymphoblastic leukaemia (ALL) cells Save
1984Gussander, E;Adams, A;Electron microscopic evidence for replication of circular Epstein-Barr virus genomes in latently infected Raji cellsJournal of virology6092676Circular forms, which can be isolated either in the covalently closed, supercoiled structure or as a relaxed, open DNA circle (16), have subsequently been found in allcells with more Save
1984Epstein, LM;Forney, JD;Mendelian and non-mendelian mutations affecting surface antigen expression in Paramecium tetraureliaMolecular and cellular biology6092921A genetic and molecular analysis of this mutant suggests that although the A gene is present in the micronucleus of allcells, it is lost during the formation of the macronucleus in the Save
1984Yinon, U;Myopia induction in animals following alteration of the visual input during development: a reviewCurrent eye research6713963For example, asfar as has been determined, allcells of the salamander are capable of ded if fe rent iat ion with appropriate stimulation. Extensive in vitro experimentation on amphibian Save
1984Nelson, DO;Graham, CA;Effects of development and captopril treatment on central angiotensin II--sensitive neuronsClinical and experimental hypertension. Part A, Theory and practice6398145Nearly allcells examined, including neurons which were relatively insensitive to A11 or saralasin, were potently ex
1983Overath, P;Czichos, J;Stock, U;Nonnengaesser, C;Repression of glycoprotein synthesis and release of surface coat during transformation of Trypanosoma bruceiThe EMBO journal6688990Six hours after transfer of the cells to medium SDM-79, ie, at a time when VSG synthesis has already ceased, allcells retain the coat (compare sample at t= 6 h with freshly harvested Save
1983Rowin, KS;Tanowitz, HB;Wittner, M;Nguyen, HT;Nadal-Ginard, B;Inhibition of muscle differentiation by trypanosoma cruziProceedings of the National Academy of Sciences of the United States of America6413976Normal quantities of 13 and y-actin were present in allcells. (B) Rehybridization of the RNA blot shown in A with 32P-labeled DNA withnonmuscle-specific sequencescoding Save
1983Meo, T;Gramsch, C;Inan, R;Höllt, V;Weber, E;Herz, A;Riethmüller, G;Monoclonal antibody to the message sequence Tyr-Gly-Gly-Phe of opioid peptides exhibits the specificity requirements of mammalian opioid receptorsProceedings of the National Academy of Sciences of the United States of America6191329Allcells in the intermediate lobe of rat pituitary were intensely stained (Fig. 3). Higher magnification revealed heavy cytoplasmic localization of the immunoreactive peptides. Save
1983Otteskog, P;Sundqvist, KG;Anchorage and lymphocyte function. Spreading-capacity distinguishes common thymocytes and peripheral T lymphocytesImmunology6339376T-enriched human blood lymphocytes with an adhesive surface in the presence of Concanavalin A (Con A) almost immediately induced a sequence of motilechanges in virtually allcells Save
1983O'NEIL, PM;Log-linear models for evaluating hunting demandThesisBoth the Pearson ch i-sq ua re statistic and th e lik e lih oodratio sum over allcells in th e ta b le . Both a re asymp to tic a lly d is tribu te d as chi - square . Save
1983Burman, LG;Raichler, J;Park, JT;Evidence for diffuse growth of the cylindrical portion of the Escherichia coli murein sacculusJournal of bacteriology6350274The lengths of allcells that contained grains and the distances of the grains from the nearest cell pole were measured with a ruler. Forplotting, these distances were expressed as Save
1982Marx, JL;Cloning the genes of the MHCScience (New York, N.Y.)7071587These regions code not for transplantation antigens, which are expressed on allcells, but for differentiation antigens that are foundonly on certain types of immune cells. The differ Save
1982Zingg, HH;Patel, YC;Biosynthesis of immunoreactive somatostatin by hypothalamic neurons in cultureThe Journal of clinical investigation7130395Immediately after being plated, allcells exhibited a uniform round-shaped appearance and were evenly distributed on the plate surface. After day 5 in culture, many cells showed a Save
1982Donta, ST;Sullivan, N;Wilkins, TD;Differential effects of Clostridium difficile toxins on tissue-cultured cellsJournal of clinical microbiology7107845The two toxins' effects are not similar on allcells, however, since they produce different Allcells were tested at the same time with identical toxin preparations. b ++, All cells rounded Save
1982Ueda, R;Tanimoto, M;Takahashi, T;Ogata, S;Nishida, K;Namikawa, R;Nishizuka, Y;Ota, K;Serological analysis of cell surface antigens of null cell acute lymphocytic leukemia by mouse monoclonal antibodiesProceedings of the National Academy of Sciences of the United States of America6956869Production of Hybridomas Against063 Null-ALLCells. ALL cells wereobtained fromtheperipheralblood ofa22-yearold female patient. The surface phenotypeof the leukemia cells Save
1982Cochrane, DE;Distel, DL;Association of 45calcium with rat mast cells stimulated by 48/80: effects of inactivation, calcium and metabolic inhibitionThe Journal of physiology6816921Mast cells were stimulated by48/80 (1 4g/ml.) and unlabelled Ca (1 mM) added to allcells 10 sec later. Atthe indicated times afterward, 4"Ca (50 #sC/ml.)was added and the incubation Save
1982Ayres, K;Sklar, R;Larson, K;Lindgren, V;Strauss, B;Regulation of the capacity for O6-methylguanine removal from DNA in human lymphoblastoid cells studied by cell hybridizationMolecular and cellular biology6897101so that the dose shown is that which would be observed if allcells had the reactivity of ROT-5. Symbols: A, ROT-5; 0, L6; O, hybrid clone no. 2; E, hybrid clone no. Save
1982Little, LM;Shadduck, JA;Pathogenesis of rotavirus infection in miceInfection and immunity6292110It should be appreciated that in the current study not allcells within any anatomical region fluoresced. Rather, fluorescing cells normally occupied less than 10%, up to perhaps 50%, of Save
1982山田和義, ;岡部直衛, ;宇塚善郎, ;FAB 分類における成人急性白血病の走査電顕及び表面マーカーによる解析臨床血液Almost allcells have ridge-like pro丘1es.(×5,800) Almost allcells are positive for both ANE and CAEin the same cell. b:Almost allcells have ridge-1ike pro丘Ies or microvilli・(×3,000) C: Save
1981Abbott, A;¡Graphs, Not Punctuation!ScienceIndeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save
1981Libet, B;" Human Life" TestimonyScience7244627Indeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save
1981Osborn, EF;Hubbert's EstimatesScience (New York, N.Y.)17782756Indeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save
1981Hamp, EP;;Graphs, not punctuation!Science (New York, N.Y.)17782755Indeed, given suitable technology, it is theoretically possiblefor many if not allcells in the adult human body each to be clonedinto an entirely new human individual. Should-we confer Save
1981Engleman, EG;Warnke, R;Fox, RI;Dilley, J;Benike, CJ;Levy, R;Studies of a human T lymphocyte antigen recognized by a monoclonal antibodyProceedings of the National Academy of Sciences of the United States of America7015346A second screening identified a subgroup of these clones whose antibody products bound equally to normal peripheralTcells andto the immunizing T-ALLcells. The binding specificity of Save
1981Yokoyama, K;Stockert, E;Old, LJ;Nathenson, SG;Structural comparisons of TL antigens derived from normal and leukemia cells of Tl+ and TL- strains and relationship to genetically linked H-2 major histocompatibility complex productsProceedings of the National Academy of Sciences of the United States of America6947273H-2 antigens are expressedon virtually allcells ofthe body, whereas TL antigensare normally restrictedto thymocytes in TL+ strains. In contrast to the remarkable polymorphism of H-2 (10 Save
1981Hanania, N;Shaool, D;Poncy, C;Harel, J;Manifold expression of new cellular genes in human lymphoid neoplasiaProceedings of the National Academy of Sciences of the United States of America6947241Previous studies performed in our laboratory have shown that a minor single-stranded DNA component (ssDNA) isolated from the bulk of double-stranded nuclear DNA (nDNA) of Save
1981Mechler, B;Rabbitts, TH;Membrane-bound ribosomes of myeloma cells. IV. mRNA complexity of free and membrane-bound polysomesThe Journal of cell biology6782109Allcells were harvested during exponentialgrowth from cultures not exceeding 5.0 x 106 cells/ml. When labeling was required, [ 3H]uridine, [3Madenosine, or [ 3H]oleic acid wasadded Save
1981Knipe, DM;Batterson, W;Nosal, C;Roizman, B;Buchan, A;Molecular genetics of herpes simplex virus. VI. Characterization of a temperature-sensitive mutant defective in the expression of all early viral gene productsJournal of virology6264126However, the yield of syn+ virus was approximately 10% of the yield of the syn virus, even though allcells were coinfected with both. Therefore, even the wild-type parent of tsB7 could Save
1981Grover, NB;Margalit, A;Zaritsky, A;Ben-Hur, E;Hansen, MT;Sensitivity of exponentially growing populations of Escherichia coli to photo-induced psoralen-DNA interstrand crosslinksBiophysical journal7023563
1981Zaritsky, A;Macnab, RM;Effects of lipophilic cations on motility and other physiological properties of Bacillus subtilisJournal of bacteriology6792185The effects of TPAs+ on the motor were readily reversible: 20 min after treatment with 50 ,uM TPAs+, cells were filtered, washed, and suspended in chemotaxis buffer; 3 minlater, allcells Save
1981Lindgren, V;The location of chromosome breaks in Bloom's syndromeCytogenetics and cell genetics7471822Average of samples IB and 2 only; when allcells are included, the average is 50.7%. Table II. Distribution of breaks in late-non-late-replicating areas1 Total Save
1981Carpio, M;Bellamy, JE;Babiuk, LA;Comparative virulence of different bovine rotavirus isolatesCanadian journal of comparative medicine : Revue canadienne de medecine comparee6268264Twenty-four hours postinfection, when allcells were detached from the monolayers, the entire culture fluids were sonicated to release intracellular virus. The virus was pelleted through a Save
1981Thaller, RA;Potential use of 52FE porphyrins as tumor scanning agentsThesisHowever in vivo tumor killing was not complete resulting in many smaller tumors due to the factthat not allcells were exposed to the light or took up the hematoporphyrin. (220) Over 300 Save
1980O'Lague, PH;Huttner, SL;Physiological and morphological studies of rat pheochromocytoma cells (PC12) chemically fused and grown in cultureProceedings of the National Academy of Sciences of the United States of America6929516In allcells regardless of growth conditions membrane resistance decreased with depolarization. Fig. 1C shows superimposed voltage responses to one depolarizing and one hyperpo Save
1980Lasky, LA;Lev, Z;Xin, JH;Britten, RJ;Davidson, EH;Messenger RNA prevalence in sea urchin embryos measured with cloned cDNAsProceedings of the National Academy of Sciences of the United States of America6254074per average cell given on the lower abscissas are minimal estimates calculated on the basis that there are 600 cells per gastrula and 1500 cells per pluteus embryo and that allcells are Save
1980Haas, M;Patch, V;Genomic masking and rescue of dual-tropic murine leukemia viruses: role of pseudotype virions in viral lymphomagenesisJournal of virology6252327Allcells were grown in Dulbecco-Vogt modified Eagle minimum essential medium supplemented with 10%o fetal bovine serum. All cells were split routinelyat a ratio of 1:16 upon Save
1980Weller, SK;Joy, AE;Temin, HM;Correlation between cell killing and massive second-round superinfection by members of some subgroups of avian leukosis virusJournal of virology6245246this study were determined by interference assays as follows: chicken cells in 100-mm petri dishes were infected with different avian leukosis viruses and passaged twice so that allcells Save
1980Berg, JM;Gardner, HA;Gardner, RJ;Goh, EG;Markovic, VD;Simpson, NE;Worton, RG;Dic(21;21) in a Down's syndrome child with an unusual chromosome 9 variant in the motherJournal of medical genetics6445984In allcells analysed, the translocation chromosome was of the same relative size and banding pattern, indicating a high degree of stability. No short structures or chromosome fragments Save
1980Gerrard, JM;Clawson, CC;White, JG;Lysophosphatidic acids: III. Enhancement of neutrophil chemotaxisThe American journal of pathology7416233Figure 2-When 0.35 ,ug FMP/ml was added to the incubation media used in Figure 1, marked changes were seen in allcells. There were large projections from the body of the cell, and Save
1980Brown, AT;Breeding, LC;Carbon dioxide metabolism by Actinomyces viscosus: pathways for succinate and aspartate productionInfection and immunity6769822Allcells were washed once with 0.01 M potassium phosphate buffer, pH 7.0, and the cell pellets were frozen at -20'C until used. Laboratory cultures of A. viscosus were stored as Save
1980Koppes, LJ;Nanninga, N;Positive correlation between size at initiation of chromosome replication in Escherichia coli and size at initiation of cell constrictionJournal of bacteriology6995452We have also assumed equal growth rate constants for allcells. Variation of the growth rate will tend to decrease the correlation. However, the CV of the specific growth rate is probably Save
1980Ho, CC;Interaction studies of ethanol and psychotropic drugs in rodents: possible involvement of adenosine triphosphatasesThesisD evelopm ent of th e Sodium Pump C oncept T here is ad iffe re nce in io n ic concen tra tio n betw een intracellular and ex tr ace llu la rf lu id s in allcells . Save
1979Detwiler, PB;Hodgkin, AL;Electrical coupling between cones in turtle retinaThe Journal of physiology225478If it is assumed that allcells have the same total membrane resistance, rm, but that different pairs differ in the amount of coupling, then this method of plotting should bring all the points Save
1978Mukherjee, AB;Orloff, S;Butler, JD;Triche, T;Lalley, P;Schulman, JD;Entrapment of metaphase chromosomes into phospholipid vesicles (lipochromosomes): carrier potential in gene transferProceedings of the National Academy of Sciences of the United States of America349565Allcells were checked for mycoplasma (10) and foundto be free of those pathogens. These hybrid cells contributed the metaphase chromosomes which were used to prepare the Save
1978Esber, EC;Movassaghi, N;Leikin, SL;Surface membrane determinants on childhood acute lymphocytic leukemia cells: immunoglobulin, Fc and C3 receptorsClinical and experimental immunology278686Surface membrane determinants on ALLcells Other investigators have found Fc receptors on ALLcells, in combination with either E-rosette formation or with C3 receptors (Haegert et al., Save
1977Thoene, JG;Oshima, RG;Ritchie, DG;Schneider, JA;Cystinotic fibroblasts accumulate cystine from intracellular protein degradationProceedings of the National Academy of Sciences of the United States of America270698After 20 hr, allcells were depleted of intracellular cystine bytreatment with MEA for 1 hr,and then placed in cystine-deficientmedium. Duplicate plates were harvested-at the times shown Save
1977Basset, F;Soler, P;Jaurand, MC;Bignon, J;Ultrastructural examination of broncho-alveolar lavage for diagnosis of pulmonary histiocytosis X: Preliminary report on 4 casesThorax302040and 2) in great numbers in case t, amounting to 7% of allcells examined. In the three other patients, HX cells were much less numerous, but their presence was still diagnostic. In all four Save
1977Genther, CS;Schoeny, RS;Loper, JC;Smith, CC;Mutagenic studies of folic acid antagonistsAntimicrobial agents and chemotherapy329758Since the folic acid pathway is common to allcells, the mechanisms operative with S. faecium will probably be similar in other types of organismssusceptible to these compounds. When Save
1977Rubenstein, M;The effect of salicylate on macromolecular synthesis in mammalian and bacterial cells.ThesisThe inhibition obtained in the growth experiment applied to allcells involved and did not select for a small population of cellswhile drastically inhibiting the remainder. Save
1977Tso-Kai Liu, ;Synthesis of Multilevel Feed-Forward MOS NetworksIEEE Transactions on ComputersTheorem 1: There always exists a feed-forward MOS network with a minimum number ofcells, realizing a given function f such that the output functions of allcells in the network are Save
1976Bootello, A;Fernandez-Cruz, E;Escartin, P;Blanco, MF;Gosalvez, M;Segovia De Arana, JM;Injurious effect on rat liver mitochondria by lymphocytes from patients with primary biliary cirrhosisClinical and experimental immunology1277585The antibody is non-organ/non-species-specific and reacts with allcells containing mitochondria. The antigen is a lipoprotein localized in the inner mitochondrial membranes (Berg et al. Save
1976Gilligan, T;Patterson, A;High field conductance of some acids, bases, and saltsThe Journal of Physical ChemistryParticular care was taken to determine and follow the cell constants of allcells by the method of Jones and Bradshaw.11 It has been found that the cell constants of cells made with Save
1976Malek, KW;A METHOD FOR THE CULTIVATION OF DISPERSED EPITHELIAL CELLS FROM MOUSE PALATAL MUCOSA AND THE EFFECTS OF X-IRRADIATION.Thesislted in the release of cells from the ba sa l layer and a ls o from the lower layers of the stratum spinosum, whereas 30 minutes of incubation resulted in the d isp er sio n of almost allcells Save
1975Hyde, GM;MILK YIELD AS A FACTOR IN DAIRY HERD MANAGEMENT BY EXCEPTION.ThesisTable 4 gives regression coefficients from the analyses over allcells, by parity, by age group, and by season of calving. Calculatedpeak times of lactation are also tabulated. Note that Save
1975McKEE, RH;THE CONTRIBUTION OF SINGLE-STRAND BREAKS AND ALKALI-LABILE BONDS TO THE LOSS OF INFECTIVITY OF GAMMA-IRRADIATED PHI-X174 REPLICATIVE FORM DNA IN E. COLI CELLS MUTANT IN VARIOUS REPAIR FUNCTIONSThesisTwenty-one radiation sensitive mutants have been examined for their capacity to support y-irradiated 0X174 RF-DNA. The survival of 0X174 RF-DNA was reduced in essentially all of Save
1975Fiske, RA;Klein, PA;Effect of immunosuppression on the genetic resistance of A2G mice to neurovirulent influenza virusInfection and immunity163800This putative replication defect, however, must be highly selective, ie, must occur in some but not allcells of the A2G mouse, since WSN can undergo complete cycles of replication in Save
1975Goldhammer, L;ATS-6 Solar Cell Flight Experiment After 325 Days in Synchronous OrbitIEEE Transactions on Aerospace and Electronic SystemsAllcells performed well through 325 days in orbit, except the FEP-covered cells, which appear to have increased their rate of degradation during the first eclipse season. Save
1975Schriber, S;Comparison of standing wave accelerators operating in the 2π/3 and π/2 modesIEEE Transactions on Nuclear ScienceThe singly periodic (allcells identical) 23 cell system used for experimental measurements of relative axial electric field amplitudes hadthe following mean parameters; Save
1974Baker, MA;Ramachandar, K;Taub, RN;Specificity of heteroantisera to human acute leukemia-associated antigensThe Journal of clinical investigation4140196Similarly, antisera to ALLcells absorbed with AML cells retained significant cytotoxicity only to ALL cells. Control antisera raised in nontolerant mice were cytotoxic to all cells tested. Save
1974Rutishauser, U;Yahara, I;Edelman, GM;Morphology, motility, and surface behavior of lymphocytes bound to nylon fibersProceedings of the National Academy of Sciences of the United States of America4545428Allcells were firmly attached to the fibers, did not detach even after incubation for 4 hr, and in no case was translocation of a cell along the fiber observed. A small number of cells nonspe Save
1974Stella, JP;Yankaskas, KD;Morgan, JH;Fox, MP;Pfau, CJ;Characteristics of the in vitro inhibition of arenavirus synthesis by bis-benzimidazolesAntimicrobial agents and chemotherapy4451346After an additional 24 h, allcells appeared to have normal morphologies, but the reticule counts from drug-treated cultures were 10% below those of the controls. Moreover, after Save
1974VAIL, AF;DROPOUT FROM PSYCHOTHERAPY AS RELATED TO PATIENT-THERAPIST DISCREPANCIES,THERAPIST CHARACTERISTICS, AND INTERACTION IN RACE AND SEXThesisThis could be co rre c te dfor by tran sfo rm in g th e num ber of rem ainers in allcells , as ifallcells had the same total of dropouts and rem ainers. I t was arbitrarily decided to treatall the Save
1974Maccallum, RC;A COMPARISON OF TWO INDIVIDUAL DIFFERENCES MODELS FOR MULTIDIMENSIONAL SCALING: CARROLL AND CHANG'S INDSCAL AND TUCKER'S THREE-MODE FACTOR ANALYSISThesisDue to high costs of generating replications of data sets to fit thisdesign, and then analyzing the data, it was not possible to represent allcells of Table 3 in the present study. Only the Save
1974Johnson, BS;IMITATION BY CHILDREN OF MODEL-PERFORMED BEHAVIOR UNDER A VARIETY OF STIMULUS CONDITIONS.ThesisA test of the equality of regression within allcells was performed, and the hypothesis of differentialwithin cell regression was not supported (F = .62; Save
1974VON BERGEN JR, CW;Individual differences variables and performance goals as determinants of level of performance.ThesisTo accomplish this a procedure was employed which essentially involved equating the n Ach scores for allcells ofthe self-esteem analysisandthe locusof Save
1974Burnstein, T;Jacobsen, LB;Zeman, W;Chen, TT;Persistent infection of BSC-1 cells by defective measles virus derived from subacute sclerosing panencephalitisInfection and immunity4215764Fluorescent measles antigen was present in allcells. Co-cultures of IP-3-Ca cells with normal BSC-1 cells developed massive syncytia and extensive destruction. The IP-3-Ca cells Save
1974Katsura, Y;Uesaka, I;Assessment of germ tube dispersion activity of serum from experimental candidiasis: a new procedure for serodiagnosisInfection and immunity4207514Allcells in the suspension were yeast form, with a few paired, but no germ tubes or pseudohyphae were found. Intraperitoneal (ip) infection was made by inoculating 0.5 ml of the Save
1974Heilmann, LJ;Physical studies of unfolded 30S ribosomal subunits of Escherichia coliThesisE ar ly work establishedthat th eseparticles were ap parentlyub iq u it ous in allcells and consisted of ap p ro x imately eq ualproportions of RNA and protein. E ar ly pre parations# called Save
1974Bendix, ME;A study of the adenyl cyclase activity in testis of maturing chinook salmon (Oncorhynchus tshawytscha)ThesisWithin each cyst allcells are at the same stage of spermatogenic development and are maturing at the same rate but within a testis the various cysts may be at different stages of Save
1973Warburton, D;Henderson, AS;Shapiro, LR;Hsu, LY;A stable human dicentric chromosome, t dic (12;14)(p13;p13) including an intercalary satellite region between centromeresAmerican journal of human genetics4716663Allcells from a peripheral blood culture had 45 chromosomes, with a missing D and C group chromosome and an abnormal large submetacentric chromosome with a prominent Save
1973BERNS, A;AYAD, S;Intergeneric Transformation in Bacillus subtilisBiochemical Society Transactionsallcells may be potentialrecipientsof fore ig n MA? as a consequence of th euniversalityof th egenet ic code - on thisplanetatleast*In many ca se s? up ta ke and " in tegration " offore ig n Save
1973Dadds, MJS;Detection and survival of Zymomonas in breweriesThesisMethods were developed for detecting zymomonads in beer and brewing materials to and the microbiological quality control of beer and to investigate the incidence and survival of Save
1972Eaton, DC;Potassium ion accumulation near a pace-making cell of AplysiaThe Journal of physiology5071402If it is assumed that the real capacitance of a small unit area of membrane is the same for allcells in Aplysia, then capacitances larger than expected represent an underestimation of the Save
1972Munthe, E;Natvig, JB;Immunglobulin classes, subclasses and complexes of IgG rheumatoid factor in rheumatoid plasma cellsClinical and experimental immunology4563335Allcells binding RB200 conjugate fixed also the FITC conjugate in the second step because of binding either to the plasma cell rheumatoid factor Ig or to the added RB200-labelled Save
1972Sander, G;Jacobs, JP;Observations on interactions between fluorescein-iso-thiocyanate conjugates and cells in vitroJournal of clinical pathology4114699At each passage allcells were treated in an identical manner. The methods employed were as described in a previous communication by Sander (1969) and Sander et al (1970). The Save
1972Penn, RF;An experimental study involving the use of contract evaluation in a chem study and in a traditional high school chemistry courseThesisTEST OF EQUALITY OFREGRESSION IN ALLCELLS Save
1972Robertson, A;Cohen, MH;Control of developing fieldsAnnual review of biophysics and bioengineering4405416Wolpert's definition, "a field is that region in which allcells are having their positional information specified with respect to the same set of points," is the most useful one for our purposes, Save
1971Blankley, WF;Auxotrophic and heterotrophic growth and calcification in coccolithophoridsThesisAllcells retained a complete coccolith cover throughout the experiment(see also Fig. IV.?). Allcells retained a complete coccolith cover throughout the experiment. Save
1971BIEGEN, DA;The effects of irrelevant and immaterial data on problem difficultyThesisId ea lly , th is 'coefficient ' would be app lica b le w ith in allcells . Save
1971Jacobs, NJ;Jacobs, JM;Brent, P;Characterization of the late steps of microbial heme synthesis: conversion of coproporphyrinogen to protoporphyrinJournal of bacteriology4935319similar in allcells, this reaction should be demonstrable in all heme-containing bacteria. In addition, we also partially characterized the enzyme system in E. coli, which converts copropor Save
1970Brooks, JE;Disuse atrophy of muscle. Intracellular electromyographyArchives of neurology5409598The amount of depolarization necessary to induce an action potential was measured in allcells stimulated. The critical depolari¬ zation (CD) in normal fibers ranged from 6 mv to 36 mv Save
1970Jayaraman, S;Radiation Damage and Repair After Wholebody Or Partial-body Irradiations of Tenebrio Molitor LarvaeThesisBecause during the larval metamorphosis, not allcells divide simultaneously and because their growth is discon¬tinuous, it is conceivable that exposures that do not cause thedeath of Save
1970Mehall, AG;Capacity of anterior pituitary grafts to correct modified growth and development of selected long bones of hypophysectomized chick embryosThesis3 no morphological differentiation in limbbud; shapes of bones pre-determlned; allcells of bud equivalent and may formcartilage or muscle up to stage 22 Save
1970Jones, JM;Schwab, JH;Effects of streptococcal cell wall fragments on phagocytosis and tissue culture cellsInfection and immunity16557722Allcells containing one or more bacteria were counted. No correction was attempted for bacteriawhich may have only settled on top of phagocytic cells and were not taken up, since it Save
1969McClure, HM;Belden, KH;Pieper, WA;Jacobson, CB;Autosomal trisomy in a chimpanzee: resemblance to Down's syndromeScience (New York, N.Y.)4240970Cells containing fewer than 49 chromosomes probably resulted from excess spreading during hypotonic treatment, since allcells showed apparent trisomy of a small acrocentric. This Save
1969Whitman, MA;Discrimination Learning as a Function of MA, IQ, and InstitutionalizationThesisnumber of correct responses for the groupswhich were involved in computing these 5 tests are listed in Table 4(see Appendix 2 for average performance and average latency of allcells) Save
1969Jacoby, RO;Studies in canine immunobiology with special reference to mechanisms of lymphoid cell depletion in viral diseaseThesisIn virus-induced tumors, allcells transformed by a given v irus have a common TSTA regardless of the morphology of the tumor or its host of or ig in , but the TSTAs of tumors induced by Save
1969Gordon, MW;Neuronal plasticity and memoryThe American journal of orthopsychiatry4896079Some have been mentioned which are associated with the synaptic mem brane but allcells contain enzymes that direct incoming and outgoing metabolic traffic. Recently, an Save
1969Grady, CP;Gaudy, AF;Control Mechanisms Operative in a Natural Microbial Population Selected for Its Ability to Degrade L-Lysine. II. Effects of Fructose and Ribose in Batch SystemsApplied microbiology16349866Hence, not allcells of the population were capable of utilizing ribose. Since this had not been encountered with the carbon sources previously used, further selection was not carried out. Save
1969Baron, DN;Down with plasma. Intracellular chemical pathology studied by analysis of cells of solid tissues, erythrocytes, and leukocytesProceedings of the Royal Society of Medicine4900112For allcells, because of the technical problems involved, it had been usual to consider the cells with their accompanying extracellular fluid as a single tissue. This assumed, not neces Save
1968Bernheimer, AW;Cytolytic toxins of bacterial origin. The nature and properties of cytolytic proteins are discussed with emphasis on staphylococcal alpha-toxinScience (New York, N.Y.)4295145All cells that are sensitive to streptolysin 0 contain cholesterol as a constituent of their cell membranes, and allcells so far examined that lack membrane cho- Save
1968Bolognesi, DP;Langlois, AJ;Sverak, L;Bonar, RA;Beard, JW;In vitro chick embryo cell response to strain MC29 avian leukosis virusJournal of virology4301506has disclosed cultural changes of two principal kinds: (i) behavior of the virus-exposed cells paralleling that of the control cultures related presumably to environmental effects on allcells Save
1968Fielding, S;Auditory Adaptation: Evidence of DuplexityThesisThis 2 0 - ce ll experiment was rep lic a te d fiv e tim es; allcells in one replicat io n were completed b efo re the nex treplicat io n began. Save
1968Walters, RA;Petersen, DF;Radiosensitivity of Mammalian Cells: I. Timing and Dose-Dependence of Radiation-Induced Division DelayBiophysical JournalIt appears that not only do allcells recover the ability to traverse thelife cycle and divide after irradiation with relatively small doses but do so for significant periods of time without Save
1968Bloodworth, JM;Wers, KL;The ultrastructure of the normal dog adrenalJournal of anatomy5656137Adrenal capsule composed of elongated connective tissue cells (CT), basement membrane (BM) which surrounds most but not allcells (arrow), and collagen fibrils (C). x 6500. Fig. Save
1967Epel, D;Protein synthesis in sea urchin eggs: a "late" response to fertilizationProceedings of the National Academy of Sciences of the United States of America5232369Furthermore, autoradiographic data show that this protein synthesis occurs in allcells of the population, so that the observed incorporation cannot be attributed to the presence of Save
1967Carstensen, EL;Smearing, RW;Dielectric properties of osmium-fixed erythrocytesIEEE transactions on bio-medical engineering6078983Above 50 iIHz, the effective homiiogeneous conductivity for allcells described in Figs. and 3 is equal to the internal conductivity. The reactance of the imiembrane is negligible above Save
1967Panteleakis, PN;STUDIES OF NUCLEOLAR MORPHOLOGY AND CYTOCHEMISTRY AFTER ALTERATION OF RNA, AND PROTEIN SYNTHESISThesisS ince allcells tr ea te d in thism anner g iv es im ila r results , con tr o ls w ill not be repor te The firsteffectof actin omy cin D on allcells , w ith th eexcep tio nof HEp. No. 2 become d is Save
1967Hoff, BJ;The Cytology and Fertility of Bermudagrass, Cynodon Dactylon (L.) Pers., in ArizonaThesisHowever this was not the case in allcells and in cells from plant 41, with 3 accessory chromo¬somes, sometimes none, one, two or all three accessorychromosomes divided precociously Save
1967Tamura, A;Matsumoto, A;Higashi, N;Purification and chemical composition of reticulate bodies of the meningopneumonitis organismsJournal of bacteriology6025310When- almost allcells were infected, they were theni harvested, andthe cells were renioved by centrifugation at 300 X g for 10 nmimi. The organisimis in the superinatanit fluid were Save
1967Neill, C;Guest, WC;Effects of Hydroxyurea on Cultured Somatic Cells of the Chinese Hamster, Cricetulus GriseusThesisAllcells within the grid and the cells in some stage of cell division were scored. Ten randomly chosen fields were counted on each replicate. A mitotic index, calculated according to the Save
1966Gartland, WJ;Sueoka, N;Two interconvertible forms of tryptophanyl sRNA in E. coliProceedings of the National Academy of Sciences of the United States of America5327074Induction of cellular DNA synthesis by polyoma virus had so far been observed under conditions where not allcells were productively infected.In contrast with these findings, Sheinin Save
1966Vogt, M;Dulbecco, R;Smith, B;Induction of cellular DNA synthesis by polyoma virus. 3. Induction in productively infected cellsProceedings of the National Academy of Sciences of the United States of America4286763Induction of cellular DNA synthesis by polyoma virus had so far been observed under conditions where not allcells were productively infected.In contrast with these findings, Sheinin Save
1965Marcus, PI;Carver, DH;Hemadsorption-negative plaque test: new assay for rubella virus revealing a unique interferenceScience (New York, N.Y.)4283964In contrast, Echo-ll, polio-I, vaccinia, and influenza B viruses produce gross cytopathic changes in essentially allcells of the monolayer within 24 hours. A noncytopathic, hemadsorbing Save
1965Barker, CL;Maternal childrearing history and subsequent sensitivity to shame in college malesThesisLevel of illu m in atio n scores and level of sound scores co rre la te dpos itiv e ly in allcells , but only one of the eight £ 's was s ig n ific an t. O ther than this , th ere appeared to be no sys Save
1965MAC DONALD, C;AUTORADIOGRAPHY OF THYMIDINE-TRITIUM AND LYSINE-TRITIUM INCORPORATED INTO HUMAN CHROMOSOMESThesisThe process of duplication of cellular organellesimplied by the wcell theory" and the notion that allcells are derived from other cells, stands as a funda¬ mental concept of biology. The Save
1964ZIEVE, PD;GAMBLE, JL;JACKSON, DP;EFFECTS OF THROMBIN ON THE POTASSIUM AND ATP CONTENT OF PLATELETSThe Journal of clinical investigation14223918platelets produced by thrombin apparently was not due to a general effect on allcells, since thrombin did not induce a loss of potassium from red blood cells. The mechanism of action of Save
1964HINZE, HC;WALKER, DL;RESPONSE OF CULTURED RABBIT CELLS TO INFECTION WITH THE SHOPE FIBROMA VIRUS. I. PROLIFERATION AND MORPHOLOGICAL ALTERATION OF THE INFECTED CELLSJournal of bacteriology14219036Typical cytoplasmic inclu-sions appear at this time in virtually allcells of the infected culture. No other changes in cell morphology are apparent at this time. Eosin and hematoxylin stain. Save
1962Weiss, JE;THE EFFECT OF PROFESSIONAL TRAINING, AMOUNT OF INFORMATION, AND CORRECTNESS OF INFORMATION ON ACCURACY OF PREDICTION.Thesisd id not d is tr ib u te evenly over allcells , it was n ecessary Save
1960Kallenbach, E;DNA synthesis in the cells of the thymic cortex of the rat as shown in radioautographs after administration of Thymidine-H^ 3.ThesisSainte-Marie and Leblond (1958a) COQ~ted allcells seen in section that is also calottes of cells which were lying with the greater part of their volume outside the section. This procedure Save
1959Higgins, TW;New Polyacrylamide Gel Separators for the Le Clanche CellThesisP r io r to d is charge the open circuit vo ltage of allcells was measured to avoid te st in g an obviously de fec tiv ecell .The cel ls were prepared ford ischarge by so ld e rin g sh o rt lead Save
1958MANDELSTAM, J;Turnover of protein in growing and non-growing populations of Escherichia coliThe Biochemical journal13535591From the data available it is not possible to tell whether turnover occurs in allcells of a nongrowing population or whether all proteins are involved. A number of observations, however, Save
1957Somerville, RL;In vitro studies of purine nucleotide biosynthesis in rat intestinal mucosaThesisthe various forms of living matter pass on to their progeny their various specifically organized patterns of growth and metabolism and are prominent constituents of the nuclei of allcells (2 Save
1955ISING, U;Chromosome studies in Ehrlich mouse ascites cancer after heterologous transplantation through hamstersBritish journal of cancer133042184, which also suggests a narrowing of the scatter of the chromo-some numbers, the modal class containing as many as 39 per cent of allcells, as against only 25 per cent in the mouse Save
1955Angelotti, R;Studies on the physiological activities of terramycin-resistant and terramycin-susceptible cultures of cheese-ripening strains of Streptococcus lactisThesisUpon exposure to phage, allcells would presumably be sensitive and the number of resist¬ant bacteria obtained from a series of similar samples shouldshow only the fluctuation due to Save
1951Deschner, EE;The Relationship Between Ribonucleic Acid and the Gram Staining ReactionThesis, the morphology of the cells was not changed but some of the cells were completely Gram negative in character, yet no signs of any stages of digestion were present, that is, allcells Save
1951Williams, JLD;Enchondroma of the Nasal Cavities, and its Relationship to Other Cartilaginous Tumours in This RegionThesisnantof neop lasm s, us in g th eparasiticfoe tu ses , tera to ma ta and mixed tum ours as s te pp in gs to nes * Thus, al th ough th ereisanaturalfacility forlimitlessg ro w th in near ly allcells Save
1950Smith, GM;Regnery, DC;Inheritance of Sexuality in Chlamydomonas ReinhardiProceedings of the National Academy of Sciences of the United States of America16578332For all species investigated he finds exceptional cases in which allcells produced by a germinating zygote are homothallic (monoecious) instead of all being heterothallic. For zygotes Save
1950Jehle, H;Specificity of Interaction Between Identical MoleculesProceedings of the National Academy of Sciences of the United States of America16578331For all species investigated he finds exceptional cases in which allcells produced by a germinating zygote are homothallic (monoecious) instead of all being heterothallic. For zygotes Save
1941Loofbourow, JR;Dwyer, CM;Cronin, AG;Proliferation-promoting intercellular hormones: Evidence for their production by living cells as a response to injuryThe Biochemical journal16747427Both were then irradiated in quartz test tubes with full ultraviolet until allcells in the unboiled suspension were killed (methylene.blue test). The irradiation time in the experiment Save
1934Tryon, R;A correlation form with absolute checks.Journal of Experimental PsychologyIn each row of quadrant 1, Multiply the frequency in each cell tines the nuwber in the upper corner of the cell, ant) SUB all these for allcells in that row, placing the total In the proper Save
1929McDonald, E;CELLULAR DIVISION IN RELATION TO CANCERScience (New York, N.Y.)17733743The cell reacts as a unit and there are certain phenomena which are common to allcells. There are regularities in the complexity of cell phenomena which make it possible to study cell Save
1926Orahovats, D;The spleen and the resistance of red cellsThe Journal of physiology16993804First a group which is most resistant and which forms about 10 pc of allcells, then a group to which the greatest part of the red cells belong, about 80 pc, with a middle resistance, and a Save
1920Mott, FW;Studies in the Pathology of Dementia PraecoxProceedings of the Royal Society of Medicine19981338This applies to allcells including those of the olivary body, although the pigment in this kind of cell is finer. The large cells show the lipoid granules better. Unstained Specimen of Medulla Save
1911Paine, A;Nicholson, GW;SURGICAL INTERFERENCE IN CANCER: (AN EXPERIMENTAL STUDY.)British medical journal20765743As allcells have an age limit, at the end of which they die and towards the close of whichthey become old and their activities diminish, itfollows that the isolated nodules, which consist Save
1905MARCY, H;THE CURE OF HERNIA.Journal of the American Medical Associationfor 30 minutes and not allcells being killed during one hour at this temperature. This may explain the fact that the virus is not always killed by cooking the meat. The organism probably Save
1874Mahomed, FA;The Relation between Arterial Tension and AlbuminuriaBritish medical journal20747530These are cast off in great numbers, and, blocking the tubules, form the large white kidney; here they undergo the change common to allcells after their death-namely, molecular Save
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