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Assays > Quantification of Stem Cells
Quantification of Stem Cells in NOD/SCID Model
The NOD/SCID mouse system is a powerful tool to assess true stem cell number and functional behavior. Although in vitro assays such as
Colony Forming Cell (CFC) assays are extremely useful in enumerating and analyzing hematopoietic and non-hematopoietic progenitors, the acid test for true stem cells is their ability to reconstitute the entire blood forming system of an ablated host. The NOD/SCID mouse is an immunodeficient recipient, which allows the introduction of human cells and the determination of stem cell functionality through engraftment, proliferation and differentiation into at least two distinct lineages (typically myeloid and lymphoid). This in vivo reconstitution assay is typically known as the
Competitive Repopulating Unit (CRU) or SCID
Repopulating Cell (SRC)
assay.
ReachBio can perform CRU/SRC assays with NOD/SCID mice to:
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Quantify true stem cell numbers in test and control samples of human cells
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Determine the effect of test compounds on engraftment potential of human stem cell samples
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Quantify functional stem cell numbers in
expanded cell populations
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ReachBio’s comprehensive service using the in vivo NOD/SCID model (CRU/SRC assays) includes:
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Please
contact us
to discuss your needs.
* ReachBio Study Directors are highly knowledgeable and have many years of experience in the scientific and practical aspects of CRU/SRC assays.
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