Mesenchymal Cell Expansion

Bone Marrow Derived           Mesenchymal Cells Cell Number** Fold Expansion
*Passage 1 (day 0) 1.25 x 105 start
  Passage 2 (day 5) 4.75 x 105 3.5
  Passage 3 (day 10) 15.3 x 105 12.2
  Passage 4 (day 15)    71 x 105 57
  Passage 5 (day 20) 336 x 105 269
  Passage 6 (day 25) 940 x 105 752
  Passage 7 (day 30) 3855 x 105 3084

 

*Passage 1 represents the first harvest of mesenchymal cells (day 10) from starting cell culture using bone marrow mononuclear cells.

 Passaged cells plated at 5000/cm2 and passaged at 70% confluency.

**Cell numbers are a representation of typical expansion using ReachBio's MSC Complete Medium.