ColonyGEL™Methylcellulose-based Media for CFC Assays
CFC Assay Media
ColonyGEL™ methylcellulose-based media is optimized for the differentiation and enumeration of myeloid, erythroid and mixed-lineage hematopoietic progenitors from bone marrow, cord blood, mobilized peripheral blood, peripheral blood, mouse fetal liver and mouse spleen cells. Enriched cell populations such as CD34+ and CD133+ cells can also be used in ColonyGEL™ media.
Using only the highest quality , pre-screened components, ColonyGEL™ is guaranteed to meet or exceed the performance of the current gold standard media (Table l). ColonyGEL™ combines uncompromising quality and performance with GREAT value.
ColonyGEL™️ PerformanceMeets or Exceeds the Gold Standard
Table 1. CFC assays were initiated in a methylcellulose-based medium containing rh-lL-3, rh-GM-C SF, rh-SCF and Epo and using cells derived from normal human bone marrow (2 x 104/culture ) , cord blood (l x 104/ culture) and mobilized peripheral blood (5 x l 04 / culture). Erythroid (BFU-E) , myeloid (CFU-GM) and multi-potential (CFU-GEMM) progenitors were enumerated following 14 days in culture.
*ND - None Detected. Colonies derived from CFU-E are not typically found in cord blood and mobilized peripheral blood. CFU-GEMM -derived colonies are extremely rare and there fore , may not be detected.