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Home > Media for CFC
Assays
Media for CFC Assays
About Colony Forming Cell Assays
Hematopoietic progenitors with lineage-restricted (less primitive) or multi-lineage (more primitive) potential form morphologically distinct colonies when grown in a suitable semi-solid medium containing appropriate growth factors and other supplements. To form the specific colony type that characterizes its potential, a progenitor cell must both proliferate and differentiate within the supportive matrix. Colony-forming cell (CFC) assays therefore have great utility in the identification and enumeration of the different types of functional progenitors in hematopoietic tissues (e.g., bone marrow, cord blood, fetal liver) and manipulated cell populations (e.g., purified, expanded, frozen/thawed, drug treated, etc.).
For a variety of reasons, methylcellulose has become the matrix of choice for semi-solid media for CFC assays. Pre-screening and qualification of the various components of methylcellulose-based media allow for media products that are highly reproducible in their ability to support vigorous and robust growth of the various types of progenitors. ColonyGEL™ meets the highest standards of methylcellulose-based media for hematopoietic colony-forming cell assays.
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About ColonyGEL™
We know colony assays!
ReachBio has been performing hematopoietic progenitor/colony-forming cell (CFC) assays and other primary cell-based assays under contract for premier biotechnology and pharmaceutical companies since 2007. Our scientists have decades of prior experience performing CFC assays in clinical, industrial and academic settings. Our Chief Scientific Officer,
Dr. Emer Clarke, has trained countless individuals around the world on hematopoietic and mesenchymal CFC assays. She also designed and managed the first global proficiency testing program for standardizing hematopoietic CFC assays. Since our inception, we have used our ColonyGEL™ media for our contract services for such high value industrial applications as drug screening, hemotoxicity testing, cancer stem cell assays, cytokine mimetic identification, evaluation of expanded cell populations, monitoring mobilization kinetics, etc. We are now making our high quality methylcellulose-based ColonyGEL™ media widely available to the broader research community. We guarantee
that its performance will meet or exceed any media of its kind on the market today!
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Features and Benefits
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Pre-screened and carefully selected components
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Reproducible and reliable - used in ReachBio's own contract service labs for high value drug development applications
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Great optical clarity
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Manufactured in the USA in accordance with good manufacturing practices (GMP)
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Meets or beats the performance of the current gold standard CFC media
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Various optimized formulations available
- with or without Epo
- standard or enhanced cytokine
content
- species-specific formulations -
each performance tested with
primary progenitor cells of
the relevant species
- base medium also available –
allows addition of your own growth
factors for unique
application -
Suitable for identifying and enumerating:
- CFU-E
- BFU-E
- CFU-G
- CFU-M
- CFU-GM
- CFU-GEMM
- Pre-B (mouse only)
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