Colonies ID

Colony Identification Guide

DEFINITIONS

CFU-GM: Colony-Forming Unit - Granulocyte Macrophage is a myeloid progenitor that produces a colony containing >40 granulocyte and macrophage cells.

CFU-G: Colony-Forming Unit - Granulocyte is a myeloid progenitor that produces a colony with >40 granulocytes.

CFU-M: Colony-Forming Unit – Macrophage is a myeloid progenitor that produces a colony with >40 macrophages. BFU-E: Burst-Forming Unit - Erythroid is an erythroid progenitor that produces a colony with >200 erythroblasts in >2 clusters.

BFU-E: progenitors are more primitive and have high proliferative potential, generally forming large colonies that are visibly red in color.

CFU-E: Colony-Forming Unit - Erythroid is an erythroid progenitor that produces a colony containing 1 or 2 clusters of 8-200 erythroblasts.

CFU-E: progenitors are less primitive, have lower proliferative potential and therefore, colonies are generally small when compared to colonies derived from BFU-E.

CFU-GEMM: Colony-Forming Unit - Granulocyte Erythroid Macrophage Megakaryocyte is a mixed lineage progenitor that produces a colony containing >200 erythroblasts as well as 20 or more granulocyte, macrophage and megakaryocyte cells.

CFU-MM: Colony-Forming Unit - Multiple Myeloma Leukemic Progenitors producing a range of colony numbers. Confirmation through surface phenotype (plucked colonies) CD138+.

ColonyGEL™️ PRODUCT LIST

ColonyGEL1

ColonyGEL2